Serologic and immunocytochemical analysis of the Mycobacterium avium cell envelope.
Whole cell sonicates of Mycobacterium avium ATCC 15769, and subcellular fractions (CYT, cytosol; CM, cytoplasmic membrane; CWS, delipidated cell wall skeleton; OL, native lipids; and SDS, sodium dodecylsulfate extract of whole cells) were injected into rabbits to produce corresponding antisera. Immunoelectrophoretic analysis and immunochemical observations using electron microscopy showed that few of the antigens synthetized intracellularly were exported and located in the bacterial outer layers, and that the outer layers contained wall specific antigens possibly in situ assembled.
David
H L
HL
Unité de la Tuberculose et des Mycobactéries, Institut Pasteur, Paris, France.
Thorel
M F
MF
Fréhel
C
C
Rastogi
N
N
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D002473:Cell Wall / Q000276:immunology; D007150:Immunohistochemistry; D009162:Mycobacterium avium / Q000276:immunology*; D013347:Subcellular Fractions / Q000276:immunology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial