title: Monocyte-lymphocyte discrimination in a new microtitre-based adhesion assay.
abstract: A new, rapid and reproducible method is described for the quantification of in vitro monocyte adherence in microtitre 96-well ELISA plates. The assay is based on the measurement of the myeloperoxidase activity in monocytes that have adhered to either plastic or bovine/porcine aortic endothelial cells. The assay shows a linear response to monocyte concentrations over the range 5 x 10(4)-5 x 10(5) cells/well. The method provides good discrimination between monocyte and and lymphocytes and thus allows a study of mononuclear cell populations to be made. The assay works equally well with polymorphonuclear cells. The coefficients of variation are: intra-assay, 7%; interassay, 13%.
authors.affiliation: Department of Medicine, King's College Hospital, London, U.K.
authors.forename: P M
authors.initials: PM
authors.lastname: Bath
authors.forename: R F
authors.initials: RF
authors.lastname: Booth
authors.forename: D G
authors.initials: DG
authors.lastname: Hassall
mesh_terms: D002448:Cell Adhesion; D004730:Endothelium, Vascular / Q000166:cytology; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000494:pharmacology; D008077:Lipoproteins, LDL / Q000494:pharmacology; D008214:Lymphocytes / Q000166:cytology* / Q000201:enzymology; D009000:Monocytes / Q000166:cytology* / Q000201:enzymology; D009195:Peroxidase / Q000032:analysis*
chemical_list: D007375:Interleukin-1; D008077:Lipoproteins, LDL; D009195:Peroxidase