The U1 RNA-binding site of the U1 small nuclear ribonucleoprotein (snRNP)-associated A protein suggests a similarity with U2 snRNPs.
The site of interaction between human U1 RNA and one of its uniquely associated proteins, A, was examined with in vitro binding assays. The A protein bound directly to stem-loop II of U1 RNA in a region which exhibits sequence similarity to U2 RNA. The similarity with U2 RNA was in a region that has been shown to interact with a U2 RNA-associated protein. The A protein-binding site on U1 RNA overlapped a previously described epitope for an RNA-specific human autoantibody (S. L. Deutscher and J. D. Keene, Proc. Natl. Acad. Sci. USA 85:3299-3303, 1988), supporting the hypothesis that the anti-RNA antibody originated as an anti-idiotypic response to A protein-specific autoantibodies.
Lutz-Freyermuth
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Department of Microbiology and Immunology, Duke University Medical Center, Durham, North Carolina 27710.
Keene
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JD
Lutz-Reyermuth
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D001323:Autoantibodies / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D001667:Binding, Competitive; D015152:Blotting, Northern; D015153:Blotting, Western; D002352:Carrier Proteins / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D005095:Exoribonucleases; D005821:Genetic Techniques; D006367:HeLa Cells; D006801:Humans; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D011233:Precipitin Tests; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins / Q000378:metabolism*; D017411:Ribonucleoproteins, Small Nuclear
D001323:Autoantibodies; D002352:Carrier Proteins; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; D017411:Ribonucleoproteins, Small Nuclear; D005095:Exoribonucleases; C042081:exoribonuclease T