A rapid and direct method for the detection and quantification of interleukin-1 receptors using 96 well filtration plates.
A rapid method for the detection and quantification of interleukin-1 receptors in cultured cells has been developed. The receptor binding assay is carried out in sealed 96 well filtration plates. At the end of the incubation period the seal is removed and the cells are filtered under vacuum to separate free ligand from bound. After washing several times, individual wells are removed using a well punch and counted in a gamma counter. The method is rapid, accurate and capable of high sample throughput and should find wide application as a screen to evaluate IL-1-like drugs.
Horuk
R
R
Medical Products Division, E.I. Du Pont De Nemours & Co., Glenolden, PA 19036.
D002460:Cell Line; D002498:Centrifugation / Q000379:methods; D005374:Filtration / Q000295:instrumentation; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism*; D007700:Kinetics; D008567:Membranes, Artificial; D011869:Radioligand Assay / Q000295:instrumentation* / Q000379:methods; D011971:Receptors, Immunologic / Q000032:analysis*; D017472:Receptors, Interleukin-1; D015203:Reproducibility of Results
D007375:Interleukin-1; D008567:Membranes, Artificial; D011971:Receptors, Immunologic; D017472:Receptors, Interleukin-1