A karyophilic signal sequence in adenovirus type 5 E1A is functional in Xenopus oocytes but not in somatic cells.
The carboxy-terminal five amino acids of the adenovirus type 5 E1A gene product are necessary and sufficient for this protein to become localized in the nuclei of somatic cells. In this report, we demonstrate that E1A contains a second nuclear localization signal, which resides within residues 140 to 185 and which functions in Xenopus oocytes but not Xenopus or mammalian somatic cells. These data demonstrate the cell-type-specific utilization of a nuclear localization signal.
Slavicek
J M
JM
Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545.
Jones
N C
NC
Richter
J D
JD
D017456:Adenovirus Early Proteins; D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000032:analysis*; D005914:Globins / Q000032:analysis; D009856:Oncogene Proteins, Viral / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D009865:Oocytes / Q000032:analysis; D014981:Xenopus
D017456:Adenovirus Early Proteins; D009856:Oncogene Proteins, Viral; D005914:Globins