Inactivation and elimination of viruses during the fractionation of an intravenous immunoglobulin preparation: liquid heat treatment and polyethylene glycol fractionation.
A method for the heat treatment of human IgG solution at 60 degrees C for 10 h was established. Human immunodeficiency, mumps, vaccinia and 4 other viruses were added to the IgG solution in 33% sorbitol and heated at 60 degrees C. Those viruses were inactivated within 1 h. Heat-treated intravenous IgG (IVIG-H) was prepared by heat treatment and polyethylene glycol (PEG) fractionation. Conventional nonheated intravenous IgG (IVIG-C) was prepared from the same source paste by the fractionation method. No physicochemical or biological difference was observed between the heated and control IVIG preparations.
Uemura
Y
Y
Department of Research and Development, Green Cross Corporation, Osaka, Japan.
Uriyu
K
K
Hirao
Y
Y
Takechi
K
K
Ishikawa
H
H
Nakajima
T
T
Kagitani
Y
Y
Yokoyama
K
K
Funakoshi
S
S
Nishida
M
M
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000936:Antigen-Antibody Complex; D002478:Cells, Cultured; D005591:Chemical Fractionation; D002642:Chick Embryo; D002850:Chromatography, Gel; D003165:Complement System Proteins / Q000276:immunology; D004168:Diphtheria Toxoid / Q000276:immunology; D004588:Electrophoresis, Cellulose Acetate; D006358:Hot Temperature; D006801:Humans; D007074:Immunoglobulin G / Q000008:administration & dosage* / Q000276:immunology; D010587:Phagocytosis / Q000187:drug effects; D011092:Polyethylene Glycols; D011550:Pseudomonas aeruginosa; D013012:Sorbitol; D013056:Spectrophotometry, Ultraviolet; D014407:Tumor Cells, Cultured; D014780:Viruses / Q000302:isolation & purification*
D000906:Antibodies; D000936:Antigen-Antibody Complex; D004168:Diphtheria Toxoid; D007074:Immunoglobulin G; D011092:Polyethylene Glycols; D013012:Sorbitol; D003165:Complement System Proteins