Retinoic acid regulation of the expression of retinoic acid receptors in wild-type and mutant embryonal carcinoma cells.
The embryonal carcinoma cell line P19 undergoes terminal differentiation upon induction by retinoic acid (RA). The effects of RA on the retinoic acid receptor (RAR) mRNA level were investigated in P19 cells and an RA non-responsive mutant RAC65. RA induced a rapid accumulation of RAR alpha within 2 h and this was followed by an increase in the RAR beta mRNA. RAC65 cells differed from P19 cells in that the accumulation of RAR alpha mRNA was delayed and that an aberrant pattern of RAR alpha transcript was observed.
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Banting and Best Department of Medical Research, C.H. Best Institute, University of Toronto, Ontario, Canada.
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D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D054278:Embryonal Carcinoma Stem Cells; D005786:Gene Expression Regulation / Q000187:drug effects; D015151:Immunoblotting; D015336:Molecular Probe Techniques; D009154:Mutation; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D018168:Receptors, Retinoic Acid; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D002352:Carrier Proteins; D012333:RNA, Messenger; D018168:Receptors, Retinoic Acid; D014212:Tretinoin