title: Oxidative metabolism of the bovine alveolar macrophage.
abstract: Oxidative respiratory burst activity was examined in lavage-procured bovine pulmonary alveolar macrophages. Nonstimulated alveolar macrophages released a minimal quantity of superoxide anion and had small amounts of glucose flux through the pathways of energy metabolism. Nonstimulated cells metabolized substantial amounts of glucose through the hexose monophosphate shunt. Stimulation with opsonized zymosan particles induced a tenfold increase in the release of superoxide anion and a twofold increase in the flux of glucose through the hexose monophosphate shunt and the pathways of energy metabolism. Preliminary observations also indicated that the magnitude of the burst varied between sets of bronchoalveolar cells obtained from the same calf over time.
authors.affiliation: Department of Clinical Studies, College of Veterinary Medicine, University of Pennsylvania, New Bolton Center, Kennet Square 19348.
authors.forename: R M
authors.initials: RM
authors.lastname: Dyer
authors.forename: S
authors.initials: S
authors.lastname: Erney
authors.forename: P
authors.initials: P
authors.lastname: Spencer
authors.forename: C E
authors.initials: CE
authors.lastname: Benson
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002417:Cattle / Q000276:immunology*; D002952:Citric Acid Cycle; D005947:Glucose / Q000378:metabolism*; D006600:Hexosephosphates / Q000378:metabolism; D008264:Macrophages / Q000276:immunology / Q000378:metabolism*; D009895:Opsonin Proteins; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D010587:Phagocytosis; D013481:Superoxides / Q000378:metabolism*; D013997:Time Factors
chemical_list: D006600:Hexosephosphates; D009895:Opsonin Proteins; D013481:Superoxides; D005947:Glucose