[Immunoenzyme analysis of the change in the expression of class II antigens of the major histocompatibility complex under the influence of interferon preparations].
The interferons (IFN's) are natural regulators of the immune response. This quality of IFN depends to a large extent on their capacity to change the expression of the antigens of the second (II) class of the Major Histocompatibility Complex. The most effective modulator of the expression of the antigens of the class II MHC is beta-IFN. The aim of this work is to explore with the aid of enzyme-linked immunosorbent assay (ELISA) peculiarities of an action of the preparations alpha- and beta-IFN's of a man on the expression of the antigens of the second class on macrophages of mice with using monoclonal antibodies to the products of subregions I-A and I-E MHC. As a result of exploration we have the following: both preparations IFN's (leukinterferon and beta-IFN) reinforce the expression of the antigens of the second class on the macrophages of mice spleens. With combined influence preparation alpha-IFN and beta-IFN show interweakened effect on the expression of the antigens of the class MHC. Exposed regularities of the expression have the same type for products of subregions I-A and I-E H-2 of mice complex. ELISA is the sensible method of determination of a level of the expression of the antigens of the class II MHC on the cell's populations.
Kremlev
S G
SG
Medunitsyn
N V
NV
D000818:Animals; D000911:Antibodies, Monoclonal; D004797:Enzyme-Linked Immunosorbent Assay; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology*; D008264:Macrophages / Q000276:immunology; D008285:Major Histocompatibility Complex / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000276:immunology
D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II; D007370:Interferon Type I; D007371:Interferon-gamma
Immunofermentnyĭ analiz izmeneniia ékspressii antigenov II klassa glavnogo kompleksa gistosovmestimosti pod vliianiem preparatov interferona.