title: Heterogeneity of Mycoplasma iowae determined by restriction enzyme analysis.
abstract: Strains of Mycoplasma iowae were homogeneous in some characteristics and heterogeneous in others. Thus, the biochemical tests, immunofluorescence, and protein profiling by sodium dodecyl sulfate-polyacrylamide gel electrophoresis were group-specific tests. However, some minor differences in protein patterns were seen among strains. The growth inhibition test tended to be strain-specific. Hemagglutination titers were very low and unstable for the majority of strains. One strain (RY-65) with a stable high-titer hemagglutinin failed to react in the hemagglutination-inhibition test against immune sera to the reference strains. Restriction endonuclease DNA analyses was the most useful method to differentiate 1 strain from another.
authors.affiliation: Department of Epidemiology and Preventive Medicine, School of Veterinary Medicine, University of California, Davis 95616.
authors.forename: S H
authors.initials: SH
authors.lastname: Zhao
authors.forename: R
authors.initials: R
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D004269:DNA, Bacterial / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D009174:Mycoplasma / Q000032:analysis / Q000145:classification* / Q000235:genetics; D015183:Restriction Mapping
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial