Crystallization and preliminary X-ray studies of pyridoxal kinase from sheep brain.
Crystals of pyridoxal kinase from sheep brain have been grown from solutions of ammonium sulfate or Na+/K+ phosphate. The crystals are trigonal, space group P3(1)21 with axes a = b = 102.2 A and c = 58.5 A. The crystals are quite stable to x-rays and diffract at 2.2-A resolution. This macromolecule is a 80,000-kDa dimer and utilizes the 2-fold symmetry of the space group, resulting in one subunit/asymmetric unit.
Arnone
A
A
Department of Biochemistry, University of Iowa, Iowa City 52242.
Rogers
P
P
Scholz
G
G
Kwok
F
F
D000645:Ammonium Sulfate; D000818:Animals; D001921:Brain / Q000201:enzymology*; D003460:Crystallization; D046911:Macromolecular Substances; D008970:Molecular Weight; D010710:Phosphates; D010770:Phosphotransferases; D011188:Potassium; D017680:Potassium Compounds; D011731:Pyridoxal Kinase; D012756:Sheep; D012996:Solutions; D014961:X-Ray Diffraction
D046911:Macromolecular Substances; D010710:Phosphates; D017680:Potassium Compounds; D012996:Solutions; C013216:potassium phosphate; D010770:Phosphotransferases; D011731:Pyridoxal Kinase; D011188:Potassium; C018279:sodium phosphate; D000645:Ammonium Sulfate