title: Proteins that interact with GTP during sporulation of Bacillus subtilis.
abstract: During sporulation of Bacillus subtilis, several proteins were shown to interact with GTP in specific ways. UV light was used to cross-link [alpha-32P]GTP to proteins in cell extracts at different stages of growth. After electrophoresis, 11 bands of radioactivity were found in vegetative cells, 4 more appeared during sporulation, and only 9 remained in mature spores. Based on the labeling pattern with or without UV light to cross-link either [alpha-32P]GTP or [gamma-32P]GTP, 11 bands of radioactivity were apparent guanine nucleotide-binding proteins, and 5 bands appeared to be phosphorylated and/or guanylated. Similar results were found with Bacillus megaterium. Assuming that GTP might be a type of signal for sporulation, it could interact with and regulate proteins by at least three mechanisms.
authors.affiliation: Department of Biochemistry, University of Illinois, Chicago 60612.
authors.forename: C
authors.initials: C
authors.lastname: Mitchell
authors.forename: J C
authors.initials: JC
authors.lastname: Vary
mesh_terms: D001412:Bacillus subtilis / Q000502:physiology*; D001426:Bacterial Proteins / Q000502:physiology; D019204:GTP-Binding Proteins / Q000502:physiology*; D006160:Guanosine Triphosphate / Q000502:physiology*; D008970:Molecular Weight; D010766:Phosphorylation; D013172:Spores, Fungal
chemical_list: D001426:Bacterial Proteins; D006160:Guanosine Triphosphate; D019204:GTP-Binding Proteins