Localization of c-ERB A proteins in rat liver using monoclonal antibodies.
Monoclonal antibodies were raised against the nuclear thyroid hormone receptors encoded by c-ERB A genes and against a purified nuclear receptor fraction. These antibodies recognize the c-ERB A protein in nuclear extracts from rat liver and are able to compete with thyroid hormone in Scatchard analyses. In sections of rat liver they react with all the hepatocyte nuclei as well as with the cells of the hepatic bile ducts. Comparison with another putative T3 receptor antibody, described previously, showed that distinct 57 kD proteins with a different cellular distribution were recognized.
Schmidt
E D
ED
Department of Anatomy and Embryology, Academic Medical Centre, Amsterdam, The Netherlands.
van Beeren
H C
HC
Korfage
H
H
Dussault
J H
JH
Wiersinga
W M
WM
Lamers
W H
WH
D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex / Q000032:analysis; D015153:Blotting, Western; D002467:Cell Nucleus / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D008099:Liver / Q000378:metabolism*; D010957:Plasmids; D011518:Proto-Oncogene Proteins / Q000032:analysis* / Q000235:genetics; D011519:Proto-Oncogenes; D051381:Rats; D011919:Rats, Inbred Strains; D011988:Receptors, Thyroid Hormone / Q000032:analysis*
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D011518:Proto-Oncogene Proteins; D011988:Receptors, Thyroid Hormone