Nucleotide sequence of bovine herpesvirus type 1 glycoprotein gIII, a structural model for gIII as a new member of the immunoglobulin superfamily, and implications for the homologous glycoproteins of other herpesviruses.
The gene encoding bovine herpesvirus type 1 (BHV-1) glycoprotein gIII was mapped, cloned, and sequenced. The gene is situated between map units 0.122 and 0.135 and encodes a predicted protein of 521 amino acids. The identity of the sequenced gene has been verified previously by expression of immunologically authentic recombinant BHV-1 gIII (Fitzpatrick et al. (1988) J. Virol. 62, 4239-4248). Comparison of the BHV-1 gIII amino acid sequence with the homologous glycoproteins of other alphaherpesviruses revealed significant homology in the carboxy-terminal half of the molecules, including six invariant cysteine residues. A 96 amino acid domain with significant homology to class II major histocompatibility complex (MHC) antigen constant domains was identified in the conserved carboxy-terminal half of BHV-1 gIII. This domain is flanked by two other similarly sized domains which may be related to other immunoglobulin (Ig) superfamily domains. These homologies support a model for the structure of BHV-1 gIII as a new member of the Ig superfamily. Elements of the model may be applicable to the homologous glycoproteins of other herpesviruses and relevant to the immunobiology of herpesvirus infections.
Fitzpatrick
D R
DR
Department of Veterinary Microbiology, University of Saskatchewan, Saskatoon, Canada.
Babiuk
L A
LA
Zamb
T J
TJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D015342:DNA Probes; D004279:DNA, Viral / Q000235:genetics; D007242:Herpesvirus 1, Bovine / Q000235:genetics* / Q000276:immunology; D007136:Immunoglobulins / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011487:Protein Conformation; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000235:genetics* / Q000276:immunology
D015342:DNA Probes; D004279:DNA, Viral; D007136:Immunoglobulins; D014764:Viral Proteins; C046998:bovine herpesvirus type-1 glycoproteins