title: The effect of endogenous essential and nonessential fatty acids on the uptake and subsequent agonist-induced release of arachidonate.
abstract: We have demonstrated that the uptake and agonist-induced release of a pulse of arachidonate are influenced by the size and composition of preexisting endogenous fatty acid pools. EFD-1 cells, an essential fatty acid-deficient mouse fibrosarcoma cell line, were incubated with radiolabeled (14C or 3H] arachidonate, linoleate, eicosapentaenoate (EPA), palmitate, or oleate in concentrations of 0-33 microM for 24 h. After 24 h, the cells were pulsed with 0.67 microM radiolabeled (3H or 14C, opposite first label) arachidonate for 15 min and then stimulated with 10 microM bradykinin for 4 min. Because EFD-1 cells contain no endogenous essential fatty acids, we were able to create essential fatty acid-repleted cells for which the specific activity of the newly constructed endogenous essential fatty acid pool was known. Loading the endogenous pool with the essential fatty acids arachidonate, eicosapentaenoate, or linoleate (15-20 nmol of fatty acid incorporated/10(6) cells) decreased the uptake of a pulse of arachidonate from 200 to 100 pmol/10(6) cells but had no effect on palmitate uptake. The percent of arachidonate incorporated during the pulse which was released upon agonist stimulation increased 2-fold (4-8%) as the endogenous pool of essential fatty acids was increased from 0 to 15-20 nmol/10(6) cells. This 8% release was at least 3-fold greater than the percent release from the various endogenous essential fatty acid pools. In contrast, loading the endogenous pool with the nonessential fatty acids oleate or palmitate to more than 2-3 times their preexisting cellular level had no effect on the uptake of an arachidonate pulse. Like the essential fatty acids, increasing endogenous oleate increased (by 2-fold) the percent release of arachidonate incorporated during the pulse, whereas endogenous palmitate had no effect on subsequent agonist-induced release from this arachidonate pool. These studies show that preexisting pools of essential and nonessential fatty acids exert different effects on the uptake and subsequent releasability of a pulse of arachidonate.
authors.affiliation: Department of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania, Philadelphia 19104.
authors.forename: E E
authors.initials: EE
authors.lastname: Furth
authors.forename: V
authors.initials: V
authors.lastname: Hurtubise
authors.forename: M A
authors.initials: MA
authors.lastname: Schott
authors.forename: M
authors.initials: M
authors.lastname: Laposata
mesh_terms: D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism* / Q000494:pharmacology; D001920:Bradykinin / Q000494:pharmacology; D002250:Carbon Radioisotopes; D015118:Eicosapentaenoic Acid / Q000494:pharmacology; D005227:Fatty Acids / Q000494:pharmacology*; D005228:Fatty Acids, Essential / Q000494:pharmacology*; D005354:Fibrosarcoma; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000494:pharmacology; D051379:Mice; D019301:Oleic Acid; D009829:Oleic Acids / Q000494:pharmacology; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000494:pharmacology; D014316:Tritium; D014407:Tumor Cells, Cultured
chemical_list: D001095:Arachidonic Acids; D002250:Carbon Radioisotopes; D005227:Fatty Acids; D005228:Fatty Acids, Essential; D008041:Linoleic Acids; D009829:Oleic Acids; D010169:Palmitic Acids; D014316:Tritium; D016718:Arachidonic Acid; D019301:Oleic Acid; D019308:Palmitic Acid; D019787:Linoleic Acid; D015118:Eicosapentaenoic Acid; D001920:Bradykinin