title: Effect of mutations causing gluconate kinase or gluconate permease deficiency on expression of the Bacillus subtilis gnt operon.
abstract: The gluconate (gnt) operon contains genes for a repressor of the operon, gluconate kinase, and gluconate permease. A nonleaky kinase mutation (gntK4) induced the gnt operon constitutively through interaction of the repressor with an inducer of gluconate which had been endogenously formed and accumulated in the cell owing to the complete deficiency of the kinase even in the absence of gluconate in the medium. In contrast, a nonleaky permease mutation (gntP9) never induced the operon by gluconate likely because it cannot give rise to its inducing concentration in the cell even in the presence of gluconate in the medium.
authors.affiliation: Department of Biotechnology, Faculty of Engineering, Fukuyama University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Fujita
authors.forename: T
authors.initials: T
authors.lastname: Fujita
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics*; D001435:Bacteriophages / Q000235:genetics; D001483:Base Sequence; D029968:Escherichia coli Proteins; D005796:Genes; D005798:Genes, Bacterial; D026901:Membrane Transport Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D009876:Operon; D010770:Phosphotransferases / Q000235:genetics*; D017853:Phosphotransferases (Alcohol Group Acceptor); D015183:Restriction Mapping; D014158:Transcription, Genetic
chemical_list: D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; C104633:gluconate permease, E coli; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C020578:gluconokinase