title: Detection of IgA antibodies against human papillomavirus in cervical secretions from patients with cervical intraepithelial neoplasia.
abstract: Although human papillomavirus (HPV) is involved in the etiology of cervical carcinoma, there are no cervical carcinoma-specific HPV serologic tests available. We investigated the presence of broadly cross-reactive IgA antibodies to papillomavirus (PV) in cervico-vaginal secretions from patients with condylomata and cervical intraepithelial neoplasia (CIN). Purified bovine PV (BPV) virions were used as antigen in an enzyme-linked immunosorbent assay (ELISA). Forty-two women whose ages ranged from 20 to 50 participated in the study. Eight of 9 patients with CIN had IgA antibodies against PV in their cervical secretions. Three of 9 patients with koilocytosis and condylomas but no CIN had IgA antibodies to PV. Six of 24 women with normal Pap-smear and colposcopy also had IgA antibodies against PV in their cervical secretions. The proportion of IgA-positive cervical secretions was significantly higher in the CIN group than in the normal group (p less than 0.005). Our data suggest that IgA antibodies to PV may be a useful marker for CIN.
authors.affiliation: Department of Medical Microbiology, University of Lund, Sweden.
authors.forename: L
authors.initials: L
authors.lastname: Dillner
authors.forename: Z
authors.initials: Z
authors.lastname: Bekassy
authors.forename: N
authors.initials: N
authors.lastname: Jonsson
authors.forename: J
authors.initials: J
authors.lastname: Moreno-Lopez
authors.forename: J
authors.initials: J
authors.lastname: Blomberg
mesh_terms: D000328:Adult; D000914:Antibodies, Viral / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis; D008875:Middle Aged; D008970:Molecular Weight; D065006:Papanicolaou Test; D027383:Papillomaviridae / Q000276:immunology*; D002583:Uterine Cervical Neoplasms / Q000382:microbiology*; D014626:Vaginal Smears
chemical_list: D000914:Antibodies, Viral; D007070:Immunoglobulin A; D007074:Immunoglobulin G