Mutations in a protein kinase C homolog confer phorbol ester resistance on Caenorhabditis elegans.
The tpa-1 gene mediates the action of tumor-promoting phorbol esters in the nematode Caenorhabditis elegans. A genomic fragment that constitutes a portion of the tpa-1 gene was cloned by Tc1 transposon tagging and was used as a probe to screen a nematode complementary DNA library. One of the isolated complementary DNA clones had a nucleotide sequence that predicts a polypeptide of 526 amino acids. The predicted amino acid sequence revealed that the predicted tpa-1 protein sequence is highly similar to protein kinase C molecules from various animals, including man.
Tabuse
Y
Y
Fundamental Research Laboratories, NEC Corporation, Kawasaki, Kanagawa, Japan.
Nishiwaki
K
K
Miwa
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002107:Caenorhabditis / Q000187:drug effects* / Q000235:genetics; D003001:Cloning, Molecular; D003062:Codon; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D004351:Drug Resistance / Q000235:genetics; D005819:Genetic Markers; D008969:Molecular Sequence Data; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010703:Phorbol Esters / Q000494:pharmacology*; D011493:Protein Kinase C / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D003062:Codon; D005819:Genetic Markers; D010703:Phorbol Esters; D004247:DNA; D011493:Protein Kinase C; D004262:DNA Restriction Enzymes