Presence on a human melanoma of multiple antigens recognized by autologous CTL.
We derived from blood lymphocytes of a melanoma patient a large number of cytolytic T-cell clones directed against a cell line of the autologous tumor. Three distinct groups of antigens were recognized by these CTL on the autologous melanoma cells: group A consisted of stable antigens present on all sublines, whereas antigens B and C appeared unstable and were expressed by distinct sublines. In vitro immunoselections with various anti-A CTL clones were applied to the melanoma cells and variants resistant to 3 different CTL clones were obtained. These variants remained sensitive to other anti-A CTL clones, indicating that group A comprises at least 4 different antigens (D, E, F and A'). From a total of 76 CTL clones obtained from lymphocytes collected from the patient at various times, we found that 45 were anti-B, 17 were anti-C, 2 were anti-D, 9 were anti-E, 2 were anti-F and I was anti-A'. It is therefore likely that the 6 antigens identified by these CTL clones represent all or nearly all the transplantation antigens recognized by autologous CTL on this human melanoma.
Van den Eynde
B
B
Ludwig Institute for Cancer Research, UCL 7459, Brussels, Belgium.
Hainaut
P
P
Hérin
M
M
Knuth
A
A
Lemoine
C
C
Weynants
P
P
van der Bruggen
P
P
Fauchet
R
R
Boon
T
T
D000951:Antigens, Neoplasm / Q000032:analysis* / Q000145:classification / Q000235:genetics; D002460:Cell Line; D002999:Clone Cells / Q000276:immunology; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D005805:Genes, MHC Class I / Q000276:immunology; D014644:Genetic Variation / Q000235:genetics / Q000276:immunology; D006680:HLA Antigens / Q000145:classification; D006801:Humans; D007959:Lymphocyte Culture Test, Mixed; D008545:Melanoma / Q000235:genetics / Q000276:immunology*; D012878:Skin Neoplasms / Q000235:genetics / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D006680:HLA Antigens