Expression of a human liver cDNA encoding a UDP-glucuronosyltransferase catalysing the glucuronidation of hyodeoxycholic acid in cell culture.
A cDNA encoding a human liver UDPGT (HLUG 25) transcribed and translated in vitro showed that the encoded protein was synthesized as a precursor and was cleaved and glycosylated when dog pancreatic microsomes were present during translation. The UDPGT cDNA was transiently expressed in mammalian cell culture (COS-7 cells) resulting in the biosynthesis of a polypeptide of 52 kDa. This expressed UDPGT glycoprotein catalysed the glucuronidation of hyodeoxycholic acid forming an ether glucuronide. These results suggest that this UDPGT isoenzyme may be responsible for the glucuronidation of 6 alpha-hydroxy bile acids in human liver.
Fournel-Gigleux
S
S
Department of Biochemistry, Medical Sciences Institute, The University, Dundee, Scotland.
Jackson
M R
MR
Wooster
R
R
Burchell
B
B
D000818:Animals; D002384:Catalysis; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D003840:Deoxycholic Acid / Q000378:metabolism*; D014453:Glucuronosyltransferase / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D008099:Liver / Q000201:enzymology*; D008861:Microsomes / Q000201:enzymology; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D013379:Substrate Specificity; D014158:Transcription, Genetic; D014162:Transfection
D003840:Deoxycholic Acid; C010471:hyodeoxycholic acid; D004247:DNA; D014453:Glucuronosyltransferase