Atrial natriuretic factor in the Langendorff perfused coronary vasculature of the rabbit isolated heart.
Removal of exogenously administered rat ANF (99-126) (rANF) from the rabbit coronary vasculature was investigated. Rabbit hearts were perfused using a modified Langendorff technique and ANF concentrations in the perfusate were measured by a radio-receptor assay. Under these conditions no major degradation of ANF was observed. On perfusion, however, the heart liberated large amounts of ANF. This release peaked 15 minutes after the initiation of perfusion, (685 + 220 pM) and then fell to a sustained basal level (305 + 80 pM) after 45 minutes. Although an increase in the perfusate flow rate reduced the ANF concentration, there was no significant difference in the rate of ANF release between the two flow rates used. After momentary cessation of flow ANF concentration fell to a significantly lower level, however, once again no significant change in rate of release occurred. These results suggest that the heart is not a major site of ANF degradation and that alterations in flow rate through the coronary vascular bed can cause changes in amounts of ANF released.
Klair
S S
SS
Biochemistry Department, Glaxo Group Research Limited, Greenford, Middlesex, England.
Louttit
J B
JB
Charlton
P A
PA
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism* / Q000494:pharmacology; D003326:Coronary Circulation; D003331:Coronary Vessels / Q000187:drug effects / Q000502:physiology*; D004232:Diuretics / Q000494:pharmacology; D006321:Heart / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000502:physiology; D010446:Peptide Fragments / Q000494:pharmacology; D010477:Perfusion; D011817:Rabbits; D011994:Recombinant Proteins / Q000494:pharmacology
D004232:Diuretics; D010446:Peptide Fragments; D011994:Recombinant Proteins; D009320:Atrial Natriuretic Factor