title: Vanadate counteracts glucagon effects in isolated rat hepatocytes.
abstract: The incubation of isolated rat hepatocytes with vanadate increased the concentration of fructose 2,6-bisphosphate without modifying 6-phosphofructo-2-kinase activity. Vanadate also reverted and prevented the decrease of fructose 2,6-bisphosphate levels, of the "active" form of the 6-phosphofructo 2-kinase and of the pyruvate kinase activity ratio produced by glucagon, by probably counteracting the increase in cyclic AMP concentration.
authors.affiliation: Departament de Ciències Fisiològiques Humanes i de la Nutrició, Universitat de Barcelona, Zona Universitària, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Miralpeix
authors.forename: J
authors.initials: J
authors.lastname: Gil
authors.forename: J L
authors.initials: JL
authors.lastname: Rosa
authors.forename: J
authors.initials: J
authors.lastname: Carreras
authors.forename: R
authors.initials: R
authors.lastname: Bartrons
mesh_terms: D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D005215:Fasting; D005502:Food; D005635:Fructosediphosphates / Q000378:metabolism; D005934:Glucagon / Q000494:pharmacology*; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010732:Phosphofructokinase-1 / Q000378:metabolism; D010766:Phosphorylation; D011770:Pyruvate Kinase / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014638:Vanadates / Q000494:pharmacology*
chemical_list: D005635:Fructosediphosphates; D014638:Vanadates; C027652:fructose 2,6-diphosphate; D005934:Glucagon; D000242:Cyclic AMP; D010732:Phosphofructokinase-1; D011770:Pyruvate Kinase