Demonstration of different regional distributions of calcineurin subunits using monoclonal antibodies.
Immunohistochemical localizations of calcineurin subunits A (60 KDa) and B (20 KDa) were examined in a rat brain using subunit specific monoclonal antibodies. The immunoreactivity of the subunit A was abundant in the hippocampus, in the striatum, and in the thalamus, but weak in the neocortex. On the contrary, the immunoreactivity of the subunit B was more abundant in the cortex, and it was more ubiquitous than subunit A. The distribution of subunit A in the rat brain very well agreed with that of zinc, which is an intrinsic metal ion and a potent inhibitor of calcineurin phosphatase.
Matsui
H
H
Dept. of Physiology Kagawa Med. Sch., Japan.
Itano
T
T
Etoh
S
S
Tokuda
M
M
Wang
J H
JH
Hatase
O
O
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001923:Brain Chemistry; D019703:Calcineurin; D002148:Calmodulin-Binding Proteins / Q000032:analysis*; D015151:Immunoblotting; D010749:Phosphoprotein Phosphatases / Q000032:analysis*; D011487:Protein Conformation; D051381:Rats
D000911:Antibodies, Monoclonal; D002148:Calmodulin-Binding Proteins; D019703:Calcineurin; D010749:Phosphoprotein Phosphatases