Isolation of a gene that down-regulates nitrate assimilation and influences another regulatory gene in the same system.
Glutamine is the preferred source of nitrogen of Neurospora crassa. In its presence and that of the gene product of MS5 (nmr-1), the fungus represses the assimilation of less preferred forms of nitrogen, such as nitrate. In the absence of glutamine and the presence of the product of gene nit-2, less preferred forms of nitrogen are assimilated as long as a specific pathway for their assimilation is induced. We report here the isolation, from a cosmid bank, of a gene that complements MS5 and can also complement nit-2. We speculate that this result suggests an interaction between the MS5 and nit-2 gene products and that this is important in the regulation of nitrate assimilation.
Sorger
G J
GJ
Department of Biology, McMaster University, Hamilton, Ontario, Canada.
Brown
D
D
Farzannejad
M
M
Guerra
A
A
Jonathan
M
M
Knight
S
S
Sharda
R
R
D003360:Cosmids; D005786:Gene Expression Regulation; D005800:Genes, Fungal; D005809:Genes, Regulator; D005816:Genetic Complementation Test; D005973:Glutamine / Q000378:metabolism; D009154:Mutation; D009492:Neurospora crassa / Q000235:genetics / Q000378:metabolism; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000235:genetics / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism*; D010957:Plasmids; D015183:Restriction Mapping; D014170:Transformation, Genetic
D009566:Nitrates; D005973:Glutamine; D009565:Nitrate Reductases; D050901:Nitrate Reductase