title: [Effects of various factors on the growth and function of a human hepatoblastoma cell line, HuH-6--an approach for serum-free cell culture].
abstract: The effects on a human hepatoblastoma cell line (HuH-6) of serum and Ca2+ were investigated. A higher concentration of serum reduced the production of alpha-fetoprotein, whereas Ca2+ enhanced that of both albumin and alpha-fetoprotein. In view of these facts, a serum-free medium using RPMI-1640 supplemented with lactalbumin hydrolysate, epidermal growth factor, hydrocortisone, insulin, chrelatoxine, glucagone and selenium was then developed. Collagen type IV was superior to collagen type I, laminin, fibronectin and plastic in supporting the growth of HuH-6 in the serum-free medium. Higher amounts of both albumin and alpha-fetoprotein were secreted in HuH-6 cultured in serum-free medium than in serum-supplemented medium.
authors.affiliation: Third Department of Internal Medicine, Toyama Medical and Pharmaceutical University.
authors.forename: M
authors.initials: M
authors.lastname: Miyagiwa
authors.forename: T
authors.initials: T
authors.lastname: Ichida
authors.forename: H
authors.initials: H
authors.lastname: Sasaki
authors.forename: T
authors.initials: T
authors.lastname: Tokiwa
authors.forename: J
authors.initials: J
authors.lastname: Sato
mesh_terms: D001769:Blood; D002118:Calcium / Q000494:pharmacology; D003470:Culture Media; D016326:Extracellular Matrix Proteins / Q000494:pharmacology; D006394:Hemangiosarcoma / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D012709:Serum Albumin / Q000096:biosynthesis; D014407:Tumor Cells, Cultured; D000509:alpha-Fetoproteins / Q000096:biosynthesis
chemical_list: D003470:Culture Media; D016326:Extracellular Matrix Proteins; D012709:Serum Albumin; D000509:alpha-Fetoproteins; D002118:Calcium