Isolation and characterisation of transposon-induced mutants of Erwinia carotovora subsp. atroseptica exhibiting reduced virulence.
The blackleg pathogen Erwinia carotovora subsp. atroseptica (Eca) causes an economically important disease of potatoes. We selected a genetically amenable Eca strain for the genetic analysis of virulence. Tn5 mutagenesis was used to generate nine mutants which exhibited reduced virulence (Rvi-) of strain SCRI1043. Following physiological characterisation, mutants were divided into three classes: (1) auxotrophs; (2) extracellular enzyme mutants; and (3) a growth rate mutant. The isolation of these Rvi- mutants has allowed us to consider some factors that affect Eca virulence.
Hinton
J C
JC
Department of Biological Sciences, University of Warwick, Coventry, England.
Sidebotham
J M
JM
Hyman
L J
LJ
Pérombelon
M C
MC
Salmond
G P
GP
D015169:Colony Count, Microbial; D003227:Conjugation, Genetic; D003470:Culture Media; D004251:DNA Transposable Elements; D004885:Erwinia / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism / Q000472:pathogenicity; D005838:Genotype; D009154:Mutation; D010641:Phenotype; D010935:Plant Diseases; D010957:Plasmids; D011198:Solanum tuberosum / Q000382:microbiology; D014161:Transduction, Genetic; D014774:Virulence
D003470:Culture Media; D004251:DNA Transposable Elements