[The rapid identification of E. coli by the detection of 3 enzymes].
232 isolates of gram-negative, oxidase-negative bacteria, isolated from various samples of different animal species, were tested with help of RAPIDEC coli for the production of beta-glucuronidase, beta-galactosidase and indole. The test correctly identified 164 of 175 E. coli strains (sensitivity 93.7%) and correctly indicated that 57 of 57 isolates of the family Enterobacteriaceae (7 Citrobacter sp., 18 Enterobacter sp., 16 Klebsiella sp., 10 Proteus sp., 2 Providencia sp. and 4 Salmonella sp.) were not E. coli (specificity 100%).
Weber
A
A
Schäfer
R
R
D000818:Animals; D004926:Escherichia coli / Q000201:enzymology / Q000302:isolation & purification*; D005696:Galactosidases / Q000032:analysis*; D005966:Glucuronidase / Q000032:analysis*; D008190:Lyases / Q000032:analysis*; D011237:Predictive Value of Tests; D013997:Time Factors; D014368:Tryptophanase / Q000032:analysis*; D001616:beta-Galactosidase / Q000032:analysis*
D005696:Galactosidases; D001616:beta-Galactosidase; D005966:Glucuronidase; D008190:Lyases; D014368:Tryptophanase
Zur schnellen Identifizierung von E. coli durch den Nachweis von 3 Enzymen.