Synergistic inhibition of oxidation in dispersed phosphatidylcholine liposomes by a combination of vitamin E and cysteine.
Oxidations of soybean phosphatidylcholine liposomes in an aqueous dispersion initiated by free radicals generated initially either in the aqueous phase or in the lipid phase were efficiently suppressed by vitamin E in the membranes. Vitamin E was consumed linearly with time and, when the inhibition period was over the oxidation proceeded rapidly at a rate similar to that in the absence of vitamin E. L-Cysteine was also effective by itself in scavenging radicals in the aqueous region, but it was consumed more rapidly than vitamin E. On the other hand, cysteine could not scavenge the radicals efficiently in a lipid region. Nevertheless, when vitamin E was incorporated into liposomes, the addition of cysteine in the aqueous phase prolonged the inhibition period and it reduced the rate of decay of vitamin E markedly even when the radicals were generated initially in the lipid bilayer. Furthermore, it was found by an electron spin resonance study that chromanoxyl radical disappeared quite rapidly when it was mixed with cysteine and that the spin adduct of cysteine radical was observed in the presence of alpha-(4-pyridyl-N-oxide)-N-tert-butyl nitrone. It was concluded that L-cysteine located in an aqueous region could regenerate vitamin E by reacting with vitamin E radical formed in a lipid region and show a synergistic antioxidant effect, although its efficiency of vitamin E regeneration was lower than that by vitamin C.
Motoyama
T
T
Department of Pediatrics, Osaka Medical College, Japan.
Miki
M
M
Mino
M
M
Takahashi
M
M
Niki
E
E
D000975:Antioxidants / Q000494:pharmacology; D003545:Cysteine / Q000378:metabolism / Q000494:pharmacology*; D004357:Drug Synergism; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D007700:Kinetics; D008081:Liposomes / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D010101:Oxygen Consumption; D010713:Phosphatidylcholines / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000494:pharmacology; D014810:Vitamin E / Q000378:metabolism / Q000494:pharmacology*
D000975:Antioxidants; D005609:Free Radicals; D008081:Liposomes; D010713:Phosphatidylcholines; D013438:Sulfhydryl Compounds; D014810:Vitamin E; D003545:Cysteine