Selective inhibition of interleukin 2 but not of interferon-gamma, tumour necrosis factor-alpha or granulocyte/macrophage colony stimulating factor secretion by human helper T cell clones after antigen-driven tolerisation.
Alloreactive human T-helper cell clones chronically exposed to specific antigen can become tolerised, i.e. lose their autocrine proliferative capacity and no longer undergo antigen-driven clonal expansion. To study parameters of possible clonal anergy in such cells, lymphokine secretion before and after tolerisation was measured. 10/10 CD4+ clones were found to secrete interleukin 2 (IL-2), interferon gamma, tumour necrosis factor alpha and granulocyte/macrophages colony stimulating factors. Tolerised clones which had lost autocrine proliferative capacity and had lost the ability to secret IL-2 nevertheless retained the ability to secrete the other lymphokines. These results thus show a highly selective lesion in the noncoordinate regulation of lymphokine secretion by tolerised cells.
Pawelec
G
G
Immunology Laboratory, Medical Clinic, University of Tübingen, F.R.G.
Sayers
T
T
Busch
F W
FW
D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000378:metabolism*; D003429:Cross Reactions; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000378:metabolism*; D006801:Humans; D007108:Immune Tolerance; D007371:Interferon-gamma / Q000378:metabolism*; D007376:Interleukin-2 / Q000378:metabolism*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D007376:Interleukin-2; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor