Phosphorylation of glutaminyl-tRNA synthetase and threonyl-tRNA synthetase by the gene products of dnaK and dnaJ in Escherichia coli K-12 cells.
In Escherichia coli K-12, the heat shock protein DnaK and DnaJ participate in phosphorylation of both glutaminyl-tRNA synthetase and threonyl-tRNA synthetase since when cellular proteins extracted from the dnaK7(Ts), dnaK756(Ts) and dnaJ259(Ts) mutant cells labeled with 32Pi at 42 degrees C were analyzed by two-dimensional gel electrophoresis, no phosphorylation of glutaminyl-tRNA synthetase and threonyl-tRNA synthetase was observed while phosphorylation of both aminoacyl-tRNA synthetases was detected in the samples extracted from wild-type cells.
Itikawa
H
H
Department of Biology, Faculty of Science, Tokyo Metropolitan University, Japan.
Wada
M
M
Sekine
K
K
Fujita
H
H
D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005975:Glutamate-tRNA Ligase / Q000235:genetics / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000235:genetics*; D010766:Phosphorylation; D013914:Threonine-tRNA Ligase / Q000235:genetics / Q000378:metabolism*
D006360:Heat-Shock Proteins; D000604:Amino Acyl-tRNA Synthetases; D005975:Glutamate-tRNA Ligase; D013914:Threonine-tRNA Ligase