Large-scale mapping and chromosome jumping in the q27 region of the human X chromosome.
We have used pulsed field gel electrophoresis for further physical mapping studies in the q27 region of the human X chromosome. We show that the DXS 102 locus and the F9 gene are separated by only 300 kb despite a genetic distance of 1.4 cM; this linkage orients our large-scale map and shows that the mcf.2 transforming sequence is telomeric to F9. A BssHII complete-digest jumping library was used to jump toward the DXS 105 locus; a 130-kb jump was achieved and the corresponding "linking clone" was obtained.
Nguyen
C
C
Centre d'Immunologie de Marseille Luminy INSERM-CNRS, France.
Poustka
A M
AM
Djabali
M
M
Roux
D
D
Mattei
J F
JF
Lehrach
H
H
Jordan
B R
BR
D015139:Blotting, Southern; D002874:Chromosome Mapping; D015342:DNA Probes; D004586:Electrophoresis; D008040:Genetic Linkage; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length; D014960:X Chromosome / Q000648:ultrastructure*
D015342:DNA Probes