Nisin inhibits several gram-positive, mastitis-causing pathogens.
Organisms known to cause bovine mastitis, Enterococcus faecalis ssp. liquefaciens ATCC 27959, Staphylococcus aureus ATCC 29740, Streptococcus agalactiae ATCC 27956, Streptococcus equinus ATCC 27960, Streptococcus dysgalactiae ATCC 27957, Streptococcus uberis ATCC 27958, and the neotype Staphylococcus epidermidis ATCC 14990 were examined for their susceptibility to the small peptide antibiotic, nisin. Using a disc assay, minimum inhibitory concentrations of nisin ranged from 10 to 250 micrograms/ml among the strains. Examination of the antimicrobial effect of 50 micrograms/ml nisin in milk showed nisin inhibited all gram-positive pathogens tested.
Broadbent
J R
JR
Department of Nutrition and Foods Sciences, Utah State University, Logan 84322-8700.
Chou
Y C
YC
Gillies
K
K
Kondo
J K
JK
D000818:Animals; D002417:Cattle; D015169:Colony Count, Microbial; D013293:Enterococcus faecalis / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects*; D008414:Mastitis, Bovine / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D008892:Milk / Q000382:microbiology; D009561:Nisin / Q000494:pharmacology*; D013210:Staphylococcus / Q000187:drug effects*; D013211:Staphylococcus aureus / Q000187:drug effects; D013212:Staphylococcus epidermidis / Q000187:drug effects; D013291:Streptococcus / Q000187:drug effects*; D013292:Streptococcus agalactiae / Q000187:drug effects
D009561:Nisin