Respiratory-competent yeast mitochondrial DNAs generated by deleting intergenic regions.
Two respiratory-competent yeast strains having mitochondrial (mt) DNA characterized by single non-overlapping deletions, encompassing intergenic sequences, have been crossed. Diploid daughter clones have been screened by electrophoresis of mtDNA fragments, and a respiratory-competent clone (ER8.75), having a recombinant small mtDNA with both parental deletions, has been detected. ER8.75 mtDNA lacks around 20% of wild-type intergenic sequences, encompassing three ori/rep sequences. This mutant could be helpful in analyzing the organelle genome and, particularly, the function of intergenic sequences.
Piskur
J
J
Research School of Biological Sciences, Australian National University, Canberra.
D002872:Chromosome Deletion; D003433:Crosses, Genetic; D004262:DNA Restriction Enzymes; D004272:DNA, Mitochondrial / Q000032:analysis* / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D004272:DNA, Mitochondrial; D004262:DNA Restriction Enzymes