Determination of non-enzymatically glycated apolipoprotein A1.
A procedure for the quantitation of non-enzymatically glycated apolipoprotein A1 (GApoA1) was developed and optimized. Glycated total protein was separated from plasma using m-aminophenyl-boronate affinity chromatography. Apolipoprotein A1 present in the glycated and non-glycated fractions of each sample was determined by rate nephelometry, and the percent glycated apo A1 calculated. The measuring range of the assay was 0.5-8.0% GApoA1. The within- and between-run CV's were less than 5.2 and 7.9%, respectively, and recoveries were greater than 92%. Free glucose did not affect the results. In a group of female non-insulin diabetic subjects the mean GApoA1 was 3.8 +/- 1.6% (mean +/- SD). In non-diabetic subjects the mean level of GApoA1 was 2.1 +/- 0.8% (mean +/- SD).
Caines
P S
PS
Department of Chemistry and Biochemistry, University of Windsor, Ontario, Canada.
Thibert
R J
RJ
Draisey
T F
TF
Foreback
C C
CC
Chu
J W
JW
D000328:Adult; D000368:Aged; D016632:Apolipoprotein A-I; D001054:Apolipoproteins A / Q000032:analysis*; D001786:Blood Glucose; D002846:Chromatography, Affinity; D003924:Diabetes Mellitus, Type 2 / Q000097:blood; D006801:Humans; D008875:Middle Aged; D009391:Nephelometry and Turbidimetry; D011134:Polysaccharides / Q000378:metabolism*
D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D001786:Blood Glucose; D011134:Polysaccharides