Characteristics of glycine-activated conductances in cultured medullary neurons from embryonic rat.
Characteristics of glycine-activated currents in 10- to 20-day-old neurons were studied using the gigaseal whole-cell technique. Glycine activated a Cl- conductance that was blocked by strychnine acting as a mixed inhibitor, effecting both Imax and Kd. Glycine receptors with at least two different sensitivities to strychnine were found, based on the apparent inhibition constants (Ki). These two Ki values were associated with different Hill coefficients for the glycine responses. Cs+ activated the same receptor and also activated a relatively nonselective cation conductance.
Lewis
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Department of Physiology, State University of New York, Buffalo 14214.
Ahmed
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Faber
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D000818:Animals; D002478:Cells, Cultured; D002586:Cesium / Q000494:pharmacology; D002712:Chlorides / Q000502:physiology*; D004622:Embryo, Mammalian; D005998:Glycine / Q000494:pharmacology*; D007700:Kinetics; D008526:Medulla Oblongata / Q000187:drug effects / Q000502:physiology*; D008564:Membrane Potentials; D051381:Rats; D011919:Rats, Inbred Strains; D018009:Receptors, Glycine; D017981:Receptors, Neurotransmitter / Q000187:drug effects / Q000502:physiology*; D013331:Strychnine / Q000494:pharmacology*
D002712:Chlorides; D018009:Receptors, Glycine; D017981:Receptors, Neurotransmitter; D002586:Cesium; D013331:Strychnine; D005998:Glycine