ELISA for detection of antibodies to a type D retrovirus, SRV-W.
An ELISA was developed to detect antibodies to a Type D retrovirus, SRV-W. The interpretation of the ELISA results were based on: 1) Comparisons of known antibody positive (to a closely related type D retrovirus) and negative serum samples, 2) the ability of ELISA reactivity to be absorbed with a Type D virus but not a mock virus preparation, and 3) analysis by a Western Blot assay as an alterative way to identify antibody to the Type D retrovirus.
Schultz
K T
KT
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison.
Thomas
C
C
Toohey
K
K
Curro
T
T
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D008251:Macaca / Q000276:immunology*; D008253:Macaca mulatta / Q000276:immunology*; D008992:Monkey Diseases / Q000276:immunology; D012192:Retroviridae Infections / Q000276:immunology / Q000662:veterinary; D015302:Simian Immunodeficiency Virus / Q000276:immunology* / Q000302:isolation & purification
D000914:Antibodies, Viral