Restricted immunoglobulin variable region gene usage by normal Ly-1 (CD5+) B cells that recognize phosphatidyl choline.
5-15% of lymphocytes in the peritoneums of normal adult B10.H-2aH-4bp/Wts (2a4b) mice are CD5+ (Ly-1) B cells that recognize phosphatidyl choline (PtC), a phospholipid component of all mammalian cells. We produced a set of IgM-secreting hybridomas from the peritoneal cells of normal, adult 2a4b mice. We found that this set of hybridomas shows a similarly high frequency of antibodies specific for PtC (21 of 86) that also react with bromelain-treated mouse erythrocytes. Restriction fragment analysis of Ig gene rearrangements and analysis of expressed Ig idiotypes reveal that these cells use a restricted set of variable region genes to generate the PtC-specific antibodies. The Ig genes used by the PtC-specific hybridomas appear to be the same as those found in the PtC-specific Ly-1 B cell lymphomas, CH27 and CH34.
Mercolino
T J
TJ
Department of Microbiology and Immunology, University of North Carolina, Chapel Hill 27599.
Locke
A L
AL
Afshari
A
A
Sasser
D
D
Travis
W W
WW
Arnold
L W
LW
Haughton
G
G
D000818:Animals; D000918:Antibody Specificity; D000950:Antigens, Ly; D001402:B-Lymphocytes / Q000032:analysis / Q000145:classification* / Q000276:immunology; D001666:Binding Sites, Antibody; D001963:Bromelains; D004912:Erythrocytes / Q000276:immunology; D005803:Genes, Immunoglobulin; D006825:Hybridomas / Q000145:classification / Q000378:metabolism; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007130:Immunoglobulin Idiotypes / Q000032:analysis; D007147:Immunoglobulin Light Chains / Q000235:genetics; D007075:Immunoglobulin M / Q000276:immunology; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D051379:Mice; D010641:Phenotype; D010713:Phosphatidylcholines / Q000276:immunology*
D000950:Antigens, Ly; D007143:Immunoglobulin Heavy Chains; D007130:Immunoglobulin Idiotypes; D007147:Immunoglobulin Light Chains; D007075:Immunoglobulin M; D007135:Immunoglobulin Variable Region; D010713:Phosphatidylcholines; D001963:Bromelains