A sensitive enzyme immunoassay for atrial natriuretic polypeptide.
A sensitive sandwich enzyme immunoassay for mammalian alpha-atrial natriuretic polypeptide (alpha-ANP) has been developed using Fab' fragments of affinity purified rabbit anti-alpha-hANP[6-28] polyclonal antibodies (R3) conjugated to horseradish peroxidase. Immunoplates were coated with (F(ab')2) fragments of anti-alpha-hANP monoclonal antibodies (HA53). Without using a pre-concentration step the detection limit of alpha-hANP in plasma was 5 pg/ml. The assay was sufficient accuracy and reproducibility for general use of, i.e., the coefficients of within-assay and between-assay variation were 6.4-10.7% and 9.7-15.3%, respectively. Using the assay, the basal plasma alpha-hANP level of healthy persons was 15.3 +/- 8.3 pg/ml (mean +/- SD, n = 40).
Watanabe
H
H
Chemistry Research Laboratories, Takeda Chemical Industries, Ltd., Osaka, Japan.
Wakimasu
M
M
Kondo
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D000918:Antibody Specificity; D009320:Atrial Natriuretic Factor / Q000097:blood* / Q000276:immunology; D001667:Binding, Competitive; D006801:Humans; D007124:Immunoenzyme Techniques; D010446:Peptide Fragments / Q000032:analysis; D051381:Rats
D000911:Antibodies, Monoclonal; D010446:Peptide Fragments; D009320:Atrial Natriuretic Factor