title: Myocardial (iso)enzyme activities, DNA concentration and nuclear polyploidy in hearts of patients operated upon for congenital heart disease, and in normal and hypertrophic adult human hearts at autopsy.
abstract: To investigate biochemical characteristics of hypertrophic myocardium of young and adult humans, we analysed myocardial biopsies obtained from 28 mainly young patients undergoing cardiac surgery for congenital heart disease and 41 autopsied hearts from 18 adult normal and 23 hypertrophic human subjects. Myocardial activities of the enzymes creatine kinase and lactate dehydrogenase were independent of age during childhood, but decreased significantly with hypertrophy at adult age. Myocyte nuclei showed increased polyploidization during childhood which was progressive with age, and in the adult stage polyploidization was correlated with heart weight. Nevertheless myocardial DNA concentration fell under both conditions, which is to be ascribed to the 'diluting' effect of myocyte hypertrophy. Before an age of 8 years DNA concentration in the child heart material studied has reached the value found in adult nonhypertrophic hearts, although at that time polyploidization of myocyte nuclei in child hearts was only half the value found in adult non-hypertrophic hearts. Biochemical measurement of DNA concentration in peroperatively taken myocardial biopsies may contribute to the in vivo diagnosis of ventricular hypertrophy in quantitative terms, in combination with radiology, echocardiography and histology.
authors.affiliation: Department of Cardiology, University Hospital Leiden, The Netherlands.
authors.forename: A
authors.initials: A
authors.lastname: van der Laarse
authors.forename: L
authors.initials: L
authors.lastname: Hollaar
authors.forename: H W
authors.initials: HW
authors.lastname: Vliegen
authors.forename: J M
authors.initials: JM
authors.lastname: Egas
authors.forename: N J
authors.initials: NJ
authors.lastname: Dijkshoorn
authors.forename: C J
authors.initials: CJ
authors.lastname: Cornelisse
authors.forename: A J
authors.initials: AJ
authors.lastname: Bogers
authors.forename: J M
authors.initials: JM
authors.lastname: Quaegebeur
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D006332:Cardiomegaly / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002467:Cell Nucleus / Q000648:ultrastructure; D002648:Child; D002675:Child, Preschool; D003402:Creatine Kinase / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D005260:Female; D006330:Heart Defects, Congenital / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007223:Infant; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D009929:Organ Size; D011123:Polyploidy
chemical_list: D007527:Isoenzymes; D004247:DNA; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase