[Cytotoxicity of dental iodine glycerin on cultured mammalian cells].
Cytotoxicity of dental iodine glycerin was studied using cultured Chinese hamster lung cells (V79 cells). Treatment with dental iodine glycerin at 0.33 - 0.1v/v% for 24 to 72 hr elicited a minor inhibition of growth of V79 cells. The cell growth, however, was remarkably inhibited when the cells were treated with dental iodine glycerin at 0.3v/v%. No inhibitory effect on survival of V79 cells was observed in cultures treated with dental iodine glycerin at 0.03 - 0.1v/v% for 2 to 24 hr. Meanwhile, treatment with dental iodine glycerin at 0.3v/v% for 2 hr or more induced 100% inhibition of cell survival. Exposure of cells to dental iodine glycerin at 0.3v.v% for 2 hr caused decrease in DNA, RNA or protein synthesis of the cells up to 54.4% of untreated cells. These results indicate that there is a big difference in cytotoxicity induced by treatment of V79 cells with dental iodine glycerin at concentrations between 0.1v/v% and 0.3v.v%, and that treatment with dental iodine glycerin induced inhibition of DNA, RNA and protein synthesis of the cells and the inhibition occurs even by the treatment which causes no reduction of cell survival.
Ohyama
M
M
Bae
R D
RD
Fujiwara
H
H
Ushimura
H
H
Ushimura
A
A
Tsutsui
T
T
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000096:biosynthesis; D005990:Glycerol / Q000031:analogs & derivatives; D007455:Iodine / Q000633:toxicity*; D008168:Lung / Q000166:cytology; D011500:Protein Synthesis Inhibitors / Q000633:toxicity*; D012313:RNA / Q000096:biosynthesis
D011500:Protein Synthesis Inhibitors; D012313:RNA; D004247:DNA; D007455:Iodine; D005990:Glycerol