Role of the cysteine-rich region of HIV tat protein on its trans-activational ability.
The tat protein of human immunodeficiency virus (HIV) has a characteristic cysteine-rich region containing 7 cysteines within 16 residues. The role of this region was investigated by creation of several tat gene mutants. The activities of the novel tat gene translational products were assayed by measuring the co-transfected chloramphenicol acetyl-transferase (CAT) gene expression controlled by HIV long-terminal repeat (LTR) in the COS 7 cells. Substitution of either Cys22 with Gly, or Cys34-Gln-Val-Cys with His-Gln-Val-His, and deletion behind Lys50 of the tat protein caused a drastic loss in its activity.
Kubota
S
S
Institute for Virus Research, Kyoto University, Japan.
Endo
S
S
Maki
M
M
Hatanaka
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D003545:Cysteine / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D005091:Exons; D005786:Gene Expression Regulation; D015696:Gene Products, tat; D005814:Genes, Viral; D006678:HIV / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids; D014157:Transcription Factors / Q000235:genetics* / Q000502:physiology; D054322:tat Gene Products, Human Immunodeficiency Virus
D004279:DNA, Viral; D015696:Gene Products, tat; D014157:Transcription Factors; D054322:tat Gene Products, Human Immunodeficiency Virus; D015500:Chloramphenicol O-Acetyltransferase; D003545:Cysteine