title: The amino acid sequence of low-potential cytochrome c550 from the cyanobacterium Microcystis aeruginosa.
abstract: The low-potential cytochrome c550 has been purified from the cyanobacterium Microcystis aeruginosa and its amino acid sequence has been determined. The protein contains 135 amino acid residues with the Cys-X-X-Cys-His heme binding site at residues 37 to 41. The sequence from residue 28 to 45 shows similarity to cytochrome c553 residues 1 to 18 when the heme binding sites are aligned. Another region of similarity is in the carboxyl-terminal regions of these two proteins. The two aligning regions of cytochrome c553 correspond to helical segments in other related cytochromes. A partial sequence of cytochrome c550 from Aphanizomenon flos-aquae was obtained and showed a 48% identity to the sequence of the M. aeruginosa cytochrome. The single methionine residue in cytochrome c550 of M. aeruginosa occurs at position 119 but there is no methionine in this region in the A. flos-aquae cytochrome, indicating that methionine is not the sixth ligand to the heme iron atom. Histidine 92 is a possible sixth ligand in M. aeruginosa cytochrome c550. The far-uv circular dichroism spectrum indicates that this protein is approximately 17% alpha helix, 42% beta-pleated sheet, and 41% random coil.
authors.affiliation: Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907.
authors.forename: C L
authors.initials: CL
authors.lastname: Cohn
authors.forename: J R
authors.initials: JR
authors.lastname: Sprinkle
authors.forename: J
authors.initials: J
authors.lastname: Alam
authors.forename: M
authors.initials: M
authors.lastname: Hermodson
authors.forename: T
authors.initials: T
authors.lastname: Meyer
authors.forename: D W
authors.initials: DW
authors.lastname: Krogmann
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D003488:Cyanogen Bromide; D003574:Cytochrome c Group / Q000302:isolation & purification*; D006868:Hydrolysis; D046931:Microcystis / Q000201:enzymology*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification; D012697:Serine Endopeptidases; D014357:Trypsin
chemical_list: D000596:Amino Acids; D003574:Cytochrome c Group; D010446:Peptide Fragments; C031732:cytochrome C-550; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase; D014357:Trypsin; D003488:Cyanogen Bromide