title: Interaction of hemin with erythrocyte membranes: alterations in the physical state of the major sialoglycoprotein.
abstract: Hemin has been shown to disrupt erythrocyte membrane skeletal protein-protein interactions, initially those involving band 4.1 (Shaklai et. al. (1986) Biochem. Int. 13, 467-477). We have used electron spin resonance (ESR) spin labels specific for cell-surface carbohydrates, skeletal membrane proteins, or bilayer lipids to find: (1) simultaneous reaction of the protein-specific spin label, MAL-6, which binds to skeletal protein SH residues, and 10 microM hemin suggested that hemin decreased skeletal protein-protein interactions; (2) 10 microM hemin markedly decreased (greater than 60%, P less than 0.001) the rotational motion of spin-labeled erythrocyte membrane cell-surface sialic acid residues, 70% of which are located on the major transmembrane sialoglycoprotein, glycophorin A; and (3) 10 microM hemin caused a small, but significant (P less than 0.02), decrease in the motion of a lipid bilayer specific spin label (5-NS) in the erythrocyte membrane. Since glycophorin A is reportedly linked to the erythrocyte membrane skeletal protein network by band 4.1, it is conceivable that hemin-induced disruption of skeletal protein interactions, particularly those of band 4.1, could subsequently lead to the alterations in the motion of cell-surface sialic acid presented in this report.
authors.affiliation: Department of Chemistry, University of Kentucky, Lexington 40506-0055.
authors.forename: J W
authors.initials: JW
authors.lastname: Wyse
authors.forename: D A
authors.initials: DA
authors.lastname: Butterfield
mesh_terms: D003497:Cyclic N-Oxides / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000648:ultrastructure; D005697:Galactosides / Q000097:blood; D006418:Heme / Q000031:analogs & derivatives*; D006427:Hemin / Q000494:pharmacology*; D006801:Humans; D008051:Lipid Bilayers; D008560:Membrane Fluidity / Q000187:drug effects; D012795:Sialoglycoproteins / Q000097:blood*; D013049:Spectrin / Q000502:physiology; D013438:Sulfhydryl Compounds
chemical_list: D003497:Cyclic N-Oxides; D005697:Galactosides; D008051:Lipid Bilayers; D012795:Sialoglycoproteins; D013438:Sulfhydryl Compounds; D013049:Spectrin; C017480:4-maleimido-2,2,6,6-tetramethylpiperidinooxyl; D006418:Heme; D006427:Hemin