Production of antibodies against the coenzyme pyrrolequinoline quinone.
Polyclonal antibodies against pyrrolequinoline quinone have been elicited in rabbits. These antibodies react with free and protein-bound pyrrolequinoline quinone. In particular they react with native and denatured lentil seedling amine oxidase as detected by dot-blot and ELISA assays. The presence of 1 mol pyrrolequinoline quinone per mol of enzyme was determined by the last method.
Citro
G
G
Istituto Regina Elena per la Ricerca sul Cancro, Roma, Italy.
Verdina
A
A
Galati
R
R
Floris
G
G
Sabatini
S
S
Finazzi-Agrò
A
A
D006631:Amine Oxidase (Copper-Containing); D000906:Antibodies / Q000276:immunology; D000941:Antigens / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D007887:Fabaceae; D006358:Hot Temperature; D015151:Immunoblotting; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000032:analysis*; D045542:PQQ Cofactor; D010944:Plants / Q000201:enzymology*; D010946:Plants, Medicinal; D011489:Protein Denaturation; D015363:Quinolones / Q000032:analysis* / Q000276:immunology
D000906:Antibodies; D000941:Antigens; D015363:Quinolones; D045542:PQQ Cofactor; D006631:Amine Oxidase (Copper-Containing); D000587:Oxidoreductases Acting on CH-NH Group Donors