title: Reactivities of polyclonal and monoclonal antibodies raised to the major capsid protein of human papillomavirus type 16.
abstract: Polyclonal and monoclonal antibodies have been raised against a fusion protein containing beta-galactosidase and part of the major capsid protein L1 of the human papillomavirus (HPV) type 16. The polyclonal antibodies cross-reacted with the L1 protein of several HPV types including HPV-1, -2, -6 and -11 when reacted with virus-infected tissue sections, and with HPV-6 and -18 L1 fusion proteins on Western blotting. Monoclonal antibodies against the L1 fusion protein of HPV-16 reacted only with HPV-16 L1 fusion proteins on Western blots and with HPV-16-containing biopsy sections as assessed by in situ DNA-DNA hybridization. These antibodies did not detect HPV-6 L1 protein after Western blotting or in HPV-6-infected tissue sections, although one did react with an HPV-18 fusion protein after Western blotting. The monoclonal antibodies were able to detect HPV-16 antigens in routine formaldehyde-fixed, wax-embedded sections of cervical intraepithelial neoplasia sections. HPV-16 L1 proteins were seen in one-third of biopsies that were positive using the polyclonal cross-reacting antisera. Polyclonal antibodies to fusion proteins containing part of the minor capsid protein L2 of HPV-6 or -16 appeared to be more type-specific as no cross-reactivity was seen when these antibodies were reacted with HPV-1- and -2-infected tissue sections.
authors.affiliation: Department of Microbiology, United Medical School Guy's Hospital, London, U.K.
authors.forename: D
authors.initials: D
authors.lastname: Patel
authors.forename: P S
authors.initials: PS
authors.lastname: Shepherd
authors.forename: J A
authors.initials: JA
authors.lastname: Naylor
authors.forename: D J
authors.initials: DJ
authors.lastname: McCance
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D000918:Antibody Specificity; D015153:Blotting, Western; D002213:Capsid / Q000276:immunology*; D003218:Condylomata Acuminata / Q000276:immunology / Q000382:microbiology; D003429:Cross Reactions; D015344:DNA Probes, HPV; D005260:Female; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D051379:Mice; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000276:immunology*; D011817:Rabbits; D002583:Uterine Cervical Neoplasms / Q000276:immunology / Q000382:microbiology; D014760:Viral Fusion Proteins / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D015344:DNA Probes, HPV; D007106:Immune Sera; D014760:Viral Fusion Proteins