title: Lysine biosynthesis in Penicillium chrysogenum is regulated by feedback inhibition of alpha-aminoadipate reductase.
abstract: A partially purified preparation of alpha-aminoadipate reductase (EC 1.2.1.31) from Penicillium chrysogenum is competitively inhibited by lysine (Ki of 0.26 mM). Exogenous addition of 10 mM L-lysine to resting mycelia of P. chrysogenum increased the intracellular lysine pool concentration 2-fold, but decreased the incorporation of (6-14C)-alpha-aminoadipate into protein-bound lysine to a fifth. The distribution of radioactivity in the pathway metabolites alpha-aminoadipate, saccharopine and lysine was consistent with the assumption of a lysine sensitive enzyme step in vivo between alpha-aminoadipate and saccharopine. Hence lysine inhibition of alpha-aminoadipate reductase may be of physiologic importance.
authors.affiliation: Department of Microbial Biochemistry, Vienna Technical University, Austria.
authors.forename: K
authors.initials: K
authors.lastname: Affenzeller
authors.forename: W M
authors.initials: WM
authors.lastname: Jaklitsch
authors.forename: C
authors.initials: C
authors.lastname: Hönlinger
authors.forename: C P
authors.initials: CP
authors.lastname: Kubicek
mesh_terms: D015074:2-Aminoadipic Acid / Q000378:metabolism; D000445:Aldehyde Oxidoreductases / Q000037:antagonists & inhibitors*; D005246:Feedback; D050647:L-Aminoadipate-Semialdehyde Dehydrogenase; D008239:Lysine / Q000096:biosynthesis* / Q000494:pharmacology; D010407:Penicillium / Q000378:metabolism*; D010408:Penicillium chrysogenum / Q000187:drug effects / Q000378:metabolism*
chemical_list: D015074:2-Aminoadipic Acid; D000445:Aldehyde Oxidoreductases; D050647:L-Aminoadipate-Semialdehyde Dehydrogenase; D008239:Lysine