Glycolytic activity in embryos of Pisum sativum and of non-dormant or dormant seeds of Avena sativa L. expressed through activities of PFK and PPi-PFK.
A quantitative cytochemical assay for PPi-PFK activity in the presence of Fru-2,6-P2 is described along with its application to determine levels of activity in embryos of Pisum sativum and Avena sativa. The activity of ATP-PFK has also been studied in parallel as have PFK activities during the switch from dormant to non-dormant embryos in Avena sativa. PPi-PFK activity has been demonstrated in all tissues of Pisum sativum embryos and of Avena sativa embryos including the scutellum and the aleurone layers. The PPi-PFK activity was greater than that of ATP-PFK in both dormant and non-dormant seeds though with only marginally more activity in the dormant as opposed to the non-dormant state.
Corbineau
F
F
Laboratoire de Physiologie des Organes Végétaux après Récolte, C.N.R.S., Meudon, France.
Carmignac
D F
DF
Gahan
P B
PB
Maple
A J
AJ
D000818:Animals; D004625:Embryo, Nonmammalian / Q000201:enzymology*; D007887:Fabaceae / Q000201:enzymology*; D006651:Histocytochemistry / Q000379:methods; D010732:Phosphofructokinase-1 / Q000378:metabolism*; D010770:Phosphotransferases / Q000378:metabolism*; D010944:Plants / Q000196:embryology / Q000201:enzymology*; D010946:Plants, Medicinal; D012639:Seeds / Q000201:enzymology
D010770:Phosphotransferases; D010732:Phosphofructokinase-1; C022502:pyrophosphate-fructose 6-phosphate 1-phosphotransferase