A successful model of small bowel autotransplantation in the dog.
Experimental small bowel transplantation has continued to be a complex procedure with high mortality. We investigated the technical aspects of small bowel transplantation in an effort to define a procedure that would result in an improved survival rate. Three methods of graft harvesting were examined in a model of canine small bowel autotransplantation. Harvesting the graft by first flushing with room-temperature lactated Ringer's followed by iced lactated Ringer's resulted in the best preservation and subsequently the best survival rate (71%). Flushing with iced lactated Ringer's alone resulted in survival rates of 22 and 50% in two additional groups. We also investigated two methods of graft reanastomosis. Although either venous drainage regimen appears to be suitable, graft venous reanastomosis to the host portal vein resulted in a slightly higher postoperative weight than reanastomosis to the host inferior vena cava. A model of small bowel transplantation with a high long-term survival rate has been developed. This model can now be applied to studies of the various physiological aspects of small bowel transplantation.
Raju
S
S
Department of Surgery, University of Mississippi Medical Center, Jackson, MS 39216-4505.
Fujiwara
H
H
Grogan
J B
JB
Dzielak
D J
DJ
D000818:Animals; D003967:Diarrhea / Q000209:etiology; D004285:Dogs / Q000601:surgery*; D005260:Female; D006085:Graft Survival; D007421:Intestine, Small / Q000637:transplantation*; D008297:Male; D009926:Organ Preservation; D011183:Postoperative Complications / Q000401:mortality; D013927:Thrombosis / Q000209:etiology / Q000401:mortality; D014182:Transplantation, Autologous / Q000379:methods