title: Suppression of autologous mixed leukocyte reaction in type 1 diabetes mellitus by in vivo-activated T lymphocytes.
abstract: In vivo-activated interleukin 2 receptor-positive T lymphocytes (Tac cells) are demonstrable and the autologous mixed leukocyte reaction (AMLR) is impaired in several autoimmune diseases, including type 1 insulin-dependent diabetes mellitus (IDDM). We investigated AMLR in greater detail, together with possible relationships between AMLR and Tac lymphocytes in IDDM. Coculture experiments with HLA identical patient-healthy sibling pairs revealed that both responder and stimulator cells of diabetic patients function abnormally in AMLR. Suppressive Tac lymphocytes among responder T cells seemed to impair their proliferation. The removal of Tac cells by immunomagnetic beads led to a striking enhancement of proliferation, while the restoration of AMLR cultures with enriched Tac cells was accompanied by a diminished response. The reasons for the poor stimulatory capacity of patient cells are at present unknown but may be due to altered function and/or structure of HLA-DR molecules.
authors.affiliation: Department of Biomedical Sciences, University of Tampere, Finland.
authors.forename: L
authors.initials: L
authors.lastname: Räsänen
authors.forename: H
authors.initials: H
authors.lastname: Hyöty
authors.forename: M
authors.initials: M
authors.lastname: Lehto
authors.forename: O P
authors.initials: OP
authors.lastname: Kallioniemi
authors.forename: T
authors.initials: T
authors.lastname: Huupponen
authors.forename: E
authors.initials: E
authors.lastname: Tuomilehto-Wolf
authors.forename: E
authors.initials: E
authors.lastname: Kaprio
authors.forename: P
authors.initials: P
authors.lastname: Leinikki
mesh_terms: D002478:Cells, Cultured; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics / Q000276:immunology*; D005190:Family; D006680:HLA Antigens / Q000032:analysis / Q000235:genetics; D006239:Haplotypes; D006801:Humans; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D015375:Receptors, Interleukin-2 / Q000032:analysis; D012016:Reference Values; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*
chemical_list: D006680:HLA Antigens; D015375:Receptors, Interleukin-2