The Drosophila Go alpha-like G protein gene produces multiple transcripts and is expressed in the nervous system and in ovaries.
Drosophila melanogaster cDNA clones coding for the alpha subunit of a Go-like G protein have been isolated. The sequence of two cDNA clones shows there is alternative splicing in the 5'-coding region which, on conceptual translation, would give rise to two proteins with slightly different amino termini. A partial genomic clone indicates there are four introns in the carboxyl-terminal half of the clone. Two transcripts, 3.8 and 5.3 kilobases long, are expressed at a high level in the heads of adult flies and also in larvae, pupae, and embryos. Hybridization of the cDNA probes to sections of adult flies indicates the RNA is present predominantly in nervous tissue (in the cortex of the brain and the thoracic ganglion); it is also expressed in the ovaries. Transcripts corresponding to both cDNAs are present in the central nervous system, but only one of them is found in detectable levels in the ovaries. The gene maps to 47A on the Drosophila second chromosome.
de Sousa
S M
SM
Department of Biochemistry, Howard Hughes Medical Institute, University of Washington, Seattle 98195.
Hoveland
L L
LL
Yarfitz
S
S
Hurley
J B
JB
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D015342:DNA Probes; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics*; D005260:Female; D019204:GTP-Binding Proteins / Q000235:genetics*; D015870:Gene Expression; D007438:Introns; D007814:Larva / Q000378:metabolism; D008969:Molecular Sequence Data; D009420:Nervous System / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D010053:Ovary / Q000378:metabolism*; D011679:Pupa / Q000378:metabolism; D012326:RNA Splicing; D015183:Restriction Mapping; D014158:Transcription, Genetic / Q000235:genetics*
D015342:DNA Probes; D004247:DNA; D019204:GTP-Binding Proteins