In vitro motor activity of intestinal segments of streptozotocin diabetic rats.
The spontaneous motor activity of the isolated segments of the gut of streptozotocin-diabetic rats was studied in vitro. In normal preparations, the frequency of intraluminal pressure changes was little influenced by raising the intraluminal pressure. In contrast, in diabetic preparations, the frequency tended to increase with a rise of the intraluminal pressure, and the pressure waves were more irregular than in controls. The motor frequency with a pressure load of 3 cm H2O in the duodenum was 33/min in control rats and 21/min in diabetic rats. The motor frequencies in the other intestinal segments were also higher in normal preparations than in diabetic ones, although these differences were statistically not significant. The amplitude of intraluminal pressure changes increased according to the increase of pressure load, both in normal and diabetic preparations. These amplitudes were higher in normal than in diabetic preparations; however, the differences were statistically significant only in the jejunum. Insulin treatment (5 U/day) for one month, one to two months after streptozotocin injection, lowered the plasma glucose level to nearly normal and increased the body weight up to 80% of the normal but did not re-establish the normal motor frequency in the duodenum. Moreover, treatment of diabetic rats with aldose reductase inhibitor, ONO-2235 (per os, 50 mg/kg b.w./day for one month, one to two months after streptozotocin injection) did not re-establish the normal rhythms in duodenum. The pacemaker activity as well as mechanical properties in the intestinal tract may be disturbed in diabetic preparations.
Karakida
T
T
Department of Physiology, Niigata University School of Medicine, Japan.
Ito
S
S
Homma
S
S
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000503:physiopathology*; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology; D007422:Intestines / Q000187:drug effects / Q000503:physiopathology*; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D012236:Rhodanine / Q000031:analogs & derivatives / Q000494:pharmacology; D013311:Streptozocin; D053778:Thiazolidines
D007328:Insulin; D053778:Thiazolidines; C038131:epalrestat; D013311:Streptozocin; D012236:Rhodanine