A new bronchial asthma model using calcium ionophore A23187 in guinea pigs.
We attempted to develop a nonimmunologically induced asthma model using the calcium ionophore A23187. Inhalation of A23187 (0.001-0.005%) for 5 min in male Hartley guinea pigs caused a marked bronchoconstriction in a dose-dependent manner with negligible effect on systemic blood pressure. The A23187-induced bronchoconstriction was strongly inhibited by chlorpheniramine and FPL-55712. These results indicate that an asthma-like bronchoconstriction was induced by inhalation of A23187 in guinea pigs, and the main chemical mediators involved in this response would be histamine and peptidoleukotrienes.
Misawa
M
M
Department of Applied Pharmacology, School of Pharmacy, Hoshi University, Tokyo, Japan.
Kawamura
T
T
Takenouchi
K
K
D000280:Administration, Inhalation; D000818:Animals; D001249:Asthma / Q000139:chemically induced*; D000001:Calcimycin / Q000008:administration & dosage / Q000494:pharmacology*; D002744:Chlorpheniramine / Q000008:administration & dosage / Q000494:pharmacology; D002867:Chromones / Q000494:pharmacology; D004195:Disease Models, Animal; D006168:Guinea Pigs; D007275:Injections, Intravenous; D008297:Male; D013189:SRS-A / Q000037:antagonists & inhibitors
D002867:Chromones; D013189:SRS-A; D000001:Calcimycin; D002744:Chlorpheniramine; C006312:FPL 55712