Fractionation of trypanosome antigens for species-specific sero-diagnosis.
Crude somatic antigens from isolated and ultrasonically treated trypanosomes were fractionated by column chromatography. A protein-free antigenic fraction was isolated which reacted monospecifically when tested against hyperimmune sera from rabbits. The method has a potential application in the improvement of serodiagnosis of trypanosomiasis.
Ijagbone
I F
IF
Department of Veterinary Public Health and Preventive Medicine, University of Ibadan, Nigeria.
Staak
C
C
Reinhard
R
R
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis*; D005591:Chemical Fractionation; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D003168:Complement Fixation Tests; D004797:Enzyme-Linked Immunosorbent Assay; D005779:Immunodiffusion; D008297:Male; D051381:Rats; D013045:Species Specificity; D014346:Trypanosoma brucei brucei / Q000276:immunology*; D014348:Trypanosoma congolense / Q000276:immunology*; D014353:Trypanosomiasis, African / Q000175:diagnosis*
D000953:Antigens, Protozoan