Effect of hemodialysis on T lymphocyte subsets, con-A-activated suppressor cell activity, and interleukin-2 receptor expression on lymphocytes in chronic uremic patients.
Effect of hemodialysis (HD) on some indices of immune response was studied in nine chronic uremics. Total lymphocyte, OKT4+, and OKT8+ cell numbers significantly decreased during the first 20 min of HD, and they were decreased till the third hour of the procedure, whereas the OKT4+/OKT8+ cell number ratio did not change significantly. Before HD, Con-A--activated suppressor cells exerted a stimulatory action on autologous responder cells measured in two-step culture. During HD, Con-A-activated suppressor cell activity transiently appeared, with its peak at 60 min after the start of HD. It was accompanied by a transient rise in lymphocyte count with spontaneous interleukin-2 (IL-2) receptor expression, whereas the number of cells expressing IL-2 receptor following phytohemaglutinin (PHA) stimulation was progressively decreased during HD. A significant correlation was found between the increment of Con-A-activated suppressor cell activity and the increment of spontaneous IL-2 receptor expression on lymphocytes during one single blood flow through the dialyzer. The results supply further evidence that HD may impose additional disturbances on immune regulation in chronic uremics.
Tchórzewski
H
H
Department of Pathophysiology, Institute of Basic Medical Sciences, WAM, Lódź, Poland.
Luciak
M
M
Trznadel
K
K
Majewska
E
E
Pokoca
L
L
D000328:Adult; D002908:Chronic Disease; D003208:Concanavalin A; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D008213:Lymphocyte Activation; D008297:Male; D015375:Receptors, Interleukin-2 / Q000032:analysis*; D006435:Renal Dialysis / Q000009:adverse effects*; D013601:T-Lymphocytes / Q000145:classification*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D014511:Uremia / Q000276:immunology*
D015375:Receptors, Interleukin-2; D003208:Concanavalin A