title: Cyclic guanosine 3',5' monophosphate mediates the inhibitory effect of atrial natriuretic factor in adrenergic, neuronal pheochromocytoma cells.
abstract: This study tests the hypothesis that atrial natriuretic factor (ANF) inhibits catecholamine release from rat pheochromocytoma cells by increasing levels of intracellular cyclic GMP (cGMP). Rat differentiated pheochromocytoma cells are a model of adrenergic nerves and allow the exploration of the effects of various hormones, autacoids, drugs and neuromodulators on adrenergic neurotransmission in cell culture. Synthetic rat ANF (99-126) inhibited K+-induced norepinephrine and dopamine release, as measured by high-performance liquid chromatography, in a concentration-dependent manner over the concentration range of 10(-11) to 10(-8) M. ANF stimulated intracellular cGMP accumulation, as measured by specific radioimmunoassay, in a concentration-dependent manner over the same concentration range. The cGMP analog, N2-2'-O-dibutyryl cGMP also inhibited K+-induced norepinephrine and dopamine release in a concentration-dependent manner. The results of this study are consistent with the hypothesis that ANF acts as an inhibitory neuromodulator in adrenergic nerves via the second messenger, cGMP.
authors.affiliation: Department of Pharmacology, University of Minnesota-Duluth School of Medicine.
authors.forename: J G
authors.initials: JG
authors.lastname: Drewett
authors.forename: R J
authors.initials: RJ
authors.lastname: Ziegler
authors.forename: G R
authors.initials: GR
authors.lastname: Marchand
authors.forename: G J
authors.initials: GJ
authors.lastname: Trachte
mesh_terms: D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D002395:Catecholamines / Q000378:metabolism*; D006152:Cyclic GMP / Q000032:analysis / Q000502:physiology*; D003995:Dibutyryl Cyclic GMP / Q000494:pharmacology; D010673:Pheochromocytoma / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D014407:Tumor Cells, Cultured
chemical_list: D002395:Catecholamines; D003995:Dibutyryl Cyclic GMP; D009320:Atrial Natriuretic Factor; D006152:Cyclic GMP; D011188:Potassium