Epidermal growth factor induces the expression of its receptor gene in human gastric carcinoma cell line TMK-1.
To ascertain the possible autocrine pathway in the growth promotion of gastric carcinomas, a study was made on the effects of exogenous human epidermal growth factor (hEGF) on the expression of mRNA for EGF, transforming growth factor-a (TGF-a), EGF receptor, FOS and MYC genes by TMK-1 cells. Exogenous hEGF increased FOS and MYC mRNA levels 30 min and 1 h after the treatment, respectively. TMK-1 cells accumulated the mRNA for EGF receptor about 7- to 8-fold by 3 h after treatment. Expressions of mRNA for EGF and TGF-a genes were detected, but the amounts of the mRNA of these genes in TMK-1 cells were not altered after the treatment.
Yoshida
K
K
First Department of Pathology, Hiroshima University School of Medicine.
Takanashi
A
A
Kyo
E
E
Ito
M
M
Ito
H
H
Niimoto
M
M
Hattori
T
T
Tahara
E
E
D002460:Cell Line; D004815:Epidermal Growth Factor / Q000235:genetics / Q000494:pharmacology*; D066246:ErbB Receptors / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D005796:Genes / Q000187:drug effects*; D006801:Humans; D007700:Kinetics; D011519:Proto-Oncogenes / Q000187:drug effects*; D012333:RNA, Messenger / Q000235:genetics; D013274:Stomach Neoplasms; D014158:Transcription, Genetic / Q000187:drug effects*; D015291:Transforming Growth Factors / Q000235:genetics; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism*
D012333:RNA, Messenger; D004815:Epidermal Growth Factor; D015291:Transforming Growth Factors; D066246:ErbB Receptors