Cytotoxicity of arachidonic acid and of its lipoxygenase metabolite 15-hydroperoxyeicosatetraenoic acid on human breast cancer MCF-7 cells in culture.
Monolayer cultures of MCF-7 human breast cancer cell line were treated with arachidonic acid (AA) and 15-L-(s)-hydroperoxyeicosatetraenoic acid (15-L-(s)-HPETE) in a concentration range of 10(-5)-10(-11) M and their relative cytotoxic potential was determined. Both compounds had a time and dose-dependent cytotoxic effect on MCF-7 cells. 15-L-(S)-HPETE, the 15-lipoxygenase product of AA was the most effective.
Najid
A
A
Laboratoire de Biochimie, Faculty de Pharmacie, Limoges, France.
Beneytout
J L
JL
Tixier
M
M
D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000188:drug therapy*; D002455:Cell Division / Q000187:drug effects; D006801:Humans; D015289:Leukotrienes / Q000494:pharmacology*; D008054:Lipid Peroxides / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D001095:Arachidonic Acids; D015289:Leukotrienes; D008054:Lipid Peroxides; D016718:Arachidonic Acid; C025086:15-hydroperoxy-5,8,11,13-eicosatetraenoic acid