Detection of extended broad-spectrum beta-lactamases in Enterobacteriaceae in four French hospitals.
In 210 strains of Enterobacteriaceae which were isolated in four hospitals and which showed reduced susceptibility to cefotaxime, high synergy was demonstrated between amoxicillin (20 micrograms) + clavulanate (10 micrograms) and cefotaxime (30 micrograms) using a simple double-disk test. Isoelectric focusing on gel and specific iodometric detection using ceftriaxone identified four extended broad-spectrum beta-lactamases (isoelectric points 7.6, 6.3, 7.0 and 5.9) produced by the strains.
Legrand
P
P
Service de Bactériologie, Hôpital Henri Mondor, Créteil, France.
Fournier
G
G
Buré
A
A
Jarlier
V
V
Nicolas
M H
MH
Decré
D
D
Duval
J
J
Philippon
A
A
D000658:Amoxicillin / Q000494:pharmacology*; D019980:Amoxicillin-Potassium Clavulanate Combination; D002439:Cefotaxime / Q000494:pharmacology*; D002954:Citrobacter / Q000187:drug effects / Q000201:enzymology / Q000302:isolation & purification; D002969:Clavulanic Acids / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D004357:Drug Synergism; D004359:Drug Therapy, Combination / Q000494:pharmacology; D004754:Enterobacter / Q000187:drug effects / Q000201:enzymology / Q000302:isolation & purification; D004755:Enterobacteriaceae / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000302:isolation & purification; D005602:France; D006801:Humans; D008826:Microbial Sensitivity Tests; D010403:Penicillin Resistance; D012706:Serratia marcescens / Q000187:drug effects / Q000201:enzymology / Q000302:isolation & purification; D001618:beta-Lactamases / Q000378:metabolism*
D002969:Clavulanic Acids; D019980:Amoxicillin-Potassium Clavulanate Combination; D000658:Amoxicillin; D001618:beta-Lactamases; D002439:Cefotaxime