title: Explants of human oral epithelium exposed to viruses and cancer chemotherapeutics.
abstract: Cultures of proliferating epithelial cells were established from explants of normal human oral epithelium from healthy young volunteers. The epithelial cells were found permissive for herpes simplex virus type 1 and type 2, coxsackie virus A-4 and A-16, adenovirus type 5, measles vaccine, rubella and influenza type A virus-. Medium from DEAE-pretreated epithelial cultures infected with two subtypes of human immunodeficiency virus-1 showed an increasing content of virusprotein with time by antigen ELISA testing. In contrast there was no evidence of infection with coxsackie virus type B-2, cytomegalovirus, Epstein-Barr virus and varicella zoster virus. Treatment of the epithelial cells with a non-cytotoxic dose of cancer chemotherapeutic prior to or after infection with coxsackie virus A-4 or herpes simplex virus type 1 influenced the virus production dependent on both compound, mode of application, and virus. Adriamycin (doxorubicin) in low dose was found to stimulate the production of the two viruses.
authors.affiliation: Danish Cancer Society, Department of Virus and Cancer, Aarhus.
authors.forename: P
authors.initials: P
authors.lastname: Ebbesen
authors.forename: P M
authors.initials: PM
authors.lastname: Petersen
authors.forename: A
authors.initials: A
authors.lastname: Jepsen
authors.forename: N
authors.initials: N
authors.lastname: Nørskov-Lauritsen
authors.forename: C M
authors.initials: CM
authors.lastname: Nielsen
authors.forename: H P
authors.initials: HP
authors.lastname: Philipsen
authors.forename: D
authors.initials: D
authors.lastname: Arenholt-Bindslev
authors.forename: P
authors.initials: P
authors.lastname: Nara
mesh_terms: D000328:Adult; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002166:Camptothecin / Q000494:pharmacology; D002478:Cells, Cultured; D004267:DNA Viruses / Q000187:drug effects / Q000502:physiology*; D004317:Doxorubicin / Q000494:pharmacology; D004770:Enterovirus / Q000187:drug effects / Q000502:physiology; D004848:Epithelium / Q000187:drug effects / Q000382:microbiology; D015497:HIV-1 / Q000187:drug effects / Q000502:physiology; D006801:Humans; D009061:Mouth Mucosa / Q000187:drug effects / Q000382:microbiology*; D012328:RNA Viruses / Q000187:drug effects / Q000502:physiology*; D018139:Simplexvirus / Q000187:drug effects / Q000502:physiology; D014750:Vincristine / Q000494:pharmacology; D014777:Virus Diseases
chemical_list: D000970:Antineoplastic Agents; D014750:Vincristine; D004317:Doxorubicin; D002166:Camptothecin