Suppression of diamine oxidase activity enhances postresection ileal proliferation in the rat.
To assess the influence of diamine oxidase activity on the adaptive process of the small bowel after resection, we administered aminoguanidine, a potent diamine oxidase inhibitor, to rats for 10 days after either small bowel transection (n = 5) or 80% jejunoileal resection (n = 7). Five or more additional animals from each group received saline as controls. Ileal mucosal homogenates from the resection group receiving aminoguanidine, when compared with those from resection controls, showed no diamine oxidase activity with increased putrescine content and ornithine decarboxylase activity. Mucosal proliferation, as measured by mucosal mass, protein content, and deoxyribonucleic acid content, was greater in the resected animals receiving aminoguanidine when compared with that of resection controls. Sucrase activity per gram of mucosa was almost identical in both resection groups. These results show that the suppression of diamine oxidase during the postresection adaptive period results in enhanced mucosal proliferation with no effect on mucosal functional differentiation. Diamine oxidase may play a regulatory role in adaptive intestinal proliferation.
Erdman
S H
SH
Department of Pediatrics, University of Nebraska School of Medicine, Omaha.
Park
J H
JH
Thompson
J S
JS
Grandjean
C J
CJ
Hart
M H
MH
Vanderhoof
J A
JA
D000222:Adaptation, Physiological; D006631:Amine Oxidase (Copper-Containing) / Q000037:antagonists & inhibitors / Q000502:physiology*; D000818:Animals; D004247:DNA / Q000378:metabolism; D006146:Guanidines / Q000494:pharmacology; D007082:Ileum / Q000201:enzymology / Q000473:pathology / Q000601:surgery*; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000201:enzymology* / Q000473:pathology; D007583:Jejunum / Q000201:enzymology / Q000473:pathology / Q000601:surgery*; D008297:Male; D011184:Postoperative Period; D011700:Putrescine / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013095:Spermidine / Q000378:metabolism; D013393:Sucrase / Q000378:metabolism
D006146:Guanidines; D004247:DNA; D006631:Amine Oxidase (Copper-Containing); D013393:Sucrase; C004479:pimagedine; D013095:Spermidine; D011700:Putrescine