Retroviral insertions in the CB-1/Fim-3 common site of integration activate expression of the Evi-1 gene.
A common retroviral integration site (CB-1) was identified in two IL-3-dependent myeloid leukemia cell lines (DA-3, DA-34). The CB-1 locus was mapped to murine chromosome 3 and was shown to be closely linked to another myeloid common site of viral integrations in myeloid leukemias, Evi-1. A comparison of the CB-1 restriction map with published restriction maps for other common integration sites demonstrated that it was nearly identical to the restriction map of a common site of Friend MuLV integration in myeloid tumors termed Fim-3 (Bordereaux et al. 1987). Genomic clones representing approximately 110 kb of the CB-1 locus and 80 kb of the 5' region of the Evi-1 locus demonstrate no physical overlap of these viral integration sites. Previous studies (Morishita et al. 1988) have shown that retroviral insertions in the Evi-1 locus activate the expression of a gene potentially encoding a 120 kd zinc finger protein. Evi-1 expression is also activated in cell lines with viral integrations in the CB-1 locus. These results demonstrate that the CB-1/Fim-3 and Evi-1 loci constitute a large genomic region in which viral integrations activate the transcription of a new potential myeloid transforming gene.
Bartholomew
C
C
Department of Biochemistry, St. Jude Children's Research Hospital, Memphis, Tennessee 38101.
Morishita
K
K
Askew
D
D
Buchberg
A
A
Jenkins
N A
NA
Copeland
N G
NG
Ihle
J N
JN
D000818:Animals; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D005786:Gene Expression Regulation; D008040:Genetic Linkage; D015151:Immunoblotting; D007377:Interleukin-3 / Q000502:physiology; D007951:Leukemia, Myeloid / Q000235:genetics*; D051379:Mice; D011533:Proviruses / Q000235:genetics
D004251:DNA Transposable Elements; D007377:Interleukin-3