[Nuclease-aided simulation of the action of radiation on Chinese hamster fibroblasts. The role of double-stranded DNA breaks induced by restrictases in initiating chromosomal disorders].
Comparison was made between the effectiveness of restrictases inducing double-strand DNA breaks with blunt (Hae III and Eco RV) and cohesive (Hind III and Sal I) ends and that of gamma-radiation on the initiation of chromosome aberrations. The analysis of the spectrum of chromosome aberrations induced in the presence or absence of DNA repair inhibitors, as well as the study of the pattern of cell distribution by the number of DNA breaks per cell showed that the decisive role in the initiation of chromosome mutagenesis is played by the localization of the break in certain sequences of target DNA rather than the type of the break.
Zaichkina
S I
SI
Rozanova
O M
OM
Ganassi
E E
EE
D000818:Animals; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000187:drug effects / Q000528:radiation effects*; D004249:DNA Damage; D004262:DNA Restriction Enzymes / Q000494:pharmacology*; D004277:DNA, Single-Stranded / Q000187:drug effects / Q000528:radiation effects; D003851:Deoxyribonucleases / Q000528:radiation effects*; D005347:Fibroblasts / Q000187:drug effects / Q000528:radiation effects*; D005720:Gamma Rays; D008954:Models, Biological
D004277:DNA, Single-Stranded; D004247:DNA; D003851:Deoxyribonucleases; D004262:DNA Restriction Enzymes
Modelirovanie s pomoshch'iu nukleaz deĭstviia radiatsii na fibroblasty kitaĭskogo khomiachka. O roli dvunitevykh razryvov DNK, indutsirovannykh restriktazami, v initsiatsii khromosomnykh narusheniĭ.