title: Conformational change and aggregation of concanavalin A at high temperatures.
abstract: Increase of the beta sheet content and aggregation of concanavalin A (con A) induced at about 60 C were followed with circular dichroism (c.d.) and scattered light intensity (I90) on both metal-complexed and demetallized species. The conversion occurred at a higher temperature for metal-complexed species than for demetallized one. A concentration-independent conversion curve of metal-complexed species, obtained for a concentration range below around 6 microgram/ml (6 x 10(-3) kg m-3) with a midpoint at 57 degrees C, was well described in terms of a conformational equilibrium between two conformers. However, aggregation did exist even at a low concentration of 1 microgram/ml. Aggregation also occurred without the conformational change as found at the initial stage or in Tris buffer, which suggested the absence of direct coupling between the conformational change and the aggregation. Changes of c.d. at 222 nm, expected to represent the main chain conformation, differed from those at 290 nm reflecting the environment of side chain chromophores. Time courses of three properties examined, c.d. at 222 nm, at 290 nm, and I90, always exhibited a lag in the case of metal-complexed species while the lag was not observed in the case of demetallized species, however. Lag became longer in c.d. but it became shorter in I90 as the protein concentration increased.
authors.affiliation: Department of Chemistry, Faculty of Science, Nagoya University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Maeda
authors.forename: H
authors.initials: H
authors.lastname: Hattori
authors.forename: H
authors.initials: H
authors.lastname: Kanoh
mesh_terms: D002942:Circular Dichroism; D003208:Concanavalin A / Q000737:chemistry*; D006358:Hot Temperature; D007700:Kinetics; D008027:Light; D008670:Metals / Q000737:chemistry; D011487:Protein Conformation; D011489:Protein Denaturation; D012542:Scattering, Radiation
chemical_list: D008670:Metals; D003208:Concanavalin A