Involvement of c-myc oncogene in lymphoma cell lines with no detectable chromosome rearrangement of band 8q24.
Two lymphoma cell lines of B-cell type were established from Japanese patients with diffuse small noncleaved cell lymphoma. Cytogenetic analysis revealed a 14q+ marker chromosome in both cell lines, and a t(8;14)(q24.1;q32.3) seemed most likely to have occurred. The chromosome 8 pair, however, had no abnormalities. Molecular analysis demonstrated c-myc amplification lacking gross rearrangement in one cell line and genetic rearrangement of c-myc at the first intron as well as aberrant sizes of c-myc mRNA in the other cell line. In the latter case, it is possible that a t(8;14)(q24.1;q32.3) was buried in an unrecognized complex translocation. A combination of cytogenetic and molecular studies to determine the precise nature of the 14q32 translocation is discussed.
Ohno
H
H
Department of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.
Fukuhara
S
S
Doi
S
S
Amakawa
R
R
Horii
M
M
Akiyama
Y
Y
Fukuda
W
W
Honjo
T
T
Sugiyama
T
T
Uchino
H
H
D000368:Aged; D015152:Blotting, Northern; D015139:Blotting, Southern; D002675:Child, Preschool; D002871:Chromosome Banding; D002883:Chromosomes, Human, Pair 14; D002898:Chromosomes, Human, Pair 8; D004262:DNA Restriction Enzymes; D005819:Genetic Markers; D006801:Humans; D007621:Karyotyping; D008223:Lymphoma / Q000235:genetics*; D008297:Male; D011519:Proto-Oncogenes; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
D005819:Genetic Markers; D004262:DNA Restriction Enzymes