An enzyme-linked immunoassay for the measurement of rat alpha 1-acid glycoprotein synthesized by cultured hepatocytes.
We have developed a sandwich ELISA to quantify rat alpha 1-acid glycoprotein (AGP). The assay correlated well with RID and the minimum detectable concentration was 1 microgram/l. The assay permits high sensitivity determinations of the rate of synthesis of AGP in vitro. The maximum mean rates observed were 1500 and 1800 ng/24 h/10(6) cells for hepatocytes cultured alone and co-cultured hepatocytes respectively and 39 ng/h/10(6) cells for isolated hepatocytes.
Biou
D
D
Laboratoire de Biochimie (URA 017), U.E.R. Sciences Pharmaceutiques, Chatenay-Malabry, France.
Daveau
M
M
Rigal
O
O
Hiron
M
M
Porquet
D
D
Lebreton
J P
JP
D000818:Animals; D002478:Cells, Cultured; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005779:Immunodiffusion; D008099:Liver / Q000378:metabolism; D009961:Orosomucoid / Q000032:analysis*; D051381:Rats
D009961:Orosomucoid