Comparative study of human M2-type pyruvate kinases isolated from human leukocytes and erythrocytes of a patient with red cell pyruvate kinase hyperactivity.
M2-type pyruvate kinases (M2-PK) have been isolated from human leukocytes and from the erythrocytes of a patient with erythrocyte PK hyperactivity. The kinetic characteristics of the patient erythrocyte M2-PK were similar to those of leukocyte M2-PK except for the Hill coefficient of phosphoenol pyruvate kinetics that showed little difference in the values. The patient erythrocyte M2-PK displayed complete immunological identity with leukocyte M2-PK in immunodiffusion, immunoblotting and immunoneutralization. The sensitivity to proteolysis by trypsin and the electrophoretic migration in different conditions were similar for the M2-PK of both origins. These results suggest an identity between this M2-PK abnormally present in erythrocytes and the M2-PK from leukocytes.
Kechemir
D
D
Inserm U-91, Hôpital Henri-Mondor, Créteil, France.
Max-Audit
I
I
Rosa
R
R
D002852:Chromatography, Ion Exchange; D004795:Enzyme Stability; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D015151:Immunoblotting; D005779:Immunodiffusion; D007527:Isoenzymes / Q000097:blood* / Q000302:isolation & purification; D007700:Kinetics; D007962:Leukocytes / Q000201:enzymology*; D008970:Molecular Weight; D011770:Pyruvate Kinase / Q000097:blood* / Q000302:isolation & purification
D007527:Isoenzymes; D011770:Pyruvate Kinase