Measurement of angiotensin II concentration in rat plasma: pathophysiological applications.
A very reliable isocratic reverse phase high performance liquid chromatography (HPLC) system has been developed to separate angiotensins, which combined with a very sensitive radioimmunoassay, provided precise measurements of the endogenous angiotensin II (AII) concentration in the rat plasma in different experimental circumstances. The overall recoveries of AII were 95.2 +/- 15.8% (means +/- SD) when 10 pg of this peptide was added to 1 ml of human plasma. The coefficient of variation for within-assay precision was 10% (n = 6). The plasma AII, measured by this method, of normal male pentobarbital-anesthetized rats was 53.0-141.6 pg/ml (mean: 103.9 +/- 29.7 pg/ml). The plasma AII of rats fed a sodium deficient diet was 300.0 +/- 100.6 pg/ml, while that of rats given oral Enalapril, an angiotensin converting enzyme (ACE) inhibitor, for 1 week was 35.7 +/- 28.0 pg/ml. The plasma AII of bilaterally nephrectomized rats was 2.7 +/- 2.9 pg/ml 24 hours after nephrectomy and below the detection limit 48 hr after nephrectomy. This method, therefore, can be used to study AII in different pathophysiological states or after treatment with renin-angiotensin system inhibitors.
Huang
H
H
INSERM Unit 36-17, Paris, France.
Baussant
T
T
Reade
R
R
Michel
J B
JB
Corvol
P
P
D000704:Analysis of Variance; D000758:Anesthesia; D000804:Angiotensin II / Q000097:blood*; D000805:Angiotensin III / Q000097:blood; D000809:Angiotensins / Q000097:blood; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004656:Enalapril / Q000494:pharmacology; D008297:Male; D009994:Osmolar Concentration; D010424:Pentobarbital; D010446:Peptide Fragments / Q000097:blood; D011863:Radioimmunoassay; D051381:Rats / Q000097:blood*; D011919:Rats, Inbred Strains; D012083:Renin / Q000097:blood
D000809:Angiotensins; D010446:Peptide Fragments; D000804:Angiotensin II; D000805:Angiotensin III; C022782:angiotensin pentapeptide; D004656:Enalapril; D012083:Renin; D010424:Pentobarbital