title: Alteration of nuclear lamina protein in human fibroblasts infected with cytomegalovirus (HCMV).
abstract: Immunoblotting with monoclonal as well as polyclonal lamin antibodies revealed that nuclear lamina proteins of human foreskin fibroblasts (HFF) were differentially affected after infection with human cytomegalovirus (HCMV). Lamin A immunoreactivity was progressively lost during the course of the infectious cycle whereas that of lamin C was comparatively stable. This process was not observed in herpes simplex virus-infected HFF. On the other hand, noninfected arrested HFF stimulated by serum to enter S-phase also exhibited loss of lamin A immunoreactivity. Selective in vivo proteolysis of lamin A is suggested as the possible underlying mechanism.
authors.affiliation: Institut für Virologie, Universität Marburg, Federal Republic of Germany.
authors.forename: K
authors.initials: K
authors.lastname: Radsak
authors.forename: D
authors.initials: D
authors.lastname: Schneider
authors.forename: E
authors.initials: E
authors.lastname: Jost
authors.forename: K H
authors.initials: KH
authors.lastname: Brücher
mesh_terms: D002478:Cells, Cultured; D003586:Cytomegalovirus Infections / Q000378:metabolism* / Q000382:microbiology; D005347:Fibroblasts / Q000382:microbiology*; D006561:Herpes Simplex / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D015151:Immunoblotting; D007399:Interphase; D034904:Lamin Type A; D034882:Lamins; D009685:Nuclear Envelope / Q000382:microbiology; D009687:Nuclear Proteins / Q000378:metabolism*; D011233:Precipitin Tests
chemical_list: D034904:Lamin Type A; D034882:Lamins; D009687:Nuclear Proteins; C440308:lamin C