title: Stability of SM-7338, a new carbapenem in mediums recommended for the susceptibility testing of anaerobic bacteria and gonococci.
abstract: The stability of SM-7338 was compared to that of imipenem in media used for susceptibility testing anaerobic bacteria and Neisseria gonorrhoeae. SM-7338 was more stable in all media than imipenem. For tests with anaerobic bacteria, the broth-disk elution (in thioglycolate) and other methods recommended by the National Committee for Clinical Laboratory Standards can be accurately used for SM-7338. However, the cysteine content of IsoVitaleX (25.9 g/L) supplement inactivates SM-7338 (20-fold reduction) in gonococcal susceptibility test systems with GC agar base. A cysteine-free supplement would be advised for tests with the carbapenems and clavulanic acid. The SM-7338 disk diffusion test (10 micrograms) results were not significantly influenced by the inactivating substances in the media.
authors.affiliation: Clinical Microbiology Institute, Tualatin, Oregon 97062.
authors.forename: R N
authors.initials: RN
authors.lastname: Jones
authors.forename: R V
authors.initials: RV
authors.lastname: Gardiner
mesh_terms: D001421:Bacteria, Anaerobic / Q000187:drug effects; D003470:Culture Media; D004355:Drug Stability; D000077731:Meropenem; D008826:Microbial Sensitivity Tests / Q000379:methods*; D009344:Neisseria gonorrhoeae / Q000187:drug effects; D013845:Thienamycins / Q000494:pharmacology
chemical_list: D003470:Culture Media; D013845:Thienamycins; D000077731:Meropenem