Starch gel electrophoresis of lactate dehydrogenase and glucose phosphate isomerase of poultry coccidia using the LKB multiphor.
A modification of thin-layer starch gel horizontal electrophoresis is described. The original method of Wraxall and Culliford (1986) is improved so that the preparation of starch gel is as simple as preparing the agarose gel. Thus the commercially supplied kits and instruments for the agarose gel electrophoresis can be also used for the starch gel electrophoresis. Furthermore, a method of preparing the permanent dry enzymograms from the starch gels with visualized enzymes is presented. The described procedure was used in the LDH and GPI analyses of poultry coccidia.
Kucera
J
J
Research Institute of Feed Supplements and Veterinary Drugs, Jílové, Prague, Czechoslovakia.
D000818:Animals; D004539:Eimeria / Q000201:enzymology*; D004592:Electrophoresis, Starch Gel; D005956:Glucose-6-Phosphate Isomerase / Q000032:analysis*; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D011200:Poultry
D007770:L-Lactate Dehydrogenase; D005956:Glucose-6-Phosphate Isomerase