title: Transfer of a protein encoded by a single nucleus to nearby nuclei in multinucleated myotubes.
abstract: Specialized regions of muscle fibers may result from differential gene expression within a single fiber. In order to investigate the range of action of individual nuclei in multinucleated myotubes, C2 myoblasts were transfected to obtain stable cell lines that express a reporter protein that is targeted to the nucleus. Hybrid myotubes were then formed containing one or a few transfected nuclei as well as a large number of nuclei from the parental strain. In order to determine how far the products of a single nucleus extend, transfected nuclei were labeled with [3H]thymidine before fusion and the myotubes were stained to identify the reporter protein. In such myotubes the fusion protein was not confined to its nucleus of origin, but was restricted to nearby nuclei.
authors.affiliation: Department of Physiology, School of Medicine, University of California, San Francisco 94143-0444.
authors.forename: E
authors.initials: E
authors.lastname: Ralston
authors.forename: Z W
authors.initials: ZW
authors.lastname: Hall
mesh_terms: D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000378:metabolism; D004742:Enhancer Elements, Genetic; D004926:Escherichia coli / Q000235:genetics; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D005914:Globins / Q000235:genetics; D051379:Mice; D009124:Muscle Proteins / Q000235:genetics* / Q000378:metabolism; D009132:Muscles / Q000648:ultrastructure*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011965:Receptors, Glucocorticoid / Q000235:genetics; D013539:Simian virus 40 / Q000235:genetics; D014162:Transfection; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D009124:Muscle Proteins; D011965:Receptors, Glucocorticoid; D005914:Globins; D001616:beta-Galactosidase