An assay for dihydroorotase using high-performance liquid chromatography with radioactivity detection.
An assay for measuring dihydroorotase activity was devised. Radiolabeled substrate and product were separated by high-performance liquid chromatography using a reverse-phase column with ion-pairing, and the radioactivity was quantitated by flow detection.
Mehdi
S
S
Merrell Dow Research Institute, Cincinnati, Ohio 45215.
Wiseman
J S
JS
D000581:Amidohydrolases / Q000032:analysis*; D000818:Animals; D002250:Carbon Radioisotopes; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004080:Dihydroorotase / Q000032:analysis*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D011865:Radioisotope Dilution Technique; D051381:Rats
D002250:Carbon Radioisotopes; D000581:Amidohydrolases; D004080:Dihydroorotase