Local murine recombinant gamma interferon enhances the acquisition of allergic contact dermatitis in the mouse.
Using the model of allergic contact dermatitis in mice, we have demonstrated that local administration of recombinant (r) murine gamma interferon (gamma-rIFN) to the sensitization site substantially enhances the acquisition of delayed-type hypersensitivity. Single doses of gamma-rIFN from 500 to 2,000 U immunopotentiate successfully, with a suggestion of a better response at the higher doses. The immunoadjuvant effect is lost when the injections of gamma-rIFN are at a site distant from the sensitization site. Kinetic studies imply that gamma-rIFN is most effective when given at the time of sensitization, but significant immunopotentiation can be seen when gamma-rIFN is given several days before or after sensitization.
Maguire
H C
HC
Department of Medicine, Hahnemann University School of Medicine, Philadelphia, Pa.
Guidotti
M
M
Weidanz
W P
WP
D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D003877:Dermatitis, Contact / Q000209:etiology* / Q000276:immunology; D004139:Dinitrofluorobenzene / Q000008:administration & dosage; D005260:Female; D007114:Immunization; D007271:Injections, Intradermal; D007371:Interferon-gamma / Q000008:administration & dosage*; D051379:Mice; D008807:Mice, Inbred BALB C; D010081:Oxazolone / Q000008:administration & dosage; D011994:Recombinant Proteins
D000276:Adjuvants, Immunologic; D011994:Recombinant Proteins; D010081:Oxazolone; D007371:Interferon-gamma; D004139:Dinitrofluorobenzene