title: Reoviruses as probes of the gut mucosal T cell population.
abstract: Reovirus, serotype 1, causes a transient, asymptomatic infection of the murine intestine when given intraduodenally or orally. However, this infection markedly perturbs both B- and T- cell populations in Peyer's patches (PP) resulting in: 1) a rapid and persistent increase in specific precursors for cytotoxic T cells (pCTL) and a gradient of frequencies highest in PP and lowest in distal lymphoid tissue; 2) a similar increase in memory B cells committed to IgA; 3) the transient appearance of a subset of germinal center B cells identified by MAb, GC-T; 4) the appearance of pCTL among intraepithelial lymphocytes; and 5) the antigen non-specific alteration in Ig isotype potential of B cells previously primed and found in PP. The pCTL appearing upon acute gut mucosal infection with reovirus are Thyl+, Lyt2+, virus-specific, viral serotype non-specific, class I MHC haplotype restricted and occur within the subset of T cells which newly appears also identified by MAb GC-T. Infections of both neonatal and severe-combined immunodeficient mice indicate that the elements of the immune system may operate at many levels to resist, limit, contain, and resolve viral infection.
authors.affiliation: Department of Biology, University of Pennsylvania.
authors.forename: J J
authors.initials: JJ
authors.lastname: Cebra
authors.forename: J A
authors.initials: JA
authors.lastname: Cebra-Thomas
authors.forename: C F
authors.initials: CF
authors.lastname: Cuff
authors.forename: A
authors.initials: A
authors.lastname: George
authors.forename: S I
authors.initials: SI
authors.lastname: Kost
authors.forename: S D
authors.initials: SD
authors.lastname: London
authors.forename: D H
authors.initials: DH
authors.lastname: Rubin
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000145:classification / Q000276:immunology; D015415:Biomarkers; D004848:Epithelium / Q000276:immunology; D007410:Intestinal Diseases / Q000276:immunology* / Q000473:pathology; D007413:Intestinal Mucosa / Q000276:immunology / Q000473:pathology; D008221:Lymphoid Tissue / Q000276:immunology; D051379:Mice; D010581:Peyer's Patches / Q000276:immunology; D012088:Reoviridae Infections / Q000276:immunology* / Q000473:pathology; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
chemical_list: D015415:Biomarkers