title: Construction and characterization of versatile kanamycin-resistance cassettes derived from the Tn5 transposon.
abstract: We have developed plasmids with the Tn5 kanamycin-resistance gene (kan) flanked either symmetrically or asymmetrically by several restriction sites. These can be used to provide a selectable genetic marker or to mobilize restriction sites and sense or nonsense codons into genes. The 1.3-kb kan cassette exhibits polarity effects in both directions.
authors.affiliation: Department of Biological Sciences, University of South Carolina, Columbia 29208.
authors.forename: R E
authors.initials: RE
authors.lastname: Musso
authors.forename: T
authors.initials: T
authors.lastname: Hodam
mesh_terms: D001483:Base Sequence; D003062:Codon; D004251:DNA Transposable Elements; D007613:Kanamycin Resistance / Q000235:genetics*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D015183:Restriction Mapping
chemical_list: D003062:Codon; D004251:DNA Transposable Elements; D015345:Oligonucleotide Probes