title: Recent developments in the molecular genetics and biology of self-incompatibility.
abstract: A summary of recent work on molecular aspects of self-incompatibility in Nicotiana alata is presented. The amino acid sequences of style proteins corresponding to different S-alleles of N. alata have a high level of homology in some regions and are variable in other regions. The regions of homology include N-terminal sequences as well as most of the glycosylation sites and cysteine residues. The glycosyl substituents may consist of a number of 'glycoforms'. The isolated style S-glycoproteins inhibit in vitro growth of pollen tubes. The S-glycoproteins tested inhibited the growth of pollen of several S-genotypes, and there was some specificity in the interaction. Heat treatment of the isolated S-glycoproteins dramatically increased their activity as inhibitors of pollen tube growth, although the specificity in the interaction was lost. The nature of the S-allele products in pollen is not yet established.
authors.affiliation: Plant Cell Biology Research Centre, School of Botany, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: A E
authors.initials: AE
authors.lastname: Clarke
authors.forename: M A
authors.initials: MA
authors.lastname: Anderson
authors.forename: A
authors.initials: A
authors.lastname: Atkinson
authors.forename: A
authors.initials: A
authors.lastname: Bacic
authors.forename: P R
authors.initials: PR
authors.lastname: Ebert
authors.forename: W
authors.initials: W
authors.lastname: Jahnen
authors.forename: W M
authors.initials: WM
authors.lastname: Lush
authors.forename: S L
authors.initials: SL
authors.lastname: Mau
authors.forename: J R
authors.initials: JR
authors.lastname: Woodward
mesh_terms: D000483:Alleles; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D006023:Glycoproteins / Q000235:genetics; D008967:Molecular Biology; D010940:Plant Proteins / Q000235:genetics; D010944:Plants / Q000235:genetics*; D010947:Plants, Toxic; D012098:Reproduction / Q000235:genetics; D014026:Nicotiana / Q000235:genetics
chemical_list: D006023:Glycoproteins; D010940:Plant Proteins; D004247:DNA