Characterization of RNA transcripts from herpes simplex virus-1 DNA fragment BamHI-B.
Herpes simplex virus type 1 (HSV-1) DNA fragment BamHI-B (0.738-0.809 map units) was recently reported to be associated with the phenotype of intraperitoneal pathogenicity and to encode a latency-associated RNA transcript. Part of this fragment resides within the internal repeat sequence of the long (L) region of the viral genome. In this study, RNA transcripts from BamHI-B were characterized. In addition to immediate-early mRNAs IE-1 and IE-2, eight novel RNA species were found. Three transcripts were mapped in the repeat regions of this fragment and five transcripts in the unique L region of BamHI-B. In addition, transcription activity from these regions was compared in several HSV-1 strains. These included the intraperitoneal virulent F and KOS strains, the avirulent strain HFEM, as well as the HFEM/F intratypic virulent recombinant R-MIC1. Several differences were noted and their possible relevance to virulence is discussed.
Ben-Hur
T
T
Department of Molecular Virology, Faculty of Medicine, Hebrew University, Jerusalem, Israel.
Moyal
M
M
Rösen-Wolff
A
A
Darai
G
G
Becker
Y
Y
D000818:Animals; D015152:Blotting, Northern; D015342:DNA Probes; D004279:DNA, Viral / Q000235:genetics*; D015245:Deoxyribonuclease BamHI; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000235:genetics*; D015183:Restriction Mapping; D018139:Simplexvirus / Q000235:genetics*; D014158:Transcription, Genetic
D015342:DNA Probes; D004279:DNA, Viral; D012367:RNA, Viral; D015245:Deoxyribonuclease BamHI