Down-regulation of testicular aromatization in the horse.
A single i.m. injection of testosterone (750 mg of testosterone bexahydrobenzoate) or i.v. injection of human chorionic gonadotrophin (hCG) (10,000 IU) was given to geldings and stallions. Levels of unconjugated and conjugated (after solvolysis) androgens and estrogens were measured in blood and urine samples taken daily from the day of injection (D0) to the tenth day post-injection (D10). In the stallion, both treatments resulted in a sharp increase of plasma estrogens, which peaked one day before the androgen levels. Our results confirmed the testicular localization of a potent aromatase, which is able to aromatize androgens from endogenous as well as exogenous origin into conjugated estrogens. The very similar patterns of estrogen increase following testosterone or hCG administration suggest that the estrogen rise induced by hCG results at least partly from increased availability of testosterone. The abrupt drop in plasma estrogen levels cannot be explained by a lack of substrate, since two successive androgen injections did not succeed in maintaining the high estrogen levels. Since estrogens were unable to inhibit the aromatase activity in vitro, the drop in estrogen levels suggests a down-regulation of the aromatase synthesis.
Zwain
I
I
Laboratoire de Biochimie, UA CNRS 609 Universite, Caen, France.
Gaillard
J L
JL
Dintinger
T
T
Silberzahn
P
P
D000728:Androgens / Q000378:metabolism / Q000502:physiology; D000818:Animals; D047072:Aromatase Inhibitors; D006063:Chorionic Gonadotropin / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D004967:Estrogens / Q000378:metabolism*; D004966:Estrogens, Conjugated (USP) / Q000378:metabolism; D006736:Horses; D008297:Male; D009919:Orchiectomy; D013737:Testis / Q000187:drug effects / Q000378:metabolism*; D013739:Testosterone / Q000008:administration & dosage* / Q000502:physiology
D000728:Androgens; D047072:Aromatase Inhibitors; D006063:Chorionic Gonadotropin; D004967:Estrogens; D004966:Estrogens, Conjugated (USP); D013739:Testosterone