Regulation of antigen presentation. II. Anti-Ig and IL-2 induce IL-1 production by murine splenic B cells.
Murine splenic B cells did not constitutively express IL-1 activity. After culture with anti-Ig and T cell-conditioned media and then fixation, B cells expressed membrane IL-1 and were able to stimulate growth of the IL-1-dependent T cell clone D10. Expression of membrane IL-1 required stimulation of B cells for 2 days before fixation. Significant IL-1 activity was detectable in freeze-thaw lysates of identical B cell preparations by 12 h. B cells also released IL-1 into the culture media. In situ hybridization studies by using probes to murine IL-1 alpha and IL-1 beta genes supported these observations. Thus, messenger RNA for IL-1 alpha and IL-1 beta rose in parallel, were detected between 6 and 24 h of culture, and declined to low levels by 30 h. Despite the presence of mRNA for IL-1 alpha and IL-1 beta, only IL-1 alpha had functional activity as determined by the use of a mAb to IL-1 alpha. IL-2 was found to be an essential component of the T cell-derived supernatant. Although IL-4 or TNF did not induce significant B cell IL-1 expression, they both caused a modest, but reproducible enhancement when added in combination with IL-2. IFN-gamma, by contrast, partially inhibited IL-1 induction.
Hawrylowicz
C M
CM
Department of Pathology, Washington University School of Medicine, St. Louis, MO 63110.
Duncan
L M
LM
Fuhlbrigge
R C
RC
Unanue
E R
ER
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000502:physiology*; D000938:Antigen-Presenting Cells / Q000276:immunology*; D001402:B-Lymphocytes / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D002458:Cell Fractionation; D003470:Culture Media; D004338:Drug Combinations; D005260:Female; D007375:Interleukin-1 / Q000032:analysis / Q000096:biosynthesis* / Q000502:physiology; D007376:Interleukin-2 / Q000494:pharmacology*; D008222:Lymphokines / Q000494:pharmacology; D008565:Membrane Proteins / Q000032:analysis / Q000096:biosynthesis; D051379:Mice; D008809:Mice, Inbred C3H; D008808:Mice, Inbred CBA; D009693:Nucleic Acid Hybridization; D013154:Spleen; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D003470:Culture Media; D004338:Drug Combinations; D007375:Interleukin-1; D007376:Interleukin-2; D008222:Lymphokines; D008565:Membrane Proteins