title: Further characterization of four lipocortins from human peripheral blood mononuclear cells.
abstract: Four calcium and phospholipid binding proteins purified from mononuclear cells were characterized for PKC and EGF phosphorylation, actin binding capacity, and partial tissue distribution. Those named 35K, 32K, and 73K are equivalent, respectively, to lipocortin III, endonexin II and the 67 kDa calelectrin; 36K is a fragment of 73K. After purification, 35K and 73K were phosphorylated by protein kinase C in vitro but 36K nor 32K were not. None were phosphorylated by the epidermal growth factor receptor kinase in vitro; 73K bound F-actin in a calcium-dependent manner, whereas 35K, 36K, and 32K did not. Using Western blotting analysis, 32K and 73K were detected in high amounts in human lymphocytes, monocytes, liver, and placenta and in rat adrenal medulla; but 32K was not detected in polymorphonuclear cells, and 36K and 35K were detected in high amounts only, respectively, in human blood lymphocytes and polymorphonuclear cells. Thus, 32K and 73K appear to have a wide tissue distribution, whereas 35K has a much more restricted distribution.
authors.affiliation: INSERM UA 285, Institut Pasteur, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Coméra
authors.forename: B
authors.initials: B
authors.lastname: Rothhut
authors.forename: J C
authors.initials: JC
authors.lastname: Cavadore
authors.forename: I
authors.initials: I
authors.lastname: Vilgrain
authors.forename: C
authors.initials: C
authors.lastname: Cochet
authors.forename: E
authors.initials: E
authors.lastname: Chambaz
authors.forename: F
authors.initials: F
authors.lastname: Russo-Marie
mesh_terms: D000199:Actins / Q000378:metabolism; D017302:Annexins; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002846:Chromatography, Affinity; D066246:ErbB Receptors; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008970:Molecular Weight; D010740:Phospholipases / Q000037:antagonists & inhibitors*; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D000199:Actins; D017302:Annexins; D002135:Calcium-Binding Proteins; D066246:ErbB Receptors; D011505:Protein-Tyrosine Kinases; D011493:Protein Kinase C; D010740:Phospholipases; D002118:Calcium