Ultrastructural aspects of fragility of Pseudomonas aeruginosa outer membrane devoid of protein F.
The protein F-deficient cells of Pseudomonas aeruginosa were previously found to be more susceptible to osmotic shock than the sufficient cells (Gotoh et al., J. Bacteriol., in press). The protein F-deficient cells were observed by the thin-section method of electron microscopy to determine the effects of osmotic shock. The osmotic shock induced breakage of the protein F-deficient outer membrane, while it had no effect on the protein F-sufficient outer membrane. These results suggested that the cells lost their viability by the osmotic shock caused by fragility of the outer membrane.
Gotoh
N
N
Department of Microbiology, Kyoto Pharmaceutical University, Japan.
Wakebe
H
H
Nishino
T
T
D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D008854:Microscopy, Electron; D009997:Osmotic Pressure; D011550:Pseudomonas aeruginosa / Q000032:analysis*
D001425:Bacterial Outer Membrane Proteins