Secretion by yeast of the zymogen form of Mucor rennin, an aspartic proteinase of Mucor pusillus, and its conversion to the mature form.
The Mucor rennin gene encoding a prepro-form of the fungal aspartic proteinase from Mucor pusillus was expressed under the control of the yeast GAL7 promoter in Saccharomyces cerevisiae. An inactive zymogen of the enzyme with the 44-amino-acid pro-sequence was identified in the medium during the initial stage of cultivation. Processing of the purified zymogen to the mature enzyme proceeded autocatalytically under the acidic conditions. The rate of processing was accelerated by an increase in the concentration of the zymogen or addition of the mature enzyme. The in vitro processing was inhibited by inhibitors for the aspartic proteinases. The zymogen with no proteinase activity due to a mutation at the active site residue, Asp, was still processed at a relatively slower rate in a wild-type strain of yeast, but no processing occurred in the pep4-3 mutant strain of S. cerevisiae deficient in yeast proteinase A. Thus, Mucor rennin is excreted in a form of zymogen, which is then processed in the yeast secretion pathway mainly by the autocatalytic proteolysis but, alternatively, by a proteinase of yeast.
Hiramatsu
R
R
Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo, Japan.
Aikawa
J
J
Horinouchi
S
S
Beppu
T
T
D000595:Amino Acid Sequence; D012085:Chymosin / Q000096:biosynthesis / Q000235:genetics*; D004792:Enzyme Precursors / Q000235:genetics; D005796:Genes; D005800:Genes, Fungal; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008969:Molecular Sequence Data; D009089:Mucor / Q000201:enzymology / Q000235:genetics*; D009154:Mutation; D010957:Plasmids; D011499:Protein Processing, Post-Translational; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*
D004792:Enzyme Precursors; C032443:prorennin; D012085:Chymosin