Distribution of glycosyltransferase activities in different compartments of mitochondria.
The liver mitochondria were submitted to a first swelling which allowed to get outer membranes. The mitoplasts obtained in these conditions were subject to a second swelling. The separation of submitochondrial membranes on a discontinuous sucrose gradient revealed three membrane fractions, an outer membrane rich fraction, an inner membrane rich fraction and a fraction enriched with contact sites between the two membranes. The various glycosyltransferase systems involved in the biosynthesis of N-glycoproteins were located in these fractions.
Levrat
C
C
Department of Biochemistry, University of Lyon, INSERM-CNRS U 189. Lyon-Sud Medical School, Oullins, France.
Louisot
P
P
Morelis
R
R
D000818:Animals; D001665:Binding Sites; D002451:Cell Compartmentation; D002499:Centrifugation, Density Gradient; D006023:Glycoproteins / Q000096:biosynthesis; D006593:Hexokinase / Q000378:metabolism; D006602:Hexosyltransferases / Q000378:metabolism*; D008358:Mannose / Q000378:metabolism; D008364:Mannosyltransferases / Q000378:metabolism; D051379:Mice; D008854:Microscopy, Electron; D008930:Mitochondria, Liver / Q000201:enzymology* / Q000648:ultrastructure; D008933:Mitochondrial Swelling; D010770:Phosphotransferases / Q000378:metabolism; D013347:Subcellular Fractions / Q000201:enzymology; D017855:Transferases (Other Substituted Phosphate Groups)
D006023:Glycoproteins; D006602:Hexosyltransferases; D008364:Mannosyltransferases; D010770:Phosphotransferases; D006593:Hexokinase; D017855:Transferases (Other Substituted Phosphate Groups); C023222:UDPacetylglucosamine-dolichyl-phosphate acetylglucosamine-1-phosphate transferase; D008358:Mannose