title: Effects of infection by HIV-1, cytomegalovirus, and human measles virus on cultured human thymic epithelial cells.
abstract: A tissue culture system for the growth of human fetal and infantile thymic epithelial (TE) cells has been established and characterized. We have investigated the effects of infection of these cells by human cytomegalovirus (CMV), measles virus, and human immunodeficiency virus type-1 (HIV-1). In the case of CMV, morphological changes were apparent by 2-4 days after viral inoculation of infantile TE cells. CMV-related antigens were detected by immunofluorescence after 12 days, and progeny infectious CMV was recovered from culture media after 18 days. Following infection by measles virus, distinctive, multinucleated giant TE cells appeared in both cultures of fetal and infantile TE cells. Measles virus-inoculated TE cells displayed an altered phenotype, as revealed by reaction with monoclonal antibodies with specificity for a variety of TE markers. Finally, infection of TE cells by HIV-1 resulted in cellular disarrangement, increased numbers of Hassall's corpuscles, and multinucleated giant cells. An increase in the number of cells reactive with monoclonal antibodies, specific for Hassall's corpuscles, was observed in the case of cells infected by either measles virus or HIV-1. These findings suggest that a variety of different viruses can successfully infect thymic epithelial tissue. Because of the important role of the thymus in development of the immune system, it is reasonable to conclude that viral infection of thymic tissue might play an important role in virus-mediated suppression of immune responsiveness.
authors.affiliation: Montreal Children's Hospital Research Institute, Quebec, Canada.
authors.forename: K
authors.initials: K
authors.lastname: Numazaki
authors.forename: H
authors.initials: H
authors.lastname: Goldman
authors.forename: X Q
authors.initials: XQ
authors.lastname: Bai
authors.forename: I
authors.initials: I
authors.lastname: Wong
authors.forename: M A
authors.initials: MA
authors.lastname: Wainberg
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000276:immunology; D003586:Cytomegalovirus Infections / Q000276:immunology*; D004848:Epithelium / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007165:Immunosuppression Therapy; D008457:Measles / Q000276:immunology*; D008459:Measles virus / Q000276:immunology; D010641:Phenotype; D013950:Thymus Gland / Q000276:immunology / Q000382:microbiology* / Q000473:pathology
chemical_list: D000911:Antibodies, Monoclonal; D000956:Antigens, Viral