Nucleotide sequence of the gene for a fibronectin-binding protein from Staphylococcus aureus: use of this peptide sequence in the synthesis of biologically active peptides.
Binding of cells of Staphylococcus aureus to fibronectin, which may represent a mechanism of host tissue adherence, involves a fibronectin-receptor protein present on the bacterial surface. Cloning of a gene coding for a staphylococcal fibronectin-binding protein and construction of a fusion protein with fibronectin-binding properties was previously reported from our laboratory. We have now sequenced the gene and deduced a primary sequence of the fibronectin-binding protein. The protein resembles other cell-wall-associated proteins on Gram-positive bacteria in that it (i) appears to be anchored in the cell membrane via its C-terminal end, (ii) contains a proline-rich repeating unit outside the membrane anchor, and (iii) contains a long (36-amino acid) signal sequence at the N terminus. The fibronectin-binding activity has been localized to a domain composed of a 38-amino acid unit repeated completely three times and partially a fourth time; the identity between the three 38-amino acid sequences varies from 42 to 87%. Three synthetic peptides mimicking the structure of each 38-amino acid unit were constructed. All three peptides interacted with fibronectin, as indicated by their ability to inhibit binding of fibronectin to staphylococcal cells, whereas an unrelated 37-amino acid peptide showed no inhibitory activity.
Signäs
C
C
Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala.
Raucci
G
G
Jönsson
K
K
Lindgren
P E
PE
Anantharamaiah
G M
GM
Höök
M
M
Lindberg
M
M
D000595:Amino Acid Sequence; D001422:Bacterial Adhesion; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics*; D005353:Fibronectins / Q000378:metabolism*; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic / Q000096:biosynthesis / Q000235:genetics*; D013211:Staphylococcus aureus / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
D004269:DNA, Bacterial; D005353:Fibronectins; D010455:Peptides; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic