Choline and betaine as inducer agents of Pseudomonas aeruginosa phospholipase C activity in high phosphate medium.
In Pseudomonas aeruginosa, choline or betaine employed as the sole carbon and nitrogen source in a high phosphate medium induced a phospholipase C and an acid phosphatase activity but not an alkaline phosphatase activity. The P. aeruginosa strain utilized in this work does not possess a constitutive phospholipase C, since under culture conditions identical to those utilized by other authors (J. Bacteriol. 93, 670-674 (1967) and J. Bacteriol. 150, 730-738 (1982), our phospholipase C proved to be an inorganic phosphate-repressible enzyme. These findings enable us to conclude that although the phosphate control for the synthesis of phospholipase C may exist, it is expressed only under certain favorable culture conditions.
Lucchesi
G I
GI
Departamento de Biologia Molecular, Facultad de Ciencias Exactas, Universidad Nacional de Rio Cuarto, Cordoba, Argentina.
Lisa
T A
TA
Domenech
C E
CE
D000135:Acid Phosphatase / Q000096:biosynthesis; D000469:Alkaline Phosphatase / Q000096:biosynthesis; D001622:Betaine / Q000494:pharmacology; D002794:Choline / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D010710:Phosphates / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology*; D010738:Type C Phospholipases / Q000096:biosynthesis*
D010710:Phosphates; D001622:Betaine; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D010738:Type C Phospholipases; D002794:Choline