Lung cell reactions in guinea pigs after inhalation of asbestos (amosite).
To study the long-term effect of inhaled asbestos, guinea-pigs were exposed to airborne amosite at a concentration of 49 mg/m3, 2 h/day for 3 or 6 weeks and examined up to 2 years after exposure. Macrophages in lung lavage fluid (LLF) were increased at 16, 24 and 93 weeks and lymphocytes at 24 weeks. Examination of lung wall cells (LWC) 2 years after exposure compared to cells obtained by LLF showed higher proportions of LWC lymphocytes and neutrophils. Percoll gradient centrifugation of these cells showed a higher proportion of high density macrophages in LLF from the amosite-exposed animal and an increased number of low density lymphocytes in the LW. Cathepsin D was increased in LLF at 8 and 24 weeks and in alveolar macrophages 24 weeks and 2 years after exposure. Fibroblast cultures exposed to LLF did not show any statistical significant changes in their collagen synthesis. Histology 93 weeks after exposure showed macrophage and mediastinal lymph node accumulation of asbestos, as well as collagen in alveolar walls. Granulomas were found in the vicinity of blood vessels and in connection with the bronchioles. The data suggest that amosite at low doses ultimately causes fibrosis with a reaction pattern different from that seen in silicosis. The inflammation and fibrosis seems to develop only within the interstitium.
Sjöstrand
M
M
Department of Environmental Hygiene, University of Gothenburg, Sweden.
Rylander
R
R
Bergström
R
R
D000280:Administration, Inhalation; D000818:Animals; D001194:Asbestos / Q000633:toxicity*; D017639:Asbestos, Amosite; D005347:Fibroblasts / Q000187:drug effects; D006168:Guinea Pigs; D008168:Lung / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D008247:Lysosomes / Q000201:enzymology; D008264:Macrophages / Q000187:drug effects; D011392:Proline / Q000378:metabolism; D011658:Pulmonary Fibrosis / Q000209:etiology
D017639:Asbestos, Amosite; D001194:Asbestos; D011392:Proline