A Ph1 chromosome positive B cell line expresses the fusion protein P 210.
We established a B lymphocyte line from bone marrow cultures of a patient with Philadelphia chromosome (Ph1) positive chronic myelogenous leukemia. The cell line (MB) is positive for Ph1 and has the phenotype of mature B lymphocytes transformed by the Epstein-Barr virus (EBV). When DNA was isolated and hybridized to specific cDNA probes, a rearranged immunoglobulin gene and EBV-sequences were detected. On Western blots of cell extracts, the novel fusion protein (P 210) was demonstrated with antisera against bcr and v-abl proteins. These results confirm the concept that B lymphocytes in CML can be involved in the malignant clone and demonstrate that these B cells express the fusion protein.
Munker
R
R
Medizinische Klinik III, Klinikum Grosshadern, München, Fed Rep of Germany.
Miller
C W
CW
Berenson
J
J
Dreazen
O
O
Koeffler
H P
HP
D000328:Adult; D001402:B-Lymphocytes / Q000032:analysis* / Q000473:pathology; D001752:Blast Crisis / Q000235:genetics / Q000473:pathology; D015139:Blotting, Southern; D015153:Blotting, Western; D002460:Cell Line; D016044:Fusion Proteins, bcr-abl; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics* / Q000473:pathology; D008297:Male; D009363:Neoplasm Proteins / Q000302:isolation & purification*; D011518:Proto-Oncogene Proteins / Q000302:isolation & purification*
D009363:Neoplasm Proteins; D011518:Proto-Oncogene Proteins; D016044:Fusion Proteins, bcr-abl