Scanning microfluorometry in intravital microvascular research.
Our own development of fluorometric scanning techniques in intravital microscopy of the microcirculation is described. Very tiny amount of fluorometric substances are detected with a high temporal and locational resolution. The everted small intestinal mesentery of the rat serves as a model. We have given a detailed description of the microscopes used, the optical systems, the conditions of measurement of the microfluorometry, the scanning techniques and the evaluation of the measurement data. The present state of technical development detects 10(-12) g of a fluorochromed plasma protein in 8 ms in a measurement field of 2 microns 2. The four-digit measurement data of a scanning line of 200 microns length in 0.25 micron locational resolution are registered in about 2 s.
Witte
S
S
Dept. of Medicine, Diakonissen-Hospital, Karlsruhe-Rüppurr, Federal Republic of Germany.
D000818:Animals; D001798:Blood Proteins / Q000032:analysis*; D003592:Cytophotometry / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D008643:Mesentery / Q000098:blood supply; D008833:Microcirculation; D051381:Rats; D012709:Serum Albumin / Q000032:analysis*; D013861:Thiocyanates
D001798:Blood Proteins; D005452:Fluoresceins; D012709:Serum Albumin; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate