title: Cloned endothelial cells from fetal bovine bone.
abstract: Primary cell cultures from fetal bovine sternum were developed in Coon's modified Ham's F-12 medium containing 10% Nu-Serum, 1% Ultroser-G, and 200 mg of galactose per liter. Clones were obtained by colony isolation; one clone, BBE-1, was selected for characterization. BBE-1 cells exhibited typical endothelial morphology by light and electron microscopy and immunofluorescence for factor VIII-related antigen throughout their life span of 8 months. The cells showed mitogenic responses to endothelial cell growth factor, basic fibroblast growth factor, insulin-like growth factor types I and II, platelet-derived growth factor, ascorbic acid, and progesterone. Parathyroid hormone stimulated intracellular accumulation of cAMP in BBE-1 cells but not in endothelial cells from two other tissues. These clonal cells provide a useful system for studies on bone vasculature, including its interactions with other bone cells.
authors.affiliation: Metabolic Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892.
authors.forename: E A
authors.initials: EA
authors.lastname: Streeten
authors.forename: R
authors.initials: R
authors.lastname: Ornberg
authors.forename: F
authors.initials: F
authors.lastname: Curcio
authors.forename: K
authors.initials: K
authors.lastname: Sakaguchi
authors.forename: S
authors.initials: S
authors.lastname: Marx
authors.forename: G D
authors.initials: GD
authors.lastname: Aurbach
authors.forename: M L
authors.initials: ML
authors.lastname: Brandi
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000166:cytology*; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002999:Clone Cells; D000242:Cyclic AMP / Q000378:metabolism; D004261:DNA Replication / Q000187:drug effects; D004727:Endothelium / Q000166:cytology* / Q000187:drug effects; D005333:Fetus; D006133:Growth Substances / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology; D007700:Kinetics
chemical_list: D006133:Growth Substances; D006493:Heparin; D000242:Cyclic AMP