title: Effect of cromakalim on smooth muscle cells of guinea-pig taenia caeci.
abstract: Cromakalim caused hyperpolarization and reduction of the electrotonic potential in a concentration-dependent manner in smooth muscle cells of guinea-pig taenia caeci. There was a relatively constant change in the electrotonic potential under calcium-free, low-sodium and low-chloride conditions in the presence of cromakalim as compared to control conditions with Krebs solutiuon. The effect of cromakalin (10(-5) M) was inhibited by glibenclamide (5 X 10(-5) M). These results indicate that cromakalim specifically promotes potassium efflux in smooth muscle cells of the guinea-pig taenia caeci via glibenclamide-sensitive potassium channels, to cause hyperpolarization, suppression of spike activity and relaxation.
authors.affiliation: Department of Pharmacology/Clinical Pharmacology, University of Groningen, The Netherlands.
authors.forename: A
authors.initials: A
authors.lastname: den Hertog
authors.forename: J
authors.initials: J
authors.lastname: Van den Akker
authors.forename: A
authors.initials: A
authors.lastname: Nelemans
mesh_terms: D000818:Animals; D001578:Benzopyrans / Q000494:pharmacology*; D002432:Cecum / Q000187:drug effects; D019806:Cromakalim; D004594:Electrophysiology; D005260:Female; D005905:Glyburide / Q000494:pharmacology; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000166:cytology / Q000187:drug effects*; D010276:Parasympatholytics / Q000494:pharmacology*; D011758:Pyrroles / Q000494:pharmacology*
chemical_list: D001578:Benzopyrans; D010276:Parasympatholytics; D011758:Pyrroles; D019806:Cromakalim; D005905:Glyburide