title: Use of cultured renal epithelial cells for the study of cisplatin toxicity.
abstract: The nephrotoxicity of cisplatin was evaluated in an in vitro system with an established cell line of normal rat kidney, NRK-52E. Leakage of enzymes from the cells into the culture medium increased when they were exposed to 1 microM of cisplatin for 72 hr. The level of lipid peroxides increased in the cells after 48 hr of exposure to cisplatin; the increase was more rapid than the enzyme leakage. This culture system can be used to evaluate drug-induced nephrotoxicity and its mechanism.
authors.affiliation: Division of Pharmacology, Osaka University of Pharmaceutical Sciences, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Fukuishi
authors.forename: M
authors.initials: M
authors.lastname: Gemba
mesh_terms: D000118:Acetylglucosaminidase / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D002945:Cisplatin / Q000633:toxicity*; D004847:Epithelial Cells; D007668:Kidney / Q000166:cytology* / Q000201:enzymology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D051381:Rats; D005723:gamma-Glutamyltransferase / Q000378:metabolism
chemical_list: D008315:Malondialdehyde; D007770:L-Lactate Dehydrogenase; D005723:gamma-Glutamyltransferase; D000118:Acetylglucosaminidase; D002945:Cisplatin