Experience with an enzyme immunoassay for serotyping human group A rotaviruses.
An enzyme immunoassay utilizing neutralizing monoclonal antibodies to VP7 of four human group A rotavirus serotypes successfully typed rotaviruses in 71.4% (568 of 796) of fecal specimens. Sensitivity was enhanced by using homologous capture and detector antibodies. Serotyping was most successful with specimens stored for less than 3 years and containing 10(4) or more particles per ml.
Unicomb
L E
LE
Department of Gastroenterology, Royal Children's Hospital, Parkville, Victoria, Australia.
Coulson
B S
BS
Bishop
R F
RF
D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D000956:Antigens, Viral; D002213:Capsid / Q000276:immunology; D036022:Capsid Proteins; D002648:Child; D003967:Diarrhea / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D006801:Humans; D007124:Immunoenzyme Techniques; D008854:Microscopy, Electron; D011237:Predictive Value of Tests; D011309:Preservation, Biological; D012401:Rotavirus / Q000145:classification* / Q000648:ultrastructure; D012400:Rotavirus Infections / Q000382:microbiology*; D012703:Serotyping; D013997:Time Factors; D014771:Virion / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D036022:Capsid Proteins; C050779:VP7 protein, Rotavirus