Functional immunoregulatory T-cell abnormalities in cystic fibrosis patients.
The role of B cells and regulatory T cells in the reduced in vitro IgG synthesis of cystic fibrosis (CF) patients was studied. Intact proportion, proliferation, and differentiation of B cells and reduced suppressor and helper T-cell function were found. To explore the T-cell defects further, CF sera or supernatant derived from Pseudomonas aeruginosa cultures (PA supernatant) was added to the relevant T helper- and suppressor-cell assays. Both CF sera derived from PA-positive patients and PA supernatant interfered with the appearance of interleukin 2 (IL-2) receptors and with the functional enhancement caused by exogenously added IL-2. PA-negative CF patients, however, also had functional T-cell defects and inhibitory sera, but these sera did not affect IL-2 pathways. Thus different serum factors and intrinsic T-cell defects in CF patients are suggested.
Lahat
N
N
Immunology Unit, Carmel Hospital, Haifa, Israel.
Rivlin
J
J
Iancu
T C
TC
D000293:Adolescent; D000328:Adult; D000921:Antibody-Producing Cells / Q000378:metabolism; D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes / Q000502:physiology; D002648:Child; D002675:Child, Preschool; D003208:Concanavalin A / Q000494:pharmacology; D003550:Cystic Fibrosis / Q000276:immunology*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D066298:In Vitro Techniques; D007223:Infant; D007376:Interleukin-2 / Q000494:pharmacology; D007378:Interleukins / Q000494:pharmacology; D008213:Lymphocyte Activation; D008297:Male; D010641:Phenotype; D011550:Pseudomonas aeruginosa / Q000276:immunology; D013601:T-Lymphocytes / Q000502:physiology*; D050378:T-Lymphocytes, Regulatory / Q000502:physiology
D000954:Antigens, Surface; D007074:Immunoglobulin G; D007376:Interleukin-2; D007378:Interleukins; D003208:Concanavalin A