A new mutant strain of Gunn-LA Wistar rats with genetic deficiencies in bilirubin and androsterone uridine diphosphate-glucuronosyltransferases.
Hooded Gunn rats which have low-activity of 4-nitrophenol uridine diphosphate-glucuronosyltransferase (GT) and defect in bilirubin GT were crossed with albino LA Wistar rats with a defect in androsterone GT. From F2 and F3 progeny were selected Gunn-LA Wistar rats which have defective bilirubin and androsterone GTs and low-active 4-nitrophenol GT. These rats had either hooded or albino coat color. In order to establish a uniform genetic background, albino male Gunn-LA Wistar rats were crossed with heterozygous albino female Gunn-LA Wistar rats in terms of bilirubin GT. The offsprings were all albino and were classified to Gunn-LA Wistar rats and heterozygous Gunn-LA Wistar rats by assaying their hepatic GT activities or by the appearance of jaundice. The linkage relationships between GT and coat color genes are discussed.
Nagai
F
F
Kyoritsu College of Pharmacy, Tokyo, Japan.
Homma
H
H
Matsui
M
M
D000818:Animals; D003433:Crosses, Genetic; D005260:Female; D014453:Glucuronosyltransferase / Q000172:deficiency / Q000235:genetics*; D006602:Hexosyltransferases / Q000235:genetics*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D051381:Rats; D011911:Rats, Gunn / Q000235:genetics; D011919:Rats, Inbred Strains / Q000235:genetics
C019787:bilirubin glucuronoside glucuronosyltransferase; D006602:Hexosyltransferases; D014453:Glucuronosyltransferase