Expression of v-src induces a myeloproliferative disease in bone-marrow-reconstituted mice.
A recombinant retrovirus, N-TK-src, was used to introduce the v-src oncogene into mouse hematopoietic cells. This vector efficiently expresses both the neo and v-src genes in different hematopoietic lineages in culture as well as in mice reconstituted with infected bone marrow cells. Expression of v-src had no dramatic effect on the proliferative and differentiative capacity of hematopoietic precursors when assayed in methyl cellulose cultures. However, in mice reconstituted with N-TK-src-infected bone marrow cells, expression of v-src leads to the rapid development of a severe myeloproliferative disease, characterized by splenomegaly, anemia, and a shift of hematopoiesis from the bone marrow to the spleen.
Keller
G
G
Basel Institute for Immunology, Switzerland.
Wagner
E F
EF
D000818:Animals; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D005822:Genetic Vectors; D006410:Hematopoiesis; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000473:pathology; D051379:Mice; D008808:Mice, Inbred CBA; D009196:Myeloproliferative Disorders / Q000209:etiology* / Q000235:genetics / Q000473:pathology; D015688:Oncogene Protein pp60(v-src); D009857:Oncogenes; D011828:Radiation Chimera; D011993:Recombinant Fusion Proteins / Q000502:physiology; D012191:Retroviridae Proteins / Q000235:genetics / Q000502:physiology*
D011993:Recombinant Fusion Proteins; D012191:Retroviridae Proteins; D015688:Oncogene Protein pp60(v-src)