Dynamics of glutathione levels in liver and indicatory enzymes in serum in acetaminophen intoxication in mice.
Acetaminophen (AA) was administered i.p. to Swiss mice as a single dose 100, 200, 300, 400 and 600 mg/kg. At different time periods after administration, the mice were sacrificed. Serum glutamate-pyruvate transaminase (SGPT) and sorbitol dehydrogenase (SDH) as well as glutathione (GSH) levels in the liver were determined. It was found that the effective dose ranged within 200-600 mg/kg. Changes in GSH level occurred shortly after acetaminophen administration, whereas changes in the activity of indicatory enzymes were slightly delayed compared to this process. Conditions allowing for parallel observations of all three indices under investigation occurred 4 hrs after acetaminophen administration. With regard to glutathione, directly measured decrease, as compared to control levels, may be used as the yardstick of the changes. Changes in the activity of indicatory enzymes may be better expressed in the dose-response arrangement. For all the indices determined 4 hrs after acetaminophen administration, ED50 is in the range 200-300 mg/kg.
Brzeźnicka
E A
EA
Piotrowski
J K
JK
D000082:Acetaminophen / Q000008:administration & dosage / Q000633:toxicity*; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D005978:Glutathione / Q000032:analysis*; D007064:L-Iditol 2-Dehydrogenase / Q000097:blood; D008099:Liver / Q000737:chemistry* / Q000201:enzymology; D008297:Male; D051379:Mice
D000082:Acetaminophen; D007064:L-Iditol 2-Dehydrogenase; D000410:Alanine Transaminase; D005978:Glutathione