Definition of CD 11a, b, c, and CD 18 glycoproteins on chemotactically deficient granulocyte membranes in patients affected by myeloid disorders.
We studied neutrophil chemotaxis and surface membrane glycoproteins in 12 patients suffering from myeloid disorders with abnormal karyotype using in vitro techniques in all 12. Chromosome studies were also carried out virtually simultaneously. We chose to study only patients showing a deficit of chemotaxis (p less than 0.001). Ten of these patients revealed a clonal chromosome abnormality in most of the leukemic cells. The monoclonal antibody technique using MoAb 60.1, 60.3, 60.5, OKM1, LFA-1 and 9E8 demonstrated normal leukocyte membrane glycoproteins CD 11a, b, c and CD 18 and therefore excludes that an abnormality of these is involved in the pathogenesis of altered leukocyte chemotaxis. No correlation between chemotactic deficit and any specific clonal chromosome abnormality was found.
Ricevuti
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G
Department of Internal Medicine and Therapeutics, University of Pavia, Italy.
Mazzone
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Notario
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D000754:Anemia, Refractory, with Excess of Blasts / Q000097:blood* / Q000276:immunology; D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000097:blood*; D001856:Bone Marrow Examination; D018821:CD18 Antigens; D002465:Cell Movement; D002634:Chemotaxis, Leukocyte; D002869:Chromosome Aberrations; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000097:blood*; D006098:Granulocytes / Q000276:immunology; D006801:Humans; D016167:Integrin alphaXbeta2; D007621:Karyotyping; D015470:Leukemia, Myeloid, Acute / Q000097:blood* / Q000276:immunology; D016169:Lymphocyte Function-Associated Antigen-1; D016177:Macrophage-1 Antigen; D008562:Membrane Glycoproteins / Q000097:blood*; D008565:Membrane Proteins / Q000097:blood*; D009190:Myelodysplastic Syndromes / Q000097:blood* / Q000276:immunology; D009504:Neutrophils / Q000276:immunology
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D018821:CD18 Antigens; D006023:Glycoproteins; D016167:Integrin alphaXbeta2; D016169:Lymphocyte Function-Associated Antigen-1; D016177:Macrophage-1 Antigen; D008562:Membrane Glycoproteins; D008565:Membrane Proteins