DNA fragments which specifically bind to isolated nuclear matrix in vitro interact with matrix-associated DNA topoisomerase II.
A matrix-associated region (MAR)-containing fragment has been selected from the library of cloned chicken nuclear matrix-associated DNA fragments. Factors, which determine the specific binding of DNA fragments have been studied. Using topoisomerase II-specific inhibitor VM 26 we established that nuclear matrix-associated topoisomerase II interacted with the MAR-containing DNA fragment producing specific cleavage sites on DNA of the fragment.
Vassetzky
Y S
YS
Institute of Molecular Biology, USSR Academy of Sciences, Moscow.
Razin
S V
SV
Georgiev
G P
GP
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D002478:Cells, Cultured; D002645:Chickens; D004250:DNA Topoisomerases, Type II / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific; D004900:Erythroblasts / Q000378:metabolism; D051379:Mice
D004269:DNA, Bacterial; D015252:Deoxyribonucleases, Type II Site-Specific; D004250:DNA Topoisomerases, Type II