Effects of adrenalectomy and acute replacement by corticosteroids on distal acidification.
The role of adrenocortical steroids in distal nephron acidification was studied in rats by measuring urine minus blood PCO2 differences (U-B PCO2) in control, sham-operated, and adrenalectomized (ADX) animals. Operations were performed 48 h before experiments. During the experiments, all rats received an infusion of 0.35-0.60 M NaHCO3, leading to urine bicarbonate concentrations in the order of 100-200 mM. Adrenalectomized rats had significantly decreased U-B PCO2 (11.9 +/- 1.99 mmHg; 1 mmHg = 133.3 Pa) with respect to sham-operated rats (39.9 +/- 1.26 mmHg). In another series, ADX rats received supplements of the adrenal steroids corticosterone, aldosterone, and 18-hydroxycorticosterone 100 min before the experiment. U-B PCO2 increased after hormone administration: corticosterone, 30.0 +/- 2.13 mmHg; aldosterone, 26.6 +/- 1.74 mmHg; 18-hydroxycorticosterone, 29.0 +/- 1.60 mmHg; but none restored these values to normal. Combinations of two hormones were also used; only aldosterone + corticosterone restored U-B PCO2 to normal: 39.0 +/- 1.66 mmHg. Renal phosphate excretion (but not urine phosphate levels) decreased significantly in ADX as compared with sham-operated rats. Extracellular volume was not significantly affected in ADX rats, which received ad libitum 0.9% NaCl for drinking. It is concluded that distal tubular acidification, as evaluated by U-B PCO2, is dependent on cortical steroids.
Damasco
M C
MC
Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina.
Ansaldo
M
M
Malnic
G
G
D015069:18-Hydroxycorticosterone / Q000494:pharmacology; D000305:Adrenal Cortex Hormones / Q000494:pharmacology*; D000315:Adrenalectomy; D000450:Aldosterone / Q000494:pharmacology; D000818:Animals; D001639:Bicarbonates / Q000652:urine; D002245:Carbon Dioxide / Q000378:metabolism; D003345:Corticosterone / Q000494:pharmacology; D005110:Extracellular Space / Q000378:metabolism; D005919:Glomerular Filtration Rate / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D000305:Adrenal Cortex Hormones; D001639:Bicarbonates; D002245:Carbon Dioxide; D000450:Aldosterone; D015069:18-Hydroxycorticosterone; D003345:Corticosterone