title: [Na,K-ATPase activity in the myocardial sarcolemma in ischemia].
abstract: The total time-controlled ischemia (up to 45 min) was studied for its effect on the Na,K-ATPase activity, content of nonesterified fatty acids (NEFA) and intensity of lipid peroxidation (LP) in sarcolemmal (SL) preparations and soluble fractions (SF) from the rat and guinea-pig left ventricles. A strong correlation between Na, K-ATPase inhibition and NEFA accumulation was revealed in the SF. On the contrary, changes in the NEFA content and LP level both in SL and SF did not correlate with a decrease in the enzymic activity. Pretreatment with albumin (0.5 mg/ml) induced equally small increase both in the control and in the ischemic SL preparations. It is suggested that the Na,K-ATPase activity during a short period of myocardial ischemia (up to 45 min) may be due to the NEFA accumulation in the cytosolic and/or extracellular space, but not in SL.
authors.forename: E A
authors.initials: EA
authors.lastname: Kim
authors.forename: M P
authors.initials: MP
authors.lastname: Danilenko
authors.forename: M K
authors.initials: MK
authors.lastname: Murzakhmetova
authors.forename: V I
authors.initials: VI
authors.lastname: Vashchenko
authors.forename: O V
authors.initials: OV
authors.lastname: Esyrev
mesh_terms: D000818:Animals; D003327:Coronary Disease / Q000201:enzymology* / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000378:metabolism*; D006168:Guinea Pigs; D015227:Lipid Peroxidation; D008297:Male; D009206:Myocardium / Q000201:enzymology* / Q000378:metabolism; D051381:Rats; D012508:Sarcolemma / Q000201:enzymology* / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D005230:Fatty Acids, Nonesterified; D000254:Sodium-Potassium-Exchanging ATPase
vernacular_title: Na,K-ATP-aznaia aktivnost' v sarkolemme miokarda pri ishemii.