Analysis of structural and biological parameters affecting plasmid deletion formation in Bacillus subtilis.
Deletions generated following stimulation by the deletion hot spot of plasmid pHV15-1 were studied in Bacillus subtilis. Nucleotide sequencing showed that deletions extend between short direct repeat sequences. Such direct repeat sequences may have homology to the sequence of the hot spot. Deletion formation is recE-independent, but requires an active exonuclease V (AddAB) enzyme. Other structural parameters like plasmid size and structure influence deletion formation.
Kupsch
J
J
Max-Planck-Institut für Molekulare Genetik, Berlin.
Alonso
J C
JC
Trautner
T A
TA
D001412:Bacillus subtilis / Q000235:genetics*; D001483:Base Sequence; D002872:Chromosome Deletion; D004261:DNA Replication; D005810:Multigene Family; D010957:Plasmids; D011995:Recombination, Genetic; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014161:Transduction, Genetic