Rearranging sequence located in the intron of the human T cell receptor gamma chain gene constant region.
Human T cell rearranging gamma genes encode membrane proteins which are expressed by a minor subset of T cells. Extensive studies have shown that TcR gamma gene rearrangements result from V gamma-J gamma recombinations. Here we analyze an unusual rearrangement involving a non-translatable sequence flanked by heptamer and nonamer signals in a Ti alpha/beta-positive T cell clone (JF1). This sequence, designated here as I gamma RS, is distinct from V, D or J elements. It is located in the first intron of the first constant region (C gamma 1) and homologous sequences are conserved in C gamma 2 introns. In JF1 cells the I gamma RS is juxtaposed to the J gamma 2 segment downstream to a V gamma 10-J gamma 1 rearrangement. The use of a cryptic splicing site induced JF1 cells to produce an aberrant large-sized transcript containing the I gamma RS 3' to the first exon of C gamma 1. Such a rearrangement occurring downstream to an inframe V gamma-J gamma junction may induce premature arrest of translation and lack of the C-terminal part of C gamma involved in gamma-protein assembly.
Chen
Z
Z
INSERM U 301, Hôpital Saint Louis, Paris, France.
Loiseau
P
P
Bories
J C
JC
Bensussan
A
A
Sigaux
F
F
D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta; D015183:Restriction Mapping; D013601:T-Lymphocytes / Q000502:physiology*
D012333:RNA, Messenger; D011948:Receptors, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta