Involvement of sulfhydryl groups in the transport of L-tyrosine and L-tryptophan across the human red cell membrane in vitro.
In previous papers, we reported a deficit in tyrosine (TYR) and tryptophan (TRP) transport across the erythrocyte membrane in depressed patients. To investigate further the transport mechanism of the two precursors of monoamines, we tested in healthy subjects the role played by sulfhydryl groups (SH). These groups, cysteine residues, are localized on the intrinsic domain such as the transporters of chloride or sugars. We found that all sulfhydryl reagents that inactivated the SH induced a strong inhibition of the transport of amino acid across the red cell membrane when incubated in the plasma as medium. We concluded that a relationship might exist between these neutral amino acids and D-glucose transport.
Widmer
J
J
Service de la Recherche Biologique et de Psychopharmacologie Clinique, Institutions Universitaires de Psychiatrie de Genève, Suisse.
Gaillard
J M
JM
Bovier
P
P
Tissot
R
R
D002731:4-Chloromercuribenzenesulfonate / Q000494:pharmacology; D000328:Adult; D001693:Biological Transport, Active / Q000187:drug effects; D002478:Cells, Cultured; D004229:Dithiothreitol / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004910:Erythrocyte Membrane / Q000187:drug effects*; D005033:Ethylmaleimide / Q000494:pharmacology; D006801:Humans; D008623:Mercaptoethanol / Q000494:pharmacology; D008634:Mersalyl / Q000494:pharmacology; D008875:Middle Aged; D013438:Sulfhydryl Compounds / Q000494:pharmacology*; D014364:Tryptophan / Q000097:blood*; D014443:Tyrosine / Q000097:blood*
D013438:Sulfhydryl Compounds; D014443:Tyrosine; D008634:Mersalyl; D002731:4-Chloromercuribenzenesulfonate; D008623:Mercaptoethanol; D014364:Tryptophan; D005033:Ethylmaleimide; D004229:Dithiothreitol