title: Isotype analysis of the anti-CENP-B anticentromere autoantibody: evidence for restricted clonality.
abstract: Utilizing the centromere B fusion protein (CENP-B) and specific, matched monoclonal antiisotype reagents in an enzyme-linked immunosorbent assay, we found that anti-CENP-B autoantibodies were skewed to the IgG1 isotype. The overall kappa:lambda light chain ratio was 2:1, although several individual sera showed a strong predominance of one of the light chains. Isoelectric focusing of light chain-skewed sera showed polyclonal patterns. Our findings are consistent with the anti-CENP-B autoantibody response being a chronic, antigen-driven response.
authors.affiliation: Department of Medicine, University of North Carolina, Chapel Hill.
authors.forename: R A
authors.initials: RA
authors.lastname: Eisenberg
authors.forename: W C
authors.initials: WC
authors.lastname: Earnshaw
authors.forename: B J
authors.initials: BJ
authors.lastname: Bordwell
authors.forename: S Y
authors.initials: SY
authors.lastname: Craven
authors.forename: R
authors.initials: R
authors.lastname: Cheek
authors.forename: N F
authors.initials: NF
authors.lastname: Rothfield
mesh_terms: D001323:Autoantibodies / Q000145:classification / Q000276:immunology*; D002503:Centromere / Q000276:immunology*; D002875:Chromosomes / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000145:classification; D007132:Immunoglobulin Isotypes / Q000276:immunology*; D007145:Immunoglobulin kappa-Chains / Q000032:analysis; D007146:Immunoglobulin lambda-Chains / Q000032:analysis; D007526:Isoelectric Point; D012595:Scleroderma, Systemic / Q000276:immunology
chemical_list: D001323:Autoantibodies; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains