Carbonic anhydrase III and collagen markers in serum following cross-country skiing.
Protein leakage from type I skeletal muscle cells and collagen synthesizing cells were studied during a 24-h cross-country skiing relay in eight men using serum carbonic anhydrase III (S-CA III) and serum galactosylhydroxylysyl glucosyltransferase (S-GGT) as the respective specific marker proteins. Serum aminoterminal propeptide of type III procollagen (S-Pro(III)-N-P) was used as an indicator of type III collagen synthesis and serum creatine kinase (S-CK) as a nonspecific muscular marker. S-CK, S-CA III, and S-GGT were increased immediately following the skiing relay by 870%, 640%, and 40%, respectively. No significant change was observed in the concentration of S-Pro(III)-N-P following the exercise. A high positive correlation (rs = 0.92, P less than 0.01) was found between the increase in S-CA III and S-CK, whereas no correlation was observed between these muscular markers and S-GGT. The results suggest leakage from type I skeletal muscle cells and collagen synthesizing cells as a consequence of prolonged cross-country skiing, but there seems to exist no dependence between acute responses in muscular and connective tissue cells to exercise.
Takala
T E
TE
Department of Sports Medicine, University of Oulu, Finland.
Vuori
J J
JJ
Rahkila
P J
PJ
Hakala
E O
EO
Karpakka
J A
JA
Alen
M J
MJ
Orava
Y S
YS
Väänänen
H K
HK
D000328:Adult; D002256:Carbonic Anhydrases / Q000097:blood*; D002986:Clinical Trials as Topic; D003094:Collagen / Q000096:biosynthesis / Q000097:blood*; D003402:Creatine Kinase / Q000097:blood; D005964:Glucosyltransferases / Q000097:blood; D006801:Humans; D008297:Male; D009132:Muscles / Q000201:enzymology*; D010446:Peptide Fragments / Q000097:blood; D011347:Procollagen / Q000097:blood; D012865:Skiing
D010446:Peptide Fragments; D011347:Procollagen; C041876:procollagen Type III-N-terminal peptide; D003094:Collagen; D005964:Glucosyltransferases; C023247:UDP glucose-collagen glucosyltransferase; D003402:Creatine Kinase; D002256:Carbonic Anhydrases