Secondary structural features of the bacteriophage Mu-encoded A and B transposition proteins.
The role of the bacteriophage Mu-encoded A and B proteins is to direct the transposition of Mu DNA. These are the first active DNA transposition proteins to have been purified and their mechanism of action at the biochemical level is under intensive study. Structural studies on these proteins, however, have lagged behind their biochemical characterization. We report here near- and far-u.v. c.d. spectra for these proteins and their secondary structural features derived from these data. The Mu A protein appears to be composed of primarily beta-sheet (40%) with 24% alpha-helix, 9% beta-turn and 27% random coil. In contrast, the Mu B protein contains 55% alpha-helix with only 13% beta-sheet and 3+ beta-turn and 29% random coil. The near-u.v. c.d. spectrum of the A protein was not unusual; however, the profile of the B protein suggested either buried or restricted chromophores within the protein or short-range interactions between aromatic residues.
Chaconas
G
G
Department of Biochemistry, University of Western Ontario, London.
McCubbin
W D
WD
Kay
C M
CM
D002942:Circular Dichroism; D003090:Coliphages / Q000235:genetics*; D004251:DNA Transposable Elements; D004279:DNA, Viral / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D009713:Nucleotidyltransferases / Q000235:genetics*; D010957:Plasmids; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D019895:Transposases; D014764:Viral Proteins / Q000235:genetics*
D004251:DNA Transposable Elements; D004279:DNA, Viral; D004268:DNA-Binding Proteins; C050938:Mu B protein, bacteriophage; D014764:Viral Proteins; D009713:Nucleotidyltransferases; D019895:Transposases