title: Phosphorylation of avian retrovirus matrix protein by Ca2+/phospholipid-dependent protein kinase.
abstract: The matrix protein from avian myeloblastosis virus and the Rous sarcoma virus, Prague C strain, is a phosphoprotein. A comparison of the amino acid sequences shows these phosphoproteins are very similar. The sites of phosphorylation of the matrix protein purified from virions are identified as serine residues 68 and 106. Treatment with purified rabbit skeletal-muscle protein phosphatase 1 or 2A, selectively releases phosphate from serine 68, while alkali treatment releases phosphate from both sites. When analyzed as a substrate for six different protein kinases, only the Ca2+/phospholipid-dependent protein kinase modifies the matrix protein. The serine residues phosphorylated in vivo are identical to those phosphorylated in vitro by this protein kinase. The role of these phosphorylation events in viral production is discussed.
authors.affiliation: Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, OH 44106.
authors.forename: J
authors.initials: J
authors.lastname: Leis
authors.forename: N
authors.initials: N
authors.lastname: Phillips
authors.forename: X
authors.initials: X
authors.lastname: Fu
authors.forename: P T
authors.initials: PT
authors.lastname: Tuazon
authors.forename: J A
authors.initials: JA
authors.lastname: Traugh
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001354:Avian Leukosis Virus / Q000378:metabolism*; D009189:Avian Myeloblastosis Virus / Q000378:metabolism*; D001358:Avian Sarcoma Viruses / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D003488:Cyanogen Bromide; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis; D010449:Peptide Mapping; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism*; D011817:Rabbits; D012191:Retroviridae Proteins / Q000378:metabolism*
chemical_list: D010446:Peptide Fragments; D012191:Retroviridae Proteins; D011493:Protein Kinase C; D003488:Cyanogen Bromide