Properties of interleukin-1 and interferon-gamma receptors in B lymphoid cell line.
We have previously shown that interleukin-1 alpha (IL-1 alpha) and interferon-gamma (IFN-gamma) induce surface IgM expression, stimulate Na+/H+ exchange, and activate protein kinase C in the murine pre-B lymphocyte cell line, 70Z/3. Because the two structurally different lymphokines induce similar effects, in this study we set out to compare the properties of the IL-1 and IFN-gamma surface receptors. In contrast to their similar cellular effects, we found that IL-1 alpha and IFN-gamma receptors have different properties. 70Z/3 have high (100 sites/cell) and low (900 sites/cell) affinity IL-1 receptors with dissociation constants (KD) 6 x 10(-11) and 10(-9) M, respectively. In contrast, IFN-gamma receptors are of one class with a KD of 3 x 10(-10) M and are at a higher number, 8000 sites/cell. After binding to their receptors both IL-1 alpha and IFN-gamma are internalized and intracellularly degraded, but the rate of internalization of IFN-gamma is greater than IL-1 alpha. The effective median concentrations (EC50) of IL-1 alpha- or IFN-gamma-induced surface IgM expression are similar (4-5 x 10(-12) M). However, at this concentration 10-fold more of IFN-gamma than IL-1 alpha molecules are bound per cell. Our studies indicate that structurally different lymphokines can induce similar biological events even though their signaling is mediated by surface receptors whose properties are different.
Bomsztyk
K
K
Department of Medicine, University of Washington, Seattle 98195.
Stanton
T H
TH
Smith
L L
LL
Rachie
N A
NA
Dower
S K
SK
D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology*; D002460:Cell Line; D004705:Endocytosis; D007075:Immunoglobulin M / Q000378:metabolism; D007371:Interferon-gamma / Q000378:metabolism*; D007375:Interleukin-1 / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism; D011971:Receptors, Immunologic / Q000502:physiology*; D017471:Receptors, Interferon; D017472:Receptors, Interleukin-1
D007075:Immunoglobulin M; D007375:Interleukin-1; D011947:Receptors, Antigen, B-Cell; D011971:Receptors, Immunologic; D017471:Receptors, Interferon; D017472:Receptors, Interleukin-1; D007371:Interferon-gamma