The application of S. mansoni adult and soluble egg antigens for the serodiagnosis of schistosomiasis by CIEP, IHA and ELISA.
The sero-reactivity of 82 schistosomiasis infected patients were studied by the use of the CIEP, IHA and ELISA tests using S. mansoni adult worm antigen (AWA) and soluble egg antigen (SEA). CIEP with (AWA) showed a progressive sensitivity decreased with advance of S. mansoni infection, and increased in sensitivity by (SEA). The test was more sensitive in the early cases of S. haematobium infection by (AWA), while by (SEA) it was more sensitive in the late cases. There was no difference in sensitivity of the test with either antigens in mixed bilharzial infection. IHAT with (AWA) showed high sensitivity in the different stages of S. mansoni infection, but sensitivity decreased with the progress of clinical status of S. mansoni if (SEA) was utilized. The test with (AWA) was slightly more sensitive in the early cases of S. haematobium than the late cases, while the same high sensitivity was given by the test with (SEA) in both groups. Also IHAT with (AWA) was more sensitive for the diagnosis of mixed bilharzial infection than with (SEA). ELISA test with (SEA) showed a progressive increase in sensitivity with the advance of S. mansoni. Also the test with (SEA) was more sensitive than with (AWA); and for the diagnosis of cases of mixed bilharzial infection, while S. haematobium infected cases showed a lower sensitivity than S. mansoni or mixed bilharzial infection cases, if the ELISA test is performed with either antigens.
Khalil
H M
HM
Makled
M K
MK
Elmissiry
A G
AG
Khalil
N M
NM
Sonbol
S E
SE
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D003378:Counterimmunoelectrophoresis; D004797:Enzyme-Linked Immunosorbent Assay; D006386:Hemagglutination Tests; D006801:Humans; D012550:Schistosoma mansoni / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000175:diagnosis*; D012995:Solubility
D000947:Antigens, Helminth