Synergism between protein kinase C activator and fatty acids in stimulating superoxide anion production in guinea pig polymorphonuclear leukocytes.
Treatment of guinea pig polymorphonuclear leukocytes (PMNL) with various fatty acids elicited superoxide anion (O2-) production and an increase in intracellular Ca2+ [( Ca2+]i). Both responses, however, were seldom observed when PMNL were treated at lower concentrations. But, simultaneous addition of 1-oleoyl-2-acetylglycerol (OAG), a protein kinase C activator, caused an increase in O2- production even at the lower concentrations of fatty acids. In contrast to the synergism in O2- production, [Ca2+]i remained at almost the basal level irrespective of the presence of OAG. Among saturated fatty acids, those with carbon numbers of 14 to 18 were most effective in stimulating O2- production in combination with OAG. Unsaturated fatty acids with a carbon number of 18 were almost equally effective irrespective of the number of double bonds.
Ozawa
M
M
Department of Physiological Chemistry, Hiroshima University School of Medicine, Japan.
Ohtsuka
T
T
Okamura
N
N
Ishibashi
S
S
D000818:Animals; D000838:Anions; D002118:Calcium / Q000378:metabolism; D004075:Diglycerides / Q000494:pharmacology*; D004357:Drug Synergism; D004789:Enzyme Activation; D005227:Fatty Acids / Q000494:pharmacology*; D005260:Female; D005989:Glycerides / Q000494:pharmacology*; D006168:Guinea Pigs; D009504:Neutrophils / Q000378:metabolism*; D010529:Peritoneal Cavity / Q000166:cytology; D013481:Superoxides / Q000378:metabolism*
D000838:Anions; D004075:Diglycerides; D005227:Fatty Acids; D005989:Glycerides; D013481:Superoxides; C038237:1-oleoyl-2-acetylglycerol; D002118:Calcium