[Method and result of oxygen addition in the culture process of Pseudomonas aeruginosa].
Pseudomonas aeruginosa septicemia are frequent complications of patients with major burns. To evaluate more rapid culture methods for diagnosis, we have established a new culture method of pseudomonas aeruginosa by oxygen addition. The results of vitro study showed that the growth time of pseudomonas aeruginosa was 8 to 12 hours earlier by the new culture method than by the ordinary method, when pseudomonas aeruginosa were incubated in broth at 37 degrees C to achieve approximately 50 to 65 colony-forming units/bottle. A total of 60 samples was tested when each culture bottle was added approximately 2 to 10 colony-forming units. The percentage of positive culture was 75% by venting oxygen addition method and 33.3% by ordinary method. The result of assay of 26 blood samples from patients with major burns showed that the percentage of positive blood cultures was 69.2% by the new method and 42.3% by the ordinary method. The culture time of the new method was 4 to 12 hours earlier than the ordinary method. This technique may lead to a greater chance of recovery and rapid detection of pseudomonas aeruginosa from blood samples.
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D001431:Bacteriological Techniques; D002056:Burns / Q000097:blood / Q000382:microbiology; D003470:Culture Media; D006801:Humans; D010100:Oxygen; D011550:Pseudomonas aeruginosa / Q000254:growth & development*
D003470:Culture Media; D010100:Oxygen