Expression of the fusion glycoprotein of human parainfluenza type 3 virus in insect cells by a recombinant baculovirus and analysis of its immunogenic property.
The fusion (F) glycoprotein of human parainfluenza type 3 (PI3) virus was produced in insect cells using a baculovirus expression vector (pAcYM1). The recombinant glycoprotein was identified by its reactivity with specific monoclonal and polyclonal antibodies and showed an apparent molecular mass of 70 kDa. Although the fusion protein was found on the infected cell surface, it did not appear to be proteolytically cleaved to F1 and F2 subunits. Immunization of hamsters with the recombinant protein elicited antibody which neutralized infectivity and blocked fusion of virus-infected cells. The protective response to challenge infection of immunized hamsters was similar to that observed with affinity purified F from PI3 virus (Ray et al., J. Virol. 62, 783-787, 1988).
Ray
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Molecular Engineering Associates, Inc., Birmingham, AL.
Galinski
M S
MS
Compans
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RW
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015139:Blotting, Southern; D002460:Cell Line; D006224:Cricetinae; D005822:Genetic Vectors; D006023:Glycoproteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007304:Insect Viruses / Q000235:genetics; D010224:Parainfluenza Virus 3, Human / Q000235:genetics*; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D018108:Respirovirus / Q000235:genetics*; D014162:Transfection; D014614:Vaccines, Synthetic; D014709:Vero Cells; D014760:Viral Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines
D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D007106:Immune Sera; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D014760:Viral Fusion Proteins; D014765:Viral Vaccines