title: Selection of human chromosome 21-specific DNA probes for genetic analysis in Alzheimer's dementia and Down syndrome.
abstract: We used a mouse-human somatic cell hybrid to construct a chromosome 21-enriched library in phage vector EMBL4. In all, 35 phage clones containing human inserts were identified by differential screening with total human and mouse DNA. Whole recombinant phages were regionally mapped on chromosome 21 by Southern blot analysis using competitive hybridisation conditions to block repetitive sequences. Ten phage clones mapped proximal to a translocation breakpoint in band 21q21.2, while 25 mapped distal to this point. Three of the phage clones identify restriction fragment length polymorphisms. Polymorphic chromosome 21 markers may be useful in the genetic analysis of Alzheimer's dementia and Down syndrome.
authors.affiliation: Department of Biochemistry, University of Antwerp, Belgium.
authors.forename: G
authors.initials: G
authors.lastname: Van Camp
authors.forename: P
authors.initials: P
authors.lastname: Stinissen
authors.forename: W
authors.initials: W
authors.lastname: Van Hul
authors.forename: H
authors.initials: H
authors.lastname: Backhovens
authors.forename: A
authors.initials: A
authors.lastname: Wehnert
authors.forename: A
authors.initials: A
authors.lastname: Vandenberge
authors.forename: C
authors.initials: C
authors.lastname: Van Broeckhoven
mesh_terms: D000544:Alzheimer Disease / Q000235:genetics*; D000818:Animals; D002891:Chromosomes, Human, Pair 21 / Q000648:ultrastructure*; D003001:Cloning, Molecular; D015342:DNA Probes; D004274:DNA, Recombinant; D004314:Down Syndrome / Q000235:genetics*; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D012150:Polymorphism, Restriction Fragment Length; D014178:Translocation, Genetic
chemical_list: D015342:DNA Probes; D004274:DNA, Recombinant; D005819:Genetic Markers