title: Synthesis of 11C-labelled thymidine for tumor visualization using positron emission tomography.
abstract: A no-carrier added synthesis of [11C]thymidine from cyclotron produced [11C]methyl iodide is presented. The bis-silylated derivative of the lithium salt of 5-bromo-2'-deoxyuridine is treated with [11C]methyl iodide in THF at -50 degrees C giving 20-30 mCi [11C]thymidine in a 40-70% radiochemical yield. Purification is performed by reversed phase chromatography on a C18 column. The whole procedure takes 25 min and the specific activity is about 20-30 mCi/mumol at the end of the synthesis.
authors.affiliation: Laboratoria voor Medische Biochemie en voor Klinische Analyse, Rijksuniversiteit Gent, Belgium.
authors.forename: E
authors.initials: E
authors.lastname: Poupeye
authors.forename: R E
authors.initials: RE
authors.lastname: Counsell
authors.forename: A
authors.initials: A
authors.lastname: De Leenheer
authors.forename: G
authors.initials: G
authors.lastname: Slegers
authors.forename: P
authors.initials: P
authors.lastname: Goethals
mesh_terms: D002250:Carbon Radioisotopes; D007553:Isotope Labeling / Q000295:instrumentation; D009369:Neoplasms / Q000000981:diagnostic imaging; D013936:Thymidine / Q000138:chemical synthesis* / Q000302:isolation & purification; D014055:Tomography, Emission-Computed
chemical_list: D002250:Carbon Radioisotopes; D013936:Thymidine