A strategy for producing single-stranded DNA in the polymerase chain reaction. A direct method for genomic sequencing.
A strategy for synthesizing single-stranded DNA by the polymerase chain reaction method is explored and two protocols are developed. DNA produced with these methods can be sequenced using standard 35S or 32P dideoxy sequencing protocols. The methods allow rapid genetic screening and population genetic analysis of sequence variation.
Kreitman
M
M
Biology Department, Princeton University, New Jersey 08544.
Landweber
L F
LF
D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D004277:DNA, Single-Stranded / Q000138:chemical synthesis*; D004259:DNA-Directed DNA Polymerase; D004331:Drosophila melanogaster / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005784:Gene Amplification; D005821:Genetic Techniques; D009115:Muridae; D019914:Taq Polymerase
D004277:DNA, Single-Stranded; D019914:Taq Polymerase; D004259:DNA-Directed DNA Polymerase