Determination of phosphoglucomutase (PGM1), acid phosphatase (ACP), and esterase D (ESD) in human bloodstains by hybrid isoelectric focusing (HIEF).
PGM1, ESD, and ACP were determined in bloodstain extracts by isoelectric focusing (IEF) with carrier ampholytes (CA) and HIEF. HIEF yields superior results in PGM typing from bloodstain extracts, whereas for ESD and ACP typing isoelectric focusing with carrier ampholytes seems to be the method of choice.
Muñoz-Barús
I
I
Department of Legal Medicine, Faculty of Medicine, University of Santiago, La Coruña, Spain.
Lareu
M V
MV
López-Rodriguez
I
I
Rodriguez-Calvo
M S
MS
Carracedo
A
A
D000135:Acid Phosphatase / Q000235:genetics*; D001788:Blood Grouping and Crossmatching; D001801:Blood Stains; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000235:genetics*; D006801:Humans; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000235:genetics*; D010641:Phenotype; D010733:Phosphoglucomutase / Q000235:genetics*; D011110:Polymorphism, Genetic
D007527:Isoenzymes; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; C020118:ESD protein, human; D000135:Acid Phosphatase; D010733:Phosphoglucomutase