Studies on pharmacological activation of human serum immunoglobulin G by chemical modification and active subfragments. VIII. Effect of carboxamide-methylated light chain (Fr.I-L) on leukocyte functions.
Human serum immunoglobulin G light chain (Fr.I-L), which was reduced and carboxamide-methylated, showed no effect on formyl-methionyl-leucyl-phenylalanine (FMLP)-induced chemotaxis nor on phagocytosis of yeasts when directly added to guinea pig polymorphonuclear leukocytes (PMNs). However, intravenously administered Fr.I-L inhibited emigration of leukocytes into the peritoneal cavity and promoted phagocytosis of yeasts in a yeast-induced peritonitis model in mice. Moreover, Fr.I-L reduced FMLP-induced chemiluminescence (CL) from PMNs. These facts indicated that the anti-inflammatory action of Fr.I-L was caused by inhibiting emigration of leukocytes into the injured site and scavenging superoxide radicals from the cells.
Mimura
T
T
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Tsujikawa
K
K
Yasuda
N
N
Nakajima
H
H
Lee
B G
BG
Okabe
M
M
Kohama
Y
Y
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002634:Chemotaxis, Leukocyte; D004487:Edema / Q000276:immunology; D006168:Guinea Pigs; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D007147:Immunoglobulin Light Chains / Q000276:immunology*; D007460:Iodoacetamide; D007461:Iodoacetates; D007962:Leukocytes / Q000276:immunology*; D008163:Luminescent Measurements; D008297:Male; D008745:Methylation; D051379:Mice; D008813:Mice, Inbred ICR; D009181:Mycoses / Q000276:immunology; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000276:immunology; D010538:Peritonitis / Q000209:etiology / Q000276:immunology; D010587:Phagocytosis; D013329:Structure-Activity Relationship
D007074:Immunoglobulin G; D007147:Immunoglobulin Light Chains; D007461:Iodoacetates; D009240:N-Formylmethionine Leucyl-Phenylalanine; D007460:Iodoacetamide