Effect of selected denervations on glycoconjugate composition and tissue morphology during the initiation phase of limb regeneration in adult Ambystoma.
This study was undertaken to assess the effects of various quantities of neural tissue on the temporal relationship of matrix glycoconjugates to the regeneration morphology. 1) Denervation before amputation revealed that a threshold level of nervous tissue was necessary to activate a regeneration response from the tissue, i.e., appearance of regeneration-specific morphologies and glycoconjugates. 2) Denervation after amputation demonstrated that the level of neural tissue necessary to maintain these responses was below the level necessary to activate the regeneration response. If neural tissue was completely removed there was a concomitant loss of regenerate morphologies and glycoconjugates. 3) Bilateral amputation of a neurogenically intact limb and its completely denervated contralateral limb revealed that the regeneration response was a localized phenomenon during the first 30 days after amputation. After 30 days the regeneration response appeared within the previously degenerated denervating limb. The results suggest that the factors controlling the regenerative response in adult Ambystoma are large diffusible substances that can be transported by the circulation and can affect the regenerative response in remote, previously activated, tissues.
Young
H E
HE
Department of Anatomy, Texas Tech University Health Sciences Center, Lubbock 79430.
Dalley
B K
BK
Markwald
R R
RR
D000557:Ambystoma / Q000502:physiology*; D000818:Animals; D002809:Chondroitin Sulfates / Q000032:analysis; D003714:Denervation; D005121:Extremities / Q000032:analysis / Q000166:cytology / Q000502:physiology*; D006001:Glycoconjugates / Q000032:analysis*; D006023:Glycoproteins / Q000032:analysis; D006497:Heparan Sulfate / Q000032:analysis; D006820:Hyaluronic Acid / Q000032:analysis; D012038:Regeneration
D002809:Chondroitin Sulfates; D006001:Glycoconjugates; D006023:Glycoproteins; D006497:Heparan Sulfate; D006820:Hyaluronic Acid