[Effects of dauricine on the metabolism of arachidonic acid in rat pleural neutrophils].
The effects of dauricine (Dau), an isoquinoline alkaloid and anti-arrhythmic agent used in China recently, on the biosynthesis of metabolites of arachidonic acid in rat pleural neutrophils, comparing with dazoxiben, indomethacin and BW-755 c, were studied. The major products of metabolism by 5-lipoxygenase (5-LPO), measured by HPLC, were LTB4 and 5-HETE, whereas the major cyclooxygenase products measured by HPLC and RIA, were HHT and TXB2. The formation of all products by neutrophils was significantly depressed by Dau in a dose-dependent manner. The concentration of Dau required to obtain 50% inhibition (IC50) of formation of HHT, TXB2, LTB4 and 5-HETE was 25.3, 59.3, 31.8 and 59.5 mumol/L, respectively. These results indicate that the two major metabolic pathways of arachidonic acid in rat pleural neutrophils are inhibited by Dau.
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D000470:Alkaloids; D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism*; D044182:Benzylisoquinolines; D004305:Dose-Response Relationship, Drug; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis; D007546:Isoquinolines / Q000494:pharmacology*; D007975:Leukotriene B4 / Q000096:biosynthesis; D008297:Male; D009504:Neutrophils / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D044005:Tetrahydroisoquinolines; D013929:Thromboxane B2 / Q000096:biosynthesis
D000470:Alkaloids; D001095:Arachidonic Acids; D044182:Benzylisoquinolines; D005231:Fatty Acids, Unsaturated; D006893:Hydroxyeicosatetraenoic Acids; D007546:Isoquinolines; D044005:Tetrahydroisoquinolines; D007975:Leukotriene B4; C008033:12-hydroxy-5,8,10-heptadecatrienoic acid; D013929:Thromboxane B2; C035934:dauricine