[Potato 5-lipoxygenase. Kinetics of linoleic acid oxidation].
The role of main factors influencing the rate of potato 5-lipoxygenase oxidation of linoleic acid was investigated. It was found that nonionic detergent lubrol PX inhibited the potato lipoxygenase. Optimal pH for the linoleic acid oxidation was 6.3 temperature--45 degrees C and substrate concentration--3 x 10(-4) M (if lubrol PX was 0.02%). It was shown that potato 5-lipoxygenase was allosteric enzyme which possessed positive cooperativity for linoleic acid. The Hill coefficient was calculated (n = 1.40 +/- 0.15) with S0.5 = 75 +/- 10 microM.
Butovich
I A
IA
Kukhar'
V P
VP
D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008041:Linoleic Acids / Q000378:metabolism*; D008084:Lipoxygenase / Q000378:metabolism*; D010084:Oxidation-Reduction; D000077423:Polidocanol; D011092:Polyethylene Glycols; D011198:Solanum tuberosum / Q000201:enzymology*
D008041:Linoleic Acids; D000077423:Polidocanol; D011092:Polyethylene Glycols; D008084:Lipoxygenase
5-lipoksigenaza kartofelia. Kinetika okisleniia linolevoĭ kisloty.