Improved lung preservation using a dimethylthiourea flush.
The purpose of this study was to evaluate the ability of dimethylthiourea (DMTU), a low molecular weight hydroxyl free radical scavenger, to improve preservation of the lung for transplantation. Following preservation, 15 isolated canine left lower lobes were reperfused for 90 min with autologous blood. Five group I lobes served as controls and were not subjected to ischemia prior to reperfusion. Five group II lobes were flushed and submerged in a cold Euro-Collins solution and stored for 4 hr at 4 degrees C prior to reperfusion. Group III lobes were flushed with a 20 mM DMTU-enhanced Euro-Collins solution, stored for 4 hr, and then reperfused. The isogravimetric method was utilized to determine the capillary permeability coefficient (Kfc) for the reperfused lobes. The Kfc values were 0.10 +/- 0.01, 0.17 +/- 0.01, and 0.10 +/- 0.008 ml/min/mm Hg/100 g lung for groups I, II, and III, respectively (P less than 0.01 II vs I, III). Extravascular lung water values in the reperfused lobe were 4.44 +/- 0.45, 6.57 +/- 0.38, and 5.23 +/- 0.22 ml/g blood free dry lung weight for groups I, II, and III (P less than .05, II vs. I, III). Lung lipid peroxidation, measured as thiobarbituric acid-reactive material, was higher in group II, 146 +/- 6 nmole/g, than in either group I, 90 +/- 5 nmole/g, or group III, 91 +/- 4 nmole/g (P less than 0.01). The results indicate that the addition of DMTU improves hypothermic lung preservation by reducing lipid peroxidation and edema formation upon reperfusion.
Paull
D E
DE
Division of Cardiothoracic Surgery, University of North Carolina School of Medicine, Chapel Hill 27599.
Keagy
B A
BA
Kron
E J
EJ
Wilcox
B R
BR
D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects; D004285:Dogs; D005110:Extracellular Space / Q000187:drug effects; D005609:Free Radicals; D015227:Lipid Peroxidation / Q000187:drug effects; D008168:Lung / Q000098:blood supply / Q000187:drug effects; D016040:Lung Transplantation; D009926:Organ Preservation / Q000379:methods*; D010100:Oxygen / Q000378:metabolism; D015427:Reperfusion Injury / Q000517:prevention & control; D013890:Thiourea / Q000031:analogs & derivatives* / Q000494:pharmacology
D005609:Free Radicals; C038983:1,3-dimethylthiourea; D013890:Thiourea; D010100:Oxygen