Erythrocyte sodium transport in dialyzed uremic patients.
To investigate the status of the Na+ concentration and ionic fluxes in red cells of human subjects with dialyzed chronic uremia, the authors measured the Na(+)-K+ pump activity as well as Na(+)-K+ cotransport (CoT), Na(+)-Li+ countertransport (CTT) and Na+ passive permeability in erythrocytes from 37 normal subjects and 23 chronic uremic patients receiving maintenance hemodialysis. The mean intracellular Na+ concentration [Na+]i value in the pre-dialytic group was significantly lower than that in control subjects (p less than .0001), but tended to recover to the normal value of [Na+]i in the post-dialytic group. The mean intracellular K+ concentration value in the post-dialytic group was significantly higher than that of the control group (p less than .001), but not significantly different from that of the pre-dialytic group. It was found that the Na(+)-K+ pump activity of erythrocytes in the pre- and post-dialytic groups markedly decreased over that of the normal control group with statistical significance (p less than .0001, respectively). The Na(+)-K+ pump activity in the post-dialytic group, however, tended to recover, but not significantly. The rate constant for ouabain-sensitive Na+ efflux in the post-dialytic group was significantly decreased over that of the normal controls (p less than .05). The authors observed a significant decrease of the Na+ CoT value (p less than .001 respectively) and rate constant for Na+ CoT (p less than .05, respectively) in the patients with pre- and post-dialytic uremia vs. that of normal subjects.(ABSTRACT TRUNCATED AT 250 WORDS)
Yoon
Y S
YS
Kim
S Y
SY
Koo
W S
WS
Choi
E J
EJ
Chang
Y S
YS
Bang
B K
BK
Moon
H R
HR
D000328:Adult; D000368:Aged; D001693:Biological Transport, Active; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D006801:Humans; D008094:Lithium / Q000097:blood; D008297:Male; D008875:Middle Aged; D011188:Potassium / Q000097:blood*; D015221:Potassium Channels / Q000378:metabolism; D006435:Renal Dialysis; D012964:Sodium / Q000097:blood*; D015222:Sodium Channels / Q000378:metabolism; D014511:Uremia / Q000097:blood* / Q000628:therapy
D015221:Potassium Channels; D015222:Sodium Channels; D008094:Lithium; D012964:Sodium; D011188:Potassium