Characterization of beta-adrenergic receptors in cultured human trabecular cells and in human trabecular meshwork.
The characterization of beta-adrenergic receptors on cultured human trabecular cells and trabecular meshwork from human autopsy eyes was carried out by radioligand binding utilizing (125I)-iodopindolol. In cultured cells, the observed binding of (125I)-iodopindolol was of high affinity (Kd = 43 pM) and saturable. Scatchard plots were linear and revealed a Bmax of 33 +/- 7 fmol/mg of protein. Competition studies with a series of agonists and antagonists revealed that human trabecular cells contain a single class of beta-adrenergic receptors of the beta 2 subtype. Similarly, the IC50 of ICI 89,406 (176 nM) in human trabecular meshwork from autopsy eyes supports the presence of beta 2-adrenergic receptors in this tissue.
Wax
M B
MB
Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia 19104-6084.
Molinoff
P B
PB
Alvarado
J
J
Polansky
J
J
D000328:Adult; D000368:Aged; D001665:Binding Sites; D002478:Cells, Cultured; D006801:Humans; D008875:Middle Aged; D010869:Pindolol / Q000031:analogs & derivatives / Q000378:metabolism; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D014129:Trabecular Meshwork / Q000166:cytology / Q000378:metabolism*
D011943:Receptors, Adrenergic, beta; C029247:3-iodopindolol; D010869:Pindolol