title: Labelling of monoclonal antibodies 10B, 8C, and M2A with indium-111.
abstract: Monoclonal antibodies 10B, 8C and M2A were conjugated with cyclic DTPA anhydride to a substitution level of 1-2 mol DTPA/mol antibody. The DTPA-conjugated antibodies were purified by size-exclusion mini-column chromatography and labelled with [111In]In acetate to a specific activity of 37-74 kBq/micrograms (1-2 microCi/micrograms). The radiochemical purity of the antibody preparation was 76-81%. The immunoreactive fraction of the radiolabelled antibodies ranged from 0.17 to 0.31, significantly lower than that of their radioiodinated analogs.
authors.affiliation: Toronto General Hospital, Division of Nuclear Medicine, Ontario, Canada.
authors.forename: R
authors.initials: R
authors.lastname: Reilly
authors.forename: K
authors.initials: K
authors.lastname: Sheldon
authors.forename: A
authors.initials: A
authors.lastname: Marks
authors.forename: S
authors.initials: S
authors.lastname: Houle
mesh_terms: D000911:Antibodies, Monoclonal; D002460:Cell Line; D005260:Female; D006801:Humans; D007205:Indium Radioisotopes; D007553:Isotope Labeling / Q000379:methods*; D010051:Ovarian Neoplasms / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D007205:Indium Radioisotopes