title: Studies of human leprosy lesions in situ using suction-induced blisters. 1. Cellular components of new, uncomplicated lesions.
abstract: The cellular contents of blisters induced by suction over new, uncomplicated leprosy lesions, and in the skin of cured, control patients, have been examined with enzyme- and immuno-histochemical staining over a period of 4 days. The total cellularity of the blisters varied over a wide range, not correlated with the type of leprosy. Mononuclear cells predominated at all times studied, with nearly equal percentages of monocytes and T lymphocytes. The T-helper: suppressor ratio was significantly greater in BT than in BL and LL lesions at 48 hr. Suction blisters offer a painless, quantitative, reproducible, multiple-sampling method for obtaining cells from the cutaneous infiltrates of leprosy for phenotyping or functional analysis.
authors.affiliation: Faculty of Medicine, Chiang Mai University, Thailand.
authors.forename: S
authors.initials: S
authors.lastname: Rangdaeng
authors.forename: D M
authors.initials: DM
authors.lastname: Scollard
authors.forename: V
authors.initials: V
authors.lastname: Suriyanon
authors.forename: T
authors.initials: T
authors.lastname: Smith
authors.forename: K
authors.initials: K
authors.lastname: Thamprasert
authors.forename: C
authors.initials: C
authors.lastname: Theetranont
mesh_terms: D001768:Blister / Q000473:pathology*; D002452:Cell Count; D006801:Humans; D007150:Immunohistochemistry; D007918:Leprosy / Q000473:pathology*; D015439:Leprosy, Borderline / Q000473:pathology; D015440:Leprosy, Lepromatous / Q000473:pathology; D015441:Leprosy, Tuberculoid / Q000473:pathology; D009000:Monocytes / Q000473:pathology; D006377:T-Lymphocytes, Helper-Inducer / Q000473:pathology; D050378:T-Lymphocytes, Regulatory / Q000473:pathology