BK virus (BKV) detection in urine specimens of immunocompromised patients: comparison between the DNA-DNA hybridization assay, the immunofluorescence test and the dot enzyme immunoassay.
Three methods used for the detection of BK virus in urine specimens, the indirect immunofluorescence test, the dot enzyme immunoassay and the DNA-DNA hybridization assay, were compared by testing specimens from 49 immunocompromised patients. All three assays were effective in detecting BK virus. The technical advantage of each of them was discussed. The immunofluorescence test was found to be the simplest one to perform; the DNA-DNA hybridization assay displayed exquisite sensitivity; and the easy reading of the dot enzyme immunoassay did not require the specialized training inherent to immunofluorescence assays. The dot enzyme immunoassay might therefore be the most practical method for screening urine specimens of immunocompromised patients, especially when the sediment is poor in cells. Conversely, the indirect immunofluorescence test might be the method of choice for checking patients with haemorragic cystitis whose urine samples usually contain large amounts of cells.
Marrero
M
M
Institut Pedro Khouri, Havana.
Garbarg-Chenon
A
A
de Saint-Maur
G
G
Fanen
P
P
Rousseau
E
E
Alvarez
M
M
Nicolas
J C
JC
Bricout
F
F
D001739:BK Virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D004279:DNA, Viral / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007108:Immune Tolerance; D007124:Immunoenzyme Techniques; D009693:Nucleic Acid Hybridization; D011120:Polyomavirus / Q000302:isolation & purification*; D014556:Urine / Q000382:microbiology*
D004279:DNA, Viral