Limited temperature-sensitive transactivation by mutant adenovirus type 2 E1a proteins.
A series of linker-scanning and deletion mutations was generated in the transactivating domain of the larger, 289-amino-acid-residue E1a protein of adenovirus type 2. Mutant genes were recombined into virus to assay the ability of the variant E1a proteins to activate expression of an E1a-dependent viral gene during infection. Results of assays performed at 32, 37, and 40 degrees C indicated that at least 2 of the 10 mutants tested showed limited temperature sensitivity for transactivation.
Fahnestock
M L
ML
Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
Lewis
J B
JB
D017456:Adenovirus Early Proteins; D000595:Amino Acid Sequence; D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins / Q000235:genetics*; D005786:Gene Expression Regulation; D006367:HeLa Cells; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D013329:Structure-Activity Relationship; D013696:Temperature; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics*
D017456:Adenovirus Early Proteins; D004268:DNA-Binding Proteins; D009856:Oncogene Proteins, Viral; D014157:Transcription Factors