title: Comparison of 5'-nucleotidase activities among cultured murine melanoma cell lines.
abstract: The activity of 5'-nucleotidase and ouabain-sensitive Na/K ATPase was determined in seven different mouse melanoma cell lines. Ouabain-sensitive Na/K ATPase activity was found in NP40-treated cell homogenates of all cell lines. However, 5'-nucleotidase activity was found in only one mouse melanoma cell line--JB/RH. The absence of expression of 5'-nucleotidase activity in the other six cell lines is not associated with pigmentation in melanoma cells, nor is the gene switched off in all transformed melanocytes of C57BL/6 origin.
authors.affiliation: Department of Pathology, Georgetown University Medical Center, Washington, D.C. 20007.
authors.forename: D
authors.initials: D
authors.lastname: Moody
authors.forename: L J
authors.initials: LJ
authors.lastname: Williams
authors.forename: D M
authors.initials: DM
authors.lastname: Gersten
mesh_terms: D015720:5'-Nucleotidase; D000818:Animals; D002460:Cell Line; D008546:Melanoma, Experimental / Q000201:enzymology*; D051379:Mice; D009708:Nucleotidases / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D009708:Nucleotidases; D015720:5'-Nucleotidase