[Identification of specific vasopressin binding sites in cell membranes of the adenohypophysis, liver, kidney and adrenal glands of the rat].
3H vasopressin specifically binds to the binding sites in liver, kidney and adenohypophysis with Bmax = 11.8 +/- 5.6, 1.7 +/- 0.5 and 4.9 +/- 1.2 pmol/g tissue and Kd = 1.5 +/- 0.5, 0.66 +/- 0.21 and 0.84 +/- 0.21 nM, correspondingly. Specific binding increases in the presence of Mg2+ and Ni2+ and decreases at high temperature (37 degrees). The presence of high affinity binding sites for 3H vasopressin was shown in the rat adrenals, binding was saturable and reversible. Concentration of vasopressin binding sites in adrenals is 6-8 fold less than in adenohypophysis. It is supposed that vasopressin receptors in adrenals may participate in the regulation of corticosteroid secretion.
Korneev
A Ia
AIa
Bragin
E O
EO
D000305:Adrenal Cortex Hormones / Q000378:metabolism; D000311:Adrenal Glands / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D051381:Rats; D011945:Receptors, Angiotensin / Q000502:physiology; D017483:Receptors, Vasopressin; D014667:Vasopressins / Q000378:metabolism*
D000305:Adrenal Cortex Hormones; D011945:Receptors, Angiotensin; D017483:Receptors, Vasopressin; D014667:Vasopressins
Vyiavlenie mest spetsificheskogo sviazyvaniia vazopressina na membranakh kletok adenogipofiza, pecheni, pochek i nadpochechnikov krysy.