The human lymphocyte micronucleus assay: a comparison between whole-blood and separated-lymphocyte cultures.
The induction of micronuclei (MN) by vincristine, mitomycin C and cyclophosphamide was compared in purified lymphocytes and in whole-blood cultures. With both assays, cytokinesis was blocked by cytochalasin B and MN were only scored in binucleate cells. The data suggest that whole-blood cultures may be considered a better experimental condition for the detection of MN induced by chemicals in vitro.
Migliore
L
L
Dipartimento di Scienze dell'Ambiente e del Territorio, Università di Pisa, Italy.
Nieri
M
M
Amodio
S
S
Loprieno
N
N
D001773:Blood Cells; D002469:Cell Separation; D002478:Cells, Cultured; D003520:Cyclophosphamide / Q000633:toxicity; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects*; D015162:Micronucleus Tests; D016685:Mitomycin; D008937:Mitomycins / Q000633:toxicity; D009153:Mutagens; D014750:Vincristine / Q000633:toxicity
D008937:Mitomycins; D009153:Mutagens; D016685:Mitomycin; D014750:Vincristine; D003520:Cyclophosphamide