Human cell lines stably expressing HIV env and tat gene products.
A DNA fragment containing the tat, rev and env genes of the human immunodeficiency virus type 1 was inserted into the retroviral vector pZIPneoAU3. The resulting plasmid penvAU3 was transfected into HeLa and psi CRIP cells. Resulting recombinant retroviruses were used to infect HeLa and Jurkat cells. Immunoprecipitation analysis of stable transformants showed the expression of HIV env glycoproteins gp160, gp120 and gp41. Transactivation assays with a plasmid containing the gene for chloramphenicol acetyltransferase linked to HIV promoter-enhancer sequences demonstrated the expression of functional tat. These cells constitute virus-free tools for functional and structural studies of native env and tat.
Sosa
M A
MA
Dana-Farber Cancer Institute, Boston, MA.
DeGasperi
R
R
Fazely
F
F
Ruprecht
R M
RM
D015500:Chloramphenicol O-Acetyltransferase / Q000378:metabolism; D005786:Gene Expression Regulation; D015696:Gene Products, tat; D005814:Genes, Viral; D005822:Genetic Vectors; D006678:HIV / Q000235:genetics*; D006367:HeLa Cells / Q000032:analysis* / Q000201:enzymology / Q000382:microbiology; D006801:Humans; D012191:Retroviridae Proteins / Q000235:genetics* / Q000302:isolation & purification; D014157:Transcription Factors / Q000235:genetics* / Q000302:isolation & purification; D014162:Transfection; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000302:isolation & purification; D054322:tat Gene Products, Human Immunodeficiency Virus
D015696:Gene Products, tat; D012191:Retroviridae Proteins; D014157:Transcription Factors; D014759:Viral Envelope Proteins; D054322:tat Gene Products, Human Immunodeficiency Virus; D015500:Chloramphenicol O-Acetyltransferase