Phagosome-lysosome fusions in macrophages infected by Mycobacterium avium: role of mycosides-C and other cells surface components.
The phagosome-lysosome fusions (PLE) were assessed in case of bone-marrow macrophages infected by the opportunistic species Mycobacterium avium, employing the acid-phosphatase (AcPase) electron-cytochemistry. The role of surface components was evaluated by coating the bacteria prior to phagocytosis by specific M. avium antiserum or the anti-mycosides-C serum raised in rabbit. PLF was evaluated under the electron microscope during (2, 4 hours), or after (24 hours) phagocytosis. The preliminary results suggest that although M. avium surface components intervene in PLF inhibition, the role of mycosides-C among these surface components (effectively intervening in PLF inhibition) is questionable.
Fréhel
C
C
Unité de Microscopie Electronique, Institut Pasteur, France.
Rastogi
N
N
D000818:Animals; D000942:Antigens, Bacterial; D002478:Cells, Cultured; D006017:Glycolipids / Q000276:immunology; D006020:Glycopeptides / Q000276:immunology; D008247:Lysosomes / Q000648:ultrastructure; D008264:Macrophages / Q000276:immunology / Q000382:microbiology* / Q000648:ultrastructure; D008561:Membrane Fusion; D009162:Mycobacterium avium / Q000254:growth & development* / Q000276:immunology / Q000472:pathogenicity; D010588:Phagosomes / Q000648:ultrastructure
D000942:Antigens, Bacterial; D006017:Glycolipids; D006020:Glycopeptides; C010762:peptidoglycolipids