Type analysis of oligosaccharide chains on human and murine MHC class II alpha chains by the lectin-nitrocellulose sheet method.
1. The microheterogeneous alpha molecules of class II antigen, DR molecules obtained from human B cell line and I-A molecules from mouse B cell hybridoma cell line, were separated by 2-D PAGE, transferred onto NC sheets and N-linked oligosaccharide types were analyzed by staining with P.O./lectins. 2. This is the first report to show directly the type of oligosaccharide chain corresponding to each spot separated by 2-D PAGE. The glycosylation patterns of class II alpha chains in human and mouse were compared.
Katagiri
Y U
YU
Institute for Immunological Science, Hokkaido University, Sapporo, Japan.
Kijimoto-Ochiai
S
S
Hatae
T
T
Okuyama
H
H
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D003101:Collodion; D003208:Concanavalin A; D004591:Electrophoresis, Polyacrylamide Gel; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D037102:Lectins; D051379:Mice; D009439:Neuraminidase / Q000494:pharmacology; D009844:Oligosaccharides / Q000032:analysis*; D009195:Peroxidase; D037121:Plant Lectins
D000949:Histocompatibility Antigens Class II; D037102:Lectins; D009844:Oligosaccharides; D037121:Plant Lectins; C017707:Ricinus communis agglutinin-1; C033619:lentil lectin; D003208:Concanavalin A; D003101:Collodion; D009195:Peroxidase; D009439:Neuraminidase