Thyroid epithelial cell transformation by a retroviral vector expressing SV40 large T.
A recombinant murine retroviral vector encoding the SV40 virus large T antigen was used to infect stably an immortal line of differentiated rat thyroid epithelial cells, FRTL-5. Expression of SV40 T transformed these cells to anchorage independence and tumorigenicity but did not alter morphology or abolish tissue-specific functions and growth factor requirements. The resulting phenotype provides a model of well-differentiated human thyroid cancer.
Burns
J S
JS
Department of Pathology, University of Wales College of Medicine, Heath Park, Cardiff, UK.
Lemoine
L
L
Lemoine
N R
NR
Williams
E D
ED
Wynford-Thomas
D
D
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004848:Epithelium / Q000473:pathology; D005822:Genetic Vectors; D010957:Plasmids; D051381:Rats; D013539:Simian virus 40 / Q000276:immunology; D013961:Thyroid Gland / Q000473:pathology*; D013964:Thyroid Neoplasms / Q000209:etiology / Q000235:genetics*
D000952:Antigens, Polyomavirus Transforming