Double labeling of cytochrome oxidase and gamma-aminobutyric acid in central nervous system neurons of adult cats.
The relationship between the levels of cytochrome oxidase and gamma-aminobutyric acid (GABA) was investigated within single neurons by double labeling the 2 markers in the same section. Double staining was equally effective when immunogold-silver staining of GABA was followed by indirect immunoperoxidase labeling of cytochrome oxidase, or when cytochrome oxidase histochemistry was followed by immunogold-silver staining of GABA. Neurons in the perigeniculate nucleus (PGN) and basket cell terminals in the cerebellum were GABA positive and rich in cytochrome oxidase. Interneurons of the lateral geniculate nucleus (LGN) as well as stellate and Golgi cells of the cerebellum were GABA-rich but poor in cytochrome oxidase. These results demonstrate that there is no consistent relationship between the levels of cytochrome oxidase and GABA in neurons.
Luo
X G
XG
Department of Anatomy and Cellular Biology, Medical College of Wisconsin, Milwaukee 53226.
Hevner
R F
RF
Wong-Riley
M T
MT
D000818:Animals; D002415:Cats; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000378:metabolism*; D005829:Geniculate Bodies / Q000166:cytology / Q000378:metabolism*; D006651:Histocytochemistry; D007150:Immunohistochemistry; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D005680:gamma-Aminobutyric Acid; D003576:Electron Transport Complex IV