title: Reactivity of monoclonal antibody P1 with glomerular basement membrane in thin-membrane nephropathy.
abstract: A monoclonal antibody, P1, possessing antiglomerular basement membrane specificity similar to that of naturally-occurring Goodpasture antibody, was used to study renal biopsy material from patients with thin-membrane nephropathy. Immunofluorescence and immunoperoxidase techniques were employed to detect tissue localisation after sections had been incubated with P1. Staining of glomerular and tubular basement membranes in the 14 patients with thin-membrane nephropathy was similar to that in 10 subjects with various other renal diseases, whereas three patients with Alport's syndrome all gave diminished or absent staining, as has been reported previously. These observations confirm that Goodpasture antigen is present in the basement membranes in thin-membrane nephropathy and that it reacts normally with P1 antibody. They also add to the evidence that the lesions of thin-membrane nephropathy and Alport's syndrome are fundamentally different. The staining method may be used as a differential diagnostic test.
authors.affiliation: Dulwich Hospital, London, UK.
authors.forename: F E
authors.initials: FE
authors.lastname: Dische
authors.forename: I P
authors.initials: IP
authors.lastname: Brooke
authors.forename: S J
authors.initials: SJ
authors.lastname: Cashman
authors.forename: A
authors.initials: A
authors.lastname: Severn
authors.forename: D
authors.initials: D
authors.lastname: Taube
authors.forename: V
authors.initials: V
authors.lastname: Parsons
authors.forename: M
authors.initials: M
authors.lastname: Kershaw
authors.forename: A
authors.initials: A
authors.lastname: Reed
authors.forename: C D
authors.initials: CD
authors.lastname: Pusey
mesh_terms: D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D001485:Basement Membrane / Q000276:immunology; D003937:Diagnosis, Differential; D005260:Female; D015433:Glomerulonephritis, Membranous / Q000175:diagnosis / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D007678:Kidney Glomerulus / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009394:Nephritis, Hereditary / Q000175:diagnosis / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal