Induction of Escherichia coli mastitis in cows fed selenium-deficient or selenium-supplemented diets.
Ten Holstein heifers were fed a selenium-deficient (SeD) diet (0.04 mg of Se/kg on a total ration dry-matter basis) 3 months before calving and throughout their first lactation. A selenium-supplemented (SeS) diet (2 mg of Se/head/d) was fed to a group of 10 heifers. In about the 14th week of lactation, the cows were challenge-exposed to Escherichia coli by administering 15 to 40 colony-forming units (CFU) into 1 mammary gland. Selenium concentration (microgram/ml) in blood around the time of challenge exposure was 0.033 +/- 0.002 (mean +/- SEM) in SeD and 0.132 +/- 0.006 in SeS cows. Infections were established in all challenge-exposed quarters. The frequency of quarter atrophy and agalactia, and reduction in whole-udder milk yield in the first 4 days after challenge exposure, were greater (P less than 0.05) in the SeD cows. Log10 peak bacterial concentrations in milk were higher (P less than 0.05) in SeD (7.63 +/- 0.34 CFU/ml) than in SeS cows (5.57 +/- 0.66 CFU/ml). Mean log bacterial concentration was significantly higher (P less than 0.05) from 12 to 20 hours after challenge exposure in SeD than in SeS cows. Duration of infection was significantly greater (P less than 0.05) in SeD (162.0 +/- 12.0) than in SeS cows (114.4 +/- 18.0 hours). Milk somatic cell counts increased significantly more slowly (P less than 0.05) in SeD than in SeS cows from 8 to 16 hours after challenge exposure. Ratios of milk somatic cells to bacteria in milk were significantly lower (P less than 0.05) in SeD than in SeS cows at 12 and 16 hours after challenge exposure.
Erskine
R J
RJ
Department of Veterinary Science, Pennsylvania State University, University Park 16802.
Eberhart
R J
RJ
Grasso
P J
PJ
Scholz
R W
RW
D000818:Animals; D002338:Carotenoids / Q000097:blood; D002417:Cattle; D003300:Copper / Q000097:blood; D004926:Escherichia coli / Q000254:growth & development; D004927:Escherichia coli Infections / Q000097:blood / Q000662:veterinary*; D005260:Female; D005978:Glutathione / Q000097:blood; D008414:Mastitis, Bovine / Q000097:blood / Q000382:microbiology*; D008892:Milk / Q000032:analysis / Q000378:metabolism; D009195:Peroxidase / Q000097:blood; D011247:Pregnancy; D012643:Selenium / Q000008:administration & dosage*; D013234:Stem Cells; D014810:Vitamin E / Q000097:blood; D019207:beta Carotene
D019207:beta Carotene; D014810:Vitamin E; D002338:Carotenoids; D003300:Copper; D009195:Peroxidase; D005978:Glutathione; D012643:Selenium