Gas chromatographic analysis of norcantharidin and related compounds using derivatization to imides.
Derivatization to imides has been studied for determining alpha,beta-diacids or corresponding anhydrides (e.g. norcantharidin) as well as for analysing primary amines by gas chromatography. 2-Naphthylaniline, n-heptylamine, cyclohexamine, 2,5-dimethoxyaniline, 2,4,5-trichloroaniline, 3,5-dichloroaniline, aniline and benzylaniline were used as derivatization reagents. 1H-nuclear magnetic resonance and mass spectrometry data were obtained for the norcantharidin N-imides synthesized. Factors such as derivatization yields, linearity, reproducibility and sensitivity were evaluated. The influences of reaction temperature and solvent were investigated.
Li
Q M
QM
Department of Analytical Chemistry, Beijing Medical University, P.R. China.
Dong
S N
SN
D000818:Animals; D001643:Bridged Bicyclo Compounds / Q000032:analysis*; D019086:Bridged Bicyclo Compounds, Heterocyclic; D002849:Chromatography, Gas; D007094:Imides / Q000032:analysis / Q000138:chemical synthesis*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D011817:Rabbits; D012997:Solvents; D013696:Temperature
D001643:Bridged Bicyclo Compounds; D019086:Bridged Bicyclo Compounds, Heterocyclic; D007094:Imides; D012997:Solvents; C069741:norcantharidin