Glycogen-bound type-1 phosphatase: isolation and dissociation of a complex containing undegraded G-subunit.
A high molecular mass type-1 phosphatase complex can be isolated from muscle glycogen particles by a fast procedure that preserves the glycogen-binding subunit of phosphatase called G from proteolysis. G can be dissociated from such complex by ion exchange chromatography on FPLC SI column, with recovery of unproteolyzed G completely separated from phosphatase catalytic subunit.
Villa-Moruzzi
E
E
Ist. di Patologia Generale, Università di Pisa, Italy.
Crabb
J W
JW
D000818:Animals; D002845:Chromatography; D002850:Chromatography, Gel; D006003:Glycogen / Q000378:metabolism*; D046911:Macromolecular Substances; D008970:Molecular Weight; D009132:Muscles / Q000201:enzymology*; D010744:Phosphoric Monoester Hydrolases / Q000302:isolation & purification* / Q000378:metabolism; D011233:Precipitin Tests; D011817:Rabbits
D046911:Macromolecular Substances; D006003:Glycogen; D010744:Phosphoric Monoester Hydrolases