Enhanced protein phosphorylation, IL-2 receptor expression, and IL-2 production precede cellular proliferation in lymphocytes oxidized by periodic acid.
Increased rates of protein phosphorylation, IL-2 receptor (IL-2R) expression, IL-2 production and DNA synthesis were quantified in rat lymphocytes oxidized by periodic acid (H5IO6). Enhanced phosphorylation of 97, 59 and 37 to 29 kDa proteins in lymphocytes was detected at 18 and 36 hrs post-oxidation, whereas IL-2R expression and IL-2 elaboration were at their maximum by 24 hrs. The number of oxidized cells entering the S-phase of the cell cycle and their thymidine incorporation rates reached their maximum at 72 hrs. These results indicate that H5IO6-induced blastogenesis elicits a progression of responses temporally similar to those of lymphocytes stimulated by lectins.
Malinin
G I
GI
Department of Physics, Georgetown University, Washington, DC 20057.
Hornicek
F J
FJ
Malinin
T I
TI
D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000096:biosynthesis*; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects*; D010084:Oxidation-Reduction; D010504:Periodic Acid / Q000494:pharmacology*; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats; D015375:Receptors, Interleukin-2 / Q000378:metabolism*; D013997:Time Factors
D007376:Interleukin-2; D010750:Phosphoproteins; D015375:Receptors, Interleukin-2; D010504:Periodic Acid