title: Episomal HPV 16 DNA isolated from a cervical carcinoma presents a partial duplication of the early region.
abstract: An invasive cervical carcinoma was found to harbor an episomal variant of human papillomavirus (HPV) type 16 DNA, with a size of about 10.1 kb. A genomic library of the tumor was constructed in bacteriophage lambda and a recombinant phage clone was isolated by screening with HPV 16 probe. Analysis by restriction mapping and Southern hybridization showed that the isolate contained a 2.2 kb duplication of the early region, which included part of E6, all E7 and part of E1 open reading frames. Possible consequences of this duplication for oncogenesis are discussed.
authors.affiliation: Istituto di Microbiologia, Università di Ferrara, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Di Luca
authors.forename: E
authors.initials: E
authors.lastname: Caselli
authors.forename: P
authors.initials: P
authors.lastname: Monini
authors.forename: A
authors.initials: A
authors.lastname: Rotola
authors.forename: A
authors.initials: A
authors.lastname: Savioli
authors.forename: E
authors.initials: E
authors.lastname: Cassai
mesh_terms: D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D015321:Gene Rearrangement; D005814:Genes, Viral; D006801:Humans; D005810:Multigene Family; D027383:Papillomaviridae / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D015183:Restriction Mapping; D002583:Uterine Cervical Neoplasms / Q000382:microbiology*
chemical_list: D015344:DNA Probes, HPV; D004279:DNA, Viral