A simple in vitro test to evaluate biocompatibility of dialysis membranes.
As arachidonic acid metabolites are implicated in hypersensitivity reactions, we measured arachidonic acid metabolites of dialysed patient's granulocytes, preincubated with different dialysis membranes. Results indicate that cuprophan and cellulose acetate membranes partially inhibit in vitro production of 15-HETE and 5-HETE, whereas polyacrylonitrile membrane does not. This suggests that polyacrylonitrile is a more biocompatible membrane.
Vincent
D
D
CHU Dupuytren, Limoges, France.
Charmes
J P
JP
Benzakour
M
M
Gualde
N
N
Rigaud
M
M
Leroux-Robert
C
C
D000180:Acrylic Resins; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000097:blood; D001672:Biocompatible Materials; D002482:Cellulose / Q000031:analogs & derivatives; D006801:Humans; D007688:Kidneys, Artificial / Q000009:adverse effects; D007962:Leukocytes / Q000378:metabolism; D008422:Materials Testing / Q000379:methods*; D008567:Membranes, Artificial
D000180:Acrylic Resins; D001095:Arachidonic Acids; D001672:Biocompatible Materials; D008567:Membranes, Artificial; C010504:polyacrylonitrile; D016718:Arachidonic Acid; C005062:acetylcellulose; D002482:Cellulose; C009865:cuprammonium cellulose