The fertilization and cleavage rates of eggs recovered from the cul-de-sac.
Five hundred ninety-one oocytes were laparoscopically recovered in 93 consecutive stimulated cycles. Fifty-three of the 591 oocytes were collected from the cul-de-sac in 39 cycles. The cul-de-sac-originated oocytes had fertilization and cleavage rates of 72 and 79%, respectively; these rates were similar to those achieved in oocytes originating from follicles. A significant decrease in the fertilization rate of cul-de-sac-originated oocytes was noticed in oocytes collected from the cul-de-sac 60 minutes or more after the beginning of the procedure. Oocytes collected from the cul-de-sac yielded 21% of the embryos transferred in cycles where some of the oocytes were recovered from the cul-de-sac. These findings indicate that repeated aspirations of the cul-de-sac may increase the number of embryos transferred.
Dirnfeld
M
M
Department of Obstetrics and Gynecology, Carmel Hospital, Haifa, Israel.
Weisman
Z
Z
Sorokin
Y
Y
Sheinfeld
M
M
Lissak
A
A
Abramovici
H
H
D001826:Body Fluids / Q000166:cytology; D002469:Cell Separation / Q000379:methods*; D004312:Douglas' Pouch / Q000166:cytology*; D004624:Embryo Transfer; D005314:Embryonic and Fetal Development; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D010535:Laparoscopy; D009865:Oocytes / Q000166:cytology*; D006080:Ovarian Follicle / Q000166:cytology; D011247:Pregnancy; D013396:Suction