Further study of pollen typhae's effects on the production of tPA and PGI, by cultured endothelial cells.
We had reported that Pollen Typhae, a Chinese traditional herbal medicine could reduce atherosclerotic plaque formation in experimental animals. Fraction IV isolated from Pollen Typhae had the ability to raise tPA and PGI2 productions by cultured porcine aortic endothelial cells (EC). In this paper we reported that F.IV was divided chemically into four subfractions named SubF.1-4 among them SubF.2-2 was further separated from SubF.2, and SubF.4-1 from SubF.4. The six subfractions were tested for their effects on the productions of tPA (125I-fibrin plate method) and PGI2(RIA) by porcine endothelial cells. The results showed that SubF.3 could enhance tPA activity and SubF.2-2 could increase PGI2 level. With Chrono-Log 500 Lumi-Aggregometer, SubF.1,2-2 and 3 were found to be able to inhibit platelet aggregation and release reaction. These data indicated that the mechanisms of Pollen Typhae in its antiatherosclerotic effects were multiple which resulted from various biologic effects exerted by various components of this drug. Chemical analyses were performed and their structures were identified, demonstrating that SubF.2-2 was a flavonoid, while SubF.3 was a group of flavonoids, implying there are some antiatherogenic components in flavonoids.
Zhao
J
J
Shanghai Institute of Hematology, People's Republic of China.
Zhang
C Y
CY
Xu
D M
DM
Huang
G Q
GQ
Xu
Y L
YL
Wang
Z Y
ZY
Fang
S D
SD
Chen
Y
Y
Gu
Y L
YL
D000328:Adult; D000818:Animals; D001161:Arteriosclerosis / Q000517:prevention & control; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism; D011464:Epoprostenol / Q000096:biosynthesis*; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D010974:Platelet Aggregation / Q000187:drug effects; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis*
D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D011464:Epoprostenol; D010959:Tissue Plasminogen Activator