Eicosanoids produced during interactions between Pseudomonas aeruginosa and alveolar macrophages are species-dependent.
Eicosanoid production during phagocytosis of pyogenic bacteria by rabbit alveolar macrophages was studied as a model of early events in the pathogenesis of pneumonia. Adherent alveolar macrophages, prelabelled with [3H]-arachidonic acid (AA), were incubated with live, opsonized Staphylococcus aureus or Pseudomonas aeruginosa (bacteria:macrophage ratio of 50:1) at 37 degrees C for 90 min. Supernatant eicosanoids were extracted and separated by reverse phase high performance liquid chromatography (RP-HPLC). While the amounts of labelled PGE2, TXB2, and PGD2 produced in response to the two organisms were equal, the amount of PGF2 alpha elicited by S. aureus amounted to three times that released during macrophage challenge with P. aeruginosa. Overall, preferential release of cyclooxygenase products occurred during phagocytosis of S. aureus. In contrast, eicosanoids identified presumptively as oxygenated metabolites of AA predominated in cultures challenged with opsonized P. aeruginosa. Live, non-opsonized P. aeruginosa elicited the same profile of eicosanoids, but in reduced amounts. Inhibitor studies indicated that these AA derivatives were not synthesized via the macrophage lipoxygenase pathway. Their production was dependent on the viability of P. aeruginosa. Macrophages challenged with opsonized, heat-killed P. aeruginosa resulted in production of an eicosanoid profile similar to that elicited by S. aureus. Secondary metabolism by P. aeruginosa of eicosanoids released from the macrophage did not contribute to the unique profile produced during the interaction of this organism with labelled macrophages. Our data indicate that during binding to macrophages, the primary human pathogen, P. aeruginosa, specifically modulates the profile of eicosanoids produced. This effect on inflammatory mediators may be of biological significance in the pathogenesis of pneumonia.
Sorrell
T C
TC
University of Sydney Department of Medicine, NSW, Australia.
Rochester
C P
CP
Breen
F N
FN
Müller
M
M
D000818:Animals; D005260:Female; D008264:Macrophages / Q000276:immunology*; D010587:Phagocytosis; D011018:Pneumonia, Pneumococcal; D011451:Prostaglandin-Endoperoxide Synthases / Q000032:analysis; D011453:Prostaglandins / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000276:immunology* / Q000378:metabolism; D011817:Rabbits; D013045:Species Specificity; D013211:Staphylococcus aureus / Q000378:metabolism; D013929:Thromboxane B2 / Q000378:metabolism
D011453:Prostaglandins; D013929:Thromboxane B2; D011451:Prostaglandin-Endoperoxide Synthases