Contribution of guanine nucleotide regulatory proteins to prostaglandin hyperalgesia in the rat.
The contribution of the stimulatory guanine nucleotide regulatory protein (Gs) to prostaglandin E2 (PGE2)-induced hyperalgesia was investigated in the hairy skin of the rat hindpaw using the Randall-Selitto paw-withdrawal test. Although without effect alone, guanosine-5'-[gamma-thio]triphosphate (GTP gamma S) and cholera toxin--which activate Gs--both increased, while guanosine-5'-[beta-thio] diphosphate (GDP beta S)--which prevents the activation of Gs--decreased the hyperalgesia induced by PGE2. These data support the hypothesis that the action of PGE2 on primary afferent nociceptors leading to decreases in paw-withdrawal threshold is Gs-mediated.
Taiwo
Y O
YO
Department of Medicine, University of California, San Francisco 94143.
Levine
J D
JD
D000818:Animals; D002772:Cholera Toxin / Q000494:pharmacology; D004347:Drug Interactions; D019204:GTP-Binding Proteins / Q000494:pharmacology* / Q000502:physiology; D006930:Hyperalgesia / Q000139:chemically induced* / Q000378:metabolism / Q000503:physiopathology; D006941:Hyperesthesia / Q000139:chemically induced*; D008297:Male; D009475:Neurons, Afferent / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology*; D010146:Pain / Q000378:metabolism; D011458:Prostaglandins E / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012684:Sensory Thresholds / Q000187:drug effects
D011458:Prostaglandins E; D002772:Cholera Toxin; D019204:GTP-Binding Proteins