[Rapid diagnosis of cytomegalovirus in immunocompromised patients using monoclonal antibodies that detect early viral antigens].
A technique was applied to detect early fluorescent antigens (DEFA) of cytomegalovirus (CMV) using the E13 monoclonal antibodies in 52 immuno-compromised patients hospitalized in the Nephrology Institute of Havana. Of the 75 urine or blood (buffy coat) samples taken, 15 were found positive to CMV. Using classical diploid human fibroblast isolation technique, 12 CMV strains were isolation of previously detected positive samples by DEFA. In addition, CMV was isolated from one sample reported to be negative by DEFA. A coincidence of 80% was found between both techniques. With the ELISA test, all the sample studied have IgG antibodies to CMV.
Alvarez
M
M
Instituto Medicina Tropical Pedro Kouri, Departamento de Virología, Ciudad de La Habana, Cuba.
Marrero
M
M
Soler
M
M
Puig
L
L
Moreno
D
D
Reyes
L
L
Castillo
A
A
D000293:Adolescent; D000328:Adult; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis*; D003587:Cytomegalovirus / Q000276:immunology* / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D005260:Female; D006801:Humans; D007108:Immune Tolerance; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D006435:Renal Dialysis; D012698:Serologic Tests
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral
Diagnostico Rápido de citomegalovirus (CMV) en pacientes inmunocomprometidos mediante anticuerpos monoclonales que reconocen proteínas precoces virales.