title: Synthesis and benzodiazepine receptor affinities of rigid analogues of 3-carboxy-beta-carbolines: demonstration that the benzodiazepine receptor recognizes preferentially the s-cis conformation of the 3-carboxy group.
abstract: 1H-Indolo[3',2':4,5]pyrido[3,2-b]-2-penten-5-olide (6) and 1H,5H-indolo[3',2'-c]-6,7-dihydro-2-pyridone (7), rigid analogues of methyl 4-ethyl-beta-carboline-3-carboxylate (8) and N-methyl-4-ethyl-beta-carboline-3-carboxamide (9), respectively, were synthesized and their in vitro binding affinities to the central type benzodiazepine receptors were compared. The IC50 values of 6 and 8 were approximately equivalent (42 and 27 nM, respectively). The amide derivative 9, for which theoretical energy calculations indicate that the s-trans carbonyl conformation is the preferred one, displayed very low affinity (IC50 greater than 10(4) nM). However, when the carbonyl group of 9 was forced to adopt the s-cis conformation as in lactam 7, binding to the benzodiazepine receptor was largely restored (IC50 = 150 nM), indicating that the s-cis carboxy conformation at C-3 of beta-carbolines is preferentially recognized by this receptor. In vivo, compound 6 showed neither convulsant, proconvulsant, nor anticonvulsant activity in mice. Moreover, 6 did not antagonize methyl beta-carboline-3-carboxylate induced convulsions in mice. This lack of activity of 6 was attributed to its inability to cross the blood-brain barrier since no significant displacement of [3H]Ro 15-1788 from mouse brain benzodiazepine receptors by 6 could be observed in vivo.
authors.affiliation: Institut de Chimie des Substances Naturelles Centre National de la Recherche Scientifique, Gif-sur-Yvette, France.
authors.forename: G
authors.initials: G
authors.lastname: Dorey
authors.forename: G
authors.initials: G
authors.lastname: Poissonnet
authors.forename: M C
authors.initials: MC
authors.lastname: Potier
authors.forename: L
authors.initials: L
authors.lastname: Prado de Carvalho
authors.forename: P
authors.initials: P
authors.lastname: Venault
authors.forename: G
authors.initials: G
authors.lastname: Chapouthier
authors.forename: J
authors.initials: J
authors.lastname: Rossier
authors.forename: P
authors.initials: P
authors.lastname: Potier
authors.forename: R H
authors.initials: RH
authors.lastname: Dodd
mesh_terms: D000818:Animals; D002243:Carbolines / Q000138:chemical synthesis* / Q000378:metabolism; D055598:Chemical Phenomena; D002621:Chemistry; D066298:In Vitro Techniques; D051379:Mice; D011963:Receptors, GABA-A / Q000378:metabolism*; D013237:Stereoisomerism; D013329:Structure-Activity Relationship
chemical_list: D002243:Carbolines; D011963:Receptors, GABA-A