Release of cyclooxygenase products from primary cultures of tracheal epithelia of dog and human.
Release of cyclooxygenase products from primary cultures of dog or human tracheal epithelium was measured by radioimmunoassay. In both species, bradykinin, platelet-activating factor (PAF), and A23187 (a calcium ionophore) caused increases in the rate of release of prostaglandin (PG) E2 and smaller increases in PGF2 alpha, 6-keto-PGF1 alpha, and thromboxane B2 output. Isoproterenol, vasoactive intestinal peptide (VIP), methacholine, and leukotrienes C4 and D4 had no effect on release of these cyclooxygenase products. Gas chromatography-mass spectrometry showed that the ratio of PGE2 to PGD2 released from dog cells by A23187 was 30:1. Short-circuit current across dog cells was stimulated by bradykinin, A23187, PAF, VIP, methacholine, and isoproterenol. Only the responses to bradykinin and A23187 were reduced by pretreatment with indomethacin.
Widdicombe
J H
JH
Cardiovascular Research Institute, University of California, San Francisco 94143.
Ueki
I F
IF
Emery
D
D
Margolskee
D
D
Yergey
J
J
Nadel
J A
JA
D000818:Animals; D001920:Bradykinin / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002478:Cells, Cultured; D015237:Dinoprost / Q000378:metabolism*; D015232:Dinoprostone / Q000378:metabolism*; D004285:Dogs; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology; D007700:Kinetics; D010972:Platelet Activating Factor / Q000494:pharmacology; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D013189:SRS-A / Q000494:pharmacology; D013045:Species Specificity; D013929:Thromboxane B2 / Q000378:metabolism*; D014132:Trachea / Q000378:metabolism / Q000502:physiology*
D010972:Platelet Activating Factor; D013189:SRS-A; D000001:Calcimycin; D013929:Thromboxane B2; D015237:Dinoprost; D011451:Prostaglandin-Endoperoxide Synthases; D015232:Dinoprostone; D007545:Isoproterenol; D001920:Bradykinin