Inactivation of aflatoxin B1 by using the synergistic effect of hydrogen peroxide and gamma radiation.
Inactivation of aflatoxin B1 was studied by using gamma radiation and hydrogen peroxide. A 100-krad dose of gamma radiation was sufficient to inactivate 50 micrograms of aflatoxin B1 in the presence of 5% hydrogen peroxide, and 400 krad was required for total degradation of 100 micrograms of aflatoxin in the same system. Degradation of aflatoxin B1 was confirmed by high-pressure liquid chromatographic and thin-layer chromatographic analysis. Ames microsomal mutagenicity test showed loss of aflatoxin activity. This method of detoxification also reduces the toxin levels effectively in artificially contaminated groundnuts.
Patel
U D
UD
Department of Microbiology, Faculty of Science, M.S. University of Baroda, India.
Govindarajan
P
P
Dave
P J
PJ
D016604:Aflatoxin B1; D000348:Aflatoxins / Q000037:antagonists & inhibitors / Q000494:pharmacology / Q000528:radiation effects*; D000818:Animals; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008862:Microsomes, Liver / Q000378:metabolism; D009152:Mutagenicity Tests; D012486:Salmonella typhimurium / Q000187:drug effects
D000348:Aflatoxins; D016604:Aflatoxin B1; D006861:Hydrogen Peroxide