title: Kinetics of cell replication in the uterine cervix. VIII. Quantitation of chronologic events in proliferating loci.
abstract: The frequency and the topographic distribution of DNA replicating cervical basal squamous cells (as deduced by 3H-thymidine in vivo administration up to 8 hours before sacrifice) was studied in 70,000 basal cells in 70 C57B1 mice. While the number of labelled basal cells/animal was similar in those injected only once, a significantly higher number of labelled basal cells was recorded in animals injected twice or three times. Following one single injection, 30% (8 h previously) and 31% (1 h previously) of the DNA replicating basal cells occurred in pairs or in loci of three, four or more labelled cells. After repeated injections, the rates were 49% for those animals injected twice before sacrifice and 47% for those injected three times. Thus repeated injections resulted in a significantly increased number of loci of three, four or more DNA replicating basal cells when compared to those injected only once. The present results suggest that there is a chronological asynchrony in the DNA replication of proliferating basal cells-loci of the uterine cervix.
authors.affiliation: Department of Pathology, Karolinska Institute, Stockholm, Sweden.
authors.forename: C A
authors.initials: CA
authors.lastname: Rubio
authors.forename: M
authors.initials: M
authors.lastname: Llatjos
authors.forename: B
authors.initials: B
authors.lastname: Nilsson
mesh_terms: D000818:Animals; D002455:Cell Division; D004261:DNA Replication; D004847:Epithelial Cells; D005260:Female; D007700:Kinetics; D051379:Mice; D008810:Mice, Inbred C57BL; D011865:Radioisotope Dilution Technique; D013936:Thymidine / Q000378:metabolism; D014599:Uterus / Q000166:cytology*
chemical_list: D013936:Thymidine