title: Effect of cytochrome c concentration on the ultrastructural appearance of bovine nasal cartilage proteoglycans.
abstract: Bovine nasal cartilage proteoglycan monomers were studied by Kleinschmidt and Zahn's molecular spreading technique as modified by Rosenberg et al. By decreasing the cytochrome c concentration in the epiphase to 2 micrograms per 100 microliters we were able on nitrocellulose-coated grids routinely to obtain highly contrasted and well spread proteoglycan monomers with a characteristic brush-like appearance and, sometimes, a clearly distinguishable hyaluronic acid binding region. Previously, a hyaluronic acid binding region has only been observed routinely in spread proteoglycan aggregates, and a brush-like structure of proteoglycan monomers on carbon-coated grids, but with considerably less precision due to the poor contrast of the molecules. Molecular spreading was further improved by decreasing the cytochrome c concentration in the epiphase to less than 2 micrograms per 100 microliters, but contrast was reduced making visualization of molecular details difficult.
authors.affiliation: Cartilage Research Laboratory, U.18 INSERM, Lariboisiere Hospital, Paris, France.
authors.forename: P
authors.initials: P
authors.lastname: Front
authors.forename: C
authors.initials: C
authors.lastname: Dauguet
authors.forename: D R
authors.initials: DR
authors.lastname: Mitrovic
mesh_terms: D000818:Animals; D002356:Cartilage / Q000378:metabolism* / Q000648:ultrastructure; D002417:Cattle; D003574:Cytochrome c Group; D006025:Glycosaminoglycans / Q000378:metabolism; D006652:Histological Techniques; D008854:Microscopy, Electron; D009297:Nasal Mucosa / Q000378:metabolism; D009666:Nose / Q000648:ultrastructure; D011509:Proteoglycans / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D003574:Cytochrome c Group; D006025:Glycosaminoglycans; D011509:Proteoglycans