title: Measurement of urinary leukotrienes by reversed-phase liquid chromatography and radioimmunoassay.
abstract: Leukotriene (LT) E4, an important LT metabolite appearing in urine, can be rapidly separated from normal and pathological urines by automated reversed-phase HPLC after a simple sample-processing. The recoveries of LTE4 afforded by this system (86.4 +/- 6.5%, mean +/- SEM for 60 ng/L, 85.4 +/- 0.3% for 200 ng/L) are superior to those obtained by a manual extraction method. Consistency of results is similar. Highly reproducible retention times combined with a radioimmunoassay allow one to identify (based on co-elution) and quantify as little as 8 ng/L LTE4 in a 10-mL urine sample. LTE4 concentrations in urine from healthy persons approach this value (17 +/- 5 ng/L), whereas samples from patients with cardiac ischemia show a wider range of concentrations (8 to 388 ng/L), up to 50 times the detection limit. Thus this method is applicable to the noninvasive investigation of leukotriene involvement in a wide range of ischemic, inflammatory, and hypersensitive conditions.
authors.affiliation: Merck Frosst Centre for Therapeutic Research, Department of Pharmacology, Pointe Claire-Dorval, Quebec, Canada.
authors.forename: P
authors.initials: P
authors.lastname: Tagari
authors.forename: D
authors.initials: D
authors.lastname: Ethier
authors.forename: M
authors.initials: M
authors.lastname: Carry
authors.forename: V
authors.initials: V
authors.lastname: Korley
authors.forename: S
authors.initials: S
authors.lastname: Charleson
authors.forename: Y
authors.initials: Y
authors.lastname: Girard
authors.forename: R
authors.initials: R
authors.lastname: Zamboni
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003327:Coronary Disease / Q000652:urine*; D006801:Humans; D017999:Leukotriene E4; D013189:SRS-A / Q000031:analogs & derivatives* / Q000652:urine*
chemical_list: D013189:SRS-A; D017999:Leukotriene E4