The spliced BZLF1 gene of Epstein-Barr virus (EBV) transactivates an early EBV promoter and induces the virus productive cycle.
A spliced cDNA spanning the Epstein-Barr virus BZLF1 gene expresses the BZLF1 protein and is active in inducing the virus productive cycle. A deletion mutant which lacks the N-terminal half of the protein is inactive. Cotransfection experiments in EBV-negative B-lymphocyte cell lines demonstrated that the BZLF1 gene activates the promoter for the BSLF2 + BMLF1 gene in the absence of any other EBV gene product. These results confirmed that the spliced BZLF1 gene is the transactivating gene structure in BamHI-Z.
Rooney
C M
CM
Ludwig Institute for Cancer Research, St. Mary's Hospital Medical School, London, England.
Rowe
D T
DT
Ragot
T
T
Farrell
P J
PJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D004274:DNA, Recombinant; D005786:Gene Expression Regulation; D005796:Genes; D005814:Genes, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000502:physiology; D006801:Humans; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014162:Transfection; D014779:Virus Replication
D004274:DNA, Recombinant; D009856:Oncogene Proteins, Viral; D012333:RNA, Messenger