Tissue distribution of beta 1- and beta 2-subunits of regulatory guanine nucleotide-binding proteins.
The beta-subunit of G-proteins occurs in two forms (beta 1 and beta 2), which differ in their primary structure as derived from cDNA clones and in their mobilities on SDS gels (36 and 35 kDa, respectively). To assess the tissue distribution of the two forms of beta-subunits, we synthesized peptides corresponding to defined regions of beta 1- and beta 2-subunits and injected them into rabbits; the antisera obtained reacted either with both beta-subunits or specifically with the beta 1- or the beta 2-subunit. They were used to identify the two beta-subunits in membranes prepared from various rat tissues and from human placenta. The concentration of total beta-subunits was high in rat brain and lung, human placenta, rat kidney, liver and spleen; it was much lower in rat erythrocytes, cardiac and skeletal muscle. In all tissues studied, both beta 1- and beta 2-subunits were detectable. In most tested tissues, the two forms were about equally distributed, whereas in the placenta, the beta 2-subunit was found to occur in approx. 2-fold excess over the beta 1-subunit. Our results demonstrate that both beta-subunits are widely distributed. In the majority of tissues, levels of beta 2-subunits are very similar to those of beta 1-subunits. Thus, the abundance of beta 2-subunits as compared to that of the beta 1-subunit is considerably higher than was previously estimated by measuring the respective mRNA levels.
Hinsch
K D
KD
Institut für Pharmakologie, Freie Universität Berlin, F.R.G.
Tychowiecka
I
I
Gausepohl
H
H
Frank
R
R
Rosenthal
W
W
Schultz
G
G
D000595:Amino Acid Sequence; D000818:Animals; D000936:Antigen-Antibody Complex; D002462:Cell Membrane / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D019204:GTP-Binding Proteins / Q000032:analysis* / Q000276:immunology; D007106:Immune Sera; D015151:Immunoblotting; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011247:Pregnancy; D051381:Rats
D000936:Antigen-Antibody Complex; D007106:Immune Sera; D046911:Macromolecular Substances; D019204:GTP-Binding Proteins