Bone marrow-derived stromal cell line expressing osteoblastic phenotype in vitro and osteogenic capacity in vivo.
Marrow stroma has been shown to have osteogenic potential. Here we report the characterization of a unique stromal cell line derived from mouse bone marrow (MBA-15), which expresses osteoblastic phenotype in vitro and forms bone in vivo. More than 70% of cells in culture were histochemically positive for alkaline phosphatase. The enzyme levels were enhanced threefold when cultures were treated with dexamethasone. Gel electrophoresis of [3H]-proline-labeled cultures showed that MBA-15 cells produced only type I collagen. These cells were responsive to PTH, as indicated by a 50-fold increase in intracellular cAMP. Prostaglandin E2, but not calcitonin, stimulated cAMP up to 70-fold. When cultures were grown to confluence and fed daily with ascorbic acid and beta-glycerophosphate, the cells formed a Von Kossa positive, thick extracellular matrix, shown to contain hydroxyapatite crystals. MBA-15 cells produced mineralized bone when implanted in diffusion chambers. These results indicate that the MBA-15 cell line possesses osteoblastic features in vitro and osteogenic capacity in vivo.
Benayahu
D
D
Department of Histology and Cell Biology, Sackler Faculty of Medicine, Tel Aviv University, Israel.
Kletter
Y
Y
Zipori
D
D
Wientroub
S
S
D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001853:Bone Marrow / Q000502:physiology*; D001854:Bone Marrow Cells; D002460:Cell Line; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D000242:Cyclic AMP / Q000032:analysis; D003907:Dexamethasone / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D051379:Mice; D010006:Osteoblasts / Q000378:metabolism / Q000502:physiology*; D010012:Osteogenesis; D010641:Phenotype; D011458:Prostaglandins E / Q000378:metabolism
D011458:Prostaglandins E; D003907:Dexamethasone; D003094:Collagen; D000242:Cyclic AMP; D000469:Alkaline Phosphatase