Morphologic changes of K-Sol preserved human corneas.
Supplementation of tissue culture medium with chondroitin sulfate has been shown to enhance donor corneal preservation. We assessed the efficacy of one of these chondroitin-supplemented media (K-Sol) in comparison with McCarey-Kaufman (MK) medium in maintaining corneal cellular morphology. Thirty-six human corneas, obtained within 8.6 h after death, were placed into K-Sol medium for up to 20 days preservation, and five paired control corneas were placed into MK medium for up to 6 days preservation. Specular photomicrographs were obtained every second to third day for a predetermined storage interval, then studied morphologically in a masked protocol by light microscopy, transmission electron microscopy, and scanning electron microscopy. Endothelial cell loss by specular microscopy averaged 5.8% after 1 week (6 to 8 days) and 7.4% after 13 days in K-Sol medium. Epithelial changes were erratic throughout the 20 day K-Sol preservation period. However, substantial keratocyte changes occurred after 10 days, and endothelial morphology uniformly deteriorated after 17 days. The morphologic data suggest that human corneas may be able to be preserved in K-Sol medium at 4 degrees C for up to 10 days but should be cautiously used thereafter.
Farge
E J
EJ
Cullen Eye Institute, Baylor College of Medicine, Houston, Texas 77030.
Fort
R A
RA
Wilhelmus
K R
KR
Gilbert
M L
ML
Mehta
R S
RS
Kretzer
F L
FL
D000328:Adult; D000368:Aged; D002452:Cell Count; D002807:Chondroitin / Q000031:analogs & derivatives*; D002809:Chondroitin Sulfates; D003315:Cornea / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D003319:Corneal Stroma / Q000166:cytology; D004728:Endothelium, Corneal / Q000166:cytology; D004847:Epithelial Cells; D005260:Female; D005404:Fixatives; D006531:HEPES; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D010879:Piperazines; D013997:Time Factors; D014021:Tissue Preservation
D005404:Fixatives; C051174:K-Sol preserving medium; D010879:Piperazines; D002807:Chondroitin; D002809:Chondroitin Sulfates; D006531:HEPES