Characteristic mode of action of gangliosides in selective modulation of CD4 on human T lymphocytes.
We have explored the possible mechanisms for selective modulation by gangliosides of CD4 on human T lymphocytes and subsequent re-expression of CD4. Indirect immunofluorescence staining with anti-CD4 antibodies revealed newly internalized CD4 in ganglioside-treated cells after membrane permeabilization with 0.1% saponin. Cycloheximide and other metabolic inhibitors did not alter the modulation but inhibited significantly the re-expression of CD4. These results suggest both selective modulation of CD4 by a process of endocytosis and re-expression of CD4 through de novo protein synthesis.
Kawaguchi
T
T
Second Department of Internal Medicine, Kumamoto University Medical School, Japan.
Nakakuma
H
H
Kagimoto
T
T
Shirono
K
K
Horikawa
K
K
Hidaka
M
M
Iwamori
M
M
Nagai
Y
Y
Takatsuki
K
K
D000945:Antigens, Differentiation, T-Lymphocyte; D015153:Blotting, Western; D002462:Cell Membrane / Q000276:immunology; D002463:Cell Membrane Permeability / Q000187:drug effects; D002772:Cholera Toxin; D004305:Dose-Response Relationship, Drug; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D005677:G(M1) Ganglioside / Q000494:pharmacology; D005732:Gangliosides / Q000494:pharmacology*; D006801:Humans; D015458:Leukemia, T-Cell; D012503:Saponins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D013861:Thiocyanates; D014407:Tumor Cells, Cultured
D000945:Antigens, Differentiation, T-Lymphocyte; D005452:Fluoresceins; D005456:Fluorescent Dyes; D005732:Gangliosides; D012503:Saponins; D013861:Thiocyanates; D005677:G(M1) Ganglioside; D002772:Cholera Toxin; D016650:Fluorescein-5-isothiocyanate