Human fibroblasts release reactive oxygen species in response to interleukin-1 or tumour necrosis factor-alpha.
Human fibroblasts in primary culture released reactive oxygen species upon stimulation with cytokines such as interleukin-1 alpha (IL-1) or tumour necrosis factor-alpha (TNF). The primary radical produced was O2.- as determined by e.s.r. spin trapping and cytochrome c reduction. In contrast to the oxidative burst in granulocytes and monocytes, radical formation took place continuously for at least 4 h. Low-level chemiluminescence was increased by stimulation with IL-1 and TNF. Spectral characteristics and tests with azide led to the conclusion that the photoemissive species were excited carbonyls and not singlet oxygen. Further, there was a liberation of ethane from the cells. Radical production and light emission were not altered by either xanthine or allopurinol, nor by azide, cyanide or rotenone. O2.- production increased in the presence of NADH or NADPH, making an NAD(P)H oxidase a likely source.
Meier
B
B
Chemisches Institut, Tierärztliche Hochschule Hannover, Federal Republic of Germany.
Radeke
H H
HH
Selle
S
S
Younes
M
M
Sies
H
H
Resch
K
K
Habermehl
G G
GG
D003574:Cytochrome c Group / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D004980:Ethane / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism*; D005609:Free Radicals; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007375:Interleukin-1 / Q000494:pharmacology*; D007700:Kinetics; D008163:Luminescent Measurements; D009243:NAD / Q000494:pharmacology; D009249:NADP / Q000494:pharmacology; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D003574:Cytochrome c Group; D005609:Free Radicals; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D009243:NAD; D013481:Superoxides; D009249:NADP; D006861:Hydrogen Peroxide; D004980:Ethane; D010100:Oxygen