Macrophage killing of human papillomavirus type 16-transformed cells.
Papillomaviruses (HPV) are involved in proliferation of epithelial cells and are implicated in several human malignancies. We determined the capability of activated macrophages to exert cytostatic and/or cytolytic activities against HPV type 16 DNA-transformed cells. Both NIH-3T3 and A31-3T3 cells transformed with HPV DNA were susceptible to killing by activated macrophages; however, transformed and nontransformed cells were not susceptible to killing by soluble mediators. The HPV-transformed cells were as resistant as their nontransformed counterparts to recombinant TNF-alpha. These data suggest that the killing of the HPV-16-transformed 3T3 cells by macrophages occurred by a mechanism independent of TNF-alpha.
Denis
M
M
Institute of Parsitology, McGill University, Macdonald College, Ste-Anne de Bellevue, Québec, Canada.
Chadee
K
K
Matlashewski
G J
GJ
D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D003602:Cytotoxicity, Immunologic; D066298:In Vitro Techniques; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D027383:Papillomaviridae; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D014409:Tumor Necrosis Factor-alpha