Cloned natural suppressor cell lines express the CD3+CD4-CD8- surface phenotype and the alpha, beta heterodimer of the T cell antigen receptor.
Several cloned lines of natural suppressor (NS) cells were studied for their expression of the TCR complex. Almost all bore the CD3+CD4-CD8- surface phenotype with the alpha, beta TCR as judged by immunofluorescent staining. Immunoprecipitation experiments showed a spot on two-dimensional gels which is characteristic of the TCR heterodimer, but neither gamma- nor delta-chains could be precipitated with the appropriate antibodies. NS cells were stimulated to proliferate in vitro with anti-CD3 antibodies and PMA in the presence of irradiated spleen cells. However, anti-CD3 antibodies did not inhibit the suppressive activity of the NS cells. The role of the TCR complex in the suppressive function of these cells remains to be elucidated.
Strober
S
S
Department of Medicine, Stanford University School of Medicine, CA 94305.
Dejbachsh-Jones
S
S
Van Vlasselaer
P
P
Duwe
G
G
Salimi
S
S
Allison
J P
JP
D000818:Animals; D000831:Animals, Newborn / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis* / Q000502:physiology; D002460:Cell Line; D002999:Clone Cells / Q000145:classification* / Q000276:immunology / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D007113:Immunity, Innate; D007958:Leukocyte Count; D015182:Lymphatic Irradiation; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010641:Phenotype; D011233:Precipitin Tests; D011948:Receptors, Antigen, T-Cell / Q000032:analysis* / Q000502:physiology; D050378:T-Lymphocytes, Regulatory / Q000145:classification* / Q000276:immunology / Q000378:metabolism
D000945:Antigens, Differentiation, T-Lymphocyte; D011948:Receptors, Antigen, T-Cell