Effects of progesterone and a progesterone antagonist (RU486) on germinal vesicle breakdown in the mouse.
The possibility that ovarian steroids may participate in the inhibition of meiosis has not been rigorously examined. Since progesterone levels are extremely high in follicular fluid prior to ovulation, we tested the possibility that this steroid may be involved in oocyte maturation. To this end, we collected follicular oocytes and cultured them in the presence of dibutyrl cAMP (Bt2), progesterone, and/or the progesterone antagonist RU486 and assessed maturation evidenced by germinal vesicle breakdown (GVBD). Denuded oocytes or cumulus masses collected in the presence of 1 mM Bt2 and subsequently cultured in 25 microM progesterone did not undergo GVBD. However, denuded oocytes and cumulus masses collected in the presence of progesterone and not Bt2 did undergo GVBD (93%). Concentrations of Bt2 (150 microM) that would not inhibit GVBD were inhibitory when used in the presence of progesterone (1-25 microM). Competition experiments using increasing concentrations of the progesterone antagonist RU486 (1-100 microM) did not block the ability of progesterone to enhance the activity of Bt2. We conclude that progesterone alone does not block GVBD; however, in the presence of low concentrations of cAMP it is extremely effective in blocking GVBD. The synergistic activity of progesterone does not appear to be mediated by the progesterone receptor. The data suggest that progesterone and cAMP may operate cooperatively to inhibit meiosis in the ovarian follicle.
Batten
B E
BE
Department of Anatomy, Ohio State University, Columbus 43210.
Roh
S I
SI
Kim
M H
MH
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004963:Estrenes / Q000494:pharmacology*; D005260:Female; D008540:Meiosis / Q000187:drug effects; D051379:Mice; D015735:Mifepristone; D009865:Oocytes / Q000187:drug effects* / Q000502:physiology; D009994:Osmolar Concentration; D011374:Progesterone / Q000037:antagonists & inhibitors / Q000494:pharmacology*
D004963:Estrenes; D015735:Mifepristone; D011374:Progesterone; D003994:Bucladesine