title: Liquid chromatographic determination of penicillin V potassium in tablets: collaborative study.
abstract: A liquid chromatographic method for the determination of penicillin V potassium in tablets was collaboratively studied by 7 laboratories. The method uses an octadecyl silane reverse-phase column, a mobile phase of acetonitrile-methanol-0.01 M potassium phosphate monobasic (21 + 4 + 75 v/v/v), photometric detection at 225 nm, and sulfadimethoxine as an internal standard. Each collaborator received 6 samples: powdered composites of 2 commercial tablet preparations and 1 synthetic tablet powder mixture, each with blind duplicates. The mean repeatability and reproducibility relative standard deviations for commercial samples were, respectively, 1.10 and 1.46% (250 mg dosage), and 0.84 and 2.82% (500 mg dosage). The average standard recovery from the synthetic formulation was 99.1%, with repeatability and reproducibility relative standard deviations of 1.30 and 3.66%, respectively. The method has been adopted official first action.
authors.affiliation: Food and Drug Administration, New York Regional Laboratory, Brooklyn 11232-1593.
authors.forename: B
authors.initials: B
authors.lastname: Mopper
mesh_terms: D002853:Chromatography, Liquid; D007202:Indicators and Reagents; D010404:Penicillin V / Q000032:analysis*; D013607:Tablets
chemical_list: D007202:Indicators and Reagents; D013607:Tablets; D010404:Penicillin V