Potentiation of invasive capacity of rat ascites hepatoma cells by transforming growth factor-beta.
The in vitro invasive capacity of poorly invasive cells (W1), which were cloned from rat ascites hepatoma cells (AH 130), was potentiated dose- and time-dependently by pretreating the cells with transforming growth factor-beta (TGF-beta). This potentiation of invasive capacity was completely abolished by anti-TGF-beta antibody. When the treated cells were ip inoculated into rats, the cells extensively invaded the peritoneum, and formed penetrating tumor nodules. The effect of TGF-beta was reversed by subculturing the treated cells without TGF-beta. The potentiation of invasive capacity by TGF-beta might participate in platelet-associated enhancement of tumor cell metastasis.
Mukai
M
M
Department of Tumor Biochemistry, Center for Adult Diseases, Osaka.
Shinkai
K
K
Komatsu
K
K
Akedo
H
H
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002999:Clone Cells; D004305:Dose-Response Relationship, Drug; D008114:Liver Neoplasms, Experimental / Q000473:pathology*; D008297:Male; D009361:Neoplasm Invasiveness; D010534:Peritoneal Neoplasms / Q000473:pathology / Q000556:secondary*; D051381:Rats; D015291:Transforming Growth Factors / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D015291:Transforming Growth Factors