[Research in similarities between EEP34 and lipocortin].
EDTA-extractable protein (EEP) is a major extrinsic protein of lens fiber membrane. The EEP of calf lens separated three major bands by SDS-PAGE, and the molecular weights of these major bands were 34 kilodalton (kd.), 33 kd. and 32 kd. Combination of EEP with F-actin and some kinds of phospholipid containing liposomes depends on Ca2+. In two-dimensional gel electrophoresis, some spots of the calf-EEP partially overlapped with spots of lipocortin, which is a substrate for epidermal growth factor receptor/kinase and an inhibitor of phospholipase A2. The 34 kd. protein of the EEP cross-reacted to the antibody prepared against lipocortin of pig's aorta: These results suggest the important function of EEP in lens.
Takahashi
H
H
Oota
A
A
Nakayama
R
R
Kobayashi
R
R
Sakuragi
S
S
D000818:Animals; D017302:Annexins; D000906:Antibodies / Q000276:immunology; D002417:Cattle; D003429:Cross Reactions; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005136:Eye Proteins / Q000032:analysis* / Q000276:immunology; D006023:Glycoproteins / Q000032:analysis* / Q000276:immunology; D007908:Lens, Crystalline / Q000032:analysis; D008970:Molecular Weight
D017302:Annexins; D000906:Antibodies; C030327:EDTA-extractable protein (lens membrane); D005136:Eye Proteins; D006023:Glycoproteins