title: Mouse Mos protooncogene product is present and functions during oogenesis.
abstract: We have identified the mouse Mos-encoded protein product, p39mos, in maturing mouse oocytes and have shown that it is indistinguishable from the product expressed in Mos-transformed NIH 3T3 cells. p39mos is detected in oocytes arrested in the first meiotic prophase, during germinal-vesicle breakdown, metaphase I, anaphase I, and in ovulated eggs. We show that microinjection of three different Mos antisense (but not sense) oligodeoxyribonucleotides into germinal vesicle-stage oocytes prevents first polar-body emission and therefore interrupted the normal progression of meiosis. These results show that in mouse oocytes, as in the amphibian Xenopus [Sagata, N., Oskarsson, M., Copeland, T., Brumbaugh, J. & Vande Woude, G.F. (1988) Nature (London) 335, 519-525], the product of Mos is necessary for normal meiotic maturation.
authors.affiliation: BRI-Basic Research Program, National Cancer Institute-Frederick Cancer Research Facility, MD 21701.
authors.forename: R S
authors.initials: RS
authors.lastname: Paules
authors.forename: R
authors.initials: R
authors.lastname: Buccione
authors.forename: R C
authors.initials: RC
authors.lastname: Moschel
authors.forename: G F
authors.initials: GF
authors.lastname: Vande Woude
authors.forename: J J
authors.initials: JJ
authors.lastname: Eppig
mesh_terms: D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D005260:Female; D008540:Meiosis; D051379:Mice; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D009865:Oocytes / Q000166:cytology / Q000502:physiology*; D009866:Oogenesis; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000502:physiology*; D017231:Proto-Oncogene Proteins c-mos; D011519:Proto-Oncogenes; D014981:Xenopus
chemical_list: D015345:Oligonucleotide Probes; D011518:Proto-Oncogene Proteins; D017231:Proto-Oncogene Proteins c-mos