title: Electron ionization mass spectrometry for sequence analysis of glycosphingolipid mixtures by fractional evaporation in the ion source. Mass spectrometric evidence for an eleven-sugar glycolipid.
abstract: This paper describes the use of electron ionization mass spectrometry for structural characterization of a mixture of glycosphingolipids from a single human kidney and presents mass spectrometric evidence for the presence of an eleven-sugar glycolipid. A large amount of structural information is obtained from minor glycolipid constituents having long sugar chains by a fractional evaporation in the ion source, achieved by steadily increasing the ion-source temperature.
authors.affiliation: Department of Medical Biochemistry, University of Gothenburg, Sweden.
authors.forename: J
authors.initials: J
authors.lastname: Holgersson
authors.forename: W
authors.initials: W
authors.lastname: Pimlott
authors.forename: B E
authors.initials: BE
authors.lastname: Samuelsson
authors.forename: M E
authors.initials: ME
authors.lastname: Breimer
mesh_terms: D002240:Carbohydrate Sequence; D006028:Glycosphingolipids / Q000737:chemistry*; D006801:Humans; D066298:In Vitro Techniques; D007668:Kidney / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data
chemical_list: D006028:Glycosphingolipids