[Results of cross-species transplantation of substantia nigra to the lateral ventricle of the brain of rats with experimentally-induced Parkinson disease].
The method of compensation of dopamine (DA) deficit in the striatum by living, DA producing cells implanted into the brain, creates new hopes for patients with Parkinson's disease. In view of many ethical and legal problems, connected in some countries with obtaining human fetal tissue for transplantation, cross-species transplants would be an attractive alternative. The subject of our investigation was the problem of survival and functioning of embryonal substantia nigra (SN) neurons, taken from four species, implanted into the lateral ventricle of rats with denervated striatum. The recipients were 100 inbred rats of the Fisher 344 strain in which hemiparkinsonism was induced by unilateral chemical (6-OHDA) lesion of SN. As donor served foetuses of rabbits, hamsters, mouse CF1 and Sprague-Dawley rats. Immunosuppressive drugs were not applied. The effectiveness of the transplants was estimated by testing the rotational behaviour before and after transplantation over a period of 8 weeks at weekly intervals. Survival of the transplant was evaluated by histological and immunocytochemical methods. Behavioural tests demonstrated a statistically significant improvement persisting in groups of xenografts for three to five weeks. Histological investigations revealed that the time of graft survival corresponds roughly to the period of improvement in the rotational behaviour. The results indicate that without application of immunosuppressive drugs intracerebral transplant between different species survive no more than several weeks. Thus, it is necessary to search for homogenic material for therapeutic transplantations in parkinsonian patients.
Dymecki
J
J
Zakład Neuropatologii Instytutu Psychiatrii i Neurologii, Warszawa.
Freed
W J
WJ
D000818:Animals; D002552:Cerebral Ventricles / Q000601:surgery*; D004195:Disease Models, Animal; D006085:Graft Survival / Q000502:physiology; D006892:Hydroxydopamines; D051379:Mice; D016627:Oxidopamine; D010302:Parkinson Disease, Secondary / Q000139:chemically induced / Q000601:surgery*; D011817:Rabbits; D051381:Rats; D013378:Substantia Nigra / Q000637:transplantation*; D013997:Time Factors; D014183:Transplantation, Heterologous
D006892:Hydroxydopamines; D016627:Oxidopamine
Wyniki miedzygatunkowych transplantacji istoty czarnej do komory bocznej mózgu szczurów z doświadczalnie wywołanym parkinsonizmem.