Endothelin stimulates accumulations of cellular atrial natriuretic peptide and its messenger RNA in rat cardiocytes.
The effect of endothelin-1 (ET-1) on secretion and synthesis of rat atrial natriuretic peptide (rANP) as well as its mRNA levels was studied in primary cultures of neonatal rat atrial cardiocytes. ET-1 dose-dependently (10(-10)-10(-7) M) increased media and cellular rANP-like immunoreactivity as well as its cytoplasmic mRNA levels in rat cardiocytes during 24 hrs incubation. These results suggest that ET-1 directly stimulates expression of the rANP gene in cardiocytes, thereby leading to enhanced synthesis and secretion of rANP.
Fukuda
Y
Y
Department of Pediatrics, Kansai Medical University, Osaka, Japan.
Hirata
Y
Y
Taketani
S
S
Kojima
T
T
Oikawa
S
S
Nakazato
H
H
Kobayashi
Y
Y
D000818:Animals; D000831:Animals, Newborn; D009320:Atrial Natriuretic Factor / Q000096:biosynthesis* / Q000235:genetics; D015152:Blotting, Northern; D002478:Cells, Cultured; D016232:Endothelins; D004730:Endothelium, Vascular / Q000502:physiology; D006325:Heart Atria / Q000187:drug effects; D007700:Kinetics; D009206:Myocardium / Q000378:metabolism*; D010455:Peptides / Q000494:pharmacology*; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics*; D051381:Rats
D016232:Endothelins; D010455:Peptides; D012333:RNA, Messenger; D009320:Atrial Natriuretic Factor