ADP-ribosylation of cell membrane proteins by staphylococcal alpha-toxin and leukocidin in rabbit erythrocytes and polymorphonuclear leukocytes.
Staphylococcal alpha-toxin resulted in ADP-ribosylation of the 37 and 41 kDa proteins of a membrane preparation from rabbit erythrocytes. In the presence of 100 microM GTP, the toxin ADP-ribosylated proteins of 54 and 59 kDa and potentiated ADP-ribosylation of the 37 and 41 kDa forms. GTP had no effect on ADP-ribosylation of membrane proteins in the absence of alpha-toxin. Incubation of a membrane preparation of rabbit polymorphonuclear leukocytes with the S and F components of staphylococcal leukocidin resulted in ADP-ribosylation of the 37 and 41 kDa proteins, respectively. Furthermore, the 37, 41, 54 and 59 kDa proteins were ADP-ribosylated by leukocidin in the presence of GTP. The ADP-ribosylation of these proteins was observed to be dependent on the incubation time and toxin dose and was abolished by prior boiling. Addition of agmatine did not attenuate ADP-ribosylation of these proteins. These results demonstrate that staphylococcal alpha-toxin and leukocidin possess ADP-ribosyltransferase activities which are potentiated by GTP and suggest that ADP-ribosylation reactions are responsible for development of the cytolytic activities of these staphylococcal toxins.
Kato
I
I
Second Department of Microbiology, Chiba University School of Medicine, Japan.
Noda
M
M
D000376:Agmatine / Q000494:pharmacology; D000818:Animals; D001427:Bacterial Toxins / Q000378:metabolism / Q000494:pharmacology*; D004357:Drug Synergism; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000494:pharmacology; D006460:Hemolysin Proteins; D007956:Leukocidins / Q000494:pharmacology*; D008565:Membrane Proteins / Q000378:metabolism*; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D011817:Rabbits
D001427:Bacterial Toxins; D006460:Hemolysin Proteins; D007956:Leukocidins; D008565:Membrane Proteins; C021091:staphylococcal alpha-toxin; D000376:Agmatine; D006160:Guanosine Triphosphate; D011065:Poly(ADP-ribose) Polymerases