The effect of phospholipase A2 and cyclooxygenase inhibition on free fatty acids in the brain.
The levels of NEFA (oleic acid, palmitic acid, stearic acid, arachidonic acid) were studied in the brain cortex, hypothalamus and the brain stem of rat brain. Inhibition of phospholipase A2 by Delagil (Mepacrinium dichloratum CsL 3) decreased statistically significantly the values of arachidonic aci (-5.5%) in the brain cortex and the values of stearic acid (-11.2%) in the hypothalamua. The cyclooxygenase inhibition by aspirin led to a significant increase of palmitic acid (+12.7%), oleic acid (+18.7%) and arachidonic acid (+14.3%) in the hypothalamus. Total NEFA increased by 11.2%. A significant increase of palmitic acid (+26.9%), arachidonic acid (+33.9%) and of total NEFA (+22.9%) was evidenced in the brain stem. Delagil administered in combination with acetylsalicylic acid suppressed the NEFA increase observed after the application of acetylsalicylic acid alone. A statistically significant decrease was found in stearic acid (-12.6%) in the hypothalamus and in all studied NEFA in the brain stem (palmitic acid, -14.2% stearic acid -11.7%, oleic acid -3.8%, arachidonic acid -16.6% and total NEFA -11.8%).
Sklenovský
A
A
Navrátil
J
J
Chmela
Z
Z
D000818:Animals; D001241:Aspirin / Q000494:pharmacology; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005227:Fatty Acids / Q000378:metabolism*; D008297:Male; D010740:Phospholipases / Q000502:physiology*; D010741:Phospholipases A / Q000378:metabolism / Q000502:physiology*; D054467:Phospholipases A2; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains
D004791:Enzyme Inhibitors; D005227:Fatty Acids; D011451:Prostaglandin-Endoperoxide Synthases; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D001241:Aspirin