title: Linked scans of peptides and protein digests: amino acid sequence determination of components of complex mixtures.
abstract: Two-sector linked-scan analysis of an unpurified proteolytic digest of a pyruvate decarboxylase enzyme (60,000 Da) has allowed the discovery and assignment of an amino-terminal post-translational modification and processing event. A difference in amino acid sequence from that predicted by a recently published nucleotide sequence has also been found. These results illustrate both the use and considerable potential of linked-scan methods for the analysis of complex biopolymer mixtures.
authors.affiliation: Department of Biochemistry, Imperial College of Science, Technology and Medicine, London, UK.
authors.forename: H R
authors.initials: HR
authors.lastname: Morris
authors.forename: A
authors.initials: A
authors.lastname: Chatterjee
authors.forename: M
authors.initials: M
authors.lastname: Panico
authors.forename: B N
authors.initials: BN
authors.lastname: Green
authors.forename: M A
authors.initials: MA
authors.lastname: Almeida da Silva
authors.forename: B S
authors.initials: BS
authors.lastname: Hartley
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D008969:Molecular Sequence Data; D011767:Pyruvate Decarboxylase / Q000235:genetics*
chemical_list: D011767:Pyruvate Decarboxylase