Expression of the v-erbA product, an altered nuclear hormone receptor, is sufficient to transform erythrocytic cells in vitro.
We investigated the effect of the v-erbA oncogene product, an altered thyroid hormone receptor, in chicken erythrocyte progenitor cells. Bone marrow cells were infected with a retrovirus vector (XJ12) carrying the v-erbA gene in association with the neoR gene. XJ12-infected erythrocyte progenitor cells gave rise to G418-resistant clones. Some were composed of blast cells identified as transformed CFU-Es blocked in their differentiation. These cells could be grown in culture for at least 25 generations and required anemic chicken serum as a source of erythropoietic growth factors. XJ12 can infect erythrocyte progenitor cells in vivo but is not sufficient to induce erythroleukemia. These data suggest that the activation of a nuclear hormone receptor might represent one step toward the development of neoplasms.
Gandrillon
O
O
Laboratoire de Biologie Moléculaire et Cellulaire UMR 13 CNRS, Ecole Normale Supérieure de Lyon, France.
Jurdic
P
P
Pain
B
B
Desbois
C
C
Madjar
J J
JJ
Moscovici
M G
MG
Moscovici
C
C
Samarut
J
J
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002645:Chickens; D003114:Colony-Forming Units Assay; D004912:Erythrocytes / Q000502:physiology*; D005786:Gene Expression Regulation; D006133:Growth Substances / Q000494:pharmacology; D066298:In Vitro Techniques; D018774:Oncogene Proteins v-erbA; D011956:Receptors, Cell Surface / Q000502:physiology*; D012191:Retroviridae Proteins / Q000235:genetics*
D000954:Antigens, Surface; D006133:Growth Substances; D018774:Oncogene Proteins v-erbA; D011956:Receptors, Cell Surface; D012191:Retroviridae Proteins