Abrogation of spleen macrophage suppressive activity by 15-deoxyspergualin.
We studied the immunomodulatory mechanism of 15-deoxyspergualin (DSG), a novel antitumor agent. To assess this, we used a mixed culture of antigen-primed lymph node cells (LNC) and spleen cells (SPC). In normal or untreated conditions, SPC suppressed the proliferative response of LNC to the antigens. In contrast, SPC from DSG-treated animals did not show any suppressive activity. This suppression was mediated by adherent cells in SPC but the suppression mechanism was recovered when DSG-treated spleen macrophages were reconstituted with control spleen nonadherent cells. These data suggest that DSG abrogates nonspecific suppressive activity of spleen cells by acting on both adherent and nonadherent cells.
Yamamura
T
T
Division of Demyelinating Disease and Aging, National Institute of Neuroscience, Tokyo, Japan.
Tabira
T
T
D000818:Animals; D000903:Antibiotics, Antineoplastic; D002448:Cell Adhesion; D006146:Guanidines / Q000494:pharmacology*; D007108:Immune Tolerance / Q000187:drug effects*; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology; D008198:Lymph Nodes / Q000166:cytology; D008213:Lymphocyte Activation; D008264:Macrophages / Q000187:drug effects*; D011453:Prostaglandins / Q000502:physiology; D051381:Rats; D011917:Rats, Inbred Lew; D011994:Recombinant Proteins / Q000494:pharmacology; D013154:Spleen / Q000166:cytology; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects*
D000903:Antibiotics, Antineoplastic; D006146:Guanidines; D007375:Interleukin-1; D007376:Interleukin-2; D011453:Prostaglandins; D011994:Recombinant Proteins; C037258:gusperimus