Sporulation of Myxococcus xanthus in liquid shake flask cultures.
When suspended in a liquid starvation medium, exponentially growing Myxococcus xanthus sporulated within 3 days. These myxospores were similar to spores developed within fruiting bodies, as determined by electron microscopy and the production of spore-specific protein S. This liquid sporulation system may be useful as a means of preparing large quantities of myxospores and extracellular fluid for biochemical studies, including isolation of chemical signals produced during the sporulation process.
Rosenbluh
A
A
Department of Microbiology, Tel Aviv University, Israel.
Rosenberg
E
E
D001431:Bacteriological Techniques; D003470:Culture Media; D007700:Kinetics; D008854:Microscopy, Electron; D009231:Myxococcales / Q000254:growth & development / Q000502:physiology* / Q000648:ultrastructure; D013171:Spores, Bacterial / Q000502:physiology / Q000648:ultrastructure
D003470:Culture Media