Purified octamer binding transcription factors stimulate RNA polymerase III--mediated transcription of the 7SK RNA gene.
We have analyzed the upstream promoter of the human 7SK RNA gene to determine which protein factors are involved in the transcription of this gene by RNA polymerase III. Using a reconstituted in vitro system, we show directly that octamer binding transcription factors (OTFs) are required for efficient transcription and that they interact with a series of nonconsensus OTF binding sites between positions -70 and -240. The same purified factors that stimulate RNA polymerase II-dependent transcription of the histone H2b gene (OTF-1) and an immunoglobulin light chain gene (OTF-2) also stimulate 7SK transcription by RNA polymerase III. Moreover, OTF-dependent stimulation requires a sequence between positions -48 and -65 that is homologous to the proximal sequence element of the class II snRNA genes. Our findings indicate that some transcription factors are utilized in the transcription of both class II and class III genes.
Murphy
S
S
Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, New York 10021.
Pierani
A
A
Scheidereit
C
C
Melli
M
M
Roeder
R G
RG
D001483:Base Sequence; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D002872:Chromosome Deletion; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D015345:Oligonucleotide Probes; D012320:RNA Polymerase III / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000096:biosynthesis / Q000235:genetics*; D017411:Ribonucleoproteins, Small Nuclear; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic
D015345:Oligonucleotide Probes; D012261:Ribonucleoproteins; D017411:Ribonucleoproteins, Small Nuclear; D014157:Transcription Factors; D012321:DNA-Directed RNA Polymerases; D012320:RNA Polymerase III