title: Effect of lipid and protein fractions from fetal pulmonary surfactant on prostaglandin E production by a human amnion cell line.
abstract: Discs of amnion and choriodecidua prepared from women delivered at term were incubated with and without surfactant prepared from human amniotic fluid and the output of prostaglandin E (PGE) was estimated by radioimmunoassay. Surfactant stimulated the release of PGE in both tissues. The stimulatory effect was characterized further using cultured human amnion cells. The effect was dose- and time-dependent and required for full expression both protein and lipid components of surfactant. Arachidonate constituted about 3.5% of the fatty acids of fetal surfactant phospholipids. These findings suggest that surfactant is an important source of arachidonate for intrauterine PG synthesis.
authors.affiliation: University of Oxford, Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, Headington, United Kingdom.
authors.forename: A
authors.initials: A
authors.lastname: López Bernal
authors.forename: G E
authors.initials: GE
authors.lastname: Newman
authors.forename: P J
authors.initials: PJ
authors.lastname: Phizackerley
authors.forename: A C
authors.initials: AC
authors.lastname: Turnbull
mesh_terms: D000650:Amnion / Q000166:cytology / Q000378:metabolism*; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D002460:Cell Line; D005591:Chemical Fractionation; D005326:Fetal Proteins / Q000378:metabolism; D006801:Humans; D050356:Lipid Metabolism; D011458:Prostaglandins E / Q000096:biosynthesis*; D011663:Pulmonary Surfactants / Q000378:metabolism*
chemical_list: D001095:Arachidonic Acids; D005326:Fetal Proteins; D011458:Prostaglandins E; D011663:Pulmonary Surfactants; D016718:Arachidonic Acid