Characterization of a nalidixic-acid-resistant mutant of Escherichia coli as a strict aerobe.
An Escherichia coli mutant, C18, which plates at an efficiency of 5.0 x 10(-4) under anaerobic condition, was isolated among spontaneous nalidixic-acid-resistant mutants. This strict aerobic mutation was mapped by P1 cotransduction with a gyrA linked transposon Tn10 and found to be at the gyrA gene. A low degree of superhelicity of pBR322 DNA isolated from C18 was demonstrated by agarose gel electrophoresis with various concentrations of ethidium bromide. The superhelical density of pBR322 isolated from C18 was 80% of the value of pBR322 isolated from wild-type bacteria cultured under aerobic condition, and 50% cultured under anaerobic condition. These results lead us to conclude that a certain mutation of the gyrA gene causes a decrease in DNA superhelicity and prevents anaerobic growth.
Yoshizawa
Y
Y
Department of Microbiology and Immunology, Hahnemann University School of Medicine, Philadelphia, PA 19102.
Yamamoto
N
N
D000332:Aerobiosis; D002874:Chromosome Mapping; D004250:DNA Topoisomerases, Type II / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism; D004278:DNA, Superhelical / Q000378:metabolism; D004352:Drug Resistance, Microbial / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D009154:Mutation; D009268:Nalidixic Acid / Q000494:pharmacology*; D010957:Plasmids; D014161:Transduction, Genetic
D004269:DNA, Bacterial; D004278:DNA, Superhelical; D009268:Nalidixic Acid; D004250:DNA Topoisomerases, Type II