Effect of rat cardionatrin I (rat ANF 99-126) on the response of toad skin to angiotensin II.
The atrial natriuretic peptide cardionatrin I (cardionatrin I is ANF 99-126) was used in studies directed to assess its effects on osmotic water permeability (Posm) and short-circuit current (SCC) in isolated toad skin. Results showed that ANF 99-126 (10(-7) M) added to the dermal side of the skin had no effect on basal Posm or SCC. However, ANF 99-126 (3.3 x 10(-8) M) was able to produce a 50% reversible inhibition of the maximal Posm response to angiotensin II (AII) (3.2 x 10(-8) M). These effects were seen when the skins were preincubated with ANF 99-126 for 10 min or less before the addition of AII. Longer preincubation appeared to inactivate ANF 99-126 through proteolysis. ANF 99-126(10(-7) M) failed to inhibit the SCC response to AII (10(-5) M) in toad skin. These results are compatible with a modulatory function for ANF on several systems including those involved in the regulation of extracellular fluid volume.
Coviello
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Department of Physiology, INSIBIO, School of Medicine, Universidad Nacional de Tucuman, Argentina.
Soria
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Proto
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Berman
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Gamundi
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Alonso
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de Bold
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AJ
D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D002022:Bufo arenarum; D004232:Diuretics / Q000494:pharmacology; D004848:Epithelium / Q000187:drug effects / Q000502:physiology; D005260:Female; D005399:Fishes; D066298:In Vitro Techniques; D008297:Male; D010446:Peptide Fragments / Q000494:pharmacology*; D010539:Permeability; D051381:Rats; D012867:Skin / Q000187:drug effects; D012879:Skin Physiological Phenomena
D004232:Diuretics; D010446:Peptide Fragments; D000804:Angiotensin II; D009320:Atrial Natriuretic Factor