A monoclonal antibody to CD11c antigen inhibits the production of superoxide anion induced by concanavalin A in PMA-differentiated U-937 cells.
We have examined the effect of different monoclonal antibodies (MAb) to the CD11/CD18 family of surface antigens on the production of superoxide anion by phorbol myristate acetate (PMA)-differentiated U-937 cells upon stimulation with concanavalin A (Con A). The anti-CD11c MAb HC1/1 displayed a significant inhibition, whereas MAb to the other members of the CD11 family did not show a relevant inhibitory effect. These findings suggest that the CD11c antigen is involved in the triggering of the respiratory burst response by mononuclear phagocytic cells.
Cabañas
C
C
Centro de Investigaciones Biológicas, C.S.I.C., Madrid, Spain.
Lacal
P
P
Mollinedo
F
F
López-Rivas
A
A
Sánchez-Madrid
F
F
Bernabeu
C
C
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000378:metabolism*; D000954:Antigens, Surface; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D003208:Concanavalin A / Q000494:pharmacology*; D016167:Integrin alphaXbeta2; D013481:Superoxides / Q000096:biosynthesis*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D000954:Antigens, Surface; D016167:Integrin alphaXbeta2; D003208:Concanavalin A; D013481:Superoxides; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium