Demonstration of GTP-binding proteins and ADP-ribosylated proteins in rat liver Golgi fraction.
A Golgi-rich fraction isolated from rat liver was found to contain GTP-binding proteins with 20-25 kDa, which were tightly bound to the Golgi membrane. The Golgi fraction also contained two species of proteins which were ADP-ribosylated by bacterial toxins. Protein(s) which was ADP-ribosylated by botulinum toxin had a similar molecular mass as those with GTP-binding activity but was easily released from the membrane. Another protein with 46 kDa which was ADP-ribosylated by pertussis toxin was tightly bound to the membrane but had no significant GTP-binding activity under conditions tested here. These proteins were much less or negligible in the plasma membrane and the endoplasmic reticulum.
Toki
C
C
Department of Biochemistry, Fukuoka University School of Medicine, Japan.
Oda
K
K
Ikehara
Y
Y
D000818:Animals; D001905:Botulinum Toxins / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism*; D037342:Pertussis Toxin; D011064:Poly Adenosine Diphosphate Ribose / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D010566:Virulence Factors, Bordetella / Q000378:metabolism
D009702:Nucleoside Diphosphate Sugars; D011506:Proteins; D010566:Virulence Factors, Bordetella; D011064:Poly Adenosine Diphosphate Ribose; D037342:Pertussis Toxin; D001905:Botulinum Toxins; D019204:GTP-Binding Proteins