Immunoreactive evidence of beta-endorphin and methionine-enkephalin-Arg-Gly-Leu in human tooth pulp.
For the first time, beta-endorphin-like immunoreactivity (BE-LI) has been measured in human tooth pulp. Separation of peptides from pulp tissue was achieved by acid extraction followed by chromatographic separation through a Sep-Pak disposable cartridge. Reversed phase high performance liquid chromatographic (RP-HPLC) was performed on the peptide-rich fractions for further peptide separation. Radioreceptor assay (RRA) data of the HPLC fractions was used to construct a profile of opioid-receptor active peptides. Radioimmunoassay (RIA) data provided further information. Following acute mechanical stress, a monotonic decrease in BE-LI concentrations was evident according to a four bicuspid extraction order.
Robinson
Q C
QC
Department of Orthodontics, College of Dentistry, University of Tennessee, Memphis 38163.
Killmar
J T
JT
Desiderio
D M
DM
Harris
E F
EF
Fridland
G
G
D000293:Adolescent; D000328:Adult; D002648:Child; D002851:Chromatography, High Pressure Liquid; D003782:Dental Pulp / Q000032:analysis*; D004744:Enkephalin, Methionine / Q000031:analogs & derivatives* / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D009479:Neuropeptides / Q000032:analysis; D011863:Radioimmunoassay; D011869:Radioligand Assay; D001615:beta-Endorphin / Q000032:analysis*
D009479:Neuropeptides; D004744:Enkephalin, Methionine; D001615:beta-Endorphin