[Experimental study on renal protection against damage in kidneys subjected warm ischemia--protective effect of FOY, SOD, and PEG-SOD on ischemic acute renal failure].
Oxygen free radicals have been implicated in the pathogenesis of tissue injury consequent to ischemia/reperfusion in several different organs, including heart, bowel, and kidney. In this study, the protective effect of FOY, SOD, and PEG-SOD against kidney damage resulting from warm ischemia and reperfusion was investigated in the rat. FOY (Gabexate mesilate), one of protease inhibitor, has been suggested to inhibit the activity of superoxide in polymorphonuclear leucocyte in recent reports. PEG-SOD (polyethyleneglycol-modified SOD), recently synthesized on the basis of SOD, has an additional value in comparison with SOD. WKA rats underwent right nephrectomy, and occlusion of the left renal artery, vein, and ureter for 60 minutes. FOY (50mg/kg, DIV.) was administrated from 5 minutes before reperfusion to 5 minutes after reperfusion to the rat. SOD (2mg/kg, 5mg/kg, 10mg/kg, IV.) and PEG-SOD (2mg/kg, 5mg/kg, IV.) were administrated at 10 minutes before reperfusion. 48 hours after operation, the measurement of urine output (60 minutes) was made, and BUN, Cr, K, UUN, UCr were measured at this point. Creatinine clearance was calculated from these results. The left kidney was removed and histological examination was performed. Serum BUN, Cr level were greatly elevated, and creatinine was diminished in the group of ischemic untreated rats (n = 8). In the groups of rats treated with FOY (n = 9), SOD (5mg/kg, 10mg/kg; n = 5, respectively), and PEG-SOD (2mg/kg, 5mg/kg; n = 5, respectively), serum Cr was significantly lower and creatinine clearance was significantly higher than control untreated group. Furthermore, tubular injury was less in histological examination in these groups.(ABSTRACT TRUNCATED AT 250 WORDS)
Sasaki
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Second Department of Surgery, Hokkaido University School of Medicine, Sapporo, Japan.
D058186:Acute Kidney Injury / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D000818:Animals; D016670:Gabexate; D006146:Guanidines / Q000627:therapeutic use*; D007511:Ischemia / Q000150:complications*; D007668:Kidney / Q000098:blood supply*; D007684:Kidney Tubules / Q000473:pathology; D009336:Necrosis; D011092:Polyethylene Glycols / Q000627:therapeutic use; D051381:Rats; D011919:Rats, Inbred Strains; D015427:Reperfusion Injury / Q000150:complications*; D013482:Superoxide Dismutase / Q000627:therapeutic use*
D006146:Guanidines; D011092:Polyethylene Glycols; D016670:Gabexate; D013482:Superoxide Dismutase; C063471:polyethylene glycol-superoxide dismutase