Isolation and characterization of a yeast strain carrying a mutation in the mitochondrial promoter for COX2.
Subunit II of cytochrome c oxidase is a mitochondrially encoded protein required for cellular respiration. A respiration-deficient yeast strain has been isolated and shown genetically to carry a mutation in the subunit II structural gene COX2. The respiration-deficient strain produces no subunit II polypeptide and is missing two major transcripts of the subunit II gene. The mutation, first mapped by rho- recombinational rescue to the 5' end of the gene, has been localized precisely, by DNA sequencing, 58 nucleotides upstream of the COX2 ATG initiation codon. The mutant strain carries a single nucleotide change (A to T) relative to the wild type sequence. This mutation occurs in a sequence with substantial homology to a previously identified yeast mitochondrial promoter consensus sequence. These results provide the first in vivo evidence for the importance of the consensus sequence for promoter function.
Cameron
V L
VL
Department of Biology, Ithaca College, New York 14850.
Fox
T D
TD
Poyton
R O
RO
D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D005796:Genes; D005800:Genes, Fungal; D005838:Genotype; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009154:Mutation; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D013045:Species Specificity
D004272:DNA, Mitochondrial; D046911:Macromolecular Substances; D003576:Electron Transport Complex IV