title: [The use of the multicopy plasmid pUC19 for assuring the constitutive expression of gene rplL in Escherichia coli].
abstract: A mutation in lac-operator region of pUC19 plasmid causing an increase in beta-galactosidase activity was observed. The plasmid was used as a vector to provide high level of expression of the cloned E. coli rplL gene.
authors.forename: S B
authors.initials: SB
authors.lastname: Zolotukhin
authors.forename: A N
authors.initials: AN
authors.lastname: Zhivolup
authors.forename: I V
authors.initials: IV
authors.lastname: Krupskaia
authors.forename: M I
authors.initials: MI
authors.lastname: Budmaska
authors.forename: E B
authors.initials: EB
authors.lastname: Paton
mesh_terms: D003001:Cloning, Molecular / Q000379:methods; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005822:Genetic Vectors; D007763:Lac Operon; D009154:Mutation; D010641:Phenotype; D010957:Plasmids / Q000235:genetics*; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D001616:beta-Galactosidase
vernacular_title: Ispol'zovanie mnogokopiĭnoĭ plazmidy pUC19 dlia obespecheniia konstitutivnoĭ ékspressii gena rplL Escherichia coli.