title: Construction and screening of Plasmodium falciparum cDNA library.
abstract: The HAINAN isolate of Plasmodium falciparum FCC1/HN was cultured in vitro in large quantities. The total parasite mRNA was purified and reverse transcribed into cDNA. The cDNA fragments were inserted into lambda gt11 to construct a P. falciparum FCC1/HN erythrocytic stage cDNA library. Inhibitory monoclonal antibodies (McAbs) M26-32, F6-C2, and F6-D3 were used to screen the cDNA library expressed in E. coli. A total of 27 positive clones were found to react with M26-32 alone and 34 clones with both M26-32 and F6-C2. These expressed proteins may be candidates for use in malaria vaccine.
authors.affiliation: Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing.
authors.forename: Q
authors.initials: Q
authors.lastname: Cheng
authors.forename: Y F
authors.initials: YF
authors.lastname: Shen
authors.forename: B Q
authors.initials: BQ
authors.lastname: Qiang
authors.forename: S H
authors.initials: SH
authors.lastname: Yu
authors.forename: N H
authors.initials: NH
authors.lastname: Wu
authors.forename: J F
authors.initials: JF
authors.lastname: Chen
authors.forename: R G
authors.initials: RG
authors.lastname: Fan
authors.forename: X K
authors.initials: XK
authors.lastname: Cheng
authors.forename: W L
authors.initials: WL
authors.lastname: Li
authors.forename: Y H
authors.initials: YH
authors.lastname: Mao
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan / Q000032:analysis / Q000235:genetics; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D016054:DNA, Protozoan / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics; D015723:Gene Library; D010963:Plasmodium falciparum / Q000235:genetics* / Q000276:immunology; D012333:RNA, Messenger / Q000302:isolation & purification; D011995:Recombination, Genetic
chemical_list: D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D012333:RNA, Messenger; D004247:DNA