Telomeric sequences derived from laser-microdissected polytene chromosomes.
Telomeric fragments from salivary gland squashes of Drosophila melanogaster Oregon R. were produced by a new microdissection technique, UV laser microbeam dissection. Microdissection, an essential step in microcloning procedures, is usually performed using micromanipulators and microneedles. Recently it has been shown that microdissection can be improved to very high precision if a laser coupled into a microscope is used. A laser microbeam, generated by an excimer pumped dye laser, allows chromosomes to be cut into slices of less than 0.5 micron. Here it is shown, that single copy DNA probes prepared from Drosophila chromosomes by laser microdissection and microcloning relocalize to the chromosomal regions from which they are derived. The combination of laser technique and microcloning provides an advantageous approach for rapid genetic analysis with potential for the study of genetic diseases and genome mapping.
Ponelies
N
N
Physikalisch-chemisches Institut der Universität, Heidelberg, Federal Republic of Germany.
Bautz
E K
EK
Monajembashi
S
S
Wolfrum
J
J
Greulich
K O
KO
D000818:Animals; D015139:Blotting, Southern; D002875:Chromosomes / Q000648:ultrastructure*; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D004331:Drosophila melanogaster / Q000235:genetics*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007834:Lasers; D008855:Microscopy, Electron, Scanning; D012091:Repetitive Sequences, Nucleic Acid
D004247:DNA