Arachidonic acid metabolism by perfused ram testis.
1. Arachidonic acid was metabolized by lipoxygenase and prostaglandin synthetase enzymes systems in the perfused ram testis. 2. The major product of the prostaglandin synthetase was 6-keto-PGF1 alpha (6KF). 3. Addition of testosterone resulted in a significant increase in the 6KF. 4. Arachidonic acid (AA) as well as testosterone penetrated the perfused testis. 5. Both 15-HPETE and 15-HETE, the products of the 15-lipoxygenase enzyme, were detected. 6. Addition of 0.1% BSA changed the pattern of the oxidized arachidonic acid metabolism.
Schön
I
I
Department of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel.
Sofer
Y
Y
Cojacaru
M
M
Grossman
S
S
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis; D066298:In Vitro Techniques; D015289:Leukotrienes / Q000096:biosynthesis; D008054:Lipid Peroxides / Q000096:biosynthesis; D008084:Lipoxygenase / Q000378:metabolism; D008297:Male; D010084:Oxidation-Reduction; D010477:Perfusion; D011453:Prostaglandins / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D012756:Sheep; D013737:Testis / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D013739:Testosterone / Q000494:pharmacology
D001095:Arachidonic Acids; D006893:Hydroxyeicosatetraenoic Acids; D015289:Leukotrienes; D008054:Lipid Peroxides; D011453:Prostaglandins; D012710:Serum Albumin, Bovine; D016718:Arachidonic Acid; D013739:Testosterone; C025086:15-hydroperoxy-5,8,11,13-eicosatetraenoic acid; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid; D008084:Lipoxygenase