[Comparison of methods for the demonstration of Treponema-specific IgM].
In this research 113 sera have been analysed with three methods for IgM treponema-specific determination: IgM-SPHA, IgM-EIA and 19S IgM FTA-ABS. Among these sera, 33 samples related to non-treated patients at different stages of infection, and 80 samples to treated patients. The results point out a light sensibility of the IgM-SPHA in primary lues. The IgM-EIA test has performed a good specificity, but displayed a sensibility lower to the 19S IgM FTA-ABS, which proved the best of tests. About the cases of treated lues at different stages, in 23 samples with VDRL negative has been found no positivity at three tests used, while in 49 samples with VDRL positive 8 are resulted positive at 19S IgM FTA-ABS.
Panuccio
A
A
Istituto di Microbiologia dell'Università di Milano.
Borroni
G
G
Gelosa
L
L
D000907:Antibodies, Bacterial / Q000097:blood*; D002308:Cardiolipins; D002784:Cholesterol; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D005779:Immunodiffusion; D007075:Immunoglobulin M / Q000032:analysis*; D010713:Phosphatidylcholines; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D013588:Syphilis Serodiagnosis / Q000379:methods*; D013592:Syphilis, Latent / Q000175:diagnosis; D014210:Treponema pallidum / Q000276:immunology*
D000907:Antibodies, Bacterial; D002308:Cardiolipins; D007075:Immunoglobulin M; D010713:Phosphatidylcholines; D011933:Reagent Kits, Diagnostic; C054448:VDRL antigen; D002784:Cholesterol
Metodologie a confronto per la ricerca di IgM Treponema-specifiche.