VIP-helodermin receptors in the murine virus-induced T lymphoma cell line BL/VL3 recover less rapidly than beta-adrenoceptors after down regulation.
The time course of recovery of beta-adrenergic and VIP/helodermin receptors after homologous and heterologous down regulation was studied in the murine lymphoma cell line BL/VL3, a neoplastic equivalent of immature T cells. The heterologous part of isoproterenol down regulation was rapidly reversed, even in the presence of cycloheximide, suggesting that down regulation was linked to ligand-receptor interaction and/or cyclic AMP increase. Homologous down regulations of beta-adrenoceptors and VIP/helodermin receptors were less rapidly reversible and depended on protein synthesis as they were inhibited by cycloheximide: beta-adrenoceptors recovered faster than VIP/helodermin receptors.
Damien
C
C
Department of Biochemistry and Nutrition, School of Medicine, Université Libre de Bruxelles, Belgium.
Robberecht
P
P
Abello
J
J
Hooghe
R
R
Christophe
J
J
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D015536:Down-Regulation / Q000502:physiology*; D036341:Intercellular Signaling Peptides and Proteins; D007545:Isoproterenol / Q000494:pharmacology; D008223:Lymphoma; D051379:Mice; D010455:Peptides / Q000494:pharmacology; D011943:Receptors, Adrenergic, beta / Q000502:physiology*; D011964:Receptors, Gastrointestinal Hormone / Q000502:physiology*; D018005:Receptors, Vasoactive Intestinal Peptide; D013601:T-Lymphocytes / Q000378:metabolism*; D014660:Vasoactive Intestinal Peptide
D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D011943:Receptors, Adrenergic, beta; D011964:Receptors, Gastrointestinal Hormone; D018005:Receptors, Vasoactive Intestinal Peptide; D014660:Vasoactive Intestinal Peptide; C040442:heliodermin; D000262:Adenylyl Cyclases; D007545:Isoproterenol