Characterization of siderophores from Ustilago maydis.
Two siderophores, ferrichrome and ferrichrome A, were found in cultures of Ustilago maydis (DC) Corda. Both siderophores were found intracellularly and extracellularly. Their authenticity was confirmed by thin layer chromatography, HPLC, UV-visible spectrometry, paper electrophoresis, amino acid analysis, NMR and fast atom bombardment mass spectroscopy. Regulation of siderophore production by iron was examined. Repression of biosynthesis of extracellular siderophores occurred at 10(-5) M iron. Ferrichrome was found intracellularly at all iron concentrations employed; in general, ferrichrome A was not found to be cell-associated.
Budde
A D
AD
USDA/ARS, Plant Disease Resistance Research Unit, Department of Plant Pathology, University of Wisconsin, Madison 53706.
Leong
S A
SA
D000535:Aluminum; D000596:Amino Acids / Q000032:analysis; D001487:Basidiomycota / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004590:Electrophoresis, Paper; D005291:Ferrichrome / Q000302:isolation & purification; D007502:Iron Chelating Agents / Q000032:analysis*; D017262:Siderophores; D014588:Ustilago / Q000032:analysis*
D000596:Amino Acids; D007502:Iron Chelating Agents; D017262:Siderophores; D005291:Ferrichrome; D000535:Aluminum