Quantitation of putative glycoprotein X in bioengineered pseudorabies vaccine virus culture medium by ELISA.
An enzyme-linked immunosorbent assay has been developed for the detection and quantitation of putative pseudorabies glycoprotein X (gX) in bulk bioengineered PRV delta gX delta tk-1 pseudorabies vaccine virus culture medium supernatants. The assay has a dynamic range of 0.2-25 ng, with a best linear region of 0.4-12.5 ng (correlation coefficient = 0.99) which permits 1 ppm discrimination for gX.
Eaton
L C
LC
Control Biotechnology Analytical Assay Development, Upjohn Company, Kalamazoo, MI 49001.
D000956:Antigens, Viral / Q000032:analysis*; D003470:Culture Media / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D014612:Vaccines / Q000032:analysis*; D014614:Vaccines, Synthetic / Q000032:analysis*; D014759:Viral Envelope Proteins / Q000032:analysis*; D014765:Viral Vaccines / Q000032:analysis*
D000956:Antigens, Viral; D003470:Culture Media; D014612:Vaccines; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C042966:pseudorabies virus glycoproteins