Peroxidative events in stored platelet concentrates.
Changes in metabolic and functional activity of platelets stored as platelet concentrates in plastic bags highly permeable to gases were investigated. The following parameters were measured daily: pH, pO2, pCO2, HCO3, glucose, lactic acid, lactic dehydrogenase, cellular ATP and platelet aggregation induced by different agents (collagen and ADP). As indexes of lipid peroxidative damage, the cellular levels of conjugated dienes, malonyldialdehyde and some antioxidant molecules such as coenzyme Q10 and vitamin E were determined. A marked increase in pO2, conjugated dienes, malonyldialdehyde, lactic acid and lactic dehydrogenase activity was observed during the preservation. Platelet ATP content was unmodified and a remarkable decrease in platelet aggregability was found. pCO2, cyclooxygenase activity, vitamin E, coenzyme Q10, bicarbonate and glucose showed a rapid fall. Our data seem to indicate a preservation of platelet metabolic activity and a correlation between increased lipid peroxidation and functional impairement.
Fagiolo
E
E
Blood Transfusion and Immunohematology Service, Catholic University S. Cuore, Rome, Italy.
Lippa
S
S
Mores
N
N
Oradei
A
A
Aureli
V
V
D000225:Adenine; D000255:Adenosine Triphosphate / Q000097:blood; D001639:Bicarbonates / Q000097:blood; D001792:Blood Platelets / Q000378:metabolism*; D001793:Blood Preservation; D002245:Carbon Dioxide / Q000097:blood; D002951:Citrates; D005947:Glucose; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007770:L-Lactate Dehydrogenase / Q000097:blood; D007773:Lactates / Q000097:blood; D019344:Lactic Acid; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000097:blood; D010100:Oxygen / Q000097:blood; D010545:Peroxides / Q000097:blood*; D010710:Phosphates; D010974:Platelet Aggregation; D014451:Ubiquinone / Q000097:blood; D014810:Vitamin E / Q000097:blood
D001639:Bicarbonates; C034876:CPDA solutions; D002951:Citrates; D007773:Lactates; D010545:Peroxides; D010710:Phosphates; D014451:Ubiquinone; D014810:Vitamin E; D002245:Carbon Dioxide; D019344:Lactic Acid; D008315:Malondialdehyde; D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase; D005947:Glucose; D000225:Adenine; D010100:Oxygen