title: Preferential expansion of Ly-1 B and CD4- CD8- T cells in the polyclonal lymphocyte responses to murine T. cruzi infection.
abstract: Acute murine infection with T. cruzi results in polyclonal lymphocyte responses manifested by blast transformation of a large fraction of B, CD4+, and CD8+ cells. We describe here the finding of significant increases in the splenic representation of minor populations, Ly-1 + B cells and CD4-CD8- T cells. These lymphocyte populations might play an important role in the host response, as shown by T. cruzi infection of hosts that had been lethally irradiated and reconstituted with autologous bone marrow. Under these conditions, the splenic polyclonal PFC responses are nearly abrogated, and not restored by the transfer of syngeneic peritoneal cells which, however, reconstitute T15 idiotype production in the same hosts. Control levels of PFC responses, however, are reconstituted by transfer of syngeneic splenic T cells. Since bone marrow-reconstituted animals contain normal numbers of CD4+ and CD8+ T cells which are actually activated by infection, these results suggest the participation of other T cell populations in the host response to infection, as also suggested by the marked increases in T cell receptor gamma and delta messages detected in the spleen of infected animals. The implications of these findings in immunopathology of Chagas' disease are discussed.
authors.affiliation: Department of Immunology, Pasteur Institute, Paris, France.
authors.forename: P
authors.initials: P
authors.lastname: Minoprio
authors.forename: A
authors.initials: A
authors.lastname: Bandeira
authors.forename: P
authors.initials: P
authors.lastname: Pereira
authors.forename: T
authors.initials: T
authors.lastname: Mota Santos
authors.forename: A
authors.initials: A
authors.lastname: Coutinho
mesh_terms: D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte; D000950:Antigens, Ly; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D015704:CD4 Antigens; D016827:CD8 Antigens; D014355:Chagas Disease / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D011828:Radiation Chimera / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000235:genetics; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D014158:Transcription, Genetic
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D000950:Antigens, Ly; D015704:CD4 Antigens; D016827:CD8 Antigens; D011948:Receptors, Antigen, T-Cell