Influence of PAF-acether on lecithin-oriented multibilayers monitored by ESR: interaction of PAF-acether with cholesterol.
The influence of PAF-acether on natural ovolecithin oriented multibilayers is detected by ESR of intercalated 5 doxyl stearic acid. Simulation of lineshapes demonstrates an enlarged orientational distribution of the local director of the phospholipid phase and a small increase of the order parameter. The amount of PAF-acether required to destabilize the ovolecithin lamellar phase depends on the degree of hydration. By contrast, cholesterol displays an organizing effect on the PAF-acether phase. Simulation establishes a sharp orientational distribution of the local director when cholesterol reaches stoichiometric ratio relative to PAF-acether. At the same time, cholesterol increases the order parameter.
Olivier
J L
JL
URA CNRS 217, CHU St. Antoine, Paris, France.
Chachaty
C
C
Wolf
C
C
Bereziat
G
G
D002784:Cholesterol / Q000494:pharmacology*; D004578:Electron Spin Resonance Spectroscopy; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D010972:Platelet Activating Factor / Q000031:analogs & derivatives* / Q000494:pharmacology; D013113:Spin Labels
D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D010972:Platelet Activating Factor; D013113:Spin Labels; C037145:1-hexadecyl-2-acetyl-glycero-3-phosphocholine; C026613:1-0-octadecyl 2-0-acetyl sn-glycero-3-phosphorylcholine; D002784:Cholesterol