Utilization of 2'-deoxynad for ADP-ribose transfer reactions.
2'-deoxyNAD was examined as a substrate for both mono(ADP-ribosyl)ation and poly(ADP-ribosyl)ation reactions. 2'-deoxyNAD is a substrate for the diphtheria toxin-catalyzed mono(ADP-ribosyl)ation of elongation factor-2, inactivating its function to enhance protein synthesis. On the other hand, 2'-deoxyNAD is a poor substrate for poly(ADP-ribose) polymerase. 2'-deoxyNAD was not synthesized intracellularly from deoxyATP, even when deoxyATP content was markedly increased by incubation of cells with deoxyadenosine and an adenosine deaminase inhibitor. 2'-deoxyNAD, because of its specificity, could be a quite useful reagent for the investigation of cellular mono(ADP-ribosyl)ation reactions.
Wasson
D B
DB
Department of Basic and Clinical Research, Scripps Clinic & Research Foundation, La Jolla, California.
Yamanaka
H
H
Carson
D A
DA
D036002:ADP Ribose Transferases; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D004167:Diphtheria Toxin / Q000378:metabolism*; D006801:Humans; D009243:NAD / Q000138:chemical synthesis / Q000378:metabolism / Q000031:analogs & derivatives; D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors / Q000378:metabolism*; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*
D004167:Diphtheria Toxin; D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D009243:NAD; D000246:Adenosine Diphosphate Ribose; C021004:2'-deoxynicotinamide adenine dinucleotide; D036002:ADP Ribose Transferases; D011065:Poly(ADP-ribose) Polymerases