title: Co-existence of TRH with mesotocin in the same axon terminals of the bullfrog pars nervosa as revealed by double labeling immunocytochemistry.
abstract: The co-existence of thyrotropin-releasing hormone (TRH)- and mesotocin (MT)-like immunoreactivities in single axon terminals in the frog pars nervosa was observed using a method combining pre-embedding peroxidase-antiperoxidase for TRH with post-embedding immunocolloidal gold staining for MT. Both TRH- and MT-like immunoreactivities were localized in the same large dense elementary granules 130-220 nm in diameter (170 nm mean diameter). A few axons contained only TRH-like immunoreactive small granular vesicles 80-120 nm in diameter (92 nm mean diameter). Axon terminals containing both TRH- and MT-like immunoreactive granules were in direct contact with the perivascular basal lamina of blood capillaries.
authors.affiliation: Department of Anatomy, Showa University School of Medicine, Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Shioda
authors.forename: Y
authors.initials: Y
authors.lastname: Nakai
authors.forename: C
authors.initials: C
authors.lastname: Iwai
authors.forename: H
authors.initials: H
authors.lastname: Sunayama
mesh_terms: D000818:Animals; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D009411:Nerve Endings / Q000378:metabolism* / Q000648:ultrastructure; D010121:Oxytocin / Q000031:analogs & derivatives* / Q000378:metabolism; D010904:Pituitary Gland, Posterior / Q000378:metabolism* / Q000648:ultrastructure; D011892:Rana catesbeiana / Q000033:anatomy & histology / Q000378:metabolism*; D013973:Thyrotropin-Releasing Hormone / Q000378:metabolism*
chemical_list: C001713:mesotocin; D010121:Oxytocin; D013973:Thyrotropin-Releasing Hormone