Characteristics of in vitro passaged cells derived from a rat transplantable malignant fibrous histiocytoma.
Morphologic and functional characteristics were investigated on in vitro passaged cells (MT-P) derived from a rat transplantable malignant fibrous histiocytoma (MFH-MT). There were spindle, polygonal, and giant cell types in MT-P. Ultrastructurally, the polygonal and giant cells had the abundant cytoplasm with many lysosomes and processes, whereas the spindle cells possessed smooth cell surface and a small number of lysosomes in their cytoplasm. Immunorosette formation for Fc- and C3-surface receptors and phagocytic activity were demonstrated in 10-20% of MT-P. MT-P were positive for acid phosphatase, nonspecific esterase and alkaline phosphatase. Chromosomes counted in 100 MT-P ranged from 32 to 100 with two peaks of 64 and 76. Tumors induced in syngeneic rats by inoculating MT-P showed variable histologic patterns. They were composed partly of histiocytic cells arranged in a compact sheet. Fibroblastic cells often arranged in a storiform pattern or were supported by myxoid matrix. Osteosarcoma-like structures were occasionally found in the tumors. These results suggest that MFH-MT is heterogeneous, although some cells constituting the tumors have histiocytic markers.
Yamate
J
J
Tajima
M
M
Togo
M
M
Shibuya
N
N
Shibuya
K
K
Kudow
S
S
D000818:Animals; D015726:Giant Cells / Q000648:ultrastructure*; D018219:Histiocytoma, Benign Fibrous / Q000276:immunology / Q000648:ultrastructure*; D007621:Karyotyping; D008297:Male; D008854:Microscopy, Electron; D009368:Neoplasm Transplantation; D010587:Phagocytosis; D051381:Rats; D011916:Rats, Inbred F344; D012397:Rosette Formation; D014407:Tumor Cells, Cultured