Surface labeling of Pneumocystis carinii from in vitro culture.
Pneumocystis carinii is an opportunistic pathogen of man, carried as a commensal in healthy subjects. It frequently causes a fatal pneumonia in the immunosuppressed host. It is a major complication of HIV-1 infection in man (AIDS). Using surface radioiodination of rat-derived P. carinii trophozoites obtained from in vitro culture, a major surface glycoprotein (gp120) has been identified. The glycoprotein exhibits adherent behavior similar to that of the intact organism. Purification of gp120 by conventional methods was unsuccessful as the glycoprotein irreversibly bound to numerous column matrices. A combination of gel chromatography and hydroxyapatite chromatography in sodium dodecylsulfate was utilized to purify the glycoprotein. Some preliminary characterization of the glycoprotein is presented.
Radding
J A
JA
Yale MacArthur Center for Molecular Parasitology, Yale University School of Medicine, New Haven, CT 06510.
Armstrong
M Y
MY
Bogucki
M S
MS
Richards
F F
FF
D000818:Animals; D001345:Autoradiography; D055598:Chemical Phenomena; D002621:Chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D007457:Iodine Radioisotopes; D008358:Mannose / Q000032:analysis; D008562:Membrane Glycoproteins / Q000032:analysis*; D008698:Mesylates; D008970:Molecular Weight; D011010:Pneumocystis / Q000032:analysis*
D007457:Iodine Radioisotopes; D008562:Membrane Glycoproteins; D008698:Mesylates; C012077:trifluoromethanesulfonic acid; D008358:Mannose