Biosynthesis of caldariellaquinone in Sulfolobus spp.
The biosynthesis of caldariellaquionone (CQ) was studied in species of Sulfolobus by measuring the incorporation of stable isotopically labeled tyrosines into CQ. By feeding a series of tyrosines labeled with deuterium or 13C and then measuring the extent and position at which label was incorporated into CQ by mass spectrometry, it was shown that more than 95% of the label was incorporated into the benzo[b]thiophen-4,7-quinone moiety of CQ. From the labeling experiments, it is concluded that the benzo[b]thiophen-4,7-quinone is derived as an intact unit from all of the carbons of tyrosine except C-1.
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Department of Biochemistry and Nutrition, Virginia Polytechnic Institute and State University, Blacksburg 24061.
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D002247:Carbon Isotopes; D003903:Deuterium; D006091:Gram-Negative Chemolithotrophic Bacteria / Q000254:growth & development / Q000378:metabolism*; D007553:Isotope Labeling; D013058:Mass Spectrometry; D010103:Oxygen Isotopes; D011809:Quinones / Q000302:isolation & purification / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism
D002247:Carbon Isotopes; D010103:Oxygen Isotopes; D011809:Quinones; D014443:Tyrosine; C014380:caldariellaquinone; D003903:Deuterium