A technique for rapid purification of low yield products: biotinylation of chemically synthesized proteins on-resin.
We report here straightforward methodology for the purification of chemically synthesized proteins which are produced in low yield. The methodology is generally applicable to all proteins still on-resin and fully protected except for the terminal amino group. The protein is treated in order with the following steps: Biotinylation with NHS-biotin, HF cleavage, and avidin-agarose affinity chromatography. No special skills or automated equipment are needed to take advantage of this procedure.
Deibel
M R
MR
Upjohn Company, Kalamazoo, MI 49001.
Lobl
T J
TJ
Yem
A W
AW
D001360:Avidin; D001710:Biotin; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D007202:Indicators and Reagents; D010455:Peptides / Q000138:chemical synthesis / Q000302:isolation & purification*; D011506:Proteins / Q000138:chemical synthesis / Q000302:isolation & purification*
D007202:Indicators and Reagents; D010455:Peptides; D011506:Proteins; D001360:Avidin; D001710:Biotin