title: [Coprecipitation of proteins and DNA-protein complexes with magnesium sarcolysate: research on an association of topoisomerase I and RNA polymerase with DNA].
abstract: A specific cosedimentation of proteins and their complexes with DNA at low temperature (M-band technique) has been demonstrated. Model experiments with reconstituted SV40 DNA-topoisomerase I and SV40 DNA-E. coli RNA polymerase complexes demonstrated the potential and capacities of the method. It allows fractionation of DNA-protein complexes from naked DNA and has greater range, higher reproducibility and lower background values than similar methods.
authors.forename: E S
authors.initials: ES
authors.lastname: Vasetskiĭ
authors.forename: V V
authors.initials: VV
authors.lastname: Bakaev
mesh_terms: D001426:Bacterial Proteins / Q000302:isolation & purification*; D011232:Chemical Precipitation; D003300:Copper / Q000633:toxicity; D004264:DNA Topoisomerases, Type I / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004279:DNA, Viral / Q000302:isolation & purification*; D012321:DNA-Directed RNA Polymerases / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology; D006878:Hydroxides / Q000633:toxicity; D007202:Indicators and Reagents; D008558:Melphalan / Q000494:pharmacology*; D008722:Methods; D013539:Simian virus 40 / Q000201:enzymology; D013696:Temperature; D014764:Viral Proteins / Q000302:isolation & purification*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004279:DNA, Viral; D006878:Hydroxides; D007202:Indicators and Reagents; D014764:Viral Proteins; C001606:cupric hydroxide; D003300:Copper; D012321:DNA-Directed RNA Polymerases; D004264:DNA Topoisomerases, Type I; D008558:Melphalan
vernacular_title: Soosazhdenie belkov i kompleksov DNK--belok s sarkozilatom magniia: issledovanie assotsiatsii topoizomerazy I i RNK polimerazy s DNK.