title: Phosphofructokinase from porcine heart, liver and erythrocytes.
abstract: 1. Phosphofructokinase from porcine heart, liver and erythrocytes were purified by affinity chromatographies on Cibacron Blue Sepharose and N6-ATP agarose. 2. Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate revealed that the heart and liver enzymes consist of only one kind of subunit, namely, M and L type subunits, respectively, whereas the erythrocyte enzyme comprises of three kinds of subunits, M, L and C types. 3. Some kinetic and regulatory properties of the enzymes were also measured.
authors.affiliation: Institute of High Polymer Research, Faculty of Textile Science and Technology, Shishu University, Nagano, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Koyama
authors.forename: T
authors.initials: T
authors.lastname: Ema
authors.forename: N
authors.initials: N
authors.lastname: Hashizume
authors.forename: A
authors.initials: A
authors.lastname: Hachimori
authors.forename: E
authors.initials: E
authors.lastname: Iizuka
mesh_terms: D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000201:enzymology*; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology*; D010732:Phosphofructokinase-1 / Q000302:isolation & purification / Q000378:metabolism*; D013552:Swine
chemical_list: D007527:Isoenzymes; D010732:Phosphofructokinase-1