Electrochemical method for measuring C-reactive protein using crown ether--phosphate ester ionophores.
A new electrochemical method for measurement of C-reactive protein (CRP) is presented. We have synthesized new crown ether-phosphate ester ionophores having high affinity for CRP. A study using proton nuclear magnetic resonance shows CRP binding to the ionophores in stoichiometric amounts. The incorporation of these crown ether-phosphate ester ionophores into poly(vinyl chloride) membrane electrodes yields a CRP-sensitive electrode with sensitivity in the microgram per milliliter range. The ionophore synthesis and preliminary electrode characteristics are described.
Merritt
C M
CM
Winkelman
J W
JW
D002097:C-Reactive Protein / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D004952:Esters; D004988:Ethers, Cyclic; D006801:Humans; D007202:Indicators and Reagents; D007476:Ionophores; D011199:Potentiometry
D004952:Esters; D004988:Ethers, Cyclic; D007202:Indicators and Reagents; D007476:Ionophores; D002097:C-Reactive Protein