title: Failure to induce a DNA repair gene, RAD54, in Saccharomyces cerevisiae does not affect DNA repair or recombination phenotypes.
abstract: The Saccharomyces cerevisiae RAD54 gene is transcriptionally regulated by a broad spectrum of DNA-damaging agents. Induction of RAD54 by DNA-damaging agents is under positive control. Sequences responsible for DNA damage induction (the DRS element) lie within a 29-base-pair region from -99 to -70 from the most proximal transcription start site. This inducible promoter element is functionally separable from a poly(dA-dT) region immediately downstream which is required for constitutive expression. Deletions which eliminate induction of RAD54 transcription by DNA damage but do not affect constitutive expression have no effect on growth or survival of noninducible strains relative to wild-type strains in the presence of DNA-damaging agents. The DRS element is also not required for homothallic mating type switching, transcriptional induction of RAD54 during meiosis, meiotic recombination, or spontaneous or X-ray-induced mitotic recombination. We find no phenotype for a lack of induction of RAD54 message via the damage-inducible DRS, which raises significant questions about the physiology of DNA damage induction in S. cerevisiae.
authors.affiliation: Genetics Department, University of California, Berkeley 94720.
authors.forename: G M
authors.initials: GM
authors.lastname: Cole
authors.forename: R K
authors.initials: RK
authors.lastname: Mortimer
mesh_terms: D001483:Base Sequence; D004252:DNA Mutational Analysis; D004260:DNA Repair; D004262:DNA Restriction Enzymes; D005786:Gene Expression Regulation / Q000187:drug effects / Q000528:radiation effects; D005800:Genes, Fungal; D049770:Genes, Mating Type, Fungal; D005812:Genes, Switch; D008741:Methyl Methanesulfonate; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis; D011831:Radiation Genetics; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D014466:Ultraviolet Rays; D014965:X-Rays
chemical_list: D012333:RNA, Messenger; D008741:Methyl Methanesulfonate; D004262:DNA Restriction Enzymes