The phosphatidylinositol response of chondrocytes in culture.
This study examined the phosphatidylinositol (PI) response in chondrocytes. The levels of 3H-inositol phosphates (IPs) were measured in guinea pig chondrocyte cultures labelled with 3H-myo-inositol and exposed to various inflammatory mediators and known agonists of the PI response, including bradykinin, carbachol, histamine, A23187, or recombinant human interleukin-1 beta. Of the mediators used to stimulate the PI response in chondrocytes, only bradykinin was effective. The bradykinin treated cells had higher levels of IPs (37-fold) than the controls. The PI response to bradykinin was dose and time dependent.
Baragi
V M
VM
Parke-Davis Pharm. Res. Div., Warner-Lambert Co., Ann Arbor, MI 48105.
Dudley
D T
DT
Schrier
D J
DJ
D000818:Animals; D001920:Bradykinin / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D002358:Cartilage, Articular / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology; D010716:Phosphatidylinositols / Q000096:biosynthesis*; D013268:Stimulation, Chemical
D010716:Phosphatidylinositols; D000001:Calcimycin; D006632:Histamine; D002217:Carbachol; D001920:Bradykinin