[Effect of anticoagulants on the activity of trypsin-like proteinases in human blood plasma].
Influence of some anticoagulants (heparin, sodium citrate, their mixture) on blood trypsin-like proteinases activity was examined. The activity was determined using synthetic substrate N-alpha-benzoyl-L-arginine-p-nitroanilide. It was shown that heparin greatly activated blood trypsin-like proteinases (at heparin concentration 5 unit/ml of blood, the enzyme activity in plasma was about 10 times higher than the activity in serum). Heparin added to serum caused the activation effect too, maximum of activation was reached at heparin concentration in serum 800 unit/ml, following increase of heparin concentration did not led to the activity change. Sodium citrate had no significant effect both on the trypsin-like proteinases activity and on the activation effect of heparin. It was found that investigated anticoagulants did not affect blood antitryptic activity.
Babiĭ
A V
AV
Sakharov
I Iu
IIu
Pokrovskiĭ
S N
SN
D000328:Adult; D000925:Anticoagulants / Q000494:pharmacology*; D001586:Benzoylarginine Nitroanilide / Q000097:blood; D002951:Citrates / Q000494:pharmacology; D019343:Citric Acid; D004305:Dose-Response Relationship, Drug; D010450:Endopeptidases / Q000097:blood*; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D013379:Substrate Specificity / Q000187:drug effects; D014357:Trypsin / Q000097:blood*; D014361:Trypsin Inhibitors / Q000097:blood
D000925:Anticoagulants; D002951:Citrates; D014361:Trypsin Inhibitors; D019343:Citric Acid; D006493:Heparin; D001586:Benzoylarginine Nitroanilide; D010450:Endopeptidases; D014357:Trypsin
Vliianie antikoagulantov na aktivnost' tripsinopodobnykh proteinaz plazmy krovi cheloveka.