Neutrophil superoxide production measurement by means of stable nitroxide radical accumulation detected by ESR.
A new assay for superoxide radicals is based on the interaction of hydroxylamine (1-oxy-2,2,6,6-tetramethyl-4-oxopiperidine) with superoxide, giving rise to a stable nitroxide radical. Working concentration ranges of hydroxylamine and cells are determined. It was shown that the amount of superoxide generated was proportional to the concentration of nitroxide radicals. The sensitivity and specificity of the proposed assay were compared to chemiluminescence and cytochrome-c reduction.
Osipov
A N
AN
Second N.I. Pirogov Moscow Medical Institute, Academy of Sciences, U.S.S.R.
Vartanyan
L S
LS
Azizova
O A
OA
Vladimirov
Y A
YA
D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D005609:Free Radicals; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D009589:Nitrogen Oxides / Q000097:blood*; D013482:Superoxide Dismutase / Q000097:blood; D013481:Superoxides / Q000097:blood*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D005609:Free Radicals; D009589:Nitrogen Oxides; D013481:Superoxides; D013482:Superoxide Dismutase; C039900:nitroxyl; D013755:Tetradecanoylphorbol Acetate