Analysis of DNA synthesis in herpes simplex virus infected cells by dual parameter flow cytometry.
The DNA incorporation of a thymidine analogue bromodeoxyuridine (BrdUrd) in herpes simplex virus type 2 (HSV-2) infected cervical cancer cell lines (CaSki and C-33 A) was studied by dual parameter flow cytometry. HSV-2 infection resulted in an exponential increase in DNA synthesis in the CaSki cells. In the less permissive C-33 A cells the proportion of DNA-synthesizing cells cycled during HSV-2 infection. The inhibition of viral DNA synthesis by phosphonoformate (PFA) was able to inhibit the virus induced changes in the CaSki but not in the C-33 A cells. In the C-33 A cells a part of the virus induced cellular DNA synthesis represents repair replication of cellular DNA.
Lehtinen
M
M
Department of Biomedical Sciences, University of Tampere, Finland.
Kulomaa
P
P
Kallioniemi
O P
OP
Paavonen
J
J
Leinikki
P
P
D001973:Bromodeoxyuridine; D002453:Cell Cycle; D004279:DNA, Viral / Q000096:biosynthesis*; D005434:Flow Cytometry; D006801:Humans; D007700:Kinetics; D018139:Simplexvirus / Q000235:genetics*; D014407:Tumor Cells, Cultured; D014779:Virus Replication
D004279:DNA, Viral; D001973:Bromodeoxyuridine