Identification of a protein that binds specifically to RNA from the first exon of c-myc.
A 55 kilodalton protein present in HeLa and MEL cells, which binds specifically to RNA derived from the first exon of the human c-myc gene, has been identified using a UV-induced crosslinking assay. This protein, called p55, is found in both cytoplasmic and nuclear fractions. The binding site on the RNA, defined using deletion analysis and synthetic oligoribonucleotides, is a purine rich region located between the two major transcriptional initiation sites P1 and P2. Possible involvement of p55 in a number of regulatory events is discussed.
Parkin
N T
NT
Department of Biochemistry, McGill University, Montreal, Quebec, Canada.
Sonenberg
N
N
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002352:Carrier Proteins / Q000032:analysis*; D005091:Exons; D006801:Humans; D051379:Mice; D011519:Proto-Oncogenes; D016601:RNA-Binding Proteins; D014158:Transcription, Genetic
D002352:Carrier Proteins; D016601:RNA-Binding Proteins