Reduced intestinal calcium and dietary calcium intake, increased aluminum absorption, and tissue concentration in the rat.
To test the influence of calcium (Ca) on aluminum (Al) absorption, Ca was withheld from or added (1mM) to the perfusate of the in situ rat gut. The rats had been maintained on Purina Rat Chow. Ca addition significantly decreased (to 70%) the rate of Al disappearance from the gut and decreased (to 55%) the area under the curve of Al appearance in portal blood. To test the influence of Ca deficiency on Al absorption, rats were maintained on a low-Ca (0.008%) or a Ca-replete (0.5%) diet for 1-4 wk. The in situ gut was prepared, and a perfusate containing approximately 1 microM Ca was used. The rate of Al disappearance from the gut of low-Ca diet rats was significantly faster than from the gut of rats maintained on the Ca-replete diet, averaging 156% of the latter. Al appearance in portal blood was significantly greater (averaging 38%) in rats maintained on the low-Ca diet than in controls. To determine if Ca deficiency influences Al tissue distribution independent of gastrointestinal Al absorption, rats maintained on a low-Ca or a Ca-replete diet received 20 ip Al injections over 1 mo. Rats eating the low-Ca diet demonstrated enhanced tissue Al accumulation in all tissues studied, except for muscle and cerebral cortex. These results demonstrate enhanced Al absorption and tissue retention in the presence of reduced intestinal Ca concentration and reduced Ca intake.
Provan
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SD
College of Pharmacy, University of Kentucky, Lexington 40536-0082.
Yokel
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RA
D000535:Aluminum / Q000493:pharmacokinetics*; D000818:Animals; D002118:Calcium / Q000032:analysis / Q000172:deficiency / Q000378:metabolism*; D002136:Calcium, Dietary / Q000494:pharmacology*; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
D002136:Calcium, Dietary; D000535:Aluminum; D002118:Calcium