Changes in the SDS elutability of fibrinogen adsorbed from plasma to polymers.
Baboon fibrinogen was adsorbed from diluted plasma solutions to glass, polyethylene, polystyrene, polydimethylsiloxane, poly(ethylene)terephthalate, and Biomer. Following adsorption, half of the samples were immediately placed in sodium dodecyl sulfate (SDS) solutions to elute the protein, while the others were stored in buffer for up to 5 days and then eluted in SDS. The elution was typically incomplete, but depended on the plasma concentration and the residence time. The elutability was generally lower for fibrinogen adsorbed from more diluted plasma, and substantially lower for samples on which the fibrinogen had resided for 5 days. The non-elutable portion of the protein layer formed rapidly on polystyrene, while on polyethylene elutability was high initially, followed by a gradual decrease.
Rapoza
R J
RJ
Department of Chemical Engineering, University of Washington, Seattle 98195.
Horbett
T A
TA
D000327:Adsorption; D002021:Buffers; D005340:Fibrinogen / Q000737:chemistry* / Q000302:isolation & purification; D010949:Plasma; D011108:Polymers; D012967:Sodium Dodecyl Sulfate; D013997:Time Factors
D002021:Buffers; D011108:Polymers; D012967:Sodium Dodecyl Sulfate; D005340:Fibrinogen