Lytic effect of Pseudomonas aeruginosa elastase on gram-positive and -negative bacteria.
Elastase of Pseudomonas aeruginosa P3 caused lysis of freshly grown cells of Gram-positive (Staphylococcus aureus and S. epidermidis) and Gram-negative (Proteus vulgaris and Salmonella typhi) bacteria. Heat killed and lyophilized Gram-negative bacteria showed higher sensitivity to elastase. Both Gram-negative and Gram-positive bacteria were lysed maximally by elastase at pH 8.0. At this pH, the activity of elastase was maximal in Tris-HCl and glycine-NaOH buffers followed by Tris-maleate and Cacodylate buffers.
Kaur
M
M
Department of Microbiology, Panjab University, India.
Gupta
M
M
Tripathi
K K
KK
Gupta
K G
KG
D001433:Bacteriolysis; D005612:Freeze Drying; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D010196:Pancreatic Elastase / Q000302:isolation & purification / Q000494:pharmacology / Q000502:physiology*; D011550:Pseudomonas aeruginosa / Q000201:enzymology*
D010196:Pancreatic Elastase