Restriction endonucleases do not induce sister-chromatid exchanges in Chinese hamster ovary cells.
Bacterial restriction enzymes offer the unique opportunity to determine the biological and cytogenetic consequences of DNA double-strand breakage. To examine the role of various types of breaks in sister-chromatid exchange (SCE) formation, we used restriction enzymes with different recognition sequences and different cutting frequencies to generate DNA double-strand breaks in Chinese hamster ovary (CHO) cells. The restriction enzymes were introduced by electroporation into exponentially growing cells during the second replication cycle in bromodeoxyuridine, and SCEs were analyzed at mitosis. Contrary to results reported by others, we found no increase in SCE frequency in cells exposed to restriction enzymes despite the presence of numerous cells with chromatid aberrations. These data suggest that DNA double-strand breaks do not lead to SCE formation.
Morgan
W F
WF
Laboratory of Radiobiology and Environmental Health, University of California, San Francisco 94143-0750.
Chung
H W
HW
Phillips
J W
JW
Winegar
R A
RA
D000818:Animals; D002460:Cell Line; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000032:analysis; D004249:DNA Damage; D004262:DNA Restriction Enzymes / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D005260:Female; D010053:Ovary; D012854:Sister Chromatid Exchange
D004247:DNA; D004262:DNA Restriction Enzymes