A growth factor for hepatocytes is inactivated by sub-lethal gamma-irradiation in vivo or in vitro.
Serum from partially hepatectomized rats promoted DNA synthesis in primary adult rat hepatocyte cultures. If the rats had been exposed to sub-lethal gamma-irradiation immediately following operation or if their serum, collected at 3 h, was exposed to irradiation in vitro, the growth-promoting activity was destroyed. Prostaglandin E2 also stimulated DNA synthesis in the cultures; if PGE2 was irradiated in serum from intact or partially hepatectomized rats its growth-promoting activity was markedly diminished.
Skouteris
G G
GG
Department of Biochemistry, Oxford, England.
Ord
M G
MG
Stocken
L A
LA
D000818:Animals; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis*; D015232:Dinoprostone / Q000494:pharmacology / Q000528:radiation effects; D005720:Gamma Rays; D006133:Growth Substances / Q000494:pharmacology / Q000528:radiation effects*; D006498:Hepatectomy; D008099:Liver / Q000378:metabolism* / Q000528:radiation effects; D008115:Liver Regeneration; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D006133:Growth Substances; D004247:DNA; D000242:Cyclic AMP; D015232:Dinoprostone