Protein fingerprinting: a novel virus identification system.
Viral proteins separated by one-dimensional SDS-PAGE produce protein binding patterns (fingerprints) which are unique for different viruses. We have applied this concept successfully for the development of a practical and objective virus identification system which is applicable to most viruses. The method is simple, specific, and, unlike the currently available methods, free from all virus-specific reagents. Interference by host protein bands in SDS-PAGE preparations of virus-infected cell lysates was eliminated consistently by treating virus infected cell cultures with optimum concentration of NaCl for selective inhibition of host protein synthesis. The method utilizes the comparison of protein fingerprints of 'unknown' viruses with protein fingerprints of reference viruses stored in a computer data base, using pattern recognition software. All 113 'unknown' virus strains were correctly identified to the genus level by the protein fingerprint method, when compared with the conventional virus identification methods.
Walpita
P
P
Department of Pediatrics, University of California, San Diego, La Jolla 92093.
Connor
J D
JD
Pfeifer
D
D
D000818:Animals; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007256:Information Systems; D010449:Peptide Mapping / Q000379:methods*; D012984:Software; D014764:Viral Proteins / Q000032:analysis*; D014779:Virus Replication; D014780:Viruses / Q000032:analysis*
D014764:Viral Proteins