Immunomodulation of polymorphonuclear leukocytes by D53 Immucytal and its constitutive fractions.
D53 (Immucytal) is a compositive vaccine made of immunogenic ribosomes extracted from 4 bacterial species (Klebsiella pneumoniae, Haemophilus influenzae, Streptococcus pyogenes and Streptococcus pneumoniae) associated with a membrane proteoglycan from a non-encapsulated strain of Klebsiella pneumoniae (MPG-Kp). In this work we have studied the effect of the compound on human polymorphonuclear leukocyte (PMN) function "in vitro". We have demonstrated that D53 was able to significantly increase Fc- receptor dependent phagocytosis without modify the C3-receptor dependent activity. Furthermore D53 enhanced the oxidative metabolism (evaluated by chemiluminescence) both using cells in resting conditions or after stimulation with phagocytable or soluble stimuli. On the contrary D53 caused a dose-dependent inhibition of PMN migration toward different chemoattractants. Using the two constitutive fractions of the compound (ribosomes and proteoglycans) we have observed that the MPG-Kp component was mainly responsible for the modulating activity of the drug on human PMNs.
Minonzio
F
F
Istituto di Clinica Medica I, University of Milan.
Ongari
A M
AM
Venegoni
E
E
Carbonelli
V
V
Licciardello
L
L
Capsoni
F
F
D000276:Adjuvants, Immunologic; D001428:Bacterial Vaccines / Q000494:pharmacology*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D018073:Haemophilus Vaccines; D006801:Humans; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology*; D010587:Phagocytosis / Q000187:drug effects; D022242:Pneumococcal Vaccines; D022541:Streptococcal Vaccines
D000276:Adjuvants, Immunologic; D001428:Bacterial Vaccines; D018073:Haemophilus Vaccines; D022242:Pneumococcal Vaccines; D022541:Streptococcal Vaccines; C024220:D 53