title: Isolation of creatine kinase BB isoenzyme with high specific activity and adequate purity for radioimmunoassay from human placenta on preparative polyacrylamide gel electrophoresis.
abstract: This report describes the procedures for isolation of creatine kinase BB isoenzyme (CK-BB) from human placenta on preparative polyacrylamide gel electrophoresis. 2.5 mg of CK-BB was purified from a 100-g portion of the human placenta, which had a mean specific activity of 957 kU/g and a mean yield of 16%. The placenta CK-BB exhibited single protein bands on several electrophoretic techniques. In addition, both of the placenta and brain CK-BB preparations were individually iodinated and the identical immunological properties of both the CK-BB preparations were confirmed in radioimmunoassay.
authors.affiliation: Shanghai Institute of Cardiovascular Diseases, People's Republic of China.
authors.forename: J G
authors.initials: JG
authors.lastname: Geng
authors.forename: H Z
authors.initials: HZ
authors.lastname: Cheng
authors.forename: Y F
authors.initials: YF
authors.lastname: Yang
authors.forename: Z H
authors.initials: ZH
authors.lastname: Qian
authors.forename: C Y
authors.initials: CY
authors.lastname: Jiang
mesh_terms: D001345:Autoradiography; D003402:Creatine Kinase / Q000032:analysis / Q000302:isolation & purification*; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D005779:Immunodiffusion; D007527:Isoenzymes; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D011863:Radioimmunoassay
chemical_list: D007527:Isoenzymes; D003402:Creatine Kinase