[Effect of androgen on the onset of diabetes in the KK mice treated with monosodium aspartate].
Obese diabetes was induced by monosodium aspartate (MSA) administration in KK male mice and the diabetic KK mice were divided into two groups, younger (12-week-old) and older (35-week-old). The diabetic KK mice were castrated and administered with androgen and effect of androgen on glycosuria appearance was investigated. Androgen dependent tear proteins (Mtp-M) were detected by the method of polyacrylamide gel electrophoresis. Blood androgen level was estimated by observation of change of the pattern of Mtp-M. In the younger mice group, glycosuria disappeared temporarily after castration and then appeared naturally again. The Mtp-M declined with castration, but did not disappear in this experimental period. In the older mice group, glycosuria and Mtp-M disappeared completely and blood glucose level decreased considerably after castration. However, in the castrated older mice, the glycosuria and the Mtp-M appeared again after the administration of dehydroepiandrosterone (DHEA), and the increasing of blood glucose level was observed. These results strongly suggested that androgen had an important role in the onset of diabetes in the KK mice treated with MSA.
Higuchi
N
N
Department of Veterinary Biochemistry, Nippon Veterinary and Zootechnical College, Tokyo, Japan.
Sasaki
M
M
Arai
T
T
Oki
Y
Y
D000367:Age Factors; D000728:Androgens / Q000097:blood / Q000502:physiology*; D000818:Animals; D001224:Aspartic Acid; D001786:Blood Glucose / Q000032:analysis; D002369:Castration; D003687:Dehydroepiandrosterone / Q000494:pharmacology; D003920:Diabetes Mellitus / Q000097:blood / Q000139:chemically induced*; D003921:Diabetes Mellitus, Experimental / Q000097:blood*; D005136:Eye Proteins / Q000097:blood; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009765:Obesity
D000728:Androgens; D001786:Blood Glucose; D005136:Eye Proteins; C042434:tear proteins; D001224:Aspartic Acid; D003687:Dehydroepiandrosterone