DNA-DNA homology among lactose- and sucrose-fermenting transconjugants from Lactococcus lactis strains exhibiting reduced bacteriophage sensitivity.
DNA-DNA homology between a reduced bacteriophage sensitivity (Rbs+) probe and DNA from both Rbs+ and Rbs- Lactococcus lactis strains was examined. Homology was detected between the probe and five plasmids (pCI750, pCC34, pEB56, pNP2, and pJS88) isolated from lactose-positive Rbs+ transconjugants and between the probe and genomic DNA of a sucrose-positive Rbs+ transconjugant. Additionally, hybridizations conducted between the probe and plasmids reported to encode abortive bacteriophage infection indicated homology with pTR2030 but not with pBF61 and pGBK17. The results suggest that a common genetic determinant(s) may be present in a variety of lactococcal plasmids coding for Rbs+.
Steele
J L
JL
Department of Genetics and Cell Biology, University of Minnesota, St. Paul 55108.
Murphy
M C
MC
Daly
C
C
McKay
L L
LL
D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000235:genetics; D005285:Fermentation; D013294:Lactococcus lactis / Q000235:genetics* / Q000378:metabolism; D007785:Lactose / Q000378:metabolism*; D010641:Phenotype; D012689:Sequence Homology, Nucleic Acid; D013395:Sucrose / Q000378:metabolism*
D004269:DNA, Bacterial; D013395:Sucrose; D007785:Lactose