title: Lytic effect of Pseudomonas aeruginosa elastase on gram-positive and -negative bacteria.
abstract: Elastase of Pseudomonas aeruginosa P3 caused lysis of freshly grown cells of Gram-positive (Staphylococcus aureus and S. epidermidis) and Gram-negative (Proteus vulgaris and Salmonella typhi) bacteria. Heat killed and lyophilized Gram-negative bacteria showed higher sensitivity to elastase. Both Gram-negative and Gram-positive bacteria were lysed maximally by elastase at pH 8.0. At this pH, the activity of elastase was maximal in Tris-HCl and glycine-NaOH buffers followed by Tris-maleate and Cacodylate buffers.
authors.affiliation: Department of Microbiology, Panjab University, India.
authors.forename: M
authors.initials: M
authors.lastname: Kaur
authors.forename: M
authors.initials: M
authors.lastname: Gupta
authors.forename: K K
authors.initials: KK
authors.lastname: Tripathi
authors.forename: K G
authors.initials: KG
authors.lastname: Gupta
mesh_terms: D001433:Bacteriolysis; D005612:Freeze Drying; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D010196:Pancreatic Elastase / Q000302:isolation & purification / Q000494:pharmacology / Q000502:physiology*; D011550:Pseudomonas aeruginosa / Q000201:enzymology*
chemical_list: D010196:Pancreatic Elastase