title: Canine pythiosis--isolation and identification of Pythium insidiosum.
abstract: Pythium insidiosum was isolated from the subcutaneous tissue of a 1-year-old tan crossbreed dog and from the intestinal tract of an 18-month-old Samoyed male. Gomori's methenamine silver stain was superior to hematoxylin and eosin in demonstrating the organism in tissue sections. The agent was identified as P. insidiosum by zoospore formation in an aqueous yeast extract solution containing grass blades. Exoantigens produced in culture were shown to be identical to known P. insidiosum antigens by microimmunodiffusion.
authors.affiliation: College of Veterinary Medicine, Drawer V, Mississippi State University, MS 39762.
authors.forename: J
authors.initials: J
authors.lastname: Bentinck-Smith
authors.forename: A A
authors.initials: AA
authors.lastname: Padhye
authors.forename: W R
authors.initials: WR
authors.lastname: Maslin
authors.forename: C
authors.initials: C
authors.lastname: Hamilton
authors.forename: R K
authors.initials: RK
authors.lastname: McDonald
authors.forename: B J
authors.initials: BJ
authors.lastname: Woody
mesh_terms: D000818:Animals; D003881:Dermatomycoses / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D004283:Dog Diseases / Q000382:microbiology*; D004285:Dogs; D007410:Intestinal Diseases / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D008297:Male; D009181:Mycoses / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D011775:Pythium / Q000302:isolation & purification*; D013172:Spores, Fungal