Human intestinal lactase-phlorizin hydrolase: isolation and preparation of a specific antiserum.
Human intestinal lactase-phlorizin hydrolase (lactase) was selectively isolated with monospecific polyclonal antibodies to rat lactase. In addition to their immunologic similarities indicated by this isolation, human and rat lactase have similar kinetic characteristics but different subunit structure when analyzed by gel electrophoresis under reducing conditions. Rabbits immunized by injecting human lactase complexed with anti-rat lactase produced specific antibodies to human lactase that exhibited little cross-reactivity to the rat enzyme. The simple single-step procedure allows isolation of human lactase in high purity from small biologic samples and preparation of specific antisera to the human enzyme.
Castillo
R O
RO
Department of Pediatrics, Stanford University Medical Center, CA 94305.
Kwong
L K
LK
Reisenauer
A M
AM
Tsuboi
K K
KK
D000328:Adult; D000818:Animals; D003078:Colchicine / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005696:Galactosidases / Q000302:isolation & purification*; D005959:Glucosidases / Q000302:isolation & purification*; D006801:Humans; D007106:Immune Sera; D007223:Infant; D007422:Intestines / Q000201:enzymology*; D007700:Kinetics; D010694:Lactase-Phlorizin Hydrolase / Q000037:antagonists & inhibitors / Q000276:immunology / Q000302:isolation & purification*; D008871:Microvilli / Q000201:enzymology; D011233:Precipitin Tests; D011817:Rabbits; D051381:Rats; D001616:beta-Galactosidase / Q000037:antagonists & inhibitors / Q000276:immunology / Q000302:isolation & purification*
D007106:Immune Sera; D005696:Galactosidases; D005959:Glucosidases; D001616:beta-Galactosidase; D010694:Lactase-Phlorizin Hydrolase; D003078:Colchicine