Interferon-gamma is associated with the surface of the human immunodeficiency virus and binds to the gag gene product p17.
Purified HIV-1 antigen preparations produced in cell culture were found to contain interferon-gamma (IFN-gamma). Electron microscopic examination of HIV-1 released by H9 cells, a cell line found to produce IFN-gamma, showed the presence of this molecule on the surface of the virus particle. The HIV-1 protein p17 was found to bind IFN-gamma by a solid-phase radioimmunoassay. The specificity of the reaction was confirmed by Western blot analysis. This finding opens new questions about the biologic role of IFN-gamma itself and of its interaction with HIV.
Caruso
A
A
Institute of Microbiology, University of Brescia, Spedali Civili, Italy.
Pollara
P
P
Foresti
I
I
Bonfanti
C
C
De Francesco
M
M
Gelmi
M
M
Turano
A
A
D001665:Binding Sites; D002460:Cell Line; D015683:Gene Products, gag / Q000378:metabolism*; D015488:HIV Antigens / Q000378:metabolism*; D015497:HIV-1 / Q000276:immunology* / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism*; D008854:Microscopy, Electron; D014764:Viral Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus
D015683:Gene Products, gag; D015488:HIV Antigens; D014764:Viral Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus; C054858:p17 protein, Human Immunodeficiency Virus Type 1; D007371:Interferon-gamma