Cyclic-AMP-induced c-fos expression and its relevance to differentiation of a transformed mast cell line.
The effects of cyclic AMP on expression of the oncogenes c-myc, c-myb and c-fos in murine P815 mastocytoma cells were examined in relation to growth and differentiation. Induction of differentiation in mastocytoma cells by cyclic AMP was accompanied by a rapid increase in c-fos expression. Cyclic AMP induced stable expression of c-fos mRNA by increasing c-fos transcription 4-5-fold and slightly increasing the stability of c-fos mRNA. However, a high level of c-fos expression was not essential for differentiation of two temperature sensitive-mutant P815 cell lines, as c-fos mRNA did not increase in differentiating temperature-sensitive P815 cells. These results do not support an essential role for c-fos expression in the differentiation of mast cells. Although c-myc expression was lower after growth arrest by cyclic AMP, this decrease did not correlate with growth inhibition by cyclic AMP, since c-myc expression decreased only after cells had started to arrest in G1 phase.
Goulding
M D
MD
Department of Cellular and Molecular Biology, University of Auckland, New Zealand.
Ralph
R K
RK
D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D002454:Cell Differentiation; D002455:Cell Division; D002458:Cell Fractionation; D002461:Cell Line, Transformed; D002467:Cell Nucleus / Q000378:metabolism; D000242:Cyclic AMP / Q000502:physiology*; D015342:DNA Probes; D004587:Electrophoresis, Agar Gel; D005786:Gene Expression Regulation; D007399:Interphase; D008407:Mast Cells / Q000166:cytology*; D009857:Oncogenes; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism
D015342:DNA Probes; D012333:RNA, Messenger; D000242:Cyclic AMP