An antiserum against amyloid beta-protein precursor detects a unique peptide in Alzheimer brain.
An antiserum was raised against an amino acid sequence predicted from the DNA sequence of amyloid beta-protein precursor (ABPP), and it was then affinity-purified. This affinity-purified antibody (anti-GID) intensely stained neurons and dystrophic neurites in plaques of Alzheimer's disease (AD) patients, but marginally stained neurons of age-matched normal individuals. Anti-GID antibody detected a series of protein bands with a molecular weight centered at 100,000 and a second band at 55,000 on a blot of the human brain particulate fraction. It also stained a set of bands with a molecular weight around 95,000 and a doublet of Mr 16,000 in the soluble fraction. A band at Mr 35,000 was detected in the soluble fraction prepared from brain tissue of AD patients but not from control brain tissue. A strong immunostaining of AD sections with anti-GID and the presence of a Mr 35,000 band unique to AD might reflect an altered processing of ABPP in AD brains.
Cole
G
G
Department of Neurosciences, University of California, San Diego, School of Medicine, La Jolla 92093.
Masliah
E
E
Huynh
T V
TV
DeTeresa
R
R
Terry
R D
RD
Okuda
C
C
Saitoh
T
T
D000544:Alzheimer Disease / Q000378:metabolism* / Q000473:pathology; D000682:Amyloid / Q000378:metabolism*; D016564:Amyloid beta-Protein Precursor; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007106:Immune Sera; D007150:Immunohistochemistry; D008970:Molecular Weight; D011498:Protein Precursors / Q000378:metabolism*
D000682:Amyloid; D016564:Amyloid beta-Protein Precursor; D007106:Immune Sera; D011498:Protein Precursors