Molecular cloning and localization to chromosome 6 of mouse INT1L1 gene.
The human INT1L1 gene, which exhibits homology to the protooncogene INT1 is very closely linked to the MET gene and cystic fibrosis locus on human chromosome 7. In the present study we have isolated overlapping genomic clones that correspond to the mouse homolog of the INT1L1 gene and have used the cloned DNA as probes to examine the distribution of the mouse INT1L1 gene within a series of 35 mouse-hamster somatic cell hybrids. These analyses have localized the INT1L1 gene to mouse chromosome 6. In addition, we demonstrate that the mouse INT1L1 and MET genes are coamplified in lines of spontaneously transformed mouse NIH3T3 cells, indicating that these genes may remain closely linked within the mouse genome.
Chan
A M
AM
Institute of Cancer Research, Chester Beatty Laboratories, London, UK.
Hilkens
J
J
Kroezen
V
V
Mitchell
P J
PJ
Scambler
P
P
Wainwright
B J
BJ
Williamson
R
R
Cooper
C S
CS
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002461:Cell Line, Transformed; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004247:DNA; D015342:DNA Probes; D005784:Gene Amplification; D015723:Gene Library; D008040:Genetic Linkage; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D008969:Molecular Sequence Data; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D011519:Proto-Oncogenes; D012689:Sequence Homology, Nucleic Acid; D051153:Wnt Proteins; D051154:Wnt2 Protein; D029961:Zebrafish Proteins
D015342:DNA Probes; D011518:Proto-Oncogene Proteins; C495244:WNT2 protein, human; D051153:Wnt Proteins; D051154:Wnt2 Protein; D029961:Zebrafish Proteins; D004247:DNA