Aphidicolin resistance in herpes simplex virus type 1 appears to alter substrate specificity in the DNA polymerase.
We describe novel mutants of herpes simplex virus which are resistant to aphidicolin. Their mutant phenotypes suggest that they encode DNA polymerases with altered substrate recognition. This conclusion is based on their abnormal sensitivity to polymerase inhibitors and to the abnormal mutation rates exhibited by two of the mutants.
Hall
J D
JD
Department of Molecular and Cellular Biology, University of Arizona, Tucson 85721.
Woodward
S
S
D000998:Antiviral Agents / Q000494:pharmacology*; D016590:Aphidicolin; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000235:genetics* / Q000378:metabolism; D004224:Diterpenes / Q000494:pharmacology*; D004352:Drug Resistance, Microbial / Q000235:genetics; D009154:Mutation; D010641:Phenotype; D015183:Restriction Mapping; D018139:Simplexvirus / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D013379:Substrate Specificity; D010948:Viral Plaque Assay
D000998:Antiviral Agents; D004279:DNA, Viral; D004224:Diterpenes; D016590:Aphidicolin; D004259:DNA-Directed DNA Polymerase