title: Conserved lipoprotein H.8 of pathogenic Neisseria consists entirely of pentapeptide repeats.
abstract: The pathogenic Neisseria, N. gonorrhoeae and N. meningitidis, possess an outer membrane protein (OMP), designated H.8, with a conserved monoclonal antibody (MAb)-binding epitope. We determined the DNA sequence of a gonococcal H.8 gene, and confirmed the relationship between the cloned gene and the H.8 OMP by constructing a gonococcal mutant lacking H.8. The predicted H.8 OMP is a lipoprotein 71 amino acids in length, composed of 13 repeats of a consensus sequence AAEAP with perfect 5-residue periodicity. The AAEAP units form a repeating epitope that comprises the entire predicted sequence of the protein.
authors.affiliation: Department of Microbiology and Immunology, School of Medicine, University of North Carolina, Chapel Hill 27599.
authors.forename: J P
authors.initials: JP
authors.lastname: Woods
authors.forename: S M
authors.initials: SM
authors.lastname: Spinola
authors.forename: S M
authors.initials: SM
authors.lastname: Strobel
authors.forename: J G
authors.initials: JG
authors.lastname: Cannon
mesh_terms: D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D005796:Genes; D005798:Genes, Bacterial; D015151:Immunoblotting; D008074:Lipoproteins / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D009344:Neisseria gonorrhoeae / Q000235:genetics*; D009345:Neisseria meningitidis / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; C055700:H.8 protein (Neisseria); D008074:Lipoproteins