Proteoglycans synthesized by chondrocytes of human nonarthritic and osteoarthritic cartilage.
Chondrocyte cultures were developed from the cell outgrowths of explanted human nonarthritic and osteoarthritic human cartilage. Two significant differences in sulfated proteoglycan synthesis were demonstrated between the chondrocytes obtained in this manner. With 35SO4 to measure newly synthesized proteoglycan, we found that chondrocytes derived from osteoarthritic cartilage secreted significantly less (P less than 0.05) high density proteoglycan into the culture medium than did chondrocytes from nonarthritic cartilage after 20 hr of radiolabeling. This reduced amount of high density proteoglycan was sustained when chondrocytes were maintained in unlabeled culture medium ("chase" medium). In addition, the osteoarthritic chondrocytes secreted an increased amount of low density proteoglycan when compared with their nonarthritic counterparts. The elution profile of secreted high density proteoglycan isolated from the osteoarthritic chondrocyte culture medium was assessed by gel filtration on Sepharose CL-2B and revealed the presence of two proteoglycan subpopulations (Kav, 0.25, 0.58), whereas only one proteoglycan series (Kav, 0.37) was seen in the high density fraction of nonarthritic chondrocyte culture medium. Similar gel filtration profiles were also obtained when chondrocytes were maintained in chase medium. The results of this study demonstrated that stable differences in proteoglycan synthesis, but not in intracellular processing, exist between nonarthritic and osteoarthritic chondrocytes. The findings are noteworthy in that these differences were not previously apparent when organ-cultured cartilage was used to assess putative alterations in proteoglycans between the two groups.
Shuckett
R
R
Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106.
Malemud
C J
CJ
D000293:Adolescent; D000328:Adult; D000368:Aged; D002356:Cartilage / Q000378:metabolism*; D002478:Cells, Cultured; D002500:Centrifugation, Isopycnic; D002648:Child; D002675:Child, Preschool; D002808:Chondroitin Lyases / Q000378:metabolism; D002809:Chondroitin Sulfates / Q000032:analysis; D002850:Chromatography, Gel; D006801:Humans; D007700:Kinetics; D008875:Middle Aged; D010003:Osteoarthritis / Q000378:metabolism*; D011509:Proteoglycans / Q000032:analysis / Q000096:biosynthesis*; D013431:Sulfates / Q000378:metabolism
D011509:Proteoglycans; D013431:Sulfates; D002809:Chondroitin Sulfates; D002808:Chondroitin Lyases