title: Preparation and certification of standard reference material 1507: 11-nor-delta 9-tetrahydrocannabinol-9-carboxylic acid in freeze-dried urine.
abstract: The National Institute of Standards and Technology (NIST) has prepared and certified SRM 1507, a freeze-dried urine fortified with 11-nor-delta 9-tetrahydrocannabinol-9-carboxylic acid (THC-9-COOH), the major urinary metabolite of marijuana. The certified concentration of 20 +/- 1 ng/mL for the analyte was obtained from the concordant results of analyses of the material by gas chromatography/mass spectrometry (GC/MS) and high-performance liquid chromatography with electrochemical detection (HPLC-EC). Solid-phase extraction was used to prepare the sample for GC/MS analyses, and liquid-liquid extraction was used for the HPLC-EC analyses. The multistep HPLC method was developed at NIST to circumvent interferences from urinary constituents. The results of a round robin test on this material among five Department of Defense laboratories involved in drug testing are reported.
authors.forename: N E
authors.initials: NE
authors.lastname: Craft
authors.forename: G D
authors.initials: GD
authors.lastname: Byrd
authors.forename: L R
authors.initials: LR
authors.lastname: Hilpert
mesh_terms: D002851:Chromatography, High Pressure Liquid; D013759:Dronabinol / Q000031:analogs & derivatives* / Q000592:standards / Q000652:urine; D004563:Electrochemistry; D006801:Humans; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet
chemical_list: C016780:11-nor-delta(9)-tetrahydrocannabinol-9-carboxylic acid; D013759:Dronabinol