title: Structural organization of the gene encoding apolipoprotein A-II in an amyloidotic strain of senescence-accelerated mouse.
abstract: An inherited polymorphism occurring in the murine apolipoprotein A-II (ApoA-II) transcript seems to be related to the senile amyloidosis which occurs in accelerated-senescence-prone mice (SAM-P). Such being the case, we have determined the entire nucleotide (nt) sequence of the apoA-II gene. The length of the gene is about 1.3 kb and it is interrupted by three introns and the four exons aligned perfectly with the previously sequenced elements of an apoA-II cDNA. Two-nt substitutions [Pro-5(CCA)----Gln(CAG)] in the SAM-P genome were identified in the third exon, hence, we could use a restriction fragment length polymorphism to detect the apoA-II molecular type. Several possible regulatory signals were identified (i) in the 5'-flanking region, including CAAT and TATA boxes, the viral enhancer-like sequence, and the consensus sequences of estrogen response element, and (ii) in the 3'-flanking region, including sequences conserved in the immunoglobulin enhancer, glucocorticoid and estrogen response elements, and a B1 repetitive sequence.
authors.affiliation: Gerontology and Nutrition Division, Meiji Institute of Health Science, Odawara, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yonezu
authors.forename: M
authors.initials: M
authors.lastname: Toda
authors.forename: H
authors.initials: H
authors.lastname: Yamagishi
authors.forename: K
authors.initials: K
authors.lastname: Higuchi
authors.forename: T
authors.initials: T
authors.lastname: Takeda
mesh_terms: D000595:Amino Acid Sequence; D000686:Amyloidosis / Q000235:genetics*; D000818:Animals; D016633:Apolipoprotein A-II; D001054:Apolipoproteins A / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004195:Disease Models, Animal; D005796:Genes; D006801:Humans; D008075:Lipoproteins, HDL / Q000235:genetics*; D051379:Mice; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D016633:Apolipoprotein A-II; D001054:Apolipoproteins A; D008075:Lipoproteins, HDL