Oxygen gradients in CHO cells: measurement and characterization by electron spin resonance.
The concentration of oxygen within cells is important in many physiological and pathological processes, but such oxygen-dependent processes are generally studied as a function of the concentration of extracellular oxygen, due to a lack of suitable methods. Using a newly developed technique based on ESR spectroscopy, we show that respiration stimulation of a cell suspension can result in a significant difference between average intracellular and extracellular concentrations of oxygen. These results indicate that studies of oxygen-dependent phenomena in cells may require measurement of intracellular oxygen concentrations and imply that there are mechanisms in cells that restrict the free diffusion of oxygen.
Glockner
J F
JF
University of Illinois, Department of Medicine, Urbana 61801.
Swartz
H M
HM
Pals
M A
MA
D000818:Animals; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D002460:Cell Line; D006224:Cricetinae; D003593:Cytoplasm / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D005110:Extracellular Space / Q000378:metabolism; D010100:Oxygen / Q000502:physiology*; D010101:Oxygen Consumption / Q000187:drug effects
D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D010100:Oxygen