Biochemical characterization of the fibronectin binding sites for IgG.
Plasma fibronectin (Fn) is a constituent of cryoglobulins and has been shown to interact with immune complexes. In a previous report we demonstrated that Fn specifically bound to IgG immobilized on a solid matrix. To localize and biochemically characterize the sites on the Fn molecule involved in this interaction, Fn was enzymatically cleaved with subtilisin and subjected to IgG affinity chromatography. Three major polypeptide fragments of 16 kDa, 22 kDa, and a triplet of 26- to 29-kDa bound IgG. They were localized to three separate regions of the molecule by Western blot analysis using antisera to specific regions of the Fn molecule, by amino acid sequencing, and by their previously described heparin binding affinities. The 22-kDa fragment interacted with IgG under physiologic conditions and it is localized at the N-terminal of the Fn molecule. The 16-kDa and 26- to 29-kDa fragments bound to IgG under conditions of lower ionic strength; the former commences at residue 588, carboxyl-terminal to the collagen binding region and the latter begins at residue 1597, carboxyl-terminal to the cell binding domain. The interaction of Fn with Ig has significant implications in host defense and also in immune complex disease where basement membrane Fn may sequester immune complexes from the circulation.
Rostagno
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AA
Department of Pathology, New York University Medical Center 10016.
Frangione
B
B
Gold
L I
LI
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D005353:Fibronectins / Q000276:immunology / Q000378:metabolism*; D006493:Heparin / Q000378:metabolism; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011956:Receptors, Cell Surface; D011961:Receptors, Fc; D017469:Receptors, Fibronectin; D017452:Receptors, IgG; D011971:Receptors, Immunologic; D013381:Subtilisins
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D005353:Fibronectins; D007074:Immunoglobulin G; D011956:Receptors, Cell Surface; D011961:Receptors, Fc; D017469:Receptors, Fibronectin; D017452:Receptors, IgG; D011971:Receptors, Immunologic; C037680:heparin receptor; D006493:Heparin; D013381:Subtilisins