[Antigen presenting cells and T lymphocytes in the induction phase of contact hypersensitivity].
When a sensitizing substance that induces contact hypersensitivity, fluorescein isothiocyanate (FITC), was painted on abdominal skin of mice, FITC+ cells appeared in the inguinal lymph node after 24 hours. The FITC+ cell in the lymph node was relatively large in size, and it did not appear to be a T lymphocyte. When FITC was painted on either murine tail skin or skin pre-treated by tape stripping, the number of FITC+ cells in the inguinal lymph node was significantly less than that in the positive control. In the mesenteric lymph nodes, which have a different lymph flow from that of the skin regional lymph node, FITC+ cells did not increase in number, and the few FITC+ cells were not significantly different in number among above-mentioned experimental systems. In the inguinal lymph nodes on the 4th day after painting of picryl chloride (PCl) on the abdominal skin of mice, L3T4+ cells, which expressed an interleukin 2 receptor (IL-2R), increased in number. On the other hand, when PCl was painted on either tail skin or skin treated by tape stripping, L3T4+ IL-2R+ cells did not increase in the skin regional lymph nodes. The number of L3T4+ IL-2R+ cells in the mesenteric lymph nodes did not increase in any of the experimental systems mentioned above. These results suggest some relationship between antigen presenting cells and T lymphocytes, as well as one between the skin and the regional lymph nodes, in an induction phase of sensitization in contact hypersensitivity.
Morita
H
H
Yu
M
M
Miyazaki
T
T
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D003877:Dermatitis, Contact / Q000276:immunology* / Q000473:pathology; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D007114:Immunization; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology; D051379:Mice; D008809:Mice, Inbred C3H; D010853:Picryl Chloride; D013601:T-Lymphocytes / Q000276:immunology*; D013861:Thiocyanates
D005452:Fluoresceins; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate; D010853:Picryl Chloride