title: Strychnine-enhanced transsynaptic degeneration of dorsal horn neurons in rats with an experimental painful peripheral neuropathy.
abstract: A painful peripheral neuropathy was produced by loosely tying constrictive ligatures around the sciatic nerve of rats. Eight days after the nerve injury and after a series of injections a subconvulsive dose of strychnine, the animals were sacrificed and the spinal cord dorsal horn was examined for signs of transsynaptic degeneration. The presence of strychnine-enhanced transsynaptic degeneration was apparent from the presence of many pyknotic and hyperchromatic neurons (dark neurons). The dark neurons were seen in laminae I-IV bilaterally, but they were significantly more frequent ipsilateral to the nerve injury. The majority of the dark neurons were found within the medial two-thirds of laminae I-II; this region corresponds to the intraspinal territory of the sciatic nerve's small diameter afferents. The results suggest that a peripheral nerve injury that produces neuropathic pain induces morphological alterations of intraspinal somatosensory circuitry.
authors.affiliation: Second Department of Oral Anatomy, Faculty of Dentistry, Osaka University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sugimoto
authors.forename: G J
authors.initials: GJ
authors.lastname: Bennett
authors.forename: K C
authors.initials: KC
authors.lastname: Kajander
mesh_terms: D000818:Animals; D008297:Male; D009410:Nerve Degeneration / Q000187:drug effects*; D010146:Pain / Q000209:etiology / Q000503:physiopathology; D010523:Peripheral Nervous System Diseases / Q000150:complications / Q000473:pathology* / Q000503:physiopathology; D051381:Rats; D011919:Rats, Inbred Strains; D013331:Strychnine / Q000494:pharmacology*
chemical_list: D013331:Strychnine