[Biotinylated Y chromosome specific probe for human sexing].
Human chromosome DNA from WBC or fetus chorion samples were digested with Hae III and hybridized with biotinylated Y chromosome specific probe by Southern blotting, and hybridization signals were developed by the ABC (Avidin-biotin-alkaline phosphatase complex) system. The hybridization signal for 0.1 microgram of male DNA could be detected clearly, while the signal for even 5 micrograms of female DNA could not. Parallel tests showed that the sexing results using 32P-labeled and biotinylated Y probe were identical. This suggests that the biotinylated Y probe can be applied to the determination of X-linked genetic diseases and sex abnormality, forensic analysis, sex determination of sportsmen and women, heterosexual transplantation of bone marrow, etc. It could become a convenient means for genetic diagnosis.
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D000345:Affinity Labels; D001386:Azides; D001710:Biotin / Q000031:analogs & derivatives; D015342:DNA Probes; D006801:Humans; D012732:Sex Determination Analysis; D014998:Y Chromosome
D000345:Affinity Labels; D001386:Azides; D015342:DNA Probes; D001710:Biotin; C045549:photobiotin