Flow cytometric enumeration of colonies for in vitro chemotherapeutic drug evaluation.
The percentage of 50-100 micron colonies formed by LX-T cells in medium containing agarose was determined microscopically, and this value was compared with the percentage determined by a flow cytometric method based on the forward and 90 degree light scatter of the colonies. As assessed by both in vitro methods, LX-T cells exposed to chemotherapeutic agents formed fewer colonies as the drug concentration increased. However, flow cytometric analysis indicated that a change in the number of colonies formed was a consequence of changing chemotherapeutic drug concentration, whereas microscopic colony counting did not always detect the corresponding change in colony number. These experiments demonstrate that measurement of a drug's chemotherapeutic potential by flow cytometric counting of colonies is an alternative to the enumeration of colonies microscopically.
Thornthwaite
J T
JT
Pathology Department, Baptist Hospital, Miami, Florida 33176.
Loutzenhiser
K
K
Hornicek
F J
FJ
Donahue
R
R
Allen
L M
LM
D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use; D018288:Carcinoma, Small Cell / Q000188:drug therapy; D002460:Cell Line; D004317:Doxorubicin / Q000494:pharmacology / Q000627:therapeutic use; D004341:Drug Evaluation / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D008297:Male; D051379:Mice; D008875:Middle Aged; D008937:Mitomycins / Q000494:pharmacology / Q000627:therapeutic use; D014411:Neoplastic Stem Cells / Q000166:cytology* / Q000187:drug effects
D000970:Antineoplastic Agents; D008937:Mitomycins; D004317:Doxorubicin