title: Eosinophilia, granuloma formation, migratory behaviour of second stage larvae in murine Toxocara canis infection. Effect of the inoculum size.
abstract: Experimental infection of mice with Toxocara canis provides one of the best models for immunological and pathological studies of the visceral larva migrans syndrome. Blood eosinophilia, the migratory behaviour of second stage larvae and granuloma formation were studied in Swiss mice infected with Toxocara canis. Eosinophilia, spleen, liver and lung indexes were followed during a primary infection with different inoculum sizes (500 and 1500 eggs) while the migratory behaviour of larvae was studied in a primary infection with 1500 eggs over a period of 4 months. In mice infected with three challenges of 1500 eggs in order to elicit a strong inflammatory reaction in the tissues, a histopathological study was carried out. The results showed that eosinophilia, spleen and lung indexes (but not the liver index) were influenced by the parasite inoculum size. The migratory behaviour study showed that larval recovery was maximal three days post-infection, from the liver and lungs; the peak recovery from the skeletal muscles and brain being on days 15 and 30 post-infection, respectively. The histopathological study revealed the formation of granulomas in all the tissues examined (liver, lungs, kidneys, spleen, lymph nodes, myocardium etc.) but not in nervous tissue or in the retina of the eye. Granulomas in the lungs were larger than those found in the liver. The implications of these results are discussed considering host-parasite inter-relations.
authors.affiliation: Istituto di Parassitologia, Facoltà di Medicina Veterinaria, Perugia.
authors.forename: D
authors.initials: D
authors.lastname: Piergili Fioretti
authors.forename: A
authors.initials: A
authors.lastname: Moretti
authors.forename: L
authors.initials: L
authors.lastname: Mughetti
authors.forename: F
authors.initials: F
authors.lastname: Bruschi
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004802:Eosinophilia / Q000209:etiology*; D006099:Granuloma / Q000209:etiology*; D007814:Larva / Q000302:isolation & purification / Q000502:physiology; D007816:Larva Migrans, Visceral / Q000473:pathology*; D008099:Liver / Q000469:parasitology; D008168:Lung / Q000469:parasitology; D008297:Male; D051379:Mice; D013154:Spleen / Q000469:parasitology; D014119:Toxocara / Q000254:growth & development* / Q000302:isolation & purification; D014120:Toxocariasis / Q000150:complications* / Q000469:parasitology