Sequence and structure of the Drosophila melanogaster ovarian tumor gene and generation of an antibody specific for the ovarian tumor protein.
Sequencing cDNA and genomic DNA from the ovarian tumor gene revealed a gene with seven introns spanning 4.5 kilobases. The proline-rich, hydrophilic otu protein is novel. An antibody prepared to a beta-gal-otu fusion protein recognized a 110-kilodalton ovarian protein which was altered in the ovaries of otu gene mutants.
Steinhauer
W R
WR
Department of Biochemistry, University of North Carolina, Chapel Hill 27599.
Walsh
R C
RC
Kalfayan
L J
LJ
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex / Q000032:analysis; D001483:Base Sequence; D003001:Cloning, Molecular; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D005091:Exons; D005260:Female; D015723:Gene Library; D007301:Insect Hormones; D007438:Introns; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009154:Mutation; D009857:Oncogenes; D010051:Ovarian Neoplasms / Q000235:genetics*; D015183:Restriction Mapping
D000906:Antibodies; D000936:Antigen-Antibody Complex; D029721:Drosophila Proteins; D007301:Insect Hormones; C061992:otu protein, Drosophila