The characterization of neutralizing and non-neutralizing monoclonal antibodies against equid herpesvirus type 1.
Seven monoclonal antibodies (MAbs) were produced which recognized equid herpesvirus type 1 (EHV-1). Three MAbs neutralized the subtype 1 virus (strain Army 183) in the presence of complement, but did not neutralize the subtype 2 virus (strain MD). All three MAbs immunoprecipitated an Mr 83K glycoprotein from a detergent-solubilized virion envelope preparation of the subtype 1 virus. The target antigens of the four non-neutralizing MAbs (6F11, 2A4, 1F10 and 8D9) were identified by immunoblotting against purified EHV-1 virions and had respective apparent Mr values of greater than 205K, greater than 205K, 97K and 13K.
Sinclair
R
R
Department of Infectious Diseases, Animal Health Trust, Newmarket, Suffolk, U.K.
Cook
R F
RF
Mumford
J A
JA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000276:immunology; D003165:Complement System Proteins / Q000276:immunology; D006564:Herpesviridae / Q000276:immunology*; D004861:Herpesvirus 1, Equid / Q000276:immunology*; D007114:Immunization; D007117:Immunization, Secondary; D007158:Immunologic Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D013997:Time Factors; D014759:Viral Envelope Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D014759:Viral Envelope Proteins; D003165:Complement System Proteins