Minute amounts of RNA are synthesized from several regions of the bacteriophage Mu DNA during the lysogenic state.
The transcription of phage Mu DNA during the lysogenic state has been quantitatively analysed. For this purpose pulse-labelled RNA from two lysogens and from their nonlysogenic parental strains were hybridized to non-overlapping Mu DNA restriction fragments covering the whole phage genome. The data revealed that all regions of the prophage are transcribed at low rates and that phage promoters are involved in this transcription. For this study an improved assay for quantitative filter hybridization was employed. The high sensitivity and reproducibility that can be obtained with the assay make it suitable for the quantitative analysis of minute amounts of mRNA.
Barron
C
C
Fakultät für Biologie, Universität Konstanz, F.R.G.
Bade
E G
EG
D010583:Bacteriophage mu / Q000235:genetics* / Q000378:metabolism; D004279:DNA, Viral / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D007700:Kinetics; D008242:Lysogeny; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012367:RNA, Viral / Q000096:biosynthesis* / Q000235:genetics; D014158:Transcription, Genetic
D004279:DNA, Viral; D012367:RNA, Viral