Variability in gold bead density in cells. Quantitative immunocytochemistry.
Variability in gold bead distribution between individual cells was demonstrated in both pituitary melanotrophic cells immunocytochemically reacted for ACTH and in neurohypophysial terminals reacted for oxytocin-neurophysin. Gold beads were confined to the secretory granules compartment of both tissues. Density of gold beads in melanotrophic cells reacted for ACTH varied from 100-480 gold beads/microns 2. A much narrower range of gold beads distribution (460-900 gold beads/microns 2) was observed in axons of the neurohypophysis reacted with anti-oxytocin-neurophysin. These results indicate that the labeling density varies from cell to cell (as well as axon terminals) within morphologically homogeneous populations. Thus, it may reflect certain physiological differences between cells. A suggestion is being made that mean gold bead density coefficient of variation should be calculated by comparison between individual cells.
Hammel
I
I
Department of Pathology, Sackler School o Medicine, Tel-Aviv University, Israel.
Elmalek
M
M
Castel
M
M
Kalina
M
M
D000324:Adrenocorticotropic Hormone / Q000032:analysis; D000818:Animals; D002452:Cell Count; D002454:Cell Differentiation; D003594:Cytoplasmic Granules / Q000032:analysis; D006046:Gold / Q000032:analysis*; D007150:Immunohistochemistry / Q000379:methods*; D008854:Microscopy, Electron; D009481:Neurophysins / Q000032:analysis; D010121:Oxytocin / Q000032:analysis; D010316:Particle Size; D010902:Pituitary Gland / Q000032:analysis / Q000378:metabolism*; D010904:Pituitary Gland, Posterior / Q000032:analysis / Q000378:metabolism*; D013223:Statistics as Topic
D009481:Neurophysins; D010121:Oxytocin; D006046:Gold; D000324:Adrenocorticotropic Hormone