MHC-restricted autoantigen-reactive T cell clones in multiple sclerosis.
Multiple sclerosis is a demyelinating disease of the central nervous system with genetic, viral and autoimmune characteristics. Myelin basic protein (MBP) is a suspected target autoantigen since it induces experimental autoimmune encephalomyelitis, an animal model closely resembling multiple sclerosis. The disease is mediated by Class II restricted, MBP-reactive T cells possessing the T helper/inducer phenotype. In the present study, we have isolated MBP-reactive T cell clones from the peripheral blood of a chronic progressive multiple sclerosis patient. The clones displayed blastogenic memory responses when rechallenged with the autoantigen and irradiated autologous lymphocytes. MBP recognition by the autoantigen-reactive T lymphocytes was restricted by major histocompatibility complex Class II antigens. Both CD4+8- and CD4-8+ MBP-reactive T cell clones were obtained.
Rohowsky-Kochan
C
C
University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark 07103.
Troiano
R
R
Cook
S D
SD
D000945:Antigens, Differentiation, T-Lymphocyte; D001324:Autoantigens; D015704:CD4 Antigens; D016827:CD8 Antigens; D002999:Clone Cells / Q000276:immunology; D006681:HLA-D Antigens; D006801:Humans; D007156:Immunologic Memory; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D009103:Multiple Sclerosis / Q000276:immunology*; D004676:Myelin Basic Protein / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000945:Antigens, Differentiation, T-Lymphocyte; D001324:Autoantigens; D015704:CD4 Antigens; D016827:CD8 Antigens; D006681:HLA-D Antigens; D004676:Myelin Basic Protein