title: Incorporation of arachidonic, dihomogamma linolenic and eicosapentaenoic acids into cultured V79 cells.
abstract: The uptake and distribution of three common dietary polyunsaturated fatty acids was studied using Chinese hamster lung fibroblasts (V79 cells). Treatment of V79 cells with arachidonic (20:4), eicosapentaenoic (20:5) and dihomogammalinolenic (20.3) acids for 24 hr produced a marked uptake of 20:3 and 20:4, both of which were assimilated to a considerably greater degree than 20:5. All polyunsaturated fatty acids were incorporated primarily into phospholipids; however, there were considerable differences in their distribution into individual phospholipid species. Although 20:4 was incorporated primarily into phosphatidylcholine, 20:3 entered largely into phosphatidylethanolamine and phosphatidylglycerol, and 20:5 was distributed about equally between phosphatidylcholine, phosphatidylethanolamine and phosphatidylinositol. A marked conversion of 20:3 to 20:4 was found after 24 hr and, in several phospholipids, there was as much derived-radiolabeled 20:4 as there was radiolabeled 20:3. There was little evidence of 20:4 and 20:5 metabolism. V79 cells undergo substantial changes in phospholipid fatty acid composition following supplementation with these polyunsaturated fatty acids; however, these fatty acids are assimilated to different degrees and their distribution among cellular phospholipids is distinct, suggesting incorporation via independent mechanisms.
authors.affiliation: Institute for Toxicology, University of Southern California, School of Pharmacy, Los Angeles 90033.
authors.forename: K U
authors.initials: KU
authors.lastname: Weithmann
authors.forename: H
authors.initials: H
authors.lastname: Peterson
authors.forename: A
authors.initials: A
authors.lastname: Sevanian
mesh_terms: D015126:8,11,14-Eicosatrienoic Acid / Q000378:metabolism; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004041:Dietary Fats / Q000378:metabolism*; D015118:Eicosapentaenoic Acid / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D008042:Linolenic Acids / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D010743:Phospholipids / Q000096:biosynthesis; D017962:alpha-Linolenic Acid
chemical_list: D001095:Arachidonic Acids; D004041:Dietary Fats; D005231:Fatty Acids, Unsaturated; D008042:Linolenic Acids; D010743:Phospholipids; D017962:alpha-Linolenic Acid; D016718:Arachidonic Acid; D015118:Eicosapentaenoic Acid; D015126:8,11,14-Eicosatrienoic Acid