Effect of intraperitoneal injection of mitomycin C adsorbed on activated carbon particles on induction of cytolytic peritoneal macrophages of mice.
The effects of the intraperitoneal injection of mitomycin C adsorbed onto activated carbon particles (MMC-CH) were assessed on the host immune status and the induction of cytolytic peritoneal macrophages (PM) in mice in comparison with an aqueous MMC solution (MMC-AQ). PM from inbred C57BL/6 mice and syngeneic B16 melanoma cells were used as the effector and target cells for the PM-mediated cytotoxicity assay. After a single injection of MMC-AQ (at the dose of 1/2 LD50), the weights of thymus and spleen and the 3H-thymidine uptake of spleen cells stimulated by concanavalin A were markedly decreased compared to those following injection of MMC-CH containing the same dose of MMC. A significant difference was found in the chronological changes of peritoneal exudate cell (PEC) numbers and PM cytolytic activity between the MMC-AQ- and MMC-CH-treated mice. One day after MMC-AQ injection, the PEC numbers were decreased markedly. However, they increased ten-fold after MMC-CH injection and the PM cytolytic activity was significantly higher after MMC-CH injection than after MMC-AQ injection during the first 3 days. The present results suggest the possible superiority of MMC-coated charcoal over free MMC both because of a lessening of the effects on host immunity due to prolonged slow drug release and because of the increase in cytolytic macrophages that was induced.
Sakita
M
M
Department of Surgery, Meiji College of Oriental Medicine, Kyoto-fu.
Iwamoto
A
A
Hata
K
K
Hagiwara
A
A
Takahashi
T
T
D000327:Adsorption; D000818:Animals; D002244:Carbon / Q000008:administration & dosage; D007274:Injections, Intraperitoneal; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D016685:Mitomycin; D008937:Mitomycins / Q000008:administration & dosage / Q000494:pharmacology*; D009929:Organ Size; D010529:Peritoneal Cavity / Q000166:cytology*; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000033:anatomy & histology; D013936:Thymidine / Q000378:metabolism; D013950:Thymus Gland / Q000033:anatomy & histology; D013997:Time Factors
D008937:Mitomycins; D016685:Mitomycin; D002244:Carbon; D013936:Thymidine