Single channel recordings from basolateral membranes of frog urinary bladders.
A methodology is described to obtain, in frog urinary bladder, isolated epithelial layers suitable for patch clamp studies. This was achieved combining negative pressure, enzymatic digestion, and microdissection. Single channels recordings were obtained from the basolateral membranes while the tissue maintained its general structure and polarity.
Parisi
M
M
Departamento de Fisiología, Facultad de Medicina, Universidad de Buenos Aires.
Uchitel
O
O
D000818:Animals; D004848:Epithelium / Q000502:physiology; D006652:Histological Techniques; D007473:Ion Channels / Q000502:physiology*; D008564:Membrane Potentials / Q000187:drug effects; D011893:Rana esculenta; D001743:Urinary Bladder / Q000502:physiology*; D014667:Vasopressins / Q000494:pharmacology
D007473:Ion Channels; D014667:Vasopressins