Induction of protooncogene fos by extracellular signals in primary glial cell cultures.
In the present study various extracellular factors, acting through different second messenger systems, were examined for their capacity to increase the level of c-fos mRNA in primary glial cell cultures. In particular EGF, 12-O-tetradecanoylphorbol 13-acetate, the beta-adrenergic agonist isoproterenol, and the glutamate agonists, ibotenic and quisqualic acid, were studied. All the extracellular stimuli tested induced a rapid and transient increase in c-fos mRNA level in glial cell cultures regardless of the signal transduction pathway and the final effect on cell proliferation.
Condorelli
D F
DF
Institute of Biochemistry, Faculty of Medicine, University of Catania, Italy.
Kaczmarek
L
L
Nicoletti
F
F
Arcidiacono
A
A
Dell'Albani
P
P
Ingrao
F
F
Magrì
G
G
Malaguarnera
L
L
Avola
R
R
Messina
A
A
D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D001921:Brain / Q000166:cytology / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D007051:Ibotenic Acid / Q000494:pharmacology; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D015290:Second Messenger Systems / Q000187:drug effects*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D004268:DNA-Binding Proteins; D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D007051:Ibotenic Acid; D004815:Epidermal Growth Factor; D000242:Cyclic AMP; D013755:Tetradecanoylphorbol Acetate