[Expression of human interferon beta gene controlled by SV40 late promoter in mouse myeloma SP2/0 cells].
The HindII fragment of human interferon beta (IFN-beta) gene was inserted downstream from SV40 late promoter in pSV2-dhfr and cotransfered with pSV2-gpt into the mouse myeloma SP2/0 cells which were hypoxanthine guanine phosphoribosyl transferase (HGPRT) deficient. After the selection in HAT (Hypoxanthine Aminopterine Thymidine) medium containing myciphenolic acid and xanthine, the efficient constitutive expression of IFN-beta could be detected in the supernatant of the survive cells.
Wang
X
X
Yu
M
M
Wang
J
J
Wu
S
S
Hu
Y
Y
Hou
Y
Y
D000818:Animals; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007370:Interferon Type I / Q000235:genetics*; D051379:Mice; D009101:Multiple Myeloma / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014162:Transfection
D007370:Interferon Type I