In situ hybridization using biotin-labeled oligonucleotides: probe labeling and procedures for mRNA detection.
Methods for non-radioactive in situ hybridization are rapidly progressing in refinement and application. In this overview, we present our experiences with the use of synthetic oligodeoxynucleotides, labeled with biotin- or digoxigenin-tagged nucleotides by the terminal transferase method.
Larsson
L I
LI
Research Center for Medical Biotechnology, State Serum Institute, Copenhagen, Denmark.
D000818:Animals; D001710:Biotin; D004247:DNA / Q000235:genetics; D006801:Humans; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides; D012333:RNA, Messenger / Q000235:genetics*
D009841:Oligonucleotides; D012333:RNA, Messenger; D001710:Biotin; D004247:DNA