title: Expression of nerve growth factor receptors by human peripheral blood mononuclear cells.
abstract: In the rat, nerve growth factor (NGF) has been shown to affect immune reactivity by binding to cell surface receptors on a subpopulation of splenic mononuclear cells. This binding occurs in a specific and saturable fashion to what appear to be low-affinity (type II) NGF receptors (NGFR). Immunofluorescence studies here showed that NGFR are also present on a proportion of human peripheral blood mononuclear cells (PBMC). Equilibrium binding studies demonstrated that the binding of NGF to its receptors on PBMC occurs with a single equilibrium binding constant (mean) of 2.11 X 10(-9) M. The number of receptors per cell was determined to be approximately 6.94 X 10(3) receptors/cell. These results would suggest a role for NGF in the regulation of immune function in man, as well as in animals.
authors.affiliation: Department of Human Biological Chemistry, University of Texas Medical Branch, Galveston.
authors.forename: B
authors.initials: B
authors.lastname: Morgan
authors.forename: L W
authors.initials: LW
authors.lastname: Thorpe
authors.forename: D
authors.initials: D
authors.lastname: Marchetti
authors.forename: J R
authors.initials: JR
authors.lastname: Perez-Polo
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008297:Male; D009000:Monocytes / Q000378:metabolism*; D009414:Nerve Growth Factors / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000302:isolation & purification / Q000378:metabolism*; D017475:Receptors, Nerve Growth Factor; D012016:Reference Values; D012995:Solubility
chemical_list: D009414:Nerve Growth Factors; D011956:Receptors, Cell Surface; D017475:Receptors, Nerve Growth Factor