A developmentally regulated gene product from Dictyostelium discoideum shows high homology to human alpha-L-fucosidase.
A cDNA library of poly(A+)-RNA has been prepared from membrane-bound polysomes of Dictyostelium discoideum and screened for clones hybridizing to mRNA species that encode developmentally regulated proteins. The clone investigated in this paper recognizes a 1.8 kb transcript that accumulates strongly between the growth phase and aggregation stage. Stimulation of cells with pulses of cAMP enhances the accumulation. The amino acid sequence derived from a complete cDNA and from a genomic clone displays extensive sequence identity to human liver alpha-L-fucosidase. The D. discoideum DNA sequence encodes a 50.5 kDa polypeptide with a hydrophobic signal peptide at the N-terminus. Antibodies against a synthetic peptide corresponding to amino acids 262-275 of the deduced protein sequence recognize a developmentally regulated 50 kDa protein in D. discoideum that is recovered in the particulate fraction.
Müller-Taubenberger
A
A
Max-Planck-Institut für Biochemie, Martinsried bei München, FRG.
Westphal
M
M
Noegel
A
A
Gerisch
G
G
D000595:Amino Acid Sequence; D001483:Base Sequence; D002462:Cell Membrane / Q000032:analysis; D003001:Cloning, Molecular; D000242:Cyclic AMP / Q000494:pharmacology; D015342:DNA Probes; D004023:Dictyostelium / Q000235:genetics* / Q000254:growth & development; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D011132:Polyribosomes / Q000032:analysis; D012333:RNA, Messenger / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic / Q000187:drug effects; D005644:alpha-L-Fucosidase / Q000235:genetics*
D015342:DNA Probes; D012333:RNA, Messenger; D000242:Cyclic AMP; D005644:alpha-L-Fucosidase