Synthesis of a gene for the protein kinase domain of the epidermal growth factor receptor and its expression in Escherichia coli.
A gene encoding the protein kinase domain of the epidermal growth factor receptor has been chemically synthesised, cloned and expressed in Escherichia coli. The 942-base-pair gene was constructed by enzymatic ligation of 56 oligonucleotides and cloned into an expression vector downstream of the E. coli trp promoter. Production of active gene product was confirmed by means of a protein kinase assay, demonstrating that the enzymatic activity of the protein kinase domain of the epidermal growth factor receptor is retained after expression in E. coli.
Farrow
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National Cancer Center Research Institute, Biology Division, Tokyo.
Kamiya
H
H
Miura
K
K
Ohtsuka
E
E
Nishimura
S
S
D000595:Amino Acid Sequence; D001482:Base Composition; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D066246:ErbB Receptors / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D015870:Gene Expression; D005796:Genes; D005813:Genes, Synthetic; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D011505:Protein-Tyrosine Kinases / Q000235:genetics* / Q000378:metabolism
D066246:ErbB Receptors; D011505:Protein-Tyrosine Kinases