Density of beta adrenoceptors in rat heart and lymphocytes 48 hours and 7 days after acute myocardial infarction.
Down regulation of the beta adrenoceptor is thought to play an important role in the diminished response to catecholamines in heart failure. beta Adrenoceptor densities were measured on membrane homogenates of rat right ventricle and lymphocytes 48 h or 7 d after experimental myocardial infarction, and in rats exposed to a continuous infusion of isoprenaline (400 micrograms.kg-1.h1). The performance of the rat hearts was also evaluated 48 h post infarction in an isolated retrograde perfused heart preparation. In contrast to a 60% down regulation in right ventricle and a 20% down regulation in lymphocyte membranes after isoprenaline infusion, there was no change in right ventricle and lymphocyte beta adrenoceptor densities after myocardial infarction. Left ventricular contractile performance was significantly depressed 48 h after myocardial infarction. Mean basal left ventricular pressure decreased from 108(SEM 3) to 63(4) mm Hg while the maximal response to dobutamine was decreased from 204(4) to 105(12) mm Hg (n = 8). No correlation was found between the receptor densities of right ventricular and lymphocyte membranes. We conclude that diminished response to beta sympathomimetics after myocardial infarction cannot be attributed to a loss of surface beta adrenoceptors, and that the lymphocyte beta adrenoceptor does not provide an adequate system to monitor small receptor changes on the myocardium.
Vleeming
W
W
Department of Pharmacotherapy, Faculty of Pharmacy, University of Utrecht, The Netherlands.
van der Wouw
P A
PA
te Biesebeek
J D
JD
van Rooij
H H
HH
Wemer
J
J
Porsius
A J
AJ
D000818:Animals; D002462:Cell Membrane / Q000032:analysis; D004280:Dobutamine / Q000494:pharmacology; D006321:Heart / Q000187:drug effects / Q000503:physiopathology; D006352:Heart Ventricles / Q000032:analysis / Q000648:ultrastructure; D007545:Isoproterenol / Q000494:pharmacology; D008214:Lymphocytes / Q000032:analysis* / Q000648:ultrastructure; D008297:Male; D009203:Myocardial Infarction / Q000378:metabolism* / Q000503:physiopathology; D009206:Myocardium / Q000032:analysis* / Q000648:ultrastructure; D010869:Pindolol / Q000031:analogs & derivatives / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011943:Receptors, Adrenergic, beta / Q000032:analysis* / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors
D011943:Receptors, Adrenergic, beta; D004280:Dobutamine; C029247:3-iodopindolol; D010869:Pindolol; D007545:Isoproterenol