title: Ultracytochemical localization of Ca2+-ATPase activity in reactive astrocytes.
abstract: Ca2+-ATPase activity on the astrocyte plasma membrane was investigated ultracytochemically, using the lead salt technique. Normal astrocytes showed a weak cytochemical reaction for Ca2+-ATPase activity, deposits of the reaction product being small. At 7 and 15 days after cold lesioning, reactive astrocytes apparently in the process of repair of the edematous lesion were observed; these demonstrated an intense cytochemical reaction for Ca2+-ATPase activity in their plasma membranes facing the extracellular fluid, with reaction product accumulation. At 2 months, the lesion had progressed to glial scars containing sporadic microcysts. The reactive astrocytes surrounding the microcysts demonstrated a moderate cytochemical reaction for Ca2+-ATPase activity in their free plasma membranes, whereas those arranged in a cell-to-cell pattern showed little reaction product in their plasma membranes. In conclusion, a more intense cytochemical reaction was always observed in the free plasma membrane of reactive astrocytes.
authors.affiliation: Department of Pathology, Niigata University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kawai
authors.forename: H
authors.initials: H
authors.lastname: Takahashi
authors.forename: K
authors.initials: K
authors.lastname: Wakabayashi
authors.forename: F
authors.initials: F
authors.lastname: Ikuta
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000201:enzymology* / Q000648:ultrastructure; D001927:Brain Diseases / Q000201:enzymology / Q000473:pathology*; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology; D002540:Cerebral Cortex / Q000201:enzymology; D006651:Histocytochemistry; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000252:Calcium-Transporting ATPases