[Assay of NK cell activity and IL-2 and IFNr production in vitro in patients with epidemic hemorrhagic fever].
In order to study the immune function and pathogenesis of epidemic hemorrhagic fever (EHF), we assayed NK cell cytotoxicity against K562 targets in 95 patients with EHF and the production of IL-2 and IFNr in peripheral blood mononuclear cells (PBMC) after PHA stimulation In contrast to that in normal controls. NK cell activity elevated markedly during febrile phase and reached a highest level during shock and oliguric phase and then gradually decreased to normal. NK cell activity in patients with serious EHF elevated more markedly than that in patients with mild EHF. The production of IL-2 and IFNr in PBMC of patients with EHF after PHA stimulation was normal. The production of IL-2 had significantly positive correlation with NK, cell activity during oliguric, polyuric and convalescent phases, so was the production of IFNr with shock, oliguric, polyuric, and convalescent phases. These results suggests that patients with EHF have still normal cellular immune response. EHF virus may activate NK cells directly and NK cells may participate in the pathogenesis of EHF.
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D000293:Adolescent; D000328:Adult; D000368:Aged; D005260:Female; D006480:Hemorrhagic Fever with Renal Syndrome / Q000276:immunology*; D006801:Humans; D007111:Immunity, Cellular; D007371:Interferon-gamma / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000096:biosynthesis*; D007694:Killer Cells, Natural / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008875:Middle Aged
D007376:Interleukin-2; D007371:Interferon-gamma