Identification of an acidic fibroblast growth factor-like activity in a mesoblastic nephroma.
An acidic fibroblast growth factor-like activity was detected in a primary mesoblastic nephroma. Identification was based upon Northern blot analysis of renal tumor RNA and by a biochemical characterization of the growth factor activity. The activity extracted from the tumor stimulated DNA synthesis in both mouse fibroblasts and bovine endothelial cells. This activity was heat-sensitive and enhanced in the presence of heparin. The extract activity bound to immobilized immobilized heparin, eluting at 1.2 M NaCl, and cross-reacted with antiserum to recombinant acidic fibroblast growth factor. We also present evidence for acidic fibroblast growth factor-like molecules in normal immature human kidney, although the amount of the activity detected is less than that in the mesoblastic nephroma. These data suggest that acidic fibroblast growth factor-like activity is present in the developing normal human kidney, and that some renal tumors can continue to express a similar activity.
Witte
D P
DP
Department of Biochemistry and Molecular Biology, University of Cincinnati College of Medicine, Ohio.
Nagasaki
T
T
Stambrook
P
P
Lieberman
M A
MA
D015152:Blotting, Northern; D002460:Cell Line; D005591:Chemical Fractionation; D004247:DNA / Q000096:biosynthesis; D015342:DNA Probes; D004797:Enzyme-Linked Immunosorbent Assay; D005346:Fibroblast Growth Factors / Q000032:analysis*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007680:Kidney Neoplasms / Q000032:analysis*; D009693:Nucleic Acid Hybridization; D012334:RNA, Neoplasm / Q000032:analysis; D009396:Wilms Tumor / Q000032:analysis*
D015342:DNA Probes; D012334:RNA, Neoplasm; D005346:Fibroblast Growth Factors; D004247:DNA