The immunomodulatory effect of CCA (N-(2-carboxyphenyl)-4-chloroanthranilic acid disodium salt) on IgG antibody response in mice immunized with ovalbumin.
CCA(N-(2-carboxyphenyl)-4-chloroanthranilic acid disodium salt is a new synthetic compound which structurally resembles mefenamic acid. CCA induces inhibition of the adjuvant arthritis in rats and has been reported as activator of suppressor T lymphocytes. These findings stimulated to us to elucidate whether or not CCA possesses inhibitory effects on immunoglobulin E (IgE) production, an immunological system in which T suppressor cells play a cardinal role. Mice F1 (BALB/c x C57BL/6J) and C3H/A were immunized using the procedure of several s.c. injections with low dose of ovalbumin and aluminium hydroxide gel. CCA (5 mg/kg and 50 mg/kg) was administered to mice several weeks by oral route. The titers of IgE were compared in controls and treated animals using the method of passive cutaneous anaphylaxis (PCA) in rats. At a dose of 5 mg/kg CCA reduced partially the titers of IgE in treated animals with respect to controls and at a dose of 50 mg/kg this inhibition was more pronounced. These results support a potential usefulness of CCA in bronchial asthma and other immediate type allergic disorders.
Gonzalez
R
R
National Center for Scientific Research (CENIC), Department of Pharmacology and Toxicology, Havana, Cuba.
Wyczółkowska
J
J
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005260:Female; D007114:Immunization; D007143:Immunoglobulin Heavy Chains / Q000276:immunology*; D007139:Immunoglobulin epsilon-Chains / Q000276:immunology*; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000276:immunology*; D062367:ortho-Aminobenzoates / Q000494:pharmacology*
D007143:Immunoglobulin Heavy Chains; D007139:Immunoglobulin epsilon-Chains; D007166:Immunosuppressive Agents; D062367:ortho-Aminobenzoates; D010047:Ovalbumin; C015735:lobenzarit