title: Basal body/centriolar DNA: molecular genetic studies in Chlamydomonas.
abstract: In Chlamydomonas reinhardtii, mutations on an unusual linkage group, the uni linkage group (ULG), affect structure and function of basal bodies. The ULG shows Mendelian segregation, but its genetic map is circular. Molecular cloning of fragments of the ULG was accomplished by taking advantage of restriction fragment length polymorphisms generated by crosses to Chlamydomonas smithii. These clones were used as probes to determine the size and form of the ULG chromosome; it is a 6-9 megabase linear molecule. Use of the probes for in situ DNA hybridization in cells localized the ULG chromosome to basal bodies.
authors.affiliation: Rockefeller University, New York 10021.
authors.forename: J L
authors.initials: JL
authors.lastname: Hall
authors.forename: Z
authors.initials: Z
authors.lastname: Ramanis
authors.forename: D J
authors.initials: DJ
authors.lastname: Luck
mesh_terms: D002502:Centrioles / Q000378:metabolism*; D002696:Chlamydomonas / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004270:DNA, Circular / Q000302:isolation & purification*; D005407:Flagella / Q000378:metabolism*; D008040:Genetic Linkage; D005819:Genetic Markers; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D004270:DNA, Circular; D005819:Genetic Markers