Primary structure of an N-linked sugar chain derived from glucoamylase of Rhizopus niveus.
The primary structure of the N-linked sugar chain of Rhizopus niveus glucoamylase (major component) was investigated. The carbohydrate moiety was released from the polypeptide backbone by Flavobacterium sp. endo-beta-N-acetylglucosaminidase digestion. Studies using the method of exoglycosidase digestion of the fluorescent pyridylamino derivative, gel-permeation chromatography on Bio-Gel P-4 and 400-MHz 1H-NMR spectroscopy revealed that the most abundant structure is (Man)8-GlcNac-ol.
Takegawa
K
K
Department of Bioresource Science, Kagawa University, Japan.
Kawasaki
N
N
Iwahara
S
S
Yamamoto
K
K
Tochikura
T
T
Mikami
B
B
Morita
Y
Y
D000631:Aminopyridines; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005087:Glucan 1,4-alpha-Glucosidase / Q000032:analysis / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008358:Mannose / Q000032:analysis; D008361:Mannosidases / Q000378:metabolism; D010084:Oxidation-Reduction; D012233:Rhizopus / Q000201:enzymology*
D000631:Aminopyridines; D006026:Glycoside Hydrolases; D008361:Mannosidases; D005087:Glucan 1,4-alpha-Glucosidase; D008358:Mannose; C032439:alpha-aminopyridine