Influence of thyroidal status on pituitary content of thyrotropin beta- and alpha-subunit, growth hormone, and prolactin messenger ribonucleic acids.
Thyroidectomized rats were used to study the effects of a single injection of T3 on pituitary mRNA synthesis and hormone secretion. T3 was injected ip at doses of 0, 0.2, 1, or 5 micrograms/100 g body weight, and and animals were killed 24 h later. T3 caused a significant decrease in serum TSH, but caused no significant change in either serum GH or PRL. Pituitary mRNA was quantified by slot blot hybridization with cDNA probes specific for alpha-TSH, beta-TSH, PRL, and GH. We found that both the alpha and beta mRNA subunits decreased, that PRL mRNA remained relatively unchanged, and that GH mRNA increased with increasing T3 dose. The data show that a single dose of T3 can profoundly influence mRNA levels in the anterior pituitary; the lowest dose of T3 caused maximum inhibition of alpha-TSH mRNA while beta-TSH mRNA declined further in a dose-dependent manner.
Mirell
C J
CJ
Endocrinology Research Laboratory, Wadsworth Veterans Administration Medical Center, Los Angeles, California 90073.
Yanagisawa
M
M
Lau
R
R
Pekary
A E
AE
Chin
W W
WW
Hershman
J M
JM
D000818:Animals; D015292:Glycoprotein Hormones, alpha Subunit / Q000235:genetics*; D013006:Growth Hormone / Q000235:genetics*; D008297:Male; D009693:Nucleic Acid Hybridization; D010903:Pituitary Gland, Anterior / Q000187:drug effects / Q000378:metabolism*; D011388:Prolactin / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification; D051381:Rats; D011919:Rats, Inbred Strains; D013965:Thyroidectomy; D013972:Thyrotropin / Q000235:genetics*; D014284:Triiodothyronine / Q000494:pharmacology
D015292:Glycoprotein Hormones, alpha Subunit; D012333:RNA, Messenger; D014284:Triiodothyronine; D011388:Prolactin; D013972:Thyrotropin; D013006:Growth Hormone