Effect of brain-gut peptides upon neurons in centrally regulating sites for drinking.
The effects of angiotensin II (A II) and ANP on spontaneously active neurons in the subfornical organ (SFO), anteroventral third ventricle (AV3V) and supraoptic (SON) and paraventricular nucleus (PVN) were investigated using slice preparations and extracellular recordings. Application of A II (10(-7)M) excited the neural activity of 66% of the SFO neurons, 28% of the AV3V neurons and 44% of the SON neurons. The threshold concentration to produce responses in SFO and AV3V neuron was less than 10(-10)M, while that in SON neurons was 10(-9)M. The excitatory effects of A II were reversibly antagonized by saralasin and persisted after synaptic blockade in a low Ca2+ and high Mg2+ medium. Application of ANP (10(-7)M) inhibited the neural activity of 41% of the AV3V neurons, 22% of the PVN neurons and only 14% of the SFO neurons but had no effect on SON neurons. The threshold concentration for ANP in the AV3V was 10(-11)M. Interestingly, ANP inhibited A II induced excitation in most of the SFO neurons (87%), while ANP had little effects on their spontaneous firing rates. These results show that both peptides of A II and ANP have direct central actions on hypothalamic neurons although ANP can not directly influence magnocellular neurons, suggesting that these blood borne peptides are detected in the SFO and AV3V and that they are acting as a neurotransmitter or a neuromodulator in the central nervous system to regulate water homeostasis.
Yamashita
H
H
Department of Physiology, University of Occupational and Environmental Health, School of Medicine, Kitakyushu, Japan.
Inenaga
K
K
Okuya
S
S
Hattori
Y
Y
Yamamoto
S
S
D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D002320:Cardiovascular Physiological Phenomena; D002319:Cardiovascular System / Q000187:drug effects; D002552:Cerebral Ventricles / Q000166:cytology / Q000187:drug effects; D004326:Drinking / Q000187:drug effects*; D008297:Male; D009474:Neurons / Q000187:drug effects*; D010286:Paraventricular Hypothalamic Nucleus / Q000166:cytology / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D013356:Subfornical Organ / Q000166:cytology / Q000187:drug effects; D013495:Supraoptic Nucleus / Q000166:cytology / Q000187:drug effects; D014667:Vasopressins / Q000378:metabolism
D014667:Vasopressins; D000804:Angiotensin II; D009320:Atrial Natriuretic Factor