title: Autoimmune mice and stimulated normal mice make lambda 1 with increased V region diversity.
abstract: Previous studies of the genetic bases of murine SLE have defined gene segments that encode the H chain and the kappa L chain of anti-DNA, anti-Sm, and anti-IgG autoantibodies. As a result of these studies, the genetic origins of autoantibody H chains and kappa L chains are better understood, but little remains known about the genetic bases of autoantibody lambda-chains. Thus, we have analyzed serologically the germ-line and somatic origins of lambda 1 L chains in antibodies of normal mice and in both antibodies and autoantibodies of autoimmune mice. This study finds an increased lambda 1 diversity in both Ag-stimulated mice and autoimmune mice. This study also finds that the lambda 1 L chains in antibodies of unstimulated normal mice have the gene segment-encoded variable region, V lambda 1. In contrast, additional genetic processes appear to make the lambda 1 V regions of antibodies in Ag-stimulated normal mice and the lambda 1 V regions of both antibodies and autoantibodies in autoimmune mice. The increased lambda 1 diversity that we found in both Ag-stimulated mice and autoimmune mice might be caused by mutational processes creating antibody diversities. Therefore, the same somatic processes might be able to make both antibody and autoantibody lambda 1 diversities.
authors.affiliation: Indiana University School of Medicine, Department of Microbiology and Immunology, Indianapolis 46223.
authors.forename: J H
authors.initials: JH
authors.lastname: Slack
authors.forename: L
authors.initials: L
authors.lastname: Hang
authors.forename: C
authors.initials: C
authors.lastname: Amundson
authors.forename: B L
authors.initials: BL
authors.lastname: Clevinger
authors.forename: F J
authors.initials: FJ
authors.lastname: Dixon
mesh_terms: D000818:Animals; D000916:Antibody Diversity; D001323:Autoantibodies / Q000096:biosynthesis* / Q000235:genetics; D001327:Autoimmune Diseases / Q000235:genetics / Q000276:immunology*; D001667:Binding, Competitive; D007146:Immunoglobulin lambda-Chains / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008814:Mice, Inbred NZB; D011817:Rabbits; D011863:Radioimmunoassay; D013045:Species Specificity
chemical_list: D001323:Autoantibodies; D007146:Immunoglobulin lambda-Chains