title: Purification and identification of ADP-ribosylated proteins from bull testis intact nuclei.
abstract: Isolated, intact bull testis nuclei were incubated with [14C] NAD. A large amount of radioactivity was associated to loosely bound chromosomal proteins extracted with 0.35M NaCl and fractionated with trichloroacetic acid. The labelled nuclear proteins included essentially a number of components belonging to the low mobility group. Mg2(+)-catalyzed alkali digestion of radioactive proteins and further analysis demonstrated that the final products were 5'-AMP and phospho-ribosyl-AMP, which arise from the hydrolysis of poly(ADP-ribose).
authors.affiliation: Dipartimento di Chimica Organica e Biologica, Università di Napoli, Italy.
authors.forename: M R
authors.initials: MR
authors.lastname: Faraone-Mennella
authors.forename: A
authors.initials: A
authors.lastname: Raucci
authors.forename: E
authors.initials: E
authors.lastname: Leone
authors.forename: B
authors.initials: B
authors.lastname: Farina
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000032:analysis; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D009687:Nuclear Proteins / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism*; D011064:Poly Adenosine Diphosphate Ribose / Q000378:metabolism*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D013737:Testis / Q000032:analysis* / Q000201:enzymology
chemical_list: D000596:Amino Acids; D009687:Nuclear Proteins; D009702:Nucleoside Diphosphate Sugars; D011064:Poly Adenosine Diphosphate Ribose; D012965:Sodium Chloride; D011065:Poly(ADP-ribose) Polymerases