Enzyme reaction rate studies in electromotor neurons of the weakly electric fish Apteronotus leptorhynchus.
A histochemical analysis of reaction rates of a series of enzymes was performed in electromotor neurons of the weakly electric fish Apteronotus leptorhynchus. These neurons were selected because of their functional homogeneity. The high metabolic activity of these cells as well as their large size facilitate cytophotometric analysis in cryostat sections. Sections were incubated for the activity of hexokinase, glucose-6-phosphate dehydrogenase, succinate dehydrogenase, NADPH dehydrogenase, NADPH ferrihaemoprotein reductase and beta-hydroxybutyrate dehydrogenase. All media contained polyvinyl alcohol as tissue stabilizer and Nitro BT as final electron acceptor. Measurements were performed with a Vickers M85a cytophotometer. Linear relationships between the specific formation of formazan (test minus control reaction) and incubation time were obtained for all enzymes although some reactions showed an initial lag phase or an intercept with the ordinate. The relatively high activities of hexokinase, succinate dehydrogenase and the extremely low activity of hydroxybutyrate dehydrogenase indicate that energy is mainly supplied by glycolysis. Glucose-6-phosphate dehydrogenase showed a high activity whereas NADPH reductase and dehydrogenase activity were low in electromotor neurons, indicating that the NADPH generated is largely used for biosynthesis. Despite their synchronous firing pattern activity, electromotor neurons showed a considerable heterogeneity with respect to their metabolic activity.
Straatsburg
I H
IH
Laboratory of Cell Biology and Histology, University of Amsterdam, The Netherlands.
De Graaf
F
F
Van Noorden
C J
CJ
Van Raamsdonk
W
W
D000818:Animals; D004555:Electric Fish / Q000378:metabolism*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism; D006651:Histocytochemistry / Q000379:methods; D006884:Hydroxybutyrate Dehydrogenase / Q000378:metabolism; D008854:Microscopy, Electron; D009046:Motor Neurons / Q000166:cytology / Q000201:enzymology* / Q000648:ultrastructure; D016660:NAD(P)H Dehydrogenase (Quinone); D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D011808:Quinone Reductases / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000378:metabolism
D006884:Hydroxybutyrate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D013385:Succinate Dehydrogenase; D009251:NADPH-Ferrihemoprotein Reductase; D016660:NAD(P)H Dehydrogenase (Quinone); D011808:Quinone Reductases; D006593:Hexokinase