title: Direct effects of estrogen on c-fos and c-myc protooncogene expression and cellular proliferation in human breast cancer cells.
abstract: Estrogen induces DNA synthesis to a lesser degree, but with similar kinetics compared to insulin-like growth factors when added to quiescent MCF7 human breast cancer cells. Moreover, this steroid rapidly induces expression of the growth-related c-fos and c-myc protooncogenes. The induction was shown to be a direct effect, independent of protein synthesis. These results demonstrate that no growth factor production is involved in estrogen-induced protooncogene expression, and that these direct effects on gene expression may be an important step in estrogen-induced mitogenesis.
authors.affiliation: Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Utrecht.
authors.forename: B
authors.initials: B
authors.lastname: van der Burg
authors.forename: A J
authors.initials: AJ
authors.lastname: van Selm-Miltenburg
authors.forename: S W
authors.initials: SW
authors.lastname: de Laat
authors.forename: E J
authors.initials: EJ
authors.lastname: van Zoelen
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism*; D002455:Cell Division; D004261:DNA Replication; D004958:Estradiol / Q000502:physiology*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007328:Insulin / Q000502:physiology; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016760:Proto-Oncogene Proteins c-fos; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger / Q000096:biosynthesis; D013997:Time Factors; D014407:Tumor Cells, Cultured
chemical_list: D007328:Insulin; D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D004958:Estradiol