Interactions between structure transitions in a torsionally constrained DNA.
We used S1 nuclease cleavage in conjunction with gel electrophoresis to evaluate torsion-induced cruciform extrusion at two inverted repeat sequences, IRS-B and IRS-C of plasmid pUC12. These structure transitions affect each other through competition for the available torsional free energy according to their relative energies of activation and the magnitude of DNA duplex unwinding associated with each transition. They can be modulated by the level of DNA negative torsion. Interplays between transition sequences occur over long distances and are independent of relative orientation of transition sites. DNA binding factors that enhance or repress structural transitions of specific sequences may, thus, regulate the structural and functional properties of torsionally coupled, distal sequences.
Wang
Y
Y
Institute of Human Genetics, University of Minnesota, Minneapolis/St. Paul, MN 55108.
Sauerbier
W
W
Blumenfeld
M
M
D001483:Base Sequence; D004247:DNA; D004587:Electrophoresis, Agar Gel; D004720:Endonucleases; D006358:Hot Temperature; D009690:Nucleic Acid Conformation; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013816:Thermodynamics
D004247:DNA; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases