Diagnostic avail of a biotinylated DNA probe made from the XbaI E fragment of human cytomegalovirus genome.
A biotinylated DNA probe has been constructed using the Xba I E fragment of human cytomegalovirus (CMV) genome. This fragment contains a genomic region which is transcribed immediately after infection. The probe proved useful in detecting CMV genome directly in pathological materials and both immediate early viral transcripts and virus genome in infected tissue cultures. The results obtained indicate very good sensitivity and specificity of the probe, suggesting its possible use in routine diagnosis of CMV infection.
Ripalti
A
A
Istituto di Microbiologia dell'Università di Bologna, Policlinico S. Orsola, Italy.
Trevisani
B
B
Landini
M P
MP
La Placa
M
M
D002460:Cell Line; D003429:Cross Reactions; D003587:Cytomegalovirus / Q000235:genetics* / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis*; D015252:Deoxyribonucleases, Type II Site-Specific; D006801:Humans; D009693:Nucleic Acid Hybridization; D011237:Predictive Value of Tests; D015183:Restriction Mapping; D014158:Transcription, Genetic
D015342:DNA Probes; D004279:DNA, Viral; C025580:endodeoxyribonuclease XBAI; D015252:Deoxyribonucleases, Type II Site-Specific