Prevention of insulin resistance by environmental manipulation as young rats mature.
This study was initiated in an attempt to see if the insulin resistance associated with maturation in young rats could be prevented by environmental manipulation. Consequently, seven week-old rats were either housed in standard laboratory cages and fed a calorie-restricted diet or placed individually in exercise wheel cages and allowed to eat chow ad lib. A control group of rats was housed in standard laboratory cages from seven weeks to five months of age, and also allowed to eat chow ad lib. When studied at five months of age, the chow-fed rats weighed more (624 +/- 8 g) than either the calorie restricted (479 +/- 9 g) or exercise trained (485 +/- 13 g) rats. Insulin action was compared in the three groups by assessing the steady-state serum glucose (SSSG) and insulin (SSSI) concentrations achieved during a continuous intravenous infusion of glucose and exogenous insulin. The results of these studies indicated that SSSG concentration was significantly higher (P less than 0.001) in chow-fed rats than in the two experimental groups. Since SSSI concentrations were the same in all three groups, lower SSSG concentrations in calorie-restricted and exercise trained rats indicates that insulin-stimulated glucose uptake was preserved in these two groups as compared to the chow-fed population. In an attempt to understand why exercise training and calorie restriction prevented the development of insulin resistance, muscle glycogen synthase activity and muscle capillary density were compared in the three groups of five month-old rats.(ABSTRACT TRUNCATED AT 250 WORDS)
Santos
R F
RF
Department of Medicine, Stanford University School of Medicine, California.
Azhar
S
S
Mondon
C
C
Reaven
E
E
D000818:Animals; D002196:Capillaries / Q000187:drug effects; D004777:Environment; D005947:Glucose / Q000378:metabolism; D006006:Glycogen Synthase / Q000037:antagonists & inhibitors; D007328:Insulin / Q000494:pharmacology; D007333:Insulin Resistance; D008297:Male; D009132:Muscles / Q000166:cytology / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains
D007328:Insulin; D006006:Glycogen Synthase; D005947:Glucose