Characterization of cerastobin, a thrombin-like enzyme from the venom of Cerastes vipera (Sahara sand viper).
Cerastobin, a thrombin-like enzyme, was isolated from the venom of Cerastes vipera (Sahara sand viper) in homogeneous form. Cerastobin had a molecular weight of 38,000 with 348 amino acid residues. It had an isoelectric point of 7.7 (a pH optimum of 7.9 and a temperature optimum of 45 degrees C). Cerastobin hydrolyzed arginine-containing synthetic substrates such as TAME, BAME, and BAEE, but BAPNA was not hydrolyzed. Cerastobin had thrombin-like activity, producing fibrin from fibrinogen and also hydrolyzing chromogenic substrates for thrombin such as 2AcOH.H-D-CHG-But-Arg-pNA (CBS 34.47) and H-D-Phe-Pip-Arg-pNA (S-2238). It showed kallikrein-like activity and hydrolyzed kallikrein substrates 2AcOH.H-D-Phe-Gly-Arg-pNA (CBS 33.27) and H-D-Pro-Phe-Arg-pNA (S-2302). It produced bradykinin from bradykininogen, as uterus contraction was observed. A serine inhibitor, DFP, exerted a pronounced inhibitory effect, suggesting that cerastobin is a serine-type protease. The sequence of 37 residues from the amino-terminal end was investigated. The amino-terminal amino acid was valine as it is in most other thrombin-like enzymes. The amino acid sequence of cerastobin was similar to that of thrombin in some residues and had some homology with that of kallikrein. However, cerastobin showed a high degree of homology to thrombin-like enzymes isolated from various snake venoms. Factor X was partially degraded by cerastobin. It was also found that antithrombin III was degraded by the enzyme. The alpha and beta chains of fibrin monomer were preferentially hydrolyzed by cerastobin, but the gamma chain was quite resistant.
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Department of Biochemistry, Colorado State University, Fort Collins 80523.
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D000595:Amino Acid Sequence; D001777:Blood Coagulation / Q000187:drug effects; D002265:Carboxylic Ester Hydrolases / Q000302:isolation & purification; D005337:Fibrin; D006801:Humans; D066298:In Vitro Techniques; D007526:Isoelectric Point; D007610:Kallikreins / Q000302:isolation & purification; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010974:Platelet Aggregation / Q000187:drug effects; D012697:Serine Endopeptidases / Q000302:isolation & purification* / Q000494:pharmacology; D013917:Thrombin / Q000302:isolation & purification; D014757:Viper Venoms / Q000302:isolation & purification* / Q000494:pharmacology
D014757:Viper Venoms; D005337:Fibrin; C019724:arginine esterase; D002265:Carboxylic Ester Hydrolases; D007610:Kallikreins; D012697:Serine Endopeptidases; C059344:cerastobin; D013917:Thrombin