[Characteristics of the monoclonal antibodies induced by the Crimean hemorrhagic fever virus].
Twelve lines of hybridomas secreting monoclonal antibodies (MCA) have been generated by fusion of spleen lymphocytes of BALB/c mice immunized with crude Crimean hemorrhagic fever virus (CHF). The hybridomas multiply actively in vitro (over 50 passages) and as ascitic tumors in the abdominal cavity of BALB/c mice. MCA were characterized by indirect immunofluorescence (IF), complement fixation (CF), biological neutralization test (NT), agar gel diffuse precipitation tests, and by the type of immunoglobulins. In the indirect IF, all kinds of MCA reacted with CHF virus, in CF only GEMA 12 and GEMA 31 which also precipitated the virus antigen in AGDPT. The CF-positive MCA belonged to the IgG2a and IgG2b subclasses, but 2 more MCA species of the same immunoglobulin type did not react in CF. No neutralizing properties have been found with MCA. All MCA reacted similarly in indirect IF and CF both with CHF and Congo viruses. Among other members of the CHF-Congo antigenic group, only GEMA 4 MCA interacted with Hazara virus. MCA generated in this study are intended for use in identification of CHF and Congo viruses in diagnostic tests.
Gaĭdamovich
S Ia
SIa
Mel'nikova
E E
EE
Shutkova
T M
TM
Novokhatskiĭ
A S
AS
Turchinskaia
A L
AL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis*; D000914:Antibodies, Viral / Q000032:analysis / Q000096:biosynthesis*; D001202:Ascitic Fluid / Q000276:immunology; D002043:Bunyaviridae / Q000276:immunology*; D002460:Cell Line; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D007117:Immunization, Secondary; D007136:Immunoglobulins / Q000032:analysis; D007158:Immunologic Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D007136:Immunoglobulins
Kharakteristika monoklonal'nykh antitel, indutsirovannykh virusom krymskoĭ gemorragicheskoĭ likhoradki.