Isolation and sequence determination of rat cardiac natriuretic peptide.
We have isolated a cardiac natriuretic peptide of 5K daltons from the rat atrium and determined its amino acid sequence. The 5K cardiac natriuretic peptide was elucidated to be a 45-amino acid peptide with the sequence of S-Q-D-S-A-F-R-I-Q-E-R-L-R-N-S-K-M-A-H-S-S-S-C-F-G-Q-K-I-D-R-I-G-A-V-S-R- L-G-C-D - G-L-R-L-F by sequencing the native peptide and its lysyl endopeptidase digests. The sequence of this peptide was identical to the amino acid sequence [51-95] of the rat brain natriuretic peptide (BNP) precursor deduced from the cDNA sequence. The 5K cardiac natriuretic peptide, or BNP[51-95], was identified as the major storage and secretory form derived from the BNP precursor in the rat heart.
Kambayashi
Y
Y
Department of Medicine, Kyoto University School of Medicine, Japan.
Nakao
K
K
Itoh
H
H
Hosoda
K
K
Saito
Y
Y
Yamada
T
T
Mukoyama
M
M
Arai
H
H
Shirakami
G
G
Suga
S
S
D000595:Amino Acid Sequence; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000302:isolation & purification* / Q000378:metabolism; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D006325:Heart Atria / Q000032:analysis*; D007158:Immunologic Techniques; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011869:Radioligand Assay; D051381:Rats
D009320:Atrial Natriuretic Factor