Expression of the Escherichia coli beta-glucuronidase gene in industrial and phytopathogenic filamentous fungi.
A chimaeric beta-glucuronidase (GUS) gene has been created by ligating the Aspergillus nidulans glyceraldehyde 3-phosphate dehydrogenase promoter to the coding sequence of the E. coli uidA gene. Co-transformation of this vector into A. nidulans, A. niger and the tomato pathogen Fulvia fulva (syn. Cladosporium fulvum (Cooke] resulted in the expression of beta-glucuronidase. GUS activity was detected by growth on agar media containing X-gluc and by enzyme assays of mycelial extracts. Expression of the gene in F. fulva transformants was also easily detectable during growth in plants and did not affect pathogenicity. These results form the basis for a versatile and sensitive reporter gene system for industrial and phytopathogenic filamentous fungi.
Roberts
I N
IN
Norwich Molecular Plant Pathology Group, School of Biological Sciences, University of East Anglia, UK.
Oliver
R P
RP
Punt
P J
PJ
van den Hondel
C A
CA
D001233:Aspergillus nidulans / Q000235:genetics; D001234:Aspergillus niger / Q000235:genetics; D002963:Cladosporium / Q000235:genetics; D003470:Culture Media; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D005966:Glucuronidase / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D014170:Transformation, Genetic; D001616:beta-Galactosidase / Q000378:metabolism
D003470:Culture Media; D001616:beta-Galactosidase; D005966:Glucuronidase