Inhibition of lymphocyte proliferation by aqueous humor.
Antigens introduced into the anterior chamber (AC) of the eye elicit systemic, antigen-specific suppression of delayed hypersensitivity combined with primed cytotoxic T cells and elevated levels of serum antibodies, a unique immune response termed anterior chamber associated immune deviation (ACAID). Among the mechanisms that have been implicated in the induction of this response is the possibility that T cells first recognize antigen within the AC, where-upon they then escape to initiate the induction of systemic suppression. Because this possibility implies that T-cell recognition of antigen could occur in the aqueous humor (AqH) that normally fills the AC, we tested the effects of freshly obtained AqH on lymphocyte proliferative responses in vitro. The results indicate that AqH from mice and rabbits profoundly inhibits (1) T-lymphocyte proliferation to antigens, (2) T- and B-lymphocyte responses to polyclonal mitogens, and (3) growth-factor-driven lymphocyte proliferation. The antiproliferative activity of AqH was also effective on some, but not all, neoplastic cells. The activity was shown to be neither species specific nor directly cytotoxic to cells. The activity was distinct from the growth inhibitory effects of normal mouse serum. We conclude that AqH contains a soluble factor(s) that is a potent inhibitor of cell proliferation. The potential role(s) of this activity in ocular wound healing and in intraocular immune responses are discussed.
Kaiser
C J
CJ
Department of Immunology and Microbiology, University of Miami School of Medicine, FL 33101.
Ksander
B R
BR
Streilein
J W
JW
D000818:Animals; D000941:Antigens / Q000276:immunology; D001082:Aqueous Humor / Q000032:analysis / Q000276:immunology*; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D006131:Growth Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D007378:Interleukins / Q000037:antagonists & inhibitors; D008213:Lymphocyte Activation / Q000187:drug effects*; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D008934:Mitogens / Q000037:antagonists & inhibitors; D011817:Rabbits; D013045:Species Specificity; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000941:Antigens; D006131:Growth Inhibitors; D007378:Interleukins; D008934:Mitogens