Effects of clarithromycin on cytochrome P-450. Comparison with other macrolides.
Repeated administration of clarithromycin (0.5 mmol.kg-1 p.o. daily for 5 days) to rats increased markedly the same cytochrome P-450 isoenzyme (P-450p) as that induced by troleandomycin. Clarithromycin, however, did not form cytochrome P-450 Fe(II)-metabolite complexes in vitro with microsomes from clarithromycin-treated rats or in vivo after repeated doses of clarithromycin. Nevertheless, clarithromycin formed cytochrome P-450 Fe(II)-metabolite complexes with microsomes from dexamethasone-treated rats in vitro, or after administration to dexamethasone-treated rats in vivo. Similar effects were observed with roxithromycin. In contrast, erythromycin and troleandomycin formed metabolic complexes when given alone, whereas josamycin, midecamycin and spiramycin did not form complexes, even in dexamethasone-treated rats. We conclude that clarithromycin and roxithromycin induce cytochrome P-450p, but do not form complexes with this isoenzyme, although they do form complexes with other glucocorticoid-inducible isoenzymes. We propose that macrolides may be classified into three groups, those forming complexes when given alone (e.g., erythromycin and troleandomycin), those forming complexes only in glucocorticoid-pretreated rats (clarithromycin and roxithromycin) and those not forming complexes (josamycin, midecamycin and spiramycin).
Tinel
M
M
Unité de Recherches de Physiopathologie Hépatique (Institut National de la Santé et de la Recherche Médicale U 24), Hôpital, Beaujon, Clichy, France.
Descatoire
V
V
Larrey
D
D
Loeper
J
J
Labbe
G
G
Letteron
P
P
Pessayre
D
D
D000818:Animals; D017291:Clarithromycin; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D003640:Dealkylation; D003907:Dexamethasone / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D004917:Erythromycin / Q000031:analogs & derivatives* / Q000494:pharmacology; D007501:Iron / Q000378:metabolism; D007527:Isoenzymes / Q000096:biosynthesis*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014217:Troleandomycin / Q000494:pharmacology
D007527:Isoenzymes; D004917:Erythromycin; D003907:Dexamethasone; D003577:Cytochrome P-450 Enzyme System; D014217:Troleandomycin; D007501:Iron; D017291:Clarithromycin