Inhibitory effect of antibiotic cerulenin on the respiratory burst in phagocytes. I. Effects of cerulenin on active oxygen-generation and lipid metabolism in phagocytes.
The antibiotic cerulenin, a known inhibitor of fatty acid and sterol synthesis, inhibited bactericidal activity of mouse peritoneal macrophages and chemiluminescence (CL) response upon phagocytosis. The antibiotic also inhibited the CL response of human neutrophils upon exposure to various stimuli such as chemotactic peptide N-formylmethionylleucylphenylalanine (fMLP), calcium ionophore A23187 and Staphylococcal delta toxin. The loss of CL response of both types of cells was observed only after incubation of the cells with cerulenin for certain periods. The results of radioactive precursor incorporation suggest that lipid metabolism blocked by cerulenin affected in turn signal transduction across the cell membrane and inhibited CL production in these cells.
Nakata
M
M
Institute of Medical Science, University of Tokyo, Japan.
Tomita
T
T
Kanegasaki
S
S
D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D001427:Bacterial Toxins / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002569:Cerulenin / Q000494:pharmacology*; D005260:Female; D006801:Humans; D050356:Lipid Metabolism; D008163:Luminescent Measurements; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008809:Mice, Inbred C3H; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D010529:Peritoneal Cavity / Q000166:cytology; D010586:Phagocytes / Q000187:drug effects* / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects*; D015398:Signal Transduction
D000935:Antifungal Agents; D001427:Bacterial Toxins; C025971:staphylococcal delta toxin; D002569:Cerulenin; D000001:Calcimycin; D009240:N-Formylmethionine Leucyl-Phenylalanine; D010100:Oxygen