Treatment of encephalomyocarditis virus-induced central nervous system demyelination with monoclonal anti-T-cell antibodies.
Infection of BALB/c mice with the M variant of encephalomyocarditis virus resulted in the development of a paralytic syndrome in 7 to 10 days. The paralysis was maximal during the period of viral clearance; most of the animals recovered from the initial deficit and showed no delayed recurrences. Pathologically, the white matter of brain and spinal cord showed well-demarcated areas of perivascular cuffing, demyelination, and, during recovery, remyelination by oligodendrocytes--all suggestive of postinfectious encephalomyelitis. Depletion of either the CD4 or CD8 subset of T cells in vivo with the appropriate monoclonal antibody, GK1.5 or 2.43, respectively, administered one day (24 h) prior to infection was sufficient to limit the development of the paralytic syndrome by 79% (GK1.5) and 82% (2.43).
Sriram
S
S
Department of Neurology, University of Vermont, Burlington 05405.
Topham
D J
DJ
Huang
S K
SK
Rodriguez
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D000914:Antibodies, Viral / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D016827:CD8 Antigens; D003711:Demyelinating Diseases / Q000209:etiology / Q000473:pathology / Q000628:therapy*; D004686:Encephalomyocarditis virus / Q000276:immunology / Q000302:isolation & purification; D004769:Enterovirus Infections / Q000209:etiology / Q000473:pathology / Q000628:therapy*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D010243:Paralysis / Q000209:etiology; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000945:Antigens, Differentiation, T-Lymphocyte; D016827:CD8 Antigens