Production of interleukin 1-like factor from human peripheral blood monocytes and polymorphonuclear leukocytes by superoxide anion: the role of interleukin 1 and reactive oxygen species in inflamed sites.
In the present study, we investigated the possibility that oxidative stress to human peripheral blood monocytes and polymorphonuclear leukocytes (PMNs) can induce interleukin 1 (IL-1)-like activity. Oxidative stress that we used was superoxide anion (O2-) and hydrogen peroxide (H2O2). O2-, but not H2O2, could induce an IL-1-like factor(s) from monocytes and PMNs. IL-1-like activity from monocytes and PMNs induced by O2- was due to de novo synthesis because no IL-1-like activity was found in culture supernatants and in the lysate of unstimulated cells. We next examined the effects of radical scavengers on production of an IL-1-like factor(s). Generation of IL-1-like activity from monocytes was amplified by preincubation with catalase (H2O2 scavenger), although it was suppressed by preincubation with either superoxide dismutase (O2- scavenger) or vitamin E (antioxidant analogs). These results suggest that production of an IL-1-like factor(s) from monocytes and PMNs was due to O2- stimulation. Our data that production of an IL-1-like factor(s) from inflammatory cells by stimulation with O2- imply a model of the up-regulation mechanism of inflammation mediated by enhanced IL-1-like factor production stimulated with reactive oxygen species.
Kasama
T
T
First Department of Internal Medicine, Showa University School of Medicine, Tokyo, Japan.
Kobayashi
K
K
Fukushima
T
T
Tabata
M
M
Ohno
I
I
Negishi
M
M
Ide
H
H
Takahashi
T
T
Niwa
Y
Y
D005949:Glucose Oxidase / Q000502:physiology; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D066298:In Vitro Techniques; D007249:Inflammation / Q000503:physiopathology*; D007375:Interleukin-1 / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000032:analysis; D009000:Monocytes / Q000502:physiology*; D009504:Neutrophils / Q000502:physiology*; D010084:Oxidation-Reduction; D010587:Phagocytosis; D013481:Superoxides / Q000378:metabolism*; D014969:Xanthine Oxidase / Q000502:physiology
D007375:Interleukin-1; D007376:Interleukin-2; D013481:Superoxides; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D014969:Xanthine Oxidase