title: The Escherichia coli enterobactin biosynthesis gene, entD: nucleotide sequence and membrane localization of its protein product.
abstract: The nucleotide sequence of the Escherichia coli enterobactin biosynthesis gene entD has been determined. entD specifies a predicted 23579 Dalton protein containing several helical regions, a transmembrane segment and one positively charged domain. The EntD polypeptide was overexpressed and identified in electrophoretic gels as a membrane protein. Although results of conventional membrane fractionation techniques were inconclusive, protease accessibility studies provided evidence that EntD domains are exposed on the inner leaflet of the cytoplasmic membrane. The presence of repetitive extragenic palindromic (REP) sequences within the fepA-entD intercistronic region was confirmed. Lack of a canonical promoter and an iron control region 5' to entD, along with RNA hybridization data, suggest that an iron-regulated transcript contains both fepA and entD.
authors.affiliation: Department of Microbiology, School of Medicine, University of Missouri-Columbia 65212.
authors.forename: S K
authors.initials: SK
authors.lastname: Armstrong
authors.forename: G S
authors.initials: GS
authors.lastname: Pettis
authors.forename: L J
authors.initials: LJ
authors.lastname: Forrester
authors.forename: M A
authors.initials: MA
authors.lastname: McIntosh
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D004591:Electrophoresis, Polyacrylamide Gel; D019286:Endopeptidase K; D004758:Enterobactin / Q000032:analysis / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D015151:Immunoblotting; D008565:Membrane Proteins / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012694:Serine / Q000031:analogs & derivatives*; D012697:Serine Endopeptidases / Q000378:metabolism; D013104:Spheroplasts / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D008565:Membrane Proteins; D004758:Enterobactin; D012694:Serine; D012697:Serine Endopeptidases; D019286:Endopeptidase K