Pertussis toxin inhibits 1-methyladenine-induced maturation in starfish oocytes.
Starfish oocytes injected with pertussis toxin (3-6 micrograms/ml) or its catalytically active A-subunit (1 microgram/ml) did not undergo germinal vesicle breakdown in response to 1-methyladenine (1-10 microM). The pertussis block could be bypassed by transfer of cytoplasm that contained maturation-promoting factor (MPF). After insemination, pertussis-blocked, MPF-rescued oocytes underwent cortical vesicle exocytosis and cleavage. These results suggest the involvement of a pertussis sensitive G-protein in the pathway coupling 1-methyladenine action at the cell surface to the reinitiation of meiosis.
Shilling
F
F
Department of Biology, University of Southern California, Los Angeles 90089.
Chiba
K
K
Hoshi
M
M
Kishimoto
T
T
Jaffe
L A
LA
D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D003593:Cytoplasm; D005089:Exocytosis; D005260:Female; D019204:GTP-Binding Proteins / Q000502:physiology; D006133:Growth Substances / Q000494:pharmacology; D016200:Maturation-Promoting Factor; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D037342:Pertussis Toxin; D013215:Starfish; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
D006133:Growth Substances; D010566:Virulence Factors, Bordetella; C008407:1-methyladenine; D037342:Pertussis Toxin; D016200:Maturation-Promoting Factor; D019204:GTP-Binding Proteins; D000225:Adenine