A syncytia assay for human immunodeficiency virus type I (HIV-I) envelope protein and its use in studying HIV-I mutations.
We describe a syncytia assay that utilizes a noninfectious plasmid (pEVd1443) derived from human immunodeficiency virus type I (HIV-I). This plasmid carries a large deletion of gag-pol sequences but expresses HIV-I envelope proteins and induces syncytia following transfection into HeLa-CD4 cells. This plasmid was used to study the effects of mutations in the gp 120 and gp41 portions of the envelope gene of HIV-I.
Felser
J M
JM
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
Klimkait
T
T
Silver
J
J
D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics; D015153:Blotting, Western; D005786:Gene Expression Regulation; D015683:Gene Products, gag; D015696:Gene Products, tat; D015488:HIV Antigens / Q000235:genetics; D015699:HIV Envelope Protein gp120; D019302:HIV Envelope Protein gp160; D015700:HIV Envelope Protein gp41; D015497:HIV-1 / Q000235:genetics* / Q000276:immunology; D006367:HeLa Cells; D006801:Humans; D009154:Mutation; D010957:Plasmids; D011233:Precipitin Tests; D012191:Retroviridae Proteins / Q000032:analysis* / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D014162:Transfection; D014759:Viral Envelope Proteins / Q000032:analysis* / Q000235:genetics; D054322:tat Gene Products, Human Immunodeficiency Virus
D000945:Antigens, Differentiation, T-Lymphocyte; D015683:Gene Products, gag; D015696:Gene Products, tat; D015488:HIV Antigens; D015699:HIV Envelope Protein gp120; D019302:HIV Envelope Protein gp160; D015700:HIV Envelope Protein gp41; D012191:Retroviridae Proteins; D014157:Transcription Factors; D014759:Viral Envelope Proteins; D054322:tat Gene Products, Human Immunodeficiency Virus