title: Cloning and expression of human leukocyte 5-lipoxygenase.
abstract: In conclusion, we have cloned a full-length cDNA for human leukocyte 5-LO from differentiating HL-60 cells. The complete amino acid sequence of 5-LO has been determined from the nucleotide sequence of the cDNA. Some interesting features of the sequence include potential lipid and Ca2+ binding sites and sequence homologies with other lipoxygenases. Human osteosarcoma cells transfected with the 5-LO cDNA expressed 5-LO and LTA4 synthase activities that were indistinguishable from those of the human leukocyte enzyme confirming that the cloned cDNA was the correct gene.
authors.affiliation: Department of Pharmacology, Merck Frosst Canada, Inc., Pointe Claire-Dorval, Québec.
authors.forename: C A
authors.initials: CA
authors.lastname: Rouzer
authors.forename: C D
authors.initials: CD
authors.lastname: Bennett
authors.forename: R E
authors.initials: RE
authors.lastname: Diehl
authors.forename: R E
authors.initials: RE
authors.lastname: Jones
authors.forename: S
authors.initials: S
authors.lastname: Kargman
authors.forename: E
authors.initials: E
authors.lastname: Rands
authors.forename: R A
authors.initials: RA
authors.lastname: Dixon
mesh_terms: D000595:Amino Acid Sequence; D001094:Arachidonate 5-Lipoxygenase / Q000096:biosynthesis / Q000235:genetics*; D001091:Arachidonate Lipoxygenases / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular; D005796:Genes; D006801:Humans; D007962:Leukocytes / Q000201:enzymology*; D008969:Molecular Sequence Data; D014162:Transfection
chemical_list: D001091:Arachidonate Lipoxygenases; D001094:Arachidonate 5-Lipoxygenase