[The effect of space flight conditions on the rate of multiplication, morphology of cells, DNA and protein content in the ciliate Tetrahymena pyriformis].
The conditions of a space flight and, in particular, the weightlessness promote an increased density of the ciliate culture, an enhanced reproduction rate, and an elevated ratio of dividing cells. The condition of weightlessness brings about some decrease in the bulk protein content of the cells determined by cytophotometry of Naphthol-yellow stained ciliates. The quantity of DNA in macronuclei was measured following the routine Feulgen procedure (its "cold" variant). The DNA content was found to remain unchanged. Some changes in the shape and size of the cells were noticed under flight conditions: ciliates that had developed in weightlessness appeared more spherical than control ones, due presumably to a decrease in the body length and to some extension in the body width. The conditions of space flight, including the weightlessness, induce changes in the physiological status of unicellular organisms. A decrease in the gravitation force may lead to a decrease in the energy expenditures for maintenance of the cell positional homeostasis.
Prlina
I S
IS
Gabova
A V
AV
Raĭkov
I B
IB
Tairbekov
M G
MG
D000818:Animals; D002455:Cell Division; D004247:DNA / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D013026:Space Flight; D013769:Tetrahymena pyriformis / Q000737:chemistry* / Q000166:cytology; D014893:Weightlessness
D011506:Proteins; D004247:DNA
Vliianie usloviĭ kosmicheskogo poleta na skorost' razmnozheniia, morfologiiu kletok, soderzhanie DNK i belka u infuzoriĭ Tetrahymena pyriformis.