IL-1 as adjuvant. Role of T cells in the augmentation of specific antibody production by recombinant human IL-1 alpha.
Human rIL-1 alpha significantly enhanced splenic plaque-forming cells (PFC) to SRBC in vitro and in vivo. A single i.p. injection was sufficient to produce a fivefold or greater increase in the generation of PFC in a primary response. IL-1 treatment resulted in an increased production of Ag-specific PFC, both in vitro and in vivo, in combination with suboptimal doses of Ag. When IL-1 was given with a primary dose of Ag in vivo, an enhanced IgG response occurred. IL-1 enhanced in vivo carrier priming for an anti-hapten PFC response, indicating increased Th activity. Furthermore, T cells from spleens of mice treated with IL-1 provided significantly more help in both carrier (SRBC)- and hapten (TNP)- specific PFC. The enhancement of PFC by IL-1 in vitro occurred even in the presence of an excess of neutralizing anti-IL-2 antibody. These results suggest that IL-1 may enhance T cell-dependent antibody production in part by increasing Th activity, and that the mechanism of IL-1 action in increasing antibody production involves pathways in addition to the induction of IL-2 secretion.
Reed
S G
SG
Seattle Biomedical Research Institute, WA 98109.
Pihl
D L
DL
Conlon
P J
PJ
Grabstein
K H
KH
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D001667:Binding, Competitive; D005260:Female; D006462:Hemolytic Plaque Technique; D007106:Immune Sera / Q000494:pharmacology; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D007375:Interleukin-1 / Q000276:immunology / Q000494:pharmacology*; D015847:Interleukin-4; D007378:Interleukins / Q000276:immunology; D051379:Mice; D011994:Recombinant Proteins / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000502:physiology*; D006377:T-Lymphocytes, Helper-Inducer / Q000187:drug effects
D000276:Adjuvants, Immunologic; D007106:Immune Sera; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007375:Interleukin-1; D007378:Interleukins; D011994:Recombinant Proteins; D015847:Interleukin-4