title: Histone acetylation reduces nucleosome core particle linking number change.
abstract: Nucleosome core particles differing in their levels of histone acetylation have been formed on a closed circular DNA that contains a tandemly repeated 207 bp nucleosome positioning sequence. The effect of acetylation on the linking number per nucleosome particle has been determined. With increasing levels of acetylation, the negative linking number change per nucleosome decreases from -1.04 +/- 0.08 for control to -0.82 +/- 0.05 for highly acetylated nucleosomes. These results indicate that histone acetylation has the ability to release negative supercoils previously constrained by nucleosomes into a closed chromatin loop and in effect function as a eukaryotic gyrase.
authors.affiliation: Department of Biological Chemistry, School of Medicine, University of California, Davis 95616.
authors.forename: V G
authors.initials: VG
authors.lastname: Norton
authors.forename: B S
authors.initials: BS
authors.lastname: Imai
authors.forename: P
authors.initials: P
authors.lastname: Yau
authors.forename: E M
authors.initials: EM
authors.lastname: Bradbury
mesh_terms: D000107:Acetylation; D001482:Base Composition; D002875:Chromosomes / Q000032:analysis; D004250:DNA Topoisomerases, Type II / Q000502:physiology; D004270:DNA, Circular / Q000378:metabolism / Q000648:ultrastructure; D004278:DNA, Superhelical / Q000032:analysis; D008040:Genetic Linkage; D006367:HeLa Cells; D006657:Histones / Q000378:metabolism*; D006801:Humans; D009707:Nucleosomes / Q000378:metabolism* / Q000648:ultrastructure; D010957:Plasmids; D012341:RNA, Ribosomal, 5S / Q000235:genetics
chemical_list: D004270:DNA, Circular; D004278:DNA, Superhelical; D006657:Histones; D009707:Nucleosomes; D012341:RNA, Ribosomal, 5S; D004250:DNA Topoisomerases, Type II