Characterization of amplified intracisternal A-particle elements encoding integrase.
Type IIB intracisternal A-particle (IAP) elements have undergone marked amplification and transposition in the genomic DNA of some mouse myelomas. We have made a cDNA library from one such myeloma, MOPC 315, to determine whether some property of the elements themselves has a role in this process. Sequencing of several type IIB cDNAs and one genomic type IIB IAP element has shown that they are nearly identical (greater than 99%) and contain 2 open reading frames (ORFs). ORF2 is capable of encoding the IAP integrase, an enzyme which catalyzes integration of proviral DNA into the genome. An antiserum to a synthetic peptide based on the IAP integrase gene sequence reacted with ORF2 product expressed in bacteria as a fusion protein, and detected a 47 kDa protein, predicted from the size of ORF2, in myeloma cell fractions by Western blotting.
Lueders
K K
KK
Laboratory of Biochemistry, National Cancer Institute, Bethesda, MD 20892.
Grossman
Z
Z
Fewell
J W
JW
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D004254:DNA Nucleotidyltransferases / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D004622:Embryo, Mammalian; D005784:Gene Amplification; D015723:Gene Library; D007426:Genes, Intracisternal A-Particle; D019426:Integrases; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010954:Plasmacytoma / Q000235:genetics; D014176:Protein Biosynthesis; D011519:Proto-Oncogenes; D015183:Restriction Mapping; D014158:Transcription, Genetic
D004273:DNA, Neoplasm; D004254:DNA Nucleotidyltransferases; D019426:Integrases