title: Effect of epithelium on mucus secretion from feline tracheal submucosal glands.
abstract: We studied the effect of airway epithelium on mucus secretion by use of an isolated tracheal submucosal gland preparation reported previously (J. Appl. Physiol. 60: 1237-1247, 1986). Mucus glycoconjugate release from submucosal glands of feline trachea was examined using [3H]glucosamine as a mucus precursor. Isolated glands showed significantly higher secretory responses to cholinergic, alpha-, and beta-adrenergic agonists and dibutyryladenosine 3',5'-cyclic monophosphate (average 400% of control) than the conventional tracheal mucosal explants, which contained epithelium and submucosal tissues in addition to submucosal glands (average 160% of control). The addition of isolated epithelium depressed the secretory response of isolated glands to the same level as that of tracheal explants. However, the supernatant from isolated epithelium failed to inhibit secretory responses to methacholine in isolated glands, suggesting that the epithelium-derived inhibitory factor to secretion may be short-lived. Leukotriene D4 antagonist (FPL 55712), cyclooxygenase and/or lipoxygenase inhibitors (indomethacin or BW 755C) caused no significant change in the inhibitory action of epithelium, suggesting that the inhibition is not due to arachidonic acid metabolites. The newly found secretory inhibitory action of epithelium is of particular interest in the pathogenesis of hypersecretion associated with epithelial damage.
authors.affiliation: First Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sasaki
authors.forename: S
authors.initials: S
authors.lastname: Shimura
authors.forename: H
authors.initials: H
authors.lastname: Sasaki
authors.forename: T
authors.initials: T
authors.lastname: Takishima
mesh_terms: D000818:Animals; D001685:Biological Factors / Q000494:pharmacology; D003994:Bucladesine / Q000494:pharmacology; D002415:Cats; D004848:Epithelium / Q000187:drug effects / Q000502:physiology; D005088:Exocrine Glands / Q000187:drug effects / Q000378:metabolism*; D006001:Glycoconjugates / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D009093:Mucus / Q000187:drug effects / Q000378:metabolism*; D009569:Nitric Oxide; D010277:Parasympathomimetics / Q000494:pharmacology; D013566:Sympathomimetics / Q000494:pharmacology; D014132:Trachea / Q000187:drug effects / Q000378:metabolism*
chemical_list: D001685:Biological Factors; D006001:Glycoconjugates; D006023:Glycoproteins; D010277:Parasympathomimetics; D013566:Sympathomimetics; C047874:gastric mucus glycoproteins; D009569:Nitric Oxide; D003994:Bucladesine