title: Effect of freezing and ultrasonication on the density of human lymphocyte beta 2-adrenoceptors.
abstract: In this study 21 pregnant volunteers were divided into three equal groups; blood samples were taken from each, and after isolation of the lymphocytes the cell suspensions were divided into two equal samples. The density of beta 2-adrenoceptor in intact lymphocytes was compared with the receptor density for lymphocytes stored 7 days or 19 days at -70 degrees C and with cells disrupted by ultrasonication. Storage by lymphocytes at -70 degrees C for 7 days to 19 days decreased the beta 2-adrenoceptor density by 20% and 37%, respectively; ultrasonication decreased the density by 65%.
authors.affiliation: Department of Obstetrics and Gynaecology, Kuopio University Central Hospital, Finland.
authors.forename: M
authors.initials: M
authors.lastname: Santala
authors.forename: O
authors.initials: O
authors.lastname: Castrén
authors.forename: S
authors.initials: S
authors.lastname: Saarikoski
authors.forename: I
authors.initials: I
authors.lastname: Penttilä
mesh_terms: D000328:Adult; D002469:Cell Separation; D005260:Female; D005615:Freezing; D006801:Humans; D007958:Leukocyte Count; D008214:Lymphocytes / Q000378:metabolism*; D011247:Pregnancy; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D014465:Ultrasonics
chemical_list: D011943:Receptors, Adrenergic, beta