[The culturing of human Schwann cells: a new contribution to the study of polyneuropathy].
We have established 44 Schwann cell cultures from human peripheral nerves that underwent biopsy for diagnostic purposes using a new "sandwich" connective tissue and cut into 1 mm cubic explants which were placed between two glass coverslips coated with D-poly-L-lysine, in HAM-F10 medium, 15% FCS, 600 mg/dl glucose and antibiotics. In the first 3 weeks this new sandwich technique yields a fairly great and homogeneous amount of Schwann cell growth, with only a few fibroblasts and virtually no macrophages and provides a suitable substrate on which immuno cytochemical studies could be performed.
de Martini
I
I
Cadoni
A
A
Zicca
A
A
Mancardi
G L
GL
Bianchini
D
D
Zaccheo
D
D
D000954:Antigens, Surface / Q000032:analysis; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods*; D006801:Humans; D010523:Peripheral Nervous System Diseases / Q000473:pathology*; D012583:Schwann Cells / Q000276:immunology / Q000473:pathology*
D000954:Antigens, Surface
La coltura delle cellule di Schwann umane: un nuovo apporto per lo studio delle polineuropatie.