title: Reaction on alternating GC oligonucleotide templates.
abstract: The condensation reactions of activated nucleotides, ImpN or 2-MeImpN, with the self-complementary ribo-octanucleotide GCGCGCGC or with the partially self-complementary heptanucleotide GCGCGCG were studied. The template-directed reaction of 2-MeImpC with the heptamer yields the 3'-5' octamer as the main product. All other reactions yield 2'-5'-linked octamers and pyrophosphates as major products. Surprisingly, 2-MeImpG facilitates the reaction of 2-MeImpC with the heptamer. Procedures for the analysis by gel electrophoresis of the oligomeric products obtained in reactions of this kind are described.
authors.affiliation: Salk Institute for Biological Studies, San Diego, California 92138.
authors.forename: K E
authors.initials: KE
authors.lastname: Ng
authors.forename: L E
authors.initials: LE
authors.lastname: Orgel
mesh_terms: D001345:Autoradiography; D001483:Base Sequence; D007202:Indicators and Reagents; D009843:Oligoribonucleotides / Q000138:chemical synthesis; D010761:Phosphorus Radioisotopes; D013329:Structure-Activity Relationship; D013698:Templates, Genetic
chemical_list: D007202:Indicators and Reagents; D009843:Oligoribonucleotides; D010761:Phosphorus Radioisotopes