[Basic study for measurement of radiation damage of DNA using a flow cytometry].
Radiation damage of DNA in HeLa cells was measured according to a method reported by Milner, et al. Cells were suspended in lysis buffer to obtain nucleoid. They were stained with ethidium bromide immediately before the measurement by using a system of flow cytometry. The mean position of channels for forward scatters increased at first and decreased thereafter as the concentration of ethidium bromide increased. The biphasic response disappeared with irradiation given to the cells. When the concentration of ethidium bromide was constant, the mean position of channels for forward scatters increased as the dose of irradiation increased. It might be possible to use the method in predicting the response of a tumor to irradiation in the clinical practice.
Akine
Y
Y
Department of Radiation Therapy, National Cancer Center Hospital, Tokyo, Japan.
Nomura
K
K
Iwasaki
K
K
D002467:Cell Nucleus; D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage; D004996:Ethidium; D005434:Flow Cytometry / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D011829:Radiation Dosage
D004247:DNA; D004996:Ethidium