title: Proto-oncogene analyses in brain tumors.
abstract: The present study determined which oncogenes (N-myc, c-myc, v-sis, or v-fos) were amplified and which messenger ribonucleic acids (mRNA's) accumulated in 10 primary human brain tumors of neuroectodermal origin. The tumors included four glioblastomas multiforme, one mixed glioma (astrocytoma grade I and ependymoma), one astrocytoma grade II, one cystic cerebellar astrocytoma, one ependymoma, one ganglioglioma, and one medulloblastoma. The relative amounts of polyadenylated (poly(A)+) RNA's homologous to these genes and their copy number were determined using the RNA and deoxyribonucleic acid blot hybridization techniques. The N-myc and v-sis probes hybridized strongly to the poly(A)+ RNA from the same recurrent glioblastoma with gene amplifications (N-myc 80 copies; v-sis three to four copies). The c-myc probe hybridized strongly to the recurrent medulloblastoma without gene amplification. The amplification or abundant accumulation of mRNA's homologous to their oncogenes may be involved in tumorigenesis or the aggressiveness of these malignant brain tumors of neuroectodermal origin and may be good molecular indicators of an extremely malignant state in these tumors.
authors.affiliation: Department of Surgery, Division of Neurosurgery, University of Texas Health Science Center, San Antonio.
authors.forename: M
authors.initials: M
authors.lastname: Fujimoto
authors.forename: P J
authors.initials: PJ
authors.lastname: Sheridan
authors.forename: Z D
authors.initials: ZD
authors.lastname: Sharp
authors.forename: F J
authors.initials: FJ
authors.lastname: Weaker
authors.forename: S
authors.initials: S
authors.lastname: Kagan-Hallet
authors.forename: J L
authors.initials: JL
authors.lastname: Story
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001254:Astrocytoma / Q000235:genetics*; D001932:Brain Neoplasms / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D004247:DNA; D004806:Ependymoma / Q000235:genetics; D005260:Female; D005784:Gene Amplification; D005909:Glioblastoma / Q000235:genetics*; D005910:Glioma / Q000235:genetics; D006801:Humans; D008297:Male; D008527:Medulloblastoma / Q000235:genetics; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D000090063:Proto-Oncogene Mas; D011519:Proto-Oncogenes; D012333:RNA, Messenger; D013120:Spinal Cord Neoplasms / Q000235:genetics
chemical_list: C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger; D004247:DNA