title: Bovine islets of Langerhans. Potential source for transplantation?
abstract: Two methods are described for large-scale isolation of islets from the bovine pancreas. With the single-endpoint technique the average number of islets isolated from a 73.3-g pancreas sample was 150,560, representing an estimated total islet volume of 0.391 ml. Pancreases processed by the continuous digestion-filtration method yielded an average of 246,229 islets from a 93.9-g pancreas sample, representing an estimated total islet volume of 0.458 ml. Isolated bovine pancreatic islets demonstrated a well-preserved endocrine cell ultrastructure, responded to acute stimulation with propionic acid, and temporarily reversed experimental diabetes in mice.
authors.affiliation: Medical Clinic III and Policlinic, Justus Liebig University, Giessen, Federal Republic of Germany.
authors.forename: B J
authors.initials: BJ
authors.lastname: Hering
authors.forename: D
authors.initials: D
authors.lastname: Romann
authors.forename: A
authors.initials: A
authors.lastname: Clarius
authors.forename: M
authors.initials: M
authors.lastname: Brendel
authors.forename: M
authors.initials: M
authors.lastname: Slijepcevic
authors.forename: R G
authors.initials: RG
authors.lastname: Bretzel
authors.forename: K
authors.initials: K
authors.lastname: Federlin
mesh_terms: D000818:Animals; D002417:Cattle; D002469:Cell Separation / Q000379:methods*; D016381:Islets of Langerhans Transplantation; D011422:Propionates / Q000494:pharmacology; D014183:Transplantation, Heterologous
chemical_list: D011422:Propionates; C029658:propionic acid