title: Glycosaminoglycan expression in intestinal epithelial skin-fibroblastic cell cocultures. Fibroblastic cell-mediated effects of glucocorticoids.
abstract: The nature and distribution of newly synthesized glycosaminoglycans (GAGs) were studied in foetal rat skin fibroblasts, in rat intestinal endodermal cells and in cocultures of both cell types. The data show that fibroblasts synthesize and secrete hyaluronic acid (HA), heparan sulphate (HS) and chondroitin sulphate molecules (CS). Our data focus on HA, which is found as two different molecular forms, the smallest hydrodynamic-sized species being mostly recovered within the cell or associated with the cell surface, and the largest one secreted into the medium, whatever the cell type. Endodermal cells synthesize only two types of GAGs: the low molecular weight form of HA and HS. Cocultures of rat intestinal endodermal and skin fibroblastic cells in the presence of dexamethasone (Dx), allow optimal epithelial cytodifferentiation (Kedinger et al. 1987a). The main changes in the GAGs synthesized under these conditions as compared to skin fibroblastic cell cultures concern: (1) the enhancement of the lowest molecular weight form of HA to the detriment of the highest form in the cellular, pericellular and extracellular compartments; (2) the increase in the proportion of HS molecules associated with the cell surface. Interestingly, similar modifications are obtained by addition of Dx to the skin fibroblastic cell cultures. The data are discussed with reference to the constitution of a basement membrane at the epithelial-fibroblast interface in the cocultures, to the fibroblastic-dependent induction of epithelial differentiation and to the glucocorticoid response.
authors.affiliation: INSERM Unité 61, Biologie Cellulaire et Physiopathologie Digestives, Strasbourg-Hautepierre, France.
authors.forename: F
authors.initials: F
authors.lastname: Bouziges
authors.forename: P
authors.initials: P
authors.lastname: Simon-Assmann
authors.forename: C
authors.initials: C
authors.lastname: Leberquier
authors.forename: K
authors.initials: K
authors.lastname: Haffen
authors.forename: M
authors.initials: M
authors.lastname: Kedinger
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000096:biosynthesis; D002852:Chromatography, Ion Exchange; D003907:Dexamethasone / Q000494:pharmacology*; D004707:Endoderm / Q000166:cytology / Q000378:metabolism*; D004848:Epithelium; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D006497:Heparan Sulfate / Q000096:biosynthesis; D006820:Hyaluronic Acid / Q000096:biosynthesis; D007422:Intestines; D051381:Rats; D012867:Skin
chemical_list: D002809:Chondroitin Sulfates; D003907:Dexamethasone; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D006820:Hyaluronic Acid