A novel second-generation polyolefin container for storage of single-donor apheresis platelets.
A non-blow-molded LE-2 polyolefin (PO) container was developed to store single-donor apheresis platelet concentrates (PCs) processed by the Haemonetics Plasma Collecting System (PCS) at 22 degrees C for over 1 day. We molded PO containers with heat-sealing of light-weight polymer alloy films with a thickness of 0.25 mm. The film was made of polymer blends consisting of polypropylene, styrene ethylene butylene styrene-block copolymer, and ethylene ethylacrylate copolymer. The PO container with enough strength and flexibility in routine practice has 2 and 1.6 times higher oxygen and carbon dioxide gas transfer properties than standard polyvinyl chloride (PVC) plastic containers. PCs (1-1.9 x 10(11) platelets) processed from 450 ml platelet-rich plasma were stored in 0.6-liter PO containers at 22 degrees C with flatbed agitation for up to 6 days. The pH of PCs was well maintained at the mean values of 7.0 in PO containers after 6 days in the well-oxygenated condition. The energy metabolism of stored platelets was determined. Oxygen consumption rates of platelets stored in PO containers averaged 1.5 nmol/min/10(9) platelets. The rates of glucose consumption and lactate production were 0.4 and 0.8 nmol/min/10(11) platelets, respectively. The rates of adenosine triphosphate (ATP) generation of platelets, 9.7 nmol/min/10(9) platelets, in PO containers did not differ from those in the PVC containers. Aggregation responses to adenosine diphosphate and hypotonic shock response of platelets were better maintained in PO containers. The morphological changes into sphere forms with projections and the appearance of unclassified forms were more frequently observed in PO than in PVC containers.(ABSTRACT TRUNCATED AT 250 WORDS)
Shimizu
T
T
Aichi Center, Japanese Red Cross Blood Transfusion Service, Japan.
Kouketsu
K
K
Kamiya
T
T
Futagawa
H
H
Hirose
S
S
D001781:Blood Component Removal; D001792:Blood Platelets; D001793:Blood Preservation; D002245:Carbon Dioxide / Q000378:metabolism; D004058:Diffusion; D006801:Humans; D010100:Oxygen / Q000378:metabolism; D010539:Permeability; D010969:Plastics; D011090:Polyenes; D011143:Polyvinyl Chloride
D010969:Plastics; D011090:Polyenes; D002245:Carbon Dioxide; C035051:PL 732; D011143:Polyvinyl Chloride; D010100:Oxygen