An ethidium bromide induced mutant of Neurospora crassa defective in mitochondrial DNA.
Slow growing mutants of Neurospora crassa were obtained by ethidium bromide treatment of the wild type strain. A particular mutant ER-3 showed stopper phenotype accompanied by deficient cytochrome spectra. The mutant showed an altered restriction pattern of the mtDNA which indicated a deletion of 25,000 bp. The phenotype of the ethidium bromide induced mutant ER-3 seem to be related to the loss of several essential genes due to a deletion in its mtDNA.
Niagro
F D
FD
Department of Biological Sciences, University of South Carolina, Columbia 29208.
Mishra
N C
NC
D002872:Chromosome Deletion; D004271:DNA, Fungal / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D004996:Ethidium / Q000494:pharmacology*; D009154:Mutation; D009491:Neurospora / Q000235:genetics*; D009492:Neurospora crassa / Q000187:drug effects / Q000235:genetics*; D010641:Phenotype
D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D004996:Ethidium