title: High-resolution 31P nuclear magnetic resonance study of Chlamydia trachomatis: induction of ATPase activity in elementary bodies.
abstract: ATPase activity of elementary bodies (EBs) of Chlamydia trachomatis was investigated by using high-resolution 31P nuclear magnetic resonance spectroscopy. ATPase activity was detected in EBs of C. trachomatis serovars A, B, and L2 after treatment with the reducing agents 2-mercaptoethanol and glutathione. ATPase activity was oligomycin sensitive and magnesium ion dependent. EBs heated at 60 degrees C for 10 min or pretreated with Triton X-100 before exposure to 2-mercaptoethanol did not exhibit ATPase activity. Monoclonal antibody to the major outer membrane protein abrogated ATPase activity of EBs, whereas monoclonal antibody to chlamydial lipopolysaccharide only marginally reduced the level of ATPase activity. These findings suggest that EBs possess intrinsic ATPase activity and that cysteine-rich outer membrane proteins of EBs are important in the regulation of ATPase activity. The major outer membrane protein may be the major route through which ATP accesses ATPase.
authors.affiliation: Department of Medical Microbiology, University of Manitoba, Winnipeg, Canada.
authors.forename: R W
authors.initials: RW
authors.lastname: Peeling
authors.forename: J
authors.initials: J
authors.lastname: Peeling
authors.forename: R C
authors.initials: RC
authors.lastname: Brunham
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000378:metabolism; D001692:Biological Transport; D002692:Chlamydia trachomatis / Q000201:enzymology* / Q000648:ultrastructure; D004734:Energy Metabolism; D005978:Glutathione / Q000494:pharmacology; D008274:Magnesium / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D008623:Mercaptoethanol / Q000494:pharmacology; D008854:Microscopy, Electron; D009840:Oligomycins / Q000494:pharmacology
chemical_list: D000911:Antibodies, Monoclonal; D009840:Oligomycins; D008623:Mercaptoethanol; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; D005978:Glutathione; D008274:Magnesium