title: Human IgG antibodies immunoreacting with specific sulfolipids from Mycobacterium tuberculosis.
abstract: IgG and IgM antibodies immunoreacting with sulfolipids from Mycobacterium tuberculosis were detected in sera from tuberculosis patients. The method used was an enzyme linked immunoassay (ELISA) and the antigens were a 2, 3, 6, 6' tetraacyl trehalose-2'-sulfate (sulfolipid I, SL I) and a 2, 3 diacyl trehalose-2'-sulfate (sulfolipid IV, SL IV). The SL IV antigen was satisfactory for IgG assays. The sensitivity and the specificity of the test were, respectively, 59 and 100%; the predictive values of a positive and negative test were, respectively, 100 and 76%.
authors.affiliation: Unité de la Tuberculose et des Mycobactéries, Institut Pasteur, Paris, France.
authors.forename: P
authors.initials: P
authors.lastname: Cruaud
authors.forename: C
authors.initials: C
authors.lastname: Berlie
authors.forename: J
authors.initials: J
authors.lastname: Torgal Garcia
authors.forename: F
authors.initials: F
authors.lastname: Papa
authors.forename: H L
authors.initials: HL
authors.lastname: David
mesh_terms: D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology; D008055:Lipids / Q000276:immunology*; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D011237:Predictive Value of Tests; D014397:Tuberculosis, Pulmonary / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008055:Lipids; C015518:sulfolipids