Transcriptional trans-repression by the c-myb proto-oncogene product.
We report that the c-myb protein binds to another site, MBS-II, in the SV40 enhancer with low affinity. In co-transfection experiments with a c-myb expression plasmid, tandem repeats of the sequence containing the MBS-II site induced c-myb-dependent transcriptional repression. Results of mutational analyses of the sequence around the MBS-II site suggested that the c-myb protein represses transcription by competing with another trans-activator. These results indicate that c-myb protein can regulate transcription not only positively but also negatively.
Nakagoshi
H
H
Laboratory of Molecular Genetics, Institute of Physical and Chemical Research (RIKEN), Ibaraki, Japan.
Nagase
T
T
Ueno
Y
Y
Ishii
S
S
D002874:Chromosome Mapping; D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins / Q000502:physiology; D003850:Deoxyribonuclease I; D004742:Enhancer Elements, Genetic; D015967:Gene Expression Regulation, Viral; D066298:In Vitro Techniques; D010957:Plasmids; D011518:Proto-Oncogene Proteins / Q000502:physiology*; D020598:Proto-Oncogene Proteins c-myb; D012045:Regulatory Sequences, Nucleic Acid; D012097:Repressor Proteins / Q000502:physiology*; D013539:Simian virus 40 / Q000235:genetics; D014157:Transcription Factors / Q000502:physiology*; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D011518:Proto-Oncogene Proteins; D020598:Proto-Oncogene Proteins c-myb; D012097:Repressor Proteins; D014157:Transcription Factors; D003850:Deoxyribonuclease I