Stimulation of Na-K-Cl cotransport in cultured vascular endothelial cells by atrial natriuretic peptide.
Vascular endothelial cells have been shown to contain atrial natriuretic peptide (ANP)-sensitive Na-K-Cl cotransport system whose activity is regulated by intracellular cGMP levels. Addition of ANP to culture medium stimulated 86Rb+ uptake in bovine endothelial cells with a concomitant increase in cGMP contents. This action of ANP was mimicked by 8-bromo-cGMP and completely diminished by furosemide. These results indicate that ANP selectively activates the Na-K-Cl cotransporter in vascular endothelial cells via cGMP and offer new insight into the physiological significance of endothelial ANP receptors.
Fujita
T
T
Department of Biological Sciences, Tokyo Institute of Technology, Japan.
Hagiwara
H
H
Ohuchi
S
S
Kozuka
M
M
Ishido
M
M
Hirose
S
S
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D001692:Biological Transport / Q000187:drug effects; D002352:Carrier Proteins / Q000378:metabolism*; D002417:Cattle; D002478:Cells, Cultured; D002712:Chlorides / Q000378:metabolism; D006152:Cyclic GMP / Q000502:physiology; D004573:Electrolytes / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000378:metabolism*; D007700:Kinetics; D008565:Membrane Proteins / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D028021:Sodium-Potassium-Chloride Symporters
D002352:Carrier Proteins; D002712:Chlorides; D004573:Electrolytes; D008565:Membrane Proteins; D028021:Sodium-Potassium-Chloride Symporters; D009320:Atrial Natriuretic Factor; D012964:Sodium; D006152:Cyclic GMP; D011188:Potassium