A sensitive bioassay for teicoplanin in serum in the presence or absence of other antibiotics.
A teicoplanin bioassay has been developed that is accurate, sensitive, and reliable. A linear relationship is obtained between the diameter of the zone of inhibition and log10 teicoplanin concentration in human serum over the range of 0.15 or 1.25 to 96 micrograms/ml using wells or paper filter disks, respectively. The assay medium devised consists of 50 g BBL Mueller-Hinton II Agar, 30 g NaCl, 8 g CaCl2, and 1.0 g citric acid (monohydrate) per liter of deionized water (resulting pH 5.1 +/- 0.1) and the assay organism Bacillus subtilis ATCC 6633. This system allows the assay of teicoplanin in the presence of commonly used aminoglycosides and in the presence of beta-lactams after inactivation by beta-lactamase. Additionally, it has the potential to be used in the presence of rifampin by using a rifampin-resistant strain of B. subtilis.
Erickson
R C
RC
Merrell Dow Research Institute, Cincinnati, Ohio 45215.
Hildebrand
A R
AR
Hoffman
P F
PF
Gibson
C B
CB
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000097:blood*; D001412:Bacillus subtilis / Q000187:drug effects; D001681:Biological Assay / Q000379:methods; D005069:Evaluation Studies as Topic; D006020:Glycopeptides / Q000097:blood; D006801:Humans; D007769:Lactams; D012293:Rifampin / Q000097:blood; D017334:Teicoplanin
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D006020:Glycopeptides; D007769:Lactams; D017334:Teicoplanin; D012293:Rifampin