A collaborative study of the analysis of doxycycline hyclate by high-performance liquid chromatography on polystyrene-divinylbenzene packing materials.
An improved method for the analysis of doxycycline hyclate by high-performance liquid chromatography using polystyrene-divinylbenzene column packings is described. The separation obtained with this method was compared with that of other, recently described methods. The improved method was examined in a collaborative study involving five separate laboratories, using 11 different columns and four discrete samples. The main component and impurities were determined. An analysis of variance showed absence of consistent laboratory bias and presence of significant laboratory-sample interaction. Estimates for the repeatability and reproducibility of the method, expressed as relative standard deviations (RSD) of the result of the determination of doxycycline, were found to be 0.9 and 1.2%, respectively.
Hoogmartens
J
J
Katholieke Universiteit Leuven, Laboratorium voor Farmaceutische Chemie, Belgium.
Khan
N H
NH
Vanderhaeghe
H
H
van der Leeden
A L
AL
Oosterbaan
M
M
Veld-Tulp
G L
GL
Plugge
W
W
van der Vlies
C
C
Mialanne
D
D
Melamed
R
R
D002851:Chromatography, High Pressure Liquid; D004318:Doxycycline / Q000031:analogs & derivatives* / Q000032:analysis; D011137:Polystyrenes; D014753:Vinyl Compounds
D011137:Polystyrenes; D014753:Vinyl Compounds; C004985:divinyl benzene; D004318:Doxycycline