pH sensitivity of the thrombin-induced rise in fructose 2,6-bisphosphate content of human platelets.
The stimulation of human platelets with thrombin results in a rapid and sustained increase in the fructose 2,6-bisphosphate content which may play an important role in the potentiation of glycolytic flux induced by the agonist. The investigation of the effect of pH on thrombin-induced rise in platelet fructose 2,6-bisphosphate content is reported here. The results indicate that the early intracellular alkalinization which follows platelet stimulation may contribute to mediate the positive effect of thrombin on the regulatory metabolite.
Vasta
V
V
Department of Biochemistry, University of Florence, Italy.
Bruni
P
P
Meacci
E
E
Farnararo
M
M
D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D002352:Carrier Proteins / Q000097:blood; D005635:Fructosediphosphates / Q000097:blood*; D006598:Hexosediphosphates / Q000097:blood*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008985:Monensin / Q000494:pharmacology; D017923:Sodium-Hydrogen Exchangers; D013917:Thrombin / Q000494:pharmacology*
D002352:Carrier Proteins; D005635:Fructosediphosphates; D006598:Hexosediphosphates; D017923:Sodium-Hydrogen Exchangers; C027652:fructose 2,6-diphosphate; D008985:Monensin; D013917:Thrombin