title: Interferon-gamma treatment increases HLA-DR expression on monocytes in severely injured patients.
abstract: Traumatic injury and sepsis reduce HLA-DR expression on monocytes. This study assessed the effect of interferon-gamma (IFN-gamma) on the expression of HLA-DR on monocytes taken from severely injured patients. The mononuclear layer of each blood sample was incubated with either 500 units IFN-gamma or control media. The mean baseline HLA-DR expression of the trauma group (n = 11) was 32%, significantly less than the mean HLA-DR expression of 91% for the control group (n = 10). In the trauma group, HLA-DR expression after culturing with IFN-gamma was 81%, significantly greater than both the mean baseline HLA-DR expression (32%). In the control group, HLA-DR expression after culturing with IFN-gamma was 94%, similar to the mean baseline HLA-DR expression of 91%. These data confirm that HLA-DR on monocytes of severely injured patients is markedly reduced and show that it can be increased significantly in vitro by IFN-gamma.
authors.affiliation: Institute of Surgical Research, Department of Surgery, School of Medicine, University of Louisville, KY.
authors.forename: M J
authors.initials: MJ
authors.lastname: Hershman
authors.forename: S H
authors.initials: SH
authors.lastname: Appel
authors.forename: S R
authors.initials: SR
authors.lastname: Wellhausen
authors.forename: G
authors.initials: G
authors.lastname: Sonnenfeld
authors.forename: H C
authors.initials: HC
authors.lastname: Polk
mesh_terms: D002478:Cells, Cultured; D006684:HLA-DR Antigens / Q000032:analysis*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D009000:Monocytes / Q000187:drug effects* / Q000276:immunology; D014947:Wounds and Injuries / Q000276:immunology*
chemical_list: D006684:HLA-DR Antigens; D007371:Interferon-gamma