title: Simple and rapid purification of F1-ATPase from mitochondria.
abstract: A simple, rapid method for isolation of F1-ATPase from bovine heart mitochondria using the cation exchange resin Dynospheres PD-201-SCX was developed. The method is based on the fact that hydrophobic membrane proteins are adsorbed efficiently on this resin, whereas hydrophilic proteins, such as F1-ATPase, are not. By this method F1-ATPase of high purity and enzyme activity can be obtained from mitochondria in about 2 h.
authors.affiliation: Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Shinohara
authors.forename: M
authors.initials: M
authors.lastname: Nakagawa
authors.forename: H
authors.initials: H
authors.lastname: Terada
mesh_terms: D000818:Animals; D002417:Cattle; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D008565:Membrane Proteins / Q000302:isolation & purification; D008929:Mitochondria, Heart / Q000201:enzymology*; D010085:Oxidative Phosphorylation; D006180:Proton-Translocating ATPases / Q000302:isolation & purification* / Q000378:metabolism; D012116:Resins, Plant; D013997:Time Factors
chemical_list: D008565:Membrane Proteins; D012116:Resins, Plant; D006180:Proton-Translocating ATPases