A gene encoding the proteolipid subunit of Sulfolobus acidocaldarius ATPase complex.
An analysis of genes for the major two subunits of the membrane-associated ATPase from an acidothermophilic archaebacterium, Sulfolobus acidocaldarius, suggested that it belongs to a different ATPase family from the F1-ATPase (Denda, K., Konishi, J., Oshima, T., Date, T., and Yoshida, M. (1988) J. Biol. Chem. 263, 17251-17254). In the same operon of the above two genes we found a gene encoding a very hydrophobic protein of 101 amino acids (Mr = 10,362). A proteolipid was purified from the membranes of this bacteria in which partial amino acid sequences matched with the sequence deduced from the gene. Significant amino acid sequence homology and a similar hydropathy profile appeared when the sequence was compared with the 8-kDa proteolipid subunit of F0F1-ATPases. It is about 30 amino acids larger than the 8-kDa proteolipid and has a small (11-amino acid) repeat sequence. However, it is distinct from the 16-kDa proteolipid subunit of an eukaryotic vacuolar H+-ATPase (Mandel, M., Moriyama, Y., Hulmes, J.D., Pan, Y.-E., Nelson, H., and Nelson, N. (1988) Proc. Natl. Acad. Sci. U.S.A. 85,5521-5524).
Denda
K
K
Department of Life Science, Tokyo Institute of Technology, Yokohama Japan.
Konishi
J
J
Oshima
T
T
Date
T
T
Yoshida
M
M
D000251:Adenosine Triphosphatases / Q000235:genetics*; D000595:Amino Acid Sequence; D001105:Archaea / Q000201:enzymology* / Q000235:genetics; D001419:Bacteria / Q000201:enzymology*; D001483:Base Sequence; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000235:genetics / Q000648:ultrastructure; D008969:Molecular Sequence Data; D011510:Proteolipids / Q000235:genetics* / Q000648:ultrastructure; D012995:Solubility
D046911:Macromolecular Substances; D008565:Membrane Proteins; D011510:Proteolipids; D000251:Adenosine Triphosphatases