Inhibition of growth of Trichophyton rubrum by the myeloperoxidase-hydrogen peroxide-chloride system.
The myeloperoxidase-hydrogen peroxide-chloride (MPO-H2O2-Cl) system is an antimicrobial system of polymorphonuclear leukocytes. We demonstrated that the MPO-H2O2-Cl system is fungicidal for Trichophyton rubrum. Fungal growth of a synchronous cell culture of T. rubrum germlings was assayed by measuring the uptake of tritiated N-acetyl-D-glucosamine, and the viability of the fungi was assayed by counting colony-forming units. Cytotoxins produced by the interaction of myeloperoxidase with hydrogen peroxide and chloride ion were fungicidal for T. rubrum. Growth inhibition was abolished in the presence of catalase or L-methionine. Polymorphonuclear leukocytes through the MPO-H2O2-Cl system may prevent invasion and sepsis by dermatophytes even in the absence of specific immunity.
MacCarthy
K G
KG
Department of Dermatology, University of Minnesota Medical School, Minneapolis.
Dahl
M V
MV
D000117:Acetylglucosamine / Q000378:metabolism; D002374:Catalase / Q000494:pharmacology; D002712:Chlorides / Q000502:physiology*; D015169:Colony Count, Microbial; D006861:Hydrogen Peroxide / Q000494:pharmacology / Q000502:physiology*; D008715:Methionine / Q000494:pharmacology; D009195:Peroxidase / Q000502:physiology*; D014249:Trichophyton / Q000254:growth & development* / Q000378:metabolism
D002712:Chlorides; D008715:Methionine; D006861:Hydrogen Peroxide; D002374:Catalase; D009195:Peroxidase; D000117:Acetylglucosamine