13N isotope studies of glutamine assimilation pathways in Neurospora crassa.
L-[amide-13N]glutamine in Neurospora crassa is metabolized to [13N]glutamate by glutamate synthase and to [13N]ammonium by the glutamine transaminase-omega-amidase pathway. The [13N]ammonium released is assimilated by glutamate dehydrogenase and glutamine synthetase, confirming the operation of a glutamine cycle. Most of the nitrogen is retained during cycling between glutamate and glutamine.
Calderón
J
J
Departamento de Ecologia Molecular, Universidad Nacional Autónoma de México, Morelos.
Cooper
A J
AJ
Gelbard
A S
AS
Mora
J
J
D000641:Ammonia / Q000032:analysis; D005973:Glutamine / Q000378:metabolism*; D009154:Mutation; D009491:Neurospora / Q000378:metabolism*; D009492:Neurospora crassa / Q000235:genetics / Q000378:metabolism*; D009590:Nitrogen Radioisotopes; D011865:Radioisotope Dilution Technique; D013045:Species Specificity
D009590:Nitrogen Radioisotopes; D005973:Glutamine; D000641:Ammonia