Recombinant DNA probe for serotype-specific identification of bluetongue virus 17.
The double-stranded RNA genome from 117 field isolates of bluetongue virus (BTV) serotypes 10, 11, 13, and 17 was blotted onto nitrocellulose paper and hybridized with a radioactively labeled cloned copy of DNA genome segment 2 of BTV-17. Viral RNA from BTV prototype strains 2, 10, 11, 13, and 17 were used as controls. The probe hybridized only with the viral RNA from prototype BTV-17 virus and field isolates of BTV-17. There was no cross hybridization with field isolates of BTV serotypes 10, 11, and 13. A complementary DNA probe developed from genes coding for BTV serotype specificity was effectively used in a slot-blot hybridization system for efficiently characterizing the viral serotype.
de Mattos
C C
CC
Department of Veterinary Pathology, University of California, Davis 95616.
de Mattos
C A
CA
Osburn
B I
BI
Dangler
C A
CA
Chuang
R Y
RY
Doi
R H
RH
D000818:Animals; D001820:Bluetongue virus / Q000145:classification* / Q000235:genetics; D002417:Cattle; D015342:DNA Probes; D004274:DNA, Recombinant; D006041:Goats; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis* / Q000235:genetics; D012087:Reoviridae / Q000145:classification*; D012703:Serotyping; D012756:Sheep
D015342:DNA Probes; D004274:DNA, Recombinant; D012367:RNA, Viral