Labeling antibodies with copper radionuclides using N-4-nitrobenzyl-5-(4-carboxyphenyl)-10,15,20-tris(4-sulfophenyl) porphine.
Antibody conjugates labeled with copper-64 and -67 (64Cu and 67Cu) were prepared using the porphyrin chelator N-4-nitrobenzyl-5-(4-carboxyphenyl)-10,15,20-tris(4-sulfophenyl) porphine (N-bzHCS3P). N-bzHCS3P was chosen because it has only one carboxylate group available for activation and coupling to antibody. The conjugates were characterized with respect to (1) the location of the porphyrin on the antibody, (2) the retention of immunoreactivity, and (3) the serum stability of the amide bond linking porphyrin to antibody. These studies showed that porphyrin attachment on the antibody surface is random. The conjugates exhibited high retention of immunoreactivity and reasonable serum stability for potential application in nuclear medicine.
Roberts
J C
JC
Medical Radioisotopes Research Program, Los Alamos National Laboratory, NM 87545.
Newmyer
S L
SL
Mercer-Smith
J A
JA
Schreyer
S A
SA
Lavallee
D K
DK
D000906:Antibodies; D001666:Binding Sites, Antibody; D002614:Chelating Agents; D003301:Copper Radioisotopes; D004355:Drug Stability; D006801:Humans; D007120:Immunochemistry; D066298:In Vitro Techniques; D011166:Porphyrins; D011854:Radiochemistry
D000906:Antibodies; D002614:Chelating Agents; D003301:Copper Radioisotopes; D011166:Porphyrins; C062668:N-4-nitrobenzyl-5-(4-carboxyphenyl)-10,15,20-tris(4-sulfophenyl)porphine