Anti-interferon-gamma antibodies in sera from HIV infected patients.
High serum levels of antibodies to interferon-gamma (IFN-gamma) have been found in patients infected with human immunodeficiency virus (HIV). A radioimmunoassay (RIA) with a recombinant IFN-gamma protein or an affinity purified IFN-gamma preparation as antigens, was developed to detect the specific anti-IFN-gamma antibodies. Reactivity of sera to IFN-gamma was confirmed by Western blot analysis. These antibodies, however, do not seem to recognize the active site of the molecule, since they do not neutralize the antiviral IFN-gamma activity in a biological assay. These results enforce the hypothesis of the role of autoimmunization during the course of the disease.
Caruso
A
A
Institute of Microbiology, University of Brescia, Italy.
Foresti
I
I
Gribaudo
G
G
Bonfanti
C
C
Pollara
P
P
Dolei
A
A
Landolfo
S
S
Turano
A
A
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology*; D000328:Adult; D001323:Autoantibodies / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology; D015153:Blotting, Western; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007371:Interferon-gamma / Q000276:immunology*; D011863:Radioimmunoassay
D001323:Autoantibodies; D007074:Immunoglobulin G; D007371:Interferon-gamma