99mTc-labelling of leucocytes with 99mTc-DPO: a complex developed for myocardial imaging.
The lipophilic 99mTc-DPO complex, developed as a myocardial imaging radiopharmaceutical, was used to label leucocytes. After an incubation of 0.1 ml 99mTc-DPO (8 micrograms DMPE*2HCl) with mixed leucocytes in plasma, the labelling efficiency was over 70%. During incubation in 5 ml plasma, a loss of activity was found between 20% (1 h) and 35% (3 h) caused by elution. Disturbances of cell viability could not be found with the help of the chemiluminescence test. The in vivo recovery was determined in three dogs and was 45%-50% (0.5 h), 30%-36% (1 h), and 18%-24% (3 h). Autologous 99mTc-DPO-leucocytes were used on seven patients with suspected osteomyelitis, there were four true negative and three true positive results. The target/nontarget ratio determined by ROI in the positive cases was 1.8 to 2.5 at 3 h after injection.
Endert
G
G
Department of Nuclear Medicine, Medical Academy Erfurt, German Democratic Republic.
Franke
U
U
Kleinert
P
P
D001842:Bone and Bones / Q000000981:diagnostic imaging; D006321:Heart / Q000000981:diagnostic imaging*; D006801:Humans; D007962:Leukocytes; D008099:Liver / Q000000981:diagnostic imaging; D008353:Mannitol; D009942:Organometallic Compounds; D015609:Organotechnetium Compounds; D010019:Osteomyelitis / Q000000981:diagnostic imaging; D010070:Oxalates; D010720:Phosphines; D011237:Predictive Value of Tests; D011877:Radionuclide Imaging; D013670:Sodium Pertechnetate Tc 99m; D013154:Spleen / Q000000981:diagnostic imaging; D013667:Technetium
D009942:Organometallic Compounds; D015609:Organotechnetium Compounds; D010070:Oxalates; D010720:Phosphines; D008353:Mannitol; D013667:Technetium; C031898:technetium Tc 99m bis(1,2-dimethylphosphino)ethane; D013670:Sodium Pertechnetate Tc 99m