Radioimmunoassay for brain natriuretic peptide (BNP) detection of BNP in canine brain.
We established a highly sensitive and specific radioimmunoassay (RIA) for BNP. Our RIA detected BNP-like immunoreactivity (-LI) in the porcine and canine brains but did not detect BNP-LI in the human, monkey or rat brain. The widespread distribution of BNP-LI was demonstrated both in the porcine and canine brains, with the highest concentration in the medulla oblongata. In contrast, the highest concentration of ANP-LI determined simultaneously was in the midbrain and the olfactory bulb. High performance-gel permeation chromatography coupled with RIA revealed that the major component of BNP-LI was eluted at the position of synthetic BNP with a small molecular weight (3K). These results indicate that the RIA for BNP serves as a useful tool to investigate physiological roles of BNP.
Itoh
H
H
Department of Medicine, Kyoto University School of Medicine, Japan.
Nakao
K
K
Saito
Y
Y
Yamada
T
T
Shirakami
G
G
Mukoyama
M
M
Arai
H
H
Hosoda
K
K
Suga
S
S
Minamino
N
N
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000032:analysis*; D001923:Brain Chemistry; D004285:Dogs; D000882:Haplorhini; D006801:Humans; D009928:Organ Specificity; D010902:Pituitary Gland / Q000032:analysis; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D013045:Species Specificity; D013552:Swine
D009320:Atrial Natriuretic Factor