Immunoprecipitation of labeled antigens with Eupergit C1Z.
Eupergit C1Z, a nonporous sedimentable acrylic polymer is shown to be a convenient solid support for isolation of antigens with high purity from small amounts of detergent-solubilized cells. Several applications of Eupergit C1Z coupled with antibodies and anti-antibodies are described, and compared with two other methods of immunoprecipitation.
Grässel
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Institut für Physiologische Chemie und Pathobiochemie der Universität Münster, Federal Republic of Germany.
Röling
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Hasilik
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D000180:Acrylic Resins; D000818:Animals; D000906:Antibodies; D000941:Antigens / Q000302:isolation & purification*; D002402:Cathepsin D / Q000276:immunology / Q000302:isolation & purification; D002478:Cells, Cultured; D006801:Humans; D007074:Immunoglobulin G; D009113:Muramidase / Q000276:immunology / Q000302:isolation & purification; D009195:Peroxidase / Q000276:immunology / Q000302:isolation & purification; D011233:Precipitin Tests / Q000379:methods*; D011817:Rabbits
D000180:Acrylic Resins; D000906:Antibodies; D000941:Antigens; D007074:Immunoglobulin G; C061522:Eupergit C1Z; D009195:Peroxidase; D009113:Muramidase; D002402:Cathepsin D