[Radioreceptorassay for human growth hormone in serum].
Radioreceptorassay (RRA) for serum human growth hormone (hGH) using late pregnancy rabbit liver receptors is reported. The sensitivity was 90 +/- 11 pg per tube with high specificity and without cross-reactivity with other human pituitary hormones except prolactin (2.4% cross-reactivity). Accuracy of the assay was good and dilution curve of acromegalic serum parallel with standard curve. The coefficients of variation for intra- and inter-assay were 11.3-13.3 and 7.7-18.8%, respectively. The mean concentration of RRA hGH in 64 normal adults was 1.2 +/- 0.3 ng/ml. The level of RRA hGH was less than 1 ng/ml in 57 of 64 normal controls, while that of RRA hGH in 23 acromegalic sera was 98.7 +/- 33.7 ng/ml, the difference being highly significant (P less than 0.01). Thus, RRA hGH is a good index reflecting functional state of hGH.
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JY
D000172:Acromegaly / Q000097:blood; D000328:Adult; D000818:Animals; D005260:Female; D013006:Growth Hormone / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011817:Rabbits; D011869:Radioligand Assay / Q000379:methods*; D011986:Receptors, Somatotropin / Q000097:blood
D011986:Receptors, Somatotropin; D013006:Growth Hormone