The effects of experimental uremia in rats on duodenal VIP levels and the interaction of VIP with duodenal epithelial cells.
The effects of experimental uremia on the concentration of vasoactive intestinal peptide (VIP) in duodenum as well as on the interaction of this neuropeptide with the corresponding epithelial cells were studied in rats. Duodenal VIP concentration was significantly decreased in uremic rats as compared to control animals. The specific binding of VIP to duodenal epithelial cells increased in rats with uremia due to an increase in the number of VIP receptors rather than a change in the binding affinity or in the extent of VIP degradation. On the other hand, the efficacy but not the potency of VIP upon cyclic AMP generation varied in parallel to that observed at the receptor level.
Fernández-Moreno
M D
MD
Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá, Madrid, Spain.
Arilla
E
E
Prieto
J C
JC
D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D004386:Duodenum / Q000166:cytology* / Q000378:metabolism; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D008026:Ligation / Q000009:adverse effects; D008297:Male; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D014511:Uremia / Q000378:metabolism / Q000503:physiopathology*; D014513:Ureter / Q000601:surgery; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism* / Q000502:physiology
D014660:Vasoactive Intestinal Peptide; D000242:Cyclic AMP