Human Fc epsilon R II and IgE-binding factors.
The low-affinity receptor for IgE (Fc epsilon R II) is mainly expressed on B lymphocytes, although it may also be found on some monocytes, eosinophils, and platelets. The presence of Fc epsilon R II on T cells is still controversial, but our results demonstrate that it is expressed on some HTLV-I-transformed T lymphocytes, and they strongly suggest that it may be found on a small proportion of normal T cells. Fc epsilon R II is a 45-KD glycoprotein containing one N-linked carbohydrate of complex type, O-linked carbohydrates, and sialic acid residues. Fc II is cleaved into soluble fragments with molecular weights of 37, 33, 25, and 12 KD, the first three retain the ability of binding to IgE, i.e., they are IgE-binding factors (IgE-BFs). The enzymes involved in their proteolytic cleavage are cell bound. The cDNA coding for Fc epsilon R II was cloned and functionally expressed. The predicted sequence has no homology with that of murine IgE-BFs which are of T cell origin. However, there is a striking homology with several animal lectins, and since the IgE-binding site is located in the homology region, it is possible that it binds to IgE via the carbohydrates expressed on the Fc region of this immunoglobulin. The expression of Fc epsilon R II on B cells and the release of IgE-BFs are upregulated by interleukin 4 and suppressed by gamma and alpha interferons.(ABSTRACT TRUNCATED AT 250 WORDS)
Delespesse
G
G
University of Montreal, Notre-Dame Hospital, Canada.
Sarfati
M
M
Hofstetter
H
H
Frost
H
H
Kilchherr
E
E
Suter
U
U
D000944:Antigens, Differentiation, B-Lymphocyte / Q000502:physiology*; D001773:Blood Cells / Q000502:physiology; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis / Q000378:metabolism*; D015847:Interleukin-4; D007378:Interleukins / Q000502:physiology; D008222:Lymphokines / Q000502:physiology*; D029584:Prostatic Secretory Proteins; D011961:Receptors, Fc / Q000502:physiology*; D017455:Receptors, IgE
D000944:Antigens, Differentiation, B-Lymphocyte; D007378:Interleukins; D008222:Lymphokines; D029584:Prostatic Secretory Proteins; D011961:Receptors, Fc; D017455:Receptors, IgE; C043076:beta-microseminoprotein; C013059:immunoglobulin-binding factors; D015847:Interleukin-4; D007073:Immunoglobulin E