title: Induction of class I major histocompatibility complex antigen expression by West Nile virus on gamma interferon-refractory early murine trophoblast cells.
abstract: Primary murine trophoblast giant cells (TGC) do not express detectable major histocompatibility complex (MHC) antigens and are refractory to the MHC-increasing effects of alpha and beta (virus-induced) interferons and gamma (immune type) interferon during early implantation (postcoital days 3.5-6). West Nile virus infection of primary TGC monolayers from postcoital-day-3.5 preimplantation blastocysts induced paternal MHC antigen expression within 16 hr, as detected by immunogold labeling for electron microscopy. Induction is unlikely to have been mediated by secreted virus-induced interferons or other factors, as it occurred in the presence of high concentrations of anti-alpha/beta interferon antibodies and was not induced by virus-inactivated supernatants from MHC-induced primary TGC cultures. Attempts to induce MHC antigen expression with poly(I.C) or recombinant tumor necrosis factor alpha in primary TGC cultures also failed. Thus, the apparent inhibition of MHC antigen expression in primary TGC during early implantation and their refractoriness to induction of de novo MHC antigen expression is not absolute. This may represent a maternal-and/or species-protective evolutionary device. As such, manipulation of this phenomenon may allow a conclusive assessment of the significance of inhibition of MHC antigen expression on trophoblast cells in the implanting semiallogeneic embryo.
authors.affiliation: Department of Microbiology, John Curtin School of Medical Research, Australian National University, Canberra.
authors.forename: N J
authors.initials: NJ
authors.lastname: King
authors.forename: L E
authors.initials: LE
authors.lastname: Maxwell
authors.forename: A M
authors.initials: AM
authors.lastname: Kesson
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D005805:Genes, MHC Class I; D006680:HLA Antigens / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron; D011070:Poly I-C / Q000494:pharmacology; D011994:Recombinant Proteins; D014327:Trophoblasts / Q000187:drug effects / Q000276:immunology* / Q000648:ultrastructure; D014709:Vero Cells; D014902:West Nile virus / Q000235:genetics*
chemical_list: D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D011994:Recombinant Proteins; D007371:Interferon-gamma; D011070:Poly I-C