title: The murine retinoblastoma homolog maps to chromosome 14 near Es-10.
abstract: Restriction fragment length variants have been exploited to map genetically Rb-1, the murine homolog of the human retinoblastoma gene. Rb-1 localized to mouse chromosome 14 on the basis of results from analysis of somatic cell hybrids. In an interspecific backcross involving Mus spretus, Rb-1 and the murine homolog of the human esterase D gene (ESD), which we refer to here as Esd, were inseparable. Furthermore, the strain distribution patterns of Rb-1 and Es-10 are the same in 31 of 32 recombinant inbred strains. Close linkage of the chromosome 14 morphological marker hairless (hr) to Rb-1 is also implied. These results localize Rb-1 on the mouse linkage map and provide close genetic markers to follow Rb-1 in somatic as well as in germline genetic experiments. Additionally, the results suggest that Es-10 is the murine homolog of ESD and provide further evidence for linkage conservation during mammalian evolution.
authors.affiliation: Jackson Laboratory, Bar Harbor, Maine 04609.
authors.forename: J C
authors.initials: JC
authors.lastname: Stone
authors.forename: J L
authors.initials: JL
authors.lastname: Crosby
authors.forename: C A
authors.initials: CA
authors.lastname: Kozak
authors.forename: A R
authors.initials: AR
authors.lastname: Schievella
authors.forename: R
authors.initials: R
authors.lastname: Bernards
authors.forename: J H
authors.initials: JH
authors.lastname: Nadeau
mesh_terms: D000818:Animals; D005075:Biological Evolution; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000235:genetics; D002874:Chromosome Mapping; D006224:Cricetinae; D003433:Crosses, Genetic; D015342:DNA Probes; D004195:Disease Models, Animal; D005260:Female; D008040:Genetic Linkage; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009115:Muridae / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D012175:Retinoblastoma / Q000235:genetics*
chemical_list: D015342:DNA Probes; D005819:Genetic Markers; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; C020118:ESD protein, human