Glycosylation characteristics of the mouse liver lactogenic receptor.
The structural characteristics and glycosylation properties of the lactogenic receptor were examined in 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonate (CHAPS)-solubilized plasma membranes from female mouse liver. The specific binding of the radioiodinated human growth hormone [( 125I]hGH) was displaced with an equivalent potency by both hGH and prolactin. After a mild neuraminidase treatment, this binding was increased by 40%, as a result of an increase in receptor affinity. Affinity chromatography on immobilized lectins revealed that the [125I]hGH-receptor complexes were specifically retained and eluted from ricin lectin-agarose, concanavalin A and lentil lectin, indicating the presence of N-linked glycans. Covalent cross-linking of solubilized [125I]hGH-receptor complexes with disuccinimidyl suberate, followed by analysis by sodium dodecyl sulfate-gel electrophoresis (SDS-PAGE) under reducing conditions, and autoradiography resulted in the appearance of two bands with apparent Mr approximately 62,000 and approximately 100,000. The labelling of these bands was prevented by unlabelled hGH or ovine prolactin (oPrl) but not by bovine growth hormone (bGH). Neuraminidase treatment of the two receptor forms resulted in increased electrophoretic mobility which was inhibited by simultaneous addition of sialyl-lactose, a neuraminidase substrate. The both cross-linked forms were unaffected by endoglycosidase H, while endoglycosidase F decreased the molecular weight of each of the forms by about 8000 Da, yielding bands at Mr approximately 54,000 and approximately 92,000. In conclusion, taking into account that hGH is a Mr 22,000 polypeptide, the two forms of the receptor correspond to glycoproteins of Mr approximately 40,000 and approximately 78,000, respectively. They contain polypeptide backbones of Mr approximately 32,000 and approximately 70,000, and complex N-linked oligosaccharide chains with terminal sialic acid residues which could be involved in receptor binding affinity.
Lascols
O
O
Laboratoire de Biochimie, I.N.S.E.R.M. U181, Faculté de Médecine Saint-Antoine, Paris, France.
Capeau
J
J
Cherqui
G
G
Caron
M
M
Bachimont
J
J
Picard
J
J
D000118:Acetylglucosaminidase; D000818:Animals; D002793:Cholic Acids; D002846:Chromatography, Affinity; D003432:Cross-Linking Reagents; D003902:Detergents; D005260:Female; D006026:Glycoside Hydrolases; D006031:Glycosylation; D037102:Lectins; D008099:Liver / Q000378:metabolism*; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D008970:Molecular Weight; D009439:Neuraminidase; D011967:Receptors, Gonadotropin / Q000302:isolation & purification / Q000378:metabolism*; D011981:Receptors, Prolactin / Q000378:metabolism*; D012794:Sialic Acids / Q000378:metabolism; D013388:Succinimides
D002793:Cholic Acids; D003432:Cross-Linking Reagents; D003902:Detergents; D037102:Lectins; D008562:Membrane Glycoproteins; D011967:Receptors, Gonadotropin; D011981:Receptors, Prolactin; D012794:Sialic Acids; D013388:Succinimides; D006026:Glycoside Hydrolases; D009439:Neuraminidase; D000118:Acetylglucosaminidase; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate; C019358:disuccinimidyl suberate