Allo and auto-antibodies against platelet antigens: a new immunoenzymatic assay for their detection and quantitation.
A new immunoenzymatic assay for the detection of serum-free and platelet-associated (PA) antibodies is described. This method evidences the platelet bound IgG employing the biotin-avidin system. Following a common sensitization step, the PA-IgG can be directly detected on the cell membranes, and their amount can be evaluated by an anti-globulin consumption assay. In comparison with the platelet suspension immunofluorescence test (PSIFT), the ELISA assay is a little less specific but it is more sensitive: less than 1 ng/ml of IgG can be detected. The number of IgG molecules/platelet detected investigating normal and pathological sera is higher than that reported by others: such results are probably related to the nonhomogeneity of immunoglobulins implicated in the assay.
Borzini
P
P
Radaelli
C
C
Assali
G
G
Ripamonti
M
M
Cazzaniga
G
G
D001323:Autoantibodies / Q000032:analysis*; D001792:Blood Platelets / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007518:Isoantibodies / Q000032:analysis*
D001323:Autoantibodies; D007518:Isoantibodies