Suppression of GABA-induced chloride current by 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2).
The effect of a convulsive agent, 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2), on the GABA-induced chloride current (ICl) in dissociated mouse sensory neurons was investigated using the whole cell clamp method. Trp-P-2 reversibly suppressed the GABA-induced ICl in a dose-dependent manner. The IC50 for Trp-P-2 on the ICl evoked by 3 microM GABA was 11.1 microM. Ro15-1788 had no effect on the suppressive action of Trp-P-2. These results suggest that the observed effect of Trp-P-2 is mediated by its action as an antagonist at GABAA receptors.
Kanai
Y
Y
Department of Hygiene and Preventive Medicine, Faculty of Medicine, University of Tokyo, Japan.
Aosaki
T
T
Wada
O
O
Manabe
S
S
D000409:Alanine / Q000494:pharmacology; D000818:Animals; D002243:Carbolines / Q000494:pharmacology*; D002712:Chlorides / Q000378:metabolism*; D005442:Flumazenil / Q000494:pharmacology; D018756:GABA Antagonists; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D009118:Muscimol / Q000494:pharmacology; D009475:Neurons, Afferent / Q000187:drug effects* / Q000378:metabolism; D013654:Taurine / Q000494:pharmacology
D002243:Carbolines; D002712:Chlorides; D018756:GABA Antagonists; D013654:Taurine; D009118:Muscimol; D005442:Flumazenil; C019133:3-amino-1-methyl-5H-pyrido(4,3-b)indole; D000409:Alanine