Interleukin-2- and phytohemagglutinin-activated proliferation of human T-lymphocytes is accompanied by stimulation of phosphoinositide turnover.
Interleukin-2 (IL-2) was more effective than phytohemagglutinin (PHA) in increasing the proliferative activity of human T-lymphocytes. Unlike PHA, IL-2 stimulated phosphoinositide turnover (PI turnover) only in those T-lymphocytes which had been preactivated by PHA with IL-2 and expressed the IL-2 receptors. The effect of PHA on PI turnover was, in general, stronger than that of IL-2. These results indicate that IL-2 and PHA-activated proliferation of human T-lymphocytes is accompanied by stimulation of PI turnover. However, IL-2-induced proliferative response may not be a direct consequence of PI turnover stimulation in these cells.
Korichneva
I L
IL
Institute of Experimental Cardiology, National Cardiology Research Center, Moscow, U.S.S.R.
Grigorian GYu
Krasnikova
T L
TL
Rudchenko
S A
SA
Tkachuk
V A
VA
D002455:Cell Division; D004247:DNA / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D007399:Interphase; D007700:Kinetics; D010716:Phosphatidylinositols / Q000378:metabolism*; D010835:Phytohemagglutinins / Q000494:pharmacology*; D015375:Receptors, Interleukin-2 / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology* / Q000378:metabolism
D007376:Interleukin-2; D010716:Phosphatidylinositols; D010835:Phytohemagglutinins; D015375:Receptors, Interleukin-2; D004247:DNA