Marked and variable inhibition by chemical fixation of cytochrome oxidase and succinate dehydrogenase in single motoneurons.
The effect of tissue fixation on succinate dehydrogenase and cytochrome oxidase activity in single motoneurons of the rat was demonstrated using a computer image processing system. Inhibition of enzyme activity by chemical fixation was variable, with some motoneurons being affected more than others. It was concluded that quantification of enzymatic activity in chemically fixed tissue provides an imprecise estimate of enzyme activities found in fresh-frozen tissues.
Chalmers
G R
GR
Department of Kinesiology, University of California, Los Angeles 90024.
Edgerton
V R
VR
D000818:Animals; D003576:Electron Transport Complex IV / Q000037:antagonists & inhibitors* / Q000378:metabolism; D005260:Female; D005404:Fixatives; D006651:Histocytochemistry / Q000379:methods; D009046:Motor Neurons / Q000166:cytology / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D013385:Succinate Dehydrogenase / Q000037:antagonists & inhibitors* / Q000378:metabolism
D005404:Fixatives; D013385:Succinate Dehydrogenase; D003576:Electron Transport Complex IV