Determination of lovastatin acid in serum by gas chromatography/mass spectrometry.
The acid form of lovastatin, an HMG-CoA reductase inhibitor, was analyzed by gas chromatography/negative-ion chemical ionization mass spectrometry after derivatization with pentafluorobenzyl bromide and bis-(trimethylsilyl)trifluoroacetamide (BSTFA). Mass spectrometry of this derivative produced a dominant [M-181]- ion under chemical ionization conditions using ammonia as the reagent gas. The limit of detection was approximately 2 pg injected on column.
Wang-Iverson
D
D
Squibb Institute for Medical Research, Analytical Research and Development, New Brunswick, New Jersey 08903.
Ivashkiv
E
E
Jemal
M
M
Cohen
A I
AI
D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D008148:Lovastatin / Q000031:analogs & derivatives* / Q000097:blood
C040295:mevinolinic acid; D008148:Lovastatin