Replication of the genome RNAs of defective interfering particles of vesicular stomatitis and Sendai viruses using heterologous viral proteins.
We have tested the ability of heterologous viral proteins to support the in vivo and in vitro replication of the RNA of defective interfering (DI) particles of two serotypes of VSV and of Sendai virus. In all the combinations of heterologous coinfections in vivo, DI particle replication was observed only in the coinfection with the VSV-Indiana DI particle and wild-type VSV-New Jersey. By quantitating RNA synthesis in reconstitution experiments we showed that with DI nucleocapsids isolated from infected cells, however, the soluble protein fraction from heterologous wild-type virus-infected cells could substitute in vitro to varying degrees for the homologous proteins in the elongation reaction of RNA replication and encapsidation. In these cases successful replication was confirmed by demonstrating the specific association of the heterologous N protein with the product nucleocapsid RNA. The initiation step, that is, the initial binding of the nucleocapsid protein to the leader RNA, in contrast, requires the homologous protein, since heterologous viral proteins could not support RNA replication and encapsidation from purified DI particles.
Moyer
S A
SA
Department of Biochemistry and Molecular Biology, University of Florida College of Medicine, Gainesville 32610.
D002213:Capsid / Q000502:physiology; D003673:Defective Viruses / Q000235:genetics*; D009024:Morphogenesis; D010222:Parainfluenza Virus 1, Human / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D013045:Species Specificity; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics*; D014758:Viral Core Proteins / Q000502:physiology; D014762:Viral Interference; D014764:Viral Proteins / Q000494:pharmacology*; D014779:Virus Replication
D012367:RNA, Viral; D014758:Viral Core Proteins; D014764:Viral Proteins