Autoregulation of the ccd operon in the F plasmid.
Mini-F sequences, including the promoter and portions of the ccd region, were inserted upstream of lacZ in promoterless lacZ vectors, and beta-galactosidase specific activities were measured. The results showed that the H (ccdA), G (ccdB) and D genes, together with a promoter, comprise an operon. Ccd operon expression was shown to be regulated at the level of transcription by the G gene product, probably in concert with the H gene product. Thus expression is autoregulated. Expression of the D gene was largely dependent on the ccd promoter, although low levels of transcription from another promoter within the ccd coding region were detected.
de Feyter
R
R
Department of Cellular and Molecular Biology, University of Auckland, New Zealand.
Wallace
C
C
Lane
D
D
D004926:Escherichia coli / Q000235:genetics*; D005144:F Factor; D005786:Gene Expression Regulation; D009876:Operon; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000378:metabolism
D001616:beta-Galactosidase