Detecting giardia lamblia antigen in fecal matter with counterimmuno-electrophoresis in diagnosis of giardiasis.
Feces specimens from 35 patients with parasitologically confirmed giardiasis, 41 with acute gastroenteritis, 23 with acute bacillary dysentery, 40 normal persons as well as 15 jirds experimentally infected with Giardia lamblia were used for detecting parasite antigen by counterimmunoelectrophoresis (CIE). It revealed that 33 (94%) of the 35 patients with giardiasis and 14 (93%) of 15 infected jirds were positive, while the other cases, either normal person or normal jird were negative. CIE was also performed in 4 with giardiasis before and after metronidazole treatment. Prior to metronidazole administration, they were all CIE positive, but from the second day on, all became CIE negative. Apparently, detecting giardia antigen in feces specimens by CIE is not only sensitive in detecting current infection but also helpful in evaluating the effects of antigiardia agents on giardiasis.
Lu
S Q
SQ
Wang
Z Y
ZY
Zhang
Y Q
YQ
Wen
Y
Y
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis*; D003378:Counterimmunoelectrophoresis; D005243:Feces / Q000032:analysis; D005849:Gerbillinae; D005872:Giardia / Q000276:immunology*; D005873:Giardiasis / Q000175:diagnosis* / Q000188:drug therapy; D006801:Humans; D008795:Metronidazole / Q000627:therapeutic use
D000953:Antigens, Protozoan; D008795:Metronidazole