Mass spectrometric identification of peptides present in immunized and parasitised hemolymph from honeybees without purification.
We propose that a substance, identified using mass spectrometry, present in the hemolymph of the honeybee (Apis mellifera) as a result of stimulating the insect immune system corresponds with Apidaecin I, an antibacterial peptide recently described. The plasma desorption mass spectra indicate that several other higher molecule mass substances are synthesised as a result of bacterial or parasite infection.
Craig
A G
AG
Department of Immunology, University of Uppsala, Sweden.
Trenczek
T
T
Fries
I
I
Bennich
H
H
D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification*; D023181:Antimicrobial Cationic Peptides; D016782:Apicomplexa / Q000276:immunology / Q000472:pathogenicity; D001516:Bees / Q000276:immunology* / Q000382:microbiology / Q000469:parasitology; D004754:Enterobacter / Q000276:immunology; D006458:Hemolymph / Q000276:immunology*; D013058:Mass Spectrometry; D010455:Peptides / Q000276:immunology / Q000302:isolation & purification*
D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D010455:Peptides; C061361:apidaecin