Modulation of growth factor induced fiber outgrowth in rat pheochromocytoma (PC12) cells by a fibronectin receptor antibody.
Rat pheochromocytoma PC12 cells respond to the binding of nerve growth factor (NGF) and basic fibroblast growth factor (bFGF) by extending neurites in a manner resembling sympathetic neurons. This response requires cell attachment to an appropriate substratum (Fujii et al., J. Neurosci., 2:1157, 1982); attachment factors which function in this capacity include the adhesive proteins fibronectin and laminin. Incubating PC12 cells with a polyclonal antiserum directed against a putative 140-kDa fibroblast cell surface fibronectin receptor (anti-gp140) perturbed spreading but not attachment of the cells to fibronectin and laminin substrates. However, in the presence of anti-gp 140 or its Fab fragments, NGF-stimulated neurite outgrowth was dramatically reduced. The antibody also caused a retraction of previously extended neurites. SDS-PAGE analysis of immunoprecipitates of PC12 cells surface labeled with 125I identified a prominent 120-140-kDa band, suggesting that the site of anti-gp140 action in PC12 cells is also through a fibronectin receptor.
Schwarz
M A
MA
Department of Biological Chemistry, College of Medicine, University of California, Irvine 92717.
Brown
P J
PJ
Eveleth
D D
DD
Bradshaw
R A
RA
D000310:Adrenal Gland Neoplasms / Q000473:pathology*; D000818:Animals; D000906:Antibodies; D002448:Cell Adhesion; D002460:Cell Line; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D005353:Fibronectins / Q000378:metabolism; D007797:Laminin / Q000378:metabolism; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D010673:Pheochromocytoma / Q000473:pathology*; D051381:Rats; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic / Q000276:immunology*
D000906:Antibodies; D005353:Fibronectins; D007797:Laminin; D009414:Nerve Growth Factors; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic; D005346:Fibroblast Growth Factors