title: Analysis of the sporogonic development of Plasmodium falciparum and Plasmodium berghei in anopheline mosquitoes.
abstract: Basic knowledge of the sporogonic development of malarial parasites is crucial when evaluating the sporontocidal activity of antimalarial drugs or when determining why certain vectors are refractory to a particular parasite while others are competent vectors. We have developed a model which we have used to i) assess the sporogonic development of Plasmodium berghei ANKA in Anopheles stephensi and A. freeborni mosquitoes and ii) determine the effect of chloroquine on the sporogony of P. falciparum NF-54 in A. stephensi. Criteria used to assay sporogonic development include: i) number of oocysts present, ii) percentage of mosquitoes with oocysts, iii) time of release of sporozoites from the oocysts into the hemolymph, iv) time and degree of sporozoite invasion of salivary glands, and v) transmission (P. berghei) into vertebrate hosts. Parasite development in the mosquito is evaluated every other day, commencing on ca. day 7 post-feed (PF) and continuing until ca. day 22 PF. These detailed observations allow us to delineate the chronology of sporogonic development.
authors.affiliation: Biomedical Research Institute, Rockville, MD 20852.
authors.forename: V E
authors.initials: VE
authors.lastname: Do Rosario
authors.forename: J A
authors.initials: JA
authors.lastname: Vaughan
authors.forename: R E
authors.initials: RE
authors.lastname: Coleman
mesh_terms: D000818:Animals; D000852:Anopheles / Q000469:parasitology*; D002738:Chloroquine; D005260:Female; D006458:Hemolymph / Q000469:parasitology; D007814:Larva; D051379:Mice; D010962:Plasmodium berghei / Q000254:growth & development*; D010963:Plasmodium falciparum / Q000187:drug effects / Q000254:growth & development*; D012469:Salivary Glands / Q000469:parasitology; D013045:Species Specificity
chemical_list: D002738:Chloroquine