[Transposition of insertional sequence IS21 in E. coli cells activated by an exogenous promotor].
Transpositional activity of the IS21-element was found to be activated during its cloning in the pUC18 vector plasmid. The activated IS-element restores ability to transposition of the deficient IS21-element present in the same cell. Simultaneously with the activated transposition the increase in frequencies of precise exclusion of IS-element is registered. The activation of IS-element is concluded to be directed by exogenous promoter.
Dubeĭkovskiĭ
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D004251:DNA Transposable Elements; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D015183:Restriction Mapping
D004251:DNA Transposable Elements
Aktiviruemaia vneshnim promotorom transpozitsiia insertsionnoĭ posledovatel'nosti IS21 v kletkakh E. coli.