DNA transfection into mammalian cells, selective markers and gene cointegration.
The dominant selective markers that we and others have constructed for the selection of cells which stably acquired a foreign DNA, are briefly described. The possibility of obtaining the cointegration of several genes, and of inducing their amplification in the recipient cell, is discussed.
Colbère-Garapin
F
F
Unité de Virologie Médicale, Institut Pasteur, Paris, France.
Ryhiner
M L
ML
Garapin
A C
AC
D000243:Adenosine Deaminase / Q000235:genetics; D000429:Alcohol Oxidoreductases / Q000235:genetics; D001221:Aspartate Carbamoyltransferase / Q000235:genetics; D002223:Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) / Q000235:genetics; D002478:Cells, Cultured; D004080:Dihydroorotase / Q000235:genetics; D005819:Genetic Markers; D019868:Kanamycin Kinase; D009097:Multienzyme Complexes / Q000235:genetics; D010430:Pentosyltransferases / Q000235:genetics; D010770:Phosphotransferases / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics; D014162:Transfection / Q000379:methods*
C033626:CAD trifunctional enzyme; D005819:Genetic Markers; D009097:Multienzyme Complexes; D011994:Recombinant Proteins; D000429:Alcohol Oxidoreductases; C021358:histidinol dehydrogenase; D013762:Tetrahydrofolate Dehydrogenase; D001221:Aspartate Carbamoyltransferase; D010430:Pentosyltransferases; C024827:xanthine phosphoribosyltransferase; D010770:Phosphotransferases; D019868:Kanamycin Kinase; D004080:Dihydroorotase; D000243:Adenosine Deaminase; D002223:Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)