Recruitment to the cytoplasm of a cellular lamin-like protein from the nucleus during a poxvirus infection.
Monoclonal antibodies (Mabs) directed against core proteins of rabbit poxvirus (RPV) have proven effective in the identification of host cell proteins such as RNA polymerase II (Pol II) that may play a role in the infectious process (D. K. Morrison and R. W. Moyer, 1986, Cell 44, 587-596). In this article we describe a Mab that has allowed the detection and characterization of a lamin-like protein derived from the nucleus of the infected cell, which like Pol II is recruited to the cytoplasm following RPV infection. A portion of the gene encoding this protein has been isolated through the screening of a lambda gt11 expression vector library. Sequence analysis of the gene shows it to be derived from a member of the HindIII 1.9-kb repetitive element, a family of mammalian repetitive sequences that are highly conserved. Immunoblot analysis and sequence analysis of the open reading frame show divergent relatedness to certain nuclear lamins. The protein is not, however, one of the three principal lamins characterized to date, but instead appears to be a perinuclear protein related to the highly conserved nuclear lamins that is recruited to the cytoplasm during the infectious process.
Bloom
D C
DC
Department of Microbiology, Vanderbilt University, Nashville, Tennessee 37232.
Massung
R
R
Savage
L
L
Morrison
D K
DK
Moyer
R W
RW
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D002277:Carcinoma; D002460:Cell Line; D002467:Cell Nucleus / Q000032:analysis; D003593:Cytoplasm / Q000032:analysis; D004247:DNA / Q000032:analysis / Q000235:genetics; D005455:Fluorescent Antibody Technique; D006801:Humans; D015151:Immunoblotting; D034882:Lamins; D008175:Lung Neoplasms; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000032:analysis* / Q000235:genetics; D011212:Poxviridae / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D014407:Tumor Cells, Cultured; D014616:Vaccinia virus / Q000235:genetics* / Q000502:physiology
D000911:Antibodies, Monoclonal; D034882:Lamins; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; D004247:DNA