title: Regeneration of functional and activated NK and T sub-subset cells in the marrow and blood after autologous bone marrow transplantation: a prospective phenotypic study with 2/3-color FACS analysis.
abstract: The phenotypic reconstitution of lymphoid cells in the bone marrow and peripheral blood was examined prospectively in 27 patients who underwent autologous bone marrow transplantation (ABMT) for leukemia/lymphoma. Patterns of activation within NK and T cell subsets as well as T sub-subsets were studied with monoclonal antibodies in two- and three-color FACS analysis. NK-like cells (CD16+) were found to be increased in the peripheral blood and bone marrow after ABMT. The expression of two activation markers, 4F2 and HLA-DR, was sustainedly increased within this subset. High numbers of CD4+ and CD8+ T cells carrying surface HLA-DR were found early after ABMT both in blood and marrow. The T suppressor inducer cell sub-subset (CD45R+ CD4+) was severely depressed in both the blood and marrow 6-9 months post-ABMT. Using three-color FACS analysis, half of this T sub-subset was shown to express HLA-DR+. The levels of T suppressor effector-like cells (CD11+ CD8+) remained within the normal range in both peripheral blood and bone marrow during follow-up. The HLA-DR expression was elevated and equally distributed between the CD11- CD8+ and CD11+ CD8+ sub-subset cells. There was no major impact of marrow T cell purging or CMV carrier status on the phenotypic NK/T cell reconstitution. The present results provide an immune phenotypic basis for the suggested generation of anti-leukemic NK-like and T suppressor-like activity after ABMT.
authors.affiliation: Clinical Immunology Section, University Hospital, Uppsala, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Bengtsson
authors.forename: T H
authors.initials: TH
authors.lastname: Tötterman
authors.forename: B
authors.initials: B
authors.lastname: Smedmyr
authors.forename: R
authors.initials: R
authors.lastname: Festin
authors.forename: G
authors.initials: G
authors.lastname: Oberg
authors.forename: B
authors.initials: B
authors.lastname: Simonsson
mesh_terms: D000293:Adolescent; D000328:Adult; D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000032:analysis; D001853:Bone Marrow / Q000032:analysis / Q000502:physiology; D016026:Bone Marrow Transplantation; D002353:Carrier State / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D003586:Cytomegalovirus Infections / Q000276:immunology; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007694:Killer Cells, Natural / Q000145:classification* / Q000276:immunology / Q000502:physiology; D008213:Lymphocyte Activation; D008212:Lymphocyte Depletion; D008875:Middle Aged; D010641:Phenotype; D011446:Prospective Studies; D012038:Regeneration; D013601:T-Lymphocytes / Q000145:classification* / Q000276:immunology / Q000502:physiology; D050378:T-Lymphocytes, Regulatory / Q000145:classification
chemical_list: D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation