Changes in nucleosome repeat lengths precede replication in the early replicating metallothionein II gene region of cells synchronized in early S phase.
Previous investigations showed that inhibition of DNA synthesis by hydroxyurea, aphidicolin, or 5-fluorodeoxyuridine produced large changes in the composition and nucleosome repeat lengths of bulk chromatin. Here we report results of investigations to determine whether the changes in nucleosome repeat lengths might be localized in the initiated replicons, as postulated [D'Anna, J. A., & Prentice, D. A. (1983) Biochemistry 22, 5631-5640]. In most experiments, Chinese hamster (line CHO) cells were synchronized in G1, or they were synchronized in early S phase by allowing G1 cells to enter S phase in medium containing 1 mM hydroxyurea or 5 micrograms mL-1 aphidicolin, a procedure believed to produce an accumulation of initiated replicons that arise from normally early replicating DNA. Measurements of nucleosome repeat lengths of bulk chromatin, the early replicating unexpressed metallothionein II (MTII) gene region, and a later replicating repeated sequence indicate that the changes in repeat lengths occur preferentially in the early replicating MTII gene region as G1 cells enter and become synchronized in early S phase. During that time, the MTII gene region is not replicated nor is there any evidence for induction of MTII messenger RNA. Thus, the results are consistent with the hypothesis that changes in chromatin structure occur preferentially in the early replicating (presumably initiated) replicons at initiation or that changes in chromatin structure can precede replication during inhibition of DNA synthesis. The shortened repeat lengths that precede MTII replication are, potentially, reversible, because they become elongated when the synchronized early S-phase cells are released to resume cell cycle progression.
D'Anna
J A
JA
Life Sciences Division, Los Alamos National Laboratory, New Mexico 87545.
Tobey
R A
RA
D000818:Animals; D016590:Aphidicolin; D015139:Blotting, Southern; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002453:Cell Cycle; D002460:Cell Line; D002843:Chromatin / Q000378:metabolism / Q000648:ultrastructure; D004247:DNA / Q000235:genetics; D004261:DNA Replication; D004224:Diterpenes / Q000494:pharmacology; D005796:Genes; D006918:Hydroxyurea / Q000494:pharmacology; D007700:Kinetics; D008668:Metallothionein / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D009707:Nucleosomes / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D012091:Repetitive Sequences, Nucleic Acid
D002843:Chromatin; D004224:Diterpenes; D009707:Nucleosomes; D016590:Aphidicolin; D004247:DNA; D008668:Metallothionein; D001973:Bromodeoxyuridine; D006918:Hydroxyurea