Enzymic synthesis, chemical characterisation and Sda activity of GalNAc beta 1-4[NeuAc alpha 2-3]Gal beta 1-4GlcNAc and GalNAc beta 1-4[NeuAc alpha 2-3]Gal beta 1-4Glc.
The tetrasaccharides GalNAc beta 1-4[NeuAc alpha 2-3]Gal beta 1-4Glc and GalNAc beta 1-4[NeuAc alpha 2-3]Gal beta 1-4GlcNAc were synthesised by enzymic transfer of GalNAc from UDP-GalNAc to 3'-sialyllactose (NeuAc alpha 2-3 Gal beta 1-4Glc) and 3'-sialyl-N-acetyllactosamine (NeuAc alpha 2-3Gal beta 1-4GlcNAc). The structures of the products were established by methylation and 1H-500 MHz NMR spectroscopy. In Sda serological tests the product formed with 3'-sialyl-N-acetyllactosamine was highly active whereas that formed with 3'-sialyllactose had only weak activity.
Soh
C P
CP
Division of Immunochemical Genetics, MRC Clinical Research Centre, Harrow, Middlesex, U.K.
Donald
A S
AS
Feeney
J
J
Morgan
W T
WT
Watkins
W M
WM
D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D005700:Galactosyltransferases / Q000378:metabolism; D006168:Guinea Pigs; D006384:Hemagglutination; D006649:Histocompatibility Antigens; D006801:Humans; D006859:Hydrogen; D007668:Kidney / Q000201:enzymology; D009682:Magnetic Resonance Spectroscopy; D008285:Major Histocompatibility Complex; D008745:Methylation; D008969:Molecular Sequence Data; D017350:N-Acetylgalactosaminyltransferases; D009844:Oligosaccharides / Q000138:chemical synthesis / Q000737:chemistry* / Q000652:urine
D006649:Histocompatibility Antigens; D009844:Oligosaccharides; D006859:Hydrogen; D005700:Galactosyltransferases; D017350:N-Acetylgalactosaminyltransferases; C003742:UDPgalactosamine-galactose acetylgalactosaminyltransferase