Isolation and characterization of a fruit-specific cDNA and the corresponding genomic clone from tomato.
Differential screening of a cDNA bank constructed from ripe tomato fruit mRNA allowed the isolation of cDNA clone 2A11 which is entirely fruit-specific, is expressed at steadily increasing levels from anthesis to breaker, and accounts for approximately 1% of the messenger RNA in mature tomato fruit. A genomic clone corresponding to the 2A11 cDNA was isolated from a tomato genomic library. Sequence comparison of the cDNA clone with the genomic clone shows they are identical over the shared region with the genomic clone possessing a single large intron near the 5' end of the message. The open reading frame of 2A11 would encode a sulfur-rich polypeptide 96 amino acids in length. The identity of the putative protein is unknown. In situ hybridization shows that the 2A11 message is found throughout the pericarp cells in a tomato fruit. In contrast, in situ hybridization of early ripening stages with a polygalacturonase probe shows higher mRNA levels in cells of the outer pericarp and cells surrounding the vascular regions of the pericarp.
Pear
J R
JR
Calgene, Inc., Davis, CA 95616.
Ridge
N
N
Rasmussen
R
R
Rose
R E
RE
Houck
C M
CM
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D005638:Fruit / Q000235:genetics / Q000254:growth & development; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D063245:Plant Development; D010940:Plant Proteins / Q000235:genetics; D010944:Plants / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping
D010940:Plant Proteins; D012333:RNA, Messenger; D004247:DNA