title: Human nuclear NAD+ ADP-ribosyltransferase(polymerizing): organization of the gene.
abstract: Human nuclear NAD+: protein ADP-ribosyltransferase(polymerizing) [pADPRT; poly(ADP-ribose)poly-merase; EC 2.4.2.30] is a DNA-dependent protein-modifying enzyme composed of several domains important for DNA binding, automodification, and NAD binding. We report that the human pADPRT gene is 43 kb in length and is split into 23 exons. All the intron-exon boundaries correspond to a canonical splice consensus sequence. Each of the four metal coordinating sites putatively forming the two zinc fingers of the DNA-binding domain is encoded separately. The automodification domain and the NAD-binding domain are coded for by 4 and 12 exons, respectively.
authors.affiliation: Institut für Biochemie, Universität Innsbruck, Austria.
authors.forename: B
authors.initials: B
authors.lastname: Auer
authors.forename: U
authors.initials: U
authors.lastname: Nagl
authors.forename: H
authors.initials: H
authors.lastname: Herzog
authors.forename: R
authors.initials: R
authors.lastname: Schneider
authors.forename: M
authors.initials: M
authors.lastname: Schweiger
mesh_terms: D036002:ADP Ribose Transferases / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D003360:Cosmids / Q000235:genetics; D005091:Exons; D005796:Genes; D006801:Humans; D066298:In Vitro Techniques; D007438:Introns; D008969:Molecular Sequence Data; D011065:Poly(ADP-ribose) Polymerases; D015183:Restriction Mapping
chemical_list: D036002:ADP Ribose Transferases; D011065:Poly(ADP-ribose) Polymerases