Modulation of adenylate cyclase activity in bovine lens epithelial cells.
Adenylate cyclase activity of bovine lens epithelial cells is activated by guanine nucleotides or fluoride. Additional activation to that of guanylylimidodiphosphate [Gpp(NH)p] was induced by isoproterenol. Insulin was found to inhibit adenylate cyclase activity. Adenosine at low concentrations (5-50 nM) increased adenylate cyclase activity, while at high concentrations it lowered the activity of the enzyme. The occurrence of R and P adenosine sites in lens epithelial cells is suggested. Like other tissues, lens epithelial cells possess a transduction system (adenylate cyclase-G protein complex) for external messages.
Bizec
J C
JC
Centre de Neurochimie du CNRS, Strasbourg, France.
Klethi
J
J
Mandel
P
P
D000241:Adenosine / Q000494:pharmacology; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D000242:Cyclic AMP / Q000032:analysis; D006152:Cyclic GMP / Q000032:analysis; D004837:Epinephrine / Q000494:pharmacology; D004847:Epithelial Cells; D005459:Fluorides / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D007545:Isoproterenol; D007908:Lens, Crystalline / Q000201:enzymology*; D009924:Organ Culture Techniques; D010727:Phosphoric Diester Hydrolases / Q000032:analysis
D007328:Insulin; D000242:Cyclic AMP; D010727:Phosphoric Diester Hydrolases; D000262:Adenylyl Cyclases; D006152:Cyclic GMP; D000241:Adenosine; D007545:Isoproterenol; D005459:Fluorides; D004837:Epinephrine