Comparative evaluation of the diagnostic significance of circulating immune complexes and antibodies to phosphatidylinositomannosides in pulmonary tuberculosis by enzyme-linked immunosorbent assay.
An enzyme-linked immunosorbent assay was developed to detect circulating immune complexes (CIC) and antibodies to phosphatidylinositomannosides (PIM) of mycobacteria for the diagnosis of pulmonary tuberculosis. Identification of PIM in CIC of smear and culture positive (SPCP) cases revealed 88% sensitivity and antibodies to PIM were detected in circulation in 94% cases. The smear negative and culture positive (SNCP) group had 67% positivity with regards to PIM in CIC and 83% for antibodies to PIM in circulation. In the smear negative and culture negative (SNCN) group, 40% samples were found to have PIM in CIC and 20% exhibited antibodies to PIM. The high degree of sensitivity was observed with both antigen detection in CIC and antibodies to PIM in circulation.
Mehta
P K
PK
Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Khuller
G K
GK
D000907:Antibodies, Bacterial / Q000032:analysis* / Q000276:immunology; D000936:Antigen-Antibody Complex / Q000032:analysis*; D000942:Antigens, Bacterial / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D010716:Phosphatidylinositols / Q000276:immunology*; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000276:immunology
D000907:Antibodies, Bacterial; D000936:Antigen-Antibody Complex; D000942:Antigens, Bacterial; D010716:Phosphatidylinositols; C054977:phosphatidylinositol dimannoside