Routine determination of radiochemical purity of 99mTc-MIBI.
The manufacturer recommends that the radiochemical purity of 99mTc-MIBI be determined with alumina TLC plates developed in ethanol, but for routine use this is slow and requires care. After comparing a variety of systems, we obtained equivalent results using ITLC-SG strips developed with saline to determine [99mTc]pertechnetate and with acetone to determine 99mTc colloid; % 99mTc-MIBI was determined by difference from 100%. These separations are the opposite of those obtained with the routine polar 99mTc radiopharmaceuticals. Equivalent results were obtained even more rapidly by extracting 99mTc-MIBI from water into chloroform.
Proulx
A
A
Division of Nuclear Medicine, Ottawa Civic Hospital, Ontario, Canada.
Ballinger
J R
JR
Gulenchyn
K Y
KY
D002855:Chromatography, Thin Layer; D009570:Nitriles / Q000302:isolation & purification / Q000592:standards*; D009942:Organometallic Compounds / Q000302:isolation & purification / Q000592:standards*; D058428:Silica Gel; D012822:Silicon Dioxide; D017256:Technetium Tc 99m Sestamibi
D009570:Nitriles; D009942:Organometallic Compounds; D058428:Silica Gel; D012822:Silicon Dioxide; D017256:Technetium Tc 99m Sestamibi