title: The fission yeast dis2+ gene required for chromosome disjoining encodes one of two putative type 1 protein phosphatases.
abstract: S. pombe dis mutants block mitotic chromosome disjunction in a manner reminiscent of aneuploidy formation, and belong to three distinct genes, dis1-dis3. We cloned two independent genomic DNAs that complemented both the cold-sensitive and caffeine-hypersensitive phenotype of dis2-11. These genes, dis2+ and a suppressor sds21+, encode proteins (calculated MW 37,000) with similar predicted amino acid sequences. dis2+ and sds21+ have overlapping functions, and disruptants are lethal only when both genes are disrupted. The gene products identified by anti-dis2 serum are enriched in nuclei. By hybridization, we obtained two cDNA clones from mouse and one genomic clone from S. cerevisiae; the latter complements S. pombe dis2-11. These dis2+ and similar polypeptides of yeasts and mouse are found to be highly homologous (75%-90% identical) to rabbit protein phosphatase 1. The implications of these findings are discussed with regard to mitotic control.
authors.affiliation: Department of Biophysics, Faculty of Science, Kyoto University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ohkura
authors.forename: N
authors.initials: N
authors.lastname: Kinoshita
authors.forename: S
authors.initials: S
authors.lastname: Miyatani
authors.forename: T
authors.initials: T
authors.lastname: Toda
authors.forename: M
authors.initials: M
authors.lastname: Yanagida
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002110:Caffeine / Q000494:pharmacology; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003080:Cold Temperature; D004271:DNA, Fungal / Q000235:genetics; D005455:Fluorescent Antibody Technique; D005656:Fungal Proteins / Q000235:genetics; D005800:Genes, Fungal; D005804:Genes, Lethal; D005816:Genetic Complementation Test; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009630:Nondisjunction, Genetic; D010749:Phosphoprotein Phosphatases / Q000235:genetics* / Q000276:immunology; D054645:Protein Phosphatase 1; D012441:Saccharomyces cerevisiae / Q000235:genetics; D004718:Saccharomycetales / Q000235:genetics*; D012568:Schizosaccharomyces / Q000235:genetics*
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; D002110:Caffeine; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1