A new commercial method for the enzymatic determination of creatinine in serum and urine evaluated: comparison with a kinetic Jaffé method and isotope dilution-mass spectrometry.
We evaluated a new, simple, enzymatic kinetic method from Wako Chemicals GmbH in comparison with a kinetic Jaffé method by using isotope dilution-mass spectrometry (ID-MS) as a reference method. An ID-MS-calibrated serum standard was used. Both the enzymatic and the Jaffé method correlated well with ID-MS, except for sera with high concentrations of bilirubin. Ethyl acetoacetate, acetone, and glucose in serum interfered somewhat with the Jaffé method but not with the enzymatic method. We conclude that the present enzymatic method has merit as compared with a Jaffé method for routine work, but is more expensive.
Lindbäck
B
B
Department of Clinical Chemistry, Huddinge University Hospital, Sweden.
Bergman
A
A
D000581:Amidohydrolases; D003404:Creatinine / Q000032:analysis* / Q000097:blood / Q000652:urine; D005069:Evaluation Studies as Topic; D006801:Humans; D007677:Kidney Function Tests; D007700:Kinetics; D013058:Mass Spectrometry; D010089:Oxidoreductases, N-Demethylating; D009195:Peroxidase; D011865:Radioisotope Dilution Technique; D011933:Reagent Kits, Diagnostic; D050879:Sarcosine Oxidase
D011933:Reagent Kits, Diagnostic; D003404:Creatinine; D009195:Peroxidase; D010089:Oxidoreductases, N-Demethylating; D050879:Sarcosine Oxidase; D000581:Amidohydrolases; C019946:creatininase