Identification of L-glutamate binding sites in chick brain by photoaffinity labeling.
The photoaffinity cross-linker, N-hydroxysuccinimidyl-4-azido benzoate ester, was used to attach L-[3H]glutamate irreversibly to chick brain membranes. Electrophoretic analysis with sodium dodecyl sulphate polyacrylamide gel electrophoresis revealed a major radioactive protein band with an apparent molecular weight of 45,600 +/- 300 Da. A second band with a smaller amount of radioactivity and with an apparent molecular weight of 28,300 +/- 500 Da was also detected. Photolabeling was inhibited by quisqualic acid.
Voukelatou
G
G
Department of Physiology, School of Medicine, University of Patras, Greece.
Aletras
A J
AJ
Kouvelas
E D
ED
D000345:Affinity Labels; D000818:Animals; D001386:Azides; D001923:Brain Chemistry; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D008970:Molecular Weight; D010777:Photochemistry; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
D000345:Affinity Labels; D001386:Azides; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter; C029534:hydroxysuccinimidyl-4-azidobenzoate