title: [Application of a method of material-energy balance for controlling the process of microbiological synthesis of riboxin].
abstract: Possible control of riboxin biosynthesis on the basis of the information provided with the method of material-energy balance was studied. Correspondence of the cultivation conditions to the directed biosynthesis was established by comparing the experimental values of the respiration coefficient delta CO2/delta O2, the coefficient of constructive and energy metabolism conjugate Cc/e and the ratio of the consumed amounts of glucose and ammonium nitrogen delta S/delta NH4+ with the theoretical values of these parameters. The control principle included changing of the process operation parameters for providing cultivation conditions approximating the experimental values of Cc/e, delta CO2/delta O2 and delta S/delta NH4+ to their theoretical values. It was shown possible to control directed aerobic growth of the culture by changing the aeration conditions. A decrease in the initial aeration rate promoted more rapid transition of the microbial population to the exponential growth phase and provided for a certain period cultivation conditions corresponding to the conditions of conjugate metabolism. It was found that the respiration coefficient could be used not only as a parameter reflecting one of the forms of the cell physiological activity manifestation but also as an optimization criterion in development of a controlled cultivation process.
authors.forename: A I
authors.initials: AI
authors.lastname: Toĭtman
authors.forename: M A
authors.initials: MA
authors.lastname: Malkov
authors.forename: G I
authors.initials: GI
authors.lastname: Kil'fin
mesh_terms: D001412:Bacillus subtilis / Q000378:metabolism*; D001431:Bacteriological Techniques; D004734:Energy Metabolism; D007290:Inosine Diphosphate / Q000096:biosynthesis*; D007292:Inosine Nucleotides / Q000096:biosynthesis*
chemical_list: D007292:Inosine Nucleotides; D007290:Inosine Diphosphate
vernacular_title: Primenenie metoda material'no-énergeticheskogo balansa dlia upravleniia protsessom mikrobiologicheskogo sinteza riboksina.