Dietary propylthiouracil and 17 beta-estradiol benzoate implant effects on liver carbohydrate and lipid metabolism in chicks.
1. Forty-eight chicks (21 days old) were implanted (+/- 50 mg 17-beta estradiol; EBI) and fed diets containing +/- 0.1% propylthiouracil (PTU) for 7 days to determine the role of gonadal hormones in the regulation of energy metabolism in the hypothyroid chick. 2. In vitro lipogenesis (IVL) and glucose production (NGP) were measured in liver explants. 3. Liver glycogen (GLY) metabolism was studied in particulate fractions (50,000 x g) of liver. 4. Glycogen synthetase activity (GLYSYN) was assayed in the presence of glucose-6-phosphate (G-6P; 0, 0.15 and 10 mM) to determine activation states. 5. PTU and EBI increased (P less than 0.05) both GLY concentration and GLYSYN activity in chicks but, did not increase G-6P activity ratios or the in vitro activation of GLYSYN. 6. Both PTU and EBI increased (P less than 0.05) IVL and NGP. 7. Estradiol magnifies the effects of PTU in chicks suggesting an interaction between thyroid status and gonadal function.
Rosebrough
R
R
USDA-Agricultural Research Service, Beltsville Agricultural Research Center, MD 20705.
McMurtry
J
J
Steele
N
N
D000818:Animals; D050260:Carbohydrate Metabolism; D002645:Chickens / Q000378:metabolism*; D004032:Diet; D004343:Drug Implants; D004958:Estradiol / Q000008:administration & dosage / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D006006:Glycogen Synthase / Q000378:metabolism; D050356:Lipid Metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D011441:Propylthiouracil / Q000008:administration & dosage / Q000494:pharmacology*
D004343:Drug Implants; D004958:Estradiol; D011441:Propylthiouracil; D006003:Glycogen; D006006:Glycogen Synthase; D005947:Glucose