Localization of the replication origin of plasmid pE194.
The pE194 replication origin was localized to a 265-base-pair interval by analyzing the ability of purified pE194 restriction fragments to direct replication of heterologous plasmids. Replication was dependent upon RepF protein supplied in trans. The origin region contained a GC-rich dyad symmetry which may serve as the RepF target.
Dempsey
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LA
Department of Microbiology, Public Health Research Institute, New York, New York 10016.
Dubnau
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DA
D001412:Bacillus subtilis / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D011815:R Factors; D012045:Regulatory Sequences, Nucleic Acid; D012093:Replicon; D015183:Restriction Mapping; D013210:Staphylococcus / Q000235:genetics; D014170:Transformation, Genetic
D004269:DNA, Bacterial