Construction of cloning/sequencing vectors with an alternative polylinker.
This paper describes the construction of a new plasmid and M13 phage cloning vector in which the 54-bp polylinkers of pUC19 and of M13mp8 are replaced with a 45-bp chemically synthesized polylinker with different restriction sites. The new polylinker is inserted in frame at the N-terminal end of the beta galactosidase lac Z fragment. The plasmid was named pLH1, the phage M13LH1.
Howard
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LA
Gene Expression Section, Amersham International plc, Bucks, United Kingdom.
Ortlepp
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SA
D001435:Bacteriophages / Q000235:genetics; D001483:Base Sequence; D001709:Biotechnology; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010957:Plasmids
D004247:DNA; D004262:DNA Restriction Enzymes