title: [Usefulness of 5 microbiological markers for the differentiation of strains of Klebsiella ozaenae causing an outbreak of nosocomial infection].
abstract: Five different microbiological markers (biotyping, antimicrobial susceptibility, heavy-metal susceptibility, klebocin susceptibility and number of extrachromosomal DNA fragments) were utilized in order to distinguish between Klebsiella ozaenae strains arising from a nosocomial infection outbreak and strains of some species isolated from hospital environment. Antimicrobial susceptibility pattern to 13 different antibiotics was the most reliable maker, because 85% of the outbreak strains shown resistance to 8-10 antibiotics and only two strains of hospital environment had similar pattern. Sensitivity for this marker was 85.0% specificity 93.5% and positive predictive value of 89.5%. Other markers were less efficient with a positive predictive value of 79.2%, for biotyping, 70.4% for klebocin susceptibility, 69.2% for number of extrachromosomal DNA fragments and 60.6% for heavy-metals susceptibility. We discuss the applicability of each one of these markers in different circumstances.
authors.affiliation: Laboratorio de Enfermedades Infecciosas y Parasitarias, Subjefatura de Investigación (IMSS).
authors.forename: M
authors.initials: M
authors.lastname: García-Pérez
authors.forename: J
authors.initials: J
authors.lastname: Castellanos
authors.forename: H
authors.initials: H
authors.lastname: Guiscafre
authors.forename: S
authors.initials: S
authors.lastname: Gionno
authors.forename: O
authors.initials: O
authors.lastname: Muñoz
mesh_terms: D001430:Bacteriocins / Q000494:pharmacology; D015415:Biomarkers; D003428:Cross Infection / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis; D004196:Disease Outbreaks; D006801:Humans; D007709:Klebsiella / Q000145:classification* / Q000302:isolation & purification; D007710:Klebsiella Infections / Q000382:microbiology*; D008670:Metals / Q000494:pharmacology; D008800:Mexico; D008826:Microbial Sensitivity Tests
chemical_list: D001430:Bacteriocins; D015415:Biomarkers; D004269:DNA, Bacterial; D008670:Metals; C019447:klebocin
vernacular_title: Utilidad de cinco marcadores microbiológicos para la diferenciación de cepas de Klebsiella ozaenae causantes de un brote de infección nosocomial.