DNA duplex with the potential to change handedness after every half a turn.
Polymorphic forms of the DNA duplex with long stretches of structural monotony are known. Several alternating purine-pyrimidine sequences have been shown to adopt left-handed Z-conformation. We report a DNA sequence d(CGCGCGATCGAT)n exhibiting alternating right-handed B and left-handed Z helical conformation after every half a turn. Further, this unusual conformation with change in handedness after every six base pairs was induced at physiological superhelical density.
Brahmachari
S K
SK
Molecular Biophysics Unit, Indian Institute of Science, Bangalore.
Mishra
R K
RK
Bagga
R
R
Ramesh
N
N
D001483:Base Sequence; D004247:DNA / Q000648:ultrastructure*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004278:DNA, Superhelical / Q000648:ultrastructure; D004587:Electrophoresis, Agar Gel; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010957:Plasmids; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism
D004278:DNA, Superhelical; D004247:DNA; D004262:DNA Restriction Enzymes; D015719:Single-Strand Specific DNA and RNA Endonucleases