title: The heat shock response of type 1 and type 4 gonococci.
abstract: A heat shock response, characterized by the elevated expression of certain proteins in response to a shift to a higher temperature, has been observed in both eukaryotic and prokaryotic organisms. We have characterized the heat shock response of the pathogenic organism N. gonorrhoeae, colony types 1 and 4. Following a shift up in temperature from 37 C to 43 C, two-dimensional gel electrophoretic analysis was used to identify 19 heat shock proteins in Type 1 and 37 heat shock proteins in Type 4 gonococci. One heat shock protein was found only in Type 1, 19 were common to both Type 1 and Type 4, and 19 were found only in Type 4 gonococci. Most heat shock proteins were found in the cytoplasm, some in the cytoplasmic membrane, and none could be detected in the outer membrane. Pulse-chase experiments revealed that heat shock proteins were very stable.
authors.affiliation: Department of Microbiology and Immunology, University of Rochester, NY 14642.
authors.forename: K W
authors.initials: KW
authors.lastname: Klimpel
authors.forename: V L
authors.initials: VL
authors.lastname: Clark
mesh_terms: D001345:Autoradiography; D002458:Cell Fractionation; D003593:Cytoplasm / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D000431:Ethanol / Q000494:pharmacology; D006360:Heat-Shock Proteins / Q000032:analysis* / Q000096:biosynthesis; D006358:Hot Temperature; D009344:Neisseria gonorrhoeae / Q000032:analysis* / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D006360:Heat-Shock Proteins; D000431:Ethanol