Calcium chelators induce association with the detergent-insoluble cytoskeleton and functional inactivation of the transferrin receptor in reticulocytes.
Incubation of reticulocytes with EDTA, EGTA (ethylene glycol bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid) and BAPTA (1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid), but not with desferrioxamine B, at temperatures above 20 degrees C resulted in the loss of their ability to take up iron in a temperature-, time- and concentration-dependent manner. No inhibition of transferrin or iron uptake occurred if the incubations were performed at 20 degrees C or below. At higher temperatures, the inhibition was attributable to loss of functional transferrin receptors, not to altered affinity or endocytosis of the remaining receptors. The changes could not be reversed by washing the cells and reincubation in the presence of Ca2+, Mg2+ or Zn2+. However, they could be completely prevented by performing the initial incubation with chelators in the presence of diferric transferrin and partly prevented by the use of apotransferrin. Incubation with the chelators resulted in much less reduction in the ability of the cells to bind anti-transferrin receptor immunoglobulin than transferrin. The fate of the receptor was studied by polyacrylamide gel electrophoresis of reticulocyte membrane proteins before and after extraction with Triton X-100, and by immunological staining of Western blots for the transferrin receptor. Treatment of the cells with EDTA led to a loss of the ability of Triton X-100 to solubilize the receptor and its retention in the Triton-insoluble cytoskeletal matrix of the cells. It is concluded that incubation of reticulocytes with the chelators at temperatures above 20 degrees C causes an altered interaction of the transferrin receptor with the cytoskeleton. This change, which is probably due to chelation of Ca2+ in the cell membrane, is accompanied by an irreversible loss of the receptor's ability to bind transferrin.
Morgan
E H
EH
Department of Physiology, University of Western Australia, Nedlands.
D000818:Animals; D015220:Calcium Channels / Q000187:drug effects* / Q000502:physiology; D002463:Cell Membrane Permeability / Q000187:drug effects; D002614:Chelating Agents / Q000494:pharmacology*; D003599:Cytoskeleton / Q000187:drug effects* / Q000502:physiology; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology / Q000031:analogs & derivatives; D004591:Electrophoresis, Polyacrylamide Gel; D004705:Endocytosis / Q000187:drug effects; D005290:Ferric Compounds / Q000494:pharmacology; D007106:Immune Sera; D007501:Iron / Q000097:blood; D008565:Membrane Proteins / Q000302:isolation & purification; D017830:Octoxynol; D011092:Polyethylene Glycols; D011817:Rabbits; D051381:Rats; D011990:Receptors, Transferrin / Q000187:drug effects* / Q000276:immunology / Q000502:physiology; D012156:Reticulocytes / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D012995:Solubility; D013329:Structure-Activity Relationship; D014168:Transferrin / Q000378:metabolism
D015220:Calcium Channels; D002614:Chelating Agents; D005290:Ferric Compounds; D007106:Immune Sera; D008565:Membrane Proteins; D011990:Receptors, Transferrin; D014168:Transferrin; D011092:Polyethylene Glycols; D004533:Egtazic Acid; D017830:Octoxynol; D004492:Edetic Acid; D007501:Iron; C025603:1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid