title: Direct isolation (without enrichment) of Listeria monocytogenes in milk.
abstract: In the present work we have developed a direct method (without enrichment) for the isolation of listeriae in milk, using the combinated action of SDS (sodium dodecyl sulphate), centrifugation and selective media. It is possible to identify even 0.5 listeriae/ml amongst 7.2 x 10(7) c.f.u. contaminants/ml.
authors.affiliation: Department of Animal Pathology, Microbiology and Immunity, Veterinary Faculty, Madrid, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Dominguez
authors.forename: V
authors.initials: V
authors.lastname: Briones
authors.forename: M
authors.initials: M
authors.lastname: Blanco
authors.forename: J F
authors.initials: JF
authors.lastname: Fernandez-Garayzabal
authors.forename: J L
authors.initials: JL
authors.lastname: Blanco
authors.forename: J A
authors.initials: JA
authors.lastname: Vazquez
authors.forename: G
authors.initials: G
authors.lastname: Suarez
mesh_terms: D000818:Animals; D001386:Azides / Q000494:pharmacology; D001431:Bacteriological Techniques; D002498:Centrifugation; D003470:Culture Media; D005516:Food Microbiology; D008089:Listeria monocytogenes / Q000187:drug effects / Q000302:isolation & purification*; D008892:Milk / Q000382:microbiology*; D019810:Sodium Azide; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology
chemical_list: D001386:Azides; D003470:Culture Media; D012967:Sodium Dodecyl Sulfate; D019810:Sodium Azide