title: Excretion of acetylated and free polyamines by polyamine depleted Chinese hamster ovary cells.
abstract: 1. Cultured Chinese hamster ovary cells (CHO) and their ornithine decarboxylase deficient mutant cells (C55.7) were found to excrete small amounts of N8-acetylspermidine and free polyamines, putrescine and spermidine into the culture medium. 2. The concentration of N8-acetylspermidine in the control cells was 2-3% of that of spermidine. In the medium, however, the amount of N8-acetylspermidine was about 2-fold that of spermidine and 2- to 3-fold higher than the intracellular amount. N1-acetylspermidine or acetylated spermine were never detected in the cells or in the media. 3. Confluent CHO cells treated with 2 mM difluoromethylornithine stopped the excretion when the intracellular spermidine concentration had decreased to 20% of control while there was no decrease in spermine concentration. At low cell density, neither polyamine depleted CHO cells nor the C55.7 cells excreted any polyamines into the culture media.
authors.affiliation: Department of Biochemistry, University of Kuopio, Finland.
authors.forename: T
authors.initials: T
authors.lastname: Hyvönen
mesh_terms: D000818:Animals; D015317:Biogenic Polyamines / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D000518:Eflornithine / Q000494:pharmacology; D011700:Putrescine / Q000378:metabolism / Q000494:pharmacology; D013095:Spermidine / Q000031:analogs & derivatives / Q000378:metabolism; D013096:Spermine / Q000378:metabolism
chemical_list: D015317:Biogenic Polyamines; C030223:N(8)-acetylspermidine; C017988:N(1)-acetylspermidine; D013096:Spermine; D013095:Spermidine; D011700:Putrescine; D000518:Eflornithine