Analysis of a common-antigen lipopolysaccharide from Pseudomonas aeruginosa.
Lipopolysaccharide isolated from Pseudomonas aeruginosa PAO1 (O5 serotype) was separated into two antigenically distinct fractions. A minor fraction, containing shorter polysaccharide chains, reacted with a monoclonal antibody to a P. aeruginosa common antigen but did not react with antibodies specific to O5-serotype lipopolysaccharide. In contrast, fractions containing long polysaccharide chains reacted only with the O5-specific monoclonal antibodies. The shorter, common-antigen fraction lacked phosphate and contained stoichiometric amounts of sulfate, and the fatty acid composition of this fraction was similar to that of the O-antigen-specific fraction. The lipid A derived from the serotype-specific lipopolysaccharide cross-reacted with monoclonal antibodies against lipid A from Escherichia coli, while the lipid A derived from the common antigen did not react. We propose that many serotypes of P. aeruginosa produce two chemically and antigenically distinct lipopolysaccharide molecules, one of which is a common antigen with a short polysaccharide and a unique core-lipid A structure.
Rivera
M
M
Department of Biochemistry, Michigan State University, East Lansing 48824.
McGroarty
E J
EJ
D000942:Antigens, Bacterial / Q000302:isolation & purification*; D015153:Blotting, Western; D002241:Carbohydrates / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D008033:Limulus Test; D008070:Lipopolysaccharides / Q000302:isolation & purification*; D011550:Pseudomonas aeruginosa / Q000032:analysis* / Q000276:immunology
D000942:Antigens, Bacterial; D002241:Carbohydrates; D005227:Fatty Acids; D008070:Lipopolysaccharides