The REV1 gene of Saccharomyces cerevisiae: isolation, sequence, and functional analysis.
The REV1 gene of Saccharomyces cerevisiae is required for normal induction of mutations by physical and chemical agents. We have determined the sequence of a 3,485-base-pair segment of DNA that complements the rev1-1 mutant. Gene disruption was used to confirm that this DNA contained the REV1 gene. The sequenced segment contains a single long open reading frame, which can encode a polypeptide of 985 amino acid residues. The REV1 transcript is 3.1 kilobase pairs in length. Frameshift mutations introduced into the open reading frame yielded a Rev-phenotype. A base substitution, encoding Gly-193 to Arg-193, was found in this open reading frame in rev1-1. Deletion mutants, lacking segments of the 5' region of REV1, had intermediate mutability relative to REV1 and rev1-1; a complete deletion exhibited lower mutability than rev1-1. REV1 is not an essential gene. An in-frame fusion of the 5' end of the REV1 open reading frame to the lacZ gene produced beta-galactosidase activity constitutively. The predicted REV1 protein is hydrophilic, with a predicted pI of 9.82. No homologies to RAD1, RAD2, RAD3, RAD7, or RAD10 proteins were noted. A 152-residue internal segment displayed 25% identity with UMUC protein.
Larimer
F W
FW
Biology Division, Oak Ridge National Laboratory, Tennessee 37831.
Perry
J R
JR
Hardigree
A A
AA
D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology; D000595:Amino Acid Sequence; D001483:Base Sequence; D003360:Cosmids; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D005800:Genes, Fungal; D006358:Hot Temperature; D008969:Molecular Sequence Data; D009154:Mutation; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D014158:Transcription, Genetic; D014466:Ultraviolet Rays; D001616:beta-Galactosidase / Q000235:genetics
D004271:DNA, Fungal; D015112:4-Nitroquinoline-1-oxide; D001616:beta-Galactosidase