title: The structure of the aflatoxin B1-DNA adduct at N7 of guanine. Theoretical intercalation and covalent adduct models.
abstract: Two theoretical models are proposed for the conformational structure of both intercalated and covalent adduct complexes of aflatoxin B1, designated AFB1, with N7 of guanine of DNA. The covalent adduct model requires the DNA to kink a minimum of 39 degrees about the covalent site of the C8 and N7 atoms comprising the bond of the covalent complex. The preference of AFB1 for specific G bases within a sequence of GC content followed that of experimental studies with the added feature that for binding to the third G base of a tetramer sequence from the 3'-end, the AFB1 displayed enhanced binding at the 3' site of the targeted guanosine. Binding of AFB1 to the second G base of a tetramer sequence from the 3'-end leads to preference for a 5' site of the targeted guanosine. Inhibition of AFB1's interaction with the targeted DNA in the presence of intercalated ethidium bromide is explained by these proposed models.
authors.affiliation: Department of Chemistry, University of Southwestern Louisiana, Lafayette 70504-4370.
authors.forename: M
authors.initials: M
authors.lastname: Bonnett
authors.forename: E R
authors.initials: ER
authors.lastname: Taylor
mesh_terms: D016604:Aflatoxin B1; D000348:Aflatoxins / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D006147:Guanine; D007364:Intercalating Agents / Q000493:pharmacokinetics*; D008958:Models, Molecular; D009690:Nucleic Acid Conformation
chemical_list: D000348:Aflatoxins; D007364:Intercalating Agents; D006147:Guanine; D004247:DNA; D016604:Aflatoxin B1