title: T lymphocyte activation by staphylococcal enterotoxins: role of class II molecules and T cell surface structures.
abstract: The enterotoxins produced by Staphylococcus aureus (SE) are the most potent mitogens known. Triggering of proliferation or cytotoxicity by SE requires the presence of MHC class II molecules on accessory or target cells. In this study we have investigated the role of HLA class II molecules in the activation of human T cells by SE and the nature of the target structure on the responding T lymphocyte for SE. This dependence on class II molecules is not due to an immunological "recognition" of SE since there is no restriction by polymorphic determinants of HLA molecules and since even xenogeneic class II molecules can reconstitute the human T cell response to SE. Furthermore, HLA class II-positive but not -negative cells absorb the mitogenic activity from SE solutions and significant binding of 125I-labeled SE can be demonstrated to class II-positive but not to class II-negative cells. Enterotoxin molecules react directly with T cells since they cause an increase in cytosolic Ca2+ concentration similar to anti-CD3 mAb. This increase is abrogated by prior modulation of the TCR/CD3 complex. Antibodies to CD2, CD3 and the TCR that block antigen-specific activation also block T cell activation by SE. Moreover, preincubation of purified resting accessory cell-free T cells with SE leads to modulation of the TCR/CD3 complex. Taken together these data indicate that SE interact selectively with HLA class II molecules on accessory or target cells and with a TCR-associated structure on the T cell.
authors.affiliation: Department of Medical Microbiology and Immunology, University of Ulm, Federal Republic of Germany.
authors.forename: B
authors.initials: B
authors.lastname: Fleischer
authors.forename: H
authors.initials: H
authors.lastname: Schrezenmeier
authors.forename: P
authors.initials: P
authors.lastname: Conradt
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D017252:CD3 Complex; D002118:Calcium / Q000502:physiology; D002478:Cells, Cultured; D004768:Enterotoxins / Q000494:pharmacology*; D005434:Flow Cytometry; D006681:HLA-D Antigens / Q000235:genetics / Q000276:immunology*; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D051379:Mice; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D014162:Transfection
chemical_list: D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D004768:Enterotoxins; D006681:HLA-D Antigens; D011948:Receptors, Antigen, T-Cell; C026066:enterotoxin A, Staphylococcal; C031818:enterotoxin B, staphylococcal; D002118:Calcium