Protein analysis of herpes simplex virus latency in vitro established with cycloheximide.
Herpes simplex virus (HSV)-specific protein synthesis was examined during establishment of HSV latency and reactivation of virus in human embryonic lung cells treated with cycloheximide and incubated at 40.5 degrees. Eight viral proteins, identified during the first two days of establishment of latency at 40.5 degrees, were undetectable by Day 3. At least two synthesized proteins were present during the maintenance phase of latency. Reactivation of HSV (viral protein 135K) was first detected in latently infected cultures between 2 and 3 hr after superinfection with human cytomegalovirus (HCMV). During this period an 82K protein with the same molecular weight as one of the HCMV immediate-early proteins (82 and 75K) was detected in the immunoprecipitates of latently infected cultures with anti-HSV serum. Thus, this HSV latency system can be used to analyze protein synthesis and clarify reactivation of HSV by HCMV superinfection.
Shiraki
K
K
Department of Microbiology and Immunology, Pennsylvania State University, College of Medicine, Hershey 17033.
Rapp
F
F
D000956:Antigens, Viral / Q000032:analysis; D015153:Blotting, Western; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D006561:Herpes Simplex / Q000503:physiopathology*; D006801:Humans; D066298:In Vitro Techniques; D008970:Molecular Weight; D018139:Simplexvirus / Q000254:growth & development* / Q000276:immunology; D013997:Time Factors; D014764:Viral Proteins / Q000096:biosynthesis / Q000276:immunology
D000956:Antigens, Viral; D014764:Viral Proteins; D003513:Cycloheximide