title: Generation of O2- and tyrosine cation-mediated chemiluminescence during the fertilization of sea urchin eggs.
abstract: Sea urchin eggs emit light in the visible region during their fertilization. Judging from the chemiluminescence spectra, one of the excited species generated is considered to have originated from a tyrosine cation radical-mediated reaction. Chemiluminescence probes such as luminol or a cypridina luciferin analog (See text) are useful for detecting the ovoperoxidase +H2O2 reaction associated with membrane hardening and O2- generation, respectively, during fertilization of sea urchin eggs.
authors.affiliation: Research Development Corporation of Japan, Kojinkai Central Hospital, Miyagi, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Takahashi
authors.forename: H
authors.initials: H
authors.lastname: Totsune-Nakano
authors.forename: M
authors.initials: M
authors.lastname: Nakano
authors.forename: S
authors.initials: S
authors.lastname: Mashiko
authors.forename: N
authors.initials: N
authors.lastname: Suzuki
authors.forename: C
authors.initials: C
authors.lastname: Ohma
authors.forename: H
authors.initials: H
authors.lastname: Inaba
mesh_terms: D000818:Animals; D001386:Azides / Q000494:pharmacology; D002374:Catalase / Q000494:pharmacology; D002412:Cations; D005306:Fertilization; D005609:Free Radicals; D006861:Hydrogen Peroxide / Q000378:metabolism; D049449:Luminescence; D008165:Luminol / Q000494:pharmacology; D010063:Ovum / Q000378:metabolism*; D009195:Peroxidase / Q000037:antagonists & inhibitors / Q000378:metabolism; D012617:Sea Urchins; D019810:Sodium Azide; D013053:Spectrophotometry; D013482:Superoxide Dismutase / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism*
chemical_list: D001386:Azides; D002412:Cations; D005609:Free Radicals; D013481:Superoxides; D014443:Tyrosine; D008165:Luminol; D019810:Sodium Azide; D006861:Hydrogen Peroxide; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase