Long-range restriction enzyme maps of DNF15S2, D3S2, and c-raf1 loci on the short arm of human chromosome 3.
Physical maps have been constructed around loci DNF15S2, D3S2, and c-raf1 on the short arm of human chromosome 3 using pulsed field gradient gel electrophoresis. The normal restriction pattern has not been altered by a t(3;8)(p14.2;q24.1) characteristic for a hereditary form of renal cell carcinoma, indicating that the breakpoint itself is not included in any of the mapped areas. We have found a CpG island within the DNF15S2 locus, suggesting the presence of a functional gene in the region.
Boldog
F
F
Department of Tumor Biology, Karolinska Institute, Stockholm, Sweden.
Erlandsson
R
R
Klein
G
G
Sumegi
J
J
D015139:Blotting, Southern; D002292:Carcinoma, Renal Cell / Q000235:genetics; D002460:Cell Line; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics; D008214:Lymphocytes; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping