title: Kinetics of exchangeable protons in Z DNA: a UV resonance Raman study.
abstract: We have studied the hydrogen-deuterium exchange kinetics of the exchangeable protons of the poly(dG-dC).poly(dG-dC) in the Z form of the polymer, using resonance Raman spectroscopy with 257 nm and 284 nm excitation wavelengths. In our experimental conditions (4.5 M NaCl, phosphate buffer pH7, 2 degrees C) the two amino protons and the imino proton of guanine are exchanged with the same exchange half-time of 13 min, whereas the two amino protons of cytosine are exchanged with the same exchange half-time of 51 min.
authors.affiliation: LPCB, CNRS, Institut Curie, Paris, France.
authors.forename: A
authors.initials: A
authors.lastname: Laigle
authors.forename: L
authors.initials: L
authors.lastname: Chinsky
authors.forename: P Y
authors.initials: PY
authors.lastname: Turpin
authors.forename: B
authors.initials: B
authors.lastname: Jollès
mesh_terms: D001482:Base Composition; D003596:Cytosine; D004247:DNA; D003903:Deuterium; D017666:Deuterium Oxide; D006147:Guanine; D006859:Hydrogen; D007700:Kinetics; D011089:Polydeoxyribonucleotides; D011522:Protons; D013059:Spectrum Analysis, Raman / Q000379:methods; D014867:Water
chemical_list: D011089:Polydeoxyribonucleotides; D011522:Protons; D014867:Water; C019530:poly(dC-dG); D006147:Guanine; D006859:Hydrogen; D003596:Cytosine; D004247:DNA; D003903:Deuterium; D017666:Deuterium Oxide