The sodium channels of the neuroblastoma x glioma 108 CC 15 hybrid cell change their sensitivity for volatile and local anesthetics upon continuous passage.
We have studied the ion flux through the sodium channels of low passage number (less than 50 p.) and high passage number (greater than 150 p.) neuroblastoma x glioma hybrid cells using [14C] guanidinium and specific neurotoxins to induce channel opening and closing. The sodium channels of low passage number hybrid cells could be opened by veratridine alone, suggesting the presence of voltage dependent channels in agreement with electrophysiological studies reported in the literature. The sodium channels of the high passage number hybrid cells, however, needed the synergistic action of veratridine and scorpion toxin for activation suggesting that these channels are "silent". The [14C] guanidinium ion flux through the sodium channels of the high passage number hybrid cells was inhibited by significantly lower concentrations of the volatile anesthetics (halothane, isoflurane and enflurane) and the local anesthetics (tetracaine and bupivacaine) than the comparable flux through the sodium channels of the low passage number hybrid cells.
Tas
P W
PW
Institute for Virology and Immunology, University of Würzburg, Federal Republic of Germany.
Kress
H G
HG
Koschel
K
K
D000777:Anesthetics / Q000494:pharmacology*; D000818:Animals; D005910:Glioma / Q000378:metabolism*; D019791:Guanidine; D006146:Guanidines; D006221:Halothane; D006825:Hybridomas / Q000187:drug effects / Q000378:metabolism*; D007530:Isoflurane; D009447:Neuroblastoma / Q000378:metabolism*; D009498:Neurotoxins / Q000494:pharmacology; D015222:Sodium Channels / Q000187:drug effects / Q000378:metabolism*; D013748:Tetracaine; D014407:Tumor Cells, Cultured; D014701:Veratridine / Q000494:pharmacology
D000777:Anesthetics; D006146:Guanidines; D009498:Neurotoxins; D015222:Sodium Channels; D013748:Tetracaine; D014701:Veratridine; D007530:Isoflurane; D019791:Guanidine; D006221:Halothane