Induction of aplastic anemia by intra-bone marrow inoculation of a molecularly cloned feline retrovirus.
Intra-bone marrow inoculation of cells infected with molecularly cloned feline retrovirus (FeLV-C-Sarma [FSC]) associated with aplastic anemia was examined to test the hypothesis that cell-to-cell transmission of virus might facilitate marrow cell infection and anemogenesis, a possibility suggested by in-vitro co-culture experiments. IBM inoculation of either FSC-infected feline marrow cells or fibroblasts of weanling cats bypassed age-related restriction of FSC replication, initiated viremia, caused irreversible depletion of erythroid burst forming units, and induced rapid fatal aplastic anemia. A second significant finding observed with FSC infection was pronounced systemic lymphoid depletion. The direct bone marrow inoculation system described facilitates experimental study of retrovirus-target cell interactions involved in erythroid aplasia.
Dornsife
R E
RE
Department of Pathology, Colorado State University, Fort Collins 80523.
Gasper
P W
PW
Mullins
J I
JI
Hoover
E A
EA
D000741:Anemia, Aplastic / Q000382:microbiology / Q000662:veterinary*; D000818:Animals; D001853:Bone Marrow / Q000382:microbiology; D002371:Cat Diseases / Q000382:microbiology*; D002415:Cats; D003001:Cloning, Molecular; D003114:Colony-Forming Units Assay; D006410:Hematopoiesis; D006801:Humans; D005256:Leukemia Virus, Feline / Q000472:pathogenicity*; D007958:Leukocyte Count; D008198:Lymph Nodes / Q000473:pathology; D008775:Methylprednisolone / Q000494:pharmacology
D008775:Methylprednisolone