HSV infection induces increased transcription of Alu repeated sequences by RNA polymerase III.
The Alu family of repeated sequences is transcribed by both RNA polymerase II and RNA polymerase III. In cells infected with HSV, transcription by polymerase III increases while transcription by polymerase II decreases. By using virus strains carrying mutations in the genes encoding individual regulatory proteins, we have shown that this effect is dependent upon the immediate-early protein ICP27 and occurs by a process distinct from those which regulate viral gene expression. This is the first example of increased transcription of endogenous cellular sequences by RNA polymerase III during infection with a DNA virus.
Jang
K L
KL
Department of Biochemistry, University College and Middlesex School of Medicine, London, England.
Latchman
D S
DS
D002467:Cell Nucleus / Q000378:metabolism; D002472:Cell Transformation, Viral; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D015967:Gene Expression Regulation, Viral; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D012319:RNA Polymerase II / Q000378:metabolism; D012320:RNA Polymerase III / Q000378:metabolism*; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D012091:Repetitive Sequences, Nucleic Acid; D018139:Simplexvirus / Q000235:genetics*; D014158:Transcription, Genetic
D012334:RNA, Neoplasm; D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases; D012320:RNA Polymerase III