title: Molecular cloning of the Na,K-ATPase alpha-subunit in developing brine shrimp and sequence comparison with higher organisms.
abstract: We report here the molecular cloning, nucleotide sequence, and predicted amino acid sequence of an alpha-subunit of the developmentally useful model, Artemia. The amino acid sequence shows divergence from that of mammals, birds, Torpedo, and Drosophila. However, regions in the putative ATP binding and transmembrane domains show absolute or high levels of conservation. Major differences occur in the amino-terminal domain and several other hypervariable regions. These differences are consistent with the suggestion that the brine shrimp is a 'fast clock' organism which diverged from the precursors of vertebrates 0.5-1 billion years ago.
authors.affiliation: Department of Pharmacology, University of Wisconsin Medical School, Madison 53706.
authors.forename: L A
authors.initials: LA
authors.lastname: Baxter-Lowe
authors.forename: J Z
authors.initials: JZ
authors.lastname: Guo
authors.forename: E E
authors.initials: EE
authors.lastname: Bergstrom
authors.forename: L E
authors.initials: LE
authors.lastname: Hokin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001156:Artemia / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D002645:Chickens; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004330:Drosophila / Q000201:enzymology / Q000235:genetics; D015723:Gene Library; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D012756:Sheep; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics*; D013045:Species Specificity; D014101:Torpedo
chemical_list: D004247:DNA; D000254:Sodium-Potassium-Exchanging ATPase