Characterization of cellular receptors for erythroid differentiation factor on murine erythroleukemia cells.
Erythroid differentiation factor (EDF), which is structurally related to transforming growth factor-beta family and induces differentiation of murine erythroleukemia cell clone F5-5, has been labeled with 125I to characterize its interaction with cellular receptors. Binding of 125I-EDF to F5-5 cells is time- and temperature-dependent, specific, saturable, and reversible. Transforming growth factor-beta 1 has no significant effects on growth of F5-5 cells and binding of 125I-EDF to F5-5 cells. Scatchard analysis of the binding data indicated that F5-5 cells have a single class of binding sites (3,200/cell) with an apparent Kd of 3.1 X 10(-10) M. Affinity cross-linking experiments demonstrated three radiolabeled components of 140,000, 76,000, and 67,000 daltons under both reducing and nonreducing conditions. Labeling of these three components has been inhibited by incubation of the cells with excess unlabeled EDF. These results imply molecular weights of 115,000, 51,000, and 42,000 for the EDF receptors on this cell line.
Hino
M
M
Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Tojo
A
A
Miyazono
K
K
Miura
Y
Y
Chiba
S
S
Eto
Y
Y
Shibai
H
H
Takaku
F
F
D028341:Activins; D000818:Animals; D001667:Binding, Competitive; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D007265:Inhibins / Q000378:metabolism* / Q000494:pharmacology; D007700:Kinetics; D004915:Leukemia, Erythroblastic, Acute; D051379:Mice; D008970:Molecular Weight; D011956:Receptors, Cell Surface / Q000302:isolation & purification / Q000502:physiology*; D017978:Receptors, Growth Factor; D011994:Recombinant Proteins / Q000494:pharmacology
D006133:Growth Substances; D011956:Receptors, Cell Surface; D017978:Receptors, Growth Factor; D011994:Recombinant Proteins; C060000:erythroid differentiation factor receptor; D028341:Activins; D007265:Inhibins