Automated fluoroimmunoassay of theophylline and valproic acid by flow-injection analysis with use of HPLC instruments.
For automated determination of theophylline and valproic acid by use of Ames' fluoroimmunoassays we used a high-pressure liquid-chromatographic system consisting of a pump, a robotic unit (Gilson 231/401, to prepare and inject the samples into the flowing carrier), and a fluorometer with a 10-microL flow cell. Results correlated well with those of conventional liquid- and gas-chromatography (r less than 0.96). The between-run CV is about 5%. In comparison with the manual method, the volume of reagents (and thus the cost per analysis) was decreased by eightfold.
Allain
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P
Laboratoire de Pharmacologie, C.H.U., Angers, France.
Turcant
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A
Prémel-Cabic
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A
D001322:Autoanalysis; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D005456:Fluorescent Dyes; D006801:Humans; D007118:Immunoassay; D012044:Regression Analysis; D013806:Theophylline / Q000097:blood*; D014635:Valproic Acid / Q000097:blood*
D005456:Fluorescent Dyes; D014635:Valproic Acid; D013806:Theophylline