Studies on the antigenic S-type lipopolysaccharides of Brucella abortus strains 7 and Mustapha.
Antigenic phenol phase S-type lipopolysaccharides (LPS) isolated from Brucella abortus (B. abortus) strains 7 and Mustapha were observed to have 13C n.m.r. spectra which were almost identical to the one reported for the Brucella abortus 1119-3. The glycosyl content of the lipid A obtained from the LPS of strain 7 was found to be 2-acetamido-2-deoxyglucose only while strain Mustapha was found to contain both 2-acetamido-2-deoxyglucose and 2-acetamido-2-deoxygalactose. The fatty acid present in the lipid A of both strains was mainly n-hexadecanoic acid. Octadecanoic acid, 3-hydroxytetradecanoic acid as well as small quantities of 3-hydroxydodecanoic acid were also identified. This contrasts with the earlier reports of the absence of 3-OH-14:0 in the LPS of Brucella abortus.
Adeyeye
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Chemistry Department, University of Lagos, Yaba, Nigeria.
Ogunlesi
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Eze
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D002003:Brucella abortus / Q000276:immunology*; D002849:Chromatography, Gas; D005227:Fatty Acids / Q000032:analysis; D008050:Lipid A / Q000032:analysis; D008070:Lipopolysaccharides / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D010169:Palmitic Acids / Q000032:analysis
D005227:Fatty Acids; D008050:Lipid A; D008070:Lipopolysaccharides; D010169:Palmitic Acids