Inhibition of in vitro tumor cell invasion by transmethylation inhibitors.
Three inhibitors of S-adenosylmethionine-mediated transmethylation, 5'-methylthioadenosine (MTA), 2'-deoxyadenosine and sinefungin, inhibited in vitro invasion by a highly invasive clone (Cl-30) of rat ascites hepatoma cells, AH 130 (AH cells). Difluoromethylthioadenosine (DFMTA), a non-metabolizable derivative of MTA, also caused strong inhibition of invasion at concentrations that did not suppress the growth of the tumor cells. Cl-30 cells precultured in methionine-depleted medium showed decreased invasiveness. DFMTA was also effective on the invasion by fibrosarcoma, B16 melanoma and human lung carcinoma cell lines.
Shinkai
K
K
Department of Tumor Biochemistry, Research Institute, Center for Adult Diseases, Osaka.
Mukai
M
M
Horai
T
T
Ohigashi
H
H
Nishikawa
S
S
Inoue
H
H
Takeda
Y
Y
Akedo
H
H
D000241:Adenosine / Q000494:pharmacology / Q000031:analogs & derivatives; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D018288:Carcinoma, Small Cell / Q000473:pathology*; D003839:Deoxyadenosines / Q000494:pharmacology*; D005354:Fibrosarcoma / Q000473:pathology*; D006801:Humans; D007700:Kinetics; D008114:Liver Neoplasms, Experimental / Q000473:pathology*; D008175:Lung Neoplasms / Q000473:pathology*; D008546:Melanoma, Experimental / Q000473:pathology*; D008745:Methylation; D009361:Neoplasm Invasiveness; D051381:Rats; D011919:Rats, Inbred Strains; D013872:Thionucleosides / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects*
D000970:Antineoplastic Agents; D003839:Deoxyadenosines; D013872:Thionucleosides; C054301:5'-difluoromethylthioadenosine; C008500:5'-methylthioadenosine; D000241:Adenosine; C058118:2'-deoxyadenosine; C006235:sinefungin