title: Collaboration between human blood dendritic cells and monocytes in antigen presentation.
abstract: We compared human blood dendritic cells and monocytes for their capacity to produce secreted and membrane interleukin 1 (IL-1), stimulate mixed leukocyte reaction (MLR) and augment microbial antigen-induced T lymphocyte proliferation. Our enriched dendritic cell and monocyte fractions contained greater than 80% and greater than 93% dendritic cells and monocytes, respectively. Monocytes produced about ten times higher amounts of membrane and secreted IL-1 than dendritic cells, which in turn were more potent in presenting HLA-DR antigens in MLR. Both accessory cell types presented purified protein derivative of tuberculin (PPD) equally well, whereas monocytes were better with fixed Bacillus Calmette Guérin (BCG) bacteria. Processing of BCG was chloroquine-sensitive. Coculture experiments suggested that there was collaboration or synergy between dendritic cells and monocytes in antigen processing and presentation.
authors.affiliation: Department of Biomedical Sciences, University of Tampere, Finland.
authors.forename: L
authors.initials: L
authors.lastname: Räsänen
authors.forename: M
authors.initials: M
authors.lastname: Lehto
authors.forename: H
authors.initials: H
authors.lastname: Hyöty
authors.forename: P
authors.initials: P
authors.lastname: Leinikki
mesh_terms: D000938:Antigen-Presenting Cells / Q000502:physiology*; D000942:Antigens, Bacterial / Q000276:immunology; D003713:Dendritic Cells / Q000502:physiology*; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D009000:Monocytes / Q000502:physiology*
chemical_list: D000942:Antigens, Bacterial; D007375:Interleukin-1; D007376:Interleukin-2