Interactions between receptors for interleukin 2 and interleukin 4 on lines of helper T cells (HT-2) and B lymphoma cells (BCL1).
IL-2 and IL-4 induce a synergistic proliferative response in HT-2 cells, suggesting that IL-2Rs and IL-4Rs may interact. The purpose of this study was to examine the effect of IL-4 on the expression and function of IL-2Rs. Preincubation of HT-2 and BCL1-3B3 cells with IL-4 for 60 min at 4 degrees C or 37 degrees C resulted in a partial decrease in the number, but not the affinity of high affinity IL-2Rs as evidenced by Scatchard analysis of binding data. The decrease in the number of high affinity receptors correlated with decreased internalization of IL-2. After preincubation with IL-4, crosslinking of 125I-IL-2 to high affinity IL-2Rs also demonstrated a approximately 50% reduction in the number of high affinity IL-2Rs. Another lymphokine, IL-1, which acts on HT-2 cells, had no measurable effect on the affinity or number of IL-2Rs. Taken together, these results indicate that IL-4 downregulates the expression of high affinity IL-2Rs on some cells. It is not known whether this occurs by a direct ligand-mediated receptor interaction, by the sharing of a common receptor subunit, or by interaction of the two receptors with another membrane molecule or cytoskeletal component.
Fernandez-Botran
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R
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235.
Sanders
V M
VM
Vitetta
E S
ES
D001402:B-Lymphocytes / Q000502:physiology*; D004357:Drug Synergism; D004705:Endocytosis; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism / Q000494:pharmacology; D015847:Interleukin-4; D007378:Interleukins / Q000378:metabolism / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D046911:Macromolecular Substances; D008970:Molecular Weight; D015375:Receptors, Interleukin-2 / Q000502:physiology*; D019948:Receptors, Interleukin-4; D011975:Receptors, Mitogen / Q000502:physiology*; D006377:T-Lymphocytes, Helper-Inducer / Q000502:physiology*; D014407:Tumor Cells, Cultured
D007376:Interleukin-2; D007378:Interleukins; D046911:Macromolecular Substances; D015375:Receptors, Interleukin-2; D019948:Receptors, Interleukin-4; D011975:Receptors, Mitogen; D015847:Interleukin-4