[Cloning and sequence analysis of cDNA of common precursors of three hypothalamic neuropeptides immunologically related to human somatocrinin 1-37, alpha melanotropin and salmon melanin-concentrating hormone].
We have cloned and sequenced DNAs complementary to the mRNA encoding the precursor of the rat melanin-concentrating hormone. This allowed us to elucidate the primary structure of the 96 C-terminal residues of this precursor. It contains three possible sites for enzymatic cleavage enabling the generation of MCH and of two additional neuropeptides. These three peptides can bind one of our antisera raised against human somatocrinin 1-37, alpha-melanotropin and salmon melanin-concentrating hormone, which immunocytochemically stain the same neuron population in the hypothalamus.
Breton
C
C
C.N.R.S., U.R.A. n. 0561, Faculté de Médecine et Pharmacie, Besançon.
Fellmann
D
D
Bugnon
C
C
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D013007:Growth Hormone-Releasing Hormone / Q000276:immunology; D006801:Humans; D007028:Hypothalamic Hormones; D008543:Melanins / Q000276:immunology; D008969:Molecular Sequence Data; D010443:Peptide Chain Termination, Translational; D010907:Pituitary Hormones / Q000276:immunology; D011355:Prodrugs; D051381:Rats; D012474:Salmon / Q000378:metabolism; D000521:alpha-MSH / Q000276:immunology
D007028:Hypothalamic Hormones; D008543:Melanins; D010907:Pituitary Hormones; D011355:Prodrugs; D000521:alpha-MSH; C019463:melanin-concentrating hormone; D004247:DNA; D013007:Growth Hormone-Releasing Hormone
Clonage et séquençage d'ADNc du précurseur commun de trois neuropeptides hypothalamiques immunologiquement apparentés à la somatocrinine humaine 1-37, à l'alpha-mélanotropine et à l'hormone de mélanoconcentration du saumon.