[Immunohistochemical study of the expression of major histocompatibility complex (MHC)-determined antigens in renal tissue of MRL/lpr mice].
Expression of MHC antigens in renal tissue of MRL/lpr(H-2K) mice were demonstrated by monoclonal antibodies against class I(KK) and class II(IAK) antigens and ABC immunoperoxidase method. The expression of KK antigen in glomeruli, tubules and vessels of kidney and IAK antigen in glomeruli tubules was stronger in mice fed with beef tallow diet after gamma-interferon treatment than in the control group. This result suggests that using of gamma-interferon may enhance the presentation of MHC antigens in renal tissue of MRL/lpr mice. In mice raised with Menhaden fish oil diet after gamma-interferon treatment, however, the enhancement of expression of IAK antigen was detected only in renal tubules. In comparing with mice fed with beef tallow after gamma-interferon injection, the expression of KK antigen in glomeruli, vessels and IAK in the dendritic cells of renal interstitium was weaker in mice fed with fish oil. This result indicates that fish oil can more or less inhibit the expression of MHC antigens in renal tissue of mice. The mechanism of the inhibitory action of fish oil remains to be elucidated.
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D000818:Animals; D005395:Fish Oils / Q000627:therapeutic use; D015395:Histocompatibility Antigens Class I / Q000032:analysis*; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D007150:Immunohistochemistry; D007371:Interferon-gamma / Q000494:pharmacology; D007668:Kidney / Q000276:immunology*; D008181:Lupus Nephritis / Q000188:drug therapy / Q000235:genetics / Q000276:immunology*; D051379:Mice
D005395:Fish Oils; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D007371:Interferon-gamma