Effects of tetradecyl sulfate on electrophoretic resolution of kidney Na,K-ATPase catalytic subunit isoforms.
Inclusion of sodium tetradecyl sulfate in the Laemmli polyacrylamide gel electrophoresis system produces resolution of the kidney Na,K-ATPase catalytic subunit into a doublet and sharpens demarcation of catalytic subunit isoforms in NA,K-ATPase from brain.
Siegel
G J
GJ
Department of Neurology, University of Michigan Medical Center, Ann Arbor 48109.
Desmond
T J
TJ
D000818:Animals; D001921:Brain / Q000201:enzymology; D002138:Calibration; D002384:Catalysis; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel / Q000592:standards; D005233:Fatty Alcohols; D007527:Isoenzymes / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology*; D051379:Mice; D012967:Sodium Dodecyl Sulfate; D012981:Sodium Tetradecyl Sulfate; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism* / Q000592:standards
D005233:Fatty Alcohols; D007527:Isoenzymes; D012967:Sodium Dodecyl Sulfate; D000254:Sodium-Potassium-Exchanging ATPase; D012981:Sodium Tetradecyl Sulfate