The GABA/benzodiazepine receptor complex in the central amygdalar nucleus and stress ulcers in rats.
The effects of bilateral microinjections of chlordiazepoxide and GABA into the central amygdalar nucleus on gastric ulcer formation induced by cold-restraint were examined in chronically implanted Wistar rats. Higher doses of chlordiazepoxide (20 and 30 micrograms/amygdala) significantly reduced stress ulcer development, whereas a lower dose (2.5 micrograms) produced a nonsignificant increase in ulcer severity. A similar dose/response pattern was observed following GABA administration. The benzodiazepine receptor antagonist Ro15-1788, applied to the amygdala, abolished the protective effects of both chlordiazepoxide and GABA. In addition, when Ro15-1788 (10 micrograms) was injected into the amygdala by itself, it aggravated the gastric stress pathology. However, a lower dose (5 micrograms) had an attenuating effect, opposite to the pattern of effects produced by chlordiazepoxide and GABA. The role of the amygdalar GABA-benzodiazepine receptor complex in stressful conditions is discussed.
Sullivan
R M
RM
St. Francis Xavier University, Antigonish, Nova Scotia, Canada.
Henke
P G
PG
Ray
A
A
Hebert
M A
MA
Trimper
J M
JM
D000679:Amygdala / Q000187:drug effects / Q000503:physiopathology*; D000818:Animals; D001143:Arousal / Q000502:physiology*; D002707:Chlordiazepoxide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005442:Flumazenil / Q000494:pharmacology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011963:Receptors, GABA-A / Q000187:drug effects / Q000502:physiology*; D013276:Stomach Ulcer / Q000503:physiopathology*; D013315:Stress, Psychological / Q000150:complications*
D011963:Receptors, GABA-A; D005442:Flumazenil; D002707:Chlordiazepoxide