title: A mechanism of c-raf-1 activation: fusion of the lipocortin II amino-terminal sequence with the c-raf-1 kinase domain.
abstract: In order to understand the mechanism of oncogenic activation, we have analyzed the c-raf-1 gene from the GL-5-JCK human glioblastoma, which underwent rearrangement during transfection experiments. Nucleotide sequencing of cDNA clones derived from the 2.5 kb raf-mRNA, which is a major transcript of raf in NIH3T3 cells transformed with GL-5-JCK DNA, revealed that this mRNA contains sequences derived from the human c-raf-1 gene and the human lipocortin II gene. Translation of the 2.5 kb raf-mRNA predicted a fusion protein consisting of 16 amino-terminal amino acid residues of the lipocortin II and 370 carboxy-terminal amino acid residues of the c-raf-1 protein which contains the kinase domain. Expression of the lipocortin II-raf cDNA using the murine sarcoma virus long terminal repeat as promoter resulted in the transformation of NIH3T3 cells.
authors.affiliation: The Institute of Medical Science, University of Tokyo, Japan.
authors.forename: F
authors.initials: F
authors.lastname: Mitsunobu
authors.forename: M
authors.initials: M
authors.lastname: Fukui
authors.forename: T
authors.initials: T
authors.lastname: Oda
authors.forename: T
authors.initials: T
authors.lastname: Yamamoto
authors.forename: K
authors.initials: K
authors.lastname: Toyoshima
mesh_terms: D017302:Annexins; D001483:Base Sequence; D002471:Cell Transformation, Neoplastic; D004247:DNA / Q000032:analysis; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D019908:Proto-Oncogene Proteins c-raf; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000032:analysis; D014162:Transfection
chemical_list: D017302:Annexins; D006023:Glycoproteins; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D004247:DNA; D019908:Proto-Oncogene Proteins c-raf