Sequential 1H and 13C resonance assignments for an octa- and decasaccharide of the N-acetyllactosamine type by multiple-step relayed correlation and heteronuclear correlation nuclear magnetic resonance.
400 MHz 1H-NMR and 100 MHz 13C-NMR spectra of a neutral octasaccharide and of a disialyldecasaccharide of the N-acetyllactosamine type were studied. The resonance assignments were made by combining multiple-relayed coherence-transfer chemical-shift-correlated spectroscopy (multiple-RELAY-COSY) and 1H/13C-shift correlated 2D experiments. The complete analysis of the 1H and 13C spectra was performed.
Wieruszeski
J M
JM
Laboratoire de Chimie Biologique, Université des Sciences et Techniques de Lille Flandres-Artois, Villeneuve d'Ascq, France.
Michalski
J C
JC
Montreuil
J
J
Strecker
G
G
D000606:Amino Sugars / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002247:Carbon Isotopes; D006801:Humans; D006859:Hydrogen; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D009083:Mucopolysaccharidoses / Q000652:urine; D009085:Mucopolysaccharidosis IV / Q000652:urine; D009844:Oligosaccharides / Q000737:chemistry* / Q000652:urine; D012794:Sialic Acids / Q000032:analysis*
D000606:Amino Sugars; D002247:Carbon Isotopes; D009844:Oligosaccharides; D012794:Sialic Acids; C000458:N-acetyllactosamine; D006859:Hydrogen