Partial purification of a macrophage-activating factor for glucose consumption (MAF-G) produced by a human T-cell hybridoma and its relation to a growth-promoting factor.
A material that enhances the glucose consumption of oil-induced peritoneal macrophages of guinea pig, the activity being named MAF-G activity, was purified approximately 780-fold from human T cell hybridoma conditioned medium. A material showing growth-promoting activity was co-purified with that showing the MAF-G activity from the same conditioned medium. The two materials showed an identical isoelectric point of pH 4.7. When the partially purified MAF-G increased glucose consumption, it did not induce glucose carbon-1 oxidation. MAF-G activity was inhibited by mitomycin C and colchicine, which inhibit DNA synthesis and mitosis, respectively, but not by 2-deoxy-D-glucose, an inhibitor of glucose metabolism. The partially purified MAF-G also enhanced the glucose consumption and proliferation of human monocytes. These observations suggest that MAF-G may be identical with the growth-promoting factor for macrophages.
Osada
H
H
Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Furukawa-Nakanishi
K
K
Miyamoto
D
D
Kobayashi
Y
Y
Osawa
T
T
D000818:Animals; D002850:Chromatography, Gel; D003078:Colchicine / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D006133:Growth Substances / Q000302:isolation & purification; D006168:Guinea Pigs; D006801:Humans; D006825:Hybridomas; D066298:In Vitro Techniques; D007526:Isoelectric Point; D008222:Lymphokines / Q000302:isolation & purification*; D008262:Macrophage Activation; D016215:Macrophage-Activating Factors; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D009000:Monocytes / Q000166:cytology / Q000378:metabolism; D013601:T-Lymphocytes / Q000502:physiology*
D006133:Growth Substances; D008222:Lymphokines; D016215:Macrophage-Activating Factors; D008937:Mitomycins; D016685:Mitomycin; D005947:Glucose; D003078:Colchicine