Non-equilibrium pH gradient electrophoresis (NEPHGE) on ultrathin polyacrylamide gels containing separators: improved erythrocyte phosphoglucomutase (PGM) and esterase D (EsD) diagnosis in red cell lysates and bloodstains.
Two rapid and reliable electrophoretic techniques for PGM1 and EsD typing on ultrathin polyacrylamide gels are described. They have been based on non-equilibrium pH gradient electrophoresis and on the addition of chemical spacers (EPPS for PGM1 and HEPES for EsD) to the gel mixture.
Destro-Bisol
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Istituto di Medicina Legale e delle Assicurazioni dell'Universitá Cattolica del Sacro Cuore, Italy.
Spinella
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A
D001801:Blood Stains; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000097:blood*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010641:Phenotype; D010733:Phosphoglucomutase / Q000097:blood*
D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; C020118:ESD protein, human; D010733:Phosphoglucomutase