The cdc2 kinase is a nuclear protein that is essential for mitosis in mammalian cells.
A homolog of the fission yeast cdc2-encoded protein kinase (p34) is a component of M phase promoting factor in Xenopus oocytes. The homologous kinase in human HeLa cells is maximally active during mitosis, suggesting a mitotic role in mammalian somatic cells. This has been directly investigated by microinjection of anti-p34 antibodies into serum-stimulated rat fibroblasts. DNA synthesis was unaffected but cell division was quantitatively blocked in injected cells. Injection of antibodies against p13suc1, a component of the p34 kinase complex, did not block mitosis but caused mitotic abnormalities resulting in cells containing multiple micronuclei in the subsequent interphase. p34 localized in the nucleus during interphase. During mitosis, a fraction tightly associated with centrosomes. p13 was more evenly distributed between the nucleus and cytoplasm. These observations demonstrate that cdc2 is a nuclear and centrosomal protein that is required for mitosis in mammalian cells.
Riabowol
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K
Cold Spring Harbor Laboratory, New York 11724.
Draetta
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Brizuela
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Vandre
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Beach
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D000818:Animals; D016203:CDC2 Protein Kinase; D018797:Cell Cycle Proteins; D002460:Cell Line; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D005347:Fibroblasts / Q000378:metabolism*; D005656:Fungal Proteins / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007399:Interphase; D051379:Mice; D008938:Mitosis / Q000187:drug effects*; D009687:Nuclear Proteins / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D010750:Phosphoproteins / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D010770:Phosphotransferases / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D051381:Rats; D029702:Schizosaccharomyces pombe Proteins; D013552:Swine
D018797:Cell Cycle Proteins; D005656:Fungal Proteins; D009687:Nuclear Proteins; D010750:Phosphoproteins; D029702:Schizosaccharomyces pombe Proteins; C051655:Suc1 protein, S pombe; D004247:DNA; D010770:Phosphotransferases; D016203:CDC2 Protein Kinase