title: A locus involved in kanamycin, chloramphenicol and L-serine resistance is located in the bglY-galU region of the Escherichia coli K12 chromosome.
abstract: Spontaneous mutants of Escherichia coli K12 displaying an increased level of the kanamycin resistance conferred by plasmid pGR71 were selected. Several mutants obtained in this way apparently carry large chromosomal deletions extending into galU and/or bglY (27 min). This positive selection of deletions allowed detection of a new locus located between galU and bglY. Deletions of this locus are responsible for increased resistance to kanamycin (Irk), decreased resistance to L-serine in minimal medium (Drs) and decreased resistance to chloramphenicol (Drc) when a cat gene is present in the bacteria.
authors.affiliation: Laboratoire de Génétique Microbienne, Université de Louvain, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: Lejeune
authors.forename: P
authors.initials: P
authors.lastname: Bertin
authors.forename: C
authors.initials: C
authors.lastname: Walon
authors.forename: K
authors.initials: K
authors.lastname: Willemot
authors.forename: C
authors.initials: C
authors.lastname: Colson
authors.forename: A
authors.initials: A
authors.lastname: Danchin
mesh_terms: D002702:Chloramphenicol Resistance / Q000235:genetics*; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D005838:Genotype; D007613:Kanamycin Resistance / Q000235:genetics*; D009154:Mutation; D010641:Phenotype; D010957:Plasmids; D012694:Serine / Q000494:pharmacology*
chemical_list: D012694:Serine