Influence of several experimental parameters on inter and intra specific mating process efficiency.
In this study we investigated the influence that several experimental parameters may product on the mating process efficiency. We used the ordinary mating technique described by other authors and we examined the effect of temperature, media and plasmid size on the number of recipient cells transformed by mating procedure. The results showed that the most important parameter influencing the efficiency of the mating transformation is the media, associated with the size of the plasmid to be transferred.
Sambri
V
V
Institute of Microbiology, University of Bologna, S. Orsola Hospital, Italy.
Lovett
M A
MA
D003227:Conjugation, Genetic; D003470:Culture Media; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D011815:R Factors / Q000235:genetics*; D012475:Salmonella / Q000235:genetics*; D013696:Temperature; D014169:Transformation, Bacterial
D003470:Culture Media; D004251:DNA Transposable Elements