[Effects of lipoxin B on colony-forming capacity of human peripheral blood mononuclear cells in diffusion chambers].
The lipoxin B (5, 14, 15-trihydroxy-6, 8, 10, 12-eicosatetraenoate) obtained by the enzymatic peroxidation of 5, 15-dihydroxy-6, 8, 11, 13-eicosatetraenoate (5, 15-DHETE) with the homogenous rabbit reticulocytes lipoxygenase and purified by HPLC stimulates the proliferation and differentiation of granulocyte-monocyte colony formation units (CFU-GMdc) of human peripheral blood at 3.6 X 10(-9) 3.6 X 10(-8)M in diffusion chamber placed in mouse abdominal cavity. The lipoxin B precursors namely 5,15-DHETE and arachidonic acid had stimulating influence at about 10(-6) and 10(-3)M respectively. The formation of polynuclear cells under the action of the studied lipoxygenase metabolites of the arachidonic acid was shown.
Popov
G K
GK
Nekrasov
A S
AS
Khshivo
A L
AL
Pochinskiĭ
A G
AG
Lankin
V Z
VZ
D000328:Adult; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism / Q000494:pharmacology; D003114:Colony-Forming Units Assay / Q000379:methods*; D015118:Eicosapentaenoic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D006098:Granulocytes / Q000187:drug effects*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects*; D044045:Lipoxins; D008084:Lipoxygenase / Q000378:metabolism; D008297:Male; D051379:Mice; D011817:Rabbits; D012156:Reticulocytes / Q000201:enzymology; D013234:Stem Cells / Q000187:drug effects*
D001095:Arachidonic Acids; D044045:Lipoxins; D016718:Arachidonic Acid; C044355:lipoxin B5; D015118:Eicosapentaenoic Acid; D008084:Lipoxygenase
Vliianie lipoksina B na kolonieobrazuiushchuiu sposobnost' mononuklearov perifericheskoĭ krovi cheloveka v usloviiakh diffuzionnoĭ kamery.