title: Genomic structure of murine beta-1,4-galactosyltransferase.
abstract: We have isolated a series of overlapping murine genomic DNA clones that include the complete coding sequence of the Golgi membrane bound marker enzyme beta-1,4-galactosyltransferase. The coding sequence is distributed into six exons spanning 50,000 b.p. of mouse chromosome 4. The COOH terminal domain is predominantly encoded by exons 2-6 and the transmembrane and amino terminal cytoplasmic domains are encoded by exon 1. S1 analysis establishes the most 5' transcriptional initiation site 190 b.p. upstream of the first methionine residue.
authors.affiliation: Monsanto Co., St. Louis, MO 63198.
authors.forename: G F
authors.initials: GF
authors.lastname: Hollis
authors.forename: J G
authors.initials: JG
authors.lastname: Douglas
authors.forename: N L
authors.initials: NL
authors.lastname: Shaper
authors.forename: J H
authors.initials: JH
authors.lastname: Shaper
authors.forename: J M
authors.initials: JM
authors.lastname: Stafford-Hollis
authors.forename: R J
authors.initials: RJ
authors.lastname: Evans
authors.forename: I R
authors.initials: IR
authors.lastname: Kirsch
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005700:Galactosyltransferases / Q000235:genetics*; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D005700:Galactosyltransferases