The central segment of herpes simplex virus type 1 glycoprotein C (gC) is not involved in C3b binding: demonstration by using monoclonal antibodies and recombinant gC expressed in Escherichia coli.
Three monoclonal antibodies (MAbs) have been raised against cell membrane-derived herpes simplex virus type 1 glycoprotein C (gC-1). By using different DNA constructs of gC-1 expressed in Escherichia coli the sites recognized by these antibodies could be assigned to a peptide in the more hydrophobic and probably non-glycosylated middle third of the gC-1 molecule. This peptide segment corresponds to a 571 bp segment on the gC-1 gene located between the NcoI and the NruI restriction sites. None of the three MAbs interfered with the binding of the human serum complement component C3b to gC, which has been shown to be rather glycosylation-dependent. Since the data of other groups have suggested that antibodies directed against all regions of gC-1 inhibit C3b binding to gC-1, whereas our results suggest that the central part of gC-1 is not actually involved in C3b-binding activity, it can be inferred that the N- and C-terminal segments are involved in C3b binding by gC-1.
Huemer
H P
HP
Institute for Hygiene, University of Innsbruck, Austria.
Bröker
M
M
Larcher
C
C
Lambris
J D
JD
Dierich
M P
MP
D000818:Animals; D000911:Antibodies, Monoclonal; D001665:Binding Sites; D003179:Complement C3b / Q000276:immunology* / Q000378:metabolism; D004279:DNA, Viral / Q000235:genetics / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D005822:Genetic Vectors; D051379:Mice; D010957:Plasmids; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D018139:Simplexvirus / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D004279:DNA, Viral; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; C038535:glycoprotein gC, herpes simplex virus type 1; D003179:Complement C3b