title: Demonstration of surface antigens on bronchoalveolar lavage cells using the immunoalkaline phosphatase method.
abstract: The immunoalkaline phosphatase procedure is described as a method for labelling bronchoalveolar lavage cellular specimens with monoclonal antibodies. This method has several advantages over conventional immunofluorescent techniques: it can be performed on cytocentrifuge preparations stored for long periods before staining; cell morphology can be observed in detail in positive and negative cells; the staining is permanent and stable, and, the reaction can be evaluated with a light microscope. Normal values for lymphocyte subpopulations in smokers and nonsmokers are also reported.
authors.affiliation: Servicio de Neumología y Hospital Clinic, Barcelona, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Xaubet
authors.forename: C
authors.initials: C
authors.lastname: Agusti
authors.forename: C
authors.initials: C
authors.lastname: Picado
authors.forename: A
authors.initials: A
authors.lastname: Urbano-Ispizua
authors.forename: M
authors.initials: M
authors.lastname: Carrión
authors.forename: A
authors.initials: A
authors.lastname: Agustí-Vidal
mesh_terms: D000328:Adult; D000469:Alkaline Phosphatase; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis*; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques / Q000592:standards*; D008297:Male; D008875:Middle Aged; D012907:Smoking / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000032:analysis; D050378:T-Lymphocytes, Regulatory / Q000032:analysis
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000469:Alkaline Phosphatase