Regulation of extracellular proteins and alpha-amylase secretion by temperature in Bacillus subtilis.
alpha-Amylase was found to be the main protein secreted by Bacillus subtilis, corresponding to 90, 87 and 60% of total extracellular proteins at 30, 40 and 45 degrees C, respectively. A change in temperature can affect the pattern of proteins secreted as detected by gel electrophoresis. 14C-Leucine incorporation into extracellular proteins and their proportion at the end of the growth phase was higher at 30 degrees C than that at 40 or 45 degrees C. The effect of temperature on alpha-amylase synthesis as determined by its enzymic activity and on the extracellular protein synthesis followed a similar pattern.
Hao
J
J
Department of Enzyme Engineering, Czechoslovak Academy of Sciences, Prague.
Pazlarová
J
J
Strnadová
M
M
Chaloupka
J
J
D001412:Bacillus subtilis / Q000201:enzymology* / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D007930:Leucine / Q000378:metabolism; D013696:Temperature; D000516:alpha-Amylases / Q000096:biosynthesis* / Q000378:metabolism
D001426:Bacterial Proteins; D000516:alpha-Amylases; D007930:Leucine