Dual loci of DNA bending in the bovine papillomavirus upstream regulatory region.
Recombinant clones containing all or portions of the bovine papillomavirus (BPV) upstream regulatory region (URR) were constructed. When analyzed by polyacrylamide gel electrophoresis, the cloned URR sequences exhibited electrophoretic properties characteristic of DNA containing bend sites. Two distinct bend centers were identified, mapping to approximately 7477 and 7790 respectively on the BPV genome. No other loci of static DNA bending were detected in the URR region of the BPV genome.
Wilson
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Department of Medical Microbiology and Immunology, College of Medicine, Texas A & M University, College Station 77843.
D001483:Base Sequence; D052688:Bovine papillomavirus 1 / Q000235:genetics*; D003001:Cloning, Molecular; D004277:DNA, Single-Stranded / Q000235:genetics / Q000302:isolation & purification; D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D005809:Genes, Regulator; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D027383:Papillomaviridae / Q000235:genetics*
D004277:DNA, Single-Stranded; D004279:DNA, Viral