Monoclonal antibodies to gamma-interferon treated LAN-1 cells detect modulation of ganglioside GD2 exposure on human neuroblastoma cells.
A panel of 8 new Mabs have been produced against neuroblastoma cells (LAN-1) previously treated with IFN-gamma. All selected Mabs from 2 different fusions have been shown to detect epitopes on the GD2 ganglioside molecules highly expressed on all cells of neural crest origin including neuroblastoma, glioblastoma and melanoma. Our results imply that modulation of GD2 exposure on NB cells is dependent on culture conditions and moreover that IFN-gamma increases the surface expression of GD2 and thereby enhances their immunogenicity.
Gross
N
N
Department of Pediatrics, CHUV, Lausanne, Switzerland.
Beck
D
D
Portoukalian
J
J
Carrel
S
S
Favre
S
S
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis; D002460:Cell Line; D005732:Gangliosides / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D007371:Interferon-gamma / Q000494:pharmacology*; D009447:Neuroblastoma; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D005732:Gangliosides; D011994:Recombinant Proteins; C026226:ganglioside, GD3; D007371:Interferon-gamma