title: A novel primase-free form of murine DNA polymerase alpha induced by infection with minute virus of mice.
abstract: Two species of DNA polymerase alpha free of primase activity were identified in extracts of Ehrlich mouse cells that had been infected with minute virus of mice. Primase-free forms of DNA polymerase alpha eluted with 150 and 180 mM NaCl during ion-exchange chromatography on DEAE-cellulose columns, exhibited sedimentation coefficients of 11 S and 8.2 S, respectively, and were inhibited by aphidicolin, N2-(p-n-butylphenyl)-9-(2-deoxy-beta-D-ribofuranosyl)guanine 5'-triphosphate, and 2-(p-n-butylanilino)-9-(2-deoxy-beta-D-ribofuranosyl)adenine 5'-triphosphate. The ratio of primase-free DNA polymerase alpha to the DNA polymerase alpha-primase complex increased from 1.5 to greater than 100 during the course of infection, and free primase was produced during the MVM replicative cycle.
authors.affiliation: Cancer Research Laboratory, University of Western Ontario, London, Canada.
authors.forename: T F
authors.initials: TF
authors.lastname: Ho
authors.forename: J S
authors.initials: JS
authors.lastname: Gupta
authors.forename: E A
authors.initials: EA
authors.lastname: Faust
mesh_terms: D000818:Animals; D016590:Aphidicolin; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology* / Q000382:microbiology; D002460:Cell Line; D002852:Chromatography, Ion Exchange / Q000379:methods; D004257:DNA Polymerase II / Q000235:genetics / Q000302:isolation & purification*; D019915:DNA Primase; D004279:DNA, Viral / Q000378:metabolism; D004224:Diterpenes / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D051379:Mice; D008915:Minute Virus of Mice / Q000235:genetics; D009154:Mutation; D010322:Parvoviridae Infections / Q000201:enzymology* / Q000235:genetics; D012316:RNA Nucleotidyltransferases / Q000032:analysis*; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D004224:Diterpenes; D016590:Aphidicolin; D019915:DNA Primase; D012316:RNA Nucleotidyltransferases; D004257:DNA Polymerase II