A genetic and molecular analysis of P-induced mutations at the glucose-6-phosphate dehydrogenase locus in Drosophila melanogaster.
We examined P factor induced mutations of the Zw gene of Drosophila melanogaster in order to learn more about the site specificity of such mutations. Approximately 70,000 chromosomes were screened using a powerful positive selection scheme. As only two mutants were discovered, Zw is a "cold spot" for transposable element insertion. One mutation involved a complex P element associated chromosomal rearrangement which was used to define the orientation of the gene with respect to the centromere of the X chromosome. The second mutation was either a simple, non-dysgenically induced point mutation or a very unstable insertion.
Nero
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D
Department of Genetics and Developmental, Cornell University, Ithaca, NY 14853.
Bowditch
N
N
Pickert
S
S
MacIntyre
R J
RJ
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics*; D015698:Genomic Library; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics*; D009154:Mutation; D015183:Restriction Mapping; D014960:X Chromosome / Q000648:ultrastructure
D004251:DNA Transposable Elements; D005954:Glucosephosphate Dehydrogenase