Detection of herpes simplex virus by 8 h in shell vial cultures with primary rabbit kidney cells.
Shell vial (SV) cultures for herpes simplex virus using primary rabbit kidney cells stained at 8 h after inoculation were compared with 20-h SV cultures as well as conventional tissue culture. Of the 326 clinical specimens examined, conventional culture detected 67, and of these, 61 (91%) and 42 (63%) were detected by 20-h SV cultures and 8-h SV cultures, respectively.
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Department of Pathology, University of California Irvine Medical Center, Orange 92668-3297.
Aarnaes
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de la Maza
L M
LM
Peterson
E M
EM
D000818:Animals; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D006801:Humans; D011237:Predictive Value of Tests; D011817:Rabbits; D018139:Simplexvirus / Q000254:growth & development / Q000302:isolation & purification*; D013997:Time Factors