title: Enzymological characterization of a feline analogue of primary hyperoxaluria type 2: a model for the human disease.
abstract: This paper concerns an enzymological investigation into a putative feline analogue of the human autosomal recessive disease primary hyperoxaluria type 2. The hepatic activities of D-glycerate dehydrogenase, using both D-glycerate and hydroxypyruvate as substrates, and glyoxylate reductase, which are the deficient enzyme activities in human primary hyperoxaluria type 2, were markedly depleted in four affected cats (0-6% of controls). The activities of a number of other enzymes, lactate dehydrogenase, glutamate dehydrogenase, D-amino acid oxidase, aspartate:2-oxoglutarate amino-transferase, glutamate:glyoxylate aminotransferase and alanine:glyoxylate aminotransferase (the deficient enzyme in primary hyperoxaluria type 1) were unaltered. The intracellular distribution of D-glycerate dehydrogenase and glyoxylate reductase in cat liver was shown to be cytosolic, as they are in human liver. The activities of D-glycerate dehydrogenase and glyoxylate reductase were determined in unaffected related cats and putative heterozygotes were identified. The correlation between D-glycerate dehydrogenase and glyoxylate reductase activities in the related cats and their combined deficiency in the affected cats confirmed previous suggestions that they are identical gene products.
authors.affiliation: Division of Inherited Metabolic Diseases, Clinical Research Centre, Harrow, Middlesex, UK.
authors.forename: C J
authors.initials: CJ
authors.lastname: Danpure
authors.forename: P R
authors.initials: PR
authors.lastname: Jennings
authors.forename: J
authors.initials: J
authors.lastname: Mistry
authors.forename: R A
authors.initials: RA
authors.lastname: Chalmers
authors.forename: R E
authors.initials: RE
authors.lastname: McKerrell
authors.forename: W F
authors.initials: WF
authors.lastname: Blakemore
authors.forename: M F
authors.initials: MF
authors.lastname: Heath
mesh_terms: D000429:Alcohol Oxidoreductases / Q000172:deficiency*; D000818:Animals; D002237:Carbohydrate Dehydrogenases / Q000172:deficiency*; D002371:Cat Diseases / Q000201:enzymology* / Q000235:genetics; D002415:Cats; D004195:Disease Models, Animal; D006801:Humans; D006959:Hyperoxaluria / Q000201:enzymology* / Q000235:genetics; D008099:Liver / Q000201:enzymology*; D010375:Pedigree
chemical_list: D000429:Alcohol Oxidoreductases; D002237:Carbohydrate Dehydrogenases; C020830:glyoxylate reductase; C020669:Glycerate dehydrogenase