Rapid method for identifying subclones bridging gaps between contiguous DNA sequences.
Randomly sequencing of a limited number of unidirectional deletion subclones efficiently generated contiguous DNA sequences with some gaps between them. Gap-bridging subclones were identified by agarose gel electrophoresis. Those located at either end of each individual contiguous sequence were used as standard marker molecules; the subclones between them proved to be correctly identified in most cases. A 4064 bp DNA fragment containing the psr gene was sequenced with the strategy described in this paper.
Lu
Y Y
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Shanghai Institute of Biochemistry, Academia sinica.
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Hong
G F
GF
D001483:Base Sequence; D002872:Chromosome Deletion; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA; D004587:Electrophoresis, Agar Gel
D004247:DNA