Effects of clindamycin and cefuroxime on leukocyte membrane receptors and function.
Normal human granulocytes and lymphocytes were preincubated in 0.5, 5, or 50 mg/l of clindamycin, and 5, 50, or 500 mg/l of cefuroxime. Incubation with clindamycin caused an increase in the proportion of granulocytes bearing receptors for the Fc portion of IgG (Fc gamma-R) and C3b (C3b-R). Random migration in capillary tubes was significantly decreased, but phagocytosis as measured by chemiluminescence was only slightly decreased. The proportion of lymphocytes bearing Fc gamma-R was decreased, while there was no effect on lymphocyte C3b-R percentage, nor on the proportion of granulocytes or lymphocytes bearing sheep erythrocyte (E) receptors. Preincubation of granulocytes in cefuroxime was not associated with changes in the proportion of receptor-bearing cells, except for a slight increase in Fc gamma-R-bearing lymphocytes at the lowest concentration tested. Tube migration was not affected but chemiluminescence was significantly decreased. Preincubation with clindamycin, although increasing the proportion of cells bearing phagocytosis-associated receptors, is thus not associated with an increased phagocytic ability, while cefuroxime incubation caused a decrease in chemiluminescence despite normal levels of receptor-positive cells.
Noess
A
A
Medical Department B, Haukeland Hospital, University of Bergen, Norway.
Hauge
B
B
Solberg
C O
CO
D000943:Antigens, Differentiation / Q000032:analysis; D002444:Cefuroxime / Q000494:pharmacology*; D002511:Cephalosporins / Q000494:pharmacology*; D002981:Clindamycin / Q000494:pharmacology*; D006801:Humans; D007962:Leukocytes / Q000187:drug effects* / Q000502:physiology; D008163:Luminescent Measurements; D010587:Phagocytosis / Q000187:drug effects; D011951:Receptors, Complement / Q000032:analysis; D017463:Receptors, Complement 3b; D011961:Receptors, Fc / Q000032:analysis; D017452:Receptors, IgG
D000943:Antigens, Differentiation; D002511:Cephalosporins; D011951:Receptors, Complement; D017463:Receptors, Complement 3b; D011961:Receptors, Fc; D017452:Receptors, IgG; D002981:Clindamycin; D002444:Cefuroxime