Differences in response of acetylcholinesterase to diisopropylfluorophosphate in the mesencephalic raphe region. A microelectrophoretic, histochemical, and biochemical study in albino rats.
To demonstrate the DFP-effects on the AChE of the mesencephalic raphe region albino rats were investigated using the pharmaco-histochemical method of Butcher et al. (1975). At several times after 1 mg DFP/kg body weight, the histochemical reactions in certain AChE-containing neurons were measured semiquantitatively and compared with biochemical data. Additionally, the multiple forms of water-soluble AChE were separated by microelectrophoresis. Only the electrophoretical results indicate changes of the structural AChE composition 5 d after DFP administration.
Seidel
J
J
Institute of Pharmacology and Toxicology, Karl Marx University Leipzig, GDR.
Scheibler
P
P
Müller
M
M
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D005260:Female; D006651:Histocytochemistry; D007531:Isoflurophate / Q000494:pharmacology*; D007700:Kinetics; D009474:Neurons / Q000187:drug effects / Q000201:enzymology*; D011903:Raphe Nuclei / Q000166:cytology / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values
D007531:Isoflurophate; D000110:Acetylcholinesterase