Alpha 1-adrenergic receptor binding sites in post-mortal human cerebral microvessel preparations: preservation in multi-infarct dementia and dementia of Alzheimer type.
Cerebral cortical microvessels were prepared from control, dementia of Alzheimer type (AD/SDAT) and multi-infarct dementia (MID) autopsy cases. The microvessel yields were approx. 200 micrograms protein/g starting material, and did not differ significantly between control, MID and AD/SDAT groups. The purity of the preparations was confirmed both by light and electron microscopy and by measurement of enrichment of the endothelial markers gamma-glutamyltranspeptidase and alkaline phosphatase. Higher microvessel alkaline phosphatase activities and higher microvessel/homogenate ratios of activities of both enzymes in the MID and the AD/SDAT samples than in the control samples were found, which may be consistent with previous findings of structural abnormalities of the cerebral endothelial cells in AD/SDAT. The levels of [3H]prazosin binding did not differ significantly between control. MID and AD/SDAT samples at any [ligand] tested (0.05, 0.1, and 0.5 nM), suggesting conservation of microvessel alpha 1-adrenoceptors in MID and AD/SDAT.
O'Neill
C
C
Department of Geriatric Medicine, Karolinska Institutet, Huddinge Sjukhus, Sweden.
Fowler
C J
CJ
Winblad
B
B
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000378:metabolism* / Q000503:physiopathology; D002540:Cerebral Cortex / Q000098:blood supply* / Q000378:metabolism / Q000503:physiopathology; D002560:Cerebrovascular Circulation; D003000:Clonidine / Q000031:analogs & derivatives / Q000378:metabolism; D003704:Dementia / Q000378:metabolism* / Q000503:physiopathology; D006801:Humans; D008833:Microcirculation; D011180:Postmortem Changes; D011224:Prazosin / Q000378:metabolism; D011942:Receptors, Adrenergic, alpha / Q000378:metabolism*
D011942:Receptors, Adrenergic, alpha; C016986:apraclonidine; D003000:Clonidine; D011224:Prazosin