title: A concentration-dependent localization of octopamine-sensitive adenylate cyclase activity in locust skeletal muscle.
abstract: A concentration-dependent localization of octopamine-sensitive adenylate cyclase activity has been demonstrated in skeletal muscle of the locust, Schistocerca gregaria, using an histochemical technique. In the intermediate speed contracting muscle fibres from the fan region of the extensor-tibiae muscle of the locust hindleg, low concentrations of DL-octopamine (10(-8) M) induce reaction product preferentially in the sarcoplasmic reticular component of the dyads. At slightly higher concentrations (10(-7) and 10(-6) M) lower amounts of diffuse reaction product are also found in the non-dyad sarcoplasmic reticulum and at the sarcolemmal membrane, with occasional amounts of a less diffuse, punctuate product in the transverse tubule (T-tubule) component of the dyads. At higher concentrations (10(-5) and 10(-3) M) the predominant product is the dense, plaque-like accumulations of reaction product in the T-tubule component of the dyads. The results are discussed in terms of the likely physiological significance of the accumulation of reaction product in these different locations.
authors.affiliation: Department of Zoology, University of Cambridge, UK.
authors.forename: L S
authors.initials: LS
authors.lastname: Swales
authors.forename: P D
authors.initials: PD
authors.lastname: Evans
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D006110:Grasshoppers / Q000201:enzymology*; D006651:Histocytochemistry; D008297:Male; D008854:Microscopy, Electron; D009132:Muscles / Q000201:enzymology / Q000648:ultrastructure; D009655:Octopamine / Q000494:pharmacology*; D012519:Sarcoplasmic Reticulum / Q000201:enzymology
chemical_list: D009655:Octopamine; D000262:Adenylyl Cyclases