Infectious cDNA clones of the DA strain of Theiler's murine encephalomyelitis virus.
The DA strain and other members of the TO subgroup of Theiler's murine encephalomyelitis viruses cause a persistent demyelinating infection, whereas the GDVII strain and other GDVII subgroup strains cause an acute lethal polioencephalomyelitis. We generated an infectious DA cDNA clone inserted into a transcription vector. Virus derived from transfection of transcripts produced a demyelinating disease indistinguishable from that of wild-type virus. The infectious clone provides a critical reagent for the production of interstrain recombinant viruses to help identify genetic loci responsible for the biological activities of the strains.
Roos
R P
RP
Department of Neurology, University of Chicago Pritzker School of Medicine, Illinois 60637.
Stein
S
S
Ohara
Y
Y
Fu
J L
JL
Semler
B L
BL
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D004770:Enterovirus / Q000235:genetics*; D005822:Genetic Vectors; D007739:L Cells / Q000382:microbiology; D004680:Maus Elberfeld virus / Q000235:genetics* / Q000472:pathogenicity; D051379:Mice; D008969:Molecular Sequence Data; D010957:Plasmids; D015183:Restriction Mapping; D013116:Spinal Cord / Q000382:microbiology / Q000473:pathology; D014158:Transcription, Genetic; D014162:Transfection; D014774:Virulence
D004279:DNA, Viral