Noninactivation of a selectable human X-linked gene that complements a murine temperature-sensitive cell cycle defect.
The human gene A1S9T, which complements the temperature-sensitive cell-cycle defect in the murine cell line tsA1S9 and which has previously been assigned to the X-chromosome short arm, is expressed from the inactive X chromosome in human/tsA1S9 somatic cell hybrids grown at the nonpermissive temperature. The Y chromosome cannot complement the defect; thus, unlike at least two other noninactivated X loci, A1S9T has no functional Y-linked homologue. As A1S9T is readily selectable in somatic cell hybrids with the tsA1S9 mouse line, this marker should be useful in isolating somatic cell hybrids containing inactive X chromosomes, or abnormal X's (active or inactive) retaining the short arm.
Brown
C J
CJ
Department of Medical Genetics, University of Toronto.
Willard
H F
HF
D000818:Animals; D002453:Cell Cycle / Q000235:genetics*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology*; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics* / Q000378:metabolism; D007621:Karyotyping; D008745:Methylation; D051379:Mice; D012867:Skin / Q000166:cytology; D013696:Temperature; D014960:X Chromosome
D005954:Glucosephosphate Dehydrogenase; D007041:Hypoxanthine Phosphoribosyltransferase