Urokinase-type plasminogen activator biosynthesis is induced by the EJ-Ha-ras oncogene in CL26 mouse colon carcinoma cells.
CL26 murine colon carcinoma cells express urokinase-type plasminogen activator (u-PA) mRNA and activity after transfection with the activated c-Ha-ras-I (EJ-ras) oncogene cloned from the EJ bladder carcinoma. PA activity and mRNA in control cells transfected with the non-mutated c-Ha-ras-I (CO-ras) gene remained negative. Ras mRNA was detected in EJ-ras- and CO-ras-transfected cells, but not in untransfected or pSV2-neo-transfected cells. These results indicate that u-PA biosynthesis can be modulated by EJ-Ha-ras-dependent pathways of signal transduction.
Testa
J E
JE
Department of Experimental Pathology, Swiss Institute for Experimental Cancer Research ISREC, Epalinges.
Medcalf
R L
RL
Cajot
J F
JF
Schleuning
W D
WD
Sordat
B
B
D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D002478:Cells, Cultured; D003110:Colonic Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D004790:Enzyme Induction; D004792:Enzyme Precursors / Q000096:biosynthesis / Q000235:genetics*; D011905:Genes, ras; D051379:Mice; D010957:Plasmids; D010960:Plasminogen Activators / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection; D014568:Urokinase-Type Plasminogen Activator / Q000096:biosynthesis / Q000235:genetics*
D004792:Enzyme Precursors; D012333:RNA, Messenger; D010960:Plasminogen Activators; D014568:Urokinase-Type Plasminogen Activator