CD8 suppressor cell activity and its effect on CD4 helper cell-dependent growth of SJL/J B-cell lymphomas.
CD8 cells, flow cytometrically sorted from the lymph nodes of tumor-bearing and normal SJL/J mice, suppressed in vitro proliferation of syngeneic CD4 cells in response to concanavalin A, two independent SJL/J lymphomas, and LPS-activated syngeneic B-cell blasts. The data confirm earlier reports that nonspecific suppressor cells are generated as a consequence of SJL/J lymphoma-stimulated T-cell proliferation. Earlier reports are extended, in that the suppressor cell is identified as expressing CD8, and the suppressor activity is shown to decrease the tumor-stimulated CD4 cell proliferation which is essential to growth of these CD4-dependent murine B-cell lymphomas. In three separate experiments, anti-CD8 treatment of mice, in which CD4 cells were made limiting by injection with anti-CD4, increased growth of transplantable SJL/J lymphomas with corresponding increases in numbers of CD4 cells. The data imply that, under certain conditions, CD8 suppressor cells measurably influence growth of SJL/J lymphomas by regulating the tumor-stimulated CD4 cell proliferation essential to maximum growth of SJL/J lymphomas.
Thrush
G R
GR
Wayne State University School of Medicine, Department of Immunology and Microbiology, Detroit, Michigan 48201.
Butch
A W
AW
Lerman
S P
SP
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D001402:B-Lymphocytes / Q000166:cytology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D016827:CD8 Antigens; D002455:Cell Division; D003208:Concanavalin A / Q000494:pharmacology; D007158:Immunologic Techniques; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D008228:Lymphoma, Non-Hodgkin / Q000473:pathology*; D051379:Mice; D008815:Mice, Inbred Strains; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D000945:Antigens, Differentiation, T-Lymphocyte; D016827:CD8 Antigens; D008070:Lipopolysaccharides; D003208:Concanavalin A