Liquid chromatographic-atmospheric pressure ionization mass spectrometric analysis of toremifene metabolites in human urine.
A liquid chromatographic-atmospheric pressure ionization mass spectrometric method has been developed for the analysis of toremifene metabolites in human urine after oral administration. This ionization source is a useful device for studying metabolites of toremifene because the total effluent from high-performance liquid chromatography is fed through an interface with a direct heating nebulizer and vaporizer at atmospheric pressure. To obtain good sensitivity the use of the right mobile phase is very important: ammonium acetate in methanol in the case of toremifene and its metabolites. Four unconjugated and three glucuronide-conjugated metabolites were detected in human urine. The majority of these were new and distinguishable from known metabolites.
Watanabe
N
N
Research Laboratories, Pharmaceuticals Group, Nippon Kayaku Co., Ltd., Tokyo, Japan.
Irie
T
T
Koyama
M
M
Tominaga
T
T
D000970:Antineoplastic Agents / Q000378:metabolism* / Q000652:urine; D001274:Atmospheric Pressure; D001943:Breast Neoplasms / Q000652:urine; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry; D013056:Spectrophotometry, Ultraviolet; D013629:Tamoxifen / Q000031:analogs & derivatives* / Q000378:metabolism / Q000652:urine; D017312:Toremifene
D000970:Antineoplastic Agents; D013629:Tamoxifen; D017312:Toremifene