Comparison of radiometric and microbiological assays for the determination of folate in fortified gruel and porridge.
The results obtained by a radiometric assay of folate in fortified gruel and porridge, using a commercially available radioassay kit, were compared with those obtained by a standard microbiological assay with Lactobacillus casei. A lower level of folate was found with the radiometric assay. Comparison of the results obtained with the two methods gave a correlation coefficient of 0.991 when the analysis was performed on samples extracted at the same time. A lower correlation coefficient was obtained between the result if the extractions were carried out on different occasions. The radiometric assay is a acceptable method for the routine determination of folate in fortified gruel and porridge.
Osterdahl
B G
BG
Swedish National Food Administration, Uppsala.
Johansson
E
E
D002523:Edible Grain / Q000032:analysis*; D005069:Evaluation Studies as Topic; D005492:Folic Acid / Q000032:analysis*; D005527:Food, Fortified / Q000032:analysis*; D006801:Humans; D007225:Infant Food / Q000032:analysis*; D007780:Lacticaseibacillus casei / Q000378:metabolism; D011874:Radiometry / Q000379:methods; D011933:Reagent Kits, Diagnostic
D011933:Reagent Kits, Diagnostic; D005492:Folic Acid