[Effects of hematopoietic growth factor (GM-CSF: granulocyte-macrophage colony-stimulating factor) on thymocyte proliferation induced by interleukin-1 (IL-1)].
A semi-purified fraction obtained from P388 D1 cell line conditioned medium (P388 D1 CM) which contains Interleukin-1 (IL-1) and Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) stimulates murine thymocyte proliferation both in the absence and the presence of a suboptimal dose of phytohemagglutinin (PHA). Because this effect on thymocyte proliferation is always larger than that obtained with optimal concentrations of pure IL-1, we have investigated the possible involvement of GM-CSF in this semi-purified fraction mediated-thymocyte proliferation. We here show that the maximal level of thymocyte proliferation induced by the semi-purified fraction is comparable to that obtained by the co-addition of recombinant GM-CSF and IL-1. In addition, although GM-CSF alone induces no significant thymocyte proliferation, the presence of an anti-GM-CSF antiserum partially blocks the thymocyte proliferation induced by the semi-purified fraction. Thus, the capacity of the semi-purified fraction of P388 D1 to stimulate thymocyte proliferation appears to result from a synergistic action between GM-CSF and IL-1.
Herbelin
A
A
C.N.R.S. U.A. n. 122, I.N.S.E.R.M. U 25, Hôpital Necker, Paris.
Machavoine
F
F
Dy
M
M
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D003115:Colony-Stimulating Factors / Q000494:pharmacology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000494:pharmacology*; D007375:Interleukin-1 / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D008809:Mice, Inbred C3H; D010835:Phytohemagglutinins / Q000494:pharmacology; D013268:Stimulation, Chemical; D013601:T-Lymphocytes / Q000166:cytology*
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D007375:Interleukin-1; D010835:Phytohemagglutinins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor
Effet d'un facteur de croissance hématopoiétique (GM-CSF: granulocyte-macrophage colony-stimulating factor) sur la prolifération thymocytaire induite par l'interleukine-1 (IL-1).