Determination of encainide and its metabolites by high-performance liquid chromatography.
Encainide (ENC) and its metabolites O-demethylencainide (ODE), 3-methoxy-O-demethylencainide (MODE), N-demethylencainide (NDE) and bis-N,O-demethylencainide (NODE) have been measured by two HPLC procedures. The method of Mayol using a mu Porasil column with ethanol-water-methanesulphonic acid as mobile phase was not able to separately measure NODE and NDE in plasma. A new method is described using a mu Bondapak Phenyl column with acetonitrile-phosphate buffer (0.05 M, pH 7.5) that yields satisfactory separation of ENC and its metabolites. NODE was not identified as a metabolite in 23 patients analysed.
Selinger
K
K
Royal Victoria Hospital and McGill University, Division of Clinical Biochemistry, Quebec, Canada.
Crawhall
J C
JC
D000813:Anilides / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D000889:Anti-Arrhythmia Agents / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D003640:Dealkylation; D016700:Encainide; D006801:Humans; D013056:Spectrophotometry, Ultraviolet
D000813:Anilides; D000889:Anti-Arrhythmia Agents; D016700:Encainide