Role of transcriptional termination in the regulation of mu mRNA expression in B lymphocytes.
The synthesis of mRNA for mu-chain of membrane vs secretory IgM in normal resting B lymphocytes is stringently regulated. Very minimal amounts of microseconds mRNA are produced until the cells are stimulated. We have found a strong correlation between the initiation of mu S mRNA production and an alteration in the site of termination of polymerases transcribing the mu-gene. Furthermore, since the abundance of mu S mRNA is dependent on the differentiation stage of the B lymphocyte, we examined subpopulations of cells at various stages of activation and found that along with increased initiation of polymerases at the mu-locus, termination is moved further upstream in more highly differentiated cells. Therefore regulation of mu S mRNA may require factor(s) that induce transcriptional termination and these may be related to the factor(s) that augment transcriptional initiation.
Weiss
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Graduate Program in Immunology, University of Texas Southwestern Medical Center, Dallas 75235.
Michael
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Yuan
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D000818:Animals; D001402:B-Lymphocytes / Q000145:classification / Q000276:immunology / Q000378:metabolism*; D002469:Cell Separation; D005260:Female; D005786:Gene Expression Regulation; D007075:Immunoglobulin M / Q000378:metabolism; D007148:Immunoglobulin mu-Chains / Q000235:genetics* / Q000378:metabolism; D007958:Leukocyte Count; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D010443:Peptide Chain Termination, Translational; D010950:Plasma Cells / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism; D014158:Transcription, Genetic
D007075:Immunoglobulin M; D007148:Immunoglobulin mu-Chains; D012333:RNA, Messenger; D011947:Receptors, Antigen, B-Cell; C033027:secretory IgM