title: [Design of a nutrient medium for bacteria of the genus Haemophilus--producers of restriction endonucleases].
abstract: A culture medium for the cultivation of hemophilic bacteria, containing acidic casein hydrolysate, aminopeptide and fodder yeast extract, has been proposed. The growth-stimulating properties of this medium have been studied on 5 strains producing restrictases differing in their specificity. In growing these producer strains in a model ANKUM-2 fermenter with the supply of carbohydrate substrates (glucose, sucrose, glycerin) the yield of biomass, considered to be high for hemophilic bacteria (10-14 g of humid substance from 1 liter of the medium), has been achieved. As shown on H. influenzae Rc B-2297 used as an example, an increase in the yield of microbial biomass leads to a decrease in restrictase specific activity.
authors.forename: V M
authors.initials: VM
authors.lastname: Poliachenko
authors.forename: V A
authors.initials: VA
authors.lastname: Mel'nikova
authors.forename: I M
authors.initials: IM
authors.lastname: Gruber
authors.forename: G A
authors.initials: GA
authors.lastname: Smirnova
authors.forename: B M
authors.initials: BM
authors.lastname: Raskin
mesh_terms: D001431:Bacteriological Techniques / Q000295:instrumentation; D003470:Culture Media / Q000378:metabolism*; D004262:DNA Restriction Enzymes / Q000096:biosynthesis*; D005285:Fermentation; D006190:Haemophilus / Q000201:enzymology / Q000254:growth & development*; D006193:Haemophilus influenzae / Q000201:enzymology / Q000254:growth & development; D008970:Molecular Weight
chemical_list: D003470:Culture Media; D004262:DNA Restriction Enzymes
vernacular_title: Konstruirovanie pitatel'noĭ sredy dlia bakteriĭ roda Haemophilus--produtsentov restriktiruiushchikh éndonukleaz.