Blue-light mediated accumulation of nuclear-encoded transcripts coding for proteins of the thylakoid membrane is absent in the phytochrome-deficient aurea mutant of tomato.
Polyclonal antibodies against pea phytochrome detect 2 protein bands (about 116 and 120 kDa) on blots of crude protein extracts and protein of microsomal preparations of dark-grown tomato seedlings. Both protein bands are undetectable in Western blots of the aurea mutant extracts. Neither protein band is detectable after isogenic wild-type seedlings are illuminated with 3 h of red light, either in the crude extract or in the membrane fraction of the irradiated seedlings; this result is consistent with the hypothesis that both bands are phytochrome. When dark-grown wild-type seedlings are illuminated with 3 h of red light or blue light against a red light background, the transcript levels for chlorophyll a/b-binding proteins of photosystem I and II, plastocyanin, and the subunit II of photosystem I increase. In all cases, the same fluence rate of blue light is much more effective than red light alone, a result that indicates the involvement of a blue/UV-A light photoreceptor in addition to the involvement of the far-red-absorbing form of phytochrome, Pfr. The aurea mutant responds neither to red light nor to blue light. Thus, no Pfr-independent induction of the four transcripts by a blue/UV-A light photoreceptor can be measured in the aurea mutant.
Oelmüller
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Carnegie Institution of Washington, Department of Plant Biology, Stanford, CA 94305.
Kendrick
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Briggs
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WR
D015342:DNA Probes; D007150:Immunohistochemistry; D008027:Light; D045342:Light-Harvesting Protein Complexes; D008565:Membrane Proteins / Q000235:genetics; D009154:Mutation; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics / Q000528:radiation effects; D045331:Photosystem I Protein Complex; D010834:Phytochrome / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics*; D010944:Plants / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects; D012313:RNA / Q000235:genetics; D014158:Transcription, Genetic / Q000528:radiation effects
D015342:DNA Probes; D045342:Light-Harvesting Protein Complexes; D008565:Membrane Proteins; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D010940:Plant Proteins; D010834:Phytochrome; D012313:RNA