Some characteristics of aggregates of IgG and plasma proteins in heat-treated factor VIII concentrates.
Eight batches of commercial heat-treated and one untreated factor VIII concentrate from 6 producers were analyzed for their content of IgG, IgG subclasses, IgG aggregates and the presence of other plasma proteins combined with the IgG as well as for anticomplement activity. Methods used were thin-layer gel filtration, immuno-gel filtration, spot immuno-precipitate assay in a double antibody version and an agarose plate haemolysis inhibition assay of complement fixation. The IgG content varied from 0.1-6.90 g/l. In all preparations IgG existed as monomers and aggregates. Associated with the IgG were also found, at significantly increased amounts compared to normal serum and intravenous immunoglobulin, one to four of the following plasma proteins; fibronectin, fibrinogen, von Willebrand factor antigen, Clq, albumin and IgA. Three batches from two producers had high anticomplementary activity, presumably caused by the IgG aggregates. Two of these deviated strikingly from normal human serum pools in percent distribution of IgG subclasses. It is hypothesized that these aggregates can induce side effects or cause immunological aberrations.
Wadsworth
C
C
Department of Clinical Immunology, University of Göteborg, Sweden.
Hanson
L A
LA
Kjellman
H
H
Söderström
T
T
Blombäck
M
M
D001798:Blood Proteins / Q000302:isolation & purification*; D002855:Chromatography, Thin Layer; D003165:Complement System Proteins / Q000276:immunology; D005169:Factor VIII / Q000032:analysis*; D006358:Hot Temperature; D006801:Humans; D007074:Immunoglobulin G / Q000145:classification / Q000302:isolation & purification* / Q000502:physiology; D046911:Macromolecular Substances; D011233:Precipitin Tests
D001798:Blood Proteins; D007074:Immunoglobulin G; D046911:Macromolecular Substances; D005169:Factor VIII; D003165:Complement System Proteins