title: The effect of phenytoin on the corticoidogenesis in the mitochondria and the endoplasmic reticulum of bovine adrenal cortex.
abstract: In the mitochondria and endoplasmic reticulum of bovine adrenal cortex, the influence of phenytoin on corticoidogenesis was studied using 0.1 microCi 4-14C-cholesterol, 0.01 microCi 4-14C-17 alpha-hydroxyprogesterone, deoxycorticosterone and NADPH-generating system. The cholesterol side-chain cleaving activity in the mitochondria was facilitated by 0.1 microM phenytoin, but was inhibited by higher concentrations. Steroid 11 beta-hydroxylase activity was inhibited by phenytoin. The steroid 21-hydroxylase activity in the endoplasmic reticulum, on the contrary, was mildly stimulated by 1 and 10 microM phenytoin. Phenytoin formed a modified type I spectrum in the adrenocortical mitochondria-malate complex.
authors.forename: M
authors.initials: M
authors.lastname: Funakoshi
mesh_terms: D000302:Adrenal Cortex / Q000187:drug effects; D000305:Adrenal Cortex Hormones / Q000096:biosynthesis*; D000818:Animals; D002417:Cattle; D004721:Endoplasmic Reticulum / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D010672:Phenytoin / Q000494:pharmacology*
chemical_list: D000305:Adrenal Cortex Hormones; D010672:Phenytoin