title: Increased level of mitochondrial gene expression in polyps of familial polyposis coli patients.
abstract: To elucidate the mechanism of polyp formation in familial polyposis coli patients, cDNA clones, expressed differently between normal colonic mucosae and polyps, were screened. Three polyp-derived cDNA clones showed increased levels of transcription under neoplastic conditions. Among them, clone P6 was determined to be derived from the mtDNA encoding parts of subunit 1 of NADH dehydrogenase and 16S rRNA. The number of copies and restriction enzyme patterns of the mtDNA did not vary in either normal or neoplastic tissues, and a level of different mitochondrial mRNA was increased in the same proportion, suggesting a change in the rate of synthesis and/or degradation of the mtRNA. No cDNA clone which was expressed higher in normal colonic mucosae than in polyps was isolated from the cDNA library derived from normal mucosae.
authors.affiliation: Department of Hygiene, Gunma University, School of Medicine, Maebashi, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Yamamoto
authors.forename: S
authors.initials: S
authors.lastname: Horai
authors.forename: Y
authors.initials: Y
authors.lastname: Yuasa
mesh_terms: D011125:Adenomatous Polyposis Coli / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D004272:DNA, Mitochondrial; D012333:RNA, Messenger; D012336:RNA, Ribosomal, 16S; D004247:DNA; D009245:NADH Dehydrogenase