Glial fibrillary acidic protein (GFAP) from goldfish: its localisation in visual pathway.
An intermediate filament fraction, isolated from goldfish brain, contains a prominent protein having a molecular weight of 51 kDa. In normal goldfish visual pathway, this protein is present in tectum and tract, but not in optic nerve. A polyclonal antibody raised to this protein clearly labels ependymal glial profiles in tectum and parallel processes in the tract, whereas optic nerve is unlabelled; Müller fibres in the retina are also labelled. A similar, but less prominent, pattern of staining is observed with antibodies, raised elsewhere, against glial fibrillary acidic protein from human and porcine. These results suggest that the 51 kDa protein is a GFAP, demonstrate the heterogeneity of astrocytes in goldfish visual pathway, and are consistent with the idea that GFAP is well conserved in vertebrate phylogeny.
Nona
S N
SN
Department of Optometry and Vision Sciences, University of Manchester Institute of Science & Technology, England.
Shehab
S A
SA
Stafford
C A
CA
Cronly-Dillon
J R
JR
D000818:Animals; D003530:Cyprinidae / Q000378:metabolism*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism*; D006054:Goldfish / Q000378:metabolism*; D007150:Immunohistochemistry; D012160:Retina / Q000166:cytology / Q000378:metabolism*; D013477:Superior Colliculi / Q000378:metabolism*; D014795:Visual Pathways / Q000378:metabolism*
D005904:Glial Fibrillary Acidic Protein