Interactions between Sarcocystis gigantea lectin and toxin-containing fractions in human lymphocyte cultures.
Sarcocystis gigantea extract (SGE) was separated by affinity chromatography into one lectin-containing fraction (SGL) that was mitogenic to mononuclear cells (MNC) and another that lacked this lectin activity. The SGL-depleted Sarcocystis extract (SGTF) contained the so-called Sarcotoxin, inducing only a slight increase in MNC proliferation. Furthermore, preincubation of MNC with SGTF for 60 min suppressed the mitogenic capacity of SGL by 60%-90%. The results presented indicate that SGTF interacts with human MNC differently than SGL, particularly by interfering with the mitogenic lectin. These findings suggest that SGL and SGTF may be involved in different immunomechanisms induced by the parasite.
Tietz
H J
HJ
Institut für Medizinische Mikrobiologie des Bereichs Medizin (Charité), Berlin.
Montag
T
T
Brose
E
E
Widera
P
P
Sokolowska-Köhler
W
W
Mann
W
W
Hiepe
T
T
D000818:Animals; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D006801:Humans; D037102:Lectins / Q000302:isolation & purification / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology; D012522:Sarcocystis / Q000032:analysis / Q000276:immunology*; D014118:Toxins, Biological / Q000302:isolation & purification / Q000494:pharmacology*
D037102:Lectins; D014118:Toxins, Biological