Antibody 624H12, which detects lung cancer at early stages, recognizes a sugar sequence in the glycosphingolipid difucosylneolactonorhexaosylceramide (V3FucIII3FunLc6Cer).
Immunocytochemical staining of cells in sputum by rat monoclonal antibody 624H12 detects lung cancer 2 years prior to its detection by conventional diagnostic techniques. The antigen recognized by antibody 624H12 is a sugar sequence in the glycosphingolipid difucosylneolactonorhexaosylceramide (V3FucIII3FucnLc6Cer) whose structure is (formula see; text) Both fucosyl residues are required for high affinity binding by the antibody. The antigen was expressed in 35 of 45 specimens of cancer tissue from patients with early stage non small cell lung cancer. There was no correlation between antigen expression and patient survival.
Kyogashima
M
M
Laboratory of Structural Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892.
Mulshine
J
J
Linnoila
R I
RI
Jensen
S
S
Magnani
J L
JL
Nudelman
E
E
Hakomori
S
S
Ginsburg
V
V
D000230:Adenocarcinoma / Q000175:diagnosis; D000818:Animals; D000911:Antibodies, Monoclonal; D015415:Biomarkers / Q000032:analysis*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D018288:Carcinoma, Small Cell / Q000175:diagnosis; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis; D006028:Glycosphingolipids / Q000032:analysis* / Q000276:immunology; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis*; D008969:Molecular Sequence Data; D051381:Rats; D013183:Sputum / Q000032:analysis*
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D006028:Glycosphingolipids; C061560:difucosylneolactonorhexaosylceramide