Endothelin-3 is a novel neuropeptide: isolation and sequence determination of endothelin-1 and endothelin-3 in porcine brain.
The molecular forms of endothelin (ET) related peptides were investigated in porcine brain by using high performance liquid chromatography coupled with three specific radioimmunoassays. ET-1 and its oxidized form were isolated and sequenced as in the case of porcine spinal cord. A very small amount of big ET-1 (1-39) and its C-terminal fragment (big ET-1 (22-39] were also detected. Furthermore, immunoreactive (ir)-ET-3 was isolated and sequenced; its partial primary structure was identical to that of human (rat) ET-3. The concentrations of ir-ET-1 and ir-ET-3 in porcine brain were 140 fmol/g tissue and 5 fmol/g tissue, respectively. These results indicate that besides ET-1, ET-3 is a novel neuropeptide in the central nervous system.
Shinmi
O
O
Department of Biochemistry, University of Tsukuba, Ibaraki, Japan.
Kimura
S
S
Sawamura
T
T
Sugita
Y
Y
Yoshizawa
T
T
Uchiyama
Y
Y
Yanagisawa
M
M
Goto
K
K
Masaki
T
T
Kanazawa
I
I
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D016232:Endothelins; D004730:Endothelium, Vascular / Q000378:metabolism; D010455:Peptides / Q000302:isolation & purification* / Q000378:metabolism; D011863:Radioimmunoassay; D013552:Swine
D016232:Endothelins; D010455:Peptides