Chromatography of human plasma on aminohexyl Sepharose: separation of factor VIII/vWf and behaviour of factors II, VII, IX and X and antithrombin III.
Human plasma was chromatographed under different experimental conditions on aminohexyl Sepharose. A strong retention of factor VIII/vWf and of factors VII, II, IX and X was observed. A satisfactory stabilization of eluted factor VIII clotting activity was obtained after addition of albumin and sucrose to the buffers used during chromatography. Albumin and antithrombin III were found apparently intact in the column effluent.
Ruttyn
Y
Y
Université de Technologie de Compiègne, Centre de Recherches de Royallieu, France.
Brandin
M P
MP
Vijayalakshmi
M A
MA
D000418:Albumins; D000990:Antithrombin III / Q000032:analysis*; D001779:Blood Coagulation Factors / Q000032:analysis*; D002021:Buffers; D002845:Chromatography / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D005164:Factor IX / Q000032:analysis; D005167:Factor VII / Q000032:analysis; D005169:Factor VIII / Q000032:analysis; D005170:Factor X / Q000032:analysis; D006801:Humans; D011516:Prothrombin / Q000032:analysis; D012685:Sepharose / Q000031:analogs & derivatives*; D013395:Sucrose; D014841:von Willebrand Factor / Q000302:isolation & purification*
D000418:Albumins; D001779:Blood Coagulation Factors; D002021:Buffers; D014841:von Willebrand Factor; D013395:Sucrose; C024903:aminohexyl-sepharose; D000990:Antithrombin III; D005167:Factor VII; D011516:Prothrombin; D005169:Factor VIII; D005164:Factor IX; D005170:Factor X; D012685:Sepharose