title: Characterization of human IFN-gamma response using somatic cell hybrids of hematopoietic and nonhematopoietic origin.
abstract: A panel of 27 rodent-human somatic cell hybrids composed of cells of hematopoietic (nonadherent cells) and nonhematopoietic origin (adherent cells) was used to identify the chromosomes involved in the biological response to human IFN-gamma (Hu-IFN-gamma). We found that the stimulation of class-I histocompatibility antigen expression correlates with the presence of human chromosomes 6 and 21 in adherent cell hybrids, while human chromosome 6 alone is sufficient in nonadherent hybrids. Scatchard analysis of the binding of radiolabeled Hu-IFN-gamma to nonadherent cell hybrids gave a Kd value similar to that found on human cell lines. Induction of a reporter gene placed under the transcriptional control of the interferon responsive sequence (IRS) in adherent cell hybrids requires both chromosomes 6 and 21. The antiviral protection by Hu-IFN-gamma in adherent cell hybrids was reached at physiological doses (2 units/ml) when human chromosomes 6 and 21 were present, while higher doses of Hu-IFN-gamma (5000 units/ml) were required for hybrids lacking chromosome 21. Thus, we demonstrate that differences exit in the response to Hu-IFN-gamma depending on the origin of the cell type.
authors.affiliation: Institut Pasteur-INSERM U.276.
authors.forename: M R
authors.initials: MR
authors.lastname: Bono
authors.forename: P
authors.initials: P
authors.lastname: Benech
authors.forename: P
authors.initials: P
authors.lastname: Couillin
authors.forename: C
authors.initials: C
authors.lastname: Alcaide-Loridan
authors.forename: M C
authors.initials: MC
authors.lastname: Grisard
authors.forename: H
authors.initials: H
authors.lastname: Jouin
authors.forename: D G
authors.initials: DG
authors.lastname: Fischer
authors.forename: M
authors.initials: M
authors.lastname: Fellous
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002460:Cell Line; D002891:Chromosomes, Human, Pair 21; D002896:Chromosomes, Human, Pair 6; D004351:Drug Resistance / Q000235:genetics; D005455:Fluorescent Antibody Technique; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism*; D004854:Herpesvirus 4, Human; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis / Q000276:immunology; D006801:Humans; D006822:Hybrid Cells; D007371:Interferon-gamma / Q000494:pharmacology*; D014162:Transfection; D014721:Vesicular stomatitis Indiana virus
chemical_list: D000911:Antibodies, Monoclonal; D015395:Histocompatibility Antigens Class I; D007371:Interferon-gamma