title: Differential expression of the receptors for epidermal growth factor and insulin in the developing human placenta.
abstract: The detailed cellular distribution of epidermal growth factor (EGF) receptors and insulin receptors during the development of the human placenta was examined. We show that EGF receptors are expressed by villous cytotrophoblast cells in first trimester human placentae. However, where these cells proliferate to form extravillous cytotrophoblast cell columns, there is a dramatic decrease in EGF receptor expression. There is no such differential expression of insulin receptors on this cell population. In contrast, both EGF-and insulin-receptors are present throughout gestation on the microvillous membrane of the terminally differentiated and non-proliferative syncytiotrophoblast although, at term, EGF-but not insulin-receptors are also found on the basolateral membrane of this epithelium. We further show that EGF receptors isolated from first trimester and term human placentae have functional tyrosine kinase activities but differ in their extent of glycosylation. These results suggest that EGF receptors probably play several distinct functional roles in these epithelial cells depending on their proliferative capacity and differentiation status.
authors.affiliation: Department of Biochemistry, University of Bristol Medical School, U.K.
authors.forename: J M
authors.initials: JM
authors.lastname: Tavaré
authors.forename: C H
authors.initials: CH
authors.lastname: Holmes
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000911:Antibodies, Monoclonal; D002454:Cell Differentiation; D002455:Cell Division; D066246:ErbB Receptors / Q000378:metabolism*; D005260:Female; D006026:Glycoside Hydrolases; D006801:Humans; D007124:Immunoenzyme Techniques; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D010761:Phosphorus Radioisotopes; D010920:Placenta / Q000166:cytology / Q000378:metabolism*; D010929:Placentation; D011233:Precipitin Tests; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D011263:Pregnancy Trimester, Third; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011972:Receptor, Insulin / Q000378:metabolism*; D014327:Trophoblasts / Q000166:cytology / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D010761:Phosphorus Radioisotopes; D000255:Adenosine Triphosphate; D066246:ErbB Receptors; D011505:Protein-Tyrosine Kinases; D011972:Receptor, Insulin; D006026:Glycoside Hydrolases; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase