Effects of selected carbohydrates and the contribution of the prophenoloxidase cascade system to the adhesion of strains of Pseudomonas aeruginosa and Proteus mirabilis to hemocytes of nonimmune larval Galleria mellonella.
The adhesion of strains of Pseudomonas aeruginosa and Proteus mirabilis to the plasmatocytes and granular cells of nonimmune larval Galleria mellonella was influenced by and varied with the type of carbohydrate. Laminarin enhanced prophenoloxidase activation and bacterial adhesion to the hemocytes whereas sucrose suppressed both activities. For all other sugars there was no correlation between bacterial adhesion to the hemocytes and phenoloxidase activity. It is proposed that bacterial adhesion to the hemocytes may be mediated by both lectinlike binding and components of the prophenoloxidase activating system acting like opsonins.
Dunphy
G B
GB
Department of Microbiology and Immunology, Queen's University, Kingston, Ont., Canada.
Chadwick
J S
JS
D000818:Animals; D001422:Bacterial Adhesion; D001773:Blood Cells / Q000382:microbiology*; D050260:Carbohydrate Metabolism; D004156:Catechol Oxidase / Q000378:metabolism; D004792:Enzyme Precursors / Q000378:metabolism; D006434:Hemocytes / Q000382:microbiology*; D007814:Larva; D007915:Lepidoptera; D009895:Opsonin Proteins / Q000378:metabolism; D011513:Proteus mirabilis / Q000502:physiology*; D011550:Pseudomonas aeruginosa / Q000502:physiology*
D004792:Enzyme Precursors; D009895:Opsonin Proteins; C038451:pro-phenoloxidase; D004156:Catechol Oxidase