Conserved function in Nicotiana tabacum of a single Drosophila hsp70 promoter heat shock element when fused to a minimal T-DNA promoter.
To demonstrate the extent of evolutionary conservation in the mechanism of induction of heat shock genes between plants and animals, the minimal sequence from the Drosophila hsp70 promoter sufficient to confer heat shock inducible transcription in tobacco was determined. Segments of the hsp70 promoter were fused to a minimal promoter of the T-DNA indole-3-acetamide hydrolase (iaaH) gene, in a chimaeric gene fusion to a neomycin phosphotransferase (NPT II) reporter gene. Sequences bearing one or more heat shock elements (HSEs) rendered the minimal promoter heat shock inducible, with a 37 bp fragment containing a single complete HSE sufficing. The induced NPT II mRNA peaked during the heat shock period, but the maximal level of NPT II activity was not observed until 4 h later in the recovery phase, showing that the translation of the NPT II mRNA was shifted from the heat shock period of the recovery phase. That similar sequences containing a single HSE of the Drosophila hsp70 promoter could function in both flies and tobacco indicates the high degree of homology between the two heat shock gene induction systems.
Wing
D
D
Max-Planck-Institut für Züchtungsforschung, Köln, Federal Republic of Germany.
Koncz
C
C
Schell
J
J
D000581:Amidohydrolases / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D004330:Drosophila / Q000235:genetics*; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D006360:Heat-Shock Proteins / Q000235:genetics*; D019868:Kanamycin Kinase; D010770:Phosphotransferases / Q000235:genetics; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D012231:Rhizobium / Q000235:genetics; D014026:Nicotiana / Q000235:genetics*; D014158:Transcription, Genetic; D015533:Transcriptional Activation
D004269:DNA, Bacterial; D006360:Heat-Shock Proteins; C036483:T-DNA; D004247:DNA; D010770:Phosphotransferases; D019868:Kanamycin Kinase; D000581:Amidohydrolases; C058772:indole-3-acetamide hydrolase