A long-acting heat-stable enterotoxin analog of enterotoxigenic Escherichia coli with a single D-amino acid.
A heat-stable enterotoxin (STp) consisting of 18 amino acid residues including 6 half-cystine residues is produced by a porcine strain of enterotoxigenic Escherichia coli. Analogs of STp with replacements of single residues at each from positions 5 to 17 by the corresponding D-amino acid residue were synthesized by a solid-phase method. Of these analogs, [D-Cys5]-STp[5-17] showed the same biological properties as STp[5-17]. Moreover, its activity to cause fluid accumulation in suckling mouse lasts more than 24 hours, whereas the activity of STp[5-17] decreases after 6-10 hours. These results indicate that the action of the analog [D-Cys5]-STp[5-17] is strongly agonistic to that of the native ST.
Kubota
H
H
Institute for Protein Research, Osaka University, Japan.
Hidaka
Y
Y
Ozaki
H
H
Ito
H
H
Hirayama
T
T
Takeda
Y
Y
Shimonishi
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D000833:Animals, Suckling; D001427:Bacterial Toxins / Q000138:chemical synthesis / Q000378:metabolism / Q000633:toxicity*; D001826:Body Fluids / Q000473:pathology; D004220:Disulfides; D004768:Enterotoxins / Q000138:chemical synthesis / Q000378:metabolism / Q000633:toxicity*; D004926:Escherichia coli / Q000472:pathogenicity*; D029968:Escherichia coli Proteins; D006162:Guanylate Cyclase; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000473:pathology; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000032:analysis; D000074261:Receptors, Enterotoxin; D050705:Receptors, Guanylate Cyclase-Coupled; D018000:Receptors, Peptide
D001427:Bacterial Toxins; D004220:Disulfides; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D011956:Receptors, Cell Surface; D018000:Receptors, Peptide; C034151:heat stable toxin (E coli); D006162:Guanylate Cyclase; D000074261:Receptors, Enterotoxin; D050705:Receptors, Guanylate Cyclase-Coupled