Limiting dilution analysis of T cell unresponsiveness to mycobacteria in advanced disseminated tuberculosis.
Peripheral blood mononuclear cells from patients with advanced disseminated tuberculosis (Dis-TB) do not respond to purified protein derivative (PPD) measured as cell proliferation, lymphokine production and interleukin (IL)-2 receptor (Tac antigen) expression. Limiting dilution analysis revealed "multi-hit" curves and low frequencies of PPD-reactive T cells in cultures of Dis-TB, and "single-hit" curves and high frequencies of PPD-reactive T cells in cultures of patients with localized form of pulmonary tuberculosis. Moreover, a strict relationship between Tac antigen expression and ability of exogenous IL-2 to enhance bulk culture cell proliferation was observed in Dis-TB patients.
Gilardini Montani
M S
MS
Department of Cellular and Developmental Biology, University of Rome, Italy.
Del Gallo
F
F
Lombardi
G
G
Del Porto
P
P
Piccolella
E
E
Arienzo
F
F
Colizzi
V
V
D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D015375:Receptors, Interleukin-2 / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014373:Tuberculin / Q000276:immunology; D014374:Tuberculin Test; D014397:Tuberculosis, Pulmonary / Q000276:immunology*
D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D014373:Tuberculin; D007371:Interferon-gamma