Chemical modification potentiates paramylon induction of antimicrobial activity.
Induction of antimicrobial activity by two water-insoluble polysaccharides, paramylon and TAK, was significantly potentiated by introduction of positively charged groups (such as N,N-dimethylaminoethyl; N,N-diethylaminoethyl; and 2-hydroxy-3-trimethylammoniopropyl), but not by introduction of negatively charged groups (such as carboxymethyl or sulfate). Cross-linking of these derivatives with epichlorohydrin did not increase their potentiating activity. The effects of these derivatives did not always correlate with their ability to accumulate polymorphonuclear cells and to stimulate the generation of luminol-dependent chemiluminescence by peritoneal exudate cells.
Sakagami
H
H
First Department of Biochemistry, School of Medicine, Showa University, Tokyo, Japan.
Unten
S
S
Hanaoka
A
A
Ohsawa
N
N
Fujimaki
M
M
Komatsu
N
N
Ichikawa
S
S
Hata
N
N
Kondo
H
H
Konno
K
K
D000818:Animals; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects; D005056:Euglena gracilis / Q000254:growth & development; D005260:Female; D005936:Glucans / Q000138:chemical synthesis / Q000302:isolation & purification / Q000494:pharmacology*; D008163:Luminescent Measurements; D008407:Mast Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D008813:Mice, Inbred ICR; D008826:Microbial Sensitivity Tests; D013329:Structure-Activity Relationship
D000900:Anti-Bacterial Agents; D005936:Glucans; C003045:paramylon