[Improvement of the microbiological diagnosis of tuberculosis with an account of the detection of L forms and ultrasmall mycobacteria].
Possible addition of various drugs to the Dorozhkova nutrient medium for isolation of L-forms of M. tuberculosis was studied with a purpose of preventing microbial contamination of the cultures. It was shown that the use of malachite green as 1% sterile aqueous solution in an amount of 11 ml per 1 liter of the medium lowered the number of the sprouts from 35 to 4.5 per cent. Various microscopic methods were tested to choose the most suitable one for isolating L-forms of M. tuberculosis. Different nutrient media for additional cultivation and isolation of revertants from ultrasmall mycobacteria were compared. The Dorozhkova medium provided reversion of the ultrasmall mycobacterial L-forms in 12 per cent of the cases. The respective index on the routine Sauton and Shkolnikova media amounted to 5 per cent.
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D001431:Bacteriological Techniques; D003470:Culture Media; D006801:Humans; D007740:L Forms / Q000302:isolation & purification*; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D014376:Tuberculosis / Q000175:diagnosis*
D003470:Culture Media
Sovershenstvvanie mikrobiologicheskoĭ diagnostiki tuberkuleza s uchetom vyiavleniia L-form i ul'tramelkikh mikobakteriĭ.