Cell electroporation is a highly efficient method for introducing restriction endonucleases into cells.
Restriction endonucleases that make either blunt- or cohesive-end DNA double-strand breaks can induce chromosome aberrations. We have used cell electroporation with great success to permeabilize Chinese hamster ovary cells for the introduction of restriction enzymes. The introduction of restriction enzymes by this method resulted in extremely high frequencies (greater than 90%) of aberrant metaphase cells and also a dramatic decrease in cell survival, as measured by subsequent colony formation. Cell electroporation by itself caused no increase in aberrant chromosomes and had only a slight effect on cell survival.
Winegar
R A
RA
Laboratory of Radiobiology and Environmental Health, University of California, San Francisco 94143-0750.
Phillips
J W
JW
Youngblom
J H
JH
Morgan
W F
WF
D000818:Animals; D002460:Cell Line; D002463:Cell Membrane Permeability; D002470:Cell Survival; D002869:Chromosome Aberrations; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004558:Electric Stimulation / Q000379:methods
D004262:DNA Restriction Enzymes