Effects of Tween 80 and sodium fluoride on extracellular glucosyltransferase production and membrane lipids of Streptococcus mutans.
1. Both Tween 80 and sodium fluoride significantly enhanced total extracellular glucosyltransferase activities of Streptococcus mutans. 2. Water-insoluble and water-soluble glucan formation were uniformly increased by Tween 80, whereas fluoride stimulated only water-soluble glucan formation. 3. Elevated glucan formation was due to an increase in enzymes secreted from bacterial cells. 4. Fatty acid composition and phospholipid content in bacterial membrane were changed by Tween 80, although sodium fluoride scarcely showed these changes. 5. Comparative results suggest that modulation of membrane lipids participates in mutansucrase production but not in dextransucrase production of S. mutans.
Sato
M
M
Department of Oral Microbiology, Asahi University School of Dentistry, Gifu, Japan.
Tsuchiya
H
H
Kato
M
M
Yamamoto
K
K
Nakazato
G
G
Takagi
N
N
Namikawa
I
I
D005964:Glucosyltransferases / Q000096:biosynthesis*; D008563:Membrane Lipids / Q000378:metabolism; D011136:Polysorbates / Q000494:pharmacology*; D012969:Sodium Fluoride / Q000494:pharmacology*; D013295:Streptococcus mutans / Q000187:drug effects / Q000201:enzymology*
D008563:Membrane Lipids; D011136:Polysorbates; D012969:Sodium Fluoride; D005964:Glucosyltransferases