Cell surface fibronectin of mouse peritoneal macrophages.
Fibronectin (FN) was detected on thioglycollate-induced mouse peritoneal macrophages by binding the 125I-labeled F(ab')2 fragment of rabbit anti-human plasma fibronectin. The cell surface fibronectin (sFN) was removed from the surface of the macrophage monolayer by limited trypsinization. After trypsinization, binding of 125I-labeled plasma fibronectin (125I-pFN) to the macrophage monolayer was increased, suggesting that the FN receptor covered with sFN was exposed by trypsinization without destroying the receptor activity. The amounts of saturation binding of 125I-pFN to the macrophage monolayers before and after trypsinization were about 2.4 and 6.3 micrograms per 10(6) cells, respectively, indicating that the macrophage monolayer has the capacity of binding 6.3 micrograms FN per 10(6) cells, and the FN receptor equivalent to about 4 micrograms pFN per 10(6) cells is covered with sFN.
Beppu
M
M
Tokyo College of Pharmacy, Japan.
Masa
H
H
Kikugawa
K
K
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D005353:Fibronectins / Q000032:analysis*; D007158:Immunologic Techniques; D008264:Macrophages / Q000032:analysis*; D051379:Mice; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic / Q000032:analysis; D013499:Surface Properties; D014357:Trypsin / Q000494:pharmacology
D005353:Fibronectins; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic; D014357:Trypsin