Photodynamic inactivation of simian immunodeficiency virus.
A photodynamic flow system employing a dihematoporphyrin ether (DHE) was tested for its ability to inactivate the in vitro infectivity of simian immunodeficiency virus (SICMac) at 630 +/- 5 nm with a light fluence of 5 J/cm2. Cell-free SIVMac was inactivated by photoactivated hematoporphyrin derivative in a dose-dependent fashion. Since SIVMac is closely related to human immunodeficiency virus type 2 (HIV-2) and we have previously reported the successful photodynamic inactivation of HIV-1 in cell-free medium as well as in whole human blood, this technology has the potential for the eradication of transfusion-associated acquired immunodeficiency diseases caused by the above-mentioned retroviruses.
Chanh
T C
TC
Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, TX 78284.
Allan
J S
JS
Matthews
J L
JL
Sogandares-Bernal
F
F
Judy
M M
MM
Skiles
H
H
Leveson
J
J
Marengo-Rowe
A
A
Newman
J T
JT
D000998:Antiviral Agents / Q000494:pharmacology* / Q000528:radiation effects; D002478:Cells, Cultured; D017323:Dihematoporphyrin Ether; D006415:Hematoporphyrins / Q000494:pharmacology* / Q000528:radiation effects; D006801:Humans; D008027:Light; D008214:Lymphocytes; D010777:Photochemistry; D015302:Simian Immunodeficiency Virus / Q000187:drug effects* / Q000502:physiology; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D006415:Hematoporphyrins; D017323:Dihematoporphyrin Ether