title: DNA probe for the identification of Histoplasma capsulatum.
abstract: A 1.85-kilobase HindIII nuclear DNA probe from Histoplasma capsulatum G217B detected polymorphic restriction fragments within whole-cell DNA from different clinical isolates of H. capsulatum, consistent with the previous system of classification. The probe failed to hybridize to DNA from Blastomyces dermatitidis, Candida spp., Saccharomyces cerevisiae, Sepedonium chrysospermum, and Chrysosporium keratinophilum under low-stringency conditions and therefore may have value as a diagnostic reagent to identify H. capsulatum.
authors.affiliation: Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110.
authors.forename: E J
authors.initials: EJ
authors.lastname: Keath
authors.forename: E D
authors.initials: ED
authors.lastname: Spitzer
authors.forename: A A
authors.initials: AA
authors.lastname: Painter
authors.forename: S J
authors.initials: SJ
authors.lastname: Travis
authors.forename: G S
authors.initials: GS
authors.lastname: Kobayashi
authors.forename: G
authors.initials: G
authors.lastname: Medoff
mesh_terms: D015342:DNA Probes; D004271:DNA, Fungal / Q000235:genetics; D015247:Deoxyribonuclease HindIII; D006658:Histoplasma / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D015342:DNA Probes; D004271:DNA, Fungal; D015247:Deoxyribonuclease HindIII