title: Stimulation of androgen-dependent gene expression by the adrenal precursors dehydroepiandrosterone and androstenedione in the rat ventral prostate.
abstract: Androgens play a major role in the development, growth, and function of accessory sexual organs, especially the prostate. However, the testis is not the sole source of circulating androgens in man, since the adrenal gland secretes dehydroepiandrosterone (DHEA), DHEA sulfate, and androstenedione (delta 4-dione) in large quantities. The aim of the present study was to investigate the effect of plasma concentrations of DHEA and delta 4-dione similar to those found in adult man on sensitive and specific markers of androgen action in the rat ventral prostate. In addition to ventral prostate weight, we have measured the steady state levels of the mRNAs encoding the C1 component of rat prostatic binding protein (PBP-C1) and spermine-binding protein (SBP) using 35S-labeled cDNA probes for in situ hybridization. One week after castration, ventral prostate weight fell 84%, while prostatic 5 alpha-dihydrotestosterone (DHT) and androgen-dependent mRNAs were undetectable. When administered via Silastic implants to castrated adult rats for 1 week, plasma concentrations of 1.37 +/- 0.06 ng/ml DHEA or 0.43 +/- 0.08 ng/ml delta 4-dione independently caused increases in ventral prostate weight to 33% and 65% of normal values, respectively. The same plasma levels of DHEA and delta 4-dione resulted in high intraprostatic levels of DHT to 1.19 +/- 0.34 and 3.66 +/- 0.89 ng/g tissue, respectively. Furthermore, DHEA caused an increase in the steady state levels of PBP-C1 and SBP mRNAs to 50% and 57% of the normal state, respectively, while delta 4-dione caused increases corresponding to 80% and 119% of control values, respectively. Castrated adult rats receiving testosterone at a concentration of 1.66 +/- 0.37 ng/ml plasma maintained normal ventral prostate weight and gene expression levels. The present results demonstrate that circulating levels of the adrenal steroids DHEA and delta 4-dione comparable to those found in man cause an important stimulation of androgen-dependent gene expression in the rat, probably after their conversion to DHT in the prostatic tissue itself.
authors.affiliation: Medical Research Council Group in Molecular Endocrinology, Research Center, Laval University Medical Center, Quebec, Canada.
authors.forename: C
authors.initials: C
authors.lastname: Labrie
authors.forename: J
authors.initials: J
authors.lastname: Simard
authors.forename: H F
authors.initials: HF
authors.lastname: Zhao
authors.forename: A
authors.initials: A
authors.lastname: Belanger
authors.forename: G
authors.initials: G
authors.lastname: Pelletier
authors.forename: F
authors.initials: F
authors.lastname: Labrie
mesh_terms: D000311:Adrenal Glands / Q000502:physiology*; D000727:Androgen-Binding Protein / Q000235:genetics*; D000728:Androgens / Q000097:blood; D000735:Androstenedione / Q000494:pharmacology*; D000818:Animals; D001345:Autoradiography; D002352:Carrier Proteins / Q000235:genetics*; D003687:Dehydroepiandrosterone / Q000494:pharmacology*; D005485:Flutamide / Q000494:pharmacology; D005796:Genes; D047908:Intracellular Signaling Peptides and Proteins; D007700:Kinetics; D008297:Male; D009919:Orchiectomy; D011467:Prostate / Q000187:drug effects / Q000378:metabolism*; D060789:Prostatein; D012333:RNA, Messenger / Q000187:drug effects* / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D060765:Secretoglobins; D013462:Sulfur Radioisotopes; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects*; D014598:Uteroglobin; D060788:Mammaglobin B
chemical_list: D000727:Androgen-Binding Protein; D000728:Androgens; D000735:Androstenedione; D002352:Carrier Proteins; D003687:Dehydroepiandrosterone; D005485:Flutamide; D047908:Intracellular Signaling Peptides and Proteins; D060789:Prostatein; D012333:RNA, Messenger; D060765:Secretoglobins; D013462:Sulfur Radioisotopes; D014598:Uteroglobin; C032603:Sbp protein, rat; C511484:Scgb1d2 protein, rat; C511483:Scgb1d4 protein, rat; C556468:Scgb2a1 protein, rat; D060788:Mammaglobin B