title: Identification of Neisseria gonorrhoeae with the ORTHOProbe DNA probe test.
abstract: We evaluated and compared the ORTHOProbe DNA hybridization test (Ortho Diagnostic Systems, Inc., Raritan, NJ) with conventional carbohydrate fermentation and commercial rapid tests for identifying gonococci (GC). The ORTHOProbe system employs a biotinylated DNA probe that hybridizes specifically with chromosomal target sequences of GC. Hybridization is detectable visually following the addition of a streptavidin-peroxidase conjugate in a rapid 10-min. assay. A total of 200 clinical isolates resembling Neisseria (gram-negative, oxidase-positive diplococci) cultured from various sites were tested. The ORTHOProbe test reacted strongly with all GC (94) and not with nongonococcal organisms (106), yielding a sensitivity and specificity of 100%. The ORTHOProbe system provides a convenient and reliable test for identifying Neisseria gonorrhoeae.
authors.affiliation: Molecular Diagnostics Laboratory, Yale-New Haven Hospital, Connecticut.
authors.forename: A P
authors.initials: AP
authors.lastname: Kuritza
authors.forename: S C
authors.initials: SC
authors.lastname: Edberg
authors.forename: C
authors.initials: C
authors.lastname: Chapis
authors.forename: P
authors.initials: P
authors.lastname: Gallo
mesh_terms: D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D009343:Neisseria / Q000235:genetics / Q000302:isolation & purification; D009344:Neisseria gonorrhoeae / Q000235:genetics / Q000302:isolation & purification*; D009345:Neisseria meningitidis / Q000235:genetics / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D011237:Predictive Value of Tests
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial