Selenoprotein P receptor from rat.
Radioreceptor assay technology was used to show the presence in the rat of a receptor that binds selenoprotein P, a selenocysteine-containing rat plasma protein. 75Se-labeled selenoprotein P bound to testis, kidney, and liver membranes. The binding was specific in that increasing amounts of partially-fractionated rat plasma specifically displaced the binding of 75Se-labeled selenoprotein P to testis membrane in a competitive manner. 75Se-labeled selenoprotein P binding was saturable in the presence of increasing amounts of testis membranes. The binding of 75Se-labeled selenoprotein P was optimal at about pH 4.2. Several proteins and blood fractions had little or no significant effect on binding of 75Se-labeled selenoprotein P to testis membranes. All plasma sources tested specifically displaced 75Se-labeled selenoprotein P from testis membrane, indicating that selenoprotein P-related proteins may be widespread in nature. The study indicated that selenoprotein P has a receptor and is involved in selenium transport.
Gomez
B
B
Department of Food Science and Technology, University of California, Davis 95616.
Tappel
A L
AL
D000818:Animals; D001667:Binding, Competitive; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D011506:Proteins / Q000378:metabolism*; D011869:Radioligand Assay; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012643:Selenium / Q000378:metabolism*; D051149:Selenoprotein P; D051140:Selenoproteins; D013737:Testis / Q000378:metabolism
D011506:Proteins; D011956:Receptors, Cell Surface; D051149:Selenoprotein P; D051140:Selenoproteins; D012643:Selenium