The structure of tyrosine aminotransferase. Evidence for domains involved in catalysis and enzyme turnover.
The primary structure of tyrosine aminotransferase, as deduced from the nucleotide sequence of complementary DNA, was confirmed by fast atom bombardment mass spectrometry of tryptic peptides derived from the purified protein. Limited digestion of the native enzyme with trypsin released an acetylated, amino-terminal peptide; the new amino terminus in the modified enzyme was Val65. Endogenous proteases generated a chromatographically separable form of tyrosine aminotransferase that began at Lys35. Neither trypsin nor the other proteases altered the catalytic activity of tyrosine aminotransferase. Reduction of the holoenzyme with sodium borohydride yielded a major tryptic peptide containing phosphopyridoxamine bound to lysine 280, which probably functions in transamination. The carboxyl terminus of tyrosine aminotransferase contains features that typify proteins with short half-lives; it includes two negatively charged, hydrophilic segments that are enriched for glutamyl residues and are similar to a PEST region in ornithine decarboxylase (Rogers, S., Wells, R., and Rechsteiner, M. (1986) Science 234, 364-368). Tyrosine aminotransferase belongs to a superfamily of enzymes which includes aspartate aminotransferase and can be aligned so that many invariant, functional residues coincide. Like the isoenzymes of aspartate aminotransferase, tyrosine aminotransferase may contain two domains, with a central, catalytic core, and a small domain made up of both amino- and carboxyl-terminal components. We speculate that the exposed small domain may confer the unusually rapid degradative rate that characterizes this enzyme.
Hargrove
J L
JL
Department of Anatomy and Cell Biology, Emory University School of Medicine, Atlanta, Georgia 30322.
Scoble
H A
HA
Mathews
W R
WR
Baumstark
B R
BR
Biemann
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D011487:Protein Conformation; D051381:Rats; D011919:Rats, Inbred Strains; D014444:Tyrosine Transaminase / Q000235:genetics / Q000378:metabolism*
D004247:DNA; D014444:Tyrosine Transaminase