Two components of type III protein kinase C with different substrate specificities and a phospholipid-dependent but Ca2+-inhibited protein kinase in rat brain.
The activities of rat brain protein kinase C isoenzymic fractions separated by hydroxyapatite chromatography were measured with histone H1 or the oligopeptide Ala-Ala-Ala-Ser-Phe-Lys-Ala-Lys-Lys-amide as substrates. The oligopeptide was a better substrate than histone H1 for nearly all of the protein kinase C fractions. Two subfractions of type III isoenzyme were resolved (IIIa and IIIb); type IIIb was characterized by a very low histone kinase activity compared to its peptide kinase activity. In some brain extracts a phospholipid-dependent but Ca2+-inhibited protein kinase was also observed which was eluted from the hydroxyapatite column between type II and III isoenzymes of protein kinase C.
Buday
L
L
1st Institute of Biochemistry, Semmelweis University Medical School, Budapest, Hungary.
Mészáros
G
G
Farkas
G
G
Seprödi
J
J
Antoni
F
F
Faragó
A
A
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D006657:Histones / Q000378:metabolism; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009842:Oligopeptides / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D010770:Phosphotransferases / Q000378:metabolism; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D013379:Substrate Specificity
D006657:Histones; D007527:Isoenzymes; D009842:Oligopeptides; D010743:Phospholipids; D010770:Phosphotransferases; D011493:Protein Kinase C; D002118:Calcium