Transformation of human kidney epithelial cells to tumorigenicity by nickel(II) and V-HA-RAS oncogene.
Nickel is a toxic metal of environmental concern that has been found to be carcinogenic in man and animals. Primary human kidney (NHKE) cells were immortalized or rescued from senescence after exposure to NiSO4. The cell lines (IHKE) displayed abnormal karyotype and anchorage independent growth was observed. However, none of the IHKE cells produced tumor upon injection into athymic nude mice. Transfer of the v-Ha-ras oncogene into IHKE cells induced conversion of the immortalized cells into cell lines (THKE) that were tumorigenic when transplanted into athymic nude mice. Ha-ras DNA was present in the transformed cell lines and expressed at high level.
Haugen
A
A
Department of Toxicology, National Institute of Occupational Health, Oslo, Norway.
Ryberg
D
D
Hansteen
I L
IL
Dalen
H
H
D000818:Animals; D002273:Carcinogens; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D002875:Chromosomes / Q000187:drug effects / Q000648:ultrastructure; D002999:Clone Cells / Q000187:drug effects; D004273:DNA, Neoplasm / Q000187:drug effects; D004847:Epithelial Cells; D011905:Genes, ras / Q000187:drug effects*; D006801:Humans; D007668:Kidney / Q000166:cytology*; D051379:Mice; D008819:Mice, Nude; D009532:Nickel / Q000633:toxicity*; D012334:RNA, Neoplasm / Q000187:drug effects; D011995:Recombination, Genetic
D002273:Carcinogens; D004273:DNA, Neoplasm; D012334:RNA, Neoplasm; D009532:Nickel