title: Identification of papillomaviral DNA sequences in hairless mouse tumours induced by ultraviolet irradiation.
abstract: Papillomas, carcinomas in situ and squamous cell carcinomas were induced using ultraviolet irradiation in the hairless mouse strain Mus musculus HRA/Skh. DNA extracted from biopsies was examined using Mastomys natalensis papillomaviral DNA as a hybridization probe at reduced stringency. Sequences homologous to the probe were detected in 16 of 24 papillomas, five of five carcinomas in situ and six of 38 squamous cell carcinomas. A number of tumour DNAs (16/33) also hybridized with mixed DNAs of human papillomavirus types 11, 13, 16 and 18 at reduced stringency. This suggests a role for the hairless mouse as a laboratory model for the study of the involvement of papillomaviruses in malignant transformations.
authors.affiliation: Department of Microbiology, University of Western Australia, Nedlands.
authors.forename: P A
authors.initials: PA
authors.lastname: Tilbrook
authors.forename: G E
authors.initials: GE
authors.lastname: Greenoak
authors.forename: V E
authors.initials: VE
authors.lastname: Reeve
authors.forename: P J
authors.initials: PJ
authors.lastname: Canfield
authors.forename: L
authors.initials: L
authors.lastname: Gissmann
authors.forename: C H
authors.initials: CH
authors.lastname: Gallagher
authors.forename: J K
authors.initials: JK
authors.lastname: Kulski
mesh_terms: D000818:Animals; D001706:Biopsy; D002278:Carcinoma in Situ / Q000382:microbiology; D002294:Carcinoma, Squamous Cell / Q000382:microbiology; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis*; D004195:Disease Models, Animal; D051379:Mice; D008812:Mice, Hairless; D009693:Nucleic Acid Hybridization; D010212:Papilloma / Q000382:microbiology; D027383:Papillomaviridae / Q000235:genetics*; D012878:Skin Neoplasms / Q000382:microbiology*; D014466:Ultraviolet Rays
chemical_list: D015342:DNA Probes; D004279:DNA, Viral