Beta-adrenergic stimulation of phagocytosis in the unicellular eukaryote Paramecium aurelia.
Bete-adrenergic agonists isoproterenol and norepinephrine enhanced phagocytosis in Paramecium. Stimulation was stereospecific, dose-dependent and inhibited by the beta-agonists propranolol and alprenolol. Phorbol ester and forskolin potentiated the stimulatory effect of catecholamines on Paramecium phagocytosis. The dansyl analogue of propranolol (DAPN) was used for fluorescent visualization of the beta-adrenergic receptor sites in Paramecium which have been found to be localized at the cell membrane and within the membrane of the nascent digestive vacuoles. The appearance of the characteristic fluorescent pattern has been blocked by 1-propranolol.
Wyroba
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Department of Cell Biology, Nencki Institute of Experimental Biology, Warsaw, Poland.
D000526:Alprenolol / Q000494:pharmacology; D000818:Animals; D001665:Binding Sites; D003994:Bucladesine / Q000494:pharmacology; D005576:Colforsin / Q000494:pharmacology; D003619:Dansyl Compounds / Q000378:metabolism; D007545:Isoproterenol / Q000494:pharmacology*; D009638:Norepinephrine / Q000494:pharmacology*; D010247:Paramecium / Q000187:drug effects* / Q000502:physiology; D010587:Phagocytosis / Q000187:drug effects*; D010703:Phorbol Esters / Q000494:pharmacology; D011433:Propranolol / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D011943:Receptors, Adrenergic, beta / Q000032:analysis
D003619:Dansyl Compounds; D010703:Phorbol Esters; D011943:Receptors, Adrenergic, beta; D005576:Colforsin; D003994:Bucladesine; C061695:1-((2-(5-dimethylamino)naphthalene-1-sulfonylaminoethyl)amino)-3-(1-naphthaleneoxy)-2-propanol; D000526:Alprenolol; D011433:Propranolol; D007545:Isoproterenol; D009638:Norepinephrine