Mobility of the maize suppressor-mutator element in transgenic tobacco cells.
Maize Suppressor-mutator (Spm) transposable elements have been introduced into tobacco cells and a visual assay for Spm activity has been developed using a bacterial beta-glucuronidase gene. The Spm element is mobile in tobacco and can trans-activate excision of a transposition-defective Spm (dSpm) element either from a different site on the same transforming Ti plasmid or from a second plasmid. An Spm element expressed from the stronger cauliflower mosaic virus 35S promoter trans-activates transposition of a dSpm element earlier after its introduction into tobacco cells than an element expressed from its own promoter.
Masson
P
P
Department of Embryology, Carnegie Institution of Washington, Baltimore, MD 21210.
Fedoroff
N V
NV
D001483:Base Sequence; D004251:DNA Transposable Elements; D008969:Molecular Sequence Data; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010944:Plants / Q000235:genetics*; D010947:Plants, Toxic; D010957:Plasmids; D011401:Promoter Regions, Genetic; D013489:Suppression, Genetic; D014026:Nicotiana / Q000235:genetics; D014158:Transcription, Genetic; D003313:Zea mays / Q000235:genetics
D004251:DNA Transposable Elements