Detection of a homologue to an E. coli glutamate synthase gene in a cyanobacterium.
The gltBY locus of E. coli, which codes for the two subunits of pyridine nucleotide dependent glutamate synthase, was used as a probe to detect homologues in genomic DNA of Synechococcus PCC6301, a unicellular cyanobacterium. Non-overlapping probes from gltB detected a single homologue with extensive homology, however gltY probes do not detect a strong homologue. The possibility that the gltB homologue encodes a ferredoxin-dependent glutamate synthase of the type found in cyanobacteria, algae and plants is discussed.
Lightfoot
D A
DA
Department of Genetics, University of Leeds, U.K.
McPherson
M J
MJ
Wootton
J C
JC
D000458:Cyanobacteria / Q000201:enzymology* / Q000235:genetics; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005970:Glutamate Synthase / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D013045:Species Specificity; D000637:Transaminases / Q000235:genetics*
D015342:DNA Probes; D004269:DNA, Bacterial; D004247:DNA; D005970:Glutamate Synthase; D000637:Transaminases