Sequence and expression of a gene from Theileria annulata coding for a 70-kilodalton heat-shock protein.
A library consisting of randomly sheared Theileria annulata genomic DNA fragments in the vector lambda gt11 was screened with a Drosophila DNA segment containing the coding region of the 70-kDa heat-shock protein (hsp) gene. A positive recombinant was isolated and subjected to nucleotide sequence analysis. The DNA segment contains an open reading frame coding for a 71-kDa protein strongly homologous to hsp 70 from other organisms. Using this DNA as a probe, a homologous 2.5-kb RNA species was detected in sporozoites, piroplasms and a macroschizont-infected cell line, showing that this gene is constitutively expressed. The amount of this RNA increased following heat shock in the macroschizont-infected cell line. The T. annulata genome contains other sequences that hybridize weakly with this heat-shock gene.
Mason
P J
PJ
Department of Haematology, Royal Postgraduate Medical School, London, U.K.
Shiels
B R
BR
Tait
A
A
Beck
P
P
Hall
R
R
D000595:Amino Acid Sequence; D000818:Animals; D016782:Apicomplexa / Q000235:genetics*; D001483:Base Sequence; D002417:Cattle; D002460:Cell Line; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D005796:Genes; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D004247:DNA