The gene for the precursor of plastocyanin from the cyanobacterium Anabaena sp. PCC 7937: isolation, sequence and regulation.
The gene encoding plastocyanin (petE1) from Anabaena sp. PCC 7937 was isolated using two sets of mixed oligonucleotide hybridization probes derived from conserved regions in the protein. Plastocyanin is encoded as a preprotein of 139 amino acids. The amino-terminal extension of 34 residues has all the characteristics of a signal peptide and is probably involved in translocation of preplastocyanin over the thylakoid membrane. The level of the petE1 mRNA, a single transcript of about 740 bases, was found to be severely reduced under conditions of Cu2+ deficiency. The petE1 gene was transferred to the genome of Synechococcus sp. PCC 7942, which did not appear to contain a structural gene for plastocyanin itself. The integrated gene becomes expressed at the transcriptional level, regardless of the amount of Cu2+ available.
Van der Plas
J
J
Department of Molecular Cell Biology, University of Utrecht, The Netherlands.
Bovy
A
A
Kruyt
F
F
de Vrieze
G
G
Dassen
E
E
Klein
B
B
Weisbeek
P
P
D000595:Amino Acid Sequence; D001483:Base Sequence; D001692:Biological Transport; D003001:Cloning, Molecular; D003300:Copper / Q000502:physiology; D000458:Cyanobacteria / Q000235:genetics* / Q000302:isolation & purification; D004247:DNA / Q000235:genetics / Q000378:metabolism; D015342:DNA Probes; D005786:Gene Expression Regulation; D005796:Genes; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D010970:Plastocyanin / Q000096:biosynthesis / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics*; D021382:Protein Sorting Signals / Q000235:genetics; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014170:Transformation, Genetic
D015342:DNA Probes; D010940:Plant Proteins; D011498:Protein Precursors; D021382:Protein Sorting Signals; D003300:Copper; D004247:DNA; D010970:Plastocyanin