[Expression of the Bacillus pumilus chloramphenicol acetyltransferase gene in Bacillus subtilis, achieved by the P-R-promotor of phage lambda].
The possibility of expression of the Bacillus pumilus chloramphenicol acetyltransferase gene (cat) in Bacillus subtilis from the pR promoter of phage lambda has been investigated in this work. For this purpose, the plasmid pPL703 carrying the B. pumilus DNA segment with the cat gene lacking promoter has been combined with the plasmid pBM21 containing the pR promoter. The recombinant plasmid pEL1 is capable of providing the 60 mkg/ml chloramphenicol resistance in Bac. subtilis cells.
Luk'ianov
E V
EV
Chikindas
M L
ML
Stepanov
A I
AI
D001407:Bacillus / Q000235:genetics*; D001412:Bacillus subtilis / Q000235:genetics*; D010582:Bacteriophage lambda / Q000235:genetics*; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D010957:Plasmids; D011401:Promoter Regions, Genetic
D015500:Chloramphenicol O-Acetyltransferase
Ekspressiia gena khloramfenkol-atsetiltransferazy Bacillus pumilus v Bacillus subtilis, osushchestvliaemaia s P-R-promotora faga lambda.