title: Growth of gram-positive and gram-negative bacteria in platelet concentrates.
abstract: In 1986 the allowable platelet storage time was reduced from 7 to 5 days because of a recent increase in septic deaths associated with platelet transfusion. In this study, the growth curves of two gram-positive and two gram-negative organisms in platelets stored for 7 days in CLX and PL-732 bags were evaluated. Platelets in CLX bags were inoculated with 10(1), 10(2), and 10(3) organisms and 10(2) organisms were introduced into PL-732 bags. Test organisms were inoculated into trypticase soy broth as a control. All four bacteria grew rapidly in trypticase soy broth, reaching 10(9) organisms per mL within 48 hours. In both CLX and PL-732 bags, the growth pattern of gram-positive organisms was generally logarithmic during the first few days of storage. A concentration of 10(8) organisms per mL was present by Day 3 or 4, after which further proliferation was inhibited by the high density of bacteria in the platelets. In PL-732 bags, the proliferation of gram-negative organisms followed a pattern similar to that of the gram-positive bacteria. However, gram-negative organisms grew less well in CLX bags.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: American Red Cross Blood Services, Rochester, New York.
authors.forename: A
authors.initials: A
authors.lastname: Punsalang
authors.forename: J M
authors.initials: JM
authors.lastname: Heal
authors.forename: P J
authors.initials: PJ
authors.lastname: Murphy
mesh_terms: D000328:Adult; D001792:Blood Platelets / Q000382:microbiology*; D001793:Blood Preservation; D013293:Enterococcus faecalis / Q000254:growth & development*; D004926:Escherichia coli / Q000254:growth & development*; D006801:Humans; D007711:Klebsiella pneumoniae / Q000254:growth & development*; D008297:Male; D013203:Staphylococcal Infections / Q000209:etiology; D013211:Staphylococcus aureus / Q000254:growth & development*; D013696:Temperature; D013997:Time Factors; D065227:Transfusion Reaction