[Pro-cathepsin D can activate in vitro pro-cathepsin B secreted by ovarian cancers].
A precursor form of cathepsin B (Mr 45-47 kd) was purified from ascitic fluids of patients with ovarian adenocarcinomas. Following pepsin activation, this precursor produced a 33 kd cathepsin B-like proteinase closely related to lysosomal cathepsin B. A similar activation was found using the 52 kd pro-cathepsin D secreted by the MCF7 human breast cancer cells. This activation was a time, dose and pH dependent process. These results suggest that the 52 kd pro-cathepsin D may be involved in the early steps of the "metastatic cascade", activating pro-cathepsin B in an acidic environment.
Pagano
M
M
Laboratoire de Biochimie, U.E.R. Broussais-Hôtel-Dieu, Paris.
Capony
F
F
Rochefort
H
H
D000230:Adenocarcinoma / Q000378:metabolism*; D002401:Cathepsin B / Q000378:metabolism*; D002402:Cathepsin D / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004792:Enzyme Precursors / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology; D005260:Female; D006801:Humans; D008970:Molecular Weight; D010051:Ovarian Neoplasms / Q000378:metabolism*; D013997:Time Factors
D004792:Enzyme Precursors; D002401:Cathepsin B; D002402:Cathepsin D
La pro-cathepsine D peut activer in vitro la pro-cathepsine B sécrétée par les cancers ovariens.