Beta-globin enhancers target expression of a heterologous gene to erythroid tissues of transgenic mice.
To examine the role of human beta-globin enhancers in tissue-specific and developmental regulation, a hybrid beta-globin-simian virus 40 gene was analyzed in transgenic mice. A beta-globin DNA fragment containing two previously defined enhancers stimulated transcription from the simian virus 40 promoter in a tissue- and stage-specific pattern similar to that of the normal beta-globin gene. These results help to define the functions of beta-globin regulatory elements and suggest an approach for targeted expression of heterologous genes in erythroid cells in vivo.
Magram
J
J
Department of Genetics and Development, College of Physicians and Surgeons, Columbia University, New York, New York 10032.
Niederreither
K
K
Costantini
F
F
D000375:Aging / Q000097:blood; D000818:Animals; D005314:Embryonic and Fetal Development / Q000235:genetics; D004742:Enhancer Elements, Genetic / Q000502:physiology*; D004912:Erythrocytes / Q000502:physiology*; D005914:Globins / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D009928:Organ Specificity; D011401:Promoter Regions, Genetic / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics; D013539:Simian virus 40 / Q000235:genetics
D011993:Recombinant Fusion Proteins; D005914:Globins