Characterization of Pseudomonas mercury-resistance transposon Tn502, which has a preferred insertion site in RP1.
Tn502mer differs in size and restriction map from the well-characterized Tn501mer. It also differs in its preferential and high-frequency insertion into the 6 kb PstI-C region of RP1. The affinity for this region is perpetuated in pVS76, a clone of RP1 PstI-C in pBR322. Restriction mapping of independent pVS76::Tn502 derivatives revealed that Tn502 inserted at the same site (or small region) in PstI-C corresponding to the 35 kb coordinate in RP1. Insertion occurred in both orientations, but one was preferred. When PstI-C was deleted from RP1, acquisition of Tn502 was reduced and the sites of insertion randomized.
Stanisich
V A
VA
Department of Microbiology, La Trobe University, Bundoora, Australia.
Arwas
R
R
Bennett
P M
PM
de la Cruz
F
F
D001665:Binding Sites; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D004352:Drug Resistance, Microbial / Q000235:genetics; D008628:Mercury / Q000494:pharmacology*; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics*; D015183:Restriction Mapping
D004251:DNA Transposable Elements; D008628:Mercury