title: The anti-Leu4 (CD3) monoclonal antibody reacts with proximal tubular cells of the human kidney.
abstract: A recent observation that certain kidney tubular cells, especially those in the normal kidney, were positively stained with the anti-Leu4 monoclonal antibody (MoAb) led us to investigate systematically the reactivity of three different anti-CD3 MoAb with a panel of normal human kidneys. Using immunohistochemical and immunofluorescence staining techniques, the anti-Leu4 MoAb was found to react with proximal tubular cells in all eight examined kidneys, while no tubular-cell reactivity was observed with the OKT3 or DAKO-T3 MoAb. In each kidney, however, all three antibodies reacted with a similar number of occasionally encountered T cells. The G11 MoAb, which has been reported to react with a calcium receptor-associated molecule, and the anti-Leu4 MoAb showed almost identical patterns of tubular-cell reactivity. The observed co-distribution of Leu4-expressing structures with a putative Ca2+ sensor in the kidney and the fact that binding of anti-Leu4 to T cells induces Ca2+-mediated signal transduction, warrant further studies on a potential role of Leu4-expressing structures in Ca2+ regulation of proximal tubular cells.
authors.affiliation: Department of Clinical Bacteriology, University of Uppsala, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Karlsson-Parra
authors.forename: E
authors.initials: E
authors.lastname: Dimény
authors.forename: C
authors.initials: C
authors.lastname: Juhlin
authors.forename: B
authors.initials: B
authors.lastname: Fellström
authors.forename: L
authors.initials: L
authors.lastname: Klareskog
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis* / Q000276:immunology; D017252:CD3 Complex; D006801:Humans; D007150:Immunohistochemistry; D007687:Kidney Tubules, Proximal / Q000166:cytology / Q000276:immunology*; D016853:Muromonab-CD3; D009393:Nephritis / Q000276:immunology; D010280:Parathyroid Glands / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000032:analysis* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D016853:Muromonab-CD3; D011948:Receptors, Antigen, T-Cell