title: Concentration of dilute solutions of macromolecules by electrophoresis.
abstract: An electrophoresis apparatus which is used for concentrating micrograms of macromolecules from solutions as large as 250 ml is described. The recoveries were greater than 91% with three different macromolecules tested (28 to 360 kDa). Solutions with volumes in the range of 35 ml were concentrated 70-fold in less than 90 min to a final volume of 0.5 ml. Larger volumes in the range of 250 ml were concentrated 227-fold in 16 h to a final volume of 1.1 ml. Sterile concentrates can be obtained if the apparatus is constructed under sterile conditions.
authors.affiliation: Department of Allied Health Sciences, University of Science and Technology, Irbid, Jordan.
authors.forename: R F
authors.initials: RF
authors.lastname: Atmeh
mesh_terms: D000818:Animals; D016632:Apolipoprotein A-I; D001054:Apolipoproteins A / Q000032:analysis*; D002417:Cattle; D002627:Chemistry, Physical / Q000379:methods; D004586:Electrophoresis / Q000295:instrumentation*; D005069:Evaluation Studies as Topic; D006801:Humans; D008075:Lipoproteins, HDL / Q000032:analysis*; D046911:Macromolecular Substances; D012710:Serum Albumin, Bovine / Q000032:analysis*; D012996:Solutions
chemical_list: D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D008075:Lipoproteins, HDL; D046911:Macromolecular Substances; D012996:Solutions; D012710:Serum Albumin, Bovine