Evaluation of a phenolglycolipid antigen (PGL-Tb 1) from M. tuberculosis in the serodiagnosis of tuberculosis: comparison with PPD antigen.
Sera from 38 tuberculous patients and 62 healthy controls (31 PPD skin test positive and 31 negative) were assayed, by enzyme-linked immunosorbent assay (ELISA), to test the activity of IgG and IgM antibodies against purified protein derivative (PPD) antigen and a phenolglycolipid antigen (PLG-Tb 1) isolated and purified from Mycobacterium tuberculosis strain Canetti. Using PPD antigen, the sensitivity and specificity were respectively, 50 and 93.5% for IgG and 71.1 and 59.7% for IgM antibody activity. Against PGL-Tb 1 antigen, IgG had a sensitivity of 94.7% and the specificity was 96.8%, for IgM antibody they were 65.8% and 75.8% respectively. The ELISA using PGL-Tb 1 antigen could be a useful way to develop a rapid technique to aid in the diagnosis of tuberculosis.
Martín Casabona
N
N
Laboratory of Microbiology and Parasitology, Ciutat Sanitària Vall d'Hebron, Universitat Autònoma de Barcelona, Spain.
Gonzalez Fuente
T
T
Arcalis Arce
L
L
Otal Entraigas
J
J
Vidal Pla
R
R
D000907:Antibodies, Bacterial / Q000302:isolation & purification; D000942:Antigens, Bacterial; D003951:Diagnostic Errors; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D006017:Glycolipids / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000302:isolation & purification; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D012698:Serologic Tests; D014373:Tuberculin; D014376:Tuberculosis / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D006017:Glycolipids; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D014373:Tuberculin; C045169:phenolic glycolipid I, Mycobacterium leprae