Location and molecular cloning of the structural gene for the deoxyguanosine triphosphate triphosphohydrolase of Escherichia coli.
The structural gene for deoxyguanosine triphosphate triphosphohydrolase (dGTPase) (EC 3.1.5.1) and its regulator, optA, have been located on a lambda phage carrying a 17.5kb Escherichia coli DNA insert. The DNA fragment has been excised and ligated into pBR325 and also transferred to another lambda vector. From the results of transduction and transformation experiments, we find that the structural gene for dGTPase is very closely linked to optA and dapD, which locates it at approximately 3.6 minutes on the genetic map of E. coli K12. We propose the mnemonic dgt as the designation for the structural gene for this enzyme.
Quirk
S
S
McCollum-Pratt Institute, Baltimore, Maryland.
Seto
D
D
Bhatnagar
S K
SK
Gauss
P
P
Gold
L
L
Bessman
M J
MJ
D010582:Bacteriophage lambda; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D005796:Genes; D005822:Genetic Vectors; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics* / Q000378:metabolism; D014161:Transduction, Genetic
D010744:Phosphoric Monoester Hydrolases