Modulation by IFN-gamma of the metastatic ability of murine, human, and H-2-transfected tumor cells.
Interferon-gamma (IFN-gamma) can enhance the experimental metastatic ability of B16 melanoma. The in vitro treatment with IFN-gamma of four clones derived from the murine mammary adenocarcinoma TS/A increased the number of lung colonies observed after intravenous injection in syngeneic mice. The spontaneous metastatic ability of these clones was not altered by the IFN-gamma pretreatment nor by daily intratumor injection of low-dose IFN-gamma. The experimental metastatic ability in nude mice of the human rhabdomyosarcoma cell line RD was decreased by in vitro pretreatment with IFN-gamma. To study the role played by major histocompatibility complex gene products in the IFN-gamma-mediated enhancement of B16 experimental metastasis, a mutant B16 clone, B78H1, was transfected with the H-2Kb gene. B78H1 cells are not capable of expressing H-2b even after treatment with IFN-gamma; IFN-gamma readily induced high levels of H-2Kb in a set of transfected clones, but did not enhance their experimental metastatic ability.
Lollini
P L
PL
Istituto di Cancerologia, Università di Bologna, Italy.
De Giovanni
C
C
Nicoletti
G
G
Scotlandi
K
K
Landuzzi
L
L
Nanni
P
P
D000818:Animals; D006183:H-2 Antigens / Q000235:genetics*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D015674:Mammary Neoplasms, Animal / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009362:Neoplasm Metastasis; D011994:Recombinant Proteins; D012208:Rhabdomyosarcoma / Q000473:pathology; D014162:Transfection; D014407:Tumor Cells, Cultured
D006183:H-2 Antigens; D011994:Recombinant Proteins; D007371:Interferon-gamma