Comparison of muscarinic cholinergic and alpha adrenergic receptors in canine ileum, colon, intestinal urinary reservoirs and bladder.
The muscarinic cholinergic (MCh) and alpha 2 adrenergic receptor densities in canine ileum, colon, ileal and colonic urinary reservoirs and bladder were determined using radioligand receptor binding methods in order to provide a rational basis for pharmacologic management of urinary incontinence following bladder replacement with intestinal segments. Muscarinic cholinergic and alpha 2 adrenergic receptor binding sites were studied in these tissues using saturation experiments with 3H-NMS and 3H-rauwolscine, respectively. The mean equilibrium dissociation constants for 3H-NMS binding (0.13 to 0.17 nM) in these tissues were similar (p greater than 0.05) indicating homogeneity of muscarinic cholinergic binding sites. The mean equilibrium dissociation constants for 3H-rauwolscine binding (1.27 to 1.98 nM) in these tissues were also similar (p greater than 0.05). A substantial density of MCh (1.06 to 1.22 fmol/mg. wet wt.) and alpha 2 adrenergic (0.47 to 1.11 fmol/mg. wet wt.) binding sites was identified in the intestinal tissues assayed. The density of ileal and colonic MCh and alpha 2 adrenergic binding sites was not altered following construction of urinary intestinal reservoirs. The presence of a substantial density of MCh and alpha 2 adrenergic binding sites in the intestinal tissues suggests that MCh and alpha 2 adrenergic analogs may be utilized for the management of urinary incontinence following bladder replacement with intestinal urinary reservoirs.
Lepor
H
H
Department of Surgery (Urology), Washington University School of Medicine, Jewish Hospital, St. Louis, MO 63110.
Mohler
J
J
Baumann
M
M
Shapiro
E
E
D000818:Animals; D003106:Colon / Q000294:innervation; D004285:Dogs / Q000033:anatomy & histology*; D007082:Ileum / Q000294:innervation; D007422:Intestines / Q000294:innervation* / Q000601:surgery; D011942:Receptors, Adrenergic, alpha / Q000032:analysis; D011976:Receptors, Muscarinic / Q000032:analysis; D017981:Receptors, Neurotransmitter / Q000032:analysis*; D001743:Urinary Bladder / Q000294:innervation*; D014547:Urinary Diversion; D014549:Urinary Incontinence / Q000628:therapy
D011942:Receptors, Adrenergic, alpha; D011976:Receptors, Muscarinic; D017981:Receptors, Neurotransmitter