Examination of enterotoxigenic Escherichia coli H10407 (colonization factor antigen I+) by scanning electron microscopy with conductive staining.
We have used the scanning electron microscope to examine enterotoxigenic Escherichia coli H10407, which expresses colonization factor antigen I pili. The use of low accelerating voltages and conductive staining procedures allowed us to obtain images of colonization factor antigen I pili and other structural details which were obscured by conventional gold-coating techniques.
Sherburne
R
R
Department of Medical Microbiology and Infectious Diseases, University of Alberta, Edmonton, Canada.
Armstrong
G D
GD
D000371:Agglutination; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000032:analysis*; D001426:Bacterial Proteins / Q000032:analysis*; D004926:Escherichia coli / Q000648:ultrastructure*; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning / Q000379:methods
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C022924:colonization factor antigens; D036781:Fimbriae Proteins