Accessory signals in murine cytolytic T cell responses. Dual requirement for IL-1 and IL-6.
We have examined the nature of the accessory factors required for the development of primary murine CTL in accessory cell-depleted allogeneic mixed lymphocyte cultures. None of the known macrophage-derived factors was active by itself but the combination of IL-1 and IL-6 induced cytolytic responses equivalent to those of unfractionated cultures. Experiments with purified T cell subsets and antibodies blocking the action of IL-2 and IL-4 demonstrated that IL-1 and IL-6 acted directly on CD8+ cells capable of growth factor production. Analysis of the respective roles of IL-1 and IL-6 indicated that IL-6 acts before IL-1 and is capable apparently by itself to induce responsiveness to IL-2. By contrast, production of IL-2 required the additional presence of IL-1.
Renauld
J C
JC
International Institute of Cellular and Molecular Pathology, Université Catholique de Louvain, Brussels, Belgium.
Vink
A
A
Van Snick
J
J
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D000945:Antigens, Differentiation, T-Lymphocyte; D016827:CD8 Antigens; D003602:Cytotoxicity, Immunologic; D007375:Interleukin-1 / Q000502:physiology*; D007376:Interleukin-2 / Q000096:biosynthesis / Q000502:physiology; D015850:Interleukin-6; D007378:Interleukins / Q000502:physiology*; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D010641:Phenotype; D013602:T-Lymphocytes, Cytotoxic / Q000145:classification / Q000276:immunology*
D000945:Antigens, Differentiation, T-Lymphocyte; D016827:CD8 Antigens; D007375:Interleukin-1; D007376:Interleukin-2; D015850:Interleukin-6; D007378:Interleukins