Synthesis of an inhibitor of human immunodeficiency virus infection.
Anti-HIV effects of lentinan sulfate were investigated by using an HTLV-I-carrying cell line, MT-4, in vitro. Lentinan, a fungal branched (1----3)-beta-D-glucan, was sulfated to various degrees by means of two kinds of procedures using piperidine N-sulfonic acid in dimethyl sulfoxide or chlorosulfonic acid in pyridine. Lentinan sulfate with a sulfur content of more than 13.9% effectively prevented HIV-induced cytopathic effects (CPE) at concentrations of more than 3.3 micrograms/ml. However, low-substituted lentinan sulfate did not prevent HIV-induced CPE at any concentration tested. When the countercation was 50% Na+ and 50% pyridinium ion, the inhibitory capacity was low. Anticoagulant activity of the lentinan sulfate was also assessed.
Hatanaka
K
K
Institute of Industrial Science, University of Tokyo.
Yoshida
T
T
Uryu
T
T
Yoshida
O
O
Nakashima
H
H
Yamamoto
N
N
Mimura
T
T
Kaneko
Y
Y
D000925:Anticoagulants; D000998:Antiviral Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D005455:Fluorescent Antibody Technique; D006678:HIV / Q000187:drug effects*; D015488:HIV Antigens / Q000096:biosynthesis; D015368:Human T-lymphotropic virus 1; D006801:Humans; D007912:Lentinan / Q000031:analogs & derivatives / Q000138:chemical synthesis* / Q000494:pharmacology / Q000627:therapeutic use; D011134:Polysaccharides / Q000138:chemical synthesis*; D013455:Sulfur / Q000378:metabolism
D000925:Anticoagulants; D000998:Antiviral Agents; D015488:HIV Antigens; D011134:Polysaccharides; C059822:lentinan sulfate; D007912:Lentinan; D013455:Sulfur