title: [Use of immunospecific methods for quantitative estimation of microsomal epoxide hydrolase].
abstract: Antiserum, specific to rat liver microsomal epoxide hydrolase, was produced. Three methods for quantitative estimation of the epoxide hydrolase, base on the principle of antigen-antiserum immunoprecipitation, were used: electroimmune analysis by Laurell, radial immunodiffusion, enzyme-linked immunoassay. Distinct correlation between specific enzyme activity and its content in microsomal preparation was demonstrated using a simple and stable procedure of radial immunodiffusion. In the enzyme-linked assay conjugate of protein A with alkaline phosphate served as the secondary antibody. Sensitivity of the procedure described was about 1,000-fold higher as compared with radial immunodiffusion method.
authors.forename: V L
authors.initials: VL
authors.lastname: D'iakov
authors.forename: A V
authors.initials: AV
authors.lastname: Dubovskoĭ
authors.forename: O A
authors.initials: OA
authors.lastname: Klimova
authors.forename: V A
authors.initials: VA
authors.lastname: Sukhanov
mesh_terms: D000818:Animals; D002138:Calibration; D004797:Enzyme-Linked Immunosorbent Assay; D004851:Epoxide Hydrolases / Q000378:metabolism*; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D008862:Microsomes, Liver / Q000201:enzymology*; D011817:Rabbits; D051381:Rats
chemical_list: D004851:Epoxide Hydrolases
vernacular_title: Ispol'zovanie immunospetsificheskikh metodov dlia opredeleniia kolichestva mikrosomal'noĭ époksidgidrolazy.