title: Inhibition of poly(ADP-ribosyl)ation does not prevent lymphocyte entry into the cell cycle.
abstract: The enzyme poly(ADP-ribosyl)transferase (ADPRT) becomes activated soon after a mitogenic stimulus is applied to lymphocyte cultures. It has also been reported that ADPRT inhibitors prevent cell proliferation when added to cultures at the same time as the mitogen. While this has been ascribed to the need to seal physiologically present DNA strand breaks before cells enter S phase, the presence of DNA strand breaks in quiescent human lymphocytes has been recently questioned. We demonstrate here that non-toxic concentrations of ADPRT inhibitors do not affect lymphocyte blastization and proliferation, as measured by thymidine incorporation and cytofluorimetry. We therefore suggest that ADPRT activation is required for late functions which are not needed for cell cycle progression.
authors.affiliation: Istituto di Istologia ed Embriologia Gen., Bologna, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Marini
authors.forename: G
authors.initials: G
authors.lastname: Zunica
authors.forename: D
authors.initials: D
authors.lastname: Monti
authors.forename: A
authors.initials: A
authors.lastname: Cossarizza
authors.forename: C
authors.initials: C
authors.lastname: Ortolani
authors.forename: C
authors.initials: C
authors.lastname: Franceschi
mesh_terms: D001549:Benzamides / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects; D000431:Ethanol / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000166:cytology / Q000502:physiology*; D009536:Niacinamide / Q000494:pharmacology; D009702:Nucleoside Diphosphate Sugars / Q000502:physiology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011064:Poly Adenosine Diphosphate Ribose / Q000502:physiology*; D000067856:Poly(ADP-ribose) Polymerase Inhibitors
chemical_list: D001549:Benzamides; D009702:Nucleoside Diphosphate Sugars; D010835:Phytohemagglutinins; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D009536:Niacinamide; D011064:Poly Adenosine Diphosphate Ribose; D000431:Ethanol; C025160:3-aminobenzamide