Identification of enhancer elements in the upstream region of the nuclear photosynthetic gene ST-LS1.
The nuclear gene ST-LS1 from potato encodes a 10-kilodalton protein that is a component of the oxygen-evolving complex of photosystem II. Analysis of the expression of a reporter gene driven by chimeric promoters, consisting of ST-LS1 upstream sequences and a truncated cauliflower mosaic virus 35S promoter, suggests that a strong positive regulatory element is located between position -345 and -261, whereas both the region -261 to +11 and the more upstream region -1600 to -530 are devoid of autonomous strong positive elements detectable by this approach. The ST-LS1 upstream region contains redundant elements conferring light-regulated and organ-specific expression, one of them being located between position -130 and +11. In addition, enhancer-like sequences conferring light-regulated as well as organ-specific expression to heterologous promoters were identified. These sequences are functional not only when located 5'-upstream of the coding region but also when placed 3'-downstream of the polyadenylation signal, thus representing one of the first examples of a plant gene-derived enhancer being able to induce a truncated heterologous promoter from a position 3'-downstream of the transcription unit.
Stockhaus
J
J
Institut für Genbiologische Forschung Berlin GmbH, Federal Republic of Germany.
Schell
J
J
Willmitzer
L
L
D002467:Cell Nucleus / Q000378:metabolism; D004742:Enhancer Elements, Genetic / Q000235:genetics*; D005786:Gene Expression Regulation / Q000235:genetics* / Q000502:physiology; D009029:Mosaic Viruses / Q000235:genetics; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics*; D045332:Photosystem II Protein Complex; D030821:Plants, Genetically Modified / Q000235:genetics*; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics; D011198:Solanum tuberosum / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics
D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D011993:Recombinant Fusion Proteins