Potent contractile effect of endothelin in isolated guinea-pig airways.
Endothelin produced a concentration-dependent (1 nM-0.3 microM) contraction of isolated guinea-pig airways (trachea and main bronchi). The response was unaffected by tetrodotoxin (1 microM) and slightly depressed by indomethacin (5 microM) but promptly abolished by isoprenaline (1 microM) or EDTA (3 mM). In the bronchi, the response to endothelin was enhanced by removal (rubbing) of the epithelium. The response of the trachea or bronchi to endothelin (0.3 microM) was unaffected by NiCl2 (0.1 mM) or omega conotoxin (0.1 microM) but was partially inhibited by nifedipine (1 microM).
Maggi
C A
CA
Pharmacology Department, A Menarini Pharmaceuticals, Florence, Italy.
Patacchini
R
R
Giuliani
S
S
Meli
A
A
D000818:Animals; D001980:Bronchi / Q000187:drug effects; D004492:Edetic Acid / Q000494:pharmacology; D016232:Endothelins; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects*; D009543:Nifedipine / Q000494:pharmacology; D010455:Peptides / Q000494:pharmacology*; D013779:Tetrodotoxin / Q000494:pharmacology; D014132:Trachea / Q000187:drug effects
D016232:Endothelins; D010455:Peptides; D013779:Tetrodotoxin; D004492:Edetic Acid; D009543:Nifedipine; D007545:Isoproterenol; D007213:Indomethacin