Competitive peptide antagonists of ANF-induced cyclic guanosine monophosphate production.
Atrial natriuretic factor (isoleucine ANF 101-126), cleaved ANF (isoleucine ANF 101-105/106-126) and des (Gln 18, Ser 19, Gly 20, Leu 21, Gly 22) ANF 4-23-NH2 (C-ANF 4-23) stimulated cyclic guanosine monophosphate production (cGMP) by rat aortic vascular smooth muscle cells (VSMC) in culture. Cleaved ANF and ANF C4-23 also antagonised or diminished the response to ANF 101-126. Agonist and antagonist actions of both peptides were dose-related. In contrast, prepro ANF (104-123), an ANF precursor fragment, exhibited no agonist or antagonist effect on cGMP production.
Abell
T J
TJ
Department of Medicine, Princess Margaret Hospital, Christchurch, New Zealand.
Richards
A M
AM
Yandle
T G
TG
Espiner
E A
EA
Frampton
C
C
Ikram
H
H
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism / Q000494:pharmacology*; D002478:Cells, Cultured; D006152:Cyclic GMP / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism; D011498:Protein Precursors / Q000378:metabolism; D051381:Rats; D011921:Rats, Inbred WKY
D011498:Protein Precursors; D009320:Atrial Natriuretic Factor; D006152:Cyclic GMP