title: Differential response of human and rodent cell lines to chemical inhibition of the repair of potentially lethal damage.
abstract: We have examined the effects of several classes of metabolic inhibitors on the repair of potentially lethal damage in density-inhibited cultures of two rodent and two human cell systems which differ in their growth characteristics. Aphidicolin, 1-beta-D-arabinofuranosylcytosine (ara-C) and hydroxyurea showed no effect on PLD repair, whereas the effects of 9-beta-D-arabinofuranosyladenine (ara-A) and 3-aminobenzamide (3-AB) were cell line dependent. For example, 3-AB suppressed PLD repair almost completely in CHO cells, but showed no inhibitory effects in human diploid fibroblasts. These results indicate that inhibitors of DNA replication and poly(ADP-ribose) synthesis are not efficient inhibitors of cellular recovery in irradiated cells and, moreover, that such effects may be cell line dependent.
authors.affiliation: Laboratory of Radiobiology, Harvard School of Public Health, Boston, MA 02115.
authors.forename: J B
authors.initials: JB
authors.lastname: Little
authors.forename: A M
authors.initials: AM
authors.lastname: Ueno
authors.forename: W K
authors.initials: WK
authors.lastname: Dahlberg
mesh_terms: D000246:Adenosine Diphosphate Ribose / Q000096:biosynthesis; D000818:Animals; D016590:Aphidicolin; D001549:Benzamides / Q000494:pharmacology; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D003561:Cytarabine / Q000494:pharmacology; D004257:DNA Polymerase II / Q000037:antagonists & inhibitors; D004260:DNA Repair / Q000187:drug effects*; D004224:Diterpenes / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology; D051379:Mice; D014740:Vidarabine / Q000494:pharmacology
chemical_list: D001549:Benzamides; D004224:Diterpenes; D003561:Cytarabine; D000246:Adenosine Diphosphate Ribose; D016590:Aphidicolin; C025160:3-aminobenzamide; D004257:DNA Polymerase II; D014740:Vidarabine; D006918:Hydroxyurea