Production of prostaglandins by fetal rat lung type II pneumonocytes and fibroblasts.
The output of prostaglandins I2, E2, F2 alpha and 13,14-dihydro-15-keto-PGF2 alpha (PGFM) from third passage day 20 rat fetal fibroblasts and type II alveolar pneumonocytes was studied. In 2 h incubations, the output levels for each cell type were: PGI2 greater than PGE2 much greater than PGF2 alpha = PGFM when cells were incubated with Ca2+ ionophore A23187 (10 microM) or arachidonic acid (1 microgram/ml).
Olson
D M
DM
Department of Paediatrics, St. Joseph's Health Centre of London, University of Western Ontario, Canada.
Tanswell
A K
AK
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002478:Cells, Cultured; D015232:Dinoprostone / Q000096:biosynthesis; D011464:Epoprostenol / Q000096:biosynthesis; D005347:Fibroblasts / Q000378:metabolism; D066298:In Vitro Techniques; D008168:Lung / Q000196:embryology / Q000378:metabolism*; D011453:Prostaglandins / Q000096:biosynthesis*; D051381:Rats
D001095:Arachidonic Acids; D011453:Prostaglandins; D016718:Arachidonic Acid; D000001:Calcimycin; D011464:Epoprostenol; D015232:Dinoprostone