Accelerated ion fluxes during differentiation in zoospores of Phytophthora palmivora.
Zoospores of Phytophthora palmivora show increased fluxes of Na+ and Ca2+ 3-5 min after they have been stimulated to differentiate with pectin. Both spontaneous and pectin-induced encystment are reduced below pH 6 and accelerated above pH 7. The ionophores monensin and A23187 induce slow differentiation when added together, but not when added separately. Ethanol (0.5%) also induces slow differentiation. Amiloride and verapamil inhibit pectin-induced differentiation and also reduce the onset of the Na+ and Ca2+ flux. A requirement for Ca2+ for differentiation is confirmed, but a requirement for Na+ could not be demonstrated.
Iser
J R
JR
Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria, Australia.
Griffith
J M
JM
Balson
A
A
Grant
B R
BR
D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000493:pharmacokinetics*; D002454:Cell Differentiation / Q000187:drug effects; D002463:Cell Membrane Permeability / Q000187:drug effects; D008411:Chytridiomycota / Q000502:physiology*; D000431:Ethanol / Q000494:pharmacology; D007474:Ion Exchange; D008985:Monensin / Q000494:pharmacology; D009024:Morphogenesis / Q000187:drug effects; D010838:Phytophthora / Q000502:physiology*; D012964:Sodium / Q000493:pharmacokinetics*; D014700:Verapamil / Q000494:pharmacology
D000001:Calcimycin; D000431:Ethanol; D000584:Amiloride; D008985:Monensin; D012964:Sodium; D014700:Verapamil; D002118:Calcium