Arachidonic acid metabolism in cultured astrocytes from rat embryo and in C6 glioma cells.
Arachidonic acid metabolism in primary cultures of astroglial cells prepared from cerebra of rat embryos was examined. Arachidonic acid was mainly metabolized through the lipoxygenase pathway and the major metabolites formed were 12-hydroxyeicosatetraenoic acid (12-HETE), 11-HETE and 15-HETE. By contrast, in C6 cells, which are considered to be of astroglial origin, arachidonic acid was mainly metabolized through the cyclooxygenase pathway and the major metabolites formed were prostaglandin (PG)E2, PGF2 alpha and thromboxane B2.
Ishizaki
Y
Y
Section of Physiological Chemistry, Faculty of Dentistry, Tokyo Medical and Dental University, Japan.
Morita
I
I
Murota
S
S
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism*; D001921:Brain / Q000166:cytology / Q000378:metabolism; D002478:Cells, Cultured; D004537:Eicosanoic Acids / Q000378:metabolism*; D005910:Glioma; D051381:Rats; D011919:Rats, Inbred Strains; D014407:Tumor Cells, Cultured / Q000378:metabolism*
D001095:Arachidonic Acids; D004537:Eicosanoic Acids; D016718:Arachidonic Acid