Neutral endopeptidase 24.11 and angiotensin converting enzyme like activity in CALLA positive and CALLA negative lymphoid cells.
Taking advantage of the recently demonstrated identity of common acute lymphoblastic leukemia antigen (CALLA) and neutral endopeptidase EC.24.11 (NEP) the presence of this ectoenzyme on lymphoid cells has been reassessed using highly sensitive assays (cleavage of [3H]-D-Ala2-leucine-enkephalin and binding of the inhibitor [3H]HACBO-Gly. NEP activity was found not only on already classified CALLA + ve cells but also on numerous cells (including mature B and polyclonal T cells) previously considered as CALLA-ve. This suggests that CALLA/NEP is expressed all along the differentiation pathway in B and T cell lineage. Moreover substantial ACE-like activity was also detected in three tested cells, all of the pre-B phenotype. The availability of specific inhibitors for these enzymes should help clarify their role in cell-differentiation.
Beaumont
A
A
Département de Chimie Organique, U 266 INSERM, UA 498 CNRS, Paris, France.
Brouet
J C
JC
Roques
B P
BP
D000818:Animals; D000943:Antigens, Differentiation / Q000032:analysis*; D000951:Antigens, Neoplasm / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D001402:B-Lymphocytes / Q000201:enzymology; D001921:Brain / Q000201:enzymology; D002462:Cell Membrane / Q000201:enzymology; D004743:Enkephalin, Leucine / Q000031:analogs & derivatives / Q000378:metabolism; D016308:Enkephalin, Leucine-2-Alanine / Q000031:analogs & derivatives*; D005998:Glycine / Q000031:analogs & derivatives / Q000378:metabolism; D006801:Humans; D006898:Hydroxylamines / Q000378:metabolism; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000201:enzymology* / Q000276:immunology; D007945:Leukemia, Lymphoid / Q000201:enzymology* / Q000276:immunology; D008214:Lymphocytes / Q000201:enzymology* / Q000276:immunology; D051379:Mice; D015260:Neprilysin / Q000378:metabolism*; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism*; D013601:T-Lymphocytes / Q000201:enzymology; D014407:Tumor Cells, Cultured
D000943:Antigens, Differentiation; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D006898:Hydroxylamines; D004743:Enkephalin, Leucine; D016308:Enkephalin, Leucine-2-Alanine; C030279:enkephalinamide-Leu, Ala(2)-; C046556:N-(3-hydroxyaminocarbonyl-2-benzyl-1-oxopropyl)glycine; D007703:Peptidyl-Dipeptidase A; D015260:Neprilysin; D005998:Glycine