[Experimental method for studying conformational transitions in DNA].
An experimental method combining fiber X-ray and direct fiber dimension measurements is proposed for the study of DNA conformational transitions. Curves corresponding to the A-B and B-C transitions are obtained by using the proportionality which exists between the fiber length and the axial rise per nucleotide in the DNA helix. The A-B transition is shown to be cooperative while the B-C one is a progressive change of helical conformation.
Albiser
G
G
Laboratoire de Biophysique moléculaire, U.A. C.N.R.S. n. 494, Université de Nancy-I, Faculté des Sciences, Vandoeuvre-lès-Nancy.
Harmouchi
M
M
Premilat
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S
D004278:DNA, Superhelical; D005336:Fiber Optic Technology; D006813:Humidity; D009690:Nucleic Acid Conformation; D014961:X-Ray Diffraction / Q000379:methods
D004278:DNA, Superhelical
Une méthode expérimentale pour l'étude des transitions de formes de l'ADN.