Granuloma formation in Schistosoma japonicum infected nude mice: the effects of reconstitution with L3T4+ or Lyt2+ splenic cells.
The role of L3T4+ and Lyt2+ lymphocytes in the formation and modulation of granulomas around Schistosoma japonicum eggs was examined in athymic mice. Nude C57BL/6 mice infected with S. japonicum miracidia were compared to nude mice given normal spleen cells depleted of L3T4+ or Lyt2+ cells prior to infection and to intact mice. Nude mice formed small granulomas that were poor in eosinophils and connective tissue. Nude mice reconstituted with cells enriched for L3T4+ or Lyt2+ cells formed granulomas similar in size to those in intact mice, but granulomas in mice given Lyt2+ cells contained few eosinophils and had significantly less collagen than did granulomas in mice given L3T4+ cells. The same was true at 10 weeks. Mice reconstituted with null cells were examined at 7 weeks and formed granulomas similar to those in mice given Lyt2+ cells. By 10 weeks after infection, granulomas had decreased to the same minute size in all groups of mice. Fibrosis increased at weeks 7-10 in all groups of mice.
Cheever
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AW
Laboratory of Parasitic Diseases, National Institutes of Health, Bethesda, Maryland 20892.
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Duvall
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D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis; D000950:Antigens, Ly / Q000032:analysis; D004804:Eosinophils; D006099:Granuloma / Q000276:immunology* / Q000473:pathology; D008103:Liver Cirrhosis / Q000473:pathology; D008109:Liver Diseases, Parasitic / Q000276:immunology* / Q000473:pathology; D008214:Lymphocytes; D008264:Macrophages; D051379:Mice; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D010270:Parasite Egg Count; D012549:Schistosoma japonicum / Q000276:immunology; D012554:Schistosomiasis japonica / Q000276:immunology* / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology*
D000909:Antibodies, Helminth; D000950:Antigens, Ly