An Epstein-Barr virus-transformed B cell line produces autoregulatory interleukin-1 that regulates bone remodeling.
In this study, we demonstrate that an Epstein-Barr virus-transformed B cell line, A-11, produced interleukin-1 (IL-1), a cytokine that regulates bone remodeling. A-11 cells produce IL-1 in a cell dose- and culture time-related manner. The IL-1 activity was neutralized by recombinant human IL-1 (rhIL-1) alpha antiserum, but not by rhIL-1 beta antiserum. The IL-1 was semi-purified by (NH4)2SO4 precipitation, Superose prep 12 gel filtration, and anion-exchange chromatography strongly stimulated in vitro bone resorption. The stimulatory effect of the purified IL-1 on bone resorption was prostaglandin independent. Purified IL-1 inhibited DNA and collagen synthesis in the osteoblastic cell line MC3T3-E1. However, it enhanced significantly the cellular activity of alkaline phosphatase (EC 3.1.3.1), a marker enzyme for differentiation of osteoblasts. On the other hand, A-11 cell proliferation was inhibited by addition of rhIL-1 alpha antiserum, but not by rhIL-1 beta antiserum. And cell proliferation was stimulated by exogenous rhIL-1 alpha and -beta.
Hanazawa
S
S
Department of Oral Microbiology, School of Dentistry, Meikai University, Saitama, Japan.
Amano
S
S
Hamano
A
A
Katoh
H
H
Honda
T
T
Takada
M
M
Hirose
S
S
Kitano
S
S
D000469:Alkaline Phosphatase / Q000378:metabolism; D000645:Ammonium Sulfate; D001402:B-Lymphocytes / Q000378:metabolism*; D001862:Bone Resorption; D002455:Cell Division; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D011232:Chemical Precipitation; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003094:Collagen / Q000096:biosynthesis; D004247:DNA / Q000096:biosynthesis; D004854:Herpesvirus 4, Human; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D010006:Osteoblasts / Q000378:metabolism; D011994:Recombinant Proteins
D007106:Immune Sera; D007375:Interleukin-1; D011994:Recombinant Proteins; D003094:Collagen; D004247:DNA; D000469:Alkaline Phosphatase; D000645:Ammonium Sulfate