Immunoelectron microscopy of Bacillus subtilis cells secreting human interferon alpha 1 or staphylokinase.
Post-embedding labelling techniques with colloidal gold-IgG or -protein A complexes were used to determine the subcellular location of IFN alpha 1 and staphylokinase secreted from Bacillus subtilis GB500 cells. Both proteins were present in the cytoplasma and the cell envelope pointing to a posttranslational mode of translocation across the cytoplasmic membrane. 5- to 10-fold higher concentrations of gold particles per 0.1 micron 2 were found on the cell envelope and clustering was observed suggesting preferential regions for secretion sites. Several control experiments ensured the specificity of the labelling data.
Wagner
B
B
Central Institute of Microbiology and Experimental Therapy, Academy of Sciences of G.D.R., Jena, D.D.R.
Wagner
M
M
Wollweber
L
L
Behnke
D
D
D001412:Bacillus subtilis / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D001692:Biological Transport; D006801:Humans; D007150:Immunohistochemistry; D007370:Interferon Type I / Q000378:metabolism*; D008666:Metalloendopeptidases / Q000378:metabolism*; D008854:Microscopy, Electron
D007370:Interferon Type I; D008666:Metalloendopeptidases; C022653:auR protein, Staphylococcus aureus