New B-lymphocyte-specific enhancer-binding protein.
We report the discovery of a new B-lymphocyte-specific enhancer-binding protein. A series of gel retardation assays using fragments that scan the -2172 to -1180 region of the major histocompatibility complex class II gene E alpha reveal a site (W) that serves as the recognition sequence for two nuclear proteins, one B-cell restricted and the other ubiquitously occurring. Certain characteristics of the NF-W1 and NF-W2 pair recall the OTF-2/NF-A2 and OTF-1/NF-A1 pair that binds to the immunoglobulin octamer, but we demonstrate that the two protein pairs are distinguishable by several criteria. NF-W1 and NF-W2 interact differentially with their common GTTGCATC binding site, display a different affinity for it, and have molecular weights that differ by about 20,000. Yet, proteolysis experiments and cross-linking analyses indicate that the two W complexes show structural relatedness.
Dorn
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A
Laboratoire de Génétique Moléculaire des Eucaryotes, Centre National de La Recherche Scientifique, Strasbourg, France.
Benoist
C
C
Mathis
D
D
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D001483:Base Sequence; D002478:Cells, Cultured; D003432:Cross-Linking Reagents; D004268:DNA-Binding Proteins / Q000235:genetics*; D004742:Enhancer Elements, Genetic; D005801:Genes, Homeobox; D005802:Genes, MHC Class II; D007700:Kinetics; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic
D003432:Cross-Linking Reagents; D004268:DNA-Binding Proteins