title: Chicken IgL gene rearrangement involves deletion of a circular episome and addition of single nonrandom nucleotides to both coding segments.
abstract: Chicken immunoglobulin light chain (IgL) gene rearrangement has been characterized. Rearrangement of the single variable (VL) segment with the single joining (JL) segment within the chicken IgL locus results in the deletion of the DNA between VL and JL from the genome. This deletion is accomplished by a molecular mechanism in which a precise joining of the IgL recombination signal sequences leads to the formation of a circular episomal element. The circular episome is an unstable genetic element that fails to be propagated during B cell development. Evidence was obtained that the formation of the circular episome is accompanied by the addition of a single nonrandom base to both the VL and JL coding segments. The subsequent joining of the VL and JL segments appears to occur at random, as we observed at least 25 unique V-J junction sequences, 11 of which are out-of-frame. A novel recombination mechanism that accounts for the observed features of chicken IgL gene rearrangement is discussed.
authors.affiliation: Howard Hughes Medical Institute, University of Michigan Medical School, Ann Arbor 48109.
authors.forename: W T
authors.initials: WT
authors.lastname: McCormack
authors.forename: L W
authors.initials: LW
authors.lastname: Tjoelker
authors.forename: L M
authors.initials: LM
authors.lastname: Carlson
authors.forename: B
authors.initials: B
authors.lastname: Petryniak
authors.forename: C F
authors.initials: CF
authors.lastname: Barth
authors.forename: E H
authors.initials: EH
authors.lastname: Humphries
authors.forename: C B
authors.initials: CB
authors.lastname: Thompson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D002872:Chromosome Deletion; D015328:Gene Rearrangement, B-Lymphocyte, Light Chain; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D013329:Structure-Activity Relationship
chemical_list: D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region