Ontogenetic expression of peptidyl-glycine alpha-amidating monooxygenase mRNA in the rat pancreas.
Qualitative and quantitative expression of m.RNA coding for Peptidyl-Glycine alpha-Amidating Monooxygenase (PAM) in the developing rat pancreas was investigated by Northern and dot blot hybridization, with a bovine PAM c.DNA probe (0.7 kb fragment). A specific hybridization signal was evidenced for a 3.7 kb m.RNA species. Measurement of PAM m. RNA rate during the rat pancreas ontogenesis revealed a biphasic profile which appeared corelated with that of gastrin and TRH m.RNA respectively. On the other hand, streptozotocin-treatment resulted in a 50% decrease of PAM m.RNA levels.
Maltese
J Y
JY
Laboratoire de Neuroendocrinologie Expérimentale, INSERM U, 297, Faculté de Médecine Nord, Marseille, France.
Giraud
P
P
Kowalski
C
C
Ouafik
L H
LH
Salers
P
P
Pelen
F
F
Oliver
C
C
D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D015152:Blotting, Northern; D015342:DNA Probes; D006899:Mixed Function Oxygenases; D009097:Multienzyme Complexes; D009693:Nucleic Acid Hybridization; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000235:genetics*; D010179:Pancreas / Q000201:enzymology / Q000254:growth & development*; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D051381:Rats; D011919:Rats, Inbred Strains; D013311:Streptozocin / Q000494:pharmacology
D015342:DNA Probes; D009097:Multienzyme Complexes; D012333:RNA, Messenger; D013311:Streptozocin; D006899:Mixed Function Oxygenases; C035189:peptidylglycine monooxygenase; D000587:Oxidoreductases Acting on CH-NH Group Donors