Immune induction of major histocompatibility complex antigens on a human thyroid cell line (SGHTL-34).
Expression of major histocompatibility complex antigens by epithelial cells may play a role in the aetiology of autoimmune disorders. We have studied the effect of gamma-interferon on SGHTL-34, a human thyroid cell line which constitutively expresses class I but not class II antigens. gamma-Interferon induced the expression of class II and increased the expression of class I molecules (assessed by flow cytofluorimetry) in a dose-dependent manner. Thyrotrophin or phytohaemagglutinin had no effect on either class I or class II expression. However, a supernatant from phytohaemagglutinin-stimulated peripheral blood mononuclear cells, containing 6400 U gamma-interferon/ml, was an effective inducer of both class I and class II antigens. These data clarify earlier studies using primary thyroid cultures, which are contaminated with cells of the immune system.
King
S L
SL
Department of Immunology, St George's Hospital Medical School, London.
Whitley
G S
GS
Page
S
S
Nussey
S S
SS
Johnstone
A P
AP
D002460:Cell Line; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000187:drug effects; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology; D013268:Stimulation, Chemical; D013954:Thyroglobulin / Q000494:pharmacology; D013961:Thyroid Gland / Q000166:cytology / Q000276:immunology*
D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D010835:Phytohemagglutinins; D011994:Recombinant Proteins; D007371:Interferon-gamma; D013954:Thyroglobulin