Recombinant mouse interferon-gamma is not chemotactic for macrophages or neutrophils.
Recent evidence implicates interferon-gamma (IFN-gamma) in resistance to infection with the facultative intracellular bacterium, Listeria monocytogenes. Reports showing that inflammatory macrophages and neutrophils are highly bactericidal for listeria suggest that IFN-gamma might act by directly or indirectly causing recruitment of these cells. The purpose of the experiments described here was to test whether recombinant mouse IFN-gamma is chemotactic for macrophages or neutrophils in vivo or in vitro. In vivo experiments showed rIFN-gamma to have no ability to cause recruitment of inflammatory neutrophils or macrophages into the peritoneal cavities of mice. When tested in vitro, rIFN-gamma also did not induce migration of inflammatory neutrophils or macrophages through cellulose nitrate filters in a chemotaxis assay. The data indicate that rIFN-gamma has no direct or indirect chemotactic activity in vivo or in vitro for mouse macrophages or neutrophils.
Canono
B P
BP
Department of Medicine, National Jewish Center for Immunology and Respiratory Medicine, Denver, CO 80206.
Middleton
M H
MH
Campbell
P A
PA
D000818:Animals; D002633:Chemotaxis / Q000187:drug effects*; D005122:Exudates and Transudates / Q000166:cytology; D005260:Female; D007371:Interferon-gamma / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009504:Neutrophils / Q000187:drug effects*; D010529:Peritoneal Cavity / Q000166:cytology; D010586:Phagocytes / Q000187:drug effects; D011994:Recombinant Proteins
D011994:Recombinant Proteins; D007371:Interferon-gamma