[Comparative test of the soluble toxicity of hydroxyapatite ceramics using human and animal osteoblasts].
The soluble toxicity of 5 different hydroxylapatite granulates was tested in osteoblast cell cultures. We established a human osteoblast-like cell culture and a culture of rat (Lewis) osteoblast-like cells. The granulates were also tested in cultures of human gingival fibroblasts as a conventional system. It is demonstrated that the osteoblast-like cell cultures are of higher sensitivity than fibroblast cultures which do not show any reaction to the extracts of hydroxylapatite granulates. Only 2 hydroxylapatite granulates show some toxic. The human osteoblast-like cells reacted slightly more sensitive to toxic substances of the materials tested than rat osteoblast-like cells. It is demonstrated that the osteoblast-like cell cultures are a highly sensitive test system for materials with low toxicity.
Lang
H
H
Kruppenbacher
J P
JP
Mertens
T
T
D000818:Animals; D001672:Biocompatible Materials; D002478:Cells, Cultured; D017886:Durapatite; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D006882:Hydroxyapatites / Q000633:toxicity*; D010006:Osteoblasts / Q000187:drug effects*
D001672:Biocompatible Materials; D006882:Hydroxyapatites; D017886:Durapatite
Toxizität von Hydroxylapatitkeramiken auf menschliche und tierische Osteoblasten.