title: Ability of p53 and the adenovirus E1b 58-kilodalton protein to form a complex is determined by p53.
abstract: We have investigated p53-E1b 58-kilodalton (kDa) protein complex formation during permissive and semipermissive infections with adenovirus type 5 (Ad5) dl309. While metabolic labeling studies easily detected p53-E1b 58-kDa protein complexes in transformed rat cells (XhoI-C), the same methods have not revealed complexes during infection of either human osteosarcoma cells (permissive) or normal rat kidney cells (semipermissive). Complexes were not detectable at any stage during the replicative cycle of Ad5 dl309 in osteosarcoma cells, and they could not be stabilized by using an in vivo cross-linking agent. In addition, using the E4-defective mutant Ad5 dl355, no complexes were observed either. Thus, the lack of p53-E1b 58-kDa protein complex formation during infection is not due to competition from the E4 34-kDa protein. In vitro association experiments showed that in vitro-translated mouse and human p53 could form complexes with E1b 58-kDa antigen expressed during infection. Thus, such E1b proteins are competent to form complexes. The converse experiment, in which in vitro-translated E1b 58-kDa protein was mixed with lysates of osteosarcoma cells, showed little or no p53-E1b 58-kDa protein association, even though the in vitro E1b 58-kDa protein could associate stably with p53 from cells containing endogenous p53-E1b 58-kDa protein complex. These data suggest that competence to form p53-E1b 58-kDa protein complexes resides in some property of p53.
authors.affiliation: Marie Curie Research Institute, Oxted, Surrey, England.
authors.forename: A W
authors.initials: AW
authors.lastname: Braithwaite
authors.forename: J R
authors.initials: JR
authors.lastname: Jenkins
mesh_terms: D017456:Adenovirus Early Proteins; D000260:Adenoviruses, Human / Q000235:genetics*; D000818:Animals; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D006801:Humans; D046911:Macromolecular Substances; D051379:Mice; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000502:physiology*; D009856:Oncogene Proteins, Viral / Q000502:physiology*; D010750:Phosphoproteins / Q000502:physiology*; D051381:Rats; D013329:Structure-Activity Relationship; D016159:Tumor Suppressor Protein p53; D014779:Virus Replication
chemical_list: D017456:Adenovirus Early Proteins; D046911:Macromolecular Substances; D009363:Neoplasm Proteins; D009856:Oncogene Proteins, Viral; D010750:Phosphoproteins; D016159:Tumor Suppressor Protein p53