Technical aspects of isolation extremity perfusion: experimental studies and clinical experience.
In experiments with dogs (n = 68), the influence of temperature, flow rate, perfusate, and perfusion duration on the hind leg subjected to an isolation perfusion was studied. The perfusion pressure, the blood gases, and the metabolic status in specimens of skeletal muscle obtained at the end of the perfusion period served as parameters. A perfusion of 1 and 2 h with whole blood, a flow rate of 10 mL/10 g/min, and temperatures of up to 42 degrees C did not result in alterations of the energy metabolism. When the flow rate was lowered to 5 mL/100 g/min, when other perfusates were used, or when the in temperature was raised to 43.5 degrees C, then tissue damage occurred. Knowledge gained in these experiments was utilized in the treatment of 371 patients with malignancies of the extremities, and an extremely low complication rate was observed.
Ghussen
F
F
Department of Surgery, University of Cologne, Federal Republic of Germany.
Krüger
I
I
D000818:Animals; D001132:Arm / Q000098:blood supply; D001783:Blood Flow Velocity; D002245:Carbon Dioxide / Q000097:blood; D010478:Chemotherapy, Cancer, Regional Perfusion / Q000009:adverse effects / Q000379:methods*; D004285:Dogs; D004317:Doxorubicin / Q000008:administration & dosage / Q000627:therapeutic use; D004734:Energy Metabolism; D005069:Evaluation Studies as Topic; D006614:Hindlimb / Q000098:blood supply* / Q000378:metabolism; D006801:Humans; D007866:Leg / Q000098:blood supply; D008545:Melanoma / Q000188:drug therapy; D008558:Melphalan / Q000008:administration & dosage / Q000627:therapeutic use; D009132:Muscles / Q000378:metabolism; D010100:Oxygen / Q000097:blood; D012509:Sarcoma / Q000188:drug therapy; D012983:Soft Tissue Neoplasms / Q000188:drug therapy; D013696:Temperature
D002245:Carbon Dioxide; D004317:Doxorubicin; D008558:Melphalan; D010100:Oxygen