The effect of cell synchronization on the efficiency of stable gene transfer by electroporation.
We synchronized thymidine kinase deficient mouse Ltk-cells by two different methods, hydroxyurea double-block treatment or aphidicolin single-block treatment and transformed them with the cloned herpes simplex virus thymidine kinase gene at various time intervals by the electroporation technique. Marked enhancement of stable transformation efficiency was observed at the time corresponding to the peak of G2/M phase. These results suggest that the G2/M phase is the most efficient period for stable gene transfer by electroporation.
Yorifuji
T
T
Department of Pediatrics, Faculty of Medicine, Kyoto University, Japan.
Tsuruta
S
S
Mikawa
H
H
D000818:Animals; D016590:Aphidicolin; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D004224:Diterpenes / Q000494:pharmacology; D004560:Electricity; D006918:Hydroxyurea / Q000494:pharmacology; D007399:Interphase; D007700:Kinetics; D051379:Mice; D008938:Mitosis; D018139:Simplexvirus / Q000201:enzymology / Q000235:genetics; D013937:Thymidine Kinase / Q000235:genetics*; D014162:Transfection; D014170:Transformation, Genetic
D004224:Diterpenes; D016590:Aphidicolin; D013937:Thymidine Kinase; D006918:Hydroxyurea