Serological typing scheme for BK-like isolates of human polyomavirus.
Human polyomavirus BK-like isolates were subjected to restriction endonuclease analysis with the enzymes EcoRI and Hind III. End-point dilution was used to obtain homogeneous virus pools for DNA analysis and to remove JC virus from a mixed stock. The results of Hind III digestion suggested that two subgroups could be distinguished. Several BK-like isolates were purified and rabbit antisera raised. The isolates were compared with each other and with BK and JC viruses by haemagglutination-inhibition (HI) and by neutralisation. JC virus was serologically distinct, but all the other isolates showed some cross-reactivity. Two subgroups were again evident: GS and PG were with prototype BK in subgroup 1, and MG and IV were in subgroup 2. Two isolates, AS and SB, reacted with isolates of both subgroups 1 and 2 but were distinct from one another: their genome was similar to subgroup 1 isolates. Typing by HI or by neutralisation may form a basis for grouping BK-like polyomavirus isolates.
Knowles
W A
WA
Virus Reference Laboratory, Central Public Health Laboratory, Colindale, London, England.
Gibson
P E
PE
Gardner
S D
SD
D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000276:immunology; D001739:BK Virus / Q000145:classification / Q000276:immunology / Q000302:isolation & purification; D002478:Cells, Cultured; D004279:DNA, Viral / Q000032:analysis* / Q000302:isolation & purification; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; D005260:Female; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007153:Immunologic Deficiency Syndromes / Q000235:genetics / Q000382:microbiology; D007577:JC Virus / Q000145:classification / Q000276:immunology / Q000302:isolation & purification; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D009500:Neutralization Tests; D011120:Polyomavirus / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification; D015183:Restriction Mapping; D012703:Serotyping
D000914:Antibodies, Viral; D004279:DNA, Viral; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII