Effects of E. coli 0111.B4 lipopolysaccharide on spin-labelled murine macrophage and hepatocyte membranes.
Macrophages and hepatocytes from normal and BCG-primed mice have been spin-labelled in their membranes with 5- and 16-doxyl stearic acid. Incubation of spin-labelled cells from BCG-primed animals with lipopolysaccharide from E. coli 0111.B4 produced a detectable and transient disturbance in the cell membranes as reflected by an increase in the order parameter measured from the electron spin resonance spectra of 5-doxyl-stearate. This membrane disturbance was maximal at 3-4 hours of incubation and was only detected with cells from mice primed with BCG. Spectra obtained from the 16-doxyl-stearate-labelled cells showed no change in order parameter on incubation with lipopolysaccharide.
Jackson
S K
SK
Dept Medical Microbiology, University of Wales College of Medicine, Cardiff.
James
P E
PE
Rowlands
C C
CC
Evans
J C
JC
D000818:Animals; D001500:BCG Vaccine / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects; D004578:Electron Spin Resonance Spectroscopy; D004926:Escherichia coli; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000633:toxicity*; D008099:Liver / Q000187:drug effects* / Q000648:ultrastructure; D008264:Macrophages / Q000187:drug effects* / Q000648:ultrastructure; D051379:Mice; D008815:Mice, Inbred Strains; D013113:Spin Labels
D001500:BCG Vaccine; D008070:Lipopolysaccharides; D013113:Spin Labels