Characterization and purification of a placental protein that inactivates GnRH, TRH and angiotensin II.
A protein that inactivates the immunoreactivity of GnRH, TRH and angiotensin II has been isolated from human term placentae. Only in the presence of DTT, a sulphydryl agent, are OXY and SRIF also inactivated by this protein. However, it is without effect on CRF, hCS, or hCG. It also inhibits the biological activity of GnRH, i.e. its ability to stimulate pituitary LH and FSH. The ability of this protein to inactivate GnRH, TRH or angiotensin II can be inhibited by various peptidase inhibitors. Thus, we have postulated that it is a chorionic peptidase, specific for these peptides, and herein called chorionic peptidase-1 (C-ase-1). Isolation of this protein, C-ase-1, has been effected using permeation, ion exchange and affinity chromatography. As estimated by SDS-PAGE and HPLC analyses, C-ase-1 has an apparent molecular weight of 58,000. It is proposed that C-ase-1 may be an important chorionic regulator of GnRH, TRH and angiotensin II levels during pregnancy.
Siler-Khodr
T M
TM
Department of Obstetrics and Gynaecology, University of Texas, Health Science Center at San Antonio 78284.
Kang
I S
IS
Jones
M A
MA
Harper
M J
MJ
Khodr
G S
GS
Rhode
J
J
D000804:Angiotensin II / Q000037:antagonists & inhibitors*; D001681:Biological Assay; D002824:Chorionic Villi / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000032:analysis* / Q000302:isolation & purification; D005260:Female; D006801:Humans; D010447:Peptide Hydrolases / Q000032:analysis* / Q000302:isolation & purification; D010906:Pituitary Hormone-Releasing Hormones / Q000037:antagonists & inhibitors*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000032:analysis* / Q000302:isolation & purification; D011863:Radioimmunoassay; D013973:Thyrotropin-Releasing Hormone / Q000037:antagonists & inhibitors*
D010906:Pituitary Hormone-Releasing Hormones; D011257:Pregnancy Proteins; D000804:Angiotensin II; D013973:Thyrotropin-Releasing Hormone; D010450:Endopeptidases; D010447:Peptide Hydrolases; C060922:chorionic peptidase I