New method of photosensitizer accumulation for photodynamic therapy in an experimental liver tumor.
A new sensitizing method of photodynamic therapy for malignant tumors and its effects was studied. Prepared for the study were pheophorbide a (Phd), dissolved in an oily contrast medium, Lipiodol (LPD-Phd), and water-soluble pheophorbide a (W-S Phd) as sensitizers, and VX-2 tumor in rabbit livers. The Phd distribution was compared after intraarterial (i.a.) administration of LPD-Phd or W-S Phd and intravenous (i.v.) administration of W-S Phd. Phd was extracted with methanol at 24 h after injection, and the supernatant absorbance was measured at 670 nm by spectrophotometry. The tumor showed higher values of Phd than did the liver with LPD-Phd i.a. and W-S Phd i.a. (P less than .01). Conversely, the tumor accumulated less Phd than did the liver with W-S Phd i.v. (P less than .05). We subsequently produced severe photo-destruction in a Walker tumor in a Sprague-Dawley rat liver with slight damage to adjacent liver tissue using LPD-Phd i.a. and Nd-YAG dye laser irradiation at 670 nm. The intraarterial administration of a photosensitizer may make it possible to treat liver tumors by photodynamic therapy.
Nishiwaki
Y
Y
Second Department of Surgery, Hamamatsu University School of Medicine, Japan.
Nakamura
S
S
Sakaguchi
S
S
D000818:Animals; D002734:Chlorophyll / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D007459:Iodized Oil / Q000008:administration & dosage / Q000493:pharmacokinetics; D007834:Lasers; D008099:Liver / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy*; D008297:Male; D010778:Photochemotherapy / Q000379:methods*; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains
D002734:Chlorophyll; D007459:Iodized Oil; C032623:pheophorbide a