Direct effects of estrogen on c-fos and c-myc protooncogene expression and cellular proliferation in human breast cancer cells.
Estrogen induces DNA synthesis to a lesser degree, but with similar kinetics compared to insulin-like growth factors when added to quiescent MCF7 human breast cancer cells. Moreover, this steroid rapidly induces expression of the growth-related c-fos and c-myc protooncogenes. The induction was shown to be a direct effect, independent of protein synthesis. These results demonstrate that no growth factor production is involved in estrogen-induced protooncogene expression, and that these direct effects on gene expression may be an important step in estrogen-induced mitogenesis.
van der Burg
B
B
Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Utrecht.
van Selm-Miltenburg
A J
AJ
de Laat
S W
SW
van Zoelen
E J
EJ
D001943:Breast Neoplasms / Q000378:metabolism*; D002455:Cell Division; D004261:DNA Replication; D004958:Estradiol / Q000502:physiology*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007328:Insulin / Q000502:physiology; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016760:Proto-Oncogene Proteins c-fos; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger / Q000096:biosynthesis; D013997:Time Factors; D014407:Tumor Cells, Cultured
D007328:Insulin; D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D004958:Estradiol