Cloning of a polymorphic DNA fragment from the genome of Leishmania donovani.
Recombinant DNA clones, containing highly repetitive DNA sequences, have been isolated from a Leishmania donovani genomic DNA library prepared in the replacement vector lambda gt.WES.lambda B. Two clones, probably telomeric in location, have been characterised and show a restriction fragment size polymorphism. Evidence is presented which suggests that L. donovani is diploid for this cloned genomic locus.
Ellis
J
J
Wolfson Molecular Genetics Unit, Liverpool School of Tropical Medicine, U.K.
Knapp
T
T
Crampton
J
J
D000818:Animals; D001345:Autoradiography; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D015342:DNA Probes; D015246:Deoxyribonuclease EcoRI; D004587:Electrophoresis, Agar Gel; D007893:Leishmania donovani / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping
D015342:DNA Probes; D004247:DNA; D015246:Deoxyribonuclease EcoRI