title: [Cloning and expression of the determinant for resistance to a new class of tetracycline in Escherichia coli strains].
abstract: Tetracycline-resistance determinant of the plasmid pBS221 isolated from a Pseudomonas aeruginosa strain has been cloned on pUC19 vector plasmid. The determinant is expressed under aerobic and anaerobic conditions coding for two proteins: a 36 Kd protein conferring antibiotic resistance and 27 Kd repressor protein. The determinant is not homologous to tet-determinants of the known classes as shown by blot hybridization experiments. The determinant represents a new class--G. Determinants of the new class are widespread among Serratia marcescens strains.
authors.forename: O V
authors.initials: OV
authors.lastname: Parfenova
authors.forename: L A
authors.initials: LA
authors.lastname: Anisimova
authors.forename: A M
authors.initials: AM
authors.lastname: Boronin
mesh_terms: D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D006090:Gram-Negative Bacteria / Q000235:genetics; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000235:genetics; D013753:Tetracycline Resistance / Q000235:genetics*
vernacular_title: Klonirovanie i ékspressiia determinanta rezistentnosti k tetratsiklinnovogo klassa v shtammakh Escherichia coli.