Relationship between cell kinetics and radiation-induced arrest of proliferating cells in G2: relevance to efficacy of radiotherapy.
Monitoring the radiation-induced G2-arrest with flow cytometry disclosed the relationship between the pattern of accumulation in G2 and the cell kinetics (Tc), as assessed with semi-continuous labelling. A close relationship between these two parameters was observed in a murine mammary tumour after high dose-rate irradiation and in human cervix tumours (xenografted and in patients) after low dose-rate irradiation. In inoperable tumours of the oral cavity, a G2-arrest, observed in the first two weeks of fractionated therapy, was found to predict a local cure. Consequently, it was concluded that the cells accumulated in G2 were not doomed cells. This process of radiation-induced synchronization might be exploited in fractionation schemes of which treatment intervals are adjusted to the Tc-related timing of the radiation-induced G2-arrest.
Rutgers
D H
DH
Department of Radiotherapy, University Hospital, Utrecht, The Netherlands.
van Oostrum
I E
IE
Noorman van der Dussen
M F
MF
Wils
I S
IS
D000818:Animals; D001918:Brachytherapy; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy / Q000473:pathology / Q000532:radiotherapy*; D002453:Cell Cycle / Q000528:radiation effects*; D002455:Cell Division / Q000528:radiation effects; D002588:Cesium Radioisotopes / Q000627:therapeutic use; D003131:Combined Modality Therapy; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007399:Interphase / Q000528:radiation effects; D008325:Mammary Neoplasms, Experimental / Q000473:pathology / Q000532:radiotherapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D009062:Mouth Neoplasms / Q000473:pathology / Q000532:radiotherapy*; D009368:Neoplasm Transplantation; D011836:Radiation Tolerance; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured / Q000528:radiation effects; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy / Q000473:pathology / Q000532:radiotherapy*
D002588:Cesium Radioisotopes; D004273:DNA, Neoplasm