Biological activities of interleukin-2 and gamma-interferon in bovine T lymphocyte conditioned media.
Interleukin 2 and gamma-interferon were revealed in cultures of Concanavalin A-activated bovine mononuclear cells from peripheral blood leukocytes and their kinetics of production were described. Cyclosporin A could dramatically inhibit the synthesis of interleukin 2, whereas it did not affect the interleukin 2-dependent proliferation of bovine T blasts. Bovine T lymphocyte conditioned media (TLCM) containing gamma-interferon increased the expression of beta-2 microglobulin in human HEL (Human Embryo Lung) 299 cells and exerted a potent anti-proliferative effect upon bovine Aubek cells. The beta-2 microglobulin modulating activity of bovine gamma-interferon was exerted at very low concentrations, which showed no detectable antiviral activity on human cells.
Amadori
M
M
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia, Brescia, Italy.
Bonizzi
L
L
D000818:Animals; D000970:Antineoplastic Agents; D000998:Antiviral Agents; D002417:Cattle; D002478:Cells, Cultured; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis / Q000494:pharmacology*; D007376:Interleukin-2 / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008213:Lymphocyte Activation; D001613:beta 2-Microglobulin / Q000096:biosynthesis*
D000970:Antineoplastic Agents; D000998:Antiviral Agents; D007376:Interleukin-2; D001613:beta 2-Microglobulin; D007371:Interferon-gamma