[Infection of peripheral blood lymphocytes in sheep with bovine leukemia virus in vitro].
Continuous cultivation of peripheral blood lymphocytes from healthy sheep was carried out in vitro with the help of human recombinant interleukin-2. Lymphocytes were concurrently cultivated with the lethally X-rayed BLV-producing FLK culture cells. Electron microscopy and dot-blot hybridization established that sheep peripheral blood lymphocytes were infected with BLV and a full cycle of replication takes place in them. Infection of sheep leukocytes in vitro can be used to study the mechanisms of leukogenesis in vitro.
Banders
U T
UT
Meldraĭs
E I
EI
Sauka
E I
EI
Razena
L G
LG
Line
I E
IE
D000818:Animals; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D001909:Leukemia Virus, Bovine / Q000472:pathogenicity*; D007942:Leukemia, Experimental / Q000382:microbiology; D008214:Lymphocytes / Q000382:microbiology* / Q000648:ultrastructure; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis; D012190:Retroviridae / Q000472:pathogenicity*; D012756:Sheep
D012367:RNA, Viral; D004247:DNA
Infitsirovanie limfotsitov perifericheskoĭ ovets virusom leĭkoza krupnogo rogatogo skota in vitro.