Protein extraction with reverse micelles.
Liquid-liquid protein extraction with reverse micelles is a new method for protein separation. We studied the effects of pH, ionic concentration, cation type, and protein molecular mass on performance of an AOT/isooctane reverse micelle system in extraction of lysozyme, trypsin, and pepsin. Extraction was found to be very efficient for low-molecular-mass proteins.
Shi
H Q
HQ
Department of Chemical Engineering, Tsinghua University, Beijing.
Xia
L
L
Guo
R
R
Shen
Z Y
ZY
D002412:Cations; D006863:Hydrogen-Ion Concentration; D008722:Methods; D008823:Micelles; D009113:Muramidase / Q000302:isolation & purification; D009994:Osmolar Concentration; D010434:Pepsin A / Q000302:isolation & purification; D011489:Protein Denaturation; D011506:Proteins / Q000302:isolation & purification*; D012492:Salts; D014357:Trypsin / Q000302:isolation & purification
D002412:Cations; D008823:Micelles; D011506:Proteins; D012492:Salts; D009113:Muramidase; D014357:Trypsin; D010434:Pepsin A