title: Nonenzymatic glycation of human blood platelet proteins.
abstract: We studied 11 diabetic patients, all of whom had severe atherothrombotic disease, and 11 normal controls. Overall glycation was assessed by the extent of incorporation of [3H]-NaBH4 into fructosyl lysine separated from whole platelet proteins following aminoacid analysis. Fructosyl lysine represented 5.7% +/- 1.0 S.D. of the total radioactivity in the normal whole platelet samples. Increased glycation was observed in platelets from 5 of the 11 diabetics. Platelet glycation did not correlate with glycation of hemoglobin or albumin. The pattern of glycation of various platelet proteins in whole platelets, as determined by the incorporation of [3H]-NaBH4 into electrophoretically separated proteins did not display selectivity, although myosin and glycoproteins IIb and IIIa showed relatively increased levels of [3H]-NaBH4 incorporation. Artificially glycated platelet membranes exhibited glycation mainly in proteins corresponding to the electrophoretic mobility of myosin, glycoproteins IIb and IIIa.
authors.affiliation: Atherosclerosis Program, Rehabilitation Institute of Chicago, Illinois.
authors.forename: I
authors.initials: I
authors.lastname: Cohen
authors.forename: D
authors.initials: D
authors.lastname: Burk
authors.forename: R J
authors.initials: RJ
authors.lastname: Fullerton
authors.forename: A
authors.initials: A
authors.lastname: Veis
authors.forename: D
authors.initials: D
authors.lastname: Green
mesh_terms: D000596:Amino Acids / Q000032:analysis; D001792:Blood Platelets / Q000378:metabolism*; D001798:Blood Proteins / Q000378:metabolism*; D001894:Borohydrides; D003920:Diabetes Mellitus / Q000097:blood*; D006442:Glycated Hemoglobin / Q000378:metabolism; D017127:Glycation End Products, Advanced; D006031:Glycosylation; D006801:Humans; D006868:Hydrolysis; D008239:Lysine / Q000031:analogs & derivatives / Q000097:blood; D012709:Serum Albumin / Q000378:metabolism; D000093423:Glycated Serum Albumin
chemical_list: D000596:Amino Acids; D001798:Blood Proteins; D001894:Borohydrides; D006442:Glycated Hemoglobin; D017127:Glycation End Products, Advanced; D008239:Lysine; D012709:Serum Albumin; D000093423:Glycated Serum Albumin; C033186:fructosyl-lysine; C025364:sodium borohydride