Fusion of phospholipid vesicles mediated by cytochrome c.
Fusion of phosphatidylserine/phosphatidylethanolamine (1/1) vesicles induced by cytochrome c is studied at a wide range of pH values. A pH profile for the fusion with maximum values at pH 5 and pH 8 is obtained and this is found to be similar to the profile for cytochrome c binding to the vesicles. The binding property of apocytochrome c to the same phospholipid vesicles is found to be about the same as that of the cytochrome c at low ionic strength, but very different at high salt concentrations. No appreciable fusion of vesicles by apocytochrome c is observed. Proteolytic treatment and dansyl chloride labeling of cytochrome c- and apocytochrome c-vesicle complexes show that the C-terminal segments of these proteins with molecular weights of about 3000 and 5000, respectively, penetrate the bilayer. The hydrophobic labeling studies with photoreactive phosphatidylcholine in the bilayer show that segments of both cytochrome c and apocytochrome c go deep into the bilayer.
Lee
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S
Department of Biological Science and Engineering, Korea Advanced Institute of Science and Technology, Seoul.
Kim
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H
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001059:Apoproteins / Q000032:analysis / Q000494:pharmacology; D002463:Cell Membrane Permeability; D003574:Cytochrome c Group / Q000032:analysis / Q000494:pharmacology*; D045304:Cytochromes c; D005453:Fluorescence; D008051:Lipid Bilayers / Q000032:analysis; D008561:Membrane Fusion / Q000187:drug effects*; D008563:Membrane Lipids / Q000032:analysis*; D008565:Membrane Proteins / Q000032:analysis; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D010718:Phosphatidylserines / Q000378:metabolism*; D010743:Phospholipids / Q000032:analysis*; D014466:Ultraviolet Rays
D000596:Amino Acids; D001059:Apoproteins; D003574:Cytochrome c Group; D008051:Lipid Bilayers; D008563:Membrane Lipids; D008565:Membrane Proteins; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010718:Phosphatidylserines; D010743:Phospholipids; D045304:Cytochromes c; D010447:Peptide Hydrolases