[A non-enzymatic method of preparing isolated hepatocytes: assessment of their morpho-functional activity].
A method for isolating hepatocytes using the two-step liver perfusion by chelate-containing solutions is described. The hepatocytes obtained are highly inactive for the trypan blue staining test, (not less than 90%), ATP content, the rate of endogenic respiration and its stimulation by succinate, a degree of xenobiotic oxidation.
Kravchenko
L P
LP
Andrienko
A N
AN
Petrenko
A Iu
AIu
Sukach
A N
AN
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002469:Cell Separation; D002614:Chelating Agents; D005557:Formaldehyde / Q000378:metabolism; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism; D008297:Male; D010477:Perfusion; D051381:Rats
D002614:Chelating Agents; D005557:Formaldehyde; D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase; D005952:Glucose-6-Phosphatase
Nefermentativnyĭ metod polucheniia izolirovannykh gepatotsitov: otsenka ikh morfo-funktsional'noĭ aktivnosti.