title: [Study of the role of lysine residues of the cholesterol hydroxylating cytochrome P-450 by a method of chemical modification].
abstract: Chemical modification of cytochrome P-450scc by lysine-specific reagents has been performed. Modification of the hemoprotein was shown to result in the loss of its ability to interact with adrenodoxin. With a view of identifying lysine residues involved in the interaction with adrenodoxin, cytochrome P-450scc was modified by succinic anhydride in the presence of adrenodoxin. After the removal of ferredoxin, the modification was performed with the use of a radioactively labeled reagent. Subsequent hydrolysis of the succinic hemoprotein by chymotrypsin and separation of the peptides obtained by high pressure liquid chromatography resulted in the isolation of seven chymotryptic peptides containing labeled lysine residues. These amino acid sequences were identified. The role of lysine residues of cytochrome P-450scc in complex formation with adrenodoxin is discussed.
authors.forename: T V
authors.initials: TV
authors.lastname: Adamovich
authors.forename: I A
authors.initials: IA
authors.lastname: Pikuleva
authors.forename: S A
authors.initials: SA
authors.lastname: Usanov
authors.forename: V L
authors.initials: VL
authors.lastname: Chashchin
mesh_terms: D000325:Adrenodoxin / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000378:metabolism*; D006420:Hemeproteins / Q000378:metabolism; D006868:Hydrolysis; D006900:Hydroxylation; D007202:Indicators and Reagents; D008239:Lysine / Q000378:metabolism*; D008969:Molecular Sequence Data; D013387:Succinic Anhydrides
chemical_list: D006420:Hemeproteins; D007202:Indicators and Reagents; D013387:Succinic Anhydrides; D000325:Adrenodoxin; C031801:succinic anhydride; D002786:Cholesterol Side-Chain Cleavage Enzyme; D008239:Lysine
vernacular_title: Issledovanie roli ostatkov lizina kholesteringidroksiliruiushchego tsitrokhroma P-450 metodom khimicheskoĭ modifikatsii.