[Elaboration of an immunosorbent for the purification of porins from Salmonella typhi 9, 12, Vi:d].
The current work was undertaken to purify porins of Salmonella typhi, which are outer membrane proteins (OMPs) that induce protection in mice against challenge with the bacteria in mucin. OMPs, isolated with a non-ionic detergent, had a 4% contamination with LPS (endotoxin) and molecular sizes ranging from 17 to 70 KDa. Porins (Mw 38-41 KDa) were isolated from OMPs preparative SDS-PAGE. Anti-porins antisera were raised in rabbits and specific IgG was purified, which was coupled to Sepharose-CNBr. This immunosorbent was used to purify LPS-free porins.
Pelayo
R
R
Laboratorio de Inmunoquímica, Unidad de Investigación Biomédica, CMN, México, D. F.
Isibasi
A
A
Paniagua
J
J
Ortíz
V
V
Muy
M
M
González
C
C
Blanco
F
F
Islas
S
S
Kumate
J
J
D000907:Antibodies, Bacterial / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology / Q000302:isolation & purification*; D002847:Chromatography, Agarose; D004591:Electrophoresis, Polyacrylamide Gel; D007074:Immunoglobulin G / Q000276:immunology*; D007163:Immunosorbent Techniques; D018272:Porins; D012485:Salmonella typhi / Q000032:analysis* / Q000276:immunology
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D007074:Immunoglobulin G; D018272:Porins
Elaboración de un inmunoadsorbente para la purificación de porinas de Salmonella typhi 9, 12, Vi: d.