Transient nuclear location of the IL-2 receptor during T cell activation.
Binding of interleukin-2 (IL-2) to its membrane receptor (IL-2R) on target cells is followed by internalization of the IL-2R. The subsequent intracellular fate of IL-2R is not known. This paper describes the intracellular location of the p55 subunit of the IL-2R during IL-2 mediated T cell activation and growth of two mouse T helper clones. IL-2R was visualized by immunohistochemistry using two rat monoclonal antibodies (5A2 and 7D4). Immunostaining shows that the p55 subunit of the IL-2R is transiently present in the nucleus of activated T cells. The intranuclear location of the IL-2R suggests that the p55 subunit, either alone or in conjunction with the IL-2 or the p70 subunit, may be implicated in the regulation of gene expression involved in T cell proliferation.
Jothy
S
S
Department of Pathology, McGill University, Montreal, Quebec, Canada.
Abadie
A
A
Froussard
P
P
Duphot
M
M
Thèze
J
J
D000818:Animals; D000911:Antibodies, Monoclonal; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D003470:Culture Media; D007150:Immunohistochemistry; D007376:Interleukin-2 / Q000378:metabolism; D008213:Lymphocyte Activation; D051379:Mice; D015375:Receptors, Interleukin-2 / Q000378:metabolism*; D013601:T-Lymphocytes / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D006377:T-Lymphocytes, Helper-Inducer / Q000378:metabolism / Q000648:ultrastructure; D013997:Time Factors; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D003470:Culture Media; D007376:Interleukin-2; D015375:Receptors, Interleukin-2