Short-term desensitization of phosphatidylinositol turnover via muscarinic acetylcholine receptors and histamine H1-receptors in smooth muscle.
Smooth muscle of guinea-pig taenia caecum was desensitized by treatment with 10(-4)M carbachol or 10(-4)M histamine for 30 min in Ca-free solution containing 2mM EGTA. Phosphatidylinositol turnover stimulated by carbachol was not reduced by desensitization with either carbachol or histamine, while the turnover stimulated by histamine was reduced by desensitization with histamine, but not with carbachol. These results are consistent with our previous report (1) that heterologous desensitization induced by carbachol occurs at intracellular Ca stores and homologous desensitization by histamine occurs at H1 receptors.
Hishinuma
S
S
Department of Molecular Pharmacology, Meiji College of Pharmacy, Tokyo, Japan.
Uchida
M K
MK
D000818:Animals; D001285:Atropine / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D003106:Colon; D004533:Egtazic Acid / Q000494:pharmacology; D005260:Female; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology; D007295:Inositol Phosphates / Q000378:metabolism; D008297:Male; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000378:metabolism*; D011738:Pyrilamine / Q000494:pharmacology; D011969:Receptors, Histamine H1 / Q000502:physiology*; D011976:Receptors, Muscarinic / Q000502:physiology*
D007295:Inositol Phosphates; D010716:Phosphatidylinositols; D011969:Receptors, Histamine H1; D011976:Receptors, Muscarinic; D004533:Egtazic Acid; D001285:Atropine; D006632:Histamine; D002217:Carbachol; D011738:Pyrilamine