In vitro and in vivo toxicity of T-2 toxin, a Fusarium mycotoxin, to mouse peritoneal macrophages.
The effects of T-2 toxin on mouse peritoneal macrophages were investigated. Scanning electron microscopy of macrophages treated in vitro with T-2 toxin revealed retraction of pseudopodia. Protein synthesis was inhibited after in vitro contact with T-2 toxin but was not affected 24 h after injection of a sublethal dose of toxin into mice. There was reduction in the phagocytosis of Pseudomonas aeruginosa when macrophages were exposed in vitro to T-2 toxin and when mice were injected with T-2 toxin. Clearance of colloidal carbon was not modified after T-2 toxin injection, whereas spleen weight was decreased 24 h after T-2 injection. T-2 toxin enhanced the mortality of mice infected with Salmonella typhimurium C5S when it was administered 24 h prior to oral challenge with the bacterium.
Vidal
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Unité de Microbiologie, Centre de Recherches du Service de Santé des Armées, La Tronche, France.
Mavet
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D000818:Animals; D002244:Carbon / Q000378:metabolism; D002478:Cells, Cultured; D005670:Fusarium / Q000276:immunology* / Q000502:physiology; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism / Q000648:ultrastructure; D008657:Metabolic Clearance Rate; D051379:Mice; D008807:Mice, Inbred BALB C; D010529:Peritoneal Cavity; D010587:Phagocytosis; D011500:Protein Synthesis Inhibitors / Q000633:toxicity; D011550:Pseudomonas aeruginosa / Q000276:immunology; D012486:Salmonella typhimurium / Q000276:immunology; D012717:Sesquiterpenes / Q000633:toxicity*; D013605:T-2 Toxin / Q000008:administration & dosage / Q000633:toxicity*
D011500:Protein Synthesis Inhibitors; D012717:Sesquiterpenes; D002244:Carbon; D013605:T-2 Toxin