Polycarbonate membranes: a novel surface for solid-phase determinations with utility in field format serological assays.
A dipstick suitable for immunoassay procedures has been developed, and its utility demonstrated by the development of tests for the determination of immunoglobulin levels in newborn bovine blood, and for the detection of bovine antibodies against Brucella abortus. The dipstick is of simple design, consisting of three components: a polycarbonate membrane, an adhesive, and a support material. The polycarbonate membrane is a porous filter material upon which the immunoassay is undertaken. Biomolecules bind to the membrane with sufficient affinity to permit the development of useful immunoassay procedures; however, this membrane exhibits low non-specific binding of reagents. Collectively, these properties make polycarbonate a useful material for solid-phase immunoassay procedures.
Penney
C L
CL
Agritech Research Inc., Burlington, Ontario, Canada.
Gauldie
J
J
Evelegh
M
M
Penney
M T
MT
Chong
D
D
Horsewood
P
P
D000818:Animals; D000831:Animals, Newborn; D000907:Antibodies, Bacterial / Q000032:analysis; D002003:Brucella abortus / Q000276:immunology; D002006:Brucellosis / Q000175:diagnosis; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000276:immunology; D003738:Dental Cements; D007136:Immunoglobulins / Q000032:analysis; D007163:Immunosorbent Techniques / Q000295:instrumentation*; D008567:Membranes, Artificial; D011076:Polycarboxylate Cement
D000907:Antibodies, Bacterial; D003738:Dental Cements; D007136:Immunoglobulins; D008567:Membranes, Artificial; D011076:Polycarboxylate Cement; C005506:polycarbonate