title: Cytoplasmic pH in cultured porcine thyroid cells: alkalinization by Ca2+.
abstract: The role of calcium in cytoplasmic pH (pHi) changes was studied using 2',7'-bis(2-carboxyethyl)-5-(and 6-)carboxyfluorescein, an internalized fluorescent pH indicator, in cultured porcine thyroid cells. The Ca2+ ionophores A23187 and ionomycin stimulated thyroid cell alkalinization. An increase in cytoplasmic free calcium resulted in activation of Na+/H+ exchange which alkalinized the cells.
authors.affiliation: Department of Gerontology, School of Medicine, Shinshu University, Nagano-ken, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Takasu
authors.forename: I
authors.initials: I
authors.lastname: Komiya
authors.forename: Y
authors.initials: Y
authors.lastname: Nagasawa
authors.forename: T
authors.initials: T
authors.lastname: Asawa
authors.forename: Y
authors.initials: Y
authors.lastname: Shimizu
authors.forename: T
authors.initials: T
authors.lastname: Yamada
mesh_terms: D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism*; D004987:Ethers / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D015759:Ionomycin; D007476:Ionophores / Q000494:pharmacology; D012964:Sodium / Q000378:metabolism / Q000494:pharmacology; D013552:Swine; D013961:Thyroid Gland / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
chemical_list: D004987:Ethers; D007476:Ionophores; D000001:Calcimycin; D015759:Ionomycin; D012964:Sodium; D002118:Calcium