title: Detection of extended broad-spectrum beta-lactamases in Enterobacteriaceae in four French hospitals.
abstract: In 210 strains of Enterobacteriaceae which were isolated in four hospitals and which showed reduced susceptibility to cefotaxime, high synergy was demonstrated between amoxicillin (20 micrograms) + clavulanate (10 micrograms) and cefotaxime (30 micrograms) using a simple double-disk test. Isoelectric focusing on gel and specific iodometric detection using ceftriaxone identified four extended broad-spectrum beta-lactamases (isoelectric points 7.6, 6.3, 7.0 and 5.9) produced by the strains.
authors.affiliation: Service de Bactériologie, Hôpital Henri Mondor, Créteil, France.
authors.forename: P
authors.initials: P
authors.lastname: Legrand
authors.forename: G
authors.initials: G
authors.lastname: Fournier
authors.forename: A
authors.initials: A
authors.lastname: Buré
authors.forename: V
authors.initials: V
authors.lastname: Jarlier
authors.forename: M H
authors.initials: MH
authors.lastname: Nicolas
authors.forename: D
authors.initials: D
authors.lastname: Decré
authors.forename: J
authors.initials: J
authors.lastname: Duval
authors.forename: A
authors.initials: A
authors.lastname: Philippon
mesh_terms: D000658:Amoxicillin / Q000494:pharmacology*; D019980:Amoxicillin-Potassium Clavulanate Combination; D002439:Cefotaxime / Q000494:pharmacology*; D002954:Citrobacter / Q000187:drug effects / Q000201:enzymology / Q000302:isolation & purification; D002969:Clavulanic Acids / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D004357:Drug Synergism; D004359:Drug Therapy, Combination / Q000494:pharmacology; D004754:Enterobacter / Q000187:drug effects / Q000201:enzymology / Q000302:isolation & purification; D004755:Enterobacteriaceae / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000302:isolation & purification; D005602:France; D006801:Humans; D008826:Microbial Sensitivity Tests; D010403:Penicillin Resistance; D012706:Serratia marcescens / Q000187:drug effects / Q000201:enzymology / Q000302:isolation & purification; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D002969:Clavulanic Acids; D019980:Amoxicillin-Potassium Clavulanate Combination; D000658:Amoxicillin; D001618:beta-Lactamases; D002439:Cefotaxime