Submicrogram quantities of unstained proteins are visualized on polyvinylidene difluoride membranes by transillumination.
Unstained proteins on polyvinylidene difluoride membranes which have been dried and saturated with 20% methanol appear as clear areas when placed on a white light box. Sensitivity is comparable to that obtained with Coomassie Blue R250. This procedure greatly reduces the potential for unintended chemical modification during staining procedures, provides a nondestructive means of determining the major features of a protein pattern on a blot and makes it possible to initiate sequencing within minutes after blotting is complete.
Reig
J A
JA
Section on Neuroendocrinology, National Institute of Child Health and Human Development, National Institute of Health, Bethesda, Maryland 20892.
Klein
D C
DC
D008567:Membranes, Artificial; D008832:Microchemistry; D011145:Polyvinyls; D011506:Proteins / Q000032:analysis*; D013194:Staining and Labeling; D014172:Transillumination; D005465:Fluorocarbon Polymers
D008567:Membranes, Artificial; D011145:Polyvinyls; D011506:Proteins; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers