title: Role of interferon-gamma in the modulation of the IgE response by 2,4-dinitrophenyl-Bordetella pertussis vaccine in the mouse.
abstract: Immunization of mice with 2,4-dinitrophenyl-Bordetella pertussis (DNP-BP) failed to induce anti-DNP IgE responses. Administration of DNP-BP induced, however, the formation of anti-DNP IgE B memory cells, as demonstrated by adoptive transfer. Furthermore, mice pretreated with DNP-BP and primed with 2 micrograms DNP-ovalbumin (OA) in alum 2 weeks later produced high day-7 anti-DNP IgE levels. These subsided to near undetectable levels by day 12-14. The transient expression of serum IgE levels was accompanied by normal levels of anti-DNP IgG. The anti-OA response induced as a result of priming with DNP-OA in alum was not affected by pretreatment with DNP-BP. IgG subclass analysis revealed that mice pretreated with DNP-BP had elevated levels of IgG2a and reduced levels of IgG1 as compared to control (TNP-keyhole limpet hemocyanin-pretreated) mice. Treatment of mice with an anti-interferon-gamma monoclonal antibody, shortly after immunization with DNP-BP, not only reduced anti-DNP IgG2a levels, but prevented the sharp anti-DNP IgE decline that occurred after priming with DNP-OA in alum. These results suggest that DNP-BP-induced interferon-gamma production modulates Ig isotype expression in vivo in an anti-gen-specific manner.
authors.affiliation: Department of Microbiology and Immunology, University of Western Ontario, London, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Hagen
authors.forename: N A
authors.initials: NA
authors.lastname: Essani
authors.forename: G H
authors.initials: GH
authors.lastname: Strejan
mesh_terms: D000818:Animals; D004136:Dinitrobenzenes / Q000276:immunology*; D007126:Immunoglobulin Allotypes / Q000032:analysis; D007073:Immunoglobulin E / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis; D007371:Interferon-gamma / Q000502:physiology*; D051379:Mice; D008808:Mice, Inbred CBA; D009578:Nitrobenzenes / Q000276:immunology*; D010567:Pertussis Vaccine / Q000276:immunology*
chemical_list: D004136:Dinitrobenzenes; D007126:Immunoglobulin Allotypes; D007074:Immunoglobulin G; D009578:Nitrobenzenes; D010567:Pertussis Vaccine; D007073:Immunoglobulin E; D007371:Interferon-gamma