A simple procedure for detecting proteins that bind preferentially to kDNA networks.
A procedure is described in which a fractionated protein extract from the trypanosome Crithidia fasciculata was incubated either with deproteinized C. fasciculata kDNA networks or nuclear DNA. At least three proteins in the range of 45-65 kDa had a specific affinity for the networks. We suggest that these proteins may play an important role in the biology of the kinetoplast.
Tittawella
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Department of Microbiology, University of Umeå, Sweden.
D000818:Animals; D011232:Chemical Precipitation; D002850:Chromatography, Gel / Q000379:methods; D003421:Crithidia / Q000032:analysis*; D004270:DNA, Circular / Q000378:metabolism*; D018105:DNA, Kinetoplast; D004268:DNA-Binding Proteins / Q000378:metabolism*
D004270:DNA, Circular; D018105:DNA, Kinetoplast; D004268:DNA-Binding Proteins