[Quantitative evaluation of the surface membrane receptors of leukemic cells to blood serum transcobalamin-II].
Serum protein transcobalamin II (TC-II) is responsible for transport of cobalamins into mammalian cells. A method of quantitative estimation of plasma membrane receptors of hemopoietic cells to TC-II cobalamin complex is suggested. Analysis of mouse leukemia L1210 cells includes the saturation of radiolabelled ligand-receptor complex with papain. The number of receptors and 57CoCNCbl content in one cell is determined by differentiated radioactivity count of solubilized protein complexes and of cytoplasm.
Oreshkin
A E
AE
Miasishcheva
N V
NV
D000818:Animals; D001692:Biological Transport; D002462:Cell Membrane / Q000032:analysis / Q000378:metabolism; D003037:Cobalt Radioisotopes; D007939:Leukemia L1210 / Q000097:blood*; D051379:Mice; D008811:Mice, Inbred DBA; D009368:Neoplasm Transplantation; D011869:Radioligand Assay / Q000379:methods; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000378:metabolism; D014155:Transcobalamins / Q000097:blood*
D003037:Cobalt Radioisotopes; D011956:Receptors, Cell Surface; D014155:Transcobalamins; C028176:transcobalamin receptor
Kolichestvennaia otsenka retseptorov poverkhnostnoĭ membrany leĭkoznykh kletok k transkobalaminu-II syvorotki krovi.