In situ hybridization histochemistry localization of interleukin-3 mRNA in mouse brain.
The hematopoietic growth factor interleukin-3 (IL-3) promotes the proliferation and maturation of pluripotent myeloid progenitor cells. In the immune system, IL-3 is synthesized by mitogen or antigen-stimulated T lymphocytes. We demonstrate the expression of IL-3 mRNA in mouse brain by in situ hybridization histochemistry and Northern blot analysis. The IL-3 mRNA is localized in discrete areas of the brain and can be found in neuronal cell body and astrocytes. Northern analysis of cerebellar RNA, compared with mRNA extracted from WEHI-3 cells, showed a single hybridization band, approximately 1.2 kb, suggesting similar processing between brain and myeloid cells. The molecular evidence and previous observations of IL-3-like biologic activity found in the brain suggest a potential role for IL-3 in the neurobiology of the CNS.
Farrar
W L
WL
Laboratory of Molecular Immunoregulation, National Cancer Institute, Frederick, MD 21701-1013.
Vinocour
M
M
Hill
J M
JM
D000818:Animals; D001345:Autoradiography; D015152:Blotting, Northern; D001923:Brain Chemistry; D002560:Cerebrovascular Circulation; D006651:Histocytochemistry; D007375:Interleukin-1 / Q000378:metabolism / Q000502:physiology; D007377:Interleukin-3 / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D051379:Mice; D009457:Neuroglia / Q000032:analysis; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000032:analysis; D017472:Receptors, Interleukin-1
D007375:Interleukin-1; D007377:Interleukin-3; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D017472:Receptors, Interleukin-1