title: Establishment of a cell line producing bone morphogenetic protein from a human osteosarcoma.
abstract: A human osteosarcoma cell line was established from a biopsy specimen from a 13-year-old girl. The osteosarcoma tissue was maintained in athymic nude mice (Balb C nu/nu) by serial transplantation for three years. The tumor was excised from a host mouse and digested with collagenase. The isolated cells were cultured by 98 passages in 14 months, and clones of osteosarcoma cells were obtained by limiting dilution. A clone named human osteosarcoma cell 6 (H-OS-6) that showed the osteoblastic phenotypes of productions of bone morphogenetic protein (BMP) and alkaline phosphatase and a response to human parathyroid hormone (h-PTH 1-34) was selected. The morphology of its chromosomes indicated its human origin. This human osteosarcoma cell line is unique in producing BMP under in vitro conditions.
authors.affiliation: Department of Orthopaedic Surgery, Osaka University Medical School, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Takaoka
authors.forename: H
authors.initials: H
authors.lastname: Yoshikawa
authors.forename: K
authors.initials: K
authors.lastname: Masuhara
authors.forename: K
authors.initials: K
authors.lastname: Sugamoto
authors.forename: T
authors.initials: T
authors.lastname: Tsuda
authors.forename: Y
authors.initials: Y
authors.lastname: Aoki
authors.forename: K
authors.initials: K
authors.lastname: Ono
authors.forename: Y
authors.initials: Y
authors.lastname: Sakamoto
mesh_terms: D000293:Adolescent; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D019485:Bone Morphogenetic Proteins; D002999:Clone Cells / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D005260:Female; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D009368:Neoplasm Transplantation; D012516:Osteosarcoma / Q000378:metabolism*; D010281:Parathyroid Hormone / Q000494:pharmacology; D014176:Protein Biosynthesis; D014407:Tumor Cells, Cultured / Q000378:metabolism
chemical_list: D019485:Bone Morphogenetic Proteins; D009363:Neoplasm Proteins; D010281:Parathyroid Hormone; D000242:Cyclic AMP; D000469:Alkaline Phosphatase