Antimicrobial activity of synthetic bactenecin.
Bactenecin is an antimicrobial peptide isolated from bovine neutrophils. Bactenecin was synthesized by solid-phase peptide synthesis and renatured to a fully disulfide bonded form. The peptide inhibits the growth of Escherichia coli and Staphylococcus aureus at the same concentration reported for the peptide purified from bovine neutrophils. Bactenecin inhibits the growth of other medically important bacteria and yeast, and it kills the fungus Trichophyton rubrum. Acetylation and amidation of the amino- and carboxy-termini of bactenecin do not change its potency, while replacement of its two cysteine residues with serine decreases the potency.
Gallis
B
B
Immunex Corporation, Seattle, Washington 98101.
Mehl
J
J
Prickett
K S
KS
Martin
J A
JA
Merriam
J
J
March
C J
CJ
Cerretti
D P
DP
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides; D001419:Bacteria / Q000187:drug effects; D002478:Cells, Cultured; D005658:Fungi / Q000187:drug effects; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D054806:Magainins; D008969:Molecular Sequence Data; D010455:Peptides / Q000494:pharmacology; D010456:Peptides, Cyclic / Q000138:chemical synthesis / Q000494:pharmacology*; D029867:Xenopus Proteins
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D054806:Magainins; D010455:Peptides; D010456:Peptides, Cyclic; D029867:Xenopus Proteins; C053301:magainin 2 peptide, Xenopus; C056454:bactenecin