Sensitive determination of circulating anodic antigen in Schistosoma mansoni infected individuals by an enzyme-linked immunosorbent assay using monoclonal antibodies.
From a panel of mouse monoclonal antibodies reactive with a repeating epitope of the schistosome circulating anodic antigen, an IgG1 monoclonal antibody was selected. This monoclonal antibody was applied in a sandwich enzyme-linked immunosorbent assay as capture antibody and as alkaline phosphatase labeled conjugate. This assay allowed a sensitive quantitation of circulating anodic antigen in serum samples of infected individuals, detecting less than 1 ng antigen/ml serum. In Schistosoma mansoni infected individuals from Zaire, the level of antigen in serum correlated with fecal egg output. The lower detection level of the immunoassay corresponded to a level of about 10 eggs/gm feces.
Deelder
A M
AM
Laboratory of Parasitology, State University of Leiden, The Netherlands.
De Jonge
N
N
Boerman
O C
OC
Fillié
Y E
YE
Hilberath
G W
GW
Rotmans
J P
JP
Gerritse
M J
MJ
Schut
D W
DW
D000818:Animals; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth / Q000032:analysis*; D015023:Democratic Republic of the Congo; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D010270:Parasite Egg Count / Q000379:methods; D012550:Schistosoma mansoni / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000175:diagnosis; D012680:Sensitivity and Specificity; D012698:Serologic Tests; D014238:Trichloroacetic Acid
D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D014238:Trichloroacetic Acid