Mapping replication units in animal cells.
A general approach for assaying the in vivo direction of replication for any DNA segment has been developed. This technique allows the scanning of genomic regions to detect bidirectional tail-to-tail replication, indicating the presence of a functional origin. By this criterion we identified the approximate positions of two origin sites downstream of the Chinese hamster DHFR gene. Further mapping revealed areas of head-to-head replication, signifying locations of replication termination and thus defining the landmarks of a complete animal cell replicon. Genetic proof for the existence of the DHFR origin was obtained by showing that this region serves as a bidirectional DNA synthesis initiation point following its integration into other sites in the genome by transfection. To show the general applicability of this methodology, we studied the APRT domain. Replication mapping together with the use of deletion mutants allowed the identification of an origin at a far-upstream locus.
Handeli
S
S
Department of Cellular Biochemistry Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Klar
A
A
Meuth
M
M
Cedar
H
H
D000228:Adenine Phosphoribosyltransferase / Q000235:genetics*; D000818:Animals; D001665:Binding Sites; D002874:Chromosome Mapping; D002875:Chromosomes / Q000648:ultrastructure*; D003001:Cloning, Molecular; D006224:Cricetinae; D015342:DNA Probes; D004261:DNA Replication; D005786:Gene Expression Regulation; D010430:Pentosyltransferases / Q000235:genetics*; D015183:Restriction Mapping; D013539:Simian virus 40 / Q000235:genetics; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*; D014162:Transfection
D015342:DNA Probes; D013762:Tetrahydrofolate Dehydrogenase; D010430:Pentosyltransferases; D000228:Adenine Phosphoribosyltransferase