title: Virulence determinants of Salmonella typhimurium from animal sources.
abstract: Two hundred seventy-eight strains of Salmonella typhimurium isolated from 1973 to 1981 from animal sources in New York State were studied for possible virulence determinants and for a serotype-specific plasmid possibly linked with virulence. Of the strains, 98% possessed type-1 fimbriae. All strains possessed flagella and were motile. One hundred twenty-three strains (44%) treated with mitomycin C tested positive for the cholera-Escherichia coli heat labile family of toxins by a kinetics-based ELISA; when treated with mitomycin C and extracted with polymyxin B, 249 (90%) were positive in the kinetics-based ELISA. All strains were negative in the Biken test. A smooth cell wall was found in 99% of the strains. Sixty-one percent (169) of the strains had a 62-Md plasmid. Seventy-six (27%) of the strains had detectable plasmids ranging in size from 1 to 124 Md.
authors.affiliation: Diagnostic Laboratory, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853.
authors.forename: P L
authors.initials: PL
authors.lastname: McDonough
authors.forename: R H
authors.initials: RH
authors.lastname: Jacobson
authors.forename: J F
authors.initials: JF
authors.lastname: Timoney
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000032:analysis; D002473:Cell Wall / Q000648:ultrastructure; D004731:Endotoxins; D004768:Enterotoxins / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D010861:Fimbriae, Bacterial / Q000648:ultrastructure; D009518:New York; D010957:Plasmids; D012481:Salmonella Infections, Animal / Q000382:microbiology; D012486:Salmonella typhimurium / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity* / Q000648:ultrastructure; D014774:Virulence
chemical_list: D001427:Bacterial Toxins; D004731:Endotoxins; D004768:Enterotoxins; C027952:salmonella toxin