title: Lack of correlation between levels of MHC class I antigen and susceptibility to lysis of small cellular lung carcinoma (SCLC) by natural killer cells.
abstract: Small cellular lung carcinoma (SCLC) cell lines are susceptible to lysis by NK cells. SCLC, normally negative for MHC class I Ag, were rendered positive for HLA-A and -B Ag by two methods: treatment with IFN-gamma or transfection with HLA class I genes. Exposure to IFN-gamma induced high levels of class I Ag and reduced susceptibility to NK-mediated lysis. However, transfection with either HLA-A2, HLA-B27, or HLA-B27 with beta 2m did not result in reduced susceptibility to NK cells. These transfectants expressed amounts of HLA class I Ag comparable to those in IFN-gamma-treated, untransfected cells. Transfection with the beta 2m gene or plasmid alone neither influenced levels of surface class I Ag nor resulted in reduced susceptibility to lysis by NK cells. Thus, the effects of IFN-gamma on NK susceptibility can be dissociated from the induction of class I Ag.
authors.affiliation: Department of Cellular Biochemistry, Netherlands Cancer Institute, Amsterdam.
authors.forename: N J
authors.initials: NJ
authors.lastname: Stam
authors.forename: W M
authors.initials: WM
authors.lastname: Kast
authors.forename: A C
authors.initials: AC
authors.lastname: Voordouw
authors.forename: L B
authors.initials: LB
authors.lastname: Pastoors
authors.forename: F A
authors.initials: FA
authors.lastname: van der Hoeven
authors.forename: C J
authors.initials: CJ
authors.lastname: Melief
authors.forename: H L
authors.initials: HL
authors.lastname: Ploegh
mesh_terms: D018288:Carcinoma, Small Cell / Q000235:genetics / Q000276:immunology*; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D015234:HLA-A Antigens / Q000032:analysis / Q000235:genetics / Q000276:immunology; D015235:HLA-B Antigens / Q000032:analysis / Q000235:genetics / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular; D007371:Interferon-gamma / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000276:immunology*; D008175:Lung Neoplasms / Q000235:genetics / Q000276:immunology*; D014162:Transfection; D001613:beta 2-Microglobulin / Q000032:analysis / Q000235:genetics / Q000276:immunology
chemical_list: D015234:HLA-A Antigens; D015235:HLA-B Antigens; D015395:Histocompatibility Antigens Class I; D001613:beta 2-Microglobulin; D007371:Interferon-gamma