title: Comparison of amino acid sequence analysis by electron ionization and negative-ion chemical ionization mass spectrometry.
abstract: The positive electron ionization and negative chemical ionization mass spectra of 15 different derivatives of the tripeptide Phe-Ala-Leu have been compared. Total ion currents and ion currents of sequence-characterizing ions have been measured and compared. The negative-ion spectra, using 10% carbon dioxide in argon as moderator gas, proved to be simpler and contained more abundant sequence ions than the positive electron ionization spectra.
authors.affiliation: Institute of Organic Chemistry, University of Bern, Switzerland.
authors.forename: H
authors.initials: H
authors.lastname: Reinhard
authors.forename: R
authors.initials: R
authors.lastname: Lauber
authors.forename: U P
authors.initials: UP
authors.lastname: Schlunegger
mesh_terms: D000595:Amino Acid Sequence; D013058:Mass Spectrometry / Q000379:methods*