Human IgE response: virus-activated IgE secretors are interleukin-2-dependent cells.
The activation of human IgE-secreting B lymphocytes was difficult until the use of low cell density limiting dilution cultures, which are more efficient and eliminate regulatory growth restraints, became possible. The transformation of IgE-producing cells by Epstein-Barr virus unexpectedly required the presence of small supplements of mature T lymphocytes early in culture. Interleukin 2 (IL2, 10 U/ml) was able to completely replace T-cell supplements when added during a brief (1-2 cell cycles) but critical period, 5-8 days after infection with the virus. The observation that IgE-committed B cells are principally able to express receptors for and respond to IL2 is of experimental utility and implies the possibility that this T-cell product could be part of the complex T-cell controls which govern IgE-committed B cells.
Chan
M A
MA
Division of Immunology/Rheumatology, Hospital for Sick Children, Research Institute, Toronto, Canada.
Dosch
H M
HM
D000328:Adult; D001402:B-Lymphocytes / Q000276:immunology*; D002472:Cell Transformation, Viral; D004854:Herpesvirus 4, Human / Q000502:physiology; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology / Q000378:metabolism*; D007132:Immunoglobulin Isotypes / Q000096:biosynthesis; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000502:physiology*; D013601:T-Lymphocytes / Q000502:physiology
D007132:Immunoglobulin Isotypes; D007376:Interleukin-2; D007073:Immunoglobulin E