Linked scans of peptides and protein digests: amino acid sequence determination of components of complex mixtures.
Two-sector linked-scan analysis of an unpurified proteolytic digest of a pyruvate decarboxylase enzyme (60,000 Da) has allowed the discovery and assignment of an amino-terminal post-translational modification and processing event. A difference in amino acid sequence from that predicted by a recently published nucleotide sequence has also been found. These results illustrate both the use and considerable potential of linked-scan methods for the analysis of complex biopolymer mixtures.
Morris
H R
HR
Department of Biochemistry, Imperial College of Science, Technology and Medicine, London, UK.
Chatterjee
A
A
Panico
M
M
Green
B N
BN
Almeida da Silva
M A
MA
Hartley
B S
BS
D000595:Amino Acid Sequence; D001483:Base Sequence; D008969:Molecular Sequence Data; D011767:Pyruvate Decarboxylase / Q000235:genetics*
D011767:Pyruvate Decarboxylase