title: Detection of anti-HIV immunoglobulin M by particle agglutination following acute HIV infection.
abstract: In a study of 23 subjects infected with HIV, a modified particle agglutination assay was used to detect anti-HIV-specific immunoglobulin M (IgM). The presence of anti-HIV IgM was demonstrated in every subject, becoming detectable 1-2 weeks after the onset of acute symptoms, and showing a variable duration of 1-5 weeks. Anti-HIV immunoglobulin G (IgG) developed 1-2 weeks after anti-HIV IgM. Particle agglutination detected the presence of specific antibody up to 7-10 days earlier than the Abbott recombinant or Genetic Systems enzyme immunoassays. In this study, all subjects with acute infection became clearly positive by Western blot within 3 months of the onset of acute symptoms.
authors.affiliation: National HIV Reference Laboratory, Fairfield Hospital, Victoria, Australia.
authors.forename: D S
authors.initials: DS
authors.lastname: Healey
authors.forename: W J
authors.initials: WJ
authors.lastname: Maskill
authors.forename: I D
authors.initials: ID
authors.lastname: Gust
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology*; D000208:Acute Disease; D000372:Agglutination Tests; D000918:Antibody Specificity; D015153:Blotting, Western; D005260:Female; D006678:HIV / Q000276:immunology*; D015483:HIV Antibodies / Q000032:analysis*; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis*; D008297:Male; D013997:Time Factors
chemical_list: D015483:HIV Antibodies; D007075:Immunoglobulin M