Benzodiazepine receptors in isolated adrenal glomerulosa cells.
In our experiments the isolated rat adrenal glomerulosa cells displayed peripheral-type benzodiazepine receptors, which could bind to [3H] PK11195 with an apparent equilibrium dissociation constant (KD) of 9.4 +/- 2.8 nmol/L and a maximal binding capacity (Bmax) of 5.6 +/- 1.8 pmol/10(6) cells. The effects of five ligands: PK11195, Ro5-4846, flunitrazepam, diazepam and clonazepam on aldosterone secretion responses of isolated glomerulosa cells to angiotensin II or extracellular potassium ions were observed. The logarithm of EO50 for these ligands as stimulators was well correlated with the logarithm of their Ki value for [3H] PK11195 binding, suggesting that the stimulative effects might be mediated by the benzodiazepine receptor in isolated glomerulosa cells.
Song
L C
LC
Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing.
Zhou
T C
TC
D000450:Aldosterone / Q000378:metabolism; D000818:Animals; D001570:Benzodiazepinones / Q000494:pharmacology; D000242:Cyclic AMP / Q000096:biosynthesis; D066298:In Vitro Techniques; D007546:Isoquinolines / Q000494:pharmacology; D008024:Ligands; D008297:Male; D011485:Protein Binding / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D011963:Receptors, GABA-A / Q000032:analysis* / Q000187:drug effects; D015384:Zona Glomerulosa / Q000166:cytology* / Q000378:metabolism
D001570:Benzodiazepinones; D007546:Isoquinolines; D008024:Ligands; D011963:Receptors, GABA-A; C028513:4'-chlorodiazepam; D000450:Aldosterone; D000242:Cyclic AMP; C037850:PK 11195