Isolation and characterization of the Drosophila translational elongation factor 2 gene.
We have isolated a cDNA clone that encodes the Drosophila melanogaster elongation factor 2 (EF2), a protein involved in the elongation step of protein synthesis. This identification was based on the high degree of its amino acid sequence identity (greater than 80%) to that of hamster EF2. The gene encoding Drosophila EF2 is found at position 39E-F of the 2L chromosomal arm and maybe identical to the M(2)H locus, which produces a Minute phenotype when mutated. The genomic organization of the locus includes four exons. Conserved sequence segments shared with a variety of GTP binding proteins are found in the amino terminal third of the protein, and segments unique to EF2 and its prokaryotic functional homolog, EF-G, are in the carboxy terminal half; these two regions are segregated in two respective exons.
Grinblat
Y
Y
Biological Laboratories, Harvard University, Cambridge, MA 02138.
Brown
N H
NH
Kafatos
F C
FC
D000367:Age Factors; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D006224:Cricetinae; D003429:Cross Reactions; D004247:DNA / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics*; D015852:Eukaryotic Initiation Factor-2 / Q000235:genetics* / Q000276:immunology; D019204:GTP-Binding Proteins / Q000235:genetics; D005786:Gene Expression Regulation; D005796:Genes; D016023:Integrins / Q000276:immunology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011233:Precipitin Tests; D015183:Restriction Mapping
D015852:Eukaryotic Initiation Factor-2; D016023:Integrins; D004247:DNA; D019204:GTP-Binding Proteins