Antibody response of pseudorabies virus subunit-vaccinated pigs to viral nucleocapsid proteins following low-dose virus challenge of immunity.
The antibody response to pseudorabies virus nucleocapsid proteins (NCP) was evaluated by the western immunoblot analysis before and after challenge of immunity by nasal inoculation of 10(2.3) plaque-forming units of virus in 10 pigs that had been vaccinated with pseudorabies virus envelope glycoproteins. Antibody to 5 NCP with molecular mass of 140, 63, 41, 34, and 23 kD was first detected in vaccinated and nonvaccinated pigs on day 14 after challenge of immunity. Antibody to 2 of the 5 NCP continued to be detected through day 113 in 9 of 10 vaccinated pigs. Beyond day 32, antibody to NCP was not detected in 1 vaccinated pig. The 23-, 34-, and 41-kD proteins were the most immunogenic. Antibody to each of these proteins was first detected on day 14 in 10, 10, and 8 pigs, respectively. Seven, 6, and 8 pigs, respectively, were antibody-positive for these proteins on day 113. The 140- and 63-kD proteins were the least immunogenic. Antibody to these proteins was detected in 8 and 9 pigs, respectively, on day 14, and in 4 and 5 pigs, respectively, on day 113. Chi-square analysis for dependency indicated that the antibody response to the 140- and 63-kD proteins was interdependent. These results suggested that combinations of NCP may be useful as nonvaccine diagnostic antigens.
McGinley
M J
MJ
Department of Veterinary Microbiology, Iowa State University, Ames 50011.
Platt
K B
KB
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D015153:Blotting, Western; D002213:Capsid / Q000276:immunology; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D008970:Molecular Weight; D011557:Pseudorabies / Q000276:immunology*; D013552:Swine; D013553:Swine Diseases / Q000276:immunology*; D014611:Vaccination / Q000662:veterinary; D014758:Viral Core Proteins / Q000276:immunology; D014765:Viral Vaccines / Q000032:analysis / Q000276:immunology*
D000914:Antibodies, Viral; D014758:Viral Core Proteins; D014765:Viral Vaccines