Amine oxidase released into plasma of rats treated with hepatotoxin allyl formate.
Amine oxidase activity in plasma of rats were investigated after pretreatment with the perilobular hepatotoxin allyl formate (AF). Amine oxidase activities in plasma elevated after administration of AF 0.1 ml/kg i.p. to male rats with 1 microM and 100 microM benzylamine (Bz), 10 microM beta-phenylethylamine (beta-PEA) and 100 microM serotonin (5-HT) as substrates. But the complete inhibition of amine oxidase activities with 5-HT and beta-PEA were not observed by clorgyline as A-form MAO inhibitor and deprenyl as beta-form MAO inhibitor. The deamination of 1 microM Bz was not inhibited at high concentrations of these MAO inhibitors, while it was inhibited at low concentrations of phenelzine and semicarbazide. On the other hand, the deamination of 100 microM Bz was highly sensitive with these MAO inhibitors, while it was less sensitive with phenelzine and semicarbazide as compared with 1 microM Bz. Then, the Km values of amine oxidase in plasma of AF-administered rats with Bz as substrate were determined from Lineweaver-Burk double reciprocal plots. Two Km values for Bz of high and low Bz concentration in amine oxidase in plasma of AF-administered rats were obtained. However, this Km value of low Bz concentration was not obtained from liver mitochondria and microsomes of control rat and AF-administered rats. The Km value for beta-PEA of MAO in plasma of AF-administered rats was the same as the values of rat liver mitochondrial MAO. These results indicate that native mitochondrial MAO was released from the liver, and two or more distinct amine oxidases were released from other organs in response to AF.
Obata
T
T
Department of Pharmacology, Medical College of Oita, Japan.
Egashira
T
T
Yamanaka
Y
Y
D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000201:enzymology*; D003000:Clonidine / Q000494:pharmacology; D005561:Formates / Q000633:toxicity*; D005563:Formic Acid Esters / Q000633:toxicity*; D007700:Kinetics; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D008930:Mitochondria, Liver / Q000201:enzymology; D008996:Monoamine Oxidase Inhibitors; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000037:antagonists & inhibitors / Q000097:blood*; D010624:Phenelzine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012642:Selegiline / Q000494:pharmacology; D012663:Semicarbazides / Q000494:pharmacology
D005561:Formates; D005563:Formic Acid Esters; D008996:Monoamine Oxidase Inhibitors; D012663:Semicarbazides; D012642:Selegiline; C034499:allyl formate; D000587:Oxidoreductases Acting on CH-NH Group Donors; D003000:Clonidine; D010624:Phenelzine