title: Post-ultraviolet DNA synthesis in the absence of repair: role of the single-strand DNA-binding protein.
abstract: Post-ultraviolet DNA synthesis kinetics were investigated in the Escherichia coli uvrA recA strain and its isogenic counterpart, overproducing single-strand DNA-binding protein (SSB). It was demonstrated that large quantities of SSB enhance the capacity of the unmodified replisome to use the UV-damaged template for DNA synthesis. DNA thus synthesized is of low molecular weight, as shown by sedimentation in alkaline sucrose gradients. It is therefore suggested that SSB actively participates in the replisome translocation past dimers and/or the initiation of new DNA chains downstream of these lesions.
authors.affiliation: Ruder Bosković Institute, Zagreb, Yugoslavia.
authors.forename: Z
authors.initials: Z
authors.lastname: Trgovcević
authors.forename: N
authors.initials: N
authors.lastname: Lers
authors.forename: K
authors.initials: K
authors.lastname: Brcić-Kostić
authors.forename: E
authors.initials: E
authors.lastname: Salaj-Smic
mesh_terms: D004260:DNA Repair; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004268:DNA-Binding Proteins / Q000502:physiology*; D004926:Escherichia coli / Q000528:radiation effects*; D014466:Ultraviolet Rays
chemical_list: D004269:DNA, Bacterial; D004268:DNA-Binding Proteins