title: Caffeine as main interfering compound in radioimmunoassay of aflatoxin B1 in coffee samples.
abstract: The content of caffeine in coffee extracts prepared for radioimmunoassay of aflatoxin B1 was determined by gas chromatography. The extracts from coffee beans and decaffeinated coffee contained 1.76-4.60 and 0.71-0.85 g caffeine/kg, respectively. These concentrations of caffeine caused false results in radioimmunoassay of aflatoxin B1 in the range 1.0-2.8 micrograms/kg for coffee beans and 0.3-0.4 micrograms/kg for decaffeinated coffee.
authors.affiliation: Institute of Chemical Technology, Prague, Czechoslovakia.
authors.forename: P
authors.initials: P
authors.lastname: Rauch
authors.forename: I
authors.initials: I
authors.lastname: Víden
authors.forename: T
authors.initials: T
authors.lastname: Davídek
authors.forename: J
authors.initials: J
authors.lastname: Velísek
authors.forename: L
authors.initials: L
authors.lastname: Fukal
mesh_terms: D016604:Aflatoxin B1; D000348:Aflatoxins / Q000032:analysis*; D002110:Caffeine / Q000032:analysis*; D002855:Chromatography, Thin Layer; D003069:Coffee / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D011863:Radioimmunoassay; D012997:Solvents
chemical_list: D000348:Aflatoxins; D003069:Coffee; D012997:Solvents; D002110:Caffeine; D016604:Aflatoxin B1