Characterization of two Ca-ATPases in gill epithelium from the killifish (Fundulus heteroclitus).
1. Two Ca-ATPases in the gill microsomal fraction from the killifish (Fundulus heteroclitus) have been characterized. 2. A (Ca2+ + Mg2+)-ATPase which has a high affinity for Ca2+, requires Mg2+ for activity and may be stimulated by calmodulin. 3. A (Ca2+ + Na+)-ATPase which has a low affinity for Ca2+ requires Na+ for activity, does not require Mg2+ and is probably not stimulated by calmodulin. 4. These enzymes may play a physiological role in killifish calcium regulation.
Paul
M J
MJ
Physiology Department, Tulane Medical School, New Orleans, Louisiana 70112.
Burdick
C J
CJ
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D002118:Calcium / Q000032:analysis / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000302:isolation & purification* / Q000378:metabolism; D003532:Cyprinodontiformes / Q000378:metabolism*; D004848:Epithelium / Q000201:enzymology; D005880:Gills / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000302:isolation & purification*; D007695:Killifishes / Q000378:metabolism*; D008861:Microsomes / Q000201:enzymology; D009994:Osmolar Concentration; D012964:Sodium / Q000494:pharmacology; D013696:Temperature; D014268:Trifluoperazine / Q000494:pharmacology
D007527:Isoenzymes; D014268:Trifluoperazine; D012964:Sodium; D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase; C034688:sodium calcium ATPase; D000252:Calcium-Transporting ATPases; D002118:Calcium