title: [Antibodies against extractable nuclear antigens. Clinico-biological correlations in 100 cases].
abstract: The clinical significance of antibodies directed against extractable nuclear antigens with currently well-known antigenic targets, is studied in 100 patients affected with various connective tissue inflammations. The anti-Sm is the most frequently encountered antibody in lupus disease and overlapping connective tissue inflammations with a lupus component while the anti-RNP is related to mixed inflammation of the connective tissue and the dry syndrome. These antibodies, without being specific markers for a given disease, represent an additional biological argument for a better nosological classification of connective tissue inflammations. The use of immuno-impression, a more sensitive technique than immunodiffusion, could provide a better understanding of the immune response during these diseases and specify the diagnostic and prognostic value of various auto-antibodies. Analysis by immuno-impression of 36 sera among the 100 with antibodies directed against extractable nuclear antigens has enabled us to compare the two techniques and study the different specificities of these antibodies.
authors.affiliation: Service de Médecine, Hôpital Charles-Nicolle, Tunis.
authors.forename: M R
authors.initials: MR
authors.lastname: Barbouch
authors.forename: Y
authors.initials: Y
authors.lastname: Gorgi
authors.forename: K
authors.initials: K
authors.lastname: Ayed
authors.forename: S
authors.initials: S
authors.lastname: Haddad
authors.forename: H
authors.initials: H
authors.lastname: Ben Ayed
mesh_terms: D000328:Adult; D000974:Antibodies, Antinuclear / Q000032:analysis*; D034961:Antigens, Nuclear; D001324:Autoantigens / Q000032:analysis*; D003095:Collagen Diseases / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D015151:Immunoblotting; D005779:Immunodiffusion; D008297:Male; D009687:Nuclear Proteins / Q000276:immunology*
chemical_list: D000974:Antibodies, Antinuclear; D034961:Antigens, Nuclear; D001324:Autoantigens; D009687:Nuclear Proteins
vernacular_title: Anticorps dirigés contre les antigènes nucléaires extractibles. Corrélations clinico-biologiques à propos de 100 cas.