Flow microcalorimetric assay of antibiotics--III. Zinc bacitracin and its combinations with polymyxin B sulphate and neomycin sulphate on interaction with Micrococcus luteus.
A flow microcalorimetric assay for zinc bacitracin has been developed which has better reproducibility (relative standard deviation less than 2%) and sensitivity (0.02 micrograms ml-1) than conventional microbiological assays, and requires an assay time of between 7.5-9 h. The assay is not suitable for zinc bacitracin determinations in the presence of equimolar concentrations of polymyxin B sulphate or neomycin sulphate, or of these antibiotics in the proportions in which they occur in the commercial preparation Trisep (ICI, Macclesfield, UK).
Joslin Kjeldsen
N
N
Department of Chemistry, Chelsea College, University of London, UK.
Beezer
A E
AE
Miles
R J
RJ
D001414:Bacitracin / Q000032:analysis* / Q000494:pharmacology; D001681:Biological Assay; D002151:Calorimetry / Q000379:methods; D004359:Drug Therapy, Combination / Q000032:analysis / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D008837:Micrococcus / Q000187:drug effects*; D009355:Neomycin / Q000032:analysis* / Q000494:pharmacology; D011112:Polymyxin B / Q000032:analysis* / Q000494:pharmacology
D001414:Bacitracin; D009355:Neomycin; D011112:Polymyxin B