Site-specific recombinations between direct and inverted res sites of Tn2501.
The resolvase gene and the putative res site of Tn2501 are not closely related to any of the previously described resolution functions. In view of this divergence, we designed genetic experiments to confirm the localization of the res site. We analyzed the activity of the Tn2501-encoded resolvase on substrates containing either directly or invertedly repeated res sites. These experiments confirm the localization of the res site that was predicted from nucleotide sequence data and show that the Tn2501 resolvase promotes site-specific inversions in vivo.
Michiels
T
T
Université Catholique de Louvain, Unité de Microbiologie, UCL 54-90, Brussels, Belgium.
Cornelis
G
G
D001483:Base Sequence; D002701:Chloramphenicol / Q000494:pharmacology; D002872:Chromosome Deletion; D007446:Chromosome Inversion; D004251:DNA Transposable Elements; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D011995:Recombination, Genetic; D015183:Restriction Mapping
D004251:DNA Transposable Elements; D002701:Chloramphenicol