[Studies of enzyme histochemistry and ultrastructure of the myocardium in rats with streptozotocin-induced diabetes].
Experimental diabetes was induced in wistar rats by intraperitoneal streptozotocin in a single dose of 60-65 mg per kg body weight. The changes of myocardium enzyme histochemistry and ultrastructure were observed during the 2nd, 4th, 6th, 12th week of diabetic state. The glucose metabolism enzyme activities such as ICDH, SDH, MDH, LDH, all decreased. The results indicated that glucose oxidation and glycolysis reduced. In the ventricular myocardium of diabetic rats, varying degrees of ultrastructural change were apparent. Swelling of mitochondria was observed. Focal areas showed myofibrillar degeneration, and cardiac muscle muscle cells showed condensation of nuclear chromatin. Lipid droplets could be seen in the cytoplasm of cardiac myocytes. The ultrastructural changes in the cardiac muscle cells were not accompanied by any changes in the endothelial cells and smooth muscle cells of the small vessels or capillaries. This study provides a strong evidence for the occurrence of a primary myocardial disease in the model of streptozotocin-induced diabetes. The primary cardiomyopathy was not dependent on vascular pathological changes.
Cai
F
F
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology* / Q000473:pathology; D006651:Histocytochemistry; D008297:Male; D009206:Myocardium / Q000201:enzymology* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D013311:Streptozocin
D013311:Streptozocin