T-cell colony formation in healthy homosexual men without anti-human immunodeficiency virus antibody.
In order to study the abnormal differentiation and proliferation of precursor T cells in homosexual men, a study was made of T-cell colony formation in 15 healthy homosexual men without anti-human immunodeficiency virus (HIV) antibody. The colony forming method used was the one-step monolayer technique using phytohemagglutinin as an inducer. Our results revealed that colony formation was extremely reduced and 3 cases showed no colony growth. However, with the addition of recombinant interleukin 2 to the culture system, the number of colonies increased up to the same level as in heterosexual controls. In addition, the analysis of CD3+, 4+, and 8+ cells of colony component cells revealed a decrease in CD3+ and 4+ cells. However, with the addition of recombinant interleukin 2, the numbers of CD3+ cells distinctly increased. The above results suggest that a disorder in the differentiation and proliferation of precursor T cells in homosexual men might be one of the causes of immunodeficiency.
Wakabayashi
Y
Y
Department of Internal Medicine, Juntendo University School of Medicine, Tokyo, Japan.
Sugimoto
M
M
Ishiyama
T
T
Hashimoto
M
M
Horie
S
S
Hirose
S
S
D000328:Adult; D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D006716:Homosexuality; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D013601:T-Lymphocytes / Q000166:cytology* / Q000187:drug effects
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D007376:Interleukin-2