title: Phosphorylation of the carotenoid droplet protein p57 by the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase and the effect of fluoride.
abstract: We have previously shown that the dispersion and aggregation of carotenoid droplets in goldfish xanthophores are regulated, respectively, by phosphorylation and dephosphorylation of a carotenoid droplet protein p57. There is a basal level of p57 phosphorylation of p57 in unstimulated cells, which is greatly stimulated by adrenocorticotropic hormone (ACTH) or cyclic adenosine monophosphate (cAMP) acting via cAMP-dependent protein kinase. We have also observed that, in permeabilized xanthophores, pigment dispersion can be induced when cAMP is replaced by fluoride. Since p57 has multiple phosphorylation sites, there is the question of whether all p57 phosphorylation is by cAMP-dependent protein kinase or whether phosphorylation by cAMP-independent protein kinase coupled with inhibition of phosphatase activity by fluoride can replace cAMP-dependent protein kinase and that the ability of fluoride to replace cAMP for pigment dispersion in permeabilized cells is probably due to activation of adenylcyclase. We also show that ACTH causes an approximately threefold increase in the level of cAMP in these cells.
authors.affiliation: Department of Chemistry and Biological Sciences, Wayne State University, Detroit, MI 48202.
authors.forename: C F
authors.initials: CF
authors.lastname: Yang
authors.forename: Z C
authors.initials: ZC
authors.lastname: Zeng
authors.forename: S C
authors.initials: SC
authors.lastname: Chou
authors.forename: F X
authors.initials: FX
authors.lastname: Yu
authors.forename: J D
authors.initials: JD
authors.lastname: Taylor
authors.forename: T T
authors.initials: TT
authors.lastname: Tchen
mesh_terms: D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000818:Animals; D002856:Chromatophores / Q000187:drug effects / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism / Q000494:pharmacology; D006054:Goldfish; D007700:Kinetics; D010766:Phosphorylation; D011494:Protein Kinases / Q000302:isolation & purification / Q000378:metabolism*; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism*; D012969:Sodium Fluoride / Q000494:pharmacology*
chemical_list: D011506:Proteins; D015124:8-Bromo Cyclic Adenosine Monophosphate; D012969:Sodium Fluoride; D000324:Adrenocorticotropic Hormone; D000242:Cyclic AMP; D011494:Protein Kinases