Quantitative analysis of demeclocycline by high-performance liquid chromatography.
A high-performance liquid chromatographic (HPLC) method suitable for the quality control of demeclocycline is described. The stationary phase is a poly(styrene-divinylbenzene) copolymer, kept at 60 degrees C. The mobile phase comprises 2-methyl-2-propanol-0.2 M potassium phosphate buffer (pH 9.0)-0.02 M tetrabutylammonium hydrogen sulphate (pH 9.0)-0.01 M sodium edetate (pH 9.0)-water (8:10:15:10:57, m/v/v/v/v). The flow rate is 1 ml min-1 and detection is performed at 254 nm. Official standards are compared and results for the analysis of a number of commercial bulk samples and preparations are presented. 4-Epidemeclocycline and demethyltetracycline are the main impurities. 4-Epidemethyltetracycline and 2-acetyl-2-decarboxamido-demeclocycline can also be present.
Weng
N D
ND
Katholieke Universiteit Leuven, Laboratorium voor Farmaceutische Wetenschappen, Belgium.
Roets
E
E
Hoogmartens
J
J
D002214:Capsules; D002851:Chromatography, High Pressure Liquid; D003707:Demeclocycline / Q000031:analogs & derivatives / Q000032:analysis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D009824:Ointments; D011137:Polystyrenes; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet; D014867:Water / Q000032:analysis
D002214:Capsules; D007202:Indicators and Reagents; D009824:Ointments; D011137:Polystyrenes; D014867:Water; D003707:Demeclocycline; C003771:Amberlite XAD-2 resin