A novel germ-line JK transcript starting immediately upstream of JK1.
Germ-line transcripts of the immunoglobulin (Ig) and T cell receptor loci are thought to be involved in the control of V gene rearrangement by rendering these loci accessible to the recombinases. We have analyzed the transcriptional activity of germ-line K alleles in two bone marrow-derived Abelson-murine leukemia virus transformed pre-B cells: 300-19, a null cell line, and P8 a mu-producing line. We found a novel germ-line JK transcript starting immediately upstream of JK1 and spliced to CK. The potential role of this transcript in the opening of the Ig K locus as well as in the ordered usage of JK segments is discussed.
Leclercq
L
L
Institute of Genetics, University of Cologne, Köln, FRG.
Butkeraitis
P
P
Reth
M
M
D000818:Animals; D001402:B-Lymphocytes / Q000032:analysis; D001483:Base Sequence; D015328:Gene Rearrangement, B-Lymphocyte, Light Chain; D005803:Genes, Immunoglobulin; D006801:Humans; D007127:Immunoglobulin Constant Regions / Q000235:genetics; D007144:Immunoglobulin J-Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D007127:Immunoglobulin Constant Regions; D007144:Immunoglobulin J-Chains; D007135:Immunoglobulin Variable Region; D007145:Immunoglobulin kappa-Chains