Detection of latent virus mRNA in tissues using the polymerase chain reaction.
The polymerase chain reaction (PCR) has been adapted for use in gene expression studies. Using this technique, we have been able to specifically detect Herpes simplex virus gene expression in the amount of RNA equivalent to that present in a single mouse ganglion. We have also detected specific transcription of the ras oncogene in biopsies of hepatocellular carcinoma tissue. The single tube RNA PCR reaction should be readily adaptable for use as a rapid screening tool in virus diagnosis; it is capable of detecting several virus infections simultaneously using extremely small tissue biopsies.
Lynas
C
C
Department of Ophthalmology, University of Bristol, U.K.
Cook
S D
SD
Laycock
K A
KA
Bradfield
J W
JW
Maitland
N J
NJ
D000818:Animals; D004279:DNA, Viral / Q000032:analysis; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D005784:Gene Amplification; D005814:Genes, Viral; D011905:Genes, ras; D008113:Liver Neoplasms / Q000235:genetics; D051379:Mice; D012333:RNA, Messenger / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis*; D051381:Rats; D018139:Simplexvirus / Q000235:genetics*; D014158:Transcription, Genetic
D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral; D012321:DNA-Directed RNA Polymerases