[The effect of synthetic proteinase inhibitors of the level of human recombinant proinsulin production, secreted by a genetically engineered strain of Bacillus subtilis].
Influence of O,O,-diethyl-1-(N-alpha-hydrohexafluoroisobutyryl)amino-1- methylpropylphosphonate and O,O-diisobutyl-1-[2-(ethoxycarbonyl)aminoperfluoroprop-2-yl] -1- methylpropylphosphonate on the level of production of human proinsulin secreted by a genetically engineered culture Bacillus subtilis AJ 73 (pBINS1.0.) has been studied. The above phosphonates, being non-toxic for microorganisms, reduced degradation of proinsulin by serine proteinases.
Parfenova
E V
EV
Popov
D G
DG
Novikov
A A
AA
Sterkin
V E
VE
Strongin
A Ia
AIa
Aksinenko
A Iu
AIu
Korenchenko
O V
OV
Fetisov
V I
VI
Martynov
I V
IV
Sokolov
V B
VB
D000818:Animals; D001412:Bacillus subtilis / Q000378:metabolism*; D005818:Genetic Engineering; D006801:Humans; D007329:Insulin Antagonists; D011384:Proinsulin / Q000096:biosynthesis*; D011480:Protease Inhibitors / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000096:biosynthesis; D013552:Swine
D007329:Insulin Antagonists; D011480:Protease Inhibitors; D011994:Recombinant Proteins; D011384:Proinsulin
Vliianie sinteticheskikh ingibitorov proteinaz na uroven' produktsii rekombinantnogo proinsulina cheloveka, sekretiruemogo genno- inzhenernym shtammom Bacillus subtilis.