title: Isolation and characterization of iso-rANP, a new natriuretic peptide from rat atria.
abstract: Using a specific radioimmunoassay we have isolated and sequenced a new 45-amino acid peptide from rat atria which exhibits similar physiological and pharmacological properties to rat atrial natriuretic peptide (rANP). We have termed the new peptide iso-rANP, because of its functional and structural similarities to rANP. Amino acid sequence differences show that iso-rANP is genetically distinct from rANP. Iso-rANP has a single disulfide bond between residues 23-39 and this portion of the peptide shows substantial homology to rANP and to porcine brain natriuretic peptide (BNP). Little homology is evident at the N- and C-termini of iso-rANP and ANP. Iso-rANP is equipotent with rANP in eliciting diuresis, natriuresis and hypotension in the bioassay rat.
authors.affiliation: Department of Biochemistry, Queen's University, Kingston, Ontario, Canada.
authors.forename: T G
authors.initials: TG
authors.lastname: Flynn
authors.forename: A
authors.initials: A
authors.lastname: Brar
authors.forename: L
authors.initials: L
authors.lastname: Tremblay
authors.forename: I
authors.initials: I
authors.lastname: Sarda
authors.forename: C
authors.initials: C
authors.lastname: Lyons
authors.forename: D B
authors.initials: DB
authors.lastname: Jennings
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000138:chemical synthesis / Q000302:isolation & purification* / Q000494:pharmacology; D001794:Blood Pressure / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D006325:Heart Atria / Q000032:analysis*; D006339:Heart Rate / Q000187:drug effects; D008969:Molecular Sequence Data; D011863:Radioimmunoassay; D051381:Rats
chemical_list: D009320:Atrial Natriuretic Factor