title: Mapping of chromosomal loci associated with lipopolysaccharide synthesis and serotype specificity in Vibrio cholerae 01 by transposon mutagenesis using Tn5 and Tn2680.
abstract: Vibrio cholerae strains of the 01 serotype have been classified into three subclasses, Ogawa, Inaba and Hikojima, which are associated with the O-antigen of the lipopolysaccharide (LPS). The DNA encoding the biosynthesis of the O-antigen, the rfb locus, has been cloned and analysed (Manning et al. 1986; Ward et al. 1987). Transposon mutagenesis of the Inaba and Ogawa strains of V. cholerae, using Tn5 or Tn2680 allowed the isolation of a series of independent mutants in each of these serotypes. Some of the insertions were mapped to the rfb region by Southern hybridization using the cloned rfb DNA as a probe, confirming this location to be responsible for both O-antigen production and serotype specificity. The other insertions allowed a second region to be identified which is involved in V. cholerae LPS biosynthesis.
authors.affiliation: Department of Microbiology and Immunology, University of Adelaide, Australia.
authors.forename: H M
authors.initials: HM
authors.lastname: Ward
authors.forename: P A
authors.initials: PA
authors.lastname: Manning
mesh_terms: D001435:Bacteriophages / Q000235:genetics; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D004251:DNA Transposable Elements; D008070:Lipopolysaccharides / Q000096:biosynthesis / Q000235:genetics*; D009154:Mutation; D015183:Restriction Mapping; D012703:Serotyping; D014734:Vibrio cholerae / Q000145:classification / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D008070:Lipopolysaccharides