Comparison of amino acid sequence analysis by electron ionization and negative-ion chemical ionization mass spectrometry.
The positive electron ionization and negative chemical ionization mass spectra of 15 different derivatives of the tripeptide Phe-Ala-Leu have been compared. Total ion currents and ion currents of sequence-characterizing ions have been measured and compared. The negative-ion spectra, using 10% carbon dioxide in argon as moderator gas, proved to be simpler and contained more abundant sequence ions than the positive electron ionization spectra.
Reinhard
H
H
Institute of Organic Chemistry, University of Bern, Switzerland.
Lauber
R
R
Schlunegger
U P
UP
D000595:Amino Acid Sequence; D013058:Mass Spectrometry / Q000379:methods*