Selective amplification and cloning of four new members of the G protein-coupled receptor family.
An approach based on the polymerase chain reaction has been devised to clone new members of the family of genes encoding guanosine triphosphate-binding protein (G protein)-coupled receptors. Degenerate primers corresponding to consensus sequences of the third and sixth transmembrane segments of available receptors were used to selectively amplify and clone members of this gene family from thyroid complementary DNA. Clones encoding three known receptors and four new putative receptors were obtained. Sequence comparisons established that the new genes belong to the G protein-coupled receptor family. Close structural similarity was observed between one of the putative receptors and the 5HT1a receptor. Two other molecules displayed common sequence characteristics, suggesting that they are members of a new subfamily of receptors with a very short nonglycosylated (extracellular) amino-terminal extension.
Libert
F
F
Institut de Recherche Interdisciplinaire, Faculté de Médecine, Université Libre de Bruxelles, Campus Erasme, Belgium.
Parmentier
M
M
Lefort
A
A
Dinsart
C
C
Van Sande
J
J
Maenhaut
C
C
Simons
M J
MJ
Dumont
J E
JE
Vassart
G
G
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004259:DNA-Directed DNA Polymerase; D019204:GTP-Binding Proteins / Q000378:metabolism*; D005784:Gene Amplification; D006801:Humans; D008969:Molecular Sequence Data; D011942:Receptors, Adrenergic, alpha / Q000235:genetics; D011943:Receptors, Adrenergic, beta / Q000235:genetics; D011976:Receptors, Muscarinic / Q000235:genetics; D018041:Receptors, Neurokinin-2; D017981:Receptors, Neurotransmitter / Q000235:genetics*; D011985:Receptors, Serotonin / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013961:Thyroid Gland / Q000032:analysis; D014158:Transcription, Genetic
D011942:Receptors, Adrenergic, alpha; D011943:Receptors, Adrenergic, beta; D011976:Receptors, Muscarinic; D018041:Receptors, Neurokinin-2; D017981:Receptors, Neurotransmitter; D011985:Receptors, Serotonin; D004247:DNA; D004259:DNA-Directed DNA Polymerase; D019204:GTP-Binding Proteins