Isolation and enrichment of Ca2+-tolerant myocytes for biochemical experiments from guinea-pig heart.
Isolated myocytes for biochemical experiments must be homogeneous and highly enriched in viable cells. For cardiac myocytes, isolation of Ca2+-tolerant cells in high yield and with good viability has been possible from rat. This paper describes myocyte isolation and enrichment procedures which are effective for several species including guinea-pig and rat. New methods for selection of collagenase and viable cells are presented. Using Ca2+-tolerant myocytes obtained from guinea-pig heart and enriched in viable cells, dihydropyridine binding sites are shown to be accessible only in depolarized cells.
Stemmer
P
P
Department of Pharmacology and Toxicology, Michigan State University, East Lansing 48824.
Akera
T
T
Brody
T M
TM
Rardon
D P
DP
Watanabe
A M
AM
D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002121:Calcium Channel Blockers / Q000378:metabolism; D015220:Calcium Channels; D002470:Cell Survival; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods; D006168:Guinea Pigs; D006321:Heart / Q000187:drug effects*; D008297:Male; D003012:Microbial Collagenase / Q000302:isolation & purification; D008970:Molecular Weight; D009206:Myocardium / Q000166:cytology* / Q000378:metabolism; D011978:Receptors, Nicotinic / Q000378:metabolism
D002121:Calcium Channel Blockers; D015220:Calcium Channels; D011978:Receptors, Nicotinic; D003012:Microbial Collagenase; D002118:Calcium