title: Bovine lens aldose reductase: identification of two enzyme forms.
abstract: Two structurally different forms of bovine lens aldose reductase have been identified. Freshly prepared lens extracts contain an unactivated "b form" (ARb) which is sensitive to inhibition by Sorbinil. Upon incubation of the extracts with oxygen radical generating systems, ARb is converted to a more active "a form" (ARa), which is not inhibited by Sorbinil. ARa and ARb were purified to electrophoretic homogeneity.
authors.affiliation: Department of Physiology and Biochemistry, University of Pisa, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Del Corso
authors.forename: D
authors.initials: D
authors.lastname: Barsacchi
authors.forename: M
authors.initials: M
authors.lastname: Camici
authors.forename: D
authors.initials: D
authors.lastname: Garland
authors.forename: U
authors.initials: U
authors.lastname: Mura
mesh_terms: D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D000818:Animals; D002417:Cattle; D002845:Chromatography / Q000379:methods; D004492:Edetic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000494:pharmacology; D048289:Imidazolidines; D007501:Iron / Q000494:pharmacology; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D007908:Lens, Crystalline / Q000201:enzymology*; D013401:Sugar Alcohol Dehydrogenases / Q000302:isolation & purification*; D014020:Tissue Extracts / Q000032:analysis
chemical_list: D007093:Imidazoles; D048289:Imidazolidines; D007527:Isoenzymes; D014020:Tissue Extracts; D004492:Edetic Acid; D007501:Iron; D013401:Sugar Alcohol Dehydrogenases; D000449:Aldehyde Reductase; C026411:sorbinil