title: Phosphorus-31 nuclear magnetic resonance spectroscopy of toad retina.
abstract: Phosphorus-31 nuclear magnetic resonance (31P-NMR) spectra were obtained from living toad retinae and toad retinal extracts at 4 degrees C. Several phosphorus metabolites--nucleoside di- and triphosphates (NTP), phosphocreatine, phosphodiesters, inorganic phosphate, and phosphomonoesters--were identified from the spectra of whole retinae. The intracellular pH was determined to be 7.27 +/- 0.06 at 4 degrees C and the intracellular MgNTP/NTP ratio was at least 0.77. These results are consistent with those reported by other techniques, and they show that 31P-NMR spectroscopy can be used for noninvasively and quantitatively studying the metabolism of living toad retinae, and for monitoring its changes over time.
authors.affiliation: Department of Physiology and Biophysics, University of Illinois, Urbana 61801.
authors.forename: D V
authors.initials: DV
authors.lastname: Apte
authors.forename: Y
authors.initials: Y
authors.lastname: Koutalos
authors.forename: D K
authors.initials: DK
authors.lastname: McFarlane
authors.forename: M J
authors.initials: MJ
authors.lastname: Dawson
authors.forename: T G
authors.initials: TG
authors.lastname: Ebrey
mesh_terms: D000818:Animals; D002024:Bufo marinus; D005612:Freeze Drying; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000032:analysis / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D010759:Phosphorus Isotopes; D012160:Retina / Q000032:analysis*
chemical_list: D010759:Phosphorus Isotopes; D008274:Magnesium