Effect of polyamines on mitochondrial F1-ATPase catalyzed reactions.
The effect of polyamines on F1-ATPase catalyzed reactions has been studied through the use of submitochondrial particles and F1-ATPase. ATP degradation catalyzed by submitochondrial particles and F1-ATPase was inhibited by spermine and spermidine. Spermine's inhibition was much greater than spermidine's effect. In contrast, P1-ATP exchange and succinate dependent ATP synthesis catalyzed by submitochondrial particles were both stimulated by spermine. The inhibition of ATPase activity by polyamines probably occurs through polyamine's replacement of Mg2+ on ATP, for the following reasons. (a) The ATPase activity inhibited by spermine was partially recovered when Mg2+ was added. (b) Spermine bound to ATP and phospholipids but not to F1-ATPase; yet spermine inhibited the ATPase reaction catalyzed by F1-ATPase, a protein free of phospholipid. (c) The binding of spermine to ATP was inhibited by Mg2+. The ATP content in polyamine-deficient cells definitely was lower than that in normal cells. On the basis of these results, the possible role of spermine in keeping the ATP concentration at a high level is discussed.
Igarashi
K
K
Faculty of Pharmaceutical Sciences, Chiba University.
Kashiwagi
K
K
Kobayashi
H
H
Ohnishi
R
R
Kakegawa
T
T
Nagasu
A
A
Hirose
S
S
D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D015317:Biogenic Polyamines / Q000378:metabolism / Q000494:pharmacology*; D002478:Cells, Cultured; D066298:In Vitro Techniques; D007700:Kinetics; D008930:Mitochondria, Liver / Q000187:drug effects / Q000201:enzymology*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D051381:Rats; D013096:Spermine / Q000378:metabolism; D013367:Submitochondrial Particles / Q000378:metabolism
D015317:Biogenic Polyamines; D010743:Phospholipids; D010761:Phosphorus Radioisotopes; D013096:Spermine; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D009247:NADH, NADPH Oxidoreductases; D006180:Proton-Translocating ATPases