title: Light microscopical visualization of phosphatase activities demonstrated with the cerium method in resin sections of the kidney with the microwave oven.
abstract: The light microscopically invisible reaction product cerium phosphate in resin sections of rat kidney, that had been incubated for the demonstration of phosphatase activities before embedding, was converted into a visible reaction product by incubation for 10 min at 80 degrees C in alkaline lead citrate in a microwave oven. This method offers the possibility to study phosphatase activities with the cerium method in semithin Epon sections. Furthermore it is a suitable method to select areas with phosphatase activity to be studied with the electron microscope.
authors.affiliation: Centre for Medical Electron Microscopy, University of Groningen, The Netherlands.
authors.forename: D
authors.initials: D
authors.lastname: Kalicharan
authors.forename: C E
authors.initials: CE
authors.lastname: Hulstaert
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D002563:Cerium; D007668:Kidney / Q000166:cytology / Q000201:enzymology*; D007687:Kidney Tubules, Proximal / Q000166:cytology / Q000201:enzymology / Q000648:ultrastructure; D008297:Male; D008871:Microvilli / Q000166:cytology / Q000201:enzymology / Q000648:ultrastructure; D008872:Microwaves; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D002563:Cerium; D010744:Phosphoric Monoester Hydrolases; D000251:Adenosine Triphosphatases