title: Detection of intracytoplasmic immunoglobulin by flow cytometry in B-cell malignancies.
abstract: The aim of this study was to compare the results of flow cytometric (FCM) determination of heavy and light chain cytoplasmic immunoglobulin (cIg) with those obtained by the peroxidase-antiperoxidase (PAP) method. Fifty-one patients, including five non-T-acute lymphoblastic leukemias, 16 B-chronic lymphocytic leukemias (CLL), 13 non-Hodgkin's lymphomas, seven hairy cell leukemias, four multiple myeloma/plasma cell leukemias, and six T-cell leukemia/lymphomas, as well as 12 normal controls, were studied. Saponin-permeabilized cell suspensions were indirectly stained with monoclonal antibodies and analyzed by flow cytometry. Acetone-fixed cytocentrifuge smears were stained for cIg by the PAP method. The results obtained indicate that: (a) detection of cIg by FCM is a feasible and useful technique to confirm the B-cell lineage of leukemias and lymphomas, particularly those characterized by low-density surface immunoglobulin, such as CLL; and (b) cIg detection by FCM and PAP staining are complementary methods to recognize with certainty the monoclonality of B-cell malignancies.
authors.affiliation: Lymphocyte Surface Markers Laboratory, Memorial Sloan-Kettering Cancer Center, New York, New York 10021.
authors.forename: R H
authors.initials: RH
authors.lastname: Bardales
authors.forename: A M
authors.initials: AM
authors.lastname: Al-Katib
authors.forename: A
authors.initials: A
authors.lastname: Carrato
authors.forename: B
authors.initials: B
authors.lastname: Koziner
mesh_terms: D001402:B-Lymphocytes / Q000276:immunology*; D003593:Cytoplasm / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D007124:Immunoenzyme Techniques; D007143:Immunoglobulin Heavy Chains / Q000032:analysis; D007147:Immunoglobulin Light Chains / Q000032:analysis; D007136:Immunoglobulins / Q000032:analysis*; D007938:Leukemia / Q000276:immunology*; D007943:Leukemia, Hairy Cell / Q000276:immunology; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000276:immunology; D007952:Leukemia, Plasma Cell / Q000276:immunology; D015458:Leukemia, T-Cell / Q000276:immunology; D008223:Lymphoma / Q000276:immunology*; D009101:Multiple Myeloma / Q000276:immunology*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology
chemical_list: D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007136:Immunoglobulins