Characterization of a cDNA clone encoding the visna virus transactivating protein.
The unique pathogenesis of lentiviral infections in humans and ruminant animals may be explained, in part, by the complex mechanisms regulating transcription and translation of their viral genes. This report demonstrates that a visna virus-encoded protein transactivates viral gene expression. A 1.4-kilobase cDNA clone encodes two distinct proteins with apparent molecular masses of 21.5 and 10 kDa. We demonstrate that the 10-kDa species is the visna virus transactivating (Tat) protein; the other species may be analogous to the rev (formerly art or trs) gene product of human immunodeficiency virus.
Davis
J L
JL
Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Clements
J E
JE
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics*; D004742:Enhancer Elements, Genetic; D005091:Exons; D005786:Gene Expression Regulation; D015696:Gene Products, tat; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic; D014162:Transfection; D014790:Visna-maedi virus / Q000235:genetics*
D004279:DNA, Viral; D015696:Gene Products, tat; D009856:Oncogene Proteins, Viral; D012367:RNA, Viral; D014157:Transcription Factors