Immunoprotective extracellular polysaccharides of Klebsiella aerogenes capsular type K1, expressed in Escherichia coli.
A gene library of genomic DNA Klebsiella aerogenes of capsular serotype K1 was constructed in E. coli LE392 using the cosmid pMMB33. Culture filtrates of E. coli recombinants were screened by ELISA for extracellular polysaccharides specific for K. aerogenes K1. Extracellular polysaccharide extracts from K. aerogenes K1 and 3% of the E. coli recombinants contained immunoprotective extracellular polysaccharides (IEP) with similar chemical and immunological properties as shown by gel filtration through Sephacryl 1000, double immunodiffusion and mouse protection tests. IEPs contained no detectable protein, had molecular weights of several hundred million and protected mice against lethal autologous K. aerogenes K1 challenge at a dosage of 10 nanograms per mouse.
Roe
E A
EA
MRC Pathogenicity Group, University of Liverpool, U.K..
Jones
R J
RJ
Saunders
J R
JR
D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000302:isolation & purification; D016667:Bacterial Capsules; D003360:Cosmids; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology; D015723:Gene Library; D005822:Genetic Vectors; D007114:Immunization; D005779:Immunodiffusion; D007710:Klebsiella Infections / Q000517:prevention & control; D007711:Klebsiella pneumoniae / Q000235:genetics / Q000276:immunology*; D051379:Mice; D011135:Polysaccharides, Bacterial / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification
D000942:Antigens, Bacterial; D011135:Polysaccharides, Bacterial; C036962:capsular polysaccharide K1