[Expression of O-glycans during enterocytic differentiation of HT-29 cells].
The expression of O- and N-glycan chains during the enterocytic differentiation of HT-29 cells was followed using L-[63H]-fucose and D-[6-3H]-glucosamine radiolabeling. Whatever the cell population, i.e., differentiated or undifferentiated, the incorporation of radiolabeled sugars into glycoproteins was similar. However, differences among these two cell populations were noted when the ratio [3H]-glucosamine/[3H]-galactosamine and the sensitivity of glycopeptides to mild alkaline treatment were followed. From these data, we could conclude that there is a shift in the high molecular weight glycopeptides during the differentiation of HT-29 cells meaning an increase of O-linked glycopeptides correlated with a decrease of N-linked forms.
Ogier-Denis
E
E
Unité de Recherche sur la Biologie et la Pathologie moléculaires des Glycoprotéines, I.N.S.E.R.M. U. 180, Paris.
Trugnan
G
G
Tran
A T
AT
Sapin
C
C
Aubery
M
M
Codogno
P
P
D000230:Adenocarcinoma / Q000473:pathology; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology; D005944:Glucosamine / Q000378:metabolism; D006801:Humans; D008970:Molecular Weight; D011134:Polysaccharides / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000473:pathology
D011134:Polysaccharides; D005944:Glucosamine
Expression des O-glycannes au cours de la différenciation entérocytaire des cellules HT-29.