A 32 kDa lipocortin from human mononuclear cells appears to be identical with the placental inhibitor of blood coagulation.
A 32 kDa protein isolated from human mononuclear cells is a member of the lipocortin family, a new group of Ca2+-dependent lipid-binding proteins thought to be involved in the regulation of phospholipase A2, in exocytosis and in membrane-cytoskeleton interactions. Purification of this protein was based on its ability to associate with membrane phospholipids in a Ca2+-dependent manner and its capacity to inhibit purified phospholipase A2 from pig pancreas. Using immunological detection, we show that it is present in various cells involved in the inflammatory and coagulation processes. We present extensive amino acid data that strongly suggest that this protein is identical with a recently described inhibitor of blood coagulation, with endonexin II and with lipocortin V. Sequence alignment with other known proteins show a significant degree of homology with lipocortins I and II, the substrates of the epidermal-growth-factor receptor tyrosine kinase and the oncogene pp60src tyrosine kinase respectively, and with protein II. The possible physiological role of this 32 kDa lipocortin is discussed.
Rothhut
B
B
I.N.S.E.R.M. U.A. 285, Institut Pasteur, Paris, France.
Coméra
C
C
Cortial
S
S
Haumont
P Y
PY
Diep Le
K H
KH
Cavadore
J C
JC
Conard
J
J
Russo-Marie
F
F
Lederer
F
F
D000595:Amino Acid Sequence; D017304:Annexin A5; D000925:Anticoagulants; D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000097:blood / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000032:analysis*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010743:Phospholipids / Q000378:metabolism
D017304:Annexin A5; D000925:Anticoagulants; D002135:Calcium-Binding Proteins; D010743:Phospholipids