An enzyme-linked immunosorbent assay for detection of antibodies to porcine parvovirus.
An enzyme-linked immunosorbent assay (ELISA) was developed for detection of antibodies to porcine parvovirus (PPV). Antisera to PPV were raised in pigs, for which PPV grown on PK15 cells was used for primary intranasal inoculations, and PPV cultured on autologous kidney cells for booster immunisations. A competition ELISA was developed, based on the principle of a double antibody sandwich assay, using immunoglobulin fractions prepared from these sera. The ELISA was compared with a haemagglutination inhibition (HI) test. The tests were equally sensitive for detecting antibodies early after infection and for detecting a significant increase in antibody titre between paired sera. A high correlation was found between antibody titres of field sera measured by the two tests (r = 0.91). We conclude that ELISA is preferable to the HI test, because it is labour-saving and can be standardised better and automated.
Westenbrink
F
F
Centraal Diergeneeskundig Instituut, Lelystad, The Netherlands.
Veldhuis
M A
MA
Brinkhof
J M
JM
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005189:False Positive Reactions; D006385:Hemagglutination Inhibition Tests; D007106:Immune Sera / Q000276:immunology; D010321:Parvoviridae / Q000276:immunology*; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D011237:Predictive Value of Tests; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*
D000914:Antibodies, Viral; D007106:Immune Sera