Cytochemical study of intracellular calcium in hamster spermatozoa during the acrosome reaction.
Calcium was localized by a pyroantimonate technique in hamster spermatozoa during the acrosome reaction and pyroantimonate precipitates were observed in the anterior region of the acrosome. The calcium was also localized in the postacrosomal lamina of spermatozoa undergoing the acrosome reaction. Spermatozoa, incubated in capacitating medium containing verapamil, showed denser precipitates with an increase in concentration of this drug. Ionophore A23187 enhanced binding of calcium to the acrosomal region. The sodium channel inhibitor amiloride inhibited the acrosome reaction and the pyroantimonate precipitates were absent in these spermatozoa, whereas ionophore monensin enhanced the acrosome reaction. This suggests that the Na+/Ca++ antiporter may be responsible for intracellular Ca++ regulation during the acrosome reaction in hamster spermatozoa.
Ruknudin
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Laboratory of Histology, Embryology and Cytogenetics, University of Paris VI, Faculty of Medicine, France.
D000177:Acrosome / Q000378:metabolism*; D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D000965:Antimony; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D006224:Cricetinae; D008297:Male; D008985:Monensin / Q000494:pharmacology; D012964:Sodium / Q000378:metabolism; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000378:metabolism*; D014700:Verapamil / Q000494:pharmacology
C009002:potassium antimonate; D000001:Calcimycin; D000584:Amiloride; D008985:Monensin; D000965:Antimony; D012964:Sodium; D014700:Verapamil; D002118:Calcium