[Optimization of the determination in urine of alanine aminopeptidase, gamma-glutamyltransferase and N acetyl-beta-D-glucosaminidase].
The authors describe the optimization of determination of alanine aminopeptidase (AAP), gamma-glutamyltransferase (GGT) and N-acetyl-beta-D-glucosaminidase (NAG) in urine by multivariate analysis. The optimal conditions found are: for AAP at 30 degrees C TRIS HCl buffer 300 mmol/l pH 7.9, L-alanine-4-nitroanilide 5.8 mmol/l, for GGT at 30 degrees C buffer glycylglycine 150 mmol/l pH 8.0, gamma-L-glutamyl-3-carboxy-4-nitroanilide 9.0 mmol/l, for NAG at 37 degrees C citrate buffer 50 mmol/l pH 5.8, m cresolsulfonphtaleinyl-N-acetyl-beta-D-glucosaminide 5.5 mmol/l. These methods are easy to perform, apply to urine without pretreatment through Sephadex: therefore complete automatization is possible. The stability of enzymatic activities in urine is of ten days at +4 degrees C in the presence of sodium azide at neutral pH. Freezing resulted in a considerable loss of activity for AAP and GGT.
Flandrois
C
C
Laboratoire de Biochimie, Hôpital Debrousse, Lyon.
Lahet
C
C
Feldmann
D
D
Maire
I
I
Aymard
P
P
Mathieu
M
M
D000118:Acetylglucosaminidase / Q000378:metabolism / Q000652:urine*; D000626:Aminopeptidases / Q000378:metabolism / Q000652:urine*; D000704:Analysis of Variance; D018826:CD13 Antigens; D006596:Hexosaminidases / Q000652:urine*; D006801:Humans; D005723:gamma-Glutamyltransferase / Q000378:metabolism / Q000652:urine*
D005723:gamma-Glutamyltransferase; D006596:Hexosaminidases; D000118:Acetylglucosaminidase; D000626:Aminopeptidases; D018826:CD13 Antigens
Optimisation de la mesure dans l'urine de l'alanine aminopeptidase, de la gamma-glutamyltransférase et de la N-acétyl-bêta-D-glucosaminidase.