title: High-performance liquid chromatography for the determination of three new fluoroquinolones, fleroxacin, temafloxacin and A-64730, in biological fluids.
abstract: High-performance liquid chromatographic procedures have been developed for the measurement of three new fluoroquinolones, fleroxacin, temafloxacin and A-64730, in serum, urine and bile. The sample treatment consists of a two-step chemical extraction. The three molecules are chromatographed on a C18 reversed-phase analytical column with spectrofluorimetric detection. At a signal-to-noise ratio of 4, the detection limits in serum are 2.5, 10 and 20 ng/ml, for fleroxacin, temafloxacin and A-64730, respectively. The calibration curves are rectilinear between these detection limits and 20 micrograms/ml. The intra- and inter-assay coefficients of variation are in the ranges 0.8-5.4 and 2.2-7.6%, respectively. These simple and reliable assay procedures will be of great interest for further pharmacokinetic studies and drug monitoring in hospital use.
authors.affiliation: Institute of Bacteriology, Faculty of Medicine, Strasbourg, France.
authors.forename: C
authors.initials: C
authors.lastname: Koechlin
authors.forename: F
authors.initials: F
authors.lastname: Jehl
authors.forename: L
authors.initials: L
authors.lastname: Linger
authors.forename: H
authors.initials: H
authors.lastname: Monteil
mesh_terms: D000890:Anti-Infective Agents / Q000032:analysis* / Q000097:blood / Q000652:urine; D001646:Bile / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D002939:Ciprofloxacin / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000652:urine; D016576:Fleroxacin; D024841:Fluoroquinolones; D006801:Humans; D015394:Molecular Structure; D009287:Naphthyridines; D015363:Quinolones; D013050:Spectrometry, Fluorescence
chemical_list: D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D009287:Naphthyridines; D015363:Quinolones; C054745:temafloxacin; D002939:Ciprofloxacin; C055185:tosufloxacin; D016576:Fleroxacin