A comparison of silica and alumina columns for high-performance liquid chromatographic separations of basic drugs and plasma constituents following on-line solid-phase extraction with column switching.
A comparison has been made of a silica and an alumina column with respect to retention behaviour in high-performance liquid chromatography of pure drug solutions and plasma profiles following on-line solid-phase extraction. Using methanol-ammonium nitrate buffer (80:20, v/v) as eluent, it was found that these systems lend themselves well to the column switching technique. Increasing the pH of the mobile phase produced similar shifts in retention for basic drugs on both columns, but fewer plasma interferents are retained on the alumina column as compared to the silica column under the same operating conditions.
Kelly
M T
MT
School of Chemical Sciences, Dublin City University, Glasnevin, Ireland.
Smyth
M R
MR
D000537:Aluminum Oxide; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D004364:Pharmaceutical Preparations / Q000032:analysis*; D012015:Reference Standards; D012997:Solvents
D007202:Indicators and Reagents; D004364:Pharmaceutical Preparations; D012997:Solvents; D000537:Aluminum Oxide