title: Detection of Mycoplasma pneumoniae in simulated clinical samples by polymerase chain reaction. Brief report.
abstract: Polymerase chain reaction (PCR) was used to detect Mycoplasma (M) pneumoniae DNA in simulated clinical samples. Throat swabs were mixed with known amounts of broth-grown M. pneumoniae cells. An estimated detection limit of less than 40 colony forming units (cfu) was obtained without the need for time-consuming hybridization. The PCR is completed in one day and may be useful for the early detection of M. pneumoniae in clinical samples.
authors.affiliation: Mycoplasma Laboratory, Statens Seruminstitut, Copenhagen, Denmark.
authors.forename: J S
authors.initials: JS
authors.lastname: Jensen
authors.forename: J
authors.initials: J
authors.lastname: Søndergård-Andersen
authors.forename: S A
authors.initials: SA
authors.lastname: Uldum
authors.forename: K
authors.initials: K
authors.lastname: Lind
mesh_terms: D004269:DNA, Bacterial / Q000032:analysis*; D005784:Gene Amplification; D009177:Mycoplasma pneumoniae / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction
chemical_list: D004269:DNA, Bacterial