title: Nucleotide sequence of gene segment 1 of a porcine rotavirus strain.
abstract: The nucleotide sequence of gene segment 1, which encodes VP1 of porcine rotavirus strain Gottfried, was determined. VP1 is associated with single-shelled rotavirus particles and has been linked to virus transcriptase and replicase enzymatic activities. Gene segment 1 is 3302 nucleotides long with a single open reading frame capable of coding for a protein of 1088 amino acids (calculated mol wt 125 kDa). The predicted amino acid sequence revealed that VP1 is basic, with a net positive charge of 18 at pH 7.0. It shares five consensus sequences with several well-characterized RNA-dependent RNA polymerases. Gottfried VP1 also shares consensus sequences with certain GTP-binding proteins; however, we could not detect any GTP-binding activity in VP1. Our preliminary experiments suggest that VP3, another polypeptide located in single-shelled rotavirus particles, possesses GTP-binding activity. These results suggest that mRNA synthesis and capping enzyme activities are related to VP1 and VP3, respectively.
authors.affiliation: Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
authors.forename: N
authors.initials: N
authors.lastname: Fukuhara
authors.forename: K
authors.initials: K
authors.lastname: Nishikawa
authors.forename: M
authors.initials: M
authors.lastname: Gorziglia
authors.forename: A Z
authors.initials: AZ
authors.lastname: Kapikian
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics*; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics*; D012401:Rotavirus / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral; D014764:Viral Proteins