The pentose cycle (hexose monophosphate shunt). Rigorous evaluation of limits to the flux from glucose using 14CO2 data, with applications to peripheral ganglia of chicken embryos.
The difference between the 14CO2 outputs from [1-14C]glucose and [6-14C]glucose has frequently been used as a measure of activity in the hexose monophosphate shunt without considering the exact significance of this difference. Assuming only 1) that all C-1 of glucose is released to CO2 on entry to the shunt and 2) that the shunt provides the only mechanism for increasing C-1 of glucose over C-6 of glucose in CO2, it is very simply shown that the flux from glucose to the shunt is not less than the difference between the 14CO2 outputs at any time after adding labeled glucose nor more than the steady-state output of 14CO2 from [1-14C]glucose. Moreover, absence of a 14CO2 difference does not prove that the shunt is absent or inactive. The value for the minimum flux rate can be maximized by following the time course of the C-1 - C-6 difference in 14CO2 during the transient phase before isotopic equilibration is complete, but useful values can be obtained when the time course is not available. The above relationships are applicable to gluconeogenic as well as non-gluconeogenic tissues. Applications of these relationships to peripheral ganglia from chicken embryos, in which the 14CO2 difference passes through a maximum during incubation, show that 27-37% of the glucose taken up enters the pentose cycle in sympathetic ganglia from 10-day-old embryos, while 17-36% enters the cycle in 15-day-old dorsal root ganglia.
Larrabee
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Thomas C. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, Maryland 21218.
D000818:Animals; D002245:Carbon Dioxide / Q000032:analysis; D002250:Carbon Radioisotopes; D002642:Chick Embryo; D002952:Citric Acid Cycle; D005727:Ganglia, Spinal / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D007700:Kinetics; D008962:Models, Theoretical; D010427:Pentose Phosphate Pathway; D011865:Radioisotope Dilution Technique
D002250:Carbon Radioisotopes; D002245:Carbon Dioxide; D005947:Glucose