Evidence for altered expression of the GTP-dependent regulatory proteins, Gs and Gi, in adipocytes from aged rats.
The alpha-subunits of Gi and Gs were quantified in adipocyte membranes from young (2-month) and older (18-month) rats by pertussis-toxin and cholera-toxin labelling respectively. Aging was associated with a 3-fold increase in Gi alpha-subunit, but only a 2-fold increase in one of the two Gs alpha-subunit species labelled. The findings may explain the altered sensitivity of adipocytes from aged rats to lipolytic and anti-lipolytic stimuli.
Green
A
A
Department of Internal Medicine, University of Texas Medical Branch, Galveston 77550.
Johnson
J L
JL
D000262:Adenylyl Cyclases / Q000378:metabolism; D000273:Adipose Tissue / Q000032:analysis* / Q000201:enzymology; D000375:Aging; D000818:Animals; D019204:GTP-Binding Proteins / Q000032:analysis*; D008297:Male; D008566:Membranes / Q000032:analysis / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D011983:Receptors, Purinergic / Q000032:analysis; D014118:Toxins, Biological
D011983:Receptors, Purinergic; D014118:Toxins, Biological; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases