Rapid diagnosis of tuberculosis by amplification of mycobacterial DNA in clinical samples.
A method based on DNA amplification and hybridisation for the rapid detection of Mycobacterium tuberculosis was used to test 35 clinical specimens (sputum, gastric aspirate, abscess aspirate, biopsy sample) from 34 patients in whom tuberculosis was suspected. M tuberculosis was detected in 15 specimens, 2 of which were negative by standard microbiological criteria (microscopy and/or culture). 20 specimens, negative by standard methods, were also negative by the amplification method. M tuberculosis was also detected in peripheral blood samples of 2 of 4 patients with AIDS from whom the organism had been isolated.
Brisson-Noël
A
A
Unité de Génie Microbiologique, Institut Pasteur, Paris.
Gicquel
B
B
Lecossier
D
D
Lévy-Frébault
V
V
Nassif
X
X
Hance
A J
AJ
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications*; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D005784:Gene Amplification; D006801:Humans; D015336:Molecular Probe Techniques; D009164:Mycobacterium Infections / Q000150:complications / Q000175:diagnosis* / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000235:genetics; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000235:genetics
D015342:DNA Probes; D004269:DNA, Bacterial