Defective virus is associated with induction of murine retrovirus-induced immunodeficiency syndrome.
C57BL/6 mice infected with a mixture of murine leukemia viruses (MuLV) develop a syndrome characterized by lymphoproliferation and profound immunodeficiency. Analyses of this viral mixture (LP-BM5 MuLV) showed that it includes replication-competent ecotropic and mink cell focus-inducing MuLV and defective viruses with genome sizes of 3.8-6.5 kilobases. The ecotropic and mink cell focus-inducing MuLV biologically cloned from the mixture did not induce disease, whereas viral preparations containing the ecotropic MuLV and 4.8-kilobase defective virus were active. Cells producing the 4.8-kilobase defective virus expressed an unusual gag-encoded polyprotein of Mr 60,000.
Chattopadhyay
S K
SK
Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892.
Morse
H C
HC
Makino
M
M
Ruscetti
S K
SK
Hartley
J W
JW
D000163:Acquired Immunodeficiency Syndrome / Q000662:veterinary*; D000818:Animals; D004279:DNA, Viral / Q000235:genetics; D003673:Defective Viruses / Q000472:pathogenicity*; D015683:Gene Products, gag; D007153:Immunologic Deficiency Syndromes / Q000382:microbiology*; D009052:Leukemia Virus, Murine / Q000235:genetics / Q000472:pathogenicity*; D051379:Mice; D008810:Mice, Inbred C57BL; D015183:Restriction Mapping; D012191:Retroviridae Proteins / Q000235:genetics
D004279:DNA, Viral; D015683:Gene Products, gag; D012191:Retroviridae Proteins