title: Lipolytic activity of porcine adipose tissue after preincubation.
abstract: 1. The stimulated lipolytic rate in porcine adipose tissue slices was markedly increased after preincubation with or without isoproterenol. 2. Preincubation with and without insulin, dibutyryl-cAMP or theophylline suggested that the activation of the stimulated rate resulted from inhibition or inactivation of cAMP-phosphodiesterase activity during preincubation. 3. Preincubation with isoproterenol also caused activation of the basal (no exogenous hormone) lipolytic activity. 4. However, much of this activation appeared to result from carry-over of isoproterenol into the incubation medium because it could be lowered by additional washing of the tissue, by lower isoproterenol concentration or by propranolol.
authors.affiliation: U.S. Department of Agriculture, Clay Center, NE 68933.
authors.forename: H J
authors.initials: HJ
authors.lastname: Mersmann
mesh_terms: D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000037:antagonists & inhibitors; D000241:Adenosine / Q000494:pharmacology; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D005260:Female; D007328:Insulin / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D008066:Lipolysis / Q000187:drug effects; D008297:Male; D011433:Propranolol / Q000494:pharmacology; D013552:Swine; D013806:Theophylline / Q000494:pharmacology
chemical_list: D007328:Insulin; D003994:Bucladesine; D011433:Propranolol; D013806:Theophylline; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D000241:Adenosine; D007545:Isoproterenol