Detection of calcitonin and CGRP mRNAs in thyroid parafollicular cells using biotinylated probes.
Probes for hybridocytochemistry were produced using SP6 RNA polymerase system and biotinylated 11-UTP as a label. Probes were obtained capable of detecting calcitonin-mRNA and CGRP-mRNA. Probe quality was tested using Southern blotting. Hybridocytochemistry on rat thyroid sections demonstrated both mRNAs in all parafollicular cells.
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Department of Histology, Medical Academy, Poznań, Poland.
Schäfer
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D000818:Animals; D002116:Calcitonin / Q000235:genetics*; D015740:Calcitonin Gene-Related Peptide; D005786:Gene Expression Regulation; D009479:Neuropeptides / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D015347:RNA Probes; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D013961:Thyroid Gland / Q000033:anatomy & histology*
D009479:Neuropeptides; D015347:RNA Probes; D012333:RNA, Messenger; D002116:Calcitonin; D015740:Calcitonin Gene-Related Peptide