Interaction of biofilm bacteria with antibiotics in a novel in vitro chemostat system.
Pseudomonas aeruginosa was cultivated at low growth rates under iron-limiting conditions on acrylic tiles. Biofilm cells exhibited increased tobramycin resistance compared with that of planktonic cells, and in old biofilms were more resistant than were cells in young biofilms. However, on suspension of the biofilm bacteria, glycocalyx-mediated resistance was lost.
Anwar
H
H
Department of Microbiology, University of Alberta, Edmonton, Canada.
van Biesen
T
T
Dasgupta
M
M
Lam
K
K
Costerton
J W
JW
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D004352:Drug Resistance, Microbial; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D014031:Tobramycin / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D014031:Tobramycin