Recruitment and activation of tumor-specific immune T cells in situ. CD8+ cells predominate the secondary response in sponge matrices and exert both delayed-type hypersensitivity-like and cytotoxic T lymphocyte activity.
This study analyzes the involvement of CD4+ and CD8+ T cells in a secondary cellular immune response to the highly metastatic murine lymphoma ESb in situ. This tumor line expresses tumor-associated transplantation Ag which can induce protective immunity in vivo and specific CTL in vitro. In tumor-immune mice the injection of a tumor vaccine (x-irradiated ESb tumor cells) into s.c. implanted vascularized sponges resulted in the generation of a specific secondary immune response characterized by massive leukocyte recruitment and generation of strong CTL activity at the restimulation site. During the antitumor immune response the CD4+:CD8+ T cell ratio decreased significantly and specifically in the restimulated sponges. Depletion of CD8+ but not CD4+ T cells from the tumor immune mice before restimulation significantly reduced the delayed-type hypersensitivity-like response and totally blocked the generation of tumor-specific CTL activity in situ. Only a minority of the CD8+ immune T cells which predominated the secondary response in situ expressed IL-2R and lymph node homing receptors as detected by the mAb MEL-14.
Zangemeister-Wittke
U
U
Institute for Immunology and Genetics, German Cancer Research Center, Heidelberg.
Kyewski
B
B
Schirrmacher
V
V
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D016827:CD8 Antigens; D002465:Cell Movement; D003602:Cytotoxicity, Immunologic; D005109:Extracellular Matrix / Q000276:immunology; D005260:Female; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007117:Immunization, Secondary; D007940:Leukemia L5178 / Q000276:immunology* / Q000378:metabolism; D007942:Leukemia, Experimental / Q000276:immunology*; D008198:Lymph Nodes; D008213:Lymphocyte Activation; D051379:Mice; D008811:Mice, Inbred DBA; D010641:Phenotype; D011140:Polyurethanes; D011971:Receptors, Immunologic / Q000378:metabolism; D015375:Receptors, Interleukin-2 / Q000378:metabolism; D016201:Receptors, Lymphocyte Homing; D013154:Spleen; D013602:T-Lymphocytes, Cytotoxic / Q000145:classification / Q000276:immunology* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D016827:CD8 Antigens; D011140:Polyurethanes; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2; D016201:Receptors, Lymphocyte Homing