Mechanism of interleukin-2 signaling: mediation of different outcomes by a single receptor and transduction pathway.
The T cell lymphokine, interleukin-2 (IL-2), plays a pivotal role in an immune response by stimulating antigen-activated B lymphocytes to progress through the cell cycle and to differentiate into antibody-secreting cells. An IL-2 inducible B lymphoma line, in which the growth and differentiation responses are uncoupled, provides a model system for dissecting the signaling mechanisms operating in each response. This system was used to show that both signals are initiated by IL-2 binding to a single, unifunctional receptor complex. Moreover, both signals are transduced by a pathway that does not involve any known second messenger system and that can be blocked by a second T cell lymphokine, interleukin 4. These findings suggest that the pleiotrophic effects of IL-2 are determined by different translations of the signal in the nucleus.
Tigges
M A
MA
Chiron Corporation, Emeryville, CA 94608.
Casey
L S
LS
Koshland
M E
ME
D000818:Animals; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000502:physiology*; D002118:Calcium / Q000502:physiology; D005786:Gene Expression Regulation; D007144:Immunoglobulin J-Chains / Q000235:genetics; D007376:Interleukin-2 / Q000502:physiology*; D015847:Interleukin-4; D007378:Interleukins / Q000494:pharmacology; D008213:Lymphocyte Activation; D051379:Mice; D011493:Protein Kinase C / Q000502:physiology; D015375:Receptors, Interleukin-2 / Q000502:physiology*; D014407:Tumor Cells, Cultured
D007144:Immunoglobulin J-Chains; D007376:Interleukin-2; D007378:Interleukins; D015375:Receptors, Interleukin-2; D015847:Interleukin-4; D011493:Protein Kinase C; D002118:Calcium