[Preparation and characteristics of a new transformed cell line G11 by transfection of NIH/3T3 mouse fibroblasts with DNA from the SA7 oncogene of simian adenovirus].
Murine fibroblasts NIH 3T3 were transfected with the plasmid pASP containing simian adenovirus oncogene insertion. Focus forming transformants were cloned with a final dilution technique and a new cell line G11 was created as a result. Transformed status of this cell line is evidenced by changes in morphology, specific cytochemical and adhesion properties, ability to grow in semisolid agar and FCS concentration growth independence. Presence of intact integrated E1a-region of adenovirus SA7 oncogene was shown by blot-hybridization technique. Transformed status of G11 cells can be explained by integration of SA7 oncogene, that is evidenced indirectly by the increased resistance to heat shock.
Solov'ev
G Ia
GIa
Pantin
V I
VI
Surin
V L
VL
Zhukova
E L
EL
Kotel'nikov
V M
VM
D000256:Adenoviridae / Q000235:genetics*; D000261:Adenoviruses, Simian / Q000235:genetics*; D000818:Animals; D002461:Cell Line, Transformed; D004279:DNA, Viral / Q000235:genetics*; D005347:Fibroblasts; D005814:Genes, Viral; D000882:Haplorhini; D006358:Hot Temperature; D051379:Mice; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D010957:Plasmids; D014162:Transfection
D004279:DNA, Viral
Poluchenie i kharakteristika novoĭ transformirovannoĭ kletochnoĭ linii G11 pri transfektsii fibroblastov mysheĭ NIH/3T3 DNK onkogena adenovirusa obez'ian SA7.