Human monoclonal antibodies to influenza virus: IgG subclass and light chain distribution.
Three adults and three children were immunized with inactivated or live attenuated influenza vaccines and 98 IgG monoclonal antibodies derived from EBV immortalization of their blood lymphocytes were studied. All antibodies reacted specifically with influenza A H3N2 or H1N1 whole virus and 73 of 74 tested reacted with the purified HA glycoproteins. The majority (76%) of 77 monoclonal antibodies adequately tested by ELISA or solid phase RIA contained lambda light chains. ELISA analysis of the IgG subclass of the six persons' postimmunization anti-influenza serum activity and of monoclonal antibodies derived from them showed a similar predominance of IgG1.
Frank
A L
AL
Department of Pediatrics, University of Illinois College of Medicine, Chicago 60680.
Six
H R
HR
Marchini
A
A
D000328:Adult; D000911:Antibodies, Monoclonal / Q000145:classification*; D000914:Antibodies, Viral / Q000145:classification*; D001402:B-Lymphocytes / Q000276:immunology; D002472:Cell Transformation, Viral; D002999:Clone Cells; D004797:Enzyme-Linked Immunosorbent Assay; D004854:Herpesvirus 4, Human; D006801:Humans; D007074:Immunoglobulin G / Q000145:classification*; D007147:Immunoglobulin Light Chains / Q000145:classification*; D007223:Infant; D007231:Infant, Newborn; D009980:Influenza A virus / Q000276:immunology*; D014611:Vaccination
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007147:Immunoglobulin Light Chains