[Evaluation of a biotinylated probe for identification of Neisseria gonorrhoeae].
We evaluated a DNA biotinylated probe for the rapid identification of Neisseria gonorrhoeae in culture (Ortho Diagnostics System, Raritan, NJ). Twenty-one strains of N. gonorrhoeae, including type strain, 57 strains of other Neisseria species, and 104 strains of other genera were studied. The probe was highly sensitive (100%) and specific (96%). All N. gonorrhoeae strains gave strong signals, and only two cross-reaction were observed with N. lactamica, which has a close genetic relationship to N. gonorrhoeae. Our results indicate that specific recombinant DNA probe should offer a reliable and rapid method for routine diagnosis of Neisseria gonorrhoeae.
Bollet
C
C
Laboratoire de Microbiologie et d'Hygiène Hospitalière, Hpital Salvator, Sainte-Marguerite, MARSEILLE, France.
Grandguillot
M
M
Bouchemal
H
H
Boutefnouchet
N
N
de Micco
P
P
D001710:Biotin; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D005189:False Positive Reactions; D009344:Neisseria gonorrhoeae / Q000235:genetics / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization
D015342:DNA Probes; D004269:DNA, Bacterial; D001710:Biotin
Evaluation d'une sonde biotinylée pour l'identification de Neisseria gonorrhoeae.