Quantification of apolipoprotein A-1 in cow serum by single radial immunodiffusion.
A single radial immunodiffusion assay was developed to determine the concentration of the major apolipoprotein A-I in bovine serum. Assay was performed using agarose gel plates with monospecific rabbit antiserum. Standards made with bovine high density lipoproteins or serum 10 times diluted were applied in 3-microliters amounts to each well in the gel. The assay working range was 2 to 22 mg of apolipoprotein A-I. The intraassay and interassay CV were 3.7 and 4.9%, respectively. Using protein A-I. The intraassay and interassay CY were 3.7 and 4.9%, respectively. Using radial immunodiffusion assay, 23 serum samples from cows at various stages of lactation were analyzed. Apolipoprotein A-I was significantly increased in midlactation compared with concentrations in early lactation or dry period.
Mazur
A
A
Laboratoire des Maladies Métaboliques, INRA, Theix, Ceyrat.
Marcos
E
E
Cardot
P
P
Ayrault-Jarrier
M
M
Rayssiguier
Y
Y
D000818:Animals; D001054:Apolipoproteins A / Q000097:blood*; D002417:Cattle / Q000097:blood*; D005260:Female; D005779:Immunodiffusion / Q000662:veterinary; D007122:Immunoelectrophoresis
D001054:Apolipoproteins A