Ultrastructural localization of viral DNA in thin sections of herpes simplex virus type 1 infected cells by in situ hybridization.
We have used in situ hybridization at the ultrastructural level to localize non-encapsidated and encapsidated herpes simplex virus type 1 (HSV-1) genomes in nuclei of infected rabbit fibroblasts. A biotinylated cloned subgenomic HSV DNA fragment was used as hybridization probe. The probe hybridized to the viral DNA accessible at the surface of Lowicryl sections was revealed by immunogold labeling. Non-encapsidated viral DNA was detected exclusively within the virus-induced central region of 4 h to 17 h infected nuclei. Localization of the probe either near the nuclear envelope or within marginated host chromatin was found only on HSV DNA which was packaged into viral nucleoids. The use in parallel of in situ hybridization with specific staining for DNA and autoradiography after tritiated thymidine incorporation, followed by either conventional fixation of the cells or the nucleoprotein loosening procedure, indicated that non-encapsidated viral DNA and marginated host chromatin formed two juxtaposed compartments without interpenetration even after experimentally produced mild dispersion of the nuclear components.
Puvion-Dutilleul
F
F
Groupe de Laboratoires de l'Institut de Recherches Scientifiques sur le Cancer, CNRS (ER272), Villejuif/France.
Puvion
E
E
D000818:Animals; D002478:Cells, Cultured; D004279:DNA, Viral / Q000378:metabolism / Q000648:ultrastructure*; D005347:Fibroblasts / Q000648:ultrastructure*; D006566:Herpesviridae Infections / Q000235:genetics* / Q000473:pathology; D008854:Microscopy, Electron; D009693:Nucleic Acid Hybridization; D011817:Rabbits; D018139:Simplexvirus; D013997:Time Factors
D004279:DNA, Viral