Nuclear envelope assembly around sperm chromatin in cell-free preparations from Drosophila embryos.
Chicken sperm chromatin initiated an assembly of interphase-like nuclei in a cell-free cytoplasmic preparation from 1-6 h old Drosophila melanogaster embryos. The formation of these interphase-like nuclei from the condensed sperm chromatin happened in a series of distinct steps. Anti-Drosophila lamin monoclonal antibody stained the assembled nuclei in a pattern indistinguishable from normal Drosophila nuclei. This assembly process required an ATP regenerating system and could be blocked by the addition of novobiocin into the cell-free extract.
Ulitzur
N
N
Department of Genetics, Hebrew University of Jerusalem, Israel.
Gruenbaum
Y
Y
D000255:Adenosine Triphosphate / Q000502:physiology; D000818:Animals; D002467:Cell Nucleus / Q000502:physiology* / Q000648:ultrastructure; D002474:Cell-Free System; D002645:Chickens; D002843:Chromatin / Q000502:physiology*; D004250:DNA Topoisomerases, Type II / Q000502:physiology; D004331:Drosophila melanogaster; D066298:In Vitro Techniques; D034882:Lamins; D008297:Male; D009024:Morphogenesis; D009675:Novobiocin / Q000494:pharmacology; D009685:Nuclear Envelope / Q000502:physiology* / Q000648:ultrastructure; D009687:Nuclear Proteins / Q000378:metabolism; D013094:Spermatozoa / Q000648:ultrastructure*
D002843:Chromatin; D034882:Lamins; D009687:Nuclear Proteins; D009675:Novobiocin; D000255:Adenosine Triphosphate; D004250:DNA Topoisomerases, Type II