title: Expression of the dnaN and dnaQ genes of Escherichia coli is inducible by mitomycin C.
abstract: The dnaN and dnaQ genes encode the beta subunit and the epsilon subunit of the DNA polymerase III holoenzyme. Using translational fusions to lacZ we found that DNA damage caused by mitomycin C induces expression of the dnaA and dnaQ genes. This induction was not observed in lexA and recA mutants which block the induction of the SOS response, suggesting a relationship between the mechanism(s) of genetic control of DNA polymerase III holoenzyme and the SOS regulatory network. Nevertheless, there is evidence that the mitomycin C induction of dnaN and dnaQ is not a simple lexA-regulated process, because nalidixic acid (an excellent SOS inducer) does not increase dnaN and dnaQ gene expression, and the time course of induction is abnormally slow.
authors.affiliation: Wissenschaftsbereich Genetik, Martin-Luther-Universität, Halle/Saale, German Democratic Republic.
authors.forename: M
authors.initials: M
authors.lastname: Kaasch
authors.forename: J
authors.initials: J
authors.lastname: Kaasch
authors.forename: A
authors.initials: A
authors.lastname: Quiñones
mesh_terms: D004249:DNA Damage; D004258:DNA Polymerase III / Q000096:biosynthesis / Q000235:genetics*; D004260:DNA Repair; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D004790:Enzyme Induction / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D005472:Fluorouracil / Q000494:pharmacology; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology*; D009154:Mutation; D009268:Nalidixic Acid / Q000494:pharmacology; D013014:SOS Response, Genetics
chemical_list: D008937:Mitomycins; D009268:Nalidixic Acid; D016685:Mitomycin; D004258:DNA Polymerase III; D004259:DNA-Directed DNA Polymerase; D005472:Fluorouracil