[Prolongation of rat hepatic allografts by donor splenocyte infusion and peritransplant cyclosporine treatment].
This study examined the effect of pretransplant infusion of donor splenocytes and peritransplant Cyclosporine (CsA) treatment on Wistar orthotopic liver allograft survival in recipient SD rats. Treatment with either donor splenocytes or CsA significantly prolonged the survival of recipients. When pretransplant infusion of donor splenocytes combined with peritransplant use of CsA, 4/7 animals survived indefinitely (greater than 150 days). T cell subsets classification in the peripheral blood of long survivors by means of McAbs (MRC W/OX) and flow cytometer were significantly different from those in controls. The percentage of W3/25+ cells was lower and that of OX-8+ cells was higher with a decreased ratio of W3/25+/OX-8+. These results suggested the important role of suppressor T-lymphocytes in the induction of specific immunologic unresponsiveness to rat hepatic allografts.
Sun
J H
JH
D000818:Animals; D003524:Cyclosporins / Q000627:therapeutic use*; D006085:Graft Survival / Q000187:drug effects; D016031:Liver Transplantation / Q000276:immunology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000166:cytology / Q000637:transplantation*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D003524:Cyclosporins