Characterization of a monoclonal antibody which inhibits the biological activity of human chorionic gonadotrophin in human corpora lutea.
In the present study a murine monoclonal antibody (MCA), directed against human chorionic gonadotrophin (HCG), was investigated in vitro utilizing human corpora lutea (CL). The CL were excised from women of fertile age undergoing various gynaecological operations. The CL specimens were cut into pieces and incubated in standard medium for 2 or 4 h in the presence of HCG, luteinizing hormone (HLH) or prostaglandin (PG) E2 alone or in combination with the MCA directed against HCG. After incubation, the tissue levels of cyclic adenosine 3',5' monophosphate (cAMP) and the media contents of progesterone (P) were determined. HCG, HLH and PGE2 stimulated both cAMP and P formation in CL of all ages, while the MCA alone had no effect on these parameters. The MCA, however, effectively counteracted the stimulatory effect of HCG on both cAMP and P formation on a 1:1 molar basis. This antagonistic effect was clearly reversible and could be overcome by increasing the HCG concentrations. The stimulatory effect of HLH and PGE2, on the other hand, was not influenced by the antibody, even when administered at high concentrations. These in-vitro data show that the MCA studied effectively counteracts the stimulatory effect of HCG on human luteal tissue with high specificity.
Hahlin
M
M
Department of Obstetrics and Gynaecology, University of Göteborg, Sweden.
Lindblom
B
B
Schuurs
A
A
Kloosterboer
H
H
Hamberger
L
L
D000328:Adult; D000911:Antibodies, Monoclonal / Q000032:analysis*; D006063:Chorionic Gonadotropin / Q000037:antagonists & inhibitors* / Q000276:immunology / Q000494:pharmacology; D003338:Corpus Luteum / Q000032:analysis / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D000242:Cyclic AMP / Q000096:biosynthesis; D015232:Dinoprostone / Q000494:pharmacology; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007986:Luteinizing Hormone / Q000494:pharmacology; D011374:Progesterone / Q000032:analysis / Q000096:biosynthesis
D000911:Antibodies, Monoclonal; D006063:Chorionic Gonadotropin; D011374:Progesterone; D007986:Luteinizing Hormone; D000242:Cyclic AMP; D015232:Dinoprostone