title: Sanded nuclei in delta patients.
abstract: Sanded nuclei are nuclei with eosinophilic inclusions identified by light microscopy in cases of chronic hepatitis B virus infection. In hematoxylin and eosin-stained sections, these inclusions have an almost homogeneous, finely granular texture giving a sandy appearance. They have been related to excess hepatitis B core antigen formation. We have studied liver biopsies from two HBsAg positive immunosuppressed patients with numerous sanded nuclei, morphologically identical to those previously described in hepatitis B. Immunohistochemically, sanded nuclei showed a strong nuclear positivity for delta antigen, but were negative for hepatitis B core antigen. Hepatitis B core particles were not demonstrable by electron microscopy. To our knowledge, this is the first time that sanded nuclei have been related to hepatitis delta virus (HDV) infection.
authors.affiliation: Hospital Ramón y Cajal, Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Moreno
authors.forename: S
authors.initials: S
authors.lastname: Ramón y Cajal
authors.forename: M
authors.initials: M
authors.lastname: Marazuela
authors.forename: V
authors.initials: V
authors.lastname: Carreño
authors.forename: J M
authors.initials: JM
authors.lastname: Milicua
authors.forename: E
authors.initials: E
authors.lastname: Cerezo
authors.forename: C
authors.initials: C
authors.lastname: Cuesta
authors.forename: H
authors.initials: H
authors.lastname: Oliva
mesh_terms: D000328:Adult; D000956:Antigens, Viral / Q000032:analysis; D002467:Cell Nucleus / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006509:Hepatitis B / Q000473:pathology; D006512:Hepatitis B Core Antigens / Q000032:analysis; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D003699:Hepatitis D / Q000473:pathology*; D003698:Hepatitis Delta Virus / Q000276:immunology; D035923:Hepatitis delta Antigens; D006801:Humans; D002479:Inclusion Bodies / Q000648:ultrastructure; D008099:Liver / Q000648:ultrastructure*; D008297:Male; D008854:Microscopy, Electron
chemical_list: D000956:Antigens, Viral; D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens; D035923:Hepatitis delta Antigens; C442244:hepatitis delta virus large antigen