Complete nucleotide sequence of ovine beta-casein cDNA: inter-species comparison.
The complete nucleotide sequence of ovine beta-casein mRNA has been determined by sequencing, according to Sanger-Messing, both a recombinant clone isolated from a mammary cDNA pUC 18 library and a single-stranded cDNA generated by reverse transcription from a synthetic 17-mer primer complementary to the 5' part of the mRNA coding frame. The 1088 nucleotide long beta-casein mRNA, excluding the poly(A) tail, contains a coding frame of 669 nucleotides including the stop codon, flanked by 60 and 359 nucleotides in the 5' and 3' untranslated regions, respectively. It arises from the splicing of 9 exons as deduced from gene sequence data. The deduced amino acid sequence differs at 3 positions from that previously determined by direct sequencing of mature beta-casein. Comparison of the ovine, bovine, rat, mouse, and rabbit beta-casein mRNA sequences shows a higher homology in the 3' and 5' untranslated regions. The most conserved regions in the open reading frame are essentially those encoding the signal peptide and the major phosphorylation site.
Provot
C
C
Laboratoire de Génétique Biochimique, INRA-CRJ, Jouy-en-Josas, France.
Persuy
M A
MA
Mercier
J C
JC
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D002364:Caseins / Q000235:genetics*; D002417:Cattle; D004247:DNA / Q000235:genetics*; D005091:Exons; D005796:Genes; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D051381:Rats; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D012756:Sheep / Q000235:genetics*
D002364:Caseins; D012333:RNA, Messenger; D004247:DNA