Beta-endorphin in the brainstem and the cerebellum of the human infant: regional levels' profile assessed with immunoaffinity chromatography and solid phase radioimmunoassay.
The regional levels' profile of human beta-endorphin (beta h-EP) was studied in the brainstem and the cerebellum of 16 infant victims of "Sudden Infant Death Syndrome" and other death causes. An immunoaffinity chromatography procedure based on a monoclonal antibody directed specifically against the N-terminus of beta-EP was used to extract this peptide from the tissue samples. Beta-EP was then assessed quantitatively by means of a very sensitive solid phase radioimmunoassay (using a polyclonal antibody specific for the C-terminus of beta-EP) developed especially for the study presented here.
Pasi
A
A
Institute of Forensic Medicine, University of Zurich, Switzerland.
Kulling
P
P
Voellmy
D
D
Gramsch
C
C
Mehraein
P
P
Häni
M
M
Messiha
F S
FS
D001933:Brain Stem / Q000473:pathology*; D002531:Cerebellum / Q000473:pathology*; D002846:Chromatography, Affinity; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008526:Medulla Oblongata / Q000473:pathology; D008636:Mesencephalon / Q000473:pathology; D011149:Pons / Q000473:pathology; D011863:Radioimmunoassay; D013398:Sudden Infant Death / Q000473:pathology*; D001615:beta-Endorphin / Q000378:metabolism*
D001615:beta-Endorphin