title: Characterization and functional expression in mammalian cells of genomic and cDNA clones encoding a Drosophila muscarinic acetylcholine receptor.
abstract: Genomic and cDNA clones encoding a muscarinic acetylcholine receptor from Drosophila melanogaster have been isolated. Sequence analysis demonstrates that this gene encodes a receptor with a high degree of amino acid identity to the mammalian muscarinic acetylcholine receptors and has three introns in the portion of the gene encoding the third putative cytoplasmic loop. A full-length cDNA clone has been placed under the control of the mouse metallothionein promotor and transfected into mouse Y1 adrenal cells. The receptor expressed in these cells exhibits the high-affinity binding for the antagonists quinuclidinyl benzilate and atropine expected of a muscarinic receptor. The Drosophila muscarinic receptor, when expressed in Y1 cells, is physiologically active, as measured by agonist-dependent stimulation of phosphatidylinositol metabolism.
authors.affiliation: Department of Pharmacology, University of Washington, Seattle 98195.
authors.forename: R A
authors.initials: RA
authors.lastname: Shapiro
authors.forename: B T
authors.initials: BT
authors.lastname: Wakimoto
authors.forename: E M
authors.initials: EM
authors.lastname: Subers
authors.forename: N M
authors.initials: NM
authors.lastname: Nathanson
mesh_terms: D000311:Adrenal Glands; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004331:Drosophila melanogaster / Q000235:genetics*; D015870:Gene Expression; D015723:Gene Library; D005796:Genes; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011976:Receptors, Muscarinic / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
chemical_list: D011976:Receptors, Muscarinic; D004247:DNA