title: Membrane responses of B-16 melanoma cells to single exposure to ultraviolet light.
abstract: Electron spin resonance spectroscopy using the spin probe (5-, 12- and 16-deoxylstearic acid) was employed to analyze the changes in membrane fluidity in B-16 melanoma cells following UV-B exposure. The UV exposure resulted in the immediate accumulation of lipid peroxide, being accompanied by a change in membrane fluidity. The 12-DSA is the most sensitive to the changes in membrane organization caused by UV light. Na+,K+-ATPase activity was regulated by a change in membrane fluidity. Following UV exposure, the release of the prelabeled arachidonic acid from the cells was observed immediately. Ca2+-dependent calmodulin-dependent phospholipase A2-like activity was involved in the UV-stimulated arachidonic acid release from phospholipid.
authors.affiliation: Department of Medical Biochemistry, Kurume University School of Medicine, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Ogura
authors.forename: M
authors.initials: M
authors.lastname: Sugiyama
authors.forename: T
authors.initials: T
authors.lastname: Sakanashi
authors.forename: T
authors.initials: T
authors.lastname: Suematsu
authors.forename: T
authors.initials: T
authors.lastname: Hidaka
authors.forename: F
authors.initials: F
authors.lastname: Morikawa
authors.forename: Y
authors.initials: Y
authors.lastname: Kon
mesh_terms: D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism / Q000528:radiation effects; D004578:Electron Spin Resonance Spectroscopy; D006801:Humans; D015227:Lipid Peroxidation / Q000528:radiation effects; D008544:Melanocytes / Q000378:metabolism / Q000528:radiation effects*; D008546:Melanoma, Experimental / Q000378:metabolism; D008560:Membrane Fluidity / Q000528:radiation effects*; D008563:Membrane Lipids / Q000528:radiation effects; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays
chemical_list: D001095:Arachidonic Acids; D008563:Membrane Lipids; D016718:Arachidonic Acid