Cloning of a DNA region of Actinoplanes teichomyceticus conferring teicoplanin resistance.
Teicoplanin is a glycopeptide antibiotic, produced by Actinoplanes teichomyceticus, active against Gram positive bacteria and recently introduced into clinical practice. It blocks cell wall biosynthesis by inhibiting peptidoglycan polymerization. The mechanism(s) of resistance of the producer strains of this class of antibiotics have not yet been characterized. We have constructed a genomic bank of A. teichomyceticus in Streptomyces lividans. A clone from this bank, PTR168, was able to confer resistance to teicoplanin on its sensitive host. The restriction map of plasmid pTR168 and the hybridization pattern to A. teichomyceticus DNA were determined; we have also studied the mechanism of this resistance which seems correlated with a reduced binding of the antibiotic to the cell wall.
Moroni
M C
MC
Department of Microbiology, Lepetit Research Center, MDRI, Grenzano, Italy.
Granozi
C
C
Lorenzetti
R
R
Parenti
F
F
SosioM
Denaro
D000192:Actinomycetales / Q000235:genetics*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial; D005798:Genes, Bacterial; D006020:Glycopeptides; D015183:Restriction Mapping; D017334:Teicoplanin
D004269:DNA, Bacterial; D006020:Glycopeptides; D017334:Teicoplanin