title: Amino acid sequence of mouse nidogen, a multidomain basement membrane protein with binding activity for laminin, collagen IV and cells.
abstract: The whole amino acid sequence of nidogen was deduced from cDNA clones isolated from expression libraries and confirmed to approximately 50% by Edman degradation of peptides. The protein consists of some 1217 amino acid residues and a 28-residue signal peptide. The data support a previously proposed dumb-bell model of nidogen by demonstrating a large N-terminal globular domain (641 residues), five EGF-like repeats constituting the rod-like domain (248 residues) and a smaller C-terminal globule (328 residues). Two more EGF-like repeats interrupt the N-terminal and terminate the C-terminal sequences. Weak sequence homologies (25%) were detected between some regions of nidogen, the LDL receptor, thyroglobulin and the EGF precursor. Nidogen contains two consensus sequences for tyrosine sulfation and for asparagine beta-hydroxylation, two N-linked carbohydrate acceptor sites and, within one of the EGF-like repeats an Arg-Gly-Asp sequence. The latter was shown to be functional in cell attachment to nidogen. Binding sites for laminin and collagen IV are present on the C-terminal globule but not yet precisely localized.
authors.affiliation: Max-Planck-Institut für Biochemie, Martinsried, FRG.
authors.forename: K
authors.initials: K
authors.lastname: Mann
authors.forename: R
authors.initials: R
authors.lastname: Deutzmann
authors.forename: M
authors.initials: M
authors.lastname: Aumailley
authors.forename: R
authors.initials: R
authors.lastname: Timpl
authors.forename: L
authors.initials: L
authors.lastname: Raimondi
authors.forename: Y
authors.initials: Y
authors.lastname: Yamada
authors.forename: T C
authors.initials: TC
authors.lastname: Pan
authors.forename: D
authors.initials: D
authors.lastname: Conway
authors.forename: M L
authors.initials: ML
authors.lastname: Chu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001485:Basement Membrane / Q000378:metabolism; D001665:Binding Sites; D003094:Collagen / Q000378:metabolism; D004247:DNA / Q000235:genetics; D004815:Epidermal Growth Factor / Q000235:genetics; D007797:Laminin / Q000378:metabolism; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid
chemical_list: D007797:Laminin; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D009363:Neoplasm Proteins; C029967:nidogen; D004815:Epidermal Growth Factor; D003094:Collagen; D004247:DNA