title: Immunomagnetic T-lymphocyte depletion (ITLD) of rat bone marrow using OX-19 monoclonal antibody.
abstract: Graft versus host disease (GVHD) may be abrogated and host survival prolonged by in vitro depletion of T lymphocytes from bone marrow (BM) prior to allotransplantation. Using a mouse anti-rat pan T-lymphocyte monoclonal antibody (OX19) bound to monosized, magnetic, polymer beads, T lymphocytes were removed in vitro from normal bone marrow. The removal of the T lymphocytes was confirmed by flow cytometry. Injection of the T-lymphocyte-depleted bone marrow into fully allogeneic rats prevents the induction of GVHD and prolongs host survival.
authors.affiliation: Department of Surgery, University of Pittsburgh School of Medicine, PA 15261.
authors.forename: A L
authors.initials: AL
authors.lastname: Hoffman
authors.forename: L
authors.initials: L
authors.lastname: Makowka
authors.forename: D V
authors.initials: DV
authors.lastname: Cramer
authors.forename: X
authors.initials: X
authors.lastname: Cai
authors.forename: A
authors.initials: A
authors.lastname: Pascualone
authors.forename: B F
authors.initials: BF
authors.lastname: Banner
authors.forename: D G
authors.initials: DG
authors.lastname: Sheahan
authors.forename: P
authors.initials: P
authors.lastname: Rettinger
authors.forename: T E
authors.initials: TE
authors.lastname: Starzl
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation / Q000276:immunology*; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D005434:Flow Cytometry; D006086:Graft vs Host Disease / Q000517:prevention & control; D008212:Lymphocyte Depletion; D008280:Magnetics; D051381:Rats; D011917:Rats, Inbred Lew / Q000276:immunology; D011919:Rats, Inbred Strains / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal