title: Construction, stable transformation, and function of an amber suppressor tRNA gene in Drosophila melanogaster.
abstract: Drosophila melanogaster strains with a stably incorporated amber suppressor tRNA gene have been generated. A tRNATyr gene was site specifically mutated to produce an anticodon sequence that recognizes the amber codon and then introduced into Drosophila by using P-element-mediated transformation. Transformants from four integration events were recovered. Two integrations resulted in both male and female sterility, whereas the other two resulted in male sterility but female fertility. Strains derived from the two female-fertile integration events were shown to have a low level of amber-suppressing activity by their ability to suppress an amber mutation in a chloramphenicol acetyltransferase gene.
authors.affiliation: Department of Biochemistry, University of California, Berkeley 94720.
authors.forename: F A
authors.initials: FA
authors.lastname: Laski
authors.forename: S
authors.initials: S
authors.lastname: Ganguly
authors.forename: P A
authors.initials: PA
authors.lastname: Sharp
authors.forename: U L
authors.initials: UL
authors.lastname: RajBhandary
authors.forename: G M
authors.initials: GM
authors.lastname: Rubin
mesh_terms: D000818:Animals; D001483:Base Sequence; D004331:Drosophila melanogaster / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D012345:RNA, Transfer, Amino Acid-Specific / Q000235:genetics*; D012365:RNA, Transfer, Tyr / Q000235:genetics*; D013489:Suppression, Genetic; D014170:Transformation, Genetic
chemical_list: D012345:RNA, Transfer, Amino Acid-Specific; D012365:RNA, Transfer, Tyr