title: Effective method for obtaining long nucleotide chains on partially complementary templates. Processed bovine gamma-interferon gene obtained from human gamma-interferon gene.
abstract: The method of obtaining the bovine gamma-interferon gene by means of simultaneous multidirected mutagenesis of the human gamma-interferon gene is presented. The first strand of the bovine gamma-interferon gene was obtained by ligation of synthetic oligonucleotides, using the cDNA of human gamma-interferon, cloned in the single-stranded phage M13mp19 as a template. The second strand was synthesized using a large fragment of E. coli DNA-polymerase I. The double-stranded gene was then treated by restriction nucleases and cloned in a pUC-18 derived vector. The primary structure was confirmed by sequencing.
authors.affiliation: M.M. Shemyakin Institute of Bioorganic Chemistry, Moscow, USSR.
authors.forename: V M
authors.initials: VM
authors.lastname: Rostapshov
authors.forename: I P
authors.initials: IP
authors.lastname: Chernov
authors.forename: T L
authors.initials: TL
authors.lastname: Azhikina
authors.forename: A M
authors.initials: AM
authors.lastname: Borodin
authors.forename: E D
authors.initials: ED
authors.lastname: Sverdlov
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000138:chemical synthesis*; D012689:Sequence Homology, Nucleic Acid; D013698:Templates, Genetic
chemical_list: D009841:Oligonucleotides; D007371:Interferon-gamma