title: Effects of Aroclor 1254 on dopamine and norepinephrine concentrations in pheochromocytoma (PC-12) cells.
abstract: Pheochromocytoma (PC-12) cells synthesize, store, release and metabolize dopamine (DA) and norepinephrine (NE) in a manner analogous to that observed in the mammalian central nervous system. These cells were used to develop and validate an alternate method to animal testing to assess the effects of a complex environmental mixture of polychlorinated biphenyls (Aroclor 1254) on cellular catecholamine function. Aroclor 1254, at concentrations of 1 to 100 ppm, significantly decreased cellular catecholamine concentrations after 6 hrs. Exposure at 100 ppm for periods of less than an hr increased cellular catecholamine concentrations while longer exposure times (i.e., 1 to 24 hr) decreased cellular catecholamine concentrations. This in vitro depletion of catecholamines is similar to that seen in vivo. Thus, PC-12 cells may be useful for neurochemical evaluation of neurotoxicants with particular reference to effects on catecholaminergic systems.
authors.affiliation: Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509.
authors.forename: R F
authors.initials: RF
authors.lastname: Seegal
authors.forename: K
authors.initials: K
authors.lastname: Brosch
authors.forename: B
authors.initials: B
authors.lastname: Bush
authors.forename: M
authors.initials: M
authors.lastname: Ritz
authors.forename: W
authors.initials: W
authors.lastname: Shain
mesh_terms: D000310:Adrenal Gland Neoplasms / Q000378:metabolism*; D000311:Adrenal Glands / Q000473:pathology; D000818:Animals; D001140:Aroclors / Q000633:toxicity*; D002250:Carbon Radioisotopes; D002395:Catecholamines / Q000378:metabolism; D020111:Chlorodiphenyl (54% Chlorine); D003470:Culture Media; D003847:Deoxyglucose / Q000493:pharmacokinetics; D004298:Dopamine / Q000378:metabolism*; D007424:Intracellular Fluid / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism*; D010673:Pheochromocytoma / Q000378:metabolism* / Q000473:pathology; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D051381:Rats; D014407:Tumor Cells, Cultured
chemical_list: D001140:Aroclors; D002250:Carbon Radioisotopes; D002395:Catecholamines; D003470:Culture Media; D020111:Chlorodiphenyl (54% Chlorine); D003847:Deoxyglucose; D011078:Polychlorinated Biphenyls; D004298:Dopamine; D009638:Norepinephrine