Antibody response to recombinant lambda gt11 fusion proteins in cytomegalovirus infection.
Significant epitopes of two of the major cytomegalovirus antigens, a nonstructural DNA-binding protein of 52 kilodaltons (kDa) and a structural phosphoprotein of 150 kDa, expressed as fusion proteins with the beta-galactosidase, were induced in Escherichia coli after infection with recombinant lambda gt11 clones. The epitopes were then used in immunoblotting to assay specific immunoglobulin G (IgG) and IgM in several groups of sera from long-term seropositive subjects and from patients undergoing primary or secondary virus infection. The data obtained showed that IgM reacting with the 52-kDa nonstructural antigen are linked to primary virus infection and can therefore be considered a serological marker of this infection.
Landini
M P
MP
Medical Faculty, Institute of Microbiology, St. Orsola General Hospital, Bologna, Italy.
Lazzarotto
T
T
Ripalti
A
A
Guan
M X
MX
La Placa
M
M
D000914:Antibodies, Viral / Q000096:biosynthesis*; D000956:Antigens, Viral; D003586:Cytomegalovirus Infections / Q000276:immunology*; D004268:DNA-Binding Proteins / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D008970:Molecular Weight; D010750:Phosphoproteins / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D004268:DNA-Binding Proteins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D010750:Phosphoproteins; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins