Quantitation of the beta-bungarotoxin-induced release of lactate dehydrogenase from cerebrocortical synaptosomes.
Treatment with beta-bungarotoxin for 1 h induces lactate dehydrogenase release from cerebrocortical synaptosomes. The effect is Ca2+-dependent as suggested by the inhibition observed with EGTA. An inhibition of the effect can be also obtained by addition of Sr2+ ions, suggesting that the phospholipase A2 activity associated to the toxin is involved in the efflux of the enzyme. The beta-bungarotoxin-induced release of synaptoplasmic lactate dehydrogenase is a saturable effect, showing a half-maximal effect concentration of 32 nM and a maximal efflux of 25% of the total synaptosomal enzyme as calculated by double-reciprocal plot.
Martinez
C
C
Departamento de Bioquímica, Biología Molecular y Celular, Universidad de Zaragoza, Facultad de Veterinaria, Spain.
Pérez-Martos
A
A
López Pérez
M J
MJ
D000818:Animals; D002038:Bungarotoxins / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology; D002540:Cerebral Cortex / Q000187:drug effects / Q000201:enzymology*; D004533:Egtazic Acid / Q000494:pharmacology; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D012756:Sheep; D013324:Strontium / Q000494:pharmacology; D013574:Synaptosomes / Q000187:drug effects / Q000201:enzymology*
D002038:Bungarotoxins; D004533:Egtazic Acid; D007770:L-Lactate Dehydrogenase; D010741:Phospholipases A; D054467:Phospholipases A2; D002118:Calcium; D013324:Strontium