Detection of X-linked lymphoproliferative disease using molecular and immunovirologic markers.
OBJECTIVE
Detection of males affected with the X-linked lymphoproliferative disease (XLP) was sought using immunovirologic and molecular genetic linkage techniques. The study population consisted of 20 males in six families with XLP.


RESULTS
Concordance for detection of affected males was 100% when linkage analysis using DXS42 and DXS37 DNA probes and antibody responses to challenge with bacteriophage phi X174 were both determined. Most affected males showing IgG subclass immune deficiency could not produce antibodies to Epstein-Barr virus nuclear antigen and had deficient responses to challenge with bacteriophage phi X174.


CONCLUSIONS
Use of only one of the techniques described can fail to lead to the diagnosis of XLP, because problems can prevail with each individual determination.
Purtilo
D T
DT
Department of Pathology, University of Nebraska Medical Center, Omaha 68105-1065.
Grierson
H L
HL
Ochs
H
H
Skare
J
J
D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000096:biosynthesis; D010584:Bacteriophage phi X 174 / Q000276:immunology; D015415:Biomarkers; D002648:Child; D002675:Child, Preschool; D015342:DNA Probes; D008040:Genetic Linkage; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000145:classification; D007223:Infant; D008232:Lymphoproliferative Disorders / Q000175:diagnosis / Q000235:genetics*; D008297:Male; D012150:Polymorphism, Restriction Fragment Length; D014960:X Chromosome
D000914:Antibodies, Viral; D015415:Biomarkers; D015342:DNA Probes; D007074:Immunoglobulin G