Lipoproteins: carriers of dehydroepiandrosterone fatty acid esters in human serum.
The association between lipoproteins, cholesterol and cholesteryl esters is very well known to facilitate both the transport in plasma and the entry of these non-polar compounds into the cellular compartment. However, recent observations suggest that in addition to cholesterol, lipoproteins contain several other steroids in their lipoidal metabolite forms which may be transported in the very low, low and high density lipoproteins in human serum. Using the important androgen and oestrogen precursor, dehydroepiandrosterone (DHEA), the biosynthetic formation of lipoidal DHEA was demonstrated in human serum. Serum was also fractionated into its lipoprotein components during the course of its incubation with tritiated DHEA. A progressive movement of the label from the fraction containing the conventional steroid binding-proteins to the lipoproteins was observed with the fraction containing the low density lipoproteins demonstrating the greatest incorporation of the label. This displacement occurred simultaneous to an extensive esterification of the labelled DHEA in serum. After 6 h of incubation, approx. 90% of the radioactivity in all the lipoprotein fractions was in the lipoidal form. Very little labelled lipoidal DHEA was associated with the serum protein fraction throughout the duration of incubation. These data suggest that lipoproteins act as the carriers of lipoidal DHEA following its formation from the non-conjugate parent steroid in serum.
Roy
R
R
MRC Group in Molecular Endocrinology, Le Centre Hospitalier de l'Université Laval, Ste-Foy, Québec, Canada.
Bélanger
A
A
D002784:Cholesterol / Q000097:blood; D002788:Cholesterol Esters / Q000097:blood; D003687:Dehydroepiandrosterone / Q000031:analogs & derivatives* / Q000097:blood*; D005227:Fatty Acids / Q000097:blood*; D006801:Humans; D007700:Kinetics; D008074:Lipoproteins / Q000097:blood*; D014316:Tritium
D002788:Cholesterol Esters; D005227:Fatty Acids; D008074:Lipoproteins; D014316:Tritium; D003687:Dehydroepiandrosterone; D002784:Cholesterol