Role of bacteriophage Mu C protein in activation of the mom gene promoter.
The phage Mu C gene product is a specific activator of Mu late gene transcription, including activation of the mom operon. Fusion of the C gene to the efficient translation initiation region of the Escherichia coli atpE gene allowed significant overproduction of C protein, which was subsequently purified and assayed for DNA binding by gel retardation and nuclease footprinting techniques. C protein binds to a site immediately upstream of the -35 region both of the mom promoter and the related phage D108 mod promoter. The location of the mom promoter has been determined by primer extension. Upstream deletions extending more than 3 base pairs into the C-binding site abolished activation of the mom promoter in vivo. In vitro binding of C was not significantly affected by DNA methylation. A second, C-dependent promoter was identified just downstream of the C coding region; comparison with the mom promoter revealed common structural elements.
Bölker
M
M
Institut für Genbiologische Forschung Berlin GmbH, Federal Republic of Germany.
Wulczyn
F G
FG
Kahmann
R
R
D000595:Amino Acid Sequence; D010583:Bacteriophage mu / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D002874:Chromosome Mapping; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000302:isolation & purification; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D014764:Viral Proteins / Q000235:genetics* / Q000302:isolation & purification
D004268:DNA-Binding Proteins; D014764:Viral Proteins