Differences in 23 S rRNA-protein neighbourhood in Escherichia coli 70 S ribosomes and 70 S initiation complex. Probing by bifunctional Pt(II)-containing reagent.
rRNA-protein cross-links in free E. coli 35S-labeled 70 S ribosomes and in the initiation complex 35S-labeled 70 S ribosome.AUGU6.fMet-tRNA(fMet) were studied with the aid of a new type of binuclear Pt(II) compound - dichlorotetra-ammine(1,6-hexamethylenediaminediplatinum++ +) dichloride. The use of this reagent allowed us to reveal differences in the rRNA-protein neighbourhood in free 70 S ribosomes and in the initiation complex. Proteins L3, L6, L23 and L25 were shown to cross-link to 23 S rRNA only in the initiation complex, whereas proteins L1, L13, L14, L16, L17, L18, L22, L28 and S1 did so in both free ribosomes and the complex. 16 S rRNA was found to be cross-linked preferentially to a single protein, S1, in both states of the ribosomes.
Chistyakov
P G
PG
Novosibirsk Institute of Bioorganic Chemistry, Siberian Division USSR Academy of Sciences.
Venjaminova
A G
AG
Vladimirov
S N
SN
Graifer
D M
DM
Kazakov
S A
SA
Karpova
G G
GG
D002499:Centrifugation, Density Gradient; D003432:Cross-Linking Reagents; D015180:Electrophoresis, Gel, Two-Dimensional; D004926:Escherichia coli / Q000378:metabolism*; D009944:Organoplatinum Compounds / Q000494:pharmacology*; D012335:RNA, Ribosomal / Q000378:metabolism*; D012338:RNA, Ribosomal, 23S / Q000378:metabolism*; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism; D012358:RNA, Transfer, Met; D012360:RNA, Transfer, Phe / Q000378:metabolism; D012269:Ribosomal Proteins / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*; D013890:Thiourea / Q000494:pharmacology
D003432:Cross-Linking Reagents; D009944:Organoplatinum Compounds; D012335:RNA, Ribosomal; D012338:RNA, Ribosomal, 23S; D012346:RNA, Transfer, Amino Acyl; D012358:RNA, Transfer, Met; D012360:RNA, Transfer, Phe; D012269:Ribosomal Proteins; C024988:tRNA, formylmethionine-; C059267:dichlorotetraamine(1,6-hexamethylenediamine)diplatinum(II); D013890:Thiourea