The G protein alpha o subunit alters morphology, growth kinetics, and phospholipid metabolism of somatic cells.
The physiological role of the alpha o subunit of guanine nucleotide-binding (G) protein was investigated with a murine adrenal cell line (Y1) transfected with a rat alpha o cDNA cloned in a retroviral expression vector. The parental cell line lacked detectable alpha o subunit. Expression of the alpha o cDNA in transfected cell lines was confirmed by Western blot (immunoblot) analysis. The rat alpha o subunit interacted with murine beta and gamma subunits and associated with cell membranes. Y1 cells containing large amounts of alpha o subunit had altered cellular morphology and reduced rate of cell division. In addition, GTP-gamma S-stimulated release of arachidonic acid from these cells was significantly increased compared with that in control cells. The alpha o subunit appears directly or indirectly to regulate cellular proliferation, morphology, and phospholipid metabolism.
Bloch
D B
DB
Department of Genetics, Massachusetts General Hospital, Boston 02114.
Bonventre
J V
JV
Neer
E J
EJ
Seidman
J G
JG
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism; D002455:Cell Division; D002460:Cell Line; D003001:Cloning, Molecular; D019204:GTP-Binding Proteins / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D005796:Genes; D005822:Genetic Vectors; D007700:Kinetics; D046911:Macromolecular Substances; D051379:Mice; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D014162:Transfection
D001095:Arachidonic Acids; D046911:Macromolecular Substances; D010743:Phospholipids; D019204:GTP-Binding Proteins