title: Strain differences in the opsonisation of Staphylococcus epidermidis.
abstract: Ten isolates of coagulase-negative staphylococci, collected from patients receiving treatment with continuous ambulatory peritoneal dialysis (CAPD), exhibited marked differences in the degree of opsonisation when incubated in 10% and 1% pooled human serum, 10% and 1% heat-treated serum, Hanks' Balanced Salt Solution, and timed peritoneal dialysis (PD) effluent. The addition of exogenous IgG to PD effluent results in a greater increase in opsonisation in those fluids with the weakest inherent opsonic activity, but is ineffective against the majority of isolates in the absence of heat-labile opsonic activity. The results of this in vitro study suggest that host resistance to CAPD peritonitis due to coagulase-negative staphylococci may be determined as much by the characteristics of the contaminating strain, as by the opsonising activity of PD effluent.
authors.affiliation: Department of Renal Medicine, Guy's Hospital, London Bridge, UK.
authors.forename: D N
authors.initials: DN
authors.lastname: Bennett-Jones
authors.forename: V M
authors.initials: VM
authors.lastname: Yewdall
authors.forename: C M
authors.initials: CM
authors.lastname: Gillespie
authors.forename: C S
authors.initials: CS
authors.lastname: Ogg
authors.forename: J S
authors.initials: JS
authors.lastname: Cameron
mesh_terms: D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D008163:Luminescent Measurements; D009504:Neutrophils / Q000276:immunology*; D009895:Opsonin Proteins / Q000276:immunology*; D010531:Peritoneal Dialysis, Continuous Ambulatory / Q000009:adverse effects; D010538:Peritonitis / Q000209:etiology / Q000276:immunology; D010587:Phagocytosis / Q000276:immunology*; D013203:Staphylococcal Infections / Q000276:immunology / Q000382:microbiology; D013212:Staphylococcus epidermidis / Q000276:immunology*
chemical_list: D007074:Immunoglobulin G; D009895:Opsonin Proteins