title: Transduction and transformation of plasmid DNA in Streptomyces fradiae strains that express different levels of restriction.
abstract: We constructed nonrestricting strains of Streptomyces fradiae blocked in different steps in tylosin biosynthesis. Plasmid transformation frequencies were 10(3)- to 10(4)-fold higher and bacteriophage plating efficiencies were 10(4)- to 10(8)-fold higher in the nonrestricting strains than in the restricting strains. The efficiencies of transduction of plasmid pRHB101 in S. fradiae strains varied by over 1,000-fold, depending on growth conditions, and optimum transduction frequencies were obtained when cells were grown to mid-exponential phase at 39 degrees C. Under these conditions, restricting and nonrestricting strains were transduced at frequencies that differed by only two- to fivefold.
authors.affiliation: Department of Molecular Genetics, Lilly Research Laboratories, Indianapolis, Indiana 46285.
authors.forename: P
authors.initials: P
authors.lastname: Matsushima
authors.forename: M A
authors.initials: MA
authors.lastname: McHenney
authors.forename: R H
authors.initials: RH
authors.lastname: Baltz
mesh_terms: D001435:Bacteriophages / Q000235:genetics; D002455:Cell Division; D003001:Cloning, Molecular / Q000379:methods; D004262:DNA Restriction Enzymes / Q000502:physiology*; D004269:DNA, Bacterial / Q000235:genetics*; D007933:Leucomycins / Q000096:biosynthesis; D010957:Plasmids; D013302:Streptomyces / Q000235:genetics*; D013696:Temperature; D014161:Transduction, Genetic; D014170:Transformation, Genetic; D015645:Tylosin
chemical_list: D004269:DNA, Bacterial; D007933:Leucomycins; D004262:DNA Restriction Enzymes; D015645:Tylosin