Human myeloid plasma membrane glycoprotein CD13 (gp150) is identical to aminopeptidase N.
To determine the primary structure of CD13, a 150-kD cell surface glycoprotein originally identified on subsets of normal and malignant human myeloid cells, we isolated the complete sequences encoding the polypeptide in overlapping complementary DNA (cDNA) clones. The authenticity of our cDNA clones was demonstrated by the ability of the coding sequences, subcloned in a retroviral expression vector, to mediate expression of bona fide CD13 molecules at the surface of transfected mouse fibroblasts. The nucleotide sequence predicts a 967 amino acid integral membrane protein with a single, 24 amino acid hydrophobic segment near the amino terminus. Amino-terminal protein sequence analysis of CD13 molecules indicated that the hydrophobic segment is not cleaved, but rather serves as both a signal for membrane insertion and as a stable membrane-spanning segment. The remainder of the molecule consists of a large extracellular carboxyterminal domain, which contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloprotease superfamily. Sequence comparisons with known enzymes of this class revealed that CD13 is identical to aminopeptidase N, a membrane-bound glycoprotein thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes prepared from cells of the central nervous system.
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Department of Hematology-Oncology, St. Jude Children's Research Hospital, Memphis, Tennessee 38101.
Ashmun
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Shapiro
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Peiper
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SC
D000595:Amino Acid Sequence; D000626:Aminopeptidases / Q000235:genetics / Q000302:isolation & purification*; D015214:Antigens, Differentiation, Myelomonocytic / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D018826:CD13 Antigens; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D005822:Genetic Vectors; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D011487:Protein Conformation; D012191:Retroviridae Proteins / Q000235:genetics
D015214:Antigens, Differentiation, Myelomonocytic; D008562:Membrane Glycoproteins; D012191:Retroviridae Proteins; D004247:DNA; D000626:Aminopeptidases; D018826:CD13 Antigens