Electronic interaction chromatography on porous graphitic carbon. Separation of [99mTc]pertechnetate and perrhenate anions.
The oxo anions of technetium-99m and rhenium, pertechnetate (TcO4-) and perrhenate (ReO4-), have been separated by high performance liquid chromatography on porous graphitic carbon with aqueous trifluoroacetic acid or salt solutions as eluent. Chromatographic retention was a result of electronic interaction between the lone pair electrons of the anions and the delocalised pi-electron clouds of the porous graphitic carbon. Retention and resolution can be controlled by the concentration and/or species of eluents which can compete with the solutes for electronic interaction being used for elution.
Lim
C K
CK
Division of Clinical Cell Biology, MRC Clinical Research Centre, Middlesex, UK.
D000838:Anions; D002244:Carbon; D002853:Chromatography, Liquid / Q000379:methods*; D011874:Radiometry; D012211:Rhenium / Q000302:isolation & purification*; D013670:Sodium Pertechnetate Tc 99m / Q000302:isolation & purification*
D000838:Anions; C015428:perrhenate; D012211:Rhenium; D002244:Carbon; D013670:Sodium Pertechnetate Tc 99m