[Comparative histology and immunohistochemistry of tests of immediate allergic reaction and type I contact dermatitis].
Contrary to type IV contact dermatitis, histochemical studies of immunological mechanisms in type I contact dermatitis are rare. To analyse the immunohistochemical kinetics of immediate type I reactions we followed up prick-test reactions to individual specific allergens in 5 atopics with respiratory allergy (group I) and in 4 patients with proven type I contact dermatitis (group II). Punch biopsies (diameter 6 mm) were taken 20 minutes, 6, 24 and 72 h after prick testing. Histological investigations were performed on paraffin-embedded tissue; immunohistochemical studies were done one frozen tissue with a panel of monoclonal antibodies applying the alkaline-phosphatase-anti-alkaline-phosphatase-complex method. In type I contact dermatitis a moderate lympho-monocytic dermal infiltrate appeared, which increased until 72 hrs after testing. In contrast, the immediate allergic reactions of group I revealed only mild lympho-monocytic infiltration in all proven sequential biopsies. No quantitative differences concerning the immunohistochemical pattern of OKM-1, OKM-5, Leu-2a and Leu-3a were observed between the two groups. Group II showed an increase in HLA DR, OKT6, anti-IgE- and anti-IL-2-receptor immunoreactivity whereas group I exhibited only moderate or no immunoreactivity. A dendritic anti-IgE-staining pattern, most probably on Langerhans cells, was only observed in the epidermis of group II patients. A pathogenetic concept of IgE-mediated type I contact dermatitis is presented and possible relationships with atopic dermatitis are discussed.
Gruschwitz
M
M
Klinik für Dermatologie, Universität Erlangen-Nürnberg.
Wolf
H
H
Hornstein
O P
OP
D000293:Adolescent; D000328:Adult; D000944:Antigens, Differentiation, B-Lymphocyte / Q000378:metabolism; D003877:Dermatitis, Contact / Q000175:diagnosis* / Q000209:etiology / Q000473:pathology; D005260:Female; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000175:diagnosis* / Q000473:pathology; D007073:Immunoglobulin E / Q000032:analysis; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D011961:Receptors, Fc / Q000378:metabolism; D017455:Receptors, IgE; D015375:Receptors, Interleukin-2 / Q000378:metabolism; D012882:Skin Tests
D000944:Antigens, Differentiation, B-Lymphocyte; D011961:Receptors, Fc; D017455:Receptors, IgE; D015375:Receptors, Interleukin-2; D007073:Immunoglobulin E
Etude histologique et immunohistochimique comparative des tests d'une réaction allergique immédiate et d'une dermite de contact de type I.