[Combined use of rose Bengal and indirect immunofluorescence in the diagnosis of brucellosis].
In the present prospective study we have evaluated the sensitivity, specificity and predictive value of the Bengal rose and indirect immunofluorescence (IIF) in 122 patients with a bacteriological diagnosis of brucellosis. The sensitivity of the Bengal rose was 95.79% and its specificity 98.43%. IIF had a sensitivity of 68.80% and a specificity of 100% when the polyvalent anti-IgS conjugate was used. The combined parallel use of Bengal rose and IIF-IgS resulted in a sensitivity of 89.17% and a specificity of 100%. There was a good correlation between Bengal rose and serum agglutination test (r = 0.72); on the contrary, there were not good correlations between the fluorescent conjugates and Bengal rose, agglutination and Coombs test. In conclusion, the combined use of Bengal rose as a screening test and IIF as a confirmation study is a good diagnostic strategy for human brucellosis.
Colmenero
J D
JD
Reguera
J M
JM
Cabrera
F P
FP
Hernández
S
S
Porras
J
J
Manchado
P
P
Miranda
M T
MT
D000372:Agglutination Tests; D000907:Antibodies, Bacterial / Q000032:analysis; D002002:Brucella / Q000276:immunology; D002006:Brucellosis / Q000175:diagnosis*; D005455:Fluorescent Antibody Technique; D006801:Humans; D008403:Mass Screening; D012395:Rose Bengal; D012680:Sensitivity and Specificity
D000907:Antibodies, Bacterial; D012395:Rose Bengal
Empleo combinado del rosa de Bengala e inmunofluorescencia indirecta en el diagnóstico de la brucelosis.