[Possibility of cloning the gene of the regulatory protein of rp1JL-operon of Escherichia coli on the multicopy plasmid pUC supported by convergent transcription induced by the promoter Plac of the vector].
The recombinant plasmids pUC and pNM481 were constructed. The efficiency of P1ac promoter of the vector plasmid pUC and PL10. supporting the transcription of rplJL-rpoBC-operons of Escherichia coli was compared. It was found that the stronger promoter P1ac, due to convergent transcription directed by the promoter, makes possible the cloning on the multicopy plasmid pUC of the DNA fragment (the gene rplJ controlled by its own promoter PL10), coding for the synthesis of the regulatory ribosomal protein L10.
Paton
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Krupskaia
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Zhivolup
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D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D015247:Deoxyribonuclease HindIII; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics*; D005822:Genetic Vectors; D009876:Operon; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
D004262:DNA Restriction Enzymes; D015247:Deoxyribonuclease HindIII
Vozmozhnost' klonirovaniia gena reguliatornogo belka rp1JL-operona Escherichia coli v vysokokopiĭnoĭ plazmide pUC obespechivaetcia konvergentnoĭ transkriptsieĭ, initsiiruemoĭ promotorom R1ac vektora.