Kinetics of 3H-thymidine label in rat liver regenerating after partial hepatectomy and after damage with carbon tetrachloride or silica.
Rat liver DNA was labelled with [methyl-3H] thymidine after partial hepatectomy, carbon tetrachloride poisoning, or an intravenous injection of silica dust. Changes in DNA labelling were studied for 4 weeks after the single pulse. Total radioactivity incorporated into liver DNA after partial hepatectomy and after carbon tetrachloride administration remained on the same level when compared with that found after 1 h. DNA activity in liver of untreated rats and of rats treated with silica decreased by about 50% within the first 2 weeks and then remained on this level for the rest of the studied period. These differences may reflect the fact that hepatocytes that have a long life span are preferentially labelled in partially hepatectomized and CCl4-treated rats, while liver macrophages with a short half-life take up a large part of the label in intact rats and in rats treated with silica.
Kanta
J
J
Chmelar
V
V
D000818:Animals; D002251:Carbon Tetrachloride / Q000633:toxicity*; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D004391:Dust / Q000009:adverse effects; D005260:Female; D006498:Hepatectomy; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008115:Liver Regeneration; D051381:Rats; D011919:Rats, Inbred Strains; D012822:Silicon Dioxide / Q000009:adverse effects*; D013936:Thymidine / Q000378:metabolism*
D004391:Dust; D012822:Silicon Dioxide; D004247:DNA; D002251:Carbon Tetrachloride; D013936:Thymidine