title: Identification of serotype 9 human rotavirus by enzyme-linked immunosorbent assay with monoclonal antibodies.
abstract: Hybridomas producing monoclonal antibodies to VP7, a major neutralizing protein of serotype 9 rotavirus (strain W161), were prepared. One monoclonal antibody, W161-6A1, was shown to neutralize only serotype 9 rotavirus strains and reacted specifically with serotype 9 rotaviruses in an enzyme-linked immunosorbent assay. The development of an immunoassay for detection of serotype 9 rotaviruses should facilitate epidemiologic studies.
authors.affiliation: Laboratory of Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
authors.forename: K
authors.initials: K
authors.lastname: Midthun
authors.forename: J
authors.initials: J
authors.lastname: Valdesuso
authors.forename: A Z
authors.initials: AZ
authors.lastname: Kapikian
authors.forename: Y
authors.initials: Y
authors.lastname: Hoshino
authors.forename: K Y
authors.initials: KY
authors.lastname: Green
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D002213:Capsid / Q000276:immunology*; D036022:Capsid Proteins; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D012401:Rotavirus / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; C050779:VP7 protein, Rotavirus