Characterization of a purF operon mutation which affects colicin V production.
A mini-Tn10-kan insertion mutation identified a gene in the chromosome of Escherichia coli required for colicin V production from plasmid pColV-K30. With the complete restriction map of E. coli, the mutation was rapidly mapped to 50.0 min, within the purF operon. Sequence analysis showed that the insertion occurred in a gene with no previously known function which is located directly upstream of purF. We designated this gene cvpA for colicin V production. The mutant requires adenine for growth, probably because of a polar effect on purF expression. However, an adenine auxotroph showed no defect in colicin V production, suggesting that the cvpA mutation is responsible for the effect on colicin V production. Two possible models of cvpA1 allele function are discussed.
Fath
M J
MJ
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115.
Mahanty
H K
HK
Kolter
R
R
D000483:Alleles; D003086:Bacteriocin Plasmids; D002876:Chromosomes, Bacterial / Q000502:physiology; D003001:Cloning, Molecular; D003087:Colicins / Q000096:biosynthesis*; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D009876:Operon; D010957:Plasmids; D011687:Purines / Q000502:physiology; D015183:Restriction Mapping
D003087:Colicins; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D011687:Purines