Presence and actions of vasopressin-like peptides in the rabbit anterior uvea.
The presence of vasopressin-like immunoreactivity (VP-IR) in the rabbit eye was demonstrated by radioimmunoassay. Trigeminal nerve denervation resulted in a significant and selective decrease in the levels of VP-IR in the iris sphincter muscle and the cornea. The isolated iris sphincter muscle contracted in response to low concentrations of [Arg8]vasopressin (AVP) and related peptides. The V1 vasopressin receptor antagonist, d(CH2)5Tyr(Me)AVP, potently inhibited the contractile responses to AVP. AVP was found to induce an increase in the accumulation of inositol phosphates in the iris sphincter muscle but not in the dilator/ciliary body preparation in vitro. The present investigation demonstrates the presence of VP-IR in the rabbit eye and that this substance may be another sensory nerve-derived mediator acting on specific target sites in the anterior uvea.
Too
H P
HP
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115.
Todd
K
K
Lightman
S L
SL
Horn
A
A
Unger
W G
WG
Hanley
M R
MR
D057911:Angiotensin Receptor Antagonists; D000818:Animals; D001127:Arginine Vasopressin / Q000032:analysis* / Q000494:pharmacology; D002924:Ciliary Body / Q000032:analysis* / Q000378:metabolism; D003315:Cornea / Q000032:analysis / Q000378:metabolism; D003714:Denervation; D005123:Eye / Q000032:analysis / Q000378:metabolism; D007295:Inositol Phosphates / Q000378:metabolism; D007498:Iris / Q000032:analysis* / Q000378:metabolism; D011817:Rabbits; D017483:Receptors, Vasopressin; D014276:Trigeminal Nerve; D014667:Vasopressins / Q000032:analysis* / Q000494:pharmacology
D057911:Angiotensin Receptor Antagonists; D007295:Inositol Phosphates; D017483:Receptors, Vasopressin; D014667:Vasopressins; D001127:Arginine Vasopressin