[DNA synthesis in cells of human cadavers].
Punch biopsies of human cadavers were incubated in vitro with radioactively labeled thymidine (3H-TdR, 14C-TdR) to get information on postmortal cytokinetics. The investigation established that 3H-TdR really was incorporated in DNA by cells of human cadavers. Basal cells of epidermis as well bone marrow cells of cadavers stored at 20 degrees C incorporated 3H-TdR during a storage time of at least 32 hours. Storing the cadavers at 4 degrees C DNA synthesis in epidermic cells was demonstrated for more than 77 hours. While spermatogonia incorporated 3H-TdR during the early postmortal period of 8 hours neither in bowel nor in splenic cells a postmortal DNA synthesis was seen. Systemic investigations were done using punch biopsies taken from the extensor side of the thigh of 45 human cadavers stored at 4 degrees C. The labeling index (mean: 2.53 +/- 0.79%) over a postmortal period of more than 70 hours and the synthesis time determined by the double labeling procedure over a period of at least 30 hours remained essentially unchanged and were comparable with live humans. The generation time decreased with increasing storage time--but not statistically relevant differences, however, were established at the 1% level. As well no statistically relevant differences were established by correlating the kinetic data according to sex, age and site.
Oehmichen
M
M
Institut für Rechtsmedizin der Universität Köln.
Frasunek
J
J
Zilles
K
K
D001345:Autoradiography; D001706:Biopsy; D001853:Bone Marrow / Q000473:pathology; D004261:DNA Replication; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000473:pathology; D008297:Male; D011180:Postmortem Changes; D012867:Skin / Q000473:pathology; D013154:Spleen / Q000473:pathology; D013737:Testis / Q000473:pathology
DNS-Synthese von Zellen menschlicher Leichen.