Attempt to visualize an endogenous lectin recognizing alpha-L-fucose immunohistochemically in the rabbit oviduct.
An immunohistochemical study was conducted in order to visualize an endogenous lectin, having specificity for alpha-L-fucose residues, in the ampullary and isthmic tracts of the rabbit oviduct. 2 different sequences (S1 and S2) were tested using antilectin UEA I antibody. Reactive sites towards S2 sequence were localized at ciliated cell level in the ampulla; in the isthmus, instead, the staining was present both in ciliated and secretory cells. A role in the sugar immobilization and gamete and zygote protection was postulated for the endogenous lectin evidenced.
Menghi
G
G
Dipartimento di Biologia Cellulare, Università di Camerino, Italy.
Scocco
P
P
Accili
D
D
Bondi
A M
AM
Materazzi
G
G
D000818:Animals; D002923:Cilia / Q000648:ultrastructure; D005187:Fallopian Tubes / Q000166:cytology*; D005260:Female; D005643:Fucose / Q000032:analysis*; D007150:Immunohistochemistry; D037102:Lectins / Q000032:analysis*; D009439:Neuraminidase; D011817:Rabbits; D005644:alpha-L-Fucosidase
D037102:Lectins; D005643:Fucose; D009439:Neuraminidase; D005644:alpha-L-Fucosidase