Effects of recombinant interleukin-1 beta on phospholipase A2 activity, phospholipase A2 mRNA levels, and eicosanoid formation in rabbit chondrocytes.
We have investigated the effects of recombinant interleukin-1 beta (rIL-1 beta) on phospholipase A2 activity (PLA2), PLA2 messenger RNA levels, and eicosanoid production in rabbit chondrocytes. Phospholipase A2 activity increased 5 fold with exposure to 1.6 x 10(-11) M rIL-1 beta for 20 hours. An mRNA specific for an extracellular PLA2 was detected by RNA blot hybridization after treatment of the cells with rIL-1 beta. Hydroxylated derivatives of arachidonic acid, including prostaglandins, leukotriene B4, 5-hydroxyeicosa 6E, 8Z, 11Z, 14Z-tetraenoic acid, 12-hydroxyeicosa 5Z, 8Z, 10E, 14Z-tetraenoic acid and 15-hydroxyeicosa 5Z, 8Z, 11Z, 13E-tetraenoic acid increased in cells after rIL-1 beta treatment.
Kerr
J S
JS
Medical Products Department, E. I. Du Pont de Nemours & Co. (Inc.), Wilmington, DE 19880-0400.
Stevens
T M
TM
Davis
G L
GL
McLaughlin
J A
JA
Harris
R R
RR
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism; D002358:Cartilage, Articular / Q000201:enzymology*; D015342:DNA Probes; D015237:Dinoprost / Q000096:biosynthesis; D015232:Dinoprostone / Q000096:biosynthesis; D015777:Eicosanoids / Q000096:biosynthesis*; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis; D007375:Interleukin-1 / Q000494:pharmacology*; D007700:Kinetics; D007975:Leukotriene B4 / Q000096:biosynthesis; D008297:Male; D009693:Nucleic Acid Hybridization; D010740:Phospholipases / Q000378:metabolism*; D010741:Phospholipases A / Q000235:genetics / Q000378:metabolism*; D054467:Phospholipases A2; D015230:Prostaglandin D2 / Q000096:biosynthesis; D012333:RNA, Messenger / Q000032:analysis*; D011817:Rabbits; D011994:Recombinant Proteins
D001095:Arachidonic Acids; D015342:DNA Probes; D015777:Eicosanoids; D006893:Hydroxyeicosatetraenoic Acids; D007375:Interleukin-1; D012333:RNA, Messenger; D011994:Recombinant Proteins; D007975:Leukotriene B4; D015237:Dinoprost; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D015232:Dinoprostone; D015230:Prostaglandin D2