Elucidation of a role of plasma albumin during collagen-induced aggregation of bovine platelets.
Bovine platelets of which phospholipids were labeled with [14C] arachidonate were stimulated by collagen. Omitting albumin in suspending medium suppressed aggregation and arachidonate liberation from phospholipids. Analysis of [14C] arachidonate metabolites released into medium revealed that release of 12S-hydroxyeicosatetraenoic acid (12-HETE) from stimulated platelets was dependent on the existence of albumin, while thromboxane B2 release was not. Moreover, exogenously added 12-HETE inhibited collagen-induced aggregation. These observations suggest that albumin potentiates aggregation by preventing intracellular accumulation of 12-HETE, and strengthen our previous finding that albumin in plasma is very essential for collagen-induced aggregation of platelets (Sekiya, F., Takagi, J., Kasahara, K., Inada, Y. and Saito, Y. (1988) Thrombos. Res. 50, 837-846.).
Sekiya
F
F
Department of Biological Sciences, Tokyo Institute of Technology, Japan.
Takagi
J
J
Saito
Y
Y
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D001792:Blood Platelets / Q000378:metabolism; D002417:Cattle / Q000097:blood; D003094:Collagen / Q000494:pharmacology*; D006893:Hydroxyeicosatetraenoic Acids / Q000378:metabolism / Q000494:pharmacology; D010743:Phospholipids / Q000378:metabolism; D010974:Platelet Aggregation / Q000187:drug effects* / Q000502:physiology; D010975:Platelet Aggregation Inhibitors; D012710:Serum Albumin, Bovine / Q000502:physiology*; D013268:Stimulation, Chemical; D013931:Thromboxanes / Q000096:biosynthesis
D001095:Arachidonic Acids; D006893:Hydroxyeicosatetraenoic Acids; D010743:Phospholipids; D010975:Platelet Aggregation Inhibitors; D013931:Thromboxanes; D012710:Serum Albumin, Bovine; D016718:Arachidonic Acid; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D003094:Collagen