Effects of the supernatants of mixed lymphocyte cultures and decidual cell line cultures on mouse embryo development in vitro.
The effects of supernatants of human mixed lymphocyte cultures (MLC), with or without human decidual cell line culture extract (decidual factor; DCF), on F1-hybrid mouse embryo development in vitro from the two-cell stage were investigated. The development of mouse embryos from the two-cell stage through the expanded blastocyst stage was facilitated significantly by the addition of supernatants of not only MLC, but also MLC supplemented with DCF (MLCDCF) to the culture medium. Moreover, the supernatant of MLCDCF accelerated the attachment of the hatched blastocyst to the culture dish substratum and the outgrowth of trophoblasts in vitro. The findings indicate that the supernatant of MLCDCF facilitates the in vitro activity of mouse embryos for implantation and that the maternal immune response, along with the decidual tissue, contributes to the implantation processes.
Fukuda
A
A
Department of Gynecology & Obstetrics, Faculty of Medicine, Kyoto University, Japan.
Mori
T
T
Mori
E
E
Tatsumi
K
K
Kanzaki
H
H
Mori
T
T
D000818:Animals; D001755:Blastocyst / Q000276:immunology / Q000502:physiology*; D002457:Cell Extracts / Q000276:immunology; D002460:Cell Line; D002970:Cleavage Stage, Ovum / Q000276:immunology / Q000502:physiology*; D003470:Culture Media; D003656:Decidua / Q000276:immunology*; D005260:Female; D006801:Humans; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice
D002457:Cell Extracts; D003470:Culture Media