Binding of model immune complexes to erythrocyte CR1 facilitates immune complex uptake by U937 cells.
The E C3b/C4b receptor (CR1) has been shown to rapidly bind large complement-fixing immune complexes (IC) both in vivo and in vitro. It has been proposed that E (RBC) CR1 act as a shuttle mechanism, binding circulating IC and transporting them to tissue macrophages, thereby preventing their deposition in target tissues. In this study we have established an in vitro model system with which to study the transfer of model IC from CR1 on the RBC surface to phagocytic cells. Aggregated IgG (AHG) was opsonized with C3b, bound to RBC CR1, and the binding of these RBC-bound IC by a human monocyte cell line (U937 cells) was examined. U937 binding of AHG from the RBC surface was complete within 2 min, whereas binding of the same AHG from solution required 30 to 60 min. Despite the difference in kinetics of binding, the total amount of IC bound by U937 cells at equilibrium was the same for RBC-bound AHG and for AHG in solution. The transfer of AHG from the RBC to the U937 cell did not require exogenous factor I and was not accompanied by binding of RBC to U937 cells or by erythrophagocytosis. Our data lend support to the hypothesis that binding of IC to RBC CR1 may facilitate the clearance of IC from the circulation by enhancing their uptake by phagocytic cells.
Emlen
W
W
Division of Rheumatology, University of Colorado Health Sciences Center, Denver 80262.
Burdick
G
G
Carl
V
V
Lachmann
P J
PJ
D000888:Antibodies, Anti-Idiotypic / Q000378:metabolism; D000936:Antigen-Antibody Complex / Q000378:metabolism* / Q000502:physiology; D002460:Cell Line; D003179:Complement C3b / Q000378:metabolism*; D003168:Complement Fixation Tests; D004912:Erythrocytes / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D046911:Macromolecular Substances; D009000:Monocytes / Q000378:metabolism*; D009895:Opsonin Proteins; D011951:Receptors, Complement / Q000378:metabolism* / Q000502:physiology; D017463:Receptors, Complement 3b
D000888:Antibodies, Anti-Idiotypic; D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D046911:Macromolecular Substances; D009895:Opsonin Proteins; D011951:Receptors, Complement; D017463:Receptors, Complement 3b; D003179:Complement C3b