A conformational immunogen on VP-2 of infectious bursal disease virus that induces virus-neutralizing antibodies that passively protect chickens.
VP-2b, a major structural protein of infectious bursal disease virus (IBDV), and its precursor protein VP-2a were separated in a soluble form from the supernatant of ultracentrifuged viruses by using a monoclonal antibody specific for VP-3, the other major structural protein, to remove soluble VP-3 and remaining virus particles. The native VP-2a/2b inhibited the majority of virus-neutralizing (VN) activity in chicken anti-IBDV sera and chickens immunized with VP-2a/2b produced VN antibodies that passively protected susceptible chickens from infection. However, the separated VP2a/2b was not as immunogenic as intact virus particles. VP-2a/2b would appear to contain a conformational epitope which is destroyed by SDS and boiling and which may prove to be of critical importance in a subunit vaccine against type 1 IBDV.
Fahey
K J
KJ
CSIRO Division of Animal Health, Animal Health Research Laboratory, Parkville, Victoria, Australia.
Erny
K
K
Crooks
J
J
D000327:Adsorption; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology* / Q000302:isolation & purification; D002645:Chickens / Q000276:immunology; D007116:Immunization, Passive; D007243:Infectious bursal disease virus / Q000276:immunology* / Q000302:isolation & purification; D009500:Neutralization Tests; D011201:Poultry Diseases / Q000517:prevention & control; D011487:Protein Conformation; D012087:Reoviridae / Q000276:immunology*; D012088:Reoviridae Infections / Q000517:prevention & control / Q000662:veterinary; D014764:Viral Proteins / Q000276:immunology* / Q000302:isolation & purification; D015678:Viral Structural Proteins
D000914:Antibodies, Viral; D000956:Antigens, Viral; D014764:Viral Proteins; D015678:Viral Structural Proteins