Retinoic acid induces transforming growth factor-beta 2 in cultured keratinocytes and mouse epidermis.
We have studied the functional interaction between retinoic acid and transforming growth factor-beta (TGF-beta), using the mouse epidermis as a model system. Treatment with retinoic acid increases expression of TGF-beta 2 in cultured keratinocytes in vitro, as well as in the epidermis in vivo. This TGF-beta 2 is secreted in a biologically active form that can bind to surface receptors, in contrast to most other conditions in which TGF-beta is secreted in a latent form. Specific antibodies to TGF-beta 2 partially reverse the ability of retinoic acid to inhibit DNA synthesis in cultured keratinocytes. The regulation of TGF-beta 2 expression by retinoic acid may have important physiological and pharmacological roles in the maintenance of epidermal homeostasis.
Glick
A B
AB
Laboratory of Chemoprevention, National Cancer Institute, Bethesda, Maryland 20892.
Flanders
K C
KC
Danielpour
D
D
Yuspa
S H
SH
Sporn
M B
MB
D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D005260:Female; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D012867:Skin / Q000187:drug effects* / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology*
D016212:Transforming Growth Factor beta; D014212:Tretinoin; D004247:DNA