Inositol trisphosphate induces calcium release from Neurospora crassa vacuoles.
Inositol 1,4,5-trisphosphate is known to release calcium ions from intracellular stores thought to be parts of endoplasmic reticulum in animal cells. In Neurospora crassa, however, inositol 1,4,5-trisphosphate acts on vacuoles stimulating a calcium efflux with a Km of 5.28 microM. The calcium release is inhibited effectively by dantrolene. These results were obtained by applying two independent methods, measuring calcium binding to fura-2 and loading vacuoles with 45Ca.
Cornelius
G
G
Institute for Genetics, Saarland University, Saarbrücken, Fed. Rep. Germany.
Gebauer
G
G
Techel
D
D
D001572:Benzofurans; D002118:Calcium / Q000378:metabolism*; D002132:Calcium Radioisotopes; D003620:Dantrolene / Q000494:pharmacology; D005456:Fluorescent Dyes; D016257:Fura-2; D015544:Inositol 1,4,5-Trisphosphate; D007295:Inositol Phosphates / Q000494:pharmacology*; D009491:Neurospora / Q000648:ultrastructure*; D009492:Neurospora crassa / Q000187:drug effects / Q000648:ultrastructure*; D013050:Spectrometry, Fluorescence; D013403:Sugar Phosphates / Q000494:pharmacology*; D014617:Vacuoles / Q000187:drug effects / Q000378:metabolism*
D001572:Benzofurans; D002132:Calcium Radioisotopes; D005456:Fluorescent Dyes; D007295:Inositol Phosphates; D013403:Sugar Phosphates; D015544:Inositol 1,4,5-Trisphosphate; D003620:Dantrolene; D002118:Calcium; D016257:Fura-2