Novel fluorogenic substrates containing bimane system for microdetermination of angiotensin I converting enzyme.
As a sensitive fluorometric assay for the activity of angiotensin converting enzyme, bimane-peptides containing tryptophan, i.e., 1,7-dioxo-2,5,6-trimethyl-1H,7H-pyrazolo [1,2-a]pyrazol-3-yl-methylthiomethylcarbonyl-glycyl (or L-phenylalanyl)-L-tryptophyl-L-leucine (or L-proline), were synthesized and shown to be potent fluorogenic substrates for the micro-determination of angiotensin I converting enzyme activity.
Sato
E
E
Nishikawa
S
S
Kanaoka
Y
Y
D001643:Bridged Bicyclo Compounds; D019086:Bridged Bicyclo Compounds, Heterocyclic; D005456:Fluorescent Dyes; D007703:Peptidyl-Dipeptidase A / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D013379:Substrate Specificity
D001643:Bridged Bicyclo Compounds; D019086:Bridged Bicyclo Compounds, Heterocyclic; D005456:Fluorescent Dyes; C021149:bimanes; D007703:Peptidyl-Dipeptidase A