Gamma-interferon induces prostacyclin and prostaglandin but not lipoxygenase product synthesis in rat endothelial cells.
The major arachidonic acid metabolites excreted by untreated rat heart endothelial cells were prostacyclin (PGI2), 5-hydroxyeicosatetraenoic acid (5-HETE) and 15-hydroxyeicosatetraenoic acid (15-HETE). Trace amounts of both prostaglandin E (PGE) and thromboxane B2 (TXB2) were also produced. The activity of leukotriene B4 (LTB4) was the same as baseline activity. Gamma-interferon (gamma-IFN) treatment increased the production of PGI2 and PGE more than three-fold compared to control values. Thromboxane production increased two-fold while the excretion of 5-HETE and 15-HETE was unaffected. No LTB4 excretion was seen after gamma-IFN stimulus. Methylprednisolone downregulated the 5-HETE, 15-HETE and prostacyclin production both in normal and gamma-IFN treated cells. Indomethacin decreased prostacyclin excretion in untreated cells and also decreased gamma-IFN-induced prostacyclin and PGE excretion. Taken together these results indicate that rat microvascular endothelial cells excrete prostaglandins and prostacyclin, but not leukotrienes.
Mattila
P
P
Transplantation Laboratory, University of Helsinki, Finland.
Renkonen
R
R
D000818:Animals; D002478:Cells, Cultured; D002855:Chromatography, Thin Layer; D004537:Eicosanoic Acids / Q000302:isolation & purification; D004730:Endothelium, Vascular / Q000378:metabolism*; D011464:Epoprostenol / Q000096:biosynthesis*; D007213:Indomethacin / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology*; D008084:Lipoxygenase / Q000096:biosynthesis*; D008775:Methylprednisolone / Q000494:pharmacology; D009206:Myocardium / Q000378:metabolism; D010640:Phenothiazines / Q000494:pharmacology; D011458:Prostaglandins E / Q000096:biosynthesis*; D051381:Rats; D013929:Thromboxane B2 / Q000096:biosynthesis
D004537:Eicosanoic Acids; D010640:Phenothiazines; D011458:Prostaglandins E; D013929:Thromboxane B2; D007371:Interferon-gamma; C053726:4-bromo-2,7-dimethoxy-3H-phenothiazin-3-one; D011464:Epoprostenol; D008084:Lipoxygenase; D008775:Methylprednisolone; D007213:Indomethacin