Detection of P-glycoprotein in human leukemias using monoclonal antibodies.
Overexpression of a Mr 170,000 membrane glycoprotein (P-glycoprotein) is consistently associated with multidrug resistance in cell lines. Two monoclonal antibodies (Mab) against P-glycoprotein (265/F4 and C 219) were used to examine tumour samples from patients with leukemias for evidence of P-glycoprotein overexpression. High levels of P-glycoprotein (greater than 5% positive cells) were detected with both antibodies in samples from 3 out of 18 patients suggesting that a multidrug resistant phenotype may also occur in human leukemias.
Mattern
J
J
Department of Experimental Pathology, German Cancer Research Center, Heidelberg.
Efferth
T
T
Bak
M
M
Ho
A D
AD
Volm
M
M
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000911:Antibodies, Monoclonal; D001666:Binding Sites, Antibody; D004351:Drug Resistance / Q000235:genetics; D006801:Humans; D007938:Leukemia / Q000097:blood* / Q000188:drug therapy / Q000235:genetics; D008562:Membrane Glycoproteins / Q000097:blood* / Q000276:immunology; D009363:Neoplasm Proteins / Q000097:blood* / Q000276:immunology; D010641:Phenotype
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000911:Antibodies, Monoclonal; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins