Thyrotropin-releasing hormone effects on phosphatidylinositol metabolism and binding in rat pituitary and retina.
This paper reveals possible similarities in the thyrotropin-releasing hormone (TRH) effects on phospholipid metabolism in pituitary and retina of the rat central nervous system. Addition of the methylated analog (MeTRH) resulted in 171 +/- 16% and 88 +/- 10% increase in 32PO4 incorporation into phosphatidylinositol (PI) in pituitary (20 min incubation) and retina (60 min incubation), respectively. There was a similar significant increase in phosphatidic cid, but with no change in phosphatidylcholine or other classes of phospholipids. The effect was concentration-dependent and the ED50 also was close to KD, suggesting the response was regulated by MeTRH receptors in membranes of both pituitary and retina.
Naseem
S M
SM
University of Maryland, School of Medicine, Baltimore 21201.
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D005260:Female; D066298:In Vitro Techniques; D007700:Kinetics; D010710:Phosphates / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D010903:Pituitary Gland, Anterior / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D017981:Receptors, Neurotransmitter / Q000187:drug effects / Q000378:metabolism; D018025:Receptors, Thyrotropin-Releasing Hormone; D012160:Retina / Q000187:drug effects / Q000378:metabolism*; D013973:Thyrotropin-Releasing Hormone / Q000097:blood* / Q000378:metabolism
D010710:Phosphates; D010716:Phosphatidylinositols; D010743:Phospholipids; D017981:Receptors, Neurotransmitter; D018025:Receptors, Thyrotropin-Releasing Hormone; D013973:Thyrotropin-Releasing Hormone