Subcellular localization of proteins encoded by the phenotypically cryptic plasmid of Neisseria gonorrhoeae: biological evidence for outer membrane association of the cppB gene product.
Almost all clinical isolates of Neisseria gonorrhoeae harbour a plasmid of 4.2 kb with no known function. A genetic model based on the DNA sequence of the plasmid, with ten open reading frames, has been proposed by Korch et al., (1985). To address the question of the function of the encoded proteins, some of which are expressed when the plasmid is harboured by Escherichia coli, the subcellular locations of such proteins were investigated in minicells of Escherichia coli DS410. The protein CppB, earlier proposed to be a membrane-spanning polypeptide, was found associated with the outer membrane. Up to five other cryptic plasmid proteins were found to be localized in the periplasm.
Aalen
R B
RB
Department of Biology, University of Oslo, Norway.
Lossius
I
I
Gundersen
W B
WB
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D002462:Cell Membrane / Q000032:analysis*; D002499:Centrifugation, Density Gradient; D002874:Chromosome Mapping; D006651:Histocytochemistry; D009344:Neisseria gonorrhoeae / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*
D001425:Bacterial Outer Membrane Proteins