title: Host cell-dependent regulation of growth transformation-associated Epstein-Barr virus antigens in somatic cell hybrids.
abstract: We have analyzed the expression of the three major known growth transformation-associated Epstein-Barr virus (EBV) proteins, EBNA-1, EBNA-2, and latent membrane protein (LMP), in a series of somatic cell hybrids derived from the fusion of EBV-carrying Burkitt lymphoma (BL) lines with EBV-positive or EBV-negative B-cell lines. Independently of the cell phenotype, EBNA-1 was invariably coexpressed in all EBV-carrying hybrids. In hybrids between EBV-carrying, LMP-positive and LMP-negative Burkitt lymphoma lines, LMP was expressed, indicating positive control. Two EBV-negative lymphoma lines, Ramos and BJAB, differed in their ability to express LMP after B95-8 virus-induced conversion and after hybridization with Raji cells. BJAB was permissive while Ramos was nonpermissive for LMP, although both expressed EBNA-2. The EBNA-2-deleted P3HR-1 virus gave the same pattern of LMP expression in these two cells. Our findings indicate that the expression of EBNA-1, EBNA-2, and LMP is regulated by independent mechanisms.
authors.affiliation: Department of Tumor Biology, Karolinska Institute, Stockholm, Sweden.
authors.forename: B
authors.initials: B
authors.lastname: Contreras-Salazar
authors.forename: G
authors.initials: G
authors.lastname: Klein
authors.forename: M G
authors.initials: MG
authors.lastname: Masucci
mesh_terms: D000956:Antigens, Viral / Q000096:biosynthesis / Q000235:genetics*; D015153:Blotting, Western; D002051:Burkitt Lymphoma; D002213:Capsid / Q000276:immunology; D002460:Cell Line; D002461:Cell Line, Transformed; D002467:Cell Nucleus / Q000276:immunology; D002472:Cell Transformation, Viral; D004591:Electrophoresis, Polyacrylamide Gel; D019309:Epstein-Barr Virus Nuclear Antigens; D005786:Gene Expression Regulation; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000276:immunology*; D006801:Humans; D006822:Hybrid Cells; D014407:Tumor Cells, Cultured; D014763:Viral Matrix Proteins
chemical_list: D000956:Antigens, Viral; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D019309:Epstein-Barr Virus Nuclear Antigens; D014763:Viral Matrix Proteins