title: Acute experimental glomerulonephritis induced by the glomerular deposition of circulating polymeric IgA-concanavalin A complexes.
abstract: The perfusion of polymeric or secretory IgA-Concanavalin A complexes into the aorta of rats led to a mannose-dependent binding of both IgA and lectin to the glomerular capillary wall, as shown by double immunolocalization experiments, by quantitative analysis of the amount of radiolabelled complexes bound per g of kidney, and by blocking experiments with the corresponding carbohydrate. Rats injected with amounts of those complexes as low as 500 micrograms developed, one hour later, a focal and segmental proliferative glomerulonephritis characterized by the deposition of injected complexes and of rat C3 and rat fibrin/fibrinogen in most glomeruli; focal thrombosis and small areas of necrosis in 10 to 15% of glomeruli, confined to the periphery of a single lobule of the tuft and segmental infiltration of these glomeruli by polymorphonuclear leucocytes and platelets. At the same time, many mesangial cells exhibited a hyperactive appearance, and red blood cells were noted in tubular lumens. In contrast, rats similarly injected with either monomeric IgA-ConA complexes, multimeric or secretory IgA-peanut agglutinin complexes or polymeric or monomeric IgA aggregates of comparable apparent molecular weight did not develop obvious glomerular lesions within one hour. The data indicate that performed polymeric IgA-ConA complexes can specifically bind to glomerular structures in vivo and trigger acute glomerular lesions locally, analogous to those observed in some glomerular diseases associated with a cryoglobulinaemia.
authors.affiliation: Department of Pediatrics, State University of Liège, Belgium.
authors.forename: J C
authors.initials: JC
authors.lastname: Davin
authors.forename: C
authors.initials: C
authors.lastname: Dechenne
authors.forename: J
authors.initials: J
authors.lastname: Lombet
authors.forename: B
authors.initials: B
authors.lastname: Rentier
authors.forename: J B
authors.initials: JB
authors.lastname: Foidart
authors.forename: P R
authors.initials: PR
authors.lastname: Mahieu
mesh_terms: D000208:Acute Disease; D000818:Animals; D003208:Concanavalin A / Q000097:blood / Q000378:metabolism*; D005260:Female; D005455:Fluorescent Antibody Technique; D005921:Glomerulonephritis / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D007070:Immunoglobulin A / Q000097:blood / Q000378:metabolism*; D007678:Kidney Glomerulus / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D011108:Polymers / Q000097:blood / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D007070:Immunoglobulin A; D011108:Polymers; D003208:Concanavalin A