title: Distribution of omega-3 and omega-6 fatty acids in the ether- and ester-linked phosphoglycerides from tissues of the rainbow trout, Salmo gairdneri.
abstract: 1. Data presented here demonstrate that polyunsaturated fatty acids in the phospholipids of rainbow trout tissues are compartmentalized differently than in mammalian tissues. 2. We have determined the distribution of omega-3 (n-3) and omega-6 (n-6) fatty acids in the alkyl-, alk-1-enyl-, and diacyl- subclasses of phosphatidylcholines (PC), phosphatidyl-ethanolamines (PE), phosphatidylinositols (PI), and phosphatidylserines (PS) from gill, kidney and spleen of rainbow trout. 3. Alkyl-linked PC and alk-1-enyl-linked PE were the most abundant ether-containing phospholipids, amounting to 10-15% of each class; no ether-linked PI or PS was detected. 4. C20:4 n-6 was found in high concentrations only in PI; the n-3 fatty acids were found in highest concentration in the ether-linked phospholipids as compared with the diacyl subclasses and C20:5 n-3 was especially prevalent in 1-O-alk-1'-enyl-2-acyl-sn-glycero-3-phosphoethanolamine and C22:6 n-3 was prevalent in PS.
authors.affiliation: Mountain Aquaculture Research Center, Western Carolina University, Cullowhee, NC 28723.
authors.forename: M R
authors.initials: MR
authors.lastname: Turner
authors.forename: S L
authors.initials: SL
authors.lastname: Leggett
authors.forename: R H
authors.initials: RH
authors.lastname: Lumb
mesh_terms: D000818:Animals; D015525:Fatty Acids, Omega-3 / Q000378:metabolism; D043371:Fatty Acids, Omega-6; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D005880:Gills / Q000378:metabolism; D005994:Glycerophosphates / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D010955:Plasmalogens / Q000378:metabolism; D012487:Salmonidae / Q000378:metabolism*; D013154:Spleen / Q000378:metabolism; D014018:Tissue Distribution; D014337:Trout / Q000378:metabolism*
chemical_list: D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6; D005231:Fatty Acids, Unsaturated; D005994:Glycerophosphates; D010743:Phospholipids; D010955:Plasmalogens; C020791:phosphatidal ethanolamines