Requirement for lipopolysaccharide for enhanced in vitro superoxide producing competence in macrophages from normal and malaria (Plasmodium chabaudi) infected mouse spleen.
Nucleated cells from mouse spleens were suspended in RPMI-1640 medium and plated in polystyrene tissue culture dishes with or without lipoplysaccharide (LPS, endotoxin) and incubated at 37 degrees C for 3 h in a humid incubator under 5% CO2. Adherent spleen cells obtained from uninfected control or Plasmodium chabaudi-infected mice showed negligible superoxide (O2) production in vitro, in response to triggering with phorbol myristate acetate (PMA). However, O2 release increased in a concentration-dependent fashion as LPS was introduced above a threshold of about 10 ng/ml. This LPS dependence is at least 100 times more than that required by mouse peritoneal macrophages and 10(6) times greater than the requirement reported for human monocytes. These findings emphasize the differences among the three mononuclear cells, and also suggest that LPS could prove useful in the isolation and culture of other metabolically competent cells.
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Department of Biochemistry, University of Nigeria, Nsukka.
D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D005260:Female; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D008288:Malaria / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008863:Microspheres; D010587:Phagocytosis; D013154:Spleen / Q000166:cytology / Q000187:drug effects*; D013481:Superoxides / Q000378:metabolism*
D008070:Lipopolysaccharides; D013481:Superoxides