Protective effect of a low-dose of cyclophosphamide in experimental infection of guinea pigs with Junin virus.
Administration of cyclophosphamide (CY) to guinea pigs infected with a lethal strain of Junin virus (JV) delayed the time of death, with survival of a small number of animals. Virological studies showed a temporary decrease of virus concentration in blood and viscera shortly after the CY injection. In the pathological study no differences were found in the organic lesions present in CY-treated and nontreated animals, with the exception of the pulmonary alterations. In CY-treated guinea pigs the lungs appeared almost normal, but in the control, nontreated animals severe alterations with the pattern of the "respiratory distress syndrome of the adult" were consistently present. In in vitro experiments, incorporation of serum collected from guinea pigs injected 30 minutes before exsanguination with CY to cell cultures, infected with JV, prevented virus replication. On the basis of these results it is suggested that the delay of time of death and eventual survival of CY-treated guinea pigs after JV infection depends on a direct antiviral effect of the drug rather than on its known immunosuppressive action. In addition, the absence of pulmonary alterations in CY-treated animals was tentatively considered to be dependent on the marked polymorphonuclear leukocyte depletion induced by the drug.
Ponzinibbio
C
C
Department of Pathology, School of Medicine, La Plata University, Argentina.
González
P
P
Laguens
R P
RP
D000818:Animals; D001116:Arenaviridae / Q000187:drug effects*; D006477:Arenaviruses, New World / Q000187:drug effects* / Q000254:growth & development; D002470:Cell Survival; D002478:Cells, Cultured; D003520:Cyclophosphamide / Q000627:therapeutic use*; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D006478:Hemorrhagic Fever, American / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D009928:Organ Specificity; D010948:Viral Plaque Assay
D003520:Cyclophosphamide