Genetic comparison of bacteriophage PS17 and Pseudomonas aeruginosa R-type pyocin.
PS17 is a bacteriophage of Pseudomonas aeruginosa that is serologically cross-reactive with phage tail-like bacteriocins called R-type pyocins. In addition to having immunological cross-reactivity, certain genes are functionally complementable between PS17 and R-type pyocins. To compare the genetic structures of PS17 and R-type pyocins, a physical map of PS17 genes was constructed by cloning phage DNA fragments on RSF1010-derived vector plasmids. The head and tail gene clusters were tandemly arrayed and together occupied about half of the 41-kilobase-pair PS17 chromosome. With use of these phage clones, the following results were obtained with respect to the genetic relationship between PS17 and R-type pyocins: (i) serological cross-reaction between PS17 and pyocin occurred for the major sheath protein and two components of the fiber, (ii) a certain pyocin mutation was complemented by cloned phage fragments, and (iii) the phage DNA fragment carrying sheath and core tube genes was shown to hybridize to the DNA fragment carrying the pyocin R2 genes.
Shinomiya
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T
Mitsubishi-Kasei Institute of Life Sciences, Tokyo, Japan.
Ina
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S
D001430:Bacteriocins / Q000235:genetics*; D001435:Bacteriophages / Q000235:genetics*; D015153:Blotting, Western; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D005798:Genes, Bacterial; D005814:Genes, Viral; D005816:Genetic Complementation Test; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D011709:Pyocins / Q000235:genetics* / Q000276:immunology; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000235:genetics
D001430:Bacteriocins; D004279:DNA, Viral; D011709:Pyocins; D014764:Viral Proteins