Cooperative effects of gamma-interferon and 1 alpha, 25-dihydroxyvitamin D3 on in vitro differentiation of the blast cells of RAEB and RAEB-T.
We added recombinant human gamma-interferon (gamma-IFN) and 1 alpha, 25-dihydroxyvitamin D3 (1 alpha, 25 (OH)2D3) to the bone marrow cells from six patients with RAEB or RAEB-T in liquid suspension cultures. After cultivation for 7 to 9 days, numerical, morphological and functional changes of the cells were assessed. gamma-IFN and 1 alpha, 25 (OH)2D3 additively suppressed cell growth, especially the number of blast cells decreased. The expression of alpha-naphthylbutyrate esterase (NBE) activity appeared to be promoted but that of naphthol AS-D chloroacetate esterase (NAE) activity was apparently suppressed by the addition of gamma-IFN and/or 1 alpha, 25 (OH)2D3. The percentage of NBT reduction-positive cells and latex-phagocytizing cells was only slightly increased by both agents. These results indicate that gamma-IFN and 1 alpha, 25 (OH)2D3 cooperate to induce monocytoid differentiation of the patients' blast cells. Combination therapy with both agents merits further study.
Tohyama
K
K
First Division of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.
Ohmori
S
S
Ueda
T
T
Ueda
Y
Y
Sakoda
H
H
Yoshida
Y
Y
Uchino
H
H
D000328:Adult; D000368:Aged; D000754:Anemia, Refractory, with Excess of Blasts / Q000473:pathology*; D001853:Bone Marrow / Q000473:pathology; D002117:Calcitriol / Q000494:pharmacology*; D002452:Cell Count / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002648:Child; D004357:Drug Synergism; D005260:Female; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000473:pathology; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D008297:Male; D008863:Microspheres; D008875:Middle Aged; D009580:Nitroblue Tetrazolium; D010587:Phagocytosis / Q000187:drug effects
D009580:Nitroblue Tetrazolium; D007371:Interferon-gamma; D002117:Calcitriol