C3bi-binding protein on Candida albicans: temperature-dependent expression and relationship to human complement receptor type 3.
We investigated in detail the previously described capacity of pseudohyphae of Candida albicans to bind C3-coated particles. We show that the expression of the C3bi receptor of C. albicans was dependent upon the growth temperature of the fungi. C. albicans grown at 30 degrees C bound strongly to EAC1423bi, whereas those cells grown at 38.5 degrees C were completely devoid of this capacity. The molecule responsible for the attachment of EAC1423bi was heat labile and trypsin sensitive. Several, but not all, monoclonal antibodies to the alpha-chain of human complement receptor type 3 (CR3) stained C. albicans, and this reactivity was expressed in parallel with the capacity of C. albicans to bind EAC1423bi, i.e., both were dependent on the growth temperature of the fungi and were trypsin sensitive. In contrast to CR3, the binding of EAC1423bi to C. albicans did not require the presence of divalent cations. Rabbit immunoglobulin G antibodies directed against C. albicans inhibited the binding of EAC1423bi to C. albicans but not to human CR3. These inhibiting IgG antibodies recognized antigens expressed on the surface of pseudohyphae but not those of yeast cells. OKM-1, a monoclonal antibody to human CR3 inhibited the attachment of EAC1423bi to CR3 and also to C. albicans. OKM-1 precipitated a 130-kilodalton band from solubilized 125I-labeled C. albicans. We conclude that the complement receptors on C. albicans and human CR3 were antigenically related but not identical and that they differed in their functional characteristics.
Eigentler
A
A
Institute for Hygiene, Innsbruck, Austria.
Schulz
T F
TF
Larcher
C
C
Breitwieser
E M
EM
Myones
B L
BL
Petzer
A L
AL
Dierich
M P
MP
D000818:Animals; D000911:Antibodies, Monoclonal; D000946:Antigens, Fungal / Q000032:analysis / Q000276:immunology; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D002176:Candida albicans / Q000276:immunology / Q000378:metabolism* / Q000502:physiology; D002352:Carrier Proteins / Q000032:analysis* / Q000276:immunology; D005656:Fungal Proteins / Q000032:analysis* / Q000276:immunology; D006358:Hot Temperature; D006801:Humans; D007074:Immunoglobulin G / Q000502:physiology; D011233:Precipitin Tests; D011817:Rabbits; D011951:Receptors, Complement / Q000032:analysis* / Q000276:immunology; D017463:Receptors, Complement 3b; D014357:Trypsin
D000911:Antibodies, Monoclonal; D000946:Antigens, Fungal; D002352:Carrier Proteins; D005656:Fungal Proteins; D007074:Immunoglobulin G; D011951:Receptors, Complement; D017463:Receptors, Complement 3b; D014357:Trypsin