title: Viral rel and cellular rel associate with cellular proteins in transformed and normal cells.
abstract: The rel oncogene from the avian reticuloendotheliosis virus strain T is a 59 kd phosphoprotein localized primarily to the cytoplasm of transformed cells. Recently, the v-rel protein was shown to associate with several cellular proteins with molecular weights of 124 kd, 115 kd, and 36 kd. We have analysed the subcellular distribution of v-rel protein complexes after biochemical fractionation of [35S]methionine and [32P]orthophosphate labeled cells. Our results demonstrate that the v-rel protein coprecipitates with a characteristic set of proteins, some of which are distinct to nuclear or cytoplasmic fractions. We also demonstrate that the normal cellular homolog of the viral rel protein, c-rel, coprecipitates with several cellular proteins from normal chick hematopoietic tissue. These cellular proteins have apparent molecular weights similar to those which are coprecipitated with v-rel from cytoplasmic fractions. Our results demonstrate that both v-rel and c-rel interact with a variety of cellular proteins and suggest that this association is important for the function or regulation of the rel protein.
authors.affiliation: Department of Microbiology, State University of New York, Stony Brook 11794.
authors.forename: L E
authors.initials: LE
authors.lastname: Morrison
authors.forename: N
authors.initials: N
authors.lastname: Kabrun
authors.forename: S
authors.initials: S
authors.lastname: Mudri
authors.forename: M J
authors.initials: MJ
authors.lastname: Hayman
authors.forename: P J
authors.initials: PJ
authors.lastname: Enrietto
mesh_terms: D000818:Animals; D002458:Cell Fractionation; D002460:Cell Line; D002461:Cell Line, Transformed; D002467:Cell Nucleus / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D003593:Cytoplasm / Q000378:metabolism; D008970:Molecular Weight; D020572:Oncogene Proteins v-rel; D010766:Phosphorylation; D011233:Precipitin Tests; D011485:Protein Binding; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D020569:Proto-Oncogene Proteins c-rel; D001356:Reticuloendotheliosis virus / Q000235:genetics* / Q000378:metabolism; D012190:Retroviridae / Q000235:genetics*; D012191:Retroviridae Proteins / Q000378:metabolism*
chemical_list: D020572:Oncogene Proteins v-rel; D011518:Proto-Oncogene Proteins; D020569:Proto-Oncogene Proteins c-rel; D012191:Retroviridae Proteins