A rapid method for isolation of human hemoglobin benzo[a]pyrene diol epoxide derived adducts using high performance liquid chromatography.
A method is described to isolate rapidly human hemoglobin-benzo[a]pyrene diol epoxide adducts. A combination of 300 A pore size C4 reversed phase HPLC to effect separation of adducted protein from native protein, and mu-bore C18 reversed phase HPLC to isolate and partially characterize proteolytic peptide adducts (by UV), was used.
Naylor
S
S
Department of Chemistry, Massachusetts Institute of Technology, Cambridge 02139.
Gan
L S
LS
Day
B W
BW
Skipper
P L
PL
Tannenbaum
S R
SR
D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; D002851:Chromatography, High Pressure Liquid; D004101:Dihydroxydihydrobenzopyrenes; D006454:Hemoglobins / Q000302:isolation & purification*; D006801:Humans; D015394:Molecular Structure
D004101:Dihydroxydihydrobenzopyrenes; D006454:Hemoglobins; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide