Specific disruption of intermediate filaments and the nuclear lamina by the 19-kDa product of the adenovirus E1B oncogene.
The 19-kDa protein encoded within the adenovirus E1B gene is essential for transformation by adenovirus and for proper regulation of viral early gene transcription. In order to investigate the biological function of the 19-kDa E1B protein, vectors were constructed to produce the 19-kDa protein in mammalian cells under the direction of heterologous promoters. Surprisingly, during transient expression, the E1B 19-kDa protein specifically associated with and disrupted the organization of intermediate filaments and the nuclear lamina, without disturbing the organization of other cytoskeletal networks. These results directly demonstrate an effect of a viral transforming protein on the cytoskeleton and suggest a role for intermediate filaments and the nuclear lamina in modulation of viral gene expression and the process of oncogenic transformation.
White
E
E
Cold Spring Harbor Laboratory, NY 11724.
Cipriani
R
R
D017456:Adenovirus Early Proteins; D000260:Adenoviruses, Human / Q000235:genetics*; D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure*; D002472:Cell Transformation, Viral; D003599:Cytoskeleton / Q000648:ultrastructure*; D004268:DNA-Binding Proteins / Q000235:genetics; D005814:Genes, Viral; D006367:HeLa Cells / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D007382:Intermediate Filaments / Q000648:ultrastructure*; D008970:Molecular Weight; D009856:Oncogene Proteins, Viral / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D009857:Oncogenes; D010957:Plasmids; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014162:Transfection; D015678:Viral Structural Proteins / Q000235:genetics
D017456:Adenovirus Early Proteins; D004268:DNA-Binding Proteins; D009856:Oncogene Proteins, Viral; D015678:Viral Structural Proteins