Cotransfection of nucleic acid segments by Sendai virus envelopes.
It is reported here that Sendai virus envelopes (SVE) can be used to transfect multiple copies of DNA segments of different varieties and size. This capability further increases the usefulness of SVE. In addition, the ability to simultaneously transfect multiple copies of different genome segments promises to be a powerful tool in the field of molecular biology. The simultaneous transfection of NEO gene and cytomegalovirus immediate early antigen gene was successfully done. Sendai virus envelopes (SVE)1 have been used successfully to study carcinogenesis of Epstein-Barr virus (1, 2). SVE have been shown to have a large carrying capacity (3) for the microinjection of macromolecules into target cells. SVE are hollow vesicles constructed from the viral proteins hemagglutinin HN and fusion factor F.
Bartzatt
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Veterinary Basic Science, University of Nebraska, Lincoln 68583-0905.
D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000235:genetics; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D015151:Immunoblotting; D009693:Nucleic Acid Hybridization; D009696:Nucleic Acids / Q000235:genetics*; D010222:Parainfluenza Virus 1, Human / Q000235:genetics*; D014162:Transfection; D014759:Viral Envelope Proteins / Q000235:genetics*
D000911:Antibodies, Monoclonal; D009696:Nucleic Acids; D014759:Viral Envelope Proteins; D004247:DNA