Characterization of T-cell-receptor gamma (TRG) gene rearrangements in alloreactive T-cell clones.
Rearrangements of the T-cell Rearranging Gene (TRG) or T-cell-receptor gamma-chain genes were analyzed in 24 in vivo-sensitized alloreactive T-cell clones. This analysis represents the first complete assignment of TRG gene rearrangements to given variable and joining gene segments in nonleukemic T cells and provides some evidence for the hypothesis of sequential gamma genes rearrangements during T-lymphocyte differentiation. TRG gene rearrangements in our T-cell panel involved the known "active" V gamma genes, with a preferential use of V2 and V4 genes. In most clones, rearrangements occurred on both chromosomes and involved the J2 segment, but only 2 and 4 out of the 49 described rearrangements involved the additional J gamma segments JP1 and JP2, respectively. Two peculiar rearrangements were found. The first one was probably due to the creation of a new restriction enzyme site in the N-region at the V-J junction; the second can be explained by an aberrant rearrangement of a V gene to a sequence located between exons 2 and 3 of the TRGC1 gene.
Moisan
J P
JP
C.H.R. Nantes, Laboratoire de Biologie Moléculaire, France.
Bonneville
M
M
Bouyge
I
I
Moreau
J F
JF
Soulillou
J P
JP
Lefranc
M P
MP
D015139:Blotting, Southern; D002999:Clone Cells / Q000032:analysis; D015342:DNA Probes; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D006801:Humans; D015183:Restriction Mapping; D013601:T-Lymphocytes / Q000032:analysis*
D015342:DNA Probes