Development of a shuttle vector and a conjugative transfer system for Actinobacillus pleuropneumoniae.
An original genetic system for Actinobacillus pleuropneumoniae has been developed. A shuttle cloning vector, pYG53, was constructed from the wild-type plasmid pYG10. It permits, in conjunction with electroporation, the introduction of cloned genes into this species. A conjugal transfer system between Escherichia coli and A. pleuropneumoniae involving pYG54, a mobilizable derivative of pYG53, is also described. Conjugation efficiencies of 8.3 x 10(-3) exconjugants per donor can be obtained.
Lalonde
G
G
Department of Microbiology and Immunology, Stanford University School of Medicine, CA 94305.
O'Hanley
P D
PD
D003001:Cloning, Molecular / Q000379:methods; D003227:Conjugation, Genetic; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics*; D005822:Genetic Vectors; D006190:Haemophilus / Q000235:genetics*; D010957:Plasmids
D004262:DNA Restriction Enzymes