title: Nucleotide sequence of the Stp-1 gene coding for rat spermatid nuclear transition protein 1 (TP1): homology with protamine P1 and assignment of the mouse Stp-1 gene to chromosome 1.
abstract: Spermatid transition protein 1 (TP1) is a 54 amino acid (aa), highly basic chromosomal protein found in mammals during the brief period when histones are being replaced by protamines in the haploid phase of spermatogenesis. Using a cDNA clone as probe, we have isolated the gene (Stp-1) coding for rat TP1 from a population of recombinant bacteriophage lambda. The nucleotide (nt) sequence was established from a point 126 nt upstream from the mRNA cap site to a point about 30 nt downstream from the predicted site of polyadenylation. The gene contains a single intron separating the codon for aa 45 of the mature protein. Comparison of the nucleotide sequences for Stp-1 and the mouse gene coding for protamine P1 suggests a possible evolutionary relationship. Southern blot hybridization to genomic DNA isolated from a panel of mouse-hamster somatic cell hybrids unambiguously mapped Stp-1 to mouse chromosome 1.
authors.affiliation: Department of Chemistry, University of South Carolina, Columbia 29208.
authors.forename: M A
authors.initials: MA
authors.lastname: Heidaran
authors.forename: C A
authors.initials: CA
authors.lastname: Kozak
authors.forename: W S
authors.initials: WS
authors.lastname: Kistler
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D015139:Blotting, Southern; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D006224:Cricetinae; D004247:DNA / Q000302:isolation & purification; D015342:DNA Probes; D006822:Hybrid Cells; D051379:Mice; D008969:Molecular Sequence Data; D011479:Protamines / Q000235:genetics*; D051381:Rats; D012689:Sequence Homology, Nucleic Acid
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D015342:DNA Probes; D011479:Protamines; C046533:protamine P1; C053886:spermatid transition proteins; D004247:DNA