Rescue of a Dictyostelium discoideum mutant defective in cyclic nucleotide phosphodiesterase.
One of the developmentally induced gene products that is essential for chemotaxis of Dictyostelium amoebae is a cyclic nucleotide phosphodiesterase. The enzyme can be secreted or exist in a membrane bound form. This enzyme is missing in the mutant HPX235 which, as a consequence, does not aggregate unless exogenous cAMP phosphodiesterase is supplied. We have introduced multiple copies of the cloned phosphodiesterase gene into mutant amoebae and restored aggregation. The formation of anatomically correct fruiting bodies, which does not occur when exogenous enzyme is added, is also restored by transformation with the gene. The construct that we have used gives rise only to secreted phosphodiesterase and therefore the membrane bound form of the enzyme is not absolutely required for normal aggregation and morphogenesis.
Faure
M
M
Department of Anatomy and Cell Biology, College of Physicians and Surgeons, Columbia University, New York, New York 10032.
Podgorski
G J
GJ
Franke
J
J
Kessin
R H
RH
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000502:physiology; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D004023:Dictyostelium / Q000166:cytology / Q000201:enzymology* / Q000254:growth & development; D004229:Dithiothreitol / Q000494:pharmacology; D009154:Mutation; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics; D014170:Transformation, Genetic
D004274:DNA, Recombinant; D012333:RNA, Messenger; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D004229:Dithiothreitol