Molecular cloning of a feline leukemia provirus integrated adjacent to the c-myc gene in a feline T-cell leukemia cell line and the unique structure of its long terminal repeat.
This paper reports the molecular cloning of a rearranged c-myc region from the FT-1 cell line, which was derived from a spontaneous feline T-cell leukemia carrying the feline leukemia virus (FeLV). An abnormal c-myc EcoRI fragment of about 18 kilobases, detected by Southern blotting, was molecularly cloned from the DNA of the FT-1 cell line. The c-myc rearrangement in FT-1 was due to direct integration of the FeLV provirus genome immediately upstream of the c-myc gene in the opposite transcriptional orientation. Nucleotide sequencing showed that the LTR of this provirus had three copies of an enhancer-like sequence, unlike the sequences of FeLVs reported previously, which have only a single copy of this enhancer-like sequence.
Miura
T
T
Department of Animal Pathology, University of Tokyo, Japan.
Shibuya
M
M
Tsujimoto
H
H
Fukasawa
M
M
Hayami
M
M
D001483:Base Sequence; D002472:Cell Transformation, Viral; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics*; D005256:Leukemia Virus, Feline / Q000235:genetics*; D015458:Leukemia, T-Cell / Q000235:genetics / Q000382:microbiology / Q000662:veterinary*; D008969:Molecular Sequence Data; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D014407:Tumor Cells, Cultured
D004279:DNA, Viral; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc