[Use of electroblotting as a method for preparing samples of proteins and their fragments for microsequencing].
Gel electrophoresis in the presence of sodium dodecyl sulphate followed by electroblotting was employed in sample preparation for microsequencing proteins and protein fragments. Three types of solid supports were compared: glass fiber filters modified by aminopropyltriethoxysilane or covered with polybrene, and polyvinylidenedifluoride membranes. N-Terminal amino acid sequences of several proteins (Mr 14-140 kDA were determined on a gas-phase sequencer with the standard programme; 20-200 pmoles of the protein can be assayed by this method.
Levina
N B
NB
Slepak
V Z
VZ
Kiselev
O G
OG
Shemiakin
V V
VV
Khokhlachev
A A
AA
D000262:Adenylyl Cyclases / Q000032:analysis*; D000595:Amino Acid Sequence; D019204:GTP-Binding Proteins / Q000032:analysis*; D015151:Immunoblotting; D010449:Peptide Mapping / Q000379:methods*; D010455:Peptides / Q000032:analysis*
D010455:Peptides; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases
Primenenie élektroblottinga v kachestve metoda polucheniia obraztsov belkov i ikh fragmentov dliia mikrosekvenirovaniia.