Induction and repair of cyclobuta-dithymidine photoproducts in hamster skin by solar-simulated UV radiation.
The induction and repair of cyclobuta-dithymidine (T less than greater than T) photoproducts in hamster skin was measured following exposure to artificial solar radiation. DNA extracted from irradiated hamster epidermis was analyzed by enzyme-linked immunosorbent assay (ELISA) using a monoclonal antibody specific for T less than greater than T photoproducts. Between 49% and 58% of T less than greater than T antibody binding sites were eliminated from DNA by 24 h after radiation exposures comparable with approximately 60 - 120 min of midday sunlight, whereas all detectable T less than greater than T binding sites were eliminated by 24 h after a radiation exposure comparable with 30 min of sunlight.
Strickland
P T
PT
Department of Environmental Health Sciences, Johns Hopkins University, School of Hygiene and Public Health, Baltimore, MD 21205.
Creasey
J S
JS
D000818:Animals; D006224:Cricetinae; D004247:DNA / Q000302:isolation & purification; D004260:DNA Repair; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D011740:Pyrimidine Dimers / Q000378:metabolism*; D012867:Skin / Q000528:radiation effects*; D014466:Ultraviolet Rays / Q000009:adverse effects*
D011740:Pyrimidine Dimers; C059224:cyclobutyldithymidine dimers; D004247:DNA