A monoclonal antibody against the slow isoform of skeletal muscle Ca2+-ATPase selectively stains a subpopulation of neurons in the central nervous system.
A monoclonal antibody against the slow isoform of chicken skeletal muscle Ca2+-ATPase recognises, in nervous tissue, analogous membrane proteins which are most concentrated in the microsomal fraction. Histochemically, the immunoreactivity of the antibody is restricted to neurones where the staining is most intense in cell bodies and dendrites, weak in axons and absent from cell nuclei. The expression of the antigen varies greatly between different neuronal populations and is developmentally regulated. The antigen is also axonally transported.
Shahin
S
S
Neuroscience Group, Faculty of Medicine, University of Newcastle, N.S.W., Australia.
Lasher
R S
RS
Millar
T J
TJ
Rostas
J A
JA
D000818:Animals; D000911:Antibodies, Monoclonal; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D002490:Central Nervous System / Q000201:enzymology*; D002645:Chickens; D007150:Immunohistochemistry; D009132:Muscles / Q000201:enzymology*; D013347:Subcellular Fractions / Q000201:enzymology
D000911:Antibodies, Monoclonal; D000252:Calcium-Transporting ATPases