Selective accumulation of a new antineoplastic agent, K-18 (human immunoglobulin conjugated melphalan), in Ehrlich carcinoma transplanted in mice.
The absorption of K-18 (human IgG conjugated melphalan) through the intestine and its selective accumulation in the tumor were studied. After oral administration of 14C-melphalan and 14C-K-18 to ICR mice with subcutaneously transplanted Ehrlich carcinoma, K-18 was detected at the tumor site by autoradiogram after 48 hrs, but melphalan was not. Immunofluorescence study showed that orally administered K-18 was absorbed from the intestine via both the portal and the lymphatic route. Moreover, immunoperoxidase staining revealed K-18 in cytoplasm of tumor cells, but not in bone marrow cells. These results demonstrate the absorption of K-18 from the intestine after oral administration and its selective affinity for tumor tissue.
Nio
Y
Y
First Department of Surgery, School of Medicine, Kyoto University, Japan.
Imai
S
S
Ohgaki
K
K
Tobe
T
T
D000818:Animals; D002250:Carbon Radioisotopes; D002286:Carcinoma, Ehrlich Tumor / Q000000981:diagnostic imaging / Q000378:metabolism* / Q000473:pathology; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G; D000922:Immunotoxins; D008558:Melphalan / Q000493:pharmacokinetics*; D051379:Mice; D008813:Mice, Inbred ICR; D011877:Radionuclide Imaging; D014018:Tissue Distribution; D005719:gamma-Globulins / Q000493:pharmacokinetics*
D002250:Carbon Radioisotopes; D007074:Immunoglobulin G; D000922:Immunotoxins; C045927:K-18 conjugate; D005719:gamma-Globulins; D008558:Melphalan