title: Erythrocyte insulin receptor characteristics and erythrocyte membrane lipid composition in healthy men.
abstract: The cell membrane plays an important role in the mechanism of insulin action. To test whether erythrocyte insulin receptor characteristics are related to the erythrocyte membrane lipid composition, 11 healthy volunteers were studied. The relationship between insulin binding to erythrocytes, the number of receptors per cell and the affinity of receptors to insulin on the one hand and total phospholipid fatty acid (FA) composition and cholesterol/phospholipid molar ratio in the erythrocyte membrane on the other hand were evaluated. 1. We found a significant negative correlation between specific insulin binding and the proportion of n-6 essential FA in erythrocyte membrane phospholipids, especially linoleic acid (r = -0.82, p less than 0.01) and arachidonic acid (r = -0.73, p less than 0.05). On the other hand, a significant positive correlation between insulin binding and the proportion of nonessential FA (r = +0.65, p less than 0.05) was seen. Number of receptors per cell and the affinity of receptors were not significantly related to phospholipid FA composition. 2. There was no significant correlation between insulin receptor characteristics and the cholesterol/phospholipid molar ratio in the erythrocyte membrane. The data presented support the hypothesis that the FA pattern of membrane total phospholipids may modify the properties of insulin receptors.
authors.affiliation: Institute for Clinical and Experimental Medicine, Faculty of General Medicine, Charles University, Prague.
authors.forename: T
authors.initials: T
authors.lastname: Pelikánová
authors.forename: M
authors.initials: M
authors.lastname: Kohout
authors.forename: J
authors.initials: J
authors.lastname: Hilgertová
authors.forename: J
authors.initials: J
authors.lastname: Base
authors.forename: I
authors.initials: I
authors.lastname: Farská
authors.forename: J
authors.initials: J
authors.lastname: Válek
mesh_terms: D000328:Adult; D002784:Cholesterol / Q000032:analysis; D004910:Erythrocyte Membrane / Q000032:analysis* / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D008297:Male; D008563:Membrane Lipids / Q000032:analysis*; D008875:Middle Aged; D010743:Phospholipids / Q000032:analysis; D011972:Receptor, Insulin / Q000032:analysis / Q000378:metabolism*
chemical_list: D005227:Fatty Acids; D007328:Insulin; D008563:Membrane Lipids; D010743:Phospholipids; D002784:Cholesterol; D011972:Receptor, Insulin