Determination of serum atenolol using HPLC with fluorescence detection following isolation with activated charcoal.
In order to determine the bio-availability and pharmacokinetics of two oral 100 mg atenolol preparations, a simple analytical method was developed. Atenolol was determined in serum submitted to an extraction procedure consisting of: (a) adsorption of atenolol to activated charcoal at pH 11, (b) washing the charcoal with water to remove co-extracts and (c) elution of atenolol from the charcoal with organic solvent. The extracts were then analysed by high-performance liquid chromatography (HPLC) with fluorescence detection. The pharmacokinetic parameters obtained from eight healthy humans involved in a clinical bioavailability trial are also presented.
Alebić-Kolbah
T
T
Pliva Research Institute, Zagreb, Yugoslavia.
Plavsić
F
F
Wolf-Coporda
A
A
D000328:Adult; D001262:Atenolol / Q000097:blood*; D001682:Biological Availability; D002606:Charcoal; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D008297:Male; D013050:Spectrometry, Fluorescence
D002606:Charcoal; D001262:Atenolol