title: Quantitative analysis of chlortetracycline and related substances by high-performance liquid chromatography.
abstract: Isocratic high-performance liquid chromatography on Zorbax C8 7 microns allows quantitative determination of chlortetracycline, 4-epichlortetracycline, tetracycline, demethylchlortetracycline and isochlortetracycline using a mobile phase containing dimethylsulphoxide, 1 M perchloric acid and water (35:5:60). The minor impurities anhydrochlortetracycline and 4-epianhydrochlortetracycline, which are more strongly retained can be determined using a second isocratic system with a mobile phase containing more organic modifier. The method has been used for the comparison of official standards and for the analysis of a number of commercial samples.
authors.affiliation: Katholieke Universiteit Leuven, Instituut voor Farmaceutische Wetenschappen, Belgium.
authors.forename: N H
authors.initials: NH
authors.lastname: Khan
authors.forename: E
authors.initials: E
authors.lastname: Roets
authors.forename: J
authors.initials: J
authors.lastname: Hoogmartens
authors.forename: H
authors.initials: H
authors.lastname: Vanderhaeghe
mesh_terms: D002751:Chlortetracycline / Q000031:analogs & derivatives* / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D007202:Indicators and Reagents; D012015:Reference Standards; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet
chemical_list: D007202:Indicators and Reagents; D012997:Solvents; D002751:Chlortetracycline