Semiconservative and unscheduled DNA synthesis on mammalian cells and its modification by glyoxylic compounds.
The interaction of glyoxal and four other glyoxylic compounds with semiconservative DNA synthesis and with unscheduled DNA synthesis induced by 25-G X-rays on TC-SV40 hamster cells has been studied. Both syntheses were evaluated by measuring the incorporation of [3H-methyl]-thymidine into the newly synthetized DNA. The unscheduled DNA synthesis amounts to 4% of semiconservative synthesis. The modification of both syntheses by the glyoxylic compounds was tested using the products at non-toxic concentrations for the cells. All the glyoxals inhibited semiconservative DNA synthesis and potentiated unscheduled DNA synthesis at rather similar levels. These effects have been compared with the radiosensitizing activity of these glyoxals in the same TC-SV40 cells and no relationship could be established.
Cornago
P
P
Instituto Química Fisica Rocasolano CSIC, Madrid, Spain.
Lopez Zumel
M C
MC
Santos
L
L
Pintado
M
M
D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D006224:Cricetinae; D004247:DNA / Q000096:biosynthesis* / Q000187:drug effects / Q000528:radiation effects; D004261:DNA Replication / Q000187:drug effects* / Q000528:radiation effects; D006037:Glyoxal / Q000494:pharmacology*; D006038:Glyoxylates / Q000494:pharmacology*; D007700:Kinetics; D008333:Mandelic Acids; D014407:Tumor Cells, Cultured; D014965:X-Rays
D000447:Aldehydes; D006038:Glyoxylates; D008333:Mandelic Acids; C012482:phenylglyoxylic acid; D006037:Glyoxal; D004247:DNA; C031150:glyoxylic acid