Two-dimensional gel electrophoresis of RNase T1 resistant oligonucleotides of flavivirus RNA using ultrathin gels.
A convenient method employing a commercially available apparatus for two-dimensional gel electrophoresis of RNase T1 resistant oligonucleotides using ultrathin gels has been developed. The methodology overcomes problems commonly associated with the establishment of good, bubble-free, fusion of the first and second dimension gels. The use of ultrathin gels results in autoradiograms with well resolved oligonucleotide spots.
Coelen
R J
RJ
Department of Microbiology, University of Western Australia, Nedlands.
Flynn
L M
LM
Mackenzie
J S
JS
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005416:Flavivirus / Q000302:isolation & purification*; D012367:RNA, Viral / Q000302:isolation & purification*; D006163:Ribonuclease T1; D014773:Virology / Q000379:methods
D012367:RNA, Viral; D006163:Ribonuclease T1