title: Chromosome banding analysis by slit-scan flow cytometry.
abstract: We have investigated the use of fluorescence banding patterns for the resolution of metaphase chromosomes by slit-scan flow cytometry. Fluorescence scans of R-banded chromosomes have been obtained for the entire human karyotype. Metaphase chromosomes were R-banded in suspension by staining with chromomycin A3 after hypotonic treatment in Ohnuki's buffer. Specific fluorescent landmark bands were detected for human chromosomes 1-12. Scans obtained for chromosomes 13-22 did not contain sufficient information for classification. Characteristic fluorescence patterns for human chromosomes 1 and 3 provided the clearest evidence for the detection of R-bands by slit-scan flow cytometry. Specific patterns were detected for human chromosomes 9-12 in which the number and placement of the fluorescent bands served as classifiers.
authors.affiliation: Life Sciences Division, Los Alamos National Laboratory, New Mexico 87545.
authors.forename: M F
authors.initials: MF
authors.lastname: Bartholdi
authors.forename: J
authors.initials: J
authors.lastname: Meyne
authors.forename: R G
authors.initials: RG
authors.lastname: Johnston
authors.forename: L S
authors.initials: LS
authors.lastname: Cram
mesh_terms: D002460:Cell Line; D014128:Chromomycin A3; D002871:Chromosome Banding / Q000379:methods*; D002877:Chromosomes, Human / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007621:Karyotyping / Q000379:methods*
chemical_list: D014128:Chromomycin A3