[Effects of insulin on maltase and N-acetyl-beta-D-glucosaminidase (NAG) activities of serum and kidney in experimental diabetic rats].
The activities of lysosomal maltase in the serum, urine and kidney were determined in rats with diabetes induced by streptozotocin (STZ, 75 mg/kg, i.p.) and compared with the changes in N-acetyl-beta-D-glucosaminidase (NAG) activity. Moreover, effects of insulin on maltase and NAG activities of the serum, urine and kidney in diabetic rats were studied. The following results were obtained: 1) The serum maltase activity within 24 hr after administration of STZ was influenced by insulin secretion. 2) Significant increases in blood urea nitrogen (BUN) levels were observed from the 3rd week after a single administration of STZ. The serum insulin level significantly decreased at 3 weeks after treatment of STZ. In this time, maltase activity in the serum rapidly increased, while the enzyme activity in the kidney decreased considerably. On the other hand, the changes in NAG activities in the serum, urine and kidney after administering STZ were almost similar to those in maltase activities. 3) There were positive relationships between maltase and NAG activities in the serum and urine in diabetic rats, respectively. 4) When lente insulin (2U) was subcutaneously injected once daily for 20 days from 24 hr after administration of STZ, NAG activities in the serum and kidney approached to the control levels. However, maltase activities in the group treated with insulin were significantly higher in the serum and kidney than those in the control group.
Iizuka
Y
Y
Department of Pharmaceutics I, Tohoku College of Pharmacy, Sendai, Japan.
Sakurai
E
E
Hikichi
N
N
Niwa
H
H
D000118:Acetylglucosaminidase / Q000097:blood* / Q000652:urine; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology*; D006596:Hexosaminidases / Q000097:blood*; D007328:Insulin / Q000494:pharmacology*; D007668:Kidney / Q000201:enzymology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013311:Streptozocin; D000520:alpha-Glucosidases / Q000097:blood* / Q000652:urine
D007328:Insulin; D013311:Streptozocin; D006596:Hexosaminidases; D000520:alpha-Glucosidases; D000118:Acetylglucosaminidase