title: Amplification enhancers and replication origins in the autosomal chorion gene cluster of Drosophila.
abstract: Drosophila melanogaster follicle cells over-replicate the chromosomal domain containing the third chromosome chorion gene cluster. Multiple regions of this cluster are needed in cis for attainment of high levels of amplification. We have confirmed the importance of the proposed amplification control element (ACE3) and demonstrated that it can support low levels of follicular amplification in the absence of other elements, but that it lacks detectable activity as a DNA replication origin. We have also demonstrated the existence of additional amplification-enhancing regions (AERs), by analyzing the amplification levels of a series of in situ induced, nested deletions of the chorion cluster. These deletions were induced by P-transposase perturbation of a chorion transposon in a highly amplifying transformed line, and were not accompanied by re-transposition, making possible a quantitative analysis of amplification levels in the absence of chromosomal position effects. Analysis of endogenous replication intermediates in wild-type follicular DNA suggested that at least one of the AERs may be an origin of replication and that amplification uses at least one additional replication origin.
authors.affiliation: Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA 02138.
authors.forename: C
authors.initials: C
authors.lastname: Delidakis
authors.forename: F C
authors.initials: FC
authors.lastname: Kafatos
mesh_terms: D000818:Animals; D002823:Chorion / Q000378:metabolism; D002872:Chromosome Deletion; D004261:DNA Replication; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics*; D004742:Enhancer Elements, Genetic; D005260:Female; D005784:Gene Amplification; D008297:Male; D005810:Multigene Family; D015183:Restriction Mapping
chemical_list: D004251:DNA Transposable Elements