title: Rat mesangial cells actively produce phosphatidylinositol-anchored Thy-1.
abstract: We provide evidence that the mesangial cells of rat kidney glomeruli express Thy-1 as a phosphatidylinositol-anchored protein. Both the mesangial area of kidney, examined in tissue sections, and mesangial cells maintained in culture for more than 3 months, showed prominent immunofluorescence staining with an anti-Thy-1 monoclonal antibody (OX7); this staining was almost completely abolished by pretreating kidney sections or mesangial cells with the phosphatidylinositol-specific enzyme, phospholipase C. By Northern blotting, mesangial cells were shown to express mRNA of an appropriate size, hybridizing to a mouse Thy-1.1-specific probe.
authors.affiliation: Department of Immunology, Nagoya University School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Miyata
authors.forename: K
authors.initials: K
authors.lastname: Isobe
authors.forename: R
authors.initials: R
authors.lastname: Inagi
authors.forename: R
authors.initials: R
authors.lastname: Taguchi
authors.forename: H
authors.initials: H
authors.lastname: Ikezawa
authors.forename: I
authors.initials: I
authors.lastname: Takai
authors.forename: Y
authors.initials: Y
authors.lastname: Fujita
authors.forename: T
authors.initials: T
authors.lastname: Iwamoto
authors.forename: T
authors.initials: T
authors.lastname: Hasegawa
authors.forename: O
authors.initials: O
authors.lastname: Oda
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D002478:Cells, Cultured; D005920:Glomerular Mesangium / Q000276:immunology*; D010716:Phosphatidylinositols / Q000276:immunology*; D051381:Rats; D018800:Thy-1 Antigens
chemical_list: D000954:Antigens, Surface; D010716:Phosphatidylinositols; D018800:Thy-1 Antigens