Supercoil-stabilized left-handed DNA in the plasmid (dA-dT)16 insert formed in the presence of Ni2+.
The (dA-dT)16 insert of the plasmid pAT32 was probed with diethyl pyrocarbonate (DEPC) and nuclease Bal3l in the presence of Ni2+ known to be able to induce transition to left-handed conformation in the synthetic poly(dA-dT).poly(dA-T). It has been shown that this insert in a supercoiled plasmid displays a DEPC modification pattern characteristic of left-handed DNA under conditions not sufficient to induce a left-handed structure in the linear plasmid and poly(dA-dT).poly(dA-T).
Nejedlý
K
K
Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Lódź.
Kłysik
J
J
Palecek
E
E
D004251:DNA Transposable Elements / Q000187:drug effects*; D004278:DNA, Superhelical / Q000032:analysis / Q000187:drug effects*; D004047:Diethyl Pyrocarbonate; D004706:Endodeoxyribonucleases; D009532:Nickel / Q000494:pharmacology*; D009690:Nucleic Acid Conformation; D009710:Nucleotide Mapping; D010957:Plasmids; D011067:Poly dA-dT / Q000032:analysis*; D011089:Polydeoxyribonucleotides / Q000032:analysis*
D004251:DNA Transposable Elements; D004278:DNA, Superhelical; D011089:Polydeoxyribonucleotides; D011067:Poly dA-dT; D009532:Nickel; D004706:Endodeoxyribonucleases; C038407:exonuclease Bal 31; D004047:Diethyl Pyrocarbonate