title: Effect of hypertonic stress on liver cell volume, bile flow, and volume-regulatory K+ fluxes.
abstract: Net hepatic uptake and release of K+ were studied in the isolated-perfused rat liver subjected to a 10-min period of hyperosmotic stress by addition of 80 mM mannitol or sucrose to the perfusing solution. Bile flow and effluent Na+, K+, and Ca2+ activities were monitored throughout. Upon initiation of hypertonic stress, a sharp transient dilution of effluent ion activities indicated hepatic water losses that were larger and occurred more rapidly with sucrose than with mannitol. During continuous hyperosmotic perfusion, portocaval differences in K+ uncovered a steady net influx of the ion into the liver that reached a higher maximum and led to a greater accumulation in experiments with sucrose compared with mannitol. This hepatic K+ uptake was completely blocked by 1 mM ouabain. Upon return to isotonic conditions, a sharp transient concentration of effluent ion activities suggested hepatic water uptake that was again more rapid and pronounced in sucrose- than mannitol-treated livers. This was followed by a transient phase of net hepatic K+ release whose magnitude and duration were proportional to the water movements induced by the removal of each carbohydrate. Administration of 2 mM Ba2+ abolished this K+ efflux. These results indicate that mannitol equilibrates between extra- and intracellular compartments, whereas sucrose apparently does not. The data also suggest that net movements of K+ may be involved in the regulatory volume responses induced by hyperosmotic stress and return to normal tonicity, respectively.
authors.affiliation: Institute of General and Experimental Pathology, University of Vienna, Austria.
authors.forename: P
authors.initials: P
authors.lastname: Haddad
authors.forename: T
authors.initials: T
authors.lastname: Thalhammer
authors.forename: J
authors.initials: J
authors.lastname: Graf
mesh_terms: D000818:Animals; D001464:Barium / Q000494:pharmacology; D017609:Barium Compounds; D001646:Bile / Q000378:metabolism*; D002712:Chlorides; D006982:Hypertonic Solutions; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008353:Mannitol / Q000494:pharmacology; D010042:Ouabain / Q000494:pharmacology; D010477:Perfusion; D011188:Potassium / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013395:Sucrose / Q000494:pharmacology
chemical_list: D017609:Barium Compounds; D002712:Chlorides; D006982:Hypertonic Solutions; C024986:barium chloride; D001464:Barium; D008353:Mannitol; D013395:Sucrose; D010042:Ouabain; D011188:Potassium