title: General characteristics and viral susceptibility of a newborn pig kidney (NPK) continuous culture.
abstract: We have developed a fibroblastic-like continuous culture of newborn pig kidney (NPK). The current cell line was serially passaged 160 times and appeared to be well suited for production and assay of a number of viruses affecting pigs, such as pig parvovirus, pseudorabies and transmissible gastroenteritis. The cell line appeared aneuploid, with a modal chromosome number of 36 and induced tumors, classified as fibrosarcoma, in athymic mice.
authors.affiliation: Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia, Brescia, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Ferrari
authors.forename: M N
authors.initials: MN
authors.lastname: Losio
authors.forename: G L
authors.initials: GL
authors.lastname: Gualandi
authors.forename: R
authors.initials: R
authors.lastname: Di Lernia
authors.forename: I
authors.initials: I
authors.lastname: Manocchio
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002460:Cell Line; D002470:Cell Survival; D006692:Classical Swine Fever Virus / Q000254:growth & development; D003588:Cytopathogenic Effect, Viral; D004771:Enteroviruses, Porcine / Q000254:growth & development; D005347:Fibroblasts; D011558:Herpesvirus 1, Suid / Q000254:growth & development; D007668:Kidney / Q000166:cytology*; D051379:Mice; D010321:Parvoviridae / Q000254:growth & development; D013552:Swine; D005760:Transmissible gastroenteritis virus / Q000254:growth & development; D014776:Virus Cultivation