[Ultrastructural detection of ribosomal RNA by in situ hybridization using a biotinylated probe on ultrathin sections of cultured animal cells].
A genomic probe containing ribosomal sequences and labelled with biotin was used to hybridize rRNA molecules in ultrathin sections of animal cells embedded in Lowicryl K4M. After detection with streptavidin conjugated with 10 nm gold particles, ribosomal target sequences were localized preferentially in the dense fibrillar component of the nucleolus and in the polyribosome structures of the cytoplasm. The method presently described offers the possibility to detect rapidly and precisely ribosomal gene expression at the ultrastructural level, particularly under different physiological and pathological conditions.
Escaig-Haye
F
F
Hôpital Saint-Vincent-de-Paul, I.N.S.E.R.M., U. n 43, Paris.
Grigoriev
V
V
Fournier
J G
JG
D000818:Animals; D001710:Biotin; D002466:Cell Nucleolus / Q000032:analysis / Q000648:ultrastructure; D003593:Cytoplasm / Q000032:analysis / Q000648:ultrastructure; D008722:Methods; D009693:Nucleic Acid Hybridization; D015347:RNA Probes; D012335:RNA, Ribosomal / Q000032:analysis / Q000648:ultrastructure*; D014709:Vero Cells
D015347:RNA Probes; D012335:RNA, Ribosomal; D001710:Biotin
Détection ultrastructurale d'ARN ribosomal par hybridation in situ à l'aide d'une sonde biotinylée sur coupes ultrafines de cellule animale en culture.