[Isolation of coronaviruses in cell culture from nasal swab samples of calves with respiratory tract diseases in the Federal Republic of Germany].
Coronavirus was isolated in HRT-18 tissue culture from nasal swabs of 4 from 15 calves showing respiratory symptoms. The strains showed cytopathic effects and could be identified as coronaviruses of bovine origin by their mouse erythrocyte agglutinating activity as well as serological specific hemagglutination inhibition and electron microscopical methods. This is the first report on the isolation of coronaviruses in tissue culture from the respiratory tract of calves in the FRG.
Jiménez
C
C
Herbst
W
W
Biermann
U
U
Müller
J M
JM
Schliesser
T
T
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D002478:Cells, Cultured; D003332:Coronaviridae / Q000302:isolation & purification*; D003333:Coronaviridae Infections / Q000382:microbiology / Q000662:veterinary*; D003588:Cytopathogenic Effect, Viral; D009296:Nasal Cavity / Q000382:microbiology*; D012141:Respiratory Tract Infections / Q000382:microbiology / Q000662:veterinary*
Isolierung von Coronaviren in der Zellkultur aus Nasentupferproben atemwegskranker Kälber in der Bundesrepublik Deutschland.