Precipitating antibodies of the duck (Anas platyrhynchos).
1. Ducks do not usually produce precipitating antibodies. However, when hyperimmunized with the dinitrophenyl (DNP)-human IgG (HIgG) conjugate, ducks made precipitating antibodies to the HIgG carrier, though not to the DNP hapten. 2. Precipitation did not require NaCl and occurred over a wide range of molarities and pH. 3. Antigenic and polyacrylamide gel analysis of affinity-purified antibodies, indicated that the major constituent of the antibody population was electrophoretically homogeneous 5.7S IgG. 4. The Ig heavy chains were in two populations, viz MW 37,000 and 41,000; non-precipitating duck 5.7S IgG antibodies to HIgG had only the MW 37,000 heavy chains. 5. It is suggested that duck precipitins belong to a previously unrecognized subclass of duck 5.7S IgG, with minor physical and antigenic differences from their non-precipitating counterparts.
Higgins
D A
DA
Department of Pathology, University of Hong Kong, Queen Mary Hospital.
D000818:Animals; D000917:Antibody Formation; D011232:Chemical Precipitation; D004136:Dinitrobenzenes / Q000276:immunology; D004372:Ducks / Q000276:immunology*; D006241:Haptens; D007074:Immunoglobulin G / Q000096:biosynthesis; D007143:Immunoglobulin Heavy Chains / Q000302:isolation & purification; D008970:Molecular Weight
D004136:Dinitrobenzenes; D006241:Haptens; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains