Glycogen-enzymes containing bodies in type 2 fibres of tenotomized muscles in the rat.
Inclusion bodies containing glycogen-enzymes were found in 30 to 60% of type 2 fibres of tenotomized calf muscles (m. gastrocnemius, m. soleus, m. plantaris) in rats, using histochemical reactions. The bodies appeared within 1 week after the tenotomy and were localized both in the central and the subsarcolemmal regions and rarely extruded into the extracellular space. These aggregates are 3 to 15 microns in length and 2 to 11 microns in diameter. In addition to glycogen, these bodies also contained various enzymes of the glycogen metabolism such as phosphorylase, a branching enzyme, and glucose-6-phosphatase, but showed no NADH-reductase, lactate dehydrogenase, or myofibrillar ATP-ase activity. The results indicate that glycogen-enzymes containing bodies are a degenerative phenomenon, which occurs only in type 2 fibres of the tenotomized muscles.
Józsa
L
L
Department of Morphology, National Institute of Traumatology, Budapest, Hungary.
Kvist
M
M
Lehto
M
M
Réffy
A
A
Vieno
T
T
D015061:1,4-alpha-Glucan Branching Enzyme / Q000032:analysis; D000818:Animals; D005260:Female; D005952:Glucose-6-Phosphatase / Q000032:analysis; D006003:Glycogen / Q000032:analysis*; D006651:Histocytochemistry; D002479:Inclusion Bodies / Q000201:enzymology*; D009132:Muscles / Q000201:enzymology*; D006005:Phosphorylases / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains / Q000033:anatomy & histology*
D006003:Glycogen; D006005:Phosphorylases; D015061:1,4-alpha-Glucan Branching Enzyme; D005952:Glucose-6-Phosphatase