title: Two-dimensional gel electrophoresis of RNase T1 resistant oligonucleotides of flavivirus RNA using ultrathin gels.
abstract: A convenient method employing a commercially available apparatus for two-dimensional gel electrophoresis of RNase T1 resistant oligonucleotides using ultrathin gels has been developed. The methodology overcomes problems commonly associated with the establishment of good, bubble-free, fusion of the first and second dimension gels. The use of ultrathin gels results in autoradiograms with well resolved oligonucleotide spots.
authors.affiliation: Department of Microbiology, University of Western Australia, Nedlands.
authors.forename: R J
authors.initials: RJ
authors.lastname: Coelen
authors.forename: L M
authors.initials: LM
authors.lastname: Flynn
authors.forename: J S
authors.initials: JS
authors.lastname: Mackenzie
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005416:Flavivirus / Q000302:isolation & purification*; D012367:RNA, Viral / Q000302:isolation & purification*; D006163:Ribonuclease T1; D014773:Virology / Q000379:methods
chemical_list: D012367:RNA, Viral; D006163:Ribonuclease T1