[Flow cytometric analysis of surface phenotypes in B-cell non-Hodgkin's lymphoma].
The objective of this study is to demonstrate the diagnostic usefulness of flow cytometric analysis of surface immunoglobulin (S-Ig) light chains and monoclonal antibodies (MoAbs) in B-cell non-Hodgkin's lymphoma. For this purpose, the biopsied specimen (lymph nodes, tonsils, and spleens etc) cell suspensions from 44 patients were studied to detect the expression of S-Ig and several antigens recognized by MoAbs. A tumor was considered B-lineage if expression of pan B antigens and/or monoclonal light chains (S-Ig and/or cytoplasmic Ig) was detected in the absence of pan T antigens. Monoclonal expression of S-Ig light chain was detectable in 37 of 44 (84%) patients with B-cell lymphoma. In two of three cases having polyclonal S-Ig light chain, monoclonal C-Ig light chain was detected in large cells on paraffin-embedded tissues with immunohistochemical technique. Pan B antigens were strongly positive in four cases with negative S-Ig light chain. In diffuse large cell lymphoma, expression of CD11b was restricted to non-cleaved cell type (DLN). CD10 was favorably expressed by non-cleaved cell type and to a lesser extent, by immunoblastic histology (LI), but was not detected on cases with cleaved cell type (DLC). Furthermore, relatively uniform expression of S-IgM was seen in DLC subgroup, and on the contrary, heterogenous S-Ig was shown in other histology groups (DLN, LI). Flow cytometry provides a rapid, objective technology to confirm the immunological diagnosis of B-cell non-Hodgkin's lymphoma.
Chubachi
A
A
D000911:Antibodies, Monoclonal; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000032:analysis; D008228:Lymphoma, Non-Hodgkin / Q000175:diagnosis* / Q000276:immunology; D010641:Phenotype; D011947:Receptors, Antigen, B-Cell / Q000032:analysis*
D000911:Antibodies, Monoclonal; D000944:Antigens, Differentiation, B-Lymphocyte; D007147:Immunoglobulin Light Chains; D011947:Receptors, Antigen, B-Cell