title: Quantitative determination of nitroglycerin by capillary gas chromatography-electron capture detection.
abstract: A rapid and sensitive capillary gas chromatographic method based on the one described by Noonan et al. [1] was used to evaluate the nitroglycerin content in serum samples of healthy volunteers, who had orally received a special preparation of the drug (Nisconitrine 6.5, Bio-Therabel). Concentrations were monitored up to 12 h after administration. In accordance with other literature data [2], no detectable amounts of the mother compound were found (limit of detection: 50 pg ml-1). Yet, significant amounts of the active metabolites, 1,2- and 1,3-dinitroglycerine could be demonstrated. Due to the low mass spectrometric response (electron impact ionization) of the different nitroglycerins, positive confirmation of the results with GC-MS was not possible. However, the concentrations reported here do agree with literature data [2], i.e. the ng ml-1 level.
authors.affiliation: Department of Pharmaceutical Sciences, University of Antwerp, Antwerp-Wilrijk, Belgium.
authors.forename: J J
authors.initials: JJ
authors.lastname: Janssens
authors.forename: M I
authors.initials: MI
authors.lastname: Selala
authors.forename: F F
authors.initials: FF
authors.lastname: Daelemans
authors.forename: S W
authors.initials: SW
authors.lastname: Andries
authors.forename: P J
authors.initials: PJ
authors.lastname: Schepens
mesh_terms: D002214:Capsules; D002849:Chromatography, Gas; D004583:Electrons; D006801:Humans; D007202:Indicators and Reagents; D005996:Nitroglycerin / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics
chemical_list: D002214:Capsules; D007202:Indicators and Reagents; D005996:Nitroglycerin