title: Phosphorylation of bovine hormone-sensitive lipase by the AMP-activated protein kinase. A possible antilipolytic mechanism.
abstract: Hormone-sensitive lipase is phosphorylated at a single site (site 2) in vitro by the AMP-activated protein kinase, without any direct effect on the activity of the enzyme. The amino acid sequence around this site has been determined. Ca2+/calmodulin-dependent protein kinase II also phosphorylates hormone-sensitive lipase predominantly at this site, whilst cyclic-GMP-dependent protein kinase phosphorylates exclusively the regulatory site (site 1) which is also phosphorylated by cyclic-AMP-dependent protein kinase. Phosphorylation of site 2 has been found to inhibit subsequent phosphorylation and activation of hormone-sensitive lipase by the cyclic-AMP-dependent and cyclic-GMP-dependent protein kinases, indicating that site-2 phosphorylation may have an antilipolytic role in vivo.
authors.affiliation: Department of Biochemistry and Genetics, Medical School, University of Newcastle Upon Tyne, England.
authors.forename: A J
authors.initials: AJ
authors.lastname: Garton
authors.forename: D G
authors.initials: DG
authors.lastname: Campbell
authors.forename: D
authors.initials: D
authors.lastname: Carling
authors.forename: D G
authors.initials: DG
authors.lastname: Hardie
authors.forename: R J
authors.initials: RJ
authors.lastname: Colbran
authors.forename: S J
authors.initials: SJ
authors.lastname: Yeaman
mesh_terms: D000818:Animals; D001665:Binding Sites; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D008066:Lipolysis; D010748:Phosphopeptides / Q000302:isolation & purification; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism* / Q000494:pharmacology; D002787:Sterol Esterase / Q000378:metabolism*
chemical_list: D010748:Phosphopeptides; D011494:Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D002265:Carboxylic Ester Hydrolases; D002787:Sterol Esterase