Immunological and chemical characterization of hamster brain polyribosomes-cytomatrix complexes.
We have studied the cytoskeletal nature of a brain subcellular fraction previously shown to contain polyribosomes. We have identified the major proteins of this fraction by electrophoretic comparison to a standard cytoskeletal fraction and by immunodetection. These methods have shown the presence of actin, glial fibrillary acidic protein, and neurofilament triplet proteins. We have also studied the effect of various ions and nonionic detergents on the stability of this structure. It was stable in presence of Triton X-100 up to 2% but disrupted by 200 mM K+ acetate.
Bouhtiauy
I
I
Départment de chimie et biochimie, Université de Moncton, Canada.
Choukri
Y
Y
Turpin
C
C
Gauthier
D
D
D000199:Actins / Q000032:analysis; D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D001921:Brain / Q000648:ultrastructure*; D002458:Cell Fractionation; D006224:Cricetinae; D003598:Cytoskeletal Proteins / Q000032:analysis*; D003599:Cytoskeleton / Q000032:analysis / Q000648:ultrastructure; D003902:Detergents / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D007381:Intermediate Filament Proteins / Q000032:analysis; D008970:Molecular Weight; D016900:Neurofilament Proteins; D011132:Polyribosomes / Q000032:analysis* / Q000648:ultrastructure; D013347:Subcellular Fractions / Q000032:analysis*
D000199:Actins; D000911:Antibodies, Monoclonal; D003598:Cytoskeletal Proteins; D003902:Detergents; D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; D016900:Neurofilament Proteins