Erythropoietin. Receptor characteristics during the ontogeny of hamster yolk sac erythroid cells.
Erythropoietin (Epo) binds specifically to receptors on the surface membrane of responsive erythroid cells. In search of ontogenic changes in Epo receptor behavior, we studied characteristics of specific binding to hamster yolk sac erythroid cells during hamster ontogeny. We detected receptors specific for Epo on these cells throughout the duration of their intravascular existence (hamster gestational days 8 through 13). These receptors are saturable at an Epo concentration of 1.2 nM in the incubation medium. Attainment of equilibrium of binding prior to hormone internalization, a requirement for receptor binding assays, was possible at 10 degrees C but not at 37 degrees C. Hence, all incubations of cells with Epo were carried out at 10 degrees C. Data on specific binding analyzed by the method of Scatchard demonstrated that yolk sac erythroid cells possess a single class of Epo receptors at each stage of gestation examined. Binding affinity and numbers of receptors per cell change as ontogeny progresses: Kd (the dissociation constant) increases, a phenomenon observed in other differentiating cell systems, whereas the number of receptors per cell peaks on gestational day 10. The variability in number of receptors per cell is consonant with up and down regulation controlled by Epo availability. We propose that the progressive increase in Kd might be best explained by ontogenic changes in cell membrane structure contiguous to the receptors themselves.
Boussios
T
T
Hematology-Oncology Division, St. Luke's-Roosevelt Hospital Center, New York, New York 10025.
Bertles
J F
JF
Goldwasser
E
E
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D006224:Cricetinae; D004921:Erythropoietin / Q000378:metabolism*; D005333:Fetus; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D007700:Kinetics; D011956:Receptors, Cell Surface / Q000378:metabolism*; D017467:Receptors, Erythropoietin; D011994:Recombinant Proteins / Q000378:metabolism; D015017:Yolk Sac / Q000502:physiology*
D011956:Receptors, Cell Surface; D017467:Receptors, Erythropoietin; D011994:Recombinant Proteins; D004921:Erythropoietin