Human teratocarcinoma cells express functional insulin-like growth factor I receptors.
Using iodinated insulin-like growth factors (IGFs) we have detected receptors for IGF-I at the cell surface of the clonally derived human embryonal carcinoma cell line Tera 2 clone 13. Affinity crosslinking of IGFs to Tera 2 clone 13-derived membrane preparations revealed the presence of proteins with features of both type-I and type-II IGF receptors. Treatment of Tera 2 clone 13 cells with retinoic acid to induce differentiation results in an increased number of cell surface receptors, apparently without altering the ratio of type-I and type-II receptors. In addition, Tera 2 clone 13 IGF-I receptors catalyze (auto)phosphorylation at tyrosine upon IGF-I and insulin binding. These findings suggest that type-I IGF receptors might be involved in mediating the effects of IGFs and insulin upon the proliferation of Tera 2 clone 13 cells.
Weima
S M
SM
Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Utrecht.
Stet
L H
LH
van Rooijen
M A
MA
van Buul-Offers
S C
SC
van Zoelen
E J
EJ
de Laat
S W
SW
Mummery
C L
CL
D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D015870:Gene Expression; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D007335:Insulin-Like Growth Factor II / Q000494:pharmacology; D009373:Neoplasms, Germ Cell and Embryonal / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure*; D010770:Phosphotransferases / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics / Q000502:physiology*; D017451:Receptors, Somatomedin; D013724:Teratoma / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure*; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000473:pathology
D007328:Insulin; D011956:Receptors, Cell Surface; D017451:Receptors, Somatomedin; D014212:Tretinoin; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II; D010770:Phosphotransferases