Immunohistological identification of both infectious bursal and Marek virus antigens in the bursa of Fabricius.
Indirect Immunoperoxidase (IIP) and Avidin Biotin-Peroxidase Complex (ABC) techniques were used for the detection of Infectious Bursal Virus (IBV) and Marek Disease Virus (MDV) antigens in alcohol and formalin-fixed, paraffin-embedded lymphoid tissues from broilers. Both techniques appeared potentially useful for the diagnosis of both viral antigens in alcohol-fixed tissues, and allowed the observation of dual infection in the bursa of Fabricius of the studied animals in a natural infection.
Fernández
A
A
Martín de las Mulas
J
J
Sierra
M A
MA
Carranza
J
J
Jover
A
A
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002060:Bursa of Fabricius / Q000382:microbiology*; D002645:Chickens; D008381:Herpesvirus 2, Gallid / Q000276:immunology*; D007243:Infectious bursal disease virus / Q000276:immunology*; D008380:Marek Disease / Q000150:complications; D011201:Poultry Diseases / Q000382:microbiology; D012087:Reoviridae / Q000276:immunology*; D012088:Reoviridae Infections / Q000150:complications / Q000662:veterinary
D000956:Antigens, Viral