Absence of alpha-2 adrenergic effects on cAMP production in a genital tract smooth muscle cell line.
This study sought to evaluate alpha-2 and beta adrenergic modulation of cAMP production in the DDT1 MF-2 transformed smooth muscle myocyte. After stimulation with forskolin or adrenergic agonists with or without subtype specific antagonists, cAMP production was determined. These experiments confirmed an increase of cAMP in response to forskolin, isoproterenol, epinephrine, and norepinephrine; the adrenergic stimulation was inhibited by propranolol. On the other hand, the alpha-2 agonist clonidine did not inhibit cAMP production. Likewise, alpha-2 receptor blockade did not increase cAMP production in response to epinephrine. These studies, therefore, suggest that the DDT1 MF-2 myocyte does not contain a significant population of functional alpha-2 adrenergic receptors.
Phillippe
M
M
Department of Obstetrics and Gynecology, Michael Reese Hospital, Chicago, Illinois.
Saunders
T
T
Hariharan
S
S
D000818:Animals; D002461:Cell Line, Transformed; D003000:Clonidine / Q000494:pharmacology; D005576:Colforsin / Q000494:pharmacology; D006224:Cricetinae; D000242:Cyclic AMP / Q000096:biosynthesis*; D004837:Epinephrine / Q000494:pharmacology; D005834:Genital Neoplasms, Male; D007545:Isoproterenol / Q000494:pharmacology; D007890:Leiomyosarcoma; D008297:Male; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism*; D009638:Norepinephrine / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D011942:Receptors, Adrenergic, alpha / Q000502:physiology*; D014407:Tumor Cells, Cultured; D014649:Vas Deferens
D011942:Receptors, Adrenergic, alpha; D005576:Colforsin; D011433:Propranolol; D000242:Cyclic AMP; D007545:Isoproterenol; D003000:Clonidine; D009638:Norepinephrine; D004837:Epinephrine