[Immunocytochemical localization of cytochrome P-450scc in cultured rat oligodendrocytes].
Primary cultures of glial cells from newborn rat forebrain were tested after 3 to 4 weeks. Oligodendrocytes and astrocytes were characterized by immunofluorescence with monoclonal antibodies to galactocerebroside and glial fibrillary acidic protein, respectively. The cytoplasm of oligodendrocytes was specifically and intensely immunostained with monospecific polyclonal antibodies to the cytochrome P-450scc involved in the synthesis of pregnenolone from cholesterol. This observation brings additional support to the concept of "neurosteroids".
Jung-Testas
I
I
Unité 33, I.N.S.E.R.M., Lab. Hormones, Bicêtre.
Alliot
F
F
Pessac
B
B
Robel
P
P
Baulieu
E E
EE
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis*; D003593:Cytoplasm / Q000032:analysis; D005455:Fluorescent Antibody Technique; D009457:Neuroglia / Q000032:analysis*; D009836:Oligodendroglia / Q000032:analysis*; D051381:Rats
D003577:Cytochrome P-450 Enzyme System
Localisation immunocytochimique du cytochrome P-450scc dans les oligodendrocytes de rat en culture.