title: Mutations at six additional loci of Drosophila melanogaster cause alkylation hypermutability.
abstract: 8 mutagen-sensitive strains of Drosophila melanogaster were examined for their effects on alkylation-induced mutagenesis. Using methylnitrosourea as the DNA-damaging agent and the sex-linked recessive lethal test as the monitor of genetic endpoint, 6 of these strains were shown to be hypermutable following exposure to this alkylating agent. Previous studies of 6 other genes have demonstrated that strains exhibiting alkylation hypermutability are completely defective in repair replication following alkylation-induced DNA damage. The present observations suggest that at least 12 loci may be required for excision repair of alkylation DNA damage in this species.
authors.affiliation: Department of Biological Sciences, Southern Methodist University, Dallas, TX 75275.
authors.forename: P D
authors.initials: PD
authors.lastname: Smith
authors.forename: R L
authors.initials: RL
authors.lastname: Dusenbery
mesh_terms: D000477:Alkylating Agents / Q000633:toxicity; D000478:Alkylation; D000818:Animals; D002874:Chromosome Mapping; D004249:DNA Damage; D004260:DNA Repair; D004331:Drosophila melanogaster / Q000235:genetics*; D008770:Methylnitrosourea / Q000633:toxicity; D009152:Mutagenicity Tests; D009154:Mutation
chemical_list: D000477:Alkylating Agents; D008770:Methylnitrosourea