Localization of the active type I DNA topoisomerase gene on human chromosome 20q11.2-13.1, and two pseudogenes on chromosomes 1q23-24 and 22q11.2-13.1.
Different subfragments of a cDNA coding for DNA topoisomerase I were used as probes to determine the chromosomal localization of topoisomerase I sequences in human cells. Southern blotting of restricted DNA from a panel of rodent-human somatic cell hybrids revealed the localization of the complete gene on chromosome 20 and the presence of two truncated topoisomerase I pseudogene sequences on chromosomes 1 and 22. In situ chromosome hybridization experiments confirmed these results showing the location of the complete gene on band q11.2-13.1 of chromosome 20, and the location of the pseudogene sequences on band q23-24 of chromosome 1 and q11.2-13.1 of chromosome 22.
Kunze
N
N
Fakultät für Biologie der Universität, Konstanz, Federal Republic of Germany.
Yang
G C
GC
Jiang
Z Y
ZY
Hameister
H
H
Adolph
S
S
Wiedorn
K H
KH
Richter
A
A
Knippers
R
R
D015139:Blotting, Southern; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D002890:Chromosomes, Human, Pair 20; D002892:Chromosomes, Human, Pair 22; D015342:DNA Probes; D004264:DNA Topoisomerases, Type I / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D005796:Genes; D006367:HeLa Cells / Q000201:enzymology; D006801:Humans; D009693:Nucleic Acid Hybridization; D011544:Pseudogenes
D015342:DNA Probes; D004273:DNA, Neoplasm; D004264:DNA Topoisomerases, Type I