Cloning, expression, and nucleotide sequence of the Escherichia coli K-12 ackA gene.
The Escherichia coli K-12 ackA gene, which encodes an acetate kinase, was cloned. The acetate kinase activities of ackA+ plasmid-containing strains were amplified 160- to 180-fold. The complete nucleotide sequence of the ackA gene was determined. It was deduced that the ackA gene coded for a protein of 400 amino acids with an Mr of 43,297. The ackA gene was found to be located about 15 kilobases upstream of the purF-folC-hisT region of the chromosome.
Matsuyama
A
A
Research and Development Division, Kikkoman Corporation, Chiba-ken, Japan.
Yamamoto
H
H
Nakano
E
E
D000084:Acetate Kinase / Q000096:biosynthesis / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D003062:Codon / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005796:Genes; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010770:Phosphotransferases / Q000235:genetics*; D010957:Plasmids
D003062:Codon; D010770:Phosphotransferases; D000084:Acetate Kinase