Identification of calcium conducting channels in isolated boar sperm plasma membranes.
Ion channel recordings were obtained from liposomes containing purified boar sperm plasma membrane proteins using a tip-dip method. Liposomes prepared in HEPES-TRIS and clamped by electrodes containing Ba-HEPES displayed channel activity that was partially inhibited by verapamil or nitrendipine and completely inhibited by La3+. Reversal of current at pipette negative voltages was observed only when Ba2+ ions were also present in the bath solution. These data indicate that channels capable of carrying calcium currents are prominent components of the plasma membrane of mammalian sperm.
Cox
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Department of Physiology, School of Medicine, Southern Illinois University, Carbondale 62901-6512.
Peterson
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D000818:Animals; D015220:Calcium Channels / Q000187:drug effects / Q000502:physiology*; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D004553:Electric Conductivity; D008081:Liposomes; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D009568:Nitrendipine / Q000494:pharmacology; D013094:Spermatozoa / Q000502:physiology*; D013552:Swine; D014700:Verapamil / Q000494:pharmacology
D015220:Calcium Channels; D008081:Liposomes; D009568:Nitrendipine; D014700:Verapamil