Production of salivary type alpha-amylase in human lung cancer.
alpha-Amylase, which is produced by lung cancer tissue, was studied by cloning cDNAs from a cell line originating from lung cancer that produces amylase. Sequencing studies with this cDNA showed that the expressing gene is of the salivary type. The specific location of the start point of transcription, as revealed by S1 mapping, supported this conclusion.
Nakamura
Y
Y
Institute for Molecular and Cellular Biology, Osaka University, Suita, Japan.
Tomita
N
N
Nishide
T
T
Emi
M
M
Horii
A
A
Ogawa
M
M
Mori
T
T
Kosaki
G
G
Okabe
T
T
Fujisawa
M
M
D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000235:genetics; D004720:Endonucleases; D005091:Exons; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics*; D012334:RNA, Neoplasm / Q000096:biosynthesis / Q000235:genetics*; D015183:Restriction Mapping; D012471:Salivary Proteins and Peptides / Q000096:biosynthesis / Q000235:genetics; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014407:Tumor Cells, Cultured; D000516:alpha-Amylases / Q000096:biosynthesis* / Q000235:genetics
D015342:DNA Probes; D004273:DNA, Neoplasm; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D012471:Salivary Proteins and Peptides; D004247:DNA; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases; D000516:alpha-Amylases