Cell cycle related change of Ara-C transport in HL-60 cells after differentiation induction.
Using a promyelocytic leukemia cell line, HL-60, we studied the membrane transport of Ara-C before and after differentiation induced by retinoic acid (RA). In RA-treated cells, Ara-C transport was reduced and there was a concomitant increase of the ID50 values of Ara-C in comparison with the controls. By three different procedures to synchronize untreated cells, i.e. density arrest G1 phase enrichment, aphidicolin-induced S phase accumulation and the double isoleucine block method, we found that Ara-C transport was 30-50% higher in the S phase than in the G1 phase. Therefore, the observed decrease in Ara-C transport is, in part, due to the retarded growth accompanied by an accumulation of cells in the G1 phase after differentiation induction.
Takimoto
T
T
Department of Pediatrics, Kyoto University, Japan.
Kubota
M
M
Tsuruta
S
S
Kitoh
T
T
Tanizawa
A
A
Akiyama
Y
Y
Mikawa
H
H
D016590:Aphidicolin; D001692:Biological Transport; D002453:Cell Cycle; D002454:Cell Differentiation / Q000187:drug effects; D002462:Cell Membrane / Q000378:metabolism; D003561:Cytarabine / Q000378:metabolism*; D003842:Deoxycytidine Kinase / Q000235:genetics / Q000378:metabolism; D004224:Diterpenes / Q000494:pharmacology; D006801:Humans; D007399:Interphase / Q000187:drug effects; D015473:Leukemia, Promyelocytic, Acute; D009154:Mutation; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D004224:Diterpenes; D003561:Cytarabine; D016590:Aphidicolin; D014212:Tretinoin; D003842:Deoxycytidine Kinase