Regulation of phagolysosome pH in bovine and human neutrophils: the role of NADPH oxidase activity and an Na+/H+ antiporter.
Fluorescein-labelled Staphylococcus aureus were used to follow changes in phagolysosome (PL) pH of bovine and human neutrophils following phagocytosis. Under aerobic conditions there was an alkalinisation of the PL followed by a slow decline. Under anaerobic conditions no alkalinisation of the PL was seen, and pharmacological inhibition of the NADPH oxidase with diphenyleneiodonium (DPI) resulted in a rapid acidification of the PL following phagocytosis. The inclusion of amiloride, an inhibitor of Na+/H+ antiporter activity, produced a more rapid alkalinisation phase following phagocytosis under aerobic conditions and reduced, but did not abolish, the acidification phases seen under anaerobic conditions or following treatment of neutrophils with DPI. The results suggest that PL pH is influenced by NADPH oxidase activity and to a lesser extent by a Na+/H+ antiporter. The antibacterial effectiveness of neutrophil granule proteins may be affected under conditions that influence the functioning of these two systems.
Mayer
S J
SJ
Department of Veterinary Medicine, University of Bristol Veterinary School, Langford, England.
Keen
P M
PM
Craven
N
N
Bourne
F J
FJ
D000584:Amiloride / Q000494:pharmacology; D000693:Anaerobiosis; D002352:Carrier Proteins / Q000502:physiology*; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007231:Infant, Newborn; D008247:Lysosomes / Q000378:metabolism*; D009247:NADH, NADPH Oxidoreductases / Q000502:physiology*; D019255:NADPH Oxidases; D009504:Neutrophils / Q000378:metabolism*; D009861:Onium Compounds / Q000494:pharmacology; D010587:Phagocytosis; D017923:Sodium-Hydrogen Exchangers
D002352:Carrier Proteins; D009861:Onium Compounds; D017923:Sodium-Hydrogen Exchangers; C007517:diphenyleneiodonium; D000584:Amiloride; D009247:NADH, NADPH Oxidoreductases; D019255:NADPH Oxidases