Microinjection of endogenous and exogenous proteins into primary cultures of rat hepatocytes and the degradation of the injected proteins.
1. A method to microinject proteins into cells through packaging proteins to erythrocyte ghosts (erythrocyte-mediated microinjection) was modified partially in order to apply the method to primary cultures of rat hepatocytes. 2. Degradation of the microinjected proteins was examined employing the improved method. The mean half-life of the injected endogenous liver protein was 20 hr. The data suggested that the injected proteins are degraded through both lysosomal and non-lysosomal proteolytic pathways probably depending on their structure. 3. The present method to microinject exogenous proteins into primary cultures of rat hepatocytes can be employed usefully for the investigations of protein metabolism in liver.
Takenaka
A
A
Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo, Japan.
Tayama
T
T
Takahashi
S I
SI
Kato
H
H
Noguchi
T
T
Naito
H
H
D000818:Animals; D002458:Cell Fractionation; D002459:Cell Fusion; D002478:Cells, Cultured; D002850:Chromatography, Gel; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D004906:Erythrocyte Count; D004910:Erythrocyte Membrane / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism; D008297:Male; D008845:Microinjections; D009379:Neoplasms, Muscle Tissue / Q000378:metabolism; D015388:Organelles / Q000378:metabolism; D011480:Protease Inhibitors / Q000494:pharmacology; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012259:Ribonuclease, Pancreatic / Q000378:metabolism / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000378:metabolism / Q000494:pharmacology; D014508:Urea / Q000096:biosynthesis
D003470:Culture Media; D011480:Protease Inhibitors; D011506:Proteins; D012710:Serum Albumin, Bovine; D014508:Urea; D004247:DNA; D012259:Ribonuclease, Pancreatic