title: PAF-acether, superoxide anion and beta-glucuronidase as parameters of polymorphonuclear cell activation associated with cardiac surgery and cardiopulmonary bypass.
abstract: 1. Seven patients submitted to myocardial revascularization surgery with cardiopulmonary bypass were studied. Blood samples were obtained immediately before and 24 h after surgery. The parameters studied were the production of platelet activating factor (PAF-acether) and superoxide anion, cellular beta-glucuronidase activity as well as polymorphonuclear cell (PMN) and platelet counts. 2. Twenty-four h after surgery, there was a 54% decrease in platelet number (P less than 0.005), a 121% increase in PMN number (P less than 0.005), a 353% increase in PAF-acether (P less than 0.01), a 211% increase in superoxide anion (O2-) and a 104% increase in beta-glucuronidase (P less than 0.05) levels when compared with the pre-surgery levels. 3. The present results indicate that PMN are more reactive after surgery with cardiopulmonary bypass.
authors.affiliation: Instituto do Coração, Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo, Brasil.
authors.forename: A Y
authors.initials: AY
authors.lastname: Hoshikawa-Fujimura
authors.forename: J O
authors.initials: JO
authors.lastname: Auler Júnior
authors.forename: T R
authors.initials: TR
authors.lastname: Da Rocha
authors.forename: L I
authors.initials: LI
authors.lastname: Brandizzi
authors.forename: J M
authors.initials: JM
authors.lastname: Pascual
authors.forename: D A
authors.initials: DA
authors.lastname: Chamone
authors.forename: A D
authors.initials: AD
authors.lastname: Jatene
mesh_terms: D001772:Blood Cell Count; D002315:Cardiopulmonary Bypass; D005966:Glucuronidase / Q000097:blood*; D006801:Humans; D008875:Middle Aged; D009204:Myocardial Revascularization; D009504:Neutrophils / Q000502:physiology*; D010972:Platelet Activating Factor / Q000096:biosynthesis*; D010976:Platelet Count; D013481:Superoxides / Q000097:blood*
chemical_list: D010972:Platelet Activating Factor; D013481:Superoxides; D005966:Glucuronidase