In vivo regeneration of red cell 2,3-diphosphoglycerate following transfusion of DPG-depleted AS-1, AS-3 and CPDA-1 red cells.
Regeneration of 2,3-diphosphoglycerate (DPG) was determined following transfusion of DPG-depleted group O red cells into group A recipients. Blood from five donors was stored in the adenine-containing solutions CPDA-1, AS-1 or AS-3 for 35 d at 4 degrees C. Post-transfusion red cell DPG and ATP were measured in separated group O red cells over a 7 d period. The studies confirmed rapid in vivo DPG regeneration with greater than or equal to 50% of the maximum level being achieved within 7 h. An average of 95% of the recipients' pre-transfusion DPG level was achieved by 72 h and by 7 d mean (+/- SEM) DPG levels relative to recipient's pre-transfusion DPG averaged 84% (+/- 13%), 92% (+/- 17%) and 84% (+/- 21%) for CPDA-1, AS-1 and AS-3 red cells, respectively. Results were comparable to those previously reported for blood stored in ACD for 15-20 d (Valeri & Hirsch, 1969; Beutler & Wood, 1969). The immediate regeneration rate, V, closely approximated first order regeneration kinetics with AS-3 red cells exhibiting double the rate of CPDA-1 red cells (P less than 0.001). AS-1 red cells exhibited an intermediate rate of regeneration which was not significantly different compared to either CPDA-1 or AS-3 (P greater than 0.05). V exhibited a significant (P less than 0.05) positive correlation with ATP levels 5-7 h post-infusion. ATP regeneration of the infused cells was rapid with a mean increase of 1.2 mumol/g Hb above post-storage levels being achieved 1 h following transfusion.
Heaton
A
A
Tidewater Red Cross Blood Services, Norfolk, VA 23507.
Keegan
T
T
Holme
S
S
D019794:2,3-Diphosphoglycerate; D000225:Adenine; D000328:Adult; D000925:Anticoagulants; D001793:Blood Preservation; D001803:Blood Transfusion; D002951:Citrates; D004163:Diphosphoglyceric Acids / Q000097:blood*; D017707:Erythrocyte Transfusion; D004912:Erythrocytes / Q000378:metabolism*; D005947:Glucose; D006801:Humans; D008297:Male; D008353:Mannitol; D010710:Phosphates; D012965:Sodium Chloride; D012996:Solutions
C037839:ADSOL; C058647:AS-3 preservative solution; D000925:Anticoagulants; C034876:CPDA solutions; D002951:Citrates; D004163:Diphosphoglyceric Acids; D010710:Phosphates; D012996:Solutions; D019794:2,3-Diphosphoglycerate; D008353:Mannitol; D012965:Sodium Chloride; D005947:Glucose; D000225:Adenine