Inhibition of hydroxyl-radical-generated DNA degradation by metallothionein.
The effect of metallothionein (MT) on hydroxyl-radical-induced DNA damage was investigated in an in-vitro study. The degradation of DNA as demonstrated by agarose gel electrophoresis was inhibited by MT in a concentration-dependent manner. The degradation of DNA was almost completely inhibited by a concentration of 13 microM MT, whereas a concentration of 10 mM glutathione was needed to achieve the same protective effect.
Abel
J
J
Medical Institute of Environmental Hygiene, Department of Toxicology, Düsseldorf, F.R.G.
de Ruiter
N
N
D002374:Catalase / Q000494:pharmacology; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D004587:Electrophoresis, Agar Gel; D005609:Free Radicals; D005978:Glutathione / Q000494:pharmacology; D006878:Hydroxides / Q000037:antagonists & inhibitors*; D017665:Hydroxyl Radical; D008668:Metallothionein / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000494:pharmacology
D005609:Free Radicals; D006878:Hydroxides; D017665:Hydroxyl Radical; D004247:DNA; D008668:Metallothionein; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione