Quantitation of the benzodiazepine antagonist flumazenil in human plasma by gas chromatography-mass spectrometry.
A gas chromatographic-mass spectrometric procedure has been developed for the quantitation of the benzodiazepine antagonist flumazenil in human plasma. The assay utilizes an extraction at alkaline pH with benzene-dichloroethane (80:20), selective ion monitoring, isobutane positive-ion chemical ionization mass spectrometry and stable isotope dilution. The method has been used to measure plasma concentrations of flumazenil in over 1500 clinical samples over a range of 0.5-200 ng/ml (using 2 ml of plasma).
Fukuda
E K
EK
Department of Drug Metabolism, Hoffmann-La Roche Inc., Nutley, NJ 07110.
Choma
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N
Davis
P P
PP
D001569:Benzodiazepines / Q000037:antagonists & inhibitors*; D005442:Flumazenil / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans
D001569:Benzodiazepines; D005442:Flumazenil