title: Characterization of two genome types of adenovirus type 31 isolated in Stockholm during 1987.
abstract: The authors isolated during 1987 seven adenovirus type 31 (Ad31) within a 9-month period. The isolates were obtained from urine, throat, and feces, implying a systemic spread of the infection. Most patients displayed gastrointestinal symptoms, but some had respiratory symptoms and fever. All of the strains differed by restriction endonuclease analysis from the prototype strain (1315) by an additional Bgl II restriction site. Ad31 isolates 1-6 could be divided into two groups by the enzymes Bam HI, Msp I, and Xho I. Each enzyme gave rise to the same group distribution: isolates 1-3 and 4-6, respectively. Digestion with Bst EII, Hind III, Kpn I, and Sma I resulted in identical patterns for isolates 1-6. Isolate 7, however, demonstrated a DNA deletion of approximately 0.8 kbp, but it was otherwise identical to isolates 4-6. In conclusion, two separate genome types of Ad31 were isolated, one of which included a DNA deletion mutant. The increased isolation rate may reflect an epidemiological situation, as the same isolation procedure had been used both before and after this period.
authors.affiliation: Department of Clinical Microbiology, Karolinska Hospital, Stockholm, Sweden.
authors.forename: M E
authors.initials: ME
authors.lastname: Johansson
authors.forename: M
authors.initials: M
authors.lastname: Brundin
authors.forename: L
authors.initials: L
authors.lastname: Adamson
authors.forename: L
authors.initials: L
authors.lastname: Grillner
authors.forename: M
authors.initials: M
authors.lastname: Landqvist
authors.forename: A
authors.initials: A
authors.lastname: Thörner
authors.forename: B Z
authors.initials: BZ
authors.lastname: Wirgart
mesh_terms: D000258:Adenovirus Infections, Human / Q000382:microbiology; D000260:Adenoviruses, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D000293:Adolescent; D000328:Adult; D002460:Cell Line; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification; D005260:Female; D005814:Genes, Viral; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D012703:Serotyping; D013548:Sweden
chemical_list: D004279:DNA, Viral; D004262:DNA Restriction Enzymes