Enhancement of Aspergillus nidulans transformation by the ans1 sequence.
We have shown that the Aspergillus nidulans ans1 sequence enhances the efficiency of transformation when introduced into vectors containing argB or trpC genes. Increased efficiency of transformation is also observed when ans1 is present on a second cotransforming plasmid. In an attempt to explains the ans1 transactivity we have performed analysis of some cotransformants.
Dzikowska
A
A
Department of Genetics, University of Warsaw, Poland.
Wegleński
P
P
D001233:Aspergillus nidulans / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010957:Plasmids / Q000235:genetics; D014169:Transformation, Bacterial / Q000235:genetics*
D004269:DNA, Bacterial