Light-dark differences in [3H]-paroxetine binding to rabbit platelet membranes.
[3H]-Paroxetine binding to rabbit blood platelet membranes from samples obtained under light and dark conditions was examined. Animals were kept on a 14 h light (L) - 10 h dark (D) schedule and blood samples were collected at L + 7 and D + 5 h. Significant differences were found for Bmax values of [3H]-paroxetine binding, with low Bmax values during the light period and high Bmax values during the dark period. The Kd values were not significantly different. These results confirm previous observations on light-dark differences of [3H]-imipramine binding in rabbit blood platelets suggesting the existence of a circadian rhythm for the 5-HT transporter complex.
Rocca
P
P
Department of Biology, Synthélabo Recherche, Paris, France.
Galzin
A M
AM
Langer
S Z
SZ
D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002940:Circadian Rhythm; D003624:Darkness; D007099:Imipramine / Q000378:metabolism; D066298:In Vitro Techniques; D008027:Light; D008297:Male; D017374:Paroxetine; D010880:Piperidines / Q000378:metabolism*; D011817:Rabbits; D012702:Serotonin Antagonists / Q000378:metabolism*
D010880:Piperidines; D012702:Serotonin Antagonists; D017374:Paroxetine; D007099:Imipramine