Production of diarrhea in the rabbit by a mutant of Escherichia coli (RDEC-1) that does not express adherence (AF/R1) pili.
Escherichia coli (RDEC-1) adheres to Peyer's patch and absorptive epithelium in the rabbit in the closely adhering manner characteristic of enteropathogenic and enterohemorrhagic E. coli. An adherence pilus (AF/R1) is important for adherence to Peyer's patch M cells in vivo and ileal brush borders in vitro. A nonpiliated mutant (42-2-37-8) of the RDEC-1 strain colonized the gut lumen less readily than the parent strain. The mutant adhered infrequently to Peyer's patch lymphoid follicle epithelium (22% vs. 84%, P less than .0001). Ileal close adherence was less frequent at 3 d, but by 9 d after inoculation had increased, approaching that of the parent RDEC-1 strain. However, adherence was focal, and fewer bacteria were present at each adherence site compared with the parent RDEC-1 strain. The result was a lower frequency of diarrhea (34% vs. 65%, P = .003) and mortality (9.4% vs. 27%, P = .035) with the 42-2-37-8 strain. Loss of AF/R1 pili compromised the ability of the RDEC-1 strain to adhere to Peyer's patch and absorptive epithelium and to produce diarrhea.
Cantey
J R
JR
Veterans Administration Medical Center, Charleston, SC 29403.
Inman
L R
LR
Blake
R K
RK
D000818:Animals; D001422:Bacterial Adhesion; D003967:Diarrhea / Q000382:microbiology*; D004848:Epithelium / Q000382:microbiology; D004926:Escherichia coli / Q000235:genetics / Q000472:pathogenicity* / Q000648:ultrastructure; D004927:Escherichia coli Infections / Q000382:microbiology*; D010861:Fimbriae, Bacterial; D007422:Intestines / Q000382:microbiology; D008854:Microscopy, Electron; D009154:Mutation; D010581:Peyer's Patches / Q000382:microbiology; D011817:Rabbits