Analysis of HLA B27 in ankylosing spondylitis with human alloreactive cytolytic T lymphocyte clones: failure to detect disease-related T cell epitopes.
We have generated several human alloreactive cytolytic T lymphocyte (CTL) clones specific for HLA B27 expressed on cells of normals or of patients with ankylosing spondylitis (AS). These clonal T cell reagents were used to test the recognition of panels of target cells from B27+AS+, B27+AS- and B27- individuals. None of these CTL clones distinguished differences between B27+AS+ and B27+AS- cells. Three clones recognized subtypes of HLA B27 and two of these were cytolytic with group-reactive epitopes of other HLA antigens. The results suggest that there are no immunogenic disease-specific epitopes of HLA B27 in B27-linked spondyloarthropathy.
Czitrom
A A
AA
Department of Surgery, University of Toronoto, Ont., Canada.
Pototschnik
R
R
Edwards
S
S
Gladman
D D
DD
Falk
J A
JA
D002999:Clone Cells / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D015796:HLA-B27 Antigen / Q000145:classification / Q000276:immunology*; D006801:Humans; D007959:Lymphocyte Culture Test, Mixed; D013167:Spondylitis, Ankylosing / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000939:Epitopes; D015796:HLA-B27 Antigen