title: Structure of cDNA of cytosolic aspartate aminotransferase of chicken and its expression in E. coli.
abstract: The structure of the mRNA of chicken cytosolic aspartate aminotransferase has been determined by analysis of cDNA and genomic clones. Two transcripts of different length were found that appear to arise from the alternate use of 2 polyadenylation signals in the 3' untranslated region. The expression product of the full-length construct in E. coli proved to be catalytically active and possessed the expected molecular weight.
authors.affiliation: Biochemisches Institut der Universität Zürich, Switzerland.
authors.forename: U
authors.initials: U
authors.lastname: Mattes
authors.forename: R
authors.initials: R
authors.lastname: Jaussi
authors.forename: M
authors.initials: M
authors.lastname: Ziak
authors.forename: N
authors.initials: N
authors.lastname: Juretic
authors.forename: J M
authors.initials: JM
authors.lastname: Lindenmann
authors.forename: P
authors.initials: P
authors.lastname: Christen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001219:Aspartate Aminotransferases / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D015152:Blotting, Northern; D002645:Chickens; D003001:Cloning, Molecular; D003600:Cytosol / Q000201:enzymology*; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D004926:Escherichia coli / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping
chemical_list: D015342:DNA Probes; D012333:RNA, Messenger; D004247:DNA; D001219:Aspartate Aminotransferases