title: Activation of peripheral blood T cells via the p75 interleukin 2 receptor.
abstract: By using mAb and flow cytometry, a constitutive expression of the p75 IL-2R was revealed in human peripheral blood CD8+ T cells and TCR delta-1+ T cells as well as in CD16+ NK cells. Anti-p75 IL-2R mAb almost completely inhibited the induction of cytolytic activity in these T cells by brief exposure to IL-2, as estimated by anti-TCR/CD3 mAb-targeted cytotoxicity. While anti-p55 IL-2R mAb alone inhibited the response only modestly, maximal inhibition was achieved by combining both anti-p55 and anti-p75 IL-2R mAbs. These results indicate that the p75 IL-2R constitutively expressed on peripheral blood CD8+ T cells and TCR delta-1+ T cells is predominantly responsible for the direct activation of these cells by IL-2.
authors.affiliation: Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yagita
authors.forename: M
authors.initials: M
authors.lastname: Nakata
authors.forename: A
authors.initials: A
authors.lastname: Azuma
authors.forename: T
authors.initials: T
authors.lastname: Nitta
authors.forename: T
authors.initials: T
authors.lastname: Takeshita
authors.forename: K
authors.initials: K
authors.lastname: Sugamura
authors.forename: K
authors.initials: K
authors.lastname: Okumura
mesh_terms: D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology; D003602:Cytotoxicity, Immunologic; D006801:Humans; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation; D008970:Molecular Weight; D015375:Receptors, Interleukin-2 / Q000502:physiology* / Q000648:ultrastructure; D013601:T-Lymphocytes / Q000145:classification / Q000502:physiology*
chemical_list: D000911:Antibodies, Monoclonal; D007376:Interleukin-2; D015375:Receptors, Interleukin-2