Modulation of cell-cell and cell-antigen interactions by 1,25-dihydroxyvitamin D3 and vitamin D3 sulfate in vitro: a study on pregnancy lymphocytes and hybridoma cells.
The effect of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) was investigated in single cell cytotoxicity assays, using K-562 target cells. The action of vitamin D3 sulfate (VD3S) in natural cytotoxicity assays as well as its effect on the antigen-specific adherence of hybridoma cells has also been studied. In the single cell cytotoxicity assay 1,25(OH)2D3 dose-dependently and significantly increased the binding of PBMC to target, the number of lysed target cells and NK activity. RU486, a compound known as a potent blocker of progesterone and glucocorticoid receptors, suppressed the effect of 1,25(OH)2D3 in all systems. VD3S dose-dependently decreased the natural cytotoxicity of PBMC and the binding of hybridoma cells to antigen immobilized on plastic surfaces. The results suggest that both 1,25(OH)2D3 and VD3S are potent modulatory agents in cell-cell and cell-antigen interactions.
Najbauer
J
J
Department of Neurology, University Medical School, Pécs, Hungary.
Szekeres-Bartho
J
J
Tigyi
G J
GJ
D000328:Adult; D002117:Calcitriol / Q000494:pharmacology*; D002460:Cell Line; D002478:Cells, Cultured; D002762:Cholecalciferol / Q000494:pharmacology*; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D004963:Estrenes / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D066298:In Vitro Techniques; D007694:Killer Cells, Natural / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism; D015735:Mifepristone; D011247:Pregnancy
D004963:Estrenes; D002762:Cholecalciferol; D015735:Mifepristone; D002117:Calcitriol