Complement binding on serum-sensitive and serum-resistant transformants of Neisseria gonorrhoeae: effect of presensitization with a non-bactericidal monoclonal antibody.
The binding of C3 and C9 on serum sensitive (FA635) and serum resistant (FA638) transformants of serum sensitive Neisseria gonorrhoeae strain F62 was examined. Previous studies showed that these transformants have Protein IAs which are minimally different by proteinase K cleavage and primary structural and peptide mapping and bear LPS which vary slightly on SDS-PAGE. Binding of C3 and C9 on FA635 exceeded binding on FA638 in NHS and in adsorbed NHS. Monoclonal antibody 4G5, which binds to PI on FA638 but not FA635, increases C9 binding on FA638 to levels 3-3.5 fold greater than on FA635 but does not result in killing. The majority of additional 125IC9 deposited on FA638 following presensitization with 4G5 is released from the bacterial surface by trypsin. These results extend our earlier results with N. gonorrhoeae by showing that, although PI monoclonals can lead to substantial deposition of non-bactericidal C5b-9, this C5b-9 is not fully inserted into the gonococcal outer membrane.
Joiner
K A
KA
Laboratory of Clinical Investigation, NIH, Bethesda, MD 20892.
Puentes
S M
SM
Warren
K A
KA
Scales
R A
RA
Judd
R C
RC
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D015153:Blotting, Western; D003176:Complement C3 / Q000276:immunology; D003186:Complement C9 / Q000276:immunology; D003165:Complement System Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D005798:Genes, Bacterial; D007114:Immunization; D007700:Kinetics; D009344:Neisseria gonorrhoeae / Q000235:genetics / Q000276:immunology*; D018272:Porins; D011485:Protein Binding; D014169:Transformation, Bacterial
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins; D003176:Complement C3; D003186:Complement C9; D018272:Porins; C057699:porin protein, Neisseria; D003165:Complement System Proteins