Thermal denaturation of alpha-crystallin.
Low molecular weight alpha-crystallin, isolated from cow lenses, exhibit irreversible thermal denaturation in solution at 75 degrees C. The heat of denaturation was obtained from a differential scanning calorimetric endothermic peak. A second run on the denatured alpha-crystallin solution showed that the denaturation is irreversible. Viscosimetric study and ultraviolet absorption spectroscopy also indicated irreversible denaturation.
Castoro
J A
JA
Chemistry Department, Adelphi University, Garden City, NY 11530.
Bettelheim
F A
FA
D000818:Animals; D002152:Calorimetry, Differential Scanning; D002417:Cattle; D003459:Crystallins / Q000737:chemistry* / Q000302:isolation & purification; D006358:Hot Temperature; D008027:Light; D011489:Protein Denaturation; D012542:Scattering, Radiation; D013056:Spectrophotometry, Ultraviolet; D014783:Viscosity
D003459:Crystallins