An N-terminal transformation-governing sequence of SV40 large T antigen contributes to the binding of both p110Rb and a second cellular protein, p120.
In addition to Rb and p53, a third cellular protein, p120 in monkey and p118 in human cells, forms a specific complex with SV40 large T antigen (T). p118/120 is not a product of the Rb-gene. As was shown with T/Rb complex formation, the interaction between T and p120 is dependent on the intact nature of a ten residue, transformation-controlling domain in T (residues 105-114). In mouse cells, a readily detectable protein of 115 kd was detected, which, like murine Rb, also forms a stable complex with T. Like p118/120, p115 binding is also dependent on the intact nature of the 105-114 sequence. Given their similar size and T antigen binding sequence dependence, p115 and p118/120 may be products of the same gene in different species. These results suggest that interactions between T and p115/118/120, as well as T and Rb, contribute to the SV40 transforming mechanism.
Ewen
M E
ME
Dana-Farber Cancer Institute, Boston, Massachusetts 02115.
Ludlow
J W
JW
Marsilio
E
E
DeCaprio
J A
JA
Millikan
R C
RC
Cheng
S H
SH
Paucha
E
E
Livingston
D M
DM
D017456:Adenovirus Early Proteins; D000595:Amino Acid Sequence; D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000032:analysis* / Q000502:physiology; D000957:Antigens, Viral, Tumor / Q000378:metabolism; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000032:analysis* / Q000378:metabolism; D000882:Haplorhini; D006801:Humans; D051379:Mice; D009363:Neoplasm Proteins / Q000378:metabolism*; D009856:Oncogene Proteins, Viral / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D011233:Precipitin Tests; D016160:Retinoblastoma Protein; D016159:Tumor Suppressor Protein p53
D017456:Adenovirus Early Proteins; D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor; D009363:Neoplasm Proteins; D009856:Oncogene Proteins, Viral; D010750:Phosphoproteins; D016160:Retinoblastoma Protein; D016159:Tumor Suppressor Protein p53