Structural homology of interferon-gamma with B-cell growth factor and its proliferative effect on long term B-cell lines.
In this study, we have demonstrated that recombinant human interferon-gamma (IFN-gamma) acts as a B cell growth factor (BCGF) for long term B cell lines. Immuno dot blot analysis with polyclonal goat antibody against homogenously purified 60K intracellular BCGF (IC-BCGF) was shown to recognize IFN-gamma and IFN-alpha. Out of three E. coli derived recombinant IFN-gamma molecules tested, two containing the complete C-terminal were reactive with antibody and also exhibited BCGF activity, while the third lacking 15 amino acids from C-terminal did not exhibit BCGF activity nor any reaction to anti-IC-BCGF antibodies in immuno dot blot analysis. The proliferative effect of IFN-gamma on long term B cells could be blocked by anti-IC-BCGF antibodies in a dose dependent manner. These results indicated that this BCGF activity may be a result of structural homology between BCGF and C-terminal peptide of IFN-gamma.
Sekhsaria
S
S
Department of Molecular Pathology, University of Texas, M.D. Anderson Cancer Center, Houston 77030.
Sahasrabuddhe
C G
CG
D000917:Antibody Formation; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology; D001681:Biological Assay; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D006801:Humans; D015151:Immunoblotting; D007371:Interferon-gamma / Q000032:analysis*; D015847:Interleukin-4; D007378:Interleukins / Q000032:analysis*; D010641:Phenotype; D011994:Recombinant Proteins
D007378:Interleukins; D011994:Recombinant Proteins; D015847:Interleukin-4; D007371:Interferon-gamma