Aphidicolin inhibition of gamma-radiation-induced DNA repair in human lymphocyte subpopulations.
1. DNA repair was measured in 3 Gy gamma-irradiated human peripheral lymphocyte subpopulations by means of nucleoid sedimentation. 2. The influence of aphidicolin (an inhibitor of DNA polymerase) on the repair process was investigated. 3. Repair of 40-44% of the DNA lesions induced by gamma-irradiation was blocked by aphidicolin. 4. Enriched B- and T-lymphocyte fractions were affected by aphidicolin to the same extent.
van Rensburg
E J
EJ
AEC Institute for Life Sciences, University of Pretoria, South Africa.
Louw
W K
WK
Engelbrecht
R I
RI
Izatt
H L
HL
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D016590:Aphidicolin; D001402:B-Lymphocytes / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D004260:DNA Repair / Q000187:drug effects* / Q000528:radiation effects; D004224:Diterpenes / Q000494:pharmacology*; D005260:Female; D005720:Gamma Rays; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects
D000903:Antibiotics, Antineoplastic; D004224:Diterpenes; D016590:Aphidicolin