title: Linkage analysis of the mutation locus in the eye lens obsolescence (Elo) mouse.
abstract: To map precisely the mutation locus of eye lens obsolescence (Elo) on mouse chromosome 1, subsequent linkage analysis was achieved using backcross mating between 129/SvSl-Elo (Elo/+) and 129/SvSl (+/+). Mouse genomic DNAs from 17 strains including the Elo mutant mouse were first digested with several restriction enzymes and analyzed by hybridization using gamma 2- and gamma 4-crystallin cDNAs as probes. Restriction endonuclease DraI showed distinct RFLP patterns in both cases. When gamma 2-crystallin cDNA was used as the probe, two strong bands were observed at 4.0 and 2.4 kb in the majority of strains, but the former fragment shifted to the 3.4-kb position in 129/SvSl-Elo (Elo/Elo) and CFO. The polymorphism between 4.0- and 3.4-kb fragments corresponded to the gamma 1-crystallin locus (Cryg-1), and that of the 2.4-kb one, to the gamma 2-crystallin locus (Cryg-2). Mouse DNAs were also analyzed by hybridization using gamma 4-crystallin cDNA (Cryg-4). In this case, 3.4- and 3.0-kb fragments were observed in Elo and wild-type mice, respectively. The backcross offsprings were analyzed with respect to Elo, Idh-1, Cryg-1, and Cryg-4 loci. Among 223 mice analyzed, recombination between Elo and Idh-1 loci was observed in three offsprings; and that between Cryg-1 or Cryg-4 and Idh-1 loci, in one offspring. No recombination occurred between Cryg-1 and Cryg-4 alleles.
authors.affiliation: Department of Biochemistry, Institute for Developmental Research, Aichi Prefecture Colony, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Masaki
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
mesh_terms: D000483:Alleles; D000818:Animals; D002874:Chromosome Mapping; D008040:Genetic Linkage; D005838:Genotype; D007908:Lens, Crystalline / Q000002:abnormalities*; D051379:Mice; D008817:Mice, Mutant Strains / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length