Increased thermal stability of collagen after 2,5-hexanedione intoxication in rats.
The present study examined the ability of 2,5-hexanedione (2,5-HD) to cross-link the fibrillary protein, collagen, in vivo. The thermal stability of collagen measured as the isometric contraction-relaxation force was studied in tail tendons from rats, receiving 2,5-HD intraperitoneally for 2 or 6 weeks (1 g/kg/week, divided into 3 doses). The maximum contraction force was significantly increased after 6 weeks of intoxication as compared to controls, indicating an increase in covalent cross-linking between collagen molecules. Likewise, in vitro incubation of rat tail tendons in 2,5-HD for 24 hr induced an increase in thermal isometric contraction force.
Braendgaard
H
H
Department of Neurology, Diabetes Research Laboratory, Aarhus, Denmark.
Andreassen
T T
TT
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D003094:Collagen / Q000032:analysis*; D005976:Glutaral / Q000494:pharmacology; D006588:Hexanones / Q000506:poisoning*; D006358:Hot Temperature; D007537:Isometric Contraction / Q000187:drug effects; D007659:Ketones / Q000506:poisoning*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014867:Water / Q000032:analysis
D006588:Hexanones; D007659:Ketones; D014867:Water; D003094:Collagen; C011269:2,5-hexanedione; D005976:Glutaral