Follow up of T cells subsets and anti-trisaccharide IgM antibody levels of leprosy patients during daily multidrug therapy.
T cell subset distribution and anti-M. leprae specific antibody levels were followed in leprosy patients during polychemotherapy (or multidrug therapy, MDT). Before treatment, the T cell subset counts showed that the multibacillary patients (28) have a significant decrease (p less than 0.05) in the CD3+, CD4+ T percentages and CD4+/CD8+ ratio as compared to the healthy controls (96). The paucibacillary patients (30) had no significant T cell disturbances. An ELISA test has been developed using the natural trisaccharide-ocytl-BSA (NTO) antigen to identify specific IgM antibodies (IgM anti-T). Multibacillary patients (22) showed higher antibody titers than paucibacillary patients (11). But the IgM anti-T levels of the two groups are significantly higher than controls belonging to the same ethnic group. A daily polychemotherapy (Dapsone, Rifampicine, Clofazimine) was done for 6 months in paucibacillary patients and 2 years in multibacillary patients according to the leprosy group study recommendations (Prof. Grosset, Hôptical de la Salpêtrière, Paris, France). A normalization of the CD4+ T cells was obtained by a 6 month therapy in multibacillary patients and a significant decrease in the IgM anti-levels was obtained during polychemotherapy in multibacillaries (p less than 0.001) and paucibacillaries (p less than 0.05).
Desforges
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S
Pasteur Institute, Noumea, New Caledonia.
Launois
P
P
Bobin
P
P
Bach
M A
MA
D000907:Antibodies, Bacterial / Q000032:analysis*; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis; D007917:Leprostatic Agents / Q000627:therapeutic use; D007918:Leprosy / Q000097:blood / Q000188:drug therapy / Q000276:immunology*; D007958:Leukocyte Count; D009166:Mycobacterium leprae / Q000276:immunology; D013601:T-Lymphocytes / Q000145:classification* / Q000276:immunology; D014312:Trisaccharides / Q000276:immunology
D000907:Antibodies, Bacterial; D007075:Immunoglobulin M; D007917:Leprostatic Agents; D014312:Trisaccharides