Transformation of a filamentous cyanobacterium by electroporation.
The filamentous cyanobacterium Anabaena sp. strain M131 was transformed with the shuttle vector pRL6 by electroporation. Optimum conditions for electroporation required relatively high field strengths with short time constants. Restriction significantly lowered the efficiency of transformation. A plasmid containing a single unmodified AvaII restriction site transformed cells with about 100-fold-lower efficiency than did the same plasmid with a modified restriction site.
Thiel
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Department of Biology, University of Missouri, St. Louis 63121.
Poo
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H
D002463:Cell Membrane Permeability; D000458:Cyanobacteria / Q000235:genetics*; D004560:Electricity; D010957:Plasmids; D014170:Transformation, Genetic