Botulinum C2 toxin treatment increases the G-actin pool in intact chicken cells: a model for the cytopathic action of actin-ADP-ribosylating toxins.
Botulinum C2 toxin ADP-ribosylates actin in intact chicken embryo cells in a concentration-dependent manner. This effect correlates with an enhancement in the inhibitory potency of the respective cell lysates on DNAse I activity, indicating an increase in the cellular G-actin content of toxin-treated cells. The data support our view, that ADP-ribosylation of cellular actin with subsequent depolymerization of cytoskeleton-associated F-actin to monomeric G-actin is involved in the cytotoxic effects of botulinum C2 toxin. A model of the cytopathic action of actin-ADP-ribosylating toxins is presented.
Aktories
K
K
Rudolf-Buchheim-Institut für Pharmakologie, Universität Giessen, F.R.G.
Reuner
K H
KH
Presek
P
P
Bärmann
M
M
D000199:Actins / Q000378:metabolism*; D000244:Adenosine Diphosphate / Q000378:metabolism*; D000818:Animals; D001905:Botulinum Toxins / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D003850:Deoxyribonuclease I / Q000378:metabolism; D012266:Ribose / Q000378:metabolism
D000199:Actins; D000244:Adenosine Diphosphate; D012266:Ribose; D003850:Deoxyribonuclease I; D001905:Botulinum Toxins