Bathophenanthroline-Ru(II) complexes as nonradioactive labels for dideoxy DNA sequencing.
Bathophenanthroline-Ru(II) complexes represent interesting nonradioactive label molecules which can be measured in a time-resolved mode with high sensitivity. Here we demonstrate that Ru complex-labeled primers can be applied in dideoxy sequencing protocols giving the same sequencing patterns as unlabeled primers.
Bannwarth
W
W
Central Research Units, F. Hoffmann-La Roche Ltd., Basel, Switzerland.
D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D015224:Dideoxynucleosides / Q000032:analysis*; D004492:Edetic Acid; D005063:Europium; D005452:Fluoresceins; D005453:Fluorescence; D008969:Molecular Sequence Data; D010618:Phenanthrolines; D012413:Rubidium / Q000032:analysis*; D013698:Templates, Genetic
D015224:Dideoxynucleosides; D005452:Fluoresceins; D010618:Phenanthrolines; D005063:Europium; C006686:bathophenanthroline; D004247:DNA; D004492:Edetic Acid; D012413:Rubidium