Coupled enzymatic reactions measured in a single protein crystal from myogen A.
Pairwise coupled reactions of fructose-1,6-bisphosphate aldolase and sn-glycerol-3-phosphate dehydrogenase, 3-phosphoglycerate kinase and D-glyceraldehyde-3-phosphate dehydrogenase, triosephosphate isomerase and sn-glycerol-3-phosphate dehydrogenase have been detected by microspectrophotometry in single crystals obtained from myogen A in the presence of polyethylene glycol. Microspectrophotometric measurements with polarized light demonstrate that the protein molecules are oriented and that NADH is bound with a definite orientation to the dehydrogenases within the crystal.
Keleti
T
T
Institute of Enzymology, Hungarian Academy of Sciences, Budapest.
Berni
R
R
Vas
M
M
Mozzarelli
A
A
Rossi
G L
GL
D000818:Animals; D003460:Crystallization; D004798:Enzymes / Q000378:metabolism; D006019:Glycolysis; D066298:In Vitro Techniques; D009124:Muscle Proteins / Q000378:metabolism* / Q000648:ultrastructure; D009243:NAD / Q000378:metabolism; D011817:Rabbits; D013053:Spectrophotometry
D004798:Enzymes; D009124:Muscle Proteins; D009243:NAD