Chromatin structure and levels of expression and DNA methylation in the E3 region of chromosomally integrated adenovirus type 12 DNA.
Integrated adenovirus type 12 (Ad12) genes in Ad12-transformed cell lines were investigated for chromatin structure, expression levels and states of DNA methylation. The E3 region in the Ad12-transformed cell line HA12/7 is hypermethylated and not expressed. The same region in the Ad12-transformed hamster cell lines T637 and A2497-3 is transcribed and undermethylated (Kruczek, I. and Doerfler, W. (1982) EMBO J. 1, 409-414). There was no significant difference in the DNase I sensitivity of the E3 region when nuclei of the aforementioned cell lines were incubated with this nuclease. In contrast, incubation of these nuclei with the restriction endonuclease PstI and subsequent cleavage of the DNA with BamHI generated an additional 0.9 kbp fragment in T637 and A2497-3 DNA which was not observed after treating HA12/7 nuclei and DNA in the same way. This finding was interpreted as indicative of differences in the chromatin structure of the E3 region depending on its state of transcriptional activity and its level of methylation. The E1 and major late promoter regions, which were transcriptionally active and inactive, respectively, in all three cell lines investigated, did not exhibit differences in sensitivity towards DNase I or PstI treatment of nuclei. More refined technology will be required to compare the chromatin structure of active versus inactive genes.
Bartnik
E
E
Institute of Genetics, University of Cologne, F.R.G.
Doerfler
W
W
D017456:Adenovirus Early Proteins; D000260:Adenoviruses, Human / Q000235:genetics*; D015139:Blotting, Southern; D002472:Cell Transformation, Viral; D002843:Chromatin / Q000502:physiology / Q000648:ultrastructure*; D004279:DNA, Viral / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000494:pharmacology; D005786:Gene Expression Regulation; D005814:Genes, Viral; D008745:Methylation; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping
D017456:Adenovirus Early Proteins; D002843:Chromatin; D004279:DNA, Viral; D009856:Oncogene Proteins, Viral; D003850:Deoxyribonuclease I