Primary structure of a mouse mastocytoma proteoglycan core protein.
The complete nucleotide sequence of a mouse mastocytoma proteoglycan core protein mRNA was determined. The mRNA, estimated to contain 1.1 kb, encodes a protein with an Mr of 16715. A 21-amino acid-residue region of the protein is composed of alternating serine and glycine residues. Southern-blot analysis of mouse genomic DNA with cDNA containing sequences corresponding to the Ser-Gly repeat region revealed more than 15 gene fragments. Hybridization with a probe corresponding to the N-terminal portion of the core protein identified two fragments, and cDNA covering the C-terminal part of the core protein and the 3' untranslated part of the mRNA hybridized to a single fragment. Antibodies against the core protein, obtained after immunization of rabbits with a fusion protein, reacted with both chondroitin sulphate proteoglycans and heparin proteoglycans produced by the tumour. In immunoblotting of a microsomal fraction from the mastocytoma, the antiserum recognized a single protein (Mr 17,000), which probably represents the core protein before glycosylation.
Kjellén
L
L
Department of Veterinary Medical Chemistry, Swedish University of Agricultural Sciences, Uppsala.
Pettersson
I
I
Lillhager
P
P
Steen
M L
ML
Pettersson
U
U
Lehtonen
P
P
Karlsson
T
T
Ruoslahti
E
E
Hellman
L
L
D053674:Aggrecans; D000595:Amino Acid Sequence; D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins / Q000235:genetics*; D037181:Lectins, C-Type; D012515:Mast-Cell Sarcoma / Q000235:genetics*; D051379:Mice; D008861:Microsomes / Q000378:metabolism; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D011487:Protein Conformation; D011509:Proteoglycans; D012333:RNA, Messenger / Q000235:genetics*
C508320:Acan protein, mouse; D053674:Aggrecans; D015342:DNA Probes; D004273:DNA, Neoplasm; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D037181:Lectins, C-Type; D009363:Neoplasm Proteins; D011509:Proteoglycans; D012333:RNA, Messenger; D004247:DNA