title: Eicosanoids in acute and chronic psoriatic lesions: leukotriene B4, but not 12-hydroxy-eicosatetraenoic acid, is present in biologically active amounts in acute guttate lesions.
abstract: The biochemical changes underlying the clinical manifestations of psoriasis are unknown. Certain chemotactic eicosanoids derived from arachidonic acid metabolism have been suggested to play important roles in psoriasis, because of their presence in lesional psoriatic skin and their ability to elicit skin inflammation and to stimulate epidermal proliferation. The purpose of the present study was to elucidate which eicosanoids might be involved in the early phases of the inflammatory processes of psoriasis. Eicosanoids were analyzed in scale and in lesional skin without scale both in acute guttate and chronic plaque psoriatic lesions. Methods for identification of eicosanoids included reversed-phase high-performance liquid chromatography combined with radioimmunoassay. Leukotriene B4 was present in both acute guttate and chronic plaque skin lesions in biologically active amounts (acute guttate lesions: 18.7 +/- 7.1 ng/g wet tissue in scale and 3.2 +/- 1.5 ng/g wet tissue in lesional skin without scale; chronic plaque lesions: 33.1 +/- 9.7 ng/g wet tissue in scale and 5.3 +/- 2.0 ng/g wet tissue in lesional skin without scale). 12- and 15-hydroxy-eicosatetraenoic acid (HETE) reached biologically active concentrations only in scale of chronic plaque lesions (1,512 +/- 282 and 1,441 +/- 411 ng/g wet tissue, respectively). The level of prostaglandin E2 in chronic plaque lesions was similar to the level in normal skin, while the level in acute guttate lesions was increased twofold (71.0 +/- 14.8 ng/g wet tissue). These results demonstrate that leukotriene B4, but not 12-HETE, is present in acute guttate psoriatic skin lesions in concentrations able to exert biologic effects. Leukotriene B4 may therefore participate in inflammatory changes of acute psoriasis.
authors.affiliation: Department of Dermatology, Marselisborg Hospital, University of Aarhus, Denmark.
authors.forename: K
authors.initials: K
authors.lastname: Fogh
authors.forename: T
authors.initials: T
authors.lastname: Herlin
authors.forename: K
authors.initials: K
authors.lastname: Kragballe
mesh_terms: D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D000208:Acute Disease; D002851:Chromatography, High Pressure Liquid; D002908:Chronic Disease; D015232:Dinoprostone / Q000032:analysis*; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000032:analysis*; D007975:Leukotriene B4 / Q000032:analysis*; D011565:Psoriasis / Q000378:metabolism*; D011863:Radioimmunoassay; D012867:Skin / Q000032:analysis*
chemical_list: D006893:Hydroxyeicosatetraenoic Acids; D007975:Leukotriene B4; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid; D015232:Dinoprostone