Acetaldehyde activation of poly(ADP-ribose)polymerase in hepatocytes of mice treated in vivo.
The activities of poly(ADP-ribose) polymerase and of DNA polymerases alpha and beta and the level of cytochrome P450 were determined in mouse parenchymal liver cells 5 h after treatment with 0.1, 0.3, 1.0, and 3.0 mumole of acetaldehyde. Injection with 1.0 and 3.0 mumole of acetaldehyde induced an increase in poly(ADP-ribose) polymerase activity and in the P450 level, but had no effect on DNA polymerases. The stimulation of poly(ADP-ribose) polymerase activity can be used as an index of induced DNA damage. The possibility of using this experimental approach with other cells derived from mice treated in vivo with different xenobiotics is discussed.
Clerici
L
L
Commission of the European Communities, Joint Research Centre, Ispra, Italy.
Sacco
M G
MG
Merlini
M
M
D000079:Acetaldehyde / Q000494:pharmacology*; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004249:DNA Damage; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D051379:Mice; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*
D003577:Cytochrome P-450 Enzyme System; D008741:Methyl Methanesulfonate; D011065:Poly(ADP-ribose) Polymerases; D004259:DNA-Directed DNA Polymerase; D000079:Acetaldehyde