title: The rho gene product expressed in E. coli is a substrate of botulinum ADP-ribosyltransferase C3.
abstract: The ras-related rho A protein expressed in E. coli, was ADP-ribosylated by botulinum ADP-ribosyltransferase C3. C3 also modified the valine-14 mutant rho protein but not the products of H-ras, R-ras, ral, ypt, and rap 1 genes. A ras-rho chimaera consisting of 60 amino acids from the amino terminus of ras fused to 133 amino acids from the carboxy terminus of rho was not modified by C3. Antibodies raised against the porcine brain cytosolic substrate of C3 cross reacted with the rho, valine-14 rho and ras-rho proteins, but not with the gene products of H-ras, R-ras, ral or rap 1. Polyclonal anti-H-ras antibodies cross reacted with H-ras but not with ral, rho, or the C3 substrate purified from porcine brain.
authors.affiliation: Rudolf-Buchheim-Institut für Pharmakologie, Giessen, FRG.
authors.forename: K
authors.initials: K
authors.lastname: Aktories
authors.forename: U
authors.initials: U
authors.lastname: Braun
authors.forename: S
authors.initials: S
authors.lastname: Rösener
authors.forename: I
authors.initials: I
authors.lastname: Just
authors.forename: A
authors.initials: A
authors.lastname: Hall
mesh_terms: D036002:ADP Ribose Transferases / Q000378:metabolism*; D001905:Botulinum Toxins; D003014:Clostridium botulinum / Q000201:enzymology; D004926:Escherichia coli / Q000235:genetics*; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D016283:Proto-Oncogene Proteins p21(ras); D011994:Recombinant Proteins / Q000378:metabolism; D013379:Substrate Specificity; D020742:rhoA GTP-Binding Protein
chemical_list: D008565:Membrane Proteins; D011518:Proto-Oncogene Proteins; D011994:Recombinant Proteins; D036002:ADP Ribose Transferases; C063007:exoenzyme C3, Clostridium botulinum; D001905:Botulinum Toxins; D019204:GTP-Binding Proteins; D016283:Proto-Oncogene Proteins p21(ras); D020742:rhoA GTP-Binding Protein