The effect of MHC compatibility between parasite-infected cell line and recipient in immunization against tropical theileriosis.
Lymphoblastoid cell lines, infected and transformed in vitro by a Moroccan stock of Theileria annulata, infected and immunized susceptible taurine cattle, at cell doses of 10(8), 10(6), 10(4) and 10(2), regardless of whether the recipients were BoLA matched or mismatched to the donor cell line. The MHC relationship between the cell line and recipient did affect the severity of the clinical response to cell line immunization which may reflect differences in the specific priming of the immune response. At the highest cell doses the BoLA-mismatched recipients reacted more severely than the BoLA-matched. This study shows that, unlike the closely related parasite T. parva, there is no histocompatibility barrier to immunization using T. annulata-infected cell lines which could be achieved with as few as 10(2) allogeneic infected cells. The role of MHC compatibility between cell line and recipient in the priming of a protective immune response is discussed.
Innes
E A
EA
Institute of Animal Physiology and Genetics Research, Edinburgh Research Station.
Ouhelli
H
H
Oliver
R A
RA
Simpson
S P
SP
Brown
C G
CG
Spooner
R L
RL
D000818:Animals; D016782:Apicomplexa / Q000276:immunology; D002417:Cattle; D002460:Cell Line; D015395:Histocompatibility Antigens Class I; D007114:Immunization; D008214:Lymphocytes / Q000276:immunology / Q000469:parasitology; D013801:Theileriasis / Q000276:immunology / Q000517:prevention & control*
D015395:Histocompatibility Antigens Class I