Digital subtraction chromatography: an LC-technique to reduce disturbing peaks in bioanalysis.
A method of reducing disturbing peaks in LC-chromatograms is described. The technique, here called digital subtraction chromatography (DSCh), makes use of the new generation of integrating chromatographic data processors. These processors are able to store the LC-detector signal and then subtract one chromatogram from another with the resulting chromatogram reintegrated and reprinted. The possibility of getting a useful chromatogram by subtracting a zero sample chromatogram from a sample with disturbing peaks is demonstrated. The theoretical background of the method is given together with a discussion of the optimisation of the chromatographic conditions. A bioanalytical application is given.
Wiese
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Swedish University of Agricultural Sciences, Faculty of Veterinary Medicine, Department of Pharmacology and Toxicology, Uppsala Biomedical Centre.
D002701:Chloramphenicol / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods*; D003023:Cloxacillin / Q000032:analysis; D005260:Female; D006801:Humans; D008895:Milk, Human / Q000737:chemistry; D010400:Penicillin G / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet; D013382:Subtraction Technique
D002701:Chloramphenicol; D003023:Cloxacillin; D010400:Penicillin G