Insulin-like growth factor-1 increases the mitogenic response of human peripheral blood lymphocytes to phytohemagglutinin.
Receptors for insulin and insulin-like growth factor-1 (IGF-1) have been demonstrated on activated T-lymphocytes. The question is whether receptors for insulin or IGF-1 have any function in these cells. In this study we demonstrate that the concentration of IGF-1 in commercial samples of fetal calf serum is about 70 times that of insulin. Moreover, antibodies binding IGF-1 reduce responses of human peripheral blood mononuclear cells to PHA by about 50%, whereas antibodies to insulin have no demonstrable effect. These observations suggest that binding of IGF-1 to specific receptors contributes to the proliferative responses of activated T-lymphocytes.
Roldan
A
A
Instituto de Biologia y Medicina Experimental, Buenos Aires, Argentina.
Charreau
E
E
Schillaci
R
R
Eugui
E M
EM
Allison
A C
AC
D000818:Animals; D000906:Antibodies / Q000276:immunology; D002417:Cattle / Q000097:blood; D002455:Cell Division / Q000187:drug effects; D005312:Fetal Blood / Q000502:physiology; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D010835:Phytohemagglutinins / Q000494:pharmacology*; D011956:Receptors, Cell Surface / Q000502:physiology; D017451:Receptors, Somatomedin; D011994:Recombinant Proteins / Q000494:pharmacology; D013002:Somatomedins / Q000494:pharmacology*; D013268:Stimulation, Chemical; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects*
D000906:Antibodies; D010835:Phytohemagglutinins; D011956:Receptors, Cell Surface; D017451:Receptors, Somatomedin; D011994:Recombinant Proteins; D013002:Somatomedins; D007334:Insulin-Like Growth Factor I