[Tissue culture and estrogen, to clarify the roles of estrone sulfate].
Estrone sulfate (E1-S) has been shown to be quantitatively the most important estrogen in peripheral blood. But, the physiological and/or pathological role of E1-S is not yet clarified. At present, we tried to clarify it using tissue cultures. In tissue cultures of human endometrium, secretory endometrium showed higher activity of estrone sulfatase (E1----E1-S) than proliferative endometrium. Progesterone added in the medium induced an increase of estrone sulfotransferase in the proliferative endometrium. The results suggest a reducing effect of estrogen by progesterone in secretory endometrium in physiological conditions. Estrogen dependent malignant tumors (breast cancer, endometrial cancer) have high estrone sulfatase. It converts E1-S to E1 (----E2) which are abundant in these tumors. Ishikawa cell line increased estrone sulfotransferase activity with progesterone, somewhat like the physiological conditions. From out study in vivo, there is a possibility of some ameliorative effects of E1-S on the central nervous system of patients with senile dementia (Alzheimer's type). Effects of E1-S on central nerves were investigated using tissue cultures.
Honjo
H
H
Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Japan.
Urabe
M
M
Naitoh
K
K
Ogino
Y
Y
Kitawaki
J
J
Yasuda
J
J
Yamamoto
T
T
Okada
H
H
D000544:Alzheimer Disease / Q000188:drug therapy; D001943:Breast Neoplasms / Q000378:metabolism; D046508:Culture Techniques; D016889:Endometrial Neoplasms / Q000378:metabolism; D004717:Endometrium / Q000378:metabolism*; D004960:Estradiol Dehydrogenases / Q000378:metabolism; D004970:Estrone / Q000031:analogs & derivatives* / Q000378:metabolism / Q000502:physiology / Q000627:therapeutic use; D005260:Female; D006801:Humans; D011374:Progesterone / Q000502:physiology; D013429:Sulfatases / Q000378:metabolism; D015238:Sulfotransferases; D013466:Sulfurtransferases / Q000378:metabolism
D004970:Estrone; D011374:Progesterone; D004960:Estradiol Dehydrogenases; D013466:Sulfurtransferases; D015238:Sulfotransferases; C020122:estrone sulfotransferase; D013429:Sulfatases; C020121:estrone sulfatase; C017296:estrone sulfate