The effect of ferricyanide with iodoacetate in calcium-free solution on passive cation permeability in human red blood cells: comparison with the Gardos-effect and with the influence of PCMBS on passive cation permeability.
Freshly prepared human red blood cells incubated with 5 mM ferricyanide, 0.2 mM iodoacetate and 2 mM adenosine in the presence of 5 mM EGTA demonstrate comparable increases in Na+ and K+ permeability (ferricyanide effect). This effect is unrelated to the Ca2+-activated K+ channel (Gardos effect) since influx of Ca2+ from outside the cell is excluded. Also this effect is different from the non-specific Na+ and K+ permeability change elicited by PCMBS. These differences become obvious by using various reagents. For example, A23187 and quinidine exert opposite effects in Gardos and ferricyanide experiments, where A23187 and atebrin react oppositely in the latter and in PCMBS experiments. The ferricyanide effect described here does not involve formation of nonspecific channels. The change in Na+ permeability separately from K+ permeability under certain circumstances suggests a more specific effect.
Fuhrmann
G F
GF
Institut für Pharmakologie und Toxikologie, Philipps Universität Marburg, F.R.G.
Fehlau
R
R
Schneider
H
H
Knauf
P A
PA
D002731:4-Chloromercuribenzenesulfonate / Q000494:pharmacology*; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium; D002463:Cell Membrane Permeability / Q000187:drug effects*; D004910:Erythrocyte Membrane / Q000187:drug effects*; D005292:Ferricyanides / Q000494:pharmacology*; D006801:Humans; D007461:Iodoacetates / Q000494:pharmacology*; D019807:Iodoacetic Acid; D010663:Phenylmercury Compounds / Q000494:pharmacology*; D015221:Potassium Channels / Q000187:drug effects; D011796:Quinacrine / Q000494:pharmacology; D011802:Quinidine / Q000494:pharmacology; D015222:Sodium Channels / Q000187:drug effects; D012996:Solutions; D014638:Vanadates / Q000494:pharmacology
D005292:Ferricyanides; D007461:Iodoacetates; D010663:Phenylmercury Compounds; D015221:Potassium Channels; D015222:Sodium Channels; D012996:Solutions; C007931:hexacyanoferrate III; D000001:Calcimycin; D014638:Vanadates; D002731:4-Chloromercuribenzenesulfonate; D011796:Quinacrine; D011802:Quinidine; D002118:Calcium; D019807:Iodoacetic Acid