title: Autocide AMI rescues development in dsg mutants of Myxococcus xanthus.
abstract: Low concentrations of autocide AMI rescued aggregation and sporulation in the dsg mutant class of Myxococcus xanthus but were incapable of rescuing asg, bsg, or csg mutants. AMI-induced spores of dsg mutants were resistant to heat and sonication and germinated when plated on nutrient-rich agar. AMI accelerated aggregation and sporulation and increased the final spore number in submerged cultures of a wild-type strain of M. xanthus. Development of M. xanthus was accompanied by release of a fluorescent material (emission maximum, 438 nm) into the supernatant fluid. The release of this material began early and continued throughout development. All Spo- mutant strains tested released significantly reduced levels of this material. These levels were increased in the presence of AMI in all Spo- mutant classes, most dramatically in the dsg mutants.
authors.affiliation: Department of Microbiology, Tel Aviv University, Ramat Aviv, Israel.
authors.forename: A
authors.initials: A
authors.lastname: Rosenbluh
authors.forename: E
authors.initials: E
authors.lastname: Rosenberg
mesh_terms: D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D005227:Fatty Acids / Q000502:physiology*; D005231:Fatty Acids, Unsaturated / Q000502:physiology*; D007700:Kinetics; D009154:Mutation; D009231:Myxococcales / Q000235:genetics / Q000254:growth & development*; D013171:Spores, Bacterial / Q000502:physiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; C058779:autocide AMI