Cloning and sequence analysis of a cDNA encoding the Rieske iron-sulfur protein of rat mitochondrial cytochrome bc1 complex.
We have isolated a cDNA clone for the Rieske iron-sulfur protein of rat cytochrome bc1 complex, by screening a rat liver cDNA expression library using antiserum directed against the corresponding protein of bovine. The amino acid sequence deduced from the nucleotide sequence of the cDNA indicated that the mature polypeptide of the rat protein consists of 196 amino acid residues with a molecular weight of 21,465, and that it is formed as a precursor with an amino-terminal extension. Northern blot analysis indicated that rat liver possibly contains different sizes of mRNAs for the Rieske iron-sulfur protein, and Southern blot analysis demonstrated that rats and mice possess a single gene for this protein.
Nishikimi
M
M
Department of Biomedical Chemistry, Faculty of Medicine, University of Nagoya, Japan.
Hosokawa
Y
Y
Toda
H
H
Suzuki
H
H
Ozawa
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D015342:DNA Probes; D004262:DNA Restriction Enzymes; D014450:Electron Transport Complex III / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000032:analysis; D006801:Humans; D007506:Iron-Sulfur Proteins / Q000235:genetics*; D008667:Metalloproteins / Q000235:genetics*; D051379:Mice; D008930:Mitochondria, Liver / Q000032:analysis*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D011498:Protein Precursors; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats
D015342:DNA Probes; D007506:Iron-Sulfur Proteins; D008667:Metalloproteins; D011498:Protein Precursors; D012333:RNA, Messenger; C037989:Rieske iron-sulfur protein; D004247:DNA; D004262:DNA Restriction Enzymes; D014450:Electron Transport Complex III