title: Nucleotide sequence of the aliphatic amidase regulator gene (amiR) of Pseudomonas aeruginosa.
abstract: The nucleotide sequence of a 1001 bp ClaI/XhoI DNA fragment encoding the amidase regulator gene (amiR) from Pseudomonas aeruginosa has been determined. The sequence derives from strain PAC433, a constitutive high expressing amidase mutant, and contains two overlapping open reading frames. Analysis of the sequence has identified one of the reading frames as amiR. The gene encodes a 196 amino acid polypeptide which shows a strong bias towards codons with G or C in the third position. The amiR gene shows no sequence homology with other bacterial regulator proteins.
authors.affiliation: Department of Biochemistry, University College London, England.
authors.forename: N
authors.initials: N
authors.lastname: Lowe
authors.forename: P M
authors.initials: PM
authors.lastname: Rice
authors.forename: R E
authors.initials: RE
authors.lastname: Drew
mesh_terms: D000581:Amidohydrolases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003062:Codon; D015252:Deoxyribonucleases, Type II Site-Specific; D005798:Genes, Bacterial; D005809:Genes, Regulator; D008969:Molecular Sequence Data; D008970:Molecular Weight; D014176:Protein Biosynthesis; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000235:genetics*
chemical_list: D003062:Codon; C032824:ATCGAT-specific type II deoxyribonucleases; C034510:CTCGAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; D000581:Amidohydrolases; C019665:amidase