The Engelbreth-Holm-Swarm mouse tumor produces undersulfated heparan sulfate and oversulfated galactosaminoglycans.
Glycosaminoglycans were prepared from the Engelbreth-Holm-Swarm mouse tumor. Enzymatic analysis demonstrated heparan sulfate (95.8%) and chondroitinase ABC-sensitive galactosaminoglycans (4.2%). HPLC analysis of the disaccharide units showed that heparan sulfate chains were undersulfated on average, comprising approximately 30% nonsulfated and 60% N-sulfated disaccharide units with small proportions of other monosulfated and disulfated disaccharide units. In contrast, galactosaminoglycan chains were oversulfated, containing an appreciable proportion (15%) of a 4,6-disulfated (so-called E-type) disaccharide unit in addition to 51% of a 4-sulfated, 22% of a 6-sulfated, and 11% of a nonsulfated disaccharide unit. The significance of the oversulfated disaccharide structure is discussed in relation to the possible regulation of functions of hybrid proteoglycans from which the galactosaminoglycan chains are derived.
Sugahara
K
K
Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
Okumura
Y
Y
Yamashina
I
I
D000818:Animals; D002236:Carbohydrate Conformation; D002808:Chondroitin Lyases; D002851:Chromatography, High Pressure Liquid; D006025:Glycosaminoglycans / Q000378:metabolism*; D006497:Heparan Sulfate / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D010447:Peptide Hydrolases; D011133:Polysaccharide-Lyases; D011134:Polysaccharides / Q000378:metabolism*; D012513:Sarcoma, Experimental / Q000378:metabolism*; D013429:Sulfatases
D002808:Chondroitin Lyases; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D010447:Peptide Hydrolases; D011133:Polysaccharide-Lyases; D011134:Polysaccharides; D013429:Sulfatases; C007815:galactosaminoglycan; C021349:heparitinsulfate lyase