Molecular size of the gamma-aminobutyric acidA receptor purified from mammalian cerebral cortex.
The hydrodynamic behaviour of both the soluble and purified gamma-aminobutyric acidA (GABAA) receptor of bovine or rat cerebral cortex has been investigated in solution in Triton X-100 or in 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulphonate (CHAPS). In all the hydrodynamic separations made, it was found that the binding activities for GABA, benzodiazepine, and (where detectable) t-butylbicyclophosphorothionate comigrated. Conditions were established for gel exclusion chromatography and for sucrose density gradient velocity sedimentation that maintain the GABAA receptor in a nonaggregated form. Using these conditions, the molecular weight of the bovine GABAA receptor in the above-mentioned detergents was calculated using the H2O/2H2O method. A value of Mr 230,000-240,000 was calculated for the bovine pure GABAA receptor purified in sodium deoxycholate/Triton X-100 media. A value of Mr 284,000-290,000 was calculated for the nonaggregated bovine or rat cortex receptor in CHAPS, but the Stokes radius is smaller in the latter than in the former medium and the detergent binding in CHAPS is underestimated. Thus the deduced Mr, 240,000, is the best estimate by this method.
Mamalaki
C
C
MRC Molecular Neurobiology Unit, Medical Research Council Centre, Cambridge, England.
Barnard
E A
EA
Stephenson
F A
FA
D000818:Animals; D002417:Cattle; D002499:Centrifugation, Density Gradient; D002540:Cerebral Cortex / Q000032:analysis*; D002793:Cholic Acids; D002850:Chromatography, Gel; D008433:Mathematics; D008970:Molecular Weight; D017830:Octoxynol; D011092:Polyethylene Glycols; D011963:Receptors, GABA-A / Q000302:isolation & purification*
D002793:Cholic Acids; D011963:Receptors, GABA-A; D011092:Polyethylene Glycols; D017830:Octoxynol; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate