[Regional, cellular, subcellular distribution of neurospecific protein-hormonal complexes].
A sensitive radioimmunological assay (RIA) has been developed to detect the tissue specificity and subcellular localization of three specific protein-hormonal complexes (PHC) of the hypothalamus which have a regulatory function in the brain and visceral organs. Using the highly specific rabbit antisera to beef PHC an order of increasing immunoreactivity in different areas of CNS is as follows: hypothalamus, cerebellum, occipital cortex. The PHC-like immunoreactivity (IR) is found in the neurosecretory granules of the hypothalamo-neurohypophyseal system (about 30%) and in the synaptosomal fraction (70%). In the myelin, mitochondrial and nuclear fractions the PHC-like IR is not revealed. IR of PHC is 1000-fold higher in the brain than in visceral organs (heart, skeletal muscles, adrenals, pancreas and blood serum). A possible role of the PHC as markers of the neuroendocrine cells is discussed.
Srapionian
R M
RM
Abelian
Zh G
ZhG
Galoian
A A
AA
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D002415:Cats; D002417:Cattle; D002478:Cells, Cultured; D007150:Immunohistochemistry; D018377:Neurotransmitter Agents / Q000378:metabolism*; D011863:Radioimmunoassay; D051381:Rats; D014781:Viscera / Q000378:metabolism*
D002352:Carrier Proteins; D018377:Neurotransmitter Agents
Regional'noe, kletochnoe, subkletochnoe raspredelenie neĭrospetsificheskikh belok-gormonal'nykh kompleksov.