Cepabactin from Pseudomonas cepacia, a new type of siderophore.
In iron-deficient conditions of growth Pseudomonas cepacia ATCC 25416 excreted both pyochelin and a low-molecular-mass compound which strongly chelated iron(III), and facilitated iron translocation as demonstrated by growth and uptake experiments. The name cepabactin is proposed for this new siderophore. Comparisons of UV-visible spectra and chromatographic behaviour, together with 1H-NMR spectra, led to the conclusion that cepabactin is 1-hydroxy-5-methoxy-6-methyl-2(1H)-pyridinone, a compound which can be considered as a cyclic hydroxamate, but also as a heterocyclic analogue of catechol. This pyridinone has already been described by other workers as an antibiotic produced by Pseudomonas alcaligenes, and by a soil isolate closely related to Pseudomonas cepacia. Thus, cepabactin appears to act as a siderophore for more than one species of non-fluorescent pseudomonad.
Meyer
J M
JM
Institut de Biologie Moléculaire et Cellulaire du Centre National de la Recherche Scientifique, Strasbourg, France.
Hohnadel
D
D
Hallé
F
F
D007501:Iron / Q000378:metabolism; D007502:Iron Chelating Agents / Q000302:isolation & purification* / Q000494:pharmacology; D010636:Phenols / Q000302:isolation & purification; D011549:Pseudomonas / Q000032:analysis* / Q000187:drug effects / Q000254:growth & development; D017262:Siderophores; D013329:Structure-Activity Relationship; D013386:Succinates / Q000378:metabolism; D019802:Succinic Acid; D013844:Thiazoles
D007502:Iron Chelating Agents; D010636:Phenols; D017262:Siderophores; D013386:Succinates; D013844:Thiazoles; C025316:pyochelin; D019802:Succinic Acid; D007501:Iron