title: Analysis of the relationship between defective sperm function and the generation of reactive oxygen species in cases of oligozoospermia.
abstract: The ability of human spermatozoa to exhibit sperm-oocyte fusion in response to the ionophore, A23187, was examined in relation to the capacity of these cells to generate reactive oxygen species. In 70 fertile control donors, there was an overwhelming pattern of high levels of sperm-oocyte fusion associated with low levels of reactive oxygen species production. By contrast, 88% of the 74 oligozoospermic patients exhibited less than 25% oocyte penetration in response to A23187 and 58% exhibited no penetration whatsoever. Of the 40 oligozoospermic patients who failed to respond to A23187, nine had low levels of reactive oxygen species production in association with impaired liquefaction of seminal plasma. Of the remainder, 17 (55%) exhibited defective sperm function together with elevated production of reactive oxygen species. These observations, which are the first to describe a biochemical defect in the spermatozoa of oligozoospermic patients, may carry significant implications for the etiology and treatment of this condition.
authors.affiliation: MRC Unit of Reproductive Biology, Edinburgh.
authors.forename: R J
authors.initials: RJ
authors.lastname: Aitken
authors.forename: J S
authors.initials: JS
authors.lastname: Clarkson
authors.forename: T B
authors.initials: TB
authors.lastname: Hargreave
authors.forename: D S
authors.initials: DS
authors.lastname: Irvine
authors.forename: F C
authors.initials: FC
authors.lastname: Wu
mesh_terms: D000001:Calcimycin / Q000494:pharmacology*; D005260:Female; D005609:Free Radicals; D006801:Humans; D008297:Male; D009845:Oligospermia / Q000378:metabolism* / Q000503:physiopathology; D010100:Oxygen / Q000378:metabolism*; D013084:Sperm-Ovum Interactions / Q000187:drug effects*
chemical_list: D005609:Free Radicals; D000001:Calcimycin; D010100:Oxygen