title: Detection of calcitonin and CGRP mRNAs in thyroid parafollicular cells using biotinylated probes.
abstract: Probes for hybridocytochemistry were produced using SP6 RNA polymerase system and biotinylated 11-UTP as a label. Probes were obtained capable of detecting calcitonin-mRNA and CGRP-mRNA. Probe quality was tested using Southern blotting. Hybridocytochemistry on rat thyroid sections demonstrated both mRNAs in all parafollicular cells.
authors.affiliation: Department of Histology, Medical Academy, Poznań, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Zabel
authors.forename: H
authors.initials: H
authors.lastname: Schäfer
mesh_terms: D000818:Animals; D002116:Calcitonin / Q000235:genetics*; D015740:Calcitonin Gene-Related Peptide; D005786:Gene Expression Regulation; D009479:Neuropeptides / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D015347:RNA Probes; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D013961:Thyroid Gland / Q000033:anatomy & histology*
chemical_list: D009479:Neuropeptides; D015347:RNA Probes; D012333:RNA, Messenger; D002116:Calcitonin; D015740:Calcitonin Gene-Related Peptide