title: Beta-adrenergic and opioid receptors on pituicytes cultured from adult rat neurohypophysis: regulation of cell morphology.
abstract: Explants of adult rat neurohypophysis were maintained in culture for 14 days. The majority of cells present in the outgrowth of such cultures were identified as pituicytes on the basis of immunostaining for glial fibrillary acidic protein. Pituicytes were also stained by antisera to the membrane glycoprotein antigen Thy-1 and the extracellular matrix glycoprotein fibronectin. The cultures contained naloxone sensitive binding sites for the opioid receptor ligand [3H] dynorphin A 1-8 and peripheral-type benzodiazepine binding sites. Dynorphin binding was visualised over pituicytes following autoradiography. The morphology of cultured pituicytes was regulated by beta-adrenergic receptors present on the cells which, when activated, stimulated rapid transformation from a flattened irregular morphology to a stellate, process-bearing morphology. Dynorphin was without effect on the morphology of cultured pituicytes. These findings are discussed in the context of the known morphological plasticity of pituicytes in vivo.
authors.affiliation: Department of Neuroendocrinology, AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, UK.
authors.forename: R J
authors.initials: RJ
authors.lastname: Bicknell
authors.forename: S M
authors.initials: SM
authors.lastname: Luckman
authors.forename: K
authors.initials: K
authors.lastname: Inenaga
authors.forename: W T
authors.initials: WT
authors.lastname: Mason
authors.forename: G I
authors.initials: GI
authors.lastname: Hatton
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism; D002478:Cells, Cultured; D004399:Dynorphins / Q000378:metabolism*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D008297:Male; D009270:Naloxone / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism*; D010904:Pituitary Gland, Posterior / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D011957:Receptors, Opioid / Q000378:metabolism*; D018800:Thy-1 Antigens
chemical_list: D000954:Antigens, Surface; D005904:Glial Fibrillary Acidic Protein; D010446:Peptide Fragments; D011943:Receptors, Adrenergic, beta; D011957:Receptors, Opioid; D018800:Thy-1 Antigens; D009270:Naloxone; D004399:Dynorphins; C026770:dynorphin (1-8)