Stimulation of human neutrophilic granulocytes by two monocyte-derived cytokines.
Human monocytes upon stimulation with bacterial lipopolysaccharide release two cytokines which modulate the functions of neutrophilic granulocytes (PMN), a monocyte-derived chemotaxin (MOC) and tumor necrosis factor (TNF). Both cytokines stimulated the adherence of PMN on protein-coated nylon-fibers. Whereas MOC is one of the four most potent chemoattractants known, TNF was a most powerful inhibitor of PMN chemotactic migration towards several chemotactic factors including MOC. Neither cytokine stimulated the release of superoxide anion (O2-) or hydrogen peroxide (H2O2) from PMN in suspension. However, TNF, but not MOC, caused the release of considerable amounts of H2O2 and O2- from PMN attached to nylon fibers. The two cytokines have similar effects on the adherence, opposing effects on chemotactic migration and different effects on the oxidative burst of PMN.
Kownatzki
E
E
Abteilung für Experimentelle Dermatologie, Universitäts-Hautklinik, Freiburg, FRG.
Neumann
M
M
Uhrich
S
S
D001685:Biological Factors / Q000494:pharmacology*; D016207:Cytokines; D006098:Granulocytes / Q000502:physiology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D009000:Monocytes / Q000502:physiology*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology*
D001685:Biological Factors; D016207:Cytokines; D014409:Tumor Necrosis Factor-alpha; D013481:Superoxides; D009240:N-Formylmethionine Leucyl-Phenylalanine; D006861:Hydrogen Peroxide