Distribution of omega-3 and omega-6 fatty acids in the ether- and ester-linked phosphoglycerides from tissues of the rainbow trout, Salmo gairdneri.
1. Data presented here demonstrate that polyunsaturated fatty acids in the phospholipids of rainbow trout tissues are compartmentalized differently than in mammalian tissues. 2. We have determined the distribution of omega-3 (n-3) and omega-6 (n-6) fatty acids in the alkyl-, alk-1-enyl-, and diacyl- subclasses of phosphatidylcholines (PC), phosphatidyl-ethanolamines (PE), phosphatidylinositols (PI), and phosphatidylserines (PS) from gill, kidney and spleen of rainbow trout. 3. Alkyl-linked PC and alk-1-enyl-linked PE were the most abundant ether-containing phospholipids, amounting to 10-15% of each class; no ether-linked PI or PS was detected. 4. C20:4 n-6 was found in high concentrations only in PI; the n-3 fatty acids were found in highest concentration in the ether-linked phospholipids as compared with the diacyl subclasses and C20:5 n-3 was especially prevalent in 1-O-alk-1'-enyl-2-acyl-sn-glycero-3-phosphoethanolamine and C22:6 n-3 was prevalent in PS.
Turner
M R
MR
Mountain Aquaculture Research Center, Western Carolina University, Cullowhee, NC 28723.
Leggett
S L
SL
Lumb
R H
RH
D000818:Animals; D015525:Fatty Acids, Omega-3 / Q000378:metabolism; D043371:Fatty Acids, Omega-6; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D005880:Gills / Q000378:metabolism; D005994:Glycerophosphates / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D010955:Plasmalogens / Q000378:metabolism; D012487:Salmonidae / Q000378:metabolism*; D013154:Spleen / Q000378:metabolism; D014018:Tissue Distribution; D014337:Trout / Q000378:metabolism*
D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6; D005231:Fatty Acids, Unsaturated; D005994:Glycerophosphates; D010743:Phospholipids; D010955:Plasmalogens; C020791:phosphatidal ethanolamines