title: Determination of sulfadimethoxine, sulfamethoxazole, trimethoprim and their main metabolites in porcine plasma by column switching HPLC.
abstract: A HPLC method for the determination of sulfadimethoxine, sulfamethoxazole, trimethoprim and their main metabolites in porcine plasma is reported. The metabolites under investigation were the N4-acetyl sulfonamides and 3'- and 4'-demethyl trimethoprim. In order to obtain a sensitivity of 25-50 ng ml-1, the application of column switching HPLC was investigated. An on-line preconcentration of the drugs and metabolites was preceded by an off-line sample pre-treatment. Parent compounds and metabolites were separated by reversed-phase HPLC followed by UV-detection. The mean recoveries for 4'-demethyl trimethoprim were greater than 80% while the mean recoveries for the other compounds were greater than 90%. Application of the method for analysis of plasma samples obtained from pharmacokinetic studies is described.
authors.affiliation: State Institute for Quality Control of Agricultural Products, Wageningen, The Netherlands.
authors.forename: M J
authors.initials: MJ
authors.lastname: Mengelers
authors.forename: M B
authors.initials: MB
authors.lastname: Oorsprong
authors.forename: H A
authors.initials: HA
authors.lastname: Kuiper
authors.forename: M M
authors.initials: MM
authors.lastname: Aerts
authors.forename: E R
authors.initials: ER
authors.lastname: van Gogh
authors.forename: A S
authors.initials: AS
authors.lastname: van Miert
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D013056:Spectrophotometry, Ultraviolet; D013412:Sulfadimethoxine / Q000097:blood* / Q000493:pharmacokinetics; D013420:Sulfamethoxazole / Q000097:blood* / Q000493:pharmacokinetics; D013552:Swine; D014295:Trimethoprim / Q000097:blood* / Q000493:pharmacokinetics
chemical_list: D007202:Indicators and Reagents; D013412:Sulfadimethoxine; D014295:Trimethoprim; D013420:Sulfamethoxazole