title: Antioxidant activity of 5-hydroxytryptophan, 5-hydroxyindole, and DOPA against microsomal lipid peroxidation and its dependence on vitamin E.
abstract: The antioxidant capacity of 5-hydroxy-tryptophan, 5-hydroxy-indole, and DOPA (3,4-dihydroxy-phenylalanine) was tested in the Fe-induced lipid peroxidation of liver microsomes of normal- and vitamin E-deficient rats, using ascorbate as a reductant. Lipid peroxidation was monitored as low-level chemiluminescence, indicative of generation of electronically-excited states arising from the recombination of secondary lipid peroxyl radicals. The lag phase (tau 0) preceding the rise in chemiluminescence intensity was used as indicator of antioxidant efficiency, in the absence (tau 0) and the presence (tau) of these polar compounds. The increase in duration of the lag phase exerted by these hydroxy compounds was expressed and quantified as the relationship: tau-tau 0. The tau-tau 0 values were considerably higher in the presence of vitamin E and almost negligible in the absence of tocopherol. It is postulated that the observed increased protection against lipid peroxidation by the above 5-hydroxy derivatives is displayed in a fashion dependent on the presence of vitamin E and probably involving recovery of the chromanoxyl radical by means of an electron-transfer process.
authors.affiliation: Department of Pathology II, University of Linköping, Sweden.
authors.forename: E
authors.initials: E
authors.lastname: Cadenas
authors.forename: M G
authors.initials: MG
authors.lastname: Simic
authors.forename: H
authors.initials: H
authors.lastname: Sies
mesh_terms: D006916:5-Hydroxytryptophan / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants; D004295:Dihydroxyphenylalanine / Q000494:pharmacology*; D007211:Indoles / Q000494:pharmacology*; D007700:Kinetics; D015227:Lipid Peroxidation / Q000187:drug effects*; D008163:Luminescent Measurements; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D012016:Reference Values; D014811:Vitamin E Deficiency / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D007211:Indoles; C026785:5-hydroxyindole; D004295:Dihydroxyphenylalanine; D006916:5-Hydroxytryptophan