title: Ontogeny of glutamate accumulating activity in rat brain synaptic vesicles.
abstract: The relationship between the ontogeny of the vesicular glutamate uptake system and synaptogenesis in rats was investigated. For this purpose we have developed a simplified procedure for the preparation of crude synaptic vesicles which are sufficiently pure to demonstrate a highly ATP-dependent glutamate uptake. ATP-dependent glutamate uptake into synaptic vesicles was found to increase dramatically starting on postnatal day 10 and reaching a maximum on day 30 (76 +/- 40 and 657 +/- 40 pmol/mg protein/10 min, respectively), correlating well with the active period of synaptogenesis. Stimulation of uptake by chloride also developed in parallel with the vesicular glutamate uptake. In contrast, combined non-ATP-dependent uptake and non-specific binding remained constant (21 +/- 6 pmol/mg protein/10 min). This development of vesicular glutamate uptake during the period of synaptogenesis supports the notion that synaptic vesicles play an important role in glutamate synaptic transmission.
authors.affiliation: Mental Health Research Institute, University of Michigan, Ann Arbor 48109.
authors.forename: P E
authors.initials: PE
authors.lastname: Kish
authors.forename: S Y
authors.initials: SY
authors.lastname: Kim
authors.forename: T
authors.initials: T
authors.lastname: Ueda
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000254:growth & development / Q000378:metabolism*; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D005971:Glutamates / Q000493:pharmacokinetics*; D018698:Glutamic Acid; D008565:Membrane Proteins / Q000378:metabolism*; D051381:Rats; D013347:Subcellular Fractions / Q000378:metabolism; D013572:Synaptic Vesicles / Q000378:metabolism* / Q000502:physiology
chemical_list: D005971:Glutamates; D008565:Membrane Proteins; D018698:Glutamic Acid; D017301:Ca(2+) Mg(2+)-ATPase