Herpes simplex virus type 1 immediate-early protein Vmw110 reactivates latent herpes simplex virus type 2 in an in vitro latency system.
Reactivation of latent herpes simplex virus type 2 (HSV-2) by the immediate-early protein Vmw110 was studied by using an in vitro latency system. Adenovirus recombinants that express Vmw110 reactivated latent HSV-2. An HSV-1 mutant possessing a deletion in a carboxy-terminal region of Vmw110 reactivated latent HSV-2, whereas mutant FXE, which has a deletion in the second exon, did not. Therefore, Vmw110 alone is required to reactivate latent HSV-2 in vitro, and the region of Vmw110 defined by the deletion in FXE is important for this process.
Harris
R A
RA
Medical Research Council Virology Unit, Institute of Virology, Glasgow, Scotland.
Everett
R D
RD
Zhu
X X
XX
Silverstein
S
S
Preston
C M
CM
D000256:Adenoviridae / Q000235:genetics; D001483:Base Sequence; D015139:Blotting, Southern; D002478:Cells, Cultured; D004279:DNA, Viral / Q000235:genetics; D006801:Humans; D017874:Immediate-Early Proteins; D009154:Mutation; D009693:Nucleic Acid Hybridization; D011994:Recombinant Proteins / Q000235:genetics / Q000502:physiology; D015183:Restriction Mapping; D018139:Simplexvirus / Q000502:physiology*; D044767:Ubiquitin-Protein Ligases; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology*
D004279:DNA, Viral; C050678:IE1 protein, Human herpesvirus 1; D017874:Immediate-Early Proteins; D011994:Recombinant Proteins; D014764:Viral Proteins; D044767:Ubiquitin-Protein Ligases; C051804:Vmw110 protein, Human herpesvirus 1