Vesicular transport between the endoplasmic reticulum and the Golgi stack requires the NEM-sensitive fusion protein.
An N-ethylmaleimide-sensitive fusion protein (NSF) has been purified on the basis of its ability to catalyse vesicular transport within the Golgi stack. We report here that this same protein is required for transport from the endoplasmic reticulum to the Golgi stack in semi-intact cells. This transport process is inhibited by a monoclonal antibody against NSF. Furthermore, pretreatment of semi-intact cells with N-ethylmaleimide, a sulphydryl alkylating reagent, inhibits transport. Addition of highly purified NSF largely restores transport from endoplasmic reticulum to Golgi. These results suggest that NSF is a general component of the transport machinery required for membrane fusion at multiple stages of the secretory pathway.
Beckers
C J
CJ
Department of Molecular Biology, Scripps Clinic and Research Foundation, La Jolla, California 92037.
Block
M R
MR
Glick
B S
BS
Rothman
J E
JE
Balch
W E
WE
D000818:Animals; D001692:Biological Transport; D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D002472:Cell Transformation, Viral; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D005033:Ethylmaleimide / Q000494:pharmacology; D006056:Golgi Apparatus / Q000378:metabolism*; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000378:metabolism; D052066:N-Ethylmaleimide-Sensitive Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics; D033921:Vesicular Transport Proteins; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics; D014759:Viral Envelope Proteins / Q000378:metabolism
D002352:Carrier Proteins; C028391:G protein, vesicular stomatitis virus; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D033921:Vesicular Transport Proteins; D014759:Viral Envelope Proteins; D052066:N-Ethylmaleimide-Sensitive Proteins; D005033:Ethylmaleimide