Stimulation of microtubule assembly by recombinant human interferon-alpha and interferon-gamma.
Interferon-alpha (IFN-alpha) and interferon-gamma (IFN-gamma) have been demonstrated to stimulate microtubule assembly measured in the in vitro assembly system. The process is substoichiometric occurring when the interferon concentrations are below that of tubulin. IFN-gamma is a more potent effector than IFN-alpha. The critical tubulin concentration describing microtubule assembly decreases from 1.5 mg/ml measured in the absence of added effector to 1.05 mg/ml and 1.3 mg/ml when measured in the presence of 2.16.10(-6) M IFN-gamma and 3.06.10(-6) M IFN-alpha, respectively.
Ward
L D
LD
Graduate Department of Biochemistry, Brandeis University, Waltham, MA.
Arakawa
T
T
D000818:Animals; D002417:Cattle; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology*; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism*; D011994:Recombinant Proteins; D013050:Spectrometry, Fluorescence; D014404:Tubulin / Q000378:metabolism*
D007370:Interferon Type I; D011994:Recombinant Proteins; D014404:Tubulin; D007371:Interferon-gamma