title: Angiotensin II stimulates an endogenous response in Xenopus laevis ovarian follicles.
abstract: While responses to angiotensin II have previously been induced in Xenopus laevis oocytes after injection of messenger RNA extracted from mammalian tissue, no endogenous responses of ovarian tissue to this hormone have been reported. Here we describe such an endogenous dose-dependent response to angiotensin II, detected by conventional electrophysiological techniques, in follicular oocytes. The ED50 of the response was estimated to be 0.15 +/- 0.07 microM (S.E.M.). Maximal depolarization, obtained at 1 microM angiotensin II, was 18.3 +/- 1.4 mV (n = 18, three experiments using oocytes from two toads, mean resting membrane potential = -42 +/- 2 mV). The response was absent from collagenase-treated oocytes or follicular oocytes treated with octanol, suggesting that the receptors are predominantly in the follicular layer surrounding the oocytes.
authors.affiliation: Department of Obstetrics and Gynaecology, Wellington School of Medicine, New Zealand.
authors.forename: M P
authors.initials: MP
authors.lastname: Lacy
authors.forename: R P
authors.initials: RP
authors.lastname: McIntosh
authors.forename: J E
authors.initials: JE
authors.lastname: McIntosh
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D008564:Membrane Potentials / Q000187:drug effects*; D003012:Microbial Collagenase; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D010053:Ovary / Q000187:drug effects / Q000502:physiology*; D014982:Xenopus laevis
chemical_list: D000804:Angiotensin II; D003012:Microbial Collagenase