Isolation and characterization of the complete human beta-myosin heavy chain gene.
The entire gene coding for the human beta-myosin heavy chain has been isolated from genomic EMBL3A phage libraries by chromosomal walking starting from clone gMHC-1, reported earlier (Appelhans and Vosberg 1983). gMHC-1 has been shown to carry coding information for the C-terminal two-thirds of beta-myosin heavy chain, which is expressed in cardiac muscle and in slow skeletal muscle fibers (Lichter et al. 1986). Three DNA clones were identified as overlapping with gMHC-1 by restriction mapping and DNA sequencing. They span a 30-kb region in the genome. About 22 kb extend from the initiation codon ATG to the poly(A) addition site. The clones include about 4 kb of 5' flanking sequences upstream of the promoter. Comparisons of beta- and alpha-myosin heavy chain sequences indicate that gene duplication of the cardiac myosin heavy chain isogenes preceded the mammalian species differentiation.
Diederich
K W
KW
Max-Planck-Institut für medizinische Forschung, Abteilung für Molekulare Biologie, Heidelberg, Federal Republic of Germany.
Eisele
I
I
Ried
T
T
Jaenicke
T
T
Lichter
P
P
Vosberg
H P
HP
D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D006801:Humans; D008969:Molecular Sequence Data; D009218:Myosins / Q000235:genetics*; D011817:Rabbits; D015183:Restriction Mapping
D009218:Myosins