Phosphate regulon in members of the family Enterobacteriaceae: comparison of the phoB-phoR operons of Escherichia coli, Shigella dysenteriae, and Klebsiella pneumoniae.
The structure and function of the phoB and phoR genes of Shigella dysenteriae strains and Klebsiella pneumoniae, which are involved in regulation of the phosphate regulon, were analyzed. Complementation tests among the genes of Escherichia coli, S. dysenteriae strains, and K. pneumoniae for production of alkaline phosphatase indicate that S. dysenteriae serotype 2 and serotype 3 strains and K. pneumoniae are phoA+ phoB+ phoR+ but S. dysenteriae Sh and serotype 1 strains are phoA phoB+ phoR. Nucleotide sequences of phoB and phoR of S. dysenteriae Sh and K. pneumoniae are highly homologous to those of E. coli, except for a single base insertion found in phoR of S. dysenteriae Sh.
Lee
T Y
TY
Department of Experimental Chemotherapy, Osaka University, Japan.
Makino
K
K
Shinagawa
H
H
Amemura
M
M
Nakata
A
A
D000469:Alkaline Phosphatase / Q000235:genetics; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004755:Enterobacteriaceae / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005809:Genes, Regulator; D007711:Klebsiella pneumoniae / Q000235:genetics*; D008969:Molecular Sequence Data; D009876:Operon; D028044:Phosphate-Binding Proteins; D010710:Phosphates / Q000378:metabolism*; D010957:Plasmids; D018272:Porins; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D012762:Shigella dysenteriae / Q000235:genetics*
D001425:Bacterial Outer Membrane Proteins; D002352:Carrier Proteins; D004269:DNA, Bacterial; D028044:Phosphate-Binding Proteins; D010710:Phosphates; D018272:Porins; D000469:Alkaline Phosphatase