Antibodies to an NH2-terminal myristoyl glycine moiety can detect NH2-terminal myristoylated proteins in the retrovirus-infected cells.
Novel antibodies were raised against a synthetic NH2-terminal myristoyl glycine moiety which is characteristic of N-myristoyl-proteins. Antisera raised against N-myristoyl-Gly-hemocyanin reacted with N-myristoyl-Gly-[125I]albumin. The immunoreaction was competed for by albumin conjugated with N-myristoyl-glycine, while underivatized albumin had no effect. Of the [3H]myristate-labeled proteins detected, pp60v-src, which is a transforming protein of Rous sarcoma virus, and p19gag and p17gag, which are core proteins in the human T-cell leukemia virus and the human immunodeficiency virus, were identified as N-myristoylated proteins by the radioimmunoprecipitation analyses with the antibody.
Shoji
S
S
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
Tashiro
A
A
Furuishi
K
K
Takenaka
O
O
Kida
Y
Y
Horiuchi
S
S
Funakoshi
T
T
Kubota
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000032:analysis / Q000276:immunology; D001358:Avian Sarcoma Viruses / Q000378:metabolism; D002642:Chick Embryo; D015683:Gene Products, gag; D005998:Glycine / Q000031:analogs & derivatives / Q000276:immunology; D006678:HIV / Q000378:metabolism; D015488:HIV Antigens / Q000032:analysis; D015368:Human T-lymphotropic virus 1 / Q000378:metabolism; D015151:Immunoblotting; D007163:Immunosorbent Techniques; D008969:Molecular Sequence Data; D019814:Myristic Acid; D009227:Myristic Acids / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D015688:Oncogene Protein pp60(v-src); D011863:Radioimmunoassay; D012190:Retroviridae / Q000378:metabolism*; D012191:Retroviridae Proteins / Q000032:analysis*; D015682:Retroviridae Proteins, Oncogenic; D014764:Viral Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus
D000906:Antibodies; D000956:Antigens, Viral; D015683:Gene Products, gag; D015488:HIV Antigens; D009227:Myristic Acids; D012191:Retroviridae Proteins; D015682:Retroviridae Proteins, Oncogenic; D014764:Viral Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus; C054858:p17 protein, Human Immunodeficiency Virus Type 1; C060955:p19 protein, Human T-lymphotropic virus 1; D019814:Myristic Acid; C057393:N-myristoylglycine; D015688:Oncogene Protein pp60(v-src); D005998:Glycine