title: The use of human follicular fluid in gamete intra-fallopian transfer.
abstract: We undertook a prospective study to compare our gamete intra-Fallopian transfer (GIFT) procedure with or without the use of human follicular fluid (FF) as a constituent for the final spermatozoal suspension and as the tubal transfer medium for both eggs and spermatozoa. We routinely perform an intrauterine and intracervical insemination (IUI and ICI) following GIFT, and FF or culture medium was used accordingly as a constituent in this spermatozoal suspension also. When FF was used (26 cycles), clear FF taken from the first egg-bearing follicle was sterilized by micropore filtration, gassed with 5% CO2 in air and warmed to 37 degrees C. This FF was then used to dilute the spermatozoal suspension (50:50, v/v) for both tubal, uterine and cervical inseminations at least 30 min before transfer, and all transferable eggs were placed into this FF before transfer. Alternatively (30 control cycles), eggs and spermatozoa were prepared and transferred in Earle's medium supplemented with 10% pooled fetal cord serum. The FF and control patient groups were relatively homogeneous, with no statistically significant differences in ovarian response, oocyte retrieval or transfer or seminal profiles. The outcome of the GIFT procedures using FF or culture medium showed no significant advantage of the use of FF. The clinical pregnancy rate was similar in both groups: 50% (15/30) control; 46.2% (12/26) FF.
authors.affiliation: IVF Centre, Hong Kong Sanatorium and Hospital, Happy Valley, Hong Kong.
authors.forename: M J
authors.initials: MJ
authors.lastname: Tucker
authors.forename: Y M
authors.initials: YM
authors.lastname: Chan
authors.forename: S Y
authors.initials: SY
authors.lastname: Chan
authors.forename: C J
authors.initials: CJ
authors.lastname: Wong
authors.forename: K R
authors.initials: KR
authors.lastname: Mao
authors.forename: M K
authors.initials: MK
authors.lastname: Leong
authors.forename: C K
authors.initials: CK
authors.lastname: Leung
mesh_terms: D000328:Adult; D006063:Chorionic Gonadotropin / Q000627:therapeutic use; D003584:Cytological Techniques; D005260:Female; D005640:Follicle Stimulating Hormone / Q000627:therapeutic use; D015571:Follicular Fluid / Q000502:physiology*; D015181:Gamete Intrafallopian Transfer; D006801:Humans; D007247:Infertility, Female / Q000628:therapy; D008297:Male; D008596:Menotropins / Q000627:therapeutic use; D011247:Pregnancy; D012661:Semen / Q000166:cytology / Q000502:physiology
chemical_list: D006063:Chorionic Gonadotropin; D008596:Menotropins; D005640:Follicle Stimulating Hormone