Influence of methionine supplementation in chelation of lead in rats.
The influence of methionine supplementation on the efficacy of common antidotes to lead poisoning, calcium disodium ethylenediaminetetraacetate (CaNa2EDTA) and D-penicillamine (DPA), was investigated in rats. The animals were given lead acetate (0.1% in drinking water) for 12 weeks and thereafter treated with CaNa2EDTA. DPA (0.3 mmol/kg, intraperitoneally), DL-methionine (1.34 mmol/kg, intragastrically), or the combination of a chelating agent and methionine for 3 days. While chelating agents enhanced the urinary excretion of Pb, methionine increased the fecal excretion of Pb significantly. Treatment with the combination of a chelating agent and methionine did not potentiate the effect of each antidote. However, methionine supplementation increased the efficacy of both chelating agents in reducing the hepatic and renal Pb burden but not the blood Pb level. The Pb-induced inhibition of blood delta-aminolevulinic acid dehydratase activity and the increase in urinary excretion of delta-aminolevulinic acid were reversed to a certain extent by CaNa2EDTA, DPA, and methionine but the combination did not improve their individual performances. The beneficial effects of methionine may be attributed to its ability to increase the bioavailability of glutathione (GSH), useful in chelating Pb and counter-acting the toxic effects, as evidenced by restoration of the Pb-induced decrease in hepatic GSH level by treatment with methionine. Methionine may be useful as a supportive therapy in chelation of Pb.
Kachru
D N
DN
Industrial Toxicology Research Centre, Lucknow, India.
Khandelwal
S
S
Tandon
S K
SK
D000622:Aminolevulinic Acid / Q000652:urine; D000818:Animals; D002614:Chelating Agents / Q000627:therapeutic use; D004359:Drug Therapy, Combination; D004492:Edetic Acid / Q000008:administration & dosage / Q000627:therapeutic use*; D005243:Feces / Q000032:analysis; D005978:Glutathione / Q000378:metabolism; D007854:Lead / Q000378:metabolism / Q000652:urine; D007855:Lead Poisoning / Q000188:drug therapy*; D008297:Male; D008715:Methionine / Q000008:administration & dosage / Q000627:therapeutic use*; D010396:Penicillamine / Q000008:administration & dosage / Q000627:therapeutic use*; D000623:Porphobilinogen Synthase / Q000037:antagonists & inhibitors / Q000097:blood; D011524:Protoporphyrins / Q000097:blood; D051381:Rats
D002614:Chelating Agents; D011524:Protoporphyrins; C017803:zinc protoporphyrin; D007854:Lead; D000622:Aminolevulinic Acid; D004492:Edetic Acid; D008715:Methionine; D000623:Porphobilinogen Synthase; D005978:Glutathione; D010396:Penicillamine