Effect of toxins on arachidonic acid metabolism in rat cultured pulmonary alveolar macrophages.
The action of a trichothecene (T-2), microcystin-LR and saxitoxin on arachidonic acid metabolism in cultured rat alveolar macrophages was studied. Pulmonary macrophages exposed to T-2 trichothecene were stimulated to synthesize and release large amount of thromboxane B2 (TxB2) and 6-Keto F1 alpha. Microcystin-LR induced significant release of prostaglandins F2 alpha (140%), PGE2 (175%) and TxB2 (169%) compared to controls. Saxitoxin induced TxB2 release by 37%. Arachidonic acid release was stimulated by all three toxins. The release of arachidonic acid and its metabolites in alveolar macrophages exposed to T-2 toxin was partially blocked by fluocinolone (1 microM). These results suggest that macrophages synthesize and release inflammatory mediators in response to toxin exposure, and fluocinolone may protect against T-2 toxicosis.
Naseem
S M
SM
U.S. Army Medical Research Institute of Infectious Diseases, Division of Pathophysiology, Fort Detrick, Frederick, Maryland 21701-5011.
Hines
H B
HB
Creasia
D A
DA
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D002478:Cells, Cultured; D005033:Ethylmaleimide / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D008387:Marine Toxins; D052998:Microcystins; D010456:Peptides, Cyclic; D011453:Prostaglandins / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D012530:Saxitoxin / Q000633:toxicity; D012588:Scintillation Counting; D013605:T-2 Toxin / Q000633:toxicity; D014118:Toxins, Biological / Q000633:toxicity*
D001095:Arachidonic Acids; D008387:Marine Toxins; D052998:Microcystins; D010456:Peptides, Cyclic; D011453:Prostaglandins; D014118:Toxins, Biological; D016718:Arachidonic Acid; D012530:Saxitoxin; D000001:Calcimycin; C057862:cyanoginosin LR; D013605:T-2 Toxin; D005033:Ethylmaleimide