Translocation of phosphatidate phosphohydrolase from the cytosol to microsomal membranes in thioacetamide-induced liver tumours in rats.
The translocation of phosphatidate phosphohydrolase induced by oleate was higher (two-fold) in liver homogenates obtained from long-term thioacetamide-treated rats than from control rats. These differences between thioacetamide-treated and control livers were noticeably higher (four-fold) in the presence of physiological concentrations of salt (0.15 M KCl). In homogenates from control rats, there was a lack of response when physiological concentrations of the salt were present. The enhanced response to translocate phosphatidate phosphohydrolase activity in liver homogenates from thioacetamide-treated rats was due to an increased binding ability of microsomal membranes.
Cascales
C
C
Instituto de Bioquímica, Facultad de Farmacia, Madrid, Spain.
Boscá
L
L
Martín-Sanz
P
P
Brindley
D N
DN
Cascales
M
M
D000081:Acetamides / Q000633:toxicity*; D000818:Animals; D003600:Cytosol / Q000201:enzymology*; D007425:Intracellular Membranes / Q000201:enzymology*; D008113:Liver Neoplasms / Q000139:chemically induced* / Q000201:enzymology; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D019301:Oleic Acid; D009829:Oleic Acids / Q000494:pharmacology; D010711:Phosphatidate Phosphatase / Q000378:metabolism*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000201:enzymology; D013853:Thioacetamide / Q000633:toxicity*
D000081:Acetamides; D009829:Oleic Acids; D013853:Thioacetamide; D019301:Oleic Acid; D010744:Phosphoric Monoester Hydrolases; D010711:Phosphatidate Phosphatase