title: Short-term regulation of glycolysis by vasoactive intestinal peptide in epithelial cells isolated from rat small intestine.
abstract: In epithelial cells isolated from rat small intestine, we have studied the influence of vasoactive intestinal peptide (VIP), a neurotransmitter which markedly increases enterocyte cyclic AMP, and of two cyclic AMP analogues (8-bromo cyclic AMP and N6,2'-O-dibutyryl cyclic AMP) on the rate of glycolysis, fructose 2,6-bisphosphate concentration and 6-phosphofructo-2-kinase activity, as well as on the rate of 3-O-methyl-D-[14C]glucose uptake. Our results show that, without affecting the rate of 3-O-methyl-D-[14C]glucose accumulation, VIP and cyclic AMP analogues were able to inhibit glucose consumption and L-lactate formation by isolated rat enterocytes. These effects occurred parallel to a significant decrease in the cellular concentration of fructose 2,6-bisphosphate and to a partial inactivation of 6-phosphofructo-2-kinase. These findings support the hypothesis that VIP inhibits glycolysis in rat enterocytes through a cyclic AMP-dependent mechanism.
authors.affiliation: Servicio de Gastroenterología, Clínica Puerta de Hierro, Facultad de Medicina, Universidad Autónoma de Madrid, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Rossi
authors.forename: L
authors.initials: L
authors.lastname: Monge
authors.forename: J E
authors.initials: JE
authors.lastname: Feliu
mesh_terms: D019325:3-O-Methylglucose; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002469:Cell Separation; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D005635:Fructosediphosphates / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects*; D007421:Intestine, Small / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008757:Methylglucosides / Q000378:metabolism; D051381:Rats; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology*
chemical_list: D005635:Fructosediphosphates; D008757:Methylglucosides; D019325:3-O-Methylglucose; D014660:Vasoactive Intestinal Peptide; D003994:Bucladesine; C027652:fructose 2,6-diphosphate