Monoadduct forming photochemical reagents for labeling nucleic acids for hybridization.
We describe the synthesis of three angelicin derivatives which can be used for labeling nucleic acids with biotin. These compounds were used to label nucleic acids in the presence of lysed cell constituents. The resulting labelled nucleic acids show hybridization to a genus specific probe for E. coli. The relative comparison of sensitivity indicates that a polyamine linker is better than a polyethylene oxide linker between the biotin and angelicin moieties.
Albarella
J P
JP
Miles Inc., Elkhart, IN 46515.
Minegar
R L
RL
Patterson
W L
WL
Dattagupta
N
N
Carlson
E
E
D000345:Affinity Labels; D001710:Biotin; D003432:Cross-Linking Reagents; D015342:DNA Probes; D013293:Enterococcus faecalis / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D011564:Furocoumarins; D007202:Indicators and Reagents; D009693:Nucleic Acid Hybridization; D010777:Photochemistry / Q000379:methods; D011073:Polyamines; D011513:Proteus mirabilis / Q000235:genetics; D012996:Solutions; D013212:Staphylococcus epidermidis / Q000235:genetics
D000345:Affinity Labels; D003432:Cross-Linking Reagents; D015342:DNA Probes; D011564:Furocoumarins; D007202:Indicators and Reagents; D011073:Polyamines; D012996:Solutions; D001710:Biotin; C011659:angelicin