title: Reagin production in mice: effect of subcutaneous and oral sensitization with untreated bovine milk and homogenized bovine milk.
abstract: Subcutaneous and oral sensitization with untreated bovine milk and homogenized bovine milk in mice was analyzed in two different sensitization schemes--a low dose and a high dose scheme. Homologous murine passive cutaneous anaphylaxis test was used to measure the titres of reagins (IgE and IgG1). Subcutaneous and oral sensitization with low doses of homogenized milk induced production of higher titres of IgE than sensitization with high doses of homogenized milk. By contrast, sensitization with both low and high doses of untreated bovine milk resulted in very low or undetectable IgE titres. The results indicate that homogenization of milk increases the efficacy of inducing IgE production in mice. Some possible implications of these results with respect to clinical allergy are discussed.
authors.affiliation: Department of Veterinary Pathology, Royal Veterinary and Agricultural University, Copenhagen, Denmark.
authors.forename: B R
authors.initials: BR
authors.lastname: Nielsen
authors.forename: O M
authors.initials: OM
authors.lastname: Poulsen
authors.forename: J
authors.initials: J
authors.lastname: Hau
mesh_terms: D000818:Animals; D000917:Antibody Formation; D002417:Cattle; D005260:Female; D005511:Food Handling; D005512:Food Hypersensitivity; D006967:Hypersensitivity; D007116:Immunization, Passive; D007073:Immunoglobulin E / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis* / Q000145:classification; D051379:Mice; D008815:Mice, Inbred Strains; D008892:Milk / Q000276:immunology*
chemical_list: D007074:Immunoglobulin G; D007073:Immunoglobulin E