Free gp70 from FeLV: enrichment from cell culture fluid by ferric oxide-agarose chromatography.
A new chromatographic material based on beads of macroporous crosslinked agarose containing ferric oxide particles was used for enrichment of gp70--the envelope glycoprotein of feline leukemia virus (FeLV). Free gp70 was purified from cell culture fluid in one step with a recovery of 50 to 60% and a purification of about 60 times. The described procedure is a suitable first step for the purification of gp70 from large volumes of cell culture fluid.
Zelikman
I
I
Institute of Biochemistry, Uppsala University, Sweden.
Akerblom
L
L
Hjertèn
S
S
Morein
B
B
D000818:Animals; D002415:Cats; D002847:Chromatography, Agarose; D003470:Culture Media; D005290:Ferric Compounds; D015151:Immunoblotting; D007525:Isoelectric Focusing; D005256:Leukemia Virus, Feline / Q000032:analysis*; D012191:Retroviridae Proteins / Q000302:isolation & purification*; D015682:Retroviridae Proteins, Oncogenic; D014407:Tumor Cells, Cultured / Q000032:analysis; D014759:Viral Envelope Proteins / Q000302:isolation & purification*
D003470:Culture Media; D005290:Ferric Compounds; D012191:Retroviridae Proteins; D015682:Retroviridae Proteins, Oncogenic; D014759:Viral Envelope Proteins; C000499:ferric oxide