Complement C3 proteins in psoriasis.
A new polymorphism of the complement factor C3 in human plasma was demonstrated by isotachophoresis in agarose gels followed by immunodetection with rabbit anti-human C3c and C3d immunoglobulins. Four bands were detected in the immunoprint of freshly drawn EDTA-plasma, which were C3s1, C3s2, C3f1 and C3f2. At least four additional C3 components in Mg2+ -zymosan activated plasma were present, which were C3b1 to C3b4. The different forms of C3 in frozen and thawed heparin-plasma from 20 patients with psoriasis and 20 healthy individuals were studied from the immunoprint. The total content of C3 components was 29% greater in the patients with psoriasis than controls. The major difference was in the C3b components which were increased by 46%. In psoriatic patients, the two slow C3 components C3s1 and C3s2 were increased by 24 and 56% respectively, when compared with controls. The two fast C3 components C3f1 and C3f2 were decreased to 29 and 37%. The results suggest a direct involvement of the complement factor C3 in psoriasis.
Acevedo
F
F
Department of Dermatology, Karolinska Sjukhuset, Karolinska Institute, Stockholm, Sweden.
Hammar
H
H
D000328:Adult; D000368:Aged; D003167:Complement Activation; D003176:Complement C3 / Q000032:analysis* / Q000145:classification; D004492:Edetic Acid; D004587:Electrophoresis, Agar Gel; D006801:Humans; D015151:Immunoblotting; D008875:Middle Aged; D011565:Psoriasis / Q000276:immunology*
D003176:Complement C3; D004492:Edetic Acid