Western blot (immunoblot) assay of small, round-structured virus associated with an acute gastroenteritis outbreak in Tokyo.
Small, round-structured virus (SRSV) was detected in a stool specimen of a patient during an acute gastroenteritis outbreak in Tokyo and was tentatively named SRSV-9. SRSV-9 was purified by sucrose velocity gradient centrifugation after CsCl density gradient centrifugation. The buoyant density of SRSV-9 appeared to be 1.36 g/ml in CsCl. A Western blot (immunoblot) assay using the biotin-avidin system revealed that SRSV-9 was antigenically related to the Hawaii agent but distinct from the Norwalk agent and contained a single major structural protein with a molecular size of 63.0 +/- 0.6 kilodaltons. The prevalence of SRSV-9 infection in Tokyo was surveyed by the Western blot antibody assay by using a crude virus preparation as the antigen. Seroconversion was observed in 56.5% of the patients involved in the outbreaks from which SRSV was detected by electron microscopy.
Hayashi
Y
Y
Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health, Japan.
Ando
T
T
Utagawa
E
E
Sekine
S
S
Okada
S
S
Yabuuchi
K
K
Miki
T
T
Ohashi
M
M
D000208:Acute Disease; D000914:Antibodies, Viral / Q000032:analysis; D015153:Blotting, Western; D002499:Centrifugation, Density Gradient; D004196:Disease Outbreaks; D005243:Feces / Q000382:microbiology; D005516:Food Microbiology; D005759:Gastroenteritis / Q000453:epidemiology / Q000382:microbiology*; D006801:Humans; D008854:Microscopy, Electron; D014041:Tokyo; D014771:Virion / Q000648:ultrastructure; D014777:Virus Diseases / Q000453:epidemiology / Q000382:microbiology*; D014713:Viruses, Unclassified / Q000276:immunology / Q000302:isolation & purification* / Q000648:ultrastructure
D000914:Antibodies, Viral