Effects of peripheral benzodiazepines upon the O2 consumption of neuroblastoma cells.
The effects of peripheral benzodiazepines on the respiration of a neuronal cell, the mouse C 1300 neuroblastoma, were analyzed. The presence of 'peripheral receptors' to the [3H]PK 11195 ligand was checked in these cells. A dose-dependent decrease of the O2 consumption in the presence of Ro 5-4864 and PK 11195 was observed at concentrations consistent with a receptor-mediated action. Diazepam, clonazepam and Ro 15-1788 were inactive. Previous studies have localized the peripheral benzodiazepine receptors on the mitochondrial outer membrane. We report here an effect of the peripheral ligand on mitochondrial metabolism.
Larcher
J C
JC
Laboratoire de Biochimie Cellulaire, Collège de France, Paris, France.
Vayssiere
J L
JL
Le Marquer
F J
FJ
Cordeau
L R
LR
Keane
P E
PE
Bachy
A
A
Gros
F
F
Croizat
B P
BP
D000818:Animals; D001569:Benzodiazepines / Q000494:pharmacology*; D002478:Cells, Cultured; D002998:Clonazepam / Q000494:pharmacology; D002999:Clone Cells; D003975:Diazepam / Q000494:pharmacology; D005442:Flumazenil / Q000378:metabolism; D007546:Isoquinolines / Q000378:metabolism; D051379:Mice; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D009447:Neuroblastoma / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects*; D011963:Receptors, GABA-A / Q000187:drug effects / Q000378:metabolism
D007546:Isoquinolines; D011963:Receptors, GABA-A; D001569:Benzodiazepines; D005442:Flumazenil; D002998:Clonazepam; D003975:Diazepam; C037850:PK 11195