title: Immune response to immunostimulatory complexes (ISCOMs) prepared from human immunodeficiency virus type 1 (HIV-1) or the HIV-1 external envelope glycoprotein (gp120).
abstract: In mice, immunostimulatory complexes (ISCOMs) prepared from HIV-1 B external envelope glycoprotein (gp120) induced 10-fold higher antibody titres than gp120 emulsified in depot adjuvant, as measured by enzyme-linked immunosorbent assay (ELISA). Rhesus monkeys immunized with gp120 ISCOMs produced precipitating and virus neutralizing antibody titres equivalent to those seen in HIV-infected chimpanzees and humans. After multiple immunizations with HIV-1 B gp120 ISCOMs, a rhesus monkey developed a neutralizing response to the HIV-1 isolates RF and MN, but not to the CC isolate. Antisera from ISCOM-immunized rhesus monkeys recognized gp120 on the membranes of HIV-1 B-infected H9 cells, indicating the preservation of epitope structure in the ISCOMs matrix.
authors.affiliation: Program Resources, Inc., National Cancer Institute-Frederick Cancer Research Facility, MD 21701.
authors.forename: S W
authors.initials: SW
authors.lastname: Pyle
authors.forename: B
authors.initials: B
authors.lastname: Morein
authors.forename: J W
authors.initials: JW
authors.lastname: Bess
authors.forename: L
authors.initials: L
authors.lastname: Akerblom
authors.forename: P L
authors.initials: PL
authors.lastname: Nara
authors.forename: S M
authors.initials: SM
authors.lastname: Nigida
authors.forename: N W
authors.initials: NW
authors.lastname: Lerche
authors.forename: W G
authors.initials: WG
authors.lastname: Robey
authors.forename: P J
authors.initials: PJ
authors.lastname: Fischinger
authors.forename: L O
authors.initials: LO
authors.lastname: Arthur
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D015153:Blotting, Western; D002499:Centrifugation, Density Gradient; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D015483:HIV Antibodies / Q000096:biosynthesis*; D015699:HIV Envelope Protein gp120 / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D008253:Macaca mulatta; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D009500:Neutralization Tests; D011817:Rabbits; D011863:Radioimmunoassay
chemical_list: D000276:Adjuvants, Immunologic; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120