Detection of the multidrug resistant phenotype in human tumours by monoclonal antibodies and the streptavidin-biotinylated phycoerythrin complex method.
The aim of this study was to find out whether the membrane glycoprotein P-170 can be detected in human tumours with both acquired and intrinsic resistance to chemotherapeutic agents using monoclonal antibodies (265/F4 and C219) and the streptavidin-biotinylated phycoerythrin complex method. Pretreated leukaemia cells and untreated lung and ovarian carcinomas were analysed. Two plasmacytomas and one leukaemia expressed high levels of P-glycoprotein, whereas two leukaemias showed moderate, and three leukaemias no expression of this protein. The intrinsic resistance was analysed with a panel of four human epidermoid lung cancer xenografts grown in nude mice. The expression of P-glycoprotein could be correlated with the degree of resistance. In addition, one out of five ovarian carcinomas revealed a high level of P-glycoprotein.
Volm
M
M
Department of Experimental Pathology, German Cancer Research Center, Heidelberg.
Efferth
T
T
Bak
M
M
Ho
A D
AD
Mattern
J
J
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000911:Antibodies, Monoclonal; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D001426:Bacterial Proteins; D001710:Biotin; D004351:Drug Resistance; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007938:Leukemia / Q000188:drug therapy / Q000378:metabolism*; D008175:Lung Neoplasms / Q000032:analysis*; D008562:Membrane Glycoproteins / Q000032:analysis*; D010051:Ovarian Neoplasms / Q000032:analysis*; D010799:Phycoerythrin; D019809:Streptavidin
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000911:Antibodies, Monoclonal; D000970:Antineoplastic Agents; D001426:Bacterial Proteins; D008562:Membrane Glycoproteins; D010799:Phycoerythrin; D001710:Biotin; D019809:Streptavidin