title: Trifluorothymidine localization in the rabbit cornea by secondary ion mass spectrometry imaging microanalysis.
abstract: Imaging microanalysis by secondary ion mass spectrometry is a sensitive surface analytical technique that allows the detection and localization of elements and compounds in biological tissues. We report the detection by this approach of intracorneal trifluorothymidine following topical administration in rabbit with normal cornea. The presence of trifluorothymidine is revealed using 19F as a marker, allowing the acquisition of ultrastructural microanalytical images without using radioactive tracers.
authors.affiliation: Laboratoire d'Ophtalmologie, Hôtel-Dieu, Paris, France.
authors.forename: J M
authors.initials: JM
authors.lastname: Legeais
authors.forename: P
authors.initials: P
authors.lastname: Hallegot
authors.forename: J
authors.initials: J
authors.lastname: Chabala
authors.forename: G
authors.initials: G
authors.lastname: Renard
authors.forename: R
authors.initials: R
authors.lastname: Levi-Setti
authors.forename: P
authors.initials: P
authors.lastname: Galle
mesh_terms: D000818:Animals; D003315:Cornea / Q000032:analysis*; D007498:Iris / Q000032:analysis; D013058:Mass Spectrometry / Q000379:methods; D011817:Rabbits; D013936:Thymidine; D014271:Trifluridine / Q000032:analysis* / Q000493:pharmacokinetics
chemical_list: D014271:Trifluridine; D013936:Thymidine