Thirty compounds tested in the Drosophila wing spot test.
The Drosophila wing somatic mutation and recombination test (SMART) was evaluated for its suitability in genotoxicity screening by testing 30 chemicals. Of the 2 crosses used, the mwh-flr3 cross turned out to be more convenient than the previously used mwh-flr cross. Based on the experience gained with both acute exposures and chronic exposures of different duration, we suggest that the optimal strategy in genotoxicity screening is to start with chronic exposure of 3-day-old larvae for 48 h (that is, until pupation). Only for unstable compounds and very volatile compounds and gases are acute treatments, including inhalation, recommended. In general, a qualitative evaluation of the genotoxicity of a compound in the wing assay is possible with as few as 1-2 different exposure concentrations. A more quantitative evaluation of genotoxicity, based upon dose-response data, can often be achieved with as few as 3-4 concentrations. The results reported here were obtained in 2 different laboratories, demonstrating that the wing spot test is easily transferable to other laboratories. The experience gained indicates that the assay has now been developed to an extent that a coordinated international comparative validation study is desirable.
Graf
U
U
Institute of Toxicology, Swiss Federal Institute of Technology, Schwerzenbach, Switzerland.
Frei
H
H
Kägi
A
A
Katz
A J
AJ
Würgler
F E
FE
D000818:Animals; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics*; D005854:Germ Cells / Q000187:drug effects; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000008:administration & dosage; D013223:Statistics as Topic; D014921:Wings, Animal
D009153:Mutagens