(1----3)-alpha-D-glucan from an alkaline extract of Agrocybe cylindracea, and antitumor activity of its O-(carboxymethyl)ated derivatives.
The structure of an alkali-soluble D-glucan (AG-AL) from the fruit body of Agrocybe cylindracea was investigated by a combination of chemical and spectroscopic methods indicating that it was a linear (1----3)-alpha-D-glucan (molecular weight, approximately 560,000), [alpha]20D +195 degrees (c 0.5, M sodium hydroxide). Both water-soluble and gelatinous products obtained by O-(carboxymethyl)ation of AG-AL showed potent antitumor activity against the solid form of Sarcoma 180 in mice, although the native D-glucan had little effect on the tumor.
Kiho
T
T
Gifu Pharmaceutical University, Japan.
Yoshida
I
I
Nagai
K
K
Ukai
S
S
Hara
C
C
D000818:Animals; D001487:Basidiomycota / Q000032:analysis*; D002850:Chromatography, Gel; D005936:Glucans / Q000032:analysis* / Q000627:therapeutic use; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008970:Molecular Weight; D010084:Oxidation-Reduction; D010504:Periodic Acid / Q000378:metabolism; D012510:Sarcoma 180 / Q000188:drug therapy*; D012995:Solubility; D014407:Tumor Cells, Cultured / Q000187:drug effects
D005936:Glucans; D010504:Periodic Acid