[Use of an immunoblotting method for identifying the individual proteins in the antigenic complexes of Bordetella pertussis].
The composition of antigenic complexes isolated from the supernatant fluid of B. pertussis culture has been studied by means of immunoblotting techniques. In preparations obtained from B. pertussis strains 305 and 475 fragments of the molecule of fimbrial hemagglutinin, three subunits of B. pertussis toxin and agglutinogens 2 and 3 have been detected with the use of antisera to B. pertussis protective substances.
Semina
I E
IE
Iankina
Z K
ZK
D000373:Agglutinins / Q000032:analysis; D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis* / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000032:analysis* / Q000302:isolation & purification; D001886:Bordetella pertussis / Q000032:analysis / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D010861:Fimbriae, Bacterial / Q000276:immunology; D006388:Hemagglutinins / Q000032:analysis; D015151:Immunoblotting / Q000379:methods; D008970:Molecular Weight; D037342:Pertussis Toxin; D011817:Rabbits; D010566:Virulence Factors, Bordetella / Q000032:analysis
D000373:Agglutinins; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D006388:Hemagglutinins; D010566:Virulence Factors, Bordetella; D037342:Pertussis Toxin
Ispol'zovanie metoda immunoblottinga dlia identifikatsii individual'nykh belkov v antigennykh kompleksakh kokliushnogo mikroba.