Juvenile and adult laryngeal papillomata: classification by in-situ hybridization for human papillomavirus.
We report the application of an in-situ hybridization technique for the demonstration of human papillomavirus (HPV) employing a biotin-streptavidin-polyalkaline phosphatase complex to paraffin processed tissue from a series of patients with laryngeal papillomata. All cases of juvenile papillomata, whether solitary or multiple, proved positive for HPV types 6 and/or type 11. However, only two cases of adult solitary papillomata and five cases of adult multiple papillomata were positive for HPV type 6 and/or type 11. All papillomata were negative for HPV types 16 and 18. Five specimens of normal vocal cord epithelium were uniformly negative for all four HPV types.
Terry
R M
RM
Department of ENT Surgery, Leeds General Infirmary, UK.
Lewis
F A
FA
Robertson
S
S
Blythe
D
D
Wells
M
M
D000293:Adolescent; D000328:Adult; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000382:microbiology*; D009378:Neoplasms, Multiple Primary / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D010212:Papilloma / Q000382:microbiology*; D027383:Papillomaviridae / Q000302:isolation & purification*
D015344:DNA Probes, HPV; D004279:DNA, Viral