Synthesis and characterization of fluorescein- and rhodamine-labeled probes for vasotocin receptors.
Fluorescent analogues of vasotocin, [1-(beta-mercaptopropionic acid), 9-(p-aminofluoresceinylphenylalanine)]arginine vasotocin [[MPA1, (p-NH2flu)Phe9]AVT] and [1-(beta'-mercaptopropionic acid), 9-(p-amino rhodaminylphenylalanine)]AVT [[MPA1, (p-NH2rhod)-Phe9]AVT], were synthesized by the solid-phase method. These compounds yielded half-maximal hydrosmotic responses (half-maximal values) in the toad urinary bladder at 2 X 10(-9) M. Epifluorescence microscopy showed punctate basal localization of analogues on the majority of bladder epithelial cells within 20 min. The cellular localization was prevented by vasotocin. These fluorescent analogues may serve as useful probes for vasotocin receptors in toad bladder and in tissues from other species that use vasotocin as their antidiuretic-pressor hormone.
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Department of Physiology and Biophysics, Mount Sinai Medical School, City University of New York, New York 10029.
Masur
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Eggena
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D000595:Amino Acid Sequence; D000818:Animals; D002024:Bufo marinus; D005260:Female; D005452:Fluoresceins; D005456:Fluorescent Dyes; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D011945:Receptors, Angiotensin / Q000378:metabolism*; D017483:Receptors, Vasopressin; D012235:Rhodamines; D001743:Urinary Bladder / Q000378:metabolism; D014668:Vasotocin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000378:metabolism*
D005452:Fluoresceins; D005456:Fluorescent Dyes; D011945:Receptors, Angiotensin; D017483:Receptors, Vasopressin; D012235:Rhodamines; C034862:vasotocin receptor; C062297:vasotocin, 1-(beta-mercaptopropionic acid)-8-Arg-9-(4-aminofluoresceinyl-Phe)-; C062298:vasotocin, 1-(beta-mercaptopropanoic acid)-8-Arg-9-(4-aminorhodaminyl-Phe)-; D014668:Vasotocin