title: Long-term culture of peripheral blood stem cells: the effect of the addition of an irradiated stromal layer.
abstract: We have studied peripheral blood stem cells (PBSC) collected by cytapheresis following intensive chemotherapy, from 13 patients with acute leukemia, in long term culture (LTC). Peripheral blood was cultured with (n = 10) and without (n = 21) the addition of a preformed, irradiated stromal layer. In this latter LTC our results confirm that peripheral blood is capable of producing CFU-GM and nucleated cells in the absence of the formation of an adherent stromal layer. However, peripheral blood cultured in the presence of an irradiated stromal layer is capable of a significantly higher proliferative response (total production of CFU GM per flask - mean = 57529) than in the absence of an irradiated stromal layer (total production of CFU GM per flask - mean = 26739, p less than 0.03). Our results suggest that PBSC contain a primitive nonplastic adherent cell that requires the presence of a stromal layer for its expression. These findings provide further support for the use of peripheral blood stem cells for autologous transplantation.
authors.affiliation: Laboratory of Hematology, Hopital Haut Lévêque, CHR Bordeaux, France.
authors.forename: A
authors.initials: A
authors.lastname: Rice
authors.forename: P
authors.initials: P
authors.lastname: Bernard
authors.forename: C
authors.initials: C
authors.lastname: Foures
authors.forename: E
authors.initials: E
authors.lastname: Bascans
authors.forename: F
authors.initials: F
authors.lastname: Lacombe
authors.forename: G
authors.initials: G
authors.lastname: Marit
authors.forename: J
authors.initials: J
authors.lastname: Reiffers
mesh_terms: D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003470:Culture Media; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007937:Leukapheresis; D013997:Time Factors; D014182:Transplantation, Autologous
chemical_list: D003470:Culture Media