Bialaphos resistance as a dominant selectable marker in Neurospora crassa.
Conidia of Neurospora crassa are sensitive to the herbicide bialaphos at concentrations of 160 mg/l in Westergaard's or Fries' minimal media. Plasmid pJA4 was constructed by inserting a truncated bar gene from Streptomyces hygroscopicus fused to the his-3 promoter from N. crassa into pUC19. The bar gene in plasmid pJA4 confers resistance to bialaphos when transformants are selected on a medium containing bialaphos. The bar gene can be used as an additional dominant selectable marker for transformation of fungi.
Avalos
J
J
Department of Genetics, University of Georgia, Athens 30602.
Geever
R F
RF
Case
M E
ME
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015139:Blotting, Southern; D004352:Drug Resistance, Microbial / Q000235:genetics; D005798:Genes, Bacterial; D005800:Genes, Fungal; D005819:Genetic Markers; D009491:Neurospora / Q000235:genetics*; D009492:Neurospora crassa / Q000187:drug effects / Q000235:genetics*; D009943:Organophosphorus Compounds / Q000494:pharmacology*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D013302:Streptomyces / Q000235:genetics; D014170:Transformation, Genetic
D000900:Anti-Bacterial Agents; D005819:Genetic Markers; D009943:Organophosphorus Compounds; C003122:bialaphos