Specific binding of human C-reactive protein to human monocytes in vitro.
The precise biologic function of C-reactive protein (CRP), a major acute phase protein in man, is unknown. The abilities of CRP to bind biologic substrates and to activate the C pathway, and its localization at sites of inflammation argue for an opsonic role for this protein. Such a role has been supported by recent reports of specific binding of CRP to neutrophils. Using highly purified radioiodinated human CRP, we have observed specific binding of this protein to human monocytes in vitro. The binding was reversible and rapid, with a t1/2 for the dissociation reaction of approximately 3 min. Binding was saturable at a CRP concentration of approximately 0.2 microM, with an estimated K from Scatchard analysis of 1.1 x 10(-7) M. Specific binding was calcium-dependent, with optimal binding occurring at calcium concentrations of more than 1.0 mM. No specific binding could be demonstrated to a non-adherent population of mononuclear cells (more than 80% lymphocytes). In other experiments, a 100-fold excess of human IgG failed to inhibit binding, although rabbit CRP produced competitive inhibition of binding which was quantitatively similar to human CRP. The binding was maximal at pH 7.4 and was sensitive to prior trypsin treatment of cells. These studies provide direct evidence for specific binding of soluble human CRP to human monocytes in vitro and thus provide further support for an important functional interaction of this acute phase protein with phagocytic cells in man.
Ballou
S P
SP
Department of Medicine, Case Western Reserve University, Cleveland Metropolitan General Hospital, OH 44109.
Buniel
J
J
Macintyre
S S
SS
D000943:Antigens, Differentiation / Q000032:analysis; D001665:Binding Sites; D002097:C-Reactive Protein / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007700:Kinetics; D009000:Monocytes / Q000378:metabolism*; D009504:Neutrophils / Q000378:metabolism; D011961:Receptors, Fc / Q000032:analysis; D017452:Receptors, IgG; D014357:Trypsin / Q000494:pharmacology
D000943:Antigens, Differentiation; D011961:Receptors, Fc; D017452:Receptors, IgG; D002097:C-Reactive Protein; D014357:Trypsin; D002118:Calcium