Characterization of a suppressive factor of platelet cytotoxic functions in human and rat schistosomiasis mansoni.
In a previous study we demonstrated that mitogen-stimulated CD8+ CD4-T cells from normal donors produce a suppressive lymphokine (PASL) of IgE-dependent platelet cytotoxicity. Here we demonstrate the production, after antigenic-stimulation, of this suppressive factor during ongoing infections by Schistosoma mansoni in man and in the rat. The T lymphocyte subpopulation producing this factor was also identified as expressing the marker of the suppressive subset. Because of the absence of species restriction, the relevance in vivo of PASL was determined in the rat model. In these conditions we observed a complete abolition of the protection normally conferred against a challenge infection by the passive transfer of platelets from immune to normal rats after treatment of transferred platelets with T lymphocyte supernatants.
Pancré
V
V
Centre d'Immunologie et de Biologie Parasitaire, Institut Pasteur, Lille, France.
Joseph
M
M
Capron
A
A
Delanoye
A
A
Vorng
H
H
Auriault
C
C
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D001665:Binding Sites; D001792:Blood Platelets / Q000276:immunology* / Q000378:metabolism; D001798:Blood Proteins / Q000096:biosynthesis / Q000378:metabolism / Q000502:physiology; D002474:Cell-Free System; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D006801:Humans; D007116:Immunization, Passive; D051381:Rats; D011916:Rats, Inbred F344; D012555:Schistosomiasis mansoni / Q000276:immunology*; D013491:Suppressor Factors, Immunologic / Q000096:biosynthesis / Q000378:metabolism / Q000502:physiology*; D013601:T-Lymphocytes / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D001798:Blood Proteins; D013491:Suppressor Factors, Immunologic; C052788:platelet inhibitory factor