[Molecular biology techniques for detection of delta hepatitis virus. I: Obtaining the specific diagnostic probe].
A specific probe to detect DHV genomes was developed using as a source the p alpha-1 recombinant plasmid. The probe was labelled by using the non-isotopic method which allows its longer storage. The sensitivity of this DHV probe varies between 1 to 5 pcg.
Toro
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Seccion de Biología Molecular, Centro Nacional de Referencia en Inmunología Clínica.
Ramírez
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JL
Bianco
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NE
Machado
I V
IV
D015342:DNA Probes; D004587:Electrophoresis, Agar Gel; D016679:Genome, Viral; D003699:Hepatitis D / Q000175:diagnosis* / Q000235:genetics; D003698:Hepatitis Delta Virus / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization; D010957:Plasmids / Q000235:genetics
D015342:DNA Probes
Técnicas de biología molecular para la detección del virus de hepatitis delta I: obtención de la sonda diagnóstica específica.