title: DNA topoisomerases and models of sister-chromatid exchange.
abstract: Pommier et al. (1985) suggested that sister-chromatid exchange (SCE) results from exchange of topoisomerase II subunits. "Homologous displacement", an alternative mechanism, is proposed in which strand switching occurs during removal of parental helical turns by topoisomerases. The steps in the SCE model proposed by Ishii and Bender (1980) for SCE occurring at a blocked replication fork could occur by this mechanism and would require the action of both topoisomerases I and II. Homologous displacement involving topoisomerase II alone provides a mechanism for the strand switching required in the models of Kato (1977) and Cleaver (1981) in which SCE occur between replicated double strands. These mechanisms and models are discussed in relation to current knowledge of the locations and functions of topoisomerases during DNA replication.
authors.affiliation: Radiobiology Laboratory, Johns Hopkins Oncology Center, Baltimore, MD 21205.
authors.forename: L E
authors.initials: LE
authors.lastname: Dillehay
authors.forename: D
authors.initials: D
authors.lastname: Jacobson-Kram
authors.forename: J R
authors.initials: JR
authors.lastname: Williams
mesh_terms: D004261:DNA Replication; D004264:DNA Topoisomerases, Type I / Q000378:metabolism*; D004250:DNA Topoisomerases, Type II / Q000378:metabolism*; D008957:Models, Genetic; D012093:Replicon; D012854:Sister Chromatid Exchange
chemical_list: D004264:DNA Topoisomerases, Type I; D004250:DNA Topoisomerases, Type II