Isolation and characterization of a mammalian gene encoding a high-affinity cAMP phosphodiesterase.
A rat brain cDNA library has been constructed in a Saccharomyces cerevisiae expression vector and used to isolate genes that can function in yeast to suppress the phenotypic effects of RAS2val19, a mutant form of the RAS2 gene analogous to an oncogenic mutant of the human HRAS gene. One cDNA, DPD, was cloned and its genetic and biochemical properties were characterized. A DPD product would share 80% amino acid sequence identity with the Drosophila melanogaster dunce-encoded protein over an extended region. We have shown that the DPD protein is a high-affinity cAMP-specific phosphodiesterase.
Colicelli
J
J
Cold Spring Harbor Laboratory, NY 11724.
Birchmeier
C
C
Michaeli
T
T
O'Neill
K
K
Riggs
M
M
Wigler
M
M
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D005816:Genetic Complementation Test; D006358:Hot Temperature; D008969:Molecular Sequence Data; D051381:Rats; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics
D004247:DNA; D015105:3',5'-Cyclic-AMP Phosphodiesterases