Rapid determination of the benzodiazepine antagonist flumazenil, Ro 15-1788, by high performance liquid chromatography.
A sensitive (2 ng/mL) and specific method for the determination of the benzodiazepine antagonist Ro 15-1788 or flumazenil is described. Following a simple extraction, the compound is analyzed by reversed phase high performance liquid chromatography (HPLC) and detection at 245 nm. The method was applied to plasma specimens collected from patients receiving a single dose of this drug.
Bun
H
H
Laboratoire de Pharmacocinétique et Toxicocinétique, CHU TIMONE, Marseille, France.
Duplan
V
V
Crevat-Pisano
P
P
Llurens
M
M
Durand
A
A
D001569:Benzodiazepines / Q000037:antagonists & inhibitors*; D002851:Chromatography, High Pressure Liquid; D005442:Flumazenil / Q000032:analysis*; D006801:Humans; D011786:Quality Control
D001569:Benzodiazepines; D005442:Flumazenil