Modifying effects of components of plant essence on the induction of sister-chromatid exchanges in cultured Chinese hamster ovary cells.
The influence of 21 kinds of components of plant essence on sister-chromatid exchanges (SCEs) induced by mitomycin C was investigated in cultured Chinese hamster CHO K-1 cells. Posttreatment with scopoletin, jasmone, caffeic acid and ferulic acid significantly increased the frequency of SCEs. Further investigation of the SCE-enhancing effect of analogues of caffeic acid and ferulic acid revealed that an alpha,beta-unsaturated carbonyl group may be necessary for SCE-enhancing effects. The influence of caffeic acid and ferulic acid on X-ray- or UV-induced SCEs was also studied. The frequencies of SCEs induced by UV were increased by treatment with these compounds. This increasing effect was observed in the S phase of the cell cycle. On the contrary, X-ray-induced SCEs were reduced by the treatment with these compounds. The decreasing effect was observed in the G1 phase but not in the S or G2 phase. To explain these contradictory results, we assumed that caffeic acid and ferulic acid may modify the repair of DNA strand breaks.
Sasaki
Y F
YF
Institute of Environmental Toxicology, Tokyo, Japan.
Imanishi
H
H
Ohta
T
T
Shirasu
Y
Y
D000818:Animals; D002109:Caffeic Acids / Q000633:toxicity; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D003373:Coumaric Acids / Q000633:toxicity; D006224:Cricetinae; D003412:Cricetulus; D004357:Drug Synergism; D066298:In Vitro Techniques; D016685:Mitomycin; D008937:Mitomycins / Q000633:toxicity; D010936:Plant Extracts / Q000633:toxicity*; D012854:Sister Chromatid Exchange / Q000187:drug effects* / Q000528:radiation effects; D013329:Structure-Activity Relationship; D014466:Ultraviolet Rays; D014965:X-Rays
D002109:Caffeic Acids; D003373:Coumaric Acids; D008937:Mitomycins; D010936:Plant Extracts; D016685:Mitomycin; C004999:ferulic acid; C040048:caffeic acid