Comparison of effects of protein kinase C inhibitors on phorbol ester-induced CD4 down-regulation and augmentation of human immunodeficiency virus replication in human T cell lines.
The potent protein kinase C inhibitors staurosporine, H-7, and UCN-01 were investigated for their effects on 12-0-tetradecanoylphorbol-13-acetate (TPA)--mediated CD4 down-regulation and on the augmentation of human immunodeficiency virus (HIV) expression. Staurosporine was the most effective TPA inhibitor for both of these actions. Because of its high cytotoxicity, the effect of H-7 on augmentation of HIV expression could not be determined. UCN-01 had no cytotoxic effect, but caused only little inhibition of the augmentation of HIV expression.
Hamamoto
Y
Y
Department of Virology and Parasitology, Yamaguchi University School of Medicine, Japan.
Hayashida
Y
Y
Kobayashi
S
S
Tamaoki
T
T
Yamamoto
N
N
Kobayashi
N
N
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000470:Alkaloids / Q000494:pharmacology; D015704:CD4 Antigens / Q000276:immunology*; D002460:Cell Line; D015536:Down-Regulation / Q000276:immunology*; D005434:Flow Cytometry; D015683:Gene Products, gag / Q000032:analysis; D006678:HIV / Q000502:physiology*; D015488:HIV Antigens / Q000032:analysis; D016655:HIV Core Protein p24; D006801:Humans; D007546:Isoquinolines / Q000494:pharmacology; D010879:Piperazines / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D019311:Staurosporine / Q000031:analogs & derivatives; D013601:T-Lymphocytes / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity; D014758:Viral Core Proteins / Q000032:analysis; D014779:Virus Replication
D000470:Alkaloids; D015704:CD4 Antigens; D015683:Gene Products, gag; D015488:HIV Antigens; D016655:HIV Core Protein p24; D007546:Isoquinolines; D010879:Piperazines; D014758:Viral Core Proteins; C054852:7-hydroxystaurosporine; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D011493:Protein Kinase C; D019311:Staurosporine; D013755:Tetradecanoylphorbol Acetate