[Effect of the method on the results of determining T-cell subpopulations].
Two methods of determining lymphocyte subpopulations were compared on samples obtained from 18 healthy blood donors and 16 HIV-positive patients. In the whole-blood method directly marked monoclonal antibodies were used, whereas Ficoll-separated mononuclear cells were assayed indirectly. The number of CD4-positive helper cells was significantly lower in both groups in the whole-blood test, but that of CD8-positive suppressor cells significantly increased after Ficoll separation. In HIV-infected patients the values for CD8-positive lymphocytes were even further apart than in the healthy donors. As a result the difference in the ratio CD4/CD8 was higher in HIV-infected patients (0.3 vs 0.65; P less than 0.001) than in the healthy donors (1.35 vs 1.8; P less than 0.001).
Neubauer
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A
Abteilung Innere Medizin und Poliklinik, Universitätsklinikum Rudolf Virchow, Standort Charlottenburg, Berlin.
van Lessen
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A
Huhn
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D
D000906:Antibodies / Q000032:analysis; D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D001782:Blood Donors; D016827:CD8 Antigens; D002469:Cell Separation; D005069:Evaluation Studies as Topic; D005362:Ficoll; D005434:Flow Cytometry; D006679:HIV Seropositivity / Q000097:blood / Q000276:immunology; D006801:Humans; D007958:Leukocyte Count / Q000379:methods; D011446:Prospective Studies; D013601:T-Lymphocytes / Q000145:classification* / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000145:classification / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000145:classification / Q000276:immunology
D000906:Antibodies; D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D016827:CD8 Antigens; D005362:Ficoll
Einfluss der Methode auf das Ergebnis der Bestimmung von T-Zellsubpopulationen.