An evolutionarily conserved protein fraction stably linked to DNA.
Chromatins from four evolutionarily remote species (insect, fish, amphibian and bird) were isolated, high-salt-extracted and extensively deproteinized to remove noncovalently associated proteins. A protein fraction resisting the extraction procedures was found firmly linked to DNA in all four chromatins. Two-dimensional tryptic peptide mapping revealed a remarkable evolutionary conservativeness of this protein component, suggesting an indispensable function for it in the nucleus.
Avramova
Z
Z
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.
Mikhailov
I
I
Tsanev
R
R
D000818:Animals; D005075:Biological Evolution; D002347:Carps / Q000235:genetics; D002645:Chickens / Q000235:genetics; D002843:Chromatin / Q000032:analysis*; D004268:DNA-Binding Proteins / Q000032:analysis*; D004331:Drosophila melanogaster / Q000235:genetics; D009687:Nuclear Proteins / Q000032:analysis*; D010449:Peptide Mapping; D011895:Rana ridibunda / Q000235:genetics
D002843:Chromatin; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins