[Macrophage activation and lymphokine production in the mouse by lymphocytes sensitized against Trichomonas vaginalis].
In this study we have examined whether resident peritoneal macrophages could be activated in vitro by immune T lymphocytes obtained from mice which were immunized with live Trichomonas vaginalis (T. vaginalis). T lymphocytes obtained from mice previously sensitized against T. vaginalis, showed a significant proliferative response when cultured in vitro in the presence of T. vaginalis. Resident peritoneal macrophages obtained from untreated syngenic Balb/c mice revealed an increasing cytotoxic activity against the protozoa when seeded with increasing concentrations of purified immune T lymphocytes and a constant number of H3 thymidine-labeled T. vaginalis. This cytotoxicity was detectable after 24 h of culture and peaked after 48 h. Supernatants obtained from cocultures of macrophages, immune T lymphocytes and T. vaginalis contained gamma-Interferon and TNF alpha/beta.
Martinotti
M G
MG
Istituto di Microbiologia, Università di Torino.
Jemma
C
C
Musso
T
T
Giovarelli
M
M
D000818:Animals; D002450:Cell Communication; D003602:Cytotoxicity, Immunologic; D007114:Immunization; D007371:Interferon-gamma / Q000378:metabolism*; D008213:Lymphocyte Activation; D008262:Macrophage Activation; D008264:Macrophages / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D010529:Peritoneal Cavity / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology*; D014246:Trichomonas vaginalis / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma
Attivazione macrofagica e produzione di linfochine nel topo da parte di linfociti sensibilizzati verso Trichomonas vaginalis.