[The functioning of mammalian mitochondria injected into fish embryos].
The possibility of mammalian mitochondria functioning in fish embryos has been studied. Suspension of mitochondria isolated from the mouse fibroblast B-82/cap (chloramphenicol-resistant) and B-82 (chloramphenicol sensitive) cell cultures, were injected into the fertilized loach eggs. These embryos with an artificially increased number of mouse mitochondria developed and lived till the larval stages. Activity of cytochrome oxidase in these embryos was 1.5-2 times that in the control several hours after the injection, decreased during development and reached the control level by the gastrula stage. If these embryos with artificially increased number of mouse mitochondria were incubated in presence of chloramphenicol, only embryos that contained mitochondria from chloramphenicol-resistant cells survived, thus suggesting that the injected mitochondria do not degrade but are preserved and function in the cytoplasm of developing loach embryos.
Abramova
N B
NB
Bueverova
E I
EI
Neĭfakh
A A
AA
D000818:Animals; D002458:Cell Fractionation; D002460:Cell Line; D002701:Chloramphenicol / Q000037:antagonists & inhibitors / Q000494:pharmacology; D002999:Clone Cells / Q000187:drug effects / Q000201:enzymology; D003531:Cypriniformes / Q000196:embryology* / Q000378:metabolism; D003593:Cytoplasm / Q000201:enzymology; D003576:Electron Transport Complex IV / Q000032:analysis; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology; D007267:Injections / Q000379:methods; D051379:Mice; D008928:Mitochondria / Q000201:enzymology / Q000502:physiology* / Q000637:transplantation
D002701:Chloramphenicol; D003576:Electron Transport Complex IV
Funktsionirovanie mitokhondriĭ mlekopitaiushchikh, in''etsirovannykh v zarodyshi ryb.