Cyclic nucleotides and GTP analogues stimulate light-induced phosphorylation of octopus rhodopsin.
Light-induced phosphorylation of octopus rhodopsin in microvillar membrane was shown to be stimulated by cyclic nucleotides in contrast to vertebrate rhodopsin kinase. Non-hydrolyzable GTP analogues, GTP lambda S and GppNHp, greatly enhanced the light-induced phosphorylation of octopus rhodopsin, but the non-hydrolyzable GDP analogue, GDP beta S, was not effective. These results suggest that rhodopsin A-kinase is involved in regulating the interaction between rhodopsin and G-protein in octopus photoreceptors.
Tsuda
M
M
Department of Physics, Sapporo Medical College, Japan.
Tsuda
T
T
Hirata
H
H
D000818:Animals; D000242:Cyclic AMP / Q000494:pharmacology*; D006152:Cyclic GMP / Q000494:pharmacology*; D005136:Eye Proteins; D051606:G-Protein-Coupled Receptor Kinase 1; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate / Q000494:pharmacology; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology*; D007700:Kinetics; D008027:Light; D049831:Octopodiformes; D010766:Phosphorylation; D010786:Photoreceptor Cells / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism; D012168:Retinal Pigments / Q000378:metabolism*; D012243:Rhodopsin / Q000378:metabolism* / Q000528:radiation effects; D013873:Thionucleotides / Q000494:pharmacology*
D005136:Eye Proteins; D012168:Retinal Pigments; D013873:Thionucleotides; D006165:Guanylyl Imidodiphosphate; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate; D012243:Rhodopsin; D000242:Cyclic AMP; D011494:Protein Kinases; D051606:G-Protein-Coupled Receptor Kinase 1; D006152:Cyclic GMP