Nonimmunochemical quantitation of mammalian apolipoprotein A-I in whole serum or plasma by nonreducing gel electrophoresis.
A rapid and convenient method for the quantitation of mammalian apoA-I has been developed. The method involves nonreducing sodium dodecyl sulfate gel electrophoresis and Coomassie blue staining, and takes advantage of the relative abundance of apoA-I in whole serum or plasma. ApoA-I was sufficiently resolved to allow quantitation by laser densitometry or spectrophotometry. The assay was linear from 0.25 to 4.0 micrograms of apoA-I. Analytic recovery was 98%. Within-assay variability was 3.1% and between-assay variability was 7.5%. A high degree of positive correlative (r = 0.98) was observed with a human apoA-I radioimmunoassay. For several species investigated, the apoA-I values obtained correlated strongly and positively with high density lipoprotein cholesterol values. When applied to a study of nutritional perturbation in the Mongolian gerbil, the method detected sensible and significant changes in serum apoA-I that paralleled changes in HDL cholesterol.
France
D S
DS
Department of Lipid and Lipoprotein Metabolism, Sandoz Research Institute, East Hanover, NJ 07936.
Hughes
T E
TE
Miserendino
R
R
Spirito
J A
JA
Babiak
J
J
Eskesen
J B
JB
Tapparelli
C
C
Paterniti
J R
JR
D000818:Animals; D016632:Apolipoprotein A-I; D001054:Apolipoproteins A / Q000097:blood*; D015153:Blotting, Western; D002428:Cebus; D002791:Cholesterol, Dietary / Q000009:adverse effects; D008076:Cholesterol, HDL / Q000097:blood; D003720:Densitometry; D004041:Dietary Fats / Q000009:adverse effects; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D005849:Gerbillinae; D006041:Goats; D006801:Humans; D007834:Lasers; D008297:Male; D010949:Plasma / Q000032:analysis; D011817:Rabbits; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D015203:Reproducibility of Results; D012394:Rosaniline Dyes; D013045:Species Specificity
D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D002791:Cholesterol, Dietary; D008076:Cholesterol, HDL; D004041:Dietary Fats; D012394:Rosaniline Dyes; C048139:Coomassie blue