Lymphocyte subpopulation with low membrane potential in the blood of cyclosporin- and prednisone-treated patients: in vivo selectivity for T4 subset.
Previous work on the mode of action of CsA revealed that this drug shifts the membrane potential of human periferal blood lymphocytes in vitro. Recently we have analyzed lymphocytes of cyclosporin-treated transplant patients. Membrane potential analysis with the DIOC6(3) fluorescence dye indicates that all the studied patients have a subpopulation of lymphocytes with low membrane potential and that this population is made up predominently of OKT4+ cells. However there is no correlation between the clinical events and the percentage distribution of lymphocyte subpopulations as defined by the membrane potential and the T4/T8 ratio of the total lymphocyte population.
Aszalos
A
A
Division of Drug Biology, Food and Drug Administration, Washington, D.C. 20204.
Tron
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L
Paxton
H
H
Shen
S
S
D015496:CD4-Positive T-Lymphocytes; D003524:Cyclosporins / Q000097:blood* / Q000627:therapeutic use; D005434:Flow Cytometry; D006801:Humans; D008214:Lymphocytes / Q000145:classification*; D008564:Membrane Potentials / Q000187:drug effects*; D011241:Prednisone / Q000097:blood* / Q000627:therapeutic use
D003524:Cyclosporins; D011241:Prednisone