title: Identification of a promoter region for 3.6-kilobase mRNA of hepatitis B virus and specific cellular binding protein.
abstract: The promoter region for transcription of the 3.6-kilobase mRNA of hepatitis B virus was identified by the chloramphenicol acetyltransferase assay by using HuH-7 hepatoma cells and was found to function directly in virus production by way of the transient expression system of HBV. The 5'-upstream sequence from nucleotides 1573 to 1657 (the transcription start site) was indispensable for promoter function, while the AT-rich sequence (from nucleotides 1581 to 1604) containing a directly repeated sequence TGTT connecting the same flanking sequence PyAAAGAC (where Py is a pyrimidine) at both sides was an essential element within this promoter region. A specific cellular factor which interacted with the essential element was detected in the HuH-7 cell extract. A similar binding factor was also observed in HepG2 and huH2-2 hepatoma cells. This factor may thus be responsible for regulating 3.6-kilobase mRNA, pregenome RNA transcription, or both.
authors.affiliation: Department of Gene Research, Cancer Institute, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yaginuma
authors.forename: K
authors.initials: K
authors.lastname: Koike
mesh_terms: D001483:Base Sequence; D006528:Carcinoma, Hepatocellular; D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D002872:Chromosome Deletion; D004251:DNA Transposable Elements; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D008113:Liver Neoplasms; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009154:Mutation; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D016601:RNA-Binding Proteins; D014158:Transcription, Genetic
chemical_list: D002352:Carrier Proteins; D004251:DNA Transposable Elements; D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral; D016601:RNA-Binding Proteins