A novel primase-free form of murine DNA polymerase alpha induced by infection with minute virus of mice.
Two species of DNA polymerase alpha free of primase activity were identified in extracts of Ehrlich mouse cells that had been infected with minute virus of mice. Primase-free forms of DNA polymerase alpha eluted with 150 and 180 mM NaCl during ion-exchange chromatography on DEAE-cellulose columns, exhibited sedimentation coefficients of 11 S and 8.2 S, respectively, and were inhibited by aphidicolin, N2-(p-n-butylphenyl)-9-(2-deoxy-beta-D-ribofuranosyl)guanine 5'-triphosphate, and 2-(p-n-butylanilino)-9-(2-deoxy-beta-D-ribofuranosyl)adenine 5'-triphosphate. The ratio of primase-free DNA polymerase alpha to the DNA polymerase alpha-primase complex increased from 1.5 to greater than 100 during the course of infection, and free primase was produced during the MVM replicative cycle.
Ho
T F
TF
Cancer Research Laboratory, University of Western Ontario, London, Canada.
Gupta
J S
JS
Faust
E A
EA
D000818:Animals; D016590:Aphidicolin; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology* / Q000382:microbiology; D002460:Cell Line; D002852:Chromatography, Ion Exchange / Q000379:methods; D004257:DNA Polymerase II / Q000235:genetics / Q000302:isolation & purification*; D019915:DNA Primase; D004279:DNA, Viral / Q000378:metabolism; D004224:Diterpenes / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D051379:Mice; D008915:Minute Virus of Mice / Q000235:genetics; D009154:Mutation; D010322:Parvoviridae Infections / Q000201:enzymology* / Q000235:genetics; D012316:RNA Nucleotidyltransferases / Q000032:analysis*; D014779:Virus Replication
D004279:DNA, Viral; D004224:Diterpenes; D016590:Aphidicolin; D019915:DNA Primase; D012316:RNA Nucleotidyltransferases; D004257:DNA Polymerase II