Immunization with syngeneic Sendai virus-infected cells induce no MHC-restricted antibodies but antibodies specific for H-2 class I determinants.
To find out whether immunoglobulins are able to recognize foreign antigens in the context of syngeneic MHC determinants, an effort was made to trigger the production of MHC-restricted antibodies by syngeneic Sendai virus (SV)-infected cells using the spleen-fragment culture technique. Antibodies were found that mimicked MHC-restricted antibodies by recognizing MHC+SV better than MHC alone. However, the binding was not specific for SV and also occurred on mitogen-stimulated (SV-) or influenza virus-infected cells. We describe the production of H-2 class I-specific lymphocytotoxic antibodies by primary B cells responding to syngeneic SV-infected cells. No viral-specific, H-2-restricted antibodies were found.
Kievits
F
F
Central Laboratory, Netherlands Red Cross Blood Transfusion Service, Amsterdam.
Boerenkamp
W J
WJ
Ivanyi
P
P
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D001402:B-Lymphocytes / Q000276:immunology*; D002472:Cell Transformation, Viral; D003602:Cytotoxicity, Immunologic; D006183:H-2 Antigens / Q000276:immunology*; D007518:Isoantibodies / Q000276:immunology*; D008285:Major Histocompatibility Complex; D051379:Mice; D010222:Parainfluenza Virus 1, Human; D013154:Spleen / Q000276:immunology
D006183:H-2 Antigens; D007518:Isoantibodies