Isolation of allergenically active glycoprotein from Prosopis juliflora pollen.
An allergenically active glycoprotein was homogeneously isolated from the aqueous extract of Prosopis juliflora pollen by ConA-Sepharose affinity chromatography. The molecular weight of this glycoprotein was 20,000 dalton, determined by gel filtration and SDS-PAGE. This fraction showed a total carbohydrate concentration of 25%. The purified glycoprotein revealed immunochemically most antigenic or allergenic and demonstrated homogeneous after reaction with P. juliflora pollen antiserum, characterized by gel diffusion, Immunoelectrophoresis and Radioallergosorbent test.
Thakur
I S
IS
Department of Environmental Sciences, G.B. Pant Univ. of Agric. & Tech., Nainital, India.
D000485:Allergens / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000276:immunology / Q000302:isolation & purification*; D007106:Immune Sera / Q000276:immunology; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D010936:Plant Extracts / Q000032:analysis; D011058:Pollen / Q000032:analysis*
D000485:Allergens; D006023:Glycoproteins; D007106:Immune Sera; D010936:Plant Extracts