Cytoskeletal reorganization during electric-field-induced fusion of Chinese hamster ovary cells grown in monolayers.
Mammalian cells were shown to fuse after direct electric pulsation of the plated cells in culture. The extent of fusion was controlled by the duration of the post-pulse incubation. Formation of polynucleated cells was slow, even at 37 degrees C. Pre-pulse incubation with colchicine increased the fusion yield slightly. Cytoskeletal organization during the post-pulse incubation was observed using immunofluorescence techniques. Microfilaments were unaffected, but microtubules disappeared during the first minutes following the pulse, and then reformed on subsequent incubation.
Blangero
C
C
Centre de Biochimie et de Génétique cellulaires du CNRS, Toulouse, France.
Rols
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MP
Teissié
J
J
D008841:Actin Cytoskeleton / Q000648:ultrastructure; D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D002459:Cell Fusion; D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure; D003078:Colchicine / Q000494:pharmacology; D006224:Cricetinae; D003412:Cricetulus; D003571:Cytochalasin B / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects / Q000648:ultrastructure*; D004560:Electricity; D005260:Female; D005455:Fluorescent Antibody Technique; D008870:Microtubules / Q000648:ultrastructure; D010053:Ovary; D017239:Paclitaxel; D013696:Temperature; D013997:Time Factors
D000470:Alkaloids; D003571:Cytochalasin B; D017239:Paclitaxel; D003078:Colchicine