Stage-specific gene expression in early chick embryo.
Inhibition of DNA replication by aphidicolin in the chick morula interferes with its progression to a normal blastula and prevents induction of the first morphogenetic cell movements of primitive streak formation. Embryos in aphidicolin synthesize some polypeptides typical of blastula but do not display all the characteristic features of morula to blastula transition. Inhibition of DNA replication interferes with the sequential synthesis of maternally coded polypeptides and with the activation of the embryonic genome in the chick embryo.
Zagris
N
N
Department of Biology, University of Patras, Greece.
Matthopoulos
D
D
D000818:Animals; D016590:Aphidicolin; D001755:Blastocyst / Q000187:drug effects / Q000378:metabolism*; D002449:Cell Aggregation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002642:Chick Embryo; D002970:Cleavage Stage, Ovum / Q000378:metabolism*; D004257:DNA Polymerase II / Q000037:antagonists & inhibitors; D004261:DNA Replication / Q000187:drug effects; D004224:Diterpenes / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D006651:Histocytochemistry; D009024:Morphogenesis; D009028:Morula / Q000187:drug effects / Q000378:metabolism*; D014176:Protein Biosynthesis
D004224:Diterpenes; D016590:Aphidicolin; D004257:DNA Polymerase II