Comparative evaluation of various immunodiagnostic tests for the diagnosis of Taenia solium cysticercosis in pigs, using fractionated antigens.
The sensitivity and specificity of double immunodiffusion (DID), indirect haemagglutination test (IHA), immunoelectrophoresis (IEP), counterimmunoelectrophoresis (CIEP) and enzyme-linked immunosorbent assay (ELISA) were evaluated and compared using saline extracted of Taenia solium larval scolex and its Sephadex G-200 fractionated 1st and 2nd peak as antigens. Various immunodiagnostic tests gave different results with different antigens. Highest sensitivity (92.5%) was obtained with 84.6% sensitivity was obtained with IHA and CIEP respectively using scolex antigen. CIEP gave better results as compared to IEP. Crude antigen gave high sensitivity but less specificity. It was concluded that CIEP can be used as a field test for the anti-mortem diagnosis and ELISA can be employed for laboratory confirmation of T. solium cysticercosis in pigs using fractionated 1st peak antigen.
Kumar
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Department of Parasitology, College of Veterinary Sciences, G. B. Pant University of Agriculture & Technology, Nainital, India.
Gaur
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D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis*; D000947:Antigens, Helminth / Q000276:immunology*; D003378:Counterimmunoelectrophoresis; D003429:Cross Reactions; D003551:Cysticercosis / Q000175:diagnosis / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay; D006386:Hemagglutination Tests; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D011237:Predictive Value of Tests; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*; D013621:Taenia / Q000276:immunology*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth