title: Isolation and sequence determination of rat cardiac natriuretic peptide.
abstract: We have isolated a cardiac natriuretic peptide of 5K daltons from the rat atrium and determined its amino acid sequence. The 5K cardiac natriuretic peptide was elucidated to be a 45-amino acid peptide with the sequence of S-Q-D-S-A-F-R-I-Q-E-R-L-R-N-S-K-M-A-H-S-S-S-C-F-G-Q-K-I-D-R-I-G-A-V-S-R- L-G-C-D - G-L-R-L-F by sequencing the native peptide and its lysyl endopeptidase digests. The sequence of this peptide was identical to the amino acid sequence [51-95] of the rat brain natriuretic peptide (BNP) precursor deduced from the cDNA sequence. The 5K cardiac natriuretic peptide, or BNP[51-95], was identified as the major storage and secretory form derived from the BNP precursor in the rat heart.
authors.affiliation: Department of Medicine, Kyoto University School of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kambayashi
authors.forename: K
authors.initials: K
authors.lastname: Nakao
authors.forename: H
authors.initials: H
authors.lastname: Itoh
authors.forename: K
authors.initials: K
authors.lastname: Hosoda
authors.forename: Y
authors.initials: Y
authors.lastname: Saito
authors.forename: T
authors.initials: T
authors.lastname: Yamada
authors.forename: M
authors.initials: M
authors.lastname: Mukoyama
authors.forename: H
authors.initials: H
authors.lastname: Arai
authors.forename: G
authors.initials: G
authors.lastname: Shirakami
authors.forename: S
authors.initials: S
authors.lastname: Suga
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000302:isolation & purification* / Q000378:metabolism; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D006325:Heart Atria / Q000032:analysis*; D007158:Immunologic Techniques; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011869:Radioligand Assay; D051381:Rats
chemical_list: D009320:Atrial Natriuretic Factor