title: Cloning of the cDNA and gene for a human D2 dopamine receptor.
abstract: A clone encoding a human D2 dopamine receptor was isolated from a pituitary cDNA library and sequenced. The deduced protein sequence is 96% identical with that of the cloned rat receptor with one major difference: the human receptor contains an additional 29 amino acids in its putative third cytoplasmic loop. Southern blotting demonstrated the presence of only one human D2 receptor gene. Two overlapping phage containing the gene were isolated and characterized. DNA sequence analysis of these clones showed that the coding sequence is interrupted by six introns and that the additional amino acids present in the human pituitary receptor are encoded by a single exon of 87 base pairs. The involvement of this sequence in alternative splicing and its biological significance are discussed.
authors.affiliation: Vollum Institute for Advanced Biomedical Research, Oregon Health Sciences University, Portland 97201.
authors.forename: D K
authors.initials: DK
authors.lastname: Grandy
authors.forename: M A
authors.initials: MA
authors.lastname: Marchionni
authors.forename: H
authors.initials: H
authors.lastname: Makam
authors.forename: R E
authors.initials: RE
authors.lastname: Stofko
authors.forename: M
authors.initials: M
authors.lastname: Alfano
authors.forename: L
authors.initials: L
authors.lastname: Frothingham
authors.forename: J B
authors.initials: JB
authors.lastname: Fischer
authors.forename: K J
authors.initials: KJ
authors.lastname: Burke-Howie
authors.forename: J R
authors.initials: JR
authors.lastname: Bunzow
authors.forename: A C
authors.initials: AC
authors.lastname: Server
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001667:Binding, Competitive; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003342:Corpus Striatum / Q000378:metabolism; D004247:DNA / Q000235:genetics*; D004294:Domperidone / Q000378:metabolism; D005091:Exons; D015723:Gene Library; D005796:Genes; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D010902:Pituitary Gland / Q000378:metabolism; D051381:Rats; D011954:Receptors, Dopamine / Q000235:genetics* / Q000378:metabolism; D017448:Receptors, Dopamine D2; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
chemical_list: D011954:Receptors, Dopamine; D017448:Receptors, Dopamine D2; D004294:Domperidone; D004247:DNA