title: Cytokines produced in lymph follicles.
abstract: The events occurring inside lymph follicles during a germinal center reaction are poorly understood. Using B and T lymphoid cell populations prepared from human tonsillar lymph follicles, and enriched or not in macrophages or in follicular dendritic cells, we examined the production of cytokines by these cells in vitro. Interleukin 6 (IL-6) and tumor necrosis factor (TNF) were found in the supernatants of cultures stimulated with phytohemagglutinin or pokeweed mitogen. IL-1 beta was occasionally detected; its secretion apparently depends on the origin of the tonsils, the stimulation, and the cell populations. IFN-gamma and IL-2 were not produced in significant amounts by these lymph follicle cells. IL-4 was only found in very low concentrations in the supernatant of the different cell cultures. The cell populations containing follicular dendritic cells produced more IL-6 and TNF than the others, especially than those composed of only B and T cells.
authors.affiliation: Institute of Clinical Medicine, University of Tsukuba, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Tsunoda
authors.forename: N
authors.initials: N
authors.lastname: Cormann
authors.forename: E
authors.initials: E
authors.lastname: Heinen
authors.forename: K
authors.initials: K
authors.lastname: Onozaki
authors.forename: P
authors.initials: P
authors.lastname: Coulie
authors.forename: Y
authors.initials: Y
authors.lastname: Akiyama
authors.forename: K
authors.initials: K
authors.lastname: Yoshizaki
authors.forename: C
authors.initials: C
authors.lastname: Kinet-Denoël
authors.forename: L J
authors.initials: LJ
authors.lastname: Simar
authors.forename: M
authors.initials: M
authors.lastname: Kojima
mesh_terms: D000234:Adenoids / Q000166:cytology* / Q000378:metabolism; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D003713:Dendritic Cells / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000032:analysis; D007378:Interleukins / Q000378:metabolism*; D008214:Lymphocytes / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D014066:Palatine Tonsil / Q000166:cytology* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D007378:Interleukins; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma