Inhibition of mouse bone marrow granulocyte-macrophage colony formation by factors derived from natural killer cells: an in vitro model for hybrid resistance.
Investigations were performed to study whether soluble factors produced by NK-cells could mediate "hybrid resistance" in vitro. NK-cells enriched from spleens of B6D2F1 hybrid mice were incubated with parental B6 bone marrow, and the effect of the derived supernatants on the development of granulocyte-macrophage colony forming cells (GM-CFC) was assessed. Cell free supernatants obtained from low density cells (LDC) of B6D2F1 hybrids stimulated with bone marrow cells (BMC) from B6 mice inhibited GM-CFC formation. The inhibition was similar using B6, D2 or B6D2F1 bone marrow cells as the targets for GM-CFC growth. Our findings suggest that NK cells from F1 hybrid mice when stimulated with BMC from B6 mice release inhibitory factors, different from IFN-gamma and that this production may represent a mechanism of natural resistance to parental H-2b bone marrow grafts.
Lukomska
B
B
Department for Surgical Research & Transplantology, Medical Research Center, Polish Academy of Sciences, Warsaw.
Hansson
M
M
Kiessling
R
R
D000818:Animals; D000911:Antibodies, Monoclonal; D001853:Bone Marrow / Q000276:immunology*; D002499:Centrifugation, Density Gradient; D003114:Colony-Forming Units Assay; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006098:Granulocytes / Q000276:immunology*; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D007371:Interferon-gamma / Q000032:analysis; D007694:Killer Cells, Natural / Q000276:immunology*; D055646:Killer Factors, Yeast; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D011506:Proteins / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology
D000911:Antibodies, Monoclonal; D055646:Killer Factors, Yeast; D011506:Proteins; D007371:Interferon-gamma