title: Integrity of the exon 6 sequence is essential for tissue-specific alternative splicing of human leukocyte common antigen pre-mRNA.
abstract: By alternative splicing, exons 4, 5, and 6 of the human leukocyte common antigen (LCA) gene are included in B-cell mRNA but excluded from thymocyte mRNA. A mini-LCA gene that contains only LCA exons 2, 6, and 8 faithfully reproduces this tissue-specific alternative splicing in mouse B and thymocyte cell lines. Elimination of almost all of the intron sequences associated with exon 6 had no effect on the alternative splicing, while linker-scanning analysis showed that a significant length of the exon 6 sequence is essential for alternative splicing.
authors.affiliation: Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, Massachusetts.
authors.forename: A Y
authors.initials: AY
authors.lastname: Tsai
authors.forename: M
authors.initials: M
authors.lastname: Streuli
authors.forename: H
authors.initials: H
authors.lastname: Saito
mesh_terms: D000943:Antigens, Differentiation / Q000235:genetics*; D001402:B-Lymphocytes / Q000032:analysis; D001483:Base Sequence; D004252:DNA Mutational Analysis; D005091:Exons / Q000502:physiology*; D005822:Genetic Vectors; D006649:Histocompatibility Antigens / Q000235:genetics*; D006801:Humans; D007438:Introns / Q000502:physiology; D017493:Leukocyte Common Antigens; D008969:Molecular Sequence Data; D012326:RNA Splicing / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D013601:T-Lymphocytes / Q000032:analysis; D014162:Transfection
chemical_list: D000943:Antigens, Differentiation; D006649:Histocompatibility Antigens; D012333:RNA, Messenger; D017493:Leukocyte Common Antigens