Rescue of bicoid mutant Drosophila embryos by bicoid fusion proteins containing heterologous activating sequences.
The maternal gene bicoid (bcd) determines pattern in the anterior half of the Drosophila embryo. It is reported here that the injection of bcd mutant embryos with messenger RNAs that encode proteins consisting of heterologous acidic transcriptional activating sequences fused to the DNA-binding portion of the bcd gene product, can completely restore the anterior pattern of the embryo.
Driever
W
W
Max-Planck-Institut fur Entwicklungsbiologie, Abteilung Genetik, Tubingen, FRG.
Ma
J
J
Nüsslein-Volhard
C
C
Ptashne
M
M
D000818:Animals; D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins / Q000235:genetics*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000196:embryology*; D005786:Gene Expression Regulation; D005796:Genes; D018398:Homeodomain Proteins; D007301:Insect Hormones; D008845:Microinjections; D009024:Morphogenesis; D012333:RNA, Messenger / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D013329:Structure-Activity Relationship; D015534:Trans-Activators / Q000235:genetics*
D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D018398:Homeodomain Proteins; D007301:Insect Hormones; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; C056478:bcd protein, Drosophila