title: Characterization of the Salmonella paratyphi C Vi polysaccharide.
abstract: The Vi capsular polysaccharide (Vi) is both a virulence factor and a protective antigen of Salmonella typhi; its pathogenic role for Salmonella paratyphi C is less well understood. We found no differences between the antigenic and immunogenic properties and the structure of the Vi from representative strains of S. paratyphi C, S. typhi, and Citrobacter freundii. There were, however, differences in both the amount produced per cell and the degree of association with the cell among the Vi from the three species of Enterobacteriaceae. S. paratyphi C produced less Vi than both the wild-type S. typhi and C. freundii did, and it showed the fastest release of Vi into the media. These findings may provide an explanation for the inability of the Vi to inhibit completely the agglutination of S. paratyphi C by anti-O sera. In an outbreak of enteric fever caused by S. paratyphi C, 66 of 78 isolates (85%) were Vi positive.
authors.affiliation: Laboratory of Developmental and Molecular Immunity, National Institute of Child Health and Human Development, Bethesda, Maryland 20892.
authors.forename: E M
authors.initials: EM
authors.lastname: Daniels
authors.forename: R
authors.initials: R
authors.lastname: Schneerson
authors.forename: W M
authors.initials: WM
authors.lastname: Egan
authors.forename: S C
authors.initials: SC
authors.lastname: Szu
authors.forename: J B
authors.initials: JB
authors.lastname: Robbins
mesh_terms: D000293:Adolescent; D000328:Adult; D000362:Agar; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D002954:Citrobacter / Q000378:metabolism; D006801:Humans; D007106:Immune Sera; D005779:Immunodiffusion; D007223:Infant; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010284:Paratyphoid Fever / Q000382:microbiology; D011135:Polysaccharides, Bacterial / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology; D011817:Rabbits; D012475:Salmonella / Q000276:immunology*; D012479:Salmonella paratyphi C / Q000254:growth & development / Q000276:immunology* / Q000378:metabolism; D012485:Salmonella typhi / Q000378:metabolism
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007106:Immune Sera; D011135:Polysaccharides, Bacterial; D000362:Agar