Rapid determination of Trypanosoma cruzi urinary antigens in human chronic Chagas disease by agglutination test.
Detection of Trypanosoma cruzi in man becomes particularly difficult during the chronic stage of Chagas disease because of the low parasitemia. We were able to develop a simple and straightforward method for determining the concentration of T. cruzi antigens in urine using nitrocellulose micellar suspension (Nitrocell-Mr, Polychaco Argentina) and for their subsequent detection through a "latex" type agglutination test. The latex used was an esferocell nitrocellulose suspension (Esferocell-Mr, Polychaco). Specific antigens for T. cruzi were detected in 54 of 58 urine samples from chronic chagasic patients. The antigens characterized by affinity chromatography and SDS-PAGE were glycoproteins with apparent molecular weights (and pIs) of 100 kDa (pI 5 to 5.5), 80 kDa (pI 6.0), and 50 kDa (pI 6.5 to 7.0). This method is practical and fulfills the requirement of large-scale epidemiological studies. It is also helpful in cases of conflictive serology.
Katzin
A M
AM
Catedra de Microbiologia, Parasitologia e Inmunologia, Facultad de Medicina, UBA, Buenos Aires, Argentina.
Marcipar
A
A
Freilij
H
H
Corral
R
R
Yanovsky
J F
JF
D000818:Animals; D000953:Antigens, Protozoan / Q000652:urine*; D014355:Chagas Disease / Q000175:diagnosis*; D002846:Chromatography, Affinity; D002908:Chronic Disease; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007841:Latex Fixation Tests; D011233:Precipitin Tests; D011237:Predictive Value of Tests; D014349:Trypanosoma cruzi / Q000276:immunology*
D000953:Antigens, Protozoan; D003101:Collodion