Construction and characterization of a packaging cell line for MMTV-based conditional retroviral vectors.
A chimeric provirus in which the 5'LTR of a complete biologically active Mouse Mammary Tumour Virus (MMTV) proviral DNA has been replaced with the Rous Sarcoma Virus LTR has been constructed. Upon transfection into permissive cells, this provirus directs the synthesis of the MMTV gag and env structural proteins, but is impaired in packaging of the RNAs that encode these proteins. Supertransfection of these cells with MMTV based vector constructs results in the production of infectious recombinant virus at a higher efficiency than with previously described helper cell lines. Such a retroviral vector system based on MMTV will allow the study of the effects of conditional expression of inserted genes upon infected cells.
Salmons
B
B
Department of Cell and Molecular Biology, Medical College of Georgia, Augusta 30912.
Moritz-Legrand
S
S
Garcha
I
I
Günzburg
W H
WH
D001358:Avian Sarcoma Viruses / Q000235:genetics*; D001483:Base Sequence; D002460:Cell Line; D002999:Clone Cells; D015683:Gene Products, gag; D005822:Genetic Vectors; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D012191:Retroviridae Proteins / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection; D014759:Viral Envelope Proteins / Q000235:genetics; D014771:Virion / Q000235:genetics
D015683:Gene Products, gag; D012191:Retroviridae Proteins; D014759:Viral Envelope Proteins