Development and application of an ELISA technique for the detection of antibody to avian encephalomyelitis viruses.
A rapid sensitive enzyme-linked immunosorbent assay for the detection of antibody to avian encephalomyelitis viruses (AEVs) in chickens using purified antigen is described. The procedure differed from others which have been described for AEV, in that it involved a negative antigen subtraction step which accounted for the variable adhesiveness of chicken sera to plastic surfaces. The procedure was reproducible (between-assay coefficient of variation 8.95 per cent) and a good correlation was observed with results obtained by neutralisation index tests (r = 0.91, P less than 0.1). The assay detects only AEV-specific antibody and allows monitoring of the spread of AEV in flocks.
Shafren
D R
DR
Department of Biological Sciences, University of Newcastle, New South Wales, Australia.
Tannock
G A
GA
Roberts
T K
TK
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002645:Chickens / Q000276:immunology*; D001350:Encephalomyelitis Virus, Avian / Q000276:immunology* / Q000302:isolation & purification; D004770:Enterovirus / Q000276:immunology*; D004769:Enterovirus Infections / Q000276:immunology / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay; D009500:Neutralization Tests; D011201:Poultry Diseases / Q000276:immunology*; D013997:Time Factors
D000914:Antibodies, Viral