title: Intracellular localization of 99Tcm-d,l-HMPAO and 201Tl-DDC in rat brain.
abstract: The intracellular localization and relative distribution of 99Tcm-hexamethylpropyleneamine-oxime (99Tcm-d, l-HMPAO) and 201Tl-diethyldithiocarbamate (201Tl-DDC), which have been used to assess regional cerebral blood flow (rCBF) in man, were investigated in rat brain. 99Tcm-d, l-HMPAO was found attached mainly to cell organelles whilst 201Tl-DDC was localized mainly in free state in the cytosolic fraction. Isolation of neuronal and glial nuclei showed that there is higher uptake of 99Tcm-d, l-HMPAO in neuronal nuclei than in glial nuclei for all the different regions of the rat brain investigated. These data demonstrate a different subcellular localization of the two lipophilic agents studied. In view of their proposed clinical utility, this difference merits further investigation.
authors.affiliation: Institute of Nuclear Medicine, University College and Middlesex School of Medicine, London, UK.
authors.forename: D C
authors.initials: DC
authors.lastname: Costa
authors.forename: D
authors.initials: D
authors.lastname: Lui
authors.forename: A K
authors.initials: AK
authors.lastname: Sinha
authors.forename: P H
authors.initials: PH
authors.lastname: Jarritt
authors.forename: P J
authors.initials: PJ
authors.lastname: Ell
mesh_terms: D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging*; D004050:Ditiocarb; D005260:Female; D015609:Organotechnetium Compounds; D010091:Oximes; D011877:Radionuclide Imaging; D051381:Rats; D011919:Rats, Inbred Strains; D019690:Technetium Tc 99m Exametazime; D013794:Thallium Radioisotopes; D014018:Tissue Distribution
chemical_list: D015609:Organotechnetium Compounds; D010091:Oximes; D013794:Thallium Radioisotopes; D019690:Technetium Tc 99m Exametazime; D004050:Ditiocarb