title: Nucleotide sequence of the gene encoding the active-site serine beta-lactamase from Actinomadura R39.
abstract: The gene encoding the extracellular, active-site serine beta-lactamase of Actinomadura R39, previously cloned into Streptomyces lividans, has the information for the synthesis of a 304 amino acid protein, the amino terminal region of which has the characteristic features of a signal peptide. The Actinomadura R39 beta-lactamase is another member of the class A beta-lactamases. In particular, it shows high homology with the beta-lactamase of Bacillus licheniformis.
authors.affiliation: Service de Microbiologie, Université de Liège, Institut de Chimie, Sart Tilman, Belgium.
authors.forename: S
authors.initials: S
authors.lastname: Houba
authors.forename: S
authors.initials: S
authors.lastname: Willem
authors.forename: C
authors.initials: C
authors.lastname: Duez
authors.forename: C
authors.initials: C
authors.lastname: Molitor
authors.forename: J
authors.initials: J
authors.lastname: Dusart
authors.forename: J M
authors.initials: JM
authors.lastname: Frère
authors.forename: J M
authors.initials: JM
authors.lastname: Ghuysen
mesh_terms: D000192:Actinomycetales / Q000201:enzymology / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010957:Plasmids; D015183:Restriction Mapping; D012694:Serine; D013302:Streptomyces / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D012694:Serine; D001618:beta-Lactamases