Flagellar movement in demembranated preparations of ejaculated fowl spermatozoa.
Triton X-100 at a concentration of 0.1% in the extraction medium was optimal for demembranating fowl spermatozoa. The most suitable conditions for reactivation were obtained when a medium composed of 0.5 mM-ATP, 25 mM-potassium glutamate, 10(-7) M-CaCl2, 20 mM-Tris-HCl(pH 7.9), 1 mM-MgSO4, 1 mM-dithiothreitol and 0.2 M-sucrose was used. More than 60% motile spermatozoa were obtained under these conditions. The addition of 1 or 10 microM-cAMP did not appreciably affect motility. Intact and demembranated spermatozoa were immotile at 40 degrees C, whilst at 30 degrees C motility was restored.
Ashizawa
K
K
Laboratory of Animal Reproduction, Faculty of Agriculture, Miyazaki University, Japan.
Suzuki
Y
Y
Okauchi
K
K
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002462:Cell Membrane / Q000502:physiology; D002478:Cells, Cultured; D002645:Chickens / Q000502:physiology*; D000242:Cyclic AMP / Q000494:pharmacology; D008297:Male; D017830:Octoxynol; D011092:Polyethylene Glycols; D012503:Saponins; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000502:physiology* / Q000648:ultrastructure; D013696:Temperature
D012503:Saponins; D011092:Polyethylene Glycols; D017830:Octoxynol; D000242:Cyclic AMP; D002118:Calcium