Cloning and expression of human leukocyte 5-lipoxygenase.
In conclusion, we have cloned a full-length cDNA for human leukocyte 5-LO from differentiating HL-60 cells. The complete amino acid sequence of 5-LO has been determined from the nucleotide sequence of the cDNA. Some interesting features of the sequence include potential lipid and Ca2+ binding sites and sequence homologies with other lipoxygenases. Human osteosarcoma cells transfected with the 5-LO cDNA expressed 5-LO and LTA4 synthase activities that were indistinguishable from those of the human leukocyte enzyme confirming that the cloned cDNA was the correct gene.
Rouzer
C A
CA
Department of Pharmacology, Merck Frosst Canada, Inc., Pointe Claire-Dorval, Québec.
Bennett
C D
CD
Diehl
R E
RE
Jones
R E
RE
Kargman
S
S
Rands
E
E
Dixon
R A
RA
D000595:Amino Acid Sequence; D001094:Arachidonate 5-Lipoxygenase / Q000096:biosynthesis / Q000235:genetics*; D001091:Arachidonate Lipoxygenases / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular; D005796:Genes; D006801:Humans; D007962:Leukocytes / Q000201:enzymology*; D008969:Molecular Sequence Data; D014162:Transfection
D001091:Arachidonate Lipoxygenases; D001094:Arachidonate 5-Lipoxygenase