Chemical composition of phase I Coxiella burnetii soluble antigen prepared by trichloroacetic acid extraction.
Optimal conditions of extraction (time and temperature) by trichloroacetic acid of soluble antigen from phase I Coxiella burnetii (TCAE), possessing protective properties and used as a chemovaccine against Q fever in men, were studied. Extracts prepared under various conditions were analysed for their polysaccharide, protein and phosphorus contents. Forty-five min of extraction at 0 degrees C were sufficient to obtain a soluble antigen reacting in immunodiffusion with hyperimmune rabbit antiserum. The polysaccharide contents decreased with prolonged extraction at 0 degrees C. At higher extraction temperatures (37 and 100 degrees C), the polysaccharide contents increased while that of proteins decreased. TCAE prepared at 100 degrees C gave no positive immunodiffusion reaction.
Lukácová
M
M
Institute of Virology, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Brezina
R
R
Schramek
S
S
Pastorek
J
J
D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D003381:Coxiella / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005779:Immunodiffusion; D007700:Kinetics; D010758:Phosphorus / Q000032:analysis; D011134:Polysaccharides / Q000032:analysis*; D011135:Polysaccharides, Bacterial / Q000276:immunology / Q000302:isolation & purification*; D013696:Temperature; D013997:Time Factors; D014238:Trichloroacetic Acid
D001426:Bacterial Proteins; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; D010758:Phosphorus; D014238:Trichloroacetic Acid