title: Characteristics of interferon induced tryptophan metabolism in human cells in vitro.
abstract: Interferon-gamma-induced tryptophan metabolism of human macrophages was compared to ten human neoplastic cell lines of various tissue origin and to normal dermal human fibroblasts. Tryptophan and metabolites were determined in supernatants of cultures, after incubation for 48 h, by high-performance liquid chromatography with ultraviolet and fluorescence detection. With the exception of two cell lines (Hep G 2, hepatoma and CaCo 2, colon adenocarcinoma) in all of the ten other cells and cell lines tryptophan degradation was induced by interferon-gamma. Five of these ten formed only kynurenine (SK-N-SH, neuroblastoma; T 24, J 82, bladder carcinoma; A 431, epidermoid carcinoma; normal dermal fibroblasts), three formed kynurenine and anthranilic acid (U 138 MG, glioblastoma; SK-HEP-1, hepatoma; A 549, lung carcinoma). Only one line, A 498 (kidney carcinoma) showed the same pattern of metabolites as macrophages (kynurenine, anthranilic acid and 3-hydroxyanthranilic acid). Interferon-gamma regulated only the activity of indoleamine 2,3-dioxygenase. All other enzyme activities detected were independent of interferon-gamma, as shown by the capacity of the cells to metabolize L-kynurenine or N-formyl-L-kynurenine. Increasing the extracellular L-tryptophan concentration resulted in a marked induction of tryptophan degradation by macrophages. Contrarily, a significant decrease of the tryptophan degrading activity was observed when the extracellular L-tryptophan concentration was increased 2-fold with SK-N-SH, T 24 and J 82, 4-fold with A 431 and A 549 and 10-fold with U 138 MG and SK-HEP-1. The activity was unaffected by extracellular L-tryptophan with dermal fibroblasts and A 498. Though interferon-gamma was the most potent inducer of tryptophan metabolism, interferon-alpha and/or -beta showed small but distinct action on some of the cells. In all cells which reacted to interferon-gamma by enhanced expression of class I and/or class II major histocompatibility complex antigens tryptophan degradation was also inducible. These results demonstrate that induction of indoleamine 2,3-dioxygenase is a common feature of interferon-gamma action, that the extent of this induction is influenced by extracellular L-tryptophan concentrations and that indoleamine 2,3-dioxygenase is the only enzyme in the formation of 3-hydroxyanthranilic acid from tryptophan which is regulated by interferon-gamma.
authors.affiliation: Institute for Medical Chemistry and Biochemistry, University of Innsbruck, Austria.
authors.forename: G
authors.initials: G
authors.lastname: Werner-Felmayer
authors.forename: E R
authors.initials: ER
authors.lastname: Werner
authors.forename: D
authors.initials: D
authors.lastname: Fuchs
authors.forename: A
authors.initials: A
authors.lastname: Hausen
authors.forename: G
authors.initials: G
authors.lastname: Reibnegger
authors.forename: H
authors.initials: H
authors.lastname: Wachter
mesh_terms: D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D004790:Enzyme Induction; D006680:HLA Antigens / Q000096:biosynthesis; D006684:HLA-DR Antigens / Q000096:biosynthesis; D006801:Humans; D007371:Interferon-gamma / Q000502:physiology*; D007737:Kynurenine / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D010105:Oxygenases / Q000096:biosynthesis; D014364:Tryptophan / Q000378:metabolism*; D014366:Tryptophan Oxygenase; D014407:Tumor Cells, Cultured
chemical_list: D006680:HLA Antigens; D006684:HLA-DR Antigens; D007737:Kynurenine; D007371:Interferon-gamma; D014364:Tryptophan; D010105:Oxygenases; D014366:Tryptophan Oxygenase