[Primary culture of hepatocytes of newborn rats as a system for modeling anoxia and metabolic ischemia].
A primary culture of hepatocytes from new born rats was used as an experimental model suitable for studies of the cell lysosomal apparatus and of molecular mechanisms involving cyclic nucleotides for initiation of lysosomal response to extremal conditions. Substrate deficiency and anoxia of the cultivated hepatocytes were found to cause distinct alterations in the state of lysosomal apparatus accompanied by an increase in free activity of acid phosphatase.
Goncharenko
T M
TM
Nikulina
S E
SE
Krylova
O Iu
OIu
Stvolinskaia
N S
NS
Poliakova
E D
ED
Korovkin
B F
BF
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D015687:Cell Hypoxia; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D005456:Fluorescent Dyes; D007511:Ischemia / Q000378:metabolism*; D008099:Liver / Q000098:blood supply / Q000166:cytology / Q000378:metabolism*; D008247:Lysosomes / Q000201:enzymology / Q000378:metabolism; D008954:Models, Biological; D051381:Rats
D005456:Fluorescent Dyes; D000242:Cyclic AMP; D000135:Acid Phosphatase
Pervichnaia kul'tura gepatotsitov novorozhdennykh krys kak sistema dlia modelirovaniia anoksii i metabolicheskoĭ ishemii.