Expression of surface antigen and mRNA for the CD11c (alpha X, p150) subunit of the human leukocyte adherence receptor family in hematopoietic cells.
We characterized the surface antigen and mRNA expression for the CD11c (alpha X, p150) subunit of the human leukocyte adherence receptor family during hematopoietic cell differentiation. The CD11c subunit antigen and mRNA are constitutively expressed in undifferentiated HL-60 promyelocytic leukemia cells, and levels increase markedly with differentiation along the monocyte/macrophage pathway using phorbol myristate acetate. Human monocyte-derived macrophages and human alveolar macrophages express elevated levels of the CD11c subunit antigen and mRNA, indicating that the changes observed in vitro are present in vivo. Dot blot analysis of immature and mature lymphoid and myeloid cells and cell lines demonstrate equivalent levels of CD11c mRNA expression. We conclude that CD11c gene expression is selectively increased during hematopoietic cell differentiation along the monocyte/macrophage pathway.
Dudley
D
D
Medical Research Division, Seattle Veterans Administration Medical Center, WA 98108.
Baker
D M
DM
Hickey
M J
MJ
Hickstein
D D
DD
D000943:Antigens, Differentiation / Q000235:genetics*; D018845:CD11 Antigens; D002448:Cell Adhesion; D002454:Cell Differentiation; D002460:Cell Line; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000276:immunology; D015473:Leukemia, Promyelocytic, Acute / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D009000:Monocytes / Q000276:immunology; D009693:Nucleic Acid Hybridization; D011650:Pulmonary Alveoli / Q000166:cytology; D012333:RNA, Messenger / Q000235:genetics*; D014407:Tumor Cells, Cultured
D000943:Antigens, Differentiation; D018845:CD11 Antigens; D012333:RNA, Messenger