title: Evaluation of the Autoanalysis Colilert test for detection and enumeration of total coliforms.
abstract: The Autoanalysis Colilert (AC) test was compared with the membrane filter (MF), 10-tube multiple-tube fermentation (MTF) technique, and the presence-absence test as described in Standard Methods for the Examination of Water and Wastewater for the detection and enumeration of total coliforms in water. The methods were evaluated with 31 samples from seven different sources. Each sample was analyzed by each of the techniques, using replicate 100-ml sample volumes. A total of 582 confirmed tubes were positive by the MTF test, and 533 tubes were positive by the AC test. Statistical analysis of the most-probable-number comparability data showed a statistically significant difference in the number of positive tubes, with the MTF test resulting in more positive tubes. There were no statistically significant differences in precision between the two methods. All the methods were comparable in detection of total coliforms. Levels of heterotrophic bacteria generally encountered in drinking water did not interfere with detection or enumeration of coliforms by the AC test.
authors.affiliation: Environmental Monitoring Systems Laboratory, U.S. Environmental Protection Agency, Cincinnati, Ohio.
authors.forename: T C
authors.initials: TC
authors.lastname: Covert
authors.forename: L C
authors.initials: LC
authors.lastname: Shadix
authors.forename: E W
authors.initials: EW
authors.lastname: Rice
authors.forename: J R
authors.initials: JR
authors.lastname: Haines
authors.forename: R W
authors.initials: RW
authors.lastname: Freyberg
mesh_terms: D015169:Colony Count, Microbial / Q000379:methods*; D004755:Enterobacteriaceae / Q000201:enzymology / Q000254:growth & development* / Q000302:isolation & purification; D005456:Fluorescent Dyes / Q000378:metabolism; D006801:Humans; D006923:Hymecromone / Q000031:analogs & derivatives / Q000378:metabolism; D009598:Nitrophenylgalactosides / Q000378:metabolism; D014871:Water Microbiology; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D009598:Nitrophenylgalactosides; D006923:Hymecromone; C034888:4-methylumbelliferyl glucuronide; D001616:beta-Galactosidase