Ischemia-induced changes in myocardial paramagnetic metabolites: implications for intracellular oxy-radical generation.
Evidence to identify the cellular sources of oxy-radical generation in myocardium has been of an indirect nature. We have used low-temperature ESR spectroscopy to identify and characterize ischemia-induced changes in myocardial paramagnetic metabolites. Iron-sulfur proteins associated with the NADH or succinate dehydrogenases of the mitochondrial electron-transport chain were progressively reduced with the onset and development of ischemia. This study provides direct evidence for ischemia-induced changes in an intracellular source of superoxide radical generation that may contribute to oxy-radical production during reperfusion.
Baker
J E
JE
Department of Cardiothoracic Surgery, Medical College of Wisconsin, Milwaukee 53226.
Kalyanaraman
B
B
D000818:Animals; D003327:Coronary Disease / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D005609:Free Radicals; D066298:In Vitro Techniques; D008954:Models, Biological; D009206:Myocardium / Q000378:metabolism*; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains
D005609:Free Radicals