title: Effect of high NaCl intake on Na+ and K+ transport in the rabbit distal convoluted tubule.
abstract: Morphological studies have demonstrated that a chronic increase in distal Na+ delivery causes hypertrophy of the distal convoluted tubule (DCT). To examine whether high NaCl-intake also causes functional changes in the well defined DCT, we measured transmural voltage (VT), lumen-to-bath Na+ flux (JNa(LB], and net K+ secretion (JK(net] in DCTs obtained from control rabbits and those on high NaCl-intake diets. The lumen negative VT was significantly greater in the high NaCl group than in the control group. The net K+ secretion (pmol mm-1 min-1) was greater in the high NaCl-intake group (54.1 +/- 13.0 vs 14.7 +/- 5.6). The K+ permeabilities in both luminal and basolateral DCT membranes, as assessed by the K+-induced transepithelial voltage deflection inhibitable with Ba2+, were increased in the experimental group. The lumen-to-bath 22Na flux (pmol mm-1 min-1) was also greater in the experimental group (726 +/- 119 vs 396 +/- 65). The VT component inhibitable with amiloride was also elevated in the high NaCl-intake group. Furthermore, Na+-K+-ATPase activity of the DCT was higher in the experimental than in the control group. We conclude that high NaCl intake increases both Na+ reabsorption and K+ secretion by the DCT. This phenomenon is associated with an increased Na+-K+-ATPase activity along with increased Na+ and K+ permeabilities of the luminal membrane, and an increase in the K+ permeability of the basolateral membrane. Cellular mechanisms underlying these functional changes remain to be established.
authors.affiliation: Department of Pharmacology, National Cardiovascular Center, Osaka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Shimizu
authors.forename: K
authors.initials: K
authors.lastname: Yoshitomi
authors.forename: J
authors.initials: J
authors.lastname: Taniguchi
authors.forename: M
authors.initials: M
authors.lastname: Imai
mesh_terms: D000450:Aldosterone / Q000097:blood; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D066298:In Vitro Techniques; D007684:Kidney Tubules / Q000187:drug effects*; D007686:Kidney Tubules, Distal / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D011188:Potassium / Q000378:metabolism*; D011817:Rabbits; D012083:Renin / Q000097:blood; D012964:Sodium / Q000378:metabolism*; D012965:Sodium Chloride / Q000008:administration & dosage*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
chemical_list: D012965:Sodium Chloride; D000450:Aldosterone; D012964:Sodium; D012083:Renin; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium