Effect of a mammary-derived growth inhibitor on the expression of the oncogenes c-fos, c-myc and c-ras.
A mammary-derived growth inhibitor (MDGI) inhibits the resumption of growth of stationary Ehrlich ascites carcinoma (EAC) cells in vitro. The present study shows that the resumption of growth is accompanied by a rapid increase of the steady state mRNA level of the proto-oncogenes c-fos, c-myc and c-ras, which is reduced by MDGI. EAC cells from the exponential growth phase insensitive to MDGI did not show a reduced RNA expression. The effect of MDGI represents a novel activity at the level of gene expression and suggests a link to exist between growth inhibition and the reduction of c-fos, c-myc and c-ras expression.
Lehmann
W
W
Central Institute for Molecular Biology, Academy of Sciences, Berlin-Buch, GDR.
Strauss
M
M
Kiessling
U
U
Graetz
H
H
Koberling
A
A
Langen
P
P
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism / Q000473:pathology; D002417:Cattle; D002455:Cell Division; D005786:Gene Expression Regulation; D006131:Growth Inhibitors / Q000494:pharmacology*; D007700:Kinetics; D008321:Mammary Glands, Animal / Q000032:analysis*; D051379:Mice; D011518:Proto-Oncogene Proteins / Q000235:genetics; D016760:Proto-Oncogene Proteins c-fos; D016271:Proto-Oncogene Proteins c-myc; D016283:Proto-Oncogene Proteins p21(ras); D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000378:metabolism; D014407:Tumor Cells, Cultured
D006131:Growth Inhibitors; D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D016283:Proto-Oncogene Proteins p21(ras)