title: Gas chromatographic determination of nicotinamide in meats and meat products as 3-cyanopyridine.
abstract: The determination of nicotinamide as 3-cyanopyridine after dehydration by heptafluorobutyric anhydride (HFB) was performed by gas-liquid chromatography (GLC) with flame ionization detection (GLC-FID) and a column of 5% OV-17 on Chromosorb W AW DMCS at 130 degrees C. Determination was possible with 3-100 micrograms of the dehydrated reaction mixture. The procedure for determining nicotinamide in various meats and meat products involves direct analysis by GLC-FID without a clean-up stage; the detection limit is 5 ppm and the recovery ranged from 93.4 to 104.6% (average 98.0%). Various possible interferents in the samples did not interfere in the production or determination of 3-cyanopyridine. The procedure is suitable for routine use. The dehydrated derivative of nicotinamide was confirmed as 3-cyanopyridine by combined gas chromatography-mass spectrometry and infrared spectrometry.
authors.affiliation: Saitama Institute of Public Health, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Tanaka
authors.forename: M
authors.initials: M
authors.lastname: Iijima
authors.forename: Y
authors.initials: Y
authors.lastname: Kikuchi
authors.forename: Y
authors.initials: Y
authors.lastname: Hoshino
authors.forename: N
authors.initials: N
authors.lastname: Nose
mesh_terms: D002849:Chromatography, Gas; D003890:Desiccation; D005410:Flame Ionization; D008460:Meat / Q000032:analysis*; D008461:Meat Products / Q000032:analysis*; D009536:Niacinamide / Q000032:analysis*; D011725:Pyridines / Q000032:analysis*
chemical_list: D011725:Pyridines; D009536:Niacinamide; C020729:3-cyanopyridine