title: Human monoclonal antibody against a gag-coded protein of human immunodeficiency virus produced by a stable EBV-transformed cell clone.
abstract: An EBV-transformed lymphoblastoid B cell clone (A12) derived from peripheral blood lymphocytes of an HIV-1-infected individual is described. The immunoglobulin isotype produced by this clone was IgM, and Southern blot analysis of immunoglobulin gene rearrangement showed a monoclonal pattern. The A12 monoclonal antibody was specific for the p24 product of the HIV-1 gag gene. This clone is now in continuous culture for more than 8 months and no changes in its biologic properties have been observed.
authors.affiliation: Institute of Oncology, University of Padua, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Amadori
authors.forename: V
authors.initials: V
authors.lastname: Ciminale
authors.forename: M L
authors.initials: ML
authors.lastname: Calabro
authors.forename: L
authors.initials: L
authors.lastname: Tessarollo
authors.forename: E
authors.initials: E
authors.lastname: Francavilla
authors.forename: L
authors.initials: L
authors.lastname: Chieco-Bianchi
mesh_terms: D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D001402:B-Lymphocytes / Q000276:immunology; D002461:Cell Line, Transformed; D002999:Clone Cells; D015488:HIV Antigens / Q000276:immunology*; D016655:HIV Core Protein p24; D006679:HIV Seropositivity / Q000276:immunology; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D007075:Immunoglobulin M / Q000096:biosynthesis*; D012191:Retroviridae Proteins / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D015488:HIV Antigens; D016655:HIV Core Protein p24; D007075:Immunoglobulin M; D012191:Retroviridae Proteins