title: Ultrastructural changes induced by experimental subarachnoid haemorrhage and 6-hydroxydopamine in cat cerebral arteries.
abstract: Transmission electron microscopy of the middle cerebral artery from cats exposed to subarachnoid injection of blood 3 and 7 days before, showed several damage of the vascular ultrastructure. The intima was thickened with swelling and vacuolization of endothelial cells, with a plump appearance and disruption of tight junctions. The cellular surface was corrugated and the subendothelial space contained proliferating and vacuolated smooth muscle cells capped by elastin and collagen fibres. The internal elastic lamina was also corrugated and disrupted. The adventitial changes were axonic cytoplasmic and mitochondrial swelling, virtual absence of synaptic vesicles, and disruption of the outer axonal membrane, suggesting denervation of cerebral vessels. With administration of 6-hydroxy-dopamine (6-OHDA), similar ultrastructural changes were observed in the adventitia. These observations indicate that denervation induced by subarachnoid bleeding could be involved in the pathophysiology of cerebrovascular spasm. Subarachnoid haemorrhage, but not 6-OHDA, affects also intima and tunica media, suggesting other mechanisms, in addition to denervation, can participate in the vasospasm.
authors.affiliation: Department of Pharmacology and Therapeutics, School of Medicine, Autonomous University, Madrid, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Rivilla
authors.forename: J
authors.initials: J
authors.lastname: Marín
authors.forename: C F
authors.initials: CF
authors.lastname: Sánchez-Ferrer
authors.forename: M
authors.initials: M
authors.lastname: Salaices
authors.forename: P G
authors.initials: PG
authors.lastname: Ramos
mesh_terms: D000818:Animals; D002415:Cats; D002536:Cerebral Arteries / Q000187:drug effects / Q000648:ultrastructure*; D005260:Female; D006892:Hydroxydopamines / Q000633:toxicity*; D008297:Male; D008854:Microscopy, Electron; D009498:Neurotoxins; D016627:Oxidopamine; D013345:Subarachnoid Hemorrhage / Q000473:pathology*
chemical_list: D006892:Hydroxydopamines; D009498:Neurotoxins; D016627:Oxidopamine