Localization of the mouse gene for secreted phosphoprotein 1 (Spp-1) (2ar, osteopontin, bone sialoprotein 1, 44-kDa bone phosphoprotein, tumor-secreted phosphoprotein) to chromosome 5, closely linked to Ric (Rickettsia resistance).
We have used a cDNA probe for mouse secreted phosphoprotein 1 (Spp-1, also known as 2ar, osteopontin, bone sialoprotein 1, 44-kDa bone phosphotein, tumor-secreted protein) to find a restriction fragment length polymorphism in the gene from C57BL/6J and DBA/2J mice. The strain distribution pattern in 25 BXD recombinant inbred lines is identical with that previously determined for the dominant, autosomal gene, Ricr (Rickettsia resistance). This places Spp-1 on mouse chromosome 5 with a 95% confidence limit of being within 4.32 cM of Ric. Evidence supporting the possibility of allelism between Spp-1 and Ric is briefly discussed.
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Department of Cell Biology, Vanderbilt University Medical School, Nashville, Tennessee 37232-2175.
Dickinson
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Hogan
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D000818:Animals; D015342:DNA Probes; D051379:Mice / Q000235:genetics*; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D053495:Osteopontin; D010750:Phosphoproteins / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D012282:Rickettsia Infections / Q000235:genetics*; D012795:Sialoglycoproteins / Q000235:genetics*
D015342:DNA Probes; D010750:Phosphoproteins; D012795:Sialoglycoproteins; C506896:Spp1 protein, mouse; D053495:Osteopontin