Purification of xylanase, beta-glucosidase, endocellulase, and exocellulase from a thermophilic fungus, Thermoascus aurantiacus.
A strain of thermophilic fungus, Thermoascus aurantiacus, was isolated from local soil. From the culture filtrates of the organism grown on blotting paper, a xylanase, beta-glucosidase, exocellulase, and endocellulase were obtained in large amounts in highly purified form by employing ion-exchange and gel-permeation chromatography. The xylanase was crystallized. The xylanase and endocellulase were stable at 70 degrees C for 8 h, whereas the beta-glucosidase and exocellulase were less stable at 70 degrees C.
Khandke
K M
KM
Department of Biochemistry, Indian Institute of Science, Bangalore.
Vithayathil
P J
PJ
Murthy
S K
SK
D001203:Ascomycota / Q000201:enzymology* / Q000254:growth & development; D002241:Carbohydrates / Q000032:analysis; D002244:Carbon; D043366:Cellulose 1,4-beta-Cellobiosidase; D003460:Crystallization; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability; D005110:Extracellular Space / Q000201:enzymology; D005656:Fungal Proteins / Q000096:biosynthesis / Q000302:isolation & purification*; D005959:Glucosidases / Q000302:isolation & purification*; D006026:Glycoside Hydrolases / Q000096:biosynthesis / Q000302:isolation & purification*; D006358:Hot Temperature; D043325:Xylan Endo-1,3-beta-Xylosidase; D001617:beta-Glucosidase / Q000096:biosynthesis / Q000302:isolation & purification*
D002241:Carbohydrates; D005656:Fungal Proteins; D002244:Carbon; D005959:Glucosidases; D006026:Glycoside Hydrolases; D001617:beta-Glucosidase; D043325:Xylan Endo-1,3-beta-Xylosidase; D043366:Cellulose 1,4-beta-Cellobiosidase