Cytophotometric study of ATPase activity in brain neurons and gliocytes of paradoxical sleep-deprived rats.
Conditions were chosen for optimal demonstration of ATPases in brain slices by a modified method of Wachstein and Meisel, and the reaction was shown to obey the Bouguer-Beer laws, confirming that ATPase activity can be determined quantitatively in single cells by cytophotometry. In rats in a state of relative physiological rest specific activity of both Na+, K+-ATPase and Mg++-ATPase in gliocytes of the hippocampus and dorsal raphe was found to be considerably higher than in neurons. Deprivation of the paradoxical phase of sleep of the rats for 24 h led to a significant increase in Na+, K+-ATPase activity in hippocampal neurons and to a decrease in its activity in gliocytes of the hippocampus and dorsal nucleus raphe. It is suggested that these changes in Na+, K+-ATPase activity may be due to some extent to a change in excitability of neurons and depolarization of the glia when sleep is disturbed.
Klenikova
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VA
Laboratory of Functional Neurochemistry, I. P. Pavlov Institute of Physiology, Academy of Sciences of the USSR, Leningrad.
Taranova
N P
NP
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000201:enzymology* / Q000502:physiology; D008297:Male; D009457:Neuroglia / Q000201:enzymology* / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D012892:Sleep Deprivation; D012895:Sleep, REM
D000251:Adenosine Triphosphatases