title: Postnatal development and possible ligand function of the CNS-specific membrane glycoprotein CNSgp130.
abstract: CNSgp 130 is a CNS-specific membrane glycoprotein present in large amounts in the adult mammalian CNS. Using immunohistological techniques, we demonstrated that CNSgp130 is not detectable in the rat cerebellum at birth, and does not appear in the cerebellum until the tenth day of postnatal life. It is expressed first in the white matter of the cerebellar folia, and subsequently (by day 14) it is expressed also in the molecular layer. Expression in the granular layer is not seen until the 18th day of postnatal life, by which time the adult pattern of expression is established. CNSgp130 is also not detectable in the cerebrum at birth. However, it is expressed weakly but diffusely in the cerebrum by the fourth day of life. By the 10th day, there is strong expression in the cerebrum, in marked contrast to its virtual absence from the cerebellum at this stage. By quantitative absorption analysis, CNSgp 130 was undetectable on the day of birth, and increased steadily to 80% of adult values by the 22nd day of postnatal life. Binding studies with pure CNSgp130 demonstrated a Pronase-sensitive ligand in adult chicken brain. This ligand was absent from neonatal rat brain and non-CNS tissues.
authors.affiliation: Blond McIndoe Centre, Queen Victoria Hospital, East Grinstead, Sussex, England.
authors.forename: B F
authors.initials: BF
authors.lastname: Flanagan
authors.forename: J W
authors.initials: JW
authors.lastname: Fabre
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D001921:Brain / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D002241:Carbohydrates / Q000494:pharmacology; D015816:Cell Adhesion Molecules, Neuronal / Q000378:metabolism*; D002531:Cerebellum / Q000254:growth & development / Q000378:metabolism; D002645:Chickens; D058966:Contactins; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D019006:Neural Cell Adhesion Molecules; D011402:Pronase / Q000494:pharmacology; D051381:Rats
chemical_list: D002241:Carbohydrates; D015816:Cell Adhesion Molecules, Neuronal; D058966:Contactins; D019006:Neural Cell Adhesion Molecules; D004533:Egtazic Acid; D004492:Edetic Acid; D011402:Pronase