title: Molecular cloning of a family of plant genes encoding a protein homologous to plasma membrane H+-translocating ATPases.
abstract: cDNA clones from Nicotiana plumbaginifolia have been isolated by hybridization to a yeast H+-ATPase gene. The largest one encodes a polypeptide (PMA2) of 956 amino acid residues which exhibits a homology of 73% with a limited protein sequence obtained from purified oat plasma membrane H+-ATPase (Schaller and Sussman, Plant Physiol. 86, 512-516, 1988) and an 82% homology with the Arabidopsis thaliana pma gene (Harper et al., Proc. Natl. Acad. Sci. USA 86, 1234-1238). It is therefore concluded that the N. plumbaginifolia pma2 gene encodes a plasma membrane H+-ATPase. Southern blot hybridization indicates that the plant pma2 gene belongs to a multigene family. Partial sequences of cDNA clones show that at least three pma genes are expressed in root cells.
authors.affiliation: Unité de Biochimie Physiologique, Université Catholique de Louvain, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Boutry
authors.forename: B
authors.initials: B
authors.lastname: Michelet
authors.forename: A
authors.initials: A
authors.lastname: Goffeau
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002462:Cell Membrane / Q000201:enzymology*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D010944:Plants / Q000235:genetics*; D010947:Plants, Toxic; D006180:Proton-Translocating ATPases / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014026:Nicotiana
chemical_list: D004247:DNA; D006180:Proton-Translocating ATPases