Estrogen can regulate the cell cycle in the early G1 phase of yeast by increasing the amount of adenylate cyclase mRNA.
The effects of beta-estradiol (estrogen; a minor component of yeast cells) on S. cerevisiae cells in the G0 and G1 phases were examined. Results showed that estrogen stimulated the recovery of growth from G0 arrest induced by nutrient limitation or ts mutation of cdc35 (adenylate cyclase) in the early G1 phase, and inhibited entry into the resting G0 phase by increasing the intracellular cAMP level. However, estrogen had no effect on late G1 arrest induced by the alpha factor or ts mutation of cdc36. Estrogen was found to lead to higher steady-state levels of adenylate cyclase mRNA but not to affect the expression of the RAS1 and RAS2 genes, although these can also alter the intracellular cAMP level. These results suggest that estrogen influences the cell cycle of yeast in the early G1 phase by controlling the level of cAMP through the increase of adenylate cyclase mRNA.
Tanaka
S
S
Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
Hasegawa
S
S
Hishinuma
F
F
Kurata
S
S
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000450:Aldosterone / Q000494:pharmacology; D000906:Antibodies / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects*; D000242:Cyclic AMP / Q000378:metabolism; D004967:Estrogens / Q000502:physiology*; D005656:Fungal Proteins / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D007399:Interphase; D012333:RNA, Messenger / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology*; D029701:Saccharomyces cerevisiae Proteins; D013739:Testosterone / Q000031:analogs & derivatives / Q000494:pharmacology; D013761:Tetrahydrocortisone / Q000494:pharmacology; D018631:ras Proteins
D000906:Antibodies; D004967:Estrogens; D005656:Fungal Proteins; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D013739:Testosterone; D000450:Aldosterone; C013915:boldenone; D013761:Tetrahydrocortisone; D000242:Cyclic AMP; C489014:RAS1 protein, S cerevisiae; C489015:RAS2 protein, S cerevisiae; D018631:ras Proteins; D000262:Adenylyl Cyclases