title: Characterization of two possible forms of type IV collagen from human kidney cortex.
abstract: Type IV collagen was isolated from human kidney cortex according to the purification of mouse kidney type IV collagen (MKIVC) with minor modifications as described previously [Oikawa, T., et al. (1986) Chem. Pharm. Bull. 34, 789-797]. Chromatography of human kidney type IV collagen (HKIVC) on a column of DEAE-cellulose resulted in its separation into two fractions, i.e., one (HKIVC-1) passed through and the other (HKIVC-2) adsorbed to the column, similar to MKIVC. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Bio-Gel A-5m chromatography revealed that both HKIVC-1 and -2 differed from each other in the ratios of major and minor components. Amino acid analyses also demonstrated that there was a significant difference in the contents of several amino acid residues between both type IV collagens and their 80-kDa components, one of the major ones. These results indicate the possibility that there exist two forms of type IV collagen in human kidney.
authors.affiliation: Division of Cancer Therapeutics, Tokyo Metropolitan Institute of Medical Science, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Oikawa
authors.forename: K
authors.initials: K
authors.lastname: Sayama
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuda
authors.forename: Y
authors.initials: Y
authors.lastname: Fujimoto
authors.forename: T
authors.initials: T
authors.lastname: Iwaguchi
authors.forename: A
authors.initials: A
authors.lastname: Matsuzawa
mesh_terms: D000596:Amino Acids / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods; D003094:Collagen / Q000145:classification / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007672:Kidney Cortex / Q000032:analysis*; D003012:Microbial Collagenase
chemical_list: D000596:Amino Acids; D003094:Collagen; D003012:Microbial Collagenase