Simultaneous determination of FUC and PGM1 by isoelectric focusing.
This paper describes a simple procedure for the simultaneous detection of FUC and PGM1 in semen, seminal stains, dental pulps and hair roots after separation by isoelectric focusing using Ampholine pH 5-7. The gel plate was first stained for FUC using an agarose overlay with a buffer of pH 7.5. After reading the FUC types, the FUC overlay was removed and replaced with an agarose overlay for PGM. The results show that the method is useful in medicolegal individualization of seminal stains and teeth when only small samples have to be examined.
Kido
A
A
Komatsu
N
N
Oya
M
M
Sekiyama
S
S
D003782:Dental Pulp / Q000032:analysis; D005554:Forensic Medicine; D006197:Hair / Q000032:analysis; D006801:Humans; D007525:Isoelectric Focusing; D008297:Male; D010733:Phosphoglucomutase / Q000302:isolation & purification*; D011110:Polymorphism, Genetic; D012661:Semen / Q000032:analysis; D005644:alpha-L-Fucosidase / Q000302:isolation & purification*
D005644:alpha-L-Fucosidase; D010733:Phosphoglucomutase