Cost-effective assays for use in monitoring carbamazepine, phenobarbital, and phenytoin in serum.
Using Syva "EMIT" reagents and the Cobas Bio centrifugal analyzer, we have developed cost-effective assays for therapeutic drug monitoring of carbamazepine, phenobarbital, and phenytoin. With these optimized methods one can assay up to 2470 samples with a single 100-test EMIT kit with good precision and accuracy. Between-batch CVs, based on results of repeat analysis of routine patients' samples, were 4.6%, 7.0%, and 5.3%, respectively. Results correlated well with those by methods previously used [gas chromatography and fluorescence polarization (Abbott TDx)], but there was a negative bias (-10.9%) of our method for phenobarbital results in patients' samples, as compared with those obtained with the TDx.
Moore
F M
FM
Department of Clinical Chemistry, Royal Infirmary, Edinburgh, Scotland, U.K.
Simpson
D
D
D002220:Carbamazepine / Q000097:blood*; D002849:Chromatography, Gas; D003362:Cost-Benefit Analysis; D015200:Fluoroimmunoassay; D006801:Humans; D007124:Immunoenzyme Techniques; D010634:Phenobarbital / Q000097:blood*; D010672:Phenytoin / Q000097:blood*; D012015:Reference Standards
D002220:Carbamazepine; D010672:Phenytoin; D010634:Phenobarbital