Regional distribution of metabotropic glutamate response in the rat brain using Xenopus oocytes.
The regional distribution of metabotropic L-glutamate responses was investigated in Xenopus oocytes injected with poly(A)+-RNA from a rat brain which was separated into 3 parts: cerebrum, cerebellum and brainstem. Under voltage-clamp, oscillatory current responses were induced more in cerebellum or brainstem poly (A)+-RNA-injected oocytes, and less in cerebrum poly(A)+-RNA-injected oocytes. These results suggest that the metabotropic glutamate receptor is distributed mostly in cerebellum and brainstem.
Horikoshi
T
T
Department of Physiology, School of Dental Medicine, Tsurumi University, Yokohama, Japan.
Asanuma
A
A
Yanagisawa
K
K
Anzai
K
K
Goto
S
S
D000818:Animals; D001923:Brain Chemistry; D001933:Brain Stem / Q000032:analysis; D002531:Cerebellum / Q000032:analysis; D005971:Glutamates / Q000378:metabolism*; D008564:Membrane Potentials; D009865:Oocytes / Q000378:metabolism; D011061:Poly A; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter / Q000032:analysis* / Q000235:genetics; D014981:Xenopus
D005971:Glutamates; D012333:RNA, Messenger; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter; D011061:Poly A