Autocrine induction of collagenase by serum amyloid A-like and beta 2-microglobulin-like proteins.
Two autocrine proteins of 14 and 12 kilodaltons that induce the synthesis of rabbit fibroblast collagenase were identified. The proteins were purified from serum-free culture medium taken from rabbit synovial fibroblasts stimulated with phorbol myristate acetate. The amino-terminal sequences of the 14- and 12-kilodalton species were approximately 60 to 80 percent homologous with serum amyloid A and beta 2 microglobulin, respectively. The polyacrylamide gel-eluted proteins retained the ability to induce collagenase synthesis in rabbit and human fibroblasts. These autocrine proteins may provide a means to modulate collagenase synthesis in normal remodeling as well as in inflammation and disease states.
Brinckerhoff
C E
CE
Department of Medicine, Dartmouth Medical School, Hanover, NH 03756.
Mitchell
T I
TI
Karmilowicz
M J
MJ
Kluve-Beckerman
B
B
Benson
M D
MD
D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D015342:DNA Probes; D004790:Enzyme Induction / Q000187:drug effects; D005347:Fibroblasts / Q000201:enzymology; D006801:Humans; D007163:Immunosorbent Techniques; D007525:Isoelectric Focusing; D003012:Microbial Collagenase / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger; D011817:Rabbits; D000685:Serum Amyloid A Protein / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D013583:Synovial Membrane / Q000201:enzymology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D001613:beta 2-Microglobulin / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*
D015342:DNA Probes; D012333:RNA, Messenger; D000685:Serum Amyloid A Protein; D001613:beta 2-Microglobulin; D003012:Microbial Collagenase; D013755:Tetradecanoylphorbol Acetate