title: Organization of the yeast URA2 gene: identification of a defective dihydroorotase-like domain in the multifunctional carbamoylphosphate synthetase-aspartate transcarbamylase complex.
abstract: The 6636 bp of the yeast URA2 gene encoding the carbamoylphosphate synthetase-aspartate transcarbamylase complex have been sequenced. The protein is organized into four regions, three of which are functional domains as indicated previously by genetic analysis. The fourth domain corresponds to a defective dihydroorotase called DHOase-like. The URA2 gene complex with the same organization as the equivalent genes in higher eukaryotes suggests an evolution from a common ancestral gene.
authors.affiliation: Laboratoire de Génétique Physiologique, Institut de Biologie Moléculaire et Cellulaire du C.N.R.S., Strasbourg, France.
authors.forename: J L
authors.initials: JL
authors.lastname: Souciet
authors.forename: M
authors.initials: M
authors.lastname: Nagy
authors.forename: M
authors.initials: M
authors.lastname: Le Gouar
authors.forename: F
authors.initials: F
authors.lastname: Lacroute
authors.forename: S
authors.initials: S
authors.lastname: Potier
mesh_terms: D000581:Amidohydrolases / Q000235:genetics*; D000595:Amino Acid Sequence; D001221:Aspartate Carbamoyltransferase / Q000235:genetics*; D001483:Base Sequence; D002223:Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics; D004080:Dihydroorotase / Q000235:genetics*; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics*; D012331:RNA, Fungal / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic
chemical_list: D004271:DNA, Fungal; D009097:Multienzyme Complexes; D012331:RNA, Fungal; D012333:RNA, Messenger; C019820:carbamoyl phosphate synthase-aspartatecarbamoyl transferase complex; D001221:Aspartate Carbamoyltransferase; D000581:Amidohydrolases; D004080:Dihydroorotase; D002223:Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)