title: Vero cells co-infected with Chlamydia trachomatis and herpes simplex virus type 2: a scanning and transmission electron microscope study.
abstract: Vero (African Green Monkey Kidney) cells infected with Chlamydia trachomatis (serovar L2) (CT-L2) for 48 hr and superinfected with herpes simplex virus type 2 (HSV-2) were fixed 24 hr later and examined with the transmission electron microscope (TEM) and scanning electron microscope (SEM). Ultrastructural examination of the co-infected cells showed that, although many CT-L2 inclusions were present, most were empty of reticulate bodies or elementary bodies. However, large numbers of viruses were observed in the co-infected Vero cells and, in some instances, were seen inside the CT-L2 inclusions. SEM studies of the co-infected cells revealed a swelling and rounding up of the Vero cells typical of a CT-L2 or HSV-2 infection. The outer surface of the co-infected cells were covered with many long microvilli typical of HSV-2 infection with only a few surface protruberances similar to those found in CT-L2 infected cells.
authors.affiliation: Microbiology Research Division, Health and Welfare Canada, Ottawa, Ontario.
authors.forename: R D
authors.initials: RD
authors.lastname: Pontefract
authors.forename: C W
authors.initials: CW
authors.lastname: Ng
authors.forename: G
authors.initials: G
authors.lastname: Bergeron
mesh_terms: D000818:Animals; D002690:Chlamydia Infections / Q000473:pathology*; D002692:Chlamydia trachomatis / Q000648:ultrastructure*; D006561:Herpes Simplex / Q000473:pathology*; D008855:Microscopy, Electron, Scanning; D018139:Simplexvirus / Q000648:ultrastructure*; D014709:Vero Cells