Phenotypic and functional alteration of CD4+ T cells after antigen stimulation. Resolution of two populations of memory T cells that both secrete interleukin 4.
Phenotypic and functional alteration of murine CD4+ T cells after antigenic stimulation was studied using two anti-T cell mAbs recently described that define four distinct T cell subsets. Activation of T cells resulted in the permanent loss of 3G11 expression. However, two phenotypically distinct memory T cell populations were established depending on the system used; whereas those for anti-KLH antibody response were enriched in the fraction expression 6C10 (Fr. III), memory T cells for the allogeneic MLR lacked such expression (Fr. IV). Furthermore, successive stimulation with antigen in vitro resulted in secretion of IL-4 without detectable IL-2. This alteration of phenotype and interleukin secretion was also demonstrable when starting with 3G11+6C10- cells (Fr. I), the fraction that secretes IL-2 exclusively upon activation.
Hayakawa
K
K
Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
Hardy
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D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology*; D000945:Antigens, Differentiation, T-Lymphocyte; D001402:B-Lymphocytes / Q000276:immunology; D006433:Hemocyanins / Q000276:immunology; D007117:Immunization, Secondary; D007156:Immunologic Memory; D007376:Interleukin-2 / Q000096:biosynthesis; D015847:Interleukin-4; D007378:Interleukins / Q000096:biosynthesis*; D007399:Interphase; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010641:Phenotype; D006377:T-Lymphocytes, Helper-Inducer / Q000145:classification* / Q000276:immunology / Q000502:physiology
D000941:Antigens; D000945:Antigens, Differentiation, T-Lymphocyte; D007376:Interleukin-2; D007378:Interleukins; D015847:Interleukin-4; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin