title: Different T helper cell subsets elicited in mice utilizing two different adjuvant vehicles: the role of endogenous interleukin 1 in proliferative responses.
abstract: Mice were immunized with either poly(Glu, Arg, Ala) or poly(Glu, Lys, Phe) contained in two different adjuvant preparations, alum (A1K(SO4)2) or complete Freund's adjuvant (CFA), and in vitro antigen-driven proliferative responses of lymph node cells were assayed 4-16 days later. After immunization with antigens on alum, endogenous interleukin 1 (IL-1) as well as interleukin 4 (IL-4) production was required for the proliferation of the elicited T cells as inclusion of either polyclonal goat anti-mouse IL-1 alpha or monoclonal anti-mouse IL-4 (11B11) in the cultures inhibited proliferative responses to antigen. In contrast, proliferative responses of cells elicited by antigen in CFA were not inhibited by either anti-IL-1 or anti-IL-4. Monoclonal antimouse CD4 (GK 1.5) inhibited proliferative responses regardless of which adjuvant was used to elicit antigen-reactive cells. These data indicated that phenotypically different subpopulations of CD4+ cells were elicited by the same antigen administered in different adjuvant preparations, Th2-like cells after immunization with polymers on alum and Th1-like cells after immunization with antigens in CFA. An examination of the isotypes of polymer-specific antibodies present in the sera of immunized mice also supported this conclusion.
authors.affiliation: Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107.
authors.forename: J L
authors.initials: JL
authors.lastname: Grun
authors.forename: P H
authors.initials: PH
authors.lastname: Maurer
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D007114:Immunization; D007375:Interleukin-1 / Q000502:physiology*; D051379:Mice; D008815:Mice, Inbred Strains; D010455:Peptides / Q000008:administration & dosage / Q000276:immunology*; D014677:Pharmaceutical Vehicles; D011108:Polymers; D006377:T-Lymphocytes, Helper-Inducer / Q000145:classification / Q000502:physiology*
chemical_list: D000276:Adjuvants, Immunologic; D007375:Interleukin-1; D010455:Peptides; D014677:Pharmaceutical Vehicles; D011108:Polymers; C056210:glutamic acid-arginine-alanine polymer; C027143:poly(Glu(56)-Lys(35)-Phe(9))n