Carbonic anhydrase II is induced in HL-60 cells by 1,25-dihydroxyvitamin D3: a model for osteoclast gene regulation.
Carbonic anhydrase II (CA II) generates the H+ required for osteoclast-mediated bone resorption in humans. We have developed the human promyelocytic cell line HL-60 as a model system with which to study the osteoclast-specific expression of the CA II gene. Treatment of the cell line with 1,25-dihydroxyvitamin D3 resulted in a dramatic de novo induction of CA II at both the protein and mRNA levels. CA II mRNA was also induced to a lesser extent by 12-O-tetradecanoyl phorbol 13-acetate. Treatment with dimethyl sulfoxide did not increase CA II mRNA. These findings indicate that the HL-60 cell line will be a useful model system to study the osteoclast-specific expression of the CA II gene.
Hillstrom Shapiro
L
L
Department of Human Genetics, University of Michigan Medical School, Ann Arbor 48109-0618.
Venta
P J
PJ
Yu
Y S
YS
Tashian
R E
RE
D015152:Blotting, Northern; D015153:Blotting, Western; D002117:Calcitriol / Q000494:pharmacology*; D002256:Carbonic Anhydrases / Q000096:biosynthesis*; D002460:Cell Line; D004588:Electrophoresis, Cellulose Acetate; D004790:Enzyme Induction; D005786:Gene Expression Regulation; D006801:Humans; D010010:Osteoclasts; D012333:RNA, Messenger / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D012333:RNA, Messenger; D002256:Carbonic Anhydrases; D002117:Calcitriol; D013755:Tetradecanoylphorbol Acetate