[Research on energy metabolism enzyme in liver of the rabbit during hemorrhagic shock and effect of acupuncture at "renzhong" acupoint by quantitative histochemistry].
The energy crisis has been considered to act an important role during shock recent years. We all know the liver can supply for energy substrate when body is in an emergency condition. According to the theory of Traditional Chinese Medicine, Renzhong acupoint is a very important acupoint to treat "Jue Zheng" (including shock). We divided 36 male rabbits into three groups randomly, they were normal, acupuncture and nonacupuncture. The normal control was anesthetized only with sodium pentobarbital (35mg/kg). The acupuncture and nonacupuncture groups were operated under conscious with method of improving Wiggers(1950). After bleeding 20ml/kg, these animals were in shock. Then the acupuncture group was applied with acupuncture at "Renzhong" and the nonacupuncture group was not during hemorrhagic shock. The samples of liver were excised and stained using enzyme histochemical method. All of them were determined with MPV-2 and Univar-SMSP. Two dehydrogenases (LDH and SDH) in hepatocytes were observed in this work. The results showed that the enzyme's activity in nonacupuncture both LDH and SDH were the weakest among three groups (P less than 0.01 and P less than 0.05). But in acupuncture group, they were stronger than nonacupuncture group and near normal control. These suggest that acupuncture at "Renzhong" acupoint can increase activity of LDH and SDH during hemorrhagic shock. It is useful to resistance shock in a certain degree.
Zhai
L
L
Wen
S E
SE
Huang
W M
WM
D015669:Acupuncture Points; D015670:Acupuncture Therapy; D000818:Animals; D004734:Energy Metabolism; D006651:Histocytochemistry; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008297:Male; D011817:Rabbits; D012771:Shock, Hemorrhagic / Q000201:enzymology* / Q000628:therapy; D013385:Succinate Dehydrogenase / Q000378:metabolism
D007770:L-Lactate Dehydrogenase; D013385:Succinate Dehydrogenase