An abundant U6 snRNP found in germ cells and embryos of Xenopus laevis.
The particle state of U snRNPs was analyzed in oocytes, eggs, embryos and testes from Xenopus laevis. In each case both the relative abundance and the composition of some U snRNPs were found to differ from that of somatic cells. U2 and U6 snRNPs were the most prominent U snRNPs in germ cells and early embryos. In particular, the concentration of U6 snRNA was 10-20 times higher than that of U4 snRNA. Most of the U6 snRNA was not associated with U4 snRNA and migrated on sucrose gradients as a U6 snRNP. The structure of this novel U snRNP was analyzed. A single protein of 50 kd was copurified with U6 snRNPs by a combination of gradient fractionation, immunodepletion with anti-Sm antibodies and immunoprecipitation with anti-6-methyl adenosine antibodies. Although the U6 snRNP did not contain Sm proteins it migrated into the nucleus when U6 snRNA was injected into the cytoplasm of oocytes. Two U6 snRNA elements have been identified. The first is essential for nuclear migration in oocytes, but not for the formation of U4/6 snRNPs in vitro and might be the binding site of a U6-specific protein. The second element was required for interaction with U4 snRNPs but not for nuclear targeting.
Hamm
J
J
EMBL Heidelberg, FRG.
Mattaj
I W
IW
D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D015152:Blotting, Northern; D002467:Cell Nucleus / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000502:physiology*; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D009865:Oocytes / Q000502:physiology*; D010063:Ovum / Q000502:physiology*; D012342:RNA, Small Nuclear / Q000302:isolation & purification*; D012261:Ribonucleoproteins / Q000302:isolation & purification*; D017411:Ribonucleoproteins, Small Nuclear; D013737:Testis / Q000502:physiology*; D014982:Xenopus laevis
D000911:Antibodies, Monoclonal; D012342:RNA, Small Nuclear; D012261:Ribonucleoproteins; D017411:Ribonucleoproteins, Small Nuclear