Transient expression of the angiotensin II receptor: a rapid and functional analysis of a calcium-mobilizing seven-transmembrane domain receptor in COS-7 cells.
The mas oncogene/angiotensin II receptor was subcloned into a mammalian expression vector pCDM8 and used to transiently transfect monkey kidney derived COS-7 cells. As a result, the mas transfected COS-7 cells expressed a functional angiotensin II receptor capable of transducing an increase in intracellular Ca2+ following stimulation with angiotensin II. The angiotensin II stimulated changes in Ca2+ could be measured 24 hours after transfection in both a fluorimeter and a fluorescence activated cell sorter. These results describe a rapid method for the functional analysis of the 7-transmembrane domain receptor genes.
McGillis
J P
JP
Howard Hughes Medical Institute, University of California, San Francisco 94143.
Sudduth-Klinger
J
J
Harrowe
G
G
Mitsuhashi
M
M
Payan
D G
DG
D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D003001:Cloning, Molecular; D004837:Epinephrine / Q000494:pharmacology; D015870:Gene Expression; D005822:Genetic Vectors; D000882:Haplorhini; D007668:Kidney; D011945:Receptors, Angiotensin / Q000235:genetics*; D014162:Transfection
D011945:Receptors, Angiotensin; D000804:Angiotensin II; D002118:Calcium; D004837:Epinephrine