Metabolism of angiotensin peptides by neuronal and glial cultures from rat brain.
The degradation pattern and rate of [Ile5]-Angiotensin (Ang) I, II, and III were studied in neuron-enriched and glia-enriched cells in primary cultures from rat brain. Metabolites were separated by HPLC, and their identities were evaluated by comparison of their retention times with those of synthetic Ang peptide fragments and by analysis of their amino acid composition. Major metabolites were identified as des-Asp1-[Ile5]-Ang I, des-Asp1-[Ile5]-Ang II, [Ile5]-Ang II (3-8) hexapeptide, [Ile5]-Ang II (4-8) pentapeptide, and [Ile5]-Ang II (5-8) tetrapeptide. Glia-enriched cells degraded [Ile5]-Ang I and [Ile5]-Ang III significantly faster than neuron-enriched cells, whereas no difference between the two types of cells was found in the degradation rate of [Ile5]-Ang II. Although the half-lives of [Ile5]-Ang I and [Ile5]-Ang III in neuron-enriched cells from normotensive Wistar-Kyoto (WKY) rats and spontaneously hypertensive rats (SHR) were not significantly different, neuron-enriched cultures from WKY rats metabolized [Ile5]-Ang II about 2.6 times faster than neuron-enriched cells derived from SHR.
Hermann
K
K
Department of Physiology, College of Medicine, University of Florida, Gainesville 32610.
Phillips
M I
MI
Raizada
M K
MK
D000803:Angiotensin I / Q000031:analogs & derivatives / Q000378:metabolism; D000804:Angiotensin II / Q000031:analogs & derivatives / Q000378:metabolism; D000805:Angiotensin III / Q000031:analogs & derivatives / Q000378:metabolism; D000809:Angiotensins / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D006973:Hypertension / Q000378:metabolism*; D007700:Kinetics; D009457:Neuroglia / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
D000809:Angiotensins; D010446:Peptide Fragments; D000804:Angiotensin II; D000805:Angiotensin III; D000803:Angiotensin I; C046239:angiotensin I, Ile(5)-; D007703:Peptidyl-Dipeptidase A; C022912:angiotensin III, 5-Ile-