title: T lymphocytes and monocytes bind to keratinocytes in frozen sections of biopsy specimens of normal skin treated with gamma interferon.
abstract: When 2 mm punch biopsy specimens of normal skin are incubated with gamma interferon (IFN-gamma), the keratinocytes are induced to express intercellular adhesion molecule-1 (ICAM-1). Lymphocytes and monocytes that express lymphocyte function-associated antigen-1 (LFA-1) bind to cultured keratinocytes expressing ICAM-1. We have developed an in vitro adherence assay using frozen sections of normal skin that have been incubated with IFN-gamma, and then overlaid by peripheral blood mononuclear leukocytes. Although peripheral blood mononuclear leukocytes do not bind to the epidermal keratinocytes of untreated skin sections, after exposure of the skin to IFN-gamma the peripheral blood mononuclear leukocytes prominently bind to the epidermal keratinocytes that express ICAM-1. The binding by peripheral blood mononuclear leukocytes is increased approximately twofold by activation with phorbol ester treatment. The temperature dependence and kinetics of this adherence reaction reveal no binding at 8 degrees C, good binding at 24 degrees C, and optimal binding at 37 degrees C, reaching a maximal extent by 60 minutes. The adherence reaction is blocked either by pretreating the peripheral blood mononuclear leukocytes with LFA-1 antibody or the IFN-gamma-exposed skin specimen with ICAM-1 antibody. The immunophenotypic analysis of the adherent peripheral blood mononuclear leukocytes to epidermal keratinocytes revealed that most cells activated by 12-O-tetradecanoyl-phorbol-13-acetate are T lymphocytes, with CD8+ T cells binding slightly better than CD4+ T cells, with a smaller population of monocytes. These results provide additional support for a role of LFA-1, ICAM-1, and IFN-gamma in modulating keratinocyte-lymphocyte interactions.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Pathology, University of Michigan Medical Center, Ann Arbor.
authors.forename: B J
authors.initials: BJ
authors.lastname: Nickoloff
authors.forename: C E
authors.initials: CE
authors.lastname: Griffiths
mesh_terms: D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000096:biosynthesis; D000954:Antigens, Surface / Q000096:biosynthesis; D001706:Biopsy; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules; D002478:Cells, Cultured; D046508:Culture Techniques; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism; D005629:Frozen Sections; D006801:Humans; D007124:Immunoenzyme Techniques; D007371:Interferon-gamma / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D016169:Lymphocyte Function-Associated Antigen-1; D013601:T-Lymphocytes / Q000378:metabolism*; D013696:Temperature
chemical_list: D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D016169:Lymphocyte Function-Associated Antigen-1; D007371:Interferon-gamma