title: Mechanisms for regulating expression of membrane isoforms of Fc gamma RIII (CD16).
abstract: Granulocyte and natural killer (NK) cell Fc receptors for immunoglobulin G (CD16) differ in only a few amino acids, yet have phosphatidylinositol glycan (PIG) or polypeptide membrane anchors, respectively. Mutagenesis shows that anchoring is regulated by a serine residue near the PIG anchor attachment site in the extracellular domain. The NK cell isoform was not expressed on the surface of COS cells unless cotransfected with a subunit that was expressed in NK cells and that was identical to the gamma subunit of the high affinity IgE Fc receptor (Fc epsilon RI). However, the CD16 sequence and not expression of the gamma subunit is dominant in regulating PIG reanchoring.
authors.affiliation: Department of Pathology, Harvard Medical School, Boston, MA 02115.
authors.forename: M L
authors.initials: ML
authors.lastname: Hibbs
authors.forename: P
authors.initials: P
authors.lastname: Selvaraj
authors.forename: O
authors.initials: O
authors.lastname: Carpén
authors.forename: T A
authors.initials: TA
authors.lastname: Springer
authors.forename: H
authors.initials: H
authors.lastname: Kuster
authors.forename: M H
authors.initials: MH
authors.lastname: Jouvin
authors.forename: J P
authors.initials: JP
authors.lastname: Kinet
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics; D000943:Antigens, Differentiation / Q000235:genetics*; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D005803:Genes, Immunoglobulin; D006098:Granulocytes / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G; D007694:Killer Cells, Natural / Q000276:immunology; D007739:L Cells / Q000276:immunology; D051379:Mice; D009154:Mutation; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D011961:Receptors, Fc / Q000235:genetics*; D017452:Receptors, IgG; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D015703:Antigens, CD; D000943:Antigens, Differentiation; D007074:Immunoglobulin G; D012333:RNA, Messenger; D011961:Receptors, Fc; D017452:Receptors, IgG