Duodenal infusion of fat, cholecystokinin secretion and satiety in the pig.
The influence of the cholecystokinin (CCK) antagonist L-364,718 (0.1 mg/kg) on short-term control of food intake was studied in 6 pigs. Arterial injection of L-364,718 abolished the inhibition of intake to CCK octapeptide infusion (4 micrograms/kg/hr; from 42% p less than 0.001, to 97% of control intake), but did not alter control intake (99%). Injection of L-364,718 also abolished the inhibition of intake to duodenal infusion of emulsified fat (12 g/hr; from 76% p less than 0.001 to 105%) and of monoglyceride (24 g/hr; from 64% p less than 0.001 to 101%), but did not alter the inhibition to oleic acid (60 g/hr; 48% p less than 0.01 and 61% p less than 0.02), to glycerol (127 g/hr; 84% p less than 0.05 and 89%) or to glucose (144 g/hr; 78% p less than 0.02 and 69% p less than 0.001). These results suggest that monoglyceride-induced CCK secretion is mainly responsible for the satiety to duodenal fat in the pig, but that there is also a CCK-independent effect via the fatty acid. The results further indicate that intake of a normal barley-based diet (2% fat) is controlled via CCK-independent mechanisms.
Gregory
P C
PC
Rowett Research Institute, Bucksburn, Aberdeen.
McFadyen
M
M
Rayner
D V
DV
D000818:Animals; D001570:Benzodiazepinones / Q000494:pharmacology*; D002766:Cholecystokinin / Q000037:antagonists & inhibitors / Q000378:metabolism* / Q000502:physiology; D020109:Devazepide; D004305:Dose-Response Relationship, Drug; D004386:Duodenum; D004435:Eating / Q000187:drug effects; D005217:Fat Emulsions, Intravenous / Q000008:administration & dosage / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology; D005989:Glycerides / Q000494:pharmacology; D005990:Glycerol / Q000494:pharmacology; D009829:Oleic Acids / Q000494:pharmacology; D010288:Parenteral Nutrition; D012527:Satiation / Q000187:drug effects*; D012844:Sincalide / Q000494:pharmacology*; D013552:Swine
D001570:Benzodiazepinones; D005217:Fat Emulsions, Intravenous; D005989:Glycerides; D009829:Oleic Acids; D002766:Cholecystokinin; D005947:Glucose; D020109:Devazepide; D012844:Sincalide; D005990:Glycerol