Evaluation of two new automated enzymoimmunometric assays for serum follitropin and lutropin.
Two new enzymoimmunoassays for FSH and LH adapted to the ES 600 analyzer (Boehringer Mannheim Diagnostics) were evaluated. The limit of detection was below 0.5 mIU/ml. Precision was excellent, the intra-assay coefficients of variation ranged from 1.2 to 2.2%, and the interassay variations from 2.0 to 2.8%. Both methods compared well with other commercial enzyme immunoassays, with correlation coefficients for FSH and LH of 0.988 and 0.974, respectively. There was no interference on the assays by the rheumatoid factor. The results of an external quality control assured the reliability of the measurements. Preliminary reference values were obtained.
Mirabel
J L
JL
Laboratorio de Bioquímica, Hospital Txagorritxu, Vitoria, Spain.
Zugaza
C
C
Eugui
J
J
D000328:Adult; D002648:Child; D005069:Evaluation Studies as Topic; D005260:Female; D005640:Follicle Stimulating Hormone / Q000032:analysis / Q000097:blood*; D006801:Humans; D007124:Immunoenzyme Techniques; D007986:Luteinizing Hormone / Q000032:analysis / Q000097:blood*; D008297:Male; D015337:Multicenter Studies as Topic; D012016:Reference Values; D012217:Rheumatoid Factor / Q000032:analysis
D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D012217:Rheumatoid Factor