title: Selective killing of CD4+ and CD8+ cells with immunotoxins containing saporin.
abstract: Saporin, a ribosome-inactivating protein from the seeds of Saponaria officinalis, was covalently linked to an anti-CD4 monoclonal antibody. The resulting immunotoxin at 10(-9)M concentration was toxic to CD4+ lymphocytes without affecting other cells. Selective elimination of CD4+ and CD8+ cells was also obtained with murine monoclonal anti-CD4 and anti-CD8 antibodies and an immunotoxin consisting of saporin linked to an anti-mouse IgG antibody.
authors.affiliation: Department of Experimental Pathology, University of Bologna, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Barbieri
authors.forename: A
authors.initials: A
authors.lastname: Bolognesi
authors.forename: A
authors.initials: A
authors.lastname: Dinota
authors.forename: D A
authors.initials: DA
authors.lastname: Lappi
authors.forename: M
authors.initials: M
authors.lastname: Soria
authors.forename: P L
authors.initials: PL
authors.lastname: Tazzari
authors.forename: F
authors.initials: F
authors.lastname: Stirpe
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D000922:Immunotoxins / Q000494:pharmacology*; D066298:In Vitro Techniques; D009699:N-Glycosyl Hydrolases; D010940:Plant Proteins / Q000494:pharmacology*; D054789:Ribosome Inactivating Proteins, Type 1; D000076985:Saporins; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000922:Immunotoxins; D010940:Plant Proteins; D054789:Ribosome Inactivating Proteins, Type 1; D009699:N-Glycosyl Hydrolases; D000076985:Saporins