title: In vitro activity of combinations of beta-lactam antibiotics with beta-lactamase inhibitors against cephalosporinase-producing bacteria.
abstract: Combinations of different beta-lactam antibiotics, including cefotaxime, with three beta-lactamase inhibitors were tested against cephalosporinase producing bacterial strains. The most significant antagonism was obtained with a combination of clavulanic acid and cefotaxime, while almost no antagonism was observed with sulbactam and tazobactam. In strains belonging to five different species there was a correlation between the levels of cephalosporinase produced after exposure to different concentrations of inhibitors and the MICs of cefotaxime combined with the same concentrations of inhibitors. It is concluded that there is little likelihood of antagonism between beta-lactam antibiotics and sulbactam or tazobactam.
authors.affiliation: Laboratoire de Microbiologie Médicale, Hôpital Saint-Joseph, Paris, France.
authors.forename: M D
authors.initials: MD
authors.lastname: Kitzis
authors.forename: B
authors.initials: B
authors.lastname: Ferré
authors.forename: A
authors.initials: A
authors.lastname: Coutrot
authors.forename: J F
authors.initials: JF
authors.lastname: Acar
authors.forename: L
authors.initials: L
authors.lastname: Gutmann
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002439:Cefotaxime / Q000494:pharmacology; D002510:Cephalosporinase / Q000096:biosynthesis*; D019818:Clavulanic Acid; D002969:Clavulanic Acids / Q000494:pharmacology; D004338:Drug Combinations; D004347:Drug Interactions; D004352:Drug Resistance, Microbial; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000201:enzymology; D004790:Enzyme Induction; D006801:Humans; D007525:Isoelectric Focusing; D008826:Microbial Sensitivity Tests; D010397:Penicillanic Acid / Q000494:pharmacology; D010878:Piperacillin / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000201:enzymology; D013407:Sulbactam / Q000494:pharmacology; D000078142:Tazobactam; D013982:Ticarcillin / Q000494:pharmacology; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases / Q000096:biosynthesis*
chemical_list: D000900:Anti-Bacterial Agents; D002969:Clavulanic Acids; D004338:Drug Combinations; D065093:beta-Lactamase Inhibitors; D019818:Clavulanic Acid; D010397:Penicillanic Acid; D002510:Cephalosporinase; D001618:beta-Lactamases; D013982:Ticarcillin; D002439:Cefotaxime; D013407:Sulbactam; D000078142:Tazobactam; D010878:Piperacillin