[Effects of different buffers, ethanol and hydrogen peroxide on the morphology of cultured brain cells].
Effects of different buffers: N-2-Hydroxyethylpiperazine-N-2-ethanesulfonic acid (HEPES), Ethanolamine (2-Aminoethanol), sodium bicarbonate (NaHCO3) in the presence of 0.003% H2O2 or 0.5% ethanol were investigated on cell cultures from brain (chick embryo 5 1/2 or 9 day in ovo). Morphological parameters: density of cells, reaggregation, size of cell aggregates were used. H2O2 in the presence of NaHCO3 led to a decrease of isolated cells and an increase of reaggregation with degeneration in the great clusters. When H2O2 and 5 mM HEPES were present together in the absence of NaHCO3, the aggregation was less than with HEPES alone. By use of a chemical defined medium DME/F-12 supplemented with Insulin, Transferrin, sodium selenite, bovine serum albumin and 15 mM HEPES both the number and the diameter of cell aggregates increased. Addition of 0.5% ethanol in the presence of NaHCO3 stimulated in cultures of the ganglion trigeminale and telencephalon (9 day chick embryos) the regeneration of neurites and the proliferation and migration of non-neural cells. No growth of neurites was observed on the same condition in brain cultures from 6 day chick embryo. The number of large aggregates increased significantly in the presence of NaHCO3 and in the chemical defined and supplemented medium DME/F-12. Bologa et al. (1987) demonstrated that the buffers TREA (Triethanolamine) and HEPES enhance the expression of properties related to post-mitotic differentiated cellular states in both neurons and glia.(ABSTRACT TRUNCATED AT 250 WORDS)
Lindner
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G
Institut für Anatomie des Bereiches Medizin (Charité), Humboldt-Universität, Berlin.
Grosse
G
G
Wenzel
M
M
D000818:Animals; D001639:Bicarbonates / Q000494:pharmacology*; D001921:Brain / Q000166:cytology* / Q000187:drug effects; D002449:Cell Aggregation / Q000187:drug effects; D002452:Cell Count; D002478:Cells, Cultured; D002642:Chick Embryo; D046508:Culture Techniques / Q000379:methods*; D000431:Ethanol / Q000494:pharmacology*; D006531:HEPES / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D010879:Piperazines / Q000494:pharmacology*; D012964:Sodium / Q000494:pharmacology*; D017693:Sodium Bicarbonate; D012668:Trigeminal Ganglion / Q000166:cytology / Q000187:drug effects
D001639:Bicarbonates; D010879:Piperazines; D000431:Ethanol; D017693:Sodium Bicarbonate; D012964:Sodium; D006861:Hydrogen Peroxide; D006531:HEPES
Wirkung von Puffern, Aethanol und H2O2 auf die Morphologie kultivierter ZNS-Zellen.