Immune regulation of the L5178Y murine tumor-dormant state: induction of cell- and soluble factor-mediated inhibition of tumor cell growth by tumor necrosis factor and gamma-interferon.
Small concentrations of recombinant murine tumor necrosis factor (rMuTNF) synergized with recombinant murine gamma-interferon (rMuIFN-gamma) to inhibit tumor cell growth in peritoneal cell (PC) cultures from tumor-dormant mice. A soluble inhibitor of tumor cell growth was produced in the rMuTNF-treated PC cultures, but there was no synergistic enhancement of production of this inhibitor by the addition of rMuIFN-gamma. Treatment of both plastic-adherent PC and nonadherent PC cultures which contained L5178Y cells with rMuTNF + rMuIFN-gamma resulted in inhibition of tumor cell growth. However, in the absence of L5178Y cells, rMuTNF + rMuIFN-gamma induced antitumor cytotoxic activity in the plastic-adherent but not in the plastic nonadherent PC. These results indicate that the antitumor activity of rMuTNF + rMuIFN-gamma in PC from tumor-dormant mice involves both cell- and soluble factor-mediated mechanisms.
Suzuki
Y
Y
Department of Pathology and Laboratory Medicine, Hahnemann University, Philadelphia, Pennsylvania 19102.
Chen
L P
LP
Liu
C M
CM
Morita
T
T
Wheelock
E F
EF
D000818:Animals; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D004357:Drug Synergism; D005260:Female; D007371:Interferon-gamma / Q000494:pharmacology*; D051379:Mice; D008811:Mice, Inbred DBA; D009374:Neoplasms, Experimental / Q000276:immunology* / Q000473:pathology; D010529:Peritoneal Cavity / Q000276:immunology; D011994:Recombinant Proteins / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma