Characterization of human T-cell lines harboring defective human immunodeficiency virus type 1.
Defective HIV-producing T-cell lines were subcloned from MT-4/HIVHTLV-IIIB' MOLT-4/HIVHTLV-IIIB, and H9/HIVHTLV-IIIB cell lines chronically infected with HIV. The NY-M10 cell line derived from MOLT-4/HIVHTLV-IIIB and the NY-H6 cell line derived from H9/HIVHTLV-IIIB produce defective HIV, which lacks the ability to infect human T-cell lines. NY-M10 cells retain the capacity to form multinucleated giant cells in cocultivation with HIV-uninfected CD4-positive cells. However, NY-H6 cells failed to fuse with CD4-positive cells. Electron microscopic analysis indicated that the defective HIV produced from NY-M10, like those reported previously, lacked the structure of the nucleocapsid, and the virion released from NY-H6 was indistinguishable from those of authentic HIV particles. Southern and Northern blotting analyses of NY-M10 and NY-H6 cleared that the genome of those defective viruses was not significantly deleted, suggesting minor mutation(s) should take place on the viral genome.
Hamamoto
Y
Y
Department of Virology and Parasitology, Yamaguchi University School of Medicine, Japan.
Takamatsu
K
K
Kobayashi
S
S
Yamaguchi
K
K
Yamamoto
N
N
Kobayashi
N
N
D002460:Cell Line; D005434:Flow Cytometry; D015686:Gene Products, env / Q000276:immunology; D015683:Gene Products, gag / Q000276:immunology; D015488:HIV Antigens / Q000276:immunology; D016655:HIV Core Protein p24; D015699:HIV Envelope Protein gp120 / Q000276:immunology; D019302:HIV Envelope Protein gp160; D015497:HIV-1 / Q000276:immunology / Q000648:ultrastructure*; D006801:Humans; D015151:Immunoblotting; D008561:Membrane Fusion; D011498:Protein Precursors / Q000276:immunology; D015531:Radioimmunoprecipitation Assay; D013601:T-Lymphocytes / Q000382:microbiology*; D014758:Viral Core Proteins / Q000276:immunology
D015686:Gene Products, env; D015683:Gene Products, gag; D015488:HIV Antigens; D016655:HIV Core Protein p24; D015699:HIV Envelope Protein gp120; D019302:HIV Envelope Protein gp160; D011498:Protein Precursors; D014758:Viral Core Proteins