Production of interferon-gamma during infection of mice with Plasmodium chabaudi chabaudi.
An ELISA assay, designed to detect interferon-gamma (IFN-gamma) in the picogram range, was used to study the presence of IFN-gamma in serum and its production by T cells taken from C57BL/6 mice infected with Plasmodium chabaudi chabaudi. IFN-gamma was detectable in mouse plasma for two to three days before the peak of parasitaemia. Similarly, IFN-gamma production by T cells could be detected in vitro. In limiting dilution cultures, the production of IFN-gamma by as few as 1,000 T cells was detectable using this assay. The limiting dilution analysis revealed that a substantial IFN-gamma response by specific T cells occurs very early in a primary infection with P. chabaudi.
Slade
S J
SJ
Max-Planck-Institut für Immunobiologie, Freiburg, Federal Republic of Germany.
Langhorne
J
J
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000032:analysis / Q000096:biosynthesis* / Q000097:blood; D008213:Lymphocyte Activation; D008288:Malaria / Q000097:blood / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000276:immunology
D007371:Interferon-gamma