Quantitative analysis of chlortetracycline and related substances by high-performance liquid chromatography.
Isocratic high-performance liquid chromatography on Zorbax C8 7 microns allows quantitative determination of chlortetracycline, 4-epichlortetracycline, tetracycline, demethylchlortetracycline and isochlortetracycline using a mobile phase containing dimethylsulphoxide, 1 M perchloric acid and water (35:5:60). The minor impurities anhydrochlortetracycline and 4-epianhydrochlortetracycline, which are more strongly retained can be determined using a second isocratic system with a mobile phase containing more organic modifier. The method has been used for the comparison of official standards and for the analysis of a number of commercial samples.
Khan
N H
NH
Katholieke Universiteit Leuven, Instituut voor Farmaceutische Wetenschappen, Belgium.
Roets
E
E
Hoogmartens
J
J
Vanderhaeghe
H
H
D002751:Chlortetracycline / Q000031:analogs & derivatives* / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D007202:Indicators and Reagents; D012015:Reference Standards; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet
D007202:Indicators and Reagents; D012997:Solvents; D002751:Chlortetracycline