Molecular cloning of the 25 kbp region upstream of exon 0 of the human Ki-ras oncogene and its conservation in transformed mouse NIH 3T3 cells.
Human sequences associated with the Ki-ras oncogene of the mammary tumour cell line, H-466B have been cloned from a tertiary NIH3T3 mouse transfectant. These sequences are located 5' upstream of exon 0 of the Ki-ras oncogene, span over 25 kbp of DNA and are conserved in half of the primary transfectants obtained with the Ki-ras gene of different types of tumours. No gross alterations were observed in the sequences upstream of the Ki-ras gene. The partial or total deletion of these sequences in the other half of primary transformants argues that they are not absolutely required for the transforming activity of the Ki-ras oncogene. The even distribution of the human-mouse junction points in primary transformed mouse cells suggests the absence of a specific region of recombination in the 5' flanking region of Ki-ras.
Courtois
G
G
Laboratoire d'Oncogenèse Moléculaire, Institut Curie, Paris, France.
Lebeau
J
J
Goubin
G
G
D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics; D001943:Breast Neoplasms / Q000235:genetics; D002471:Cell Transformation, Neoplastic; D003001:Cloning, Molecular; D003062:Codon; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D004262:DNA Restriction Enzymes; D004587:Electrophoresis, Agar Gel; D005091:Exons; D011905:Genes, ras; D006801:Humans; D051379:Mice; D009693:Nucleic Acid Hybridization; D014162:Transfection; D014407:Tumor Cells, Cultured
D003062:Codon; D015342:DNA Probes; D004247:DNA; D004262:DNA Restriction Enzymes