IFN-mediated differential regulation of the expression of HLA-B7 and HLA-A3 class I genes.
The regulation by IFN of the expression of HLA-B7 and HLA-A3 class I molecules was studied in Jurkat human T lymphoma cells, HHK EBV-transformed human B lymphocytes, and murine HLA-B7 HLA-A3 co-transfected L fibroblasts. Jurkat cells express constitutively low level of HLA class I molecules and treatment with human IFN resulted in preferential increase of the expression of HLA-B7 molecules, the expression of the HLA-A3 molecules being relatively unchanged. Similar treatment of HHK cells, which express constitutively large amount of HLA class I molecules, resulted in a marginal increase of the expression of both HLA-B7 and HLA-A3 molecules. HLA-B7 HLA-A3 co-transfected L cells express relatively low level of HLA class I molecules, expression of both however was significantly increased after treatment with murine INF-alpha, the augmentation being more accentuated for HLA-B7 molecules. In all cases, variations of cell surface expression were related to parallel modifications of the level of HLA-B7 and HLA-A3 RNA transcripts. Important nucleotide differences exist between the IFN consensus sequences associated with the HLA-B7 and HLA-A3 class I genes. Using oligonucleotides corresponding to these sequences two patterns of retarded bands were observed by the gel mobility shift assay, suggesting that the IFN-mediated differential regulation of the expression of the HLA-B7 and HLA-A3 genes could be due to different nuclear regulatory factors.
Hakem
R
R
Centre d'Immunologie, INSERM-CNRS, Marseille-Luminy, France.
Le Bouteiller
P
P
Barad
M
M
Trujillo
M
M
Mercier
P
P
Wietzerbin
J
J
Lemonnier
F A
FA
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D002461:Cell Line, Transformed; D004268:DNA-Binding Proteins / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D015234:HLA-A Antigens / Q000235:genetics*; D015791:HLA-A3 Antigen; D015235:HLA-B Antigens / Q000235:genetics*; D015793:HLA-B7 Antigen; D004854:Herpesvirus 4, Human; D006801:Humans; D007370:Interferon Type I / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology*; D007371:Interferon-gamma / Q000235:genetics / Q000494:pharmacology*; D007739:L Cells; D008223:Lymphoma / Q000235:genetics / Q000276:immunology; D051379:Mice; D011401:Promoter Regions, Genetic / Q000187:drug effects; D013601:T-Lymphocytes / Q000276:immunology; D014158:Transcription, Genetic; D014162:Transfection
D000276:Adjuvants, Immunologic; D004268:DNA-Binding Proteins; D015234:HLA-A Antigens; D015791:HLA-A3 Antigen; D015235:HLA-B Antigens; D015793:HLA-B7 Antigen; D007370:Interferon Type I; D007371:Interferon-gamma