Molecular isolation and analysis of the erect wing locus in Drosophila melanogaster.
The molecular study of the erect wing (ewg) locus was initiated by isolating DNA in the 1A8-1B1 interval of the X chromosome. Previous developmental genetic analyses of the mutant alleles at the ewg locus have demonstrated that the wild-type ewg product is essential during embryogenesis and is required postembryonically at least for the development of the indirect flight muscle. To define the ewg-encoding DNA, chromosomal breakpoints that genetically flank the ewg locus were used. P-element-mediated transformation followed by subsequent rescue of the ewg-lethal alleles has defined a 11.5-kilobase genomic fragment as encoding the ewg locus. Northern blot analysis of transcription from this DNA has revealed a complex pattern of transcription with respect to both size and developmental profile. Tissue distribution of putative ewg transcription was examined by in situ hybridization to 6- to 14-h-old embryonic sections. These sections revealed that the expression of putative ewg messages is limited to the central nervous system-derived structures and not observed within the mesoderm during this developmental stage.
Fleming
R J
RJ
Department of Biology, Brandeis University, Waltham, Massachusetts 02254.
DeSimone
S M
SM
White
K
K
D000818:Animals; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D009154:Mutation; D010641:Phenotype; D015347:RNA Probes; D014018:Tissue Distribution; D014158:Transcription, Genetic; D014921:Wings, Animal / Q000002:abnormalities
D015347:RNA Probes; D004247:DNA