Enumeration of single IFN-gamma-producing cells in mice during viral and bacterial infection.
A solid phase immunoenzymatic technique was employed for detecting single IFN-gamma-producing cells (IFN-gamma PC) in the mouse. After infection with lymphocytic choriomeningitis virus or Listeria monocytogenes, the numbers of IFN-gamma PC in spleens began to rise on day 4, attained maxima on days 7 and 8, and declined thereafter. Negative selection in vitro by use of mAb and C allowed phenotypic identification of the producer cells; most, if not all, carried Thy-1, and approximately one half expressed CD4, the other half, CD8. Depletion of cells in vivo by treatment of mice with mAb led to somewhat different results; again, anti-Thy-1 antibody eliminated essentially all IFN-gamma PC, but considerably more than 50% were either CD4+ or CD8+, suggesting regulatory interactions between these T lymphocyte subsets with regard to generation of the lymphokine.
Gessner
A
A
Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Universität Hamburg, Germany.
Moskophidis
D
D
Lehmann-Grube
F
F
D000818:Animals; D002469:Cell Separation; D005260:Female; D007124:Immunoenzyme Techniques; D007371:Interferon-gamma / Q000096:biosynthesis*; D007700:Kinetics; D007958:Leukocyte Count; D008088:Listeriosis / Q000276:immunology / Q000378:metabolism*; D008216:Lymphocytic Choriomeningitis / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D010641:Phenotype; D013154:Spleen; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology / Q000378:metabolism*
D007371:Interferon-gamma