Time resolved analysis of tubulin phosphorylation during platelet activation.
Tubulin phosphorylation was analyzed during the different phases of platelet activation. Platelets preloaded with [32P]-phosphate were stimulated with collagen. Tubulin was immunoprecipitated from serial samples obtained during the activation process. The immunoprecipitates were resolved by SDS-polyacrylamide gel electrophoresis and autoradiographs analyzed by laser densitometry. Agonist induced dephosphorylation of platelets occurred after the onset of shape change at the time of initiation of the secretory release. The dephosphorylation was selective affecting specific peptides.
Berry
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Division of Hematology/Oncology, Memorial Hospital of RI, Pawtucket.
Dawicki
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DD
Steiner
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D000255:Adenosine Triphosphate / Q000378:metabolism; D001345:Autoradiography; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology*; D003094:Collagen / Q000494:pharmacology; D003488:Cyanogen Bromide; D003720:Densitometry; D011756:Diphosphates / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007163:Immunosorbent Techniques; D007700:Kinetics; D010710:Phosphates / Q000097:blood; D010766:Phosphorylation; D010974:Platelet Aggregation / Q000187:drug effects; D014404:Tubulin / Q000097:blood*
D011756:Diphosphates; D010710:Phosphates; D014404:Tubulin; D000255:Adenosine Triphosphate; D003094:Collagen; C003319:sodium pyrophosphate; D003488:Cyanogen Bromide