[Determination of antithrombocyte antibodies in the blood serum of patients with idiopathic thrombocytopenic purpura by an immunoenzyme method].
Enzyme-linked immunosorbent assay (ELISA) was developed for determination of serum antiplatelet antibodies. Platelets obtained from healthy donors of blood group 0(1) were washed off plasma and sedimented on the bottom of microtest wells. After washing off unattached platelets and blocking of plastic with albumin platelets were incubated with sera under investigation and binding of serum antibodies was detected using antihuman immunoglobulin antibodies conjugated with peroxidase. Ten patients with idiopathic thrombocytopenic purpura (ITP). 1 patient with systemic lupus erythematosus. 1 patient with red blood cell aplasia and 9 healthy donors (negative control) were studied by ELISA. Serum antibodies which effectively bound to platelets were detected in 5 patients with ITP, in patient with lupus erythematosus and in patient with red blood cell aplasia.
Koval'
A A
AA
Mazurov
A V
AV
Vinogradov
D V
DV
Rudin
A V
AV
Repin
V S
VS
D000017:ABO Blood-Group System / Q000276:immunology; D000906:Antibodies / Q000032:analysis*; D001792:Blood Platelets / Q000276:immunology*; D001803:Blood Transfusion; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D011696:Purpura, Thrombocytopenic / Q000276:immunology* / Q000628:therapy; D012010:Red-Cell Aplasia, Pure / Q000276:immunology / Q000628:therapy
D000017:ABO Blood-Group System; D000906:Antibodies
Opredelenie antitrombotsitarnykh antitel v syvorotke krovi bol'nykh s idiopaticheskoĭ trombotsitopenicheskoĭ purpuroĭ immunofermentnym metodom.