Neonatal suppression with anti-Ia antibody. III. In vivo responses to the type 2 antigen TNP-Ficoll.
We studied the response to thymus-independent type 2 (type 2) Ag in mice suppressed from birth with anti-Ia antibody. Although these mice have significantly reduced numbers of surface IgM+ cells and reduced or absent levels of Ia-restricted Th cell activity, their IgM antibody response to the type 2 Ag TNP-Ficoll was unaffected whereas that to the prototypic thymus-dependent Ag SRBC was predictably eliminated. These data suggest that an in vivo antibody response can be made to type 2 Ag in the absence of Ia-dependent cellular interactions. The surface IgM+IgD-Ia- B cells that are found in the anti-Ia antibody-suppressed mouse may represent an expanded population of Ia-independent, type 2 Ag-sensitive B cells normally present as a smaller proportion of the splenic lymphocyte population. Thymus-dependent responses, which have been shown to have an absolute requirement for an Ia-dependent interaction, are absent in these animals.
Fultz
M
M
Department of Medicine, Uniformed Services University of the Health Sciences, Bethesda, MD 20814-4799.
Carman
J
J
Finkelman
F D
FD
Mond
J J
JJ
D000818:Animals; D000831:Animals, Newborn / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000008:administration & dosage*; D000955:Antigens, T-Independent / Q000008:administration & dosage* / Q000276:immunology; D001402:B-Lymphocytes / Q000378:metabolism; D002450:Cell Communication; D005362:Ficoll / Q000008:administration & dosage* / Q000031:analogs & derivatives / Q000276:immunology; D006462:Hemolytic Plaque Technique; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D007166:Immunosuppressive Agents / Q000008:administration & dosage*; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008809:Mice, Inbred C3H; D009578:Nitrobenzenes / Q000008:administration & dosage*; D011134:Polysaccharides / Q000008:administration & dosage*; D014301:Trinitrobenzenes / Q000008:administration & dosage* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000955:Antigens, T-Independent; D000949:Histocompatibility Antigens Class II; D007166:Immunosuppressive Agents; D009578:Nitrobenzenes; D011134:Polysaccharides; C020529:TNP-ficoll; D014301:Trinitrobenzenes; D005362:Ficoll