Effects of periodic weight support on medial gastrocnemius fibers of suspended rat.
Based on the current view that muscle fiber types reflect, at least to some degree, the probability of excitation of motor units in most normal movements, it was hypothesized that brief moderate periods of weight support would have little effect on a muscle that consists predominantly of high-threshold motor units. To test this hypothesis, the effects of 7 days of hindlimb suspension (HS) and HS plus intermittent weight support activity on the size and metabolic properties of individual fibers in the medial gastrocnemius (MG) were studied. HS resulted in a 40% decrease in the mean cross-sectional area of fibers that stain either dark or light for myosin adenosinetriphosphatase (ATPase) at an alkaline preincubation and are located in the deep region (i.e., close to the bone) of the MG. Dark ATPase fibers located in the superficial region were 17% smaller than controls (P greater than 0.05). Although the mean succinate and alpha-glycerophosphate dehydrogenase activities (optical density/min) per muscle fiber were not significantly (P greater than 0.05) affected by HS, it appeared that selected fibers of the deep MG region of HS rats had elevated enzyme activities. HS plus walking on a treadmill for 10 min every 6 h at 5 m/min and at a 19 degrees incline (total of 40 min/day) resulted in mean fiber cross-sectional area and enzyme activities nearer to control than the HS values. All adaptations were much less obvious in the fibers in a superficial (i.e., away from the bone) MG region.(ABSTRACT TRUNCATED AT 250 WORDS)
Graham
S C
SC
Department of Kinesiology, Brain Research Institute, Los Angeles, California.
Roy
R R
RR
Hauschka
E O
EO
Edgerton
V R
VR
D000222:Adaptation, Physiological; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D008297:Male; D009119:Muscle Contraction; D009132:Muscles / Q000033:anatomy & histology / Q000502:physiology*; D009133:Muscular Atrophy / Q000517:prevention & control; D010805:Physical Conditioning, Animal; D051381:Rats; D011919:Rats, Inbred Strains; D013385:Succinate Dehydrogenase / Q000378:metabolism
D005993:Glycerolphosphate Dehydrogenase; D013385:Succinate Dehydrogenase; D000251:Adenosine Triphosphatases