Effect of recombinant hybrid human interferon on replication and morphogenesis of HSV-1 in monkey cells.
Human recombinant alpha interferon, A/D, significantly reduced the replication and cell fusion induced by herpes simplex virus type 1 in monkey cells. Thin-section electron microscopy of interferon-treated monkey cells showed distinct assembly of nucleocapsids within the nucleus. Analysis of virus-specific proteins by the immunoblot technique confirmed that A/D interferon had no significant effect on the expression of major nucleocapsid proteins, although the expression of glycoproteins B and D was reduced in interferon-treated cells. The possibility of an interferon-induced block at a late stage in virus morphogenesis is discussed.
Chatterjee
S
S
Department of Pediatrics, University of Alabama, Birmingham 35294.
Whitley
R J
RJ
D000818:Animals; D002213:Capsid / Q000648:ultrastructure; D002459:Cell Fusion / Q000187:drug effects; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D007370:Interferon Type I / Q000494:pharmacology*; D009024:Morphogenesis / Q000187:drug effects; D011994:Recombinant Proteins; D018139:Simplexvirus / Q000187:drug effects* / Q000254:growth & development; D014758:Viral Core Proteins / Q000648:ultrastructure; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication / Q000187:drug effects*
D007370:Interferon Type I; D011994:Recombinant Proteins; D014758:Viral Core Proteins; D014764:Viral Proteins