Radiation nephropathy in mature pigs following the irradiation of both kidneys.
Both kidneys in mature female Large White pigs, ca. 45 weeks old were irradiated with single doses of 7.8-14.0 Gy of 60Co gamma-rays. Radiation-induced changes in renal function were assessed on the basis of sequential individual measurements of kidney glomerular filtration rate (GFR) and effective renal plasma flow (ERPF) for up to 24 weeks after irradiation. At the same time intervals blood samples were taken to determine plasma renin levels and the haematological status of each animal. Two weeks after irradiation the ERPF and in particular the GFR was increased. This was followed by a pronounced, dose-dependent, decline in renal haemodynamics. Minimal functional levels were observed 8-12 weeks after irradiation. Function then somewhat recovered. There was a significant (r greater than or equal to 0.98; p less than 0.001) inverse relationship between the mean values of GFR and ERPF, determined at 4-24 weeks after irradiation, and the radiation dose. After a dose of 7.8 Gy the reduction in ERPF was greater than that for GFR. However, at higher doses both parameters were reduced to an equal extent. The resulting slope of the dose-effect curve for impaired renal function versus dose was significantly steeper for GFR than that for ERPF (p less than 0.001). There was a significant reduction (p less than 0.002) in the erythrocyte count, the haematocrit and haemoglobin levels within 6-8 weeks of irradiation; this anaemia was characterized as a normochromic normocytic anaemia. There were no marked changes in plasma renin levels as a result of renal irradiation. The pathogenesis of late radiation-induced damage to the kidney is discussed in the light of these findings.
Robbins
M E
ME
CRC Normal Tissue Radiobiology Research Group, Research Institute (University of Oxford), Churchill Hospital, U.K.
Campling
D
D
Rezvani
M
M
Golding
S J
SJ
Hopewell
J W
JW
D000818:Animals; D003037:Cobalt Radioisotopes; D005260:Female; D005720:Gamma Rays; D005919:Glomerular Filtration Rate / Q000528:radiation effects; D007668:Kidney / Q000528:radiation effects*; D007674:Kidney Diseases / Q000209:etiology* / Q000503:physiopathology; D012079:Renal Circulation / Q000528:radiation effects; D012083:Renin / Q000097:blood; D013552:Swine
D003037:Cobalt Radioisotopes; D012083:Renin