title: Association of DNA aneuploidy with human papillomavirus-induced malignant transformation of sinonasal transitional papillomas.
abstract: The nuclear DNA content of 19 transitional papillomas of the sinonasal region and 9 maxillary squamous cell carcinomas was studied by flow cytometry; the presence of human papillomavirus (HPV) DNA types 11 and 16 was determined by the in situ hybridization technique from paraffin-embedded tissue. Thirteen (68%) of the papillomas and none of the carcinomas contained HPV genome. Six (32%) of the papillomas and 4 (44%) of the carcinomas had an aneuploid DNA content. The relative DNA content (DNA index) of the aneuploid maxillary carcinomas was larger than that of aneuploid papillomas (p = 0.004). Three of the papillomas underwent malignant transformation, all three of which contained HPV type 16 DNA; two were also aneuploid. Data indicate that papillomas containing HPV type 16 DNA have a tendency (p = 0.06) to undergo malignant transformation, and that this tendency is greater if DNA aneuploidy or HPV type 11 DNA is also present (p = 0.02).
authors.affiliation: Department of Pathology, University Central Hospital, Turku, Finland.
authors.forename: P J
authors.initials: PJ
authors.lastname: Klemi
authors.forename: H
authors.initials: H
authors.lastname: Joensuu
authors.forename: L
authors.initials: L
authors.lastname: Siivonen
authors.forename: E
authors.initials: E
authors.lastname: Virolainen
authors.forename: S
authors.initials: S
authors.lastname: Syrjänen
authors.forename: K
authors.initials: K
authors.lastname: Syrjänen
mesh_terms: D000782:Aneuploidy; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D004273:DNA, Neoplasm / Q000648:ultrastructure; D005434:Flow Cytometry; D006801:Humans; D010212:Papilloma / Q000235:genetics*; D027383:Papillomaviridae
chemical_list: D004273:DNA, Neoplasm