title: Stimulation of DNA chain initiation by a protein factor (NPF-1) from rat liver of different ages.
abstract: DNA Polymerase-alpha/primase complexes have been isolated from human neuroblastoma IMR-32, embryonic chicken brains (ECB) and rat prostate tumor PA-3 cells. In the presence of (NH4)2SO4 the major part (90%) of primase activity is released from the Pol-alpha/primase complex. A novel hydrophobic interaction column was used for purification of the primase from PA-3 cells. A nuclear protein factor (NPF-1) that stimulates DNA pol-alpha/primase activity has been purified from rat liver of various ages (3-6 months). The nuclear protein factor which only stimulates the primase activity is under investigation. The monoclonal antibodies (SJK 132-20 and 237-71) were used to detect DNA pol-alpha polypeptides from 11- to 19-day-old embryonic chicken brains.
authors.affiliation: Department of Chemistry, University of Notre Dame, IN 46556.
authors.forename: S
authors.initials: S
authors.lastname: Basu
authors.forename: A T
authors.initials: AT
authors.lastname: Rosado
authors.forename: S
authors.initials: S
authors.lastname: Takada
authors.forename: S
authors.initials: S
authors.lastname: Ray
authors.forename: K
authors.initials: K
authors.lastname: Das
authors.forename: I
authors.initials: I
authors.lastname: Suzuki
authors.forename: A P
authors.initials: AP
authors.lastname: Seve
mesh_terms: D000375:Aging; D000546:Amanitins / Q000378:metabolism; D000818:Animals; D002843:Chromatin / Q000302:isolation & purification; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D004265:DNA Helicases / Q000378:metabolism; D019915:DNA Primase; D004261:DNA Replication; D004259:DNA-Directed DNA Polymerase / Q000097:blood / Q000378:metabolism; D008099:Liver / Q000201:enzymology* / Q000378:metabolism; D012316:RNA Nucleotidyltransferases / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism; D051381:Rats
chemical_list: D000546:Amanitins; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D019915:DNA Primase; D012316:RNA Nucleotidyltransferases; D004259:DNA-Directed DNA Polymerase; D004265:DNA Helicases