Structure and expression of hcr, a locus rearranged with c-myc in a woodchuck hepatocellular carcinoma.
We have previously described a rearrangement of the proto-oncogene c-myc with a new cellular sequence of unknown function in a woodchuck primary liver tumor. We have now cloned and further analysed the normal woodchuck locus (termed hcr) of the sequence involved in the rearrangement with c-myc. The hcr locus is highly expressed in hepatocytes but not in other cell types examined and is conserved in mammals. Two unspliced hcr transcripts 4.5 and 4.7 kb long accumulate in liver cell nuclei. These transcripts differ only in their 3' extremities, located 180 bases apart, and by additional poly(A) tailing of the longer RNA species. The genomic sequence flanking the transcription start site contains variant elements of a classical eukaryotic promoter. Nucleotide sequence analysis of cDNA clones for the hcr RNA reveals that the 5' end of the hcr transcripts contains a short open reading frame of only 3 gamma codons initiated by an ATG. The biological function of her RNA remains to be determined.
Möröy
T
T
Unité de Recombinaison et Expression Génétique, INSERM U163, CNSR UA271, Institut Pasteur, Paris, France.
Etiemble
J
J
Bougueleret
L
L
Hadchouel
M
M
Tiollais
P
P
Buendia
M A
MA
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D004247:DNA; D004273:DNA, Neoplasm / Q000235:genetics*; D004720:Endonucleases; D005786:Gene Expression Regulation; D015321:Gene Rearrangement; D005796:Genes; D008114:Liver Neoplasms, Experimental / Q000235:genetics*; D008392:Marmota; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011519:Proto-Oncogenes; D012334:RNA, Neoplasm / Q000235:genetics; D015183:Restriction Mapping; D015719:Single-Strand Specific DNA and RNA Endonucleases
D003062:Codon; D004273:DNA, Neoplasm; D012334:RNA, Neoplasm; D004247:DNA; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases