Origin of rare Ha-ras alleles: relationship of VTR length to a 5' polymorphic Xho I site.
Amongst the four common Ha-ras alleles in both controls and cancer patients, we detected the presence of a polymorphic Xho I site associated specifically with the 6.6 and 7.7 kb Bam HI fragments but absent from the 7.1 and 8.2 kb alleles, as recently reported by others. We have extended this study and report here, the consistent appearance of this Xho I site in unusual alleles close in size to the two common alleles of 6.6 and 7.7 kb, in control lymphoblastoid DNA samples in a variety of tumor DNAs. Unusual alleles grouped around the 7.1 and 8.2 kb common alleles on the other hand, did not possess the Xho I site. The consistent presence of the Xho I site polymorphism, in the unusual Ha-ras alleles surrounding the 6.6 and 7.7 kb common alleles and its absence in alleles around the 7.1 and 8.2 kb common alleles, suggests that the unusual ones are derived from the corresponding common alleles to which they are closest in size.
Baxter
G D
GD
Hayward
N K
NK
Collins
R J
RJ
Lavin
M F
MF
D000483:Alleles; D004273:DNA, Neoplasm / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D011905:Genes, ras; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics; D007938:Leukemia / Q000235:genetics; D008545:Melanoma / Q000235:genetics; D009369:Neoplasms / Q000235:genetics*; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D009396:Wilms Tumor / Q000235:genetics
D004273:DNA, Neoplasm; C034510:CTCGAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific