Alkali-induced revival of Bacillus spores after inactivation by glutaraldehyde.
Spores of Bacillus subtilis 168 were apparently fully inactivated by exposure to 2% (w/v) glutaraldehyde for 20 h but a few spores could be revived by further treatment with 10-100 mM NaOH. A similar effect was found with spores from a range of Bacillus species. A minimum concentration of 5% (w/v) glutaraldehyde was required to prevent the alkali-induced reactivation. The implications of these results for the use of glutaraldehyde as a sporicidal agent are discussed.
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School of Pure and Applied Biology, University of Wales College of Cardiff, U.K.
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Russell
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D000447:Aldehydes / Q000494:pharmacology*; D000468:Alkalies / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects*; D005976:Glutaral / Q000494:pharmacology*; D012972:Sodium Hydroxide / Q000494:pharmacology; D013171:Spores, Bacterial / Q000187:drug effects
D000447:Aldehydes; D000468:Alkalies; D012972:Sodium Hydroxide; D005976:Glutaral