title: Detection of human papillomavirus type 16 DNA in cervical swabs and formalin-fixed, paraffin-embedded squamous cell carcinomas of non-genital tissues using a synthetic oligodeoxynucleotide probe.
abstract: The potential of using a chemically synthesized oligodeoxynucleotide as a diagnostic probe to detect human papillomavirus type 16 (HPV-16) in genital infections was evaluated by comparing it with a cloned full-length HPV-16 probe in dot-blot DNA hybridizations. An oligonucleotide sequence, 20 bases in length from the E6 region of HPV-16 (E6 oligo) and different from the DNA sequences of HPV types 6, 11 and 18 by at least 2 base pairs, was chosen for chemical synthesis. The oligoprobe, which was 5'-end labelled with [32P]dATP, was found to be specific, but approximately ten times less sensitive than the full-length radiolabelled probe of HPV-16, in dot-blot hybridizations with the DNA of HPV-6, -11, -16 and -18, HPV positive and negative cell-lines. From 36 cervical or vulval scrapes two samples were found positive with both cloned HPV-16 and oligoprobe hybridization. Of 21 samples of formalin-fixed, paraffin-embedded squamous cell carcinomas originating from anus, oesophagus, penis, colon, breast and skin only 4 anal squamous cell carcinomas were positives when hybridized with cloned HPV-16 DNA or with the oligoprobe. This study confirms that HPV-16, which is frequently associated with squamous cell carcinoma of the cervix is also strongly associated with squamous cell carcinoma of the anus.
authors.affiliation: Department of Microbiology, University of Western Australia, Nedland.
authors.forename: P
authors.initials: P
authors.lastname: Rakoczy
authors.forename: T
authors.initials: T
authors.lastname: Demeter
authors.forename: L
authors.initials: L
authors.lastname: Hutchinson
authors.forename: G F
authors.initials: GF
authors.lastname: Sterrett
authors.forename: E C
authors.initials: EC
authors.lastname: Pixley
authors.forename: J K
authors.initials: JK
authors.lastname: Kulski
mesh_terms: D001345:Autoradiography; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis*; D002460:Cell Line; D015342:DNA Probes; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D005404:Fixatives; D005557:Formaldehyde; D006801:Humans; D015345:Oligonucleotide Probes; D009928:Organ Specificity; D027383:Papillomaviridae / Q000235:genetics*; D010232:Paraffin; D014626:Vaginal Smears
chemical_list: D015342:DNA Probes; D015344:DNA Probes, HPV; D004279:DNA, Viral; D005404:Fixatives; D015345:Oligonucleotide Probes; D005557:Formaldehyde; D010232:Paraffin