The right end of MudI(Ap,lac).
Stable derivatives of the bacteriophage MudI-(Ap,lac) were used to generate operon fusions in S. typhimurium which exhibit a sectoring phenotype with respect to lacZ expression. The Lac- to Lac+ conversion was shown to be the result of small deletions involving the right end of the Mud/I element. DNA sequence analysis of several different fusions revealed that this end of MudI(Ap,lac) contains an assymetric inverted repeat of the attR site found in the wild-type Mu phage. A model is presented which explains how such a structure was formed in the construction of MudI(Ap,lac). In addition, this model explains the observed deletion formation and the Lac- to Lac+ conversion in the sectoring fusions.
Zieg
J
J
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115.
Kolter
R
R
D010583:Bacteriophage mu / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009876:Operon; D010641:Phenotype; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012486:Salmonella typhimurium / Q000235:genetics
D004279:DNA, Viral