title: Golf: an olfactory neuron specific-G protein involved in odorant signal transduction.
abstract: Biochemical and electrophysiological studies suggest that odorants induce responses in olfactory sensory neurons via an adenylate cyclase cascade mediated by a G protein. An olfactory-specific guanosine triphosphate (GTP)-binding protein alpha subunit has now been characterized and evidence is presented suggesting that this G protein, termed Golf, mediates olfaction. Messenger RNA that encodes Golf alpha is expressed in olfactory neuroephithelium but not in six other tissues tested. Moreover, within the olfactory epithelium, Golf alpha appears to be expressed only by the sensory neurons. Specific antisera were used to localize Golf alpha protein to the sensory apparatus of the receptor neurons. Golf alpha shares extensive amino acid identity (88 percent) with the stimulatory G protein, Gs alpha. The expression of Golf alpha in S49 cyc- kin- cells, a line deficient in endogenous stimulatory G proteins, demonstrates its capacity to stimulate adenylate cyclase in a heterologous system.
authors.affiliation: Howard Hughes Medical Institute, Department of Molecular Biology and Genetic Johns Hopkins School of Medicine, Baltimore, MD 21205.
authors.forename: D T
authors.initials: DT
authors.lastname: Jones
authors.forename: R R
authors.initials: RR
authors.lastname: Reed
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D019204:GTP-Binding Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D005786:Gene Expression Regulation; D015151:Immunoblotting; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D009475:Neurons, Afferent / Q000032:analysis / Q000502:physiology*; D009812:Odorants; D009830:Olfactory Bulb / Q000502:physiology; D009831:Olfactory Mucosa / Q000032:analysis / Q000294:innervation*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D015398:Signal Transduction; D014018:Tissue Distribution; D014162:Transfection
chemical_list: D012333:RNA, Messenger; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases