[Islands of Langerhans of cattle--potential donor tissue for clinical islet transplantation?].
A standard method for bovine islet isolation has been developed. The mean islet yield per gram pancreas obtained from the last twelve consecutive glands was 2,098 +/- 292 representing an insulin recovery of 36.1 +/- 6.5%. The static incubation of freshly isolated bovine islets in the presence of glucose resulted in a less distinct increase of insulin secretion than in the presence of free fatty acids. Nevertheless, xenotransplantation of bovine islets to immunosuppressed C57BL6J mice did reverse streptozotocin-induced diabetes for 2 to 6 days.
Hering
B J
BJ
Medizinischen Klinik III und Poliklinik, Justus-Liebig-Universität Giessen.
Romann
D
D
Clarius
A
A
Brendel
M
M
Bretzel
R G
RG
Federlin
K
K
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002417:Cattle; D003921:Diabetes Mellitus, Experimental / Q000601:surgery*; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D016381:Islets of Langerhans Transplantation; D051379:Mice; D008810:Mice, Inbred C57BL; D014021:Tissue Preservation / Q000379:methods; D014183:Transplantation, Heterologous
D001786:Blood Glucose; D007328:Insulin
Langerhans' sche Inseln des Rindes--potentielles Spendergewebe für die klinische Inseltransplantation?