[Production of interleukin 1 during tumor growth].
The production of IL-1 by splenic mononuclear adherent cells (MAC) from BALB/c mice inoculated with Sarcoma 180 (S180) was examined as a possible mechanism underlying the immunosuppression observed in tumor bearing mice. Two different inducers of IL-1 were used to stimulate MAC. A natural polysaccharide PCj3 (1, 5, 10 micrograms/ml) and a lipopolysaccharide (LPS) of E. coli (1, 5, 10, 20 micrograms/ml). The IL-1 activity was assayed by the capacity of the supernatants of MAC cultures in different dilutions (1/5, 1/10, 1/20) to enhance the mitogenic response of murine thymocytes to phytohemagglutinin (PHA). Both stimulants induced comparable levels of IL-1 in normal and 10 day tumor bearing mice. Normal MAC were able to elaborate IL-1 spontaneously in the presence of 1% FCS: the optimum LPS concentration was 20 micrograms/ml in the 1/5 dilution. With regard to PCj3, the optimum concentration was 5 and 10 micrograms/ml in the 1/5 dilution (p less than 0.001 vs control). The maximum activity of IL-1 for MAC of 10 day tumor bearing mice was given by the concentration of 5 micrograms/ml for both stimulants. When MAC were incubated with LPS, the IL-1 production was dose dependent while PCj3 seemed to have reached a saturation level between 5 and 10 micrograms/ml. The increase in tumor size (day 20-30) was associated with a significant decrease in IL-1 production by MAC in response to both stimulants. Therefore, the immunosuppression associated with the late stages of tumor growth may be due to inhibition of IL-1 production.
Chasseing
N A
NA
Medina
P D
PD
Lederkremer
R M
RM
Couto
A
A
Rumi
L S
LS
D000818:Animals; D004926:Escherichia coli; D007108:Immune Tolerance; D007375:Interleukin-1 / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000378:metabolism* / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D011134:Polysaccharides / Q000378:metabolism* / Q000494:pharmacology; D012510:Sarcoma 180 / Q000378:metabolism / Q000473:pathology*; D013154:Spleen / Q000473:pathology
D007375:Interleukin-1; D008070:Lipopolysaccharides; D011134:Polysaccharides
Producción de interleuquina 1 durante el crecimiento tumoral.