Content, distribution and stability of protein-synthesis elongation factor Tu in subcellular fractions of vegetative cells and spores of Streptomyces aureofaciens.
The protein synthesis elongation factor Tu (EF-Tu) was identified in dormant spores of Streptomyces aureofaciens and its content and distribution in vegetative cells and dormant spores were determined. Cell-free homogenates from spores were found to contain a EF-Tu cleaving membrane bound protease. The protease cleaved aggregated EF-Tu much less efficiently than non-aggregated factor in cell homogenates. The relative content of EF-Tu and ribosomes in dormant spores was very similar to that found in exponentially growing vegetative cells.
Weiser
J
J
Institute of Microbiology, Czechoslovak Academy of Sciences, Prague.
Morava
P
P
Vohradský
J
J
D002462:Cell Membrane / Q000201:enzymology; D003593:Cytoplasm / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D010444:Peptide Elongation Factor Tu / Q000032:analysis* / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D012270:Ribosomes / Q000032:analysis; D013171:Spores, Bacterial / Q000032:analysis / Q000201:enzymology; D013304:Streptomyces aureofaciens / Q000032:analysis* / Q000201:enzymology / Q000378:metabolism; D013997:Time Factors
D010447:Peptide Hydrolases; D010444:Peptide Elongation Factor Tu