Detection of individual interleukin 4- and gamma interferon-producing murine spleen cells after activation with T-cell mitogens.
Murine spleen cells were activated with concanavalin A (Con A), pokeweed mitogen (PWM), or phorbol myristate acetate (PMA) and the calcium ionophore A23187. Cells producing gamma interferon (IFN-gamma) or interleukin 4 (IL-4) could be detected by lymphokine-specific monoclonal antibodies and indirect immunofluorescence. The frequency and kinetics of the lymphokine-producing cells were examined and were approximately the same after stimulation with Con A or PMA and A23187. Thirty hours after activation, 3-9% of the cells produced IFN-gamma. There were few IL-4-producing cells, and the maximal frequency was 1 out of 400 spleen cells 48 h after activation. When the cells were activated with PWM, the frequency of IFN-gamma-producing cells was still high 72 h after culture. The majority of the IFN-gamma-producing cells were CD8+ and expressed receptors for IL-2.
Sander
B
B
Department of Immunology, Arrhenius Laboratories for Natural Science, University of Stockholm, Sweden.
Cardell
S
S
Heremans
H
H
Andersson
U
U
Möller
G
G
D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology; D007371:Interferon-gamma / Q000096:biosynthesis*; D015847:Interleukin-4; D007378:Interleukins / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D013154:Spleen / Q000276:immunology*; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D007378:Interleukins; D003208:Concanavalin A; D015847:Interleukin-4; D007371:Interferon-gamma; D013755:Tetradecanoylphorbol Acetate