title: Lymphoid and mesenchymal tumors in transgenic mice expressing the v-fps protein-tyrosine kinase.
abstract: src, abl, and fps/fes are prototypes for a family of genes encoding nonreceptor protein-tyrosine kinases. The oncogenic potential of the v-fps protein-tyrosine kinase was investigated by introduction of the gag-fps coding sequence of Fujinami sarcoma virus into the mouse germ line. Transgenic mice with v-fps under the transcriptional control of a 5' human beta-globin promoter (GF) or with both 5' and 3' beta-globin regulatory sequences (GEF) were viable. Unexpectedly, both GF and GEF transgenes were expressed in a wide variety of tissues and induced a spectrum of benign and malignant tumors. These tumors, which included lymphomas, thymomas, fibrosarcomas, angiosarcomas, hemangiomas, and neurofibrosarcomas, developed with various frequencies after latent periods of 2 to 12 months. The majority of lymphoid neoplasms appeared to be of T-cell origin and were monoclonal, as judged by rearrangements of the T-cell receptor beta or immunoglobulin genes. Some tissues that expressed the v-fps oncogene, such as heart, brain, lung, and testes, developed no malignant tumors. The v-fps protein-tyrosine kinase therefore has a broad but not unrestricted range of oncogenic activity in cells of lymphoid and mesenchymal origin. The incomplete penetrance of the neoplastic phenotype and the monoclonality of lymphoid tumors suggest that tumor formation in v-fps mice requires genetic or epigenetic events in addition to expression of the P130gag-fps protein-tyrosine kinase.
authors.affiliation: Division of Molecular and Developmental Biology, Mt. Sinai Hospital Research Institute, Toronto, Ontario, Canada.
authors.forename: S P
authors.initials: SP
authors.lastname: Yee
authors.forename: D
authors.initials: D
authors.lastname: Mock
authors.forename: P
authors.initials: P
authors.lastname: Greer
authors.forename: V
authors.initials: V
authors.lastname: Maltby
authors.forename: J
authors.initials: J
authors.lastname: Rossant
authors.forename: A
authors.initials: A
authors.lastname: Bernstein
authors.forename: T
authors.initials: T
authors.lastname: Pawson
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D015870:Gene Expression; D005914:Globins / Q000235:genetics; D006391:Hemangioma / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D006801:Humans; D005256:Leukemia Virus, Feline / Q000235:genetics; D008223:Lymphoma / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009857:Oncogenes; D009928:Organ Specificity; D010957:Plasmids; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D012513:Sarcoma, Experimental / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D013945:Thymoma / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D013953:Thymus Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000473:pathology
chemical_list: D011518:Proto-Oncogene Proteins; C052602:proto-oncogene protein c-fes-fps; D005914:Globins; D011505:Protein-Tyrosine Kinases