title: Isolation and characterization of a mammalian gene encoding a high-affinity cAMP phosphodiesterase.
abstract: A rat brain cDNA library has been constructed in a Saccharomyces cerevisiae expression vector and used to isolate genes that can function in yeast to suppress the phenotypic effects of RAS2val19, a mutant form of the RAS2 gene analogous to an oncogenic mutant of the human HRAS gene. One cDNA, DPD, was cloned and its genetic and biochemical properties were characterized. A DPD product would share 80% amino acid sequence identity with the Drosophila melanogaster dunce-encoded protein over an extended region. We have shown that the DPD protein is a high-affinity cAMP-specific phosphodiesterase.
authors.affiliation: Cold Spring Harbor Laboratory, NY 11724.
authors.forename: J
authors.initials: J
authors.lastname: Colicelli
authors.forename: C
authors.initials: C
authors.lastname: Birchmeier
authors.forename: T
authors.initials: T
authors.lastname: Michaeli
authors.forename: K
authors.initials: K
authors.lastname: O'Neill
authors.forename: M
authors.initials: M
authors.lastname: Riggs
authors.forename: M
authors.initials: M
authors.lastname: Wigler
mesh_terms: D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D005816:Genetic Complementation Test; D006358:Hot Temperature; D008969:Molecular Sequence Data; D051381:Rats; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics
chemical_list: D004247:DNA; D015105:3',5'-Cyclic-AMP Phosphodiesterases