title: [Sensitivity of the culture of circulating leukocytes in the detection of Leishmania in the peripheral blood of patients with tegumentary leishmaniasis].
abstract: The possible presence of Leishmania in the peripheral blood of 60 patients with American cutaneous leishmaniasis was investigated by the culture of circulating leucocytes. Patients were selected with a variety of clinical forms of the disease and in different evolutionary stages of infection. Biopsies of skin and/or mucosal lesions were made in order to isolate the parasites, which were identified using monoclonal antibodies. 40 isolations were obtained, including 5 of Leishmania (Viannia) braziliensis, 3 L. (V.) guyanesis, 1 L. (V.) lainsoni, 13 L. (Leishmania) amazonensis and 18 which could only be identified as parasites of the braziliensis complex. Cultures of circulanting leucocytes were consistently negative, and the authors conclude that this method is of little use in diagnosis of cutaneous or mucocutaneous leishmaniasis.
authors.forename: F T
authors.initials: FT
authors.lastname: Silveira
authors.forename: R
authors.initials: R
authors.lastname: Lainson
authors.forename: J J
authors.initials: JJ
authors.lastname: Shaw
authors.forename: E A
authors.initials: EA
authors.lastname: Ishikawa
authors.forename: A A
authors.initials: AA
authors.lastname: Souza
authors.forename: R R
authors.initials: RR
authors.lastname: Braga
mesh_terms: D000293:Adolescent; D000818:Animals; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D006801:Humans; D007223:Infant; D007891:Leishmania / Q000302:isolation & purification*; D007896:Leishmaniasis / Q000097:blood / Q000175:diagnosis*; D007962:Leukocytes
vernacular_title: Sobre a sensibilidade da cultura de leucócitos circulantes na detecção de Leishmania no sangue periférico de pacientes com leishmaniose tegumentar.