Molecular cloning of the complete genome of strain GDVII of Theiler's virus and production of infectious transcripts.
We constructed a complete cDNA clone of strain GDVII of Theiler's virus in Bluescript plasmid. This recombinant plasmid, called pTMGDVII, was used to synthesize full-length RNA transcripts of the viral insert. This RNA was infectious for BHK cells. Virus R1-GDVII, obtained from transfected BHK cells, caused rapidly fatal encephalomyelitis in BALB/c mice. High amounts of viral antigens were present in neurons. No antigens were found in white matter. Therefore, the phenotype of R1-GDVII was indistinguishable from that of genuine GDVII Theiler's virus.
Tangy
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F
Unité Virus Lents, UA 1157 Centre National de la Recherche Scientifique, Institut Pasteur, Paris, France.
McAllister
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A
Brahic
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M
D000818:Animals; D000956:Antigens, Viral / Q000096:biosynthesis / Q000235:genetics; D002213:Capsid / Q000235:genetics / Q000276:immunology; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004770:Enterovirus / Q000235:genetics*; D004680:Maus Elberfeld virus / Q000235:genetics* / Q000276:immunology; D051379:Mice; D012367:RNA, Viral / Q000235:genetics; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014162:Transfection; D014779:Virus Replication
D000956:Antigens, Viral; D012367:RNA, Viral; D004247:DNA