title: Purification and properties of an endo-1,4-beta-glucanase from Clostridium josui.
abstract: An enzyme active against carboxymethyl cellulose (CMC) was purified from the stationary-phase-culture supernatant of Clostridium josui grown in a medium containing ball-milled cellulose. The purification in the presence of 6 M urea yielded homogeneous enzyme after an approximately 50-fold increase in specific activity and a 13% yield. The enzyme had a molecular mass of 45 kilodaltons. The optimal temperature and pH of the enzyme against CMC were 60 degrees C and 6.8, respectively. The enzyme hydrolyzed cellotetraose, cellopentaose, and cellohexaose to cellobiose and cellotriose but did not hydrolyze cellobiose or cellotriose. A microcrystalline cellulose, Avicel, was also hydrolyzed significantly, but the extent of hydrolysis was remarkably less than that of CMC. On the basis of these results, the enzyme purified here is one of the endo-1,4-beta-glucanases. The N-terminal amino acid sequence of the enzyme is Tyr-Asp-Ala-Ser-Leu-Lys-Pro-Asn-Leu-Gln-Ile-Pro-Gln-Lys-Asn-Ile-Pro-Asn- Asn-Asp-Ala-Val-Asn-Ile-Lys.
authors.affiliation: Research and Technological Division, Nagoya Seiraku Co. Ltd., Japan.
authors.forename: T
authors.initials: T
authors.lastname: Fujino
authors.forename: J
authors.initials: J
authors.lastname: Sukhumavasi
authors.forename: T
authors.initials: T
authors.lastname: Sasaki
authors.forename: K
authors.initials: K
authors.lastname: Ohmiya
authors.forename: S
authors.initials: S
authors.lastname: Shimizu
mesh_terms: D000595:Amino Acid Sequence; D003013:Clostridium / Q000201:enzymology* / Q000254:growth & development; D043364:Endo-1,4-beta Xylanases; D006026:Glycoside Hydrolases / Q000302:isolation & purification*; D006868:Hydrolysis; D007700:Kinetics; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000378:metabolism; D014995:Xylosidases / Q000302:isolation & purification* / Q000378:metabolism / Q000502:physiology
chemical_list: D009844:Oligosaccharides; D006026:Glycoside Hydrolases; D014995:Xylosidases; D043364:Endo-1,4-beta Xylanases