Molecular distribution of gold(I) in human blood plasma after treatment with the antiarthritic disodium aurothiomalate.
On-line separation and detection of both high- and low-molecular-weight gold species in human blood plasma treated with disodium aurothiomalate were achieved by a technique incorporating a combination of fast protein liquid chromatography with continuous monitoring of absorbance at 254 nm and eluant gold concentration by flame atomic absorption spectroscopy. The fate of gold (40 microM disodium aurothiomalate) in human blood plasma was monitored over a period of 24 h. Results obtained indicated that more than 50% of the gold is associated with a peak identified as albumin after a 5 min incubation period at 37 degrees C, and by 30 min more than 90% is found to be associated with albumin. This novel analytical combination enables the rapid, selective and sensitive determination of gold(I) and its biotransformation products (both protein--and non-protein-bound) in biological fluids.
Rayner
M H
MH
Department of Chemistry, Birkbeck College, University of London, England.
Grootveld
M
M
Sadler
P J
PJ
D000328:Adult; D001168:Arthritis / Q000188:drug therapy; D001711:Biotransformation; D002850:Chromatography, Gel; D002853:Chromatography, Liquid; D006046:Gold / Q000097:blood*; D006052:Gold Sodium Thiomalate / Q000493:pharmacokinetics* / Q000627:therapeutic use; D006801:Humans; D008297:Male; D010949:Plasma / Q000032:analysis; D013054:Spectrophotometry, Atomic
D006052:Gold Sodium Thiomalate; D006046:Gold