Effect of treatment with phospholipase A2, colchicine, and beta-galactosidase on agglutination of rat hepatocytes.
The effects of phospholipase A2, colchicine, and beta-galactosidase on concanavalin A-induced agglutination of viable hepatocytes isolated from normal and diabetic rats are reported. Phospholipase A2 (0.92 microgram/mL), colchicine (400 micrograms/mL), and beta-galactosidase (300 micrograms/mL) treatments caused a significant increase in the agglutination rate of hepatocytes. These findings suggest that phospholipase A2 treatment resulted in the unshielding of lectin receptors. Colchicine treatment seemed to release those receptors from cellular restraints which tend to separate and/or direct them. The promoting effect of beta-galactosidase could be attributed to a decrease in repulsive forces due to a reduction in net negative charge density after removal of N-acetylneuraminic acid residues. Normal rat hepatocytes seem to be richer in galactosides, phospholipids, and the microfilament-microtubule network than their diabetic counterparts.
Bikhazi
A B
AB
Department of Physiology, Faculty of Medicine, American University of Beirut, Lebanon.
Saad
S H
SH
Ghaleb
T A
TA
D000371:Agglutination; D000818:Animals; D003078:Colchicine / Q000494:pharmacology*; D003208:Concanavalin A / Q000494:pharmacology; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism; D005696:Galactosidases / Q000494:pharmacology*; D008099:Liver / Q000166:cytology* / Q000187:drug effects; D010740:Phospholipases / Q000494:pharmacology*; D010741:Phospholipases A / Q000494:pharmacology*; D054467:Phospholipases A2; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D001616:beta-Galactosidase / Q000494:pharmacology*
D003208:Concanavalin A; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D005696:Galactosidases; D001616:beta-Galactosidase; D003078:Colchicine