Phosphorylation and dephosphorylation of the natriuretic peptide urodilatin (CDD-/ANP-95-126) and the effect on biological activity.
Urodilatin (CDD-/ANP-95-126), a new peptide hormone from human urine, is comprised of the same amino acid sequence as cardiodilatin (CDD-99-126/alpha-hANP) except for N-terminal extention by four amino acid residues. The presence of the recognition sequence Arg101-Arg-Ser-Ser104 for the cyclic AMP-dependent protein kinase enables rapid phosphorylation in the Ser104-position. Phosphorylation of urodilatin is associated with decreased vasorelaxant potency, while dephosphorylation of "phospho-urodilatin" by acidic phosphatase completely restores bioactivity.
Dörner
T
T
Institut für Anatomie und Zellbiologie III, Universität Heidelberg, FRG.
Gagelmann
M
M
Feller
S
S
Herbst
F
F
Forssmann
W G
WG
D000135:Acid Phosphatase; D000595:Amino Acid Sequence; D000818:Animals; D001011:Aorta / Q000187:drug effects; D009320:Atrial Natriuretic Factor / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D010446:Peptide Fragments / Q000378:metabolism*; D010766:Phosphorylation; D011817:Rabbits; D014665:Vasodilator Agents
D007202:Indicators and Reagents; D010446:Peptide Fragments; D014665:Vasodilator Agents; C056452:Ularitide; D009320:Atrial Natriuretic Factor; D000135:Acid Phosphatase