Detection of swine pox and buffalo pox viruses in cell culture using a protein A-horseradish peroxidase conjugate.
Buffalo pox virus antigen was detected in Vero cells and swine pox virus antigen in the cytoplasm and nucleus of PK-15 and IB-RS-2 cells as early as 6 hr post infection (p.i.) by indirect immunoperoxidase technique using a Protein A-horseradish peroxidase (HRP) conjugate. The viral antigens localized in the cytoplasm of infected cells were the most prominent after 24 hr p.i.
Mohanty
P K
PK
Division of Biological Products, Indian Veterinary Research Institute, Izatnagar.
Verma
P C
PC
Rai
A
A
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002020:Buffaloes / Q000382:microbiology*; D002460:Cell Line; D006735:Horseradish Peroxidase; D007124:Immunoenzyme Techniques; D011212:Poxviridae / Q000302:isolation & purification*; D013205:Staphylococcal Protein A; D013552:Swine / Q000382:microbiology*
D000956:Antigens, Viral; D013205:Staphylococcal Protein A; D006735:Horseradish Peroxidase