A new method for studying human oocytes by light and electron microscopy.
Since ovarian follicles appear to be randomly oriented with respect to the plane of the section, the method of sectioning and examining follicles at their maximum diameter described here allows direct comparison between oocyte populations of women and small differences can be detected. Re-sectioning for EM allows selected follicles of interest to be examined at a higher resolution.
Wallace
B M
BM
Department of Biological Sciences, University of Birmingham, England.
Rollason
T P
TP
Sawers
R S
RS
D000328:Adult; D002467:Cell Nucleus / Q000648:ultrastructure; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008854:Microscopy, Electron; D008867:Microtomy / Q000379:methods; D009865:Oocytes / Q000648:ultrastructure*; D013194:Staining and Labeling