title: Localization of collagen in the rat lung: biochemical quantitation of types I and III collagen in small airways, vessels, and parenchyma.
abstract: The marked insolubility of pulmonary collagen has limited its accurate biochemical quantitation in small samples of lung and other tissues. We have recently developed a microassay based on radioisotope dilution techniques that we have used for the accurate determination of types I and III collagen in extremely small tissue samples. By applying this method to carefully dissected small airways and vessels and samples of parenchymal tissue of rat lungs, we have localized and quantitated biochemically the type I and III structural collagens of the lung. Large pulmonary arteries are the units richest in these interstitial collagen types on the basis of dried tissue weight (50 micrograms/100 micrograms dried tissue). Amounts of both types I and III collagen are considerably lower in the alveolar domain than in vessels and airways of the rat lung. The proportion of tissue composed of these collagen types decreases centripetally in rat pulmonary arteries, but increases in the bronchial tree. The relative proportions of type I and type III remain constant in all the structures tested. The higher total amount of collagen in the nonalveolar domain has implications for biochemical studies based on whole lung samples.
authors.affiliation: Department of Medicine, University of Vermont College of Medicine, Burlington 05405.
authors.forename: J
authors.initials: J
authors.lastname: Kelley
authors.forename: L
authors.initials: L
authors.lastname: Chrin
authors.forename: J T
authors.initials: JT
authors.lastname: Coflesky
authors.forename: J N
authors.initials: JN
authors.lastname: Evans
mesh_terms: D000818:Animals; D001671:Biochemistry / Q000379:methods; D001808:Blood Vessels / Q000378:metabolism; D003094:Collagen / Q000145:classification / Q000378:metabolism*; D008168:Lung / Q000378:metabolism*; D011650:Pulmonary Alveoli / Q000378:metabolism; D011651:Pulmonary Artery / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
chemical_list: D003094:Collagen