title: ADH activity and ethanol tolerance in third chromosome substitution lines in Drosophila melanogaster.
abstract: In vitro ADH activity and ethanol tolerance were studied in males of a series of third chromosome substitution lines in Drosophila melanogaster. The lines were divided into those with a random third chromosome from a vineyard population (VO lines) and those with a selected third chromosome from males obtained after an egg-to-adult ethanol survival test on the F4 of the previous population (VE lines). Both ADH activity and ethanol tolerance varied significantly among the lines, but the characters showed no significant correlation. Ethanol tolerance (at the higher ethanol concentrations) was higher in the selected lines (VE lines) but ADH activity was not. In our lines, the in vitro ADH activity variability, linked to the regulatory genes (located on the third chromosome) and unrelated to the polymorphism of the Adh locus (located on the second chromosome), is not involved in the ethanol tolerance variability. The data suggest that in this population ethanol tolerance was acquired in nature, at least partially, by means other than increasing ADH activity.
authors.affiliation: Laboratoire de Génétique des Populations Universités Paris VI & VII, France.
authors.forename: H
authors.initials: H
authors.lastname: Merçot
authors.forename: L
authors.initials: L
authors.lastname: Massaad
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D000483:Alleles; D000818:Animals; D002875:Chromosomes; D004331:Drosophila melanogaster / Q000235:genetics*; D004361:Drug Tolerance; D000431:Ethanol / Q000494:pharmacology*; D014644:Genetic Variation; D008297:Male; D009026:Mortality
chemical_list: D000431:Ethanol; D000426:Alcohol Dehydrogenase