EDTA-polyvinylpyrrolidone as a preservation medium for immunohistochemistry.
In a solution on 7% EDTA and 5% polyvinylpyrrolidone in 0.1 mol/l. Tris buffer, pH = 6.95, tissues samples can be stored at room temperature for 1 week without detectable loss of structural integrity or antigenicity (as demonstrated in immunohistochemistry with monoclonal antibodies to intermediate filament proteins).
Stosiek
P
P
District Hospital, Görlitz.
Kasper
M
M
Karsten
U
U
D014408:Biomarkers, Tumor / Q000032:analysis; D004492:Edetic Acid; D006801:Humans; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000032:analysis; D009369:Neoplasms / Q000032:analysis; D011205:Povidone; D012996:Solutions; D014021:Tissue Preservation
D014408:Biomarkers, Tumor; D007381:Intermediate Filament Proteins; D012996:Solutions; D004492:Edetic Acid; D011205:Povidone