Glycoconjugates in normal wound tissue matrices during the initiation phase of limb regeneration in adult Ambystoma.
The present study identifies, localizes, and reports the relative composition of specific glycosaminoglycans within tissue matrices during the initiation phase of limb regeneration. The regenerate tissues were harvested and assayed morphologically, histochemically, and chemically. We observed 1) a population of cells interspersed among the cells of the dermis, epimysium, perimysium, perichondrium, and periosteum. 2) This population was distinguishable by a unique pattern of glycoconjugate staining, i.e., intracellular and pericellular heparan sulfate and glycoproteins and extracellularly associated hyaluronate and glycoproteins. 3) Cells with these staining characteristics aggregated to a position directly beneath the apical epidermal cap. 4) Extracellular hyaluronate and glycoproteins colocalized with undifferentiated tissues. And 5) extracellular chondroitin sulfate, dermatan sulfate, and keratan sulfate glycosaminoglycans colocalized with differentiated tissues. The correlations of distinct glycoconjugate compositions with specific regeneration morphologies suggest the possibility that these components may be related to the phenotypic expression of tissues during regeneration.
Young
H E
HE
Department of Anatomy, Texas Tech University Health Sciences Center, Lubbock 79430.
Dalley
B K
BK
Markwald
R R
RR
D000557:Ambystoma / Q000502:physiology*; D000818:Animals; D002809:Chondroitin Sulfates / Q000032:analysis; D003871:Dermatan Sulfate / Q000032:analysis; D000078404:Epidermal Cells; D004817:Epidermis / Q000502:physiology; D005121:Extremities / Q000032:analysis / Q000293:injuries / Q000502:physiology*; D006001:Glycoconjugates / Q000032:analysis*; D006023:Glycoproteins / Q000032:analysis; D006820:Hyaluronic Acid / Q000032:analysis; D007632:Keratan Sulfate / Q000032:analysis; D008832:Microchemistry; D010641:Phenotype; D012038:Regeneration; D013053:Spectrophotometry / Q000379:methods
D006001:Glycoconjugates; D006023:Glycoproteins; D003871:Dermatan Sulfate; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates; D007632:Keratan Sulfate