Effect of gonadal steroids on gonadotropin-releasing hormones stimulated human chorionic gonadotropin release by trophoblast cells.
The effect of gonadal steroids on basal and GnRH-stimulated hCG release was studied using collagenase-dispersed trophoblast cells from early pregnancy. Both GnRH and a GnRH superagonist, Buserelin, stimulated hCG release with a similar dose dependency. Progesterone (0.1 to 10 micrograms/ml) inhibited GnRH-stimulated hCG release in a dose dependent manner as well as basal hCG release. Relatively high concentrations of estradiol (10 micrograms/ml) stimulated both basal and GnRH-mediated hCG release and antagonized the inhibitory effect of progesterone on hCG release at 1 micrograms/ml as well as RU486 (1 microgram/ml). These results indicate that progesterone has an important role in both basal and GnRH-mediated hCG regulatory system in the placenta.
Iwashita
M
M
Maternal and Perinatal Center, Tokyo Women's Medical College, Japan.
Watanabe
M
M
Adachi
T
T
Ohira
A
A
Shinozaki
Y
Y
Takeda
Y
Y
Sakamoto
S
S
D002064:Buserelin / Q000494:pharmacology; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000378:metabolism*; D004958:Estradiol / Q000502:physiology; D004963:Estrenes / Q000494:pharmacology; D005260:Female; D012739:Gonadal Steroid Hormones / Q000502:physiology*; D006801:Humans; D015735:Mifepristone; D010906:Pituitary Hormone-Releasing Hormones / Q000037:antagonists & inhibitors / Q000502:physiology*; D011247:Pregnancy; D011374:Progesterone / Q000037:antagonists & inhibitors / Q000502:physiology; D014327:Trophoblasts / Q000378:metabolism*
D006063:Chorionic Gonadotropin; D004963:Estrenes; D012739:Gonadal Steroid Hormones; D010906:Pituitary Hormone-Releasing Hormones; D015735:Mifepristone; D011374:Progesterone; D004958:Estradiol; D002064:Buserelin