Tunicamycin modulates binding of 125I-erythropoietin to Friend erythroleukemia cells.
The effects of tunicamycin and neuraminidase treatment on the specific binding of 125I-erythropoietin to a murine erythroleukemia cell clone, B8, were investigated. Neuraminidase treatment of B8 cells did not affect the specific binding of erythropoietin, but tunicamycin treatment caused a 2.5 to 4-fold increase in the amount of 125I-erythropoietin binding. Scatchard analysis of the binding data showed that the increase in the amount of binding resulted from increased affinity of the receptor. These results suggest that N-linked sugars of the erythropoietin receptor protein are involved in the interaction of erythropoietin with the cell-surface receptors on B8 cells.
Fukamachi
H
H
Tojo
A
A
Saito
T
T
Kitamura
T
T
Urabe
A
A
Takaku
F
F
D000818:Animals; D002478:Cells, Cultured; D004921:Erythropoietin / Q000378:metabolism*; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism*; D051379:Mice; D011485:Protein Binding / Q000187:drug effects; D011956:Receptors, Cell Surface / Q000378:metabolism*; D017467:Receptors, Erythropoietin; D014415:Tunicamycin / Q000494:pharmacology*
D011956:Receptors, Cell Surface; D017467:Receptors, Erythropoietin; D014415:Tunicamycin; D004921:Erythropoietin