title: Human neutrophils are not severely injured in conditions mimicking social drinking.
abstract: In an attempt to assess the effect of alcohol per se on human polymorphonuclear neutrophils (PMN), irrespective of other physiopathological parameters, we examined neutrophil function in healthy volunteers who had taken a single large dose of whisky. Before and at different times after ingestion, several PMN properties were simultaneously tested including random migration, in vitro chemotaxis, adherence, aggregation, cytochrome C reduction, phagocytosis, bacterial killing, intracellular cAMP and cGMP contents, myeloperoxidase, and neutrophil alkaline phosphatase scores. Only phagocytosis of Staphylococcus aureus was significantly depressed after alcohol ingestion. Adherence was inhibited only in some individuals when their respective blood alcohol levels were the highest. Both alterations were moderate and reversible. These data point out the limited effect of occasional alcohol consumption on the different facets of neutrophil behavior. These findings suggest that factors other than alcohol itself could be concerned in the marked PMN dysfunction well established in chronic alcoholism.
authors.affiliation: Central Laboratory of Haematology, CHU Rangueil, Toulouse, France.
authors.forename: J X
authors.initials: JX
authors.lastname: Corberand
authors.forename: P F
authors.initials: PF
authors.lastname: Laharrague
authors.forename: G
authors.initials: G
authors.lastname: Fillola
mesh_terms: D000428:Alcohol Drinking / Q000502:physiology*; D001770:Blood Bactericidal Activity / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D002449:Cell Aggregation / Q000187:drug effects; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D003574:Cytochrome c Group / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004734:Energy Metabolism / Q000187:drug effects; D000431:Ethanol / Q000493:pharmacokinetics / Q000494:pharmacology*; D006801:Humans; D007108:Immune Tolerance / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects*; D010587:Phagocytosis / Q000187:drug effects*
chemical_list: D003574:Cytochrome c Group; D000431:Ethanol