The structure of the O-polysaccharide from Pseudomonas aeruginosa IID 1009 (ATCC 27585).
Structural studies were carried out on the O-polysaccharide fraction obtained by mild acid treatment of the lipopolysaccharide from Pseudomonas aeruginosa IID 1009 (ATCC 27585). The O-polysaccharide was composed of L-rhamnose, N-acetyl-D-quinovosamine, and N-acetyl-L-galactosaminuronic acid in a molar ratio of 1:1:1. The results from analysis of fragments obtained by hydrogen fluoride hydrolysis of O-polysaccharide, together with data on methylation analysis and nuclear magnetic resonance spectroscopic analysis, led to the most likely structure of the repeating units of the polymer chain ----4)L-GalNAcA(alpha 1----3)D-QuiNAc(alpha 1----3)L-Rha(alpha 1----, in which about 70% of the rhamnose residues were O-acetylated at C-2. This structure coincides with that of the repeating unit of Lanyi 02 a,b polysaccharides.
Kaya
S
S
Department of Chemistry, Faculty of Science, Hokkaido University.
Araki
Y
Y
Ito
E
E
D002240:Carbohydrate Sequence; D008070:Lipopolysaccharides; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial; D011550:Pseudomonas aeruginosa / Q000378:metabolism*
D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial