title: The carboxy terminus of the viral Jun oncoprotein is required for complex formation with the cellular Fos protein.
abstract: The products of the proto-oncogenes c-jun and c-fos are known to form a complex in vivo. Complex formation appears to stabilize protein-DNA interactions and is thought to play an important functional role in transcriptional regulation. Here we show that the viral Jun oncoprotein, which differs structurally from cellular Jun, is also capable of complex formation with Fos. Thus the oncogenic potency of viral Jun is unlikely to be due to an altered affinity for Fos. We have also defined, by deletion analysis, the domain of v-Jun responsible for complex formation to reside in the carboxy terminus encompassing the leucine zipper motif. We find that complex formation with c-Fos does not occur with v-Jun deletions affecting one or more leucine residues in the zipper domain. Our results are consistent with the hypothesis that the leucine zipper mediates Jun-Fos interaction.
authors.affiliation: Department of Microbiology, University of Southern California School of Medicine, Los Angeles 90033-1054.
authors.forename: T J
authors.initials: TJ
authors.lastname: Bos
authors.forename: F J
authors.initials: FJ
authors.lastname: Rauscher
authors.forename: T
authors.initials: T
authors.lastname: Curran
authors.forename: P K
authors.initials: PK
authors.lastname: Vogt
mesh_terms: D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D009154:Mutation; D016759:Oncogene Protein p65(gag-jun); D014176:Protein Biosynthesis; D011487:Protein Conformation; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D012191:Retroviridae Proteins / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D016759:Oncogene Protein p65(gag-jun); D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D012191:Retroviridae Proteins; D014157:Transcription Factors; D004247:DNA