Interleukin-2 stimulates osteoclastic activity: increased acid production and radioactive calcium release.
Recombinant human interleukin-2 (IL-2) was studied to determine effects on acid production by individual osteoclasts in situ on mouse calvarial bones. This analysis was performed using a microspectrofluorimetric technique to quantify acid production in individual cells. Radioactive calcium release was determined using calvarial bones in a standard tissue culture system. This allowed us to correlate changes in acid production with a measure of bone resorption. IL-2 stimulated acid production and bone resorbing activity. Both effects were inhibited by calcitonin. No stimulation of bone resorption occurred when IL-2-containing test media was incubated with a specific anti-IL-2 antibody and ultrafiltered. Our data demonstrated a correlation between acid production and bone resorbing activity in mouse calvaria exposed to parathyroid hormone (PTH). The data obtained from cultured mouse calvaria exposed to IL-2 demonstrated similar stimulatory effects to those seen during PTH exposure. These data suggest that calvaria exposed to IL-2 in vitro have increased osteoclastic acid production corresponding with increased bone resorption.
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D000143:Acids / Q000378:metabolism; D000165:Acridine Orange; D000818:Animals; D001862:Bone Resorption / Q000378:metabolism* / Q000503:physiopathology; D001842:Bone and Bones / Q000378:metabolism*; D002116:Calcitonin / Q000494:pharmacology; D002118:Calcium / Q000493:pharmacokinetics*; D002132:Calcium Radioisotopes; D046508:Culture Techniques; D007376:Interleukin-2 / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010010:Osteoclasts / Q000187:drug effects* / Q000378:metabolism; D010281:Parathyroid Hormone / Q000037:antagonists & inhibitors / Q000494:pharmacology; D011994:Recombinant Proteins
D000143:Acids; D002132:Calcium Radioisotopes; D007376:Interleukin-2; D010281:Parathyroid Hormone; D011994:Recombinant Proteins; D002116:Calcitonin; D000165:Acridine Orange; D002118:Calcium