title: Cell cycle related change of Ara-C transport in HL-60 cells after differentiation induction.
abstract: Using a promyelocytic leukemia cell line, HL-60, we studied the membrane transport of Ara-C before and after differentiation induced by retinoic acid (RA). In RA-treated cells, Ara-C transport was reduced and there was a concomitant increase of the ID50 values of Ara-C in comparison with the controls. By three different procedures to synchronize untreated cells, i.e. density arrest G1 phase enrichment, aphidicolin-induced S phase accumulation and the double isoleucine block method, we found that Ara-C transport was 30-50% higher in the S phase than in the G1 phase. Therefore, the observed decrease in Ara-C transport is, in part, due to the retarded growth accompanied by an accumulation of cells in the G1 phase after differentiation induction.
authors.affiliation: Department of Pediatrics, Kyoto University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Takimoto
authors.forename: M
authors.initials: M
authors.lastname: Kubota
authors.forename: S
authors.initials: S
authors.lastname: Tsuruta
authors.forename: T
authors.initials: T
authors.lastname: Kitoh
authors.forename: A
authors.initials: A
authors.lastname: Tanizawa
authors.forename: Y
authors.initials: Y
authors.lastname: Akiyama
authors.forename: H
authors.initials: H
authors.lastname: Mikawa
mesh_terms: D016590:Aphidicolin; D001692:Biological Transport; D002453:Cell Cycle; D002454:Cell Differentiation / Q000187:drug effects; D002462:Cell Membrane / Q000378:metabolism; D003561:Cytarabine / Q000378:metabolism*; D003842:Deoxycytidine Kinase / Q000235:genetics / Q000378:metabolism; D004224:Diterpenes / Q000494:pharmacology; D006801:Humans; D007399:Interphase / Q000187:drug effects; D015473:Leukemia, Promyelocytic, Acute; D009154:Mutation; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D004224:Diterpenes; D003561:Cytarabine; D016590:Aphidicolin; D014212:Tretinoin; D003842:Deoxycytidine Kinase