title: Antibody valence and induced signal transduction: the role of antibody valence in anti-CD3-induced signal transduction in isolated normal T cells.
abstract: This report provides direct evidence that protein kinase C (PKC) is activated in isolated, rigorously accessory cell (AC)-depleted T cells when the T cell antigen recognition complex is stimulated by divalent anti-CD3 monoclonal antibody. Anti-CD3 monoclonal antibody-stimulated PKC activation alone does not, however, directly stimulate T cell proliferation in the absence of AC. A rise in cytosolic calcium is the second signal believed to be of paramount importance in T cell activation. While mitogenic concentrations of some divalent anti-CD3 antibodies do not cause a rise in cytosolic calcium, polyvalent anti-CD3 does evoke increased intracellular Ca2+ in rigorously AC-depleted resting human T cells.
authors.affiliation: Charles A. Dana Research Institute, Boston, Massachusetts.
authors.forename: L B
authors.initials: LB
authors.lastname: Melton
authors.forename: F
authors.initials: F
authors.lastname: Lakkis
authors.forename: M
authors.initials: M
authors.lastname: Moscovitch-Lopatin
authors.forename: B R
authors.initials: BR
authors.lastname: Smith
authors.forename: J M
authors.initials: JM
authors.lastname: Williams
authors.forename: P M
authors.initials: PM
authors.lastname: Rosoff
authors.forename: T B
authors.initials: TB
authors.lastname: Strom
mesh_terms: D000911:Antibodies, Monoclonal / Q000502:physiology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D017252:CD3 Complex; D002118:Calcium / Q000378:metabolism; D006801:Humans; D007399:Interphase / Q000187:drug effects; D008213:Lymphocyte Activation / Q000187:drug effects; D011493:Protein Kinase C / Q000378:metabolism; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000201:enzymology / Q000276:immunology / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D011948:Receptors, Antigen, T-Cell; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium