title: The separate roles of PQQ and apo-enzyme syntheses in the regulation of glucose dehydrogenase activity in Klebsiella pneumoniae NCTC 418.
abstract: No holoenzyme pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase and only very low apoenzyme levels could be detected in cells of Klebsiella pneumoniae, growing anaerobically, or carrying out a fumarate or nitrate respiration. Low glucose dehydrogenase activity in some aerobic glucose-excess cultures of K. pneumoniae (ammonia or sulphate limitation) was increased significantly by addition of PQQ, whereas in cells already possessing a high glucose dehydrogenase activity (phosphate or potassium limitation) extra PQQ had almost no effect. These observations indicate that the glucose dehydrogenase activity in K. pneumoniae is modulated by both PQQ synthesis and synthesis of the glucose dehydrogenase apo-enzyme.
authors.affiliation: Department of Microbiology, University of Amsterdam, The Netherlands.
authors.forename: R W
authors.initials: RW
authors.lastname: Hommes
authors.forename: P T
authors.initials: PT
authors.lastname: Herman
authors.forename: P W
authors.initials: PW
authors.lastname: Postma
authors.forename: D W
authors.initials: DW
authors.lastname: Tempest
authors.forename: O M
authors.initials: OM
authors.lastname: Neijssel
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D001051:Apoenzymes / Q000096:biosynthesis*; D001059:Apoproteins / Q000096:biosynthesis*; D002237:Carbohydrate Dehydrogenases / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D003067:Coenzymes / Q000096:biosynthesis*; D005942:Gluconates / Q000096:biosynthesis; D005947:Glucose / Q000378:metabolism; D005948:Glucose Dehydrogenases / Q000096:biosynthesis / Q000378:metabolism*; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000254:growth & development; D010101:Oxygen Consumption; D045542:PQQ Cofactor; D015363:Quinolones / Q000096:biosynthesis*
chemical_list: D001051:Apoenzymes; D001059:Apoproteins; D003067:Coenzymes; D005942:Gluconates; D015363:Quinolones; D002245:Carbon Dioxide; C008229:2-ketogluconate; D045542:PQQ Cofactor; D002237:Carbohydrate Dehydrogenases; D005948:Glucose Dehydrogenases; D005947:Glucose