Hemagglutination with pseudorabies virus. Brief report.
Pseudorabies virus grown in CPK cell cultures was tested for hemagglutination (HA) with erythrocytes of a variety of species at 4 degrees C, 25 degrees C and 37 degrees C. HA was observed at all temperatures with mouse erythrocytes but not with cattle, sheep, goat, swine, cat, rabbit, guinea pig, rat, mongolian gerbil, chicken, and goose erythrocytes. Mice showed a strain variation in agglutinability of their erythrocytes, requiring selection of mice to obtain erythrocytes for HA. The HA reaction was inhibited by specific antiserum. Some factors involved in the HA and HA-inhibition (HI) were investigated and standard HA and HI tests were established. HI antibody titers of individual pig sera showed a significant positive correlation with their neutralizing antibody titers.
Tetsu
N
N
Department of Veterinary Epizootiology, College of Agriculture and Veterinary Medicine, Nihon University, Kanagawa, Japan.
Inaba
Y
Y
Yukawa
M
M
Yoshiki
K
K
Hirahara
T
T
Furuya
Y
Y
Ito
S
S
Yonemochi
A
A
Ishikawa
H
H
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D002417:Cattle; D005777:Geese; D005849:Gerbillinae; D006041:Goats; D006168:Guinea Pigs; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D011557:Pseudorabies / Q000175:diagnosis; D011817:Rabbits; D051381:Rats; D012756:Sheep; D013045:Species Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000382:microbiology
D000956:Antigens, Viral