title: Near infrared emission of singlet oxygen generated in the dark.
abstract: Singlet oxygen generation is reported from (1) enzymatic reaction and (2) electron transfer reactions of the superoxide anion measured directly with an ultrasensitive near-IR emission spectrophotometer by monitoring the O2(1 delta g)----O2 (3 sigma g-) transition at 1268 nm. Near-IR emission spectra from the myeloperoxidase and lactoperoxidase enzymatic systems show only emission of singlet oxygen at 1268 nm. The lipoxygenase/Na-linoleate enzymatic reaction exhibits two emissions, 1268 nm and 1288 nm. The latter emission is identified as originating from a peroxy radical. Spectral and kinetic data giving evidence of singlet oxygen generation is obtained from the reaction of potassium superoxide solubilized by 18-crown-6-ether in acetonitrile with a series of organometallic coordination compounds.
authors.affiliation: Department of Chemistry, Harvard University, Cambridge, MA 02138.
authors.forename: A U
authors.initials: AU
authors.lastname: Khan
mesh_terms: D002711:Chloride Peroxidase / Q000378:metabolism; D003624:Darkness; D004579:Electron Transport; D006801:Humans; D007107:Immune System / Q000378:metabolism; D007784:Lactoperoxidase / Q000378:metabolism; D008084:Lipoxygenase / Q000378:metabolism; D008163:Luminescent Measurements; D010100:Oxygen / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D026082:Singlet Oxygen; D013481:Superoxides / Q000378:metabolism
chemical_list: D013481:Superoxides; D026082:Singlet Oxygen; D007784:Lactoperoxidase; D002711:Chloride Peroxidase; D009195:Peroxidase; D008084:Lipoxygenase; D010100:Oxygen