Monoclonal antibodies to bovine major histocompatibility system antigens.
Of 89 monoclonal antibodies screened for anti-class I activity in a cytotoxic assay against bovine peripheral-blood lymphocytes, 6 reacted with all lymphocytes from all cattle tested, 72 failed to react at all and 11 reacted with polymorphic determinants. The reactivity of some of the 11 polymorphic monoclonal antibodies was dependent upon the bovine major histocompatibility system (BoLA) class I type. Eight monoclonal antibodies selected for putative anti-class II activity reacted with B-enriched lymphocytes from all cattle tested.
Mackie
J T
JT
Department of Immunology, John Curtin School of Medical Research, Australian National University, Canberra.
Bath
M L
ML
Stear
M J
MJ
Davis
W C
WC
D000818:Animals; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000276:immunology; D002417:Cattle; D003601:Cytotoxicity Tests, Immunologic; D015395:Histocompatibility Antigens Class I / Q000302:isolation & purification; D008970:Molecular Weight
D000911:Antibodies, Monoclonal; D015395:Histocompatibility Antigens Class I