Regulation of the S100 protein and GFAP genes is mediated by two common mechanisms in RT4 neuro-glial cell lines.
RT4-AC cells express both neuronal and glial properties and undergo cell-type conversion in culture to three distinct derivatives, described as either neuronal-like or glial-like. A coordinate induction of glial fibrillary acidic protein (GFAP) and S100 protein and GFAP gene expression is coordinately induced by cAMP. In addition, for the first time we provide direct evidence that the ability to express both the S100 and GFAP genes is conserved with cell-type conversion to the glial derivative cell types, but is coordinately lost with conversion to the neuronal derivative cell types. These results make it highly likely that the GFAP and S100 genes are regulated by two common mechanisms in RT4-AC cells: (1) cAMP-mediated control of gene expression; and (2) a mechanism that allows these two genes to be coordinately expressed or not expressed as a consequence of cell-type conversion.
Freeman
M R
MR
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309.
Beckmann
S L
SL
Sueoka
N
N
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000502:physiology; D004273:DNA, Neoplasm / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D005796:Genes; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics*; D015151:Immunoblotting; D009457:Neuroglia; D009474:Neurons; D010524:Peripheral Nervous System Neoplasms; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D051381:Rats; D009418:S100 Proteins / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects
D004273:DNA, Neoplasm; D005904:Glial Fibrillary Acidic Protein; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D009418:S100 Proteins; D005576:Colforsin; D003994:Bucladesine; D000242:Cyclic AMP