The murine retinoblastoma homolog maps to chromosome 14 near Es-10.
Restriction fragment length variants have been exploited to map genetically Rb-1, the murine homolog of the human retinoblastoma gene. Rb-1 localized to mouse chromosome 14 on the basis of results from analysis of somatic cell hybrids. In an interspecific backcross involving Mus spretus, Rb-1 and the murine homolog of the human esterase D gene (ESD), which we refer to here as Esd, were inseparable. Furthermore, the strain distribution patterns of Rb-1 and Es-10 are the same in 31 of 32 recombinant inbred strains. Close linkage of the chromosome 14 morphological marker hairless (hr) to Rb-1 is also implied. These results localize Rb-1 on the mouse linkage map and provide close genetic markers to follow Rb-1 in somatic as well as in germline genetic experiments. Additionally, the results suggest that Es-10 is the murine homolog of ESD and provide further evidence for linkage conservation during mammalian evolution.
Stone
J C
JC
Jackson Laboratory, Bar Harbor, Maine 04609.
Crosby
J L
JL
Kozak
C A
CA
Schievella
A R
AR
Bernards
R
R
Nadeau
J H
JH
D000818:Animals; D005075:Biological Evolution; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000235:genetics; D002874:Chromosome Mapping; D006224:Cricetinae; D003433:Crosses, Genetic; D015342:DNA Probes; D004195:Disease Models, Animal; D005260:Female; D008040:Genetic Linkage; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009115:Muridae / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D012175:Retinoblastoma / Q000235:genetics*
D015342:DNA Probes; D005819:Genetic Markers; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; C020118:ESD protein, human