[The effect of the elastase on aminonucleoside nephrosis].
In this paper, we studied the effect of elastase on aminonucleoside (AN) nephrosis which is considered a model of focal glomerular sclerosis (FGS). Elastase is an enzyme which disintegrates elastin, discovered by Balo, and used in the treatment of arteriosclerosis and hyperlipidemia. It has also been known to improve metabolism of acid mucopolysaccharides, so, this study focused on metabolic improvement. Three groups of male Sprague-Dawley rats were studied and observed at regular intervals; 30, 60, 90 days. The ANE group (AN + elastase) was administered one shot of AN (10 mg/100 g B.W.) during the test interval, while elastase (5 mg/kg B.W.) was injected 5 days/week for the entire test interval. The AN group was administered one shot of AN only. The third group was a control (C). The following results were observed: (1) Focal segmental hyalinosis and sclerosis (FSHS); ANE group was weaker than AN group. (2) Other significant qualifying glomerular changes (vacuolar change and hyaline droplets of the epithelial cells, adhesion, and foam cells); ANE group was weaker than AN group. (3) Anion loss in GBM was shown by a lack of colloidal iron staining under light microscopy, and by a lack of PEI particles under electron microscopy; there was significantly less anion loss with ANE group, than with AN group. The findings suggest that elastase has an affect on the metabolism of acid mucopolysaccharide and collagen in sclerotic lesion, and may restrain the progress of amino-nucleoside nephrosis.
Hirabayashi
K
K
Ishitobi
F
F
Ueda
Y
Y
Takimoto
T
T
Mizoguchi
K
K
Kure
N
N
Ono
H
H
Oka
K
K
Iidaka
K
K
D000818:Animals; D003094:Collagen / Q000378:metabolism; D005921:Glomerulonephritis / Q000188:drug therapy*; D005923:Glomerulosclerosis, Focal Segmental / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism; D008297:Male; D010196:Pancreatic Elastase / Q000627:therapeutic use*; D011691:Puromycin / Q000031:analogs & derivatives; D011692:Puromycin Aminonucleoside; D051381:Rats; D011919:Rats, Inbred Strains
D006025:Glycosaminoglycans; D011691:Puromycin; D011692:Puromycin Aminonucleoside; D003094:Collagen; D010196:Pancreatic Elastase