A rapid microneutralization assay for the measurement of neutralizing antibody reactive with human cytomegalovirus.
We have developed a murine monoclonal antibody reactive with a major immediate early 72,000 dalton protein of human cytomegalovirus and utilized this reagent in a rapid virus titration and microneutralization assay. Because of the early expression of this virus encoded protein, both assays could be accomplished within 16 h following virus inoculation. In addition, both assays resulted in considerable savings of reagents because the assays were carried out in 96-well microtiter plates. These assays should prove useful in the preparation and study of neutralizing antibodies directed against human cytomegalovirus.
Andreoni
M
M
Department of Infectious Diseases, 2nd University of Rome, Italy.
Faircloth
M
M
Vugler
L
L
Britt
W J
WJ
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000276:immunology; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000276:immunology*; D005347:Fibroblasts; D006801:Humans; D017874:Immediate-Early Proteins; D015151:Immunoblotting; D009500:Neutralization Tests; D009687:Nuclear Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D017874:Immediate-Early Proteins; D009687:Nuclear Proteins; C044582:immediate-early proteins, cytomegalovirus