Covalent modification of proteins in Bacillus subtilis during the process of sporulation, germination and outgrowth.
Cells of Bacillus subtilis 168+ were labeled with 32P-orthophosphate during the process of sporulation, germination and outgrowth. By two-dimensional gel electrophoresis, at least 30 protein species were found to be radioactively labeled; 30% of these were modified by phosphorylation. Significant changes in the protein phosphorylation pattern during growth and cellular differentiation could be demonstrated. Using gamma-32P-ATP evidence for an ATP-dependent protein kinase was also obtained. Under these conditions 4 proteins with a molecular mass of 109,600; 103,100; 73,300 and 32,200 Da were found to be phosphorylated.
Köhler
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E
Institut für Mikrobiologie der Georg-August Universität Göttingen, F.R.G.
Antranikian
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G
D001412:Bacillus subtilis / Q000254:growth & development / Q000502:physiology*; D001426:Bacterial Proteins / Q000378:metabolism*; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D013171:Spores, Bacterial / Q000254:growth & development / Q000502:physiology
D001426:Bacterial Proteins; D011494:Protein Kinases