Specificity and antigenicity of mycoside G and other glycolipids from Mycobacterium marinum.
The purpose of this work was to examine the immunologic properties of the phenolicglycolipid produced by M. marinum (mycoside G). Cell mass processed with organic solvents yielded a lipid extract containing mycoside G and four other glycolipid fractions (G2 to G5). No mycoside G was found in the Mycobacterium marinum type strain. Neither mycoside G, nor fractions G2, G3 and G4 were immunogenic in the rabbit whereas fraction G5 was very immunogenic. The immune serum raised in the rabbit showed that fraction G5 is species specific since it was consistently detected in all Mycobacterium marinum strains examined so far.
Cruaud
P
P
Unité de la tuberculose et des mycobactéries, Institut Pasteur, Paris, France.
Papa
F
F
David
H L
HL
Daffé
M
M
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000302:isolation & purification*; D006017:Glycolipids / Q000276:immunology* / Q000302:isolation & purification; D007114:Immunization; D009161:Mycobacterium / Q000276:immunology*; D009170:Nontuberculous Mycobacteria / Q000276:immunology*; D011817:Rabbits; D013045:Species Specificity
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D006017:Glycolipids; C060732:mycoside G