title: cDNA cloning and sequencing of phospholamban from pig stomach smooth muscle.
abstract: Phospholamban cDNA from pig stomach smooth muscle was cloned and sequenced. The 737-nucleotide-residue cDNA contained an open reading frame of 156 nucleotide residues encoding a peptide of 52 amino acid residues (Mr 6080). This peptide shares 100% sequence identity with dog cardiac-muscle phospholamban. It differs from rabbit cardiac-muscle and slow-twitch skeletal-muscle phospholamban only at position 2, which is a glutamic acid residue in rabbit phospholamban, but an aspartic acid residue in the pig smooth-muscle protein. Northern-blot analysis reveals the presence of several phospholamban mRNAs in smooth muscle, but a 900-nucleotide-residue and a 2800-nucleotide-residue transcript predominate.
authors.affiliation: Laboratorium voor Fysiologie, Katholieke Universiteit Leuven, Campus Gasthuisberg, Belgium.
authors.forename: H
authors.initials: H
authors.lastname: Verboomen
authors.forename: F
authors.initials: F
authors.lastname: Wuytack
authors.forename: J A
authors.initials: JA
authors.lastname: Eggermont
authors.forename: S
authors.initials: S
authors.lastname: De Jaegere
authors.forename: L
authors.initials: L
authors.lastname: Missiaen
authors.forename: L
authors.initials: L
authors.lastname: Raeymaekers
authors.forename: R
authors.initials: R
authors.lastname: Casteels
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002135:Calcium-Binding Proteins; D003001:Cloning, Molecular; D015342:DNA Probes; D004270:DNA, Circular; D008969:Molecular Sequence Data; D009130:Muscle, Smooth / Q000201:enzymology*; D013552:Swine; D000099211:Phospholamban
chemical_list: D000251:Adenosine Triphosphatases; D002135:Calcium-Binding Proteins; D015342:DNA Probes; D004270:DNA, Circular; D000099211:Phospholamban