Immunogold localisation of endogenous immunoglobulin-G in ultrathin frozen sections of the human placenta.
Endogenous immunoglobulin-G was localised in ultrathin frozen sections of human term placenta by use of an indirect immuno electron-histochemical methodology. Immunoreactivity of endogenous IgG to rabbit anti-human immunoglobulin-G antibody was visualised by use of protein-A--colloidal gold complex. Gold marked the syncytiotrophoblast in both coated and uncoated regions of the apical plasmalemma, in vesicles and multivesicular bodies, and in vesicles near the basal plasmalemma. Immunoreactivity was also seen in the interstitial space between the trophoblast and the fetal endothelial layer as well as in various types of vesicles within the endothelial cells. No immunoreactivity was seen in the intercellular clefts of the endothelium. The pattern of localisation observed is consistent with receptor-mediated uptake of immunoglobulin-G into the syncytiotrophoblast of the human placenta followed by release into the interstitial space and then vesicular transport through the endothelium.
Leach
L
L
Department of Obstetrics and Gynaecology, Charing Cross and Westminster Medical School, West London Hospital, United Kingdom.
Eaton
B M
BM
Firth
J A
JA
Contractor
S F
SF
D005260:Female; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000378:metabolism*; D007142:Immunoglobulin gamma-Chains / Q000378:metabolism*; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D010920:Placenta / Q000276:immunology* / Q000648:ultrastructure; D011247:Pregnancy
D007143:Immunoglobulin Heavy Chains; D007142:Immunoglobulin gamma-Chains