A gamma interferon-unresponsive variant of cell line 70Z/3, IFN-4, can be partially rescued by phorbol myristate acetate.
Lipopolysaccharide (LPS) and gamma interferon (IFN-gamma) induce kappa transcription in 70Z/3 cells by different mechanisms; LPS treatment induces both NF-kappa B and OTF-2 transcription factors, but IFN-gamma treatment does not. We have dissected these two activation pathways by selecting and analyzing an LPS+ IFN- variant of 70Z/3.
Emery
D W
DW
Department of Genetics, University of Washington, Seattle 98195.
Rooney
J W
JW
Sibley
C H
CH
D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D004268:DNA-Binding Proteins; D004351:Drug Resistance; D005803:Genes, Immunoglobulin; D007145:Immunoglobulin kappa-Chains / Q000235:genetics; D007371:Interferon-gamma / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D016328:NF-kappa B; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014157:Transcription Factors / Q000235:genetics*
D004268:DNA-Binding Proteins; D007145:Immunoglobulin kappa-Chains; D008070:Lipopolysaccharides; D016328:NF-kappa B; D014157:Transcription Factors; D007371:Interferon-gamma; D013755:Tetradecanoylphorbol Acetate