Chromosome 1 deletions in human neuroblastomas: generation and fine mapping of microclones from the distal 1p region.
Human neuroblastomas show a high incidence of deletions in the distal region of the short arm of chromosome 1. In pursuit of a molecular analysis of these deletions, we have generated a microclone bank from microdissected 1p35-pter chromosomal fragments. To allow a rapid localization of the microclones, we have also generated a panel of (human x mouse) hybrid cell lines through microcell-mediated chromosome transfer. The hybrid cells contained different portions of the human chromosome 1 on a murine background. A total of 20 randomly chosen single or low-copy microclones were localized by Southern analysis on DNA of the hybrid panel: All probes were derived from chromosome I. Sixteen mapped in region 1p36.1-pter, two in 1p22-p36.1, and another two in 1cen-qter. The mapping of ten of these microclones was further refined by in situ hybridization. Cells of the neuroblastoma line GI-ME-N carry two types of chromosome 1, one cytogenetically normal and another with a translocation reported to be in 1p36.2, i.e., a t(1;?) (p36.2;?) marker. Using cell hybridization, we separated the two chromosome 1 types of GI-ME-N into different hybrid cell clones. Southern hybridization of three microclones from distal Ip to DNA of the hybrid cell clones revealed that the breakpoint in the translocated chromosome I was located in 1p36.1.
Martinsson
T
T
Institut für Experimentelle Pathologie, Deutsches Krebsforschungszentrum, Heidelberg, Federal Republic of Germany.
Weith
A
A
Cziepluch
C
C
Schwab
M
M
D010582:Bacteriophage lambda; D015139:Blotting, Southern; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D003001:Cloning, Molecular / Q000379:methods; D004273:DNA, Neoplasm / Q000235:genetics; D004210:Dissection / Q000379:methods; D005822:Genetic Vectors; D006801:Humans; D006822:Hybrid Cells; D007621:Karyotyping; D008677:Metaphase / Q000235:genetics; D008979:Moloney murine leukemia virus; D009447:Neuroblastoma / Q000235:genetics*; D009693:Nucleic Acid Hybridization
D004273:DNA, Neoplasm