Identification of a disulfide between cysteine 214 and cysteine 277 in the beta subunit of native (Na+ + K+)ATPase.
Two peptides, produced during tryptic digestion and thermolytic digestion, respectively, and containing the same intact disulfide from the beta polypeptide of (Na+ + K+)ATPase from Torpedo californica, were isolated and unambiguously identified. The disulfide is between Cysteine 214 and Cysteine 277.
Kellaris
K V
KV
Department of Chemistry, University of California, San Diego, La Jolla 92093.
D000595:Amino Acid Sequence; D000818:Animals; D003545:Cysteine / Q000302:isolation & purification*; D004220:Disulfides / Q000302:isolation & purification*; D006868:Hydrolysis; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification; D011950:Receptors, Cholinergic / Q000032:analysis; D000254:Sodium-Potassium-Exchanging ATPase; D014101:Torpedo; D014357:Trypsin
D004220:Disulfides; D010446:Peptide Fragments; D011950:Receptors, Cholinergic; D014357:Trypsin; D000254:Sodium-Potassium-Exchanging ATPase; D003545:Cysteine