Production of discrete high specific activity DNA probes using the polymerase chain reaction.
Conditions are described for the synthesis of discrete DNA probes with high specific activity using the polymerase chain reaction. This method enables the production of DNA probes between any two oligonucleotide sequences from cloned or uncloned templates. These probes are uniform in length and their specific activity (1 x 10(9) cpm/microgram) is comparable to probes produced by other methods.
Jansen
R
R
Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030.
Ledley
F D
FD
D003570:Cytidine Triphosphate / Q000378:metabolism; D015342:DNA Probes; D004259:DNA-Directed DNA Polymerase; D005784:Gene Amplification; D005821:Genetic Techniques; D006801:Humans; D008765:Methylmalonyl-CoA Mutase / Q000235:genetics; D019914:Taq Polymerase; D013698:Templates, Genetic
D015342:DNA Probes; D003570:Cytidine Triphosphate; D019914:Taq Polymerase; D004259:DNA-Directed DNA Polymerase; D008765:Methylmalonyl-CoA Mutase