Phosphorylation of NADPH-cytochrome c reductase in guinea pig peritoneal macrophages stimulated with phorbol myristate acetate.
The plasma membrane-bound NADPH-cytochrome c reductase of guinea pig macrophages (M phi) was found to be phosphorylated when [32P]phosphate-labeled cells were stimulated with 12-phorbol 13-myristate acetate (PMA). The time course of phosphorylation was parallel to that of O2- -generating activity elicited. These results suggest that the reductase participates as a flavoprotein in activation of the respiratory burst NADPH oxidase, when phosphorylated.
Tamoto
K
K
Department of Microbiology, Faculty of Pharmaceutical Sciences, Higashi-Nippon-Gakuen University, Ishikari-Tobetsu, Japan.
Hazeki
K
K
Nochi
H
H
Mori
Y
Y
Koyama
J
J
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D003573:Cytochrome b Group / Q000378:metabolism; D006168:Guinea Pigs; D007163:Immunosorbent Techniques; D007700:Kinetics; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology*; D009251:NADPH-Ferrihemoprotein Reductase / Q000302:isolation & purification / Q000378:metabolism*; D010529:Peritoneal Cavity / Q000166:cytology; D010766:Phosphorylation; D045332:Photosystem II Protein Complex; D011493:Protein Kinase C / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D003573:Cytochrome b Group; D045332:Photosystem II Protein Complex; D013481:Superoxides; D000255:Adenosine Triphosphate; C019313:cytochrome b559; D009251:NADPH-Ferrihemoprotein Reductase; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate