The effects of p21N-ras expression in NIH-3T3 cells upon cyclic AMP metabolism.
The effects of overexpression of p21N-ras upon cyclic AMP metabolism have been examined in the inducible T15 cell line. In cells overexpressing the N-ras gene product, beta-adrenergic stimulation of cyclic AMP generation was reduced. The reduction was more pronounced the longer the ras gene was expressed and in chronically transformed cells a reduction in forskolin-stimulated cyclic AMP generation was also observed. The transformed cells exhibited a reduction in beta-adrenergic binding sites, but no change in the apparent EC50 for agonist induced cyclic AMP generation. Treatment of the cells with dibutyryl cyclic AMP induced a dose-dependent inhibition of proliferation, with the transformed cells being more sensitive than the control cells.
Davies
S A
SA
Department of Biochemistry, University of Glasgow, U.K.
Houslay
M D
MD
Wakelam
M J
MJ
D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D003994:Bucladesine / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002461:Cell Line, Transformed; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D003907:Dexamethasone / Q000494:pharmacology; D011905:Genes, ras; D007545:Isoproterenol / Q000494:pharmacology; D051379:Mice; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D016283:Proto-Oncogene Proteins p21(ras); D011943:Receptors, Adrenergic, beta / Q000187:drug effects / Q000235:genetics
D011518:Proto-Oncogene Proteins; D011943:Receptors, Adrenergic, beta; D005576:Colforsin; D003994:Bucladesine; D003907:Dexamethasone; D000242:Cyclic AMP; D016283:Proto-Oncogene Proteins p21(ras); D007545:Isoproterenol