title: Rescue of excitation-contraction coupling in dysgenic muscle by addition of fibroblasts in vitro.
abstract: Muscular dysgenesis (mdg) in mice causes the failure of excitation-contraction (E-C) coupling in skeletal muscle. Cultured dysgenic muscle fails to contract upon depolarization, lacks typical muscle ultrastructure, including normal triads, and lacks functional voltage-dependent slow calcium channels. We show that normal rodent fibroblasts and 3T3 fibroblasts "rescue" dysgenic myotubes, reestablishing contractions (i.e., E-C coupling), normal ultrastructure, and functional slow calcium channels. These results support the finding that the expression of the slow calcium channel is affected in the mdg mutation and that this protein is essential for E-C coupling. Additionally, fibroblast rescue provides a system for examining the mechanisms of heterotypic cellular influence on cell function.
authors.affiliation: Laboratoire de Neurobiologie Cellulaire, Université de Bordeaux II, Talence, France.
authors.forename: P
authors.initials: P
authors.lastname: Courbin
authors.forename: J
authors.initials: J
authors.lastname: Koenig
authors.forename: A
authors.initials: A
authors.lastname: Ressouches
authors.forename: K G
authors.initials: KG
authors.lastname: Beam
authors.forename: J A
authors.initials: JA
authors.lastname: Powell
mesh_terms: D000200:Action Potentials; D000818:Animals; D015220:Calcium Channels / Q000502:physiology; D002450:Cell Communication; D002478:Cells, Cultured; D005347:Fibroblasts / Q000502:physiology*; D051379:Mice; D008817:Mice, Mutant Strains; D009119:Muscle Contraction; D009137:Muscular Dystrophy, Animal / Q000473:pathology*; D010673:Pheochromocytoma / Q000473:pathology; D051381:Rats; D012518:Sarcomeres / Q000648:ultrastructure; D013116:Spinal Cord / Q000166:cytology; D014407:Tumor Cells, Cultured / Q000502:physiology
chemical_list: D015220:Calcium Channels