Phosphofructokinase from porcine heart, liver and erythrocytes.
1. Phosphofructokinase from porcine heart, liver and erythrocytes were purified by affinity chromatographies on Cibacron Blue Sepharose and N6-ATP agarose. 2. Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate revealed that the heart and liver enzymes consist of only one kind of subunit, namely, M and L type subunits, respectively, whereas the erythrocyte enzyme comprises of three kinds of subunits, M, L and C types. 3. Some kinetic and regulatory properties of the enzymes were also measured.
Koyama
T
T
Institute of High Polymer Research, Faculty of Textile Science and Technology, Shishu University, Nagano, Japan.
Ema
T
T
Hashizume
N
N
Hachimori
A
A
Iizuka
E
E
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000201:enzymology*; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology*; D010732:Phosphofructokinase-1 / Q000302:isolation & purification / Q000378:metabolism*; D013552:Swine
D007527:Isoenzymes; D010732:Phosphofructokinase-1