title: Accurate prenatal diagnosis with novel polymerase chain reaction primers in a family with sporadic hemophilia A.
abstract: Novel oligonucleotide primers are described for the convenient and internally controlled detection of the BclI factor VIII polymorphism on agarose gels. The primers were used for prenatal diagnosis in a family in which only one individual was affected with hemophilia A. In such families, it is important for the mother to understand that uncertainty about the point of origin of the mutation precludes the accurate diagnosis of affected fetuses but does allow the accurate diagnosis of unaffected fetuses in many cases.
authors.affiliation: Department of Biochemistry and Molecular Biology, Mayo Clinic/Foundation, Rochester, Minnesota.
authors.forename: G
authors.initials: G
authors.lastname: Sarkar
authors.forename: M I
authors.initials: MI
authors.lastname: Evans
authors.forename: S
authors.initials: S
authors.lastname: Kogan
authors.forename: J
authors.initials: J
authors.lastname: Lusher
authors.forename: S S
authors.initials: SS
authors.lastname: Sommer
mesh_terms: D015139:Blotting, Southern; D004259:DNA-Directed DNA Polymerase; D004587:Electrophoresis, Agar Gel; D005260:Female; D005784:Gene Amplification; D005817:Genetic Counseling; D006467:Hemophilia A / Q000175:diagnosis* / Q000235:genetics; D006801:Humans; D008297:Male; D010375:Pedigree; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011296:Prenatal Diagnosis; D019914:Taq Polymerase
chemical_list: D019914:Taq Polymerase; D004259:DNA-Directed DNA Polymerase