title: [Tissue culture and estrogen, to clarify the roles of estrone sulfate].
abstract: Estrone sulfate (E1-S) has been shown to be quantitatively the most important estrogen in peripheral blood. But, the physiological and/or pathological role of E1-S is not yet clarified. At present, we tried to clarify it using tissue cultures. In tissue cultures of human endometrium, secretory endometrium showed higher activity of estrone sulfatase (E1----E1-S) than proliferative endometrium. Progesterone added in the medium induced an increase of estrone sulfotransferase in the proliferative endometrium. The results suggest a reducing effect of estrogen by progesterone in secretory endometrium in physiological conditions. Estrogen dependent malignant tumors (breast cancer, endometrial cancer) have high estrone sulfatase. It converts E1-S to E1 (----E2) which are abundant in these tumors. Ishikawa cell line increased estrone sulfotransferase activity with progesterone, somewhat like the physiological conditions. From out study in vivo, there is a possibility of some ameliorative effects of E1-S on the central nervous system of patients with senile dementia (Alzheimer's type). Effects of E1-S on central nerves were investigated using tissue cultures.
authors.affiliation: Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Honjo
authors.forename: M
authors.initials: M
authors.lastname: Urabe
authors.forename: K
authors.initials: K
authors.lastname: Naitoh
authors.forename: Y
authors.initials: Y
authors.lastname: Ogino
authors.forename: J
authors.initials: J
authors.lastname: Kitawaki
authors.forename: J
authors.initials: J
authors.lastname: Yasuda
authors.forename: T
authors.initials: T
authors.lastname: Yamamoto
authors.forename: H
authors.initials: H
authors.lastname: Okada
mesh_terms: D000544:Alzheimer Disease / Q000188:drug therapy; D001943:Breast Neoplasms / Q000378:metabolism; D046508:Culture Techniques; D016889:Endometrial Neoplasms / Q000378:metabolism; D004717:Endometrium / Q000378:metabolism*; D004960:Estradiol Dehydrogenases / Q000378:metabolism; D004970:Estrone / Q000031:analogs & derivatives* / Q000378:metabolism / Q000502:physiology / Q000627:therapeutic use; D005260:Female; D006801:Humans; D011374:Progesterone / Q000502:physiology; D013429:Sulfatases / Q000378:metabolism; D015238:Sulfotransferases; D013466:Sulfurtransferases / Q000378:metabolism
chemical_list: D004970:Estrone; D011374:Progesterone; D004960:Estradiol Dehydrogenases; D013466:Sulfurtransferases; D015238:Sulfotransferases; C020122:estrone sulfotransferase; D013429:Sulfatases; C020121:estrone sulfatase; C017296:estrone sulfate