[Heterotransplantability of human anaplastic thyroid carcinoma line (HOTHC) in nude mice, and biological properties of the heterograft].
The heterotransplantability of HOTHC line (human anaplastic thyroid carcinoma) into the subcutis of BALB/c nude mice and the biological properties of grafts were discussed. (1) The HOTHC line showed high transplantability, and 1 x 10(4) cells produced anaplastic carcinoma (giant cell type) containing the colloid-like substance. (2) The grafted tumors grew rapidly and the mice were dead within 2 months after transplantation. (3) The number of leukocytes (neutrophils) of mouse peripheral blood increased as the tumor size increased, and the leukocyte count returned to a normal value after removal of the tumor. (4) The conditioned media of HOTHC line formed colonies of granulocytes (neutrophils) on soft agar. These phenomena revealed that HOTHC is a granulocyte-colony stimulating factor-producing line. (5) The conditioned media of HOTHC line showed promoting-effect on neovascularization on the chorioallantoic membrane.
Ono
I
I
Mutual Aid Association, Suifu Hospital, Japan.
Ishiwata
I
I
Nakaguchi
T
T
Konoe
K
K
Tokita
N
N
Soma
M
M
Ishiwata
C
C
Ishikawa
H
H
D000368:Aged; D000369:Aged, 80 and over; D043925:Angiogenesis Inducing Agents / Q000378:metabolism; D000818:Animals; D002277:Carcinoma / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D002455:Cell Division; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D006801:Humans; D007958:Leukocyte Count; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D013964:Thyroid Neoplasms / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
D043925:Angiogenesis Inducing Agents; D016178:Granulocyte-Macrophage Colony-Stimulating Factor