title: The distribution of glycosyl-phosphatidylinositol anchored proteins is differentially regulated by serum and insulin.
abstract: Glycosyl-phosphatidylinositol (GPI) anchored proteins are surveyed in two insulin sensitive cell types by surface labeling and phospholipase C-induced release into the medium. Serum starvation selectively increases both the number and intensity of a subset of GPI-anchored proteins. After serum starvation, loss of cell-surface GPI-anchored proteins is induced acutely by either serum re-exposure or insulin, suggesting that hormonal treatment may promote the release of these proteins from the cell surface.
authors.affiliation: Department of Cell Biology and Anatomy, Cornell University Medical College, New York, NY 10021.
authors.forename: M P
authors.initials: MP
authors.lastname: Lisanti
authors.forename: J C
authors.initials: JC
authors.lastname: Darnell
authors.forename: B L
authors.initials: BL
authors.lastname: Chan
authors.forename: E
authors.initials: E
authors.lastname: Rodriguez-Boulan
authors.forename: A R
authors.initials: AR
authors.lastname: Saltiel
mesh_terms: D000818:Animals; D001769:Blood; D002478:Cells, Cultured; D003470:Culture Media; D006017:Glycolipids / Q000378:metabolism*; D017261:Glycosylphosphatidylinositols; D007328:Insulin / Q000494:pharmacology; D051379:Mice; D008970:Molecular Weight; D009132:Muscles / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism*; D010738:Type C Phospholipases
chemical_list: D003470:Culture Media; D006017:Glycolipids; D017261:Glycosylphosphatidylinositols; D007328:Insulin; D010716:Phosphatidylinositols; D010738:Type C Phospholipases