title: Modulation of adenylate cyclase activity in bovine lens epithelial cells.
abstract: Adenylate cyclase activity of bovine lens epithelial cells is activated by guanine nucleotides or fluoride. Additional activation to that of guanylylimidodiphosphate [Gpp(NH)p] was induced by isoproterenol. Insulin was found to inhibit adenylate cyclase activity. Adenosine at low concentrations (5-50 nM) increased adenylate cyclase activity, while at high concentrations it lowered the activity of the enzyme. The occurrence of R and P adenosine sites in lens epithelial cells is suggested. Like other tissues, lens epithelial cells possess a transduction system (adenylate cyclase-G protein complex) for external messages.
authors.affiliation: Centre de Neurochimie du CNRS, Strasbourg, France.
authors.forename: J C
authors.initials: JC
authors.lastname: Bizec
authors.forename: J
authors.initials: J
authors.lastname: Klethi
authors.forename: P
authors.initials: P
authors.lastname: Mandel
mesh_terms: D000241:Adenosine / Q000494:pharmacology; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D000242:Cyclic AMP / Q000032:analysis; D006152:Cyclic GMP / Q000032:analysis; D004837:Epinephrine / Q000494:pharmacology; D004847:Epithelial Cells; D005459:Fluorides / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D007545:Isoproterenol; D007908:Lens, Crystalline / Q000201:enzymology*; D009924:Organ Culture Techniques; D010727:Phosphoric Diester Hydrolases / Q000032:analysis
chemical_list: D007328:Insulin; D000242:Cyclic AMP; D010727:Phosphoric Diester Hydrolases; D000262:Adenylyl Cyclases; D006152:Cyclic GMP; D000241:Adenosine; D007545:Isoproterenol; D005459:Fluorides; D004837:Epinephrine