title: [Cell cycle analysis of hybridoma cultures: study by cytofluorimetry].
abstract: An hybridoma cell line cultivated in flasks has been used as a model to study the loss of cell viability in high density cell cultures. Cell cycle analysis by cytofluorimetry has shown that a new hypochromosomic cell population appeared as soon as the viability began to decrease. However there is no evidence that this new population is constituted of dead cells.
authors.affiliation: Unité 284, I.N.S.E.R.M., Vandoeuvre-lès-Nancy.
authors.forename: P
authors.initials: P
authors.lastname: Voisin
authors.forename: V
authors.initials: V
authors.lastname: Eyl
authors.forename: S
authors.initials: S
authors.lastname: Muller
authors.forename: M
authors.initials: M
authors.lastname: Donner
authors.forename: M
authors.initials: M
authors.lastname: Maugras
authors.forename: J F
authors.initials: JF
authors.lastname: Stoltz
mesh_terms: D000818:Animals; D002452:Cell Count; D002453:Cell Cycle; D003470:Culture Media; D005434:Flow Cytometry; D006825:Hybridomas / Q000166:cytology*; D051379:Mice
chemical_list: D003470:Culture Media
vernacular_title: Analyse du cycle cellulaire de cultures d'hybridomes: étude par cytofluorimétrie.