title: Isolation and characterization of cadmium-resistant mutants of Neurospora crassa.
abstract: This study identified and characterized four cadmium-resistant mutants of Neurospora crassa. One of these mutants maps to linkage group II and the other three map to linkage group VII, whereas a naturally occurring resistant trait in a strain from Japan resides at a distinct but unmapped locus. Transport of cadmium into Neurospora cells occurs by more than a single uptake system and involves both energy-dependent and -independent components. The resistant mutants transport cadmium in the same manner as does the cadmium-sensitive wild-type strain. Cadmium resistance in these mutants does not appear to result from an increase in cytosolic heat-stable cadmium-binding proteins. Cadmium does not induce the typical heat-shock response in conidia. Under various growth conditions, each of the mutants exhibited morphological alterations, possibly involving the cell wall or plasma membrane.
authors.affiliation: Department of Biochemistry, Ohio State University, Columbus 43210.
authors.forename: W B
authors.initials: WB
authors.lastname: Levine
authors.forename: G A
authors.initials: GA
authors.lastname: Marzluf
mesh_terms: D001693:Biological Transport, Active; D002104:Cadmium / Q000378:metabolism / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D005656:Fungal Proteins / Q000096:biosynthesis; D006360:Heat-Shock Proteins / Q000096:biosynthesis; D009154:Mutation; D009491:Neurospora / Q000187:drug effects*; D009492:Neurospora crassa / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D006360:Heat-Shock Proteins; D002104:Cadmium