The diuretic and natriuretic action of human atrial natriuretic peptide in humans: lack of effect of exogenous insulin.
The interaction of exogenous, synthetic human atrial natriuretic peptide [(hANP) (ANF 99-126)] and of exogenous insulin was investigated in six healthy men and in seven type I diabetic patients using the euglycemic clamp technique. A primed-continuous (1.0 mU/kg x min) infusion of biosynthetic human insulin was administered to acutely raise and maintain plasma insulin concentrations at approximately 75 to 100 microU/mL during four hours while plasma glucose concentrations were maintained constant at the fasting level by a variable infusion of glucose. In healthy men a decrease in natriuresis (P less than .01) was seen during a euglycemic clamp study without exogenous hANP. No changes in diuresis and natriuresis were seen during a control experiment without exogenous insulin and glucose. Both in healthy men and in the type I diabetics sequential IV bolus doses of hANP of 100, 200, and 400 micrograms induced an increase in urine flow (P less than .01) and in natriuresis (P less than .01). In healthy men these effects were comparable to those achieved by hANP in the absence of induced hyperinsulinemia. It is concluded that the antinatriuretic action of insulin is of no major relevance in counteracting the pharmacologic action of hANP in healthy men. The effects of pharmacologic doses of hANP on diuresis and natriuresis in patients with type I diabetes mellitus is comparable to that in healthy men.
Vierhapper
H
H
Division of Clinical Endocrinology and Diabetes Mellitus, I. Medizinische Universitätsklinik, Vienna, Austria.
Bratusch-Marrain
P
P
Ratheiser
K
K
Waldhäusl
W
W
D000328:Adult; D009320:Atrial Natriuretic Factor / Q000008:administration & dosage* / Q000494:pharmacology; D001786:Blood Glucose / Q000032:analysis; D001834:Body Water; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism*; D015309:Glucose Clamp Technique; D006801:Humans; D007328:Insulin / Q000008:administration & dosage* / Q000378:metabolism / Q000494:pharmacology; D007671:Kidney Concentrating Ability / Q000187:drug effects; D008297:Male; D008875:Middle Aged; D009318:Natriuresis / Q000187:drug effects*; D012964:Sodium / Q000378:metabolism; D013223:Statistics as Topic
D001786:Blood Glucose; D007328:Insulin; D009320:Atrial Natriuretic Factor; D012964:Sodium