Comparative investigation on the extractibility of acetylcholinesterase from normal and dystrophic muscles.
Following combined administration of nonionic detergent and collagenase considerable differences were observed in extractibility of acetylcholinesterase activity from normal and from dystrophic rabbit muscles. The results obtained in the experiments carried out on vitamin-E deficient rats corroborated the authors' previous experiences obtained in vitamin-E deficient rabbits. However, as to the effect of the detergent, considerable differences were observed between the two species. If one compares the activities referred to 1 g muscle or to the total tissue mass it is clear that primarily the concentration of acetylcholinesterase was increased. The activity measured in the presence of butyrylthiocholine increased only to small extent in vitamin-E deficient dystrophy.
Katona
G
G
2nd Institute of Biochemistry, Semmelweis University Medical School, Budapest, Hungary.
Székessy-Hermann
V
V
D000110:Acetylcholinesterase / Q000302:isolation & purification*; D000818:Animals; D008297:Male; D003012:Microbial Collagenase / Q000494:pharmacology; D009132:Muscles / Q000201:enzymology*; D009137:Muscular Dystrophy, Animal / Q000201:enzymology*; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D014811:Vitamin E Deficiency / Q000201:enzymology
D011092:Polyethylene Glycols; D017830:Octoxynol; D000110:Acetylcholinesterase; D003012:Microbial Collagenase