Glutaraldehyde in whole-cell Bordetella pertussis vaccine.
Treatment of the whole-cell bacterial suspensions of Bordetella pertussis with 0.05% glutaraldehyde at room temperature for 10 min kills the bacteria and, except for some histamine sensitising activity, almost detoxifies the pertussis toxin. The glutaraldehyde-vaccine is of good potency with a meritorious performance in tests for abnormal toxicity in mice, the leucocytosis-promoting-factor and the mouse weight gain. Using glutaraldehyde for the inactivation of whole-cell pertussis vaccine on a commercial scale must await extensive clinical trials with vaccine lots with a good record of safety and potency in animal tests.
Arya
S C
SC
King Fahd Central Hospital, Gizan, Kingdom of Saudi Arabia.
Ashraf
S J
SJ
Pathak
V P
VP
D000447:Aldehydes / Q000494:pharmacology*; D005976:Glutaral / Q000032:analysis / Q000494:pharmacology*; D010567:Pertussis Vaccine / Q000032:analysis* / Q000633:toxicity
D000447:Aldehydes; D010567:Pertussis Vaccine; D005976:Glutaral