[UV-inducibility of the LT-toxin operon].
The plasmid elt-operon pVZ14 was constructed by fusing of the eltoperon of the plasmid pVZ357 with the lac-gene of the bacteriophage Mud1 (Amp, Lac). lacZ gene has been proven to be fused with an elt-promoter by the loss of toxin production coded by pVZ357 and acquiring of Lac+ phenotype by pVZ14 containing cells, as well as by HindIII fragments hybridization of pVZ357 and pVZ14 with the labelled elt-probe. The kinetics of beta-galactosidase synthesis in E. coli cells harboring pVZ14 shows an elt-operon promoter to have expressed constitutive activity and to be activated by a SOS-inducing agent, UV-light.
Tiganova
I G
IG
Rusina
O Iu
OIu
Andreeva
I V
IV
Demkin
V V
VV
Brukhanskiĭ
G V
GV
Aleshkin
G I
GI
Skavronskaia
A G
AG
D001345:Autoradiography; D001427:Bacterial Toxins / Q000235:genetics*; D015139:Blotting, Southern; D002478:Cells, Cultured; D004768:Enterotoxins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D009876:Operon / Q000528:radiation effects*; D010957:Plasmids; D015183:Restriction Mapping; D014466:Ultraviolet Rays; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000378:metabolism
D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C027538:heat-labile enterotoxin, E coli; D001616:beta-Galactosidase
UF-indutsibel'nost' operona LT-toksina.