[Neutralising properties for HIV virus of hybrid protein MalE-CD4 expressed in E. coli and purified in 1 step].
Genetic fusions allowing the expression in E. coli of hybrid proteins between a bacterial periplasmic maltose binding protein (MalE) and the CD4 molecule (the receptor of the HIV virus) have been constructed. One of them has kept most of the properties of each constituent: it is exported, can be purified in one step on an affinity column, interacts with anti-MalE and anti-CD4 antibodies, binds HIV gp 120 protein and inactivates HIV virus in an in vitro test.
Clément
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JM
Unité de Programmation moléculaire et Toxicologie génétique C.N.R.S.-U.A. n degrees 271, I.N.S.E.R.M. n degrees U 163.
Szmelcman
S
S
Jehanno
M
M
Martineau
P
P
Schwartz
O
O
Hofnung
M
M
D018528:ATP-Binding Cassette Transporters; D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins; D006678:HIV / Q000187:drug effects*; D015699:HIV Envelope Protein gp120; D007118:Immunoassay; D007163:Immunosorbent Techniques; D008320:Maltose / Q000378:metabolism; D058619:Maltose-Binding Proteins; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins; D033902:Periplasmic Binding Proteins; D011134:Polysaccharides / Q000378:metabolism; D015728:Receptors, HIV; D011991:Receptors, Virus / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology*; D012191:Retroviridae Proteins / Q000378:metabolism
D018528:ATP-Binding Cassette Transporters; D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D015699:HIV Envelope Protein gp120; C461619:MalE protein, E coli; D058619:Maltose-Binding Proteins; D009004:Monosaccharide Transport Proteins; D033902:Periplasmic Binding Proteins; D011134:Polysaccharides; D015728:Receptors, HIV; D011991:Receptors, Virus; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D012191:Retroviridae Proteins; C461599:maltose transport system, E coli; D008320:Maltose; C008315:maltodextrin
Propriétés neutralisantes pour le virus HIV d'une protéine hybride Ma1E-CD4 exprimée chez E. coli et purifiable en une étape.