title: Thioredoxin is essential for photosynthetic growth. The thioredoxin m gene of Anacystis nidulans.
abstract: We have taken advantage of the transformation properties of the cyanobacterium Anacystis nidulans R2 to investigate the importance of thioredoxin for photosynthetic growth. The gene encoding thioredoxin m, designated trxM, was cloned from A. nidulans using a synthetic oligonucleotide probe. Based on the nucleotide sequence, thioredoxin m of A. nidulans is composed of 107 amino acids and shares 84, 48, and 48% sequence identity with thioredoxins from Anabaena, spinach, and Escherichia coli, respectively. The trxM gene is single copy and is transcribed on a 510-nucleotide mRNA. We demonstrate that disruption of the trxM gene with a kanamycin resistance gene cartridge is a lethal mutation. Although dispensable in E. coli, thioredoxin is essential for the photosynthetic growth of A. nidulans.
authors.affiliation: Division of Molecular Plant Biology, University of California, Berkeley 94720.
authors.forename: E G
authors.initials: EG
authors.lastname: Muller
authors.forename: B B
authors.initials: BB
authors.lastname: Buchanan
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000502:physiology*; D001483:Base Sequence; D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000502:physiology*; D004252:DNA Mutational Analysis; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005796:Genes; D005804:Genes, Lethal; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010788:Photosynthesis; D010957:Plasmids; D015183:Restriction Mapping; D013879:Thioredoxins / Q000502:physiology*; D014170:Transformation, Genetic
chemical_list: D001426:Bacterial Proteins; D015345:Oligonucleotide Probes; D013879:Thioredoxins