The use of a DNA probe for the differentiation of rodent malaria strains and species.
A clone, PCsv4, derived from a partial genomic library of the rodent malaria Plasmodium chabaudi chabaudi AS strain, contains an insert which when used as a probe at low stringency in Southern blotting of genomic DNA from a variety of strains, subspecies and species of rodent malaria parasites, results in a pattern of hybridisation which is specific to each of the DNA samples used, thus providing an accurate method to define a rodent malaria line. The insert only hybridises to DNA derived from malaria parasites of rodent species. The insert also hybridises to a small number of RNA transcripts.
Viriyakosol
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Division of Parasitology, National Institute for Medical Research, Mill Hill, London, U.K.
Snounou
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Brown
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D000818:Animals; D015139:Blotting, Southern; D015342:DNA Probes / Q000302:isolation & purification* / Q000648:ultrastructure; D004587:Electrophoresis, Agar Gel; D008288:Malaria / Q000469:parasitology / Q000662:veterinary; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D009696:Nucleic Acids / Q000302:isolation & purification; D010961:Plasmodium / Q000145:classification* / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D012376:Rodent Diseases / Q000382:microbiology; D013045:Species Specificity
D015342:DNA Probes; D009696:Nucleic Acids