title: Biosynthesis of tetrahydrobiopterin: a sensitive assay of 6-pyruvoyltetrahydropterin synthase using [2'-3H]dihydroneopterin 3'-triphosphate as substrate.
abstract: Pyruvoyltetrahydropterin synthase catalyzes the release of tritiated water from [2'-3H]dihydroneopterin 3'-triphosphate. The tritiated water formed during the enzymatic reaction is separated from substrate by adsorption of the latter to activated charcoal. The sensitivity and specificity of the assay allows the determination of the enzyme in crude cell extract.
authors.affiliation: Gesellschaft für Strahlen- und Umweltforschung, Institut für Experimentelle Hämatologie, München, F.R.G.
authors.forename: F
authors.initials: F
authors.lastname: Kerler
authors.forename: B
authors.initials: B
authors.lastname: Schwarzkopf
authors.forename: G
authors.initials: G
authors.lastname: Katzenmaier
authors.forename: Q
authors.initials: Q
authors.lastname: Le Van
authors.forename: C
authors.initials: C
authors.lastname: Schmid
authors.forename: I
authors.initials: I
authors.lastname: Ziegler
authors.forename: A
authors.initials: A
authors.lastname: Bacher
mesh_terms: D000327:Adsorption; D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000818:Animals; D001708:Biopterins / Q000031:analogs & derivatives* / Q000096:biosynthesis; D002606:Charcoal; D055598:Chemical Phenomena; D002621:Chemistry; D004492:Edetic Acid / Q000494:pharmacology; D004926:Escherichia coli / Q000201:enzymology*; D008099:Liver / Q000201:enzymology; D008274:Magnesium / Q000494:pharmacology; D051379:Mice; D019798:Neopterin / Q000031:analogs & derivatives; D019761:Phosphorus-Oxygen Lyases; D011621:Pteridines / Q000378:metabolism*; D014316:Tritium
chemical_list: D011621:Pteridines; D014316:Tritium; D002606:Charcoal; C013490:dihydroneopterin triphosphate; D001708:Biopterins; D019798:Neopterin; D004492:Edetic Acid; D000429:Alcohol Oxidoreductases; D019761:Phosphorus-Oxygen Lyases; C043497:6-pyruvoyltetrahydropterin synthase; C003402:sapropterin; D008274:Magnesium