Nutritional requirements of papillomavirus-transformed mouse cells and an uninfected parent line in serum-free culture.
A serum-free culture system was used to compare the nutritional requirements of mouse mammary cells transformed by bovine papillomavirus type 1 (ID13 cells) and the uninfected parent line (C127 cells). The serum-free, chemically defined medium used for this study was an MCDB 151-based medium (MCDB 151+S+I), supplemented with epidermal growth factor, transferrin, hydrocortisone, ethanolamine, phosphoethanolamine, retinoic acid, trace metals, and insulin. Proliferation of either cell type in serum-free culture required the addition of 250 micrograms/ml of insulin. ID13 cells have a doubling time of greater than 96 h in MCDB 151+S+I, whereas C127 cells have a doubling time of 60 h. This is in sharp contrast to the growth characteristics of the two cell types in 10% fetal bovine serum, where doubling times for the ID13 and C127 cells are 24 and 30 h, respectively. Culture of the cells in a serum-free medium has therefore revealed that the papillomavirus-transformed cells have more stringent growth requirement than the uninfected parent line.
Di Lorenzo
T P
TP
Department of Biology, Hofstra University, Hempstead, New York 11550.
De Maro
J A
JA
Pumo
D E
DE
D000818:Animals; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral / Q000187:drug effects / Q000502:physiology; D003470:Culture Media / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004983:Ethanolamines / Q000494:pharmacology; D005260:Female; D006854:Hydrocortisone / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D008321:Mammary Glands, Animal / Q000166:cytology* / Q000473:pathology / Q000502:physiology; D008670:Metals / Q000494:pharmacology; D051379:Mice; D009751:Nutritional Requirements; D027383:Papillomaviridae; D014168:Transferrin / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology
D003470:Culture Media; D004983:Ethanolamines; D007328:Insulin; D008670:Metals; D014168:Transferrin; D014212:Tretinoin; D004815:Epidermal Growth Factor; D006854:Hydrocortisone