title: Direct induction of MHC class I, but not class II, expression on endothelial cells by cytomegalovirus infection.
abstract: MHC expression on endothelial cells after cytomegalovirus infection was investigated using double antibody radioimmunoassays and immunofluorescence flow cytometry. After CMV infection there was an increase in class I and beta 2 microglobulin expression. This increase corresponds to the percentage of cells that are infected with CMV. There was no evidence that the increased class I expression was due to soluble factors produced by the infected cells. Although CMV only affects approximately 10% of the endothelial cells, class I and beta 2 microglobulin expression on the cell population exceeds that induced by an optimal dose of alpha-interferon. Class II expression is not increased after CMV infection. However, when gamma-interferon was added to the medium after CMV infection a normal increase in class II expression was found, suggesting that CMV infection does not inhibit the gamma-interferon-induced expression of these molecules.
authors.affiliation: Department of Nephrology, University Hospital Leiden, The Netherlands.
authors.forename: W T
authors.initials: WT
authors.lastname: van Dorp
authors.forename: E
authors.initials: E
authors.lastname: Jonges
authors.forename: C A
authors.initials: CA
authors.lastname: Bruggeman
authors.forename: M R
authors.initials: MR
authors.lastname: Daha
authors.forename: L A
authors.initials: LA
authors.lastname: van Es
authors.forename: F J
authors.initials: FJ
authors.lastname: van Der Woude
mesh_terms: D002478:Cells, Cultured; D003586:Cytomegalovirus Infections / Q000276:immunology*; D004730:Endothelium, Vascular / Q000276:immunology*; D006681:HLA-D Antigens / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D006801:Humans; D007158:Immunologic Techniques; D007370:Interferon Type I / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology; D008222:Lymphokines / Q000096:biosynthesis
chemical_list: D006681:HLA-D Antigens; D015395:Histocompatibility Antigens Class I; D007370:Interferon Type I; D007376:Interleukin-2; D008222:Lymphokines; D007371:Interferon-gamma