title: Localization of type III procollagen mRNA in areas of liver fibrosis by in situ hybridization.
abstract: Localization of type III procollagen mRNA in human liver was studied by in situ hybridization using human alpha 1(III) procollagen cDNA. Frozen and paraformaldehyde-fixed sections of biopsied human liver from patients with chronic hepatitis and cirrhosis were examined using the digoxigenin-labeled cDNA probe. Localization of the type III procollagen mRNA was demonstrated not only in the cytoplasm of mesenchymal cells but also in a large number of hepatocytes, in proportion to the extent of fibrosis. These results suggest that hepatocytes play an important role in fibrogenesis in the liver.
authors.affiliation: Department of Pathology, Nippon Medical School, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yamada
authors.forename: T
authors.initials: T
authors.lastname: Aida
authors.forename: K
authors.initials: K
authors.lastname: Taguchi
authors.forename: G
authors.initials: G
authors.lastname: Asano
mesh_terms: D015342:DNA Probes; D003856:Deoxyuracil Nucleotides; D004076:Digoxigenin; D006526:Hepatitis C / Q000378:metabolism / Q000473:pathology*; D006525:Hepatitis, Viral, Human / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000032:analysis / Q000166:cytology / Q000473:pathology*; D008103:Liver Cirrhosis / Q000378:metabolism / Q000473:pathology*; D009693:Nucleic Acid Hybridization; D011347:Procollagen / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D015342:DNA Probes; D003856:Deoxyuracil Nucleotides; D011347:Procollagen; D012333:RNA, Messenger; D004076:Digoxigenin