[Studies on endotoxin of Leptospira. V. The effect of EDTA-Na on the release of the LPS of Leptospira interrogans serovar lai].
The yield of leptospiral LPS was able to increase 1-2 times when the cells were treated with EDTA-Na before extracting with hot phenal-water method. The colours and solubility of L-LPS were improved. When the leptospiral cells were stored for a long period of time, and treated by EDTA-Na, the phase distribution of the LPS changed, i.e. they returned from water phase into phenol phase. Such change was related to the quantity of both the saccharide and lipid.
Wu
S H
SH
Jiang
S X
SX
Wang
H Q
HQ
Nie
D K
DK
Zhu
G F
GF
D004492:Edetic Acid / Q000494:pharmacology*; D007921:Leptospira interrogans / Q000187:drug effects / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000378:metabolism*
D008070:Lipopolysaccharides; D004492:Edetic Acid