Introduction of macromolecules into primary cultured neuronal cells by fusion with erythrocyte ghosts.
The macromolecules were injected into primary cultured neuronal cells by fusion with human erythrocyte ghosts containing macromolecules. The injection efficiency was about 30% and 80% of the cells extended neurites like normal neuronal cells. In order to succeed in this method, phytohaemagglutinin E and polyethylene glycol 1000 were essential, and monolayer culture cells were used.
Hikawa
N
N
Department of Physiology, Yokohama City University School of Medicine, Japan.
Horie
H
H
Kawakami
T
T
Okuda
K
K
Takenaka
T
T
D002459:Cell Fusion / Q000187:drug effects; D002478:Cells, Cultured; D004910:Erythrocyte Membrane / Q000378:metabolism*; D005727:Ganglia, Spinal / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000378:metabolism*; D007142:Immunoglobulin gamma-Chains / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D010835:Phytohemagglutinins; D011092:Polyethylene Glycols; D012710:Serum Albumin, Bovine / Q000378:metabolism*
D007143:Immunoglobulin Heavy Chains; D007142:Immunoglobulin gamma-Chains; D010835:Phytohemagglutinins; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols