title: Control of gene expression by artificial introns in Saccharomyces cerevisiae.
abstract: Artificial yeast introns that show cold-sensitive splicing have been constructed. These conditional introns can be inserted into a target gene as an "intron cassette" without disrupting the coding information, allowing expression of the gene to be cold sensitive. Insertion of these intron cassettes rendered the yeast URA3 gene cold sensitive in its expression. The advantage of this intron-mediated control system is that any gene can be converted to a controllable gene by simple insertion of an intron.
authors.affiliation: Institute for Biotechnology Research, Wakunaga Pharmaceutical Co., Ltd., Hiroshima, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yoshimatsu
authors.forename: F
authors.initials: F
authors.lastname: Nagawa
mesh_terms: D001483:Base Sequence; D003080:Cold Temperature; D004251:DNA Transposable Elements; D005786:Gene Expression Regulation; D005818:Genetic Engineering; D007438:Introns; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements