Transforming growth factors beta 1 and beta 2 as well as milk growth factor decrease anti-CD3-induced proliferation of human lymphocytes without inhibiting the anti-CD3-mediated increase of [Ca2+]i and the activation of protein kinase C.
Porcine transforming growth factor 1 and 2 (pTGF-beta 1 and -beta 2) and milk growth factor (MGF) at 1 ng/ml significantly inhibited the proliferation of human lymphocytes induced by anti-CD3 antibodies. In contrast, the anti-CD3-mediated increase of intracellular Ca2+ and the activation and translocation of protein kinase C were not affected by the transforming growth factors.
Stoeck
M
M
Ludwig Institute for Cancer Research, Epalinges, Switzerland.
Sommermeyer
H
H
Miescher
S
S
Cox
D
D
Alkan
S
S
Szamel
M
M
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D001685:Biological Factors / Q000494:pharmacology*; D017252:CD3 Complex; D002118:Calcium / Q000378:metabolism*; D004789:Enzyme Activation; D006801:Humans; D011493:Protein Kinase C / Q000378:metabolism*; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D013552:Swine; D013601:T-Lymphocytes / Q000276:immunology*; D015291:Transforming Growth Factors / Q000494:pharmacology*
D000945:Antigens, Differentiation, T-Lymphocyte; D001685:Biological Factors; D017252:CD3 Complex; D011948:Receptors, Antigen, T-Cell; D015291:Transforming Growth Factors; D011493:Protein Kinase C; D002118:Calcium