Electron spin resonance detection of oxygen-centred radicals in murine macrophages stimulated with bacterial endotoxin.
The production of oxygen radicals by Bacille-Calmette-Guerin primed mouse macrophages stimulated with bacterial endotoxin has been investigated. Superoxide radicals were spin-trapped in this system with dimethylpyrroline-N-oxide after a lag period of 20-40 minutes. The electron spin resonance signals due to the superoxide radical adduct could be inhibited by superoxide dismutase but not by catalase.
Jackson
S K
SK
Department of Medical Microbiology, University of Wales College of Medicine, Health Park, Cardiff, U.K.
Stark
J M
JM
Rowlands
C C
CC
Evans
J C
JC
D000818:Animals; D004578:Electron Spin Resonance Spectroscopy; D004731:Endotoxins / Q000494:pharmacology*; D004926:Escherichia coli / Q000378:metabolism; D005260:Female; D005609:Free Radicals; D008262:Macrophage Activation; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D009163:Mycobacterium bovis / Q000378:metabolism*; D009580:Nitroblue Tetrazolium / Q000494:pharmacology; D010100:Oxygen / Q000032:analysis*; D013482:Superoxide Dismutase / Q000494:pharmacology
D004731:Endotoxins; D005609:Free Radicals; D009580:Nitroblue Tetrazolium; D013482:Superoxide Dismutase; D010100:Oxygen