Blocking of passive sensitization of human mast cells and basophil granulocytes with IgE antibodies by a recombinant human epsilon-chain fragment of 76 amino acids.
The recombinant peptide corresponding to residues 301-376 at the junction of constant regions 2 and 3 of the human IgE epsilon chain blocked the in vivo passive sensitization of human skin mast cells and in vitro sensitization of human basophil granulocytes with human IgE antibodies. An injection of the recombinant peptide or E myeloma protein into normal skin sites 1 hr before sensitization with an allergic serum blocked passive sensitization. In this system, approximately 10-fold higher molar concentration of the recombinant peptide than E myeloma protein was required for 50% inhibition of Prausnitz-Küstner reactions. When the mononuclear cells of two normal individuals were preincubated with the recombinant peptide or E myeloma protein for 15 min before passive sensitization with the same allergic serum and the cells were challenged with an optimal concentration of an antigen, approximately 11- to 13-fold higher concentration of the recombinant peptide than E myeloma protein was required for 50% inhibition of antigen-induced histamine release. Further studies with several recombinant peptides indicated that amino acid resides 363-376 in the Fc epsilon-chain fragment are not essential for binding of the peptide to Fc epsilon-chain receptor I.
Helm
B
B
Division of Molecular Biology and Biotechnology/Biochemistry, Sheffield University, England.
Kebo
D
D
Vercelli
D
D
Glovsky
M M
MM
Gould
H
H
Ishizaka
K
K
Geha
R
R
Ishizaka
T
T
D001483:Base Sequence; D001491:Basophils / Q000276:immunology*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D007116:Immunization, Passive; D007073:Immunoglobulin E / Q000276:immunology*; D007143:Immunoglobulin Heavy Chains / Q000276:immunology*; D007139:Immunoglobulin epsilon-Chains / Q000235:genetics / Q000276:immunology*; D008407:Mast Cells / Q000276:immunology*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins / Q000276:immunology
D007143:Immunoglobulin Heavy Chains; D007139:Immunoglobulin epsilon-Chains; D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins; D007073:Immunoglobulin E; D004247:DNA