title: Utilization of D-beta-hydroxybutyrate and oleate as alternate energy fuels in brain cell cultures of newborn mice after hypoxia at different glucose concentrations.
abstract: In dissociated whole brain cell cultures from newborn mice, we have previously shown that during glucose deprivation under normoxia, D-beta-hydroxybutyrate and oleic acid are increasingly used for energy production. We now asked whether this glucose dependency of the utilization of D-beta-hydroxybutyrate and oleic acid as alternate energy fuels is also present after a hypoxic phase. 3-Hydroxy[3-14C]butyrate or [U-14C]oleic acid were added to 7- and 14-d-old cultures and 14CO2-production compared after hypoxia in normal and glucose-deprived conditions. After hypoxia, the ability of the cells 7 d in culture to increase D-beta-hydroxybutyrate consumption in response to glucose deprivation is diminished, 14-d-old cells lose this ability. In contrast, after hypoxia, both 7- and 14-d-old cultures maintain or even improve the ability to increase oleate consumption, when glucose is lacking.
authors.affiliation: Department of Pediatrics, University of Berne, Switzerland.
authors.forename: E
authors.initials: E
authors.lastname: Bossi
authors.forename: E
authors.initials: E
authors.lastname: Kohler
authors.forename: N
authors.initials: N
authors.lastname: Herschkowitz
mesh_terms: D020155:3-Hydroxybutyric Acid; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D002478:Cells, Cultured; D004734:Energy Metabolism; D005947:Glucose / Q000378:metabolism; D006885:Hydroxybutyrates / Q000378:metabolism*; D000860:Hypoxia / Q000378:metabolism; D051379:Mice; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism*
chemical_list: D006885:Hydroxybutyrates; D009829:Oleic Acids; D002245:Carbon Dioxide; D019301:Oleic Acid; D005947:Glucose; D020155:3-Hydroxybutyric Acid