title: AIDS-antiviral sulfolipids from cyanobacteria (blue-green algae).
abstract: A recently developed tetrazolium-based microculture assay was used to screen extracts of cultured cyanobacteria (blue-green algae) for inhibition of the cytopathic effects of the human immunodeficiency virus (HIV-1), which is implicated as a causative agent of AIDS. A number of extracts were found to be remarkably active against the AIDS virus. A new class of HIV-1-inhibitory compounds, the sulfonic acid-containing glycolipids, was discovered through the use of the microculture assay to guide the fractionation and purification process. The pure compounds were active against HIV-1 in cultured human lymphoblastoid CEM, MT-2, LDV-7, and C3-44 cell lines in the tetrazolium assay as well as in p24 viral protein and syncytium formation assays.
authors.affiliation: Division of Cancer Treatment, National Cancer Institute, Bethesda, MD.
authors.forename: K R
authors.initials: KR
authors.lastname: Gustafson
authors.forename: J H
authors.initials: JH
authors.lastname: Cardellina
authors.forename: R W
authors.initials: RW
authors.lastname: Fuller
authors.forename: O S
authors.initials: OS
authors.lastname: Weislow
authors.forename: R F
authors.initials: RF
authors.lastname: Kiser
authors.forename: K M
authors.initials: KM
authors.lastname: Snader
authors.forename: G M
authors.initials: GM
authors.lastname: Patterson
authors.forename: M R
authors.initials: MR
authors.lastname: Boyd
mesh_terms: D000998:Antiviral Agents / Q000302:isolation & purification; D055598:Chemical Phenomena; D002621:Chemistry; D000458:Cyanobacteria / Q000032:analysis*; D006678:HIV / Q000187:drug effects*; D016655:HIV Core Protein p24; D008055:Lipids / Q000302:isolation & purification / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000379:methods; D012191:Retroviridae Proteins / Q000032:analysis; D013778:Tetrazolium Salts
chemical_list: D000998:Antiviral Agents; D016655:HIV Core Protein p24; D008055:Lipids; D012191:Retroviridae Proteins; D013778:Tetrazolium Salts; C015518:sulfolipids