Superoxide--driven oxidation of quercetin and a simple sensitive assay for determination of superoxide dismutase.
Oxidation of quercetin at pH 10 was shown to be a free radical chain reaction involving superoxide and hence inhibitable by superoxide dismutase (SOD) (EC 1.15.1.1). The degree of inhibition of quercetin oxidation was a function of SOD concentration, and fifty percent inhibition was produced by approximately 1.5 ng/ml of pure enzyme. This reaction proved to be a very useful tool for a rapid and highly sensitive measurement of SOD in crude tissue extracts and other biological samples.
Kostyuk
V A
VA
Department of Biology, V. I. Lenin Byelorussion State University, Minsk, BSSR, USSR.
Potapovich
A I
AI
D000818:Animals; D001769:Blood / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D005260:Female; D005419:Flavonoids / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000378:metabolism; D008297:Male; D011794:Quercetin / Q000378:metabolism*; D051381:Rats; D013482:Superoxide Dismutase / Q000032:analysis*; D013481:Superoxides / Q000378:metabolism*; D013582:Synovial Fluid / Q000378:metabolism; D013997:Time Factors
D005419:Flavonoids; D013481:Superoxides; D011794:Quercetin; D013482:Superoxide Dismutase