Formation of IgE-binding factors by lymphocytes of HIV-infected patients.
Peripheral blood mononuclear cells of human immunodeficiency virus infected patients were cultured in the presence or absence of 10 micrograms/ml human IgE, and culture filtrates were fractionated on IgE-Sepharose. IgE-binding factors were assessed in the acid eluate fraction from IgE-Sepharose by both rosette inhibition assay and radioimmunoassay. Among 22 cases studied, mononuclear cells from 9 patients formed IgE-binding factors in the absence of IgE, and those of 13 cases formed the factors upon incubation with IgE. The major cell source of IgE-binding factors was T cells. In 2 cases of human immunodeficiency virus infection, Fc epsilon receptors were detected on both Leu-2+ and Leu-3+ T cells.
Carini
C
C
Johns Hopkins University School of Medicine, Baltimore, Md.
Margolick
J
J
Yodoi
J
J
Ishizaka
K
K
D000386:AIDS-Related Complex / Q000276:immunology; D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000378:metabolism; D006679:HIV Seropositivity / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism*; D008222:Lymphokines / Q000096:biosynthesis*; D029584:Prostatic Secretory Proteins; D011863:Radioimmunoassay; D011961:Receptors, Fc / Q000378:metabolism; D017455:Receptors, IgE; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*
D000944:Antigens, Differentiation, B-Lymphocyte; D008222:Lymphokines; D029584:Prostatic Secretory Proteins; D011961:Receptors, Fc; D017455:Receptors, IgE; C043076:beta-microseminoprotein; C013059:immunoglobulin-binding factors; D007073:Immunoglobulin E