Malignant transformation of human bladder epithelial cells by DNA transfection with the v-raf oncogene.
Transfection of the v-raf oncogene into immortalized, nontumorigenic human bladder epithelial cells resulted in the isolation of two tumorigenic transformants. Both were identified as human and of the same origin as the parent cell line by human leukocyte antigen typing and Southern blot analysis. Both the primary tumorigenic transfectants and the cell lines established from the induced tumors expressed v-raf mRNA and v-raf protein. In both tumorigenic transformants the level of c-myc mRNA was enhanced compared with that of the parent cell line.
Skouv
J
J
Department of Environmental Carcinogenesis, Fibiger Institute, Copenhagen, Denmark.
Ottesen
S
S
Mark
G
G
Autrup
H
H
D000818:Animals; D015197:Carcinogenicity Tests; D002460:Cell Line; D002472:Cell Transformation, Viral; D015342:DNA Probes; D004848:Epithelium; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D027384:Oncogene Proteins v-raf; D009857:Oncogenes; D010957:Plasmids; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger / Q000096:biosynthesis; D012191:Retroviridae Proteins / Q000235:genetics; D009053:Sarcoma Viruses, Murine / Q000235:genetics; D014162:Transfection; D001743:Urinary Bladder / Q000166:cytology
D015342:DNA Probes; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D012191:Retroviridae Proteins; D027384:Oncogene Proteins v-raf