[Participation of the uvrD gene in the precise excision of the Tn9 transposon].
HfeTn5-(04,06) and IfeTn9 mutations increase efficiency of precise excision of Tn5, Tn10 and decrease that of Tn9. These mutations have been mapped in uvrD gene. In LFETn9 and UVRE502 mutants, the multicopy plasmid pEM61 carrying the cloned uvrD gene complements LFETn9- phenotype (Low Frequency Excision of Tn9). These results indicate that the uvrD gene product plays different role in excision of transposons with long and short inverted repeats. The mechanism of this effect is discussed.
Mukhamedshin
E K
EK
Tarasov
V A
VA
D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D009154:Mutation; D010957:Plasmids; D015183:Restriction Mapping
D004251:DNA Transposable Elements
Uchastie gena uvrD v tochnom vyrezanii transpozona Tn9.