Serological study of yeast killer toxins by monoclonal antibodies.
Yeast killer toxins coded by determined and undetermined killer plasmids or presumptive nuclear gene(s) in various genera (Saccharomyces, Kluyveromyces, Pichia and Candida) have been serologically investigated by a monoclonal antibody (KT4), produced against the yeast killer toxin of Pichia (Hansenula) anomala UCSC 25F. Double immunodiffusion with the killer toxins as antigens and indirect immunofluorescence on whole cells of the corresponding killer yeast have been used. In both the serological procedures, monoclonal antibody KT4 proved to be reacting only with the killer toxins and the whole cells of yeasts belonging to the genus Pichia.
Polonelli
L
L
Istituto di Microbiologia, Universitá di Parma, Italy.
Manzara
S
S
Conti
S
S
Dettori
G
G
Morace
G
G
Chezzi
C
C
D000911:Antibodies, Monoclonal / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D005656:Fungal Proteins / Q000276:immunology*; D005779:Immunodiffusion; D055646:Killer Factors, Yeast; D007716:Kluyveromyces / Q000276:immunology; D009183:Mycotoxins / Q000276:immunology*; D010843:Pichia / Q000276:immunology*; D012441:Saccharomyces cerevisiae / Q000276:immunology; D029701:Saccharomyces cerevisiae Proteins; D004718:Saccharomycetales / Q000276:immunology*
D000911:Antibodies, Monoclonal; D005656:Fungal Proteins; C527157:KHR1 protein, S cerevisiae; D055646:Killer Factors, Yeast; D009183:Mycotoxins; D029701:Saccharomyces cerevisiae Proteins