title: Functional analysis of point mutations in the AAUAAA motif of the SV40 late polyadenylation signal.
abstract: We have constructed 14 independent point mutations in the conserved AAUAAA element of the SV40 late polyadenylation signal in order to study the recognition and function of alternative polyadenylation signals. A variant RNA containing an AUUAAA was polyadenylated at 20% the level of wild-type substrate RNA, while all other derivatives tested were not functional in vitro. The AUUAAA variant RNA formed specific complexes in native polyacrylamide gels and crosslinked to the AAUAAA-specific 64kd polypeptide, but at a lower efficiency than wild-type substrate RNA.
authors.affiliation: Princeton University, Department of Biology, NJ 08544.
authors.forename: J
authors.initials: J
authors.lastname: Wilusz
authors.forename: S M
authors.initials: SM
authors.lastname: Pettine
authors.forename: T
authors.initials: T
authors.lastname: Shenk
mesh_terms: D001483:Base Sequence; D014644:Genetic Variation; D008969:Molecular Sequence Data; D009154:Mutation; D011061:Poly A / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D013539:Simian virus 40 / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D012367:RNA, Viral; D011061:Poly A