title: Interactions of Pseudorabies virus with swine alveolar macrophages I: virus replication.
abstract: The replication of Pseudorabies virus (PRV) in cultured swine alveolar macrophages (AM) was studied using 6 different virus strains. AM were highly permissive to PRV infection, with progeny virus titres of 10(7) TCID50/ml from some strains. Virus progeny titres were higher in cultures infected with the field strains S-62 and 4892 than in cultures infected with the strains Bartha or PRV-C. Virus replication, viral DNA synthesis and the concomitant cell damage were dependent upon virus input m.o.i.s. and virus strain. Furthermore, cells from 7 day old pigs yielded higher virus progeny titres than cells of 6 week old pigs. The results from this study provide support to the premise that PRV infection may predispose anials to respiratory disease.
authors.affiliation: Department of Large Animal Clinical Sciences, College of Veterinary Medicine University of Minnesota, St. Paul.
authors.forename: G
authors.initials: G
authors.lastname: Iglesias
authors.forename: C
authors.initials: C
authors.lastname: Pijoan
authors.forename: T
authors.initials: T
authors.lastname: Molitor
mesh_terms: D000375:Aging / Q000276:immunology; D000818:Animals; D002478:Cells, Cultured; D004279:DNA, Viral / Q000096:biosynthesis; D011558:Herpesvirus 1, Suid / Q000502:physiology*; D008264:Macrophages / Q000382:microbiology*; D011650:Pulmonary Alveoli / Q000276:immunology / Q000382:microbiology; D013045:Species Specificity; D013552:Swine; D014779:Virus Replication
chemical_list: D004279:DNA, Viral