Transient elevation of locus coeruleus alpha 2-adrenergic receptor binding during the early stages of amygdala kindling.
Enhancement of noradrenergic neurotransmission retards, but does not prevent, the development of kindling. The firing activity of noradrenergic locus coeruleus (LC) neurons is partially regulated by axon collateral recurrent inhibition mediated via alpha 2-adrenergic receptors. We tested the hypothesis that LC autoinhibitory alpha 2-adrenergic receptors may change during the kindling process thereby altering LC excitability. Specific binding of the alpha 2-adrenergic receptor antagonist [3H]RX781094 (idazoxan) was measured in the LC of rats at 3 different stages of kindling development using in vitro neurotransmitter receptor autoradiography techniques. Specific [3H]RX781094 binding was elevated significantly in rats kindled to two Class 1 kindled motor seizures. No differences in binding were observed in animals kindled to Class 3 or Class 5 kindled motor seizures. Saturation of binding experiments indicated that the increase in binding following two Class 1 kindled motor seizures was due to an increase in the total number of alpha 2-receptors without a change in the affinity of the binding site for [3H]RX781094. The transient increase in number of LC alpha 2-adrenergic receptors is consistent with the idea that noradrenergic neurotransmission inhibits the early progress of kindling development, but then subsequently becomes ineffective in maintaining the inhibition during later stages of kindling development.
Jimenez-Rivera
C A
CA
Department of Physiology, University of New Mexico School of Medicine, Albuquerque 87131.
Chen
M J
MJ
Vigil
A
A
Savage
D D
DD
Weiss
G K
GK
D000679:Amygdala / Q000503:physiopathology*; D000818:Animals; D004146:Dioxanes / Q000378:metabolism; D019329:Idazoxan; D007696:Kindling, Neurologic; D008125:Locus Coeruleus / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011942:Receptors, Adrenergic, alpha / Q000378:metabolism*
D004146:Dioxanes; D011942:Receptors, Adrenergic, alpha; D019329:Idazoxan