Localization of human phosphoribosylpyrophosphate synthetase subunit I and II genes (PRPS1 and PRPS2) to different regions of the X chromosome and assignment of two PRPS1-related genes to autosomes.
Complementary DNA clones for phosphoribosylpyrophosphate synthetase subunits I and II (PRS I and PRS II) were used to determine the chromosomal localization of the corresponding human genes. Southern blot analysis of genomic DNAs isolated from human placenta and a panel of human-mouse somatic cell hybrids revealed that the rat PRS I cDNA probe detected at least five human specific DNA segments (23, 20, 14.5, 6.7, and 4.3 kb) in BamHI digests. The 23-, 14.5-, and 6.7-kb DNA segments were detected only if the hybrids contained human chromosome X or translocation chromosome 7p+ (7qter greater than 7p22::Xq21 greater than Xqter), indicating the location of these segments to Xq21-qter (PRPS1). The 20- and 4.3-kb DNA segments did not cosegregate with the other three segments, and spot blot hybridization analysis using flow-sorted human chromosomes indicated that these are the PRPS1-related genes (PRPS1L1 and PRPS1L2) and could be assigned to chromosomes 7 and 9, respectively. The human-specific PRS II cDNA probe revealed a BamHI DNA segment (17 kb), which segregated condordantly with the X chromosome but not with the PRPS1 gene. We surmise that the gene for PRS II (PRPS2) is located at a different region of the X chromosome, namely Xpter-a21.
Taira
M
M
Department of Biochemistry, Chiba University School of Medicine, Japan.
Kudoh
J
J
Minoshima
S
S
Iizasa
T
T
Shimada
H
H
Shimizu
Y
Y
Tatibana
M
M
Shimizu
N
N
D000818:Animals; D015139:Blotting, Southern; D002460:Cell Line; D002874:Chromosome Mapping; D002897:Chromosomes, Human, Pair 7; D002899:Chromosomes, Human, Pair 9; D004247:DNA; D015342:DNA Probes; D005796:Genes; D006801:Humans; D006822:Hybrid Cells; D007621:Karyotyping / Q000379:methods; D051379:Mice; D010770:Phosphotransferases / Q000235:genetics*; D051381:Rats; D012268:Ribose-Phosphate Pyrophosphokinase / Q000235:genetics*; D014960:X Chromosome
D015342:DNA Probes; D004247:DNA; D010770:Phosphotransferases; C000600435:PRPS2 protein, human; D012268:Ribose-Phosphate Pyrophosphokinase