title: Nucleotide sequence of the right early region of Bacillus phage phi 15 and comparison with related phages: reorganization of gene 17 during evolution.
abstract: The rightmost 2016 bp of the Bacillus subtilis phage phi 15 genome were sequenced. The nucleotide sequence was compared with the homologous regions of the related phages PZA and phi 29. There are six open reading frames (ORFs) in this region of the phi 15 genome; all of them are present in the PZA and phi 29 genomes. One of the ORFs was assigned to gene 17, which is involved in the replication of the phage DNA. Gene 17 has undergone reorganization during the evolution of this phage family. Comparison of the nucleotide sequence of its mRNA-like strand in phi 15, PZA and phi 29 showed that deletions in its central and 3'-end-proximal parts are tolerated and do not interfere with the gene 17 product function. It seems that the only portion of gene 17 that has to be conserved to encode the functional product is its 5'-end-proximal part.
authors.affiliation: Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Prague.
authors.forename: V
authors.initials: V
authors.lastname: Benes
authors.forename: L
authors.initials: L
authors.lastname: Arnold
authors.forename: J
authors.initials: J
authors.lastname: Smrt
authors.forename: V
authors.initials: V
authors.lastname: Paces
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics; D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D005075:Biological Evolution; D004279:DNA, Viral; D015321:Gene Rearrangement; D005814:Genes, Viral; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004279:DNA, Viral