title: Blastocoel expansion in the preimplantation mouse embryo: stimulation of sodium uptake by cAMP and possible involvement of cAMP-dependent protein kinase.
abstract: Elevating cAMP levels in mouse blastocysts increases the rate of blastocoel expansion (F. Manejwala, E. Kaji, and R. M. Schultz, 1986, Cell, 46, 95-103), which requires extracellular sodium (F. Manejwala, E. J. Crago, Jr., and R. M. Schultz, 1989, Dev. Biol. 133, 210-220). We report that cAMP analogs that can activate the cAMP-dependent protein kinase stimulate 22Na+ uptake by cavitating mouse blastocysts and that inhibitors of cAMP-dependent protein kinase activity inhibit the cAMP-stimulated increase in both the rate of blastocoel expansion and 22Na+ uptake.
authors.affiliation: Department of Biology, University of Pennsylvania, Philadelphia 19104-6018.
authors.forename: F M
authors.initials: FM
authors.lastname: Manejwala
authors.forename: R M
authors.initials: RM
authors.lastname: Schultz
mesh_terms: D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D001755:Blastocyst / Q000502:physiology*; D000242:Cyclic AMP / Q000502:physiology*; D047108:Embryonic Development; D005110:Extracellular Space / Q000502:physiology; D005260:Female; D066298:In Vitro Techniques; D007546:Isoquinolines / Q000494:pharmacology; D051379:Mice; D010879:Piperazines / Q000494:pharmacology; D011247:Pregnancy; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000502:physiology*; D012964:Sodium / Q000378:metabolism*
chemical_list: D007546:Isoquinolines; D010879:Piperazines; D047428:Protein Kinase Inhibitors; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; C053177:N-(2-(methylamino)ethyl)-5-isoquinolinesulfonamide; D012964:Sodium; D000242:Cyclic AMP; D011494:Protein Kinases