title: Nodule-specific kinases phosphorylating nuclear factors in isolated nuclei.
abstract: In vitro phosphorylation of total nuclear proteins from soybean (Glycine max L) nodules formed by Bradyrhizobium japonicum 61A76 showed several differences in comparison with those from uninfected roots or embryonic-axes nuclei. Three types of protein phosphorylations were observed in nodule nuclei: Ca(2+)- and calmodulin-independent, Ca(2+)- and calmodulin-dependent, and Ca(2+)-dependent but calmodulin-independent. In addition, Ca(2+)-dependent dephosphorylation of some nuclear proteins was observed in nodule nuclei. The first and second types of phosphorylations were also present in root nuclei, but the trifluoperazine-insensitive and Ca(2+)-dependent phosphorylation (indicating calmodulin independence) occurs only in nodules. The latter appears to phosphorylate a nodule-specific protein of 65 kilodaltons and this protein was purified from other nuclear phosphorylated proteins. In addition, some nuclear proteins from uninfected tissue were found to be phosphorylated or dephosphorylated by kinases or phosphatases that originated from the nodule nuclei. These data suggest that some activities of nuclear factors in nodules may be regulated by specific phosphorylation or dephosphorylation during symbiotic interactions with rhizobia.
authors.affiliation: Department of Molecular Genetics, Ohio State University, Columbus 43210-1002.
authors.forename: H
authors.initials: H
authors.lastname: Suzuki
authors.forename: D P
authors.initials: DP
authors.lastname: Verma
mesh_terms: D002118:Calcium / Q000378:metabolism; D002147:Calmodulin / Q000378:metabolism; D002467:Cell Nucleus / Q000201:enzymology / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D009586:Nitrogen Fixation; D009687:Nuclear Proteins / Q000302:isolation & purification / Q000378:metabolism*; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D012230:Rhizobiaceae / Q000502:physiology*; D013025:Glycine max / Q000196:embryology / Q000378:metabolism* / Q000382:microbiology
chemical_list: D002147:Calmodulin; D009687:Nuclear Proteins; D011494:Protein Kinases; D002118:Calcium