Ribosomal protein S12 as a site for streptomycin resistance in Nicotiana chloroplasts.
A streptomycin resistant Nicotiana plastome mutant, X/strR6, was subjected to molecular analysis. In this mutant, a single nucleotide transition, C----T, in the chloroplast gene for ribosomal protein S12 alters codon 90 from proline to serine while the nucleotide sequence of the chloroplast 16 S rRNA gene is identical to that of the wild type. Mutant X/strR6 thus differs from several previously reported streptomycin resistant chloroplast mutants which are altered in the gene for 16 S rRNA.
Galili
S
S
Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel.
Fromm
H
H
Aviv
D
D
Edelman
M
M
Galun
E
E
D001483:Base Sequence; D002736:Chloroplasts / Q000187:drug effects*; D003001:Cloning, Molecular; D003062:Codon; D004351:Drug Resistance / Q000235:genetics; D005091:Exons; D009154:Mutation; D010940:Plant Proteins / Q000235:genetics*; D010947:Plants, Toxic; D011392:Proline / Q000235:genetics; D012269:Ribosomal Proteins / Q000235:genetics*; D012694:Serine / Q000235:genetics; D013307:Streptomycin / Q000494:pharmacology*; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics*
D003062:Codon; D010940:Plant Proteins; D012269:Ribosomal Proteins; C034212:ribosomal protein S12; D012694:Serine; D011392:Proline; D013307:Streptomycin