Two different virus-inducible elements are required for human beta-interferon gene regulation.
We show that the human beta-interferon gene promoter contains two different virus-inducible regulatory elements, PRDI and PRDII (positive regulatory domains I and II). A single copy of either element alone has no discernible transcriptional activity in mouse fibroblasts. However, multiple copies of either element function as a constitutive or virus-inducible transcription element depending on the cell line in which the sequence was tested. These results in conjunction with previous studies suggest that virus induction of the human beta-interferon gene is achieved through cooperative interactions between two entirely distinct virus-inducible elements. Comparison of the properties of these elements reveals that multiple copies of PRDI, but not PRDII, can be activated by three different inducers, beta-IFN, gamma-IFN and virus. These results suggest that the pathways of virus and interferon induction may share at least one common regulatory component.
Fan
C M
CM
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138.
Maniatis
T
T
D000818:Animals; D001483:Base Sequence; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation / Q000187:drug effects; D005814:Genes, Viral; D006801:Humans; D007370:Interferon Type I / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology; D007739:L Cells; D051379:Mice; D008969:Molecular Sequence Data; D010222:Parainfluenza Virus 1, Human / Q000502:physiology; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D013539:Simian virus 40 / Q000235:genetics; D013045:Species Specificity
D007370:Interferon Type I; D007371:Interferon-gamma