Biochemical characterization of genotypes at the phosphoglucomutase-2 locus in the Pacific oyster, Crassostrea gigas.
The four most common allozymes at the Pgm-2 locus in Crassostrea gigas were purified and characterized over physiological ranges of temperature and pH. Significant differences were observed between genotypes in their apparent Michaelis constants for glucose-1-phosphate and glucose-1,6-diphosphate, V max/Km ratios, pH-dependent activities, and temperature stabilities. These functional differences were caused almost exclusively by the divergent properties of the Pgm-292 allozyme; limited differentiation existed among the Pgm-296, Pgm-2100, and Pgm-2104 variants. Heterozygotes displayed strict intermediacy for all kinetic and structural properties examined. The results are discussed in light of their ability to account for the overdominant body weights of Pgm-2 heterozygotes reported by Fujio (1982). It is concluded that overdominance is unlikely to arise at this locus as a consequence of these biochemical differences because of their limited magnitude and incompatibility with allelic frequencies in natural populations.
Pogson
G H
GH
Department of Zoology, University of British Columbia, Vancouver, Canada.
D000483:Alleles; D000818:Animals; D004592:Electrophoresis, Starch Gel; D005787:Gene Frequency; D005796:Genes; D005838:Genotype; D007527:Isoenzymes / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D010125:Ostreidae / Q000201:enzymology / Q000235:genetics*; D010733:Phosphoglucomutase / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D013816:Thermodynamics
D007527:Isoenzymes; D010733:Phosphoglucomutase