Expression of cytokine mRNA in leukemic cells from adult T cell leukemia patients.
HTLV-I infection of peripheral mature T cells appears to induce the expression of cellular genes including those of some cytokines and their receptors. We examined the expression of interleukin-1 alpha (IL-1 alpha), IL-1 beta, IL-2, IL-3, IL-4 and granulocyte/macrophage colony-stimulating factor (GM-CSF) at the mRNA level in fresh leukemic cells from 20 adult T cell leukemia patients to see whether there is any association between cytokine expression and HTLV-I expression and between their expression and clinical manifestations such as hypercalcemia or neutrophilia. IL-1 alpha, IL-1 beta and IL-3 expression was observed in 3, 7 and 1 of 20 cases examined, respectively. However, there seemed to be no association between IL-1 expression and clinical manifestations. IL-2, IL-4 and GM-CSF mRNA expression was not detected. HTLV-I viral RNA expression was detected only in one case in which IL-3 mRNA was expressed in both peripheral blood and lymph node cells and a relatively high proportion of leukemic cells expressed IL-2 receptor (p55, Tac). Thus, in the present study we could not find any correlation between cytokine expression and HTLV-I expression in peripheral blood fresh leukemic cells except in one unusual case.
Kodaka
T
T
First Division of Internal Medicine, Faculty of Medicine, Kyoto University.
Uchiyama
T
T
Umadome
H
H
Uchino
H
H
D000328:Adult; D000368:Aged; D003115:Colony-Stimulating Factors / Q000235:genetics*; D015342:DNA Probes; D005260:Female; D005786:Gene Expression Regulation; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000235:genetics*; D015368:Human T-lymphotropic virus 1 / Q000235:genetics; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics; D007376:Interleukin-2 / Q000235:genetics; D007377:Interleukin-3 / Q000235:genetics; D015847:Interleukin-4; D007378:Interleukins / Q000235:genetics*; D015458:Leukemia, T-Cell / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics
D003115:Colony-Stimulating Factors; D015342:DNA Probes; D006133:Growth Substances; D007375:Interleukin-1; D007376:Interleukin-2; D007377:Interleukin-3; D007378:Interleukins; D012333:RNA, Messenger; D012367:RNA, Viral; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor