Evidence for vasoactive intestinal peptide receptors in apical membranes from tracheal epithelium.
[125I]VIP (vasoactive intestinal peptide) bound to apical membranes isolated from the bovine tracheal epithelium with a half maximal inhibition by unlabeled VIP (IC50) of 0.6 x 10(-9)M and binding was reversible. Glucagon did not affect [125I]VIP binding to the membranes. [125I]VIP was covalently cross-linked to tracheal membrane proteins using disuccinimidyl suberate. SDS-polyacrylamide gel electrophoresis of labeled tracheal membranes revealed one major [125I]-receptor complex of Mr = 71,000 to which binding of [125I]VIP was inhibited by 10 microM unlabeled VIP. These results are consistent with the presence of a specific, high-affinity receptor for VIP, with a Mr = 71,000, in apical membrane vesicles isolated from the bovine tracheal epithelium.
Elgavish
A
A
Department of Pharmacology, University of Alabama, Birmingham 35294.
Pillion
D J
DJ
Meezan
E
E
D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism; D005934:Glucagon / Q000494:pharmacology; D007700:Kinetics; D008970:Molecular Weight; D011964:Receptors, Gastrointestinal Hormone / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D018005:Receptors, Vasoactive Intestinal Peptide; D014132:Trachea / Q000378:metabolism*; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*
D011964:Receptors, Gastrointestinal Hormone; D018005:Receptors, Vasoactive Intestinal Peptide; D014660:Vasoactive Intestinal Peptide; D005934:Glucagon