The TP1 isolate of feline sarcoma virus encodes a fgr-related oncogene lacking gamma actin sequences.
We have isolated a new feline sarcoma virus, TP1-FeSV. The virus encodes a myristilated 83 kD gag-onc fusion protein displaying tyrosine kinase activity. We have established nonproducer cell lines lacking the TP1-FeSV associated helper virus (FeLV) and TP1-FeSV transfected NIH cell lines. Southern Blot analysis of genomic DNA and Northern Blot analysis of RNA isolated from these cell lines revealed that the oncogene of the TP1-FeSV isolate is related to the fgr oncogene of the GR-FeSV, but shows no hybridization to the gamma actin homologous sequences of the GR-FeSV. We have isolated TP1-FeSV specific clones from a genomic library. Restriction enzyme and sequence analysis showed that the TP1-FeSV genome consists of the first 1651 nucleotides of the gag gene, followed directly by fgr sequences. The TP1-FeSV fgr sequence starts 43 nucleotides after the beginning of the GR-FeSV fgr sequence. In contrast to the GR-FeSV fgr which has lost 13 amino acids of the c-fgr carboxy terminus, the TP1-FeSV fgr contains the complete carboxy terminus of the cellular fgr gene. The TP1-FeSV fgr sequence is followed by a unique 328 nucleotide long sequence of unknown origin. The 3' recombination site occurs within the pol gene, 460 nucleotides from the start of the env leader sequence. Comparison of the subcellular localization of the transforming proteins of TP1-FeSV and GR-FeSV show no striking difference; both molecules are in part associated with subcellular membrane/cytoskeletal fractions and form complexes with the cellular pp90 and pp50.
Kappes
B
B
Institut für Medizinische Virologie, Justus-Liebig-Universität, Giessen, Federal Republic of Germany.
Ziemiecki
A
A
Müller
R G
RG
Theilen
G H
GH
Bauer
H
H
Barnekow
A
A
D000199:Actins / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002451:Cell Compartmentation; D005814:Genes, Viral; D008969:Molecular Sequence Data; D011485:Protein Binding; D011505:Protein-Tyrosine Kinases; D015183:Restriction Mapping; D012190:Retroviridae / Q000302:isolation & purification*; D012191:Retroviridae Proteins / Q000235:genetics* / Q000378:metabolism; D015682:Retroviridae Proteins, Oncogenic; D005253:Sarcoma Viruses, Feline / Q000235:genetics / Q000302:isolation & purification*; D013329:Structure-Activity Relationship
D000199:Actins; D012191:Retroviridae Proteins; D015682:Retroviridae Proteins, Oncogenic; C052601:v-fgr protein, Feline sarcoma virus; D011505:Protein-Tyrosine Kinases