Micro-quantity tissue digestion for metal measurements by use of a microwave acid-digestion bomb.
We describe a simple and convenient method for processing small amounts of tissue samples for trace-metal measurements by atomic absorption spectrometry, by use of a modified Parr microwave digestion bomb. Digestion proceeds rapidly (less than or equal to 90 s) in a sealed Teflon-lined vessel that eliminates contamination or loss from volatilization. Small quantities of tissue (5-100 mg dry weight) are digested in high-purity nitric acid, yielding concentrations of analyte that can be measured directly without further sample manipulation. We analyzed National Institute of Standards and Technology bovine liver Standard Reference Material to verify the accuracy of the technique. We assessed the applicability of the technique to analysis for aluminum in bone by comparison with a dry ashing procedure.
Nicholson
J R
JR
Department of Pathology, University of Virginia Health Sciences Center, Charlottesville 22908.
Savory
M G
MG
Savory
J
J
Wills
M R
MR
D000535:Aluminum / Q000032:analysis; D001842:Bone and Bones / Q000032:analysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000032:analysis; D008832:Microchemistry; D008872:Microwaves; D009566:Nitrates; D017942:Nitric Acid; D013054:Spectrophotometry, Atomic; D014131:Trace Elements / Q000032:analysis*
D009566:Nitrates; D014131:Trace Elements; D017942:Nitric Acid; D000535:Aluminum