Membrane anchoring of a human IgG Fc receptor (CD16) determined by a single amino acid.
CD16 is a low-affinity immunoglobulin G (IgG) Fc receptor that is expressed on natural killer (NK) cells, granulocytes, activated macrophages, and some T lymphocytes. Two similar genes, CD16-I and CD16-II, encode membrane glycoproteins that are anchored by phosphatidylinositol (PI)-glycan and transmembrane polypeptides, respectively. The primary structural requirements for PI-linkage were examined by constructing a series of hybrid cDNA molecules. Although both cDNA's have an identical COOH-terminal hydrophobic segment, CD16-I has Ser203 whereas CD16-II has Phe203. Conversion of Phe to Ser in CD16-II permits expression of a PI-glycan-anchored glycoprotein, whereas conversion of Ser to Phe in CD16-I prevents PI-glycan linkage.
Lanier
L L
LL
Becton Dickinson Monoclonal Center, Inc., Mountain View, CA 94043.
Cwirla
S
S
Yu
G
G
Testi
R
R
Phillips
J H
JH
D000818:Animals; D015703:Antigens, CD / Q000235:genetics*; D000943:Antigens, Differentiation / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D003062:Codon / Q000235:genetics; D005803:Genes, Immunoglobulin; D006098:Granulocytes / Q000276:immunology; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D010649:Phenylalanine; D011961:Receptors, Fc / Q000235:genetics* / Q000378:metabolism; D017452:Receptors, IgG; D012694:Serine; D014162:Transfection
D015703:Antigens, CD; D000943:Antigens, Differentiation; D003062:Codon; D008562:Membrane Glycoproteins; D011961:Receptors, Fc; D017452:Receptors, IgG; D012694:Serine; D010649:Phenylalanine