[In vitro study of the influence of donor age, nature of mitogen and exposure to X-rays on the proliferation rate of human lymphocytes].
Proliferation of lymphocytes from donors belonging to 4 age groups (2, 25, 45 and 80 years) and exposed, in vitro, to 0 or 2 Gy of X-rays was studied in cultures stimulated by 3 different mitogens. Our results show that these 3 factors (age, irradiation and mitogen) play a role in the frequencies of cells observed in first, second, third or subsequent cell division in 48 or 72 h cultures.
Deknudt
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Département de Radioprotection, S. C. K.-C. E. N., Mol, Belgique.
Van Autryve
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L
D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects*; D002675:Child, Preschool; D003208:Concanavalin A / Q000494:pharmacology; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D066298:In Vitro Techniques; D037102:Lectins / Q000494:pharmacology; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects / Q000528:radiation effects; D008297:Male; D008875:Middle Aged; D008934:Mitogens / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D037121:Plant Lectins
D037102:Lectins; D008934:Mitogens; D010835:Phytohemagglutinins; D037121:Plant Lectins; C033619:lentil lectin; D003208:Concanavalin A
Etude in vitro de l'influence de l'âge du donneur, de la nature du mitogène et d'une exposition aux rayons X sur la vitesse de prolifération des lymphocytes humains.