State transitions in a phycobilisome-less mutant of the cyanobacterium Synechococcus sp. PCC 7002.
State transitions were investigated in the cyanobacterium Synechococcus sp. PCC 7002 in both wild-type cells and mutant cells lacking phycobilisomes. Preillumination in the presence of DCMU induced State 1 and dark-adaptation induced State 2 in both wild-type and mutant cells as determined by 77 K fluorescence emission spectroscopy. Light-induced transitions were observed in the wild-type after preferential excitation of phycocyanin (State 2) or preferential excitation of Chl a (State 1). Light-induced transitions were also observed in the phycobilisome-less mutant after preferential excitation of short-wavelength Chl a (State 2) or carotenoids and long-wavelength Chl a (State 1). We conclude that the mechanism of the light-state transition in cyanobacteria does not require the presence of the phycobilisome. Our results contradict proposed models for the state transition, which require phosphorylation of, and an active role for, the phycobilisome.
Bruce
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Department of Biological Sciences, Brock University, St. Catharines, Canada.
Brimble
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Bryant
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D002734:Chlorophyll / Q000032:analysis / Q000378:metabolism*; D000077194:Chlorophyll A; D000458:Cyanobacteria / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D003624:Darkness; D004237:Diuron / Q000494:pharmacology; D008027:Light; D045342:Light-Harvesting Protein Complexes; D009154:Mutation; D010084:Oxidation-Reduction; D010101:Oxygen Consumption / Q000187:drug effects; D010766:Phosphorylation; D045322:Photosynthetic Reaction Center Complex Proteins; D045524:Phycobilisomes; D010798:Phycocyanin / Q000032:analysis; D010940:Plant Proteins / Q000032:analysis / Q000378:metabolism* / Q000502:physiology*; D011190:Potassium Cyanide / Q000494:pharmacology; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry
D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D045524:Phycobilisomes; D010940:Plant Proteins; C002497:allophycocyanin; D010798:Phycocyanin; D002734:Chlorophyll; D004237:Diuron; D011190:Potassium Cyanide; D000077194:Chlorophyll A