title: Characteristic mode of action of gangliosides in selective modulation of CD4 on human T lymphocytes.
abstract: We have explored the possible mechanisms for selective modulation by gangliosides of CD4 on human T lymphocytes and subsequent re-expression of CD4. Indirect immunofluorescence staining with anti-CD4 antibodies revealed newly internalized CD4 in ganglioside-treated cells after membrane permeabilization with 0.1% saponin. Cycloheximide and other metabolic inhibitors did not alter the modulation but inhibited significantly the re-expression of CD4. These results suggest both selective modulation of CD4 by a process of endocytosis and re-expression of CD4 through de novo protein synthesis.
authors.affiliation: Second Department of Internal Medicine, Kumamoto University Medical School, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kawaguchi
authors.forename: H
authors.initials: H
authors.lastname: Nakakuma
authors.forename: T
authors.initials: T
authors.lastname: Kagimoto
authors.forename: K
authors.initials: K
authors.lastname: Shirono
authors.forename: K
authors.initials: K
authors.lastname: Horikawa
authors.forename: M
authors.initials: M
authors.lastname: Hidaka
authors.forename: M
authors.initials: M
authors.lastname: Iwamori
authors.forename: Y
authors.initials: Y
authors.lastname: Nagai
authors.forename: K
authors.initials: K
authors.lastname: Takatsuki
mesh_terms: D000945:Antigens, Differentiation, T-Lymphocyte; D015153:Blotting, Western; D002462:Cell Membrane / Q000276:immunology; D002463:Cell Membrane Permeability / Q000187:drug effects; D002772:Cholera Toxin; D004305:Dose-Response Relationship, Drug; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D005677:G(M1) Ganglioside / Q000494:pharmacology; D005732:Gangliosides / Q000494:pharmacology*; D006801:Humans; D015458:Leukemia, T-Cell; D012503:Saponins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D013861:Thiocyanates; D014407:Tumor Cells, Cultured
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D005452:Fluoresceins; D005456:Fluorescent Dyes; D005732:Gangliosides; D012503:Saponins; D013861:Thiocyanates; D005677:G(M1) Ganglioside; D002772:Cholera Toxin; D016650:Fluorescein-5-isothiocyanate