Neutrophil Mac-1 and MEL-14 adhesion proteins inversely regulated by chemotactic factors.
The neutrophil Mac-1 and gp100MEL-14 adhesion proteins are involved in neutrophil extravasation during inflammation. Both the expression and activity of Mac-1 are greatly increased after neutrophil activation. In contrast, neutrophils shed gp100MEL-14 from the cell surface within 4 minutes after activation with chemotactic factors or phorbol esters, releasing a 96-kilodalton fragment of the antigen into the supernatant. Immunohistology showed that gp100MEL-14 was downregulated on neutrophils that had extravasated into inflamed tissue. The gp100MEL-14 adhesion protein may participate in the binding of unactivated neutrophils to the endothelium; rapid shedding of gp100MEL-14 may prevent extravasation into and damage of normal tissues by activated neutrophils.
Kishimoto
T K
TK
Department of Pathology, Stanford University, CA 94305.
Jutila
M A
MA
Berg
E L
EL
Butcher
E C
EC
D000818:Animals; D000943:Antigens, Differentiation / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D001854:Bone Marrow Cells; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules; D002630:Chemotactic Factors / Q000502:physiology*; D003182:Complement C5 / Q000502:physiology; D015936:Complement C5a; D005455:Fluorescent Antibody Technique; D007375:Interleukin-1 / Q000502:physiology; D016209:Interleukin-8; D007700:Kinetics; D007975:Leukotriene B4 / Q000502:physiology; D008070:Lipopolysaccharides / Q000502:physiology; D008213:Lymphocyte Activation; D008262:Macrophage Activation; D016177:Macrophage-1 Antigen; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000166:cytology / Q000276:immunology*; D013755:Tetradecanoylphorbol Acetate; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology
D000943:Antigens, Differentiation; D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D002630:Chemotactic Factors; D003182:Complement C5; D007375:Interleukin-1; D016209:Interleukin-8; D008070:Lipopolysaccharides; D016177:Macrophage-1 Antigen; D014409:Tumor Necrosis Factor-alpha; D007975:Leukotriene B4; D015936:Complement C5a; D013755:Tetradecanoylphorbol Acetate