Increased arachidonic acid uptake by platelets from insulin-dependent diabetics and diabetic rats.
14C-Arachidonic acid uptake was measured in platelets obtained from 15 insulin-dependent diabetic and 17 control subjects and in 12 streptozotocin-diabetic and 21 control rats. The 14C-arachidonic acid uptake, expressed as pmol/10(8) platelets/min, mean +/- SEM, was significantly higher in platelets from diabetic subjects (2.80 +/- 0.23) and diabetic rats (1.73 +/- 0.20) than in the control subjects (2.29 +/- 0.15) and the control rats (1.35 +/- 0.08). No significant correlations were found between arachidonic acid uptake and glucose, total cholesterol or triglyceride plasma levels in either rats or humans. Arachidonic acid uptake was inhibited by indometacin but not by nordihydroguaiaretic acid, in diabetic as well as in control subjects. The present results suggest that the increased arachidonic acid uptake by platelets from insulin-dependent diabetic patients and streptozotocin-diabetic rats depends on their increased platelet arachidonic acid utilization through the cyclooxygenase pathway.
Perez
C
C
Department of Physiology, Faculty of Medicine, University of Extremadura, Badajoz, Spain.
Ramiro
J M
JM
Campillo
J E
JE
D000293:Adolescent; D000328:Adult; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000097:blood*; D001692:Biological Transport; D001786:Blood Glucose / Q000032:analysis; D001792:Blood Platelets / Q000378:metabolism*; D002250:Carbon Radioisotopes; D002648:Child; D002784:Cholesterol / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000097:blood*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D014280:Triglycerides / Q000097:blood
D001095:Arachidonic Acids; D001786:Blood Glucose; D002250:Carbon Radioisotopes; D014280:Triglycerides; D016718:Arachidonic Acid; D002784:Cholesterol