Behavior of the copper band of cytochrome c oxidase in rat brain during FC-43-for-blood substitution.
FC-43-for-blood substitution experiments were conducted in anesthetized rats to evaluate NIR spectroscopic responses by living brain to exchange transfusion at very low hematocrits. The NIR responses to the exchange process also assess the ability of multiwavelength algorithms to measure independent changes in the relative amounts of Hb, tHbO2 and oxidized cytochrome a,a3 in the tissue. These data indicate that FC-43 circulation at high FIO2 (1.00) delivers sufficient O2 to the rat brain to maintain the oxidation state of the mitochondrial oxidase near pre-exchange values. The results also confirm the ability of four wavelength algorithms to distinguish changes in the oxidized copper band of cytochrome a,a3 from changes in absorption by Hb and HbO2.
Piantadosi
C A
CA
Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710.
D000818:Animals; D001802:Blood Substitutes; D001921:Brain / Q000201:enzymology*; D002560:Cerebrovascular Circulation; D003300:Copper / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000378:metabolism*; D005466:Fluorocarbons; D007834:Lasers; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013055:Spectrophotometry, Infrared / Q000379:methods
D001802:Blood Substitutes; D005466:Fluorocarbons; C008807:perfluorotributylamine; D003300:Copper; D003576:Electron Transport Complex IV