Site-directed transcription initiation with a mobile promoter.
A method is described for the high-level transcription of any DNA segment using bacteriophage RNA polymerases (RNAPs). A synthetic mobile promoter with a template-complementary 3' extension is ligated to the target sequence of interest. Transcription with an appropriate RNAP results in an amplification of approx. 70-fold. In the presence of heterologous DNA, bacteriophage RNAPs are shown to be specific for their cognate mobile promoters.
Loewy
Z G
ZG
Lifecodes Corporation, Valhalla, NY 10595.
Leary
S L
SL
Baum
H J
HJ
D001435:Bacteriophages / Q000201:enzymology*; D001483:Base Sequence; D004251:DNA Transposable Elements; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D012486:Salmonella typhimurium / Q000201:enzymology; D013604:T-Phages / Q000201:enzymology; D013698:Templates, Genetic; D014158:Transcription, Genetic
D004251:DNA Transposable Elements; D015345:Oligonucleotide Probes; D012321:DNA-Directed RNA Polymerases