Synchronized human diploid fibroblasts: progression capabilities of a subpopulation that fails to keep pace with the predominant, rapidly dividing cohort of cells.
Highly synchronized cultures of HSF-55 human diploid fibroblasts contain subpopulations of cells with intact plasma membranes that do not participate in the parasynchronous division wave. To determine the fate of these laggard cells, cultures were incubated with BrdU for variable periods to label newly replicated DNA in both the readily synchronizable and nonsynchronizable subpopulations. The kinetics of labeling with BrdU were determined with a two-laser flow cytometric technique that did not employ antibody to BrdU, but instead monitored emission of fluorescence from DNA-specific stains that differed in the degree of BrdU-induced quenching of their fluorescence signals. Approximately 90% of the cells rapidly incorporated BrdU and later divided within a 3 hr period. The remaining 10% of the cells, however, were found to reside within a minority subpopulation that maintained the capacity to traverse the cell cycle, but at a greatly reduced rate relative to the progression capacity of the majority of cells. Cells were viably sorted from these cohorts within the synchronized culture, and their kinetic behavior was determined through direct measurement of their growth rates and plating efficiencies. As predicted by the BrdU labeling studies, the sorted cells from the minority, slowly traversing subpopulation divided at a rate that was 30 to 50% lower than that obtained with cells sorted from the readily synchronizable subpopulation. From consideration of the kinetics of entry into S-phase of the majority and minority subpopulations, protocols are described that should allow preparation of relatively pure populations of both early- and late-replicating species of human DNA.
Tobey
R A
RA
Genetics Group, Los Alamos National Laboratory, New Mexico 87545.
Oishi
N
N
Crissman
H A
HA
D016590:Aphidicolin; D001562:Benzimidazoles; D001973:Bromodeoxyuridine; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division; D002469:Cell Separation; D002478:Cells, Cultured; D004261:DNA Replication; D004224:Diterpenes / Q000494:pharmacology; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D006801:Humans; D008926:Plicamycin
D001562:Benzimidazoles; D004224:Diterpenes; D005456:Fluorescent Dyes; D016590:Aphidicolin; D001973:Bromodeoxyuridine; D008926:Plicamycin; C017807:bisbenzimide ethoxide trihydrochloride