Human CD2 3'-flanking sequences confer high-level, T cell-specific, position-independent gene expression in transgenic mice.
We have localized a set of T cell-specific DNAase I hypersensitive sites in the 3'-flanking region of the human CD2 gene. A 5.5 kb BamHI-XbaI fragment containing these DNAase I hypersensitive sites conferred efficient, copy number-dependent, T cell-specific expression of a linked human CD2 minigene, independent of the position of integration in the transgenic mouse genome. When linked to the mouse Thy-1.1 gene or the human beta-globin gene, this fragment conferred the same T cell-specific expression, independent of its orientation. These results suggest that this flanking region is both necessary and sufficient for full tissue-specific activation of homologous and heterologous genes in transgenic mice.
Greaves
D R
DR
Laboratory of Gene Structure and Expression, National Institute for Medical Research, London, England.
Wilson
F D
FD
Lang
G
G
Kioussis
D
D
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis / Q000235:genetics*; D000954:Antigens, Surface / Q000235:genetics; D001483:Base Sequence; D018801:CD2 Antigens; D002874:Chromosome Mapping; D003850:Deoxyribonuclease I / Q000378:metabolism; D005786:Gene Expression Regulation; D005914:Globins / Q000235:genetics; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics*; D011971:Receptors, Immunologic / Q000032:analysis / Q000235:genetics*; D013601:T-Lymphocytes / Q000276:immunology*; D018800:Thy-1 Antigens
D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D018801:CD2 Antigens; D011971:Receptors, Immunologic; D018800:Thy-1 Antigens; D005914:Globins; D003850:Deoxyribonuclease I