title: Neuroendocrine, sympathetic and metabolic responses induced by interleukin-1.
abstract: Effects on turnover of vasopressin (AVP) in the hypothalamus and on secretion of pituitary hormones, catecholamines and insulin after intraperitoneal injection of recombinant interleukin-1 (beta) (IL-1) were investigated in male wistar rats. Intraperitoneal administration of IL-1 in a dose (1 microgram) that maximally activated pituitary-adrenal activity failed to alter plasma concentrations of prolactin, luteinizing hormone and melanocyte-stimulating hormone. Rats chronically cannulated in the right jugular veins showed a time-related increase in plasma corticosterone concentrations in response to intraperitoneal administration of IL-1 that lasted up to 4 h. In the same rats, plasma epinephrine (E) and norepinephrine (NE) concentrations were only slightly elevated (2-fold increase) at 30 min and at 1 h after IL-1 administration. Unlike in endotoxin-resistant C3H/HeJ mice, where IL-1 induces hypoglycemia, IL-1 did not affect plasma concentrations of glucose and insulin in Wistar rats. In the zona externa of the median eminence, IL-1 stimulated corticotropin-releasing factor (CRF) turnover at an approximate rate of 15%/h, but did not cause a concomitant change in AVP turnover as can be observed after insulin-induced hypoglycemia. Since half of the hypothalamic CRF neurons have been shown to costore AVP, the data favor the view of a selective effect of IL-1 on a subtype of CRF neurons. We conclude that pituitary-adrenal activation in response to Il-1 is caused by CRF secretion from a subtype of CRF neurons (not storing AVP) in the rat hypothalamus.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Pharmacology, Medical Faculty, Free University, Amsterdam, The Netherlands.
authors.forename: F
authors.initials: F
authors.lastname: Berkenbosch
authors.forename: D E
authors.initials: DE
authors.lastname: de Goeij
authors.forename: A D
authors.initials: AD
authors.lastname: Rey
authors.forename: H O
authors.initials: HO
authors.lastname: Besedovsky
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000097:blood; D000818:Animals; D001786:Blood Glucose; D002395:Catecholamines / Q000097:blood*; D003592:Cytophotometry; D007150:Immunohistochemistry; D007274:Injections, Intraperitoneal; D007328:Insulin / Q000097:blood; D007375:Interleukin-1 / Q000494:pharmacology*; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D009074:Melanocyte-Stimulating Hormones / Q000097:blood; D010902:Pituitary Gland / Q000378:metabolism; D010907:Pituitary Hormones / Q000097:blood*; D011388:Prolactin / Q000097:blood; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D001786:Blood Glucose; D002395:Catecholamines; D007328:Insulin; D007375:Interleukin-1; D010907:Pituitary Hormones; D000324:Adrenocorticotropic Hormone; D011388:Prolactin; D007986:Luteinizing Hormone; D009074:Melanocyte-Stimulating Hormones