Phosphorus-31 nuclear magnetic resonance spectroscopy of toad retina.
Phosphorus-31 nuclear magnetic resonance (31P-NMR) spectra were obtained from living toad retinae and toad retinal extracts at 4 degrees C. Several phosphorus metabolites--nucleoside di- and triphosphates (NTP), phosphocreatine, phosphodiesters, inorganic phosphate, and phosphomonoesters--were identified from the spectra of whole retinae. The intracellular pH was determined to be 7.27 +/- 0.06 at 4 degrees C and the intracellular MgNTP/NTP ratio was at least 0.77. These results are consistent with those reported by other techniques, and they show that 31P-NMR spectroscopy can be used for noninvasively and quantitatively studying the metabolism of living toad retinae, and for monitoring its changes over time.
Apte
D V
DV
Department of Physiology and Biophysics, University of Illinois, Urbana 61801.
Koutalos
Y
Y
McFarlane
D K
DK
Dawson
M J
MJ
Ebrey
T G
TG
D000818:Animals; D002024:Bufo marinus; D005612:Freeze Drying; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000032:analysis / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D010759:Phosphorus Isotopes; D012160:Retina / Q000032:analysis*
D010759:Phosphorus Isotopes; D008274:Magnesium