title: Ultrastructural localization of Cu, Zn-SOD in hepatocytes of patients with various liver diseases.
abstract: The ultrastructural localization of copper, zinc-superoxide dismutase (Cu, Zn-SOD) in the liver of patients with acute hepatitis, chronic hepatitis, liver cirrhosis and alcoholic fatty liver was studied by means of the indirect immunoperoxidase technique. In hepatocytes Cu, Zn-SOD was found to be localized in perinuclear cisternae, rough endoplasmic reticulum (rER), vesicles and Golgi apparatus. The Cu, Zn-SOD was also detected around the lipid droplets in hepatocytes as well as on the cytoplasmic membrane in cases of liver cirrhosis. These findings suggest that Cu, Zn-SOD is produced in the rER in hepatocytes and protects the cells from cellular injury caused by superoxide anion radical in various disorders of the liver.
authors.affiliation: Second Department of Internal Medicine, Yamagata University, School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Saito
authors.forename: H
authors.initials: H
authors.lastname: Shinzawa
authors.forename: H
authors.initials: H
authors.lastname: Togashi
authors.forename: H
authors.initials: H
authors.lastname: Wakabayashi
authors.forename: K
authors.initials: K
authors.lastname: Ukai
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
authors.forename: M
authors.initials: M
authors.lastname: Ishikawa
authors.forename: M
authors.initials: M
authors.lastname: Dobashi
authors.forename: Y
authors.initials: Y
authors.lastname: Imai
mesh_terms: D004721:Endoplasmic Reticulum / Q000201:enzymology; D006505:Hepatitis / Q000201:enzymology / Q000473:pathology; D006651:Histocytochemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D008099:Liver / Q000201:enzymology / Q000473:pathology* / Q000648:ultrastructure; D008103:Liver Cirrhosis / Q000201:enzymology / Q000473:pathology; D008107:Liver Diseases / Q000201:enzymology / Q000473:pathology*; D008854:Microscopy, Electron; D016253:Microscopy, Immunoelectron; D013194:Staining and Labeling; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D013482:Superoxide Dismutase