title: Serological reactions in sheep and cattle experimentally infected with three Australian isolates of bluetongue virus.
abstract: Serums from 103 sheep and 24 cattle experimentally infected with one of 3 serotypes of bluetongue virus isolated in Australia were tested for antibody to bluetongue virus in the serum neutralisation test and the agar gel diffusion precipitin test. Antibody to bluetongue virus was first detected by these tests 8 to 10 days after intravenous infection in 4 sheep that were bled daily for serum analysis. The agar gel diffusion test failed to detect antibody in 28% (29/103) of sheep which had seroconverted in the serum neutralisation test. A further 7% (7/103) of sheep serums were negative in both tests 14 to 22 d after infection. Both tests detected antibody to bluetongue virus in all cattle serums by 10 days after detection of viraemia. In comparison with the intravenous route of infection, extended prepatent periods for the commencement of viraemia resulting from intradermal, subcutaneous and intrauterine routes of infection in the cattle caused corresponding delays in the detection of antibody. For example, one cow that was infected by intrauterine inoculation did not become viraemic until 22 d after inoculation and antibody was not detected until 32 d after inoculation.
authors.affiliation: CSIRO, Division of Tropical Animal Science, Long Pocket Laboratories, Indooroopilly, Queensland.
authors.forename: K R
authors.initials: KR
authors.lastname: Squire
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D001315:Australia; D001819:Bluetongue / Q000276:immunology*; D001820:Bluetongue virus / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology*; D005260:Female; D005779:Immunodiffusion / Q000662:veterinary; D008297:Male; D009500:Neutralization Tests / Q000662:veterinary; D011237:Predictive Value of Tests; D012087:Reoviridae / Q000276:immunology*; D012756:Sheep; D014766:Viremia / Q000662:veterinary
chemical_list: D000914:Antibodies, Viral