Spectrophotometric, electron paramagnetic resonance and oxygen binding studies on the hemoglobin from the marine polychaete Perinereis aibuhitensis (Grübe): comparative physiology of hemoglobin.
The physicochemical properties of giant hemoglobin (Hb) of the marine polychaete Perinereis aibuhitensis were extensively studied and the following results were obtained. (1) Light absorption spectra of the oxy, deoxy, CO, met, and cyanomet derivatives were similar to those for human Hb, except for a somewhat peculiar shape and pH-dependence of the met derivative, and high absorbance values around 277 nm for all these derivatives of Perinereis Hb. Abnormal pH dependence for the met derivative was confirmed by powder electron parmagnetic resonance (EPR) spectroscopy, which revealed that a water molecule does not coordinate to the heme iron as a sixth ligand. The high absorption around 277 nm is indicative of the existence of some non-heme polypeptide chains and/or a high content of aromatic residues in the molecule. (2) UV difference and derivative spectra revealed oxygenation-induced conformational changes in the protein moiety that are related to the degree of cooperativity. (3) The EPR spectrum for the nitrosyl derivative showed well-resolved triplet-triplet splittings due to 14N, indicating that the proximal residue is probably a histidine. (4) The oxygen affinity and cooperativity of this Hb were pH-dependent. Mg2+ markedly increased the oxygen affinity, the Bohr effect, and the cooperativity, which was maximal at physiological pH. CO2 and anions such as 2,3-diphosphoglycerate and inositol hexaphosphate had no effect on the oxygenation properties. Thus, different from vertebrate Hb, the oxygen-binding properties of this Hb are regulated by divalent cations which bind preferentially to the oxy form. The low temperature-dependence of oxygen affinity observed for this Hb is a sign of adaptation to the environment by this poikilothermic organism. (5) By using a graphic method, the minimal functional unit that preserves the full cooperativity (allosteric unit) was inferred to be the one containing 6 heme groups and its significance is discussed in connection with the structural hierarchy of the molecule.
Tsuneshige
A
A
Department of Physicochemical Physiology, Medical School, Osaka University.
Imai
K
K
Hori
H
H
Tyuma
I
I
Gotoh
T
T
D019794:2,3-Diphosphoglycerate; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002245:Carbon Dioxide / Q000494:pharmacology; D004163:Diphosphoglyceric Acids / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy; D006454:Hemoglobins / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000494:pharmacology; D010100:Oxygen / Q000502:physiology*; D010833:Phytic Acid / Q000494:pharmacology; D011077:Polychaeta / Q000378:metabolism*; D011485:Protein Binding; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013696:Temperature
D004163:Diphosphoglyceric Acids; D006454:Hemoglobins; D019794:2,3-Diphosphoglycerate; D002245:Carbon Dioxide; D010833:Phytic Acid; D008274:Magnesium; D010100:Oxygen; D002118:Calcium