Subtypes of intercalated cells in rat kidney collecting duct defined by antibodies against erythroid band 3 and renal vacuolar H+-ATPase.
The cellular distributions of the kidney form of the erythrocyte band 3 chloride/bicarbonate exchanger and the kidney vacuolar H+-transporting ATPase were examined in rat kidney collecting duct by immunocytochemical staining of adjacent semithin sections. Polyclonal anti-peptide antibodies directed against two regions of murine erythroid band 3 gave a pattern of basolateral labeling similar to that seen with antibodies directed against the entire protein. In the medullary collecting duct almost all intercalated cells expressed basolateral membrane band 3 and displayed apical membrane H+-ATPase. In the cortical collecting duct and the connecting segment, band 3 labeling was restricted to a subpopulation of intercalcated cells. In the cortical collecting duct 46% of intercalated cells had apical H+-ATPase and basolateral band 3. Cells that had either basolateral or diffuse cytoplasmic staining for H+-ATPase were all band 3-negative and accounted for 53% of the intercalated cells. In addition, occasional intercalated cells with apical H+-ATPase appeared to lack basolateral band 3. These results demonstrate the coexpression of H+-ATPase and band 3 in opposite plasma membrane domains of a subpopulation of intercalated cells that are probably the acid-excreting (type A) cells. All other intercalated cells lacked immunoreactive band 3 and probably include the bicarbonate-excreting (type B) cells.
Alper
S L
SL
Whitehead Institute, Cambridge, MA 02142.
Natale
J
J
Gluck
S
S
Lodish
H F
HF
Brown
D
D
D000818:Animals; D001457:Anion Exchange Protein 1, Erythrocyte / Q000032:analysis*; D000906:Antibodies; D000918:Antibody Specificity; D015153:Blotting, Western; D004910:Erythrocyte Membrane / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006801:Humans; D007672:Kidney Cortex / Q000166:cytology / Q000378:metabolism; D007684:Kidney Tubules / Q000166:cytology*; D007685:Kidney Tubules, Collecting / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D006180:Proton-Translocating ATPases / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D014617:Vacuoles / Q000201:enzymology / Q000648:ultrastructure*
D001457:Anion Exchange Protein 1, Erythrocyte; D000906:Antibodies; D006180:Proton-Translocating ATPases