Incidence of microscopically detectable degenerative characteristics in skeletal muscle of turkey.
1. The incidence of microscopically detectable degenerative characteristics in 5 skeletal muscles (m. pectoralis thoracicus, m. supracoracoideus, m. biceps femoris, m. semitendinosus, m. femorotibialis medius) of turkeys was investigated. 2. Samples were obtained from 30 Large White turkey males 14, 16 and 18 weeks old. Hyaline degeneration, infiltration of mononuclear cells and necrotic fibres were observed. 3. Individual fibres varied greatly in size and muscle fibre nuclei were often shrunken and pyknotic. 4. Weak and/or uniform reaction for Ca++-ATPase and SDH in all types of muscle fibres and loss of alkaline phosphatase activity in cell membranes were noted. A positive reaction for acid phosphatase occurred in regions of perivascular infiltration and in necrotic muscle fibres. The majority of muscle fibres possessed high activity for phosphorylase a and b. 5. Based on the use of fluorescein alpha-bungarotoxin conjugate, motor end-plates appeared to be morphologically intact. Direct immunofluorescence with anti-chicken IgG showed positive reaction in muscle fibres undergoing necrosis and in the involved connective tissue. 6. Degenerative changes varied with age and were most marked in the oldest birds. 7. Because gross degenerative symptoms were absent from both the birds and the meat from them, the condition appears to be either different from or a precursor to the degenerative myopathy characterised by other authors.
Sosnicki
A
A
Muscle Biology Laboratory, University of Wisconsin, Madison 53706.
Cassens
R G
RG
McIntyre
D R
DR
Vimini
R J
RJ
Greaser
M L
ML
D000375:Aging; D000818:Animals; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D008297:Male; D009119:Muscle Contraction; D009132:Muscles / Q000201:enzymology / Q000473:pathology* / Q000503:physiopathology; D009135:Muscular Diseases / Q000473:pathology / Q000503:physiopathology / Q000662:veterinary*; D009336:Necrosis; D010762:Phosphorylase a / Q000378:metabolism; D010763:Phosphorylase b / Q000378:metabolism; D011201:Poultry Diseases / Q000473:pathology* / Q000503:physiopathology; D013385:Succinate Dehydrogenase / Q000378:metabolism; D014422:Turkeys
D013385:Succinate Dehydrogenase; D010762:Phosphorylase a; D010763:Phosphorylase b; D000252:Calcium-Transporting ATPases