Neuropeptide Y inhibits vasoactive intestinal peptide- and dopamine-induced cyclic AMP formation in human Ewing's sarcoma WE-68 cells.
Neuropeptide Y (NPY) regulation of intracellular cyclic AMP accumulation was studied in human Ewing's sarcoma cell line, WE-68. NPY inhibited vasoactive intestinal peptide (VIP)- and dopamine-stimulated but not basal cyclic AMP formation. The peptide effect was rapid (less than 2 min), concentration-dependent with a half-maximal effective concentration (EC50) of 8 nM NPY, and maximal inhibition reaching 60-70% with 100 nM NPY. Prior exposure of WE-68 cells to pertussis toxin completely abolished the inhibitory action of NPY. It is concluded that NPY attenuates agonist-stimulated cyclic AMP formation in Ewing's sarcoma WE-68 cells, and may do so via the inhibitory guanine nucleotide regulatory protein of adenylate cyclase.
van Valen
F
F
Universitätskinderklinik, Abt. für Hämatologie und Onkologie, Düsseldorf, FRG.
Keck
E
E
Jürgens
H
H
D000242:Cyclic AMP / Q000037:antagonists & inhibitors* / Q000378:metabolism; D004298:Dopamine / Q000494:pharmacology*; D006801:Humans; D009478:Neuropeptide Y / Q000494:pharmacology*; D012512:Sarcoma, Ewing / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D014660:Vasoactive Intestinal Peptide / Q000037:antagonists & inhibitors*
D009478:Neuropeptide Y; D014660:Vasoactive Intestinal Peptide; D000242:Cyclic AMP; D004298:Dopamine