title: Quantitative rather than qualitative differences between monocytes with respect to IgE Fc receptor expression as studied by flow cytofluorometry.
abstract: In this study we have used aggregated human IgE, conjugated with fluoroisothiocyanate, as a ligand in flow cytofluorometry to detect the low-affinity Fc receptor for IgE on human leukocytes. The percentage of IgE-binding monocytes increased up to 44%, in a dose-related manner, as the concentration of aggregated IgE in the fluid phase increased to the highest concentration (300 micrograms/ml) used in this study. No such dose-related increase was shown for granulocytes (1.8% IgE-binding cells) or lymphocytes (2.1% IgE-binding cells). The IgE-binding was inhibited by IgE but not by IgG, whereas the opposite effect was obtained for IgG binding. We could not verify a distinct IgE-binding subpopulation of monocytes in peripheral blood. The results rather indicate that most monocytes bind IgE to a variable degree depending on the concentration of the ligand in the fluid phase.
authors.affiliation: Department of Clinical Immunology, Karolinska Hospital, Stockholm, Sweden.
authors.forename: J
authors.initials: J
authors.lastname: Hed
authors.forename: G
authors.initials: G
authors.lastname: Halldén
authors.forename: S G
authors.initials: SG
authors.lastname: Johansson
authors.forename: P
authors.initials: P
authors.lastname: Larsson
mesh_terms: D000944:Antigens, Differentiation, B-Lymphocyte / Q000096:biosynthesis*; D002452:Cell Count; D004306:Dose-Response Relationship, Immunologic; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism*; D007074:Immunoglobulin G / Q000378:metabolism; D066298:In Vitro Techniques; D009000:Monocytes / Q000166:cytology / Q000378:metabolism*; D011961:Receptors, Fc / Q000096:biosynthesis*; D017455:Receptors, IgE; D013861:Thiocyanates
chemical_list: D000944:Antigens, Differentiation, B-Lymphocyte; D005452:Fluoresceins; D007074:Immunoglobulin G; D011961:Receptors, Fc; D017455:Receptors, IgE; D013861:Thiocyanates; D007073:Immunoglobulin E; D016650:Fluorescein-5-isothiocyanate