[A method for determining plasmin by the rate of fibrin gel lysis].
A simple and sensitive method has been developed to assess the fibrinolytic activity of plasmin from the change in the column height of fibrin gel. Two conditions were used: 1) 37 degrees C and 16 h incubation at plasmin concentrations of 0.5-50 micrograms/ml and 2) 25 degrees C and 1-2.5 h incubation at plasmin concentrations of 50-1000 micrograms/ml. The method permits to observe the kinetics of fibrinolysis at plasmin concentrations higher that 10 micrograms/ml. The results have shown that the method is applicable for quantitation of plasminogen in human plasma. The method is precise and well reproducible.
Popova
G Iu
GIu
Eremeev
N L
NL
Aĭsina
R B
RB
Kazanskaia
N F
NF
D002846:Chromatography, Affinity; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D005337:Fibrin / Q000032:analysis; D005341:Fibrinolysin / Q000032:analysis* / Q000494:pharmacology; D005342:Fibrinolysis / Q000187:drug effects*; D005782:Gels; D006801:Humans; D008722:Methods; D010958:Plasminogen / Q000032:analysis / Q000302:isolation & purification; D013300:Streptokinase / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors
D005782:Gels; D005337:Fibrin; D010958:Plasminogen; D013300:Streptokinase; D005341:Fibrinolysin
Metod opredeleniia plazmina po skorosti lizisa fibrinovogo gelia.