Nucleoside 5'-triphosphates modified at sugar residues as substrates for DNA polymerase from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius.
The ability of a wide variety of nucleoside 5'-triphosphates with modified sugar moiety to serve as substrates in DNA synthesis catalyzed by DNA polymerase A from the archaebacterium Sulfolobus acidocaldarius was studied. Most of the dNTP analogs tested are shown to be specific terminating substrates for the synthesis irreversibly blocking further elongation of a nascent chain. The most powerful inhibitors were found to be 3'-amino derivatives of deoxy and arabino nucleoside triphosphates, while specific reverse transcriptase inhibitors, 3'-azido and 3'-methoxy derivatives of dNTP, were found to be inactive.
Chinchaladze
D Z
DZ
Institute of Molecular Biology and Biological Physics, Georgian SSR Academy of Sciences, Tbilisi, U.S.S.R.
Prangishvili
D A
DA
Scamrov
A V
AV
Beabealashvili
R S
RS
Dyatkina
N B
NB
Krayevsky
A A
AA
D001105:Archaea / Q000201:enzymology*; D001419:Bacteria / Q000201:enzymology*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000096:biosynthesis; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D003854:Deoxyribonucleotides / Q000378:metabolism*; D008969:Molecular Sequence Data; D013379:Substrate Specificity
D004269:DNA, Bacterial; D003854:Deoxyribonucleotides; D004259:DNA-Directed DNA Polymerase