Changes in nuclear inositol phospholipids induced in intact cells by insulin-like growth factor I.
Swiss 3T3 cells were labelled for 36 hours with high levels of 3H-myo-inositol and the radioactivity in nuclear inositol phospholipids was measured. Treatment of cells for 2 minutes, but not for 4 hours, with mitogenic concentrations of insulin-like growth factor I and bombesin caused a slight decrease in PtdIns and more marked decreases in PtdInsP and PtdInsP2. These effects were not seen if isolated nuclei were incubated with IGF-I and bombesin. We interpret these results to mean that rapid mass changes occur in nuclear inositol phospholipids in the early stages of the mitotic response.
Cocco
L
L
Istituto di Morfologia Umana Normale, University of Chieti, Italy.
Martelli
A M
AM
Gilmour
R S
RS
Ognibene
A
A
Manzoli
F A
FA
Irvine
R F
RF
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D007295:Inositol Phosphates / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D051379:Mice; D010716:Phosphatidylinositols / Q000378:metabolism*; D013002:Somatomedins / Q000494:pharmacology*
D007295:Inositol Phosphates; D010716:Phosphatidylinositols; D013002:Somatomedins; D007334:Insulin-Like Growth Factor I