title: Aldosterone binding sites along nephron of Xenopus and rabbit.
abstract: Distal segment of several amphibians exhibits aldosterone-modulated ion transport properties. On the other hand, A6 cells, derived from Xenopus laevis (XL) kidney, are aldosterone sensitive. We examined the distribution of aldosterone binding sites in isolated tubules of XL compared with rabbit. After incubation with 2 nM [3H]aldosterone, microdissected tubular segments from proximal (PT), distal straight segment (DST), and flask cell collecting (CT) tubules from XL and from rabbit cortical thick ascending limb (CTAL), connecting (CNT), and collecting (CCD) tubules were processed for dry film autoradiography. In XL, specific nuclear labeling of type I (mineralocorticoid) sites was restricted to DST. Labeling of type II (glucocorticoid) sites was present all along the tubule. No specific cytoplasmic labeling was observed, except for type II sites in PT. In the rabbit, aldosterone binds to both type I and type II sites in the three tubular segments studied. In these segments, the binding was about fourfold higher than in DST of XL. These results bring direct evidence in designating the distal tubule of amphibians as a target epithelium for aldosterone. In addition, they suggest that A6 cell line may derive from DST of the Xenopus nephron.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale (INSERM) U 246, Département de Biologie/SBCe, Gif-sur-Yvette, France.
authors.forename: A
authors.initials: A
authors.lastname: Gnionsahe
authors.forename: M
authors.initials: M
authors.lastname: Claire
authors.forename: N
authors.initials: N
authors.lastname: Koechlin
authors.forename: J P
authors.initials: JP
authors.lastname: Bonvalet
authors.forename: N
authors.initials: N
authors.lastname: Farman
mesh_terms: D000732:Androstanols / Q000378:metabolism; D000818:Animals; D001345:Autoradiography; D066298:In Vitro Techniques; D009399:Nephrons / Q000032:analysis*; D011817:Rabbits; D011965:Receptors, Glucocorticoid / Q000032:analysis*; D018161:Receptors, Mineralocorticoid; D014982:Xenopus laevis / Q000378:metabolism*
chemical_list: D000732:Androstanols; D011965:Receptors, Glucocorticoid; D018161:Receptors, Mineralocorticoid; C049154:11,17-dihydroxy-6-methyl-17-(1-propynyl)androsta-1,4,6-triene-3-one