title: DNA grading of oral squamous carcinomas. A preliminary report.
abstract: The prognostic validity of the DNA-malignancy-grade (DNA-MG), ranging on a continuous scale from 0.00 to 3.00 was tested in a preliminary study on 7 patients with oral squamous cell carcinomas. Monolayer smears were prepared after a cell separation procedure from paraffin-embedded surgical tumor specimens. Feulgen staining was performed automatically in a modified Shandon staining machine. A TV image analysis system with an automatic microscope (TAS plus, Leitz, FRG) was used for DNA measurements. The DNA-MG revealed a strong correlation with the patients prognosis. 3 patients who died after having a tumor relapse (mean survival = 10 months) had DNA grades greater than 1.40, whereas patients who survived without having a tumor relapse (mean survival = 53 months) revealed DNA grades below 1.00. Additionally, the DNA-malignancy-grade was closely correlated with the histopathologic malignancy grades.
authors.affiliation: Department of Pathology, Aachen University of Technology, FRG.
authors.forename: R
authors.initials: R
authors.lastname: Chatelain
authors.forename: B
authors.initials: B
authors.lastname: Hoffmeister
authors.forename: F
authors.initials: F
authors.lastname: Härle
authors.forename: A
authors.initials: A
authors.lastname: Böcking
authors.forename: C
authors.initials: C
authors.lastname: Mittermayer
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000648:ultrastructure*; D004273:DNA, Neoplasm / Q000648:ultrastructure*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000648:ultrastructure*; D009364:Neoplasm Recurrence, Local; D009367:Neoplasm Staging; D011379:Prognosis; D013690:Television
chemical_list: D004273:DNA, Neoplasm