Porcine brain natriuretic peptide receptor in bovine adrenal cortex.
The action of porcine brain natriuretic peptide (pBNP) on the steroidogenesis was investigated in cultured bovine adrenocortical cells. Porcine BNP induced a significant dose-dependent inhibition of both ACTH- and A II-stimulated aldosterone secretion. 10(-8) M and 10(-7) M pBNP also significantly inhibited ACTH-stimulated cortisol and dehydroepiandrosterone (DHEA) secretions. Binding studies of [125I]-pBNP to bovine adrenocortical membrane fractions showed that adrenal cortex had high-affinity and low-capacity pBNP binding sites, with a dissociation constant (Kd) of 1.70 x 10(-10) M and a maximal binding capacity (Bmax) of 19.9 fmol/mg protein. Finally, the 135 Kd radioactive band was specially visualized in the affinity labeling of bovine adrenal cortex with disuccinimidyl suberate (DSS). These results suggest that pBNP may have receptor-mediated suppressive actions on bovine adrenal steroidogenesis, similar to that in atrial natriuretic peptide (ANP).
Higuchi
K
K
Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Hashiguchi
T
T
Ohashi
M
M
Takayanagi
R
R
Haji
M
M
Matsuo
H
H
Nawata
H
H
D000302:Adrenal Cortex / Q000187:drug effects / Q000378:metabolism*; D000345:Affinity Labels; D000450:Aldosterone / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003687:Dehydroepiandrosterone / Q000378:metabolism; D006854:Hydrocortisone / Q000378:metabolism; D008970:Molecular Weight; D020097:Natriuretic Peptide, Brain; D009419:Nerve Tissue Proteins / Q000378:metabolism* / Q000494:pharmacology; D011869:Radioligand Assay; D017461:Receptors, Atrial Natriuretic Factor; D011956:Receptors, Cell Surface / Q000378:metabolism*
D000345:Affinity Labels; D009419:Nerve Tissue Proteins; D011956:Receptors, Cell Surface; C058930:brain natriuretic peptide receptor; D020097:Natriuretic Peptide, Brain; D003687:Dehydroepiandrosterone; D000450:Aldosterone; D017461:Receptors, Atrial Natriuretic Factor; D006854:Hydrocortisone