Islet transplantation to the renal subcapsular space in streptozotocin-diabetic rats: short-term effects on glucose-stimulated insulin secretion.
The immediate and short-term effects of transplantation of freshly isolated islets on glucose-stimulated insulin secretion were studied in streptozotocin-diabetic rats. At 2 days after the intravenous injection of streptozotocin (70 mg/kg), either 400 or 1000 freshly isolated, hand-picked islets were transplanted to the left renal subcapsular space. Intravenous glucose infusion (10 mg/min) performed 1, 3, 6, and 10 days after transplantation revealed that already at day 3, rats transplanted with 1000 islets had a significant plasma insulin response to glucose, though the response was lower than in healthy controls. At 6 and 10 days after transplantation, the 1000 islet-transplanted group of rats had a near-normal plasma insulin response to glucose. Basal plasma glucose levels were, however, still slightly elevated. Rats transplanted with 400 islets had a significant plasma insulin response at 10 days after transplantation, though the response was lower than in those transplanted with 1000 islets. It is concluded that a near-normal in vivo insulin secretory response to glucose is already obtained 6 days after transplantation with 1000 freshly isolated islets to the left renal subcapsular space in streptozotocin-diabetic rats. However, a slight hyperglycemia persists.
Rajab
A A
AA
Department of Surgery, Lund University, Sweden.
Ahrén
B
B
Bengmark
S
S
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D003921:Diabetes Mellitus, Experimental / Q000601:surgery*; D005947:Glucose / Q000494:pharmacology; D007328:Insulin / Q000097:blood / Q000378:metabolism*; D000078790:Insulin Secretion; D016381:Islets of Langerhans Transplantation; D008297:Male; D051381:Rats
D001786:Blood Glucose; D007328:Insulin; D005947:Glucose