Primary structure of the major O-glycosidically linked carbohydrate unit of human von Willebrand factor.
A reduced tetrasaccharide chain was obtained from human von Willebrand factor (vWF) by mild alkaline borohydride treatment. The purification of this O-glycosidically-linked oligosaccharide was achieved by serial affinity chromatography on immobilized concanavalin A and Lens culinaris agglutinin and finally gel filtration. Its structure was determined by a combination of methylation studies and 500 MHz 1H-NMR spectroscopy to be: NeuAc(alpha 2-3)Gal(beta 1-3)[NeuAc(alpha 2-6)]GalNAc-ol.
Samor
B
B
Centre Régional de Transfusion Sanguine, laboratoire d'Hémostase, Lille, France.
Michalski
J C
JC
Mazurier
C
C
Goudemand
M
M
De Waard
P
P
Vliegenthart
J F
JF
Strecker
G
G
Montreuil
J
J
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D006023:Glycoproteins / Q000737:chemistry* / Q000302:isolation & purification; D006027:Glycosides; D006801:Humans; D037102:Lectins; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D014841:von Willebrand Factor / Q000737:chemistry* / Q000302:isolation & purification
D006023:Glycoproteins; D006027:Glycosides; D037102:Lectins; D009844:Oligosaccharides; D014841:von Willebrand Factor