Filamentous hemagglutinin of Bordetella pertussis: nucleotide sequence and crucial role in adherence.
Filamentous hemagglutinin is a surface-associated adherence protein of Bordetella pertussis, which is a component of some new acellular pertussis vaccines. The nucleotide sequence of an open reading frame that encompasses the filamentous hemagglutinin structural gene, fhaB, suggests that proteolytic processing is necessary to generate the mature 220-kDa filamentous hemagglutinin product. An Arg-Gly-Asp (RGD) tripeptide is found within filamentous hemagglutinin that may be involved in its adherence properties. An internal in-frame deletion in fhaB, encompassing the RGD region, causes loss of B. pertussis-binding to ciliated eukaryotic cells, confirming a potential role for this protein in host-cell binding and infection.
Relman
D A
DA
Department of Microbiology and Immunology, Stanford University 94305-5402.
Domenighini
M
M
Tuomanen
E
E
Rappuoli
R
R
Falkow
S
S
D000595:Amino Acid Sequence; D000818:Animals; D000954:Antigens, Surface / Q000235:genetics; D001422:Bacterial Adhesion; D001483:Base Sequence; D001886:Bordetella pertussis / Q000235:genetics*; D015815:Cell Adhesion Molecules; D002460:Cell Line; D003001:Cloning, Molecular; D006224:Cricetinae; D005798:Genes, Bacterial; D006388:Hemagglutinins / Q000235:genetics*; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D012045:Regulatory Sequences, Nucleic Acid; D012137:Respiratory System / Q000382:microbiology
D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D006388:Hemagglutinins