[Isolation of monoclonal antibodies for the identification of strains of the causative agent of plague that do not contain a capsular antigen].
In most cases the immunological identification of Y. pestis strains is based on the use of capsular antigen as an immunological marker. However, there are Y. pestis strains without capsular antigen. For the immunological identification of such strains, homogeneous antigen with a molecular weight of 43 KD has been isolated and monoclonal antibodies to it have been obtained. The enzyme-linked immunosorbent assay, carried out with the use of these monoclonal antibodies and intended for the detection of antigen with a molecular weight of 43 KD, has been developed. The sensitivity of the assay is about 10 ng/ml.
Zaĭtsev
A A
AA
Vikhrev
N E
NE
Sazykin
A Iu
AIu
Vengerova
Iu Iu
IuIu
Levi
M I
MI
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000302:isolation & purification*; D000942:Antigens, Bacterial / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D006386:Hemagglutination Tests; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D005779:Immunodiffusion; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D010930:Plague / Q000175:diagnosis; D013997:Time Factors; D015010:Yersinia pestis / Q000276:immunology*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial
Poluchenie monoklonal'nykh antitel dlia identifikatsii shtammov vozbuditelia chumy, ne soderzhashchikh kapsul'nogo antigena.