title: Primary structure of a mouse mastocytoma proteoglycan core protein.
abstract: The complete nucleotide sequence of a mouse mastocytoma proteoglycan core protein mRNA was determined. The mRNA, estimated to contain 1.1 kb, encodes a protein with an Mr of 16715. A 21-amino acid-residue region of the protein is composed of alternating serine and glycine residues. Southern-blot analysis of mouse genomic DNA with cDNA containing sequences corresponding to the Ser-Gly repeat region revealed more than 15 gene fragments. Hybridization with a probe corresponding to the N-terminal portion of the core protein identified two fragments, and cDNA covering the C-terminal part of the core protein and the 3' untranslated part of the mRNA hybridized to a single fragment. Antibodies against the core protein, obtained after immunization of rabbits with a fusion protein, reacted with both chondroitin sulphate proteoglycans and heparin proteoglycans produced by the tumour. In immunoblotting of a microsomal fraction from the mastocytoma, the antiserum recognized a single protein (Mr 17,000), which probably represents the core protein before glycosylation.
authors.affiliation: Department of Veterinary Medical Chemistry, Swedish University of Agricultural Sciences, Uppsala.
authors.forename: L
authors.initials: L
authors.lastname: Kjellén
authors.forename: I
authors.initials: I
authors.lastname: Pettersson
authors.forename: P
authors.initials: P
authors.lastname: Lillhager
authors.forename: M L
authors.initials: ML
authors.lastname: Steen
authors.forename: U
authors.initials: U
authors.lastname: Pettersson
authors.forename: P
authors.initials: P
authors.lastname: Lehtonen
authors.forename: T
authors.initials: T
authors.lastname: Karlsson
authors.forename: E
authors.initials: E
authors.lastname: Ruoslahti
authors.forename: L
authors.initials: L
authors.lastname: Hellman
mesh_terms: D053674:Aggrecans; D000595:Amino Acid Sequence; D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins / Q000235:genetics*; D037181:Lectins, C-Type; D012515:Mast-Cell Sarcoma / Q000235:genetics*; D051379:Mice; D008861:Microsomes / Q000378:metabolism; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D011487:Protein Conformation; D011509:Proteoglycans; D012333:RNA, Messenger / Q000235:genetics*
chemical_list: C508320:Acan protein, mouse; D053674:Aggrecans; D015342:DNA Probes; D004273:DNA, Neoplasm; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D037181:Lectins, C-Type; D009363:Neoplasm Proteins; D011509:Proteoglycans; D012333:RNA, Messenger; D004247:DNA