Selection and analysis of amino acid sequence of an antigenic domain of the rabies virus glycoprotein.
A new procedure for the selection of antigenic determinant of rabies virus glycoprotein is described. The glycoprotein was purified by polyacrylamide gel electrophoresis (PAGE). The glycoprotein fragments digested with protease V8 were separated by 25% PAGE, transferred onto nitrocellulose membrane, immunostained with monoclonal antibodies (McAb) against glycoprotein. One McAb-positive fragment (G-V-6) was obtained by using the Western blot analysis. The amino acid sequence of the fragment was analysed. The data indicated that the antigenic determinant of glycoprotein was located in a region between residues 130-141. These suggest that the Western blot analysis will be a useful tool for the development of synthetic vaccines.
Wang
H H
HH
Dietzschold
B
B
Koprowski
H
H
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D000939:Epitopes; D006023:Glycoproteins / Q000032:analysis / Q000276:immunology*; D006801:Humans; D011820:Rabies virus / Q000276:immunology*
D000939:Epitopes; D006023:Glycoproteins