Effect of striatal implantation of bovine adrenal chromaffin cells on turning behavior in a rat model of Parkinson's disease.
Rats lesioned unilaterally with 6-hydroxydopamine, as an animal model of Parkinson's disease, were tested for D-amphetamine-induced rotation. Buffer or isolated bovine adrenal chromaffin cells were implanted into the lesioned striatum. Rats were retested for rotation 1 and 4 weeks postimplantation. Those with surviving implants rotated significantly less than controls. HPLC assay of striata showed that implants contained elevated norepinephrine and epinephrine, but not dopamine. The results show that the bovine chromaffin cell implants can function as a restorative treatment for nigrostriatal damage, that neonatal implants work better than adult implants, and that such restoration occurs independent of dopamine levels.
Fitzgerald
L R
LR
Department of Pharmacology and Toxicology, Albany Medical College, NY 12208.
Glick
S D
SD
Schneider
A S
AS
D000311:Adrenal Glands / Q000166:cytology / Q000378:metabolism / Q000637:transplantation*; D000662:Amphetamines; D000818:Animals; D002417:Cattle; D002469:Cell Separation; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology*; D005260:Female; D006085:Graft Survival; D006892:Hydroxydopamines; D016627:Oxidopamine; D010300:Parkinson Disease / Q000378:metabolism / Q000503:physiopathology*; D051381:Rats; D013239:Stereotyped Behavior / Q000187:drug effects / Q000502:physiology*; D014183:Transplantation, Heterologous
D000662:Amphetamines; D006892:Hydroxydopamines; D016627:Oxidopamine