Inhibition of experimental metastasis of murine fibrosarcoma cells by oligopeptide analogues to the fibronectin cell-binding site.
We have analyzed the effect of the synthetic oligopeptides Gly-Arg-Gly-Asp-Ser-Pro (GRGDSP) and Gly-Arg-Gly-Glu-Ser-Pro (GRGESP), analogues to the fibronectin cell-binding sequence, on the formation of experimental lung metastasis. SR-BALB were injected alone or in conjunction with GRGDSP or GRGESP in the tail vein of BALB/c mice. Co-injection with GRGESP reduced by approximately 70% the number of metastatic colonies per mouse. However, co-injection with the closely related peptide GRGDSP, containing the conservative substitution Glu/Asp, did not affect metastatic behavior. GRGESP peptide anti-metastatic activity was not due to a toxic effect on tumor cells or on mice. In vitro adhesion assays testing for a possible effect of the peptide on cell-matrix interactions indicated that the GRGESP peptide did not affect cell adhesion to the matrix proteins tested.
Bretti
S
S
Department of Genetics, University of Turin, Italy.
Neri
P
P
Lozzi
L
L
Rustici
M
M
Comoglio
P
P
Giancotti
F
F
Tarone
G
G
D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D005354:Fibrosarcoma / Q000473:pathology*; D008175:Lung Neoplasms / Q000556:secondary; D051379:Mice; D008807:Mice, Inbred BALB C; D009362:Neoplasm Metastasis / Q000517:prevention & control; D009368:Neoplasm Transplantation; D009842:Oligopeptides / Q000494:pharmacology*; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic / Q000032:analysis*
D009842:Oligopeptides; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic; C058161:glycyl-arginyl-glycyl-aspartyl-seryl-proline; C058347:glycyl-arginyl-glycyl-glutamyl-seryl-proline