The class II genes of the rat MHC.
Genes that encode class II Ag from the MHC of the rat, the RT1 region, have been isolated as a series of cosmid clones. The cosmids define two clusters, each of which contains three identifiable sequences; one homologous to alpha-chain and two to beta-chain genes. Both the serologically identified rat class II Ag have been expressed in mouse L cell fibroblasts after the introduction of each alpha-chain gene along with a beta-chain gene from the same cluster. There are substantial homologies to the I region of the mouse H-2 complex in the presence, location, orientation, and expression of the six identified sequences from the rat RT1, supporting the view that the overall organization of the two gene complexes has remained conserved since the species separated.
Diamond
A G
AG
Department of Immunology, A.F.R.C. Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, England.
Hood
L E
LE
Howard
J C
JC
Windle
M
M
Winoto
A
A
D000818:Animals; D003001:Cloning, Molecular; D003360:Cosmids; D005802:Genes, MHC Class II; D006183:H-2 Antigens / Q000235:genetics; D006649:Histocompatibility Antigens / Q000235:genetics*; D051379:Mice; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D051381:Rats; D011919:Rats, Inbred Strains / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D006183:H-2 Antigens; D006649:Histocompatibility Antigens; C038353:histocompatibility antigens RT, rat