Rapid modulation of a 64 K dalton fibroblast protein: a PDGF mediated early cellular event.
The results presented here reveal a novel platelet derived growth factor (PDGF) mediated early cellular event. Treatment of growth arrested Balb/c3T3 fibroblasts with PDGF induces a specific and rapid modulation of a 64,000 Dalton (64 KD) protein preexisting in quiescent cells. The kinetics of 64 KD protein modulation indicate that, temporally, this PDGF mediated step lies between the membrane associated immediate events such as receptor autophosphorylation or ion mobilization and the earliest known transcriptional event, the activation of the proto-oncogene c-fos.
Singh
J P
JP
Metabolic Diseases Research and Biotechnology-Biopolymer, Upjohn Company, Kalamazoo, MI 49001.
Bonin
P D
PD
Adams
L D
LD
D000818:Animals; D002460:Cell Line; D003470:Culture Media / Q000032:analysis; D004247:DNA / Q000096:biosynthesis; D015180:Electrophoresis, Gel, Two-Dimensional; D005347:Fibroblasts / Q000378:metabolism* / Q000502:physiology; D005786:Gene Expression Regulation; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos; D014158:Transcription, Genetic / Q000187:drug effects
D003470:Culture Media; D010982:Platelet-Derived Growth Factor; D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D004247:DNA