Enzymatic hydrolysis of 7-phenylacetamidodesacetoxycephalosporanic acid by immobilized penicillin G acylase.
Enzymatic parameters such as pH, temperature and substrate concentration were studied for the hydrolysis of 7-PADCA by penicillin G acylase. Optimum pH and temperature were 8.0 and 50 degrees C, respectively. Km value of soluble and immobilized enzyme for 7-PADCA was 2.3 x 10(-5) M and 7.5 x 10(-5) M, respectively. At 7-PADCA concentration of 5% and an IME: 7-PADCA ratio of 1:2.5, the hydrolysis was complete in 110 min.
Sudhakaran
V K
VK
Deshpande
B S
BS
Ambedkar
S S
SS
Shewale
J G
JG
D002511:Cephalosporins / Q000378:metabolism*; D004800:Enzymes, Immobilized; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D010399:Penicillin Amidase / Q000378:metabolism*; D013696:Temperature
D002511:Cephalosporins; D004800:Enzymes, Immobilized; C013644:7-phenylacetamidodeacetoxycephalosporanic acid; D010399:Penicillin Amidase