Insulin does not activate a phosphoinositide-specific phospholipase C in adipocytes.
Phosphoinositide-specific phospholipase C (PI-PLC) activity was determined in homogenates of adipocytes treated with maximal concentrations of insulin. PI-PLC activity measured using exogenous [3H]phosphatidylinositol [( 3H]PI) and exogenous [3H]phosphatidylinositol 4,5-bisphosphate [( 3H]PIP2) was not altered by prior exposure of adipocytes to insulin. It was possible to see oxytocin-induced breakdown of phosphoinositides but no effect of insulin was seen in intact adipocytes.
Etindi
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Department of Biochemistry, University of Tennessee, Memphis 38163.
Fain
J N
JN
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D001647:Bile Acids and Salts / Q000502:physiology; D007328:Insulin / Q000502:physiology*; D010121:Oxytocin / Q000502:physiology; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D054801:Phosphoinositide Phospholipase C; D010727:Phosphoric Diester Hydrolases / Q000096:biosynthesis*; D051381:Rats; D010738:Type C Phospholipases / Q000096:biosynthesis*
D001647:Bile Acids and Salts; D007328:Insulin; D010121:Oxytocin; D010727:Phosphoric Diester Hydrolases; D010738:Type C Phospholipases; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase