Restriction map of mycobacteriophage D29 and its deletion mutant F5.
A physical map of mycobacteriophage D29 was constructed, including positions for 25 restriction sites for 9 endunocleasic enzymes. D29 DNA contains about 48 150 bp. Analysis of a deletion mutant (F5) has allowed to determine the location of two non essential regions in the genome, allowing further insertion of foreign genes and construction of cosmids.
Lazraq
R
R
Unité de Tuberculose et Mycobactéries, Paris, France.
Moniz-Pereira
J
J
Clavel-Sérès
S
S
Clément
F
F
David
H L
HL
D002872:Chromosome Deletion; D003360:Cosmids; D004279:DNA, Viral / Q000235:genetics; D005822:Genetic Vectors; D009154:Mutation; D009160:Mycobacteriophages / Q000235:genetics*; D009161:Mycobacterium / Q000235:genetics*; D015183:Restriction Mapping; D014764:Viral Proteins / Q000235:genetics / Q000302:isolation & purification; D015678:Viral Structural Proteins
D004279:DNA, Viral; D014764:Viral Proteins; D015678:Viral Structural Proteins