Loss of heterozygosity at polymorphic chromosomal loci in patients with malignant melanoma.
One type of aberration of DNA found in human cancers is loss of heterozygosity at chromosomal loci. This loss suggests that inactivation of particular genes is involved in the genesis of cancer cells. We have analyzed tumor DNA and normal DNA from eight patients with malignant melanomas using 24 polymorphic DNA probes and detected loss of heterozygosity in three patients at five loci on four different chromosomes. These results indicate that the frequency of loss of heterozygosity at chromosomal loci is relatively high in malignant melanomas, but that the regions lost are not restricted to specific chromosomes.
Orita
M
M
Oncogene Division, National Cancer Center Research Institute, Tokyo, Japan.
Murakami
Y
Y
Ishihara
K
K
Sekiya
T
T
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002869:Chromosome Aberrations; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D015342:DNA Probes; D005260:Female; D006579:Heterozygote; D006801:Humans; D008297:Male; D008545:Melanoma / Q000235:genetics*; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012878:Skin Neoplasms / Q000235:genetics*
D015342:DNA Probes