Identification and preliminary use of recombinant lambda gt11 fusion proteins in human cytomegalovirus diagnosis.
We have isolated reactive clones from a lambda gt11 expression library of human cytomegalovirus (HCMV) DNA using HCMV-positive human sera. Among the recombinant clones obtained, one carried a fragment encoding a portion of p52, the major non-structural DNA-binding protein of 52K (p52) and another carried a part of the gene coding for pl50, the major structural phosphoprotein. These two fusion proteins were examined by immunoblot analysis to test their ability to bind specific antibodies in human sera. The results showed that high titres of antibody to the DNA-binding protein are present in sera of patients undergoing acute HCMV infection, whereas high titres of antibodies to the structural phosphoprotein are widespread in the healthy HCMV-seropositive population. The use of these fusion proteins as antigens for differential screening of serum as a way of detecting recent HCMV infection is discussed.
Ripalti
A
A
Institute of Microbiology, Medical Faculty, S. Orsola General Hospital, Bologna, Italy.
Landini
M P
MP
Mocarski
E S
ES
La Placa
M
M
D000914:Antibodies, Viral / Q000032:analysis; D003001:Cloning, Molecular; D003587:Cytomegalovirus / Q000235:genetics* / Q000276:immunology; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D004279:DNA, Viral / Q000235:genetics / Q000276:immunology; D005814:Genes, Viral; D006801:Humans; D015151:Immunoblotting; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D015183:Restriction Mapping; D014760:Viral Fusion Proteins / Q000235:genetics / Q000276:immunology
D000914:Antibodies, Viral; D004279:DNA, Viral; D011994:Recombinant Proteins; D014760:Viral Fusion Proteins