31P-NMR spectroscopy of phosphate compartmentation during ischaemia in hearts protected by cardioplegic treatment.
Four tissue compartments, differing in proton and inorganic phosphate concentration, were resolved by 31P-NMR spectroscopy in samples from dog hearts after cardioplegic treatment with HTK solution. Inversion of the physiological cytoplasmic-mitochondrial pH gradient was observed. The considerable ensuing acidosis of the matrix is discussed with regard to a possible delocalization of ferrous ions.
Stier
A
A
Max-Planck-Institut für Biophysikalische Chemie, Göttingen, W. Germany.
Finch
S A
SA
Schäfer
H
H
Gebhardt
M M
MM
Bretschneider
H J
HJ
D000818:Animals; D002314:Cardioplegic Solutions / Q000494:pharmacology*; D003327:Coronary Disease / Q000378:metabolism*; D004285:Dogs; D005947:Glucose / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D008353:Mannitol / Q000494:pharmacology; D009206:Myocardium / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism*; D011189:Potassium Chloride / Q000494:pharmacology; D011343:Procaine / Q000494:pharmacology
C039984:Bretschneider cardioplegic solution; D002314:Cardioplegic Solutions; D010710:Phosphates; D008353:Mannitol; D011343:Procaine; D011189:Potassium Chloride; D005947:Glucose