title: Vimentin phosphorylation by p37mos protein kinase in vitro and generation of a 50-kDa cleavage product in v-mos-transformed cells.
abstract: Previous studies have shown that vimentin, an intermediate filament protein, is reduced in amount in cells acutely infected with Moloney mouse sarcoma virus (Mo-MuSV). In this report, we provide evidence for specific alteration of vimentin in Mo-MuSV-transformed cells and demonstrate specific phosphorylation of vimentin by the p37mos protein kinase in vitro. Specificity of the phosphorylation reaction was demonstrated by using viral mos proteins encoded by various isolates of Mo-MuSV and p37mos produced in yeast. A phosphotransfer domain mutant lacking the ability to autophosphorylate p37mos failed to phosphorylate vimentin. Similarly, vimentin was not phosphorylated by the temperature-sensitive P85gag-mos kinase derived from infected cells maintained at the restrictive temperature. In ts110 MuSV-transformed NRK cells, vimentin was phosphorylated at both the permissive and nonpermissive temperatures for transformation. However, at the permissive temperature, an altered form of vimentin (about 50 kDa) with a more basic isoelectric point and lower apparent molecular weight was detected. This 50-kDa product was highly phosphorylated as compared to the bulk of the normal 55-kDa form of vimentin. On the basis of its mobility in two-dimensional gels, the 50-kDa form of vimentin should lack the carboxy terminus. This type of alteration could conceivably modulate the function of vimentin filaments in the transformed cell.
authors.affiliation: University of Texas M.D. Anderson Cancer Center, Department of Molecular Pathology, Houston 77030.
authors.forename: B
authors.initials: B
authors.lastname: Singh
authors.forename: R B
authors.initials: RB
authors.lastname: Arlinghaus
mesh_terms: D000818:Animals; D002461:Cell Line, Transformed; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D015683:Gene Products, gag / Q000378:metabolism; D007526:Isoelectric Point; D008970:Molecular Weight; D008980:Moloney murine sarcoma virus / Q000502:physiology*; D017232:Oncogene Proteins v-mos; D010449:Peptide Mapping; D010766:Phosphorylation; D011233:Precipitin Tests; D011494:Protein Kinases / Q000378:metabolism*; D015682:Retroviridae Proteins, Oncogenic / Q000378:metabolism*; D009053:Sarcoma Viruses, Murine / Q000502:physiology*; D013696:Temperature; D014746:Vimentin / Q000378:metabolism*
chemical_list: D015683:Gene Products, gag; D017232:Oncogene Proteins v-mos; D015682:Retroviridae Proteins, Oncogenic; D014746:Vimentin; D011494:Protein Kinases