title: Atypical transient state kinetics of recombinant human dihydrofolate reductase produced by hysteretic behavior. Comparison with dihydrofolate reductases from other sources.
abstract: The transient state kinetics of catalysis for dihydrofolate reductase (DHFR) from several enzyme sources including highly purified recombinant human enzyme (rHDHFR) have been examined. Like DHFR from Escherichia coli, the enzyme from Lactobacillus casei, and isoenzyme 2 from Streptococcus faecium exhibit a slow increase in activity upon addition of substrates to enzyme. No slow hysteresis of this type was detected with recombinant human DHFR (rHDHFR) or DHFR from chicken or bovine liver or L1210 mouse leukemia cells (MDHFR). In contrast, both rHDHFR and MDHFR exhibited a very rapid decrease in activity (t1/2 = 30 and 20 ms, respectively) during a phase that occurred after the first turnover of the enzyme but before establishment of the steady state. This intermediate phase was not observed for the bacterial enzymes or the avian enzyme, nor was it observed with a mutant of rHDHFR in which Phe-31 has been replaced by leucine. For rHDHFR the intermediate phase is not a consequence of product inhibition, substrate depletion, or enzyme instability. It may therefore be concluded that this unusual transient state kinetic behavior results from the existence of two conformers of the enzyme, one of which has a higher turnover number than the other with the equilibrium shifting in favor of the less active conformer during the course of catalysis. The equilibrium is particularly favorable for the less active conformer when NADP is present in the active site of rHDHFR, whereas bound tetrahydrofolate favors the more active conformer. The more active conformer has a 6-fold higher Km for dihydrofolate than does the less active conformer. The existence of these conformers is likely to produce cooperative behavior by rHDHFR in vivo.
authors.affiliation: Department of Biochemical and Clinical Pharmacology, St. Jude Children's Research Hospital, Memphis, Tennessee 38101.
authors.forename: J R
authors.initials: JR
authors.lastname: Appleman
authors.forename: W A
authors.initials: WA
authors.lastname: Beard
authors.forename: T J
authors.initials: TJ
authors.lastname: Delcamp
authors.forename: N J
authors.initials: NJ
authors.lastname: Prendergast
authors.forename: J H
authors.initials: JH
authors.lastname: Freisheim
authors.forename: R L
authors.initials: RL
authors.lastname: Blakley
mesh_terms: D000818:Animals; D002645:Chickens; D004926:Escherichia coli / Q000201:enzymology; D006801:Humans; D007700:Kinetics; D007780:Lacticaseibacillus casei / Q000201:enzymology; D007939:Leukemia L1210 / Q000201:enzymology; D008024:Ligands; D008099:Liver / Q000201:enzymology; D008433:Mathematics; D051379:Mice; D011994:Recombinant Proteins / Q000378:metabolism*; D013045:Species Specificity; D013291:Streptococcus / Q000201:enzymology; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics / Q000378:metabolism*
chemical_list: D008024:Ligands; D011994:Recombinant Proteins; D013762:Tetrahydrofolate Dehydrogenase