Leydig cell recovery following a second challenge with ethane-1,2-dimethanesulphonate is enhanced by short intervals between cytotoxin administration to rats.
Ethane-1,2-dimethanesulphonate (EDS) destroys Leydig cells in the testis of the adult rat and subsequently a new population of Leydig cells develops. It has been reported that EDS is not cytocidal to the new immature Leydig cell population. In the present study, the effect of increasing the time-interval between injections of EDS on cytotoxicity to Leydig cells was examined. At time-intervals of 4-10 weeks between injections the response was similar to that seen after a single injection of EDS to the adult rat. Four days after the second injection, EDS was found to reduce substantially serum testosterone concentrations and in-vitro binding of 125I-labelled human chorionic gonadotrophin (hCG) to testicular LH receptors which can be correlated with Leydig cell destruction. However, when the interval was only 2 or 3 weeks there was no reduction in serum testosterone, and 125I-labelled hCG binding was not so markedly reduced. During days 1-6 after a second injection of EDS, administered 3 weeks after the first, there were marked reductions in serum testosterone concentrations and in 125I-labelled hCG binding to testis homogenates within 24 h. Recovery from the effects of EDS was rapid, and increased Leydig cell activity was seen from 2 to 6 days after injection. In contrast to the established changes in the adult rat, there was only a 50% reduction in the number of Leydig cells positive for 3 beta-hydroxysteroid dehydrogenase 2 days after the second injection of EDS, and after 6 days the number of cells had increased.(ABSTRACT TRUNCATED AT 250 WORDS)
Edwards
G
G
Department of Physiological Sciences, University of Manchester.
Lendon
R
R
Morris
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ID
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D006063:Chorionic Gonadotropin / Q000378:metabolism; D004334:Drug Administration Schedule; D007985:Leydig Cells / Q000187:drug effects*; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D008698:Mesylates / Q000008:administration & dosage / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains; D013737:Testis / Q000378:metabolism; D013739:Testosterone / Q000097:blood
D006063:Chorionic Gonadotropin; D008698:Mesylates; D013739:Testosterone; D007986:Luteinizing Hormone; C002994:ethylene dimethanesulfonate