Interferon gamma and lipopolysaccharide promote macrophage adherence to basement membrane glycoproteins.
The ability of thioglycollate (TG)-elicited peritoneal macrophages, a population of recently recruited monocytes, to adhere to the basement membrane glycoproteins laminin and type IV collagen is not a constitutive function of these cells. Adherence can be induced, however, by treatment with IFN-gamma and LPS. In general, IFN-gamma is more potent than LPS in promoting this adherence. Maximal adherence, however, is observed when IFN-gamma (greater than or equal to 5 U/ml) is used together with LPS (2.0 ng/ml). These requirements parallel the conditions needed to obtain tumoricidal activation of TG-elicited macrophages. Adherence to laminin, in the presence of these stimuli, is transient, being maximal at 8 h after their addition and diminishing with longer periods of incubation. In contrast, adherence to type IV collagen does not appear to be transient and IFN-gamma and LPS induce a more prolonged association of macrophages with this substratum.
Shaw
L M
LM
Laboratory of Cancer Biology, New England Deaconess Hospital, Harvard Medical School, Boston, Massachusetts 02115.
Mercurio
A M
AM
D000818:Animals; D001485:Basement Membrane / Q000502:physiology*; D002448:Cell Adhesion; D003094:Collagen / Q000502:physiology; D005353:Fibronectins / Q000502:physiology; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000494:pharmacology*; D007797:Laminin / Q000502:physiology; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008262:Macrophage Activation; D008264:Macrophages / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D013864:Thioglycolates; D013997:Time Factors
D005353:Fibronectins; D007797:Laminin; D008070:Lipopolysaccharides; D013864:Thioglycolates; D007371:Interferon-gamma; D003094:Collagen