title: Defective virus is associated with induction of murine retrovirus-induced immunodeficiency syndrome.
abstract: C57BL/6 mice infected with a mixture of murine leukemia viruses (MuLV) develop a syndrome characterized by lymphoproliferation and profound immunodeficiency. Analyses of this viral mixture (LP-BM5 MuLV) showed that it includes replication-competent ecotropic and mink cell focus-inducing MuLV and defective viruses with genome sizes of 3.8-6.5 kilobases. The ecotropic and mink cell focus-inducing MuLV biologically cloned from the mixture did not induce disease, whereas viral preparations containing the ecotropic MuLV and 4.8-kilobase defective virus were active. Cells producing the 4.8-kilobase defective virus expressed an unusual gag-encoded polyprotein of Mr 60,000.
authors.affiliation: Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892.
authors.forename: S K
authors.initials: SK
authors.lastname: Chattopadhyay
authors.forename: H C
authors.initials: HC
authors.lastname: Morse
authors.forename: M
authors.initials: M
authors.lastname: Makino
authors.forename: S K
authors.initials: SK
authors.lastname: Ruscetti
authors.forename: J W
authors.initials: JW
authors.lastname: Hartley
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000662:veterinary*; D000818:Animals; D004279:DNA, Viral / Q000235:genetics; D003673:Defective Viruses / Q000472:pathogenicity*; D015683:Gene Products, gag; D007153:Immunologic Deficiency Syndromes / Q000382:microbiology*; D009052:Leukemia Virus, Murine / Q000235:genetics / Q000472:pathogenicity*; D051379:Mice; D008810:Mice, Inbred C57BL; D015183:Restriction Mapping; D012191:Retroviridae Proteins / Q000235:genetics
chemical_list: D004279:DNA, Viral; D015683:Gene Products, gag; D012191:Retroviridae Proteins