title: Effect of flow rate on tissue plasminogen activator activity in human parotid saliva.
abstract: Parotid saliva from 10 healthy volunteers was collected at rest and at constant flow rates of 0.25, 0.5 and 1.0 ml/min, and its tissue plasminogen activator (tPA) activity assayed on fibrin plates containing plasminogen. In unstimulated salivas the median tPA activity was 0.26 (range 0.03-2.0) IU/ml. During the first 15 min of stimulation, a continuous decrease in tPA activity was found at the three flow rates; thereafter a steady state was obtained. No significant differences in activity were found between the three rates. The initial decrease was on average 0.15 IU/ml, and the activity during the steady state was 38% of the prestimulatory level. Thus, stimulation with citric acid causes a significant decrease in tPA activity of parotid saliva but this decrease, as well as the reduction rate, appears to be independent of flow rate.
authors.affiliation: Department of Cariology, School of Dentistry, Karolinska Institute, Huddinge, Stockholm, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Kjaeldgaard
authors.forename: F
authors.initials: F
authors.lastname: Lagerlöf
authors.forename: A
authors.initials: A
authors.lastname: Kjaeldgaard
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D010306:Parotid Gland / Q000378:metabolism*; D012212:Rheology; D012463:Saliva / Q000032:analysis* / Q000378:metabolism; D013268:Stimulation, Chemical; D013997:Time Factors; D010959:Tissue Plasminogen Activator / Q000032:analysis*
chemical_list: D010959:Tissue Plasminogen Activator