Cell proteins bind to multiple sites within the 5' untranslated region of poliovirus RNA.
The 5' noncoding region of poliovirus RNA contains sequences necessary for translation and replication. These functions are probably carried out by recognition of poliovirus RNA by cellular and/or viral proteins. Using a mobility-shift electrophoresis assay and 1,10-phenanthroline/Cu+ footprinting, we demonstrate specific binding of cytoplasmic factors with a sequence from nucleotides 510-629 within the 5' untranslated region (UTR). Complex formation was also observed with a second sequence (nucleotides 97-182) within the 5' UTR. These two regions of the 5' UTR appear to be recognized by distinct cell factors as determined by competition analysis and the effects of ionic strength on complex formation. However, both complexes contain eukaryotic initiation factor 2 alpha, as revealed by their reaction with specific antibody.
del Angel
R M
RM
Departmento de Genética y Biologia Molecular, Centro de Investigación y de Estudios Avanzados, Mexico City, Distrito Federal.
Papavassiliou
A G
AG
Fernández-Tomás
C
C
Silverstein
S J
SJ
Racaniello
V R
VR
D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism*; D009710:Nucleotide Mapping; D017955:Poliovirus / Q000235:genetics*; D011485:Protein Binding; D012367:RNA, Viral / Q000096:biosynthesis / Q000378:metabolism*; D013698:Templates, Genetic
D009363:Neoplasm Proteins; D012367:RNA, Viral