Participation of the membrane effect to the cytotoxicity of tensio-active agents comparison with ocular irritation in vivo.
A study for the comparison of the action of tensio-active agents have been made with 3 models: The rabbit erythrocyte to evaluate the immediate membrane effect. SIRC fibroblasts (rabbit cornea) to evaluate the toxicity after 3 days. The rabbit eye (Draize). The cellular model have shown that there is a difference between non irritants tensio-active agents, and irritants-very irritants in vivo, except the BRIJ group. To evaluate the part of membrane effect in the interaction tensio-active-cell, it has been realized a test of haemolysis on the rabbit erythrocyte on 10 tensio-active agents. It shows that the cellular model can also determine a different toxicity than the one provoked by an aggression on the plasmic membrane.
Conduzorgues
J
J
Hagege
M
M
Dumas
M
M
Meybeck
A
A
D000818:Animals; D003228:Conjunctiva / Q000187:drug effects; D004342:Drug Hypersensitivity; D004910:Erythrocyte Membrane / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D066298:In Vitro Techniques; D011817:Rabbits; D013501:Surface-Active Agents / Q000633:toxicity*; D014116:Toxicology / Q000379:methods*
D013501:Surface-Active Agents