[The effect of temperature and Ca2+ on the interaction of high-density lipoproteins with epithelial cells in the human small intestine].
The effect of temperature and Ca2+ ions on the interaction of high-density lipoprotein (HDL3) with human enterocytes was studied. It was shown that Kd measured at 4 degrees C is similar to that at 37 degrees C. Maximal number of binding sites at 37 degrees C is 15-fold times higher than that at 4 degrees C. EDTA (10 mM) and CaCl2 (0.5-5 mM) did not affect binding and uptake of HDL3 by human enterocytes. The obtained results indicate that HDL3-binding with these cells depends on temperature and does not depend on Ca2+ ions.
Safonova
I G
IG
Sviridov
D D
DD
Men'shikov
G B
GB
Repin
V S
VS
D000293:Adolescent; D002122:Calcium Chloride / Q000494:pharmacology*; D002648:Child; D002675:Child, Preschool; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004492:Edetic Acid / Q000494:pharmacology; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007223:Infant; D007231:Infant, Newborn; D007421:Intestine, Small / Q000187:drug effects* / Q000378:metabolism; D007457:Iodine Radioisotopes; D008075:Lipoproteins, HDL / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects / Q000502:physiology; D013696:Temperature
D007457:Iodine Radioisotopes; D008075:Lipoproteins, HDL; D004492:Edetic Acid; D002122:Calcium Chloride
Vliianie temperatury i Ca2+ na vzaimodeĭstvie lipoproteidov vysokoĭ plotnosti s kletkami épiteliia tonkogo kishechnika cheloveka.