title: [In vitro sensitivity of 3 strains of Leptospira interrogans to 3 different antibiotics].
abstract: Leptospirosis, anthropo-zoonosis ubiquitously widespread, is a social and economic problem still to be solved. The experimental and therapeutic employment of many antibiotics has largely been tested "in vitro" and "in vivo". In the following research we tried to evaluate, by experimental "in vitro" method, the sensitivity difference of three serovar strains of Leptospira interrogans to two macrolides, Erythromycin and Josamycin, compared with Penicillin. From standard cultures, previously treated with serial dilution of these antibiotics, the MIC and MSC, as quantitative parameters, have been stated. For the qualitative evaluation of the damages induced at ultrastructural level by the drug activity. Electron Microscopy investigations were performed on specimens from cultures treated for 6 hr with twice and tenfold the MSC. The present research confirms the good sterilizing efficaciousness "in vitro" of the tested macrolides (MSC less than 1 mcg/ml) and their different activity pathway.
authors.affiliation: Istituto di Clinica delle Malattie Infettive, Università di Pavia, IRCCS Ospedale Policlinico San Matteo.
authors.forename: R
authors.initials: R
authors.lastname: Benzi Cipelli
authors.forename: G
authors.initials: G
authors.lastname: Gorini
authors.forename: S
authors.initials: S
authors.lastname: Caligaris
authors.forename: P
authors.initials: P
authors.lastname: Lanzarini
authors.forename: F
authors.initials: F
authors.lastname: Milano
authors.forename: P
authors.initials: P
authors.lastname: Orsolini
authors.forename: G
authors.initials: G
authors.lastname: Filice
mesh_terms: D004352:Drug Resistance, Microbial; D004917:Erythromycin / Q000494:pharmacology*; D015570:Josamycin / Q000494:pharmacology*; D007921:Leptospira interrogans / Q000145:classification / Q000187:drug effects* / Q000648:ultrastructure; D010403:Penicillin Resistance; D010406:Penicillins / Q000494:pharmacology*; D013045:Species Specificity
chemical_list: D010406:Penicillins; D004917:Erythromycin; D015570:Josamycin
vernacular_title: Suscettibilità in vitro di tre ceppi di Leptospira interrogans a tre differenti antibiotici.