title: The yeast secretory pathway is perturbed by mutations in PMR1, a member of a Ca2+ ATPase family.
abstract: The genes for two new P-type ATPases, PMR1 and PMR2, have been identified in yeast. A comparison of the deduced sequences of the PMR proteins with other known ion pumps showed that both proteins are very similar to Ca2+ ATPases. PMR1 is identical to SSC1, a gene previously identified by its effect on secretion of some foreign proteins from yeast. Proteins secreted from pmr1 mutants lack the outer chain glycosylation that normally results from passage through the Golgi. Loss of PMR1 function suppresses the lethality of ypt1-1, a mutation that blocks the secretion pathway. These data suggest that PMR1 functions as a Ca2+ pump affecting transit through the secretory pathway.
authors.affiliation: Whitehead Institute for Biomedical Research Cambridge, Massachusetts 02142.
authors.forename: H K
authors.initials: HK
authors.lastname: Rudolph
authors.forename: A
authors.initials: A
authors.lastname: Antebi
authors.forename: G R
authors.initials: GR
authors.lastname: Fink
authors.forename: C M
authors.initials: CM
authors.lastname: Buckley
authors.forename: T E
authors.initials: TE
authors.lastname: Dorman
authors.forename: J
authors.initials: J
authors.lastname: LeVitre
authors.forename: L S
authors.initials: LS
authors.lastname: Davidow
authors.forename: J I
authors.initials: JI
authors.lastname: Mao
authors.forename: D T
authors.initials: DT
authors.lastname: Moir
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001693:Biological Transport, Active; D002118:Calcium / Q000502:physiology*; D000252:Calcium-Transporting ATPases / Q000235:genetics*; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000378:metabolism*; D005800:Genes, Fungal; D006031:Glycosylation; D008565:Membrane Proteins / Q000235:genetics* / Q000648:ultrastructure; D008969:Molecular Sequence Data; D009154:Mutation; D011487:Protein Conformation; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D008565:Membrane Proteins; D000252:Calcium-Transporting ATPases; D002118:Calcium