title: Immunophenotypic analysis of peripheral blood leukocytes at different stages of HIV infection. An analysis of asymptomatic, ARC, and AIDS populations.
abstract: Blood leukocytes from 51 patients with acquired immune deficiency syndrome (AIDS) or AIDS-related syndrome (ARC) were immunophenotyped with the use of monoclonal antibodies and flow cytometry. The patients were placed into four clinically defined groups: HIV-positive asymptomatic (HIV+/A, 8); persistent generalized adenopathy (14); Kaposi's sarcoma (12); and opportunistic infections (17). Immunophenotypes were compared between groups. Statistically significant differences were seen in absolute lymphocyte counts, total T-cells, helper/inducer T-cells, the helper inducer subset of CD4+ lymphocytes, the suppressor inducer subset of CD4+ lymphocytes, activated helper T-cells, and natural killer cells. CD8+ cells and subsets were not statistically different between groups, possibly obscured by large ranges, but median values suggested differences. Results indicate a pattern of increasing or decreasing numbers of certain subpopulations as HIV infection progresses.
authors.affiliation: Department of Medicine, University of Southern California School of Medicine, Los Angeles.
authors.forename: S C
authors.initials: SC
authors.lastname: Formenti
authors.forename: R R
authors.initials: RR
authors.lastname: Turner
authors.forename: R M
authors.initials: RM
authors.lastname: de Martini
authors.forename: D C
authors.initials: DC
authors.lastname: Boone
authors.forename: P C
authors.initials: PC
authors.lastname: Bishop
authors.forename: A M
authors.initials: AM
authors.lastname: Levine
authors.forename: J W
authors.initials: JW
authors.lastname: Parker
mesh_terms: D000386:AIDS-Related Complex / Q000097:blood* / Q000473:pathology; D000163:Acquired Immunodeficiency Syndrome / Q000097:blood* / Q000235:genetics / Q000473:pathology; D000328:Adult; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D006679:HIV Seropositivity / Q000097:blood* / Q000473:pathology; D006801:Humans; D007158:Immunologic Techniques; D007958:Leukocyte Count; D007962:Leukocytes / Q000145:classification* / Q000473:pathology; D008213:Lymphocyte Activation; D008297:Male; D010641:Phenotype; D013601:T-Lymphocytes / Q000145:classification / Q000473:pathology; D006377:T-Lymphocytes, Helper-Inducer / Q000145:classification
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte