The 5S RNA gene minichromosome of Euplotes.
The macronucleus of the ciliated protozoan Euplotes eurystomus contains about 10(6) copies of a single type of 5S ribosomal RNA gene. This 5S gene DNA is only 930 bp long, is flanked by telomeres, and contains a single coding region of 120 bp which serves as a template for transcription in vivo and in vitro. The 5S gene minichromatin possesses four positioned nucleosomes and hypersensitive cleavage sites in the telomeric regions.
Roberson
A E
AE
U.T.-Oak Ridge Graduate School of Biomedical Sciences, Biology Division, TN 37831-8077.
Wolffe
A P
AP
Hauser
L J
LJ
Olins
D E
DE
D000818:Animals; D001483:Base Sequence; D002843:Chromatin / Q000235:genetics / Q000302:isolation & purification; D002875:Chromosomes / Q000032:analysis* / Q000378:metabolism; D016798:Ciliophora / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D012335:RNA, Ribosomal / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
D002843:Chromatin; D012335:RNA, Ribosomal; D012341:RNA, Ribosomal, 5S