Molecular cloning of plant transcripts encoding protein kinase homologs.
Oligonucleotides, corresponding to conserved regions of animal protein-serine/threonine kinases, were used to isolate cDNAs encoding plant homologs in the dicot bean (Phaseolus vulgaris L.) and the monocot rice (Oryzae sativa L.). The C-terminal regions of the deduced polypeptides encoded by the bean (PVPK-1) and rice (G11A) cDNAs, prepared from mRNAs of suspension cultures and leaves, respectively, contain features characteristic of the catalytic domains of eukaryotic protein-serine/threonine kinases, indicating that these cDNAs encode plant protein kinases. The putative catalytic domains are most closely related to cyclic nucleotide-dependent protein kinases and the protein kinase C family, suggesting the plant homologs may likewise transduce extracellular signals. However, outside these domains, PVPK-1 and G11A exhibit no homology either to each other or to regulatory domains of other protein kinases, indicating the plant homologs are modulated by other signals. PVPK-1 corresponds to a 2.4-kb transcript in suspension cultured bean cells. Southern blots of genomic DNA indicate that PVPK-1 and G11A correspond to single copy genes that form part of a family of related plant sequences.
Lawton
M A
MA
Plant Biology Laboratory, Salk Institute for Biological Studies, San Diego, CA 92138-9216.
Yamamoto
R T
RT
Hanks
S K
SK
Lamb
C J
CJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D002384:Catalysis; D003001:Cloning, Molecular; D000242:Cyclic AMP / Q000494:pharmacology; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D007887:Fabaceae; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D012275:Oryza; D010944:Plants / Q000201:enzymology / Q000235:genetics*; D010946:Plants, Medicinal; D011493:Protein Kinase C / Q000235:genetics; D011494:Protein Kinases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D004247:DNA; D000242:Cyclic AMP; D011494:Protein Kinases; D011493:Protein Kinase C