Furazolidone-induced interstrand cross-links in Vibrio cholerae DNA. Study of conformational change by circular dichroism.
Vibrio cholerae DNA bearing furazolidone-induced interstand cross-links show a change in the characteristic circular dichroism spectra of the DNA itself in dilute buffer. The change in c.d. spectra was characterized by a shift of the positive band around 272 nm to lower wavelength and a loss of ellipticity of the negative band around 242 nm, and is similar to that exhibited by mitomycin linked Vibrio cholerae DNA under identical conditions and is suggestive of a conformational change of DNA bearing such cross-links. Both furazolidone-induced and mitomycin-induced cross-linking of Vibrio cholerae DNA inhibited the salt-induced conformation change, i.e. increase in winding angle of DNA, the percentage inhibition being greater for mitomycin-linked DNA.
Chatterjee
S N
SN
Biophysics Division, Saha Institute of Nuclear Physics, Calcutta, India.
Basak
J
J
Maiti
M
M
D002021:Buffers; D002942:Circular Dichroism; D003432:Cross-Linking Reagents; D004269:DNA, Bacterial / Q000737:chemistry*; D005664:Furazolidone / Q000494:pharmacology*; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D009690:Nucleic Acid Conformation; D009994:Osmolar Concentration; D012965:Sodium Chloride; D014734:Vibrio cholerae / Q000235:genetics*
D002021:Buffers; D003432:Cross-Linking Reagents; D004269:DNA, Bacterial; D008937:Mitomycins; D012965:Sodium Chloride; D016685:Mitomycin; D005664:Furazolidone