Characterization of an amyloid beta precursor protein that binds heparin and contains tyrosine sulfate.
A secreted form of the amyloid beta protein precursor was isolated from the growth conditioned medium of the PC12 sympathetic nerve-like cell line. This protein is recognized by an antiserum that detects a protein of 140 kDa and a less abundant species of 115 kDa on NaDodSO4/acrylamide gels. The amyloid precursor proteins contain O-linked sugars and tyrosine sulfate and bind to the glycosaminoglycan heparin. These results suggest a role for extracellular sulfated glycoproteins in the pathogenesis of Alzheimer disease.
Schubert
D
D
Salk Institute for Biological Studies, San Diego, CA 92138.
LaCorbiere
M
M
Saitoh
T
T
Cole
G
G
D000310:Adrenal Gland Neoplasms / Q000378:metabolism; D000544:Alzheimer Disease; D000682:Amyloid / Q000032:analysis / Q000378:metabolism*; D016564:Amyloid beta-Protein Precursor; D000818:Animals; D015153:Blotting, Western; D002241:Carbohydrates / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006493:Heparin / Q000378:metabolism*; D007118:Immunoassay; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis / Q000378:metabolism; D010673:Pheochromocytoma / Q000378:metabolism; D011480:Protease Inhibitors; D011498:Protein Precursors / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D014407:Tumor Cells, Cultured; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000032:analysis
D000682:Amyloid; D016564:Amyloid beta-Protein Precursor; D002241:Carbohydrates; D010446:Peptide Fragments; D011480:Protease Inhibitors; D011498:Protein Precursors; C005662:tyrosine O-sulfate; D014443:Tyrosine; D006493:Heparin