Coamplification of the L-myc and N-myc oncogenes in a neuroblastoma cell line.
The L-myc, N-myc and c-myc genes are members of the myc oncogene family. In particular, L-myc is novel, and amplification of L-myc is still unknown except in small cell lung carcinoma. We examined L-myc amplification in 30 human neuroblastomas using Southern blot hybridization, and found that the L-myc gene was amplified approximately 5-fold in GOTO, a human neuroblastoma cell line. The N-myc gene was also amplified approximately 60-fold and furthermore, over-expression of L-myc and N-myc genes was observed in this cell line. In this report, we describe the coamplification of the myc gene family in the GOTO neuroblastoma cell line.
Jinbo
T
T
Department of Pediatric Surgery, University of Tsukuba, Ibaraki.
Iwamura
Y
Y
Kaneko
M
M
Sawaguchi
S
S
D015139:Blotting, Southern; D005784:Gene Amplification; D006801:Humans; D009447:Neuroblastoma / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc; D014407:Tumor Cells, Cultured
D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc