Immunochemical studies for the presence of P-450 HFLa, a form of cytochrome P-450 in human fetal livers in human hepatocellular carcinoma cells.
Immunohistochemical localization of P-450 HFLa, a form of cytochrome P-450 in human fetal livers was investigated in human hepatocellular carcinoma. The cytoplasm of carcinoma cells positively reacted with anti-P-450 HFLa antibodies. It was found that the carcinoma cells showing a pseudoglandular pattern or poorly differentiated appearance exhibited a weaker reactivity with anti-P-450 HFLa antibodies than did relatively differentiated carcinoma cells. In the case of hepatoblastoma, the polygonal or round-shaped tumor cells which differentiated into epithelial structure exhibited a positive reaction with anti-P-450 HFLa antibodies, whereas the spindle-shaped tumor cells which showed a sarcomatous appearance did not react with the antibodies.
Kitada
M
M
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Tsukidate
K
K
Takeuchi
J
J
Taneda
M
M
Komori
M
M
Ohi
H
H
Itahashi
K
K
Kamataki
T
T
D000328:Adult; D006528:Carcinoma, Hepatocellular / Q000201:enzymology*; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis*; D005333:Fetus / Q000201:enzymology; D006801:Humans; D007124:Immunoenzyme Techniques; D008099:Liver / Q000196:embryology / Q000201:enzymology*; D008113:Liver Neoplasms / Q000201:enzymology*
D003577:Cytochrome P-450 Enzyme System