Induction of chromosomal aberrations and SCE by camptothecin, an inhibitor of mammalian topoisomerase I.
The induction of chromosomal aberrations and sister-chromatid exchanges (SCE) was studied in human lymphocyte cultures treated with camptothecin (CM), an inhibitor of mammalian topoisomerase I. While no chromosome-type aberrations were found in G1-treated cells, instead there was a dose-dependent induction of chromatid-type aberrations. These types of chromosomal alteration were not induced during the treatment itself but during the S phase, as CM is not efficiently removed with the normal washing procedure after treatment.
Degrassi
F
F
Centro di Genetica Evoluzionistica, Università La Sapienza, Rome, Italy.
De Salvia
R
R
Tanzarella
C
C
Palitti
F
F
D002166:Camptothecin / Q000633:toxicity*; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D003470:Culture Media; D006801:Humans; D007399:Interphase / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects; D008940:Mitotic Index / Q000187:drug effects; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D059004:Topoisomerase I Inhibitors
D003470:Culture Media; D059004:Topoisomerase I Inhibitors; D002166:Camptothecin