[Production of monoclonal antibodies for Mycobacterium tuberculosis Aoyama-B and immunological activity of the affinity-purified antigens].
Nine hybridomas of BALB/c mouse, producing monoclonal antibodies (MAb: MTA 1-9) directed to Mycobacterium tuberculosis AOYAMA-B (M. tbc AB), were established. The MAbs reacted to M. tbc AB antigens, forming a main band of 32 KD, 24 KD or 19 KD on immunoblotting, except MTA 1 that reacted with 58 KD in addition to 32 KD antigen. Immunoglobulin isotypes of MAbs were IgM, IgG 1 or IgG 2b. On a mode of reactivity of ELISA against various mycobacterial antigens, MAbs were divided into two categories: those reacting to human type mycobacterial antigens alone and those reacting both to the human and non-human type. The MAbs could further be classified into 4 groups by subtle discrimination of ELISA reactivity to the antigens. Three kind of MAb-affinity-purified antigens (MAb-Ag) from M. tbc AB were obtained. Immunogenic activities of the three MAb-Ags, together with that of control PPDs were examined. Every MAb-Ag showed positive reaction in ELISA, DTH skin test and [3H]TdR incorporation of lymphnode cells, on M. tbc AB sensitized rabbit, guinea pig and rat, respectively, with almost equal level of those of PPDs. However, in some occasions, MAb-Ags positively reacted in animals primed by non-human type mycobacteria. It was discussed that whether a homogeneous antigen like MAb-Ag, bearing limited number of epitopes, could be as immunogenic as PPDs consisting of multi-components.
Tsuyuguchi
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D000818:Animals; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000276:immunology*; D002846:Chromatography, Affinity; D006168:Guinea Pigs; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D011817:Rabbits; D051381:Rats
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial