title: Stage-specific inhibin secretion by rat seminiferous tubules.
abstract: The endogenous inhibin content was measured by radioimmunoassay in segments of seminiferous tubules at specific stages of the spermatogenic cycle isolated by transillumination-assisted dissection as described by Parvinen (1982). In addition, the immunoactive inhibin production in response to FSH stimulation was investigated. The inhibin content was greatest in tubules at stages II-VI of the cycle and was lowest at stages VII-VIII. Over 48 h of culture, the secretion of inhibin into the medium was greatest at stages XIII-VI and lowest at stages VII-VIII. The inhibin response to FSH stimulation was greatest at stages IX-XIII and least at stages VII-VIII. The results demonstrate that the secretion of inhibin by adult tubules in vitro varies according to the stage of the seminiferous cycle.
authors.affiliation: Department of Anatomy, Monash University, Vic, Australia.
authors.forename: G F
authors.initials: GF
authors.lastname: Gonzales
authors.forename: G P
authors.initials: GP
authors.lastname: Risbridger
authors.forename: Y H
authors.initials: YH
authors.lastname: Hodgson
authors.forename: P
authors.initials: P
authors.lastname: Pöllänen
authors.forename: D M
authors.initials: DM
authors.lastname: de Kretser
mesh_terms: D000818:Animals; D046508:Culture Techniques; D004305:Dose-Response Relationship, Drug; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D007265:Inhibins / Q000032:analysis / Q000378:metabolism*; D008297:Male; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D012671:Seminiferous Tubules / Q000187:drug effects / Q000378:metabolism*; D013091:Spermatogenesis / Q000502:physiology*; D013737:Testis / Q000378:metabolism*
chemical_list: D007265:Inhibins; D005640:Follicle Stimulating Hormone