title: Infectious cDNA clones of the DA strain of Theiler's murine encephalomyelitis virus.
abstract: The DA strain and other members of the TO subgroup of Theiler's murine encephalomyelitis viruses cause a persistent demyelinating infection, whereas the GDVII strain and other GDVII subgroup strains cause an acute lethal polioencephalomyelitis. We generated an infectious DA cDNA clone inserted into a transcription vector. Virus derived from transfection of transcripts produced a demyelinating disease indistinguishable from that of wild-type virus. The infectious clone provides a critical reagent for the production of interstrain recombinant viruses to help identify genetic loci responsible for the biological activities of the strains.
authors.affiliation: Department of Neurology, University of Chicago Pritzker School of Medicine, Illinois 60637.
authors.forename: R P
authors.initials: RP
authors.lastname: Roos
authors.forename: S
authors.initials: S
authors.lastname: Stein
authors.forename: Y
authors.initials: Y
authors.lastname: Ohara
authors.forename: J L
authors.initials: JL
authors.lastname: Fu
authors.forename: B L
authors.initials: BL
authors.lastname: Semler
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D004770:Enterovirus / Q000235:genetics*; D005822:Genetic Vectors; D007739:L Cells / Q000382:microbiology; D004680:Maus Elberfeld virus / Q000235:genetics* / Q000472:pathogenicity; D051379:Mice; D008969:Molecular Sequence Data; D010957:Plasmids; D015183:Restriction Mapping; D013116:Spinal Cord / Q000382:microbiology / Q000473:pathology; D014158:Transcription, Genetic; D014162:Transfection; D014774:Virulence
chemical_list: D004279:DNA, Viral