title: 31P-NMR spectroscopy of phosphate compartmentation during ischaemia in hearts protected by cardioplegic treatment.
abstract: Four tissue compartments, differing in proton and inorganic phosphate concentration, were resolved by 31P-NMR spectroscopy in samples from dog hearts after cardioplegic treatment with HTK solution. Inversion of the physiological cytoplasmic-mitochondrial pH gradient was observed. The considerable ensuing acidosis of the matrix is discussed with regard to a possible delocalization of ferrous ions.
authors.affiliation: Max-Planck-Institut für Biophysikalische Chemie, Göttingen, W. Germany.
authors.forename: A
authors.initials: A
authors.lastname: Stier
authors.forename: S A
authors.initials: SA
authors.lastname: Finch
authors.forename: H
authors.initials: H
authors.lastname: Schäfer
authors.forename: M M
authors.initials: MM
authors.lastname: Gebhardt
authors.forename: H J
authors.initials: HJ
authors.lastname: Bretschneider
mesh_terms: D000818:Animals; D002314:Cardioplegic Solutions / Q000494:pharmacology*; D003327:Coronary Disease / Q000378:metabolism*; D004285:Dogs; D005947:Glucose / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D008353:Mannitol / Q000494:pharmacology; D009206:Myocardium / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism*; D011189:Potassium Chloride / Q000494:pharmacology; D011343:Procaine / Q000494:pharmacology
chemical_list: C039984:Bretschneider cardioplegic solution; D002314:Cardioplegic Solutions; D010710:Phosphates; D008353:Mannitol; D011343:Procaine; D011189:Potassium Chloride; D005947:Glucose