Large scale preparation of bacteriophage lambda by tangential flow ultrafiltration for isolation of lambda DNA.
A preparative procedure for purifying bacteriophage lambda from large volumes of phage lysates by recirculating tangential flow ultrafiltration is described. Lambda DNA, isolated by deproteinization of the phage, is suitable for use in molecular biology.
Rembhotkar
G W
GW
Genetic Engineering Division, CSIR Centre for Biochemicals, Delhi, India.
Khatri
G S
GS
D010582:Bacteriophage lambda / Q000302:isolation & purification*; D004279:DNA, Viral / Q000302:isolation & purification*; D008567:Membranes, Artificial; D008970:Molecular Weight; D014462:Ultrafiltration
D004279:DNA, Viral; D008567:Membranes, Artificial