Vasoconstrictor-induced heterologous down-regulation of vascular atrial natriuretic peptide receptor.
Long-term (24 h) pretreatment of cultured rat vascular smooth muscle cells with 100 nM angiotensin and 1 microM vasopressin induced a marked reduction of the maximal binding capacity of atrial natriuretic peptide (ANP) receptors in a fashion similar to that induced by phorbol ester. The down-regulation of the receptors induced by vasoconstrictors and phorbol ester was concomitantly associated with an attenuation of ANP-stimulated cGMP accumulation. These data suggest that vasoconstrictor-induced activation of protein kinase C is involved in the mechanism of heterologous down-regulation of vascular ANP receptors.
Hirata
Y
Y
Second Department of Internal Medicine, Tokyo Medical and Dental University, Japan.
Emori
T
T
Ohta
K
K
Shichiri
M
M
Marumo
F
F
D000804:Angiotensin II / Q000502:physiology; D000818:Animals; D001127:Arginine Vasopressin / Q000502:physiology; D006152:Cyclic GMP / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism; D010880:Piperidines / Q000378:metabolism; D051381:Rats; D017461:Receptors, Atrial Natriuretic Factor; D011956:Receptors, Cell Surface / Q000502:physiology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014662:Vasoconstrictor Agents / Q000494:pharmacology*
D007457:Iodine Radioisotopes; D010880:Piperidines; D011956:Receptors, Cell Surface; D014662:Vasoconstrictor Agents; D000804:Angiotensin II; D001127:Arginine Vasopressin; C026539:preclamol; D017461:Receptors, Atrial Natriuretic Factor; D006152:Cyclic GMP; D013755:Tetradecanoylphorbol Acetate