title: Glycemia and immunohistochemical changes in the endocrine pancreas of the turtle Chrysemys dorbigni treated with streptozotocin.
abstract: The diabetogenic action of streptozotocin (SZ) was investigated in the turtle Chrysemys dorbigni after a 1- or 14-day fast. SZ (130 or 250 mg/kg) was injected intravenously, and blood glucose and plasma insulin were measured. Pancreatic endocrine cells were stained immunohistochemically by the immunoperoxidase avidin-biotin-peroxidase complex method. Only 14% of the SZ-treated turtles showed hyperglycemia. Prolonged fasting did not increase the percentage of hyperglycemic animals. In control turtles, insulin (beta)-, glucagon (alpha)- and somatostatin (delta)-immunoreactive cells were detected in increasing order of frequency. The qualitative changes seen in cells from the hyperglycemic SZ-treated turtles were more evident in beta and delta cells.
authors.affiliation: Departamento de Patologia, Faculdade de Medicina, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brasil.
authors.forename: W W
authors.initials: WW
authors.lastname: Muniz
authors.forename: M
authors.initials: M
authors.lastname: Marques
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis*; D005215:Fasting; D005260:Female; D007150:Immunohistochemistry; D007328:Insulin / Q000097:blood*; D007515:Islets of Langerhans / Q000187:drug effects* / Q000473:pathology; D008297:Male; D013311:Streptozocin / Q000494:pharmacology*; D014426:Turtles / Q000097:blood*
chemical_list: D001786:Blood Glucose; D007328:Insulin; D013311:Streptozocin