title: Production of antibodies against the coenzyme pyrrolequinoline quinone.
abstract: Polyclonal antibodies against pyrrolequinoline quinone have been elicited in rabbits. These antibodies react with free and protein-bound pyrrolequinoline quinone. In particular they react with native and denatured lentil seedling amine oxidase as detected by dot-blot and ELISA assays. The presence of 1 mol pyrrolequinoline quinone per mol of enzyme was determined by the last method.
authors.affiliation: Istituto Regina Elena per la Ricerca sul Cancro, Roma, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Citro
authors.forename: A
authors.initials: A
authors.lastname: Verdina
authors.forename: R
authors.initials: R
authors.lastname: Galati
authors.forename: G
authors.initials: G
authors.lastname: Floris
authors.forename: S
authors.initials: S
authors.lastname: Sabatini
authors.forename: A
authors.initials: A
authors.lastname: Finazzi-Agrò
mesh_terms: D006631:Amine Oxidase (Copper-Containing); D000906:Antibodies / Q000276:immunology; D000941:Antigens / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D007887:Fabaceae; D006358:Hot Temperature; D015151:Immunoblotting; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000032:analysis*; D045542:PQQ Cofactor; D010944:Plants / Q000201:enzymology*; D010946:Plants, Medicinal; D011489:Protein Denaturation; D015363:Quinolones / Q000032:analysis* / Q000276:immunology
chemical_list: D000906:Antibodies; D000941:Antigens; D015363:Quinolones; D045542:PQQ Cofactor; D006631:Amine Oxidase (Copper-Containing); D000587:Oxidoreductases Acting on CH-NH Group Donors