title: Mannose 6-phosphate receptor in porcine thyroid follicle cells. Localization and possible implications for the intracellular transport of thyroglobulin.
abstract: Thyroglobulin has been shown to be phosphorylated and to carry the mannose 6-phosphate (M6P) signal in terminal position. In order to investigate whether the cation-independent mannose 6-phosphate receptor (CI-MPR) can possibly play a role in the transport of thyroglobulin the localization of the receptor was analyzed in thyroid follicle cells. The immunocytochemical observations showed that the CI-MPR is primarily located in elements of the endocytic pathway such as coated pits and endosomes. This localization of the CI-MPR in thyrocytes differs from the receptor sites in other cell types by the rare occurrence of the CI-MPR in cisternae of the Golgi complex. The observations are interpreted as an indication that the relatively small amount of receptor in the Golgi complex might be occupied primarily by lysosomal hydrolases. The CI-MPR in thyrocytes might, therefore, be unable to bind and to convey thyroglobulin efficiently. The receptor is, however, a binding site for thyroglobulin at the apical plasma membrane and may, therefore, be involved in the binding of thyroglobulin and its transfer from the follicle lumen to lysosomes.
authors.affiliation: Institute for Cell Biology, University of Munich/Federal Republic of Germany.
authors.forename: G
authors.initials: G
authors.lastname: Scheel
authors.forename: V
authors.initials: V
authors.lastname: Herzog
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D004705:Endocytosis; D007150:Immunohistochemistry; D017527:Receptor, IGF Type 2; D011956:Receptors, Cell Surface / Q000378:metabolism*; D013552:Swine; D013954:Thyroglobulin / Q000378:metabolism*; D013961:Thyroid Gland / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D000911:Antibodies, Monoclonal; D017527:Receptor, IGF Type 2; D011956:Receptors, Cell Surface; D013954:Thyroglobulin