Interaction retinol-chromatin: an analysis of DNA from vitamin A-treated V79 Chinese hamster cells.
When V79 cells are incubated in the presence of radiolabeled retinol, a small but consistent amount of radioactivity remains associated with nuclear DNA. Chromatographic analysis of enzymatic hydrolysates of DNA shows that no irreversible changes, such as adducts, have taken place on DNA. We present evidence that this radioactive incorporation may occur via a metabolic conversion of retinol, leading mainly to the formation of radiolabeled thymidine, which is then incorporated into newly made DNA. Mutagenic effects by retinol, given at concentrations well above physiological levels, have been also excluded.
Ferrari
N
N
Laboratorio di Biologia Molecolare, Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy.
Rocco
M
M
Aprile
A
A
Fassina
G
G
Vidali
G
G
D000818:Animals; D002352:Carrier Proteins / Q000502:physiology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D009153:Mutagens; D018168:Receptors, Retinoic Acid; D014801:Vitamin A / Q000633:toxicity*
D002352:Carrier Proteins; D009153:Mutagens; D018168:Receptors, Retinoic Acid; D014801:Vitamin A; D004247:DNA