[Ammonium molybdate as stabilizing agent in tetramethyl benzidine reaction of CB-HRP neurohistochemistry and its application to electron microscopy and immunocytochemistry].
Ammonium molybdate (AHM) was used as a stabilizing agent in the tetramethyl benzidine (TMB) reaction of choleragen subunit B conjugated horseradish peroxidase (CB-HRP) neurohistochemistry. In comparison with Mesulam's TMB method using sodium nitroprusside (SNP) as stabilizing agent, this new stabilizer offers similar sensitivity in regard to visualization of CB-HRP labelled neurons and their extranuclear Golgi-phobic dendrites. The nearly physiological pH (6-8) of the reaction medium demonstrates better preservation of tissue and cell structures of reacted sections and avoids non-specific needle-like crystal formation. Under the electron microscope, the histochemical reaction products can be clearly distinguished, without the usual shrinkage or distention of cellular and subcellular structures. This method was also applied with success to immunocytochemistry through small modifications.
Liang
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Wan
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D000818:Animals; D001560:Benzidines; D003712:Dendrites / Q000378:metabolism / Q000648:ultrastructure*; D006735:Horseradish Peroxidase; D007150:Immunohistochemistry; D008982:Molybdenum; D009474:Neurons / Q000378:metabolism / Q000648:ultrastructure*; D051381:Rats
D001560:Benzidines; C021758:3,3',5,5'-tetramethylbenzidine; D008982:Molybdenum; D006735:Horseradish Peroxidase; C022175:ammonium molybdate