A microtiter plate assay for inorganic phosphate.
A microtiter assay for the detection of picomolar quantities of inorganic phosphate has been described. The assay, linear between 50 and 1000 pmol of inorganic phosphate, is simple and rapid, with results obtainable in several minutes. Results from 5'-nucleotidase and (Ca2+ + Mg2+)ATPase assays using this method were compared with conventional phosphate assays and showed a high degree of correlation. The high sensitivity of this assay and the small sample size needed allows its widespread use in biochemical studies involving the generation of inorganic phosphate.
Hoenig
M
M
Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia, Athens 30602.
Lee
R J
RJ
Ferguson
D C
DC
D015720:5'-Nucleotidase / Q000032:analysis*; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000032:analysis*; D000252:Calcium-Transporting ATPases / Q000032:analysis*; D002462:Cell Membrane / Q000201:enzymology; D004396:Coloring Agents; D007340:Insulinoma / Q000201:enzymology; D008832:Microchemistry; D010190:Pancreatic Neoplasms / Q000201:enzymology; D010710:Phosphates / Q000032:analysis*; D012394:Rosaniline Dyes; D013053:Spectrophotometry / Q000379:methods
D004396:Coloring Agents; D010710:Phosphates; D012394:Rosaniline Dyes; C005095:malachite green; D015720:5'-Nucleotidase; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases