title: DNA photoreactivating enzyme from human tissues.
abstract: Photoreactivating enzyme activity has been quantitated in human fetal skin, kidney, lung, liver, brain and intestine, and in neonatal human foreskin. In all the tissues examined there were at least two activities: one nominally greater than 10,000 Da, and one nominally less than 10,000 Da. Both can photolyze pyrimidine dimers in DNA using only light of wavelengths greater than 320 nm, thus excluding tryptophan-mediated dimer splitting as an important mechanism for these activities. The activities are inactivated by digestion with trypsin or pronase, and decreased partially or totally by heating to 65 degrees C. The activities from all six tissues, as well as that from neonatal foreskin, act catalytically in dimer photolysis. The properties of macromolecular size, heat lability, protease sensitivity and catalytic pyrimidine dimer photolysis by a non-tryptophan-mediated mechanism correspond to those of a true photoreactivating enzyme.
authors.affiliation: Biology Department, Brookhaven National Laboratory, Upton, NY 11973.
authors.forename: S E
authors.initials: SE
authors.lastname: Ogut
authors.forename: S M
authors.initials: SM
authors.lastname: D'Ambrosio
authors.forename: M
authors.initials: M
authors.lastname: Samuel
authors.forename: B M
authors.initials: BM
authors.lastname: Sutherland
mesh_terms: D004255:Deoxyribodipyrimidine Photo-Lyase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006801:Humans; D008190:Lyases / Q000378:metabolism*; D008970:Molecular Weight; D010777:Photochemistry; D011740:Pyrimidine Dimers / Q000378:metabolism / Q000528:radiation effects; D014018:Tissue Distribution; D014364:Tryptophan / Q000378:metabolism
chemical_list: D011740:Pyrimidine Dimers; D014364:Tryptophan; D008190:Lyases; D004255:Deoxyribodipyrimidine Photo-Lyase