Amplification of both c-myc and c-raf-1 oncogenes in a human osteosarcoma.
Fourteen human bone and soft part tumor tissues were screened by Southern blot hybridization using five oncogene probes (c-myc, c-K-ras, c-fos, c-raf-1, and N-myc). Amplification of c-myc was found in two osteosarcomas and one malignant fibrous histiocytoma. One of these osteosarcomas had amplified c-raf-1 gene. Rearrangement of the amplified gene was not observed. This is the first report of c-raf-1 amplification in human cancer tissues.
Ikeda
S
S
Department of Biochemistry, Cancer Institute, Okayama.
Sumii
H
H
Akiyama
K
K
Watanabe
S
S
Ito
S
S
Inoue
H
H
Takechi
H
H
Tanabe
G
G
Oda
T
T
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D005260:Female; D005784:Gene Amplification; D006801:Humans; D008297:Male; D008875:Middle Aged; D012516:Osteosarcoma / Q000235:genetics*; D011519:Proto-Oncogenes