A cell-free model system for the study of antigen processing.
Antigen processing is an essential step in the presentation of most protein antigens to class 2 MHC restricted T cells, but many of the details of processing remain unknown. In this study we show that a whole cell lysate, as well as membrane fractions from an antigen-presenting B cell lymphoma, can process ovalbumin. In this system native ovalbumin incubated with these membranes at acidic pH can be presented to an antigen-specific hybridoma by gluteraldehyde-fixed, antigen-presenting cells. This processing is inhibited by pepstatin, a selective inhibitor of aspartyl proteases. This is the first study of processing by subcellular fractions, and this cell-free system will provide a good model in which to dissect further the molecular requirements of antigen processing.
Shaw
M A
MA
Department of Biology, University College London, UK.
Chain
B M
BM
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D000941:Antigens / Q000378:metabolism*; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism; D002474:Cell-Free System / Q000276:immunology / Q000378:metabolism; D008954:Models, Biological; D010047:Ovalbumin / Q000276:immunology / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D013347:Subcellular Fractions / Q000276:immunology / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000276:immunology / Q000378:metabolism
D000941:Antigens; D010047:Ovalbumin; D010447:Peptide Hydrolases