Effect of ACTH on the stability of mRNAs encoding bovine adrenocortical P-450scc, P-45011 beta, P-45017 alpha, P-450C21 and adrenodoxin.
ACTH treatment of bovine adrenocortical cells in primary culture causes increased accumulation of mRNAs encoding cytochromes P-450SCC, P-450(11)beta, P-450(17) alpha, P-450C21 and adrenodoxin as well as increased transcriptional activity of their respective genes. In this study we have shown that ACTH does not greatly affect the half-life of mRNAs encoding P-450(11)beta, P-450(17)alpha, P-450C21 and adrenodoxin. However, in the case of P-450SCC mRNA, ACTH causes a five-fold increase in the half-life leading to a significant stabilization of P-450SCC mRNA. Thus it appears that the levels of mRNAs encoding P-450(11)beta, P-450(17)alpha, P-450C21 and adrenodoxin are regulated by ACTH primarily at the transcriptional level, while that for P-450SCC is regulated at both the transcriptional and post-transcriptional levels.
Boggaram
V
V
Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 75235.
John
M E
ME
Simpson
E R
ER
Waterman
M R
MR
D000302:Adrenal Cortex / Q000187:drug effects / Q000201:enzymology*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology*; D000325:Adrenodoxin / Q000235:genetics*; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000235:genetics; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D004355:Drug Stability; D005786:Gene Expression Regulation / Q000187:drug effects*; D006207:Half-Life; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects
D012333:RNA, Messenger; D000325:Adrenodoxin; D000324:Adrenocorticotropic Hormone; D003577:Cytochrome P-450 Enzyme System; D002786:Cholesterol Side-Chain Cleavage Enzyme