Expression of rubella virus cDNA encoding the E1 structural protein.
cDNAs synthesized from the 40S RNA genome of rubella virus were cloned into the lambda gt10 bacteriophage. A clone (pRV # 1475) which contains a truncated version of the E1 envelope glycoprotein (amino acid residues 17-481) and the 3' non-coding region was constructed from two overlapping cDNAs. pRV # 1475 was inserted into a eukaryotic expression vector under the control of the cytomegalovirus immediate early promoter. After transfection of 293 cells, a protein with intact antigenic domains could be detected.
de Mazancourt
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A
Institut Gustave Roussy, Villejuif, France.
Perricaudet
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M
D002460:Cell Line; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000096:biosynthesis* / Q000235:genetics; D005455:Fluorescent Antibody Technique; D010957:Plasmids; D012367:RNA, Viral / Q000302:isolation & purification; D012412:Rubella virus / Q000235:genetics*; D014162:Transfection; D014759:Viral Envelope Proteins / Q000096:biosynthesis* / Q000235:genetics
D004279:DNA, Viral; D012367:RNA, Viral; D014759:Viral Envelope Proteins