[Isolation of nucleotide sequences, possessing enhancer functions from the Saccharomyces cerevisiae genetic library].
We propose a method for isolation of enhancer-like sequences from a yeast genomic library. The method was used to identify DNA inserts capable of increasing bacterial bla gene expression when located downstream from the transcription initiation site. Plasmids carrying different fragments of one such insert were used to localize the enhancer-like function on a 1.2 kb fragment.
Polishchuk
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Nevzgliadova
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Tereshin
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Soĭdla
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D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004587:Electrophoresis, Agar Gel; D004742:Enhancer Elements, Genetic; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D005800:Genes, Fungal; D005814:Genes, Viral; D006868:Hydrolysis; D010957:Plasmids; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D014170:Transformation, Genetic; D001618:beta-Lactamases / Q000378:metabolism
D004251:DNA Transposable Elements; D001618:beta-Lactamases
Vydelenie nukleotidnykh posledovatel'nosteĭ, obladaiushchikh énkhansernoĭ funktsieĭ, iz genoteki Saccharomyces cerevisiae.