Protein synthesis inhibition by cycloheximide does not affect the acute release of tissue-type plasminogen activator.
The acute release of tissue-type plasminogen activator (t-PA) was studied in perfused rat hindlegs. Pretreatment of rats with the protein syNthesis inhibitor cycloheximide (2 mg/kg) at 1, 3 or 5 h prior to perfusion of rat hindlegs did not influence the amount of t-PA released by platelet-activating factor (20 nM) or bradykinin (1 microM). The amount of t-PA activity that could be extracted from hindleg skeletal muscle was not decreased by cycloheximide pretreatment though it was decreased in lung extracts. The in vivo release of t-PA was not affected by cycloheximide pretreatment. The data suggest that the acute release of t-PA from vascular endothelial cells does not require ongoing protein synthesis, but that acutely released t-PA is derived from a stable endothelial storage pool.
Tranquille
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Gaubius Institute TNO, Leiden, The Netherlands.
Emeis
J J
JJ
D000818:Animals; D003513:Cycloheximide / Q000008:administration & dosage / Q000494:pharmacology*; D005342:Fibrinolysis; D006614:Hindlimb; D008168:Lung / Q000032:analysis; D008297:Male; D009132:Muscles / Q000032:analysis; D010477:Perfusion; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D010959:Tissue Plasminogen Activator / Q000378:metabolism*
D011500:Protein Synthesis Inhibitors; D003513:Cycloheximide; D010959:Tissue Plasminogen Activator