A method for obtaining large numbers of trophozoites of avirulent strains of Toxoplasma gondii using an antibody to interferon-gamma.
Injection of cysts of avirulent strains of T. gondii intraperitoneally into mice that were pretreated with monoclonal antibody against interferon-gamma (IFN-gamma) provided more than 10(7) trophozoites of those strains. This method is suitable for obtaining large numbers of trophozoites of T. gondii strains that are avirulent for mice.
Suzuki
Y
Y
Department of Immunology and Infectious Diseases, Palo Alto Medical Foundation, California 94301.
Remington
J S
JS
D000818:Animals; D000911:Antibodies, Monoclonal; D005260:Female; D007371:Interferon-gamma / Q000276:immunology*; D008722:Methods; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins; D014122:Toxoplasma / Q000302:isolation & purification* / Q000472:pathogenicity; D014774:Virulence
D000911:Antibodies, Monoclonal; D011994:Recombinant Proteins; D007371:Interferon-gamma