In vivo formation of beta-oxidized metabolites of leukotriene E4 in the rat.
Intraperitoneal administration of [3H]-leukotriene E4 in the rat resulted in the appearance of radiolabel in urine and feces. Separation of polar urinary metabolites and chromatographic comparison of synthetic metabolites indicated the in vivo formation of omega-oxidized metabolites of LTE4 with sequential beta-oxidation. Furthermore, the metabolite identified as 16-carboxy-17,18,19,20-tetranor-14,15-dihydro-N-acetyl-LTE4 substantiates the biochemical pathway of beta-oxidation in vivo involving the 2,4-dienoyl CoA reductase as an integral step. These results substantiate beta-oxidation of sulfidopeptide leukotrienes in vivo and these metabolites account for some of the major urinary metabolites of this class of lipid mediator.
Perrin
P
P
Dept. of Pharmacology, Univ. of Colorado Health Sciences Center, Denver 80262.
Zirrolli
J
J
Stene
D O
DO
Lellouche
J P
JP
Beaucourt
J P
JP
Murphy
R C
RC
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D044943:Fatty Acid Desaturases / Q000378:metabolism; D005243:Feces / Q000032:analysis; D017999:Leukotriene E4; D008297:Male; D010084:Oxidation-Reduction; D044925:Oxidoreductases Acting on CH-CH Group Donors; D051381:Rats; D011919:Rats, Inbred Strains; D013189:SRS-A / Q000031:analogs & derivatives* / Q000378:metabolism / Q000652:urine; D014316:Tritium
D013189:SRS-A; D014316:Tritium; D017999:Leukotriene E4; D044943:Fatty Acid Desaturases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C100153:2,4-dienoyl-CoA reductase