Variant human neuroblastoma cell lines resistant to the differentiation-inducing effect of interferon-gamma.
Human recombinant interferon, (rIFN)-gamma, induced a human neuroblastoma cell line GOTO to differentiate, but neither rIFN-alpha A nor -beta did. To elucidate the mechanism of this rIFN-gamma-specific differentiation-inducing effect, we established two rIFN-gamma-resistant variant GOTO clones. They were insensitive to the growth-inhibitory and differentiation-inducing effect of 1 X 10(3) IU/ml rIFN-gamma. They were slightly sensitive to the growth-inhibitory effect of rIFN-gamma (2 X 10(4) IU/ml). Parental GOTO cells were very insensitive to the antivesicular stomatitis virus (VSV) effect of all three types of rIFNs and even 2 X 10(4) IU/ml rIFN-gamma could not inhibit the cytopathic effect of 5 TCID50 VSV by 50%. The degree of this insensitivity was the same in the variant GOTO cells as in the parental GOTO cells.
Higuchi
T
T
Department of Microbiology, Kyoto Prefectural University of Medicine, Japan.
Tanaka
A
A
Hiratani
H
H
Watanabe
H
H
Imanishi
J
J
D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects* / Q000473:pathology; D004351:Drug Resistance / Q000235:genetics; D014644:Genetic Variation; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D009447:Neuroblastoma / Q000473:pathology*; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured
D011994:Recombinant Proteins; D007371:Interferon-gamma