Immunonephelometric determination of the apolipoprotein A-II.
A fully mechanized immunonephelometric method is described for the rapid and specific determination of apolipoprotein A-II in serum. The method utilizes commercially available sheep antiserum against human apolipoprotein A-II. Nephelometry was performed with the Behring Nephelometer Analyzer (BNA). A single determination can be performed in 12 minutes, requiring 10 microliters sample volume. The measuring range is about 0.08 to 1.25 g/l apolipoprotein A-II. Precision is characterized by intra-assay coefficients of variation of 3.37%, 3.93% and 4.49% for apolipoprotein A-II concentrations of 1.22 g/l, 0.376 g/l and 0.185 g/l, and inter-assay coefficients of variation of 4.27% for an apolipoprotein A-II concentration of 0.404 g/l, respectively. Accuracy of the method is shown by the close correlation of results with those from radial immunodiffusion (r = 0.913, y = 1.091 x -0.033, n = 75).
Hafner
G
G
Institut für Klinische Chemie, Johannes Gutenberg-Universität Mainz, FRG.
Luley
C
C
Prellwitz
W
W
D016633:Apolipoprotein A-II; D001054:Apolipoproteins A / Q000097:blood*; D005069:Evaluation Studies as Topic; D006801:Humans; D007158:Immunologic Techniques; D009391:Nephelometry and Turbidimetry / Q000379:methods*
D016633:Apolipoprotein A-II; D001054:Apolipoproteins A