Isolation and characterization of the phosphoglucose isomerase gene from Escherichia coli.
The nucleotide sequence of the gene encoding the glycolytic enzyme phosphoglucose isomerase (PGI) from Escherichia coli is presented. The gene encodes a polypeptide of 549 amino acids. The transcriptional start point of the gene was determined and found to lie within a consensus promoter region. The amino acid sequence derived from the E. coli PGI gene can be aligned without insertions or deletions to the predicted amino acid sequence of a nuclear-encoded chloroplast isozyme of PGI from a higher plant, and the two sequences have a similarity of 87.6%. The amino acid sequence similarity between E. coli and that predicted from cDNA sequences for mouse and pig PGI is approximately 65%.
Froman
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BE
Department of Genetics, University of California, Davis 95616.
Tait
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Gottlieb
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LD
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA; D015342:DNA Probes; D004269:DNA, Bacterial; D004591:Electrophoresis, Polyacrylamide Gel; D004720:Endonucleases; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005956:Glucose-6-Phosphate Isomerase / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D010944:Plants / Q000235:genetics; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013045:Species Specificity
D015342:DNA Probes; D004269:DNA, Bacterial; D012333:RNA, Messenger; D004247:DNA; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases; D005956:Glucose-6-Phosphate Isomerase