Identification of a virulence factor for Brucella abortus infection in BALB/c mice.
Immunogenic or pathogenic factors of recombinant proteins (rBCSP20, rBCSP-31, and rBCSP45 of Brucella abortus strain 19) for mice were compared with factors of a proteinase K-treated lipopolysaccharide extracted from B abortus strain 2308. Mice were vaccinated with 4 products, using different inoculation schedules and were challenge exposed with a virulent culture of B abortus strain 2308. Blood samples were collected 2 weeks after vaccination and at necropsy and sera were obtained. Spleens were cultured for B abortus at necropsy (3 to 4 weeks after challenge exposure). Mice given proteinase K-treated lipopolysaccharide alone or in conjunction with rBCSP20 or rBCSP45 proteins were protected, but mice given rBCSP31 on the same day as challenge exposure were not. Vaccination with recombinant proteins alone neither provide protection nor significantly (P greater than 0.05) increase the pathogenic effect of the challenge-exposure culture. Seemingly, rBCSP31 might be a virulence factor of B abortus.
Pugh
G W
GW
US Department of Agriculture, National Animal Disease Center, Ames, IA 50010.
Tabatabai
L B
LB
Bricker
B J
BJ
Mayfield
J E
JE
Phillips
M
M
McDonald
T J
TJ
Zehr
E S
ES
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D001426:Bacterial Proteins / Q000276:immunology / Q000502:physiology*; D002003:Brucella abortus / Q000276:immunology / Q000302:isolation & purification / Q000472:pathogenicity*; D002006:Brucellosis / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D019286:Endopeptidase K; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D008070:Lipopolysaccharides / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000276:immunology; D012697:Serine Endopeptidases / Q000378:metabolism; D014774:Virulence
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D012697:Serine Endopeptidases; D019286:Endopeptidase K