Overproduction of transposon Tn10-encoded tetracycline resistance protein results in cell death and loss of membrane potential.
High-level expression of the Tn10 tetracycline resistance protein TetA in Escherichia coli caused partial collapse of the membrane potential, arrest of growth, and killing of the cells. Since alpha-methylglucoside transport was not affected, the overproduced TetA protein may cause not destruction of membrane structure but rather unrestricted translocation of protons and/or ions across the membrane.
Eckert
B
B
Institut für Biologie III, Albert-Ludwigs-Universität Freiburg, Federal Republic of Germany.
Beck
C F
CF
D001426:Bacterial Proteins / Q000502:physiology; D002470:Cell Survival; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D008564:Membrane Potentials; D008757:Methylglucosides / Q000378:metabolism; D011392:Proline / Q000378:metabolism; D013752:Tetracycline / Q000378:metabolism; D013753:Tetracycline Resistance
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D008757:Methylglucosides; C027020:methylglucoside; D011392:Proline; D013752:Tetracycline