The Ad5 12S growth factor induces F9 cell proliferation and differentiation.
The 12S gene of the E1A region of adenovirus induces the production of a growth factor that is necessary for the immortalization of primary epithelial cells. This growth factor is able to replace the strict serum requirement of F9 cells, a teratocarcinoma-derived embryonal carcinoma cell line. Furthermore, the growth factor induces a morphological alteration in the F9 cells such that they resemble visceral endoderm cells and express the surface antigens, SSEA-1 and SSEA-3, characteristic of these differentiated cells.
Quinlan
M P
MP
Cold Spring Harbor Laboratory, New York 11724.
D000260:Adenoviruses, Human / Q000502:physiology*; D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D002454:Cell Differentiation; D002455:Cell Division; D005455:Fluorescent Antibody Technique; D006017:Glycolipids / Q000032:analysis; D006133:Growth Substances / Q000235:genetics / Q000494:pharmacology*; D066298:In Vitro Techniques; D016256:Lewis X Antigen; D051379:Mice; D013724:Teratoma / Q000473:pathology*; D014407:Tumor Cells, Cultured
D000954:Antigens, Surface; D006017:Glycolipids; D006133:Growth Substances; D016256:Lewis X Antigen