title: Multiple forms of calpastatin in pig brain.
abstract: Pig brain was found to contain two calpain-specific, heat-stable inhibitory fractions which could be separated by DEAE-cellulose chromatography. CS-0.1, which was eluted from the column at 0.1 M NaCl, was identified as an ordinary, well-known calpastatin. CS-0.2, eluted at 0.2 M NaCl, was different from CS-0.1 in that it inhibited calpain 1 more strongly than calpain II and that it did not cross-react with anti-calpastatin antibodies. Partial purification indicated that CS-0.2 contained inhibitor proteins smaller than ordinary calpastatin, but whether they are the products derived from CS-0.1 or entirely different genetic products has not yet been determined.
authors.affiliation: Department of Clinical Science and Laboratory Medicine, Kyoto University Faculty of Medicine, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Takano
authors.forename: T
authors.initials: T
authors.lastname: Hamakubo
authors.forename: Y
authors.initials: Y
authors.lastname: Kawatani
authors.forename: M
authors.initials: M
authors.lastname: Ueda
authors.forename: R
authors.initials: R
authors.lastname: Kannagi
authors.forename: T
authors.initials: T
authors.lastname: Murachi
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001923:Brain Chemistry; D002135:Calcium-Binding Proteins / Q000276:immunology; D002154:Calpain / Q000037:antagonists & inhibitors*; D002852:Chromatography, Ion Exchange; D003429:Cross Reactions; D015853:Cysteine Proteinase Inhibitors / Q000276:immunology / Q000302:isolation & purification*; D013552:Swine
chemical_list: D002135:Calcium-Binding Proteins; D015853:Cysteine Proteinase Inhibitors; C033668:calpastatin; D002154:Calpain