High resolution 2-dimensional mapping of protease activities.
Proteases are secreted by wide variety of cells and are involved in many important processes. The qualitative analysis of these activities from small volumes of conditioned media is often difficult and limited to a description of apparent molecular weights. Here we report the application of a 2-dimensional system with the capability to resolve and identify subtle alterations of post translational modifications of individual activities.
Brown
D
D
Jane & Norman Neely Keratoconus Center, Department of Ophthalmology Research Cedars-Sinai Medical Center, Los Angeles CA.
Kenney
M C
MC
D015180:Electrophoresis, Gel, Two-Dimensional; D010450:Endopeptidases / Q000737:chemistry*; D006801:Humans; D008666:Metalloendopeptidases / Q000737:chemistry; D010449:Peptide Mapping; D010662:Phenylmercuric Acetate / Q000031:analogs & derivatives
C010024:4-aminophenylmercuriacetate; D010450:Endopeptidases; D008666:Metalloendopeptidases; D010662:Phenylmercuric Acetate