Expression in Bacillus subtilis of the 51- and 42-kilodalton mosquitocidal toxin genes of Bacillus sphaericus.
A 3,080-base-pair KpnI-HindIII DNA fragment from Bacillus sphaericus 2362 coding for 51- and 42-kilodalton mosquitocidal proteins was cloned into Bacillus subtilis DB104 by using the vector pUB18. In B. subtilis these proteins were not detected during vegetative growth but were expressed during sporulation at levels comparable to those found in B. sphaericus.
Baumann
L
L
Department of Bacteriology, University of California, Davis 95616.
Baumann
P
P
D001407:Bacillus / Q000235:genetics* / Q000502:physiology; D001412:Bacillus subtilis / Q000235:genetics* / Q000502:physiology; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D001427:Bacterial Toxins / Q000235:genetics*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D005786:Gene Expression Regulation; D015151:Immunoblotting; D015183:Restriction Mapping; D013171:Spores, Bacterial
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004269:DNA, Bacterial; C046134:binB protein, Bacillus sphaericus