title: Radioimmunoassay for brain natriuretic peptide (BNP) detection of BNP in canine brain.
abstract: We established a highly sensitive and specific radioimmunoassay (RIA) for BNP. Our RIA detected BNP-like immunoreactivity (-LI) in the porcine and canine brains but did not detect BNP-LI in the human, monkey or rat brain. The widespread distribution of BNP-LI was demonstrated both in the porcine and canine brains, with the highest concentration in the medulla oblongata. In contrast, the highest concentration of ANP-LI determined simultaneously was in the midbrain and the olfactory bulb. High performance-gel permeation chromatography coupled with RIA revealed that the major component of BNP-LI was eluted at the position of synthetic BNP with a small molecular weight (3K). These results indicate that the RIA for BNP serves as a useful tool to investigate physiological roles of BNP.
authors.affiliation: Department of Medicine, Kyoto University School of Medicine, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Itoh
authors.forename: K
authors.initials: K
authors.lastname: Nakao
authors.forename: Y
authors.initials: Y
authors.lastname: Saito
authors.forename: T
authors.initials: T
authors.lastname: Yamada
authors.forename: G
authors.initials: G
authors.lastname: Shirakami
authors.forename: M
authors.initials: M
authors.lastname: Mukoyama
authors.forename: H
authors.initials: H
authors.lastname: Arai
authors.forename: K
authors.initials: K
authors.lastname: Hosoda
authors.forename: S
authors.initials: S
authors.lastname: Suga
authors.forename: N
authors.initials: N
authors.lastname: Minamino
mesh_terms: D000818:Animals; D009320:Atrial Natriuretic Factor / Q000032:analysis*; D001923:Brain Chemistry; D004285:Dogs; D000882:Haplorhini; D006801:Humans; D009928:Organ Specificity; D010902:Pituitary Gland / Q000032:analysis; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D013045:Species Specificity; D013552:Swine
chemical_list: D009320:Atrial Natriuretic Factor