[Development of molecular probes for the diagnosis of mycobacteria].
The presence of a repetitive sequence in Mycobacterium bovis DNA was demonstrated. This sequence was also found in M. tuberculosis DNA but was absent in M. kansasii, M. flavescens, M. fortuitum, M. vaccae, M. leprae, M. phlei, M. smegmatis and M. marinum. This repetitive sequence was found to be polymorphic in M. tuberculosis but not in BCG. Dot hybridization analysis suggested that the repetitive sequence could be useful in the identification of pathogenic Mycobacterium in clinical samples.
Franco
R
R
Hospital Nacional Baldomero Sommer, General Rodríguez, Provincia de Buenos Aires, Argentina.
Ruiz Trevisan
A
A
Zorzopulos
J
J
D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics*; D009161:Mycobacterium / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*
D015342:DNA Probes; D004269:DNA, Bacterial
Construcción de sondas moleculares para el diagnóstico de micobacterias.