kappa B elements strongly activate gene expression in non-lymphoid cells and function synergistically with NF1 elements.
kappa B elements have been described as lymphoid-specific transcriptional activators. Here we show that kappa B elements are able to stimulate expression from test promoters more than 100-fold in T47D and 3T3 non-lymphoid cells. We also demonstrate that nuclear proteins from T47D cells form two prominent complexes with HIV kappa B sites. Since the complexes formed in nuclear extracts from T47D and PHA/PMA stimulated Jurkat cells comigrate in polyacrylamide gels, we suggest that the respective binding protein in T47D cells is either NF-kappa B or a closely related family member. In addition we provide evidence that NF1 and kappa B elements can act synergistically to further increase transcriptional activity.
Hennighausen
L
L
Laboratory of Biochemistry and Metabolism, National Institutes of Health, Bethesda, MD 20892.
Furth
P A
PA
Pittius
C W
CW
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D005803:Genes, Immunoglobulin; D006367:HeLa Cells / Q000276:immunology; D006801:Humans; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014162:Transfection
D007145:Immunoglobulin kappa-Chains