A monoclonal antibody which inhibits H+/K(+)-ATPase activity but not chloride conductance.
A mouse monoclonal antibody was raised against hog gastric membranes. This antibody (95-111 mAb) has a very high affinity for the 95 kDalton band of H+/K(+)-ATPase-enriched membranes, and does not react with Na+/K(+)-ATPase. The epitope is located on the tubulovesicles and canaliculi of the parietal cells. The 95-111 mAb also inhibits the ATP hydrolytic activity, decreases the steady-state phosphorylation level and inhibits the phosphatase activity of H+/K(+)-ATPase, strongly suggesting that the epitope is on the catalytic subunit of H+/K(+)-ATPase. The 95-111 mAb also recognizes rat, rabbit and human gastric H+/K(+)-ATPase. This mAb differs from the H+/K(+)-ATPase-inhibiting mAb previously described (Asano et al. (1987) J. Biol. Chem. 262, 13263-13268), in that it does not inhibit the chloride conductance opened by Cu-o-phenanthroline in gastric vesicles.
Benkouka
F
F
INSERM Unité 10, Ancien Hôpital Bichat, Paris, France.
Péranzi
G
G
Robert
J C
JC
Lewin
M J
MJ
Soumarmon
A
A
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors* / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000941:Antigens / Q000032:analysis; D001667:Binding, Competitive; D015153:Blotting, Western; D002712:Chlorides / Q000378:metabolism*; D004553:Electric Conductivity; D004591:Electrophoresis, Polyacrylamide Gel; D005753:Gastric Mucosa / Q000201:enzymology* / Q000276:immunology; D017506:H(+)-K(+)-Exchanging ATPase; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008861:Microsomes / Q000201:enzymology; D011817:Rabbits; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013552:Swine
D000911:Antibodies, Monoclonal; D000941:Antigens; D002712:Chlorides; D000251:Adenosine Triphosphatases; D017506:H(+)-K(+)-Exchanging ATPase