title: Effects of bile salts on energy metabolism and acid secretion by the isolated toad gastric mucosa.
abstract: The effects of bile salts on energy metabolism and acid secretion were investigated in the amphibian gastric mucosa in vitro. Serosal exposure to deoxycholate (0.2-2 mM), cholate (2-8 mM), or taurocholate (4-80 mM), at pH 7.4, decreased acid secretion depending on the concentration and time of exposure. Mucosal application of cholate and taurocholate at pH 5.6 caused a more pronounced reduction in the apparent rate of acid secretion. Oxygen uptake and substrate oxidations were significantly inhibited by bile salts in a dose-dependent manner. At pH 7.4, 1 mM deoxycholate inhibited the respiration by 49% and the rates of oxidation by 51%, 52%, 78%, 74%, and 54% of control values, for glucose, pyruvate, succinate, acetate, and butyrate, respectively. Cholate and taurocholate were found to be less potent than deoxycholate. Tissue adenosine triphosphate concentration was decreased by 13%, 57%, and 67% with 0.5, 1, and 2 mM deoxycholate, respectively. We believe the impairment of energy metabolism could be involved in the mechanism of bile salt injury to the gastric mucosa.
authors.affiliation: Instituto de Investigaciones Biológicas, Facultad de Medicina, Universidad del Zulia, Maracaibo, Venezuela.
authors.forename: D
authors.initials: D
authors.lastname: Núñez
authors.forename: J
authors.initials: J
authors.lastname: Chacín
mesh_terms: D000818:Animals; D001647:Bile Acids and Salts / Q000494:pharmacology*; D002024:Bufo marinus; D019826:Cholic Acid; D002793:Cholic Acids / Q000494:pharmacology; D003840:Deoxycholic Acid / Q000494:pharmacology; D004734:Energy Metabolism; D005744:Gastric Acid / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism*; D066298:In Vitro Techniques; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D012636:Secretory Rate; D013656:Taurocholic Acid / Q000494:pharmacology
chemical_list: D001647:Bile Acids and Salts; D002793:Cholic Acids; D003840:Deoxycholic Acid; D013656:Taurocholic Acid; D019826:Cholic Acid