Regulation of luminescence by cyclic AMP in cya-like and crp-like mutants of Vibrio fischeri.
Mutants of Vibrio fischeri MJ-1 (wild type) apparently deficient in adenylate cyclase (cya-like) or cyclic AMP receptor protein (crp-like) were isolated and characterized. Compared with MJ-1, the mutants produced low levels of luminescence and luciferase. Addition of cyclic AMP restored wild-type levels of luminescence and luciferase in the cya-like mutant but not in the crp-like mutant. The results are consistent with the hypothesis that in V. fischeri cyclic AMP and cyclic AMP receptor protein are required for induction of the luminescence system.
Dunlap
P V
PV
Department of Biology, New Mexico State University, Las Cruces 88003.
D000242:Cyclic AMP / Q000494:pharmacology*; D005578:Fosfomycin / Q000494:pharmacology; D007700:Kinetics; D008163:Luminescent Measurements; D009154:Mutation; D013307:Streptomycin / Q000494:pharmacology; D014733:Vibrio / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*
D005578:Fosfomycin; D000242:Cyclic AMP; D013307:Streptomycin