[Update on a titration method for measuring the interferon-producing capacity in humans].
To make it possible to measure the interferon-alpha (IFN-alpha) producing capacity in a great number of healthy donors and patients, we developed the simple method (the whole blood method). For the measurement of the IFN-producing capacity, the heparinized blood was incubated with Sendai virus at 37 degrees C for 20 hours. The IFN activity of the culture supernatants was determined by the cytopathic effect inhibition assay. We measured the IFN-producing capacity in 531 healthy donors and 130 cancer patients. The results showed that the IFN-producing capacity in cancer patients was significantly lower than that in healthy donors. Although there were individual variations in the IFN-producing capacity, no age and sex differences were observed. These results indicate that this method is useful for the measurement of IFN-producing capacity in human.
Kita
M
M
Département de Microbiologie, Université Médicale de la Préfecture de Kyoto, Japan.
Yamaji
M
M
Kishida
T
T
Imanishi
J
J
D000925:Anticoagulants / Q000494:pharmacology; D001780:Blood Coagulation Tests; D006801:Humans; D007372:Interferons / Q000097:blood*; D008214:Lymphocytes / Q000032:analysis; D009369:Neoplasms / Q000276:immunology; D014914:Whole Blood Coagulation Time
D000925:Anticoagulants; D007372:Interferons
Mise au point d'une méthode pour le titrage de la capacité de production d'interféron chez l'Homme.