Radiation-induced DNA damage and repair in quiescent and proliferating human tumor cells in vitro.
The purpose of this study was to examine radiation-induced DNA strand breakage and repair in quiescent and proliferating human tumor cells in vitro and determine their relationship to radiation sensitivity and potentially lethal damage repair (PLDR). Using centrifugal elutriation we have isolated from fed plateau-phase cultures of HEp-3 human squamous carcinoma cells, relatively pure populations of quiescent and proliferating cells. This was confirmed by both [3H]-thymidine labelling and acridine orange (AO) staining with flow cytometry. Quiescent cells were more sensitive to ionizing radiation (Do = 0.97 Gy) than were proliferating cells (Do = 1.28 Gy). However, quiescent cells showed higher repair of potentially lethal damage (PLDR) than did proliferating cells. Repair of single-strand breaks (ssb) and double-strand breaks (dsb) as measured by filter elution did not differ significantly between quiescent and proliferating cells. For both populations, ssb and dsb repair kinetics and final damage remaining were the same, suggesting that repair of DNA strand breaks is not entirely responsible for the difference in radiation sensitivity between quiescent and proliferating cells.
Wilson
K M
KM
University of Rochester Cancer Center, NY 14642.
Keng
P C
PC
D002294:Carcinoma, Squamous Cell / Q000473:pathology; D002455:Cell Division; D002460:Cell Line; D004249:DNA Damage; D004260:DNA Repair; D004273:DNA, Neoplasm / Q000528:radiation effects*; D004277:DNA, Single-Stranded / Q000528:radiation effects; D006801:Humans; D066298:In Vitro Techniques; D011836:Radiation Tolerance
D004273:DNA, Neoplasm; D004277:DNA, Single-Stranded