Binding of Escherichia coli S fimbriae to cultured human endothelial cells.
The binding of Escherichia coli adhesins to human umbilical vein endothelial cells was studied by a cell monolayer enzyme-linked immunosorbent assay. S fimbriae displayed a concentration-dependent and saturable binding to the endothelial cells which was mediated by their sialylgalactoside-specific lectin activity. P fimbriae exhibited only low binding, and type 1 fimbriae exhibited no binding to these cells.
Parkkinen
J
J
Department of Gynecology and Obstetrics, Helsinki University Central Hospital, Finland.
Ristimäki
A
A
Westerlund
B
B
D018830:Adhesins, Escherichia coli; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis / Q000502:physiology; D001790:Blood Physiological Phenomena; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000032:analysis / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000472:pathogenicity / Q000502:physiology*; D010861:Fimbriae, Bacterial / Q000032:analysis / Q000502:physiology*; D006801:Humans; D012795:Sialoglycoproteins / Q000097:blood; D014471:Umbilical Veins; D014774:Virulence
D018830:Adhesins, Escherichia coli; D001425:Bacterial Outer Membrane Proteins; D012795:Sialoglycoproteins