title: Mutations that alter the helix-turn-helix region of the spollAC protein: a Bacillus subtilis sporulation-specific sigma factor.
abstract: It has previously been shown that spollAC561, a mutation that diminishes the incidence of sporulation by more than six orders of magnitude, alters the residue at position 13 of the helix-turn-helix region of the sporulation-specific sigma factor encoded by spollAC from valine to methionine (Yudkin, 1987b). We have now found that four spontaneous revertants, which sporulate at an incidence of 30-60%, all contain transitions within the codon that was altered by spo-561. The mutant methionine is replaced by isoleucine in two revertants, and by threonine in the other two.
authors.affiliation: Department of Biochemistry, University of Oxford, UK.
authors.forename: M D
authors.initials: MD
authors.lastname: Yudkin
authors.forename: J H
authors.initials: JH
authors.lastname: Millonig
authors.forename: L
authors.initials: L
authors.lastname: Appleby
mesh_terms: D000596:Amino Acids / Q000032:analysis; D001412:Bacillus subtilis / Q000235:genetics* / Q000502:physiology; D001426:Bacterial Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D003062:Codon; D004252:DNA Mutational Analysis; D004269:DNA, Bacterial; D011487:Protein Conformation; D015183:Restriction Mapping; D012808:Sigma Factor / Q000235:genetics*; D013171:Spores, Bacterial / Q000254:growth & development; D013489:Suppression, Genetic; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; D003062:Codon; D004269:DNA, Bacterial; D012808:Sigma Factor; D014157:Transcription Factors