title: Experimental replantation of canine forelimbs after 78.5 hours of anoxia.
abstract: To assess the effect of ischemia on osteocyte survival and longitudinal growth in bone, one forelimb of eight puppies and seven dogs was amputated, perfused with iced Collins solution, maintained at 4 degrees C for 72 hours (78.5 hours total anoxia), and replanted. Five puppies were kept for 1 year to assess bone growth. Undergoing bone labeling on days 1, 8, and 15, the other animals were sacrificed at 22 days to assess osteocyte survival. Osteocytes survived replantation in all dogs and one puppy; most osteocytes died in two puppies. In five long-term puppies, central epiphyseal growth was disturbed, but the peripheral portions maintained nearly normal growth, with almost normal bone length being achieved at 1 year.
authors.affiliation: Christine M. Kleinert Institute for Hand and Micro Surgery, Louisville, Ky.
authors.forename: M
authors.initials: M
authors.lastname: Beppu
authors.forename: F
authors.initials: F
authors.lastname: Takahashi
authors.forename: T M
authors.initials: TM
authors.lastname: Tsai
authors.forename: L
authors.initials: L
authors.lastname: Ogden
authors.forename: J B
authors.initials: JB
authors.lastname: Sharp
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000098:blood supply / Q000473:pathology; D004285:Dogs; D005552:Forelimb / Q000098:blood supply / Q000254:growth & development / Q000473:pathology / Q000601:surgery*; D005684:Gait; D006132:Growth Plate / Q000098:blood supply / Q000473:pathology; D007511:Ischemia / Q000503:physiopathology*; D009133:Muscular Atrophy / Q000209:etiology; D010011:Osteocytes / Q000166:cytology; D010020:Osteonecrosis / Q000473:pathology; D011859:Radiography; D011884:Radius / Q000000981:diagnostic imaging / Q000254:growth & development; D012092:Replantation / Q000009:adverse effects; D013997:Time Factors; D014457:Ulna / Q000000981:diagnostic imaging / Q000254:growth & development