[OK 432-induced production of IFN-gamma in peripheral mononuclear cells from healthy subjects and patients with cancer].
OK 432, a well-established immunopotentiator, has been recently noticed as one of biological response modifiers (BRM). IFN-gamma, also called immune interferon, is regarded as an important immunoregulator secreted by T-lymphocytes. In the present study, we measured the in vitro production of IFN-gamma in human peripheral mononuclear cells (PMC) induced by OK 432. PMC were isolated from the peripheral blood with the Ficoll-Conray centrifugation technique. The number of cells for culture was adjusted to 1 x 10(6) cells/ml in RPMI-1640 medium supplemented with 10% fetal calf serum. Incubation was performed over 7 days at 37 degrees C in the presence of OK 432 at 0.17KE/ml in microculture plates. IFN-gamma secreted in the supernatants was measured consecutively during the observation period with radioimmunoassay. IFN-gamma production in PMC from healthy subjects was already detectable at 24 hours of culture and elevated gradually with incubation, reaching as much as 94.3 +/- 44.6 u/ml (mean +/- SD) after 7 days of culture. In contrast, the production of IFN-gamma in patients with cancer was severely suppressed as 21.9 +/- 25.4 u/ml after 7 days of culture (p less than 0.01). Furthermore, both surgical and radiation treatments inhibited the production of IFN-gamma in PMC from patients with cancer.
Matsumoto
H
H
Izutani
R
R
Terada
M
M
Noguchi
J
J
Shiraha
S
S
Kuyama
T
T
Aoki
N
N
D001688:Biological Products / Q000494:pharmacology*; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007369:Interferon Inducers; D007371:Interferon-gamma / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D009369:Neoplasms / Q000097:blood* / Q000628:therapy; D010844:Picibanil / Q000494:pharmacology*
D001688:Biological Products; D007369:Interferon Inducers; D010844:Picibanil; D007371:Interferon-gamma