Quisqualate receptor-mediated rhythmic bursting of rat hypothalamic neurons in dissociated cell culture.
Dissociated hypothalamic neurons from embryonic rat brain exhibit a level of spontaneous synaptic activity after 21 days in culture. When GABA-mediated responses are blocked by picrotoxin or bicuculline (20 microM), the neurons burst rhythmically. Rhythmic burst activity is generated in most cells by postsynaptic excitatory currents (EPSCs) through non-specific cationic channels rather than by intrinsic pacemaker currents. We present evidence that EPSCs are mediated by an excitatory amino acid and a quisqualate receptor type.
Misgeld
U
U
Max-Planck-Institute for Psychiatry, Department of Neurophysiology, Planegg-Martinsried, F.R.G.
Swandulla
D
D
D000200:Action Potentials / Q000187:drug effects; D000596:Amino Acids / Q000502:physiology; D000818:Animals; D002478:Cells, Cultured; D018756:GABA Antagonists; D007031:Hypothalamus / Q000196:embryology / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D051381:Rats; D018091:Receptors, AMPA; D017981:Receptors, Neurotransmitter / Q000502:physiology*; D005680:gamma-Aminobutyric Acid / Q000502:physiology
D000596:Amino Acids; D018756:GABA Antagonists; D018091:Receptors, AMPA; D017981:Receptors, Neurotransmitter; D005680:gamma-Aminobutyric Acid