title: The glucose polymer of PC12 cells is susceptible to trypsinization.
abstract: The glucose polymer of PC12 cells (Rasilo, M.-L. and Yamagata, T., 1988, FEBS Letters 227, 191-194 and Rasilo, M.-L. and Yamagata, T., 1988, Journal of Biochemistry, 104, 742-754) was found to be located on the cell surface. The polymer was liberated from the galactose-labeled cells with a trypsin treatment: maximally 65% of the glucose polymer was liberated, compared with 36% of the large glycopeptides. Even when the cells were incubated with the saline about one fourth of the polymer moved into the solution, but less than 8% of the large glycopeptides. Phosphatidyl inositol-specific phospholipase C failed to liberate the polymer.
authors.affiliation: Laboratory of Glycoconjugate Research, Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
authors.forename: M L
authors.initials: ML
authors.lastname: Rasilo
authors.forename: T
authors.initials: T
authors.lastname: Yamagata
mesh_terms: D000310:Adrenal Gland Neoplasms / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D006020:Glycopeptides / Q000378:metabolism; D010673:Pheochromocytoma / Q000378:metabolism*; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010716:Phosphatidylinositols / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D011108:Polymers / Q000378:metabolism*; D051381:Rats; D014357:Trypsin / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D006020:Glycopeptides; D010716:Phosphatidylinositols; D011108:Polymers; D010727:Phosphoric Diester Hydrolases; D014357:Trypsin; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D005947:Glucose; D002118:Calcium