Suppressive effect of interferon-gamma on the BCL1 cell-dependent interleukin 5 bioassay.
Proliferation of BCL1 cells, generally used for the measurement of murine interleukin (IL)5, is shown to be strongly suppressed by interferon (IFN)-gamma. This effect can be abrogated by the addition of anti-IFN-gamma antibodies. Thus, the IL5 activity of culture supernatants derived from T cells simultaneously producing IFN-gamma and IL5, can only be seen with BCL1 cells in the presence of anti-IFN-gamma antibodies. If anti-IFN-gamma antibodies are omitted, T cells which produce both, IFN-gamma and IL5, may mistakenly be grouped into the TH1 subtype producing only IFN-gamma, but no IL5.
Lohoff
M
M
Institut für klinische Mikrobiologie, Universität Erlangen-Nürnberg, FRG.
Sommer
F
F
Röllinghoff
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D001402:B-Lymphocytes / Q000502:physiology; D001681:Biological Assay / Q000592:standards; D007371:Interferon-gamma / Q000494:pharmacology*; D015847:Interleukin-4; D015848:Interleukin-5; D007378:Interleukins / Q000032:analysis* / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D015848:Interleukin-5; D007378:Interleukins; D011994:Recombinant Proteins; D015847:Interleukin-4; D007371:Interferon-gamma