title: The Mos proto-oncogene maps near the centromere on mouse chromosome 4.
abstract: The Mos proto-oncogene, the cellular homolog of the transforming gene of Moloney murine sarcoma virus, was originally assigned to mouse chromosome 4 using independent panels of mouse/hamster somatic cell hybrids. By in situ hybridization to metaphase chromosomes and standard genetic backcrosses, we have confirmed this assignment and determined that Mos maps near the centromere in a region devoid of other markers. We have also identified a restriction fragment length polymorphism (RFLP) that defines two alleles of the Mos locus in selected inbred strains of laboratory mice. Using the RFLP, we determined the strain distribution pattern for the Mos gene in three sets of recombinant inbred strains and in five strains congenic for histocompatibility antigen genes localized on chromosome 4. These results establish Mos as a useful marker in a poorly characterized region of the mouse genome. In addition, these results will facilitate the genetic analysis of the Mos locus.
authors.affiliation: NCI-Frederick Cancer Research Facility, BRI-Basic Research Program, Maryland 21701.
authors.forename: F
authors.initials: F
authors.lastname: Propst
authors.forename: G F
authors.initials: GF
authors.lastname: Vande Woude
authors.forename: N A
authors.initials: NA
authors.lastname: Jenkins
authors.forename: N G
authors.initials: NG
authors.lastname: Copeland
authors.forename: B K
authors.initials: BK
authors.lastname: Lee
authors.forename: P A
authors.initials: PA
authors.lastname: Hunt
authors.forename: E M
authors.initials: EM
authors.lastname: Eicher
mesh_terms: D000483:Alleles; D000818:Animals; D002503:Centromere; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004247:DNA / Q000235:genetics; D005260:Female; D005819:Genetic Markers / Q000032:analysis; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009693:Nucleic Acid Hybridization; D017232:Oncogene Proteins v-mos; D012150:Polymorphism, Restriction Fragment Length; D011519:Proto-Oncogenes; D011995:Recombination, Genetic; D012191:Retroviridae Proteins / Q000235:genetics*; D014178:Translocation, Genetic
chemical_list: D005819:Genetic Markers; D017232:Oncogene Proteins v-mos; D012191:Retroviridae Proteins; D004247:DNA