Colorimetric methods for the assay of nomifensine maleate.
Two colorimetric methods are reported for the assay of nomifensine maleate. The methods are based on coupling between the diazotised form of nomifensine maleate and (i) N-(1-naphthyl)-ethylene diamine dihydrochloride (Bratton-Marshall reagent) and (ii) p-aminosalicylic acid (PAS). The optimum conditions for the reactions were investigated. The coupled products exhibit maximum absorbance at 470 and 435 nm for the Bratton-Marshall and PAS reagents, respectively. With PAS, a linear relationship has been established between absorbance (Amax) and concentration of nomefensine maleate over the range 2-12 micrograms ml-1. Similarly, with the Bratton-Marshall reagent, a linear relationship exists in the concentration range 2-16 micrograms ml-1. The calculated mean percent recoveries for nomifensine maleate in the commercial capsules (Merital 25 mg) respectively. Similarly, for the added recoveries, the percentage obtained were 99.01 +/- 0.46 and 100.03 +/- 1.03, respectively.
Abounassif
M A
MA
Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
Mohamed
M E
ME
Aboul-Enein
H Y
HY
D010131:Aminosalicylic Acid; D003124:Colorimetry; D007202:Indicators and Reagents; D009627:Nomifensine / Q000032:analysis* / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
D007202:Indicators and Reagents; D009627:Nomifensine; D010131:Aminosalicylic Acid