[The distribution of glutamate receptors in cultures of the motor area of the rat cerebral cortex studied by an immunoelectron microscopic method].
The aim of the study was to analyse the distribution and localization of glutamate receptors in the cultured cells of the neonatal rat motor cortex, using immunoelectron microscopic technique, and monoclonal antibodies preliminary labeled with colloidal gold. Monoclonal antibodies against glutamate-binding proteins of the adult rat cerebral cortex were produced by means of hybridization of immune splenocytes with plasmocytoma cells. It was found that monoclonal antibodies labeled with colloidal gold could reveal selectively the localization of glutamate receptors on the membranes of neurons. Glutamate receptors were detected on differentiating neuron membranes only, being absent from the glia cell surface.
Bobryshev
Iu V
IuV
Orlova
E A
EA
Balabanova
Iu V
IuV
Kuznetsov
A S
AS
Smirnova
T M
TM
D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal; D002454:Cell Differentiation; D046508:Culture Techniques; D005971:Glutamates / Q000378:metabolism*; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000378:metabolism; D008854:Microscopy, Electron; D009044:Motor Cortex / Q000378:metabolism / Q000648:ultrastructure*; D011485:Protein Binding; D051381:Rats; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter / Q000378:metabolism / Q000648:ultrastructure*; D013997:Time Factors
D000911:Antibodies, Monoclonal; D005971:Glutamates; D008565:Membrane Proteins; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter
Issledovanie raspredeleniia glutamatnykh retseptorov v kul'turakh motornoĭ zony kory golovnogo mozga krys immunoélektronno-mikroskopicheskim metodom.