Study of a monoclonal antibody specific to epithelial cells of mammalian thymuses.
Hybridomas between spleen cells from BALB/c mice and Sp2/O-Ag14 mycloma cell line are performed by PEG fusion. An in vitro booster is realized with human thymic reticulo-epithelial cell cultures as antigen. A monoclonal antibody (3H9) directed against the thymic epithelium and stratified epithelia of mammals has been obtained and characterized.
Parvaz
P
P
Laboratoire d'Immunologie UFR Alexis Carrel, Lyon, France.
Auger
C
C
Giraud
F
F
Fabien
N
N
Faure
J R
JR
Monier
J C
JC
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D004848:Epithelium / Q000276:immunology; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D007145:Immunoglobulin kappa-Chains / Q000276:immunology; D008322:Mammals / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D009928:Organ Specificity; D013045:Species Specificity; D013950:Thymus Gland / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007075:Immunoglobulin M; D007145:Immunoglobulin kappa-Chains