Restriction endonuclease analysis of human cytomegalovirus isolated in immunocompromised patients.
Cytomegalovirus is frequently isolated from immunosuppressed patients or from patients with acquired immunodeficiency syndrome. Twelve patients were studied for several months. In 7 patients more than 1 Cytomegalovirus was isolated; in one of these patients three viruses were isolated, two of them show small differences in their migration pattern and the third seems to be a different strain after restriction analysis of CMV genome. This could reflect variation in the genome of the CMV virus as it has been already shown for viruses from other families in immunocompromised patients and shows the occurrence of multiple strains infection in a single patient.
Vega-Alvarez
M
M
Laboratoire de Virologie, Hôpital Trousseau, Paris, France.
Garbarg-Chenon
A
A
Goulin
J
J
Nicolas
J C
JC
Bricout
F
F
D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology*; D003587:Cytomegalovirus / Q000235:genetics* / Q000302:isolation & purification; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis*; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; D004591:Electrophoresis, Polyacrylamide Gel; D014644:Genetic Variation; D006801:Humans; D007108:Immune Tolerance; D016030:Kidney Transplantation
D004279:DNA, Viral; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII