Kinetic and inhibitory characteristics of serum angiotensin-converting enzyme from nine mammalian species.
1. Serum angiotensin-converting enzyme activities were obtained from nine mammalian species: rat, mouse, horse, sheep, guinea pig, hamster, rabbit, dog and man. 2. Kinetic constants (Km and Vmax) using hippuryl-L-histidyl-L-leucine as substrate and inhibitory constants (I50 and Ki) for captopril were determined for the serum ACE of each species. 3. There were important differences in the kinetic and inhibitory constants (Kms went from 6.6 mM to 1.21 mM for hamster and guinea pig; I50 ranged from 2100 nM to 3 nM for mouse and sheep) as well as differences in enzyme activity of the different species (values varied from 938 to 13 nmol hippuric acid/ml/min for guinea pig and dog serum).
Ibarra-Rubio
M E
ME
Department of Nephrology and Mineral Metabolism, National Institute of Nutrition Salvador Zubirán, Delegación Tlalpan, D.F. México.
Pena
J C
JC
Pedraza-Chaverri
J
J
D000818:Animals; D002216:Captopril / Q000494:pharmacology; D006224:Cricetinae; D004285:Dogs; D006168:Guinea Pigs; D006736:Horses; D006801:Humans; D007700:Kinetics; D051379:Mice; D007703:Peptidyl-Dipeptidase A / Q000097:blood*; D011817:Rabbits; D051381:Rats; D012756:Sheep; D013045:Species Specificity
D002216:Captopril; D007703:Peptidyl-Dipeptidase A