title: Transformation of NIH3T3 cells by A-MuLV proviral DNA: effect of plasmid linearization and carrier DNA on transformation efficiency.
abstract: We have optimized the conditions for efficient NIH3T3 focus formation by calcium phosphate transfection of proviral Abelson-murine leukemia virus (A-MuLV) plasmid DNA. Linearized pA-MuLV, P120 or P160 strains, when transfected with calf thymus carrier DNA, will produce 40-50 foci/100 ng pA-MuLV without co-transfecting Moloney-murine leukemia virus (Mo-MuLV) plasmid DNA.
authors.affiliation: Wistar Institute, Philadelphia, Pennsylvania 19104.
authors.forename: S K
authors.initials: SK
authors.lastname: Shore
authors.forename: E P
authors.initials: EP
authors.lastname: Reddy
mesh_terms: D000011:Abelson murine leukemia virus / Q000235:genetics*; D000818:Animals; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D004247:DNA / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D009052:Leukemia Virus, Murine / Q000235:genetics*; D051379:Mice; D008815:Mice, Inbred Strains; D010957:Plasmids; D011533:Proviruses / Q000235:genetics*; D012474:Salmon; D014162:Transfection
chemical_list: D004279:DNA, Viral; D004247:DNA