Fluorescent and cytotoxic analogs of corticotropin-releasing factor: probes for studying target cells in heterogeneous populations.
The procedures briefly outlined above described the syntheses of two conjugates of CRF. In each case a molecule with a specific biochemical or physical property was conjugated to an analog of CRF to enable association of the particular property with only CRF-target cells. Thus, by conjugating gelonin or fluorescein to CRF it becomes possible to selectively impair the cellular function of, or fluorescently label, CRF-target cells among mixed populations. These methodologies are still in the early stages of development, and it can be expected that they will become more refined and improved as workers with specialized experience (in synthetic methods, microscopy, cell culture, etc.) continue this work.
Schwartz
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Vale
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D000818:Animals; D002417:Cattle; D003346:Corticotropin-Releasing Hormone / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000378:metabolism / Q000633:toxicity; D005452:Fluoresceins; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D008297:Male; D008856:Microscopy, Fluorescence / Q000379:methods; D010903:Pituitary Gland, Anterior / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D018019:Receptors, Corticotropin-Releasing Hormone; D017981:Receptors, Neurotransmitter / Q000378:metabolism
D005452:Fluoresceins; D007202:Indicators and Reagents; D018019:Receptors, Corticotropin-Releasing Hormone; D017981:Receptors, Neurotransmitter; D003346:Corticotropin-Releasing Hormone