In vitro infection of bovine alloreactive cytotoxic T cell lines with sporozoites of Theileria annulata and T parva.
Bovine alloreactive cytotoxic lymphocyte (CTL) lines of known target specificity were infected in vitro with sporozoites of Theileria annulata and T parva and cultured in limiting dilution. The phenotypes of the CTL lines both pre- and post infection were assessed using a panel of monoclonal antibodies specific for defined bovine lymphocyte subpopulations. The effector function of the resultant infected cell lines was determined using a Cr51 release assay and compared to the uninfected control CTL line. The results indicated that T parva sporozoites consistently infected and transformed the CTL lines very efficiently even at the lowest cell doses. In contrast the T annulata sporozoites were largely unable to infect and transform the alloreactive CTL except at the very highest cell and sporozoite doses. A factor which appeared to influence susceptibility to T annulata infection was an increased level of class II expression on the CTL line. None of the cell lines showed cytotoxic effector function after infection with either T annulata or T parva sporozoites.
Innes
E A
EA
Institute of Animal Physiology and Genetics Research, Edinburgh Research Station.
Millar
P
P
Glass
E J
EJ
Brown
C G
CG
Spooner
R L
RL
D000818:Animals; D016782:Apicomplexa; D002417:Cattle / Q000097:blood*; D002460:Cell Line; D013602:T-Lymphocytes, Cytotoxic / Q000469:parasitology*; D013801:Theileriasis