Reversed-phase high-performance liquid chromatographic purification of 125I-labeled rat growth hormone-releasing hormone for radioimmunoassay.
Using reversed-phase high-performance liquid chromatography, an effective purification was developed for radioiodinated rat growth hormone-releasing hormone (rGHRH) with isolation of monoiodo-rGHRH. The use of this purified radio-label resulted in improvement in binding, half-maximum displacement and sensitivity in radioimmunoassay for rGHRH. The improved radioimmunoassay performance allowed the measurement of in vitro basal and stimulated the release of rGHRH from incubated hypothalamic preparations.
Leidy
J W
JW
Huntington Veterans Administration Medical Center, WV 25704.
D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004355:Drug Stability; D013007:Growth Hormone-Releasing Hormone / Q000302:isolation & purification*; D007457:Iodine Radioisotopes; D008297:Male; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains
D007457:Iodine Radioisotopes; D013007:Growth Hormone-Releasing Hormone