Determination of acetoacetate in urine by solid-phase spectrophotometry.
A method for the determination of acetoacetate has been developed based on solid-phase spectrophotometry (SPS). The acetoacetate reacts with nitroprusside and glycine and the reaction product is sorbed on Dowex 1-X8 resin. The absorbance of the resin phase at 590 and 720 nm is measured directly. The calibration graph is linear up to 3.3 mg l-1 and the RSD is 1.9%. The detection limit is 7.6 micrograms l-1. The method has been applied to the determination of acetoacetate in normal and diabetic subjects' urine without pretreatment of the samples, and the results compared with those of 1H-NMR and homogeneous nitroprusside methods.
Molina
F
F
Department of Analytical Chemistry, University of Granada, Spain.
Valencia
M C
MC
de la Torre
J
J
Capitán-Vallvey
L F
LF
D000090:Acetoacetates / Q000652:urine*; D002138:Calibration; D003920:Diabetes Mellitus / Q000652:urine*; D005998:Glycine; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007477:Ions; D007657:Ketone Bodies / Q000652:urine*; D008027:Light; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009599:Nitroprusside; D010710:Phosphates; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods
D000090:Acetoacetates; D007477:Ions; D007657:Ketone Bodies; D010710:Phosphates; D009599:Nitroprusside; C016635:acetoacetic acid; D005998:Glycine