title: Vasopressin receptor distribution in adrenalectomized rats using vasopressin anti-idiotype.
abstract: Following adrenalectomy, it has been demonstrated that parvocellular corticotropin-releasing factor-containing neurons in the paraventricular nucleus (PVN) of rat hypothalamus synthesize vasopressin. The present study examined whether putative vasopressin receptors are expressed in parallel with the appearance of vasopressin immunoreactivity in these parvocellular neurons. A vasopressin anti-idiotypic antibody which immunostains putative vasopressin receptors associated with magnocellular PVN neurons was utilized. Following adrenalectomy, antivasopressin immunostained neurons in parvocellular and magnocellular PVN, whereas the anti-idiotypic antibody immunostained magnocellular neurons only. We therefore conclude that the putative vasopressin receptor recognized by the anti-idiotype is not demonstrated in association with parvocellular vasopressin-producing neurons of the adrenalectomized rat.
authors.affiliation: Neuroendocrine Unit, University of Rochester, NY 14642.
authors.forename: D J
authors.initials: DJ
authors.lastname: Berlove
authors.forename: D T
authors.initials: DT
authors.lastname: Piekut
mesh_terms: D000315:Adrenalectomy; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology; D007150:Immunohistochemistry; D008297:Male; D008473:Median Eminence / Q000033:anatomy & histology / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D010286:Paraventricular Hypothalamic Nucleus / Q000033:anatomy & histology / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011945:Receptors, Angiotensin / Q000378:metabolism*; D017483:Receptors, Vasopressin; D014667:Vasopressins / Q000276:immunology*
chemical_list: D000888:Antibodies, Anti-Idiotypic; D007074:Immunoglobulin G; D011945:Receptors, Angiotensin; D017483:Receptors, Vasopressin; D014667:Vasopressins