title: Immunoelectron microscopic localization of the F1F0 ATPase (ATP synthase) on the cytoplasmic membrane of alkalophilic Bacillus firmus RAB.
abstract: Evidence that the F1F0 ATPase (ATP synthase) of alkalophilic Bacillus firmus RAB is localized exclusively on the cytoplasmic membrane was obtained by immunogold electron microscopy using a highly specific polyclonal antibody against the beta subunit of Escherichia coli F1F0 ATPase. The energetic problem faced by cells of B. firmus RAB growing oxidatively at pH 10.5 despite a low protonmotive force across the cytoplasmic membrane cannot, therefore, be circumvented by localization of energy transducing functions on hypothetical internal membranes.
authors.affiliation: Gesellschaft für Biotechnologische Forschung, Braunschweig, F.R.G.
authors.forename: M
authors.initials: M
authors.lastname: Rohde
authors.forename: F
authors.initials: F
authors.lastname: Mayer
authors.forename: D B
authors.initials: DB
authors.lastname: Hicks
authors.forename: T A
authors.initials: TA
authors.lastname: Krulwich
mesh_terms: D001407:Bacillus / Q000201:enzymology* / Q000648:ultrastructure; D002462:Cell Membrane / Q000201:enzymology* / Q000648:ultrastructure; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D006180:Proton-Translocating ATPases / Q000032:analysis*
chemical_list: D006180:Proton-Translocating ATPases