Gangliosides attenuate NMDA receptor-mediated excitatory amino acid release in cultured cerebellar neurons.
Release of both D-[3H]aspartate and endogenous amino acids was measured in primary cultures of cerebellar granule cells. Two hour-pretreatment with the glycosphingolipids, GM1 or GT1b, attenuated the stimulation of excitatory amino acid release induced by depolarizing concentrations of K+ (50 mM). Gangliosides inhibited the phencyclidine (PCP)-sensitive component of depolarization-induced release, i.e. the amplification of release that follows activation of NMDA receptors by the endogenous glutamate.
Nicoletti
F
F
Institute of Pharmacology, University of Catania, School of Medicine, Italy.
Cavallaro
S
S
Bruno
V
V
Virgili
M
M
Catania
M V
MV
Contestabile
A
A
Canonico
P L
PL
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005732:Gangliosides / Q000494:pharmacology*; D005971:Glutamates / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D010622:Phencyclidine / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate; D017981:Receptors, Neurotransmitter / Q000502:physiology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000596:Amino Acids; D005732:Gangliosides; D005971:Glutamates; D016194:Receptors, N-Methyl-D-Aspartate; D017981:Receptors, Neurotransmitter; D001224:Aspartic Acid; D011189:Potassium Chloride; D010622:Phencyclidine; D013755:Tetradecanoylphorbol Acetate