Establishment and characterization of an Epstein-Barr virus transformed cell line with strong phagocytic activity.
An immunoglobulin M (IgM)-positive cell line, Ms 28, apparently spontaneously transformed by Epstein-Barr virus (EBV) was established from peripheral blood cells of a patient with immature myeloblastic leukemia. It has been characterized according to phenotype, cytochemistry, and membrane antigen pattern. The cell line expresses lymphoid markers like CD 19, CD 22, and CD 30 and synthesizes and secretes IgM. Monocyte markers CD 11c, CD 14, and CD 15 are absent. Neither interleukin-1 (IL-1), nor tumor necrosis factor (TNF-alpha) are produced. But Ms 28 cells show strong phagocytic activity and engulf Latex particles and sheep RBCs (SRBCs) that need not to be opsonized. The phagocytic activity can be inhibited by chloroquine. Both phagocytosis and EBV nuclear-antigen (EBNA) expression can be observed in one and the same cell. Ms 28 cells might be useful to study immunologic activities like antigen processing and presentation.
von den Driesch
P
P
Institut für Immunologie, Universität Münster, West Germany.
Bhardwaj
R
R
Flad
H D
HD
Neugebauer
D C
DC
Pielken
H J
HJ
Urbanitz
D
D
Kölsch
E
E
D000943:Antigens, Differentiation / Q000032:analysis; D002454:Cell Differentiation; D002455:Cell Division; D002462:Cell Membrane / Q000276:immunology; D002472:Cell Transformation, Viral; D004854:Herpesvirus 4, Human; D006801:Humans; D007075:Immunoglobulin M / Q000096:biosynthesis; D007375:Interleukin-1 / Q000096:biosynthesis; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D008854:Microscopy, Electron; D010586:Phagocytes / Q000145:classification / Q000166:cytology*; D010587:Phagocytosis; D014407:Tumor Cells, Cultured / Q000145:classification / Q000503:physiopathology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D000943:Antigens, Differentiation; D007075:Immunoglobulin M; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha