Recombinant gene expression in vivo within endothelial cells of the arterial wall.
A technique for the transfer of endothelial cells and expression of recombinant genes in vivo could allow the introduction of proteins of therapeutic value in the management of cardiovascular diseases. Porcine endothelial cells expressing recombinant beta-galactosidase from a murine amphotropic retroviral vector were introduced with a catheter into denuded iliofemoral arteries of syngeneic animals. Arterial segments explanted 2 to 4 weeks later contained endothelial cells expressing beta-galactosidase, an indication that they were successfully implanted on the vessel wall.
Nabel
E G
EG
Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor 48109-0650.
Plautz
G
G
Boyce
F M
FM
Stanley
J C
JC
Nabel
G J
GJ
D000818:Animals; D002406:Catheterization, Peripheral; D004274:DNA, Recombinant; D004730:Endothelium, Vascular / Q000166:cytology* / Q000201:enzymology / Q000637:transplantation; D005260:Female; D005696:Galactosidases / Q000096:biosynthesis*; D005822:Genetic Vectors; D007083:Iliac Artery / Q000166:cytology; D012190:Retroviridae; D013552:Swine; D013556:Swine, Miniature; D001616:beta-Galactosidase / Q000096:biosynthesis*
D004274:DNA, Recombinant; D005696:Galactosidases; D001616:beta-Galactosidase