[Role of interleukin 2 and interferon gamma in induction of activated natural killer cells by the streptococcal preparation OK432].
The natural killer (NK) cell mediated cytotoxicity to syngeneic tumor cells can be augmented by in vivo priming and subsequent in vitro challenge with the streptococcal preparation OK432. Supernatants of coculture of spleen cells with OK432 contained Interleukin 2 (IL-2) and Interferon (IFN), mainly IFN-gamma. As the anti-mouse IFN-gamma monoclonal antibody but not anti-mouse IFN-alpha antibody inhibited the induction of activated NK cells with OK432, the IFN-gamma participated in this response. The incubated spleen cells with IL-2 receptors increased with OK432 treatment by flow cytometry, and the NK cell and IFN activities of supernatants were also abrogated by the treatment with anti-mouse IL-2 receptor monoclonal antibody to block the interaction between IL-2 and these receptors of effector cells. By panning method, it was clarified that the incubated spleen cells with IL-2 receptors were responsible for the production of IFN-r. These results suggest that IL-2 plays a major role to induce the activated NK cells from murine spleen cells primed in vivo and subsequently challenged in vitro with OK432, by the production of IFN-gamma.
Yamaue
H
H
Tanimura
H
H
Iwahashi
M
M
Tani
M
M
Tsunoda
T
T
Tabuse
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001688:Biological Products / Q000494:pharmacology*; D002478:Cells, Cultured; D005260:Female; D007371:Interferon-gamma / Q000494:pharmacology*; D007376:Interleukin-2 / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000187:drug effects*; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D010844:Picibanil / Q000494:pharmacology*
D000911:Antibodies, Monoclonal; D001688:Biological Products; D007376:Interleukin-2; D010844:Picibanil; D007371:Interferon-gamma