title: Recombinant interferon-gamma is a potent inhibitor of osteoblastic cell functions.
abstract: Interleukin-1 and tumor necrosis factor-stimulated bone resorption is mediated by osteoclast-activating factor elaborated by osteoblasts. Recombinant interferon-gamma inhibits stimulation of bone resorption by these cytokines. We examined here the effects of recombinant mouse interferon-gamma (rmIFN-G) on DNA and collagen synthesis, and on alkaline phosphatase (ALP) activity, in the osteoblastic cell line (MC3T3-E1) under confluent culture conditions. Addition of rmIFN-G to the cells markedly inhibited their DNA synthesis and ALP activity in dose- and culture time-related manner. Also we found that rmIFN-G decreased markedly collagen synthesis at day 3 after addition of the agent. These data indicate that rmIFN-G is a potent inhibitor of osteoblastic cell functions.
authors.forename: K
authors.initials: K
authors.lastname: Hirose
authors.forename: S
authors.initials: S
authors.lastname: Hanazawa
authors.forename: S
authors.initials: S
authors.lastname: Amano
authors.forename: H
authors.initials: H
authors.lastname: Kikuchi
authors.forename: A
authors.initials: A
authors.lastname: Takeshita
authors.forename: Y
authors.initials: Y
authors.lastname: Murakami
authors.forename: H
authors.initials: H
authors.lastname: Hashimoto
authors.forename: T
authors.initials: T
authors.lastname: Yokoyama
authors.forename: R
authors.initials: R
authors.lastname: Suyama
authors.forename: S
authors.initials: S
authors.lastname: Kitano
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001862:Bone Resorption; D002454:Cell Differentiation / Q000187:drug effects*; D003094:Collagen / Q000096:biosynthesis; D007371:Interferon-gamma / Q000494:pharmacology*; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism*; D011994:Recombinant Proteins
chemical_list: D011994:Recombinant Proteins; D007371:Interferon-gamma; D003094:Collagen; D000469:Alkaline Phosphatase