1H NMR study of the solution properties of the polypeptide neurotoxin I from the sea anemone Stichodactyla helianthus.
The solution properties of the polypeptide neurotoxin I from the sea anemone Stichodactyla helianthus (Sh I) have been investigated by high-resolution 1H nuclear magnetic resonance (NMR) spectroscopy at 300 MHz. The pH dependence of the spectra has been examined over the range 1.1-12.2 at 27 degrees C. Individual pKa values have been obtained for the alpha-ammonium group of Ala-1 (8.6) and the side chains of Glu-8 (3.7), Tyr-36 (10.9), and Tyr-37 (10.8). For the remaining seven carboxyl groups in the molecule (from five Asp, Glu-31, and the C-terminus), four pKa values, viz., 2.8, 3.5, 4.1 and 6.4, can be clearly identified. The five Lys residues titrate in the range 10.5-11, but individual pKa values could not be obtained because of peak overlap. Conformational changes associated with the protonation of carboxylates occur below pH 4, while in the alkaline pH range major unfolding occurs above pH 10. The molecule also unfolds at elevated temperatures, having a transition temperature of ca. 55 degrees C at pH 5.25. Exchange of the backbone amide protons has been monitored at various values of pH and temperature in the ranges pH 4-5 and 12-27 degrees C. Up to 18 slowly exchanging amides are observed, consistent with the existence of a core of hydrogen-bonded secondary structure, most probably beta-sheet.(ABSTRACT TRUNCATED AT 250 WORDS)
Norton
R S
RS
School of Biochemistry, University of New South Wales, Kensington, Australia.
Cossins
A I
AI
Kem
W R
WR
D000595:Amino Acid Sequence; D000818:Animals; D003064:Cnidarian Venoms / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000302:isolation & purification; D011487:Protein Conformation; D012615:Sea Anemones; D012996:Solutions; D013816:Thermodynamics
D003064:Cnidarian Venoms; D009498:Neurotoxins; D012996:Solutions; C059713:neurotoxin I, Stichodactyla helianthus