Reaction on alternating GC oligonucleotide templates.
The condensation reactions of activated nucleotides, ImpN or 2-MeImpN, with the self-complementary ribo-octanucleotide GCGCGCGC or with the partially self-complementary heptanucleotide GCGCGCG were studied. The template-directed reaction of 2-MeImpC with the heptamer yields the 3'-5' octamer as the main product. All other reactions yield 2'-5'-linked octamers and pyrophosphates as major products. Surprisingly, 2-MeImpG facilitates the reaction of 2-MeImpC with the heptamer. Procedures for the analysis by gel electrophoresis of the oligomeric products obtained in reactions of this kind are described.
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Salk Institute for Biological Studies, San Diego, California 92138.
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D001345:Autoradiography; D001483:Base Sequence; D007202:Indicators and Reagents; D009843:Oligoribonucleotides / Q000138:chemical synthesis; D010761:Phosphorus Radioisotopes; D013329:Structure-Activity Relationship; D013698:Templates, Genetic
D007202:Indicators and Reagents; D009843:Oligoribonucleotides; D010761:Phosphorus Radioisotopes