Detection of 1q polysomy in interphase nuclei of human solid tumors with a biotinylated probe.
A biotinylated probe (L23-21) specific for the 1q12 band of human karyotype was used to detect the 1q segment in interphase nuclei of breast and colon carcinomas. This probe was selected because trisomy or polysomy 1q is the most frequent chromosomal change observed in solid tumors. This method enables cancerous cells, including near-diploid ones carrying an unbalanced rearrangement of 1q, to be easily identified.
Viegas-Péquignot
E
E
URA 620 CNRS, Structure et Mutagenèse Chromosomiques, Institut Curie, Section de Biologie, Paris, France.
Jeanpierre
M
M
Dutrillaux
A M
AM
Gerbault-Seureau
M
M
Muleris
M
M
Dutrillaux
B
B
D001710:Biotin; D001943:Breast Neoplasms / Q000235:genetics*; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000235:genetics*; D002467:Cell Nucleus / Q000648:ultrastructure; D002469:Cell Separation; D002878:Chromosomes, Human, Pair 1; D003110:Colonic Neoplasms / Q000235:genetics*; D015342:DNA Probes; D005434:Flow Cytometry; D006801:Humans; D007399:Interphase; D007621:Karyotyping; D014314:Trisomy; D014407:Tumor Cells, Cultured
D015342:DNA Probes; D001710:Biotin