title: Photoaffinity labelling of the oxytocin receptor in plasma membranes from rat mammary gland.
abstract: Plasma membranes from rat mammary gland containing a high concentration of [3H]oxytocin binding sites (2.8 pmol/mg protein) were used for photoaffinity labelling experiments. Competitive binding experiments show that these receptors bind with high affinity the specific oxytocin agonist [Thr4, Sar7]oxytocin and the analogue of 1-deamino-[8-lysine]vasopressin containing a photoreactive azidobenzoyl group (Abz) at the side chain of lysine. The tritium-labelled (50 Ci/mol) photoreactive analogue incorporated into a membrane protein with an apparent relative molecular mass of 65,000 +/- 3000 Da (n = 16). The labelling of this protein was completely suppressed by an excess of oxytocin.
authors.affiliation: Max-Planck-Institut für Biophysik, Frankfurt, FRG.
authors.forename: M
authors.initials: M
authors.lastname: Müller
authors.forename: M S
authors.initials: MS
authors.lastname: Soloff
authors.forename: F
authors.initials: F
authors.lastname: Fahrenholz
mesh_terms: D000345:Affinity Labels; D000818:Animals; D001667:Binding, Competitive; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D008321:Mammary Glands, Animal / Q000032:analysis / Q000378:metabolism*; D008970:Molecular Weight; D010121:Oxytocin / Q000032:analysis / Q000378:metabolism*; D010777:Photochemistry; D051381:Rats; D011945:Receptors, Angiotensin / Q000032:analysis / Q000378:metabolism*; D018045:Receptors, Oxytocin; D013329:Structure-Activity Relationship
chemical_list: D000345:Affinity Labels; D011945:Receptors, Angiotensin; D018045:Receptors, Oxytocin; D010121:Oxytocin