title: Dopa in plasma increases during acute exercise and after exercise training.
abstract: Plasma dihydroxyphenylalanine (dopa) has been shown to originate in sympathetic neurons, and it has been suggested that plasma level reflects activity of tyrosine hydroxylase, the rate-limiting enzyme in the synthesis of catecholamines. In this study, we measured the effects of acute exercise and exercise training on the levels of dopa and catecholamines in the plasma of healthy, older individuals. Venous blood was drawn from 19 men, from 52 to 75 years of age, at rest, at a standard submaximal work load, at peak exercise, and 3 minutes after exercise on a cycle ergometer. Ten of 12 men then completed 12 to 16 weeks of supervised training, and seven continued normal activity. All 17 men were then retested. The seven control subjects subsequently underwent exercise training as above and were retested again. Levels of dopa and catecholamines in plasma samples were measured by high-performance liquid chromatography with electrochemical detection. Dopa levels at rest were considerably higher than free dopamine, epinephrine, and norepinephrine. During short-term exercise, levels of dopa and catecholamines increased. The absolute increase in dopa was greater than the increase in epinephrine or dopamine but was not greater than that in norepinephrine. After the training period, basal dopa levels increased significantly and correlated with the increase in peak oxygen uptake. There was no change in basal conjugated norepinephrine or dopamine levels with exercise or training, but the level of conjugated epinephrine decreased slightly. No changes occurred in levels of dopa or catecholamines in the untrained group. Free dopamine, norepinephrine, and epinephrine levels at peak exercise were increased after exercise training.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Division of Cardiovascular Diseases and Internal Medicine, Mayo Clinic, Rochester, MN 55905.
authors.forename: M L
authors.initials: ML
authors.lastname: Devalon
authors.forename: T D
authors.initials: TD
authors.lastname: Miller
authors.forename: R W
authors.initials: RW
authors.lastname: Squires
authors.forename: P J
authors.initials: PJ
authors.lastname: Rogers
authors.forename: A A
authors.initials: AA
authors.lastname: Bove
authors.forename: G M
authors.initials: GM
authors.lastname: Tyce
mesh_terms: D001800:Blood Specimen Collection; D004295:Dihydroxyphenylalanine / Q000097:blood*; D015444:Exercise; D006801:Humans; D008297:Male; D008875:Middle Aged; D009638:Norepinephrine / Q000097:blood; D005082:Physical Exertion
chemical_list: D004295:Dihydroxyphenylalanine; D009638:Norepinephrine