Production and characterization of a rat monoclonal antibody against the murine CD3 molecular complex.
We describe the production of a rat monoclonal antibody, 17A2, that detects the T cell receptor-associated CD3 molecular complex. 17A2 cross-competes with a CD3 epsilon-specific reagent and similarly stimulates IL-2 production by T cells. Immunohistological and cell separation applications are shown.
Miescher
G C
GC
Ludwig Institute for Cancer Research, Lausanne Branch, Epalinges, Switzerland.
Schreyer
M
M
MacDonald
H R
HR
D000818:Animals; D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D017252:CD3 Complex; D005260:Female; D007150:Immunohistochemistry; D007376:Interleukin-2 / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D051381:Rats; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D007376:Interleukin-2; D011948:Receptors, Antigen, T-Cell