title: Quantification of apolipoprotein A-1 in cow serum by single radial immunodiffusion.
abstract: A single radial immunodiffusion assay was developed to determine the concentration of the major apolipoprotein A-I in bovine serum. Assay was performed using agarose gel plates with monospecific rabbit antiserum. Standards made with bovine high density lipoproteins or serum 10 times diluted were applied in 3-microliters amounts to each well in the gel. The assay working range was 2 to 22 mg of apolipoprotein A-I. The intraassay and interassay CV were 3.7 and 4.9%, respectively. Using protein A-I. The intraassay and interassay CY were 3.7 and 4.9%, respectively. Using radial immunodiffusion assay, 23 serum samples from cows at various stages of lactation were analyzed. Apolipoprotein A-I was significantly increased in midlactation compared with concentrations in early lactation or dry period.
authors.affiliation: Laboratoire des Maladies Métaboliques, INRA, Theix, Ceyrat.
authors.forename: A
authors.initials: A
authors.lastname: Mazur
authors.forename: E
authors.initials: E
authors.lastname: Marcos
authors.forename: P
authors.initials: P
authors.lastname: Cardot
authors.forename: M
authors.initials: M
authors.lastname: Ayrault-Jarrier
authors.forename: Y
authors.initials: Y
authors.lastname: Rayssiguier
mesh_terms: D000818:Animals; D001054:Apolipoproteins A / Q000097:blood*; D002417:Cattle / Q000097:blood*; D005260:Female; D005779:Immunodiffusion / Q000662:veterinary; D007122:Immunoelectrophoresis
chemical_list: D001054:Apolipoproteins A