Effects of continuous exposure to 239Pu-alpha rays on Co631 cells.
Exposure of Co631 cells to 239Pu-citrate leads to continuous irradiation and results in increasing cell death which depends on dose and incubation time of the metal. The D37 is calculated to be 0.13 Gy. Plutonium-239 induces the amplification of the integrated SV40 sequences of the Co631 cells. Other cellular genes such as the alpha-actin gene were not amplified, indicating that the SV40-gene amplification is a selective process. Southern blot analysis of genomic DNA shows that not all S40 sequences are equally amplified. Excised sequences of the SV40 genome only appear after an exposure time of 4 days.
Rau
W
W
Kernforschungszentrum Karlsruhe, Institut für Genetik und Toxikologie, F.R.G.
Olinger
H
H
Planas-Bohne
F
F
Taylor
D M
DM
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects*; D002471:Cell Transformation, Neoplastic; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000528:radiation effects; D004279:DNA, Viral / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D011005:Plutonium; D013539:Simian virus 40 / Q000235:genetics
D004279:DNA, Viral; D011005:Plutonium; D004247:DNA