Positive identification of NS4A, the last of the hypothetical nonstructural proteins of flaviviruses.
A radiolabeled protein migrating in SDS-polyacrylamide gels near the core protein C of Kunjin virus-infected cells was isolated and subjected to N-terminal amino acid sequencing. Comparisons with the translation sequence deduced from the known nucleotide sequence identified a hydrophobic protein of 149 amino acids located in the polyprotein sequence between NS3 and NS4B, thus establishing its identity as NS4A with a calculated Mr 16,100. The cleavage sites identified at the N- and C-termini are KR decreases S....parallel....VAA decreases, both representing consensus sequences defined previously for Kunjin and other flaviviruses.
Speight
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Department of Microbiology, Monash University, Clayton, Victoria, Australia.
Westaway
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EG
D000595:Amino Acid Sequence; D000818:Animals; D002213:Capsid / Q000032:analysis*; D004279:DNA, Viral / Q000235:genetics; D005416:Flavivirus / Q000032:analysis* / Q000235:genetics; D005814:Genes, Viral; D008969:Molecular Sequence Data; D008970:Molecular Weight; D014709:Vero Cells; D014758:Viral Core Proteins / Q000032:analysis*; D017361:Viral Nonstructural Proteins
D004279:DNA, Viral; D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins