title: Gamete processing in an environmental chamber: effect on in vitro fertilization outcome.
abstract: A study of mouse gamete processing for in vitro fertilization (IVF) under various conditions showed that it is necessary to control the atmosphere if the temperature is raised from 22 degrees C to 37 degrees C. The data suggest that maximum IVF success is attained by processing the gametes at 37 degrees C, under an atmosphere of 5% O2 and 5% CO2, and overlaying the medium with silicone oil.
authors.affiliation: Research Institute and Department of Gynecology, Cleveland Clinic Foundation, Ohio 44195-5240.
authors.forename: R B
authors.initials: RB
authors.lastname: Gwatkin
authors.forename: M M
authors.initials: MM
authors.lastname: Quigley
authors.forename: R L
authors.initials: RL
authors.lastname: Collins
mesh_terms: D000818:Animals; D001273:Atmosphere Exposure Chambers; D002245:Carbon Dioxide / Q000502:physiology; D005314:Embryonic and Fetal Development; D005260:Female; D005307:Fertilization in Vitro; D006813:Humidity; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008954:Models, Biological; D009865:Oocytes / Q000502:physiology*; D010100:Oxygen / Q000502:physiology; D010313:Partial Pressure; D013094:Spermatozoa / Q000502:physiology*; D013696:Temperature
chemical_list: D002245:Carbon Dioxide; D010100:Oxygen