title: Detection of Rickettsia rickettsii DNA in clinical specimens by using polymerase chain reaction technology.
abstract: A polymerase chain reaction (PCR) procedure for detecting rickettsial DNA was developed and shown to be specific for Rickettsia rickettsii and R. conorii, the etiologic agents of Rocky Mountain spotted fever (RMSF) and Boutonneuse fever, respectively. Blood clots were obtained from nine confirmed RMSF patients and six controls and analyzed for the presence of rickettsial DNA by the PCR method. A defined region of the rickettsial genome was successfully amplified from seven of the nine clinical specimens tested; all six control specimens gave negative results. These findings indicate that R. rickettsii can be detected early after the onset of RMSF, possibly facilitating the decision regarding appropriate antibiotic therapy for some patients. Further refinement of PCR technology could make this procedure a mainstay in the clinical laboratory.
authors.affiliation: Division of Viral and Rickettsial Diseases, Centers for Disease Control, Atlanta, Georgia 30333.
authors.forename: T
authors.initials: T
authors.lastname: Tzianabos
authors.forename: B E
authors.initials: BE
authors.lastname: Anderson
authors.forename: J E
authors.initials: JE
authors.lastname: McDade
mesh_terms: D001907:Boutonneuse Fever / Q000175:diagnosis; D004269:DNA, Bacterial / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D005784:Gene Amplification; D006801:Humans; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D012281:Rickettsia / Q000235:genetics / Q000302:isolation & purification; D012284:Rickettsia rickettsii / Q000235:genetics / Q000302:isolation & purification*; D012373:Rocky Mountain Spotted Fever / Q000175:diagnosis*
chemical_list: D004269:DNA, Bacterial; D015345:Oligonucleotide Probes