title: Quantitative immunohistochemistry: standardization and possible application in research and surgical pathology.
abstract: We describe three different methods of calibration of quantitative immunohistochemistry, two of them based on self-made dye-agarose or antigen-agarose substrates. Quantification of immunostained objects (immunosignals) can be done by (1) microscope photometry (plug method) or scanning-photometry; (2) and densitometry of a television transformed microscope image. Both approaches are subjected to the different methods of calibration and three examples of application.
authors.affiliation: Pathologisches Institut des Robert-Bosch-Krankenhauses, Stuttgart, FRG.
authors.forename: P
authors.initials: P
authors.lastname: Fritz
authors.forename: J
authors.initials: J
authors.lastname: Hönes
authors.forename: D
authors.initials: D
authors.lastname: Lutz
authors.forename: H
authors.initials: H
authors.lastname: Multhaupt
authors.forename: A
authors.initials: A
authors.lastname: Mischlinski
authors.forename: A
authors.initials: A
authors.lastname: Dörrer
authors.forename: P
authors.initials: P
authors.lastname: Schwarzmann
authors.forename: H V
authors.initials: HV
authors.lastname: Tuczek
authors.forename: W
authors.initials: W
authors.lastname: Müller
mesh_terms: D001119:Arginase / Q000032:analysis; D001172:Arthritis, Rheumatoid / Q000473:pathology; D001940:Breast / Q000473:pathology; D001943:Breast Neoplasms / Q000473:pathology; D002461:Cell Line, Transformed; D003720:Densitometry / Q000379:methods; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007124:Immunoenzyme Techniques; D007136:Immunoglobulins / Q000032:analysis; D007150:Immunohistochemistry / Q000379:methods*; D010783:Photometry / Q000379:methods; D011960:Receptors, Estrogen / Q000032:analysis; D013583:Synovial Membrane / Q000473:pathology
chemical_list: D007136:Immunoglobulins; D011960:Receptors, Estrogen; D001119:Arginase