Regulation of LacZ mRNA translatability in a cell-free system at heat shock by the last four sense codons.
Under heat shock conditions translation of Xenopus laevis normal mRNAs in a rabbit reticulocyte cell-free system is blocked whereas hsp70 mRNA is translated. mRNA for E. coli beta-galactosidase containing the last four sense codons of Drosophila hsp70 at its 3'-end was constructed. This mRNA is efficiently translated in a rabbit reticulocyte cell-free system at 43 degrees C.
Denisenko
O N
ON
Institute of Protein Research, Academy of Sciences of the USSR, Pushchino, Moscow region.
Yarchuk
O B
OB
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002474:Cell-Free System; D003062:Codon; D003593:Cytoplasm / Q000378:metabolism; D004330:Drosophila / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D005696:Galactosidases / Q000235:genetics*; D005786:Gene Expression Regulation; D006360:Heat-Shock Proteins / Q000235:genetics*; D006358:Hot Temperature; D008969:Molecular Sequence Data; D009994:Osmolar Concentration; D010957:Plasmids; D011188:Potassium / Q000494:pharmacology; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D014158:Transcription, Genetic; D014982:Xenopus laevis; D001616:beta-Galactosidase / Q000235:genetics*
D003062:Codon; D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D005696:Galactosidases; D001616:beta-Galactosidase; D011188:Potassium