A radiometric microbiological assay for pantothenic acid in biological fluids.
A semi-automated radiometric-microbiological assay (RMA) was developed and applied for the analysis of pantothenic acid in human blood and milk. The RMA is based on the measurement of 14CO2 produced from the metabolism of L-[1-14C]methionine or L-[1-14C]valine by the yeast Kloeckera brevis in the presence of pantothenic acid. The assay was shown to be sensitive and specific for the analysis of this vitamin in biological fluids.
Guilarte
T R
TR
Department of Environmental Health Sciences, Johns Hopkins University, School of Hygiene and Public Health, Baltimore, Maryland 21205.
D001681:Biological Assay / Q000379:methods*; D002245:Carbon Dioxide / Q000032:analysis; D006801:Humans; D008715:Methionine / Q000378:metabolism; D008895:Milk, Human / Q000032:analysis*; D010205:Pantothenic Acid / Q000097:blood*; D011869:Radioligand Assay; D014633:Valine / Q000378:metabolism; D015003:Yeasts / Q000378:metabolism
D002245:Carbon Dioxide; D010205:Pantothenic Acid; D008715:Methionine; D014633:Valine