Identification of single-strand initiation signals in the terC region of the Escherichia coli chromosome.
On the basis of clear-plaque formation, we detected initiation signals in the terC region of the Escherichia coli chromosome. At least two single-strand initiation signals were identified from the terC region. The nucleotide sequences of these two signals were determined. Sequence homologies, variations of the consensus of n' protein recognition sites, 5'-GAAGCGG-3', were found within these signals. A novel conserved sequence was also found within these signals. Their initiation activities were measured both by the infection growth assay and by the ability to convert the single-stranded DNA to the duplex replicative form DNA in vivo.
Hiasa
H
H
Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University, Japan.
Sakai
H
H
Komano
T
T
D010584:Bacteriophage phi X 174 / Q000235:genetics; D001483:Base Sequence; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004262:DNA Restriction Enzymes