title: Induction of type II 5'-deiodinase activity in cultured rat astroglial cells by 12-O-tetradecanoylphorbol-13-acetate: dependence on glucocorticoids.
abstract: The effect of an activator of protein kinase C, 12-O-tetradecanoylphorbol-13-acetate (TPA), on the 5'-deiodinase (5'D) activity was studied in rat astroglial cells cultured in chemically defined medium. TPA promoted a large increase in the type II 5'D activity, which was maximal 5-10 h after addition of TPA and then declined to the basal level at 24 h. The optimal TPA concentration was 10(-7) M. TPA and 8-Br-cAMP, an other inducer of 5'D activity were antagonist. Otherwise, TPA stimulated 5'D activity only in the presence of glucocorticoids at concentrations from 10(-8) M to 10(-4) M. Glucocorticoids alone induced a slight increase in 5'D activity. These data indicate that protein kinase C contributes to the control of 5'D activity in astroglial cells and that its action is dependent on glucocorticoids.
authors.affiliation: Unité de Recherche sur la Glande Thyroïde et la Régulation Hormonale (U.96) de l'Institut National de la Santé et de la Recherche Médicale, Le Kremlin-Bicêtre, France.
authors.forename: F
authors.initials: F
authors.lastname: Courtin
authors.forename: F
authors.initials: F
authors.lastname: Chantoux
authors.forename: J M
authors.initials: JM
authors.lastname: Gavaret
authors.forename: D
authors.initials: D
authors.lastname: Toru-Delbauffe
authors.forename: C
authors.initials: C
authors.lastname: Jacquemin
authors.forename: M
authors.initials: M
authors.lastname: Pierre
mesh_terms: D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000187:drug effects / Q000201:enzymology*; D001921:Brain / Q000201:enzymology; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology; D004790:Enzyme Induction; D006854:Hydrocortisone / Q000494:pharmacology; D007453:Iodide Peroxidase / Q000096:biosynthesis*; D007527:Isoenzymes / Q000096:biosynthesis*; D007700:Kinetics; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
chemical_list: D007527:Isoenzymes; D015124:8-Bromo Cyclic Adenosine Monophosphate; D003907:Dexamethasone; D000242:Cyclic AMP; D007453:Iodide Peroxidase; D013755:Tetradecanoylphorbol Acetate; D006854:Hydrocortisone