title: Bacereutin, an antifungal antibiotic isolated from metabolites of Bacillus cereus CHU 130.
abstract: An antifungal metabolite, bacereutin, was isolated from culture filtrate of Bacillus cereus CHU 130. The bacterium was isolated from soils collected in Changhwa County, Taiwan, and was grown in soybean meal-mannitol broth for production of the antibiotic metabolite. The antibiotic metabolite was isolated by adsorption column chromatography of Amberite XAD-2 and was purified by passing through the chromatographic columns packed with Dowex 50W-X8, Sephadex LH 20 and Biogel P-2. The antibiotic metabolite was soluble in water and 87% acetone, and was slightly soluble in methanol, but was not dissolved in n-propanol, n-butanol, acetone, benzene and ethyl acetate. The antibiotic metabolite was a heat-stable and ninhydrin-positive substance. The antibiotic activities of bacereutin were tested by means of the agar-diffusion plate method. The antibiotic metabolite inhibited the growth of Saccharomyces cerevisiae CHU 1, Paecilomyces variotii CHU 6, Rhizomucor miehei CHU 40 and Fusarium oxysportum CHU 98. Bacereutin was a ninhydrin-positive antifungal antibiotic.
authors.affiliation: Department of Botany, National Chung-Hsing University, Taichung, Taiwan, Republic of China.
authors.forename: J S
authors.initials: JS
authors.lastname: Tschen
authors.forename: C N
authors.initials: CN
authors.lastname: Tseng
mesh_terms: D000096:Acetone; D000935:Antifungal Agents / Q000302:isolation & purification* / Q000494:pharmacology; D001409:Bacillus cereus / Q000378:metabolism*; D002845:Chromatography; D004586:Electrophoresis; D005670:Fusarium / Q000187:drug effects; D000432:Methanol; D009090:Mucorales / Q000187:drug effects; D009555:Ninhydrin; D009930:Organic Chemicals; D010143:Paecilomyces / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000187:drug effects; D012995:Solubility; D013194:Staining and Labeling; D014867:Water
chemical_list: D000935:Antifungal Agents; D009930:Organic Chemicals; D014867:Water; C064474:bacereutin; D000096:Acetone; D009555:Ninhydrin; D000432:Methanol