Prostaglandin E2 selectively increases interferon gamma receptor expression on human CD8+ lymphocytes.
We previously reported that prostaglandin E2 (PGE2) at a physiologic concentration (10(-8) M) and interferon gamma (IFN gamma), acting sequentially, were required for the differentiation of suppressor cells in mitogen-stimulated cultures. The present study was designed to test whether PGE2 might mediate IFN gamma-dependent effects on CD8+ cells by altering the number and/or affinity of their IFN gamma receptors. CD8+ and CD4+ cells when cultured for 18 h expressed comparable numbers of IFN gamma receptors of a single high affinity. Incubation with 10(-8) M PGE2 for 18 h, however, increased the number of IFN gamma receptors on CD8+ cells without affecting the binding affinity. Similar effects were not observed with CD4+ cells, nor when CD8+ cells were cultured in 10(-8) M PGD2. Concentrations of PGE2, which were ineffective in the induction of IFN gamma-dependent suppressor cell differentiation, also did not affect IFN gamma receptor expression on CD8+ cells. This observation of a specific stimulatory effect of PGE2 on the display of IFN gamma receptors of CD8+ cells suggests a novel mechanism for eicosanoid function through tissue-specific regulation of hormone receptors.
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Howard Hughes Medical Institute Laboratory, Baylor College of Medicine, Houston, Texas 77030.
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D000945:Antigens, Differentiation, T-Lymphocyte; D016827:CD8 Antigens; D015232:Dinoprostone / Q000494:pharmacology*; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism*; D010641:Phenotype; D011971:Receptors, Immunologic / Q000096:biosynthesis* / Q000187:drug effects; D017471:Receptors, Interferon; D050378:T-Lymphocytes, Regulatory / Q000145:classification / Q000378:metabolism*
D000945:Antigens, Differentiation, T-Lymphocyte; D016827:CD8 Antigens; D011971:Receptors, Immunologic; D017471:Receptors, Interferon; D007371:Interferon-gamma; D015232:Dinoprostone