title: Development changes of growth factors for parasympathetic neurons secreted by their target cells in vitro.
abstract: Trophic activities in conditioned media (CM) on target cells were quantified for their ability to maintain survival and promote neurite outgrowth of dissociated ciliary ganglia (CG) neurons from 8 day-old chick embryos in culture. CM on target cells were obtained at different stages of development. The ability of CMs to maintain neuronal survival increased almost 4-fold between 5 and 9 embryonic days, and then it was kept constant. On the other hand, neurite promoting activity was similar in all ages except in 8 days old CM that was doubled.
authors.affiliation: Departamento de Ciencias Morfológicas, Facultad de Veterinaria, Universidad de Córdoba, Spain.
authors.forename: F J
authors.initials: FJ
authors.lastname: Alcain
mesh_terms: D000818:Animals; D002642:Chick Embryo; D005123:Eye / Q000378:metabolism; D005724:Ganglia / Q000378:metabolism; D009414:Nerve Growth Factors / Q000378:metabolism*; D009474:Neurons / Q000502:physiology*; D010275:Parasympathetic Nervous System / Q000166:cytology / Q000254:growth & development / Q000502:physiology*
chemical_list: D009414:Nerve Growth Factors