Immunological separation of Entomophthorales genera.
Exoantigens from Erynia neoaphidis, Conidiobolus major, C. thromboides, C. obscurus, Zoophthora radicans, and Basidiobolus ranarum were obtained from culture filtrates of fungal material grown in a yeast extract, peptone dialysate, dextrose medium and were tested against specific hyperimmune antisera prepared from E. neoaphidis, C. major, C. thromboides, and B. ranarum by the immunodiffusion technique. Specific precipitins were observed for E. neoaphidis and B. ranarum, while cross-reactions were detected among C. major, C. thromboides, and C. obscurus. The results suggest that genera of Entomophthorales can be easily separated by this simple immunological procedure.
Toriello
C
C
Zerón
E
E
Latgé
J P
JP
Mier
T
T
D000946:Antigens, Fungal / Q000032:analysis / Q000276:immunology; D003429:Cross Reactions; D004773:Entomophthora / Q000145:classification* / Q000276:immunology; D005658:Fungi / Q000145:classification*; D007106:Immune Sera / Q000276:immunology; D005779:Immunodiffusion
D000946:Antigens, Fungal; D007106:Immune Sera