[In vivo study of human tumor cell proliferation, by histo-immunodetection of 5-iododeoxyuridine incorporated into DNA].
5 Iododeoxyuridine (IDU) was administered, (2.4 g by continuous infusion for 24 hrs.) without adverse effect, to 18 patients bearing thoracic tumours. 5 microns tissue sections were incubated with a monoclonal anti-IDU antibody. Cells that had incorporated IDU were identified immunohistochemically. The labelled cells number and situation vary from one tumor cell type to an other, from patient to patient and from territory to territory. The stroma components cells (inflammatory reaction, fibrosis...) were seldom labelled except for neovascularization. Normal tissues have different labelling indexes with their origin.
Duprez
A
A
Laboratoire d' Anatomie pathologique, Faculté A de Médecine, Vandaeuvre-lès-Nancy.
Borrelly
J
J
Grosdidier
G
G
Hoffmann
M
M
Boileau
S
S
D000328:Adult; D000368:Aged; D002455:Cell Division; D004247:DNA / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D007065:Idoxuridine; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D013899:Thoracic Neoplasms / Q000378:metabolism / Q000473:pathology*
D004247:DNA; D007065:Idoxuridine
Etude in vivo de la prolifération cellulaire de tumeurs humaines, par histo-immunodétection de la 5 iododésoxyuridine incorporée dans l'ADN.