A genomic clone of Zfy-1 from a YDOM mouse strain detects post-meiotic gene expression of Zfy in testes.
In order to obtain a genomic clone of Zfy-1 from a Y chromosome of Mus musculus domesticus (YDOM) origin, we cloned size-fractionated SJL/J DNA in EMBL-4 and selected colonies which hybridized to pDP1007, a human zinc finger Y clone. The specificity of the clone in hybridizations to mouse and human DNA and partial sequencing confirmed that the clone (subcloned as pGZfy1D) was of Zfy-1 origin. Studies on the expression during testicular development of mRNAs hybridizing to the clone suggested that the gene is expressed post-meiotically.
Kalikin
L M
LM
Cellular and Molecular Biology Program, University of Michigan, Ann Arbor.
Fujimoto
H
H
Witt
M P
MP
Verga
V
V
Erickson
R P
RP
D000375:Aging; D000818:Animals; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D008297:Male; D008540:Meiosis; D008667:Metalloproteins / Q000235:genetics*; D051379:Mice; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013090:Spermatocytes / Q000378:metabolism; D013091:Spermatogenesis; D013737:Testis / Q000254:growth & development / Q000378:metabolism*; D014998:Y Chromosome
D004268:DNA-Binding Proteins; D008667:Metalloproteins; D012333:RNA, Messenger