Genetic basis of attenuation of the Sabin type 3 oral poliovirus vaccine.
The poliovirus type 3 Sabin oral poliovirus vaccine strain P3/Leon/12a1b differs in nucleotide sequence from its neurovirulent progenitor P3/Leon/37 by just 10 point mutations. The contribution of each mutation to the attenuation phenotype of the vaccine strain was determined by the construction of a series of recombinant viruses from infectious cDNA clones. The neurovirulence testing of recombinant viruses indicated that the attenuation phenotype is determined by just two point mutations: a C to U in the noncoding region at position 472 and a C to U at nucleotide 2034 which results in a serine-to-phenylalanine amino acid substitution in the structural protein VP3.
Westrop
G D
GD
Department of Microbiology, University of Reading, United Kingdom.
Wareham
K A
KA
Evans
D M
DM
Dunn
G
G
Minor
P D
PD
Magrath
D I
DI
Taffs
F
F
Marsden
S
S
Skinner
M A
MA
Schild
G C
GC
D000595:Amino Acid Sequence; D000818:Animals; D001681:Biological Assay; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004252:DNA Mutational Analysis; D005814:Genes, Viral; D009420:Nervous System / Q000382:microbiology; D017955:Poliovirus / Q000235:genetics* / Q000472:pathogenicity; D011055:Poliovirus Vaccine, Oral / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D013329:Structure-Activity Relationship; D014613:Vaccines, Attenuated / Q000235:genetics*
D011055:Poliovirus Vaccine, Oral; D012367:RNA, Viral; D014613:Vaccines, Attenuated; D004247:DNA