Evidence for the existence of several different alpha- and beta-subunits of the GABA/benzodiazepine receptor complex from rat brain.
gamma-Aminobutyric acid (GABA)/benzodiazepine receptors purified from the brains of young and adult rats were photolabeled by [3H]flunitrazepam or [3H]muscimol. Gel electrophoresis revealed 3 different proteins with apparent molecular weight (Mr) 51,000, 53,000 and 59,000 which were specifically and irreversibly labeled by [3H]flunitrazepam and recognized by the alpha-subunit specific antibody bd-28. Similarly, 3 different proteins with Mrs 51,000, 53,000 and 56,000 were irreversibly labeled by [3H]muscimol and recognized by the beta-subunit-specific antibody bd-17. Comparison of the photolabeling and immunological staining pattern from young and adult rats seems to indicate that proteins labeled by [3H]flunitrazepam and bd-28 are different from those labeled by [3H]muscimol and bd-17.
Fuchs
K
K
Department of Biochemical Psychiatry, Psychiatrische Universitätsklinik, Vienna, Austria.
Sieghart
W
W
D000345:Affinity Labels; D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005445:Flunitrazepam; D007124:Immunoenzyme Techniques; D009118:Muscimol; D051381:Rats; D011963:Receptors, GABA-A / Q000032:analysis* / Q000378:metabolism
D000345:Affinity Labels; D011963:Receptors, GABA-A; D009118:Muscimol; D005445:Flunitrazepam