title: Opening of the blood-brain barrier by intravenous contrast media in euvolemic and dehydrated rabbits.
abstract: It has been suggested that intravenous injections of hypertonic contrast media when used in computed tomography and digital subtraction angiography might raise plasma osmolality sufficiently to open the blood-brain barrier (BBB). The current investigation establishes the threshold of plasma osmolality that causes the the opening of the BBB in euvolemic and dehydrated rabbits. Euvolemic rabbits were allowed food and water ad libitum. Dehydrated rabbits received 4.0 mg/kg of furosemide intramuscularly and were deprived of water for 72 hours. Meglumine/sodium diatrizoate 76 per cent (n = 28) or mannitol 20 per cent (n = 12) was administrated intravenously, at a rate of 25 mmol/kg body weight/hour for 2, 3 or 4 hours. Plasma osmolality, blood iodine concentration, blood pressure, heart rate and hematocrit were assessed at regular intervals. Evans blue and 99Tcm-DTPA were used simultaneously as tracers for BBB opening. Rating of BBB opening with 99Tcm-DTPA correlated well with Evans blue staining (r = 0.863, p less than 0.001; n = 42). BBB opening was related to plasma osmolality and was similar for both contrast media and mannitol. Widespread BBB opening occurred above 400 mmol/kg while focal BBB opening occurred above 370 mmol/kg. Dehydration per se increased plasma osmolality but did not reduce the threshold for BBB opening.
authors.affiliation: Department of Radiology, University of Rochester, School of Medicine and Dentistry, New York 14642.
authors.forename: K
authors.initials: K
authors.lastname: Hayakawa
authors.forename: T M
authors.initials: TM
authors.lastname: Morris
authors.forename: R W
authors.initials: RW
authors.lastname: Katzberg
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects*; D003287:Contrast Media / Q000008:administration & dosage* / Q000097:blood; D003681:Dehydration / Q000097:blood*; D003974:Diatrizoate Meglumine / Q000008:administration & dosage; D005070:Evans Blue; D005260:Female; D008297:Male; D008353:Mannitol / Q000008:administration & dosage; D009942:Organometallic Compounds; D009994:Osmolar Concentration; D004369:Pentetic Acid; D011817:Rabbits; D013382:Subtraction Technique; D016284:Technetium Tc 99m Pentetate
chemical_list: D003287:Contrast Media; D009942:Organometallic Compounds; D008353:Mannitol; D003974:Diatrizoate Meglumine; D005070:Evans Blue; D004369:Pentetic Acid; D016284:Technetium Tc 99m Pentetate