Isolation and culture of HCO3- -secreting intercalated cells.
Intercalated cells of the distal nephron secrete either H+ or HCO3-. We have succeeded in isolating HCO3- -secreting intercalated cells from the rabbit kidney. When seeded onto collagen-coated permeable supports, these cells form monolayers with a resistance of 595 +/- 75 omega.cm2. The monolayers maintain the characteristics of an epithelium, with apical microvillae and tight junctions. They display the same polarity as do HCO3- -secreting intercalated cells in vivo, namely apical peanut lectin binding and apical Cl- -HCO3- exchange. The monolayers are capable of transepithelial HCO3- transport via this exchanger. The rate of Cl- -dependent transepithelial HCO3- transport is 4 +/- 0.4 nmol.min-1.cm-2. Transepithelial HCO3- transport is completely abolished by 50 microM 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid applied to the apical side of the monolayer. These cultured HCO3- -secreting intercalated cells should prove useful for defining the cellular regulation of HCO3- secretion.
Van Adelsberg
J
J
Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, New York 10032.
Edwards
J C
JC
Herzlinger
D
D
Cannon
C
C
Rater
M
M
al-Awqati
Q
Q
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D001639:Bicarbonates / Q000378:metabolism*; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D002712:Chlorides / Q000378:metabolism; D007684:Kidney Tubules / Q000166:cytology*; D007685:Kidney Tubules, Collecting / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D011817:Rabbits; D015222:Sodium Channels / Q000502:physiology
D001639:Bicarbonates; D002712:Chlorides; D015222:Sodium Channels; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid