title: [Studies on endotoxin of Leptospira. V. The effect of EDTA-Na on the release of the LPS of Leptospira interrogans serovar lai].
abstract: The yield of leptospiral LPS was able to increase 1-2 times when the cells were treated with EDTA-Na before extracting with hot phenal-water method. The colours and solubility of L-LPS were improved. When the leptospiral cells were stored for a long period of time, and treated by EDTA-Na, the phase distribution of the LPS changed, i.e. they returned from water phase into phenol phase. Such change was related to the quantity of both the saccharide and lipid.
authors.forename: S H
authors.initials: SH
authors.lastname: Wu
authors.forename: S X
authors.initials: SX
authors.lastname: Jiang
authors.forename: H Q
authors.initials: HQ
authors.lastname: Wang
authors.forename: D K
authors.initials: DK
authors.lastname: Nie
authors.forename: G F
authors.initials: GF
authors.lastname: Zhu
mesh_terms: D004492:Edetic Acid / Q000494:pharmacology*; D007921:Leptospira interrogans / Q000187:drug effects / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000378:metabolism*
chemical_list: D008070:Lipopolysaccharides; D004492:Edetic Acid