title: Transformation of Penicillium chrysogenum with selection for increased resistance to benomyl.
abstract: Incubation of protoplasts of Penicillium chrysogenum with the plasmid pBT-3 permitted selection of transformants on the basis of increased resistance to the anti-fungal agent benomyl. Transformants were obtained at a frequency of 1-2 per microgram of DNA. Southern analysis revealed that transformation had occurred by integration of vector sequences into the host genome.
authors.affiliation: Centre for Biotechnology, Imperial College of Science Technology and Medicine, London, U.K.
authors.forename: T M
authors.initials: TM
authors.lastname: Picknett
authors.forename: G
authors.initials: G
authors.lastname: Saunders
mesh_terms: D001542:Benomyl / Q000494:pharmacology*; D015139:Blotting, Southern; D002219:Carbamates / Q000494:pharmacology*; D004352:Drug Resistance, Microbial / Q000235:genetics; D005800:Genes, Fungal; D008938:Mitosis; D010407:Penicillium / Q000235:genetics*; D010408:Penicillium chrysogenum / Q000187:drug effects / Q000235:genetics*; D010957:Plasmids; D011523:Protoplasts; D014170:Transformation, Genetic; D014404:Tubulin / Q000235:genetics
chemical_list: D002219:Carbamates; D014404:Tubulin; D001542:Benomyl