Molecular study on nosocomial Pseudomonas aeruginosa strains.
Nosocomial Pseudomonas aeruginosa isolates were typed by DNA fingerprinting techniques. Chromosomal DNA banding patterns, after endonuclease digestion, of six isolates from a neonatal care unit confirmed the results of O-serotyping and antimicrobial susceptibility pattern analysis. In contrast, the characterization by chromosomal DNA fingerprinting of P. aeruginosa strains isolated from patients treated in a burn unit disagreed with the two classic typing methods: in fact, isolates that varied in serotype and antibiograms showed identical restriction endonuclease profiles, whereas two indistinguishable isolates cultured from individual patients were easily and reproducibly differentiated by molecular analysis.
Nastasi
A
A
Instituto di Igiene G. D'Alessandro, Università degli Studi di Palermo, Italia.
Mammina
C
C
Villafrate
M R
MR
Scarlata
G
G
Torregrossa
M V
MV
Valentino
L
L
D015373:Bacterial Typing Techniques; D002876:Chromosomes, Bacterial; D003428:Cross Infection / Q000097:blood / Q000382:microbiology; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis; D004352:Drug Resistance, Microbial; D006801:Humans; D007231:Infant, Newborn; D011552:Pseudomonas Infections / Q000097:blood / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000302:isolation & purification*; D015183:Restriction Mapping; D013045:Species Specificity
D004269:DNA, Bacterial