Preservation of factor VIII activity in plasma by different freezing techniques, as determined with a chromogenic microtray assay.
The preservation of factor VIII activity in frozen plasma was studied using three different freezing techniques. Two were conventional methods, i.e. ethanol bath at -40 degrees C, and deep-frozen at -80 degrees C, and the third was a novel air-based -40 degrees C freezer with metal compartments and snugly fitting flat 750 ml plasma containers. No statistically significant difference in preservation of factor VIII was observed, irrespective of freezing technique, as determined with a chromogenic substrate assay.
Vaara
I
I
Department of Clinical Chemistry, Central Hospital, Växjö, Sweden.
Jonsson
M
M
D002863:Chromogenic Compounds / Q000378:metabolism; D004355:Drug Stability; D000431:Ethanol; D005169:Factor VIII / Q000378:metabolism*; D005615:Freezing; D006801:Humans; D010949:Plasma / Q000378:metabolism*; D013696:Temperature
D002863:Chromogenic Compounds; D000431:Ethanol; D005169:Factor VIII