Production and immunohistochemical characterization of primary cultures of human embryo brain cells.
Primary cultures of human embryo brain cells (9-12 weeks of pregnancy) were obtained. The use of selective substrates (poly-L-lysine, poly-L-ornithine, reconstructed collagen) made it possible to obtain neuron-rich (up to 80%) cultures viable for no less than 30 days. The highest selectivity with respect to neurons was observed when reconstructed collagen was used. Cell types present in the cultures were identified immunohistochemically using antibodies against glial fibrillary acidic protein, galactocerebroside, fibronectin and neurofilaments. The dynamics of cell growth under different cultivation conditions was studied. Cells in culture have been found to preserve the ability to release neurotransmitters (dopamine, norepinephrine and serotonin). Such primary cultures can serve as a suitable model for studying human brain biochemistry in vitro.
Kondratyev
A D
AD
Institute of Applied Molecular Biology, U.S.S.R. Ministry of Health, Moscow.
Zotova
E E
EE
Loginov
B V
BV
Tsirenina
M L
ML
Melnik
E I
EI
Severin
E S
ES
D001921:Brain / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods; D005865:Gestational Age; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000378:metabolism; D016900:Neurofilament Proteins; D009474:Neurons / Q000166:cytology* / Q000378:metabolism
D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; D016900:Neurofilament Proteins