Probing of the canine mammary artery damages endothelium and impairs vasodilation resulting from prostacyclin and endothelium-derived relaxing factor.
It is routine practice for many cardiac surgeons to probe internal mammary arteries to dilate them before their use. The effects of such probing on endothelium integrity, prostacyclin production, and vasodilation resulting from endothelium-derived relaxing factor and from prostacyclin were investigated in vessels isolated from mongrel dogs. Dose-dependent relaxation responses of isolated segments of probed and unprobed mammary arteries to the endothelium-dependent vasodilators methacholine, calcium ionophore (A23187), and melittin were determined in both the presence and absence of indomethacin. Prostacyclin production by probed versus unprobed vascular segments was determined under basal and A23187-stimulated conditions by radioimmunoassay for 6-keto-prostaglandin F1 alpha, and endothelial integrity was determined by scanning electron microscopy. Scanning electron micrographs of segments revealed marked endothelial cell disruption in probed versus unprobed vessels. The dose-dependent relaxation responses to all drugs studied were significantly impaired (p less than 0.05) in probed versus unprobed vessels in both the presence and absence of indomethacin. In addition, prostacyclin release as measured by production of 6-keto-prostaglandin F1 alpha was significantly (p less than 0.05) impaired in probed versus unprobed vessels under both basal and A23187-stimulated conditions. These results imply that routine probing of the internal mammary artery may damage endothelium, impair prostacyclin production, and impair endothelium-dependent vasodilation resulting from both prostacyclin and endothelium-derived relaxing factor.
Johns
R A
RA
Department of Anesthesiology, University of Virginia Medical Center, Charlottesville 22908.
Peach
M J
MJ
Flanagan
T
T
Kron
I L
IL
D000818:Animals; D001685:Biological Factors / Q000502:physiology*; D000001:Calcimycin / Q000494:pharmacology; D004106:Dilatation / Q000379:methods; D004285:Dogs; D004730:Endothelium, Vascular / Q000502:physiology* / Q000648:ultrastructure; D011464:Epoprostenol / Q000096:biosynthesis / Q000502:physiology*; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D008323:Mammary Arteries / Q000293:injuries* / Q000378:metabolism; D008555:Melitten / Q000494:pharmacology; D016210:Methacholine Chloride; D008688:Methacholine Compounds / Q000494:pharmacology; D009569:Nitric Oxide; D009599:Nitroprusside / Q000494:pharmacology; D013895:Thoracic Arteries / Q000293:injuries*; D014664:Vasodilation / Q000187:drug effects
D001685:Biological Factors; D008688:Methacholine Compounds; D016210:Methacholine Chloride; D009599:Nitroprusside; D008555:Melitten; D009569:Nitric Oxide; D000001:Calcimycin; D011464:Epoprostenol; D007213:Indomethacin