[The action of amino acid di[2-haloethyl]hydrazides on DNA synthesis in normal and tumor cells and on the cell cycle kinetics in mice with melanoma B-16].
A comparative study was carried out with two alkylating agents IMB-MM and IMB-97 which are di-(2-halogenoethyl) hydrazides of amino acid derivatives. They have been found to exert a high activity towards wide spectrum of experimental tumours. Both agents caused inhibition of incorporation of 3H-thymidine into DNA of melanoma B16, marrow, intestinal mucosa, spleen and liver cells of mice with tumours. A maximal inhibition of DNA synthesis in all tissues was observed 24 h after the single doses of drugs. However 96 h later this effect was removed excluding the tumour cells. The cytofluorimetric study have shown that IMB-MM, like sarcolysine, caused an accumulation of tumour cells in G2/M phase of cell cycle, while IMB-97 increased accumulation of S-phase cells. The difference in phase sensitivity of tumour cells towards IMB-MM and IMB-97 is due to the differences in aminoacid carriers of di-(2-halogenethyl) hydrazide groups.
Gorneva
G A
GA
Bergol'ts
V V
VV
Mladenova
I P
IP
Smirnova
Z S
ZS
Nikolaeva
T G
TG
D000477:Alkylating Agents / Q000494:pharmacology* / Q000627:therapeutic use; D000596:Amino Acids / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002453:Cell Cycle / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects*; D004273:DNA, Neoplasm / Q000096:biosynthesis / Q000187:drug effects*; D004353:Drug Evaluation, Preclinical; D006834:Hydrazines / Q000494:pharmacology* / Q000627:therapeutic use; D008546:Melanoma, Experimental / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D008558:Melphalan / Q000494:pharmacology / Q000627:therapeutic use; D051379:Mice; D009368:Neoplasm Transplantation; D013997:Time Factors
D000477:Alkylating Agents; D000596:Amino Acids; D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D006834:Hydrazines; D004247:DNA; D008558:Melphalan
Deĭstvie di-[2-galoétil]gidrazidov aminokislot na sintez DNK v normal'nykh i opukholevykh kletkakh i kinetiku kletochnogo tsikla u mysheĭ s melanomoĭ B-16.