title: Successful extended hypothermic cardiopulmonary preservation for heart-lung transplantation.
abstract: The inability to obtain sufficiently extended hypothermic organ preservation is a major restriction on clinical heart-lung transplantation. We used core cooling, nonrecirculating retrograde heart perfusion, and lung immersion with liposomal recombinant human superoxide dismutase in an attempt to provide effective 12-hour cardiopulmonary preservation. Donor dogs supported by cardiopulmonary bypass were rapidly cooled to 15 degrees C with cardioplegic arrest, and heterotopic heart and unilateral left lung transplantations were performed. In control dogs (n = 7), hearts and lungs, harvested after core cooling and cardioplegic arrest, were transplanted with a total mean ischemic time of 88 +/- 5 minutes. In group II (n = 7), heart-lung blocks were similarly excised but preserved at 4 degrees C for 12 hours (756 +/- 30 minutes) and then transplanted. During preservation, the lungs were immersed in hyperosmolar extracellular solution. For the heart, retrograde coronary sinus perfusion was performed with intracellular solution containing perfluorochemicals at a temperature of 4 degrees C and a rate of 30 ml/hr for 12 hours. In group III (n = 7), donor organs were similarly excised and preserved for 12 hours (726 +/- 39 minutes), except that liposomal recombinant human superoxide dismutase was administered during harvest, preservation, and reperfusion. Myocardial function, assessed by the ratio of end-systolic pressure to end-systolic dimension, after the 12-hour preservation period in both experimental groups was similar to that of the control group 4 and 6 hours after transplantation. The mean arterial oxygen capacity of the transplanted left lung during ventilation with an inspired oxygen concentration of 40% was also similar in each group. In contrast, the 12-hour preservation of pulmonary function assessed by pulmonary vascular resistance, the accumulation of extravascular lung water, and histologic evidence of alveolar wall injury, interstitial edema, and perivascular hemorrhage were significantly impaired in the absence of liposal recombinant human superoxide dismutase. These findings suggest that successful extended cardiopulmonary preservation for heart-lung transplantation is possible with core cooling, nonrecirculating retrograde heart perfusion, and hypothermic lung immersion incorporating liposomal recombinant human superoxide dismutase.
authors.affiliation: Department of Surgery II, Okayama University Medical School, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Bando
authors.forename: S
authors.initials: S
authors.lastname: Teramoto
authors.forename: M
authors.initials: M
authors.lastname: Tago
authors.forename: H
authors.initials: H
authors.lastname: Teraoka
authors.forename: S
authors.initials: S
authors.lastname: Seno
authors.forename: Y
authors.initials: Y
authors.lastname: Senoo
mesh_terms: D000818:Animals; D002302:Cardiac Output; D003080:Cold Temperature; D004285:Dogs; D006321:Heart / Q000503:physiopathology; D016027:Heart Transplantation; D016041:Heart-Lung Transplantation; D008168:Lung / Q000473:pathology / Q000503:physiopathology; D016040:Lung Transplantation; D009926:Organ Preservation / Q000379:methods*; D010101:Oxygen Consumption; D011994:Recombinant Proteins; D013482:Superoxide Dismutase; D014655:Vascular Resistance
chemical_list: D011994:Recombinant Proteins; D013482:Superoxide Dismutase