The mode of inhibition of the Na+-K+ pump activity in mast cells by calcium.
1 The inhibition by calcium of the Na(+)-K+ pump in the plasma membrane of rat peritoneal mast cells was studied in pure populations of the cells by measuring the ouabain-sensitive uptake of the radioactive potassium analogue, 86rubidium (86Rb+). 2 Exposure of the cells to calcium induced a time- and concentration-dependent decrease in the ouabain-sensitive K+(86Rb+)-uptake of the cells without influencing the ouabain-resistant uptake. The development of the inhibition required the presence of potassium in the medium in the millimolar range (1.5-8.0 mM), and it did not occur at a concentration of potassium (0.24 mM) that is probably rate limiting for the pump activity. In the presence of 1 mM calcium full inhibition developed almost immediately and was not readily reversed. The inhibition was not significantly reduced by 15 min incubation with 1.2 mM EGTA. 3 The inhibitory action of calcium did not develop when the mast cells were incubated in a potassium-free medium, which is known to block Na(+)-K+ pump activity and allow accumulation of sodium inside the cells. Likewise, increasing the sodium permeability of the plasma membrane by monensin abolished the inhibition of the pump activity. In both cases, incubation of the cells with 4.7 mM potassium and tracer amounts of 86Rb+ resulted in a very large uptake of K+ (86Rb+) into the cells (up to 2 nmol per 10(6) cells min-1), indicating a high activity of the Na(+)-K+ pump. 4. These observations support the view that long-term incubation of rat peritoneal mast cells in a calcium-free medium increases the permeability of the plasma membrane to sodium, and the consequent increase in the intracellular concentration of sodium causes an increase in the activity of the pump. Addition of calcium to the cell suspension decreases the sodium permeability, and hence the pump activity. This hypothesis is supported by the stimulation of pump activity produced by monensin, which is not inhibited by calcium. The enhancement of pump activity after exposure of calcium-deprived cells to EGTA might be the result of a further increase in the sodium permeability of the plasma membrane.
Knudsen
T
T
Department of Pharmacology, Medical School, Odense University, Denmark.
Johansen
T
T
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002118:Calcium / Q000494:pharmacology*; D002463:Cell Membrane Permeability / Q000187:drug effects; D004533:Egtazic Acid / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D008985:Monensin / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012414:Rubidium Radioisotopes; D012964:Sodium / Q000378:metabolism*
D012414:Rubidium Radioisotopes; D004533:Egtazic Acid; D008985:Monensin; D012964:Sodium; D011188:Potassium; D002118:Calcium