title: [The role of phosphorylase kinase subunits in interaction with glycogen].
abstract: The interaction of rabbit skeletal muscle phosphorylase kinase with CNBr-activated glycogen results in the formation of a covalent complex. The non-bound kinase was removed by chromatography on DEAE-cellulose and phenyl-Sepharose. The amount of the bound protein increased with an increase in the number of activated groups in the glycogen molecule; the enzyme activity was thereby decreased. The kinase covalently and non-covalently bound to glycogen exhibited a higher affinity for the protein substrate (phosphorylase b) as well as for Mg2+ and Ca2+ than did the kinase in the absence of glycogen. Electrophoresis performed under denaturating conditions showed that the gamma-subunit of phosphorylase kinase is responsible for the enzyme binding to CNBr-glycogen. The effect of cross-linking reagents (glutaric aldehyde, 1.5-difluoro-2.4-dinitrobenzene) on the binding of phosphorylase kinase subunits was studied. Glycogen afforded protection of the gamma-subunit from the cross-linking to other enzyme subunits. An analysis of the subunit composition of phosphorylase kinase covalently bound to CNBr-glycogen and of the enzyme treated with cross-linking reagents in the presence of glycogen-revealed that the gamma-subunit is involved in the specific binding of phosphorylase kinase to glycogen.
authors.forename: S A
authors.initials: SA
authors.lastname: Shur
authors.forename: M A
authors.initials: MA
authors.lastname: Zemskova
authors.forename: P L
authors.initials: PL
authors.lastname: Vulfson
authors.forename: L K
authors.initials: LK
authors.lastname: Skolysheva
mesh_terms: D000818:Animals; D003488:Cyanogen Bromide; D004591:Electrophoresis, Polyacrylamide Gel; D005976:Glutaral / Q000494:pharmacology; D006003:Glycogen / Q000378:metabolism*; D007202:Indicators and Reagents; D007700:Kinetics; D009132:Muscles / Q000378:metabolism; D010764:Phosphorylase Kinase / Q000378:metabolism*; D011817:Rabbits
chemical_list: D007202:Indicators and Reagents; D006003:Glycogen; D010764:Phosphorylase Kinase; D003488:Cyanogen Bromide; D005976:Glutaral
vernacular_title: Rol' sub''edinits kinazy fosforilazy vo vzaimodeĭstii s glikogenom.