title: Restricted clonality of visceral sarcomas in avian sarcoma virus-infected chickens.
abstract: Experiments were undertaken to analyze proviral DNA in primary (wing web) and visceral sarcomas arising in FP chickens infected with BH-RSV(RAV-2). Using the degree of heterogeneity of BH-RSV proviral integration sites as a measure of the degree of polyclonality of sarcoma tissue, we observed that a high proportion of the visceral sarcomas examined comprised dominant clones, independently of whether these sarcomas were isolated from immune-suppressed or nonsuppressed infected chickens; by contrast, a marked heterogeneity of BH-RSV proviral integration sites was noted with primary sarcoma tissue. Several visceral sarcomas containing dominant clones were characterized by the integration of a deleted form of the BH-RSV provirus. In addition, all of the primary and visceral sarcomas exhibited sequences specific for the RAV-2 provirus, and both types of sarcoma tissue were competent for infectious sarcoma virus production.
authors.affiliation: Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104.
authors.forename: M S
authors.initials: MS
authors.lastname: Halpern
authors.forename: S B
authors.initials: SB
authors.lastname: McMahon
authors.forename: F
authors.initials: F
authors.lastname: Branco
mesh_terms: D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D015139:Blotting, Southern; D002645:Chickens; D002999:Clone Cells; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis*; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D015252:Deoxyribonucleases, Type II Site-Specific; D011533:Proviruses / Q000235:genetics*; D015183:Restriction Mapping; D001357:Sarcoma, Avian / Q000209:etiology / Q000382:microbiology*
chemical_list: D015342:DNA Probes; D004279:DNA, Viral; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; C024408:endodeoxyribonuclease SacI; D015252:Deoxyribonucleases, Type II Site-Specific