Complementary information from isotachophoresis and high-performance liquid chromatography in peptide analysis.
Reversed-phase high-performance liquid chromatography is a valuable analytical technique to support the synthesis, isolation and purification of peptides, as is illustrated by some critical separations. In addition to this technique, capillary isotachophoresis can give useful information on the purity determination of peptides and on the presence of ionic compounds of a non-peptidic nature. With regard to the latter aspect, isotachophoresis proved to be a suitable technique as a check on the effective removal of salts after preparative high-performance liquid chromatography.
Janssen
P S
PS
AKZO Pharma Division, Organon International B.V., Oss, The Netherlands.
van Nispen
J W
JW
van Zeeland
M J
MJ
Melgers
P A
PA
D000324:Adrenocorticotropic Hormone / Q000031:analogs & derivatives / Q000032:analysis; D000818:Animals; D002417:Cattle; D002766:Cholecystokinin / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004586:Electrophoresis / Q000379:methods; D006801:Humans; D007328:Insulin / Q000032:analysis; D010446:Peptide Fragments / Q000032:analysis; D010455:Peptides / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D013552:Swine; D014667:Vasopressins / Q000032:analysis; D001615:beta-Endorphin / Q000032:analysis
D007328:Insulin; D010446:Peptide Fragments; D010455:Peptides; D014667:Vasopressins; C013765:Org 2766; D001615:beta-Endorphin; D000324:Adrenocorticotropic Hormone; D002766:Cholecystokinin