A comparison of intraperitoneal injection and oral gavage in the micronucleus test with mitomycin C in mice.
Intraperitoneal (i.p.) injection and oral (p.o.) gavage were evaluated in the mouse micronucleus test with mitomycin C (MMC). The tests were carried out in 2 laboratories with the MS/Ae and CD-1 mouse strains. On the basis of a small-scale acute toxicity study and a pilot experiment, the full-scale micronucleus test was performed with a 24-h sampling time at doses of 1, 2, 4, and 8 mg/kg for both treatment routes. In both strains, a clear positive dose-response relation was shown by both routes. Although the frequency of micronucleated polychromatic erythrocytes (MNPCEs) was higher with i.p. on a mg/kg basis, this tendency was reversed when dose was expressed as a percentage of the LD50.
Sato
S
S
Toxicology Research Laboratories, Japan Tobacco Inc., Kanagawa.
Inui
N
N
Ikeda
Y
Y
Hiraga
Y
Y
D000284:Administration, Oral; D000818:Animals; D007274:Injections, Intraperitoneal; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D016685:Mitomycin; D008937:Mitomycins / Q000008:administration & dosage* / Q000633:toxicity; D009153:Mutagens / Q000008:administration & dosage*; D010865:Pilot Projects
D008937:Mitomycins; D009153:Mutagens; D016685:Mitomycin