Determination of the thalidomide analogues 2-(2,6-dioxopiperidine-3-yl)phthalimidine (EM 12), 2-(2,6-dioxopiperidine-4-yl)phthalimidine (EM 16) and their metabolites in biological samples.
A rapid and sensitive method for quantitative analysis of the thalidomide analogues EM 12 and EM 16 and their metabolites has been developed. Following an optional extraction, samples were analysed by reversed-phase high-performance liquid chromatography with ion depression. The recovery of the extraction procedures was 65-80%. The method has been applied to pharmacokinetic studies in small laboratory animals and in vitro model experiments.
Winckler
K
K
Freie Universität Berlin, Institut für Biophysik, F.R.G.
Klinkmüller
K D
KD
Schmahl
H J
HJ
D000818:Animals; D002143:Callitrichinae; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D051381:Rats; D011919:Rats, Inbred Strains; D013056:Spectrophotometry, Ultraviolet; D013792:Thalidomide / Q000031:analogs & derivatives* / Q000032:analysis* / Q000097:blood / Q000378:metabolism / Q000493:pharmacokinetics / Q000652:urine
C001079:2-(2,6-dioxopiperidin-3-yl)phthalimidine; C060329:EM 16; D013792:Thalidomide