The effect of lead on protein and DNA metabolism of normal and lead-adapted rat kidney cells in culture.
Cultures of epithelial rat kidney cells were used to investigate the effect of lead on protein and DNA metabolism. Lead-adapted cells were cultured in 5 and 10 microM Pb(NO3)2 for several months and subsequently showed the same growth characteristics but a higher resistance to increased lead doses than untreated cells. Both normal and adapted cell types were exposed to lead nitrate in concentrations from 5 to 1000 microM for incubation periods of from 1 to 24 h. 3H-leucine incorporation was increased in 10 microM lead by up to 140% in normal but not in adapted cells, pointing to a resistance forming mechanism. Not only is the overall rate of protein synthesis increased, but separations of cytosolic proteins by SDS-polyacrylamide electrophoresis indicate the novel or increased synthesis of several proteins. A difference could also be observed in the DNA metabolism, where normal cells show a very pronounced increase of up to 900% after 24 h of incubation. This was not observed in adapted cells.
Hitzfeld
B
B
Kernforschungszentrum Karlsruhe, Institut für Genetik und Toxikologie, FRG.
Planas-Bohne
F
F
Taylor
D
D
D000222:Adaptation, Physiological / Q000187:drug effects; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D007854:Lead / Q000494:pharmacology*; D007930:Leucine / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D051381:Rats
D011506:Proteins; D007854:Lead; D004247:DNA; D007930:Leucine