Selectable recombinant herpesvirus saimiri is capable of persisting in a human T-cell line.
Strategies were developed to insert the neo resistance marker into the junction between L DNA and the right terminal repetitive H DNA of herpesvirus saimiri. Recombinant viruses were selectable in permissive epithelioid cultures. The human T-cell line Jurkat could be infected persistently with the Neor virus; the cells contained episomal viral DNA in high copy number. This selectable vector should be generally applicable for gene expression in human T cells.
Grassmann
R
R
Institut für Klinische und Molekulare Virologie, Universität Erlangen-Nürnberg, Federal Republic of Germany.
Fleckenstein
B
B
D002460:Cell Line; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D005822:Genetic Vectors; D006567:Herpesvirus 2, Saimiriine / Q000235:genetics / Q000254:growth & development*; D006801:Humans; D011995:Recombination, Genetic; D013601:T-Lymphocytes / Q000382:microbiology*
D004274:DNA, Recombinant