Is lysosomal fusion required for the granulocyte chemiluminescence reaction?
When phagocytic leukocytes interact with soluble or particulate stimuli, the cells increase their production of oxidative metabolites. This increased production can be measured as luminol amplified light emission or chemiluminescence. From the literature it can be concluded that the chemiluminescence reaction is dependent on oxygen radicals produced by the cells and on the enzyme myeloperoxidase. Since the radical producing system and the peroxidase are localized to different subcellular compartments, it is proposed that a lysosomal fusion, bringing the two reactants together into the same subcellular compartment, is a prerequisite for the chemiluminescence reaction.
Dahlgren
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Department of Medical Microbiology, University of Linköping, Sweden.
D000818:Animals; D006098:Granulocytes / Q000502:physiology*; D006801:Humans; D008163:Luminescent Measurements; D008247:Lysosomes / Q000502:physiology*; D008962:Models, Theoretical; D009195:Peroxidase / Q000097:blood; D010587:Phagocytosis
D009195:Peroxidase