title: Expression of human immunodeficiency virus type 1 (HIV-1) gag antigens on the surface of a cell line persistently infected with HIV-1 that highly expresses HIV-1 antigens.
abstract: MT-4 cells persistently infected with human immunodeficiency virus type 1 (HIV-1) (MT-4/HIV-1) were recently isolated (K. Ikuta, C. Morita, M. Nakai, N. Yamamoto, and S. Kato, Japan. J. Cancer Res. (Gann), 79, 418-423, 1988). Mouse hybridoma cell clones producing monoclonal antibodies (MoAbs) to HIV-1 gag p24 and p18, and pol reverse transcriptase (RT) were isolated by using this MT-4/HIV-1 cell line for the screening of MoAb production by the immunofluorescence (IF) test. By indirect IF tests of acetone-fixed cells with these MoAbs, the IF intensities in MT-4/HIV-1 cells were found to be higher than those in the other HIV-1 infected cells, such as MOLT-4/HIV-1, HL-60/HIV-1, and U937/HIV-1 cells. Cell surface expression of the HIV-1 gag p24 and p18 antigens examined by IF and radioimmune techniques with these MoAbs revealed the p24 and p18 antigens to be expressed strongly on the cell surface of MT-4/HIV-1 cells and faintly on the cell surface of MOLT-4/HIV-1 cells, respectively. However, monoclonal antibody isolated in the present study failed to detect pol RT antigen on the surface of MT-4/HIV-1 cells. These results indicate that the gag p24 and p18 antigens are expressed, at least in part, on the surface of HIV-1-infected cells.
authors.affiliation: Department of Pathology, Osaka University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ikuta
authors.forename: C
authors.initials: C
authors.lastname: Morita
authors.forename: S
authors.initials: S
authors.lastname: Miyake
authors.forename: T
authors.initials: T
authors.lastname: Ito
authors.forename: M
authors.initials: M
authors.lastname: Okabayashi
authors.forename: K
authors.initials: K
authors.lastname: Sano
authors.forename: M
authors.initials: M
authors.lastname: Nakai
authors.forename: K
authors.initials: K
authors.lastname: Hirai
authors.forename: S
authors.initials: S
authors.lastname: Kato
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D015488:HIV Antigens / Q000032:analysis* / Q000096:biosynthesis; D016655:HIV Core Protein p24; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D006825:Hybridomas; D011233:Precipitin Tests; D012191:Retroviridae Proteins / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D015488:HIV Antigens; D016655:HIV Core Protein p24; D012191:Retroviridae Proteins