Ca2+-dependent and cAMP-dependent control of nicotinic acetylcholine receptor phosphorylation in muscle cells.
Mouse BC3H1 myocytes were incubated with 32Pi before acetylcholine receptors were solubilized, immunoprecipitated, and subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis. More than 90% of the 32P found in the receptor was bound to the delta subunit. Two phosphorylation sites in this subunit were resolved by reverse phase high performance liquid chromatography after exhaustive proteolysis of the protein with trypsin. Sites 1 and 2 were phosphorylated to approximately the same level in control cells. The divalent cation ionophore, A23187, increased 32P in site 1 by 40%, but did not affect the 32P content of site 2. In contrast, isoproterenol increased 32P in site 2 by more than 60%, while increasing 32P in site 1 by only 20%. When dephosphorylated receptor was incubated with [gamma-32P]ATP and the catalytic subunit of cAMP-dependent protein kinase, the delta subunit was phosphorylated to a maximal level of 1.6 phosphates/subunit. Approximately half of the phosphate went into site 2, with the remainder going into a site not phosphorylated in cells. The alpha subunit was phosphorylated more slowly, but phosphorylation of both alpha and delta subunits was blocked by the heat-stable protein inhibitor of cAMP-dependent protein kinase. Phosphorylation of the receptor was also observed with preparations of phosphorylase kinase. In this case phosphorylation occurred in the beta subunit and site 1 of the delta subunit, neither of which were phosphorylated by cAMP-dependent protein kinase. The rate of receptor phosphorylation by phosphorylase kinase was slow relative to that catalyzed by cAMP-dependent protein kinase. Therefore, it can not yet be concluded that phosphorylase kinase phosphorylates the beta subunit and the delta subunit site 1 in cells. However, the results strongly support the hypothesis that phosphorylation by cAMP-dependent protein kinase accounts for phosphorylation of the alpha subunit and the delta subunit site 2 in response to elevations in cAMP.
Smith
M M
MM
Department of Pharmacology, Washington University School of Medicine, St. Louis, Missouri 63110.
Merlie
J P
JP
Lawrence
J C
JC
D000596:Amino Acids / Q000302:isolation & purification; D000818:Animals; D002118:Calcium / Q000502:physiology*; D002851:Chromatography, High Pressure Liquid; D000242:Cyclic AMP / Q000502:physiology*; D051379:Mice; D009132:Muscles / Q000378:metabolism*; D010748:Phosphopeptides; D010761:Phosphorus Radioisotopes; D010764:Phosphorylase Kinase / Q000502:physiology; D010766:Phosphorylation; D011233:Precipitin Tests; D011494:Protein Kinases / Q000502:physiology; D011978:Receptors, Nicotinic / Q000378:metabolism*
D000596:Amino Acids; D010748:Phosphopeptides; D010761:Phosphorus Radioisotopes; D011978:Receptors, Nicotinic; D000242:Cyclic AMP; D011494:Protein Kinases; D010764:Phosphorylase Kinase; D002118:Calcium