Increased expression of sialyl-dimeric LeX antigen in liver metastases of human colorectal carcinoma.
We collected a total of 78 tissue specimens, including primary colorectal carcinoma, normal colonic mucosa, and liver metastases of colon carcinoma, to examine whether the extracts of these tissues inhibited the binding of a monoclonal antibody FH6, specific for sialyl-dimeric LeX antigen. The results of inhibition assays demonstrated that: (a) contents of FH6-reactive molecules were greater in carcinoma tissues than in normal colonic mucosa; (b) metastatic foci in livers contained more FH6-reactive molecules than primary tumors; (c) primary tumors from Dukes' stage B1 patients contained less FH6-reactive molecules than primary tumors from Dukes' stage D patients. The inhibitory activity of these tumor tissue extracts against the binding of a monoclonal antibody FH6 to cultured colon carcinoma cells was eliminated by prior treatment of the extracts with sialidase, confirming that the FH6-reactive materials were sialyl-dimeric LeX antigen. Electrophoretic separation of tumor tissue extracts on 3% polyacrylamide gels followed by direct staining with monoclonal antibody FH6 revealed that very high molecular weight glycoproteins, presumably mucins, contained sialyl-dimeric LeX antigen.
Hoff
S D
SD
Department of General Surgery, University of Texas M. D. Anderson Cancer Center, Houston 77030.
Matsushita
Y
Y
Ota
D M
DM
Cleary
K R
KR
Yamori
T
T
Hakomori
S
S
Irimura
T
T
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis*; D001667:Binding, Competitive; D015179:Colorectal Neoplasms / Q000276:immunology* / Q000473:pathology; D004591:Electrophoresis, Polyacrylamide Gel; D006017:Glycolipids / Q000032:analysis*; D006801:Humans; D007202:Indicators and Reagents; D007413:Intestinal Mucosa / Q000276:immunology; D016256:Lewis X Antigen; D008113:Liver Neoplasms / Q000276:immunology* / Q000556:secondary; D009439:Neuraminidase
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D006017:Glycolipids; D007202:Indicators and Reagents; D016256:Lewis X Antigen; D009439:Neuraminidase