Herbimycin A suppresses the reduction of gap-junctional intercellular communication induced by tumor-promoting phorbol ester in 3T3-L1 cells.
We have examined the suppressive effect of herbimycin A on the reduction of gap-junctional intercellular communication that is induced by a tumor-promoting phorbol ester in 3T3-L1 cells. Most cells in growth arrest participated in dye-coupling, as evaluated by the transfer between cells of a fluorescent dye (Lucifer Yellow CH). Treatment of cells with 0.25 microgram/ml herbimycin A slightly enhanced the dye-coupling. This enhancement required treatment for periods as long as 24 h. Addition of 100 ng/ml 12-O-tetradecanoylphorbol-13-acetate (TPA) caused a rapid reduction of dye-coupling. However, addition of TPA did not suppress dye-coupling in cells pretreated for more than 24 h with herbimycin A. Pretreatment of cells for less than 6 h with herbimycin A did not suppress the TPA-induced reduction of dye-coupling. These results suggest that herbimycin A suppresses the reduction of gap-junctional intercellular communication that is induced by TPA through enhancement of the ability of the cells to participate in gap-junctional intercellular communication.
Sasaki
Y
Y
Department of Oral Physiology, Hiroshima University School of Dentistry.
Shiba
Y
Y
Hirono
C
C
Kanno
Y
Y
Takeuchi
T
T
Umezawa
K
K
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016227:Benzoquinones; D002450:Cell Communication / Q000187:drug effects*; D002460:Cell Line / Q000187:drug effects; D004336:Drug Antagonism; D007365:Intercellular Junctions / Q000187:drug effects*; D007546:Isoquinolines; D047029:Lactams, Macrocyclic; D051379:Mice; D011809:Quinones / Q000494:pharmacology*; D017828:Rifabutin / Q000031:analogs & derivatives; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D013997:Time Factors
D000900:Anti-Bacterial Agents; D016227:Benzoquinones; D007546:Isoquinolines; D047029:Lactams, Macrocyclic; D011809:Quinones; D017828:Rifabutin; C020754:herbimycin; C017475:lucifer yellow; D013755:Tetradecanoylphorbol Acetate