Deoxyribonucleic acid-binding properties of human monoclonal anti-platelet antibodies obtained from patients with idiopathic thrombocytopenic purpura.
Recent studies of human monoclonal lupus autoantibodies have revealed that some of them are cross-reactive with platelets. To clarify further the bivalencies in anti-DNA and anti-platelet autoantibodies, we established nine human B cell lines producing monoclonal anti-platelet antibodies from patients with idiopathic thrombocytopenic purpura by Epstein-Barr virus transformation and studied the DNA-binding properties of these antibodies. Three of the antibodies showed cross-reactivity with single-stranded DNA (ssDNA), as determined by enzyme-linked immunosorbent assay and one of them was also found to bind to the synthetic polynucleotides; poly (dT) and poly (I), as well as to ssDNA. These findings suggest that cross-reaction between anti-DNA and anti-platelet antibodies is based on overlaps in specificity existing as common part of both autoantibodies.
Matsumoto
M
M
First Department of Internal Medicine, School of Medicine, Ehime University, Japan.
Mochizuki
K
K
Kobayashi
Y
Y
D000974:Antibodies, Antinuclear / Q000096:biosynthesis / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology; D001323:Autoantibodies / Q000096:biosynthesis / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D001792:Blood Platelets / Q000276:immunology*; D002461:Cell Line, Transformed; D003429:Cross Reactions / Q000276:immunology; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000276:immunology; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000096:biosynthesis / Q000276:immunology; D007075:Immunoglobulin M / Q000096:biosynthesis; D007145:Immunoglobulin kappa-Chains / Q000096:biosynthesis / Q000276:immunology; D011696:Purpura, Thrombocytopenic / Q000276:immunology*
D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D004268:DNA-Binding Proteins; D007147:Immunoglobulin Light Chains; D007075:Immunoglobulin M; D007145:Immunoglobulin kappa-Chains