The bovine papillomavirus type 1 transcriptional activator E2 protein binds to its DNA recognition sequence as a dimer.
The transcriptional trans-activator E2 protein from bovine papillomavirus type 1 has been shown to bind to the DNA consensus sequence ACCN6GGT. We have produced the DNA-binding domain of the E2 protein as a recombinant protein in Escherichia coli. The E2 DNA-binding domain was purified as two different molecular weight forms. Using these purified proteins, we show that two molecules of the E2 protein bind to a single DNA consensus binding site.
Moskaluk
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CA
Department of Microbiology and Immunology, Duke University Medical Center, Durham, North Carolina 27710.
Bastia
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D
D000818:Animals; D052688:Bovine papillomavirus 1 / Q000235:genetics*; D002417:Cattle; D003001:Cloning, Molecular; D015342:DNA Probes; D004279:DNA, Viral / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D004742:Enhancer Elements, Genetic; D027383:Papillomaviridae / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
D015342:DNA Probes; D004279:DNA, Viral; D004268:DNA-Binding Proteins; C051550:E2 protein, Bovine papillomavirus; D014157:Transcription Factors; D014764:Viral Proteins