A 125-base-pair CEN6 DNA fragment is sufficient for complete meiotic and mitotic centromere functions in Saccharomyces cerevisiae.
Saccharomyces cerevisiae centromeres contain a conserved region ranging from 111 to 119 base pairs (bp) in length, which is characterized by the three conserved DNA elements CDEI, CDEII, and CDEIII. We isolated a 125-bp CEN6 DNA fragment (named ML CEN6) containing only these conserved elements and assayed it completely separated from its chromosomal context on circular minichromosomes and on a large linear chromosome fragment. The results show that this 125-bp CEN6 DNA fragment is by itself sufficient for complete mitotic and meiotic centromere functions.
Cottarel
G
G
Institut fuer Mikrobiologie und Molekularbiologie, Justus Liebig Universitaet, Giessen, Federal Republic of Germany.
Shero
J H
JH
Hieter
P
P
Hegemann
J H
JH
D001483:Base Sequence; D002503:Centromere / Q000502:physiology*; D002875:Chromosomes / Q000502:physiology*; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004271:DNA, Fungal / Q000502:physiology*; D008540:Meiosis; D008938:Mitosis; D008969:Molecular Sequence Data; D009630:Nondisjunction, Genetic; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics*
D004271:DNA, Fungal; D004262:DNA Restriction Enzymes