An antigen expressed by murine CD8+ T cells and activated B cells.
A monoclonal antibody, H1F5, is described that reacts with a subset of Lyt-2 (CD8) mouse T cells and LPS-activated B cells. In both lymph nodes and spleen of BALB/c mice, the H1F5 antigen is coexpressed approximately on 20%-30% of the CD8+ T cells and approximately on 91% of LPS-activated B cells. In the thymus, few cells (less than 1%) are positive for the marker, but no correlation could be demonstrated with markers for mature T cells such as MEL-14 and PNA expression. Elimination of H1F5+ cells by complement lysis led to a 30%-50% reduction of specific lysis as measured in a primary allo CTL, indicating that the cytotoxic effector cells are injured. The relationship of this marker and other antigenic determinants on lymphocytes is discussed.
Breel
M
M
Department of Histology, Free University, Amsterdam, The Netherlands.
Griffioen
A
A
ter Hart
H
H
Kraal
G
G
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology*; D016827:CD8 Antigens; D002469:Cell Separation; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D008198:Lymph Nodes / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D016827:CD8 Antigens