title: Energy is required for maximal adherence of Neisseria gonorrhoeae to phagocytic and nonphagocytic cells.
abstract: The possibility that gonococcal energy might play a role in the interaction of Neisseria gonorrhoeae with both phagocytic and nonphagocytic cells was examined. Respiratory chain inhibitors including KCN and amobarbital resulted in reduction in gonococcal association with human neutrophils. Similar results were seen with HeLa cells and the human promyelocytic (HL-60) cell line. Identical conditions did not affect the opsonin-dependent association of Staphylococcus aureus with the same cell types. New protein synthesis by gonococci did not account for the observed reduction in association. These results suggest that energy is needed for maximal opsonin-independent association of gonococci with mammalian cells.
authors.affiliation: Department of Medicine, University of North Carolina, Chapel Hill 27599-7030.
authors.forename: R D
authors.initials: RD
authors.lastname: Weber
authors.forename: B E
authors.initials: BE
authors.lastname: Britigan
authors.forename: T
authors.initials: T
authors.lastname: Svendsen
authors.forename: M S
authors.initials: MS
authors.lastname: Cohen
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D001422:Bacterial Adhesion; D003486:Cyanides / Q000494:pharmacology; D004734:Energy Metabolism; D006801:Humans; D066298:In Vitro Techniques; D009344:Neisseria gonorrhoeae / Q000472:pathogenicity / Q000502:physiology*; D009504:Neutrophils / Q000382:microbiology; D010101:Oxygen Consumption / Q000187:drug effects; D010586:Phagocytes / Q000382:microbiology*; D010587:Phagocytosis; D014407:Tumor Cells, Cultured
chemical_list: D003486:Cyanides