Preliminary purification and characterization studies of a low molecular weight, high affinity cytosolic lead-binding protein in rat brain.
Carrier-free 203Pb has been used to label high affinity lead-binding proteins in rat brain cytosol to allow their initial characterization. The low molecular weight 203Pb-protein complex collected from a Sephadex G-75 column eluate has been further purified by Sephadex DEAE chromatography and then partially characterized. The protein has a molecular weight of 23,000 daltons as determined by SDS polyacrylamide gel electrophoresis and significant levels of glutamic acid (9.3%), aspartic acid (10.8%) and cysteine (9.4%). Western blot studies conducted using the polyclonal antibody to the renal lead-binding proteins showed a lack of reactivity, indicating that the brain protein is immunologically distinct from that found in the kidney.
DuVal
G
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Department of Pathology, University of Maryland School of Medicine, Baltimore 21201.
Fowler
B A
BA
D000818:Animals; D001224:Aspartic Acid / Q000032:analysis; D015153:Blotting, Western; D001923:Brain Chemistry; D002352:Carrier Proteins / Q000302:isolation & purification* / Q000378:metabolism; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003545:Cysteine / Q000032:analysis; D003600:Cytosol / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005971:Glutamates / Q000032:analysis; D018698:Glutamic Acid; D007854:Lead / Q000378:metabolism; D008099:Liver / Q000032:analysis; D008297:Male; D008970:Molecular Weight; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains
D002352:Carrier Proteins; D005971:Glutamates; C055217:lead-binding proteins; D007854:Lead; D001224:Aspartic Acid; D018698:Glutamic Acid; D003545:Cysteine