Detection of serum HIV-Ag related to the major core protein (p24) in persons at risk for AIDS.
A commercial enzyme immunoassay was utilized for the detection of the human immunodeficiency virus antigen (p24) in serum samples from persons at risk for AIDS. The test demonstrated sensitivity to 20 pg/ml of serum antigen. The results obtained showed 14.63% of the subjects (Ss) with persistent generalized lymphadenopathy or asymptomatic Ss, and 69.23% of the Ss with AIDS related complex (ARC) or AIDS to be positive for HIV-Ag. In addition, there were no false-positives in the control serum samples collected from seronegative individual. The close correlation observed between HIV-Ag presence and advanced stages of the disease, stresses the diagnostic importance of this viral marker and its usefulness in the follow-up of Ss with HIV infection.
Borghi
V
V
Department of Infectious Diseases, University of Modena, Italy.
De Rienzo
B
B
Pietrosemoli
P
P
Pecorari
M
M
Mongiardo
N
N
Pellegrino
F
F
Zanchetta
G P
GP
Lami
G
G
Squadrini
F
F
D000386:AIDS-Related Complex / Q000175:diagnosis / Q000276:immunology; D000163:Acquired Immunodeficiency Syndrome / Q000175:diagnosis* / Q000276:immunology; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D015483:HIV Antibodies / Q000032:analysis; D015488:HIV Antigens / Q000032:analysis* / Q000276:immunology; D016655:HIV Core Protein p24; D006801:Humans; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D012191:Retroviridae Proteins / Q000032:analysis* / Q000276:immunology; D012307:Risk Factors
D015483:HIV Antibodies; D015488:HIV Antigens; D016655:HIV Core Protein p24; D011933:Reagent Kits, Diagnostic; D012191:Retroviridae Proteins