Differential L chain expression in the antibody responses to phosphorylcholine of adult bone marrow or peritoneum-derived B lymphocytes.
Lethal irradiation of adult BALB/c mice followed by reconstitution with autologous bone marrow results in loss of T15 Id and IdX expression in the responses to phosphorylcholine (PC) and alpha(1-3)-dextran, respectively. T15 Id, but not IdX expression can be reconstituted with low numbers of syngeneic, T cell-depleted peritoneal resident cells. All three groups of mice produce comparable titers of specific anti-PC and anti-dextran antibodies. The inability of adult bone marrow-reconstituted BALB/c mice to produce T15 Id+ antibodies is not due to differential VH-gene expression in bone marrow or peritoneum-derived B cells. Thus, the levels of T15 VH in total serum Ig and in anti-PC antibodies are similar in all groups of mice. Furthermore, IEF patterns of T15 VH-associated L chains directly demonstrate differential Vk repertoire expression in bone marrow and peritoneum-derived B cells.
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Département d'Immunologie, Institut Pasteur, Paris, France.
Martinez
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C
Coutinho
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D000375:Aging; D000818:Animals; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000637:transplantation; D001853:Bone Marrow / Q000276:immunology*; D016026:Bone Marrow Transplantation; D002794:Choline / Q000031:analogs & derivatives*; D007147:Immunoglobulin Light Chains / Q000302:isolation & purification*; D051379:Mice; D008807:Mice, Inbred BALB C; D009194:Myeloma Proteins / Q000378:metabolism; D010529:Peritoneal Cavity; D010767:Phosphorylcholine / Q000276:immunology*
D007147:Immunoglobulin Light Chains; D009194:Myeloma Proteins; C043925:myeloma protein TEPC15; D010767:Phosphorylcholine; D002794:Choline