Properties of platelet concentrates prepared after extended whole blood holding time.
Extension of the maximum holding time for whole blood collected into a CPD-ADSOL system from 6 to 8 hours at ambient temperature under conditions that cause the temperature of the blood to decrease to 20 to 24 degrees C would facilitate the preparation of platelet concentrates (PCs). In this study, the properties of CPD-PCs prepared and stored for 5 days in PL-732 containers after various initial holding periods were assessed in two laboratories, designated Laboratory A and Laboratory B. Laboratory A found higher platelet-rich plasma (PRP) volumes (276 +/- 25 vs. 249 +/- 19 mL) and platelet yields (76 +/- 18 vs. 66 +/- 18 x 10(9) platelets) with 8-hour holds (n = 10) than with 1- to 2-hour holds (n = 10), although only the difference in PRP volumes was significant (p less than 0.05). No significant difference was observed in autologous in vivo recovery (54 +/- 11 vs. 47 +/- 9%) or survival (167 +/- 37 vs. 170 +/- 25 hrs), as calculated by gamma function using 111In as radiolabel. Laboratory B also found higher PRP volumes (304 +/- 31 vs. 279 +/- 37 mL) and platelet yields with 8-hour holds (n = 12) than with a 6-hour holds (n = 10) (88 +/- 26 vs. 77 +/- 27 x 10(9) platelets). No significant differences were found in morphology score, the extent of release of beta-thromboglobulin, the discharge of lactate dehydrogenase, or hypotonic shock response.(ABSTRACT TRUNCATED AT 250 WORDS)
Holme
S
S
American Red Cross and Eastern Virginia Medical School, Norfolk.
Moroff
G
G
Whitley
P
P
Hallinen
D
D
Heaton
A
A
D000255:Adenosine Triphosphate / Q000097:blood; D001792:Blood Platelets / Q000166:cytology / Q000502:physiology*; D001793:Blood Preservation; D001803:Blood Transfusion; D002470:Cell Survival; D006801:Humans; D007038:Hypotonic Solutions; D007770:L-Lactate Dehydrogenase / Q000097:blood; D017713:Platelet Transfusion; D011786:Quality Control; D013997:Time Factors; D001620:beta-Thromboglobulin / Q000378:metabolism
D007038:Hypotonic Solutions; D001620:beta-Thromboglobulin; D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase