Expression of interleukin 2 receptors in T cells transformed by strains of Herpesvirus saimiri representing three DNA subgroups.
Eight T lymphoblastoid cell lines transformed by Herpesvirus saimiri were studied for requirement of interleukin 2 (IL-2) for growth in tissue culture and expression of the IL-2 receptor. The results show that four cell lines grew independent of IL-2, but four other cell lines required IL-2 for optimal growth. However, all H. saimiri transformed cells expressed IL-2 receptors, regardless of IL-2 requirement. Between 2,250 and 8,800 high-affinity receptor sites/cell were detected by ligand-binding assays; in contrast, only about 1,000 sites/cell were in an uninfected IL-2-dependent marmoset T cell line. Surface-bound IL-2 was internalized and antibodies to the receptor inhibited cell growth. Expression of IL-2 receptors may allow expansion of tumor cells in vivo in response to endogenous or exogenous IL-2.
Medveczky
P G
PG
Department of Pharmacology, University of Massachusetts Medical School, Worcester 01655.
Medveczky
M M
MM
D000818:Animals; D000906:Antibodies / Q000276:immunology; D015152:Blotting, Northern; D002455:Cell Division; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D005455:Fluorescent Antibody Technique; D006567:Herpesvirus 2, Saimiriine / Q000502:physiology*; D007376:Interleukin-2 / Q000378:metabolism / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D015375:Receptors, Interleukin-2 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000378:metabolism* / Q000382:microbiology
D000906:Antibodies; D007376:Interleukin-2; D012333:RNA, Messenger; D012367:RNA, Viral; D015375:Receptors, Interleukin-2; D011994:Recombinant Proteins