title: The effect of reduced oxygen tension on progesterone accumulation in rat granulosa cell cultures.
abstract: Studies were carried out on the effect of oxygen tension on progesterone (P) accumulation in rat granulosa cell cultures. At 1-2% oxygen, basal, luteinizing hormone (LH)-stimulated, and follicle stimulating hormone (FSH)-stimulated P accumulations were 20, 18, and 11%, respectively, of P levels at 20% oxygen. Basal P accumulation was also inhibited at 5% oxygen, but LH- and FSH-stimulated P levels were 50% and 40% higher, respectively, than at 20% oxygen. P levels at 10% oxygen were intermediate between those at 5% and 20% oxygen. The inhibitory effect of 1-2% oxygen on P accumulation was reversible: LH-stimulated P accumulation was inhibited in cultures incubated in 1-2% oxygen for 24 h, but rebounded during a subsequent 24 h period in 20% oxygen to the same level as that in cultures maintained continuously in 20% oxygen. We conclude that oxygen tension does influence granulosa cell steroidogenesis in vitro. Changes in blood flow and oxygen delivery to the ovary before and after ovulation could, therefore, effect the pattern of steroidogenesis during this period.
authors.affiliation: Reproductive Sciences Laboratory, University of Miami School of Medicine, FL 33101.
authors.forename: R D
authors.initials: RD
authors.lastname: Koos
authors.forename: M A
authors.initials: MA
authors.lastname: Feiertag
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006107:Granulosa Cells / Q000378:metabolism*; D007773:Lactates / Q000493:pharmacokinetics; D007986:Luteinizing Hormone / Q000494:pharmacology; D010053:Ovary / Q000187:drug effects / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D011374:Progesterone / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D007773:Lactates; D011374:Progesterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D010100:Oxygen