Isolation of the rat s-myc gene having tumor suppressing activity.
We have isolated a myc related gene, called s-myc, from a rat genomic library using a 32P-labeled fragment of exon 3 of v-myc as a probe. Nucleotide sequencing showed that the s-myc gene contains an open reading frame of 1,287 bp without introns. The s-Myc protein predicted from the DNA sequence has approximately 60% amino acid sequence homology with that of the mouse N-Myc protein. Transfection of the s-myc gene into rat RT4-AC tumor cells induced morphological changes of the cells and suppressed their tumorigenicity in nude mice.
Kuchino
Y
Y
National Cancer Center Research Institute, Tokyo, Japan.
Sugiyama
A
A
Asami
Y
Y
Kume
A
A
Lee
S Y
SY
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D016147:Genes, Tumor Suppressor; D016259:Genes, myc; D051379:Mice; D008819:Mice, Nude; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D051381:Rats / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000502:physiology; D012689:Sequence Homology, Nucleic Acid; D014407:Tumor Cells, Cultured / Q000473:pathology / Q000637:transplantation
D011993:Recombinant Fusion Proteins