title: High-speed DNA sequencing: an approach based upon fluorescence detection of single molecules.
abstract: We are developing a laser based technique for the rapid sequencing of large fragments (approximately 40 kb) of DNA based upon the detection of single, fluorescently tagged nucleotides cleaved from a single DNA fragment. We have demonstrated significant progress on several of the important steps of this technique. The projected rate of sequencing is several hundred bases per second which is orders of magnitude faster than existing methods. Once developed, this technology could be utilized by investigators for rapid sequencing of genetic material from virtually any source.
authors.affiliation: Los Alamos National Laboratory, New Mexico 87545.
authors.forename: J H
authors.initials: JH
authors.lastname: Jett
authors.forename: R A
authors.initials: RA
authors.lastname: Keller
authors.forename: J C
authors.initials: JC
authors.lastname: Martin
authors.forename: B L
authors.initials: BL
authors.lastname: Marrone
authors.forename: R K
authors.initials: RK
authors.lastname: Moyzis
authors.forename: R L
authors.initials: RL
authors.lastname: Ratliff
authors.forename: N K
authors.initials: NK
authors.lastname: Seitzinger
authors.forename: E B
authors.initials: EB
authors.lastname: Shera
authors.forename: C C
authors.initials: CC
authors.lastname: Stewart
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000302:isolation & purification; D005092:Exonucleases; D005456:Fluorescent Dyes; D005470:Fluorometry / Q000379:methods; D007834:Lasers; D008722:Methods
chemical_list: D005456:Fluorescent Dyes; D004247:DNA; D005092:Exonucleases