The effects of anxiolytic and anxiogenic benzodiazepine receptor ligands on motor activity and levels of ascorbic acid in the nucleus accumbens and striatum of the rat.
The effects of the anxiolytic benzodiazepine flurazepam and the anxiogenic beta-carboline N-methyl-beta-carboline-3-carboxylate (FG 7142) were measured in unanaesthetised rats. Changes in motor activity, using a Doppler-shift microwave device, and in the extracellular concentration of ascorbate in the striatum and nucleus accumbens, using linear sweep voltammetry with carbon paste electrodes, were monitored continuously over a period of 7 days. Both motor activity and release of ascorbate were greater during the dark than the light period; regression analysis showed a high correlation coefficient for motor activity vs release of ascorbate. The two drugs caused similar changes in this diurnal pattern. A single intraperitoneal injection of either flurazepam or FG 7142 at the end of the light period was followed by a reduction in the nocturnal rise of motor activity and of levels of ascorbate in both the nucleus accumbens and striatum. However, whereas the correlation coefficient for motor activity vs the level of ascorbate in both the nucleus accumbens and striatum remained high after the injection of flurazepam, there was a breakdown of the correlation on the day after the injection of FG 7142, followed by recovery.
Brose
N
N
University Laboratory of Physiology, Oxford, U.K.
O'Neill
R D
RD
Boutelle
M G
MG
Fillenz
M
M
D000818:Animals; D014151:Anti-Anxiety Agents / Q000494:pharmacology*; D001007:Anxiety / Q000139:chemically induced*; D001067:Appetite Depressants / Q000494:pharmacology; D001205:Ascorbic Acid / Q000378:metabolism*; D002243:Carbolines / Q000494:pharmacology; D002940:Circadian Rhythm; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism; D004563:Electrochemistry; D005479:Flurazepam / Q000494:pharmacology; D008024:Ligands; D008297:Male; D009043:Motor Activity / Q000187:drug effects*; D009714:Nucleus Accumbens / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011963:Receptors, GABA-A / Q000187:drug effects*
D014151:Anti-Anxiety Agents; D001067:Appetite Depressants; D002243:Carbolines; D008024:Ligands; D011963:Receptors, GABA-A; C035874:FG 7142; D005479:Flurazepam; D001205:Ascorbic Acid