Preliminary crystallographic data and primary sequence for anti-peptide Fab' B13I2 and its complex with the C-helix peptide from myohemerythrin.
Crystals of the Fab' fragment from the monoclonal anti-peptide antibody B1312 and of the Fab'-peptide antigen complex have been characterized. The monoclonal antibodies were raised against a synthetic homologue of the C-helix of myohemerythrin (residues 69-87 in myohemerythrin). The Fab'-peptide complex crystallizes in space group P6322 with unit cell dimensions a = b = 142.5 A, c = 101.5 A, alpha = beta = 90 degrees, gamma = 120 degrees, and Z = 1. The native Fab' crystallizes in space group P212121 with unit cell dimensions a = 98.0 A, b = 151.7 A, c = 80.8 A, alpha = beta = gamma = 90 degrees, and Z = 2. Both crystal forms diffract to beyond 2.6 A resolution. We also report the cDNA and predicted amino acid sequences for the variable regions of both the light and heavy chains of this anti-peptide antibody.
Stura
E A
EA
Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, California 92037.
Stanfield
R L
RL
Fieser
T M
TM
Balderas
R S
RS
Smith
L R
LR
Lerner
R A
RA
Wilson
I A
IA
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000235:genetics; D000936:Antigen-Antibody Complex / Q000302:isolation & purification; D001483:Base Sequence; D003460:Crystallization; D006422:Hemerythrin / Q000031:analogs & derivatives / Q000276:immunology; D007140:Immunoglobulin Fab Fragments / Q000235:genetics / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000235:genetics / Q000302:isolation & purification; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007147:Immunoglobulin Light Chains / Q000235:genetics; D008667:Metalloproteins / Q000031:analogs & derivatives / Q000276:immunology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification; D011487:Protein Conformation; D014961:X-Ray Diffraction
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D006422:Hemerythrin; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D008667:Metalloproteins; D010446:Peptide Fragments; C008571:myohemerythrin