Glycosphingolipids in feces of germ-free rats as a source for studies of developmental changes of intestinal epithelial cell surface carbohydrates.
Non-acid and acid glycosphingolipids were isolated from feces of one litter of germ-free rats from day 17 to day 51. Quantitative and qualitative changes described for small intestine of conventional rats [Bouhours D, Bouhours J-F (1981) Biochem Biophys Res Commun 99:1384-89] were also found in the feces of these germ-free rats. A decrease in lactosylceramide and sialyllactosylceramide excretion and a change from N-acetylneuraminic acid to N-glycoloylneuraminic acid, as well as an appearance of type 1 chain blood group H-active penta- and decaglycosylceramides were observed during the weaning period. Thus the dramatic changes seen in rat intestinal glycosphingolipids postnatally seem to be primarily regulated by non-microbial factors.
Larson
G
G
Department of Clinical Chemistry, Gothenburg University, Sahlgren's Hospital, Sweden.
Midtvedt
T
T
D000017:ABO Blood-Group System; D000375:Aging / Q000378:metabolism*; D000818:Animals; D002855:Chromatography, Thin Layer; D004848:Epithelium / Q000502:physiology; D005243:Feces / Q000737:chemistry*; D005732:Gangliosides / Q000302:isolation & purification; D005856:Germ-Free Life; D006028:Glycosphingolipids / Q000737:chemistry / Q000302:isolation & purification*; D007421:Intestine, Small / Q000254:growth & development / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012794:Sialic Acids / Q000032:analysis
D000017:ABO Blood-Group System; D005732:Gangliosides; D006028:Glycosphingolipids; D012794:Sialic Acids