[Methods of isolating phospholipids from Coxiella burnetii].
Phospholipids are a part of the outside membrane of Coxiella burnetii (or other gram negative bacteria). It is possible to extract them with a mixture of chloroform-methanol (CM). For extraction of phospholipids from C. burnetii it is important to take account of temperature, proportion of CM, and reaction time. The best results are obtained at CM 2:1, at boiling point of this mixture and reaction time of 2 hours. Longer reaction time does not influence the reaction, the additional amounts of phospholipids can be extracted only with fresh solvent mixture.
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D002855:Chromatography, Thin Layer; D016997:Coxiella burnetii / Q000378:metabolism*; D006358:Hot Temperature; D008563:Membrane Lipids / Q000302:isolation & purification*; D010743:Phospholipids / Q000302:isolation & purification*; D012997:Solvents
D008563:Membrane Lipids; D010743:Phospholipids; D012997:Solvents
Sposoby izoliatsii fosfolipidov iz koksiell berneta.