Identification of S-100 proteins and S-100-binding proteins in a detergent-resistant EDTA/KCl-extractable fraction from bovine brain membranes.
The Triton X-100-resistant residue of brain membranes contains appreciable amounts of S-100 proteins. This fraction of S-100 can be solubilized by high concentrations of EDTA plus or minus high concentrations of KCl. Whereas KCl (0.6 M) extracts the detergent-resistant S-100, NaCl (1 M) does not. Endogenous Ca2+ is required and is sufficient for S-100 to remain associated with the detergent-resistant residue. However, 0.6 M KCl extracts a further fraction of Triton X-100-resistant S-100. In contrast, the Triton X-100-extractable fraction of S-100 resists the action of EDTA. These data suggest that Ca2+ regulates the extent of association of S-100 with Triton X-100-resistant components in brain membranes, whereas the association of S-100 with the lipid bilayer of brain membranes and/or with some intrinsic membrane proteins is less Ca2+-regulated. Several S-100-binding proteins are identified in the detergent-resistant residue of brain membranes by an overlay procedure.
Donato
R
R
Section of-Anatomy, Department of Experimental Medicine and Biological Sciences, Perugia, Italy.
Giambanco
I
I
Aisa
M C
MC
Ceccarelli
P
P
D000818:Animals; D001923:Brain Chemistry; D002122:Calcium Chloride / Q000494:pharmacology; D002352:Carrier Proteins / Q000032:analysis*; D002417:Cattle; D002462:Cell Membrane / Q000032:analysis / Q000378:metabolism; D002845:Chromatography; D003599:Cytoskeleton / Q000378:metabolism; D004492:Edetic Acid / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D015151:Immunoblotting; D008970:Molecular Weight; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology*; D011189:Potassium Chloride / Q000494:pharmacology*; D009418:S100 Proteins / Q000032:analysis* / Q000378:metabolism
D002352:Carrier Proteins; D009418:S100 Proteins; D011092:Polyethylene Glycols; D011189:Potassium Chloride; D017830:Octoxynol; D004492:Edetic Acid; D002122:Calcium Chloride