Factors affecting 1H NMR spectra of blood plasma: cancer, diet and freezing.
Single pulse and Hahn spin-echo 400 and 500 MHz 1H NMR spectra from normal subjects and cancer patients are reported. No significant difference was found between the average -CH3 and -(CH2)n- lipoprotein linewidths of the two groups in contrast to a previous report. Such measurements are shown to be complicated by freezing and thawing of samples which broadens lipoprotein resonances, and by eating which sharpens them. Deconvolution of the composite lipoprotein signals enables these effects to be interpreted. Other notable features of plasma spectra are discussed including resonances for 'acute-phase' glycoproteins, ketone bodies and other small molecules.
Bell
J D
JD
Department of Chemistry, Birkbeck College, University of London, Christopher Ingold Laboratories, UK.
Brown
J C
JC
Norman
R E
RE
Sadler
P J
PJ
Newell
D R
DR
D000209:Acute-Phase Proteins / Q000378:metabolism; D001800:Blood Specimen Collection; D004032:Diet; D005215:Fasting; D005615:Freezing; D006801:Humans; D006859:Hydrogen; D007657:Ketone Bodies / Q000097:blood; D008074:Lipoproteins / Q000097:blood*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009369:Neoplasms / Q000097:blood*; D012016:Reference Values
D000209:Acute-Phase Proteins; D007657:Ketone Bodies; D008074:Lipoproteins; D006859:Hydrogen