Generation of nitric oxide by human neutrophils.
Human neutrophils were evaluated for their ability to generate nitric oxide. Neutrophils incubated with superoxide dismutase at 37 degrees C produce nitrite anion at a rate of 1.8 nmols/2 x 10(6) cells/30 min, providing indirect evidence of nitric oxide production. Incubation of the neutrophils with concentrations of serum-opsonized zymosan, N-formyl-methionyl-leucyl-phenylalanine, or phorbol myristate acetate sufficient to stimulate the respiratory burst and lysosomal enzyme release caused no additional nitrite anion production. Glass wool-adherent neutrophils exhibited a similar dissociation of nitrite anion production from the respiratory burst and lysosomal enzyme release. Direct evidence for nitric oxide production was also obtained using nitric oxide-specific chemiluminescence. These results demonstrate that human neutrophils are capable of generating nitric oxide.
Wright
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CD
Pharmacology Department, Warner-Lambert Company, Ann Arbor, Michigan 48105.
Mülsch
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A
Busse
R
R
Osswald
H
H
D000838:Anions; D002448:Cell Adhesion; D005609:Free Radicals; D005898:Glass; D006801:Humans; D009113:Muramidase / Q000378:metabolism; D009504:Neutrophils / Q000201:enzymology / Q000378:metabolism* / Q000502:physiology; D009569:Nitric Oxide / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
D000838:Anions; D005609:Free Radicals; D009573:Nitrites; D013481:Superoxides; D009569:Nitric Oxide; D009113:Muramidase