Reduced growth hormone releasing factor (GHRF)-like immunoreactivity and GHRF gene expression in the hypothalamus of aged rats.
Growth hormone releasing factor-like immunoreactivity (GHRF-LI) and GHRF mRNA levels were evaluated in the hypothalamus of aged (24 months) and young (3 months) rats by semiquantitative immunocytochemistry and slot blot hybridization technique, respectively. Simultaneous detection of reduced GHRF-LI and GHRF mRNA levels in aged rats as compared to young counterparts demonstrates the existence in aged rats of an impaired function of GHRF-producing neurons.
DeGennaro Colonna
V
V
Department of Pharmacology, University of Milan, Italy.
Zoli
M
M
Cocchi
D
D
Maggi
A
A
Marrama
P
P
Agnati
L F
LF
Müller
E E
EE
D000375:Aging; D000818:Animals; D005786:Gene Expression Regulation; D005796:Genes; D013007:Growth Hormone-Releasing Hormone / Q000032:analysis* / Q000235:genetics; D007031:Hypothalamus / Q000254:growth & development*; D008297:Male; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D014158:Transcription, Genetic
D012333:RNA, Messenger; D013007:Growth Hormone-Releasing Hormone