Induction of testicular DNA synthesis in vitro by hCG and hMG treatments in men with hypogonadotropic hypogonadism.
To study the effectiveness of hCG and hMG administration on spermatogenesis of patients with hypogonadotropic hypogonadism (HH), the DNA synthesis of spermatogonia was investigated by the method of 3H-thymidine uptake by cultured tissue fragments of human testis with or without treatment with hCG and/or hMG. Although in two groups treated with different concentrations of hCG the value of 3H-thymidine incorporation into testicular tissue showed no significant difference compared with the untreated control group, treatment with both hCG and hMG induced significant changes in the value of 3H-thymidine incorporation compared with the untreated control group. Combined hCG and hMG therapy was effective for induction of spermatogenesis in patients with HH.
Okuyama
A
A
Department of Urology and Pathology, Osaka University Medical School, Japan.
Nonomura
N
N
Koh
E
E
Kondoh
N
N
Nakamura
M
M
Namiki
M
M
Kiyohara
H
H
Nishimune
Y
Y
Matsumoto
K
K
Matsuda
M
M
D000293:Adolescent; D000328:Adult; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D046508:Culture Techniques; D004247:DNA / Q000096:biosynthesis*; D004338:Drug Combinations; D005640:Follicle Stimulating Hormone / Q000097:blood; D006801:Humans; D007006:Hypogonadism / Q000188:drug therapy / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D008596:Menotropins / Q000494:pharmacology*; D013091:Spermatogenesis; D013737:Testis / Q000378:metabolism*; D013739:Testosterone / Q000097:blood; D013936:Thymidine / Q000378:metabolism
D006063:Chorionic Gonadotropin; D004338:Drug Combinations; D013739:Testosterone; D008596:Menotropins; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D004247:DNA; D013936:Thymidine