title: [Evaluation of a biotinylated probe for identification of Neisseria gonorrhoeae].
abstract: We evaluated a DNA biotinylated probe for the rapid identification of Neisseria gonorrhoeae in culture (Ortho Diagnostics System, Raritan, NJ). Twenty-one strains of N. gonorrhoeae, including type strain, 57 strains of other Neisseria species, and 104 strains of other genera were studied. The probe was highly sensitive (100%) and specific (96%). All N. gonorrhoeae strains gave strong signals, and only two cross-reaction were observed with N. lactamica, which has a close genetic relationship to N. gonorrhoeae. Our results indicate that specific recombinant DNA probe should offer a reliable and rapid method for routine diagnosis of Neisseria gonorrhoeae.
authors.affiliation: Laboratoire de Microbiologie et d'Hygiène Hospitalière, Hpital Salvator, Sainte-Marguerite, MARSEILLE, France.
authors.forename: C
authors.initials: C
authors.lastname: Bollet
authors.forename: M
authors.initials: M
authors.lastname: Grandguillot
authors.forename: H
authors.initials: H
authors.lastname: Bouchemal
authors.forename: N
authors.initials: N
authors.lastname: Boutefnouchet
authors.forename: P
authors.initials: P
authors.lastname: de Micco
mesh_terms: D001710:Biotin; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D005189:False Positive Reactions; D009344:Neisseria gonorrhoeae / Q000235:genetics / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial; D001710:Biotin
vernacular_title: Evaluation d'une sonde biotinylée pour l'identification de Neisseria gonorrhoeae.