The ability of a ternary complex to form over the serum response element correlates with serum inducibility of the human c-fos promoter.
Rapid induction of c-fos transcription by serum and phorbol esters requires the serum response element (SRE). The SRE contains a 20 bp element of interrupted dyad symmetry (DSE) that is bound by p67/SRF. We have identified a hitherto unrecognized protein with an apparent size of 62 kd. This novel component, p62, is shown to be an integral but physically separable part of a ternary complex formed with p67/SRF and the SRE. Alone, p62 fails to bind the SRE but requires DSE-bound p67/SRF and sequences both within and outside the DSE for its interaction with DNA. In vivo, the response of the c-fos promoter to serum is severely impaired by mutations that abolish ternary complex formation in vitro.
Shaw
P E
PE
Zentrum für Molekulare Biologie, Universität Heidelberg, Federal Republic of Germany.
Schröter
H
H
Nordheim
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A
D001483:Base Sequence; D002460:Cell Line; D003470:Culture Media; D004268:DNA-Binding Proteins / Q000502:physiology*; D005786:Gene Expression Regulation; D046911:Macromolecular Substances; D008745:Methylation; D008970:Molecular Weight; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016760:Proto-Oncogene Proteins c-fos; D012045:Regulatory Sequences, Nucleic Acid; D013329:Structure-Activity Relationship; D014157:Transcription Factors / Q000502:physiology*; D014158:Transcription, Genetic
D003470:Culture Media; D004268:DNA-Binding Proteins; D046911:Macromolecular Substances; D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D014157:Transcription Factors