Comparison of human papillomavirus type 1 serotyping by monoclonal antibodies with genotyping by in situ hybridization of plantar warts.
Thirty plantar warts were analyzed for the presence of HPV-1 type-specific and PV genus-specific capsid antigens by immunofluorescence (IF) using monoclonal and polyclonal antibodies and type-specific HPV-1 DNA employing in situ hybridization methods. Fifteen of 30 plantar warts were positive by IF for PV genus-specific structural viral antigens. Thirteen of the 15 productively infected plantar warts expressed intranuclear HPV-1 type-specific capsid antigens and viral DNA, which were detected in the same distribution in each individual wart. The 2 productively infected plantar warts that did not react with HPV-1 type-specific MoAbs did not react with HPV-1 type-specific DNA by in situ hybridization. Thus, serotyping of HPV-1 capsid antigens by monoclonal antibodies is concordant with genotyping of HPV-1 viral DNA by in situ hybridization in productively infected plantar warts.
Jenson
A B
AB
Department of Pathology, Georgetown University School of Medicine, Washington DC 20007.
Lim
L Y
LY
Singer
E
E
D000911:Antibodies, Monoclonal; D004279:DNA, Viral / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D005534:Foot Diseases / Q000235:genetics*; D005838:Genotype; D006801:Humans; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000145:classification*; D012703:Serotyping / Q000379:methods; D014860:Warts / Q000235:genetics*
D000911:Antibodies, Monoclonal; D004279:DNA, Viral