title: Mixed hematopoietic chimerism after allogeneic transplantation with lymphocyte-depleted bone marrow is not associated with a higher incidence of relapse.
abstract: Using red cell phenotyping, cytogenetic analysis of blood lymphocytes, chromosome studies of bone marrow cells, and restriction fragment length polymorphism (RFLP) studies of peripheral blood cells, we demonstrated a high number of mixed chimeras after allogeneic bone marrow transplantation (BMT). Donor marrow from HLA-A, -B, and -DR identical, mixed lymphocyte culture (MLC) nonreactive siblings was depleted of 98% of lymphocytes using counterflow centrifugation. Thirty-two of 48 recipients (67%) appeared to be mixed chimeras at 6 months after transplantation. The high number of mixed chimeras is probably a result of lymphocyte depletion of the marrow graft and the high sensitivity of red cell phenotyping for the demonstration of minor cell populations (at levels as low as 0.01%). The probability of relapse-free survival from 6 months to 4 years after BMT was 85% for the mixed chimeras and 65% for the complete donor chimeras. We conclude that in this study, mixed chimerism is not associated with a higher incidence of relapse.
authors.affiliation: Department of Internal Medicine, University Hospital, Nijmegen, The Netherlands.
authors.forename: A
authors.initials: A
authors.lastname: Schattenberg
authors.forename: T
authors.initials: T
authors.lastname: De Witte
authors.forename: M
authors.initials: M
authors.lastname: Salden
authors.forename: J
authors.initials: J
authors.lastname: Vet
authors.forename: B
authors.initials: B
authors.lastname: Van Dijk
authors.forename: D
authors.initials: D
authors.lastname: Smeets
authors.forename: J
authors.initials: J
authors.lastname: Hoogenhout
authors.forename: C
authors.initials: C
authors.lastname: Haanen
mesh_terms: D000293:Adolescent; D000328:Adult; D001789:Blood Group Antigens / Q000235:genetics; D001855:Bone Marrow Diseases / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D016026:Bone Marrow Transplantation; D002678:Chimera; D002871:Chromosome Banding; D005260:Female; D006412:Hematopoietic Stem Cells / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007621:Karyotyping; D008212:Lymphocyte Depletion; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D012150:Polymorphism, Restriction Fragment Length; D011184:Postoperative Period; D011300:Preoperative Care; D012008:Recurrence; D014019:Tissue Donors
chemical_list: D001789:Blood Group Antigens