Carbohydrates on human Fc gamma receptors. Interdependence of the classical IgG and nonclassical lectin-binding sites on human Fc gamma RIII expressed on neutrophils.
To explore the molecular basis for the ability of aggregated IgG to block the phagocytosis by human polymorphonuclear leukocytes of Con A-opsonized E and of nonopsonized Escherichia coli with mannose-binding adhesins, we examined specific aspects of the glycoprotein structure of both the 40- to 43-kDa receptor for the Fc portion of IgG (Fc gamma RII) and the 50- to 78-kDa receptor for the Fc portion of IgG (Fc gamma RIIIPMN) from human polymorphonuclear leukocytes. Fc gamma RIIIPMN isolated by both mAb and ligand affinity chromatography, but not Fc gamma RII, binds Con A in Western blots. This binding is specifically inhibitable by alpha-methylmannoside. Digestion of Fc gamma RIIIPMN by recombinant endoglycosidase H, which is specific for high mannose-type (Con A-binding) oligosaccharides, alters the epitope recognized by mAb 3G8 in or near the IgG ligand-binding site of the receptor. Similarly, the ability of Fc gamma RIIIPMN to bind human IgG ligand is sensitive to endoglycosidase H digestion. Our data indicate that ligands other than the classical IgG opsonins can bind to human Fc gamma RIIIPMN per se through lectin-carbohydrate interactions. Furthermore, Fc gamma RIIIPMN contains a high mannose type oligosaccharide chain which contributes importantly to the integrity of the classical IgG ligand-binding site. Thus, specific glycosylations of the receptor are important for both classical and nonclassical engagement of Fc gamma RIII and may play a role in determining the properties of the ligand-binding site.
Kimberly
R P
RP
Department of Medicine, Hospital for Special Surgery, New York 10021.
Tappe
N J
NJ
Merriam
L T
LT
Redecha
P B
PB
Edberg
J C
JC
Schwartzman
S
S
Valinsky
J E
JE
D000118:Acetylglucosaminidase; D000943:Antigens, Differentiation / Q000302:isolation & purification / Q000502:physiology*; D015153:Blotting, Western; D003208:Concanavalin A / Q000378:metabolism / Q000502:physiology; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D008358:Mannose / Q000378:metabolism / Q000502:physiology; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D009504:Neutrophils / Q000378:metabolism* / Q000502:physiology; D009844:Oligosaccharides / Q000502:physiology*; D011233:Precipitin Tests; D011961:Receptors, Fc / Q000302:isolation & purification / Q000502:physiology*; D017452:Receptors, IgG; D011975:Receptors, Mitogen / Q000502:physiology*; D013329:Structure-Activity Relationship
D000943:Antigens, Differentiation; D007074:Immunoglobulin G; D009844:Oligosaccharides; D011961:Receptors, Fc; D017452:Receptors, IgG; D011975:Receptors, Mitogen; D003208:Concanavalin A; D000118:Acetylglucosaminidase; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D008358:Mannose