Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102.
Monoclonal antibodies against several human leucocyte cell surface antigens were prepared and characterized: (1) MEM-56, MEM-93, MEM-66, and MEM-104 against the CD45R antigen (220 kDa and 205 kDa mol. wt. forms of the leucocyte common antigen CD45 expressed on B-lymphocytes, myeloid cells and a subpopulation of T-lymphocytes); (2) MEM-98 and MEM-100 against the T-lymphocyte antigen CD6 (mol. wt. 100 kDa); (3) MEM-85 against the broadly expressed antigen CD44 (mol. wt. 85 kDa) which was recently shown by us to be identical with the lymphocyte homing receptor; (4) MEM-53 against a newly described broadly expressed antigen of 35-40 kDa mol. wt. and (5) MEM-102 against another newly described glycoprotein of 40 kDa mol. wt. anchored in membrane through a phosphatidylinositol moiety.
Bazil
V
V
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Praha.
Stefanová
I
I
Hilgert
I
I
Kristofová
H
H
Vanĕk
S
S
Bukovský
A
A
Horejsí
V
V
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology*; D000943:Antigens, Differentiation / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D006825:Hybridomas; D017493:Leukocyte Common Antigens; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D016201:Receptors, Lymphocyte Homing
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000943:Antigens, Differentiation; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D006023:Glycoproteins; D016201:Receptors, Lymphocyte Homing; D017493:Leukocyte Common Antigens