Bioelectrorheological model of the cell. 2. Analysis of creep and its experimental verification.
The electrorheological model of the cell proposed in Part 1 of this work was used to analyze changes in time of the shape of a cell acted on by a constant-amplitude external alternating electric field, with lossiness of the media taken into account. Shear stress in the cell membrane was determined. This model was then subjected to preliminary experimental verification using Neurospora crassa (slime) spheroplasts subjected to an external alternating electric field of constant frequency (3 MHz) and varying magnitude for different periods of time. Reversible viscoelastic cell deformation and fatigue (stiffening) of the material were observed. A satisfactory fit of the experimental data to Burgers' rheological model was found, and the values of the elastic moduli E1 = 9 X 10(2) N/m2, E2 = 2 X 10(2) N/m2 and viscosities eta 1 = 8 X 10(4) Ns/m2; eta 2 = 7 X 10(3) Ns/m2 were evaluated.
Fikus
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M
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
Pawlowski
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P
D002462:Cell Membrane / Q000502:physiology*; D008433:Mathematics; D008954:Models, Biological; D009492:Neurospora crassa; D012212:Rheology; D013314:Stress, Mechanical