Characterization and expression of the rat heart sarcoplasmic reticulum Ca2+-ATPase mRNA.
Sarcoplasmic reticulum Ca2+-ATPase cDNA clones have been isolated from an adult rat heart cDNA library and the nucleotide sequence of the Ca2+-ATPase mRNA determined. The sequence has an open reading frame of 997 codons. It is identical to a cDNA isolated from a rat stomach cDNA library and 90% isologous to the rabbit and human slow/cardiac cDNAs. Nuclease S1 mapping analysis indicates that this sequence corresponds to the main Ca2+-ATPase mRNA present in heart and in slow skeletal muscle and that it is expressed in various proportions in smooth and non-muscle tissues, together with another isoform which differs from the cardiac form in the sequence of its 3'-end.
Lompre
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AM
INSERM U127, Hôpital Lariboisière, Paris, France.
de la Bastie
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D
Boheler
K R
KR
Schwartz
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D000252:Calcium-Transporting ATPases / Q000235:genetics* / Q000302:isolation & purification; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D004720:Endonucleases; D008297:Male; D008969:Molecular Sequence Data; D009130:Muscle, Smooth / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology*; D012333:RNA, Messenger / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains; D012519:Sarcoplasmic Reticulum / Q000201:enzymology*; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013270:Stomach / Q000201:enzymology
D012333:RNA, Messenger; D004247:DNA; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases; D000252:Calcium-Transporting ATPases