Control elements situated downstream of the major transcriptional start site are sufficient for highly efficient polyomavirus late transcription.
In a transient expression assay in mouse fibroblasts in which neither replication nor T-antigen synthesis occurred, the polyomavirus late promoter functioned faithfully and even more efficiently than the simian virus 40 early promoter. Surprisingly, the DNA sequences upstream of the main transcriptional start sites were not required to obtain the high mRNA level observed. It appeared to result from the combined action of a basal promoter element within the A enhancer domain and of a more downstream element, located in the VP3 intron and abutting the late splice donor. We also show that although an enhancer region was required, enhancer function per se was not. Instead, it appeared that only a defined subset of the DNA-protein interactions necessary for enhancer function was involved in late promoter activity.
Bourachot
B
B
Unité des Virus Oncogènes, UA 1149 Centre National de la Recherche Scientifique, Institut Pasteur, Paris, France.
Yaniv
M
M
Herbomel
P
P
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D004742:Enhancer Elements, Genetic; D009154:Mutation; D010957:Plasmids; D011120:Polyomavirus / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis*; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014162:Transfection
D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral; D015500:Chloramphenicol O-Acetyltransferase