Heterogeneous expression of S-100 protein subunits alpha and beta in cystadenolymphomas of salivary glands.
Immunohistochemical expression of S-100 protein and its subunits alpha and beta in a total of 27 cases of cystadenolymphomas of the parotid gland is described by means of polyclonal anti-S-100 protein antiserum and monoclonal antibodies against S-100 alpha and beta. Normal salivary glands showed strongly positive staining for S-100 alpha in acinic cells and a negative or slightly positive reaction in ductal cells; however, they were usually negative for S-100 beta staining. Neoplastic epithelial cells of cystadenolymphomas showed slightly positive staining for S-100 alpha in their cytoplasm, and there was a strongly positive reaction in a limited number of tumour cells. The S-100 beta subunit was expressed as a fine granular deposition in the apical cytoplasm of tumour cells. S-100 protein staining with the polyclonal reagent was usually negative or slightly positive in the tumour epithelia, but a small number of strongly positive cells were scattered throughout the tumour epithelia, suggesting the presence of Langerhan's cells. Interdigitating cells in lymphoid tissue reacted positively to monoclonal S-100 alpha as well as to the polyclonal antiserum.
Hosaka
M
M
Department of Oral Surgery, Asahi University School of Dentistry, Gifu, Japan.
Orito
T
T
Horike
H
H
Okada
Y
Y
Mori
M
M
D000235:Adenolymphoma / Q000032:analysis*; D000911:Antibodies, Monoclonal; D004848:Epithelium / Q000032:analysis; D006651:Histocytochemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D008221:Lymphoid Tissue / Q000032:analysis; D010307:Parotid Neoplasms / Q000032:analysis*; D009418:S100 Proteins / Q000032:analysis*; D012469:Salivary Glands / Q000032:analysis
D000911:Antibodies, Monoclonal; D009418:S100 Proteins