Interactions of human lymphoblasts with targeted vesicles containing Sendai virus envelope proteins.
We have studied the internalization of targeted fusogenic liposome content to leukemic T cells (CEM) in vitro. We describe a method for the covalent coupling of T101 antibody to the surface of liposomes and the incorporation of fusogenic viral protein into the liposome membrane. Hygromycin B, an impermeant inhibitor of protein synthesis, was encapsulated in the targeted fusogenic liposomes and delivered directly to the cytoplasm of leukemic T cells by fusion between the two membranes. The cytotoxic effect was measured by [3H]thymidine incorporation. We show that CEM are rapidly and specifically killed by the drug encapsulated in the targeted fusogenic liposomes. This effect is due to the binding of the liposome by means of the antibody and then to the fusion of the liposome with the targeted cell membrane, mediated by F protein.
Sechoy
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O
U.A. CNRS 530, INSERM U 58, Montpellier, France.
Vidal
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M
Philippot
J R
JR
Bienvenue
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D000906:Antibodies / Q000276:immunology / Q000378:metabolism; D000954:Antigens, Surface / Q000032:analysis / Q000276:immunology; D002462:Cell Membrane / Q000032:analysis / Q000378:metabolism; D006801:Humans; D006921:Hygromycin B / Q000032:analysis / Q000378:metabolism; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000378:metabolism / Q000473:pathology*; D008081:Liposomes / Q000032:analysis / Q000378:metabolism; D010222:Parainfluenza Virus 1, Human / Q000032:analysis*; D014759:Viral Envelope Proteins / Q000032:analysis / Q000276:immunology / Q000378:metabolism*
D000906:Antibodies; D000954:Antigens, Surface; D008081:Liposomes; D014759:Viral Envelope Proteins; D006921:Hygromycin B