Dual rearrangement of immunoglobulin and T-cell receptor genes in blast crisis of CML.
Dual rearrangement of immunoglobulin and T-cell antigen receptor (beta, delta) genes was demonstrated in a case of Philadelphia chromosome-positive chronic myeloid leukemia (CML) in blast crisis. The blast cells, showing L2 morphology and high activity of TdT, expressed pre-B cell (CD19+, Ia+) and myeloid (CD13+, CD34+) surface antigens but lacket T-cell antigens (CD2-, CD7-). Cytogenetic studies on bone marrow and peripheral blood revealed the Phl chromosome in all metaphases analyzed, majority of which also had the additional chromosome changes, +8, +10, +21. Furthermore, molecular analysis of the breakpoint cluster region (bcr) on chromosome 22 showed a rearrangement, confirming the CML origin of the blast cells.
Uike
N
N
Department of Hematology, National Kyushu Cancer Center Hospital, Japan.
Takeichi
N
N
Kimura
N
N
Takahira
H
H
Kozuru
M
M
D001752:Blast Crisis / Q000201:enzymology / Q000235:genetics*; D002879:Chromosomes, Human, Pair 10; D002891:Chromosomes, Human, Pair 21; D002898:Chromosomes, Human, Pair 8; D004253:DNA Nucleotidylexotransferase / Q000097:blood; D015329:Gene Rearrangement, T-Lymphocyte; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D005803:Genes, Immunoglobulin; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007138:Immunoglobulin delta-Chains / Q000235:genetics; D007621:Karyotyping; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged
D007143:Immunoglobulin Heavy Chains; D007138:Immunoglobulin delta-Chains; D004253:DNA Nucleotidylexotransferase