N-terminal sequence extension in the glycosylated forms of human pancreatic stone protein. The 5-oxoproline N-terminal chain is O-glycosylated on the 5th amino acid residue.
The pancreatic stone protein isolated from human calculi (PSP) derives from the immunoreactive protein forms detected in human pancreatic juice (PSP S2-5) through the tryptic cleavage of the Arg-11-Ile-12 bond. Among the eleven amino acids of the PSP S2-5 N-terminal extension Z-E-A-Q-T-E-L-P-Q-A-R, the first residue is an oxoproline and the fifth, a threonine, bears the single carbohydrate chain of the protein molecules. Variations in the glycan chain composition account for the differences in the Mr of PSP S2-5. The PSP S2-5 forms are very soluble in aqueous solutions between the pH values 5.0-9.0, whereas the proteolysated form is scarcely soluble.
De Caro
A M
AM
Unité de Recherches de Physiologie et de Pathologie Digestive, Marseille, France.
Adrich
Z
Z
Fournet
B
B
Capon
C
C
Bonicel
J J
JJ
De Caro
J D
JD
Rovery
M
M
D000116:Acetylgalactosamine / Q000032:analysis; D000117:Acetylglucosamine / Q000032:analysis; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002135:Calcium-Binding Proteins / Q000032:analysis / Q000378:metabolism; D002241:Carbohydrates / Q000032:analysis; D006031:Glycosylation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D051847:Lithostathine; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D010189:Pancreatic Juice / Q000032:analysis; D010446:Peptide Fragments / Q000378:metabolism; D010750:Phosphoproteins; D011760:Pyrrolidinones; D011761:Pyrrolidonecarboxylic Acid; D012995:Solubility; D013912:Threonine / Q000378:metabolism*; D014357:Trypsin / Q000378:metabolism
D000596:Amino Acids; D002135:Calcium-Binding Proteins; D002241:Carbohydrates; D051847:Lithostathine; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; D010750:Phosphoproteins; D011760:Pyrrolidinones; C497617:REG1A protein, human; D013912:Threonine; D014357:Trypsin; D000116:Acetylgalactosamine; D011761:Pyrrolidonecarboxylic Acid; D000117:Acetylglucosamine