Decay time distribution analysis of Yt-base in benzene-methanol mixtures.
Frequency-domain fluorometry was used to measure intensity decays of synthetic Yt-base in mixtures of benzene-methanol at 20 degrees C. Multiexponential analysis shows that the decay of Yt-base fluorescence in benzene and methanol can be well fitted to a single-exponential model with tau = 9.67 ns and 6.25 ns respectively. In mixtures of benzene-methanol the decays became heterogeneous, and the maximum of heterogeneity observed was in a mixture containing 6% methanol. Since we expected a distribution of Yt-base solvation states in the solvent mixtures, and because the decay times of Yt-base are sensitive to solvent, we analyzed the data in terms of decay time distributions. The goodness-of-fit for the unimodal distribution model which has two floating parameters was equivalent to that found using the double exponential model with three floating parameters. The Lorentzian distribution model appears to provide a slightly superior fit relative to the Gaussian distribution model. These results suggest that the intensity decays of solvent-sensitive fluorophores in mixed solvents are described by a distribution of decay times.
Gryczynski
I
I
University of Maryland, School of Medicine, Department of Biological Chemistry, Baltimore 21201.
Wiczk
W
W
Lakowicz
J R
JR
Johnson
M L
ML
D001554:Benzene; D005456:Fluorescent Dyes; D006147:Guanine / Q000031:analogs & derivatives*; D000432:Methanol; D015233:Models, Statistical; D010777:Photochemistry; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; C012099:wye; D006147:Guanine; D001554:Benzene; D000432:Methanol