Computer analysis of 1,25-dihydroxyvitamin D3-receptor regulated promoters: identification of a candidate D3-response element.
The receptor for the steroid hormone 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] belongs to a superfamily of steroid and triidothyronine (T3) receptors. As yet no 1,25(OH)2D3 response element (DRE) has been identified. Since the T3 and 1,25(OH)2D3 receptors are structurally homologous, we have used the nucleotide sequence of the T3 response element to carry out a computer search of the promoter region of several 1,25(OH)2D3 regulated genes. We report here one candidate DRE from the chick calbindin-D28K gene (AGCCCAATGGCTGAACA) and two candidate DRE's from the rat osteocalcin gene (TCCCCACTGGATGAGCG and CCTGCACTGGGTGAATGA).
Minghetti
P P
PP
Department of Biochemistry, University of California, Riverside 92521.
Gibbs
P E
PE
Norman
A W
AW
D000818:Animals; D001483:Base Sequence; D064092:Calbindin 1; D064026:Calbindins; D002117:Calcitriol / Q000494:pharmacology*; D002135:Calcium-Binding Proteins / Q000235:genetics*; D002645:Chickens; D004247:DNA / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D008969:Molecular Sequence Data; D015675:Osteocalcin; D011401:Promoter Regions, Genetic; D051381:Rats; D018167:Receptors, Calcitriol; D011987:Receptors, Steroid / Q000502:physiology*; D011988:Receptors, Thyroid Hormone / Q000502:physiology; D012045:Regulatory Sequences, Nucleic Acid; D064030:S100 Calcium Binding Protein G / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
C576498:CALB1 protein, human; C576500:Calb1 protein, rat; D064092:Calbindin 1; D064026:Calbindins; D002135:Calcium-Binding Proteins; D018167:Receptors, Calcitriol; D011987:Receptors, Steroid; D011988:Receptors, Thyroid Hormone; D064030:S100 Calcium Binding Protein G; D015675:Osteocalcin; D004247:DNA; D002117:Calcitriol