An improved high performance liquid chromatographic method for determination of cimetidine and its major metabolite in plasma.
An improved HPLC method for the measurement of cimetidine and its major metabolite-cimetidine sulfoxide-is described. The mobile phase was a mixture of methanol-ammonium phosphate (20:80), and methanol was used to elute the drug avoiding the use of acetonitrile, a very hazardous solvent. A device has been developed to wash simultaneously ten or more cartridges and to reduce the time of analysis. With this procedure a plasma sample could be analyzed in less than 30 min. The limit of detection was 0.125 micrograms/ml for cimetidine and 1.00 micrograms/ml for cimetidine sulfoxide. The absolute recovery for cimetidine was 83% and 40% for its major metabolite; is greater than that reported by previous studies. The proposed method is rapid, accurate and precise, and it should be useful for clinical, bioavailability and pharmacokinetic studies of cimetidine.
León-Urrea
F
F
Laboratorio de Farmacología y Desarrollo de Metodología Analítica, Jefatura de Control de Calidad, Instituto Mexicano del Seguro Social.
Girard-Cuesy
M E
ME
Cervantes-Campos
C
C
Delgadillo-Valencia
M P
MP
Montoya-Cabrera
M A
MA
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D002927:Cimetidine / Q000031:analogs & derivatives / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D000432:Methanol; D012997:Solvents
D012997:Solvents; D002927:Cimetidine; C034042:cimetidine sulfoxide; D000432:Methanol