Glycine modulation of NMDA-evoked release of [3H]acetylcholine and [3H]dopamine from rat striatal slices.
Superfused slices of rat striatum were used to investigate whether N-methyl-D-aspartate (NMDA) receptors mediating [3H]acetylcholine (ACh) and [3H]dopamine (DA) release are modulated by glycine. Kynurenic acid, a compound possessing antagonist activity at the receptor's glycine modulatory site, dose-dependently blocked NMDA release of both transmitters. The inhibition was reversed by glycine, D-serine, D-alanine, D-cysteine, L-serine and L-alanine. The relative potencies of these amino acids was in agreement with their previously reported abilities to inhibit strychnine-insensitive [3H]glycine binding and enhance [3H]MK-801 binding to the NMDA receptor complex. These data indicate that glycine modulatory sites are linked to NMDA receptors involved in striatal [3H]ACh and [3H]DA release.
Ransom
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Merck Sharp and Dohme Research Laboratories, West Point, PA 19486.
Deschenes
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D000109:Acetylcholine / Q000378:metabolism*; D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D003986:Dibenzocycloheptenes / Q000494:pharmacology; D016291:Dizocilpine Maleate; D004298:Dopamine / Q000378:metabolism*; D005998:Glycine / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D016202:N-Methylaspartate; D051381:Rats; D011919:Rats, Inbred Strains; D016194:Receptors, N-Methyl-D-Aspartate; D017981:Receptors, Neurotransmitter / Q000187:drug effects / Q000502:physiology*; D013779:Tetrodotoxin / Q000494:pharmacology
D000596:Amino Acids; D003986:Dibenzocycloheptenes; D016194:Receptors, N-Methyl-D-Aspartate; D017981:Receptors, Neurotransmitter; D001224:Aspartic Acid; D013779:Tetrodotoxin; D016202:N-Methylaspartate; D016291:Dizocilpine Maleate; D000109:Acetylcholine; D005998:Glycine; D004298:Dopamine