Effects of bradykinin on prostaglandin synthesis and cytosolic calcium in rabbit subcultured renal cortical smooth muscle cells.
Smooth muscle cells were cultured from an arteriole-rich fraction of the rabbit renal cortex and characterized by their ultrastructural and immunohistochemical features, their high content in creatine kinase (60-times that of the initial preparation) and their ability to synthesize renin. Cells, studied between passages 2 and 5, produced mainly PGE2 and, to a lesser extent, PGF2 alpha. Bradykinin (BK) (0.1 nM-1 microM) induced a concentration-dependent increase in PGE2 (28-40-times basal value at 1 microM after a 5 min incubation period) and stimulated also the free cytosolic calcium concentration [( Ca2+]i) with a 2-fold maximal rise to its basal value. Both effects, inhibited by the anti-B2 receptor [Thi5.8D-Phe7] BK, were not reproduced by DesArg9 BK. A decrease in the extracellular calcium concentration and incubation in the presence of a calcium-channel blocker (lanthanum chloride) inhibited the BK-dependent rise of [Ca2+]i but not that of PGE2. Preincubation with phorbol myristate acetate increased basal and BK-induced PGE2 synthesis but prevented the effect of BK on [Ca2+]i. These results demonstrate the ability of BK to increase [Ca2+]i and PGE2 production in cultured vascular cells from the rabbit renal cortex and suggest that kinins might act on the cortical microcirculation via their direct effects on arteriolar smooth muscle cells.
Dussaule
J C
JC
INSERM 64, Hôpital Tenon, Paris, France.
Béa
M L
ML
Baud
L
L
Ronco
P
P
Chansel
D
D
Helwig
J J
JJ
Ardaillou
R
R
D000818:Animals; D001011:Aorta / Q000378:metabolism; D001920:Bradykinin / Q000031:analogs & derivatives / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002478:Cells, Cultured; D003402:Creatine Kinase / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D015237:Dinoprost / Q000096:biosynthesis*; D015232:Dinoprostone / Q000096:biosynthesis*; D005455:Fluorescent Antibody Technique; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D007700:Kinetics; D007811:Lanthanum / Q000494:pharmacology; D008854:Microscopy, Electron; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D009131:Muscle, Smooth, Vascular / Q000378:metabolism; D011817:Rabbits; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D002121:Calcium Channel Blockers; C028521:lanthanum chloride; D007811:Lanthanum; D015237:Dinoprost; D005723:gamma-Glutamyltransferase; D003402:Creatine Kinase; D015232:Dinoprostone; D001920:Bradykinin; D002118:Calcium