Human müllerian inhibitory factor messenger ribonucleic acid is hormonally regulated in the fetal testis and in adult granulosa cells.
The regression of the Müllerian ducts (the embryologic precursor of uterus, vagina, and Fallopian tubes) in the male fetus is caused by Müllerian inhibitory factor (MIF), a glycoprotein produced by fetal Sertoli cells. Although this Müllerian duct involution is complete before midgestation, the amount of MIF mRNA did not vary among 25 human fetal testis samples from 13 to 25.8 weeks of gestation. In cultured 20-week human testis cells, cAMP increased MIF mRNA 8.3-fold, but the human gonadotropins FSH and CG had no effect. In cultured adult human granulosa cells, CG and cAMP increased MIF mRNA accumulation to 430% and 890%, respectively, but FSH had no effect. The expression and hormonal regulation of MIF mRNA in midgestation testes and in adult granulosa cells indicate that MIF has physiological roles in the human gonad other than Müllerian duct regression.
Voutilainen
R
R
Department of Pediatrics, University of California, San Francisco 94143.
Miller
W L
WL
D054304:Anti-Mullerian Hormone; D001483:Base Sequence; D003994:Bucladesine / Q000494:pharmacology; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D005260:Female; D005333:Fetus / Q000378:metabolism; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D005786:Gene Expression Regulation; D006023:Glycoproteins; D006107:Granulosa Cells / Q000378:metabolism*; D006131:Growth Inhibitors / Q000235:genetics*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009095:Mullerian Ducts; D015345:Oligonucleotide Probes; D012313:RNA / Q000032:analysis; D012333:RNA, Messenger / Q000378:metabolism*; D012733:Sex Differentiation / Q000502:physiology; D013735:Testicular Hormones / Q000235:genetics*; D013737:Testis / Q000196:embryology* / Q000378:metabolism*
D006063:Chorionic Gonadotropin; D006023:Glycoproteins; D006131:Growth Inhibitors; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D013735:Testicular Hormones; D012313:RNA; D003994:Bucladesine; D054304:Anti-Mullerian Hormone; D005640:Follicle Stimulating Hormone