title: Uptake and persistence of ingested antibody in the mosquito Anopheles stephensi.
abstract: A sensitive ELISA was developed to monitor the persistence of a specific antibody, rabbit anti-BSA, in the bloodmeal, haemolymph and tissues of the mosquito Anopheles stephensi Liston. Different concentrations of anti-BSA were fed to female mosquitoes in sheep blood, via a membrane-feeder, and it was found that antibody persisted in the gut as the bloodmeal was digested: concentrations present at 24 h were directly related to those fed. Homogenates of mosquito bodies, from which the intact guts had been removed, were always antibody-positive up to 9 days post-feeding, indicating that undigested antibody had passed through the gut wall into the haemocoele. Haemolymph was extracted from mosquitoes at different times post-feeding, using a microcapillary and manipulator, and antibody was detected in several of the assays. The level of antibodies in the haemolymph 24 h post-feeding was less than half of the level in mosquito heads, indicating removal of antibodies from the haemolymph, perhaps by binding onto haemocoelic tissues. The relevance of these results to the ingestion, survival and fate of antibody against malaria sporozoites is discussed.
authors.affiliation: Department of Zoology, The University, Glasgow.
authors.forename: A M
authors.initials: AM
authors.lastname: Lackie
authors.forename: S
authors.initials: S
authors.lastname: Gavin
mesh_terms: D000818:Animals; D000852:Anopheles / Q000276:immunology*; D000906:Antibodies / Q000032:analysis / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006458:Hemolymph / Q000276:immunology*
chemical_list: D000906:Antibodies