title: Immunoglobulin VH gene expression in Ly-1+ and conventional B lymphocytes.
abstract: Lymphocyte populations in which Ly-1 B cells are differentially represented were studied for the expression of ten VH gene families, either by an RNA colony blot assay or by in situ hybridization of single cells, in BALB/c and C57BL/6 mice. The comparisons of cells from lymph nodes, Peyer's patches and adult spleen (poor in Ly-1 B cells) with cells from peritoneal cavity and neonatal spleen (rich in Ly-1 B cells) were confirmed by the analysis of adult peritoneal Ly-1- and Ly-1+ B cells sorted on the fluorescence-activated cell sorter. The results indicate that the peritoneal Ly-1+ B subset uses the whole spectrum of known VH gene families, and shows a preferential utilization of CP12 VH genes, most likely as a result of a selective process during life.
authors.affiliation: Département d'Immunologie, Institut Pasteur, Paris, France.
authors.forename: L
authors.initials: L
authors.lastname: Andrade
authors.forename: A A
authors.initials: AA
authors.lastname: Freitas
authors.forename: F
authors.initials: F
authors.lastname: Huetz
authors.forename: P
authors.initials: P
authors.lastname: Poncet
authors.forename: A
authors.initials: A
authors.lastname: Coutinho
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn / Q000276:immunology; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis; D000950:Antigens, Ly / Q000032:analysis; D001402:B-Lymphocytes / Q000145:classification / Q000502:physiology*; D005434:Flow Cytometry; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D008221:Lymphoid Tissue / Q000502:physiology; D051379:Mice; D008815:Mice, Inbred Strains; D009693:Nucleic Acid Hybridization; D013154:Spleen / Q000502:physiology
chemical_list: D000944:Antigens, Differentiation, B-Lymphocyte; D000950:Antigens, Ly; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region