Effect of phosphatidylethanolamine and its constituent base on the metabolism of linoleic acid in rat liver.
Dietary phosphatidylethanolamine (PE) contributes the circulatory and hepatic free-ethanolamine in rats (Ikeda et al. (1987) Biochim. Biophys. Acta 921, 245). A role for circulatory ethanolamine has not been defined; however, our recent studies have shown that exogenous ethanolamine influences cholesterol and linoleic acid metabolism in rats (Imaizumi et al. (1983) J. Nutr. 113, 2403). In order to understand the role of dietary PE the effects of PE and its base on the hepatic metabolism of linoleic acid were investigated in vivo and in primary cultured hepatocytes in rats. Dietary PE increased the plasmic level of ethanolamine from 37 to 52 microM and decreased the ratio of arachidonate to linoleate in hepatic phospholipids. Activity of hepatic delta 6-desaturase decreased in rats given PE and the desaturation of [14C]linoleate in the cultured hepatocytes decreased by the addition of ethanolamine. Secretion [14C]linoleate labeled very-low-density lipoprotein from the cultured hepatocytes decreased by the addition of ethanolamine. Dietary PE caused an increased formation of CO2 from [14C]acetate by liver slices, and ethanolamine added to the hepatocytes caused an increased oxidation of [14C]linoleate and a suppression of fatty acid synthesis from [3H]serine. These results suggest that ethanolamine derived from the dietary PE plays a regulatory role in the linoleate metabolism in the liver.
Imaizumi
K
K
Department of Food Science and Technology, School of Agriculture, Kyushu University, Fukuoka, Japan.
Sakono
M
M
Mawatari
K
K
Murata
M
M
Sugano
M
M
D000818:Animals; D002478:Cells, Cultured; D004041:Dietary Fats / Q000494:pharmacology*; D019856:Ethanolamine; D004983:Ethanolamines / Q000494:pharmacology*; D044943:Fatty Acid Desaturases / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism*; D050602:Linoleoyl-CoA Desaturase; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D010713:Phosphatidylcholines / Q000096:biosynthesis*; D010714:Phosphatidylethanolamines / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D012694:Serine / Q000378:metabolism
D004041:Dietary Fats; D004983:Ethanolamines; D008041:Linoleic Acids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D012694:Serine; D019856:Ethanolamine; D019787:Linoleic Acid; D044943:Fatty Acid Desaturases; D050602:Linoleoyl-CoA Desaturase; D005990:Glycerol