title: Organization of mitochondrial UDP-glucose:dolichylmonophosphate glucosyltransferase in the outer membrane.
abstract: Previous studies have shown the existence of an autonomous mitochondrial UDP-glucose: dolichylmonophosphate glucosyltransferase, located in mitochondrial outer membrane of liver cells. To improve our knowledge about the topographical aspects of glycosylation in mitochondria, we have investigated the organization of this enzyme in intact mitochondria, using controlled proteolysis with trypsin and sensitivity towards amino-acid specific reagents. Our data provides evidence: --for a mitochondrial glucosyltransferase facing the cytoplasmic side of the outer membrane --and for the involvement of histidine and tryptophan residues as well as sulfhydryl groups in the catalytic activity of the enzyme.
authors.affiliation: University of Lyon, Lyon-Sud Medical School, Department of Biochemistry, INSERM U.189, CNRS.
authors.forename: F
authors.initials: F
authors.lastname: Gasnier
authors.forename: F
authors.initials: F
authors.lastname: Lerme
authors.forename: E
authors.initials: E
authors.lastname: Vaganay
authors.forename: P
authors.initials: P
authors.lastname: Louisot
authors.forename: O
authors.initials: O
authors.lastname: Gateau-Roesch
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D005964:Glucosyltransferases / Q000378:metabolism*; D007425:Intracellular Membranes / Q000201:enzymology; D051379:Mice; D008930:Mitochondria, Liver / Q000201:enzymology*; D014357:Trypsin
chemical_list: D000596:Amino Acids; D005964:Glucosyltransferases; C033917:UDPglucose dolicholphosphate glucosyltransferase; D014357:Trypsin