title: Enzyme immunoassay of gastrin releasing peptide (GRP)-like immunoreactivity in milk.
abstract: A sensitive and specific enzyme immunoassay (EIA) for gastrin releasing peptide (GRP)-like immunoreactivity was developed using enzyme-labeled antigen. The synthetic carboxy-terminal fragment of human GRP(12-27) was conjugated with beta-D-galactosidase for EIA. The minimum amount of GRP-like immunoreactivity detectable by this method was 0.24 femtomol/well (6 picomol/liter). The level of GRP-like immunoreactive substance in bovine foremilk was about 150 nanomol/liter, the level of which was more than hundredfold higher than that in normal milk or calf serum.
authors.affiliation: Department of Hospital Pharmacy, Medical College of Oita, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Takeyama
authors.forename: K
authors.initials: K
authors.lastname: Kondo
authors.forename: Y
authors.initials: Y
authors.lastname: Hayashi
authors.forename: H
authors.initials: H
authors.lastname: Yajima
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology; D001839:Bombesin / Q000032:analysis; D002850:Chromatography, Gel; D019886:Gastrin-Releasing Peptide; D007106:Immune Sera / Q000276:immunology; D007124:Immunoenzyme Techniques; D008892:Milk / Q000032:analysis*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis; D010455:Peptides / Q000032:analysis* / Q000276:immunology; D001616:beta-Galactosidase
chemical_list: D000941:Antigens; D007106:Immune Sera; D010446:Peptide Fragments; D010455:Peptides; D019886:Gastrin-Releasing Peptide; C040794:neuromedin C; D001616:beta-Galactosidase; D001839:Bombesin