Antagonism of the cytotoxic but not antiviral effects of ara-sangivamycin by adenosine.
Inhibition of DNA synthesis by ara-sangivamycin was antagonized by adenosine. The 50% inhibitory concentrations increased 1.6- to 32-fold in the presence of 1.0 to 50 microM adenosine, respectively. In contrast, the inhibition of human cytomegalovirus replication by ara-sangivamycin was not antagonized by as much as 50 microM adenosine. This suggests that different enzymes were responsible for the phosphorylation of ara-sangivamycin in uninfected and infected cells.
Birch
G M
GM
Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor 48109-1078.
Krawczyk
S H
SH
Townsend
L B
LB
Drach
J C
JC
D000241:Adenosine / Q000494:pharmacology*; D000248:Adenosine Kinase / Q000037:antagonists & inhibitors; D000998:Antiviral Agents / Q000037:antagonists & inhibitors*; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D007624:KB Cells / Q000187:drug effects; D007700:Kinetics; D011741:Pyrimidine Nucleosides / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D010948:Viral Plaque Assay
D000998:Antiviral Agents; D011741:Pyrimidine Nucleosides; D004247:DNA; C061595:ara-sangivamycin; D000248:Adenosine Kinase; D000241:Adenosine; D013936:Thymidine