Eclipse products of poliovirus after cold-synchronized infection of HeLa cells.
The particles derived from radioactively labeled poliovirus, in cold-synchronized infection of HeLa cells, were studied. After temperatures shift-up, radioactivity was transferred rapidly from the infecting virions (160 S) to 135 S, and from there more slowly to 110 S, a previously unreported eclipse product. Both the 135 and 110 S particles contained RNA, lacked VP4, and were H-antigenic. In the 135 S particles, part of the VP1 complement was lost; in addition to this, the VP2 polypeptide of the 110 S particles was cleaved in situ. The formation of eclipse products was insensitive to cycloheximide. Arildone totally blocked the formation of 135 S particles, but not their further processing.
Everaert
L
L
Department of Microbiology and Hygiene, Vrije Universiteit Brussel, Belgium.
Vrijsen
R
R
Boeyé
A
A
D000956:Antigens, Viral / Q000032:analysis; D000998:Antiviral Agents; D002451:Cell Compartmentation; D002462:Cell Membrane / Q000378:metabolism; D003513:Cycloheximide / Q000494:pharmacology; D006367:HeLa Cells / Q000382:microbiology; D006801:Humans; D007659:Ketones / Q000494:pharmacology; D019814:Myristic Acid; D009227:Myristic Acids / Q000378:metabolism; D017955:Poliovirus / Q000254:growth & development* / Q000276:immunology / Q000648:ultrastructure; D012367:RNA, Viral / Q000032:analysis; D013997:Time Factors; D014461:Ultracentrifugation; D014764:Viral Proteins / Q000378:metabolism
D000956:Antigens, Viral; D000998:Antiviral Agents; D007659:Ketones; D009227:Myristic Acids; D012367:RNA, Viral; D014764:Viral Proteins; D019814:Myristic Acid; C020706:arildone; D003513:Cycloheximide