Monoclonal antibodies against species-specific antigens in the chick central nervous system: putative application as transplantation markers in the chick-quail chimera.
With the recent progress in transplantation of neuronal tissues, cellular markers are needed to distinguish the grafted cells from the host. To generate monoclonal antibodies (MAb) recognizing species-specific antigens in the chick nervous system, we immunized mice with chick optic nerves and obtained 2 MAb which bind to chick but not to quail neural tissues. MAb-39B11 recognizes the cell surface antigen on the nerve fibers. MAb-37F5 recognizes the cytoplasmic components in several cell types, including ependymal cells and some large neurons. The utility of these MAb as markers for chick cells in the chick-quail chimeric brain and their advantages over conventional markers are discussed.
Takagi
S
S
Department of Anatomy, Kyoto Prefectural University of Medicine, Japan.
Tsuji
T
T
Kinutani
M
M
Fujisawa
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D015153:Blotting, Western; D002478:Cells, Cultured; D002645:Chickens / Q000276:immunology*; D002678:Chimera; D003370:Coturnix / Q000276:immunology; D005455:Fluorescent Antibody Technique; D007124:Immunoenzyme Techniques; D007381:Intermediate Filament Proteins / Q000276:immunology*; D008970:Molecular Weight; D009420:Nervous System / Q000033:anatomy & histology / Q000276:immunology*; D016900:Neurofilament Proteins; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D007381:Intermediate Filament Proteins; D016900:Neurofilament Proteins