[Coprecipitation of proteins and DNA-protein complexes with magnesium sarcolysate: research on an association of topoisomerase I and RNA polymerase with DNA].
A specific cosedimentation of proteins and their complexes with DNA at low temperature (M-band technique) has been demonstrated. Model experiments with reconstituted SV40 DNA-topoisomerase I and SV40 DNA-E. coli RNA polymerase complexes demonstrated the potential and capacities of the method. It allows fractionation of DNA-protein complexes from naked DNA and has greater range, higher reproducibility and lower background values than similar methods.
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D001426:Bacterial Proteins / Q000302:isolation & purification*; D011232:Chemical Precipitation; D003300:Copper / Q000633:toxicity; D004264:DNA Topoisomerases, Type I / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004279:DNA, Viral / Q000302:isolation & purification*; D012321:DNA-Directed RNA Polymerases / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology; D006878:Hydroxides / Q000633:toxicity; D007202:Indicators and Reagents; D008558:Melphalan / Q000494:pharmacology*; D008722:Methods; D013539:Simian virus 40 / Q000201:enzymology; D013696:Temperature; D014764:Viral Proteins / Q000302:isolation & purification*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004279:DNA, Viral; D006878:Hydroxides; D007202:Indicators and Reagents; D014764:Viral Proteins; C001606:cupric hydroxide; D003300:Copper; D012321:DNA-Directed RNA Polymerases; D004264:DNA Topoisomerases, Type I; D008558:Melphalan
Soosazhdenie belkov i kompleksov DNK--belok s sarkozilatom magniia: issledovanie assotsiatsii topoizomerazy I i RNK polimerazy s DNK.