title: Comparison of western blot analysis and immunocytochemical detection of P-glycoprotein in multidrug resistant cells.
abstract: A sensitive immunocytochemical technique was developed to detect a 170,000 dalton cell membrane glycoprotein (P-gp) in cell lines resistant to vincristine and vinblastine with varying degrees of resistance. P-gp was shown very clearly using the C219 monoclonal antibody and immunocytochemical detection with either antialkaline phosphate or peroxidase-antiperoxidase with silver gold intensification. There was good correlation between the results obtained with immunocytochemical detection of P-gp in single cells and Western blot analysis. The technique is easily performed and can detect P-gp in relatively small numbers of cells that Western blot analysis could miss and is suitable for clinical application.
authors.affiliation: Department of Clinical Oncology, Royal North Shore Hospital, St Leonards, New South Wales, Australia.
authors.forename: M L
authors.initials: ML
authors.lastname: Friedlander
authors.forename: D R
authors.initials: DR
authors.lastname: Bell
authors.forename: J
authors.initials: J
authors.lastname: Leary
authors.forename: R A
authors.initials: RA
authors.lastname: Davey
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D015153:Blotting, Western; D002460:Cell Line; D004351:Drug Resistance; D006801:Humans; D007124:Immunoenzyme Techniques; D008562:Membrane Glycoproteins / Q000032:analysis*; D014407:Tumor Cells, Cultured / Q000032:analysis* / Q000187:drug effects; D014747:Vinblastine / Q000494:pharmacology; D014750:Vincristine / Q000494:pharmacology
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D008562:Membrane Glycoproteins; D014750:Vincristine; D014747:Vinblastine