title: The effects of local anesthetics on maternal and neonatal platelet function.
abstract: The effects of bupivacaine (B), lidocaine (L) and 2-chloroprocaine (C) on maternal (M) and neonatal (N) platelet function were studied using in vitro beta-thromboglobulin (beta-tg) release (radioimmunoassay), and in vitro platelet aggregation. Aggregation produced by adenosine diphosphate (ADP), epinephrine and collagen was measured in the presence of 1, 10, 100, 500 or 1000 micrograms/ml concentrations of B, L or C. In addition, spontaneous in vivo beta-tg release was measured in M and N blood. In vivo beta-tg level in M and N blood was approximately double that in non-pregnant subjects (p less than 0.025). In vitro beta-tg release in M and N samples was inhibited only at concentrations exceeding 1000 micrograms/ml, and the inhibition was less in M and N samples than in non-pregnant subjects. None of the anesthetics inhibited aggregation of M or N platelets at 1 and 10 micrograms/ml. Only concentrations of 500 micrograms/ml or greater consistently inhibited platelet aggregation produced by the three aggregants in M and N samples, and L was the least effective of the three agents. Neonatal platelet aggregation was affected more by local anesthetics than was maternal aggregation. It is concluded that plasma local anesthetic concentrations achieved during normal maternal epidural anesthesia do not affect M or N platelet aggregation or beta-tg release.
authors.affiliation: Department of Anesthesiology, New York University School of Medicine, New York.
authors.forename: G J
authors.initials: GJ
authors.lastname: Grant
authors.forename: S
authors.initials: S
authors.lastname: Ramanathan
authors.forename: N
authors.initials: N
authors.lastname: Patel
authors.forename: H
authors.initials: H
authors.lastname: Turndorf
mesh_terms: D000767:Anesthesia, Epidural; D000773:Anesthesia, Obstetrical; D000779:Anesthetics, Local / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D002045:Bupivacaine / Q000494:pharmacology; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D008012:Lidocaine / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D011247:Pregnancy; D011343:Procaine / Q000031:analogs & derivatives / Q000494:pharmacology; D001620:beta-Thromboglobulin / Q000378:metabolism
chemical_list: D000779:Anesthetics, Local; D010975:Platelet Aggregation Inhibitors; D001620:beta-Thromboglobulin; D011343:Procaine; C004616:chloroprocaine; D008012:Lidocaine; D002045:Bupivacaine