Expression of cellular retinoic acid binding protein II (chick-CRABP II) in the chick embryo.
We previously demonstrated the presence of cellular retinoic acid binding protein II, chick-CRABP II, in chick embryos. In the present study, we investigated the distribution of chick-CRABP II in 14-day chick embryos by means of immunoblot analysis. Chick-CRABP II was expressed in skin, muscle, bone with tendon of the embryos, but not expressed in the nervous system. In adult chick tissues, chick-CRABP II was not detected on immunoblotting; Chick-CRABP II in adults amounts to less than 10 ng/mg soluble protein. These observations suggest that chick- CRABP II is an embryonic protein involved in the development of specific tissues, such as bone, muscle and skin.
Kitamoto
T
T
National Institute of Neuroscience, NCNP, Japan.
Momoi
M
M
Momoi
T
T
D000818:Animals; D015153:Blotting, Western; D001842:Bone and Bones / Q000032:analysis; D002352:Carrier Proteins / Q000032:analysis*; D002642:Chick Embryo; D004591:Electrophoresis, Polyacrylamide Gel; D009132:Muscles / Q000032:analysis; D018168:Receptors, Retinoic Acid; D012867:Skin / Q000032:analysis
D002352:Carrier Proteins; D018168:Receptors, Retinoic Acid