The distribution of glycosyl-phosphatidylinositol anchored proteins is differentially regulated by serum and insulin.
Glycosyl-phosphatidylinositol (GPI) anchored proteins are surveyed in two insulin sensitive cell types by surface labeling and phospholipase C-induced release into the medium. Serum starvation selectively increases both the number and intensity of a subset of GPI-anchored proteins. After serum starvation, loss of cell-surface GPI-anchored proteins is induced acutely by either serum re-exposure or insulin, suggesting that hormonal treatment may promote the release of these proteins from the cell surface.
Lisanti
M P
MP
Department of Cell Biology and Anatomy, Cornell University Medical College, New York, NY 10021.
Darnell
J C
JC
Chan
B L
BL
Rodriguez-Boulan
E
E
Saltiel
A R
AR
D000818:Animals; D001769:Blood; D002478:Cells, Cultured; D003470:Culture Media; D006017:Glycolipids / Q000378:metabolism*; D017261:Glycosylphosphatidylinositols; D007328:Insulin / Q000494:pharmacology; D051379:Mice; D008970:Molecular Weight; D009132:Muscles / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism*; D010738:Type C Phospholipases
D003470:Culture Media; D006017:Glycolipids; D017261:Glycosylphosphatidylinositols; D007328:Insulin; D010716:Phosphatidylinositols; D010738:Type C Phospholipases