Effect of a peptide derived from fibrinogen degraded by leukocyte elastase on isolated bovine mesenteric arteries.
A peptide derived from fibrinogen degraded by leukocyte elastase, and corresponding to amino acids 30-43 in the B beta-chain of fibrinogen, was evaluated concerning its effects on isolated bovine mesenteric arteries. This peptide induced dilation of the arteries and an increase in both cyclic AMP and cyclic GMP in the vessels. In addition there was an increase in 6-keto-PGF1 alpha indicating an increased release of prostacyclin. The increase in cyclic nucleotides and 6-keto-PGF1 alpha was inhibited by indomethacin, as was the vasodilation. The increase in cyclic GMP was much larger than the increase in cyclic AMP. The effects of the studied peptide are similar to the effects of other vasoactive peptides with a similar structure, such as bradykinin, neurotensin and substance P. The increase in cyclic AMP is probably caused by prostacyclin, a probable mediator of vasodilation. In addition, in certain species vasodilation may be caused by an increase in cyclic GMP.
Saldeen
K
K
Department of Pharmacology, Faculty of Health Sciences, Linköping, Sweden.
Andersson
R G
RG
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D000818:Animals; D002417:Cattle; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D005338:Fibrin Fibrinogen Degradation Products; D005340:Fibrinogen / Q000302:isolation & purification / Q000494:pharmacology*; D005345:Fibrinopeptide B / Q000302:isolation & purification / Q000494:pharmacology*; D066298:In Vitro Techniques; D007962:Leukocytes / Q000201:enzymology; D008638:Mesenteric Arteries / Q000187:drug effects* / Q000502:physiology; D010196:Pancreatic Elastase; D010446:Peptide Fragments / Q000302:isolation & purification / Q000494:pharmacology*; D014664:Vasodilation / Q000187:drug effects
D005338:Fibrin Fibrinogen Degradation Products; D010446:Peptide Fragments; C045012:fibrinogen Bbeta (30-43); D005345:Fibrinopeptide B; D015121:6-Ketoprostaglandin F1 alpha; D005340:Fibrinogen; D000242:Cyclic AMP; D010196:Pancreatic Elastase; D006152:Cyclic GMP