Lymphocyte subset counting by immunoperoxidase using an automated routine hematologic analyzer.
Using monoclonal antibodies against CD2, CD4, CD8 and CD19 antigens and an automated biotin-avidin immunoperoxidase technique on whole blood samples, we evaluated the technical performance and clinical usefulness of lymphocyte subset counting by the routine hematology analyzer Technicon H*1. Statistical evaluation demonstrated excellent precision and very good correlation with the immunofluorimetric flow cytometer Ortho Spectrum III. Correlation between manual immunofluorescence at the microscope and the H*1 method was much poorer, owing to the high intrinsic imprecision of the manual method. Reference ranges obtained with the H*1 immunoperoxidase method in 44 healthy subjects closely matched those obtained with the Spectrum III. In 46 subjects with or at risk for HIV infection, we found with the H*1 method a significant decrease in CD4+ cells and in the CD4+/CD8+ cell ratio, which was progressively more marked in HIV- negative patients with lymphadenopathic syndrome, AIDS-related complex, and in patients with full-blown AIDS.
d'Onofrio
G
G
Menichella
G
G
Zini
G
G
Rumi
C
C
Pierelli
L
L
Quarantelli
M
M
Puggioni
P L
PL
Mango
G
G
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000032:analysis; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006679:HIV Seropositivity / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation*; D007958:Leukocyte Count / Q000295:instrumentation*; D008214:Lymphocytes / Q000145:classification* / Q000201:enzymology; D011237:Predictive Value of Tests
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation