Identification of Mycoplasma binding proteins utilizing a 100 kilodalton lung fibroblast receptor.
A 100 kilodalton glycoprotein receptor for Mycoplasma pneumoniae has been isolated from MRC-5 human lung fibroblasts. This receptor, as well as anti-receptor serum, were both capable of inhibiting the attachment of 14C-labelled M. pneumoniae to MRC-5 fibroblasts. The receptor was also capable of inhibiting the attachment of C-labelled M. gallisepticum and M. genitalium, but not M. pulmonis, to MRC-5 fibroblasts. This indicates that a common sequence may exist in these binding proteins of M. pneumoniae, M. genitalium, and M. gallisepticum. This receptor and anti-receptor serum were utilized to probe M. pneumoniae, M. genitalium, and M. gallisepticum for their corresponding binding proteins. A 32 kilodalton protein in M. pneumoniae, a 90 kilodalton protein in M. genitalium and a 139 kilodalton protein in M. gallisepticum were recognized.
Geary
S J
SJ
Bionique Laboratories, Inc., Saranac Lake, New York 12983.
Gabridge
M G
MG
Intres
R
R
Draper
D L
DL
Gladd
M F
MF
D001422:Bacterial Adhesion / Q000502:physiology*; D001426:Bacterial Proteins / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000032:analysis / Q000382:microbiology; D006801:Humans; D015151:Immunoblotting; D008168:Lung / Q000032:analysis / Q000166:cytology / Q000382:microbiology*; D008562:Membrane Glycoproteins / Q000302:isolation & purification*; D008970:Molecular Weight; D009177:Mycoplasma pneumoniae / Q000378:metabolism*
D001426:Bacterial Proteins; D002352:Carrier Proteins; D008562:Membrane Glycoproteins