title: Rapid diagnosis of cytomegalovirus by indirect immunofluorescence assay with monoclonal antibody F6b in a commercially available kit.
abstract: Anti-human cytomegalovirus (CMV) monoclonal antibody (MAb) F6b produced by clone 95/12 was used for the rapid diagnosis (16 to 24 h) of CMV isolates in 308 clinical specimens and compared with classic isolation. MAb F6b gave 100% correlation with isolation. When this MAb was used for direct diagnosis with 212 urine and bronchoalveolar specimens, detection of CMV varied from 50 to 75% compared with viral isolation.
authors.affiliation: Diagnostics Pasteur, Marnes la Coquette, France.
authors.forename: G
authors.initials: G
authors.lastname: Lucas
authors.forename: J M
authors.initials: JM
authors.lastname: Seigneurin
authors.forename: J
authors.initials: J
authors.lastname: Tamalet
authors.forename: S
authors.initials: S
authors.lastname: Michelson
authors.forename: M
authors.initials: M
authors.lastname: Baccard
authors.forename: J F
authors.initials: JF
authors.lastname: Delagneau
authors.forename: P
authors.initials: P
authors.lastname: Deletoille
mesh_terms: D000911:Antibodies, Monoclonal; D003587:Cytomegalovirus / Q000302:isolation & purification*; D005455:Fluorescent Antibody Technique; D006801:Humans; D011933:Reagent Kits, Diagnostic
chemical_list: D000911:Antibodies, Monoclonal; D011933:Reagent Kits, Diagnostic