title: Antibody response of pseudorabies virus subunit-vaccinated pigs to viral nucleocapsid proteins following low-dose virus challenge of immunity.
abstract: The antibody response to pseudorabies virus nucleocapsid proteins (NCP) was evaluated by the western immunoblot analysis before and after challenge of immunity by nasal inoculation of 10(2.3) plaque-forming units of virus in 10 pigs that had been vaccinated with pseudorabies virus envelope glycoproteins. Antibody to 5 NCP with molecular mass of 140, 63, 41, 34, and 23 kD was first detected in vaccinated and nonvaccinated pigs on day 14 after challenge of immunity. Antibody to 2 of the 5 NCP continued to be detected through day 113 in 9 of 10 vaccinated pigs. Beyond day 32, antibody to NCP was not detected in 1 vaccinated pig. The 23-, 34-, and 41-kD proteins were the most immunogenic. Antibody to each of these proteins was first detected on day 14 in 10, 10, and 8 pigs, respectively. Seven, 6, and 8 pigs, respectively, were antibody-positive for these proteins on day 113. The 140- and 63-kD proteins were the least immunogenic. Antibody to these proteins was detected in 8 and 9 pigs, respectively, on day 14, and in 4 and 5 pigs, respectively, on day 113. Chi-square analysis for dependency indicated that the antibody response to the 140- and 63-kD proteins was interdependent. These results suggested that combinations of NCP may be useful as nonvaccine diagnostic antigens.
authors.affiliation: Department of Veterinary Microbiology, Iowa State University, Ames 50011.
authors.forename: M J
authors.initials: MJ
authors.lastname: McGinley
authors.forename: K B
authors.initials: KB
authors.lastname: Platt
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D015153:Blotting, Western; D002213:Capsid / Q000276:immunology; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D008970:Molecular Weight; D011557:Pseudorabies / Q000276:immunology*; D013552:Swine; D013553:Swine Diseases / Q000276:immunology*; D014611:Vaccination / Q000662:veterinary; D014758:Viral Core Proteins / Q000276:immunology; D014765:Viral Vaccines / Q000032:analysis / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D014758:Viral Core Proteins; D014765:Viral Vaccines