Determination of phosphonoformate (foscarnet) in biological fluids by ion-pair reversed-phase liquid chromatography.
Bioanalytical liquid chromatographic methods for the determination of phosphonoformate (foscarnet) have been developed. Biological fluids, after simple pre-treatment (ultrafiltration and/or treatment with charcoal), were injected into a reversed-phase liquid chromatographic system with electrochemical detection. Foscarnet was retained as an ion pair with tetrahexylammonium; addition of pyrophosphate was necessary in order to obtain an acceptable peak. This additive could also be used for the fine regulation of the retention to achieve the necessary selectivity.
Pettersson
K J
KJ
Department of Bioanalysis, Astra Research Centre, Södertälje, Sweden.
Nordgren
T
T
Westerlund
D
D
D002851:Chromatography, High Pressure Liquid; D017245:Foscarnet; D006801:Humans; D009943:Organophosphorus Compounds / Q000097:blood*; D010746:Phosphonoacetic Acid / Q000031:analogs & derivatives / Q000097:blood* / Q000134:cerebrospinal fluid / Q000652:urine; D013056:Spectrophotometry, Ultraviolet
D009943:Organophosphorus Compounds; D017245:Foscarnet; D010746:Phosphonoacetic Acid