The linear tetrasaccharide, Gal beta 1-4GlcNAc beta 1-6Gal beta 1-4GlcNAc, isolated from radiolabeled teratocarcinoma poly-N-acetyllactosaminoglycan resists the action of E. freundii endo-beta-galactosidase.
A novel linear tetrasaccharide, Gal beta 1-4GlcNAc beta 1-6Gal beta 1-4GlcNAc, was isolated from partial acid hydrolysates of metabolically labeled poly-N-acetyllactosaminoglycans of murine teratocarcinoma cells. It was characterized by exo-glycosidase sequencing and by mild acid hydrolysis followed by identification of all partial cleavage products. The tetrasaccharide, and likewise labelled GlcNAc beta 1-6Gal beta 1-4GlcNAc, resisted the action of endo-beta-galactosidase (EC 3.2.1.103) from E. freundii at a concentration of 125 mU/ml, while the isomeric, radioactive teratocarcinoma saccharides Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4GlcNAc and GlcNAc beta 1-3Gal beta 1-4GlcNAc were cleaved in the expected manner.
Renkonen
O
O
Department of Biochemistry, University of Helsinki, Finland.
Penttilä
L
L
Makkonen
A
A
Niemelä
R
R
Leppänen
A
A
Helin
J
J
Vainio
A
A
D000818:Animals; D002240:Carbohydrate Sequence; D002250:Carbon Radioisotopes; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002854:Chromatography, Paper; D004925:Escherichia / Q000201:enzymology*; D006026:Glycoside Hydrolases; D051379:Mice; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D011865:Radioisotope Dilution Technique; D013724:Teratoma / Q000737:chemistry*; D001616:beta-Galactosidase / Q000378:metabolism*
D002250:Carbon Radioisotopes; D009844:Oligosaccharides; D011134:Polysaccharides; D006026:Glycoside Hydrolases; C026093:keratan-sulfate endo-1,4-beta-galactosidase; D001616:beta-Galactosidase