[Photodynamic effect of hematoporphyrin on cell organs].
Seminiferous epithelial cells of mouse testis were treated with hematoporphyrin derivative (HPD)-light in vitro. After HPD-light treatment, the changes within cells in early stage of prophase were observed. Examination with EM showed that 30 min after HPD-light treatment, the mitochondria were aggregated and vesiculated, the nucleus showed an irregular outline and a sign of chromatolysis; the other organelles showed no significant changes at this time. The results suggest that both mitochondria and nucleus are susceptible to HPD-light treatment. Three hours after HPD-light treatment, many of multi-nucleated spermatids appeared and they all contained deformed nuclei. This fact suggests that cytokinesis was inhibited by this treatment, and the chromatin of metaphase were also damage.
Yang
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Fu
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Teng
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D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D017324:Hematoporphyrin Derivative; D006414:Hematoporphyrin Photoradiation; D006415:Hematoporphyrins / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D008928:Mitochondria / Q000648:ultrastructure; D015388:Organelles / Q000648:ultrastructure; D010778:Photochemotherapy; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D012670:Seminiferous Epithelium / Q000166:cytology / Q000187:drug effects*; D013737:Testis / Q000187:drug effects*
D006415:Hematoporphyrins; D011838:Radiation-Sensitizing Agents; D017324:Hematoporphyrin Derivative