Molecular mechanisms of avian neural crest cell migration on fibronectin and laminin.
We have examined the molecular interactions of avian neural crest cells with fibronectin and laminin in vitro during their initial migration from the neural tube. A 105-kDa proteolytic fragment of fibronectin encompassing the defined cell-binding domain (65 kDa) promoted migration of neural crest cells to the same extent as the intact molecule. Neural crest cell migration on both intact fibronectin and the 105-kDa fragment was reversibly inhibited by RGD-containing peptides. The 11.5-kDa fragment containing the RGDS cell attachment site was also able to support migration, whereas a 50-kDa fragment corresponding to the adjacent N-terminal portion of the defined cell-binding domain was unfavorable for neural crest cell movement. In addition to the putative "cell-binding domain," neural crest cells were able to migrate on a 31-kDa fragment corresponding to the C-terminal heparin-binding (II) region of fibronectin, and were inhibited in their migration by exogenous heparin, but not by RGDS peptides. Heparin potentiated the inhibitory effect of RGDS peptides on intact fibronectin, but not on the 105-kDa fragment. On substrates of purified laminin, the extent of avian neural crest cell migration was maximal at relatively low substrate concentrations and was reduced at higher concentrations. The efficiency of laminin as a migratory substrate was enhanced when the glycoprotein occurred complexed with nidogen. Moreover, coupling of the laminin-nidogen complex to collagen type IV or the low density heparan sulfate proteoglycan further increased cell dispersion, whereas isolated nidogen or the proteoglycan alone were unable to stimulate migration and collagen type IV was a significantly less efficient migratory substrate than laminin-nidogen. Neural crest cell migration on laminin-nidogen was not affected by RGDS nor by YIGSR-containing peptides, but was reduced by 35% after addition of heparin. The predominant motility-promoting activity of laminin was localized to the E8 domain, possessing heparin-binding activity distinct from that of the N-terminal E3 domain. Migration on the E8 fragment was reduced by greater than 70% after addition of heparin. The E1' fragment supported a minimal degree of migration that was RGD-sensitive and heparin-insensitive, whereas the primary heparin-binding E3 fragment and the cell-adhesive P1 fragment were entirely nonpermissive for cell movement.(ABSTRACT TRUNCATED AT 400 WORDS)
Perris
R
R
Developmental Biology Center, University of California, Irvine 92717.
Paulsson
M
M
Bronner-Fraser
M
M
D000818:Animals; D001665:Binding Sites; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D011508:Chondroitin Sulfate Proteoglycans / Q000494:pharmacology; D003094:Collagen / Q000378:metabolism; D005353:Fibronectins / Q000502:physiology*; D019812:Heparan Sulfate Proteoglycans; D006493:Heparin / Q000494:pharmacology; D006497:Heparan Sulfate / Q000494:pharmacology; D007797:Laminin / Q000502:physiology*; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000378:metabolism; D009432:Neural Crest / Q000166:cytology*; D009842:Oligopeptides / Q000494:pharmacology* / Q000502:physiology; D010446:Peptide Fragments / Q000502:physiology; D010978:Platelet Factor 4 / Q000502:physiology; D011784:Quail / Q000196:embryology*
D011508:Chondroitin Sulfate Proteoglycans; D003094:Collagen; D005353:Fibronectins; D019812:Heparan Sulfate Proteoglycans; D006493:Heparin; D006497:Heparan Sulfate; D007797:Laminin; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D009842:Oligopeptides; D010446:Peptide Fragments; D010978:Platelet Factor 4; C029967:nidogen; C061514:glycyl-tyrosyl-isoleucyl-glycyl-seryl-arginine; C047981:arginyl-glycyl-aspartic acid; C046617:arginyl-glycyl-aspartyl-serine