Cytidylate cyclase activity in mouse tissues: the enzymatic conversion of cytidine 5'-triphosphate to cytidine 3',5'-cyclic monophosphate (cyclic CMP).
Cytidylate cyclase activity, which enzymatically converts cytidine 5'-triphosphate (CTP) to cytidine 3',5'-cyclic monophosphate (cyclic CMP), has been demonstrated in mouse tissue homogenates by use of a highly sensitive enzyme immunoassay (EIA) specific for cyclic CMP. Cyclic CMP formation is dependent on the amount of homogenate and on the incubation time. Although the enzyme activity was detected at wide ranges of pH from 6.8 to 11.5, the maximal activity was observed at around pH 9.4. The optimal temperature was 37 degrees C. Cytidylate cyclase activity was almost completely lost if the homogenates were heated at 90 degrees C for 3 min prior to use. The enzyme reaction exhibited typical Michaelis-Menten kinetics with an apparent Km for CTP of approx. 0.31 mM. Cyclic CMP formation was greatly enhanced with 4 mM Mn2+, Mg2+, Co2+; Mn2+ was the most effective. Fe2+ and Ca2+ were without effect. Cu2+ and Zn2+ at a concentration of 0.1 to 0.5 mM were inhibitory to Mn2+-dependent activity. Moreover, the enzyme activity was inhibited by several nucleotides including ATP, ADP, 5'-AMP, and GTP. Cytidylate cyclase activity was found to be present in all homogenates from a variety of mouse tissues examined except heart, with the highest level found in brain, and the lowest in liver.
Yamamoto
I
I
Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Takai
T
T
Mori
S
S
D000818:Animals; D001921:Brain / Q000201:enzymology; D002413:Cations, Divalent; D003035:Cobalt / Q000494:pharmacology; D003563:Cyclic CMP / Q000378:metabolism*; D003570:Cytidine Triphosphate / Q000378:metabolism*; D003597:Cytosine Nucleotides / Q000378:metabolism*; D005260:Female; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007124:Immunoenzyme Techniques; D007700:Kinetics; D008190:Lyases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D019761:Phosphorus-Oxygen Lyases; D013379:Substrate Specificity; D013696:Temperature; D014018:Tissue Distribution
D002413:Cations, Divalent; D003597:Cytosine Nucleotides; D003563:Cyclic CMP; D003035:Cobalt; D008345:Manganese; D003570:Cytidine Triphosphate; D008190:Lyases; D019761:Phosphorus-Oxygen Lyases; C019978:cytidylate cyclase; D008274:Magnesium