Microplate selection technique (MPST). A new method for selecting mouse transfectants expressing human gene products.
This paper examines the analytical power of fluorescence activated cell sorting and immunorosetting technique as compared with the newly devised microplate selection technique in identifying transfected murine L cells expressing human surface molecules. The microplate selection technique relies on the mechanical transfer of transfected cells to a terasaki microplate, where an indirect immunofluorescence assay is carried out. It is a simple procedure not requiring costly equipment and with a detection capacity equivalent to that of the fluorescence activated cell sorter. The microplate selection technique proved to be sensitive enough to detect all the transfectants produced during the present study.
Peruzzi
L
L
Dipartimento di Genetica, Università di Torino, Turin, Italy.
Melioli
G
G
De Monte
L B
LB
Alessio
M
M
Zaccolo
M
M
Roggero
S
S
Bellone
G
G
Funaro
A
A
Malavasi
F
F
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000032:analysis*; D016827:CD8 Antigens; D002469:Cell Separation; D005434:Flow Cytometry; D007739:L Cells / Q000276:immunology; D051379:Mice; D011990:Receptors, Transferrin / Q000032:analysis; D012397:Rosette Formation; D014162:Transfection
D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D016827:CD8 Antigens; D011990:Receptors, Transferrin