title: Characterization of murine thymocyte subpopulations reacting with Dolichos bifloris agglutinin.
abstract: Reactivity of murine thymocytes with the Dolichos bifloris agglutinin (DBA) was examined using flow cytometry. This agglutinin, which has nominal specificity for alpha-linked N-acetyl-D-galactosamine residues, labels 1-4% of unfractionated BALB/c thymocytes. Among thymocyte subpopulations identified on the basis of CD4/CD8 labeling, DBA bound to a substantial percentage of the CD4-8- thymocyte subpopulation. In addition, small populations of thymocytes expressing CD4 and/or CD8 were also labeled with DBA, but with less intensity then seen on many CD4-8- cells. Within the CD4-8- thymocyte population, labeling with DBA was positively correlated with reactivity with J11d antibody, the expression of IL-2 receptor and high levels of the homing receptor molecule, MEL-14. Reactivity with DBA was negatively correlated with the expression of Lyt-1, V beta 8 TCR determinants, and CD3.
authors.affiliation: Department of Biological Structure, University of Washington, Seattle 98195.
authors.forename: K
authors.initials: K
authors.lastname: Scofield
authors.forename: Z J
authors.initials: ZJ
authors.lastname: Yan
authors.forename: A G
authors.initials: AG
authors.lastname: Farr
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis*; D016827:CD8 Antigens; D005434:Flow Cytometry; D037102:Lectins / Q000494:pharmacology*; D008214:Lymphocytes / Q000145:classification*; D051379:Mice; D008807:Mice, Inbred BALB C; D037121:Plant Lectins; D015375:Receptors, Interleukin-2 / Q000032:analysis; D013950:Thymus Gland / Q000166:cytology*
chemical_list: D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D016827:CD8 Antigens; D037102:Lectins; D037121:Plant Lectins; D015375:Receptors, Interleukin-2; C036554:dolichos biflorus agglutinin