Reversible ADP-ribosylation of dinitrogenase reductase in a nifD- mutant of Rhodospirillum rubrum.
Dinitrogenase reductase from a Rhodospirillum rubrum strain lacking dinitrogenase was reversibly ADP-ribosylated in vivo in response to dark-light transitions. Addition of ammonia also led to ADP-ribosylation in this strain. These results demonstrate that reduced dinitrogenase is a satisfactory substrate for the reversible ADP-ribosylation system of R. rubrum in vivo.
Ludden
P W
PW
Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison 53706.
Lehman
L
L
Roberts
G P
GP
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D003624:Darkness; D017037:Dinitrogenase Reductase; D015180:Electrophoresis, Gel, Two-Dimensional; D004794:Enzyme Repression; D005288:Ferredoxins / Q000302:isolation & purification / Q000378:metabolism*; D005798:Genes, Bacterial; D008027:Light; D009154:Mutation; D009586:Nitrogen Fixation / Q000235:genetics*; D009591:Nitrogenase / Q000096:biosynthesis; D012247:Rhodospirillum rubrum / Q000235:genetics / Q000378:metabolism*
D017037:Dinitrogenase Reductase; D005288:Ferredoxins; D000246:Adenosine Diphosphate Ribose; D009591:Nitrogenase