The effect of human C-reactive protein on the cell-attachment activity of fibronectin and laminin.
We have previously reported that purified human C-reactive protein (CRP) specifically binds to the cell-binding region of plasma fibronectin (Fn) in a Ca2+-dependent reaction that is saturable at a molar ratio of CRP/Fn of approximately 9. In this study, the binding of CRP to Fn was found to interfere with the cell-attachment promoting activity of Fn. The inhibition of cell attachment was dependent on the concentration of the CRP and involved the phosphorylcholine (PC) binding site of CRP since inhibition was prevented by allowing the CRP to react with either PC (or closely related monophosphate compounds) or a mAb specific for the PC-binding site of CRP. Binding of CRP to laminin was also Ca2+-dependent; however, this binding did not alter the cell-attachment promoting activity of laminin. CRP by itself does not mediate cell attachment. Since CRP is selectively deposited at sites of tissue damage along with plasma Fn and has the ability to bind to Fn and alter its cell-binding activity, CRP may modulate early events in tissue repair.
Tseng
J
J
Department of Microbiology, Ohio State University, Columbus 43210.
Mortensen
R F
RF
D000818:Animals; D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D002097:C-Reactive Protein / Q000378:metabolism / Q000502:physiology*; D002448:Cell Adhesion / Q000187:drug effects*; D005347:Fibroblasts / Q000378:metabolism / Q000502:physiology; D005353:Fibronectins / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D007797:Laminin / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D051381:Rats; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic / Q000032:analysis; D017474:Receptors, Laminin
D000911:Antibodies, Monoclonal; D005353:Fibronectins; D007797:Laminin; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic; D017474:Receptors, Laminin; D002097:C-Reactive Protein