[Changes in poly (ADP-ribose) polymerase activity in post-hepatectomized rat liver nuclei with glucagon and insulin].
To elucidate the effect of combined therapy of glucagon and insulin on the hepatectomized remnant liver of rats, postoperative changes of liver nuclear poly (ADP-ribose) polymerase activity were measured. The maximal value of poly (ADP-ribose) polymerase activity without glucagon and insulin was revealed as 354 +/- 44pmole/mg/min at the stage of 5 days after partial hepatectomy. In contrast, the enzyme activity with glucagon and insulin reached to peak value as 242 +/- 46pmole/mg/min at the stage of 2 days after hepatectomy. Liver weight regeneration rate of the group with glucagon and insulin showed higher values than that of the group without glucagon and insulin in the period from 5 to 21 days after hepatectomy. The amounts of DNA showed similar changes to poly (ADP-ribose) polymerase activity after hepatectomy. Thus it is suggested that the combined therapy of glucagon and insulin may act to promote liver regeneration through the changes of poly (ADP-ribose) polymerase activity.
Fujii
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Y
Second Department of Surgery, Kinki University School of Medicine, Osakasayama, Japan.
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000201:enzymology; D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA / Q000378:metabolism; D004359:Drug Therapy, Combination; D005934:Glucagon / Q000008:administration & dosage / Q000627:therapeutic use*; D006498:Hepatectomy; D007328:Insulin / Q000008:administration & dosage / Q000627:therapeutic use*; D007657:Ketone Bodies / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008115:Liver Regeneration / Q000187:drug effects; D008297:Male; D009929:Organ Size / Q000187:drug effects; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D011184:Postoperative Period; D051381:Rats; D011919:Rats, Inbred Strains
D007328:Insulin; D007657:Ketone Bodies; D004247:DNA; D005934:Glucagon; D011065:Poly(ADP-ribose) Polymerases