Monocyte Fc gamma receptor recognition of cell-bound and aggregated IgG.
Monocyte and macrophage Fc gamma receptors are important components in the recognition of IgG-coated cells and IgG-containing immune complexes. Two proteins have been identified on human peripheral blood monocytes that can function as Fc gamma receptors, Fc gamma RI (70 Kd) and Fc gamma RII (40 Kd). We studied the role of Fc gamma RI and Fc gamma RII on human monocytes by examining their binding of IgG-sensitized cells (human IgG anti-D-coated RBCs and rabbit IgG-sensitized sheep RBCs) and their binding of human trimeric IgG. To examine the function of monocyte Fc gamma RII, we used an anti-Fc gamma RII monoclonal antibody (MoAb) that competes for the Fc gamma RII ligand binding site. Preincubation of monocytes with saturating concentrations of anti-Fc gamma RII MoAb did not alter the recognition of IgG (anti-D)-sensitized human RBCs by monocytes. Furthermore, ligand-binding studies demonstrated that anti-Fc gamma RII antibody altered neither the number nor the affinity of monocyte-binding sites for human IgG trimer. Anti-Fc gamma RII inhibited monocyte binding of rabbit IgG-sensitized sheep RBCs, but only at low ionic strength or temperature when increased numbers of monocyte Fc gamma RII were expressed. At low ionic strength and 4 degrees C, anti-Fc gamma RII also partially inhibited monocyte binding of human trimeric IgG. Thus, monocyte Fc gamma RII does not appear to recognize IgG-sensitized RBCs or trimeric IgG at physiologic temperatures and ionic strength. The data suggest that Fc gamma RI is the primary Fc gamma receptor on monocytes involved in the binding of IgG (anti-D)-sensitized erythrocytes and low mol wt complexes of IgG.
Gomez
F
F
University of Pennsylvania Cancer Center, Philadelphia 19104.
Chien
P
P
King
M
M
McDermott
P
P
Levinson
A I
AI
Rossman
M D
MD
Schreiber
A D
AD
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology; D000943:Antigens, Differentiation / Q000276:immunology / Q000502:physiology*; D004912:Erythrocytes / Q000276:immunology; D006801:Humans; D007114:Immunization; D007074:Immunoglobulin G / Q000378:metabolism*; D007518:Isoantibodies; D046911:Macromolecular Substances; D009000:Monocytes / Q000378:metabolism*; D011817:Rabbits; D011961:Receptors, Fc / Q000276:immunology / Q000502:physiology*; D017452:Receptors, IgG; D012204:Rh-Hr Blood-Group System / Q000276:immunology; D012756:Sheep
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D007074:Immunoglobulin G; D007518:Isoantibodies; D046911:Macromolecular Substances; C033242:RH-antibodies; D011961:Receptors, Fc; D017452:Receptors, IgG; D012204:Rh-Hr Blood-Group System