The use of high-performance liquid chromatography in the quality control of oxytocin, vasopressin and synthetic analogues.
Optimized C18 reversed-phase systems for oxytocin, desamino-oxytocin, lysine-vasopressin, ornithine-vasopressin and felypressin with gradient elution are discussed, focussing on precision, selectivity and ruggedness of the methods. Data from collaborative studies are presented, demonstrating the equivalence of high-performance liquid chromatography (HPLC) assays to bioassays. The findings suggest that HPLC is an excellent alternative to the time-consuming and less reliable animal testing.
Maxl
F
F
Quality Assurance of the Pharma Division, Sandoz Ltd, Basel, Switzerland.
Siehr
W
W
D000595:Amino Acid Sequence; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D005260:Female; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D010121:Oxytocin / Q000031:analogs & derivatives* / Q000032:analysis* / Q000494:pharmacology; D011786:Quality Control; D051381:Rats; D014590:Uterine Contraction / Q000187:drug effects; D014667:Vasopressins / Q000032:analysis* / Q000494:pharmacology
D014667:Vasopressins; D010121:Oxytocin