title: Mutation mapping of the 2B5 ecdysone locus in Drosophila melanogaster reveals a long-distance controlling element.
abstract: Mutations at the Broad-Complex, a key gene triggering metamorphosis in Drosophila melanogaster, were mapped at the DNA level. The Broad-Complex includes two mutually complementing allelic classes, br and l(1)2Bc, both of which can associate to insertions at the same intron. Alterations spreading over 50 kilobases upstream from the transcription unit also disrupt br but not l(1)2Bc function.
authors.affiliation: Departamento de Biología Molecular, Centro de Biología Molecular, Universidad Autónoma de Madrid, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Sampedro
authors.forename: J
authors.initials: J
authors.lastname: Galceran
authors.forename: M
authors.initials: M
authors.lastname: Izquierdo
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping; D004252:DNA Mutational Analysis; D004331:Drosophila melanogaster / Q000235:genetics*; D004440:Ecdysone / Q000235:genetics*; D008675:Metamorphosis, Biological; D010641:Phenotype; D012045:Regulatory Sequences, Nucleic Acid
chemical_list: D004440:Ecdysone