Phagocytic properties of hepatic endothelial cells and splenic macrophages compensating for a decreased phagocytic function of Kupffer cells in the chronically ethanol-fed rats.
The balance of phagocytic function among Kupffer cells, hepatic endothelial cells and splenic macrophages in the chronically ethanol-fed rats has been investigated. Clearance of latex particles in the blood was measured to estimate the function of the reticuloendothelial system. Phagocytosis of latex particles by Kupffer cells, hepatic endothelial cells or splenic macrophages in vivo was measured by counting the number of ingested particles in a cell after isolation of hepatic nonparenchymal cells or spleen cells following injection of different amounts of latex particles. Latex particle clearance was suppressed in the ethanol-fed rats, demonstrating a decreased phagocytic capacity of the reticuloendothelial system. Markedly decreased phagocytic function was found in 40% of Kupffer cells of the chronically ethanol-fed rats. In contrast, the number of latex particles in hepatic endothelial cells and in splenic macrophages was increased after injection of a triggering dose of latex particles. From these results it may be concluded that an increased phagocytosis of hepatic endothelial cells and splenic macrophages could compensate for the decreased phagocytic function of Kupffer cells.
Shiratori
Y
Y
2nd Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Jin'nai
H
H
Teraoka
H
H
Matano
S
S
Matsumoto
K
K
Kamii
K
K
Tanaka
M
M
Okano
K
K
D000818:Animals; D004195:Disease Models, Animal; D004727:Endothelium / Q000187:drug effects; D000431:Ethanol / Q000097:blood / Q000633:toxicity*; D007728:Kupffer Cells / Q000187:drug effects*; D007840:Latex; D008099:Liver / Q000166:cytology / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects*; D008297:Male; D008863:Microspheres; D010587:Phagocytosis / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000166:cytology / Q000187:drug effects*
D007840:Latex; D000431:Ethanol