title: Reconstitution of a light-stimulated adenylate cyclase from retina and Neurospora crassa preparations. Characterization of the heterologous systems using normal and degenerative retinas.
abstract: Adenylate cyclase catalytic subunits from Neurospora crassa membranes may interact with regulatory factors from membranes of bovine retinal rod outer segments (pretreated with N-ethylmaleimide), reconstituting a heterologous system which, in the presence of light, is catalytically active in assay mixtures containing MgATP. Maximal activation was observed at 550 nm. Transducin-depleted retinal membranes were not capable of reconstituting the heterologous light-stimulated adenylate cyclase system. Addition of a transducin preparation to depleted membranes restored the reconstitution capacity of these membranes. A similar heterologous adenylate cyclase system was reconstituted with Neurospora and mouse retinal whole membranes (pretreated with N-ethylmaleimide). Membranes from mice suffering photoreceptor degeneration (rd homozygotes) did not reconstitute an heterologous adenylate cyclase system.
authors.affiliation: Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Buenos Aires, Argentina.
authors.forename: J P
authors.initials: JP
authors.lastname: Muschietti
authors.forename: G M
authors.initials: GM
authors.lastname: Bianchini
authors.forename: H E
authors.initials: HE
authors.lastname: Martinetto
authors.forename: V C
authors.initials: VC
authors.lastname: Carricarte
authors.forename: N
authors.initials: N
authors.lastname: Giusto
authors.forename: D B
authors.initials: DB
authors.lastname: Farber
authors.forename: H N
authors.initials: HN
authors.lastname: Torres
authors.forename: M M
authors.initials: MM
authors.lastname: Flawia
mesh_terms: D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000262:Adenylyl Cyclases / Q000032:analysis*; D000818:Animals; D002417:Cattle; D006152:Cyclic GMP / Q000378:metabolism; D008027:Light; D051379:Mice; D008810:Mice, Inbred C57BL; D009491:Neurospora / Q000201:enzymology*; D009492:Neurospora crassa / Q000201:enzymology*; D012160:Retina / Q000201:enzymology*
chemical_list: D000246:Adenosine Diphosphate Ribose; D000262:Adenylyl Cyclases; D006152:Cyclic GMP