Identification of a specific receptor for interleukin-1 on rat bone marrow cells.
The interleukin-1 (IL-1) receptor on rat bone marrow cells was investigated using 125I-labeled IL-1 alpha and -1 beta. These radiolabeled ligands bound to the rat bone marrow cells in a specific and saturable manner with Kd values of 0.36 +/- 0.072 nM and 1.9 +/- 0.27 nM, respectively. In a competitive binding experiment, IL-1 alpha and -1 beta inhibited the binding of 125I-IL-1 alpha with Ki values of 0.35 +/- 0.041 nM and 2.9 +/- 1.0 nM. The binding of 125I-IL-1 beta was inhibited by IL-1 alpha and -1 beta with Ki values of 0.27 +/- 0.020 nM and 0.74 +/- 0.12 nM. In cross-linking experiments, 125I-IL-1 alpha was covalently incorporated into two proteins of 163 kDa and 63 kDa. These results suggested the presence of two binding proteins for IL-1 on the rat bone marrow cells.
Mizuno
K
K
Cellular Technology Institute, Otsuka Pharmaceutical Co. Ltd, Tokushima, Japan.
Nakai
S
S
Ohmoto
Y
Y
Hirai
Y
Y
D000818:Animals; D001667:Binding, Competitive; D001853:Bone Marrow / Q000276:immunology*; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism*; D007700:Kinetics; D008970:Molecular Weight; D051381:Rats; D011971:Receptors, Immunologic / Q000302:isolation & purification / Q000378:metabolism*; D017472:Receptors, Interleukin-1; D011994:Recombinant Proteins / Q000378:metabolism
D007375:Interleukin-1; D011971:Receptors, Immunologic; D017472:Receptors, Interleukin-1; D011994:Recombinant Proteins