Genetic transformation of the mosquito Aedes aegypti by micro-injection of DNA.
We report the successful introduction of heterologous DNA sequences into embryos of the mosquito Aedes aegypti (L.) by microinjection. The injected DNA carried P transposable element sequences, derived from and known to facilitate transformation in Drosophila melanogaster. Two plasmids, one of which carried a dominant selectable marker, were introduced into the posterior of embryos prior to pole cell formation and subsequently taken up into the germ line of transformed individuals. Stable transfer of the selectable marker (G418 resistance) was demonstrated over two generations. The precise nature of these putative P mediated integration events is currently being investigated. However, the results presented here establish the technique of DNA transformation for the genetic manipulation of Aedes aegypti.
Morris
A C
AC
Department of Medical Entomology, Liverpool School of Tropical Medicine.
Eggleston
P
P
Crampton
J M
JM
D000330:Aedes / Q000235:genetics*; D000818:Animals; D004247:DNA / Q000008:administration & dosage*; D005822:Genetic Vectors; D007303:Insect Vectors / Q000235:genetics*; D008845:Microinjections; D010957:Plasmids; D014170:Transformation, Genetic
D004247:DNA