[The role of thrombocyte prostanoids and products secreted by dense granules in the platelet attachment, spreading and aggregation on collagen substrates].
The role of platelet prostanoids and substances released from dense bodies (ADP and serotonin) in the initial attachment, spreading and aggregation of platelets on surfaces coated with I, III, IV and V genetic types of collagen was investigated. A positive linear correlation was found to exist between thrombi-like aggregate formation on collagen substrates and platelet prostanoid synthesis. No correlation was established between platelet aggregate formation and 14C-serotonin release. The cyclooxygenase inhibitor indomethacin and the antagonists of PG endoperoxides and TXA2 (13-APA and BM 13.177) completely block thrombi-like aggregate formation. Neither 13-APA nor BM 13.177 affect platelet spreading, while indomethacin inhibits this process by 25%. The ADP-scavenger CP/CPK inhibits platelet aggregation and spreading by 25-30%. The inhibitors of cyclooxygenase and CP/CPK do not influence the initial attachment of platelets. The data obtained suggest that thrombi-like aggregate formation on collagen substrates is mediated by the synthesis of PG endoperoxides and TXA2; however, in platelet spreading this synthesis plays a limited role. Spreading and aggregation of platelets on collagen substrates is only partly mediated by ADP and serotonin. Initial attachment of platelets does not depend on ADP and serotonin release and PG endoperoxide/TXA2 synthesis.
Leĭtin
V L
VL
Missel'vits
F
F
Liubimova
E V
EV
Domogatskiĭ
S P
SP
D000244:Adenosine Diphosphate / Q000378:metabolism; D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism*; D003094:Collagen; D016861:Cyclooxygenase Inhibitors; D003594:Cytoplasmic Granules / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D008315:Malondialdehyde / Q000378:metabolism; D010973:Platelet Adhesiveness / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors; D011449:Prostaglandin Endoperoxides / Q000502:physiology*; D012701:Serotonin / Q000378:metabolism; D013379:Substrate Specificity
D016861:Cyclooxygenase Inhibitors; D010975:Platelet Aggregation Inhibitors; D011449:Prostaglandin Endoperoxides; D012701:Serotonin; D008315:Malondialdehyde; D000244:Adenosine Diphosphate; D003094:Collagen; D007213:Indomethacin
Rol' trombotsitarnykh prostanoidov i produktov, sekretiruemykh iz plotnykh granul, v prikreplenii, rasplastyvanii i agregatsii trombotsitov na kollagenovykh substratakh.