title: Measurement of glomerular filtration rate in children with insulin-dependent diabetes mellitus.
abstract: 51Cr-EDTA, inulin and creatinine clearances were measured simultaneously in 11 diabetic children and 12 healthy young adults. The clearances in all individuals were 115 +/- 24 ml/min per 1.73 m2SA for 51Cr-EDTA, 118 +/- 25 ml/min per 1.73 m2SA for inulin and 157 +/- 35 ml/min per 1.73 m2SA for creatinine. Values were higher in the diabetic children but the differences were not significant. 51Cr-EDTA clearance significantly underestimated that of inulin by a mean of -7.4 +/- 2.5 (SEM) ml/min per 1.73 m2SA (p less than 0.01) and creatinine clearance significantly overestimated that of inulin by 37.6 +/- 3.3 (SEM) ml/min per 1.73 m2SA (p less than 0.0001). Similarly, the mean ratio of 51Cr-EDTA to inulin clearance was 0.94 (95% CI 0.90-0.98) and that of creatinine to inulin clearance was 1.32 (95% CI 1.27-1.37); the differences between diabetics and controls were not significant. Correlation coefficients were 0.93 between 51Cr-EDTA and inulin clearances, and 0.95 between inulin and creatinine clearances. The pooled coefficient of variation between clearances within an individual was higher with inulin, 10.3 +/- 6.5% (SD), than 51Cr-EDTA, 7.3 +/- 5.1% (p less than 0.001, t test). These results show that 51Cr-EDTA clearance underestimates that of inulin to a similar extent in both diabetic children and healthy controls and creatinine clearance overestimates inulin clearance to a greater but similar extent in both groups. The methodological variation in 51Cr-EDTA measurement is less than with the other 2 methods. Therefore, we recommend the use of the renal clearance of 51Cr-EDTA for the measurement of GFR in diabetic children.
authors.affiliation: Department of Paediatric Nephrology, Institute of Child Health, London, UK.
authors.forename: D M
authors.initials: DM
authors.lastname: Gibb
authors.forename: N R
authors.initials: NR
authors.lastname: Dalton
authors.forename: M T
authors.initials: MT
authors.lastname: Barratt
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002860:Chromium Radioisotopes; D003404:Creatinine / Q000652:urine; D003922:Diabetes Mellitus, Type 1 / Q000503:physiopathology*; D004492:Edetic Acid / Q000652:urine; D005260:Female; D005919:Glomerular Filtration Rate; D006801:Humans; D007444:Inulin / Q000652:urine; D008297:Male; D008722:Methods
chemical_list: D002860:Chromium Radioisotopes; D007444:Inulin; D004492:Edetic Acid; D003404:Creatinine