title: Release of taurine from cultured cerebellar granule cells and astrocytes: co-release with glutamate.
abstract: The properties of the release of preloaded [3H]taurine and endogenous taurine were studied with cultured cerebellar granule cells (7-8 days in vitro) and astrocytes (14-15 days in vitro) from the rat. The spontaneous release of taurine from both cell types was slow. The release from both neurons and astrocytes was significantly enhanced by 0.1 mM veratridine, the stimulatory effect being more pronounced in granule cells than in astrocytes. No homo or heteroexchange with extracellularly added taurine or its structural analogues could be detected, suggesting that the efflux is probably not mediated via the membrane transport sites. Kainate stimulated the release more from granule cells than from astrocytes, the effect apparently being mediated by kainate-sensitive receptors. Depolarization of cell membranes by 50 mM K+ induced co-release of endogenous taurine and glutamate from both cell types. Preloaded [3H]taurine was readily released from astrocytes by potassium stimulation. Stimulated release occurred from granule cells if they had been cultured for 4 days with the label but not from the cells preloaded for only 15 min.
authors.affiliation: Tampere Brain Research Center, Department of Biomedical Sciences, University of Tampere, Finland.
authors.forename: I
authors.initials: I
authors.lastname: Holopainen
authors.forename: P
authors.initials: P
authors.lastname: Kontro
authors.forename: S S
authors.initials: SS
authors.lastname: Oja
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D007608:Kainic Acid / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013654:Taurine / Q000378:metabolism* / Q000493:pharmacokinetics; D014701:Veratridine / Q000494:pharmacology
chemical_list: D005971:Glutamates; D013654:Taurine; D018698:Glutamic Acid; D014701:Veratridine; D011188:Potassium; D007608:Kainic Acid