Quantitative immunohistochemistry: standardization and possible application in research and surgical pathology.
We describe three different methods of calibration of quantitative immunohistochemistry, two of them based on self-made dye-agarose or antigen-agarose substrates. Quantification of immunostained objects (immunosignals) can be done by (1) microscope photometry (plug method) or scanning-photometry; (2) and densitometry of a television transformed microscope image. Both approaches are subjected to the different methods of calibration and three examples of application.
Fritz
P
P
Pathologisches Institut des Robert-Bosch-Krankenhauses, Stuttgart, FRG.
Hönes
J
J
Lutz
D
D
Multhaupt
H
H
Mischlinski
A
A
Dörrer
A
A
Schwarzmann
P
P
Tuczek
H V
HV
Müller
W
W
D001119:Arginase / Q000032:analysis; D001172:Arthritis, Rheumatoid / Q000473:pathology; D001940:Breast / Q000473:pathology; D001943:Breast Neoplasms / Q000473:pathology; D002461:Cell Line, Transformed; D003720:Densitometry / Q000379:methods; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007124:Immunoenzyme Techniques; D007136:Immunoglobulins / Q000032:analysis; D007150:Immunohistochemistry / Q000379:methods*; D010783:Photometry / Q000379:methods; D011960:Receptors, Estrogen / Q000032:analysis; D013583:Synovial Membrane / Q000473:pathology
D007136:Immunoglobulins; D011960:Receptors, Estrogen; D001119:Arginase