Differentiation of hog cholera and bovine virus diarrhoea viruses in pigs using monoclonal antibodies.
Monoclonal antibodies against hog cholera and bovine viral diarrhoea viruses were assayed on organ tissue sections of experimentally infected animals. The animals had been infected simultaneously with both viruses. The antibodies were tested using an indirect immunofluorescence test and an indirect enzyme immunoassay with a biotin/streptavidin/peroxidase detection system. A polyclonal hyperimmune serum was used as a control in direct immunofluorescence tests. Both techniques based on monoclonal antibodies were more sensitive and more specific than the conventional test, the enzyme immunoassay being more sensitive than the immunofluorescence test. Small amounts of BVD viral antigen were demonstrable with monoclonal antibodies in most organ tissues.
Zhou
Y
Y
Moennig
V
V
Coulibaly
C O
CO
Dahle
J
J
Liess
B
B
D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis; D006691:Classical Swine Fever / Q000150:complications / Q000175:diagnosis*; D006692:Classical Swine Fever Virus / Q000276:immunology*; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology*; D010576:Pestivirus / Q000276:immunology*; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*; D014036:Togaviridae Infections / Q000150:complications / Q000175:diagnosis / Q000662:veterinary*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral