title: DNA polymorphism analysis of hereditary multiple exostoses in horses.
abstract: Genomic DNA polymorphisms obtained by restriction fragment-length polymorphism from healthy horses and horses with hereditary multiple exostoses were analyzed. These DNA were digested by 12 restriction enzymes and were hybridized against 6 isotopically labeled oncogene probes. Hybridization was not detected with the viral oncogene, v-ras, which indicated this oncogene was absent in the equine genome. Oncogenes (c-raf-1, c-fes, c-myb, c-myc, and c-sis) were present and had similar hybridization patterns and signal intensities in DNA from healthy horses and horses with hereditary multiple exostoses. Unique and distinct restriction fragment-length polymorphisms were detected with the c-raf-1 probe only in BamHI- and PstI-digested equine DNA.
authors.affiliation: Molecular Biology/Biochemistry Program, Utah State University, Logan 84322-5500.
authors.forename: J K
authors.initials: JK
authors.lastname: Li
authors.forename: B K
authors.initials: BK
authors.lastname: Moloney
authors.forename: J L
authors.initials: JL
authors.lastname: Shupe
authors.forename: E J
authors.initials: EJ
authors.lastname: Gardner
authors.forename: N C
authors.initials: NC
authors.lastname: Leone
authors.forename: Y
authors.initials: Y
authors.lastname: Elsner
mesh_terms: D000818:Animals; D004247:DNA / Q000235:genetics*; D005097:Exostoses, Multiple Hereditary / Q000235:genetics / Q000662:veterinary*; D005260:Female; D006734:Horse Diseases / Q000235:genetics*; D006736:Horses / Q000235:genetics; D008297:Male; D009693:Nucleic Acid Hybridization; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D004247:DNA