[Cell cycle analysis of hybridoma cultures: study by cytofluorimetry].
An hybridoma cell line cultivated in flasks has been used as a model to study the loss of cell viability in high density cell cultures. Cell cycle analysis by cytofluorimetry has shown that a new hypochromosomic cell population appeared as soon as the viability began to decrease. However there is no evidence that this new population is constituted of dead cells.
Voisin
P
P
Unité 284, I.N.S.E.R.M., Vandoeuvre-lès-Nancy.
Eyl
V
V
Muller
S
S
Donner
M
M
Maugras
M
M
Stoltz
J F
JF
D000818:Animals; D002452:Cell Count; D002453:Cell Cycle; D003470:Culture Media; D005434:Flow Cytometry; D006825:Hybridomas / Q000166:cytology*; D051379:Mice
D003470:Culture Media
Analyse du cycle cellulaire de cultures d'hybridomes: étude par cytofluorimétrie.