title: Mutational analysis of bovine papillomavirus E6 gene.
abstract: The bovine papillomavirus E6 gene can independently transform mouse C127 cells. To characterize E6 in greater detail, we created 16 site-directed mutations in E6, including substitution mutations in the cysteine codons of the four Cys-X-X-Cys motifs that are conserved in all papillomavirus E6 proteins. Proteins mutated in six of the seven cysteines tested, as well as those lacking the nonconserved C-terminus, were stable in transfected cells but were unable to induce morphological transformation, indicating that these amino acids play an important role in the function of E6.
authors.affiliation: Laboratory of Cellular Oncology, National Cancer Institute, Bethesda, Maryland 20892.
authors.forename: K H
authors.initials: KH
authors.lastname: Vousden
authors.forename: E J
authors.initials: EJ
authors.lastname: Androphy
authors.forename: J T
authors.initials: JT
authors.lastname: Schiller
authors.forename: D R
authors.initials: DR
authors.lastname: Lowy
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D052688:Bovine papillomavirus 1 / Q000235:genetics*; D002460:Cell Line; D002472:Cell Transformation, Viral; D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins / Q000235:genetics; D005814:Genes, Viral; D008667:Metalloproteins / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000235:genetics*; D011233:Precipitin Tests; D014157:Transcription Factors / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics* / Q000276:immunology
chemical_list: D004268:DNA-Binding Proteins; D008667:Metalloproteins; D014157:Transcription Factors; D014764:Viral Proteins