title: Phosphorylation of NADPH-cytochrome c reductase in guinea pig peritoneal macrophages stimulated with phorbol myristate acetate.
abstract: The plasma membrane-bound NADPH-cytochrome c reductase of guinea pig macrophages (M phi) was found to be phosphorylated when [32P]phosphate-labeled cells were stimulated with 12-phorbol 13-myristate acetate (PMA). The time course of phosphorylation was parallel to that of O2- -generating activity elicited. These results suggest that the reductase participates as a flavoprotein in activation of the respiratory burst NADPH oxidase, when phosphorylated.
authors.affiliation: Department of Microbiology, Faculty of Pharmaceutical Sciences, Higashi-Nippon-Gakuen University, Ishikari-Tobetsu, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Tamoto
authors.forename: K
authors.initials: K
authors.lastname: Hazeki
authors.forename: H
authors.initials: H
authors.lastname: Nochi
authors.forename: Y
authors.initials: Y
authors.lastname: Mori
authors.forename: J
authors.initials: J
authors.lastname: Koyama
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D003573:Cytochrome b Group / Q000378:metabolism; D006168:Guinea Pigs; D007163:Immunosorbent Techniques; D007700:Kinetics; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology*; D009251:NADPH-Ferrihemoprotein Reductase / Q000302:isolation & purification / Q000378:metabolism*; D010529:Peritoneal Cavity / Q000166:cytology; D010766:Phosphorylation; D045332:Photosystem II Protein Complex; D011493:Protein Kinase C / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
chemical_list: D003573:Cytochrome b Group; D045332:Photosystem II Protein Complex; D013481:Superoxides; D000255:Adenosine Triphosphate; C019313:cytochrome b559; D009251:NADPH-Ferrihemoprotein Reductase; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate