Heparitinases facilitate separation of disaccharides of heparan sulfate isomers in human arteries using high-performance liquid chromatography.
The constituents of heparan sulfate isomers in human arteries were analysed at the disaccharide unit by high-performance liquid chromatography. Heparitinases I and II facilitated differentiation of six unsaturated disaccharides from heparan sulfate isomers. The variously sulfated disaccharide components of these heparan sulfate isomers were detected after digestion with heparitinases I and II. The heparan sulfate isomers in the aorta and pulmonary arteries were found to consist of various disaccharide units. These heparan sulfate isomers in the arteries are apparently formed during the process of aging and may influence arterial matrix components.
Murata
K
K
Medical Center, Tokyo National University of Arts and Music, Japan.
Yokoyama
Y
Y
Yoshida
K
K
D000293:Adolescent; D000328:Adult; D000368:Aged; D000375:Aging / Q000378:metabolism; D001011:Aorta / Q000032:analysis; D001158:Arteries / Q000032:analysis*; D002648:Child; D002851:Chromatography, High Pressure Liquid; D004187:Disaccharides / Q000032:analysis*; D006025:Glycosaminoglycans / Q000032:analysis*; D006497:Heparan Sulfate / Q000032:analysis*; D006801:Humans; D008875:Middle Aged; D011133:Polysaccharide-Lyases; D011651:Pulmonary Artery / Q000032:analysis
D004187:Disaccharides; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D011133:Polysaccharide-Lyases; C021349:heparitinsulfate lyase