Zinc inhibits pokeweed mitogen-induced development of immunoglobulin-secreting cells through augmentation of both CD4 and CD8 cells.
The mechanism whereby zinc regulates the in vitro antibody synthesis of human peripheral blood mononuclear cells was investigated. In a serum-free culture, zinc inhibited the pokeweed mitogen (PWM)-induced generation of immunoglobulin secreting cells (ISC) through an apparent non-specific augmentation of T-cells, which included CD4 and CD8 cells, as well as both CD8-Leu8+ and CD8-Leu8- cells. It was noted that CD4 cells were more stimulated than CD8 cells, and that CD8-Leu8- cells were more stimulated than CD8 Leu8+ cells. CD8-Leu8- cells inhibited the development of ISC. These findings indicate that, although zinc stimulates all T-cells, the relative potency of zinc differs among T-cell subsets; helper inducer T-cells appear to be most strongly stimulated.
Tanaka
Y
Y
Department of Medicine, Kobe University School of Medicine, Japan.
Shiozawa
S
S
Morimoto
I
I
Fujita
T
T
D000921:Antibody-Producing Cells / Q000187:drug effects* / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D015704:CD4 Antigens / Q000276:immunology*; D016827:CD8 Antigens; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D007202:Indicators and Reagents; D009000:Monocytes / Q000187:drug effects / Q000276:immunology*; D011043:Pokeweed Mitogens / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D015032:Zinc / Q000494:pharmacology*
D000945:Antigens, Differentiation, T-Lymphocyte; D015704:CD4 Antigens; D016827:CD8 Antigens; D007202:Indicators and Reagents; D011043:Pokeweed Mitogens; D015032:Zinc