title: Dynamic properties of the Ca2+/calmodulin-dependent protein kinase in Drosophila: identification of a synapsin I-like protein.
abstract: Visual adaptation with blue light induces a change in a special light/dark choice behavior in Drosophila. On the molecular level adaptation induces long-term modulation of the in vitro autophosphorylation capacity of a Ca2+/calmodulin-dependent protein kinase. Here I describe a Drosophila phosphoprotein that is a substrate of this protein kinase. The molecular mass and phosphopeptide composition of this protein are similar to those of rat synapsin I. Furthermore, the Drosophila protein shows immunological cross-reactivity with monoclonal antibodies against rat synapsin I. I conclude that this 86-kDa protein in Drosophila is homologous to the vertebrate synapsin I.
authors.affiliation: Institut für Biologie III der Universität Freiburg, Federal Republic of Germany.
authors.forename: H
authors.initials: H
authors.lastname: Mitschulat
mesh_terms: D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D002462:Cell Membrane / Q000201:enzymology; D003488:Cyanogen Bromide; D004330:Drosophila / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000032:analysis*; D010449:Peptide Mapping; D010750:Phosphoproteins / Q000032:analysis*; D010766:Phosphorylation; D010775:Photic Stimulation; D011494:Protein Kinases / Q000378:metabolism*; D012160:Retina / Q000502:physiology; D016704:Synapsins
chemical_list: D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; D016704:Synapsins; D011494:Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D003488:Cyanogen Bromide