Organization and orientation of a human T cell receptor delta chain V gene segment that suggests an inversion mechanism is utilized in its rearrangement.
A human T cell receptor (TcR) delta chain variable region segment, V delta 3, has been mapped 2 kilobases 3' to the constant (C delta) gene. The V delta 3 gene segment is in an inverted transcriptional orientation to the diversity regions (D delta), joining regions (J delta) and C delta gene segments. Analyses of TcR delta cDNA clone, KT041, showed that V delta 3 is linked to D delta 1, D delta 2, J delta 3 and C delta and that this message encodes a potentially functional TcR delta chain. These findings indicate that a functional TcR delta gene can be constructed through a chromosomal inversion, suggesting that in human, chromosomal inversion is one of the important rearrangement mechanisms.
Takihara
Y
Y
Department of Medical Biophysics and Immunology, University of Toronto, Canada.
Champagne
E
E
Ciccone
E
E
Moretta
L
L
Minden
M
M
Mak
T W
TW
D001483:Base Sequence; D007446:Chromosome Inversion; D004247:DNA / Q000032:analysis; D015329:Gene Rearrangement, T-Lymphocyte; D006801:Humans; D008969:Molecular Sequence Data; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D016692:Receptors, Antigen, T-Cell, gamma-delta
D011948:Receptors, Antigen, T-Cell; D016692:Receptors, Antigen, T-Cell, gamma-delta; D004247:DNA