Biological function of the rotavirus protein VP4: observations on porcine isolates from China.
Rotaviruses isolated from pigs in China were grown in MA104 cells. One tissue-culture-adapted isolate consisted of two subpopulations (variants), the RNA profiles of which differed in the relative migration of RNA segment 4 only. The variants were separated by plaque purification and by recovery from limiting dilutions and remained genetically stable. The variant possessing the slower migrating RNA segment 4, called 4S, grew faster and formed large plaques after 4-6 days incubation, whereas the variant possessing the faster migrating RNA segment 4, called 4F, grew more slowly and formed only microscopic plaques after 10-14 days incubation. The protein product of the 4F RNA occurred in much lower concentration in infected cells than the product of the 4S RNA. The RNA segments 4 of the two variants were found to be closely related when tested by dot hybridization under stringent conditions. The 4S RNA is more resistant to denaturation with methyl mercuric hydroxide than is the 4F RNA. The relevance of these findings to the biological functions of rotaviruses is discussed.
Haddow
J
J
Institute of Virology, University of Glasgow, UK.
Clark
B
B
Ni
Y
Y
Desselberger
U
U
D000818:Animals; D002213:Capsid / Q000502:physiology*; D036022:Capsid Proteins; D002681:China; D015342:DNA Probes; D004591:Electrophoresis, Polyacrylamide Gel; D005838:Genotype; D006801:Humans; D008767:Methylmercury Compounds; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D012401:Rotavirus / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology*; D012692:Serial Passage; D013045:Species Specificity; D013552:Swine / Q000382:microbiology; D010948:Viral Plaque Assay
D036022:Capsid Proteins; D015342:DNA Probes; D008767:Methylmercury Compounds; D012367:RNA, Viral; C060339:VP4 protein, Rotavirus; C020771:methylmercury hydroxide