GTP gamma S stimulation of endosome fusion suggests a role for a GTP-binding protein in the priming of vesicles before fusion.
Guanosine 5'-(3-O-thio)triphosphate (GTP gamma S), a non-hydrolyzable analogue of GTP, inhibits in vitro fusion among early endocytic vesicles in the presence of high concentrations of cytosol. In this report we show that fusion is remarkably stimulated by GTP gamma S under conditions where cytosolic components are the limiting factors for the process. The amount of cytosolic factors required for maximal fusion activity is several-fold decreased by the presence of GTP gamma S. Moreover, preincubation of vesicles in the presence of cytosol and GTP gamma S allows fusion to proceed even in the absence of cytosol. Our results indicate that a GTP-binding protein facilitates the binding of cytosolic factor(s) required for endosome fusion to the endosomal membrane and stabilizes a dilution-resistant intermediate of the fusion process.
Mayorga
L S
LS
Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri 63110.
Diaz
R
R
Colombo
M I
MI
Stahl
P D
PD
D000818:Animals; D002999:Clone Cells; D003600:Cytosol / Q000378:metabolism; D004705:Endocytosis / Q000187:drug effects* / Q000502:physiology; D019204:GTP-Binding Proteins / Q000378:metabolism; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology*; D007700:Kinetics; D008561:Membrane Fusion / Q000187:drug effects* / Q000502:physiology; D008854:Microscopy, Electron
D016244:Guanosine 5'-O-(3-Thiotriphosphate); D019204:GTP-Binding Proteins