title: In situ hybridization and immunohistochemical study of human papillomavirus infection in adult laryngeal papillomas.
abstract: Routinely processed paraffin sections from 20 patients with adult laryngeal papillomas were examined for the presence of human papillomavirus type 11 (HPV-11) DNA and its specific mRNA by in situ hybridization methods using 35S-labeled RNA probes. Immunohistochemical techniques were also used to identify papillomavirus genus-specific common antigen (pgs-antigen). HPV-11 DNA signals and/or papillomavirus genus-specific common antigen were detected in all eight samples of multiple laryngeal papilloma. On the other hand, in 12 samples of single laryngeal papilloma, neither papillomavirus genus-specific common antigen nor HPV-11 DNA were detected. Four patients were positive for both HPV-11 DNA and pgs-antigen. In three of these four patients, HPV-11 mRNA signals were also detected. These results provided direct evidence of the association of HPV and adult multiple laryngeal papilloma.
authors.affiliation: Division of Pathology, National Cancer Center Research Institute, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Tsutsumi
authors.forename: T
authors.initials: T
authors.lastname: Nakajima
authors.forename: M
authors.initials: M
authors.lastname: Gotoh
authors.forename: Y
authors.initials: Y
authors.lastname: Shimosato
authors.forename: Y
authors.initials: Y
authors.lastname: Tsunokawa
authors.forename: M
authors.initials: M
authors.lastname: Terada
authors.forename: S
authors.initials: S
authors.lastname: Ebihara
authors.forename: I
authors.initials: I
authors.lastname: Ono
mesh_terms: D000328:Adult; D000368:Aged; D000956:Antigens, Viral / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D007822:Laryngeal Neoplasms / Q000032:analysis* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D010212:Papilloma / Q000032:analysis* / Q000276:immunology; D027383:Papillomaviridae / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D014412:Tumor Virus Infections / Q000032:analysis* / Q000276:immunology
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral