A simplified method for the partial purification and enzyme assay of glucosyltransferase from human dental plaque.
A simplified method for the partial purification and enzyme assay of glucosyltransferase from human dental plaque is described. The enzyme obtained has a reasonable specific activity for the assay of soluble and insoluble polysaccharides synthesis. This method may be useful for nonacquainted people with enzymological methods and of great utility in the in vitro evaluation of antiplaque substances.
Pinheiro
C E
CE
Poletto
M I
MI
Pinheiro
C F
CF
D001681:Biological Assay / Q000379:methods; D003773:Dental Plaque / Q000201:enzymology*; D005964:Glucosyltransferases / Q000302:isolation & purification*; D006801:Humans
D005964:Glucosyltransferases