Fructose 2,6-bisphosphate and insulin stimulation of glycolysis in 3T3-L1 adipocytes.
1. Insulin is able to stimulate lactate production and to enhance fructose 2,6-bisphosphate (Fru-2,6-P2) content in 3T3-L1 adipocytes. 2. Phorbol 12-myristate 13-acetate is more efficacious than insulin in rising Fru-2,6-P2 content and less effective in the stimulation of glycolysis. 3. 3T3-L1 adipocyte 6-phosphofructo-l-kinase appears to be very sensitive to exogenous Fru-2,6-P2. 4. Insulin treatment does not affect the maximum activity of 6-phosphofructo-1-kinase whereas it markedly increases the affinity of pyruvate kinase for phosphoenolpyruvate. 5. The role of Fru-2,6-P2 in the insulin induced enhancement of glycolytic flux is discussed.
Vasta
V
V
Dipartimento di Scienze Biochimiche, Università di Firenze, Italia.
Bruni
P
P
Vannini
F
F
Farnararo
M
M
D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects*; D000818:Animals; D002460:Cell Line; D005347:Fibroblasts / Q000187:drug effects; D005635:Fructosediphosphates / Q000378:metabolism / Q000494:pharmacology*; D006019:Glycolysis / Q000187:drug effects*; D006598:Hexosediphosphates / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology*; D010732:Phosphofructokinase-1 / Q000378:metabolism; D011770:Pyruvate Kinase / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D005635:Fructosediphosphates; D006598:Hexosediphosphates; D007328:Insulin; D010732:Phosphofructokinase-1; D011770:Pyruvate Kinase; C029063:fructose-1,6-diphosphate; D013755:Tetradecanoylphorbol Acetate