High incidence of coamplification of hst-1 and int-2 genes in human esophageal carcinomas.
We analyzed the alteration of the hst-1 and int-2 genes in 36 cases of esophageal squamous cell carcinoma, 42 cases of gastric adenocarcinoma, and 52 cases of colorectal adenocarcinoma. Coamplification of the hst-1 and int-2 genes was observed in 19 of 36 esophageal carcinomas (52%), 16 of 34 primary tumor tissues (47%), and 10 of 10 metastatic tumors (100%). The degree of amplification ranged from 4- to 8-fold. The incidence of hst-1 and int-2 gene coamplification was significantly higher in male patients than that in female patients (P less than 0.05). The coamplification of the hst-1 and int-2 genes had a tendency to correlate with clinical stage. The progesterone receptor gene, which is mapped to chromosome 11 at band q21-23, was not amplified in these esophageal carcinomas. Coamplification of the hst-1 and int-2 gene does not seem to imply increased numbers of chromosome 11, and the hst-1 and int-2 genes appear to be in same amplification unit on chromosome 11 at band q13. No coamplification of the hst-1 and int-2 genes was detected in gastric carcinomas and colorectal carcinomas. These results suggest that amplification of chromosomal locus of the hst-1 and int-2 genes might participate in carcinogenesis, in progression, and particularly in metastasis of esophageal carcinomas.
Tsuda
T
T
Department of Pathology, Hiroshima University School of Medicine, Japan.
Tahara
E
E
Kajiyama
G
G
Sakamoto
H
H
Terada
M
M
Sugimura
T
T
D015139:Blotting, Southern; D002277:Carcinoma / Q000235:genetics*; D002880:Chromosomes, Human, Pair 11; D015342:DNA Probes; D004938:Esophageal Neoplasms / Q000235:genetics*; D051518:Fibroblast Growth Factor 3; D051519:Fibroblast Growth Factor 4; D005346:Fibroblast Growth Factors; D005784:Gene Amplification; D006133:Growth Substances / Q000235:genetics*; D006493:Heparin; D006801:Humans; D009362:Neoplasm Metastasis; D015513:Oncogene Proteins / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D011980:Receptors, Progesterone / Q000235:genetics
D015342:DNA Probes; C496444:FGF3 protein, human; C496445:FGF4 protein, human; D051518:Fibroblast Growth Factor 3; D051519:Fibroblast Growth Factor 4; D006133:Growth Substances; D015513:Oncogene Proteins; D011518:Proto-Oncogene Proteins; D011980:Receptors, Progesterone; D005346:Fibroblast Growth Factors; D006493:Heparin