title: Effects of chloroquine on release of mediators from mast cells.
abstract: This study was designed to investigate the effect of chloroquine (CQ) on the release of preformed and generated mediators from rat serosal mast cells (greater than 90% pure). CQ alone (10-1,000 micrograms/ml) induced the release of preformed mediators, while stimulation with calcium ionophore A23187 of CQ-treated cells resulted in suppressed releases of both preformed and generated (particularly prostaglandin D2) mediators. These effects were calcium-independent and reversible. Whether these results partly explain the efficacy of CQ in the treatment of mast cell-mediated dermatoses remains to be further investigated.
authors.affiliation: Dermatology Service, New York Veterans Administration Medical Center, N.Y.
authors.forename: K B
authors.initials: KB
authors.lastname: Green
authors.forename: H W
authors.initials: HW
authors.lastname: Lim
mesh_terms: D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects; D002738:Chloroquine / Q000494:pharmacology*; D003238:Connective Tissue / Q000378:metabolism; D003239:Connective Tissue Cells; D015777:Eicosanoids / Q000378:metabolism; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D012701:Serotonin / Q000378:metabolism
chemical_list: D001095:Arachidonic Acids; D015777:Eicosanoids; D007202:Indicators and Reagents; D016718:Arachidonic Acid; D012701:Serotonin; D000001:Calcimycin; D002738:Chloroquine; D002118:Calcium