title: Spinally projecting noradrenergic neurons of the dorsolateral pontine tegmentum: a combined immunocytochemical and retrograde labeling study.
abstract: Two to three days following injection of horseradish peroxidase (HRP) into the spinal cords of 5 cats, the animals were sacrificed and perfused, and the brainstems removed and sectioned. The sections were then processed for HRP and, immunocytochemically, for tyrosine hydroxylase (TH). The dorsolateral pontine tegmentum was divided into the locus coeruleus, subcoeruleus and Kölliker-Fuse nucleus; the mean percentage of pontospinal neurons containing TH were found to be 85.5 +/- 2.5 (S.E.M.), 79.6 +/- 5.6 and 87.1 +/- 3.1, respectively. The cell diameters of locus coeruleus cells were also measured.
authors.affiliation: Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology College of Veterinary Medicine, Washington State University, Pullman 99164-6520
authors.forename: V K
authors.initials: VK
authors.lastname: Reddy
authors.forename: S J
authors.initials: SJ
authors.lastname: Fung
authors.forename: H
authors.initials: H
authors.lastname: Zhuo
authors.forename: C D
authors.initials: CD
authors.lastname: Barnes
mesh_terms: D000320:Adrenergic Fibers / Q000166:cytology* / Q000201:enzymology; D000818:Animals; D002415:Cats; D004525:Efferent Pathways / Q000033:anatomy & histology; D006735:Horseradish Peroxidase; D007150:Immunohistochemistry; D008125:Locus Coeruleus / Q000166:cytology / Q000201:enzymology; D011149:Pons / Q000166:cytology* / Q000201:enzymology; D013116:Spinal Cord / Q000166:cytology*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D006735:Horseradish Peroxidase; D014446:Tyrosine 3-Monooxygenase