Crystallization and preliminary X-ray analysis of porin from Rhodobacter capsulatus.
Porin monomers of the phototrophic bacterium Rhodobacter capsulatus were purified. Crystals were obtained from a solution of porin solubilized with the detergent octyltetraoxyethylene within 5 days using the vapor phase equilibration technique. The crystals were rhombohedral with an edge length of 0.4 mm. The space group was trigonal R3 with unit cell constants of a = b = 95 A, c = 147 A. Reflexions were observed to 3.2 A.
Nestel
U
U
Institut für Biophysik und Strahlenbiologie der Albert-Ludwigs-Universität, Freiburg i.Br., FRG.
Wacker
T
T
Woitzik
D
D
Weckesser
J
J
Kreutz
W
W
Welte
W
W
D001425:Bacterial Outer Membrane Proteins / Q000648:ultrastructure*; D003461:Crystallography; D006090:Gram-Negative Bacteria / Q000032:analysis; D008970:Molecular Weight; D018272:Porins; D011487:Protein Conformation; D014961:X-Ray Diffraction
D001425:Bacterial Outer Membrane Proteins; D018272:Porins