title: Molecular cloning and expression of a locus (mdoA) implicated in the biosynthesis of membrane-derived oligosaccharides in Escherichia coli.
abstract: Mutants of Escherichia coli defective in the mdoA locus are blocked at an early stage in the biosynthesis of membrane-derived oligosaccharides. The mdoA locus has now been cloned into multicopy plasmids. A 5 kb DNA fragment is necessary to complement mdoA mutations. Cells harbouring the mdoA+ plasmid produced three to four times more MDO than wild-type cells. MDO overproduction did not affect the degree of MDO substitution with sn-1-phosphoglycerol residues. The biosynthesis of MDO remained under osmotic control in overproducing strains.
authors.affiliation: Institut de Microbiologie, URA 136 CNRS, Université Paris-Sud, Orsay, France.
authors.forename: J M
authors.initials: JM
authors.lastname: Lacroix
authors.forename: M
authors.initials: M
authors.lastname: Tempête
authors.forename: B
authors.initials: B
authors.lastname: Menichi
authors.forename: J P
authors.initials: JP
authors.lastname: Bohin
mesh_terms: D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D008566:Membranes / Q000378:metabolism; D009154:Mutation; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000235:genetics; D009997:Osmotic Pressure; D010957:Plasmids; D015183:Restriction Mapping; D014161:Transduction, Genetic
chemical_list: D004251:DNA Transposable Elements; D009844:Oligosaccharides