Clinical applications of luminescent assays for enzymes and enzyme labels.
A wide range of luminescent assays for enzyme labels has been developed. The clinical application of luminescent assays for horseradish peroxidase, alkaline phosphatase, xanthine oxidase, beta-D galactosidase, and glucose 6-phosphate dehydrogenase is discussed. The enzyme luminescent methods provide improved sensitivity compared with colorimetric and fluorimetric assays. A new reagent, dioxetane phosphate, has provided a simple and extremely sensitive assay for alkaline phosphatase. The available instruments and new photographic detection techniques for luminescent assays are briefly discussed.
Bronstein
I
I
Tropix Inc., Bedford, Massachusetts, MA 01730.
Kricka
L J
LJ
D000469:Alkaline Phosphatase / Q000032:analysis; D004798:Enzymes / Q000032:analysis*; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D006735:Horseradish Peroxidase / Q000032:analysis; D007124:Immunoenzyme Techniques; D008163:Luminescent Measurements; D008165:Luminol / Q000378:metabolism; D014969:Xanthine Oxidase / Q000032:analysis; D001616:beta-Galactosidase / Q000032:analysis
D004798:Enzymes; D008165:Luminol; D005954:Glucosephosphate Dehydrogenase; D006735:Horseradish Peroxidase; D014969:Xanthine Oxidase; D000469:Alkaline Phosphatase; D001616:beta-Galactosidase