Demonstration of bovine virus diarrhoea virus antigen in formalin fixed, paraffin embedded tissue using a streptavidin/biotin technique.
The detection of bovine virus diarrhoea virus (BVDV) antigen in sections from formalin fixed, paraffin embedded tissue is described. Pre-digestion of the sections with 0.02 per cent protease XIV for 18 hours at 4 degrees C is necessary to unmask formalin fixed antigen. A hyperimmune antiserum prepared in a pig, using a combination of BVDV and hog cholera virus inoculations, linked to a biotinylated anti-pig/streptavidin peroxidase detection system demonstrated antigen in a wide range of tissues from cases of mucosal disease and persistently viraemic animals. The inclusion of a monoclonal anti-pig immunoglobulin linked to a biotinylated anti-mouse/streptavidin peroxidase detection system greatly reduced non-specific staining.
Allan
G M
GM
Veterinary Research Laboratories, Stormont, Belfast.
McNulty
M S
MS
Bryson
D
D
Mackie
D
D
Platten
M
M
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D001426:Bacterial Proteins; D001710:Biotin; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000276:immunology / Q000473:pathology*; D002417:Cattle; D002418:Cattle Diseases / Q000473:pathology*; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology*; D010576:Pestivirus / Q000276:immunology*; D019809:Streptavidin
D000956:Antigens, Viral; D001426:Bacterial Proteins; D001710:Biotin; D019809:Streptavidin