Adenine nucleotide contents and ATPases activities in porcine deciduous dental pulp during the root formation, in fully formed root and during root resorption phases.
Nucleotide content and activity of certain enzymes were compared in pigs of various ages in order to study the energetic metabolism of deciduous dental pulps in the three phases of the cycle of tooth ontogeny, namely, root formation, fully formed root and root resorption phases. The frozen pulps were removed with the help of a screw vise and analysed for ATP, ADP and AMP contents and Ca2+ and Mg2+-ATPases activities. The highest ATP content in the first deciduous molar pulp was found when the tooth was still in an intrabony position. The calculated energy charge, although low for all groups, at this stage of development, indicated an activation of the consuming processes. In the root resorption phase, lowest ATP content and higher Ca2+ and Mg2+-ATPases activities were observed.
Paiva-Novaes
M S
MS
Department of Biochemistry, University of Sao Paulo, Brasil.
Ferreira
F D
FD
Nicolau
J
J
D000227:Adenine Nucleotides / Q000032:analysis*; D000244:Adenosine Diphosphate / Q000032:analysis; D000249:Adenosine Monophosphate / Q000032:analysis; D000255:Adenosine Triphosphate / Q000032:analysis; D000375:Aging / Q000502:physiology*; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000032:analysis*; D000252:Calcium-Transporting ATPases / Q000032:analysis*; D003782:Dental Pulp / Q000032:analysis* / Q000201:enzymology; D005260:Female; D008297:Male; D008963:Molar / Q000032:analysis* / Q000201:enzymology; D012391:Root Resorption / Q000201:enzymology / Q000503:physiopathology*; D013552:Swine; D014092:Tooth Root / Q000032:analysis* / Q000196:embryology / Q000201:enzymology; D014094:Tooth, Deciduous / Q000032:analysis* / Q000201:enzymology
D000227:Adenine Nucleotides; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases