Nucleotide sequence of the gene encoding the active-site serine beta-lactamase from Actinomadura R39.
The gene encoding the extracellular, active-site serine beta-lactamase of Actinomadura R39, previously cloned into Streptomyces lividans, has the information for the synthesis of a 304 amino acid protein, the amino terminal region of which has the characteristic features of a signal peptide. The Actinomadura R39 beta-lactamase is another member of the class A beta-lactamases. In particular, it shows high homology with the beta-lactamase of Bacillus licheniformis.
Houba
S
S
Service de Microbiologie, Université de Liège, Institut de Chimie, Sart Tilman, Belgium.
Willem
S
S
Duez
C
C
Molitor
C
C
Dusart
J
J
Frère
J M
JM
Ghuysen
J M
JM
D000192:Actinomycetales / Q000201:enzymology / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010957:Plasmids; D015183:Restriction Mapping; D012694:Serine; D013302:Streptomyces / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics*
D004269:DNA, Bacterial; D012694:Serine; D001618:beta-Lactamases