Immunohistochemical evidence for the production of tumor necrosis factor by murine MH134 tumor cells as well as monocytes in tumor lesions after systemic administration of a polyalcoholized mannoglucan from Microellobosporia grisea.
The endogenous production of tumor necrosis factor (TNF) by tumor tissues was examined. MH134 tumor cells as well as monocytes in MH134 tumor tissues produced TNF after systemic administration of a polyalcoholized mannoglucan (MGA) from Microellobrosporia grisea as shown by the indirect immunofluorescence technique. MH134 tumor cells also produced TNF when stimulated with lipopolysaccharide in vitro. These results suggest that regression of MH134 hepatoma can be ascribed to TNF produced not only by monocytes but also by tumor cells themselves in the tumor tissue.
Okutomi
T
T
Biotechnology Research Center, Teikyo University, Kanagawa, Japan.
Dobashi
K
K
Takano
E
E
Nishizawa
T
T
Morikawa
A
A
Oshima
H
H
Soma
G
G
Mizuno
D
D
Seto
T
T
Hirata
S
S
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000473:pathology; D004306:Dose-Response Relationship, Immunologic; D005455:Fluorescent Antibody Technique; D005936:Glucans / Q000008:administration & dosage* / Q000009:adverse effects; D007275:Injections, Intravenous; D008113:Liver Neoplasms; D008297:Male; D051379:Mice; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D009368:Neoplasm Transplantation; D011135:Polysaccharides, Bacterial / Q000008:administration & dosage* / Q000009:adverse effects; D011817:Rabbits; D012074:Remission Induction; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D005936:Glucans; C063114:Microellobosporia griesea polyalcoholized mannoglucan; D011135:Polysaccharides, Bacterial; D014409:Tumor Necrosis Factor-alpha