Sorbitol-gelatin and glutamic acid-lactose solutions for stabilization of reference preparations of measles virus.
The work reported here sought to assess the protection afforded by two stabilizing solutions (sorbitol-gelatin and glutamic acid-lactose) in preserving the potency of freeze-dried Schwarz strain measles virus during storage with a view to the production of reference preparations and working lots of virus suspensions. Stabilized virus suspensions and control suspensions were stored at -70 degrees C or were freeze-dried and stored at -20 degrees C, and their potency was determined over a storage period of 21 months. It was found that the sorbitol-gelatin imparted more satisfactory stability (r = +0.18) to the freeze-dried virus suspensions than did the glutamic acid-lactose. The results also indicate that sorbitol-gelatin, used under the conditions of this study, is an effective stabilizer in the preparation of freeze-dried suspensions of Schwarz strain measles virus employed as reference preparation working lots.
de Rizzo
E
E
Tenório
E C
EC
Mendes
I F
IF
Fang
F L
FL
Pral
M M
MM
Takata
C S
CS
Miyaki
C
C
Gallina
N M
NM
Tuchiya
H N
HN
Akimura
O K
OK
D003470:Culture Media; D005612:Freeze Drying; D005780:Gelatin; D005971:Glutamates; D018698:Glutamic Acid; D006801:Humans; D007785:Lactose; D008458:Measles Vaccine / Q000592:standards*; D008459:Measles virus / Q000502:physiology*; D012015:Reference Standards; D013012:Sorbitol; D014613:Vaccines, Attenuated / Q000592:standards; D014776:Virus Cultivation / Q000379:methods*
D003470:Culture Media; D005971:Glutamates; D008458:Measles Vaccine; D014613:Vaccines, Attenuated; D018698:Glutamic Acid; D013012:Sorbitol; D005780:Gelatin; D007785:Lactose