denV gene of bacteriophage T4 restores DNA excision repair to mei-9 and mus201 mutants of Drosophila melanogaster.
The denV gene of bacteriophage T4 was fused to a Drosophila hsp70 (70-kDa heat shock protein) promoter and introduced into the germ line of Drosophila by P-element-mediated transformation. The protein product of that gene (endonuclease V) was detected in extracts of heat-shocked transformants with both enzymological and immunoblotting procedures. That protein restores both excision repair and UV resistance to mei-9 and mus201 mutants of this organism. These results reveal that the denV gene can compensate for excision-repair defects in two very different eukaryotic mutants, in that the mus201 mutants are typical of excision-deficient mutants in other organisms, whereas the mei-9 mutants exhibit a broad pleiotropism that includes a strong meiotic deficiency. This study permits an extension of the molecular analysis of DNA repair to the germ line of higher eukaryotes. It also provides a model system for future investigations of other well-characterized microbial repair genes on DNA damage in the germ line of this metazoan organism.
Banga
S S
SS
Department of Genetics, University of California, Davis 95616.
Boyd
J B
JB
Valerie
K
K
Harris
P V
PV
Kurz
E M
EM
de Riel
J K
JK
D000818:Animals; D015153:Blotting, Western; D003001:Cloning, Molecular; D004247:DNA / Q000096:biosynthesis; D045647:DNA Glycosylases; D004260:DNA Repair; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D043224:Deoxyribonuclease (Pyrimidine Dimer); D004331:Drosophila melanogaster / Q000235:genetics*; D004706:Endodeoxyribonucleases / Q000235:genetics; D005786:Gene Expression Regulation; D005814:Genes, Viral; D005854:Germ Cells; D006360:Heat-Shock Proteins / Q000235:genetics; D009154:Mutation; D009699:N-Glycosyl Hydrolases / Q000378:metabolism; D010957:Plasmids; D011401:Promoter Regions, Genetic; D013604:T-Phages / Q000235:genetics*; D014170:Transformation, Genetic
D004274:DNA, Recombinant; D006360:Heat-Shock Proteins; D004247:DNA; D004706:Endodeoxyribonucleases; D004262:DNA Restriction Enzymes; D043224:Deoxyribonuclease (Pyrimidine Dimer); D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; C036401:deoxyribopyrimidine endonucleosidase