[The reactivity of antigens of Mycoplasma pulmonis derived from mice and rats to naturally infected rat sera].
The reactivity of antigens of 4 mouse and 3 rat derived Mycoplasma pulmonis strains to 20 naturally infected rat sera was studied. The optical density values of the same serum by enzyme-linked immunosorbent assay using the 7 strains as the antigen revealed no marked difference among the strains. M. pulmonis antigens recognized by the antibodies were analyzed by the Western immunoblot method. The antigens with molecular weights of 92 K, 66 K, and 58 K were recognized in the 7 strains at a high frequency.
Terada
E
E
Department of Laboratory Animal Science, School of Hygienic Sciences, Kitasato University, Sagamihara, Japan.
Kobayashi
N
N
Sakurai
Y
Y
Mochida
R
R
Suzuki
H
H
Takakura
A
A
Kagiyama
N
N
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D008970:Molecular Weight; D009177:Mycoplasma pneumoniae / Q000276:immunology*; D011019:Pneumonia, Mycoplasma / Q000276:immunology / Q000662:veterinary*; D051381:Rats; D012376:Rodent Diseases / Q000175:diagnosis* / Q000276:immunology; D012698:Serologic Tests / Q000379:methods
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial