Metal-specific posttranscriptional control of human metallothionein genes.
During the initial 4 h of treatment, copper and zinc similarly activated the rates of transcription and mRNA accumulation from the two human metallothionein (MT) genes, viz., MTI-G and MTII-A, in the hepatoblastoma cell line HepG2. The levels of copper-induced MT mRNAs remained at a plateau for up to 15 h. In contrast, the levels of zinc-induced MT mRNAs gradually declined after about 4 h, despite substantial transcription. The decrease in the zinc-induced MT mRNA half-life is probably due to a posttranscriptional event(s).
Sadhu
C
C
Department of Biological Sciences, University of Calgary, Alberta, Canada.
Gedamu
L
L
D000199:Actins / Q000235:genetics; D006528:Carcinoma, Hepatocellular; D002460:Cell Line; D003300:Copper / Q000494:pharmacology*; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects*; D005796:Genes; D006801:Humans; D007700:Kinetics; D008113:Liver Neoplasms; D008668:Metallothionein / Q000096:biosynthesis / Q000235:genetics*; D012323:RNA Processing, Post-Transcriptional / Q000187:drug effects*; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects; D015032:Zinc / Q000494:pharmacology*
D000199:Actins; D012333:RNA, Messenger; D003609:Dactinomycin; D003300:Copper; D008668:Metallothionein; D003513:Cycloheximide; D015032:Zinc