Bacteriophage association of streptococcal pyrogenic exotoxin type C.
A gene encoding streptococcal pyrogenic exotoxin type C (SPE C) was isolated from bacteriophage DNA derived from Streptococcus pyogenes CS112. The gene, designated speC2, was shown to reside near the phage attachment site of phage CS112. A restriction endonuclease map of the CS112 phage was generated, and the location and orientation of the speC2 gene were determined. Hybridization analyses of eight SPE C-producing strains revealed restriction fragment length polymorphism of the speC gene-containing DNA fragments and further showed that each speC was linked to a common CS112 phage-derived DNA fragment.
Goshorn
S C
SC
Department of Microbiology, Medical School, University of Minnesota, Minneapolis 55455.
Schlievert
P M
PM
D001427:Bacterial Toxins / Q000235:genetics*; D001435:Bacteriophages / Q000235:genetics*; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D005098:Exotoxins / Q000235:genetics*; D005796:Genes; D005814:Genes, Viral; D005779:Immunodiffusion; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D013297:Streptococcus pyogenes / Q000235:genetics*
D001427:Bacterial Toxins; D004279:DNA, Viral; D005098:Exotoxins