Lack of evidence for strand-specific inhibition of poliovirus RNA synthesis by 3-methylquercetin.
Buffalo Green Monkey cells were infected with poliovirus-1 in the presence of 3-methylquercetin or guanidine, and the formation of positive- and negative-strand viral RNA was monitored using single-stranded RNA probes. Both 3-methylquercetin and guanidine prevented the formation of plus-strand as well as minus-strand viral RNA, although, due to the high multiplicity of infection used, a high number of genomic viral RNA was always present in the cells.
Lopez Pila
J M
JM
Institut für Wasser-, Boden- und Lufthygiene, Bundesgesundheitsamt, Berlin, F.R.G.
Kopecka
H
H
Vanden Berghe
D
D
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004587:Electrophoresis, Agar Gel; D005419:Flavonoids / Q000494:pharmacology*; D044948:Flavonols; D015870:Gene Expression; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D017955:Poliovirus / Q000187:drug effects* / Q000235:genetics; D011794:Quercetin / Q000031:analogs & derivatives / Q000494:pharmacology*; D015347:RNA Probes; D012330:RNA, Double-Stranded / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis / Q000187:drug effects*
D000998:Antiviral Agents; D005419:Flavonoids; D044948:Flavonols; D015347:RNA Probes; D012330:RNA, Double-Stranded; D012367:RNA, Viral; C047368:3-methylquercetin; D011794:Quercetin