Evidence for a phosphatidylinositol anchor in glycolipid antigens of Leishmania major.
The structure of the membrane anchor of three Leishmania major surface antigenic glycolipids was analyzed. Phosphatidylinositol-specific phospholipase C treatment and nitrous acid deamination indicated the presence of a phosphatidylinositol anchor linked to the glycan through a non-N-acetylated hexosamine. An ester linkage on the C-2 of glycerol was revealed by phospholipase A2 hydrolysis. This fatty acyl substitution was not found on the phosphatidylinositol anchor of the Leishmania lipophosphoglycan.
Sevlever
D
D
Department of Parasitology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Londner
M V
MV
Rosen
G
G
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D002845:Chromatography; D006017:Glycolipids / Q000032:analysis* / Q000276:immunology; D007895:Leishmania tropica / Q000276:immunology*; D009608:Nitrous Acid; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010716:Phosphatidylinositols / Q000032:analysis*; D054801:Phosphoinositide Phospholipase C; D010741:Phospholipases A; D054467:Phospholipases A2; D010727:Phosphoric Diester Hydrolases
D000953:Antigens, Protozoan; D000954:Antigens, Surface; D006017:Glycolipids; D010716:Phosphatidylinositols; D010741:Phospholipases A; D054467:Phospholipases A2; D010727:Phosphoric Diester Hydrolases; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D009608:Nitrous Acid