Sheep platelets as a model for human platelets: evidence for specific PAF (platelet activating factor) receptors.
In a number of animals, the platelet response to Platelet Activating Factor (PAF) has been shown to differ considerably from that in humans. However, aggregation, release and particularly shape change were quite similar for human and sheep platelets. In this study, aggregation and shape change analysis were used to measure the response of sheep platelets to various synthetic analogs of PAF. Response is greatly reduced with no alkyl group in position 2 of PAF and decreases progressively as the number of carbons of the alkyl gets larger than three. A reduction of activity is also seen as the ether linkage at position 1 of PAF is replaced by an ester linkage. These changes are indicative of a specific membrane receptor for PAF in sheep platelets and confirm the usefulness of sheep platelets as a model for PAF-platelet interaction in humans.
Slattery
C W
CW
Department of Biochemistry, Loma Linda University School of Medicine, CA 92350.
Beaumont
D O
DO
D000818:Animals; D001792:Blood Platelets / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D010972:Platelet Activating Factor / Q000378:metabolism* / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects*; D010980:Platelet Membrane Glycoproteins; D011956:Receptors, Cell Surface / Q000032:analysis*; D043562:Receptors, G-Protein-Coupled; D012756:Sheep / Q000097:blood*; D013045:Species Specificity; D013329:Structure-Activity Relationship
D010972:Platelet Activating Factor; D010980:Platelet Membrane Glycoproteins; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; C039351:platelet activating factor receptor