title: Perturbation of the idiotypic network. II. Transfer of suppression to neonatal mice.
abstract: The cellular mechanisms which are responsible for idiotype perturbation induced by repeated stimulation with either allogeneic mixed lymphocyte culture-generated syngeneic blasts or allogeneically stimulated syngeneic spleen cells were investigated as described in the preceding article. Using the splenic fragment culture system, the precursor frequencies of T and B cells for anti-phosphorylcholine (PC) antibodies and T15 idiotypic antibodies were determined in allogeneically challenged mice. Adoptive transfers of T cells to neonatal BALB/c mice induced suppression of the T15+ anti-PC responses. In addition, the effect of immunization with internal image-bearing anti-idiotopes on the level of anti-PC antibodies and T15 idiotype was determined. Results from this study demonstrated (i) a decrease in the precursor frequency in the PC-specific and idiotype-specific B cell repertoire; (ii) a decrease in the precursor frequency of T helper cells, which recognize idiotypes and anti-idiotypes; (iii) the possibility to transfer T15 suppression to neonatal mice; and (iv) the possibility to restore T15 dominance by anti-idiotypic antibody immunization. These data indicate that both the T and B compartments are involved in the maintenance of suppression induced by repeated exposure to alloantigen-sensitized syngeneic cells. Collectively these findings show that a nonspecific general suppression induced by allohyperimmunization can perturb the T15+ anti-PC response.
authors.affiliation: IDEC Pharmaceuticals Corporation, La Jolla, California 92037.
authors.forename: H C
authors.initials: HC
authors.lastname: Chang
authors.forename: S
authors.initials: S
authors.lastname: Müller
authors.forename: H
authors.initials: H
authors.lastname: Köhler
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000276:immunology; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology; D006412:Hematopoietic Stem Cells; D007108:Immune Tolerance; D007116:Immunization, Passive; D007130:Immunoglobulin Idiotypes / Q000032:analysis* / Q000276:immunology; D007519:Isoantigens / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D010767:Phosphorylcholine / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology
chemical_list: D007130:Immunoglobulin Idiotypes; D007519:Isoantigens; D010767:Phosphorylcholine