title: Gas exchange properties of goat hemoglobins A and C.
abstract: Hypoxic or anemic goats with the A hemoglobin genotype switch to the production of hemoglobin C, resulting in a reduced blood oxygen affinity. However, the physiologic consequences of this switch are not clear. We therefore studied the gas exchange properties of the two hemoglobin types. We found that purified hemoglobins A and C have very similar oxygen affinities and H+ Bohr effects, but in the presence of CO2, the affinity of hemoglobin C is substantially less than that of hemoglobin A. That this is not a nonspecific ionic effect is suggested by identical effects of NaCl on O2 binding to the two proteins and by a 2-fold higher capacity of hemoglobin C to bind CO2. The data can be explained by a class of CO2 binding sites in the beta C chain whose affinity is much higher than that of either of the primary sites or of those in Hb A. Our results suggest that in hemoglobin C-containing red cells CO2 acts as a potent allosteric effector, analogous to the role played by 2,3-diphosphoglycerate in human red blood cells. Goat hemoglobin C may have advantages over hemoglobins A or B in O2 transport under hypoxic conditions or in anemia.
authors.affiliation: Division of Blood Research, Letterman Army Institute of Research, San Francisco, California 94129.
authors.forename: R M
authors.initials: RM
authors.lastname: Winslow
authors.forename: M L
authors.initials: ML
authors.lastname: Swenberg
authors.forename: J
authors.initials: J
authors.lastname: Benson
authors.forename: M
authors.initials: M
authors.lastname: Perrella
authors.forename: L
authors.initials: L
authors.lastname: Benazzi
mesh_terms: D000818:Animals; D001784:Blood Gas Analysis; D002245:Carbon Dioxide / Q000097:blood / Q000502:physiology; D003720:Densitometry; D006041:Goats; D006441:Hemoglobin A / Q000032:analysis* / Q000378:metabolism; D006444:Hemoglobin C / Q000032:analysis* / Q000378:metabolism; D008954:Models, Biological; D010100:Oxygen / Q000097:blood / Q000502:physiology; D010313:Partial Pressure; D012965:Sodium Chloride
chemical_list: D002245:Carbon Dioxide; D012965:Sodium Chloride; D006444:Hemoglobin C; D006441:Hemoglobin A; D010100:Oxygen