Identification of tumor promoters by their inhibitory effect on intercellular transfer of lucifer yellow.
The effect of the tumor promoters 12-O-tetradecanoylphorbol-13-acetate (TPA), mezerein, teleocidin, anthralin, the Ca2+-ionophore A23187, butylated hydroxytoluene (BHT), dichlorodiphenyltrichloroethane (DDT) and phenobarbital (PB) on lucifer yellow transfer in cultures of SV-40-transformed Djungarian hamster fibroblasts was studied. TPA, mezerein, teleocidin, A23187, DDT and BHT exerted a strong inhibitory effect on cell-to-cell dye transfer. Anthralin uncoupled cells in 3 experiments out of 6. PB appeared to enhance lucifer yellow transfer. Sodium nitrite, a substance with unknown promoting activity, effectively uncoupled cells. All the promoters investigated had a reversible effect on the dye transfer. The value of the dye transfer method for promoter screening is discussed.
Budunova
I V
IV
Laboratory of Carcinogen Screening Methods, All-Union Cancer Research Center of the AMS USSR, Moscow.
Mittelman
L A
LA
Belitsky
G A
GA
D000818:Animals; D000875:Anthralin / Q000032:analysis / Q000633:toxicity; D002084:Butylated Hydroxytoluene / Q000032:analysis / Q000633:toxicity; D000001:Calcimycin / Q000032:analysis / Q000633:toxicity; D002273:Carcinogens / Q000032:analysis / Q000633:toxicity*; D002478:Cells, Cultured; D006224:Cricetinae; D003634:DDT / Q000032:analysis / Q000633:toxicity; D004224:Diterpenes; D005456:Fluorescent Dyes; D007365:Intercellular Junctions / Q000187:drug effects*; D007546:Isoquinolines; D008235:Lyngbya Toxins / Q000032:analysis / Q000633:toxicity; D010634:Phenobarbital / Q000032:analysis / Q000633:toxicity; D013729:Terpenes / Q000032:analysis / Q000633:toxicity; D013755:Tetradecanoylphorbol Acetate / Q000032:analysis / Q000633:toxicity
D002273:Carcinogens; D004224:Diterpenes; D005456:Fluorescent Dyes; D007546:Isoquinolines; D008235:Lyngbya Toxins; D013729:Terpenes; D002084:Butylated Hydroxytoluene; C022176:teleocidins; C011309:mezerein; D000001:Calcimycin; C017475:lucifer yellow; D003634:DDT; D013755:Tetradecanoylphorbol Acetate; D000875:Anthralin; D010634:Phenobarbital