title: Free radical-induced damage of cardiac sarcolemma (SL) and activity loss of beta-receptor adenylate cyclase system (beta-RAS). A comparison of the time courses.
abstract: In vitro studies have demonstrated that free radicals generated by Fe2+/Vit. C alter the beta-RAS function. SH-oxidation, peroxidation of sarcolemmal lipids and reaction of aldehydes (formed by lipid peroxidation) with the beta-RAS may contribute to this effect. In order to clarify the role of these reactions in respect to their ability to alter the beta-RAS function the time course of SH-oxidation, lipid peroxidation and formation of aldehyde reaction products has been studied. As result it is shown that SH-oxidation is nearly completed within 5 minutes and lipid peroxidation within 30 minutes after exposure to Fe2+/Vit. C. During this time period there was only a very small amount of aldehyde reaction products detectable. Therefore SH-oxidation and/or lipid peroxidation should be responsible for the activity loss of the beta-RAS.
authors.affiliation: Institute of Pathological and Clinical Biochemistry, Humboldt-University, Berlin, GDR.
authors.forename: I
authors.initials: I
authors.lastname: Schimke
authors.forename: A
authors.initials: A
authors.lastname: Haberland
authors.forename: L
authors.initials: L
authors.lastname: Will-Shahab
authors.forename: I
authors.initials: I
authors.lastname: Kuttner
authors.forename: B
authors.initials: B
authors.lastname: Papies
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D005296:Ferrous Compounds / Q000494:pharmacology*; D005609:Free Radicals; D006321:Heart / Q000187:drug effects*; D007545:Isoproterenol / Q000494:pharmacology; D007700:Kinetics; D015227:Lipid Peroxidation / Q000187:drug effects; D009206:Myocardium / Q000378:metabolism / Q000648:ultrastructure*; D011943:Receptors, Adrenergic, beta / Q000187:drug effects / Q000378:metabolism*; D012508:Sarcolemma / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D013552:Swine
chemical_list: D005296:Ferrous Compounds; D005609:Free Radicals; D011943:Receptors, Adrenergic, beta; C051140:iron(II)-ascorbic acid complex; D000262:Adenylyl Cyclases; D007545:Isoproterenol; D001205:Ascorbic Acid