Effect of WR-2721 on radiation-induced lipid peroxidation and enzyme release in erythrocytes and microsomes.
The effect of WR-2721 on radiation-induced lipid peroxidation and enzyme release was studied after gamma-irradiation of microsomes and erythrocytes. WR-2721 rendered protection to microsomes by reducing lipid peroxidation whereas it did not exert significant protection against radiation-induced lipid peroxidation in erythrocytes. The non-protective effect of WR-2721 was also observed in radiation-induced enzyme (AChE) release of erythrocytes. It is proposed that erythrocytes do not dephosphorylate WR-2721 into the free thiol derivative (WR-1065). This was confirmed by the microsomal treatment of erythrocytes that had shown the protective effect of WR-2721. Further, a significant increase in sulphydryl content in microsomes, and no such increase in erythrocytes, after the WR-2721 treatment indicated that dephosphorylation of WR-2721 is necessary for its protective effect.
Ayene
S I
SI
Radiation Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
Srivastava
P N
PN
D000110:Acetylcholinesterase / Q000378:metabolism; D004999:Amifostine / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology; D004912:Erythrocytes / Q000201:enzymology / Q000528:radiation effects*; D015227:Lipid Peroxidation / Q000528:radiation effects*; D008861:Microsomes / Q000378:metabolism / Q000528:radiation effects*; D009946:Organothiophosphorus Compounds / Q000494:pharmacology*
D009946:Organothiophosphorus Compounds; D004492:Edetic Acid; D000110:Acetylcholinesterase; D004999:Amifostine