Regulation of intercellular adhesion molecule-1 expression on endothelial cells with correlation to lymphocyte-endothelial binding.
Intercellular adhesion molecule-1 (ICAM-1) expression on cultured human umbilical cord vein endothelial cells (EC) and its correlation to the leucocyte adherence to EC was investigated. Gamma interferon (IFN-gamma) increases the ICAM-1 expression on EC and concomitantly also increases the binding of leucocytes to EC in vitro. Methylprednisolone down-regulates the IFN-gamma-induced binding, but does not alter the ICAM-1 expression. Leukotriene B4 (LTB4) rapidly increases the binding of lymphocytes to EC, but does not induce ICAM-1 expression on the EC. The dissociation between ICAM-1 expression and leucocyte binding to EC clearly indicates that ICAM-1 is not the only ligand.
Renkonen
R
R
Transplantation Laboratory, University of Helsinki, Finland.
D000943:Antigens, Differentiation / Q000378:metabolism; D000954:Antigens, Surface / Q000096:biosynthesis*; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000378:metabolism*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D007975:Leukotriene B4 / Q000494:pharmacology; D016169:Lymphocyte Function-Associated Antigen-1; D008214:Lymphocytes / Q000502:physiology*
D000943:Antigens, Differentiation; D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D016169:Lymphocyte Function-Associated Antigen-1; D007975:Leukotriene B4; D007371:Interferon-gamma