title: [Molecular biology techniques for detection of delta hepatitis virus. I: Obtaining the specific diagnostic probe].
abstract: A specific probe to detect DHV genomes was developed using as a source the p alpha-1 recombinant plasmid. The probe was labelled by using the non-isotopic method which allows its longer storage. The sensitivity of this DHV probe varies between 1 to 5 pcg.
authors.affiliation: Seccion de Biología Molecular, Centro Nacional de Referencia en Inmunología Clínica.
authors.forename: F I
authors.initials: FI
authors.lastname: Toro
authors.forename: J L
authors.initials: JL
authors.lastname: Ramírez
authors.forename: N E
authors.initials: NE
authors.lastname: Bianco
authors.forename: I V
authors.initials: IV
authors.lastname: Machado
mesh_terms: D015342:DNA Probes; D004587:Electrophoresis, Agar Gel; D016679:Genome, Viral; D003699:Hepatitis D / Q000175:diagnosis* / Q000235:genetics; D003698:Hepatitis Delta Virus / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization; D010957:Plasmids / Q000235:genetics
chemical_list: D015342:DNA Probes
vernacular_title: Técnicas de biología molecular para la detección del virus de hepatitis delta I: obtención de la sonda diagnóstica específica.