Viral protein R of human immunodeficiency virus types 1 and 2 is dispensable for replication and cytopathogenicity in lymphoid cells.
Viral protein R (VPR) is conserved in human immunodeficiency virus types 1 and 2 (HIV-1 and HIV-2). To assess its function, we have constructed mutations within the vpr coding regions of HIV-1 and HIV-2 predicted to express truncated VPR products. Infectious virus was produced by each proviral clone and showed similar replication kinetics and cytopathogenicity when compared with the corresponding parental proviral clone.
Dedera
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Department of Medicine, Washington University, St. Louis, Missouri 63110.
Hu
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Vander Heyden
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Ratner
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L
D000818:Animals; D015139:Blotting, Southern; D002460:Cell Line; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D016336:Gene Products, vpr; D015497:HIV-1 / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D015498:HIV-2 / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D008214:Lymphocytes / Q000382:microbiology*; D009154:Mutation; D015183:Restriction Mapping; D012191:Retroviridae Proteins / Q000235:genetics / Q000502:physiology*; D014779:Virus Replication; D054325:vpr Gene Products, Human Immunodeficiency Virus
D004279:DNA, Viral; D016336:Gene Products, vpr; D012191:Retroviridae Proteins; D054325:vpr Gene Products, Human Immunodeficiency Virus