Endocytosis and recycling of the fibronectin receptor in CHO cells.
An anti-fibronectin receptor monoclonal antibody preferentially labels the leading edges of freshly plated CHO fibroblasts, suggesting that this receptor circulates through the endocytic cycle. Using a new labelling reagent, I show that this receptor is indeed endocytosed at 37 degrees C and then returned to the cell surface. These findings imply that fibronectin receptors are recirculated to the leading edge of a motile cell by the endocytic cycle, and establish that the processes of endocytosis/exocytosis and cell locomotion are intimately linked.
Bretscher
M S
MS
Medical Research Council, Laboratory of Molecular Biology, Cambridge, UK.
D000818:Animals; D002465:Cell Movement; D002478:Cells, Cultured; D004151:Dipeptides; D004705:Endocytosis; D005089:Exocytosis; D005455:Fluorescent Antibody Technique; D007202:Indicators and Reagents; D007700:Kinetics; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic / Q000502:physiology*; D013388:Succinimides
C059298:3,3'-dithiopropionyl 1-sulfosuccinimidyl 1'-glycyl-tyrosine; D004151:Dipeptides; D007202:Indicators and Reagents; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic; D013388:Succinimides