An experimental collagen-impregnated Dacron graft: potential for endothelial seeding.
This study evaluates the potential for endothelial seeding of a collagen-impregnated Dacron graft with or without surface modifiers (fibronectin, heparin) to attach and retain these cells during flow. Human umbilical endothelial cells were harvested, cultured, labeled with Indium111-oxine and seeded onto 30 mm X 4 mm diameter grafts. Six graft surfaces were studied: 1) a collagen-impregnated Dacron graft, HemashieldR (C); 2) C + fibronectin (C + F); 3) C + heparin (C + H); 4) C + F + H; 5) HytrelR + F (Hyt + F); and 6) Hyt + F + H. Radioactive loss determined the percentage attachment and then percentage retention of labeled inoculum after a one-hour in vitro perfusion. Scanning electron and light microscopy demonstrated the endothelium on the graft surface following perfusion. Fibronectin-coated grafts had a significantly higher percentage attachment than those without fibronectin (ANOVA, P less than 0.05). However, the percentage retention following perfusion was similar for all Dacron grafts and statistically inferior to the HytrelR grafts studied (ANOVA, P less than 0.05). SEM evaluation of the C + F + H graft surface was qualitatively the most impressive Dacron surface for seeding, yet was inferior to the HytrelR graft. We conclude that fibronectin benefits the initial attachment of endothelium to collagen-coated Dacron rivaling the HytrelR surface. Fibronectin does not improve percentage retention of the HemashieldR surface during perfusion, therefore, some of its initial benefit is lost.
Dalsing
M C
MC
Indiana University Medical Center, Indianapolis 46202.
Kevorkian
M
M
Raper
B
B
Nixon
C
C
Lalka
S G
SG
Cikrit
D F
DF
Unthank
J L
JL
Herring
M B
MB
D001807:Blood Vessel Prosthesis; D002448:Cell Adhesion; D003094:Collagen / Q000008:administration & dosage; D004343:Drug Implants; D004730:Endothelium, Vascular / Q000166:cytology*; D005353:Fibronectins; D006493:Heparin; D006801:Humans; D011093:Polyethylene Terephthalates; D011474:Prosthesis Design
D004343:Drug Implants; D005353:Fibronectins; D011093:Polyethylene Terephthalates; D006493:Heparin; D003094:Collagen