Properties of ShB A-type potassium channels expressed in Shaker mutant Drosophila by germline transformation.
We have used P element-mediated germline transformation to express ShB channels in Shaker mutant Drosophila and have examined their properties by patch-clamp of embryonic myotubes. The transformed ShB cDNA was placed under the transcriptional control of a heat shock promoter (hsp70). Northern blots revealed that transformed DNA is efficiently transcribed in response to heat shock. Cultured myotubes from the transformants produced large A-type potassium currents in response to heat shock. Although qualitatively similar to native Shaker A-currents in wild-type myotubes, transformant A-current inactivates more rapidly and recovers from inactivation more rapidly, similar to ShB channels expressed in Xenopus oocytes. Unlike the channels in oocytes, however, the transformant A-current is insensitive to 50 nM charybdotoxin.
Zagotta
W N
WN
Department of Neurobiology, Stanford University School of Medicine, California 94305-5401.
Germeraad
S
S
Garber
S S
SS
Hoshi
T
T
Aldrich
R W
RW
D000818:Animals; D018999:Charybdotoxin; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004330:Drosophila / Q000196:embryology / Q000235:genetics* / Q000378:metabolism; D006358:Hot Temperature; D009132:Muscles / Q000378:metabolism; D009154:Mutation; D015221:Potassium Channels / Q000187:drug effects / Q000502:physiology*; D012604:Scorpion Venoms / Q000494:pharmacology; D012769:Shock; D014170:Transformation, Genetic
D004251:DNA Transposable Elements; D015221:Potassium Channels; D012604:Scorpion Venoms; D018999:Charybdotoxin