title: Monoclonal antibody direct immunofluorescence for the identification of Neisseria gonorrhoeae strains grown on selective culture media.
abstract: Commercially available reagents for monoclonal antibody immunofluorescence, polyclonal antibody immunofluorescence, and coagglutination were employed in comparison with a rapid sugar fermentation test used for identifying clinical isolates of Neisseria gonorrhoeae recovered on modified Thayer-Martin media. Of 68 gonococcal strains, 68 were positive by the monoclonal immunofluorescent antibody. Of 16 nongonococcal gram-negative, oxidase-positive diplococci, all were negative by this reagent. Monoclonal antibody immunofluorescence was more specific and more sensitive than polyclonal antibody immunofluorescence and more sensitive than coagglutination.
authors.affiliation: 1st Clinic of Dermatology, University of Milan, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Cavicchini
authors.forename: E
authors.initials: E
authors.lastname: Alessi
mesh_terms: D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001431:Bacteriological Techniques; D005455:Fluorescent Antibody Technique; D006069:Gonorrhea / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D001936:Moraxella catarrhalis / Q000302:isolation & purification; D009344:Neisseria gonorrhoeae / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000911:Antibodies, Monoclonal