Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda.
A novel bacteriophage lambda vector system was used to express in Escherichia coli a combinatorial library of Fab fragments of the mouse antibody repertoire. The system allows rapid and easy identification of monoclonal Fab fragments in a form suitable for genetic manipulation. It was possible to generate, in 2 weeks, large numbers of monoclonal Fab fragments against a transition state analog hapten. The methods described may supersede present-day hybridoma technology and facilitate the production of catalytic and other antibodies.
Huse
W D
WD
Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037.
Sastry
L
L
Iverson
S A
SA
Kang
A S
AS
Alting-Mees
M
M
Burton
D R
DR
Benkovic
S J
SJ
Lerner
R A
RA
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000235:genetics; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D010582:Bacteriophage lambda / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004926:Escherichia coli / Q000235:genetics; D005784:Gene Amplification; D015723:Gene Library; D005822:Genetic Vectors; D006433:Hemocyanins / Q000031:analogs & derivatives / Q000276:immunology; D007140:Immunoglobulin Fab Fragments / Q000096:biosynthesis; D007128:Immunoglobulin Fragments / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D009943:Organophosphorus Compounds / Q000276:immunology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments; D007128:Immunoglobulin Fragments; D009943:Organophosphorus Compounds; D011994:Recombinant Proteins; C056997:keyhole limpet hemocyanin phosphonamidate conjugate; D006433:Hemocyanins