The urine from patients with peritonitis as a rich source for purifying human acid sphingomyelinase and other lysosomal enzymes.
Milligram amounts of human acid sphingomyelinase (EC 3.1.4.12) were purified to 95% homogeneity using urine from patients with acute peritonitis. The activity of this enzyme is elevated more than 200-times in the urine of these patients. To a lesser extent, levels of some other lysosomal hydrolases are also elevated.
Quintern
L E
LE
Institut für Organische Chemie und Biochemie, Universität Bonn, F.R.G.
Zenk
T S
TS
Sandhoff
K
K
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D005260:Female; D006801:Humans; D007525:Isoelectric Focusing; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D008875:Middle Aged; D010538:Peritonitis / Q000652:urine*; D010727:Phosphoric Diester Hydrolases / Q000302:isolation & purification*; D013108:Sphingomyelin Phosphodiesterase / Q000302:isolation & purification* / Q000652:urine
D010727:Phosphoric Diester Hydrolases; D013108:Sphingomyelin Phosphodiesterase