Biosynthesis of 130-kilodalton mosquito larvicide in the cyanobacterium Agmenellum quadruplicatum PR-6.
The 130-kilodalton mosquito larvicidal gene, cloned from Bacillus thuringiensis var. israelensis, was introduced into the cyanobacterium Agmenellum quadruplicatum PR-6 by plasmid transformation. Transformed cells synthesized 130-kilodalton delta-endotoxin protein and showed mosquito larvicidal activity. Results demonstrate a potential use of a cyanobacterium for biological control of mosquitoes.
Angsuthanasombat
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Center for Molecular Genetics-Genetic Engineering, Mahidol University, Bangkok, Thailand.
Panyim
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D000330:Aedes / Q000382:microbiology*; D000818:Animals; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000235:genetics / Q000378:metabolism*; D004731:Endotoxins / Q000096:biosynthesis* / Q000633:toxicity; D005822:Genetic Vectors; D006460:Hemolysin Proteins; D007814:Larva / Q000187:drug effects / Q000382:microbiology; D008970:Molecular Weight; D010957:Plasmids; D014170:Transformation, Genetic
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis