Extracellular phospholipase A2 activity in cell free peritoneal lavage fluid from mice with zymosan peritonitis.
The kinetics and appearance of extracellular phospholipase A2 (PLA2) activity and its relationship to the appearance of arachidonic acid (AA) metabolites in the peritoneal cavity of mice injected with zymosan is described. AA metabolites levels including leukotriene C4 (LTC4) were maximum 15-30 min after zymosan. Peak PLA2 activity was also found 15-30 min after zymosan and was significantly increased above levels found in saline control exudates (3.83 +/- 0.89 and 0.35 +/- 0.11, respectively, p less than or equal to 0.05). Results show that an extracellular PLA2 is present in zymosan peritonitis.
Gans
K R
KR
Medical Products Department, E. I. Du Pont de Nemours & Co. Inc., Wilmington, DE 19880-400.
Lundy
S R
SR
Dowling
R L
RL
Stevens
T M
TM
Kerr
J S
JS
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D008297:Male; D051379:Mice; D010538:Peritonitis / Q000139:chemically induced / Q000276:immunology* / Q000378:metabolism; D010740:Phospholipases / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D013189:SRS-A / Q000378:metabolism; D007507:Therapeutic Irrigation; D013997:Time Factors; D015054:Zymosan
D001095:Arachidonic Acids; D013189:SRS-A; D016718:Arachidonic Acid; D015054:Zymosan; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2