Establishment and characterization of a subline predisposed to pulmonary metastasis from a human gestational choriocarcinoma cell line in nude mice.
A subline predisposed to pulmonary metastasis was successfully derived by repeating in vivo selection of GCH-1, a human gestational choriocarcinoma cell line, in nude mice. While the parent line GCH-1, transplanted subcutaneously to nude mice, induced pulmonary metastasis in a few of the host animals, the newly established subline GCH-1(m) successfully metastasized to the lungs in 100% of them. Compared with GCH-1, GCH-1(m) exhibited a higher degree of cell atypia, a lower capacity for cell growth and markedly higher productivity for human chorionic gonadotropin (hCG). Another characteristic of this subline was its enhanced growth after the addition of a pulmonary extract obtained from nude mice. The relationship of these findings to the mechanisms of metastasis of this cancer was discussed.
Kanazawa
K
K
Department of Obstetrics and Gynecology, Niigata University School of Medicine, Japan.
Adachi
S
S
Yoshiya
N
N
Honma
S
S
Takahashi
H
H
Takeuchi
S
S
D000818:Animals; D002460:Cell Line; D002822:Choriocarcinoma / Q000473:pathology / Q000556:secondary*; D005260:Female; D006801:Humans; D008168:Lung / Q000473:pathology; D008175:Lung Neoplasms / Q000556:secondary*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011247:Pregnancy; D014594:Uterine Neoplasms / Q000473:pathology*