title: Duplication of the tuf gene: a new insight into the phylogeny of eubacteria.
abstract: The conservation and duplication of the tuf gene encoding the elongation factor EF-Tu were used to define phylogenetic relationships among eubacteria. When the tufA gene of Escherichia coli was used as a probe in hybridization experiments, duplicate tuf genes were found in gram-negative bacteria from three major phyla: purple bacteria, bacteroides, and cyanobacteria. Only a single copy of tuf was found in gram-positive bacteria, including mycobacteria and mycoplasmas. Gram-positive clostridia were found to carry two copies of tuf.
authors.affiliation: Department of Clinical Microbiology, Hebrew University--Hadassah Medical School, Jerusalem, Israel.
authors.forename: S
authors.initials: S
authors.lastname: Sela
authors.forename: D
authors.initials: D
authors.lastname: Yogev
authors.forename: S
authors.initials: S
authors.lastname: Razin
authors.forename: H
authors.initials: H
authors.lastname: Bercovier
mesh_terms: D001439:Bacteroides / Q000235:genetics*; D015139:Blotting, Southern; D000458:Cyanobacteria / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005051:Eubacterium / Q000235:genetics*; D005798:Genes, Bacterial; D009693:Nucleic Acid Hybridization; D010444:Peptide Elongation Factor Tu / Q000235:genetics*; D010802:Phylogeny
chemical_list: D010444:Peptide Elongation Factor Tu