Comparison of lithium chloride-phenylethanol-moxalactam and modified Vogel Johnson agars for detection of Listeria spp. in retail-level meats, poultry, and seafood.
The effectiveness of Modified Vogel Johnson agar and lithium chloride-phenylethanol-moxalactam agar for detection of Listeria spp. in foods was compared by using the media to analyze retail-level meat, poultry, and seafood both by direct plating and in conjunction with a three-tube most-probable-number enrichment. The most-probable-number protocol detected Listeria species, including Listeria monocytogenes, in a substantial portion of the fresh meat and seafood samples. In most instances the Listeria levels were less than 2 CFU/g, which precluded detection by direct plating. Modified Vogel Johnson agar performed as well as did lithium chloride-phenylethanol-moxalactam agar and was considerably easier to use because of its ability to differentiate Listeria spp. from other microorganisms.
Buchanan
R L
RL
Eastern Regional Research Center, U.S. Department of Agriculture, Philadelphia, Pennsylvania 19118.
Stahl
H G
HG
Bencivengo
M M
MM
Del Corral
F
F
D000818:Animals; D002417:Cattle; D003470:Culture Media; D005399:Fishes; D005516:Food Microbiology; D008087:Listeria / Q000254:growth & development / Q000302:isolation & purification*; D008089:Listeria monocytogenes / Q000254:growth & development / Q000302:isolation & purification; D008460:Meat; D008461:Meat Products; D011200:Poultry; D012756:Sheep; D012758:Shellfish; D013552:Swine
D003470:Culture Media