[Effect of acute poisoning of rats with carbaryl on transamination reactions with involving pyruvate. III. Supplementary studies of plasma aminotransferases].
Male Wistar rats were poisoned orally with one carbaryl dose 474 mg/kg (1/2 DL50). After 2, 4, 24 and 72 hours from the administration of the insecticide the activity of plasma aminotransferases catalysing the reactions between pyruvate and the following amino acids: asparagine, aspartic acid, glutamic acid, isoleucine, lysine and valine was determined. Under physiological conditions these amino acids are not participating in the transamination reactions. The activity of aminotransferases was expressed as the amount of alanine developing during incubation of 1 ml of plasma for 1 hour. Alanine was determined spectrophotometrically after chromatographic separation on paper. The results of the investigations demonstrated that acute poisoning with carbaryl caused the appearance of activity of all studied plasma aminotransferases, except for the enzyme catalysing the reaction between pyruvate and lysine. The activity of the studied aminotransferases appeared as follows: glutamic acid--pyruvate--4 hr; isoleucine--pyruvate--24 and 72 hr; asparagine--pyruvate, aspartic acid--pyruvate and valine--pyruvate--72 hr after intoxication.
Jeleniewicz
K
K
D000596:Amino Acids / Q000097:blood; D000818:Animals; D012721:Carbaryl / Q000506:poisoning*; D008297:Male; D011773:Pyruvates / Q000378:metabolism*; D019289:Pyruvic Acid; D051381:Rats; D011919:Rats, Inbred Strains; D000637:Transaminases / Q000097:blood*
D000596:Amino Acids; D011773:Pyruvates; D019289:Pyruvic Acid; D000637:Transaminases; D012721:Carbaryl
Wpływ ostrego zatrucia szczurów karbarylem na reakcje transaminacji z udziałem pirogronianu. Cz. III. Badania uzupełniajace aminotransferaz osoczowych.