Genomic locus for a 140 kDa structural protein (pp150) of human cytomegalovirus in strains Towne and AD169.
An HCMV specific clone was isolated from a genomic library of human cytomegalovirus (HCMV) DNA cloned into the expression vector lambda gt11. This clone (lambda 111-1) expressed an HCMV/beta-galactosidase fusion protein which was reactive with rabbit antibody prepared against purified HCMV virions and dense bodies as well as human HCMV immune serum. By probing Western blots of HCMV virion proteins or HCMV-infected cells with antibody prepared against the fusion protein, the authentic gene product of clone lambda 111-1 was identified as a high molecular weight polypeptide of 140. Probing the restriction digests of HCMV DNA with insert DNA from the immunoreactive lambda gt11 clone permitted us to localize the coding sequence for the 140 kDa polypeptide to the long unique region (map coordinates of 0.16-0.18) on HCMV Towne and AD169 genomes.
Pande
H
H
Division of Immunology, Beckman Research Institute of the City of Hope, Duarte, California 91010.
Campo
K
K
Churchill
M A
MA
Zaia
J A
JA
D015139:Blotting, Southern; D015153:Blotting, Western; D003587:Cytomegalovirus / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D005796:Genes; D005814:Genes, Viral; D010750:Phosphoproteins / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics; D014763:Viral Matrix Proteins; D014764:Viral Proteins / Q000235:genetics*
D004279:DNA, Viral; D010750:Phosphoproteins; D011993:Recombinant Fusion Proteins; D014763:Viral Matrix Proteins; D014764:Viral Proteins; C056367:pp150 protein, Cytomegalovirus