Aluminium binding to chromatin DNA as revealed by formation of fluorescent complexes with 8-hydroxyquinoline and other ligands.
After aluminium mordanting of chicken blood smears, several chelating dyes and reagents were tested in order to detect the metal binding to chromatin DNA by fluorescence microscopy. 8-Hydroxyquinoline induced the most selective fluorescence reaction, which was abolished after DNase or trichloroacetic acid treatment. Morin, purpurin and nuclear fast red showed an intense nuclear fluorescence, but lower selectivity. Fluorescence was also observed in chromatin treated with morin or curcumin without mordanting. The simplicity and microscopical characteristics of the A1/8-hydroxyquinoline fluorescence reaction make it very suitable for cytological studies of chromatin.
Llorente
A R
AR
Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.
Del Castillo
P
P
Stockert
J C
JC
D000535:Aluminum / Q000378:metabolism*; D000818:Animals; D002614:Chelating Agents; D002645:Chickens; D002843:Chromatin / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D006912:Hydroxyquinolines; D008024:Ligands; D008856:Microscopy, Fluorescence; D015125:Oxyquinoline
D002614:Chelating Agents; D002843:Chromatin; D006912:Hydroxyquinolines; D008024:Ligands; D015125:Oxyquinoline; D004247:DNA; D000535:Aluminum