Rp-diastereomer of adenosine cyclic 3',5'-phosphorothioate as an antagonist of the stimulatory action of cyclic AMP on the ouabain-insensitive Na efflux in single barnacle muscle fibers.
Single muscle fibers of the barnacle Balanus nubilus have been used as a preparation to test the possibility that the Rp-diastereomer of adenosine cyclic 3',5'-phosphorothioate, which is the first available analog of cAMP that acts as an antagonist of cAMP, may reduce the magnitude of cAMP-mediated stimulation of the resting ouabain-insensitive Na efflux. The results obtained show that this antagonist is, in fact, able to reduce stimulation of the Na efflux by injected cAMP in a dose-dependent manner.
Nwoga
J
J
Department of Physiology, University of Wisconsin, Madison 53706.
Bittar
E E
EE
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D000242:Cyclic AMP / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007700:Kinetics; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D010042:Ouabain / Q000494:pharmacology*; D012964:Sodium / Q000378:metabolism*; D013237:Stereoisomerism; D013873:Thionucleotides / Q000008:administration & dosage / Q000494:pharmacology*; D001468:Thoracica / Q000187:drug effects / Q000378:metabolism*
D013873:Thionucleotides; C016957:adenosine-3',5'-cyclic phosphorothioate; D010042:Ouabain; D012964:Sodium; D000242:Cyclic AMP