Uncoupling of receptor-mediated cellular responses by ionic lithium.
The propensity of ionic lithium to interfere with the coupling of receptors to guanine nucleotide binding proteins (G-proteins) has only recently been investigated using rat cortical membranes. In the present study we have used intact isolated osteoclasts to investigate lithium-induced uncoupling of the receptor-mediated actions of calcitonin. All actions of calcitonin on the osteoclast were abolished by ionic lithium. We believe that the cation prevents signal transduction by inhibiting G protein-receptor interaction, the first step in intracellular signalling.
Zaidi
M
M
Department of Chemical Pathology, Royal Postgraduate Medical School, Hammersmith Hospital, London.
Patchell
A
A
Datta
H K
HK
MacIntyre
I
I
D000818:Animals; D001862:Bone Resorption; D002116:Calcitonin / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D066298:In Vitro Techniques; D008094:Lithium / Q000494:pharmacology*; D010010:Osteoclasts / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D051381:Rats; D018003:Receptors, Calcitonin; D011956:Receptors, Cell Surface / Q000187:drug effects*
D018003:Receptors, Calcitonin; D011956:Receptors, Cell Surface; D002116:Calcitonin; D008094:Lithium