Dengue virus-specific cross-reactive CD8+ human cytotoxic T lymphocytes.
Stimulation with live dengue virus of peripheral blood mononuclear cells from a dengue virus type 4-immune donor generated virus-specific, serotype-cross-reactive, CD8+, class I-restricted cytotoxic T lymphocytes (CTL) capable of lysing dengue virus-infected cells and cells pulsed with dengue virus antigens of all four serotypes. These CTL lysed autologous fibroblasts infected with vaccinia virus-dengue virus recombinant viruses containing the E gene or several nonstructural dengue virus type 4 genes. These results demonstrate that both dengue virus structural and nonstructural proteins are targets for the cytotoxic T-cell-mediated immune response to dengue virus and suggest that serotype-cross-reactive CD8+ CTL may be important mediators of viral clearance and of virus-induced immunopathology during secondary dengue virus infections.
Bukowski
J F
JF
Department of Medicine, University of Massachusetts Medical Center, Worcester 01655.
Kurane
I
I
Lai
C J
CJ
Bray
M
M
Falgout
B
B
Ennis
F A
FA
D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000276:immunology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology; D016827:CD8 Antigens; D002460:Cell Line; D003165:Complement System Proteins / Q000276:immunology; D003429:Cross Reactions; D003602:Cytotoxicity, Immunologic; D003716:Dengue Virus / Q000276:immunology* / Q000528:radiation effects; D006801:Humans; D008213:Lymphocyte Activation; D012867:Skin / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014466:Ultraviolet Rays
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D000956:Antigens, Viral; D016827:CD8 Antigens; D003165:Complement System Proteins