Quantitation of autoantibody-secreting B cells in systemic lupus erythematosus.
An ELISA spot assay was used to quantitate the number of autoantibody-secreting B cells in the peripheral blood of patients with systemic lupus erythematosus. Patients with active disease had 20 fold more anti-DNA, 4 fold more anti-actin and 3 fold more anti-myosin secreting lymphocytes than controls but normal numbers of anti-cardiolipin and anti-transferrin secreting B cells. 60% of SLE patients had increased numbers of B cells reactive with multiple autoantigens. These data suggest that B cell activation in SLE may be influenced by both antigen-specific and antigen-independent factors.
Ishigatsubo
Y
Y
Yokohama City University Medical School, Japan.
Sakamoto
H
H
Hagiwara
E
E
Aoki
A
A
Shirai
A
A
Tani
K
K
Okubo
T
T
Klinman
D M
DM
D000199:Actins / Q000276:immunology; D000328:Adult; D000974:Antibodies, Antinuclear / Q000032:analysis / Q000276:immunology; D000918:Antibody Specificity; D001323:Autoantibodies / Q000032:analysis* / Q000276:immunology; D001324:Autoantigens / Q000276:immunology*; D001327:Autoimmune Diseases / Q000097:blood* / Q000276:immunology / Q000473:pathology; D001402:B-Lymphocytes / Q000276:immunology / Q000473:pathology*; D002308:Cardiolipins / Q000276:immunology; D004247:DNA / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007958:Leukocyte Count / Q000379:methods*; D008180:Lupus Erythematosus, Systemic / Q000097:blood* / Q000276:immunology / Q000473:pathology; D008213:Lymphocyte Activation; D008297:Male; D009218:Myosins / Q000276:immunology; D014168:Transferrin / Q000276:immunology
D000199:Actins; D000974:Antibodies, Antinuclear; D001323:Autoantibodies; D001324:Autoantigens; D002308:Cardiolipins; D014168:Transferrin; D004247:DNA; D009218:Myosins