Exercise-mediated peripheral tissue and whole-body amino acid metabolism during intravenous feeding in normal man.
1. The effect of a daily submaximal exercise regimen on whole-body and peripheral tissue amino acid metabolism during weight-stable intravenous feeding (IVF) was evaluated in 11 normal volunteers. Five of the subjects performed 1 h of daily bicycle exercise at 75 W during IVF, while the remaining six subjects received IVF without daily exercise. Body nitrogen balance, leg and forearm plasma amino acid flux and whole-body kinetics were measured before and on day 10 of IVF using a [1-13C]leucine and [15N]glycine tracer. 2. At the end of the IVF period, exercised subjects demonstrated leg uptake of total amino acids (237 +/- 103 nmol min-1 100 ml-1 of tissue, mean +/- SEM) which was significantly (P less than 0.05) different than in non-exercised subjects (-1101 +/- 253 nmol min-1 100 ml-1 of tissue). 3. In the non-exercised forearm, a significant (P less than 0.05) decrease in total amino acid flux was observed in exercised subjects (-162 +/- 88 nmol min-1 100 ml-1 of tissue) compared with non-exercised subjects (-460 +/- 105 nmol min-1 100 ml-1 of tissue) on day 10 of IVF. 4. Efflux of 3-methylhistidine significantly (P less than 0.05) decreased from the leg in those subjects who performed daily exercise (-0.29 +/- 0.12 nmol min-1 100 ml-1 of tissue) compared with those subjects receiving IVF without daily exercise (-1.46 +/- 0.35 nmol min-1 100 ml-1 of tissue).(ABSTRACT TRUNCATED AT 250 WORDS)
Albert
J D
JD
Department of Surgery, New York Hospital-Cornell Medical Center, New York 10021.
Matthews
D E
DE
Legaspi
A
A
Tracey
K J
KJ
Jeevanandam
M
M
Brennan
M F
MF
Lowry
S F
SF
D000328:Adult; D000596:Amino Acids / Q000378:metabolism*; D015444:Exercise; D005542:Forearm; D005998:Glycine / Q000378:metabolism; D006801:Humans; D007866:Leg; D007930:Leucine / Q000378:metabolism; D008297:Male; D010288:Parenteral Nutrition
D000596:Amino Acids; D007930:Leucine; D005998:Glycine