Electrophoretic pattern of blood and cuticular phenoloxidase of different crustaceans.
A 7% sodium dodecyl sulfate (SDS) polyacrylamide slab gel electrophoresis was carried out to study the molecular weight of blood and cuticular phenoloxidase from different crustaceans. In Uca lacteus de Haan, Scylla serrata Forskal and Ocypode platytarsis Milne Edwards, the apparent molecular weight of cuticular phenoloxidase is higher than that of blood phenoloxidase, whereas the molecular weights of cuticular phenoloxidase isozymes of Emerita emeritus L. are lower than those of blood phenoloxidase isozymes. In Metapenaeus monoceros Fabricius, the blood phenoloxidase shows a molecular weight of 250,000 and the cuticular phenoloxidase shows two isozymes with molecular weights of 350,000 and 250,000. The individuality of blood phenoloxidase and cuticular phenoloxidase and their roles are discussed.
Nellaiappan
K
K
Department of Zoology, University of Madras, India.
Vinayagam
A
A
Kalyani
R
R
D000818:Animals; D004156:Catechol Oxidase / Q000097:blood*; D003445:Crustacea / Q000201:enzymology*; D003743:Dental Enamel / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D014442:Monophenol Monooxygenase / Q000097:blood*
D004156:Catechol Oxidase; D014442:Monophenol Monooxygenase