Expression of proliferating cell nuclear antigen/cyclin in human keratinocytes.
The expression of proliferating cell nuclear antigen (PCNA), also called cyclin, in human keratinocytes was examined by using the serum obtained from a SLE patient and a murine monoclonal antibody against PCNA/cyclin. In the normal epidermis, few of the nuclei were labeled with anti-PCNA/cyclin. This was in contrast to the positive nuclear staining seen in active lesions of psoriasis. In a primary culture of human keratinocytes growing as a monolayer, 20%-30% of cells expressed PCNA/cyclin. SV40-transformed human keratinocytes showed positive nuclear staining in about 40% of the cell population. In stratified keratinocytes cultured in a high Ca++ medium, PCNA/cyclin expression was decreased and only the cells in the basal and suprabasal layers showed positive staining. These results indicate that the expression of PCNA/cyclin correlates with the proliferating state in human keratinocytes and may not be associated with the mechanism of differentiation in keratinocytes.
Miyagawa
S
S
Department of Dermatology, Nara Medical University, Japan.
Okada
N
N
Takasaki
Y
Y
Iida
T
T
Kitano
Y
Y
Yoshikawa
K
K
Sakamoto
K
K
Steinberg
M L
ML
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D002453:Cell Cycle; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D006801:Humans; D066298:In Vitro Techniques; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen; D013539:Simian virus 40
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen