Prostanoid involvement in the relaxation of in vitro mesenteric artery by bradykinin and des-Arg9-bradykinin.
Bradykinin (BK), des-Arg9-BK, prostaglandin E2, prostacyclin and angiotensin II all produced concentration-related relaxation of rabbit in vitro de-endothelialized superior mesenteric arterial ring preparations precontracted with phenylephrine. Responses were reproducible at time 1 h and 4 h after setting up the preparations. The cyclo-oxygenase inhibitor indomethacin (2.8 x 10(-6) M) and the protein synthesis inhibitor cycloheximide (7.2 x 10(-5) M) introduced at t = 0 h inhibited relaxant responses to BK, des-Arg9-BK and angiotensin II, but not prostaglandin E2 or prostacyclin at t = 1 h and t = 4 h. Cycloheximide and indomethacin applied to the tissues at t = 3 h inhibited relaxant responses to BK, des-Arg9-BK and angiotensin II (but not prostaglandin E2 or prostacyclin) at t = 4 h. It is concluded that BK, des-Arg9-BK and angiotensin II (but not prostaglandin E2 or prostacyclin) induced relaxant responses of the in vitro rabbit mesenteric artery are dependent upon the generation of a relaxant prostanoid from the tissue and that cycloheximide produces its effect in this tissue by a mechanism similar to that seen with indomethacin.
Nwator
I A
IA
Department of Physiological Sciences, University of Manchester, U.K.
Parsons
A A
AA
Whalley
E T
ET
D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D001920:Bradykinin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D003513:Cycloheximide / Q000494:pharmacology; D015232:Dinoprostone / Q000494:pharmacology; D011464:Epoprostenol / Q000494:pharmacology; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D008638:Mesenteric Arteries / Q000187:drug effects; D009126:Muscle Relaxation / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D010656:Phenylephrine / Q000494:pharmacology; D011453:Prostaglandins / Q000502:physiology*; D011817:Rabbits
D011453:Prostaglandins; D000804:Angiotensin II; C024626:bradykinin, des-Arg(9)-; D010656:Phenylephrine; D003513:Cycloheximide; D011464:Epoprostenol; D015232:Dinoprostone; D001920:Bradykinin; D007213:Indomethacin