title: The genetics of catalase in Drosophila melanogaster: isolation and characterization of acatalasemic mutants.
abstract: Activated oxygen species have been demonstrated to be the important agents in oxygen toxicity by disrupting the structural and functional integrity of cells through lipid peroxidation events, DNA damage and protein inactivation. The biological consequences of free radical damage have long been hypothesized to be a causal agent in many aging-related diseases. Catalase (H2O2:H2O2 oxidoreductase; EC 1.15.1.1) is one of several enzymes involved in the scavenging of oxygen free radicals and free radical derivatives. The structural gene for catalase in Drosophila melanogaster has been localized to region 75D1-76A on chromosome 3L by dosage responses to segmental aneuploidy. This study reports the isolation of a stable deficiency, Df(3L)CatDH104(75C1-2;75F1), that uncovers the catalase locus and the subsequent isolation of six acatalasemic mutants. All catalase mutants are viable under standard culture conditions and recessive lethal mutations within the 75Cl-F1 interval have been shown not to affect catalase activity. Two catalase mutations are amorphic while four are hypomorphic alleles of the Cat+ locus. The lack of intergenic complementation between the six catalase mutations strongly suggests that there is only one functional gene in Drosophila. One acatalesemic mutation was mapped to position 3-47.0 which resides within the catalase dosage sensitive region. While complete loss of catalase activity confers a severe viability effect, residual levels are sufficient to restore viability to wild type levels. These results suggest a threshold effect for viability and offer an explanation for the general lack of phenotypic effects associated with the known mammalian acatalasemics.
authors.affiliation: Department of Genetics, North Carolina State University, Raleigh 27695-7614.
authors.forename: W J
authors.initials: WJ
authors.lastname: Mackay
authors.forename: G C
authors.initials: GC
authors.lastname: Bewley
mesh_terms: D020642:Acatalasia; D000483:Alleles; D000818:Animals; D002374:Catalase / Q000235:genetics*; D002874:Chromosome Mapping; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D005609:Free Radicals; D006861:Hydrogen Peroxide / Q000633:toxicity; D009154:Mutation; D010100:Oxygen / Q000378:metabolism; D011995:Recombination, Genetic
chemical_list: D005609:Free Radicals; D006861:Hydrogen Peroxide; D002374:Catalase; D010100:Oxygen