Effect of two different glucose concentrations on insulin receptor mRNA levels in human hepatoma HepG2 cells.
Glucose is known to affect mRNA levels of several genes. In order to investigate possible effects of glucose on insulin receptor mRNA levels, we cultured human hepatoma cells (HepG2) in two different culture media: DMEM containing 100 mg/dl glucose and DMEM containing 450 mg/dl glucose. Insulin receptor mRNA levels and insulin binding activity were reduced in HepG2 cultured at lower glucose concentrations. These data suggest that glucose affects insulin receptor gene expression.
Briata
P
P
Department of Internal Medicine, University of Genova, Italy.
Gherzi
R
R
Adezati
L
L
Cordera
R
R
D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects*; D005947:Glucose / Q000008:administration & dosage / Q000494:pharmacology*; D006031:Glycosylation; D006801:Humans; D007163:Immunosorbent Techniques; D007328:Insulin / Q000378:metabolism; D008113:Liver Neoplasms / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism*; D011972:Receptor, Insulin / Q000235:genetics* / Q000378:metabolism; D014407:Tumor Cells, Cultured
D003470:Culture Media; D007328:Insulin; D012333:RNA, Messenger; D011972:Receptor, Insulin; D005947:Glucose