title: Identification of interleukin 1 alpha produced by the 3B6 human EBV-B cell line.
abstract: We have previously described a novel protein, derived from the 3B6 EBV B-cell line which apparently displayed IL-1 bioactivity. We report here that the protein previously characterized is not a new species of IL-1. Indeed, we have now been able to clone and express a cDNA which identifies this protein as human thioredoxin, an intracellular dithiol oxydo-reductase enzyme. Thioredoxin however, is not a lymphokine and in particular does not possess IL-1 activity. Our earlier data can be explained in the light of recent findings, reported herein, indicating that IL-1 alpha is produced by the 3B6 line as assessed by antibody inhibition studies and IL-1 alpha mRNA expression. Thus our original protein preparation actually contained traces of IL-1 alpha, which was responsible for the biologic activities and thioredoxin.
authors.affiliation: Laboratoire d'immunologie, UA CNRS 1156, Institut Gustave Roussy, Villejuif, France.
authors.forename: J
authors.initials: J
authors.lastname: Bertoglio
authors.forename: E
authors.initials: E
authors.lastname: Wollman
authors.forename: A
authors.initials: A
authors.lastname: Shaw
authors.forename: L
authors.initials: L
authors.lastname: Rimsky
authors.forename: D
authors.initials: D
authors.lastname: Fradelizi
mesh_terms: D000595:Amino Acid Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D004854:Herpesvirus 4, Human; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis*; D008969:Molecular Sequence Data; D013879:Thioredoxins / Q000032:analysis; D014407:Tumor Cells, Cultured / Q000032:analysis
chemical_list: D007375:Interleukin-1; D013879:Thioredoxins