Logarithmic timebase for stopped-flow data acquisition and analysis.
A method for capturing stopped-flow and other rapid reaction records in logarithmic time, using a microcomputer, is described. Apart from the ability to record processes over several decades in time in a single experiment, the method shows distinct advantages in subsequent nonlinear regression analysis of multiexponential processes. The method is illustrated by a study of the binding of NADPH to dihydrofolate reductase and the reaction between formycin triphosphate and heavy meromyosin.
Walmsley
A R
AR
Department of Biochemistry, University of Leicester, United Kingdom.
Bagshaw
C R
CR
D000818:Animals; D005573:Formycins / Q000378:metabolism; D007780:Lacticaseibacillus casei / Q000201:enzymology; D008432:Mathematical Computing; D008838:Microcomputers; D008974:Mollusca / Q000201:enzymology; D009132:Muscles / Q000032:analysis; D015879:Myosin Subfragments / Q000378:metabolism; D012265:Ribonucleotides / Q000378:metabolism; D013762:Tetrahydrofolate Dehydrogenase / Q000378:metabolism; D013995:Time
D005573:Formycins; D015879:Myosin Subfragments; D012265:Ribonucleotides; C032552:formycin triphosphate; D013762:Tetrahydrofolate Dehydrogenase