Patterns of healing on replanted baboon incisors coated with an allogeneic fibrin-fibronectin protein concentrate.
This study investigated the use of an allogeneic fibrin-fibronectin protein concentrate (AFFP) in preventing ankylosis and root resorption on replanted teeth during healing. In 2 adult male baboons (Papio ursinus) after extraction of the mandibular incisors, the coronal two-thirds of the exposed roots were planed to remove the remnant of the periodontal ligament and the cementum. The exposed dentinal surfaces were demineralized with citric acid at pH 1 for 3 min and notched at the junction between the planed and non-planed surfaces. Before replantation, the experimental roots and alveoli were coated with the AFFP prepared from pooled fresh-frozen baboon plasma. The animals were killed 55 days after operation and histometrical analysis was performed on serial sections cut in the buccolingual direction parallel to the long axis of the recovered specimens. An analysis of variance demonstrated significant differences between the two treatments with regard to the magnitude of ankylosis (greater for citric acid-demineralized plus AFFP-treated surfaces). There were variations in the magnitude of the different patterns of healing both between and within the experimental and control surfaces. Within the limits of the study, in this primate replantation model, the biochemical treatment of surgically exposed and demineralized root surfaces with AFFP did not enhance connective tissue attachment regeneration nor prevent dentoalveolar ankylosis and root resorption.
Ripamonti
U
U
Petit
J C
JC
D000539:Alveolar Process / Q000033:anatomy & histology; D000818:Animals; D000844:Ankylosis / Q000473:pathology; D003238:Connective Tissue / Q000033:anatomy & histology; D004848:Epithelium / Q000033:anatomy & histology; D015718:Fibrin Tissue Adhesive / Q000627:therapeutic use*; D005353:Fibronectins / Q000627:therapeutic use*; D007180:Incisor / Q000601:surgery*; D008297:Male; D010215:Papio; D010519:Periodontium / Q000033:anatomy & histology; D012038:Regeneration; D012391:Root Resorption / Q000473:pathology; D014090:Tooth Replantation / Q000379:methods*; D014092:Tooth Root / Q000033:anatomy & histology / Q000601:surgery; D014945:Wound Healing
D015718:Fibrin Tissue Adhesive; D005353:Fibronectins