Effect of recombinant soluble CD4 in rhesus monkeys infected with simian immunodeficiency virus of macaques.
The CD4 molecule is a high-affinity cell-surface receptor for the human immunodeficiency virus (HIV-1) and a soluble truncated form of CD4 produced by recombinant DNA technology is a potent inhibitor of HIV-1 replication and HIV-1-induced cell fusion in vitro. Rhesus monkeys infected with the simian immunodeficiency virus of macaques (SIVMAC), a virus closely related to HIV-1, develop an AIDS-like syndrome, and so provide an important model for the evaluation of potential AIDS therapies. We have assessed the therapeutic effect of recombinant soluble CD4 in SIVMAC-infected rhesus monkeys. Virus was readily isolated from peripheral blood lymphocytes and bone marrow cells of these animals before starting treatment with soluble CD4, but became difficult to isolate soon after treatment had begun. Moreover the diminished growth of both granulocyte-macrophage and erythrocyte progenitor colonies from the bone marrow of these monkeys rose to normal levels during treatment. These findings indicate that soluble CD4 could prove valuable in the treatment of AIDS.
Watanabe
M
M
Harvard Medical School, New England Regional Primate Research Center, Southborough, Massachusetts 01772.
Reimann
K A
KA
DeLong
P A
PA
Liu
T
T
Fisher
R A
RA
Letvin
N L
NL
D000818:Animals; D000954:Antigens, Surface / Q000008:administration & dosage / Q000276:immunology*; D001853:Bone Marrow / Q000382:microbiology / Q000473:pathology; D002478:Cells, Cultured; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000382:microbiology; D006801:Humans; D007167:Immunotherapy; D008253:Macaca mulatta; D015728:Receptors, HIV; D011991:Receptors, Virus / Q000276:immunology*; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000276:immunology* / Q000627:therapeutic use; D012192:Retroviridae Infections / Q000276:immunology / Q000628:therapy*; D015302:Simian Immunodeficiency Virus / Q000302:isolation & purification*
D000954:Antigens, Surface; D015728:Receptors, HIV; D011991:Receptors, Virus; D011994:Recombinant Proteins