Recombinant interleukin 4 suppresses the production of interferon gamma by human mononuclear cells.
rIL-4 inhibits the production of IFN-gamma by PBMC stimulated with mitogens or allogeneic cells. The suppression is observed at the protein and at the mRNA level; it is dose and time dependent, and it is abolished by a neutralizing mAb to IL-4. It is suggested that the balance between the production of IL-4 and IFN-gamma may be significantly influenced by the chronological order of activation of their respective gene.
Peleman
R
R
Notre-Dame Hospital Research Center, University of Montreal, Quebec, Canada.
Wu
J
J
Fargeas
C
C
Delespesse
G
G
D015152:Blotting, Northern; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000235:genetics; D015847:Interleukin-4 / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008934:Mitogens / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism
D008934:Mitogens; D012333:RNA, Messenger; D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma