Polyoma T-antigen-derived synthetic peptides induce polyoma-virus-specific macrophage migration inhibition.
Synthetic peptides, 2 derived from the sequence common to small, middle and large T-antigen, and I derived from the sequence unique for middle T, activated lymphocytes from polyoma-virus-immunized, but not from control mice, to release the lymphokine migration inhibitory factor (MIF). In contrast, purified, bacterially grown, full-length small T-antigen and a middle T-antigen mutant Py 1387T MT (lacking 37 C-terminal amino acids) did not induce lymphokine release, although they were capable of inducing an anti-polyoma TSTA response in vivo.
Reinholdsson
G
G
Department of Tumor Biology, Karolinska Institutet, Stockholm, Sweden.
Ramqvist
T
T
Szigeti
R
R
Dalianis
T
T
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000276:immunology* / Q000302:isolation & purification; D002464:Cell Migration Inhibition; D007114:Immunization / Q000379:methods; D007115:Immunization Schedule; D008262:Macrophage Activation / Q000187:drug effects; D008263:Macrophage Migration-Inhibitory Factors / Q000096:biosynthesis; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D008808:Mice, Inbred CBA; D010455:Peptides / Q000138:chemical synthesis / Q000494:pharmacology*; D011120:Polyomavirus / Q000276:immunology*
D000952:Antigens, Polyomavirus Transforming; D008263:Macrophage Migration-Inhibitory Factors; D010455:Peptides