Cloning and characterization of variable-sized gypsy mobile elements in Drosophila melanogaster.
A cosmid genomic library from a known gypsy-induced forked mutation, f1, was screened by 32P-labeled gypsy transposable element. Of more than 250 positive clones we randomly selected 21 for in situ hybridization to wild-type polytene chromosomes. Two clones hybridized to region 15F on the X-chromosome, the cytological position of forked. A third clone hybridized to at least 17 sites on the chromosomes indicating the presence of repetitive sequences in the gypsy flanking DNA. All clones labeled the centromeric regions heavily. Ten clones, including the two hybridizing at 15F, were chosen for further analysis, and restriction mapping allowed us to place them into three groups: (1) full-length, (2) slightly diverging, and (3) highly diverging gypsy elements. Group (2) is missing the XbaI site in both their long terminal repeats (LTRs) as well as the middle HindIII site; four of these gypsy elements also have a approximately 100-bp deletion at the 5' LTR. The group (3) gypsy transposons are missing one LTR and also have highly diverging DNA sequences. The restriction analyses further imply that most of these different gypsy elements are present in more than one copy in the genome of the f1 stock used in this study. The results raise intriguing questions regarding the significance of transposable elements in evolution and biological functions.
Lambertsson
A
A
Department of Genetics, University of Umeå, Sweden.
Andersson
S
S
Johansson
T
T
D000818:Animals; D015139:Blotting, Southern; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D003360:Cosmids; D004247:DNA / Q000235:genetics*; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics*; D009154:Mutation; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D014960:X Chromosome / Q000378:metabolism
D004251:DNA Transposable Elements; D004247:DNA