Androgen receptors, androgen-dependent proliferation, and 5 alpha-reductase activity of small-cell lung cancer cell lines.
We investigated the influence of testosterone on binding to established small-cell lung cancer (SCLC) cell lines and were able to show specific high-affinity and low-capacity binding sites in some cell lines with a typical receptor size, using sucrose density gradient centrifugation. In addition, we demonstrated marked growth stimulation with testosterone and dehydrotestosterone using different androgen-receptor-positive small-cell lung cancer cell lines. This growth stimulation could be counteracted by the addition of anti-androgens like cyproterone acetate or flutamide. The presence of 5 alpha-reductase activity, 17 beta-hydroxysteroid-dehydrogenase and 3 alpha-hydroxysteroid-dehydrogenase activities could be demonstrated, suggesting testosterone metabolism of small-cell lung cancer cell lines.
Maasberg
M
M
Department of Medicine, University of Marburg, FRG.
Rotsch
M
M
Jaques
G
G
Enderle-Schmidt
U
U
Weehle
R
R
Havemann
K
K
D000726:Androgen Antagonists / Q000494:pharmacology; D000728:Androgens / Q000378:metabolism / Q000494:pharmacology*; D018288:Carcinoma, Small Cell / Q000378:metabolism* / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D042944:Cholestenone 5 alpha-Reductase; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D010088:Oxidoreductases / Q000032:analysis / Q000378:metabolism*; D011869:Radioligand Assay; D011944:Receptors, Androgen / Q000187:drug effects / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014407:Tumor Cells, Cultured
D000726:Androgen Antagonists; D000728:Androgens; D011944:Receptors, Androgen; D014316:Tritium; D010088:Oxidoreductases; D042944:Cholestenone 5 alpha-Reductase; D013936:Thymidine