Efficacy of idiotypic vaccination in mice bearing B-cell lymphoma.
Mice bearing B-cell lymphoma of BCL1 type were vaccinated with idiotype protein (IgM - Id) of IgM lambda type derived from BCL1 malignant cells. Mice were injected with different doses of BCL1 cells (10(3) and 10(6) cells) at the beginning of experimentation. The vaccination was started on days 5, 10 and 15. Mice were injected with either IgM - Id, IgM - Id conjugated with KLH, IgM - P (IgM lambda obtained from plasmacytoma) or were not immunized. Immunization with IgM - Id conjugated with KLH was more effective in preventing the death of mice injected with 10(3) and 10(6) cells than IgM - Id or IgM - P alone. There was a higher titre of anti-idiotype antibodies in mice immunized with IgM - Id - KLH than in those with IgM - Id only.
Khan
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Laboratoire de Cytologie, C.H.U., Faculty of Medicine, Rennes, France.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis; D007130:Immunoglobulin Idiotypes / Q000008:administration & dosage*; D007075:Immunoglobulin M / Q000008:administration & dosage*; D007167:Immunotherapy; D016393:Lymphoma, B-Cell / Q000276:immunology* / Q000628:therapy; D051379:Mice; D011863:Radioimmunoassay
D000888:Antibodies, Anti-Idiotypic; D007130:Immunoglobulin Idiotypes; D007075:Immunoglobulin M