title: Induction of CAD gene amplification by restriction endonucleases in V79,B7 Chinese hamster cells.
abstract: The restriction endonucleases PvuII, BamHI and EcoRI were tested for their ability to induce gene amplification in V79,B7 Chinese hamster cells. The results indicate that treatment with these enzymes efficiently increases the frequency of clones resistant to N-phosphonacetyl-L-aspartate, indicating induction of CAD gene amplification.
authors.affiliation: Department of Cell and Developmental Biology A. Monroy, Palermo, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Cavolina
authors.forename: C
authors.initials: C
authors.lastname: Agnese
authors.forename: A
authors.initials: A
authors.lastname: Maddalena
authors.forename: G
authors.initials: G
authors.lastname: Sciandrello
authors.forename: A
authors.initials: A
authors.lastname: Di Leonardo
mesh_terms: D000581:Amidohydrolases / Q000235:genetics*; D000818:Animals; D001221:Aspartate Carbamoyltransferase / Q000235:genetics*; D001224:Aspartic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D002223:Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) / Q000235:genetics*; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004080:Dihydroorotase / Q000235:genetics*; D005784:Gene Amplification; D005796:Genes; D009097:Multienzyme Complexes / Q000235:genetics*; D009152:Mutagenicity Tests; D009363:Neoplasm Proteins / Q000235:genetics; D009943:Organophosphorus Compounds / Q000494:pharmacology*; D010746:Phosphonoacetic Acid / Q000031:analogs & derivatives / Q000494:pharmacology*
chemical_list: C033626:CAD trifunctional enzyme; D009097:Multienzyme Complexes; D009363:Neoplasm Proteins; D009943:Organophosphorus Compounds; D001224:Aspartic Acid; C013195:sparfosic acid; D001221:Aspartate Carbamoyltransferase; D004262:DNA Restriction Enzymes; D000581:Amidohydrolases; D004080:Dihydroorotase; D002223:Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing); D010746:Phosphonoacetic Acid