Maturation and subcellular compartmentation of potato starch phosphorylase.
The subcellular localization and maturation of starch phosphorylase (EC 2.4.1.1) was studied in developing potato tubers. The enzyme is localized inside the stroma of amyloplasts in young tubers, whereas in mature tubers it is found within the cytoplasm in the immediate vicinity of the plastids. A phosphorylase cDNA clone was isolated and used in RNA gel blot experiments to demonstrate that phosphorylase mRNAs are of the same size and abundance in both young and mature tubers. In vitro translation of mRNAs followed by immunoprecipitation with a phosphorylase antiserum indicates that the enzyme is synthesized as a higher molecular weight precursor in both young and mature tubers. The presence of a transit peptide at the N terminus of the protein was confirmed by the sequencing of the phosphorylase cDNA clone. The transit peptide has several structural features common to transit peptides of chloroplast proteins but contains a surprisingly large number of histidine residues. The mature form of the enzyme is present in both young and mature tubers, suggesting that a similar processing of the transit peptide may take place in two different subcellular locations.
Brisson
N
N
Department of Biochemistry, University of Montreal, Quebec, Canada.
Giroux
H
H
Zollinger
M
M
Camirand
A
A
Simard
C
C
D000595:Amino Acid Sequence; D001483:Base Sequence; D002451:Cell Compartmentation; D004247:DNA; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D006005:Phosphorylases / Q000235:genetics / Q000378:metabolism*; D014176:Protein Biosynthesis; D011198:Solanum tuberosum / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000648:ultrastructure
D004247:DNA; D006005:Phosphorylases