Truncated forms of human complement factor H.
By the use of Western-blot analyses with polyclonal anti-(Factor H) antibodies, two low-Mr protein species of Mr 41,000 and 37,000 under non-reducing conditions and 43,000 and 40,000 under reducing conditions are consistently detected together with the well-known 155,000-Mr Factor H in human plasma and serum. These two additional species are also found in plasma, urine and synovial fluids. The 41,000-Mr species but not the 37,00-Mr species is detected by a monoclonal anti-(Factor H) antibody directed at the N-terminal part of Factor H. The 37,000-Mr species but not the 41,000-Mr species is detected by a monoclonal anti-(Factor H) antibody directed at the C-terminal part of Factor H. The 41,000-Mr and 37,000-Mr species are different from the well-characterized 36,000-Mr N-terminal tryptic fragment of Factor H. They are likely to represent translational products of the short Factor H mRNA species of 1.8 kb and 1.2-1.5 kb occurring in human liver that we have recently described.
Fontaine
M
M
I.N.S.E.R.M. Unité 78, Bois-Guillaume, France.
Demares
M J
MJ
Koistinen
V
V
Day
A J
AJ
Davrinche
C
C
Sim
R B
RB
Ripoche
J
J
D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D003180:Complement C3b Inactivator Proteins / Q000032:analysis*; D017242:Complement Factor H; D006801:Humans; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis
D000911:Antibodies, Monoclonal; C493495:CFH protein, human; D003180:Complement C3b Inactivator Proteins; D010446:Peptide Fragments; D017242:Complement Factor H