Nuclear GTP-binding proteins of Swiss 3T3 cells.
The GTP-binding proteins of Swiss 3T3 cell nuclei were analyzed by filter binding assay and UV cross-linking analysis. The results showed the presence of multiple GTP-binding proteins in the nuclei. Scatchard analysis revealed that the Kd value for GTP binding to high-affinity components was 69 nM, that to low-affinity components being 2.7 microM. The GTP-binding activities of some nuclear proteins were found to change significantly in response to the growth conditions of the cells. During culture of cells in medium without serum, the GTP-binding activity of a 140 kDa protein clearly decreased, whereas that of a 40 kDa protein increased.
Takeda
S
S
Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Sugiyama
H
H
Natori
S
S
Sekimizu
K
K
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D002478:Cells, Cultured; D019204:GTP-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D008970:Molecular Weight; D013379:Substrate Specificity
D006160:Guanosine Triphosphate; D019204:GTP-Binding Proteins