Use of synthetic oligodeoxyribonucleotides for type-specific identification of coxsackie B viruses.
Synthetic oligodeoxyribonucleotides were used for type-specific identification of members of the coxsackie B virus group by nucleic acid hybridization. Two pairs of oligonucleotide chains were constructed based on nucleotide sequences in the VP1 regions of coxsackieviruses B3 and B4. Each labelled probe had a length of 24 nucleotides. The results showed that the oligonucleotide hybridized in a type-specific manner when assayed with extracts from cells infected with all different coxsackie B viruses. A method based on similar principles may thus be used for enterovirus typing.
Alksnis
M
M
Department of Medical Genetics, Uppsala University, Sweden.
Lindberg
M
M
Stålhandske
P
P
Hultberg
H
H
Pettersson
U
U
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D029822:Enterovirus B, Human / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides
D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides