Immunomagnetic T-lymphocyte depletion (ITLD) of rat bone marrow using OX-19 monoclonal antibody.
Graft versus host disease (GVHD) may be abrogated and host survival prolonged by in vitro depletion of T lymphocytes from bone marrow (BM) prior to allotransplantation. Using a mouse anti-rat pan T-lymphocyte monoclonal antibody (OX19) bound to monosized, magnetic, polymer beads, T lymphocytes were removed in vitro from normal bone marrow. The removal of the T lymphocytes was confirmed by flow cytometry. Injection of the T-lymphocyte-depleted bone marrow into fully allogeneic rats prevents the induction of GVHD and prolongs host survival.
Hoffman
A L
AL
Department of Surgery, University of Pittsburgh School of Medicine, PA 15261.
Makowka
L
L
Cramer
D V
DV
Cai
X
X
Pascualone
A
A
Banner
B F
BF
Sheahan
D G
DG
Rettinger
P
P
Starzl
T E
TE
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation / Q000276:immunology*; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D005434:Flow Cytometry; D006086:Graft vs Host Disease / Q000517:prevention & control; D008212:Lymphocyte Depletion; D008280:Magnetics; D051381:Rats; D011917:Rats, Inbred Lew / Q000276:immunology; D011919:Rats, Inbred Strains / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal