Polyamines inhibit the ATP-dependent proteolytic pathway in rabbit reticulocyte lysates.
Reticulocytes contain a soluble nonlysosomal proteolytic pathway that requires ATP and ubiquitin. Polyamines at physiological concentrations were found to inhibit rapidly the ATP-dependent proteolytic system in reticulocyte lysates; spermidine and putrescine inhibited this process by 26-72% and spermine by 71-96%. Spermine had little effect on the ATP-independent breakdown of oxidant-treated hemoglobin. By fractionating the ATP-dependent system, we show that polyamines inhibit the ATP-dependent degradation of ubiquitin-protein conjugates.
Wajnberg
E F
EF
Department of Animal Sciences, Rutgers University, New Brunswick, NJ 08903.
Fagan
J M
JM
D000255:Adenosine Triphosphate / Q000037:antagonists & inhibitors / Q000502:physiology*; D000818:Animals; D005914:Globins / Q000378:metabolism; D006868:Hydrolysis; D066298:In Vitro Techniques; D009113:Muramidase / Q000378:metabolism; D011073:Polyamines / Q000494:pharmacology*; D011506:Proteins / Q000378:metabolism*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism*; D014452:Ubiquitins / Q000502:physiology
D011073:Polyamines; D011506:Proteins; D014452:Ubiquitins; D000255:Adenosine Triphosphate; D005914:Globins; D009113:Muramidase