title: Catalase and glutathione peroxidase are equally active in detoxification of hydrogen peroxide in human erythrocytes.
abstract: Genetic deficiencies of glucose-6-phosphate dehydrogenase (G6PD) and NADPH predispose affected erythrocytes to destruction from peroxides. Conversely, genetic deficiencies of catalase do not predispose affected erythrocytes to peroxide-induced destruction. These observations have served to strengthen the assumption that the NADPH/glutathione/glutathione peroxidase pathway is the principal means for disposal of H2O2 in human erythrocytes. Recently, however, mammalian catalase was found to have tightly bound NADPH and to require NADPH for the prevention and reversal of inactivation by its toxic substrate (H2O2). Since both catalase and the glutathione pathway are dependent on NADPH for function, this finding raises the possibility that both mechanisms destroy H2O2 in human erythrocytes. A comparison of normal and acatalasemic erythrocytes in the present study indicated that catalase accounts for more than half of the destruction of H2O2 when H2O2 is generated at a rate comparable to that which leads to hemolysis in G6PD- deficient erythrocytes.
authors.affiliation: Division of Hematology, University of Genoa, Italy.
authors.forename: G F
authors.initials: GF
authors.lastname: Gaetani
authors.forename: S
authors.initials: S
authors.lastname: Galiano
authors.forename: L
authors.initials: L
authors.lastname: Canepa
authors.forename: A M
authors.initials: AM
authors.lastname: Ferraris
authors.forename: H N
authors.initials: HN
authors.lastname: Kirkman
mesh_terms: D002245:Carbon Dioxide / Q000097:blood; D002250:Carbon Radioisotopes; D002374:Catalase / Q000097:blood*; D004912:Erythrocytes / Q000201:enzymology / Q000378:metabolism*; D005955:Glucosephosphate Dehydrogenase Deficiency / Q000097:blood / Q000201:enzymology; D005979:Glutathione Peroxidase / Q000097:blood*; D006801:Humans; D006861:Hydrogen Peroxide / Q000096:biosynthesis / Q000097:blood* / Q000502:physiology; D008658:Inactivation, Metabolic; D007424:Intracellular Fluid / Q000201:enzymology; D008297:Male; D009243:NAD / Q000502:physiology; D010427:Pentose Phosphate Pathway
chemical_list: D002250:Carbon Radioisotopes; D009243:NAD; D002245:Carbon Dioxide; D006861:Hydrogen Peroxide; D002374:Catalase; D005979:Glutathione Peroxidase