Influence of sorbitol accumulation on growth and development of embryos cultured in elevated levels of glucose and fructose.
Day-9 rat embryos, cultured for 48 hours in 67 mmol/l D-glucose, showed impaired growth, increased malformation rate and elevated concentration of sorbitol compared to embryos cultured in medium without additional glucose supplement. Supplementing the high-glucose cultures with an aldose reductase inhibitor markedly decreased the sorbitol levels without affecting the malformations or the retarded growth of the embryos. Since embryos cultured in 6.6 mmol/l D-fructose showed normal growth and morphology despite increased accumulation of sorbitol, this study suggests a dissociation between raised sorbitol levels and glucose-induced maldevelopment in rat embryos.
Eriksson
U J
UJ
Department of Medical Cell Biology, University of Uppsala, Sweden.
Brolin
S E
SE
Naeser
P
P
D000014:Abnormalities, Drug-Induced; D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors; D000818:Animals; D004622:Embryo, Mammalian / Q000187:drug effects / Q000502:physiology*; D005260:Female; D005632:Fructose / Q000633:toxicity*; D005947:Glucose / Q000378:metabolism / Q000633:toxicity*; D009924:Organ Culture Techniques; D010793:Phthalazines / Q000494:pharmacology; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains; D013012:Sorbitol / Q000378:metabolism* / Q000494:pharmacology
D010793:Phthalazines; C042829:ponalrestat; D005632:Fructose; D013012:Sorbitol; D000449:Aldehyde Reductase; D005947:Glucose