Cloning and expression of Pseudomonas aeruginosa flagellin in Escherichia coli.
The flagellin gene was isolated from a Pseudomonas aeruginosa PAO1 genomic bank by conjugation into a PA103 Fla- strain. Flagellin DNA was transferred from motile recipient PA103 Fla+ cells by transformation into Escherichia coli. We show that transformed E. coli expresses flagellin protein. Export of flagellin to the E. coli cell surface was suggested by positive colony blots of unlysed cells and by isolation of flagellin protein from E. coli supernatants.
Kelly-Wintenberg
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Department of Microbiology, University of Tennessee, Knoxville 37996-0845.
Montie
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D001426:Bacterial Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000235:genetics*; D005408:Flagellin / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D005798:Genes, Bacterial; D008854:Microscopy, Electron; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000648:ultrastructure; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification
D001426:Bacterial Proteins; D011994:Recombinant Proteins; D005408:Flagellin