The use of a nitrocellulose-enzyme immunoassay for the rapid screening of monoclonal antibodies to human enteroviruses.
A simple, indirect enzyme immunoassay using purified enterovirus adsorbed on to nitrocellulose has been developed for screening monoclonal antibodies to enteroviruses. The sensitivity of the assay ranged from 10 ng to 1 microgram of viral protein and was 10- to 50-fold more sensitive than conventional EIA on microplates. This simple, sensitive and specific assay proved to be a useful and practical tool for detecting monoclonal antibodies which would not be found in a conventional EIA screening procedure.
Fuchs-Beraud
F
F
Laboratoire National de la Santé, Départment d'Etudes des Maladies Virales, Lyon, France.
Aymard
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D000914:Antibodies, Viral / Q000302:isolation & purification*; D003101:Collodion; D003429:Cross Reactions; D004770:Enterovirus / Q000276:immunology*; D029822:Enterovirus B, Human / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D017955:Poliovirus / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D003101:Collodion