title: Human cardiolipin as the antigen in an ELISA to detect anticardiolipin antibodies.
abstract: Human cardiolipin was purified from cadaver heart tissue and used as the antigen in an enzyme-linked immunoassay (ELISA) for anticardiolipin antibodies. The correlation between assays using human and bovine cardiolipin was r = 0.90. Of the lupus anticoagulant positive patients 88% had antibodies to the human antigen and 75% had antibodies to the bovine antigen. Six of 45 samples contained antibodies which only reacted with the human antigen. All six samples remained positive when retested with a different human cardiolipin preparation and were negative when tested against a preparation of sheep cardiolipin. These studies suggest that there was increased specificity when human cardiolipin was used as the phospholipid antigen.
authors.affiliation: Haematology Department, Royal Prince Alfred Hospital, Camperdown, NSW, Australia.
authors.forename: R D
authors.initials: RD
authors.lastname: Brown
authors.forename: J
authors.initials: J
authors.lastname: Gibson
authors.forename: J
authors.initials: J
authors.lastname: Wells
authors.forename: R
authors.initials: R
authors.lastname: Jun
authors.forename: K
authors.initials: K
authors.lastname: Rickard
authors.forename: H
authors.initials: H
authors.lastname: Kronenberg
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis*; D001779:Blood Coagulation Factors / Q000032:analysis / Q000276:immunology; D002308:Cardiolipins / Q000276:immunology; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology; D007075:Immunoglobulin M / Q000032:analysis / Q000276:immunology; D016682:Lupus Coagulation Inhibitor; D012756:Sheep
chemical_list: D000906:Antibodies; D001779:Blood Coagulation Factors; D002308:Cardiolipins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D016682:Lupus Coagulation Inhibitor