[Mechanisms of disruption of DNA in human cells. Mutagenesis of a virus in xeroderma pigmentosum cells as an indicator of a defect in DNA repair].
Lymphocytes of two patients with Xeroderma pigmentosum, their mothers and cell lines isolated from the third patient were examined. The reaction of vaccinia virus treated with mutagens, the index of virus-induced mutagenesis and DNA repair synthesis were the criteria of estimation of cell repair activity. Significant inhibition of DNA repair synthesis was detected in cells of all the patients observed (primary and established lines) in the experiments with UV-irradiation and 4-nitroquinoline-4-oxide. These indexes correlated with decreased virus reactivation and increased level of virus-induced mutagenesis. Inhibition of DNA repair synthesis was also revealed in lymphocytes of both mothers. These data claim a possibility of discovering heterozygotes, the carriers of mutant genes. The data on virus-induced mutagenesis serve as a simple test for detection of DNA repair disorders.
L'vova
G N
GN
Chekova
V V
VV
Prokhorov
A Iu
AIu
Zasukhina
R D
RD
D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology; D002478:Cells, Cultured; D004260:DNA Repair / Q000187:drug effects / Q000528:radiation effects; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000528:radiation effects; D005814:Genes, Viral; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000528:radiation effects; D009154:Mutation; D014466:Ultraviolet Rays; D014616:Vaccinia virus / Q000235:genetics*; D014983:Xeroderma Pigmentosum / Q000235:genetics*
D015112:4-Nitroquinoline-1-oxide
Mekhanizmy narusheniĭ reparatsii DNK v kletkakh cheloveka. Mutagenez virusa v kletkakh pigmentnoĭ kcerodermy kak pokazatel' defekta reparatsii DNK.