Rat brain capillary endothelial cells express functional PDGF B-type receptors.
Immunohistochemical staining revealed the presence of platelet-derived growth factor (PDGF) B-type receptors on capillaries of normal rat brain. Furthermore, capillary endothelial cells isolated from rat brain and grown in tissue culture bound [125I]PDGF-BB but not [125I]PDGF-AA, suggesting that they expressed B-type, but not A-type, PDGF receptors. PDGF-BB and PDGF-AB, but not PDGF-AA, also stimulated incorporation of [3H]thymidine into these cells. Thus, rat brain capillary endothelial cells have functional B-type receptors, and thereby differ from endothelial cells derived from large blood vessels, that do not express PDGF receptors. Our data suggest a possible role for PDGF-BB as an angiogenic factor.
Smits
A
A
Ludwig Institute for Cancer Research, Biomedical Center, Uppsala, Sweden.
Hermansson
M
M
Nistér
M
M
Karnushina
I
I
Heldin
C H
CH
Westermark
B
B
Funa
K
K
D043925:Angiogenesis Inducing Agents / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000098:blood supply*; D002196:Capillaries / Q000187:drug effects / Q000378:metabolism; D046508:Culture Techniques; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D007150:Immunohistochemistry; D010982:Platelet-Derived Growth Factor / Q000145:classification / Q000378:metabolism* / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000145:classification / Q000187:drug effects / Q000378:metabolism*; D017479:Receptors, Platelet-Derived Growth Factor; D013936:Thymidine / Q000378:metabolism
D043925:Angiogenesis Inducing Agents; D010982:Platelet-Derived Growth Factor; D011956:Receptors, Cell Surface; D017479:Receptors, Platelet-Derived Growth Factor; D013936:Thymidine