[Presence of rotavirus and adenovirus in fecal samples of children with gastroenteritis, in the city of Goyania].
In an attempt to detect rotavirus and adenovirus prevalence among other enteropathogens (bacteria and parasites) in diarrhoea, three hundred fecal samples originating from children living in Goiânia city (Goiás state, Brazil) were analysed. Rotavirus was found to be the only pathogen in 47 cases, and associated with other infectious agents in 21 cases. 97.0% positive samples of rotavirus showed an electrophoretic pattern characteristic of subgroup II. Adenovirus was found in 7 cases, and associated with other microorganisms in 1 case. Three methods were applied for virological analyses: enzyme immunoassay for rotavirus and adenovirus (EIARA), polyacrylamide gel electrophoresis (PAGE) and immunoelectron microscopy (IEM). The concordance among the three methods was 92.8%, PAGE and EIARA agreed in 95.8%, and IEM and EIARA agreed in 100.0%.
Cardoso
D D
DD
de Brito
W M
WM
Martins
R M
RM
Kitajima
E W
EW
Souza
M P
MP
Barbosa
A J
AJ
de Oliveira
S A
SA
Rascopi
S B
SB
D000208:Acute Disease; D000260:Adenoviruses, Human / Q000302:isolation & purification*; D001938:Brazil; D002648:Child; D002675:Child, Preschool; D005243:Feces / Q000382:microbiology*; D005759:Gastroenteritis / Q000382:microbiology* / Q000469:parasitology; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D012401:Rotavirus / Q000302:isolation & purification*
Ocorrência de rotavírus e adenovírus em amostras fecais de crianças com gastrenterite, na cidade de Goiânia.