Identification of a yeast protein with properties similar to those of the immunoglobulin heavy-chain enhancer-binding protein NF-muE3.
We demonstrate that Saccharomyces cerevisiae cells possess a 33-41-kilodalton protein with DNA-binding properties remarkably similar to those of the immunoglobulin enhancer-binding protein NF-muE3. We further show that the muE3-binding site functions as an upstream activating sequence in yeast cells, stimulating transcription from a truncated CYC1 promoter. These data suggest that the yeast protein, designated YEB-3, and NF-muE3 are functionally related and perhaps evolutionarily conserved.
Beckmann
H
H
Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, Philadelphia 19104-6072.
Kadesch
T
T
D000818:Animals; D001483:Base Sequence; D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins / Q000032:analysis*; D003850:Deoxyribonuclease I; D004742:Enhancer Elements, Genetic; D005656:Fungal Proteins / Q000032:analysis*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D008745:Methylation; D051379:Mice; D008970:Molecular Weight; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014157:Transcription Factors / Q000032:analysis; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D007143:Immunoglobulin Heavy Chains; D014157:Transcription Factors; D003850:Deoxyribonuclease I