title: Release of cyclooxygenase products from primary cultures of tracheal epithelia of dog and human.
abstract: Release of cyclooxygenase products from primary cultures of dog or human tracheal epithelium was measured by radioimmunoassay. In both species, bradykinin, platelet-activating factor (PAF), and A23187 (a calcium ionophore) caused increases in the rate of release of prostaglandin (PG) E2 and smaller increases in PGF2 alpha, 6-keto-PGF1 alpha, and thromboxane B2 output. Isoproterenol, vasoactive intestinal peptide (VIP), methacholine, and leukotrienes C4 and D4 had no effect on release of these cyclooxygenase products. Gas chromatography-mass spectrometry showed that the ratio of PGE2 to PGD2 released from dog cells by A23187 was 30:1. Short-circuit current across dog cells was stimulated by bradykinin, A23187, PAF, VIP, methacholine, and isoproterenol. Only the responses to bradykinin and A23187 were reduced by pretreatment with indomethacin.
authors.affiliation: Cardiovascular Research Institute, University of California, San Francisco 94143.
authors.forename: J H
authors.initials: JH
authors.lastname: Widdicombe
authors.forename: I F
authors.initials: IF
authors.lastname: Ueki
authors.forename: D
authors.initials: D
authors.lastname: Emery
authors.forename: D
authors.initials: D
authors.lastname: Margolskee
authors.forename: J
authors.initials: J
authors.lastname: Yergey
authors.forename: J A
authors.initials: JA
authors.lastname: Nadel
mesh_terms: D000818:Animals; D001920:Bradykinin / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002478:Cells, Cultured; D015237:Dinoprost / Q000378:metabolism*; D015232:Dinoprostone / Q000378:metabolism*; D004285:Dogs; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology; D007700:Kinetics; D010972:Platelet Activating Factor / Q000494:pharmacology; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D013189:SRS-A / Q000494:pharmacology; D013045:Species Specificity; D013929:Thromboxane B2 / Q000378:metabolism*; D014132:Trachea / Q000378:metabolism / Q000502:physiology*
chemical_list: D010972:Platelet Activating Factor; D013189:SRS-A; D000001:Calcimycin; D013929:Thromboxane B2; D015237:Dinoprost; D011451:Prostaglandin-Endoperoxide Synthases; D015232:Dinoprostone; D007545:Isoproterenol; D001920:Bradykinin