Hypothyroidism in rats decreases mitochondrial inner membrane cation permeability.
We investigated the cation permeability of liver mitochondria isolated from hypothyroid or euthyroid rats by measuring the rate of swelling of respiring mitochondria in acetate salts as a function of membrane potential. Mitochondria from hypothyroid rats have a decreased permeability of roughly 3-fold in the presence of monovalent cations K and tetramethylammonium at any (measured) membrane potential. Since the monovalent cation leak and the proton leak are known to respond similarly to membrane potential our results support the theory that the difference in non-phosphorylating respiration rate between mitochondria from hypothyroid and euthyroid rats is due to a difference in proton leak.
Hafner
R P
RP
Department of Biochemistry, University of Cambridge, England.
Leake
M J
MJ
Brand
M D
MD
D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid; D000818:Animals; D007037:Hypothyroidism / Q000378:metabolism*; D007425:Intracellular Membranes / Q000378:metabolism*; D008564:Membrane Potentials; D008928:Mitochondria / Q000378:metabolism*; D008933:Mitochondrial Swelling; D010101:Oxygen Consumption; D010766:Phosphorylation; D011522:Protons; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D051381:Rats
D000085:Acetates; D011522:Protons; D000644:Quaternary Ammonium Compounds; C027917:tetramethylammonium; D019342:Acetic Acid