title: cis-regulatory elements of the Drosophila blastoderm-specific serendipity alpha gene: ectopic activation in the embryonic PNS promoted by the deletion of an upstream region.
abstract: The Drosophila serendipity alpha gene (sry alpha) is specifically expressed at the blastoderm stage in all somatic cells. By deletion analysis of sry alpha-lacZ fusion genes, the sry alpha cis-acting regulatory elements have been restricted to the [-311, +130] 5'-region of the gene and separated in two domains. The [-118, +130] domain is sufficient for transcriptional activation at the blastoderm stage. The [-311, -118] domain is required for a full level of expression. Deletion of this upstream domain leads to a secondary pattern of lacZ expression in precursor cells of the peripheral nervous system (PNS). The sry alpha gene is not itself secondarily expressed in the PNS, as shown by in situ hybridization. The patterns of expression of the different sry alpha-lacZ fusion genes suggest a combinatorial mode of regulation of sry alpha expression at blastoderm.
authors.affiliation: Institut Jacques Monod, CNRS, Paris, France.
authors.forename: F
authors.initials: F
authors.lastname: Schweisguth
authors.forename: C
authors.initials: C
authors.lastname: Yanicostas
authors.forename: F
authors.initials: F
authors.lastname: Payre
authors.forename: J A
authors.initials: JA
authors.lastname: Lepesant
authors.forename: A
authors.initials: A
authors.lastname: Vincent
mesh_terms: D000818:Animals; D001756:Blastoderm / Q000378:metabolism*; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics*; D005696:Galactosidases / Q000235:genetics; D005786:Gene Expression Regulation / Q000235:genetics*; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010525:Peripheral Nerves / Q000196:embryology; D015347:RNA Probes; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D014158:Transcription, Genetic / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D004274:DNA, Recombinant; D015347:RNA Probes; D011993:Recombinant Fusion Proteins; D005696:Galactosidases