Transformation and expression of a cloned delta-endotoxin gene in Bacillus thuringiensis.
A shuttle vector containing the replication region of a resident plasmid of B. thuringiensis, was used to determine the conditions allowing efficient transformation of B. thuringiensis by electroporation. Using this plasmid a delta-endotoxin gene was cloned and expressed both in Escherichia coli and B. thuringiensis. It was shown that this gene was poorly expressed in the wild type situation whereas after cloning in acrystalliferous strains of B. thuringiensis large amounts of crystal protein were obtained.
Lereclus
D
D
Unité de Biochimie Microbienne, Institut Pasteur, Paris, France.
Arantès
O
O
Chaufaux
J
J
Lecadet
M
M
D001413:Bacillus thuringiensis / Q000235:genetics*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D003001:Cloning, Molecular; D003460:Crystallization; D004560:Electricity; D004731:Endotoxins / Q000096:biosynthesis / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D005822:Genetic Vectors; D006460:Hemolysin Proteins; D007306:Insecticides / Q000096:biosynthesis*; D010957:Plasmids; D015183:Restriction Mapping; D014169:Transformation, Bacterial
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; C030956:insecticidal crystal protein, Bacillus Thuringiensis