title: B-lymphoma cells process and present their endogenous immunoglobulin to major histocompatibility complex-restricted T cells.
abstract: Antigen-presenting B-lymphoma cells were transfected with the gene encoding the immunoglobulin lambda 2 light chain of MOPC315 cells (lambda 2(315). The lambda 2 chain is expressed on the cell surface of the transfectants together with the endogenous heavy chain. The transfectants present an idiotope of the lambda 2(315) light chain to class II-restricted T-cell clones. Recognition by the T cells requires processing of the lambda 2(315) light chain. From these data we conclude that B-lymphoma cells constitutively process and present their immunoglobulins. Secretion and reuptake of the light chain was not necessary for the presentation. Thus, B cells bear two types of idiotypes on their membrane, a native form as surface immunoglobulin and a processed form in the context of products of the major histocompatibility complex.
authors.affiliation: Basel Institute for Immunology, Switzerland.
authors.forename: S
authors.initials: S
authors.lastname: Weiss
authors.forename: B
authors.initials: B
authors.lastname: Bogen
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D005803:Genes, Immunoglobulin; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D007146:Immunoglobulin lambda-Chains / Q000235:genetics*; D008213:Lymphocyte Activation; D008223:Lymphoma / Q000276:immunology*; D008285:Major Histocompatibility Complex; D010954:Plasmacytoma / Q000235:genetics / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014162:Transfection
chemical_list: D007147:Immunoglobulin Light Chains; D007146:Immunoglobulin lambda-Chains