Cloned Schistosoma mansoni proteinase (hemoglobinase) as a putative serodiagnostic reagent.
Expressed cDNA encoding a proteolytic enzyme from Schistosoma mansoni has been cloned recently. Circulating antibodies reacting with the recombinant protein have been detected in the blood of mice and humans infected with S. mansoni, S. japonicum, or S. haematobium. S. mansoni and S. haematobium infection can be distinguished by antibody titer.
Chappell
C L
CL
Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030.
Hackel
J
J
Davis
A H
AH
D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis*; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology; D003001:Cloning, Molecular; D003429:Cross Reactions; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000276:immunology*; D015801:Helminth Proteins; D006801:Humans; D015151:Immunoblotting; D051379:Mice; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D012550:Schistosoma mansoni / Q000201:enzymology* / Q000235:genetics / Q000276:immunology; D012555:Schistosomiasis mansoni / Q000175:diagnosis*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015801:Helminth Proteins; D011994:Recombinant Proteins; D003546:Cysteine Endopeptidases