[Enumeration and identification of Clostridium from stools treated with the thioglycollate-lysozyme method].
We have used spore isolation by the sodium thioglycolate-lysozyme technique on collected stools. Of the 51 stools studied, we found 41% of Clostridium perfringens with an average ratio of 10(4) germs/gr. 15 strains were typical double hemolysis and trehalose positive-5 presented only one hemolysis and were trehalose negative. We only found a single strain of Clostridium difficile with a rate of 10(4) germs/gr in the stools of a 10 months infant.
Merad
S
S
Boughenou
D
D
D000293:Adolescent; D000328:Adult; D001431:Bacteriological Techniques; D002648:Child; D002675:Child, Preschool; D003013:Clostridium / Q000187:drug effects / Q000302:isolation & purification*; D003470:Culture Media; D004352:Drug Resistance, Microbial; D005243:Feces / Q000382:microbiology*; D006801:Humans; D007223:Infant; D009113:Muramidase; D013171:Spores, Bacterial; D013864:Thioglycolates
D003470:Culture Media; D013864:Thioglycolates; C017487:2-mercaptoacetate; D009113:Muramidase
Numération et identification des Clostridium à partir de selles traitées par la methode au thioglycolate-lysozyme.