Immunoaffinity isolation of Na,K-ATPase alpha 3 isoform from pig kidney.
The Na,K-ATPase alpha 3 isoform of the catalytic subunit has been isolated from pig kidney microsomes. The procedure employs immunoaffinity chromatography on Sepharose 4B covalently coupled with monospecific antibodies a-II against the synthetic peptide including the putative alpha 3 N-terminus. The structural analysis provides unambiguous proof that the isolated protein corresponds to the third transcript for the alpha 3 isoform. The N-terminal amino acid sequence determined. Met-Gly-Asp-Lys-Lys-Asp-Asp, shows that unlike the alpha 1 and alpha 2 proteins, the mature Na,K-ATPase isoform lacks post-translational proteolytic processing.
Arystarkhova
E A
EA
Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, Moscow.
Lakhtina
O E
OE
Levina
N B
NB
Modyanov
N N
NN
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D007527:Isoenzymes / Q000302:isolation & purification*; D007668:Kidney / Q000201:enzymology*; D008861:Microsomes / Q000201:enzymology; D008969:Molecular Sequence Data; D000254:Sodium-Potassium-Exchanging ATPase / Q000302:isolation & purification*; D013552:Swine
D000906:Antibodies; D000936:Antigen-Antibody Complex; D007527:Isoenzymes; D000254:Sodium-Potassium-Exchanging ATPase