A novel enzyme immunoassay for conidia of Fusarium oxysporum f.sp. cucumerinum F504.
This study was undertaken to develop a new tool to study fusarial diseases of plants. Micro- and macro-conidia of a strain (F504) of Fusarium oxysporum were isolated and antiserum against the conidia was elicited in rabbits. A highly specific and sensitive competitive-type enzyme-linked immunosorbent assay (ELISA) for conidia of the strain was developed using the antiserum with beta-D-galactosidase-labeled anti-rabbit IgG as the secondary antibody and conidia fragments of the strain as antigen attached to Amino-Dylark solid-phase balls. The assay was highly specific to conidia of the strain F504, while conidia-free hypha of the strain F504 as well as all other microorganisms tested including nine other strains of Fusarium species showed little cross-reactivity. Application of the ELISA to following the growth rates of conidia in hyphae of the strain F504 under several conditions are also reported.
Kitagawa
T
T
Department of Microbiological Chemistry, Faculty of Pharmaceutical Sciences, Nagasaki University.
Sakamoto
Y
Y
Ogura
H
H
D000818:Animals; D000908:Antibodies, Fungal / Q000276:immunology; D000946:Antigens, Fungal / Q000276:immunology; D001667:Binding, Competitive; D004797:Enzyme-Linked Immunosorbent Assay; D005670:Fusarium / Q000276:immunology*; D007114:Immunization; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000276:immunology; D011817:Rabbits; D001616:beta-Galactosidase / Q000493:pharmacokinetics
D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D007074:Immunoglobulin G; D001616:beta-Galactosidase