The relationship between nodulin gene expression and the Rhizobium nod genes in Vicia sativa root nodule development.
The role of the Rhizobium nod genes in the induction of nodulin gene expression was examined by analyzing nodules formed on vetch roots by bacterial strains containing only the nod region. Introduction of an 11-kb cloned nod region of the R. leguminosarum sym plasmid pRL1JI into sym plasmid-cured rhizobia conferred on the recipient strains the ability to induce nodules in which all nodulin genes were expressed. This proves that from the sym plasmid only the nod region is involved in the induction of nodulin gene expression. A transconjugant of Agrobacterium carrying the same nod region induces nodules in which only early nodulin gene expression is detected. Thus, the nod region is essential for the induction of early nodulin gene expression. In this case, nodule cytology may indicate that a defense response of the plant interferes with the induction of late nodulin gene expression. Indirect evidence is presented that indeed the Rhizobium nod genes are also in some way involved in the induction of the expression of late noduling genes. The combination between histological data and pattern of nodulin gene expression furthermore reveals a correlation between nodule structure and nodulin gene expression. This correlation may aid in speculations about the functions of nodulins.
Nap
J P
JP
Department of Molecular Biology, Agricultural University, Wageningen, The Netherlands.
van de Wiel
C
C
Spaink
H P
HP
Moerman
M
M
van den Heuvel
M
M
Djordjevic
M A
MA
van Lammeren
A A
AA
van Kammen
A
A
Bisseling
T
T
D015153:Blotting, Western; D002876:Chromosomes, Bacterial / Q000502:physiology; D007887:Fabaceae / Q000033:anatomy & histology / Q000254:growth & development / Q000382:microbiology*; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D008565:Membrane Proteins; D009586:Nitrogen Fixation / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D010946:Plants, Medicinal; D010957:Plasmids; D015183:Restriction Mapping; D012231:Rhizobium / Q000235:genetics*
D008565:Membrane Proteins; D010940:Plant Proteins; C045625:nodulin