Flow-cytometric analysis of increased IgE uptake by normal eosinophils following activation with PAF-acether and other inflammatory mediators.
Using flow-cytometric analysis and a fluorescein-conjugated anti-IgE antibody, we have investigated whether normal-density human eosinophils will bind greater amounts of native IgE following activation with platelet-activating factor (PAF), leukotriene B4 (LTB4) and histamine. Incubation with PAF resulted in a large dose- and time-dependent increase in IgE binding, optimal at 10(-7) M and 30 min, respectively. LTB4 and histamine gave lower but significant increases in IgE binding. Enhanced binding of IgE by activated normal-density eosinophils is probably a consequence of enhanced Fc epsilon RII receptor expression, suggesting a functional role for this receptor in allergic inflammation.
Walsh
G M
GM
Department of Allergy, National Children's Research Centre, Tokyo, Japan.
Nagakura
T
T
Iikura
Y
Y
D000944:Antigens, Differentiation, B-Lymphocyte / Q000378:metabolism; D004804:Eosinophils / Q000502:physiology*; D005434:Flow Cytometry; D006632:Histamine / Q000494:pharmacology*; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism*; D066298:In Vitro Techniques; D007975:Leukotriene B4 / Q000494:pharmacology*; D010972:Platelet Activating Factor / Q000494:pharmacology*; D011961:Receptors, Fc / Q000378:metabolism; D017455:Receptors, IgE; D013997:Time Factors
D000944:Antigens, Differentiation, B-Lymphocyte; D010972:Platelet Activating Factor; D011961:Receptors, Fc; D017455:Receptors, IgE; D007975:Leukotriene B4; D007073:Immunoglobulin E; D006632:Histamine