Ligand interactions of the cation-independent mannose 6-phosphate receptor. The stoichiometry of mannose 6-phosphate binding.
The interaction of the bovine cation-independent mannose 6-phosphate receptor with a variety of phosphorylated ligands has been studied using equilibrium dialysis and immobilized receptor to measure ligand binding. The dissociation constants for mannose 6-phosphate, pentamannose phosphate, bovine testes beta-galactosidase, and a high mannose oligosaccharide with two phosphomonoesters were 7 X 10(-6) M, 6 X 10(-6) M, 2 X 10(-8) M, and 2 X 10(-9) M, and the mol of ligand bound/mol of receptor monomer were 2.17, 1.85, 0.9, and 1.0, respectively. We conclude that the cation-independent mannose 6-phosphate receptor has two mannose 6-phosphate-binding sites/polypeptide chain.
Tong
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PY
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110.
Gregory
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Kornfeld
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D000117:Acetylglucosamine / Q000031:analogs & derivatives / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D002412:Cations; D002417:Cattle; D002846:Chromatography, Affinity; D003956:Dialysis; D004951:Esterification; D006600:Hexosephosphates / Q000378:metabolism*; D008297:Male; D008351:Mannans / Q000378:metabolism; D008360:Mannosephosphates / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism; D017527:Receptor, IGF Type 2; D011956:Receptors, Cell Surface / Q000378:metabolism*; D013737:Testis / Q000201:enzymology; D001616:beta-Galactosidase / Q000378:metabolism
D002412:Cations; D006600:Hexosephosphates; D008351:Mannans; D008360:Mannosephosphates; D009844:Oligosaccharides; D017527:Receptor, IGF Type 2; D011956:Receptors, Cell Surface; C027693:mannose-6-phosphate; C026146:N-acetylglucosamine-1-phosphate; C059709:pentamannose phosphate; C048076:methyl 6-(2-acetamido-2-deoxy-alpha-glucopyranosyl phosphate)-alpha-mannopyranoside; D001616:beta-Galactosidase; D000117:Acetylglucosamine