Y1 and Y2 receptors for neuropeptide Y.
By using monoiodinated radioligands of both intact neuropeptide Y (NPY) and of a long C-terminal fragment, NPY13-36, two subtypes of binding sites, which differ in affinity and specificity, have been characterized. The Y1 type of binding site, characterized on a human neuroblastoma cell line, MC-IXC, and a rat pheochromocytoma cell line, PC-12, binds NPY with a dissociation constant (Kd) of a few nanomolar but does not bind NPY13-36. The Y2 type of binding site, characterized on porcine hippocampal membranes and on another human neuroblastoma cell line, SMS-MSN, is of higher affinity and binds both NPY and NPY13-36. None of the binding sites distinguish between NPY and the homologous peptide YY (PYY). It is concluded that NPY/PYY-binding sites occur in two subtypes which may represent two types of physiological receptors.
Sheikh
S P
SP
Laboratory for Molecular Endocrinology, Rigshospitalet, Copenhagen, Denmark.
Håkanson
R
R
Schwartz
T W
TW
D000310:Adrenal Gland Neoplasms; D000818:Animals; D001665:Binding Sites; D002462:Cell Membrane / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism; D006801:Humans; D009447:Neuroblastoma; D009478:Neuropeptide Y / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism*; D010673:Pheochromocytoma; D051381:Rats; D017476:Receptors, Neuropeptide Y; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D013552:Swine; D014407:Tumor Cells, Cultured
D009478:Neuropeptide Y; D010446:Peptide Fragments; D017476:Receptors, Neuropeptide Y; D017981:Receptors, Neurotransmitter