Characterization of simian immunodeficiency virus-specific T-cell-mediated cytotoxic response of infected rhesus macaques.
Four juvenile rhesus macaques were infected with simian immunodeficiency virus (SIV)MAC-Freshly isolated peripheral blood mononuclear cells (PBMC) from these SIVMAC-infected and from uninfected control macaques were assessed for cytotoxic T-lymphocyte (CTL) activity monthly for 7 consecutive months, beginning 2 months after infection. Target cells consisted of major histocompatibility complex (MHC) haploidentical parental PBMC which were stimulated with mitogen and then pulsed with heat-killed SIVMAC. CTL activity was demonstrated on all four infected animals. The effector cells are T cells which mediate cytotoxicity against SIVMAC-pulsed target cells in an MHC-restricted manner. Furthermore, the cytotoxicity is virus specific and predominantly, if not exclusively, mediated by CD8+ T cells; it is also MHC class-I restricted. Incubation of target cells with leupeptin prior to the cytotoxic assay inhibited target cell generation, suggesting that viral antigens are processed via an endocytic pathway.
Vowels
B R
BR
Department of Pathology, University of California, Davis 95616.
Gershwin
M E
ME
Gardner
M B
MB
Ahmed-Ansari
A
A
McGraw
T P
TP
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D015153:Blotting, Western; D004195:Disease Models, Animal; D005260:Female; D007694:Killer Cells, Natural / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D007976:Leupeptins / Q000494:pharmacology; D008253:Macaca mulatta; D008285:Major Histocompatibility Complex; D008297:Male; D010641:Phenotype; D012192:Retroviridae Infections / Q000276:immunology*; D012397:Rosette Formation; D015302:Simian Immunodeficiency Virus / Q000276:immunology*; D013045:Species Specificity; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000914:Antibodies, Viral; D007976:Leupeptins