Structure of the Marek's disease virus BamHI-H gene family: genes of putative importance for tumor induction.
The BamHI H fragment of Marek's disease virus (MDV), which has been hypothesized to contain a tumor-inducing gene, was partially sequenced and analyzed for transcriptional activity. Transcription of several RNAs was found to initiate within BamHI-H, with the major transcripts initiating on either side of a putative MDV origin of lytic replication. A 1.8-kilobase gene family found to be produced only by pathogenic MDV was detected, and its transcription map was produced. It is hypothesized that the 1.8-kilobase gene family is directly associated with the tumorigenic potential of MDV.
Bradley
G
G
Department of Medical Microbiology, School of Medicine, University of South Florida, Tampa 33612.
Hayashi
M
M
Lancz
G
G
Tanaka
A
A
Nonoyama
M
M
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002478:Cells, Cultured; D002642:Chick Embryo; D004279:DNA, Viral / Q000235:genetics*; D015245:Deoxyribonuclease BamHI; D004720:Endonucleases; D005091:Exons; D005347:Fibroblasts; D005784:Gene Amplification; D005814:Genes, Viral; D008381:Herpesvirus 2, Gallid / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000235:genetics; D015183:Restriction Mapping; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013047:Specific Pathogen-Free Organisms; D014158:Transcription, Genetic
D004279:DNA, Viral; D012367:RNA, Viral; D004720:Endonucleases; D015245:Deoxyribonuclease BamHI; D015719:Single-Strand Specific DNA and RNA Endonucleases