Analysis of amlodipine in serum by a sensitive high-performance liquid chromatographic method with amperometric detection.
An analytical method for a new calcium channel blocking agent, amlodipine, has been developed using high-performance liquid chromatography with electrochemical detection. No compound modification is required for detection and the calibration curve in spiked sera is linear and reproducible over the range 0.2-2.0 ng ml-1. The method has been applied successfully to pharmacokinetics studies in rats and also can be used for other dihydropyridine compounds such as nifedipine and nicardipine.
Shimooka
K
K
Department of Biochemistry, New Product Development Centre, Pfizer Taito Co. Ltd., Japan.
Sawada
Y
Y
Tatematsu
H
H
D017311:Amlodipine; D002121:Calcium Channel Blockers / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008297:Male; D009543:Nifedipine / Q000031:analogs & derivatives* / Q000097:blood
D002121:Calcium Channel Blockers; D017311:Amlodipine; D009543:Nifedipine