title: Evidence of endoplasmic reticulum-related Ca2+ ATPase in human microvascular endothelial cells.
abstract: We have demonstrated by immunological and molecular methods the presence of a reticulum endoplasmic-related Ca2+-ATPase in human omental microvascular endothelial cells (HOME cells). HOME cells reacted positively with a previously characterized sarcoplasmic reticulum Ca2+-ATPase antibody as demonstrated by indirect immunofluorescence. Western blotting revealed that the antibody recognized a 95-100 kDa protein. 35S-Metabolic labeling led to the detection of a similar protein with which the purified sarcoplasmic reticulum Ca2+-ATPase competed. Dot-blotting experiments indicated that a substantial amount of Ca2+-ATPase was present in HOME cell membranes. In addition, Northern blot analysis using a cDNA probe from cardiac sarcoplasmic reticulum showed the presence of mRNA species of 4-kb. As these experiments were conducted in comparison with cell types with well-defined Ca2+-ATPase in HOME cells.
authors.affiliation: Inserm U 150 and CNRS URA 184, Hôpital Lariboisière, Paris, France.
authors.forename: A
authors.initials: A
authors.lastname: Bikfalvi
authors.forename: J
authors.initials: J
authors.lastname: Enouf
authors.forename: R
authors.initials: R
authors.lastname: Bredoux
authors.forename: A
authors.initials: A
authors.lastname: Lompre
authors.forename: E
authors.initials: E
authors.lastname: Dupuy
authors.forename: N
authors.initials: N
authors.lastname: Bourdeau
authors.forename: S
authors.initials: S
authors.lastname: Levy-Toledano
authors.forename: G
authors.initials: G
authors.lastname: Tobelem
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000201:enzymology; D015152:Blotting, Northern; D015153:Blotting, Western; D000252:Calcium-Transporting ATPases / Q000235:genetics / Q000378:metabolism*; D015342:DNA Probes; D004721:Endoplasmic Reticulum / Q000201:enzymology*; D004730:Endothelium, Vascular / Q000201:enzymology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D011233:Precipitin Tests; D011817:Rabbits
chemical_list: D015342:DNA Probes; D000252:Calcium-Transporting ATPases