[Evaluation of the laser-Doppler velocimetry method in the measurement of gastric blood flow in rats--spatial resolution].
In this paper, gastric blood flow in rats was measured with the laser-Doppler velocimetry method (the LDV method) to study about the tissue locus where its flow signal arises (spatial resolution). In the measurement throughout some 1 mm thickness of another nonperfused gastric wall interposed between the laser probe and gastric mucosal surface, its laser flow signal was 17% of the flow signal in the usual measurement. In the blood flow measurement with the LDV prove on the mucosal and the serosal surface of gastric wall, the laser flow signal on the mucosal surface was higher (p less than 0.05) than that on the serosal surface. These results suggested that the laser flow signal mainly arose from the tissue right under the laser probe, reflecting the total gastric blood flow of the gastric wall. In the regional blood flow measurement at corpus and antrum, the ratio between antral and corpus flow signals by the LDV method was similar to that between gastric mucosal blood flows at both sites by the hydrogen gas clearance technique. In the blood flow measurement after the intravenous infusion of each pentagastrin, isoproterenol, and vasopressin, flow signal of the LDV method could detect the each effect of these drugs on gastric mucosal blood flow as well as well as the hydrogen gas clearance technique. These results showed that the laser flow signal and gastric mucosal blood flow were mutually correlated.(ABSTRACT TRUNCATED AT 250 WORDS)
Saita
H
H
Murakami
M
M
Mizumo
M
M
Yoo
J K
JK
Ashida
Y
Y
Inoue
R
R
Inada
M
M
Kita
T
T
Miyake
T
T
D000818:Animals; D005753:Gastric Mucosa / Q000098:blood supply; D006859:Hydrogen / Q000032:analysis; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012039:Regional Blood Flow; D012212:Rheology; D013270:Stomach / Q000098:blood supply*
D006859:Hydrogen