Isotope effects on binding of NAD+ to lactate dehydrogenase.
The isotope effect on binding [4-2H]NAD+ and [4-3H]NAD+ to lactate dehydrogenase has been shown to be 1.10 +/- 0.03 by whole molecule isotope ratio mass spectrometry and 1.085 +/- 0.01 by 3H/14C scintillation counting. These values demonstrate that specific interactions of the nicotinamide ring with the enzyme make the C-H bond at C-4 less stiff in the binary complex.
LaReau
R D
RD
Department of Chemistry, Brown University, Providence, Rhode Island 02912.
Wan
W
W
Anderson
V E
VE
D000818:Animals; D001665:Binding Sites; D003903:Deuterium; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D013058:Mass Spectrometry; D015394:Molecular Structure; D009243:NAD / Q000378:metabolism*; D009536:Niacinamide; D012588:Scintillation Counting; D014316:Tritium
D009243:NAD; D014316:Tritium; D009536:Niacinamide; D003903:Deuterium; D007770:L-Lactate Dehydrogenase