A spectrophotometric method for the assay of pyrimidine 5'-nucleotidase in human erythrocytes.
A new spectrophotometric assay for human erythrocyte pyrimidine 5'-nucleotidase using a continuous spectrophotometric method is described. The activity is detected by measuring uracil, which is produced by a coupled assay including cytidine deaminase and uridine nucleosidase. The results obtained with the continuous spectrophotometric method have been confirmed by high pressure liquid chromatography (HPLC) analysis. This procedure, not based upon inorganic phosphate formation and avoiding the use of radioactive material, offers significant advantages with respect to commonly adopted assays.
Amici
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Istituto di Biochimica, Facolta' di Medicina e Chirurgia, Universita' di Ancona, Italy.
Natalini
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P
Ruggieri
S
S
Vita
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A
Magni
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G
D015720:5'-Nucleotidase / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D003564:Cytidine Deaminase; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015636:Magnesium Chloride / Q000494:pharmacology; D009699:N-Glycosyl Hydrolases; D013053:Spectrophotometry / Q000379:methods*
D015636:Magnesium Chloride; C023277:uridine nucleosidase; D015720:5'-Nucleotidase; D009699:N-Glycosyl Hydrolases; D003564:Cytidine Deaminase