title: Regulatory role for GTP-binding proteins in endocytosis.
abstract: Guanosine 5'-triphosphate (GTP)-binding proteins have been implicated in the transport of newly synthesized proteins along the secretory pathway of yeast and mammalian cells. Early vesicle fusion events that follow receptor-mediated endocytosis as measured by three in vitro assays were blocked by guanosine 5'-O-(3-thiotriphosphate) and aluminum fluoride. The effect was specific for guanosine nucleotides and depended on the presence of cytosolic factors. Thus, GTP-binding proteins may also have a role in the transport of molecules along the endocytic pathway.
authors.affiliation: Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO 63110.
authors.forename: L S
authors.initials: LS
authors.lastname: Mayorga
authors.forename: R
authors.initials: R
authors.lastname: Diaz
authors.forename: P D
authors.initials: PD
authors.lastname: Stahl
mesh_terms: D001692:Biological Transport / Q000187:drug effects; D002460:Cell Line; D002474:Cell-Free System; D003600:Cytosol / Q000502:physiology; D004140:Dinitrophenols / Q000276:immunology / Q000378:metabolism; D004705:Endocytosis / Q000187:drug effects; D005089:Exocytosis; D019204:GTP-Binding Proteins / Q000502:physiology*; D005966:Glucuronidase / Q000378:metabolism; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D007074:Immunoglobulin G / Q000378:metabolism; D007163:Immunosorbent Techniques; D007425:Intracellular Membranes / Q000502:physiology; D008264:Macrophages / Q000378:metabolism / Q000648:ultrastructure; D008561:Membrane Fusion / Q000187:drug effects; D015388:Organelles / Q000648:ultrastructure; D013873:Thionucleotides / Q000494:pharmacology
chemical_list: D004140:Dinitrophenols; D007074:Immunoglobulin G; D013873:Thionucleotides; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate; D005966:Glucuronidase; D019204:GTP-Binding Proteins