title: Alveolar macrophage elimination in vivo is associated with an increase in pulmonary immune response in mice.
abstract: A single intracheal dose of liposome-encapsuled dichloro-methylene-diphosphonate resulted in the elimination of alveolar macrophages (AM) from the lung, creating a model to study the in vivo role of AM in the pulmonary immune response. Using intratracheally administered trinitrophenyl-keyhole limpet hemocyanin (TNP-KLH), the kinetics of the response, the location and number of TNP-specific antibody-forming cells, and the different Ig classes of the antibodies produced were studied in AM-depleted animals. The results show that AM elimination has a dramatic effect on the pulmonary immune responses against TNP-KLH. An increase in APC in lung-associated lymph nodes and a prolongation of the response is found, as well as an introduction of APC in lung tissue. In both experimental groups, the majority of the TNP-specific antibodies produced was IgG, followed by IgA and IgE, while very few IgM antibodies could be detected. We conclude from these results that AM are likely to play a role in controlling the pulmonary immune response in a suppressive way, thereby limiting the possible damage caused by severe immune responses in lung tissue.
authors.affiliation: Department of Histology, Vrije Universiteit, Amsterdam, The Netherlands.
authors.forename: T
authors.initials: T
authors.lastname: Thepen
authors.forename: N
authors.initials: N
authors.lastname: Van Rooijen
authors.forename: G
authors.initials: G
authors.lastname: Kraal
mesh_terms: D000818:Animals; D000917:Antibody Formation; D004002:Clodronic Acid / Q000008:administration & dosage; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D011650:Pulmonary Alveoli / Q000166:cytology / Q000276:immunology*
chemical_list: D004002:Clodronic Acid