title: Regulation of major histocompatibility class I gene expression at the level of transcription in highly oncogenic adenovirus transformed rat cells.
abstract: Highly oncogenic adenovirus type 12 (Ad12) transformed rat cells contain decreased levels of cytoplasmic and nuclear major histocompatibility (MHC) class I transcripts, a process dependent on the presence of the Ad12 E1A oncogene. Here we show that the reduction in MHC expression is due at least in part to a decreased rate of initiation of transcription of MHC class I genes. Gamma-interferon treatment of Ad12 transformed cells causes an elevation in steady-state levels of cytoplasmic MHC class I transcripts.
authors.affiliation: Department of Biochemistry, University of Leeds, UK.
authors.forename: A M
authors.initials: AM
authors.lastname: Ackrill
authors.forename: G E
authors.initials: GE
authors.lastname: Blair
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics*; D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D005786:Gene Expression Regulation; D005805:Genes, MHC Class I; D007371:Interferon-gamma / Q000494:pharmacology; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger; D051381:Rats; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D007371:Interferon-gamma