Test of ML23 as an antagonist to the effects of melatonin in the ram.
In Exp. 1, four groups of 8 yearling Soay rams were housed under long days (16L:8D) to induce reproductive quiescence and were treated daily for 12 weeks with: (I) vehicle (2 or 4 ml 50% ethanol/water), (II) ML23 (2 mg), (III) melatonin (2 mg) and (IV) melatonin and ML23 (2 mg of each). All treatments were given orally in the mid-light phase. In the rams receiving melatonin (Group III) there was an earlier increase in the plasma concentrations of FSH and testosterone and regrowth of the testes compared to the controls (time to maximum testicular diameter: 10.0 +/- 0.5 and 15.3 +/- 1.2 weeks). These differences were reversed after the end of the 12-week treatments when rapid testicular regression occurred in melatonin-treated rams but not in the controls. In the group receiving ML23 and melatonin (Group IV), there was early reactivation and regression of the reproductive axis as in the melatonin group (testis max. 9.9 +/- 0.7 and 10.0 +/- 0.5 weeks) while in the group receiving ML23 alone (Group II) there was a slower redevelopment and regression as in the controls (testis max. 15.7 +/- 1.1 and 15.3 +/- 1.2 weeks). The comparison between the 4 groups in the changes in the blood concentrations of prolactin, voluntary food intake and total body weight also indicated that the treatment with ML23 failed to modify the effect of melatonin (combined treatment vs melatonin) or the effect of the long day photoperiod (ML23 vs vehicle).(ABSTRACT TRUNCATED AT 250 WORDS)
Lincoln
G A
GA
MRC Reproductive Biology Unit, Edinburgh, UK.
Kelly
R W
RW
D008735:5-Methoxytryptamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D005640:Follicle Stimulating Hormone / Q000097:blood; D008027:Light; D008239:Lysine / Q000494:pharmacology; D008297:Male; D008550:Melatonin / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D012098:Reproduction / Q000187:drug effects*; D012701:Serotonin / Q000031:analogs & derivatives*; D013737:Testis / Q000033:anatomy & histology; D013739:Testosterone / Q000097:blood
C053008:ML 23; D012701:Serotonin; D008735:5-Methoxytryptamine; D013739:Testosterone; D005640:Follicle Stimulating Hormone; D008550:Melatonin; D008239:Lysine