Ability of pure resting CD8+ human T cells to respond to alloantigen.
The ability of highly purified resting human CD8 cells to respond to alloantigens in vitro was examined. Necessary conditions for induction of interleukin 2 receptors (IL-2R), IL-2 production, proliferative responses, and various effector functions were determined. Allogeneic non-T cells induced IL-2R expression in a high proportion of resting CD4 and CD8 cells, but only CD4 cells produced detectable amounts of IL-2. CD8 cells also became IL-2 responsive upon stimulation with purified resting allogeneic CD4 or CD8 cells, indicating that HLA class I+, II- cells alone may initiate activation of resting CD8 cells. The activated CD8 cells needed the presence of simultaneously activated CD4 cells or exogenous IL-2 to be able to synthesize DNA. Effector functions like cytotoxicity, mixed lymphocyte culture (MLC) suppression, or gamma interferon (IFN-gamma) production were also only detectable when the CD8 cells were activated in the presence of IL-2.
Leivestad
T
T
Institute of Transplantation Immunology, National Hospital, Oslo, Norway.
Halvorsen
R
R
Gaudernack
G
G
Thorsby
E
E
D000945:Antigens, Differentiation, T-Lymphocyte; D016827:CD8 Antigens; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis / Q000502:physiology; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D010641:Phenotype; D015375:Receptors, Interleukin-2 / Q000378:metabolism; D015290:Second Messenger Systems; D013601:T-Lymphocytes / Q000145:classification* / Q000276:immunology / Q000378:metabolism; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D000945:Antigens, Differentiation, T-Lymphocyte; D016827:CD8 Antigens; D006680:HLA Antigens; D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D007371:Interferon-gamma