Detection of T cells in paraffin wax embedded tissue using antibodies against a peptide sequence from the CD3 antigen.
Rabbit polyclonal antibodies were raised against a proline rich, peptide sequence, comprising 13 amino acids, in the cytoplasmic domain of the CD3 epsilon chain. Immunoprecipitation experiments showed that this antibody preparation recognised the CD3 antigen on human T lymphoblasts. The antibody stained normal T cells strongly in tissue sections which had been fixed in formalin or Bouin's solution and embedded in paraffin wax. Its reactivity with T cell lymphoma, when evaluated on a series of 96 previously phenotyped cases, closely agreed with the results obtained on cryostat sections. These results indicate that the specific detection of T cells in routinely processed tissue biopsy specimens is now technically feasible on a wide scale in diagnostic laboratories using CD3 peptide antibodies, and they also suggest that in future the use of anti-peptide antibodies may detect other lineage specific antigenic markers in paraffin wax sections.
Mason
D Y
DY
Department of Pathology, John Radcliffe Hospital, Headington, Oxford.
Cordell
J
J
Brown
M
M
Pallesen
G
G
Ralfkiaer
E
E
Rothbard
J
J
Crumpton
M
M
Gatter
K C
KC
D000595:Amino Acid Sequence; D000906:Antibodies / Q000276:immunology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D014408:Biomarkers, Tumor / Q000032:analysis; D017252:CD3 Complex; D006801:Humans; D007124:Immunoenzyme Techniques; D008223:Lymphoma / Q000473:pathology; D008562:Membrane Glycoproteins / Q000276:immunology; D008969:Molecular Sequence Data; D010232:Paraffin; D010446:Peptide Fragments / Q000276:immunology; D011233:Precipitin Tests; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D013601:T-Lymphocytes; D014021:Tissue Preservation; D014885:Waxes
D000906:Antibodies; D000945:Antigens, Differentiation, T-Lymphocyte; D014408:Biomarkers, Tumor; D017252:CD3 Complex; D008562:Membrane Glycoproteins; D010446:Peptide Fragments; D011948:Receptors, Antigen, T-Cell; D014885:Waxes; D010232:Paraffin