Monoclonal antibodies specific for variable and constant domains of murine lambda chains.
Rat monoclonal antibodies directed against the BALB/c myeloma protein M315 (alpha,lambda 2) are described. 9A8 (IgG1) binds the V domain of lambda 2 and cross-reacts with lambda 1 and lambda 3 chains. 2B6 (IgG2a) is directed to the C domain of lambda 2 and cross-reacts with C lambda 3. The antibodies bind isolated chains as well as complete immunoglobulins. The monoclonals detect soluble immunoglobulin (radioimmunoassay), immunoglobulin immobilized on polystyrene (enzyme-linked immunosorbent assay), immunoglobulin bound to nitrocellulose (immunoblotting), and surface immunoglobulin intercalated in cell membranes (immunofluorescence). The antibodies are easily purified on protein G immunosorbents and may be biotinylated or conjugated with fluorescein isothiocyanate without loss of capacity to bind. In addition to the anti-lambda antibodies, a C alpha 2/C alpha 3-specific monoclonal antibody, 8D2 (IgG2a) is described.
Bogen
B
B
Institute for Immunology and Rheumatology, University of Oslo, Norway.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006825:Hybridomas / Q000032:analysis; D007127:Immunoglobulin Constant Regions / Q000032:analysis / Q000276:immunology*; D007135:Immunoglobulin Variable Region / Q000032:analysis / Q000276:immunology*; D007146:Immunoglobulin lambda-Chains / Q000032:analysis / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D011919:Rats, Inbred Strains
D000911:Antibodies, Monoclonal; D007127:Immunoglobulin Constant Regions; D007135:Immunoglobulin Variable Region; D007146:Immunoglobulin lambda-Chains