Effects of islet hormones on insulin secretion from cloned B cell lines, HIT-T15 and RINm5F.
The effects of the islet cell hormones glucagon, somatostatin-28 and pancreatic polypeptide on insulin secretion from cultured cloned pancreatic B cells (HIT-T15 and RINm5F) have been investigated. Glucagon stimulates the secretion of insulin from HIT-T15 cells in the absence and presence of glucose and from RINm5F cells in the absence and presence of glyceraldehyde. HIT-T15 cells were more sensitive to the stimulatory effect of glucagon than RINm5F cells. Somatostatin-28 and pancreatic polypeptide, both alone and in combination, reduced glucose- and glucagon-stimulated insulin release from HIT-T15 cells and glyceraldehyde- and glucagon-stimulated insulin release from RINm5F cells. HIT-T15 cells were more sensitive to the inhibitory actions of somatostatin-28 and pancreatic polypeptide than RINm5F cells. This study supports the hypothesis that insulin release from normal B cells may be modified by the paracrine activity of islet hormones, glucagon, somatostatin and pancreatic polypeptide and probably occurs before any fine tuning imposed by subsequently released insulin.
Lambert
D G
DG
Pharmaceutical Sciences Institute, Aston University, Birmingham.
Atkins
T W
TW
D000818:Animals; D002460:Cell Line; D002999:Clone Cells / Q000187:drug effects; D005934:Glucagon / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects*; D010191:Pancreatic Polypeptide / Q000494:pharmacology*; D013004:Somatostatin / Q000494:pharmacology*; D053561:Somatostatin-28
D007328:Insulin; D013004:Somatostatin; D010191:Pancreatic Polypeptide; D053561:Somatostatin-28; D005934:Glucagon