Kappa B-specific DNA binding proteins: role in the regulation of human interleukin-2 gene expression.
Transcriptional activation of the human interleukin-2 (IL-2) gene, like induction of the IL-2 receptor alpha (IL-2R alpha) gene and the type 1 human immunodeficiency virus (HIV-1), is shown to be modulated by a kappa B-like enhancer element. Mutation of a kappa B core sequence identified in the IL-2 promoter (-206 to -195) partially inhibits both mitogen- and HTLV-I Tax-mediated activation of this transcription unit and blocks the specific binding of two inducible cellular factors. These kappa B-specific proteins (80 to 90 and 50 to 55 kilodaltons) similarly interact with the functional kappa B enhancer present in the IL-2R alpha promoter. These data suggest that these kappa B-specific proteins have a role in the coordinate regulation of this growth factor-growth factor receptor gene system that controls T cell proliferation.
Hoyos
B
B
Mount Sinai Medical Center, Department of Microbiology, New York, NY 10029.
Ballard
D W
DW
Böhnlein
E
E
Siekevitz
M
M
Greene
W C
WC
D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D005814:Genes, Viral; D015497:HIV-1 / Q000235:genetics; D015486:HTLV-I Antigens / Q000494:pharmacology; D006801:Humans; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D007376:Interleukin-2 / Q000235:genetics*; D008970:Molecular Weight; D009154:Mutation; D010835:Phytohemagglutinins / Q000494:pharmacology; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis; D013601:T-Lymphocytes / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015534:Trans-Activators; D014157:Transcription Factors / Q000494:pharmacology; D014158:Transcription, Genetic; D014162:Transfection
D004268:DNA-Binding Proteins; D015486:HTLV-I Antigens; D007145:Immunoglobulin kappa-Chains; D007376:Interleukin-2; D010835:Phytohemagglutinins; D012333:RNA, Messenger; D015534:Trans-Activators; D014157:Transcription Factors; D004247:DNA; D013755:Tetradecanoylphorbol Acetate