Distribution of leukosialin (W3/13)-like immunoreactivity in the rat central nervous system.
The monoclonal antibody W3/13, which is directed against leukosialin (a molecule present on certain types of leukocytes), was found to react immunohistochemically with distinct regions and structures in the rat CNS. The leukosialin (W3/13)-like immunoreactivity occurred on neurite-like fibres and on bouton-like structures on nerve cell bodies and their dendrites. This labelling was most pronounced in certain subcortical areas and brain stem nuclei including different thalamic nuclei, olfactory tubercle, superior olive, cochlear and vestibular nuclei, motor and somatosensory nuclei. In the cerebellum, immunoreactive fibres were prominent in the molecular layer. In the spinal cord there was a dense labelling of fibres in the whole grey matter.
Losy
J
J
Department of Pathology, Karolinska Institutet, Huddinge Hospital, Sweden.
Maehlen
J
J
Olsson
T
T
Kristensson
K
K
D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D051917:Leukosialin; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012795:Sialoglycoproteins / Q000378:metabolism*; D013116:Spinal Cord / Q000032:analysis* / Q000166:cytology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D051917:Leukosialin; D012795:Sialoglycoproteins; C497864:Spn protein, rat