title: Neurogenesis of the peripheral nervous system in Drosophila embryos: DNA replication patterns and cell lineages.
abstract: Cell lineages that give rise to the PNS were studied using the thymidine analog 5-bromo-2'-deoxyuridine (BrdU) to visualized DNA replication immunocytochemically. The precursors of the PNS in the body segments of Drosophila embryos replicate their DNA in a spatially and temporally stereotyped pattern. The sequence of DNA replication within developing sensory organs suggests particular lineage relationships of the cells that constitute a sensory organ, i.e., neuron and associated support cells. In embryos that are mutant for the achaete-scute complex or daughterless, in which most or all of the PNS is missing, no BrdU-labeled cells were found in the appropriate regions, suggesting that these PNS precursors either do not form or fail to replicate. Thus, the BrdU technique allows determination as to whether a mutation affects the PNS precursors or terminal differentiation.
authors.affiliation: Howard Hughes Medical Institute, Department of Physiology, University of California, San Francisco 94143.
authors.forename: R
authors.initials: R
authors.lastname: Bodmer
authors.forename: R
authors.initials: R
authors.lastname: Carretto
authors.forename: Y N
authors.initials: YN
authors.lastname: Jan
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication; D004331:Drosophila melanogaster / Q000196:embryology* / Q000235:genetics / Q000378:metabolism; D004475:Ectoderm / Q000166:cytology / Q000378:metabolism; D007150:Immunohistochemistry; D009154:Mutation; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D010525:Peripheral Nerves / Q000166:cytology / Q000196:embryology
chemical_list: D004247:DNA; D001973:Bromodeoxyuridine