title: Soluble CD4 blocks the infectivity of diverse strains of HIV and SIV for T cells and monocytes but not for brain and muscle cells.
abstract: The CD4 antigen has been subverted as a receptor by the human and simian immunodeficiency viruses (HIV-1, HIV-2 and SIV). Several groups have reported that recombinant, soluble forms of the CD4 molecule (sCD4) block the infection of T lymphocytes by HIV-1, as CD4 binds the HIV envelope glycoprotein, gp120, with high affinity. We now report that sCD4 blocks diverse strains of HIV-1, HIV-2 and SIV, but is less effective for HIV-2. The blocking effect is apparent even after adsorption of virions to CD4 cells. Soluble CD4 prevents HIV infection of T-lymphocytic and myelomonocytic cell lines, but neither sCD4 nor anti-CD4 antibodies inhibit infection of glioma and rhabdomyosarcoma cell lines.
authors.affiliation: Chester Beatty Laboratories, Institute of Cancer Research, London, UK.
authors.forename: P R
authors.initials: PR
authors.lastname: Clapham
authors.forename: J N
authors.initials: JN
authors.lastname: Weber
authors.forename: D
authors.initials: D
authors.lastname: Whitby
authors.forename: K
authors.initials: K
authors.lastname: McIntosh
authors.forename: A G
authors.initials: AG
authors.lastname: Dalgleish
authors.forename: P J
authors.initials: PJ
authors.lastname: Maddon
authors.forename: K C
authors.initials: KC
authors.lastname: Deen
authors.forename: R W
authors.initials: RW
authors.lastname: Sweet
authors.forename: R A
authors.initials: RA
authors.lastname: Weiss
mesh_terms: D000954:Antigens, Surface / Q000276:immunology*; D001921:Brain / Q000276:immunology* / Q000382:microbiology; D002460:Cell Line; D015497:HIV-1 / Q000276:immunology* / Q000502:physiology; D015498:HIV-2 / Q000276:immunology* / Q000502:physiology; D006801:Humans; D009000:Monocytes / Q000276:immunology* / Q000382:microbiology; D009132:Muscles / Q000276:immunology* / Q000382:microbiology; D015728:Receptors, HIV; D011991:Receptors, Virus / Q000276:immunology*; D015301:Retroviruses, Simian / Q000276:immunology* / Q000502:physiology; D013601:T-Lymphocytes / Q000276:immunology* / Q000382:microbiology; D010948:Viral Plaque Assay; D014779:Virus Replication
chemical_list: D000954:Antigens, Surface; D015728:Receptors, HIV; D011991:Receptors, Virus