title: [Localization of a lysine residue near the site of initiating substrate binding of T7 bacteriophage RNA polymerase].
abstract: A highly selective affinity label was introduced into the T7 phage RNA polymerase by means of GMP ortho-formylphenyl ester and [alpha-32P]UTP nearby the enzyme's active site, which was located using limited cleavage technique. Hydroxylamine, bromine, N-chlorosuccinimide, and cyanogen bromide were employed as the reagents. Analysis of gel-electrophoretic patterns of the cleavage products led to a conclusion that Lys631 is the target of labelling. The region nearby this residue has a high degree of sequence homology with regions of RNA polymerases from T3 and SP6 phages and yeast mitochondria.
authors.forename: T G
authors.initials: TG
authors.lastname: Maksimoa
authors.forename: A A
authors.initials: AA
authors.lastname: Mustaev
authors.forename: E F
authors.initials: EF
authors.lastname: Zaĭchikov
authors.forename: L V
authors.initials: LV
authors.lastname: Baranova
authors.forename: V P
authors.initials: VP
authors.lastname: Kumarev
mesh_terms: D000345:Affinity Labels; D001665:Binding Sites; D001966:Bromine; D003488:Cyanogen Bromide; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D006898:Hydroxylamines; D008239:Lysine / Q000032:analysis*; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D013388:Succinimides; D013604:T-Phages / Q000201:enzymology*
chemical_list: D000345:Affinity Labels; D006898:Hydroxylamines; D013388:Succinimides; C015752:N-chlorosuccinimide; D012321:DNA-Directed RNA Polymerases; D008239:Lysine; D003488:Cyanogen Bromide; D001966:Bromine
vernacular_title: Lokaliatsiia ostatka lizina v oblasti sviazyvaniia initsiruiushchego substrata RNK-polimerazy bakteriofaga T7.