Study of the N-glycoprotein biosynthesis through dolichol intermediates in the mitochondrial membranes.
1. In the mitochondria, the biosynthesis of N-glycoprotein products, through the dolichol intermediates pathway, appears in the outer and in the inner membranes. 2. The biosynthesis of dolichol-pyrophosphoryl-N-acetyl-glucosamine, dolichol-pyrophosphoryl-di-N-acetylchitobiose, dolichol-phosphoryl-glucose and dolichol-phosphoryl-mannose is effective in both membranes. 3. The lipid-linked oligosaccharides biosynthesized in both membranes contain high mannose-type oligosaccharides ranging in size from Man9-GlcNac2 to Man4-GlcNac2. 4. The assembly of the dolichol-pyrophosphoryl-oligosaccharides on the trimannosidic core begins by the elongation of the alpha-1,3 mannose branch in the outer membrane and of the alpha-1,6 mannose branch in the inner membrane.
Levrat
C
C
Department of Biochemistry, University of Lyon, Lyon-Sud Medical School, INSERM-CNRS U.189, Oullins, France.
Ardail
D
D
Morelis
R
R
Louisot
P
P
D017485:1-Deoxynojirimycin; D000118:Acetylglucosaminidase; D000818:Animals; D002458:Cell Fractionation; D002853:Chromatography, Liquid / Q000379:methods; D004286:Dolichols / Q000378:metabolism*; D005944:Glucosamine / Q000031:analogs & derivatives / Q000494:pharmacology; D006023:Glycoproteins / Q000096:biosynthesis*; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000378:metabolism; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D051379:Mice; D008928:Mitochondria / Q000378:metabolism*
D004286:Dolichols; D006023:Glycoproteins; D017485:1-Deoxynojirimycin; D000118:Acetylglucosaminidase; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D005944:Glucosamine