Immunocytochemistry and image cytometry of progesterone receptors in breast carcinoma imprints. Expression of tissular and cellular heterogeneity: relations to biochemical assay, clinical and histological parameters and DNA analysis.
Breast cancer imprints from 93 patients were assayed for the presence of progesterone receptors (PgR) using a monoclonal antibody (Transbio) and an immunocytochemical assay (ICA) method which stains only the epithelial cell nuclei. Results were compared with conventional biochemical PgR determinations (DCCA) and were in qualitative agreement in 86% of the cases. Quantitative analyses were done on PgR-ICA- and Feulgen-stained imprints from 32 tumours using a SAMBA 2005 cell image processor. Results obtained showed a high correlation between DCCA values and the P product derived from the mean PgR concentration of marked tumour cells and the percentage of marked cells. Intra-tumoral and intra-cell heterogeneity were featured and showed relation to tumour differentiation and size.
Seigneurin
D
D
Quantitative Cytology Group, University Joseph Fourier, Grenoble, France.
Cohen
O
O
Louis
J
J
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor / Q000032:analysis*; D001943:Breast Neoplasms / Q000737:chemistry / Q000473:pathology*; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000737:chemistry / Q000473:pathology*; D002467:Cell Nucleus / Q000737:chemistry; D004396:Coloring Agents; D003592:Cytophotometry / Q000295:instrumentation / Q000379:methods*; D004273:DNA, Neoplasm / Q000032:analysis; D004848:Epithelium / Q000737:chemistry; D005456:Fluorescent Dyes; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation; D008856:Microscopy, Fluorescence / Q000295:instrumentation; D009363:Neoplasm Proteins / Q000032:analysis; D011980:Receptors, Progesterone / Q000032:analysis* / Q000276:immunology; D012394:Rosaniline Dyes; D013194:Staining and Labeling; D013690:Television
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D004396:Coloring Agents; D004273:DNA, Neoplasm; C007722:Feulgen stain; D005456:Fluorescent Dyes; D009363:Neoplasm Proteins; D011980:Receptors, Progesterone; D012394:Rosaniline Dyes