Induction of c-fos by an anti-epidermal growth factor receptor monoclonal antibody.
The effect of epidermal growth factor (EGF) or a monoclonal antibody to the human EGF receptor on c-fos and c-myc gene expression was examined in KB epidermoid carcinoma cells. EGF increased c-fos transcripts within 30 min and c-myc transcripts within 6-24 hours. The antibody transiently increased c-fos expression within 30 minutes, but did not alter expression of c-myc. Cycloheximide potentiated the c-fos induction by both EGF and antibody. C-fos induction by antibody was less consistent, and was of briefer duration and lesser intensity than the induction by EGF. Nuclear transcription of c-fos decreased in cells treated with EGF or antibody, indicating that c-fos induction by EGF or antibody resulted from post-transcriptional mechanisms.
Taetle
R
R
Department of Pathology, University of California, San Diego 92103.
Castagnola
J
J
MacLeod
C
C
D000911:Antibodies, Monoclonal; D015152:Blotting, Northern; D002467:Cell Nucleus / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000494:pharmacology; D066246:ErbB Receptors / Q000276:immunology*; D005786:Gene Expression Regulation; D006801:Humans; D007624:KB Cells; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics; D016760:Proto-Oncogene Proteins c-fos; D016271:Proto-Oncogene Proteins c-myc; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D014158:Transcription, Genetic / Q000187:drug effects
D000911:Antibodies, Monoclonal; D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D004815:Epidermal Growth Factor; D066246:ErbB Receptors