Induction of histidine decarboxylase in rat basophilic leukemia cells by interferon and prevention of its effect in coincubation with ADP-ribosyltransferase inhibitors.
Treatment of rat basophilic leukemia cell line (2H3) with interferon-alpha significantly increased intracellular histamine levels. On the other hand, the histidine content was decreased reciprocally by interferon in a dose-dependent manner. Concomitantly, the activity of histidine decarboxylase, the enzyme responsible for histamine synthesis, was augmented. The increase in histidine decarboxylase activity was partially abolished in co-incubation with inhibitors of ADP-ribosyltransferase, such as 3-aminobenzamide or nicotinamide. These results suggest the pivotal role of activation of histidine decarboxylase, presumably through ADP-ribosylation of the enzyme, in interferon-induced histamine synthesis.
Kito
T
T
Department of Pediatrics, Faculty of Medicine, Kyoto University, Japan.
Kubota
M
M
Takimoto
T
T
Akiyama
Y
Y
Heike
T
T
Mikawa
H
H
D000818:Animals; D001549:Benzamides / Q000494:pharmacology*; D002262:Carboxy-Lyases / Q000096:biosynthesis*; D002460:Cell Line; D004790:Enzyme Induction; D006640:Histidine Decarboxylase / Q000096:biosynthesis*; D007370:Interferon Type I / Q000494:pharmacology*; D007700:Kinetics; D015471:Leukemia, Basophilic, Acute / Q000201:enzymology*; D009536:Niacinamide / Q000494:pharmacology*; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D051381:Rats
D001549:Benzamides; D007370:Interferon Type I; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D009536:Niacinamide; C025160:3-aminobenzamide; D002262:Carboxy-Lyases; D006640:Histidine Decarboxylase