Granulocyte-macrophage colony-stimulating factor (GM-CSF) and opsonization synergistically enhance leukotriene B4 (LTB4) synthesis induced by phagocytosis in human neutrophils.
Normal human blood neutrophils were studied for their capacity to synthesize leukotriene B4 (LTB4) and its omega-oxidized metabolites after phagocytosis of zymosan. Phagocytosis of serum-opsonized particles led to a higher release of LTs than did unopsonized zymosan. The most striking effect of phagocytosis was observed when neutrophils were primed with granulocyte-macrophage colony-stimulating factor (GM-CSF): opsonization and GM-CSF synergistically increased LTB4 synthesis by neutrophils.
Poubelle
P E
PE
Unité de Recherche Inflammation et Immunologie-Rhumatologie, Centre de Recherche du CHUL, Qué, Canada.
Bourgoin
S
S
Naccache
P H
PH
Borgeat
P
P
D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003115:Colony-Stimulating Factors / Q000494:pharmacology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007975:Leukotriene B4 / Q000096:biosynthesis*; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D009895:Opsonin Proteins / Q000032:analysis; D010587:Phagocytosis / Q000187:drug effects; D015054:Zymosan / Q000494:pharmacology
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D009895:Opsonin Proteins; D007975:Leukotriene B4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D015054:Zymosan; D001094:Arachidonate 5-Lipoxygenase