[A significance of dual parameter flow cytometric DNA analysis using the anti-keratin antibody].
Flow cytometric DNA analysis using the anti-cytokeratin antibody was carried out in order to estimate more reliable measurement in single cell suspension obtained from solid tumors. It was difficult to detect a DNA aneuploidy with DI of 2.0 by one parameter analysis of DNA. Whereas it could be detected easily by using dual parameter analysis of cytokeratin and DNA. And also, the pattern of DNA multiploidy could be selected for cytokeratin positive cell population by gate analysis.
Ishikawa
H
H
First Department of Surgery, Nagasaki University School of Medicine, Japan.
Tagawa
Y
Y
Miyashita
K
K
Yokota
M
M
Yasutake
T
T
Okada
D
D
Kunisaki
T
T
Tomita
M
M
D000906:Antibodies; D004273:DNA, Neoplasm / Q000032:analysis* / Q000235:genetics; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007633:Keratins / Q000276:immunology*; D008175:Lung Neoplasms / Q000235:genetics; D011003:Ploidies; D012004:Rectal Neoplasms / Q000235:genetics; D014407:Tumor Cells, Cultured
D000906:Antibodies; D004273:DNA, Neoplasm; D007633:Keratins