Enzyme histochemical investigation of ITO's cells in semithin sections of human liver biopsy specimens.
Human liver specimens obtained by needle biopsy were embedded into glycolmethacrylate and enzyme histochemical investigations were carried out on semithin sections. By this method, we could demonstrate an activity of acid phosphatase (EC 3.1.3.2) by azo-coupling as well as by metal precipitation methods, of alkaline phosphatase (EC 3.1.3.1) by metal precipitation method, and of ATPases (EC 3.6.1.3 and EC 3.6.1.4) in ITO's cells. Special aspects of the application of enzyme histochemical methods to resin-embedded liver tissue are explained.
Körner
T
T
Department of Internal Medicine, Martin Luther University Halle-Wittenberg, GDR.
Rath
F W
FW
D000135:Acid Phosphatase / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000378:metabolism*; D001706:Biopsy; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D006651:Histocytochemistry; D006801:Humans; D008099:Liver / Q000166:cytology / Q000201:enzymology*
D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases