title: [The search for microorganisms sensitive to deoxynivalenol, zearalenone and aflatoxin B1].
abstract: Sensitivity to T-2 toxin, deoxynivalenol (DOV), zearalenon (ZL) and aflatoxin (AT) B1 was comparatively studied in yeast strains Saccharomyces fragilis 25 D, Candida pseudotropicalis 44 nk and Saccharomyces lactis BKMY-459, sensitive to T-2 toxin, and strain Bacillus megaterium BKMB-44, sensitive to toxic metabolites of Aspergillus species of fungi. Minimum amounts of T-2 toxin inducing on Silufol plates sites of the yeast strain growth suppression, comprised 20-50 ng, maximum sensitivity was recorded in Saccharomyces lactis BKMY-459. Minimum content of DOV suppressing the yeast growth comprised 10-50 micrograms, and that of ZL 10-25 micrograms. Bacillus megaterium BKMB-44 culture proved to be highly sensitive to AT B1, 1 microgram of which suppressed the strain growth. This culture was resistant to T-2 toxin and DOV in doses of 1 microgram and 200 micrograms, respectively. ZL suppressed Bacillus megaterium growth when its minimum content in the plaque was 0.5 = 1 microgram. The authors have considered the possibility of using the microorganism strains studied for screening toxic grain lots and toxigenic fungus strains.
authors.forename: I B
authors.initials: IB
authors.lastname: Kuvaeva
authors.forename: E V
authors.initials: EV
authors.lastname: Boltianskaia
authors.forename: E A
authors.initials: EA
authors.lastname: Kroiakova
mesh_terms: D016604:Aflatoxin B1; D000348:Aflatoxins / Q000494:pharmacology*; D001230:Aspergillus / Q000187:drug effects; D001407:Bacillus / Q000187:drug effects; D005516:Food Microbiology; D008826:Microbial Sensitivity Tests; D012118:Resorcinols / Q000494:pharmacology*; D012440:Saccharomyces / Q000187:drug effects; D012717:Sesquiterpenes / Q000494:pharmacology*; D014255:Trichothecenes / Q000494:pharmacology*; D015025:Zearalenone / Q000494:pharmacology*
chemical_list: D000348:Aflatoxins; D012118:Resorcinols; D012717:Sesquiterpenes; D014255:Trichothecenes; D015025:Zearalenone; D016604:Aflatoxin B1; C007262:deoxynivalenol
vernacular_title: Poisk mikroorganizmov, chuvstvitel'nykh k dezoksinivalenolu, zearalenonu i aflatoksinu B1.