Cyclooxygenase and lipoxygenase inhibitors act differently on oxidative product formation by immune mononuclear cells: a flow cytometric investigation.
The action of indomethacin, a cyclooxygenase inhibitor, and nordihydroguaiaretic acid (NDGA), a lipoxygenase inhibitor, on oxidative products formed by immune mononuclear cells was studied by flow cytometry. Jurkat T-cells and peripheral blood mononuclear cells were incubated with 2',7'-dichlorofluorescin diacetate; this substance is hydrolyzed in the cells leading to non-fluorescent 2',7'-dichlorofluorescin which is oxidized by oxygen reactive species into highly fluorescent 2,7'-dichlorofluorescein. Using this fluorescent probe, the formation of oxygen reactive species in phytohemagglutinin-stimulated mononuclear cells treated by NDGA or indomethacin was followed by flow cytometry. We observed that NDGA caused a marked decrease, both in the level of fluorescence intensity and in the number of fluorescent cells, whereas indomethacin caused a small increase in fluorescence intensity. On the other hand, NDGA inhibited and indomethacin increased the incorporation of tritiated thymidine by phytohemagglutinin-stimulated lymphocytes. These results suggest that oxygen reactive species are involved in the stimulation of immune mononuclear cells.
Rouahi
N
N
Laboratoire de Biochimie des Membranes, CNRS ER 228, Montpellier, France.
Levallois
C
C
Favier
F
F
Mani
J C
JC
D002478:Cells, Cultured; D016861:Cyclooxygenase Inhibitors; D005434:Flow Cytometry; D005453:Fluorescence; D006801:Humans; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D016859:Lipoxygenase Inhibitors; D009637:Masoprocol / Q000494:pharmacology; D009000:Monocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D010084:Oxidation-Reduction; D010835:Phytohemagglutinins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism
D016861:Cyclooxygenase Inhibitors; D016859:Lipoxygenase Inhibitors; D010835:Phytohemagglutinins; D009637:Masoprocol; D013936:Thymidine; D007213:Indomethacin