Macrophage cell lines P388D1 and IC-21 stimulated with gamma interferon fail to inhibit the intracellular growth of Histoplasma capsulatum.
Histoplasma capsulatum, a facultative intracellular parasite of macrophages, grows within mononuclear cells of the P388D1 and IC-21 cell lines with a generation time comparable to that with which it grows in normal resident peritoneal macrophages (10 +/- 2 h). Recombinant murine gamma interferon (rMuIFN-gamma) activates P388D1 cells to express la antigens but not to inhibit the intracellular growth of H. capsulatum, alone or in combination with lipopolysaccharide. IC-21 cells also could not be activated to fungistasis with rMuIFN-gamma. Explanted resident peritoneal macrophages of the C57BL/6 (from which the IC-21 cell line derives), C3H/HeJ, DBA/2 (from which the P388D1 cell line derives), A/J, and SJL/J strains of mice were all stimulated by rMuIFN-gamma to inhibit the fungus.
Wu-Hsieh
B
B
Department of Microbiology and Immunology, University of California at Los Angeles, School of Medicine 90024.
Howard
D H
DH
D000818:Animals; D000935:Antifungal Agents / Q000276:immunology; D002460:Cell Line; D003593:Cytoplasm / Q000276:immunology / Q000382:microbiology*; D006658:Histoplasma / Q000254:growth & development* / Q000276:immunology; D007371:Interferon-gamma / Q000494:pharmacology*; D007941:Leukemia P388 / Q000276:immunology* / Q000382:microbiology; D007942:Leukemia, Experimental / Q000276:immunology*; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology* / Q000382:microbiology; D051379:Mice; D008805:Mice, Inbred A; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D011994:Recombinant Proteins
D000935:Antifungal Agents; D011994:Recombinant Proteins; D007371:Interferon-gamma