[Study of in vitro OK432-induced IFN-gamma production in patients with gastric and esophageal cancers: comparison with in vitro skin tests].
In the present study, we measured in vitro production of IFN-gamma induced by OK432 in peripheral mononuclear cells (PMC) and investigated the relationship between the skin reaction to Su-Ps, an extract of Streptococcus Pyogenes, and purified protein derivative (PPD) and the productibility of IFN-gamma in patients with gastric and esophageal cancers. PMC isolated with the Ficoll-conray centrifugation technique were adjusted to 10(6) cells/ml in RPMI-1640 medium supplemented with 10% fetal calf serum and incubated over 7 days at 37 degrees C in the presence of OK432 at 0.17 KE/ml in microculture plates. IFN-gamma secreted in the supernatants was measured consecutively during the observation period with radioimmunoassay. The results are summarized as follows. 1. The production of IFN-gamma in PMC from patients with negative skin reaction to Su-Ps and PPD was significantly decreased as compared with the value obtained from patients who were positive in these skin tests (p less than 0.001). 2. In vitro production of IFN-gamma induced by OK432 was significantly correlated with the degree of skin tests to Su-Ps and PPD as well (r = 0.48, r = 0.41, p less than 0.01, respectively). Thus, it is concluded that the assay of IFN-gamma produced in PMC cultures is useful to evaluate the immunological status of patients with cancer.
Matsumoto
H
H
2nd Department of Surgery, Kinki University School of Medicine, Osakasayama, Japan.
Izutani
R
R
Terada
M
M
Noguchi
J
J
Shiraha
S
S
Kuyama
T
T
Aoki
N
N
D001426:Bacterial Proteins; D001688:Biological Products / Q000494:pharmacology*; D004938:Esophageal Neoplasms / Q000276:immunology* / Q000378:metabolism; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008934:Mitogens / Q000494:pharmacology; D010844:Picibanil / Q000494:pharmacology*; D012882:Skin Tests / Q000379:methods; D013274:Stomach Neoplasms / Q000276:immunology* / Q000378:metabolism; D014374:Tuberculin Test; D014407:Tumor Cells, Cultured
D001426:Bacterial Proteins; D001688:Biological Products; D008934:Mitogens; C034866:streptococcal group A mitogen; D010844:Picibanil; D007371:Interferon-gamma