Morphology of supravital brain slices pre-incubated in a physiological solution prior to fixation.
Slices of 400 microns thickness were prepared from various regions of the adult rat brain using a vibratome, and incubated in oxygenated Krebs-Henseleit medium for 3 h prior to processing for light and electron microscopy. Previous findings in cerebellar slices suggest that the structural recovery requires at least a 1 h period of incubation. In the present study, vibratome-cut slices of other brain areas such as the hippocampus, parietal cortex, striatum and median eminence were shown to display after this period a gross and fine structure comparable to in situ fixed tissues, and to retain it for at least 3 h of incubation. These areas are thought to be promising for further in vitro functional morphological experimentation.
Hajós
F
F
1st Department of Anatomy, Semmelweis University Medical School, Budapest, Hungary.
Garthwaite
J
J
Garthwaite
G
G
Csillag
A
A
D000818:Animals; D001921:Brain / Q000033:anatomy & histology* / Q000648:ultrastructure; D002531:Cerebellum / Q000033:anatomy & histology; D002540:Cerebral Cortex / Q000033:anatomy & histology; D003342:Corpus Striatum / Q000033:anatomy & histology; D005260:Female; D005404:Fixatives; D006624:Hippocampus / Q000033:anatomy & histology; D007031:Hypothalamus / Q000033:anatomy & histology; D066298:In Vitro Techniques; D008297:Male; D008526:Medulla Oblongata / Q000033:anatomy & histology; D008636:Mesencephalon / Q000033:anatomy & histology; D009445:Neuroanatomy / Q000379:methods*; D011149:Pons / Q000033:anatomy & histology; D051381:Rats; D014021:Tissue Preservation / Q000379:methods*
D005404:Fixatives