Pathways for the synthesis of sorbitol from 13C-labeled hexoses, pentose, and glycerol in renal papillary tissue.
Suspensions of rabbit renal papillary tissue were incubated with D-[6-13C]glucose, D-[1-13C]fructose, D-[1-13C]ribose, and [2-13C]glycerol. The perchloric acid extracts of the above incubations were investigated with 13C NMR spectroscopy. All 13C-labeled substrates give rise to 13C-labeled D-sorbitol. D-[6-13C]Glucose and D-[1-13C]fructose are converted directly into D-sorbitol via the aldose reductase and sorbitol dehydrogenase pathway, respectively, whereas D-[1-13C]ribose and [2-13C]glycerol give rise to labeling of the D-glyceraldehyde pool which on its turn causes a labeling of D-sorbitol. Label exchanges observed from incubations with glycerol and D-ribose indicate that the pentose shunt plays a role in this synthesis of D-sorbitol.
Jans
A W
AW
Max-Planck-Institut fuer Systemphysiologie, Dortmund, Federal Republic of Germany.
Grunewald
R W
RW
Kinne
R K
RK
D000818:Animals; D005947:Glucose / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism*; D066298:In Vitro Techniques; D007679:Kidney Medulla / Q000378:metabolism*; D007064:L-Iditol 2-Dehydrogenase / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D010427:Pentose Phosphate Pathway; D011817:Rabbits; D012266:Ribose / Q000378:metabolism*; D013012:Sorbitol / Q000096:biosynthesis*
D013012:Sorbitol; D012266:Ribose; D007064:L-Iditol 2-Dehydrogenase; D005947:Glucose; D005990:Glycerol