DNA polymorphism analysis of hereditary multiple exostoses in horses.
Genomic DNA polymorphisms obtained by restriction fragment-length polymorphism from healthy horses and horses with hereditary multiple exostoses were analyzed. These DNA were digested by 12 restriction enzymes and were hybridized against 6 isotopically labeled oncogene probes. Hybridization was not detected with the viral oncogene, v-ras, which indicated this oncogene was absent in the equine genome. Oncogenes (c-raf-1, c-fes, c-myb, c-myc, and c-sis) were present and had similar hybridization patterns and signal intensities in DNA from healthy horses and horses with hereditary multiple exostoses. Unique and distinct restriction fragment-length polymorphisms were detected with the c-raf-1 probe only in BamHI- and PstI-digested equine DNA.
Li
J K
JK
Molecular Biology/Biochemistry Program, Utah State University, Logan 84322-5500.
Moloney
B K
BK
Shupe
J L
JL
Gardner
E J
EJ
Leone
N C
NC
Elsner
Y
Y
D000818:Animals; D004247:DNA / Q000235:genetics*; D005097:Exostoses, Multiple Hereditary / Q000235:genetics / Q000662:veterinary*; D005260:Female; D006734:Horse Diseases / Q000235:genetics*; D006736:Horses / Q000235:genetics; D008297:Male; D009693:Nucleic Acid Hybridization; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
D004247:DNA