[The use of the multicopy plasmid pUC19 for assuring the constitutive expression of gene rplL in Escherichia coli].
A mutation in lac-operator region of pUC19 plasmid causing an increase in beta-galactosidase activity was observed. The plasmid was used as a vector to provide high level of expression of the cloned E. coli rplL gene.
Zolotukhin
S B
SB
Zhivolup
A N
AN
Krupskaia
I V
IV
Budmaska
M I
MI
Paton
E B
EB
D003001:Cloning, Molecular / Q000379:methods; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005822:Genetic Vectors; D007763:Lac Operon; D009154:Mutation; D010641:Phenotype; D010957:Plasmids / Q000235:genetics*; D001616:beta-Galactosidase / Q000235:genetics
D004269:DNA, Bacterial; D001616:beta-Galactosidase
Ispol'zovanie mnogokopiĭnoĭ plazmidy pUC19 dlia obespecheniia konstitutivnoĭ ékspressii gena rplL Escherichia coli.