Variability in electrophoretic mobility of Gi-like proteins: effect of SDS.
Two forms of Gi-like protein are resolved in both somatic cells and mouse gametes when Sigma SDS (95% grade) is used during polyacrylamide gel electrophoresis, whereas only a single species is resolved when Bio-Rad SDS (electrophoresis grade) is used. These two Gi-like proteins are likely to reflect two distinct species, since (i) the two species resolved in the presence of Sigma SDS migrate with the same electrophoretic mobility upon re-electrophoresis in the presence of Sigma SDS and (ii) exchanging Sigma SDS for Bio-Rad SDS resolves a single species, whereas exchanging Bio-Rad SDS for Sigma SDS resolves two species.
Jones
J
J
Department of Biology, University of Pennsylvania, Philadelphia 19104.
Kopf
G S
GS
Schultz
R M
RM
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000818:Animals; D001345:Autoradiography; D002384:Catalysis; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005260:Female; D019204:GTP-Binding Proteins / Q000032:analysis*; D008297:Male; D051379:Mice; D010063:Ovum / Q000032:analysis; D012967:Sodium Dodecyl Sulfate / Q000592:standards; D013094:Spermatozoa / Q000032:analysis; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D010566:Virulence Factors, Bordetella; D000246:Adenosine Diphosphate Ribose; D012967:Sodium Dodecyl Sulfate; C028913:dodecyl sulfate; D019204:GTP-Binding Proteins