Induction of assembly of MHC class I heavy chains with beta 2microglobulin by interferon-gamma.
Assembly of histocompatibility class I heavy chains with beta 2microglobulin (beta 2m) is known to be necessary for cell surface expression. Studies on the H-2 class I deficient but interferon-gamma (IFN-gamma) inducible fibrosarcoma BC2 and the lung carcinoma CMT 64.5 showed that after transfection with allogeneic H-2 class I genes the class I proteins are expressed, but only intracellularly and not on the cell surface. In spite of the presence of beta 2m in the cells no association of the transfected class I chain with beta 2m was observed. However, stimulation with IFN-gamma induced assembly and subsequent surface expression. These findings show that the assembly of class I heavy chains with beta 2m is not a spontaneous event but appears to be regulated by cellular mechanisms the nature of which is still unknown.
Klar
D
D
Institute of Immunology and Genetics, German Cancer Research Center, Heidelberg.
Hämmerling
G J
GJ
D000818:Animals; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D005805:Genes, MHC Class I / Q000187:drug effects; D006183:H-2 Antigens / Q000235:genetics; D007371:Interferon-gamma / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D001613:beta 2-Microglobulin / Q000235:genetics / Q000378:metabolism*
D006183:H-2 Antigens; D001613:beta 2-Microglobulin; D007371:Interferon-gamma