title: Peripheral blood stem cell autotransplantation in treatment of childhood cancer.
abstract: The levels of circulating hematopoietic progenitor cells were measured sequentially in eight children receiving chemotherapy for acute leukemia or neuroblastoma. Significant increases in the progenitor levels (up to 50-fold in CFU-GM numbers) were observed during post-chemotherapy cytopenia in all cases, but differences among individuals in the kinetics of recovery of less committed progenitors (CFU-mix) contrasted with the synchronized-mode of expansion of committed progenitors (CFU-GM). Peripheral blood cells were collected by repeated continuous-flow leukaphereses from three of the children during post-chemotherapy expansion of the progenitor pool and were cryopreserved after fractionation procedures. Infusion of these stored cells into the patients after marrow-ablative chemotherapy established trilineage hematopoiesis. This use of stem cell rescue should be useful as an alternative to bone marrow transplantation and extends the application of cure-oriented salvage therapy to childhood cancers.
authors.affiliation: Department of Pediatrics, University of Tokushima, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
authors.forename: Y
authors.initials: Y
authors.lastname: Takaue
authors.forename: Y
authors.initials: Y
authors.lastname: Kawano
authors.forename: T
authors.initials: T
authors.lastname: Koyama
authors.forename: M A
authors.initials: MA
authors.lastname: Huq
authors.forename: T
authors.initials: T
authors.lastname: Shimokawa
authors.forename: T
authors.initials: T
authors.lastname: Ninomiya
authors.forename: Y
authors.initials: Y
authors.lastname: Aga
authors.forename: T
authors.initials: T
authors.lastname: Inai
authors.forename: M
authors.initials: M
authors.lastname: Hino
mesh_terms: D001772:Blood Cell Count; D002648:Child; D002675:Child, Preschool; D003114:Colony-Forming Units Assay; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007223:Infant; D007700:Kinetics; D015470:Leukemia, Myeloid, Acute / Q000097:blood / Q000628:therapy; D008228:Lymphoma, Non-Hodgkin / Q000097:blood / Q000628:therapy; D009369:Neoplasms / Q000097:blood / Q000628:therapy*; D009447:Neuroblastoma / Q000097:blood / Q000628:therapy; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000097:blood / Q000628:therapy; D014182:Transplantation, Autologous