Characterization of two immunoglobulin VH genes that are homologous to human rheumatoid factors.
We isolated a VH1 germline gene (Humhv1263) that is closely related to the heavy chains of 2 human rheumatoid factors (RF), Bor and Kas. We also found that the 783 rearranged VH1 gene is actually 91-93% homologous to Bor and Kas, and it differs from a VH1 complementary DNA (51P1) by only a single base. Thus, 783 is likely to be the unmutated form of a germline VH gene that may encode the heavy chains of RF Bor and Kas and several other human monoclonal RF.
Chen
P P
PP
Department of Basic and Clinical Research, Research Institute of Scripps Clinic, La Jolla, California 92037.
Liu
M F
MF
Glass
C A
CA
Sinha
S
S
Kipps
T J
TJ
Carson
D A
DA
D000595:Amino Acid Sequence; D001482:Base Composition; D001483:Base Sequence; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D008969:Molecular Sequence Data; D012217:Rheumatoid Factor / Q000235:genetics*
D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D012217:Rheumatoid Factor