Identification of a transforming gene of human papillomavirus type 16.
Previously, we observed sequential two-step alteration, growth stimulation, and progression to a more malignant state in NIH 3T3 cells transfected by human papillomavirus type 16 (HPV-16) DNA. In this study, we prepared a cDNA library from RNA extracted from cells transfected with the HPV-16 DNA and isolated cDNA clones which had growth-stimulating activity. Analysis of these cDNA clones indicated that the E7 open reading frame alone is responsible for inducing both steps of this cell transformation.
Tanaka
A
A
Institute for Virus Research, Kyoto University, Japan.
Noda
T
T
Yajima
H
H
Hatanaka
M
M
Ito
Y
Y
D000818:Animals; D002478:Cells, Cultured / Q000166:cytology / Q000382:microbiology; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005814:Genes, Viral; D006133:Growth Substances / Q000235:genetics*; D009857:Oncogenes; D027383:Papillomaviridae / Q000235:genetics*; D051381:Rats
D006133:Growth Substances; D004247:DNA