title: Effect of alpha factor pheromone on the activity of enzymes which catalyze the synthesis and hydrolysis of cell wall structural polymers from Saccharomyces cerevisiae.
abstract: Cells of Saccharomyces cerevisiae of the a mating type treated with alpha factor contain an increased amount of structural polymers (beta-glucans and chitin) in their cell walls and, consequently, exhibit higher glucan synthetase and chitin synthetase activities than untreated cells. However, alpha factor has no detectable effect on the activities of these enzymes when they are assayed, "in vitro", in the presence of the pheromone. On the other hand, the activity of beta-glucanases remains constant during the time that growth of a cells is kept arrested by alpha factor at the G1 phase of the cell division cycle and starts to increase when budding of the cells is reinitiated.
authors.affiliation: Departamento de Microbiología y Biología Celular, Facultad de Farmacia, Universidad de La Laguna, Tenerife, Spain.
authors.forename: T
authors.initials: T
authors.lastname: Ruiz
authors.forename: L
authors.initials: L
authors.lastname: Rodríguez
mesh_terms: D002384:Catalysis; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism; D002686:Chitin / Q000096:biosynthesis*; D002687:Chitin Synthase / Q000378:metabolism; D043326:Glucan 1,3-beta-Glucosidase; D005936:Glucans / Q000096:biosynthesis*; D005964:Glucosyltransferases / Q000378:metabolism; D006868:Hydrolysis; D000072235:Mating Factor; D008565:Membrane Proteins; D010455:Peptides / Q000494:pharmacology*; D010675:Pheromones / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D005936:Glucans; D008565:Membrane Proteins; D010455:Peptides; D010675:Pheromones; D029702:Schizosaccharomyces pombe Proteins; D002686:Chitin; D000072235:Mating Factor; D005964:Glucosyltransferases; D002687:Chitin Synthase; C020570:1,3-beta-glucan synthase; D001617:beta-Glucosidase; D043326:Glucan 1,3-beta-Glucosidase