[Cryotransformation of Bacillus anthracis by the DNA of pUB110 plasmid].
Possibility of cryotransformation of Bacillus anthracis cells by the DNA of pUB110 plasmid has been established. The parameters of cryotransformation process have been optimized permitting one to increase the efficiency of transformation up to 3.1 . 10(2) transformants per 1 mkg of transforming DNA. The factors affecting the efficiency of cryotransformation and its reproducibility have been studied including the treatment of recipient cells by glycine, the procedure of freeze-thawing, the composition of freezing medium. The recipient activity of Bacillus anthracis cells has been shown to depend on the set of their own plasmids.
Puzanova
O B
OB
Stepanov
A S
AS
Beliakova
N I
NI
Loginova
O G
OG
Il'iashenko
B N
BN
Ditatkin
S Ia
SIa
D001408:Bacillus anthracis / Q000235:genetics* / Q000254:growth & development; D001412:Bacillus subtilis / Q000235:genetics; D003470:Culture Media; D004269:DNA, Bacterial / Q000235:genetics*; D005615:Freezing; D010957:Plasmids; D014169:Transformation, Bacterial
D003470:Culture Media; D004269:DNA, Bacterial
Kriotransformatsiia sibireiazvennogo mikroba DNK plazmidy pUB110.