title: Beta-endorphin in the brainstem and the cerebellum of the human infant: regional levels' profile assessed with immunoaffinity chromatography and solid phase radioimmunoassay.
abstract: The regional levels' profile of human beta-endorphin (beta h-EP) was studied in the brainstem and the cerebellum of 16 infant victims of "Sudden Infant Death Syndrome" and other death causes. An immunoaffinity chromatography procedure based on a monoclonal antibody directed specifically against the N-terminus of beta-EP was used to extract this peptide from the tissue samples. Beta-EP was then assessed quantitatively by means of a very sensitive solid phase radioimmunoassay (using a polyclonal antibody specific for the C-terminus of beta-EP) developed especially for the study presented here.
authors.affiliation: Institute of Forensic Medicine, University of Zurich, Switzerland.
authors.forename: A
authors.initials: A
authors.lastname: Pasi
authors.forename: P
authors.initials: P
authors.lastname: Kulling
authors.forename: D
authors.initials: D
authors.lastname: Voellmy
authors.forename: C
authors.initials: C
authors.lastname: Gramsch
authors.forename: P
authors.initials: P
authors.lastname: Mehraein
authors.forename: M
authors.initials: M
authors.lastname: Häni
authors.forename: F S
authors.initials: FS
authors.lastname: Messiha
mesh_terms: D001933:Brain Stem / Q000473:pathology*; D002531:Cerebellum / Q000473:pathology*; D002846:Chromatography, Affinity; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008526:Medulla Oblongata / Q000473:pathology; D008636:Mesencephalon / Q000473:pathology; D011149:Pons / Q000473:pathology; D011863:Radioimmunoassay; D013398:Sudden Infant Death / Q000473:pathology*; D001615:beta-Endorphin / Q000378:metabolism*
chemical_list: D001615:beta-Endorphin