Light microscopical visualization of phosphatase activities demonstrated with the cerium method in resin sections of the kidney with the microwave oven.
The light microscopically invisible reaction product cerium phosphate in resin sections of rat kidney, that had been incubated for the demonstration of phosphatase activities before embedding, was converted into a visible reaction product by incubation for 10 min at 80 degrees C in alkaline lead citrate in a microwave oven. This method offers the possibility to study phosphatase activities with the cerium method in semithin Epon sections. Furthermore it is a suitable method to select areas with phosphatase activity to be studied with the electron microscope.
Kalicharan
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Centre for Medical Electron Microscopy, University of Groningen, The Netherlands.
Hulstaert
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CE
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D002563:Cerium; D007668:Kidney / Q000166:cytology / Q000201:enzymology*; D007687:Kidney Tubules, Proximal / Q000166:cytology / Q000201:enzymology / Q000648:ultrastructure; D008297:Male; D008871:Microvilli / Q000166:cytology / Q000201:enzymology / Q000648:ultrastructure; D008872:Microwaves; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D002563:Cerium; D010744:Phosphoric Monoester Hydrolases; D000251:Adenosine Triphosphatases