title: [NAD participation in protecting DNA from damaging factors].
abstract: It was found that autolytic degradation of DNA during the isolation of nuclei under conditions of an in situ stimulated NAD biosynthesis is decreased to 2.5% in comparison with 5.2% in control. The increase in the NAD-pyrophosphorylase activity and the intranuclear content of NAD was almost 3-fold, whereas the amount of the acid-soluble DNA material was decreased 2-fold. The degree of DNA damage was increased up to 23% after induction with ethidium bromide. Under these conditions a preliminary in vivo stimulation of NAD biosynthesis by nicotinamide diminished the amount of the acid-soluble material. The levels of intranuclear NAD as well as the NAD-pyrophosphorylase and poly-(ADP-riboso)polymerase activities were essentially affected.
authors.forename: N A
authors.initials: NA
authors.lastname: Muliavko
authors.forename: A V
authors.initials: AV
authors.lastname: Petik
authors.forename: G V
authors.initials: GV
authors.lastname: Donchenko
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology; D002645:Chickens; D002843:Chromatin / Q000378:metabolism; D004249:DNA Damage; D004996:Ethidium / Q000494:pharmacology; D008099:Liver / Q000201:enzymology; D009243:NAD / Q000096:biosynthesis / Q000378:metabolism*; D009536:Niacinamide / Q000494:pharmacology
chemical_list: D002843:Chromatin; D009243:NAD; D009536:Niacinamide; D004996:Ethidium
vernacular_title: Uchastie NAD v predokhranenii DNK ot povrezhdaiushchikh faktorov.