Guanine nucleotides stimulate hydrolysis of phosphatidyl inositol bis phosphate in human myelin membranes.
Phosphodiesterase activity was stimulated in myelin membranes in the presence of guanine nucleotide analogues. This activity was reduced in myelin membranes which had been adenosine diphosphate ribosylated in the presence of cholera toxin which ADP-ribosylated three proteins of Mr 46,000, 43,000 and 18,500. Aluminum fluoride treatment of myelin had the same stimulatory effects on phosphodiesterase activity as did the guanine nucleotides.
Boulias
C
C
Hospital for Sick Children, Department of Biochemistry, Toronto, Ontario, Canada.
Moscarello
M A
MA
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000535:Aluminum; D017607:Aluminum Compounds; D002462:Cell Membrane / Q000378:metabolism; D005459:Fluorides; D006150:Guanine Nucleotides / Q000494:pharmacology*; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000031:analogs & derivatives*; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D006801:Humans; D006868:Hydrolysis; D009185:Myelin Proteins / Q000378:metabolism; D009186:Myelin Sheath / Q000378:metabolism*; D019269:Phosphatidylinositol 4,5-Diphosphate; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010716:Phosphatidylinositols / Q000378:metabolism*; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D013873:Thionucleotides / Q000494:pharmacology; D014316:Tritium
D017607:Aluminum Compounds; D006150:Guanine Nucleotides; D009185:Myelin Proteins; D019269:Phosphatidylinositol 4,5-Diphosphate; D010716:Phosphatidylinositols; D013873:Thionucleotides; C034882:guanosine 5'-O-(1-thiotriphosphate); D014316:Tritium; D000246:Adenosine Diphosphate Ribose; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate; D000535:Aluminum; D010727:Phosphoric Diester Hydrolases; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D005459:Fluorides; C032311:aluminum fluoride