Heterogeneity of the L-rhamnose residue in the outer core of Pseudomonas aeruginosa lipopolysaccharide, characterized by using human monoclonal antibodies.
Hybridoma cell lines producing human monoclonal antibodies (MAbs) MH-4H7 and KN-2B11 [immunoglobulin M (lambda)] which bound to the outer core region of Pseudomonas aeruginosa lipopolysaccharide (LPS) were established by cell fusion of human peripheral lymphocytes with human-mouse heteromyeloma SHM D-33. Both binding specificity experiments with a series of LPS-defective mutants derived from P. aeruginosa PAC1R (P. S. N. Rowe and P. M. Meadow, Eur. J. Biochem.132:329-337, 1983) and competitive enzyme immunoassay experiments with monosaccharides demonstrated that alpha-L-rhamnose residues in the outer core of LPS might be in part an epitope. The MAbs specifically bound to clinical isolates belonging to Homma serotypes A, F, G, and K at a frequency of 70 to 86% and to serotypes H and M isolates at about 50%. They did not bind to any isolates of serotype B, E, and I tested. This evidence indicates that L-rhamnose and probably its neighboring residues in the other core of P. aeruginosa are heterogeneous in some association with the O serotype.
Yokota
S
S
Laboratory of Biotechnology, Sumitomo Chemical Co., Ltd., Hyogo, Japan.
Ochi
H
H
Ohtsuka
H
H
Kato
M
M
Noguchi
H
H
D000328:Adult; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology; D001666:Binding Sites, Antibody; D015153:Blotting, Western; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002460:Cell Line; D006801:Humans; D006825:Hybridomas / Q000032:analysis; D008070:Lipopolysaccharides / Q000032:analysis* / Q000276:immunology; D008969:Molecular Sequence Data; D011550:Pseudomonas aeruginosa / Q000032:analysis* / Q000145:classification / Q000276:immunology; D012210:Rhamnose / Q000032:analysis* / Q000276:immunology; D012703:Serotyping
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D008070:Lipopolysaccharides; D012210:Rhamnose