title: Production and characterization of monoclonal antibodies directed against guinea pig IgG subclasses.
abstract: Monoclonal antibodies directed against guinea pig IgG subclass-specific epitopes have been generated and characterized. Three monoclonal antibodies designated GP1, GP2 and GP3 have been compared to rabbit polyclonal reagents for specificity against purified IgG subclass antigens and for the detection of herpes simplex-specific IgG subclasses, from infected guinea pig, by an indirect ELISA. The monoclonal antibodies were shown to have greater specificity than the corresponding polyclonal reagents.
authors.affiliation: Porton Products, Ltd., PHLS, Salisbury, U.K.
authors.forename: B W
authors.initials: BW
authors.lastname: McBride
authors.forename: D G
authors.initials: DG
authors.lastname: Newell
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis / Q000096:biosynthesis* / Q000502:physiology; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis* / Q000502:physiology; D000914:Antibodies, Viral / Q000032:analysis / Q000096:biosynthesis; D004797:Enzyme-Linked Immunosorbent Assay; D006168:Guinea Pigs; D007074:Immunoglobulin G / Q000008:administration & dosage / Q000145:classification / Q000276:immunology*; D051379:Mice; D018139:Simplexvirus / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D007074:Immunoglobulin G; D014765:Viral Vaccines