Expression of low-affinity receptor for IgE (Fc epsilon RII, CD23) and IgE-BF (soluble CD23) release by lymphoblastoid B-cell line RPMI-8866 and human peripheral lymphocytes of normal and atopic donors.
The low-affinity receptor for IgE (CD23) as well as the soluble IgE-binding factors (IgE-BF, sCD23) are important factors in IgE antibody regulation. The CD23 expression and the concomitant release of CD23 were analysed from the lymphoblastoid B-cell line RPMI-8866 and from peripheral blood lymphocytes (PBL) of healthy volunteers as well as atopic patients. CD23 expression and sCD23 release of RPMI-8866 cells were dependent on the stage of culture. While CD23 expression decreased with increasing time of culture (Day 1-3), the sCD23 release was enhanced during the culture period. Cytokines such as IL-4, IL-2, TNF alpha and IFN-gamma exerted various effects on the target cells depending on the culture period. CD23 expression on normal lymphocytes was lower compared with the expression on atopic cells. Lymphokines (IL-2, IL-4) as well as mitogens (PHA, Con A) enhanced CD23 expression and IgE-BF (sCD23) release. The degree of enhancement was always higher with atopic cells compared with the results obtained with cells of normal donors.
Bujanowski-Weber
J
J
Institut für Medizinische, Mikrobiologie and Immunologie, Arbeitsgruppe für Infektabwehremechanismen, Ruhr-Universität Bochum, FGR.
Brings
B
B
Knöller
I
I
Pfeil
T
T
König
W
W
D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis*; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D001685:Biological Factors / Q000494:pharmacology; D002460:Cell Line; D006224:Cricetinae; D016207:Cytokines; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology*; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000378:metabolism* / Q000494:pharmacology; D029584:Prostatic Secretory Proteins; D011961:Receptors, Fc / Q000032:analysis*; D017455:Receptors, IgE; D013491:Suppressor Factors, Immunologic / Q000378:metabolism*
D000944:Antigens, Differentiation, B-Lymphocyte; D001685:Biological Factors; D016207:Cytokines; D008222:Lymphokines; D029584:Prostatic Secretory Proteins; D011961:Receptors, Fc; D017455:Receptors, IgE; D013491:Suppressor Factors, Immunologic; C043076:beta-microseminoprotein; C013059:immunoglobulin-binding factors; D007073:Immunoglobulin E