Induction of blood coagulation factor VIII by sodium butyrate in Balb/c 3T3 cells.
Balb/c 3T3 cells have long been used in cell biological studies. When cells were treated with 2 mM sodium butyrate they became flattened and their nuclei became small relative to the cytoplasm. Electron micrographically, intracellular organellae developed significantly: the number of mitochondria increased and membranous structures, namely the rough-surfaced endoplasmic reticulum and Golgi apparatus, developed markedly. Blood coagulation factor VIII was induced in the presence of 2 mM sodium butyrate in a cell sonicate, but not in culture medium. The amount of this factor was almost 100-fold that in the nontreated cells 4 days after culture initiation, indicating that the cloned Balb/c 3T3 cells were of endothelial origin.
Matsuhisa
T
T
Department of Cell Biology, Center for Adult Diseases, Osaka, Japan.
Okada
M
M
Mori
Y
Y
D000818:Animals; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D002452:Cell Count / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure; D002999:Clone Cells; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000166:cytology*; D005169:Factor VIII / Q000096:biosynthesis*; D005227:Fatty Acids / Q000494:pharmacology; D007700:Kinetics; D008854:Microscopy, Electron; D015388:Organelles / Q000648:ultrastructure
D002087:Butyrates; D005227:Fatty Acids; D020148:Butyric Acid; D005169:Factor VIII; D004121:Dimethyl Sulfoxide