Double antigen localization of two catecholamine enzymes and GABA in amacrine cells of the rat retina in semi-thin sections.
A double immunohistochemical labeling technique was developed with two immunoperoxidase reactions performed before and after embedding in epoxy resin using two different chromogens: diaminobenzidine (yellowish brown color) and diaminobenzidine + nickel ammonium sulfate (black color). The two catecholamine enzyme immunoreactivities (phenylethanolamine-N-methyltransferase, and tyrosine-hydroxylase) were not found in the same cells, while gamma-aminobutyric acid (GABA) immunoreactivity was observed in large tyrosine-hydroxylase immunoreactive cells, but not in phenylethanolamine-N-methyltransferase immunoreactive cells.
Simon
A
A
INSERM U 86-Laboratoire de Neurocytologie Oculaire, Paris, France.
Versaux-Botteri
C
C
Denoroy
L
L
Vigny
A
A
Nguyen-Legros
J
J
D015100:3,3'-Diaminobenzidine; D000818:Animals; D002395:Catecholamines / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods*; D010625:Phenylethanolamine N-Methyltransferase / Q000378:metabolism*; D051381:Rats; D012160:Retina / Q000166:cytology / Q000378:metabolism*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D002395:Catecholamines; D015100:3,3'-Diaminobenzidine; D005680:gamma-Aminobutyric Acid; D014446:Tyrosine 3-Monooxygenase; D010625:Phenylethanolamine N-Methyltransferase