[HIV envelope proteins are bound by human epidermal Langerhans cells by a binding site which differs from the site on the CD4 molecule, and are internalized by receptor endocytosis].
Langerhans cells (LC) are epidermal dendritic cells which express several surface antigens among them the CD4 antigens. We investigated the fate of HIV envelope glycoproteins (gp 120 and gp 160) incubated with healthy human trypsinized LC in suspension. After trypsin treatment only the epitope for OKT4 appeared to be resistant. In absence of antigenic sites identified by OKT4A, Leu 3a or BL4, LC fixed and internalized gp 120 or gp 160 recombinant HIV proteins. This finding support the hypothesis that there exists at the surface of LC a second molecule which may act as a HIV receptor.
Schmitt
D
D
Unité I.N.S.E.R.M. no. 209, Clinique dermatologique, Hôpital Edouard-Herriot, Lyon.
Dezutter-Dambuyant
C
C
Hanau
D
D
Schmitt
D A
DA
Kolbe
H V
HV
Kieny
M P
MP
Cazenave
J P
JP
Thivolet
J
J
D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D001665:Binding Sites; D004705:Endocytosis; D006678:HIV; D015699:HIV Envelope Protein gp120; D019302:HIV Envelope Protein gp160; D006801:Humans; D007801:Langerhans Cells / Q000276:immunology / Q000378:metabolism*; D015728:Receptors, HIV; D011991:Receptors, Virus / Q000378:metabolism*; D012191:Retroviridae Proteins / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000378:metabolism*
D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D015699:HIV Envelope Protein gp120; D019302:HIV Envelope Protein gp160; D015728:Receptors, HIV; D011991:Receptors, Virus; D012191:Retroviridae Proteins; D014759:Viral Envelope Proteins
Les protéines d'enveloppe du VIH sont fixées par les cellules de Langerhans épidermiques humaines sur un site de fixation qui diffère du site porté par la molécule CD4, et s'internalisent par une "endocytose par récepteurs"