Adipocyte plasma-membrane Gi and Gs in insulinopenic diabetic patients.
Changes in the amounts and functions of G-proteins have been reported in diabetic rats. We determined the Gi/Gs ratio of adipocyte plasma membranes in six insulinopenic diabetic patients and matched controls by immunoblotting with antisera against synthetic peptides corresponding to regions of G-proteins. No consistent changes in the Gi/Gs ratio were observed. It is still possible that the functional status of the G-proteins may be altered in diabetes.
Ohisalo
J J
JJ
Department of Medical Chemistry, University of Helsinki, Finland.
Vikman
H L
HL
Ranta
S
S
Houslay
M D
MD
Milligan
G
G
D000273:Adipose Tissue / Q000378:metabolism*; D015153:Blotting, Western; D002462:Cell Membrane / Q000378:metabolism; D003920:Diabetes Mellitus / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006801:Humans; D007328:Insulin / Q000502:physiology*
D007328:Insulin; D019204:GTP-Binding Proteins