Pancreas transplantation: an immunohistochemical analysis of pancreatic hormones and HLA-DR expression.
The immunohistochemical features of pancreatic grafts in eight patients with pancreatic transplants were analyzed and compared with pancreases from five patients with chronic pancreatitis and with three pancreatic tissues without histological abnormalities. There was a significant increase in glucagon producing cells in patients with transplanted pancreases compared with those with chronic pancreatitis (P less than 0.05). A significant decline in insulin-producing cells was seen in the transplanted pancreases with rejection in comparison with normal pancreatic tissue. Immunohistochemical staining for HLA-DR (Ia) antigens revealed expression of HLA-DR by endothelial cells, mononuclear cells, and by some ductal epithelial cells, but not by the endocrine islet cells. These results suggest that significant changes in insulin and glucagon production occur in the transplanted pancreas with rejection and that HLA-DR is not expressed by islet cells during graft rejection or with chronic inflammation.
Lloyd
R V
RV
Department of Pathology, University of Michigan Medical Center, Ann Arbor.
Dafoe
D C
DC
Campbell
D A
DA
Merion
R M
RM
Turcotte
J G
JG
Vinik
A I
AI
D000328:Adult; D002908:Chronic Disease; D003922:Diabetes Mellitus, Type 1 / Q000601:surgery; D005260:Female; D005934:Glucagon / Q000378:metabolism; D006084:Graft Rejection; D006684:HLA-DR Antigens / Q000032:analysis*; D006801:Humans; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D010179:Pancreas / Q000378:metabolism / Q000648:ultrastructure; D016035:Pancreas Transplantation; D010187:Pancreatic Hormones / Q000378:metabolism*; D010191:Pancreatic Polypeptide / Q000378:metabolism; D010195:Pancreatitis / Q000378:metabolism / Q000473:pathology; D013004:Somatostatin / Q000378:metabolism; D013601:T-Lymphocytes / Q000145:classification
D006684:HLA-DR Antigens; D007328:Insulin; D010187:Pancreatic Hormones; D013004:Somatostatin; D010191:Pancreatic Polypeptide; D005934:Glucagon