Release of 2-thiobarbituric acid reactive products from glutamate or deoxyribonucleic acid by 1,2,4-benzenetriol or hydroquinone in the presence of copper ions.
Cytotoxic effects of various quinone compounds are thought to be due to the formation of semiquinone free radicals. Hydroquinone and 1,2,4-benzenetriol in the presence of copper ions release from glutamate or DNA aldehydic products capable of reacting with 2-thiobarbituric acid (TBA). The formation of TBA reactive products (TBAR) was greater in the presence of 1,2,4-benzenetriol in comparison with hydroquinone. Complete inhibition of formation of TBAR from glutamate by 1,2,4-benzenetriol and copper was observed in the presence of catalase, thiourea and mannitol. Albumin and superoxide dismutase offered substantial protection. Complete protection of formation of TBAR from DNA was observed in the presence of catalase and thiourea. Presence of albumin, mannitol and superoxide dismutase caused only partial inhibition. The formation of TBAR from glutamate or DNA is dependent on copper ion concentration. The present data indicate that hydroquinone and 1,2,4-benzenetriol in the presence of copper ions can lead to the formation of reactive hydroxyl radicals which can release TBAR from glutamate or DNA.
Rao
G S
GS
Industrial Toxicology Research Centre, Mahatma Gandhi Marg, Lucknow India.
Pandya
K P
KP
D000418:Albumins / Q000494:pharmacology; D000818:Animals; D002374:Catalase / Q000494:pharmacology; D002417:Cattle; D003300:Copper / Q000494:pharmacology*; D004247:DNA / Q000187:drug effects / Q000378:metabolism*; D004249:DNA Damage; D004357:Drug Synergism; D005609:Free Radicals; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D006873:Hydroquinones / Q000633:toxicity*; D066298:In Vitro Techniques; D008353:Mannitol / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000494:pharmacology; D013858:Thiobarbiturates / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013890:Thiourea / Q000494:pharmacology
D000418:Albumins; D005609:Free Radicals; D005971:Glutamates; D006873:Hydroquinones; D013858:Thiobarbiturates; C013038:hydroxyhydroquinone; D018698:Glutamic Acid; D008353:Mannitol; D003300:Copper; D004247:DNA; D002374:Catalase; D013482:Superoxide Dismutase; D013890:Thiourea; C029684:thiobarbituric acid; C031927:hydroquinone