Enhanced expression of the Epstein-Barr virus latent membrane protein by a recombinant vaccinia virus.
The complete coding sequence of the Epstein-Barr virus strain B95-8 latent membrane protein (LMP) was cloned using a Raji cell cDNA library and genomic B95-8 DNA. The clone was characterized by sequencing and then used to make a recombinant vaccinia virus. This virus (VLMP) was shown to express a relatively high level of LMP in an authentic fashion. Antisera raised in rabbits against VLMP were shown to react with B95-8 LMP as well as cross-reacting with a 50K cellular protein.
Stewart
J P
JP
Paterson Institute for Cancer Research, Manchester, U.K.
Hampson
I N
IN
Heinrich
H W
HW
Mackett
M
M
Arrand
J R
JR
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000956:Antigens, Viral / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D003001:Cloning, Molecular; D003429:Cross Reactions; D004247:DNA / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D005786:Gene Expression Regulation; D005814:Genes, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008969:Molecular Sequence Data; D011817:Rabbits; D011995:Recombination, Genetic; D014407:Tumor Cells, Cultured; D014616:Vaccinia virus / Q000235:genetics* / Q000276:immunology; D014763:Viral Matrix Proteins
D000914:Antibodies, Viral; D000956:Antigens, Viral; D004279:DNA, Viral; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins; D004247:DNA