S-100 antigen and calmodulin in human leukemic cells.
Both S-100 antigen and calmodulin were shown in normal lymphocytes with S-100 being decreased in lymphocytic leukemia cells. Although small amounts of S-100 antigen and calmodulin were shown in acute myeloblastic leukemia cells, they could not be detected in normal granulocytes. In lymphoblastic leukemia, S-100 antigen levels in T-cell leukemia cells were higher than in B-cell leukemia cells, while calmodulin was decreased in chronic leukemia cells. In mitogen-stimulated lymphocytes, the levels of S-100 antigen were decreased, while those of calmodulin were either increased or unchanged. Calcium-dependent cyclic nucleotide phosphodiesterase was highest in acute lymphoblastic leukemia. These data suggest, therefore, that calcium ions may play a role in the proliferation, differentiation or leukemic change in lymphocytes and, hence, that measurement of calcium binding proteins may be useful in the investigation of leukemia cells or lymphocytes.
Wada
H
H
2nd Department of Internal Medicine, Mie University School of Medicine, Japan.
Shirakawa
S
S
Yamakado
T
T
Ohkubo
S
S
Tanaka
T
T
Yamamoto
T
T
Hidaka
H
H
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000097:blood; D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000097:blood; D002147:Calmodulin / Q000032:analysis*; D002454:Cell Differentiation; D002455:Cell Division; D006098:Granulocytes / Q000032:analysis; D006801:Humans; D007938:Leukemia / Q000097:blood*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000032:analysis*; D009418:S100 Proteins / Q000032:analysis*
D002147:Calmodulin; D009418:S100 Proteins; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D015106:3',5'-Cyclic-GMP Phosphodiesterases