Interaction of monoclonal antiparathyroid antibody with Ca2+ agonistic actions of Mn2+ in normal human parathyroid cells.
Effects of the monoclonal antiparathyroid antibodies G11 and E11 on Mn2+ interaction with individual normal human parathyroid cells were studied. At 0.5mM Ca2+, 3mM Mn2+ induced a rapid transient increase in cytoplasmic Ca2+ [Ca2+i] followed by quenching of the fluorescence from the Ca2+ indicator fura-2 as Mn2+ entered into the cells. Whereas the antibody E11 had no effects, treatment with G11 abolished the Ca2+i transient and considerably delayed the entry of Mn2+. The results support the presence of a cation-sensitive receptor mechanism on parathyroid cells and indicate that the antibody G11 not only blocks the interaction between Ca2+ and this receptor mechanism but also that of Mn2+.
Johansson
H
H
Department of Surgery, University Hospital, Uppsala, Sweden.
Larsson
R
R
Juhlin
C
C
Rastad
J
J
Akerström
G
G
Gylfe
E
E
D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism*; D001572:Benzofurans; D002118:Calcium / Q000502:physiology*; D002121:Calcium Channel Blockers / Q000032:analysis / Q000502:physiology; D015220:Calcium Channels; D002462:Cell Membrane / Q000032:analysis / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D016257:Fura-2 / Q000031:analogs & derivatives*; D006801:Humans; D008345:Manganese / Q000378:metabolism* / Q000494:pharmacology; D010280:Parathyroid Glands / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D011978:Receptors, Nicotinic / Q000032:analysis / Q000502:physiology
D000911:Antibodies, Monoclonal; D001572:Benzofurans; D002121:Calcium Channel Blockers; D015220:Calcium Channels; D011978:Receptors, Nicotinic; C049925:fura-2-am; D008345:Manganese; D002118:Calcium; D016257:Fura-2