title: Isolation and characterisation of transposon-induced mutants of Erwinia carotovora subsp. atroseptica exhibiting reduced virulence.
abstract: The blackleg pathogen Erwinia carotovora subsp. atroseptica (Eca) causes an economically important disease of potatoes. We selected a genetically amenable Eca strain for the genetic analysis of virulence. Tn5 mutagenesis was used to generate nine mutants which exhibited reduced virulence (Rvi-) of strain SCRI1043. Following physiological characterisation, mutants were divided into three classes: (1) auxotrophs; (2) extracellular enzyme mutants; and (3) a growth rate mutant. The isolation of these Rvi- mutants has allowed us to consider some factors that affect Eca virulence.
authors.affiliation: Department of Biological Sciences, University of Warwick, Coventry, England.
authors.forename: J C
authors.initials: JC
authors.lastname: Hinton
authors.forename: J M
authors.initials: JM
authors.lastname: Sidebotham
authors.forename: L J
authors.initials: LJ
authors.lastname: Hyman
authors.forename: M C
authors.initials: MC
authors.lastname: Pérombelon
authors.forename: G P
authors.initials: GP
authors.lastname: Salmond
mesh_terms: D015169:Colony Count, Microbial; D003227:Conjugation, Genetic; D003470:Culture Media; D004251:DNA Transposable Elements; D004885:Erwinia / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism / Q000472:pathogenicity; D005838:Genotype; D009154:Mutation; D010641:Phenotype; D010935:Plant Diseases; D010957:Plasmids; D011198:Solanum tuberosum / Q000382:microbiology; D014161:Transduction, Genetic; D014774:Virulence
chemical_list: D003470:Culture Media; D004251:DNA Transposable Elements