Modifying role of dietary factors on the mutagenicity of aflatoxin B1: in vitro effect of plant flavonoids.
Eighteen flavonoids have been tested for their ability to inhibit the mutagenicity of aflatoxin B1 (AFB1) towards strains TA100 and TA98 of Salmonella typhimurium provided with a rat liver activation system. These flavonoids belong to 5 different groups: flavone, isoflavone, flavanone, flavanol and flavonol, and many individual members are natural products present in edible portions of a variety of food plants. Several flavonoids exhibited significant inhibitory ability in both strains. Flavonols in general are more active in this regard, while flavanones show a strain-specific response. The flavanol group of compounds did not display any activity. Among the most effective flavonoids are kaempferol, morin, fisetin, biochanin A and the glycoside rutin, all of which exhibit a dose-dependent inhibition pattern. Kaempferol and rutin, in particular, show exceptional activity inasmuch as, on a molar basis, only a 10-fold excess dose of each can inhibit the mutagenic activity of AFB1 in strain TA98 by 50%. The action of flavonoids is possibly mediated through interaction with microsomal activating enzymes. Previous evidence from this laboratory about their inhibitory action on DNA-adduct formation and metabolic activation together with the present results suggests that certain flavonoids, notably polyhydroxylated flavonols, may have potential anticarcinogenic activity against AFB1.
Francis
A R
AR
Biochemistry Division, Bhabha Atomic Research Centre, Bombay, India.
Shetty
T K
TK
Bhattacharya
R K
RK
D016604:Aflatoxin B1; D000348:Aflatoxins / Q000037:antagonists & inhibitors / Q000633:toxicity*; D000818:Animals; D001711:Biotransformation / Q000187:drug effects; D004032:Diet; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000494:pharmacology*; D066298:In Vitro Techniques; D009152:Mutagenicity Tests; D009154:Mutation / Q000187:drug effects*; D010944:Plants; D051381:Rats
D000348:Aflatoxins; D005419:Flavonoids; D016604:Aflatoxin B1