Transformation of NIH 3T3 cells by herpes simplex type 2 BglII n fragment and sub-fragments is independent from induction of mutation at the HPRT locus.
The effect of transfection of the herpes simplex virus type 2 transforming fragment BglII n and of its three Xhol subfragments on mutagenesis and morphological transformation was assayed in NIH 3T3 cells. While BglII n and the right hand portion of this fragment increased the number of transformed foci, no significant effects on the mutation frequency at the hprt locus were observed. Our results indicate that transformation by BglII n is independent from the induction of somatic mutations and suggest that other mechanisms must be considered to explain transformation by this sequence.
Pilon
L
L
Centre Hospitalier de l'Université Laval, Québec, Canada.
Kessous-Elbaz
A
A
Langelier
Y
Y
Royal
A
A
D000818:Animals; D001426:Bacterial Proteins; D002470:Cell Survival; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D004279:DNA, Viral / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D051379:Mice; D009154:Mutation; D010957:Plasmids; D018139:Simplexvirus / Q000235:genetics*; D014162:Transfection
D001426:Bacterial Proteins; D004279:DNA, Viral; D007041:Hypoxanthine Phosphoribosyltransferase; C024404:BglII endonuclease; D015252:Deoxyribonucleases, Type II Site-Specific