Isolation and characterization of temperature-sensitive mutants of Abelson murine leukaemia virus that exhibit dissociation among morphological transformation, soft agar colony-forming ability and tyrosine kinase activity.
Seven temperature-sensitive (ts) mutants of Abelson murine leukaemia virus (A-MuLV) were isolated on the basis of the temperature dependence of their soft agar colony-forming ability. These seven ts mutants exhibited similar characteristics and were not ts for morphological transformation and autophosphorylation of P120gag-abl protein. The dissociation of the properties of morphology, soft agar colony formation and tyrosine kinase activity might suggest that the v-abl product has more than one primary intracellular target.
Oka
Y
Y
Third Department of Internal Medicine, Osaka University Medical School, Japan.
Sugiyama
H
H
Tsukada
S
S
Shimizu
Y
Y
Inoue
H
H
Hakura
A
A
Kishimoto
S
S
D000011:Abelson murine leukemia virus / Q000201:enzymology / Q000235:genetics* / Q000502:physiology; D000362:Agar; D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D009052:Leukemia Virus, Murine / Q000235:genetics*; D051379:Mice; D009154:Mutation; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D013696:Temperature
D000362:Agar; D011505:Protein-Tyrosine Kinases