title: Genetic basis of attenuation of the Sabin type 3 oral poliovirus vaccine.
abstract: The poliovirus type 3 Sabin oral poliovirus vaccine strain P3/Leon/12a1b differs in nucleotide sequence from its neurovirulent progenitor P3/Leon/37 by just 10 point mutations. The contribution of each mutation to the attenuation phenotype of the vaccine strain was determined by the construction of a series of recombinant viruses from infectious cDNA clones. The neurovirulence testing of recombinant viruses indicated that the attenuation phenotype is determined by just two point mutations: a C to U in the noncoding region at position 472 and a C to U at nucleotide 2034 which results in a serine-to-phenylalanine amino acid substitution in the structural protein VP3.
authors.affiliation: Department of Microbiology, University of Reading, United Kingdom.
authors.forename: G D
authors.initials: GD
authors.lastname: Westrop
authors.forename: K A
authors.initials: KA
authors.lastname: Wareham
authors.forename: D M
authors.initials: DM
authors.lastname: Evans
authors.forename: G
authors.initials: G
authors.lastname: Dunn
authors.forename: P D
authors.initials: PD
authors.lastname: Minor
authors.forename: D I
authors.initials: DI
authors.lastname: Magrath
authors.forename: F
authors.initials: F
authors.lastname: Taffs
authors.forename: S
authors.initials: S
authors.lastname: Marsden
authors.forename: M A
authors.initials: MA
authors.lastname: Skinner
authors.forename: G C
authors.initials: GC
authors.lastname: Schild
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001681:Biological Assay; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004252:DNA Mutational Analysis; D005814:Genes, Viral; D009420:Nervous System / Q000382:microbiology; D017955:Poliovirus / Q000235:genetics* / Q000472:pathogenicity; D011055:Poliovirus Vaccine, Oral / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D013329:Structure-Activity Relationship; D014613:Vaccines, Attenuated / Q000235:genetics*
chemical_list: D011055:Poliovirus Vaccine, Oral; D012367:RNA, Viral; D014613:Vaccines, Attenuated; D004247:DNA