title: Experimental allergic encephalomyelitis in the monkey: humoral immunity and blood-brain barrier function.
abstract: Acute monophasic experimental autoimmune encephalomyelitis (AMEAE) was induced in 5 Macacus cynomolgus monkeys. Serum and cerebrospinal fluid (CSF) paired samples were collected before and four weeks after immunization, time of complete development of AMEAE clinical picture. After immunization the CSF/serum albumin ratio and the IgG index were markedly increased. Agarose isoelectric focusing (AIEF) followed by immunofixation revealed faint IgG oligoclonal bands in both serum and CSF of all monkeys, which became more evident after Kappa and Lambda assessment. Intrathecal synthesis of IgG oligoclonal bands was detected in only one CSF. Affinity-driven immunoblotting failed to detect anti-myelin basic protein (MBP) oligoclonal IgG. No free light chain (FLC) patterns or IgA and IgM oligoclonal bands were detected.
authors.affiliation: Clinica Neurologica, Università di Padova.
authors.forename: P
authors.initials: P
authors.lastname: Gallo
authors.forename: D
authors.initials: D
authors.lastname: Cupic
authors.forename: F
authors.initials: F
authors.lastname: Bracco
authors.forename: L
authors.initials: L
authors.lastname: Krzalic
authors.forename: B
authors.initials: B
authors.lastname: Tavolato
authors.forename: L
authors.initials: L
authors.lastname: Battistin
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology*; D005260:Female; D007143:Immunoglobulin Heavy Chains / Q000378:metabolism*; D007142:Immunoglobulin gamma-Chains / Q000134:cerebrospinal fluid / Q000378:metabolism*; D008251:Macaca / Q000276:immunology*; D008252:Macaca fascicularis / Q000276:immunology*; D008297:Male
chemical_list: D007143:Immunoglobulin Heavy Chains; D007142:Immunoglobulin gamma-Chains