title: Effects of dexamethasone on glucose transport by skeletal muscles of obese (ob/ob) mice.
abstract: Adrenalectomy normalized basal and insulin-stimulated glucose transport in the perfused hindquarter of the obese (ob/ob) mouse. Dexamethasone treatment of adrenalectomized obese mice for 9 days significantly reduced both the basal and insulin-stimulated uptake of (3H)-2-deoxy-D-glucose in hindlimbs of adrenalectomized mice to levels similar to those of intact ob/ob mice. Five hours after injection of dexamethasone in vivo and following 5 hours of perfusion in vitro there was a 10 percent reduction in basal 2-deoxy-D-glucose uptake in both groups. In muscles perfused with dexamethasone in vitro, the stimulation of 2-deoxy-D-glucose uptake by insulin was greater than in those muscles perfused with vehicle alone. Five hours of treatment in vivo also resulted in a significant increase in liver tyrosine aminotransferase activity, and a decrease in muscle glycogen synthetase activity ratio. These data suggest that the effect of glucocorticoids on lowering glucose transport in the skeletal muscle of obese ob/ob mice is a direct one.
authors.affiliation: Section of Diabetes and Clinical Nutrition, School of Medicine, University of Southern California, Los Angeles 90033.
authors.forename: K
authors.initials: K
authors.lastname: Ohshima
authors.forename: N S
authors.initials: NS
authors.lastname: Shargill
authors.forename: T M
authors.initials: TM
authors.lastname: Chan
authors.forename: G A
authors.initials: GA
authors.lastname: Bray
mesh_terms: D000315:Adrenalectomy; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D003907:Dexamethasone / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism*; D006614:Hindlimb; D008297:Male; D051379:Mice; D008820:Mice, Obese; D009132:Muscles / Q000187:drug effects* / Q000378:metabolism; D009765:Obesity / Q000378:metabolism*
chemical_list: D003907:Dexamethasone; D005947:Glucose