A simple and fast method for iron determination with ferrozine after proteolytic disruption of iron-transfer in complex.
A direct method for iron determination with ferrozine in blood serum is described. Iron is liberated and proteins degraded by pepsin in hydrochloric acid medium. At appropriate pH the iron-ferrozine complex forms and stabilizes in five minutes. Accuracy of the method is 99.3-101.4%, precision within run and day to day 0.73 and 1.1% respectively, and linearity till 72 mumol/L iron.
Markovic
M
M
Straus
B
B
D000328:Adult; D005260:Female; D005297:Ferrozine; D006801:Humans; D007501:Iron / Q000097:blood*; D008297:Male; D008875:Middle Aged; D010434:Pepsin A / Q000378:metabolism*; D010783:Photometry; D012016:Reference Values; D014168:Transferrin / Q000378:metabolism*; D014227:Triazines
D014168:Transferrin; D014227:Triazines; D005297:Ferrozine; D007501:Iron; D010434:Pepsin A