Enhancement of primary and secondary immune responses by interferon-gamma.
We have investigated the effects of interferon-gamma (IFN-gamma) administered with "G" glycoprotein of vesicular stomatitis virus (VSV), on the neutralizing antibody response. Treatment of mice or cattle with recombinant DNA-derived IFN-gamma at the time of primary immunization with "G" glycoprotein enhanced the secondary virus-neutralizing antibody response that followed a booster administration of the same antigen without IFN-gamma treatment. Enhancement was statistically significant, and occurred at relatively low doses of IFN-gamma in the absence of any additional adjuvants. Cattle treated with IFN-gamma at the time of primary immunization were also more resistant to VSV challenge than those immunized without IFN-gamma treatment. Such treatment in conjunction with primary immunization may therefore provide a practical means of enhancing protection from viral challenge without inflammatory adjuvants or boosters.
Yilma
T
T
Department of Veterinary Microbiology and Immunology, University of California, Davis 95616.
Owens
S
S
Fennie
E H
EH
Anderson
K P
KP
D000276:Adjuvants, Immunologic; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D002417:Cattle; D005260:Female; D007114:Immunization; D007371:Interferon-gamma / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D008562:Membrane Glycoproteins; D051379:Mice; D008811:Mice, Inbred DBA; D009500:Neutralization Tests; D011994:Recombinant Proteins; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology; D014759:Viral Envelope Proteins / Q000008:administration & dosage / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; C028391:G protein, vesicular stomatitis virus; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; D007371:Interferon-gamma