Photoreceptor pigments for photomovement of microorganisms: some spectroscopic and related studies.
Optical spectroscopy of photoreceptor pigments can substantially contribute to our understanding of the molecular processes which are the basis of photoreception and sensory transduction in photomotile microorganisms. The main spectroscopic techniques are briefly illustrated, together with the most significant types of progress that can be achieved. A few "case examples" are discussed in some detail: Halobacterium, with particular attention to the contribution of flash photolysis studies to the identification and characterization of sensory rhodopsins; Euglena, and the role of in vivo microspectrofluorometry in confirming the flavin nature of its photoreceptor pigment; the first suggestions on the rhodopsin-like nature of the Chlamydomonas photosensing system; Stentor and Blepharisma and the contribution of static and time-resolved fluorescence studies to a molecular model of the primary events in their photoreceptor pigments (stentorin and blepharismin) and systems.
Lenci
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F
C.N.R. Istituto di Biofisica, Pisa, Italy.
Ghetti
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F
D000818:Animals; D001419:Bacteria / Q000528:radiation effects*; D056890:Eukaryota / Q000528:radiation effects*; D010786:Photoreceptor Cells; D010860:Pigments, Biological / Q000032:analysis*
D010860:Pigments, Biological