title: Studies on the thymus in Chagas' disease. I. Changes in the thymic microenvironment in mice acutely infected with Trypanosoma cruzi.
abstract: Previous observations demonstrated severe thymocyte depletion in mice undergoing acute Chagas' disease. These data led us to investigate the status of the thymic microenvironment in these animals. Young adult C57BL/6 and C3H/HeJ mice were infected i.p. with 10(5) blood-derived trypomastigote forms of Trypanosoma cruzi (CL strain) and killed 7-14 days after infection. Sera were then analyzed for thymic hormone (thymulin) levels, and frozen thymus sections were studied by immunohistochemistry for the expression of functional antigens (thymulin and Ia), the distribution of distinct thymic epithelial cell subsets and extracellular matrix components. Infected mice exhibited a transient decrease in thymulin production and those with severe thymic atrophy showed a denser Ia-bearing cellular network. In addition, an abnormal localization of the TR5 and CK18 antigens restricted to the medullary and cortical TEC subsets, respectively, was observed. Furthermore, an increase in the basement membrane proteins was detected within thymic lobules. We suggest that the thymic microenvironment is also affected during T. cruzi infection, extending the concept that the thymus should be regarded as a target in Chagas' disease.
authors.affiliation: Department of Immunology, Institute Oswaldo Cruz, Rio de Janeiro, Brazil.
authors.forename: W
authors.initials: W
authors.lastname: Savino
authors.forename: M C
authors.initials: MC
authors.lastname: Leite-de-Moraes
authors.forename: M
authors.initials: M
authors.lastname: Hontebeyrie-Joskowicz
authors.forename: M
authors.initials: M
authors.lastname: Dardenne
mesh_terms: D000818:Animals; D001323:Autoantibodies / Q000276:immunology; D014355:Chagas Disease / Q000276:immunology* / Q000473:pathology; D004848:Epithelium / Q000276:immunology / Q000469:parasitology / Q000473:pathology; D005109:Extracellular Matrix / Q000276:immunology / Q000469:parasitology / Q000473:pathology; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D051379:Mice; D013950:Thymus Gland / Q000276:immunology* / Q000473:pathology; D013951:Thymus Hormones / Q000096:biosynthesis; D014349:Trypanosoma cruzi
chemical_list: D001323:Autoantibodies; D000949:Histocompatibility Antigens Class II; D013951:Thymus Hormones