title: Immunoreactive evidence of beta-endorphin and methionine-enkephalin-Arg-Gly-Leu in human tooth pulp.
abstract: For the first time, beta-endorphin-like immunoreactivity (BE-LI) has been measured in human tooth pulp. Separation of peptides from pulp tissue was achieved by acid extraction followed by chromatographic separation through a Sep-Pak disposable cartridge. Reversed phase high performance liquid chromatographic (RP-HPLC) was performed on the peptide-rich fractions for further peptide separation. Radioreceptor assay (RRA) data of the HPLC fractions was used to construct a profile of opioid-receptor active peptides. Radioimmunoassay (RIA) data provided further information. Following acute mechanical stress, a monotonic decrease in BE-LI concentrations was evident according to a four bicuspid extraction order.
authors.affiliation: Department of Orthodontics, College of Dentistry, University of Tennessee, Memphis 38163.
authors.forename: Q C
authors.initials: QC
authors.lastname: Robinson
authors.forename: J T
authors.initials: JT
authors.lastname: Killmar
authors.forename: D M
authors.initials: DM
authors.lastname: Desiderio
authors.forename: E F
authors.initials: EF
authors.lastname: Harris
authors.forename: G
authors.initials: G
authors.lastname: Fridland
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002851:Chromatography, High Pressure Liquid; D003782:Dental Pulp / Q000032:analysis*; D004744:Enkephalin, Methionine / Q000031:analogs & derivatives* / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D009479:Neuropeptides / Q000032:analysis; D011863:Radioimmunoassay; D011869:Radioligand Assay; D001615:beta-Endorphin / Q000032:analysis*
chemical_list: D009479:Neuropeptides; D004744:Enkephalin, Methionine; D001615:beta-Endorphin