Identification of GTP-binding proteins in Fasciola hepatica and Schistosoma mansoni by immunoblotting.
Both the liver fluke Fasciola hepatica and the blood fluke Schistosoma mansoni have GTP-binding proteins which are part of the trans-membrane signalling system. These proteins require GTP in order to interact with the catalytic subunit of adenylate cyclase. The technique of immunoblotting was used in order to distinguish the GTP-binding proteins Gs, Gi, and Go. Immunoblotting was carried out using antisera prepared against peptides deduced from bovine cDNA clones specific for alpha or beta subunits of known G proteins. A 41-kDa Gs alpha has been identified in S. mansoni and a 42.5-kDa Gs alpha in F. hepatica. A 41-kDa Go alpha was found in both parasites. A 36-kDa G beta was identified in both parasites using antiserum made against bovine transducin.
Mansour
J M
JM
Department of Pharmacology, Stanford University School of Medicine, CA 94305.
Mansour
T E
TE
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000469:parasitology; D004247:DNA / Q000276:immunology; D005210:Fasciola hepatica / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D005260:Female; D019204:GTP-Binding Proteins / Q000032:analysis*; D015151:Immunoblotting / Q000379:methods; D008297:Male; D051379:Mice; D008970:Molecular Weight; D012550:Schistosoma mansoni / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D015293:Transducin / Q000276:immunology
D004247:DNA; D019204:GTP-Binding Proteins; D015293:Transducin