Recombinant interferon-gamma is a potent inhibitor of osteoblastic cell functions.
Interleukin-1 and tumor necrosis factor-stimulated bone resorption is mediated by osteoclast-activating factor elaborated by osteoblasts. Recombinant interferon-gamma inhibits stimulation of bone resorption by these cytokines. We examined here the effects of recombinant mouse interferon-gamma (rmIFN-G) on DNA and collagen synthesis, and on alkaline phosphatase (ALP) activity, in the osteoblastic cell line (MC3T3-E1) under confluent culture conditions. Addition of rmIFN-G to the cells markedly inhibited their DNA synthesis and ALP activity in dose- and culture time-related manner. Also we found that rmIFN-G decreased markedly collagen synthesis at day 3 after addition of the agent. These data indicate that rmIFN-G is a potent inhibitor of osteoblastic cell functions.
Hirose
K
K
Hanazawa
S
S
Amano
S
S
Kikuchi
H
H
Takeshita
A
A
Murakami
Y
Y
Hashimoto
H
H
Yokoyama
T
T
Suyama
R
R
Kitano
S
S
D000469:Alkaline Phosphatase / Q000378:metabolism; D001862:Bone Resorption; D002454:Cell Differentiation / Q000187:drug effects*; D003094:Collagen / Q000096:biosynthesis; D007371:Interferon-gamma / Q000494:pharmacology*; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism*; D011994:Recombinant Proteins
D011994:Recombinant Proteins; D007371:Interferon-gamma; D003094:Collagen; D000469:Alkaline Phosphatase