title: Coordinated V gamma and V delta gene segment rearrangements in human T cell receptor gamma/delta+ lymphocytes.
abstract: Monoclonal antibodies (mAb) were used to characterize a panel (n = 46) of T cell receptor (TcR) gamma/delta+ T cell clones. Three of these antibodies have been described to react with specific variable region-encoded protein products and can therefore be used to detect functional gene rearrangements. The majority of peripheral blood-derived clones (43 out of 45) expressed the epitopes recognized by mAb BB3, encoded by the V delta 2 gene segment and mAb Ti gamma A, encoded by the V gamma 9 gene segment. These clones lacked the antigenic determinant recognized by mAb delta-TCS-1, encoded by the V delta 1 gene segment. The other two peripheral blood-derived clones and an ascites-derived clone were Ti gamma A-, BB3- and delta-TCS-1+. Biochemical analysis revealed that all Ti gamma A+, BB3+ T cell clones expressed the disulfide-linked form of the receptor. The two peripheral blood-derived delta-TCS-1+ T cell clones expressed the nondisulfide-linked form whereas the ascites-derived delta-TCS-1+ clone, AK119 expressed the disulfide-linked form of the TcR gamma/delta heterodimer. This indicates that V delta 1-encoded delta chains can be associated either with a C gamma 1- or a C gamma 2-encoded gamma chain. The preferential use of certain V gamma and V delta gene segments suggests the existence of a limited combinatorial diversity in TcR gamma/delta heterodimers, i.e. Ti gamma A+ (V gamma 9), BB3+ (V delta 2) and delta-TCS-1- disulfide-linked heterodimers and Ti gamma A-, BB3- and delta-TCS-1+ (V delta 1) disulfide- or non disulfide-linked forms.
authors.affiliation: Department of Immunology, Dr. Daniel den Hoed Cancer Center, Rotterdam, The Netherlands.
authors.forename: E
authors.initials: E
authors.lastname: Sturm
authors.forename: E
authors.initials: E
authors.lastname: Braakman
authors.forename: R E
authors.initials: RE
authors.lastname: Bontrop
authors.forename: P
authors.initials: P
authors.lastname: Chuchana
authors.forename: R J
authors.initials: RJ
authors.lastname: Van de Griend
authors.forename: F
authors.initials: F
authors.lastname: Koning
authors.forename: M P
authors.initials: MP
authors.lastname: Lefranc
authors.forename: R L
authors.initials: RL
authors.lastname: Bolhuis
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D015139:Blotting, Southern; D004220:Disulfides; D015329:Gene Rearrangement, T-Lymphocyte; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D006801:Humans; D011233:Precipitin Tests; D011948:Receptors, Antigen, T-Cell / Q000235:genetics* / Q000276:immunology; D016692:Receptors, Antigen, T-Cell, gamma-delta; D013601:T-Lymphocytes / Q000502:physiology*
chemical_list: D000911:Antibodies, Monoclonal; D004220:Disulfides; D011948:Receptors, Antigen, T-Cell; D016692:Receptors, Antigen, T-Cell, gamma-delta