[The MTT-dye test for the in vitro vitality control of fresh as opposed to cryopreserved rat pancreatic islets for syngeneic intraportal islet transplantation].
The vitality of freshly isolated and of cryopreserved (-1 degree C/min, 10% DMSO) islets of the rat pancreas was determined by means of the colorimetric MTT-test. The recover rate of vital islet cells amounted to 35% after cryopreservation. The in vitro results were confirmed by the effects after syngeneic intraportal transplantation. The transplantation of freshly isolated islets of Langerhans leads to a permanent normoglycemia in Streptozotocin diabetic rats (n = 5). Cryopreserved islets after transplantation (n = 5) led in contrary to it to no influence of the metabolism. The hepatic insulin content of livers with implanted cryopreserved islets amounted only 43% in face of livers with transplantation of freshly isolated islets. The MTT-assay allows accordingly a prompt judgement of the quality of islet allotransplants and this should make its way in the clinical practice.
Gütte
N
N
Chirurgische Klinik, Bereichs Medizin (Charité) der Humboldt-Universität zu Berlin.
Kühn
F
F
Matthes
G
G
Georgi
K
K
Gronau
K
K
Braun
K
K
D000818:Animals; D000831:Animals, Newborn; D003124:Colorimetry; D015925:Cryopreservation; D003921:Diabetes Mellitus, Experimental / Q000601:surgery*; D007328:Insulin / Q000032:analysis; D016381:Islets of Langerhans Transplantation; D008099:Liver / Q000032:analysis; D009926:Organ Preservation; D051381:Rats; D011917:Rats, Inbred Lew
D007328:Insulin
MTT-Farbtest für die in vitro-Vitalitätskontrolle frischer und kryokonservierter Ratteninseln im Vergleich zur syngenen intraportalen Inseltransplantation.