Concentration of poliovirus type I from cow milk using an adsorption-elution method.
Cow milk was experimentally contaminated with a vaccine strain of poliovirus type I. A concentration procedure was utilized to assay for the virus in a milk sample using adsorption on aluminium sulphate at pH 4.5-5.5, aluminium sulphate concentration being 0.15 g. 1(-1), followed by elution with 0.1 M Na2HPO4 pH 9.5 and subsequent detection in cultured VERO cells (kidney cells from the monkey Cercopithecus aethiops). The yield of the virus ranged from 14 to 58% of the inoculated amount, the mean value being 27%.
Hucko
M
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Research Institute of Preventive Medicine, Bratislava, Czechoslovakia.
D000327:Adsorption; D000534:Alum Compounds; D000818:Animals; D008892:Milk / Q000382:microbiology*; D017955:Poliovirus / Q000302:isolation & purification*; D013048:Specimen Handling; D014709:Vero Cells; D014776:Virus Cultivation
D000534:Alum Compounds; C041524:aluminum sulfate