Effect of glutaraldehyde fixation on cell surface binding capacity of Candida albicans.
The ability of viable and glutaraldehyde-fixed, stationary-phase yeast cells of Candida albicans to bind concanavalin A and monospecific antiserum for antigenic factor 1 was examined. Both fluorescence flow cytometric analysis and transmission electron microscopy indicated that glutaraldehyde-fixed cells bound less of the two reagents than did unfixed viable cells.
Mleczko
J
J
Department of Microbiology, Texas Tech University Health Sciences Center, Lubbock 79430.
Litke
L L
LL
Larsen
H S
HS
Chaffin
W L
WL
D000447:Aldehydes; D000946:Antigens, Fungal / Q000276:immunology / Q000378:metabolism*; D002176:Candida albicans / Q000378:metabolism* / Q000648:ultrastructure; D002473:Cell Wall / Q000378:metabolism / Q000648:ultrastructure; D005404:Fixatives; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D005976:Glutaral; D008351:Mannans / Q000276:immunology / Q000378:metabolism; D011952:Receptors, Concanavalin A / Q000187:drug effects* / Q000648:ultrastructure
D000447:Aldehydes; D000946:Antigens, Fungal; D005404:Fixatives; D008351:Mannans; D011952:Receptors, Concanavalin A; D005976:Glutaral