Correlation between RsaI restriction fragment length polymorphism and electrophoretic types of human placental alkaline phosphatase.
Restriction fragment length polymorphism (RFLP) of human alkaline phosphatases was studied in a population sample from northern Sweden using a placental alkaline phosphatase (PLAP) cDNA probe. After digestion of human genomic DNA with RsaI the Southern blots showed DNA fragments most probably derived from three genes: PLAP, germ cell alkaline phosphatase (PLAP-like) and intestinal alkaline phosphatase. In agreement with a previous study, a two-allele polymorphism was found in PLAP with bands at 1.6 kilobases (A1) and 1.8 kilobases (A2). The gene frequencies of A1 and A2 were 0.46 and 0.54, respectively. There was a significant correlation between the RsaI RFLPs and electrophoretic types of PLAP; RSAI A2 showed an association with the ALP2p allele of PLAP.
Beckman
G
G
Department of Medical Genetics, University of Umeå, Sweden.
Beckman
L
L
Lundgren
E
E
Millán
J L
JL
Sikström
C
C
D000469:Alkaline Phosphatase / Q000235:genetics*; D000483:Alleles; D015139:Blotting, Southern; D015342:DNA Probes; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004592:Electrophoresis, Starch Gel; D005787:Gene Frequency; D006801:Humans; D009154:Mutation; D010641:Phenotype; D010920:Placenta / Q000201:enzymology*; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D013548:Sweden
D015342:DNA Probes; D015252:Deoxyribonucleases, Type II Site-Specific; C040158:GTAC-specific type II deoxyribonucleases; D000469:Alkaline Phosphatase