Characterization of a parvovirus isolated from a pig fetus.
A small hemagglutinating virus belonging to the Parvoviridae Family was isolated from a 70 day-old pig fetus in a breeding herd in which infertility, metritis and abortions were reported. The virus, named 85/193L, was isolated either in primary pig kidney (PK) cells or in a continuous cell line of minipig kidney origin (MPK), both cell cultures actively growing. It produced a typical cytopathic effect (CPE) starting from the 3rd passage and intranuclear inclusions surrounded by a halo were observed in stained preparations. The isolate was completely resistant to ether, chloroform and to pH 3; it was not inactivated after treatment at 56 degrees C for 4 h and at 70 degrees C for 2 h, while it was completely inhibited by the 80 degrees C/30 min temperature. It contained deoxyribonucleic acid (DNA). The highest infectious titer was reached at 96 h post infection. The infectivity and the hemagglutinating activity of the isolated strain were both inhibited by the reference immune serum against NADL-2 pig parvovirus. This further confirmed that the 85/193L isolate belongs to the parvovirus genus.
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Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia, Brescia, Italy.
Gualandi
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Capucci
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D000034:Abortion, Veterinary / Q000382:microbiology*; D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D005260:Female; D005313:Fetal Death / Q000382:microbiology / Q000662:veterinary*; D006385:Hemagglutination Inhibition Tests; D009500:Neutralization Tests; D010321:Parvoviridae / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification*; D010322:Parvoviridae Infections / Q000382:microbiology / Q000662:veterinary*; D011247:Pregnancy; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*