Gonadotropin stimulated protein phosphorylation in porcine granulosa cells.
Treatment of granulosa cells with luteinizing hormone (LH) or follicle-stimulating hormone (FSH) stimulated the phosphorylation of a 58,000 molecular weight protein found in the 900 x g pellet. The phosphorylation of this protein was rapid, being significant at 1 min. LH treatment for 30 min induced greater phosphorylation of this protein than did FSH. LH and FSH also appeared to stimulate the phosphorylation of different 900 x g pellet proteins. Since both are known to utilize cAMP as a second messenger, the finding of these unique gonadotropin-induced phosphorylations may point to an additional regulatory mechanism.
Myers
L M
LM
Department of Physiology and Biophysics, University of Oklahoma Health Sciences Center, Oklahoma City 73190.
Kling
O R
OR
D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D006107:Granulosa Cells / Q000378:metabolism* / Q000648:ultrastructure; D007986:Luteinizing Hormone / Q000494:pharmacology*; D008970:Molecular Weight; D010750:Phosphoproteins / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism; D013552:Swine; D013997:Time Factors
D010750:Phosphoproteins; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone