Structure of the major O-glycosidic oligosaccharide of monkey erythrocyte glycophorin.
Sialic acids and the major O-glycosidic oligosaccharide of glycophorin MK from monkey (Japanese monkey, Macaca fuscata) erythrocyte membranes were characterized. N-Glycolylneuraminic acid (Neu5Gc) was found as the major sialic acid, which was confirmed by gas-liquid chromatography-mass spectrometry as the trimethylsilyl methyl ester. Three O-glycosidic oligosaccharide units were obtained from a tryptic glycopeptide that contained all of the carbohydrate units in glycophorin MK by mild alkaline borohydride/borotritide treatment. Carbohydrate analyses of the oligosaccharides revealed that they were composed of Neu5Gc, galactose and N-acetylgalactosaminitol in the molar ratios of 1:1:1 (trisaccharide), 2:1:1 (tetrasaccharide) and 3:1:1 (pentasaccharide). The content of oligosaccharide units was estimated to be 1:12:5 for penta-, tetra- and trisaccharide, respectively, based on the yields, the molecular weight, and the number of oligosaccharide attachment sites in the amino-acid sequence. The tetrasaccharide was the major oligosaccharide and its structure was proposed to be Neu5Gc alpha 2-3Gal beta 1-3[Neu5Gc alpha 2-6]GalNAcol.
Murayama
J
J
School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
Manabe
H
H
Fukuda
K
K
Utsumi
H
H
Hamada
A
A
D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D008401:Gas Chromatography-Mass Spectrometry; D006020:Glycopeptides / Q000302:isolation & purification; D006021:Glycophorins / Q000737:chemistry* / Q000302:isolation & purification; D006027:Glycosides; D007202:Indicators and Reagents; D008251:Macaca; D008745:Methylation; D008969:Molecular Sequence Data; D009438:Neuraminic Acids / Q000032:analysis; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification
D006020:Glycopeptides; D006021:Glycophorins; D006027:Glycosides; D007202:Indicators and Reagents; D009438:Neuraminic Acids; D009844:Oligosaccharides; C032592:N-glycolylneuraminic acid