title: Recombinant peptides derived from the env-gene of HIV-2 in the serodiagnosis of HIV-2 infections.
abstract: We produced recombinant envelope-derived peptides of HIV-2 for use in a diagnostic enzyme-linked immunosorbent assay (ELISA) or Western blot by expressing several restriction enzyme fragments from the env gene of HIV-2 in the bacterial fusion vector pEX-3. On Western blots, 17 out of 18 anti-HIV-2-positive sera available to us reacted strongly with those recombinant peptides which were derived from, or extended into, the transmembrane protein of HIV-2. In contrast, recombinant peptides derived from the external envelope glycoprotein were only weakly recognized by two sera. We observed a cross-reactivity of some human sera containing antibodies to HIV-1 with the HIV-2 peptides derived from the transmembrane protein of HIV-2. In spite of this cross-reactivity, a serological distinction between anti-sera to HIV-1 and HIV-2 can be attempted by simultaneous testing in ELISA on recombinant peptides derived from the transmembrane protein of HIV-1 and HIV-2.
authors.affiliation: Boltzmann Institute for AIDS Research, Innsbruck, Austria.
authors.forename: T F
authors.initials: TF
authors.lastname: Schulz
authors.forename: W
authors.initials: W
authors.lastname: Oberhuber
authors.forename: J M
authors.initials: JM
authors.lastname: Hofbauer
authors.forename: P
authors.initials: P
authors.lastname: Hengster
authors.forename: C
authors.initials: C
authors.lastname: Larcher
authors.forename: L C
authors.initials: LC
authors.lastname: Gürtler
authors.forename: R
authors.initials: R
authors.lastname: Tedder
authors.forename: H
authors.initials: H
authors.lastname: Wachter
authors.forename: M P
authors.initials: MP
authors.lastname: Dierich
mesh_terms: D015492:AIDS Serodiagnosis; D015153:Blotting, Western; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D015483:HIV Antibodies / Q000032:analysis; D015498:HIV-2 / Q000235:genetics / Q000276:immunology*; D010957:Plasmids; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D014760:Viral Fusion Proteins / Q000032:analysis
chemical_list: D015483:HIV Antibodies; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins