Direct evidence for intrastrand DNA inversion of kappa immunoglobulin gene segments in two murine plasmacytomas.
The products of kappa immunoglobulin gene recombination have been characterized in two murine plasmacytomas to examine the relationship between V-J products and reciprocal elements. By cloning, sequencing, hybridization, and application of the polymerase chain reaction, we have established the direct relationship of the kappa recombination products in these cells. The results provide stronger support for the intrastrand mechanism of kappa gene recombination as well as demonstrating a role for secondary, corrective recombinations.
Feddersen
R
R
Department of Biochemistry, University of Minnesota, Minneapolis 55455.
Van Ness
B
B
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D007446:Chromosome Inversion; D003062:Codon / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D005803:Genes, Immunoglobulin; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D008099:Liver / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010954:Plasmacytoma / Q000235:genetics* / Q000276:immunology; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D015183:Restriction Mapping
D003062:Codon; D004273:DNA, Neoplasm; D007145:Immunoglobulin kappa-Chains