title: DNA ploidy of human breast cancer.
abstract: Ploidy was determined on 663 resectable primary tumors from untreated patients. Nuclei obtained by mechanical disaggregation of frozen tissue were stained with propidium iodide and analysed in a FACS IV. Aneuploidy was detected in 73% of cases. It was not significantly related to nodal involvement or tumor size, although the highest frequencies were observed in large tumors (88%) or with more than 10 positive nodes (77%). Aneuploidy was more frequently observed in ductal infiltrating (81%) than in lobular histology and in tumors lacking both progesterone and estrogen receptors (85%). Analysis of ploidy in primary and synchronous lymph node metastases from the same patient showed a high agreement rate (90%) of DNA patterns simply defined as diploid or aneuploid. However, differences in DNA stemlines and DNA indices between the two synchronous lesions from the same patient were a rather frequent event.
authors.affiliation: Istituto Nazionale per lo Studio e la Cura dei Tumori, Milano, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Del Bino
authors.forename: R
authors.initials: R
authors.lastname: Silvestrini
authors.forename: M R
authors.initials: MR
authors.lastname: Zucconi
authors.forename: G
authors.initials: G
authors.lastname: Marchese
authors.forename: B
authors.initials: B
authors.lastname: Valentinis
authors.forename: G
authors.initials: G
authors.lastname: Di Fronzo
authors.forename: S
authors.initials: S
authors.lastname: Andreola
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001943:Breast Neoplasms / Q000737:chemistry / Q000235:genetics* / Q000473:pathology; D002458:Cell Fractionation / Q000379:methods; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005615:Freezing; D006801:Humans; D008207:Lymphatic Metastasis; D008875:Middle Aged; D011003:Ploidies; D011960:Receptors, Estrogen / Q000032:analysis; D011980:Receptors, Progesterone / Q000032:analysis
chemical_list: D004273:DNA, Neoplasm; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone