title: Stereological and functional investigations on isolated adrenocortical cells: zona fasciculata/reticularis cells of chronically ACTH-treated rats.
abstract: The morphology and function of isolated inner (zona fasciculata/reticularis) adrenocortical cells of rats pretreated with ACTH for 3, 6, 9 or 12 days were investigated. ACTH treatment induced a notable time-dependent enhancement in the steroidogenic capacity (corticosterone production) and growth of inner cells. The volumes of cells, mitochondrial compartment, membrane space [the cellular space occupied by smooth endoplasmic reticulum (SER) membranes] and lipid-droplet compartment, as well as the surface area of mitochondrial cristae and SER tubules, were increased in relation to the duration of ACTH pretreatment, and showed a highly significant positive linear correlation with both basal and stimulated corticosterone production. The acute exposure of isolated cells to ACTH provoked a striking lipid-droplet depletion, the extent of which was linearly and positively correlated with stimulated corticosterone secretion. The hypertrophy of the mitochondrial compartment and SER are interpreted as the morphological counterpart of the enhanced steroidogenic capacity of inner adrenocortical cells, inasmuch as the enzymes of steroid synthesis are located in these two organelles, and it is well known that chronic ACTH exposure stimulates the de novo synthesis of many of them in vivo. The rise in the number of lipid droplets, in which cholesterol is stored, is interpreted as being due to the fact that, under chronic ACTH treatment, the processes leading to cholesterol accumulation in adrenocortical cells (exogenous uptake and endogenous synthesis) exceed those of its utilization in basal steroid secretion. Cholesterol accumulated in lipid droplets as a reserve material may be rapidly utilized after acute ACTH exposure to meet the needs of the enhanced steroidogenic capacity of adrenocortical cells.
authors.affiliation: Department of Anatomy, University of Padua, Italy.
authors.forename: P G
authors.initials: PG
authors.lastname: Andreis
authors.forename: P
authors.initials: P
authors.lastname: Rebuffat
authors.forename: A S
authors.initials: AS
authors.lastname: Belloni
authors.forename: G
authors.initials: G
authors.lastname: Neri
authors.forename: L
authors.initials: L
authors.lastname: Cavallini
authors.forename: G
authors.initials: G
authors.lastname: Gottardo
authors.forename: G
authors.initials: G
authors.lastname: Mazzocchi
authors.forename: A
authors.initials: A
authors.lastname: Coi
authors.forename: L K
authors.initials: LK
authors.lastname: Malendowicz
authors.forename: G G
authors.initials: GG
authors.lastname: Nussdorfer
mesh_terms: D000302:Adrenal Cortex / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology*; D000818:Animals; D003345:Corticosterone / Q000096:biosynthesis; D003627:Data Interpretation, Statistical; D004721:Endoplasmic Reticulum / Q000187:drug effects; D050356:Lipid Metabolism; D008297:Male; D008854:Microscopy, Electron; D008928:Mitochondria / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D015383:Zona Fasciculata / Q000187:drug effects; D015385:Zona Reticularis / Q000187:drug effects
chemical_list: D000324:Adrenocorticotropic Hormone; D003345:Corticosterone