Detection and characterization of the fibroblast growth factor-related oncoprotein INT-2.
Products of the fibroblast growth factor-related proto-oncogene int-2 have been detected by using a monoclonal antibody and polyclonal antisera raised against synthetic peptides predicted from the DNA sequence. COS-1 monkey cells transfected with int-2 DNA linked to the simian virus 40 early promoter contained at least four int-2-specific proteins, presumably representing modified forms of the expected 27-kilodalton primary translation product. The level of expression was increased approximately six- to eightfold by mutation of sequences around the presumed initiation codon, negating their capacity to encode a short oligopeptide in the +1 reading frame. Both tunicamycin inhibition and in vitro translation experiments indicated that some of the modifications correspond to asparagine-linked glycosylation, for which the sequence predicts a single site. In line with the similarities between INT-2 and other fibroblast growth factors, the in vitro translation products functioned as weak mitogens for mammary epithelial cells.
Dixon
M
M
Imperial Cancer Research Fund Laboratories, Lincoln's Inn Fields, London, United Kingdom.
Deed
R
R
Acland
P
P
Moore
R
R
Whyte
A
A
Peters
G
G
Dickson
C
C
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis / Q000235:genetics; D005346:Fibroblast Growth Factors; D005786:Gene Expression Regulation; D007106:Immune Sera; D008969:Molecular Sequence Data; D009154:Mutation; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D011518:Proto-Oncogene Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology / Q000494:pharmacology; D013539:Simian virus 40 / Q000235:genetics; D014162:Transfection; D014415:Tunicamycin / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D007106:Immune Sera; D011518:Proto-Oncogene Proteins; D014415:Tunicamycin; D005346:Fibroblast Growth Factors; D004247:DNA