[Changes in the functional activity of polymorphonuclear leukocytes in pseudotubercular infection].
The functional activity of polymorphonuclear leukocytes (PNL) of the peripheral blood of rabbits infected orally with Y. pseudotuberculosis was studied. By day 5 of Y. pseudotuberculosis infection the pronounced stimulation of the myeloperoxidase system with the appearance of respiratory explosion was registered with the use of the nitro blue tetrazolium (NBT) test. The NBT-positive leukocyte index was found to increase 3.32-fold in comparison with the initial data. According to the results of the liposomal cationic test, the stimulation of the system of nonenzymatic cationic PNL proteins was revealed. By day 12 after infection the median cytochemical coefficient reached 2.035 +/- 0.005 conventional units, while in intact animals it was equal to 0.995 +/- 0.002 units. By day 20 a repeated, not so great, increase in the amount of NBT-positive PNL and in the content of cationic proteins in them was observed. The data thus obtained are indicative of the faintly pronounced secretory degranulation of PNL, which was probably one of the causes responsible for disturbances in the process of the digestion of Y. pseudotuberculosis by leukocytes, while their capacity for ingestion was retained.
Isachkova
L M
LM
Plekhova
N G
NG
D000818:Animals; D001798:Blood Proteins / Q000032:analysis; D006651:Histocytochemistry; D008247:Lysosomes / Q000032:analysis / Q000378:metabolism; D009504:Neutrophils / Q000032:analysis / Q000378:metabolism*; D009580:Nitroblue Tetrazolium; D009195:Peroxidase / Q000097:blood; D011817:Rabbits; D013997:Time Factors; D015009:Yersinia Infections / Q000097:blood*; D015012:Yersinia pseudotuberculosis Infections / Q000097:blood*
D001798:Blood Proteins; D009580:Nitroblue Tetrazolium; D009195:Peroxidase
Izmenenie funktsional'noĭ aktivnosti polimorfno-iadernykh leĭkotsito pri psevdotuberkuleznoĭ infektsii.