Interleukin-2 induction of ACTH secretion: presence of an interleukin-2 receptor alpha-chain-like molecule on pituitary cells.
Pituitary cells were shown to release corticotropin (ACTH) in response to interleukin-2 (IL-2) and to express a protein that is related to the alpha-chain of the IL-2 receptor (IL-2R). The alpha-chain-like molecule was bound by a rat monoclonal antibody to the murine IL-2 receptor as well as to IL-2. Sodium dodecylsulfate-polyacrylamide gel electrophoretic analysis of the affinity-purified material from pituitary cells demonstrated a protein which was similar to that which was isolated from activated splenocytes. Thus, IL-2 and its receptor may be one of several hormone-receptor pairs utilized by both the immune and neuroendocrine systems for intersystem communication.
Smith
L R
LR
Department of Physiology and Biophysics, University of Alabama, Birmingham 35294.
Brown
S L
SL
Blalock
J E
JE
D000324:Adrenocorticotropic Hormone / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D055598:Chemical Phenomena; D002621:Chemistry; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006462:Hemolytic Plaque Technique; D007376:Interleukin-2 / Q000494:pharmacology*; D008970:Molecular Weight; D010902:Pituitary Gland / Q000378:metabolism*; D015375:Receptors, Interleukin-2 / Q000378:metabolism*; D013154:Spleen / Q000166:cytology / Q000378:metabolism
D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D000324:Adrenocorticotropic Hormone