Discrimination between twenty isolates of herpesvirus simiae (B virus) by restriction enzyme analysis of the viral genome.
Twenty isolates, obtained from adult breeding monkeys, were all identified as herpesvirus simiae (B virus) by neutralisation with polyclonal B virus antiserum. Subsequent analysis of restriction enzyme profiles produced by digestion of DNA from the isolates enabled discrimination to be made between them. In particular Cynomolgus monkey isolates could be distinguished from those of Rhesus animals. One isolate (isolate 9) could not be typed either as B virus or as the antigenically related herpesvirus SA8, despite neutralisation by B virus antiserum. Unlike herpes simplex virus, B virus isolates could not be divided into oral and genital types on the basis of restriction enzyme profiles.
Wall
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Chemical Defence Establishment, Porton Down, Salisbury, U.K.
Zwartouw
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HT
Kelly
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DC
D000818:Animals; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005814:Genes, Viral; D006564:Herpesviridae / Q000145:classification*; D006568:Herpesvirus 1, Cercopithecine / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008252:Macaca fascicularis / Q000382:microbiology; D008253:Macaca mulatta / Q000382:microbiology; D008297:Male; D009500:Neutralization Tests; D012703:Serotyping
D004279:DNA, Viral; D004262:DNA Restriction Enzymes