[Effect of active oxygen on cytoplasmic Ca2+ sequestration mechanism].
Effects of the active oxygen on the extrusion mechanism of once-increased cytoplasmic Ca2+, which causes various physiological phenomena, were investigated using different kinds of culture cells. First we found that, in response to stimulation with vitamin K (VK), various culture cells showed a decrease in cytoplasmic Ca2+ concentration. On the presumption that this phenomenon might be related to the oxidizing action of VK, we performed the same experiments using oxidizing agents such as H2O2 or KO2. They also showed a decrease in cytoplasmic Ca2+ concentration. Furthermore, they suppressed the increase of cytoplasmic Ca2+ by vasopressin. It would be inferred from these results that the active oxygen may act upon some site of the cellular signal transduction system of cell membrane to lower the cytoplasmic Ca2+ level.
Tomono
M
M
Department of Biochemistry, Toho University School of Medicine, Tokyo, Japan.
Urayama
T
T
Ueyama
M
M
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism*; D006168:Guinea Pigs; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D051379:Mice; D016877:Oxidants / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D014667:Vasopressins / Q000037:antagonists & inhibitors; D014812:Vitamin K / Q000494:pharmacology*
D016877:Oxidants; D014667:Vasopressins; D014812:Vitamin K; D006861:Hydrogen Peroxide; D002118:Calcium