Replication-defective vectors of reticuloendotheliosis virus transduce exogenous genes into somatic stem cells of the unincubated chicken embryo.
Replication-defective vectors derived from reticuloendotheliosis virus were used to transduce exogenous genes into early somatic stem cells of the chicken embryo. One of these vectors transduced and expressed the chicken growth hormone coding sequence. The helper cell line, C3, was used to generate stocks of vector containing about 10(4) transducing units per ml. Injection of 5- to 20-microliters volumes of vector directly beneath the blastoderm of unincubated chicken embryos led to infection of somatic stem cells. Infected embryos and adults contained unrearranged integrated proviral DNAs. Embryos expressed the transduced chicken growth hormone gene and contained high levels of serum growth hormone. Blood, brain, muscle, testis, and semen contained from individuals injected as embryos contained vector DNA. Replication-defective vectors of the reticuloendotheliosis virus transduced exogenous genes into chicken embryonic stem cells in vivo.
Bosselman
R A
RA
Amgen Inc., Thousand Oaks, California 91320.
Hsu
R Y
RY
Boggs
T
T
Hu
S
S
Bruszewski
J
J
Ou
S
S
Souza
L
L
Kozar
L
L
Martin
F
F
Nicolson
M
M
D000818:Animals; D015139:Blotting, Southern; D015153:Blotting, Western; D002460:Cell Line; D002478:Cells, Cultured; D002642:Chick Embryo; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis*; D005347:Fibroblasts; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D013006:Growth Hormone / Q000032:analysis / Q000235:genetics; D009693:Nucleic Acid Hybridization; D011863:Radioimmunoassay; D001356:Reticuloendotheliosis virus / Q000235:genetics*; D012190:Retroviridae / Q000235:genetics*; D013234:Stem Cells / Q000378:metabolism*; D014161:Transduction, Genetic; D014162:Transfection
D015342:DNA Probes; D004279:DNA, Viral; D013006:Growth Hormone