Promoters of the rolA, B, and C genes of Agrobacterium rhizogenesare differentially regulated in transgenic plants.
Chimeric genes containing the beta-glucuronidase reporter gene under the control of the rolA, B, and C promoters of Agrobacterium rhizogenes are expressed in a regulated manner in transgenic plants. The intergenic region separating the rolB and C genes represents a bidirectional promoter. This bidirectional promoter regulates transcription for both genes in a similar fashion in aerial organs of the plants, but in a distinct way in roots. Moreover, both rolB and C promoter activities differ from those characteristic of the rolA promoter. Thus, promoters of bacterial origin show differential expression in transgenic plants, and regulation of roI gene expression plays a role in the biological effects caused by the rolA, B, and C genes.
Schmülling
T
T
Max-Planck-Institut für Züchtungsforschung, Köln, Federal Republic of Germany.
Schell
J
J
Spena
A
A
D046508:Culture Techniques; D005470:Fluorometry; D015964:Gene Expression Regulation, Bacterial; D009928:Organ Specificity / Q000235:genetics; D030821:Plants, Genetically Modified / Q000166:cytology / Q000235:genetics / Q000382:microbiology*; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D012231:Rhizobium / Q000235:genetics*