Production of a streptomycin-Park nucleotide complex by Streptomyces griseus.
A compound (compound X) with antibacterial activity was isolated from early-exponential-phase cultures of the streptomycin producer Streptomyces griseus and from protoplast cultures of the same strain. The protoplast cultures produced a larger amount of compound X than did the young hyphae. Both the mycelia and the protoplasts incorporated 14C-labeled myo-inositol, a precursor of streptomycin, into compound X, which has an amino acid content related to that of the cell wall peptidoglycan of the producer strain. Compound X may contain a streptomycin molecule covalently bound to a cell wall precursor unit, i.e., to a Park nucleotide (J. T. Park, J. Biol. Chem. 194:897-904, 1952), through the glutamic acid of the pentapeptide component.
Szabó
I
I
Institute of Biology, University Medical School, Debrecen, Hungary.
Penyige
A
A
Barabás
G
G
Szabó
G
G
Dinya
Z
Z
D000900:Anti-Bacterial Agents / Q000032:analysis / Q000096:biosynthesis*; D002473:Cell Wall / Q000032:analysis; D007553:Isotope Labeling; D015394:Molecular Structure; D009711:Nucleotides / Q000032:analysis / Q000096:biosynthesis*; D011523:Protoplasts / Q000378:metabolism; D013305:Streptomyces griseus / Q000378:metabolism*; D013307:Streptomycin / Q000096:biosynthesis*; D014538:Uridine Diphosphate N-Acetylmuramic Acid / Q000031:analogs & derivatives / Q000302:isolation & purification*; D014539:Uridine Diphosphate Sugars / Q000302:isolation & purification*
D000900:Anti-Bacterial Agents; D009711:Nucleotides; D014538:Uridine Diphosphate N-Acetylmuramic Acid; D014539:Uridine Diphosphate Sugars; C002225:UDP-N-acetylmuramic acid pentapeptide; D013307:Streptomycin