Demonstration of the increase in serine esterase-positive T cells in hairy-cell leukemia patients undergoing alpha-interferon therapy.
An increased number of serine esterase non-B lymphocytes including CD4+ helper/cytotoxic cells were observed in hairy-cell leukemia patients during (56 +/- 18) and immediately post (52 +/- 16) alpha-interferon therapy compared with pretreatment status (17 +/- 5) and normal donors (29 +/- 3). This increase in lymphocytes bearing one of the putative cytotoxicity-linked proteins was paralleled by a higher cytotoxicity towards K562 cells in the NK and LDCC assays during therapy and similarly, but to a much reduced level, against the hairy-cell derived JOK-1 target cells. Phenotypically, a higher percentage of non-B cells also expressing DR or CD11 antigens were seen in the HCL patients during IFN therapy. In lymphocyte target binding assays, a high affinity of effector to target conjugate formation was observed when either of the hairy cell lines JOK-1 or Hair-M was chosen as targets for IFN-therapy effector cells. Although predominantly CD4+ cells exhibited the in vitro affinity for effector/target conjugation with autologous HCs, the polarisation of serine esterase activity towards the E/T membrane contact area was shown by both CD4+ and CD8+ cells during a short incubation period and before target cell death.
Wagner
L
L
Department of Haematology, University College Hospital, London, U.K.
Goldstone
A H
AH
Worman
C P
CP
D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D016827:CD8 Antigens; D003602:Cytotoxicity, Immunologic; D004950:Esterases / Q000097:blood*; D006801:Humans; D007370:Interferon Type I / Q000627:therapeutic use*; D007694:Killer Cells, Natural / Q000276:immunology; D007943:Leukemia, Hairy Cell / Q000201:enzymology / Q000276:immunology* / Q000628:therapy; D013601:T-Lymphocytes / Q000201:enzymology* / Q000276:immunology
D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D016827:CD8 Antigens; D007370:Interferon Type I; D004950:Esterases; C022635:serine esterase