Synthesis of buffers for generating immobilized pH gradients. II: Basic acrylamido buffers.
Six basic acrylamido buffers, for isoelectric focusing in immobilized pH gradients, are described: a strong titrant (pK greater than 12), QAE-acrylamide, and five weaker bases, namely: 2-morpholinoethyl acrylamide (pK 6.2), 3-morpholinopropyl acrylamide (pK 7.0), N,N-dimethyl aminoethyl acrylamide (pK 8.5), N,N-dimethyl aminopropyl acrylamide (pK 9.3) and N,N-diethyl aminopropyl acrylamide (pK 10.3). Their synthesis and general properties are described.
Chiari
M
M
Department of Biomedical Science and Technology, University of Milano, Italy.
Casale
E
E
Santaniello
E
E
Righetti
P G
PG
D000178:Acrylamides / Q000138:chemical synthesis* / Q000737:chemistry; D002021:Buffers; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing
D000178:Acrylamides; D002021:Buffers; C059413:Immobiline