Neuromuscular recovery using calcium protease inhibition after median nerve repair in primates.
Inhibition of calcium-activated neutral protease, in muscle and nerve, by the tripeptide leupeptin after median nerve transection and epineural repair in monkeys (Cebus apella) was studied. Results indicate that inhibition of the protease after nerve repair facilitates morphologic recovery in denervated thenar muscles and in distal thenar nerve branches. In addition, functional recovery was facilitated in leupeptin-treated animals after nerve repair as measured by sensory and motor conduction velocities. Toxicologic testing showed that leupeptin, administered at 18 mg/kg, intramuscularly, twice daily, for 6 months did not adversely affect hematology, clotting, or plasma complement component C3 profiles. These data indicate that leupeptin is an effective and safe adjunct to peripheral nerve repair.
Badalamente
M A
MA
Department of Orthopaedics, School of Medicine, State University of New York, Stony Brook 11794-8181.
Hurst
L C
LC
Stracher
A
A
D000818:Animals; D002154:Calpain / Q000037:antagonists & inhibitors*; D002428:Cebus; D005455:Fluorescent Antibody Technique; D007976:Leupeptins / Q000494:pharmacology*; D008475:Median Nerve / Q000187:drug effects / Q000502:physiology*; D009046:Motor Neurons / Q000187:drug effects / Q000502:physiology; D009416:Nerve Regeneration / Q000187:drug effects*; D009431:Neural Conduction / Q000187:drug effects; D009469:Neuromuscular Junction / Q000187:drug effects / Q000502:physiology*; D009475:Neurons, Afferent / Q000187:drug effects / Q000502:physiology; D009842:Oligopeptides / Q000494:pharmacology*; D013997:Time Factors
D007976:Leupeptins; D009842:Oligopeptides; D002154:Calpain