Comparison of five enzyme immunoassays, electron microscopy, and latex agglutination for detection of rotavirus in fecal specimens.
Five different enzyme immunoassays, electron microscopy, and latex agglutination (Slidex; bioMerieux) were compared for the rapid detection of human rotavirus in fecal specimens. The enzyme immunoassay using rotavirus polyclonal antiserum (Dakopatts) with simple in-house modifications was shown by the use of confirmatory tests to be the most sensitive and specific procedure.
Kok
T W
TW
Division of Medical Virology, Institute of Medical and Veterinary Science, Adelaide, Australia.
Burrell
C J
CJ
D005243:Feces / Q000382:microbiology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007841:Latex Fixation Tests; D008854:Microscopy, Electron; D012401:Rotavirus / Q000302:isolation & purification*