Class I MHC molecules and doxorubicin resistant P388 murine leukemic cells.
The major histocompatibility complex (MHC) encodes cell surface and secreted products involved in immune regulation and function. We have measured Class I MHC expression on doxorubicin (DOX) sensitive (P388/S) and resistant (P388/R84 and R84A) murine tumor cells using a monoclonal antibody to H-2d molecules. The present report shows a correlation between increased Class I MHC (H-2d) expression and drug resistance in P388 cells. Exposure of P388 cells to H-2d antibody diminished H-2d expression, whereas, treatment with murine recombinant gamma-interferon increased H-2d expression. Neither treatment significantly altered cellular DOX resistance or chemosensitivity. Thus, H-2d molecules can be used to identify DOX resistant P388 tumor cells but are probably not involved functionally in drug resistance.
DiCicco
L M
LM
Department of Oncology, University of Miami School of Medicine, Florida 33101.
Lazarus
H
H
Krishan
A
A
D000818:Animals; D004317:Doxorubicin / Q000494:pharmacology*; D004351:Drug Resistance; D006183:H-2 Antigens; D007371:Interferon-gamma / Q000494:pharmacology; D007941:Leukemia P388 / Q000188:drug therapy / Q000276:immunology*; D007942:Leukemia, Experimental / Q000276:immunology*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000276:immunology
D006183:H-2 Antigens; D004317:Doxorubicin; D007371:Interferon-gamma