Oleate-induced translocation of protein kinase C to hepatic microsomal membranes.
The incubation of rat liver homogenates in the presence of oleate induces the translocation of protein kinase C from the cytosol to the endoplasmic reticulum membranes. The half-maximal effect was obtained at 0.3 mM oleate. The redistribution of this enzyme induced by oleate was also obtained with purified protein kinase C and hepatic microsomal membranes. This effect seems to be mediated by long-chain fatty acids since translocation was not obtained with esterified derivatives.
Boscá
L
L
Instituto de Bioquimica, Facultad de Farmacia, Madrid, Spain.
Díaz-Guerra
M J
MJ
Mojena
M
M
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D001921:Brain / Q000201:enzymology; D002118:Calcium / Q000494:pharmacology; D005227:Fatty Acids / Q000494:pharmacology; D007425:Intracellular Membranes / Q000201:enzymology; D007527:Isoenzymes / Q000378:metabolism; D007546:Isoquinolines / Q000494:pharmacology; D007976:Leupeptins / Q000494:pharmacology; D008862:Microsomes, Liver / Q000201:enzymology*; D019301:Oleic Acid; D009829:Oleic Acids / Q000494:pharmacology*; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology; D010711:Phosphatidate Phosphatase / Q000378:metabolism; D010879:Piperazines / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D013096:Spermine / Q000494:pharmacology
D001095:Arachidonic Acids; D005227:Fatty Acids; D007527:Isoenzymes; D007546:Isoquinolines; D007976:Leupeptins; D009829:Oleic Acids; D010879:Piperazines; D016718:Arachidonic Acid; D013096:Spermine; D019301:Oleic Acid; D015240:Phorbol 12,13-Dibutyrate; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D011493:Protein Kinase C; D010711:Phosphatidate Phosphatase; C032854:leupeptin; D002118:Calcium