title: The urine from patients with peritonitis as a rich source for purifying human acid sphingomyelinase and other lysosomal enzymes.
abstract: Milligram amounts of human acid sphingomyelinase (EC 3.1.4.12) were purified to 95% homogeneity using urine from patients with acute peritonitis. The activity of this enzyme is elevated more than 200-times in the urine of these patients. To a lesser extent, levels of some other lysosomal hydrolases are also elevated.
authors.affiliation: Institut für Organische Chemie und Biochemie, Universität Bonn, F.R.G.
authors.forename: L E
authors.initials: LE
authors.lastname: Quintern
authors.forename: T S
authors.initials: TS
authors.lastname: Zenk
authors.forename: K
authors.initials: K
authors.lastname: Sandhoff
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D005260:Female; D006801:Humans; D007525:Isoelectric Focusing; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D008875:Middle Aged; D010538:Peritonitis / Q000652:urine*; D010727:Phosphoric Diester Hydrolases / Q000302:isolation & purification*; D013108:Sphingomyelin Phosphodiesterase / Q000302:isolation & purification* / Q000652:urine
chemical_list: D010727:Phosphoric Diester Hydrolases; D013108:Sphingomyelin Phosphodiesterase