Urokinase-type plasminogen activator in endothelial cells during acute inflammation of the appendix.
Urokinase and tissue-type plasminogen activators (u-PA and t-PA) were identified immunohistochemically in normal and inflamed human appendices by means of polyclonal and monoclonal antibodies. In addition, extracts of the tissues were analyzed for u-PA and t-PA by ELISA. Twelve appendices (five normal and seven with acute inflammation) were analyzed. In the normal appendices, there was a strong staining of the endothelial cells for t-PA, whereas there was negative staining for u-PA. In contrast, the endothelial cells in the inflamed appendices showed u-PA immunoreactivity, and negative or very weak reactions for t-PA. In the inflamed appendix, there was also a labeling of u-PA in fibroblast-like cells and in interstitial areas. The specificity of the staining was supported by a variety of staining controls and also by analysis of tissue extracts with ELISA, showing that on the average the inflamed appendices contained more than twice as much mu-PA per mg of protein as the normal appendices and less than one third of the amount of t-PA.
Grøndahl-Hansen
J
J
Department of Biochemistry and Nutrition Technical University of Denmark, Lyngby.
Kirkeby
L T
LT
Ralfkiaer
E
E
Kristensen
P
P
Lund
L R
LR
Danø
K
K
D001064:Appendicitis / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D004727:Endothelium / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D005343:Fibrinolytic Agents / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D010960:Plasminogen Activators / Q000276:immunology / Q000378:metabolism*; D010959:Tissue Plasminogen Activator / Q000276:immunology / Q000378:metabolism; D014568:Urokinase-Type Plasminogen Activator / Q000276:immunology / Q000378:metabolism*
D005343:Fibrinolytic Agents; D010960:Plasminogen Activators; D010959:Tissue Plasminogen Activator; D014568:Urokinase-Type Plasminogen Activator