[Correlation of the excretion of mycoplasma and kinetics of antibodies detected by complement fixation, passive hemagglutination and rapid seroagglutination in Mycoplasma mycoides subsp. mycoides SC experimental infection in cattle].
During an experimental reproduction of CBPP, 5 inoculated cows and 5 contacts cows were bled twice a week and antibodies research was performed using complement fixation test (CFT), passive haemagglutination test (PHAT) and slide agglutination test (SAT). In the same period, trachobronchial washings were performed weekly to detect and to count Mycoplasma mycoides subsp. mycoides SC. For four months these tests were used to compare antibodies kinetics with kinetics of mycoplasmas excretion. With titer over 40 as threshold of positivity, PHAT detects antibodies earlier than CFT and SAT at the beginning of infection, but fails to detect chronic carriers. In contacts cows no failure has been observed with CFT and SAT except during prodromic phase of infection. These results are valid for natural infections. However, for inoculated animals, most serious failures to detect infected cows occur.
Poumarat
F
F
Laboratoire de Pathologie Bovine, Centre National d'Etudes Vétérinaires et Alimentaires, Lyon, France.
Perrin
M
M
Belli
P
P
Martel
J L
JL
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology*; D003168:Complement Fixation Tests; D006386:Hemagglutination Tests; D009176:Mycoplasma mycoides / Q000276:immunology; D011002:Pleuropneumonia, Contagious / Q000276:immunology*; D013997:Time Factors
D000907:Antibodies, Bacterial
Corrélation entre l'excrétion des mycoplasmes et les cinétiques des anticorps mis en évidence par fixation du complément, hémagglutination passive et séroagglutination rapide, au cours d'une infection expérimentale de bovins par Mycoplasma mycoides subsp. mycoides SC.