The detection and enumeration of cytokine-secreting cells in mice and man and the clinical application of these assays.
An in vitro assay for the detection and enumeration of mouse and human cytokine-secreting cells is described and some ways in which it may be used diagnostically are indicated. The assay is an extension of the ELISA plaque assay or ELISPOT assay and uses pairs of antibodies to capture and then visually develop secreted lymphokines. In this way, it is possible to enumerate the specific cytokine-secreting cells. This assay may provide a valuable tool in the clinical investigation of the mechanisms of disease development and tissue destruction.
Hutchings
P R
PR
Department of Immunology, University College and Middlesex School of Medicine, London, U.K.
Cambridge
G
G
Tite
J P
JP
Meager
T
T
Cooke
A
A
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001685:Biological Factors / Q000378:metabolism*; D002478:Cells, Cultured; D016207:Cytokines; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007371:Interferon-gamma / Q000032:analysis; D008222:Lymphokines / Q000032:analysis*; D051379:Mice; D009132:Muscles / Q000166:cytology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D000911:Antibodies, Monoclonal; D001685:Biological Factors; D016207:Cytokines; D008222:Lymphokines; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma