title: Convenient uses of polymerase chain reaction in analyzing recombinant cDNA clones.
abstract: We have used polymerase chain reaction to accelerate our analysis of recombinant lambda-cDNA clones. We have amplified the inserts of lambda gt10 or lambda gt11 recombinants starting with bacteriophage in cored plaques or isolated DNA. The amplifications made with simple or complex oligonucleotide primers have allowed convenient sizing, subcloning and translation of the phage inserts into protein.
authors.affiliation: Dept. of Biology, University of North Carolina, Chapel Hill 27599-3280.
authors.forename: B K
authors.initials: BK
authors.lastname: Nishikawa
authors.forename: D M
authors.initials: DM
authors.lastname: Fowlkes
authors.forename: B K
authors.initials: BK
authors.lastname: Kay
mesh_terms: D000595:Amino Acid Sequence; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D001709:Biotechnology; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000235:genetics; D004274:DNA, Recombinant; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction / Q000379:methods*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D004274:DNA, Recombinant; D014764:Viral Proteins; D004247:DNA