A liver-specific enhancer in the core promoter region of human hepatitis B virus.
An 88-base pair fragment in the core promoter of the human hepatitis B virus (HBV) contains a functional promoter and a strong liver-specific enhancer. This enhancer functions in human hepatoma cells, where it is much more active than the previously described HBV enhancer in stimulating expression of the linked bacterial chloramphenicol acetyltransferase gene expressed from heterologous promoters. Studies of the role of this enhancer-promoter in HBV may help to clarify mechanisms of gene expression in cells infected with HBV and the role of the virus in the pathogenesis of hepatitis and hepatocellular carcinoma.
Yee
J K
JK
Department of Pediatrics, School of Medicine, University of California, San Diego, La Jolla 92093.
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D002872:Chromosome Deletion; D004742:Enhancer Elements, Genetic; D005814:Genes, Viral; D006515:Hepatitis B virus / Q000235:genetics*; D008099:Liver / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D011401:Promoter Regions, Genetic; D018139:Simplexvirus / Q000201:enzymology / Q000235:genetics; D013937:Thymidine Kinase / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection; D015678:Viral Structural Proteins / Q000235:genetics
D015678:Viral Structural Proteins; D015500:Chloramphenicol O-Acetyltransferase; D013937:Thymidine Kinase