Gas chromatographic determination of nicotinamide in meats and meat products as 3-cyanopyridine.
The determination of nicotinamide as 3-cyanopyridine after dehydration by heptafluorobutyric anhydride (HFB) was performed by gas-liquid chromatography (GLC) with flame ionization detection (GLC-FID) and a column of 5% OV-17 on Chromosorb W AW DMCS at 130 degrees C. Determination was possible with 3-100 micrograms of the dehydrated reaction mixture. The procedure for determining nicotinamide in various meats and meat products involves direct analysis by GLC-FID without a clean-up stage; the detection limit is 5 ppm and the recovery ranged from 93.4 to 104.6% (average 98.0%). Various possible interferents in the samples did not interfere in the production or determination of 3-cyanopyridine. The procedure is suitable for routine use. The dehydrated derivative of nicotinamide was confirmed as 3-cyanopyridine by combined gas chromatography-mass spectrometry and infrared spectrometry.
Tanaka
A
A
Saitama Institute of Public Health, Japan.
Iijima
M
M
Kikuchi
Y
Y
Hoshino
Y
Y
Nose
N
N
D002849:Chromatography, Gas; D003890:Desiccation; D005410:Flame Ionization; D008460:Meat / Q000032:analysis*; D008461:Meat Products / Q000032:analysis*; D009536:Niacinamide / Q000032:analysis*; D011725:Pyridines / Q000032:analysis*
D011725:Pyridines; D009536:Niacinamide; C020729:3-cyanopyridine