Collagen-induced arthritis in mice. VI. Synovial cells from collagen arthritic mice activate autologous lymphocytes in vitro.
Synovial cells were extracted from normal and collagen-arthritic mice and investigated for lymphocyte-activating properties. In mixed cell culture, irradiated fibroblast-like synovial cells from DBA/1 LacJ arthritic mice stimulated a strong proliferative response in spleen cells from syngeneic normal mice, but not in cells from allogeneic DBA/2. B10.RIII, or BALB/c mice. This novel stimulus occurred in the absence of detectable Class II MHC antigen expression on the fibroblast-like synovial cell surface or increased autologous mixed lymphocyte reactions between DBA/1 LacJ spleen and lymph node cells. Irradiated synovial cells were also unable to present type II collagen to a collagen-specific T cell line and to stimulate proliferation. Addition of interferon-gamma or interleukin-1 failed to induce detectable surface Ia on the synovial fibroblasts or induce the capacity for antigen presentation in these cells.
Wooley
P H
PH
Center for the Rheumatic Diseases, Wayne State University Medical School, Detroit, Michigan 48201.
Whalen
J D
JD
Chapdelaine
J M
JM
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D002478:Cells, Cultured; D003094:Collagen / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D013154:Spleen / Q000166:cytology; D013583:Synovial Membrane / Q000166:cytology / Q000276:immunology*
D000949:Histocompatibility Antigens Class II; D007371:Interferon-gamma; D003094:Collagen; D007213:Indomethacin