title: Crystallization and preliminary X-ray diffraction studies of bovine recombinant immune interferon.
abstract: Reproducible conditions have been established for the crystallization of recombinant bovine immune interferon. Two cystalline forms of this protein were obtained. A tetragonal form, space group P422, with unit cell dimensions a = b = 59.0 A and c = 125.7 A and an orthorhombic form, space group P2(1)2(1)2(1), with unit cell dimensions a = 42.80 A, b = 79.90 A and c = 85.64 A were obtained under similar crystallization conditions. The orthorhombic form diffracts to 2.6 A resolution, contains a single interferon dimer in the asymmetric unit of structure and is suitable for X-ray diffraction analysis.
authors.affiliation: Department of Biomolecular Chemistry, Genentech Inc., South San Francisco, CA 94080.
authors.forename: J R
authors.initials: JR
authors.lastname: Rubin
authors.forename: L E
authors.initials: LE
authors.lastname: Burton
mesh_terms: D000818:Animals; D002417:Cattle; D003460:Crystallization; D007371:Interferon-gamma / Q000032:analysis*; D011487:Protein Conformation; D011994:Recombinant Proteins; D014961:X-Ray Diffraction
chemical_list: D011994:Recombinant Proteins; D007371:Interferon-gamma