title: The interaction between human blood-coagulation factor VIII and von Willebrand factor. Characterization of a high-affinity binding site on factor VIII.
abstract: The interaction between human Factor VIII and immobilized multimeric von Willebrand Factor (vWF) was characterized. Equilibrium binding studies indicated the presence of multiple classes of Factor VIII-binding sites on vWF. The high-affinity binding (Kd = 2.1 x 10(-10) M) was restricted to only 1-2% of the vWF subunits. Competition studies with monoclonal antibodies with known epitopes demonstrated that the Factor VIII sequence Lys1673-Arg1689 is involved in the high-affinity interaction with vWF.
authors.affiliation: Department of Blood Coagulation, Central Laboratory of The Netherlands Red Cross Blood Transfusion Service, Amsterdam.
authors.forename: A
authors.initials: A
authors.lastname: Leyte
authors.forename: M P
authors.initials: MP
authors.lastname: Verbeet
authors.forename: T
authors.initials: T
authors.lastname: Brodniewicz-Proba
authors.forename: J A
authors.initials: JA
authors.lastname: Van Mourik
authors.forename: K
authors.initials: K
authors.lastname: Mertens
mesh_terms: D000595:Amino Acid Sequence; D005169:Factor VIII / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D013816:Thermodynamics; D014841:von Willebrand Factor / Q000378:metabolism*
chemical_list: D014841:von Willebrand Factor; D005169:Factor VIII