Identification of a major GTP-binding protein in bovine aortic smooth muscle membranes as smg p21, a GTP-binding protein having the same effector domain as ras p21s.
At least two GTP-binding proteins (G proteins) with Mr values of about 20,000 were extracted from bovine aortic smooth muscle membranes by sodium cholate. The most abundant G protein (22K G) was purified to near homogeneity by successive column chromatographies of Ultrogel AcA-44, phenyl-Sepharose CL-4B, hydroxyapatite and Mono Q HR5/5. 22K G showed kinetic and physical properties very similar to those of smg p21, a G protein recently isolated from bovine brain and human platelet membranes, having the same effector domain as ras p21s. Moreover, 22K G was recognized specifically by the anti-smg p21 antibody. These results indicate that the major G protein in bovine aortic smooth muscle membranes is smg p21.
Kawata
M
M
Department of Internal Medicine, Kobe University School of Medicine, Japan.
Kawahara
Y
Y
Araki
S
S
Sunako
M
M
Tsuda
T
T
Fukuzaki
H
H
Mizoguchi
A
A
Takai
Y
Y
D000818:Animals; D001011:Aorta / Q000378:metabolism*; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D002845:Chromatography; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D017886:Durapatite; D019204:GTP-Binding Proteins / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism; D006882:Hydroxyapatites; D015151:Immunoblotting; D008970:Molecular Weight; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000276:immunology; D016283:Proto-Oncogene Proteins p21(ras); D013873:Thionucleotides / Q000378:metabolism
D006882:Hydroxyapatites; D011518:Proto-Oncogene Proteins; D013873:Thionucleotides; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate; D017886:Durapatite; D019204:GTP-Binding Proteins; D016283:Proto-Oncogene Proteins p21(ras)