title: Immune regulation of the L5178Y murine tumor-dormant state: induction of cell- and soluble factor-mediated inhibition of tumor cell growth by tumor necrosis factor and gamma-interferon.
abstract: Small concentrations of recombinant murine tumor necrosis factor (rMuTNF) synergized with recombinant murine gamma-interferon (rMuIFN-gamma) to inhibit tumor cell growth in peritoneal cell (PC) cultures from tumor-dormant mice. A soluble inhibitor of tumor cell growth was produced in the rMuTNF-treated PC cultures, but there was no synergistic enhancement of production of this inhibitor by the addition of rMuIFN-gamma. Treatment of both plastic-adherent PC and nonadherent PC cultures which contained L5178Y cells with rMuTNF + rMuIFN-gamma resulted in inhibition of tumor cell growth. However, in the absence of L5178Y cells, rMuTNF + rMuIFN-gamma induced antitumor cytotoxic activity in the plastic-adherent but not in the plastic nonadherent PC. These results indicate that the antitumor activity of rMuTNF + rMuIFN-gamma in PC from tumor-dormant mice involves both cell- and soluble factor-mediated mechanisms.
authors.affiliation: Department of Pathology and Laboratory Medicine, Hahnemann University, Philadelphia, Pennsylvania 19102.
authors.forename: Y
authors.initials: Y
authors.lastname: Suzuki
authors.forename: L P
authors.initials: LP
authors.lastname: Chen
authors.forename: C M
authors.initials: CM
authors.lastname: Liu
authors.forename: T
authors.initials: T
authors.lastname: Morita
authors.forename: E F
authors.initials: EF
authors.lastname: Wheelock
mesh_terms: D000818:Animals; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D004357:Drug Synergism; D005260:Female; D007371:Interferon-gamma / Q000494:pharmacology*; D051379:Mice; D008811:Mice, Inbred DBA; D009374:Neoplasms, Experimental / Q000276:immunology* / Q000473:pathology; D010529:Peritoneal Cavity / Q000276:immunology; D011994:Recombinant Proteins / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma