Cell proliferation and expression of cytokeratin filaments in F9 embryonal carcinoma cells.
A double immunofluorescence method was developed for the monitoring of proliferation and differentiation of F9 embryonal carcinoma cells. Cytokeratin filament expression was used as a marker for differentiation, and proliferating cell nuclear antigen (PCNA)/cyclin or bromodeoxyuridine labeling were used as markers for proliferation. F9 cells had a high proliferation rate and were cytokeratin-filament-negative. Upon treatment with retinoic acid and dibutyryl cyclic AMP, cytokeratin-filament-positive cells with differentiated phenotype appeared. After 3 days, the extent of proliferation of cytokeratin-filament-positive cells was comparable to, but after 5 days significantly lower than, that of cytokeratin-filament-negative cells in the same culture. In differentiating F9 cells, cytokeratin filament expression is associated with, and even slightly precedes, the dramatic decrease in the rate of proliferation.
Kurki
P
P
Department of Pathology, University of Helsinki, Finland.
Laasonen
A
A
Tan
E M
EM
Lehtonen
E
E
D008841:Actin Cytoskeleton / Q000502:physiology / Q000648:ultrastructure*; D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis; D003994:Bucladesine / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D007633:Keratins / Q000032:analysis / Q000096:biosynthesis*; D051379:Mice; D009687:Nuclear Proteins / Q000032:analysis / Q000096:biosynthesis; D018809:Proliferating Cell Nuclear Antigen; D013724:Teratoma; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; D014212:Tretinoin; D003994:Bucladesine; D007633:Keratins