Changes in mRNA levels of poly(ADP-ribose) polymerase during activation of human lymphocytes.
The level of mRNA encoding the nuclear enzyme poly(ADP-ribose) polymerase (ADP-ribosyltransferase, EC 2.4.2.30) was found to be very low in quiescent human lymphocytes and to increase at least 10-fold between 1 and 2 dyas after stimulation with the mitogen phytohaemagglutinin, staying high for several days thereafter. This increase was inhibited by 3-methoxybenzamide (a competitive inhibitor of poly(ADP-ribose) polymerase) but was not affected significantly by aphidicolin. Incubation of activated cells with cycloheximide for 2 h increased the expression slightly. These data demonstrate that, during lymphocyte activation, the level of mRNA of the poly(ADP-ribose) polymerase gene correlates with, and hence is presumably responsible for, the increase in poly(ADP-ribose) polymerase protein detectable by enzyme assay or immunochemistry.
McNerney
R
R
Department of Immunology, St George's Hospital Medical School, London, U.K.
Tavasolli
M
M
Shall
S
S
Brazinski
A
A
Johnstone
A
A
D016590:Aphidicolin; D001549:Benzamides / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D004224:Diterpenes; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007700:Kinetics; D008213:Lymphocyte Activation / Q000235:genetics*; D008214:Lymphocytes / Q000201:enzymology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis*
D001549:Benzamides; D004224:Diterpenes; D010835:Phytohemagglutinins; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D012333:RNA, Messenger; D016590:Aphidicolin; D003513:Cycloheximide; D011065:Poly(ADP-ribose) Polymerases; C025159:3-methoxybenzamide