Scatchard analysis of EGF receptor and effects of EGF on growth and TA-4 production of newly established uterine cervical cancer cell line (OMC-1).
Effects of epidermal growth factor (EGF) on growth and tumor antigen-4 (TA-4) production of newly established uterine cervical cancer cell line (OMC-1) are reported. OMC -1 was established from a metastatic lesion of Virchow's lymph node of a large cell non-keratinizing squamous cell carcinoma of the uterine cervix and successively subcultured for about 4 years. Scatchard analysis of EGF binding to OMC-1 cells indicated a single class of binding sites with a dissociation constant (Kd) of 360 pM. The theoretical maximum number of binding sites was 2.4 x 10(4) sites/cell. The growth of OMC-1 cells was stimulated by EGF at 0.01-0.1 nM and inhibited at higher concentrations. The TA-4 production of OMC-1 cells was slightly stimulated by EGF at 0.01-1 nM and significantly stimulated at 10 nM. OMC-1 may serve as one of the available model systems for studies of regulation of proliferation and tumor marker production by EGF, particularly in cervical squamous cell carcinomas.
Ueda
M
M
Department of Obstetrics and Gynecology, Osaka Medical College.
Ueki
M
M
Yamada
T
T
Okamoto
Y
Y
Maeda
T
T
Sugimoto
O
O
Otsuki
Y
Y
D000951:Antigens, Neoplasm / Q000096:biosynthesis; D002294:Carcinoma, Squamous Cell / Q000276:immunology / Q000473:pathology*; D004815:Epidermal Growth Factor / Q000502:physiology*; D066246:ErbB Receptors / Q000378:metabolism*; D005260:Female; D006801:Humans; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000276:immunology / Q000473:pathology*
D000951:Antigens, Neoplasm; C036649:tumor antigen TA-4, human; D004815:Epidermal Growth Factor; D066246:ErbB Receptors