Integration host factor (IHF) stimulates binding of the gpNu1 subunit of lambda terminase to cos DNA.
The lambda terminase enzyme binds to the cohesive end sites (cos) of multimeric replicating lambda DNA and introduces staggered nicks to regenerate the 12 bp single-stranded cohesive ends of the mature phage genome. In vitro this endonucleolytic cleavage requires spermidine, magnesium ions, ATP and a host factor. One of the E. coli proteins which can fulfill this latter requirement is Integration Host Factor (IHF). IHF and the gpNu1 subunit of terminase can bind simultaneously to their own specific binding sites at cos. DNase I footprinting experiments suggest that IHF may promote gpNu1 binding. Although no specific gpNu1 binding to the left side of cos can be detected, this DNA segment does play a specific role since a cos fragment that does not include the left side or whose left side is replaced by non-cos sequences, is unable to bind gpNu1 unless either spermidine or IHF is present. Binding studies on the right side of cos using individual or combinations of gpNu1 binding sites I, II and III indicate that binding at sites I and II is not optimal unless site III is present.
Shinder
G
G
Department of Medical Genetics, University of Toronto, Ontario, Canada.
Gold
M
M
D001287:Attachment Sites, Microbiological / Q000187:drug effects; D001426:Bacterial Proteins / Q000502:physiology*; D010582:Bacteriophage lambda / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D003850:Deoxyribonuclease I; D004706:Endodeoxyribonucleases / Q000235:genetics / Q000378:metabolism*; D034982:Integration Host Factors; D008242:Lysogeny / Q000187:drug effects; D009690:Nucleic Acid Conformation / Q000187:drug effects; D013095:Spermidine
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D034982:Integration Host Factors; D004706:Endodeoxyribonucleases; C030894:terminase; D003850:Deoxyribonuclease I; D013095:Spermidine