Secretory pancreatic stone protein messenger RNA. Nucleotide sequence and expression in chronic calcifying pancreatitis.
The pancreatic stone protein and its secretory form (PSP-S) are inhibitors of CaCO3 crystal growth, possibly involved in the stabilization of pancreatic juice. We have established the structure of PSP-S mRNA and monitored its expression in chronic calcifying pancreatitis (CCP). A cDNA encoding pre-PSP-S has been cloned from a human pancreatic cDNA library. Its nucleotide sequence revealed that it comprised all but the 5' end of PSP-S mRNA, which was obtained by sequencing the first exon of the PSP-S gene. The complete mRNA sequence is 775 nucleotides long, including 5'- and 3'- noncoding regions of 80 and 197 nucleotides, respectively, attached to a poly(A) tail of approximately 125 nucleotides. It encodes a preprotein of 166 amino acids, including a prepeptide of 22 amino acids. No overall sequence homology was found between PSP-S and other pancreatic proteins. Some homology with several serine proteases was observed in the COOH-terminal region, however. The mRNA levels of PSP-S, trypsinogen, chymotrypsinogen, and colipase in CCP and control pancreas were compared. PSP-S mRNA was three times lower in CCP than in control, whereas the others were not altered. It was concluded that PSP-S gene expression is specifically reduced in CCP patients.
Giorgi
D
D
Institut National de la Santé et de la Recherche Médicale, Marseille, France.
Bernard
J P
JP
Rouquier
S
S
Iovanna
J
J
Sarles
H
H
Dagorn
J C
JC
D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D015152:Blotting, Northern; D002135:Calcium-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D002908:Chronic Disease; D002919:Chymotrypsinogen / Q000235:genetics; D003089:Colipases / Q000235:genetics; D004247:DNA / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D051847:Lithostathine; D008297:Male; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D009693:Nucleic Acid Hybridization; D010189:Pancreatic Juice / Q000032:analysis; D010195:Pancreatitis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D014362:Trypsinogen / Q000235:genetics
D002135:Calcium-Binding Proteins; D003089:Colipases; D051847:Lithostathine; D009419:Nerve Tissue Proteins; C497617:REG1A protein, human; D012333:RNA, Messenger; D014362:Trypsinogen; D004247:DNA; D002919:Chymotrypsinogen