Cloning and sequencing of the peroxisomal amine oxidase gene from Hansenula polymorpha.
We have cloned the AMO gene, encoding the microbody matrix enzyme amine oxidase (EC 1.4.3.6) from the yeast Hansenula polymorpha. The gene was isolated by differential screening of a cDNA library, immunoselection, and subsequent screening of a H. polymorpha genomic library. The nucleotide sequence of a 3.6 kilobase stretch of DNA containing the amine oxidase (AMO) gene was determined. The AMO gene contains an open reading frame of 692 amino acids, with a relative molecular mass of 77,435. The 5' and 3' ends of the gene were mapped and show that the transcribed region measures 2134 nucleotides. The derived amino-acid sequence was confirmed by sequencing an internal proteolytic fragment of the purified protein. Amine oxidase contains the tripeptide sequence Ser-Arg-Leu, located 9 residues from the carboxy terminus, which may represent the topogenic signal for protein import into microbodies.
Bruinenberg
P G
PG
Laboratory of Biochemistry, Groningen University, The Netherlands.
Evers
M
M
Waterham
H R
HR
Kuipers
J
J
Arnberg
A C
AC
AB
G
G
D006631:Amine Oxidase (Copper-Containing) / Q000235:genetics*; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D003062:Codon; D004271:DNA, Fungal / Q000648:ultrastructure; D005800:Genes, Fungal; D008830:Microbodies / Q000201:enzymology; D008969:Molecular Sequence Data; D010843:Pichia / Q000235:genetics* / Q000648:ultrastructure; D021382:Protein Sorting Signals / Q000235:genetics; D004718:Saccharomycetales / Q000235:genetics*
D000596:Amino Acids; D003062:Codon; D004271:DNA, Fungal; D021382:Protein Sorting Signals; D006631:Amine Oxidase (Copper-Containing)