Enkephalins and exorphins oxidation by tyrosinase.
Tyrosinase activity was tested on some tyrosine-containing peptides (enkephalins and exorphins). All they are substrates for tyrosinase, showing a good affinity for the enzyme, in some cases higher than tyrosine itself. Aminoacid analysis after hydrolysis of long-lasting incubation mixtures of tyrosinase with Leu-enkephalin in presence of reductants demonstrates the formation of DOPA. The production of a new peptide containing DOPA derived from the oxidation of Leu-enkephalin was revealed by high performance liquid chromatography (HPLC).
Rosei
M A
MA
Dipartimento di Scienze Biochimiche, Università La Sapienza, Roma.
Antonilli
L
L
Coccia
R
R
Foppoli
C
C
D000595:Amino Acid Sequence; D004156:Catechol Oxidase / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004295:Dihydroxyphenylalanine / Q000032:analysis; D004743:Enkephalin, Leucine / Q000378:metabolism; D004745:Enkephalins / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D014442:Monophenol Monooxygenase / Q000378:metabolism*; D010084:Oxidation-Reduction; D010455:Peptides / Q000378:metabolism*
D004745:Enkephalins; D010455:Peptides; D004743:Enkephalin, Leucine; D004295:Dihydroxyphenylalanine; C019178:exorphins; D004156:Catechol Oxidase; D014442:Monophenol Monooxygenase