Approaches to characterize by density-fractionation age-dependent properties of reticulocyte mitochondria.
Mitochondria from rabbit reticulocytes of varying maturity were prepared by means of two different methods and separated in sucrose and Percoll density gradients. A mechanical method of cell disintegration under isotonic conditions followed by centrifugation in a Percoll density gradient proved to be adequate to reflect the physiological conditions. "Young" mitochondria exhibit in this system greater density and higher specific activity of cytochrome oxidase.
Belkner
J
J
Institute of Biochemistry, Humboldt University Berlin, GDR.
Rapoport
S M
SM
D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002499:Centrifugation, Density Gradient / Q000379:methods; D003576:Electron Transport Complex IV / Q000097:blood; D004905:Erythrocyte Aging; D008928:Mitochondria / Q000201:enzymology / Q000648:ultrastructure*; D011205:Povidone; D011817:Rabbits; D012156:Reticulocytes / Q000201:enzymology / Q000502:physiology / Q000648:ultrastructure*; D012822:Silicon Dioxide; D013395:Sucrose
D013395:Sucrose; C016039:Percoll; D012822:Silicon Dioxide; D003576:Electron Transport Complex IV; D011205:Povidone