Chromosomal assignment of woodchuck hepatitis virus (WHV) DNA integration sites in a woodchuck hepatocellular carcinoma-derived cell line (WH257GE10).
The chromosomal sites of woodchuck hepatitis virus (WHV) DNA integration were identified in a woodchuck hepatocellular carcinoma-derived cell line (WH257GE10) by the in situ hybridization technique using 3H-labelled WHV whole genome (WHV 2) as a probe. The G-banded chromosome spreads from WH257GE10 were identified and diagrammed schematically according to their band patterns. WHV DNA was integrated into 2 sites: 33 region of the long arm of chromosome 6 (6q33) and 31 region of the long arm of chromosome 8 (8q31). Chromosomal sites of WHV DNA integration were stable during successive passage periods analyzed in vitro.
Mizuno
Y
Y
First Department of Internal Medicine, School of Medicine, Kanazawa University, Japan.
Murakami
S
S
Matsushita
F
F
Unoura
M
M
Kobayashi
K
K
Migita
S
S
Hattori
N
N
Ohno
S
S
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000209:etiology / Q000235:genetics* / Q000662:veterinary; D002460:Cell Line; D002871:Chromosome Banding; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D015342:DNA Probes; D004279:DNA, Viral / Q000235:genetics*; D005814:Genes, Viral; D006518:Hepatitis Viruses / Q000235:genetics*; D007621:Karyotyping; D007668:Kidney / Q000648:ultrastructure; D008113:Liver Neoplasms / Q000209:etiology / Q000235:genetics* / Q000662:veterinary; D008297:Male; D008392:Marmota / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D012589:Sciuridae / Q000382:microbiology*
D015342:DNA Probes; D004279:DNA, Viral