title: Regulation of carbon monoxide dehydrogenase and hydrogenase in Rhodospirillum rubrum: effects of CO and oxygen on synthesis and activity.
abstract: Exposure of the photosynthetic bacterium Rhodospirillum rubrum to carbon monoxide led to increased carbon monoxide dehydrogenase and hydrogenase activities due to de novo protein synthesis of both enzymes. Two-dimensional gels of [35S]methionine-pulse-labeled cells showed that induction of CO dehydrogenase synthesis was rapidly initiated (less than 5 min upon exposure to CO) and was inhibited by oxygen. Both CO dehydrogenase and the CO-induced hydrogenase were inactivated by oxygen in vivo and in vitro. In contrast to CO dehydrogenase, the CO-induced hydrogenase was 95% inactivated by heating at 70 degrees C for 5 min. Unlike other hydrogenases, this CO-induced hydrogenase was inhibited only 60% by a 100% CO gas phase.
authors.affiliation: Department of Biochemistry, University of Wisconsin, Madison 53706-1569.
authors.forename: D
authors.initials: D
authors.lastname: Bonam
authors.forename: L
authors.initials: L
authors.lastname: Lehman
authors.forename: G P
authors.initials: GP
authors.lastname: Roberts
authors.forename: P W
authors.initials: PW
authors.lastname: Ludden
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000378:metabolism*; D002248:Carbon Monoxide / Q000502:physiology*; D002701:Chloramphenicol / Q000494:pharmacology; D015180:Electrophoresis, Gel, Two-Dimensional; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000528:radiation effects; D006358:Hot Temperature; D006859:Hydrogen / Q000096:biosynthesis; D006864:Hydrogenase / Q000378:metabolism; D008027:Light; D009097:Multienzyme Complexes; D010100:Oxygen / Q000494:pharmacology*; D012247:Rhodospirillum rubrum / Q000502:physiology*; D012293:Rifampin / Q000494:pharmacology
chemical_list: D009097:Multienzyme Complexes; D002701:Chloramphenicol; D002248:Carbon Monoxide; D006859:Hydrogen; D006864:Hydrogenase; D000445:Aldehyde Oxidoreductases; C019823:carbon monoxide dehydrogenase; D010100:Oxygen; D012293:Rifampin