Phosphoinositide-specific phospholipase C of a cloned mast cell line, mastocytoma P-815: purification and some characterization.
1. Multiple forms of phosphoinositide-phospholipase C (PLC) were isolated from mastocytoma; two cytosolic forms (cPLC-I, Mr 150,000; cPLC-II, Mr 110,000) and two membrane-associated forms (mPLC-I, Mr 85,000; mPLC-II, Mr 85,000). 2. Four PLC forms differently behaved in substrate specificity and effect of GTP-binding proteins.
Banno
Y
Y
Department of Biochemistry, Gifu University School of Medicine, Japan.
Kanoh
H
H
Nagata
K
K
Yada
Y
Y
Nozawa
Y
Y
D000818:Animals; D002118:Calcium / Q000502:physiology; D002461:Cell Line, Transformed; D002462:Cell Membrane / Q000032:analysis; D002999:Clone Cells; D003600:Cytosol / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000302:isolation & purification*; D008407:Mast Cells / Q000201:enzymology*; D012515:Mast-Cell Sarcoma / Q000201:enzymology; D051379:Mice; D008970:Molecular Weight; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010727:Phosphoric Diester Hydrolases / Q000302:isolation & purification*; D010738:Type C Phospholipases / Q000302:isolation & purification
D007527:Isoenzymes; D010727:Phosphoric Diester Hydrolases; D010738:Type C Phospholipases; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D002118:Calcium