Visualization of anionic sites using polyethyleneimine-metal complexes.
17 Polyethyleneimine-metal complexes were synthesized and 3 of them were tested cytochemically for visualization of negative tissue charges. The demonstration of the anionic sites was carried out on rat cerebral cortex and on frog cutaneous pectoral muscle. As controls, neuraminidase digestion, methylation, and omission of osmium-postfixation were used. The osmiophilic properties of polyethyleneimine, polyethyleneimine salts, and polyethyleneimine-metal complexes were discussed.
Pfüller
U
U
Staatliches Institut für Immunpräparate und Nährmedien, Berlin, GDR.
Ichev
K
K
Ovtscharoff
W
W
Franz
H
H
D000818:Animals; D000838:Anions; D002462:Cell Membrane / Q000648:ultrastructure*; D002540:Cerebral Cortex / Q000648:ultrastructure*; D007202:Indicators and Reagents; D008670:Metals; D008854:Microscopy, Electron; D009132:Muscles / Q000648:ultrastructure*; D011094:Polyethyleneimine; D011898:Ranidae; D051381:Rats; D012519:Sarcoplasmic Reticulum / Q000648:ultrastructure; D013572:Synaptic Vesicles / Q000648:ultrastructure
D000838:Anions; D007202:Indicators and Reagents; D008670:Metals; D011094:Polyethyleneimine