Rapid method for the detection of DNase of campylobacters.
A rapid agar diffusion method for the detection of DNase production of Campylobacter jejuni, C. coli, and C. pylori was developed. A strong pink zone indicating DNA hydrolysis was seen around the wells after 20 to 24 h of aerobic incubation at 37 degrees C. Pretreatment of cells with polymyxin B, which releases the cell-associated DNase, both shortened the time needed to read positive results to 8 h and increased the zone size.
Hänninen
M L
ML
Department of Food and Environmental Hygiene, College of Veterinary Medicine, Finland.
D002167:Campylobacter / Q000187:drug effects / Q000201:enzymology*; D002168:Campylobacter fetus / Q000187:drug effects / Q000201:enzymology*; D003851:Deoxyribonucleases / Q000032:analysis* / Q000096:biosynthesis; D005779:Immunodiffusion; D011112:Polymyxin B / Q000494:pharmacology
D003851:Deoxyribonucleases; D011112:Polymyxin B