title: Vector electron paramagnetic resonance spectroscopy with first and second harmonic displays of ferrihemoglobin.
abstract: Superimposed plots of electron paramagnetic resonance spectra with the first and second harmonic displays of ferrihemoglobin at pH 9.1 and 90 K were measured at 20 degree intervals of phase angle using a phase-sensitive detector. The high spin signal in the g = 6 region was observed in both displays, and a small splitting of the signal was found in the calculated amplitude spectrum of the second harmonic display, with g values of 5.95 and 6.05. Low spin signals were observed at g = 2.55, 2.25 and 1.82 in both harmonic displays. A signal in the g = 2.05 region was observed only in the second harmonic display. The signal is probably associated with the low spin spectrum; however, its origin is obscure.
authors.affiliation: Department of Molecular Physiology, National Institute for Physiological Sciences, Okazaki, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Watari
authors.forename: M
authors.initials: M
authors.lastname: Murakami
authors.forename: Y
authors.initials: Y
authors.lastname: Seo
authors.forename: Y
authors.initials: Y
authors.lastname: Shimoyama
mesh_terms: D004578:Electron Spin Resonance Spectroscopy; D006863:Hydrogen-Ion Concentration; D008706:Methemoglobin
chemical_list: D008706:Methemoglobin