Endothelial pump function in rabbit corneas kept under eye bank conditions.
Endothelial pump activity of rabbit corneas kept under eye bank conditions was examined. Different swelling rates for medium with and without ouabain were used for the calculation of endothelial pump rate. After an initial weight increase during 24 h, the control corneas kept approximately constant weight for 1 week. The ouabain-treated corneas increased continually, and during the time interval 24-48 h the increase was 41% of start weight. This corresponds to a pump activity of 2.3% of the weight of the normal corneal stroma per h, or to 9 microns of the corneal thickness per h. Each endothelial cell pumped fluid corresponding to 200-300% of the cell volume per h between day 1 (24 h) and day 8. The pump activity was gradually reduced after 8 days. The average weight was the same in both groups after 7 weeks.
Medin
W
W
University Eye Department, Rikshospitalet, University of Oslo, Norway.
Davanger
M
M
D000818:Animals; D003315:Cornea / Q000201:enzymology / Q000502:physiology*; D003470:Culture Media; D004727:Endothelium / Q000201:enzymology / Q000502:physiology*; D005125:Eye Banks; D010042:Ouabain / Q000494:pharmacology; D011817:Rabbits; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013997:Time Factors; D014015:Tissue Banks
D003470:Culture Media; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase