In vitro photosensitization II. An electron microscopy study of cellular destruction with mono-L-aspartyl chlorin e6 and photofrin II.
Primary sites of subcellular destruction with photofrin II (PfII) and mono-L-aspartyl chlorin e6 (MACE) were determined by transmission electron microscopy (TEM). Potorous tridactylus (PTK2) cells were grown in Rose chambers and incubated for 24 hr with a sensitizer concentration sufficient to provide 100% mortality. Cells were irradiated with laser light and fixed and processed for electron microscopy at various times post-irradiation. The results indicate that PfII initially destroys mitochondria, whereas MACE destroys lysosomes. Both conclusions are consistent with fluorescence subcellular localization studies.
Roberts
W G
WG
Department of Developmental Beckman Laser Institute and Medical Clinic, Irvine, CA 92715.
Liaw
L H
LH
Berns
M W
MW
D000818:Animals; D002478:Cells, Cultured; D017323:Dihematoporphyrin Ether; D006415:Hematoporphyrins / Q000494:pharmacology*; D066298:In Vitro Techniques; D007834:Lasers; D008247:Lysosomes / Q000187:drug effects; D007614:Macropodidae; D008854:Microscopy, Electron; D008928:Mitochondria / Q000187:drug effects; D010778:Photochemotherapy; D011166:Porphyrins / Q000494:pharmacology*; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*
D006415:Hematoporphyrins; D011166:Porphyrins; D011838:Radiation-Sensitizing Agents; D017323:Dihematoporphyrin Ether; C053434:Talaporfin