Effect of nerve growth factor on the synthesis of amino acids in PC12 cells.
Radioactive short-chain fatty acids preferentially label glutamine relative to glutamate in brain due to compartmentation of glutamine and glutamate. To determine whether this phenomenon occurs in a single cell culture model, we examined the effect of fatty acid chain length on the synthesis as well as pool size of selected amino acids in rat pheochromocytoma PC12 cells, a cell culture model of the large glutamate compartment in neurons. Intracellular 14C-amino acids were quantitated by HPLC, and the incorporation of [U-14C]-glucose, [1-14C]-butyrate, [1-14C]-octanoate, and [1-14C]-palmitate into five amino acids was measured in native and NGF-treated PC12 cells. NGF pretreatment decreased the intracellular concentration of amino acids as did addition of fatty acids but these effects were not additive. Specific activities of amino acids in native cells labelled by 14C-octanoate were 1,300 DPM/nmol, 490 DPM/nmol, 200 DPM/nmol, and 110 DPM/nmol for glutamate, aspartate, glutamine, and serine, respectively. No radioactivity was detected in alanine. Similar specific activities were noted when 14C-butyrate was the precursor; however, there was at least 5-fold less if 14C-palmitate was the precursor. Pretreatment of cells with NGF decreased the specific activity of amino acids by 25-65%. Specific activities of amino acids synthesized from 14C-glucose decreased in the following order: glutamate, 1,640 DPM/nmol; aspartate, 1,210 DPM/nmol; alanine, 580 DPM/nmol; glutamine, 275 DPM/nmol; and serine, 80 DPM/nmol for native cells. NGF pretreatment decreased the specific activities of glutamate and glutamine, but not of the other 3 amino acids. The preferred precursor for glutamate synthesis in native PC12 cells was glucose followed by octanoate, butyrate and palmitate (16:6:3:1).
Zielke
H R
HR
Department of Pediatrics, University of Maryland, Baltimore 21201.
Tildon
J T
JT
Kauffman
F C
FC
Baab
P J
PJ
D000310:Adrenal Gland Neoplasms / Q000378:metabolism*; D000596:Amino Acids / Q000096:biosynthesis*; D000818:Animals; D002087:Butyrates / Q000378:metabolism; D002250:Carbon Radioisotopes; D002460:Cell Line; D005947:Glucose / Q000378:metabolism; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D009414:Nerve Growth Factors / Q000494:pharmacology*; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D010673:Pheochromocytoma / Q000378:metabolism*; D011865:Radioisotope Dilution Technique
D000596:Amino Acids; D002087:Butyrates; D002250:Carbon Radioisotopes; D009414:Nerve Growth Factors; D010169:Palmitic Acids; D019308:Palmitic Acid; D005974:Glutamate-Ammonia Ligase; D005947:Glucose