Recombinant soluble Fc epsilon receptor II (Fc epsilon RII/CD23) has IgE binding activity but no B cell growth promoting activity.
We have undertaken the production of recombinant soluble Fc epsilon receptor II (Fc epsilon RII) as a secretory protein, but not as a cleavage product of membrane-bound receptor. Several plasmid constructs containing soluble receptor sequence were prepared. Only a chimeric gene containing the sequences encoding IL-6 signal peptide and the soluble moiety of Fc epsilon RII could be expressed in Xenopus laevis oocytes and CHO cells, resulting in the secretion of soluble Fc epsilon RII. The recombinant soluble Fc epsilon RII was also produced in the yeast expression system. The NH2-terminal sequence analysis of the chimeric gene product generated by oocytes demonstrated the correct cleavage of IL-6 leader sequence by a signal peptidase. Moreover, most of CHO cell and all of the yeast-derived recombinant molecules were products identical with the native B cell-derived soluble Fc epsilon RII. These recombinant products as well as the natural soluble receptor derived from a human B cell line could bind both human IgE and two different anti-Fc epsilon RII mAb and could competitively inhibit the binding of IgE to Fc epsilon RII-expressing cells. However, the recombinant soluble Fc epsilon RII and highly purified native molecules did not display any B cell growth-promoting activity.
Uchibayashi
N
N
Institute for Molecular and Cellular Biology, Osaka University, Japan.
Kikutani
H
H
Barsumian
E L
EL
Hauptmann
R
R
Schneider
F J
FJ
Schwendenwein
R
R
Sommergruber
W
W
Spevak
W
W
Maurer-Fogy
I
I
Suemura
M
M
D000595:Amino Acid Sequence; D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D001402:B-Lymphocytes / Q000378:metabolism / Q000502:physiology*; D002454:Cell Differentiation / Q000187:drug effects; D006224:Cricetinae; D005822:Genetic Vectors; D006031:Glycosylation; D006801:Humans; D007073:Immunoglobulin E / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D015847:Interleukin-4; D007378:Interleukins / Q000235:genetics / Q000502:physiology*; D008222:Lymphokines / Q000235:genetics / Q000502:physiology*; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism; D029584:Prostatic Secretory Proteins; D011961:Receptors, Fc / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D017455:Receptors, IgE; D011994:Recombinant Proteins / Q000378:metabolism / Q000502:physiology; D012397:Rosette Formation; D012995:Solubility
D000944:Antigens, Differentiation, B-Lymphocyte; D007378:Interleukins; D008222:Lymphokines; D008565:Membrane Proteins; D029584:Prostatic Secretory Proteins; D011961:Receptors, Fc; D017455:Receptors, IgE; D011994:Recombinant Proteins; C043076:beta-microseminoprotein; C013059:immunoglobulin-binding factors; D015847:Interleukin-4; D007073:Immunoglobulin E