Hog cholera virus--characterization of specific antiserum and identification of cDNA clones.
A specific antiserum was raised against the pestivirus inducing hog cholera (hog cholera virus, HCV). Using immunoprecipitation and SDS-PAGE, this antiserum served for comparison of HCV-induced proteins with those from a related and better characterized pestivirus, bovine viral diarrhea virus (BVDV). In addition to immunological relationships, the apparent molecular weights of some proteins induced by both viruses were quite similar. HCV genomic RNA was found to be about 12 kb in length, comparable to BVDV RNA. cDNA was synthesized starting from RNA isolated from partially purified virions and cloned in lambda gt11. Screening with the antiserum resulted in identification of several positive clones. Partial sequencing of one HCV-derived cDNA clone revealed a high degree of homology to a portion of the BVDV sequence.
Rümenapf
T
T
Federal Research Centre for Virus Diseases of Animals, Tübingen, Federal Republic of Germany.
Meyers
G
G
Stark
R
R
Thiel
H J
HJ
D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000276:immunology; D001483:Base Sequence; D006692:Classical Swine Fever Virus / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D001908:Diarrhea Viruses, Bovine Viral / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011233:Precipitin Tests; D012367:RNA, Viral / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000276:immunology
D000914:Antibodies, Viral; D012367:RNA, Viral; D014764:Viral Proteins; D004247:DNA