Rat liver gamma glutamyl transpeptidase mRNA differs in the 5' untranslated sequence from the corresponding kidney mRNA.
A clone containing the entire protein-coding region of gamma glutamyl transpeptidase (GGT) from ethoxyquin- (EQ) treated rat liver has been isolated from a cDNA library. DNA sequence analysis showed that the protein-coding and 3' untranslated regions are almost identical to rat kidney GGT, but the sequences differ in the 5' untranslated region. Southern blot data suggest a single gene for GGT in the rat, hence the different mRNAs may originate by differential transcriptional and/or posttranscriptional processing.
Griffiths
S A
SA
MRC Toxicology Unit, Carshalton, Surrey, U.K.
Manson
M M
MM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D005015:Ethoxyquin / Q000494:pharmacology; D005796:Genes; D007668:Kidney / Q000201:enzymology*; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000302:isolation & purification*; D051381:Rats; D011916:Rats, Inbred F344; D005723:gamma-Glutamyltransferase / Q000235:genetics*
D012333:RNA, Messenger; D004247:DNA; D005015:Ethoxyquin; D005723:gamma-Glutamyltransferase