Phosphorylation of vesicular stomatitis virus M protein: evidence for a second virion-associated protein serine kinase activity.
The vesicular stomatitis virus (VSV) NS and M proteins are not only phosphorylated in vivo but are also further modified by the virion-associated protein kinase(s) concomitantly with the in vitro transcription process. Although NS phosphorylation is necessary for this transcription, no function has yet been ascribed for M protein phosphorylation. We show here that all phosphates added to M protein in vitro mapped to the trypsin-sensitive N-terminal basic domain (residues 1-43). The major site(s) (approximately 93%) corresponded to one or more of three serine residues within the first 17 amino acids. Nearly 1 mol phosphate/mol protein was added in vitro under optimal conditions suggesting that only one of these three candidate serine residues corresponds to the major site. This same M protein domain is thought to play an important role in virus RNA synthesis by inhibiting transcription. We show here that in vitro phosphorylation did not appear to affect this function. Two critical serine residues in the VSV NS protein were previously reported to be phosphorylated during in vitro transcription (D. Chattopadhyay and A. K. Banerjee, 1987, Cell 49, 407-414). The sequence flanking these NS serines is very acidic while that of all three candidate phosphoserines in the M protein is very basic. We therefore predict that at least two distinct serine-specific kinase activities are packaged in virions, one specific for M and one specific for NS.
Beckes
J D
JD
Department of Biology, San Diego State University, California 92182.
Childers
L C
LC
Perrault
J
J
D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D002213:Capsid / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D004279:DNA, Viral / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases; D012694:Serine / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D013379:Substrate Specificity; D014158:Transcription, Genetic; D014721:Vesicular stomatitis Indiana virus / Q000201:enzymology* / Q000235:genetics; D014758:Viral Core Proteins / Q000235:genetics / Q000378:metabolism; D014763:Viral Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D017361:Viral Nonstructural Proteins; D014771:Virion / Q000201:enzymology / Q000235:genetics
D004279:DNA, Viral; D014758:Viral Core Proteins; D014763:Viral Matrix Proteins; D017361:Viral Nonstructural Proteins; D012965:Sodium Chloride; D012694:Serine; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases