Inhibition of in vivo genotoxicity by coffee.
The possible role of coffee in modulating the in vivo genotoxicity of the well established genotoxic chemicals, mitomycin C, cyclophosphamide, procarbazine and adriamycin, was evaluated. Coffee was administered orally to mice that received the genotoxic chemicals ip. Genotoxicity was assessed in the bone-marrow micronucleus test. Doses of coffee in the range 225 to 1125 mg (dry weight)/kg body weight caused significant reductions in the in vivo genotoxicity of mitomycin C, cyclophosphamide and procarbazine but not adriamycin. The inhibitory effect was significant when the coffee was given about 2 hr before the genotoxin; there was a lesser effect when coffee was given together with the genotoxin but there was no inhibition when coffee was given 2-4 hr after the genotoxin. An experiment with mitomycin C demonstrated that the reduction in genotoxicity was dependent on the coffee dose. The inhibition of genotoxicity by coffee was observed in bone-marrow cells sampled 24, 48 or 68 hr after injecting cyclophosphamide. Freshly brewed coffee extract, standard instant coffee, decaffeinated instant coffee and freeze-dried home-brew coffee all exerted inhibitory effects.
Abraham
S K
SK
School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
D000704:Analysis of Variance; D000818:Animals; D003069:Coffee; D003520:Cyclophosphamide / Q000037:antagonists & inhibitors; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000037:antagonists & inhibitors; D005260:Female; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D016685:Mitomycin; D008937:Mitomycins / Q000037:antagonists & inhibitors; D009153:Mutagens / Q000037:antagonists & inhibitors*; D011344:Procarbazine / Q000037:antagonists & inhibitors
D003069:Coffee; D008937:Mitomycins; D009153:Mutagens; D011344:Procarbazine; D016685:Mitomycin; D004317:Doxorubicin; D003520:Cyclophosphamide