title: Identification and characteristics of a novel mitochondrial 5'-nucleotidase in rat liver.
abstract: In rat liver mitochondria there exists an AMP-dephosphorylating activity which converts external 5'-AMP to adenosine. It exhibits a pH optimum of 7.5 and a Km(AMP) of 0.085 mM. Furthermore, this activity is stimulated by magnesium (Km = 0.5 mM) and seems to be not affected by low concentrations of ATP or ADP. From the characteristics of the enzyme the existence of a 5'-nucleotidase in rat liver mitochondria which is localized on the outer surface of the inner mitochondrial membrane was concluded. The enzyme may be important for the production of cellular adenosine.
authors.affiliation: Abteilung für experimentelle Organtransplantation, Leninallee, Berlin, DDR.
authors.forename: W
authors.initials: W
authors.lastname: Henke
authors.forename: M
authors.initials: M
authors.lastname: Lang
authors.forename: W
authors.initials: W
authors.lastname: Dubiel
authors.forename: H G
authors.initials: HG
authors.lastname: Holzhütter
authors.forename: G
authors.initials: G
authors.lastname: Gerber
mesh_terms: D015720:5'-Nucleotidase; D000249:Adenosine Monophosphate / Q000378:metabolism; D000818:Animals; D004789:Enzyme Activation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007425:Intracellular Membranes / Q000201:enzymology; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology*; D009708:Nucleotidases / Q000302:isolation & purification* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000249:Adenosine Monophosphate; D009708:Nucleotidases; D015720:5'-Nucleotidase; D008274:Magnesium