title: Molecular structure and expression of the murine lymphocyte low-affinity receptor for IgE (Fc epsilon RII).
abstract: The cDNA encoding the murine low-affinity receptor for IgE (Fc epsilon RII) has been isolated from a cDNA library prepared from B cells activated with lipopolysaccharide and interleukin 4. It encodes a 37-kDa protein of 331 amino acids with two potential N-linked glycosylation sites. Analogous to its human counterpart, there is no signal sequence and the putative transmembrane region is close to the amino terminus, indicating an inverse membrane orientation with the carboxyl terminus at the cell exterior. The predicted murine Fc epsilon RII amino acid sequence demonstrates a 57% identity with its human counterpart. The murine sequence has an additional internal repeat motif of 21 amino acids giving four repeats as compared to three in the human sequence. Furthermore, the murine Fc epsilon RII is truncated at the carboxyl terminus and the Arg-Gly-Asp sequence, a common recognition site of integrin receptors, which is found in the reverse configuration in human Fc epsilon RII, is missing. B cells activated with interleukin 4 and lipopolysaccharide have an increased amount of Fc epsilon RII mRNA as compared with resting or lipopolysaccharide-stimulated B cells. Con A-activated normal T cells, the TH-2 cell line D10, as well as the macrophage cell line J774 have no detectable Fc epsilon RII mRNA. Expression analysis using transiently transfected COS cells revealed that recombinant murine Fc epsilon RII binds anti-Fc epsilon RII as well as mouse and rat IgE but does not bind human IgE or mouse IgG. Fc epsilon RII expressed in COS cells has a molecular mass of 45 kDa whereas the Fc epsilon RII from B-cell lines is a 49-kDa protein.
authors.affiliation: Biotechnology Department, Ciba-Giegy Ltd., Basel, Switzerland.
authors.forename: B
authors.initials: B
authors.lastname: Bettler
authors.forename: H
authors.initials: H
authors.lastname: Hofstetter
authors.forename: M
authors.initials: M
authors.lastname: Rao
authors.forename: W M
authors.initials: WM
authors.lastname: Yokoyama
authors.forename: F
authors.initials: F
authors.lastname: Kilchherr
authors.forename: D H
authors.initials: DH
authors.lastname: Conrad
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte / Q000235:genetics*; D001402:B-Lymphocytes / Q000276:immunology*; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D015723:Gene Library; D005796:Genes; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D011961:Receptors, Fc / Q000235:genetics*; D017455:Receptors, IgE; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
chemical_list: D000944:Antigens, Differentiation, B-Lymphocyte; D011961:Receptors, Fc; D017455:Receptors, IgE; D007073:Immunoglobulin E; D004247:DNA