Enhancement of Epstein-Barr virus/C3d receptor (EBV/C3dR or CR2) and nuclear p120 ribonucleoprotein phosphorylation by specific EBV/C3dR ligands in subcellular fractions of the human B lymphoma cell line, Raji.
We present herein the first evidence that interaction of specific EBV/C3dR ligands, as human C3bi/C3d and anti-EBV/C3dR MoAb, with EBV/C3dR enhanced significantly, in a dose dependent process, phosphorylation of EBV/C3dR and p120 RNP present in subcellular fractions, as purified plasma membranes and nuclei, of the human B lymphoma cell line, Raji. The use of kinase effectors allowed to detect some of the kinases involved in these phosphorylations. Pp60src-like phosphotyrosine kinase and protein kinase C were involved in the phosphorylation of plasma membrane or nuclear EBV/C3dR. An additional calcium/calmodulin-dependent kinase was also involved in nuclear EBV/C3dR phosphorylation. P120 RNP phosphorylation was under the control of protein kinase C and of CaCl2/Calmodulin-dependent kinase but also of casein kinase II.
Delcayre
A X
AX
Immunochimie des Antigènes de Membrane et des Interactions Cellulaires, INSERM U.23, Hôpital Saint-Antoine, Paris, France.
Fiandino
A
A
Lyamani
F
F
Barel
M
M
Frade
R
R
D002051:Burkitt Lymphoma; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism*; D004854:Herpesvirus 4, Human / Q000502:physiology; D006801:Humans; D007700:Kinetics; D008024:Ligands; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D017464:Receptors, Complement 3d; D011991:Receptors, Virus / Q000302:isolation & purification / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000302:isolation & purification / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism
D008024:Ligands; D017464:Receptors, Complement 3d; D011991:Receptors, Virus; D012261:Ribonucleoproteins; D011494:Protein Kinases