Removal of troponin C from natural actomyosin of rabbit skeletal muscle by CDTA.
CDTA-treatment of rabbit skeletal natural actomyosin resulted in the complete removal of troponin C and the abolishment of Ca2+-activated ATPase activity. The addition of troponin C restored the Ca2+-activated ATPase activity to almost the same extent as that of the intact natural actomyosin. These finding are consistent with those of previous studies on the skeletal and cardiac myofibrils.
Shiraishi
F
F
Ohtsuki
I
I
D000818:Animals; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D004492:Edetic Acid / Q000031:analogs & derivatives*; D004586:Electrophoresis; D009132:Muscles / Q000032:analysis*; D009218:Myosins / Q000032:analysis*; D011817:Rabbits; D014336:Troponin / Q000302:isolation & purification*; D019209:Troponin C
D014336:Troponin; D019209:Troponin C; C005390:CDTA; D004492:Edetic Acid; D009218:Myosins; D000252:Calcium-Transporting ATPases