title: Mechanisms of cellular invasiveness: a comparison of amnion invasion in vitro and metastatic behavior in vivo.
abstract: We employed a sensitive in vitro amnion invasion assay to examine the relationship of the invasive ability of numerous mouse and human tumor cell lines and their variants to their ability to spontaneously or artificially metastasize; we also studied possible enzymatic activities involved in the in vitro invasion process. In vitro invasive ability of tumor cells was strongly correlated with spontaneous metastatic ability from the subcutaneous site, regardless of the ability of tumor cells to form artificial metastases when introduced intravenously. However, normal nontumorigenic human trophoblast cells were also highly invasive. Various collagenase inhibitors totally abrogated amnion penetration by all invasive cells; various inhibitors of plasmin, plasminogen, and plasminogen activators prevented invasion in most, but not all, cases. Thus, amnion penetration provides a rigorous test for tumor cell invasiveness required for spontaneous metastasis in vivo, and invasiveness is strongly dependent on metalloproteinase activity, which usually follows plasmin activation.
authors.affiliation: Department of Anatomy, University of Western Ontario, London, Canada.
authors.forename: S
authors.initials: S
authors.lastname: Yagel
authors.forename: R
authors.initials: R
authors.lastname: Khokha
authors.forename: D T
authors.initials: DT
authors.lastname: Denhardt
authors.forename: R S
authors.initials: RS
authors.lastname: Kerbel
authors.forename: R S
authors.initials: RS
authors.lastname: Parhar
authors.forename: P K
authors.initials: PK
authors.lastname: Lala
mesh_terms: D000650:Amnion / Q000473:pathology; D000818:Animals; D001485:Basement Membrane / Q000473:pathology; D005966:Glucuronidase; D006026:Glycoside Hydrolases / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008666:Metalloendopeptidases / Q000378:metabolism; D051379:Mice; D003012:Microbial Collagenase / Q000378:metabolism; D009361:Neoplasm Invasiveness / Q000201:enzymology / Q000473:pathology*; D009362:Neoplasm Metastasis / Q000201:enzymology / Q000473:pathology*; D011480:Protease Inhibitors / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D000099175:Heparanase
chemical_list: D005966:Glucuronidase; D006026:Glycoside Hydrolases; D008666:Metalloendopeptidases; D003012:Microbial Collagenase; D011480:Protease Inhibitors; D000099175:Heparanase