Dietary saturated or polyunsaturated fat and copper deficiency in the rat.
Dietary fat-type and copper (Cu) deficiency have been independently identified as potentially important factors in the etiology of ischemic heart disease (IHD); a disease that has been linked to inflammation and oxygen free radical (OFR) mediated damage. Group (n = 6) of male, weanling, Wistar rats were provided ad libitum with deionized water and control or low Cu diets containing (200 g/kg) either saturated or polyunsaturated fatty acids (SFA or PUFA, respectively) for 56 d. Measurement of several indices of Cu status indicated that both groups fed the low Cu diets were Cu-deficient. SFA consumption resulted in significantly increased hepatic Cu (p less than 0.001) and iron (Fe) (p less than 0.001) concentrations and xanthine oxidase activity (p less than 0.05) and significantly decreased hepatic glucose-6-phosphate dehydrogenase activity (p less than 0.001). Although Cu deficiency resulted in significantly decreased hepatic copper-zinc superoxide dismutase (CuZnSOD) activity (p less than 0.01), no significant effect on the activities of the other hepatic antioxidant enzymes, manganese superoxide dismutase, catalase, and glutathione peroxidase, or glutathione reductase, were observed. Cu deficiency also resulted in significantly decreased hepatic Cu levels (p less than 0.001) and cytochrome c oxidase activity (p less than 0.01). No significant difference in hepatic thiobarbituric acid reactive substances (TBARS), a measure of lipid peroxidation, was found between groups consuming SFA or PUFA, but both Cu-deficient groups exhibited significantly increased hepatic TBARS (p less than 0.001), compared to controls. This was probably owing to the significantly decreased hepatic CuZnSOD activity observed in the Cu-deficient, compared to control animals.
Lynch
S M
SM
Biomedical Sciences Research Centre, University of Ulster, Jordanstown, Newtownabbey, Northern Ireland.
Strain
J J
JJ
D000818:Animals; D003300:Copper / Q000172:deficiency* / Q000378:metabolism; D004032:Diet; D004041:Dietary Fats / Q000494:pharmacology*; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006128:Growth / Q000187:drug effects; D007501:Iron / Q000378:metabolism; D008099:Liver / Q000032:analysis / Q000254:growth & development / Q000378:metabolism; D008297:Male; D008903:Minerals / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013054:Spectrophotometry, Atomic; D013482:Superoxide Dismutase / Q000378:metabolism; D014969:Xanthine Oxidase / Q000378:metabolism; D015032:Zinc / Q000378:metabolism
D004041:Dietary Fats; D004042:Dietary Fats, Unsaturated; D008903:Minerals; D003300:Copper; D007501:Iron; D005954:Glucosephosphate Dehydrogenase; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase; D015032:Zinc