[Study of polymorphism in human rRNA gene clusters].
Human ribosomal DNA (rDNA) probe specific for the 3' end of the 28S rRNA gene was used for detecting standard restriction fragments' length polymorphism (RFLPs) in the non-transcribed spacer. The conditions for hybridization of rDNA probe which eliminate cross hybridization of parts of 28S rRNA gene were developed. A test for detecting incompletely restricted DNA was also developed which may be used in experiments for detecting new RFLPs. It was found that a set of standard RFLPs was identical in various human tissues for one individual. Frequency of standard RFLPs in the non-transcribed spacer of human rRNA gene clusters was calculated.
Skriabin
B V
BV
Gindilis
V M
VM
Kupriianova
N S
NS
Timofeeva
M Ia
MIa
D001345:Autoradiography; D001483:Base Sequence; D003001:Cloning, Molecular; D004275:DNA, Ribosomal / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length; D012335:RNA, Ribosomal / Q000235:genetics*; D012339:RNA, Ribosomal, 28S / Q000235:genetics*
D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; D012339:RNA, Ribosomal, 28S
Issledovanie polimorfizma v predelakh klastera genov rRNK cheloveka.