Pepsin fibrinolysis of artificial clots made from fibrinogen concentrate and bovine thrombin: the effect of pH and epsilon aminocaproic acid.
Artificial clots made from fibrin glue with and without an inhibitor of fibrinolysis can be used to treat gastrointestinal bleeding. We have been unable to find descriptions of the effects of acid and pepsin upon such artificial clots. Therefore, 10(-2) mol/l epsilon aminocaproic acid was added to fibrin glue in vitro at acid concentrations of pH 1.0 and pH 5.5. Pepsin was added at 9000 U/50 ml, the expected value for fasting human subjects. There was a highly significant reduction in clot survival at pH 1.0. At pH 5.5, clot weight was also significantly decreased with pepsin, compared to control. Thus pepsin and acidity greatly affect survival of artificial clots, but the addition of epsilon aminocaproic acid did not affect clot survival.
Valenzuela
G A
GA
Department of Internal Medicine, University of Virginia Health Sciences Center, Charlottesville 22908.
Spotnitz
W D
WD
Stone
D D
DD
D000614:Aminocaproates / Q000494:pharmacology*; D015119:Aminocaproic Acid / Q000494:pharmacology*; D015718:Fibrin Tissue Adhesive; D005342:Fibrinolysis; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D010434:Pepsin A / Q000494:pharmacology*
D000614:Aminocaproates; D015718:Fibrin Tissue Adhesive; D010434:Pepsin A; D015119:Aminocaproic Acid