Identification of a dihydropyridine-sensitive calcium channel in chick brain by a monoclonal antibody.
A Balb/c mouse was immunized with chick synaptic plasma membranes and monoclonal antibodies were produced by fusion of spleen cells with NS-1 mouse myeloma cells. One antibody, MAC-L1, immunoprecipitated more than 90% of the [3H]PN200-110-labeled calcium channels but only 20% of the omega -conotoxin receptor solubilized from the chick brain membranes. Thus possibly, a certain portion of the omega -conotoxin receptor in the chick brain is a dihydropyridine-sensitive calcium channel. By the specific immunoprecipitation of 125I-labeled proteins, two large polypeptides of 193kDa and 130kDa under reducing conditions were identified as the major components of the calcium channel.
Takahashi
M
M
Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
Fujimoto
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal; D001345:Autoradiography; D001921:Brain / Q000378:metabolism*; D015220:Calcium Channels / Q000032:analysis*; D002645:Chickens; D004095:Dihydropyridines / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D051379:Mice; D008807:Mice, Inbred BALB C; D011233:Precipitin Tests; D017981:Receptors, Neurotransmitter / Q000187:drug effects*
D000911:Antibodies, Monoclonal; D015220:Calcium Channels; D004095:Dihydropyridines; D017981:Receptors, Neurotransmitter; C053157:omega-conotoxin receptor