Neuronal activation regulates the expression of opioid receptors: possible role of glial-derived factors and voltage-dependent ion channels.
The previous observation that a continuous chemical depolarization of aggregating rat brain cells with KCl alters the expression of opioid receptors was examined in more detail. In contrast to its significant and converse effect on forebrain and hindbrain cells cultured in serum-containing medium, KCl had only a small and transient effect in serum-free cultures of both types. The basal receptor density in serum-free cultures was similar to the receptor density in KCl-treated serum-containing cultures, but medium conditioned by glial cells restored partially the effect of KCl in serum-free cultures. The effect of KCl in serum-containing forebrain cultures was enhanced by the voltage-dependent calcium channel blocker verapamil, and magnesium and cadmium had a similar, though smaller, effect. The sodium channel activator veratridine had a profound and dose-dependent inhibitory effect on the expression of the receptors in forebrain and hindbrain cultures, and tetrodotoxin blocked the veratridine effect. Information about the selectivity of the effect of neuronal activation on the various opioid receptor subtypes was obtained with the neuroblastoma X glioma hybrid M8 cells that possess only delta type opioid receptors. A Scatchard analysis of [3H]etorphine binding to these cells has shown that depolarization increased the Bmax, but had little, if any, effect on the affinity (KD) of the ligand to the receptors. The significance of depolarization and voltage-dependent sodium and calcium channels on the expression of different opioid receptor subtypes is discussed.
Simantov
R
R
Department of Genetics, Weizmann Institute of Science, Rehovot, Israel.
Levy
R
R
D000818:Animals; D001921:Brain / Q000166:cytology* / Q000378:metabolism; D015220:Calcium Channels / Q000502:physiology*; D002449:Cell Aggregation; D004174:Diprenorphine / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005048:Etorphine / Q000378:metabolism; D009474:Neurons / Q000502:physiology*; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D011957:Receptors, Opioid / Q000096:biosynthesis*; D015222:Sodium Channels / Q000502:physiology*; D013779:Tetrodotoxin / Q000494:pharmacology; D014701:Veratridine / Q000494:pharmacology
D015220:Calcium Channels; D011957:Receptors, Opioid; D015222:Sodium Channels; D004174:Diprenorphine; D005048:Etorphine; D013779:Tetrodotoxin; D011189:Potassium Chloride; D014701:Veratridine