Assay method for single-chain urokinase-type plasminogen activator.
A new assay method for measuring single-chain urokinase-type plasminogen activator (scu-PA) activity by quantitative calorimetric assay method is described. The serine enzyme u-PA activates plasminogen to plasmin, and scu-PA is a precursor protein obtained from a tissue culture of human kidney cells. The scu-PA solution was activated by plasmin to two-chain u-PA, which releases p-nitroaniline from pyro-Glu-Gly-Arg-pNA, and the optical density at 405 nm was measured. One unit of scu-PA was defined on the basis of 1 nmol of p-nitroaniline released from the substrate per second. One unit of scu-PA was found to be equivalent to 335 u-PA (two-chain) units (IU).
Matsuo
O
O
Department of Physiology, Kinki University School of Medicine, Osakasayama, Japan.
Ueshima
S
S
Okada
K
K
Fukao
H
H
Ohgaki
M
M
Watanabe
R
R
Arimura
H
H
D002151:Calorimetry; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D005341:Fibrinolysin / Q000494:pharmacology*; D006801:Humans; D009842:Oligopeptides / Q000378:metabolism; D010960:Plasminogen Activators / Q000032:analysis* / Q000378:metabolism; D013997:Time Factors; D014568:Urokinase-Type Plasminogen Activator / Q000032:analysis* / Q000378:metabolism
D009842:Oligopeptides; C028190:5-oxo-prolyl-glycyl-arginine-4-nitroanilide; D010960:Plasminogen Activators; D005341:Fibrinolysin; D014568:Urokinase-Type Plasminogen Activator