title: Expression of Epstein-Barr virus nuclear antigens 3, 4, and 6 are altered in cell lines containing B-type virus.
abstract: A high proportion of HIV-positive sera were found to react with 130- and 180-kDa antigens which were present in the Jijoye cell line. The majority of the HIV-positive sera which detected these antigens also contained antibodies to Epstein-Barr virus (EBV) nuclear antigen 2B (EBNA2B) suggesting a relationship between B-type EBV strains and the expression of the 130K/180K antigens. Cell lines were established by infection of B lymphocytes with different A- and B-type strains of EBV. Incubation of these lines with sera from individuals infected with either A-type or B-type EBV strains demonstrated that the 130K and 180K antigens were only expressed by cell lines containing B-type virus. Sera from individuals infected with A-type EBV did not react with the 180K antigen in any cell lines but could detect EBNAs 3, 4, and 6 antigens in the A-type cell lines. Restriction enzyme analysis of the BamHI E region of the EBV genome revealed marked differences between the A and B types of the virus. These results demonstrate that expression of antigens encoded from the BamHI E region of EBV (EBNAs 3, 4, and 6) are altered in cell lines transformed by B-type strains of EBV.
authors.affiliation: Queensland Institute of Medical Research, Herston, Brisbane, Australia.
authors.forename: T B
authors.initials: TB
authors.lastname: Sculley
authors.forename: A
authors.initials: A
authors.lastname: Apolloni
authors.forename: R
authors.initials: R
authors.lastname: Stumm
authors.forename: D J
authors.initials: DJ
authors.lastname: Moss
authors.forename: N
authors.initials: N
authors.lastname: Mueller-Lantczh
authors.forename: I S
authors.initials: IS
authors.lastname: Misko
authors.forename: D A
authors.initials: DA
authors.lastname: Cooper
mesh_terms: D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D015153:Blotting, Western; D002460:Cell Line; D002472:Cell Transformation, Viral; D019309:Epstein-Barr Virus Nuclear Antigens; D006679:HIV Seropositivity / Q000276:immunology; D006566:Herpesviridae Infections / Q000276:immunology / Q000382:microbiology; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000276:immunology*; D006801:Humans; D008970:Molecular Weight; D015183:Restriction Mapping
chemical_list: D000956:Antigens, Viral; D019309:Epstein-Barr Virus Nuclear Antigens