Underlying assumptions in the estimation of secondary structure content in proteins by circular dichroism spectroscopy--a critical review.
Recombinant DNA technology has made possible the large-scale production of proteins for pharmaceutical applications. As a result, there has been a renaissance in methodology which can provide information on the structural stability and character of these materials. Circular dichroism (CD) spectroscopy, with its sensitivity to the secondary structure adopted by the polypeptide chain, is a powerful tool in this regard. Quantitative analysis of the CD spectra of proteins is now wide-spread, aided by the availability of such algorithms on commercial instrumentation. However, there are basic assumptions made when conducting these calculations, many of which have not been addressed or summarized. Some of these assumptions are independent of the selection of basis spectra and the algorithm employed. These assumptions are listed and the available data concerning their validity is presented and discussed.
Manning
M C
MC
Department of Pharmaceutical Chemistry, University of Kansas, Lawrence 66045.
D055598:Chemical Phenomena; D002621:Chemistry; D002942:Circular Dichroism; D011487:Protein Conformation; D011506:Proteins / Q000032:analysis*
D011506:Proteins