[Analysis of tumor cell surface antigens].
The cell surface analysis is a very useful tool to diagnose tumor cells, especially hematological disorders such as leukemia and lymphoma. Particularly owing to the recent spread of flow cytometry and development of monoclonal antibodies, a single color analysis has become to be used as routine clinical series at many institutions. Recently a two color analysis with fluorescein isothiocyanate (FITC) and phycoerythrin (PE) has become to be easily applied to a cell surface staining and a more detail analysis has become to be available in studying cell surface antigens. By using a two color technic, it has been found that adult T cell leukemia cells, which are positive for CD4 (helper/inducer T lymphocytes) in a single color analysis, are derived from suppressor inducer T cells (CD4+--Leu 8+). The increased cells in thymoma have also been identified to be positive for CD4 and CD8 simultaneously (CD4+--CD8+). We conclude that a two color analysis is a very useful tool to study more detail and precise cell surface markers. A flow chart to diagnose hematological disorders with cell surface markers is also proposed in this article.
Nakahara
K
K
Dept. of Laboratory Medicine, Faculty of Medicine, University of Tokyo.
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D000951:Antigens, Neoplasm / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D016827:CD8 Antigens; D005434:Flow Cytometry; D006801:Humans; D015458:Leukemia, T-Cell / Q000276:immunology; D009369:Neoplasms / Q000175:diagnosis / Q000276:immunology*; D013945:Thymoma / Q000276:immunology; D013953:Thymus Neoplasms / Q000276:immunology
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D016827:CD8 Antigens