title: [In situ hybridization in detecting virus infections in biopsies following orthotopic liver transplantation].
abstract: Using the in-situ DNA hybridization we studied 62 liver biopsies from 34 patients for the presence of CMV-, EBV-, HBV-, and HSV-Genome. In the first biopsies positivity-rates were as follows: CMV 62%, EBV 71%, HBV 15%, HSV 15%. In 6 cases a conversion from a negative intranuclear CMV-status to a positive status was seen correlating with an increase in serological titers and with clinical signs of viral infection.
authors.affiliation: Pathologisches Institut, Medizinischen Hochschule Hannover.
authors.forename: C
authors.initials: C
authors.lastname: Wittekind
authors.forename: J
authors.initials: J
authors.lastname: Kemnitz
authors.forename: M
authors.initials: M
authors.lastname: Helmke
authors.forename: B
authors.initials: B
authors.lastname: Ringe
authors.forename: H
authors.initials: H
authors.lastname: Milbradt
authors.forename: T
authors.initials: T
authors.lastname: Thielke
authors.forename: A
authors.initials: A
authors.lastname: Georgii
mesh_terms: D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D001706:Biopsy; D002648:Child; D002675:Child, Preschool; D003587:Cytomegalovirus / Q000302:isolation & purification; D004279:DNA, Viral / Q000302:isolation & purification*; D006084:Graft Rejection; D006509:Hepatitis B / Q000473:pathology; D006515:Hepatitis B virus / Q000302:isolation & purification; D006525:Hepatitis, Viral, Human / Q000473:pathology*; D004854:Herpesvirus 4, Human / Q000302:isolation & purification; D006801:Humans; D008099:Liver / Q000473:pathology; D016031:Liver Transplantation; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D009894:Opportunistic Infections / Q000473:pathology*; D011183:Postoperative Complications / Q000473:pathology*; D018139:Simplexvirus / Q000302:isolation & purification
chemical_list: D004279:DNA, Viral
vernacular_title: In-situ-Hybridisation zur Erkennung von Virusinfektionen an Biopsien nach orthotoper Lebertransplantation.