Circulating mononuclear cells from pure red cell aplasia of chronic lymphocytic leukemia suppress in vitro erythropoiesis.
In vitro studies were performed in a patient with B-cell chronic lymphocytic leukemia who developed pure red cell aplasia (CLL-PRCA). The patient's irradiated circulating mononuclear blood cells and supernatant markedly inhibited normal marrow erythroid (but not granulocyte-monocyte) progenitor colony proliferation. In contrast, irradiated peripheral blood mononuclear cells and supernatant obtained from a B-CLL patient (Rai stage III) and from a hematologically normal donor, did not affect hematopoietic progenitor colony growth. These findings suggest that the anemia of CLL-PRCA evolves different mechanisms of those causing anemia in CLL, and is mediated through cellular and secretory mechanisms.
Estrov
Z
Z
Department of Medicine B, Kaplan Hospital, Rehovot, Israel.
Berrebi
A
A
Kusminsky
G
G
Resnitzky
P
P
D000368:Aged; D001853:Bone Marrow / Q000473:pathology; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay / Q000379:methods; D004920:Erythropoiesis; D005260:Female; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000097:blood* / Q000473:pathology; D007963:Leukocytes, Mononuclear / Q000473:pathology* / Q000528:radiation effects; D008198:Lymph Nodes / Q000473:pathology; D012010:Red-Cell Aplasia, Pure / Q000097:blood* / Q000473:pathology