Decreased binding of vasoactive intestinal peptide to intestinal epithelial cells from hypothyroid rats.
The binding of vasoactive intestinal peptide (VIP) and stimulation of adenylate cyclase by VIP were studied in intestinal epithelial cells during hypothyroidism. Experimental hypothyroidism was induced in rats by the administration of KC10(4). The binding capacity, but not the affinity, of VIP receptors decreased in the hypothyroid rats. Besides, the stimulation of cyclic AMP production by VIP was also diminished in cells from hypothyroid rats. These observations indicate a decrease of the responsiveness of intestinal epithelial cells to VIP in the hypothyroid status, suggesting a role of the peptide in the pathophysiologic mechanism of intestinal manifestations during hypothyroidism.
Molinero
P
P
Department of Medical Biochemistry and Molecular Biology, School of Medicine, University of Sevilla, Spain.
Calvo
J R
JR
Jimenez
J
J
Goberna
R
R
Guerrero
J M
JM
D000818:Animals; D000242:Cyclic AMP / Q000096:biosynthesis; D004848:Epithelium / Q000378:metabolism; D007037:Hypothyroidism / Q000139:chemically induced / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000187:drug effects; D007700:Kinetics; D008297:Male; D010472:Perchlorates; D011188:Potassium; D017680:Potassium Compounds; D051381:Rats; D011919:Rats, Inbred Strains; D011964:Receptors, Gastrointestinal Hormone / Q000378:metabolism; D018005:Receptors, Vasoactive Intestinal Peptide; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism* / Q000494:pharmacology
D010472:Perchlorates; D017680:Potassium Compounds; D011964:Receptors, Gastrointestinal Hormone; D018005:Receptors, Vasoactive Intestinal Peptide; D014660:Vasoactive Intestinal Peptide; C009006:potassium perchlorate; D000242:Cyclic AMP; D011188:Potassium