Inhibition by islet-activating protein, pertussis toxin, of retinoic acid-induced differentiation of human leukemic (HL-60) cells.
Human promyelocytic leukemic (HL-60) cells were induced to differentiate into neutrophil- or macrophage-like cells by incubation of the cells with retinoic acid, dibutyryl cyclic AMP (Bt2cAMP) or phorbol 12-myristate 13-acetate (PMA). Differentiation was determined by an increase in the percentage of morphologically mature cells. The retinoic acid-induced differentiation of HL-60 cells was, but the Bt2cAMP- or PMA-induced one was not, inhibited by prior exposure of the cells to islet-activating protein (IAP), pertussis toxin. The IAP-induced inhibition was correlated with the toxin-catalyzed ADP-ribosylation of a membrane GTP-binding protein with a molecular mass of 40 kDa. Thus, the IAP-substrate GTP-binding protein appears to be involved in the retinoic acid-induced differentiation of HL-60 cells.
Tohkin
M
M
Department of Life Science, Faculty of Science, Tokyo Institute of Technology, Yokohama, Japan.
Iiri
T
T
Ui
M
M
Katada
T
T
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D002384:Catalysis; D002454:Cell Differentiation / Q000187:drug effects; D000242:Cyclic AMP / Q000494:pharmacology; D004347:Drug Interactions; D019204:GTP-Binding Proteins / Q000378:metabolism / Q000502:physiology; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000378:metabolism / Q000473:pathology*; D037342:Pertussis Toxin; D010703:Phorbol Esters / Q000494:pharmacology; D014212:Tretinoin / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
D010703:Phorbol Esters; D010566:Virulence Factors, Bordetella; D000246:Adenosine Diphosphate Ribose; D014212:Tretinoin; D000242:Cyclic AMP; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins