Microassay for N-acetyltransferase activity using high-performance liquid chromatography with electrochemical detection.
A rapid, sensitive procedure has been developed for determination of N-acetyltransferase activity against octopamine, dopamine and 5-hydroxytryptamine. The assay, which is performed in a volume of 10 microliters, is based upon the separation and detection of monoamine substrates and their N-acetylated derivatives using high-performance liquid chromatography with electrochemical detection. The method has been used to measure N-acetyltransferase activity against octopamine, dopamine and 5-hydroxytryptamine in the cerebral ganglion of the American cockroach, Periplaneta americana and to study bi-substrate kinetics of the enzyme.
Martin
R J
RJ
Department of Biology, University of Waterloo, Ontario, Canada.
Downer
R G
RG
D006916:5-Hydroxytryptophan / Q000378:metabolism; D000123:Acetyltransferases / Q000378:metabolism*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003058:Cockroaches; D004298:Dopamine / Q000378:metabolism; D004563:Electrochemistry; D008297:Male; D009655:Octopamine / Q000378:metabolism; D013696:Temperature
D009655:Octopamine; D006916:5-Hydroxytryptophan; D000123:Acetyltransferases; D004298:Dopamine