Reversed-phase liquid chromatographic analysis of o-phthaldialdehyde-derivatized free amino-acids in two types of goldfish muscles.
o-Phthaldialdehyde-derivatized free amino-acids from muscle tissues of goldfish (Carassius auratus L.) were determined using reversed-phase liquid chromatography (3-microns Ultrasphere ODS) with gradient elution and UV detection of the analyte. An off-line, pre-column derivatization procedure was applied. A full analysis of the derivatives was completed within 40 min. The method showed good selectivity, sensitivity and reproducibility. The developed procedure was employed to quantify free amino-acids in white and red muscle of non-fasted, normoxic goldfish. The differences in the amounts of the individual amino-acids in white and red muscle are given. The rôle of two of the quantitatively most important free amino-acids in white and red muscle of goldfish, histidine and taurine is briefly discussed.
van der Boon
J
J
Gorlaeus Laboratories, Department of Animal Physiology, Leiden, The Netherlands.
van den Thillart
G E
GE
Addink
A D
AD
D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D002853:Chromatography, Liquid; D006054:Goldfish / Q000378:metabolism*; D009132:Muscles / Q000737:chemistry*; D015203:Reproducibility of Results; D009764:o-Phthalaldehyde
D000596:Amino Acids; D009764:o-Phthalaldehyde