title: Calcium phosphate-mediated transfection alters metallothionein gene expression in response to Cd2+ and Zn2+.
abstract: The level of expression of a transfected metallothionein (MT)-IGcat fusion gene in response to cadmium differed from that of the endogenous MT-IG gene. Atomic absorption analysis indicated that the total cellular content of cadmium and zinc increased upon calcium phosphate-mediated transfection. Thus, changes in the influx/efflux of metals may regulate the level of MT gene expression.
authors.affiliation: Department of Biological Sciences, University of Calgary, Alberta, Canada.
authors.forename: R
authors.initials: R
authors.lastname: Foster
authors.forename: P E
authors.initials: PE
authors.lastname: Olsson
authors.forename: L
authors.initials: L
authors.lastname: Gedamu
mesh_terms: D002104:Cadmium / Q000378:metabolism; D000001:Calcimycin / Q000494:pharmacology; D002130:Calcium Phosphates / Q000494:pharmacology; D002460:Cell Line; D003001:Cloning, Molecular; D003300:Copper / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D008668:Metallothionein / Q000235:genetics*; D014162:Transfection / Q000187:drug effects; D015032:Zinc / Q000378:metabolism
chemical_list: D002130:Calcium Phosphates; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; D002104:Cadmium; D000001:Calcimycin; C485838:calcium phosphate, monobasic, anhydrous; D003300:Copper; D008668:Metallothionein; C020243:calcium phosphate; D015032:Zinc; C485829:calcium phosphate, dibasic, anhydrous