Stimulation of DNA chain initiation by a protein factor (NPF-1) from rat liver of different ages.
DNA Polymerase-alpha/primase complexes have been isolated from human neuroblastoma IMR-32, embryonic chicken brains (ECB) and rat prostate tumor PA-3 cells. In the presence of (NH4)2SO4 the major part (90%) of primase activity is released from the Pol-alpha/primase complex. A novel hydrophobic interaction column was used for purification of the primase from PA-3 cells. A nuclear protein factor (NPF-1) that stimulates DNA pol-alpha/primase activity has been purified from rat liver of various ages (3-6 months). The nuclear protein factor which only stimulates the primase activity is under investigation. The monoclonal antibodies (SJK 132-20 and 237-71) were used to detect DNA pol-alpha polypeptides from 11- to 19-day-old embryonic chicken brains.
Basu
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Department of Chemistry, University of Notre Dame, IN 46556.
Rosado
A T
AT
Takada
S
S
Ray
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Das
K
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Suzuki
I
I
Seve
A P
AP
D000375:Aging; D000546:Amanitins / Q000378:metabolism; D000818:Animals; D002843:Chromatin / Q000302:isolation & purification; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D004265:DNA Helicases / Q000378:metabolism; D019915:DNA Primase; D004261:DNA Replication; D004259:DNA-Directed DNA Polymerase / Q000097:blood / Q000378:metabolism; D008099:Liver / Q000201:enzymology* / Q000378:metabolism; D012316:RNA Nucleotidyltransferases / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism; D051381:Rats
D000546:Amanitins; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D019915:DNA Primase; D012316:RNA Nucleotidyltransferases; D004259:DNA-Directed DNA Polymerase; D004265:DNA Helicases