Structure and function of hot spots providing signals for site-directed specific recombination and gene expression in Tn21 transposons.
Tn21- and Tn3-related transposons are widespread and carry various resistance determinants. The insertion points of different resistance genes were precisely defined in Tn2424, Tn1696, Tn2410, Tn4000 and its derivatives and compared to the corresponding sites in Tn7, pSA, R388, R46, Tn2603, Tn1331 and in Tn3-related elements. Insertional 'hot spots' located at the 3' end of different genes comprised 55 nucleotides and yielded more than 90% homology to the corresponding consensus sequence, termed hs1. Elements of this class were found to direct recA-independent generation of deletions. Flanking the 5' ends, hs2 (CTAAAACAAAGTTA) comprised the terminal nucleotides of hs1. Functional properties of hot spots as recognition sites for site-specific recombination and regulation of gene expression indicate that they might be involved in transfer, stable inheritance and expression of prokaryotic genes.
Schmidt
F R
FR
Institut für Bodenbiologie, Bundesforschungsanstalt für Landwirtschaft, Braunschweig.
Nücken
E J
EJ
Henschke
R B
RB
D001483:Base Sequence; D002872:Chromosome Deletion; D004251:DNA Transposable Elements; D004352:Drug Resistance, Microbial / Q000235:genetics*; D015870:Gene Expression; D008969:Molecular Sequence Data; D011995:Recombination, Genetic; D012689:Sequence Homology, Nucleic Acid; D013302:Streptomyces / Q000235:genetics; D013329:Structure-Activity Relationship; D014162:Transfection
D004251:DNA Transposable Elements