Evidence that the production of acetate in rat hepatocytes is a predominantly cytoplasmic process.
By using [1-14C]butyrate, the fluxes of butyrate to acetate and fatty acids were measured in rat hepatocytes. Both fluxes were inhibited to a similar extent by (-)-hydroxycitrate, with no significant effect on butyrate uptake. These results indicate that acetate formation takes place in the cytoplasm, presumably via ATP-stimulated acetyl-CoA hydrolase. Since acetate formation occurred despite a net uptake of acetate, the results are also consistent with the operation of a substrate cycle between acetate and acetyl-CoA, recently proposed by other workers, and suggest that this cycle is cytoplasmic.
Crabtree
B
B
Rowett Research Institute, Bucksburn, Aberdeen, U.K.
Souter
M J
MJ
Anderson
S E
SE
D000085:Acetates / Q000378:metabolism*; D000105:Acetyl Coenzyme A / Q000378:metabolism; D000104:Acetyl-CoA Hydrolase / Q000378:metabolism; D000818:Animals; D002087:Butyrates / Q000378:metabolism; D020148:Butyric Acid; D002951:Citrates / Q000494:pharmacology; D003593:Cytoplasm / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D006868:Hydrolysis; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D051381:Rats
D000085:Acetates; D002087:Butyrates; D002951:Citrates; D005227:Fatty Acids; D020148:Butyric Acid; D000105:Acetyl Coenzyme A; C007999:hydroxycitric acid; D000104:Acetyl-CoA Hydrolase