title: [New cloning vectors constructed in Bacillus].
abstract: Two new plasmids pNQ216 (4.1kp) and pNQ402 (2.8 kb) were constructed by combining the replication origin of the plasmid pNK289, a cryptic plasmid resides in B. pumilus 289, and the cat-86 gene from plasmid pPL601. These two plasmids can be maintained steadily in both B. subtilis and B. pumilus. The penetrance of these plasmids on LB medium with Cm (20 micrograms/ml) is 30% higher than that of pPL600. Thus both of these plasmids can be used as new cloning vectors in Bacillus.
authors.forename: M
authors.initials: M
authors.lastname: Qiao
authors.forename: B
authors.initials: B
authors.lastname: Cai
authors.forename: C
authors.initials: C
authors.lastname: Gao
authors.forename: Y
authors.initials: Y
authors.lastname: Lang
authors.forename: T
authors.initials: T
authors.lastname: Fan
authors.forename: T F
authors.initials: TF
authors.lastname: Su
authors.forename: R
authors.initials: R
authors.lastname: Jiang
mesh_terms: D001407:Bacillus / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors; D010957:Plasmids