Hierarchy of cytotoxic T cell clones. II. Intraclonal triggering of lysis by hapten-specific CTL.
Cells from clones of anti-hapten murine cytotoxic T lymphocytes (CTL) can act as both target and effector cells, but will not lyse members of the same clone. The effect of haptenation on the cytolytic activity of anti-fluorescein (FL) and anti-trinitrophenol (TNP) CTL clones was examined. Treatment of anti-FL clones with fluorescein isothiocyanate or anti-TNP clones with trinitrobenzene sulphonic acid induces these clones to kill in an antigen-independent fashion. Targets killed by the haptenated CTL included syngeneic and allogeneic B lymphocyte blast cells, P815, YAC-1 and in one case human GM 4072 tumor cells. The importance of CD8 and T cell receptor (TCR) occupancy is demonstrated by the ability to block autotriggering by antibody directed against Ly 2 and the TCR. The results demonstrate that effects other than antigen recognition of the target play a role in the final outcome of effector-target cell interactions and provide a mechanism which could lead to autodestruction and immunosuppression particularly in some types of viral infection.
Skinner
M
M
Department of Immunobiology, University of Auckland, New Zealand.
Hartley
L
L
Marbrook
J
J
D000818:Animals; D000937:Antigen-Antibody Reactions; D000950:Antigens, Ly / Q000276:immunology; D002999:Clone Cells / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D019793:Fluorescein; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins / Q000276:immunology / Q000494:pharmacology; D006241:Haptens; D007111:Immunity, Cellular; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D051379:Mice; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D013861:Thiocyanates / Q000276:immunology / Q000494:pharmacology
D000950:Antigens, Ly; D005452:Fluoresceins; D006241:Haptens; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate; D019793:Fluorescein