title: Salt stimulation of the activation of latent protein phosphatase, Fc.M, by Mn++ and by Mn/trypsin.
abstract: The activation of porcine heart latent protein phosphatase (Fc.M) by pretreatment with Mn++ followed by trypsin (Mn/trypsin) can be stimulated 2.5-fold by including NaCl or KCl in the activation mixtures. The salts also stimulated the activation of the enzyme by Mn++ to the same level as that obtained by Mn/trypsin pretreatment in the absence of salt. The presence of salt in both the Mn++ and Mn/trypsin activations decreased the Mn++ requirement 10-fold in each case. Treatment of latent Fc.M by Mn/trypsin in the presence of 0.2 M NaCl or KCl offers a convenient method of expressing the full potential activity of the protein phosphatase.
authors.affiliation: Department of Biochemistry, North Dakota State University, Fargo 58105.
authors.forename: M D
authors.initials: MD
authors.lastname: Schuchard
authors.forename: S D
authors.initials: SD
authors.lastname: Killilea
mesh_terms: D000818:Animals; D004357:Drug Synergism; D004789:Enzyme Activation / Q000187:drug effects; D008345:Manganese / Q000494:pharmacology*; D009206:Myocardium / Q000201:enzymology*; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D011189:Potassium Chloride / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013552:Swine; D014357:Trypsin / Q000378:metabolism*
chemical_list: D008345:Manganese; D012965:Sodium Chloride; D011189:Potassium Chloride; D010749:Phosphoprotein Phosphatases; D014357:Trypsin