Effect of donation time on platelet concentrates and fresh-frozen plasma. An in vitro study.
To investigate the effect of donation time on the quality of blood components, we measured the platelet count and pH on platelet concentrates, and the factor V and VIII:C levels and fibrinopeptide A concentration on fresh-frozen plasma by duration of donation time. Platelet concentrates and fresh-frozen plasma were classified into three groups according to donation time: group 1, less than 10 min; group 2, 10-15 min, and group 3, longer than 15 min. Mean platelet counts of platelet concentrate were: group 1, 8.6 +/- 2.5 (in x 10(10], group 2, 8.1 +/- 2.6, and group 3, 6.5 +/- 3.2 (p less than 0.05). The same pH was maintained in all three groups. The fibrinopeptide A concentrations in groups 1 and 2 were 24 +/- 53 and 169 +/- 64 ng/ml, respectively, while in group 3 all were greater than 200 ng/ml, indicating correlation of a higher fibrinopeptide A level with longer donation time. Although a higher fibrinopeptide A level indicated a greater degree of thrombin generation, assays of factors V and VII:C did not show decreased activity in groups 2 and 3.
Huh
Y O
YO
University of Texas, M.D. Anderson Cancer Center at Houston.
Lichtiger
B
B
Giacco
G G
GG
Guinee
V F
VF
Drewinko
B
B
D000941:Antigens / Q000032:analysis; D001792:Blood Platelets; D001800:Blood Specimen Collection; D005165:Factor V / Q000032:analysis; D005169:Factor VIII / Q000032:analysis; D005344:Fibrinopeptide A / Q000032:analysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010949:Plasma; D010976:Platelet Count; D013997:Time Factors
D000941:Antigens; D005344:Fibrinopeptide A; D005165:Factor V; D005169:Factor VIII