Rapid isolation of moderate and highly polymorphic DNA fragments mapping close to WT (Wilms' tumour) and AN2 (aniridia) on chromosome 11.
Genes implicated in the development of Wilms' tumour (WT) and aniridia (AN2) have been localised to a sub-region of band p13 on chromosome 11 by molecular and cytogenetic characterisation of WAGR syndrome patients carrying variable constitutional deletions. Polymorphic markers for the region would be valuable for linkage analysis in the familial forms of both Wilms' tumour and aniridia, as well as for studying somatic rearrangements of chromosome 11 in a variety of tumour types. Here we describe the isolation and characterisation of three frequently polymorphic arbitrary DNA fragments that map proximal to the AN2 and WT loci.
Boyd
P A
PA
MRC Clinical and Population Cytogenetics Unit, Western General Hospital, Edinburgh, UK.
Christie
S
S
Hastie
N D
ND
Porteous
D J
DJ
D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D005819:Genetic Markers; D006801:Humans; D007498:Iris / Q000002:abnormalities*; D007680:Kidney Neoplasms / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D009396:Wilms Tumor / Q000235:genetics*
D005819:Genetic Markers; D004247:DNA