Transformed cells overexpress a cytosolic nucleic acid binding protein.
A protein blotting technique was used to identify a 57,000 dalton cytosolic nucleic-acid-binding protein found in neoplastically transformed cell lines. Specifically, greater amounts of this protein were found in Kirsten Murine Sarcoma Virus-, Simian Virus 40-, and methylcholanthrene-transformed Balb 3T3 cells than in comparable untransformed cells. An analogous protein was identified in other transformed mammalian cells. Increased levels of the DNA binding protein in sarcoma virus transformants were shown to be dependent on the continued maintenance of the transformed phenotype. The properties of this protein are compared to those of other previously reported nucleic acid binding proteins.
Stoler
D L
DL
Department of Molecular and Cellular Biology, Roswell Park Memorial Institute, Buffalo, New York 14263.
Manly
K F
KF
D000818:Animals; D001667:Binding, Competitive; D015153:Blotting, Western; D002352:Carrier Proteins / Q000378:metabolism*; D002451:Cell Compartmentation; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D007526:Isoelectric Point; D051379:Mice; D008970:Molecular Weight; D009841:Oligonucleotides / Q000378:metabolism; D011061:Poly A / Q000378:metabolism*; D011089:Polydeoxyribonucleotides / Q000378:metabolism; D011131:Polyribonucleotides / Q000378:metabolism; D016601:RNA-Binding Proteins
D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D009841:Oligonucleotides; D011089:Polydeoxyribonucleotides; D011131:Polyribonucleotides; D016601:RNA-Binding Proteins; D011061:Poly A