A penicillinase test incorporated in the rapid carbohydrate fermentation method for rapid detection of beta-lactamase producing Neisseria gonorrhoeae.
A modification of the acidometric (phenol red) test for penicillinase producing N. gonorrhoeae was incorporated into the rapid fermentation method for rapid screening and identification of PPNG strains. Two hundred and twenty-four non-penicillinase-producing N. gonorrhoeae, 55 penicillinase-producing N. gonorrhoeae, 87 N. meningitidis and 89 N. lactamica were included in this study. Results of the modified test were comparable with the iodometric and penicillin disk diffusion susceptibility and were obtainable within 1 to 5 minutes.
Lesmana
M
M
U.S. Naval Medical Research Unit No. 2, Medical Faculty, Trisakti, University, Jakarta.
Lane
E M
EM
Tjaniadi
P
P
Rockhill
R C
RC
Leksana
B
B
Widjaja
U
U
D005285:Fermentation; D008828:Microbiological Techniques; D009344:Neisseria gonorrhoeae / Q000201:enzymology*; D010405:Penicillinase / Q000378:metabolism*
D010405:Penicillinase