Atrial natriuretic peptide vascular receptor: regulation of low-affinity binding by calcium.
1. In the presence of calcium, both high- [dissociation equilibrium constant (Kd) 5.8 x 10(-11) mol/l] and low- (Kd 1.2 x 10(-8) mol/l) affinity binding of atrial natriuretic peptide was detected on plasma membranes prepared from sodium-depleted rats. 2. In contrast, under calcium-free conditions only high-affinity binding (Kd 6.9 x 10(-11) mol/l) was detected. 3. The requirement for calcium for low-affinity binding suggests a different mechanism of action than that for the high-affinity receptor.
Morton
J J
JJ
MRC Blood Pressure Unit, Western Infirmary, Glasgow, U.K.
Beattie
E C
EC
Lyall
F
F
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D017461:Receptors, Atrial Natriuretic Factor; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012964:Sodium / Q000172:deficiency; D012982:Sodium, Dietary / Q000008:administration & dosage
D011956:Receptors, Cell Surface; D012982:Sodium, Dietary; D009320:Atrial Natriuretic Factor; D012964:Sodium; D017461:Receptors, Atrial Natriuretic Factor; D002118:Calcium