title: Interaction of clathrin coat proteins with unilamellar and multilamellar vesicles of phosphatidylcholine.
abstract: The binding of clathrin and accessory coat proteins to small unilamellar vesicles and to liposomes of uncharged phospholipids has been followed by chromatography, 31P-NMR, ESR and fluorescence anisotropy. At pH 6.5 and at an ionic strength value (0.1 M Mes) close to that used during the purification of clathrin-coated vesicles, the proteins do not restore the characteristic network found around the natural vesicles. Instead, a limited fusion leads to enlarged structures in which the perturbation of the dynamics of the phospholipids decreases gradually with the depth in the membrane. While the rate of motion of the outer polar heads is lowered, the order parameter of doxyl groups located either under or in the vicinity of the glycerol backbone is not affected by the proteins. In the inner core of the membrane, the main thermotropic transition of the hydrocarbon chains is unchanged. All the effects are the results of interactions limited to the membrane surface. The electrostatic nature of these interactions is evidenced when the embedded spin labels have a charge protruding at the membrane surface. An 'anchoring' effect appears which is due to the charged groups of the proteins. The lateral diffusion of the probes is reduced and, at low ionic strength, a cationic derivative no longer detects the thermotropic transition of the hydrocarbon chains. These results indicate that, although it is known that clathrin and accessory proteins bind to membranes by a series of protein-protein interactions, this system is not devoid of lipid-protein interactions, at least when it is not organized as in the natural system.
authors.affiliation: Centre de Biophysique Moléculaire, C.N.R.S., France.
authors.forename: J P
authors.initials: JP
authors.lastname: Privat
authors.forename: M
authors.initials: M
authors.lastname: Egret-Charlier
authors.forename: H
authors.initials: H
authors.lastname: Labbé
authors.forename: M
authors.initials: M
authors.lastname: Ptak
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport; D000818:Animals; D001921:Brain / Q000648:ultrastructure; D002417:Cattle; D002845:Chromatography; D002966:Clathrin / Q000378:metabolism*; D003034:Coated Pits, Cell-Membrane / Q000032:analysis; D003497:Cyclic N-Oxides; D004161:Diphenylhexatriene; D004578:Electron Spin Resonance Spectroscopy; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes; D006863:Hydrogen-Ion Concentration; D008081:Liposomes / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008854:Microscopy, Electron; D009994:Osmolar Concentration; D010713:Phosphatidylcholines / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D013113:Spin Labels; D013696:Temperature
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D002966:Clathrin; D003497:Cyclic N-Oxides; D005456:Fluorescent Dyes; D008081:Liposomes; D010713:Phosphatidylcholines; D010750:Phosphoproteins; D013113:Spin Labels; D004161:Diphenylhexatriene; C005072:5-doxylstearic acid