title: A region of a cyanobacterial genome required for sulfate transport.
abstract: Using the cysA locus of Salmonella typhimurium as a heterologous probe, we have cloned a region of the Anacystis nidulans R2 (Synechococcus PCC 7942) genome involved in sulfate assimilation. The 8.3-kilobase-pair region encodes at least five transcripts that cannot be detected unless the cells are deprived of sulfur. One of the genes in this region has been sequenced, and the protein that it encodes is homologous to a polypeptide component of other permease systems of Escherichia coli and Salmonella. Insertional inactivation of the putative sulfate permease gene, designated cysA, as well as of other genes within this region, results in cysteine auxotrophy, reduced sulfate uptake, and altered expression of soluble and cytoplasmic-membrane polypeptides associated with sulfur starvation.
authors.affiliation: Division of Molecular Plant Biology, University of California, Berkeley 94720.
authors.forename: L S
authors.initials: LS
authors.lastname: Green
authors.forename: D E
authors.initials: DE
authors.lastname: Laudenbach
authors.forename: A R
authors.initials: AR
authors.lastname: Grossman
mesh_terms: D000595:Amino Acid Sequence; D027321:Anion Transport Proteins; D001483:Base Sequence; D001692:Biological Transport; D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D026901:Membrane Transport Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D012486:Salmonella typhimurium / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013431:Sulfates / Q000378:metabolism*; D014158:Transcription, Genetic; D014170:Transformation, Genetic
chemical_list: D027321:Anion Transport Proteins; D004251:DNA Transposable Elements; D026901:Membrane Transport Proteins; D013431:Sulfates; D004247:DNA; C022702:sulfate permease; D004262:DNA Restriction Enzymes