Extent of formation of a dimeric adenine photoproduct in polynucleotides and DNA.
Quantum yields are reported for the formation of a dimeric adenine photoproduct, A = A, in adenine homopolymers and DNA irradiated at 254 nm. The A = A content of irradiated samples was assayed by using reversed-phase HPLC to isolate the 4,6-diamino-5-guanidinopyrimidine (DGPY) which is produced from A = A on acid hydrolysis. Acid hydrolysates derived from DNA radiolabelled with [14C] 2'-deoxyadenosine were spiked with unlabelled DGPY before fractionation on HPLC and the recovered material was further purified by chromatography on Sephadex G-10 followed by co-crystallization with DGPY sulphate. Although A = A is formed with a relatively high quantum yield of 1.6 X 10(-3) mol einstein-1 in single-stranded poly(dA) the photoaddition reaction is strongly quenched in base-paired poly(dA).poly(dT) and undetectable in poly(rA).poly(dT). Respective quantum yields of 6 X 10(-5) and 9 X 10(-6) were estimated for the formation of A = A in single- and double-stranded E. coli DNA implying that the photoproduct has very limited biological significance. From studies with d(ApG), d(GpA), ApG, GpA, d(A)20 and d(A4G)4 it is concluded that adjacent guanine and adenine bases do not form a photoadduct analogous to A = A and also that guanine residues have no local or long-range quenching effect on photodimerization within A-A doublets.
Sharma
N D
ND
Biochemistry Department, Queen's University, Belfast, U.K.
Davies
R J
RJ
D000225:Adenine / Q000096:biosynthesis*; D002851:Chromatography, High Pressure Liquid; D004269:DNA, Bacterial / Q000528:radiation effects*; D010777:Photochemistry; D011061:Poly A / Q000528:radiation effects*; D011119:Polynucleotides / Q000032:analysis / Q000528:radiation effects; D014466:Ultraviolet Rays
D004269:DNA, Bacterial; D011119:Polynucleotides; D011061:Poly A; D000225:Adenine