title: Studies on the active site of pig plasma amine oxidase.
abstract: Amine oxidase from pig plasma (PPAO) has two bound Cu2+ ions and at least one pyrroloquinoline quinone (PQQ) moiety as cofactors. It is shown that recovery of activity by copper-depleted PPAO is linear with respect to added Cu2+ ions. Recovery of e.s.r. and optical spectral characteristics of active-site copper parallel the recovery of catalytic activity. These results are consistent with both Cu2+ ions contributing to catalysis. Further e.s.r. studies indicate that the two copper sites in PPAO, unlike those in amine oxidases from other sources, are chemically distinct. These comparative studies establish that non-identity of the Cu2+ ions in PPAO is not a requirement for amine oxidase activity. It is shown through the use of a new assay procedure that there are two molecules of PQQ bound per molecule of protein in PPAO; only the more reactive of these PQQ moieties is required for activity.
authors.affiliation: Department of Chemistry, University of Manchester, U.K.
authors.forename: D
authors.initials: D
authors.lastname: Collison
authors.forename: P F
authors.initials: PF
authors.lastname: Knowles
authors.forename: F E
authors.initials: FE
authors.lastname: Mabbs
authors.forename: F X
authors.initials: FX
authors.lastname: Rius
authors.forename: I
authors.initials: I
authors.lastname: Singh
authors.forename: D M
authors.initials: DM
authors.lastname: Dooley
authors.forename: C E
authors.initials: CE
authors.lastname: Cote
authors.forename: M
authors.initials: M
authors.lastname: McGuirl
mesh_terms: D006631:Amine Oxidase (Copper-Containing); D000818:Animals; D001051:Apoenzymes / Q000097:blood; D001665:Binding Sites; D003067:Coenzymes / Q000032:analysis; D004578:Electron Spin Resonance Spectroscopy; D007700:Kinetics; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000097:blood*; D045542:PQQ Cofactor; D011728:Pyridones / Q000494:pharmacology; D015363:Quinolones / Q000032:analysis / Q000494:pharmacology; D013053:Spectrophotometry; D013552:Swine; D013861:Thiocyanates / Q000494:pharmacology
chemical_list: D001051:Apoenzymes; D003067:Coenzymes; D011728:Pyridones; D015363:Quinolones; D013861:Thiocyanates; C062607:2-hydrazinopyridine; D045542:PQQ Cofactor; D006631:Amine Oxidase (Copper-Containing); D000587:Oxidoreductases Acting on CH-NH Group Donors