Improved radioimmunoassay of atrial natriuretic peptide in plasma.
We describe a radioimmunoassay (RIA) for measurement of atrial natriuretic peptide (ANP), based on one-step incubation and a simplified extraction procedure. The extraction was performed on a "Supelclean LC 18" column, with 2-mL plasma samples. Use of a diiodinated tracer improved the sensitivity of the RIA method. The minimal detectable value was 5 ng/L. Simplification of the extraction procedure and simultaneous incubation of the reagents provide a method more suitable for routine standard assay of ANP than those currently available. Intra- and interassay CVs were 6% (n = 12) and 11% (n = 10), respectively. The mean concentration of ANP in plasma of 32 healthy volunteers was 33 (SEM 4) ng/L. The ANP values for plasma after one-step incubation correlated well with those determined by a commercial RIA kit: r = 0.971, slope = 1.099, intercept = 1.949 ng/L (n = 25).
Poiesi
C
C
Department of Chemistry, School of Medicine, University of Brescia, Italy.
Rodella
A
A
Mantero
G
G
Cannella
G
G
Ferrari
R
R
Albertini
A
A
D009320:Atrial Natriuretic Factor / Q000097:blood* / Q000592:standards; D001800:Blood Specimen Collection; D006801:Humans; D011863:Radioimmunoassay; D011933:Reagent Kits, Diagnostic; D013696:Temperature
D011933:Reagent Kits, Diagnostic; D009320:Atrial Natriuretic Factor