An approach to the unification of suppressor T cell circuits: a simplified assay for the induction of suppression by T cell-derived, antigen-binding molecules (T-ABM).
A system is presented in which the in vitro response to sheep red blood cells (SRBC) can be regulated using antigenic determinants coupled to SRBC and T cell-derived antigen-binding molecules (T-ABM) directed against the coupled determinants. T suppressor-inducer factors (TsiF's) are composed of two molecules, one of which is a T-ABM and one which bears I-J determinants (I-J+ molecule). Using two purified T-ABM which have not previously been shown to have in vitro activity, we produced antigen-specific TsiF's which were capable of inducing the suppression of the anti-SRBC response. Suppression was found to require both the T-ABM and the I-J+ molecule, SRBC conjugated with the antigen for which the T-ABM was specific, and a population of Ly-2+ T cells in the culture. Two monoclonal TsiF (or TsF1) were demonstrated to induce suppression of the anti-SRBC response in this system, provided the relevant antigen was coupled to the SRBC in culture. The results are discussed in terms of the general functions of T-ABM in the immune system. This model will be useful in direct, experimental comparisons of the function of T-ABM and suppressor T cell factors under study in different systems and laboratories.
Chue
B
B
Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06510.
Ferguson
T A
TA
Beaman
K D
KD
Rosenman
S J
SJ
Cone
R E
RE
Flood
P M
PM
Green
D R
DR
D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism; D004912:Erythrocytes / Q000276:immunology; D006825:Hybridomas / Q000276:immunology; D007108:Immune Tolerance; D051379:Mice; D008810:Mice, Inbred C57BL / Q000276:immunology; D011485:Protein Binding; D012756:Sheep; D013491:Suppressor Factors, Immunologic / Q000276:immunology* / Q000378:metabolism; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D000954:Antigens, Surface; D013491:Suppressor Factors, Immunologic