Cloning and expression of a novel rat GABAA receptor.
Two full-length cDNA clones encoding alpha- and beta-subunits of a GABAA receptor have been isolated from a rat cerebral cortex cDNA library. The mature alpha-subunit protein consists of 428 amino acids with a calculated Mr of 48,680. This protein is highly homologous (approximately 99% amino acid identity) with the bovine brain alpha 1-subunit receptor [(1988) Nature 335, 76-79]. The mature rat beta-subunit receptor is a 448 amino acid polypeptide and shares approximately 80% amino acid identity with the previously characterized bovine GABAA receptor beta-subunit [(1987) Nature 328, 221-227]. Co-expression of the cloned DNA in Xenopus oocytes produces a functional receptor and ion channel with pharmacological characteristics of a GABAA receptor. GABAA alpha- and beta-subunit mRNA is detectable in the cortex, cerebellum and hippocampus.
Lolait
S J
SJ
Laboratory of Cell Biology, NIMH 20892.
O'Carroll
A M
AM
Kusano
K
K
Muller
J M
JM
Brownstein
M J
MJ
Mahan
L C
LC
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002531:Cerebellum / Q000032:analysis; D002540:Cerebral Cortex / Q000032:analysis; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005786:Gene Expression Regulation; D006031:Glycosylation; D006624:Hippocampus / Q000032:analysis; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D009865:Oocytes / Q000378:metabolism; D021382:Protein Sorting Signals; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D011963:Receptors, GABA-A / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection; D014981:Xenopus; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D021382:Protein Sorting Signals; D012333:RNA, Messenger; D011963:Receptors, GABA-A; D005680:gamma-Aminobutyric Acid; D004247:DNA