[Mutants of Escherichia coli K-12 with altered excision efficiency of transposons Tn5 and Tn9].
HFETn5, HFETn9 and LFETn9 mutants of Escherichia coli K-12 have been isolated. The frequency of Tn5 precise excision from the chromosomal lac operon is increased 3-660-fold in nine HFETn5 mutants. The majority of these mutations have no influence on the efficiency of precise excision of transposon Tn9, though hfeTn5-04 and hfeTn5-06 mutations decrease excision efficiency 2-13-fold. The Tn9 transposon is excised in HFETn9 mutant about 20-fold more efficiently than in the wild type strain. This mutation does not stimulate excision of Tn5 and Tn10. LfeTn9 mutation decreases excision frequency of Tn9 11-17-fold, but has no effect on Tn5 excision and increases that of Tn10 about 20-fold. The differences in genetic control and mechanisms of excision of the transposons with long and short inverted repeats are discussed.
Mukhamedshin
E K
EK
Tarasov
V A
VA
D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D009154:Mutation; D009693:Nucleic Acid Hybridization; D009876:Operon; D010957:Plasmids
D004251:DNA Transposable Elements
Mutanty Escherichia coli K-12 s izmenennoĭ éffektivnost'iu tochnogo vyrezaniia transpozonov Tn5 i Tn9.