Inward rectifier produced by Xenopus oocytes injected with mRNA extracted from carp olfactory epithelium.
Ionic channels encoded by mRNA extracted from carp olfactory epithelium were investigated by injection into Xenopus laevis oocytes. The oocytes expressed an inward rectifier K+ -channel, as detected under two-electrode voltage clamp conditions. The results were as follows. An inactivating inward current appeared on hyperpolarization and increased with increasing extracellular K+ concentrations. The 0 current potentials plotted as a function of log [K+]0 in the range between 2 to 20 mMK+ fell on a straight line, with a slope of 58 mV per tenfold change in K+ concentration, indicating that the current carrier is K+. Chord conductances reached saturation levels on extreme hyperpolarization. The chord conductances at the saturation levels were 35.7, 22.5, and 13.4 muSec in 20, 10, and 5 mM extracellular K+, respectively. Extracellular application of 0.1 mM Cs+ or 0.1 mM Ba2+ blocked the inward current in 2 mM K+, whereas 1 microM TTX or 0.3 mM Cd2+ did not affect the inward current. Inactivation of the inward currents, which became clear on extreme hyperpolarization, was suppressed with decreasing extracellular Na+ concentration. The present results suggest that carp olfactory epithelium is rich in the inward rectifier and is an excellent source of mRNA for cloning cDNA coding the inward rectifier.
Yoshii
K
K
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Kurihara
K
K
D000818:Animals; D002347:Carps; D004553:Electric Conductivity; D004847:Epithelial Cells; D005260:Female; D009830:Olfactory Bulb / Q000166:cytology*; D009865:Oocytes / Q000502:physiology*; D015221:Potassium Channels / Q000502:physiology; D012333:RNA, Messenger / Q000502:physiology; D015222:Sodium Channels / Q000502:physiology; D013779:Tetrodotoxin / Q000494:pharmacology; D014982:Xenopus laevis
D015221:Potassium Channels; D012333:RNA, Messenger; D015222:Sodium Channels; D013779:Tetrodotoxin