title: Avian erythroblastosis virus transforms a novel mast cell-basophil precursor target in the Japanese quail.
abstract: Hematopoietic cells of the Japanese quail were transformed by avian erythroblastosis virus in vivo and in vitro. In both circumstances, the infected hematopoietic tissues exhibited a dual oncogenic response of erythroid and mast cell-basophil elements. The erythroid transformants escaped the avian erythroblastosis virus block in differentiation and progressed to hemoglobinization. Resulting basophilic cells were morphologically, biochemically, and ultrastructurally identical to mast cell-basophils observed in other species. None of the virally transformed cells actively produced reverse transcriptase activity. Nonproducer cell lines synthesized viral RNA and both v-erbA and v-erbB proteins. These results indicate that the Japanese quail has a viral target cell different from that of the chicken. The implications of a single bipotential transformation target yielding both erythroid and mast cell-basophil colonies is discussed.
authors.affiliation: Department of Pathology, University of Florida, Gainesville 32610.
authors.forename: M G
authors.initials: MG
authors.lastname: Moscovici
authors.forename: M L
authors.initials: ML
authors.lastname: Siegel
authors.forename: C
authors.initials: C
authors.lastname: Moscovici
mesh_terms: D001355:Alpharetrovirus / Q000472:pathogenicity*; D000818:Animals; D001354:Avian Leukosis Virus / Q000472:pathogenicity*; D001491:Basophils / Q000382:microbiology* / Q000473:pathology; D002472:Cell Transformation, Viral; D003370:Coturnix; D006412:Hematopoietic Stem Cells / Q000382:microbiology* / Q000473:pathology; D008407:Mast Cells / Q000382:microbiology* / Q000473:pathology; D008854:Microscopy, Electron; D011233:Precipitin Tests; D014764:Viral Proteins / Q000032:analysis; D014779:Virus Replication
chemical_list: D014764:Viral Proteins