Screening for selected trace elements in single cell populations by energy dispersive X-ray fluorescence spectrometry.
An adaptation of the thin film approach to energy dispersive X-ray fluorescence spectrometry (EDXRF) is described, which provides for a screening analysis for Fe, Ni, Cu, Zn, Se, and Cd in K-562 leukemic cells incubated in various metal-enhanced cultures. Valuable quantitative and semiquantitative data can be obtained on as few as 400,000 cells.
Bray
J T
JT
Elemental Analytical Group/Shared Research Resources Laboratories, School of Medicine, East Carolina University, Greenville, NC 27858.
Maier
R H
RH
Pories
W J
WJ
D002478:Cells, Cultured; D008567:Membranes, Artificial; D012015:Reference Standards; D013052:Spectrometry, X-Ray Emission; D014131:Trace Elements / Q000032:analysis*
D008567:Membranes, Artificial; D014131:Trace Elements