Construction and screening of Plasmodium falciparum cDNA library.
The HAINAN isolate of Plasmodium falciparum FCC1/HN was cultured in vitro in large quantities. The total parasite mRNA was purified and reverse transcribed into cDNA. The cDNA fragments were inserted into lambda gt11 to construct a P. falciparum FCC1/HN erythrocytic stage cDNA library. Inhibitory monoclonal antibodies (McAbs) M26-32, F6-C2, and F6-D3 were used to screen the cDNA library expressed in E. coli. A total of 27 positive clones were found to react with M26-32 alone and 34 clones with both M26-32 and F6-C2. These expressed proteins may be candidates for use in malaria vaccine.
Cheng
Q
Q
Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing.
Shen
Y F
YF
Qiang
B Q
BQ
Yu
S H
SH
Wu
N H
NH
Chen
J F
JF
Fan
R G
RG
Cheng
X K
XK
Li
W L
WL
Mao
Y H
YH
D000818:Animals; D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan / Q000032:analysis / Q000235:genetics; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D016054:DNA, Protozoan / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics; D015723:Gene Library; D010963:Plasmodium falciparum / Q000235:genetics* / Q000276:immunology; D012333:RNA, Messenger / Q000302:isolation & purification; D011995:Recombination, Genetic
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D012333:RNA, Messenger; D004247:DNA