Effect of etoposide (VP16-213) on lipid peroxidation and antioxidant status in a high-dose radiochemotherapy regimen.
A total of 13 patients receiving bone marrow transplants (BMT) for treatment of different haematological diseases were investigated. Conditioning therapy preceding BMT consisted of fractionated total-body irradiation (12 Gy) and high-dose chemotherapy with cyclophosphamide (2 +/- 60 mg/kg). Patients stratified to be at high risk for relapse (6/13) were additionally treated with etoposide (30 mg/kg). Plasma concentrations of absolute and lipid-standardized antioxidants (alpha-tocopherol and beta-carotene) decreased following conditioning therapy, presumably as the result of an enhanced breakdown of these antioxidants. Etoposide treatment did not amplify the loss of essential anti-oxidants but significantly increased lipid hydroperoxide concentrations in serum. We suggest that the abnormal generation of lipid hydroperoxides is the result of free radical formation.
Ladner
C
C
Eberhard-Karls-Universität Tübingen, Medizinische Klinik und Poliklinik, Federal Republic of Germany.
Ehninger
G
G
Gey
K F
KF
Clemens
M R
MR
D000741:Anemia, Aplastic / Q000097:blood / Q000628:therapy; D000975:Antioxidants; D016026:Bone Marrow Transplantation / Q000379:methods; D002338:Carotenoids / Q000097:blood; D003131:Combined Modality Therapy; D005047:Etoposide / Q000008:administration & dosage*; D006801:Humans; D007938:Leukemia / Q000097:blood / Q000628:therapy; D015227:Lipid Peroxidation / Q000187:drug effects*; D008054:Lipid Peroxides / Q000097:blood; D009190:Myelodysplastic Syndromes / Q000097:blood / Q000628:therapy; D010288:Parenteral Nutrition; D011879:Radiotherapy Dosage; D013997:Time Factors; D014810:Vitamin E / Q000097:blood; D019207:beta Carotene
D000975:Antioxidants; D008054:Lipid Peroxides; D019207:beta Carotene; D014810:Vitamin E; D002338:Carotenoids; D005047:Etoposide