Establishment of hamster pancreatic ductal carcinoma cell line (PC-1) producing blood group-related antigens.
A pancreatic cancer cell line, PC-1, was established from a pancreatic ductal carcinoma induced in a hamster by N-nitrosobis(2-oxopropyl)amine (BOP). The cells grew in a monolayer with a doubling time of 38 h, and floated or piled up to form a duct-like structure. Chromosome counts ranged from 42 to 89. Light and electron microscopic studies of PC-1 cells revealed production of conspicuous amounts of amorphous substance. Injection of PC-1 cells into the homologous hamster pancreas resulted in tumor formation, histopathologically indistinguishable from the original primary pancreatic ductal carcinoma. Immunohistochemical expression of blood group-related antigens (BGRAs), A, B, H, Leb, Lex and Ley was observed both in the cells in the culture, and in tumor transplanted into the pancreas. In the culture supernatant, a high titer of blood group A antigen was detected. This cell line may provide a unique tool for studying the mechanism of BGRA synthesis and release in malignant cells.
Egami
H
H
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha 68105.
Takiyama
Y
Y
Cano
M
M
Houser
W H
WH
Pour
P M
PM
D000818:Animals; D001789:Blood Group Antigens; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000276:immunology / Q000473:pathology* / Q000648:ultrastructure; D002455:Cell Division; D002460:Cell Line; D006224:Cricetinae; D046508:Culture Techniques / Q000379:methods; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D009368:Neoplasm Transplantation; D010190:Pancreatic Neoplasms / Q000276:immunology / Q000473:pathology* / Q000648:ultrastructure
D001789:Blood Group Antigens