[Design of a nutrient medium for bacteria of the genus Haemophilus--producers of restriction endonucleases].
A culture medium for the cultivation of hemophilic bacteria, containing acidic casein hydrolysate, aminopeptide and fodder yeast extract, has been proposed. The growth-stimulating properties of this medium have been studied on 5 strains producing restrictases differing in their specificity. In growing these producer strains in a model ANKUM-2 fermenter with the supply of carbohydrate substrates (glucose, sucrose, glycerin) the yield of biomass, considered to be high for hemophilic bacteria (10-14 g of humid substance from 1 liter of the medium), has been achieved. As shown on H. influenzae Rc B-2297 used as an example, an increase in the yield of microbial biomass leads to a decrease in restrictase specific activity.
Poliachenko
V M
VM
Mel'nikova
V A
VA
Gruber
I M
IM
Smirnova
G A
GA
Raskin
B M
BM
D001431:Bacteriological Techniques / Q000295:instrumentation; D003470:Culture Media / Q000378:metabolism*; D004262:DNA Restriction Enzymes / Q000096:biosynthesis*; D005285:Fermentation; D006190:Haemophilus / Q000201:enzymology / Q000254:growth & development*; D006193:Haemophilus influenzae / Q000201:enzymology / Q000254:growth & development; D008970:Molecular Weight
D003470:Culture Media; D004262:DNA Restriction Enzymes
Konstruirovanie pitatel'noĭ sredy dlia bakteriĭ roda Haemophilus--produtsentov restriktiruiushchikh éndonukleaz.