UV irradiation inhibits initiation of DNA replication from oriC in Escherichia coli.
Irradiation of Escherichia coli with UV light causes a transient inhibition of DNA replication. This effect is generally thought to be accounted for by blockage of the elongation of DNA replication by UV-induced lesions in the DNA (a cis effect). However, by introducing an unirradiated E. coli origin (oriC)-dependent replicon into UV-irradiated cells, we have been able to show that the environment of a UV-irradiated cell inhibits initiation of replication from oriC on a dimer-free replicon. We therefore conclude that UV-irradiation of E. coli leads to a trans-acting inhibition of initiation of replication. The inhibition is transient and does not appear to be an SOS function.
Verma
M
M
Department of Biochemistry, University of Adelaide, S.A., Australia.
Moffat
K G
KG
Egan
J B
JB
D010582:Bacteriophage lambda / Q000235:genetics / Q000528:radiation effects; D004261:DNA Replication / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D004926:Escherichia coli / Q000235:genetics / Q000528:radiation effects*; D012093:Replicon / Q000528:radiation effects; D014466:Ultraviolet Rays; D014775:Virus Activation / Q000528:radiation effects; D014779:Virus Replication / Q000528:radiation effects