T helper cells grown with hapten-modified cultured Langerhans' cells produce interleukin 4 and stimulate IgE production by B cells.
Hapten-specific CD4+ T helper (Th) lines generated by repeated stimulation with hapten-modified, cultured Langerhans' cells (cLC) release interleukin (IL 4) (B cell stimulatory factor 1) but not detectable IL 2 into the culture media. The growth of Th cells in response to hapten-modified cLC was inhibited by an anti-IL 4 monoclonal antibody (mAb) but not by mAb to either IL 2 or the p55 chain of the IL 2 receptor. Furthermore, these cells could be stimulated to proliferate by concanavalin A and IL 1. These results indicate that IL 4 is the autocrine growth factor for these Th lines and that IL 1 plays a critical role in their growth. The Th cells exhibited 1,500-10,000 high-affinity IL 4 receptors cell. When cultured with syngeneic, hapten-modified, small resting B cells, Th cells caused specific IgE production of up to 20 ng/10(4) B cells. Thus, IL 4 producing Th lines appear to result from their selective stimulation by cLC, suggesting that T cell responses elicited in this way profoundly influenced the B cell isotype pattern.
Hauser
C
C
Dermatology Branch, National Cancer Institute, Bethesda.
Snapper
C M
CM
Ohara
J
J
Paul
W E
WE
Katz
S I
SI
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology; D001402:B-Lymphocytes / Q000378:metabolism*; D001667:Binding, Competitive; D002450:Cell Communication; D002460:Cell Line; D002478:Cells, Cultured; D003208:Concanavalin A; D003470:Culture Media; D006241:Haptens; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007375:Interleukin-1 / Q000378:metabolism; D007376:Interleukin-2 / Q000032:analysis; D015847:Interleukin-4; D007378:Interleukins / Q000096:biosynthesis* / Q000276:immunology / Q000378:metabolism; D007801:Langerhans Cells / Q000276:immunology / Q000378:metabolism* / Q000502:physiology; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D011948:Receptors, Antigen, T-Cell / Q000032:analysis; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology / Q000378:metabolism* / Q000502:physiology
D000911:Antibodies, Monoclonal; D003470:Culture Media; D006241:Haptens; D007375:Interleukin-1; D007376:Interleukin-2; D007378:Interleukins; D011948:Receptors, Antigen, T-Cell; D003208:Concanavalin A; D015847:Interleukin-4; D007073:Immunoglobulin E