[The effect of ascorbic acid in preservation solutions on the antioxidative potential of blood plasma during liver transplantation in miniature swine].
The antioxidative potential (AP) of blood plasma was defined for its O2-.-trapping capacity. The superoxide radicals were generated in a photochemical system and detected by luminol chemiluminescence. During the preparation phase of experimental liver transplantation on mini-pig the AP of peripheral blood increased twice. There is a 20-fold increase of AP one hour after the blood recirculation in transplant. An antioxidative defence of organ with ascorbic acid during the cold preservation period removed these increase. The possible mechanisms of the observed phenomena and the information significance of the AP measurement in blood plasma are discussed.
Popov
I
I
Institut für medizinische Immunologie, Bereichs Medizin (Charité) der Humboldt-Universität zu Berlin.
Gäbel
W
W
Lohse
W
W
Lewin
G
G
Richter
E
E
von Baehr
R
R
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D003080:Cold Temperature; D016031:Liver Transplantation; D008163:Luminescent Measurements; D010084:Oxidation-Reduction / Q000187:drug effects; D010100:Oxygen / Q000097:blood*; D013481:Superoxides / Q000378:metabolism; D013552:Swine; D013556:Swine, Miniature; D014021:Tissue Preservation
D013481:Superoxides; D001205:Ascorbic Acid; D010100:Oxygen
Einfluss von Ascorbinsäure in der Konservierungslösung auf das antioxidative Potential des Blutplasmas während der Lebertransplantation bei Minischweinen.