Human sphingolipid activator protein-1 and sphingolipid activator protein-2 are encoded by the same gene.
Mixed oligonucleotide primers complementary to the translation product of the sphingolipid activator protein (SAP)-2 were used to generate a 144-base pair (bp) complementary DNA (cDNA). This cDNA probe was used to isolate a 2,649-nucleotide-long cDNA that was sequenced and found to contain coding sequences for two known activators of lysosomal enzymes, namely, the sphingolipid activator protein (SAP)-1 and SAP-2. The cDNA contains an open reading frame of 1,482 nucleotides and 1,167 nucleotides of 3'-nontranslated region, followed by a stretch of 24 residues of adenylic acid. At 20 nucleotides upstream from the poly(A) tail there is a consensus AATAAA polyadenylation signal that is preceded by another potential polyadenylation signal. The cDNA, designed SAP-1/SAP-2 cDNA, hybridizes with two human mRNA species of approximately 3 kb in length, which most probably arise from polyadenylation at different sites. There are higher amounts of steady-state RNA levels of the SAP-1/SAP-2 mRNA in skin fibroblasts in comparison to B cells. The steady-state SAP-1/SAP-2 mRNA levels in Gaucher B cells are higher than in their normal counterparts. There is one human SAP-1/SAP-2 gene that has been cloned and is localized on two approximately 5 kb BamHI fragments.
Reiner
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O
Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel.
Dagan
O
O
Horowitz
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M
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D015723:Gene Library; D005796:Genes; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010920:Placenta / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D015183:Restriction Mapping; D049231:Saposins; D012689:Sequence Homology, Nucleic Acid; D049128:Sphingolipid Activator Proteins; D013107:Sphingolipids / Q000378:metabolism
D006023:Glycoproteins; D015345:Oligonucleotide Probes; C483345:PSAP protein, human; D049231:Saposins; D049128:Sphingolipid Activator Proteins; D013107:Sphingolipids; D012313:RNA; D004247:DNA