Nitroreductase activity of NADH dehydrogenase of the respiratory redox chain.
1. An NADH-dependent nitroreductase from the inner membrane of ox liver mitochondria copurified with Complex I of the respiratory redox chain (NADH:ubiquinone oxidoreductase, EC 1.6.5.3). 2. The corresponding nitroreductase from ox heart mitochondria co-purified with the NADH-cytochrome c reductase of Mahler, Sarkar & Vernon [(1952) J. Biol. Chem. 199, 585-597] [NADH: (acceptor) oxidoreductase, EC 1.6.99.3], a component of Complex I that contains the FMN. 3. The mitochondrial nitroreductase activity is attributed to the flavoprotein component of Complex I.
Smyth
G E
GE
Department of Biochemistry, Trinity College, Dublin, Ireland.
Orsi
B A
BA
D000818:Animals; D002417:Cattle; D003579:Cytochrome Reductases / Q000378:metabolism*; D046911:Macromolecular Substances; D008566:Membranes / Q000378:metabolism; D008930:Mitochondria, Liver / Q000201:enzymology*; D009243:NAD / Q000378:metabolism*; D016660:NAD(P)H Dehydrogenase (Quinone); D009245:NADH Dehydrogenase / Q000378:metabolism*; D009601:Nitroreductases / Q000378:metabolism*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism*; D011808:Quinone Reductases / Q000378:metabolism
D046911:Macromolecular Substances; D009243:NAD; D010088:Oxidoreductases; D003579:Cytochrome Reductases; D016660:NAD(P)H Dehydrogenase (Quinone); D011808:Quinone Reductases; D009245:NADH Dehydrogenase; D009601:Nitroreductases