[Experimental models in studying gastric secretion in dogs: innervated pouch, denervated pouch, cervical esophagostomy. Execution, monitoring, use].
Different surgical models are used in the dog for studying gastric secretion: gastric fistula, denervated Heidenhain pouch, innervated Amdrup pouch and cervical esophagostomy. The surgical procedures are described as well as the care and the monitoring allowing long-term survival of the animals. The association of gastric fistula - Heidenhain pouch has been assessed 30 times and 8 times with cervical esophagostomy. The death-rate was been 7% and the morbidity 24%. Three dogs were provided with an Amdrup pouch. Calibration of the animals allowed the acid and pepsin secreting dose-response to be platted and translation according to lineweaver-Burk or Dowd and Riggs is used to calculate the efficient dose (ED 50) and the maximal response (maxR). Pharmacological or physiological effects might be analyzed in regard to the modification of these parameters in response to a pharmacological agonist or antagonist substance. Some physiological studies could be made using sham-feeding on dogs fitted with a cervical esophagostomy.
Olivier
A
A
Riquet
W
W
Poitevin
C
C
Charleux
H
H
Mignon
M
M
Vatier
J
J
D000818:Animals; D003714:Denervation; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004946:Esophagostomy; D005750:Gastric Juice / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism*; D010434:Pepsin A / Q000378:metabolism; D013268:Stimulation, Chemical; D013270:Stomach / Q000294:innervation / Q000601:surgery
D010434:Pepsin A
Modèles expérimentaux d'étude de la sécrétion gastrique chez le chien: poche innervée, poche dénervée, oesophagostomie cervicale. Réalisation, surveillance, utilisation.