Voltage-dependent calcium current in adherent mouse 3T3 fibroblasts.
Whole-cell recording was performed on adherent mouse Swiss 3T3 fibroblasts. Depolarizations from a holding potential of -100 mV gave rise to a transient inward current. The voltage dependence, kinetic properties, and ionic selectivity of this current are identical to those described in the same cells kept in suspension after detachment from the culture dish [A. Pandiella, A. Malgaroli, J. Meldolesi, and L.M. Vicentini (1987) Exp. Cell. Res. 170, 175-185; W.H. Moolenaar, L.G.J. Tertoolen, and S.W. de Laat (1984) J. Biol. Chem. 259, 8066-8069].
Peres
A
A
Department of General Physiology and Biochemistry, University of Milan, Italy.
Sturani
E
E
Zippel
R
R
D000200:Action Potentials; D000818:Animals; D002104:Cadmium / Q000502:physiology; D015220:Calcium Channels / Q000502:physiology*; D002448:Cell Adhesion; D002460:Cell Line; D004594:Electrophysiology; D005347:Fibroblasts / Q000502:physiology*; D008274:Magnesium / Q000502:physiology; D051379:Mice
D015220:Calcium Channels; D002104:Cadmium; D008274:Magnesium