Cholesterol and bile acid synthesis in Hep G2 cells. Metabolic effects of 26- and 7 alpha-hydroxycholesterol.
1. Using a human hepatoma (Hep G2) cell line that continually synthesizes 3 beta-hydroxy-5-cholenoic acid, lithocholic acid, chenodeoxycholic acid and cholic acid we have determined the metabolism and biological effects of 26-hydroxycholesterol and 7 alpha-hydroxycholesterol. 2. Addition of 26-hydroxycholesterol to the medium (6 microM) downregulated cholesterol and chenodeoxycholic acid synthesis. 3. The predominant metabolite of 26-hydroxycholesterol was 3 beta-hydroxy-5-cholenoic acid. 4. Cholesterol synthesis was not affected by the addition of 7 alpha-hydroxycholesterol (6 and 12 microM). The predominant metabolite of 7 alpha-hydroxycholesterol was chenodeoxycholic acid. 5. In Hep G2 cells 7 alpha-hydroxylation of 26-hydroxycholesterol is not well expressed.
Javitt
N B
NB
Division of Hepatic Diseases, New York University Medical Center, NY 10016.
Budai
K
K
D001647:Bile Acids and Salts / Q000096:biosynthesis*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism; D002460:Cell Line; D002635:Chenodeoxycholic Acid / Q000096:biosynthesis; D002784:Cholesterol / Q000096:biosynthesis*; D002793:Cholic Acids / Q000378:metabolism; D006801:Humans; D006888:Hydroxycholesterols / Q000494:pharmacology*; D008113:Liver Neoplasms / Q000378:metabolism*
D001647:Bile Acids and Salts; D002793:Cholic Acids; D006888:Hydroxycholesterols; D002635:Chenodeoxycholic Acid; C007042:cholest-5-ene-3 beta,26-diol; C014596:3 beta-hydroxy-delta 5-cholenic acid; C011724:cholest-5-en-3 beta,7 alpha-diol; D002784:Cholesterol