title: Purification and characterization of cytolysins from Listeria monocytogenes serovar 4b and Listeria ivanovii.
abstract: Several exoproteins from Listeria monocytogenes serovar 4b (NCTC 10527) and Listeria ivanovii (ATCC) 19119, SLCC 2379), respectively, have been purified to homogeneity by thiol-disulfide exchange chromatography and gel filtration. Both strains produce a haemolytic/cytolytic protein of Mr 58 kDa, which has all the properties of a SH-activated cytolysin, the prototype of which is streptolysin O (SLO), and this protein has therefore been termed listeriolysin O (LLO). In addition a protein of Mr 24 kDa from culture supernatants of L. ivanovii co-purified with LLO. The N-terminal aminoacid sequences of both proteins from L. ivanovii have been determined. By mutagenesis with transposons of Gram-positive origin (Tn916 and Tn1545), which have been introduced via conjugation into L. ivanovii, several phenotypic mutants (altered haemolysis on sheep blood agar or lecithinase-negative) were obtained. Results on the properties of these mutants will be presented.
authors.affiliation: Institute of Genetics and Microbiology, University of Würzburg, FRG.
authors.forename: J
authors.initials: J
authors.lastname: Kreft
authors.forename: D
authors.initials: D
authors.lastname: Funke
authors.forename: R
authors.initials: R
authors.lastname: Schlesinger
authors.forename: F
authors.initials: F
authors.lastname: Lottspeich
authors.forename: W
authors.initials: W
authors.lastname: Goebel
mesh_terms: D000595:Amino Acid Sequence; D001427:Bacterial Toxins; D004251:DNA Transposable Elements; D006360:Heat-Shock Proteins / Q000032:analysis* / Q000235:genetics; D006460:Hemolysin Proteins / Q000032:analysis* / Q000235:genetics; D008087:Listeria / Q000032:analysis* / Q000235:genetics; D008969:Molecular Sequence Data; D010641:Phenotype; D010740:Phospholipases / Q000032:analysis; D013108:Sphingomyelin Phosphodiesterase / Q000032:analysis
chemical_list: D001427:Bacterial Toxins; D004251:DNA Transposable Elements; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D010740:Phospholipases; D013108:Sphingomyelin Phosphodiesterase; C038705:hlyA protein, Listeria monocytogenes