[Expression of synthetic human angiogenin gene in E. coli in cleaved beta-galactosidase-angiogenin protein].
A synthetic gene coding for human angiogenin was cloned in pUR290 plasmid in frame with beta-galactosidase, both parts of the resultant fused protein being joined through an Asp-Pro sequence. The fused protein, synthesised in E. coli cells upon IPTG induction and isolated as inclusion bodies, possessed angiogenic activity on the chick chorioallantois membrane and was cleaved upon acid treatment to yield free angiogenin.
Kovalenko
S P
SP
Lisnyak
I A
IA
Mertvetsov
N P
NP
D000595:Amino Acid Sequence; D043925:Angiogenesis Inducing Agents; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005696:Galactosidases / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D005813:Genes, Synthetic; D006801:Humans; D006868:Hydrolysis; D008969:Molecular Sequence Data; D010957:Plasmids; D011506:Proteins / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012259:Ribonuclease, Pancreatic; D001616:beta-Galactosidase / Q000235:genetics*
D043925:Angiogenesis Inducing Agents; D011506:Proteins; D011993:Recombinant Fusion Proteins; C046366:angiogenin; D012259:Ribonuclease, Pancreatic; D005696:Galactosidases; D001616:beta-Galactosidase
Ekspressiia sinteticheskogo gena angiogenina cheloveka i E. coli v sostave slitogo belka beta-galaktozidaza-angiogenin.