Specific desensitization of the epidermal growth factor receptor by pp60v-src.
BALB/c 3T3 cells infected with a temperature-sensitive mutant (LA90) of RSV have been used to investigate possible heterologous interactions between the pp60v-src tyrosyl kinase and the epidermal growth factor (EGF) and bradykinin receptors. The LA90 pp60v-src exhibits a very rapid activation t1/2 (less than 5 min) of protein kinase activity on decreasing the temperature from 40 degrees C to 35 degrees C. This change in temperature was also found to induce a very rapid decrease in the affinity for 125I-EGF of receptors on the RSV-LA90-infected cells but not of those on control parental cells. However, no significant changes were detected in the binding of 3H-bradykinin to either cell line. Two separable processes control the desensitization of the EGF receptor by pp60v-src, both of which are independent of protein kinase C. The first is rapid and transient, while the second is sensitive to cycloheximide and persists long after inactivation of pp60v-src.
Gray
G M
GM
Division of Toxicology, Environmental Health Sciences Center, University of Rochester Medical Center, New York 14642.
Wei
C N
CN
Macara
I G
IG
D000818:Animals; D001920:Bradykinin / Q000493:pharmacokinetics; D002461:Cell Line, Transformed; D003513:Cycloheximide / Q000494:pharmacology; D004789:Enzyme Activation; D066246:ErbB Receptors / Q000187:drug effects / Q000378:metabolism*; D015688:Oncogene Protein pp60(v-src); D010641:Phenotype; D010703:Phorbol Esters / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism; D018002:Receptors, Bradykinin; D017981:Receptors, Neurotransmitter / Q000378:metabolism; D012191:Retroviridae Proteins / Q000378:metabolism*; D013696:Temperature
D010703:Phorbol Esters; D018002:Receptors, Bradykinin; D017981:Receptors, Neurotransmitter; D012191:Retroviridae Proteins; D003513:Cycloheximide; D066246:ErbB Receptors; D015688:Oncogene Protein pp60(v-src); D011493:Protein Kinase C; D001920:Bradykinin