title: Cu(I)-thionein release from copper-loaded yeast cells.
abstract: The release of intact Cu(I)8-thionein from copper-resistant copper-loaded yeast cells, strain X2180-1Aa, has been shown. This copper(I)-thiolate-rich protein was characterized and compared with the chemical and physiocochemical properties of intracellular yeast Cu-thionein. The same molecular mass and stoichiometry of 8 mol copper atoms/mol protein was found. No detectable difference between the Cu-thioneins was seen in luminescence emission, electronic absorption in the ultraviolet region, chiroptical data or amino acid composition. The importance of stable Cu(I)-thiolates in Cu-thionein as a safe vehicle for transporting copper in a non-reactive manner is confirmed.
authors.affiliation: Physiologisch-Chemisches Institut, Universität Tübingen, Federal Republic of Germany.
authors.forename: K
authors.initials: K
authors.lastname: Felix
authors.forename: H J
authors.initials: HJ
authors.lastname: Hartmann
authors.forename: U
authors.initials: U
authors.lastname: Weser
mesh_terms: D002352:Carrier Proteins; D003300:Copper / Q000378:metabolism*; D008668:Metallothionein / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
chemical_list: C500769:CUP1-1 protein, S cerevisiae; D002352:Carrier Proteins; C007121:copper thionein; D003300:Copper; D008668:Metallothionein