Factors in the liquid portion of stored blood inhibit the proliferative response in mixed lymphocyte cultures.
The transfusion of blood may suppress the immune responses of patients with renal transplants and with malignant disorders. To study the in vitro suppressive effects of banked blood, 4 units of blood were stored in CPDA-1 and ADSOL at 4 degrees C for 14 days. Lymphocytes and plasma or ADSOL supernatants were harvested on Days 0, 4, 7, 10, and 14. Subpopulations of lymphocytes were enumerated by flow cytometry. Recalcified and heat-treated plasma and supernatants from the units of blood were added to mixed lymphocyte cultures (MLC) composed of cells from normal individuals. No significant changes were noted in the proportions of T or B cells from blood stored under these conditions. A 60 +/- 3 percent inhibition in the proliferative response was observed when plasma from CPDA-1 units was added to MLCs (p less than 0.02). Supernatants from ADSOL units demonstrated a 29 +/- 4 percent inhibition (p less than 0.10) of the proliferative response, and this inhibition of response was observed on all 14 days of the study. When appropriate concentrations of dextrose or adenine were added to other MLCs, adenine (at the concentration found in ADSOL) caused a significant inhibition of the proliferative response. This inhibition was not, however, as marked as that observed with recalcified, heat-treated plasma from CPDA-1 units. We conclude that adenine plus some additional factor(s) found in the liquid portion of stored blood inhibits the proliferative response of normal lymphocytes. It is possible that these factors contribute to the immune suppression observed in vivo in some patients who receive blood transfusions.
Vliet
W C
WC
Division of Clinical Pathology, State University of New York Health Science Center, Syracuse.
Dock
N L
NL
Davey
F R
FR
D000225:Adenine / Q000097:blood; D000328:Adult; D000925:Anticoagulants / Q000097:blood; D001786:Blood Glucose; D001793:Blood Preservation; D002951:Citrates / Q000097:blood; D005434:Flow Cytometry; D005947:Glucose; D006801:Humans; D007166:Immunosuppressive Agents / Q000097:blood*; D008213:Lymphocyte Activation / Q000187:drug effects*; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000145:classification; D008297:Male; D008353:Mannitol / Q000097:blood; D008875:Middle Aged; D010641:Phenotype; D010710:Phosphates / Q000097:blood; D012965:Sodium Chloride / Q000097:blood
C037839:ADSOL; D000925:Anticoagulants; D001786:Blood Glucose; C034876:CPDA solutions; D002951:Citrates; D007166:Immunosuppressive Agents; D010710:Phosphates; D008353:Mannitol; D012965:Sodium Chloride; D005947:Glucose; D000225:Adenine