Stimulation of FA and phosphatase-1 activities by insulin in 3T3-L1 cells.
The phosphatase-1 activator FA and phosphatase-1 were assayed in 3T3-L1 cells exposed to insulin. The cytosolic FA activity was transiently stimulated (7-8-fold) 1 and 2 min after exposure to 10(-8) M insulin and returned to control values within 5-10 min. Cytosolic phosphatase-1 (assayed after trypsin treatment) was activated (120-140% of controls) between 2 and 5 min and returned to control values within 10 min. Insulin effects were dose-dependent, with maximum stimulation of both activities at 10(-8) M insulin. The possibility that FA and other kinases mediate phosphatase activation by insulin is discussed.
Villa-Moruzzi
E
E
Istituto di Patologia Generale, Università di Pisa, Italy.
D000818:Animals; D002478:Cells, Cultured; D003600:Cytosol / Q000201:enzymology; D004305:Dose-Response Relationship, Drug; D038362:Glycogen Synthase Kinase 3; D007328:Insulin / Q000494:pharmacology*; D007700:Kinetics; D051379:Mice; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism*; D054645:Protein Phosphatase 1
D007328:Insulin; D011494:Protein Kinases; D038362:Glycogen Synthase Kinase 3; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1