Inductions of ornithine decarboxylase and replicative DNA synthesis but not DNA single strand scission or unscheduled DNA synthesis in the pyloric mucosa of rat stomach by catechol.
The possible tumor-promoting and genotoxic activities of catechol were examined. Administration of catechol by gastric intubation at doses of 10 to 90 mg/kg body weight to male F344 rats induced up to 19-fold increase in ornithine decarboxylase activity with a maximum after 8 h and up to 8-fold increase in replicative DNA synthesis with a maximum after 24 h in the pyloric mucosa of the stomach. These results suggest that catechol has tumor-promoting activity in the pyloric mucosa of rat stomach. However, its administration at doses of 37.5 to 90 mg/kg body weight did not induce DNA single strand scission in the pyloric mucosa as determined by the alkaline elution method after 2 and 6 h or unscheduled DNA synthesis examined after 2 and 12 h.
Furihata
C
C
Department of Molecular Oncology, University of Tokyo.
Hatta
A
A
Matsushima
T
T
D000818:Animals; D002396:Catechols / Q000633:toxicity*; D004247:DNA / Q000096:biosynthesis*; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects*; D004261:DNA Replication; D004277:DNA, Single-Stranded / Q000187:drug effects*; D004790:Enzyme Induction / Q000187:drug effects; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009955:Ornithine Decarboxylase / Q000096:biosynthesis*; D011706:Pyloric Antrum / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344
D002396:Catechols; D004277:DNA, Single-Stranded; D004247:DNA; D009955:Ornithine Decarboxylase; C034221:catechol