Degradation of basement membranes by Pseudomonas aeruginosa elastase.
Pseudomonas aeruginosa virulence has been attributed in part to extracellular proteinases. We found that one of these proteinases, elastase, extensively degrades intact basement membranes from bovine anterior-lens capsules, bovine glomeruli, and bovine lung, producing about 9, 14, and 9 fragments, respectively, with Mrs in the range of 15,000 to greater than 200,000, as shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (nonreducing conditions). Release of hydroxyproline showed that collagen IV was degraded by elastase. Degradation of the newly discovered alpha 3(IV) collagen chain was shown by immunoblotting of digests with Goodpasture's syndrome serum, which contains antibodies that react with an epitope located in the carboxyl-terminal globular (NCl) domain of alpha 3(IV). Comparison of total protein release with collagen IV release showed that noncollagenous protein components were solubilized to the same extent as collagen IV. The extensive degradation of the basement membranes described here suggests a role for elastase in the pathogenic mechanism at the local level when P. aeruginosa infection is present.
Bejarano
P A
PA
Department of Biochemistry, University of Kansas Medical Center, Kansas City 66103.
Langeveld
J P
JP
Hudson
B G
BG
Noelken
M E
ME
D000818:Animals; D001485:Basement Membrane / Q000378:metabolism*; D002417:Cattle; D003094:Collagen / Q000378:metabolism; D066298:In Vitro Techniques; D007678:Kidney Glomerulus / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008970:Molecular Weight; D010196:Pancreatic Elastase / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D013997:Time Factors
D003094:Collagen; D010196:Pancreatic Elastase