The effect of long-term consumption of fish oil on platelet-activating factor synthesis in rat renal microsomes.
The effect of consuming eicosapentaenoic acid (EPA) plus docosahexaenoic acid (DHA) on platelet-activating factor (PAF) biosynthesis by renal acetyl-CoA: 1-O-alkyl-sn-glycero-3-phosphocholine (lysoPAF) acetyltransferase activity was compared in rats fed diets containing fish oil (FO) or sunflower oil (SO) for 11 months. Microsomes from the FO group exhibited a significantly lower formation of [14C]PAF from [1-14C]acetyl-CoA in the absence of exogenously-added lysoPAF. There were no differences between the two groups in the generation of radioactive acylacetyl-glycerophosphocholine (GPC) upon incubation of microsomes without added lysoPAF. Upon addition of lysoPAF, the microsomal synthesis of PAF, but not acylacetyl-GPC, rose considerably. Furthermore, PAF synthesis via microsomal acetyltransferase activity in the FO-fed animals was only 40% that of the SO groups. These findings may bear potential relevance in terms of reducing PAF synthesis in association with renal pathophysiology.
Yeo
Y K
YK
Department of Nutritional Sciences, University of Guelph, Ontario, Canada.
Philbrick
D J
DJ
Holub
B J
BJ
D000105:Acetyl Coenzyme A / Q000378:metabolism; D000123:Acetyltransferases / Q000378:metabolism*; D000818:Animals; D004041:Dietary Fats / Q000494:pharmacology*; D004281:Docosahexaenoic Acids / Q000494:pharmacology; D015118:Eicosapentaenoic Acid / Q000494:pharmacology; D005395:Fish Oils / Q000494:pharmacology*; D005997:Glycerylphosphorylcholine / Q000096:biosynthesis; D007668:Kidney / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D008297:Male; D008861:Microsomes / Q000187:drug effects / Q000201:enzymology*; D010938:Plant Oils / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000031:analogs & derivatives / Q000096:biosynthesis* / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D000074242:Sunflower Oil
D004041:Dietary Fats; D005395:Fish Oils; C029271:O-deacetyl platelet activating factor; D010938:Plant Oils; D010972:Platelet Activating Factor; D000074242:Sunflower Oil; D004281:Docosahexaenoic Acids; D005997:Glycerylphosphorylcholine; D000105:Acetyl Coenzyme A; D015118:Eicosapentaenoic Acid; D000123:Acetyltransferases; C046035:1-alkylglycerophosphocholine acetyltransferase