The effect of 3-aminobenzamide on X-ray induction of chromosome aberrations in Down syndrome lymphocytes.
Human lymphocytes from normal and Down syndrome (DS) subjects were examined to determine the effect of 3-aminobenzamide (3AB) on X-ray-induced chromosome aberrations. Lymphocytes were treated with 150 or 300 rad of X-rays in the presence of 3 mM 3AB for various times after irradiation, and then the cells were analyzed for the presence of chromosome aberrations in mitotic cells. 3-Aminobenzamide had no effect on the frequency of chromosome aberrations produced by X-rays in G0 lymphocytes from normal subjects. In contrast, lymphocytes from DS patients displayed an increase in the frequency of chromosome aberrations as a result of treatment with X-rays in the presence of 3AB. These observations indicate that DS lymphocytes are more sensitive to the inhibition of poly(ADP)ribose synthetase than normal lymphocytes.
MacLaren
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University of Texas Medical Branch, Department of Preventive Medicine and Community Health, Galveston 77550.
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Legator
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D001549:Benzamides / Q000494:pharmacology*; D002869:Chromosome Aberrations; D002877:Chromosomes, Human / Q000187:drug effects / Q000528:radiation effects*; D004314:Down Syndrome / Q000235:genetics*; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects*; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D012016:Reference Values; D014965:X-Rays
D001549:Benzamides; D011838:Radiation-Sensitizing Agents; C025160:3-aminobenzamide