Involvement of immunoglobulin heavy- and light-chain (kappa) gene clusters in a human B-cell translocation, t(2;14).
The breakpoints of a translocation, t(2;14)(p11;q32), detected in an Epstein-Barr virus-transformed lymphoid B-cell line were mapped by Southern analysis, field-inversion gel electrophoresis, and in situ hybridisation. The translocation involved the immunoglobulin light-chain (kappa) locus on chromosome 2 and the heavy-chain locus on chromosome 14. The breakpoint on chromosome 2 was between VK and CK, most likely within JK. The chromosome 14 break was located within the VH cluster, no more than 220 kb 5' of the productively rearranged JH locus. The translocation probably resulted from an aberrant rearrangement of the kappa light-chain genes.
Kennedy
M A
MA
Cytogenetic and Molecular Oncology Unit, Christchurch Hospital, New Zealand.
Morris
C M
CM
Hollings
P E
PE
Fitzgerald
P H
PH
D001402:B-Lymphocytes / Q000648:ultrastructure*; D015139:Blotting, Southern; D002883:Chromosomes, Human, Pair 14; D002889:Chromosomes, Human, Pair 2; D015342:DNA Probes; D004587:Electrophoresis, Agar Gel; D005260:Female; D005803:Genes, Immunoglobulin; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007133:Immunoglobulin Joining Region / Q000235:genetics; D007147:Immunoglobulin Light Chains / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics; D005810:Multigene Family; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
D015342:DNA Probes; D007143:Immunoglobulin Heavy Chains; D007133:Immunoglobulin Joining Region; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region