title: Effect of zearalenone on the growth of mouse embryos from blastocysts to the egg cylinder stage in vitro.
abstract: Embryos were harvested at the blastocyst stage from nontreated outbred mice and were grown in vitro for 4 days. Embryos cultured in control medium hatched and grew to the egg cylinder stage. Purified zearalenone (ZEN) added to the culture medium at concentrations of 8.5 to 68 micrograms/ml decreased the number of embryos growing, with a 50% decrease in the number growing in 32 micrograms of ZEN/ml of medium. Embryos growing in ZEN had decreased numbers of cells derived from the inner cell mass, normal growth of the trophoblast, less cellular differentiation than was seen in control embryos, and increased numbers of phagosomes. Undifferentiated cells of the inner cell mass of control and treated embryos were of the same size, as determined by morphometric analysis. Addition of 25 micrograms of estradiol/ml of culture medium caused no decrease in number of embryos growing or in embryonic size. Saturation of culture medium with ZEN (68 micrograms/ml) did not inhibit the growth of a tissue culture line of goat synovial cells. Seemingly, ZEN at concentrations near saturation inhibited the growth of mouse embryos in vitro. This effect was not duplicated with similar concentrations of estradiol and was not manifested in culture-adapted cells.
authors.affiliation: Department of Veterinary Microbiology, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907.
authors.forename: G G
authors.initials: GG
authors.lastname: Long
authors.forename: J J
authors.initials: JJ
authors.lastname: Turek
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000187:drug effects* / Q000648:ultrastructure; D004622:Embryo, Mammalian / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D005314:Embryonic and Fetal Development / Q000187:drug effects; D005260:Female; D008297:Male; D051379:Mice; D012118:Resorcinols / Q000494:pharmacology*; D015025:Zearalenone / Q000494:pharmacology*
chemical_list: D012118:Resorcinols; D015025:Zearalenone