Inhibition of tumour cell adhesion by anti-metastatic polypeptide containing a repetitive Arg-Gly-Asp sequence.
Anti-cell adhesive activity was examined by the synthetic polypeptide, containing repetitive Arg-Gly-Asp sequence of cell attachment site from fibronectin, poly (Arg-Gly-Asp). The attachment of tumour cells to fibronectin substrate was specifically inhibited by adding poly (Arg-Gly-Asp) in cell surface receptor-mediated and divalent cation-dependent manners, but not by unrelated peptides. In our previous study, the lung metastatic formation of tumour cells was dramatically reduced by intravenous co-injection of anti-cell adhesive poly (Arg-Gly-Asp) with B16-BL6 melanoma cells. These findings suggest that polypeptide-mediated inhibition of pulmonary metastasis is partly due to interference with tumour cell adhesion to the substrates including fibronectin in target organs or tissues.
Murata
J
J
Institute of Immunological Science, Hokkaido University, Sapporo, Japan.
Saiki
I
I
Iida
J
J
Azuma
I
I
Kawahara
H
H
Nishi
N
N
Tokura
S
S
D000595:Amino Acid Sequence; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects*; D005260:Female; D005353:Fibronectins / Q000378:metabolism; D008546:Melanoma, Experimental / Q000378:metabolism* / Q000556:secondary; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis; D009842:Oligopeptides / Q000378:metabolism / Q000494:pharmacology*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D012091:Repetitive Sequences, Nucleic Acid; D013379:Substrate Specificity; D014407:Tumor Cells, Cultured
D005353:Fibronectins; D009842:Oligopeptides; D010455:Peptides; C047981:arginyl-glycyl-aspartic acid