Rapid fluorogenic assay for differentiation of the Candida parapsilosis group from other Candida spp.
A 30-min fluorogenic test was developed for differentiation of members of the Candida parapsilosis group from other Candida species commonly encountered in clinical material. The fluorogenic substrate, 4-methylumbelliferyl-beta-D-glucoside, was utilized to assay beta-glucosidase activity. A total of 50 C. parapsilosis isolates and 135 isolates of four other Candida species were tested. Assay sensitivity and specificity were 100 and 99.3%, respectively. The procedure was adapted for use with a spectrofluorometer.
Smitka
C M
CM
Laboratory Services Branch, Ontario Ministry of Health, Toronto, Canada.
Jackson
S G
SG
D002175:Candida / Q000145:classification* / Q000201:enzymology / Q000302:isolation & purification; D005959:Glucosidases / Q000032:analysis*; D005960:Glucosides / Q000235:genetics / Q000276:immunology*; D006027:Glycosides / Q000235:genetics / Q000276:immunology; D006923:Hymecromone / Q000031:analogs & derivatives / Q000276:immunology*; D011237:Predictive Value of Tests; D013050:Spectrometry, Fluorescence; D014468:Umbelliferones / Q000031:analogs & derivatives / Q000276:immunology; D001617:beta-Glucosidase / Q000032:analysis*
D005960:Glucosides; D006027:Glycosides; D014468:Umbelliferones; C014110:4-methylumbelliferyl glucoside; D006923:Hymecromone; D005959:Glucosidases; D001617:beta-Glucosidase