title: Characterisation of human thyroid epithelial cells immortalised in vitro by simian virus 40 DNA transfection.
abstract: Human primary thyroid follicular epithelial cells were transfected with a plasmid containing an origin-defective SV40 genome (SVori-) to produce several immortal cell lines. Two of the 10 cell lines analysed expressed specific features of thyroid epithelial function (iodide-trapping and thyroglobulin production). These two lines were characterised in detail and found to be growth factor-independent, capable of anchorage-independent growth at low frequency but non-tumorigenic in nude mice. These differentiated, These differentiated, partially transformed cell lines were shown to be suitable for gene transfer at high frequency using simple coprecipitation techniques.
authors.affiliation: ICRF Molecular Oncology Group, Hammersmith Hospital, London, UK.
authors.forename: N R
authors.initials: NR
authors.lastname: Lemoine
authors.forename: E S
authors.initials: ES
authors.lastname: Mayall
authors.forename: T
authors.initials: T
authors.lastname: Jones
authors.forename: D
authors.initials: D
authors.lastname: Sheer
authors.forename: S
authors.initials: S
authors.lastname: McDermid
authors.forename: P
authors.initials: P
authors.lastname: Kendall-Taylor
authors.forename: D
authors.initials: D
authors.lastname: Wynford-Thomas
mesh_terms: D000328:Adult; D000818:Animals; D002460:Cell Line; D004279:DNA, Viral; D004848:Epithelium / Q000648:ultrastructure; D005260:Female; D005819:Genetic Markers; D006801:Humans; D007621:Karyotyping; D051379:Mice; D008819:Mice, Nude; D013539:Simian virus 40 / Q000235:genetics*; D012974:Sodium Iodide / Q000378:metabolism; D013954:Thyroglobulin / Q000032:analysis; D013961:Thyroid Gland / Q000378:metabolism / Q000648:ultrastructure; D014162:Transfection
chemical_list: D004279:DNA, Viral; D005819:Genetic Markers; D013954:Thyroglobulin; D012974:Sodium Iodide