Preventive effects of pyrroloquinoline quinone on formation of cataract and decline of lenticular and hepatic glutathione of developing chick embryo after glucocorticoid treatment.
When 0.25 mumol of hydrocortisone succinate sodium (HC) was administered to 15-day-old fertile eggs, almost all lenses of chick embryos treated with HC for 48 hr were classified as cataract stage IV-V (95%). A triple application of potassium pyrroloquinoline quinone (PQQ) (1.25 mumol/egg) at 3, 10 and 20 hr after HC treatment showed a preventive effect against the HC-induced cataract formation (I:45%, II:25%, III: 30%). PQQ also prevented the decline of GSH in the lens caused by HC. The decline of GSH in liver 24 hr after HC administration was prevented by PQQ. These data indicate that PQQ can modify HC-induced effects and that the preventive effect of PQQ against HC-induced decline of hepatic GSH seemed to influence HC-induced events in lens.
Nishigori
H
H
Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
Yasunaga
M
M
Mizumura
M
M
Lee
J W
JW
Iwatsuru
M
M
D000818:Animals; D002386:Cataract / Q000139:chemically induced / Q000517:prevention & control*; D002642:Chick Embryo; D003067:Coenzymes / Q000627:therapeutic use*; D005938:Glucocorticoids / Q000633:toxicity*; D005978:Glutathione / Q000378:metabolism*; D007908:Lens, Crystalline / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D045542:PQQ Cofactor; D015363:Quinolones / Q000627:therapeutic use*
D003067:Coenzymes; D005938:Glucocorticoids; D015363:Quinolones; D045542:PQQ Cofactor; D005978:Glutathione