Production of platelet-derived growth factorlike protein(s) by a human carcinoma cell line.
Human adrenal carcinoma cells (SW-13) produce and secrete autocrine growth factors. We now report that these cells secrete a factor that is biologically related to platelet-derived growth factor (PDGF). Serum-free medium conditioned by SW-13 cells stimulated incorporation of tritiated thymidine into DNA by quiescent Balb/c 3T3 cells in the presence of platelet-poor plasma. The conditioned media also competed with 125I-labeled PDGF for binding to PDGF receptors on human fibroblasts. Hybridization studies identified mRNA transcripts for PDGF-2/sis chains in logarithmically growing SW-13 cells. These data suggest secretion of PDGF-like mitogens by SW-13 cells.
O'Donnell
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KA
University of Maryland Cancer Center, Baltimore 21201.
Condon
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ME
Hamburger
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AW
D000310:Adrenal Gland Neoplasms / Q000378:metabolism*; D002460:Cell Line; D003470:Culture Media; D006801:Humans; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D011956:Receptors, Cell Surface / Q000378:metabolism; D017479:Receptors, Platelet-Derived Growth Factor; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured / Q000378:metabolism*
D003470:Culture Media; D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D017479:Receptors, Platelet-Derived Growth Factor