Control of fructose 2,6-bisphosphate levels in rat macrophages by glucose and phorbol ester.
The presence of fructose 2,6-bisphosphate (Fru 2,6-P2) in elicited peritoneal macrophages of rat was examined. These cells possess an active phosphofructokinase-2 which is diminished by citrate and only slightly inhibited by glycerol 3-phosphate. Phosphofructokinase-1 submaximal activity was increased 26-fold by the addition of 1 microM Fru 2,6-P2. Incubation of cells without glucose decreased the amount of Fru 2,6-P2 to zero, but further addition of 5 mM glucose increased the levels of the sugar ester 20-fold. In addition, the presence of phorbol ester potentiated the synthesis of Fru 2,6-P2. By contrast phenylisopropyladenosine or prostaglandin F2 alpha inhibited the production of Fru 2,6-P2.
Bustos
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Departamento de Bioquímica Médica y Biología Molecular, Facultad de Medicina, Universidad de Sevilla, Spain.
Sobrino
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D000818:Animals; D002951:Citrates / Q000494:pharmacology; D019343:Citric Acid; D000242:Cyclic AMP / Q000378:metabolism; D015237:Dinoprost / Q000494:pharmacology; D005635:Fructosediphosphates / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology*; D006003:Glycogen / Q000378:metabolism; D006598:Hexosediphosphates / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010660:Phenylisopropyladenosine / Q000494:pharmacology; D010732:Phosphofructokinase-1 / Q000037:antagonists & inhibitors / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D002951:Citrates; D005635:Fructosediphosphates; D006598:Hexosediphosphates; D007773:Lactates; D010660:Phenylisopropyladenosine; D019343:Citric Acid; D019344:Lactic Acid; C027652:fructose 2,6-diphosphate; D006003:Glycogen; D015237:Dinoprost; D000242:Cyclic AMP; D010732:Phosphofructokinase-1; D005947:Glucose; D013755:Tetradecanoylphorbol Acetate