The concentration of apolipoprotein A-II in human peripheral lymph.
The concentration of apolipoprotein A-I (apoA-I) and of apolipoprotein A-II (apoA-II) was determined immunoelectrophoretically in lymph and plasma of six subjects. The concentration in lymph of apoA-I was 20.3 +/- 3.1 mg/dl, that of apoA-II was 4.6 +/- 0.5 mg/dl. The ratio of the concentration in lymph over that in plasma (CL/CP) for apoA-I was 0.14 +/- 0.1, that for apoA-II 0.14 +/- 0.01. Lymph and plasma samples from two subjects were fractionated by exclusion chromatography and the concentration of both apolipoproteins in resulting fractions was determined immunoelectrophoretically. ApoA-I of lymph eluted with fractions spanning a wider range of particle sizes than plasma apoA-I, while lymph apoA-II eluted predominantly with fractions that contained particles corresponding in size to plasma apoA-II-containing particles. It appears that the largest and smallest lymph HDL represent subspecies of Lp(A-I without A-II). These findings are discussed in the context of their possible bearing on initial stages of reverse transport of cholesterol.
Reichl
D
D
Medical Professorial Unit, St. Bartholomew's Hospital, London, U.K.
Rudra
D N
DN
Pflug
J
J
D016632:Apolipoprotein A-I; D016633:Apolipoprotein A-II; D001054:Apolipoproteins A / Q000032:analysis* / Q000097:blood; D006801:Humans; D007122:Immunoelectrophoresis; D008075:Lipoproteins, HDL / Q000097:blood; D008196:Lymph / Q000032:analysis*; D012016:Reference Values
D016632:Apolipoprotein A-I; D016633:Apolipoprotein A-II; D001054:Apolipoproteins A; D008075:Lipoproteins, HDL