Direct isolation (without enrichment) of Listeria monocytogenes in milk.
In the present work we have developed a direct method (without enrichment) for the isolation of listeriae in milk, using the combinated action of SDS (sodium dodecyl sulphate), centrifugation and selective media. It is possible to identify even 0.5 listeriae/ml amongst 7.2 x 10(7) c.f.u. contaminants/ml.
Dominguez
L
L
Department of Animal Pathology, Microbiology and Immunity, Veterinary Faculty, Madrid, Spain.
Briones
V
V
Blanco
M
M
Fernandez-Garayzabal
J F
JF
Blanco
J L
JL
Vazquez
J A
JA
Suarez
G
G
D000818:Animals; D001386:Azides / Q000494:pharmacology; D001431:Bacteriological Techniques; D002498:Centrifugation; D003470:Culture Media; D005516:Food Microbiology; D008089:Listeria monocytogenes / Q000187:drug effects / Q000302:isolation & purification*; D008892:Milk / Q000382:microbiology*; D019810:Sodium Azide; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology
D001386:Azides; D003470:Culture Media; D012967:Sodium Dodecyl Sulfate; D019810:Sodium Azide