Complexing of copper in drinking water samples to enhance recovery of Aeromonas and other bacteria.
The presence of copper in drinking water samples at concentrations as low as 10 micrograms/l resulted in a rapid die-off of aeromonads. Coliform bacteria, heterotrophic plate count bacteria and faecal streptococci were also sensitive to copper but to a lesser degree than aeromonads. The effect was particularly noticeable in soft water (less than 3 meq/l Ca + Mg) and at pH-values below 8.0. The toxic effect of copper concentrations up to 500 micrograms/l could be neutralized for a period of up to 24 h by the addition to samples of 50 mg/l of disodium-ethylene-diamino-tetraacetate (Na2EDTA) and keeping the samples on melting ice.
Versteegh
J F
JF
National Institute of Public Health and Environmental Protection, The Hague, The Netherlands.
Havelaar
A H
AH
Hoekstra
A C
AC
Visser
A
A
D000333:Aeromonas / Q000187:drug effects* / Q000254:growth & development; D015169:Colony Count, Microbial; D003300:Copper / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000254:growth & development; D006863:Hydrogen-Ion Concentration; D009426:Netherlands; D013291:Streptococcus / Q000187:drug effects* / Q000254:growth & development; D014871:Water Microbiology; D014881:Water Supply
D003300:Copper; D004492:Edetic Acid