Metallothionein induction in rat hippocampal neurons in primary culture.
Primary cultures of hippocampal neurons are important for the study of trace elements in epileptogenesis. We developed a model system for culturing hippocampal neurons on poly-L-lysine in Iscove's modification of Dulbecco's MEM (IMDM) supplemented with K+, D-glucose, glutamine, insulin, p-amino benzoic acid, transferrin, BSA, beta-estradiol, gentamycin, and fungizone. Neurons were identified by histochemical staining for cholinesterase. Zinc at concentrations of 10(-9) to 10(-6) M induced metallothionein in hippocampal neuronal cultures. Maximum metallothionein induction occurred after 48 hrs incubation with zinc.
Thakran
P
P
Department of Pediatrics, University of Nebraska College of Medicine, Omaha 68105.
Leuschen
M P
MP
Ebadi
M
M
D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D002802:Cholinesterases / Q000378:metabolism; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D006624:Hippocampus / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D008297:Male; D008668:Metallothionein / Q000096:biosynthesis*; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009924:Organ Culture Techniques / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains; D015032:Zinc / Q000494:pharmacology*
D003470:Culture Media; D008668:Metallothionein; D002802:Cholinesterases; D015032:Zinc