The Oct-1 homoeodomain directs formation of a multiprotein-DNA complex with the HSV transactivator VP16.
The herpes simplex virus transactivator VP16 participates in the formation of a multiprotein-DNA complex with the ubiquitous octamer-motif-binding factor Oct-1. Complex formation is dependent on specific amino acids in the Oct-1 homoeodomain which are in positions analogous to positive control mutations in helix 2 of the lambda phage repressor helix-turn-helix motif, indicating that this structure is an ancient target for protein-protein interactions mediating transcriptional control.
Stern
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Cold Spring Harbor Laboratory, New York 11724.
Tanaka
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Herr
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D000595:Amino Acid Sequence; D004268:DNA-Binding Proteins / Q000378:metabolism* / Q000502:physiology*; D015967:Gene Expression Regulation, Viral; D005801:Genes, Homeobox; D051863:Host Cell Factor C1; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes; D050811:Octamer Transcription Factor-1; D050813:Octamer Transcription Factor-2; D010750:Phosphoproteins / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D018139:Simplexvirus / Q000235:genetics*; D013329:Structure-Activity Relationship; D015534:Trans-Activators / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism* / Q000502:physiology* / Q000648:ultrastructure
D004268:DNA-Binding Proteins; D051863:Host Cell Factor C1; D046911:Macromolecular Substances; D046912:Multiprotein Complexes; D050811:Octamer Transcription Factor-1; D050813:Octamer Transcription Factor-2; C058435:POU2F2 protein, human; D010750:Phosphoproteins; D015534:Trans-Activators; D014157:Transcription Factors