Prostaglandin production by cultured cynomolgus monkey trabecular meshwork cells.
Monkey trabecular meshwork (MTM) cells synthesize a variety of prostaglandins, including large amounts of prostaglandin E2 (PGE2) and smaller amounts of 6-keto-PGF1 alpha and PGF2 alpha. The predominance of PGE2 production by the MTM cells is similar to that observed in human trabecular meshwork cells. In contrast, the relative amounts of 6-keto-PGF1 alpha and PGF2 alpha were reversed compared with the human cells. The MTM cells produced increased amounts of PGE2 in response to treatment with bradykinin, platelet activating factor, and A-23187. Dexamethasone caused a dose-dependent inhibition of PGE2 production with 50% inhibition by 10(-8) M, although this response was variable.
Weinreb
R N
RN
Department of Ophthalmology, University of California, San Diego, La Jolla 92093.
Mitchell
M D
MD
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis; D000818:Animals; D001920:Bradykinin / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D015237:Dinoprost / Q000096:biosynthesis; D015232:Dinoprostone / Q000096:biosynthesis; D006801:Humans; D008252:Macaca fascicularis; D010972:Platelet Activating Factor / Q000494:pharmacology; D011453:Prostaglandins / Q000096:biosynthesis*; D011863:Radioimmunoassay; D014129:Trabecular Meshwork / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
D010972:Platelet Activating Factor; D011453:Prostaglandins; D000001:Calcimycin; D015121:6-Ketoprostaglandin F1 alpha; D003907:Dexamethasone; D015237:Dinoprost; D015232:Dinoprostone; D001920:Bradykinin