A DNA probe specific for L. monocytogenes in the genus Listeria.
Two DNA fragments encoding part of the listeriolysin O of Listeria monocytogenes have been used as probes in Southern blot experiments to detect the presence of the gene in the genus Listeria. Under stringent conditions, and using a fragment internal to the gene, only L. monocytogenes reacted with the probe.
Chenevert
J
J
Department of Biochemistry and Biophysics, University of California School of Medicine, San Francisco.
Mengaud
J
J
Gormley
E
E
Cossart
P
P
D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins; D015139:Blotting, Southern; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D006360:Heat-Shock Proteins / Q000235:genetics; D006460:Hemolysin Proteins / Q000235:genetics; D008087:Listeria / Q000235:genetics*; D008089:Listeria monocytogenes / Q000235:genetics* / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping; D013045:Species Specificity
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D015342:DNA Probes; D004269:DNA, Bacterial; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; C038705:hlyA protein, Listeria monocytogenes