The side-chain conformations of N-acetyl-7-,8-,9-deoxy-, and -4,7-dideoxy-neuraminic acid and their effect on the activation of CTP:N-acylneuraminic acid cytidylyltransferase.
The conformations of the deoxy side-chain analogs of N-acetylneuraminic acid and of N-acetyl-4-deoxyneuraminic acid have been studied by n.m.r. spectroscopy, which allowed us to distinguish between local minima conformations suggested by hard-sphere calculations. The conformations were correlated with the activity with CTP: N-acetylneuraminic acid cytidylyltransferase (CMPsialate synthase; EC 2.7.7.43).
Christian
R
R
Institut für Chemie der Universität für Bodenkultur Wien, Austria.
Schreiner
E
E
Zbiral
E
E
Schulz
G
G
D002236:Carbohydrate Conformation; D003198:Computer Simulation; D004789:Enzyme Activation; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008958:Models, Molecular; D019158:N-Acetylneuraminic Acid / Q000031:analogs & derivatives*; D000216:N-Acylneuraminate Cytidylyltransferase / Q000378:metabolism*; D009713:Nucleotidyltransferases / Q000378:metabolism*; D012794:Sialic Acids / Q000494:pharmacology
D012794:Sialic Acids; C063095:N-acetyl-7-deoxyneuraminic acid; C063098:N-acetyl-9-deoxyneuraminic acid; C063092:N-acetyl-4,7-dideoxyneuraminic acid; C063097:N-acetyl-8-deoxyneuraminic acid; D009713:Nucleotidyltransferases; D000216:N-Acylneuraminate Cytidylyltransferase; D019158:N-Acetylneuraminic Acid