Occurrence of human papillomavirus type 16 DNA sequences and c-myc oncogene alterations in uterine-cervix carcinoma.
Using genetic engineering and molecular biology techniques, we have examined sixteen human carcinomas in the uterine-cervix tumors (the most frequent tumor in México, representing 34% of malignant tumors in women), for the presence of Human Papillomavirus type 16 (HPV-16) DNA sequences and possible alterations of the cellular myc (c-myc) proto-oncogene. In this study we have analyzed cervical carcinomas from patient with clinical stage II. We detected in 31% of these samples, the presence of HPV-16 sequences (2-100 copies). In addition, an elevated amplification (up to 80-fold in one tumor) and/or rearrangement of the c-myc oncogene was detected in most tumors (more than 90% of the samples). These results suggest that either c-myc oncogene and/or HPV-16 could play an important role in the development of uterine-cervix carcinoma.
Ocádiz
R
R
Unidad de Oncología, Hospital General de México, SSA y Centro Médico La Raza-IMSS.
Sauceda
R
R
Salcedo
M
M
Ortega
V
V
Rodríguez
H
H
Gordillo
C
C
Chávez
P
P
Gariglio
P
P
D002277:Carcinoma / Q000235:genetics / Q000382:microbiology*; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000302:isolation & purification*; D005260:Female; D016259:Genes, myc; D006801:Humans; D027383:Papillomaviridae / Q000302:isolation & purification*; D011230:Precancerous Conditions / Q000235:genetics / Q000382:microbiology; D000090063:Proto-Oncogene Mas; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics; D014412:Tumor Virus Infections / Q000235:genetics / Q000382:microbiology; D002577:Uterine Cervical Diseases / Q000235:genetics / Q000382:microbiology; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000382:microbiology*
D015344:DNA Probes, HPV; D004279:DNA, Viral; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D016271:Proto-Oncogene Proteins c-myc