title: [Isolation of Corynebacterium diphtheriae from sperm fluid].
abstract: The isolation of toxigenic Corynebacterium diphtheriae from sperm is reported. The organism was identified through the investigation of fluorescence under the UV light, the presence of pirazinecarboxilamidase enzyme (Pyz), in vitro and in vivo and virulence methods (single radial immunodiffusion, cell culture, guinea pig intradermic test). The strain was initially considered nontoxinogenic by single radial immunodiffusion, but its virulence was observed afterwards, when we applied the tests already mentioned. The strain could be considered a "Diphtheroid" without adequate specification.
authors.affiliation: Instituto de Microbiologia, UFRJ, Rio de Janeiro, Brasil.
authors.forename: T L
authors.initials: TL
authors.lastname: Machado
authors.forename: L C
authors.initials: LC
authors.lastname: Formiga
authors.forename: R D
authors.initials: RD
authors.lastname: Machado
authors.forename: R R
authors.initials: RR
authors.lastname: da Silva
mesh_terms: D003353:Corynebacterium diphtheriae / Q000302:isolation & purification* / Q000472:pathogenicity; D003470:Culture Media; D006801:Humans; D005779:Immunodiffusion; D008297:Male; D012661:Semen / Q000382:microbiology*; D014774:Virulence
chemical_list: D003470:Culture Media
vernacular_title: Isolamento de Corynebacterium diphtheriae de Líquido espermático.