Identification of both Gi2 and a novel, immunologically distinct, form of Go in rat myometrial membranes.
Immunoblotting of rat myometrial membranes with an antiserum (SG1) which recognizes the alpha-subunits of both Gi1 and Gi2 indicated the presence of detectable levels of an apparently single form of some 40 kDa. A second antiserum (LE2) specific for Gi2 also recognized this protein, confirming its identity. Immunoblotting of the myometrial membranes with a series of antipeptide (OC1, IM1, ON1) and polyclonal (RV3) antisera, all of which recognize rat brain Go, produced a more complex pattern. Antisera OC1 and ON1 recognized a single polypeptide which essentially comigrated with rat brain Go. In contrast, antisera RV3 and IM1 did not recognize the myometrial protein. These data provide evidence for the presence of Gi2 and of a novel G-protein, related immunologically to Go, in rat myometrial membranes.
Milligan
G
G
Department of Biochemistry, University of Glasgow, Scotland.
Tanfin
Z
Z
Goureau
O
O
Unson
C
C
Harbon
S
S
D000818:Animals; D000918:Antibody Specificity; D002462:Cell Membrane / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D005260:Female; D019204:GTP-Binding Proteins / Q000032:analysis* / Q000276:immunology; D007106:Immune Sera; D015151:Immunoblotting / Q000379:methods; D046911:Macromolecular Substances; D009215:Myometrium / Q000378:metabolism*; D051381:Rats
D007106:Immune Sera; D046911:Macromolecular Substances; D019204:GTP-Binding Proteins