title: A protein concentrate from distillery yeast, and its application to supplement corn tortillas.
abstract: The purpose of this study was to increase the protein quality of tortillas supplementing them with a protein concentrate obtained from the yeast (Saccharomyces cerevisiae) cream from an alcohol distillery. Rupture of the yeast cell wall was studied, and a procedure for obtaining a high percentage of broken cells from the yeast was developed. A protein concentrate was obtained from the cell-wall free portion. Techniques were developed to obtain the protein concentrate with a low level of nucleic acids. The latter were reduced 91%, and the protein increased 55% with respect to the initial yeast. Different levels of protein concentrate were added to dough made from corn of the Nutricta variety. The resulting tortillas were submitted to a taste panel for sensory evaluation, and the results were statistically analyzed. The highest level of supplementation that did not affect negatively acceptation by the panel was 18% dry weight. In the tortillas with the highest acceptable level of supplementation, protein content increased 60% when compared to the control tortillas, and a significant improvement in lysine content was observed. Nucleic acid content was low, having been reduced to levels which did not represent a limiting factor for the use of yeast in a product destined for human consumption.
authors.affiliation: Central American Institute for Research in Industry (ICAITI), Guatemala City, Guatemala.
authors.forename: M C
authors.initials: MC
authors.lastname: de Arriola
authors.forename: M
authors.initials: M
authors.lastname: de Zepeda
authors.forename: C
authors.initials: C
authors.lastname: Rolz
mesh_terms: D000601:Amino Acids, Essential / Q000032:analysis*; D004044:Dietary Proteins / Q000032:analysis*; D005511:Food Handling; D005524:Food Technology; D005527:Food, Fortified; D006166:Guatemala; D009696:Nucleic Acids / Q000032:analysis*; D012441:Saccharomyces cerevisiae / Q000032:analysis*; D003313:Zea mays
chemical_list: D000601:Amino Acids, Essential; D004044:Dietary Proteins; D009696:Nucleic Acids