An in-vitro assessment of the functional activity of monoclonal anti-D.
The response of human monocytes to red cells sensitized with known levels of monoclonal antibody to the Rh antigen D (anti-D) was compared with that of polyclonal anti-D. Monocyte response was determined by measuring red cell adherence, erythrophagocytosis, monocyte-mediated red cell lysis and luminol-dependent chemiluminescence. By all criteria, monoclonal and polyclonal antibodies showed comparable activity, with IgG3 antibodies promoting a greater monocyte-red cell interaction than IgG1 antibodies. It is suggested that monoclonal anti-D may be effective in the prophylaxis of haemolytic disease of the newborn, providing such material is clinically acceptable.
Hadley
A G
AG
Blood Group Reference Laboratory, Radcliffe Infirmary, Oxford, UK.
Kumpel
B M
BM
Leader
K
K
Merry
A H
AH
Brojer
E
E
Zupanska
B
B
D000911:Antibodies, Monoclonal / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D009000:Monocytes / Q000276:immunology; D010587:Phagocytosis; D012204:Rh-Hr Blood-Group System / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D012204:Rh-Hr Blood-Group System