Antibodies specific to the retinoic acid human nuclear receptors alpha and beta.
Two cDNAs encoding two human receptors for retinoic acid (RA), RAR-alpha and RAR-beta, have been characterized recently. Synthetic peptides corresponding to the cDNA-deduced amino acid sequences unique to RAR-alpha and RAR-beta were used to generate anti-RAR-alpha antiserum (SP171) and anti-RAR-beta antisera (SP172 and SP248). The specificity of these antisera was confirmed both by immunocytochemical detection of these receptors in COS-1 cells transfected with RAR-alpha and RAR-beta expression vectors and by immunoblot analyses performed with whole extracts of these cells. We also demonstrate that these antisera recognize RAR-alpha and RAR-beta endogenously expressed in the RA-responsive human promyelocytic leukemia cell line HL-60.
Gaub
M P
MP
Unité 184 de Biologie Moléculaire et de Génie Génétique de Institut National de la Santé et de la Recherche Médicale, Institut de Chimie Biologique, Faculté de Médecine, Strasbourg, France.
Lutz
Y
Y
Ruberte
E
E
Petkovich
M
M
Brand
N
N
Chambon
P
P
D000595:Amino Acid Sequence; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity; D015153:Blotting, Western; D002352:Carrier Proteins / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D004247:DNA / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D015473:Leukemia, Promyelocytic, Acute / Q000378:metabolism; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology; D018168:Receptors, Retinoic Acid; D014162:Transfection; D014407:Tumor Cells, Cultured
D000906:Antibodies; D002352:Carrier Proteins; D010446:Peptide Fragments; D018168:Receptors, Retinoic Acid; D004247:DNA