title: Transformation of Aspergillus flavus to study aflatoxin biosynthesis.
abstract: Aflatoxin contamination of agricultural commodities continues to be a serious problem in the United States. Breeding for resistant genotypes has been unsuccessful and detoxification of food sources is not economically feasible. New strategies for control may become apparent once more is known about the biosynthesis and regulation of aflatoxin. Although the biosynthetic pathway of aflatoxin has been extensively studied, little is known about the regulation of the individual steps in the pathway. We have developed a genetic transformation system for Aspergillus flavus that provides a new and expedient approach to studying the biosynthesis of aflatoxin and its regulation. Through the use of this genetic transformation system, genes for aflatoxin biosynthesis can be identified and isolated by the complementation of aflatoxin negative mutants. In this paper we discuss molecular strategies for studying the regulation and biosynthesis of aflatoxin.
authors.affiliation: Department of Plant Pathology, North Carolina State University, Raleigh 27695-7616.
authors.forename: G A
authors.initials: GA
authors.lastname: Payne
authors.forename: C P
authors.initials: CP
authors.lastname: Woloshuk
mesh_terms: D000348:Aflatoxins / Q000096:biosynthesis* / Q000235:genetics; D001231:Aspergillus flavus / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005819:Genetic Markers; D015698:Genomic Library; D014170:Transformation, Genetic
chemical_list: D000348:Aflatoxins; D005819:Genetic Markers