title: Adenovirus type 12 E1A down regulates expression of a transgene under control of a major histocompatibility complex class I promoter: evidence for transcriptional control.
abstract: The E1 region of human adenovirus type 12 (Ad12 E1) represses the expression of major histocompatibility complex (MHC) class I genes in transformed primary rodent cells. Conflicting results have been reported as to whether this E1A-mediated repression occurs at a transcriptional or a posttranscriptional level. In the present study, we show that in Ad12 E1-transformed primary baby mouse kidney cells from transgenic mice harboring the human growth hormone gene under control of an H-2K promoter both the expression of the endogenous MHC class I genes and the expression of the transgene are repressed. This experiment, as well as nuclear run-on analyses performed with single-stranded probes, revealed that Ad12 E1A inhibits MHC class I gene expression by repressing its promoter.
authors.affiliation: Laboratory for Molecular Carcinogenesis, Sylvius Laboratories, University of Leiden, The Netherlands.
authors.forename: I
authors.initials: I
authors.lastname: Meijer
authors.forename: A G
authors.initials: AG
authors.lastname: Jochemsen
authors.forename: C M
authors.initials: CM
authors.lastname: de Wit
authors.forename: J L
authors.initials: JL
authors.lastname: Bos
authors.forename: D
authors.initials: D
authors.lastname: Morello
authors.forename: A J
authors.initials: AJ
authors.lastname: van der Eb
mesh_terms: D017456:Adenovirus Early Proteins; D000818:Animals; D005786:Gene Expression Regulation / Q000187:drug effects; D005805:Genes, MHC Class I; D051379:Mice; D008810:Mice, Inbred C57BL; D009856:Oncogene Proteins, Viral / Q000494:pharmacology*; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic / Q000187:drug effects*
chemical_list: D017456:Adenovirus Early Proteins; D009856:Oncogene Proteins, Viral