Evaluation of ethylenediaminetetraacetic acid, tetrasodium salt dihydrate (EDTA)-Tween 20 treatment versus protease digestion of formalin-fixed tissue sections for detection of bovine respiratory syncytial virus antigen in infected ovine lung.
The efficacy of protease and ethylenediaminetetraacetic acid, tetrasodium salt dihydrate (EDTA)-Tween 20 in unmasking bovine respiratory syncytial virus (BRSV) antigens in formalin-fixed lung tissue was compared using avidin-biotin immunoperoxidase procedure. Tissues were taken from experimentally infected lambs. BRSV antigen stained in both techniques. Treatment with EDTA-Tween 20 resulted in more intense staining of BRSV infected cells, more uniform cytoplasmic staining, less non-specific background, and superior cellular detail in comparison to protease digestion.
Meehan
J T
JT
National Animal Disease Center, Ames, IA.
Cutlip
R C
RC
Lehmkuhl
H D
HD
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D004492:Edetic Acid; D010450:Endopeptidases; D008168:Lung / Q000382:microbiology*; D011136:Polysorbates; D012136:Respiratory Syncytial Viruses / Q000276:immunology* / Q000302:isolation & purification; D012141:Respiratory Tract Infections / Q000175:diagnosis / Q000662:veterinary; D010253:Respirovirus Infections / Q000175:diagnosis / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis*
D000956:Antigens, Viral; D011136:Polysorbates; D004492:Edetic Acid; D010450:Endopeptidases