[Detection of toxin and evaluation of the degree of toxin-formation of Corynebacterium diphtheriae using immunoenzyme analysis].
Toxin production and the intensity of toxin formation in 265 C. diphtheriae strains circulating in different areas of the USSR have been studied by the method of the enzyme-linked immunosorbent assay (ELISA). This study has been carried out with the use of the assay system consisting of monoclonal antibodies to the COOH-area of the B-fragment of the toxin molecule adsorbed onto the surface of polystyrene plates, affinity-purified polyclonal antidiphtheria antibodies labeled with horse-radish peroxidase and substrate indicator mixture (5-aminosalicylic acid and hydrogen peroxide). Some specific features of using ELISA for the detection of C. diphtheriae toxin directly in liquid culture medium are presented. High sensitivity, specificity and good reproducibility of this method permitting the detection of C. diphtheriae toxin and the determination of the intensity of toxin formation in the C. diphtheriae strains under study are shown. The method may be recommended for practical use at health institutions.
Mazurova
I K
IK
Potemkina
E E
EE
Sviridov
V V
VV
Zaĭtsev
E M
EM
D000911:Antibodies, Monoclonal; D003353:Corynebacterium diphtheriae / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D011233:Precipitin Tests; D014118:Toxins, Biological / Q000032:analysis* / Q000096:biosynthesis
D000911:Antibodies, Monoclonal; D014118:Toxins, Biological
Detektsiia toksina i otsenka stepeni toksinoobrazovaniia Corynebacterium diphtheriae s pomoshch'iu immunofermentnogo analiza.