[Histochemical studies on the healing after partial excision of tongue. Regeneration and restoration of the muscular tissue].
After a partial excision of tongue of rats, regeneration and restoration of the muscular tissue in the vicinity of the exact region and the anterior regions treated, and the non-treated region, were particularly assessed from 3 days to 20 weeks after treatment and compared with those of controls histologically, enzyme-histochemically and immunohistochemically. The results were obtained as follows: 1. Histopathological findings 1) Atrophy or disappearance of the residual muscular fibers adjacent to the exact region was observed after treatment and an arrangement of the muscular fasciculi became more irregular as the fibers were gradually restored from 4 weeks, although regenerative fibers with a slight irregularity were seen from 3 days up to even 20 weeks after treatment. 2) Atrophy and the sparse arrangement of the muscular fibers of the anterior region were observed at the initial stage. However, they gradually recovered from 4 weeks after treatment and became similar to those of controls 8 weeks after treatment. 3) Atrophy of the non-treated region was occurred in muscular fibers 3 days after treatment and the muscular fasciculi with the irregular arrangement was dispersely observed, although the arrangement was gradually recovered from 2 weeks and became similar to that of controls from 4 weeks after treatment. 2. Enzyme histochemical findings 1) SDH (1) The activity in the residual muscular fibers adjacent to the exact region was weak at the initial stage, although the activity was restored to the control level from 4 weeks after treatment. The regenerated muscular fibers began to show less activity from 1 week, although the activity became similar to that of controls from 8 weeks after treatment. (2) The activity in the muscular fiber anterior to the region was less at the initial stage, but began to be restored from 2 weeks and recovered to the control level from 4 weeks after treatment. (3) The activity in the muscular fibers at the non-treated region became less 3 days and 1 week after treatment, but similar to that of controls from 2 weeks after treatment. 2) LDH: It showed similar changes to SDH. 3) ACP: (1) The weak activity was seen in the residual muscle fibers adjacent to the exact region, and the relatively strong activity was observed in a few muscular fibers 3 days and 1 week after treatment. (2) In the anterior region, a weak activity was seen at the initial stage, but not 2 weeks after treatment.(ABSTRACT TRUNCATED AT 400 WORDS)
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D000818:Animals; D005927:Glossectomy; D006651:Histocytochemistry; D009133:Muscular Atrophy / Q000503:physiopathology; D051381:Rats; D014059:Tongue / Q000201:enzymology / Q000503:physiopathology* / Q000601:surgery; D014945:Wound Healing