title: Cytofluorometric study on lectin binding of isolated guinea pig keratinocytes.
abstract: Lectin binding was cytofluorometrically measured on fractionated keratinocytes of guinea pig. Free keratinocytes were obtained by treatment of EDTA and trypsin. After the treatment, they were separated into 3 fractions by centrifugation on a continuous colloidal silica (Percoll) density gradient. Cells in each fraction were stained by biotinyl lectins and avidin-FITC, and fluorescence intensity was measured by cytofluorometry. Results obtained indicate that little cell surface glycoconjugate is lost during the preparation of free keratinocytes.
authors.affiliation: Department of Dermatology, Kurume University School of Medicine, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Nomura
authors.forename: H
authors.initials: H
authors.lastname: Hachisuka
authors.forename: M
authors.initials: M
authors.lastname: Higuchi
authors.forename: Y
authors.initials: Y
authors.lastname: Sasai
mesh_terms: D000818:Animals; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006001:Glycoconjugates / Q000032:analysis*; D006168:Guinea Pigs; D015603:Keratinocytes / Q000166:cytology*; D037102:Lectins; D008297:Male
chemical_list: D006001:Glycoconjugates; D037102:Lectins