title: Phenylacetic acid transport system in Penicillium chrysogenum Wis 54-1255: molecular specificity of its induction.
abstract: The phenylacetic acid (PA) transport system of Penicillium chrysogenum is induced by PA, 2-hydroxyphenylacetic and 4-phenylbutyric acids but not by benzoic, phenoxyacetic acid and phenylpropionic acids. Substitution in the aromatic moiety (3-hydroxyphenylacetic, 4-hydroxyphenylacetic acids), replacement of the aromatic moiety by other rings (thiophene-2-acetic acid, indole-3-acetic or indole-3-butyric acids) or the presence of an amino group in the alpha-position (2-aminophenylacetic acid) eliminates inducing activity. 2-Phenylbutyric acid dose not induce the PA transport system indicating that fatty acid-beta-oxidation is needed to generate the authentic regulatory molecule (phenylacetyl-CoA) from 4-phenylbutyric acid. Furthermore, the uptake system synthesized in presence of PA, 2-hydroxyphenylacetic or 4-phenylbutyric acids is under carbon catabolic repression control and is also repressed by L-lysine suggesting that the three molecules induce in P. chrysogenum a single mechanism of transport.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de León, España.
authors.forename: J M
authors.initials: JM
authors.lastname: Fernández-Cañón
authors.forename: A
authors.initials: A
authors.lastname: Reglero
authors.forename: H
authors.initials: H
authors.lastname: Martínez-Blanco
authors.forename: M A
authors.initials: MA
authors.lastname: Ferrero
authors.forename: J M
authors.initials: JM
authors.lastname: Luengo
mesh_terms: D001565:Benzoates / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D010400:Penicillin G / Q000032:analysis / Q000378:metabolism; D010407:Penicillium / Q000378:metabolism*; D010408:Penicillium chrysogenum / Q000378:metabolism*; D010648:Phenylacetates / Q000378:metabolism*; D010654:Phenylbutyrates / Q000494:pharmacology; D010666:Phenylpropionates / Q000494:pharmacology; D013329:Structure-Activity Relationship
chemical_list: D001565:Benzoates; D010648:Phenylacetates; D010654:Phenylbutyrates; D010666:Phenylpropionates; C025136:phenylacetic acid; D010400:Penicillin G