title: Comparison of four screening techniques for the diagnosis of equine neonatal hypogammaglobulinemia.
abstract: Using radial immunodiffusion as a standard, 4 screening techniques for detection of failure of passive transfer in equine neonates were compared for sensitivity, specificity, positive and negative predictive values, efficiency, and cost. The techniques compared were latex agglutination test, membrane filter ELISA, dipstick ELISA, and glutaraldehyde coagulation (GC) test. Test results of 50 serum samples from foals 24 to 60 hours old revealed consistently highest accuracy in the GC test at IgG concentrations of 400 and 800 mg/dl, and lowest cost per test, using the GC test. Two hundred fifty-three serum samples from foals 24 to 60 hours old were evaluated for comparison of results of GC and radial immunodiffusion tests. Overall efficiency was 92 and 91% at serum IgG concentrations of 400 and 800 mg/dl, respectively. Under most field circumstances, the GC test would be the preferred screening test for detection of failure of passive transfer in equine neonates.
authors.affiliation: Department of Food Animal and Equine Medicine, School of Veterinary Medicine, North Carolina State University, Raleigh 27606.
authors.forename: D L
authors.initials: DL
authors.lastname: Clabough
authors.forename: H S
authors.initials: HS
authors.lastname: Conboy
authors.forename: M C
authors.initials: MC
authors.lastname: Roberts
mesh_terms: D000361:Agammaglobulinemia / Q000175:diagnosis / Q000662:veterinary*; D000818:Animals; D000831:Animals, Newborn; D004797:Enzyme-Linked Immunosorbent Assay; D006734:Horse Diseases / Q000175:diagnosis*; D006736:Horses; D005779:Immunodiffusion; D007074:Immunoglobulin G / Q000032:analysis; D007841:Latex Fixation Tests; D011237:Predictive Value of Tests
chemical_list: D007074:Immunoglobulin G