Demonstration of surface antigens on bronchoalveolar lavage cells using the immunoalkaline phosphatase method.
The immunoalkaline phosphatase procedure is described as a method for labelling bronchoalveolar lavage cellular specimens with monoclonal antibodies. This method has several advantages over conventional immunofluorescent techniques: it can be performed on cytocentrifuge preparations stored for long periods before staining; cell morphology can be observed in detail in positive and negative cells; the staining is permanent and stable, and, the reaction can be evaluated with a light microscope. Normal values for lymphocyte subpopulations in smokers and nonsmokers are also reported.
Xaubet
A
A
Servicio de Neumología y Hospital Clinic, Barcelona, Spain.
Agusti
C
C
Picado
C
C
Urbano-Ispizua
A
A
Carrión
M
M
Agustí-Vidal
A
A
D000328:Adult; D000469:Alkaline Phosphatase; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis*; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques / Q000592:standards*; D008297:Male; D008875:Middle Aged; D012907:Smoking / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000032:analysis; D050378:T-Lymphocytes, Regulatory / Q000032:analysis
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000469:Alkaline Phosphatase