Simple and rapid purification of F1-ATPase from mitochondria.
A simple, rapid method for isolation of F1-ATPase from bovine heart mitochondria using the cation exchange resin Dynospheres PD-201-SCX was developed. The method is based on the fact that hydrophobic membrane proteins are adsorbed efficiently on this resin, whereas hydrophilic proteins, such as F1-ATPase, are not. By this method F1-ATPase of high purity and enzyme activity can be obtained from mitochondria in about 2 h.
Shinohara
Y
Y
Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
Nakagawa
M
M
Terada
H
H
D000818:Animals; D002417:Cattle; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D008565:Membrane Proteins / Q000302:isolation & purification; D008929:Mitochondria, Heart / Q000201:enzymology*; D010085:Oxidative Phosphorylation; D006180:Proton-Translocating ATPases / Q000302:isolation & purification* / Q000378:metabolism; D012116:Resins, Plant; D013997:Time Factors
D008565:Membrane Proteins; D012116:Resins, Plant; D006180:Proton-Translocating ATPases