Distinct chromosomal abnormalities in murine leukemia virus-induced T- and B-cell lymphomas.
We performed a cytogenetic study on 16 murine mature B-cell lymphomas and 10 T-cell lymphomas, using G-banding techniques. All tumors, with the exception of 3 spontaneous B-cell tumors, were induced by various slowly transforming murine leukemia viruses (MuLV). Metaphases were obtained from primary (10 B-cell tumors) and first or second transplant generation lymphomas (6 B-cell and 10 T-cell tumors), all of which were well characterized with respect to phenotypic, histologic and genotypic features. In the T-cell tumors we found relatively simple karyotypic abnormalities, including various numerical aberrations, such as trisomy 15, in line with many earlier reports. However, the majority of B-cell tumors showed a great variety of both structural and numerical chromosomal anomalies. Three B-cell lymphomas had an apparently normal karyotype. No single cytogenetic abnormality occurred commonly in the B-cell lymphomas, but some structural abnormalities were found in more than one stemline, in particular, ins (II) (A1; A2) in 3 tumors, and deletions involving the D-region of chromosome 14 in 3 other lymphomas. These cytogenetic results clearly indicate that the pathogenic mechanisms involved in MuLV-induced (long latency) B-cell lymphomagenesis and (short latency) T-cell lymphomagenesis differ considerably.
Vasmel
W L
WL
The Netherlands Cancer Institute, Antoni van Leeuwenhoek Huis, Amsterdam.
Matthews
E A
EA
Gillis
C P
CP
Nieland
J
J
Borst
E A
EA
Leupers
C J
CJ
Melief
C J
CJ
Slater
R M
RM
D000818:Animals; D000831:Animals, Newborn; D001402:B-Lymphocytes; D002869:Chromosome Aberrations / Q000209:etiology / Q000235:genetics*; D002871:Chromosome Banding; D025063:Chromosome Disorders; D007621:Karyotyping; D009052:Leukemia Virus, Murine; D008223:Lymphoma / Q000209:etiology / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D013601:T-Lymphocytes; D014407:Tumor Cells, Cultured