A role for class I MHC antigens in responses of activated B lymphocytes.
Expression of the H-2 D protein was increased over 10-fold in B cells activated by IL-4 IL-5, IFN-gamma or LPS. Differences in the regulation of H-2 D expression and in growth rates were detected during lymphokine-triggered responses of subpopulations of splenic B cells. The enhancement of H-2 D expression on activated B cells was regulated at the transcriptional level and required synthesis of an intermediate cellular protein(s). B cell growth and differentiation were inhibited by antibodies to the H-2 D Ag suggesting a signaling function for the class I molecules on activated B lymphocytes. In T cells, a similar elevation of H-2 D expression occurred after stimulation with IL-5 or Con A.
Southern
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Department of Biology, University of California, San Diego, La Jolla 92093.
Dutton
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000502:physiology; D001667:Binding, Competitive; D002454:Cell Differentiation; D006183:H-2 Antigens / Q000032:analysis / Q000276:immunology*; D007371:Interferon-gamma / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology; D015847:Interleukin-4; D015848:Interleukin-5; D007378:Interleukins / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D013154:Spleen; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D006183:H-2 Antigens; D007376:Interleukin-2; D015848:Interleukin-5; D007378:Interleukins; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D015847:Interleukin-4; D007371:Interferon-gamma