title: [Constitutive expression of human fibroblast interferon gene controlled by SV40 early promoter in CHO cells].
abstract: HindII fragment encoding human fibroblast interferon (IFN beta) gene coding sequence was fused at 60 bp downstream from the RNA start site of SV40 early gene to be a constitutive expression plasmid pSVE beta. This recombinant plasmid was transfected into the dihydrofolate reductase (dhfr)-deficient Chinese hamster ovary (CHO) cells together with a selectable dhfr gene. About half of transformants continuously secreted IFN beta into the supernatant without inducement. One of the subclone transformants constitutively produced up to 852U IFN beta/2 X 10(6) cells/ml. 48hr in common medium.
authors.affiliation: Institute of Microbiology and Epidemiology, Academy of Military Medical Science, Beijing.
authors.forename: X M
authors.initials: XM
authors.lastname: Wang
authors.forename: M
authors.initials: M
authors.lastname: Yu
authors.forename: J X
authors.initials: JX
authors.lastname: Wang
authors.forename: S H
authors.initials: SH
authors.lastname: Wu
authors.forename: Y W
authors.initials: YW
authors.lastname: Hu
authors.forename: Y D
authors.initials: YD
authors.lastname: Hou
mesh_terms: D000818:Animals; D006224:Cricetinae; D003412:Cricetulus; D005786:Gene Expression Regulation; D006801:Humans; D007370:Interferon Type I / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D013539:Simian virus 40 / Q000235:genetics*
chemical_list: D007370:Interferon Type I