title: The products of the reduction of doxyl stearates in cells are hydroxylamines as shown by oxidation by 15N-perdeuterated Tempone.
abstract: The use of nitroxides in functional biological systems has increased greatly as it has become evident that such studies can provide valuable biophysical and metabolic data. This has led to a need to understand the nature of the metabolism of nitroxides and their products. This paper presents data indicating the value of 15N-perdeuterated Tempone specifically to indicate the amount of hydroxylamines that are present in a cellular system. Using this technique, we found that in the mammalian cells that we studied the principal or only products of reduction of doxyl stearates were the corresponding hydroxylamines.
authors.affiliation: Department of Physiology and Biophysics College of Medicine, University of Illinois, Urbana.
authors.forename: K
authors.initials: K
authors.lastname: Chen
authors.forename: H M
authors.initials: HM
authors.lastname: Swartz
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000032:analysis; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003497:Cyclic N-Oxides / Q000378:metabolism*; D003903:Deuterium; D004578:Electron Spin Resonance Spectroscopy; D006898:Hydroxylamines / Q000032:analysis*; D051379:Mice; D010084:Oxidation-Reduction; D013950:Thymus Gland / Q000032:analysis / Q000166:cytology; D014216:Triacetoneamine-N-Oxyl
chemical_list: D003497:Cyclic N-Oxides; D006898:Hydroxylamines; D014216:Triacetoneamine-N-Oxyl; C044761:10-doxylstearic acid; C032363:16-nitroxystearic acid; D003903:Deuterium