Comparison of monoclonal antibodies for rapid detection of cytomegalovirus in spin-amplified plate cultures.
Cytomegalovirus (CMV) was detected in 56 of 275 specimens (20%); 50 of 56 (89%) were detected by conventional culture, and 37 (66%) were detected by rapid assay at 72 h with a commercial monoclonal antibody and a pooled monoclonal antibody. Although the two antibodies were equally sensitive at 72 h, the pooled antibody gave a brighter, more easily detected signal. Other viruses were isolated from 9 specimens (3.3%) by conventional culture. Use of rapid assays alone fails to detect slow-growing CMV and non-CMV viral pathogens.
Ashley
R
R
Department of Laboratory Medicine, University of Washington, Seattle 98195.
Peterson
E
E
Abbo
H
H
Gold
D
D
Corey
L
L
D000911:Antibodies, Monoclonal; D003587:Cytomegalovirus / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D003588:Cytopathogenic Effect, Viral; D006801:Humans
D000911:Antibodies, Monoclonal