Analysis of B-G and immune response genes in the Iowa State University S1 chicken line by hybridization of sperm deoxyribonucleic acid with a major histocompatibility complex class II probe.
Sperm DNA was isolated from chickens of the Iowa State University S1 line. Birds were from sublines selected for B-G antigen, humoral immune response to glutamic acid-alanine-tyrosine (IrGAT), and response to Rous sarcoma virus-induced (RSV) tumors. The DNA was digested with restriction enzymes and subjected to Southern blot analysis with a DNA probe specific for a class II gene of the chicken major histocompatibility complex (MHC). Restriction fragment length polymorphisms (RFLP) associated with B-G antigen type were found after digestion of DNA with three enzymes, PvuII, BglII, or Sau3A, out of a total of 15 tested. No RFLP were shown to be associated with IrGAT or RSV type. This study shows that RFLP analysis of DNA may be a useful addition to or alternative to serological evaluation of MHC haplotype in the chicken.
Pitcovski
J
J
Department of Biochemistry and Biophysics, Iowa State University, Ames 50011.
Lamont
S J
SJ
Nordskog
A W
AW
Warner
C M
CM
D000818:Animals; D015139:Blotting, Southern; D002645:Chickens / Q000235:genetics* / Q000276:immunology*; D004247:DNA / Q000032:analysis*; D005802:Genes, MHC Class II; D008285:Major Histocompatibility Complex; D008297:Male; D009693:Nucleic Acid Hybridization; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D013094:Spermatozoa / Q000032:analysis*
D004247:DNA