[Use of immunospecific methods for quantitative estimation of microsomal epoxide hydrolase].
Antiserum, specific to rat liver microsomal epoxide hydrolase, was produced. Three methods for quantitative estimation of the epoxide hydrolase, base on the principle of antigen-antiserum immunoprecipitation, were used: electroimmune analysis by Laurell, radial immunodiffusion, enzyme-linked immunoassay. Distinct correlation between specific enzyme activity and its content in microsomal preparation was demonstrated using a simple and stable procedure of radial immunodiffusion. In the enzyme-linked assay conjugate of protein A with alkaline phosphate served as the secondary antibody. Sensitivity of the procedure described was about 1,000-fold higher as compared with radial immunodiffusion method.
D'iakov
V L
VL
Dubovskoĭ
A V
AV
Klimova
O A
OA
Sukhanov
V A
VA
D000818:Animals; D002138:Calibration; D004797:Enzyme-Linked Immunosorbent Assay; D004851:Epoxide Hydrolases / Q000378:metabolism*; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D008862:Microsomes, Liver / Q000201:enzymology*; D011817:Rabbits; D051381:Rats
D004851:Epoxide Hydrolases
Ispol'zovanie immunospetsificheskikh metodov dlia opredeleniia kolichestva mikrosomal'noĭ époksidgidrolazy.