title: [Determination of bactericidal minimum concentrations of 3 antiseptics and 1 disinfectant on 580 hospital gram-negative bacilli].
abstract: Minimal bactericidal activities (MBCs) of three antiseptics (povidone iodine, chlorhexidine digluconate, benzalkonium chloride) and one disinfectant (sodium hypochloride) where determined, on 580 hospital Gram negative bacilli. Previously the Afnor T 72-150 standard for antiseptic and disinfectant was established for two reference strains E. coli CIP 54 127 and P. aeruginosa CIP A 22. No difference was found between the MBC obtained with these strains in Afnor standard and in microdilution method. Microdilution method allows to test 11 hospital isolates and one reference strain. A strain was considered as resistant when the MBC was one dilution higher than the reference strain MBC. Results were as follows: None strain was resistant to sodium hypochloride and povidone iodine; 18.2% Enterobacteriaceae were resistant to chlorhexidine digluconate with 94.2% of Proteus; 4% of Enterobacteriaceae were resistant to benzalkonium chloride with 89.5% of Proteus and only 1.8% other bacilli. Results obtained in the present study are similar as those previously published particularly with Proteus; nevertheless other studies have reported P. aeruginosa strains resistant to chlorhexidine digluconate and benzalkonium chloride; this last point was not observed in our study.
authors.affiliation: Laboratoire Central de Microbiologie, Hôpital Edouard-Herriot, Lyon.
authors.forename: P
authors.initials: P
authors.lastname: Girardo
authors.forename: M E
authors.initials: ME
authors.lastname: Reverdy
authors.forename: A
authors.initials: A
authors.lastname: Martra
authors.forename: J
authors.initials: J
authors.lastname: Fleurette
mesh_terms: D001548:Benzalkonium Compounds / Q000494:pharmacology*; D002710:Chlorhexidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D006801:Humans; D008826:Microbial Sensitivity Tests; D011205:Povidone / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012973:Sodium Hypochlorite / Q000494:pharmacology*
chemical_list: D001548:Benzalkonium Compounds; D012973:Sodium Hypochlorite; D011205:Povidone; C010882:chlorhexidine gluconate; D002710:Chlorhexidine
vernacular_title: Détermination de la concentration minimale bactéricide de trois antiseptiques et un désinfectant sur 580 souches de bacilles gram négatifs d'origine hospitalière.