Comparison of interleukin 1 release and interleukin 1 mRNA expression of human monocytes activated by bacterial lipopolysaccharide or synthetic lipid A.
Several of the biological effects of bacterial lipopolysaccharide (LPS) can be induced by the lipid A part of the molecule. Here we show that in human peripheral blood monocytes, synthetic E. coli lipid A is as effective as the whole LPS molecule in inducing the production of interleukin 1 (IL-1) bioactivity which remains associated to the cells (i.e. IL-1 alpha). In contrast, LPS- but not lipid A-stimulated cells released the bioactive IL-1 produced into the culture supernatant (mainly IL-1 beta). Northern blotting analysis demonstrated, however, that LPS and lipid A are equally effective in inducing the accumulation of IL-1 alpha and IL-1 beta mRNA. These data support the hypothesis that induction of IL-1 biosynthesis and activation of the secretory mechanism for IL-1 are independent phenomena.
Hurme
M
M
Department of Bacteriology and Immunology, University of Helsinki, Finland.
Serkkola
E
E
D002478:Cells, Cultured; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000235:genetics; D008050:Lipid A / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis*
D007375:Interleukin-1; D008050:Lipid A; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D007371:Interferon-gamma