Evaluation of four methods for cytomegalovirus antibody detection for use by a bone marrow transplantation service.
Four methods, latex agglutination, indirect fluorescent antibody, enzyme immunoassay, and complement fixation, were compared for cytomegalovirus antibody screening and for pre- and posttransplant determinations on bone marrow transplant recipients. Latex agglutination was most sensitive (98%) and specific (97%) for screening and pretransplant determinations and was quickest and easiest to perform. In posttransplant sera from allogeneic bone marrow transplant recipients, all methods except complement fixation detected cytomegalovirus antibody from therapeutically administered globulin preparations; this made it difficult to determine the significance of changes in cytomegalovirus antibody titer.
Leland
D S
DS
Department of Pathology, Indiana University Hospitals, Indianapolis 46223.
Barth
K A
KA
Cunningham
E B
EB
Jansen
J
J
Tricot
G J
GJ
French
M L
ML
D000914:Antibodies, Viral / Q000032:analysis*; D016026:Bone Marrow Transplantation; D003168:Complement Fixation Tests; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D007841:Latex Fixation Tests; D011237:Predictive Value of Tests
D000914:Antibodies, Viral