title: Voltage-dependent calcium current in adherent mouse 3T3 fibroblasts.
abstract: Whole-cell recording was performed on adherent mouse Swiss 3T3 fibroblasts. Depolarizations from a holding potential of -100 mV gave rise to a transient inward current. The voltage dependence, kinetic properties, and ionic selectivity of this current are identical to those described in the same cells kept in suspension after detachment from the culture dish [A. Pandiella, A. Malgaroli, J. Meldolesi, and L.M. Vicentini (1987) Exp. Cell. Res. 170, 175-185; W.H. Moolenaar, L.G.J. Tertoolen, and S.W. de Laat (1984) J. Biol. Chem. 259, 8066-8069].
authors.affiliation: Department of General Physiology and Biochemistry, University of Milan, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Peres
authors.forename: E
authors.initials: E
authors.lastname: Sturani
authors.forename: R
authors.initials: R
authors.lastname: Zippel
mesh_terms: D000200:Action Potentials; D000818:Animals; D002104:Cadmium / Q000502:physiology; D015220:Calcium Channels / Q000502:physiology*; D002448:Cell Adhesion; D002460:Cell Line; D004594:Electrophysiology; D005347:Fibroblasts / Q000502:physiology*; D008274:Magnesium / Q000502:physiology; D051379:Mice
chemical_list: D015220:Calcium Channels; D002104:Cadmium; D008274:Magnesium