[Properties of factor VIII/von Willebrand factor in two highly-purified factor VIII concentrates, Hemofil M and Monoclate, using monoclonal antibodies].
Properties of F. VIII/vWF in highly-purified factor VIII concentrates were examined using monoclonal antibodies. The F. VIII: C levels obtained with the chromogenic assay agreed with those obtained with the one-stage clotting method. The ratio between F. VIII: Ag and F. VII: C in Hemofil M and Monoclate was 1.09 and 1.34, respectively. The F. VIII: Ag levels assayed by monoclonal ELISAs were the same as those assayed by polyclonal ELISA, except that those assayed by C 5-ELISA tended to be higher. The ratio between F. VIII: Ag and vWF: Ag in Hemofil M and Monoclate was 105 and 45, respectively. Both concentrates lacked the large multimers of vWF and showed the intensification of the satellite bands. SDS-PAGE patterns showed almost no contamination. Immunoblot analysis revealed that F. VIII in both concentrates could react with 6 kinds of monoclonal antibodies to F. VIII. These results suggest that the fundamental structure of F. VIII molecule for coagulant activity in both concentrates are preserved.
Tanaka
I
I
Yoshioka
A
A
Shima
M
M
Fujiwara
T
T
Terada
S
S
Nakai
H
H
Miyata
S
S
Sawamoto
Y
Y
Kamisue
S
S
Fukui
H
H
D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis; D001780:Blood Coagulation Tests; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005169:Factor VIII / Q000032:analysis* / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D014841:von Willebrand Factor / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000941:Antigens; D014841:von Willebrand Factor; D005169:Factor VIII