Thyroid hormone effect on rat heart mitochondrial proteins and affinity labeling with N-bromoacetyl-3,3',5-triiodo-L-thyronine. Lack of direct effect on the adenine nucleotide translocase.
N-bromoacetyl-3,3',5-tri[3'-125I]iodo-L-thyronine was used to label intact heart mitochondria from eu, hypo- and hyperthyroid rats in order to identify proteins involved in T3-regulated mitochondrial processes. The results show strong labeling, competed for by T3 and other analogues, of two proteins with a molecular mass of 48,000 and 49,200 Da. No labeling is seen of the adenine nucleotide translocase, a likely target, neither at 0 degree C, at room temperature, nor after preincubation with the substrates or specific inhibitors. No difference in labeling intensity or distribution is seen in mitochondria from eu-, hypo- or hyperthyroid rats, and the abundance of the adenine nucleotide translocase is unchanged, but five other proteins show differential abundance.
Rasmussen
U B
UB
Abteilung Endokrinologie, Medizinische Hochschule Hannover, FRG.
Köhrle
J
J
Rokos
H
H
Hesch
R D
RD
D000345:Affinity Labels / Q000378:metabolism*; D000818:Animals; D006980:Hyperthyroidism / Q000378:metabolism*; D007037:Hypothyroidism / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D008929:Mitochondria, Heart / Q000378:metabolism*; D000226:Mitochondrial ADP, ATP Translocases / Q000378:metabolism*; D009713:Nucleotidyltransferases / Q000378:metabolism*; D011441:Propylthiouracil / Q000494:pharmacology; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D011917:Rats, Inbred Lew; D012016:Reference Values; D013961:Thyroid Gland / Q000502:physiology*; D013974:Thyroxine / Q000097:blood / Q000494:pharmacology; D014284:Triiodothyronine / Q000097:blood / Q000378:metabolism / Q000031:analogs & derivatives
D000345:Affinity Labels; D011506:Proteins; D014284:Triiodothyronine; D011441:Propylthiouracil; C036219:N-bromoacetyl-3,3',5-triiodothyronine; D000226:Mitochondrial ADP, ATP Translocases; D009713:Nucleotidyltransferases; D013974:Thyroxine