Identification of RegA protein from Pseudomonas aeruginosa using anti-RegA antibody.
The product of Pseudomonas aeruginosa regA gene acts as a positive regulator of exotoxin A expression. The protein, RegA, was overproduced in E. coli transformed with an expression vector containing the regA gene. The overproduced RegA accumulated in E. coli in the form of inclusion bodies. The latter were isolated and served as a source of antigen for raising polyclonal antibodies. The antibodies reacted specifically with a P. aeruginosa protein whose molecular weight corresponded to that predicted for RegA from its known DNA sequence, and whose response to modulating factors matched that expected for RegA. The immunodetectable RegA was localized in the membrane fraction of P. aeruginosa strain PA103.
Zimniak
L
L
Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, NY 14642.
Dayn
A
A
Iglewski
B H
BH
D036002:ADP Ribose Transferases; D000936:Antigen-Antibody Complex / Q000032:analysis; D001427:Bacterial Toxins; D004926:Escherichia coli / Q000235:genetics; D005098:Exotoxins / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D005809:Genes, Regulator; D005822:Genetic Vectors; D007106:Immune Sera; D008970:Molecular Weight; D015345:Oligonucleotide Probes; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D000936:Antigen-Antibody Complex; D001427:Bacterial Toxins; D005098:Exotoxins; D007106:Immune Sera; D015345:Oligonucleotide Probes; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A