Translation of poly(A)+ mRNA encoding CFU-S proliferation stimulator of human fetal liver origin in Xenopus laevis oocytes.
Using the sodium dodecyl sulfate/chloroform/phenol method, total RNA was extracted from human fetal liver tissue with a yield of 870-1060 micrograms/g of tissue; mRNA was obtained after chromatography on an oligo(dT)-cellulose column. Experimental studies demonstrated the translation of a poly(A)+ mRNA encoding proliferation stimulator of human fetal liver origin in Xenopus laevis oocytes.
Wei
Q S
QS
Department of Experimental Hematology, Beijing Institute of Radiation Medicine, China.
Wu
C T
CT
Cao
J R
JR
Zhang
X Z
XZ
D000818:Animals; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000235:genetics*; D005260:Female; D006133:Growth Substances / Q000235:genetics*; D016298:Hematopoietic Cell Growth Factors; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D008099:Liver / Q000032:analysis / Q000196:embryology*; D008297:Male; D051379:Mice; D009865:Oocytes / Q000378:metabolism; D011061:Poly A / Q000235:genetics* / Q000302:isolation & purification; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification; D013154:Spleen / Q000166:cytology; D014982:Xenopus laevis
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D016298:Hematopoietic Cell Growth Factors; D012333:RNA, Messenger; D011061:Poly A