Fc gamma receptor recognition of IgG ligand by human monocytes and macrophages.
We examined the binding characteristics of human monocytes and macrophages with the IgG ligands, human monomeric IgG and a small human IgG aggregate, trimeric IgG. Our purpose was to utilize fresh monocytes, in vitro cultured monocytes, and alveolar macrophages in direct and indirect binding experiments. Freshly isolated monocytes expressed only a single binding site for IgG monomer and IgG trimer. In contrast, in vitro cultured monocytes, gamma-interferon-treated monocytes, and freshly isolated alveolar macrophages expressed a single binding site for IgG monomer and, in addition, a high and low affinity binding site for IgG trimer. The high affinity binding site for IgG trimer (Kd approximately equal to 1 nM) appeared identical to the binding site for IgG monomer. The low affinity binding site for IgG trimer (Kd = 50 to 250 nM) appeared to be due to Fc gamma RII, because antibody to Fc gamma RII inhibited its expression. Since Fc gamma RII, in contrast to Fc gamma RI, does not bind monomeric IgG, the data suggest that this low affinity receptor for trimeric IgG, Fc gamma RII, can bind low molecular weight circulating immune complexes at concentrations 10- to 100-fold lower than Fc gamma RI. Thus, these studies suggest that at 37 degrees C, macrophage Fc gamma RII may play a functional role in the recognition of small molecular weight immune complexes.
Rossman
M D
MD
Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia.
Chen
E
E
Chien
P
P
Rottem
M
M
Cprek
A
A
Schreiber
A D
AD
D000943:Antigens, Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000494:pharmacology; D008024:Ligands; D008264:Macrophages / Q000502:physiology*; D009000:Monocytes / Q000502:physiology*; D011650:Pulmonary Alveoli / Q000166:cytology; D011961:Receptors, Fc / Q000502:physiology*; D017452:Receptors, IgG; D011994:Recombinant Proteins; D013997:Time Factors
D000943:Antigens, Differentiation; D007074:Immunoglobulin G; D008024:Ligands; D011961:Receptors, Fc; D017452:Receptors, IgG; D011994:Recombinant Proteins; C044411:polymeric IgG; D007371:Interferon-gamma