[Air-liquid interface cultivation of human keratinocytes].
For simulation of in vivo conditions, air-liquid interface cultivation of human keratinocytes is reported. After human keratinocytes were grown on a collagen matrix inside plastic rings at the air-liquid interface for 14 days, the culture was cryostat sectioned and labeled with monoclonal antibody against human filaggrin using the APAAP technique. The culture displayed a stratified, multilayered epithelium with a basal cell layer and 9-11 supra-basal layers. Filaggrin was found mainly in the upper cell layers. As the microarchitecture of the culture was essentially similar to in vivo conditions, air-liquid interface cultivation seems to be an appropriate method for the study of epidermal cell growth and differentiation in vitro.
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D000388:Air; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D000091344:Filaggrin Proteins; D006801:Humans; D015603:Keratinocytes / Q000166:cytology*