Delayed c-fos proto-oncogene expression in the rat hippocampus induced by transient global cerebral ischemia: an in situ hybridization study.
The relative levels of c-fos mRNA in individual neurons of the hippocampal formation of rats is dramatically increased following 20 min of cerebral ischemia induced by 4-vessel occlusion. After 24 h of recirculation, a number of scattered neurons in the dentate hilus became hybridization positive. This effect appeared to peak between 24 and 48 h. A few neurons in the pyramidal cell layer of CA1 expressed c-fos as early as 24 h, but the most intense labeling in this region was seen at 72 h of recirculation. These results correlate well with the known distribution of delayed ischemic necrosis in the brain.
Jørgensen
M B
MB
Experimental Therapeutics Branch, NINDS, Bethesda, MD 20892.
Deckert
J
J
Wright
D C
DC
Gehlert
D R
DR
D000818:Animals; D005786:Gene Expression Regulation; D006624:Hippocampus / Q000378:metabolism* / Q000503:physiopathology; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D008297:Male; D009693:Nucleic Acid Hybridization; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger