A longitudinal study on the expression of the opsin gene in the degenerating retina of C3H/He mice.
By a combination of immunohistochemistry and in situ hybridization using a 35S-labeled opsin cDNA probe, it was revealed in rd mutant C3H/He mice that opsin mRNA is not detectable in photoreceptor cells which still exhibit opsin-immunoreactivity at later stages of retinal degeneration. This indicates that the photoreceptor cells of rd mutant mice cease to express the opsin gene at earlier stages of retinal degeneration.
Iseki
S
S
Department of Anatomy, Kanazawa University School of Medicine, Japan.
Kondo
H
H
Kuo
C H
CH
Miki
N
N
D000818:Animals; D004247:DNA / Q000235:genetics; D005136:Eye Proteins / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics; D012162:Retinal Degeneration / Q000378:metabolism* / Q000473:pathology; D012168:Retinal Pigments / Q000235:genetics / Q000378:metabolism; D017299:Rod Opsins
D005136:Eye Proteins; D012333:RNA, Messenger; D012168:Retinal Pigments; D017299:Rod Opsins; D004247:DNA