title: Pyruvate oxidation in vivo and in vitro in Dictyostelium discoideum.
abstract: The in vivo rate of the reaction catalyzed by the pyruvate dehydrogenase complex in Dictyostelium discoideum was measured by exposing cells to [1-14C]alanine and relating the rate of 14CO2 evolution to the specific radioactivity of the isolated [1-14C]pyruvate. The rate obtained, 0.33 mumol/min per ml packed cell volume, was very similar to that determined by independent calculations based upon the rate of utilization of endogenous protein as an energy source in this system.
authors.affiliation: Microbiology Department, University of Montana, Missoula 59812.
authors.forename: M H
authors.initials: MH
authors.lastname: Butler
authors.forename: B E
authors.initials: BE
authors.lastname: Wright
mesh_terms: D000409:Alanine / Q000378:metabolism; D002245:Carbon Dioxide / Q000032:analysis; D002250:Carbon Radioisotopes; D004023:Dictyostelium / Q000201:enzymology*; D010084:Oxidation-Reduction; D011768:Pyruvate Dehydrogenase Complex / Q000378:metabolism*; D011773:Pyruvates / Q000378:metabolism*; D011865:Radioisotope Dilution Technique
chemical_list: D002250:Carbon Radioisotopes; D011768:Pyruvate Dehydrogenase Complex; D011773:Pyruvates; D002245:Carbon Dioxide; D000409:Alanine