title: Localization of the replication origin of plasmid pE194.
abstract: The pE194 replication origin was localized to a 265-base-pair interval by analyzing the ability of purified pE194 restriction fragments to direct replication of heterologous plasmids. Replication was dependent upon RepF protein supplied in trans. The origin region contained a GC-rich dyad symmetry which may serve as the RepF target.
authors.affiliation: Department of Microbiology, Public Health Research Institute, New York, New York 10016.
authors.forename: L A
authors.initials: LA
authors.lastname: Dempsey
authors.forename: D A
authors.initials: DA
authors.lastname: Dubnau
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D011815:R Factors; D012045:Regulatory Sequences, Nucleic Acid; D012093:Replicon; D015183:Restriction Mapping; D013210:Staphylococcus / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D004269:DNA, Bacterial