[Immunisation of spontaneously hypertensive rats against angiotensin I].
Immunization against angiotensin I has been considered in comparison with the immunization against renin, in the spontaneously hypertensive rat (SHR). Among 6 different methods of immunization, two (AI-gluta-LPH and AI-Carbo-LPH coupling) permitted to obtain high levels of antibodies against angiotensin I (higher than 1/10,000), after four injections of 50 micrograms AI at three weeks interval. The titration of the antibodies was realized in radio-immuno-assay (RIA), with the determination of the cross-reactivity with AII by the same method. Characterization of the isotypes and the affinity calculation were realized with the ELISA method. The average level of antibodies is about 1/10,000 to 1/100,000, and the cross reactivity of the antibodies for AII is about 0.1 p. 100 in RIA. In ELISA, the study of the different isotypes shows a good maturation of the immune system with a sharp elevation of the IgG1 and IgG2 alpha isotypes, after 2 or 3 injections. The affinity of the antibodies purified by affinity chromatography is about 10.3 10(-9) M. The weekly measure of the arterial pressure during 6 months does not reveal at any moment a fall of pressure during the immunizations. The average pressure of the immunized group (209.4 +/- 23.8 mmHg, n = 40) is non significantly different from the average pressure of the mock group (208.5 +/- 22.6 mmHg, n = 10).
Reade
R
R
INSERM U 36, Paris.
Michel
J B
JB
Carelli
C
C
Huang
H
H
Baussant
T
T
Corvol
P
P
D000803:Angiotensin I / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D006973:Hypertension / Q000628:therapy*; D007114:Immunization; D008297:Male; D051381:Rats; D011918:Rats, Inbred SHR; D012084:Renin-Angiotensin System
D000906:Antibodies; D000803:Angiotensin I
Immunisation du rat spontanément hypertendu contre l'angiotensine I.