title: Restricted expression of viral glycoprotein in vesicular stomatitis virus-infected Drosophila melanogaster cells.
abstract: Vesicular stomatitis virus (VSV) establishes a non-cytopathic persistent infection in Drosophila melanogaster cells. The synthesis of the viral glycoprotein G was specifically inhibited during a post-transcriptional step, whereas the synthesis and turnover of its mRNA were not modified compared with the other viral mRNAs. Another viral glycoprotein, migrating slightly faster than G protein on an SDS-polyacrylamide gel, was detected in infected Drosophila cells. This protein showed most of the characteristics of the intracellular Gs protein found in infected vertebrate cells. The amounts of G protein integrated into mature virions and of soluble Gs protein secreted into the culture medium were reduced greatly during VSV infection in Drosophila cells.
authors.affiliation: Laboratoire de Génétique des Virus, CNRS, Gif sur Yvette, France.
authors.forename: F
authors.initials: F
authors.lastname: Wyers
authors.forename: D
authors.initials: D
authors.lastname: Blondel
authors.forename: A M
authors.initials: AM
authors.lastname: Petitjean
authors.forename: S
authors.initials: S
authors.lastname: Dezelee
mesh_terms: D000818:Animals; D001345:Autoradiography; D002460:Cell Line; D002642:Chick Embryo; D015342:DNA Probes; D004331:Drosophila melanogaster; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D008562:Membrane Glycoproteins; D009693:Nucleic Acid Hybridization; D011233:Precipitin Tests; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D014158:Transcription, Genetic; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics* / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D015342:DNA Probes; C028391:G protein, vesicular stomatitis virus; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D012367:RNA, Viral; D014759:Viral Envelope Proteins