Antibodies to CD4 in individuals infected with human immunodeficiency virus type 1.
The attachment of human immunodeficiency virus type 1 (HIV-1) to target cells is mediated by a specific interaction between the viral envelope glycoprotein (gp120) and the CD4 receptor. Here we report that approximately 10% of HIV-1-infected individuals produce antibodies that recognize the extracellular portion of the CD4 molecule. Carboxyl-terminal deletions of CD4 that do not affect HIV-1 gp120 binding eliminate recognition of CD4 by patient antisera. In contrast, mutations in the amino-terminal domain of CD4 that attenuate HIV-1 gp120 binding do not diminish CD4 recognition by patient antisera. These results suggest that HIV-1 infection can generate antibodies directed against a region of the viral receptor distinct from the virus-binding domain.
Kowalski
M
M
Division of Human Retrovirology, Dana-Farber Cancer Institute, Boston, MA 02115.
Ardman
B
B
Basiripour
L
L
Lu
Y C
YC
Blohm
D
D
Haseltine
W
W
Sodroski
J
J
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology*; D000595:Amino Acid Sequence; D000954:Antigens, Surface; D001323:Autoantibodies / Q000032:analysis*; D001665:Binding Sites; D003062:Codon; D004247:DNA / Q000235:genetics; D015699:HIV Envelope Protein gp120; D006679:HIV Seropositivity; D015497:HIV-1; D006801:Humans; D007163:Immunosorbent Techniques; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids; D015728:Receptors, HIV; D011991:Receptors, Virus / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D011994:Recombinant Proteins; D012191:Retroviridae Proteins / Q000378:metabolism; D014162:Transfection; D014759:Viral Envelope Proteins
D000954:Antigens, Surface; D001323:Autoantibodies; D003062:Codon; D015699:HIV Envelope Protein gp120; D015728:Receptors, HIV; D011991:Receptors, Virus; D011994:Recombinant Proteins; D012191:Retroviridae Proteins; D014759:Viral Envelope Proteins; D004247:DNA