Clinical and microbiologic findings in lambs inoculated with Pasteurella haemolytica after infection with ovine adenovirus type 6.
Colostrum-deprived lambs (10 to 20 days old) were inoculated with either ovine adenovirus type 6 (OAV-6; n = 6), Pasteurella haemolytica type A1 (n = 6), or OAV-6 followed by P haemolytica 5 days later (n = 10). Another group (n = 3) served as sham-inoculated controls. Lambs inoculated with OAV-6 or P haemolytica developed mild and moderate respiratory tract disease of 6 and 3 days' duration, respectively. Lambs inoculated with virus and bacteria developed clinical signs of respiratory tract disease of greater intensity and duration (9 days) than with either agent alone. Within 3 hours of bacterial inoculation, all lambs that received P haemolytica were anorectic, listless, and febrile, and had hyperpnea and dyspnea. Ovine adenovirus type 6 was isolated from all virus-inoculated lambs. Although P haemolytica was not recovered from all bacteria-inoculated lambs, it was recovered for a longer period in the group that received both agents. Antibody to OAV-6 was detected in virus-inoculated lambs as early as day 6 after inoculation. The control lambs remained clinically normal and neither virus nor bacteria were recovered at necropsy.
Lehmkuhl
H D
HD
National Animal Disease Center, USDA, Ames, Iowa 50010.
Contreras
J A
JA
Cutlip
R C
RC
Brogden
K A
KA
D000256:Adenoviridae / Q000302:isolation & purification; D000257:Adenoviridae Infections / Q000150:complications / Q000382:microbiology / Q000503:physiopathology / Q000662:veterinary*; D000818:Animals; D001831:Body Temperature; D010325:Pasteurella / Q000302:isolation & purification; D010326:Pasteurella Infections / Q000150:complications / Q000382:microbiology / Q000503:physiopathology / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology / Q000503:physiopathology; D013997:Time Factors