The regulation of amyloid beta protein precursor secretion and its modulatory role in cell adhesion.
The regulation and function of two forms of the amyloid beta protein precursor (ABPP) that are released into the growth-conditioned medium of the PC12 nerve cell line were examined. Nerve growth factor increases the release of the form of ABPP without the protease-inhibitor domain relative to the protein containing the protease inhibitor and increases the overall rate of ABPP secretion 2-fold. In contrast, fibroblast growth factor increases the rate of ABPP secretion approximately 7-fold. Both forms of the secreted ABPP molecule are, in turn, able to stimulate adhesion of PC12 cells to substrata to which they are adsorbed about 10-fold more efficiently on a molar basis than Iaminin.
Schubert
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D
Salk Institute For Biological Studies, San Diego, California 92138.
Jin
L W
LW
Saitoh
T
T
Cole
G
G
D000682:Amyloid / Q000378:metabolism* / Q000494:pharmacology / Q000502:physiology; D016564:Amyloid beta-Protein Precursor; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D008970:Molecular Weight; D009414:Nerve Growth Factors / Q000494:pharmacology; D009474:Neurons / Q000378:metabolism / Q000502:physiology*; D011480:Protease Inhibitors; D011498:Protein Precursors / Q000378:metabolism* / Q000494:pharmacology / Q000502:physiology
D000682:Amyloid; D016564:Amyloid beta-Protein Precursor; D009414:Nerve Growth Factors; D011480:Protease Inhibitors; D011498:Protein Precursors; D005346:Fibroblast Growth Factors