Porcine adrenal prolactin receptors: characterization, changes during neonatal development and effects of hypoprolactinemia.
1. Adrenal prolactin (PRL) receptors were identified within the adrenal cortex of pigs (Sus domesticus), and found to be located specifically on isolated zona fasciculata/reticularis cells (6437 sites per cell). 2. These PRL receptors were associated with binding to [125I]-oPRL which was characterized as being time and temperature dependent, specific for PRL, saturable, of high affinity (Ka = 10(10)/M) with a single class of binding sites, and irreversible except under extreme conditions. 3. The concentrations (fmol/mg protein) of PRL receptors decreased by 35% (P less than 0.05) between 3 and 10 days of age, and subsequently remained constant until 30 days of age. Total content (fmol/paired adrenals) increased progressively (2-fold, P less than 0.05) between 3 and 30 days of age. 4. Short-term (less than 16 hr) and prolonged (7 weeks) hypoprolactinemia (46-64% of control levels, P less than 0.05) were not associated with changes in numbers of porcine adrenal unoccupied PRL receptors.
Klemcke
H G
HG
US Department of Agriculture, US Meat Animal Research Center, Clay Center, NE 68933.
Pond
W G
WG
Nienaber
J A
JA
D000302:Adrenal Cortex / Q000378:metabolism; D000311:Adrenal Glands / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development / Q000378:metabolism*; D003080:Cold Temperature; D008297:Male; D008426:Maternal Deprivation; D011388:Prolactin / Q000097:blood*; D011981:Receptors, Prolactin / Q000378:metabolism*; D013312:Stress, Physiological / Q000378:metabolism; D013552:Swine
D011981:Receptors, Prolactin; D011388:Prolactin