Salmonella typhimurium metC operator-constitutive mutations.
We used an Escherichia coli lac deletion strain lysogenized with a metC-lacZ fusion phage (lambda Clac) to select operator-constitutive mutations in the Salmonella typhimurium metC gene control region. The mutations were located in a region containing 2 tandemly repeated 8 bp palindromes previously proposed to be the MetJ repressor binding site. Lysogens carrying lambda Clac mutant phage exhibit high beta-galactosidase levels that are only partially repressible by methionine. The results suggest that the mutations disrupt the methionine control system mediated by the metJ gene product.
Park
Y M
YM
Department of Microbiology, University of Iowa, Iowa City 52242.
Stauffer
G V
GV
D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D008715:Methionine / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D009875:Operator Regions, Genetic; D010957:Plasmids; D012097:Repressor Proteins / Q000378:metabolism; D012486:Salmonella typhimurium / Q000235:genetics* / Q000302:isolation & purification; D001616:beta-Galactosidase / Q000235:genetics
D004269:DNA, Bacterial; D012097:Repressor Proteins; D008715:Methionine; D001616:beta-Galactosidase