Purification of a p47 phosphoprotein from Xenopus laevis oocytes and identification as an in vivo and in vitro p34cdc2 substrate.
This paper describes the purification of a 47 kDa protein from Xenopus laevis oocytes that becomes phosphorylated when the oocytes undergo meiotic maturation. This protein (p47) is part of a high molecular mass complex containing at least two other proteins of molecular mass 30 and 36 kDa. This complex can be isolated from stage VI oocytes before maturation. We obtained a pattern for phosphopeptides in p47 phosphorylated in vivo very similar to that of the purified protein phosphorylated in vitro by p34cdc2 (a H1 kinase which is a component of the M-phase promoting factor) and [gamma-32P]ATP. Therefore, the purified p47, already described as a marker of MPF activity, is the first reported in vivo substrate for the cell division control kinase.
Mulner-Lorillon
O
O
Laboratoire de Physiologie de la Reproduction, INRA, UA CNRS 555, Université Pierre et Marie Curie, Paris, France.
Poulhe
R
R
Cormier
P
P
Labbe
J C
JC
Doree
M
M
Belle
R
R
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D016203:CDC2 Protein Kinase; D047388:Casein Kinases; D002918:Chymotrypsin / Q000378:metabolism; D000242:Cyclic AMP / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006133:Growth Substances / Q000378:metabolism*; D016200:Maturation-Promoting Factor; D008540:Meiosis; D008970:Molecular Weight; D009865:Oocytes / Q000032:analysis*; D010446:Peptide Fragments / Q000378:metabolism; D010750:Phosphoproteins / Q000302:isolation & purification* / Q000378:metabolism; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D012697:Serine Endopeptidases / Q000378:metabolism; D014982:Xenopus laevis
D006133:Growth Substances; D010446:Peptide Fragments; D010750:Phosphoproteins; D000255:Adenosine Triphosphate; D000242:Cyclic AMP; D011494:Protein Kinases; D047388:Casein Kinases; D016203:CDC2 Protein Kinase; D016200:Maturation-Promoting Factor; D012697:Serine Endopeptidases; D002918:Chymotrypsin; C028344:glutamyl endopeptidase