title: Characterization and localization of the sporulation glucoamylase of Saccharomyces cerevisiae.
abstract: Glucoamylase (SGA) was purified approximately 250-fold from sporulating Saccharomyces cerevisiae cells. The partially purified enzyme was active against glycogen, starch, maltotriose and maltose. It exhibited maximum catalytic activity against glycogen at pH 5.5. The enzyme appears to be glycosylated, because it bound to lentil-lectin Sepharose. SGA was expressed in vegetatively growing cells under the control of the GAL1 promoter, and the cellular location of the enzymatic activity determined by fractionation techniques. SGA was preferentially recovered in fractions which were enriched for the vacuolar hydrolases, carboxypeptidase Y and alpha-mannosidase.
authors.affiliation: Department of Biological Sciences, University of Notre Dame, IN 46556.
authors.forename: T A
authors.initials: TA
authors.lastname: Pugh
authors.forename: J C
authors.initials: JC
authors.lastname: Shah
authors.forename: P T
authors.initials: PT
authors.lastname: Magee
authors.forename: M J
authors.initials: MJ
authors.lastname: Clancy
mesh_terms: D002236:Carbohydrate Conformation; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D005087:Glucan 1,4-alpha-Glucosidase / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006003:Glycogen / Q000378:metabolism; D006031:Glycosylation; D006863:Hydrogen-Ion Concentration; D037102:Lectins / Q000378:metabolism; D008320:Maltose / Q000378:metabolism; D008970:Molecular Weight; D037121:Plant Lectins; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D013172:Spores, Fungal; D013213:Starch / Q000378:metabolism; D013379:Substrate Specificity; D014170:Transformation, Genetic; D014312:Trisaccharides / Q000378:metabolism; D014617:Vacuoles / Q000201:enzymology
chemical_list: D037102:Lectins; D037121:Plant Lectins; D014312:Trisaccharides; C033619:lentil lectin; C008317:maltotriose; D008320:Maltose; D013213:Starch; D006003:Glycogen; D005087:Glucan 1,4-alpha-Glucosidase