title: Human serum antibody responses to Bordetella pertussis infection and pertussis vaccination.
abstract: We used an immunoblotting technique to compare the serum antibody responses to pertussis toxin (PT), filamentous hemagglutinin (FHA), a 69-kilodalton (kDa) adenylate cyclase-associated protein (69 KD protein), and Bordetella pertussis outer membrane proteins (OMPs) following either B. pertussis infection or immunization with whole-cell pertussis vaccine. Infection and vaccination induced nearly equally intense antibody responses to PT and to FHA, but vaccination induced stronger antibody responses to the 69 KD protein and to many OMPs. The importance of serum antibody responses to the 69 KD protein and to B. pertussis OMPs other than PT and FHA in conferring immunity to pertussis after vaccination is unknown. Serum antibody responses to PT following either infection or vaccination were almost exclusively to the 28-kDa enzymatic subunit (S1) and only rarely and weakly to the lesser molecular weight binding subunits (S2-S5).
authors.affiliation: Department of Communicable Diseases, St. George's Hospital Medical School, London, England.
authors.forename: M G
authors.initials: MG
authors.lastname: Thomas
authors.forename: K
authors.initials: K
authors.lastname: Redhead
authors.forename: H P
authors.initials: HP
authors.lastname: Lambert
mesh_terms: D037361:Adenylate Cyclase Toxin; D000262:Adenylyl Cyclases / Q000276:immunology; D000328:Adult; D000917:Antibody Formation; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D001886:Bordetella pertussis / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D007223:Infant; D037342:Pertussis Toxin; D010567:Pertussis Vaccine / Q000276:immunology; D010566:Virulence Factors, Bordetella / Q000276:immunology; D014917:Whooping Cough / Q000276:immunology*
chemical_list: D037361:Adenylate Cyclase Toxin; D001425:Bacterial Outer Membrane Proteins; D007136:Immunoglobulins; D010567:Pertussis Vaccine; D010566:Virulence Factors, Bordetella; D037342:Pertussis Toxin; D000262:Adenylyl Cyclases