Reversal of virus-induced alveolar macrophage bactericidal dysfunction by cyclooxygenase inhibition in vitro.
Virus infection of alveolar macrophages (AM) both in vivo and in vitro has been associated with a decreased ability of these cells to kill bacteria, together with enhanced production of metabolites of arachidonic acid. These metabolites, especially PGE2, may be inhibitory to some phagocyte functions. Primary cultures of bovine AM obtained by bronchoalveolar lavage of normal cattle were infected in vitro with parainfluenza-3 (PI3 virus) virus. Killing of Staphylococcus epidermidis by AM was determined on days 1-4 post-infection (p.i.) PI3 virus-infected AM killed significantly fewer bacteria on day 4 p.i. compared to uninfected controls (12.1 +/- 1.3% infected vs. 52.7 +/- 7.2% controls, P less than or equal to 0.05). Bacterial killing by virus-infected AM, but not control AM, was significantly enhanced on day 4 p.i. by addition of cyclooxygenase inhibitors 1 hr prior to bactericidal assay (28.0 +/- 4.5% indomethacin, 36.0 +/- 4.1% mefenamic acid, 38.6 +/- 7.3% piroxicam, 37.0 +/- 6.4% NDGA, 44.9 +/- 7.7% ETYA, P less than or equal to 0.05). Phagocytosis of opsonized sheep erythrocytes and superoxide generation by virus-infected AM were not significantly increased by cyclooxygenase inhibition. Phagosome-lysosome fusion was severely impaired in virus-infected AM. Pretreatment of virus-infected AM with indomethacin significantly enhanced the percentage of cell expressing fusion activity. This data suggests that in vitro bactericidal dysfunction associated with virus infection of AM is partially the result of enhanced production of prostaglandins or thromboxane by AM and/or an abnormal response to normal levels of endogenously produced cyclooxygenase metabolites. The data further indicate the presence of cyclooxygenase sensitive (phagosome-lysosome fusion) and insensitive (phagocytic) components of virus-induced bactericidal dysfunction in AM.
Laegreid
W W
WW
Department of Veterinary Microbiology, Washington State University, Pullman 99164-7040.
Liggitt
H D
HD
Silflow
R M
RM
Evermann
J R
JR
Taylor
S M
SM
Leid
R W
RW
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D016861:Cyclooxygenase Inhibitors; D008247:Lysosomes / Q000502:physiology; D008264:Macrophages / Q000201:enzymology / Q000276:immunology / Q000382:microbiology*; D008297:Male; D010224:Parainfluenza Virus 3, Human; D018184:Paramyxoviridae Infections / Q000201:enzymology / Q000276:immunology* / Q000382:microbiology; D010587:Phagocytosis; D010588:Phagosomes / Q000502:physiology; D011650:Pulmonary Alveoli; D013212:Staphylococcus epidermidis / Q000254:growth & development / Q000276:immunology*; D013481:Superoxides / Q000096:biosynthesis
D016861:Cyclooxygenase Inhibitors; D013481:Superoxides