title: Purification of a 51 kDa endo-beta-N-acetylglucosaminidase from Staphylococcus aureus.
abstract: A bacteriolytic enzyme obtained from the culture fluid of Staphylococcus aureus FDA 209P was purified to homogeneity utilizing dye-ligand affinity column chromatography, hydrophobic interaction high pressure liquid chromatography (HPLC) and hydroxyapatite HPLC. Subsequent characterizations indicated that the purified enzyme acted as endo-beta-N-acetylglucosaminidase. The molecular weight determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) was 51,000 and the isoelectric point was higher than 10. The optimum pH for the enzyme activity on whole cells of Micrococcus luteus as a substrate was 8.0. Some heavy metal cations (Cu2+ and Zn2+) inhibited the enzyme activity at a concentration of 0.1 mM and others (Ba2+, Mg2+ and Co2+) showed a stimulating effect at a concentration of 1 mM.
authors.affiliation: Department of Microbiology and Oral Bacteriology, Hiroshima University, School of Dentistry, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Sugai
authors.forename: H
authors.initials: H
authors.lastname: Koike
authors.forename: Y M
authors.initials: YM
authors.lastname: Hong
authors.forename: Y
authors.initials: Y
authors.lastname: Miyake
authors.forename: R
authors.initials: R
authors.lastname: Nogami
authors.forename: H
authors.initials: H
authors.lastname: Suginaka
mesh_terms: D000118:Acetylglucosaminidase / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006596:Hexosaminidases / Q000302:isolation & purification*; D007526:Isoelectric Point; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D008970:Molecular Weight; D013211:Staphylococcus aureus / Q000201:enzymology*
chemical_list: D006596:Hexosaminidases; D000118:Acetylglucosaminidase; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase