Isolation and sequencing of cDNA clones encoding Drosophila chromosomal protein D1. A repeating motif in proteins which recognize at DNA.
Drosophila melanogaster D1 is a satellite DNA-associated protein which preferentially binds DNA sequences containing runs of AT base pairs. Clones encoding this polypeptide have been isolated from a lambda gt11 cDNA library by immunological screening with a D1 antiserum. The deduced sequence of the D1 polypeptide is 355 amino acids long and contains 10 copies of a repeating motif consisting of a glycine-arginine-proline (GRP) tripeptide located within a cluster of basic amino acids. Three copies of a similar motif have previously been observed in a mammalian satellite DNA-binding protein, high mobility group protein I (Lund, T., Dahl, K. H., Mork, E., Holtlund, J., and Laland, S. G. (1987) Biochem. Biophys. Res. Commun. 146, 725-730), suggesting that this motif may be a general feature of proteins which bind AT-rich satellite DNA and perhaps other AT-containing DNA as well.
Ashley
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Department of Biochemistry, University of Georgia, Athens 30602.
Pendleton
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Jennings
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Saxena
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A
Glover
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D000595:Amino Acid Sequence; D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D001665:Binding Sites; D000071877:Checkpoint Kinase 1; D003488:Cyanogen Bromide; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D004262:DNA Restriction Enzymes; D004268:DNA-Binding Proteins / Q000235:genetics*; D015246:Deoxyribonuclease EcoRI; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D005033:Ethylmaleimide; D007118:Immunoassay; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010766:Phosphorylation; D012091:Repetitive Sequences, Nucleic Acid
D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D009687:Nuclear Proteins; D004247:DNA; D000071877:Checkpoint Kinase 1; C000605877:grp protein, Drosophila; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; D005033:Ethylmaleimide; D003488:Cyanogen Bromide