The testes of moles (Talpa europaea) retain a considerable microsomal capacity for androgen synthesis during seasonal regression.
Seasonal changes in testicular histology and steroidogenesis were investigated in the mole (Talpa europaea). Androgen synthesis was examined by incubating [4-14C]pregnenolone (P) and [4-14C]dehydroepiandrosterone (DHA) with testicular minces in a static incubation system. The metabolites formed were characterized by thin-layer chromatography. Morphological changes were studied by routine histological methods. During sexual quiescence spermatogenesis was arrested. The regressive seminiferous tubules consisted predominantly of Sertoli cells and spermatogonia. On the other hand, histological quantification suggested that season has no significant effects on the number or the nuclear dimensions of Leydig cells in this species. The capacity of the regressive testes (per unit weight) to metabolize P and DHA to testosterone (T) was somewhat greater in regressive (48.5%, 49.4%) than in active (33.2%, 41.6%) testes. The results also suggest that the greater in vitro T production encountered during reproductive quiescence is due possibly to an increase in the activity of 17 beta-hydroxysteroid dehydrogenase (per unit weight). Our data on Leydig cell numbers indicate, however, that the capacity of the individual Leydig cells to produce T is decreased during sexual regression. T. europaea appears to be quite exceptional among seasonally breeding small mammals exhibiting pronounced annual changes in spermatogenesis in that the testes retain a considerable enzymatic capacity to produce testosterone from pregnanes during sexual quiescence. The results suggest that pituitary as well as paracrine regulation of the annual testicular cycle in this species differs from that generally encountered in seasonal breeders.
Tähkä
K M
KM
Division of Physiology, University of Helsinki, Finland.
Teräväinen
T
T
Pankakoski
E
E
Jalkanen
M
M
D000728:Androgens / Q000378:metabolism*; D000818:Animals; D003687:Dehydroepiandrosterone / Q000378:metabolism; D007312:Eulipotyphla / Q000378:metabolism*; D008297:Male; D008861:Microsomes / Q000378:metabolism*; D008971:Moles / Q000378:metabolism*; D009929:Organ Size; D011284:Pregnenolone / Q000378:metabolism; D012621:Seasons; D013737:Testis / Q000033:anatomy & histology / Q000166:cytology / Q000378:metabolism*
D000728:Androgens; D003687:Dehydroepiandrosterone; D011284:Pregnenolone