Rapid diagnosis of Aujeszky's disease in pigs by improved in situ hybridization using biotinylated probes on paraffin-embedded tissue sections.
A practical in situ hybridization (ISH) method has been developed for the rapid diagnosis of pseudorabies virus (PRV) infection in pigs. The method utilizes routine formalin fixed and paraffin-embedded tissue sections, non radioactive biotin-labelling, simple hybridization procedure and diaminobenzidine (DAB) silver enhancement detection system. The rapid (ISH) method showed concordance with the direct filter hybridization (DFH) and with the peroxidase-antiperoxidase (PAP) assays. By the simplified ISH, diagnosis can be made within two days.
Belák
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Funa
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Kelly
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Belák
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D000818:Animals; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis*; D011558:Herpesvirus 1, Suid / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D011557:Pseudorabies / Q000175:diagnosis*; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*
D015342:DNA Probes; D004279:DNA, Viral