title: The Drosophila proteasome undergoes changes in its subunit pattern during development.
abstract: The two-dimensional electrophoretic protein subunit pattern of the proteasome, which is a mulifunctional non-lysosomal proteinase, was analyzed throughout the development of Drosophila melanogaster. The experiments show that the proteasome is already present in early embryos and its characteristic gross morphology as judged by the outer diameter of 12 nm and the inner depression of 3 nm remains unaltered. The electrophoretic analysis of the enzyme subunits demonstrates that the proteasome undergoes, dependent on development, alterations in its protein composition. The most simple subunit pattern is observed in Schneider's S-3 tissue culture cells and early embryos while with ongoing fly development the subunit pattern of the proteasome becomes increasingly complex. 32P-Labeling and immunoblotting experiments indicate that post-translational modification of the subunits must in part be responsible for the development-dependent diversification of the subunit pattern. Our data raise the possibility that the in vivo proteolytic activity and the in vivo substrate specificity of the proteasome may be regulated by modification of its subunit composition during fly development.
authors.affiliation: Molekulare Genetik/ZMBH, University of Heidelberg, Federal Republic of Germany.
authors.forename: C
authors.initials: C
authors.lastname: Haass
authors.forename: P M
authors.initials: PM
authors.lastname: Kloetzel
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster / Q000196:embryology / Q000201:enzymology* / Q000254:growth & development; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000032:analysis* / Q000302:isolation & purification; D007814:Larva / Q000201:enzymology; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D009097:Multienzyme Complexes / Q000032:analysis*; D011679:Pupa / Q000201:enzymology
chemical_list: D046911:Macromolecular Substances; D009097:Multienzyme Complexes; D010450:Endopeptidases