title: Rat atrial natriuretic factor (ANP-III) inhibits phosphate transport in brush border membrane from superficial and juxtamedullary cortex.
abstract: Previous studies have shown that administration of synthetic atrial natriuretic factor (ANF, 101-126) decreases sodium-dependent phosphate transport across renal brush border membrane vesicles (BBMV) in rats fed a normal or low phosphate diet. In the present study, infusion of rat ANF (atriopeptin III (ANP-III), 103-126 rat ANF) to rats fed a normal phosphate diet caused natriuretic and phosphaturic effects similar to those of ANF (101-126), but unlike ANF (101-126) did not increase the glomerular filtration rate. The effect of ANP-III infusion on sodium-dependent transport of phosphate was also determined in BBM vesicles isolated from the superficial cortex (BBMV-SC) and juxtamedullary cortex (BBMV-JM). The results indicate that ANP-III decreases phosphate transport across BBMV-SC and BBMV-JM similarly (20-24%). However, it had no effect on sodium-dependent transport of proline in these vesicles. The infusion of ANP-III to rats fed a normal phosphate diet inhibits phosphate uptake both in BBMV-SC and BBMV-JM and causes phosphaturia without increments in glomerular filtration rate.
authors.affiliation: Department of Physiology and Biophysics, Mayo Clinic and Foundation, Rochester, Minnesota 55905.
authors.forename: A N
authors.initials: AN
authors.lastname: Yusufi
authors.forename: T J
authors.initials: TJ
authors.lastname: Berndt
authors.forename: H
authors.initials: H
authors.lastname: Moltaji
authors.forename: V
authors.initials: V
authors.lastname: Donovan
authors.forename: T P
authors.initials: TP
authors.lastname: Dousa
authors.forename: F G
authors.initials: FG
authors.lastname: Knox
mesh_terms: D000818:Animals; D009320:Atrial Natriuretic Factor / Q000502:physiology*; D001692:Biological Transport; D001794:Blood Pressure; D005919:Glomerular Filtration Rate; D007606:Juxtaglomerular Apparatus / Q000378:metabolism*; D007672:Kidney Cortex / Q000378:metabolism*; D008297:Male; D008871:Microvilli / Q000378:metabolism*; D010280:Parathyroid Glands / Q000502:physiology; D010710:Phosphates / Q000378:metabolism* / Q000652:urine; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D012964:Sodium / Q000652:urine; D013965:Thyroidectomy
chemical_list: D010710:Phosphates; C472610:atrial natriuretic peptide, rat; D009320:Atrial Natriuretic Factor; D012964:Sodium