title: Modulation of lactate dehydrogenase activity by enzyme-protein interaction.
abstract: Some lactate dehydrogenase modulator proteins have been isolated from the lactate dehydrogenase-free crude mitochondrial fraction of rabbit muscle, beef liver and chicken liver. It was shown that beef and chicken liver mitochondrial extracts exhibited activatory capacity in contrast to the inhibitory capacity of rabbit muscle mitochondrial extracts. All modulators can be precipitated by 80% ammonium sulphate saturation and show high anodic electrophoretic mobility and heat stability. Modulators have higher affinity for alkaline pI lactate dehydrogenase isoenzymes, independent of whether the M and H subunits are predominant. The inhibitor and the activator molecules compete for lactate dehydrogenase since their modulatory capacity was nullified when similar relative amounts were used. This study shows the existence of analogous proteins with an acidic pI in the different mitochondrial fractions which modify lactate dehydrogenase activity.
authors.affiliation: Department of Biochemistry and Physiology, Faculty of Chemistry, University of Barcelona, Spain.
authors.forename: M L
authors.initials: ML
authors.lastname: Sagristá
authors.forename: J
authors.initials: J
authors.lastname: Pruñonosa
authors.forename: C
authors.initials: C
authors.lastname: Lluis
mesh_terms: D000818:Animals; D002417:Cattle; D002645:Chickens; D003600:Cytosol / Q000201:enzymology; D004589:Electrophoresis, Disc; D007525:Isoelectric Focusing; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000037:antagonists & inhibitors* / Q000302:isolation & purification; D008099:Liver / Q000201:enzymology*; D008930:Mitochondria, Liver / Q000201:enzymology*; D008931:Mitochondria, Muscle / Q000201:enzymology*; D011506:Proteins / Q000302:isolation & purification / Q000502:physiology*; D011817:Rabbits
chemical_list: D007527:Isoenzymes; D011506:Proteins; D007770:L-Lactate Dehydrogenase