Comparison of 5'-nucleotidase activities among cultured murine melanoma cell lines.
The activity of 5'-nucleotidase and ouabain-sensitive Na/K ATPase was determined in seven different mouse melanoma cell lines. Ouabain-sensitive Na/K ATPase activity was found in NP40-treated cell homogenates of all cell lines. However, 5'-nucleotidase activity was found in only one mouse melanoma cell line--JB/RH. The absence of expression of 5'-nucleotidase activity in the other six cell lines is not associated with pigmentation in melanoma cells, nor is the gene switched off in all transformed melanocytes of C57BL/6 origin.
Moody
D
D
Department of Pathology, Georgetown University Medical Center, Washington, D.C. 20007.
Williams
L J
LJ
Gersten
D M
DM
D015720:5'-Nucleotidase; D000818:Animals; D002460:Cell Line; D008546:Melanoma, Experimental / Q000201:enzymology*; D051379:Mice; D009708:Nucleotidases / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D009708:Nucleotidases; D015720:5'-Nucleotidase