Saccharomyces cerevisiae chromatin associated protein Yp20 is a guanine nucleotide binding protein.
Yp20 is a 20kD protein whose role is still obscure which copurifies with yeast histones. Yeast histones were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred to nitrocellulose. Incubation of the nitrocellulose blots with [gamma-35S] GTP gamma S demonstrated that Yp20 is a GTP binding protein. A polyclonal antiserum raised against purified Yp20 cross reacted with bacterially expressed cHras and T24 Hras genuine ras products. The results obtained suggest that Yp20 is a yeast chromatin associated ras-related antigen.
Valansi
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Department of Biology, Technion-Israel Institute of Technology, Haifa.
Shalitin
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D000906:Antibodies; D002843:Chromatin / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000302:isolation & purification*; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism; D006657:Histones / Q000302:isolation & purification; D007124:Immunoenzyme Techniques; D008970:Molecular Weight; D010957:Plasmids; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000235:genetics; D016283:Proto-Oncogene Proteins p21(ras); D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013873:Thionucleotides / Q000378:metabolism
D000906:Antibodies; D002843:Chromatin; D005656:Fungal Proteins; D006657:Histones; D011518:Proto-Oncogene Proteins; D013873:Thionucleotides; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate; D019204:GTP-Binding Proteins; D016283:Proto-Oncogene Proteins p21(ras)