A simplified and rapid test for acetylator phenotyping by use of the peak height ratio of two urinary caffeine metabolites.
We describe a simplified liquid-chromatographic test in which acetylator phenotype is determined by measuring the peak height ratio of two urinary caffeine metabolites, 5-acetylamino-6-formylamino-3-methyluracil and 1-methylxanthine. We applied this test to determine the acetylator phenotypes of 52 subjects who regularly drink coffee, tea, or caffeinated beverages. Also, we determined the acetylator phenotypes of these subjects according to a well-established sulfasalazine test, which yielded identical results. We established the reproducibility of the described test by determining the acetylator phenotypes of 10 additional subjects on two different days separated by a period of two to five weeks. Of the 52 subjects examined by both tests, 40 (76.9%) were classified as slow acetylators, which agrees well with the percentage reported elsewhere for 297 similar subjects from the Saudi population.
el-Yazigi
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Biological and Medical Research Department, King Faisal Specialist Hospital, Saudi Arabia.
Chaleby
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Martin
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D000107:Acetylation; D000123:Acetyltransferases / Q000378:metabolism*; D000328:Adult; D000368:Aged; D001191:Arylamine N-Acetyltransferase / Q000235:genetics / Q000378:metabolism*; D002110:Caffeine / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D012460:Sulfasalazine; D014498:Uracil / Q000031:analogs & derivatives / Q000652:urine; D014970:Xanthines / Q000652:urine
D014970:Xanthines; C008514:methylxanthine; D002110:Caffeine; D012460:Sulfasalazine; D014498:Uracil; C036692:5-acetylamino-6-formylamino-3-methyluracil; D000123:Acetyltransferases; D001191:Arylamine N-Acetyltransferase