Role of the proline residues on the immunogenic properties of a P. falciparum circumsporozoite peptide linked to a carrier protein.
The circumsporozoite (CS) protein of P. falciparum contains an immunodominant epitope, NADP, that is repeated 37 times in the native molecule. The presence of proline in the coat proteins of the Plasmodium parasite at various developmental stages and strains is a frequent occurrence. In this study we evaluate the influence of substitution of proline residues by glycine on the immunogenic behavior of two tandemly repeated peptides linked via glutaraldehyde to a protein carrier: The (NANP)4 P. falciparum circumsporozoite peptide and its glycine-substitute analog, (NANG)4. The results obtained show that the (NANP)4 induces antibodies which recognize the peptide free in solution, bound on a solid phase, and linked to a carrier protein. It has been previously reported that such antibodies recognize the antigenic sites of the peptide in the native protein on the surface of the sporozoite. Antibodies raised against (NANG)4 in the same experimental conditions as (NANP)4, cannot recognize the peptide free in solution or bound to the solid phase. However, these antibodies can react with the peptide when it is linked to a protein carrier. The coupling of a glycine-containing analog to a carrier results in a significant shift in its conformation, allowing it to be recognized by the antibodies.
Lise
L D
LD
University of South Florida.
Jolivet
M
M
Audibert
F
F
Fernandez
A
A
Wickstrom
E
E
Chedid
L
L
Schlesinger
D H
DH
D000818:Animals; D000913:Antibodies, Protozoan; D000936:Antigen-Antibody Complex; D000953:Antigens, Protozoan / Q000276:immunology* / Q000378:metabolism; D001665:Binding Sites; D002352:Carrier Proteins / Q000378:metabolism*; D002942:Circular Dichroism; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D010963:Plasmodium falciparum / Q000276:immunology*; D011392:Proline; D011487:Protein Conformation; D015800:Protozoan Proteins / Q000276:immunology*; D013329:Structure-Activity Relationship
D000913:Antibodies, Protozoan; D000936:Antigen-Antibody Complex; D000953:Antigens, Protozoan; D002352:Carrier Proteins; D015800:Protozoan Proteins; C041595:circumsporozoite protein, Protozoan; D011392:Proline