title: Cytochrome oxidase subunit II sequences in Petunia mitochondria: two intron-containing genes and an intron-less pseudogene associated with cytoplasmic male sterility.
abstract: The mitochondrial genome of Petunia hybrida contains two transcribed cytochrome oxidase subunit II (coxII) genes. The coding region of both genes is split by a 1.3 kb group II intron. Unlike coxII-1, which is similar to other sequenced plant coxII genes, the coxII-2 coding region is extended by 48 codons. The cytoplasmic male sterile (CMS) Petunia contains one coxII gene similar in structure and transcript pattern to the coxII-1 gene found in the fertile genome. Comparison of the sequenced coxII genes from the fertile mitochondrial genome with the coxII sequences present in the CMS-associated pcf gene from the CMS genome (Young and Hanson 1987) suggests that pcf is a processed pseudogene. A model for the generation of pcf is presented.
authors.affiliation: Section of Genetics and Development, Cornell University, Ithaca, NY 14853.
authors.forename: K D
authors.initials: KD
authors.lastname: Pruitt
authors.forename: M R
authors.initials: MR
authors.lastname: Hanson
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D017343:Genes, Plant; D007438:Introns; D008928:Mitochondria; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010944:Plants / Q000201:enzymology / Q000235:genetics*; D011544:Pseudogenes; D015183:Restriction Mapping
chemical_list: D004247:DNA; D003576:Electron Transport Complex IV