title: Yeast intrachromosomal recombination: long gene conversion tracts are preferentially associated with reciprocal exchange and require the RAD1 and RAD3 gene products.
abstract: A yeast intrachromosomal recombination system based on an inverted repeat has been designed to examine mitotic gene conversion tract length and the association of crossing over with gene conversion as a function of the conversion tract length. Short conversion tracts are found to be preferentially noncrossover while conversion tracts longer than 1.16 kb show a 50% association with crossover. Mutation in the excision repair gene RAD1 leads to a reduction in conversion tracts of at least 1.16 kb and a reduction in crossovers associated with conversion, regardless of the length of the conversion tract. Mutation in the excision repair gene RAD3, which encodes a DNA helicase, also leads to a reduction in conversion tracts of at least 1.16 kb, but has no effect on the frequency of associated crossovers. The roles of RAD1 and RAD3 in recombination are discussed.
authors.affiliation: Department of Biochemistry, New York University Medical Center, New York.
authors.forename: A
authors.initials: A
authors.lastname: Aguilera
authors.forename: H L
authors.initials: HL
authors.lastname: Klein
mesh_terms: D007446:Chromosome Inversion; D003434:Crossing Over, Genetic; D004260:DNA Repair; D004250:DNA Topoisomerases, Type II / Q000502:physiology; D005785:Gene Conversion; D005800:Genes, Fungal; D011995:Recombination, Genetic; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D004250:DNA Topoisomerases, Type II