Detection of HIV-1 DNA in different subsets of human peripheral blood mononuclear cells using the polymerase chain reaction. Rapid communication.
The presence of HIV-1 proviral DNA was determined in CD4 cells, CD8 cells and macrophages/monocytes obtained from peripheral blood of 8 HIV-1 infected persons. Using the polymerase chain reaction (PCR) we were able to detect proviral DNA in the extracts of only 10(2)-10(3) CD4 (T4) cells. In contrast, 10(5) CD8 cells did not contain detectable amounts of proviral DNA. Surprisingly, in four of our eight patients studied no HIV-1 DNA was found in macrophages. In peripheral blood, every hundred T4 cell may be infected with HIV-1.
Hufert
F T
FT
Virological Department, Bernhard-Nocht Institute of Tropical Medicine, Hamburg, Federal Republic of Germany.
von Laer
D
D
Schramm
C
C
Tárnok
A
A
Schmitz
H
H
D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology*; D015496:CD4-Positive T-Lymphocytes / Q000032:analysis / Q000382:microbiology; D004279:DNA, Viral / Q000032:analysis*; D004259:DNA-Directed DNA Polymerase; D005784:Gene Amplification; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000032:analysis* / Q000382:microbiology; D011533:Proviruses / Q000235:genetics / Q000302:isolation & purification; D050378:T-Lymphocytes, Regulatory / Q000032:analysis / Q000382:microbiology; D019914:Taq Polymerase
D004279:DNA, Viral; D019914:Taq Polymerase; D004259:DNA-Directed DNA Polymerase