title: Temperature-sensitive mutants of bovine herpesvirus type 1: mutants which make unaltered levels of 'early' glycoproteins but fail to synthesize a 'late' glycoprotein.
abstract: The major glycoproteins of bovine herpesvirus type 1 showed distinct temporal patterns of expression. The glycoproteins GVP 11 and GVP 6 as well as its cleavage products, GVP 11a and GVP 16, were expressed early in the infectious process, whereas GVP 9 was expressed late. Temperature-sensitive mutants were developed and characterized. Mutants belonging to two complementation groups were unable to synthesize DNA at 40 degrees C, the non-permissive temperature. In cells infected with these mutants the late glycoprotein GVP 9 was not synthesized at 40 degrees C, whereas the synthesis of the early glycoproteins GVP 11 and GVP 6 continued at wild-type levels. These studies suggest that the transition from early to late glycoprotein synthesis is linked to viral DNA synthesis.
authors.affiliation: Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada.
authors.forename: R
authors.initials: R
authors.lastname: Nelson
authors.forename: A M
authors.initials: AM
authors.lastname: Adachi
authors.forename: H
authors.initials: H
authors.lastname: Chisholm
authors.forename: V
authors.initials: V
authors.lastname: Misra
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D007242:Herpesvirus 1, Bovine / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D011233:Precipitin Tests; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D004279:DNA, Viral; D006023:Glycoproteins; D014764:Viral Proteins