title: Determination of aminoglycosides in pharmaceutical formulations--II. High-performance liquid chromatography.
abstract: A post-column derivatization procedure using OPA and fluorescence detection has been used for the determination of seven aminoglycosides (dibekacin, framycetin, kanamycin, netilmicin, sisomicin, tobramycin and gentamicin) in commercial pharmaceutical formulations. The linearity, precision and detection limits were satisfactory. Recoveries from eye drops, ointments, injections and capsules were comparable (P = 0.05) to those obtained with TLC or microbiological assays. A ruggedness test showed that the method was not sensitive to minor variations in the mobile phase composition, post-column derivatization system or detection wavelength.
authors.affiliation: Faculté de Pharmacie, Montpellier, France.
authors.forename: H
authors.initials: H
authors.lastname: Fabre
authors.forename: M
authors.initials: M
authors.lastname: Sekkat
authors.forename: M D
authors.initials: MD
authors.lastname: Blanchin
authors.forename: B
authors.initials: B
authors.lastname: Mandrou
mesh_terms: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000032:analysis*; D001681:Biological Assay; D002214:Capsules; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D009824:Ointments; D009883:Ophthalmic Solutions; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D009764:o-Phthalaldehyde
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D002214:Capsules; D009824:Ointments; D009883:Ophthalmic Solutions; D012996:Solutions; D009764:o-Phthalaldehyde