The effect of thyroid hormones on the membrane adenylate cyclase activity in sickle red blood cells in vitro.
A comparative study of thyroid hormone responsive membrane adenylate cyclase activity has been carried out on normal (Hb-AA) and sickle cell (Hb-SS) subjects. Thyroid hormones (T3 and T4) and selected analogues (TRIAC and DLT) enhanced adenylate cyclase activity in Hb-AA membranes. The interaction of Hb-SS membranes with the hormones and analogues was not significant except for T3 which moderately stimulated Hb-SS membrane adenylate cyclase. We suggest that circulating irreversibly sickled cells, in view of their membrane phospholipid defect, probably contribute to the low response of membrane adenylate cyclase to effector stimulation.
Oladapo Olawoye
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Department of Chemical Pathology, College of Medicine, University of Ibadan, Nigeria.
Dada
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OA
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000755:Anemia, Sickle Cell / Q000097:blood*; D000242:Cyclic AMP / Q000096:biosynthesis; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000201:enzymology*; D006801:Humans; D066298:In Vitro Techniques; D013974:Thyroxine / Q000494:pharmacology*; D014284:Triiodothyronine / Q000031:analogs & derivatives / Q000494:pharmacology*; D014443:Tyrosine / Q000494:pharmacology
D014284:Triiodothyronine; C010642:3,3',5-triiodothyroacetic acid; D014443:Tyrosine; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D013974:Thyroxine