title: Cyclic AMP- and cytochalasin B-induced arborization in cultured aortic smooth muscle cells: its cytopharmacological characterization.
abstract: The present study analyzed effects of dibutyryl cyclic AMP (DB-cAMP) and cytochalasin B (CB) on the morphology of cultured aortic smooth muscle cells (SMC) from rat using phase-contrast microscopy, scanning electron microscopy, and fluorescence staining of actin filaments by the NBD-phallacidin method. The exposure of SMC to each of these agents led to rapid, extensive, and reversible (within 1-2 h of drug withdrawal) changes in their morphology including cytoplasmic arborization (stellation). The latter was preceded by (i) marginal membrane ruffles (DB-cAMP) and (ii) increased zeiotic activity (CB), which were visible within 20 min of the exposure, followed (30-90 min incubation) by a centripetal retraction of the cytoplasm and progressive development of complete or partial arborization. Further, the effects of substances interfering with the assembly-disassembly of microtubules (colchicine, taxol, lidocaine) on DB-cAMP- and CB-induced arborization were studied. None of these agents antagonized CB-induced morphological changes. Colchicine, but not lumicolchicine, taxol, or lidocaine (in a short-term study) prevented DB-cAMP-induced arborization. Taxol added to cell cultures for 24 h promoted DB-cAMP-induced arborization. Both DB-cAMP and CB resulted in the disintegration of actin filaments. The present data suggest that the arborization of cultured aortic SMC is a cytoskeleton-based process involving stabilization of microtubules and disintegration of actin filaments. Our study also suggests that the SMC arborization may represent an in vitro case of SMC stellation found in situ.
authors.affiliation: Department of Pathology, Shimane Medical University, Izumo, Japan.
authors.forename: G N
authors.initials: GN
authors.lastname: Chaldakov
authors.forename: T
authors.initials: T
authors.lastname: Nabika
authors.forename: Y
authors.initials: Y
authors.lastname: Nara
authors.forename: Y
authors.initials: Y
authors.lastname: Yamori
mesh_terms: D000199:Actins / Q000032:analysis / Q000502:physiology; D000818:Animals; D001011:Aorta / Q000166:cytology; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000494:pharmacology*; D003571:Cytochalasin B / Q000494:pharmacology*; D003599:Cytoskeleton / Q000032:analysis / Q000502:physiology; D007545:Isoproterenol / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D008870:Microtubules / Q000502:physiology; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D051381:Rats; D011921:Rats, Inbred WKY
chemical_list: D000199:Actins; D003571:Cytochalasin B; D000242:Cyclic AMP; D007545:Isoproterenol