Enhancement of herpes simplex virus type 2 (HSV-2) DNA synthesis in infected cells that constitutively express the BglII-N region of the HSV-2 genome.
The BglII-N fragment of the herpes simplex virus type-2 (HSV-2) genome encodes one of two known transforming regions of this DNA virus. In this study, we report the derivation of HeLa S3 cells (2DC4) that stably express the HSV-2 BglII-N region, including the small subunit of HSV-2 ribonucleotide reductase (RR). Superinfection of the 2DC4 cells with wild-type HSV-2 resulted in the efficient induction of HSV-2-encoded ICP10, DNA polymerase, and thymidine kinase. The amount of HSV-2 DNA synthesis in 8-hr HSV-2-infected 2DC4 cells was enhanced 2.6 +/- 0.6-fold relative to infected control cells. Furthermore, the replication kinetics of HSV-2 DNA in 2DC4 cells were accelerated relative to HeLa S3 cells; HSV-2 DNA synthesis was detectable as early as 3 hr postinfection in 2DC4 cells as compared to 6 hr postinfection in HeLa S3 cells. These results suggest that the BglII-N region of HSV-2 encodes function(s) that activate the viral DNA synthesis apparatus and that this activation could relate to the transforming ability of this DNA region.
Lee
P G
PG
Institute of Biomedical Sciences, Academia Sinica, Tapei, Republic of China.
Chang
J Y
JY
Yen
M S
MS
Cheng
Y C
YC
Nutter
L M
LM
D001483:Base Sequence; D002460:Cell Line; D002472:Cell Transformation, Viral; D002498:Centrifugation; D004279:DNA, Viral / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D015151:Immunoblotting; D012264:Ribonucleotide Reductases / Q000235:genetics; D018139:Simplexvirus / Q000235:genetics* / Q000502:physiology; D014162:Transfection; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000235:genetics; D014779:Virus Replication
D004279:DNA, Viral; D014764:Viral Proteins; D012264:Ribonucleotide Reductases