title: [ICO-10 monoclonal antibodies to the Thy-1 antigen].
abstract: Mouse monoclonal antibodies (MAB) ICO-10 to Thy-1 antigen were obtained. MAB ICO-10 reacted in indirect immunofluorescence test with 5.7 +/- 0.8% human thymocytes. Antibodies did not react with granulocytes, monocytes, T- and non-T cells from peripheral blood, and with marrow cells of healthy donors. MAB ICO-10 reacted with blast cells from 25 of 53 patients with T-cell acute lymphoblastic leukemia (ALL), from 2 of 5 patients with B-cell ALL. This antigen was absent on blood and marrow cells from some patients with ALL, 80 patients with chronic lymphoid leukemia, 54 patients with chronic granulocytic leukemia at the stage of blastic crisis, 128 patients with acute nonlymphoblastic leukemia. Antibodies are specifically bound to thymocytes and spleen cells of Thy 1.1 and Thy 1.2 mice. MAB ICO-10 detect Thy-1 antigen expressed on human hematopoietic cells. MAB ICO-10 may be applied for human leukemia and lymphoma immune diagnosis.
authors.forename: O V
authors.initials: OV
authors.lastname: Korotkova
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Baryshnikov
authors.forename: N N
authors.initials: NN
authors.lastname: Tupitsyn
authors.forename: K L
authors.initials: KL
authors.lastname: Chimishkian
authors.forename: V N
authors.initials: VN
authors.lastname: Kostrykina
mesh_terms: D000293:Adolescent; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000302:isolation & purification*; D000937:Antigen-Antibody Reactions; D000954:Antigens, Surface / Q000276:immunology*; D001773:Blood Cells / Q000276:immunology; D001853:Bone Marrow / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D006801:Humans; D007114:Immunization; D007223:Infant; D007938:Leukemia / Q000276:immunology; D008228:Lymphoma, Non-Hodgkin / Q000276:immunology; D051379:Mice; D013045:Species Specificity; D018800:Thy-1 Antigens; D013950:Thymus Gland / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D018800:Thy-1 Antigens
vernacular_title: Monoklonal'nye antitela IKO-10 k antigenu Thy-1.