Determination of sulfadimethoxine, sulfamethoxazole, trimethoprim and their main metabolites in porcine plasma by column switching HPLC.
A HPLC method for the determination of sulfadimethoxine, sulfamethoxazole, trimethoprim and their main metabolites in porcine plasma is reported. The metabolites under investigation were the N4-acetyl sulfonamides and 3'- and 4'-demethyl trimethoprim. In order to obtain a sensitivity of 25-50 ng ml-1, the application of column switching HPLC was investigated. An on-line preconcentration of the drugs and metabolites was preceded by an off-line sample pre-treatment. Parent compounds and metabolites were separated by reversed-phase HPLC followed by UV-detection. The mean recoveries for 4'-demethyl trimethoprim were greater than 80% while the mean recoveries for the other compounds were greater than 90%. Application of the method for analysis of plasma samples obtained from pharmacokinetic studies is described.
Mengelers
M J
MJ
State Institute for Quality Control of Agricultural Products, Wageningen, The Netherlands.
Oorsprong
M B
MB
Kuiper
H A
HA
Aerts
M M
MM
van Gogh
E R
ER
van Miert
A S
AS
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D013056:Spectrophotometry, Ultraviolet; D013412:Sulfadimethoxine / Q000097:blood* / Q000493:pharmacokinetics; D013420:Sulfamethoxazole / Q000097:blood* / Q000493:pharmacokinetics; D013552:Swine; D014295:Trimethoprim / Q000097:blood* / Q000493:pharmacokinetics
D007202:Indicators and Reagents; D013412:Sulfadimethoxine; D014295:Trimethoprim; D013420:Sulfamethoxazole