[Studies of the origin of false positive reactions in the serodiagnosis of contagious bovine pleuropneumonia].
Four groups of cattle were experimentally immunised by four mycoplasma species of "mycoides-like" group, Mycoplasma (M) capricolum, M. mycoides subsp. mycoides (LC), M. mycoides subsp. capri and M. species group 7 of LEACH (PG50). They were then bled weekly during 2 months to establish antibodies kinetics against homologous and heterologous antigens. The standard method of complement fixation test (CFT) used in Europe and a new ELISA test for diagnosis of contagious bovine pleuropneumonia were performed in comparison with passive haemagglutination test (PHA) against antigens used for experimental immunisation. Cross reactions obtained are rather equal to the degree of similitude between these mycoplasma species. With CFT-cross reactions are transitory and occur only while homologous titers are very high, particularly with "PG50" and the two caprine mycoides strains. ELISA results using a threshold of positivity of optical density of 0.20, were similar to that obtained with CFT except ELISA specificity is not so different from CFT one. This experimental model could explain some natural situations.
Poumarat
F
F
Laboratoire de Pathologie Bovine, Centre National d'Etudes Vétérinaires et Alimentaires, Lyon, France.
Perrin
M
M
Belli
P
P
Longchambon
D
D
Le Goff
C
C
Martel
J L
JL
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D005189:False Positive Reactions; D011002:Pleuropneumonia, Contagious / Q000175:diagnosis*; D012698:Serologic Tests
Recherche sur l'origine des fausses réactions positives dans le diagnostic sérologique de la péripneumonie contagieuse bovine.