Long-term culture of peripheral blood stem cells: the effect of the addition of an irradiated stromal layer.
We have studied peripheral blood stem cells (PBSC) collected by cytapheresis following intensive chemotherapy, from 13 patients with acute leukemia, in long term culture (LTC). Peripheral blood was cultured with (n = 10) and without (n = 21) the addition of a preformed, irradiated stromal layer. In this latter LTC our results confirm that peripheral blood is capable of producing CFU-GM and nucleated cells in the absence of the formation of an adherent stromal layer. However, peripheral blood cultured in the presence of an irradiated stromal layer is capable of a significantly higher proliferative response (total production of CFU GM per flask - mean = 57529) than in the absence of an irradiated stromal layer (total production of CFU GM per flask - mean = 26739, p less than 0.03). Our results suggest that PBSC contain a primitive nonplastic adherent cell that requires the presence of a stromal layer for its expression. These findings provide further support for the use of peripheral blood stem cells for autologous transplantation.
Rice
A
A
Laboratory of Hematology, Hopital Haut Lévêque, CHR Bordeaux, France.
Bernard
P
P
Foures
C
C
Bascans
E
E
Lacombe
F
F
Marit
G
G
Reiffers
J
J
D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003470:Culture Media; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007937:Leukapheresis; D013997:Time Factors; D014182:Transplantation, Autologous
D003470:Culture Media