title: Tunicamycin modulates binding of 125I-erythropoietin to Friend erythroleukemia cells.
abstract: The effects of tunicamycin and neuraminidase treatment on the specific binding of 125I-erythropoietin to a murine erythroleukemia cell clone, B8, were investigated. Neuraminidase treatment of B8 cells did not affect the specific binding of erythropoietin, but tunicamycin treatment caused a 2.5 to 4-fold increase in the amount of 125I-erythropoietin binding. Scatchard analysis of the binding data showed that the increase in the amount of binding resulted from increased affinity of the receptor. These results suggest that N-linked sugars of the erythropoietin receptor protein are involved in the interaction of erythropoietin with the cell-surface receptors on B8 cells.
authors.forename: H
authors.initials: H
authors.lastname: Fukamachi
authors.forename: A
authors.initials: A
authors.lastname: Tojo
authors.forename: T
authors.initials: T
authors.lastname: Saito
authors.forename: T
authors.initials: T
authors.lastname: Kitamura
authors.forename: A
authors.initials: A
authors.lastname: Urabe
authors.forename: F
authors.initials: F
authors.lastname: Takaku
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004921:Erythropoietin / Q000378:metabolism*; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism*; D051379:Mice; D011485:Protein Binding / Q000187:drug effects; D011956:Receptors, Cell Surface / Q000378:metabolism*; D017467:Receptors, Erythropoietin; D014415:Tunicamycin / Q000494:pharmacology*
chemical_list: D011956:Receptors, Cell Surface; D017467:Receptors, Erythropoietin; D014415:Tunicamycin; D004921:Erythropoietin