Sequence, structure and evolution of the gene coding for sn-glycerol-3-phosphate dehydrogenase in Drosophila melanogaster.
We present the complete nucleotide and deduced amino acid sequence for the gene encoding Drosophila sn-glycerol-3-phosphate dehydrogenase. A transcription unit of 5kb was identified which is composed of eight protein encoding exons. Three classes of transcripts were shown to differ only in the 3'-end and to code for three protein isoforms each with a different C-terminal amino acid sequence. Each transcript is shown to arise through the differential expression of three isotype-specific exons at the 3'-end of the gene by a developmentally regulated process of 3'-end formation and alternate splicing pathways of the pre-mRNA. In contrast, the 5'-end of the gene is simple in structure and each mRNA is transcribed from the same promoter sequence. A comparison of the organization of the Drosophila and murine genes and the primary amino acid sequence between a total of four species indicates that the GPDH gene-enzyme system is highly conserved and is evolving slowly.
Bewley
G C
GC
Department of Genetics, North Carolina State University, Raleigh 27695-7614.
Cook
J L
JL
Kusakabe
S
S
Mukai
T
T
Rigby
D L
DL
Chambers
G K
GK
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D004331:Drosophila melanogaster / Q000235:genetics*; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics*; D007527:Isoenzymes / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D011817:Rabbits; D014158:Transcription, Genetic
D007527:Isoenzymes; D005993:Glycerolphosphate Dehydrogenase