Influences of season and artificial photoperiod on stallions: pituitary and testicular responses to exogenous GnRH.
Effects of season and photoperiod on the anterior pituitary gland and testes were studied by responses to exogenous GnRH. Stallions were assigned to one of three treatments: 1) control, exposed to natural day length; 2) S-L, 8 h of light and 16 h dark (8:16) for 20 wk beginning July 16, 1982 then 16:8 from December 2, 1982 until March 5, 1984; or 3) S-S, 8:16 from July 16, 1982 until March 5, 1984. Approximately every 8 wk, stallions were administered GnRH (2 micrograms/kg BW) and blood was sampled at 20-min intervals for 2 h before and 8 h after GnRH administration. Concentrations of LH, FSH and testosterone were determined. Baseline concentrations (mean of pre-GnRH samples) of all hormones fluctuated seasonally (P less than .05), but only LH and testosterone displayed seasonal changes (P less than .05) in maximum response to GnRH (highest concentration above baseline after GnRH). The FSH response to GnRH was not affected (P greater than .05) by season, photoperiod or the season X treatment interaction. Exposure of S-L stallions to 16:8 in December resulted in early recrudescence of baseline concentrations of LH, FSH and testosterone. Maximum concentration of testosterone in response to GnRH was stimulated by 16:8, but the increase in baseline LH concentrations in S-L stallions was not associated with an increase in maximum LH response to GnRH. Seasonal patterns of baseline concentrations of FSH and testosterone and maximum LH response to GnRH in S-S stallions were similar to those for control stallions.(ABSTRACT TRUNCATED AT 250 WORDS)
Clay
C M
CM
Anim. Reprod. Lab., Colorado State Univ., Fort Collins 80523.
Squires
E L
EL
Amann
R P
RP
Nett
T M
TM
D000818:Animals; D005640:Follicle Stimulating Hormone / Q000097:blood / Q000378:metabolism; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D006736:Horses / Q000502:physiology*; D008027:Light; D007986:Luteinizing Hormone / Q000097:blood / Q000378:metabolism; D008297:Male; D010507:Periodicity; D010903:Pituitary Gland, Anterior / Q000187:drug effects* / Q000378:metabolism; D012621:Seasons; D013737:Testis / Q000187:drug effects* / Q000378:metabolism; D013739:Testosterone / Q000097:blood / Q000378:metabolism
D007987:Gonadotropin-Releasing Hormone; D013739:Testosterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone