title: Expression of rubella virus cDNA encoding the E1 structural protein.
abstract: cDNAs synthesized from the 40S RNA genome of rubella virus were cloned into the lambda gt10 bacteriophage. A clone (pRV # 1475) which contains a truncated version of the E1 envelope glycoprotein (amino acid residues 17-481) and the 3' non-coding region was constructed from two overlapping cDNAs. pRV # 1475 was inserted into a eukaryotic expression vector under the control of the cytomegalovirus immediate early promoter. After transfection of 293 cells, a protein with intact antigenic domains could be detected.
authors.affiliation: Institut Gustave Roussy, Villejuif, France.
authors.forename: A
authors.initials: A
authors.lastname: de Mazancourt
authors.forename: M
authors.initials: M
authors.lastname: Perricaudet
mesh_terms: D002460:Cell Line; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000096:biosynthesis* / Q000235:genetics; D005455:Fluorescent Antibody Technique; D010957:Plasmids; D012367:RNA, Viral / Q000302:isolation & purification; D012412:Rubella virus / Q000235:genetics*; D014162:Transfection; D014759:Viral Envelope Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral; D014759:Viral Envelope Proteins