Effect of bovine follicular fluid and follicle-stimulating hormone on follicular growth in unilaterally ovariectomized prepuberal heifers.
A study was conducted to determine if charcoal-extracted follicular fluid inhibits FSH-induced follicular development in prepuberal heifers. Thirty-six prepuberal heifers were allotted by breed and weight to a 2 x 2 factorial experiment involving charcoal-extracted follicular fluid and FSH treatments. Heifers were unilaterally ovariectomized and injected (intravenously; 10 ml) every 8 h for 88 h with either charcoal-extracted follicular fluid or saline. Follicle-stimulating hormone (2 mg) or saline was injected (intramuscularly) every 8-h starting 24 h after initiation of charcoal-extracted follicular fluid to 88 h following unilateral ovariectomy. Plasma samples were collected at 8-h intervals from 48 h prior to unilateral ovariectomy to 96 h following unilateral ovariectomy when the remaining ovary was removed. Follicular fluid and total ovarian weight increased following FSH treatment. The increases were not inhibited by charcoal-extracted follicular fluid. Total number of surface follicles was similar among treatments. However, FSH induced a shift in follicular diameter from small (less than or equal to 3 mm) to medium (7 to 9 mm) or large (10 to 13 mm) follicles, which was unaffected by charcoal-extracted follicular fluid. Plasma concentration of FSH, but not LH, declined following charcoal-extracted follicular fluid administration. In summary, charcoal-extracted follicular fluid did not inhibit FSH-induced follicular development in prepuberal heifers when charcoal-extracted follicular fluid was administered at a dosage that reduced circulating concentration of FSH by approximately 40%.
Moser
M T
MT
Dairy Science Department, University of Missouri, Columbia 65211.
Garverick
H A
HA
Smith
M F
MF
Youngquist
R S
RS
D000818:Animals; D002417:Cattle / Q000502:physiology*; D004958:Estradiol / Q000378:metabolism; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood / Q000494:pharmacology*; D015571:Follicular Fluid / Q000378:metabolism / Q000502:physiology*; D006984:Hypertrophy / Q000503:physiopathology; D016014:Linear Models; D007986:Luteinizing Hormone / Q000097:blood; D006080:Ovarian Follicle / Q000254:growth & development*; D010052:Ovariectomy / Q000662:veterinary*; D010053:Ovary / Q000473:pathology; D011374:Progesterone / Q000378:metabolism; D013739:Testosterone / Q000378:metabolism
D013739:Testosterone; D011374:Progesterone; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone