title: Transformation of Penicillium chrysogenum to sulfonamide resistance.
abstract: Penicillium chrysogenum has been transformed to sulfonamide resistance by vectors containing the dihydropteroate synthetase gene from plasmid R388 controlled by the promoter and terminator sequences of the P. chrysogenum trpC gene. Transformation frequencies of four to ten transformants per microgram of vector DNA were obtained.
authors.affiliation: Departamento de Genética Molecular, Antibióticos, Madrid, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Carramolino
authors.forename: M
authors.initials: M
authors.lastname: Lozano
authors.forename: A
authors.initials: A
authors.lastname: Pérez-Aranda
authors.forename: V
authors.initials: V
authors.lastname: Rubio
authors.forename: F
authors.initials: F
authors.lastname: Sánchez
mesh_terms: D015139:Blotting, Southern; D003001:Cloning, Molecular; D004094:Dihydropteroate Synthase / Q000235:genetics*; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D005819:Genetic Markers; D005822:Genetic Vectors; D010407:Penicillium / Q000235:genetics*; D010408:Penicillium chrysogenum / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D011401:Promoter Regions, Genetic; D011815:R Factors; D013449:Sulfonamides / Q000302:isolation & purification; D014166:Transferases / Q000235:genetics*; D014170:Transformation, Genetic
chemical_list: D005819:Genetic Markers; D013449:Sulfonamides; D014166:Transferases; D004094:Dihydropteroate Synthase