Studies of atopic patch tests.
35 patients were patch-tested for 72 h with house dust mite, timothy- and birch-pollen, Cladosporium herbarum and hen's egg white allergens, prepared in a cream in concentrations up to 1,000 times the concentration used for skin prick testing. 6 patients developed a total of 10 positive reactions. All the strong positive patch-test reactions occurred in patients with a strong positive skin prick test to the same allergen. Immunohistochemical studies of biopsied positive patch-test reactions demonstrated a mononuclear cell-infiltrate in the upper part of dermis, consisting mainly of T-cells, with a slight predominance of T-helper-cells as compared to T-suppressor cells, and about 10% CD1 positive cells. No significant responses were obtained in peripheral blood mononuclear cell-cultures stimulated with the various allergens. A positive patch-test reaction to birch-pollen was successfully transferred passively to a non-allergic-recipient, suggesting that the positive reaction may depend upon sensitizing factor(s) in the serum.
Langeland
T
T
Department of Dermatology, National Hospital, Rikshospitalet, Oslo, Norway.
Braathen
L B
LB
Borch
M
M
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000485:Allergens / Q000008:administration & dosage*; D000818:Animals; D000941:Antigens / Q000008:administration & dosage; D002648:Child; D003876:Dermatitis, Atopic / Q000276:immunology*; D004529:Egg White; D006801:Humans; D007116:Immunization, Passive; D007801:Langerhans Cells / Q000276:immunology; D008875:Middle Aged; D008925:Mites; D010328:Patch Tests / Q000379:methods*; D011058:Pollen; D012882:Skin Tests / Q000379:methods*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D000485:Allergens; D000941:Antigens