Labelling oligonucleotides to high specific activity (I).
The normal procedure for labelling oligonucleotides radioactively is the use of polynucleotide kinase and gamma 32P-ATP. However, this has the disadvantage of only introducing one labelled base per molecule of the oligonucleotide. In this paper we describe an approach based on primer/template combinations using conventional fill-in conditions followed by the release of the labelled sequence by digestion with uracil-DNA glycosylase.
Craig
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AG
Genome Analysis Laboratory, Imperial Cancer Research Fund Laboratories, London, UK.
Nizetic
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Lehrach
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D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D004279:DNA, Viral / Q000378:metabolism; D015336:Molecular Probe Techniques; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000138:chemical synthesis / Q000378:metabolism; D013698:Templates, Genetic
D004279:DNA, Viral; D015345:Oligonucleotide Probes