title: Electrogenic sodium-proton exchange in membrane vesicles from crab (Carcinus maenas) gill.
abstract: Plasma membrane vesicles from Na+-absorbing posterior gills of the green crab Carcinus maenas were shown to contain a Na+-H+ antiporter sensitive to amiloride (Ki = 280 mumol/l), using a fluorometric assay based on acridine orange. Lithium was found to support Li+-H+ exchange, giving Michaelis-Menten kinetics (K0.5 = 20 mmol/l) and a Hill coefficient of 1.32 +/- 0.06, indicating a stoichiometry of 1 Li+:1 H+. The stoichiometry of Na+-H+ exchange, however, appeared to be 2 Na+:1 H+. The dependence of exchange rate on Na+ concentration followed a sigmoid relationship (K0.5 = 34 mmol/l), producing a Hill coefficient of 1.99 +/- 0.18. Measurements made with the potential-sensitive dye 3,3'-dipropylthiadicarboxocyanine iodide directly demonstrated electrogenicity of Na+-H+ exchange. Na+-loaded vesicles showed greater potential change upon addition to Na+-free medium than upon addition to Na+-containing medium, and the developed potential was reversed by the addition of external Na+. Amiloride reduced the rate of membrane polarization and abolished the reversal by external Na+. The crustacean Na+-H+ antiporter therefore bears similarities to the vertebrate antiporter but is uniquely electrogenic.
authors.affiliation: Mount Desert Island Biological Laboratory, Salsbury Cove, Maine 04672.
authors.forename: R E
authors.initials: RE
authors.lastname: Shetlar
authors.forename: D W
authors.initials: DW
authors.lastname: Towle
mesh_terms: D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D003386:Brachyura; D002352:Carrier Proteins / Q000378:metabolism*; D002458:Cell Fractionation; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D003576:Electron Transport Complex IV / Q000378:metabolism; D005880:Gills / Q000502:physiology*; D007700:Kinetics; D008094:Lithium / Q000494:pharmacology; D008564:Membrane Potentials; D012964:Sodium / Q000378:metabolism; D017923:Sodium-Hydrogen Exchangers; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D017923:Sodium-Hydrogen Exchangers; D000584:Amiloride; D008094:Lithium; D012964:Sodium; D003576:Electron Transport Complex IV; D000254:Sodium-Potassium-Exchanging ATPase