Blockage of morphological transformation of chick embryo fibroblasts infected with Rous sarcoma virus by N-myristoyl glycinal diethylacetal in vitro.
N-Myristoyl glycinal diethylacetal strongly inhibited morphological transformation of chick embryo fibroblasts infected with a temperature-sensitive mutant (tsNY68) of Rous sarcoma virus. Myristoylated or nonmyristoylated pp60v-src, which were expressed in tsNY68-infected cells in the absence or presence of the compound, were identified separately by fluorography or immunoblotting analysis after sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the [3H]myristate-labeled cell lysate. The results taken together suggest that the blockage of morphological transformation was caused by prevention of protein myristoylation of the transforming protein pp60v-src.
Shoji
S
S
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
Matsunaga
M
M
Tsujita
R
R
Kubota
Y
Y
D000079:Acetaldehyde / Q000494:pharmacology*; D000080:Acetals / Q000494:pharmacology*; D000818:Animals; D001358:Avian Sarcoma Viruses / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D002472:Cell Transformation, Viral / Q000187:drug effects*; D002642:Chick Embryo; D005347:Fibroblasts / Q000382:microbiology / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D009154:Mutation; D015688:Oncogene Protein pp60(v-src); D012191:Retroviridae Proteins / Q000378:metabolism
D000080:Acetals; D012191:Retroviridae Proteins; C057591:N-myristoyl glycinal diethylacetal; D015688:Oncogene Protein pp60(v-src); D000079:Acetaldehyde