[Study on rapid serodiagnosis for leprosy].
In this article, we have developed two latex agglutination reaction tests (LAR) with PGL-1 and ND-O-BSA as antigens in 80 cases of leprosy patients (LL = 20, BL = 20, BT = 20, TT = 20), 20 cases of tuberculosis and 25 cases of normal control. These two latex agglutination tests were compared with relevant ELISAs (ND-O-BSA- and PGL-1-ELISA), and analysed by Chi-square (chi 2) test. The results indicated that the sensitivity and specificity of the two LARs (PGL-1- and ND-O-BSA-LAR) are similar to their relevant ELISAs respectively (PGL-1-LAR Vs ND-O-BSA-LAR, chi 2 = 1.785, P greater than 0.10; PGL-1-LAR Vs PGL-1-ELISA, chi 2 = 2.0, P greater than 0.10; ND-O-BSA-LAR Vs ND-O-BSA-ELISA, chi 2 = 0.972, P greater than 0.25), and the increase of positive rate is coincident with clinical classification of leprosy. Since LARs are more simple and rapid than ELISAs, LARs may become more useful tools for immunodiagnosis of leprosy in the field. Also conditions to ensure the stability and reproducibility of LARs are discussed in detail.
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D000942:Antigens, Bacterial / Q000032:analysis; D004187:Disaccharides; D004797:Enzyme-Linked Immunosorbent Assay; D006017:Glycolipids / Q000032:analysis; D006801:Humans; D007841:Latex Fixation Tests / Q000379:methods*; D007918:Leprosy / Q000175:diagnosis*; D009166:Mycobacterium leprae / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000032:analysis
D000942:Antigens, Bacterial; D004187:Disaccharides; D006017:Glycolipids; C051559:O-(3,6-di-O-methylglucopyranosyl)-(1-4)-O-(2,3-di-O-methylrhamnopyranosyl)-(1-9)-oxynonanoyl-bovine serum albumin; C045169:phenolic glycolipid I, Mycobacterium leprae; D012710:Serum Albumin, Bovine