title: Structure-function relationships in hemoglobin Kariya, Lys-40(C5) alpha----Glu, with high oxygen affinity. Functional role of the salt bridge between Lys-40 alpha and the beta chain COOH terminus.
abstract: The epsilon-amino group of Lys-40 alpha forms a salt bridge with the alpha-carboxyl group of beta chain in deoxyhemoglobin and is considered to impose a constraint upon hemoglobin tetramer, stabilizing the T quaternary structure. Hb Kariya, in which Lys-40 alpha is replaced by Glu, provides a unique opportunity to investigate the functional role of this salt bridge. Hb Kariya showed oxygen binding properties characterized by a high affinity, diminished cooperativity, a reduced alkaline Bohr effect, and a decreased effect of phosphates upon oxygen affinity. In deoxyHb Kariya the reactivity of the sulfhydryl groups of cysteins-93 beta with 4,4'-dipyridine disulfide was profoundly enhanced, being comparable to that for normal oxyhemoglobin (oxyHb A). The Soret band spectra, UV derivative spectra, and UV oxyminus-deoxy difference spectra indicated that oxyHb Kariya assumes a quaternary structure similar to that of oxyHb A whereas the T structure of deoxyHb Kariya is destabilized, and Hb Kariya remains predominantly in the R state upon deoxygenation. Resonance Raman scattering by deoxyHb Kariya showed that the Fe-N epsilon(proximal His) bond is less stretched than that of deoxyHb A. These experimental results provide structural basis for explaining the oxygen binding characteristics of Hb Kariya and further give direct evidence that the intersubunit salt bridge between Lys-40 alpha and the beta chain COOH terminus actually contributes to stabilization of the T quaternary structure, thereby playing a key role in cooperative oxygen binding by hemoglobin. The nature of another salt bridge between Asp-94 beta and the COOH-terminal His of beta chain was also discussed in comparison with the salt bridge involving Lys-40 alpha.
authors.affiliation: Department of Physiochemical Physiology, Medical School, Osaka University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Imai
authors.forename: A
authors.initials: A
authors.lastname: Tsuneshige
authors.forename: T
authors.initials: T
authors.lastname: Harano
authors.forename: K
authors.initials: K
authors.lastname: Harano
mesh_terms: D019794:2,3-Diphosphoglycerate; D003545:Cysteine / Q000378:metabolism; D004163:Diphosphoglyceric Acids / Q000494:pharmacology; D004220:Disulfides; D006441:Hemoglobin A / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism; D006455:Hemoglobins, Abnormal / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008239:Lysine; D046911:Macromolecular Substances; D010100:Oxygen / Q000378:metabolism*; D010833:Phytic Acid / Q000494:pharmacology; D011725:Pyridines; D013056:Spectrophotometry, Ultraviolet; D013059:Spectrum Analysis, Raman; D013329:Structure-Activity Relationship; D013439:Sulfhydryl Reagents
chemical_list: D004163:Diphosphoglyceric Acids; D004220:Disulfides; D006454:Hemoglobins; D006455:Hemoglobins, Abnormal; D046911:Macromolecular Substances; D011725:Pyridines; D013439:Sulfhydryl Reagents; D019794:2,3-Diphosphoglycerate; C007542:4,4'-dipyridyl disulfide; D010833:Phytic Acid; C038013:hemoglobin Kariya; C100003:deoxyhemoglobin; D006441:Hemoglobin A; D008239:Lysine; D003545:Cysteine; D010100:Oxygen