Forskolin infusion in vivo increases ouabain binding in brain.
In order to investigate the possible regulation of brain Na,K-ATPase by cyclic AMP, we measured Na,K-ATPase activity and ouabain binding in cerebral cortex after intraventricular infusion of forskolin for 7 days. There was a dose-related increase in high-affinity ouabain binding, with a 75% increase at 12.5 micrograms/h forskolin. The effect on total enzyme activity was smaller but enzyme activity with high affinity for ouabain was increased by 65%, suggesting a selective effect on enzyme with high affinity for ouabain. Forskolin appeared not to interact directly with Na,K-ATPase in vitro.
Swann
A C
AC
Department of Psychiatry, University of Texas Medical School, Houston 77225.
Steketee
J
J
D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D005576:Colforsin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D007276:Injections, Intraventricular; D008297:Male; D010042:Ouabain / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D005576:Colforsin; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase