Similarities between the regulatory sequences of the unrelated tetracycline genes of pBR322 and Tn10.
The regulatory regions of the tetracycline genes present in pBR322 (pSC101) and in the transposon Tn10 are compared. They show a low degree of nucleotide sequence similarity but a high level of structure similarity. Furthermore, analyses of RNAs transcribed in the opposite direction of the pBR322 tet gene show that there are two mRNA initiation sites separated by 29 nucleotides. This suggests the existence of two promoters for the tet repressor gene in Tn10. These features reveal a strong resemblance of the mode of regulation between the tet operons of Tn10 and pSC101.
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Institut J. Monod, CNRS, Unité INSERM 257, Université Paris 7, France.
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D001483:Base Sequence; D004247:DNA / Q000235:genetics; D004251:DNA Transposable Elements; D015246:Deoxyribonuclease EcoRI; D004926:Escherichia coli / Q000235:genetics*; D008969:Molecular Sequence Data; D009876:Operon; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D013753:Tetracycline Resistance / Q000235:genetics*; D014158:Transcription, Genetic; D001618:beta-Lactamases / Q000235:genetics
D004251:DNA Transposable Elements; D012333:RNA, Messenger; D004247:DNA; D015246:Deoxyribonuclease EcoRI; D001618:beta-Lactamases