Epstein-Barr virus episome-based promoter function in human myeloid cells.
Epstein-Barr virus (EBV) episomal replicons offer an expeditious means for amplifying transfected genes in human cells. A panel of EBV episomes was constructed to assess the relative utility of five distinct eukaryotic promoter elements for high level and inducible gene expression in stably transfected human myeloid leukemia cells. The Rous sarcoma virus 3' long terminal repeat (LTR) was most highly suited for EBV episome-based gene expression, whereas the lymphopapilloma virus and the SV40 early regulatory elements exhibited substantially lower activities. Chemically responsive promoter elements, such as the SV40 early, human metallothionein IIA and rat GRP78 gene promoters, retained their inducibility when EBV episome-based. Differences in gene expression obtained with the episomes reflected differential promoter activity rather than significant variations in episome copy numbers per cell. These observations provide guidelines for the optimal design of EBV episomal expression vectors for human expression work.
Hauer
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CA
Institute of Pathology, Case Western Reserve University, Cleveland, OH 44106.
Getty
R R
RR
Tykocinski
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ML
D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002474:Cell-Free System; D015500:Chloramphenicol O-Acetyltransferase; D000091342:Endoplasmic Reticulum Chaperone BiP; D005814:Genes, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D007951:Leukemia, Myeloid / Q000235:genetics; D011401:Promoter Regions, Genetic; D012093:Replicon; D013755:Tetradecanoylphorbol Acetate; D014162:Transfection
D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; D015500:Chloramphenicol O-Acetyltransferase; D013755:Tetradecanoylphorbol Acetate