Calcium phosphate-mediated transfection alters metallothionein gene expression in response to Cd2+ and Zn2+.
The level of expression of a transfected metallothionein (MT)-IGcat fusion gene in response to cadmium differed from that of the endogenous MT-IG gene. Atomic absorption analysis indicated that the total cellular content of cadmium and zinc increased upon calcium phosphate-mediated transfection. Thus, changes in the influx/efflux of metals may regulate the level of MT gene expression.
Foster
R
R
Department of Biological Sciences, University of Calgary, Alberta, Canada.
Olsson
P E
PE
Gedamu
L
L
D002104:Cadmium / Q000378:metabolism; D000001:Calcimycin / Q000494:pharmacology; D002130:Calcium Phosphates / Q000494:pharmacology; D002460:Cell Line; D003001:Cloning, Molecular; D003300:Copper / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D008668:Metallothionein / Q000235:genetics*; D014162:Transfection / Q000187:drug effects; D015032:Zinc / Q000378:metabolism
D002130:Calcium Phosphates; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; D002104:Cadmium; D000001:Calcimycin; C485838:calcium phosphate, monobasic, anhydrous; D003300:Copper; D008668:Metallothionein; C020243:calcium phosphate; D015032:Zinc; C485829:calcium phosphate, dibasic, anhydrous