[The effect of dimethylsulfoxide and thiourea on the water diffusion permeability of the E. coli membrane].
It is shown that diffusive water permeability of E. coli cell membranes at 4-24 degrees C is in the range from 16.6 to 35.0 microM.s-1, that is close to erythrocyte membrane water permeability, but higher than that of lipid bilayers. Cryoprotectants (DMSO and thiourea) at the concentration of 1.0 M considerably decrease water bacterial membrane permeability. 1.5- and 2-fold, respectively. The obtained results are discussed in relation to two possible water transport ways through pores of the protein nature or lipid bilayer damages.
Sakharov
B V
BV
Volkov
V Ia
VIa
D001692:Biological Transport; D002463:Cell Membrane Permeability / Q000187:drug effects*; D004058:Diffusion; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism; D008051:Lipid Bilayers / Q000378:metabolism; D013890:Thiourea / Q000494:pharmacology*; D014867:Water / Q000378:metabolism*
D008051:Lipid Bilayers; D014867:Water; D013890:Thiourea; D004121:Dimethyl Sulfoxide
Vliianie dimetilsul'foksida i tiomocheviny na diffuzionnuiu vodnuiu pronitsaemost' obolochek E. coli.