3'-Arylazido-beta-alanyl-2-azido ATP, a cross-linking photoaffinity label for F1ATPases.
The synthesis of the 3'-arylazido-2-azido ATP derivative 3'-O-(3-[N-(4-azido-2-nitrophenyl)-amino]propionyl)2-azido-adenosine 5'-triphosphate (2,3'-DiN3ATP) is described. The bifunctional photoreactive ATP analog is characterized spectroscopically. Photoaffinity labeling of F1ATPase from Micrococcus luteus by this analog results in the inactivation of the enzyme and in the formation of higher molecular weight cross-links, composed of alpha- and beta-subunits.
Schäfer
H J
HJ
Institut für Biochemie, Johannes Gutenberg-Universität, Mainz, Bundesrepublik Deutschland.
Rathgeber
G
G
Dose
K
K
Sauer
H E
HE
Trommer
W E
WE
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology; D000345:Affinity Labels / Q000138:chemical synthesis*; D001386:Azides / Q000138:chemical synthesis* / Q000378:metabolism / Q000494:pharmacology; D007202:Indicators and Reagents; D007700:Kinetics; D008027:Light; D008837:Micrococcus / Q000201:enzymology; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors / Q000378:metabolism* / Q000528:radiation effects
C026397:3'(O)-(3-(N-(4-azido-2-nitrophenyl)amino)propionyl)-8-azidoadenosine 5'-triphosphate; D000345:Affinity Labels; D001386:Azides; D007202:Indicators and Reagents; D000255:Adenosine Triphosphate; D006180:Proton-Translocating ATPases