title: Activated peripheral blood mononuclear cells a possible substitute to B-lymphocytes in HLA-DR determination.
abstract: The serological determination of class II antigens is still a mandatory test prior to allotransplantation. It is known that these antigens are normally expressed on B-lymphocytes and monocytes. The B-lymphocytes that constitute 10-15% of total blood lymphocytes are the cells currently used for HLA-DR typing. To avoid HLA-DR typing difficulties, or even impossibilities that are frequently encountered among some patient groups, we studied the response of peripheral blood mononuclear cells (PBMNC)--as an alternative source of cells for class II antigen typing--to in vitro mitogen and interleukin-2 activation and propagation. Although the patients included in this study were selected having pre-known HLA-DR typing difficulties, all of them could be adequately typed by this method.
authors.forename: M A
authors.initials: MA
authors.lastname: el-Barrawy
mesh_terms: D001402:B-Lymphocytes; D006684:HLA-DR Antigens / Q000302:isolation & purification*; D006650:Histocompatibility Testing; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008213:Lymphocyte Activation
chemical_list: D006684:HLA-DR Antigens