title: Methyl prednisolone acetate modulation of infection and subsequent pulmonary pathology in hamsters exposed to parainfluenza-1 virus (Sendai).
abstract: Parainfluenza-1 virus (PI-1) has a wide host range; the disease process observed varies with the age, previous exposure, and species. This study was performed to determine possible effects of the corticosteroid methyl prednisolone acetate (MPA) on PI-1 infection in hamsters. Hamsters serologically negative for PI-1 were exposed to virus alone or were exposed to virus the day after pretreatment with a single subcutaneous injection of MPA. Serum antibodies to PI-1 were present in virus-only exposed hamsters by Day 8 and increased up to Day 20. PI-1 was recovered from lungs of virus-only exposed hamsters on Day 2 to 8. Virus antigen was detected by immunocytochemistry on Days 2 to 10 in lungs of virus-only exposed hamsters. Virus-associated lesions in these hamsters began as acute bronchiolar epithelium degeneration and necrosis on Day 4 and were foci of fibrosis by Days 12 to 20. Hamsters exposed to virus after MPA treatment developed no antibodies to virus, had no virus detectable by plaque assays or immunocytochemistry, and had no pulmonary lesions. Hamsters treated with MPA had decreased total lymphocyte counts up to Day 20 after treatment. Treatment of hamsters with MPA one day prior to PI-1 virus exposure is associated with no detectable evidence of viral infection. Humoral and cellular immunity mediated by MPA-sensitive lymphocytes may mediate some of the manifestations of PI-1 pulmonary disease.
authors.affiliation: Massachusetts Institute of Technology, Division of Comparative Medicine, Cambridge.
authors.forename: P B
authors.initials: PB
authors.lastname: Kimsey
authors.forename: M E
authors.initials: ME
authors.lastname: Goad
authors.forename: Z B
authors.initials: ZB
authors.lastname: Zhao
authors.forename: G
authors.initials: G
authors.lastname: Brackee
authors.forename: J G
authors.initials: JG
authors.lastname: Fox
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000032:analysis; D006224:Cricetinae; D004198:Disease Susceptibility; D004797:Enzyme-Linked Immunosorbent Assay; D007124:Immunoenzyme Techniques; D008168:Lung / Q000382:microbiology / Q000473:pathology*; D008297:Male; D008647:Mesocricetus; D008775:Methylprednisolone / Q000031:analogs & derivatives* / Q000494:pharmacology; D000077555:Methylprednisolone Acetate; D010222:Parainfluenza Virus 1, Human / Q000276:immunology / Q000302:isolation & purification; D018184:Paramyxoviridae Infections / Q000276:immunology* / Q000382:microbiology / Q000473:pathology
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D000077555:Methylprednisolone Acetate; D008775:Methylprednisolone