An enhanced chemiluminescent enzyme immunoassay for follicle stimulating hormone.
An enhanced chemiluminescent enzyme immunoassay for serum follicle stimulating hormone is described which involves sequential reaction of anti-follicle stimulating hormone antibody immobilised to the inside surface of an opaque microtitre plate with sample, monoclonal anti-alpha thyroid stimulating hormone antibody, and an anti-mouse IgG - horseradish peroxidase conjungate. Bound peroxidase activity was measured using a p-hydroxycinnamic acid enhanced chemiluminescent luminol-hydrogen peroxide reaction. The assay was sensitive (detection limit 0.01 mU/well) precise (intra-assay precision 2.5-8.1%, inter-assay precision 6.7-11.9%) and results obtained with this assay and a competitive radioimmunoassay were in good agreement (correlation coefficient 0.98).
Sankolli
G M
GM
Department of Clinical Chemistry, Wolfson Research Laboratories, Queen Elizabeth Medical Centre, Birmingham, UK.
Lynch
S S
SS
Rudd
B T
BT
Thorpe
G H
GH
Stott
R A
RA
Kricka
L J
LJ
D006063:Chorionic Gonadotropin / Q000032:analysis; D003429:Cross Reactions; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D005640:Follicle Stimulating Hormone / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D008163:Luminescent Measurements; D007986:Luteinizing Hormone / Q000032:analysis; D011388:Prolactin / Q000032:analysis; D011863:Radioimmunoassay; D013972:Thyrotropin / Q000032:analysis
D006063:Chorionic Gonadotropin; D011388:Prolactin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D013972:Thyrotropin