Auxiliary liver transplantation in jaundiced rats with UDP-glucuronyltransferase deficiency and defective hepatobiliary transport.
In this study auxiliary liver transplantation (ALT) has been tested as a means of correcting the UDP-glucuronyltransferase deficiency in Gunn rats and the UDP-glucuronyltransferase deficiency and impaired hepatobiliary bilirubin transport in double mutant rats. In both groups serum bilirubin normalized and remained low until the end of the study at 12 weeks after transplantation in 4 out of 6 rats. Excretion of 99mTc-HIDA in non-transplanted double mutants was considerably slower than in Gunn rats (kel 0.9 x 10(-3) versus 4.3 x 10(-3) s-1). HIDA excretion by transplants in double mutants and Gunn rats was about equal (kel 1.6 x 10(-3) and 1.1 x 10(-3) s-1). Experiments with bile duct-cannulated transplants showed that in double mutants bile flow, bile acid and bilirubin excretion was 2-4 times higher than in Gunn rats. This study shows that auxiliary liver transplants can conjugate and excrete bilirubin when one of these or both functions are lacking in the recipient's liver.
Jansen
P L
PL
Division of Gastrointestinal and Liver Diseases, Academic Medical Center, Amsterdam, The Netherlands.
Hess
F
F
Peters
W H
WH
Koenders
E
E
Jerusalem
C
C
Corstens
F H
FH
D000818:Animals; D001663:Bilirubin / Q000097:blood; D014453:Glucuronosyltransferase / Q000172:deficiency*; D006498:Hepatectomy; D007565:Jaundice / Q000601:surgery*; D008099:Liver / Q000000981:diagnostic imaging; D016031:Liver Transplantation; D008297:Male; D011877:Radionuclide Imaging; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity
D014453:Glucuronosyltransferase; D001663:Bilirubin