title: Studies on pharmacological activation of human serum immunoglobulin G by chemical modification and active subfragments. VIII. Effect of carboxamide-methylated light chain (Fr.I-L) on leukocyte functions.
abstract: Human serum immunoglobulin G light chain (Fr.I-L), which was reduced and carboxamide-methylated, showed no effect on formyl-methionyl-leucyl-phenylalanine (FMLP)-induced chemotaxis nor on phagocytosis of yeasts when directly added to guinea pig polymorphonuclear leukocytes (PMNs). However, intravenously administered Fr.I-L inhibited emigration of leukocytes into the peritoneal cavity and promoted phagocytosis of yeasts in a yeast-induced peritonitis model in mice. Moreover, Fr.I-L reduced FMLP-induced chemiluminescence (CL) from PMNs. These facts indicated that the anti-inflammatory action of Fr.I-L was caused by inhibiting emigration of leukocytes into the injured site and scavenging superoxide radicals from the cells.
authors.affiliation: Faculty of Pharmaceutical Sciences, Osaka University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Mimura
authors.forename: K
authors.initials: K
authors.lastname: Tsujikawa
authors.forename: N
authors.initials: N
authors.lastname: Yasuda
authors.forename: H
authors.initials: H
authors.lastname: Nakajima
authors.forename: B G
authors.initials: BG
authors.lastname: Lee
authors.forename: M
authors.initials: M
authors.lastname: Okabe
authors.forename: Y
authors.initials: Y
authors.lastname: Kohama
mesh_terms: D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002634:Chemotaxis, Leukocyte; D004487:Edema / Q000276:immunology; D006168:Guinea Pigs; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D007147:Immunoglobulin Light Chains / Q000276:immunology*; D007460:Iodoacetamide; D007461:Iodoacetates; D007962:Leukocytes / Q000276:immunology*; D008163:Luminescent Measurements; D008297:Male; D008745:Methylation; D051379:Mice; D008813:Mice, Inbred ICR; D009181:Mycoses / Q000276:immunology; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000276:immunology; D010538:Peritonitis / Q000209:etiology / Q000276:immunology; D010587:Phagocytosis; D013329:Structure-Activity Relationship
chemical_list: D007074:Immunoglobulin G; D007147:Immunoglobulin Light Chains; D007461:Iodoacetates; D009240:N-Formylmethionine Leucyl-Phenylalanine; D007460:Iodoacetamide