Maize pyruvate decarboxylase mRNA is induced anaerobically.
A cDNA was identified using an oligonucleotide designed by comparing the sequences of bacterial and yeast pyruvate decarboxylase. The sequence of the cDNA identified by the oligonucleotide contained an open reading frame that encoded a protein of 65 kDa that was similar in sequence to bacterial and yeast pyruvate decarboxylase. This protein was selectively precipitated by an antiserum specific for maize PDC. Northern-blot analysis shows that PDC mRNA is anaerobically induced. Southern-blot analysis of maize genomic DNA indicated that the maize PDC gene has a single or low copy number.
Kelley
P M
PM
School of Biological Sciences, University of Nebraska, Lincoln 68588-0118.
D000595:Amino Acid Sequence; D000693:Anaerobiosis; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D004271:DNA, Fungal / Q000235:genetics; D004790:Enzyme Induction; D008969:Molecular Sequence Data; D010944:Plants / Q000201:enzymology / Q000235:genetics*; D011767:Pyruvate Decarboxylase / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D003313:Zea mays / Q000201:enzymology / Q000235:genetics
D015342:DNA Probes; D004271:DNA, Fungal; D012333:RNA, Messenger; D004247:DNA; D011767:Pyruvate Decarboxylase