An investigation on lipoperoxidation mechanisms in boar spermatozoa.
Aerobic incubation of washed boar spermatozoa in a heavy metal free medium at 37 degrees C results in a peroxidative breakdown of membrane phospholipids as revealed by malondialdehyde production. In the presence of iron ions, alone and with ascorbate, the amount of malondialdehyde produced increases noticeably. Alkoxy and lipoperoxy radicals are likely involved in these peroxidative processes, while OH. radical does not seem to be essential in the pathway of malondialdehyde formation.
Comaschi
V
V
Institute of Biological Chemistry, School of Medicine, University of Parma, Italy.
Lindner
L
L
Farruggia
G
G
Gesmundo
N
N
Colombi
L
L
Masotti
L
L
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D002374:Catalase / Q000494:pharmacology; D002412:Cations; D004492:Edetic Acid / Q000494:pharmacology; D005290:Ferric Compounds / Q000494:pharmacology; D005296:Ferrous Compounds / Q000494:pharmacology; D006878:Hydroxides / Q000378:metabolism; D017665:Hydroxyl Radical; D007700:Kinetics; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008353:Mannitol / Q000494:pharmacology; D008563:Membrane Lipids / Q000378:metabolism; D010084:Oxidation-Reduction; D013094:Spermatozoa / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000494:pharmacology; D013552:Swine
D002412:Cations; D005290:Ferric Compounds; D005296:Ferrous Compounds; D006878:Hydroxides; D008563:Membrane Lipids; D017665:Hydroxyl Radical; D008353:Mannitol; D008315:Malondialdehyde; D004492:Edetic Acid; D002374:Catalase; D013482:Superoxide Dismutase; D001205:Ascorbic Acid