Elevated levels of the guanine nucleotide binding protein, Go, are associated with differentiation of neuroblastoma x glioma hybrid cells.
Each of a range of pharmacological agents which function to increase intracellular levels of cAMP caused a morphological 'differentiation' of neuroblastoma x glioma hybrid, NG108-15, cells grown in tissue culture. Associated with this differentiation, increased incorporation of [32P]ADP-ribose catalysed by pertussis toxin was noted into a band of some 39-40 kDa in membranes derived from these cells. Immunoblotting using two antipeptide antisera which identify different regions of Go alpha demonstrated marked increases in the levels of this polypeptide in membranes of the differentiated cells. However, levels of the beta-subunit did not increase appreciably with differentiation.
Mullaney
I
I
Department of Biochemistry, University of Glasgow, Scotland.
Milligan
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G
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000527:Alprostadil / Q000494:pharmacology; D015153:Blotting, Western; D003994:Bucladesine / Q000494:pharmacology; D002454:Cell Differentiation; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D019204:GTP-Binding Proteins / Q000378:metabolism*; D005910:Glioma / Q000378:metabolism*; D006822:Hybrid Cells / Q000378:metabolism*; D008970:Molecular Weight; D009243:NAD / Q000494:pharmacology; D009447:Neuroblastoma / Q000378:metabolism*; D037342:Pertussis Toxin; D014407:Tumor Cells, Cultured; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D010566:Virulence Factors, Bordetella; D009243:NAD; D005576:Colforsin; D000246:Adenosine Diphosphate Ribose; D003994:Bucladesine; D000242:Cyclic AMP; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins; D000527:Alprostadil