Internal binding of eucaryotic ribosomes on poliovirus RNA: translation in HeLa cell extracts.
Translation initiation on poliovirus mRNA in poliovirus-infected cells has been shown to occur by internal binding of ribosomes to the 5' noncoding region (J. Pelletier and N. Sonenberg, Nature [London] 334:320-325, 1988). Here we show that internal ribosome binding can occur in HeLa cell extracts in vitro. Internal binding to the 5' noncoding region of poliovirus mRNA in a bicistronic context was independent of the upstream open reading frame and did not require poliovirus proteins.
Pelletier
J
J
Department of Biochemistry, McGill University, Montreal, Quebec, Canada.
Sonenberg
N
N
D006367:HeLa Cells; D006801:Humans; D010957:Plasmids; D017955:Poliovirus / Q000235:genetics*; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*; D014158:Transcription, Genetic
D012367:RNA, Viral