Integrity of the exon 6 sequence is essential for tissue-specific alternative splicing of human leukocyte common antigen pre-mRNA.
By alternative splicing, exons 4, 5, and 6 of the human leukocyte common antigen (LCA) gene are included in B-cell mRNA but excluded from thymocyte mRNA. A mini-LCA gene that contains only LCA exons 2, 6, and 8 faithfully reproduces this tissue-specific alternative splicing in mouse B and thymocyte cell lines. Elimination of almost all of the intron sequences associated with exon 6 had no effect on the alternative splicing, while linker-scanning analysis showed that a significant length of the exon 6 sequence is essential for alternative splicing.
Tsai
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AY
Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, Massachusetts.
Streuli
M
M
Saito
H
H
D000943:Antigens, Differentiation / Q000235:genetics*; D001402:B-Lymphocytes / Q000032:analysis; D001483:Base Sequence; D004252:DNA Mutational Analysis; D005091:Exons / Q000502:physiology*; D005822:Genetic Vectors; D006649:Histocompatibility Antigens / Q000235:genetics*; D006801:Humans; D007438:Introns / Q000502:physiology; D017493:Leukocyte Common Antigens; D008969:Molecular Sequence Data; D012326:RNA Splicing / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D013601:T-Lymphocytes / Q000032:analysis; D014162:Transfection
D000943:Antigens, Differentiation; D006649:Histocompatibility Antigens; D012333:RNA, Messenger; D017493:Leukocyte Common Antigens