title: The site of substrate and fructose 2,6-bisphosphate binding to rabbit liver fructose-1,6-bisphosphatase.
abstract: The binding site(s) in rabbit liver fructose-1,6-bisphosphatase for the active site binding ligand, fructose 6-phosphate, and the inhibitor, fructose 2,6-bisphosphate, have been investigated by using nuclear magnetic resonance spectroscopy. The distance from a nitroxide spin label to the bound ligands and the distance from the structural metal site to the bound ligands are about the same within experimental error. These data indicate that the two ligands probably bind at the active site in the rabbit liver enzyme.
authors.affiliation: Department of Biochemistry and Biophysics, Iowa State University, Ames 50011.
authors.forename: F
authors.initials: F
authors.lastname: Liu
authors.forename: M
authors.initials: M
authors.lastname: Roy
authors.forename: H J
authors.initials: HJ
authors.lastname: Fromm
mesh_terms: D000818:Animals; D001665:Binding Sites; D004228:Dithionitrobenzoic Acid; D006597:Fructose-Bisphosphatase / Q000378:metabolism*; D005636:Fructosephosphates / Q000378:metabolism*; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology*; D009682:Magnetic Resonance Spectroscopy; D011817:Rabbits; D013113:Spin Labels
chemical_list: D005636:Fructosephosphates; D013113:Spin Labels; D004228:Dithionitrobenzoic Acid; D006597:Fructose-Bisphosphatase