Protective effect of colchiceine against acute liver damage.
Pretreatment of rats with colchiceine (10 micrograms/day/rat) for seven days protected against CCl4-induced liver damage. CCl4 intoxication was demonstrated histologically and by increased serum activities of alanine amino transferase (ALT), alkaline phosphatase (Alk. Phosph.) gamma glutamyl transpeptidase (GGTP), bilirubins and decreased activity of glucose-6-phosphatase (G-6Pase). Furthermore, an increase in liver lipid peroxidation and a decrease in plasma membrane GGTP and Alk. Phosph. activities were found. Colchiceine increased 1.5-fold the LD50 of CCl4 and prevented the release of intracellular enzymes as well as the decrease in GGTP and Alk. Phosph. activities in plasma membranes. It also completely prevented the lipid peroxidation induced by CCl4 and limited the extent of the histological changes.
Mourelle
M
M
Departamento de Farmacología y Toxicología, Centro de Investigación y de Estudios Avanzados del I.P.N., México.
Fraginals
R
R
Rodríguez
L
L
Favari
L
L
Pérez-Alvarez
V
V
D000410:Alanine Transaminase / Q000097:blood / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000097:blood / Q000378:metabolism; D000818:Animals; D001663:Bilirubin / Q000097:blood; D002251:Carbon Tetrachloride; D002462:Cell Membrane / Q000201:enzymology; D003078:Colchicine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D005952:Glucose-6-Phosphatase / Q000097:blood / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008297:Male; D008745:Methylation; D051381:Rats; D011919:Rats, Inbred Strains; D005723:gamma-Glutamyltransferase / Q000097:blood / Q000378:metabolism
D002251:Carbon Tetrachloride; D005723:gamma-Glutamyltransferase; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D005952:Glucose-6-Phosphatase; C012852:colchiceine; D001663:Bilirubin; D003078:Colchicine