Wide distribution of homologs of Escherichia coli Kdp K+-ATPase among gram-negative bacteria.
We used Southern blotting to screen a variety of bacterial genes for homology to the kdp genes of Escherichia coli, genes that encode an ATP-driven K+ transport system. We found that most enterobacteria have sequences homologous to those of the three kdp structural genes and the kdpD regulatory gene. A number of distantly related species, including some cyanobacteria, have sequences homologous to those of the structural genes but not the regulatory gene. In all cases only a single region of homology was found. These results suggest that ATP-driven transport systems similar to the Kdp system in structure and regulation are found in many enteric organisms. In other gram-negative organisms, the ATPase is more divergent, retaining good homology at the DNA level only to the highly conserved phosphorylated subunit of the ATPase.
Walderhaug
M O
MO
Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.
Litwack
E D
ED
Epstein
W
W
D000251:Adenosine Triphosphatases / Q000235:genetics*; D001483:Base Sequence; D027682:Cation Transport Proteins; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005796:Genes; D005798:Genes, Bacterial; D006090:Gram-Negative Bacteria / Q000201:enzymology / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D027682:Cation Transport Proteins; D000251:Adenosine Triphosphatases; C022761:potassium transporting ATPase