The use of bromodeoxyuridine-immunohistochemistry to identify transplanted fetal brain tissue.
The immunohistochemical detection of the thymidine analog, 5-bromo-2'-deoxyuridine (BrdU) is shown to be a useful and reliable method to positively identify fetal brain transplants in standard histological preparations. This technique offers several advantages over the [3H]thymidine autoradiographic method, including being much more rapid and avoiding the use of radionuclides.
Brown
D B
DB
Laboratory of Clinical Science, National Institute of Mental Health, National Institutes of Health Animal Center, Poolesville, MD 20837.
Stanfield
B B
BB
D000818:Animals; D000831:Animals, Newborn; D001345:Autoradiography / Q000379:methods; D016380:Brain Tissue Transplantation / Q000502:physiology; D001973:Bromodeoxyuridine; D002540:Cerebral Cortex / Q000166:cytology / Q000502:physiology / Q000637:transplantation*; D016332:Fetal Tissue Transplantation / Q000502:physiology*; D007150:Immunohistochemistry / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D014316:Tritium; D001973:Bromodeoxyuridine; D013936:Thymidine