Purification of ovine prolactin by affinity chromatography with monoclonal antibodies.
Five monoclonal antibodies (McAbs) against ovine prolactin (oPRL) were obtained by the lymphocyte hybridoma technique. One low-affinity McAb with (2D2) was purified by hydroxylapatite chromatography. The 2D2 was immobilized on Sepharose 4B with epichlorohydrin to produce an immunosorbent column, which was used to purify oPRL from a crude preparation by a single-step procedure.
Yang
H Y
HY
Bethune University of Medical Sciences, Changchun.
Zhou
B G
BG
Sun
L Q
LQ
Yang
J P
JP
Mai
Y C
YC
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D008807:Mice, Inbred BALB C; D011388:Prolactin / Q000276:immunology / Q000302:isolation & purification*; D012756:Sheep
D000911:Antibodies, Monoclonal; D011388:Prolactin