title: Effects of calcium and EDTA on rat skin capillary permeability and on its response to histamine, serotonin and bradykinin.
abstract: Evans blue extravasation in rat skin was used to study the effects of Ca2+ and EDTA on vascular permeability and on its response to mediators of inflammation. Ca2+ induced a concentration-dependent decrease of vascular permeability. The opposite effect was seen with EDTA 0.2 mM or higher. Effects on vascular permeability of intradermically injected histamine 100 micrograms/ml, serotonin 5 micrograms/ml and bradykinin 5 micrograms/ml, were lower when Ca2+ 8 mM was injected in the same site, and higher when EDTA 2 mM was given. EDTA effects were inhibited by Ca2+. The results suggest that, in rat skin, Ca2+ decreases capillary permeability and its response to histamine, serotonin and bradykinin.
authors.affiliation: Cátedra de Farmacología, Facultad de Odontología, UBA, Argentina.
authors.forename: L
authors.initials: L
authors.lastname: Busch
authors.forename: J
authors.initials: J
authors.lastname: Tessler
authors.forename: P M
authors.initials: PM
authors.lastname: Bazerque
mesh_terms: D000818:Animals; D001920:Bradykinin / Q000037:antagonists & inhibitors*; D002118:Calcium / Q000494:pharmacology*; D002199:Capillary Permeability / Q000187:drug effects*; D004492:Edetic Acid / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000502:physiology; D005070:Evans Blue; D005260:Female; D006633:Histamine Antagonists / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012702:Serotonin Antagonists / Q000494:pharmacology*; D012867:Skin / Q000098:blood supply*
chemical_list: D006633:Histamine Antagonists; D012702:Serotonin Antagonists; D005070:Evans Blue; D004492:Edetic Acid; D001920:Bradykinin; D002118:Calcium