Dot microfiltration sandwich EIA for the measurement of IGM concentrations in cerebrospinal fluid.
A sensitive and simple sandwich enzyme immunoassay (EIA) using a dot microfiltration apparatus has been developed for the measurement of immunoglobulin M (IgM) concentrations in cerebrospinal fluid (CSF). The lower detection limit was 0.5 micrograms/dl. We applied this method to the testing of 200 CSF samples with normal concentrations of albumin, IgG and total protein, and normal cell counting. The non-parametric 2.5 and 97.5 percentile limits of IgM and the IgM index were 6.1-64.3 micrograms/dl and 0.012-0.063, respectively. We also applied this method to the testing of samples from 11 cases of multiple sclerosis (MS) and 15 cases of Guillain-Barré syndrome (GBS). High values for IgM and the IgM index were observed in 18.2% and 9.1% of MS, respectively and in 66.6% and 20.0% of GBS, respectively.
Araga
S
S
Division of Neurology, Tottori University School of Medicine, Yonago, Japan.
Kishimoto
M
M
Irie
H
H
Takahashi
K
K
D005069:Evaluation Studies as Topic; D006801:Humans; D007124:Immunoenzyme Techniques; D007075:Immunoglobulin M / Q000134:cerebrospinal fluid*; D009103:Multiple Sclerosis / Q000276:immunology; D009391:Nephelometry and Turbidimetry; D011129:Polyradiculoneuropathy / Q000276:immunology; D012016:Reference Values
D007075:Immunoglobulin M