title: Inhibition of superoxide and ferritin-dependent lipid peroxidation by ceruloplasmin.
abstract: Ceruloplasmin (CP) was found to inhibit xanthine oxidase and ferritin-dependent peroxidation of phospholipid liposomes, as evidenced by decreased malondialdehyde formation. Ceruloplasmin was also shown to inhibit superoxide-mediated mobilization of iron from ferritin, in a concentration-dependent manner, as measured spectrophotometrically using the iron(II) chelator bathophenanthroline sulfonate. Ceruloplasmin failed to function as a peroxyl radical-scavenging antioxidant as evidenced by its inability to inhibit free radical-initiated peroxidation of linoleic acid, suggesting that CP inhibited lipid peroxidation by affecting the availability of ferritin-derived iron. In addition, CP scavenged xanthine oxidase-derived superoxide as measured spectrophotometrically via its effect on cytochrome c reduction. However, the extent of the superoxide scavenging of CP did not quantitatively account for its effects on iron release, suggesting that CP inhibits superoxide-dependent mobilization of ferritin iron independently of its ability to scavenge superoxide. The effects of CP and apoferritin on iron-catalyzed lipid peroxidation in systems containing exogenously added ferrous iron was also investigated. In the absence of apoferritin, CP exhibited a concentration-dependent prooxidant effect. However, CP-dependent, iron-catalyzed lipid peroxidation was inhibited by the addition of apoferritin. Apoferritin did not function as a peroxyl radical-scavenging antioxidant but was shown to incorporate iron in the presence of CP. These data suggest that CP inhibits superoxide and ferritin-dependent lipid peroxidation largely via its ability to reincorporate reductively mobilized iron back into ferritin.
authors.affiliation: Biotechnology Center, Utah State University, Logan 84322-4430.
authors.forename: V M
authors.initials: VM
authors.lastname: Samokyszyn
authors.forename: D M
authors.initials: DM
authors.lastname: Miller
authors.forename: D W
authors.initials: DW
authors.lastname: Reif
authors.forename: S D
authors.initials: SD
authors.lastname: Aust
mesh_terms: D000818:Animals; D002570:Ceruloplasmin / Q000494:pharmacology*; D003574:Cytochrome c Group / Q000037:antagonists & inhibitors; D005293:Ferritins / Q000378:metabolism*; D007700:Kinetics; D015227:Lipid Peroxidation / Q000187:drug effects*; D008081:Liposomes; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013482:Superoxide Dismutase / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism*; D014969:Xanthine Oxidase / Q000378:metabolism
chemical_list: D003574:Cytochrome c Group; D008081:Liposomes; D013481:Superoxides; D005293:Ferritins; D013482:Superoxide Dismutase; D002570:Ceruloplasmin; D014969:Xanthine Oxidase