title: [Utilization of free and protein-bound lysine in the Japanese quail].
abstract: The utilization of dietary lysine for protein synthesis is affected by the digestibility of protein-bound lysine, by its intestinal resorption and by its oxidative catabolism. The approach chosen in this paper enables a comparison of the cumulative effect of these processes on the utilization of free and protein-bound lysine, respectively. The principle of the approach is based on a quantification of the expiration of 14C-labelled carbon dioxide after an oral administration of a diet, which contains L-(U-14C)-lysine either as a free amino acid or bound to yeast proteins. During an adaptation phase cockerels of the Japanese quail received a diet based mainly on ground wheat and wheat gluten. This diet was supplemented either with yeast proteins or with a mixture of L-amino acids which simulates the composition of the yeast proteins. In the main experiment the expiration of labelled carbon dioxide was measured during 240 minutes after the administration of the corresponding labelled diets. Just before treatment the animals were either in the postprandial phase or in a state of slight hunger. The maximum of expiration of labelled carbon dioxide occurred around the 60th minute after administration of the corresponding labelled diets. The cumulative expiration of labelled carbon dioxide, expressed in per cent of the radioactive dose used, amounts to 15.5% and 14.3% for free and protein-bound lysine, respectively. The utilization of both forms of lysine in the Japanese quail is lower than in broilers.
authors.forename: M
authors.initials: M
authors.lastname: Otto
authors.forename: M
authors.initials: M
authors.lastname: Snejdárková
mesh_terms: D000821:Animal Feed; D000818:Animals; D003370:Coturnix / Q000378:metabolism*; D008239:Lysine / Q000378:metabolism*; D008297:Male; D011485:Protein Binding; D011784:Quail / Q000378:metabolism*
chemical_list: D008239:Lysine
vernacular_title: Vyuzitie vol'ného a v bielkovinách viazaného lyzínu u prepelice japonskej.