title: Complement-fixing antibodies against rotaviruses in healthy children in the Czech Socialist Republic and People's Democratic Republic of Yemen.
abstract: Levels of complement-fixing antibodies against rotaviruses were evaluated in the sera of 900 healthy children aged 1-9 years 300 sera were collected in the People's Democratic Republic of Yemen in September-October 1985, 300 sera were obtained in the Czech Socialist Republic in the same period and another 300 also in the Czech Socialist Republic in September-October 1986. The latter two groups were investigated in the framework of immunological surveys. A complement-fixation antigen was prepared from a simian strain of the rotavirus type SA-11 in a tissue cell line MA-104. The sera from Yemen featured lower mean titres in the age groups and thus the lowest overall titre. As the antibody titre increased, the portion of seropositive sera from Yemen declined by far more rapidly than in the Czech children, where it remained virtually the same. The sera from Yemen showed the lowest negative rate and lowest ratio of high titres. The antibody titre of 1:64 and higher was not detected in children from Yemen, while they occurred in the two groups of Czech children. There was no correlation between antibody titres and probands' sex, nor was there linear dependence of titre magnitude on age. The mean positivity rate in each group as assessed by the antibody titres was the lowest in the sera from Yemen. The percentage of positive sera in all age groups was higher in the Czech children with the exception of children from Yemen aged 6 and 9 years. The aim of the present study was to evaluate the antibody status in infant populations and thus expand knowledge of rotavirus epidemiology.
authors.affiliation: Institute of Hygiene and Epidemiology, Prague, Czechoslovakia.
authors.forename: Z
authors.initials: Z
authors.lastname: Kucharská
authors.forename: L
authors.initials: L
authors.lastname: Danes
authors.forename: S S
authors.initials: SS
authors.lastname: Dobahi
authors.forename: K
authors.initials: K
authors.lastname: Kopecký
authors.forename: E
authors.initials: E
authors.lastname: Svandová
mesh_terms: D000367:Age Factors; D000914:Antibodies, Viral / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D003168:Complement Fixation Tests; D003604:Czechoslovakia; D006801:Humans; D007223:Infant; D012401:Rotavirus / Q000276:immunology*; D012737:Sex Factors; D013997:Time Factors; D015006:Yemen
chemical_list: D000914:Antibodies, Viral