Neutrophil-derived oxidants mediate formyl-methionyl-leucyl-phenylalanine-induced increases in mucosal permeability in rats.
The effects of several free radical scavengers and antioxidant enzymes on neutrophil-mediated changes in mucosal permeability (measured using blood-to-lumen clearance of 51Cr-labeled ethylene-diaminetetraactate) were assessed using ileal loops perfused with N-formyl-methionyl-leucyl-phenylalanine (FMLP). Neither superoxide dismutase nor catalase reduced the FMLP-induced increase in mucosal permeability. However, manganese-loaded desferrioxamine (a superoxide dismutase mimetic), PZ51 (a glutathione peroxidase analogue), desferrioxamine (an iron chelator), or dimethylsulfoxide (a hydroxyl radical scavenger) significantly attenuated FMLP-induced mucosal damage. The results of our experiments indicate that neutrophilic oxidants are responsible for a major portion of the mucosal permeability changes induced by FMLP.
von Ritter
C
C
Department of Physiology and Biophysics, Louisiana State University Medical Center, Shreveport.
Grisham
M B
MB
Hollwarth
M
M
Inauen
W
W
Granger
D N
DN
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002860:Chromium Radioisotopes; D004492:Edetic Acid; D005609:Free Radicals; D007082:Ileum / Q000378:metabolism; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000633:toxicity*; D009504:Neutrophils / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D000975:Antioxidants; D002860:Chromium Radioisotopes; D005609:Free Radicals; D009240:N-Formylmethionine Leucyl-Phenylalanine; D004492:Edetic Acid