title: Rapid method for identifying subclones bridging gaps between contiguous DNA sequences.
abstract: Randomly sequencing of a limited number of unidirectional deletion subclones efficiently generated contiguous DNA sequences with some gaps between them. Gap-bridging subclones were identified by agarose gel electrophoresis. Those located at either end of each individual contiguous sequence were used as standard marker molecules; the subclones between them proved to be correctly identified in most cases. A 4064 bp DNA fragment containing the psr gene was sequenced with the strategy described in this paper.
authors.affiliation: Shanghai Institute of Biochemistry, Academia sinica.
authors.forename: Y Y
authors.initials: YY
authors.lastname: Lu
authors.forename: F D
authors.initials: FD
authors.lastname: Ni
authors.forename: G F
authors.initials: GF
authors.lastname: Hong
mesh_terms: D001483:Base Sequence; D002872:Chromosome Deletion; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA; D004587:Electrophoresis, Agar Gel
chemical_list: D004247:DNA