Bacterial antigen processing in macrophages infected with the obligate intracellular bacterium, Mycobacterium avium.
The intracellular pathway followed by Mycobacterium avium antigens was studied by immunofluorescence and immunoelectron microscopy after infection in bone marrow-derived macrophages with live or gamma ray-killed bacteria. Rabbit antiserum raised against whole sonicated bacteria was used to localise antigens. The acidity of the phagosomal compartment was also investigated by immunoelectron microscopy. Preliminary results seem to indicate the phagosomal compartment is less acidified than lysosomes and that M. avium antigens are very slowly processed.
de Chastellier
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C
Dept. Biologie Moléculaire, Institut Pasteur, Paris, France.
Lang
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T
D000818:Animals; D000942:Antigens, Bacterial / Q000302:isolation & purification*; D002478:Cells, Cultured; D007150:Immunohistochemistry; D008264:Macrophages / Q000276:immunology / Q000382:microbiology*; D009162:Mycobacterium avium / Q000276:immunology*; D010588:Phagosomes / Q000276:immunology; D013997:Time Factors
D000942:Antigens, Bacterial