Recombinant peptides derived from the env-gene of HIV-2 in the serodiagnosis of HIV-2 infections.
We produced recombinant envelope-derived peptides of HIV-2 for use in a diagnostic enzyme-linked immunosorbent assay (ELISA) or Western blot by expressing several restriction enzyme fragments from the env gene of HIV-2 in the bacterial fusion vector pEX-3. On Western blots, 17 out of 18 anti-HIV-2-positive sera available to us reacted strongly with those recombinant peptides which were derived from, or extended into, the transmembrane protein of HIV-2. In contrast, recombinant peptides derived from the external envelope glycoprotein were only weakly recognized by two sera. We observed a cross-reactivity of some human sera containing antibodies to HIV-1 with the HIV-2 peptides derived from the transmembrane protein of HIV-2. In spite of this cross-reactivity, a serological distinction between anti-sera to HIV-1 and HIV-2 can be attempted by simultaneous testing in ELISA on recombinant peptides derived from the transmembrane protein of HIV-1 and HIV-2.
Schulz
T F
TF
Boltzmann Institute for AIDS Research, Innsbruck, Austria.
Oberhuber
W
W
Hofbauer
J M
JM
Hengster
P
P
Larcher
C
C
Gürtler
L C
LC
Tedder
R
R
Wachter
H
H
Dierich
M P
MP
D015492:AIDS Serodiagnosis; D015153:Blotting, Western; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D015483:HIV Antibodies / Q000032:analysis; D015498:HIV-2 / Q000235:genetics / Q000276:immunology*; D010957:Plasmids; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D014760:Viral Fusion Proteins / Q000032:analysis
D015483:HIV Antibodies; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins