title: Retention of ligand binding activity by the extracellular domain of the IL-1 receptor.
abstract: The IL-1R on murine T cells is an 80-kDa cell surface glycoprotein which binds both IL-1 alpha and IL-1 beta. We have recently isolated a cDNA clone encoding this molecule. From the primary sequence mature receptor is predicted to be a 557 residue integral membrane protein with a 319 residue carbohydrate-rich extracellular region. We have constructed a cDNA clone encoding this region of the protein (residues 1 to 316). Expression of this cDNA in HeLa cells leads to secretion of a soluble IL-1 alpha binding protein into the culture medium. Quantitative binding experiments with the truncated receptor show that it possesses IL-1 binding properties which are indistinguishable from those of full length IL-1R. Gel filtration chromatography experiments show that a complex can be formed between a single truncated receptor molecule and a single IL-1 alpha molecule.
authors.affiliation: Immunex Corporation, Seattle, WA 98101.
authors.forename: S K
authors.initials: SK
authors.lastname: Dower
authors.forename: J M
authors.initials: JM
authors.lastname: Wignall
authors.forename: K
authors.initials: K
authors.lastname: Schooley
authors.forename: C J
authors.initials: CJ
authors.lastname: McMahan
authors.forename: J L
authors.initials: JL
authors.lastname: Jackson
authors.forename: K S
authors.initials: KS
authors.lastname: Prickett
authors.forename: S
authors.initials: S
authors.lastname: Lupton
authors.forename: D
authors.initials: D
authors.lastname: Cosman
authors.forename: J E
authors.initials: JE
authors.lastname: Sims
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005109:Extracellular Matrix / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism*; D051379:Mice; D008970:Molecular Weight; D011869:Radioligand Assay; D011971:Receptors, Immunologic / Q000032:analysis / Q000378:metabolism*; D017472:Receptors, Interleukin-1; D011994:Recombinant Proteins / Q000032:analysis / Q000378:metabolism; D012995:Solubility
chemical_list: D007375:Interleukin-1; D011971:Receptors, Immunologic; D017472:Receptors, Interleukin-1; D011994:Recombinant Proteins