Nutritional and hormonal regulation of mRNA abundance for arginine biosynthetic enzymes in kidney.
Argininosuccinate synthetase and argininosuccinate lyase catalyze the synthesis of arginine from citrulline in kidney and also serve as components of the urea cycle in liver of ureotelic animals. Dietary and hormonal regulation of mRNAs encoding these enzymes have been well studied in liver but not in kidney. Messenger RNAs for these enzymes are localized within the renal cortex. Starvation and extreme variations in dietary protein content (0% vs 60% casein) produced 2.6- to 3.5-fold increases in mRNA abundance for these two enzymes in rat kidney. Argininosuccinate lyase mRNA was not induced by dibutyryl cAMP, dexamethasone, or a combination of the two agents. In contrast, argininosuccinate synthetase mRNA was induced 2-fold by dibutyryl cAMP but was unresponsive to dexamethasone. Thus, diet and hormones regulate levels of these mRNAs in rat kidney, but the responses are both qualitatively and quantitatively distinct from the responses previously reported for rat liver.
Morris
S M
SM
Department of Microbiology, University of Pittsburg, Pennsylvania 15261.
Moncman
C L
CL
Holub
J S
JS
Hod
Y
Y
D000818:Animals; D001123:Argininosuccinate Lyase / Q000235:genetics* / Q000378:metabolism; D001124:Argininosuccinate Synthase / Q000235:genetics* / Q000378:metabolism; D015152:Blotting, Northern; D015139:Blotting, Southern; D003994:Bucladesine / Q000494:pharmacology; D002645:Chickens; D003001:Cloning, Molecular; D015342:DNA Probes; D003907:Dexamethasone / Q000494:pharmacology; D004044:Dietary Proteins / Q000008:administration & dosage; D004790:Enzyme Induction; D005508:Food Deprivation; D007668:Kidney / Q000187:drug effects / Q000201:enzymology*; D007672:Kidney Cortex / Q000201:enzymology; D008025:Ligases / Q000235:genetics*; D008099:Liver / Q000201:enzymology; D008190:Lyases / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D015183:Restriction Mapping
D015342:DNA Probes; D004044:Dietary Proteins; D012333:RNA, Messenger; D003994:Bucladesine; D003907:Dexamethasone; D008190:Lyases; D001123:Argininosuccinate Lyase; D008025:Ligases; D001124:Argininosuccinate Synthase