title: [Introduction of King A and King B media for the diagnosis of Pseudomonas aeruginosa at the Teaching Pediatric Hospital of Centro Habana].
abstract: The use of King A and King B media for the faster diagnosis of Pseudomonas aeruginosa is introduced in the Microbiology Laboratory of Centro Habana Pediatric Teaching Hospital. The appropriate use of these media allows to classify as Pseudomonas aeruginosa 77.2% of the Pseudomonas sp. strains studied, which represent 95.2% of the total of strains identified as Pseudomonas aeruginosa, according to Hugh and Gilardi conventional technique. There are no significant differences in the implementation of the two procedures (X2: 0.5; p less than 0.05). The demonstrations of pyocines with King A medium is significantly superior to the method for extraction and solubility of the pigment in chloroform (X2: 15.05; p less than 0.01).
authors.forename: M F
authors.initials: MF
authors.lastname: Pérez Monrás
authors.forename: L E
authors.initials: LE
authors.lastname: Azahares Romero
authors.forename: D P
authors.initials: DP
authors.lastname: Rodríguez González
authors.forename: J L
authors.initials: JL
authors.lastname: Zuazo Silva
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods; D003462:Cuba; D003470:Culture Media; D006776:Hospitals, Pediatric; D006784:Hospitals, Teaching; D006801:Humans; D009842:Oligopeptides; D010860:Pigments, Biological / Q000032:analysis / Q000096:biosynthesis; D011549:Pseudomonas / Q000145:classification; D011552:Pseudomonas Infections / Q000175:diagnosis*; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification* / Q000378:metabolism; D011710:Pyocyanine / Q000032:analysis / Q000096:biosynthesis
chemical_list: D003470:Culture Media; D009842:Oligopeptides; D010860:Pigments, Biological; C042453:pyoverdin; D011710:Pyocyanine
vernacular_title: Introducción de los medios King A y King B para el diagnóstico de Pseudomonas aeruginosa en el Hospital Pediátrico Docente de Centro Habana.