Consensus peptide antibodies reveal a widespread occurrence of Ca2+/lipid-binding proteins of the annexin family.
Antibodies generated against synthetic peptides that correspond to highly conserved sequence motifs in the annexins reacted with a variety of annexins from different species. These include Xenopus laevis and Drosophila melanogaster, which contain cross-reacting polypeptides of apparent Mr 34,000 and 30,000. As expected for typical annexins, the two Drosophila proteins interact in a Ca2+-dependent manner with phosphatidylserine liposomes.
Gerke
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Max Planck Institute for Biophysical Chemistry, Department of Biochemistry, Göttingen, FRG.
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D000937:Antigen-Antibody Reactions; D002135:Calcium-Binding Proteins / Q000235:genetics* / Q000276:immunology; D002460:Cell Line; D002642:Chick Embryo; D004331:Drosophila melanogaster / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D008081:Liposomes; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis*; D014981:Xenopus
D000906:Antibodies; D002135:Calcium-Binding Proteins; D008081:Liposomes; D010455:Peptides