title: Effect of a hyperlipidic diet on lipid composition, fluidity, and (Na+-K+)ATPase activity of rat erythrocyte membranes.
abstract: Feeding rats a hyperlipidic diet in which animals were offered daily a variety of high-energy food resulted in a significant increase of serum free fatty acids and a decrease of phospholipids with respect to controls. On the contrary, there were no significant differences in erythrocyte membrane total lipid composition between the two groups. Erythrocyte membranes showed a significant decrease in saturated fatty acid content and a significant increase in (n-6) polyunsaturated fatty acid content; (n-3) polyunsaturated fatty acids significantly decreased. Membrane fluidity, investigated by fluorescence polarization of diphenylhexatriene, significantly increased in the erythrocyte membranes of the experimental group. These results seem compatible with the decreased saturated/unsaturated fatty acid ratio. A significant decrease of (Na+-K+)ATPase activity occurred in erythrocyte membranes of the experimental group rats with respect to the controls.
authors.affiliation: Dipartimento di Biochimica, Universita' di Bologna, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Bordoni
authors.forename: P L
authors.initials: PL
authors.lastname: Biagi
authors.forename: G
authors.initials: G
authors.lastname: Parenti Castelli
authors.forename: S
authors.initials: S
authors.lastname: Hrelia
authors.forename: C A
authors.initials: CA
authors.lastname: Rossi
authors.forename: G
authors.initials: G
authors.lastname: Lercker
authors.forename: J C
authors.initials: JC
authors.lastname: Izpisua
authors.forename: T
authors.initials: T
authors.lastname: Barber
authors.forename: J
authors.initials: J
authors.lastname: Cabo
authors.forename: G
authors.initials: G
authors.lastname: Lenaz
mesh_terms: D000818:Animals; D002849:Chromatography, Gas; D004032:Diet; D004041:Dietary Fats / Q000494:pharmacology*; D004435:Eating; D004910:Erythrocyte Membrane / Q000201:enzymology / Q000378:metabolism*; D005227:Fatty Acids / Q000097:blood; D008055:Lipids / Q000097:blood*; D008297:Male; D008560:Membrane Fluidity; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000097:blood*
chemical_list: D004041:Dietary Fats; D005227:Fatty Acids; D008055:Lipids; D000254:Sodium-Potassium-Exchanging ATPase