Pancreatic islet-specific T-cell clones from nonobese diabetic mice.
We have produced a panel of islet-specific T-cell clones from nonobese diabetic (NOD) mice. These clones proliferate and make interleukin 2 in an antigen-specific manner in response to NOD antigen-presenting cells and islet cells. Most of the clones respond to islet-cell antigen from different mouse strains but only in the presence of antigen-presenting cells bearing the class II major histocompatibility complex of the NOD mouse. In vivo, the clones mediate the destruction of islet, but not pituitary, grafts. Furthermore, pancreatic sections from a disease transfer experiment with one of the clones showed a pronounced cellular infiltration and degranulation of islets in nondiabetic (CBA x NOD)F1 recipients.
Haskins
K
K
Barbara Davis Center for Childhood Diabetes, University of Colorado Health Sciences Center, Denver 80262.
Portas
M
M
Bergman
B
B
Lafferty
K
K
Bradley
B
B
D000818:Animals; D002460:Cell Line; D002999:Clone Cells; D003921:Diabetes Mellitus, Experimental / Q000276:immunology* / Q000601:surgery; D007515:Islets of Langerhans / Q000276:immunology*; D016381:Islets of Langerhans Transplantation; D008213:Lymphocyte Activation; D051379:Mice; D008817:Mice, Mutant Strains; D010902:Pituitary Gland / Q000637:transplantation; D013601:T-Lymphocytes / Q000276:immunology*