Assessment of phagocytic activity of granulocytes using laser flow cytometry.
A laser flow cytometer assay for assessing two functions of polymorphonuclear leucocytes is described. The technique permits the quantitation of phagocytosis and intracellular killing of yeast cells by leucocytes and is illustrated with results obtained using porcine polymorphonuclear leucocytes.
Buschmann
H
H
Institute for Medical Microbiology and Infectious Medicine, Veterinary Faculty, University of Munich, F.R.G.
Winter
M
M
D000818:Animals; D002469:Cell Separation; D005434:Flow Cytometry; D006098:Granulocytes / Q000502:physiology*; D007834:Lasers; D010587:Phagocytosis; D012441:Saccharomyces cerevisiae; D013552:Swine