title: Preparation of high purity IgG and its application in screening for clones positive for Vibrio succinogenes L-asparaginase gene.
abstract: A previous paper reported application of L-asparaginase IgG from Vibrio succinogenes in screening for positive clones. This paper describes the detailed procedure for preparation of high-purity IgG, an essential step of which involves coupling of a cell-free extract of host E. coli Y 1090 to Sepharose 4B beads and adsorption of the non-specific antigen components by passage of the polyvalent IgG through this affinity column. The IgG thus obtained exhibited improved specificity and was utilized as a radioimmunologic and horseradish peroxidase-linked immunologic probe in screening for positive clones. These IgG probes had the advantages of low background, high sensitivity, and good reliability.
authors.affiliation: Shanghai Institute of Plant Physiology, Academia Sinica.
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Guan
authors.forename: H L
authors.initials: HL
authors.lastname: Pong
authors.forename: Y S
authors.initials: YS
authors.lastname: He
authors.forename: J M
authors.initials: JM
authors.lastname: Chen
authors.forename: K Q
authors.initials: KQ
authors.lastname: Ren
mesh_terms: D001215:Asparaginase / Q000032:analysis / Q000235:genetics*; D015153:Blotting, Western; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D015698:Genomic Library; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000276:immunology / Q000302:isolation & purification*; D014733:Vibrio / Q000201:enzymology / Q000235:genetics*
chemical_list: D007074:Immunoglobulin G; D001215:Asparaginase