An immunocytochemical study of the proliferating cell nuclear matrix antigen p125/6.5 during rat spermatogenesis.
In previous studies of proliferating mammalian cells a p125/6.5 nuclear matrix antigen displaying a marked increase in mitotic cells has been identified. This antigen was investigated by immunocytochemistry of cryosections of testes at different stages of postnatal development: newborn, 20 days after birth and sexually mature rats. In Sertoli cells, the distribution of the p125/6.5 antigen parallels [3H]thymidine incorporation: present in newborn and absent in sexually mature testes. The p125/6.5 antigen is present also in some prespermatogonia of the newborn rat testis, which do not incorporate [3H]thymidine. At later stages of development, the p125/6.5 antigen is present also in first meiotic prophase spermatocytes displaying an extrachromosomal nucleoplasmic distribution, while absent in spermatids and spermatozoa. These results show that the p125/6.5 antigen increases not only during mitosis, but also during meiosis. They suggest further that this antigen is characteristic of both proliferating cells and cells (prespermatogonia) committed to proliferation.
Hadjiolova
K V
KV
Department of Molecular Genetics, Bulgarian Academy of Sciences, Sofia.
Martinova
Y S
YS
Yankulov
K Y
KY
Davidov
V
V
Kancheva
L S
LS
Hadjiolov
A A
AA
D000818:Animals; D034961:Antigens, Nuclear; D001345:Autoradiography; D004261:DNA Replication; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D008297:Male; D008938:Mitosis; D015530:Nuclear Matrix / Q000648:ultrastructure; D009687:Nuclear Proteins / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D012741:Sexual Maturation; D013091:Spermatogenesis; D013737:Testis / Q000166:cytology / Q000254:growth & development; D013861:Thiocyanates; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D034961:Antigens, Nuclear; D005452:Fluoresceins; D005456:Fluorescent Dyes; D009687:Nuclear Proteins; D013861:Thiocyanates; D014316:Tritium; D016650:Fluorescein-5-isothiocyanate; D013936:Thymidine