The formation of biologically active beta-galactosidase inclusion bodies in Escherichia coli.
Culture conditions affecting the formation of beta-galactosidase inclusion bodies in E. coli were examined. High temperature, early induction, high salt concentration and low aeration were all found to favour an increase of insoluble beta-galactosidase and the formation of visible inclusion bodies. The ratio of soluble to insoluble beta-galactosidase decreased during the course of cell growth. When assayed for beta-galactosidase activity, the inclusion bodies were enzymatically active with a specific activity of one third that of soluble beta-galactosidase. The activity remained associated with the inclusion bodies on washing with detergent and high ionic strength buffers. These results suggest that inclusion bodies can contain correctly folded protein.
Worrall
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DM
Biotechnology Australia Pty Ltd.
Goss
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NH
D001431:Bacteriological Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology* / Q000648:ultrastructure; D001616:beta-Galactosidase / Q000096:biosynthesis*
D001616:beta-Galactosidase