Multiple GF-1 binding sites flank the erythroid specific transcription unit of the human carbonic anhydrase I gene.
Six potential GF-1 sites which bind an erythroid factor are present in the 5' and 3' regions flanking the erythroid-specific transcription unit of the human carbonic anhydrase I (HCAI) gene. When two of these sites are placed upstream of a minimal eukaryotic promoter they confer up-regulated expression in erythroid over non-erythroid cells. The presence of the erythroid factor in TPA-treated HEL cells in which the level of HCAI transcript has greatly decreased and in non-HCAI-expressing K562 cells suggests that in these cases the presence of the factor is not sufficient for HCAI expression.
Brady
H J
HJ
Department of Biochemistry, University College London, England.
Sowden
J C
JC
Edwards
M
M
Lowe
N
N
Butterworth
P H
PH
D001483:Base Sequence; D001665:Binding Sites; D002256:Carbonic Anhydrases / Q000235:genetics*; D002460:Cell Line; D004912:Erythrocytes / Q000502:physiology*; D005786:Gene Expression Regulation; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D014157:Transcription Factors / Q000502:physiology*; D014158:Transcription, Genetic
D014157:Transcription Factors; D002256:Carbonic Anhydrases