Stimulation of thymus cells by the calcium ionophore A23187 in the presence of LPS and 2-ME: on the mode of action of A23187 and 2-ME.
The stimulation of murine spleen and thymus cells by the calcium ionophore A23187 was studied using a serum-free culture technique. In cultures of spleen cells, rabbit IgG was capable of replacing A23187 in bringing about the stimulation of cells. Low concentrations of A23187 were inhibitory to proliferating B lymphocytes. In cultures of thymus cells a stimulating activity of A23187 was observed only if LPS was present in the medium.
Burger
M
M
Institute of Pathology, University of Bern, Switzerland.
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology; D000001:Calcimycin / Q000494:pharmacology*; D002478:Cells, Cultured; D003469:Culture; D005260:Female; D005978:Glutathione / Q000494:pharmacology; D007074:Immunoglobulin G / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008213:Lymphocyte Activation; D008623:Mercaptoethanol / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D013950:Thymus Gland / Q000166:cytology*
D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D000001:Calcimycin; D008623:Mercaptoethanol; D005978:Glutathione