Modulation of ovarian carcinoma tumor marker CA-125 by gamma-interferon.
Interferons are known to modulate several cellular functions by the induction of different proteins. In our study a gamma-interferon-mediated presentation of one of the most important ovarian tumor markers (CA-125) on the cell surface was demonstrated using two ovarian carcinoma cell lines in vitro (HTB-77 and SKOV-3). This induction was found to be dependent on an intact protein biosynthesis. The gamma-interferon effect reached a maximum on the third day of treatment. Under such conditions the CA-125 concentration was increased intracellularly, on the cell surface, and in the supernatant culture medium. The surface antigen was shed rapidly with a half-life of 1 day. The addition of dexamethasone to gamma-interferon treated HTB-77 cells improved CA-125 expression synergistically. HLA-DR and CA-125 expression was found to be regulated by interferons in different ways. The demonstration of CA-125 expression provides an important insight into the potential regulatory mechanism governing this tumor marker. Since interferons are naturally occurring substances as well as therapeutically administered agents, it seems necessary to pay attention to possible tumor marker modulation.
Marth
C
C
Department of Obstetrics and Gynecology, Innsbruck University Clinic, Austria.
Fuith
L C
LC
Böck
G
G
Daxenbichler
G
G
Dapunt
O
O
D015295:Antigens, Tumor-Associated, Carbohydrate / Q000276:immunology*; D003513:Cycloheximide / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D005260:Female; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology*; D010051:Ovarian Neoplasms / Q000276:immunology*; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured
D015295:Antigens, Tumor-Associated, Carbohydrate; D006684:HLA-DR Antigens; D007370:Interferon Type I; D011994:Recombinant Proteins; D003907:Dexamethasone; D007371:Interferon-gamma; D003513:Cycloheximide