title: Central giant cell granulomas of the jaws. Nuclear DNA analysis using image cytometry.
abstract: Giant cell nuclear DNA, in 30 giant cell lesions of the jaws, was quantified by computer-assisted image analysis. DNA content was then used to predict clinical behavior and outcome. 4 nuclei in each of 25 giant cells (total = 100 nuclei) were randomly selected and the DNA content was quantified by the Leitz Texture-Analysis-System-Plus. DNA in nuclei of normal appearing stromal fibroblasts (n = 20) was similarly measured. The DNA index was calculated as the mean nuclear DNA content of giant cells divided by the mean DNA content of control fibroblasts. The mean DNA-index of aggressive lesions (1.09, SD = 0.12) was not significantly different from that of non-aggressive lesions (1.18, SD = 0.15) (p = 0.093). The results indicate that the nuclear DNA content of giant cells is not useful as a predictor of the clinical behavior of giant cell lesions of the jaws.
authors.affiliation: Department of Oral and Maxillofacial Surgery, University of California San Francisco, School of Dentistry.
authors.forename: A
authors.initials: A
authors.lastname: Eckardt
authors.forename: M A
authors.initials: MA
authors.lastname: Pogrel
authors.forename: L B
authors.initials: LB
authors.lastname: Kaban
authors.forename: K
authors.initials: K
authors.lastname: Chew
authors.forename: B H
authors.initials: BH
authors.lastname: Mayall
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002467:Cell Nucleus / Q000648:ultrastructure*; D002648:Child; D004247:DNA / Q000032:analysis / Q000648:ultrastructure*; D004171:Diploidy; D005260:Female; D006101:Granuloma, Giant Cell / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007571:Jaw Diseases / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged
chemical_list: D004247:DNA