Isolation of an IgH gene circular DNA clone from human bone marrow.
Circular DNA was obtained from human bone marrow. Then a phage library was prepared and screened by use of two probes of the IgH gene; 5'-DHQ52, containing the 5' flanking region of DHQ52, and JH4.3, containing the sequence from JH3 to the 3' flanking region of JH6. One clone, HBMC-1, that was DHQ52+JH4.3- was obtained. HBMC-1 had the germline IgH region upstream of JH1 and the 3' flanking region of DXP1. A recombination signal sequence flanking the 5' side of the JH1 segment was attached to the recombination signal sequence flanking the 3' side of DXP1 forming a head-to-head structure of two 7mers with 10 nucleotides in-between. HBMC-1 is thus considered to be a circular DNA deleted as a consequence of DXP1-JH1 joining of the IgH gene.
Abe
M
M
Department of Oncology, Nagasaki University School of Medicine, Japan.
Shiku
H
H
D001483:Base Sequence; D015139:Blotting, Southern; D001853:Bone Marrow / Q000276:immunology*; D003001:Cloning, Molecular; D004270:DNA, Circular / Q000235:genetics*; D005803:Genes, Immunoglobulin; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D004270:DNA, Circular; D007143:Immunoglobulin Heavy Chains