Modulation of cytochrome P450 isozymes in human liver, by ethanol and drug intake.
Cytochromes P450 (P450) are a family of isozymes which play an important role in xenobiotic metabolism. The concentration of three P450 isozymes, namely P450-IIE1(A1c),-IIIA(NF) and -IIC8-10(MP) has been measured in human liver biopsies of patients with different alcohol and drug intake status. All these three P450s were expressed in all subjects. Ethanol intake increased P450-IIE1(A1c) content with no effect on the content of the two other P450s. Drug intake (barbiturates) increased both P450-IIIA(NF) and -IIC8-10(MP) content without any effect on P450-IIE1(A1c). This paper brought, at protein level, further evidence of the importance of environmental conditions on P450 isozyme pattern, and therefore, on drug metabolizing capacity of human liver.
Perrot
N
N
INSERM U 75, CHU Necker, Paris, France.
Nalpas
B
B
Yang
C S
CS
Beaune
P H
PH
D000328:Adult; D000428:Alcohol Drinking / Q000502:physiology*; D000437:Alcoholism / Q000201:enzymology; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D013695:Temperance; D013997:Time Factors
D007527:Isoenzymes; D003577:Cytochrome P-450 Enzyme System