Ascorbate interacts with sodium selenite to increase glutathione peroxidase activity in selenium-deficient chick duodena cultured in vitro.
Duodena from Selenium (Se)/vitamin E-depleted 19 d chick embryos were cultured in vitro for 0-30 h. The addition of sodium selenite to the culture medium was associated with increased selenium-dependent glutathione peroxidase (SeGSHpx) activity after 24 h of incubation. In the absence of Se or in the presence of sodium ascorbate supplementation alone, SeGSHpx activity showed a gradual decline over the same time period. When ascorbate was added, along with sodium selenite, SeGSHpx activity was increased earlier and to a greater extent than in the presence of Se alone. These observations show that ascorbate can influence the metabolism of sodium selenite, resulting in increased SeGSHpx activity.
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Dept. of Poultry and Avian Sciences, Cornell University, Ithaca, NY 14853.
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Corradino
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D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D002642:Chick Embryo; D004386:Duodenum / Q000187:drug effects / Q000201:enzymology*; D005979:Glutathione Peroxidase / Q000378:metabolism*; D009924:Organ Culture Techniques; D012643:Selenium / Q000172:deficiency* / Q000494:pharmacology*; D018038:Sodium Selenite; D014811:Vitamin E Deficiency / Q000378:metabolism
D005979:Glutathione Peroxidase; D012643:Selenium; D018038:Sodium Selenite; D001205:Ascorbic Acid