Abilities of activated macrophages to manifest tumoricidal activity and to generate reactive nitrogen intermediates: a comparative study in vitro and ex vivo.
The abilities of lymphokines and heat-killed bacteria to induce and to maintain tumoricidal activity and/or the secretion of reactive nitrogen intermediates (RNI) were comparatively assessed in bone marrow-derived mononuclear phagocytes (BMM phi) in vitro and in adherent peritoneal cells (APC) ex vivo. In showing that the kinetics of tumoricidal activity and of secretion of RNI induced by macrophage-activating agents in BMM phi and/or in peritoneal cells do largely parallel each other, the present findings provide evidence for a role of RNI in tumor cell killing by activated macrophages both in vitro and in vivo.
Keller
R
R
Institute of Immunology and Virology, University of Zurich, Switzerland.
Keist
R
R
D000818:Animals; D001854:Bone Marrow Cells; D002460:Cell Line; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D007371:Interferon-gamma / Q000494:pharmacology; D007700:Kinetics; D008089:Listeria monocytogenes / Q000276:immunology; D008222:Lymphokines / Q000494:pharmacology; D008262:Macrophage Activation; D008264:Macrophages / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D012515:Mast-Cell Sarcoma / Q000276:immunology; D051379:Mice; D009573:Nitrites / Q000378:metabolism*; D010586:Phagocytes / Q000166:cytology / Q000276:immunology; D011425:Propionibacterium acnes / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D011994:Recombinant Proteins
D008222:Lymphokines; D009573:Nitrites; D011994:Recombinant Proteins; D007371:Interferon-gamma