Human IgE and IgG antibodies to mosquito proteins detected by the immunoblot technique.
Immunoblot technique was used in this study to detect IgE and IgG antibodies in human sera against mosquito antigens. Mosquito proteins were separated on SDS-polyacrylamide gels and transferred electrophoretically to nitrocellulose papers. After incubation with sera from different individuals, the precipitated bands were analyzed with enzyme-labeled goat anti-human immunoglobulins. Distinct patterns of antigen were recognized by IgE and IgG antibodies.
Shen
H D
HD
Department of Medical Research, Veterans General Hospital, Taipei, Republic of China.
Chen
C C
CC
Chang
H N
HN
Chang
L Y
LY
Tu
W C
WC
Han
S H
SH
D000293:Adolescent; D000328:Adult; D000818:Animals; D000941:Antigens / Q000276:immunology; D009033:Culicidae / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis*; D008297:Male; D011506:Proteins / Q000276:immunology*
D000941:Antigens; D007074:Immunoglobulin G; D011506:Proteins; D007073:Immunoglobulin E