Cytogenetic response to 1,2-dicarbonyls and hydrogen peroxide in Chinese hamster ovary AUXB1 cells and human peripheral lymphocytes.
Mutagenic 1,2-dicarbonyls have been reported to occur in coffee and other beverages and in various foods. We have measured the induction of sister-chromatid exchanges (SCEs) and endoreduplicated cells (ERCs) to determine the genotoxicity of various 1,2-dicarbonyl compounds in Chinese hamster ovary (CHO) AUXB1 cells and human peripheral lymphocytes. The 1,2-dicarbonyls glyoxal, methylglyoxal and kethoxal each induced highly significant increases in both SCEs and ERCs in AUXB1 cells. Glyoxal and kethoxal induced SCEs but not ERCs in human peripheral lymphocytes. In addition, hydrogen peroxide induced highly significant levels of SCEs and ERCs in AUXB1 cells. Bisulfite, which reacts with carbonyl groups to form addition products, significantly reduced the frequency of SCEs and the proportion of ERCs when glyoxal, methylglyoxal, kethoxal and diacetyl were administered to AUXB1 cells. In addition, bisulfite blocked the formation of ERCs, but not SCEs, induced by hydrogen peroxide. These in vitro results suggest that 1,2-dicarbonyls may play an important role in the genotoxicity of some foods and beverages.
Tucker
J D
JD
Lawrence Livermore National Laboratory, University of California 94550.
Taylor
R T
RT
Christensen
M L
ML
Strout
C L
CL
Hanna
M L
ML
Carrano
A V
AV
D000447:Aldehydes / Q000633:toxicity*; D000818:Animals; D002074:Butanones; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D003931:Diacetyl / Q000633:toxicity; D004171:Diploidy; D006037:Glyoxal / Q000633:toxicity; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D007659:Ketones / Q000633:toxicity*; D008214:Lymphocytes / Q000187:drug effects*; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D009153:Mutagens; D011765:Pyruvaldehyde / Q000633:toxicity; D012854:Sister Chromatid Exchange / Q000187:drug effects; D013447:Sulfites / Q000494:pharmacology
D000447:Aldehydes; D002074:Butanones; D007659:Ketones; D008937:Mitomycins; D009153:Mutagens; D013447:Sulfites; D006037:Glyoxal; D016685:Mitomycin; D011765:Pyruvaldehyde; D006861:Hydrogen Peroxide; C005135:kethoxal; D003931:Diacetyl; C009279:sodium bisulfite