Quantitation of the host cell infiltration kinetics of the nonimmunogenic colon 26 tumor by multiparameter flow cytometry.
In this study, we examined the kinetics of host cell infiltration into the nonimmunogenic Colon 26 tumor. We found that 1 x 10(4) cells were required to produce tumors in 100% of mice. The vivo doubling time was 42.5 h, and a barely palpable tumor contained 8 x 10(6) cells. No evidence of concomitant immunity was found. The number of host cells infiltrating the in vivo tumors increased at the same rate as the number of tumor cells, but averaged only 22% of total cells. Cycling T lymphocytes were present in the host cell infiltrate of this tumor. In addition, approximately 50% of in vivo Colon 26 cells were Thy-1.2 positive. The observed characteristic of low immunogenicity makes it a useful murine model for studying human malignant tumors.
Sneed
R A
RA
Life Sciences Division, Los Alamos National Laboratory, New Mexico.
Stevenson
A P
AP
Stewart
C C
CC
D000818:Animals; D000943:Antigens, Differentiation; D015214:Antigens, Differentiation, Myelomonocytic / Q000276:immunology; D000951:Antigens, Neoplasm / Q000032:analysis; D000954:Antigens, Surface; D003110:Colonic Neoplasms / Q000276:immunology* / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis; D005434:Flow Cytometry / Q000379:methods; D007156:Immunologic Memory; D016177:Macrophage-1 Antigen; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D016028:Receptors, Leukocyte-Adhesion / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology*; D018800:Thy-1 Antigens; D014407:Tumor Cells, Cultured
D000943:Antigens, Differentiation; D015214:Antigens, Differentiation, Myelomonocytic; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D004273:DNA, Neoplasm; D016177:Macrophage-1 Antigen; D016028:Receptors, Leukocyte-Adhesion; D018800:Thy-1 Antigens