Linkage analysis in familial adenomatous polyposis: order of C11P11 (D5S71) and pi 227 (D5S37) loci at the apc gene.
Linkage analysis with DNA probes C11P11 and pi 227 is reported in six Scottish families with familial adenomatous polyposis. Two families were informative for C11P11 and all six were at least partly informative for pi 227. Two C11P11-apc and two pi 227-apc recombinants were identified and one of these was recombinant for both C11P11-apc and pi 227-apc. A further possible combined C11P11-apc and pi 227-apc recombination event was also identified. Peak lod score for linkage of C11P11 to apc was 5.80 at a recombination fraction (theta) of 0.069 (95% probability limits 0.012-0.191) and for linkage of pi 227 to apc was 3.19 at theta = 0.110 (95% probability limits 0.023-0.286). Peak lod score for linkage of C11P11 to pi 227 was 1.79 at theta = 0.00. The data support a gene order of pi 227-C11P11-apc.
Dunlop
M G
MG
MRC Human Genetics Unit, Western General Hospital, Edinburgh, Scotland.
Steel
C M
CM
Wyllie
A H
AH
Bird
C C
CC
Evans
H J
HJ
D002895:Chromosomes, Human, Pair 5; D015342:DNA Probes; D008040:Genetic Linkage; D006801:Humans; D007621:Karyotyping; D008126:Lod Score; D009377:Multiple Endocrine Neoplasia / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D011995:Recombination, Genetic
D015342:DNA Probes