Bovine islets of Langerhans. Potential source for transplantation?
Two methods are described for large-scale isolation of islets from the bovine pancreas. With the single-endpoint technique the average number of islets isolated from a 73.3-g pancreas sample was 150,560, representing an estimated total islet volume of 0.391 ml. Pancreases processed by the continuous digestion-filtration method yielded an average of 246,229 islets from a 93.9-g pancreas sample, representing an estimated total islet volume of 0.458 ml. Isolated bovine pancreatic islets demonstrated a well-preserved endocrine cell ultrastructure, responded to acute stimulation with propionic acid, and temporarily reversed experimental diabetes in mice.
Hering
B J
BJ
Medical Clinic III and Policlinic, Justus Liebig University, Giessen, Federal Republic of Germany.
Romann
D
D
Clarius
A
A
Brendel
M
M
Slijepcevic
M
M
Bretzel
R G
RG
Federlin
K
K
D000818:Animals; D002417:Cattle; D002469:Cell Separation / Q000379:methods*; D016381:Islets of Langerhans Transplantation; D011422:Propionates / Q000494:pharmacology; D014183:Transplantation, Heterologous
D011422:Propionates; C029658:propionic acid