Inhibition of T-lymphocyte mitogenic responses and effects on cell functions by bovine herpesvirus 1.
The mitogenic response of bovine peripheral blood mononuclear cells stimulated by concanavalin A (ConA) was suppressed by infectious bovine herpesvirus 1 (BHV-1). Proliferation in response to interleukin-2 (IL-2) by IL-2-dependent lymphocyte cultures was also inhibited by BHV-1. Although inhibition of mitogenesis approached 100%, less than 1 cell in 1,000 was productively infected by BHV-1 in ConA-stimulated cultures. Neither conditioned medium from mitogen-stimulated peripheral blood mononuclear cell cultures nor human recombinant IL-2 reversed suppression by the virus. Infection by BHV-1 did not influence the expression of IL-2 or IL-2 receptor mRNA in ConA-stimulated cultures, nor did it affect the cytolytic capabilities of lymphocytes. The data suggest that the inhibition of T-lymphocyte proliferation is the result of a nonproductive BHV-1 infection.
Carter
J J
JJ
Program in Biochemistry and Biophysics, Washington State University, Pullman 99164-4340.
Weinberg
A D
AD
Pollard
A
A
Reeves
R
R
Magnuson
J A
JA
Magnuson
N S
NS
D000818:Animals; D002417:Cattle; D003602:Cytotoxicity, Immunologic; D004247:DNA / Q000096:biosynthesis; D004987:Ethers / Q000494:pharmacology; D007242:Herpesvirus 1, Bovine / Q000276:immunology*; D066298:In Vitro Techniques; D007241:Infectious Bovine Rhinotracheitis / Q000276:immunology; D007376:Interleukin-2 / Q000494:pharmacology / Q000502:physiology; D015759:Ionomycin; D008213:Lymphocyte Activation / Q000187:drug effects; D010703:Phorbol Esters / Q000494:pharmacology; D015375:Receptors, Interleukin-2 / Q000502:physiology; D013601:T-Lymphocytes / Q000276:immunology* / Q000382:microbiology
D004987:Ethers; D007376:Interleukin-2; D010703:Phorbol Esters; D015375:Receptors, Interleukin-2; D015759:Ionomycin; D004247:DNA