[The content of tyrosine in rat luteal cell and the changes caused by hCG, cAMP and progesterone].
The ovaries taken from the immature female rats primed with PMSG-hCG were digested with collagenase-DNAase solution to obtain the luteal cell suspensions. Luteal cells were then incubated with different doses of hCG, cAMP or progesterone for 1.5 hours. The contents of tyrosine in luteal cell suspensions were determined by thin layer chromatogram using dual-wavelength chromatogram scanner. It was found that the content of tyrosine in rat luteal cell suspensions was 1.41 + 0.24 nmol/L/10(6) cells after one hour incubation. hCG, cAMP and progesterone all could significantly increase the content of tyrosine in the suspensions, suggesting the release of "endogenous tyrosine". The increase of tyrosine was not due to increased transformation of phenylalanine, the precursor of tyrosine, and increased protein synthesis, because both phenylalanine and cycloheximide failed to influence such increase.
Zhang
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Q
Wang
X N
XN
Gao
E W
EW
Cheng
C P
CP
D000818:Animals; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D003338:Corpus Luteum / Q000378:metabolism*; D000242:Cyclic AMP / Q000494:pharmacology*; D005260:Female; D008184:Luteal Cells / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D014443:Tyrosine / Q000032:analysis / Q000378:metabolism*
D006063:Chorionic Gonadotropin; D014443:Tyrosine; D011374:Progesterone; D000242:Cyclic AMP