Dot-enzyme immunoassay for visual detection of Brucella melitensis antibodies.
An indirect enzyme-linked immunosorbent assay, using antigen coupled to paper, has been adapted for the detection of Brucella melitensis antibodies. Optimum conditions were achieved by incubation of 1 ml of diluted serum with a single piece of paper coated with purified Brucella antigens for a period of one hour, and by addition of a goat anti-human enzyme conjugate antibody for one hour again. Under these conditions 80 human sera were examined and the results obtained were compared with Wright agglutination test.
Leonardi
M S
MS
Institute of Microbiology, Faculty of Medicine and Surgery, University of Messina, Italy.
Zummo
S
S
Gazzara
D
D
D000372:Agglutination Tests; D000907:Antibodies, Bacterial / Q000032:analysis*; D002002:Brucella / Q000276:immunology*; D002006:Brucellosis / Q000175:diagnosis* / Q000382:microbiology; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male
D000907:Antibodies, Bacterial