Immunologic, histopathologic, and bacteriologic responses of five strains of mice to Brucella abortus strain 2308.
A study was conducted to establish baseline data on Brucella abortus infection induced in 5 strains of mice (CBA/NJ, BALB/c, CD-1, C3H/HeN, and C3H/HeJ). The strains were compared on the basis of immunologic, histopathologic, and bacteriologic responses. There were 4 treatment groups for each strain of mice: (1) vaccinated with homologous lipopolysaccharide and challenge exposed to B abortus strain 2308; (2) not vaccinated but challenge exposed; (3) vaccinated and not challenge exposed; and (4) not vaccinated and not challenge exposed. Results indicated that mice can be used for comparative studies on the pathogenesis and immunogenesis of B abortus infections; strains of mice may vary in their responses to Brucella infection, regardless of their vaccination status. Bacteriologic and immunologic responses in mouse strains BALB/c, CD-1, C3H/HeN, and C3H/HeJ, but not those of CBA/NJ, were extrapolative among strains.
Pugh
G W
GW
USDA, Agricultural Research Service, National Animal Disease Center, Ames, IA 50010.
Zehr
E S
ES
Meador
V P
VP
Phillips
M
M
McDonald
T J
TJ
Deyoe
B L
BL
D000818:Animals; D002003:Brucella abortus / Q000276:immunology*; D002006:Brucellosis / Q000276:immunology* / Q000517:prevention & control; D015169:Colony Count, Microbial; D004797:Enzyme-Linked Immunosorbent Assay; D005189:False Positive Reactions; D005779:Immunodiffusion; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008808:Mice, Inbred CBA; D009929:Organ Size; D011237:Predictive Value of Tests; D013154:Spleen / Q000382:microbiology / Q000473:pathology
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008070:Lipopolysaccharides