title: Ovarian transforming growth factor-beta (TGF beta): cellular site(s), and mechanism(s) of action.
abstract: It is the objective of the experiments reported herein to examine the possible relevance of transforming growth factor-beta (TGF beta) to theca-interstitial cell function, and to further characterize the established interaction of TGF beta with the granulosa cell. In examining the interaction of TGF beta (10 ng/ml) with murine theca-interstitial cells, no significant effect was observed on either basal or human chorionic gonadotropin (hCG)-stimulated androsterone accumulation. In contrast, given murine granulosa cells, TGF beta (10 ng/ml) produced dose- and time-dependent augmentation of follicle-stimulating hormone (FSH)-supported aromatase activity with a minimal and median effective doses of 20 +/- 3 and 123 +/- 24 pg/ml, respectively and a minimal time requirement of less than or equal to 48 h. The ability of TGF beta to augment FSH hormonal action could not be accounted for by alteration(s) of specific FSH binding (13965 +/- 298 and 12614 +/- 694 cpm/4 X 10(5) cells for FSH and FSH + TGF beta). However, TGF beta proved capable of exerting a direct upregulatory effect on stimulatable adenylate cyclase activity, further enhancement occurring at site(s) distal to cAMP generation (dibutyryl cyclic AMP (Bt2cAMP) = 1.4 +/- 0.2 ng/culture; Bt2cAMP + TGF beta = 4.1 +/- 0.6 ng/culture). Taken together, our findings are in keeping with the notion that TGF beta, possibly of intraovarian origin, comprises the central signal of autocrine or paracrine loop(s) capable of amplifying gonadotropin action at the level of the granulosa, but not theca-interstitial cell.
authors.affiliation: Department of Obstetrics/Gynecology, University of Maryland School of Medicine, Baltimore 21201.
authors.forename: E Y
authors.initials: EY
authors.lastname: Adashi
authors.forename: C E
authors.initials: CE
authors.lastname: Resnick
authors.forename: E R
authors.initials: ER
authors.lastname: Hernandez
authors.forename: J V
authors.initials: JV
authors.lastname: May
authors.forename: A F
authors.initials: AF
authors.lastname: Purchio
authors.forename: D R
authors.initials: DR
authors.lastname: Twardzik
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D001141:Aromatase / Q000378:metabolism; D002478:Cells, Cultured; D005576:Colforsin / Q000494:pharmacology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006107:Granulosa Cells / Q000187:drug effects* / Q000201:enzymology; D010053:Ovary / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D013799:Theca Cells / Q000187:drug effects* / Q000378:metabolism; D015291:Transforming Growth Factors / Q000494:pharmacology*
chemical_list: D005576:Colforsin; D015291:Transforming Growth Factors; D005640:Follicle Stimulating Hormone; D001141:Aromatase; D000262:Adenylyl Cyclases