Determination of gold-labelled surface receptors on single cells by X-ray microanalysis.
A standardless X-ray microanalytical procedure has been developed to determine the number of gold-labelled surface receptors on whole single cells. The effect of the injection of K2PtCl4 into mice on gold-labelled concanavalin A (Con A) receptors on peritoneal macrophages was examined with an energy dispersive X-ray detector in an SEM. The numbers of gold particles seen in electron micrographs and estimated by fluorescence photometric measurements of fluorescein isothiocyanate-labelled Con A receptors were correlated with the X-ray microanalytical results.
Peschke
T
T
GDR Academy of Sciences, Central Institute of Microbiology and Experimental Therapy.
Augsten
K
K
Sasama
F
F
Gabert
A
A
D000818:Animals; D004577:Electron Probe Microanalysis / Q000379:methods*; D006046:Gold / Q000032:analysis*; D008264:Macrophages / Q000032:analysis; D008297:Male; D051379:Mice; D008855:Microscopy, Electron, Scanning; D011956:Receptors, Cell Surface / Q000032:analysis*; D011952:Receptors, Concanavalin A / Q000032:analysis
D011956:Receptors, Cell Surface; D011952:Receptors, Concanavalin A; D006046:Gold