Quantification of the mass of tyrosine monooxygenase in the median eminence and superior cervical ganglion.
A procedure is described that enables one to quantify the mass of TH in a fraction of the ME and SCG of rats. This procedure is specific and sensitive. It should be possible to study the biosynthetic activity of catecholaminergic neurons as a function of the mass of TH in such cells.
Porter
J C
JC
Wang
P S
PS
Kedzierski
W
W
Gonzalez
H A
HA
D000310:Adrenal Gland Neoplasms / Q000201:enzymology; D000818:Animals; D002460:Cell Line; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005260:Female; D005728:Ganglia, Sympathetic / Q000201:enzymology*; D007106:Immune Sera; D015151:Immunoblotting / Q000379:methods; D007202:Indicators and Reagents; D008473:Median Eminence / Q000201:enzymology*; D008970:Molecular Weight; D010673:Pheochromocytoma / Q000201:enzymology; D051381:Rats; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism
D007106:Immune Sera; D007202:Indicators and Reagents; D014446:Tyrosine 3-Monooxygenase