Cyanase-mediated utilization of cyanate in Pseudomonas fluorescens NCIB 11764.
Pseudomonas fluorescens NCIB 11764 was capable of utilizing cyanate (OCN-) as a sole nitrogen source for growth. Crude cell extracts from cells grown on cyanate, but not on ammonium sulfate, were induced for an enzyme catalyzing cyanate conversion to ammonia. Enzymatic activity was shown to be bicarbonate dependent and specific for cyanate as a substrate, suggesting that cyanate utilization in this organism is facilitated by an enzyme resembling cyanase (cyanate amidohydrolase; EC 3.5.5.3), as described previously in Escherichia coli and Flavobacterium sp.
Kunz
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Department of Biological Sciences, University of North Texas, Denton 76203-5218.
Nagappan
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D000619:Aminohydrolases / Q000378:metabolism*; D000641:Ammonia / Q000378:metabolism; D019759:Carbon-Nitrogen Lyases; D003470:Culture Media; D003485:Cyanates / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D011551:Pseudomonas fluorescens / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism
D003470:Culture Media; D003485:Cyanates; D000641:Ammonia; D000619:Aminohydrolases; C019953:cyanate hydrolase; D019759:Carbon-Nitrogen Lyases