title: Coated fibronectin induces adhesion in the human monocytic cell line U937.
abstract: The differentiation inducing effect of coated human fibronectin (HFN) on U937 was investigated. U937 cells usually proliferate without stimuli. HFN induced the adhesion of about 20% of U937 cells in a serum free medium. HFN did not inhibit cell growth nor induce CD14 expression. The adhesion inducing activity of HFN was completely blocked by addition of anti-HFN antibody. Phorbor 12-myristate 13-acetate (PMA), another differentiation inducing agent, inhibited the growth of U937 cells but did not induce adhesion in the serum free medium. These data imply that HFN has a differentiation inducing effect on a human monocytic cell line and could be used as a biological response modifier.
authors.affiliation: First Department of Internal Medicine, National Defense Medical College, Saitama, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: M
authors.initials: M
authors.lastname: Hara
authors.forename: K
authors.initials: K
authors.lastname: Norioka
authors.forename: A
authors.initials: A
authors.lastname: Kitani
authors.forename: M
authors.initials: M
authors.lastname: Harigai
authors.forename: W
authors.initials: W
authors.lastname: Hirose
authors.forename: T
authors.initials: T
authors.lastname: Hirose
authors.forename: M
authors.initials: M
authors.lastname: Kawakami
authors.forename: M
authors.initials: M
authors.lastname: Kawagoe
authors.forename: H
authors.initials: H
authors.lastname: Nakamura
mesh_terms: D015214:Antigens, Differentiation, Myelomonocytic; D002448:Cell Adhesion / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D005353:Fibronectins / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D018950:Lipopolysaccharide Receptors; D009000:Monocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology
chemical_list: D015214:Antigens, Differentiation, Myelomonocytic; D005353:Fibronectins; D018950:Lipopolysaccharide Receptors