Evidence for glycosyl-phosphatidylinositol anchoring of Toxoplasma gondii major surface antigens.
The four major surface antigens of Toxoplasma gondii tachyzoites (P43, P35, P30, and P22) were made water soluble by phosphatidylinositol-specific phospholipase C (PI-PLC). These antigens were biosynthetically labeled with 3H-fatty acids, [3H]ethanolamine, and [3H]carbohydrates. Treatment of 3H-fatty-acid-labeled parasite lysates with PI-PLC removed the radioactive label from these antigens. A cross-reacting determinant was exposed on these antigens after PI-PLC treatment.
Tomavo
S
S
Unite' 42, Institut National de la Recheche Médicale, Villeneuve d'Ascq, France.
Schwarz
R T
RT
Dubremetz
J F
JF
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D004983:Ethanolamines / Q000378:metabolism; D005944:Glucosamine / Q000378:metabolism; D006017:Glycolipids / Q000032:analysis*; D017261:Glycosylphosphatidylinositols; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D008358:Mannose / Q000378:metabolism; D051379:Mice; D009227:Myristic Acids / Q000378:metabolism; D010169:Palmitic Acids / Q000378:metabolism; D010716:Phosphatidylinositols / Q000032:analysis*; D011233:Precipitin Tests; D014122:Toxoplasma / Q000276:immunology*; D010738:Type C Phospholipases
D000953:Antigens, Protozoan; D000954:Antigens, Surface; D004983:Ethanolamines; D006017:Glycolipids; D017261:Glycosylphosphatidylinositols; D007457:Iodine Radioisotopes; D009227:Myristic Acids; D010169:Palmitic Acids; D010716:Phosphatidylinositols; D010738:Type C Phospholipases; D005944:Glucosamine; D008358:Mannose