[The humoral immune response to the influenza virus: the characteristics of the enriched virus-neutralizing fraction of hyperimmune mouse serum].
Certain characteristics of immunosorbents prepared on the basis of aminocellulose and oxycellulose were determined. The affinity chromatography of sera from influenza-virus-immunized mice was shown to yield with the former of the immunosorbents a fraction of antibody in which the relative portion of antibodies detectable by neutralization test was higher than that in the original hyperimmune serum. The preparation of pure antibodies obtained with the oxycellulose-based immunosorbent contained antibodies detectable by NT and HI test in a ratio similar to that in the unfractionated hyperimmune serum. It is concluded that aminocellulose and oxycellulose differ in the nature of chemical interaction with influenza virion proteins owing to which differences in the pattern of interaction of immunosorbents prepared on their basis with virus-specific antibodies are observed. The process of influenza virion neutralization by antibodies was also shown to be a multi-hit reaction, the ratio of NT- and HI-detectable antibodies in a preparation affecting the kinetics of the virus-neutralizing effect of the substance. An increase in the preparation of a portion of NT-detectable antibodies reduced the time in which the number of antibody molecules sufficient for virus particle neutralization is adsorbed to it.
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D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis* / Q000302:isolation & purification; D000917:Antibody Formation; D002482:Cellulose / Q000032:analysis*; D002846:Chromatography, Affinity / Q000379:methods; D007106:Immune Sera / Q000032:analysis* / Q000302:isolation & purification; D005779:Immunodiffusion / Q000379:methods; D007136:Immunoglobulins / Q000032:analysis; D007163:Immunosorbent Techniques; D007164:Immunosorbents; D009980:Influenza A virus / Q000276:immunology*; D051379:Mice; D009500:Neutralization Tests / Q000379:methods
D000914:Antibodies, Viral; D007106:Immune Sera; D007136:Immunoglobulins; D007164:Immunosorbents; D002482:Cellulose
Gumoral'nyĭ immunnyĭ otvet na virus grippa: kharakteristika obogashchennoĭ virus-neĭtralizuiushcheĭ fraktsii giperimmunnoĭ syvorotki mysheĭ.