Properties of a cyclic 3'5'-nucleotide phosphodiesterase from Vigna mungo.
Cyclic AMP phosphodiesterase (PDE) partially purified from roots of Vigna mungo exhibited optimum activity at pH 5.5 to 6.0 and maximum enzyme activity at 50 degrees C. Levels of PDE activity in roots remained relatively constant from the first to the eleventh day after germination; on the twelfth day there was a 400% increase in PDE activity. The enzyme was stable for at least 48 hours at 28 degrees C, retaining 92% of its original activity. Plant growth hormones including gibberellic acid, indoleacetic acid and kinetin at 1.0 and 10.0 microM concentrations did not have any significant effect on enzyme activity. Nucleotides tested including cyclic 2'3' AMP, cyclic 2'3' GMP completely abolished enzyme activity at 1.0mM while cyclic 3'5' GMP, cyclic 3'5' GMP, 2'deoxy 5' ATP, 2'deoxy 5'GTP and 5'ADP were also inhibitory to the enzyme. The enzyme was stimulated by Mg2+, Fe2+ and NH4+ while Cu2+ and Fe3+ were inhibitory. Theophylline, caffeine, phosphate, pyrophosphate and EDTA were inhibitory to the enzyme.
Lee
C H
CH
Dept. of Biochemistry and Microbiology, Univ. of Agriculture Malaysia, Selangor.
Abidin
U Z
UZ
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism*; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000494:pharmacology; D009712:Nucleotides, Cyclic / Q000494:pharmacology; D010710:Phosphates / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology; D010944:Plants / Q000187:drug effects / Q000201:enzymology*
D008670:Metals; D009712:Nucleotides, Cyclic; D010710:Phosphates; D010937:Plant Growth Regulators; D015105:3',5'-Cyclic-AMP Phosphodiesterases