Role of the carboxyl-terminal region of the PVP protein (F0I subunit) in the H+ conduction of F0F1 H+-ATP synthase of bovine heart mitochondria.
By means of protein sequencing, labelling with thiol reagents and reconstitution studies it is shown that the carboxyl-terminal region of the PVP protein (F0I subunit, nuclear-encoded protein of Mr 25,000) of mitochondrial F0 promotes transmembrane proton conduction by F0 and the sensitivity of this process to oligomycin.
Papa
S
S
Institute of Medical Biochemistry and Chemistry, University of Bari, Italy.
Guerrieri
F
F
Zanotti
F
F
Houstĕk
J
J
Capozza
G
G
Ronchi
S
S
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D015151:Immunoblotting; D008565:Membrane Proteins / Q000378:metabolism*; D008929:Mitochondria, Heart / Q000201:enzymology*; D009840:Oligomycins / Q000494:pharmacology; D011485:Protein Binding; D006180:Proton-Translocating ATPases / Q000378:metabolism; D014357:Trypsin
D008565:Membrane Proteins; D009840:Oligomycins; D014357:Trypsin; D006180:Proton-Translocating ATPases