Apparent absence of serotonin1B receptors in biopsied and post-mortem human brain.
In order to determine if post-mortem delays could account for the apparent absence of 5-HT1B sites reported in human brain, 5-HT1B binding parameters were determined in fresh and fresh-frozen cortical biopsies and compared to results obtained in post-mortem tissues. Binding parameters and in vitro receptor autoradiography were performed by using two different ligands which have been shown to label 5-HT1B sites, namely [3H]5-HT, in presence of 100 nM 8-OH-DPAT, and [125I]cyanopindolol, in presence of 10 microM (-)isoproterenol. No specific binding was detected with [125I] cyanopindolol in either fresh cortical biopsies or post-mortem tissues, suggesting that the apparent absence of 5-HT1B receptor sites reported earlier was not related to long post-mortem delays. Some specific labelling was seen with [3H]5-HT, in presence of 8-OH-DPAT. This binding, which is unlikely to be to the 5-HT1B type, could represent labelling to the 5-HT1D or 5-HT1E sites.
Martial
J
J
Douglas Hospital Research Centre, Quebec, Canada.
Lal
S
S
Dalpé
M
M
Olivier
A
A
de Montigny
C
C
Quirion
R
R
D017371:8-Hydroxy-2-(di-n-propylamino)tetralin; D001706:Biopsy; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D010869:Pindolol / Q000031:analogs & derivatives / Q000378:metabolism; D011180:Postmortem Changes; D011985:Receptors, Serotonin / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism; D013764:Tetrahydronaphthalenes / Q000378:metabolism; D014021:Tissue Preservation
D011985:Receptors, Serotonin; D013764:Tetrahydronaphthalenes; D012701:Serotonin; C047975:cyanopindolol; D017371:8-Hydroxy-2-(di-n-propylamino)tetralin; D010869:Pindolol