title: Identification of calcium conducting channels in isolated boar sperm plasma membranes.
abstract: Ion channel recordings were obtained from liposomes containing purified boar sperm plasma membrane proteins using a tip-dip method. Liposomes prepared in HEPES-TRIS and clamped by electrodes containing Ba-HEPES displayed channel activity that was partially inhibited by verapamil or nitrendipine and completely inhibited by La3+. Reversal of current at pipette negative voltages was observed only when Ba2+ ions were also present in the bath solution. These data indicate that channels capable of carrying calcium currents are prominent components of the plasma membrane of mammalian sperm.
authors.affiliation: Department of Physiology, School of Medicine, Southern Illinois University, Carbondale 62901-6512.
authors.forename: T
authors.initials: T
authors.lastname: Cox
authors.forename: R N
authors.initials: RN
authors.lastname: Peterson
mesh_terms: D000818:Animals; D015220:Calcium Channels / Q000187:drug effects / Q000502:physiology*; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D004553:Electric Conductivity; D008081:Liposomes; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D009568:Nitrendipine / Q000494:pharmacology; D013094:Spermatozoa / Q000502:physiology*; D013552:Swine; D014700:Verapamil / Q000494:pharmacology
chemical_list: D015220:Calcium Channels; D008081:Liposomes; D009568:Nitrendipine; D014700:Verapamil