Effect of intranasal growth hormone-releasing hormone and corticotropin-releasing hormone administration on growth hormone and cortisol release: improved bioavailability by means of sodium-glycocholate.
Several peptide hormones are effective when administered intranasally (in); these include oxytocin, vasopressin, insulin, glucagon, and calcitonin. With regard to GHRH and CRH, previous studies demonstrated that their bioavailability following in administration was very low. In this study we evaluated the serum GH response to 50 micrograms GHRH iv and to 700 micrograms GHRH in, the latter given alone and with 5 and 15 mg sodium-glycocholate (SGC), a surfactant, in six normal men. The bioavailability of in GHRH, calculated as net GH secretory area, was very low, and increased to 7% that of iv GHRH when SGC was used. In the same men, 50 micrograms CRH was administered both iv and in, alone and with 5 and 15 mg SGC. The bioavailability of in CRH, calculated as net cortisol secretory area, was very low and increased to 100% that of iv CRH when 15 mg SGC was used. These data indicate that the efficacy of GHRH and CRH administered in is significantly augmented by SGC.
Pontiroli
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AE
Istituto Scientifico San Raffaele, Cattedra di Clinica Medica, Universita degli Studi di Milano, Italy.
Perfetti
M G
MG
Fattor
B
B
Pozza
G
G
D000281:Administration, Intranasal; D000328:Adult; D000336:Aerosols; D000704:Analysis of Variance; D001682:Biological Availability; D003346:Corticotropin-Releasing Hormone / Q000008:administration & dosage* / Q000493:pharmacokinetics; D006000:Glycocholic Acid / Q000009:adverse effects / Q000494:pharmacology*; D013006:Growth Hormone / Q000097:blood*; D013007:Growth Hormone-Releasing Hormone / Q000008:administration & dosage* / Q000493:pharmacokinetics; D006801:Humans; D006854:Hydrocortisone / Q000097:blood*; D007275:Injections, Intravenous; D008297:Male
D000336:Aerosols; D013006:Growth Hormone; D003346:Corticotropin-Releasing Hormone; D013007:Growth Hormone-Releasing Hormone; D006000:Glycocholic Acid; D006854:Hydrocortisone