Detection of cytomegalovirus DNA in short term cultures.
Detection of human cytomegalovirus (CMV) by in situ DNA hybridisation six days after incubation of human diploid fibroblasts (ISDH-6) was evaluated prospectively in 205 urine samples, obtained from 57 kidney transplant and 17 bone marrow transplant recipients. The results were compared to those of conventional virus isolation (CVI) and the detection of CMV early antigens after one day of cultivation (EA-1). Of 42 samples positive for CMV by at least one of these methods, 40 (95%) were detected with ISDH-6. Thirty-five (83%) and 34 (81%) positive samples were found with CVI and EA-1, respectively. These data indicate that ISDH-6 is a sensitive method for detection of CMV. It can be used as a rapid and sensitive alternative to CVI in combination with EA-1.
Janssen
H P
HP
Department of Medical Microbiology, University of Nijmegen, The Netherlands.
Meddens
M J
MJ
van Loon
A M
AM
Juffermans
L H
LH
Eickmans-Josten
E C
EC
Quint
W G
WG
D000956:Antigens, Viral / Q000032:analysis; D016026:Bone Marrow Transplantation; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000652:urine; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000032:analysis*; D005347:Fibroblasts; D005455:Fluorescent Antibody Technique; D006801:Humans; D017874:Immediate-Early Proteins; D007108:Immune Tolerance; D016030:Kidney Transplantation; D009693:Nucleic Acid Hybridization; D011237:Predictive Value of Tests; D011446:Prospective Studies
D000956:Antigens, Viral; D004279:DNA, Viral; D017874:Immediate-Early Proteins; C044582:immediate-early proteins, cytomegalovirus