title: Liquid chromatographic determination of aflatoxins, ochratoxin A, and zearalenone in grains, oilseeds, and animal feeds by post-column derivatization and on-line sample cleanup.
abstract: A liquid chromatographic method using on-line sample cleanup, reverse flow analytical column loading, gradient elution, and postcolumn derivatization with iodine permits direct, rapid determination of aflatoxins B1, B2, G1, and G2, as well as ochratoxin A and zearalenone. Limits of quantitation are 5 ppb for the aflatoxins and ochratoxin A and 30 ppb for zearalenone. This procedure performs well as a multimycotoxin screen for cereal grains and oilseeds, with more limited success in complete animal feeds.
authors.affiliation: Robert S. Kerr Laboratories, Ada, OK 74820.
authors.forename: N
authors.initials: N
authors.lastname: Chamkasem
authors.forename: W Y
authors.initials: WY
authors.lastname: Cobb
authors.forename: G W
authors.initials: GW
authors.lastname: Latimer
authors.forename: C
authors.initials: C
authors.lastname: Salinas
authors.forename: B A
authors.initials: BA
authors.lastname: Clement
mesh_terms: D000348:Aflatoxins / Q000032:analysis*; D000821:Animal Feed / Q000032:analysis*; D010367:Arachis / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003369:Cottonseed Oil / Q000032:analysis*; D002523:Edible Grain / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis; D009793:Ochratoxins / Q000032:analysis*; D012118:Resorcinols / Q000032:analysis*; D003313:Zea mays / Q000032:analysis; D015025:Zearalenone / Q000032:analysis*
chemical_list: D000348:Aflatoxins; D003369:Cottonseed Oil; D009793:Ochratoxins; D012118:Resorcinols; C025589:ochratoxin A; D015025:Zearalenone