Negative-ion mass spectrometry of substituted adenine bases and adenosine nucleosides.
The negative-ion chemical ionization (ammonia, 5 Pa source pressure) mass spectra of a series of substituted adenine bases, adenosine nucleosides, and the trimethylsilyl derivatives of the nucleosides are described. Selected ions from these spectra were subject to collisionally activated dissociation with mass-analysed ion kinetic energy (CAD/MIKE) analysis of the products and the spectra assessed for information content. In addition to observing strong peaks due to quasimolecular ions and heterocyclic-base ions, it proved possible to differentiate between 2'-, 3'- and 5'-deoxy and between 2'- and 3'-O-methyl isomers. The negative-ion chemical ionization spectra of four methyladenines are essentially identical, but could be clearly distinguished from each other by CAD/MIKE analysis.
Hocart
C H
CH
Universität Bern, Institute für Organische Chemie, Switzerland.
Schlunegger
U P
UP
D000225:Adenine / Q000031:analogs & derivatives / Q000737:chemistry*; D000241:Adenosine / Q000031:analogs & derivatives / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods
D000225:Adenine; D000241:Adenosine