Effects of H-7 are not exclusively mediated through protein kinase C or the cyclic nucleotide-dependent kinases.
Culturing murine T cell tumor lines in the presence of the protein kinase inhibitor H-7 for 4 days led to their dependence on H-7 for maximal constitutive proliferation. Withdrawal of H-7 from H-7-conditioned cells led to inhibition of proliferation and cell death. The mechanism underlying this H-7 dependence does not appear to be related to clonal selection or to effects on protein kinase C or the cyclic nucleotide-dependent kinases. This suggests that all the effects of the widely used H-7 may not be completely understood, and that H-7 may be useful in the dissection of the complex patterns of growth regulation in T cell malignancies.
Love
J T
JT
Department of Medicine, University of Connecticut School of Medicine, Farmington 06032.
Padula
S J
SJ
Lingenheld
E G
EG
Amin
J K
JK
Sgroi
D C
DC
Wong
R L
RL
Sha'fi
R I
RI
Clark
R B
RB
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D007546:Isoquinolines / Q000494:pharmacology*; D051379:Mice; D008806:Mice, Inbred AKR; D010770:Phosphotransferases / Q000037:antagonists & inhibitors / Q000502:physiology*; D010879:Piperazines / Q000494:pharmacology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000502:physiology*; D013601:T-Lymphocytes / Q000201:enzymology / Q000473:pathology; D013945:Thymoma / Q000201:enzymology / Q000473:pathology; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000201:enzymology / Q000473:pathology
D007546:Isoquinolines; D010879:Piperazines; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D010770:Phosphotransferases; D011493:Protein Kinase C