Obligatory role of macrophages in dengue virus antigen presentation to B lymphocytes.
The study was undertaken to investigate the role of dengue type 2 virus (DV)-infected mouse peritoneal macrophages (M phi) in presentation of the DV antigen to B lymphocytes as shown by counting virus-specific IgM antibody plaque-forming cells (PFC). It was observed that heat-killed or glutaraldehyde-fixed M phi did not present the antigen. Pretreatment of M phi with the lysosomotropic compounds ammonium chloride and chloroquine inhibited the antigen presentation. Depletion of M phi from the spleen cell cultures abrogated the immune response to DV. The tryptic-digested DV antigen could stimulate immune responses in B-lymphocyte enriched (depleted of M phi and T cells) spleen cell cultures, and the digested antigen could be presented by glutaraldehyde-fixed M phi. Pretreatment of M phi with a trypsin inhibitor abrogated antigen presentation. The findings thus show that even for presentation to B cells the DV antigen must be processed by M phi by a trypsin-like protease.
Rizvi
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Postgraduate Department of Microbiology, K.G. Medical College, Lucknow, India.
Chaturvedi
U C
UC
Mathur
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A
D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D000938:Antigen-Presenting Cells / Q000187:drug effects / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D002738:Chloroquine / Q000494:pharmacology; D003716:Dengue Virus / Q000276:immunology*; D005976:Glutaral / Q000494:pharmacology; D006358:Hot Temperature; D008213:Lymphocyte Activation; D008264:Macrophages / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D014360:Trypsin Inhibitor, Kunitz Soybean / Q000494:pharmacology
D000956:Antigens, Viral; D000643:Ammonium Chloride; D002738:Chloroquine; D014360:Trypsin Inhibitor, Kunitz Soybean; D005976:Glutaral