title: S-phase evaluation with bromodeoxyuridine in lymphocytes from cattle infected with bovine leukemia virus (BLV).
abstract: Bromodeoxyuridine (BrdUrd), an analogue of thymidine, can be detected by means of monoclonal antibodies and utilized as a marker of the S-phase. In this paper a determination of the S-phase in BLV+ cattle with lymphocytosis has been performed by incorporating bromodeoxyuridine in the DNA. This evaluation was compared to the DNA content, demonstrating that i) bromodeoxyuridine incorporation is a reliable marker of S-phase in BLV+ cattle with lymphocytosis and ii) cytofluorimetry is the method of choice, together with immunocytochemistry, to demonstrate bromodeoxyuridine incorporation.
authors.forename: P L
authors.initials: PL
authors.lastname: Tazzari
authors.forename: M
authors.initials: M
authors.lastname: Cipone
authors.forename: C
authors.initials: C
authors.lastname: Tassi
authors.forename: A
authors.initials: A
authors.lastname: Bontadini
authors.forename: A M
authors.initials: AM
authors.lastname: Cremonini
authors.forename: M
authors.initials: M
authors.lastname: Gobbi
authors.forename: G
authors.initials: G
authors.lastname: Gentile
authors.forename: S
authors.initials: S
authors.lastname: Tura
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine; D002417:Cattle; D002418:Cattle Diseases / Q000473:pathology*; D002455:Cell Division; D005260:Female; D007150:Immunohistochemistry; D007399:Interphase; D007938:Leukemia / Q000473:pathology / Q000662:veterinary*; D001909:Leukemia Virus, Bovine; D008214:Lymphocytes / Q000473:pathology*
chemical_list: D001973:Bromodeoxyuridine