title: Introduction of macromolecules into primary cultured neuronal cells by fusion with erythrocyte ghosts.
abstract: The macromolecules were injected into primary cultured neuronal cells by fusion with human erythrocyte ghosts containing macromolecules. The injection efficiency was about 30% and 80% of the cells extended neurites like normal neuronal cells. In order to succeed in this method, phytohaemagglutinin E and polyethylene glycol 1000 were essential, and monolayer culture cells were used.
authors.affiliation: Department of Physiology, Yokohama City University School of Medicine, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Hikawa
authors.forename: H
authors.initials: H
authors.lastname: Horie
authors.forename: T
authors.initials: T
authors.lastname: Kawakami
authors.forename: K
authors.initials: K
authors.lastname: Okuda
authors.forename: T
authors.initials: T
authors.lastname: Takenaka
mesh_terms: D002459:Cell Fusion / Q000187:drug effects; D002478:Cells, Cultured; D004910:Erythrocyte Membrane / Q000378:metabolism*; D005727:Ganglia, Spinal / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000378:metabolism*; D007142:Immunoglobulin gamma-Chains / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D010835:Phytohemagglutinins; D011092:Polyethylene Glycols; D012710:Serum Albumin, Bovine / Q000378:metabolism*
chemical_list: D007143:Immunoglobulin Heavy Chains; D007142:Immunoglobulin gamma-Chains; D010835:Phytohemagglutinins; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols