title: Effect of some penicillins on the sensitivity of limulus amoebocyte lysate test.
abstract: A group of penicillins have been tested for their effect on the sensitivity of limulus amebocyte lysate test (LAL). Cloxacillin, cephalothin and cefuroxime inhibited the gel formation at concentrations above 2 mg mL-1, while ampicillin, methicillin and mecillinam showed no inhibitory effects upto concentrations of 10 mg mL-1. For benzylepenicillin the maximum non-inhibitory concentration was 10,000 units mL-1. However, the dilutions required to overcome inhibition were within the limits of maximum valid dilutions computed for each product showing that LAL test is valid for these products at their non-inhibitory concentrations.
authors.affiliation: Drug Control and Registration Centre, Ministry of Health, Safat, Kuwait.
authors.forename: I J
authors.initials: IJ
authors.lastname: al-Khalifa
authors.forename: N V
authors.initials: NV
authors.lastname: Naidu
authors.forename: V R
authors.initials: VR
authors.lastname: Nallari
authors.forename: H I
authors.initials: HI
authors.lastname: Raut
mesh_terms: D000818:Animals; D004731:Endotoxins / Q000032:analysis; D008033:Limulus Test; D010406:Penicillins / Q000494:pharmacology*; D011817:Rabbits
chemical_list: D004731:Endotoxins; D010406:Penicillins