title: Maintaining mosquito cell lines at high temperatures: effects on the replication of flaviviruses.
abstract: The upper thermal limit for maintenance of eleven mosquito cell lines was studied. Although most cell lines could be grown at 32 degrees C to 34 degrees C, Anopheles stephensi cell line could be maintained at 37 degrees C. At higher temperatures initial growth rate was higher, but yield of cells after about a week of incubation was lower than at the standard temperature (28 degrees C). Replication of several flaviviruses in Aedes albopictus cell cultures adapted to 34.5 degrees C was faster, and viral titers were higher than at 28 degrees C.
authors.affiliation: Division of Vector-Borne Viral Diseases, Center for Infections Diseases, U.S. Department of Health and Human Services, San Juan, Puerto Rico 00936.
authors.forename: G
authors.initials: G
authors.lastname: Kuno
authors.forename: A
authors.initials: A
authors.lastname: Oliver
mesh_terms: D000818:Animals; D002455:Cell Division; D002460:Cell Line / Q000166:cytology* / Q000382:microbiology; D009033:Culicidae / Q000166:cytology* / Q000382:microbiology; D005416:Flavivirus / Q000502:physiology*; D013696:Temperature; D014779:Virus Replication