title: Lack of phosphorylation of lipocortin I in A431 epidermoid carcinoma cells treated with phorbol esters.
abstract: To examine in vivo phosphorylation of lipocortin I we made use of a polyclonal antibody to an amino terminal peptide of lipocortin I. This antibody does not recognize any other member of the annexin protein family, and can both immunoprecipitate lipocortin I and recognize this protein on western blots. Using cleaved forms of lipocortin I, we have been able to demonstrate that protein kinase C phosphorylates this protein in vitro within the first 29 amino terminal amino acids. However, the addition of phorbol esters to A431 cells over a wide range of concentrations and for varying periods of time did not stimulate the phosphorylation of this protein. Since in vitro lipocortin I is an excellent substrate for all three isoforms, alpha, beta, gamma, of protein kinase C, the discrepancy in these findings is not secondary to the presence of varying forms of this protein kinase within different cell types.
authors.affiliation: Department of Medicine, University of Alabama, Birmingham 35294.
authors.forename: F
authors.initials: F
authors.lastname: William
authors.forename: H T
authors.initials: HT
authors.lastname: Haigler
authors.forename: A S
authors.initials: AS
authors.lastname: Kraft
mesh_terms: D017302:Annexins; D000918:Antibody Specificity; D002294:Carcinoma, Squamous Cell / Q000201:enzymology / Q000378:metabolism*; D002460:Cell Line; D003429:Cross Reactions; D006023:Glycoproteins / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007106:Immune Sera; D010766:Phosphorylation; D011233:Precipitin Tests; D011493:Protein Kinase C / Q000378:metabolism; D013379:Substrate Specificity; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
chemical_list: D017302:Annexins; D006023:Glycoproteins; D007106:Immune Sera; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate