Purification, cyanogen bromide cleavage, and amino terminus sequencing of class 1 and class 3 outer membrane proteins of meningococci.
Meningococcal class 1 and 3 outer membrane proteins (OMPs) were subjected to cyanogen bromide treatment. The class 3 OMP was found to be resistant to cyanogen bromide, while the class 1 OMP was cut into two main fragments of 25 and 17 kilodaltons. The N-terminal sequences were determined for class 1 and class 3 proteins, which exhibit similarities to one another and to OMP I of gonococci. The C-terminal class 1 OMP fragment bound the bactericidal monoclonal antibodies tested.
Poolman
J T
JT
Department of Bacterial Vaccine Development, National Institute for Public Health and Environmental Protection (RIVM), Bilthoven, The Netherlands.
Timmermans
H A
HA
Teerlink
T
T
Seid
R C
RC
D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D015153:Blotting, Western; D003488:Cyanogen Bromide; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009345:Neisseria meningitidis / Q000032:analysis*; D010446:Peptide Fragments / Q000032:analysis
D001425:Bacterial Outer Membrane Proteins; D010446:Peptide Fragments; D003488:Cyanogen Bromide