title: Maternal and embryonic transcripts of Drosophila proto-oncogenes are expressed in Schneider 2 culture cells but not in l(2)gl transformed neuroblasts.
abstract: The transcription patterns of Drosophila melanogaster src, abl and two ras homologs were analyzed in normal Drosophila tissue, in neuroblasts derived from tumorous larval brain of the mutant lethal(2)giant larvae [l(2)gl] and in Schneider 2 tissue culture cells. Our results show that, in addition to constitutive transcripts, the src, abl, ras1 and ras3 genes express a set of maternal/embryonic-specific transcripts. By using these transcripts as differentiation markers we show that, in spite of their embryonic-like, undifferentiated phenotype, the l(2)gl transformed neuroblasts are authentic larval cells. Using the same criterion the Schneider 2 tissue culture cells show the characteristics of embryonic cells.
authors.affiliation: Department of Biology, Technion-Israel Institute of Technology, Haifa.
authors.forename: Z
authors.initials: Z
authors.lastname: Kimchie
authors.forename: O
authors.initials: O
authors.lastname: Segev
authors.forename: Z
authors.initials: Z
authors.lastname: Lev
mesh_terms: D000818:Animals; D002460:Cell Line; D004247:DNA; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics*; D004625:Embryo, Nonmammalian; D005260:Female; D005786:Gene Expression Regulation; D009474:Neurons / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D011519:Proto-Oncogenes; D014407:Tumor Cells, Cultured / Q000378:metabolism*
chemical_list: D004247:DNA