A kinetic analysis of lymphocyte 5'-nucleotidase in B-cell clones from control subjects and patients with primary hypogammaglobulinaemia.
The affinity of lymphocyte 5'-nucleotidase (5NT) for its substrate adenosine monophosphate (AMP) was studied in Epstein-Barr virus immortalized B-lymphocyte clones from healthy controls and patients with primary hypogammaglobulinaemia. Km values for AMP for 5NT in most of the patient clones were found to be significantly increased compared to B-cell clones from normal subjects, whereas Vmax values were within the normal range.
Barwick
S E
SE
Division of Clinical Cell Biology, MRC Clinical Research Centre, Harrow, Middlesex, UK.
Malkovsky
M
M
Webster
A D
AD
Peters
T J
TJ
D015720:5'-Nucleotidase / Q000032:analysis*; D000249:Adenosine Monophosphate / Q000378:metabolism; D000361:Agammaglobulinemia / Q000201:enzymology* / Q000473:pathology; D001402:B-Lymphocytes / Q000201:enzymology* / Q000473:pathology; D002461:Cell Line, Transformed; D004854:Herpesvirus 4, Human; D006801:Humans; D007700:Kinetics
D000249:Adenosine Monophosphate; D015720:5'-Nucleotidase