title: Dot-enzyme immunoassay for visual detection of Brucella melitensis antibodies.
abstract: An indirect enzyme-linked immunosorbent assay, using antigen coupled to paper, has been adapted for the detection of Brucella melitensis antibodies. Optimum conditions were achieved by incubation of 1 ml of diluted serum with a single piece of paper coated with purified Brucella antigens for a period of one hour, and by addition of a goat anti-human enzyme conjugate antibody for one hour again. Under these conditions 80 human sera were examined and the results obtained were compared with Wright agglutination test.
authors.affiliation: Institute of Microbiology, Faculty of Medicine and Surgery, University of Messina, Italy.
authors.forename: M S
authors.initials: MS
authors.lastname: Leonardi
authors.forename: S
authors.initials: S
authors.lastname: Zummo
authors.forename: D
authors.initials: D
authors.lastname: Gazzara
mesh_terms: D000372:Agglutination Tests; D000907:Antibodies, Bacterial / Q000032:analysis*; D002002:Brucella / Q000276:immunology*; D002006:Brucellosis / Q000175:diagnosis* / Q000382:microbiology; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male
chemical_list: D000907:Antibodies, Bacterial