title: Stimulatory effect of avian influenza virus on chicken lymphocytes.
abstract: A study was conducted to examine the effect of avian influenza virus (AIV) on chicken lymphocyte activation. Unprimed or Brucella abortus antigen (Ag)-primed lymphocytes were incubated with various doses of the T-cell mitogen concanavalin A (Con A) or Ag, respectively, plus serial dilutions of inactivated AIV for 72 hr, and cell proliferation was measured via uptake of tritiated thymidine. AIV enhanced the proliferative response to Con A or Ag by 150% or better, and the enhancement decreased in a viral dose-dependent manner. The effects were more readily observed in cells that had not been maximally activated by the Con A or Ag. The enhanced response was observed in lymphocytes from both white rock and white leghorn breeds of chicken and in mature peripheral blood lymphocytes or immature thymocytes. The viral activity could be abrogated by pre-treatment of the viral preparation with AIV-specific antisera or prior adsorption of the AIV with chicken erythrocytes. These results indicate that AIV can interact with and modify the in vitro activity of chicken lymphocytes and may exert modulatory effects on the avian immune system.
authors.affiliation: U.S. Department of Agriculture, Southeast Poultry Research Laboratory, Athens, Georgia 30605.
authors.forename: P S
authors.initials: PS
authors.lastname: Holt
authors.forename: J W
authors.initials: JW
authors.lastname: Latimer
mesh_terms: D000818:Animals; D002645:Chickens / Q000276:immunology* / Q000382:microbiology; D003208:Concanavalin A; D004912:Erythrocytes / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D009975:Orthomyxoviridae / Q000472:pathogenicity*; D013047:Specific Pathogen-Free Organisms
chemical_list: D003208:Concanavalin A