Effect of DMBA on oral cancer development in hamsters with latent HSV-1 infections in trigeminal ganglia.
This study examined the effect of 7, 12-dimethyl-benz(a)anthracene (DMBA), polycyclic aromatic hydrocarbon carcinogen, on pathologic changes in buccal pouch mucosa of hamsters with latent herpes simplex virus type 1 (HSV-1) infections in their trigeminal ganglia. Of the pouches receiving DMBA treatment, the average number of tumors per pouch was significantly higher (p less than 0.01) in pouches of animals with HSV-1 infections as compared to those that received DMBA only. Of the pouches receiving DMBA, the average cumulative tumor diameter (the sum of the tumor diameters in each group divided by the number of pouches in that group) was notably greater in animals with latent HSV-1 than in animals that had not been infected; however, this difference was not significant. The DMBA-treated hamsters that had latent infections also displayed a higher severity and prevalence of histopathologic changes in their pouch mucosa. This study indicates that latent HSV-1 and DMBA show synergism in the development of tumors and precancerous histopathologic changes in hamster buccal pouch mucosa. An unexpected finding was that among the HSV-1 infected animals, 95% of the ganglia from animals treated with DMBA showed latent HSV-1 virus on explanation culture, whereas only 10% of the ganglia from infected animals that received mineral oil, rather than DMBA, contained latent virus.
Cornella
F A
FA
Section of Oral Biology, School of Dentistry, University of California, Los Angeles.
Saper
C D
CD
Christensen
R E
RE
Park
N H
NH
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D003043:Cocarcinogenesis; D006224:Cricetinae; D006561:Herpes Simplex / Q000503:physiopathology*; D009061:Mouth Mucosa / Q000473:pathology; D009062:Mouth Neoplasms / Q000139:chemically induced / Q000209:etiology* / Q000503:physiopathology; D014276:Trigeminal Nerve / Q000473:pathology*
D015127:9,10-Dimethyl-1,2-benzanthracene