Lack of aldosterone inhibition by atrial natriuretic factor in primary aldosteronism: in vitro studies.
Several studies demonstrated that aldosterone secretion, by bovine, rat and human glomerulosa cells, is inhibited in vitro by atrial natriuretic factor (ANF). This effect has also been investigated with conflicting results in cells taken from aldosterone-producing tumors. In the present study, atrial natriuretic factor has been tested on aldosteronoma cells obtained from 4 patients with primary aldosteronism. The cells were studied both with perfusion and incubation systems. Aldosterone secretion was stimulated by ACTH, angiotensin II and potassium with or without ANF 10 microM. In this study ANF lacked to inhibit either basal and stimulated aldosterone secretion, indicating some alterations of ANF-adrenal interaction in this syndrome.
Rocco
S
S
Istituto di Semeiotica Medica, Università di Padova, Italy.
Opocher
G
G
D'Agostino
D
D
Leone
L
L
Mantero
F
F
D000236:Adenoma / Q000378:metabolism*; D000310:Adrenal Gland Neoplasms / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000450:Aldosterone / Q000378:metabolism*; D000804:Angiotensin II / Q000494:pharmacology; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D006801:Humans; D006929:Hyperaldosteronism / Q000378:metabolism*; D010477:Perfusion; D011188:Potassium / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000804:Angiotensin II; D000450:Aldosterone; D009320:Atrial Natriuretic Factor; D000324:Adrenocorticotropic Hormone; D011188:Potassium