Sporozoite vaccine induces genetically restricted T cell elimination of malaria from hepatocytes.
The target of the CD8+ T cell-dependent immunity that protects mice immunized with irradiation-attenuated malaria sporozoites has not been established. Immune BALB/c mice were shown to develop malaria-specific, CD8+ T cell-dependent inflammatory infiltrates in their livers after challenge with Plasmodium berghei sporozoites. Spleen cells from immune BALB/c and C57BL/6 mice eliminated hepatocytes infected with the liver stage of P. berghei in vitro. The activity against infected hepatocytes is not inhibited by antibodies to interferon-gamma and is not present in culture supernatants. It is genetically restricted, an indication that malaria antigens on the hepatocyte surface are recognized by immune T effector cells. Subunit vaccine development will require identification of the antigens recognized by these T cells and a method of immunization that induces such immunity.
Hoffman
S L
SL
Infectious Diseases Department, Naval Medical Research Institute, Bethesda, MD 20814.
Isenbarger
D
D
Long
G W
GW
Sedegah
M
M
Szarfman
A
A
Waters
L
L
Hollingdale
M R
MR
van der Meide
P H
PH
Finbloom
D S
DS
Ballou
W R
WR
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000913:Antibodies, Protozoan / Q000032:analysis; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology; D006183:H-2 Antigens / Q000276:immunology; D007114:Immunization; D007371:Interferon-gamma / Q000276:immunology / Q000494:pharmacology; D008099:Liver / Q000276:immunology / Q000469:parasitology*; D008288:Malaria / Q000276:immunology* / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010962:Plasmodium berghei / Q000276:immunology*; D011994:Recombinant Proteins; D013154:Spleen / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D014612:Vaccines / Q000276:immunology
D000906:Antibodies; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D006183:H-2 Antigens; D011994:Recombinant Proteins; D014612:Vaccines; D007371:Interferon-gamma