title: Ultrastructure and complex carbohydrate ultracytochemistry of the cat parotid gland.
abstract: The structure and glycoconjugate content of the cat parotid gland were analyzed at electron microscopic level by applying morphological techniques and three ultrastructural histochemical methods - HID-TCH-SP, LID-TCH-SP and PA-TCH-SP. This gland appeared as a typical salivary gland composed of acinar secretory cells, intercalated ducts, striated ducts and excretory ducts. The most common configuration of secretory granules consisted of a dense core surrounded by a variable electron-lucent halo. All ductal segments were characterized by the presence of different cell populations and small apical granules greatly different from those localized in the acinar cells. By using HID-TCH-SP we were able to demonstrate that in a few acinar cells there are sulphated sites, whereas PA-TCH-SP staining revealed the presence of vic-glycol radicals in all acinar cells preferentially located on the halo of secretory granules.
authors.affiliation: Dipartimento di Biologia Cellulare, Università di Camerino, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Menghi
authors.forename: A M
authors.initials: AM
authors.lastname: Bondi
authors.forename: D
authors.initials: D
authors.lastname: Accili
authors.forename: L
authors.initials: L
authors.lastname: Fumagalli
mesh_terms: D000818:Animals; D002241:Carbohydrates / Q000032:analysis*; D002415:Cats; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D005260:Female; D010306:Parotid Gland / Q000737:chemistry / Q000648:ultrastructure*; D013045:Species Specificity
chemical_list: D002241:Carbohydrates