Binding of aromatic donor molecules to lactoperoxidase: proton NMR and optical difference spectroscopic studies.
The interaction of aromatic donor molecules with lactoperoxidase (LPO) was studied using 1H-NMR and optical difference spectroscopy techniques. pH dependence of substrate proton resonance line-widths indicated that the binding was facilitated by protonation of an amino acid residue (with pKa of 6.1) which is presumably a distal histidine. Dissociation constants evaluated from both optical difference spectroscopy and 1H-NMR relaxation measurements were found to be an order of magnitude larger than those for binding to horse radish peroxidase (HRP), indicating relatively weak binding of the donors to LPO. The dissociation constants evaluated in presence of excess of I- and SCN- showed a considerable increase in their values, indicating that the iodide and thiocyanate ions compete for binding at the same site. The dissociation constant of the substrate binding was, however, not affected by cyanide binding to the ferric centre of LPO. All these results indicate that the organic substrates bind to LPO away from the ferric center. Comparison of the dissociation constants between the different substrates suggested that hydrogen bonding of the donors with the distal histidine amino acid, and hydrophobic interaction between the donors and the active site contribute significantly towards the associating forces. Free energy, entropy and enthalpy changes associated with the LPO-substrate equilibrium have been evaluated. These thermodynamic parameters were found to be all negative and relatively low compared to those for binding to HRP. The distances of the substrate protons from the ferric center were found to be in the range 9.4-11.1 A which are 2-3 A larger than those reported for the HRP-substrate complexes. These structural informations suggest that the heme in LPO may be more deeply buried in the heme crevice than that in the HRP.
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Chemical Physics Group, Tata Institute of Fundamental Research, Colaba, Bombay, India.
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D000814:Aniline Compounds / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D005260:Female; D006735:Horseradish Peroxidase / Q000378:metabolism; D006358:Hot Temperature; D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D007784:Lactoperoxidase / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008892:Milk / Q000201:enzymology; D010544:Peroxidases / Q000378:metabolism*; D010636:Phenols / Q000378:metabolism*; D011522:Protons; D013057:Spectrum Analysis
D000814:Aniline Compounds; D010636:Phenols; D011522:Protons; D006735:Horseradish Peroxidase; D007784:Lactoperoxidase; D010544:Peroxidases