Campylobacter species identification based on polymorphism of DNA encoding rRNA.
Total DNA from five Campylobacter species was digested with a mixture of XhoI and BglII restriction endonucleases and analyzed by Southern hybridization by using a probe complementary to the DNA coding for the 16S rRNA. Each of the Campylobacter species, including C. jejuni, C. coli, C. laridis, C. fetus, and C. upsaliensis, displayed a characteristic pattern. Although some bands may be common to different species, the simplicity of the hybridization pattern enabled us to discriminate among the different species of Campylobacter.
Moureau
P
P
Unité de Microbiologie, Université Catholique de Louvain, Brussels, Belgium.
Derclaye
I
I
Gregoire
D
D
Janssen
M
M
Cornelis
G R
GR
D001426:Bacterial Proteins; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002167:Campylobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific; D006801:Humans; D009693:Nucleic Acid Hybridization; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012335:RNA, Ribosomal / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D015183:Restriction Mapping
D001426:Bacterial Proteins; D015342:DNA Probes; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; D012336:RNA, Ribosomal, 16S; C024404:BglII endonuclease; C034510:CTCGAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific