Comparative study of the symbiotic plasmid DNA in free living bacteria and bacteroids of Rhizobium leguminosarum.
The symbiotic plasmid (pSym) DNA present in bacteroids of strain RCR1001 of Rhizobium leguminosarum biovar viceae has been compared qualitatively and quantitatively with that present in free living bacteria by hybridization experiments with appropriate probes. A decrease in the relative amount of pSym DNA was observed in bacteroids as compared to bacteria. No rearrangements of the symbiotically expressed pSym borne genes were detected in bacteroids.
Vierny
C
C
International Institute of Genetics and Biophysics, CNR, Naples, Italy.
Iaccarino
M
M
D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000235:genetics*; D007887:Fabaceae / Q000382:microbiology; D005784:Gene Amplification; D005798:Genes, Bacterial; D009693:Nucleic Acid Hybridization; D010946:Plants, Medicinal; D010957:Plasmids; D012231:Rhizobium / Q000235:genetics* / Q000502:physiology; D013559:Symbiosis
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes