The functional catalytic unit involved in proton pumping by rat liver cytochrome-c reductase and by cytochrome-c oxidase.
The effect of partial inhibition on the protonmotive stoichiometry of cytochrome-c reductase and cytochrome-c oxidase in intact rat liver mitochondria was examined using myxothiazol and cyanide as inhibitors, respectively. No decrease in the stoichiometry of either enzyme was found. It is shown that this result is consistent with the individual electron transfer units in each case being fully coupled to proton translocation but not with pairs of electron transfer units working in concert in dimers.
Moody
A J
AJ
Glynn Research Institute, Bodmin, Cornwall, U.K.
Rich
P R
PR
D000818:Animals; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology; D003579:Cytochrome Reductases / Q000378:metabolism*; D004579:Electron Transport; D003576:Electron Transport Complex IV / Q000037:antagonists & inhibitors / Q000378:metabolism*; D005033:Ethylmaleimide / Q000494:pharmacology; D005292:Ferricyanides / Q000378:metabolism / Q000494:pharmacology; D007700:Kinetics; D008689:Methacrylates; D008930:Mitochondria, Liver / Q000201:enzymology*; D009245:NADH Dehydrogenase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010101:Oxygen Consumption; D011522:Protons; D051381:Rats; D013844:Thiazoles / Q000494:pharmacology
D005292:Ferricyanides; D008689:Methacrylates; D011522:Protons; D013844:Thiazoles; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; C030517:myxothiazol; D003579:Cytochrome Reductases; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV; D005033:Ethylmaleimide; C028033:potassium ferricyanide