ELISA test for the serodiagnosis of Akabane virus infection in cattle.
A simple ELISA test has been developed for the detection of IgG and IgM antibodies to Akabane virus in bovine serum. The test is specific and its sensitivity higher than the serum neutralisation assay. Detection of IgM antibodies can serve as a rapid method of diagnosing primary infection with Akabane virus. The superiority of ELISA resides mainly in the rapidity of performance and that it can be performed with inactivated reagents at high dilutions of serum samples. Thus it might enable the surveillance of spread of infection in zones prone to be affected by insect-borne viral diseases.
Ungar-Waron
H
H
Department of Immunology, Kimron Veterinary Institute, Beit Dagan, Israel.
Gluckman
A
A
Trainin
Z
Z
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002043:Bunyaviridae / Q000276:immunology*; D002044:Bunyaviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D009500:Neutralization Tests; D011237:Predictive Value of Tests; D012840:Simbu virus / Q000276:immunology*; D013997:Time Factors
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M