Oxidative metabolic activity of boar spermatozoa: a system for assessing anti-glycolytic activity of potential inhibitors in vitro.
The oxidative metabolic capability of mature boar spermatozoa has been determined in vitro. The high rate of oxidation of fructose, glucose, glycerol, glycerol-3-phosphate and lactate to CO2 and the optimization of incubation conditions indicates that these cells could constitute a model system for investigating the anti-glycolytic activity of potential male anti-fertility agents. The effects of several chemical agents on the oxidative metabolism of boar spermatozoa are reported.
Jones
A R
AR
Department of Biochemistry, University of Sydney, N.S.W., Australia.
Chantrill
L A
LA
D000096:Acetone / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D002728:Chlorohydrins / Q000494:pharmacology; D005632:Fructose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D066298:In Vitro Techniques; D007773:Lactates / Q000378:metabolism / Q000494:pharmacology; D008297:Male; D010084:Oxidation-Reduction; D011773:Pyruvates / Q000378:metabolism; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism*; D013552:Swine; D000517:alpha-Chlorohydrin / Q000031:analogs & derivatives / Q000494:pharmacology
D002728:Chlorohydrins; D007773:Lactates; D011773:Pyruvates; D000096:Acetone; D002245:Carbon Dioxide; C007000:3-chlorolactate; C064190:alpha-chlorohydrin-1-phosphate; D005632:Fructose; C064189:3-iodo-1-hydroxypropan-2-one; C009721:CL 88236; D000517:alpha-Chlorohydrin; D005990:Glycerol