Analysis of expression of hybrid yeast genes containing ARS elements.
In an attempt to devise a new assay for ARS-binding proteins we have inserted the HO ARS between the upstream activation site and the TATA region of the yeast CYC1 promoter. A marked reduction in promoter activity is observed. Inactivation of the HO ARS element by point mutation does not restore promoter activity to its original level, although a modest activation is seen. We have also inserted the HO ARS into the intron of the yeast actin gene; although there is no apparent deleterious effect on transcription, the activity of the ARS is abolished in this new environment.
Kipling
D
D
Department of Zoology, Oxford, UK.
Kearsey
S E
SE
D015152:Blotting, Northern; D002352:Carrier Proteins / Q000378:metabolism*; D004261:DNA Replication; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000032:analysis
D002352:Carrier Proteins; D004274:DNA, Recombinant; D001616:beta-Galactosidase