title: Interleukin 6 decreases cell-cell association and increases motility of ductal breast carcinoma cells.
abstract: Treatment of transformed breast duct epithelial cells with IL-6 produces a unique cellular phenotype characterized by diminished proliferation and increased motility. Human ductal carcinoma cells (T-47D and ZR-75-1 lines) are typically epithelioid in shape and form compact colonies in culture. Time-lapse cinemicrography shows that some untreated cells can transiently become fusiform or stellate in shape and separate from each other within a colony, but they usually rejoin their neighbors. While IL-6 suppresses the proliferation of these carcinoma cells, the IL-6-treated cells generally become stellate or fusiform and show increased motility. These changes persist as long as the cells are exposed to IL-6. This results in the dispersal of cells within colonies. The effects on cell growth, shape, and motility are reversible upon removal of IL-6. IL-6-treated T-47D cells display diminished adherens-type cell junctions, as indicated by markedly decreased vinculin-containing adhesions and intercellular desmosomal attachments. The effects on ZR-75-1 cell shape, colony number, and DNA synthesis are dependent on IL-6 concentration in the range from 0.15 to 15 ng/ml. Higher concentrations are required in T-47D cells for equivalent effects. Anti-IL-6 immune serum blocks IL-6 action. IL-6 represents a well-characterized molecule that regulates both the proliferation and junction-forming ability of breast ductal carcinoma cells.
authors.affiliation: Rockefeller University, New York, New York 10021.
authors.forename: I
authors.initials: I
authors.lastname: Tamm
authors.forename: I
authors.initials: I
authors.lastname: Cardinale
authors.forename: J
authors.initials: J
authors.lastname: Krueger
authors.forename: J S
authors.initials: JS
authors.lastname: Murphy
authors.forename: L T
authors.initials: LT
authors.lastname: May
authors.forename: P B
authors.initials: PB
authors.lastname: Sehgal
mesh_terms: D001943:Breast Neoplasms / Q000473:pathology*; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000473:pathology*; D002448:Cell Adhesion / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D004247:DNA / Q000096:biosynthesis; D003896:Desmosomes / Q000648:ultrastructure; D006801:Humans; D066298:In Vitro Techniques; D007365:Intercellular Junctions / Q000187:drug effects; D015850:Interleukin-6 / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D015850:Interleukin-6; D004247:DNA