title: Distribution in clusters of complement receptor type one (CR1) on human erythrocytes.
abstract: The distribution of CR1 on human E was studied using label-fracture and thin section electron microscopy. CR1 was found to be organized in clusters on unfixed cells and on cells that had been prefixed with paraformaldehyde or glutaraldehyde before labeling. The number of clusters/E ranged from 8 to 20 as estimated from the examination of freeze-fracture replicas of labeled cells. Clusters contained an average of 30 to 75 gold particles on cells from two donors which expressed 462 and 586 CR1 Ag sites/cell, as determined by flow cytometry. In thin section electron micrographs, gold complexes were seen surrounding an electron-dense material protruding from the membrane which represents compact aggregates of CR1. The maximal distance between gold particles and the membrane was 100 nm, which corresponds to the estimated length of the major allotypic form of CR1, as calculated from the primary DNA sequence of the molecule. The distribution in clusters of CR1 on the E membrane may provide the basis for an enhanced affinity of C3b-CR1 interactions on the plasma membrane of the cells and may explain the preferential binding of C3b-bearing immune complexes to E in vivo.
authors.affiliation: INSERM U28, Hôpital Broussais, Paris, France.
authors.forename: J
authors.initials: J
authors.lastname: Chevalier
authors.forename: M D
authors.initials: MD
authors.lastname: Kazatchkine
mesh_terms: D000911:Antibodies, Monoclonal; D003179:Complement C3b / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000032:analysis* / Q000648:ultrastructure; D005404:Fixatives; D005614:Freeze Fracturing; D006046:Gold; D006801:Humans; D007150:Immunohistochemistry; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D011951:Receptors, Complement / Q000032:analysis* / Q000276:immunology; D017463:Receptors, Complement 3b
chemical_list: D000911:Antibodies, Monoclonal; D005404:Fixatives; D046911:Macromolecular Substances; D011951:Receptors, Complement; D017463:Receptors, Complement 3b; D006046:Gold; D003179:Complement C3b