High-resolution footprints of the DNA-binding domain of Epstein-Barr virus nuclear antigen 1.
The DNA-binding domain of Epstein-Barr virus nuclear antigen 1 was found by hydroxyl radical footprinting to protect backbone positions on one side of its DNA-binding site. The guanines contacted in the major groove by the DNA-binding domain of Epstein-Barr virus nuclear antigen 1 were identified by methylation protection. No difference was found in the interaction of the DNA-binding domain of Epstein-Barr virus nuclear antigen 1 with tandemly repeated and overlapping binding sites.
Kimball
A S
AS
Department of Biology, McCollum-Pratt Institute, Baltimore, Maryland.
Milman
G
G
Tullius
T D
TD
D000956:Antigens, Viral / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D004261:DNA Replication; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000276:immunology / Q000378:metabolism*; D019309:Epstein-Barr Virus Nuclear Antigens; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000276:immunology*; D008745:Methylation; D009690:Nucleic Acid Conformation; D009710:Nucleotide Mapping; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D014779:Virus Replication
D000956:Antigens, Viral; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D019309:Epstein-Barr Virus Nuclear Antigens