Binding of the neurotrophic peptide Org 2766 to rat spinal cord sections is affected by a sciatic nerve crush.
The binding of the neurotrophic peptide, [3H]Org 2766 (55 nM), to rat spinal cord sections was studied, employing quantitative autoradiography. The binding was unevenly distributed over spinal cord structures and was displaceable by non-labelled Org 2766 to a limited extent (35%). Binding could not be displaced by the opiate antagonist, naloxone, indicating that [3H]Org 2766 binding sites are distinct from opiate receptors. However, the exact nature of the binding sites remains to be elucidated. A marked left-right difference in [3H]Org 2766 binding in the dorsal horns of the spinal cord at level L2 was observed, 6 days after unilateral crush lesioning of the sciatic nerve. No such effect was found at level T10. After 28 days, when sensorimotor functioning had completely recovered, the [3H]Org 2766 binding pattern was comparable to that in sham-operated rats again. It is suggested that Org 2766 binds to axonal sprouts or glia in the dorsal horn of the spinal cord.
Dekker
A J
AJ
CNS Pharmacology Department, Organon International, Oss, The Netherlands.
Tonnaer
J A
JA
D000324:Adrenocorticotropic Hormone / Q000031:analogs & derivatives* / Q000378:metabolism; D000818:Animals; D000927:Anticonvulsants / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D010446:Peptide Fragments / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012584:Sciatic Nerve / Q000502:physiology*; D013116:Spinal Cord / Q000378:metabolism*; D014316:Tritium
D000927:Anticonvulsants; D010446:Peptide Fragments; D014316:Tritium; C013765:Org 2766; D000324:Adrenocorticotropic Hormone