Structure of the Drosophila DNA topoisomerase II gene. Nucleotide sequence and homology among topoisomerases II.
We have determined the nucleotide sequence of the Drosophila DNA topoisomerase II gene. Data from primer extension and S1 nuclease protection experiments were combined with comparisons of genomic and cDNA sequences to determine the structure of the mature messenger RNA. This message has a large open reading frame of 4341 nucleotides. The length of the predicted protein is 1447 amino acids with a molecular weight of 164,424. Topoisomerase II can be divided into three domains: (1) an N-terminal region with homology to the B (ATPase) subunit of the bacterial type II topoisomerase, DNA gyrase; (2) a central region with homology to the A (breaking and rejoining) subunit of DNA gyrase; (3) a C-terminal region characterized by alternating stretches of positively and negatively charged amino acids. DNA topoisomerase II from the fruit fly shares significant sequence homology with those from divergent sources, including bacteria, bacteriophage T4 and yeasts. The location and distribution of homologous stretches in these sequences are analyzed.
Wyckoff
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Department of Biochemistry, Duke University Medical Center, Durham, NC 27710.
Natalie
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D
Nolan
J M
JM
Lee
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M
Hsieh
T
T
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001412:Bacillus subtilis; D001483:Base Sequence; D004250:DNA Topoisomerases, Type II / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D004926:Escherichia coli; D005796:Genes; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D012440:Saccharomyces; D012689:Sequence Homology, Nucleic Acid
D000596:Amino Acids; D012333:RNA, Messenger; D004250:DNA Topoisomerases, Type II