title: Characterization of the CD8+CD45R+(2H4+) suppressor effector cell.
abstract: In the present study, we have investigated the molecular basis for immunoregulatory function of CD8 cells after autologous mixed lymphocyte reaction (AMLR) activation. We demonstrated that the CD8+CD45R+ but not the CD8+CD45R- subset of cells was a subpopulation, with the majority of suppressor activity after AMLR activation. In contrast, cytotoxic activity against alloantigens resided in both the CD8+CD45R+ and CD8+CD45R- subsets of cells. Importantly, the treatment of AMLR-activated CD8 cells with anti-CD45R antibody or anti-CD3 antibody abolished the suppressor function of these cells, which contrasts with earlier studies showing that treatment of AMLR activated CD4 suppressor inducer cells could be blocked with anti-CD45R but not with anti-CD3 antibody. The results suggest that the CD45R antigen as well as the CD3-T cell receptor (TCR) complex have an important role in the suppressor function of AMLR-activated cells.
authors.affiliation: Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, MA.
authors.forename: C
authors.initials: C
authors.lastname: Morimoto
authors.forename: T
authors.initials: T
authors.lastname: Takeuchi
authors.forename: S F
authors.initials: SF
authors.lastname: Schlossman
mesh_terms: D015703:Antigens, CD; D000943:Antigens, Differentiation; D000945:Antigens, Differentiation, T-Lymphocyte; D001324:Autoantigens; D017252:CD3 Complex; D016827:CD8 Antigens; D006801:Humans; D066298:In Vitro Techniques; D007519:Isoantigens; D017493:Leukocyte Common Antigens; D007959:Lymphocyte Culture Test, Mixed; D011948:Receptors, Antigen, T-Cell; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
chemical_list: D015703:Antigens, CD; D000943:Antigens, Differentiation; D000945:Antigens, Differentiation, T-Lymphocyte; D001324:Autoantigens; D017252:CD3 Complex; D016827:CD8 Antigens; D007519:Isoantigens; D011948:Receptors, Antigen, T-Cell; D017493:Leukocyte Common Antigens