Genetic relatedness among Mycobacterium tuberculosis and M. bovis.
Total DNA from two slowly-growing pathogenic mycobacterial species propagated in vitro was isolated, digested with each of 34 restriction endonucleases and analyzed by agarose gel electrophoresis. The most resolved patterns for M. tuberculosis (ATCC 27294) and for M. bovis (ATCC 19210) were obtained respectively using (BamHI, DraI, ClaI, EcoRI, EcoRV, HindIII, HpaI, SalI, SmaI, XbaI and XmaI). The patterns produced for these strains were reproducible and distinguishable from each other respectively using (HindIII, DraI, EcoRI, MboI, Sau3AI and AvaI). However, with several enzymes (SalI, AsuI, Sau96I, MspI and HpaII) the patterns for M. tuberculosis and M. bovis were similar. Evidence was obtained for the presence of dam and dcmI methylations in the DNA of each mycobacterial species respectively using (MboI, Sau3AI, EcoRII, BstNI, Sau96I and AsuI).
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University of Texas Health Center, Department of Microbiology, Tyler 75710.
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D005075:Biological Evolution; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D008745:Methylation; D009163:Mycobacterium bovis / Q000235:genetics*; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D013045:Species Specificity
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes