[A modification of the ABC method (avidin-biotin-peroxidase complex) for the detection of viral antigens in coronavirus infections in cats (FIP) and parvovirus type 2 infections in dogs].
A modified ABC-method (avidin-biotin-peroxidase complex) is established to demonstrate viral antigens in paraffin sections of tissues of coronavirus (FIP) infected cats and parvovirus type 2 infected dogs using monoclonal antibodies. The pros and cons and potential sources of error are described and discussed.
Walter
J
J
Dohse
K
K
Rudolph
R
R
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002371:Cat Diseases / Q000175:diagnosis*; D002415:Cats; D003332:Coronaviridae / Q000276:immunology; D003333:Coronaviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D007124:Immunoenzyme Techniques; D010321:Parvoviridae / Q000276:immunology; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000662:veterinary*
D000956:Antigens, Viral
Eine Modifikation der ABC-Methode (Avidin-Biotin-Peroxidase-Complex) für den Nachweis von viralen Antigenen bei der Infektion der Katze durch ein Coronavirus (FIP) und der Infektion des Hundes durch das Parvovirus-Typ 2.