title: Postnatal development of thiamine metabolism in rat brain.
abstract: The activities of thiamine diphosphatase (TDPase), thiamine triphosphatase (TTPase), and thiamine pyrophosphokinase and the contents of thiamine and its phosphate esters were determined in rat brain cortex, cerebellum, and liver from birth to adulthood. Microsomal TTPase activity in the cerebral cortex and cerebellum increased from birth to 3 weeks, whereas that in the liver did not change during postnatal development. Microsomal TDPase activity in the cerebral cortex showed a transient increase at 1-2 weeks, but that in the cerebellum did not change during development. In contrast to the activity of the brain enzyme, that of liver microsomal TDPase increased stepwise after birth. Thiamine pyrophosphokinase activity in the cerebellum increased from birth to 3 weeks and then decreased, whereas that in the cerebral cortex and liver showed less change during development. TDP and thiamine monophosphate (TMP) levels increased after birth and plateaued at 3 weeks whereas TTP and thiamine levels showed little change during development in the cerebral cortex and cerebellum. The contents of thiamine and its phosphate esters in the liver showed more complicated changes during development. It is concluded that thiamine metabolism in the brain changes during postnatal development in a different way from that in the liver and that the development of thiamine metabolism differs among brain regions.
authors.affiliation: Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Matsuda
authors.forename: T
authors.initials: T
authors.lastname: Doi
authors.forename: H
authors.initials: H
authors.lastname: Tonomura
authors.forename: A
authors.initials: A
authors.lastname: Baba
authors.forename: H
authors.initials: H
authors.lastname: Iwata
mesh_terms: D017766:Acid Anhydride Hydrolases; D000375:Aging / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000254:growth & development* / Q000378:metabolism; D002531:Cerebellum / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D008861:Microsomes / Q000201:enzymology; D010710:Phosphates / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013836:Thiamin Pyrophosphokinase / Q000378:metabolism; D013837:Thiamin-Triphosphatase / Q000378:metabolism; D013831:Thiamine / Q000378:metabolism*
chemical_list: D010710:Phosphates; D013836:Thiamin Pyrophosphokinase; D010744:Phosphoric Monoester Hydrolases; D017766:Acid Anhydride Hydrolases; D013837:Thiamin-Triphosphatase; C023837:nucleoside-diphosphatase; D013831:Thiamine