title: Free gp70 from FeLV: enrichment from cell culture fluid by ferric oxide-agarose chromatography.
abstract: A new chromatographic material based on beads of macroporous crosslinked agarose containing ferric oxide particles was used for enrichment of gp70--the envelope glycoprotein of feline leukemia virus (FeLV). Free gp70 was purified from cell culture fluid in one step with a recovery of 50 to 60% and a purification of about 60 times. The described procedure is a suitable first step for the purification of gp70 from large volumes of cell culture fluid.
authors.affiliation: Institute of Biochemistry, Uppsala University, Sweden.
authors.forename: I
authors.initials: I
authors.lastname: Zelikman
authors.forename: L
authors.initials: L
authors.lastname: Akerblom
authors.forename: S
authors.initials: S
authors.lastname: Hjertèn
authors.forename: B
authors.initials: B
authors.lastname: Morein
mesh_terms: D000818:Animals; D002415:Cats; D002847:Chromatography, Agarose; D003470:Culture Media; D005290:Ferric Compounds; D015151:Immunoblotting; D007525:Isoelectric Focusing; D005256:Leukemia Virus, Feline / Q000032:analysis*; D012191:Retroviridae Proteins / Q000302:isolation & purification*; D015682:Retroviridae Proteins, Oncogenic; D014407:Tumor Cells, Cultured / Q000032:analysis; D014759:Viral Envelope Proteins / Q000302:isolation & purification*
chemical_list: D003470:Culture Media; D005290:Ferric Compounds; D012191:Retroviridae Proteins; D015682:Retroviridae Proteins, Oncogenic; D014759:Viral Envelope Proteins; C000499:ferric oxide