title: Phenotypical heterogeneity among human T cell receptor gamma/delta-expressing clones derived from peripheral blood.
abstract: Human T cell clones expressing the T cell receptor (TcR) gamma/delta were isolated from peripheral blood lymphocytes of two unrelated donors. The TcR gamma/delta+ clones derived from one of these donors were all of the Ti gamma A+, delta-TCS1-, BB3+ phenotype indicating the exclusive use of the V gamma 9 and V delta 3 gene segments. In contrast, the T cell clones derived from the second donor were either Ti gamma A+, delta-TCS1-, BB3+:Ti gamma A-, delta-TCS1+, BB3- or Ti gamma A-, delta-TCS1-, BB3-. The delta-TCS1 determinant was expressed on both nondisulfide- and disulfide-linked TcR gamma/delta. Northern blot and DNA sequence analysis indicated that the Ti gamma A-, delta-TCS1-, BB3- clones do use the V delta 1 gene segment demonstrating that the delta-TCS1 monoclonal antibody does not react with all TcR gamma/delta using this particular gene segment. In contrast to the delta-TCS1+ T cell clones, the V delta 1+ delta-TCS1- T cell clones were found to express V delta 1 in conjunction with the J delta 3 gene segment suggesting that this particular V delta 1-J delta 3 combination is not recognized by the delta-TCS1 monoclonal antibody. In T cell clones derived from one individual the V delta 1 gene segment was found to be expressed with either J delta 1, J delta 2 or J delta 3. Heterogeneity among the 18 clones was detected with respect to the expression of the CD4, CD5 and CD8 antigens: one clone was CD4+, nine clones were CD5+ and two clones were CD8+. Thus, in this panel of clones, heterogeneity exists both with regard to CD antigen expression and the TcR gamma/delta phenotype. Also, our results indicate that the delta-TCS1 monoclonal antibody does not react with all TcR gamma/delta using the V delta 1 gene segment.
authors.affiliation: Department of Immunohematology and Blood Bank, University Hospital, Leiden, The Netherlands.
authors.forename: F
authors.initials: F
authors.lastname: Koning
authors.forename: M
authors.initials: M
authors.lastname: Knot
authors.forename: F
authors.initials: F
authors.lastname: Wassenaar
authors.forename: P
authors.initials: P
authors.lastname: Van den Elsen
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000032:analysis*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D001483:Base Sequence; D015152:Blotting, Northern; D017252:CD3 Complex; D002999:Clone Cells; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D016692:Receptors, Antigen, T-Cell, gamma-delta; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D011948:Receptors, Antigen, T-Cell; D016692:Receptors, Antigen, T-Cell, gamma-delta