Microspectrofluorometric evaluation of single- and double-stranded DNA in short-term cultured human glioma cells.
Seven cerebral gliomas in short-term culture were studied by microspectrofluorometry and acridine orange staining to assess their nuclear content of single- and double-stranded DNA. Benign gliomas showed a diploid DNA pattern, whereas malignant gliomas revealed a higher frequency of DNA aneuploidy and hyperploidy. The content of single-stranded DNA remained relatively low in benign gliomas; however, that in malignant gliomas varied widely. After ACNU treatment, the double-stranded DNA histograms showed S-phase-specific accumulation in all cases but 1 with increasing concentrations of ACNU. The single-stranded DNA content decreased considerably in 2 cases, which responded well to chemotherapy and showed clinical amelioration.
Itoh
Y
Y
Department of Neurosurgery, Akita University School of Medicine, Japan.
Mineura
K
K
Fushimi
S
S
Ishino
Y
Y
Kowada
M
M
D000328:Adult; D001932:Brain Neoplasms / Q000032:analysis / Q000235:genetics*; D002648:Child; D004247:DNA / Q000032:analysis; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005910:Glioma / Q000032:analysis / Q000235:genetics*; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D015376:Nimustine / Q000494:pharmacology; D011123:Polyploidy; D013050:Spectrometry, Fluorescence; D014407:Tumor Cells, Cultured / Q000032:analysis* / Q000187:drug effects
D004273:DNA, Neoplasm; D015376:Nimustine; D004247:DNA