title: Microtubule-associated protein tau. Identification of a novel peptide from bovine brain.
abstract: The microtubule-associated protein tau isolated from bovine brain was cleaved with CNBr and the 3 largest peptides of approx. 21, 19 and 18 kDa were obtained. Dephosphorylation of the CNBr digest of tau with alkaline phosphatase changed the electrophoretic mobility of these peptides to 19, 18 and 17 kDa. Amino acid sequencing of the total CNBr digest of tau revealed at least 3 sequences, two of which were highly homologous to previously published mouse and human tau sequences derived from cDNAs. A third amino acid sequence of 17 residues with heterogeneity at position 11 showed no homology with the cDNA-derived tau sequences. These studies suggest that the amino acid sequences of mammalian tau predicted from their cDNAs might be incomplete.
authors.affiliation: New York State Institute for Basic Research in Developmental Disabilities, Staten Island, NY 10314.
authors.forename: K
authors.initials: K
authors.lastname: Iqbal
authors.forename: A J
authors.initials: AJ
authors.lastname: Smith
authors.forename: T
authors.initials: T
authors.lastname: Zaidi
authors.forename: I
authors.initials: I
authors.lastname: Grundke-Iqbal
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D003488:Cyanogen Bromide; D004247:DNA / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010766:Phosphorylation; D013045:Species Specificity; D016875:tau Proteins
chemical_list: D000596:Amino Acids; D008869:Microtubule-Associated Proteins; D016875:tau Proteins; D004247:DNA; D003488:Cyanogen Bromide