X-ray diffraction analyses of crystals of rat liver NAD(P)H:(quinone-acceptor) oxidoreductase containing cibacron blue.
NAD(P)H:(quinone-acceptor) oxidoreductase (EC 1.6.99.2) is a widely distributed dicoumarol-inhibitable FAD-containing protein that catalyzes the obligatory two-electron reduction of quinones. The enzyme plays an important role in protecting animal cells against quinone toxicity and may be involved in the vitamin K-dependent blood coagulation cascade. Cocrystallization of rat liver quinone reductase with Cibacron blue, a potent inhibitor with respect to NAD(P)H, was achieved by the method of vapor diffusion in the presence of ammonium sulfate and low concentrations of polyethylene glycol. X-ray diffraction analysis showed these blue crystalline platelets to be monoclinic and to belong to the space group P2(1) (a = 71.6 A, b = 107.1 A, c = 87.8 A and beta = 92.60 degrees) with two dimers in the asymmetric unit. The crystals diffract to a resolution of at least 2.8 A.
Ysern
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Department of Biophysics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Prochaska
H J
HJ
D000645:Ammonium Sulfate; D000818:Animals; D003460:Crystallization; D008099:Liver / Q000201:enzymology*; D046911:Macromolecular Substances; D016660:NAD(P)H Dehydrogenase (Quinone); D011092:Polyethylene Glycols; D011808:Quinone Reductases / Q000037:antagonists & inhibitors; D051381:Rats; D014227:Triazines / Q000494:pharmacology; D014961:X-Ray Diffraction
D046911:Macromolecular Substances; D014227:Triazines; D011092:Polyethylene Glycols; C012842:Cibacron Blue F 3GA; D016660:NAD(P)H Dehydrogenase (Quinone); D011808:Quinone Reductases; D000645:Ammonium Sulfate