Absence of synergism between tissue-type plasminogen activator and urokinase on plasminogen activation rate in plasma.
Effects of tissue-type plasminogen activator (t-PA), urokinase (u-PA) and their combinations on plasminogen activation rate (PAR) in plasma, in vitro were investigated. T-PA and u-PA over concentrations range of 10 U/ml to 50 U/ml induced a linear, concentration dependent increase in PAR. Combinations of t-PA and u-PA in ratios of 3/1, 1/1 and 1/3 induced additive but not synergistic effect in the activation of plasminogen. We conclude, therefore, that t-PA and u-PA do not act synergistically in the activation of plasminogen in plasma in vitro.
Rydzewski
A
A
Department of Physiology, Hamamatsu University, School of Medicine, Shizuoka-ken, Japan.
Takada
Y
Y
Takada
A
A
D002863:Chromogenic Compounds; D004357:Drug Synergism; D006801:Humans; D006868:Hydrolysis; D066298:In Vitro Techniques; D009842:Oligopeptides; D010958:Plasminogen / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology; D010959:Tissue Plasminogen Activator / Q000494:pharmacology*; D014568:Urokinase-Type Plasminogen Activator / Q000494:pharmacology*
D002863:Chromogenic Compounds; D009842:Oligopeptides; D011994:Recombinant Proteins; C018788:valyl-leucyl-lysine 4-nitroanilide; D010958:Plasminogen; D010959:Tissue Plasminogen Activator; D014568:Urokinase-Type Plasminogen Activator