[The red light of the helium-neon laser reactivates superoxide dismutase].
The effect of low-energy helium-neon laser (HNL) on enzymatic activity, absorbtion spectra and electron paramagnetic resonance (EPR) signals of superoxide dismutase (SOD) from bovine erythrocytes in acid medium were investigated. It was found that incubation during 2 hours at pH 5.9 led to eventually complete inactivation of the enzyme. The subsequent illumination of inactivated SOD by HNL brought about the enzyme reactivation. Both absorption and EPR-spectra were changed after incubation at pH 5.9. These changes may be attributed to protonation of histidine residue in the enzyme active site. After laser irradiation both absorption and EPR spectra were restored to those typical of native enzyme at pH 8.2. In a model system, copper-histidine complex, absorption maximum was shifted from 632-633 nm at pH 5.8 to 639-640 nm at pH 8.5-9.0. The similar long-wave length shift of the maximum was observed after illumination by HNL at pH 5.8. It may be postulated that the photoreactivation of SOD consists essentially in deprotonation of His-61 residue in the enzyme active site and subsequent recovery of imidazole bridge between copper and zinc which had been destroyed at low pH. Since many other enzymes possess similar copper-histidine structures in their active sites, one may expect diverse effects of red (laser) light on the enzyme activity.
Gorbatenkova
E A
EA
Vladimirov
Iu A
IuA
Paramonov
N V
NV
Azizova
O A
OA
D000818:Animals; D001665:Binding Sites / Q000528:radiation effects; D002417:Cattle; D003300:Copper / Q000032:analysis / Q000528:radiation effects; D004578:Electron Spin Resonance Spectroscopy; D004789:Enzyme Activation / Q000528:radiation effects; D004912:Erythrocytes / Q000201:enzymology / Q000528:radiation effects; D006639:Histidine / Q000032:analysis / Q000528:radiation effects; D006863:Hydrogen-Ion Concentration; D007834:Lasers; D011522:Protons; D013053:Spectrophotometry; D013482:Superoxide Dismutase / Q000032:analysis / Q000037:antagonists & inhibitors / Q000528:radiation effects*
D011522:Protons; D006639:Histidine; D003300:Copper; D013482:Superoxide Dismutase
Krasnyĭ svet geliĭ-neonovogo lazera reaktiviruet superoksiddismutazu.