title: Mouse hepatitis virus 3 replication in T and B lymphocytes correlate with viral pathogenicity.
abstract: Viral pathogenicity may be regulated by host defense mechanisms at the virus-immune cell interaction level. The immune system plays an important role in the outcome of acute disease induced by the mouse hepatitis virus type 3 (MHV3) virus. The lymphoid cells act as effectors in the virus elimination as well as targets for viral replication. In order to demonstrate a correlation between MHV3 pathogenicity and viral replication in lymphocytes, genetically-determined resistant A/J and susceptible C57BL/6 mice were infected with pathogenic (L2-MHV3) or nonpathogenic (YAC-MHV3) viral strains. Pathogenicity and histopathologic studies have revealed that lymphoid organs such as thymus and spleen, showed injuries or atrophy in susceptible mice infected with L2-MHV3. No histopathologic lesions in the lymphoid organs occurred in C57BL/6 mice infected with YAC-MHV3 or A/J mice infected with both viruses. The mechanisms involved in the lymphoid injuries were studied regarding viral replication in the lymphoid organs and cells in infected mice. Results indicate that cell depletion in lymphoid organs is caused by a complete viral replication in lymphoid cells. Thy1.2+ and surface IgM+ lymphoid cells from susceptible C57BL/6 mice infected with L2-MHV3 were permissive to viral replication and to subsequent cell lysis. No cell lysis, however, occurred in lymphoid cells from C57BL/6 mice infected with YAC-MHV3 and A/J mice infected with both virus strains. In vitro studies, with purified T and B cell populations were performed to determine the mechanism effecting susceptibility or resistance to viral-induced cell lysis occurring in such cells. A blockade, probably occurring at the viral RNA polymerase activity level, prevents viral replication in resistant cells between the stages of fixation of the virus at the cell-surface receptor and the viral protein translation. These experiments indicate that an intrinsic virus-specific resistant mechanism occurs in lymphoid cells that plays a major role in the viral pathogenicity.
authors.affiliation: Departement des Sciences Biologiques, Université du Québec à Montréal, Canada.
authors.forename: L
authors.initials: L
authors.lastname: Lamontagne
authors.forename: J P
authors.initials: JP
authors.lastname: Descoteaux
authors.forename: P
authors.initials: P
authors.lastname: Jolicoeur
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000382:microbiology* / Q000473:pathology; D004198:Disease Susceptibility; D006524:Hepatitis, Viral, Animal / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D007113:Immunity, Innate; D051379:Mice; D008805:Mice, Inbred A; D008810:Mice, Inbred C57BL; D006517:Murine hepatitis virus / Q000254:growth & development / Q000276:immunology / Q000472:pathogenicity*; D009928:Organ Specificity; D013154:Spleen / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology / Q000382:microbiology* / Q000473:pathology; D013950:Thymus Gland / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D014774:Virulence; D014779:Virus Replication