Suppressing effect of tannic acid on the frequencies of mutagen-induced sister-chromatid exchanges in mammalian cells.
The effects of tannic acid (m-galloyl gallic acid) and 7 of its analogues on the frequencies of sister-chromatid exchanges (SCEs) were investigated in cultured Chinese hamster cells. SCEs induced by UV-light or mitomycin C (MMC) were suppressed by post-treatment with tannic acid and 5 of its analogues. These effects were independent of the extension of the cell cycle. The compounds which showed an SCE-suppressing effect have a common structure of 3 neighboring hydroxy or methoxy groups substituted on the phenyl group in benzoic acid or ester. These decreasing effects of tannic acid were observed in the G1 phase but not in the S or G2 phase of the cell cycle and a greater decline of the frequencies of UV-induced SCEs during liquid holding was seen in the presence of tannic acid. However, cells irradiated with X-rays were not influenced by tannic acid. In cells from a xeroderma pigmentosum (XP) patient, a Fanconi's anemia (FA) patient, and a normal human embryo, MMC-induced SCEs were also decreased by post-treatment with tannic acid. Tannic acid reduced the SCE frequencies in UV-irradiated FA and normal human cells but not in UV-irradiated XP cells. Our results suggest that tannic acid modifies DNA-excision repair and that the decrease in the amount of unrepaired DNA damage might cause the reduction of induced SCEs.
Sasaki YuF
Institute of Environmental Toxicology, Tokyo, Japan.
Imanishi
H
H
Ohta
T
T
Watanabe
M
M
Matsumoto
K
K
Shirasu
Y
Y
D000818:Animals; D002460:Cell Line; D055598:Chemical Phenomena; D002621:Chemistry; D006224:Cricetinae; D003412:Cricetulus; D004260:DNA Repair / Q000187:drug effects*; D047348:Hydrolyzable Tannins / Q000031:analogs & derivatives / Q000494:pharmacology*; D016685:Mitomycin; D008937:Mitomycins / Q000633:toxicity; D009153:Mutagens; D012854:Sister Chromatid Exchange / Q000187:drug effects* / Q000528:radiation effects; D013634:Tannins / Q000494:pharmacology*; D014466:Ultraviolet Rays
D047348:Hydrolyzable Tannins; D008937:Mitomycins; D009153:Mutagens; D013634:Tannins; D016685:Mitomycin