[Protein composition of DNA-matrix complexes with "strong" and "weak" types of bonds, isolated from Ehrlich ascites carcinoma cells].
A preparative procedure for obtaining tightly and loosely bound DNA-matrix complexes was developed. To this end, the DNA-matrix complexes were separated from DNA excess by restrictase digestion and solubilized in 0.1% sodium dodecyl sulfate + 8 M urea. To obtain tightly bound complexes, the lysate was transferred to a solution containing 2 M LiCl and 4 mM urea and gel filtered in the same medium. Tightly bound complexes were eluted in the same peak with DNA. Loosely bound complexes were obtained by gel filtration in 0.1% sodium dodecyl sulfate + 8 M urea after preliminary digestion of the original complexes with DNAase I which selectively destroys the tight bonds. Within the composition of loosely bound DNA-matrix complexes, the polypeptides with Mr of 180, 65-75, 58 and 47-50 kDa were identified, whereas tightly bound complexes were shown to contain polypeptides with molecular masses of 180, 65-75, 63, 61, 58 and 52-53 kDa.
S'iakste
N I
NI
Blokhin
D Iu
DIu
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D002850:Chromatography, Gel; D004262:DNA Restriction Enzymes; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D014407:Tumor Cells, Cultured / Q000378:metabolism
D004268:DNA-Binding Proteins; D004262:DNA Restriction Enzymes
Belkovyĭ sostav kompleksov DNK-matriks s "prochnym" i "slabym" tipom sviazi, vydelennykh iz kletok astsitnoĭ kartsinomy érlikha.