Altered formation of leukotriene B4 in vitro by synovial fluid neutrophils in rheumatoid arthritis.
The synthesis of products of the 5-lipoxygenase pathway by synovial fluid (SF) and peripheral blood neutrophils of 12 patients with rheumatoid arthritis was compared in 3 experimental conditions using high performance liquid chromatographic analysis. Major differences were observed when blood and SF neutrophils were incubated with ionophore A23187, the formation of all 5-lipoxygenase products being lower (p less than 0.0005) in the SF neutrophils. Addition of exogenous arachidonic acid to the A23187 stimulated cells partially overcame the difference in 5-lipoxygenase product synthesis between the 2 neutrophil populations. In contrast, upon incubation with arachidonic acid alone, SF neutrophils produced significantly larger amounts of 5-lipoxygenase products. The increased reactivity of the SF neutrophil 5-lipoxygenase to arachidonic acid and the decreased 5-lipoxygenase product synthesis upon A23187 stimulation may be the consequence of the previous activation of the cells and 5-lipoxygenase product synthesis in situ.
Poubelle
P E
PE
Department of Medicine, Centre Hospitalier de l'Université Laval, Québec, Canada.
Bourgoin
S
S
McColl
S R
SR
Nguyen
M H
MH
Beaulieu
A D
AD
Borgeat
P
P
D000328:Adult; D000368:Aged; D001095:Arachidonic Acids / Q000494:pharmacology; D001172:Arthritis, Rheumatoid / Q000378:metabolism*; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D007832:Lasalocid / Q000494:pharmacology; D017572:Leukotriene A4; D007975:Leukotriene B4 / Q000096:biosynthesis*; D015289:Leukotrienes / Q000096:biosynthesis; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D013189:SRS-A / Q000096:biosynthesis; D013582:Synovial Fluid / Q000187:drug effects / Q000378:metabolism*
D001095:Arachidonic Acids; D017572:Leukotriene A4; D015289:Leukotrienes; D013189:SRS-A; D007975:Leukotriene B4; D007832:Lasalocid