HLA class II typing using oligonucleotide probes.
A DQ beta locus-specific oligonucleotide probe is used to identify DQ beta alleles present in six insulin-dependent diabetes mellitus (IDDM) multiplex families. Results of genotyping using this technique agree with those from cDNA probes and are often clearer. Allele-specific oligonucleotide probes for the DR beta locus are used to identify subtypes (Dw4 and Dw14) of the DR4 specificity in five IDDM multiplex families. This distinction cannot be made with available cDNA probes.
Robinson
D M
DM
Virginia Mason Research Center, Seattle, WA 98101.
Holbeck
S
S
Seyfried
C
C
Byers
P
P
Palmer
J
J
Nepom
G T
GT
D015139:Blotting, Southern; D003625:Data Collection; D003922:Diabetes Mellitus, Type 1 / Q000453:epidemiology / Q000235:genetics*; D005802:Genes, MHC Class II; D006681:HLA-D Antigens / Q000235:genetics; D006684:HLA-DR Antigens / Q000235:genetics; D015804:HLA-DR4 Antigen; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D006801:Humans; D015345:Oligonucleotide Probes; D015183:Restriction Mapping
D006681:HLA-D Antigens; D006684:HLA-DR Antigens; D015804:HLA-DR4 Antigen; C050381:HLA-Dw14 antigen; C053495:HLA-Dw4 antigen; D000949:Histocompatibility Antigens Class II; D015345:Oligonucleotide Probes