Steroid conjugate formed by human endometrium.
Progesterone -6,7-3H (P*) was incubated in minces of human secretory and proliferative endometrium in absence as well as in presence of 10 and 100 micrograms/ml of unlabelled progesterone (P), in Eagle's Culture Medium throughout 72h. The following metabolites of P* were found in culture media: 1. C-21 derivatives of P* reduced at C-5, and C-20 positions. Also, a 3 beta-hydroxy-5 alpha pregnane-20-One conjugated to a glucuronic acid moiety was identified. 2. Concentrations of water-soluble derivative accounted for 21% and 29% of the recovered radioactivity in proliferative and secretory endometria, respectively. 3. After beta-glucuronidase cleavage, 80% to 95% of the water soluble derivatives were released as free steroids. 4. Approximately 60% corresponded to 3 beta -hydroxy- 5 alpha pregnane-20-one of the pooled water extracts. 5. Alson, 17% and 13% as well as 9% and 8% recoveries under 10 and 100 micrograms/P were observed in proliferative and secretory endometria, respectively. Glucuronidation seems to be a compensatory route to metabolize P and P* in human endometrium as might also occur in other species.
Garzón
P
P
Navarro-Ruíz
A
A
García-Estrada
J
J
Gallegos
A
A
D001711:Biotransformation; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D005965:Glucuronates / Q000378:metabolism*; D020723:Glucuronic Acid; D006001:Glycoconjugates / Q000096:biosynthesis*; D006801:Humans; D011280:Pregnanolone / Q000378:metabolism; D011374:Progesterone / Q000378:metabolism*
D005965:Glucuronates; D006001:Glycoconjugates; D011374:Progesterone; D020723:Glucuronic Acid; D011280:Pregnanolone