Deglycosylation and proteolysis of photolabeled D2 dopamine receptors of the porcine anterior pituitary.
Dopamine D2 receptor binding subunits of the porcine anterior pituitary were visualized by autoradiography following photoaffinity labeling with [125I]N-azidophenethylspiperone and sodium dodecyl-sulfate polyacrylamide gel electrophoresis. The ligand binding subunit comprising the pituitary D2 dopamine receptor migrated as two distinct bands of apparent Mr approximately equal to 150,000 and 118,000, substantially higher than neuronal D2 receptor subunits from porcine or canine brain. The glycoprotein nature of pituitary D2 receptor binding subunits was investigated by the use of exo- and endo-glycosidase treatments and peptide mapping experiments. Photoaffinity labeled polypeptides of the anterior pituitary were susceptible to both neuraminidase and alpha-mannosidase digestion as indexed by their increased electrophoretic mobility on sodium dodecyl-sulfate polyacrylamide gels, and suggests the presence of both complex type and terminal mannose carbohydrate residues. Moreover, the additive effects of sequential treatment with these enzymes suggests that both types of carbohydrate chains are present on each receptor peptide. N-linked deglycosylation of pituitary D2 photolabeled receptors with glycopeptidase-F produced a further increase in the mobility of the labeled protein to apparent Mr approximately equal to 44,000, similar to that of deglycosylated D2 binding subunits of porcine and canine brain. Peptide mapping experiments following limited proteolysis with Staphylococcus aureus V8 proteinase and papain demonstrated that deglycosylated D2 dopamine receptors (Mr = 44,000), in different tissues and species, were homologous. Taken together, these data suggest that despite the differences in the overall molecular weight and tissue specific glycosylation pattern of pituitary D2 dopamine receptors, the primary structure of mammalian D2 receptors appears to be conserved.
Jarvie
K R
KR
Department of Pharmacology, University of Toronto, Ontario, Canada.
Niznik
H B
HB
D000818:Animals; D003342:Corpus Striatum / Q000648:ultrastructure; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D006026:Glycoside Hydrolases / Q000378:metabolism; D007457:Iodine Radioisotopes; D008361:Mannosidases / Q000378:metabolism; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D008562:Membrane Glycoproteins / Q000378:metabolism; D008970:Molecular Weight; D009439:Neuraminidase / Q000378:metabolism; D010449:Peptide Mapping; D010903:Pituitary Gland, Anterior / Q000648:ultrastructure*; D011954:Receptors, Dopamine / Q000032:analysis / Q000378:metabolism*; D017448:Receptors, Dopamine D2; D013134:Spiperone / Q000031:analogs & derivatives; D013552:Swine
D007457:Iodine Radioisotopes; D008562:Membrane Glycoproteins; D011954:Receptors, Dopamine; D017448:Receptors, Dopamine D2; C053754:azido-N-(4-aminophenethyl)spiperone; D013134:Spiperone; D006026:Glycoside Hydrolases; D008361:Mannosidases; D009439:Neuraminidase; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase