title: The effect of secretin on sodium ion absorption by the isolated human gallbladder.
abstract: Sodium ion (Na+) transport, a principal function of the gallbladder epithelium, was studied by measuring the flux of 22Na across isolated human gallbladder mucosa maintained in a modified 'Ussing' flux chamber. Tissue was obtained from cholecystectomy specimens in symptomatic patients with cholelithiasis. Out of 26 gallbladders studied, 13 had a net Na+ flux from mucosa to serosa which indicated active Na+ absorption. The hormone secretin, when added to the serosal fluid, reversed the direction of net flux in these gallbladders and caused a secretion of Na+ from serosa to mucosa. These results suggest that secretin may be involved in the physiological regulation of fluid transport in the human gallbladder, and also suggest a possible role for this hormone in gallbladder emptying.
authors.affiliation: Department of Medicine, Ninewells Hospital and Medical School, Dundee, Scotland, UK.
authors.forename: M R
authors.initials: MR
authors.lastname: Jacyna
authors.forename: P E
authors.initials: PE
authors.lastname: Ross
authors.forename: D
authors.initials: D
authors.lastname: Hopwood
authors.forename: I A
authors.initials: IA
authors.lastname: Bouchier
mesh_terms: D005704:Gallbladder / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D012633:Secretin / Q000494:pharmacology*; D012964:Sodium / Q000378:metabolism*; D012979:Sodium Radioisotopes
chemical_list: D012979:Sodium Radioisotopes; D012633:Secretin; D012964:Sodium