Kinetics of cell replication in the uterine cervix. VIII. Quantitation of chronologic events in proliferating loci.
The frequency and the topographic distribution of DNA replicating cervical basal squamous cells (as deduced by 3H-thymidine in vivo administration up to 8 hours before sacrifice) was studied in 70,000 basal cells in 70 C57B1 mice. While the number of labelled basal cells/animal was similar in those injected only once, a significantly higher number of labelled basal cells was recorded in animals injected twice or three times. Following one single injection, 30% (8 h previously) and 31% (1 h previously) of the DNA replicating basal cells occurred in pairs or in loci of three, four or more labelled cells. After repeated injections, the rates were 49% for those animals injected twice before sacrifice and 47% for those injected three times. Thus repeated injections resulted in a significantly increased number of loci of three, four or more DNA replicating basal cells when compared to those injected only once. The present results suggest that there is a chronological asynchrony in the DNA replication of proliferating basal cells-loci of the uterine cervix.
Rubio
C A
CA
Department of Pathology, Karolinska Institute, Stockholm, Sweden.
Llatjos
M
M
Nilsson
B
B
D000818:Animals; D002455:Cell Division; D004261:DNA Replication; D004847:Epithelial Cells; D005260:Female; D007700:Kinetics; D051379:Mice; D008810:Mice, Inbred C57BL; D011865:Radioisotope Dilution Technique; D013936:Thymidine / Q000378:metabolism; D014599:Uterus / Q000166:cytology*
D013936:Thymidine