The tumor cells (FA-6) established from a pancreatic cancer associated with humoral hypercalcemia of malignancy: a simultaneous production of parathyroid hormone-like activity and transforming growth factor activity.
Human pancreatic cancer cells (FA-6) producing bone resorbing factor were established in culture. A biopsied lymphnode from a patient with pancreatic cancer associated with humoral hypercalcemia of malignancy (HHM) was transplanted to nude mice, and the cells producing high parathyroid hormone (PTH)-like activity were selected by a limited dilution from outgrowth of the xenografts of the tumor grown in nude mice. The conditioned media contained an activity to stimulate the resorption of mouse calvaria in vitro which was indomethacin-insensitive. The conditioned media had both alpha-type and beta-type transforming growth factor (TGF) activity but no interleukin-1 activity. TGF-alpha activity was co-eluted with PTH-like activity from gel-chromatography at around 15 kDa. The FA-6 cells now established are the first cells of pancreatic cancer associated with HHM producing both PTH-like and TGF-alpha activities along with bone resorbing activity.
Nagata
N
N
Third Department of Internal Medicine, National Defense Medical College, Saitama, Japan.
Akatsu
T
T
Kugai
N
N
Yasutomo
Y
Y
Kinoshita
T
T
Kosano
H
H
Shimauchi
T
T
Takatani
O
O
Ueyama
Y
Y
D000818:Animals; D001681:Biological Assay; D001862:Bone Resorption / Q000187:drug effects; D001842:Bone and Bones / Q000187:drug effects / Q000378:metabolism; D002850:Chromatography, Gel; D003470:Culture Media; D000242:Cyclic AMP / Q000096:biosynthesis; D006801:Humans; D006934:Hypercalcemia / Q000209:etiology*; D007213:Indomethacin / Q000494:pharmacology; D007621:Karyotyping; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010190:Pancreatic Neoplasms / Q000150:complications / Q000235:genetics / Q000378:metabolism*; D010281:Parathyroid Hormone / Q000096:biosynthesis* / Q000494:pharmacology; D051381:Rats; D015291:Transforming Growth Factors / Q000096:biosynthesis* / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D003470:Culture Media; D010281:Parathyroid Hormone; D015291:Transforming Growth Factors; D000242:Cyclic AMP; D007213:Indomethacin