Effects of fibronectin and its peptide fragment on preimplantation mouse embryo.
To investigate the involvement of fibronectin in the early development of mouse embryo, embryos at the two-cell stage were cultured in serum-free Ham F-10 medium with or without fibronectin-related substances. When antibody raised against fibronectin was added at concentrations of 0, 10(-3), 10(-2), and 2 X 10(-2) (vol:vol), the percentages of cells in the blastocyst stage at 72 hours were 88.5%, 67.2%, 48.1%, and 0%, respectively. No inhibitory effects were observed with nonspecific antibody. A low concentration of exogenous fibronectin (up to 0.2 mumol/L) slightly enhanced mouse embryo development after 48 hours. However, a higher concentration (greater than 0.4 mumol/L) of fibronectin inhibited mouse embryo development. A competitive blocker of cell membrane fibronectin receptors, oligopeptide (Gly-Arg-Gly-Asp-Ser), inhibited mouse embryo development in a dose-dependent manner. Meanwhile, control peptide (Gly-Arg-Gly-Glu-Ser-Pro), which does not contain the crucial cell binding sequence, did not show any inhibitory effects. These findings suggest the necessity of endogenous fibronectin-blastomere interaction in early mouse embryo development.
Tsuiki
A
A
Department of Obstetrics and Gynecology, University of Miami School of Medicine, FL.
Preyer
J
J
Hung
T T
TT
D000818:Animals; D000906:Antibodies / Q000276:immunology; D001755:Blastocyst / Q000187:drug effects* / Q000502:physiology; D005314:Embryonic and Fetal Development / Q000187:drug effects; D005353:Fibronectins / Q000276:immunology / Q000494:pharmacology*; D051379:Mice; D009842:Oligopeptides / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology*; D013997:Time Factors; D001615:beta-Endorphin / Q000276:immunology
D000906:Antibodies; D005353:Fibronectins; D009842:Oligopeptides; D010446:Peptide Fragments; D001615:beta-Endorphin; C049554:glycyl-arginyl-glycyl-aspartyl-serine