Investigations on in vitro bone resorbing activity from athymic (nude) and euthymic mouse splenic leukocytes.
T-lymphocyte dependence of the production of in vitro bone resorbing activity was examined using athymic (nu/nu) and euthymic (nu/+) mouse splenic leukocytes. Conditioned medium (CM) from unstimulated splenic leukocytes of nu/nu mice had greater in vitro bone resorbing activity compared to CM from nu/+ mice (1.7- as compared to 1.2-fold increase of 45Ca release in mouse calvaria). CM from concanavalin A (Con A)-treated nu/nu and nu/+ leukocytes had 1.8-fold and no increase in 45Ca release, respectively. CM from both nu/nu and nu/+ phytohemagglutinin (PHA)-treated leukocytes had a 1.7-fold increase in 45Ca release. Bone resorbing activity from nu/nu CM was inhibited by interferon-tau (10 & 100 IU/mL) and indomethacin (2 x 10(-6) M). CM (untreated or Con A-treated) from nu/nu leukocytes had higher levels of prostaglandin E (PGE) as compared to CM from nu/+ leukocytes, and indomethacin decreased PGE levels in nu/nu CM. Leukocytes from nu/+ mice had increased mitogenesis when stimulated with PHA (1, 3, & 10 micrograms/mL) or Con A (1 and 10 micrograms/mL), whereas leukocytes from nu/nu mice were nonresponsive or had significant inhibition of mitogenesis with PHA and Con A.(ABSTRACT TRUNCATED AT 250 WORDS)
McCauley
L K
LK
Department of Veterinary Pathobiology, Ohio State University, Columbus 43210.
Rosol
T J
TJ
Capen
C C
CC
Horton
J E
JE
Shanfeld
J
J
D000818:Animals; D000831:Animals, Newborn; D001862:Bone Resorption / Q000276:immunology*; D003208:Concanavalin A / Q000494:pharmacology; D003470:Culture Media; D046508:Culture Techniques; D007213:Indomethacin / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology; D008213:Lymphocyte Activation; D051379:Mice; D008819:Mice, Nude; D010835:Phytohemagglutinins / Q000494:pharmacology; D011458:Prostaglandins E / Q000032:analysis; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D003470:Culture Media; D010835:Phytohemagglutinins; D011458:Prostaglandins E; D003208:Concanavalin A; D007371:Interferon-gamma; D007213:Indomethacin