Structural and functional exchangeability of 5 S RNA species from the eubacterium E.coli and the thermoacidophilic archaebacterium Sulfolobus solfataricus.
The role of 5 S RNA within the large ribosomal subunit of the extremely thermophilic archaebacterium Sulfolobus solfataricus has been analysed by means of in vitro reconstitution procedures. It is shown that Sulfolobus 50 S subunits reconstituted in the absence of 5 S RNA are inactive in protein synthesis and lack 2-3 ribosomal proteins. Furthermore, it has been determined that in the course of the in vitro assembly process Sulfolobus 5 S RNA can be replaced by the correspondent RNA species of E.coli; Sulfolobus reconstituted particles containing the eubacterial 5 S molecule are stable and active in polypeptide synthesis at high temperatures.
Teixidò
J
J
Centro de Biologia Molecular, UAM-CSIC, Madrid, Spain.
Altamura
S
S
Londei
P
P
Amils
R
R
D001105:Archaea / Q000502:physiology*; D018407:Bacterial Physiological Phenomena; D004926:Escherichia coli / Q000502:physiology*; D012335:RNA, Ribosomal / Q000502:physiology*; D012338:RNA, Ribosomal, 23S / Q000502:physiology; D012341:RNA, Ribosomal, 5S / Q000378:metabolism / Q000502:physiology*; D012269:Ribosomal Proteins / Q000302:isolation & purification; D012270:Ribosomes / Q000378:metabolism; D013329:Structure-Activity Relationship
D012335:RNA, Ribosomal; D012338:RNA, Ribosomal, 23S; D012341:RNA, Ribosomal, 5S; D012269:Ribosomal Proteins