Role of autologous lymph node T cells in membrane expression of mu- or gamma-heavy chain isotype by malignant B NHL cells.
We investigated the possibility that T cells observed in lymph nodes involved by B-non-Hodgkin's lymphomas (B-NHL) may have a direct role in the expression of Mu- or Gamma- heavy chain isotype by autologous malignant B cells. T cells were separated from lymph nodes involved by B-NHL cells expressing either surface IgM (19 cases) or surface IgG (4 cases) and compared to peripheral blood T lymphocytes of healthy subjects (19 cases) for their ability to promote both IgG and IgM secretion in Cowan-activated normal B lymphocytes. The mean values of IgG/IgM ratios obtained under the influence of T cells associated with malignant B cells expressing either surface IgM or surface IgG were not statistically different to that obtained with the help of control T cells (0.60 and 0.62 versus 0.47, respectively). These results do not account for the hypothesis that autologous lymph node T cells may directly affect the expression of the heavy chain isotype by malignant B-NHL cells.
Bonnefoix
T
T
Department of Hematology, Hôpital A. Michallon, Grenoble, France.
Orfeuvre
H
H
Jacob
M C
MC
Piccinni
M P
MP
Sotto
J J
JJ
D001402:B-Lymphocytes / Q000276:immunology*; D002462:Cell Membrane / Q000378:metabolism; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000378:metabolism*; D007132:Immunoglobulin Isotypes / Q000378:metabolism; D007142:Immunoglobulin gamma-Chains / Q000378:metabolism*; D007148:Immunoglobulin mu-Chains / Q000378:metabolism*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000276:immunology* / Q000473:pathology; D008198:Lymph Nodes / Q000473:pathology; D008222:Lymphokines / Q000502:physiology; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology*
D007143:Immunoglobulin Heavy Chains; D007132:Immunoglobulin Isotypes; D007142:Immunoglobulin gamma-Chains; D007148:Immunoglobulin mu-Chains; D008222:Lymphokines; D011947:Receptors, Antigen, B-Cell