title: Early pre-B-cell transformation induced by the v-fms oncogene in long-term mouse bone marrow cultures.
abstract: Murine long-term bone marrow cultures that support B-lymphoid-cell development were infected with a helper-free retrovirus containing the v-fms oncogene. Infection of B-lymphoid cultures resulted in the rapid clonal outgrowth of early pre-B cells, which grew to high cell densities on stromal cell feeder layers, expressed v-fms-coded glycoproteins, and underwent immunoglobulin heavy-chain gene rearrangements. Late-passage cultures gave rise to factor-independent variants that proliferated in the absence of feeder layers, developed resistance to hydrocortisone, and became tumorigenic in syngeneic mice. The v-fms oncogene therefore recapitulates known effects of the v-abl and bcr-abl oncogenes on B-lineage cells. The ability of v-fms to induce transformation of early pre-B cells in vitro underscores the capacity of oncogenic mutants of the colony-stimulating factor-1 receptor to function outside the mononuclear phagocyte lineage.
authors.affiliation: Howard Hughes Medical Institute, Department of Tumor Cell Biology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105.
authors.forename: G V
authors.initials: GV
authors.lastname: Borzillo
authors.forename: C J
authors.initials: CJ
authors.lastname: Sherr
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D002454:Cell Differentiation; D002455:Cell Division / Q000187:drug effects; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D006133:Growth Substances / Q000494:pharmacology; D051379:Mice; D016281:Oncogene Protein gp140(v-fms); D009857:Oncogenes; D010641:Phenotype; D012191:Retroviridae Proteins / Q000235:genetics; D005253:Sarcoma Viruses, Feline / Q000235:genetics; D013256:Steroids / Q000494:pharmacology
chemical_list: D006133:Growth Substances; D016281:Oncogene Protein gp140(v-fms); D012191:Retroviridae Proteins; D013256:Steroids