Optimal replication of plasmids carrying polyomavirus origin regions requires two high-affinity binding sites for large T antigen.
The efficiency of replication of plasmids containing the control region of polyomavirus DNA including one, two, or all three of the strong binding sites for large T antigen was measured in COP 8 cells which provide polyomavirus T antigen in trans. It was found that plasmids carrying only binding site A (the one closest to the origin core region) exhibited only 10% of the replication competence of plasmids with binding sites A and B or A and C. Plasmids containing all three binding sites, A, B, and C, did not replicate more efficiently than those with only two strong T-antigen-binding sites. We conclude, therefore, that optimal T-antigen-dependent replication of polyomavirus DNA requires two high-affinity T-antigen-binding sites.
Weichselbraun
I
I
Institute of Molecular Biology, University of Vienna, Austria.
Haider
G
G
Wintersberger
E
E
D000952:Antigens, Polyomavirus Transforming / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010957:Plasmids; D011120:Polyomavirus / Q000235:genetics*
D000952:Antigens, Polyomavirus Transforming; D004279:DNA, Viral; D004268:DNA-Binding Proteins