Inhibition of DNA strand break rejoining in ultraviolet-irradiated human cells: comparison of aphidicolin and cytosine arabinoside.
When ultraviolet-irradiated mammalian cells are incubated in the presence of aphidicolin or cytosine arabinoside (ara C) DNA breaks accumulate. When the inhibitors are removed before a steady-state level of breaks has been reached, breaks accumulated in the presence of aphidicolin are rapidly sealed while breaks in ara-C-treated cells continue to increase. This suggests that the active metabolite of ara C, ara CTP, cannot, in contrast to aphidicolin, be easily washed out of cells.
Ahnström
G
G
University of Stockholm, Arrhenius Laboratories for Natural Sciences, Department of Radiobiology, Sweden.
D016590:Aphidicolin; D002460:Cell Line; D003561:Cytarabine / Q000494:pharmacology*; D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects*; D004224:Diterpenes / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D013997:Time Factors; D014466:Ultraviolet Rays
D004224:Diterpenes; D003561:Cytarabine; D016590:Aphidicolin; D004247:DNA