Spin label studies of erythrocytes during storage of blood.
The kinetic behavior of the spin label MAL-6 in the interaction with differently aged human erythrocyte membranes was evaluated by monitoring the rate of disappearance of the room temperature ESR signal due to the MAL-6 spin label added to blood after storage at 4 degrees C or after incubation of red cells at 37 degrees C in a protein-free medium. After 35 days of blood storage or 60 h of erythrocytes incubation at 37 degrees C the decrease of the intensity of the MAL-6 ESR spectra in respect to control samples is markedly enhanced and the correspondent kinetic constants significantly increase. Signal decay of MAL-6 is a further proof that during storage of blood under blood bank conditions or during an artificial ageing of erythrocytes at 37 degrees C, profound modifications occur in the human erythrocyte membrane.
Pessina
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Dipartimento di Chimica, Universita degli Studi di Siena, Italy.
Ciccoli
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Picchi
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MP
Laschi
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Fanetti
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G
D001793:Blood Preservation; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D013997:Time Factors