Labelling of monoclonal antibodies 10B, 8C, and M2A with indium-111.
Monoclonal antibodies 10B, 8C and M2A were conjugated with cyclic DTPA anhydride to a substitution level of 1-2 mol DTPA/mol antibody. The DTPA-conjugated antibodies were purified by size-exclusion mini-column chromatography and labelled with [111In]In acetate to a specific activity of 37-74 kBq/micrograms (1-2 microCi/micrograms). The radiochemical purity of the antibody preparation was 76-81%. The immunoreactive fraction of the radiolabelled antibodies ranged from 0.17 to 0.31, significantly lower than that of their radioiodinated analogs.
Reilly
R
R
Toronto General Hospital, Division of Nuclear Medicine, Ontario, Canada.
Sheldon
K
K
Marks
A
A
Houle
S
S
D000911:Antibodies, Monoclonal; D002460:Cell Line; D005260:Female; D006801:Humans; D007205:Indium Radioisotopes; D007553:Isotope Labeling / Q000379:methods*; D010051:Ovarian Neoplasms / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007205:Indium Radioisotopes