title: Scrape-loading of Swiss 3T3 cells with ras protein rapidly activates protein kinase C in the absence of phosphoinositide hydrolysis.
abstract: Scrape-loading has been used to analyse the biochemical function of purified p21ras protein. We have shown that scrape-loading oncogenic p21ras into quiescent Swiss 3T3 cells causes morphological transformation of 90% of the cell population within 15 h. Since large numbers of cells can be loaded with p21ras, early induced biochemical changes can be analysed. In this way we have shown that oncogenic p21ras causes rapid activation of protein kinase C five minutes after introduction of protein, but that ras protein fails to stimulate measurable inositol phosphate formation. It appears, therefore, that the stimulation of protein kinase C activity is due to a ras induced increase in diacylglycerol from a source other than inositol phospholipids. Efficient stimulation of DNA synthesis by oncogenic p21ras only occurs in the presence of insulin. This stimulation of DNA synthesis by ras is absolutely dependent on functional protein kinase C activity.
authors.affiliation: Institute of Cancer Research, Royal Cancer Hospital, Chester Beatty Laboratories, London.
authors.forename: J D
authors.initials: JD
authors.lastname: Morris
authors.forename: B
authors.initials: B
authors.lastname: Price
authors.forename: A C
authors.initials: AC
authors.lastname: Lloyd
authors.forename: A J
authors.initials: AJ
authors.lastname: Self
authors.forename: C J
authors.initials: CJ
authors.lastname: Marshall
authors.forename: A
authors.initials: A
authors.lastname: Hall
mesh_terms: D003513:Cycloheximide; D004247:DNA / Q000096:biosynthesis; D005347:Fibroblasts / Q000166:cytology; D006868:Hydrolysis; D007295:Inositol Phosphates / Q000096:biosynthesis; D015689:Oncogene Protein p21(ras); D009856:Oncogene Proteins, Viral / Q000502:physiology*; D010716:Phosphatidylinositols / Q000378:metabolism*; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014162:Transfection / Q000379:methods
chemical_list: D007295:Inositol Phosphates; D009856:Oncogene Proteins, Viral; D010716:Phosphatidylinositols; D011994:Recombinant Proteins; D004247:DNA; D003513:Cycloheximide; D011493:Protein Kinase C; D015689:Oncogene Protein p21(ras); D013755:Tetradecanoylphorbol Acetate