title: Arachidonic acid metabolism by perfused ram testis.
abstract: 1. Arachidonic acid was metabolized by lipoxygenase and prostaglandin synthetase enzymes systems in the perfused ram testis. 2. The major product of the prostaglandin synthetase was 6-keto-PGF1 alpha (6KF). 3. Addition of testosterone resulted in a significant increase in the 6KF. 4. Arachidonic acid (AA) as well as testosterone penetrated the perfused testis. 5. Both 15-HPETE and 15-HETE, the products of the 15-lipoxygenase enzyme, were detected. 6. Addition of 0.1% BSA changed the pattern of the oxidized arachidonic acid metabolism.
authors.affiliation: Department of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel.
authors.forename: I
authors.initials: I
authors.lastname: Schön
authors.forename: Y
authors.initials: Y
authors.lastname: Sofer
authors.forename: M
authors.initials: M
authors.lastname: Cojacaru
authors.forename: S
authors.initials: S
authors.lastname: Grossman
mesh_terms: D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis; D066298:In Vitro Techniques; D015289:Leukotrienes / Q000096:biosynthesis; D008054:Lipid Peroxides / Q000096:biosynthesis; D008084:Lipoxygenase / Q000378:metabolism; D008297:Male; D010084:Oxidation-Reduction; D010477:Perfusion; D011453:Prostaglandins / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D012756:Sheep; D013737:Testis / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D013739:Testosterone / Q000494:pharmacology
chemical_list: D001095:Arachidonic Acids; D006893:Hydroxyeicosatetraenoic Acids; D015289:Leukotrienes; D008054:Lipid Peroxides; D011453:Prostaglandins; D012710:Serum Albumin, Bovine; D016718:Arachidonic Acid; D013739:Testosterone; C025086:15-hydroperoxy-5,8,11,13-eicosatetraenoic acid; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid; D008084:Lipoxygenase