Level of expression of the tomato rbcS-3A gene is modulated by a far upstream promoter element in a developmentally regulated manner.
By Agrobacterium-mediated transformation we have demonstrated that a 1.10-kilobase promoter sequence from the tomato rbcS-3A gene confers light-inducible and organ-specific expression upon fusion to the bacterial chloramphenicol acetyltransferase gene. A biphasic expression profile was obtained by 5' deletion analysis of this promoter, indicating the presence of both positive and negative regulatory elements. A severe reduction in the level of expression was observed when the 5'-terminal 90 base pairs were deleted from the 1.10-kilobase promoter. DNA sequence elements responsible for light inducibility and organ specificity of the gene reside within the -374 base pairs of the proximal part of the promoter and the sequences spanning from -374 to -205 are essential for promoter function. The DNA sequences upstream from -374 modulate the level of expression in leaf tissue; this modulation is under developmental control.
Ueda
T
T
Department of Biology, University of Pennsylvania, Philadelphia 19104-6018.
Pichersky
E
E
Malik
V S
VS
Cashmore
A R
AR
D001483:Base Sequence; D002678:Chimera; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D003001:Cloning, Molecular; D004247:DNA; D015971:Gene Expression Regulation, Enzymologic / Q000528:radiation effects; D008027:Light; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D063245:Plant Development; D010944:Plants / Q000201:enzymology / Q000235:genetics*; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012231:Rhizobium; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics* / Q000378:metabolism; D014026:Nicotiana
D011994:Recombinant Proteins; D004247:DNA; D015500:Chloramphenicol O-Acetyltransferase; D012273:Ribulose-Bisphosphate Carboxylase