A murine monoclonal antibody that recognizes an extracellular domain of the human c-erbB-2 protooncogene product.
A murine IgM monoclonal antibody, designated SV2-61, was generated against human c-erbB-2 gene-transfected NIH-3T3 (SV11) cells. SV2-61 defined a 185-kDa molecule present on the surface of SV11 cells, another line of c-erbB-2 gene-transfected NIH-3T3 (A4-15) cells, and MKN-7 human gastric cancer cell line carrying an amplified human c-erbB-2 gene. The SV2-61-defined antigen was found to show protein kinase activity in vitro. The SV2-61 was reactive with human c-erbB-2 gene-transfected NIH-3T3 cell lines but not with transfectants carrying c-erbB-2 gene mutants which lack a coding region for the extracellular domain. It was reactive with a portion of human epithelial cell lines but not with native NIH-3T3, TGF-alpha-coding gene-, activated c-raf gene- or Ha-ras gene-transfected NIH-3T3 cells, or non-epithelial human cells. These results indicate that the SV2-61 is an antibody which recognizes an extracellular domain of the c-erbB-2 gene product, 185-kDa protein.
Masuko
T
T
Department of Hygienic Chemistry, Tohoku University, Sendai.
Sugahara
K
K
Kozono
M
M
Otsuki
S
S
Akiyama
T
T
Yamamoto
T
T
Toyoshima
K
K
Hashimoto
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D066246:ErbB Receptors / Q000032:analysis; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D010766:Phosphorylation; D011518:Proto-Oncogene Proteins / Q000032:analysis* / Q000276:immunology; D018719:Erb-b2 Receptor Tyrosine Kinases; D014162:Transfection; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D066246:ErbB Receptors; D011518:Proto-Oncogene Proteins; D018719:Erb-b2 Receptor Tyrosine Kinases