Amyloid enhancing factor (AEF) in the aging mouse.
Amyloid enhancing factor (AEF) activity was tested in spleen homogenates from 52, 28, and 9-week-old untreated mice. Strong AEF activity was present in the supernatant of spleen homogenate from the 52-week-old mice. Only weak activity was observed in the supernatant from the 28-week-old mice, and none was seen in that from the 9-week-old mice. Crude spleen homogenates from the 52-week-old mice were subjected to Sephacryl S-300 gel filtration. AEF activity existed in the first gel filtration peak. These findings suggest that AEF increases in the spleens of aged mice and that AEF may be a substance of high molecular weight.
Yokota
T
T
First Department of Pathology, Yamaguchi University School of Medicine, Japan.
Ishihara
T
T
Kawano
H
H
Takahashi
M
M
Fujinaga
Y
Y
Uchino
F
F
D000375:Aging / Q000378:metabolism*; D000818:Animals; D002850:Chromatography, Gel; D006023:Glycoproteins / Q000378:metabolism*; D007150:Immunohistochemistry; D051379:Mice; D008815:Mice, Inbred Strains / Q000378:metabolism*; D013154:Spleen / Q000166:cytology / Q000378:metabolism
D006023:Glycoproteins; C046748:amyloid enhancing factor