Sequencing of cloned DNA using bacteriophage lambda gt11 templates.
A procedure for isolating and directly sequencing recombinant bacteriophage lambda gt11 DNA templates is described. Approximately 250-300 bases of sequence can be obtained directly from the lambda gt11 template, eliminating the need for subcloning prior to dideoxynucleotide sequencing of clones.
Steffens
D L
DL
Div. of Molecular and Cellular Cardiovascular Biochemistry, Washington University School of Medicine, St. Louis, MO 63110.
Gross
R W
RW
D010582:Bacteriophage lambda / Q000235:genetics*; D001483:Base Sequence; D001709:Biotechnology; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics*
D004279:DNA, Viral