[Boron neutron capture therapy using 10B entrapped anti-CEA immunoliposome].
A new murine monoclonal antibody (2C-8) was prepared by immunizing mice ip with CEA producing human pancreatic cancer cell line, AsPC-1.SDS-PAGE and Western blot analysis showed that 2C-8 monoclonal antibody recognized CEA and NCA. This anti-CEA monoclonal antibody was conjugated with large multilamellar liposomes incorporated 10B compound (Cs2 10B12H11SH). This immunoliposomes applicated to boron neutron capture therapy. AsPC-1 cells were incubated with the 10B-Lip-MoAb(CEA) for 8 hours. After the irradiation with thermal neutron (1 x 10(11)-1 x 10(13) n/cm2), boronated AsPC-1 cells were showed decreasing uptake of 3H-TdR compared with control group. The numbers of 10B atoms in liposomes bound to an antibody were in proportion to the dose of 10B compounds added and maximum number of 10B atoms was approximatory 1.2 x 10(4)/Ab. These data indicated that the immunoliposomes could deliver highly amount of 10B atoms to the tumor cells and exert cytotoxic effect by thermal neutron. BNCT with immunoliposome may be useful to the non resectable malignant tumors in clinical application.
Yanagie
H
H
Department of Clinical Oncology, University of Tokyo, Japan.
Fujii
Y
Y
Takahashi
T
T
Tomita
T
T
Fukano
Y
Y
Hasumi
K
K
Nariuchi
H
H
Yasuda
T
T
Sekiguchi
M
M
Uchida
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000627:therapeutic use; D001895:Boron / Q000008:administration & dosage* / Q000627:therapeutic use; D002272:Carcinoembryonic Antigen / Q000276:immunology*; D004337:Drug Carriers; D006801:Humans; D007554:Isotopes; D008081:Liposomes; D051379:Mice; D009502:Neutrons; D010190:Pancreatic Neoplasms / Q000628:therapy*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen; D004337:Drug Carriers; D007554:Isotopes; D008081:Liposomes; D001895:Boron