Molecular characterization of a gene encoding a photolyase from Streptomyces griseus.
By using a synthetic DNA probe derived from an amino acid sequence in the most conserved region of three known photolyases (Escherichia coli, Anacystis nidulans and Saccharomyces cerevisiae), we isolated a DNA fragment containing two long open reading frames (ORFs) from a genomic DNA library of Streptomyces griseus. One ORF encodes a polypeptide of 455 amino acids (Mr 50594), which exhibits substantial similarities with the other three photolyases. Photoreactivation-repair deficient E. coli cells could be converted into photoreactivatable ones by introduction of plasmids harboring this ORF, indicating that this is the photolyase gene of S. griseus. The deduced aa sequence of Streptomyces photolyase was most similar to that of E. coli. The putative DNA binding site as well as cofactor binding regions were proposed.
Kobayashi
T
T
Research Institute for Tuberculosis and Cancer, Tohoku University, Sendai, Japan.
Takao
M
M
Oikawa
A
A
Yasui
A
A
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000235:genetics* / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D005798:Genes, Bacterial; D005822:Genetic Vectors; D008190:Lyases / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012689:Sequence Homology, Nucleic Acid; D013305:Streptomyces griseus / Q000201:enzymology* / Q000235:genetics
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D008190:Lyases; D004255:Deoxyribodipyrimidine Photo-Lyase