Serum levels of soluble CD8 are increased in patients with B chronic lymphocytic leukemia.
Serum levels of the soluble form of the CD8 (s-CD8) antigen were evaluated in the peripheral blood of 44 patients with B-CLL, using an enzyme-linked immunosorbent assay, and were correlated with clinical features and relevant hematological and immunological data. Increased values were observed with respect to normal age-matched controls (mean +/- S.E.M. 603 U/ml +/- 81 vs. 315 U/ml +/- 31, respectively; P less than 0.0001). This increase was observed in all stages of the disease, excluding stage 0 (mean 277 U/ml +/- 45). A general trend pointing to lower values overall in patients with less invasive disease was observed. In fact, the rank correlation test showed that the serum levels of s-CD8 correlate significantly with the WBC counts, the CD4/CD8 ratio, and also with the levels of serum immunoglobulins. On the contrary, no correlation was observed between s-CD8 levels and the absolute number of circulating CD8+ cells in individual cases. Therefore, the increase of s-CD8 is unlikely to be a mere expression of the increase of the CD8 cell number, but seems related to an increase of the activation phenomena involving the CD8+ T cell subset.
Semenzato
G
G
Padua University School of Medicine, Department of Clinical Medicine, Italy.
Siviero
F
F
Vinante
F
F
Zambello
R
R
Trentin
L
L
Agostini
C
C
Adami
F
F
Vincenzi
C
C
Zanotti
R
R
Zuppini
B
B
D000945:Antigens, Differentiation, T-Lymphocyte / Q000097:blood*; D016827:CD8 Antigens; D005260:Female; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D012995:Solubility
D000945:Antigens, Differentiation, T-Lymphocyte; D016827:CD8 Antigens