Detection of tetracycline-resistant strains of Ureaplasma urealyticum by hybridization assays.
Five wild-type strains of tetracycline-resistant Ureaplasma Ureaplasma urealyticum and one tetracycline-sensitive Ureaplasma urealyticum strain were tested for presence of the tetM sequence by hybridization assays. Two sorts of probes were used, a plasmid containing the tetM fragment and the tetM fragment alone. Two sorts of label were used for each, digoxigenin and radio-labelling. Both purified DNA at various concentrations and non-purified DNA in the pellet from a 10 ml culture were used as targets. The digoxigenin-labelled whole plasmid probe was as specific as the insert alone when hybridized with non-purified DNA, and was more sensitive than radio-labelled probes.
Brunet
B
B
Laboratoire de Bacteriologie, Hopital Pellegrin, Bordeaux, France.
de Barbeyrac
B
B
Renaudin
H
H
Bébéar
C
C
D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D013292:Streptococcus agalactiae / Q000187:drug effects; D013753:Tetracycline Resistance / Q000235:genetics*; D014509:Ureaplasma / Q000187:drug effects* / Q000235:genetics
D015342:DNA Probes; D004269:DNA, Bacterial