[Erythrocyte diagnostic kits for detection of Pseudomonas aeruginosa exotoxin A].
The use of formulated chick red blood cells loaded with IgG preparations and affinity-purified antibodies, in comparison with initial immune serum to P. aeruginosa exotoxin A (ETA), has been shown to increase the sensitivity of antibody erythrocyte diagnosticum (AbED) 17-fold and to ensure the detection of ETA at a concentration of 1.2 mg of protein per ml. The passive hemagglutination (PHA) test with AbED has proved to be a more sensitive method for the detection of ETA than the antibody neutralization test with the use of antigenic erythrocyte diagnosticum, the latex agglutination test, the coagglutination test and the enzyme immunoassay. The PHA test has permitted the detection of ETA in the culture fluid of 80% of P. aeruginosa cultures under study.
Deriabin
P N
PN
Aleksandrova
I A
IA
Karal'nik
B V
BV
Moroz
A F
AF
Aleksandrov
A D
AD
D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D003470:Culture Media; D004912:Erythrocytes; D005098:Exotoxins / Q000032:analysis*; D006801:Humans; D007106:Immune Sera; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D003470:Culture Media; D005098:Exotoxins; D007106:Immune Sera; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
Eritrotsitarnye diagnostikumy dlia vyiavleniia ékzotoksina A Pseudomonas aeruginosa.