title: Structure of full-length cDNA coding for sulfatide activator, a Co-beta-glucosidase and two other homologous proteins: two alternate forms of the sulfatide activator.
abstract: Full-length cDNA clones have been isolated for an mRNA which codes for four different but homologous proteins--a sulfatide activator protein, a co-beta-clucosidase, and two other proteins of similar structures. The primary structure as deduced from the nucleotide sequence is highly homologous to the precursor of the rat Sertoli cell sulfated glycoprotein 1. The full-length clone was 2,734-bp long, starting from 8 bases above the initiator ATG and terminating with a poly A tail. The nucleotide sequence confirmed an earlier prediction based on the amino acid sequence that a previously published sequence contained errors. On the other hand, the amino acid sequence now closely agrees with the recent revised sequence published by the same group except for several amino acids near the N-terminus. Two alternate forms of the sulfatide activator were detected, differing from each other by the presence or absence of 3-amino acid insertion.
authors.affiliation: Biological Sciences Research Center, University of North Carolina School of Medicine, Chapel Hill 27599.
authors.forename: T
authors.initials: T
authors.lastname: Nakano
authors.forename: K
authors.initials: K
authors.lastname: Sandhoff
authors.forename: J
authors.initials: J
authors.lastname: Stümper
authors.forename: H
authors.initials: H
authors.lastname: Christomanou
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis* / Q000235:genetics; D005959:Glucosidases / Q000032:analysis*; D006023:Glycoproteins / Q000032:analysis*; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000032:analysis / Q000235:genetics; D049231:Saposins; D049128:Sphingolipid Activator Proteins; D013462:Sulfur Radioisotopes; D001617:beta-Glucosidase / Q000032:analysis* / Q000235:genetics
chemical_list: D006023:Glycoproteins; D011498:Protein Precursors; C483347:Psap protein, rat; D049231:Saposins; D049128:Sphingolipid Activator Proteins; D013462:Sulfur Radioisotopes; D004247:DNA; D005959:Glucosidases; D001617:beta-Glucosidase