Detection of fos oncogene products by monoclonal antibody FO-120 in lymphoproliferative disorders.
We investigated the expression of fos oncogene proteins in lymphoproliferative disorders, using a monoclonal antibody (FO-120) that was prepared against a synthetic oligopeptide of fos protein (amino acid sequence from 127 to 152). Although peripheral blood leukocytes were rarely positive for FO-120, they were transiently stained after lectin (PHA) stimulation. After culture with IL-2 for 1 or 2 weeks, less than 40% of the lymphocytes weakly reacted with FO-120, whereas strongly positive cells were detected in more than 70% of cells in half the T-cell lines established from preleukemic state of adult T-cell leukemia (pre-ATL) and all of ATL derived T-cell lines. All in vivo specimens of non-Hodgkin's malignant lymphomas, except for one case of T-cell lymphoma were also strongly positive. In addition, the extent of the antibody reactivity correlated with the histopathological grade of malignancy in B-cell lymphoma. The reactivity to most AILD-IBL lesions overlapped with that to T-lymphomas, and could be distinguished from that to reactive lesions. FO-120 appears to be a useful tool for detecting early neoplastic changes in lymphoproliferative disorders.
Kanaitsuka
T
T
Department of Pathology, Kyoto Prefectural University of Medicine, Japan.
Namba
Y
Y
Zu
Y L
YL
Ishii
K
K
Ashihara
T
T
Hanaoka
M
M
Suchi
T
T
D000911:Antibodies, Monoclonal / Q000276:immunology; D015490:HTLV-I Infections / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D008199:Lymphadenitis / Q000378:metabolism / Q000473:pathology; D008228:Lymphoma, Non-Hodgkin / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008232:Lymphoproliferative Disorders / Q000378:metabolism* / Q000473:pathology; D011518:Proto-Oncogene Proteins / Q000276:immunology / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos