Neurogenesis of the peripheral nervous system in Drosophila embryos: DNA replication patterns and cell lineages.
Cell lineages that give rise to the PNS were studied using the thymidine analog 5-bromo-2'-deoxyuridine (BrdU) to visualized DNA replication immunocytochemically. The precursors of the PNS in the body segments of Drosophila embryos replicate their DNA in a spatially and temporally stereotyped pattern. The sequence of DNA replication within developing sensory organs suggests particular lineage relationships of the cells that constitute a sensory organ, i.e., neuron and associated support cells. In embryos that are mutant for the achaete-scute complex or daughterless, in which most or all of the PNS is missing, no BrdU-labeled cells were found in the appropriate regions, suggesting that these PNS precursors either do not form or fail to replicate. Thus, the BrdU technique allows determination as to whether a mutation affects the PNS precursors or terminal differentiation.
Bodmer
R
R
Howard Hughes Medical Institute, Department of Physiology, University of California, San Francisco 94143.
Carretto
R
R
Jan
Y N
YN
D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication; D004331:Drosophila melanogaster / Q000196:embryology* / Q000235:genetics / Q000378:metabolism; D004475:Ectoderm / Q000166:cytology / Q000378:metabolism; D007150:Immunohistochemistry; D009154:Mutation; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D010525:Peripheral Nerves / Q000166:cytology / Q000196:embryology
D004247:DNA; D001973:Bromodeoxyuridine