Absence of effect of angiotensin II on adenylate cyclase in rat proximal renal tubular basolateral membranes.
3H-Angiotensin II binding was studied in rat renal proximal tubular basolateral membranes. The characteristics of binding were found to be consistent with a receptor for angiotensin II at this site. Effects of angiotensin II on cAMP production were studied in the basolateral membranes. Angiotensin II had no effect on the time course of basal or parathyroid hormone stimulated cAMP production in basolateral membranes. Angiotensin II did not exhibit a concentration-dependent effect on cAMP production in basolateral membranes, either alone or in the presence of parathyroid hormone. The concentration-response curves of cAMP production in response to parathyroid hormone in basolateral membranes were similar in the presence or in the absence of angiotensin II. We conclude that adenylate cyclase production is not linked to the receptor for angiotensin II in rat renal proximal tubular basolateral membranes.
Lewis
N P
NP
Department of Pharmacology, University of Cambridge, UK.
Ferguson
D R
DR
D000067956:Adenylyl Cyclase Inhibitors; D000469:Alkaline Phosphatase / Q000378:metabolism; D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology; D002116:Calcitonin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology; D007687:Kidney Tubules, Proximal / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D008297:Male; D008566:Membranes / Q000187:drug effects / Q000378:metabolism; D010281:Parathyroid Hormone / Q000494:pharmacology; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D000067956:Adenylyl Cyclase Inhibitors; D010281:Parathyroid Hormone; D000804:Angiotensin II; D001127:Arginine Vasopressin; D002116:Calcitonin; D000242:Cyclic AMP; D000469:Alkaline Phosphatase; D010744:Phosphoric Monoester Hydrolases; D007545:Isoproterenol