Evaluation of the human sperm acrosome reaction using a monoclonal antibody, GB24, and fluorescence-activated cell sorter.
GB24 is a mouse monoclonal antibody raised against human trophoblast microvilli, which recognizes an antigenic determinant on the acrosomal region of the human sperm head. By indirect immunofluorescence, reactivity of GB24 could not be detected on freshly ejaculated spermatozoa but was strongly positive after sperm permeabilization with acetone. On viable, motile spermatozoa, reactivity appeared after induction of the acrosomal reaction with the calcium ionophore A23187. These results suggest that the antigen recognized by GB24 is present on the inner acrosomal membrane. A quantitative evaluation assay of the acrosome reaction on viable spermatozoa by flow cytometry using GB24 and indirect immunofluorescence is proposed.
Fénichel
P
P
INSERM U210, Faculté de Médecine, France.
Hsi
B L
BL
Farahifar
D
D
Donzeau
M
M
Barrier-Delpech
D
D
Yehy
C J
CJ
D000177:Acrosome / Q000276:immunology / Q000502:physiology*; D000911:Antibodies, Monoclonal; D000001:Calcimycin / Q000494:pharmacology; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast; D013075:Sperm Capacitation; D013094:Spermatozoa / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D000001:Calcimycin