title: [Modification of human DNA polymerase alpha by N-acetylimidazole].
abstract: The modification of tyrosine residues of the human placenta DNA-polymerase alpha by N-acetylimidazole was investigated. The poly(dT)-template and the r(pA)10-primer a each added separately or simultaneously do not influence the rate of enzyme inactivation. In the presence of poly(dT)-r(pA)10 no effect of dCTP and dTTP (noncomplementary to template) and of dAMP and dADP (complementary to template) on the rate and the level of the enzyme inactivation was found. However dATP revealed practically complete protection. Orthophosphate, pyrophosphate each taken separately do not influence the rate of enzyme inactivation with this reagent. The presence of dADP with either ortho- or pyrophosphate, or dAMP with the one of these ligands leads to half protective action in comparison with dATP. Imidazolides of phosphonoacetic acid and 5'-adenylyl++ 1(phosphonoacetic acid) do not inactivate DNA-polymerase alpha from human placenta and the Klenov fragment of DNA-polymerase I from E. coli. All data obtained allow to suggest that the tyrosine residue in the dNTP binding site of DNA-polymerase reveals stacking with the nucleotide only if dNTP is complementary to the template.
authors.forename: O I
authors.initials: OI
authors.lastname: Lavrik
authors.forename: G A
authors.initials: GA
authors.lastname: Nevinskiĭ
authors.forename: I A
authors.initials: IA
authors.lastname: Potapova
authors.forename: N B
authors.initials: NB
authors.lastname: Tarusova
authors.forename: O V
authors.initials: OV
authors.lastname: Khalabuda
mesh_terms: D004257:DNA Polymerase II / Q000378:metabolism*; D003838:Deoxyadenine Nucleotides; D011756:Diphosphates; D004926:Escherichia coli / Q000201:enzymology; D005260:Female; D006801:Humans; D007093:Imidazoles; D007700:Kinetics; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy; D013439:Sulfhydryl Reagents; D014443:Tyrosine / Q000378:metabolism
chemical_list: D003838:Deoxyadenine Nucleotides; D011756:Diphosphates; D007093:Imidazoles; D013439:Sulfhydryl Reagents; D014443:Tyrosine; D004257:DNA Polymerase II; C012160:N-acetylimidazole
vernacular_title: Modifikatsiia DNK-polimerazy alpha cheloveka N-atsetilimidazolom.