[NAD participation in protecting DNA from damaging factors].
It was found that autolytic degradation of DNA during the isolation of nuclei under conditions of an in situ stimulated NAD biosynthesis is decreased to 2.5% in comparison with 5.2% in control. The increase in the NAD-pyrophosphorylase activity and the intranuclear content of NAD was almost 3-fold, whereas the amount of the acid-soluble DNA material was decreased 2-fold. The degree of DNA damage was increased up to 23% after induction with ethidium bromide. Under these conditions a preliminary in vivo stimulation of NAD biosynthesis by nicotinamide diminished the amount of the acid-soluble material. The levels of intranuclear NAD as well as the NAD-pyrophosphorylase and poly-(ADP-riboso)polymerase activities were essentially affected.
Muliavko
N A
NA
Petik
A V
AV
Donchenko
G V
GV
D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology; D002645:Chickens; D002843:Chromatin / Q000378:metabolism; D004249:DNA Damage; D004996:Ethidium / Q000494:pharmacology; D008099:Liver / Q000201:enzymology; D009243:NAD / Q000096:biosynthesis / Q000378:metabolism*; D009536:Niacinamide / Q000494:pharmacology
D002843:Chromatin; D009243:NAD; D009536:Niacinamide; D004996:Ethidium
Uchastie NAD v predokhranenii DNK ot povrezhdaiushchikh faktorov.