Multiple high-frequency transpositions of Tn5 in Serratia marcescens 274 from a thermosensitive replication mutant of plasmid R388.
We have used the broad-host-range conjugative suicide plasmid vector described by Sasakawa and Yoshikawa [Gene 56 (1987) 283-288] for transposon mutagenesis of Serratia marcescens 274. We report multiple transposition events of Tn5 from this vector. In addition, the unusual pattern of Tn5 transposition might provide an insight into its regulation.
Estepa
G R
GR
Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037.
Harshey
R M
RM
D015139:Blotting, Southern; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D006358:Hot Temperature; D009154:Mutation; D010957:Plasmids / Q000235:genetics*; D012706:Serratia marcescens / Q000235:genetics*
D004251:DNA Transposable Elements