Trans-activation of herpes simplex virus type 1 immediate early genes is specifically inhibited by human recombinant interferons.
The effect of human recombinant interferons (IFNs) alpha and gamma on the transcription of Herpes Simplex virus type-1 (HSV-1) immediate early (IE) genes has been studied. Human amniotic cells (WISH) were transfected with a chimaeric plasmid containing the regulatory region of the HSV-1 IE genes controlling the expression of a reporter gene (CAT). Subsequently cells were infected with live HSV-1 to trans-activate the IE sequences. When cell samples were treated with IFN alpha or IFN gamma prior to viral infection, transcription of the CAT gene was greatly inhibited.
De Stasio
P R
PR
Department of Biology, Indiana University, Bloomington, IN 47405.
Taylor
M W
MW
D000650:Amnion; D015152:Blotting, Northern; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects*; D005814:Genes, Viral / Q000187:drug effects*; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology*; D009693:Nucleic Acid Hybridization; D011994:Recombinant Proteins; D018139:Simplexvirus / Q000187:drug effects / Q000235:genetics*; D014162:Transfection; D014775:Virus Activation / Q000187:drug effects
D007370:Interferon Type I; D011994:Recombinant Proteins; D007371:Interferon-gamma