title: Effect of active immunization against oxytocin on gonadotrophin secretion and the establishment of pregnancy in the ewe.
abstract: A group of 14 ewes was actively immunized against oxytocin coupled to carrier protein, and comparisons of the reproductive status of these animals were made against ewes immunized against carrier protein only (N = 5) and untreated controls (N = 6). The last two groups were indistinguishable and were therefore combined as a single control group for analysis of the results. Oestrous cycle lengths were significantly extended in oxytocin-immunized ewes (P less than 0.005) with 42% of cycles lasting greater than 18 days. Cloprostenol treatment in the mid-luteal phase resulted in apparently normal luteal regression and re-ovulation, but luteal phase FSH concentrations and follicular phase LH concentrations were elevated in the immunized ewes, although surge levels of both hormones were unaffected. Measurements of free oxytocin concentrations in the blood suggested that these were significantly raised in treated animals. Progesterone concentrations in peripheral plasma were not altered by treatment. Mating resulted in a conception rate of 91% in control ewes compared with only 28% in oxytocin-immunized animals (P less than 0.01). There was no evidence of any conceptus material in the uteri of non-pregnant immunized ewes 25 days after service. Some had re-ovulated, whereas the ovaries of others contained mature corpora lutea which had been maintained. Ovarian histology appeared normal. We conclude that active immunization against oxytocin influences gonadotrophin secretion and reduces fertility. The site(s) of action for both of these effects needs to be determined.
authors.affiliation: Department of Anatomy, Medical School, Bristol, UK.
authors.forename: D C
authors.initials: DC
authors.lastname: Wathes
authors.forename: V J
authors.initials: VJ
authors.lastname: Ayad
authors.forename: S A
authors.initials: SA
authors.lastname: McGoff
authors.forename: K L
authors.initials: KL
authors.lastname: Morgan
mesh_terms: D000818:Animals; D003008:Cloprostenol / Q000494:pharmacology; D004971:Estrus; D005260:Female; D005298:Fertility / Q000187:drug effects; D005640:Follicle Stimulating Hormone / Q000097:blood*; D007110:Immunity, Active; D007986:Luteinizing Hormone / Q000097:blood*; D010060:Ovulation; D010121:Oxytocin / Q000276:immunology*; D011247:Pregnancy; D012756:Sheep
chemical_list: D003008:Cloprostenol; D010121:Oxytocin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone