title: Unambiguous typing for HLA-DQ TA10 and 2B3 specificities using specific oligonucleotide probes.
abstract: Oligonucleotide probes specific for the serologically defined TA10 and 2B3 specificities were selected based on a comparison of the available HLA-DQ beta sequences. Panel and family segregation studies confirm a complete correlation between the reactivities of the selected probes and the TA10/IIB3 antibodies. The Glu residue at position 45 of the HLA-DQ beta chain is specific for the TA10 determinants, and a DQ beta Gly-Val-Tyr sequence is found at position 45-47 for all 2B3-positive DQ beta chains.
authors.affiliation: Department of Immunohaematology, University Hospital, Leiden, The Netherlands.
authors.forename: M J
authors.initials: MJ
authors.lastname: Kenter
authors.forename: J D
authors.initials: JD
authors.lastname: Anholts
authors.forename: G M
authors.initials: GM
authors.lastname: Schreuder
authors.forename: M C
authors.initials: MC
authors.lastname: van Eggermond
authors.forename: G M
authors.initials: GM
authors.lastname: Ghyselen
authors.forename: J J
authors.initials: JJ
authors.lastname: van Rood
authors.forename: M J
authors.initials: MJ
authors.lastname: Giphart
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015342:DNA Probes; D015343:DNA Probes, HLA; D005260:Female; D005802:Genes, MHC Class II; D006683:HLA-DQ Antigens / Q000235:genetics*; D059866:HLA-DQ beta-Chains; D006650:Histocompatibility Testing; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data
chemical_list: D015342:DNA Probes; D015343:DNA Probes, HLA; D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C057615:HLA-DQbeta antigen