title: The amino acid sequence of cytochrome c553 from Microcystis aeruginosa.
abstract: Cytochrome c553 is an electron donor to P700 in the photosynthetic electron transfer chain of cyanobacteria and eukaryotic algae. We have purified this cytochrome from the cyanobacterium Microcystis aeruginosa and determined its amino acid sequence. When the amino acid sequence of this protein is compared to sequences of cytochromes c553 from other organisms, one sees that the evolution of net charge is more pronounced than the evolution of overall structure, further documenting a pronounced shift in the isoelectric point of this protein during the evolution of cyanobacteria. Cyanobacteria and algae also contain cytochrome c550 (Mr 15,500) which is quite different from cytochrome c553 (Mr 10,500). When the amino acid sequence of cytochrome c553 is compared to that of cytochrome c550, two regions of similar sequence are recognized.
authors.affiliation: Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907.
authors.forename: C L
authors.initials: CL
authors.lastname: Cohn
authors.forename: M A
authors.initials: MA
authors.lastname: Hermodson
authors.forename: D W
authors.initials: DW
authors.lastname: Krogmann
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D003574:Cytochrome c Group / Q000235:genetics / Q000302:isolation & purification*; D006868:Hydrolysis; D046931:Microcystis / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010446:Peptide Fragments / Q000302:isolation & purification
chemical_list: D000596:Amino Acids; D003574:Cytochrome c Group; D010446:Peptide Fragments; C083951:cytochrome c553; C022302:cytochrome C-552; C031732:cytochrome C-550