Regulation of DNA supercoiling in Escherichia coli: genetic basis of a compensatory mutation in DNA gyrase.
Bacterial DNA supercoiling is controlled by balancing the supercoiling activity of DNA gyrase and the relaxing activity of DNA topoisomerase I. We have characterized the gyrB gene from a top A deletion mutant of Escherichia coli (DM800) that has a compensatory mutation in gyrB, lowering the activity of gyrase 10-fold, and thereby redressing the intracellular level of supercoiling. The mutant gene differs from the wild type in carrying three rather than two direct tandem repeats of a 6 bp sequence encoding Ala-Arg. We suggest this novel mutation affects domain spacing and was generated by an unequal crossing over event, possibly involving gyrase.
McEachern
F
F
Department of Cellular and Molecular Sciences, St George's Hospital Medical School, University of London, England.
Fisher
L M
LM
D001483:Base Sequence; D004250:DNA Topoisomerases, Type II / Q000235:genetics*; D004278:DNA, Superhelical / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000648:ultrastructure; D005798:Genes, Bacterial; D009154:Mutation; D015183:Restriction Mapping; D013329:Structure-Activity Relationship
D004278:DNA, Superhelical; D004250:DNA Topoisomerases, Type II