title: Defective phosphorylation of a calmodulin-binding protein in cystic-fibrosis submandibular glands.
abstract: Calmodulin-binding proteins in fractions purified from human submandibular glands by calmodulin-Sepharose were phosphorylated with [gamma-32P]ATP, in the absence of exogenous protein kinase. The major proteins phosphorylated had molecular masses of 45, 51 and 61 kDa. Phosphorylation was increased by activators of protein kinase C and inhibited by H-7. Phosphorylation of the 61 kDa band was markedly decreased in cystic-fibrosis submandibular glands.
authors.affiliation: Department of Medical Biochemistry, University of Wales College of Medicine, Cardiff, U.K.
authors.forename: D K
authors.initials: DK
authors.lastname: Shori
authors.forename: R L
authors.initials: RL
authors.lastname: Dormer
authors.forename: M C
authors.initials: MC
authors.lastname: Goodchild
authors.forename: M A
authors.initials: MA
authors.lastname: McPherson
mesh_terms: D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000255:Adenosine Triphosphate / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D019703:Calcineurin; D002148:Calmodulin-Binding Proteins / Q000378:metabolism*; D000242:Cyclic AMP / Q000494:pharmacology; D003550:Cystic Fibrosis / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D007546:Isoquinolines / Q000494:pharmacology; D010749:Phosphoprotein Phosphatases / Q000378:metabolism; D010766:Phosphorylation; D010879:Piperazines / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism; D013363:Submandibular Gland / Q000378:metabolism*; D014268:Trifluoperazine / Q000494:pharmacology
chemical_list: D002148:Calmodulin-Binding Proteins; D007546:Isoquinolines; D010879:Piperazines; D014268:Trifluoperazine; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000255:Adenosine Triphosphate; D000242:Cyclic AMP; D011493:Protein Kinase C; D019703:Calcineurin; D010749:Phosphoprotein Phosphatases