Mechanical stimulation by intermittent compression stimulates sulfate incorporation and matrix mineralization in fetal mouse long-bone rudiments under serum-free conditions.
Mechanical stimulation evoked by intermittent hydrostatic compression (IC) in a closed culture system has been shown to stimulate calcification of fetal long-bone rudiments in the presence of serum [6]. We have studied effects of IC on sulfate metabolism and matrix mineralization under serum-free conditions, in short-term (24 hours) cultures of mineralizing long-bone rudiments in alpha minimum essential medium (MEM) + 0.2% bovine serum albumen (BSA). Exposure to IC for 24 hours stimulated radiosulfate incorporation into the papain-digestible pool in the noncalcifying epiphyses and, to a larger extent, in the calcifying diaphysis. The percentage release of 35S from prelabeled rudiments was stimulated in the epiphyses, but inhibited in the diaphyses. The changes in sulfate metabolism of matrix mineralization, in hypertrophic cartilage, and the diaphyseal bone collar, were judged from the increase in length of the diaphysis. This study shows that under serum-free conditions, mechanical stimulation by IC increases sulfate content while stimulating mineralization in calcifying cartilage of fetal long-bone rudiments. Mechanical stimulation seems to be an important regulator of cartilage calcification.
Bagi
C
C
Department of Oral Cell Biology, Akademisch Centrum Tandheelkunde Amsterdam-Vrije Universiteit, The Netherlands.
Burger
E H
EH
D000818:Animals; D001857:Bone Matrix / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D001842:Bone and Bones / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D002113:Calcification, Physiologic / Q000187:drug effects / Q000502:physiology*; D003470:Culture Media / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D009924:Organ Culture Techniques; D010812:Physical Stimulation; D013314:Stress, Mechanical; D013431:Sulfates / Q000378:metabolism*
D003470:Culture Media; D013431:Sulfates