Mureidomycins A-D, novel peptidylnucleoside antibiotics with spheroplast forming activity. I. Taxonomy, fermentation, isolation and physico-chemical properties.
A strain of actinomycetes identified as Streptomyces flavidovirens produced new antibiotics, mureidomycins (MRD's) A approximately D, specifically active against Pseudomonas aeruginosa. They were isolated from the culture filtrate by successive column chromatographies such as Amberlite XAD-2 and CG-50, Whatman DE-52 and Toyopearl HW-40. They were amphoteric white powders and soluble in methanol and water. Their molecular weights and molecular formulae in parentheses were 840 (C38H48N8O12S), 842 (C38H50N12S), 897 (C40H51N9O13S) and 899 (C40H53N9O13S), respectively. m-Tyrosine and two unknown substances were detected by amino acid analyses as their common constituents. MRD's A and C contained uracil but MRD's B and D dihydrouracil instead of uracil.
Inukai
M
M
Fermentation Research Laboratories, Sankyo Co., Ltd., Tokyo, Japan.
Isono
F
F
Takahashi
S
S
Enokita
R
R
Sakaida
Y
Y
Haneishi
T
T
D000596:Amino Acids / Q000032:analysis; D000900:Anti-Bacterial Agents; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002845:Chromatography; D005285:Fermentation; D013058:Mass Spectrometry; D000432:Methanol; D008970:Molecular Weight; D009705:Nucleosides; D010455:Peptides; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012995:Solubility; D013104:Spheroplasts / Q000187:drug effects*; D013302:Streptomyces / Q000145:classification* / Q000166:cytology / Q000502:physiology; D014498:Uracil / Q000032:analysis; D014867:Water
D000596:Amino Acids; D000900:Anti-Bacterial Agents; D009705:Nucleosides; D010455:Peptides; D014867:Water; C059803:mureidomycin A; C059804:mureidomycin B; C059805:mureidomycin C; C059806:mureidomycin D; D014498:Uracil; D000432:Methanol