Dibutyryl guanosine cyclic monophosphate causes proliferation of interleukin-2-dependent T-cells in the presence of suboptimal levels of interleukin-2.
The effect of dibutyryl guanosine cyclic monophosphate (db-cGMP) on proliferation of an interleukin-2 (IL-2)-dependent T-cell line was investigated. Dibutyryl cGMP added to HT-2 cell cultures without IL-2 or in the presence of an optimal IL-2 concentration (60 U/ml) had no effect on proliferation. The same amount of db-cGMP (10(-5)M) added to cultures in the presence of suboptimal amounts of IL-2 (3-6 U/ml) produced significant increases in proliferation. In contrast, db-cAMP caused substantial inhibition of HT-2 cells cultured with IL-2. Similar effects were observed using human T-cell blasts produced after 72 h stimulation with phytohemagglutin. These results suggest that cyclic GMP may play a role in the cellular response of IL-2 responsive cells to IL-2.
Kucharz
E J
EJ
Department of Medicine, University of Wisconsin School of Medicine, Milwaukee 53233.
Goodwin
J S
JS
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D006152:Cyclic GMP; D003995:Dibutyryl Cyclic GMP / Q000494:pharmacology*; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000494:pharmacology / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*
D007376:Interleukin-2; D003995:Dibutyryl Cyclic GMP; D003994:Bucladesine; D006152:Cyclic GMP