Direct visualisation of the soluble pool of tubulin in the neuronal growth cone: immunofluorescence studies following taxol polymerisation.
Previously we have shown that the predominant form of soluble alpha-tubulin in the growth cone is C-terminally tyrosinated (Gordon-Weeks and Lang. Dev. Brain Res., 42 (1988) 156-160). Here we show that when growth cones are incubated in taxol, this soluble pool of alpha-tubulin is polymerised onto the ends of the neurite microtubules that enter the proximal part of the growth cone indicating that it is assembly-competent.
Gordon-Weeks
P R
PR
Department of Anatomy and Human Biology, King's College, London, U.K.
Mansfield
S G
SG
Curran
I
I
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D003712:Dendrites / Q000378:metabolism*; D004622:Embryo, Mammalian; D007150:Immunohistochemistry; D008870:Microtubules / Q000378:metabolism*; D017239:Paclitaxel; D051381:Rats; D014404:Tubulin / Q000378:metabolism*
D000470:Alkaloids; D014404:Tubulin; D017239:Paclitaxel