title: Bovine leukemia virus (BLV)-infected B-cells express a marker similar to the CD5 T cell marker.
abstract: In the course of generating monoclonal antibodies to bovine thymus-dependent differentiation antigens, we were able to characterize an antibody, termed 8C11, that detects an antigen shared by a majority of thymocytes and peripheral T cells (in blood and thymus-dependent area of spleen and lymph-nodes), but undetectable on normal B cells. However, this antibody was reactive with B cells from cows infected with bovine leukemia virus (BLV). These BLV-infected B cells were found to express simultaneously high concentrations of both surface IgM and 8C11-detected antigen. The antigen recognized by this antibody was shown to be a 67.5 kDa molecule. Because similar findings have been made on mouse myelomas and on human chronic leukemia cells, where this antigen was considered to be the equivalent of mouse Ly-1 antigen and human Leu-1 or CD5 antigen, the T cell antigen detected on BLV-infected cells could be the bovine counterpart of the CD5 antigen. By another way, it has been found that the CD5 T cell antigen is also present on a minor subpopulation of B cells in the spleen but not in the blood. We suggest that in the bovine a similar B cell subpopulation should be the BLV target and expand as a consequence of viral insertion, leading to the persistent lymphocytosis observed on BLV-infected animals.
authors.affiliation: Department of Immunology, University of Namur, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Depelchin
authors.forename: J J
authors.initials: JJ
authors.lastname: Letesson
authors.forename: N
authors.initials: N
authors.lastname: Lostrie-Trussart
authors.forename: M
authors.initials: M
authors.lastname: Mammerickx
authors.forename: D
authors.initials: D
authors.lastname: Portetelle
authors.forename: A
authors.initials: A
authors.lastname: Burny
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology* / Q000382:microbiology; D002417:Cattle / Q000276:immunology*; D002418:Cattle Diseases / Q000276:immunology* / Q000473:pathology; D003429:Cross Reactions; D005260:Female; D007938:Leukemia / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D001909:Leukemia Virus, Bovine / Q000276:immunology; D012190:Retroviridae / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000951:Antigens, Neoplasm; D000954:Antigens, Surface