Separation of human neutrophils in self-generated continuous density gradients of Percoll.
Human neutrophils were separated into two fractions using a continuous density gradient of Percoll solution. A marked decrease in O2- production was observed in the low-density neutrophil fraction. There was little erythrocyte contamination in the high-density neutrophil fraction (less than 0.5%), and thus the hypotonic or ammonium chloride lysis of erythrocytes was not necessary.
Uyesaka
N
N
Department of Physiology, Nippon Medical School, Tokyo, Japan.
Tajiri
A
A
Arai
K
K
D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D006801:Humans; D009504:Neutrophils / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D011205:Povidone; D012822:Silicon Dioxide
C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone; D010100:Oxygen