Glycine potentiates NMDA responses in rat hippocampal CA1 neurons.
When superfused onto rat hippocampal slices, glycine (0.1-0.5 mM) potentiated the depolarization induced by pressure application of NMDA in normal Krebs solution and the synaptic discharge evoked by stimulation of the Schaffer collateral-commissural inputs to the CA1 pyramidal neurons bathed in Mg2+-free media; the effects were not prevented by strychnine. In addition, glycine partially reversed the blocking effect of D-2-amino-5-phosphonovalerate (AP5) on N-methyl-D-aspartate (NMDA)-induced depolarization. These results show that glycine at relatively high concentrations potentiates the NMDA-mediated response in hippocampal slices.
Minota
S
S
Department of Pharmacology, Loyola University Stritch School of Medicine, Maywood, IL 60153.
Miyazaki
T
T
Wang
M Y
MY
Read
H L
HL
Dun
N J
NJ
D015763:2-Amino-5-phosphonovalerate; D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D005998:Glycine / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D016202:N-Methylaspartate; D051381:Rats; D014633:Valine / Q000031:analogs & derivatives / Q000494:pharmacology
D001224:Aspartic Acid; D016202:N-Methylaspartate; D015763:2-Amino-5-phosphonovalerate; D014633:Valine; D005998:Glycine