Modulatory effects of glycosaminoglycans and histamine on lymphocyte mitogenesis.
Since mast cells have previously been shown to be potent modulators of lymphocyte function, the effect of varying concentrations of three mast cell- or basophil- as well as skin ground substance-derived glycosaminoglycans (GAG; heparin, chondroitin sulfate, hyaluronic acid) and of histamine was investigated on rat spleen cell 3H-thymidine incorporation in the absence and presence of mitogens. Modulation proved to be different for the three GAG: chondroitin sulfate was inhibitory and hyaluronic acid enhancing, while heparin exhibited a more complex pattern, with inhibition in control and phytohemagglutinin-stimulated cultures and enhancement in concanavalin A-driven cultures, in parallel to histamine. The data suggest that ground substance GAG as well as mast cell- and basophil-derived mediators in the skin can have a marked and complex modulatory effect on the function of lymphocytes.
Czarnetzki
B M
BM
Department of Dermatology, University Clinics, Münster, FRG.
Wüllenweber
I
I
D000818:Animals; D002809:Chondroitin Sulfates / Q000494:pharmacology; D006025:Glycosaminoglycans / Q000494:pharmacology*; D006493:Heparin / Q000494:pharmacology; D006632:Histamine / Q000494:pharmacology*; D006820:Hyaluronic Acid / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism
D006025:Glycosaminoglycans; D006632:Histamine; D006820:Hyaluronic Acid; D006493:Heparin; D002809:Chondroitin Sulfates; D013936:Thymidine