title: Effect of donation time on platelet concentrates and fresh-frozen plasma. An in vitro study.
abstract: To investigate the effect of donation time on the quality of blood components, we measured the platelet count and pH on platelet concentrates, and the factor V and VIII:C levels and fibrinopeptide A concentration on fresh-frozen plasma by duration of donation time. Platelet concentrates and fresh-frozen plasma were classified into three groups according to donation time: group 1, less than 10 min; group 2, 10-15 min, and group 3, longer than 15 min. Mean platelet counts of platelet concentrate were: group 1, 8.6 +/- 2.5 (in x 10(10], group 2, 8.1 +/- 2.6, and group 3, 6.5 +/- 3.2 (p less than 0.05). The same pH was maintained in all three groups. The fibrinopeptide A concentrations in groups 1 and 2 were 24 +/- 53 and 169 +/- 64 ng/ml, respectively, while in group 3 all were greater than 200 ng/ml, indicating correlation of a higher fibrinopeptide A level with longer donation time. Although a higher fibrinopeptide A level indicated a greater degree of thrombin generation, assays of factors V and VII:C did not show decreased activity in groups 2 and 3.
authors.affiliation: University of Texas, M.D. Anderson Cancer Center at Houston.
authors.forename: Y O
authors.initials: YO
authors.lastname: Huh
authors.forename: B
authors.initials: B
authors.lastname: Lichtiger
authors.forename: G G
authors.initials: GG
authors.lastname: Giacco
authors.forename: V F
authors.initials: VF
authors.lastname: Guinee
authors.forename: B
authors.initials: B
authors.lastname: Drewinko
mesh_terms: D000941:Antigens / Q000032:analysis; D001792:Blood Platelets; D001800:Blood Specimen Collection; D005165:Factor V / Q000032:analysis; D005169:Factor VIII / Q000032:analysis; D005344:Fibrinopeptide A / Q000032:analysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010949:Plasma; D010976:Platelet Count; D013997:Time Factors
chemical_list: D000941:Antigens; D005344:Fibrinopeptide A; D005165:Factor V; D005169:Factor VIII