title: Lipoxygenase activities of the epithelial cells of the human buccal cavity.
abstract: The lipoxygenase activities of cultured human buccal epithelial cells and cells taken ex vivo from the human buccal cavity were compared. Lipoxygenation by cultured cells exhibited exclusively omega-6 positional specificity. A membrane-damaging event such as freezing was required for activation. In contrast, after simple addition of arachidonic acid, the ex vivo buccal cells produced predominantly 12-hydroxyeicosatetraenoic acid. Chromatography on chiral columns yielded products which had an (S) configuration at the site of oxygen insertion. 5-hydroxyeicosatetraenoic acid was transformed by ex vivo buccal cells to 5(S),12(S)-dihydroxyeicosatetraenoic acid. These products could have a role in modulation of inflammatory states of the oral cavity.
authors.affiliation: Department of Medicine, State University of New York, Buffalo.
authors.forename: F A
authors.initials: FA
authors.lastname: Green
mesh_terms: D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D016718:Arachidonic Acid; D001095:Arachidonic Acids; D002454:Cell Differentiation; D002478:Cells, Cultured; D002610:Cheek / Q000201:enzymology* / Q000378:metabolism / Q000502:physiology; D002849:Chromatography, Gas; D004848:Epithelium / Q000201:enzymology / Q000378:metabolism / Q000502:physiology; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis; D008084:Lipoxygenase / Q000378:metabolism*
chemical_list: D001095:Arachidonic Acids; D006893:Hydroxyeicosatetraenoic Acids; D016718:Arachidonic Acid; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid; D008084:Lipoxygenase