Protective effect of lipoproteins containing apoprotein A-I on Cu2+-catalyzed oxidation of human low density lipoprotein.
Two apoprotein A-I (apoA-I)-containing lipoproteins, one containing apoA-I and apoA-II (LpA-I/A-II) and the other containing only apoA-I (LpA-I), were examined for their effect on Cu2+-mediated oxidation of low density lipoprotein (LDL). The presence of LpA-I or LpA-I/A-II prevented LDL oxidation when assessed by the electrophoretic mobility, apoprotein B fragmentation and amounts of thiobarbituric acid-reactive substances. The protection of LDL oxidation by these lipoproteins was effective for up to 6 h, with LpA-I being more active than LpA-I/A-II. Results from these in vitro model experiments raise a possibility that LpA-I may play a role in protecting LDL from Cu2+-mediated oxidation.
Ohta
T
T
Department of Pediatrics, Kumamoto University Medical School, Japan.
Takata
K
K
Horiuchi
S
S
Morino
Y
Y
Matsuda
I
I
D016632:Apolipoprotein A-I; D001054:Apolipoproteins A / Q000378:metabolism*; D002413:Cations, Divalent; D003300:Copper / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D006801:Humans; D066298:In Vitro Techniques; D008075:Lipoproteins, HDL / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000378:metabolism*; D010084:Oxidation-Reduction
D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D002413:Cations, Divalent; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D003300:Copper