title: 2-Aminoethylarsonic acid as an analogue of ethanolamine phosphate. Endowment of ethanolamine-phosphate cytidylyltransferase with CTP pyrophosphatase activity.
abstract: 2-Aminoethylarsonic acid was tested for its ability to act as a substrate for ethanolamine-phosphate cytidylytransferase as a cytidylyl acceptor in place of ethanolamine phosphate. The expected product, like all mixed anhydrides of arsonic acids, should hydrolyse spontaneously with regeneration of the substrate analogue and CMP formation; such CMP production was observed. The limiting velocity with aminoethylarsonic acid is about 90% that with ethanolamine phosphate, and the Michaelis constant is below 20 mM.
authors.affiliation: Department of Biochemistry, University of Cambridge, U.K.
authors.forename: E
authors.initials: E
authors.lastname: Visedo-Gonzalez
authors.forename: H B
authors.initials: HB
authors.lastname: Dixon
mesh_terms: D001152:Arsenicals; D003570:Cytidine Triphosphate / Q000378:metabolism; D004983:Ethanolamines; D009713:Nucleotidyltransferases / Q000378:metabolism*; D011755:Pyrophosphatases / Q000378:metabolism*; D012316:RNA Nucleotidyltransferases
chemical_list: D001152:Arsenicals; D004983:Ethanolamines; C060619:2-aminoethylarsonic acid; D003570:Cytidine Triphosphate; C005448:phosphorylethanolamine; D009713:Nucleotidyltransferases; D012316:RNA Nucleotidyltransferases; C020126:Ethanolamine-phosphate cytidylyltransferase; D011755:Pyrophosphatases