The effects of radical stress and N-ethylmaleimide on rat hepatic alpha 1-adrenergic receptors.
Liver plasma membranes of non-treated rats or rats treated with carbon tetrachloride were prepared. Liver plasma membranes obtained from non-treated rats were preincubated with cumene hydroperoxide, N-ethylmaleimide or hydrogen peroxide in the absence or presence of Fe2+. The effects of in-vivo or in-vitro treatment on hepatic alpha 1-adrenergic receptors were assessed in a [3H]prazosin binding assay. All treatments except preincubation with hydrogen peroxide alone caused a decrease in the number of alpha 1-adrenergic receptors without a change in the affinity of [3H]prazosin. The decrease in receptor number was accompanied by an increase in the level of lipid peroxidation in the plasma membranes. The results indicate that the hepatic alpha 1-adrenergic receptor is vulnerable to free radical stress.
Pruijn
F B
FB
Department of Pharmacochemistry, Faculty of Chemistry, Free University, Amsterdam, The Netherlands.
van Gelderen
J G
JG
Bast
A
A
D000818:Animals; D001555:Benzene Derivatives / Q000494:pharmacology; D002251:Carbon Tetrachloride / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D005033:Ethylmaleimide / Q000494:pharmacology*; D005609:Free Radicals; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008054:Lipid Peroxides / Q000096:biosynthesis; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D011224:Prazosin / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011942:Receptors, Adrenergic, alpha / Q000187:drug effects* / Q000378:metabolism
D001555:Benzene Derivatives; D005609:Free Radicals; D008054:Lipid Peroxides; D011942:Receptors, Adrenergic, alpha; D006861:Hydrogen Peroxide; D002251:Carbon Tetrachloride; D005033:Ethylmaleimide; C007164:cumene hydroperoxide; D011224:Prazosin