title: [Benzidine and its derivatives in the immunohistochemical analysis of peroxidase].
abstract: Authors have studied the application of benzidine and its derivatives for the demonstration of the marker enzyme horseradish peroxidase. Benzidine compounds are known to be in a blueish dimeric form under oxidative circumstances, while after polymerization they yield a dark brown end product. If the dimeric state is stabilized, the 3,3'-dimethoxy benzidine (o-dianisidin) appears to be the most suitable to demonstrate peroxidase. The o-dianisidin reaction has the advantage of high sensitivity and that the end product shows a birefringence in polarized light. Upon mounting with organic media the end product is stable.
authors.forename: G
authors.initials: G
authors.lastname: Mátyás
authors.forename: V
authors.initials: V
authors.lastname: Reisz
mesh_terms: D001560:Benzidines / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D007147:Immunoglobulin Light Chains / Q000032:analysis; D007150:Immunohistochemistry
chemical_list: D001560:Benzidines; D007147:Immunoglobulin Light Chains; C029876:benzidine
vernacular_title: Benzidin és származékai a peroxidáz enzim immunhisztokémiai kimutatására.