Identification of polysaccharides in pharmaceuticals by capillary gas chromatography.
A sensitive method for the identification of polysaccharides in pharmaceuticals is described. Polysaccharides are isolated by gel filtration and subsequently hydrolysed. The monomeric carbohydrates obtained are transformed into oxime-trimethylsilyl derivatives and analysed by capillary gas chromatography. Profiles of 13 different natural or semi-synthetic polysaccharides are discussed. The profiles of the hydrolysis products can be used to identify the polysaccharides mentioned above. Possible interferences by other polymers are given. The method can be used to identify most polysaccharides used as pharmaceutical adjuvants.
Vanlaeke
G
G
Dr. L. Willems-Instituut, Universitaire campus, Diepenbeek, Belgium.
Cuppens
H
H
Leyssens
L
L
Raus
J
J
D000438:Alcohols / Q000032:analysis; D000688:Amylose / Q000031:analogs & derivatives / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D002266:Carboxymethylcellulose Sodium / Q000032:analysis; D002482:Cellulose / Q000031:analogs & derivatives / Q000032:analysis; D002849:Chromatography, Gas; D002850:Chromatography, Gel; D004655:Emulsions; D006868:Hydrolysis; D007202:Indicators and Reagents; D009824:Ointments; D011134:Polysaccharides / Q000032:analysis*; D013464:Sulfuric Acids / Q000032:analysis; D014297:Trimethylsilyl Compounds / Q000032:analysis / Q000737:chemistry; D014574:Uronic Acids / Q000032:analysis
D000438:Alcohols; D002241:Carbohydrates; D004655:Emulsions; D007202:Indicators and Reagents; D009824:Ointments; D011134:Polysaccharides; D013464:Sulfuric Acids; D014297:Trimethylsilyl Compounds; D014574:Uronic Acids; C027357:carboxymethylamylose; C013517:ethyl cellulose; D002482:Cellulose; D000688:Amylose; D002266:Carboxymethylcellulose Sodium; C033158:sulfuric acid