title: Synthesis of the pro-peptide of subtilisin BPN'.
abstract: Subtilisin, a bacterial serine protease, is secreted as pre-pro-subtilisin. Previously, we demonstrated that the pro-peptide moiety of intact pro-subtilisin can guide the folding of inactive protein to active enzyme both in an intramolecular (6) and intermolecular manner (18). Herein is reported the total chemical synthesis of the pro-sequence (77 amino acids) of pre-pro-subtilisin BPN' carried out by solid phase methods. The structure was confirmed by both sequencing and amino acid analysis of the fragment peptides resulting from a V-8 protease digest. Preliminary studies indicate that the synthetic pro-peptide itself can renature denatured subtilisin BPN'. This study demonstrates a novel method for examining protein folding with the aid of exogenously added synthetic peptides.
authors.affiliation: Rutgers University, Newark, NJ 07102.
authors.forename: P W
authors.initials: PW
authors.lastname: Cash
authors.forename: X
authors.initials: X
authors.lastname: Zhu
authors.forename: Y
authors.initials: Y
authors.lastname: Ohta
authors.forename: J
authors.initials: J
authors.lastname: Tsao
authors.forename: H
authors.initials: H
authors.lastname: Lackland
authors.forename: M D
authors.initials: MD
authors.lastname: Mateos-Nevado
authors.forename: M
authors.initials: M
authors.lastname: Inouye
authors.forename: S
authors.initials: S
authors.lastname: Stein
authors.forename: F
authors.initials: F
authors.lastname: Jordan
authors.forename: G I
authors.initials: GI
authors.lastname: Tous
mesh_terms: D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D003488:Cyanogen Bromide; D004792:Enzyme Precursors / Q000138:chemical synthesis* / Q000378:metabolism; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis* / Q000302:isolation & purification / Q000378:metabolism; D010449:Peptide Mapping; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D012697:Serine Endopeptidases; D013381:Subtilisins / Q000138:chemical synthesis* / Q000378:metabolism
chemical_list: D004792:Enzyme Precursors; D010446:Peptide Fragments; D010455:Peptides; C057107:prosubtilisin; D012697:Serine Endopeptidases; D013381:Subtilisins; C028344:glutamyl endopeptidase; D003488:Cyanogen Bromide