title: Effect of a soluble bacterial carbohydrate fraction on the viscosity of intestinal contents in healthy subjects and patients with Crohn's disease.
abstract: Supernatants of faecal suspensions from patients with Crohn's disease (CD) showed much lower viscosity than those from healthy subjects. Material responsible for the viscosity could be precipitated with ethanol. Gel filtration indicated that the viscosity was not due to the glycoprotein fraction but to a fraction with higher molecular weight and relatively high contents of muramic acid suggesting a bacterial origin. The concentration and viscosity of this fraction are less in faeces from patients with CD than in that of healthy subjects.
authors.affiliation: Department of Immunology, Erasmus University, Rotterdam, The Netherlands.
authors.forename: M P
authors.initials: MP
authors.lastname: Hazenberg
authors.forename: A M
authors.initials: AM
authors.lastname: Pennock-Schröder
authors.forename: F
authors.initials: F
authors.lastname: Wensinck
authors.forename: J P
authors.initials: JP
authors.lastname: Van de Merwe
mesh_terms: D000328:Adult; D001421:Bacteria, Anaerobic / Q000302:isolation & purification; D011232:Chemical Precipitation; D002850:Chromatography, Gel; D003424:Crohn Disease / Q000382:microbiology*; D005243:Feces / Q000032:analysis* / Q000382:microbiology; D006801:Humans; D008875:Middle Aged; D009112:Muramic Acids / Q000032:analysis; D010457:Peptidoglycan / Q000032:analysis; D012794:Sialic Acids / Q000032:analysis; D014783:Viscosity
chemical_list: D009112:Muramic Acids; D010457:Peptidoglycan; D012794:Sialic Acids