Decreased atrial natriuretic factor receptors and impaired cGMP generation in glomeruli from the cardiomyopathic hamster.
To determine a possible basis for the decreased action of atrial natriuretic factors (ANF) in congestive heart failure, we compared the cardiomyopathic hamster (CMH) in frank congestive failure, and the age-matched, normal, F1B strain of Golden Syrian Hamsters. Scatchard analysis of competitive binding studies revealed two classes of glomerular receptors. The CMH exhibited decreased binding overall and a markedly decreased number of high affinity receptors but comparable receptor affinity compared to the F1B. In contrast, the low affinity receptor population in the CMH had a much greater affinity compared to the F1B while receptor number was similar. Plasma ANF levels were substantially elevated in the CMH compared to the F1B and in-vitro generation of cGMP was significantly lower in the CMH. Such abnormalities could contribute to the resistance to ANF in this disease.
Levin
E R
ER
Long Beach Veterans Hospital, Irvine, CA 90822.
Frank
H J
HJ
Chaudhari
A
A
Kirschenbaum
M A
MA
Bandt
A
A
Mills
S
S
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism*; D009202:Cardiomyopathies / Q000378:metabolism*; D006224:Cricetinae; D006152:Cyclic GMP / Q000096:biosynthesis*; D006333:Heart Failure / Q000378:metabolism; D007678:Kidney Glomerulus / Q000378:metabolism*; D007700:Kinetics; D008647:Mesocricetus; D017461:Receptors, Atrial Natriuretic Factor; D011956:Receptors, Cell Surface / Q000378:metabolism*; D015290:Second Messenger Systems
D011956:Receptors, Cell Surface; D009320:Atrial Natriuretic Factor; D017461:Receptors, Atrial Natriuretic Factor; D006152:Cyclic GMP