Effective method for obtaining long nucleotide chains on partially complementary templates. Processed bovine gamma-interferon gene obtained from human gamma-interferon gene.
The method of obtaining the bovine gamma-interferon gene by means of simultaneous multidirected mutagenesis of the human gamma-interferon gene is presented. The first strand of the bovine gamma-interferon gene was obtained by ligation of synthetic oligonucleotides, using the cDNA of human gamma-interferon, cloned in the single-stranded phage M13mp19 as a template. The second strand was synthesized using a large fragment of E. coli DNA-polymerase I. The double-stranded gene was then treated by restriction nucleases and cloned in a pUC-18 derived vector. The primary structure was confirmed by sequencing.
Rostapshov
V M
VM
M.M. Shemyakin Institute of Bioorganic Chemistry, Moscow, USSR.
Chernov
I P
IP
Azhikina
T L
TL
Borodin
A M
AM
Sverdlov
E D
ED
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000138:chemical synthesis*; D012689:Sequence Homology, Nucleic Acid; D013698:Templates, Genetic
D009841:Oligonucleotides; D007371:Interferon-gamma