Inhibitory effect of dibutyryl cyclic adenosine monophosphate on the induction of alkaline phosphatase in human fetal liver cell line.
When human fetal liver cells (HuL-1-317), cultured continuously in a serum-free medium, were incubated with a combination of prednisolone, butyrate and a hypertonic concentration of NaCl at 37 degrees C, alkaline phosphatase activity increased. However, the addition of dibutyryl adenosine cyclic monophosphate (Bt2cAMP) to these agents inhibited the increase in alkaline phosphatase activity in a dose-dependent manner: the inhibitory effect of Bt2cAMP was significant at 0.05 mM, but disappeared at 0.01 mM. Both cycloheximide and actinomycin D inhibited the increase in alkaline phosphatase activity with the combination described above. Western blotting showed that this enzyme activity increase was a consequence of greater biosynthesis of enzyme molecules in HuL-1-317 cells, and that Bt2cAMP regulated the synthesis of enzyme molecules. We conclude that the changes in alkaline phosphatase activity under various conditions are based on the changes in the number of enzyme molecules in HuL-1-317 cells.
Sorimachi
K
K
Department of Microbiology, Dokkyo University School of Medicine, Tochigi, Japan.
Ieiri
T
T
Kobayashi
M
M
Horiuchi
R
R
Yasumura
Y
Y
D000469:Alkaline Phosphatase / Q000096:biosynthesis*; D000818:Animals; D015153:Blotting, Western; D003994:Bucladesine / Q000494:pharmacology*; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D008099:Liver / Q000196:embryology* / Q000201:enzymology; D011239:Prednisolone / Q000494:pharmacology; D011247:Pregnancy; D011817:Rabbits
D002087:Butyrates; D020148:Butyric Acid; D003609:Dactinomycin; D003994:Bucladesine; D003513:Cycloheximide; D011239:Prednisolone; D000469:Alkaline Phosphatase