title: Evaluation of the Du Pont HERPCHEK herpes simplex virus antigen test with clinical specimens.
abstract: The HERPCHEK herpes simplex virus (HSV) antigen test (Du Pont, Billerica, Mass.) is a biotinstreptavidin-amplified enzyme-linked immunoassay for the detection of HSV antigen directly in clinical specimens. In a total of 200 mucocutaneous specimens, predominantly lesions from nongenital sites, HERPCHEK had a sensitivity of 90% and a specificity of 99% compared with the shell vial tissue culture method which uses primary rabbit kidney cells. Five false-negative results by HERPCHEK were found with specimens that contained a low percentage of positive cells by the direct immunoperoxidase stain used in a tissue culture method (1 to 9%). The absorbance values of the clinical specimens by the HERPCHEK test do not correlate well with the percentage of positive cells. Our results indicate that the HERPCHEK HSV antigen test, when performed with specimens from lesions, is a sensitive and specific test as compared with shell vial culture.
authors.affiliation: Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892.
authors.forename: T C
authors.initials: TC
authors.lastname: Wu
authors.forename: S
authors.initials: S
authors.lastname: Zaza
authors.forename: J
authors.initials: J
authors.lastname: Callaway
mesh_terms: D000956:Antigens, Viral / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005188:False Negative Reactions; D005260:Female; D006561:Herpes Simplex / Q000175:diagnosis*; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D018139:Simplexvirus / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D000956:Antigens, Viral; D011933:Reagent Kits, Diagnostic