title: Fine-structure genetic map of the maltose transport operon of Salmonella typhimurium.
abstract: We have constructed a fine-structure genetic map of the maltose transport operon in Salmonella typhimurium. We have isolated mal mutants by using indicator plates, penicillin selection, or a proton suicide technique. Mutants were obtained as spontaneous events or were induced by chemical mutagenesis and transposon insertion. Tn10 and Mu d(lac Ap)1 insertion mutations were used to create deletions. Mutations were also obtained in a gene that is equivalent to lamB in Escherichia coli, which codes for the lambda bacteriophage receptor. The gene products in the mutants were characterized by sodium dodecyl sulfate-polyacrylamide-gel electrophoresis and immunoblotting. Our data indicate that the location of this operon on the Salmonella chromosome as well as the gene order and its orientation are the same as those in E. coli. This map will be useful in studying the mechanism of periplasmic transport in S. typhimurium.
authors.affiliation: Department of Biochemistry, University of California, Berkeley 94720.
authors.forename: E
authors.initials: E
authors.lastname: Schneider
authors.forename: L
authors.initials: L
authors.lastname: Bishop
authors.forename: E
authors.initials: E
authors.lastname: Schneider
authors.forename: V
authors.initials: V
authors.lastname: Alfandary
authors.forename: G F
authors.initials: GF
authors.lastname: Ames
mesh_terms: D018528:ATP-Binding Cassette Transporters; D002352:Carrier Proteins / Q000235:genetics*; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D004251:DNA Transposable Elements; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D005838:Genotype; D008320:Maltose / Q000378:metabolism*; D058619:Maltose-Binding Proteins; D009004:Monosaccharide Transport Proteins; D009154:Mutation; D009876:Operon; D033902:Periplasmic Binding Proteins; D010957:Plasmids; D015183:Restriction Mapping; D012486:Salmonella typhimurium / Q000235:genetics*
chemical_list: D018528:ATP-Binding Cassette Transporters; D002352:Carrier Proteins; D004251:DNA Transposable Elements; D029968:Escherichia coli Proteins; C461619:MalE protein, E coli; D058619:Maltose-Binding Proteins; D009004:Monosaccharide Transport Proteins; D033902:Periplasmic Binding Proteins; C461599:maltose transport system, E coli; D008320:Maltose