A region in the coding sequence is required for high-level expression of murine histone H3 gene.
Replication-dependent histone genes are expressed at high rates in S phase to provide the histone proteins required for chromosomal replication. Two genes, an H2a and H3 gene, located on chromosome 3 in the mouse and cloned together in a single 3-kilobase (plasmid MM614) restriction fragment are highly expressed. By transfecting mouse histone gene constructs into Chinese hamster ovary cells, we have identified a 110-nucleotide region within the coding sequence of the H3.2-614 gene that is required for high-level expression. Deletion of this region reduces expression of the gene by 20-fold. Additionally, the histone-coding region activates the human alpha-globin promoter, which is normally not expressed well in Chinese hamster ovary cells. Similar results with deletion constructions involving the H2a-614 gene suggest that an intragenic region plays an important role in transcription of these genes.
Hurt
M M
MM
Department of Chemistry, Florida State University, Tallahassee 32306.
Pandey
N B
NB
Marzluff
W F
WF
D000818:Animals; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000235:genetics; D002460:Cell Line; D002678:Chimera; D002872:Chromosome Deletion; D005796:Genes; D005801:Genes, Homeobox; D006657:Histones / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D015675:Osteocalcin; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014162:Transfection
D002135:Calcium-Binding Proteins; D006657:Histones; D012333:RNA, Messenger; D015675:Osteocalcin