Cloning and sequencing of a phospholipase C gene of Clostridium perfringens.
The gene encoding phospholipase C (alpha-toxin) of Clostridium perfringens was cloned into lambda gt10. The maximal size of the coding region was 1.4 kb and the minimum was 1.1 kb as determined by subcloning into the vector pBR322 and testing for activity. The nucleotide sequence of this region contained a single open reading frame of 1194 bp corresponding to a protein of Mr 45473 with a possible N-terminal signal sequence of 28 amino acids which when removed, would give a mature protein of Mr 42521. This is in good agreement with the reported size of 43 kDa. The coding region has a dG + dC content of 33.7%, and the codon usage displays a pronounced preference for codons with the lowest dG + dC content.
Okabe
A
A
Department of Microbiology, Kagawa Medical School, Japan.
Shimizu
T
T
Hayashi
H
H
D000595:Amino Acid Sequence; D010582:Bacteriophage lambda / Q000235:genetics; D001482:Base Composition; D001483:Base Sequence; D003001:Cloning, Molecular; D003016:Clostridium perfringens / Q000201:enzymology* / Q000235:genetics; D003062:Codon; D004262:DNA Restriction Enzymes; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012689:Sequence Homology, Nucleic Acid; D013753:Tetracycline Resistance / Q000235:genetics; D014158:Transcription, Genetic; D010738:Type C Phospholipases / Q000235:genetics*
D003062:Codon; D004262:DNA Restriction Enzymes; D010738:Type C Phospholipases