Effect of chemical modification of histidine and tyrosine residues in toxic shock syndrome toxin 1 on the serologic and mitogenic activities of the toxin.
Modification of three or four of the five histidine residues in the toxic shock syndrome toxin 1 (TSST-1) with diethylpyrocarbonate did not inhibit the precipitin reaction of the modified TSST-1 with polyvalent antisera to the toxin. Monoclonal antibody 7T did not react with the modified TSST-1, but monoclonal antibody 8T did react with the toxin. Up to 50% of the mitogenic reaction of TSST-1 was inhibited by the histidine modification. Modification of one or two of the nine tyrosine residues in TSST-1 did not inhibit the precipitin reaction with polyclonal antisera to the toxin but did inhibit 85% of the mitogenic reaction.
Kokan-Moore
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NP
Department of Food Microbiology and Toxicology, University of Wisconsin-Madison 53706.
Bergdoll
M S
MS
D000818:Animals; D000937:Antigen-Antibody Reactions; D001427:Bacterial Toxins / Q000276:immunology* / Q000494:pharmacology; D004047:Diethyl Pyrocarbonate; D004768:Enterotoxins / Q000276:immunology* / Q000494:pharmacology; D006639:Histidine / Q000378:metabolism*; D005779:Immunodiffusion; D008213:Lymphocyte Activation; D051379:Mice; D008934:Mitogens / Q000494:pharmacology*; D011487:Protein Conformation; D013211:Staphylococcus aureus / Q000276:immunology*; D018089:Superantigens; D014443:Tyrosine / Q000378:metabolism*
D001427:Bacterial Toxins; D004768:Enterotoxins; D008934:Mitogens; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal; D014443:Tyrosine; D006639:Histidine; D004047:Diethyl Pyrocarbonate