ATP stimulates secretion in human neutrophils and HL60 cells via a pertussis toxin-sensitive guanine nucleotide-binding protein coupled to phospholipase C.
Human neutrophils and HL60 cells respond to extracellular ATP by causing exocytotic secretion. Secretion is accompanied by increases in inositol phosphates and a rise in cytosol Ca2+. The responses to ATP are blocked by pertussis toxin pretreatment, indicating the involvement of a guanine nucleotide regulatory protein. Other nucleotides that are active in promoting secretion are ATP gamma S, UTP, ITP and AppNHp, whilst 8-bromo-ATP, AppCH2p, ADP, AMP and adenosine are inactive.
Cockcroft
S
S
Department of Experimental Pathology, School of Medicine, University College London, England.
Stutchfield
J
J
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology*; D000266:Adenylyl Imidodiphosphate / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D005089:Exocytosis / Q000187:drug effects*; D019204:GTP-Binding Proteins / Q000502:physiology*; D005966:Glucuronidase / Q000378:metabolism; D006801:Humans; D007293:Inosine Triphosphate / Q000494:pharmacology; D007295:Inositol Phosphates / Q000378:metabolism; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D037342:Pertussis Toxin; D014407:Tumor Cells, Cultured; D010738:Type C Phospholipases / Q000378:metabolism*; D014544:Uridine Triphosphate / Q000494:pharmacology; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
D007295:Inositol Phosphates; D010566:Virulence Factors, Bordetella; D007293:Inosine Triphosphate; D000266:Adenylyl Imidodiphosphate; C022571:adenosine 5'-O-(3-thiotriphosphate); D009240:N-Formylmethionine Leucyl-Phenylalanine; D000255:Adenosine Triphosphate; D037342:Pertussis Toxin; D010738:Type C Phospholipases; D005966:Glucuronidase; D019204:GTP-Binding Proteins; D002118:Calcium; D014544:Uridine Triphosphate