title: Truncated forms of human complement factor H.
abstract: By the use of Western-blot analyses with polyclonal anti-(Factor H) antibodies, two low-Mr protein species of Mr 41,000 and 37,000 under non-reducing conditions and 43,000 and 40,000 under reducing conditions are consistently detected together with the well-known 155,000-Mr Factor H in human plasma and serum. These two additional species are also found in plasma, urine and synovial fluids. The 41,000-Mr species but not the 37,00-Mr species is detected by a monoclonal anti-(Factor H) antibody directed at the N-terminal part of Factor H. The 37,000-Mr species but not the 41,000-Mr species is detected by a monoclonal anti-(Factor H) antibody directed at the C-terminal part of Factor H. The 41,000-Mr and 37,000-Mr species are different from the well-characterized 36,000-Mr N-terminal tryptic fragment of Factor H. They are likely to represent translational products of the short Factor H mRNA species of 1.8 kb and 1.2-1.5 kb occurring in human liver that we have recently described.
authors.affiliation: I.N.S.E.R.M. Unité 78, Bois-Guillaume, France.
authors.forename: M
authors.initials: M
authors.lastname: Fontaine
authors.forename: M J
authors.initials: MJ
authors.lastname: Demares
authors.forename: V
authors.initials: V
authors.lastname: Koistinen
authors.forename: A J
authors.initials: AJ
authors.lastname: Day
authors.forename: C
authors.initials: C
authors.lastname: Davrinche
authors.forename: R B
authors.initials: RB
authors.lastname: Sim
authors.forename: J
authors.initials: J
authors.lastname: Ripoche
mesh_terms: D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D003180:Complement C3b Inactivator Proteins / Q000032:analysis*; D017242:Complement Factor H; D006801:Humans; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis
chemical_list: D000911:Antibodies, Monoclonal; C493495:CFH protein, human; D003180:Complement C3b Inactivator Proteins; D010446:Peptide Fragments; D017242:Complement Factor H