A novel VHDJH to JH joining that induces H chain production in an Ig-null immature B cell line.
A novel Ig H chain gene rearrangement, a VHDJH to JH joining, was observed in an Ig-null immature B cell line. The preexisting, nonproductive VHDJH complex was replaced by the productive VHJH complex which was generated by the novel joining between the rearranged VH and a germ line JH gene. This VHDJH to JH joining is thought to be a site-specific recombinational event mediated by a putative recombination signal sequence, CACAGCC-12-base-GCAAGAAAG, embedded in the rearranged VH gene including the N region. This sequence might be a novel recombination signal sequence, which had not yet been reported, for so-called recombinase.
Komori
T
T
Third Department of Internal Medicine, Osaka University Medical School, Japan.
Sugiyama
H
H
Kishimoto
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism* / Q000502:physiology; D001483:Base Sequence; D015139:Blotting, Southern; D002454:Cell Differentiation; D002461:Cell Line, Transformed; D002469:Cell Separation; D015322:Gene Rearrangement, B-Lymphocyte; D007143:Immunoglobulin Heavy Chains / Q000096:biosynthesis* / Q000235:genetics; D007133:Immunoglobulin Joining Region / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D008215:Lymphocytes, Null / Q000378:metabolism* / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data
D007143:Immunoglobulin Heavy Chains; D007133:Immunoglobulin Joining Region; D007135:Immunoglobulin Variable Region