Recombinant bovine interferon-gamma enhances expression of class I and class II bovine lymphocyte antigens.
Recombinant bovine interferon-gamma augments expression of class I and class II histocompatibility antigens on the surface membrane of bovine lymphocytes. Immunofluorescence techniques using a series of monoclonal anti-HLA antibodies demonstrate that this enhancement is detectable as early as 24 h after incubation with rBoIFN, while maximum surface expression is obtained within 3-5 days. A concentration as low as 10 units of rBoIFN is effective. Such results may be useful for characterizing the BoLA gene products.
Walrand
F
F
INRA, Ecole Nationale Vétérinaire, Alfort, France.
Picard
F
F
McCullough
K
K
Martinod
S
S
Lévy
D
D
D000818:Animals; D000954:Antigens, Surface / Q000096:biosynthesis* / Q000276:immunology; D002417:Cattle; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis*; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis*; D007371:Interferon-gamma / Q000494:pharmacology*; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D009000:Monocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D011994:Recombinant Proteins
D000954:Antigens, Surface; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D011994:Recombinant Proteins; D007371:Interferon-gamma