Assessment of canine intestinal permeability, using 51Cr-labeled ethylenediaminetetraacetate.
The 51Cr-labeled EDTA was validated as a suitable permeability probe in dogs for measurement of passive, unmediated diffusion across intestinal mucosa via intercellular pathways. The 51Cr-labeled EDTA was stable in aqueous solution and did not bind to biologic tissue and fluids. After incubation of 51Cr-labeled EDTA in isolated jejunal loops, analytic subcellular fractionation of jejunal mucosa on reorientating sucrose-density gradients was performed, and no association of 51Cr-labeled EDTA with particulate intracellular organelles was detected. Intravenously administered 51Cr-labeled EDTA was rapidly and completely excreted in urine. Intestinal permeability to 51Cr-labeled EDTA after oral administration was assessed in healthy dogs. The percentage of the administered dose of 51Cr-labeled EDTA excreted in the urine in 24 hours ranged from 2.3 to 17.6% (median, 13%).
Hall
E J
EJ
Department of Veterinary Pathology, University of Liverpool, United Kingdom.
Batt
R M
RM
Brown
A
A
D000818:Animals; D002860:Chromium Radioisotopes / Q000493:pharmacokinetics; D004285:Dogs; D004492:Edetic Acid / Q000008:administration & dosage / Q000493:pharmacokinetics*; D005260:Female; D007408:Intestinal Absorption; D007583:Jejunum / Q000032:analysis / Q000378:metabolism; D007678:Kidney Glomerulus / Q000378:metabolism; D008297:Male; D010539:Permeability
D002860:Chromium Radioisotopes; D004492:Edetic Acid