Membrane responses of B-16 melanoma cells to single exposure to ultraviolet light.
Electron spin resonance spectroscopy using the spin probe (5-, 12- and 16-deoxylstearic acid) was employed to analyze the changes in membrane fluidity in B-16 melanoma cells following UV-B exposure. The UV exposure resulted in the immediate accumulation of lipid peroxide, being accompanied by a change in membrane fluidity. The 12-DSA is the most sensitive to the changes in membrane organization caused by UV light. Na+,K+-ATPase activity was regulated by a change in membrane fluidity. Following UV exposure, the release of the prelabeled arachidonic acid from the cells was observed immediately. Ca2+-dependent calmodulin-dependent phospholipase A2-like activity was involved in the UV-stimulated arachidonic acid release from phospholipid.
Ogura
R
R
Department of Medical Biochemistry, Kurume University School of Medicine, Japan.
Sugiyama
M
M
Sakanashi
T
T
Suematsu
T
T
Hidaka
T
T
Morikawa
F
F
Kon
Y
Y
D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism / Q000528:radiation effects; D004578:Electron Spin Resonance Spectroscopy; D006801:Humans; D015227:Lipid Peroxidation / Q000528:radiation effects; D008544:Melanocytes / Q000378:metabolism / Q000528:radiation effects*; D008546:Melanoma, Experimental / Q000378:metabolism; D008560:Membrane Fluidity / Q000528:radiation effects*; D008563:Membrane Lipids / Q000528:radiation effects; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays
D001095:Arachidonic Acids; D008563:Membrane Lipids; D016718:Arachidonic Acid