Determination of dopamine, norepinephrine, and related trace amines by prechromatographic derivatization with naphthalene-2,3-dicarboxaldehyde.
Dopamine, norepinephrine, octopamine, tyramine, and 3,4-dihydroxybenzylamine react readily with naphthalene-2,3-dicarboxaldehyde in the presence of cyanide ion under mild conditions to give highly fluorescent cyanobenz[f]isoindole (CBI) products. The CBI products exhibit good solution chemical stability. The high-fluorescence quantum efficiency of the CBI fluorophore and the ability to excite these adducts in the visible region (420-450 nm) enhance the sensitivity and selectivity of this derivatization detection technique. The CBI products of catecholamines and "trace" amines are readily separated by reverse-phase HPLC giving detection limits in the 20 to 60 fmol range (S/N = 3). A prechromatographic derivatization HPLC assay for the trace analysis of dopamine and norepinephrine in urine is described.
Kawasaki
T
T
Center for Bioanalytical Research, University of Kansas, Lawrence 66045.
Higuchi
T
T
Imai
K
K
Wong
O S
OS
D001679:Biogenic Amines / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004298:Dopamine / Q000032:analysis* / Q000652:urine; D005453:Fluorescence; D007700:Kinetics; D009281:Naphthalenes; D009638:Norepinephrine / Q000032:analysis* / Q000652:urine; D009655:Octopamine / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D014439:Tyramine / Q000032:analysis
D001679:Biogenic Amines; D009281:Naphthalenes; D009655:Octopamine; C027717:2,3-naphthalenedicarboxaldehyde; D004298:Dopamine; D009638:Norepinephrine; D014439:Tyramine