Murine V kappa gene expression does not follow the VH paradigm.
V kappa gene family expression among LPS-reactive murine B lymphocytes, unlike that of VH gene families, is not proportional to genomic complexity, i.e., nonstoichiometric. Furthermore, no positional bias for the overexpression of J-proximal V kappa genes (V kappa 21) is observed among neonatal B lymphocytes. Yet, the V kappa 1 and V kappa 9 families located in the center of V kappa locus are preferentially used by neonatal B splenocytes. Thus, the mechanisms of V kappa gene rearrangement and expression appear to differ significantly from those controlling the VH locus.
Kaushik
A
A
Department of Microbiology, Mount Sinai School of Medicine, New York 10029.
Schulze
D H
DH
Bona
C
C
Kelsoe
G
G
D000818:Animals; D000831:Animals, Newborn; D015342:DNA Probes; D005786:Gene Expression Regulation; D005803:Genes, Immunoglobulin; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D009693:Nucleic Acid Hybridization
D015342:DNA Probes; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D007145:Immunoglobulin kappa-Chains