Contributions of the beta-subunit to spectrin structure and function.
The three avian spectrins that have been characterized consist of a common alpha-subunit (240 kD) paired with an isoform-specific beta-subunit from either erythrocyte (220 or 230 kD), brain (235 kD), or intestinal brush border (260 kD). Analysis of avian spectrins, with their naturally occurring "subunit replacement" has proved useful in assessing the relative contribution of each subunit to spectrin function. In this study we have completed a survey of avian spectrin binding properties and present morphometric analysis of the relative flexibility and linearity of various avian and human spectrin isoforms. Evidence is presented that, like its mammalian counterpart, avian brain spectrin binds human erythroid ankyrin with low affinity. Cosedimentation analysis demonstrates that 1) avian erythroid protein 4.1 stimulates spectrin-actin binding of both mammalian and avian erythrocyte and brain spectrins, but not the TW 260/240 isoform, 2) calpactin I does not potentiate actin binding of either TW 260/240 or brain spectrin, and 3) erythrocyte adducin does not stimulate the interaction of TW 260/240 with actin. In addition, a morphometric analysis of rotary-shadow images of spectrin isoforms, individual subunits, and reconstituted complexes from isolated subunits was performed. This analysis revealed that the overall flexibility and linearity of a given spectrin heterodimer and tetramer is largely determined by the intrinsic rigidity and linearity of its beta-spectrin subunit. No additional rigidity appears to be imparted by noncovalent associations between the subunits. The scaled flexural rigidity of the most rigid spectrin analyzed (human brain) is similar to that reported for F-actin.
Coleman
T R
TR
Department of Biology, Yale University, New Haven, CT 06511-8112.
Fishkind
D J
DJ
Mooseker
M S
MS
Morrow
J S
JS
D000199:Actins / Q000378:metabolism; D000818:Animals; D017487:Ankyrins; D001798:Blood Proteins / Q000378:metabolism; D002498:Centrifugation; D002645:Chickens; D004912:Erythrocytes / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism; D008854:Microscopy, Electron; D008871:Microvilli; D011487:Protein Conformation; D013049:Spectrin / Q000302:isolation & purification / Q000502:physiology / Q000648:ultrastructure
D000199:Actins; D017487:Ankyrins; D001798:Blood Proteins; D008565:Membrane Proteins; D013049:Spectrin