Stabilization of poliovirus against heat inactivation.
Fatty acids and related compounds, as well as many salts, stabilize poliovirus against heat inactivation. Addition of myristate to poliovirus prevents heat-induced conformational changes which are detected by trypsin degradation of the virion. Using equilibrium dialysis, we found that several molecules of myristate bind per virion. The relative stabilizing potencies of the salts can be explained by the Hofmeister effect.
Dorval
B L
BL
Department of Chemistry, Massachusetts Institute of Technology, Cambridge.
Chow
M
M
Klibanov
A M
AM
D005230:Fatty Acids, Nonesterified / Q000494:pharmacology*; D006367:HeLa Cells; D006358:Hot Temperature; D006801:Humans; D007700:Kinetics; D017955:Poliovirus / Q000502:physiology*; D013329:Structure-Activity Relationship; D013997:Time Factors
D005230:Fatty Acids, Nonesterified