TSH, thyroid hormones and nuclear-binding of T3 in mononuclear blood cells from obese and non-obese women.
The specific nuclear-binding of T3 (NBT3) in mononuclear blood cells, and the concentrations of TSH, thyroid hormones, and binding proteins were measured after overnight fasting in 12 obese and in 14 non-obese women, none of the subjects were taking any medicine. The concentrations of TSH and free plus bound-T3 (TT3) were significantly higher in the obese (p less than 0.05), concentrations of T4 and binding proteins did not differ. The NBT3 was significantly lower in the obese women; the maximal binding capacity (MBC) was 34.5 +/- 11.6 fmol/mg DNA in the obese subjects and 50.0 +/- 11.6 fmol/mg DNA in the non-obese subjects (p less than 0.02). The binding affinities did not differ. We have previously shown that increasing T3 concentrations within the physiological range down-regulates NBT3. Therefore, the reduced NBT3 in the obese women was probably secondary to the increased TT3 concentration and was not caused by a primary tissue resistance. The higher TSH and TT3 in the obese women could be caused by a greater caloric intake.
Matzen
L E
LE
Department of Clinical Chemistry, Odense University Hospital, Denmark.
Kvetny
J
J
Pedersen
K K
KK
D000328:Adult; D002467:Cell Nucleus / Q000378:metabolism; D005260:Female; D006801:Humans; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D009765:Obesity / Q000097:blood*; D011228:Prealbumin / Q000378:metabolism; D011988:Receptors, Thyroid Hormone / Q000378:metabolism*; D012709:Serum Albumin / Q000378:metabolism; D013972:Thyrotropin / Q000097:blood*; D013975:Thyroxine-Binding Proteins / Q000378:metabolism
D011228:Prealbumin; D011988:Receptors, Thyroid Hormone; D012709:Serum Albumin; D013975:Thyroxine-Binding Proteins; D013972:Thyrotropin