[In vitro anti-HIV activity of phosphorothioate alpha-anomeric oligodeoxynucleotides].
Oligonucleotide analogs consisting exclusively of alpha-anomeric deoxynucleoside units bridged with phosphorothioate linkages have been synthesized and tested in vitro as antiviral agents against human immunodeficiency virus (HIV) in human T cells. Two 28-mers, an homopolymer alpha-S-dC28 and an oligomer alpha-S-anti-rev complementary to the initiation site of the regulatory viral gene rev exhibited antiviral activities comparable to those reported for the corresponding beta-anomeric phosphorothioate analogs. In contrast, a nuclease-resistant homopolymer, alpha-dC28 was inactive. Their preliminary results would indicate that the origin of oligonucleotide phosphorothioate anti-HIV activity is not exclusively correlated with their higher nuclease resistance.
Rayner
B
B
Laboratoire de Chimie bio-organique, U.A. n 488, C.N.R.S., Université de Montpellier-II.
Matsukura
M
M
Morvan
F
F
Cohen
J S
JS
Imbach
J L
JL
D001483:Base Sequence; D003864:Depression, Chemical; D006678:HIV / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D009841:Oligonucleotides / Q000494:pharmacology*; D016376:Oligonucleotides, Antisense; D013601:T-Lymphocytes / Q000276:immunology / Q000382:microbiology; D013873:Thionucleotides / Q000494:pharmacology*
D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; D013873:Thionucleotides
Activité anti-VIH in vitro d'oligodésoxynucléotides phosphorothioates d'anomérie alpha.