An extended ubiquitin of Dictyostelium is located in the small ribosomal subunit.
According to its cDNA sequence, the product of the DUB1 gene of Dictyostelium discoideum, called ubex52, consists of a ubiquitin monomer with a basic COOH-terminal tail of 52 amino acids that includes a putative zinc finger motif. Antipeptide antibodies raised against the COOH-terminal end of the tail indicated that the ubex52 protein is present in all developmental stages of D. discoideum and that similar proteins with apparent molecular masses of 15 to 17 kDa are found in yeast, wheat germ, Drosophila, and mammals. Subcellular fractionation showed that the D. discoideum and Saccharomyces cerevisiae proteins recognized by the antibodies are associated with the ribosomal fraction. After separation and purification of the 40 and 60 S ribosomal subunits of D. discoideum, the ubex52 protein was exclusively recovered in the small subunit.
Müller-Taubenberger
A
A
Max-Planck-Institut für Biochemie, Martinsried, Federal Republic of Germany.
Graack
H R
HR
Grohmann
L
L
Schleicher
M
M
Gerisch
G
G
D004023:Dictyostelium / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D005800:Genes, Fungal; D008970:Molecular Weight; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification; D012269:Ribosomal Proteins / Q000032:analysis; D012270:Ribosomes / Q000378:metabolism*; D014452:Ubiquitins / Q000235:genetics* / Q000302:isolation & purification
D011993:Recombinant Fusion Proteins; D012269:Ribosomal Proteins; D014452:Ubiquitins