Purification and characterisation of a growth factor from porcine bone.
A growth factor was extracted from porcine bone matrix by demineralisation and purified by heat and acid treatment, hydroxyapatite chromatography and gel filtration under dissociative conditions and reverse-phase HPLC. Using the mitogenic response of osteoblast-progenitor cells from embryonic chicken, a mitogenic activity was purified 3000-fold. The mitogenic protein thus purified shows an apparent molecular mass of 13.5 kDa in both the nonreduced and reduced form on sodium dodecyl sulfate/polyacrylamide gel electrophoresis. The mitogenic activity is sensitive to proteinase K, dithiothreitol, and resistant to DNAse, RNase, heat (70 degrees C) and pH (3-10). The factor stimulates the proliferation of osteoblast-progenitor cells from embryonic chick at a concentration of 1 ng/ml. It is active on cells from skin, periosteum and sternum and has no or little activity on cells of the calvaria, intestine or kidney of embryonic chick or on mouse AKR-2B/Balb c/3T3 cell line.
Mayer
H
H
Gesellschaft für Biotechnologische Forschung mbH, Department of Genetics, Braunschweig, Federal Republic of Germany.
Kukoschke
K G
KG
D000818:Animals; D001842:Bone and Bones / Q000032:analysis* / Q000502:physiology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D002845:Chromatography; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D017886:Durapatite; D006133:Growth Substances / Q000302:isolation & purification* / Q000494:pharmacology; D006882:Hydroxyapatites; D008970:Molecular Weight; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D013552:Swine
D006133:Growth Substances; D006882:Hydroxyapatites; D017886:Durapatite