title: Immunisation of cattle against theileriosis in Coast Province, Kenya: laboratory evaluation of a Theileria parva parva stabilate for use in 'infection and treatment' immunisation in the field.
abstract: Theileria parva parva Marikebuni stock, previously shown to give good protection to immunised cattle in Kilifi District, Coast Province of Kenya, was chosen for large scale immunisation in the district. A large sporozoite stabilate was prepared and evaluated for efficacy and safety in the 'infection and treatment' method, using a long or short acting formulation of oxytetracycline. Susceptible cattle were infected with selected doses of stabilate (10(0), 10(-1), 10(-1.7) and left either as untreated controls, or treated with one of the two oxytetracycline formulations. It was concluded that stabilate dilution at 10(-0.7) or 10(-1) in combination with either formulation of oxytetracycline would effect satisfactory immunisation. The short acting oxytetracycline treatment was judged to be the most efficacious in protecting cattle against homologous challenge. On heterologous challenge it was found that T p parva Marikebuni immune cattle were protected against seven T p parva stocks from Kilifi District and also against four stocks of T p parva from other areas of Kenya. In addition, the Marikebuni stock provided partial protection against challenge by T p lawrencei stocks. Furthermore, cattle immune to T p parva and T p lawrencei were protected against lethal challenge of T p parva Marikebuni stock. Thus, it appears that large scale immunisation of cattle against theileriosis in Kilifi District could be undertaken using the Marikebuni stock. With continued assessment, this stock could provide a master theilerial stock for immunisation against cattle theileriosis in areas free of buffaloes elsewhere in Kenya.
authors.affiliation: Protozoology Division, Kenya Agricultural Research Institute, Kikuyu.
authors.forename: J J
authors.initials: JJ
authors.lastname: Mutugi
authors.forename: A S
authors.initials: AS
authors.lastname: Young
authors.forename: A C
authors.initials: AC
authors.lastname: Maritim
authors.forename: S G
authors.initials: SG
authors.lastname: Ndungu
authors.forename: S K
authors.initials: SK
authors.lastname: Mining
authors.forename: A
authors.initials: A
authors.lastname: Linyonyi
authors.forename: P N
authors.initials: PN
authors.lastname: Ngumi
authors.forename: B L
authors.initials: BL
authors.lastname: Leitch
authors.forename: S P
authors.initials: SP
authors.lastname: Morzaria
authors.forename: T T
authors.initials: TT
authors.lastname: Dolan
mesh_terms: D000818:Animals; D016782:Apicomplexa / Q000187:drug effects / Q000276:immunology*; D002417:Cattle; D007114:Immunization / Q000662:veterinary*; D007630:Kenya; D008297:Male; D010118:Oxytetracycline / Q000494:pharmacology / Q000627:therapeutic use*; D013801:Theileriasis / Q000188:drug therapy / Q000517:prevention & control*
chemical_list: D010118:Oxytetracycline