Eradication of adenovirus E1-induced tumors by E1A-specific cytotoxic T lymphocytes.
Cytotoxic T lymphocyte (CTL) clones against adenovirus type 5 (Ad5) early region 1 (E1)-transformed cells were generated in C57BL/6 (B6) mice. A defined peptide encoded by Ad5 E1A is the target structure for H-2Db-restricted CTLs. Upon intravenous injection into B6 nude mice bearing Ad5 E1-induced tumors, these CTLs, if combined with recombinant IL-2, destroy subcutaneous tumor masses up to 10 cm3. The in vivo action of CTLs is highly specific, and long-term "memory" persists in treated nude mice months after tumor regression. Our data show an important role for CTLs directed against a viral nuclear oncogene product in tumor eradication.
Kast
W M
WM
The Netherlands Cancer Institute, Amsterdam.
Offringa
R
R
Peters
P J
PJ
Voordouw
A C
AC
Meloen
R H
RH
van der Eb
A J
AJ
Melief
C J
CJ
D000257:Adenoviridae Infections / Q000276:immunology* / Q000628:therapy; D017456:Adenovirus Early Proteins; D000595:Amino Acid Sequence; D000818:Animals; D000957:Antigens, Viral, Tumor / Q000276:immunology*; D002478:Cells, Cultured; D002999:Clone Cells; D003602:Cytotoxicity, Immunologic; D015342:DNA Probes; D007167:Immunotherapy; D007694:Killer Cells, Natural / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D008819:Mice, Nude; D008969:Molecular Sequence Data; D009374:Neoplasms, Experimental / Q000276:immunology* / Q000628:therapy / Q000648:ultrastructure; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000276:immunology*; D010446:Peptide Fragments / Q000138:chemical synthesis; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000637:transplantation / Q000648:ultrastructure
D017456:Adenovirus Early Proteins; D000957:Antigens, Viral, Tumor; D015342:DNA Probes; D009856:Oncogene Proteins, Viral; D010446:Peptide Fragments