title: K-252a specifically inhibits the survival and morphological differentiation of NGF-dependent neurons in primary cultures of human dorsal root ganglia.
abstract: We have measured neurofilament protein expression in cultures of human dorsal root ganglia (DRG) grown in both the presence and absence of nerve growth factor (NGF). Cultures established from DRG of 8-13 weeks gestation contained populations of both NGF-dependent and -independent neurofilament-positive cells. A recently described kinase inhibitor named K-252a inhibited NGF-dependent increases in neurofilament protein expression with half-maximal and maximal inhibition apparent at concentrations of 6 and 25 nM. At up to 50 nM, K-252a had no effect on neurofilament protein expression from NGF-independent neurons.
authors.affiliation: Institute of Neurology, London, U.K.
authors.forename: P
authors.initials: P
authors.lastname: Doherty
authors.forename: F S
authors.initials: FS
authors.lastname: Walsh
mesh_terms: D002227:Carbazoles / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005727:Ganglia, Spinal / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D026121:Indole Alkaloids; D007381:Intermediate Filament Proteins / Q000378:metabolism; D009414:Nerve Growth Factors / Q000494:pharmacology*; D016900:Neurofilament Proteins
chemical_list: D002227:Carbazoles; D026121:Indole Alkaloids; D007381:Intermediate Filament Proteins; D009414:Nerve Growth Factors; D016900:Neurofilament Proteins; C049985:staurosporine aglycone