Effects of solute conditions on the relative affinities of the oligonucleotides d(G-C)5 and d(A-T)5 for the anthracycline drug 2-fluoro-4-demethoxydaunomycin.
19F NMR has been used to show that changes in NaCl concentration, as well as the presence of lysine or arginine, affect the equilibrium distribution of the synthetic anthracycline 2-fluoro-4-demethoxydaunomycin (2FD) between binding sites on d(G-C)5 and d(A-T)5 in a 1:1:1 molar aqueous system: 2FD/d(G-C)5/d(A-T)5. Varying the pH between 6.2 and 7.7 had no effect. NaCl concentrations below 0.1 M led to a d(G-C)5 preference while above 0.1 M a preference for d(A-T)5 was observed. At comparable solute concentrations, use of either lysine or arginine resulted in a significant drug preference for d(G-C)5 compared to systems containing only NaCl.
Hammer
B C
BC
Department of Chemistry, University College, University of New South Wales, ACT, Australia.
Russell
R A
RA
Warrener
R N
RN
Collins
J G
JG
D001120:Arginine / Q000494:pharmacology; D001665:Binding Sites; D003630:Daunorubicin / Q000031:analogs & derivatives* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008239:Lysine / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D011067:Poly dA-dT / Q000378:metabolism*; D011089:Polydeoxyribonucleotides / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology
D011089:Polydeoxyribonucleotides; C058400:2-fluoro-4-demethoxydaunomycin; D011067:Poly dA-dT; C019530:poly(dC-dG); D012965:Sodium Chloride; D001120:Arginine; D008239:Lysine; D003630:Daunorubicin