Recent developments in the molecular genetics and biology of self-incompatibility.
A summary of recent work on molecular aspects of self-incompatibility in Nicotiana alata is presented. The amino acid sequences of style proteins corresponding to different S-alleles of N. alata have a high level of homology in some regions and are variable in other regions. The regions of homology include N-terminal sequences as well as most of the glycosylation sites and cysteine residues. The glycosyl substituents may consist of a number of 'glycoforms'. The isolated style S-glycoproteins inhibit in vitro growth of pollen tubes. The S-glycoproteins tested inhibited the growth of pollen of several S-genotypes, and there was some specificity in the interaction. Heat treatment of the isolated S-glycoproteins dramatically increased their activity as inhibitors of pollen tube growth, although the specificity in the interaction was lost. The nature of the S-allele products in pollen is not yet established.
Clarke
A E
AE
Plant Cell Biology Research Centre, School of Botany, University of Melbourne, Parkville, Victoria, Australia.
Anderson
M A
MA
Atkinson
A
A
Bacic
A
A
Ebert
P R
PR
Jahnen
W
W
Lush
W M
WM
Mau
S L
SL
Woodward
J R
JR
D000483:Alleles; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D006023:Glycoproteins / Q000235:genetics; D008967:Molecular Biology; D010940:Plant Proteins / Q000235:genetics; D010944:Plants / Q000235:genetics*; D010947:Plants, Toxic; D012098:Reproduction / Q000235:genetics; D014026:Nicotiana / Q000235:genetics
D006023:Glycoproteins; D010940:Plant Proteins; D004247:DNA