Restriction of poliovirus RNA translation in a human monocytic cell line.
The infection of the human monocytic cell line U-937 by poliovirus was characterized by a low level of virus production and a slow progression of the cytopathic effect. Infection took place in greater than 99% of the cells as revealed by a limiting dilution assay. No viral protein synthesis was evident in the infected U-937 cells when analyzed by polyacrylamide gel electrophoresis. However, a low level of poliovirus RNA translation was detected by immunofluorescence analysis using a mixture of polyclonal antisera against non-structural proteins. Although there was only a low level of viral protein synthesis, a gradual accumulation of viral mRNA took place in U-937 cells as revealed by RNA blot analysis. Similar results were obtained when the erythroleukemic cell line K-562 was used as a host cell for poliovirus. RNA extracted from infected U-937 cells was efficiently translated in rabbit reticulocyte extracts giving rise to a pattern of viral polypeptides similar to that detected when virion-purified RNA was the template used for the in vitro translation assay, suggesting that the poliovirus RNA present in infected U-937 cells is functional. The existence in U-937 cells of a discriminatory mechanism which differentially interferes with poliovirus RNA translation is discussed.
López-Guerrero
J A
JA
Centro de Biología Molecular, Universidad Autónoma, Madrid, Spain.
Carrasco
L
L
Martínez-Abarca
F
F
Fresno
M
M
Alonso
M A
MA
D002460:Cell Line; D002472:Cell Transformation, Viral; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008715:Methionine / Q000378:metabolism; D009000:Monocytes; D017955:Poliovirus / Q000235:genetics*; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000235:genetics*; D011865:Radioisotope Dilution Technique; D013462:Sulfur Radioisotopes; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification
D012367:RNA, Viral; D013462:Sulfur Radioisotopes; D014764:Viral Proteins; D008715:Methionine