title: The major type-1 protein phosphatase catalytic subunits are the same gene products in rabbit skeletal muscle and rabbit liver.
abstract: The catalytic subunit of protein phosphatase-1 (PP1) isolated from rabbit liver had the same electrophoretic mobility as, and yielded peptide maps identical to those of the 33 kDa form of rabbit skeletal muscle PP1. The predicted amino-acid sequences of PP1 obtained from three rabbit liver cDNA clones were identical to that of PP1 alpha from rabbit skeletal muscle. These findings suggest that the distinctive substrate specificities and regulatory properties of hepatic and skeletal muscle type-1 protein phosphatases are not conferred by the catalytic subunits themselves, but by regulatory subunits that are complexed to the catalytic subunits in vivo.
authors.affiliation: Department of Biochemistry, Medical Sciences Institute, The University, Dundee, U.K.
authors.forename: P T
authors.initials: PT
authors.lastname: Cohen
authors.forename: D L
authors.initials: DL
authors.lastname: Schelling
authors.forename: O B
authors.initials: OB
authors.lastname: da Cruz e Silva
authors.forename: H M
authors.initials: HM
authors.lastname: Barker
authors.forename: P
authors.initials: P
authors.lastname: Cohen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005796:Genes; D008099:Liver / Q000201:enzymology*; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009132:Muscles / Q000201:enzymology*; D009928:Organ Specificity; D010449:Peptide Mapping; D010749:Phosphoprotein Phosphatases / Q000235:genetics* / Q000302:isolation & purification; D054645:Protein Phosphatase 1; D011817:Rabbits; D015183:Restriction Mapping
chemical_list: D046911:Macromolecular Substances; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1