title: The role of suppressor factors in the regulation of immune responses by ultraviolet radiation-induced suppressor T lymphocytes. II. Activity of suppressor cell culture sonicates.
abstract: The purpose of this study was to determine whether multiple types of suppressor factors play a role in the regulation of immune responses by ultraviolet radiation-induced suppressor T lymphocytes (UV Ts). The UV Ts were induced by applying contact allergens to the ventral, unirradiated skin of mice exposed 5 days earlier to UVB radiation. Previous studies indicated that supernatants from cultures containing UV Ts, normal lymphocytes, and hapten-modified cells suppressed contact hypersensitivity (CHS) in vivo and cytotoxic T lymphocyte (CTL) generation in vitro in a hapten-specific manner. In this report, cell-free lysates from sonically disrupted UV Ts were examined for their ability to suppress these responses. When lysates were injected into normal animals at the time of sensitization, they inhibited CHS in a hapten-nonspecific manner. In addition, the lysates suppressed not only the induction but also the elicitation of CHS, and they suppressed the generation of CTL. Lysates prepared from spleen cells obtained from non-UV-irradiated mice or UV-irradiated, unsensitized mice failed to inhibit either response. Moreover, in contrast to the lysates, the hapten-specific UV Ts culture supernatants inhibited the induction but not the elicitation of CHS. These results suggest that both hapten-specific and nonspecific inhibitory factors may participate in the regulation of immune responses by UV Ts.
authors.affiliation: Department of Immunology, University of Texas M.D. Anderson Cancer Center, Houston.
authors.forename: G K
authors.initials: GK
authors.lastname: Yee
authors.forename: S E
authors.initials: SE
authors.lastname: Ullrich
authors.forename: M L
authors.initials: ML
authors.lastname: Kripke
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003877:Dermatitis, Contact / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D013010:Sonication; D013491:Suppressor Factors, Immunologic / Q000096:biosynthesis / Q000502:physiology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D013491:Suppressor Factors, Immunologic