title: Expression of the rtp gene of Bacillus subtilis is required for replication fork arrest at the chromosome terminus.
abstract: It was earlier proposed that clockwise replication fork arrest at the chromosome terminus in Bacillus subtilis is dependent upon expression of the rtp gene adjacent to the site of arrest, terC [Smith and Wake, J. Bacteriol. 170 (1988) 4083-4090]. A merodiploid strain of B. subtilis, in which rtp was placed under the control of the IPTG-inducible spac-1 promoter, was constructed. Replication fork arrest at terC, as monitored by the level of a forked DNA molecule of predicted dimensions, was shown to be dependent upon IPTG-induced expression of rtp in this strain. The very low concentration of IPTG needed to induce a substantial level of fork arrest suggests that relatively little RTP, the protein product of rtp, is needed for fork arrest at terC.
authors.affiliation: Department of Biochemistry, University of Sydney, N.S.W., Australia.
authors.forename: M T
authors.initials: MT
authors.lastname: Smith
authors.forename: R G
authors.initials: RG
authors.lastname: Wake
mesh_terms: D001412:Bacillus subtilis / Q000187:drug effects / Q000235:genetics*; D001426:Bacterial Proteins; D015139:Blotting, Southern; D002876:Chromosomes, Bacterial / Q000502:physiology*; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects; D005798:Genes, Bacterial; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C063112:rtP protein, Bacillus subtilis; D007544:Isopropyl Thiogalactoside