title: Bleeding diathesis due to decreased functional activity of type 1 plasminogen activator inhibitor.
abstract: We evaluated an elderly patient with a lifelong history of severe bleeding after surgery or trauma and with evidence of persistent hyperfibrinolysis. Routine coagulation studies were normal. Serum plasminogen (40%, normal 72-128%) and alpha 2-antiplasmin (55%, normal 70-145%) activities were decreased. Euglobulin clot lysis was abnormally shortened (50 min) and normalized in vitro with epsilon-aminocaproic acid (EACA). The patient was treated with EACA with prompt cessation of bleeding. Patient tissue-plasminogen activator (t-PA) levels in serum were normal (4.7 ng/ml, control 3.5-7.2) as detected by a two-site immunoradiometric assay (IRMA). Patient fibrinolytic inhibitor activities were assessed by incubating 125I-labeled t-PA with either whole blood or serum followed by SDS-PAGE and autoradiography to identify the resultant protease/protease inhibitor complexes. In comparison to blood samples obtained from normal donors, patient plasma and serum demonstrated reduced binding of a fast-acting plasminogen activator inhibitor to 125I-labeled t-PA. Immunoprecipitation experiments indicated diminished complex formation between type 1 plasminogen activator inhibitor (PAI-1) in patient serum and 125I-labeled t-PA. Low patient PAI-1 activity was confirmed in serum (0.36 U/ml, control 0.87-1.81; n = 3) and in platelet lysates using a functional IRMA to quantitate PAI-1 binding to immobilized t-PA. However, patient serum PAI-1 antigen was within the normal range when analyzed by IRMA (31.8 ng/ml, control 19.6-42.2); this result was confirmed in both serum and platelets by Western blot (n = 3). Mixing experiments using purified PAI-1 as well as patient and control sera did not show evidence for an inhibitor against PAI-1. We conclude that this patient's bleeding diathesis was due to hyperfibrinolysis and defective PAI-1. This patient provides the first demonstration of a link between decreased in vivo PAI-1 activity and disordered hemostasis, and supports a role for PAI-1 in control of vivo fibrinolysis.
authors.affiliation: Department of Medicine, Stanford University Medical Center, California 94305.
authors.forename: R R
authors.initials: RR
authors.lastname: Schleef
authors.forename: D L
authors.initials: DL
authors.lastname: Higgins
authors.forename: E
authors.initials: E
authors.lastname: Pillemer
authors.forename: L J
authors.initials: LJ
authors.lastname: Levitt
mesh_terms: D000368:Aged; D015153:Blotting, Western; D005342:Fibrinolysis; D006023:Glycoproteins / Q000097:blood / Q000276:immunology / Q000494:pharmacology*; D006474:Hemorrhagic Disorders / Q000097:blood / Q000209:etiology*; D006801:Humans; D007518:Isoantibodies / Q000502:physiology; D008297:Male; D010447:Peptide Hydrolases; D015849:Plasminogen Inactivators; D011233:Precipitin Tests; D010959:Tissue Plasminogen Activator / Q000037:antagonists & inhibitors*
chemical_list: D006023:Glycoproteins; D007518:Isoantibodies; D015849:Plasminogen Inactivators; D010447:Peptide Hydrolases; D010959:Tissue Plasminogen Activator