Detection of beta-galactofuranosidase production by Penicillium and Aspergillus species using 4-nitrophenyl beta-D-galactofuranoside.
An assay was developed for detecting beta-galactofuranosidase produced by Penicillium and Aspergillus spp. The substrate for the assay, 4-nitrophenyl beta-D-galactofuranoside, was synthesized from penta-O-acetyl-beta-D-galactofuranose and 4-nitrophenol by a tin chloride catalyzed reaction followed by O-deacetylation. Aspergillus spp. produced only small quantities of beta-galactofuranosidase during 30 d at 25 degrees C. Only the biverticillate Penicillium spp. (P. funiculosum, P. islandicum, P. rubrum and P. tardum) produced substantial beta-galactofuranosidase after 1-4 weeks at 25 degrees C. No extracellular antigens of these four Penicillium spp. could be detected in culture filtrates by the sandwich ELISA technique when antibodies to the extracellular beta-galactofuranoside-containing polysaccharide antigen of P. digitatum was used. Antigens to all other Penicillium and Aspergillus spp. were easily detected in their culture filtrates.
Cousin
M A
MA
Purdue University, Department of Food Science, West Lafayette, Indiana 47907.
Notermans
S
S
Hoogerhout
P
P
Van Boom
J H
JH
D000946:Antigens, Fungal / Q000032:analysis; D001230:Aspergillus / Q000201:enzymology* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005696:Galactosidases / Q000032:analysis*; D005697:Galactosides; D006026:Glycoside Hydrolases; D010407:Penicillium / Q000201:enzymology* / Q000276:immunology; D001616:beta-Galactosidase / Q000032:analysis* / Q000096:biosynthesis
D000946:Antigens, Fungal; D005697:Galactosides; C060549:4-nitrophenylgalactofuranoside; D005696:Galactosidases; D006026:Glycoside Hydrolases; C023112:exo-beta-D-galactofuranosidase; D001616:beta-Galactosidase