Interaction of cellular factors related to the Jun oncoprotein with the promoter of a replication-dependent hamster histone H3.2 gene.
The promoter region of a replication-dependent histone H3.2 gene contains a putative DNA binding site for the Jun oncoprotein within a 32-nucleotide regulatory domain. The hamster sequence differs by one nucleotide from the AP-1 consensus sequence found in several promoters responsive to phorbol 12-myristate 13-acetate. We have identified the factors interacting with this region as 42- and 45-kDa proteins by DNA affinity chromatography, immunoblotting, and UV crosslinking. These proteins, which are candidates for conferring high-level expression to the histone promoter, share an antigenic epitope with the DNA-binding domain of Jun but diverge from it at the amino terminus. The interaction of these proteins with the promoter of a replication-dependent cellular gene provides evidence that members of the Jun oncoprotein family may play specific roles in the expression of genes essential for progression of the cell cycle.
Sharma
A
A
Department of Biochemistry, University of Southern California School of Medicine, Los Angeles 90033.
Bos
T J
TJ
Pekkala-Flagan
A
A
Vogt
P K
PK
Lee
A S
AS
D000818:Animals; D000906:Antibodies / Q000276:immunology; D001483:Base Sequence; D002846:Chromatography, Affinity; D006224:Cricetinae; D003429:Cross Reactions; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D015342:DNA Probes; D004261:DNA Replication; D003850:Deoxyribonuclease I / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000235:genetics*; D015151:Immunoblotting; D008969:Molecular Sequence Data; D016759:Oncogene Protein p65(gag-jun); D011401:Promoter Regions, Genetic; D012191:Retroviridae Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000276:immunology / Q000378:metabolism
D000906:Antibodies; D015342:DNA Probes; D006657:Histones; D016759:Oncogene Protein p65(gag-jun); D012191:Retroviridae Proteins; D014157:Transcription Factors; D004247:DNA; D003850:Deoxyribonuclease I