title: Pathogenicity of Mycobacterium avium for human monocytes: absence of macrophage-activating factor activity of gamma interferon.
abstract: Mycobacterium avium is a frequent opportunistic pathogen in the acquired immunodeficiency syndrome (AIDS). We compared 12 strains of M. avium in an in vitro model of pathogenicity. Peripheral blood-derived monocytes from healthy individuals were infected with M. avium in vitro. Bacterial uptake and intracellular replication were assessed by microscopic count of acid-fast bacilli and CFU of bacteria, respectively, in lysed monocytes. The CFU assay showed that among five AIDS-associated strains, only one replicated in monocytes. Two of seven non-AIDS-associated strains replicated intracellularly. In addition, we examined the effect of gamma interferon (IFN-gamma) on M. avium infection. IFN-gamma treatment of monocytes decreased phagocytosis and had no effect on the intracellular replication of M. avium. Thus, most strains of M. avium do not multiply within monocytes from healthy individuals and IFN-gamma does not have macrophage-activating factor activity for M. avium infection of human monocytes.
authors.affiliation: Department of Medicine, Case Western Reserve University, Cleveland, Ohio.
authors.forename: H
authors.initials: H
authors.lastname: Toba
authors.forename: J T
authors.initials: JT
authors.lastname: Crawford
authors.forename: J J
authors.initials: JJ
authors.lastname: Ellner
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology; D015169:Colony Count, Microbial; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D007424:Intracellular Fluid / Q000187:drug effects / Q000382:microbiology; D008222:Lymphokines; D016215:Macrophage-Activating Factors; D009000:Monocytes / Q000187:drug effects / Q000382:microbiology*; D009162:Mycobacterium avium / Q000187:drug effects / Q000254:growth & development / Q000472:pathogenicity*
chemical_list: D008222:Lymphokines; D016215:Macrophage-Activating Factors; D007371:Interferon-gamma