title: Determination of the absolute concentrations of monoamine oxidase A and B in human tissues.
abstract: The concentrations of monoamine oxidase-A and -B were determined in homogenates of human cerebral cortex, caudatus and placenta and in human platelet-rich plasma and platelet membranes by determining the specific binding of tritium-labelled pargyline. The concentrations of the two enzyme forms were similar in both human brain regions examined. Determinations of the minimum quantities of clorgyline, (-)-deprenyl, J-508 or pargyline necessary to give complete inhibition of enzyme activity was found to give overestimates of the amounts of monoamine oxidase-A and -B present due to nonspecific binding of these inhibitors.
authors.affiliation: Department of Biochemistry, Trinity College, Dublin, Ireland.
authors.forename: A M
authors.initials: AM
authors.lastname: O'Carroll
authors.forename: M C
authors.initials: MC
authors.lastname: Anderson
authors.forename: I
authors.initials: I
authors.lastname: Tobbia
authors.forename: J P
authors.initials: JP
authors.lastname: Phillips
authors.forename: K F
authors.initials: KF
authors.lastname: Tipton
mesh_terms: D001344:Autopsy; D001665:Binding Sites / Q000187:drug effects; D001792:Blood Platelets / Q000201:enzymology*; D001921:Brain / Q000201:enzymology*; D003010:Clorgyline / Q000494:pharmacology; D006801:Humans; D008995:Monoamine Oxidase / Q000032:analysis*; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology; D010293:Pargyline / Q000494:pharmacology; D010920:Placenta / Q000201:enzymology*; D012642:Selegiline / Q000494:pharmacology
chemical_list: D008996:Monoamine Oxidase Inhibitors; D012642:Selegiline; D010293:Pargyline; D008995:Monoamine Oxidase; D003010:Clorgyline