title: Effects of dietary supplementation with cod liver oil on monocyte thromboplastin synthesis, coagulation and fibrinolysis.
abstract: In a controlled trial 40 healthy persons (20 men and 20 women) were tested before and after a daily supplement with 25 ml cod liver oil for 8 weeks. The diet increased the eicosapentaenoic acid (20:5 n-3) content in serum and monocytes four- to five-fold whereas the arachidonic acid (20:4 n-6) content decreased 10-20% in both serum and monocytes. Stimulation of blood with a low concentration of lipopolysaccharides (LPS) revealed a 40% suppression of LPS-induced thromboplastin synthesis in the monocytes after 8 weeks of CLO intake. In the same LPS stimulation system, men were found to generate significantly more thromboxane B2 than women (4.9 ng ml-1 versus 3.4 ng ml-1). After the CLO supplementation for 8 weeks the thromboxane B2 was reduced by a mean of 70% in women and 60% in men. Factor VII and fibrinogen appeared to be unaltered by CLO intake. Determination of whole blood clot lysis time and tissue plasminogen activator (t-PA) did not indicate any significant influence of n-3 fatty acids on fibrinolysis.
authors.affiliation: Institute of Medical Biology, University of Tromsø, Norway.
authors.forename: J B
authors.initials: JB
authors.lastname: Hansen
authors.forename: J O
authors.initials: JO
authors.lastname: Olsen
authors.forename: L
authors.initials: L
authors.lastname: Wilsgård
authors.forename: B
authors.initials: B
authors.lastname: Osterud
mesh_terms: D000328:Adult; D003060:Cod Liver Oil / Q000008:administration & dosage / Q000494:pharmacology*; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage / Q000494:pharmacology*; D015118:Eicosapentaenoic Acid / Q000097:blood; D005167:Factor VII / Q000096:biosynthesis; D005227:Fatty Acids / Q000097:blood; D005260:Female; D005342:Fibrinolysis / Q000187:drug effects*; D005395:Fish Oils / Q000494:pharmacology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D008042:Linolenic Acids / Q000494:pharmacology; D008297:Male; D013925:Thromboplastin / Q000096:biosynthesis*; D013929:Thromboxane B2 / Q000032:analysis; D010959:Tissue Plasminogen Activator / Q000032:analysis
chemical_list: D004042:Dietary Fats, Unsaturated; D005227:Fatty Acids; D005395:Fish Oils; D008042:Linolenic Acids; D013929:Thromboxane B2; D003060:Cod Liver Oil; D005167:Factor VII; D013925:Thromboplastin; D015118:Eicosapentaenoic Acid; D010959:Tissue Plasminogen Activator