Monoclonal antibodies recognizing transforming growth factor-beta. Bioactivity neutralization and transforming growth factor beta 2 affinity purification.
Four mAb able to recognize transforming growth factor-beta 2 (TGF-beta)2 were obtained. One of these mAb, 1D11.16, was able to neutralize the biological activity of both TGF-beta 1 and beta 2 in vitro. This was demonstrated in an Il-1, PHA-dependent thymocyte mitogenic assay that is inhibitable by TGF-beta in a dose-dependent manner. All four mAb recognized the dimeric form of TGF-beta 2 in Western blots. The mAb were also found to immunoprecipitate [125I]-TGF-beta 2. mAb 3C7.14 coupled to Sepharose could efficiently immunoaffinity purify TGF-beta 2 from a complex mixture of proteins. Affinity constants were determined for the four mAb and they ranged from 3.4 x 10(8) to 1.6 x 10(7) L/mol.
Dasch
J R
JR
Department of Immunology, Collagen Corporation, Palo Alto, CA 94303.
Pace
D R
DR
Waegell
W
W
Inenaga
D
D
Ellingsworth
L
L
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis / Q000502:physiology; D000915:Antibody Affinity; D000937:Antigen-Antibody Reactions; D001667:Binding, Competitive; D015153:Blotting, Western; D002846:Chromatography, Affinity; D003432:Cross-Linking Reagents; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D011233:Precipitin Tests; D011956:Receptors, Cell Surface / Q000032:analysis; D018125:Receptors, Transforming Growth Factor beta; D015291:Transforming Growth Factors / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism
D000911:Antibodies, Monoclonal; D003432:Cross-Linking Reagents; D011956:Receptors, Cell Surface; D018125:Receptors, Transforming Growth Factor beta; D015291:Transforming Growth Factors