Chicken anemia agent in the United States: isolation of the virus and detection of antibody in broiler breeder flocks.
Chicken anemia agent (CAA) was isolated from broiler chickens in Texas with a blue wing or anemia dermatitis-like syndrome. Specific-pathogen-free chicks inoculated with field material developed anemia, and CAA was isolated in MDCC-MSB1 cells from bone marrow and lymphoid tissue from inoculated chicks. One isolate, designated EF88/78/276, was further characterized. Infectivity of EF88/78/276 was resistant to treatment with chloroform and with heat at 70 C for 5 minutes. EF88/78/276 was indistinguishable from the Cux-1 and Gifu-1 isolates of CAA by cross-neutralization tests. Almost all 1-day-old susceptible chicks inoculated intramuscularly with EF88/78/276 developed anemia, but contact-infected chicks did not. Antibody to CAA was detected in broiler breeder flocks from Texas, the Delmarva peninsula, and Alabama.
McNulty
M S
MS
Veterinary Research Laboratories, Stormont, Belfast, Northern, Ireland.
Connor
T J
TJ
McNeilly
F
F
Spackman
D
D
D000740:Anemia / Q000209:etiology / Q000382:microbiology / Q000662:veterinary*; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002645:Chickens / Q000276:immunology / Q000382:microbiology*; D007106:Immune Sera / Q000276:immunology; D011201:Poultry Diseases / Q000209:etiology / Q000276:immunology / Q000382:microbiology*; D012698:Serologic Tests; D013047:Specific Pathogen-Free Organisms; D014777:Virus Diseases / Q000209:etiology / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*
D000914:Antibodies, Viral; D007106:Immune Sera