title: Rapid detection of varicella-zoster virus in clinical specimens using monoclonal antibodies on shell vials and smears.
abstract: Monoclonal antibodies were used for detecting varicella-zoster virus (VZV) by immunofluorescence (IF) in clinical specimens inoculated on shell vial cultures. Vesicles of 74 patients with varicella or herpes zoster-like eruptions were tested by this method and by conventional cell culture. Further diagnostic tests were direct IF on smears (42 patients), the cytological Tzanck test (28 patients), and serology (30 patients). Both IF assays were performed with the commercial VZV-specific monoclonal antibody 3B3 (Ortho). Using the shell vial technique, we found VZV in 37 (50%) of the patients, on average after 3 days. The conventional culture method yielded 26 positives (35%), on average after 7.5 days. Twenty-nine of the shell vial IF-positive patients were also positive by direct IF on smears (30 tested), while 15 gave positive Tzanck smears (18 tested). The sensitivities of the shell vial IF test, the direct IF test, the conventional culture method, and the Tzanck test were about 95%, 97%, 70%, and 79%, respectively. For the specificities, we found at least 97% for the shell vial IF test, at least 91% for the direct IF test, and about 78% for the Tzanck test. We conclude that both VZV IF tests are much more reliable than the conventional cell culture method and the Tzanck test for the laboratory diagnosis of VZV infections.
authors.affiliation: Regional Public Health Laboratory, University Hospital, Groningen, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: Schirm
authors.forename: J J
authors.initials: JJ
authors.lastname: Meulenberg
authors.forename: G W
authors.initials: GW
authors.lastname: Pastoor
authors.forename: P C
authors.initials: PC
authors.lastname: van Voorst Vader
authors.forename: F P
authors.initials: FP
authors.lastname: Schröder
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D005455:Fluorescent Antibody Technique; D006562:Herpes Zoster / Q000175:diagnosis*; D014645:Herpesvirus 3, Human / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D008875:Middle Aged; D012680:Sensitivity and Specificity; D012698:Serologic Tests
chemical_list: D000911:Antibodies, Monoclonal