A method for intratracheal instillation of endotoxin into the lungs of mice.
A detailed method is presented for the intratracheal instillation of fluids into the lungs of mice. Using 125I-albumen it was determined that 25-50 microliters of volume gave almost equal distribution to all lobes of the mouse lung. Extremely low levels of endotoxin given intratracheally (12.5-25 ng) resulted in a dramatic increase in the neutrophil content of the lung. The increase started in less than 4 h, reaching levels of 7 x 10(5) by 18 h and was maintained at elevated levels for at least 48 h. This neutrophil count represented more than 90% of the cell population of the lung.
Starcher
B
B
Department of Biochemistry, University of Texas Health Center, Tyler 75710.
Williams
I
I
D000818:Animals; D004195:Disease Models, Animal; D004731:Endotoxins / Q000008:administration & dosage*; D007442:Intubation, Intratracheal / Q000662:veterinary*; D007958:Leukocyte Count; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL / Q000502:physiology*; D009504:Neutrophils; D011656:Pulmonary Emphysema / Q000209:etiology* / Q000473:pathology; D012711:Serum Albumin, Radio-Iodinated; D013047:Specific Pathogen-Free Organisms
D004731:Endotoxins; D012711:Serum Albumin, Radio-Iodinated