title: Consecutive diaphorase-acetylcholinesterase histochemistry in the myenteric plexus of frog stomach.
abstract: An investigation was made on the frog stomach myenteric plexus with 2 different histochemical techniques. Neuronal perikarya were stained with nicotinamide-adenine-dinucleotide-diaphorase (NADHd), while the acetyl-cholinesterase (AChE) staining showed rather the axoarchitectonic arrangement of the frog myenteric plexus. In double-labelled "whole mounts", NADHd-positive cell bodies and AChE-positive nerve processes were revealed. Some of the nerve cells and neuronal processes did not exhibit AChE activity at all. Since glyoxylic acid-induced fluorescence (GIF) was not detected in the myenteric plexus, the presence of catecholamines can be excluded. As a consequence of these observations, we suggest the presence of a non-cholinergic, non-adrenergic intrinsic neuronal system in the frog stomach myenteric plexus, containing purines or peptides as transmitters.
authors.affiliation: Department of Zoology, Attila József University, Szeged, Hungary.
authors.forename: R
authors.initials: R
authors.lastname: Gábriel
authors.forename: E
authors.initials: E
authors.lastname: Fekete
authors.forename: K
authors.initials: K
authors.lastname: Halasy
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D002395:Catecholamines / Q000032:analysis; D008058:Dihydrolipoamide Dehydrogenase / Q000378:metabolism*; D006651:Histocytochemistry; D008856:Microscopy, Fluorescence; D009197:Myenteric Plexus / Q000166:cytology / Q000201:enzymology*; D009474:Neurons / Q000201:enzymology*; D011898:Ranidae; D013270:Stomach / Q000201:enzymology
chemical_list: D002395:Catecholamines; D008058:Dihydrolipoamide Dehydrogenase; D000110:Acetylcholinesterase