Cloning and characterization of the SKI3 gene of Saccharomyces cerevisiae demonstrates allelism to SKI5.
We have identified a mutant strain of the yeast Saccharomyces cerevisiae which overproduces killer toxin. This strain contains a single mutation which fails to complement defects in both the SKI3 and SKI5 genes, while a cloned copy of this gene complements both the ski3 and ski5 defects. The level of secreted toxin from a cDNA based plasmid is not increased in a ski3 strain, showing that the overproduction phenotype is dependent upon an increased level of M1 dsRNA.
Hougan
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L
Genetic Engineering Section, NRC Biotechnology Research Institute, Montreal, Quebec, Canada.
Thomas
D Y
DY
Whiteway
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M
D000483:Alleles; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D015966:Gene Expression Regulation, Fungal / Q000235:genetics; D005816:Genetic Complementation Test; D055646:Killer Factors, Yeast; D009154:Mutation; D009183:Mycotoxins / Q000096:biosynthesis / Q000235:genetics*; D012330:RNA, Double-Stranded / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins
D004251:DNA Transposable Elements; D055646:Killer Factors, Yeast; D009183:Mycotoxins; D012330:RNA, Double-Stranded; D029701:Saccharomyces cerevisiae Proteins