Identification of the alpha 3-subunit in the GABAA receptor purified from bovine brain.
Polyclonal antibodies have been raised to synthetic amino acid sequences of the bovine GABAA receptor alpha 1 and alpha 3 subunits. Anti-alpha 1 subunit antibodies recognise a polypeptide of 53 kDa whereas anti-alpha 3 subunit antibodies recognise a polypeptide of 59-60 kDa, in Western blots of GABAA receptor purified from adult bovine cerebral cortex, cerebellum and 12-day calf cerebral cortex.
Stephenson
F A
FA
MRC Molecular Neurobiology Unit, MRC Centre, Cambridge, England.
Duggan
M J
MJ
Casalotti
S O
SO
D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism*; D002417:Cattle; D002531:Cerebellum / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D007120:Immunochemistry; D008970:Molecular Weight; D010446:Peptide Fragments / Q000302:isolation & purification; D011963:Receptors, GABA-A / Q000302:isolation & purification*
D010446:Peptide Fragments; D011963:Receptors, GABA-A