The role of suppressor factors in the regulation of immune responses by ultraviolet radiation-induced suppressor T lymphocytes. I. Activity of suppressor cell culture supernatants.
The purpose of this study was to determine whether soluble suppressor factors are involved in the regulation of immune responses by ultraviolet radiation-induced suppressor T lymphocytes (UV Ts). The UV Ts were induced by applying contact allergens to the ventral, unirradiated skin of mice that had been exposed 5 days earlier to UVB radiation. Supernatants from cultures that contained a mixture of UV Ts, normal responder lymphocytes, and hapten-modified stimulator cells were injected iv into normal recipients at the time of sensitization; they inhibited the induction of contact hypersensitivity (CHS) in vivo in an hapten-specific manner. The supernatants similarly suppressed the generation of specific cytotoxic T lymphocytes (CTL) in vitro. Moreover, supernatants from cultures that contained either UV Ts alone or UV Ts in combination with either the responder or the stimulator cells failed to suppress the CHS and CTL responses. These results suggest that hapten-specific inhibitory factors may participate in the regulation of immune responses by suppressor cells generated by epicutaneous sensitization of UV-irradiated mice.
Yee
G K
GK
Department of Immunology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Ullrich
S E
SE
Kripke
M L
ML
D000818:Animals; D002478:Cells, Cultured; D003877:Dermatitis, Contact / Q000276:immunology; D005260:Female; D051379:Mice; D008809:Mice, Inbred C3H; D013491:Suppressor Factors, Immunologic / Q000096:biosynthesis / Q000502:physiology*; D013602:T-Lymphocytes, Cytotoxic; D050378:T-Lymphocytes, Regulatory / Q000378:metabolism / Q000528:radiation effects*; D014466:Ultraviolet Rays
D013491:Suppressor Factors, Immunologic