The translational requirement for complete La Crosse virus mRNA synthesis is cell-type dependent.
The translational requirement to prevent premature termination during La Crosse virus S mRNA synthesis was found to be cell-type dependent. This requirement was present in the BHK, HEL, and Vero cell lines we examined, but not in C6/36 mosquito cells. The cell-dependent translational requirement could be reproduced in vitro by using either cell extracts or purified virions of BHK and C6/36 cells. In the BHK reactions, the polymerase terminated predominantly at nucleotide 175 in the absence of concurrent translation and required translation to read through this position. In the C6/36 reactions, however, the polymerase reads through nucleotide 175 efficiently independent of translation. Reconstitution studies suggested that the translational requirement was due to a factor(s) present in BHK, but not in C6/36, cells.
Raju
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Department of Microbiology, University of Geneva School of Medicine, Switzerland.
Raju
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Kolakofsky
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D000818:Animals; D002043:Bunyaviridae / Q000235:genetics*; D002460:Cell Line; D009033:Culicidae; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D002141:Encephalitis Virus, California / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis*; D011817:Rabbits; D014158:Transcription, Genetic; D014709:Vero Cells; D014771:Virion / Q000235:genetics / Q000378:metabolism
D012333:RNA, Messenger; D012321:DNA-Directed RNA Polymerases