title: Subcellular localization of superoxide dismutases, glutathione peroxidase and catalase in developing rat cerebral cortex.
abstract: The levels of copper- and zinc-containing superoxide dismutase (CuZnSOD), manganese-containing superoxide dismutase (MnSOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activity have been assessed in a nuclear fraction (NF), a mitochondrial fraction (MF) and a non-mitochondrial, non-nuclear fraction (NMNNF) isolated from developing rat cerebral cortex. The NF showed increasing CuZn and MnSOD activities and static, low activities of GSH-Px and CAT during development. The MF had increased levels of MnSOD and GSH-Px activities and a rapid decrease in CAT activity associated with development. Histochemical methods have localized greater CAT activity in mitochondria isolated from 2-day-old rat brain when compared to 77-day-old animals. Development was associated with increasing CuZnSOD activity, a decrease in CAT activity and, after an initial fall at 19 days, increasing GSH-Px activity in the NMNNF. Measurable activity of MnSOD were found in the NMNNF and appeared to be static during the time period assessed. A distinct ontogenetic pattern of oxidative enzyme activities and subcellular locations is associated with development in rat cerebral cortex.
authors.affiliation: Department of Clinical Neurological Sciences, Victoria Hospital, University of Western Ontario, London, Canada.
authors.forename: R
authors.initials: R
authors.lastname: Del Maestro
authors.forename: W
authors.initials: W
authors.lastname: McDonald
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D002374:Catalase / Q000032:analysis*; D002540:Cerebral Cortex / Q000201:enzymology*; D003576:Electron Transport Complex IV / Q000032:analysis; D005260:Female; D005979:Glutathione Peroxidase / Q000032:analysis*; D008297:Male; D008928:Mitochondria / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000201:enzymology; D013482:Superoxide Dismutase / Q000032:analysis*; D014018:Tissue Distribution
chemical_list: D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D003576:Electron Transport Complex IV