[Human epidermal growth factor gene is expressed in saccharomyces cerevisiae].
Saccharomyces cerevisiae was transformed with plasmids containing the synthetic hEGF gene under control of a alpha-factor promoter. The expressed protein was efficiently secreted into the medium broth and was shown to be a biologically active hEGF.
Huang
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D001483:Base Sequence; D003001:Cloning, Molecular; D004815:Epidermal Growth Factor / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D005813:Genes, Synthetic; D000072235:Mating Factor; D010455:Peptides / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D010455:Peptides; D000072235:Mating Factor; D004815:Epidermal Growth Factor