Ultrastructural localization of Cu, Zn-SOD in hepatocytes of patients with various liver diseases.
The ultrastructural localization of copper, zinc-superoxide dismutase (Cu, Zn-SOD) in the liver of patients with acute hepatitis, chronic hepatitis, liver cirrhosis and alcoholic fatty liver was studied by means of the indirect immunoperoxidase technique. In hepatocytes Cu, Zn-SOD was found to be localized in perinuclear cisternae, rough endoplasmic reticulum (rER), vesicles and Golgi apparatus. The Cu, Zn-SOD was also detected around the lipid droplets in hepatocytes as well as on the cytoplasmic membrane in cases of liver cirrhosis. These findings suggest that Cu, Zn-SOD is produced in the rER in hepatocytes and protects the cells from cellular injury caused by superoxide anion radical in various disorders of the liver.
Saito
T
T
Second Department of Internal Medicine, Yamagata University, School of Medicine, Japan.
Shinzawa
H
H
Togashi
H
H
Wakabayashi
H
H
Ukai
K
K
Takahashi
T
T
Ishikawa
M
M
Dobashi
M
M
Imai
Y
Y
D004721:Endoplasmic Reticulum / Q000201:enzymology; D006505:Hepatitis / Q000201:enzymology / Q000473:pathology; D006651:Histocytochemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D008099:Liver / Q000201:enzymology / Q000473:pathology* / Q000648:ultrastructure; D008103:Liver Cirrhosis / Q000201:enzymology / Q000473:pathology; D008107:Liver Diseases / Q000201:enzymology / Q000473:pathology*; D008854:Microscopy, Electron; D016253:Microscopy, Immunoelectron; D013194:Staining and Labeling; D013482:Superoxide Dismutase / Q000378:metabolism*
D013482:Superoxide Dismutase