Rapid nucleic acid assay for detection of bacteria with tetM-mediated tetracycline resistance.
A novel nucleic acid assay has been developed to screen bacterial populations for the presence of the tetM structural gene. The method involves the specific hybridization of several synthetic oligonucleotides to the gene in a crude bacterial lysate solution. As few as 1.5 x 10(4) CFU can be detected with the assay.
Sanchez-Pescador
R
R
Chiron Corporation, Emeryville, California 94608.
Running
J A
JA
Stempien
M M
MM
Urdea
M S
MS
D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D005798:Genes, Bacterial; D009344:Neisseria gonorrhoeae / Q000032:analysis* / Q000235:genetics; D009693:Nucleic Acid Hybridization; D013753:Tetracycline Resistance
D004269:DNA, Bacterial