The role of the carboxy-terminal membrane-spanning fragment in the biogenesis of Escherichia coli K12 outer membrane protein PhoE.
PhoE protein of Escherichia coli K12 is an outer membrane protein which is supposed to span the membrane sixteen times. By creating a deletion which removes the last membrane-spanning fragment and studying the localization of the truncated PhoE, we show that this fragment is indispensable for trimerization and outer membrane localization. In addition, circumstantial evidence for the proposed topology model of the protein was obtained. An insertion mutation in a region supposed to be cell surface-exposed, interferes with the binding of a monoclonal antibody which recognizes a cell surface-exposed epitope of the protein.
Bosch
D
D
Department of Molecular Cell Biology, State University of Utrecht, The Netherlands.
Scholten
M
M
Verhagen
C
C
Tommassen
J
J
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002872:Chromosome Deletion; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D007150:Immunohistochemistry; D009154:Mutation; D018272:Porins; D011487:Protein Conformation; D013347:Subcellular Fractions / Q000378:metabolism
D001425:Bacterial Outer Membrane Proteins; D018272:Porins