5-HT1B and 5-HT2 serotonin binding sites in cultured Wistar-Kyoto rat aortic smooth muscle cells.
We studied [3H]serotonin [( 3H]5-HT) binding on cultured arterial smooth muscle cells from rat aorta. We found a high and low affinity binding site. Binding to the higher affinity site could be displaced by drugs in an order corresponding most closely with the 5-HT1B subtype, and high affinity [125I]iodo-cyanopindolol binding was also found. We found evidence for a 5-HT2 subtype using [3H]ketanserin binding, with similar results whether specific binding was determined using unlabelled ketanserin, methysergide or mianserin.
Jazayeri
A
A
Department of Psychiatry, University of Texas Medical Branch, Galveston 77550.
Meyer
W J
WJ
Kent
T A
TA
D017371:8-Hydroxy-2-(di-n-propylamino)tetralin; D000818:Animals; D001013:Aorta, Thoracic / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D007457:Iodine Radioisotopes; D020120:Iodocyanopindolol; D007700:Kinetics; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D010869:Pindolol / Q000031:analogs & derivatives; D051381:Rats; D011921:Rats, Inbred WKY; D011985:Receptors, Serotonin / Q000378:metabolism*; D013764:Tetrahydronaphthalenes
D007457:Iodine Radioisotopes; D011985:Receptors, Serotonin; D013764:Tetrahydronaphthalenes; D017371:8-Hydroxy-2-(di-n-propylamino)tetralin; D020120:Iodocyanopindolol; D010869:Pindolol