title: ATP-dependent peptide release from mitochondria of reticulocytes.
abstract: ATP-dependent release of TCA-precipitable peptides from mitochondria-containing stroma (MCS) is described. The process is independent of ubiquitin, but is sensitive to hemin and to heat treatment. Neither chloramphenicol nor EGTA inhibit. 50% of the activity is dependent on charged tRNA. The peptides released from MCS possess a molecular mass of about 1-5 kDa and are degraded to TCA-soluble compounds by a cytosolic protease system (fraction II) without ubiquitin.
authors.affiliation: Institute of Biochemistry, Humboldt University Berlin, GDR.
authors.forename: W
authors.initials: W
authors.lastname: Dubiel
authors.forename: I
authors.initials: I
authors.lastname: Drung
authors.forename: M
authors.initials: M
authors.lastname: Müller
authors.forename: S M
authors.initials: SM
authors.lastname: Rapoport
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002850:Chromatography, Gel; D006868:Hydrolysis; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D011817:Rabbits; D012156:Reticulocytes / Q000187:drug effects / Q000378:metabolism*; D014452:Ubiquitins / Q000494:pharmacology
chemical_list: D010455:Peptides; D014452:Ubiquitins; D000255:Adenosine Triphosphate