Chromosome banding analysis by slit-scan flow cytometry.
We have investigated the use of fluorescence banding patterns for the resolution of metaphase chromosomes by slit-scan flow cytometry. Fluorescence scans of R-banded chromosomes have been obtained for the entire human karyotype. Metaphase chromosomes were R-banded in suspension by staining with chromomycin A3 after hypotonic treatment in Ohnuki's buffer. Specific fluorescent landmark bands were detected for human chromosomes 1-12. Scans obtained for chromosomes 13-22 did not contain sufficient information for classification. Characteristic fluorescence patterns for human chromosomes 1 and 3 provided the clearest evidence for the detection of R-bands by slit-scan flow cytometry. Specific patterns were detected for human chromosomes 9-12 in which the number and placement of the fluorescent bands served as classifiers.
Bartholdi
M F
MF
Life Sciences Division, Los Alamos National Laboratory, New Mexico 87545.
Meyne
J
J
Johnston
R G
RG
Cram
L S
LS
D002460:Cell Line; D014128:Chromomycin A3; D002871:Chromosome Banding / Q000379:methods*; D002877:Chromosomes, Human / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007621:Karyotyping / Q000379:methods*
D014128:Chromomycin A3