title: Prostaglandin catabolism in fetal and maternal tissues--a study of 15-hydroxyprostaglandin dehydrogenase and delta 13 reductase with specific assay methods.
abstract: Recent studies have demonstrated that extraductal tissues such as lung are important sources of prostaglandin E2 which maintains the patency of ductus arteriosus in fetuses and prematurely-born infants. Also, organs such as lung are known to be active in the catabolism of PGE2. Earlier studies of enzymes involved in the catabolism of PGE2 such as 15-hydroxyprostaglandin dehydrogenase (15-PGDH) and delta 13 reductase all used non-specific methods. In the present report, we studied 15-PGDH in fetal and maternal rat lung, kidney, and fetal lamb lung, kidney and ductus arteriosus with the use of a specific substrate (15-S)-[15(3)H-PGE2]. In addition, we measured the activity of delta 13 reductase in these tissues by measuring the conversion of [1-14C]-15-keto PGE2 to [1-14C]-15-keto-13,14-dihydro PGE2. The results from these studies demonstrated that in fetal rat lung and kidney, 15-PGDH activities increased rapidly while delta 13 reductase remained unchanged during late gestation. Ductus arteriosus possessed little 15-PGDH activities. These results strongly suggest that extraductal regulation of PGE2 metabolism is important in determining ductal caliber in fetuses and prematurely delivered neonates.
authors.affiliation: Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis 55455.
authors.forename: M Y
authors.initials: MY
authors.lastname: Tsai
authors.forename: S
authors.initials: S
authors.lastname: Einzig
mesh_terms: D011452:15-Oxoprostaglandin 13-Reductase; D000818:Animals; D004373:Ductus Arteriosus / Q000201:enzymology*; D005260:Female; D005333:Fetus / Q000098:blood supply; D005865:Gestational Age; D006911:Hydroxyprostaglandin Dehydrogenases / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D008431:Maternal-Fetal Exchange; D010088:Oxidoreductases / Q000378:metabolism*; D011247:Pregnancy; D011453:Prostaglandins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012756:Sheep; D013379:Substrate Specificity
chemical_list: D011453:Prostaglandins; D010088:Oxidoreductases; D006911:Hydroxyprostaglandin Dehydrogenases; C035859:15-hydroxyprostaglandin dehydrogenase; D011452:15-Oxoprostaglandin 13-Reductase