Combined supercritical-fluid chromatography/mass spectrometry in the analysis of diuron in plasma using on-line phase-system switching.
On-line sample pretreatment by means of the phase-system switching approach is an interesting technique for the analysis of aqueous samples, e.g., plasma, by means of supercritical-fluid chromatography. In order to analyse plasma samples the following analytical procedure is used. The plasma sample is injected on to a short precolumn, which is washed with water and subsequently dried with nitrogen. Next, the solutes are desorbed with the supercritical mobile phase, analysed with packed-column supercritical-fluid chromatography and detected with either a UV detector or a mass spectrometer, equipped with a moving-belt interface. The herbicide diuron is selected as a test compound to study the feasibility of this approach. Using a selective detector the procedure is sufficiently sensitive to detect diuron in plasma, but not appropriate to detect the diuron metabolites in a post-mortem plasma sample. These have been identified with liquid chromatography/mass spectrometry. The detection limit of diuron in plasma using the procedure described is about 30 ng/mL.
Niessen
W M
WM
Division of Analytical Chemistry, Center for Bio-Pharmaceutical Sciences, Leiden, The Netherlands.
de Kraa
M A
MA
Verheij
E R
ER
Bergers
P J
PJ
La Vos
G F
GF
Tjaden
U R
UR
van der Greef
J
J
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004237:Diuron / Q000097:blood*; D006801:Humans; D066298:In Vitro Techniques; D013058:Mass Spectrometry / Q000379:methods
D004237:Diuron