Somatostatin inhibition of VIP-induced somatostatin release, cyclic AMP accumulation and 45Ca2+ uptake in diencephalic cells.
The effect of somatostatin (SRIF) on VIP induction of SRIF secretion, cyclic AMP accumulation and 45Ca2+ influx was investigated in cultured diencephalic cells. [D-Trp8]SRIF suppressed VIP-stimulated SRIF release and decreased VIP-stimulated cyclic AMP accumulation in a dose-dependent manner. SRIF-14 blocked basal and VIP-stimulated 45Ca2+ entry into cells. The data suggest that the inhibitory effect of SRIF on VIP-induced SRIF release is partly due to a decrease in Ca2+ entry into cells.
Pares-Herbute
N
N
U.A. 1197 CNRS, University of Montpellier II, France.
Diaz
J
J
Astier
H
H
Tapia-Arancibia
L
L
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002132:Calcium Radioisotopes; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism*; D004027:Diencephalon / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005333:Fetus / Q000378:metabolism; D011247:Pregnancy; D051381:Rats; D013004:Somatostatin / Q000378:metabolism / Q000494:pharmacology*; D013997:Time Factors; D014660:Vasoactive Intestinal Peptide / Q000037:antagonists & inhibitors* / Q000494:pharmacology
D002132:Calcium Radioisotopes; D014660:Vasoactive Intestinal Peptide; D013004:Somatostatin; D000242:Cyclic AMP; D002118:Calcium