Monoclonal antibody against rat renal cell tumor-associated antigen as a new tool for the analysis of renal tumorigenesis.
A monoclonal antibody, NR-2 MAb, against one of the rat renal cell tumor-associated antigens was developed. NR-2 MAb belonged to IgM class and recognized a polypeptide of 81,000 daltons designated as NR-2 antigen, which is of non-glycoprotein nature with a pI of 4.6. NR-2 MAb was employed to probe the histogenesis of renal cell tumors in rats treated with N-ethyl-N-hydroxyethylnitrosamine followed by trisodium nitrilotriacetate monohydrate. Immunohistochemical analysis indicated that NR-2 antigen was expressed in simple hyperplasia, adenomatous hyperplasia and renal cell tumors. Both clear cells and basophilic cells of the simple hyperplasia showed equally strong positive reactions with NR-2 MAb, whereas the vacuolated epithelium was negative. Furthermore, the proximal tubules in nontumorous areas also expressed NR-2 antigen, suggesting that the hyperplastic lesions which eventually lead to renal cell tumors may derive from epithelia of proximal tubules and not directly from vacuolated epithelium. Such NR-2 antigen-positive epithelia of proximal tubules seem to be initiated cells. NR-2 MAb also cross-reacted with preneoplastic liver lesions.
Konishi
N
N
First Department of Pathology, Nara Medical College, Japan.
Kitahori
Y
Y
Shimoyama
T
T
Lin
J C
JC
Hiasa
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D002292:Carcinoma, Renal Cell / Q000032:analysis / Q000139:chemically induced / Q000175:diagnosis* / Q000473:pathology; D003505:Cyclodextrins; D006965:Hyperplasia; D007680:Kidney Neoplasms / Q000032:analysis / Q000139:chemically induced / Q000175:diagnosis* / Q000473:pathology; D008099:Liver / Q000473:pathology; D008297:Male; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000302:isolation & purification; D051381:Rats; D011919:Rats, Inbred Strains; D047392:beta-Cyclodextrins
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D003505:Cyclodextrins; D009363:Neoplasm Proteins; D047392:beta-Cyclodextrins; C031215:betadex