Caged-Ca2+: a new agent allowing liberation of free Ca2+ in biological systems by photolysis.
The alkaline earth cation complexes of DM-nitrophen [1-(2-nitro-4,5-dimethoxyphenyl)-N,N,N',N'-tetrakis-[(oxycarbonyl) methyl]- 1,2-ethanediamine)] release the bound cation in the millisecond time range upon irradiation by a short UV-light pulse. This technique allows to generate cation (eg. Ca2+) concentration jumps or pulses in solution or in cellular systems. The physico-chemical properties of DM-nitrophen and its Mg2+, Ca2+ and Ba2+ complexes are investigated by employing spectrophotometric and potentiometric techniques. In case of Ca2+ a stability constant of the complex up to nearly 10(11) M-1 is found. The magnitude of representative Mg2+ and Ca2+ concentration jumps which can be generated under realistic experimental conditions are calculated on the basis of the thermodynamic parameters reported here.
Grell
E
E
Lewitzki
E
E
Ruf
H
H
Bamberg
E
E
Ellis-Davies
G C
GC
Kaplan
J H
JH
de Weer
P
P
D000085:Acetates / Q000032:analysis; D001464:Barium / Q000032:analysis; D002118:Calcium / Q000032:analysis; D002614:Chelating Agents / Q000032:analysis; D004492:Edetic Acid; D005029:Ethylenediamines / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D010782:Photolysis
D000085:Acetates; D002614:Chelating Agents; D005029:Ethylenediamines; C057014:DM-nitrophen; D001464:Barium; D004492:Edetic Acid; D002118:Calcium