Magnesium ion: a possible physiological regulator of aldosterone production.
We examined the direct effect of magnesium ion on aldosterone production by adrenal cells using collagenase-dispersed zona-glomerulosa cells in rats. The effects of magnesium on aldosterone production stimulated by angiotensin II or ACTH were also investigated. Both magnesium sulphate (MgSO4) and magnesium chloride (MgCl2) (0 to 2 mM) decreased aldosterone production in a dose-dependent manner. In comparison with magnesium-free medium, 2 mM MgSO4 inhibited aldosterone production by 73% and MgCl2 by 65%. In addition, MgSO4 showed an inhibitory effect on aldosterone production stimulated by angiotensin II (10pM to 10nM), whereas it had no significant effect on aldosterone production due to ACTH stimulation (10pM to 10nM). These data suggest that magnesium has an inhibitory action on aldosterone production in vitro and may be a physiological regulator of aldosterone production.
Atarashi
K
K
Second Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Matsuoka
H
H
Takagi
M
M
Sugimoto
T
T
D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000450:Aldosterone / Q000096:biosynthesis*; D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D002413:Cations, Divalent; D005260:Female; D008274:Magnesium / Q000494:pharmacology*; D015636:Magnesium Chloride; D008278:Magnesium Sulfate / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D015384:Zona Glomerulosa / Q000187:drug effects / Q000378:metabolism*
D002413:Cations, Divalent; D015636:Magnesium Chloride; D000804:Angiotensin II; D000450:Aldosterone; D008278:Magnesium Sulfate; D000324:Adrenocorticotropic Hormone; D008274:Magnesium