The action of 4-hydroxynonenal on heat shock gene expression in cultured hepatoma cells.
Hep G2 cells do not synthesize heat-shock proteins when incubated with ADP-iron, under conditions that can trigger lipoperoxidation. However, exposure of these cells to added 4-hydroxynonenal (HNE), one of the main products of lipoperoxidation, induces the synthesis of hsp70, the most conserved among heat-shock proteins. HNE acts mainly on transcription: in Hep G2 cells the increase in the steady-state level of hsp70 mRNA is detectable by two different hybridization techniques.
Cajone
F
F
Istituto di Patologia Generale dell Universita degli Studi di Milano, Italy.
Bernelli-Zazzera
A
A
D000244:Adenosine Diphosphate / Q000494:pharmacology / Q000031:analogs & derivatives; D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D015152:Blotting, Northern; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism*; D015342:DNA Probes; D004586:Electrophoresis; D015870:Gene Expression / Q000187:drug effects*; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D015151:Immunoblotting; D007502:Iron Chelating Agents / Q000494:pharmacology; D008113:Liver Neoplasms; D008114:Liver Neoplasms, Experimental / Q000235:genetics / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000096:biosynthesis; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D051381:Rats; D014407:Tumor Cells, Cultured
D000447:Aldehydes; D015342:DNA Probes; D006360:Heat-Shock Proteins; D007502:Iron Chelating Agents; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; C029936:adenosine diphosphate-ferric chelate; D000244:Adenosine Diphosphate; C027576:4-hydroxy-2-nonenal