title: [Study of fluorescence in situ hybridization for detection of chromosome aberration].
abstract: The authors applied fluorescence in situ hybridization (FISH) technique for the detection of chromosome aberration in interphase nuclei using the probe specific to alphoid repeats on chromosome 11 and X. Chromosome 11 specific probe showed two major spots in lymphocyte nuclei, while X specific probe showed single spot in male and double spots in female respectively. On the other hand three spots were detected in most of the nuclei from HeLa cells with 11 and X specific probes. We concluded that FISH with the use of chromosome specific probe may become a useful and reliable tool for the detection of chromosome aberration in interphase nuclei.
authors.affiliation: First Department of Surgery, Nagasaki University School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yasutake
authors.forename: Y
authors.initials: Y
authors.lastname: Tagawa
authors.forename: K
authors.initials: K
authors.lastname: Miyashita
authors.forename: H
authors.initials: H
authors.lastname: Ishikawa
authors.forename: S
authors.initials: S
authors.lastname: Hara
authors.forename: D
authors.initials: D
authors.lastname: Okada
authors.forename: K
authors.initials: K
authors.lastname: Kuwahara
authors.forename: N
authors.initials: N
authors.lastname: Kawazoe
authors.forename: H
authors.initials: H
authors.lastname: Yamaguchi
authors.forename: M
authors.initials: M
authors.lastname: Tomita
mesh_terms: D002467:Cell Nucleus; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D002880:Chromosomes, Human, Pair 11; D015342:DNA Probes; D005453:Fluorescence; D006367:HeLa Cells; D006801:Humans; D007399:Interphase; D008214:Lymphocytes; D009693:Nucleic Acid Hybridization; D014960:X Chromosome
chemical_list: D015342:DNA Probes