Determination of the absolute concentrations of monoamine oxidase A and B in human tissues.
The concentrations of monoamine oxidase-A and -B were determined in homogenates of human cerebral cortex, caudatus and placenta and in human platelet-rich plasma and platelet membranes by determining the specific binding of tritium-labelled pargyline. The concentrations of the two enzyme forms were similar in both human brain regions examined. Determinations of the minimum quantities of clorgyline, (-)-deprenyl, J-508 or pargyline necessary to give complete inhibition of enzyme activity was found to give overestimates of the amounts of monoamine oxidase-A and -B present due to nonspecific binding of these inhibitors.
O'Carroll
A M
AM
Department of Biochemistry, Trinity College, Dublin, Ireland.
Anderson
M C
MC
Tobbia
I
I
Phillips
J P
JP
Tipton
K F
KF
D001344:Autopsy; D001665:Binding Sites / Q000187:drug effects; D001792:Blood Platelets / Q000201:enzymology*; D001921:Brain / Q000201:enzymology*; D003010:Clorgyline / Q000494:pharmacology; D006801:Humans; D008995:Monoamine Oxidase / Q000032:analysis*; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology; D010293:Pargyline / Q000494:pharmacology; D010920:Placenta / Q000201:enzymology*; D012642:Selegiline / Q000494:pharmacology
D008996:Monoamine Oxidase Inhibitors; D012642:Selegiline; D010293:Pargyline; D008995:Monoamine Oxidase; D003010:Clorgyline