[Quantitative determination of the rate of superoxide radical formation in mitochondrial membranes by electron paramagnetic resonance].
The reaction of hydroxylamine (1-hydroxy-2,2,6,6-tetramethyl-4-oxopiperidine) with O2-. resulting in the stable nitroxyl radical formation recorded by ESR-technique was applied to estimate quantitatively the rate of O2-. superoxide radical generation (VO2.-) by submitochondrial particles (SMP) of liver (of mice and rats). The VO2.- dependence on concentrations of NADH, succinate and protein of SMP was established. The method allows detecting VO2.- greater than 0.05 nmol.min-1.ml-1. It has been shown that in the NADH-dependent site of the chain VO2.- is 3-4 times that in the succinate-dependent site. In the presence of rhotenone and antimycin A VO2.- increases by 30-35%, while cyanide retards VO2.- by about 30%. The data comparison with regard to VO2.- and O2 absorption rate polarographically determined has indicated that about 2% of the absorbed O2 is consumed to form O2-.
Rashba
Iu E
IuE
Vartanian
L S
LS
Baĭder
L M
LM
Krinitskaia
L A
LA
D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D003486:Cyanides / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D007425:Intracellular Membranes / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D008930:Mitochondria, Liver / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D012402:Rotenone / Q000494:pharmacology; D013386:Succinates / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*
D003486:Cyanides; D005609:Free Radicals; D013386:Succinates; D012402:Rotenone; D009243:NAD; D013481:Superoxides; D000968:Antimycin A
Kolichestvennoe opredelenie skorosti generirovaniia superoksidnykh radikalov v mitokhondrial'nykh membranakh metodom élektronnogo paramagnitnogo rezonansa.