Determination of opiates and other basic drugs by high-performance liquid chromatography with electrochemical detection.
A procedure is described for the extraction and determination of morphine (M), hydromorphone (HM), codeine (C) and metoclopramide (MCP) present in human plasma. The drugs are separated by reversed-phase liquid chromatography and detected amperometrically at a glassy carbon electrode. The method provides high sensitivity and selectivity and has been used successfully in bioavailability studies.
Besner
J G
JG
University of Montreal, Faculty of Pharmacy, Quebec, Canada.
Band
C
C
Rondeau
J J
JJ
Yamlahi
L
L
Caillé
G
G
Varin
F
F
Stewart
J
J
D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid; D003061:Codeine / Q000032:analysis / Q000493:pharmacokinetics; D004563:Electrochemistry; D004566:Electrodes; D006801:Humans; D007202:Indicators and Reagents; D008787:Metoclopramide / Q000032:analysis / Q000493:pharmacokinetics; D009020:Morphine / Q000032:analysis / Q000493:pharmacokinetics; D009294:Narcotics / Q000032:analysis* / Q000493:pharmacokinetics; D010084:Oxidation-Reduction; D012015:Reference Standards
D007202:Indicators and Reagents; D009294:Narcotics; D009020:Morphine; D008787:Metoclopramide; D003061:Codeine