title: Complete amino acid sequence of pyrazine-binding protein from cow nasal mucosa.
abstract: The sequence is reported of the pyrazine-binding protein from cow olfactory/respiratory mucosa. The protein consists of 159 amino acids and clearly belongs to the retinol-binding protein family. It is most closely related to the urinary proteins from mice and rats and to the odour-binding protein from rat nasal epithelium. It is unique however, in that only one of the otherwise conserved features of the family is still present--namely a single tryptophan. Most surprisingly the protein contains no cysteine and, therefore, does not rely for its structural stability on the disulphide bond(s) present in other members of this group. A model for the protein has been constructed based on the co-ordinates of beta-lactoglobulin. From this, it is possible to identify residues which may line the binding site. The impression gained is of a much larger pocket than occurs with retinol-binding protein or beta-lactoglobulin. The character of the binding pocket remains essentially hydrophobic but with a significant reduction in its aromatic content and an increase in H-bonding side chains.
authors.affiliation: Department of Biochemistry, University of Leeds, England.
authors.forename: R
authors.initials: R
authors.lastname: Tirindelli
authors.forename: J N
authors.initials: JN
authors.lastname: Keen
authors.forename: A
authors.initials: A
authors.lastname: Cavaggioni
authors.forename: E E
authors.initials: EE
authors.lastname: Eliopoulos
authors.forename: J B
authors.initials: JB
authors.lastname: Findlay
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000302:isolation & purification*; D002417:Cattle; D003545:Cysteine / Q000032:analysis; D004220:Disulfides / Q000032:analysis; D010450:Endopeptidases; D006860:Hydrogen Bonding; D006868:Hydrolysis; D008666:Metalloendopeptidases; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009297:Nasal Mucosa / Q000032:analysis*; D010084:Oxidation-Reduction; D010434:Pepsin A; D010446:Peptide Fragments / Q000302:isolation & purification; D018035:Receptors, Odorant
chemical_list: D002352:Carrier Proteins; D004220:Disulfides; D010446:Peptide Fragments; D018035:Receptors, Odorant; C045648:odorant-binding protein; D010450:Endopeptidases; D010434:Pepsin A; D008666:Metalloendopeptidases; C039830:peptidyl-Lys metalloendopeptidase; D003545:Cysteine