Phosphorylation of large tumour antigen by cdc2 stimulates SV40 DNA replication.
Simian virus 40 large tumour antigen (T) is a replication origin binding protein required for viral DNA synthesis. Unphosphorylated T antigen is deficient in promoting DNA replication in vitro but can be activated by phosphorylation at residue threonine 124 by the cdc2 protein kinase. This observation demonstrates that T is regulated by phosphorylation and provides a model for cdc2 function in the control of DNA replication.
McVey
D
D
Cold Spring Harbor Laboratory, New York 11724.
Brizuela
L
L
Mohr
I
I
Marshak
D R
DR
Gluzman
Y
Y
Beach
D
D
D000952:Antigens, Polyomavirus Transforming / Q000235:genetics / Q000302:isolation & purification; D016203:CDC2 Protein Kinase; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D006367:HeLa Cells / Q000201:enzymology; D006801:Humans; D007700:Kinetics; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D013539:Simian virus 40 / Q000235:genetics* / Q000276:immunology
D000952:Antigens, Polyomavirus Transforming; D004268:DNA-Binding Proteins; D010750:Phosphoproteins; D011494:Protein Kinases; D016203:CDC2 Protein Kinase