title: Reduced growth hormone releasing factor (GHRF)-like immunoreactivity and GHRF gene expression in the hypothalamus of aged rats.
abstract: Growth hormone releasing factor-like immunoreactivity (GHRF-LI) and GHRF mRNA levels were evaluated in the hypothalamus of aged (24 months) and young (3 months) rats by semiquantitative immunocytochemistry and slot blot hybridization technique, respectively. Simultaneous detection of reduced GHRF-LI and GHRF mRNA levels in aged rats as compared to young counterparts demonstrates the existence in aged rats of an impaired function of GHRF-producing neurons.
authors.affiliation: Department of Pharmacology, University of Milan, Italy.
authors.forename: V
authors.initials: V
authors.lastname: DeGennaro Colonna
authors.forename: M
authors.initials: M
authors.lastname: Zoli
authors.forename: D
authors.initials: D
authors.lastname: Cocchi
authors.forename: A
authors.initials: A
authors.lastname: Maggi
authors.forename: P
authors.initials: P
authors.lastname: Marrama
authors.forename: L F
authors.initials: LF
authors.lastname: Agnati
authors.forename: E E
authors.initials: EE
authors.lastname: Müller
mesh_terms: D000375:Aging; D000818:Animals; D005786:Gene Expression Regulation; D005796:Genes; D013007:Growth Hormone-Releasing Hormone / Q000032:analysis* / Q000235:genetics; D007031:Hypothalamus / Q000254:growth & development*; D008297:Male; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D013007:Growth Hormone-Releasing Hormone