title: Effects of the chitin synthesis inhibitor diflubenzuron on development of Ascaris suum and Haemonchus contortus.
abstract: The potential of the chitin synthesis inhibitor diflubenzuron (DFB) to alter the development of the parasitic nematodes (Ascaris suum and Haemonchus contortus was investigated. DFB given orally (10 mg kg-1 per day for 30 days) to sheep inoculated with H. contortus infective larvae did not prevent the establishment of adults or affect fecal egg output. However, there was a significant (greater than 90%) decrease in the number of infective larvae recovered from fecal cultures derived from lambs harboring H. contortus adults that were treated with DFB. DFB did not affect egg hatching. Oral administration (10 mg kg-1 per day for 20 days) of DFB to swine harboring adult A. suum adults had no effect on the adult worm burden or on egg morphology, but eggs removed from worms obtained from DFB-treated swine contained less chitin than eggs removed from untreated control swine. DFB also inhibited chitin synthesis in vitro in the isolated reproductive tract of A. suum adults. These results indicate that DFB at high doses can inhibit the subsequent development of H. contortus larvae in the feces. Since H. contortus larvae lack chitin, DFB may act on these larvae by a mechanism independent of a direct effect on chitin synthesis.
authors.affiliation: Helminthic Diseases Laboratory, U.S.D.A., Beltsville, MD 20705.
authors.forename: R H
authors.initials: RH
authors.lastname: Fetterer
authors.forename: J F
authors.initials: JF
authors.lastname: Urban
authors.forename: R W
authors.initials: RW
authors.lastname: Miller
mesh_terms: D000818:Animals; D001196:Ascariasis / Q000188:drug therapy / Q000662:veterinary*; D001200:Ascaris / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D002686:Chitin / Q000096:biosynthesis*; D004132:Diflubenzuron / Q000494:pharmacology / Q000627:therapeutic use*; D005243:Feces / Q000469:parasitology; D005260:Female; D006188:Haemonchiasis / Q000188:drug therapy / Q000662:veterinary*; D006189:Haemonchus / Q000187:drug effects* / Q000254:growth & development; D007605:Juvenile Hormones / Q000627:therapeutic use*; D008297:Male; D010270:Parasite Egg Count / Q000662:veterinary; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology*; D013552:Swine; D013553:Swine Diseases / Q000469:parasitology*; D014251:Trichostrongyloidea / Q000187:drug effects*; D014252:Trichostrongyloidiasis / Q000662:veterinary*
chemical_list: D007605:Juvenile Hormones; D002686:Chitin; D004132:Diflubenzuron