title: Glucose uptake and flux through phosphofructokinase in wounded rat skeletal muscle.
abstract: Skeletal muscle injured with lambda-carrageenan has increased aerobic glycolysis. To assess the regulation of this process, the tissue concentrations of glycolytic intermediates, the flux through phosphofructokinase (PFK), and the intracellular concentrations of PFK effectors were examined in wounded rat skeletal muscle and in macrophages, the predominant inflammatory cell in the early stages of this wound model. Autoradiography demonstrated increased 2-deoxy-D-glucose uptake in wounded tissue compared with nonwounded muscle. 2-Deoxy-D-glucose was localized to the cellular infiltrate. The glycolytic intermediate concentrations demonstrated a facilitation of PFK in macrophages and wounded tissue as compared with nonwounded muscle. Wounded tissue had twice the flux through PFK compared with nonwounded muscle (10.0 +/- 0.6 wounded vs. 4.9 +/- 0.4 mumol.h-1.g-1 nonwounded). Macrophages had the highest flux through PFK (63.7 +/- 5.7 mumol.h-1.g-1) and when coincubated with muscle, the combined flux through PFK was equal to that of wounded muscle. The increase in glycolysis associated with wounded tissue may be explained by increased glucose uptake and increased flux through PFK by the inflammatory cells present in wounded tissue.
authors.affiliation: Department of Surgery, Brown University, Providence, Rhode Island 02902.
authors.forename: J
authors.initials: J
authors.lastname: Forster
authors.forename: A S
authors.initials: AS
authors.lastname: Morris
authors.forename: J D
authors.initials: JD
authors.lastname: Shearer
authors.forename: B
authors.initials: B
authors.lastname: Mastrofrancesco
authors.forename: K C
authors.initials: KC
authors.lastname: Inman
authors.forename: R G
authors.initials: RG
authors.lastname: Lawler
authors.forename: W
authors.initials: W
authors.lastname: Bowen
authors.forename: M D
authors.initials: MD
authors.lastname: Caldwell
mesh_terms: D000818:Animals; D001345:Autoradiography; D002351:Carrageenan / Q000633:toxicity; D003837:Deoxy Sugars / Q000378:metabolism*; D003847:Deoxyglucose / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008264:Macrophages / Q000378:metabolism; D008297:Male; D009132:Muscles / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D010732:Phosphofructokinase-1 / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D014316:Tritium
chemical_list: D003837:Deoxy Sugars; D014316:Tritium; D002351:Carrageenan; D003847:Deoxyglucose; D010732:Phosphofructokinase-1