Vanadate inhibition of mitochondrial respiration and H+ ATPase activity in Saccharomyces cerevisiae.
The effects of vanadate on mitochondrial respiration and H+ ATPase activity in Saccharomyces cerevisiae were studied. A 50% inhibition of oxygen uptake in isolated mitochondria was produced by 4.4 mM-V2O5. Activity of H+ ATPase in whole mitochondria was inhibited by 50% by 5.5 microM-V2O5, in submitochondrial particles by 55 microM-V2O5; and in the chloroform-released H+ ATPase by 0.5 mM-V2O5. Vanadate was also found to relieve growth inhibition caused by the mitochondrial H+ ATPase inhibitors NN'-decyclohexylcarbodiimide and oligomycin. These results imply that vanadate could affect mitochondrial respiration by interacting with the H+ ATPase in S. cerevisiae.
Henderson
G E
GE
School of Biological Sciences and Environmental Health, Thames Polytechnic, London, U.K.
Evans
I H
IH
Bruce
I J
IJ
D000251:Adenosine Triphosphatases / Q000032:analysis; D008928:Mitochondria / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D010101:Oxygen Consumption / Q000187:drug effects; D006180:Proton-Translocating ATPases / Q000032:analysis / Q000037:antagonists & inhibitors*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D014638:Vanadates / Q000494:pharmacology*
D014638:Vanadates; D000251:Adenosine Triphosphatases; D006180:Proton-Translocating ATPases