Destruction of sympathetic and sensory neurons in the developing rat by a monoclonal antibody against the nerve growth factor (NGF) receptor.
The ability of the monoclonal antibody, 192-IgG, directed against the rat nerve growth factor (NGF) receptor to mimic or inhibit the actions of NGF was examined in vitro and in vivo. 192-IgG had no effect on morphology, survival, or protein synthesis rates of sympathetic neuronal cultures. When injected into newborn rats, destruction of sympathetic, but not sensory, neurons was produced. Injection prenatally produced more dramatic destruction of sympathetic neurons and, in addition, destruction of neural crest-derived sensory neurons. Therefore, although 192-IgG had no discernible effects in vitro, it produced a pattern of neuronal destruction in vivo qualitatively similar to that produced by antibodies to NGF itself.
Johnson
E M
EM
Department of Pharmacology, Washington University Medical School, St. Louis, MO 63110.
Osborne
P A
PA
Taniuchi
M
M
D000320:Adrenergic Fibers / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D009475:Neurons, Afferent / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D011956:Receptors, Cell Surface / Q000276:immunology / Q000502:physiology*; D017475:Receptors, Nerve Growth Factor
D000911:Antibodies, Monoclonal; D011956:Receptors, Cell Surface; D017475:Receptors, Nerve Growth Factor