title: Polyphosphate-degrading enzymes in Acinetobacter spp. and activated sludge.
abstract: Polyphosphate-degrading enzymes were studied in Acinetobacter spp. and activated sludge. Polyphosphate: AMP phosphotransferase activity in Acinetobacter strain 210A decreased with increasing growth rates. The activity of this enzyme in cell extracts of Acinetobacter strain 210A was maximal at a pH of 8.5 and a temperature of 40 degrees C and was stimulated by (NH4)2SO4. The Km for AMP was 0.6 mM, and the Vmax was 60 nmol/min per mg of protein. Cell extracts of this strain also contained polyphosphatase, which was able to degrade native polyphosphate and synthetic magnesium polyphosphate and was strongly stimulated by 300 to 400 mM NH4Cl. A positive correlation was found between polyphosphate:AMP phosphotransferase activity, adenylate kinase activity, and phosphorus accumulation in six Acinetobacter strains. Significant activities of polyphosphate kinase were detected only in strain P, which contained no polyphosphate:AMP phosphotransferase. In samples of activated sludge from different plants, the activity of adenylate kinase correlated well with the ability of the sludge to remove phosphate biologically from wastewater.
authors.affiliation: Department of Microbiology, Wageningen Agricultural University, The Netherlands.
authors.forename: J W
authors.initials: JW
authors.lastname: van Groenestijn
authors.forename: M M
authors.initials: MM
authors.lastname: Bentvelsen
authors.forename: M H
authors.initials: MH
authors.lastname: Deinema
authors.forename: A J
authors.initials: AJ
authors.lastname: Zehnder
mesh_terms: D017766:Acid Anhydride Hydrolases; D000150:Acinetobacter / Q000201:enzymology* / Q000254:growth & development; D000263:Adenylate Kinase / Q000032:analysis; D001673:Biodegradation, Environmental; D006863:Hydrogen-Ion Concentration; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis; D010770:Phosphotransferases / Q000032:analysis; D017856:Phosphotransferases (Phosphate Group Acceptor); D011122:Polyphosphates / Q000378:metabolism*; D012722:Sewage; D013696:Temperature; D014865:Waste Disposal, Fluid; D014871:Water Microbiology
chemical_list: D011122:Polyphosphates; D012722:Sewage; D010770:Phosphotransferases; D017856:Phosphotransferases (Phosphate Group Acceptor); C059169:polyphosphate AMP phosphotransferase; C022424:polyphosphate kinase; D000263:Adenylate Kinase; D010744:Phosphoric Monoester Hydrolases; D017766:Acid Anhydride Hydrolases; C022563:endopolyphosphatase