title: Cloning and characterization of the Escherichia coli phosphoglycerate kinase (pgk) gene.
abstract: The pgk gene of Escherichia coli coding for the phosphoglycerate kinase was subcloned from the Carbon and Clarke collection plasmid pLC33-5. The position and direction of transcription of the pgk gene was determined by Tn5 insertion mutagenesis. Analysis of proteins encoded from these plasmids showed that the pgk gene product is a 40-kDa protein, and that the gene is transcribed from two promoters, one immediately in front of the gene and one in front of an upstream gene coding for a 38-kDa polypeptide of unknown function. The position of the Pgk protein on two-dimensional O'Farrel gels was identified, and from this we conclude that it is one of the proteins induced by anaerobiosis [Smith and Neidhardt, J. Bacteriol. 154 (1987) 336-343]. The pgk gene was also found to show growth phase regulation; the synthesis of Pgk protein was induced more than ten-fold during transition from the exponential to the stationary growth phase.
authors.affiliation: Department of Microbiology, Technical University of Denmark, Lyngby.
authors.forename: L J
authors.initials: LJ
authors.lastname: Nellemann
authors.forename: F
authors.initials: F
authors.lastname: Holm
authors.forename: T
authors.initials: T
authors.lastname: Atlung
authors.forename: F G
authors.initials: FG
authors.lastname: Hansen
mesh_terms: D000693:Anaerobiosis; D002453:Cell Cycle; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D009154:Mutation; D010735:Phosphoglycerate Kinase / Q000096:biosynthesis / Q000235:genetics*; D010957:Plasmids; D015183:Restriction Mapping; D014158:Transcription, Genetic
chemical_list: D004251:DNA Transposable Elements; D010735:Phosphoglycerate Kinase