Restriction endonuclease analysis of four Borrelia burgdorferi strains.
A restriction endonuclease analysis was performed on four strains of Borrelia burgdorferi: one isolated from man (SF), one from Ixodes dammini (B31) and two from I. ricinus (BITS in Italy and B45 in Germany). Digestion by Taq I and Hae III gave the best resolution of the DNA fragments. Three different restriction patterns were obtained: BITS and B45 showed only one band difference. These results correlate with the reactivity of the four strains with monoclonal antibodies.
Cinco
M
M
Istituto di Microbiologia, Università di Trieste, Italy.
Banfi
E
E
Balanzin
D
D
Cacciò
S
S
Graziosi
G
G
Fattorini
P
P
D000911:Antibodies, Monoclonal; D015748:Borrelia burgdorferi Group / Q000235:genetics* / Q000276:immunology; D004262:DNA Restriction Enzymes / Q000494:pharmacology; D004269:DNA, Bacterial / Q000032:analysis*
D000911:Antibodies, Monoclonal; D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes