Stability of canine factor VIII: coagulant activity in vitro.
A pilot study was undertaken to assess the stability of canine factor VIII:coagulant (FVIII:C) activity over three days, under various storage conditions (plasma at 4, 20 and 37 degrees C, whole blood at 4 and 20 degrees C). Blood collected from normal and hemophiliac dogs was used. Both plasma and whole blood samples appeared to be stable for up to 48 h at 4 and 20 degrees C. A subsequent study evaluated FVIII:C stability at 4 and 20 degrees C when stored as whole blood only. Samples were tested at 0, 24 and 48 h after collection. At 4 degree C there was a significant decline at 24 h (p less than 0.05), from 110% to 97% (mean values). Although the mean value was further decreased at 48 h (89%) this was not significant (p greater than 0.05). No significant change in FVIII:C activity was observed in whole blood stored at 20 degrees C for 24 or 48 h (110% and 107% respectively). These results suggest that canine whole blood samples collected into sodium citrate stored at 20 degrees C are adequate for routine FVIII:C assay for up to 48 h after collection.
Mansell
P D
PD
Department of Veterinary Paraclinical Sciences, University of Melbourne, Werribee, Victoria, Australia.
Parry
B W
BW
D000818:Animals; D004283:Dog Diseases / Q000097:blood*; D004285:Dogs / Q000097:blood*; D005169:Factor VIII / Q000502:physiology*; D006467:Hemophilia A / Q000097:blood / Q000662:veterinary*; D066298:In Vitro Techniques; D008297:Male; D013048:Specimen Handling / Q000662:veterinary; D013696:Temperature
D005169:Factor VIII