The effects of 1,3-dinitrobenzene and mono-(2-ethylhexyl)phthalate on hormonally stimulated lactate and pyruvate production by rat Sertoli cell cultures.
Lactate and pyruvate production by rat Sertoli cell cultures was measured after treatment with two toxicants (1,3-dinitrobenzene, DNB; mono-(2-ethylhexyl)phthalate, MEHP) in the presence or absence of follicle-stimulating hormone (FSH) or dibutyryl-cAMP (dbcAMP). 1,3-DNB together with FSH or dbcAMP produced increases in media lactate and pyruvate concentrations significantly greater than those induced by individual treatments. MEHP in conjunction with either hormone also produced greater increases in lactate concentrations compared with separate additions. However, MEHP produced a significant diminution in the hormonal stimulation of pyruvate secretion. Thus, 1,3-DNB appears to act independently of these hormones in stimulating the secretion of both metabolic products, whereas MEHP interferes with the hormonal stimulation of pyruvate (but not lactate) production.
Williams
J
J
ICI Central Toxicology Laboratory, Alderley Park, Nr. Macclesfield, Cheshire, U.K.
Foster
P M
PM
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002478:Cells, Cultured; D004051:Diethylhexyl Phthalate / Q000031:analogs & derivatives / Q000633:toxicity*; D004136:Dinitrobenzenes / Q000633:toxicity*; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D007773:Lactates / Q000096:biosynthesis*; D008297:Male; D009578:Nitrobenzenes / Q000633:toxicity*; D010795:Phthalic Acids / Q000633:toxicity*; D011773:Pyruvates / Q000096:biosynthesis*; D019289:Pyruvic Acid; D051381:Rats; D011919:Rats, Inbred Strains; D012708:Sertoli Cells / Q000378:metabolism*
D004136:Dinitrobenzenes; D007773:Lactates; D009578:Nitrobenzenes; D010795:Phthalic Acids; D011773:Pyruvates; D003994:Bucladesine; D019289:Pyruvic Acid; D005640:Follicle Stimulating Hormone; D004051:Diethylhexyl Phthalate; C017906:3-dinitrobenzene; C016599:mono-(2-ethylhexyl)phthalate