The adeno-associated virus Rep78 gene inhibits cellular transformation induced by bovine papillomavirus.
Adeno-associated virus type 2 (AAV) is a helper dependent parvovirus which can inhibit the oncogenic and transforming potential of its helper viruses: adenoviruses (Ad) and herpesviruses. Here we have assayed AAV's ability to inhibit cellular transformation induced by bovine papillomavirus type 1 (BPV-1), a member of another family of DNA viruses. AAV was able to markedly inhibit BPV-1-induced transformation of contact-inhibited murine fibroblasts either by infection with virus particles or by DNA transfection. This inhibition was mapped to the full-length, unspliced, rep gene product, Rep78, of AAV. DNA replication by AAV or by BPV-1 was not required for inhibition of transformation to take place.
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Laboratory of Tumor Virus Biology, National Cancer Institute, Bethesda, Maryland 20892.
D000818:Animals; D052688:Bovine papillomavirus 1 / Q000235:genetics*; D002472:Cell Transformation, Viral; D004261:DNA Replication; D000229:Dependovirus / Q000235:genetics*; D005786:Gene Expression Regulation; D005796:Genes; D005814:Genes, Viral; D066298:In Vitro Techniques; D051379:Mice; D027383:Papillomaviridae / Q000235:genetics*; D010957:Plasmids