Monoclonal antibodies against bovine growth hormone.
Immunized spleen lymphocytes were fused with Balb/c mouse myeloma cells to obtain hybridoma cell line 4B-2, which produces monoclonal antibodies (McAb) against bovine growth hormone (bGH). Balb/c mice were injected with 4B-2 cells to produce ascites antibodies. The specific McAb in the ascites (about 10 mg/ml, subclass IgG1) was purified by immunoaffinity chromatography (IAC) on bGH-Sepharose 4B. This McAb cross-reacted with porcine growth hormone (pGH) but not with human (hGH), ovine (oGH), or fish (fGH) growth hormone. A 17 mg portion of purified McAb, coupled to the IAC affinity column was used to purify 1 mg of bGH. The bGH preparation retained its activity in rabbit liver receptor assay and in the tibia test following IAC.
Yu
Y M
YM
Institute of Developmental Biology, Academia Sinica, Beijing.
Yu
M Z
MZ
Shi
Y X
YX
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D002417:Cattle; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D013006:Growth Hormone / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D008862:Microsomes, Liver / Q000378:metabolism; D011817:Rabbits
D000911:Antibodies, Monoclonal; D013006:Growth Hormone