The role of IL-1 in the corticotropin-releasing factor and arginine- vasopressin-induced secretion of immunoreactive beta-endorphin by human peripheral blood mononuclear cells.
Corticotropin-releasing factor (CRF) and arginine vasopressin (AVP) stimulate the secretion of beta-endorphin by human PBMC. It is shown here that peripheral blood B cells are responsible for the production of beta-endorphin after culture with CRF and AVP. The presence of CD14+ monocytes is, however, a prerequisite for the enhancing activity of CRF and AVP. The data presented here show that rIL-1 beta can replace CRF and AVP, whereas a mAb directed against IL-1 abrogates the response to CRF and AVP. These results indicate that IL-1 mediates the effect of CRF and AVP on beta-endorphin production by human PBMC.
Kavelaars
A
A
Department of Pediatric Immunology, University Hospital, Utrecht, The Netherlands.
Ballieux
R E
RE
Heijnen
C J
CJ
D000911:Antibodies, Monoclonal / Q000502:physiology; D001127:Arginine Vasopressin / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000502:physiology; D001667:Binding, Competitive; D002469:Cell Separation; D002478:Cells, Cultured; D003346:Corticotropin-Releasing Hormone / Q000494:pharmacology*; D006801:Humans; D007375:Interleukin-1 / Q000276:immunology / Q000502:physiology*; D007963:Leukocytes, Mononuclear / Q000145:classification / Q000276:immunology / Q000502:physiology*; D009000:Monocytes / Q000502:physiology; D013601:T-Lymphocytes / Q000502:physiology; D001615:beta-Endorphin / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D007375:Interleukin-1; D001127:Arginine Vasopressin; D001615:beta-Endorphin; D003346:Corticotropin-Releasing Hormone