title: Exocytotic enzyme release from rabbit polymorphonuclear leukocytes after treatment with fluoride and calcium.
abstract: Treatment of rabbit polymorphonuclear leukocytes (PMNs) with 20 mM sodium fluoride for 10 min, followed by removal of fluoride and addition of Ca2+ results in extensive exocytosis. This is apparent from a strong lysozyme release, together with a slight LDH release. During fluoride-activated Ca2+-dependent exocytosis an increase of indo fluorescence and a strong association of 45Ca with the cells occurs. Different inhibitors inhibit both 45Ca association and lysozyme release. Pretreatment of PMNs with pertussis toxin, or the presence of AI3+ in the medium has little effect on fluoride-activated Ca2+-dependent exocytosis. During pretreatment with fluoride, the ATP level strongly decreases. Exocytosis nevertheless occurs upon addition of Ca2+, indicating that a normal ATP level is not required for exocytosis. The glycogen content of the cell strongly decreases during exposure to Ca2+ after pretreatment with fluoride, but not during pretreatment with fluoride. Breakdown of glycogen and accumulation of 3-phosphoglycerate suggest that glycolysis is blocked at the enolase step, but proceeds as far as that step.
authors.affiliation: Department of Medical Biochemistry, Sylvius Laboratories, University of Leiden, The Netherlands.
authors.forename: J G
authors.initials: JG
authors.lastname: Elferink
authors.forename: M
authors.initials: M
authors.lastname: Deierkauf
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology*; D002132:Calcium Radioisotopes; D005089:Exocytosis / Q000187:drug effects / Q000502:physiology*; D005988:Glyceric Acids / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D006019:Glycolysis; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009113:Muramidase / Q000378:metabolism*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology*; D011817:Rabbits; D012969:Sodium Fluoride / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D002132:Calcium Radioisotopes; D005988:Glyceric Acids; D000001:Calcimycin; D009240:N-Formylmethionine Leucyl-Phenylalanine; C005156:3-phosphoglycerate; D000255:Adenosine Triphosphate; D012969:Sodium Fluoride; D006003:Glycogen; D007770:L-Lactate Dehydrogenase; D009113:Muramidase; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium