Gamete processing in an environmental chamber: effect on in vitro fertilization outcome.
A study of mouse gamete processing for in vitro fertilization (IVF) under various conditions showed that it is necessary to control the atmosphere if the temperature is raised from 22 degrees C to 37 degrees C. The data suggest that maximum IVF success is attained by processing the gametes at 37 degrees C, under an atmosphere of 5% O2 and 5% CO2, and overlaying the medium with silicone oil.
Gwatkin
R B
RB
Research Institute and Department of Gynecology, Cleveland Clinic Foundation, Ohio 44195-5240.
Quigley
M M
MM
Collins
R L
RL
D000818:Animals; D001273:Atmosphere Exposure Chambers; D002245:Carbon Dioxide / Q000502:physiology; D005314:Embryonic and Fetal Development; D005260:Female; D005307:Fertilization in Vitro; D006813:Humidity; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008954:Models, Biological; D009865:Oocytes / Q000502:physiology*; D010100:Oxygen / Q000502:physiology; D010313:Partial Pressure; D013094:Spermatozoa / Q000502:physiology*; D013696:Temperature
D002245:Carbon Dioxide; D010100:Oxygen