Effects of in vitro and in vivo administration of recombinant bovine interferon-gamma on bovine neutrophil responses to Brucella abortus.
The effects of in vitro and in vivo treatment of bovine polymorphonuclear leukocytes with recombinant bovine interferon-gamma on in vitro bovine polymorphonuclear leukocyte functions and the survival of Brucella abortus were determined. Activation of neutrophils in vitro with interferon-gamma resulted in enhanced production of O2- and myelopeoroxidase-H2O2-halide activity by neutrophils in the presence of B. abortus. The improved iodination responses were correlated with an enhanced ability to perform iodination in the presence of 5'-guanosine monophosphate and adenine which have previously been shown to contribute to inhibition of neutrophil myeloperoxidase-H2O2-halide activity by B. abortus. The ability of opsonized B. abortus to survive in the presence of neutrophils activated in vitro or in vivo was partially decreased by approximately 10% of control when compared to survival rates within control phagocytes. These results suggest that activation of neutrophils with recombinant interferon-gamma partially enhances their oxidative metabolic responses, resulting in a slightly enhanced ability to kill virulent B. abortus.
Canning
P C
PC
Brucellosis Research Laboratory, U.S.D.A., Ames, IA 50010.
Roth
J A
JA
D000818:Animals; D002003:Brucella abortus / Q000276:immunology*; D002417:Cattle; D002464:Cell Migration Inhibition; D002470:Cell Survival; D005260:Female; D007371:Interferon-gamma / Q000494:pharmacology*; D008297:Male; D009504:Neutrophils / Q000276:immunology*; D010084:Oxidation-Reduction; D011897:Random Allocation; D011994:Recombinant Proteins; D013481:Superoxides / Q000378:metabolism
D011994:Recombinant Proteins; D013481:Superoxides; D007371:Interferon-gamma