title: Contamination of K-Sol corneal storage medium with Propionibacterium acnes.
abstract: K-Sol is a widely used corneal preservation medium. It has an excellent record for corneal storage and no previous report of contamination. During September 1988, on five of our K-Sol-stored donor rims Propionibacterium acnes grew. Cultures of unopened K-Sol also yielded P acnes. This report reviews previous contaminations of corneal storage media and the special characteristics of P acnes that enabled it to survive precautions taken against infection. The detection of P acnes was not obvious, and we emphasize the need for appropriate culturing of all donors, both aerobically and anaerobically, and close scrutiny of delayed inflammation in postoperative patients.
authors.affiliation: Department of Ophthalmology, Fitzsimons Army Medical Center, Aurora, Colo. 80045.
authors.forename: E A
authors.initials: EA
authors.lastname: Sieck
authors.forename: R W
authors.initials: RW
authors.lastname: Enzenauer
authors.forename: F M
authors.initials: FM
authors.lastname: Cornell
authors.forename: C
authors.initials: C
authors.lastname: Butler
mesh_terms: D002807:Chondroitin / Q000031:analogs & derivatives; D002809:Chondroitin Sulfates; D003315:Cornea; D016039:Corneal Transplantation; D003470:Culture Media; D004340:Drug Contamination; D005839:Gentamicins; D006531:HEPES; D006801:Humans; D010879:Piperazines; D011425:Propionibacterium acnes / Q000302:isolation & purification*; D014021:Tissue Preservation
chemical_list: D003470:Culture Media; D005839:Gentamicins; C051174:K-Sol preserving medium; D010879:Piperazines; D002807:Chondroitin; D002809:Chondroitin Sulfates; D006531:HEPES