Genomic distribution of copia-like transposable elements in somatic tissues and during development of Drosophila melanogaster.
The genomic distribution of elements of the copia, 412, B 104, mdg 1, mdg 4 and 1731 transposon families was compared by the Southern technique in DNA preparations extracted from brains, salivary glands and adult flies of two related Drosophila lines. The copia, 412 and mdg 1 sequences were also probed in DNA from sperm, embryos, and 1st and 2nd instar larvae. The homogeneity of the patterns observed shows that somatic transposition is unlikely to occur frequently. A correlation between mobility and the euchromatic or heterochromatic location of transposable elements is discussed. In addition, an explanation of the variable band intensities of transposable elements in Southern autoradiographs is proposed.
Di Franco
C
C
Centro per lo Studio degli Acidi Nucleici C.N.R., Università La Sapienza, Roma, Italy.
Pisano
C
C
Dimitri
P
P
Gigliotti
S
S
Junakovic
N
N
D000818:Animals; D015139:Blotting, Southern; D001921:Brain; D004251:DNA Transposable Elements / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D015698:Genomic Library; D007814:Larva / Q000235:genetics*; D008297:Male; D009693:Nucleic Acid Hybridization; D009928:Organ Specificity / Q000235:genetics; D012469:Salivary Glands / Q000032:analysis; D013094:Spermatozoa / Q000032:analysis
D004251:DNA Transposable Elements