[Determination of guanine and cytosine contents of Trichophyton rubrum nuclear DNA with the thermal denaturation technique].
We report here on the G + C mol% content of T. rubrum DNA (including T. rubrum types I-V and an isolate from a patient with tinea pedis) as determined by a thermal denaturation technique. The results are as follows: 45.5, 57.37, 44.22, 45.38, 45.54, and 49.41 mol% for T. rubrum types I-V and isolate respectively. The results are similar to those in the literature, except that the value of T. rubrum type II is slightly higher. Our observations indicate that the morphological heterogeneity of different types of T. rubrum do not seem to be reflected in their G + C mol% values. The authors suggest that the method used here for determining G + C mol% is easy and reproducible.
Teng
C Y
CY
Wu
S X
SX
D003596:Cytosine / Q000032:analysis*; D004271:DNA, Fungal / Q000032:analysis*; D006147:Guanine / Q000032:analysis*; D006801:Humans; D009691:Nucleic Acid Denaturation; D014008:Tinea Pedis / Q000382:microbiology; D014249:Trichophyton / Q000032:analysis* / Q000302:isolation & purification
D004271:DNA, Fungal; D006147:Guanine; D003596:Cytosine