Production of a monoclonal antibody against C terminus of atrial natriuretic factor by in vitro immunization.
Spleen cells, sensitized in vitro with ANF-KLH conjugate, were fused with a non-producing mouse myeloma cell line, X63-Ag8.653. One of the isolated hybridomas secreted a monoclonal antibody which exhibited an association constant of 7.25 X 10(-9) M for ANF and recognized an epitope at the C-terminal of the synthetic rat ANF(101-126). Cross reactivity experiments with Auriculin B, Atriopeptin I and Atriopeptin II suggests Phe124-Arg125-Tyr126 in the epitope recognized by this antibody. The epitope appears to be similar to the portion of the hormone molecule essential for ANF binding to the B-receptor (high m.wt. receptor). This antibody may be useful for the generation of anti-idiotypic antibodies for the B-receptors.
Rathinavelu
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Department of Pharmacology and Toxicology, School of Pharmacy and Pharmacal Sciences, Purdue University, W. Lafayette, IN 47907.
Isom
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000918:Antibody Specificity; D009320:Atrial Natriuretic Factor / Q000276:immunology*; D004232:Diuretics / Q000276:immunology*; D005260:Female; D006433:Hemocyanins / Q000276:immunology; D006825:Hybridomas / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000276:immunology*; D051381:Rats
D000911:Antibodies, Monoclonal; D004232:Diuretics; D007074:Immunoglobulin G; D010446:Peptide Fragments; D009320:Atrial Natriuretic Factor; D006433:Hemocyanins; C043970:atrial natriuretic factor 26, rat; C032808:keyhole-limpet hemocyanin