Cellular communication in leukotriene C4 production between eosinophils and neutrophils.
Eosinophilic granulocytes as well as neutrophilic granulocytes produced leukotriene C4 (LTC4) on stimulation with 1 microM A23187 (Ca2+ ionophore). In healthy volunteers, the LTC4 production in eosinophils was about 3 times the production in neutrophils. Within 15 min greater than 90% of the LTC4 was released into the supernatant. Stimulation of eosinophils and neutrophils together resulted in a synergistic increase in LTC4 production of 306 +/- 40%. LTC4 synthesis by hypodense eosinophils was also enhanced if stimulated in the presence of neutrophils. These findings suggest a communication between eosinophils and neutrophils, which may play a role in bronchial asthma.
Kloprogge
E
E
Department of Allergology, University Hospital, Groningen, The Netherlands.
de Leeuw
A J
AJ
de Monchy
J G
JG
Kauffman
H F
HF
D001249:Asthma / Q000276:immunology / Q000378:metabolism; D000001:Calcimycin / Q000494:pharmacology; D002450:Cell Communication; D015267:Churg-Strauss Syndrome / Q000276:immunology / Q000378:metabolism; D004804:Eosinophils / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007958:Leukocyte Count; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism*; D013189:SRS-A / Q000096:biosynthesis*
D013189:SRS-A; D000001:Calcimycin