Transcriptional regulation of immunoglobulin heavy chain and T-cell receptor beta chain genes.
We have identified factors that bind to functionally important regions in IgH chain promoters and enhancer. One promoter factors is identical to u-EBP-E, an enhancer binding protein. Several promoter-binding proteins are present preferentially in either B cells or fibroblasts although most enhancer-binding proteins have a ubiquitous distribution. Additional characterization of these factors will further our understanding of the mechanisms by which IgH promoters and enhancers interact to achieve B-cell restricted and developmental stage-specific expression of IgH genes. The identification of a TCR beta chain enhancer will allow us to pursue similar questions with respect to the regulated expression this locus.
McDougall
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Laboratory of Biomedical and Environmental Science, UCLA 90024.
Eaton
S
S
Peterson
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CL
Calame
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K
D000818:Animals; D001483:Base Sequence; D015342:DNA Probes; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D016693:Receptors, Antigen, T-Cell, alpha-beta; D014157:Transcription Factors / Q000378:metabolism*
D015342:DNA Probes; D007143:Immunoglobulin Heavy Chains; D011948:Receptors, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta; D014157:Transcription Factors