Unambiguous typing for HLA-DQ TA10 and 2B3 specificities using specific oligonucleotide probes.
Oligonucleotide probes specific for the serologically defined TA10 and 2B3 specificities were selected based on a comparison of the available HLA-DQ beta sequences. Panel and family segregation studies confirm a complete correlation between the reactivities of the selected probes and the TA10/IIB3 antibodies. The Glu residue at position 45 of the HLA-DQ beta chain is specific for the TA10 determinants, and a DQ beta Gly-Val-Tyr sequence is found at position 45-47 for all 2B3-positive DQ beta chains.
Kenter
M J
MJ
Department of Immunohaematology, University Hospital, Leiden, The Netherlands.
Anholts
J D
JD
Schreuder
G M
GM
van Eggermond
M C
MC
Ghyselen
G M
GM
van Rood
J J
JJ
Giphart
M J
MJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D015342:DNA Probes; D015343:DNA Probes, HLA; D005260:Female; D005802:Genes, MHC Class II; D006683:HLA-DQ Antigens / Q000235:genetics*; D059866:HLA-DQ beta-Chains; D006650:Histocompatibility Testing; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data
D015342:DNA Probes; D015343:DNA Probes, HLA; D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C057615:HLA-DQbeta antigen