title: Mouse thymoma cell line expresses a gluconeogenic enzyme, fructose 1,6-bisphosphatase.
abstract: Fructose 1,6-bisphosphatase was observed in a thymic lymphoma cell line, WEH17.1 (11.5 +/- 0.8 munits/mg cytosol protein). Only a trace amount of the enzyme activity was observed in normal thymus tissue. The WEH17.1 enzyme had a pH optimum at around 7.5. The AMP-concentration giving 50% inhibition of the activity was about 73 microM. That of the crude mouse liver enzyme was 35 microM. The antibodies against the liver and intestinal enzymes cross-reacted with the WEH17.1 enzyme with a lower affinity than the liver enzyme. Immunoblot showed that the subunit molecular weight of the WEH17.1 enzyme was the same as that of the liver enzyme.
authors.affiliation: Department of Biochemistry, Akita University School of Medicine, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Mizunuma
authors.forename: Y
authors.initials: Y
authors.lastname: Tashima
mesh_terms: D000249:Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D006597:Fructose-Bisphosphatase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015151:Immunoblotting; D007422:Intestines / Q000201:enzymology; D007527:Isoenzymes / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D051379:Mice; D013945:Thymoma / Q000201:enzymology*; D013950:Thymus Gland / Q000201:enzymology; D013953:Thymus Neoplasms / Q000201:enzymology*; D014407:Tumor Cells, Cultured
chemical_list: D007527:Isoenzymes; D000249:Adenosine Monophosphate; D006597:Fructose-Bisphosphatase