title: Characterization of integrated human papillomavirus type 11 DNA in primary and metastatic tumors from a renal transplant recipient.
abstract: A primary perianal squamous cell carcinoma and two metastatic tumors from a renal transplant recipient with a previous history of condyloma acuminatum were analyzed by filter hybridization for the presence of human papillomavirus (HPV) DNA. Each of the DNA extracts from these three tissues was found to contain HPV DNA. Stringent hybridization and restriction endonuclease analysis identified this viral DNA as HPV 11 related, which largely comigrated with cellular DNA, suggesting the presence of integrated viral DNA. Each DNA extract was analyzed by two-dimensional gel electrophoresis, which separates circular and linear forms of DNA and can demonstrate linear viral DNA, which comigrated with high molecular weight linear cellular DNA, thus implying viral integration. In all three cases the vast majority of viral DNA was found to comigrate with linear DNA; in addition, a significant portion comigrated with high molecular weight cellular DNA, suggesting the presence of integrated viral DNA in these tumors. Restriction endonuclease analysis of high molecular weight cellular DNA from each of these tumors revealed identical banding patterns, indicating that the integration site in each tissue is identical and, therefore, that all three tumors most likely originated from a single clonal event. These molecular results are presented in light of the clinical history of this patient with a histologically "low grade," but biologically aggressive, squamous cell carcinoma and suggest that HPV 11 may be associated with the initiation of malignant epithelial neoplasms.
authors.affiliation: Institute of Human Genetics, University of Minnesota Medical School, Minneapolis 55455.
authors.forename: D A
authors.initials: DA
authors.lastname: Manias
authors.forename: R S
authors.initials: RS
authors.lastname: Ostrow
authors.forename: R C
authors.initials: RC
authors.lastname: McGlennen
authors.forename: R D
authors.initials: RD
authors.lastname: Estensen
authors.forename: A J
authors.initials: AJ
authors.lastname: Faras
mesh_terms: D000328:Adult; D001005:Anus Neoplasms / Q000382:microbiology*; D002294:Carcinoma, Squamous Cell / Q000382:microbiology*; D004279:DNA, Viral / Q000032:analysis*; D006801:Humans; D007108:Immune Tolerance; D016030:Kidney Transplantation; D008297:Male; D009362:Neoplasm Metastasis; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000235:genetics*
chemical_list: D004279:DNA, Viral