title: Fasciclin III: a novel homophilic adhesion molecule in Drosophila.
abstract: Drosophila fasciclin III is an integral membrane glycoprotein that is expressed on a subset of neurons and fasciculating axons in the developing CNS, as well as in several other tissues during development. Here we report on the isolation of a full-length cDNA encoding an 80 kd form of fasciclin III. We have used this cDNA, under heat shock control, to transfect the relatively nonadhesive Drosophila S2 cell line. Examination of these transfected cells indicates that fasciclin III is capable of mediating adhesion in a homophilic, Ca2+-independent manner. Sequence analysis reveals that fasciclin III encodes a transmembrane protein with no significant homology to any known protein, including the previously characterized families of vertebrate cell adhesion molecules. The distribution of this adhesion molecule on subsets of fasciculating axons and growth cones during Drosophila development suggests that fasciclin III plays a role in growth cone guidance.
authors.affiliation: Howard Hughes Medical Institute, University of California, Berkeley 94720.
authors.forename: P M
authors.initials: PM
authors.lastname: Snow
authors.forename: A J
authors.initials: AJ
authors.lastname: Bieber
authors.forename: C S
authors.initials: CS
authors.lastname: Goodman
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002448:Cell Adhesion; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011487:Protein Conformation; D014162:Transfection
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D029721:Drosophila Proteins; C061565:Fas3 protein, Drosophila; D004247:DNA