title: Recombinant interferon-gamma induces HLA-DR expression on human corneal epithelial and endothelial cells in vitro: a preliminary report.
abstract: The effect of interferon-gamma on the expression in situ of major histocompatibility complex (MHC) products in human corneas was studied in vitro. Incubation for four days with 5 or 50 mg/l of Escherichia coli-derived recombinant human interferon-gamma resulted in the appearance de novo of MHC class II or HLA-DR antigens on variable numbers of corneal epithelial cells as well as on corneal endothelium, whereas it had no effect on the expression of MHC class I or HLA-ABC antigens. These results may help to explain the mechanism underlying the expression of HLA-DR antigens on corneal and limbal epithelium in various inflammatory eye diseases.
authors.affiliation: Department of Ophthalmology, Mansoura University Hospital, Egypt.
authors.forename: A M
authors.initials: AM
authors.lastname: el-Asrar
authors.forename: J J
authors.initials: JJ
authors.lastname: van den Oord
authors.forename: A
authors.initials: A
authors.lastname: Billiau
authors.forename: V
authors.initials: V
authors.lastname: Desmet
authors.forename: M h
authors.initials: Mh
authors.lastname: Emarah
authors.forename: L
authors.initials: L
authors.lastname: Missotten
mesh_terms: D002478:Cells, Cultured; D003315:Cornea / Q000187:drug effects* / Q000276:immunology; D004728:Endothelium, Corneal / Q000187:drug effects / Q000276:immunology; D004848:Epithelium / Q000187:drug effects / Q000276:immunology; D006684:HLA-DR Antigens / Q000032:analysis*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D011994:Recombinant Proteins
chemical_list: D006684:HLA-DR Antigens; D011994:Recombinant Proteins; D007371:Interferon-gamma