Evaluation of a method for rapid detection of penicillinase-producing Neisseria gonorrhoeae in urethral exudates.
A substrate, pyridine-2-azo-p-dimethylaniline cephalosporin (PADAC; Diagnostic Pasteur, Marnes-La-Coquette, France), for detection of penicillinase-producing Neisseria gonorrhoeae (PPNG) on isolated colonies grown on agar was compared with the nitrocefin reference test (Cefinase; Biomerieux, Marcy l'Etoile, France). The PADAC test was also used to detect PPNG directly on urethral exudates. All 38 PPNG-containing and 29 of 30 PPNG-negative urethral pus isolates gave results in agreement with those obtained on cultured isolates. We conclude that direct detection of PPNG in urethral exudates from males is simple, rapid, reliable, and sensitive.
Herve
V M
VM
Institut Pasteur, Bangui, Central African Republic.
Georges
A J
AJ
Massanga
M
M
Martin
P M
PM
D002511:Cephalosporins / Q000378:metabolism*; D005122:Exudates and Transudates / Q000382:microbiology; D006069:Gonorrhea / Q000382:microbiology*; D006801:Humans; D008297:Male; D009344:Neisseria gonorrhoeae / Q000201:enzymology / Q000302:isolation & purification*; D010405:Penicillinase / Q000032:analysis / Q000096:biosynthesis*; D011237:Predictive Value of Tests; D014521:Urethra / Q000382:microbiology*
D002511:Cephalosporins; C034313:pyridine-2-azo-4-dimethylaniline cephalosporin; D010405:Penicillinase