title: Evidence for a role of virulent human immunodeficiency virus (HIV) variants in the pathogenesis of acquired immunodeficiency syndrome: studies on sequential HIV isolates.
abstract: Sequential human immunodeficiency virus (HIV) isolates, recovered from a panel of longitudinally collected peripheral blood mononuclear cells obtained from 20 initially asymptomatic HIV-seropositive homosexual men, were studied for differences in replication rate, syncytium-inducing capacity, and host range. Eleven individuals remained asymptomatic; nine progressed to acquired immunodeficiency syndrome (AIDS) or AIDS-related complex (ARC) at the time point at which the last HIV isolate was obtained. In 16 individuals, only non-syncytium-inducing (NSI) isolates, with a host range restricted to mononuclear cells, were observed. From four individuals, high-replicating, syncytium-inducing (SI) isolates that could be transmitted to the H9, RC2A, and U937 cell lines were recovered. From two of these four individuals, SI isolates were obtained throughout the observation period. In the two others, a transition from NSI to SI HIV isolates was observed during the period of study. Three of these four individuals developed ARC or AIDS 9 to 15 months after the first isolation of an SI isolate. With the exception of the two individuals in whom a transition from NSI to SI isolates was observed, within a given individual the replication rate of sequential HIV isolates was constant. A significant correlation was found between the mean replication rate of isolates obtained from an individual and the rate of CD4+ cell decrease observed in this individual. In individuals with low-replicating HIV isolates, no significant CD4+ cell loss was observed. In contrast, recovery of high-replicating isolates, in particular when these were SI isolates, was associated with rapid decline of CD4+ cell numbers and development of ARC or AIDS. These findings indicate that variability in the biological properties of HIV isolates is one of the factors influencing the course of HIV infection.
authors.affiliation: Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam.
authors.forename: M
authors.initials: M
authors.lastname: Tersmette
authors.forename: R A
authors.initials: RA
authors.lastname: Gruters
authors.forename: F
authors.initials: F
authors.lastname: de Wolf
authors.forename: R E
authors.initials: RE
authors.lastname: de Goede
authors.forename: J M
authors.initials: JM
authors.lastname: Lange
authors.forename: P T
authors.initials: PT
authors.lastname: Schellekens
authors.forename: J
authors.initials: J
authors.lastname: Goudsmit
authors.forename: H G
authors.initials: HG
authors.lastname: Huisman
authors.forename: F
authors.initials: F
authors.lastname: Miedema
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology*; D015496:CD4-Positive T-Lymphocytes / Q000032:analysis; D002459:Cell Fusion; D014644:Genetic Variation; D006678:HIV / Q000302:isolation & purification / Q000472:pathogenicity*; D006679:HIV Seropositivity / Q000382:microbiology*; D006801:Humans; D008297:Male; D011379:Prognosis; D015183:Restriction Mapping; D014779:Virus Replication