title: Porin of Paramecium mitochondria isolation, characterization and ion selectivity of the closed state.
abstract: Porin was isolated and purified from mitochondria of Paramecium tetraurelia. The protein showed a single band of apparent Mr 37,000 on sodium dodecyl sulfate polyacrylamide electrophoretograms. The reconstitution of the protein into artificial lipid bilayer membranes revealed it to be a porin giving pores with an average single-channel conductance of 0.26 nS in 0.1 M KCl. This conductance is about half of that of other eukaryotic porins studied to date. The pore formed by the mitochondrial porin of Paramecium was found to be voltage-dependent and switched to a defined substrate at membrane voltages larger than 20 mV. In the open state the pore exhibited the characteristics of a general diffusion pore because the mobility sequence of the ions inside the pore was similar to that in the bulk aqueous phase. The effective diameter was estimated to be about 1.3 nm. The properties of the low conductance state of the pore were studied in detail. In this state the pore favored the passage of cations, in contrast to the open state which favored anions slightly. The possible role of the low-conductance state in the regulation of transport processes across the outer mitochondrial membrane and in mitochondrial metabolism is discussed.
authors.affiliation: Lehrstuhl für Biotechnologie, Universität Würzburg, F.R.G.
authors.forename: O
authors.initials: O
authors.lastname: Ludwig
authors.forename: R
authors.initials: R
authors.lastname: Benz
authors.forename: J E
authors.initials: JE
authors.lastname: Schultz
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000302:isolation & purification*; D002712:Chlorides / Q000378:metabolism; D008051:Lipid Bilayers; D008564:Membrane Potentials; D008928:Mitochondria / Q000032:analysis*; D008970:Molecular Weight; D010247:Paramecium / Q000648:ultrastructure*; D018272:Porins; D011188:Potassium / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; D002712:Chlorides; D008051:Lipid Bilayers; D018272:Porins; D011188:Potassium