[Modification of DNA with dihydrazide of succinic acid for preparation of hybridization probes].
A two-step chemical method of introduction of nonradioactive labels in DNA was proposed. At first step DNA is modified by succinic dihydrazide at pH 5.0 and 95 degrees C, or at pH 4.5 and 37 degrees C in presence of sodium bisulfite. Then FITC or biotin are joined to the hydrazide groups. DNA modified in this way were shown to be effective hybridisation probes.
Turchinskiĭ
M F
MF
Aĭnbinder
E I
EI
Knorre
V D
VD
Shcherbo
S N
SN
D010582:Bacteriophage lambda / Q000235:genetics; D001710:Biotin; D055598:Chemical Phenomena; D002621:Chemistry; D015342:DNA Probes; D004279:DNA, Viral / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D006834:Hydrazines / Q000378:metabolism / Q000494:pharmacology*; D007202:Indicators and Reagents; D009693:Nucleic Acid Hybridization; D013386:Succinates / Q000378:metabolism / Q000494:pharmacology*; D013447:Sulfites; D013604:T-Phages / Q000235:genetics
D015342:DNA Probes; D004279:DNA, Viral; D006834:Hydrazines; D007202:Indicators and Reagents; D013386:Succinates; D013447:Sulfites; C063357:succinic dihydrazide; D001710:Biotin; C009279:sodium bisulfite
Modifikatsiia DNK digidrazidom iantarnoĭ kisloty dlia polucheniia gibridizatsionnykh zondov.