Membrane proteins in senescent erythrocytes.
The examination of erythrocyte senescence has been facilitated by recent advances in techniques for the isolation of aged red cells. One of these methods, which uses biotinylated rabbit erythrocytes, has been used to examine the state of membrane proteins in effete cells. These aged red cells were found to have normal ratios of alpha-spectrin and beta-spectrin as well as normal levels of ankyrin. The observation concerning ankyrin is particularly important due to the sensitivity of this protein to proteolysis and the postulated action of proteinases in the aging process. The senescent erythrocytes were also found to have an altered ratio of bands 4.1a and 4.1b without any apparent change in the total level of 4.1. In addition, the analysis of the aged cell membranes did not show any large-molecular-mass aggregated protein at the origin of the SDS/polyacrylamide gels, indicating a lack of transglutaminase activity in the senescence process for rabbit erythrocytes. These results indicate that aging of the rabbit erythrocyte is not accompanied by gross proteolytic degradation or transglutaminase-catalysed cross-linking of membrane components.
Suzuki
T
T
Department of Basic and Clinical Research, Research Institute of Scripps Clinic, La Jolla, CA 92037.
Dale
G L
GL
D000818:Animals; D017487:Ankyrins; D001798:Blood Proteins / Q000032:analysis; D003598:Cytoskeletal Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D004905:Erythrocyte Aging; D004910:Erythrocyte Membrane / Q000032:analysis*; D008565:Membrane Proteins / Q000032:analysis / Q000097:blood*; D009479:Neuropeptides; D011817:Rabbits; D013049:Spectrin / Q000032:analysis
D017487:Ankyrins; D001798:Blood Proteins; D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; D009479:Neuropeptides; C028841:erythrocyte membrane band 4.1 protein; C044791:erythrocyte membrane band 4.2 protein; C120557:erythrocyte membrane protein band 4.1-like 1; D013049:Spectrin