Effect of heat on extracted HIV viral infectivity and antibody activity using the filter paper technique of blood sampling.
The diagnostic potential of the standardized filter paper technique at an outpatient clinic was further evaluated for individuals infected with HIV; the inactivation of HIV in dried blood from filter paper was also investigated. Heat treatment at 56 degrees C or 70 degrees C for 1 h did not demonstrably affect antibody activity as measured by enzyme-linked immunosorbent assay and Western blot. Prolonged treatment up to 18 h reduced the activity to some extent. Drying at room temperature of filter papers which had been soaked with HIV was sufficient to inactivate low, but not high, amounts of infectious virus. After exposure of the filter paper discs to 56 degrees C or 70 degrees C for 1 h, HIV could not be isolated from the extracts. The obvious advantage of blood collection on filter paper for serological studies makes it an alternative to venipuncture, especially when the blood might be contagious for HIV.
Evengård
B
B
Department of Parasitology, Karolinska Institute, Roslagstull Hospital, Stockholm, Sweden.
Ehrnst
A
A
von Sydow
M
M
Pehrson
P O
PO
Lundbergh
P
P
Linder
E
E
D015492:AIDS Serodiagnosis / Q000379:methods*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005374:Filtration / Q000295:instrumentation; D006678:HIV / Q000276:immunology / Q000302:isolation & purification / Q000472:pathogenicity*; D015483:HIV Antibodies / Q000032:analysis*; D006358:Hot Temperature; D006801:Humans; D010209:Paper; D013048:Specimen Handling / Q000379:methods*; D014776:Virus Cultivation
D015483:HIV Antibodies