title: Comparison of different flow injection approaches to the automatic determination of enzymatic activity.
abstract: Three configurations based on the principles behind flow injection analysis (FIA) are proposed for the automatic determination of enzymatic activity. The proposed approaches are normal, stopped-flow and open-closed FIA. The comparative study of the methods developed from these approaches allows the establishment of the scope of the application of each, with inherent advantages and disadvantages.
authors.affiliation: Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Fernandez-Romero
authors.forename: M D
authors.initials: MD
authors.lastname: Luque de Castro
authors.forename: M
authors.initials: M
authors.lastname: Valcarcel
mesh_terms: D004798:Enzymes / Q000032:analysis / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007770:L-Lactate Dehydrogenase / Q000032:analysis / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D009243:NAD / Q000378:metabolism; D011773:Pyruvates / Q000378:metabolism; D019289:Pyruvic Acid; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
chemical_list: D004798:Enzymes; D007202:Indicators and Reagents; D007773:Lactates; D011773:Pyruvates; D009243:NAD; D019344:Lactic Acid; D019289:Pyruvic Acid; D007770:L-Lactate Dehydrogenase