Pyruvate kinase during in vitro differentiation of GM-CFC haemopoietic precursor in mice: modulation by L-alanine and L-phenylalanine.
Pyruvate kinase modulation by L-alanine and L-phenylalanine was studied in cells obtained from in vitro culture of GM-CFC at different stages of development. L-Alanine and L-phenylalanine exhibit different inhibition behaviour at both differentiation stages and at various PEP concentration ranges. Therefore, a change in PK expression during granulocyte-macrophage development is suggested.
Cuenllas
E
E
Departamento de Bioquímica, Facultad de Veterinaria, Universidad Complutense de Madrid, Spain.
Gaitan
S
S
Bueren
J A
JA
Tejero
C
C
D000409:Alanine / Q000494:pharmacology*; D000818:Animals; D002454:Cell Differentiation; D006098:Granulocytes / Q000201:enzymology*; D066298:In Vitro Techniques; D007700:Kinetics; D008264:Macrophages / Q000201:enzymology*; D051379:Mice; D010649:Phenylalanine / Q000494:pharmacology*; D011770:Pyruvate Kinase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013379:Substrate Specificity
D010649:Phenylalanine; D011770:Pyruvate Kinase; D000409:Alanine