[Features of expression of cloned genes under the control of tandem promotors pL and pR of phage lambda].
The regulatory block P'R from the bacteriophage lambda has been inserted between the promoter and initial part of the gene into the plasmid pCJ55 carrying the gene for the Klenow fragment under the control of pL. As it should be predicted, at the inverted orientation the sharp decrease in the Klenow fragment quantity is registered. However, at the direct orientation there is some decrease in the synthesis of the protein, as compared with the synthesis of the Klenow fragment in the strain harbouring the plasmid pCJ55. A plausible explanation of the fact may be in the transcriptional interference of the promoters pL and p'R in artificially constructed structures.
Zheleznaia
L A
LA
Troianovskaia
I N
IN
Stiaponavichute
D V
DV
Matvienko
N I
NI
D010582:Bacteriophage lambda / Q000235:genetics*; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D005814:Genes, Viral; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
Ossobennosti ékspressii klonirovannykh genov pod kontrolem tandema promotorov pL and pR faga lambda.