Identification of high affinity receptors for vasoactive intestinal peptide on human lymphocytes of B cell lineage.
Specific, high affinity receptors for vasoactive intestinal peptide (VIP) have been identified on a human pre-B cell line, Nalm 6, and on a human plasma cell line, Dakiki. The single class of high affinity sites exhibited a KD of 12.6 +/- 2.9 nM for VIP in Nalm 6 cells and 9.1 +/- 2.7 nM in Dakiki plasma cells. The homologous peptides, peptide histidine methionine (PHM), growth hormone releasing factor (GHRF), and secretin were all less effective than VIP in competitively inhibiting binding of 125I-VIP to Nalm 6 and Dakiki plasma membranes. The putative receptor was characterized as a 47-kDa protein using covalent cross-linking techniques and VIP stimulated adenylate cyclase in pre-B cells. Human lymphocytes of B cell lineage thus appear to express functional VIP receptors homologous to the receptor identified in T lymphoblasts, brain, pituitary, and intestine.
O'Dorisio
M S
MS
Department of Pediatrics, Ohio State University, Columbus 43205.
Shannon
B T
BT
Fleshman
D J
DJ
Campolito
L B
LB
D000262:Adenylyl Cyclases / Q000378:metabolism; D001402:B-Lymphocytes / Q000201:enzymology / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D015448:Leukemia, B-Cell / Q000201:enzymology / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D009363:Neoplasm Proteins / Q000378:metabolism; D010950:Plasma Cells / Q000378:metabolism; D011964:Receptors, Gastrointestinal Hormone / Q000032:analysis* / Q000502:physiology; D018005:Receptors, Vasoactive Intestinal Peptide; D013234:Stem Cells / Q000201:enzymology / Q000378:metabolism; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism* / Q000502:physiology
D008565:Membrane Proteins; D009363:Neoplasm Proteins; D011964:Receptors, Gastrointestinal Hormone; D018005:Receptors, Vasoactive Intestinal Peptide; D014660:Vasoactive Intestinal Peptide; D000262:Adenylyl Cyclases