title: Autoradiographic distribution of thyrotropin-releasing hormone receptors in the African lungfish Protopterus annectens.
abstract: We used quantitative autoradiography to examine the distribution of thyrotropin-releasing hormone (TRH) receptors in the central nervous system (CNS) of the African lungfish Protopterus annectens. We found that the distribution of TRH receptors throughout the CNS of the lungfish was heterogeneous with the highest concentrations (500-800 fmol/mg protein) in the olfactory bulb and telencephalon, moderately high concentrations (200-500 fmol/mg protein) in the diencephalon, and moderate (50-200 fmol/mg protein) to low (less than 50 fmol/mg protein) concentrations in the brainstem and spinal cord. Except for the motor nuclei of the cranial nerves and spinal cord, TRH receptors were concentrated in the acellular regions. In the telencephalon and diencephalon, the receptor density was inversely related to cellular density. These results provide a neuroanatomic and neuropharmacologic basis for further investigations of TRH in the African lungfish.
authors.affiliation: Department of Medicine, University of Pennsylvania, Philadelphia 19104-4283.
authors.forename: A M
authors.initials: AM
authors.lastname: Pack
authors.forename: S B
authors.initials: SB
authors.lastname: Caine
authors.forename: A
authors.initials: A
authors.lastname: Winokur
authors.forename: S
authors.initials: S
authors.lastname: Manaker
authors.forename: A P
authors.initials: AP
authors.lastname: Fishman
mesh_terms: D000818:Animals; D001345:Autoradiography; D002490:Central Nervous System / Q000033:anatomy & histology / Q000378:metabolism*; D004027:Diencephalon / Q000033:anatomy & histology / Q000378:metabolism; D005399:Fishes / Q000378:metabolism*; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008845:Microinjections; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D017981:Receptors, Neurotransmitter / Q000032:analysis*; D018025:Receptors, Thyrotropin-Releasing Hormone; D013687:Telencephalon / Q000033:anatomy & histology / Q000378:metabolism; D013973:Thyrotropin-Releasing Hormone / Q000031:analogs & derivatives
chemical_list: D017981:Receptors, Neurotransmitter; D018025:Receptors, Thyrotropin-Releasing Hormone; C014109:thyrotropin-releasing hormone, 3-Me-; D013973:Thyrotropin-Releasing Hormone; D011761:Pyrrolidonecarboxylic Acid