Variation in herpes simplex virus type 1 glycoproteins produced in kidney cells from different species.
Viral glycoproteins from herpes simplex virus, type 1 (HSV-1) infected NBL-1, Vero, and BHK-21 cells were labelled with 14C-glucosamine and studied by SDS-PAGE and Con-A chromatography. SDS-PAGE analysis demonstrated differences in the number and molecular weight of glycoproteins from these cells. Con-A chromatography resulted in similar binding of glycoproteins from NBL-1 and Vero cells of 10.5 and 18.6%, respectively, whereas BHK-21 cells showed binding of 65%. These studies indicate that HSV-1 glycoprotein oligosaccharide processing is variable in kidney cells of different species.
Al-Ahdal
M N
MN
Department of Biological and Medical Research, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudia Arabia.
Al-Khodairy
F A
FA
McGarry
T J
TJ
D000818:Animals; D002460:Cell Line; D002846:Chromatography, Affinity; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000096:biosynthesis*; D007668:Kidney / Q000166:cytology / Q000382:microbiology*; D018139:Simplexvirus / Q000378:metabolism*; D014709:Vero Cells; D014764:Viral Proteins / Q000096:biosynthesis*
D006023:Glycoproteins; D014764:Viral Proteins