[Outer membranes of Francisella tularensis and their protein composition].
Four strains of the species Francisella tularensis were used in the present work: a live vaccine strain 15/10 and three virulent strains (503, Schu, 543) from three different subspecies. The bacterial membranes were prepared by the 0.5% N-laurylsarcosinate (Sarcosyl) treatment. These membranes were identified as the outer membranes by morphological, immunological and biochemical analyses. The outer membrane proteins contained up to 30-35 polypeptides with three dominant fractions having the 63, 48 and 41-43 kD molecular masses. Despite the significant similarity between the membranes protein profiles there were some quantitative and qualitative differences between the three variants of Francisella tularensis in polypeptides compositions and patterns.
Rodionov
I V
IV
Konstantinova
N D
ND
Brukhanskiĭ
G V
GV
D001425:Bacterial Outer Membrane Proteins / Q000032:analysis* / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D005604:Francisella tularensis / Q000032:analysis* / Q000472:pathogenicity / Q000648:ultrastructure; D005779:Immunodiffusion; D008970:Molecular Weight; D014774:Virulence
D001425:Bacterial Outer Membrane Proteins
Naruzhnye membrany tuliaremiĭnogo mikroba i ikh belkovyĭ sostav.