title: Excitatory transmission in the dorsal horn is in part mediated through APV-sensitive NMDA receptors.
abstract: The role of subtypes of excitatory amino acid receptor in synaptic transmission in the spinal dorsal horn has been studied in an in vitro slice preparation with well-preserved afferent inputs to the dorsal horn. Intracellular recordings were made from 30 dorsal horn neurons in laminae I-III of 18-28 day old rats. Superfusion of the slice with a Mg2+ zero solution resulted in an increase in the amplitude and duration of dorsal root-evoked excitatory postsynaptic potentials (EPSP) recorded intracellularly from dorsal horn neurons. Bath application of D-2-amino-5-phosphonovalerate (10(-5) M to 2.5 x 10(-5) M) or DL-2-amino-5-phosphonovalerate (10(-4) M to 2.5 x 10(-4) M) rapidly and reversibly abolished the later component of the EPSP evoked by activation of either group of primary afferents and selectively antagonized the N-methyl-D-aspartate-induced depolarization.
authors.affiliation: Department of Veterinary Anatomy, Iowa State University, Ames 50011.
authors.forename: S
authors.initials: S
authors.lastname: Jeftinija
mesh_terms: D015763:2-Amino-5-phosphonovalerate; D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D004558:Electric Stimulation; D066298:In Vitro Techniques; D008274:Magnesium / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D016194:Receptors, N-Methyl-D-Aspartate; D017981:Receptors, Neurotransmitter / Q000187:drug effects / Q000502:physiology*; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D009435:Synaptic Transmission / Q000187:drug effects*; D014633:Valine / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D016194:Receptors, N-Methyl-D-Aspartate; D017981:Receptors, Neurotransmitter; D015763:2-Amino-5-phosphonovalerate; D014633:Valine; D008274:Magnesium