The proteins associated with the soluble form of p36, the main target of the src oncogene product in chicken fibroblasts, are glycolytic enzymes.
One of the main targets of pp60v-src tyrosine kinase is a 34 to 39-kilodalton protein of chicken embryo fibroblasts called p36 or calpactin I. We have previously reported an association of the cytoplasmic fraction of p36 (10-20% of the total cellular p36) with three chicken polypeptides named p32, p48, and p54. We have now raised and affinity-purified antibodies against each of these proteins. This has allowed their identification: p32 is lactate dehydrogenase, p48 is enolase, and p54 is phosphoglucose isomerase. An association between p36 and two other known substrates of pp60v-src, the glycolytic enzymes enolase and lactate dehydrogenase, suggests a cellular organization of the various targets of the oncogene tyrosine kinases. Furthermore, a possible relationship between p36 and glycolysis is questioned.
Simon
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University of Geneva, Department of Molecular Biology, Switzerland.
Spahr
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Dainous
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D000818:Animals; D017302:Annexins; D000918:Antibody Specificity; D002135:Calcium-Binding Proteins / Q000032:analysis*; D002642:Chick Embryo; D015180:Electrophoresis, Gel, Two-Dimensional; D005347:Fibroblasts / Q000201:enzymology; D005956:Glucose-6-Phosphate Isomerase / Q000032:analysis*; D015151:Immunoblotting; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D008565:Membrane Proteins / Q000032:analysis*; D015688:Oncogene Protein pp60(v-src) / Q000378:metabolism; D010751:Phosphopyruvate Hydratase / Q000032:analysis*; D011233:Precipitin Tests; D012995:Solubility
D017302:Annexins; D002135:Calcium-Binding Proteins; D008565:Membrane Proteins; D007770:L-Lactate Dehydrogenase; D015688:Oncogene Protein pp60(v-src); D010751:Phosphopyruvate Hydratase; D005956:Glucose-6-Phosphate Isomerase