title: Effect of cyclosporin A on carbohydrate metabolism of isolated rat liver cells.
abstract: Treatment with cyclosporin A may cause hyperglycemia. The mechanisms for this phenomenon are not entirely clear. The effect of cyclosporin A treatment on rat liver phosphoenolpyruvate-carboxykinase and hepatocyte glycogen synthetase and phosphorylase activity was therefore investigated. Glycogen synthetase (total enzyme activity and the active I-form) was elevated in hepatocytes of rats undergoing immunosuppressive treatment with cyclosporin A. Glycogen phosphorylase activity showed no difference compared to hepatocytes from control rats. Activity of phosphoenolpyruvate-carboxykinase in liver homogenates was significantly enhanced after cyclosporin A treatment. Thus, it appears that cyclosporin A treatment stimulates gluconeogenesis, and glucose is used as substrate for glycogen synthesis in vitro. Differences of in vitro and in vivo experiments might be explained by long-term induction of phosphoenolpyruvate-carboxykinase and the short-term effects of cyclosporin A on glycogen synthetase.
authors.affiliation: Department of Internal Medicine, University of Freiburg, Federal Republic of Germany.
authors.forename: W
authors.initials: W
authors.lastname: Riegel
authors.forename: E
authors.initials: E
authors.lastname: Stephan
authors.forename: C
authors.initials: C
authors.lastname: Balle
authors.forename: P
authors.initials: P
authors.lastname: Schollmeyer
authors.forename: A
authors.initials: A
authors.lastname: Heidland
authors.forename: W H
authors.initials: WH
authors.lastname: Hörl
mesh_terms: D000818:Animals; D002469:Cell Separation; D003524:Cyclosporins / Q000494:pharmacology*; D005260:Female; D006006:Glycogen Synthase / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D010730:Phosphoenolpyruvate Carboxylase / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D003524:Cyclosporins; D006006:Glycogen Synthase; D010730:Phosphoenolpyruvate Carboxylase