title: Porcine brain natriuretic peptide receptor in bovine adrenal cortex.
abstract: The action of porcine brain natriuretic peptide (pBNP) on the steroidogenesis was investigated in cultured bovine adrenocortical cells. Porcine BNP induced a significant dose-dependent inhibition of both ACTH- and A II-stimulated aldosterone secretion. 10(-8) M and 10(-7) M pBNP also significantly inhibited ACTH-stimulated cortisol and dehydroepiandrosterone (DHEA) secretions. Binding studies of [125I]-pBNP to bovine adrenocortical membrane fractions showed that adrenal cortex had high-affinity and low-capacity pBNP binding sites, with a dissociation constant (Kd) of 1.70 x 10(-10) M and a maximal binding capacity (Bmax) of 19.9 fmol/mg protein. Finally, the 135 Kd radioactive band was specially visualized in the affinity labeling of bovine adrenal cortex with disuccinimidyl suberate (DSS). These results suggest that pBNP may have receptor-mediated suppressive actions on bovine adrenal steroidogenesis, similar to that in atrial natriuretic peptide (ANP).
authors.affiliation: Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Higuchi
authors.forename: T
authors.initials: T
authors.lastname: Hashiguchi
authors.forename: M
authors.initials: M
authors.lastname: Ohashi
authors.forename: R
authors.initials: R
authors.lastname: Takayanagi
authors.forename: M
authors.initials: M
authors.lastname: Haji
authors.forename: H
authors.initials: H
authors.lastname: Matsuo
authors.forename: H
authors.initials: H
authors.lastname: Nawata
mesh_terms: D000302:Adrenal Cortex / Q000187:drug effects / Q000378:metabolism*; D000345:Affinity Labels; D000450:Aldosterone / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003687:Dehydroepiandrosterone / Q000378:metabolism; D006854:Hydrocortisone / Q000378:metabolism; D008970:Molecular Weight; D020097:Natriuretic Peptide, Brain; D009419:Nerve Tissue Proteins / Q000378:metabolism* / Q000494:pharmacology; D011869:Radioligand Assay; D017461:Receptors, Atrial Natriuretic Factor; D011956:Receptors, Cell Surface / Q000378:metabolism*
chemical_list: D000345:Affinity Labels; D009419:Nerve Tissue Proteins; D011956:Receptors, Cell Surface; C058930:brain natriuretic peptide receptor; D020097:Natriuretic Peptide, Brain; D003687:Dehydroepiandrosterone; D000450:Aldosterone; D017461:Receptors, Atrial Natriuretic Factor; D006854:Hydrocortisone