Interferon and (2'-5')oligoadenylate enhance the expression of low affinity receptors for IgE (Fc epsilon R2/CD23) on the human monoblast cell line U937.
The regulation of low affinity IgE receptor (Fc epsilon R2/CD23) expression on the human monoblast cell line U937 was examined by an anti-Fc epsilon R2/CD23 monoclonal antibody (H107) and the cDNA probe for Fc epsilon R2/CD23. Alpha interferon (IFN-alpha) and its intracellular mediator, (2'-5')oligoadenylate (2, 5-A), induced Fc epsilon R2/CD23 expression on U937 with no significant increase of the Fc epsilon R2/CD23 mRNA. PMA and IFN-gamma increased both surface Fc epsilon R2/CD23 expression and the Fc epsilon R2/CD23 mRNA levels. IFN-alpha effectively induced 2, 5-A synthetase activity in U937 cells, whereas IFN-gamma induced little. The results suggest that the mechanisms of enhancement of Fc epsilon R2/CD23 expression on U937 cells by IFN-alpha and IFN-gamma are different and that 2, 5-A may play an important role in the Fc epsilon R2/CD23 expression on U937 cells induced by IFN-alpha.
Mayumi
M
M
Department of Pediatrics, Faculty of Medicine, Kyoto University, Japan.
Kawabe
T
T
Nishioka
H
H
Tanaka
M
M
Kim
K M
KM
Heike
T
T
Yodoi
J
J
Mikawa
H
H
D015088:2',5'-Oligoadenylate Synthetase / Q000378:metabolism; D000227:Adenine Nucleotides / Q000494:pharmacology*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis*; D015152:Blotting, Northern; D002460:Cell Line; D003907:Dexamethasone / Q000494:pharmacology; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism*; D007370:Interferon Type I / Q000494:pharmacology*; D009843:Oligoribonucleotides / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D011961:Receptors, Fc / Q000032:analysis*; D017455:Receptors, IgE
D000227:Adenine Nucleotides; D000944:Antigens, Differentiation, B-Lymphocyte; D007370:Interferon Type I; D009843:Oligoribonucleotides; D012333:RNA, Messenger; D011961:Receptors, Fc; D017455:Receptors, IgE; D007073:Immunoglobulin E; C023505:2',5'-oligoadenylate; D003907:Dexamethasone; D015088:2',5'-Oligoadenylate Synthetase