Effects of dexamethasone on glucose transport by skeletal muscles of obese (ob/ob) mice.
Adrenalectomy normalized basal and insulin-stimulated glucose transport in the perfused hindquarter of the obese (ob/ob) mouse. Dexamethasone treatment of adrenalectomized obese mice for 9 days significantly reduced both the basal and insulin-stimulated uptake of (3H)-2-deoxy-D-glucose in hindlimbs of adrenalectomized mice to levels similar to those of intact ob/ob mice. Five hours after injection of dexamethasone in vivo and following 5 hours of perfusion in vitro there was a 10 percent reduction in basal 2-deoxy-D-glucose uptake in both groups. In muscles perfused with dexamethasone in vitro, the stimulation of 2-deoxy-D-glucose uptake by insulin was greater than in those muscles perfused with vehicle alone. Five hours of treatment in vivo also resulted in a significant increase in liver tyrosine aminotransferase activity, and a decrease in muscle glycogen synthetase activity ratio. These data suggest that the effect of glucocorticoids on lowering glucose transport in the skeletal muscle of obese ob/ob mice is a direct one.
Ohshima
K
K
Section of Diabetes and Clinical Nutrition, School of Medicine, University of Southern California, Los Angeles 90033.
Shargill
N S
NS
Chan
T M
TM
Bray
G A
GA
D000315:Adrenalectomy; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D003907:Dexamethasone / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism*; D006614:Hindlimb; D008297:Male; D051379:Mice; D008820:Mice, Obese; D009132:Muscles / Q000187:drug effects* / Q000378:metabolism; D009765:Obesity / Q000378:metabolism*
D003907:Dexamethasone; D005947:Glucose