Immunophenotypic demonstration of two natural killer surface markers, H25 and H366, on fresh human leukemic cells.
Using a modified alkaline-phosphatase/antialkaline-phosphatase method for phenotyping fresh human leukemias, we could demonstrate peripheral blood and bone marrow-derived blast cells to specifically react with two monoclonal antibodies (MoAbs), H25 and H366, previously shown to recognize natural killer cells, activated T lymphocytes and a proportion of normal hematopoietic precursor cells. MoAbs H25 and H366 were found to identify the majority of leukemic cells in patients presenting with T-ALL, LGL leukemia, pre-B-ALL, CML, and AML, respectively.
Körfer
A
A
Abteilung Hämatologie und Onkologie, Medizinische Hochschule Hannover, FRG.
Kirchner
H
H
Schneekloth
C
C
Bührer
C
C
Wisniewski
D
D
Gulati
S
S
Clarkson
B
B
Knowles
R
R
Poliwoda
H
H
Atzpodien
J
J
D000818:Animals; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D006801:Humans; D007694:Killer Cells, Natural / Q000032:analysis*; D007938:Leukemia / Q000276:immunology*; D007943:Leukemia, Hairy Cell / Q000276:immunology; D007945:Leukemia, Lymphoid / Q000276:immunology; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000276:immunology; D015470:Leukemia, Myeloid, Acute / Q000276:immunology; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010641:Phenotype
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000954:Antigens, Surface