Cloned endothelial cells from fetal bovine bone.
Primary cell cultures from fetal bovine sternum were developed in Coon's modified Ham's F-12 medium containing 10% Nu-Serum, 1% Ultroser-G, and 200 mg of galactose per liter. Clones were obtained by colony isolation; one clone, BBE-1, was selected for characterization. BBE-1 cells exhibited typical endothelial morphology by light and electron microscopy and immunofluorescence for factor VIII-related antigen throughout their life span of 8 months. The cells showed mitogenic responses to endothelial cell growth factor, basic fibroblast growth factor, insulin-like growth factor types I and II, platelet-derived growth factor, ascorbic acid, and progesterone. Parathyroid hormone stimulated intracellular accumulation of cAMP in BBE-1 cells but not in endothelial cells from two other tissues. These clonal cells provide a useful system for studies on bone vasculature, including its interactions with other bone cells.
Streeten
E A
EA
Metabolic Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892.
Ornberg
R
R
Curcio
F
F
Sakaguchi
K
K
Marx
S
S
Aurbach
G D
GD
Brandi
M L
ML
D000818:Animals; D001842:Bone and Bones / Q000166:cytology*; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002999:Clone Cells; D000242:Cyclic AMP / Q000378:metabolism; D004261:DNA Replication / Q000187:drug effects; D004727:Endothelium / Q000166:cytology* / Q000187:drug effects; D005333:Fetus; D006133:Growth Substances / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology; D007700:Kinetics
D006133:Growth Substances; D006493:Heparin; D000242:Cyclic AMP