title: Two genes encode 7SL RNAs in the yeast Yarrowia lipolytica.
abstract: We have identified an abundant cytoplasmic 7S RNA in crude extracts of the yeast Yarrowia lipolytica. A cDNA probe was prepared from this RNA and used to screen a genomic library. The DNA sequence of a positive clone was determined and the end positions of the 7S RNA gene established by comparison with the sequence of the extremities of 7S RNA. This gene, designated SCR2, encodes a 270-nucleotide RNA that can be folded into a secondary structure similar to that of 7SL RNAs. This RNA is 94.4% homologous to a previously identified 7S RNA from this yeast, but is encoded by a separate gene with highly divergent flanking sequences.
authors.affiliation: Laboratoire de génétique des microorganismes, Institut National A gronomique, Thiverval Grignon, France.
authors.forename: F
authors.initials: F
authors.lastname: He
authors.forename: J M
authors.initials: JM
authors.lastname: Beckerich
authors.forename: V
authors.initials: V
authors.lastname: Ribes
authors.forename: D
authors.initials: D
authors.lastname: Tollervey
authors.forename: C M
authors.initials: CM
authors.lastname: Gaillardin
mesh_terms: D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012331:RNA, Fungal / Q000235:genetics*; D015183:Restriction Mapping; D012261:Ribonucleoproteins / Q000235:genetics*; D004718:Saccharomycetales / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D018271:Signal Recognition Particle
chemical_list: D004271:DNA, Fungal; D012331:RNA, Fungal; D012261:Ribonucleoproteins; D018271:Signal Recognition Particle; D004247:DNA