title: [Production, purification and evaluation of human cytomegalovirus 66 kD structural antigen for antibody detection by ELISA].
abstract: An ELISA method using human CMV major polypeptide (66 kD) purified by electroelution was used to detect IgG and IgM antibodies and was compared with a conventional ELISA using the whole virus. The ELISA-66 kD showed a good sensitivity (98.2%) and had a better specificity than the ELISA-whole virus (97.5% versus 80.3%). Cross reactions observed, particularly with sera containing anti-EBV antibodies, were ruled out.
authors.affiliation: Service de Bactériologie Virologie B, Centre Hospitalier Regional, IRFPPS, Lille, France.
authors.forename: P
authors.initials: P
authors.lastname: Gireaudot
authors.forename: A
authors.initials: A
authors.lastname: Dewilde
authors.forename: P E
authors.initials: PE
authors.lastname: Lobert
authors.forename: L
authors.initials: L
authors.lastname: Bocket-Mouton
authors.forename: P
authors.initials: P
authors.lastname: Wattre
mesh_terms: D000956:Antigens, Viral / Q000276:immunology / Q000302:isolation & purification*; D003587:Cytomegalovirus / Q000276:immunology* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis*
chemical_list: D000956:Antigens, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M
vernacular_title: Production, purification et évaluation de l'antigène de structure 66 kD du cytomégalovirus humain pour la détection des anticorps par ELISA.