title: Induction of testicular DNA synthesis in vitro by hCG and hMG treatments in men with hypogonadotropic hypogonadism.
abstract: To study the effectiveness of hCG and hMG administration on spermatogenesis of patients with hypogonadotropic hypogonadism (HH), the DNA synthesis of spermatogonia was investigated by the method of 3H-thymidine uptake by cultured tissue fragments of human testis with or without treatment with hCG and/or hMG. Although in two groups treated with different concentrations of hCG the value of 3H-thymidine incorporation into testicular tissue showed no significant difference compared with the untreated control group, treatment with both hCG and hMG induced significant changes in the value of 3H-thymidine incorporation compared with the untreated control group. Combined hCG and hMG therapy was effective for induction of spermatogenesis in patients with HH.
authors.affiliation: Department of Urology and Pathology, Osaka University Medical School, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Okuyama
authors.forename: N
authors.initials: N
authors.lastname: Nonomura
authors.forename: E
authors.initials: E
authors.lastname: Koh
authors.forename: N
authors.initials: N
authors.lastname: Kondoh
authors.forename: M
authors.initials: M
authors.lastname: Nakamura
authors.forename: M
authors.initials: M
authors.lastname: Namiki
authors.forename: H
authors.initials: H
authors.lastname: Kiyohara
authors.forename: Y
authors.initials: Y
authors.lastname: Nishimune
authors.forename: K
authors.initials: K
authors.lastname: Matsumoto
authors.forename: M
authors.initials: M
authors.lastname: Matsuda
mesh_terms: D000293:Adolescent; D000328:Adult; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D046508:Culture Techniques; D004247:DNA / Q000096:biosynthesis*; D004338:Drug Combinations; D005640:Follicle Stimulating Hormone / Q000097:blood; D006801:Humans; D007006:Hypogonadism / Q000188:drug therapy / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D008596:Menotropins / Q000494:pharmacology*; D013091:Spermatogenesis; D013737:Testis / Q000378:metabolism*; D013739:Testosterone / Q000097:blood; D013936:Thymidine / Q000378:metabolism
chemical_list: D006063:Chorionic Gonadotropin; D004338:Drug Combinations; D013739:Testosterone; D008596:Menotropins; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D004247:DNA; D013936:Thymidine