Plasmodium falciparum schizont sonic extracts suppress lymphoproliferative responses to mitogens and antigens in malaria-immune adults.
Cellular immune responses to malaria antigens are suppressed during acute Plasmodium falciparum infection, and evidence from both murine and human studies suggests that parasite-derived factors may be directly immunosuppressive. In this study we have shown that P. falciparum schizont sonic extract will suppress in vitro lymphoproliferative responses to purified malaria antigens and other soluble antigens. The degree of suppression appears to correlate with the level of the lymphoproliferative response to the schizont preparation and is correspondingly more marked in malaria-immune donors than in nonimmune individuals. The effect can be transferred with primed mononuclear cells and is partially abrogated by removal of CD8+ lymphocytes. The suppressive component of the schizont preparation is nondialyzable and partially heat labile and comigrates with hemoglobin-derived proteins in the molecular mass range 10 to 20 kilodaltons.
Riley
E M
EM
Medical Research Council Laboratories, Fajara, Near Banjul, The Gambia.
Jobe
O
O
Blackman
M
M
Whittle
H C
HC
Greenwood
B M
BM
D000328:Adult; D000818:Animals; D000946:Antigens, Fungal / Q000276:immunology*; D000953:Antigens, Protozoan / Q000276:immunology*; D002469:Cell Separation; D006801:Humans; D007113:Immunity, Innate; D007166:Immunosuppressive Agents / Q000502:physiology*; D008213:Lymphocyte Activation / Q000187:drug effects; D008288:Malaria / Q000276:immunology*; D010835:Phytohemagglutinins; D010963:Plasmodium falciparum / Q000254:growth & development / Q000276:immunology*; D013010:Sonication; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D000946:Antigens, Fungal; D000953:Antigens, Protozoan; D007166:Immunosuppressive Agents; D010835:Phytohemagglutinins