title: Coexistence of acetylcholinesterase-, human growth hormone-releasing factor(1-37)-, alpha-melanotropin- and melanin-concentrating hormone-like immunoreactivities in neurons of the rat hypothalamus: a light and electron microscope study.
abstract: Using an antiserum (AS) raised against rat cerebral acetylcholinesterase (AChE), we revealed a neuron population in lateral and dorsal areas of the posterior rat hypothalamus. These neurons were previously described using antibodies to human growth hormone-releasing factor(1-37) (GRF-37), alpha-melanotropin (alpha-MSH) and melanin-concentrating hormone (MCH). Different intracytoplasmic distributions of the immunodeposits were observed depending on the used serum. Ultrastructural investigations demonstrated that AChE-AS labeled rough endoplasmic reticulum and nuclear envelope in control rats. MCH-AS stained Golgi apparatus in control animals and secretory granules in colchicine-injected rats. GRF-37-AS always revealed secretory granules, and alpha-MSH-AS gave the same staining only after colchicine injection.
authors.affiliation: CNRS URA 561, Laboratoire d'Histologie Embryologie Cytogénétique, Faculté de Médecine, Besançon, France.
authors.forename: P Y
authors.initials: PY
authors.lastname: Risold
authors.forename: D
authors.initials: D
authors.lastname: Fellmann
authors.forename: D
authors.initials: D
authors.lastname: Lenys
authors.forename: C
authors.initials: C
authors.lastname: Bugnon
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D004721:Endoplasmic Reticulum / Q000378:metabolism / Q000648:ultrastructure; D006056:Golgi Apparatus / Q000378:metabolism / Q000648:ultrastructure; D013007:Growth Hormone-Releasing Hormone / Q000378:metabolism*; D007028:Hypothalamic Hormones; D007031:Hypothalamus / Q000378:metabolism* / Q000648:ultrastructure; D007150:Immunohistochemistry; D008543:Melanins / Q000378:metabolism*; D008854:Microscopy, Electron; D010907:Pituitary Hormones / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D000521:alpha-MSH / Q000378:metabolism*
chemical_list: D007028:Hypothalamic Hormones; D008543:Melanins; D010907:Pituitary Hormones; D000521:alpha-MSH; C019463:melanin-concentrating hormone; D013007:Growth Hormone-Releasing Hormone; D000110:Acetylcholinesterase