Depletion of PDGF from serum inhibits growth of AIDS-related and sporadic Kaposi's sarcoma cells in culture.
We have examined the mitogenic potential of platelet-derived growth factor (PDGF) on AIDS (acquired immune deficiency syndrome)-related and sporadic Kaposi's sarcoma cells in comparison to fibroblasts at physiological and subphysiological calcium concentrations of the culture medium. At low calcium concentrations in the presence of 3% human serum the growth rate of fibroblasts and Kaposi's sarcoma (KS) cells is similarly reduced to less than half of the growth rate at physiological Ca2+ concentrations. In the presence of 3% PDGF depleted, platelet poor plasma-derived serum (PPPS) growth of KS cells ceased completely, whereas fibroblasts made 1-2 cell divisions within 15 days. At physiological Ca2+ concentrations, the reduced PDGF content in 3% PPPS had no effect on human embryonal fibroblasts and little effect on adult skin fibroblasts. In contrast, KS cells became growth-arrested after one to two doublings. This is consistent with the observation that PDGF B-chain mRNA could not be detected in our KS cells whereas PDGF receptor mRNA was expressed.
Roth
W K
WK
Max-Planck-Institut für Biochemie, Martinsried, Federal Republic of Germany.
Werner
S
S
Schirren
C G
CG
Hofschneider
P H
PH
D000163:Acquired Immunodeficiency Syndrome / Q000473:pathology*; D002118:Calcium / Q000494:pharmacology; D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media; D006801:Humans; D010982:Platelet-Derived Growth Factor / Q000032:analysis / Q000502:physiology*; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000032:analysis; D011956:Receptors, Cell Surface / Q000235:genetics; D017479:Receptors, Platelet-Derived Growth Factor; D012514:Sarcoma, Kaposi / Q000473:pathology*
D003470:Culture Media; D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D017479:Receptors, Platelet-Derived Growth Factor; D002118:Calcium