title: Cloning and sequencing of the peroxisomal amine oxidase gene from Hansenula polymorpha.
abstract: We have cloned the AMO gene, encoding the microbody matrix enzyme amine oxidase (EC 1.4.3.6) from the yeast Hansenula polymorpha. The gene was isolated by differential screening of a cDNA library, immunoselection, and subsequent screening of a H. polymorpha genomic library. The nucleotide sequence of a 3.6 kilobase stretch of DNA containing the amine oxidase (AMO) gene was determined. The AMO gene contains an open reading frame of 692 amino acids, with a relative molecular mass of 77,435. The 5' and 3' ends of the gene were mapped and show that the transcribed region measures 2134 nucleotides. The derived amino-acid sequence was confirmed by sequencing an internal proteolytic fragment of the purified protein. Amine oxidase contains the tripeptide sequence Ser-Arg-Leu, located 9 residues from the carboxy terminus, which may represent the topogenic signal for protein import into microbodies.
authors.affiliation: Laboratory of Biochemistry, Groningen University, The Netherlands.
authors.forename: P G
authors.initials: PG
authors.lastname: Bruinenberg
authors.forename: M
authors.initials: M
authors.lastname: Evers
authors.forename: H R
authors.initials: HR
authors.lastname: Waterham
authors.forename: J
authors.initials: J
authors.lastname: Kuipers
authors.forename: A C
authors.initials: AC
authors.lastname: Arnberg
authors.forename: G
authors.initials: G
authors.lastname: AB
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000235:genetics*; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D003062:Codon; D004271:DNA, Fungal / Q000648:ultrastructure; D005800:Genes, Fungal; D008830:Microbodies / Q000201:enzymology; D008969:Molecular Sequence Data; D010843:Pichia / Q000235:genetics* / Q000648:ultrastructure; D021382:Protein Sorting Signals / Q000235:genetics; D004718:Saccharomycetales / Q000235:genetics*
chemical_list: D000596:Amino Acids; D003062:Codon; D004271:DNA, Fungal; D021382:Protein Sorting Signals; D006631:Amine Oxidase (Copper-Containing)