Presence of a constitutive paracrine beta-interferon in v-mos-bearing nonmalignant reverted cells.
A stable nonmalignant revertant cell line was derived from Moloney murine sarcoma virus-transformed BALB/c cells after long-term cultivation in the presence of murine type I interferon (IFN). These cells gradually established resistance to exogenous IFN and were also seen to contain IFN-dependent proteins. The presence of an endogenous IFN was confirmed by the results of Northern blot analysis and in situ hybridization with an IFN-beta probe, showing that only mRNA specific for IFN-beta- could be found in the uninduced reverted cells. The latter synthesized only a small amount of IFN-beta protein and exhibited few IFN-specific membrane receptors, which bound recombinant IFN-beta with a high affinity. After treatment with IFN antibody, the overexpression of H-2 major histocompatibility antigen genes was significantly down-regulated. These findings strongly suggest the existence in this reverted cell line of a constitutive IFN which, acting through an autocrine and/or paracrine mechanism, might play a role in maintaining the reverted state.
Gerfaux
J
J
Unité 43 INSERM, Hôpital St. Vincent de Paul, Paris, France.
Sergiescu
D
D
Fournier
J G
JG
Pochart
F
F
Joret
A M
AM
Chany
C
C
D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D004351:Drug Resistance; D006183:H-2 Antigens / Q000235:genetics; D007370:Interferon Type I / Q000032:analysis* / Q000235:genetics / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D008980:Moloney murine sarcoma virus / Q000187:drug effects; D009857:Oncogenes; D012333:RNA, Messenger / Q000032:analysis; D011971:Receptors, Immunologic / Q000032:analysis; D017471:Receptors, Interferon
D006183:H-2 Antigens; D007370:Interferon Type I; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D017471:Receptors, Interferon