Monocyte-lymphocyte discrimination in a new microtitre-based adhesion assay.
A new, rapid and reproducible method is described for the quantification of in vitro monocyte adherence in microtitre 96-well ELISA plates. The assay is based on the measurement of the myeloperoxidase activity in monocytes that have adhered to either plastic or bovine/porcine aortic endothelial cells. The assay shows a linear response to monocyte concentrations over the range 5 x 10(4)-5 x 10(5) cells/well. The method provides good discrimination between monocyte and and lymphocytes and thus allows a study of mononuclear cell populations to be made. The assay works equally well with polymorphonuclear cells. The coefficients of variation are: intra-assay, 7%; interassay, 13%.
Bath
P M
PM
Department of Medicine, King's College Hospital, London, U.K.
Booth
R F
RF
Hassall
D G
DG
D002448:Cell Adhesion; D004730:Endothelium, Vascular / Q000166:cytology; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000494:pharmacology; D008077:Lipoproteins, LDL / Q000494:pharmacology; D008214:Lymphocytes / Q000166:cytology* / Q000201:enzymology; D009000:Monocytes / Q000166:cytology* / Q000201:enzymology; D009195:Peroxidase / Q000032:analysis*
D007375:Interleukin-1; D008077:Lipoproteins, LDL; D009195:Peroxidase