Human non-transformed monocyte-derived macrophage cell lines.
We have demonstrated that human blood monocyte-derived macrophages can be passaged from primary cultures and replicate. Passaged cells have typical macrophage characteristics: they are non-specific esterase positive, phagocytic, and respond to 3 days of treatment with interferon-gamma with enhanced production of superoxide on stimulation with PMA. The passaged cells express Fc, CR1, CR3 and FMLP receptors. Both primary and passaged cultures constitutively produce CSF-1 after 3 weeks in culture. Cultures studied between 7 and 16 weeks in culture produce 3712 +/- 478 U of CSF-1 per 10(6) cells. Randomly selected lines were examined to look for cell proliferation by looking at numbers of cells over time and by labelling cells with tritiated thymidine to determine the number of cells synthesizing DNA. In addition, the cells can be frozen at the time of isolation and stored for at least 1 year, and then thawed and shown to retain functional activity. Human monocyte-derived macrophages can be cultured as finite cell lines.
Sechler
J M
JM
Bacterial Diseases Section, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892.
Warren
M K
MK
Gallin
J I
JI
D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases; D002455:Cell Division; D002460:Cell Line; D002470:Cell Survival; D002474:Cell-Free System; D002609:Chediak-Higashi Syndrome / Q000378:metabolism / Q000473:pathology; D003115:Colony-Stimulating Factors / Q000032:analysis; D005615:Freezing; D006105:Granulomatous Disease, Chronic / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008264:Macrophages / Q000201:enzymology / Q000473:pathology / Q000502:physiology; D009000:Monocytes / Q000502:physiology; D010587:Phagocytosis; D011951:Receptors, Complement / Q000172:deficiency; D017463:Receptors, Complement 3b
D003115:Colony-Stimulating Factors; D011951:Receptors, Complement; D017463:Receptors, Complement 3b; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase