Atrial natriuretic factor inhibits angiotensin-induced aldosterone secretion: not through cGMP or interference with phospholipase C.
ANF did not prevent the formation of [3H] inositol trisphosphate in response to AII but inhibited aldosterone secretion in calf adrenal glomerulosa cells. 8-bromo cGMP did not affect either inositol phosphate formation or aldosterone secretion. Changes in cytosolic Ca++ concentration induced by AII, as measured by Quin 2 fluorescence, were also unaffected by ANF. No difference in adrenal cell protein phosphorylation with AII or AII + ANF was observed. The results suggest that ANF may inhibit aldosterone secretion through a non-guanyl cyclase linked receptor system not involving the formation of phosphoinositide-derived second messengers. Interference with protein kinase C activity cannot be ruled out.
Ganguly
A
A
Department of Medicine, University of South Florida College of Medicine, Tampa 33620.
Chiou
S
S
West
L A
LA
Davis
J S
JS
D000450:Aldosterone / Q000378:metabolism*; D000634:Aminoquinolines; D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D006152:Cyclic GMP / Q000031:analogs & derivatives / Q000494:pharmacology / Q000502:physiology*; D003600:Cytosol / Q000378:metabolism; D005456:Fluorescent Dyes; D015544:Inositol 1,4,5-Trisphosphate; D007295:Inositol Phosphates / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D010738:Type C Phospholipases / Q000378:metabolism*; D015384:Zona Glomerulosa / Q000187:drug effects / Q000378:metabolism*
D000634:Aminoquinolines; D005456:Fluorescent Dyes; D007295:Inositol Phosphates; D010750:Phosphoproteins; D000804:Angiotensin II; C016276:8-bromocyclic GMP; D000450:Aldosterone; D015544:Inositol 1,4,5-Trisphosphate; D009320:Atrial Natriuretic Factor; D010738:Type C Phospholipases; D006152:Cyclic GMP; C035739:Quin2; D002118:Calcium