CD8+ T lymphocyte control of HIV replication in cultured CD4+ cells varies among infected individuals.
Production of the human immunodeficiency virus (HIV) by cultured peripheral blood mononuclear cells (PMC) from many seropositive individuals is inhibited by the presence of CD8+ T lymphocytes. In a study of 10 subjects, high levels of virus replication could be detected in cultures of purified CD4+ cells, but not in unseparated PMC. Addition of highly purified, autologous CD8+ cells to the enriched CD4+ cells resulted in a dose-dependent inhibition of HIV growth and revealed that for some individuals, even low numbers of CD8+ cells can prevent replication of the virus. The data also indicated that culturing enriched CD4+ cells could greatly enhance detection of infectious virus in blood specimens and demonstrated that the CD4+ molecule is expressed on infected T cells isolated directly from the peripheral blood.
Walker
C M
CM
Department of Medicine, University of California, School of Medicine, San Francisco 94143.
Moody
D J
DJ
Stites
D P
DP
Levy
J A
JA
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis*; D016827:CD8 Antigens; D002478:Cells, Cultured; D006678:HIV / Q000254:growth & development*; D006801:Humans; D008297:Male; D013601:T-Lymphocytes / Q000276:immunology* / Q000382:microbiology; D014779:Virus Replication
D000945:Antigens, Differentiation, T-Lymphocyte; D016827:CD8 Antigens