title: Differential expression of a gene highly homologous to c-ras during the development of the brine shrimp Artemia.
abstract: We report the identification of p21ras and a cDNA coding for it in the crustacean Artemia. The monoclonal antibody Y13-259 immunoprecipitates a polypeptide of 21.5 kDa in 24 hr-old larvae. The homology of p21ras with the Drosophila melanogaster and the mammalian p21s is in the order of 75-80% mRNA (of 1.2 kb in length) is already present in encysted gastrulae, and the levels increase, reaching a maximum before emergence. On the contrary, both the amount of p21ras and its GTP-binding activity are low prior to emergence and rises afterwards. These results suggest that p21ras expression is regulated post-transcriptionally, and that its function(s) is needed for post-hatching events, the more likely being the resumption of cell proliferation.
authors.affiliation: Instituto de Investigaciones Biomédicas del CSIC, Madrid, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Díaz-Guerra
authors.forename: M
authors.initials: M
authors.lastname: Quintanilla
authors.forename: I
authors.initials: I
authors.lastname: Palmero
authors.forename: L
authors.initials: L
authors.lastname: Sastre
authors.forename: J
authors.initials: J
authors.lastname: Renart
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001156:Artemia / Q000235:genetics / Q000254:growth & development*; D001483:Base Sequence; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004331:Drosophila melanogaster / Q000235:genetics; D005786:Gene Expression Regulation; D007163:Immunosorbent Techniques; D007814:Larva / Q000378:metabolism; D008969:Molecular Sequence Data; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016283:Proto-Oncogene Proteins p21(ras); D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000096:biosynthesis; D012689:Sequence Homology, Nucleic Acid
chemical_list: D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D004247:DNA; D016283:Proto-Oncogene Proteins p21(ras)