Size-exclusion chromatography of DNA restriction fragments. Fragment length determinations and a comparison with the behaviour of proteins in size-exclusion chromatography.
Size-exclusion chromatography of double-stranded DNA restriction fragments on Superose 6 is shown to be an accurate method for chain length determination of unknown DNA. Ionic interaction was observed between DNA and the gel matrix but was easily prevented by the addition of 0.15-0.2 M sodium chloride to the eluent. Compared to protein, the selectivity curve of DNA fragments was found to be steeper reflecting the different chromatographic behaviour of rod-like and globular molecules. The relationships between the selectivity curves of DNA and protein were similar on Superose 6 and on Sephacryl S-500.
Ellegren
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Pharmacia LKB Biotechnology, Uppsala, Sweden.
Låås
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D002850:Chromatography, Gel; D004247:DNA / Q000032:analysis*; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011506:Proteins / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D011506:Proteins; D004247:DNA