title: Multiple defects in Escherichia coli mutants lacking HU protein.
abstract: The HU protein isolated from Escherichia coli, composed of two partially homologous subunits, alpha and beta, shares some of the properties of eucaryotic histones and is a major constituent of the bacterial nucleoid. We report here the construction of double mutants totally lacking both subunits of HU protein. These mutants exhibited poor growth and a perturbation of cell division, resulting in the formation of anucleate cells. In the absence of HU, phage Mu was unable to grow, to lysogenize, or to carry out transposition.
authors.affiliation: Département de Biotechnologie, Institut Pasteur, Paris, France.
authors.forename: O
authors.initials: O
authors.lastname: Huisman
authors.forename: M
authors.initials: M
authors.lastname: Faelen
authors.forename: D
authors.initials: D
authors.lastname: Girard
authors.forename: A
authors.initials: A
authors.lastname: Jaffé
authors.forename: A
authors.initials: A
authors.lastname: Toussaint
authors.forename: J
authors.initials: J
authors.lastname: Rouvière-Yaniv
mesh_terms: D001426:Bacterial Proteins / Q000172:deficiency* / Q000235:genetics; D010583:Bacteriophage mu / Q000235:genetics / Q000254:growth & development; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins / Q000172:deficiency* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development; D005798:Genes, Bacterial; D008242:Lysogeny; D009154:Mutation; D010957:Plasmids; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; C040330:histone-like protein HU, bacteria