Fixation and repair of radiation-induced potentially mutagenic damage sensitive to hypertonic treatment in human diploid fibroblasts.
The effect of hypertonic salt treatment on the repair of potentially lethal damage and potentially mutagenic damage in X-irradiated asynchronous and synchronous human diploid fibroblasts (IMR91) have been studied. Resistance to 6-thioguanine was used for the mutagenic end point. When cells in late-S-phase were treated with hypertonic salt solution immediately after X-irradiation, both cell killing and mutation induction were enhanced, as compared to X-irradiation alone. This suggests that X-irradiation of cells in late S phase induces both potentially lethal damage and potentially mutagenic damage and that both are sensitive to hypertonic salt solution. When cells were allowed 2 h for repair after exposure to X-rays, both types of damage were completely repaired. Almost the same results were obtained with asynchronous cells. These results are discussed in terms of the relationship between radiation damage leading to cell lethality and mutagenesis.
Kubota
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Albert Soiland Cancer Research Laboratory, University of Southern California School of Medicine, Los Angeles 90015.
Hill
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CK
Elkind
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D016590:Aphidicolin; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004260:DNA Repair / Q000187:drug effects*; D004261:DNA Replication / Q000187:drug effects / Q000528:radiation effects*; D004171:Diploidy; D004224:Diterpenes; D005347:Fibroblasts / Q000187:drug effects / Q000528:radiation effects*; D006801:Humans; D007399:Interphase / Q000187:drug effects / Q000528:radiation effects; D008297:Male; D009154:Mutation; D012462:Saline Solution, Hypertonic; D012965:Sodium Chloride
D004224:Diterpenes; D012462:Saline Solution, Hypertonic; D016590:Aphidicolin; D012965:Sodium Chloride