Secretion of transforming growth factor-beta by human myelogenous leukemic cells and its possible role in proliferation of the leukemic cells.
Transforming growth factor (TGF)-beta activity was found in the neutral extracts of human myelogenous leukemic cells or K562 cells and the conditioned medium from K562 cell culture. BALB/c 3T3 cells grown in soft agar in the presence of TGF-beta 1 produced an activity that stimulated the growth of K562 cells. This activity was non-dialyzable, acid-stable, heat-sensitive and partially inactivated by pronase treatment. These results suggest a mutual growth reliance between the leukemic cells and fibroblasts mediated by paracrine growth factors produced by these cells.
Komatsu
K
K
Department of Tumor Biochemistry, Center for Adult Diseases, Osaka.
Nakamura
H
H
Shinkai
K
K
Akedo
H
H
D000818:Animals; D002455:Cell Division; D003470:Culture Media; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D007951:Leukemia, Myeloid / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D015291:Transforming Growth Factors / Q000378:metabolism* / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D003470:Culture Media; D015291:Transforming Growth Factors