title: Collaboration of Th1 and Th2 T cell clones in specific antibody responses: regulation of the IgM response to phosphorylcholine.
abstract: Carrier (KLH)-specific type 1 T cell clones (Th1), which are defined by secretion of IL-2 and IFN-gamma but not IL-4, and type 2 (Th2) clones, which secrete IL-4, but not IL-2 or IFN-gamma, have been isolated and analyzed for their ability to collaborate in providing help for B cells to secrete phosphorylcholine-specific IgM antibodies. The resulting antibody responses exhibited a characteristic pattern suggesting two distinct regulatory interactions among the Th1, Th2, and B cells. At low doses of antigen, Th1 cells enhanced the helper function of the Th2 cells, an effect due primarily to IL-2. At high doses of antigen, Th1 cells or IFN-gamma inhibited Th2-dependent antibody responses. The inhibitory effect of Th1 or IFN-gamma affected primarily the hapten-carrier-linked portion of the response. The overall effect was a modulation of the antigen dose-response curve for antibody production, eliminating the sharp increases in dose response mediated by isolated T cell clones. The data suggest that collaborative interactions of Th1 and Th2 cells in antibody production may have important physiological consequences.
authors.affiliation: Department of Pediatrics, University of Texas Health Science Center, San Antonio 78284-7805.
authors.forename: A J
authors.initials: AJ
authors.lastname: Infante
authors.forename: P F
authors.initials: PF
authors.lastname: Currier
mesh_terms: D000818:Animals; D002794:Choline / Q000031:analogs & derivatives*; D002999:Clone Cells; D004306:Dose-Response Relationship, Immunologic; D006433:Hemocyanins / Q000276:immunology; D007075:Immunoglobulin M / Q000096:biosynthesis*; D007371:Interferon-gamma / Q000494:pharmacology; D007376:Interleukin-2 / Q000502:physiology; D015847:Interleukin-4; D007378:Interleukins / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D010767:Phosphorylcholine / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
chemical_list: D007075:Immunoglobulin M; D007376:Interleukin-2; D007378:Interleukins; D010767:Phosphorylcholine; D015847:Interleukin-4; D007371:Interferon-gamma; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin; D002794:Choline