[Insulin-phospholipid interactions. A study in monolayers by measuring surface potential].
Surface potential (delta V) measurements were performed to assess information on insulin penetration/interaction with dipalmitoylphosphatidylcholine (DPPC) monolayers spread at the water-air interface. The results reveal existence of the threshold surface density value of spread lipid molecules (7.5 x 10(13) molecules/cm2) above which none penetration of insulin molecules occurs. Surface potential data clearly indicate also that insulin penetration/interaction with DPPC monolayers is enhanced in the presence of the second studied constituent of these monolayers in the order DPPC + stearylamine greater than DPPC + cholesteryl betainate greater than DPPC + cholesterol greater than DPPC. The results clearly indicate the existence of two types of phospholipid-insulin interactions namely: adsorption and penetration.
Weingarten
C
C
Physico-Chimie des Surfaces et Innovation en Pharmacotechnie, U.R.A.-C.N.R.S., Université Paris-Sud.
Baszkin
A
A
Puisieux
F
F
Moufti
A
A
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000493:pharmacokinetics; D000200:Action Potentials; D000327:Adsorption; D000588:Amines / Q000493:pharmacokinetics; D001693:Biological Transport, Active; D002784:Cholesterol / Q000493:pharmacokinetics; D004347:Drug Interactions; D007328:Insulin / Q000378:metabolism* / Q000493:pharmacokinetics; D014677:Pharmaceutical Vehicles; D010743:Phospholipids / Q000378:metabolism* / Q000493:pharmacokinetics
D000588:Amines; D007328:Insulin; D014677:Pharmaceutical Vehicles; D010743:Phospholipids; D015060:1,2-Dipalmitoylphosphatidylcholine; D002784:Cholesterol; C009317:stearylamine
Interactions phospholipides-insuline. Etude en couches monomoléculaires par des mesures de potentiel de surface.