title: In vitro and in vivo interaction between sporocysts of Sarcocystis muris and mouse peritoneal macrophages.
abstract: The interaction between the sporocysts of Sarcocystis muris and mouse peritoneal macrophages was studied both in vitro and in vivo in an attempt to determine whether or not resident peritoneal macrophages might effect the excystation of S. muris sporozoites from sporocysts injected intraperitoneally. Sporocysts of S. muris were phagocytosed by peritoneal macrophages both in vitro and in vivo. The addition of either unheated mouse serum or fetal calf serum did not significantly alter the level of phagocytosis. The percentage of phagocytosis in vivo and by thioglycolate-, proteose peptone- and BCG-elicited macrophages in vitro was greater than that shown by unstimulated macrophages in vitro. After 8 h incubation in vivo and in vitro a small proportion of sporocysts (less than 5%) was seen to have collapsed walls and up to 5% to have stained sporozoites, suggesting increased permeability of the sporocyst wall. The significance of increased permeability of the cyst wall in the process of sporozoite excystation is discussed.
authors.affiliation: Department of Veterinary Pathology and Public Health, Massey University, Palmerston North, New Zealand.
authors.forename: H S
authors.initials: HS
authors.lastname: Gill
authors.forename: K M
authors.initials: KM
authors.lastname: Moriarty
authors.forename: W A
authors.initials: WA
authors.lastname: Charleston
mesh_terms: D000818:Animals; D008264:Macrophages / Q000276:immunology* / Q000469:parasitology / Q000648:ultrastructure; D051379:Mice; D010529:Peritoneal Cavity / Q000166:cytology; D010587:Phagocytosis; D012522:Sarcocystis / Q000276:immunology*; D012523:Sarcocystosis / Q000276:immunology / Q000469:parasitology*