Studies on the biogenesis of the mammalian ATP synthase complex: isolation and characterization of a full-length cDNA encoding the rat F1-beta-subunit.
Screening a rat cDNA library with a fragment of the gene encoding the corresponding bovine protein, we have isolated cDNA clones encoding the complete precursor to the rat F1-beta-subunit. Comparison with other mammalian beta-subunit sequences reveals a 5' untranslated sequence of 27 bp, a targeting sequence of 48 amino acid residues, a 480 amino acid long mature polypeptide and a 3' untranslated region of approximately 150 bp. In vitro translation of F1-beta mRNA reveals the synthesis of multiple fragments of the protein. Preliminary characterization of intron-containing cDNA isolates indicates that the gene encoding the rat F1-beta subunit contains at least two introns.
Boulet
D
D
Department of Chemistry, Université du Québec à Montréal, Canada.
Poirier
J
J
Côté
C
C
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008099:Liver / Q000201:enzymology*; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D006180:Proton-Translocating ATPases / Q000235:genetics*; D051381:Rats; D015183:Restriction Mapping; D014158:Transcription, Genetic
D046911:Macromolecular Substances; D004247:DNA; D006180:Proton-Translocating ATPases