Analysis of human KS biopsies and cloned cell lines for cytomegalovirus, HIV-1, and other selected DNA virus sequences.
Investigation into a possible role of several human viruses, including human cytomegalovirus (HCMV), human immunodeficiency virus (HIV-1), hepatitis B virus (HBV), human herpesvirus 6 (HHV6), and Epstein-Barr virus (EBV) in the pathogenesis of Kaposi's sarcoma (KS) has resulted in the lack of an association of these viruses in KS biopsy and cloned KS cell line specimens. Since nearly all patients with KS, including those with HIV-associated KS, have a high incidence of HCMV infection, HCMV has been proposed to be etiologically associated with KS. Moreover, our previous studies showed the retention of HCMV morphological transforming region II (mtrII) in both transformed and tumor-derived cell lines. As a result, we focused on the nucleic acid hybridization analysis of both KS biopsies from AIDS patients as well as cloned KS endothelial cell lines for HCMV mtrII sequences. We also analyzed KS biopsy and KS cloned cell line specimens for HIV-1, HBV, HHV6, and EBV sequences, since these viruses have also been implicated in the etiology of KS. In one set of experiments, Southern blot analysis revealed the presence of HCMV mtrII sequences in two of six KS biopsies; in other experiments, HBV sequences were found in one of seven KS biopsies. No hybridization in any biopsy tissue was detected for HIV-1 DNA sequences. The analysis of six independently derived cloned KS cell lines was next studied. All these lines were negative for hybridization to the HCMV mtrII transforming fragment as well as to subgenomic fragments of HHV6 and EBV.(ABSTRACT TRUNCATED AT 250 WORDS)
Jahan
N
N
Department of Microbiology, Georgetown Medical Center, Washington, D.C. 20007.
Razzaque
A
A
Greenspan
J
J
Conant
M A
MA
Josephs
S F
SF
Nakamura
S
S
Rosenthal
L J
LJ
D015139:Blotting, Southern; D002460:Cell Line; D003587:Cytomegalovirus / Q000235:genetics; D004267:DNA Viruses / Q000235:genetics* / Q000502:physiology; D004279:DNA, Viral / Q000032:analysis*; D015497:HIV-1 / Q000235:genetics; D006515:Hepatitis B virus / Q000235:genetics; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans; D012514:Sarcoma, Kaposi / Q000382:microbiology*; D018139:Simplexvirus / Q000235:genetics
D004279:DNA, Viral