Phenotypic analysis of four human medulloblastoma cell lines and transplantable xenografts.
An extensive panel of monoclonal antibodies (MAb) and monospecific antisera reactive against neuroectodermal-, neuronal-, glial-, and lymphoid-associated antigens, extracellular matrix, HLA, and cell-surface receptors was used to characterize the phenotype of four continuous, karyotypically distinct medulloblastoma cell lines and transplantable xenografts. All four cell lines demonstrated significant reactivity with anti-neuroectodermal-associated MAb. No apparent pattern of reactivity with anti-lymphoid MAb was seen; notably, there was a uniform absence of detectable Thy-1. Review of the complete antibody reactivity profile revealed a dichotomy between lines TE-671 and Daoy and lines D283 Med and D341 Med, which have been previously shown to express neurofilament protein in culture and xenografts, and to exhibit neuroblastic morphological features in biopsy and xenograft tissue sections. TE-671 and Daoy reacted with the MAb directed against tenascin, epidermal growth factor (EGF) receptor, HLA-A,B epitopes, beta 2-microglobulin and 5/8 of the glioma-associated antigens, but did not react with the anti-neurofilament protein (NFP) MAb. D283 Med and D341 Med expressed NFP but did not react with MAb against tenascin, EGF receptor, HLA-A,B epitopes, beta 2-microglobulin or 6/8 and 7/8 (respectively) of the glioma-associated antigens. The observed phenotypic differences provide a conceptual framework for investigating basic differences in the biological behavior of medulloblastoma. Moreover, the subdivisions can be evaluated for prospective value in tissue diagnosis, cerebrospinal fluid cytology and antibody-mediated imaging and therapy.
He
X M
XM
Department of Pathology, Duke University Medical Center, Durham, NC 27710.
Skapek
S X
SX
Wikstrand
C J
CJ
Friedman
H S
HS
Trojanowski
J Q
JQ
Kemshead
J T
JT
Coakham
H B
HB
Bigner
S H
SH
Bigner
D D
DD
D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D004475:Ectoderm / Q000276:immunology; D005109:Extracellular Matrix / Q000276:immunology; D005260:Female; D006680:HLA Antigens / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007158:Immunologic Techniques; D008221:Lymphoid Tissue / Q000276:immunology; D008297:Male; D008527:Medulloblastoma / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D009368:Neoplasm Transplantation; D009457:Neuroglia / Q000276:immunology; D009474:Neurons / Q000276:immunology; D010641:Phenotype; D051381:Rats; D011923:Rats, Nude; D011956:Receptors, Cell Surface / Q000276:immunology; D014183:Transplantation, Heterologous
D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D007106:Immune Sera; D011956:Receptors, Cell Surface