Effects of Aroclor 1254 on dopamine and norepinephrine concentrations in pheochromocytoma (PC-12) cells.
Pheochromocytoma (PC-12) cells synthesize, store, release and metabolize dopamine (DA) and norepinephrine (NE) in a manner analogous to that observed in the mammalian central nervous system. These cells were used to develop and validate an alternate method to animal testing to assess the effects of a complex environmental mixture of polychlorinated biphenyls (Aroclor 1254) on cellular catecholamine function. Aroclor 1254, at concentrations of 1 to 100 ppm, significantly decreased cellular catecholamine concentrations after 6 hrs. Exposure at 100 ppm for periods of less than an hr increased cellular catecholamine concentrations while longer exposure times (i.e., 1 to 24 hr) decreased cellular catecholamine concentrations. This in vitro depletion of catecholamines is similar to that seen in vivo. Thus, PC-12 cells may be useful for neurochemical evaluation of neurotoxicants with particular reference to effects on catecholaminergic systems.
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Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509.
Brosch
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Bush
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Ritz
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Shain
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D000310:Adrenal Gland Neoplasms / Q000378:metabolism*; D000311:Adrenal Glands / Q000473:pathology; D000818:Animals; D001140:Aroclors / Q000633:toxicity*; D002250:Carbon Radioisotopes; D002395:Catecholamines / Q000378:metabolism; D020111:Chlorodiphenyl (54% Chlorine); D003470:Culture Media; D003847:Deoxyglucose / Q000493:pharmacokinetics; D004298:Dopamine / Q000378:metabolism*; D007424:Intracellular Fluid / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism*; D010673:Pheochromocytoma / Q000378:metabolism* / Q000473:pathology; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D051381:Rats; D014407:Tumor Cells, Cultured
D001140:Aroclors; D002250:Carbon Radioisotopes; D002395:Catecholamines; D003470:Culture Media; D020111:Chlorodiphenyl (54% Chlorine); D003847:Deoxyglucose; D011078:Polychlorinated Biphenyls; D004298:Dopamine; D009638:Norepinephrine