title: A linkage map of the mouse immunoglobulin lambda light chain locus.
abstract: The mouse immunoglobulin lambda light chain locus has been linked using field inversion gel electrophoresis. The lambda light chain locus classically contains two V and four J-C gene segments in inbred mouse strains, and was physically mapped in the BALB/c cell line Wehi-3 which contains unrearranged lambda light chain gene segments. The locus is relatively small and spans 300 kb, as defined by a variety of single and double digests using methylation-sensitive restriction enzymes. The order of the lambda gene segments is V2-J2C2J4C4-V1-J3C3J1C1, as was originally proposed. No evidence for nonmethylated CpG rich areas (HTF islands) within the region was found. Fine mapping using the lambda 1, lambda 3 rearranged cell line J558 mapped the gap between the V and J-C gene segments in the lambda 1 gene cluster (V1-J3C3J1C1) to approximately 70 kb. The similar distance (60-100 kb) found in the lambda 2 gene cluster (V2-J2C2J4C4) is further evidence that duplication of an ancestral locus occurred.
authors.affiliation: Basel Institute for Immunology, Switzerland.
authors.forename: S
authors.initials: S
authors.lastname: Carson
authors.forename: G E
authors.initials: GE
authors.lastname: Wu
mesh_terms: D000818:Animals; D002460:Cell Line; D002874:Chromosome Mapping; D004587:Electrophoresis, Agar Gel; D005803:Genes, Immunoglobulin; D008040:Genetic Linkage; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics; D051379:Mice / Q000235:genetics*; D015183:Restriction Mapping
chemical_list: D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region