[The detection of Bacillus anthracis protective antigens by enzyme immunoassay (EIA) using polyclonal and monoclonal antibodies].
An Enzyme-Immunoassay (EIA) for the detection of Bacillus anthracis-protective antigen (PA) within one hour was developed. If the rabbit antiserum was used, 15 ng PA/ml could be detected and with the monoclonal antibody, the detection limit was 60 ng PA/ml. With respect to the higher specificity and with regard to the aspects of animalcare, monoclonal antibodies should be used in the test instead of the polyclonal antiserum.
Kleine-Albers
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Böhm
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D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis*; D001408:Bacillus anthracis / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology; D007124:Immunoenzyme Techniques; D051379:Mice; D011237:Predictive Value of Tests; D011817:Rabbits
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D007106:Immune Sera
Nachweis von Bacillus anthracis-Schutzantigen mittels Enzym-Immunoassay (EIA) unter Verwendung von polyklonalen sowie monoklonalen Antikörpern.