Comparison of dot blot with in-situ hybridization for the detection of Neisseria gonorrhoeae in urethral exudate.
Dot blot hybridization and in-situ hybridization with DNA probes prepared from total genomic gonococcal DNA were compared with Gram staining and culturing for the detection of gonococci in urethral exudates. Fifteen of 60 patients were positive by at least one of the four methods. Gram staining and in-situ hybridization scored best with 13 positives but culture and dot blot hybridization yielded only 10 and 9 positives respectively. The failure to detect gonococci in culture can be explained by overgrowth in one case and possibly self medication with ampicillin before culture in another. The in-situ hybridization test is a fast and sensitive hybridization method for the detection of gonococci in urethral exudate from men.
Schoone
G J
GJ
N.H. Swellengrebel Laboratory of Tropical Hygiene, Royal Tropical Institute, Amsterdam, The Netherlands.
Cornelissen
W J
WJ
Veenhuijsen
P C
PC
Schöneman
C E
CE
Terpstra
W J
WJ
D003429:Cross Reactions; D004269:DNA, Bacterial / Q000032:analysis*; D006069:Gonorrhea / Q000175:diagnosis*; D006801:Humans; D008297:Male; D009344:Neisseria gonorrhoeae / Q000235:genetics / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D011237:Predictive Value of Tests; D014521:Urethra / Q000382:microbiology*
D004269:DNA, Bacterial