Chloride transport in human fibroblasts is activated by hypotonic shock.
Incubation of human skin fibroblasts in hypotonic media induced the activation of 36Cl- efflux which was roughly proportional to the decrease in the osmolality of the media. The efflux of 36Cl- was insensitive to DIDS plus furosemide and inhibited by addition of a Cl- channel blocker such as 5-nitro-2-(3-phenyl propylamino) benzoic acid (NPPB). We propose that a conductive pathway for Cl- transport, almost silent in isotonic conditions, is activated by exposing human fibroblasts to hypotonic shock, this conclusion being supported by evidence that also 36Cl- influx was enhanced by hypotonic medium.
Rugolo
M
M
Dipartimento di Biologia Evoluzionistica e Sperimentale, Universita' di Bologna, Italy.
Mastocola
T
T
Flamigni
A
A
Lenaz
G
G
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D018118:Chloride Channels; D002712:Chlorides / Q000378:metabolism*; D002713:Chlorine; D005347:Fibroblasts / Q000378:metabolism*; D005665:Furosemide / Q000494:pharmacology; D006801:Humans; D007038:Hypotonic Solutions; D007700:Kinetics; D008565:Membrane Proteins / Q000378:metabolism; D009579:Nitrobenzoates / Q000494:pharmacology; D009994:Osmolar Concentration; D009997:Osmotic Pressure; D011868:Radioisotopes
D018118:Chloride Channels; D002712:Chlorides; D007038:Hypotonic Solutions; D008565:Membrane Proteins; D009579:Nitrobenzoates; D011868:Radioisotopes; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; C058176:5-nitro-2-(3-phenylpropylamino)benzoic acid; D002713:Chlorine; D005665:Furosemide; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid