3H-ouabain binding sites in porcine skeletal muscle as influenced by environmental temperature and energy intake.
The influence of environmental temperature and energy intake on 3H-ouabain binding sites in skeletal muscle has been investigated in young growing pigs at 8 weeks of age. Animals lived for several weeks at 35 or 10 degrees C on a high (H) or low (L) level of energy intake. The four treatment groups were thus: 35H, 35L, 10H and 10L. The total number of 3H-ouabain binding sites (Bmax) in longissimus dorsi muscle (mean values +/- SEM) were 221 +/- 66, 214 +/- 61, 350 +/- 76 and 486 +/- 114 pmol/g wet weight for the 35H, 35L, 10H and 10L groups respectively. Bmax was significantly greater in those living in the cold than the warm (P less than 0.001). Moreover, at 10 degrees C energy intake had a significant effect, with Bmax being greater in the 10L than the 10H group (P less than 0.005). Level of energy intake had no influence on Bmax at 35 degrees C. The apparent dissociation constant was not affected by either temperature or intake. The elevated Bmax and hence the increase in number of Na+,K+-pumping sites in the cold is probably related to increased muscular activity associated with shivering. However, thyroid status also influences the number of Na+,K+-pumping sites and this may have been a contributory factor in the present study. In addition, the elevated Bmax suggests a greater potential for non-shivering thermogenesis associated with increased Na+,K+-ATPase concentration in the cold. Differences in relative stage of development between the four groups may help to explain the results for Bmax in relation to level of energy intake.
Dauncey
M J
MJ
AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, Great Britain.
Burton
K A
KA
D000818:Animals; D001665:Binding Sites; D004734:Energy Metabolism; D008297:Male; D009132:Muscles / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D010042:Ouabain / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013552:Swine; D013696:Temperature; D013997:Time Factors; D014316:Tritium / Q000378:metabolism
D014316:Tritium; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase