title: Anti-interferon-gamma antibodies in sera from HIV infected patients.
abstract: High serum levels of antibodies to interferon-gamma (IFN-gamma) have been found in patients infected with human immunodeficiency virus (HIV). A radioimmunoassay (RIA) with a recombinant IFN-gamma protein or an affinity purified IFN-gamma preparation as antigens, was developed to detect the specific anti-IFN-gamma antibodies. Reactivity of sera to IFN-gamma was confirmed by Western blot analysis. These antibodies, however, do not seem to recognize the active site of the molecule, since they do not neutralize the antiviral IFN-gamma activity in a biological assay. These results enforce the hypothesis of the role of autoimmunization during the course of the disease.
authors.affiliation: Institute of Microbiology, University of Brescia, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Caruso
authors.forename: I
authors.initials: I
authors.lastname: Foresti
authors.forename: G
authors.initials: G
authors.lastname: Gribaudo
authors.forename: C
authors.initials: C
authors.lastname: Bonfanti
authors.forename: P
authors.initials: P
authors.lastname: Pollara
authors.forename: A
authors.initials: A
authors.lastname: Dolei
authors.forename: S
authors.initials: S
authors.lastname: Landolfo
authors.forename: A
authors.initials: A
authors.lastname: Turano
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology*; D000328:Adult; D001323:Autoantibodies / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology; D015153:Blotting, Western; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007371:Interferon-gamma / Q000276:immunology*; D011863:Radioimmunoassay
chemical_list: D001323:Autoantibodies; D007074:Immunoglobulin G; D007371:Interferon-gamma