[Identification and characteristics of the beta-adrenergic receptors in the membranes of cultured endothelial cells of the human pulmonary artery].
The properties of beta-adrenoreceptors (beta-AR) have been studied in endothelial cells from the human pulmonary artery (EPA) and umbilical vein (EUV). [125I] Iodosyanopindolol binding assay revealed the amount of 22 and 12 fmol/10(6) cells as well as Kd = 92 and 52 pM for EPA and EUV, respectively. Adrenergic agonists increased the cAMP levels in EPA in the order of potency characteristic for beta 2-AR: isoproterenol greater than epinephrine greater than norepinephrine. Basing on the results obtained in the present study and the literature data a conclusion is made that the properties of beta-AR in vascular endothelial cells do not practically depend on their localization, suggesting universality of their function within the vascular system.
Grigorian
G Iu
GIu
Mirzopoiazova
T Iu
TIu
Nikashin
A V
AV
Goncharov
N V
NV
Danilov
S M
SM
D002395:Catecholamines / Q000494:pharmacology; D002462:Cell Membrane / Q000032:analysis / Q000187:drug effects; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000032:analysis; D004730:Endothelium, Vascular / Q000032:analysis* / Q000187:drug effects; D006801:Humans; D007457:Iodine Radioisotopes; D020120:Iodocyanopindolol; D010869:Pindolol / Q000031:analogs & derivatives; D011651:Pulmonary Artery / Q000032:analysis* / Q000187:drug effects; D011869:Radioligand Assay / Q000379:methods; D011943:Receptors, Adrenergic, beta / Q000032:analysis* / Q000187:drug effects; D014471:Umbilical Veins / Q000032:analysis / Q000187:drug effects
D002395:Catecholamines; D007457:Iodine Radioisotopes; D011943:Receptors, Adrenergic, beta; D020120:Iodocyanopindolol; D010869:Pindolol; D000242:Cyclic AMP
Identifikatsiia i kharakteristika beta-adrenergicheskikh retseptorov v membranakh kul'tiviruemykh kletok éndoteliia legochnoĭ arterii cheloveka.