Genetic relatedness of the Kemerovo serogroup viruses: III. RNA-RNA blot hybridization and gene reassortment in vitro of the Chenuda serocomplex.
The dsRNA polyacrylamide gel profiles of five Chenuda serocomplex viruses were distinct. Blot hybridization and gene reassortment in vitro studies demonstrated that the Chenuda serocomplex may be divided into three sets: Chenuda, Huacho, and Mono Lake. Genes were highly conserved among members of each set, whereas genes were not highly conserved between members of different sets. Gene reassortment was demonstrated in intra-set crosses, but inter-set crosses did not yield reassortant progeny. The taxonomic significance of these data to the Chenuda serocomplex is discussed.
Brown
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SE
Department of Entomology, Colorado State University, College of Agricultural Sciences, Fort Collins 80523.
Morrison
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HG
Knudson
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D004591:Electrophoresis, Polyacrylamide Gel; D015321:Gene Rearrangement; D009693:Nucleic Acid Hybridization; D012330:RNA, Double-Stranded / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis*; D012087:Reoviridae / Q000145:classification / Q000235:genetics*; D012703:Serotyping
D012330:RNA, Double-Stranded; D012367:RNA, Viral