Intracellular localization and DNA-binding properties of human papillomavirus type 18 E6 protein expressed with a baculovirus vector.
The E6 protein of human papillomavirus type 18 (HPV-18) is a putative zinc-finger protein that is expressed in HPV-18-induced genital neoplasias. We have studied the biochemical properties of E6 protein synthesized in large amounts with a baculovirus expression vector. When E6 protein was synthesized in insect cells infected with an E6-expressing baculovirus, the protein was localized to both nuclear and membrane fractions, with half-lives of 4 and 2 h, respectively. Changing the first five amino acids of E6 did not alter the pattern of cellular localization of the protein but dramatically increased the half-life of the nuclear component to longer than 30 h and increased the half-life of the membrane component to 8 h. Although the baculovirus-expressed E6 protein bound to double-stranded DNA with high affinity, no sequence specificity for HPV-18 DNA was detected.
Grossman
S R
SR
Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.
Mora
R
R
Laimins
L A
LA
D000818:Animals; D015153:Blotting, Western; D002458:Cell Fractionation; D002460:Cell Line; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D006207:Half-Life; D006801:Humans; D007304:Insect Viruses / Q000235:genetics; D008667:Metalloproteins / Q000032:analysis* / Q000096:biosynthesis / Q000378:metabolism; D009036:Moths; D027383:Papillomaviridae / Q000235:genetics*; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism; D015032:Zinc
D004268:DNA-Binding Proteins; D008667:Metalloproteins; D011994:Recombinant Proteins; D004247:DNA; D015032:Zinc