Comparison of diagnostic tests for the detection of equine infectious anemia antibody.
Two diagnostic tests are approved for detecting antibody to equine infectious anemia virus: the agar-gel immunodiffusion (AGID) test and the competitive enzyme-linked immunosorbent assay (ELISA). A total of 420 sera from National Veterinary Services Laboratories check sets were tested with the AGID and competitive ELISA. A 100% correlation was obtained. The AGID and competitive ELISA were further used to test difficult samples with low levels of equine infectious anemia antibody (weak positives). A third test (Western blot) was also used with these weak positive samples to resolve any discordant results.
Matsushita
T
T
Syngene Products and Research, Fort Collins, CO 80524.
Hesterberg
L K
LK
Porter
J P
JP
Smith
B J
BJ
Newman
L E
LE
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D015153:Blotting, Western / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005779:Immunodiffusion / Q000662:veterinary*; D004860:Infectious Anemia Virus, Equine / Q000276:immunology*; D011237:Predictive Value of Tests
D000914:Antibodies, Viral