Evaluation of trehalose-mannitol broth for differentiation of Staphylococcus epidermidis from other coagulase-negative staphylococcal species.
Trehalose-mannitol agar and various broth formulations thereof were evaluated for the differentiation of Staphylococcus epidermidis from other common coagulase-negative staphylococcal species isolated from human blood cultures. While this differentiation was accurately made on trehalose-mannitol agar in 18 h, modification of the medium to a broth formulation containing 2% each trehalose and mannitol in 0.2 ml of purple broth base provided greater than 95% accuracy when a heavy inoculum was incubated on a rotary shaker for at least 4 h.
Knapp
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CC
Department of Microbiology, Cleveland Clinic Foundation, Ohio 44195-5140.
Washington
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JA
D003470:Culture Media; D004187:Disaccharides; D006801:Humans; D008353:Mannitol; D013203:Staphylococcal Infections / Q000175:diagnosis*; D013212:Staphylococcus epidermidis / Q000302:isolation & purification*; D014199:Trehalose
D003470:Culture Media; D004187:Disaccharides; D008353:Mannitol; D014199:Trehalose