title: Physicochemical properties of 57 kDa thyroid hormone binding protein from rat liver nuclei.
abstract: The nuclear thyroid hormone binding protein (NTHB) with the molecular weight of 57 kDa was obtained from rat liver nuclear extracts by using HPLC and DEAE-Sephadex A-25 ion exchange chromatography methods. Fluorescein isothiocyanate-labeled 3,5,3'-triiodo-L-thyronine (F-T3) was used as a fluorescent probe to identify the hormone binding protein. Purified NTHB has a single binding site for T3 with the apparent binding constant of (3.3 +/- 0.7) X 10(8) M-1. NTHB is an acidic protein with a pI of 5.0. The secondary structure of NTHB is characterized by about 42% helical and 18% beta-structure from CD measurements.
authors.affiliation: Faculty of Pharmaceutical Sciences, Kinki University, Osaka.
authors.forename: N
authors.initials: N
authors.lastname: Okabe
authors.forename: K
authors.initials: K
authors.lastname: Goto
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000302:isolation & purification / Q000502:physiology*; D002467:Cell Nucleus / Q000502:physiology*; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005456:Fluorescent Dyes; D015200:Fluoroimmunoassay; D008099:Liver / Q000648:ultrastructure*; D008297:Male; D008565:Membrane Proteins / Q000302:isolation & purification / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D013861:Thiocyanates; D013963:Thyroid Hormones; D014284:Triiodothyronine; D000097810:Thyroid Hormone-Binding Proteins
chemical_list: D002352:Carrier Proteins; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005456:Fluorescent Dyes; D008565:Membrane Proteins; D013861:Thiocyanates; D013963:Thyroid Hormones; D014284:Triiodothyronine; D000097810:Thyroid Hormone-Binding Proteins