title: Improvement of the reproducibility of ion-pair HPLC of 99mTc(Sn)EHDP complexes and the influence of the Sn(II) concentration on the composition of the reaction mixture.
abstract: The use of a non-volatile modifier and the simultaneous exclusion of oxygen by flushing with helium in ion-pair HPLC of 99mTc(Sn)EHDP-complexes prevents spurious oxidation during the separation process. It thus enables the omission of the reducing agent from the eluent solution and the determination of the labelling percentage from the TcO4(1)-peak. In addition, retention times become more reproducible.
authors.affiliation: Netherlands Energy Research Foundation, Petten.
authors.forename: R J
authors.initials: RJ
authors.lastname: Nieuwland
authors.forename: H A
authors.initials: HA
authors.lastname: Das
authors.forename: C L
authors.initials: CL
authors.lastname: de Ligny
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D012968:Etidronic Acid / Q000302:isolation & purification*; D009942:Organometallic Compounds / Q000302:isolation & purification*; D015609:Organotechnetium Compounds; D013667:Technetium / Q000302:isolation & purification*; D014001:Tin
chemical_list: D009942:Organometallic Compounds; D015609:Organotechnetium Compounds; C036811:technetium Tc 99m (Sn) etidronate; C019561:technetium Tc 99m etidronate; D013667:Technetium; D014001:Tin; D012968:Etidronic Acid