Inhibitory effects of phenol derivatives on bovine platelet aggregation and their effects on Ca2+ mobilization.
The effects of phenol derivatives on aggregation of bovine platelets induced by ADP, thrombin, platelet activating factor, collagen and A23187 were investigated. The phenol derivatives inhibited all these induced aggregations except that by the calcium ionophore. The derivatives each inhibited the aggregations induced by ADP, thrombin, platelet activating factor and collagen, respectively, within a similar concentration range. A linear relation was found between the inhibitory potencies of the phenol derivatives and their partition coefficients between n-octanol and water (Poct values), suggesting that their interaction with hydrophobic regions of the cell was important for inhibition. Fluorescence analyses with fura-2-loaded platelets showed that in the concentration ranges in which the phenol derivatives inhibited aggregation, they also inhibited agonist-induced increases in Ca2+ both in the presence and absence of extracellular Ca2+. Moreover, a high correlation was found between the inhibitory effects of the derivatives on aggregation and their effects on Ca2+ mobilization. These results suggest that inhibition of platelet aggregation by phenol derivatives is mainly due to inhibition of the increase in cytoplasmic Ca2+ by inhibition of both intracellular Ca2+ mobilization and Ca2+ uptake.
Kitagawa
S
S
Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
Ito
Y
Y
Oda
Y
Y
Kametani
F
F
D020003:1-Octanol; D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D001572:Benzofurans; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000097:blood*; D002417:Cattle; D003094:Collagen / Q000494:pharmacology; D003593:Cytoplasm / Q000378:metabolism; D005456:Fluorescent Dyes; D016257:Fura-2; D000442:Octanols; D010636:Phenols / Q000494:pharmacology*; D010972:Platelet Activating Factor / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors; D012995:Solubility; D013050:Spectrometry, Fluorescence; D013917:Thrombin / Q000494:pharmacology; D014867:Water
D001572:Benzofurans; D005456:Fluorescent Dyes; D000442:Octanols; D010636:Phenols; D010972:Platelet Activating Factor; D010975:Platelet Aggregation Inhibitors; D014867:Water; D000001:Calcimycin; D000244:Adenosine Diphosphate; D003094:Collagen; D013917:Thrombin; D020003:1-Octanol; D002118:Calcium; D016257:Fura-2