Cell wall constituents of Microcystis sp. PCC 7806.
Cell walls of Microcystis sp. PCC 7806 were purified from cell homogenates by sucrose density centrifugation and Triton X-100 extraction. The outer membrane contained carotenoids, two major peptidoglycan-associated proteins (Mr 49,000 and 52,000), and lipopolysaccharide (LPS) as indicated by the presence of 3-hydroxy fatty acids (3-OH-14:0, 3-OH-16:0, 3-OH-18:0), 4-oxo-18:0 fatty acid, and GlcN as lipid A components in addition to rare O-methyl sugars (2-O-methyl-6-deoxyhexoses I and II). The peptidoglycan (A1 gamma-type) was found to be covalently linked to a wall polysaccharide composed of GlcN, ManN, Man, Glc, and phosphate.
Jürgens
U J
UJ
Institut für Biologie II, Mikrobiologie, Albert-Ludwigs-Universität, Freiburg, F.R.G.
Martin
C
C
Weckesser
J
J
D002338:Carotenoids / Q000032:analysis; D002473:Cell Wall / Q000032:analysis; D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D006858:Hydrofluoric Acid; D008070:Lipopolysaccharides / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis*; D046931:Microcystis / Q000032:analysis / Q000648:ultrastructure*; D010457:Peptidoglycan / Q000032:analysis*; D011134:Polysaccharides / Q000032:analysis*
D008070:Lipopolysaccharides; D008565:Membrane Proteins; D010457:Peptidoglycan; D011134:Polysaccharides; D002338:Carotenoids; D006858:Hydrofluoric Acid