title: Beta-endorphin-like immunoreactivity levels in the hypothalamus, the periaqueductal grey and the pituitary of the DBA mouse: determination by ELISA and relationship to nociception.
abstract: The present paper describes the development and application of an enzyme-linked immunosorbent assay (ELISA) for the assessment of beta-endorphin-like immunoreactivity (beta-ELIR) level in the hypothalamus, the periaqueductal grey (PAG) and the pituitary of DBA/2 mice that were subjected to mild social stress (aggressive confrontation). After confrontation these subjects showed elevated tail-flick latencies (TFL) when compared to controls, a finding that indicates stress-induced analgesia (SIA). A positive correlation was found between individual TFLs and beta-ELIR levels in the PAG but not in the hypothalamus and the pituitary. These results suggest that individual baseline PAG beta-ELIR levels may be taken as a predictor of high degrees of stress-induced analgesia.
authors.affiliation: Institute of Forensic Medicine, University of Zurich, Switzerland.
authors.forename: P
authors.initials: P
authors.lastname: Külling
authors.forename: B
authors.initials: B
authors.lastname: Siegfried
authors.forename: H R
authors.initials: HR
authors.lastname: Frischknecht
authors.forename: F S
authors.initials: FS
authors.lastname: Messiha
authors.forename: A
authors.initials: A
authors.lastname: Pasi
mesh_terms: D000378:Agonistic Behavior / Q000502:physiology; D000818:Animals; D001143:Arousal / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay; D007031:Hypothalamus / Q000502:physiology*; D007124:Immunoenzyme Techniques; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D008813:Mice, Inbred ICR; D009619:Nociceptors / Q000502:physiology*; D010487:Periaqueductal Gray / Q000502:physiology*; D010902:Pituitary Gland / Q000502:physiology*; D011930:Reaction Time / Q000502:physiology; D013045:Species Specificity; D001615:beta-Endorphin / Q000502:physiology*
chemical_list: D001615:beta-Endorphin