Cholesterol and apolipoprotein distribution in plasma high-density-lipoprotein subclasses from zinc-deficient rats.
Effect of zinc deficiency on the composition of plasma high-density-lipoprotein (HDL) subclasses was investigated in adult male rats (ZD) fed a diet containing 2.8 micrograms of Zn/g as compared with pair-fed (PF) and ad libitum-fed (CT) controls given 30.8 micrograms of Zn/g. Three HDL subclasses were isolated by heparin-affinity chromatography: HDL containing no apo E (HDL-E0), HDL with a moderate amount of apo E (HDL-E1), and HDL highly enriched in apo E (HDL-E2). The plasma levels of total HDL cholesterol and protein were markedly decreased in ZD. The decreases were due primarily to a significant decline in circulating HDL-E0 particles. The percent content of free cholesterol in HDL-E2 was increased in ZD compared with PF and CT. Zn deficiency decreased the plasma concentrations of apo A-I and apo C of HDL-E0 and total apo E associated with HDL-E1 and HDL-E2. The mechanisms for such changes await further investigation.
Koo
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Department of Biochemistry, School of Medicine, Oral Roberts University, Tulsa, OK 74171.
Lee
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D000818:Animals; D016632:Apolipoprotein A-I; D001053:Apolipoproteins / Q000097:blood*; D001054:Apolipoproteins A / Q000097:blood; D001056:Apolipoproteins C / Q000097:blood; D001057:Apolipoproteins E / Q000097:blood; D001835:Body Weight; D002784:Cholesterol / Q000097:blood*; D004032:Diet; D004435:Eating; D008075:Lipoproteins, HDL / Q000097:blood* / Q000302:isolation & purification; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D015032:Zinc / Q000097:blood / Q000172:deficiency*
D016632:Apolipoprotein A-I; D001053:Apolipoproteins; D001054:Apolipoproteins A; D001056:Apolipoproteins C; D001057:Apolipoproteins E; D008075:Lipoproteins, HDL; D002784:Cholesterol; D015032:Zinc