Homologous and heterologous regulation of the helodermin/vasoactive-intestinal-peptide response in the murine radiation leukemia-virus-induced lymphoma cell line BL/VL3.
1. Functional vasoactive intestinal peptide (VIP)/helodermin receptors and beta 2-adrenoceptors coexist in membranes from a cultured cloned BL/VL3 cell line of murine T-cell lymphoma induced by a radiation leukemia virus (see preceding paper in this journal). 2. Short-term (5-30 min) exposures of BL/VL3 cells to VIP or isoproterenol induced both homologous and heterologous desensitization. The potency of VIP and isoproterenol to desensitize was similar to their potency to occupy receptors and activate adenylate cyclase. 3. Long-term (16-h) exposure of BL/VL3 cells to VIP induced homologous down regulation only, whereas isoproterenol induced both homologous and heterologous down regulation. The potency of VIP, peptide histidine isoleucinamide, helodermin, helospectin, and [D-Phe2]VIP on the one hand, and of isoproterenol on the other hand, to decrease homologous responses was comparable to their potency for receptor occupancy and adenylate cyclase activation.
Abello
J
J
Department of Biochemistry and Nutrition, Medical School, Université Libre de Bruxelles.
Damien
C
C
Robberecht
P
P
Hooghe
R
R
Vandermeers
A
A
Vandermeers-Piret
M C
MC
Christophe
J
J
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D004361:Drug Tolerance; D004789:Enzyme Activation / Q000187:drug effects; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology; D036341:Intercellular Signaling Peptides and Proteins; D007545:Isoproterenol / Q000008:administration & dosage / Q000494:pharmacology; D007953:Leukemia, Radiation-Induced; D008223:Lymphoma / Q000378:metabolism*; D051379:Mice; D010451:Peptide PHI / Q000494:pharmacology; D010455:Peptides / Q000008:administration & dosage / Q000494:pharmacology*; D011943:Receptors, Adrenergic, beta / Q000378:metabolism; D012190:Retroviridae; D012969:Sodium Fluoride / Q000494:pharmacology; D013601:T-Lymphocytes; D014407:Tumor Cells, Cultured; D014660:Vasoactive Intestinal Peptide / Q000008:administration & dosage / Q000494:pharmacology*
D036341:Intercellular Signaling Peptides and Proteins; D010451:Peptide PHI; D010455:Peptides; D011943:Receptors, Adrenergic, beta; D006165:Guanylyl Imidodiphosphate; D014660:Vasoactive Intestinal Peptide; C040442:heliodermin; D012969:Sodium Fluoride; C043046:helospectin I; C043047:helospectin II; D000262:Adenylyl Cyclases; D007545:Isoproterenol