title: Interaction between endometrium epithelial cells (SNG-M) and teratocarcinoma cells (HT-H) differentiated into trophoblast.
abstract: Many of the human teratocarcinoma cell lines resemble cells of preblastocyst to trophectoderm. The use of these cell lines is, however, limited since they differentiate poorly in vitro. HT-H cell line might be useful as it differentiates into syncytiotrophoblast. Because syncytiotrophoblast of the blastocyst are the cells which attach to the maternal endometrium epithelium during implantation, HT-H cells were examined for their adhesion to SNG-M cells, which feature exhibit cells of endometrium epithelium. Differentiated HT-H cells attached to SNG-M cells, and between these two cells desmosomes and adherent junctions were developed. Undifferentiated HT-H cells did not directly attach to SNG-M cells. Cell-to-cell adhesions are often mediated by the homophilic adhesion molecules present in both cells. As the first step in the investigation of adhesion between SNG-M and HT-H cells, we examined adhesion between SNG-M cells themselves. Calcium had no effect on aggregation of SNG-M cells, which may exclude the involvement of cadherin type molecule in this system. Development of monoclonal antibodies that bind to the cell surface and inhibit aggregation of SNG-M cells, and SNG-M to HT-H cells will allow us to identify the adhesion molecule(s) involved in ovum implantation in utero.
authors.affiliation: La Jolla Cancer Research Foundation, California 92037.
authors.forename: M N
authors.initials: MN
authors.lastname: Fukuda
mesh_terms: D000606:Amino Sugars / Q000378:metabolism / Q000502:physiology; D002448:Cell Adhesion; D002449:Cell Aggregation; D002450:Cell Communication; D002454:Cell Differentiation; D010064:Embryo Implantation; D004717:Endometrium / Q000166:cytology* / Q000378:metabolism; D005260:Female; D006801:Humans; D011134:Polysaccharides / Q000378:metabolism / Q000502:physiology; D013724:Teratoma / Q000378:metabolism / Q000473:pathology*; D014327:Trophoblasts; D014407:Tumor Cells, Cultured
chemical_list: D000606:Amino Sugars; D011134:Polysaccharides; C039448:lactosaminoglycan