Comparative studies on microsomal NADPH-cytochrome P-450 reductase using a monoclonal antibody: tissue distribution, specific activity and peptide mapping.
1. In order to elucidate the molecular structure and the distribution of the enzyme in different microsomes, specific antibodies have been developed against rabbit liver NADPH-cytochrome P-450 reductase. 2. The monoclonal antibody (MAb B1) against rabbit liver reductase cross-reacted well with reductases from various animal species and those from various tissues of the rabbit. 3. NADPH-cytochrome P-450 reductase from rabbit tissues such as liver, lung, adrenal gland, kidney and polymorphonuclear leukocyte were closely related in structure and antigenic properties, in addition to having similar catalytic properties. 4. No multiple forms of the reductase in the rabbit were observed in liver nor in other tissues.
Katagiri
M
M
Department of Chemistry, Osaka Kyoiku University, Tennoji-ku, Japan.
Sugiyama
T
T
Tsutsukawa
N
N
Ishiguro
H
H
Miyoshi
N
N
Ishibashi
F
F
Taniguchi
N
N
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D000918:Antibody Specificity; D015153:Blotting, Western; D003902:Detergents; D008862:Microsomes, Liver / Q000201:enzymology*; D008970:Molecular Weight; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism*; D010449:Peptide Mapping; D011817:Rabbits; D012995:Solubility
D000911:Antibodies, Monoclonal; D003902:Detergents; D009251:NADPH-Ferrihemoprotein Reductase