Production of human warm-reacting red cell monoclonal autoantibodies by Epstein-Barr virus transformation.
Monoclonal antibody technology has been used in both murine and human systems to produce a variety of antibodies that react with the human red cell (RBC). RBC monoclonal autoantibodies have been obtained from animal models of autoimmune hemolytic anemia (AIHA), but to date no warm-reactive monoclonal autoantibodies have been generated from human B cells. Using the Epstein-Barr virus (EBV) transformation method, clones of RBC autoantibodies were generated from two patients with AIHA. These antibodies reacted preferentially at 37 degrees C, agglutinated or bound to a variety of different RBC phenotypes, and were IgM in nature. The serologic reactivity of one clone showed a relative specificity to e+ RBCs that was similar to that seen in the patient's serum. These results are the first to demonstrate that warm-reactive RBC autoantibodies can be obtained from patients with AIHA using the technique of EBV transformation, and they further substantiate the existence of warm-reactive IgM RBC autoantibodies in the spectrum of warm AIHA.
Andrzejewski
C
C
Department of Pathology and Laboratory Medicine, University of Pennsylvania, School of Medicine, Philadelphia.
Young
P J
PJ
Goldman
J
J
Spitalnik
S L
SL
Silberstein
L E
LE
D000328:Adult; D000744:Anemia, Hemolytic, Autoimmune / Q000097:blood; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D001323:Autoantibodies / Q000096:biosynthesis*; D015139:Blotting, Southern; D001831:Body Temperature; D002460:Cell Line; D002472:Cell Transformation, Viral; D002999:Clone Cells / Q000378:metabolism; D005260:Female; D004854:Herpesvirus 4, Human; D006801:Humans; D007525:Isoelectric Focusing; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D008875:Middle Aged
D000911:Antibodies, Monoclonal; D001323:Autoantibodies