Antibodies to human immunodeficiency virus type 1 in the urine specimens of HIV-1-seropositive individuals.
Specific antibodies to human immunodeficiency virus type 1 (HIV-1) were detected in 200-fold concentrated urine samples, but none were detected in unconcentrated urine specimens, from 100 randomly selected HIV-1--seropositive individuals by enzyme-linked immunosorbent assay (ELISA) and Western blot techniques using the manufacturer's recommended procedures. Using modified methods for both the ELISA and Western blot tests, antibodies to HIV-1 have also been detected in the unconcentrated urine specimens from the same HIV-1--seropositive individuals. No difference in the frequency of antibodies to HIV-1 were found between unconcentrated and 200-fold concentrated urine samples when tested by the modified methods. HIV-1 core antigen (p24) was not detected in either the concentrated or the unconcentrated HIV-1--seropositive adult urine samples; none of these individuals showed overt clinical or laboratory evidence of renal dysfunction. The titer of the antibodies to HIV-1 found in the urine specimens was found to be parallel with the titer of antibodies to HIV-1 in the corresponding individual's serum. Further elucidation of the pathophysiology and the nature of the specific antibodies to HIV-1 observed in the urine of HIV-1--seropositive individuals is under investigation in our laboratories.
Cao
Y Z
YZ
New York University Medical Center, Department of Microbiology, NY 10016.
Hosein
B
B
Borkowsky
W
W
Mirabile
M
M
Baker
L
L
Baldwin
D
D
Poiesz
B J
BJ
Friedman-Kien
A E
AE
D000293:Adolescent; D000328:Adult; D000368:Aged; D000914:Antibodies, Viral / Q000652:urine; D015153:Blotting, Western; D003587:Cytomegalovirus / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015483:HIV Antibodies / Q000652:urine*; D006679:HIV Seropositivity / Q000276:immunology* / Q000652:urine; D015497:HIV-1 / Q000276:immunology*; D006510:Hepatitis B Antibodies / Q000652:urine; D006801:Humans; D008297:Male; D008875:Middle Aged
D000914:Antibodies, Viral; D015483:HIV Antibodies; D006510:Hepatitis B Antibodies