title: Effects of transglutaminase substrates and inhibitors on the motility of demembranated reactivated spermatozoa.
abstract: The effects of transglutaminase (TGase) substrates putrescine, dansylcadaverine, spermine, etc., and the TGase inhibitor cystamine were tested on the motility of demembranated mammalian spermatozoa. These products blocked within a few seconds the motility of demembranated reactivated spermatozoa at concentrations ranging from 0.25 to 5 mM. These minimal inhibitory concentrations could be decreased 5-150-fold when TGase substrates and inhibitor were incubated with demembranated spermatozoa for 15 min prior to the addition of Mg.ATP. The inhibition was reversed by higher concentrations of Mg.ATP but none of these TGase substrates or inhibitor could inhibit bull sperm dynein ATPase. TGase activities, as measured by the incorporation of 3H-putrescine into TCA-precipitable proteins, were present in both sperm Triton-soluble and -insoluble fractions. On the other hand, amine acceptor protein substrates for the TGase-catalyzed reaction were present only in the insoluble fraction. The Triton-soluble TGase was similar to the known "tissue" TGases; the Triton-insoluble TGase activity was calcium independent. The same TGase substrates and inhibitor that blocked the motility of reactivated spermatozoa also blocked TGase activities. Linear relationships were observed between the concentrations of these substances required to block sperm motility and those to block TGase activities. These data suggest the involvement of a TGase activity in sperm motility.
authors.affiliation: Urology Research Laboratory, Royal Victoria Hospital, Montreal, Quebec, Canada.
authors.forename: E
authors.initials: E
authors.lastname: de Lamirande
authors.forename: C
authors.initials: C
authors.lastname: Gagnon
mesh_terms: D000818:Animals; D002103:Cadaverine / Q000031:analogs & derivatives / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D006224:Cricetinae; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004492:Edetic Acid / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D011073:Polyamines / Q000008:administration & dosage / Q000494:pharmacology*; D011700:Putrescine / Q000494:pharmacology; D011817:Rabbits; D051381:Rats; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects; D013096:Spermine / Q000494:pharmacology; D011503:Transglutaminases / Q000032:analysis / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D011073:Polyamines; D013096:Spermine; D004492:Edetic Acid; D011503:Transglutaminases; C008542:monodansylcadaverine; D002103:Cadaverine; D002118:Calcium; D011700:Putrescine