A 4-day chemosensitivity assay in vitro reliably predicts clinical response of patients with acute leukemia.
To assess the individual chemosensitivity of human acute leukemias to anticancer agents, a rapid in vitro assay based on 125[I]-5-iodo-2'-deoxyuridine (125IUdR) incorporation into the DNA of neoplastic cells was tested. It was found that: a) the assay reflects the higher in vivo responsiveness of acute lymphoid leukemias with respect to acute non-lymphoid leukemias; b) samples from responder patients were more sensitive in vitro to antileukemic agents than were samples from non-responder patients; c) the assay in this study had an 86% accuracy in predicting response and a 90% accuracy in predicting resistance.
Barzi
A
A
Lepri
E
E
Menconi
E
E
Nocentini
G
G
Liberati
M
M
Schippa
M
M
D000293:Adolescent; D000328:Adult; D000368:Aged; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D002648:Child; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy*; D008875:Middle Aged; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000188:drug therapy*; D011237:Predictive Value of Tests; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents