Definition of an Ets1 protein domain required for nuclear localization in cells and DNA-binding activity in vitro.
Ets1 and Ets2 are nuclear phosphoproteins which bind to DNA in vitro and share two domains of strong identity. Deletion analyses of each of these conserved regions in Ets1 demonstrated that integrity of the carboxy-terminal domain, also conserved in the more distantly related elk and erg gene products, is essential for both nuclear targeting and DNA-binding activity in vitro.
Boulukos
K E
KE
Institut National de la Santé de la Recherche Medicale U186/Centre National de la Recherche Scientifique UA 041160, Institut Pasteur, Lille, France.
Pognonec
P
P
Rabault
B
B
Begue
A
A
Ghysdael
J
J
D000818:Animals; D002460:Cell Line; D002872:Chromosome Deletion; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005822:Genetic Vectors; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D050783:Proto-Oncogene Proteins c-ets; D011519:Proto-Oncogenes; D013539:Simian virus 40 / Q000235:genetics; D014157:Transcription Factors; D014162:Transfection
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D014157:Transcription Factors; D011505:Protein-Tyrosine Kinases