title: Binding of epidermal growth factor and insulin-like growth-factor I in renal carcinoma and adjacent normal kidney tissue.
abstract: The binding of epidermal growth factor (EGF) and insulin-like growth factor I (IGF-I) to cell membranes was determined in 14 renal cancers and in 13 normal kidney tissues adjacent to the tumors. The soluble 34K IGF binding protein (34K IGF-BP) content and the phosphotyrosyl-protein phosphatase activity in renal cancer tissue and adjacent normal tissue were also determined. The specific EGF receptor binding in renal cancers was 12.7 +/- 2.5% (mean +/- SEM) as compared to 2.6 +/- 0.2% (mean +/- SEM) in normal tissues (p less than 0.01). Phosphotyrosyl-protein phosphatase activity in renal cancer tissue was less than half of that observed in normal renal tissue (p less than 0.01). The highest IGF-I binding was observed in 5 renal cancers although no consistent differences between IGF-I binding to tumor and normal tissues were observed. Both EGF and IGF binding to kidney tissue were higher than binding to gastro-intestinal tissue irrespective of whether normal or malignant tissues were compared. All normal kidney tissues and 7 of 8 kidney tumors contained measurable amounts of 34K IGF-BP as determined by RIA and the cross-linking technique. In 2 tumor tissue samples the 34K IGF-BP content was increased 8- and 15-fold over that seen in adjacent normal kidney tissue, whereas in the 6 other renal cancers the 34K IGF-BP was similar to that observed in normal kidney tissue.
authors.affiliation: Minerva Institute for Medical Research, Kauniainen, Finland.
authors.forename: F
authors.initials: F
authors.lastname: Pekonen
authors.forename: S
authors.initials: S
authors.lastname: Partanen
authors.forename: E M
authors.initials: EM
authors.lastname: Rutanen
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism*; D000368:Aged; D015179:Colorectal Neoplasms / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000378:metabolism*; D066246:ErbB Receptors / Q000378:metabolism; D005260:Female; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism*; D007680:Kidney Neoplasms / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010749:Phosphoprotein Phosphatases / Q000378:metabolism; D011485:Protein Binding; D017027:Protein Tyrosine Phosphatases; D011956:Receptors, Cell Surface / Q000378:metabolism; D017451:Receptors, Somatomedin; D012995:Solubility; D013002:Somatomedins / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000378:metabolism
chemical_list: D011956:Receptors, Cell Surface; D017451:Receptors, Somatomedin; D013002:Somatomedins; D004815:Epidermal Growth Factor; D007334:Insulin-Like Growth Factor I; D066246:ErbB Receptors; D010749:Phosphoprotein Phosphatases; D017027:Protein Tyrosine Phosphatases