Detection of human papillomavirus DNA in fine-needle aspirations of metastatic squamous-cell carcinoma of the uterine cervix using the polymerase chain reaction.
Routinely processed fine-needle aspirations of metastatic squamous-cell carcinoma (SCC) were analyzed for human papillomavirus type 16 (HPV-16) using the polymerase chain reaction (PCR), an in vitro DNA amplification method. HPV-16 DNA was detected in five of seven (71%) metastases from SCC of the uterine cervix. In two cases in which the primary tumor was available for comparison, the HPV-16 DNA content of the primary tumor and of the metastasis was identical. HPV-16 was not found in a metastatic SCC from the lung or in a metastatic nasopharyngeal SCC. These findings demonstrate that HPV-16 DNA sequences can be readily detected in routinely processed fine-needle aspirations using the polymerase chain reaction. The finding of HPV-16 DNA in a metastasis may serve to direct a search for a primary site of origin.
Shibata
D
D
Department of Pathology, Los Angeles County-University of Southern California Medical Center 90033.
Cosgrove
M
M
Arnheim
N
N
Martin
W J
WJ
Martin
S E
SE
D000293:Adolescent; D000328:Adult; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000556:secondary; D004279:DNA, Viral / Q000032:analysis*; D004259:DNA-Directed DNA Polymerase; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000556:secondary
D004279:DNA, Viral; D004259:DNA-Directed DNA Polymerase