title: Two high-affinity interleukin 1 receptors represent separate gene products.
abstract: Interleukin 1 (IL-1) is a polypeptide hormone that mediates a broad range of biological activities and interacts with surface receptors on numerous cell types. Equilibrium binding studies have identified a class of IL-1 receptors on T cells, fibroblasts, and epithelial cells that have 2- to 5-fold higher affinity than the receptors on bone marrow cells, pre-B cells, and macrophage cell lines. Affinity cross-linking with human 125I-labeled IL-1 alpha (125I-IL-1 alpha) labels an approximately 100-kDa protein on T cells and fibroblasts and an approximately 80-kDa protein on pre-B cells and macrophage cell lines. Monoclonal and polyclonal antibodies specific for the IL-1 receptor on T cells and fibroblasts block human 125I-IL-1 alpha binding to T cells, fibroblasts, and epithelial cells but cannot block IL-1 binding to bone marrow cells, pre-B cells, and macrophages. These antibodies immunoprecipitate the IL-1 receptor-human 125I-IL-1 alpha complex from T cells and fibroblasts but not from pre-B cells and macrophage cell lines. An S1 nuclease protection assay demonstrated that T cells and fibroblasts contain identical IL-1 receptor mRNA but that pre-B cells and macrophages do not contain this receptor mRNA. Taken together, the data demonstrate that mouse T cells, fibroblasts, and epithelial cells express an identical IL-1 receptor, whereas the IL-1 receptor on pre-B cells, macrophages, and bone marrow cells represents a different gene product.
authors.affiliation: Department of Molecular Genetics, Roche Research Center, Hoffmann-La Roche Inc., Nutley, NJ 07110.
authors.forename: R
authors.initials: R
authors.lastname: Chizzonite
authors.forename: T
authors.initials: T
authors.lastname: Truitt
authors.forename: P L
authors.initials: PL
authors.lastname: Kilian
authors.forename: A S
authors.initials: AS
authors.lastname: Stern
authors.forename: P
authors.initials: P
authors.lastname: Nunes
authors.forename: K P
authors.initials: KP
authors.lastname: Parker
authors.forename: K L
authors.initials: KL
authors.lastname: Kaffka
authors.forename: A O
authors.initials: AO
authors.lastname: Chua
authors.forename: D K
authors.initials: DK
authors.lastname: Lugg
authors.forename: U
authors.initials: U
authors.lastname: Gubler
mesh_terms: D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D002478:Cells, Cultured; D005796:Genes; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D008970:Molecular Weight; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D011971:Receptors, Immunologic / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D017472:Receptors, Interleukin-1; D011994:Recombinant Proteins / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D007375:Interleukin-1; D012333:RNA, Messenger; D011948:Receptors, Antigen, T-Cell; D011971:Receptors, Immunologic; D017472:Receptors, Interleukin-1; D011994:Recombinant Proteins