Hepatic synthesis of apoproteins of very low density and high density lipoproteins in perfused rat liver: influence of chronic heavy and moderate doses of ethanol.
The effects of 6 weeks of heavy and moderate ethanol feeding to rats upon lipids and lipoprotein metabolism were determined. As compared to the control group, the heavy ethanol feeding resulted in the following changes: liver weight/kilogram body weight increased by 48% (p less than 0.001) with a concomitant 52% increase (p less than 0.001) in liver protein/kilogram body weight and a 2.75-fold (p less than 0.001) increase in liver total lipids/kilogram body weight. In contrast, liver DNA/kilogram body weight or per liver was not affected significantly. Plasma cholesterol and triglycerides were higher by 53% (p less than 0.01) and 77% (p less than 0.01), respectively. Liver cholesterol and triglycerides were 4.4-fold and 3.8-fold higher (p less than 0.001), respectively. Plasma total A1 was 1.72-fold higher (p less than 0.001), whereas there was no significant difference in plasma apo E levels between the two groups. However, plasma high density lipoproteins (HDl) apo E was 48% lower (p less than 0.02) while the very low density lipoproteins (VLDL) E was 2.15-fold higher (p less than 0.02). Hepatic total protein synthetic rate in the ethanol group was not significantly different from the control group. In contrast, labeled leucine incorporation into the total secretory proteins was inhibited by 36% (p less than 0.01) in ethanol-fed group.(ABSTRACT TRUNCATED AT 250 WORDS)
Lakshman
M R
MR
Lipid Research Laboratory, VA Medical Center, Washington, DC 20422.
Chirtel
S J
SJ
Chambers
L C
LC
Campbell
B S
BS
D000437:Alcoholism / Q000097:blood*; D000818:Animals; D016632:Apolipoprotein A-I; D001053:Apolipoproteins / Q000097:blood*; D001054:Apolipoproteins A / Q000097:blood; D001057:Apolipoproteins E / Q000097:blood; D002784:Cholesterol / Q000097:blood; D004261:DNA Replication / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000494:pharmacology; D008075:Lipoproteins, HDL / Q000097:blood*; D008079:Lipoproteins, VLDL / Q000097:blood*; D008099:Liver / Q000187:drug effects; D008108:Liver Diseases, Alcoholic / Q000097:blood*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D014176:Protein Biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D014280:Triglycerides / Q000097:blood
D016632:Apolipoprotein A-I; D001053:Apolipoproteins; D001054:Apolipoproteins A; D001057:Apolipoproteins E; D008075:Lipoproteins, HDL; D008079:Lipoproteins, VLDL; D014280:Triglycerides; C025121:apolipoprotein E-rich HDL; C027610:apolipoprotein VLDL II; D000431:Ethanol; D002784:Cholesterol