The influence of adsorption of native and modified antibodies on their activity.
Immobilization of biomolecules to solid phase materials has been widely used in many areas (e.g., purification, analytical chemistry, and catalysis). The interfacial properties of immobilized antibodies on pretreated silica and hydrogel surfaces were explored by comparing native and modified antibodies with respect to their surface activity. The antibody was modified by exposing it to a low pH solution prior to immobilization. Both physical adsorption and covalent immobilization methods were studied. It was found that the surface activity of the modified antibody is higher than that of the native antibody on two silica surfaces. The results of this study demonstrate that the adsorption properties of the antibodies play an important role in their covalent immobilization on certain types of solid supports.
Lin
J N
JN
Department of Bioengineering, College of Engineering, University of Utah, Salt Lake City 84112.
Andrade
J D
JD
Chang
I N
IN
D000327:Adsorption; D000906:Antibodies / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D006863:Hydrogen-Ion Concentration; D011489:Protein Denaturation; D012685:Sepharose; D012709:Serum Albumin / Q000276:immunology; D012822:Silicon Dioxide; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship
D000906:Antibodies; D012709:Serum Albumin; D012822:Silicon Dioxide; D012685:Sepharose