Isolation and characterization of (gamma, delta) CD4+ T cell clones derived from human fetal liver cells.
Lymphocytes isolated from human fetal liver and expanded in vitro in IL-2-containing media reveal the existence of CD4+ gamma, delta T cells. These cells display differential features of double-negative and CD8+ gamma, delta T cells as well as of CD4+ alpha, beta T cells. Thus, they failed to lyse targets in lectin-mediated killing assays and to perform classical helper functions. These results add new information necessary for a better understanding of the physiological role of the gamma, delta T cells.
Aparicio
P
P
Centro de Biología Molecular, C.S.I.C., Universidad Autónoma, Madrid, Spain.
Alonso
J M
JM
Toribio
M L
ML
Marcos
M A
MA
Pezzi
L
L
Martínez-A
C
C
D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis*; D017252:CD3 Complex; D016827:CD8 Antigens; D002469:Cell Separation; D002999:Clone Cells; D003602:Cytotoxicity, Immunologic; D005333:Fetus / Q000276:immunology*; D008099:Liver / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000032:analysis; D013601:T-Lymphocytes / Q000502:physiology*
D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D016827:CD8 Antigens; D011948:Receptors, Antigen, T-Cell