title: Sporulation of Myxococcus xanthus in liquid shake flask cultures.
abstract: When suspended in a liquid starvation medium, exponentially growing Myxococcus xanthus sporulated within 3 days. These myxospores were similar to spores developed within fruiting bodies, as determined by electron microscopy and the production of spore-specific protein S. This liquid sporulation system may be useful as a means of preparing large quantities of myxospores and extracellular fluid for biochemical studies, including isolation of chemical signals produced during the sporulation process.
authors.affiliation: Department of Microbiology, Tel Aviv University, Israel.
authors.forename: A
authors.initials: A
authors.lastname: Rosenbluh
authors.forename: E
authors.initials: E
authors.lastname: Rosenberg
mesh_terms: D001431:Bacteriological Techniques; D003470:Culture Media; D007700:Kinetics; D008854:Microscopy, Electron; D009231:Myxococcales / Q000254:growth & development / Q000502:physiology* / Q000648:ultrastructure; D013171:Spores, Bacterial / Q000502:physiology / Q000648:ultrastructure
chemical_list: D003470:Culture Media