[Immunofluorescence serologic findings in schistosomiasis. Methodologic aspects].
In 101 Africans with schistosomiasis the IFAT showed sensitivity in 71%, in which cases frozen sections of adult Mansoni-bilharzias were used as antigen. An observation of the sensitivity of the IFAT separated according to the species of parasites for the affection with S. mansoni resulted in a by 23% higher sensitivity in contrast to the S. haematobium affection. In double infestation the sensitivity was 77%. Using a soluble S. mansoni antigen the ELISA had a sensitivity of 53% in the affection by S. mansoni and of 33% in the affection by S. haematobium. One month after the treatment the sensitivity of the two methods at first increased (IFAT by 19%, ELISA by 21%) and during the further observation decreased to the initial values 6 months after the therapy and after 1 year still further diminished. In 42 Europeans free of helminths the specificity of the IFAT was 93% and in 52 Africans free of schistosomes from regions with schistosomiasis endemy 88%. The accuracy of the IFAT for the qualitative statement positive and negative was greatest in the serum dilution of 1:26.
Schlisio
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W
Abteilung für Tropenmedizin und Infektionskrankheiten, Bereiches Medizin der Wilhelm-Pieck-Universität Rostock.
Möller
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FW
Ziegler
K
K
D000293:Adolescent; D000328:Adult; D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D008875:Middle Aged; D012548:Schistosoma haematobium / Q000276:immunology*; D012550:Schistosoma mansoni / Q000276:immunology*; D012553:Schistosomiasis haematobia / Q000175:diagnosis*; D012555:Schistosomiasis mansoni / Q000175:diagnosis*
D000909:Antibodies, Helminth
Immunfluoreszenzserologische Befunde bei Schistosomiasis.--Methodische Aspekte.