Transcriptional activation is not responsible for increased levels of autonomously expressed simian virus 40 T-antigen in herpes simplex virus-infected cells.
Herpes simplex virus type 1 (HSV-1) superinfection of CV-1 cells weakly transactivated a plasmid-borne metallothionein 1 (MT-1) promoter, but activated the expression of a marker gene controlled by an authentic HSV-1 promoter to a high level. In contrast, CMT-3 cells, which are CV-1 cells stably transformed with the simian virus 40 (SV40) large T-antigen (T-Ag) gene controlled by the MT-1 promoter, contained high levels of T-Ag following HSV-1 superinfection, but only if cells were preincubated in the presence of heavy-metal ions. This T-Ag was functional in that it could mediate the increase in copy number of a marker plasmid containing the SV40 origin of DNA replication. Pulse and continuous labeling of preinduced CMT-3 cells showed that T-Ag expression was not induced by HSV-1; but rather, HSV-1 superinfection resulted in the stabilization of pre-existing protein.
Blair
E D
ED
Department of Molecular Sciences, Wellcome Research Laboratories, Beckenham, United Kingdom.
Snowden
B W
BW
Wagner
E K
EK
D000818:Animals; D000957:Antigens, Viral, Tumor / Q000235:genetics* / Q000378:metabolism; D000998:Antiviral Agents / Q000494:pharmacology; D001087:Arabinonucleosides / Q000494:pharmacology; D002460:Cell Line; D004261:DNA Replication; D004279:DNA, Viral / Q000187:drug effects / Q000235:genetics*; D005455:Fluorescent Antibody Technique; D005784:Gene Amplification / Q000187:drug effects; D005786:Gene Expression Regulation; D005819:Genetic Markers; D008668:Metallothionein / Q000235:genetics; D008670:Metals / Q000494:pharmacology; D051379:Mice; D010957:Plasmids; D011233:Precipitin Tests; D011401:Promoter Regions, Genetic; D013539:Simian virus 40 / Q000235:genetics* / Q000502:physiology; D018139:Simplexvirus / Q000235:genetics*; D013936:Thymidine / Q000031:analogs & derivatives / Q000494:pharmacology; D014158:Transcription, Genetic; D014162:Transfection
D000957:Antigens, Viral, Tumor; D000998:Antiviral Agents; D001087:Arabinonucleosides; D004279:DNA, Viral; D005819:Genetic Markers; D008670:Metals; D008668:Metallothionein; C010032:thymine arabinoside; D013936:Thymidine