The association of the autoantigens of primary biliary cirrhosis with the mitochondrial H+-ATPase--a reassessment.
The claimed association between the M2 autoantigens of primary biliary cirrhosis (PBC) and the mitochondrial H+-ATPase has been re-examined in view of the recent reports that PBC autoantibodies react specifically with the lipoate acetyl transferases of 2-oxo acid dehydrogenases. Study of F0F1-ATPase purified from human and yeast mitochondria, and the comparison between immunoprecipitates obtained with antibodies against the H+-ATPase beta subunit and anti-M2 antibodies of PBC, established that the M2 antigens are not associated with the H+-ATPase complex. The M2 antigens did copurify with a crude bovine heart F1-ATPase preparation, but not with F1-ATPase from yeast, human heart or human liver.
Sudoyo
H
H
Department of Biochemistry, Monash University, Clayton, Victoria, Australia.
Noer
A S
AS
Mackay
I R
IR
Marzuki
S
S
D001324:Autoantigens / Q000276:immunology*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008105:Liver Cirrhosis, Biliary / Q000276:immunology*; D008928:Mitochondria / Q000201:enzymology*; D006180:Proton-Translocating ATPases / Q000276:immunology*; D012441:Saccharomyces cerevisiae
D001324:Autoantigens; D006180:Proton-Translocating ATPases