Isolation, characterization and quantification of apolipoproteins A-1 and B of the Golden Syrian hamster (Mesocricetus auratus) and modification of their levels by dietary cholesterol.
1. Apolipoprotein A-1, isolated from hamster high density lipoprotein, possessed a molecular weight of approximately 27,000. 2. Its amino acid composition differed from human apo A-1 and it contained a higher threonine to serine ratio and a higher methionine and leucine content. 3. The concentration in normal serum was 126.0 +/- 1.9 mg/dl. 4. Apolipoprotein B, isolated from hamster low density lipoprotein consisted of three major components when analyzed by SDS-polyacrylamide gel electrophoresis with Mrs of 635 Kd, 460 Kd and 305 Kd respectively. 5. Hamster apo B possessed a higher aspartic acid to glutamic acid ratio and a higher methionine and valine content than human apo B. 6. The concentration in normal serum was 20.9 +/- 1.0 mg/dl. 7. The apolipoprotein and lipoprotein profile of hamsters fed a high cholesterol diet for 30 days changed considerably. 8. Total serum cholesterol levels increased 7 fold; LDL levels increased 14 fold; HDL levels doubled and total serum triglyceride increased 3 fold. 9. Apo A-1 levels increased by 45% and apo B levels increased 5 fold.
Burton
P M
PM
Department of Cancer and Developmental Biology, Syntex Research, Palo Alto, CA 94304.
Chiou
Y M
YM
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D016632:Apolipoprotein A-I; D001054:Apolipoproteins A / Q000032:analysis / Q000302:isolation & purification*; D001055:Apolipoproteins B / Q000032:analysis / Q000302:isolation & purification*; D002791:Cholesterol, Dietary / Q000494:pharmacology*; D006224:Cricetinae / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D007118:Immunoassay; D008075:Lipoproteins, HDL / Q000032:analysis / Q000302:isolation & purification*; D008297:Male; D008647:Mesocricetus / Q000378:metabolism*; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis
D000596:Amino Acids; D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D001055:Apolipoproteins B; D002791:Cholesterol, Dietary; D008075:Lipoproteins, HDL; D011506:Proteins