title: Cytochemical localization of plasma membrane enzyme markers during interiorization of tachyzoites of Toxoplasma gondii by macrophages.
abstract: The enzyme activity of Mg++-ATPase, Na+-K+-ATPase, 5'-nucleotidase and NAD(P)H-oxidase was cytochemically detected at the ultrastructural level in mouse peritoneal macrophages infected with untreated and with specific antibody-coated Toxoplasma gondii tachyzoites. The Mg++-ATPase and 5'-nucleotidase were distributed throughout the macrophages' plasma membrane but were not observed in the membrane lining endocytic vacuoles containing ingested parasites; however, Na+-K+-ATPase activity was detected in the macrophages' plasma membrane as well as in the parasitophorous vacuoles that contained untreated or specific antibody-coated parasites. Reaction product, indicative of NAD(P)H-oxidase, was detected in the parasitophorous vacuoles that contained only specific antibody-coated parasites.
authors.affiliation: Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brasil.
authors.forename: L
authors.initials: L
authors.lastname: de Carvalho
authors.forename: W
authors.initials: W
authors.lastname: de Souza
mesh_terms: D015720:5'-Nucleotidase; D000251:Adenosine Triphosphatases / Q000032:analysis*; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000032:analysis; D002462:Cell Membrane / Q000201:enzymology; D006651:Histocytochemistry; D008264:Macrophages / Q000201:enzymology / Q000469:parasitology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D009247:NADH, NADPH Oxidoreductases / Q000032:analysis*; D019255:NADPH Oxidases; D009708:Nucleotidases / Q000032:analysis*; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis; D014122:Toxoplasma / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D009247:NADH, NADPH Oxidoreductases; D019255:NADPH Oxidases; D009708:Nucleotidases; D015720:5'-Nucleotidase; D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase