title: Investigations on the influence of copper succinate on the production of superoxide anion radicals by bovine small intestinal mucosa cells.
abstract: The effect of an experimental ischemia lasting for 45 minutes and a subsequent period of reperfusion of equal length on the activity of xanthine oxidase (XO) and microsomal NADPH-cytochrome P450 reductase (NADPH-CR) were investigated in the small intestinal mucosa of male neonatal calves of the breed German "Schwarzbunte". The activity of the NADPH-CR was determined by chemiluminescence. The activity of XO decreased during ischemia, but rose to values above the control level following reperfusion. 5 mg of Cu2(succinate)2 (CuSu) administered either intraarterially or intraluminally during reperfusion prevented the rise in XO. Formation of malondialdehyde decreased in the presence of CuSu. The NADPH-CR likewise showed subnormal activity values during ischemia, but also remained at a low level during reperfusion. The activity of this enzyme was further lowered by local intraarterial or intraluminal administration of 5 mg of CuSu and by 120 mg of CuSu administered intravenously during the reperfusion. These results are discussed with regard to the superoxide anion radical induced tissue lesions observed during reperfusion.
authors.forename: G
authors.initials: G
authors.lastname: Harisch
authors.forename: M
authors.initials: M
authors.lastname: Kretschmer
authors.forename: T
authors.initials: T
authors.lastname: Richter
authors.forename: M
authors.initials: M
authors.lastname: Pickel
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control*; D003300:Copper / Q000627:therapeutic use*; D007413:Intestinal Mucosa / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism*; D008297:Male; D015427:Reperfusion Injury / Q000517:prevention & control / Q000662:veterinary*; D013386:Succinates / Q000627:therapeutic use*; D019802:Succinic Acid; D013481:Superoxides / Q000378:metabolism*
chemical_list: D013386:Succinates; D013481:Superoxides; D003300:Copper; D019802:Succinic Acid