Chemical integrity of [3H]GABA used in binding studies.
A method which is claimed to be able to determine the proportion of "true" GABA within radiolabeled GABA used in binding studies was tested using [3H]GABA. The method was found to be unsuitable for 3H-labeled GABA and, furthermore, both theoretical considerations and the present experimental data indicated that it could also produce misleading results with [14C]GABA.
Balcar
V J
VJ
Department of Anatomy, University of Sydney, N.S.W., Australia.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D011786:Quality Control; D051381:Rats; D011919:Rats, Inbred Strains; D011963:Receptors, GABA-A / Q000378:metabolism; D014316:Tritium; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D011963:Receptors, GABA-A; D014316:Tritium; D005680:gamma-Aminobutyric Acid