Stimulation of ornithine decarboxylase activity by arginine vasopressin in the rat medullary thick ascending limb of Henle's loop.
The effect of arginine vasopressin (AVP) on rat renal ornithine decarboxylase (ODC) activity was investigated by a cytochemical technique optimized for use in the medullary thick ascending limb of Henle's loop (mTAL). Stimulation of ODC activity by AVP was confined to the mTAL. Peaks in enzyme activity in cultured rat renal segments occurred after tissue had been exposed to AVP for 3 or 8 min and these times of maximal stimulation did not change with the concentration of AVP. There was a dose-dependent response in ODC activity over the AVP concentration range 0.01 10 fmol/l. The ODC response to AVP was totally blocked by specific antiserum to AVP and reduced by 70% with the specific inhibitor to ODC, difluoromethyl ornithine.
Charlton
J A
JA
Department of Medicine, The New Medical School, Newcastle upon Tyne.
Baylis
P H
PH
D000818:Animals; D001127:Arginine Vasopressin / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D000518:Eflornithine / Q000494:pharmacology; D005260:Female; D007106:Immune Sera / Q000494:pharmacology; D007679:Kidney Medulla / Q000187:drug effects* / Q000201:enzymology; D007684:Kidney Tubules / Q000187:drug effects*; D008138:Loop of Henle / Q000187:drug effects*; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D007106:Immune Sera; D001127:Arginine Vasopressin; D009955:Ornithine Decarboxylase; D000518:Eflornithine