Conformation of two somatostatin analogues in aqueous solution. Study by NMR methods and circular dichroism.
Cyclic-disulfide-containing analogues of somatostatin, Xaa1-Cys2-Xaa3-DTrp4-Lys6-Thr5-Xaa7- Xaa8 [Xaa1 = H or DPhe; Xaa3 = Phe or Tyr; Xaa7 = Cys, Me2Cys or Me2DCys; Xaa8 = OH, Thr8 (OH) or Thr8NH2], were examined in aqueous solution by 1H-NMR spectroscopy and circular dichroism. The influence of the helical nature of the disulfide bridge and the presence of exocyclic residues on biological activity were investigated with particular care.
Wynants
C
C
Laboratory of Organic Chemistry, Vrije Universiteit Brussel, Belgium.
Tourwe
D
D
Kazmierski
W
W
Hruby
V J
VJ
Van Binst
G
G
D000595:Amino Acid Sequence; D002942:Circular Dichroism; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011487:Protein Conformation; D013004:Somatostatin / Q000031:analogs & derivatives*
D013004:Somatostatin