A new Zn2+-stimulated sphingomyelinase in fetal bovine serum.
Fetal bovine serum contains a Zn2+-dependent sphingomyelinase with optimal activity at pH 5.5 in vitro. Activity could be demonstrated with a liposomal sphingomyelin substrate suspension but was stimulated up to 15-fold by Triton X-100. Under a variety of conditions tested, phosphatidylcholine, lysophosphatidylcholine, glycerophosphocholine, and p-nitrophenyl phosphate were not substrates for this activity. Several inhibitors of serum alkaline and acid phosphatases had no effect on the activity. The enzyme resembles the acid lysosomal sphingomyelinase in pH optimum and inhibition by AMP but differs in inhibition by EDTA, stimulation by Zn2+, and heat lability at 55 degrees C. It resembles the neutral, Mg2+-stimulated enzyme in inhibition by EDTA and heat lability but differs in metal ion requirement and pH optima. Of the sera tested, activity was highest in fetal bovine serum, with fetal bovine greater than newborn bovine greater than horse greater than human; more than 95% of the activity is in the lipoprotein-free infranatant of serum (d greater than 1.21). This activity appears to be a hitherto undescribed sphingomyelinase. Its biological functions are not known but may subserve a special role in sphingomyelin catabolism in the circulation, in blood vessel walls, or in the tissue(s) of origin.
Spence
M W
MW
Department of Pediatrics, Dalhousie University, Halifax, Nova Scotia, Canada.
Byers
D M
DM
Palmer
F B
FB
Cook
H W
HW
D000818:Animals; D002413:Cations, Divalent; D002417:Cattle; D002712:Chlorides / Q000494:pharmacology*; D003902:Detergents / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D005312:Fetal Blood / Q000201:enzymology; D006736:Horses; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D007700:Kinetics; D017830:Octoxynol; D010727:Phosphoric Diester Hydrolases / Q000097:blood*; D011092:Polyethylene Glycols / Q000494:pharmacology; D013045:Species Specificity; D013108:Sphingomyelin Phosphodiesterase / Q000097:blood*; D015032:Zinc / Q000494:pharmacology*; D017967:Zinc Compounds
D002413:Cations, Divalent; D002712:Chlorides; D003902:Detergents; D017967:Zinc Compounds; D011092:Polyethylene Glycols; C016837:zinc chloride; D017830:Octoxynol; D004492:Edetic Acid; D010727:Phosphoric Diester Hydrolases; D013108:Sphingomyelin Phosphodiesterase; D015032:Zinc