Altered formation of DNA replication intermediates in human 46 BR fibroblast cells hypersensitive to 3-aminobenzamide.
The appearance of DNA replication intermediates was investigated in a human fibroblast strain (46 BR) which is hypersensitive to the lethal effects of 3-aminobenzamide. 3-Aminobenzamide is an inhibitor of poly(ADP-ribose) synthetase and modulates DNA ligase activity. We detected the same intermediates (10 kb DNA and Okazaki-fragments) as in normal fibroblasts, but kinetics and amounts of intermediates were altered, either as a result of, or in order to overcome the defect in the cells.
Lönn
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U
Radiumhemmet, Karolinska Hospital, Stockholm, Sweden.
Lönn
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Nylen
U
U
Winblad
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G
D001549:Benzamides / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004261:DNA Replication / Q000187:drug effects*; D006801:Humans; D007700:Kinetics; D000067856:Poly(ADP-ribose) Polymerase Inhibitors
D001549:Benzamides; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; C025160:3-aminobenzamide