title: Nucleotide sequence of the Vibrio alginolyticus glnA region.
abstract: The nucleotide sequence of a 4 kb fragment containing the Vibrio alginolyticus glnA, ntrB and ntrC genes was determined. The upstream region of the glnA gene contained tandem promoters. The upstream promoter resembled the consensus sequence for Escherichia coli sigma 70 promoters whereas the presumptive downstream promoter showed homology with nitrogen regulated promoters. Four putative NRI binding sites were located between the tandem promoters. The ntrB gene was preceded by a single presumptive NRI binding site. The ntrC gene was located 45 base pairs downstream from the ntrB gene. The V. alginolyticus ntrB and ntrC genes were able to complement ntrB, ntrC deletions in E. coli.
authors.affiliation: Department of Microbiology, University of Cape Town, Rondebosch, South Africa.
authors.forename: R
authors.initials: R
authors.lastname: Maharaj
authors.forename: E
authors.initials: E
authors.lastname: Rumbak
authors.forename: W A
authors.initials: WA
authors.lastname: Jones
authors.forename: S M
authors.initials: SM
authors.lastname: Robb
authors.forename: F T
authors.initials: FT
authors.lastname: Robb
authors.forename: D R
authors.initials: DR
authors.lastname: Woods
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics*; D005974:Glutamate-Ammonia Ligase / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014733:Vibrio / Q000201:enzymology / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D015719:Single-Strand Specific DNA and RNA Endonucleases; D005974:Glutamate-Ammonia Ligase