86Rb(K) influx and [3H]ouabain binding by human platelets: evidence for beta-adrenergic stimulation of Na-K ATPase activity.
Although active transport of potassium into human platelets has been demonstrated previously, there is hitherto no evidence that human platelets have an ouabain-inhibitable Na-K ATPase in their membrane. The present study demonstrates active rubidium (used as an index of potassium influx), 86Rb(K), influx into platelets, inhibitable by ouabain, and also demonstrates the presence of specific [3H]ouabain binding by the human platelet. This 86Rb(K) influx was stimulated by adrenaline, isoprenaline, and salbutamol, but noradrenaline caused a mild inhibition. Active 86Rb(K) influx by platelets was inhibited markedly by timolol, mildly by atenolol, but not by phentolamine. Therefore, active 86Rb(K) influx in human platelets is enhanced by stimulation of beta adrenoceptors of the beta 2 subtype. The platelet may therefore replace the leukocyte in future studies of Na-K ATPase activity. This would be a considerable advantage in view of the ease and rapidity of preparation of platelets.
Turaihi
K
K
Department of Chemical Pathology and Human Metabolism, Royal Free Hospital and School of Medicine, London.
Khokher
M A
MA
Barradas
M A
MA
Mikhailidis
D P
DP
Dandona
P
P
D000328:Adult; D000420:Albuterol / Q000494:pharmacology; D001262:Atenolol / Q000494:pharmacology; D001693:Biological Transport, Active / Q000187:drug effects; D001792:Blood Platelets / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D004837:Epinephrine / Q000494:pharmacology; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology; D009638:Norepinephrine / Q000494:pharmacology; D010042:Ouabain / Q000097:blood* / Q000494:pharmacology; D010646:Phentolamine / Q000494:pharmacology; D011188:Potassium / Q000097:blood*; D011943:Receptors, Adrenergic, beta / Q000097:blood*; D012414:Rubidium Radioisotopes; D000254:Sodium-Potassium-Exchanging ATPase / Q000097:blood*; D013999:Timolol / Q000494:pharmacology; D014316:Tritium
D011943:Receptors, Adrenergic, beta; D012414:Rubidium Radioisotopes; D014316:Tritium; D001262:Atenolol; D010042:Ouabain; D013999:Timolol; D000254:Sodium-Potassium-Exchanging ATPase; D007545:Isoproterenol; D000420:Albuterol; D011188:Potassium; D009638:Norepinephrine; D004837:Epinephrine; D010646:Phentolamine