Evaluation of the use of S1 nuclease to detect small length variations in genomic DNA.
A method which utilises S1 nuclease to detect small length variations in cloned and genomic DNA has been evaluated. The methodology of this technique is simple and robust, permitting the rapid analysis of 10(4) base pairs. By employing defined sequence variants, this method is shown to have a sensitivity which should enable the detection of length variations of only a few base pairs in heterozygous individuals.
Brookes
A J
AJ
Department of Somatic Cell Genetics, Imperial Cancer Research Fund, London, UK.
Solomon
E
E
D001482:Base Composition; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000378:metabolism; D015342:DNA Probes; D004262:DNA Restriction Enzymes; D004720:Endonucleases / Q000378:metabolism*; D014644:Genetic Variation; D006579:Heterozygote; D006801:Humans; D009692:Nucleic Acid Heteroduplexes; D009693:Nucleic Acid Hybridization; D010013:Osteogenesis Imperfecta / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D015719:Single-Strand Specific DNA and RNA Endonucleases
D015342:DNA Probes; D009692:Nucleic Acid Heteroduplexes; D004247:DNA; D004720:Endonucleases; D004262:DNA Restriction Enzymes; D015719:Single-Strand Specific DNA and RNA Endonucleases