Treatment of mouse thymus cells with phosphatidylinositol-specific phospholipase C preferentially abrogates their reactivity with autoantibodies to the Thy-1 antigen.
Thy-1 molecules have been shown to become anchored to the membrane of thymocytes and of T-cells via a phosphatidylinositol link. In the present study the intensity of immunofluorescent staining of mouse thymus cells by monoclonal xenoantibodies and alloantibodies specific for the Thy-1.2 determinant was significantly reduced following exposure of the cells to phosphatidylinositol-specific phospholipase C (PI-PLC). The majority of the PI-PLC-treated thymus cells retained, however, some reactivity with Thy-1.2 antibodies. In contrast, the immunofluorescent staining of thymus cells with monoclonal autoantibodies to Thy-1 determinants (20-10-5 and C16-31) was completely abolished by PI-PLC treatment. These results suggest that whereas monoclonal autoantibodies to Thy-1 react preferentially with PI-PLC-sensitive Thy-1 molecules, monoclonal antibodies to the Thy-1.2 specificity react with all cell surface Thy-1 molecules, regardless of their sensitivity to PI-PLC treatment.
Schlesinger
M
M
Hubert H. Humphrey Center for Experimental Medicine and Cancer Research, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Kincade
P W
PW
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology*; D001323:Autoantibodies / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D054801:Phosphoinositide Phospholipase C; D010727:Phosphoric Diester Hydrolases / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D018800:Thy-1 Antigens
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D001323:Autoantibodies; D018800:Thy-1 Antigens; D010727:Phosphoric Diester Hydrolases; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase