The complete amino-acid sequence of the alpha and beta subunits of B-phycoerythrin from the rhodophytan alga Porphyridium cruentum.
Determination of the complete amino-acid sequence of the subunits of B-phycoerythrin from Porphyridium cruentum has shown that the alpha subunit contains 164 amino-acid residues and the beta subunit contains 177 residues. When the sequences of B- and C-phycoerythrins are aligned with those of other phycobiliproteins, it is obvious that B-phycoerythrin lacks a deletion at beta-21-22 present in C-phycoerythrin. However, relative to C-phycoerythrin from Fremyella diplosiphon (Calothrix) (Sidler, W., Kumpf, B., Rüdiger, W. and Zuber, H. (1986) Biol. Chem. Hoppe-Seyler 367, 627-642), B-phycoerythrin has deletions at beta-141k-o, beta-142, beta-143, beta-147 and beta-148. The four singly-linked phycoerythrobilins at positions alpha-84, alpha-143a, beta-84 and beta-155, and the doubly-linked phycoerythrobilin at position beta-50/61 are at sites homologous to the attachment sites in C-phycoerythrin. The aspartyl residues (alpha-87, beta-87, and beta-39), that interact with the bilins at alpha-84, beta-84, and beta-155 in C-phycocyanin, are found in the homologous positions in B-phycoerythrin. B-Phycoerythrin, in common with other phycobiliproteins, contains a N gamma-methylasparagine residue at position beta-72.
Sidler
W
W
Institut für Molekularbiologie und Biophysik, Eidgenössiche Technische Hochschule, Zürich.
Kumpf
B
B
Suter
F
F
Klotz
A V
AV
Glazer
A N
AN
Zuber
H
H
D000595:Amino Acid Sequence; D002268:Carboxypeptidases; D043422:Carboxypeptidases A; D002848:Chromatography, DEAE-Cellulose; D000458:Cyanobacteria / Q000378:metabolism; D003488:Cyanogen Bromide; D006868:Hydrolysis; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis; D010447:Peptide Hydrolases; D010799:Phycoerythrin / Q000032:analysis*; D010860:Pigments, Biological / Q000032:analysis*; D000461:Rhodophyta / Q000378:metabolism*; D012862:Skatole / Q000031:analogs & derivatives; D013210:Staphylococcus / Q000201:enzymology
D010446:Peptide Fragments; D010860:Pigments, Biological; D010799:Phycoerythrin; C005904:BNPS-skatole; D012862:Skatole; D002268:Carboxypeptidases; D010447:Peptide Hydrolases; D043422:Carboxypeptidases A; D003488:Cyanogen Bromide