title: Light-induced DNA cleavage by esperamicin and neocarzinostatin.
abstract: Ultraviolet radiation of the enediyne drugs is effective in causing nicks in supercoiled DNA. Of special interest is the fact that the observed nucleotide cleaving specificity for the UV light- and thiol-activated antibiotics was the same with esperamicin A1, but was different with neocarzinostatin. In addition to the preferred cutting of T and A bases, the light-activated neocarzinostatin attacked certain G bases which were rarely cleaved by the thiol-activated neocarzinostatin. It should be noted that these enediyne antibiotics lose the DNA breakage activity after light-exposure for 30 min.
authors.affiliation: Institute for Chemical Research, Kyoto University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Uesawa
authors.forename: J
authors.initials: J
authors.lastname: Kuwahara
authors.forename: Y
authors.initials: Y
authors.lastname: Sugiura
mesh_terms: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D001483:Base Sequence; D004247:DNA / Q000187:drug effects* / Q000528:radiation effects; D004249:DNA Damage; D004578:Electron Spin Resonance Spectroscopy; D053281:Enediynes; D004926:Escherichia coli; D008969:Molecular Sequence Data; D010957:Plasmids / Q000187:drug effects* / Q000528:radiation effects; D014466:Ultraviolet Rays; D009353:Zinostatin / Q000494:pharmacology*
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; D053281:Enediynes; D004247:DNA; D009353:Zinostatin; C047543:esperamicin A1