Direct extraction and assay of collagenase from human osteoarthritic articular cartilage.
A method has been developed for the direct extraction of collagenase from small quantities (5 mg) of human osteoarthritic articular cartilage. The enzyme, which was not detected in normal cartilage, was entirely in a latent form and demonstrated typical properties of mammalian collagenase after activation by trypsin.
Blanckaert
A
A
Laboratoire de Pathologie Ostéo-articulaire, CHU Rangueil, Toulouse, France.
Mazières
B
B
Eeckhout
Y
Y
Vaes
G
G
D000818:Animals; D002358:Cartilage, Articular / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D051379:Mice; D003012:Microbial Collagenase / Q000032:analysis*; D017830:Octoxynol; D010003:Osteoarthritis / Q000201:enzymology*; D011092:Polyethylene Glycols / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology
D011092:Polyethylene Glycols; D017830:Octoxynol; D014357:Trypsin; D003012:Microbial Collagenase