Histochemical alterations in the adult rat brain after X-ray irradiation: effects of O-(beta-hydroxyethyl)-rutosides.
X-ray-induced tissue damage in the brain of adult rats was investigated in the presence or absence of O-(beta-hydroxyethyl)-rutosides (OHR; active agents of Venoruton, Zyma-Blaes, München, FRG). Histochemical methods were used for the detection of glycogen (periodic acid-Schiff), acid mucopolysaccharides (Hale) and acid phosphatases (Gomori) by light microscopy. The tissue alterations were reduced after drug application in the dose range between 5 and 7.5 Gy (300 kV, 12 mA). Exposure of the animals to higher irradiation doses (10, 20 Gy) led to an inversion of the drug effect, now exerting pronounced tissue injury. For a possible explanation we discuss the inhibitory influence of rutosides (e.g. OHR) on the glycolytic pathway. Hence, a vital energy source of brain tissue could be impaired by the drug after reaching a threshold of 10 Gy.
Lehmann
F M
FM
Abteilung für Neuroanatomie, Anatomisches Institut der Universität Hamburg, BRD.
Lierse
W
W
Thiel
H J
HJ
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects; D001921:Brain / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D004734:Energy Metabolism / Q000187:drug effects; D005260:Female; D006003:Glycogen / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism; D006651:Histocytochemistry; D006896:Hydroxyethylrutoside / Q000494:pharmacology*; D008297:Male; D011833:Radiation Injuries, Experimental / Q000517:prevention & control; D051381:Rats; D011919:Rats, Inbred Strains; D012431:Rutin / Q000031:analogs & derivatives*
D006025:Glycosaminoglycans; D006896:Hydroxyethylrutoside; D012431:Rutin; D006003:Glycogen; D000135:Acid Phosphatase