Temperature-sensitive gel for virus concentration from urine.
Cross-linked, partially hydrolyzed polyacrylamide gels (temperature-sensitive gels) with the property to swell at 4 degrees C and collapse at higher temperatures (greater than 19 degrees C) were used to concentrate bacteriophage T-2 from urine. Samples of urine, 50 ml, seeded with bacteriophage T-2 were reduced to approximately 5 ml, with an average virus recovery of 53%. Subsequent experiments with feline cell-associated herpes virus resulted in a 6-fold decrease in volume with 54% virus recovery. The gel could be used repeatedly without any loss in efficiency.
Maheshkumar
S
S
Department of Veterinary Diagnostic Investigation, College of Veterinary Medicine, University of Minnesota, St. Paul 55108.
Peterson
R B
RB
Goyal
S M
SM
D000180:Acrylic Resins; D000818:Animals; D002415:Cats; D006564:Herpesviridae / Q000302:isolation & purification; D006801:Humans; D013604:T-Phages / Q000302:isolation & purification; D013696:Temperature; D014556:Urine / Q000382:microbiology*; D014773:Virology / Q000379:methods*; D014777:Virus Diseases / Q000175:diagnosis / Q000382:microbiology / Q000652:urine; D014780:Viruses / Q000302:isolation & purification*
D000180:Acrylic Resins; C016680:polyacrylamide gels