title: Analysis of the roles of bluetongue virus outer capsid proteins VP2 and VP5 in determination of virus serotype.
abstract: Analyses of reassortant and parental strains of BTV serotypes 3 and 10, in serum neutralization tests, confirmed the major role of outer capsid protein VP2 in determination of virus serotype and its involvement in serum neutralization. However, a reassortant BTV strain (R70), containing protein VP5 derived from BTV 3 and VP2 derived from BTV 10, cross-neutralized with both parental virus strains (BTV 3 and BTV 10). It is concluded that VP5 also plays some part in serotype determination of these virus isolates, as analyzed by serum-neutralization, but its role may be less significant than that of VP2.
authors.affiliation: AFRC Institute of Animal Health, Pirbright Laboratory, England, United Kingdom.
authors.forename: P P
authors.initials: PP
authors.lastname: Mertens
authors.forename: S
authors.initials: S
authors.lastname: Pedley
authors.forename: J
authors.initials: J
authors.lastname: Cowley
authors.forename: J N
authors.initials: JN
authors.lastname: Burroughs
authors.forename: A H
authors.initials: AH
authors.lastname: Corteyn
authors.forename: M H
authors.initials: MH
authors.lastname: Jeggo
authors.forename: D M
authors.initials: DM
authors.lastname: Jennings
authors.forename: B M
authors.initials: BM
authors.lastname: Gorman
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D001820:Bluetongue virus / Q000145:classification / Q000235:genetics / Q000276:immunology*; D002213:Capsid / Q000276:immunology*; D036022:Capsid Proteins; D002460:Cell Line; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D009500:Neutralization Tests; D009693:Nucleic Acid Hybridization; D012330:RNA, Double-Stranded / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D012087:Reoviridae / Q000276:immunology*; D012703:Serotyping
chemical_list: D036022:Capsid Proteins; D012330:RNA, Double-Stranded; D012367:RNA, Viral; C442520:VP2 protein, Bluetongue virus