In vitro scavenger effect of dihydroquinoline type derivatives in different free radical generating systems.
The non-toxic and water soluble dihydroquinoline type antioxidants: CH 402 (Na-2,2-dimethyl-1,2-dihydroquinoline-4-yl methane sulphonate) and MTDQ-DA (6,6-methylene bis 2,2-dimethyl-4-methane sulphonic acid: Na-1,2-dihydroquinoline) were studied in various in vitro tests in which oxygen free radicals were generated. Both compounds were shown to scavenge superoxide radical anions O2- produced in aqueous solution by pulse radiolysis with rate constants k (O2- + MTDQ-DA) = 4.10(8) dm3 mol-1 s-1 and k (O2- + CH 402) = 1.5.10(7) dm3 mol-1 s-1. CH 402 and MTDQ-DA reduced the H2O2 produced in the glucose-glucose oxidase reaction, which was detected by the luminol + hemin reaction with a chemiluminometric method. The dihydroquinoline type substrates inhibited the NADPH-induced and Fe3+ - stimulated lipid peroxidation and the ascorbic acid-induced non-enzymatic peroxidation pathways in microsomal fractions of rat and mouse liver.
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Second Department of Medicine, Semmelweis Medical University, Budapest, Hungary.
György
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Zsinka
A J
AJ
Biacs
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P
Földiák
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Feher
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J
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D005609:Free Radicals; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects; D008163:Luminescent Measurements; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D011804:Quinolines / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013481:Superoxides / Q000378:metabolism
D000975:Antioxidants; D005609:Free Radicals; D011804:Quinolines; D013481:Superoxides; C059107:CH 402; C044964:6,6'-methylenebis(2,2-dimethyl-4-methanesulfonic acid-1,2-dihydroquinoline)