Stimulation of phosphoinositide hydrolysis via class I antigen-specific recognition in murine cardiac tissue.
Induction of polyphosphoinositide hydrolysis in cardiac tissue by specific recognition of class I histocompatibility antigens was assayed. C3H (H-2k) mice auricles were labelled with myo-[3H]inositol precursor and inositol phosphate production in the presence or absence of anti-class I k products was measured. Anti-class I, but not anti-class II products specifically increased phosphoinositide turnover. This increment was partially blocked by muscarinic cholinergic and alpha-adrenergic blockers and even more so by the phospholipase C inhibitor NCDC. Alloantibodies specifically directed against class I antigens could then exert stimulation of phospholipase C-mediated phosphoinositide hydrolysis through the interaction with muscarinic cholinergic and/or alpha-adrenergic receptors. The induction of intracellular second messengers by class I antigens and hormone-receptor interactions is discussed.
Cremaschi
G A
GA
Centro de Estudios Farmacológicos y de Principios Naturales, CONICET, Buenos Aires, Argentina.
Sterin-Borda
L
L
D000818:Animals; D006325:Heart Atria / Q000276:immunology / Q000378:metabolism; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D006868:Hydrolysis; D007074:Immunoglobulin G / Q000276:immunology; D066298:In Vitro Techniques; D007518:Isoantibodies / Q000276:immunology; D051379:Mice; D008809:Mice, Inbred C3H; D009206:Myocardium / Q000276:immunology / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000378:metabolism*; D011942:Receptors, Adrenergic, alpha / Q000378:metabolism; D011950:Receptors, Cholinergic / Q000378:metabolism
D015395:Histocompatibility Antigens Class I; D007074:Immunoglobulin G; D007518:Isoantibodies; D010716:Phosphatidylinositols; D011942:Receptors, Adrenergic, alpha; D011950:Receptors, Cholinergic