title: Receptor-mediated phagocytosis in human neutrophils is associated with increased formation of inositol phosphates and diacylglycerol. Elevation in cytosolic free calcium and formation of inositol phosphates can be dissociated from accumulation of diacylglycerol.
abstract: Phagocytosis of C3bi- or IgG-opsonized yeast particles in human neutrophils was found to be associated with an increased formation of inositol phosphates and diacylglycerol. Pertussis toxin only marginally affected phagocytosis of IgG- and C3bi-opsonized particles and the associated formation of second messengers. Forskolin, which induced a threefold rise of cellular cAMP, however, markedly inhibited both C3bi- and IgG-mediated phagocytosis as well as the particle-induced formation of inositol phosphates and diacylglycerol. These observations are in contrast to what was found to occur with chemotactic factors and indicate that chemotactic and phagocytic signaling can be regulated independently in human neutrophils. Since C3bi-mediated phagocytosis has been shown to occur at vanishingly low cytosolic free calcium levels, calcium-depleted cells were used to study the importance of the inositol cycle for the engulfment of C3bi-opsonized particles. Despite a total lack of receptor-induced formation of inositol phosphates, a significantly increased accumulation of diacylglycerol accompanied the ingestion of C3bi-opsonized particles. These data show that the engulfment of C3bi-opsonized particles can occur independently of both a calcium transient and an increased inositol phosphate production. However, the observed accumulation of diacylglycerol, not derived from phosphoinositides, suggests that this second messenger play a role in the control of the engulfment process.
authors.affiliation: Department of Medical Microbiology, University of Linköping, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Fällman
authors.forename: D P
authors.initials: DP
authors.lastname: Lew
authors.forename: O
authors.initials: O
authors.lastname: Stendahl
authors.forename: T
authors.initials: T
authors.lastname: Andersson
mesh_terms: D000943:Antigens, Differentiation / Q000378:metabolism / Q000502:physiology*; D002118:Calcium / Q000378:metabolism*; D005576:Colforsin / Q000494:pharmacology; D003179:Complement C3b / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004075:Diglycerides / Q000096:biosynthesis* / Q000378:metabolism; D005989:Glycerides / Q000096:biosynthesis*; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D007295:Inositol Phosphates / Q000096:biosynthesis* / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D009895:Opsonin Proteins; D037342:Pertussis Toxin; D010587:Phagocytosis / Q000187:drug effects; D011951:Receptors, Complement / Q000187:drug effects / Q000502:physiology*; D017463:Receptors, Complement 3b; D011961:Receptors, Fc / Q000378:metabolism / Q000502:physiology*; D017452:Receptors, IgG; D013403:Sugar Phosphates / Q000096:biosynthesis*; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
chemical_list: D000943:Antigens, Differentiation; D004075:Diglycerides; D005989:Glycerides; D007074:Immunoglobulin G; D007295:Inositol Phosphates; D009895:Opsonin Proteins; D011951:Receptors, Complement; D017463:Receptors, Complement 3b; D011961:Receptors, Fc; D017452:Receptors, IgG; D013403:Sugar Phosphates; D010566:Virulence Factors, Bordetella; D005576:Colforsin; D003179:Complement C3b; D037342:Pertussis Toxin; D002118:Calcium