title: In vitro responsiveness of human testicular DNA synthesis to gonadotropins.
abstract: To evaluate the responsiveness of spermatogenesis in the normal human testis to human menopausal gonadotropin (hMG) and human chorionic gonadotropin (hCG), the DNA synthesis of spermatogonia was investigated using 3H-thymidine uptake by cultured tissue fragments of human testis with or without treatment with hCG and/or hMG. Two groups treated with different concentrations of hCG showed no difference in 3H-thymidine incorporation into testicular tissue compared with untreated control. In two other groups treatment with combined hMG/hCG in different concentrations induced significant changes in 3H-thymidine incorporation compared with control (p less than 0.01, p less than 0.01). Combined hMG/hCG treatment was effective in inducing spermatogenesis by normal human testicular tissue.
authors.affiliation: Department of Urology and Pathology, Osaka University Medical School, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Okuyama
authors.forename: N
authors.initials: N
authors.lastname: Nonomura
authors.forename: E
authors.initials: E
authors.lastname: Koh
authors.forename: N
authors.initials: N
authors.lastname: Kondoh
authors.forename: M
authors.initials: M
authors.lastname: Nakamura
authors.forename: M
authors.initials: M
authors.lastname: Namiki
authors.forename: H
authors.initials: H
authors.lastname: Kiyohara
authors.forename: Y
authors.initials: Y
authors.lastname: Nishimune
authors.forename: K
authors.initials: K
authors.lastname: Matsumoto
authors.forename: T
authors.initials: T
authors.lastname: Sonoda
mesh_terms: D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D046508:Culture Techniques; D004247:DNA / Q000096:biosynthesis*; D006801:Humans; D008297:Male; D008596:Menotropins / Q000494:pharmacology*; D013091:Spermatogenesis / Q000187:drug effects; D013737:Testis / Q000187:drug effects / Q000378:metabolism*
chemical_list: D006063:Chorionic Gonadotropin; D008596:Menotropins; D004247:DNA