title: Biochemical analysis of bovine lens sections obtained by a new sectioning device.
abstract: One mm thick layers were reproducibly sliced from the punched-out inner cylinder of bovine lenses. The layers were obtained by a special designed freeze-sectioning device. Biochemical analysis of these layers revealed a detailed picture of the lenticular distribution of the amount of water-insoluble and urea-insoluble fractions. Furthermore, the distribution of the content of glucose, fructose and sorbitol was established. The results clearly show the usefulness of a microsectioning device to obtain layers for subsequent biochemical analysis which may lead to a comprehensive understanding of age- and/or cataract-related alterations within one lens.
authors.affiliation: Department of experimental Ophthalmology, University of Bonn, FRG.
authors.forename: G J
authors.initials: GJ
authors.lastname: Bessems
authors.forename: V
authors.initials: V
authors.lastname: Dragomirescu
authors.forename: B
authors.initials: B
authors.lastname: Möller
authors.forename: I
authors.initials: I
authors.lastname: Korte
authors.forename: O
authors.initials: O
authors.lastname: Hockwin
mesh_terms: D000375:Aging / Q000378:metabolism; D000818:Animals; D002417:Cattle; D003459:Crystallins / Q000378:metabolism; D004867:Equipment Design; D005629:Frozen Sections; D005632:Fructose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D007904:Lens Cortex, Crystalline / Q000378:metabolism*; D008867:Microtomy / Q000295:instrumentation*; D012995:Solubility; D013012:Sorbitol / Q000378:metabolism
chemical_list: D003459:Crystallins; D005632:Fructose; D013012:Sorbitol; D005947:Glucose