Successful orthotopic heart transplantation in dogs after 24 hours of continuous perfusion with Bretschneider HTK cardioplegic solution.
In 12 dogs the hearts after excision were perfused for 24 hours with Bretschneider HTK cardioplegic solution. Six of these hearts were used only to assess myocardial HEP and ultrastructure during 24 hours of conservation. In the next six dogs orthotopic heart transplantation was performed to evaluate functional outcome after prolonged preservation. After 24 hours of continuous perfusion of the donor heart the ATP level was completely comparable with control, preischemic value. Also ultrastructure of the myocytes was perfectly preserved. All transplanted hearts recovered completely upon reperfusion without a need of inotropic support. Good functional outcome after transplantation was correlated with about 70% of myocardial HEP content and intact ultrastructure of the myocytes. We concluded that continuous perfusion with Bretschneider HTK cardioplegic solution makes successful heart transplantation possible after 24 hours of preservation.
Dyszkiewicz
W
W
Flameng
W
W
Minten
J
J
D000818:Animals; D004285:Dogs; D005947:Glucose; D016027:Heart Transplantation / Q000379:methods*; D008353:Mannitol; D009206:Myocardium / Q000648:ultrastructure; D009926:Organ Preservation / Q000379:methods*; D011189:Potassium Chloride; D011343:Procaine
C039984:Bretschneider cardioplegic solution; D008353:Mannitol; D011343:Procaine; D011189:Potassium Chloride; D005947:Glucose