Localization of intracellular zinc under conditions of altered permeability control of the plasma membrane.
Intracellular zinc was located as electron dense granules associated with the plasma membrane, endoplasmic reticular membranes, mitochondrial membranes, nuclear membranes and chromatin in Zajdela ascitic hepatoma and AK5 macrophage ascitic tumour cells. The quantity of intracellular zinc estimated by atomic emission spectrometer was different in the two cell lines. However, after loss of permeability control by the plasma membrane, involving glutaraldehyde and heat-shock treatments, the quantity of intracellular zinc was increased to almost the same extent in both cases.
Reddy
A G
AG
Centre for Cellular and Molecular Biology, Hyderabad, India.
Devi
B G
BG
Rao
S B
SB
Gupta
P D
PD
D000818:Animals; D002460:Cell Line; D002463:Cell Membrane Permeability / Q000187:drug effects; D005404:Fixatives; D005976:Glutaral / Q000494:pharmacology; D006358:Hot Temperature; D008114:Liver Neoplasms, Experimental / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron; D051381:Rats; D013054:Spectrophotometry, Atomic; D014407:Tumor Cells, Cultured / Q000378:metabolism* / Q000648:ultrastructure; D015032:Zinc / Q000032:analysis*
D005404:Fixatives; D015032:Zinc; D005976:Glutaral