title: Thyroid hormone stimulates expression of 6-phosphofructo-2-kinase in rat liver.
abstract: The activity of liver 6-phosphofructo-2-kinase (PFK-2), the enzyme that catalyses the synthesis of fructose 2,6-bisphosphate, was markedly decreased in hypothyroid rats and partially restored after 3 days of treatment with triiodothyronine. The changes in PFK-2 activity were accompanied by parallel changes in enzyme content measured by immunotitration and in PFK-2 mRNA determined by dot blot and Northern blot hybridization with cDNA probes. It is concluded that thyroid hormone stimulates liver PFK-2 gene expression by a pre-translational mechanism.
authors.affiliation: Hormone and Metabolic Research Unit, International Institute of Cellular and Molecular Pathology, Brussels, Belgium.
authors.forename: S R
authors.initials: SR
authors.lastname: Wall
authors.forename: M F
authors.initials: MF
authors.lastname: van den Hove
authors.forename: K M
authors.initials: KM
authors.lastname: Crepin
authors.forename: L
authors.initials: L
authors.lastname: Hue
authors.forename: G G
authors.initials: GG
authors.lastname: Rousseau
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D015342:DNA Probes; D005635:Fructosediphosphates / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D007037:Hypothyroidism / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008297:Male; D009206:Myocardium / Q000201:enzymology; D025481:Phosphofructokinase-2; D010770:Phosphotransferases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D013963:Thyroid Hormones / Q000494:pharmacology*; D014284:Triiodothyronine / Q000494:pharmacology
chemical_list: D015342:DNA Probes; D005635:Fructosediphosphates; D012333:RNA, Messenger; D013963:Thyroid Hormones; D014284:Triiodothyronine; D010770:Phosphotransferases; D025481:Phosphofructokinase-2