Detection of specific binding of [1,6-3H]2,3,7,8-tetrachlordibenzo-p- dioxin ([3H]TCDD) in human leukocytes using electrofocusing in polyacrylamide gel.
The binding of [1,6-3H]2,3,7,8-tetrachlorodibenzo-p-dioxin ([3H]TCDD) in human leukocyte cytosol has been studied using electrofocusing in polyacrylamide gel. One single peak of bound [3H]TCDD is found after completed focusing with an isoelectric point of 6.0. The binding capacity for 1.5 nM [3H]TCDD in the leukocyte cytosol was completely saturated by incubation in the presence of 150 nM unlabelled 2,3,7,8-tetrachlorodibenzofuran. [3H]TCDD also binds to a single binding species in human serum and plasma which focuses at pH 5.2 and which is not saturated by the addition of a 100-fold excess of unlabelled 2,3,7,8-tetrachlorodibenzofuran to the incubation. The levels of specific binding of [3H]TCDD in leukocytes from healthy blood donors ranged from 0 to 50 fmol/mg cytosolic protein.
Gillner
M
M
Department of Medical Nutrition, Karolinska Institute, Huddinge University Hospital, Sweden.
Haldosén
L A
LA
Gustafsson
S A
SA
Gustafsson
J A
JA
D001665:Binding Sites; D003600:Cytosol / Q000032:analysis; D004147:Dioxins / Q000097:blood*; D006801:Humans; D007525:Isoelectric Focusing; D007938:Leukemia / Q000097:blood; D007962:Leukocytes / Q000032:analysis*; D000072317:Polychlorinated Dibenzodioxins / Q000097:blood*; D018336:Receptors, Aryl Hydrocarbon; D011955:Receptors, Drug / Q000032:analysis*; D012588:Scintillation Counting
D004147:Dioxins; D000072317:Polychlorinated Dibenzodioxins; D018336:Receptors, Aryl Hydrocarbon; D011955:Receptors, Drug