17 alpha-ethinylestradiol decreases production and release of prostacyclin in cultured human umbilical vein endothelial cells.
Human umbilical vein endothelial cells (HUVEC) were incubated with the estrogen 17 alpha-Ethinylestradiol (30 ng/ml) in order to examine its regulating influence on synthesis and release of prostacyclin (PGI2). ATP (1 mg/ml) was used to stimulate PGI2-production through the purine receptors. We demonstrated that this level of estrogen decreases PGI2-synthesis by 11% and PGI2-release by 32% within 300 sec. Longer incubation times (48 hrs) resulted in the same inhibitory effect. Intracellular ATP content and methyl-3H-thymidine uptake demonstrated that the decrease of prostacyclin-concentration is not caused by reduced viability of the cells but by a direct inhibitory effect on prostacyclin synthesis and release.
David
M
M
2nd Department of Surgery, University of Vienna, Austria.
Griesmacher
A
A
Müller
M M
MM
D000255:Adenosine Triphosphate / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011464:Epoprostenol / Q000096:biosynthesis* / Q000378:metabolism; D004997:Ethinyl Estradiol / Q000494:pharmacology*; D006801:Humans; D013936:Thymidine / Q000378:metabolism; D014471:Umbilical Veins / Q000187:drug effects
D004997:Ethinyl Estradiol; D000255:Adenosine Triphosphate; D011464:Epoprostenol; D013936:Thymidine