Comparative detoxification and protection of scorpion (Heterometrus bengalensis) venom by toxoid antiserum.
Comparative detoxification of scorpion venom by using different chemical agents was investigated. Detoxification by formalin showed the best optimum detoxifying agent. The formalin treatment resulted in 2.3% protein loss with 6-fold detoxification. This formal toxoid was immunogenic in rabbit giving high neutralizing antibodies as revealed from indirect haemagglutination test. Toxoid antiserum protected mice against the lethal action of venom. It also effectively antagonized the smooth muscle contractile response of venom, and venom-induced neuromuscular paralysis. This toxoid antiserum also protected the venom-induced cardiac arrest. The possibility of using this formal toxoid for antisera production and immunization for therapeutic use needs to be explored.
Das Gupta
S C
SC
Gomes
A
A
Gomes
A
A
Basu
A
A
Lahiri
S C
SC
D000818:Animals; D000997:Antivenins / Q000494:pharmacology; D005557:Formaldehyde; D006168:Guinea Pigs; D051379:Mice; D009119:Muscle Contraction; D009200:Myocardial Contraction; D051381:Rats; D012604:Scorpion Venoms / Q000037:antagonists & inhibitors / Q000276:immunology / Q000633:toxicity*; D012605:Scorpions; D013552:Swine; D008063:Thioctic Acid / Q000031:analogs & derivatives; D014121:Toxoids / Q000276:immunology
D000997:Antivenins; D012604:Scorpion Venoms; D014121:Toxoids; D005557:Formaldehyde; D008063:Thioctic Acid; C015144:dihydrolipoic acid