Glucocorticoid administration increases receptor-mediated and forskolin-stimulated cyclic AMP accumulation in rat brain cerebral cortical slices.
Experiments were undertaken to examine the influence of dexamethasone administration on adrenergic receptor-mediated cAMP accumulation in rat brain cerebral cortical slices. While the initial response to continuous treatment with dexamethasone or ACTH was a decrease in the alpha-adrenergic receptor-mediated augmentation of isoproterenol-stimulated cAMP production, more prolonged administration of these substances also increased beta-adrenergic receptor-stimulated cAMP accumulation. Dose-response studies revealed a dose-dependent increase in beta-adrenergic receptor-stimulated cAMP production. Prolonged administration of either dexamethasone or ACTH also increased 2-chloradenosine-, vasoactive intestinal peptide (VIP)-, and forskolin-stimulated cAMP production in the brain slice. These results, together with previous findings, suggest that glucocorticoids have at least two effects on the brain neurotransmitter receptor-coupled cAMP generating system which may be important for maintaining homeostasis during times of stress.
Duman
R S
RS
Department of Pharmacology, University of Texas Medical School, Houston.
Strada
S J
SJ
Enna
S J
SJ
D015762:2-Chloroadenosine; D000241:Adenosine / Q000031:analogs & derivatives / Q000494:pharmacology; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D005576:Colforsin / Q000494:pharmacology*; D000242:Cyclic AMP / Q000378:metabolism*; D003907:Dexamethasone / Q000494:pharmacology*; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D017981:Receptors, Neurotransmitter / Q000187:drug effects / Q000502:physiology*; D012016:Reference Values; D012969:Sodium Fluoride / Q000494:pharmacology; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
D017981:Receptors, Neurotransmitter; D015762:2-Chloroadenosine; D005576:Colforsin; D014660:Vasoactive Intestinal Peptide; D003907:Dexamethasone; D012969:Sodium Fluoride; D000324:Adrenocorticotropic Hormone; D000242:Cyclic AMP; D000241:Adenosine; D007545:Isoproterenol