title: Identification of a transforming gene of human papillomavirus type 16.
abstract: Previously, we observed sequential two-step alteration, growth stimulation, and progression to a more malignant state in NIH 3T3 cells transfected by human papillomavirus type 16 (HPV-16) DNA. In this study, we prepared a cDNA library from RNA extracted from cells transfected with the HPV-16 DNA and isolated cDNA clones which had growth-stimulating activity. Analysis of these cDNA clones indicated that the E7 open reading frame alone is responsible for inducing both steps of this cell transformation.
authors.affiliation: Institute for Virus Research, Kyoto University, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Tanaka
authors.forename: T
authors.initials: T
authors.lastname: Noda
authors.forename: H
authors.initials: H
authors.lastname: Yajima
authors.forename: M
authors.initials: M
authors.lastname: Hatanaka
authors.forename: Y
authors.initials: Y
authors.lastname: Ito
mesh_terms: D000818:Animals; D002478:Cells, Cultured / Q000166:cytology / Q000382:microbiology; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005814:Genes, Viral; D006133:Growth Substances / Q000235:genetics*; D009857:Oncogenes; D027383:Papillomaviridae / Q000235:genetics*; D051381:Rats
chemical_list: D006133:Growth Substances; D004247:DNA