[Ultrastructural localization of leptospiral antigens by colloidal gold technique].
The rabbit anti-mouse antibodies were successfully labelled with colloidal gold. The distribution of leptospiral antigens in ultrastructure was researched with McAbs and labelled antibodies. It was found that colloidal gold particles were mainly distributed at the outer membrane of leptospiral strain O17 cells, which indicated that the antigens recognized by McAbs 1A7E7, and 2F9D4 were localized at the outer membrane of leptospiral cells. It was thought that colloidal technique would provide a method for research on the antigen distribution of leptospiral cells in ultrastructure.
Qing
G F
GF
Dai
B M
BM
Wang
Y P
YP
D000818:Animals; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000032:analysis*; D002462:Cell Membrane / Q000648:ultrastructure; D006048:Gold Colloid, Radioactive; D006168:Guinea Pigs; D007921:Leptospira interrogans / Q000276:immunology* / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D006048:Gold Colloid, Radioactive