Comparative evaluation of a latex test for the identification of Staphylococcus aureus.
A rapid latex agglutination test, Staphaurex, was tested for its ability to identify Staphylococcus aureus using 72 reference strains and 785 clinical isolates of the family Micrococcaceae. All reference strains of Staphylococcus aureus were Staphaurex-positive. Non-Staphylococcus aureus reference strains were negative. Using clinical strains, the results of the Staphaurex test were compared with the results of other tests commonly used to identify Staphylococcus aureus. A total of 393 clinical isolates were classified as Staphylococcus aureus. The Staphaurex, slide coagulase, tube coagulase/human plasma and tube coagulase/rabbit plasma tests correctly identified 98%, 93.6%, 93.6% and 97.5% of the Staphylococcus aureus strains, respectively. The performance of the Staphaurex test, in terms of sensitivity and specificity, was significantly better than the slide coagulase test. It was as sensitive and almost as specific as the tube coagulase rabbit test and more sensitive than the tube coagulase human test.
Stevens
M
M
Public Health Laboratory, Royal Infirmary, Leicester, UK.
Geary
C
C
D005069:Evaluation Studies as Topic; D006801:Humans; D007841:Latex Fixation Tests / Q000379:methods*; D013211:Staphylococcus aureus / Q000302:isolation & purification*