Endocytosis in the rat retinal pigment epithelium.
Endocytosis in the retinal pigment epithelium (RPE) of rats was studied using horseradish peroxidase, microperoxidase and ferritin tracers. Tracer uptake was mediated by coated pits and coated vesicles. Coated pits formed at two discrete regions at the RPE plasma membrane: that portion of basal membrane directly opposing Bruch's membrane, and at the bases of the apical lamellae and villi. Two populations of coated vesicles were identified and distinguished by size, location and function. Large coated vesicles (91.8 +/- 14.7 nm in diameter) were located near the cell surface and incorporated tracer. Small coated vesicles (64.5 +/- 15.7 nm diameter) located more deeply within the cell were not tracer-labeled, and were often fused with the endoplasmic reticulum or the Golgi apparatus. Observations of the endocytic pathway in rat RPE cells are presented. Tracer was also found in organelles of the lysosomal system, e.g. the multivesicular body, but was not identified in the smooth endoplasmic reticulum or Golgi apparatus.
Perlman
J I
JI
Department of Ophthalmology, Montefiore Medical Center/Albert Einstein College of Medicine, Bronx, N.Y.
Piltz
J
J
Korte
G
G
Tsai
C
C
D000818:Animals; D003034:Coated Pits, Cell-Membrane / Q000648:ultrastructure; D004705:Endocytosis; D005260:Female; D006735:Horseradish Peroxidase; D002479:Inclusion Bodies / Q000648:ultrastructure; D008854:Microscopy, Electron; D010857:Pigment Epithelium of Eye / Q000166:cytology* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains
D006735:Horseradish Peroxidase