A rat mutant cell clone showing temperature-dependent transformed phenotypes with functional expression of the src gene product.
The cellular mutant B814 isolated from a Fischer rat cell line shows temperature-sensitivity of focus formation on infection with Moloney murine sarcoma virus (Mo-MSV) and Rous sarcoma virus (RSV). An RSV-transformed clone (S814-2) isolated from B814 cells shows temperature-sensitive transformed phenotypes for morphology, growth in soft agar, and glucose uptake. The expression, phosphorylation, and tyrosine kinase activity of pp60v-src in S814-2 were not affected at the nonpermissive temperature, and virus rescued from this clone had wild-type transforming ability, suggesting that a cellular factor altered in S814-2 is responsible for the cellular steps of transformation after the function of pp60v-src. In addition, the cellular 36K protein, a possible candidate as a target of pp60v-src, was phosphorylated at the nonpermissive temperature in S814-2, indicating that phosphorylation of the 36K protein is not correlated with transformed phenotypes.
Inoue
H
H
Department of Tumor Virology, Osaka University, Japan.
Owada
M K
MK
Yutsudo
M
M
Hakura
A
A
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000502:physiology; D002460:Cell Line; D002472:Cell Transformation, Viral; D002642:Chick Embryo; D005347:Fibroblasts; D005786:Gene Expression Regulation; D007708:Kirsten murine sarcoma virus / Q000502:physiology; D008980:Moloney murine sarcoma virus / Q000502:physiology; D009154:Mutation; D015688:Oncogene Protein pp60(v-src); D010641:Phenotype; D010766:Phosphorylation; D011233:Precipitin Tests; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D051381:Rats; D012190:Retroviridae / Q000502:physiology*; D012191:Retroviridae Proteins / Q000235:genetics* / Q000378:metabolism; D013696:Temperature
D011518:Proto-Oncogene Proteins; D012191:Retroviridae Proteins; D015688:Oncogene Protein pp60(v-src)