title: Immunodiagnosis of human and porcine cysticercosis. Detection of antibodies and parasite products.
abstract: In this paper we report the immunodiagnosis of cysticercosis by the detection of antibodies in the cerebrospinal fluid (CSF) of patients, by standard enzyme immunoassay (EIA) which gave 90% sensitivity. We are also showing the results obtained with a double homologous polyclonal or monoclonal antibody based EIA that detects parasite products: we analysed CSF of patients and obtained sensitivities ranging from 14% to 75% for single antibody systems and 90% when analysing the samples with two or three systems simultaneously. The sera of infected pigs were also evaluated; with one monoclonal antibody we could detect 79% of positive samples. The good results obtained encourage the standardization and improvement of the technique for diagnostic as well as for epidemiologic purposes.
authors.affiliation: Departamento de Immunologia, UNAM, México.
authors.forename: M D
authors.initials: MD
authors.lastname: Correa
authors.forename: A
authors.initials: A
authors.lastname: Plancarte
authors.forename: M A
authors.initials: MA
authors.lastname: Sandoval
authors.forename: E
authors.initials: E
authors.lastname: Rodriguez-del-Rosal
authors.forename: A
authors.initials: A
authors.lastname: Meza-Lucas
authors.forename: A
authors.initials: A
authors.lastname: Flisser
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000134:cerebrospinal fluid; D000947:Antigens, Helminth / Q000302:isolation & purification; D003551:Cysticercosis / Q000175:diagnosis* / Q000469:parasitology / Q000662:veterinary; D006801:Humans; D007159:Immunologic Tests; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000469:parasitology; D013621:Taenia / Q000276:immunology
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth