E-series prostaglandins activate cAMP-mediated potassium currents in follicle-enclosed Xenopus oocyte.
Receptor-mediated responses to prostaglandins E1 and E2 are shown by electrophysiological methods in follicle-enclosed oocytes of Xenopus laevis. In voltage-clamped oocytes, prostaglandins E1 and E2 elicited an outward hyperpolarizing current. This outward membrane current was caused by an increase in K+ conductance. The prostaglandin-induced current was augmented by adenylate cyclase activator, forskolin, and by phosphodiesterase inhibitor, theophylline, indicating that adenosine 3', 5'-cyclic monophosphate (cAMP) is involved in activating the K+ current. The prostaglandin responses were either abolished or greatly reduced by removing follicular cells with collagenase, suggesting that prostaglandin receptors reside in the follicular cells.
Mori
K
K
Department of Neuroscience, Osaka Bioscience Institute, Japan.
Oka
S
S
Tani
A
A
Ito
S
S
Watanabe
Y
Y
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000502:physiology; D004553:Electric Conductivity; D005260:Female; D008564:Membrane Potentials / Q000187:drug effects; D009865:Oocytes / Q000502:physiology*; D010053:Ovary / Q000502:physiology*; D011188:Potassium / Q000502:physiology*; D015221:Potassium Channels / Q000502:physiology*; D011458:Prostaglandins E / Q000494:pharmacology*; D011982:Receptors, Prostaglandin / Q000502:physiology*; D018078:Receptors, Prostaglandin E; D013806:Theophylline / Q000494:pharmacology; D014982:Xenopus laevis
D015221:Potassium Channels; D011458:Prostaglandins E; D011982:Receptors, Prostaglandin; D018078:Receptors, Prostaglandin E; D005576:Colforsin; D013806:Theophylline; D000242:Cyclic AMP; D000109:Acetylcholine; D011188:Potassium