title: T-cell-mediated clearance of mouse hepatitis virus strain JHM from the central nervous system.
abstract: Clearance of the neurotropic JHM strain of mouse hepatitis virus from the central nervous system was examined by the transfer of spleen cells from immunized donors. A T cell with the surface phenotype of Thy1.2+ CD4+ CD8- asialo-GM1+ Mac-1- was found to be necessary for viral clearance. The surface phenotype and adherence to nylon wool suggest that these cells are activated helper-inducer T cells. Adoptive transfer to congenic histocompatibility strains demonstrated the necessity for compatibility at the D locus of the major histocompatibility complex. The expression of the CD4 surface marker and the requirement for major histocompatibility complex class I were further studied by the transfer of cells to recipients treated with anti-CD4 or anti-CD8 monoclonal antibodies. Treatment of recipients with either the anti-CD8 or the anti-CD4 antibodies inhibited virus clearance from the central nervous system. This suggests that the CD4+ cell acts as a helper and that virus is cleared from the central nervous system. This suggests that the CD4+ cell acts as a helper and that virus is cleared from the central nervous system by CD8+ cells that recognize viral antigen in the context of the H-2Db gene product.
authors.affiliation: Department of Microbiology, University of Southern California School of Medicine, Los Angeles 90033.
authors.forename: M A
authors.initials: MA
authors.lastname: Sussman
authors.forename: R A
authors.initials: RA
authors.lastname: Shubin
authors.forename: S
authors.initials: S
authors.lastname: Kyuwa
authors.forename: S A
authors.initials: SA
authors.lastname: Stohlman
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use; D000954:Antigens, Surface / Q000032:analysis; D000956:Antigens, Viral / Q000276:immunology; D002460:Cell Line; D002490:Central Nervous System / Q000276:immunology / Q000382:microbiology*; D006524:Hepatitis, Viral, Animal / Q000276:immunology*; D006968:Hypersensitivity, Delayed; D007111:Immunity, Cellular; D007116:Immunization, Passive; D051379:Mice; D008815:Mice, Inbred Strains; D006517:Murine hepatitis virus / Q000276:immunology*; D010641:Phenotype; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000956:Antigens, Viral