A human embryo fibroblast-derived peptide suppresses the zymosan-induced biochemical activation in human polymorphonuclear leukocytes.
Previously, a low-molecular-weight peptide was found in the serum-free culture media conditioned by a human embryo fibroblast cell strain (YH-1), which showed inhibitory effects on the proliferation and functions of B and T lymphocytes (Okai et al. (1987) Zool. Sci. 4, 99-105). In this paper, the inhibitory effect of this peptide on the functional activation in human polymorphonuclear leukocytes (PMNs) is documented. This peptide suppressed the opsonized zymosan-induced generation of chemiluminescence and superoxide anion and concomitant RNA synthesis. However, this peptide did not show significant cytotoxic effects on human PMNs as judged by a cytoplasmic enzyme-releasing assay. These results indicate that a fibroblast-derived peptide reported previously has a suppressive activity on the functional activation of human PMNs. The immunological significance of this finding is discussed.
Okai
Y
Y
Toxicological Research Laboratories, Kyowa Hakko Kogyo Co., Yamaguchi, Japan.
D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000032:analysis*; D006801:Humans; D008163:Luminescent Measurements; D008213:Lymphocyte Activation / Q000187:drug effects*; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D013481:Superoxides / Q000096:biosynthesis; D014158:Transcription, Genetic / Q000187:drug effects; D015054:Zymosan / Q000037:antagonists & inhibitors*
D010455:Peptides; D013481:Superoxides; D015054:Zymosan