In vitro responsiveness of human testicular DNA synthesis to gonadotropins.
To evaluate the responsiveness of spermatogenesis in the normal human testis to human menopausal gonadotropin (hMG) and human chorionic gonadotropin (hCG), the DNA synthesis of spermatogonia was investigated using 3H-thymidine uptake by cultured tissue fragments of human testis with or without treatment with hCG and/or hMG. Two groups treated with different concentrations of hCG showed no difference in 3H-thymidine incorporation into testicular tissue compared with untreated control. In two other groups treatment with combined hMG/hCG in different concentrations induced significant changes in 3H-thymidine incorporation compared with control (p less than 0.01, p less than 0.01). Combined hMG/hCG treatment was effective in inducing spermatogenesis by normal human testicular tissue.
Okuyama
A
A
Department of Urology and Pathology, Osaka University Medical School, Japan.
Nonomura
N
N
Koh
E
E
Kondoh
N
N
Nakamura
M
M
Namiki
M
M
Kiyohara
H
H
Nishimune
Y
Y
Matsumoto
K
K
Sonoda
T
T
D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D046508:Culture Techniques; D004247:DNA / Q000096:biosynthesis*; D006801:Humans; D008297:Male; D008596:Menotropins / Q000494:pharmacology*; D013091:Spermatogenesis / Q000187:drug effects; D013737:Testis / Q000187:drug effects / Q000378:metabolism*
D006063:Chorionic Gonadotropin; D008596:Menotropins; D004247:DNA