Computerized morphometric differentiation of chromatin changes in splenocytes from normal and calorie-restricted mice.
Morphometric image analysis of splenocytes obtained from mice fed calorie-restricted diets for periods of up to three weeks revealed slight but significant changes in nuclear size, nuclear texture and total amounts of stainable Feulgen-positive DNA as compared to age-and-sex-matched control cells. The cellular changes were associated with body weight loss and changes in the distribution of splenic T cells and B cells during the three weeks of dietary restriction. The reduced nuclear area and Feulgen-stained DNA may reflect an altered susceptibility of cells from nutrient-restricted sources toward acid hydrolysis. This in turn may be indicative of a temporarily altered synthesis of subcellular components, as these phenomena seem to lessen by the third week of dietary restriction.
Olson
G B
GB
Department of Microbiology/Immunology, University of Arizona, Tucson 85721.
Jolley
C S
CS
Olson
K M
KM
Bruno
J G
JG
D000818:Animals; D002467:Cell Nucleus / Q000473:pathology; D002843:Chromatin / Q000473:pathology*; D004247:DNA / Q000032:analysis; D007091:Image Processing, Computer-Assisted; D008297:Male; D051379:Mice; D011502:Protein-Energy Malnutrition / Q000378:metabolism / Q000473:pathology*; D013154:Spleen / Q000473:pathology
D002843:Chromatin; D004247:DNA