Comparison of different flow injection approaches to the automatic determination of enzymatic activity.
Three configurations based on the principles behind flow injection analysis (FIA) are proposed for the automatic determination of enzymatic activity. The proposed approaches are normal, stopped-flow and open-closed FIA. The comparative study of the methods developed from these approaches allows the establishment of the scope of the application of each, with inherent advantages and disadvantages.
Fernandez-Romero
J M
JM
Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, Spain.
Luque de Castro
M D
MD
Valcarcel
M
M
D004798:Enzymes / Q000032:analysis / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007770:L-Lactate Dehydrogenase / Q000032:analysis / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D009243:NAD / Q000378:metabolism; D011773:Pyruvates / Q000378:metabolism; D019289:Pyruvic Acid; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
D004798:Enzymes; D007202:Indicators and Reagents; D007773:Lactates; D011773:Pyruvates; D009243:NAD; D019344:Lactic Acid; D019289:Pyruvic Acid; D007770:L-Lactate Dehydrogenase