title: Granulocyte-macrophage colony-stimulating factor (GM-CSF) and opsonization synergistically enhance leukotriene B4 (LTB4) synthesis induced by phagocytosis in human neutrophils.
abstract: Normal human blood neutrophils were studied for their capacity to synthesize leukotriene B4 (LTB4) and its omega-oxidized metabolites after phagocytosis of zymosan. Phagocytosis of serum-opsonized particles led to a higher release of LTs than did unopsonized zymosan. The most striking effect of phagocytosis was observed when neutrophils were primed with granulocyte-macrophage colony-stimulating factor (GM-CSF): opsonization and GM-CSF synergistically increased LTB4 synthesis by neutrophils.
authors.affiliation: Unité de Recherche Inflammation et Immunologie-Rhumatologie, Centre de Recherche du CHUL, Qué, Canada.
authors.forename: P E
authors.initials: PE
authors.lastname: Poubelle
authors.forename: S
authors.initials: S
authors.lastname: Bourgoin
authors.forename: P H
authors.initials: PH
authors.lastname: Naccache
authors.forename: P
authors.initials: P
authors.lastname: Borgeat
mesh_terms: D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003115:Colony-Stimulating Factors / Q000494:pharmacology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007975:Leukotriene B4 / Q000096:biosynthesis*; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D009895:Opsonin Proteins / Q000032:analysis; D010587:Phagocytosis / Q000187:drug effects; D015054:Zymosan / Q000494:pharmacology
chemical_list: D003115:Colony-Stimulating Factors; D006133:Growth Substances; D009895:Opsonin Proteins; D007975:Leukotriene B4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D015054:Zymosan; D001094:Arachidonate 5-Lipoxygenase