title: The malate synthase gene of cucumber.
abstract: The complete sequences of a full-length cDNA clone and a genomic clone encoding the Cucumis sativus glyoxysomal enzyme malate synthase, have been determined. The sequences have enabled us to identify putative control regions at the 5' end of the gene, three introns, and possible alternative polyadenylation sites at the 3' end. The deduced amino acid sequence predicts a polypeptide of 64,961 molecular weight, which has 48% identity with that of Escherichia coli. Comparison of the sequence of malate synthase from cucumber with that from E. coli and with other glyoxysomal and peroxisomal enzymes, shows that a conserved C-terminal tripeptide is a common feature of those enzymes imported into microbodies.
authors.affiliation: Department of Botany, University of Edinburgh, Scotland, UK.
authors.forename: I A
authors.initials: IA
authors.lastname: Graham
authors.forename: L M
authors.initials: LM
authors.lastname: Smith
authors.forename: J W
authors.initials: JW
authors.lastname: Brown
authors.forename: C J
authors.initials: CJ
authors.lastname: Leaver
authors.forename: S M
authors.initials: SM
authors.lastname: Smith
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D008292:Malate Synthase / Q000235:genetics*; D008969:Molecular Sequence Data; D010944:Plants / Q000201:enzymology / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014158:Transcription, Genetic; D014675:Vegetables / Q000201:enzymology / Q000235:genetics
chemical_list: D004247:DNA; D008292:Malate Synthase