Expression of c-erbA in human hepatocellular carcinomas.
The expression of the oncogene c-erbA was studied in six hepatocellular carcinoma specimens and normal hepatic tissue. Total RNA isolated from these samples was analysed by Northern as well as slot blot hybridisation to a radioactive c-erbA probe. When compared to normal tissue, expression in the tumour and tissue adjacent to tumour was markedly elevated. These results suggest that overexpression of c-erbA is related to hepatocarcinogenesis. Southern blot analysis of DNA from the tumours gave no evidence of c-erbA rearrangements.
Arbuthnot
P
P
Department of Medical Biochemistry, University of the Witwatersrand Medical School, Parktown, South Africa.
Kew
M
M
Parker
I
I
Fitschen
W
W
D015152:Blotting, Northern; D015139:Blotting, Southern; D006528:Carcinoma, Hepatocellular / Q000032:analysis / Q000235:genetics*; D006801:Humans; D008099:Liver / Q000032:analysis*; D008113:Liver Neoplasms / Q000032:analysis / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000235:genetics*; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000032:analysis; D011988:Receptors, Thyroid Hormone; D012016:Reference Values; D014158:Transcription, Genetic
D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D011988:Receptors, Thyroid Hormone