title: Fate of attenuated schistosomula administered to mice by different routes, relative to the immunity induced against Schistosoma mansoni.
abstract: Newly transformed schistosomula and day 8 lung parasites, derived from optimally irradiated cercariae, were used to immunize groups of C57Bl/6 mice via 4 different injection routes. Schistosomula administered intradermally induced high levels of protection, comparable with that achieved by percutaneous vaccination. Intermediate levels were elicited by delivery of parasites via intraperitoneal or intratracheal routes. In contrast, intravenous injection of schistosomula to the lungs resulted in little or no resistance. Attenuated day 8 schistosomula administered intradermally were at least as immunogenic as irradiated cercariae. The fate of radio-isotope labelled attenuated lung schistosomula, injected via the various routes, was examined by compressed organ autoradiography. After intradermal vaccination, a proportion of parasites migrated from the site of injection to the draining lymph node and lungs. Conversely, schistosomula administered via the other 3 routes persisted to varying degrees at the injection site, but little onward migration was observed. We suggest that successful vaccination requires that some attenuated parasites migrate to, and sequester in, lymph nodes draining the vaccination site; persistence at the site of administration alone is not an adequate stimulus.
authors.affiliation: Department of Biology, University of York.
authors.forename: P S
authors.initials: PS
authors.lastname: Coulson
authors.forename: A P
authors.initials: AP
authors.lastname: Mountford
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D005260:Female; D007110:Immunity, Active; D007271:Injections, Intradermal; D007274:Injections, Intraperitoneal; D007275:Injections, Intravenous; D008168:Lung / Q000469:parasitology; D008198:Lymph Nodes / Q000469:parasitology; D051379:Mice; D008810:Mice, Inbred C57BL; D012550:Schistosoma mansoni / Q000276:immunology* / Q000302:isolation & purification; D012555:Schistosomiasis mansoni / Q000276:immunology* / Q000517:prevention & control; D013997:Time Factors; D014611:Vaccination; D014613:Vaccines, Attenuated / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D014613:Vaccines, Attenuated