The inhibitory effects by combined doses of DDS and several immunostimulants on the growth of leprosy bacilli inoculated into footpads of hybrid nude mice, Jcl:AF-nu.
The inhibitory effects by the combined doses of bacteriostatic DDS and several immunostimulants on the growth of leprosy bacilli (LB) inoculated into the footpads of nude mice were examined, using a strain of hybrid nude mice named Jcl:AF-nu, which is resistible to infections more than strain BALB/c-nu. The results found were: 1. LB could proliferate up to 10(9) level in the footpads of this strain of hybrid nude mice. 2. The combined dose of muramyl dipeptide (MDP) or a water-soluble lipoidal amine, CP-46665 and DDS mixed with chow in the content ratio of 0.005% completely inhibited the growth of LB. Whereas, the growth inhibition by DDS alone was only partial. 3. A derivative of MDP, muroctasin could partially inhibit the growth of LB without combined dose of DDS. 4. The combined dose of an antitumor beta-1, 3-glucan named ATSO could not enhance the partial growth inhibition due to the dose of DDS through 0.005%-DDS chow. Based on these results, the possibility of Jcl:AF-nu mice as a favorable animal model for examining the synergic inhibitory effect on the growth of LB due to the combined dose of an antileprous chemotherapeutic and an immunostimulant was discussed.
Gidoh
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Tsutsumi
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D000276:Adjuvants, Immunologic / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D003622:Dapsone / Q000494:pharmacology* / Q000627:therapeutic use; D003864:Depression, Chemical; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D005528:Foot / Q000382:microbiology; D007918:Leprosy / Q000188:drug therapy*; D051379:Mice; D008819:Mice, Nude; D009166:Mycobacterium leprae / Q000187:drug effects*
D000276:Adjuvants, Immunologic; D003622:Dapsone