Genetic selection and DNA sequences of 4.5S RNA homologs.
A general strategy for cloning the functional homologs of an Escherichia coli gene was used to clone homologs of 4.5S RNA from other bacteria. The genes encoding these homologs were selected by their ability to complement a deletion of the gene for 4.5S RNA. DNA sequences of the regions encoding the homologs were determined. Since this approach does not require that the homologous genes hybridize with probes generated from the E. coli sequence, the sequences of the homologs were not all similar to the sequence of the E. coli gene. Despite the dissimilarity of the primary sequences of some of the homologs, all could be folded to obtain a similar structure.
Brown
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S
Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
Thon
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Tolentino
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E
D001412:Bacillus subtilis / Q000235:genetics*; D001419:Bacteria / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004275:DNA, Ribosomal / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D015698:Genomic Library; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012335:RNA, Ribosomal / Q000235:genetics*; D012641:Selection, Genetic; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D004275:DNA, Ribosomal; D012335:RNA, Ribosomal