An improved method for Lowicryl K4M electron microscopic embedding of brain tissue.
The Lowicryl K4M low-temperature embedding technique was modified for brain tissue to facilitate antigen localization in neuronal structures by using postembedding immunoelectron microscopy. Rat brains fixed by perfusion with a mixture of 1% glutaraldehyde and 1% paraformaldehyde, were sectioned into 200-microns-thick coronal sections, and the appropriate area of the sections further microdissected into small samples (200 x 700 x 700 microns). These samples were processed for K4M embedding using prolonged incubation times at each step. This resulted in a homogeneously infiltrated tissue with good preservation of ultrastructure and antigen reactivity. Despite the lack of osmium fixation, the morphology of the synapses was clearly identifiable.
Kiss
J Z
JZ
Institute of Histology and Embryology, University of Geneva Medical School, Switzerland.
D000180:Acrylic Resins; D000818:Animals; D001921:Brain / Q000378:metabolism / Q000648:ultrastructure*; D007150:Immunohistochemistry / Q000379:methods*; D008297:Male; D008854:Microscopy, Electron / Q000379:methods*; D008867:Microtomy / Q000379:methods*; D018377:Neurotransmitter Agents / Q000378:metabolism*; D051381:Rats
D000180:Acrylic Resins; D018377:Neurotransmitter Agents; C037883:Lowicryl 4KM resin