Calcium rise in human platelets elicited by anti-CD9 and -CD41 murine monoclonal antibodies.
Three murine monoclonal antibodies (anti-CD9: ALB6, anti-CD41: VI-PL3 and PL2-49/GPIIb - final concentration: 7.5 micrograms/mL) are shown to elicit after a lag time aggregation of washed platelets and a calcium signal (as detected by light emitted by loaded aequorin), which is only partially inhibited by aspirin. By comparison the rise induced by thrombin is greater and almost instantaneous. In the presence of EGTA a calcium mobilization from internal stores can be detected with thrombin and with ALB6, but neither with PL2-49 nor with VI-PL3, whereas platelets still change their shape and release ATP. It is tempting to speculate that although all the antibodies induce a calcium change, they activate platelets by different pathways: calcium may be not primarily involved in the activation induced by the anti-CD41 antibodies.
Favier
R
R
Laboratoire Central d'Hématologie, Hôtel Dieu, Paris, France.
Lecompte
T
T
Morel
M C
MC
Potevin
F
F
Benoit
P
P
Boucheix
C
C
Kaplan
C
C
Samama
M
M
D000255:Adenosine Triphosphate / Q000378:metabolism; D000331:Aequorin / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000943:Antigens, Differentiation / Q000276:immunology*; D001241:Aspirin / Q000494:pharmacology; D001693:Biological Transport, Active / Q000187:drug effects; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D002118:Calcium / Q000032:analysis*; D004533:Egtazic Acid / Q000494:pharmacology; D006801:Humans; D051379:Mice; D010974:Platelet Aggregation / Q000187:drug effects; D010980:Platelet Membrane Glycoproteins / Q000276:immunology* / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013917:Thrombin / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D010980:Platelet Membrane Glycoproteins; D000331:Aequorin; D004533:Egtazic Acid; D000255:Adenosine Triphosphate; D013917:Thrombin; D001241:Aspirin; D002118:Calcium