Structure and membrane topology of the high-affinity receptor for human IFN-gamma: requirements for binding IFN-gamma. One single 90-kilodalton IFN-gamma receptor can lead to multiple cross-linked products and isolated proteins.
We analyzed the high affinity receptor for IFN-gamma of Raji cells and human placenta by combining Scatchard analysis, cross-linking experiments, and receptor purification. Only one high affinity binding site was found, Kd 2.1 X 10(-10). The receptor is a 90-kDa glycoprotein. However, multiple cross-linked products of 110 kDa to about 250 kDa could be generated and proteins of 90, 70, and 50 kDa could be obtained upon purification. These proteins all contained the same 90-kDa receptor, or part of it. We suggest that extensive cross-linking and/or proteolysis may explain many of the conflicting results published thus far. The extracellular domain of the 90-kDa receptor protein was highly resistant to digestion with trypsin or proteinase K. Trypsin digestion neither affected the number of binding sites per cell, nor the Kd for IFN-gamma. A cluster of sites for different proteases was found in the intracellular domain. The 50-kDa fragment created by trypsin digestion had the same characteristics as the isolated 50-kDa receptor fragment. It contained the IFN-gamma binding site and the receptor's extracellular and amino-terminal domain. N-linked glycosylation contributed about 15 kDa to its molecular mass, of which 4 kDa were attributable to sialic acid residues. O-Linked glycosylation was not detected. The number of binding sites per cell and the Kd for IFN-gamma were not affected by the presence or absence of N-linked glycosylation. The receptor contained at least one critical disulfide bridge and the reduced receptor could be reactivated in vitro.
Fountoulakis
M
M
Central Research Units, F. Hoffmann-La Roche & Co AG, Basel, Switzerland.
Kania
M
M
Ozmen
L
L
Loetscher
H R
HR
Garotta
G
G
van Loon
A P
AP
D000818:Animals; D001216:Asparagine / Q000378:metabolism; D002236:Carbohydrate Conformation; D003432:Cross-Linking Reagents / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D010450:Endopeptidases; D006031:Glycosylation; D006801:Humans; D006868:Hydrolysis; D007371:Interferon-gamma / Q000378:metabolism*; D007457:Iodine Radioisotopes; D008562:Membrane Glycoproteins / Q000302:isolation & purification* / Q000378:metabolism; D051379:Mice; D008970:Molecular Weight; D019158:N-Acetylneuraminic Acid; D011971:Receptors, Immunologic / Q000378:metabolism; D017471:Receptors, Interferon; D012794:Sialic Acids / Q000378:metabolism; D013329:Structure-Activity Relationship; D014357:Trypsin
D003432:Cross-Linking Reagents; D007457:Iodine Radioisotopes; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic; D017471:Receptors, Interferon; D012794:Sialic Acids; D001216:Asparagine; D007371:Interferon-gamma; D010450:Endopeptidases; D014357:Trypsin; D019158:N-Acetylneuraminic Acid