[Cloning and expression of the determinant for resistance to a new class of tetracycline in Escherichia coli strains].
Tetracycline-resistance determinant of the plasmid pBS221 isolated from a Pseudomonas aeruginosa strain has been cloned on pUC19 vector plasmid. The determinant is expressed under aerobic and anaerobic conditions coding for two proteins: a 36 Kd protein conferring antibiotic resistance and 27 Kd repressor protein. The determinant is not homologous to tet-determinants of the known classes as shown by blot hybridization experiments. The determinant represents a new class--G. Determinants of the new class are widespread among Serratia marcescens strains.
Parfenova
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D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D006090:Gram-Negative Bacteria / Q000235:genetics; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000235:genetics; D013753:Tetracycline Resistance / Q000235:genetics*
Klonirovanie i ékspressiia determinanta rezistentnosti k tetratsiklinnovogo klassa v shtammakh Escherichia coli.