Circulating T-lymphocyte sub-subsets and NK-like cells in renal transplant patients: patterns associated with rejection.
The patterns of circulating T sub-subset and NK-like cells were monitored in 87 renal transplant patients on a total of 122 occasions. Combinations of monoclonal antibodies were used in two-colour flow cytometric (FACS) analysis to define the major T helper/inducer and T suppressor/cytotoxic subsets and their activated counterparts, the T suppressor inducer and T suppressor effector sub-subsets, NK-like cells and their activated counterparts. In patients with rejection, the proportions of T suppressor inducer and T suppressor effector cells were less and the proportion of activated NK-like cells greater compared with cases exhibiting stable graft function, and these group differences were highly significant. Analysis with stepwise multivariate logistic regression showed that combinations of several functional/activated lymphocyte subsets allow a predictive diagnostic power which is superior to the use of single variables.
Hanås
E
E
Department of Surgery, University of Uppsala, Sweden.
Tötterman
T H
TH
Fellström
B
B
Bergström
R
R
Tufveson
G
G
D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis; D000943:Antigens, Differentiation; D000945:Antigens, Differentiation, T-Lymphocyte; D015415:Biomarkers / Q000097:blood; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens; D002469:Cell Separation; D005434:Flow Cytometry; D006084:Graft Rejection / Q000276:immunology*; D006085:Graft Survival / Q000276:immunology*; D006801:Humans; D016030:Kidney Transplantation; D007694:Killer Cells, Natural / Q000276:immunology*; D011961:Receptors, Fc / Q000032:analysis; D017452:Receptors, IgG; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000943:Antigens, Differentiation; D000945:Antigens, Differentiation, T-Lymphocyte; D015415:Biomarkers; D015704:CD4 Antigens; D016827:CD8 Antigens; D011961:Receptors, Fc; D017452:Receptors, IgG