title: Immunoaffinity isolation of Na,K-ATPase alpha 3 isoform from pig kidney.
abstract: The Na,K-ATPase alpha 3 isoform of the catalytic subunit has been isolated from pig kidney microsomes. The procedure employs immunoaffinity chromatography on Sepharose 4B covalently coupled with monospecific antibodies a-II against the synthetic peptide including the putative alpha 3 N-terminus. The structural analysis provides unambiguous proof that the isolated protein corresponds to the third transcript for the alpha 3 isoform. The N-terminal amino acid sequence determined. Met-Gly-Asp-Lys-Lys-Asp-Asp, shows that unlike the alpha 1 and alpha 2 proteins, the mature Na,K-ATPase isoform lacks post-translational proteolytic processing.
authors.affiliation: Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, Moscow.
authors.forename: E A
authors.initials: EA
authors.lastname: Arystarkhova
authors.forename: O E
authors.initials: OE
authors.lastname: Lakhtina
authors.forename: N B
authors.initials: NB
authors.lastname: Levina
authors.forename: N N
authors.initials: NN
authors.lastname: Modyanov
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D007527:Isoenzymes / Q000302:isolation & purification*; D007668:Kidney / Q000201:enzymology*; D008861:Microsomes / Q000201:enzymology; D008969:Molecular Sequence Data; D000254:Sodium-Potassium-Exchanging ATPase / Q000302:isolation & purification*; D013552:Swine
chemical_list: D000906:Antibodies; D000936:Antigen-Antibody Complex; D007527:Isoenzymes; D000254:Sodium-Potassium-Exchanging ATPase