title: A DNA probe from Schistosoma mansoni allows rapid determination of the sex of larval parasites.
abstract: A DNA clone representing a 0.4 kb degenerative repeat has been isolated. The DNA sequence is present only in the genome of female Schistosoma mansoni at different stages of the life cycle, at a frequency of approximately 75 copies per adult female genome. The sequence is not expressed and probably represents satellite DNA in the heterochromatin region of the W chromosome. It is demonstrated that the DNA clone may be used for the rapid determination of the sex of cercariae without the need for DNA isolation or Southern blotting.
authors.affiliation: Department of Zoology, British Museum (Natural History), London, U.K.
authors.forename: T K
authors.initials: TK
authors.lastname: Walker
authors.forename: D
authors.initials: D
authors.lastname: Rollinson
authors.forename: A J
authors.initials: AJ
authors.lastname: Simpson
mesh_terms: D000818:Animals; D001345:Autoradiography; D010582:Bacteriophage lambda / Q000235:genetics; D015139:Blotting, Southern; D015342:DNA Probes; D005260:Female; D007814:Larva / Q000235:genetics; D008297:Male; D012091:Repetitive Sequences, Nucleic Acid; D012550:Schistosoma mansoni / Q000235:genetics*; D012728:Sex Chromatin / Q000032:analysis*; D012737:Sex Factors; D013045:Species Specificity
chemical_list: D015342:DNA Probes