Immunodiagnosis of Schistosomiasis mansoni with APIA (alkaline phosphatase immunoassay).
The previously shown antigenicity of Schistosoma mansoni (JL venezuelan strain) alkaline phosphatase (Mg2+, pH 9.5) allowed its use in an immunodiagnosis assay, that consisted in the immunoadsorption of the enzymatic activity. Protein-A coated polyvinyl plates were used as solid phase to capture IgG from sera of infected human patients. After buffered saline washings, the plates were incubated with an enzyme-rich fraction (a n-butanol aqueous extract of adult worm obtained from pairs). Immunoadsorbed alkaline phosphatase (AP) was revealed by adding rho-nitrophenyl phosphate. Anti-AP antibodies were detected in 93% of coproparasitologically proven S. mansoni-infected venezuelan patients but not in parasite-free control sera and sera from patients infected with parasitosis other than schistosomiasis. The APIA did not correlate with cure but the anti-AP antibody response was progressively reduced after treatment. The use of an AP substrate amplifying system allowed an improvement of the assay sensitivity without loss of specificity. The data suggest that the APIA could be used as a marker of infection by S. mansoni.
Pujol
F H
FH
Laboratorio de Inmunoparasitología, Instituto Venezolano de Investigaciones Científicas, Caracas, Venezuela.
Alarcón de Noya
B
B
Cesari
I M
IM
D000469:Alkaline Phosphatase / Q000097:blood* / Q000276:immunology; D000818:Animals; D004796:Clinical Enzyme Tests; D006801:Humans; D007118:Immunoassay; D007163:Immunosorbent Techniques; D012550:Schistosoma mansoni / Q000201:enzymology* / Q000276:immunology; D012555:Schistosomiasis mansoni / Q000175:diagnosis*
D000469:Alkaline Phosphatase