Vanadate counteracts glucagon effects in isolated rat hepatocytes.
The incubation of isolated rat hepatocytes with vanadate increased the concentration of fructose 2,6-bisphosphate without modifying 6-phosphofructo-2-kinase activity. Vanadate also reverted and prevented the decrease of fructose 2,6-bisphosphate levels, of the "active" form of the 6-phosphofructo 2-kinase and of the pyruvate kinase activity ratio produced by glucagon, by probably counteracting the increase in cyclic AMP concentration.
Miralpeix
M
M
Departament de Ciències Fisiològiques Humanes i de la Nutrició, Universitat de Barcelona, Zona Universitària, Spain.
Gil
J
J
Rosa
J L
JL
Carreras
J
J
Bartrons
R
R
D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D005215:Fasting; D005502:Food; D005635:Fructosediphosphates / Q000378:metabolism; D005934:Glucagon / Q000494:pharmacology*; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010732:Phosphofructokinase-1 / Q000378:metabolism; D010766:Phosphorylation; D011770:Pyruvate Kinase / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014638:Vanadates / Q000494:pharmacology*
D005635:Fructosediphosphates; D014638:Vanadates; C027652:fructose 2,6-diphosphate; D005934:Glucagon; D000242:Cyclic AMP; D010732:Phosphofructokinase-1; D011770:Pyruvate Kinase