Short-term exposure to nitrogen dioxide enhances susceptibility to murine respiratory mycoplasmosis and decreases intrapulmonary killing of Mycoplasma pulmonis.
In C57BL/6N and C3H/HeN mice known to be free of all murine pathogens and matched for age, sex, microbiologic, and environmental factors, exposure to NO2 for 4 h prior to exposure to infectious aerosols of Mycoplasma pulmonis resulted in potentiation of murine respiratory mycoplasmosis (MRM). In the C57BL/6N mice, NO2 increased the incidence of death, incidence of gross lung lesions, and incidence of microscopic lung lesions, but did not increase the incidence of infection in the lungs. Nitrogen dioxide affected the C3H/HeN mice (a strain known to be more susceptible than the C57BL/6N strain to MRM) similarly, with the exception that the incidence of death and microscopic lesions were not affected in this strain at the concentrations of M. pulmonis used. Exposure to the oxidant also increased the severity of microscopic lesions and the numbers of Mycoplasma organisms in the lungs of both mouse strains. Thus, NO2 appeared to affect host lung defense mechanisms responsible for limiting the extent of infection. The NO2 exposure level required to produce potentiation varied with the genetic background of the host, the number of Mycoplasma organisms administered, and the end point measured. In further experiments in C57BL/6N mice, exposure to 5 or 10 ppm of NO2 for 4 h prior to infection with aerosolized, radiolabeled M. pulmonis reduced clearance of these organisms from the lungs over a 72-h time period. Nitrogen dioxide exposure did not change the rate of physical removal of Mycoplasma organisms from the lung. Reduced clearance was due to impaired intrapulmonary killing of Mycoplasma organisms in NO2-exposed mice.(ABSTRACT TRUNCATED AT 250 WORDS)
Parker
R F
RF
Department of Comparative Medicine and Microbiology, School of Medicine, University of Alabama, Birmingham 35294.
Davis
J K
JK
Cassell
G H
GH
White
H
H
Dziedzic
D
D
Blalock
D K
DK
Thorp
R B
RB
Simecka
J W
JW
D000280:Administration, Inhalation; D000336:Aerosols; D000818:Animals; D004198:Disease Susceptibility / Q000139:chemically induced; D005260:Female; D008168:Lung / Q000276:immunology; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D009177:Mycoplasma pneumoniae / Q000276:immunology; D009585:Nitrogen Dioxide / Q000008:administration & dosage / Q000633:toxicity*; D011019:Pneumonia, Mycoplasma / Q000209:etiology* / Q000401:mortality
D000336:Aerosols; D009585:Nitrogen Dioxide