[Inhibitory effect of recombinant human interferon gamma on human osteoblastic osteosarcoma cells (SaOS2)].
Recent studies have suggested that interleukin-1 or tumor necrosis factor-stimulated bone resorption is mediated by osteoclast-activating factor elaborated by osteoblastic cells. Since recombinant interferon gamma inhibits stimulation of bone resorption by these cytokines, we examined here the effects of recombinant human interferon gamma (rhIFN-G) on DNA synthesis and alkaline phosphatase (ALP) activity of a human osteoblastic osteosarcoma cell line, SaOS2, under preconfluent culture conditions. Addition of rhIFN-G to the cells markedly inhibited their DNA synthesis and ALP activity in a dose-dependent fashion. However, the inhibition was not dependent on the culture time. The highest inhibitory effect was observed in 10% serum-containing culture medium. The inhibitory effect on DNA synthesis was not eliminated by addition of indomethacin, a cyclooxygenase inhibitor. Furthermore, combination of rhIFN-G and recombinant human tumor necrosis factor alpha inhibited their DNA synthesis and the ALP activity in synergistic fashion. Therefore, these data suggest that rhIFN-G is a potent inhibitor for human osteoblastic cells.
Katayama
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H
Hanazawa
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D000469:Alkaline Phosphatase / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D010006:Osteoblasts / Q000187:drug effects*; D012516:Osteosarcoma / Q000201:enzymology*; D011994:Recombinant Proteins
D011994:Recombinant Proteins; D007371:Interferon-gamma; D004247:DNA; D000469:Alkaline Phosphatase