[In vitro activation of immune cells by a mycolic acid-containing glycolipid, trehalose-2,3,6'-trimycolate, derived from Gordona aurantiaca].
A mycolic acid-containing glycolipid, trehalose-2,3,6'-trimycolate (GaGM), derived from Gordona aurantiaca, an acid-fast bacteria closely related taxonomically to Mycobacterium, was investigated for its immune adjuvant activity in vitro. The liposomes containing GaGM showed strong mitogenic effects on murine spleen cells at the doses used (25-100 micrograms/ml), but not on T-cell-depleted spleen cells or macrophage-depleted spleen cells. These results suggest that the mitogenic property of liposomes containing GaGM differs from that of such as lipopolysaccharide, a B-cell mitogen and that its mitogenic effects depend on the presence of macrophages. In addition, liposomes containing GaGM augmented the mixed lymphocyte reaction (MLR) and in vitro induction of cytotoxic T-lymphocytes (CTLs) against allogeneic tumor cells. These results suggest that liposomes containing GaGM have immune adjuvant properties in vitro and the adjuvant activity may be related to such cytokines as interleukin-1 and -2.
Ohtsubo
Y
Y
Furukawa
M
M
Sugimoto
N
N
Ikutoh
M
M
Katoh
Y
Y
Yano
I
I
Shinka
S
S
Dohi
Y
Y
D000276:Adjuvants, Immunologic; D000818:Animals; D002478:Cells, Cultured; D006017:Glycolipids / Q000302:isolation & purification / Q000494:pharmacology*; D008081:Liposomes / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects*; D007959:Lymphocyte Culture Test, Mixed; D008264:Macrophages / Q000276:immunology; D008297:Male; D051379:Mice; D009161:Mycobacterium / Q000032:analysis*; D013154:Spleen / Q000166:cytology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000276:Adjuvants, Immunologic; D006017:Glycolipids; D008081:Liposomes; C060019:trehalose 2,3,6'-trimycolate