Detection of hepatitis A virus RNA and capsid antigen in individual cells.
The replication of hepatitis A virus (HAV) RNA and the production of HAV VP1 protein were examined in cultures of BS-C-1 cells under one-step growth conditions by in situ hybridization and immunofluorescence. Individual cells that had undergone active viral RNA replication were detectable at 24 h post-infection. During subsequent days, increasing numbers of cells began replicating viral RNA, so that by seven days post-infection, all cells had accumulated significant amounts of viral RNA. The results show that the protracted replication cycle of HAV in cultured cells represents a slow recruitment of infected cells into a replication mode, rather than an inherently slow virus reproduction in all cells. With the reagents utilized in this study, nucleic acid hybridization was more sensitive than antigen detection by immunofluorescence or immunoblot analysis.
Harmon
S A
SA
Department of Cellular, Viral and Molecular Biology, University of Utah School of Medicine, Salt Lake City 84132.
Summers
D F
DF
Ehrenfeld
E
E
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002213:Capsid / Q000276:immunology*; D002460:Cell Line; D002522:Chlorocebus aethiops; D005455:Fluorescent Antibody Technique; D006507:Hepatovirus / Q000032:analysis* / Q000502:physiology; D007668:Kidney; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis*; D014764:Viral Proteins / Q000032:analysis / Q000276:immunology; D015678:Viral Structural Proteins; D014779:Virus Replication
D000956:Antigens, Viral; D012367:RNA, Viral; D014764:Viral Proteins; D015678:Viral Structural Proteins