title: Irreversible binding of bacteriophage T5 to its FhuA receptor protein is associated with covalent cross-linking of 3 copies of tail protein pb4.
abstract: Irreversible binding of bacteriophage T5 to its FhuA receptor protein is characterized by a high activation energy, typical for reactions where covalent bonds are formed [Zarnitz, M.L. and Weidel, W. (1963) Z. Naturforsch. 18b, 276-280]. Upon binding of radiolabeled T5 phages to FhuA formation of a new protein of 250 kDa was observed. Using electrophoretical and Western blotting techniques this protein was shown to be formed by cross-linking of 3 copies of tail protein pb4, rather than by cross-linking of FhuA and the receptor-binding protein.
authors.affiliation: Universität Konstanz, Fakultät für Biologie, Konstanz, FRG.
authors.forename: A
authors.initials: A
authors.lastname: Feucht
authors.forename: G
authors.initials: G
authors.lastname: Heinzelmann
authors.forename: K J
authors.initials: KJ
authors.lastname: Heller
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins; D008970:Molecular Weight; D011485:Protein Binding; D011991:Receptors, Virus; D013604:T-Phages / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*; D017376:Viral Tail Proteins
chemical_list: D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; C050900:FhuA protein, E coli; D011991:Receptors, Virus; D014764:Viral Proteins; D017376:Viral Tail Proteins