title: ABH blood group antigen expression, synthesis, and degradation in human colonic adenocarcinoma cell lines.
abstract: The synthesis of blood group ABH antigens is under genetic control, where the primary gene products are glycosyltransferases. Several studies have demonstrated cancer-associated alterations in ABH antigen expression in human colon cancer tissues. However, the mechanism(s) responsible for these alterations has not been elucidated. Therefore, experiments were conducted using nine established colon cancer cell lines (four type O, three type A, and two type B) to examine ABH antigen expression by immunocytochemistry and correlate this with activities of ABH biosynthetic (glycosyltransferase) and degradative (glycosidase) enzymes. The products of the glycosyltransferase enzymes were characterized by high performance liquid chromatography and paper chromatography, and substrate affinities (apparent Km values) of the cancer cell-derived glycosyltransferases were analyzed. The present data demonstrate: (a) all cell lines except H-498 (blood type A) expressed the appropriate ABH glycosyltransferase as well as all three glycosidases; (b) product characterization and substrate dependence experiments suggested that the cancer cell-derived ABH glycosyltransferase enzymes had properties that were similar to those of the ABH enzymes in human serum; (c) H-498 cells exhibited A antigen deletion with accumulation of H precursor substance, most likely due to insufficient A transferase activity; (d) SW1417 cells (blood type B) demonstrated B antigen deletion without precursor accumulation, despite adequate levels of B transferase and low alpha-galactosidase activity; and (e) weak incompatible A antigen expression occurred in LoVo (type B) and SW1116 (type O) cells, and weak incompatible B antigen expression occurred in H-498 (type A) and SW1116 cells. However, since these cells lacked incompatible A or B transferase activity, these incompatible antigens are probably not the true A or B antigens. Thus, the colon cancer cell lines used in this study exhibit all of the ABH alterations previously described in colon cancer tissues and appear to be useful experimental models for studying the molecular events involved in cancer-associated ABH expression.
authors.affiliation: Gastrointestinal Research Laboratory, Veterans Administration Medical Center, San Francisco, California.
authors.forename: R
authors.initials: R
authors.lastname: Dahiya
authors.forename: S H
authors.initials: SH
authors.lastname: Itzkowitz
authors.forename: J C
authors.initials: JC
authors.lastname: Byrd
authors.forename: Y S
authors.initials: YS
authors.lastname: Kim
mesh_terms: D000017:ABO Blood-Group System / Q000276:immunology*; D000230:Adenocarcinoma / Q000201:enzymology / Q000276:immunology* / Q000378:metabolism; D003110:Colonic Neoplasms / Q000201:enzymology / Q000276:immunology* / Q000378:metabolism; D005700:Galactosyltransferases / Q000378:metabolism*; D006801:Humans; D017350:N-Acetylgalactosaminyltransferases; D014407:Tumor Cells, Cultured / Q000201:enzymology; D000519:alpha-Galactosidase / Q000378:metabolism; D000097763:Polypeptide N-acetylgalactosaminyltransferase
chemical_list: D000017:ABO Blood-Group System; D005700:Galactosyltransferases; D017350:N-Acetylgalactosaminyltransferases; D000519:alpha-Galactosidase; D000097763:Polypeptide N-acetylgalactosaminyltransferase