title: Human placental microvilli as a source of antigen for the preparation of a polyclonal antibody directed against the LDL receptor.
abstract: Polyclonal antibodies were prepared by immunization of rabbits with partially purified LDL receptor obtained from human placental microvilli. The antiserum reacted with membranes from human placental microvilli and human fibroblasts, as assessed by immunobinding studies. It also reacted with purified LDL receptors of both origins. The antiserum markedly inhibited 125I-labeled LDL binding to cultured human fibroblasts.
authors.affiliation: Laboratoire de Biochimie, Faculté de Médecine Saint-Antoine, Paris, France.
authors.forename: S
authors.initials: S
authors.lastname: Goldstein
authors.forename: M
authors.initials: M
authors.lastname: Moreau
authors.forename: V
authors.initials: V
authors.lastname: Barbu
authors.forename: M
authors.initials: M
authors.lastname: Meier
authors.forename: E
authors.initials: E
authors.lastname: Alsat
authors.forename: C
authors.initials: C
authors.lastname: Mazière
authors.forename: J C
authors.initials: JC
authors.lastname: Mazière
authors.forename: J
authors.initials: J
authors.lastname: Polonovski
mesh_terms: D000917:Antibody Formation; D015153:Blotting, Western; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D008871:Microvilli / Q000276:immunology; D010920:Placenta / Q000276:immunology* / Q000648:ultrastructure; D011973:Receptors, LDL / Q000276:immunology* / Q000378:metabolism
chemical_list: D011973:Receptors, LDL