Lymphocyte beta-adrenoceptor/effector complex in aging and dementia of the Alzheimer type.
We examined density (Bmax), affinity (Kd), and norepinephrine (NE)-stimulated cyclic adenosine monophosphate (cAMP) generation of the beta-adrenergic receptor on lymphocytes in patients with dementia of the Alzheimer type (DAT, n = 13), in normal aged controls (AGED, n = 27), and in young controls (YOUNG, n = 21). Bmax (fmol/mg protein; mean +/- SD) was significantly lower in YOUNG (48.0 +/- 18.7) than in either AGED (64.8 +/- 22.3) or DAT (63.6 +/- 19.5, p less than 0.05). Receptor sensitivity, i.e., the fold increase of stimulated over basal cAMP generation (S/B), was higher in DAT (8.3 +/- 5.2) than in AGED (5.5 +/- 2.3) or YOUNG (5.4 +/- 2.6, p less than 0.07). This difference was due to a significant increase in S/B in female but not male DAT patients compared with AGED (p less than 0.05). There were no differences in levels of circulating NE between DAT and AGED, nor between sexes. Thus, the present data suggest that in this model of the postsynaptic beta-adrenergic receptor/effector complex, receptor sensitivity was increased in women with DAT.
Gietzen
D W
DW
Department of Psychiatry, University of California, Davis 95616.
Fregeau
D
D
Goodman
T
T
Weiler
P G
PG
Graf
K
K
Magliozzi
J
J
D000328:Adult; D000368:Aged; D000375:Aging / Q000378:metabolism*; D000544:Alzheimer Disease / Q000378:metabolism*; D000242:Cyclic AMP / Q000096:biosynthesis; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology; D008214:Lymphocytes / Q000378:metabolism*; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D012016:Reference Values; D012727:Sex Characteristics
D011943:Receptors, Adrenergic, beta; D000242:Cyclic AMP; D007545:Isoproterenol