cDNA cloning and sequencing of phospholamban from pig stomach smooth muscle.
Phospholamban cDNA from pig stomach smooth muscle was cloned and sequenced. The 737-nucleotide-residue cDNA contained an open reading frame of 156 nucleotide residues encoding a peptide of 52 amino acid residues (Mr 6080). This peptide shares 100% sequence identity with dog cardiac-muscle phospholamban. It differs from rabbit cardiac-muscle and slow-twitch skeletal-muscle phospholamban only at position 2, which is a glutamic acid residue in rabbit phospholamban, but an aspartic acid residue in the pig smooth-muscle protein. Northern-blot analysis reveals the presence of several phospholamban mRNAs in smooth muscle, but a 900-nucleotide-residue and a 2800-nucleotide-residue transcript predominate.
Verboomen
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H
Laboratorium voor Fysiologie, Katholieke Universiteit Leuven, Campus Gasthuisberg, Belgium.
Wuytack
F
F
Eggermont
J A
JA
De Jaegere
S
S
Missiaen
L
L
Raeymaekers
L
L
Casteels
R
R
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002135:Calcium-Binding Proteins; D003001:Cloning, Molecular; D015342:DNA Probes; D004270:DNA, Circular; D008969:Molecular Sequence Data; D009130:Muscle, Smooth / Q000201:enzymology*; D013552:Swine; D000099211:Phospholamban
D000251:Adenosine Triphosphatases; D002135:Calcium-Binding Proteins; D015342:DNA Probes; D004270:DNA, Circular; D000099211:Phospholamban