Detection and survival of group A rotavirus in a piggery.
Samples of dust, faeces and effluent were collected from a piggery and examined for group A rotavirus, using a commercial ELISA test, electron microscopy and inoculation of MA-104 cells. Rotavirus antigen was demonstrated in samples collected from farrowing and weaner rooms but not from fattener and sow houses. Rotavirus antigen was also detected in samples collected from a weaner room which had been free of piglets for three months. A cytopathic porcine rotavirus (British isolate SW20/21) was kept at room temperature for four months; it survived with titres reduced by 2 log10. These observations suggest that the environment of commercial piggeries is an important source of rotaviral infection for young piglets.
Fu
Z F
ZF
Department of Veterinary Pathology and Public Health, Massey University, Palmerston North, New Zealand.
Hampson
D J
DJ
Blackmore
D K
DK
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000382:microbiology; D006799:Housing, Animal; D008854:Microscopy, Electron; D012401:Rotavirus / Q000276:immunology / Q000302:isolation & purification* / Q000502:physiology / Q000648:ultrastructure; D012400:Rotavirus Infections / Q000382:microbiology / Q000662:veterinary*; D012722:Sewage; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D014779:Virus Replication
D000956:Antigens, Viral; D012722:Sewage