A microtest plate assay of functional excitatory amino acid receptors.
A microtest plate assay of functional excitatory amino acid receptors present on cultured chick embryo retinal cells has been developed. It is based on measurements of excitatory amino acid-mediated increase in 22Na+ influx into retinal cells adhering to each of the 96 wells of microtest plates. Dose-dependent responses to L-glutamate, kainate and N-methyl-D-aspartate but not to quisqualate can be measured. These responses are selectively inhibited by antagonists of excitatory amino acids. The assay is reliable, fast to perform and parsimonious in terms of the volume and thus of amount of the drug applied. It allows a single investigator to perform, in one day, measurements of the effects of known or putative glutamatergic ligands in more than 100 different conditions.
Eshhar
N
N
Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel.
Lusky
M
M
David
P
P
Teichberg
V I
VI
D000818:Animals; D001681:Biological Assay; D002642:Chick Embryo; D017459:Receptors, Amino Acid; D011956:Receptors, Cell Surface / Q000378:metabolism* / Q000502:physiology; D012160:Retina / Q000196:embryology / Q000378:metabolism* / Q000502:physiology; D012964:Sodium / Q000502:physiology*
D017459:Receptors, Amino Acid; D011956:Receptors, Cell Surface; D012964:Sodium