Replication of adeno-associated virus in cells irradiated with UV light at 254 nm.
Irradiation of simian virus 40 (ori mutant)-transformed Chinese hamster embryo cells (OD4 line) with UV light induced a cellular capacity which supported a full cycle of helper-independent adeno-associated virus replication. Monochromatic UV light at 254 nm was about 1,000-fold more effective than UV light at 313 nm, indicating that cellular nucleic acid is the primary chromophore in the UV-induced process leading to permissiveness for adeno-associated virus replication. The UV irradiation and the infection could be separated for up to 12 h without substantial loss of permissiveness. During this time interval, the induction process was partly sensitive to cycloheximide, suggesting a requirement for de novo protein synthesis.
Yakobson
B
B
Department of Virology, Weizmann Institute of Science, Rehovot, Israel.
Hrynko
T A
TA
Peak
M J
MJ
Winocour
E
E
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured / Q000528:radiation effects; D006224:Cricetinae; D003513:Cycloheximide / Q000494:pharmacology; D000229:Dependovirus / Q000254:growth & development*; D004307:Dose-Response Relationship, Radiation; D013997:Time Factors; D014466:Ultraviolet Rays; D014779:Virus Replication / Q000187:drug effects / Q000528:radiation effects*
D003513:Cycloheximide