The response of the rat urinary bladder microcirculation to photodynamic therapy.
Light activation of dihematoporphyrin ether (photofrin II) has been used in the treatment of bladder tumors, yet the effects of this treatment on the normal urinary bladder microcirculation have not been determined. This study involved the use of in vivo television microscopy to observe the effects of light activation (530 to 560 nm., 175 mW/cm.) on the urinary bladder microcirculation of female Sprague-Dawley rats. Animals pretreated with dihematoporphyrin ether 30 minutes prior to light activation had high serum and low tissue concentrations of the photosensitizer and activation resulted in a statistically significant reduction of red blood cell column diameter in both arterioles and venules. The reduction was primarily due to mural thrombus formation with the occlusion remaining 60 minutes after activation. Animals pretreated 48 hours before activation had low serum and high tissue concentrations of dihematoporphyrin ether and activation had no microcirculatory effects apart from occasional platelet aggregation. These results suggest that the photodynamic effect on normal (non-neoplastic) tissue treated more than 48 hours after administration of dihematoporphyrin ether is probably due to a direct effect of light and the sensitizer on the smooth muscle of the bladder rather than an effect on the microcirculation.
Reed
M W
MW
Dept. of Physiology and Biophysics, School of Medicine, University of Louisville, KY 40292.
Schuschke
D A
DA
Ackermann
D M
DM
Harty
J I
JI
Wieman
T J
TJ
Miller
F N
FN
D000818:Animals; D001160:Arterioles / Q000187:drug effects; D017323:Dihematoporphyrin Ether; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects; D005260:Female; D006415:Hematoporphyrins / Q000494:pharmacology; D008833:Microcirculation / Q000187:drug effects; D010778:Photochemotherapy; D051381:Rats; D011919:Rats, Inbred Strains; D013927:Thrombosis / Q000139:chemically induced; D001743:Urinary Bladder / Q000098:blood supply*; D014661:Vasoconstriction; D014699:Venules / Q000187:drug effects
D006415:Hematoporphyrins; D017323:Dihematoporphyrin Ether