Ajoene, the antiplatelet compound derived from garlic, specifically inhibits platelet release reaction by affecting the plasma membrane internal microviscosity.
Ajoene (E,Z-4,5,9-trithiadodeca-1,6,11-triene 9-oxide), a product of the rearrangement of allicin (a major component of raw garlic), has been shown to be a potent inhibitor of platelet aggregation in vitro through inhibition of granule release and fibrinogen binding. Our present study further elaborates on this inhibitory action, through studies of the effect of ajoene on the earliest steps of platelet activation. The transducing mechanism involved in thrombin-induced platelet activation was not modified by the drug as indicated by a normal breakdown of phosphatidylinositol 4,5,bisphosphate and normal production of phosphatidic acid. Likewise, the agonist-induced phosphorylation of myosin light chain (P20) and of the 43 kD protein (P43) were not impaired by ajoene. Under the same conditions, however, ajoene (100 microM) produced a strong inhibition of the thrombin-induced release of dense body and alpha-granule constituents. Electron spin resonance studies of the effect of ajoene on some physico-chemical properties of the platelet plasma membrane (intact platelets), as well as on artificial lipid membranes, indicated that ajoene increased mobility of the fatty acid spin label 16 nitroxide stearate. This suggests the existence of a decreased microviscosity of the most internal region within the lipid bilayer membrane, without affecting the outer hydrophilic moieties of the bilayer. As a whole, these results suggest that the effect of ajoene on the release reaction must be, in part, due to physical modification of the bilayer, which impairs the fusion of the granules and plasma membrane, a prerequisite for exocytosis.
Rendu
F
F
U 150 INSERM, URA 184 CNRS, Hôpital Lariboisière, Paris, France.
Daveloose
D
D
Debouzy
J C
JC
Bourdeau
N
N
Levy-Toledano
S
S
Jain
M K
MK
Apitz-Castro
R
R
D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D004220:Disulfides / Q000494:pharmacology*; D004578:Electron Spin Resonance Spectroscopy; D066298:In Vitro Techniques; D008560:Membrane Fluidity / Q000187:drug effects*; D008567:Membranes, Artificial; D010716:Phosphatidylinositols / Q000097:blood; D010750:Phosphoproteins / Q000096:biosynthesis; D010936:Plant Extracts / Q000494:pharmacology*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D012701:Serotonin / Q000097:blood; D013454:Sulfoxides; D013931:Thromboxanes / Q000096:biosynthesis; D001620:beta-Thromboglobulin / Q000378:metabolism
D004220:Disulfides; D008567:Membranes, Artificial; D010716:Phosphatidylinositols; D010750:Phosphoproteins; D010936:Plant Extracts; D010975:Platelet Aggregation Inhibitors; D013454:Sulfoxides; D013931:Thromboxanes; D001620:beta-Thromboglobulin; D012701:Serotonin; C048980:ajoene