Improved separation of human pepsins from gastric juice by high-performance ion-exchange chromatography.
A simple and precise separative procedure can now be used to isolate individual pepsins from gastric juice, and by measurement of the protein absorbance at 280 nm enable their direct quantitation. This will facilitate the study of pepsin secretion, particularly in patients with peptic ulcer disease.
Peek
K
K
Department of Chemical Pathology, Royal Liverpool Hospital, U.K.
Roberts
N B
NB
Taylor
W H
WH
D002852:Chromatography, Ion Exchange; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005750:Gastric Juice / Q000201:enzymology*; D006801:Humans; D006868:Hydrolysis; D010418:Pentagastrin / Q000494:pharmacology; D010434:Pepsin A / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
D010434:Pepsin A; D010418:Pentagastrin