Transformation of Bacillus cereus vegetative cells by electroporation.
Transformation of untreated vegetative cells of Bacillus cereus 569 with plasmid pC194 (1.8 megadaltons) by high-voltage electroporation resulted in a maximum of 2 x 10(-5) transformants per viable cell. Transformation of a 130-megadalton plasmid occurred at a comparable frequency. The method was simple, rapid, and yielded transformant colonies in 14 to 24 h. Transformation was obtained with unpurified total plasmid DNA.
Belliveau
B H
BH
Department of Environmental Biology, University of Guelph, Ontario, Canada.
Trevors
J T
JT
D001409:Bacillus cereus / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D010957:Plasmids; D014170:Transformation, Genetic
D004269:DNA, Bacterial