Actin-associated proteins and fibronectin in the fetal human inner ear.
The distribution of alpha-actinin, vinculin, alpha-spectrin, beta-spectrin and fibronectin was analyzed in 14- to 21-week-old fetal human inner ears using immunofluorescence microscopy. Staining for alpha-actinin was fairly evenly distributed at the epithelial surfaces of all five vestibular organs, whereas in the cochlea it was mainly at the surface of the receding greater epithelial ridge and in some foci apically at the lesser epithelial ridge. Fluorescence for vinculin was observed mainly at the surface of vestibular organs, but was lacking in the LER. Intense fluorescence for alpha-spectrin was found at the apical surface of individual cells of the cristae and maculae. Antibodies against beta-spectrin mainly stained the endothelial cells of blood vessels, but faint staining of the epithelial cell surfaces of the vestibular organs was also detected. The fluorescence pattern of the actin-associated proteins is indicative of structural differences between cochlear and vestibular hair cells. Fibronectin was identified only between mesenchymal cells and its functional importance in the mature inner ear epithelia can be discounted.
Anniko
M
M
Department of Oto-Rhino-Laryngology and Head & Neck Surgery, University Hospital, Umeå, Sweden.
Thornell
L E
LE
Virtanen
I
I
D000199:Actins / Q000032:analysis*; D007758:Ear, Inner / Q000378:metabolism*; D005333:Fetus; D005353:Fibronectins / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006801:Humans; D009124:Muscle Proteins / Q000032:analysis*; D013049:Spectrin / Q000032:analysis*; D016596:Vinculin
D000199:Actins; D005353:Fibronectins; D009124:Muscle Proteins; D016596:Vinculin; D013049:Spectrin