Cross-reactivity of anti-ssDNA antibodies with heparan sulfate in patients with type I diabetes mellitus.
Anti-single-stranded-DNA antibodies cross-reactive with heparan sulfate were detected in serums of patients with type I (insulin-dependent) diabetes mellitus. The results suggested that heparan sulfate, the major glycosaminoglycan constituent of the glomerular basement membrane, may serve as a target antigen in vivo for cross-reactive anti-DNA antibodies. These polyreactive antibodies, directed toward repeating negatively charged units, may neutralize the heparan sulfate-associated polyanionic sites in the glomerulus, leading to an abnormal permeability of anionic plasma proteins.
Triolo
G
G
Istituto di Clinica Medica I and Istituto di Clinica Pediatrica, University of Palermo, Italy.
Giardina
E
E
Scarantino
G
G
Seddio
G
G
Cardella
F
F
Meli
F
F
Bompiani
G
G
D002648:Child; D003429:Cross Reactions; D004277:DNA, Single-Stranded / Q000276:immunology*; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006025:Glycosaminoglycans / Q000276:immunology*; D006497:Heparan Sulfate / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis
D004277:DNA, Single-Stranded; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D007074:Immunoglobulin G