title: Amino acid sequence of the variable domains of a human anti-Rh(c) antibody: presence of an unusually long CDR3 in the lambda chain.
abstract: The complete amino acid sequence of the lambda light chain and the variable domain of the heavy chain of an anti-Rh(c) human monoclonal antibody were determined. The lambda chain presents a long third complementarity-determining region sequence with unusual amino acid insertions at the C-terminus. The proposed sequence indicates that this lambda chain may be assigned to the variable region subgroup I. The J segment is identical to that of J lambda 2 except for the first amino acid residue. Positions 152 (serine) and 190 (arginine) from this sequence correspond to the Kern-Oz- isotype, respectively. The VH segment can be classified as a VHIII subgroup member. The CDR1 segment of the anti-Rh(c) VH region has the same sequence as the VH of human BRO protein except for the first residue of the CDR1. The amino acid sequence of the anti-Rh(c) D segment does not match any published D segment. The JH segment used in this protein can be classified as a JH3 with a single amino acid difference at the fourth residue.
authors.affiliation: Laboratoire de Biologie Moléculaire des Récepteurs, CNRS, Université Paris VII, France.
authors.forename: L
authors.initials: L
authors.lastname: Chouchane
authors.forename: J L
authors.initials: JL
authors.lastname: Guillaume
authors.forename: D
authors.initials: D
authors.lastname: Goossens
authors.forename: P
authors.initials: P
authors.lastname: Rouger
authors.forename: A D
authors.initials: AD
authors.lastname: Strosberg
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D002460:Cell Line; D006801:Humans; D007135:Immunoglobulin Variable Region; D007146:Immunoglobulin lambda-Chains; D007518:Isoantibodies; D008969:Molecular Sequence Data
chemical_list: D000911:Antibodies, Monoclonal; D007135:Immunoglobulin Variable Region; D007146:Immunoglobulin lambda-Chains; D007518:Isoantibodies; C033242:RH-antibodies