Lack of effect of gold salts on acetylcholine or histamine contraction of canine tracheal and bronchial smooth muscle.
In vitro gold salt incubation with proximal and distal canine airway smooth muscle was compared to untreated canine airway muscle with respect to histamine and acetylcholine-induced contraction. Gold chloride and gold sodium thiomalate had no effect on either proximal or distal canine airway smooth muscle agonist-induced contraction. Gold chloride in concentrations greater than 10(-5) M had a direct contractile effect. These results differ from previous studies which showed an inhibition of the histamine response of guinea-pig tracheal smooth muscle following in vitro preincubation with gold salts. This difference suggests that the mechanism(s) of the beneficial effects of long-term gold salt therapy on human asthma cannot be reliably studied with short-term in vitro animal preparations without first assessing species differences with respect to the effects of therapeutic agents. Human airway smooth muscle preparations would be the best means of addressing this question.
Ledford
D K
DK
Department of Internal Medicine, College of Medicine, University of South Florida, Tampa 33612.
Sakamoto
Y
Y
O'Connor
C
C
Krzanowski
K
K
Lockey
R F
RF
Polson
J B
JB
Krzanowski
J
J
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D001980:Bronchi / Q000187:drug effects; D004285:Dogs; D006046:Gold / Q000494:pharmacology*; D017612:Gold Compounds; D006052:Gold Sodium Thiomalate / Q000494:pharmacology; D006632:Histamine / Q000494:pharmacology*; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects*; D014132:Trachea / Q000187:drug effects
D017612:Gold Compounds; D007202:Indicators and Reagents; C038016:gold chloride; D006052:Gold Sodium Thiomalate; D006046:Gold; D006632:Histamine; D000109:Acetylcholine