title: A single point mutation confers tetrodotoxin and saxitoxin insensitivity on the sodium channel II.
abstract: A single point mutation of the rat sodium channel II reduces its sensitivity to tetrodotoxin and saxitoxin by more than three orders of magnitude. The mutation replaces glutamic acid 387 with a glutamine and has only slight effects on the macroscopic current properties, as measured under voltage-clamp in Xenopus oocytes injected with the corresponding cDNA-derived mRNA.
authors.affiliation: Department of Medical Chemistry, Kyoto University Faculty of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Noda
authors.forename: H
authors.initials: H
authors.lastname: Suzuki
authors.forename: S
authors.initials: S
authors.lastname: Numa
authors.forename: W
authors.initials: W
authors.lastname: Stühmer
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004553:Electric Conductivity; D008564:Membrane Potentials; D009154:Mutation; D051381:Rats; D012530:Saxitoxin / Q000494:pharmacology*; D015222:Sodium Channels / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D013329:Structure-Activity Relationship; D013779:Tetrodotoxin / Q000494:pharmacology*; D014982:Xenopus laevis
chemical_list: D015222:Sodium Channels; D012530:Saxitoxin; D013779:Tetrodotoxin