Cytopathic effects induced by Epstein-Barr virus replication in epithelial nasopharyngeal carcinoma hybrid cells.
NPC-KT cl.S61, a subclone derived from an epithelial-nasopharyngeal carcinoma hybrid cell line (NPC-KT), showed cytopathic changes characteristic of herpesvirus replication, including formation of multinucleated giant cells and inclusion bodies, when Epstein-Barr virus replicative cycle was induced by 5-iodo-2'-deoxyuridine. Acyclovir (an inhibitor of herpesvirus DNA polymerase), Epstein-Barr virus-immune human serum, or 2-deoxyglucose (an inhibitor of the glycosylation) interfered with syncytium formation, indicating that a virus-specified glycoprotein belonging to the late group is responsible for cell fusion induced by Epstein-Barr virus replication in cl.S61 cells.
Sato
H
H
Department of Virology, Cancer Research Institute, Kanazawa, Japan.
Takimoto
T
T
Tanaka
S
S
Ogura
H
H
Shiraishi
K
K
Tanaka
J
J
D000212:Acyclovir / Q000494:pharmacology; D000956:Antigens, Viral / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000382:microbiology*; D002459:Cell Fusion; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000032:analysis; D003847:Deoxyglucose / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004854:Herpesvirus 4, Human / Q000187:drug effects / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D006801:Humans; D006822:Hybrid Cells; D007065:Idoxuridine / Q000494:pharmacology; D009303:Nasopharyngeal Neoplasms / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping; D014407:Tumor Cells, Cultured; D014779:Virus Replication
D000956:Antigens, Viral; D004279:DNA, Viral; D003847:Deoxyglucose; D007065:Idoxuridine; D000212:Acyclovir