A procedure to obtain long-lasting fluorescence in formaldehyde fixed tissues.
The authors made a study of the small intestine by immunofluorescence in 5% phosphate-buffered formaldehyde fixed tissues, using the Nairn (1976) technique associated with Evans blue (Fry and Wilkinson 1963). The ability of the sections to fluoresce has been maintained after a storage time of more than 4 years. Similar very good results were obtained when skin biopsies from pemphigus foliaceus patients were fixed in formaldehyde for two h and afterwards washed in 30% sucrose solution for 12 to 18 h.
Moreira
M A
MA
Faculty of Medicine, Federal University of Goiás, Goiânia, Brasil.
Barbieri
D
D
de Castro
L C
LC
D001706:Biopsy; D005455:Fluorescent Antibody Technique; D006652:Histological Techniques; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis*; D007413:Intestinal Mucosa / Q000166:cytology / Q000276:immunology* / Q000473:pathology; D007421:Intestine, Small / Q000166:cytology / Q000276:immunology / Q000473:pathology; D010392:Pemphigus / Q000276:immunology* / Q000473:pathology; D012867:Skin / Q000276:immunology* / Q000473:pathology; D013997:Time Factors
D007070:Immunoglobulin A; D007074:Immunoglobulin G