Detection of antibodies to caprine arthritis-encephalitis virus by protein G enzyme-linked immunosorbent assay and immunoblotting.
Sera from goats suffering from caprine arthritis-encephalitis contained antibodies to virus proteins of 15, 17, 28, 40, and 130 kilodaltons in immunoblots of maedi-visna virus. We propose to use immunoblotting as a validation test for enzyme-linked immunosorbent assay and demonstrate that the specificity of indirect enzyme-linked immunosorbent assay can be improved by replacing second antibody by a protein G-avidin-biotin conjugate.
Zanoni
R
R
Institute of Veterinary Virology, University of Bern, Switzerland.
Krieg
A
A
Peterhans
E
E
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D001170:Arthritis, Infectious / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary; D003429:Cross Reactions; D004660:Encephalitis / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay; D006041:Goats; D015151:Immunoblotting; D009419:Nerve Tissue Proteins; D011237:Predictive Value of Tests; D012190:Retroviridae / Q000276:immunology*; D012192:Retroviridae Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D013379:Substrate Specificity
D000914:Antibodies, Viral; C029152:G-substrate; D009419:Nerve Tissue Proteins