Detection of human and marmoset immunoglobulin heavy chain by a polyclonal antiserum to a marmoset immunoglobulin-related T cell product.
We show that a polyclonal rabbit antiserum raised against a purified monoclonal T cell component from a lower primate (cotton-topped marmoset) reacts by immunoblot transfer (Western Blot analysis) with serum immunoglobulin of man and marmoset. The antigenic component had an approximate mass of 68 kilodaltons and was isolated by immune-affinity chromatography from culture fluid in which the marmoset T cell leukemia 70-N2 had been grown. The reaction with human serum immunoglobulin is with a subset of the IgG molecules and is localized to the gamma heavy chain. The reaction with marmoset serum immunoglobulin is predominantly directed against the heavy chain, but slight reactivity is also noted against the light chains. These results substantiate reports of serological cross-reactions between immunoglobulin-like T cell receptors and classical immunoglobulins and illustrate the similarity between immunoglobulins of man and those of a distantly related New World primate.
Speidel
M T
MT
Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston.
Marchalonis
J J
JJ
D000818:Animals; D000918:Antibody Specificity; D000951:Antigens, Neoplasm / Q000276:immunology / Q000302:isolation & purification; D000954:Antigens, Surface / Q000276:immunology; D003429:Cross Reactions; D006801:Humans; D007106:Immune Sera; D007143:Immunoglobulin Heavy Chains / Q000276:immunology*; D007942:Leukemia, Experimental / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D012452:Saguinus / Q000276:immunology*; D013601:T-Lymphocytes / Q000737:chemistry / Q000276:immunology*; D014407:Tumor Cells, Cultured / Q000276:immunology
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D007106:Immune Sera; D007143:Immunoglobulin Heavy Chains; D011948:Receptors, Antigen, T-Cell