Pseudomonas aeruginosa extracellular toxicity in infant mice and the protective effect of antitoxin A.
The toxin A-content of crude extracellular preparations from Pseudomonas aeruginosa has been measured by ELISA. Using infant mice as test animal, the toxicity of these preparations was evaluated. The LD 50 in infant mice was determined at 80 ng of purified toxin A in saline. With ten microliters of rabbit antitoxin A serum given together with purified toxin, the LD 50 increased to 2,500 ng. Generally there was no correlation between the LD 50 of the extracellular preparations, their quantity of toxin A as measured by ELISA, and the protective effect of antitoxin A.
Ogaard
A R
AR
Norwegian Defence Microbiological Laboratory, National Institute of Public Health, Oslo.
Berdal
B P
BP
D036002:ADP Ribose Transferases; D000818:Animals; D000992:Antitoxins / Q000494:pharmacology; D001427:Bacterial Toxins; D004797:Enzyme-Linked Immunosorbent Assay; D005098:Exotoxins / Q000032:analysis* / Q000633:toxicity; D007928:Lethal Dose 50; D051379:Mice; D011550:Pseudomonas aeruginosa / Q000472:pathogenicity*; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D000992:Antitoxins; D001427:Bacterial Toxins; D005098:Exotoxins; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A