[Studies on the serological activity of ND-O-BSA and its application in serodiagnosis of leprosy].
In this article, optimum conditions of ND-O-BSA-ELISA were determined and comparisons made of the serologic activity of 3 different types of antigens (WML, PGI, ND-O-BSA) in leprosy (151) and tuberculosis (20) patients and normal controls (42). The results indicated that: 1) optimum conditions for ND-O-BSA-ELISA were ND-O-BSA and OPD concentration of 0.1 and 400 micrograms/ml respectively with HRP-IgM and test sera dilutions of 1:1000 and 1:200; and 2) serological activity showed highly significant positive correlations between 3 types of antigens (WML vs PGI: r = 0.88, P less than 0.0005; PGI vs ND-O-BSA: r = 0.86, P less than 0.0005), all of which were highly sensitive and specific for leprosy serodiagnosis with positive rates of 100% (PGI) and 97.4% (WML, ND-O-BSA) in leprosy patients. None were positive in normal controls, but crossreactivity of different degree were present in tuberculosis patients. The authors suggest that PGI-ELISA and ND-O-BSA-ELISA be used in combination in the evaluation of clinical therapeutic efficacy and determination of disease stages, as well as in epidemiologic survey of subclinical leprosy infection in "high-risk" areas.
Wu
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D000942:Antigens, Bacterial / Q000032:analysis; D004187:Disaccharides; D004797:Enzyme-Linked Immunosorbent Assay; D006017:Glycolipids / Q000032:analysis*; D006801:Humans; D007918:Leprosy / Q000175:diagnosis*; D009166:Mycobacterium leprae; D012710:Serum Albumin, Bovine / Q000032:analysis*; D014376:Tuberculosis / Q000175:diagnosis
D000942:Antigens, Bacterial; D004187:Disaccharides; D006017:Glycolipids; C051559:O-(3,6-di-O-methylglucopyranosyl)-(1-4)-O-(2,3-di-O-methylrhamnopyranosyl)-(1-9)-oxynonanoyl-bovine serum albumin; C045169:phenolic glycolipid I, Mycobacterium leprae; D012710:Serum Albumin, Bovine