title: Synthesis of buffers for generating immobilized pH gradients. I: Acidic acrylamido buffers.
abstract: Five acidic acrylamido buffers for isoelectric focusing in immobilized pH gradients, are described: a strong titrant (pK 1.0) 2-acrylamido-2-methyl propane sulphonic acid and four weaker acids, namely 2-acrylamido glycolic acid (pK 3.1), N-acryloil glycine (pK 3.6), 3-acrylamido propanoic acid (pK 4.4) and 4-acrylamido butyric acid (pK 4.6). Their synthesis and general properties are described.
authors.affiliation: Department of Biomedical Sciences and Technologies, University of Milano, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Chiari
authors.forename: E
authors.initials: E
authors.lastname: Casale
authors.forename: E
authors.initials: E
authors.lastname: Santaniello
authors.forename: P G
authors.initials: PG
authors.lastname: Righetti
mesh_terms: D000178:Acrylamides / Q000138:chemical synthesis* / Q000737:chemistry; D002021:Buffers; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing
chemical_list: D000178:Acrylamides; D002021:Buffers; C059413:Immobiline