Regulation of Fc epsilon receptor 2 (CD23) expression on a human eosinophilic cell line EoL3 and a human monocytic cell line U937 by transforming growth factor beta.
Regulation of low-affinity receptors for IgE (Fc epsilon R2/CD23) on a human eosinophilic cell line EoL3 and a human monocytic cell line U937 was studied using an anti-Fc,R2/CD23 monoclonal antibody H107 by flow cytometry. While platelet-activating factor, interleukin 4, and interferon gamma significantly augmented Fc epsilon R2/CD23 expression on both cell lines, transforming growth factor beta (TGF beta) inhibited both the basal level of Fc epsilon R2/CD23 expression and the enhanced Fc epsilon R2/CD23 expression induced by these reagents in dose- and time-dependent manners. However, TGF beta did not significantly suppress the high basal level of Fc epsilon R2/CD23 expression on RPMI 8866 cells. These results suggest that Fc epsilon R2/CD23 expression on EoL3 and U937 cells is regulated by various cytokines and growth factors, and that TGF beta plays an important regulatory role in IgE-mediated immune responses.
Tanaka
M
M
Department of Pediatrics, Faculty of Medicine, Kyoto University, Japan.
Lee
K C
KC
Yodoi
J
J
Saito
H
H
Iwai
Y
Y
Kim
K M
KM
Morita
M
M
Mayumi
M
M
Mikawa
H
H
D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis*; D002461:Cell Line, Transformed; D003907:Dexamethasone / Q000494:pharmacology; D004804:Eosinophils / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D015847:Interleukin-4; D007378:Interleukins / Q000494:pharmacology; D015472:Leukemia, Eosinophilic, Acute / Q000276:immunology; D007951:Leukemia, Myeloid / Q000276:immunology; D009000:Monocytes / Q000276:immunology*; D010972:Platelet Activating Factor / Q000494:pharmacology; D011961:Receptors, Fc / Q000032:analysis*; D017455:Receptors, IgE; D015291:Transforming Growth Factors / Q000494:pharmacology*
D000944:Antigens, Differentiation, B-Lymphocyte; D007378:Interleukins; D010972:Platelet Activating Factor; D011961:Receptors, Fc; D017455:Receptors, IgE; D015847:Interleukin-4; D015291:Transforming Growth Factors; D003907:Dexamethasone; D007371:Interferon-gamma