Generation of O2- and tyrosine cation-mediated chemiluminescence during the fertilization of sea urchin eggs.
Sea urchin eggs emit light in the visible region during their fertilization. Judging from the chemiluminescence spectra, one of the excited species generated is considered to have originated from a tyrosine cation radical-mediated reaction. Chemiluminescence probes such as luminol or a cypridina luciferin analog (See text) are useful for detecting the ovoperoxidase +H2O2 reaction associated with membrane hardening and O2- generation, respectively, during fertilization of sea urchin eggs.
Takahashi
A
A
Research Development Corporation of Japan, Kojinkai Central Hospital, Miyagi, Japan.
Totsune-Nakano
H
H
Nakano
M
M
Mashiko
S
S
Suzuki
N
N
Ohma
C
C
Inaba
H
H
D000818:Animals; D001386:Azides / Q000494:pharmacology; D002374:Catalase / Q000494:pharmacology; D002412:Cations; D005306:Fertilization; D005609:Free Radicals; D006861:Hydrogen Peroxide / Q000378:metabolism; D049449:Luminescence; D008165:Luminol / Q000494:pharmacology; D010063:Ovum / Q000378:metabolism*; D009195:Peroxidase / Q000037:antagonists & inhibitors / Q000378:metabolism; D012617:Sea Urchins; D019810:Sodium Azide; D013053:Spectrophotometry; D013482:Superoxide Dismutase / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism*
D001386:Azides; D002412:Cations; D005609:Free Radicals; D013481:Superoxides; D014443:Tyrosine; D008165:Luminol; D019810:Sodium Azide; D006861:Hydrogen Peroxide; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase