Adrenergic regulation of the uncoupling protein expression in foetal rat brown adipocytes in primary culture.
The adrenergic and T3 modulation of UCP expression in non-proliferative foetal brown adipocyte primary cultures were studied. The UCP in the cultured cells was determined by immunological detection of the protein and by quantification of the mitochondrial GDP-binding. Our results showed a relative increase of 65-75% in UCP levels and 60-80% in the mitochondrial GDP-binding capacity under beta-adrenergic stimulatory conditions, while neither alpha 1-adrenergic agonists nor T3 showed an effect.
Porras
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Departamento de Bioquímica y Biología Molecular, Facultad de Farmacia, Ciudad Universitaria, Madrid, Spain.
Fernández
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Benito
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D002001:Adipose Tissue, Brown / Q000196:embryology* / Q000378:metabolism; D000818:Animals; D002352:Carrier Proteins; D002478:Cells, Cultured; D004734:Energy Metabolism; D006153:Guanosine Diphosphate / Q000378:metabolism; D066298:In Vitro Techniques; D007473:Ion Channels; D007545:Isoproterenol / Q000494:pharmacology; D008565:Membrane Proteins / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins; D051381:Rats; D011943:Receptors, Adrenergic, beta / Q000502:physiology; D014284:Triiodothyronine / Q000494:pharmacology; D000071256:Uncoupling Protein 1
D002352:Carrier Proteins; D007473:Ion Channels; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D011943:Receptors, Adrenergic, beta; D000071256:Uncoupling Protein 1; D014284:Triiodothyronine; D006153:Guanosine Diphosphate; D007545:Isoproterenol