Detection of intracytoplasmic immunoglobulin by flow cytometry in B-cell malignancies.
The aim of this study was to compare the results of flow cytometric (FCM) determination of heavy and light chain cytoplasmic immunoglobulin (cIg) with those obtained by the peroxidase-antiperoxidase (PAP) method. Fifty-one patients, including five non-T-acute lymphoblastic leukemias, 16 B-chronic lymphocytic leukemias (CLL), 13 non-Hodgkin's lymphomas, seven hairy cell leukemias, four multiple myeloma/plasma cell leukemias, and six T-cell leukemia/lymphomas, as well as 12 normal controls, were studied. Saponin-permeabilized cell suspensions were indirectly stained with monoclonal antibodies and analyzed by flow cytometry. Acetone-fixed cytocentrifuge smears were stained for cIg by the PAP method. The results obtained indicate that: (a) detection of cIg by FCM is a feasible and useful technique to confirm the B-cell lineage of leukemias and lymphomas, particularly those characterized by low-density surface immunoglobulin, such as CLL; and (b) cIg detection by FCM and PAP staining are complementary methods to recognize with certainty the monoclonality of B-cell malignancies.
Bardales
R H
RH
Lymphocyte Surface Markers Laboratory, Memorial Sloan-Kettering Cancer Center, New York, New York 10021.
Al-Katib
A M
AM
Carrato
A
A
Koziner
B
B
D001402:B-Lymphocytes / Q000276:immunology*; D003593:Cytoplasm / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D007124:Immunoenzyme Techniques; D007143:Immunoglobulin Heavy Chains / Q000032:analysis; D007147:Immunoglobulin Light Chains / Q000032:analysis; D007136:Immunoglobulins / Q000032:analysis*; D007938:Leukemia / Q000276:immunology*; D007943:Leukemia, Hairy Cell / Q000276:immunology; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000276:immunology; D007952:Leukemia, Plasma Cell / Q000276:immunology; D015458:Leukemia, T-Cell / Q000276:immunology; D008223:Lymphoma / Q000276:immunology*; D009101:Multiple Myeloma / Q000276:immunology*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology
D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007136:Immunoglobulins