Cloning and expression of a human muscle phosphofructokinase cDNA.
The nucleotide sequence of a 2.86-kb cDNA clone containing the complete human muscle phosphofructokinase (PFK) protein-coding region was determined. It comprises 76 bp of 5'-untranslated sequence, 2340 bp encoding human muscle PFK polypeptide, and 399 bp of 3'-untranslated sequence plus a poly(A) tract. A retroviral vector was utilized to express the product of this coding sequence in mouse fibroblasts. The PFK-coding cDNA was shown to code for an enzymatically active polypeptide by immunoprecipitation analysis and DEAE-Sephadex A-25 chromatography.
Sharma
P M
PM
Department of Molecular and Experimental Medicine, Scripps Clinic and Research Foundation, La Jolla, CA 92037.
Reddy
G R
GR
Vora
S
S
Babior
B M
BM
McLachlan
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002852:Chromatography, Ion Exchange; D003001:Cloning, Molecular; D004247:DNA / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D005796:Genes; D005822:Genetic Vectors; D006801:Humans; D008969:Molecular Sequence Data; D009132:Muscles / Q000201:enzymology*; D010732:Phosphofructokinase-1 / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D011233:Precipitin Tests; D012190:Retroviridae / Q000235:genetics; D014162:Transfection
D004247:DNA; D010732:Phosphofructokinase-1