Nonenzymatic isolation of Trichinella pseudospiralis infective L1 larvae at pH 7.4.
Over half of the number of Trichinella pseudospiralis infective L1 larvae recovered from host carcasses by pepsin-HCl digestion were isolated from homogenized carcasses incubated in HBSS. More worms isolated by the latter method were viable compared to those isolated by pepsin-HCl digestion. When host carcasses infected with T. pseudospiralis were diced into pieces and incubated in HBSS, 30% more worms were recovered than from homogenized carcasses incubated in HBSS as above, and the majority of worms acquired by the former method were viable. The infectivity of T. pseudospiralis infective L1 larvae isolated from homogenized muscle in HBSS was 3.9 times greater than that for larvae recovered from homogenized carcasses by pepsin-HCl digestion. Only 4% and 0.8% of the number of T. spiralis recovered from homogenized muscle by pepsin-HCl digestion were isolated from homogenized or diced muscle incubated in HBSS, respectively. Fewer T. spiralis isolated from homogenized tissue in HBSS were viable compared to those recovered from homogenized carcasses digested in pepsin-HCl or diced carcasses incubated in HBSS.
Stewart
G L
GL
Department of Biology, University of Texas, Arlington 76019.
Deford
J
J
D000818:Animals; D006851:Hydrochloric Acid / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007814:Larva / Q000302:isolation & purification; D008297:Male; D008722:Methods; D051379:Mice; D008813:Mice, Inbred ICR; D009132:Muscles / Q000469:parasitology; D010434:Pepsin A / Q000494:pharmacology; D012965:Sodium Chloride; D014234:Trichinella / Q000302:isolation & purification* / Q000472:pathogenicity
D012965:Sodium Chloride; D010434:Pepsin A; D006851:Hydrochloric Acid