Allogenic induction of thromboplastin synthesis in monocytes and endothelial cells. Biphasic effect of cyclosporin A.
Monocytes and endothelial cells were stimulated in co-culture with allogeneic lymphocytes to produce thromboplastin (TPL). The induction was biphasic, an early response (8-24 h) was greatly augmented by cyclosporin A (CS) (0.5-5 micrograms/ml) whereas the late response (day 3-4) was inhibited. Prednisolone inhibited both responses. Both drugs inhibited lymphocyte proliferation. Interferon-gamma decreased MLC TPL activity but increased thymidine incorporation. CD4+ cells were instrumental in inducing the early TPL peak in monocytes, whereas CD8+ cells decreased the TPL effect. With endothelial cells both T cell classes were equally effective. Conditioned medium from MLC as well as from co-cultures of endothelial cells and lymphocytes induced early TPL synthesis in endothelial cells. Upon allogeneic stimulation monocytes, but not endothelial cells, produced a significant amount of F-VII, most of which was apparently undercarboxylated.
Carlsen
E
E
Surgical Department B, Rikshospitalet, Oslo, Norway.
Gaudernack
G
G
Filion-Myklebust
C
C
Pettersen
K S
KS
Prydz
H
H
D003524:Cyclosporins / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005167:Factor VII / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D007519:Isoantigens / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D011239:Prednisolone / Q000494:pharmacology; D013925:Thromboplastin / Q000096:biosynthesis*
D003524:Cyclosporins; D007519:Isoantigens; D007371:Interferon-gamma; D005167:Factor VII; D013925:Thromboplastin; D011239:Prednisolone