Expression and characterization of a protein encoded by the human c-myc exon 1 in Escherichia coli.
Our previously reported data from DNA sequence studies of the c-myc locus show that the human c-myc exon 1 has an open reading frame capable of encoding a protein of 188 amino acid residues. To confirm the presence of the open reading frame, we constructed a recombinant vector (pMCP60) that contains a segment of the lambda cII translational initiation region, a portion of the N-terminus of the v-mos gene, and 639 base pairs of the first exon of the human c-myc gene. pMCP60 expresses a 38 kilodalton tripartate protein (cII-mos-myc), which was purified by high-pressure liquid chromatography. The presence of myc exon 1 sequences in the cII-mos-myc fusion protein was confirmed by partial amino acid sequence analysis. These experiments further establish that the first exon of the human c-myc gene contains an open reading frame capable of expressing a protein in Escherichia coli.
Psallidopoulos
M C
MC
Laboratory of Molecular Oncology, National Cancer Institute, Frederick, Maryland 21701-1013.
Seth
A
A
Dubois
G C
GC
Fisher
R J
RJ
Papas
T S
TS
D000595:Amino Acid Sequence; D001483:Base Sequence; D015153:Blotting, Western; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D003062:Codon; D004926:Escherichia coli / Q000235:genetics*; D005091:Exons; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D017231:Proto-Oncogene Proteins c-mos; D016271:Proto-Oncogene Proteins c-myc; D011519:Proto-Oncogenes; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification
D003062:Codon; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D011993:Recombinant Fusion Proteins; D017231:Proto-Oncogene Proteins c-mos