title: Sample preparation for the HPLC analysis of drugs in biological fluids.
abstract: Sample preparation for the analysis of drugs in biological fluids consists of a number of unit operations that are used for (i) release of the drug from a conjugate or biological matrix; (ii) removal of endogenous compounds that could interfere with the assay; and (iii) techniques for liquid handling. The trends in sample preparation that have occurred over the past 10 years in the authors' laboratory are discussed. In general, there has been a move from the traditional liquid-liquid extraction to methods using bonded-silica which permit rapid throughput and efficient extraction. Automation of sample preparation has seen further gains in productivity; however, the present generation of equipment lack the control and communication systems that are essential for the development of the automated integrated laboratory of the future.
authors.affiliation: Department of Drug Analysis, Smith Kline and French Research Ltd., Welwyn, Hertfordshire, UK.
authors.forename: R D
authors.initials: RD
authors.lastname: McDowall
authors.forename: E
authors.initials: E
authors.lastname: Doyle
authors.forename: G S
authors.initials: GS
authors.lastname: Murkitt
authors.forename: V S
authors.initials: VS
authors.lastname: Picot
mesh_terms: D001331:Automation; D001826:Body Fluids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D004364:Pharmaceutical Preparations / Q000032:analysis*; D013048:Specimen Handling
chemical_list: D004364:Pharmaceutical Preparations