Insulin-induced expression of human heat-shock protein gene hsp70.
In human hepatoma cell line Hep3B/T2, the human heat-shock-inducible gene hsp70 could be induced by insulin. The dose-dependent insulin effect correlates very well with the dissociation constant of the insulin receptor, indicating that the insulin effect is mediated by the insulin receptor. The expression of hsp70 gene was neither significantly induced by growth factors of epidermal and platelet-derived growth factors, nor by tumor promoter, calcium ionophore, cAMP, and glucocorticoids. These results indicate that the induction of expression of hsp70 gene by insulin is very specific and not cell cycle-related. Furthermore, the insulin-induced expression of hsp70 gene is transient, occurring specifically from 4 to 8 h after insulin addition.
Ting
L P
LP
Graduate Institute of Microbiology and Immunology, National Yang-Ming Medical College, Taipei, Taiwan.
Tu
C L
CL
Chou
C K
CK
D006528:Carcinoma, Hepatocellular; D003513:Cycloheximide / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects*; D006360:Heat-Shock Proteins / Q000235:genetics*; D006514:Hepatitis B Surface Antigens / Q000235:genetics; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D007700:Kinetics; D008113:Liver Neoplasms; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics; D011972:Receptor, Insulin / Q000187:drug effects / Q000378:metabolism; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D006360:Heat-Shock Proteins; D006514:Hepatitis B Surface Antigens; D007328:Insulin; D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D004815:Epidermal Growth Factor; D004247:DNA; D003513:Cycloheximide; D011972:Receptor, Insulin