Effect of freezing and ultrasonication on the density of human lymphocyte beta 2-adrenoceptors.
In this study 21 pregnant volunteers were divided into three equal groups; blood samples were taken from each, and after isolation of the lymphocytes the cell suspensions were divided into two equal samples. The density of beta 2-adrenoceptor in intact lymphocytes was compared with the receptor density for lymphocytes stored 7 days or 19 days at -70 degrees C and with cells disrupted by ultrasonication. Storage by lymphocytes at -70 degrees C for 7 days to 19 days decreased the beta 2-adrenoceptor density by 20% and 37%, respectively; ultrasonication decreased the density by 65%.
Santala
M
M
Department of Obstetrics and Gynaecology, Kuopio University Central Hospital, Finland.
Castrén
O
O
Saarikoski
S
S
Penttilä
I
I
D000328:Adult; D002469:Cell Separation; D005260:Female; D005615:Freezing; D006801:Humans; D007958:Leukocyte Count; D008214:Lymphocytes / Q000378:metabolism*; D011247:Pregnancy; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D014465:Ultrasonics
D011943:Receptors, Adrenergic, beta