Effect of cisplatin, lipopolysaccharide, muramyl dipeptide and recombinant interferon-gamma on murine macrophages in vitro. II. Production of H2O2, O2- and interleukin-1.
Murine macrophage monolayers treated with cisplatin, lipopolysaccharide (LPS), muramyl dipeptide (MDP) or recombinant interferon-gamma (rIFN gamma) for 2-48 h showed significant increases in the release of H2O2, O2- and interleukin-1 (IL-1) as compared to untreated macrophages. The treatment of macrophages with different combinations of the above agents did not induce synergistic or additive effects on the production of H2O2, O2- and IL-1. The priming of macrophages with rIFN gamma had a significant effect in the additional increased production of H2O2, O2- and IL-1 when subsequently treated with cisplatin, LPS or MDP.
Sodhi
A
A
Department of Zoology, Banaras Hindu University, Varanasi, India.
Geetha
B
B
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000494:pharmacology; D000818:Animals; D002945:Cisplatin / Q000494:pharmacology*; D004357:Drug Synergism; D006861:Hydrogen Peroxide / Q000096:biosynthesis*; D007371:Interferon-gamma / Q000494:pharmacology*; D007375:Interleukin-1 / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D008809:Mice, Inbred C3H; D011994:Recombinant Proteins; D013481:Superoxides / Q000378:metabolism*; D013997:Time Factors
D007375:Interleukin-1; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D013481:Superoxides; D000119:Acetylmuramyl-Alanyl-Isoglutamine; D007371:Interferon-gamma; D006861:Hydrogen Peroxide; D002945:Cisplatin