title: Differential response of bovine T-cell lines to membrane and soluble antigens of Theileria parva schizont-infected cells.
abstract: T-cell lines generated from Theileria parva-immune cattle were used to identify antigens associated with schizont-infected lymphoblastoid cells. Homogenates prepared from T. parva-infected cells were fractionated by differential centrifugation, and antigenically distinct soluble and membrane-bound antigens were detected by the differential stimulation of cell lines derived from two animals. Activity in the soluble fraction was not attributable to either a mitogen or interleukin 2. Activity in the membrane fraction was associated with schizont membranes as indicated by the presence in this fraction of a parasite protein detected by immunoblot analysis using a schizont-specific monoclonal antibody. Elimination of intracellular schizonts over time, using the anti-theilerial drug, parvaquone, resulted in a concomitant loss of antigenicity in infected cells and in subcellular fractions prepared from drug-treated cells, demonstrating that stimulation of Theileria-specific helper and cytotoxic T-cell responses is associated with the presence of the parasite.
authors.affiliation: International Laboratory for Research on Animal Diseases (ILRAD), Nairobi, Kenya.
authors.forename: W C
authors.initials: WC
authors.lastname: Brown
authors.forename: C
authors.initials: C
authors.lastname: Sugimoto
authors.forename: P A
authors.initials: PA
authors.lastname: Conrad
authors.forename: D J
authors.initials: DJ
authors.lastname: Grab
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D016782:Apicomplexa / Q000276:immunology*; D002417:Cattle; D002458:Cell Fractionation; D002460:Cell Line; D015151:Immunoblotting; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology / Q000469:parasitology; D010641:Phenotype; D012995:Solubility; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D013801:Theileriasis / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D000954:Antigens, Surface