Identification of the inhibitor of the plasminogen activator as the major protein secreted by endothelial rat liver cells.
Freshly isolated Kupffer and endothelial liver cells exhibit a rate of 'de novo' protein synthesis which is twice as high per mg cell protein as that of parenchymal liver cells and contribute significantly (7.5% and 5.9%, respectively) to total liver protein secretion. In parenchymal cells the main secretory protein is a 68 kDa protein (containing 19% fo the secreted radioactivity, presumably albumin). In Kupffer cells a 49 kDa protein contains 8% of the secreted radioactivity, while in endothelial liver cells a 55 kDa protein is the most prominent secretory protein (containing 11% of the secreted radioactivity). By aid of a specific antibody the 55 kDa protein was identified as the inhibitor of the plasminogen activator and in the liver this protein was only secreted by the endothelial cells.
Kuiper
J
J
Center for Bio-Pharmaceutical Sciences, University of Leiden, Sylvius Laboratories, The Netherlands.
Kamps
J A
JA
Van Berkel
T J
TJ
D000818:Animals; D003078:Colchicine / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D004727:Endothelium / Q000378:metabolism; D007163:Immunosorbent Techniques; D007728:Kupffer Cells / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008970:Molecular Weight; D010960:Plasminogen Activators / Q000037:antagonists & inhibitors*; D015849:Plasminogen Inactivators; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D014633:Valine / Q000378:metabolism
D015849:Plasminogen Inactivators; D011506:Proteins; D003513:Cycloheximide; D010960:Plasminogen Activators; D014633:Valine; D003078:Colchicine