Retrovirus-induced insertional mutation in Mov13 mice affects collagen I expression in a tissue-specific manner.
In the Mov13 mouse mutant, transcription of the alpha 1 (1) collagen gene is blocked by a retroviral insert in the first intron. We now report that teeth derived from homozygous embryos produce a dentin layer containing normal amounts of collagen 1. In situ hybridization and RNAase protection experiments indicate that the mutant allele is efficiently transcribed in odontoblasts, in contrast to other cell types. Correct splicing of the primary transcript containing the viral sequence results in a functional alpha 1 (1) collagen mRNA. The absence of a mutagenic effect in odontoblasts, as opposed to fibroblasts, suggests that the retroviral insert interferes with tissue-specific transcriptional control of the alpha 1 (1) collagen gene, most likely by inactivating cell-type-specific cis-acting regulatory elements.
Kratochwil
K
K
Institute of Molecular Biology, Austrian Academy of Sciences, Salzburg.
von der Mark
K
K
Kollar
E J
EJ
Jaenisch
R
R
Mooslehner
K
K
Schwarz
M
M
Haase
K
K
Gmachl
I
I
Harbers
K
K
D000818:Animals; D003094:Collagen / Q000235:genetics*; D004251:DNA Transposable Elements; D005796:Genes; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D008979:Moloney murine leukemia virus / Q000235:genetics*; D009154:Mutation; D009693:Nucleic Acid Hybridization; D009804:Odontoblasts / Q000378:metabolism; D009805:Odontogenesis; D009928:Organ Specificity; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D014083:Tooth Germ / Q000637:transplantation; D014158:Transcription, Genetic
D004251:DNA Transposable Elements; D012333:RNA, Messenger; D003094:Collagen