title: Simultaneous sampling of portal, hepatic and systemic blood during intragastric loading and tracer infusion in conscious pigs.
abstract: A surgical model for catheterization at multiple sites has been developed for use in long-term metabolic studies. For blood sampling, catheters were inserted into the portal and hepatic veins and the common carotid artery. The hepatic vein catheter was inserted from the margin of a liver lobe and led through the venous system, until the tip was close to the bifurcation with the inferior vena cava. A new technique was developed to ensure correct placement of the hepatic vein catheter using the specific extraction of indocyanin-green over the liver during surgery. Gastrostomy was performed using a Pezzer catheter. Catheters in the artery and hepatic and portal veins were patent for blood withdrawal for up to 4 weeks, and thus allowed repeated metabolic studies. Studies were performed in conscious animals familiar with the experimental situation.
authors.affiliation: Institute for Experimental Research in Surgery, University of Copenhagen, Denmark.
authors.forename: H P
authors.initials: HP
authors.lastname: Olesen
authors.forename: E
authors.initials: E
authors.lastname: Sjøntoft
authors.forename: B
authors.initials: B
authors.lastname: Tronier
mesh_terms: D000818:Animals; D001800:Blood Specimen Collection / Q000379:methods / Q000662:veterinary*; D002339:Carotid Arteries; D002405:Catheterization, Central Venous / Q000662:veterinary*; D004435:Eating; D005260:Female; D005774:Gastrostomy; D006503:Hepatic Veins; D008297:Male; D011169:Portal Vein; D013047:Specific Pathogen-Free Organisms; D013270:Stomach; D013552:Swine / Q000097:blood*