Effect of nalidixic acid on DNA repair in rat hepatocytes.
Nalidixic acid, a DNA topoisomerase inhibitor, has been reported to inhibit DNA repair in some mammalian systems. To investigate the effect of nalidixic acid on DNA repair in cultured rat hepatocytes, DNA damage was induced by ultraviolet light or N-methyl-N-nitro-N'-nitrosoguanidine. The presence of aphidicolin, a DNA polymerase alpha inhibitor resulted in a decrease in DNA repair. Nalidixic acid had no inhibitory effect. Neither aphidicolin nor nalidixic acid induced DNA repair. These results indicate that nalidixic acid does not damage DNA or inhibit DNA repair processes in hepatocytes.
McQueen
C A
CA
American Health Foundation, Valhalla, NY.
Rosado
R R
RR
Williams
G M
GM
D000818:Animals; D016590:Aphidicolin; D002478:Cells, Cultured; D004257:DNA Polymerase II / Q000037:antagonists & inhibitors; D004260:DNA Repair / Q000187:drug effects*; D004224:Diterpenes / Q000494:pharmacology; D008099:Liver / Q000166:cytology; D008297:Male; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D009268:Nalidixic Acid / Q000494:pharmacology*; D051381:Rats; D011916:Rats, Inbred F344; D014466:Ultraviolet Rays
D004224:Diterpenes; D008769:Methylnitronitrosoguanidine; D016590:Aphidicolin; D009268:Nalidixic Acid; D004257:DNA Polymerase II