[Plasma determination of 7 common drugs by high performance liquid chromatography].
A high performance liquid-chromatographic procedure is reported for measuring the plasma concentration of 5 anticonvulsants: phenobarbital, phenytoin, carbamazepine, ethosuximide, valproic acid and 2 bronchodilators: theophylline and caffeine. Limits of detection of ethosuximide and valproic acid are 28 mumol/l, 5 mumol/l for the other drugs. Linearity limits are always very upper than the higher therapeutic dose concentration, the inter assay relative standard deviation are less than 8 p. cent for the 7 drugs at the 3 levels of concentration and the analytical recoveries ranged from 93 to 104 p. cent. The technique is simple and rapid: 4 anticonvulsants are simultaneously extracted and dosed, valproic acid, only has to be dosed lonely. Finally, comparatively to the other immunoassay technique, this method is cheaper.
Richard
L
L
Laboratoire de Biochimie, Saint Germain en Laye.
Leducq
B
B
Baty
C
C
Jambou
J
J
D000927:Anticonvulsants / Q000097:blood*; D001993:Bronchodilator Agents / Q000097:blood*; D002110:Caffeine / Q000097:blood; D002220:Carbamazepine / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D005013:Ethosuximide / Q000097:blood; D010634:Phenobarbital / Q000097:blood; D010672:Phenytoin / Q000097:blood; D013806:Theophylline / Q000097:blood; D014635:Valproic Acid / Q000097:blood
D000927:Anticonvulsants; D001993:Bronchodilator Agents; D002220:Carbamazepine; D002110:Caffeine; D005013:Ethosuximide; D014635:Valproic Acid; D010672:Phenytoin; D013806:Theophylline; D010634:Phenobarbital
Dosage plasmatique de sept médicaments usuels par chromatographie liquide haute performance.