Concentration of dilute solutions of macromolecules by electrophoresis.
An electrophoresis apparatus which is used for concentrating micrograms of macromolecules from solutions as large as 250 ml is described. The recoveries were greater than 91% with three different macromolecules tested (28 to 360 kDa). Solutions with volumes in the range of 35 ml were concentrated 70-fold in less than 90 min to a final volume of 0.5 ml. Larger volumes in the range of 250 ml were concentrated 227-fold in 16 h to a final volume of 1.1 ml. Sterile concentrates can be obtained if the apparatus is constructed under sterile conditions.
Atmeh
R F
RF
Department of Allied Health Sciences, University of Science and Technology, Irbid, Jordan.
D000818:Animals; D016632:Apolipoprotein A-I; D001054:Apolipoproteins A / Q000032:analysis*; D002417:Cattle; D002627:Chemistry, Physical / Q000379:methods; D004586:Electrophoresis / Q000295:instrumentation*; D005069:Evaluation Studies as Topic; D006801:Humans; D008075:Lipoproteins, HDL / Q000032:analysis*; D046911:Macromolecular Substances; D012710:Serum Albumin, Bovine / Q000032:analysis*; D012996:Solutions
D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D008075:Lipoproteins, HDL; D046911:Macromolecular Substances; D012996:Solutions; D012710:Serum Albumin, Bovine