Crystallization of porin using short chain phospholipids.
A protein from outer membranes of Escherichia coli K-12, porin OmpF was crystallized either with short acyl chain phospolipids or with various detergents as amphiphiles. In dihexanoyl phosphatidylcholine, crystals greater than 0.3 mm in all dimensions were obtained that diffract X-rays beyond 3.5 A. Crystal morphology and unit cell dimensions were indistinguishable from those of detergent-grown crystals, indicating that the role of the phospholipids used is similar to those of many conventional detergents.
Eiselé
J L
JL
Microbiology Department, Biozentrum University of Basel, Switzerland.
Rosenbusch
J P
JP
D001425:Bacterial Outer Membrane Proteins; D003460:Crystallization; D003902:Detergents; D004926:Escherichia coli; D010713:Phosphatidylcholines; D010743:Phospholipids; D018272:Porins; D014961:X-Ray Diffraction
D001425:Bacterial Outer Membrane Proteins; D003902:Detergents; D010713:Phosphatidylcholines; D010743:Phospholipids; D018272:Porins; C032166:1,2-hexanoylphosphatidylcholine