Variability in messenger RNA levels in human umbilical vein endothelial cells of different lineage and time in culture.
Levels of seven messenger RNA species were compared in human umbilical vein endothelial cells of different lineage and time in culture. Specifically, cells obtained from the American Type Culture Collection (ATCC) and subcultured were compared to early passage cells from cultures produced in our laboratory. Messenger RNA for tissue plasminogen activator, plasminogen activator inhibitor 1, urokinase, and thrombomodulin were expressed at higher levels in the ATCC cells. Thrombospondin, von Willebrand's Factor, and protein S messenger RNA were expressed at higher levels in the cells that we isolated. In addition, in the ATCC cells a shift in the proportion of plasminogen activator inhibitor messenger RNA from the 3.4 to the 2.4 kilobase species was found. We conclude that specific messenger RNA levels can vary considerably between cultured human umbilical vein endothelial cells. The large variation in mRNA levels which we describe has important implications for experiments involving gene expression in cultured endothelium.
Dichek
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Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston 02114.
Quertermous
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D002455:Cell Division; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000235:genetics; D006801:Humans; D010960:Plasminogen Activators / Q000037:antagonists & inhibitors; D015849:Plasminogen Inactivators; D012333:RNA, Messenger / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000235:genetics; D018179:Receptors, Thrombin; D019699:Thrombospondins; D013997:Time Factors; D010959:Tissue Plasminogen Activator / Q000235:genetics; D014471:Umbilical Veins / Q000166:cytology / Q000378:metabolism*; D014568:Urokinase-Type Plasminogen Activator / Q000235:genetics; D014841:von Willebrand Factor / Q000235:genetics
D006023:Glycoproteins; D015849:Plasminogen Inactivators; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D018179:Receptors, Thrombin; D019699:Thrombospondins; D014841:von Willebrand Factor; D010960:Plasminogen Activators; D010959:Tissue Plasminogen Activator; D014568:Urokinase-Type Plasminogen Activator