Induction of hemopoietic chimerism in the caprine fetus by intraperitoneal injection of fetal liver cells.
Intraperitoneal injection of allogeneic liver cells from 43-day-old male fetuses into normal 60-day female goat fetuses resulted in persistent hemopoietic chimerism in surviving recipients without clinical evidence of graft-versus-host disease. Transplantation of normal fetal liver cells into preimmunocompetent goat fetuses affected with beta-D-mannosidosis may provide an alternative strategy for evaluating hemopoietic stem cell transplantation in the treatment of human lysosomal storage diseases.
Pearce
R D
RD
Department of Pediatrics, University of Toronto, Ontario, Canada.
Kiehm
D
D
Armstrong
D T
DT
Little
P B
PB
Callahan
J W
JW
Klunder
L R
LR
Clarke
J T
JT
D000818:Animals; D016026:Bone Marrow Transplantation; D002678:Chimera; D004195:Disease Models, Animal; D005260:Female; D005333:Fetus / Q000276:immunology*; D006041:Goats; D018380:Hematopoietic Stem Cell Transplantation; D007108:Immune Tolerance; D008099:Liver / Q000196:embryology*; D016031:Liver Transplantation; D008297:Male; D008363:alpha-Mannosidosis / Q000628:therapy