A study of shaggy reveals spatial domains of expression of achaete-scute alleles on the thorax of Drosophila.
A study of shaggy mutant clones on the notum reveals that a greater number of cells are diverted into the bristle pathway of differentiation and fewer cells remain to produce the epidermis, shaggy clones differentiate supernumerary microchaetae and macrochaetae but these are found in the correct spatial locations, e.g. clusters of macrochaetae are formed round the position of the extant macrochaetae. The shaggy mutant phenotype requires the functioning of the genes of the achaete-scute (AS-C) complex but a dosage study shows that it is unlikely that the AS-C is overexpressed in shaggy cells. Data are presented that argue, also, for a correct spatial expression of the AS-C in shaggy mutants. A study of clones doubly mutant for shaggy and different achaete and scute alleles is consistent with the hypothesis that the clusters of macrochaetae formed by shaggy represent the restricted spatial domains of expression of the AS-C. The results can be reconciled with the known role for the AS-C, in determining which bristle types differentiate where, and a role for shaggy in the cell interactions, within domains of the AS-C expression, leading to the definition of only one bristle mother cell.
Simpson
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Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Faculté de Médecine, Strasbourg, France.
Carteret
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C
D000483:Alleles; D000818:Animals; D002454:Cell Differentiation; D004303:Dosage Compensation, Genetic; D004331:Drosophila melanogaster / Q000033:anatomy & histology / Q000235:genetics*; D000078404:Epidermal Cells; D015870:Gene Expression; D005838:Genotype; D009154:Mutation; D010641:Phenotype; D011995:Recombination, Genetic / Q000528:radiation effects; D013909:Thorax / Q000033:anatomy & histology