Urinary creatine as a possible marker for testicular damage: studies with the testicular toxic compound 2-methoxyethanol.
A single dose of 500 mg/kg of 2-methoxyethanol given to male rats caused testicular damage, detected by histopathology, a significant reduction in relative testis weight, and a significant rise in urinary creatine, maximal at 48 h after dosing. Further studies showed that the reduction in relative testis weight and creatine excretion were dose related. Conversely, urinary creatinine showed a dose-related decrease at 24 h after dosing. In female rats dosed with 500 mg/kg of 2-methoxyethanol, there was no increase in urinary creatine or decrease in creatinine. These data suggest that urinary creatine may be a useful, noninvasive marker of testicular damage.
Rawcliffe
L
L
Toxicology Unit, School of Pharmacy, London.
Creasy
D
D
Timbrell
J A
JA
D000818:Animals; D015415:Biomarkers; D003401:Creatine / Q000652:urine*; D003404:Creatinine / Q000652:urine; D005026:Ethylene Glycols / Q000633:toxicity*; D005260:Female; D008297:Male; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D013733:Testicular Diseases / Q000139:chemically induced / Q000652:urine*; D013997:Time Factors
D015415:Biomarkers; D005026:Ethylene Glycols; D003404:Creatinine; C005219:methyl cellosolve; D003401:Creatine