title: Collagen lattice effects on fibroblast arachidonic acid metabolism.
abstract: Fibroblasts are routinely maintained in vitro on tissue culture plastic, in an environment which is devoid of collagen, the most abundant extracellular protein in dermis. Recent work has shown that by seeding fibroblasts into a collagen matrix, many aspects of their metabolism change dramatically: they stop proliferation, organize and contract the collagen matrix, and secrete much larger quantities of the usual extracellular matrix components. Because so many fibroblast functions are dramatically altered by the presence of the collagen matrix, matrix effects on fibroblast metabolism of arachidonic acid were examined. The studies presented here show that during the period of matrix contraction, metabolism of arachidonate to prostaglandins by fibroblasts is increased sixfold compared to cells plated on plastic, and that this increase is correlated with contraction but does not regulate it. The increase in prostaglandin synthesis is due in part to an increased new synthesis of the rate-limiting enzyme in prostaglandin synthesis, cyclooxygenase. No change in the profile of products the fibroblasts synthesize from arachidonate is induced by the presence of the matrix. After the lattice contraction is complete, the basal arachidonate metabolism of matrix-embedded cells have the same capacity to synthesize PGE2 in response to IL-1 as do cells grown on plastic. However, the response to the hormone agonist bradykinin by the matrix-embedded cells is present on day 1 but not on day 3, the time when cells grown on plastic are most responsive. These data indicate that while basal prostaglandin metabolism is unaffected in quiescent fibroblasts which have been embedded in a collagen matrix, response to hormone agonists may be greatly attenuated. The changes in the metabolism of arachidonate which occur during the process of matrix contraction and organization may play a part in the regulation of wound repair.
authors.affiliation: Division of Dermatology, Washington University School of Medicine, St. Louis, Missouri 63110.
authors.forename: A P
authors.initials: AP
authors.lastname: Pentland
mesh_terms: D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D002452:Cell Count; D002478:Cells, Cultured; D003094:Collagen / Q000502:physiology; D015232:Dinoprostone / Q000096:biosynthesis*; D005109:Extracellular Matrix / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D012867:Skin / Q000166:cytology
chemical_list: D001095:Arachidonic Acids; D016718:Arachidonic Acid; D003094:Collagen; D015232:Dinoprostone