title: Effect of porcine brain natriuretic peptide (pBNP) on human adrenocortical steroidogenesis.
abstract: Porcine brain natriuretic peptide (pBNP), purified from porcine brain, had a significant suppressive effect on aldosterone and cortisol secretions in ACTH-treated cultured human adrenal cells. Concomitantly, the intracellular cGMP formation was enhanced by pBNP treatment. A specific pBNP receptor was identified in the human adrenal tissues. Affinity labelling of 125I-pBNP showed two separate molecular weights of specific binding sites for pBNP of 140 and 67 kDa. A 125I-pBNP binding study of the human adrenal membrane fraction demonstrated the presence of high-affinity and low-capacity binding sites for pBNP. Moreover, these binding sites for 125I-pBNP were displaced by unlabelled alpha-rANP as well as pBNP. From these studies, we concluded that pBNP had suppressive effects on human adrenocortical steroidogensis, possibly via a receptor which may be shared with ANP.
authors.affiliation: Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Hashiguchi
authors.forename: K
authors.initials: K
authors.lastname: Higuchi
authors.forename: M
authors.initials: M
authors.lastname: Ohashi
authors.forename: R
authors.initials: R
authors.lastname: Takayanagi
authors.forename: H
authors.initials: H
authors.lastname: Matsuo
authors.forename: H
authors.initials: H
authors.lastname: Nawata
mesh_terms: D000302:Adrenal Cortex / Q000187:drug effects*; D000305:Adrenal Cortex Hormones / Q000096:biosynthesis*; D000345:Affinity Labels; D000450:Aldosterone / Q000378:metabolism; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D006801:Humans; D006854:Hydrocortisone / Q000378:metabolism; D066298:In Vitro Techniques; D011956:Receptors, Cell Surface / Q000378:metabolism*
chemical_list: D000305:Adrenal Cortex Hormones; D000345:Affinity Labels; D011956:Receptors, Cell Surface; D000450:Aldosterone; D009320:Atrial Natriuretic Factor; D006854:Hydrocortisone