Effects of L-carnitine and palmitoylcarnitine on membrane fluidity of human erythrocytes.
Amphiphilic compounds such as long-chain acyl carnitine accumulate in ischemic myocardium and potentially contribute to the myocardial damage, and the role of carnitine in protecting the heart against ischemic damage is interesting. It has been reported that palmitoylcarnitine causes alterations in the membrane molecular dynamics, so this study was designed to investigate whether L-carnitine had a stabilizing effect of membrane fluidity using the spin-label technique. Human erythrocytes were spin-labeled with 5-doxylstearic acids, and membrane fluidity was quantified by measuring the change in the order parameter S. The administration of palmitoylcarnitine (100 microM) altered the membrane fluidity of erythrocytes and caused significant morphological changes. L-carnitine (2mM) decreased the alteration of the fluidity of erythrocytes incubated with palmitoylcarnitine (100 microM), and improved the morphological changes in erythrocytes. These results show that L-carnitine has a stabilizing effect of membrane fluidity as a result of interaction with the palmitoylcarnitine which has a detergent effect.
Kobayashi
A
A
Third Department of Internal Medicine, Hamamatsu University School of Medicine, Japan.
Watanabe
H
H
Fujisawa
S
S
Yamamoto
T
T
Yamazaki
N
N
D002331:Carnitine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D004578:Electron Spin Resonance Spectroscopy; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D007700:Kinetics; D008560:Membrane Fluidity / Q000187:drug effects*; D008855:Microscopy, Electron, Scanning; D010172:Palmitoylcarnitine / Q000494:pharmacology*
D010172:Palmitoylcarnitine; D002331:Carnitine