Metastatic ability and differentiative properties of a new cell line of human embryonal rhabdomyosarcoma (CCA).
A new cell line (CCA) was established from a human embryonal rhabdomyosarcoma. It showed an "early" myogenic differentiation pattern: vimentin expression was found in 100% of cells, desmin in about 40% and myosin of the embryonic isoform in about 5%. Class I HLA expression on CCA cells was undeterctable but was greatly increased by in vitro treatment with human recombinant interferon-gamma and only marginally increased by human recombinant tumour necrosis factor-alfa. CCA cell line was tumorigenic in nude mice after either subcutaneous or intramuscular injection; macroscopic spontaneous metastases were not detected. The ability to induce metastatic nodules in the lungs was found when CCA cells were injected intravenously in cyclophosphamide-pretreated nude mice and, at low frequency, in untreated nude mice.
De Giovanni
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C
Centro Interdipartimentale di Ricerche sul Cancro G. Prodl., Università di Bologna, Italy.
Nanni
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P
Nicoletti
G
G
Ceccarelli
C
C
Scotlandi
K
K
Landuzzi
L
L
Lollini
P L
PL
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D002648:Child; D046508:Culture Techniques / Q000379:methods; D003520:Cyclophosphamide / Q000627:therapeutic use; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D011994:Recombinant Proteins; D012208:Rhabdomyosarcoma / Q000188:drug therapy / Q000473:pathology* / Q000503:physiopathology; D013724:Teratoma / Q000188:drug therapy / Q000473:pathology* / Q000503:physiopathology; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects
D011994:Recombinant Proteins; D007371:Interferon-gamma; D003520:Cyclophosphamide