Modulation of experimental autoimmune uveoretinitis by adoptive transfer of cells from rats immunized with anti-S-antigen monoclonal antibody.
Preimmunization of rnu/+ rats with the mouse monoclonal antibody S2D2 against retinal S-antigen (S-Ag) leads to an anti-idiotypic (anti-Id) response and to protection against experimental autoimmune uveoretinitis (EAU) provoked by a subsequent challenge with S-Ag in complete Freund's adjuvant (CFA). Suppression of EAU can be passively transferred using lymph node and/or spleen cells from donors with active anti-Id immunity to naive rnu/+ recipients, prior to immunization with bovine S-Ag in CFA. In contrast, passive administration of IgG from S2D2-immunized rats did not produce suppression. High levels of anti-Id S2D2 antibodies were found: (1) in rats presenting an inhibited EAU after sensitization with S2D2 or after passive transfer of anti-S2D2 cells prior to the S-Ag immunization; and (2) in rats with a severe EAU after transfer of anti-S2D2 IgG associated with anti-S2D2 cells prior to the S-Ag challenge. No effect on the anti-S-Ag antibody level could be detected. These experiments suggest that EAU, a mostly T cell-dependent disease, can be down regulated by anti-Id lymphoid cells.
de Kozak
Y
Y
Unité de Recherche d'Ophtalmologie, INSERM U 86-CNRS, Institut Biomédical des Cordeliers, Paris, France.
Mirshahi
M
M
Boucheix
C
C
Faure
J P
JP
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis; D000911:Antibodies, Monoclonal / Q000008:administration & dosage*; D000941:Antigens / Q000276:immunology*; D019393:Arrestin; D001327:Autoimmune Diseases / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D005136:Eye Proteins / Q000276:immunology*; D007114:Immunization; D007116:Immunization, Passive; D007074:Immunoglobulin G / Q000008:administration & dosage; D016219:Immunotherapy, Adoptive; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D011922:Rats, Mutant Strains; D012173:Retinitis / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000941:Antigens; D019393:Arrestin; D005136:Eye Proteins; D007074:Immunoglobulin G