[An alpha-satellite DNA sequence, alpha-RI-6, specific for human chromosomes 13 and 21, detected using the RFLP technic with digoxigenin labelled probes].
The authors compared two at present most widely used techniques for labelling DNA probes: a) radioactive labelling by means of the radioisotope 32P; non-radioactive labelling using the hapten digoxigenin for the visualization of the hybridization process on nylon membranes. Then sensitivity of the technique of non-radioactive labelling of heterochromatin probes was equivalent to the radioactive method.
Mazura
I
I
Brdicka
R
R
Srám
R J
RJ
Jurov
J
J
Kapras
J
J
D015139:Blotting, Southern; D002882:Chromosomes, Human, Pair 13; D002891:Chromosomes, Human, Pair 21; D015342:DNA Probes; D004276:DNA, Satellite / Q000032:analysis / Q000235:genetics*; D004076:Digoxigenin; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid
D015342:DNA Probes; D004276:DNA, Satellite; D004076:Digoxigenin
Alfa-satelitní DNA-sekvence, alfa-RI-6, specificka pro lidské chromosomy 13 a 21, detekovaná pomocí RFLP-techniky s digoxigeninovým znacením sondy.