Antigen-presenting cells for Lyt-2+ cells. II. Primary mixed-lymphocyte reactions stimulated by Ia+ dendritic cells and Ia- peritoneal exudate cells.
Information was sought on the antigen-presenting cells (APC) required for stimulating primary mixed-lymphocyte reactions (MLR) by unprimed Lyt-2+ cells in the absence of added lymphokines. Like L3T4+ cells, Lyt-2+ cells gave very high MLR in response to H-2-different dendritic cells (DC). Surprisingly, high MLR were also elicited by thioglycollate-induced peritoneal exudate cells (PEC), including Ia- PEC; these cells were non-immunogenic for L3T4+ cells. Since PEC consisted almost entirely of macrophages (M phi), the data suggest that at least two different cell types, DC and M phi, can express APC function for unprimed Lyt-2+ cells. Since resident peritoneal M phi and in vitro cultured PEC were poorly immunogenic, the APC function of M phi might be limited to a subset of these cells, e.g. to immature M phi.
Sprent
J
J
Department of Immunology, Research Institute of Scripps Clinic, La Jolla, CA 92037.
Schaefer
M
M
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D000950:Antigens, Ly; D003713:Dendritic Cells / Q000276:immunology; D000949:Histocompatibility Antigens Class II; D007959:Lymphocyte Culture Test, Mixed; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D010529:Peritoneal Cavity / Q000166:cytology; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D000950:Antigens, Ly; D000949:Histocompatibility Antigens Class II