Antibodies to beta 2-microglobulin, the light-chain component of class I histocompatibility molecules, provide an activation signal to human lymphocytes.
Antibodies to beta 2-microglobulin (beta 2m), the light-chain component of class I histocompatibility antigens, provide a strong co-stimulatory signal to human lymphocytes in the presence of phorbol ester. This activation signal requires a high concentration of antibody, whereas the effect on responses to a mitogenic lectin is exclusively inhibitory over a broad dose range. A rabbit polyclonal and a mouse monoclonal antibody to beta 2m were similarly co-stimulatory. Antibody to beta 2m was co-stimulatory with phorbol ester, but not calcium ionophore, suggesting that class I antigens play a role in the initiation of the 'first' signal of a recently described two-signal model of lymphocyte activation.
Hashimoto
S
S
Department of Medicine, Louisiana State University School of Medicine, New Orleans 70112.
McCombs
C C
CC
Michalski
J P
JP
D000906:Antibodies / Q000276:immunology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000502:physiology; D018801:CD2 Antigens; D004306:Dose-Response Relationship, Immunologic; D004987:Ethers / Q000494:pharmacology; D000949:Histocompatibility Antigens Class II / Q000502:physiology*; D006801:Humans; D015759:Ionomycin; D008213:Lymphocyte Activation; D010835:Phytohemagglutinins / Q000494:pharmacology; D011971:Receptors, Immunologic / Q000502:physiology; D013601:T-Lymphocytes / Q000276:immunology*; D001613:beta 2-Microglobulin / Q000276:immunology*
D000906:Antibodies; D000945:Antigens, Differentiation, T-Lymphocyte; D018801:CD2 Antigens; D004987:Ethers; D000949:Histocompatibility Antigens Class II; D010835:Phytohemagglutinins; D011971:Receptors, Immunologic; D001613:beta 2-Microglobulin; D015759:Ionomycin