title: The formation of biologically active beta-galactosidase inclusion bodies in Escherichia coli.
abstract: Culture conditions affecting the formation of beta-galactosidase inclusion bodies in E. coli were examined. High temperature, early induction, high salt concentration and low aeration were all found to favour an increase of insoluble beta-galactosidase and the formation of visible inclusion bodies. The ratio of soluble to insoluble beta-galactosidase decreased during the course of cell growth. When assayed for beta-galactosidase activity, the inclusion bodies were enzymatically active with a specific activity of one third that of soluble beta-galactosidase. The activity remained associated with the inclusion bodies on washing with detergent and high ionic strength buffers. These results suggest that inclusion bodies can contain correctly folded protein.
authors.affiliation: Biotechnology Australia Pty Ltd.
authors.forename: D M
authors.initials: DM
authors.lastname: Worrall
authors.forename: N H
authors.initials: NH
authors.lastname: Goss
mesh_terms: D001431:Bacteriological Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology* / Q000648:ultrastructure; D001616:beta-Galactosidase / Q000096:biosynthesis*
chemical_list: D001616:beta-Galactosidase