Staphylococcus aureus tetrapeptide with high chemotactic potency and efficacy for human leukocytes.
A chemotactic tetrapeptide from culture fluids of Staphylococcus aureus was purified to homogeneity by reverse-phase high-pressure liquid chromatography. The peptide comprises equimolar methionine, leucine, isoleucine, and phenylalanine. It inhibited binding of fluoresceinated fMet-Leu-Phe-Lys to human monocytes, which showed that it interacted with the formyl-methionyl peptide receptor and suggested that it was a formyl-methionyl peptide. Based on a comparison of dose-response curves for inhibition of fluoresceinated fMet-Leu-Phe-Lys binding, the relative affinity of the peptide for the receptor was comparable to that of fMet-Leu-Phe-Lys. At optimal concentrations, chemotactic efficacy (percentage of monocytes migrating to the attractant) was 53 +/- 4%, in contrast to 36 +/- 3% for the reference attractant fMet-Leu-Phe. Since approximately 60% of human monocytes have formyl-peptide receptors, the bacterial peptide is capable of attracting all receptor-bearing monocytes.
Rot
A
A
Immunopathology Section, National Cancer Institute, Frederick, Maryland 21701.
Henderson
L E
LE
Sowder
R
R
Leonard
E J
EJ
D000596:Amino Acids / Q000032:analysis; D001667:Binding, Competitive; D002630:Chemotactic Factors / Q000302:isolation & purification / Q000502:physiology*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D006801:Humans; D009000:Monocytes / Q000378:metabolism / Q000502:physiology; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000031:analogs & derivatives / Q000378:metabolism; D009842:Oligopeptides / Q000302:isolation & purification / Q000502:physiology*; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic / Q000187:drug effects; D013211:Staphylococcus aureus / Q000032:analysis / Q000502:physiology*; D013861:Thiocyanates
D000596:Amino Acids; D002630:Chemotactic Factors; D005452:Fluoresceins; D009842:Oligopeptides; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic; D013861:Thiocyanates; C058574:chemotactic tetrapeptide; D009240:N-Formylmethionine Leucyl-Phenylalanine; C049813:N-formylmethionyl-leucyl-phenylalanyl-lysine; D016650:Fluorescein-5-isothiocyanate