Molecular target size of an L-glutamate receptor from the cockroach central nervous system.
L-Glutamate binding to cockroach central nervous system (CNS) membranes is unaffected by freeze-drying and subsequent rehydration. The dissociation constant is similarly unaltered by irradiation at a dose of 3 Mrad which affects only the number of biologically active binding sites. A molecular weight estimate for this insect CNS L-glutamate receptor was performed using irradiation inactivation analysis. By this method a molecular target size of approximately 77.8 kDa was determined. This figure is in agreement with the molecular weight found by other authors for L-glutamate binding sites in the rat brain.
Sepúlveda
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MI
AFRC Unit of Insect Neurophysiology and Pharmacology, Department of Zoology, University of Cambridge, U.K.
Sattelle
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DB
D000818:Animals; D003058:Cockroaches / Q000378:metabolism*; D005612:Freeze Drying; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D008297:Male; D008970:Molecular Weight; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter / Q000032:analysis* / Q000528:radiation effects
D005971:Glutamates; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter; D018698:Glutamic Acid