Liquid chromatography-thermospray mass spectrometry of DNA adducts formed with mitomycin C, porfiromycin and thiotepa.
High-performance liquid chromatography (HPLC) and thermospray mass spectrometry were combined for the analysis of DNA adducts formed from the interaction of the anticancer drugs mitomycin C, porfiromycin and thiotepa with calf thymus DNA. The adducts formed from reaction of mitomycin C and porfiromycin with DNA were separated from unmodified nucleosides by HPLC on a C18 column and identified by thermospray mass spectrometry. Thiotepa DNA adducts readily depurinated from DNA and were chromatographed and identified by thermospray liquid chromatography-mass spectrometry as the modified bases without the ribose moiety attached. The utility of thermospray mass spectrometry for the identification of microgram quantities of nucleoside adducts and depurinated base adducts of these anticancer drugs was demonstrated.
Musser
S M
SM
Department of Medicinal Chemistry and Pharmacognosy, School of Pharmacy, University of Maryland, Baltimore 21201.
Pan
S S
SS
Callery
P S
PS
D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004247:DNA / Q000032:analysis*; D006868:Hydrolysis; D013058:Mass Spectrometry / Q000379:methods; D016685:Mitomycin; D008937:Mitomycins / Q000032:analysis*; D011160:Porfiromycin / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D013852:Thiotepa / Q000032:analysis*; D013950:Thymus Gland / Q000378:metabolism
D008937:Mitomycins; D016685:Mitomycin; D004247:DNA; D013852:Thiotepa; D011160:Porfiromycin