Ia-expressing microglial cells in experimental allergic encephalomyelitis in rats.
Monoclonal antibodies (MRC OX-6 and OX-17) recognized three types of cells expressing Ia antigen during the course of acute experimental allergic encephalomyelitis (EAE) in rats. In earlier stages of the disease, in animals with or without paralysis, Ia antigens were mostly localized to subarachnoidal and perivascular lymphocytic and histiocytic cell infiltrates, possibly serving as antigen-presenting cells. On the other hand, in convalescent rats, Ia antigens were expressed in a large number of cells with dendritic processes heavily populating the spinal gray matter. The appearance of these Ia-expressing cells in the convalescent stage coincided with the development of degenerating axon terminals in the spinal gray matter. These Ia-expressing cells possessed morphological features characteristic of microglia and were positive for ML-1 lectin but did not express glial fibrillary acidic protein. Immune electron microscopy disclosed the presence of Ia reaction products in the Golgi apparatus, endoplasmic reticulum and plasma membrane of these cells with dendritic processes, indicating active synthesis of Ia molecules in microglia. In addition, Ia antigens were localized to the cells with ultrastructural features of macrophages. Thus, Ia-expressing cells in EAE seems to play dual roles: the induction of immunological reactions during earlier stages and the participation in reparative processes during convalescence.
Konno
H
H
Department of Neurological Sciences, Tohoku University School of Medicine, Sendai, Japan.
Yamamoto
T
T
Iwasaki
Y
Y
Saitoh
T
T
Suzuki
H
H
Terunuma
H
H
D000818:Animals; D000911:Antibodies, Monoclonal; D001921:Brain / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology; D007143:Immunoglobulin Heavy Chains / Q000378:metabolism*; D007137:Immunoglobulin alpha-Chains / Q000378:metabolism*; D008297:Male; D009457:Neuroglia / Q000276:immunology* / Q000648:ultrastructure; D051381:Rats; D011917:Rats, Inbred Lew; D013116:Spinal Cord / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology
D000911:Antibodies, Monoclonal; D007143:Immunoglobulin Heavy Chains; D007137:Immunoglobulin alpha-Chains