title: Human cysticercosis and taeniasis: molecular approaches for specific diagnosis and parasite identification.
abstract: The construction and antibody screening of Taenia solium cDNA libraries, generated in the Escherichia coli bacteriophage lambda gt11, with the identification of clones putatively expressing antigen B, T. solium-specific and other antigens is described. Lysogens were produced from a number of selected clones and beta-galactosidase fusion peptides ranging in Mr of approximately 135,000-150,000 were demonstrated. These proteins were shown by immunoblotting to be reactive with a pool of sera from cysticercotic patients originally used in the cDNA library screening. We report a method whereby Taenia (T. saginata and T. pisiformis) eggs can be detected with high sensitivity in a specific DNA dot-blot hybridisation assay using total parasite DNA as probe. We show also that intra-specific DNA variability occurs in T. solium isolates obtained from different geographical areas and discuss the potential significance of this heterogeneity.
authors.affiliation: Department of Pure and Applied Biology, Imperial College of Science and Technology, London, U.K.
authors.forename: D P
authors.initials: DP
authors.lastname: McManus
authors.forename: E
authors.initials: E
authors.lastname: Garcia-Zepeda
authors.forename: A
authors.initials: A
authors.lastname: Reid
authors.forename: A K
authors.initials: AK
authors.lastname: Rishi
authors.forename: A
authors.initials: A
authors.lastname: Flisser
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics; D003551:Cysticercosis / Q000175:diagnosis*; D004247:DNA / Q000235:genetics; D006801:Humans; D013621:Taenia / Q000235:genetics; D013622:Taeniasis / Q000175:diagnosis*
chemical_list: D000947:Antigens, Helminth; D004247:DNA