Analysis of renal immune-deposits in canine leishmaniasis. Preliminary results.
Previous studies carried out on 34 dogs spontaneously infected by Leishmania infantum showed the presence of kidney lesions characterized by immunologically mediated glomerular and tubular damage. Glomerular immune-deposits were studied in 13 of these dogs. Immunoglobulins were isolated from kidney tissues by acid elution; IgG fractions from eluates, obtained by ammonium sulfate precipitation, were subjected to clonotypic analysis by autoradiography after isoelectrofocusing (IEF) using 125I radiolabelled goat IgG fraction-anti Fab2 of dog IgG. Idiotypic characterization of IgG eluted from kidney tissues was performed by IEF and autoradiography using both 125I radiolabelled membrane antigens of L. infantum extracted by Triton x 100 and 125I radiolabelled dog IgG for rheumatoid or anti-idiotypic activity. The IgG deposited in the kidney tissues of examined dogs were polyclonal and a specific activity against Leishmania membrane antigens was revealed. Meanwhile an anti-IgG activity of deposited immunoglobulins was not observed.
Mancianti
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F
Dipartimento di Patologia Animale, Università degli Studi di Pisa, Italy.
Poli
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A
Bionda
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D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis; D000936:Antigen-Antibody Complex / Q000032:analysis*; D000953:Antigens, Protozoan / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D004283:Dog Diseases / Q000276:immunology* / Q000473:pathology; D004285:Dogs / Q000276:immunology; D015432:Glomerulonephritis, Membranoproliferative / Q000209:etiology / Q000473:pathology / Q000662:veterinary*; D005923:Glomerulosclerosis, Focal Segmental / Q000209:etiology / Q000473:pathology / Q000662:veterinary*; D007105:Immune Complex Diseases / Q000276:immunology / Q000473:pathology / Q000662:veterinary; D007074:Immunoglobulin G / Q000276:immunology; D007678:Kidney Glomerulus / Q000276:immunology* / Q000473:pathology; D007893:Leishmania donovani / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000276:immunology / Q000473:pathology / Q000662:veterinary*
D000913:Antibodies, Protozoan; D000936:Antigen-Antibody Complex; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D007074:Immunoglobulin G