Autoimmune mice and stimulated normal mice make lambda 1 with increased V region diversity.
Previous studies of the genetic bases of murine SLE have defined gene segments that encode the H chain and the kappa L chain of anti-DNA, anti-Sm, and anti-IgG autoantibodies. As a result of these studies, the genetic origins of autoantibody H chains and kappa L chains are better understood, but little remains known about the genetic bases of autoantibody lambda-chains. Thus, we have analyzed serologically the germ-line and somatic origins of lambda 1 L chains in antibodies of normal mice and in both antibodies and autoantibodies of autoimmune mice. This study finds an increased lambda 1 diversity in both Ag-stimulated mice and autoimmune mice. This study also finds that the lambda 1 L chains in antibodies of unstimulated normal mice have the gene segment-encoded variable region, V lambda 1. In contrast, additional genetic processes appear to make the lambda 1 V regions of antibodies in Ag-stimulated normal mice and the lambda 1 V regions of both antibodies and autoantibodies in autoimmune mice. The increased lambda 1 diversity that we found in both Ag-stimulated mice and autoimmune mice might be caused by mutational processes creating antibody diversities. Therefore, the same somatic processes might be able to make both antibody and autoantibody lambda 1 diversities.
Slack
J H
JH
Indiana University School of Medicine, Department of Microbiology and Immunology, Indianapolis 46223.
Hang
L
L
Amundson
C
C
Clevinger
B L
BL
Dixon
F J
FJ
D000818:Animals; D000916:Antibody Diversity; D001323:Autoantibodies / Q000096:biosynthesis* / Q000235:genetics; D001327:Autoimmune Diseases / Q000235:genetics / Q000276:immunology*; D001667:Binding, Competitive; D007146:Immunoglobulin lambda-Chains / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008814:Mice, Inbred NZB; D011817:Rabbits; D011863:Radioimmunoassay; D013045:Species Specificity
D001323:Autoantibodies; D007146:Immunoglobulin lambda-Chains