Localization of bombesin-, neuropeptide Y-, enkephalin- and tyrosine hydroxylase-like immunoreactivities in rat coeliac-superior mesenteric ganglion.
The localization of bombesin- (BOMB) and enkephalin- (ENK) immunoreactive (IR) nerves was studied in rat coeliac-superior mesenteric ganglion complex in relation to neuropeptide Y (NPY)- and tyrosine hydroxylase (TH)-immunoreactive neurons with an immunofluorescence double-staining method. Very dense networks of BOMB-IR nerve terminals surrounded the majority of the principal ganglion cells, whether or not they were TH-IR. BOMB-IR nerves were specifically related to the non-NPY-IR neurons. Moderately dense networks of ENK-IR fibers were unevenly distributed among the ganglion cells. Majority of these neurons exhibited TH-IR and some of them also contained NPY-IR. In sections double stained with antibodies to ENK and BOMB some nerve fibers contained both peptides. The findings suggest that BOMB-IR nerves, which have been previously demonstrated to originate from gut, control the function of non-NPY-IR ganglion cells. ENK-IR nerves apparently control the adrenergic neurons which project to gut and also some NPY-IR vasomotoric neurons. The finding that ENK- and BOMB-IR coexist in some nerves suggests that some ENK-IR nerves may originate from gut, although the major part probably represents preganglionic fibers originating from spinal cord.
Järvi
R
R
University of Tampere, Department of Biomedical Sciences, Finland.
D000818:Animals; D001839:Bombesin / Q000032:analysis*; D004745:Enkephalins / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D005728:Ganglia, Sympathetic / Q000032:analysis* / Q000201:enzymology; D008297:Male; D009474:Neurons / Q000032:analysis; D009478:Neuropeptide Y / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis*
D004745:Enkephalins; D009478:Neuropeptide Y; D014446:Tyrosine 3-Monooxygenase; D001839:Bombesin