Identification and isolation from bovine epithelial lens cells of two basic fibroblast growth factor receptors that possess bFGF-enhanced phosphorylation activities.
Cross-linking of bound 125I-basic fibroblast growth factor (bFGF) to bovine epithelial lens cells identified two labelled species whose apparent molecular weights were identical with those of two phosphorylated proteins. The bFGF-stimulated phosphorylation of these proteins was shown to be rapid, suggesting an autophosphorylation process. To demonstrate that the phosphorylated proteins were indeed the bFGF-binding molecules, the two components were purified to homogeneity and their bFGF-binding activity was examined. We conclude that bFGF stimulates the phosphorylation of two receptors of 130 and 160 kDa in bovine epithelial lens cells.
Blanquet
P R
PR
Unité de Recherches Gérontologiques, INSERM U.118, Paris, France.
Patte
C
C
Fayein
N
N
Courtois
Y
Y
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000032:analysis / Q000378:metabolism; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004848:Epithelium / Q000032:analysis / Q000378:metabolism; D005346:Fibroblast Growth Factors / Q000378:metabolism / Q000494:pharmacology*; D007908:Lens, Crystalline / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D008970:Molecular Weight; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000302:isolation & purification / Q000378:metabolism*; D017468:Receptors, Fibroblast Growth Factor; D013388:Succinimides / Q000494:pharmacology
D003432:Cross-Linking Reagents; D011956:Receptors, Cell Surface; D017468:Receptors, Fibroblast Growth Factor; D013388:Succinimides; D005346:Fibroblast Growth Factors; D000255:Adenosine Triphosphate; D011494:Protein Kinases; C019358:disuccinimidyl suberate