title: Sensitive determination of circulating anodic antigen in Schistosoma mansoni infected individuals by an enzyme-linked immunosorbent assay using monoclonal antibodies.
abstract: From a panel of mouse monoclonal antibodies reactive with a repeating epitope of the schistosome circulating anodic antigen, an IgG1 monoclonal antibody was selected. This monoclonal antibody was applied in a sandwich enzyme-linked immunosorbent assay as capture antibody and as alkaline phosphatase labeled conjugate. This assay allowed a sensitive quantitation of circulating anodic antigen in serum samples of infected individuals, detecting less than 1 ng antigen/ml serum. In Schistosoma mansoni infected individuals from Zaire, the level of antigen in serum correlated with fecal egg output. The lower detection level of the immunoassay corresponded to a level of about 10 eggs/gm feces.
authors.affiliation: Laboratory of Parasitology, State University of Leiden, The Netherlands.
authors.forename: A M
authors.initials: AM
authors.lastname: Deelder
authors.forename: N
authors.initials: N
authors.lastname: De Jonge
authors.forename: O C
authors.initials: OC
authors.lastname: Boerman
authors.forename: Y E
authors.initials: YE
authors.lastname: Fillié
authors.forename: G W
authors.initials: GW
authors.lastname: Hilberath
authors.forename: J P
authors.initials: JP
authors.lastname: Rotmans
authors.forename: M J
authors.initials: MJ
authors.lastname: Gerritse
authors.forename: D W
authors.initials: DW
authors.lastname: Schut
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth / Q000032:analysis*; D015023:Democratic Republic of the Congo; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D010270:Parasite Egg Count / Q000379:methods; D012550:Schistosoma mansoni / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000175:diagnosis; D012680:Sensitivity and Specificity; D012698:Serologic Tests; D014238:Trichloroacetic Acid
chemical_list: D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D014238:Trichloroacetic Acid