title: Effects of infusions of various doses of bovine growth hormone-releasing factor on blood hormones and metabolites in lactating Holstein cows.
abstract: In two experiments, the effects of i.v. infusions of various doses of bovine GH-releasing factor (GRF) on blood hormones and metabolites in lactating Holstein cows were determined. In experiment 1, cows were infused with GRF (0, 3.125, 6.25, 12.5, 25.0 or 50.0 mg/cow per 24 h) for 24 h. Blood was sampled at -1, 5, 11, 15 and 23 h relative to the start of the infusion. The serum concentration of somatomedin C (SM-C) before infusion was 303 +/- 8 (S.E.M.) micrograms/l. Doses of GRF of between 3.125 and 50.0 mg were equipotent in stimulating (P less than 0.05) SM-C by 1.5- to 2.5-fold. GRF-induced increases in SM-C occurred by 11 h from the start of the infusion. In experiment 2, primiparous cows were infused with GRF (0, 1 or 3 mg/24 h) for 20 days. Blood was sampled for 12 h on days 1, 10 and 19. The 1 mg dose of GRF increased (P less than 0.05) blood concentrations of SM-C (on days 10 and 19) and glucose (on day 19), but did not affect blood concentrations of prolactin, insulin, cortisol, tri-iodothyronine (T3), thyroxine (T4), non-esterified fatty acids (NEFA) or glucose. The 3 mg dose of GRF increased (P less than 0.05) blood concentrations of SM-C (on days 10 and 19), T3 (on days 10 and 19), insulin (on day 19), NEFA (on days 1, 10 and 19) and glucose (on day 19), but did not affect blood concentrations of prolactin, cortisol or T4. We conclude that these data are consistent with the hypothesis that the galactopoietic effect of exogenous GRF in dairy cattle is mediated by increased secretion of GH.
authors.affiliation: Department of Animal Science, Michigan State University, East Lansing 48824.
authors.forename: W J
authors.initials: WJ
authors.lastname: Enright
authors.forename: L T
authors.initials: LT
authors.lastname: Chapin
authors.forename: W M
authors.initials: WM
authors.lastname: Moseley
authors.forename: S A
authors.initials: SA
authors.lastname: Zinn
authors.forename: M B
authors.initials: MB
authors.lastname: Kamdar
authors.forename: L F
authors.initials: LF
authors.lastname: Krabill
authors.forename: H A
authors.initials: HA
authors.lastname: Tucker
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002417:Cattle / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D013006:Growth Hormone / Q000097:blood; D013007:Growth Hormone-Releasing Hormone / Q000008:administration & dosage / Q000494:pharmacology*; D007328:Insulin / Q000097:blood; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D007774:Lactation / Q000378:metabolism*; D011247:Pregnancy; D013974:Thyroxine / Q000097:blood; D013997:Time Factors; D014284:Triiodothyronine / Q000097:blood
chemical_list: D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D014284:Triiodothyronine; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone; D013007:Growth Hormone-Releasing Hormone; D013974:Thyroxine