Naturally occurring serotype 2/subgroup II rotavirus reassortants in northern Brazil.
Nine serotype 2 human rotavirus strains were isolated in a community-based longitudinal study in Northern Brazil. Five of these strains had a 'long' RNA electrophoretic pattern and all five strains were determined to belong to subgroup II by ELISA assay, in contrast to properties common to serotype 2 human rotaviruses previously characterized. Hybridization studies of one of these unusual strains with 32P-labelled mRNAs derived from the prototype human strains Wa (serotype 1, subgroup II) and S2 (serotype 2, subgroup I) suggested that it was generated by a reassortment event in nature, in which a subgroup II, 'long' electropherotype rotavirus exchanged its serotype-specific gene and gene number 10 for the equivalent genes from a serotype 2, 'short' electropherotype virus.
Mascarenhas
J D
JD
Secao de Virologia do Instituto Evandro Chagas, FSESP, Para, Brasil.
Linhares
A C
AC
Gabbay
Y B
YB
de Freitas
R B
RB
Mendez
E
E
Lopez
S
S
Arias
C F
CF
D001938:Brazil; D006801:Humans; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012703:Serotyping
D012367:RNA, Viral