title: Silicone-grease-based immobilisation method for the preparation of enzyme electrodes.
abstract: An approach to the construction of amperometric biosensors based on the incorporation of an enzyme in silicone grease and using the grease to fill micropores on a graphite surface is described. The enzyme-grease electrode concept, illustrated with the enzyme tyrosinase, offers a very simple, rapid and inexpensive approach to the fabrication of enzyme electrodes. The tyrosinase electrode responds very rapidly to dynamic changes in the concentration of phenolic compounds. A response time (t95%) as low as 5 s has been determined. With flow injection, 120 samples per hour can be processed with a relative standard deviation of 2.4%. The electrode remains active for about 12 d. The detection limit for dopamine is 6 x 10(-6) M. This method of biosensor construction should be applicable to other enzyme-substrate systems.
authors.forename: M P
authors.initials: MP
authors.lastname: Connor
authors.forename: J
authors.initials: J
authors.lastname: Sanchez
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: M R
authors.initials: MR
authors.lastname: Smyth
authors.forename: S
authors.initials: S
authors.lastname: Mannino
mesh_terms: D004156:Catechol Oxidase; D004566:Electrodes; D004800:Enzymes, Immobilized; D014442:Monophenol Monooxygenase; D009638:Norepinephrine / Q000032:analysis; D012828:Silicones
chemical_list: D004800:Enzymes, Immobilized; D012828:Silicones; D004156:Catechol Oxidase; D014442:Monophenol Monooxygenase; D009638:Norepinephrine