HBV-DNA hybridization in hepatocellular carcinoma associated with alcohol in Japan.
To clarify the role of the hepatitis B virus (HBV) in hepatocellular carcinoma (HCC) associated with alcohol consumption, HBV-DNA in the liver of 19 patients with HCC were investigated. HBV-DNA was examined by Southern blot hybridization. HBV-DNA was integrated into tumor cells from five out of six (83%) patients with HCC associated with HBs antigen (HBsAg)-positive post-hepatitic liver cirrhosis (LC), but this was not related to the history of alcohol intake. In 13 HCC patients of HBsAg-negative alcoholic LC, HBV-DNA integration was not detected in any patient. These findings suggest that HBV does not play a major role in the pathogenesis of HCC in HBsAg-negative alcoholics in Japan.
Horiike
N
N
Third Department of Internal Medicine, Ehime University School of Medicine, Japan.
Michitaka
K
K
Onji
M
M
Murota
T
T
Ohta
Y
Y
D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000209:etiology* / Q000382:microbiology; D004279:DNA, Viral / Q000032:analysis*; D006511:Hepatitis B Antigens / Q000032:analysis; D006515:Hepatitis B virus / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D006801:Humans; D007564:Japan; D008099:Liver / Q000382:microbiology; D008104:Liver Cirrhosis, Alcoholic / Q000150:complications* / Q000382:microbiology; D008113:Liver Neoplasms / Q000209:etiology* / Q000276:immunology / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization
D004279:DNA, Viral; D006511:Hepatitis B Antigens