title: Enzyme reactions involved in anaerobic cyclohexanol metabolism by a denitrifying Pseudomonas species.
abstract: The enzymes involved in the anaerobic degradation of cyclohexanol were searched for in a denitrifying Pseudomonas species which metabolizes this alicyclic compound to CO2 anaerobically. All postulated enzyme activities were demonstrated in vitro with sufficient specific activities. Cyclohexanol dehydrogenase catalyzes the oxidation of the substrate to cyclohexanone. Cyclohexanone dehydrogenase oxidizes cyclohexanone to 2-cyclohexenone. 2-Cyclohexenone hydratase and 3-hydroxycyclohexanone dehydrogenase convert 2-cyclohexenone via 3-hydroxycyclohexanone into 1,3-cyclohexanedione. Finally, the dione is cleaved by 1,3-cyclohexanedione hydrolase into 5-oxocaproic acid. Some kinetic and regulatory properties of these enzymes were studied.
authors.affiliation: Angewandte Mikrobiologie, Universität Ulm, Federal Republic of ffrmany.
authors.forename: W
authors.initials: W
authors.lastname: Dangel
authors.forename: A
authors.initials: A
authors.lastname: Tschech
authors.forename: G
authors.initials: G
authors.lastname: Fuchs
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D002245:Carbon Dioxide / Q000378:metabolism; D003511:Cyclohexanols / Q000378:metabolism*; D007700:Kinetics; D011549:Pseudomonas / Q000254:growth & development / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D003511:Cyclohexanols; D002245:Carbon Dioxide