[Study on the metastatic mechanism and invasive degree of tumor cells after subcutaneous transplantation of uterine cervix carcinoma cells into footpad of mouse].
After mouse ascitic carcinoma cells (U14) 2 X 10(6) were inoculated subcutaneously into right footpads of inbred 615-strain mice, the subcutaneous primary tumor growth was resected by right hind leg amputation in 6-10 mice 1, 2, 3, 5, 7, 9 and 11 days after inoculation respectively. Another 9 mice without hind leg amputation served as controls. All animals were sacrificed on the 26th day. Results showed that tumor invasion was not apparent on the 1st day after transplantation (grade 0). Slight tumor invasion was noticed on the 2nd day (grade I); grades II-III invasion were noticed on the 3rd-5th days; and grades III-IV invasion on the 7-11th days. Lymphatic metastases appeared on the 7th day after transplantation, both lymphatic metastasis and lung metastases occurred in 80% of the mice 11 days after inoculation. The results indicate a close relationship between tumor cell invasion and metastasis, i.e., tumor metastasis was noticed on the 7th day as tumor invasion reached grade III. Also, lymphatic metastasis was noticed 2 days earlier than lung metastasis. Results of this experiment provide very useful data for studies on the treatment of tumor invasion and metastasis and the relationship between lymphatic and hematogenic metastasis.
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D000818:Animals; D005260:Female; D008175:Lung Neoplasms / Q000556:secondary; D008207:Lymphatic Metastasis; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D002583:Uterine Cervical Neoplasms / Q000473:pathology*