Studies on the chemical constitution of cell wall lipo-oligosaccharide from Campylobacter coli Labet 227.
The lipo-oligosaccharide (LOS) from Campylobacter coli Labet 227 was extracted by aqueous phenol. After delipidation and gel chromatography, two oligosaccharides were isolated. The higher molecular weight material OS (I) which was estimated to contain six to seven sugar units was found to contain glucose, galactose, 2-acetamido-2-deoxyglucose, 2-acetamido-2-deoxygalactose and heptose. Analysis of the partially methylated alditol acetates by g.c.-m.s. revealed the presence of terminal hexoses, a 1.3-linked hexose, a terminal heptose, a 1,2,3-linked heptose as well as smaller quantities of a 1,3,4-linked heptose. 1H-n.m.r. spectra showed signals corresponding to six anomeric protons. The signals which corresponded to the methyl protons of the acetamido side chain confirmed that the acetamido forms of both amino sugars were present in OS (I). The lower molecular weight material OS (II) which was estimated to contain four sugar units was found to contain glucose, 2-acetamido-2-deoxy-galactose and very small quantities of heptose. It thus appears that OS (I) and OS (II) are the core oligosaccharides elaborated by this micro-organism. The possibility of a heterogeneous core is thus presented. The fatty acids present in the LOS were mainly 3-hydroxytetradecanoic acid, n-hexadecanoic acid and trace amounts of n-tetradecanoic acid and n-octadecanoic acid.
Adeyeye
A
A
Chemistry Department, University of Lagos, Yaba, Nigeria.
Ogunlesi
M
M
Odugbemi
T
T
D002167:Campylobacter / Q000032:analysis*; D002473:Cell Wall / Q000737:chemistry*; D002850:Chromatography, Gel; D005227:Fatty Acids / Q000032:analysis; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006539:Heptoses / Q000032:analysis; D008070:Lipopolysaccharides / Q000032:analysis / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008745:Methylation; D008970:Molecular Weight
D005227:Fatty Acids; D006539:Heptoses; D008070:Lipopolysaccharides; C023023:lipid-linked oligosaccharides; D005947:Glucose; D005690:Galactose