Heparin fragments regulate collagen phenotype and fibronectin synthesis in the skin of genetically diabetic mice.
The biosyntheses of interstitial collagens of type I and III and of fibronectin were studied in genetically diabetic KK mice, as compared to control C57 Black mice, as well as the effect of low-Mr heparin fragments (CY 222) on these biosyntheses. An increased production of type III collagen, as compared to type I collagen, could be demonstrated in explant cultures of KK mice skins. Fibronectin biosynthesis was also increased. In vivo treatment of KK mice with 1 mg/kg of CY 222 decreased the biosyntheses of type III collagen and of fibronectin to normal levels. These experiments suggest that low-Mr heparin fragments can modulate the expression of extracellular matrix macromolecules.
Asselot
C
C
Laboratoire de Biochimie du Tissu Conjonctif, UA CNRS 1174, faculté de Médecine, Université, Paris XII, Creteil, France.
Labat-Robert
J
J
Kern
P
P
D000818:Animals; D003094:Collagen / Q000096:biosynthesis* / Q000235:genetics; D003921:Diabetes Mellitus, Experimental / Q000235:genetics* / Q000378:metabolism; D005109:Extracellular Matrix; D005353:Fibronectins / Q000096:biosynthesis*; D006495:Heparin, Low-Molecular-Weight / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D009924:Organ Culture Techniques; D010434:Pepsin A; D010446:Peptide Fragments / Q000494:pharmacology; D010641:Phenotype; D012867:Skin / Q000187:drug effects / Q000378:metabolism*
D005353:Fibronectins; D006495:Heparin, Low-Molecular-Weight; D010446:Peptide Fragments; D003094:Collagen; D010434:Pepsin A