Mechanism of the reversal reaction of platinated DNA with thiourea studied by platinated 5'-GMP.
The reversal reactions of cis-[Pt(NH3)2(5'-GMP)2] 2-(1) and trans-[Pt(NH3)2(5'-GMP)2] 2-(2) with thiourea were examined by reversed phase HPLC and monothioureido intermediate cis-[Pt(NH3)2(5'-GMP) (tu)] (4) was detected. This result suggested that Pt-[5'-GMP-N(7)] bond was more labile than Pt-NH3 bond and the release of ammonia from cis-Pt(II)-DNA base complexes is a result of trans-labilizing effect of sulfur containing molecule displaced with DNA base.
Urata
H
H
Osaka University of Pharmaceutical Sciences, Japan.
Akagi
M
M
D000641:Ammonia; D002851:Chromatography, High Pressure Liquid; D002945:Cisplatin / Q000037:antagonists & inhibitors*; D004249:DNA Damage; D006150:Guanine Nucleotides / Q000031:analogs & derivatives*; D006157:Guanosine Monophosphate / Q000031:analogs & derivatives*; D007700:Kinetics; D008956:Models, Chemical; D013890:Thiourea / Q000494:pharmacology*
D006150:Guanine Nucleotides; D000641:Ammonia; D006157:Guanosine Monophosphate; D013890:Thiourea; D002945:Cisplatin