Influences of tromantadine and nonoxinol 9 on the stability of red cell membrane.
The antiviral compound tromantadine (ViruMerz) and the detergent nonoxinol 9 have been investigated in their effects on biophysical parameters of red cell membranes. Up to a maximum ratio of 1 mol per 800 mol of phospholipids tromantadine enhances the membrane phase transition/separation break at 16-20 degrees C measured by 1-anilinonaphthalene-sulfonate (ANS) fluorescence. It also increases order parameters obtained from spin labeling experiments with 5-doxyl stearic acid. Nonoxinol 9 interacts with the membrane at a maximum ratio of 1 mol per 40 mol of phospholipids determined by UV spectrophotometry. The substance decreases the intensity of the above phase transition/separation break and the order parameters of the spin label 5-doxyl stearic acid. These experiments indicate that tromantadine probably stabilizes the membrane whereas nonoxynol 9 exhibits opposite effects. Combination of both compounds equalizes the influences of the single substances on the above biophysical parameters of red cell membrane with predominance of the nonoxinol 9 effect.
Freisleben
H J
HJ
Gustav-Embden-Zentrum der Biologischen Chemie, Universität Frankfurt, Fed. Rep. of Germany.
Waltinger
G
G
Schatton
W
W
Zimmer
G
G
D000547:Amantadine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000817:Anilino Naphthalenesulfonates / Q000494:pharmacology; D002784:Cholesterol / Q000097:blood; D004578:Electron Spin Resonance Spectroscopy; D004910:Erythrocyte Membrane / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D017137:Nonoxynol; D010743:Phospholipids / Q000097:blood; D010783:Photometry; D011092:Polyethylene Glycols / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
D000817:Anilino Naphthalenesulfonates; D010743:Phospholipids; D017137:Nonoxynol; D011092:Polyethylene Glycols; D002784:Cholesterol; D000547:Amantadine; C004279:tromantadine