title: Bioavailability of lysine in selected foods by rat growth assay.
abstract: Lysine bioavailabilities in reference protein and 16 test protein diets were estimated using 10 day rat growth assays. A standard growth curve was obtained by feeding 5 diets containing casein, zein and synthetic amino acids ranging in total lysine concentration from 0.3 to 0.7%. Experimental foods were added to the basal diet at the expense of zein and/or synthetic amino acids to provide 2 specific lysine concentrations, i.e., 0.4 and 0.6%. Availabilities were established by comparing growth responses from the test food diets to the regression line of the standard growth data. Availabilities were over 88% for 13 of 16 products. Utilization was poor in pinto beans (73%), rice-wheat gluten cereal (70%), and skim milk powder heated to 100 degrees C for 12 h (66%). Addition of excess lysine (700 mg/100 g diet) to the pinto bean diet did not improve growth response; thus poor digestibility or some unidentified growth inhibitor is indicated.
authors.affiliation: U.S. Dept. of Agriculture, Beltsville Human Nutrition Research Center, MD 20705.
authors.forename: F E
authors.initials: FE
authors.lastname: McDonough
authors.forename: C E
authors.initials: CE
authors.lastname: Bodwell
authors.forename: A D
authors.initials: AD
authors.lastname: Hitchins
authors.forename: R S
authors.initials: RS
authors.lastname: Staples
mesh_terms: D000818:Animals; D008239:Lysine / Q000008:administration & dosage / Q000032:analysis / Q000378:metabolism*; D008297:Male; D009753:Nutritive Value; D051381:Rats; D011919:Rats, Inbred Strains; D015430:Weight Gain
chemical_list: D008239:Lysine