The major maturation glycoprotein found on rat cauda epididymal sperm surface is linked to the membrane via phosphatidylinositol.
The experiments reported here further characterize a approximately 26[3H] kD cell surface glycoprotein that can be detected on rat cauda epididymal sperm using the galactose oxidase/NaB[3H]4 technique (1). When labeled sperm are treated with PI-PLC the 26[3H] kD is completely released from the cell. The released molecule can be recovered undegraded from incubation supernatant. Release by PI-PLC converts the hydrophobic, membrane-anchored form into a hydrophilic molecule as assessed by partition studies using Triton X114. Isoelectric focusing studies using both untreated (control) and PI-PLC treated samples shows that there is charge heterogeneity with two major peaks at pls of approximately 5.0 and approximately 4.5. We also show for the first time that the molecule persists on ejaculated cells.
Moore
A
A
Department of Cell Biology and Neuroanatomy, University of Minnesota, Minneapolis 55455.
White
T W
TW
Ensrud
K M
KM
Hamilton
D W
DW
D000818:Animals; D002474:Cell-Free System; D004542:Ejaculation; D004822:Epididymis; D006133:Growth Substances / Q000032:analysis* / Q000502:physiology; D007525:Isoelectric Focusing; D008297:Male; D016200:Maturation-Promoting Factor; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000502:physiology; D008970:Molecular Weight; D017830:Octoxynol; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010716:Phosphatidylinositols / Q000378:metabolism; D054801:Phosphoinositide Phospholipase C; D010727:Phosphoric Diester Hydrolases; D011092:Polyethylene Glycols; D051381:Rats; D011919:Rats, Inbred Strains; D013079:Sperm Maturation; D013094:Spermatozoa / Q000032:analysis* / Q000502:physiology
D006133:Growth Substances; D008562:Membrane Glycoproteins; D010716:Phosphatidylinositols; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40; D016200:Maturation-Promoting Factor; D010727:Phosphoric Diester Hydrolases; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase