The poly-beta-hydroxybutyrate granule in vivo. A new insight based on NMR spectroscopy of whole cells.
High resolution 13C NMR spectroscopy of live cells has been used to show that poly-beta-hydroxybutyrate (PHB) is predominantly in a mobile state within the storage granules of Alcaligenes eutrophus, Methylobacterium extorquens, and Methylobacterium AM1. Comparison of chemical and NMR analysis of PHB indicates that about 70% of the polymer in A. eutrophus gives sharp observable resonances. Temperature-dependent line widths and relaxation rates together with nuclear Overhauser effect measurements demonstrate that the observed material is effectively a mobile amorphous elastomer that is well above its glass transition temperature. The hydroxyvalerate-hydroxybutyrate copolymer produced by propionate-fed A. eutrophus has virtually the same mobility as the homopolymer. Evidence is presented indicating that water is an integral component of the PHB granule and that this component acts as a plasticizer for the polymer. These observations strongly suggest that the enzyme(s) responsible for PHB biosynthesis and consumption operate only on mobile hydrated material and that the solid granules characteristic of dried cells are partially artifactual. This model is supported by a reinterpretation of previously inexplicable biochemical results.
Barnard
G N
GN
University Chemical Laboratory, Cambridge, United Kingdom.
Sanders
J K
JK
D000421:Alcaligenes / Q000737:chemistry* / Q000648:ultrastructure; D055598:Chemical Phenomena; D002621:Chemistry; D003080:Cold Temperature; D003594:Cytoplasmic Granules / Q000737:chemistry; D005615:Freezing; D006088:Gram-Negative Aerobic Bacteria / Q000737:chemistry* / Q000648:ultrastructure; D006885:Hydroxybutyrates / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D011091:Polyesters / Q000032:analysis*; D011108:Polymers; D000094122:Polyhydroxybutyrates
D006885:Hydroxybutyrates; D011091:Polyesters; D011108:Polymers; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates