Enzyme histochemistry of monocytes/macrophages: a study in a murine model of immune complex-mediated glomerulonephritis.
In a model of immune complex glomerulonephritis in BALB/c mice, cells of monocyte/macrophage lineage (M phi), identifiable by electron microscopy, infiltrate the glomerulus. In spite of this, no unequivocal nonspecific esterase activity can be demonstrated histochemically in the glomeruli. On the other hand, many mononuclear cells with strong acid phosphatase activity are consistently present. This observation is in line with other studies that have demonstrated the heterogeneity of enzyme profiles in different M phi populations. Despite the wide acceptance of nonspecific esterases as markers for M phi, the present study indicates that exclusive reliance on a single marker in investigating the participation of M phi in a pathological process can lead to erroneous conclusions. Use of multiple markers and preferably multiple investigative modalities is recommended.
Iskandar
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Institute of Pathology, Case Western Reserve University, Cleveland, Ohio 44106.
Emancipator
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Pretlow
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D000818:Animals; D000936:Antigen-Antibody Complex; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D005921:Glomerulonephritis / Q000276:immunology / Q000473:pathology*; D006651:Histocytochemistry; D007105:Immune Complex Diseases / Q000276:immunology / Q000473:pathology*; D007678:Kidney Glomerulus / Q000473:pathology; D008264:Macrophages / Q000201:enzymology* / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D009000:Monocytes / Q000201:enzymology* / Q000473:pathology
D000936:Antigen-Antibody Complex; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase