Production of monoclonal antibodies to dehydroepiandrosterone-sulphate after immunization of mouse with dehydroepiandrosterone-bovine serum albumin conjugate.
Monoclonal antibodies with a much higher specificity for DHA-S than for DHA were obtained from a BALB/c mouse immunized with a non-sulphated DHA-7CMO-BSA antigen. An improved fusion technique using PEG containing 10% DMSO instead of PEG alone increased the number of positive hybridomas. One of the five monoclonal antibodies obtained, showed a high affinity for DHA-S (Ka = 10(10) M-1) and very low cross-reactions with androsterone (0.62%) and androsterone sulphate (0.83%) which made it potentially useful for direct quantitation of DHA-S in human serum.
Parvaz
P
P
Laboratoire de Biochimie, Hôpital Ste. Eugènie, Centre Hospitalier, Lyon-Sud, Pierre-Bénite, France.
Mathian
B
B
Patricot
M C
MC
Garcia
I
I
Revol
A
A
Mappus
E
E
Grenot
C
C
Cuilleron
C Y
CY
D000818:Animals; D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D002459:Cell Fusion; D006224:Cricetinae; D003429:Cross Reactions; D003687:Dehydroepiandrosterone / Q000031:analogs & derivatives* / Q000032:analysis / Q000276:immunology*; D019314:Dehydroepiandrosterone Sulfate; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D011863:Radioimmunoassay; D012710:Serum Albumin, Bovine
D000911:Antibodies, Monoclonal; D012710:Serum Albumin, Bovine; D003687:Dehydroepiandrosterone; D019314:Dehydroepiandrosterone Sulfate