title: Experimental infection of sheep with bovine leukemia virus: infectivity of blood, nasal and saliva secretions.
abstract: This study was designed to determine the relative infectivity of lymphocytes and secretions from BLV-infected cattle with and without persistent lymphocytosis (BLV+PL+ and BLV+PL-). Ninety-seven sheep of mixed sex and age were assembled into 21 experimental groups. The recipient sheep were inoculated intravenously with serial dilutions of whole blood, saliva or nasal secretions from BLV+PL+ and BLV+PL- donor cows. Between 200 to 20,000 cells from single and mixed BLV+PL+ or single and mixed BLV+PL- donor cattle were used for inoculation. A very small number of BLV-infected lymphocytes (200 cells) was sufficient to induce BLV infection in sheep inoculated with diluted whole blood from BLV+PL+ cattle. The inoculation of whole blood (containing up to 20,000 lymphocyte cells) from BLV+PL- cattle did not induce BLV infection in recipient sheep. Saliva and nasal secretions also failed to bring about BLV transmission.
authors.forename: M H
authors.initials: MH
authors.lastname: Gatei
authors.forename: M W
authors.initials: MW
authors.lastname: McLennan
authors.forename: M F
authors.initials: MF
authors.lastname: Lavin
authors.forename: R C
authors.initials: RC
authors.lastname: Daniel
mesh_terms: D000818:Animals; D001769:Blood / Q000382:microbiology; D002417:Cattle; D002418:Cattle Diseases / Q000635:transmission*; D005260:Female; D007938:Leukemia / Q000209:etiology / Q000662:veterinary*; D001909:Leukemia Virus, Bovine; D008297:Male; D009296:Nasal Cavity / Q000382:microbiology; D012463:Saliva / Q000382:microbiology; D012756:Sheep; D012757:Sheep Diseases / Q000209:etiology*