title: Restriction map of mycobacteriophage D29 and its deletion mutant F5.
abstract: A physical map of mycobacteriophage D29 was constructed, including positions for 25 restriction sites for 9 endunocleasic enzymes. D29 DNA contains about 48 150 bp. Analysis of a deletion mutant (F5) has allowed to determine the location of two non essential regions in the genome, allowing further insertion of foreign genes and construction of cosmids.
authors.affiliation: Unité de Tuberculose et Mycobactéries, Paris, France.
authors.forename: R
authors.initials: R
authors.lastname: Lazraq
authors.forename: J
authors.initials: J
authors.lastname: Moniz-Pereira
authors.forename: S
authors.initials: S
authors.lastname: Clavel-Sérès
authors.forename: F
authors.initials: F
authors.lastname: Clément
authors.forename: H L
authors.initials: HL
authors.lastname: David
mesh_terms: D002872:Chromosome Deletion; D003360:Cosmids; D004279:DNA, Viral / Q000235:genetics; D005822:Genetic Vectors; D009154:Mutation; D009160:Mycobacteriophages / Q000235:genetics*; D009161:Mycobacterium / Q000235:genetics*; D015183:Restriction Mapping; D014764:Viral Proteins / Q000235:genetics / Q000302:isolation & purification; D015678:Viral Structural Proteins
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins; D015678:Viral Structural Proteins