Isotype analysis of the anti-CENP-B anticentromere autoantibody: evidence for restricted clonality.
Utilizing the centromere B fusion protein (CENP-B) and specific, matched monoclonal antiisotype reagents in an enzyme-linked immunosorbent assay, we found that anti-CENP-B autoantibodies were skewed to the IgG1 isotype. The overall kappa:lambda light chain ratio was 2:1, although several individual sera showed a strong predominance of one of the light chains. Isoelectric focusing of light chain-skewed sera showed polyclonal patterns. Our findings are consistent with the anti-CENP-B autoantibody response being a chronic, antigen-driven response.
Eisenberg
R A
RA
Department of Medicine, University of North Carolina, Chapel Hill.
Earnshaw
W C
WC
Bordwell
B J
BJ
Craven
S Y
SY
Cheek
R
R
Rothfield
N F
NF
D001323:Autoantibodies / Q000145:classification / Q000276:immunology*; D002503:Centromere / Q000276:immunology*; D002875:Chromosomes / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000145:classification; D007132:Immunoglobulin Isotypes / Q000276:immunology*; D007145:Immunoglobulin kappa-Chains / Q000032:analysis; D007146:Immunoglobulin lambda-Chains / Q000032:analysis; D007526:Isoelectric Point; D012595:Scleroderma, Systemic / Q000276:immunology
D001323:Autoantibodies; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains