Conformational modification of muscle phosphofructokinase from Jaculus orientalis upon ligand binding.
Phosphofructokinase from Jaculus orientalis muscle is an allosteric enzyme regulated by substrates and nucleotide effectors. The conformational modifications upon ligand binding were probed by UV difference spectra and reactivities of thiol groups towards dithiobisnitrobenzoate and N-ethylmaleimide. The binding of Fru-6-P induced significant perturbations in the environment of the aromatic residues and buried the most reactive on the three accessible cysteines per protomer. The same effect on thiol reactivity was observed upon binding of the activator AMP. Various perturbations of both difference spectra and thiol reactivity were detected in the presence of either Mg-ATP, an allosteric inhibitor, or Mg-ITP which is not an effector.
Tijane
M
M
Laboratoire de Biochimie, Faculté des Sciences, Rabat, Morocco.
el Hachimi
Z
Z
Benjouad
A
A
Desmadril
M
M
Yon
J M
JM
D000249:Adenosine Monophosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000494:Allosteric Regulation; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D003545:Cysteine / Q000032:analysis; D004228:Dithionitrobenzoic Acid; D004789:Enzyme Activation / Q000187:drug effects; D005033:Ethylmaleimide; D005636:Fructosephosphates / Q000378:metabolism; D007293:Inosine Triphosphate / Q000494:pharmacology; D009132:Muscles / Q000201:enzymology*; D010732:Phosphofructokinase-1 / Q000378:metabolism*; D011487:Protein Conformation; D012377:Rodentia / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013438:Sulfhydryl Compounds / Q000032:analysis
D005636:Fructosephosphates; D013438:Sulfhydryl Compounds; D007293:Inosine Triphosphate; C036863:magnesium ITP; D000249:Adenosine Monophosphate; C027618:fructose-6-phosphate; D000255:Adenosine Triphosphate; D004228:Dithionitrobenzoic Acid; D010732:Phosphofructokinase-1; D003545:Cysteine; D005033:Ethylmaleimide