title: Experimental pancreatic preservation prior to islet isolation and transplantation.
abstract: Preservation of the Lewis rat pancreas prior to islet isolation was accomplished by initial intraductal distension with the University of Wisconsin (UW) hydroxyethyl starch-lactobionate solution to which collagenase had been added, followed by simple cold storage at 4 degrees C for 0, 3, 12, 24, and 48 hr (n = 16-21 at each interval). The pancreases were then processed by digestion and mechanical dispersion to produce free islets of Langerhans. The mean islet yields (+/- standard errors of the means) were controls = 819 +/- 58 (n = 21), 3 hr = 867 +/- 51 (n = 20), 12 hr = 770 +/- 71 (n = 16), 24 hr = 805 +/- 62 (n = 18), and 48 hr = 722 +/- 55 (n = 16). None of these means differed significantly. The islets from pairs of donor pancreases (mean dose of islets = 1586 +/- 72) were transplanted intraportally into single isogeneic recipients with streptozotocin-induced diabetes (plasma glucose greater than 400). The preservation interval directly influenced the outcome of these islet isografts in the following manner: (i) Rates of functional success (nonfasting glucose less than 200 mg/dl) were 100% after storage times of 0 hr (n = 10), 3 hr (n = 8), and 12 hr (n = 8); 86% with a storage time of 24 hr (n = 7); and 0% after 48 hr (n = 8). (ii) Return of euglycemia was increasingly delayed with increasing preservation intervals.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Surgery, University of Minnesota, Minneapolis 55455.
authors.forename: M J
authors.initials: MJ
authors.lastname: Field
authors.forename: D E
authors.initials: DE
authors.lastname: Sutherland
authors.forename: S R
authors.initials: SR
authors.lastname: Munn
mesh_terms: D000818:Animals; D002470:Cell Survival; D005947:Glucose / Q000378:metabolism*; D005951:Glucose Tolerance Test; D016381:Islets of Langerhans Transplantation; D008297:Male; D009926:Organ Preservation / Q000379:methods*; D010179:Pancreas; D051381:Rats; D011917:Rats, Inbred Lew; D013997:Time Factors
chemical_list: D005947:Glucose