Leukotriene C4 formation by purified human eosinophils can be induced by arachidonic acid in the absence of calcium-ionophore A23187.
Addition of arachidonic acid (50 microM) to purified human eosinophils leads to the formation of considerable amounts of LTC4 [11.3 +/- 1.3) x 10(6) molecules/cell, mean +/- SEM, n = 10), 15-HETE [412 +/- 142) x 10(6) molecules/cell, mean +/- SEM, n = 3) and 15-series leukotrienes [35 +/- 15) x 10(6) molecules/cell, mean +/- SEM, n = 3). The ratio of the amounts of LTC4 and 15-lipoxygenase products was found to be strongly dependent on the arachidonic acid concentration, being relatively large at low arachidonic acid concentrations and very small at high arachidonic acid concentrations. Platelet activating factor (1 microM) was able to enhance significantly the production of LTC4 but not that of 15-lipoxygenase products. As arachidonic acid was found to be capable of inducing a fast, transient rise in the cytosolic free Ca2+ concentration, this explains, at least partly, its ability to induce the Ca2+-dependent formation of LTC4.
Kok
P T
PT
Department of Pulmonary Disease, State University of Utrecht, The Netherlands.
Hamelink
M L
ML
Kijne
G M
GM
Verhagen
J
J
Koenderman
L
L
Veldink
G A
GA
Bruynzeel
P L
PL
D001095:Arachidonic Acids / Q000494:pharmacology*; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004804:Eosinophils / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D013189:SRS-A / Q000096:biosynthesis*
D001095:Arachidonic Acids; D013189:SRS-A; D000001:Calcimycin; D002118:Calcium