[Soy lipoxygenase as the object for the primary testing of potential inhibitors of leukotriene biosynthesis].
The comparative study of the ability of three compounds (indomethacin, agent BW 755 C and arachidonate hydroxamic acid) to inhibit the activity of preparations of soya bean lipoxygenase and to block leukotriene C4 biosynthesis in human leukocytes recorded by reversed phase liquid chromatography was performed. It was shown that irrespectively of purification degree soya bean lipoxygenase is a sufficiently adequate object for primary testing of inhibitors of biosynthesis of leukotrienes with the given type of action. Compounds with IC50 less than or equal to 1.10(-6) mol/l might be considered promising for the further investigation.
Lototskaia
L S
LS
Naĭdenova
I Iu
IIu
Il'iasheva
L M
LM
Poda
G I
GI
Kornilov
A I
AI
D015772:4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine; D001091:Arachidonate Lipoxygenases / Q000037:antagonists & inhibitors*; D001095:Arachidonic Acids / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D004353:Drug Evaluation, Preclinical; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism; D016859:Lipoxygenase Inhibitors; D008213:Lymphocyte Activation / Q000187:drug effects; D011720:Pyrazoles / Q000494:pharmacology; D013189:SRS-A / Q000032:analysis / Q000037:antagonists & inhibitors*; D013025:Glycine max / Q000201:enzymology*; D013056:Spectrophotometry, Ultraviolet
D001095:Arachidonic Acids; D004791:Enzyme Inhibitors; D006877:Hydroxamic Acids; D016859:Lipoxygenase Inhibitors; D011720:Pyrazoles; D013189:SRS-A; D000001:Calcimycin; D015772:4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine; C060260:arachidonate hydroxamic acid; D001091:Arachidonate Lipoxygenases; D007213:Indomethacin
Soevaia lipoksigenaza kak ob''ekt pervichnogo testirovaniia potentsialnykh ingibitorov biosinteza leĭkotrienov.