title: A new 99mTc-red blood cell labeling procedure for cardiac blood pool imaging: clinical results.
abstract: The first clinical results of a new 99mTc-red blood cell labeling procedure avoiding cell centrifugation are presented. One ml heparinized blood samples were incubated with small amounts of a stannous kit. By titration studies, ideal quantities of sodium hypochlorite for oxidation of extracellular tin and of EDTA as stabilizer of the label were found. The Cl- concentration and pH of the labeled blood were acceptable, and EDTA increased labeling yield and stability determined in vitro by a few percent. The new procedure gave a slightly higher labeling yield than a current technique using centrifugation of cells. Labeling efficiency expressed as cell bound/total activity was 96.6% +/- 1.3% in healthy subjects and 95.5% +/- 2.2% in cardiac patients and remained high for 2 h after reinjection. The biological halflife of labeled cells following the new procedure was 11-12 h rendering it suitable for serial determinations of radionuclide cardiography.
authors.affiliation: Department of Clinical Physiology and Nuclear Medicine, Herlev Hospital, Denmark.
authors.forename: H
authors.initials: H
authors.lastname: Kelbaek
authors.forename: K
authors.initials: K
authors.lastname: Bülow
authors.forename: J
authors.initials: J
authors.lastname: Aldershvile
authors.forename: J
authors.initials: J
authors.lastname: Møgelvang
authors.forename: S L
authors.initials: SL
authors.lastname: Nielsen
mesh_terms: D000328:Adult; D000368:Aged; D003327:Coronary Disease / Q000000981:diagnostic imaging; D004492:Edetic Acid; D004912:Erythrocytes; D005260:Female; D006321:Heart / Q000000981:diagnostic imaging*; D006801:Humans; D007553:Isotope Labeling / Q000379:methods; D008297:Male; D008875:Middle Aged; D011877:Radionuclide Imaging; D012973:Sodium Hypochlorite; D013667:Technetium
chemical_list: D013667:Technetium; D004492:Edetic Acid; D012973:Sodium Hypochlorite