Transient forebrain ischemia produces multiple deficits in dopamine D1 transmission in the lateral neostriatum of the rat.
Striatal dopamine D1 transmission was studied in rats 7 days after transient (30 min) forebrain ischemia using the 4-vessel occlusion model. The striatal distribution of dopamine D1 ([3H]SCH 23390 binding sites) and D2 ([3H]sulpiride binding sites) receptors as well as the distribution of adenylate cyclase ( [3H]forskolin binding sites) and of the intracytoplasmic dopamine and cAMP-regulated phosphoprotein DARPP-32 related to D1 transmission were analyzed. While the distribution of D2 receptors was unaffected 7 days after the ischemic insult, all the other markers showed a patchy disappearance in the dorsolateral part of the neostriatum. These findings underline the existence of selective multiple deficits in D1 transmission after transient forebrain ischemia in rat striatum.
Benfenati
F
F
Institute of Human Physiology, University of Modena, Italy.
Pich
E M
EM
Grimaldi
R
R
Zoli
M
M
Fuxe
K
K
Toffano
G
G
Agnati
L F
LF
D000818:Animals; D001345:Autoradiography; D001552:Benzazepines / Q000378:metabolism; D001665:Binding Sites; D002545:Brain Ischemia / Q000378:metabolism*; D005576:Colforsin / Q000378:metabolism; D003342:Corpus Striatum / Q000378:metabolism*; D004027:Diencephalon / Q000098:blood supply; D018492:Dopamine Antagonists; D051937:Dopamine and cAMP-Regulated Phosphoprotein 32; D007150:Immunohistochemistry; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011954:Receptors, Dopamine / Q000502:physiology*; D017447:Receptors, Dopamine D1; D013469:Sulpiride / Q000378:metabolism; D009435:Synaptic Transmission; D013687:Telencephalon / Q000098:blood supply
D001552:Benzazepines; D018492:Dopamine Antagonists; D051937:Dopamine and cAMP-Regulated Phosphoprotein 32; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; D011954:Receptors, Dopamine; D017447:Receptors, Dopamine D1; D005576:Colforsin; D013469:Sulpiride