title: Quantification of the putative neurotoxin 2-amino-3-(methylamino)propanoic acid (BMAA) in cycadales: analysis of the seeds of some members of the family Cycadaceae.
abstract: Over the past 30 years there have been attempts to link the unusually high incidence of amyotrophic lateral sclerosis (ALS) among the Chamorros native to the island of Guam to the consumption of the seeds of Cycas circinalis L., the false sago palm. In support of this relationship it was recently shown that, when given to primates, 2-amino-3-(methylamino)-propanoic acid (BMAA), a minor cycad component, can cause selective degeneration of upper and lower motor neurons in the spinal cord and clinical features similar to those of ALS. In order to test the relationship between ALS and cycads, we have developed a sensitive and precise gas chromatographic/mass spectrometric (GC/MS) assay for BMAA which allows direct assessment of the BMAA content in foods and is directly applicable to the assay of BMAA in biologic tissues and fluids. After the addition of a deuterated isotopomer as an internal standard and transesterication with 2-methyl-1-propanol, BMAA was extracted into dichloromethane and then acylated with pentafluoropropionic anhydride before GC/MS. This method permits precise quantification of BMAA in the low picogram/sample range. Direct quantification of the BMAA content in the female gametophyte tissue (endosperm) of a range of cycad seeds collected from Guam confirmed the presence of BMAA at levels of approximately 1 g/g (dry weight). The presence of BMAA in the seed extract was confirmed after derivatization of an aliquot of the extract and GC/MS analysis in the scanning mode. BMAA was found to be present, albeit at lower levels, in the endosperm of the seeds of C. revoluta (0.32 mg/g) and C. media (0.29 mg/g).
authors.forename: M W
authors.initials: MW
authors.lastname: Duncan
authors.forename: I J
authors.initials: IJ
authors.lastname: Kopin
authors.forename: J S
authors.initials: JS
authors.lastname: Crowley
authors.forename: S M
authors.initials: SM
authors.lastname: Jones
authors.forename: S P
authors.initials: SP
authors.lastname: Markey
mesh_terms: D000599:Amino Acids, Diamino / Q000032:analysis*; D000690:Amyotrophic Lateral Sclerosis / Q000139:chemically induced*; D008401:Gas Chromatography-Mass Spectrometry; D006141:Guam; D009498:Neurotoxins / Q000032:analysis*; D010945:Plants, Edible; D012639:Seeds / Q000032:analysis*
chemical_list: D000599:Amino Acids, Diamino; D009498:Neurotoxins