Rapid release of multiple hormones from rat pituitaries perifused with recombinant interleukin-1.
Previous studies demonstrated a direct action of interleukin-1 (IL-1) on release of hormones from rat anterior pituitary cells in monolayer culture. To rule out any possibility of a paracrine effect from the elevated hormones in the static monolayer system, and to examine further the dynamics of hormone release elicited by IL-1, studies were conducted with rat anterior pituitary tissue in a computer-controlled automated perifusion system. In experiments performed on the same day as sacrifice, IL-1 stimulated the release of adrenocorticotrophic hormone (ACTH), luteinizing hormone (LH), thyroid stimulating hormone (TSH), growth hormone (GH) and prolactin (PRL) in a dose-related manner. Peak levels were achieved within 6 minutes of exposure to IL-1. However, PRL was not increased over the baseline fluctuations when pituitaries were perifused with IL-1 after 72 hours of incubation. Hormone release did not appear to undergo desensitization after multiple short pulses of IL-1. Heat-denatured IL-1 had no effect on hormone release. The rapid response suggests that IL-1 acts acutely to release preformed hormone stores.
Beach
J E
JE
Division of Medicine, Walter Reed Army Institute of Research, Washington, D.C. 20307-5100.
Smallridge
R C
RC
Kinzer
C A
CA
Bernton
E W
EW
Holaday
J W
JW
Fein
H G
HG
D000818:Animals; D000242:Cyclic AMP / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000502:physiology*; D010477:Perfusion; D010903:Pituitary Gland, Anterior / Q000166:cytology / Q000378:metabolism*; D010907:Pituitary Hormones / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011994:Recombinant Proteins / Q000494:pharmacology; D013997:Time Factors
D007375:Interleukin-1; D010907:Pituitary Hormones; D011994:Recombinant Proteins; D000242:Cyclic AMP