Biochemical analysis of bovine lens sections obtained by a new sectioning device.
One mm thick layers were reproducibly sliced from the punched-out inner cylinder of bovine lenses. The layers were obtained by a special designed freeze-sectioning device. Biochemical analysis of these layers revealed a detailed picture of the lenticular distribution of the amount of water-insoluble and urea-insoluble fractions. Furthermore, the distribution of the content of glucose, fructose and sorbitol was established. The results clearly show the usefulness of a microsectioning device to obtain layers for subsequent biochemical analysis which may lead to a comprehensive understanding of age- and/or cataract-related alterations within one lens.
Bessems
G J
GJ
Department of experimental Ophthalmology, University of Bonn, FRG.
Dragomirescu
V
V
Möller
B
B
Korte
I
I
Hockwin
O
O
D000375:Aging / Q000378:metabolism; D000818:Animals; D002417:Cattle; D003459:Crystallins / Q000378:metabolism; D004867:Equipment Design; D005629:Frozen Sections; D005632:Fructose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D007904:Lens Cortex, Crystalline / Q000378:metabolism*; D008867:Microtomy / Q000295:instrumentation*; D012995:Solubility; D013012:Sorbitol / Q000378:metabolism
D003459:Crystallins; D005632:Fructose; D013012:Sorbitol; D005947:Glucose