Relationship between cell cycle stage in the fertilized egg of mice and repair capacity for X-ray-induced damage in the sperm.
The potentiation effects of 3-aminobenzamide, caffeine, hydroxyurea and arabinofuranosyl cytosine on the yield of X-ray-induced chromosome aberrations of mouse sperm were examined at the first-cleavage metaphase, to clarify a correlation between chromosome aberrations and cell cycle dependency of repair capacity of the fertilized egg. The result provided evidence that there are two major types of DNA damage in X-irradiated sperm: (1) short-lived DNA lesions; the lesions are subject to repair inhibition by agents added in G1 and are converted into chromosome-type aberrations during G1, and (2) long-lived DNA lesions; the lesions persist until S phase and repair of the lesions is inhibited by caffeine, hydroxyurea and arabinofuranosyl cytosine in G2.
Matsuda
Y
Y
Division of Genetics, National Institute of Radiological Sciences, Chiba, Japan.
Maemori
M
M
Tobari
I
I
D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D002110:Caffeine / Q000494:pharmacology; D002453:Cell Cycle / Q000528:radiation effects; D002869:Chromosome Aberrations / Q000187:drug effects / Q000528:radiation effects; D003561:Cytarabine / Q000494:pharmacology; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects / Q000528:radiation effects*; D006918:Hydroxyurea / Q000494:pharmacology; D008297:Male; D008677:Metaphase / Q000187:drug effects / Q000528:radiation effects; D051379:Mice; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology; D013094:Spermatozoa / Q000528:radiation effects*; D015053:Zygote / Q000166:cytology*
D001549:Benzamides; D011838:Radiation-Sensitizing Agents; D003561:Cytarabine; D002110:Caffeine; C025160:3-aminobenzamide; D006918:Hydroxyurea