A novel deletion within the upstream regulatory region of episomal human papillomavirus type 16.
The pattern of human papillomavirus type 16 (HPV-16) integration was studied in 23 invasive carcinomas of the cervix using subgenomic probes. Seventeen tumours contained integrated HPV-16 and in 13 of these there was evidence of disruption within the E1-E2 open reading frames (ORFs). In all cases the upstream regulatory region (URR)-E6-E7 ORFs was maintained intact. Two independently derived tumours were infected with episomal wild-type HPV-16 and an episomal variant of HPV-16 containing a 325 bp deletion within the URR (positions 7598 to 17) and a point mutation at position 20 (A to C). This is the first report of a variant HPV-16 which is likely to be both defective and transmissible. Loss of E2 expression and deletion of a large portion of the URR may be two of the mechanisms leading to altered HPV-16 early gene expression in cervical tumours.
Tidy
J
J
Ludwig Institute for Cancer Research, St Mary's Hospital Medical School, London, U.K.
Vousden
K H
KH
Mason
P
P
Farrell
P J
PJ
D000230:Adenocarcinoma / Q000382:microbiology*; D001345:Autoradiography; D015139:Blotting, Southern; D002294:Carcinoma, Squamous Cell / Q000382:microbiology*; D003001:Cloning, Molecular; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D005260:Female; D005784:Gene Amplification; D005786:Gene Expression Regulation; D006801:Humans; D009154:Mutation; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D014412:Tumor Virus Infections / Q000382:microbiology*; D002583:Uterine Cervical Neoplasms / Q000382:microbiology*
D015344:DNA Probes, HPV; D004279:DNA, Viral