title: Sporozoite vaccine induces genetically restricted T cell elimination of malaria from hepatocytes.
abstract: The target of the CD8+ T cell-dependent immunity that protects mice immunized with irradiation-attenuated malaria sporozoites has not been established. Immune BALB/c mice were shown to develop malaria-specific, CD8+ T cell-dependent inflammatory infiltrates in their livers after challenge with Plasmodium berghei sporozoites. Spleen cells from immune BALB/c and C57BL/6 mice eliminated hepatocytes infected with the liver stage of P. berghei in vitro. The activity against infected hepatocytes is not inhibited by antibodies to interferon-gamma and is not present in culture supernatants. It is genetically restricted, an indication that malaria antigens on the hepatocyte surface are recognized by immune T effector cells. Subunit vaccine development will require identification of the antigens recognized by these T cells and a method of immunization that induces such immunity.
authors.affiliation: Infectious Diseases Department, Naval Medical Research Institute, Bethesda, MD 20814.
authors.forename: S L
authors.initials: SL
authors.lastname: Hoffman
authors.forename: D
authors.initials: D
authors.lastname: Isenbarger
authors.forename: G W
authors.initials: GW
authors.lastname: Long
authors.forename: M
authors.initials: M
authors.lastname: Sedegah
authors.forename: A
authors.initials: A
authors.lastname: Szarfman
authors.forename: L
authors.initials: L
authors.lastname: Waters
authors.forename: M R
authors.initials: MR
authors.lastname: Hollingdale
authors.forename: P H
authors.initials: PH
authors.lastname: van der Meide
authors.forename: D S
authors.initials: DS
authors.lastname: Finbloom
authors.forename: W R
authors.initials: WR
authors.lastname: Ballou
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D000913:Antibodies, Protozoan / Q000032:analysis; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology; D006183:H-2 Antigens / Q000276:immunology; D007114:Immunization; D007371:Interferon-gamma / Q000276:immunology / Q000494:pharmacology; D008099:Liver / Q000276:immunology / Q000469:parasitology*; D008288:Malaria / Q000276:immunology* / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010962:Plasmodium berghei / Q000276:immunology*; D011994:Recombinant Proteins; D013154:Spleen / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D014612:Vaccines / Q000276:immunology
chemical_list: D000906:Antibodies; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D006183:H-2 Antigens; D011994:Recombinant Proteins; D014612:Vaccines; D007371:Interferon-gamma