A new bacteriocinogenic activity: megacin BII encoded by plasmid pSE 203 in strains of Bacillus megaterium.
Mesophilic strains producing a new bacteriocin: Megacin BII, have been isolated from strains of Bacillus megaterium. Facultatively thermophilic strains producing Megacin BI were less sensitive to this new activity than non-producing mesophiles and strains producing Megacin BII were also more resistant to Megacin BI. Strains producing Megacin BII contained a large plasmid of 36.10(6):pSE 203. This plasmid was introduced into non-megacinogenic acceptor strains by protoplast transformation, they then became megacin producers and immune to Megacin BII. Plasmid pSE 203 has been mapped with endonucleases. No similarity to the Megacin A plasmids pBM 309 [Rostás et al. (1980) and pBM 113 (von Tersch and Carlton (1983 b)] was evident.
Ståhl
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Department of Microbiology, University of Lund, Sweden.
D001345:Autoradiography; D001410:Bacillus megaterium / Q000235:genetics* / Q000378:metabolism; D001430:Bacteriocins / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D008530:Megacins / Q000096:biosynthesis / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D015183:Restriction Mapping; D014169:Transformation, Bacterial
D001430:Bacteriocins; D004269:DNA, Bacterial; D008530:Megacins