[Antigenic properties of the electrophoretic fractions of the causative agent of melioidosis].
Antisera to the antigens of 5 fractions, isolated as the result of the separation of P. pseudomallei aqueous saline extract by continuous electrophoresis in the vertical block of granulated gel, have been obtained. Immunoelectrophoresis with the use of P. pseudomallei aqueous saline extract has revealed that antisera to electrophoretic fractions contain antibodies mainly to the antigens of the corresponding fractions, which shows that this technique ensures the effective separation of P. pseudomallei biopolymers by their electrophoretic motility and molecular weight. These antisera differ in their species specificity. Thus, antisera to antigens with anode motility have been found to contain antibodies mainly to P. pseudomallei antigens and antisera to electroneutral antigens or to those with cathode motility, to P. pseudomallei and P. mallei antigens.
Barkov
A M
AM
Perov
V Iu
VIu
Pushkar'
V G
VG
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D000942:Antigens, Bacterial / Q000276:immunology* / Q000302:isolation & purification; D004306:Dose-Response Relationship, Immunologic; D015180:Electrophoresis, Gel, Two-Dimensional; D005455:Fluorescent Antibody Technique; D007114:Immunization; D005779:Immunodiffusion; D008554:Melioidosis / Q000175:diagnosis*; D011549:Pseudomonas / Q000276:immunology*; D011817:Rabbits
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial
Antigennye svoĭstva élektroforeticheskikh fraktsiĭ vozbuditelia melioidoza.