Stable indicator cell lines exhibiting HIV-1 tat function.
Since HIV tat function is essential for the HIV infectious cycle, it represents an important possible target of therapeutic intervention for HIV infection. Stable human cell lines were derived that express high levels of beta-galactosidase under the combined control of the transacting HIV-1 tat gene product and the cis-acting HIV-1 LTR. The tat gene product induces LTR-linked gene expression approximately 1000-fold in this system. The high level of expression of beta-galactosidase under HIV tat and LTR control in stable cell lines allows rapid spectrophotometric quantitation of beta-galactosidase enzymatic activity from fewer than 5000 cells seeded in a microtiter plate well. Such cell lines provide a virus-free system for the high-capacity screening of compounds for the ability to interfere with HIV tat-mediated transactivation of gene expression.
Bacheler
L T
LT
Medical Products Department, E.I. du Pont de Nemours & Co., Inc., Wilmington, DE 19880-0400.
Strehl
L L
LL
Neubauer
R H
RH
Petteway
S R
SR
Ferguson
B Q
BQ
D002460:Cell Line; D005786:Gene Expression Regulation; D015696:Gene Products, tat; D005814:Genes, Viral; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D014779:Virus Replication; D001616:beta-Galactosidase / Q000235:genetics; D054322:tat Gene Products, Human Immunodeficiency Virus
D015696:Gene Products, tat; D014157:Transcription Factors; D054322:tat Gene Products, Human Immunodeficiency Virus; D001616:beta-Galactosidase