title: Intravascular thrombosis: definitive detection by quantitative tissue characterization in vitro.
abstract: To improve the sensitivity and reliability of the ultrasonic detection of intravascular thrombosis, we employed quantitative ultrasonic techniques for the analysis of thrombi induced in vitro. Citrated human blood or platelet-rich plasma was added to sections of canine thoracic aorta mounted vertically in a saline bath. CaCl2 and thrombin were then added to induce thrombosis. Ultrasonic integrated backscatter measured sequentially with a 10-MHz focused transducer exhibited more than a 10 dB increase in integrated backscatter when either whole blood or platelet-rich plasma was clotted (-61.7 dB to -47.4 dB whole blood; less than -70 dB to -52.8 dB platelet-rich plasma). Thus, large significant changes in integrated backscatter measured from structures within the arterial lumen were readily detectable and indicative of thrombosis in situ.
authors.affiliation: Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri 63110.
authors.forename: B
authors.initials: B
authors.lastname: Barzilai
authors.forename: H E
authors.initials: HE
authors.lastname: Eisen
mesh_terms: D000328:Adult; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008854:Microscopy, Electron; D013927:Thrombosis / Q000175:diagnosis* / Q000473:pathology; D014463:Ultrasonography