Identification of a multispecific lipoprotein receptor in adult Schistosoma japonicum by ligand blotting analyses.
A 43-kDa putative lipoprotein receptor (Sj43) of adult Schistosoma japonicum worms has been identified using ligand blotting techniques. Single and two dimensional electrophoretic analyses showed that Sj43 consisted of a single acidic polypeptide with multiple lipoprotein specificity. The molecule bound 125I-labelled low-density (apo-B), very low-density or high-density (apo-A and/or apo-C) lipoproteins from different mammalian hosts that are permissive to S. japonicum infection, but did not bind mouse apo-A containing lipoprotein. The binding of 125I-labelled lipoprotein to Sj43 could be inhibited by unlabelled human LDL, EDTA or Suramin, or by chemical modification of lipoprotein lysine or arginine residues. Sj43 was localised at the parasite's tegument and gut lining.
Rogers
M V
MV
Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia.
Henkle
K J
KJ
Fidge
N H
NH
Mitchell
G F
GF
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D007457:Iodine Radioisotopes; D008024:Ligands; D008074:Lipoproteins / Q000302:isolation & purification / Q000378:metabolism*; D008075:Lipoproteins, HDL / Q000302:isolation & purification / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000302:isolation & purification / Q000378:metabolism; D051379:Mice; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000378:metabolism; D018110:Receptors, Lipoprotein; D012549:Schistosoma japonicum / Q000032:analysis* / Q000378:metabolism; D013045:Species Specificity
D007457:Iodine Radioisotopes; D008024:Ligands; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D011956:Receptors, Cell Surface; D018110:Receptors, Lipoprotein