Biochemical characterization of a digitalis-like substance in human urine, partially purified by high pressure liquid chromatography.
We have partially purified a digitalis-like extract from human urine by means of high pressure liquid chromatography (HPLC). Four fractions, giving positive activity for the cross-reaction in the digoxin radioimmunoassay, Na-K-ATPase inhibition or competition with ouabain binding to its receptor, were detected on HPLC. Among these fractions, the first (F-1) was shown to contain the principle fraction, sharing all the activities, and to exhibit quite similar characteristics to ouabain in the modes of Na-K-ATPase inhibition, ouabain displacement activity and positive inotropic action.
Takeda
R
R
Second Department of Internal Medicine, School of Medicine, Kanazawa University, Japan.
Morise
T
T
Ikeda
M
M
Okamoto
S
S
D000818:Animals; D001667:Binding, Competitive / Q000187:drug effects; D001798:Blood Proteins / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology / Q000652:urine*; D002298:Cardenolides; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003429:Cross Reactions; D004077:Digoxin / Q000276:immunology; D005260:Female; D006801:Humans; D008297:Male; D009200:Myocardial Contraction / Q000187:drug effects; D010042:Ouabain / Q000378:metabolism; D011863:Radioimmunoassay; D051381:Rats; D012503:Saponins; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000302:isolation & purification / Q000494:pharmacology / Q000652:urine; D013268:Stimulation, Chemical
D001798:Blood Proteins; D002298:Cardenolides; D012503:Saponins; C034504:digoxin-like factors; D010042:Ouabain; D004077:Digoxin; D000254:Sodium-Potassium-Exchanging ATPase