Different gamma-glutamyl transpeptidase mRNAs are expressed in human liver and kidney.
In human, the two subunits of gamma-glutamyl transpeptidase (GGT) arise from a common precursor encoded by a multigene family. Until now, a single specific coding sequence for this precursor (type I) has been identified in human placenta and liver. In the present study, we have isolated from a human kidney cDNA library, a GGT specific clone (0.8 Kb). The sequence of which (type II) i) covers the carboxy terminal part of the GGT precursor, ii) exhibits 22 point mutations and a 30 bp deletion as compared to the type I GGT sequence. The sequencing of a human genomic clone reveals that this type II GGT mRNA is encoded by a different gene than the type I GGT mRNA. Both type I and type II GGT mRNAs are expressed in human liver, while almost exclusively type II GGT mRNA is detected in human kidney.
Pawlak
A
A
Unité INSERM 99, Hôpital Henri Mondor, Creteil, France.
Wu
S J
SJ
Bulle
F
F
Suzuki
A
A
Chikhi
N
N
Ferry
N
N
Baik
J H
JH
Siegrist
S
S
Guellaën
G
G
D000328:Adult; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D005796:Genes; D015698:Genomic Library; D006801:Humans; D007527:Isoenzymes / Q000235:genetics*; D007668:Kidney / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics*; D015183:Restriction Mapping; D005723:gamma-Glutamyltransferase / Q000235:genetics*
D007527:Isoenzymes; D012333:RNA, Messenger; D004247:DNA; D005723:gamma-Glutamyltransferase