title: Glucosyltransferase activity in mitochondria. Biosynthesis of glucosyl-phosphoryl-dolichol in inner mitochondrial membranes.
abstract: 1. Inner mitochondrial membranes are able to transfer [14C]glucose from UDP-[14C]glucose onto dolichylmonophosphate. 2. Synthesis of dolichyl-phosphoryl-glucose takes place only in the presence of exogenous dolichyl-monophosphate loaded into phospholipid vesicles. 3. Neutral phospholipids interact preferentially with the membrane-bound enzyme. The effect of phospholipids is not related to the length of fatty acid chains but a correlation between the activation and the degree of unsaturation of fatty acid chains has been found. 4. This enzyme required divalent cations for activity. Such a requirement might be related to lipid-protein interactions which favour a suitable conformation of glycosyltransferase.
authors.affiliation: Department of Biochemistry, University of Lyon, Lyon-Sud Medical School, INSERM CNRS U. 189, Oullins, France.
authors.forename: D
authors.initials: D
authors.lastname: Ardail
authors.forename: F
authors.initials: F
authors.lastname: Lerme
authors.forename: O
authors.initials: O
authors.lastname: Gateau-Roesch
authors.forename: P
authors.initials: P
authors.lastname: Louisot
authors.forename: R
authors.initials: R
authors.lastname: Morelis
mesh_terms: D000818:Animals; D002413:Cations, Divalent; D005231:Fatty Acids, Unsaturated / Q000502:physiology; D005947:Glucose / Q000378:metabolism; D005964:Glucosyltransferases / Q000378:metabolism*; D007425:Intracellular Membranes / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D008930:Mitochondria, Liver / Q000201:enzymology* / Q000378:metabolism; D010743:Phospholipids / Q000502:physiology; D011103:Polyisoprenyl Phosphate Monosaccharides / Q000096:biosynthesis*; D011105:Polyisoprenyl Phosphate Sugars / Q000096:biosynthesis*; D011786:Quality Control
chemical_list: D002413:Cations, Divalent; D005231:Fatty Acids, Unsaturated; D010743:Phospholipids; D011103:Polyisoprenyl Phosphate Monosaccharides; D011105:Polyisoprenyl Phosphate Sugars; C002968:dolichol-D-glucosylmonophosphate; D005964:Glucosyltransferases; D005947:Glucose