title: Contribution of amino acids in protective solutions to postischemic functional recovery of canine kidneys.
abstract: Amino acids are known to increase glomerular filtration rate (GFR). There is also an early resumption of filtration following 2-h renal ischemic stress under protection by histidine-buffered histidine-tryptophan-ketoglutarate solution (HTK), possibly due in part to an amino acid effect. Hence, we have examined the possibility of further enhancing the postischemic GFR by adding 32 (ASP I; 4 mM Mg2+) or 36 (ASP II; 6 mM Mg2+) mM L-aspartate (asp) or 32 mM DL-aspartate (ASP III) to the HTK solution in place of chloride. After infusion of 500 ml 5% glucose, canine kidneys were protected by an 8-min perfusion with HTK (n = 5), ASP I (n = 4), ASP II (n = 5) or ASP III-solution (n = 3). The subsequent ischemia lasted for 2 h at 27-31 degrees C. During reperfusion, both GFR and filtration fraction (FF) were higher in kidneys protected by L-aspartate-containing solutions. ASP III showed no improvement against HTK. An additional preischemic intra-aortal application of HTK or ASP I solution just above the exit of the renal arteries prior to the intrinsic protective perfusion further raised the postischemic GFR. The present results suggest that L-aspartate but also histidine may have favorable amino acid effects in renal protective solutions in addition to known positive effects of histidine.
authors.affiliation: Department of Physiology and Pathophysiology, University of Göttingen, FRG.
authors.forename: G
authors.initials: G
authors.lastname: Kehrer
authors.forename: M
authors.initials: M
authors.lastname: Blech
authors.forename: M
authors.initials: M
authors.lastname: Kallerhoff
authors.forename: M
authors.initials: M
authors.lastname: Langheinrich
authors.forename: H J
authors.initials: HJ
authors.lastname: Bretschneider
mesh_terms: D000818:Animals; D001011:Aorta; D001224:Aspartic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D004231:Diuresis / Q000187:drug effects; D004285:Dogs; D005260:Female; D005919:Glomerular Filtration Rate / Q000187:drug effects*; D005947:Glucose / Q000008:administration & dosage; D006639:Histidine / Q000494:pharmacology*; D007511:Ischemia / Q000503:physiopathology*; D007668:Kidney / Q000098:blood supply* / Q000187:drug effects / Q000503:physiopathology; D007677:Kidney Function Tests; D008297:Male; D008353:Mannitol / Q000008:administration & dosage; D010101:Oxygen Consumption / Q000187:drug effects; D010477:Perfusion; D011189:Potassium Chloride / Q000008:administration & dosage; D011343:Procaine / Q000008:administration & dosage; D012039:Regional Blood Flow / Q000187:drug effects; D015424:Reperfusion
chemical_list: C039984:Bretschneider cardioplegic solution; D001224:Aspartic Acid; D008353:Mannitol; D006639:Histidine; D011343:Procaine; D011189:Potassium Chloride; D005947:Glucose