[Steroid hormone responsive cells in serum-free culture--analyses of hormone-mediated gene expression and cell growth].
Steroid hormone-responsive cell lines were clones from mouse mammary cancer (Shionogi Carcinoma 115) and Leydig cell tumor. SC-3 and SC-4 cells from Shionogi Carcinoma were androgen-responsive and -unresponsive in a serum-free medium, respectively. SC-3 cells secreted FGF-like growth factor as well as 24 K glycoprotein in response to androgen stimuli. B-1 and B-1F cells from mouse Leydig cell tumor were growth-stimulated in a serum-free medium by estrogen, androgen or retinoic acid. Transfection of ERE-TK-CAT gene into B-1F cells revealed that both estrogen and retinoic acid activated the CAT activity. In addition, the presence of corresponding receptors for steroid hormones or retinoic acid was demonstrated by hormone binding assays and/or Northern blot analysis. Thus, these serum-free culture systems seem to be very useful for analysing hormone action mechanisms in vitro.
Sato
B
B
Third Department of Internal Medicine, Osaka University Hospital, Japan.
D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002999:Clone Cells; D016895:Culture Media, Serum-Free; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D005938:Glucocorticoids / Q000494:pharmacology*; D007984:Leydig Cell Tumor / Q000235:genetics / Q000473:pathology*; D008297:Male; D008325:Mammary Neoplasms, Experimental / Q000235:genetics / Q000473:pathology*; D051379:Mice; D011987:Receptors, Steroid / Q000378:metabolism; D013736:Testicular Neoplasms / Q000235:genetics / Q000473:pathology*; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D000728:Androgens; D016895:Culture Media, Serum-Free; D004967:Estrogens; D005938:Glucocorticoids; D011987:Receptors, Steroid; D014212:Tretinoin