Effects of beta-D-xylosides on proliferation and matrix formation of adherent fibroblastic cells in mouse bone marrow culture.
Mouse bone marrow cells were seeded on small pieces of cover glasses placed in culture dishes, and after 3 days, the pieces of glass to which only small spindle cells adhered were transferred to another dish containing fresh medium. The adherent small spindle cells proliferated and some of them became large polygonal cells with abundant cytoplasm. When phenyl beta-D-thioxyloside (0.5 mM), an artificial initiator of chondroitin sulfate chain synthesis, was added to the culture medium, the cells showed a marked increase in number as compared to controls, with conversion of about 35% of the cells to large size cells. Immunohistochemical staining with monoclonal antibodies showed that the intercellular matrix of adherent cells consists mainly of a large proteglylcan with chondroitin 6-sulfate side chains. By histochemical analysis, the amount of chondroitin sulfate was shown to be greater in the intercellular matrices of xyloside-treated groups than those of control cultures. The amount of chondroitin sulfate in the growth medium of the adherent cells, as measured by uronic acid analysis, was also significantly increased by treatment with phenyl beta-D-thioxyloside compared with controls.
Nagasaka
T
T
Department of Laboratory Medicine, Nagoya University School of Medicine, Japan.
Sobue
M
M
Niwa
M
M
Yasui
C
C
Nara
Y
Y
Fukatsu
T
T
Nakashima
N
N
Takeuchi
J
J
D000818:Animals; D000911:Antibodies, Monoclonal; D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D002448:Cell Adhesion; D002455:Cell Division / Q000187:drug effects*; D002807:Chondroitin / Q000031:analogs & derivatives*; D002809:Chondroitin Sulfates / Q000096:biosynthesis*; D003094:Collagen / Q000378:metabolism; D046508:Culture Techniques; D005109:Extracellular Matrix / Q000187:drug effects*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005353:Fibronectins / Q000378:metabolism; D006027:Glycosides / Q000494:pharmacology*; D007797:Laminin / Q000378:metabolism; D051379:Mice
D000911:Antibodies, Monoclonal; D005353:Fibronectins; D006027:Glycosides; D007797:Laminin; C010807:xylosides; D002807:Chondroitin; D002809:Chondroitin Sulfates; D003094:Collagen