[Assay of biogenic amines by bioluminescence].
A bioluminescent procedure to measure noradrenaline and serotonin has been realized. The amines are oxidized by the monoamine oxidase of pig brain mitochondria. The NH3 generated in this reaction is directly measured by enzymatic reaction. The coenzyme of this last reaction is the NADH,H+ which is measured with a bioluminescent system: the FMN-oxidoreductase-luciferase. The extension to other amines is possible, it depends only of the specificity of the monoamine oxidase.
Fraysse
M
M
Laboratoire de Biochimie, Faculté de Pharmacie, Limoges.
Dumas
P
P
Nicot
G
G
Lachâtre
G
G
Habrioux
G
G
D001679:Biogenic Amines / Q000032:analysis*; D005486:Flavin Mononucleotide; D008156:Luciferases; D008163:Luminescent Measurements; D008928:Mitochondria / Q000201:enzymology; D008995:Monoamine Oxidase; D009243:NAD / Q000032:analysis; D009638:Norepinephrine / Q000032:analysis; D010088:Oxidoreductases; D012701:Serotonin / Q000032:analysis
D001679:Biogenic Amines; D009243:NAD; D012701:Serotonin; D005486:Flavin Mononucleotide; D010088:Oxidoreductases; D008156:Luciferases; D008995:Monoamine Oxidase; D009638:Norepinephrine
Dosage des amines biogènes par bioluminescence.