title: Cloning the interleukin 1 receptor from human T cells.
abstract: cDNA clones of the interleukin 1 (IL-1) receptor expressed in a human T-cell clone have been isolated by using a murine IL-1 receptor cDNA as a probe. The human and mouse receptors show a high degree of sequence conservation. Both are integral membrane proteins possessing a single membrane-spanning segment. Similar to the mouse receptor, the human IL-1 receptor contains a large cytoplasmic region and an extracellular, IL-1 binding portion composed of three immunoglobulin-like domains. When transfected into COS cells, the human IL-1 receptor cDNA clone leads to expression of two different affinity classes of receptors, with Ka values indistinguishable from those determined for IL-1 receptors in the original T-cell clone. An IL-1 receptor expressed in human dermal fibroblasts has also been cloned and sequenced and found to be identical to the IL-1 receptor expressed in T cells.
authors.affiliation: Immunex Corp., Seattle, WA 98101.
authors.forename: J E
authors.initials: JE
authors.lastname: Sims
authors.forename: R B
authors.initials: RB
authors.lastname: Acres
authors.forename: C E
authors.initials: CE
authors.lastname: Grubin
authors.forename: C J
authors.initials: CJ
authors.lastname: McMahan
authors.forename: J M
authors.initials: JM
authors.lastname: Wignall
authors.forename: C J
authors.initials: CJ
authors.lastname: March
authors.forename: S K
authors.initials: SK
authors.lastname: Dower
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005347:Fibroblasts / Q000276:immunology; D015870:Gene Expression; D005796:Genes; D006801:Humans; D007375:Interleukin-1 / Q000276:immunology* / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D008969:Molecular Sequence Data; D011948:Receptors, Antigen, T-Cell / Q000235:genetics; D011971:Receptors, Immunologic / Q000235:genetics* / Q000378:metabolism; D017472:Receptors, Interleukin-1; D012689:Sequence Homology, Nucleic Acid; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D007375:Interleukin-1; D011948:Receptors, Antigen, T-Cell; D011971:Receptors, Immunologic; D017472:Receptors, Interleukin-1; D004247:DNA