Rehydratable agarose gels: use of low EEO agarose without charged additives.
Agarose [SeaKem HGT(P)] gels of 0.5% to 1.2% can be dried at room temperature and rehydrated to 84-85% of their original water content. Mobilities, using the 84-85% rehydrated gels, are indistinguishable from those on the original gels. This suggests that the fiber structure and effective pore size of agarose are independent on the 16% loosely bound (and probably synersed) water.
Gombocz
E
E
Section on Macromolecular Analysis, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892.
Chrambach
A
A
D002793:Cholic Acids; D004587:Electrophoresis, Agar Gel; D005612:Freeze Drying; D005990:Glycerol / Q000737:chemistry; D007700:Kinetics; D011137:Polystyrenes / Q000737:chemistry; D013696:Temperature; D014867:Water / Q000737:chemistry
D002793:Cholic Acids; D011137:Polystyrenes; D014867:Water; D005990:Glycerol; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate