title: Induction by the calcium ionophore A23187 of a protective effect against cell injury in cultured gastric mucosal cells.
abstract: An intrinsic protective mechanism against cell injury seems to exist in cultured gastric mucosal cells. Cells, isolated from the stomachs of 10- to 12-day-old rats and subcultured, were examined for damage by the erythrosine B dye exclusion test. Pretreatment with 5 microM A23187 (a calcium ionophore) diminished the cell damage induced by acidified medium (pH 3.5) or 8 mM aspirin (pH 5.0). The effect of A23187 appeared 4 hr after its addition and was reversible. Protection by A23187 against cell injury diminished in the absence of extracellular Ca2+ and was dependent on Ca2+ concentration. An increase in intracellular Ca2+ may induce cell resistance against injury in cultured gastric mucosal cells.
authors.affiliation: Shionogi Research Laboratories, Shionogi & Co., Ltd., Osaka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Mizushima
mesh_terms: D000818:Animals; D000001:Calcimycin / Q000494:pharmacology*; D002478:Cells, Cultured; D004923:Erythrosine; D005744:Gastric Acid / Q000378:metabolism / Q000502:physiology; D005753:Gastric Mucosa / Q000187:drug effects*; D006863:Hydrogen-Ion Concentration; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000001:Calcimycin; D004923:Erythrosine