Antigen-presenting capacity of epidermal cells infected with vaccinia virus recombinants containing the herpes simplex virus glycoprotein D, and protective immunity.
We studied the association of herpes simplex type 1 (HSV-1) glycoprotein D (gD-1) expression in epidermal cells (EC) with virus-specific immunity and protection of mice from fatal HSV-2 challenge. Vaccinia virus recombinants containing gD-1 under the control of an early (VP176) or late (VP254) vaccinia virus promoter were used. Mature gD-1 protein was expressed in VP176-infected EC and they had accessory cell function for HSV-2-induced T cell proliferation of immune lymph node cells (LNC). It was not expressed in VP254-infected EC and they did not act as accessory cells. LNC from VP176- but not VP254-immunized mice proliferated in response to HSV antigen and only VP176-immunized mice had complete long-term protection from HSV-2 challenge.
Wachsman
M
M
Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, Baltimore 21201.
Luo
J H
JH
Aurelian
L
L
Perkus
M E
ME
Paoletti
E
E
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology / Q000382:microbiology*; D002460:Cell Line; D004817:Epidermis / Q000276:immunology / Q000382:microbiology*; D005260:Female; D006801:Humans; D007109:Immunity; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D018139:Simplexvirus / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity; D014616:Vaccinia virus / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000235:genetics*
D014759:Viral Envelope Proteins; C039447:glycoprotein D, Human herpesvirus 1