Potentiation of N-methyl-N'-nitro-N-nitrosoguanidine-induced O6-methylguanine-DNA-methyltransferase activity in a rat hepatoma cell line by poly (ADP-ribose) synthesis inhibitors.
The O6-methylguanine-DNA-methyltransferase (transferase) activity in a rat hepatoma cell line (H4 cells) is enhanced as a response to DNA damaging agents. To study whether poly (ADP-ribosylation) is involved in this induction, the cells were treated with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) that induces the transferase activity and stimulates poly (ADP-ribose) synthesis. Addition of poly (ADP-ribose) polymerase inhibitors enhanced the transferase increase induced by MNNG. The influence of the inhibitors on the transferase induction was dose and time-dependent. The results suggest that poly (ADP-ribose) is involved in the induction of this protein.
Lefebvre
P
P
Groupe Radiochimie de l'ADN (U 247 INSERM), Institut Gustave Roussy, Villejuif, France.
Laval
F
F
D000818:Animals; D001549:Benzamides / Q000494:pharmacology*; D004249:DNA Damage; D004260:DNA Repair; D008114:Liver Neoplasms, Experimental / Q000201:enzymology*; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology*; D008780:Methyltransferases / Q000378:metabolism*; D019853:O(6)-Methylguanine-DNA Methyltransferase; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D051381:Rats; D014407:Tumor Cells, Cultured
D001549:Benzamides; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D008769:Methylnitronitrosoguanidine; C025160:3-aminobenzamide; D008780:Methyltransferases; D019853:O(6)-Methylguanine-DNA Methyltransferase