Evaluation of a non-radioactive DNA probe for confirmatory identification of Neisseria gonorrhoeae.
A biotinylated DNA probe combined with a streptavidin-peroxidase complex for the identification of culture isolates of N. gonorrhoeae (Ortho diagnostic systems, Neckargemünd, FRG) was compared with the conventional carbohydrate utilisation test as reference. All 118 strains identified by the reference method as N. gonorrhoeae also gave positive reactions with the DNA hybridisation assay. However, with this test 2 of 23 non-gonococcal Neisseria or Branhamella species were identified as N. gonorrhoeae as well. The study shows that the DNA hybridisation technique can principally be used for the confirmatory identification of N. gonorrhoeae, but since specificity is particularly essential for confirmatory identification, the DNA hybridisation assay evaluated cannot be recommended for routine diagnosis.
Näher
H
H
Universitäts-Hautklinik, Ruprecht-Karls-Universität, Heidelberg.
Kohl
P K
PK
Petzoldt
D
D
D050260:Carbohydrate Metabolism; D003429:Cross Reactions; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D005188:False Negative Reactions; D005189:False Positive Reactions; D006801:Humans; D009344:Neisseria gonorrhoeae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D011237:Predictive Value of Tests
D015342:DNA Probes; D004269:DNA, Bacterial