Isolation and restriction map of the V-J interval of the human T cell receptor gamma chain locus.
The human T cell receptor gamma chain locus encodes the immunoglobulin-like gamma chain polypeptide and spans a distance of approximately 150 kb. Previous studies have not precisely characterized the interval separating variable regions from joining--constant regions which is excised during gamma gene rearrangement. We report a series of overlapping cosmids which includes the portion of the gamma chain locus beginning with V2 and extends to the second exon of C2. Sixteen kilobases separate the most 3' variable region gene, V4, from the most 5' joining segment, J1.1.
Fox
V L
VL
Combined Program in Pediatric Gastroenterology and Nutrition, Children's Hospital, Boston, Massachusetts.
Strauss
W M
WM
Seidman
J G
JG
D003360:Cosmids; D015342:DNA Probes; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D006801:Humans; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D016692:Receptors, Antigen, T-Cell, gamma-delta; D015183:Restriction Mapping; D013601:T-Lymphocytes / Q000032:analysis
D015342:DNA Probes; D011948:Receptors, Antigen, T-Cell; D016692:Receptors, Antigen, T-Cell, gamma-delta