Analysis of the cis-acting requirements for germ-line-specific splicing of the P-element ORF2-ORF3 intron.
P-element transposition is limited to the germ line because the element's third intron is only spliced in germ line cells. We show that a 240-bp fragment containing this 190-bp intron can confer germ line specificity when placed in the context of another gene. We find that the cis-acting regulatory sequences required for germ line regulation map near to, but not at, the 5' or 3' splice junctions.
Laski
F A
FA
Department of Biochemistry, University of California, Berkeley 94720.
Rubin
G M
GM
D000818:Animals; D001483:Base Sequence; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics; D005854:Germ Cells; D007438:Introns; D008969:Molecular Sequence Data; D009154:Mutation; D009713:Nucleotidyltransferases / Q000378:metabolism; D010957:Plasmids; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D014170:Transformation, Genetic; D019895:Transposases; D001616:beta-Galactosidase
D004251:DNA Transposable Elements; D012333:RNA, Messenger; D009713:Nucleotidyltransferases; D019895:Transposases; D001616:beta-Galactosidase