[Investigations on methods of quantitative polarization optical estimation of neutral carbohydrates in extracellular matrix].
By means of the fluorescence PAS-reaction using a Schiff-type reagent substituted with acriflavine, the neutral carbohydrates were demonstrable selectively in the human trophoblast basement membrane. The reaction product is characterized by a typical birefringence that can be measured in the polarized light. The results obtained from ascertainment of the dispersion of the birefringence before and after the histochemical reaction, as well, gave further evidence for specificity and a definite improvement of the measurement of path differences.
Pfister
C
C
Institut für Anatomie, Bereich Medizin (Charité), Humboldt-Universität, Berlin, DDR.
Scheuner
G
G
Bahr
C
C
Hahn
C
C
D000167:Acriflavine; D001485:Basement Membrane / Q000166:cytology; D002241:Carbohydrates / Q000032:analysis*; D005109:Extracellular Matrix / Q000648:ultrastructure*; D005260:Female; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods; D011247:Pregnancy; D012545:Schiff Bases; D014327:Trophoblasts / Q000166:cytology
D002241:Carbohydrates; D012545:Schiff Bases; D000167:Acriflavine
Untersuchungen zur Methode der quantitativen polarisationsoptischen Erfassung neutraler Kohlenhydrate in der extracellulären Matrix.