The protein kinases tightly bound to DNA are present in normal tissues and in regenerating liver, but strongly decreased in hepatomas.
We have previously described in rat liver two protein kinases tightly bound to DNA, one is serine-specific, the other arginine-specific. In this work we show that both enzymes are present in various rat tissues and in liver from various species. Both kinase specific activities are strongly decreased in methyl-DBA-induced hepatomas and in HTC cells but not in regenerating liver after hepatectomy. This decrease is then not related to cell proliferation.
Levy-Favatier
F
F
Institut de Pathologie Moléculaire, Unité de Recherches Associée au CNRS URA 1147, Faculté de Médecine Cochin-Port-Royal, Paris, France.
Tichnonicky
L
L
Kruh
J
J
Delpech
M
M
D000818:Animals; D001120:Arginine / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D006498:Hepatectomy; D008114:Liver Neoplasms, Experimental / Q000201:enzymology* / Q000378:metabolism; D008115:Liver Regeneration; D008297:Male; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012694:Serine / Q000378:metabolism
D004268:DNA-Binding Proteins; D012694:Serine; D004247:DNA; D001120:Arginine; D011494:Protein Kinases