Inhibition of gap-junctional intercellular communication between epithelial cells transformed by the activated H-ras-1 oncogene.
In order to study the effects of an activated H-ras-1 oncogene on gap-junctional intercellular communication, we introduced the EJ/T24 H-ras-1 oncogene into cells of the epithelial Clone 9-3 cell line. Gap-junctional intercellular communication was significantly reduced in H-ras-1-transformed Clone 9-3 derivatives; this result shows that transformation by the activated H-ras-1 oncogene can inhibit gap-junctional intercellular communication. We postulate that the activated H-ras-1 oncogene product could mediate this effect through a change in the phosphorylation of the major gap-junction protein.
Vanhamme
L
L
Département de Biologie Moléculaire, Université Libre de Bruxelles, Rhode-St-Genèse, Belgium.
Rolin
S
S
Szpirer
C
C
D000818:Animals; D002450:Cell Communication; D002471:Cell Transformation, Neoplastic; D002999:Clone Cells; D017630:Connexins; D005786:Gene Expression Regulation; D011905:Genes, ras; D007365:Intercellular Junctions / Q000502:physiology*; D008565:Membrane Proteins / Q000378:metabolism; D010766:Phosphorylation; D014162:Transfection
D017630:Connexins; D008565:Membrane Proteins