title: Neuropeptide degradation by large vessel and microvessel-derived endothelial cells in vitro: cell surface catabolism of thyrotropin releasing hormone (TRH).
abstract: Cell surface ectopeptidase activity of purified, cultured large vessel and microvessel-derived endothelial cells (EC) was studied. Degradation of thyrotropin releasing hormone (TRH), and production of cyclo-His-Pro was significantly increased (P less than 0.001) in large vessel EC compared with microcapillary EC. Since the rate of catabolism in the microvascular capillary bed is 5 times less than that in the large vessel wall, peptide concentrations are likely maintained longer in close proximity to their site of biosynthesis, where they are presumably most active.
authors.affiliation: Neurology Service, Wm. S. Middleton Veterans Administration Hospital, Madison, WI 53705.
authors.forename: J M
authors.initials: JM
authors.lastname: Rozental
authors.forename: G
authors.initials: G
authors.lastname: Kaminska
authors.forename: J
authors.initials: J
authors.lastname: Turner
authors.forename: T
authors.initials: T
authors.lastname: Schwartz
authors.forename: V
authors.initials: V
authors.lastname: Cadahia
authors.forename: B R
authors.initials: BR
authors.lastname: Brooks
mesh_terms: D000626:Aminopeptidases / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009479:Neuropeptides / Q000378:metabolism*; D013973:Thyrotropin-Releasing Hormone / Q000378:metabolism*
chemical_list: D009479:Neuropeptides; D013973:Thyrotropin-Releasing Hormone; D000626:Aminopeptidases