title: A rapid method for isolation of human hemoglobin benzo[a]pyrene diol epoxide derived adducts using high performance liquid chromatography.
abstract: A method is described to isolate rapidly human hemoglobin-benzo[a]pyrene diol epoxide adducts. A combination of 300 A pore size C4 reversed phase HPLC to effect separation of adducted protein from native protein, and mu-bore C18 reversed phase HPLC to isolate and partially characterize proteolytic peptide adducts (by UV), was used.
authors.affiliation: Department of Chemistry, Massachusetts Institute of Technology, Cambridge 02139.
authors.forename: S
authors.initials: S
authors.lastname: Naylor
authors.forename: L S
authors.initials: LS
authors.lastname: Gan
authors.forename: B W
authors.initials: BW
authors.lastname: Day
authors.forename: P L
authors.initials: PL
authors.lastname: Skipper
authors.forename: S R
authors.initials: SR
authors.lastname: Tannenbaum
mesh_terms: D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; D002851:Chromatography, High Pressure Liquid; D004101:Dihydroxydihydrobenzopyrenes; D006454:Hemoglobins / Q000302:isolation & purification*; D006801:Humans; D015394:Molecular Structure
chemical_list: D004101:Dihydroxydihydrobenzopyrenes; D006454:Hemoglobins; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide