[New cloning vectors constructed in Bacillus].
Two new plasmids pNQ216 (4.1kp) and pNQ402 (2.8 kb) were constructed by combining the replication origin of the plasmid pNK289, a cryptic plasmid resides in B. pumilus 289, and the cat-86 gene from plasmid pPL601. These two plasmids can be maintained steadily in both B. subtilis and B. pumilus. The penetrance of these plasmids on LB medium with Cm (20 micrograms/ml) is 30% higher than that of pPL600. Thus both of these plasmids can be used as new cloning vectors in Bacillus.
Qiao
M
M
Cai
B
B
Gao
C
C
Lang
Y
Y
Fan
T
T
Su
T F
TF
Jiang
R
R
D001407:Bacillus / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors; D010957:Plasmids