Identification and regulation of the eukaryotic hyaluronate synthase.
Hyaluronate synthesis is required for fibroblast detachment in mitosis and migration. It is regulated by the activity of the synthase which is localized at the inner side of plasma membranes. The synthase was identified as a 50 kDa protein by immunological cross-reaction with the streptococcal enzyme and by affinity labelling. Transformation of fibroblasts by Rous sarcoma virus activated the synthase by enhanced transcription and phosphorylation. The synthase was a natural target of pp60v-src kinase.
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Institut für Physiologische Chemie, Münster, Federal Republic of Germany.
D000345:Affinity Labels; D000818:Animals; D002471:Cell Transformation, Neoplastic; D002642:Chick Embryo; D004789:Enzyme Activation; D005347:Fibroblasts / Q000201:enzymology; D005455:Fluorescent Antibody Technique; D014453:Glucuronosyltransferase / Q000302:isolation & purification / Q000378:metabolism*; D016695:Glycosyltransferases; D000076002:Hyaluronan Synthases; D015151:Immunoblotting; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism*; D010766:Phosphorylation; D001357:Sarcoma, Avian / Q000201:enzymology; D014166:Transferases; D029867:Xenopus Proteins
D000345:Affinity Labels; D008565:Membrane Proteins; D029867:Xenopus Proteins; D014166:Transferases; D016695:Glycosyltransferases; D014453:Glucuronosyltransferase; C056944:HAS1 protein, Xenopus; D000076002:Hyaluronan Synthases