Heparin inhibits the "quasi-lipoxygenase" activity of hemoglobin toward linoleic acid by oxidant scavenging.
Previous studies have shown heparin to have antiinflammatory properties. We have attempted to determine if the mechanism involves the inhibition of lipid oxidation by utilizing a model system where linoleic acid is oxidized in the presence of oxygen and methemoglobin. Heparin inhibits this "quasi-lipoxygenase" activity prolonging the lag phase and slowing the rate of lipid peroxidation. An oxidant scavenging mechanism is also inferred from the fact that heparin is capable of inhibiting luminol-dependent chemiluminescence resulting from the reduction of 15-HPETE by methemoglobin. It is concluded that heparin, in at least this model system, is capable of inhibiting lipoxygenation by an oxidant scavenging mechanism.
Brestel
E P
EP
Department of Medicine, University School of Medicine, Morgantown, WV 26506.
Koes
M T
MT
D006493:Heparin / Q000494:pharmacology*; D015289:Leukotrienes / Q000378:metabolism; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism*; D008054:Lipid Peroxides / Q000378:metabolism; D016859:Lipoxygenase Inhibitors; D008163:Luminescent Measurements; D008353:Mannitol / Q000494:pharmacology; D008706:Methemoglobin / Q000378:metabolism*; D008956:Models, Chemical; D010084:Oxidation-Reduction / Q000187:drug effects
D015289:Leukotrienes; D008041:Linoleic Acids; D008054:Lipid Peroxides; D016859:Lipoxygenase Inhibitors; D008353:Mannitol; C025086:15-hydroperoxy-5,8,11,13-eicosatetraenoic acid; D006493:Heparin; D008706:Methemoglobin; D019787:Linoleic Acid