Glucocorticoids modulate mRNA levels for G-protein beta-subunits.
Adrenalectomy decreases, whereas glucocorticoid treatment increases, the steady-state levels of G-protein beta-subunits (G beta) in rat fat-cells. A DNA-excess solution-hybridization assay was established to define the steady-state mRNA levels for G beta [5.8 +/- 0.4 amol/micrograms of RNA (n = 5) in control fat-cells]. G beta mRNA levels decrease by 20% after adrenalectomy; dexamethasone treatment reverses the decline. Dexamethasone treatment itself increases G beta mRNA levels by 50%.
Ros
M
M
Department of Pharmacological Sciences, School of Medicine, State University of New York, Stony Brook 11794-8651.
Watkins
D C
DC
Rapiejko
P J
PJ
Malbon
C C
CC
D000273:Adipose Tissue / Q000032:analysis*; D000818:Animals; D003907:Dexamethasone / Q000494:pharmacology*; D019204:GTP-Binding Proteins; D012333:RNA, Messenger / Q000032:analysis / Q000187:drug effects*; D051381:Rats
D012333:RNA, Messenger; D003907:Dexamethasone; D019204:GTP-Binding Proteins