The carbachol-induced enhancement of desynchronized sleep signs is dose dependent and antagonized by centrally administered atropine.
Considerable data show that microinjection of carbachol into the pontine reticular formation produces a desynchronized (D) sleep-like state. The present study examined the hypothesis that this carbachol-induced enhancement of D sleep signs is mediated by muscarinic, cholinergic receptors. This hypothesis was tested by quantifying the dose-dependent effects of centrally administered carbachol on the D sleep-like state and by pretreating the animals with centrally administered atropine. Six dosages of carbachol were microinjected into the pontine reticular formation of conscious cats and polygraphic measures of behavioral state were recorded. The percentage, latency, duration, frequency, and time course of the carbachol-induced D sleep-like state were dose dependent. Centrally administered atropine competitively antagonized the ability of carbachol to induce the D sleep-like state, whereas pontine administration of L-glutamate did not significantly alter D sleep. These data demonstrate that muscarinic, cholinergic receptors within the pontine reticular formation mediate the phenomenon of cholinoceptive D sleep sign enhancement.
Baghdoyan
H A
HA
Department of Anesthesia and Pharmacology, Pennsylvania State University, College of Medicine, Hershey 17033.
Lydic
R
R
Callaway
C W
CW
Hobson
J A
JA
D000818:Animals; D001285:Atropine / Q000494:pharmacology*; D001921:Brain; D002217:Carbachol / Q000008:administration & dosage / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D002415:Cats; D004305:Dose-Response Relationship, Drug; D005971:Glutamates / Q000494:pharmacology; D018698:Glutamic Acid; D008297:Male; D011149:Pons; D012895:Sleep, REM / Q000187:drug effects*
D005971:Glutamates; D018698:Glutamic Acid; D001285:Atropine; D002217:Carbachol