title: Mechanisms underlying the organizer formation in Bufo arenarum embryos.
abstract: In the early gastrula of Bufo arenarum the prospective mesoderm was previously identified as a marginal belt of grey cells. To analyze their differentiation capacity explants of these cells were cultured within ectodermal vesicles, in isolation and in combination with vegetal components. When cultured in isolation, dorsal and ventral fragments from the deep marginal zone behaved differently. Whilst ventral explants produced blood cells, dorsal explants failed to differentiate, remaining as masses of yolk-laden cells. On the other hand, both cultures were drastically modified when associated with superficial cells from the blastoporal zone, which caused the following effects: a) Promotion of differentiation in dorsal marginal explants, able now to produce notochordal and somitic structures, in addition to mesenchymatic cells. b) Promotion of dorsalization in ventral marginal explants, which changed their expected destiny developing axial components, similar to those furnished by "activated" dorso marginal explants. On the contrary, combined cultures of animal and vegetal pieces were unable to generate mesodermal structures. These studies suggest that the axial mesoderm, identified as the "organizer", develops from a marginal substrate of genuine mesodermal cells through a dorsalizing inductive stimulus originated in superficial periblastoporal cells.
authors.forename: M E
authors.initials: ME
authors.lastname: Manes
authors.forename: O L
authors.initials: OL
authors.lastname: Nieto
mesh_terms: D000818:Animals; D002022:Bufo arenarum / Q000196:embryology*; D002454:Cell Differentiation; D004475:Ectoderm / Q000502:physiology; D004627:Embryonic Induction; D005775:Gastrula / Q000502:physiology / Q000648:ultrastructure; D008648:Mesoderm / Q000502:physiology* / Q000648:ultrastructure; D009924:Organ Culture Techniques