title: Localization of the active type I DNA topoisomerase gene on human chromosome 20q11.2-13.1, and two pseudogenes on chromosomes 1q23-24 and 22q11.2-13.1.
abstract: Different subfragments of a cDNA coding for DNA topoisomerase I were used as probes to determine the chromosomal localization of topoisomerase I sequences in human cells. Southern blotting of restricted DNA from a panel of rodent-human somatic cell hybrids revealed the localization of the complete gene on chromosome 20 and the presence of two truncated topoisomerase I pseudogene sequences on chromosomes 1 and 22. In situ chromosome hybridization experiments confirmed these results showing the location of the complete gene on band q11.2-13.1 of chromosome 20, and the location of the pseudogene sequences on band q23-24 of chromosome 1 and q11.2-13.1 of chromosome 22.
authors.affiliation: Fakultät für Biologie der Universität, Konstanz, Federal Republic of Germany.
authors.forename: N
authors.initials: N
authors.lastname: Kunze
authors.forename: G C
authors.initials: GC
authors.lastname: Yang
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Jiang
authors.forename: H
authors.initials: H
authors.lastname: Hameister
authors.forename: S
authors.initials: S
authors.lastname: Adolph
authors.forename: K H
authors.initials: KH
authors.lastname: Wiedorn
authors.forename: A
authors.initials: A
authors.lastname: Richter
authors.forename: R
authors.initials: R
authors.lastname: Knippers
mesh_terms: D015139:Blotting, Southern; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D002890:Chromosomes, Human, Pair 20; D002892:Chromosomes, Human, Pair 22; D015342:DNA Probes; D004264:DNA Topoisomerases, Type I / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D005796:Genes; D006367:HeLa Cells / Q000201:enzymology; D006801:Humans; D009693:Nucleic Acid Hybridization; D011544:Pseudogenes
chemical_list: D015342:DNA Probes; D004273:DNA, Neoplasm; D004264:DNA Topoisomerases, Type I