Glutathione metabolism at the blood-cerebrospinal fluid barrier.
Glutathione metabolism and transport in the choroid plexus were probed by determining the effects of administration to rats of several compounds (buthionine sulfoximine, L-2-oxothiazolidine-4-carboxylate, L-(alpha 5,5S)-alpha-amino-3-chloro-4,5-dihydro-5-isoxazole acetic acid, gamma-glutamyl alanine, and glutathione monoethyl ester) on the levels of glutathione and cysteine in the cerebrospinal fluid. The findings indicate that glutathione is actively metabolized in the choroid plexus by pathways similar to those in kidney and other tissues. The level of glutathione in the cerebrospinal fluid can be decreased or increased by giving compounds that do not, under similar conditions, appreciably alter total brain levels of glutathione. Glutathione monoethyl ester is effectively transported into the cerebrospinal fluid.
Anderson
M E
ME
Department of Biochemistry, Cornell University Medical College, New York, New York 10021.
Underwood
M
M
Bridges
R J
RJ
Meister
A
A
D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D001812:Blood-Brain Barrier / Q000502:physiology*; D019328:Buthionine Sulfoximine; D002831:Choroid Plexus / Q000187:drug effects / Q000378:metabolism; D003545:Cysteine / Q000134:cerebrospinal fluid; D004151:Dipeptides / Q000494:pharmacology*; D005978:Glutathione / Q000134:cerebrospinal fluid* / Q000378:metabolism / Q000494:pharmacology / Q000031:analogs & derivatives; D007555:Isoxazoles / Q000494:pharmacology; D008297:Male; D008717:Methionine Sulfoximine / Q000494:pharmacology; D011761:Pyrrolidonecarboxylic Acid; D051381:Rats; D011919:Rats, Inbred Strains; D013844:Thiazoles / Q000494:pharmacology; D053778:Thiazolidines; D005723:gamma-Glutamyltransferase / Q000037:antagonists & inhibitors
D000963:Antimetabolites; D004151:Dipeptides; D007555:Isoxazoles; D013844:Thiazoles; D053778:Thiazolidines; D008717:Methionine Sulfoximine; D019328:Buthionine Sulfoximine; C061654:gamma-glutamylalanine; C042431:S-ethyl glutathione; D005723:gamma-Glutamyltransferase; D005978:Glutathione; D003545:Cysteine; C006303:acivicin; D011761:Pyrrolidonecarboxylic Acid; C029536:2-oxothiazolidine-4-carboxylic acid