title: Effect of the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TTPA) upon membrane ionic exchanges in sea urchin eggs.
abstract: The effect of TPA (12-O-tetradecanoylphorbol-13-acetate) upon ionic exchanges was investigated in eggs of the sea urchin Arbacia lixula. Ouabain-sensitive 86Rb uptake and amiloride-sensitive 24Na influx were dramatically stimulated after TPA addition, indicating an enhancement of total ionic permeabilities. Stimulation by TPA of both Na+/H+ and Na+/K+ exchanges was canceled by amiloride, suggesting that activation of protein kinase C elicits, via Na+/H+ activity, stimulation of the sodium pump. However, TPA did not stimulate sodium pump activity and Na+/H+ exchange at the same rate as fertilization, probably because of an absence of calcium-dependent events. Further fertilization of TPA-pretreated eggs triggered an enhancement of sodium pump activity when the TPA treatment duration did not exceed 10 min. It is suggested that TPA activates preexisting transporting mechanisms in plasma membranes of unfertilized eggs (Na+ pump, Na+/H+ exchange) without eliciting corresponding regulatory mechanisms (Na+ stat, pH stat).
authors.affiliation: Laboratoire de Physiologie Cellulaire et Comparée, UA 651 CNRS, Université de Nice, France.
authors.forename: B
authors.initials: B
authors.lastname: Ciapa
authors.forename: D
authors.initials: D
authors.lastname: Allemand
authors.forename: P
authors.initials: P
authors.lastname: Payan
mesh_terms: D000584:Amiloride / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D000001:Calcimycin / Q000494:pharmacology; D002352:Carrier Proteins / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D010042:Ouabain / Q000494:pharmacology; D010063:Ovum / Q000187:drug effects / Q000378:metabolism*; D012413:Rubidium / Q000378:metabolism; D012617:Sea Urchins; D015398:Signal Transduction; D012964:Sodium / Q000378:metabolism; D017923:Sodium-Hydrogen Exchangers; D013084:Sperm-Ovum Interactions; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D015053:Zygote / Q000187:drug effects / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D017923:Sodium-Hydrogen Exchangers; C048793:methylisopropylamiloride; D000001:Calcimycin; D010042:Ouabain; D000584:Amiloride; D012964:Sodium; D012413:Rubidium; D013755:Tetradecanoylphorbol Acetate