title: [In vitro anti-HIV activity of phosphorothioate alpha-anomeric oligodeoxynucleotides].
abstract: Oligonucleotide analogs consisting exclusively of alpha-anomeric deoxynucleoside units bridged with phosphorothioate linkages have been synthesized and tested in vitro as antiviral agents against human immunodeficiency virus (HIV) in human T cells. Two 28-mers, an homopolymer alpha-S-dC28 and an oligomer alpha-S-anti-rev complementary to the initiation site of the regulatory viral gene rev exhibited antiviral activities comparable to those reported for the corresponding beta-anomeric phosphorothioate analogs. In contrast, a nuclease-resistant homopolymer, alpha-dC28 was inactive. Their preliminary results would indicate that the origin of oligonucleotide phosphorothioate anti-HIV activity is not exclusively correlated with their higher nuclease resistance.
authors.affiliation: Laboratoire de Chimie bio-organique, U.A. n 488, C.N.R.S., Université de Montpellier-II.
authors.forename: B
authors.initials: B
authors.lastname: Rayner
authors.forename: M
authors.initials: M
authors.lastname: Matsukura
authors.forename: F
authors.initials: F
authors.lastname: Morvan
authors.forename: J S
authors.initials: JS
authors.lastname: Cohen
authors.forename: J L
authors.initials: JL
authors.lastname: Imbach
mesh_terms: D001483:Base Sequence; D003864:Depression, Chemical; D006678:HIV / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D009841:Oligonucleotides / Q000494:pharmacology*; D016376:Oligonucleotides, Antisense; D013601:T-Lymphocytes / Q000276:immunology / Q000382:microbiology; D013873:Thionucleotides / Q000494:pharmacology*
chemical_list: D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; D013873:Thionucleotides
vernacular_title: Activité anti-VIH in vitro d'oligodésoxynucléotides phosphorothioates d'anomérie alpha.