Morphological and biochemical alterations in growing rats induced by etretinate.
Etretinate is an aromatic retinoid extensively used on Dermatology. Its toxic effects, however, reduce its application from a clinical point of view. In the present paper, we study etretinate intoxication of 48 growing Wistar rats. The intoxication was for 12 weeks using etretinate doses of 0.5 and 6 (mg/kg)/day. The concentrations of etretinate in plasma and liver were determined. Total seric cholesterol and triglyceride concentrations were analyzed. Structural and ultrastructural histological studies of liver samples were carried out. Continuous etretinate ingestions seem to produce an alteration in the detoxication of enzymatic complexes in the growing rats with both the concentrations, due to the increase in etretinate blood plasma observed during the study. There is a relationship between the etretinate dose and its blood plasma concentration and toxic effect, but there is not with etretinate concentration in the liver. The blood plasma concentration of cholesterol and triglycerides is not related to histological liver lesions. The histological study confirms hepatotoxicity with both doses. Nevertheless, the anatomopathological lesions observed do not seem to be related to the blood plasma and liver etretinate concentrations.
Amo Bernal
C
C
Department of Pediatrics, Faculty of Medicine, University of Córdoba, Spain.
Méndez Sánchez
A
A
Sinisterra Gago
J V
JV
Sierra Plana
M A
MA
Fuentes García
A
A
D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology; D002784:Cholesterol / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D005050:Etretinate / Q000032:analysis / Q000097:blood / Q000633:toxicity*; D007202:Indicators and Reagents; D008099:Liver / Q000737:chemistry / Q000473:pathology; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains; D014280:Triglycerides / Q000097:blood
D007202:Indicators and Reagents; D014280:Triglycerides; D005050:Etretinate; D002784:Cholesterol