Production and characterisation of immunoreactive calcitonin gene-related peptide (CGRP) from a CGRP receptor-positive cloned osteosarcoma cell line (UMR 106.01).
A subclone of an osteoblast-like osteosarcoma cell line (UMR 106.01) has recently been shown to possess specific binding sites for calcitonin gene-related peptide (CGRP) linked to adenylate cyclase. The present study provides the first demonstration for the production of immunoreactive CGRP from CGRP-receptor positive osteosarcoma cells. Mean immunoreactive CGRP levels were 15 pmol/g and 1 pmol/l for acid extracts of cells and cell-exposed media respectively. On gel filtration and high performance liquid chromatography, a major proportion of immunoreactive CGRP was found to co-elute with synthetic rat CGRP(1-37). Only negligible quantities of calcitonin were detected in cell extracts or cell-exposed supernatant. The production of authentic CGRP from a CGRP-receptor positive tumour suggests that the peptide may have autocrine effects on its producer cell.
Zaidi
M
M
Department of Chemical Pathology, Royal Postgraduate Medical School, London, UK.
Datta
H K
HK
Chambers
T J
TJ
MacIntyre
I
I
D000818:Animals; D001667:Binding, Competitive; D002116:Calcitonin / Q000378:metabolism*; D015740:Calcitonin Gene-Related Peptide; D002460:Cell Line; D002850:Chromatography, Gel; D002999:Clone Cells; D009479:Neuropeptides / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification; D012516:Osteosarcoma; D011863:Radioimmunoassay; D051381:Rats; D018003:Receptors, Calcitonin; D011956:Receptors, Cell Surface / Q000032:analysis*
D009479:Neuropeptides; D018003:Receptors, Calcitonin; D011956:Receptors, Cell Surface; D002116:Calcitonin; D015740:Calcitonin Gene-Related Peptide