Regulatory elements involved in the tissue-specific expression of the yellow gene of Drosophila.
We have assessed the DNA sequence requirements for the correct spatial pattern and phenotypic expression of y in the late embryo/larvae. The wild-type larval phenotype requires both the regions between -294 bp and -92 bp and a portion of the intron; the sequence element(s) located within the intron can act in a position independent manner to effect the wild-type larval phenotype. The larval expression pattern was examined by tissue experiments in situ and by staining germline transformants derived from various y/lacZ fusion constructs. The larval expression of y is restricted to the mouthparts, microsetae and anal plates. While the -495 bp to +194 bp region alone cannot effect a wild-type larval expression pattern, this region in conjunction with the intron appears to be sufficient to drive beta-gal expression in an essentially wild-type pattern. Our data further suggest that the -294 bp to -92 bp region contains elements which specify the larval pattern and that the element(s) in the intron normally act to enhance the level of expression necessary for the wild-type larval phenotype. We also present a phenotypic analysis of the adult cuticle structures of germline transformants derived from a variety of deletion and rearrangement constructs of the y gene. This analysis has revealed several new features associated with the regulation of y expression.
Martin
M
M
Department of Biochemistry, School of Medical Sciences, University of Bristol, UK.
Meng
Y B
YB
Chia
W
W
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004330:Drosophila / Q000235:genetics* / Q000254:growth & development; D029721:Drosophila Proteins; D005786:Gene Expression Regulation; D007301:Insect Hormones / Q000096:biosynthesis / Q000235:genetics*; D007438:Introns; D007763:Lac Operon; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014170:Transformation, Genetic; D001616:beta-Galactosidase / Q000378:metabolism
D004251:DNA Transposable Elements; D029721:Drosophila Proteins; D007301:Insect Hormones; C050680:y protein, Drosophila; D001616:beta-Galactosidase