Glucosyltransferase activity in mitochondria. Biosynthesis of glucosyl-phosphoryl-dolichol in inner mitochondrial membranes.
1. Inner mitochondrial membranes are able to transfer [14C]glucose from UDP-[14C]glucose onto dolichylmonophosphate. 2. Synthesis of dolichyl-phosphoryl-glucose takes place only in the presence of exogenous dolichyl-monophosphate loaded into phospholipid vesicles. 3. Neutral phospholipids interact preferentially with the membrane-bound enzyme. The effect of phospholipids is not related to the length of fatty acid chains but a correlation between the activation and the degree of unsaturation of fatty acid chains has been found. 4. This enzyme required divalent cations for activity. Such a requirement might be related to lipid-protein interactions which favour a suitable conformation of glycosyltransferase.
Ardail
D
D
Department of Biochemistry, University of Lyon, Lyon-Sud Medical School, INSERM CNRS U. 189, Oullins, France.
Lerme
F
F
Gateau-Roesch
O
O
Louisot
P
P
Morelis
R
R
D000818:Animals; D002413:Cations, Divalent; D005231:Fatty Acids, Unsaturated / Q000502:physiology; D005947:Glucose / Q000378:metabolism; D005964:Glucosyltransferases / Q000378:metabolism*; D007425:Intracellular Membranes / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D008930:Mitochondria, Liver / Q000201:enzymology* / Q000378:metabolism; D010743:Phospholipids / Q000502:physiology; D011103:Polyisoprenyl Phosphate Monosaccharides / Q000096:biosynthesis*; D011105:Polyisoprenyl Phosphate Sugars / Q000096:biosynthesis*; D011786:Quality Control
D002413:Cations, Divalent; D005231:Fatty Acids, Unsaturated; D010743:Phospholipids; D011103:Polyisoprenyl Phosphate Monosaccharides; D011105:Polyisoprenyl Phosphate Sugars; C002968:dolichol-D-glucosylmonophosphate; D005964:Glucosyltransferases; D005947:Glucose