title: Isolation of a gene that down-regulates nitrate assimilation and influences another regulatory gene in the same system.
abstract: Glutamine is the preferred source of nitrogen of Neurospora crassa. In its presence and that of the gene product of MS5 (nmr-1), the fungus represses the assimilation of less preferred forms of nitrogen, such as nitrate. In the absence of glutamine and the presence of the product of gene nit-2, less preferred forms of nitrogen are assimilated as long as a specific pathway for their assimilation is induced. We report here the isolation, from a cosmid bank, of a gene that complements MS5 and can also complement nit-2. We speculate that this result suggests an interaction between the MS5 and nit-2 gene products and that this is important in the regulation of nitrate assimilation.
authors.affiliation: Department of Biology, McMaster University, Hamilton, Ontario, Canada.
authors.forename: G J
authors.initials: GJ
authors.lastname: Sorger
authors.forename: D
authors.initials: D
authors.lastname: Brown
authors.forename: M
authors.initials: M
authors.lastname: Farzannejad
authors.forename: A
authors.initials: A
authors.lastname: Guerra
authors.forename: M
authors.initials: M
authors.lastname: Jonathan
authors.forename: S
authors.initials: S
authors.lastname: Knight
authors.forename: R
authors.initials: R
authors.lastname: Sharda
mesh_terms: D003360:Cosmids; D005786:Gene Expression Regulation; D005800:Genes, Fungal; D005809:Genes, Regulator; D005816:Genetic Complementation Test; D005973:Glutamine / Q000378:metabolism; D009154:Mutation; D009492:Neurospora crassa / Q000235:genetics / Q000378:metabolism; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000235:genetics / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism*; D010957:Plasmids; D015183:Restriction Mapping; D014170:Transformation, Genetic
chemical_list: D009566:Nitrates; D005973:Glutamine; D009565:Nitrate Reductases; D050901:Nitrate Reductase