title: Utilization of acetate in Escherichia coli: structural organization and differential expression of the ace operon.
abstract: Growth of Escherichia coli on acetate as the sole source of carbon and energy requires operation of the glyoxylate bypass in connection with the expression of the polycistronic ace operon. The structural organization of this operon is presented, including the 3 structural genes coding respectively for malate synthase (aceB), isocitrate lyase (aceA) and isocitrate dehydrogenase kinase/phosphatase (aceK), and the surrounding genes iclR and metA. In addition, the differential expression of genes aceB, aceA, and aceK has been tested both in vivo in a minicell system and in vitro in a plasmid-directed transcription-translation coupled system. Moreover, the codon usage and adaptation to transfer RNA frequencies during translation of the corresponding messenger RNAs have been measured.
authors.affiliation: Laboratoire de Biologie Moléculaire, Université de Lyon, France.
authors.forename: J C
authors.initials: JC
authors.lastname: Cortay
authors.forename: F
authors.initials: F
authors.lastname: Bleicher
authors.forename: B
authors.initials: B
authors.lastname: Duclos
authors.forename: Y
authors.initials: Y
authors.lastname: Cenatiempo
authors.forename: C
authors.initials: C
authors.lastname: Gautier
authors.forename: J L
authors.initials: JL
authors.lastname: Prato
authors.forename: A J
authors.initials: AJ
authors.lastname: Cozzone
mesh_terms: D000085:Acetates / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D005796:Genes; D009876:Operon; D010766:Phosphorylation; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D000085:Acetates; D012333:RNA, Messenger