The effect of immunomodulators on PHA or gamma-IFN induced release of neopterin from purified macrophages and peripheral blood mononuclear cells.
Immunomodulators cause changes in neopterin-release from purified macrophages or peripheral blood mononuclear cells by affecting the macrophage and T cell subsets activity, the intracellular cGMP/cAMP balance, or the intracellular pteridines-related biochemical pathways. Increased neopterin release was achieved by gamma-IFN or its inducers (PHA, IL-2), by interfering with the biopterin production by increased levels of cGMP or by decreasing the activity of the T suppressor cells. The released neopterin levels decreased due to decreased macrophage and T-helper cell activity or due to increased levels of cAMP. The in vitro effect of the immunomodulators has to be taken into account when assessing the neopterin levels in immunomodulators-treated patients.
Barak
M
M
Microbiology Department, Faculty of Medicine, Technion, Haifa, Israel.
Merzbach
D
D
Gruener
N
N
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D001708:Biopterins / Q000031:analogs & derivatives* / Q000378:metabolism; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology*; D008264:Macrophages / Q000378:metabolism*; D019798:Neopterin; D010835:Phytohemagglutinins / Q000494:pharmacology*; D011863:Radioimmunoassay; D013601:T-Lymphocytes / Q000378:metabolism
D000276:Adjuvants, Immunologic; D007166:Immunosuppressive Agents; D010835:Phytohemagglutinins; D001708:Biopterins; D019798:Neopterin; D007371:Interferon-gamma