title: A new method for determining the heat capacity change for protein folding.
abstract: In order to use results from calorimetry or thermal unfolding curves to estimate the free energy change for protein unfolding at 25 degrees C, it is necessary to know the change in heat capacity for unfolding, delta Cp. We describe a new method for measuring delta Cp which is based on results from urea and thermal unfolding curves but does not require a calorimeter. We find that delta Cp = 1650 +/- 200 cal/(deg.mol) for the unfolding of ribonuclease T1 and that delta Cp = 2200 +/- 300 cal/(deg.mol) for the unfolding of ribonuclease A.
authors.affiliation: Biochemistry Department, Texas A&M University, College Station 77843.
authors.forename: C N
authors.initials: CN
authors.lastname: Pace
authors.forename: D V
authors.initials: DV
authors.lastname: Laurents
mesh_terms: D002151:Calorimetry; D004722:Endoribonucleases; D011487:Protein Conformation; D011489:Protein Denaturation; D006163:Ribonuclease T1; D012259:Ribonuclease, Pancreatic; D013816:Thermodynamics; D014508:Urea
chemical_list: D014508:Urea; D004722:Endoribonucleases; D006163:Ribonuclease T1; D012259:Ribonuclease, Pancreatic