Immuno- and enzyme-histochemical detection of phosphoprotein phosphatase in rat epidermis.
A phosphoprotein phosphatase (PPase: EC 3.1.3.2) was recently purified from rat epidermis. The enzyme dephosphorylates phosphoprotein, and its properties, such as pH optimum, inhibitor spectrum, and Fe2+ activation, differ from those of other soluble phosphatases. We investigated in 2-day-old rat skin the distribution of immunologically detectable PPase and intracellular localization of PPase activity. The reaction of rabbit monospecific anti-PPase IgG was identified in granular and cornified cells by the avidin-biotin complex method. For activity staining, basic principles of the Gomori lead-salt method and azo dye technique with the substrates p-nitrophenylphosphate (p-NPP) and alpha-naphthyl phosphate (NP), respectively, were modified according to the biochemical properties of PPase activity which is resistant to formalin, Na tartrate, and NaF. Activity was detectable in granular cells including keratohyalin granules and the lower strata of cornified cells. The activity was inhibited by 1 mM CuSO4 and enhanced by a mixture of 0.5 mM FeSO4 and 1 mM ascorbic acid. We consider that PPase may be involved in dephosphorylation of histidine-rich proteins in granular and cornified cells and may play a key role in intracellular catabolism associated with epidermal cell differentiation.
Ohno
J
J
Department of Dermatology, University of California, San Francisco 94143.
Fukuyama
K
K
Hara
A
A
Epstein
W L
WL
D000818:Animals; D000078404:Epidermal Cells; D004817:Epidermis / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D007501:Iron / Q000378:metabolism; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D007501:Iron; D010749:Phosphoprotein Phosphatases