The occurrence of long ribosomal transcripts homologous to type I insertions in bobbed mutants of Drosophila melanogaster.
In Drosophila melanogaster up to two thirds of the rDNA genes contain insertion sequences of two types in the 28S coding region. Comparison of the ribosomal insertion transcripts in the wild type and in two bobbed mutants reared at two temperatures showed that the level of type I transcripts is dependent on both the number of genes with type I insertions in the bobbed loci and the intensity of bobbed phenotype. Importantly, a long transcript of 8.7 kb hybridized to the ribosomal probe, the INS I probe and also to the restriction fragment of the rDNA downstream of the point of insertion was found in one bobbed mutant. This result and also those from sandwich hybridization indicate that some interrupted ribosomal genes are functional.
Makni
M
M
Marrakchi
M
M
Prud'homme
N
N
D000818:Animals; D004251:DNA Transposable Elements; D004275:DNA, Ribosomal / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D008297:Male; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D014158:Transcription, Genetic
D004251:DNA Transposable Elements; D004275:DNA, Ribosomal