A comparison of six monoclonal antibodies for detection of cytokeratins in normal and neoplastic canine tissues.
Twenty normal canine tissue specimens, both fetal and adult; 19 epithelial neoplasms; and 18 nonepithelial neoplasms were examined using 6 commercially available monoclonal antibodies differing in their recognition of various molecular weight cytokeratins in human tissues. Fresh tissue samples were fixed in 100% ethanol and paraffin embedded prior to sectioning. The intermediate filament proteins were identified by an avidin-biotin-immunoperoxidase method. Primary antisera used included AE1/AE3, CAM-5.2, 35BH11, 34BE12, PKK1, MAK-6 cytokeratins, and vimentin. Monoclonal antibodies detected cytokeratins in a wide variety of canine epithelial tissues and neoplasms. Normal mesenchymal tissues and neoplasms, and stromal elements of epithelial tissues, showed no reactivity with anti-cytokeratins, but reacted positively with vimentin. Although PKK1, CAM-5.2, and MAK-6 were the most consistently reactive anti-cytokeratins, the full panel of monoclonals was required to detect cytokeratins in all of the epithelia evaluated.
Cardona
A
A
Veterinary Medical Teaching Hospital, University of California, Davis 95616.
Madewell
B R
BR
Naydan
D K
DK
Lund
J K
JK
D000818:Animals; D000911:Antibodies, Monoclonal; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D004848:Epithelium / Q000737:chemistry; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis* / Q000276:immunology; D009369:Neoplasms / Q000737:chemistry / Q000175:diagnosis / Q000662:veterinary*
D000911:Antibodies, Monoclonal; D007633:Keratins