Comparison of gas-liquid chromatography and fluorescence polarization immunoassay for therapeutic drug monitoring of flecainide acetate.
A gas chromatographic (GC) method for quantitation of flecainide acetate in human plasma is described and compared with a fluorescence polarization immunoassay (FPIA) for therapeutic drug monitoring. The GC method includes a solid-phase extraction procedure and electron capture detection (ECD) without the need of derivatization. Within-day and between-day coefficients of variation were less than 7% of GC and FPIA. Recovery was between 89-101% for the GC method. Plasma from 36 patients were analysed by both GC and FPIA and the results showed a good correlation (slope = 0.96; intercept = 0.009 micrograms ml-1; r = 0.987).
Stas
C M
CM
Pharmacokinetics Unit, Catholic University of Louvain, Brussels, Belgium.
Jacqmin
P A
PA
Pellegrin
P L
PL
D002849:Chromatography, Gas; D005424:Flecainide / Q000032:analysis* / Q000097:blood; D016231:Fluorescence Polarization Immunoassay; D006801:Humans; D007202:Indicators and Reagents
D007202:Indicators and Reagents; D005424:Flecainide