Rapid diagnosis of cytomegalovirus by indirect immunofluorescence assay with monoclonal antibody F6b in a commercially available kit.
Anti-human cytomegalovirus (CMV) monoclonal antibody (MAb) F6b produced by clone 95/12 was used for the rapid diagnosis (16 to 24 h) of CMV isolates in 308 clinical specimens and compared with classic isolation. MAb F6b gave 100% correlation with isolation. When this MAb was used for direct diagnosis with 212 urine and bronchoalveolar specimens, detection of CMV varied from 50 to 75% compared with viral isolation.
Lucas
G
G
Diagnostics Pasteur, Marnes la Coquette, France.
Seigneurin
J M
JM
Tamalet
J
J
Michelson
S
S
Baccard
M
M
Delagneau
J F
JF
Deletoille
P
P
D000911:Antibodies, Monoclonal; D003587:Cytomegalovirus / Q000302:isolation & purification*; D005455:Fluorescent Antibody Technique; D006801:Humans; D011933:Reagent Kits, Diagnostic
D000911:Antibodies, Monoclonal; D011933:Reagent Kits, Diagnostic