title: Evaluation of trehalose-mannitol broth for differentiation of Staphylococcus epidermidis from other coagulase-negative staphylococcal species.
abstract: Trehalose-mannitol agar and various broth formulations thereof were evaluated for the differentiation of Staphylococcus epidermidis from other common coagulase-negative staphylococcal species isolated from human blood cultures. While this differentiation was accurately made on trehalose-mannitol agar in 18 h, modification of the medium to a broth formulation containing 2% each trehalose and mannitol in 0.2 ml of purple broth base provided greater than 95% accuracy when a heavy inoculum was incubated on a rotary shaker for at least 4 h.
authors.affiliation: Department of Microbiology, Cleveland Clinic Foundation, Ohio 44195-5140.
authors.forename: C C
authors.initials: CC
authors.lastname: Knapp
authors.forename: J A
authors.initials: JA
authors.lastname: Washington
mesh_terms: D003470:Culture Media; D004187:Disaccharides; D006801:Humans; D008353:Mannitol; D013203:Staphylococcal Infections / Q000175:diagnosis*; D013212:Staphylococcus epidermidis / Q000302:isolation & purification*; D014199:Trehalose
chemical_list: D003470:Culture Media; D004187:Disaccharides; D008353:Mannitol; D014199:Trehalose