Expression of EBNA-3 family in fresh B lymphocytes infected with Epstein-Barr virus.
Four transcripts belonging to the EBNA-3 family were detected during immortalization of human tonsillar lymphocytes with the Epstein-Barr virus. These four transcripts were also detected in immortalized B-lymphoblastoid cells (LCL) and the Burkitt's lymphoma cell line, Namalwa. A 5.0-kb transcript hybridized with the EBNA-3A probe; a 8.5-kb transcript hybridized with the EBNA-3B and -3C probes; and a 5.6- and a 3.6-kb transcript hybridized with the EBNA-3C probe. Sequencing and cDNA analysis showed that the EBNA-3C transcripts contained exons of the most rightward BamHI-E open reading frames, BERF-3 and BERF-4. Moreover, sequencing of cDNA clones revealed exons in the 5' portion homologous to regions in EBV BamHI-C, -W, and -U. Hybridization experiments with EBV BamHI-W and -U probes identified the 8.5- and 5.6-kb transcripts but not the 3.6-kb transcript in LCL. Organization of these transcripts from the EBNA-3 family is discussed.
Sawada
K
K
Department of Virology, Showa University Research Institute, St. Petersburg, Florida 33716.
Yamamoto
M
M
Tabata
T
T
Smith
M
M
Tanaka
A
A
Nonoyama
M
M
D000956:Antigens, Viral / Q000096:biosynthesis* / Q000235:genetics; D001402:B-Lymphocytes / Q000276:immunology*; D001483:Base Sequence; D015152:Blotting, Northern; D002051:Burkitt Lymphoma; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D003001:Cloning, Molecular; D015342:DNA Probes; D004279:DNA, Viral / Q000235:genetics; D019309:Epstein-Barr Virus Nuclear Antigens; D005091:Exons; D005786:Gene Expression Regulation; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012326:RNA Splicing; D012333:RNA, Messenger / Q000032:analysis; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D000956:Antigens, Viral; D015342:DNA Probes; D004279:DNA, Viral; D019309:Epstein-Barr Virus Nuclear Antigens; D012333:RNA, Messenger