The germline repertoire of T-cell receptor beta-chain genes in patients with multiple sclerosis.
The T-cell receptor (TCR) beta-chain gene repertoire of 40 multiple sclerosis (MS) patients was compared to that of 100 normal individuals. V-beta probes that represent 14 different V-beta subfamilies plus a C-beta probe were used to identify 53 separate beta-chain gene segments. No duplication or deletion of any of these 53 gene segments was found in the MS patients. Restriction fragment length polymorphism (RFLP) alleles detected by V-beta 8, V-beta 11 and C-beta probes defined 8 different beta-chain haplotypes. The distribution of these haplotypes in Caucasian MS patients and normal individuals was significantly different (p = 0.012). Comparison of the DR2+ subset of MS patients (n = 32) to a second group of 43 Caucasian DR2+ normal individuals revealed that the distribution of these beta-chain haplotypes was significantly different in these two populations (p = 0.015). These results suggest that an MS susceptibility gene(s) may be located in the region of the TCR/beta-chain gene complex.
Biddison
W E
WE
Neuroimmunology Branch, National Institute of Neurological, Communicative Disorders, and Stroke, Bethesda, MD 20892.
Beall
S S
SS
Concannon
P
P
Charmley
P
P
Gatti
R A
RA
Hood
L E
LE
McFarland
H F
HF
McFarlin
D E
DE
D000483:Alleles; D005787:Gene Frequency; D006239:Haplotypes; D006801:Humans; D009103:Multiple Sclerosis / Q000235:genetics / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D016693:Receptors, Antigen, T-Cell, alpha-beta
D011948:Receptors, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta