Mapping of the human complement factor I gene to 4q25.
A detailed genetic and physical map of human complement factor I (IF) using somatic cell hybrids, in situ hybridization, and genetic linkage is reported. The gene has been localized to band 4q25. The order GC-INP10-ADH3-EGF-IF-IL2-MNS is proposed for genes on 4q on the basis of genetic and physical mapping techniques. A BclI polymorphism found with the IF probe demonstrated a maternal origin for a de novo deletion of chromosome 4 that was used in physically mapping the gene. The genetic and physical distances around band 4q24 suggest that 1 cM is approximately 1.2 million bp of DNA. This work provides a useful addition to the map of 4q.
Shiang
R
R
Department of Pediatrics, University of Iowa, Iowa City 52242.
Murray
J C
JC
Morton
C C
CC
Buetow
K H
KH
Wasmuth
J J
JJ
Olney
A H
AH
Sanger
W G
WG
Goldberger
G
G
D002874:Chromosome Mapping; D002894:Chromosomes, Human, Pair 4; D017244:Complement Factor I; D008040:Genetic Linkage; D006801:Humans; D006822:Hybrid Cells; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length; D012697:Serine Endopeptidases / Q000235:genetics*
D012697:Serine Endopeptidases; D017244:Complement Factor I