title: Production of endothelin 1 by cultured bovine retinal endothelial cells and presence of endothelin receptors on associated pericytes.
abstract: Endothelinlike immunoreactivity was detected by radioimmunoassay in medium conditioned by cultured endothelial cells obtained from bovine retinal microvessels (9.2 +/- 6.5 pM, n = 4). Sephadex G-25 column chromatography and fast-protein liquid chromatography revealed that most of the endothelinlike immunoreactivity was eluted in an identical position to synthetic endothelin 1. Retinal capillary pericyte-conditioned medium contained 2.9 pM endothelinlike immunoreactivity. In contrast to endothelial cells, retinal pericytes were found to bind endothelin. The dissociation constant and binding capacity were 0.14 nM and 1.5 x 10(5) sites/cell (n = 3), respectively. These findings suggest that endothelin produced by the retinal endothelial cells binds to the pericytes, adding support to the suggestion that pericytes in the retina may have a musclelike function.
authors.affiliation: Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, London, United Kingdom.
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
authors.forename: R A
authors.initials: RA
authors.lastname: Brooks
authors.forename: S M
authors.initials: SM
authors.lastname: Kanse
authors.forename: M A
authors.initials: MA
authors.lastname: Ghatei
authors.forename: E M
authors.initials: EM
authors.lastname: Kohner
authors.forename: S R
authors.initials: SR
authors.lastname: Bloom
mesh_terms: D000818:Animals; D001665:Binding Sites; D002196:Capillaries / Q000166:cytology / Q000378:metabolism; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D016232:Endothelins; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D007150:Immunohistochemistry; D010452:Peptide Biosynthesis; D010455:Peptides / Q000032:analysis; D011863:Radioimmunoassay; D011956:Receptors, Cell Surface / Q000032:analysis / Q000378:metabolism*; D017466:Receptors, Endothelin; D012160:Retina / Q000166:cytology / Q000378:metabolism*; D012171:Retinal Vessels / Q000166:cytology / Q000378:metabolism*
chemical_list: D016232:Endothelins; D010455:Peptides; D011956:Receptors, Cell Surface; D017466:Receptors, Endothelin