Activated peripheral blood mononuclear cells a possible substitute to B-lymphocytes in HLA-DR determination.
The serological determination of class II antigens is still a mandatory test prior to allotransplantation. It is known that these antigens are normally expressed on B-lymphocytes and monocytes. The B-lymphocytes that constitute 10-15% of total blood lymphocytes are the cells currently used for HLA-DR typing. To avoid HLA-DR typing difficulties, or even impossibilities that are frequently encountered among some patient groups, we studied the response of peripheral blood mononuclear cells (PBMNC)--as an alternative source of cells for class II antigen typing--to in vitro mitogen and interleukin-2 activation and propagation. Although the patients included in this study were selected having pre-known HLA-DR typing difficulties, all of them could be adequately typed by this method.
el-Barrawy
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MA
D001402:B-Lymphocytes; D006684:HLA-DR Antigens / Q000302:isolation & purification*; D006650:Histocompatibility Testing; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008213:Lymphocyte Activation
D006684:HLA-DR Antigens