Productive ascites growth of heterohybridomas between Epstein-Barr virus-transformed human B cells and murine P3X63Ag8.653 myeloma cells.
Heterohybridomas between Epstein-Barr virus-transformed human B cells and murine myeloma P3X63Ag8.653 cells were found to produce sizable quantities of human monoclonal antibodies in nude mouse ascites. Mice injected intraperitoneally with two of such heterohybridomas yielded several milliters of ascites fluid which contained nearly 100-fold higher anti-platelet activities than those in routine culture supernatants.
Matsumoto
M
M
First Department of Internal Medicine, School of Medicine, Ehime University, Shigenobu.
Mochizuki
K
K
Kobayashi
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D001201:Ascites / Q000382:microbiology; D001402:B-Lymphocytes; D002472:Cell Transformation, Viral; D005260:Female; D004854:Herpesvirus 4, Human; D006801:Humans; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009101:Multiple Myeloma / Q000276:immunology
D000911:Antibodies, Monoclonal