Induced transposition of Ds by a stable Ac in crosses of transgenic tobacco plants.
A two component maize transposon system comprising a stable but trans-active Ac element and a cis responsive Ds element has been established in transgenic tobacco. The development of this system is desirable for the realization of a gene tagging strategy employing these elements in heterologous plant species. Transgenic tobacco with a single transposed Ac element (Ac-18) which has sustained a 4 bp terminal deletion has been identified. Transposase activity of the stable Ac-18 was demonstrated in a tissue culture assay as well as in transgenic plants. When plants containing Ac-18 were crossed with transgenic plants carrying a Ds element, 25%-50% of the F1 progeny showed trans-activation of Ds transposition. Analysis of DNA of several F1 plants showed that each plant displayed a unique pattern of Ds transposition to new chromosomal sites.
Hehl
R
R
USDA-ARS Plant Gene Expression Center, Albany CA 94710.
Baker
B
B
D015139:Blotting, Southern; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D004247:DNA / Q000235:genetics; D004251:DNA Transposable Elements; D004351:Drug Resistance / Q000235:genetics; D007612:Kanamycin / Q000494:pharmacology; D009713:Nucleotidyltransferases / Q000378:metabolism; D010947:Plants, Toxic; D015183:Restriction Mapping; D014026:Nicotiana / Q000235:genetics*; D014170:Transformation, Genetic; D019895:Transposases; D003313:Zea mays / Q000235:genetics*
D004251:DNA Transposable Elements; D007612:Kanamycin; D004247:DNA; D009713:Nucleotidyltransferases; D019895:Transposases