Amplification of oncogenes in lung carcinoma grafted in nude mice.
Seven lung carcinomas were grafted on nude mice and continuously propagated as in vivo models on which the amplification of 9 oncogenes (N-myc, v-erb A, v-abl, v-sis, c-myc, c-myb, v-Ha-ras, c-Kiras, and v-scr) was studied by Southern blot hybridization. Only c-myc was amplified (20 copies) in an adenocarcinoma. The presence of 2 bands at 9 kb and 6.6 kb in addition to the normal 12.7 kb in EcoR1 digested DNAs suggested a polymorphism of the c-myc gene in this tumor. The other 8 oncogenes were not amplified in this tumor. The 5 small cell lung carcinomas of this study did not show any amplification of any of the 9 oncogenes tested.
Chauvin
C
C
Département de Biophysique, UFR de Médecine de Grenoble, La Tronche, France.
Jacrot
M
M
Riondel
J
J
Brambilla
E
E
Foote
A M
AM
Brambilla
C
C
Benabid
A L
AL
D000230:Adenocarcinoma / Q000235:genetics*; D000818:Animals; D018288:Carcinoma, Small Cell / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D005784:Gene Amplification; D008175:Lung Neoplasms / Q000235:genetics*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009857:Oncogenes; D012150:Polymorphism, Restriction Fragment Length; D011518:Proto-Oncogene Proteins / Q000235:genetics; D016271:Proto-Oncogene Proteins c-myc
D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc