Synthesis and pathway of Luciola mingrelica firefly luciferase in Xenopus laevis frog oocytes and in cell-free systems.
Poly (A+)RNA was isolated from the lanterns of adult fireflies of L. mingrelica. The poly (A+)RNA was translated in a cell-free translation mixture from rabbit reticulocyte and from Krebs II mouse ascites cells and in Xenopus laevis frog oocytes. The synthesis of the enzymatically active firefly luciferase was demonstrated in all systems. In the cell-free systems a maximal quantity of luciferase is synthesized during the first 1-2 h, then the decreasing activity of luciferase is observed. The optimal concentration of mRNA for translation in the mouse ascites cell extract was found. The kinetics of the synthesis of the luciferase, its pathway and stability in X. laevis oocytes was studied. It was observed that luciferase is secreted from oocytes into the incubation medium.
Kutuzova
G D
GD
Department of Chemistry, Lomonosov Moscow State University, USSR.
Skripkin
E A
EA
Tarasova
N I
NI
Ugarova
N N
NN
Bogdanov
A A
AA
D000818:Animals; D002474:Cell-Free System; D004175:Diptera / Q000201:enzymology*; D005260:Female; D066298:In Vitro Techniques; D007700:Kinetics; D008156:Luciferases / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D009865:Oocytes / Q000201:enzymology; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000235:genetics; D014982:Xenopus laevis
D012333:RNA, Messenger; D008156:Luciferases