A new procedure for the analysis and purification of naturally occurring anatoxin-a from the blue-green alga Anabaena flos-aquae.
Anabaena flos-aquae produces at least two neurotoxins termed anatoxin-a (ANTX-a) and -a(s) [ANTX-a(s)]. ANTX-a is a potent postsynaptic depolarizing neuromuscular blocking agent. Its structure is the secondary amine, 2-acetyl-9-azabicyclo[4.2.1]non-2-ene. Known isolation and analysis methods have not always given satisfactory results. Therefore, an improved procedure was developed for both isolation and analysis of ANTX-a which involves four steps; extraction, clean-up, separation and determination. Extraction was efficiently done with 0.05 M acetic acid, and a reversed phase. ODS (octadecylsilanized) and a cation exchanger (COOH) organosilans bonded to silica gels were applied to a clean-up step. Separation and determination were then performed using two TLC and HPLC chromatographies.
Harada
K
K
Faculty of Pharmacy, Meijo University, Nagoya, Japan.
Kimura
Y
Y
Ogawa
K
K
Suzuki
M
M
Dahlem
A M
AM
Beasley
V R
VR
Carmichael
W W
WW
D001427:Bacterial Toxins; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D000458:Cyanobacteria / Q000032:analysis*; D000087522:Cyanobacteria Toxins; D008387:Marine Toxins / Q000032:analysis / Q000302:isolation & purification*; D013058:Mass Spectrometry; D052998:Microcystins; D014326:Tropanes
D001427:Bacterial Toxins; D000087522:Cyanobacteria Toxins; D008387:Marine Toxins; D052998:Microcystins; D014326:Tropanes; C509783:anatoxin a