Human atrial natriuretic factor (ANF). Characterisation of a monoclonal antibody panel and its use in radioimmunoassay.
The production of nine monoclonal antibodies to human atrial natriuretic factor (ANF 1-28) is described. All possible combinations of two antibodies failed to reveal any which could simultaneously bind ANF. Studies with ANF analogues and the antibodies having the three highest affinity values (KD = 5, 25 and 21 pM) indicated that the antibodies are directed to the central portion of the antigen molecule. The highest affinity antibody was able to replace polyclonal antisera in the radioimmunoassay of ANF in extracts of plasma.
Prowse
C
C
Department of Surgery, Edinburgh University, U.K.
George
E
E
Micklem
L R
LR
Hornsey
V
V
Brown
J
J
James
K
K
D000911:Antibodies, Monoclonal / Q000276:immunology*; D009320:Atrial Natriuretic Factor / Q000031:analogs & derivatives / Q000032:analysis* / Q000276:immunology; D001667:Binding, Competitive; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D011863:Radioimmunoassay / Q000379:methods*
D000911:Antibodies, Monoclonal; C472955:NPPA protein, human; D009320:Atrial Natriuretic Factor