Protein changes in bovine lymphoblastoid cells induced by infection with the intracellular parasite Theileria parva.
Protein and glycoprotein changes induced in bovine lymphoblasts by infection with Theileria parva were analyzed by high-resolution two-dimensional gel electrophoresis. Uninfected and infected cloned bovine T and B lymphoblasts were biosynthetically labeled with [35S]methionine and their two-dimensional autoradiographic patterns were compared with each other and with the pattern obtained using purified labeled schizonts. Ten proteins were found in infected cells which were not present in uninfected cells, and seven of these were detected in preparations of purified schizonts. Four glycoproteins were detected on the surface of infected cells labeled with [3H]borohydride while a major glycoprotein present on uninfected cells disappeared or was reduced in infected cells. Other minor changes in protein and glycoprotein patterns were also observed.
Sugimoto
C
C
International Laboratory for Research on Animal Diseases, Nairobi, Kenya.
Mutharia
L M
LM
Conrad
P A
PA
Dolan
T T
TT
Brown
W C
WC
Goddeeris
B M
BM
Pearson
T W
TW
D000818:Animals; D016782:Apicomplexa / Q000378:metabolism / Q000502:physiology*; D001402:B-Lymphocytes / Q000032:analysis / Q000469:parasitology*; D002417:Cattle; D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D008562:Membrane Glycoproteins / Q000032:analysis*; D008565:Membrane Proteins / Q000032:analysis*; D008715:Methionine / Q000032:analysis; D015800:Protozoan Proteins / Q000032:analysis*; D013601:T-Lymphocytes / Q000032:analysis / Q000469:parasitology*; D013801:Theileriasis / Q000469:parasitology
D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D015800:Protozoan Proteins; D008715:Methionine