Immunoelectrophoretic analyses of antigens shared by the vesicular fluid and cyst wall of Taenia crassiceps and Taenia saginata metacestodes.
In this study, the antigenic mosaic of the vesicular fluid (VF) and hydrosoluble cyst-wall extract (CWE) of T. crassiceps (Tc; harvested from mice) and T. saginata (Ts) metacestodes was analyzed by combined precipitation maps developed in single and bidimensional immunoelectrophoresis against the respective rabbit antiserum. Host-serum proteins demonstrated by immunodiffusion within TcVF (albumin, transferrin, IgG, and another five noncharacterized proteins), TcCWE (albumin, IgG, and six additional unknown proteins), TsVF (albumin) and TsCWE (albumin and IgG) were removed by immunoaffinity chromatography prior to immunoelectrophoretic analysis. Neither TcVF nor TcCWE contained demonstrable amounts of mouse IgM and IgA. In TcVF a total of 18 and in TcCWE a total of 36 parasitic antigens were recognized by the corresponding antiserum. In the case of TsVF and TsCWE, antiserum to the crude extract of T. saginata larvae developed a total of 26 and 30 precipitates, respectively. Examination of the precipitation maps developed by the respective heterologous antiserum (vice-versa testing) showed that both TcVF and TsVF contained ten antigens sharing identity. For TcCWE and TsCWE, nearly the same number of shared antigens (20 and 18, respectively) could be demonstrated. For screening of IgG antibodies against T. saginata metacestodes from heavily and moderately infected calves (n = 6) by ELISA, VF and CWE antigens of both Taenia species were found to be potent reagents; TsVF was the most sensitive antigen.
Kalinna
B
B
Fachbereich Biologie, Philipps-Universität, Marburg, Federal Republic of Germany.
Becker
M
M
Geyer
E
E
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis*; D002417:Cattle; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007106:Immune Sera / Q000276:immunology; D005779:Immunodiffusion; D007123:Immunoelectrophoresis, Two-Dimensional; D008297:Male; D051379:Mice; D011233:Precipitin Tests; D013621:Taenia / Q000276:immunology*
D000947:Antigens, Helminth; D007106:Immune Sera