title: Positive identification of NS4A, the last of the hypothetical nonstructural proteins of flaviviruses.
abstract: A radiolabeled protein migrating in SDS-polyacrylamide gels near the core protein C of Kunjin virus-infected cells was isolated and subjected to N-terminal amino acid sequencing. Comparisons with the translation sequence deduced from the known nucleotide sequence identified a hydrophobic protein of 149 amino acids located in the polyprotein sequence between NS3 and NS4B, thus establishing its identity as NS4A with a calculated Mr 16,100. The cleavage sites identified at the N- and C-termini are KR decreases S....parallel....VAA decreases, both representing consensus sequences defined previously for Kunjin and other flaviviruses.
authors.affiliation: Department of Microbiology, Monash University, Clayton, Victoria, Australia.
authors.forename: G
authors.initials: G
authors.lastname: Speight
authors.forename: E G
authors.initials: EG
authors.lastname: Westaway
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002213:Capsid / Q000032:analysis*; D004279:DNA, Viral / Q000235:genetics; D005416:Flavivirus / Q000032:analysis* / Q000235:genetics; D005814:Genes, Viral; D008969:Molecular Sequence Data; D008970:Molecular Weight; D014709:Vero Cells; D014758:Viral Core Proteins / Q000032:analysis*; D017361:Viral Nonstructural Proteins
chemical_list: D004279:DNA, Viral; D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins