title: Purification and primary structure of pro-aldosterone secretion inhibitory factor from bovine adrenal chromaffin cells.
abstract: This report documents the purification and the complete primary structure of bovine aldosterone secretion inhibitory factor precursor (pro-ASIF). ASIF-(1-103) contains at position 69-103 of its carboxy-terminal end the formely identified 35-amino acid biologically active form, hence confirming the endogenous character of ASIF in the adrenal medulla. Compared to atrial natriuretic factor (ANF)-related peptide precursors, bovine ASIF displays 65% homology at the carboxy-terminal while the remaining amino-terminal part shows much more variability. Bovine pro-ASIF exhibits 73% homology with porcine pro-brain natriuretic peptide (BNP), a situation reminiscent of the relationship of pro-ANF in various species. When ANF- and BNP-related COOH-termini of bovine, porcine, human, rat, and chicken are compared, it appears that bovine ASIF and porcine BNP are closely related and belong to the same family which however appears to be much more heterogenous than the ANF-related family. These results strongly suggest that bovine ASIF is encoded by a precursor gene similar to the gene of BNP but different from the one encoding ANF.
authors.affiliation: Laboratory of Molecular Pharmacology, Institut de Recherches Cliniques de Montréal, Québec, Canada.
authors.forename: T T
authors.initials: TT
authors.lastname: Nguyen
authors.forename: C
authors.initials: C
authors.lastname: Lazure
authors.forename: K
authors.initials: K
authors.lastname: Babinski
authors.forename: M
authors.initials: M
authors.lastname: Chrétien
authors.forename: A
authors.initials: A
authors.lastname: De Léan
authors.forename: H
authors.initials: H
authors.lastname: Ong
mesh_terms: D000313:Adrenal Medulla / Q000032:analysis*; D000595:Amino Acid Sequence; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000235:genetics; D001483:Base Sequence; D002417:Cattle; D002645:Chickens; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D009479:Neuropeptides / Q000235:genetics* / Q000302:isolation & purification; D011498:Protein Precursors / Q000235:genetics* / Q000302:isolation & purification; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D013552:Swine
chemical_list: D009479:Neuropeptides; D011498:Protein Precursors; C062306:pro-aldosterone secretion inhibitory factor; D009320:Atrial Natriuretic Factor