Inhibition of trout gill and soybean lipoxygenases by organotin compounds.
Activity of trout gill and soybean lipoxygenase was inhibited by various low concentrations of tri-n-butyltin, di-n-butyltin and n-butyltin chlorides at pH 7.8. Tri-n-butyltin (0.044-0.19 mM; 8-100% inhibition) was significantly more inhibitory against trout gill lipoxygenase than di-n-butyltin (0.1-0.27 mM; 17-39% inhibition) or n-butyltin (1.2-3.6 mM; 26-43% inhibition). Soybean lipoxygenase displayed a similar sensitivity to the inhibitory effects of organotin compounds at pH 7.8. Although tri-n-butyltin (0.067-0.2 mM; 13-68% inhibition) was less inhibitory to soybean lipoxygenase, di-n-butyltin (0.134-0.537 mM; 51-75% inhibition) and n-butyltin (0.41-1.23 mM; 33-75% inhibition) were more inhibitory towards soybean lipoxygenase compared with trout gill lipoxygenase. Concentrations of butyltins required to provide substantial inhibition of trout gill lipoxygenase were of the order of magnitude of those reported for lethal water exposure concentrations of these compounds for trout and other fishes when bioaccumulation factors are considered. Thus, it is proposed that inhibition of lipoxygenase likely contributes to the biocidal activity of organotin compounds.
Josephson
D B
DB
Department of Food Science, University of Wisconsin-Madison 53706.
Lindsay
R C
RC
Stuiber
D A
DA
D000818:Animals; D005880:Gills / Q000187:drug effects / Q000201:enzymology; D008084:Lipoxygenase / Q000302:isolation & purification; D016859:Lipoxygenase Inhibitors; D009947:Organotin Compounds / Q000494:pharmacology*; D012487:Salmonidae / Q000378:metabolism*; D013025:Glycine max / Q000201:enzymology*; D013045:Species Specificity; D014220:Trialkyltin Compounds / Q000494:pharmacology; D014337:Trout / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000633:toxicity
D016859:Lipoxygenase Inhibitors; D009947:Organotin Compounds; D014220:Trialkyltin Compounds; D014874:Water Pollutants, Chemical; C056189:di-n-butyltin; C062120:n-butyltin; C011559:tributyltin; D008084:Lipoxygenase; C020735:dibutyldichlorotin