Mapping in vivo topoisomerase I sites on simian virus 40 DNA: asymmetric distribution of sites on replicating molecules.
Complexes between simian virus 40 DNA and topoisomerase I (topo I) were isolated from infected cells treated with camptothecin. The topo I break sites were precisely mapped by primer extension from defined oligonucleotides. Of the 56 sites, 40 conform to the in vitro consensus sequence previously determined for topo I. The remaining 16 sites have an unknown origin and were detectable even in the absence of camptothecin. Only 11% of the potential break sites were actually broken in vivo. In the regions mapped, the pattern of break sites was asymmetric. Most notable are the clustering of sites near the terminus for DNA replication and the confinement of sites to the strand that is the template for discontinuous DNA synthesis. These asymmetries could reflect the role of topo I in simian virus 40 DNA replication and suggest that topo I action is coordinated spatially with that of the replication complex.
Porter
S E
SE
Department of Microbiology, School of Medicine, University of Washington, Seattle 98195.
Champoux
J J
JJ
D001483:Base Sequence; D001665:Binding Sites; D002166:Camptothecin; D002874:Chromosome Mapping; D004261:DNA Replication; D004264:DNA Topoisomerases, Type I / Q000235:genetics* / Q000378:metabolism; D004279:DNA, Viral / Q000235:genetics* / Q000378:metabolism; D005814:Genes, Viral; D013539:Simian virus 40 / Q000235:genetics* / Q000378:metabolism
D004279:DNA, Viral; D004264:DNA Topoisomerases, Type I; D002166:Camptothecin