Serological classification in relation to auxotypes, plasmid contents, and susceptibilities to antimicrobials of PPNG and non-PPNG strains isolated in Greece.
Thirty three penicillinase producing Neisseria gonorrhoeae (PPNG) and 152 non-PPNG strains were serologically classified in relation to their patterns of resistance to antimicrobials, nutritional requirements (auxotypes), and plasmid contents. Of the 185 strains, 65 (35%) belonged to the WI and 120 (65%) to the WII/III serogroup, the predominant serovars of which were Arost and Bropt, respectively. Associations between serotype and susceptibility to antimicrobial agents, auxotype, and plasmid content were observed. Of the 152 non-PPNG strains, 112 (74%) belonged to serogroup WI. The WI non-PPNG strains were more sensitive to penicillin, thiamphenicol, tetracycline, and cefotaxime than the WII/III non-PPNG strains. Auxotyping and serogrouping the strains showed no differentiation other than that arginine, hypoxanthine, and uracil dependent (AHU-) strains belonged to serogroup WI. The proline, citrulline, and uracil dependent (PCU-) strains belonged, as expected, to serogroup WII/III. Analysing the 33 PPNG strains showed that of 21 carrying the 4.5 megadalton (Asian type) penicillin resistance plasmid, 17 (81%) belonged to serogroup WI, with serovar Arst predominating, and only four (19%) to serogroup WII/III. Of 11 isolates carrying the 3.2 megadalton (African type) resistance plasmid, seven (64%) belonged to serogroup WI (serovar Av predominating) and four (36%) to WII/III (with serovar Bropyst predominating). One strain carried the 3.05 megadalton penicillin resistance plasmid (Toronto type), belonged to the WI serogroup, and had serovar Arost.
Tzanakaki
G
G
Bacteriology Department, Hellenic Pasteur Institute, Athens, Greece.
Mavrommati
L
L
Tzelepi
E
E
Kolyva
S
S
Fragouli
E
E
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D006115:Greece; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D009344:Neisseria gonorrhoeae / Q000145:classification* / Q000187:drug effects / Q000201:enzymology; D010405:Penicillinase / Q000378:metabolism; D010957:Plasmids; D012703:Serotyping; D014526:Urethritis / Q000382:microbiology
D000900:Anti-Bacterial Agents; D010405:Penicillinase