Poly[N-acetyl-lactosamine]-type sugar chains in CD45 antigens of abnormal T cells of lpr mice are different from those of normal T cells and B cells.
The lymph node (LN) T cells from autoimmune MRL/MpJ-lpr/lpr (lpr) mice and control MRL/MpJ-+/+ (+/+) mice were compared as to their cell surface lectin binding sites and the glycoproteins responsible for the lectin binding. T cells from enlarged lymph nodes of lpr mice were found to express more binding sites for lectins which are reactive to poly[N-acetyl-lactosamine]-type sugar chains than normal +/+ mouse lymph node T cells. Furthermore, we found that high mol. wt (180,000-220,000) glycoproteins on lpr T cells were strongly stained with these poly [N-acetyl-lactosamine]-binding lectins on Western-blotting. These glycoproteins were found to belong to the CD45 family on immunoprecipitation and absorption with monoclonal anti-CD45 antibody. Thus, aberrant expression of high mol. wt CD45 (CD45R) antigens on lpr T cells may contribute greatly to the strong reaction of these cells with poly[N-acetyl-lactosamine]-binding lectins. We also found that poly[N-acetyl-lactosamine]-type sugar chains are more abundant on B cells than on lpr T cells, and that the molecular weights and the carbohydrate moieties of CD45R antigens on lpr T cells are different from those of CD45R antigens on +/+ spleen B cells.
Yamashita
Y
Y
Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Imai
Y
Y
Osawa
T
T
D000818:Animals; D000943:Antigens, Differentiation / Q000032:analysis*; D001327:Autoimmune Diseases / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D006026:Glycoside Hydrolases; D006649:Histocompatibility Antigens / Q000032:analysis*; D037102:Lectins / Q000378:metabolism; D017493:Leukocyte Common Antigens; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000032:analysis*; D051379:Mice; D008815:Mice, Inbred Strains; D011134:Polysaccharides / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology*; D001616:beta-Galactosidase / Q000494:pharmacology
D000943:Antigens, Differentiation; D006649:Histocompatibility Antigens; D037102:Lectins; D008562:Membrane Glycoproteins; D011134:Polysaccharides; C037199:poly-N-acetyllactosamine; D017493:Leukocyte Common Antigens; D006026:Glycoside Hydrolases; C026093:keratan-sulfate endo-1,4-beta-galactosidase; D001616:beta-Galactosidase