Purification of a functional 40 kD human placental Fc gamma-receptor using a monoclonal antibody.
F(ab')2-fragments of a mouse monoclonal antibody (B1D6) reacting with placental receptors for the Fc part of IgG (FcR) were used as affinity reagents for the purification of an antigen from placental extract (PE). The antigen agglutinated ovine erythrocytes (E) sensitized with rabbit antibodies (A) (EA), but not E or E sensitized with F(ab')2-fragments. It reduced the EA rosette-formation with mononuclear cells and the binding of soluble immune complexes to placental tissue. The antigen bound to aggregated IgG and Fc-fragments of IgG, but not to native IgG or F(ab')2-fragments of IgG. The data indicate that the purified antigen possesses FcR activity with low affinity for IgG. SDS-PAGE and Western blot showed one distinct band of approximately 40 kD. The electrophoretic mobility did not change after reduction and the band reacted with concanavalin A indicating that the FcR are single-chained glycoproteins.
Matre
R
R
Broegelmann Research Laboratory for Microbiology, Gade Institute, University of Bergen, Norway.
Kristoffersen
E K
EK
Ulvestad
E
E
Vedeler
C A
CA
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000302:isolation & purification*; D015153:Blotting, Western; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007074:Immunoglobulin G; D007158:Immunologic Techniques; D011257:Pregnancy Proteins / Q000302:isolation & purification; D011961:Receptors, Fc / Q000302:isolation & purification*; D017452:Receptors, IgG
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D007074:Immunoglobulin G; D011257:Pregnancy Proteins; D011961:Receptors, Fc; D017452:Receptors, IgG