Bovine transforming growth factor beta. Purification and characterization.
Polypeptide growth factor isolated from bovine platelets was classified on the basis of its physico-chemical and biological properties to be transforming growth factor type beta (TGF beta). This growth factor was completely purified from platelet lysate by acid ethanol extraction, Bio-Gel P-60 filtration, ion-exchange chromatography on CM-Sephadex followed by two successive gel filtrations on Bio-Gel P-10 and Sephadex G-100. After the last gel filtration over 35,000-fold purified TGF beta was obtained. The purity of the preparation was confirmed by SDS-polyacrylamide gel electrophoresis, which revealed a single protein band with Mr of 25,000-27,000. Further studies showed that native TGF beta consists of two Mr 13,000 polypeptide chains held together by disulfide bonds.
Popik
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W
Department of Virology, Polish Academy of Sciences, Wrocław.
Inglot
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O
D000818:Animals; D001792:Blood Platelets / Q000737:chemistry; D002417:Cattle; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D011487:Protein Conformation; D016212:Transforming Growth Factor beta / Q000737:chemistry / Q000302:isolation & purification*
D016212:Transforming Growth Factor beta