PMN stimulation by factors from IL-1-treated human synovial cell cultures.
Following exposure of cultured human synovial cells to human recombinant interleukin 1 alpha (IL-1 alpha), we demonstrate the appearance of factors in the supernatant which stimulate human polymorphonuclear leukocyte (PMN) locomotion and elevate intracellular free calcium ([Ca++]i). The production of these factors can be abolished by actinomycin D or dexamethasone but not by cyclo-oxygenase or lipoxygenase inhibitors. In vivo, the supernatant induces a rapid accumulation of PMNs in rabbit skin following intradermal injection. These activities were not due to IL-1 itself, tumour necrosis factor (TNF alpha) or granulocyte-macrophage colony-stimulating factor (GM-CSF). Such factors may play an important role in inflammatory responses involving IL-1.
Watson
M L
ML
Department of Pharmacology, Royal College of Surgeons, London, UK.
Lewis
G P
GP
Westwick
J
J
D000818:Animals; D002478:Cells, Cultured; D016861:Cyclooxygenase Inhibitors; D003609:Dactinomycin / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000494:pharmacology*; D016859:Lipoxygenase Inhibitors; D009504:Neutrophils / Q000187:drug effects*; D011817:Rabbits; D011994:Recombinant Proteins / Q000494:pharmacology*; D013583:Synovial Membrane / Q000166:cytology* / Q000187:drug effects
D016861:Cyclooxygenase Inhibitors; D007375:Interleukin-1; D016859:Lipoxygenase Inhibitors; D011994:Recombinant Proteins; D003609:Dactinomycin; D003907:Dexamethasone