Superinduction of the murine B cell Fc epsilon RII by T helper cell clones. Role of IL-4.
Th cell clones are known to induce an IL-4 dependent polyclonal IgE synthesis. Because IL-4 can induce the expression of the low affinity FcR for IgE (Fc epsilon RII) the ability of Th cell clones to induce Fc epsilon RII on purified splenic B cells was analyzed. It was found that a TH2 clone could cause a 50- to 100-fold superinduction of Fc epsilon RII after 2 days in culture; after 3 days, the Fc epsilon RII levels had almost returned to base line. The superinduction was inhibited by an anti-IL-4 antibody, 11B11, indicating its dependence on IL-4. A TH1 clone could cause a modest (four fold) induction of Fc epsilon RII, and this induction was not influenced by 11B11. A similar Fc epsilon RII induction was seen when using the supernatant from activated TH1 cells. The component(s) causing this relatively low level Fc epsilon RII induction is not known; a variety of known lymphokines were tested, and only IL-4 demonstrated any capacity for Fc epsilon RII induction on LPS-activated B cells. Addition of rIL-4 at concentrations of 400 U/ml or greater to the TH1 culture was sufficient to cause a Fc epsilon RII superinduction similar to that seen with the TH2 clone, while 40 U/ml was not. In order to determine a potential role for the Fc epsilon RII or its soluble fragment on the IgE synthesis mediated by TH2, a monoclonal anti-Fc epsilon RII, B3B4, was added to the culture. The addition of B3B4 did not have an influence on IgE levels in this system.
Keegan
A D
AD
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Snapper
C M
CM
Van Dusen
R
R
Paul
W E
WE
Conrad
D H
DH
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology; D000941:Antigens / Q000276:immunology; D000944:Antigens, Differentiation, B-Lymphocyte / Q000096:biosynthesis* / Q000276:immunology / Q000502:physiology; D001402:B-Lymphocytes / Q000378:metabolism*; D002999:Clone Cells / Q000378:metabolism; D007073:Immunoglobulin E / Q000096:biosynthesis / Q000378:metabolism*; D015847:Interleukin-4; D007378:Interleukins / Q000494:pharmacology / Q000502:physiology*; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008809:Mice, Inbred C3H; D008811:Mice, Inbred DBA; D011961:Receptors, Fc / Q000096:biosynthesis* / Q000276:immunology / Q000502:physiology; D017455:Receptors, IgE; D006377:T-Lymphocytes, Helper-Inducer / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D000941:Antigens; D000944:Antigens, Differentiation, B-Lymphocyte; D007378:Interleukins; D011961:Receptors, Fc; D017455:Receptors, IgE; D015847:Interleukin-4; D007073:Immunoglobulin E