title: Sequence, structure and evolution of the gene coding for sn-glycerol-3-phosphate dehydrogenase in Drosophila melanogaster.
abstract: We present the complete nucleotide and deduced amino acid sequence for the gene encoding Drosophila sn-glycerol-3-phosphate dehydrogenase. A transcription unit of 5kb was identified which is composed of eight protein encoding exons. Three classes of transcripts were shown to differ only in the 3'-end and to code for three protein isoforms each with a different C-terminal amino acid sequence. Each transcript is shown to arise through the differential expression of three isotype-specific exons at the 3'-end of the gene by a developmentally regulated process of 3'-end formation and alternate splicing pathways of the pre-mRNA. In contrast, the 5'-end of the gene is simple in structure and each mRNA is transcribed from the same promoter sequence. A comparison of the organization of the Drosophila and murine genes and the primary amino acid sequence between a total of four species indicates that the GPDH gene-enzyme system is highly conserved and is evolving slowly.
authors.affiliation: Department of Genetics, North Carolina State University, Raleigh 27695-7614.
authors.forename: G C
authors.initials: GC
authors.lastname: Bewley
authors.forename: J L
authors.initials: JL
authors.lastname: Cook
authors.forename: S
authors.initials: S
authors.lastname: Kusakabe
authors.forename: T
authors.initials: T
authors.lastname: Mukai
authors.forename: D L
authors.initials: DL
authors.lastname: Rigby
authors.forename: G K
authors.initials: GK
authors.lastname: Chambers
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D004331:Drosophila melanogaster / Q000235:genetics*; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics*; D007527:Isoenzymes / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D011817:Rabbits; D014158:Transcription, Genetic
chemical_list: D007527:Isoenzymes; D005993:Glycerolphosphate Dehydrogenase