title: Disruption of the optic pathway during development affects vasoactive intestinal peptide mRNA expression.
abstract: Vasoactive intestinal polypeptide (VIP) is a regulatory peptide widely distributed in the central and peripheral nervous systems. To understand the activities of VIP it is necessary to study the mechanisms governing its production. The highest concentration of VIP-producing cells occurs in the suprachiasmatic nucleus (SCN) of the hypothalamus. Because the SCN is directly innervated by the optic nerve, we decided to investigate the effect of visual input on VIP gene expression. By means of Northern blot hybridization, we measured VIP mRNA levels in the hypothalami of 36-day-old normal rats and rats that had been enucleated at birth. The concentration of VIP mRNA in the hypothalami of enucleated rats was approximately double that in the hypothalami of normal rats. In contrast, the concentration of VIP mRNA in the cerebral cortex significantly decreased after enucleation. The concentrations of VIP mRNA were also measured by in situ hybridization to brain sections. The hypothalamic VIP mRNA was located mainly in the SCN. Enucleation resulted in an increase of VIP transcripts in the SCN. These results indicate that visual input may participate in the regulation of VIP production.
authors.affiliation: Department of Hormone Research, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: R L
authors.initials: RL
authors.lastname: Holtzman
authors.forename: R
authors.initials: R
authors.lastname: Malach
authors.forename: I
authors.initials: I
authors.lastname: Gozes
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000737:chemistry / Q000254:growth & development; D015353:Eye Enucleation; D005786:Gene Expression Regulation; D007031:Hypothalamus / Q000737:chemistry / Q000254:growth & development; D008297:Male; D009900:Optic Nerve / Q000502:physiology; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D013493:Suprachiasmatic Nucleus / Q000378:metabolism*; D014660:Vasoactive Intestinal Peptide / Q000096:biosynthesis* / Q000235:genetics; D014795:Visual Pathways / Q000254:growth & development / Q000293:injuries / Q000502:physiology*
chemical_list: D015347:RNA Probes; D012333:RNA, Messenger; D014660:Vasoactive Intestinal Peptide