title: Receptors for IgG2b on cells of the mouse metrial gland.
abstract: Single cells prepared from metrial glands of mice killed at days 10, 13 and 17 of pregnancy were assayed for the expression of Fc gamma receptors in a standard rosetting assay using sheep red blood cells sensitised with a mouse monoclonal IgG2b antibody. Rosettes, indicating Fc gamma receptors, were found on both granulated metrial gland (GMG) cells and non-GMG cells, comprising mainly stromal cells, from each stage of pregnancy. Some animals were given an intravenous injection of horseradish peroxidase 2 h before they were killed in order to identify endocytic cells. No GMG cells were found to have endocytosed the horseradish peroxidase. Non-GMG cells which showed endocytic activity all expressed Fc gamma receptors but these receptors were also found on some of the non-GMG cells which had not exhibited endocytosis. The finding of Fc gamma receptors on GMG cells provides further evidence that these cells may be related to NK cells.
authors.affiliation: Department of Biology, Faculty of Science, University of Southampton, U.K.
authors.forename: N M
authors.initials: NM
authors.lastname: Daki
authors.forename: I J
authors.initials: IJ
authors.lastname: Stewart
authors.forename: A E
authors.initials: AE
authors.lastname: Wild
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000378:metabolism*; D004705:Endocytosis; D004912:Erythrocytes; D005260:Female; D005865:Gestational Age; D006735:Horseradish Peroxidase; D008791:Metrial Gland / Q000166:cytology / Q000276:immunology*; D051379:Mice; D011247:Pregnancy; D011961:Receptors, Fc / Q000378:metabolism*; D017452:Receptors, IgG; D012397:Rosette Formation; D012756:Sheep
chemical_list: D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D011961:Receptors, Fc; D017452:Receptors, IgG; D006735:Horseradish Peroxidase