title: Plant regeneration of mesophyll protoplasts from axenic potato (Solanum tuberosum L.) shoot.
abstract: Mesophyll protoplasts from commercial potatoes were cultured in shallow liquid media. The regenerated cells divided and formed calli. Regenerant plants of two potato lines (Ke Xin 4; 68-62) were obtained from the regenerated calli after transfer to solid medium. The division frequency of the regenerated cells was influenced by culture protoplast density. Growth of the protoplast-derived colonies was sensitive to sucrose concentration in the liquid medium. The protoplast culture medium obviously affected differentiation frequency for the protoplast-derived calli (P-calli). The P-callus differentiation frequency was raised by supplementing the medium with 3% mannitol, which also shortened the P-callus differentiation.
authors.affiliation: Institute of Genetics, Academia Sinica, Beijing.
authors.forename: S J
authors.initials: SJ
authors.lastname: Li
authors.forename: X H
authors.initials: XH
authors.lastname: Li
authors.forename: Y R
authors.initials: YR
authors.lastname: Sun
mesh_terms: D002478:Cells, Cultured; D009024:Morphogenesis; D009994:Osmolar Concentration; D011523:Protoplasts / Q000166:cytology / Q000502:physiology*; D012038:Regeneration / Q000502:physiology*; D011198:Solanum tuberosum / Q000166:cytology / Q000502:physiology*; D013395:Sucrose / Q000494:pharmacology
chemical_list: D013395:Sucrose