The temperature dependence of the time course of growth and decay of miniature end-plate currents in carp extraocular muscle following thermal acclimation.
1. The effect of temperature (5-35 degrees C) on the decay and growth phases of miniature end-plate currents (MEPCs) was investigated in extraocular muscle from freshwater carp acclimated to either high (28 degrees C) or low (8 degrees C) temperature. 2. The temperature dependence of the time constant of decay (TD) was found to follow an Arrhenius relationship; the relationship between logTD and reciprocal of absolute temperature (1/K) being linear in both groups. The TD of MEPCs recorded from cold-acclimated carp was not statistically significant from that of the warm group. 3. TD was moderately temperature-dependent. Regression gave a Q10 of 1.78 for the warm-acclimated carp, corresponding to an activation energy, Ea, of 41.15 +/- 2.17 kJ mol-1. For the cold-acclimated carp, the Q10 was 1.79, and Ea was 41.43 +/- 2.46 kJ mol-1. 4. Growth time (TG) was less susceptible than TD to temperature change. The relationship between growth time (taken as the time for MEPCs to rise from 20 to 80% of maximum) and temperature was linear for the cold-acclimated group, with a Q10 of 1.34 and Ea of 20.94 +/- 4.75 kJ mol-1. The data for the warm group, were, in contrast, best fitted by two linear regressions meeting at 15.1 degrees C. At temperatures below 15.1 degrees C Q10 was 3.16 and Ea was 82.20 +/- 15.47 kJ mol-1; above 15 degrees C, Q10 was 1.22 and Ea was 14.15 +/- 12.24 kJ mol-1. 5. The acetylcholinesterase inhibitor neostigmine increased TD by approximately twofold and raised TG to approximately 1.4 times control values. These effects were observed across the temperature range scanned for both groups. 6. The results are discussed with reference to the documented effects of temperature and temperature acclimation on membrane lipids and proteins.
Harper
A A
AA
Department of Anatomy and Physiology, Old Medical School, The University, Dundee, UK.
Shelton
J R
JR
Watt
P W
PW
D000064:Acclimatization; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D002347:Carps / Q000502:physiology*; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D003530:Cyprinidae / Q000502:physiology*; D004563:Electrochemistry; D009210:Myofibrils / Q000187:drug effects / Q000201:enzymology / Q000502:physiology; D009469:Neuromuscular Junction; D009801:Oculomotor Muscles / Q000502:physiology*; D013696:Temperature; D013997:Time Factors
D002800:Cholinesterase Inhibitors; D000251:Adenosine Triphosphatases