Aminoglycoside-O-phosphotransferase (APH (3'')) activity in a clinical isolate of Acinetobacter calcoaceticus.
An isolate of Acinetobacter calcoaceticus var. anitratus containing an aminoglycoside-aminocyclitol antibiotic-modifying enzyme, which phosphorylates streptomycin at the 3" position, is described. The enzyme, APH(3"), was identified on the basis of its substrate specificity; in particular, its ability to phosphorylate streptomycin but not streptidine.
Elisha
B G
BG
Department of Medical Microbiology, University of Cape Town, Groote Schuur Hospital.
Steyn
L M
LM
D000150:Acinetobacter / Q000201:enzymology*; D010766:Phosphorylation; D010770:Phosphotransferases / Q000032:analysis*; D017853:Phosphotransferases (Alcohol Group Acceptor); D013307:Streptomycin / Q000378:metabolism*
D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C022679:streptomycin 3''-kinase; D013307:Streptomycin