Specific binding of okadaic acid, a new tumor promoter in mouse skin.
The tumor promoter okadaic acid binds specifically to a particulate as well as a cytosolic fraction of various mouse tissues, e.g., skin, brain, lung and colon. The KD value was 21.7 nM for receptors in the particulate fraction and 1.0 nM for those in the cytosolic fraction of mouse skin. The specific binding of [3H]okadaic acid to the particulate fraction of mouse skin was inhibited dose-dependently by okadaic acid, but not okadaic acid tetramethyl ether, an inactive compound, or by other tumor promoters, such as 12-O-tetradecanoylphorbol-13-acetate and teleocidin. The results suggest a new pathway of tumor promotion mediated through the okadaic acid receptor(s).
Suganuma
M
M
Cancer Prevention Division, National Cancer Center Research Institute, Tokyo, Japan.
Suttajit
M
M
Suguri
H
H
Ojika
M
M
Yamada
K
K
Fujiki
H
H
D000818:Animals; D001921:Brain / Q000378:metabolism; D002273:Carcinogens / Q000378:metabolism*; D002848:Chromatography, DEAE-Cellulose; D003106:Colon / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004988:Ethers, Cyclic / Q000378:metabolism*; D008168:Lung / Q000378:metabolism; D051379:Mice; D019319:Okadaic Acid; D010749:Phosphoprotein Phosphatases / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism; D012867:Skin / Q000201:enzymology / Q000378:metabolism*
D002273:Carcinogens; D004988:Ethers, Cyclic; D019319:Okadaic Acid; D011494:Protein Kinases; D010749:Phosphoprotein Phosphatases