Gene mapping on mouse chromosome 8 by interspecific crosses: new data on a linkage group conserved on human chromosome 16q.
A large conserved linkage group exists on mouse chromosome 8 and human chromosome 16q, including the loci for chymotrypsinogen B (Ctrb), haptoglobin (Hp), lecithin:cholesterol acyltransferase (Lcat), metallothionein-1,-2 (Mt-1,-2), tyrosine aminotransferase (Tat), and uvomorulin (Um). Using cloned gene probes, these six loci were mapped in M. m. domesticus X M. spretus interspecific crosses relative to a number of chromosome 8 anchor loci resulting in the gene order Es-1,Es-9-Mt-1,-2-Got-2-Es-2,Es-7,Lcat,Um-Hp,Tat,Ctrb-e. These results complement earlier studies and redefine the conserved segment on mouse chromosome 8, previously defined by the Hp-Tat interval, by the 24-cM interval between Mt-1,-2 and the conserved locus for adenine phosphoribosyltransferase, Aprt, mapped at 25 cM from Es-1 by T. B. Nesterova, P. M. Borodin, S. M. Zakian, and O. L. Serov (1987, Biochem. Genet. 25: 563-568). Within this segment, the gene order appears the same in man and mouse. While map distances between HP-TAT,HP-CTRB, and TAT-CTRB of respectively 7, 11, and 9 cM have previously been measured in man, no crossovers between Hp, Tat, and Ctrb were observed in over 100 meioses in the mouse.
Scherer
G
G
Institute of Human Genetics, Freiburg, Federal Republic of Germany.
Bausch
E
E
Gaa
A
A
von Deimling
O
O
D000818:Animals; D015139:Blotting, Southern; D015820:Cadherins / Q000235:genetics; D002885:Chromosomes, Human, Pair 16; D002919:Chymotrypsinogen / Q000235:genetics; D003433:Crosses, Genetic; D015342:DNA Probes; D008040:Genetic Linkage; D006242:Haptoglobins / Q000235:genetics; D006801:Humans; D008668:Metallothionein / Q000235:genetics; D051379:Mice / Q000235:genetics*; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D011995:Recombination, Genetic; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014444:Tyrosine Transaminase / Q000235:genetics
D015820:Cadherins; D015342:DNA Probes; D006242:Haptoglobins; D002919:Chymotrypsinogen; D008668:Metallothionein; D007862:Phosphatidylcholine-Sterol O-Acyltransferase; D014444:Tyrosine Transaminase