title: [Study of the inhibition of neutral phosphatase from Pseudomonadaceae by derivatives of its substrates].
abstract: The inhibition of neutral phosphatase isolated from the bacteria of the Pseudomonadaceae family by various fragments of the enzyme-hydrolyzed R-O-PO3H2 substrates, inorganic orthophosphate (KH2PO4) and its analogs as well as by adenine, adenosine, alcohols, sugars and amino acids, was studied. It was demonstrated that among other compounds tested only the orthophosphoric acid anions (H2PO4-) exhibit the properties of strong associative inhibitors (K1Vi = 4.35.10(-6)M of the enzyme. The pH dependence of the Michaelis constant [pKm0 = f(pH)] and the inhibition constant for phosphatase by potassium orthophosphate [pK1Vi(KH2PO4) = f(pH)] was studied. The presence in the enzyme active center of a carboxylic (pK = 4.3 +/- 0.1) (presumably, glutamine) and an imidazole (pK = 7.15 +/- 0.1) amino acid residues was postulated. The data obtained were compared to those for neutral, alkaline and acid phosphatases.
authors.forename: V I
authors.initials: VI
authors.lastname: Krupianko
authors.forename: A I
authors.initials: AI
authors.lastname: Severin
authors.forename: O A
authors.initials: OA
authors.lastname: Stepnaia
authors.forename: A G
authors.initials: AG
authors.lastname: Kozlovskiĭ
authors.forename: I S
authors.initials: IS
authors.lastname: Kulaev
mesh_terms: D000438:Alcohols / Q000494:pharmacology; D000596:Amino Acids / Q000494:pharmacology; D002241:Carbohydrates / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D009705:Nucleosides / Q000494:pharmacology; D010710:Phosphates / Q000494:pharmacology*; D010744:Phosphoric Monoester Hydrolases / Q000037:antagonists & inhibitors*; D011188:Potassium / Q000494:pharmacology*; D017680:Potassium Compounds; D011548:Pseudomonadaceae / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D000438:Alcohols; D000596:Amino Acids; D002241:Carbohydrates; D009705:Nucleosides; D010710:Phosphates; D017680:Potassium Compounds; C013216:potassium phosphate; D010744:Phosphoric Monoester Hydrolases; D011188:Potassium
vernacular_title: Issledovanie ingibirovaniia neĭtral'noĭ fosfatazy iz Pseudomonadaceae proizvodnymi ee substratov.