Cross-reactions of nucleic acids with monoclonal antibodies to phosphatidylinositol phosphate and cholesterol.
Four monoclonal IgM antibodies to phosphatidylinositol phosphate (PIP), four antibodies to cholesterol and one antibody to liposomes containing phosphatidylcholine, cholesterol and dicetyl phosphate were tested for reactivity with denatured DNA. Three of four antibodies to PIP cross-reacted strongly with denatured DNA. The other antibodies did react with denatured DNA but only very weakly. The binding to DNA was competed by synthetic polynucleotides. In competitive assays, one of the anti-PIP antibodies was particularly reactive with poly(dT) and another with poly(I) and poly(dG). Binding of an anti-cholesterol antibody to ssDNA was also inhibited by poly(I) and poly(dG). Two of the anti-PIP antibodies were also reactive with mononucleotides, and all four bound inositol hexaphosphate. High concns of nucleosides did not compete for binding, indicating that phosphate is involved in the binding site. Phospholipids, particularly those containing inositol phosphate, also competed for binding to DNA, but to varying extents, indicating a variable overlap in the antibody binding site for DNA and phospholipid determinants. These antibodies, induced by immunization with liposomes, showed cross-reactivity characteristics often found with certain types of autoantibodies, but they did not bear the H130 idiotype, which was identified on IgM anti-DNA autoantibodies from MRL-lpr/lpr mice.
Stollar
B D
BD
Department of Biochemistry, Tufts University Health Science Campus, Boston, MA 02111.
McInerney
T
T
Gavron
T
T
Wassef
N M
NM
Swartz
G M
GM
Alving
C R
CR
D000818:Animals; D001667:Binding, Competitive; D002784:Cholesterol / Q000276:immunology*; D003429:Cross Reactions; D004277:DNA, Single-Stranded / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D010716:Phosphatidylinositols / Q000276:immunology*; D011068:Poly G / Q000276:immunology; D011069:Poly I / Q000276:immunology; D011071:Poly T / Q000276:immunology
D004277:DNA, Single-Stranded; D007075:Immunoglobulin M; D010716:Phosphatidylinositols; D011071:Poly T; D011068:Poly G; D011069:Poly I; C026628:poly(dG); D002784:Cholesterol