Further resolution of the quail karyotype and characterization of microchromosomes by counterstain-enhanced fluorescence.
Sequential staining with a counterstain-contrasted fluorescent R-banding technique (chromomycin A3/distamycin A-DAPI) followed by DAPI-actinomycin D-induced quinacrine-fluorescence-Hoechst 33258 (QFH)-type banding allowed the identification of quail chromosomes up to chromosome 19. The chromomycin A3-positive staining behavior of the W chromosome and of the heterochromatic areas of most microchromosomes indicated their GC-rich nature.
Mayr
B
B
Institute for Animal Breeding and Genetics, Veterinary University, Vienna, Austria.
Lambrou
M
M
Schleger
W
W
D000818:Animals; D014128:Chromomycin A3; D002871:Chromosome Banding; D003370:Coturnix / Q000235:genetics*; D004214:Distamycins; D005260:Female; D005456:Fluorescent Dyes; D007211:Indoles; D007621:Karyotyping / Q000379:methods*; D008297:Male; D011784:Quail / Q000235:genetics*
D004214:Distamycins; D005456:Fluorescent Dyes; D007211:Indoles; C007293:DAPI; C030000:stallimycin; D014128:Chromomycin A3