Typing of human rhinoviruses based on sequence variations in the 5' non-coding region.
Unambiguous assignment of restriction enzyme patterns to six individual serotypes of human rhinovirus was accomplished after amplification of a 380 bp DNA fragment derived from the 5' non-coding region. This was possible even though serotypes 1A and 1B and serotypes 2 and 49 differed only at 10 and 15 positions respectively. The method utilizes the conserved and variable components of this part of the genome and provides the basis for a simple and rapid method for typing of human rhinoviruses.
Torgersen
H
H
Institut für Biochemie, Universität Wien, Vienna, Austria.
Skern
T
T
Blaas
D
D
D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D005784:Gene Amplification; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics; D015183:Restriction Mapping; D012229:Rhinovirus / Q000145:classification* / Q000235:genetics; D012703:Serotyping
D004279:DNA, Viral; D012367:RNA, Viral