The influence of nerve growth factor on the activities of adenylate cyclase and high-affinity GTPase in pheochromocytoma PC12 cell.
The influence of nerve growth factor (NGF) on the activities of adenylate cyclase and high-affinity GTPase in pheochromocytoma PC12 cells was studied. Incubation of cells with nerve growth factor led to a rapid activation of adenylate cyclase accompanied by an inhibition of high-affinity GTPase. By the 10th min of incubation the activity of adenylate cyclase had been reduced 2-fold when compared to the control. The activity of GTPase, however, increased. No significant changes in the cAMP level were detected. The data obtained indicate that NGF interaction with PC12 cells induces changes in the adenylate cyclase system and this process involves G-proteins that regulate the adenylate cyclase activity.
Golubeva
E E
EE
Institute of Applied Molecular Biology, USSR Ministry of Health, Moscow.
Posypanova
G A
GA
Kondratyev
A D
AD
Melnik
E I
EI
Severin
E S
ES
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D000242:Cyclic AMP / Q000378:metabolism; D004789:Enzyme Activation; D020558:GTP Phosphohydrolases / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000378:metabolism; D009414:Nerve Growth Factors / Q000494:pharmacology*; D010673:Pheochromocytoma / Q000201:enzymology*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D051381:Rats; D014407:Tumor Cells, Cultured
D009414:Nerve Growth Factors; D000246:Adenosine Diphosphate Ribose; D000242:Cyclic AMP; D010744:Phosphoric Monoester Hydrolases; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases