PRP4 (RNA4) from Saccharomyces cerevisiae: its gene product is associated with the U4/U6 small nuclear ribonucleoprotein particle.
The PRP4 (RNA4) gene product is involved in nuclear mRNA processing in yeast cells; we have previously cloned the gene by complementation of a temperature-sensitive mutation. Sequence and transcript analyses of the cloned gene predicted the gene product to be a 52-kilodalton protein, which was confirmed with antibodies raised against the PRP4 gene product. These antibodies inhibited precursor mRNA splicing in vitro, demonstrating a direct role of PRP4 in splicing. Immunoprecipitations with the antibodies indicated that the PRP4 protein is associated with the U4/U6 small nuclear ribonucleoprotein particle.
Bjørn
S P
SP
Hospital for Sick Children, Toronto, Ontario, Canada.
Soltyk
A
A
Beggs
J D
JD
Friesen
J D
JD
D000595:Amino Acid Sequence; D000908:Antibodies, Fungal; D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics / Q000276:immunology; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D012326:RNA Splicing; D012261:Ribonucleoproteins / Q000235:genetics* / Q000276:immunology; D017411:Ribonucleoproteins, Small Nuclear; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic
D000908:Antibodies, Fungal; D004271:DNA, Fungal; D005656:Fungal Proteins; D012261:Ribonucleoproteins; D017411:Ribonucleoproteins, Small Nuclear