title: A negative regulatory element with properties similar to those of enhancers is contained within an Alu sequence.
abstract: A negative regulatory element has been found within a member of the African green monkey Alu family of interspersed repeated sequences. This "reducer" element decreased transcription in both directions from a cellular simian virus 40-like bidirectional promoter independently of both orientation and position. The reducer was not promoter specific since it also decreased expression from the simian virus 40 early and human beta-globin promoters. In addition, the reducer decreased transcription from a polymerase III promoter. The reducer was contained in 38 base pairs of an Alu family member and interacted specifically with a monkey cell nuclear protein.
authors.affiliation: Jackson Laboratory, Bar Harbor, Maine 04609.
authors.forename: J D
authors.initials: JD
authors.lastname: Saffer
authors.forename: S J
authors.initials: SJ
authors.lastname: Thurston
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops / Q000235:genetics; D004742:Enhancer Elements, Genetic; D005809:Genes, Regulator; D005822:Genetic Vectors; D005914:Globins / Q000235:genetics; D006801:Humans; D011401:Promoter Regions, Genetic; D015347:RNA Probes; D012091:Repetitive Sequences, Nucleic Acid; D013539:Simian virus 40 / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D015347:RNA Probes; D005914:Globins