title: Characterization of a glycosyl-phosphatidylinositol degrading activity in Dictyostelium discoideum membranes.
abstract: Antigen 117 is a glycolipid-anchored cell surface protein implicated in cell-cell cohesion of Dictyostelium discoideum amoebae. Previous studies have demonstrated that during cell aggregation some of the protein is released from the cell surface. Here we report the characterization of the enzymatic activity involved in the 117 antigen release. The data indicate that the releasing enzyme is a phosphatidylinositol phospholipase C. The data also indicate that structural features of glycolipid anchors are conserved in a variety of organisms.
authors.affiliation: E. A. Doisy Department of Biochemistry and Molecular Biology, St. Louis, Missouri 63104.
authors.forename: A M
authors.initials: AM
authors.lastname: da Silva
authors.forename: C
authors.initials: C
authors.lastname: Klein
mesh_terms: D002413:Cations, Divalent; D002414:Cations, Monovalent; D015815:Cell Adhesion Molecules / Q000302:isolation & purification*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D004023:Dictyostelium / Q000378:metabolism*; D004229:Dithiothreitol / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D006017:Glycolipids / Q000378:metabolism*; D017261:Glycosylphosphatidylinositols; D007700:Kinetics; D008562:Membrane Glycoproteins / Q000302:isolation & purification*; D010618:Phenanthrolines / Q000494:pharmacology; D010716:Phosphatidylinositols / Q000378:metabolism*; D011480:Protease Inhibitors / Q000494:pharmacology
chemical_list: D002413:Cations, Divalent; D002414:Cations, Monovalent; D015815:Cell Adhesion Molecules; D006017:Glycolipids; D017261:Glycosylphosphatidylinositols; D008562:Membrane Glycoproteins; D010618:Phenanthrolines; D010716:Phosphatidylinositols; D011480:Protease Inhibitors; C054291:antigen 117, Dictyostelium; D004533:Egtazic Acid; D004492:Edetic Acid; D004229:Dithiothreitol; C025205:1,10-phenanthroline