Detection of HTLV-I genome in seronegative infants born to HTLV-I seropositive mothers by polymerase chain reaction.
We applied the polymerase chain reaction (PCR) method to detect gag, env and pX sequences of human T cell leukemia virus type I (HTLV-I) provirus in peripheral blood lymphocytes of seronegative infants born to HTLV-I seropositive mothers. Out of 22, five subjects were found to contain the HTLV-I provirus genome. Two of the five cases were judged to be negative for not only anti-HTLV-I antibodies but also the viral antigens on cultivated lymphocytes by the conventional antibody/antigen detection methods. These results indicate that PCR is of great use as a simple and highly sensitive method detect HTLV-I infection.
Saito
S
S
Department of Obstetrics and Gynecology, Nara Medical University.
Ando
Y
Y
Furuki
K
K
Kakimoto
K
K
Tanigawa
T
T
Moriyama
I
I
Ichijo
M
M
Nakamura
M
M
Ohtani
K
K
Sugamura
K
K
D000328:Adult; D001942:Breast Feeding; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D015490:HTLV-I Infections / Q000382:microbiology* / Q000635:transmission; D015368:Human T-lymphotropic virus 1 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007231:Infant, Newborn; D008214:Lymphocytes / Q000382:microbiology; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000382:microbiology*; D011533:Proviruses / Q000235:genetics / Q000302:isolation & purification*; D012698:Serologic Tests
D004279:DNA, Viral