title: Procoagulant activity of human neuroblastoma cell lines, in relation to cell growth, differentiation and cytogenetic abnormalities.
abstract: Procoagulant activity (PCA) was investigated in relation to cell growth, differentiation, and cytogenetics in seven human neuroblastoma cell lines. Before 5-bromodeoxyuridine (BrdUrd) treatment, PCA was notably heterogeneous, with the highest activity in NCG (S-type in morphology) 40- to 100-fold greater than the lowest activity in SK-N-DZ (N-type). PCA was not related to 1p abnormalities. After BrdUrd treatment at 5 micrograms/ml for 6 days, PCA increased 6.8-fold in GOTO and 2.7-fold in SK-N-DZ with associated growth inhibition and morphological changes (I-type morphology converted to S-type in GOTO and N-type converted to an advanced N-type in SK-N-DZ). In contrast, only growth suppression was observed in 2 other cell lines, and no changes in PCA, growth or morphology were induced in the remaining 3 cell lines.
authors.affiliation: Department of Pediatrics, Kyoto Prefectural University of Medicine.
authors.forename: N
authors.initials: N
authors.lastname: Esumi
authors.forename: S
authors.initials: S
authors.lastname: Imashuku
authors.forename: K
authors.initials: K
authors.lastname: Tsunamoto
authors.forename: S
authors.initials: S
authors.lastname: Todo
authors.forename: S
authors.initials: S
authors.lastname: Misawa
authors.forename: T
authors.initials: T
authors.lastname: Goto
authors.forename: Y
authors.initials: Y
authors.lastname: Fujisawa
mesh_terms: D001779:Blood Coagulation Factors / Q000032:analysis*; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002869:Chromosome Aberrations; D003546:Cysteine Endopeptidases / Q000032:analysis*; D006801:Humans; D009363:Neoplasm Proteins; D009447:Neuroblastoma / Q000032:analysis* / Q000235:genetics / Q000473:pathology; D014407:Tumor Cells, Cultured
chemical_list: D001779:Blood Coagulation Factors; D009363:Neoplasm Proteins; D003546:Cysteine Endopeptidases; C030910:cancer procoagulant; D001973:Bromodeoxyuridine