Recombinant interferon-gamma induces HLA-DR expression on human corneal epithelial and endothelial cells in vitro: a preliminary report.
The effect of interferon-gamma on the expression in situ of major histocompatibility complex (MHC) products in human corneas was studied in vitro. Incubation for four days with 5 or 50 mg/l of Escherichia coli-derived recombinant human interferon-gamma resulted in the appearance de novo of MHC class II or HLA-DR antigens on variable numbers of corneal epithelial cells as well as on corneal endothelium, whereas it had no effect on the expression of MHC class I or HLA-ABC antigens. These results may help to explain the mechanism underlying the expression of HLA-DR antigens on corneal and limbal epithelium in various inflammatory eye diseases.
el-Asrar
A M
AM
Department of Ophthalmology, Mansoura University Hospital, Egypt.
van den Oord
J J
JJ
Billiau
A
A
Desmet
V
V
Emarah
M h
Mh
Missotten
L
L
D002478:Cells, Cultured; D003315:Cornea / Q000187:drug effects* / Q000276:immunology; D004728:Endothelium, Corneal / Q000187:drug effects / Q000276:immunology; D004848:Epithelium / Q000187:drug effects / Q000276:immunology; D006684:HLA-DR Antigens / Q000032:analysis*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D011994:Recombinant Proteins
D006684:HLA-DR Antigens; D011994:Recombinant Proteins; D007371:Interferon-gamma