title: The Drosophila melanogaster RPS17 gene encoding ribosomal protein S17.
abstract: A human ribosomal protein S17 cDNA [Chen et al., Proc. Natl. Acad. Sci. USA 83 (1986) 6907-6911] was used as heterologous probe to isolate S17 clones from Drosophila genomic and cDNA recombinant libraries. Five S17 genomic clones were recognized; all contained overlapping regions of a single chromosomal site. Subsequently the Drosophila RPS17 gene was mapped by in situ hybridization to chromosome 3L, band 67B1-5. The locus spans approximately 1000 bp of DNA and includes four exons. It is preceded by conventional CAAT and TATA RNA polymerase II promoter motifs. The 131 amino acid protein encoded within Drosophila RPS17 is similar to ribosomal proteins from several other eukaryotes. Comparison of eukaryotic S17 proteins' primary structures as well as the number and location of their genes' intervening sequences suggest that S17 is a relatively recent addition to the ribosomal protein family, probably post-dating divergence of eukaryotes and prokaryotes.
authors.affiliation: Center for Basic Cancer Research, Division of Biology, Kansas State University, Manhattan 66506.
authors.forename: C
authors.initials: C
authors.lastname: Maki
authors.forename: D D
authors.initials: DD
authors.lastname: Rhoads
authors.forename: M J
authors.initials: MJ
authors.lastname: Stewart
authors.forename: B
authors.initials: B
authors.lastname: Van Slyke
authors.forename: D J
authors.initials: DJ
authors.lastname: Roufa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D004247:DNA / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics; D005091:Exons; D006801:Humans; D015151:Immunoblotting; D007438:Introns; D008969:Molecular Sequence Data; D005810:Multigene Family; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D015183:Restriction Mapping; D012269:Ribosomal Proteins / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D012333:RNA, Messenger; D012269:Ribosomal Proteins; C037084:ribosomal protein S17; D004247:DNA