Identification of Shiga-like toxin in Escherichia coli strains, etiological agents of diarrheal disease.
Shiga-like toxin presence, in 20 E. coli strains, etiological agents of diarrheal diseases, is studied by preparing extracts at +4 degrees C, in the presence of chloroform and by i.v. inoculation in mice. In 4 out of 20 strains, Shiga-like toxin in high titres was identified. Most of the strains presented an inconstant and variable production of Shiga-like toxin in comparison with Shigella dysenteriae type 1 (Shiga) reference strain. The authors also confirm the existence of Shiga-like toxin under 2 forms (neutralizable with Shiga antitoxic serum and non-neutralizable). The importance of the obtained results is further discussed from the point of view of pathogeny and diagnosis of the infections produced by these germs.
Meitert
T
T
Tonciu
M
M
Ciudin
L
L
Pencu
E
E
Mihai
I
I
Nicolescu
S
S
D000818:Animals; D001427:Bacterial Toxins / Q000302:isolation & purification* / Q000633:toxicity; D003967:Diarrhea / Q000209:etiology* / Q000382:microbiology; D004305:Dose-Response Relationship, Drug; D004768:Enterotoxins / Q000302:isolation & purification* / Q000633:toxicity; D004926:Escherichia coli; D004927:Escherichia coli Infections / Q000209:etiology* / Q000382:microbiology; D051379:Mice; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; D022603:Shiga Toxins; D012762:Shigella dysenteriae
D001427:Bacterial Toxins; D004768:Enterotoxins; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; D022603:Shiga Toxins