Coxsackievirus-B3-induced myocarditis: virus receptor antibodies modulate myocarditis.
Two variants of coxsackievirus group B, type 3 (CVB3) differ in ability to induce myocarditis in Balb/cCUM mice. Infection with the highly pathogenic variant (CVB3M) stimulates autoimmunity to normal cardiocyte antigens, and tissue injury results primarily from an autoreactive cytolytic T lymphocyte (ACTL). Animals infected with the less pathogenic CVB3o variant do not develop ACTL, although CVB3o replicates to high titers in the heart and polyclonal neutralizing antisera fail to distinguish between the two variant virions. The present study uses two IgM mAb derived by fusing spleen cells from CVB3M-infected mice with NS-1 cells. These mAb investigate important differences between the virus variants that may explain why only selected infections trigger autoimmunity. mAb 8A6 is a virus-neutralizing antibody that prevents infection of HeLa cells and cultured cardiocytes by attaching to the virus. mAb 10A1 also interferes with infection but presumably reacts to the virus receptor on the susceptible cells and shows little or no binding to the virions. While 8A6 is equally effective in neutralizing both CVB3o and CVB3M, suggesting that antigenic epitopes on both variants are either identical or highly cross-reactive, 10A1 distinguishes between the variants, suggesting that the pathogenic and less pathogenic viruses use distinct cell surface receptors. Competitive binding studies using radiolabeled CVB3M and either of the unlabeled variants confirm this hypothesis. Both mAb effectively prevent CVB3M-induced cardiac damage in vivo. mAb 10A1 also inhibits autoreactive ACTL lysis of cardiocytes, indicating that the autoimmune effectors may recognize the virus receptor, and that the receptor utilized by a virus may prove important in triggering auto-sensitization.
Weller
A H
AH
University of Vermont, College of Medicine, Department of Pathology.
Simpson
K
K
Herzum
M
M
Van Houten
N
N
Huber
S A
SA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology; D000914:Antibodies, Viral / Q000502:physiology*; D000937:Antigen-Antibody Reactions; D001327:Autoimmune Diseases / Q000209:etiology / Q000276:immunology / Q000378:metabolism; D029822:Enterovirus B, Human / Q000276:immunology* / Q000378:metabolism / Q000472:pathogenicity; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D009205:Myocarditis / Q000209:etiology / Q000276:immunology* / Q000378:metabolism; D009206:Myocardium / Q000276:immunology / Q000378:metabolism; D011991:Receptors, Virus / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D011991:Receptors, Virus