The effect of the replacement of ADP with a photoaffinity ATP analogue, 2-azido-ADP, in F-actin on its function.
2-Azido-ATP, a photoaffinity ATP analogue, was incorporated into actin and the influence of the incorporation on the actin function was studied. The replacement of ADP with 2-azido-ADP in F-actin both before and after photocross-linking decreased appreciably the actin-activated S1-ATPase activity. Photocross-linked 2-azido-ADP-F-actin could be depolymerized by dialysis against a solution containing 0.1 mM CaCl2, 0.1 mM ATP and 1 mM Tris-HCl (pH 8.0). However, once it depolymerized, it lost very quickly the ability to polymerize even in the presence of a sufficient amount of ATP and Ca2+.
Kuwayama
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H
Laboratory of Chemistry, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
Miki
M
M
dos Remedios
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CG
D000199:Actins / Q000378:metabolism*; D000244:Adenosine Diphosphate / Q000031:analogs & derivatives* / Q000378:metabolism*; D000251:Adenosine Triphosphatases / Q000378:metabolism; D001386:Azides / Q000378:metabolism*; D003432:Cross-Linking Reagents; D010777:Photochemistry
D000199:Actins; D001386:Azides; D003432:Cross-Linking Reagents; D000244:Adenosine Diphosphate; C022295:2-azidoadenosine 3',5'-diphosphate; D000251:Adenosine Triphosphatases