Reversal of impaired wound healing in irradiated rats by platelet-derived growth factor-BB.
This study examined the potential influence of platelet-derived growth factor-BB homodimers (PDGF-BB) on surgical incisions in irradiated animals with depressed wound healing. Rats were irradiated with either 800 rads total body or 2,500 rads surface irradiation. Parallel dorsal skin incisions were made 2 days later, and PDGF-BB was applied topically a single time to one of two incisions. In total body-irradiated rats, bone marrow-derived elements were severely depressed, wound macrophages were virtually eliminated, and PDGF-BB treatment was ineffective. However, in surface-irradiated rats, PDGF-BB treatment recruited macrophages into wounds and partially reversed impaired healing on day 7 (p less than 0.005) and day 12 (p less than 0.001). PDGF-BB-treated wounds were 50 percent stronger than the paired control wounds. The results suggest PDGF requires bone marrow-derived cells, likely wound macrophages, for activity and that it may be useful as a topical agent in postirradiation surgical incisions.
Mustoe
T A
TA
Department of Surgery, Washington University Medical Center, St. Louis, Missouri.
Purdy
J
J
Gramates
P
P
Deuel
T F
TF
Thomason
A
A
Pierce
G F
GF
D000818:Animals; D001853:Bone Marrow / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000502:physiology; D008264:Macrophages / Q000528:radiation effects; D008297:Male; D010982:Platelet-Derived Growth Factor / Q000627:therapeutic use*; D011882:Radiotherapy, High-Energy / Q000009:adverse effects*; D051381:Rats; D011919:Rats, Inbred Strains; D012867:Skin / Q000528:radiation effects; D014916:Whole-Body Irradiation / Q000009:adverse effects; D014945:Wound Healing / Q000528:radiation effects*
D010982:Platelet-Derived Growth Factor