Effects of active compounds isolated from Angelica shikokiana on lipid metabolism in fat cells.
It was found that the EtO Ac fraction of the roots of Angelica shikokiana inhibited adrenaline-induced lipolysis in rat fat cells, while having no effect on ACTH-induced lipolysis. On the other hand, the MeOH fraction was found to inhibit ACTH-induced lipolysis but not to inhibit adrenaline-induced lipolysis. The active substances isolated from this root were elucidated to be psoralen (I), bergapten (II), isopteryxin(3'-angeloyloxy-4'-acetoxy-3',4'-dihydroseseli n) (III) and 3'-epoxyangeloyloxy-4'-acetoxy-3',4'-dihydroseselin (IV), respectively. The two furocoumarins, psoralen and bergapten, were found to enhance ACTH- and adrenaline-induced lipolysis in fat cells respectively. In contrast, a coumarin derivative of khellactone type, isopteryxin (III), was found to inhibit the adrenaline-induced lipolysis but with no effect on ACTH-induced lipolysis and insulin-induced lipogenesis from glucose. The other coumarin of khellactone type, 3'(R), 4'(R)-3'-epoxyangeloyloxy-4'-acetoxy-3',4'-dihydroseseline (IV), was found to inhibit the insulin-induced lipogenesis from glucose but it had no effect on adrenaline- and ACTH-induced lipolysis.
Kimura
Y
Y
Second Department of Medical Biochemistry, School of Medicine, Ehime University, Japan.
Okuda
H
H
D000078223:5-Methoxypsoralen; D000273:Adipose Tissue / Q000187:drug effects* / Q000378:metabolism; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000818:Animals; D003374:Coumarins / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology; D005363:Ficusin / Q000494:pharmacology; D066298:In Vitro Techniques; D050356:Lipid Metabolism; D008066:Lipolysis / Q000187:drug effects; D008730:Methoxsalen / Q000494:pharmacology; D015394:Molecular Structure; D010936:Plant Extracts / Q000494:pharmacology; D010946:Plants, Medicinal; D051381:Rats
D003374:Coumarins; D010936:Plant Extracts; D000078223:5-Methoxypsoralen; D000324:Adrenocorticotropic Hormone; D005363:Ficusin; D008730:Methoxsalen; D004837:Epinephrine