Possible involvement of microfilaments in protein kinase C translocation.
We investigated the role of microfilaments in stimulus-induced translocation of protein kinase C (PKC) in polymorphonuclear leukocytes (PMNs) from C57BL/6 mice. Cytochalasin B and dihydrocytochalasin B almost completely inhibited PKC translocation induced by either TPA or Ca2+ ionophore after pretreatment of cells for 30 min. In addition, ML-9, a potent inhibitor of Ca2+/calmodulin-dependent myosin light chain kinase which regulate microfilament contraction, and a calmodulin antagonist W-7, also inhibited PKC translocation. These findings suggest the possibility that microfilaments are involved in the translocation of PKC.
Ito
M
M
Department of Bacteriology, Fukushima Medical College, Japan.
Tanabe
F
F
Sato
A
A
Ishida
E
E
Takami
Y
Y
Shigeta
S
S
D008841:Actin Cytoskeleton / Q000201:enzymology*; D000818:Animals; D001381:Azepines / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D000001:Calcimycin / Q000494:pharmacology; D002147:Calmodulin / Q000037:antagonists & inhibitors; D003571:Cytochalasin B / Q000031:analogs & derivatives / Q000494:pharmacology; D003599:Cytoskeleton / Q000201:enzymology*; D003600:Cytosol / Q000201:enzymology; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009219:Myosin-Light-Chain Kinase / Q000037:antagonists & inhibitors; D009504:Neutrophils / Q000201:enzymology / Q000648:ultrastructure*; D011493:Protein Kinase C / Q000378:metabolism*; D013449:Sulfonamides / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D001381:Azepines; D002147:Calmodulin; D013449:Sulfonamides; C056218:ML 9; D000001:Calcimycin; C024794:dihydrocytochalasin B; D003571:Cytochalasin B; C017967:W 7; D011493:Protein Kinase C; D009219:Myosin-Light-Chain Kinase; D013755:Tetradecanoylphorbol Acetate