[The analysis of gene-regulatory mechanism of ganglioside metabolism by gene transfection].
To investigate the genetic control on ganglioside metabolism, various oncogenes were directly transfected to rat 3Y1 cells, and the subsequent alteration in the ganglioside pattern was analysed. So-called "intranuclear" type oncogenes, the products of which are mainly expressed intranuclearly, ex. adeno E1 and myc, brought about specifically GD3 ganglioside with concomitant decrease in GM3. On the other hand, so-called "extranuclear" type oncogenes, the products of which are expressed extranuclearly (either on the cell membrane or in the cytoplasm), ex. ras and src, brought about SPG gangliosides but no GD3 expression was recognized. This result indicates that intracellular metabolic activities of oncogenes strongly affect the ganglioside metabolism, and that the sensitive points in the pathway of the ganglioside metabolism (synthesis) are not so various but rather restricted.
Nakaishi
H
H
Second Department of Biochemistry, Faculty of Medicine, University of Tokyo, Japan.
D000818:Animals; D002478:Cells, Cultured; D005732:Gangliosides / Q000378:metabolism*; D005915:Globosides / Q000378:metabolism; D009857:Oncogenes; D051381:Rats; D014162:Transfection
D005732:Gangliosides; D005915:Globosides; C026226:ganglioside, GD3; C014812:sialosylparagloboside