Virulence determinants of Salmonella typhimurium from animal sources.
Two hundred seventy-eight strains of Salmonella typhimurium isolated from 1973 to 1981 from animal sources in New York State were studied for possible virulence determinants and for a serotype-specific plasmid possibly linked with virulence. Of the strains, 98% possessed type-1 fimbriae. All strains possessed flagella and were motile. One hundred twenty-three strains (44%) treated with mitomycin C tested positive for the cholera-Escherichia coli heat labile family of toxins by a kinetics-based ELISA; when treated with mitomycin C and extracted with polymyxin B, 249 (90%) were positive in the kinetics-based ELISA. All strains were negative in the Biken test. A smooth cell wall was found in 99% of the strains. Sixty-one percent (169) of the strains had a 62-Md plasmid. Seventy-six (27%) of the strains had detectable plasmids ranging in size from 1 to 124 Md.
McDonough
P L
PL
Diagnostic Laboratory, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853.
Jacobson
R H
RH
Timoney
J F
JF
D000818:Animals; D001427:Bacterial Toxins / Q000032:analysis; D002473:Cell Wall / Q000648:ultrastructure; D004731:Endotoxins; D004768:Enterotoxins / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D010861:Fimbriae, Bacterial / Q000648:ultrastructure; D009518:New York; D010957:Plasmids; D012481:Salmonella Infections, Animal / Q000382:microbiology; D012486:Salmonella typhimurium / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity* / Q000648:ultrastructure; D014774:Virulence
D001427:Bacterial Toxins; D004731:Endotoxins; D004768:Enterotoxins; C027952:salmonella toxin