title: Slide culture sensitivity tests.
abstract: A new method for slide culture sensitivity tests of Mycobacterium tuberculosis is described in which smear-positive sputum spread on slides is incubated without prior decontamination in a selective lysed human blood medium. Results are available 7 days after setting up the tests and are particularly useful for guiding the treatment of smear-positive patients with a long history of unsuccessful chemotherapy. Drug concentrations and definitions of resistance are suggested for tests against isoniazid, streptomycin, PAS, rifampicin ethambutol and ethionamide. A good correlation was seen between the results of these tests and those of standard indirect sensitivity tests.
authors.affiliation: Department of Bacteriology, Royal Postgraduate Medical School, London.
authors.forename: J M
authors.initials: JM
authors.lastname: Dickinson
authors.forename: B W
authors.initials: BW
authors.lastname: Allen
authors.forename: D A
authors.initials: DA
authors.lastname: Mitchison
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology*; D001431:Bacteriological Techniques; D003470:Culture Media; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000254:growth & development; D013183:Sputum / Q000382:microbiology
chemical_list: D000995:Antitubercular Agents; D003470:Culture Media