Abnormal glucocorticoid receptor gene and mRNA in primary cortisol resistance.
Abnormal steroid hormone receptors have been implicated as causing several forms of primary steroid hormone resistance in humans, but as yet no abnormality has been described at the gene level. We describe the analysis of the mRNA and genomic DNA from the Epstein-Barr (EB) virus transformed cells of two siblings with Primary Cortisol Resistance. The cells of the propositus and his brother show a decreased level of glucocorticoid receptor (GR) mRNA, and the genomic DNA of both individuals shows an altered restriction enzyme pattern with the restriction enzyme Bgl II, one of eleven restriction enzymes tested. The genomic differences could be detected with a probe specific for the putative steroid binding domain of the human GR gene.
Linder
M J
MJ
Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77550-2779.
Thompson
E B
EB
D002472:Cell Transformation, Viral; D004247:DNA / Q000032:analysis; D015342:DNA Probes; D004351:Drug Resistance; D004854:Herpesvirus 4, Human; D006801:Humans; D006854:Hydrocortisone / Q000378:metabolism*; D008297:Male; D009710:Nucleotide Mapping; D012333:RNA, Messenger / Q000032:analysis*; D011965:Receptors, Glucocorticoid / Q000235:genetics*
D015342:DNA Probes; D012333:RNA, Messenger; D011965:Receptors, Glucocorticoid; D004247:DNA; D006854:Hydrocortisone