[The primary study on purification, characterization and function of rabbit serum HDL].
Rabbit serum HDL was isolated by gradient ultracentrifugation, found to be pure on PAGE, and characterized by PAGIF. The effect of HDL on normal and hyperlipidemic uptake of LDL into SMC through LDL receptors in rabbits was observed in three experiments. The percentage decrease between normal groups with and without HDL was small (2.3%, 5.5%, 7.3%), while the percentage decrease between hyperlipidemic groups with and without was large (23%, 25%, 26%). The results demonstrate that HDL affects the uptake of hyperlipidemic LDL into SMC through LDL receptors. This suggests that HDL might regulate the cholesterol overloading in cells in cases of hyperlipidemic LDL. The mechanism of HDL action is discussed.
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D000818:Animals; D001011:Aorta; D006949:Hyperlipidemias / Q000097:blood*; D008075:Lipoproteins, HDL / Q000302:isolation & purification / Q000494:pharmacology / Q000502:physiology*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000378:metabolism; D011817:Rabbits; D011973:Receptors, LDL / Q000502:physiology
D008075:Lipoproteins, HDL; D011973:Receptors, LDL