cDNA sequence for the alpha M subunit of the human neutrophil adherence receptor indicates homology to integrin alpha subunits.
The receptor on human neutrophils (polymorphonuclear leukocytes) that mediates cellular adherence consists of two noncovalently associated subunits, designated alpha M (Mac-1 alpha, Mol alpha, or CD11b; Mr, 170,000) and beta (Mac-1 beta, Mol beta, or CD18; Mr, 100,000). We isolated a cDNA clone for the human neutrophil alpha M subunit by screening a lambda gt 11 cDNA library made from chronic myelogenous leukemia neutrophils by using an affinity-purified rabbit polyclonal antibody directed against the alpha M subunit. We used this cDNA clone to obtain additional clones from cDNA libraries made from differentiated HL-60 promyelocytic leukemia cells. Together these cDNAs constitute the complete 1137-amino acid sequence for the mature human alpha M subunit protein. The deduced amino acid sequence indicates the presence of an extensive extracellular domain with three putative metal-binding regions, (i) an amino acid region that is homologous to the A domain of von Willebrand factor, (ii) a 26-amino acid hydrophobic sequence that is a potential transmembrane domain, and (iii) a 19-amino acid cytoplasmic region. The amino acid sequence for the human neutrophil alpha M subunit contains regions that are closely related to amino acid sequences of adhesion receptors belonging to the integrin family.
Hickstein
D D
DD
Medical Research Division, Seattle Veterans Administration Medical Center, WA 98108.
Hickey
M J
MJ
Ozols
J
J
Baker
D M
DM
Back
A L
AL
Roth
G J
GJ
D000595:Amino Acid Sequence; D000943:Antigens, Differentiation / Q000235:genetics*; D001483:Base Sequence; D001798:Blood Proteins / Q000235:genetics*; D015152:Blotting, Northern; D018821:CD18 Antigens; D003062:Codon / Q000235:genetics; D004247:DNA / Q000235:genetics*; D005796:Genes; D006801:Humans; D046911:Macromolecular Substances; D016177:Macrophage-1 Antigen; D008562:Membrane Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000276:immunology*; D011971:Receptors, Immunologic / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D000943:Antigens, Differentiation; D001798:Blood Proteins; D018821:CD18 Antigens; D003062:Codon; D046911:Macromolecular Substances; D016177:Macrophage-1 Antigen; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic; D004247:DNA