title: Cholera and pertussis toxins amplify prostacyclin synthesis in aortic smooth muscle cells.
abstract: Pretreatment of bovine aortic smooth muscle cells in culture with pertussis toxin (PT) or cholera toxin (CT) potentiated the synthesis of prostacyclin (PGI2) induced by 5-hydroxytryptamine (5-HT) and phorbol-12-myristate, 13-acetate (PMA). The production of PGI2 by explants from the bovine aortic media was also synergistically stimulated by 5-HT and CT, whereas PT was inactive. These data are consistent with the hypothesis that guanosine 5'-triphosphate binding proteins are directly involved in the control of phospholipases which release free arachidonic acid for prostaglandin synthesis.
authors.affiliation: Institute of Interdisciplinary Research, School of Medicine, Université Libre de Bruxelles, Belgium.
authors.forename: D
authors.initials: D
authors.lastname: Demolle
authors.forename: J M
authors.initials: JM
authors.lastname: Boeynaems
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects / Q000378:metabolism; D002417:Cattle; D002772:Cholera Toxin / Q000494:pharmacology*; D011464:Epoprostenol / Q000096:biosynthesis*; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism*; D037342:Pertussis Toxin; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
chemical_list: D010566:Virulence Factors, Bordetella; D002772:Cholera Toxin; D011464:Epoprostenol; D037342:Pertussis Toxin