[Low sensitivity of choriocarcinoma cell lines to tumor necrosis factor (TNF)-alpha].
Sensitivity of cultured choriocarcinoma cell lines to tumor necrosis factor (TNF)-alpha and interferon (IFN)-gamma was examined and compared with that of other cultured tumor cell lines. Tumor cells were cultured with 1,000 mu/ml of TNF-alpha and/or 100 mu/ml of IFN-gamma for 24hr. Antitumor effects of the lymphokines were measured by 3H-thymidine uptake and tetrazolium salt (MTT) colorimetric assay. The results revealed that TNF-alpha and/or IFN-gamma had little anti-tumor effect on the choriocarcinoma cell lines tested, while they had cytostatic and cytotoxic effects on other cultured tumor cell lines including Panc-1 (pancreatic carcinoma cell line), Lovo (colon carcinoma cell line) and Ishikawa (uterine endometrial carcinoma cell line). We also examined the effect of TNF-alpha and IFN-gamma on the expression of major histocompatibility complex (MHC) class I antigens in these tumor cell lines by means of cellular binding radioimmunoassay. No enhancing effect was observed on choriocarcinoma cell lines after the treatment with TNF-alpha and/or IFN-gamma. These results suggested the existence of a unique property of choriocarcinoma cells which results in the resistance to host immune attacks.
Kameda
T
T
Department of Obstetrics and Gynecology, Osaka University Medical School.
Negoro
T
T
Samejima
Y
Y
Takahashi
S
S
Koyama
M
M
Matsuzaki
N
N
Saji
F
F
Tanizawa
O
O
D002822:Choriocarcinoma / Q000276:immunology / Q000473:pathology*; D004338:Drug Combinations; D004351:Drug Resistance; D015395:Histocompatibility Antigens Class I / Q000032:analysis; D006801:Humans; D007371:Interferon-gamma / Q000008:administration & dosage / Q000494:pharmacology; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D013778:Tetrazolium Salts; D014407:Tumor Cells, Cultured / Q000276:immunology / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000008:administration & dosage / Q000494:pharmacology*
D004338:Drug Combinations; D015395:Histocompatibility Antigens Class I; D011994:Recombinant Proteins; D013778:Tetrazolium Salts; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma