Detection of 5-methylcytosine removal from DNA of Friend erythroleukemia cells following exposure to several differentiating agents.
The use of a 32P-labeling procedure has allowed us to monitor the stability of 5-methylcytosine (5-mC) in the existing fraction of DNA in differentiating Friend erythroleukemia cells (FELCs). The levels of 5-mC in existing DNA of control cells remained constant for 20 h, at nearly 3.95% of total labeled cytosine. Exposure of pre-labeled cells to 5 mM N'-methylnicotinamide (N'-MN), 4 mM hexamethylene bisacetamide (HMBA), or 270 mM dimethylsulfoxide (DMSO) over the same time-period resulted in the loss of 6.1, 4.8 and 1.8%, respectively, of the 5-mC from the pool of labeled cytosine present in the existing fraction of the DNA.
Kuykendall
J R
JR
Cancer Research Laboratory, Veterans Administration Medical Center, Memphis, TN 38104.
Cox
R
R
D044503:5-Methylcytosine; D000081:Acetamides / Q000494:pharmacology; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D003596:Cytosine / Q000031:analogs & derivatives* / Q000032:analysis; D004247:DNA / Q000032:analysis*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005622:Friend murine leukemia virus; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism* / Q000473:pathology; D009536:Niacinamide / Q000031:analogs & derivatives / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000081:Acetamides; D009536:Niacinamide; D044503:5-Methylcytosine; D003596:Cytosine; D004247:DNA; C014026:hexamethylene bisacetamide; C024058:N(1)-methylnicotinamide; D004121:Dimethyl Sulfoxide