Detection of herpes simplex virus by using A549 cells in centrifugation culture with a rapid membrane enzyme immunoassay.
The RAMP herpes simplex virus (HSV) culture confirmation test was compared with immunofluorescence (IF) staining with a specific HSV monoclonal antibody reagent for the detection of HSV in centrifugation culture. The RAMP test detected 47 of 57 IF-positive specimens (sensitivity, 88.6%) and agreed with 217 of 220 IF-negative specimens (specificity, 98.6%). The RAMP test can be performed in less than 15 min and gives an immediate visual result. However, the sensitivity and the false-positive and false-negative results need further investigation.
Hursh
D A
DA
Diagnostic Virology Laboratory, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
Wendt
S F
SF
Lee
C F
CF
Gleaves
C A
CA
D002460:Cell Line; D002498:Centrifugation; D003588:Cytopathogenic Effect, Viral; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D018139:Simplexvirus / Q000302:isolation & purification*
D011933:Reagent Kits, Diagnostic