Expression of the trpC gene from Penicillium chrysogenum in Aspergillus nidulans.
The heterologous expression in Aspergillus nidulans of a gene involved in tryptophan biosynthesis from Penicillium chrysogenum is described. With the chimeric plasmid pPC-31, which carries the cloned trpC gene, approximately 10-40 "stable" transformants per microgram of DNA were obtained, with selection for complementation of the mutant allele. This frequency was increased 10-fold by the insertion of the ans1 fragment into the transformation vector. Southern hybridization analysis revealed that transformation occurred as a consequence of the integration of vector sequences into the host chromosome at a variety of sites within the genome.
Picknett
T M
TM
School of Biotechnology, Polytechnic of Central London, United Kingdom.
Saunders
G
G
Holt
G
G
D001233:Aspergillus nidulans / Q000235:genetics*; D015139:Blotting, Southern; D003470:Culture Media; D015870:Gene Expression; D005800:Genes, Fungal; D009693:Nucleic Acid Hybridization; D010407:Penicillium / Q000235:genetics*; D010408:Penicillium chrysogenum / Q000235:genetics*; D010641:Phenotype; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D014170:Transformation, Genetic; D014364:Tryptophan / Q000378:metabolism
D003470:Culture Media; D014364:Tryptophan