Effect of handling of plasma samples on histamine radio-enzyme assay.
1. Freeze-thawing of plasma samples increased the histamine level at physiological histamine concentrations as analyzed by radio-enzyme assay, but not by high performance liquid chromatography. 2. Heating of plasma samples decreased the histamine levels. 3. Enzymatic or non-enzymatic formation of histamine during handling of plasma samples was not detected. 4. Some albumin preparations contain histamine.
Harvima
R J
RJ
Department of Dermatology, University of Kuopio, Finland.
Harvima
I T
IT
Kajander
E O
EO
Penttilä
I M
IM
Neittaanmäki
H
H
Fräki
J E
JE
D000328:Adult; D006631:Amine Oxidase (Copper-Containing); D004588:Electrophoresis, Cellulose Acetate; D005615:Freezing; D006632:Histamine / Q000097:blood*; D006640:Histidine Decarboxylase; D006358:Hot Temperature; D006801:Humans; D012016:Reference Values; D012588:Scintillation Counting / Q000379:methods; D013048:Specimen Handling
D006632:Histamine; D006631:Amine Oxidase (Copper-Containing); D006640:Histidine Decarboxylase