[Demonstration of pyrimidine nucleoside phosphorylases in breast cancer].
Two pyrimidine phosphorylase activities have been isolated from the cytosol of cultivated MCF-7 cells of a human breast cancer, by ion exchange chromatography. Both enzymes are responsible for the cleavage of thymidine into thymine and deoxyribose-1-phosphate, for the synthesis of thymidine and for the transfer of deoxyribose from d-uridine to thymine. These activities are likely to participate in the regulation of the pool of pyrimidine nucleosides required for DNA synthesis.
Malette
P
P
Centre Régional de Recherche en Endocrinologie, Faculté de Médecine et Pharmacie, Rennes.
Sampérez
S
S
Jouan
P
P
D001943:Breast Neoplasms / Q000201:enzymology*; D002852:Chromatography, Ion Exchange; D003600:Cytosol / Q000201:enzymology; D006801:Humans; D010430:Pentosyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D057845:Pyrimidine Phosphorylases; D012267:Ribosemonophosphates / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D013941:Thymine / Q000378:metabolism; D014407:Tumor Cells, Cultured
D012267:Ribosemonophosphates; D010430:Pentosyltransferases; D057845:Pyrimidine Phosphorylases; D013941:Thymine; D013936:Thymidine
Mise en évidence de pyrimidine nucléoside phosphorylases dans les cancers du sein.