title: Common structural changes accompany the functional inactivation of HPr by seryl phosphorylation or by serine to aspartate substitution.
abstract: Although many proteins are known to be regulated via reversible phosphorylation, little is known about the mechanism by which the covalent modification of seryl, threonyl, or tyrosyl residues alters the activities of the target systems. To address this question, modified versions of Bacillus subtilus HPr, a protein component of the bacterial phosphotransferase system, have been studied by 1H NMR spectroscopy. Phosphorylation at Ser46 or a Ser to Asp substitution at this position inactivates HPr [Reizer, J., Sutrina, S. L., Saier, M. H., Stewart, G. C., Peterkofsky, A., & Reddy, P. (1989) EMBO J. 8, 2111-2120]. Two-dimensional spectra of these two modified proteins display nearly identical proton chemical shifts that differ significantly from those observed in the spectra of the unphosphorylated, wild-type protein and of functionally active HPr mutants. The results demonstrate that the functional inactivation of HPr brought about by the serine to aspartate mutation is accompanied by the same structural changes that occur when HPr is phosphorylated at Ser46.
authors.affiliation: Department of Biochemistry, University of Washington, Seattle 98195.
authors.forename: M
authors.initials: M
authors.lastname: Wittekind
authors.forename: J
authors.initials: J
authors.lastname: Reizer
authors.forename: J
authors.initials: J
authors.lastname: Deutscher
authors.forename: M H
authors.initials: MH
authors.lastname: Saier
authors.forename: R E
authors.initials: RE
authors.lastname: Klevit
mesh_terms: D001224:Aspartic Acid / Q000378:metabolism; D001426:Bacterial Proteins; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D015394:Molecular Structure; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000378:metabolism*; D010766:Phosphorylation; D012694:Serine / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D001224:Aspartic Acid; D012694:Serine; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C030182:phosphocarrier protein HPr