Human rhinovirus serotype 2: in vitro synthesis of an infectious RNA.
A complete cDNA copy of human rhinovirus serotype 2 RNA was placed under the control of a T7 RNA polymerase promoter. An in vitro transcribed RNA containing two extra G residues at the 5' end gave rise to plaques on transfection into HeLa cells. The efficiency was approximately half that obtained with viral RNA. On the contrary, an in vitro synthesized RNA containing 16 additional nucleotides at the 5' end was not infectious. This ability to make an infectious in vitro transcribed RNA will be useful in studying the characteristics of viruses using the human rhinoviral minor group receptor.
Duechler
M
M
Institut für Biochemie, der Universitaet Wien, Vienna, Austria.
Skern
T
T
Blaas
D
D
Berger
B
B
Sommergruber
W
W
Kuechler
E
E
D003001:Cloning, Molecular; D006801:Humans; D010957:Plasmids; D012367:RNA, Viral / Q000096:biosynthesis*; D015183:Restriction Mapping; D012229:Rhinovirus / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
D012367:RNA, Viral