Purification of 25-hydroxyvitamin D3 24-hydroxylase from rat kidney mitochondria.
25-Hydroxyvitamin D3 24-hydroxylase was purified to an electrophoretically homogeneous state (Mr = 53,000) from kidney mitochondria of female rats treated with vitamin D3.
Ohyama
Y
Y
Department of Biochemistry, Hiroshima University School of Dentistry, Japan.
Hayashi
S
S
Okuda
K
K
D000818:Animals; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003577:Cytochrome P-450 Enzyme System; D017886:Durapatite; D006882:Hydroxyapatites; D007425:Intracellular Membranes / Q000201:enzymology; D007668:Kidney / Q000201:enzymology*; D007700:Kinetics; D008928:Mitochondria / Q000201:enzymology*; D051381:Rats; D013250:Steroid Hydroxylases / Q000302:isolation & purification* / Q000378:metabolism; D013367:Submitochondrial Particles / Q000201:enzymology; D065668:Vitamin D3 24-Hydroxylase
D006882:Hydroxyapatites; D003577:Cytochrome P-450 Enzyme System; D017886:Durapatite; D013250:Steroid Hydroxylases; D065668:Vitamin D3 24-Hydroxylase