title: Two different strains of an alphaherpesvirus can establish latency in the same tissue of the host animal: evidence from bovine herpesvirus 1.
abstract: We inoculated plaque-purified bovine herpesvirus type 1 (BHV 1), strain K22, subtype BHV 1.2b, intravenously into susceptible cattle. Five months later, we reactivated latent virus with dexamethasone and then super-infected the same cattle intranasally and intravaginally with a different plaque-purified BHV 1, strain Cooper, subtype BHV 1.1. After a second dexamethasone treatment four months later, reactivated viruses were isolated and examined with restriction endonucleases. We showed that both virus subtypes were reactivated, proving that 2 different strains of an alphaherpesvirus can establish latency in the same tissue in the host animal.
authors.affiliation: National Animal Disease Center, USDA-Agricultural Research Service, Ames, Iowa.
authors.forename: C A
authors.initials: CA
authors.lastname: Whetstone
authors.forename: J M
authors.initials: JM
authors.lastname: Miller
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000302:isolation & purification; D003907:Dexamethasone / Q000008:administration & dosage; D007242:Herpesvirus 1, Bovine / Q000302:isolation & purification / Q000472:pathogenicity*; D007241:Infectious Bovine Rhinotracheitis / Q000382:microbiology; D008297:Male; D014762:Viral Interference; D014775:Virus Activation
chemical_list: D004279:DNA, Viral; D003907:Dexamethasone; D004262:DNA Restriction Enzymes