Human granulocytes and granulocytes from other species demonstrate differences in chemotactic responsiveness to oxidized N-formyl-methionyl-leucyl-phenylalanine.
1. Oxidation of the methionine of N-formyl-methionyl-leucyl-phenylalanine to the sulfoxide or sulfone derivative results in the loss of the peptide's chemotactic activity for human granulocytes. 2. The oxidized peptides are chemotactic for human monocytes; however, 10- to 100-fold higher concentrations are required for optimal monocyte chemotaxis. 3. Mouse, guinea pig and rabbit granulocytes, and the WBC264-9 human-mouse hybrid cell line migrated to the oxidized peptides and required 10- to 1000-fold higher concentrations of the oxidized peptides to elicit optimal chemotactic responses. 4. Human granulocytes appear to be unique in their lack of responsiveness to oxidized derivatives.
Harvath
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Division of Blood and Blood Products, Food and Drug Administration, Bethesda, MD 20892.
Aksamit
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D000818:Animals; D002460:Cell Line; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D006098:Granulocytes / Q000187:drug effects* / Q000502:physiology; D006168:Guinea Pigs; D006801:Humans; D006822:Hybrid Cells / Q000502:physiology; D051379:Mice; D008815:Mice, Inbred Strains; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D010084:Oxidation-Reduction; D011817:Rabbits; D013045:Species Specificity
D009240:N-Formylmethionine Leucyl-Phenylalanine