Glucagon-induced changes in fructose 2,6-bisphosphate and 6-phosphofructo-2-kinase in cultured rat foetal hepatocytes.
The sensitivity of 6-phosphofructo-2-kinase to glucagon and cyclic AMP was studied during the perinatal period. In liver homogenates from foetal and neonatal rats, incubation with cyclic AMP produced inactivation of 6-phosphofructo-2-kinase 3 h after birth. The maximal effect was obtained 12 h after birth. In primary cultures of hepatocytes from 22-day-old foetuses, glucogon induced an inhibition of 6-phosphofructo-2-kinase that required 45 min to reach the half-maximal effect. Cycloheximide prevented the glucagon-induced changes in this activity from cultured foetal hepatocytes. These results suggest that the adult form of 6-phosphofructo-2-kinase is rapidly induced after birth, probably by the hormonal changes that occur in this period.
Martin-Sanz
P
P
Instituto de Bioquimica del CSIC, Facultad de Farmacia, Universidad Complutense, Madrid, Spain.
Cascales
M
M
Boscá
L
L
D000818:Animals; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D003513:Cycloheximide / Q000494:pharmacology; D005635:Fructosediphosphates / Q000378:metabolism*; D005934:Glucagon / Q000378:metabolism*; D006598:Hexosediphosphates / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D025481:Phosphofructokinase-2; D010770:Phosphotransferases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D005635:Fructosediphosphates; D006598:Hexosediphosphates; C027652:fructose 2,6-diphosphate; D005934:Glucagon; D003513:Cycloheximide; D000242:Cyclic AMP; D010770:Phosphotransferases; D025481:Phosphofructokinase-2