Point mutational inactivation of the retinoblastoma antioncogene.
The retinoblastoma (Rb) antioncogene encodes a nuclear phosphoprotein, p105-Rb, that forms protein complexes with the adenovirus E1A and SV40 large T oncoproteins. A novel, aberrant Rb protein detected in J82 bladder carcinoma cells was not able to form a complex with E1A and was less stable than p105-Rb. By means of a rapid method for the detection of mutations in Rb mRNA, this defective Rb protein was observed to result from a single point mutation within a splice acceptor sequence in J82 genomic DNA. This mutation eliminates a single exon and 35 amino acids from its encoded protein product.
Horowitz
J M
JM
Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge 02142.
Yandell
D W
DW
Park
S H
SH
Canning
S
S
Whyte
P
P
Buchkovich
K
K
Harlow
E
E
Weinberg
R A
RA
Dryja
T P
TP
D017456:Adenovirus Early Proteins; D000952:Antigens, Polyomavirus Transforming; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000378:metabolism; D005134:Eye Neoplasms / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D009856:Oncogene Proteins, Viral / Q000378:metabolism; D009857:Oncogenes; D010750:Phosphoproteins / Q000235:genetics* / Q000378:metabolism; D012175:Retinoblastoma / Q000235:genetics*; D016160:Retinoblastoma Protein
D017456:Adenovirus Early Proteins; D000952:Antigens, Polyomavirus Transforming; D004268:DNA-Binding Proteins; D009856:Oncogene Proteins, Viral; D010750:Phosphoproteins; D016160:Retinoblastoma Protein