Glycosphingolipids of human urothelial cell lines with different grades of transformation.
Neutral glycolipids and gangliosides from seven human urothelial cell lines, differing in grades of transformation (TGr), were characterized by fast atom bombardment mass spectrometry, exoglycosidase treatment and an immunostaining procedure. The major neutral glycolipids identified in all cell lines studied included CMH, CDH, CTH, globoside and paragloboside, the gangliosides were GM3, GM2, sialosylparagloboside and GD1a. The following observations were made: 1. GM2 was the major ganglioside in the TGrII cell lines (non-tumorigenic, non-invasive), but a minor component in the TGrIII cell lines (tumorigenic, invasive). 2. All components showed C16:0 and C24:0 as major fatty acids, but in the TGrIII cell lines the fatty acid composition of CMH and some of the gangliosides were more complex showing unsaturated and hydroxy-fatty acids as well.
Ugorski
M
M
Department of Immunochemistry, Polish Academy of Sciences, Wroclaw.
Påhlsson
P
P
Dus
D
D
Nilsson
B
B
Radzikowski
C
C
D000911:Antibodies, Monoclonal; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002250:Carbon Radioisotopes; D002295:Carcinoma, Transitional Cell; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004848:Epithelium; D005690:Galactose / Q000378:metabolism; D005732:Gangliosides / Q000737:chemistry / Q000302:isolation & purification; D006026:Glycoside Hydrolases; D006028:Glycosphingolipids / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D015151:Immunoblotting; D008969:Molecular Sequence Data; D011865:Radioisotope Dilution Technique; D016339:Spectrometry, Mass, Fast Atom Bombardment; D001743:Urinary Bladder; D001749:Urinary Bladder Neoplasms
D000911:Antibodies, Monoclonal; D002250:Carbon Radioisotopes; D005732:Gangliosides; D006028:Glycosphingolipids; D006026:Glycoside Hydrolases; D005690:Galactose