In vitro phenotypic and functional characterization of human pigment epithelial cell lines.
We have characterized human retinal pigment epithelium (HRPE) for the expression of cell surface antigens. Primary HRPE cultures, established cell lines, and freshly brushed pigment epithelial cells all express HLA-ABC but not HLA-DR antigens. However, both primary cultures and established cell lines can be induced by gamma interferon stimulation to express HLA-DR in a dose dependent manner. Only freshly brushed HRPE cells express Fc, and no cells demonstrated the presence of C3b. Our results show that HRPE cells change in culture, as reflected by the loss of Fc receptors, but retain the ability to synthesize HLA-ABC spontaneously and HLA-DR upon stimulation.
Dutt
K
K
Morehouse School of Medicine, Department of Pathology, Atlanta, GA 30310.
Waldrep
J C
JC
Kaplan
H J
HJ
Del Monte
M
M
Semple
E
E
Verly
G
G
D000293:Adolescent; D000368:Aged; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis*; D002460:Cell Line; D002478:Cells, Cultured; D002648:Child; D006680:HLA Antigens / Q000032:analysis; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D010641:Phenotype; D010857:Pigment Epithelium of Eye / Q000276:immunology*; D011951:Receptors, Complement / Q000032:analysis; D017463:Receptors, Complement 3b; D011961:Receptors, Fc / Q000032:analysis
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D006680:HLA Antigens; D006684:HLA-DR Antigens; D011951:Receptors, Complement; D017463:Receptors, Complement 3b; D011961:Receptors, Fc