Detection of antibodies to extractable nuclear antigens using calf thymus and rabbit thymus. A comparative study of 1000 consecutive anti-nuclear antibody positive patients.
A comparison between calf thymus extract and rabbit thymus extract to determine their suitability for clinical laboratories in detecting antibodies to extractable nuclear antigens (ENA) was undertaken. Calf thymus extract (CTE) detected 90% more ENA positive patients and 155% more lines than rabbit thymus extract (RTE). In contrast to RTE, CTE resolved Sm and RNP into two distinct lines, had superior stability at -80 degrees C and produced greater clarity of definition of precipitin lines.
Collins
R J
RJ
Department of Pathology, Royal Brisbane Hospital, Herston, Australia.
Neil
J C
JC
Druery
L N
LN
Wilson
R J
RJ
D000818:Animals; D000974:Antibodies, Antinuclear / Q000032:analysis*; D001324:Autoantigens / Q000276:immunology*; D002417:Cattle; D004306:Dose-Response Relationship, Immunologic; D005779:Immunodiffusion; D007123:Immunoelectrophoresis, Two-Dimensional; D066298:In Vitro Techniques; D011817:Rabbits; D012261:Ribonucleoproteins / Q000276:immunology; D013950:Thymus Gland; D013997:Time Factors
D000974:Antibodies, Antinuclear; D001324:Autoantigens; D012261:Ribonucleoproteins