title: Rapid analysis of sorbitol, galactitol, mannitol and myoinositol mixtures from biological sources.
abstract: The three commonly found hexitols mannitol, sorbitol and galactitol are well separated from each other and from myoinositol by gas chromatography as their butylboronate derivative on Dexsil-400, on a 1:1 mixture of OV-1 and OV-17, or on a DB-17 fused-silica capillary column. The method allows all four substances to be measured by autosampling electron ionization gas chromatography-mass spectrometry (GC-MS) in small tissue samples at organ concentrations as small as 5 mumol/kg wet mass in less than 4 min. Comparisons were made to determine the relative sensitivity of GC-MS and other detection methods. The order of sensitivity was electron ionization GC-MS greater than chemical ionization GC-MS greater than flame photometric detection using a boron-selective filter greater than hydrogen flame ionization detection.
authors.affiliation: Department of Pathology, Washington University School of Medicine, St. Louis, MO 63110.
authors.forename: D M
authors.initials: DM
authors.lastname: Eades
authors.forename: J R
authors.initials: JR
authors.lastname: Williamson
authors.forename: W R
authors.initials: WR
authors.lastname: Sherman
mesh_terms: D000818:Animals; D002849:Chromatography, Gas; D004912:Erythrocytes / Q000032:analysis; D004376:Galactitol / Q000032:analysis* / Q000097:blood; D008401:Gas Chromatography-Mass Spectrometry; D007294:Inositol / Q000032:analysis* / Q000097:blood; D008353:Mannitol / Q000032:analysis* / Q000097:blood; D051381:Rats; D012160:Retina / Q000032:analysis; D012584:Sciatic Nerve / Q000032:analysis; D013012:Sorbitol / Q000032:analysis* / Q000097:blood; D013402:Sugar Alcohols / Q000032:analysis*
chemical_list: D013402:Sugar Alcohols; D004376:Galactitol; D008353:Mannitol; D007294:Inositol; D013012:Sorbitol