Swelling of the Golgi apparatus and decrease of galactosyltransferase in polyamine-deficient bovine lymphocytes and epithelium of mouse small intestine.
When bovine small lymphocytes stimulated by concanavalin A were treated with inhibitors (alpha-difluoromethylornithine (5 mM) and ethylglyoxal bis(guanylhydrazone) (100 microM)] of polyamine biosynthesis, swelling of the Golgi apparatus was observed. This was accompanied by decreases in the amount of the Golgi apparatus and of the specific activity of galactosyltransferase. Both spermidine and spermine, at physiological concentrations, stimulated galactosyltransferase activity 2-3-fold. When mice were treated with these inhibitors, the following changes were observed in the epithelial cells of small intestine: swelling of the Golgi apparatus; decrease in the amount of the Golgi apparatus; and decrease of galactosyltransferase activity.
Sakamaki
Y
Y
Faculty of Pharmaceutical Sciences, Inohana Campus, Chiba, Japan.
Terao
K
K
Ito
E
E
Kashiwagi
K
K
Igarashi
K
K
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D000518:Eflornithine / Q000494:pharmacology; D004848:Epithelium / Q000201:enzymology; D005700:Galactosyltransferases / Q000037:antagonists & inhibitors*; D006056:Golgi Apparatus / Q000187:drug effects* / Q000648:ultrastructure; D007413:Intestinal Mucosa / Q000201:enzymology; D007421:Intestine, Small / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D008935:Mitoguazone / Q000031:analogs & derivatives / Q000494:pharmacology; D011073:Polyamines / Q000096:biosynthesis*; D013095:Spermidine / Q000494:pharmacology; D013096:Spermine / Q000494:pharmacology
D011073:Polyamines; D003208:Concanavalin A; C041127:ethylglyoxal bis(guanylhydrazone); D013096:Spermine; D005700:Galactosyltransferases; D008935:Mitoguazone; D013095:Spermidine; D000518:Eflornithine