title: Fatty acid changes in liver choline and ethanolamine glycerophospholipids in aspirin-treated rats fed linoleate, gamma-linolenate and fish oil.
abstract: The effects of dietary linoleic acid, gamma-linolenic acid and marine fatty acids on the development of aspirin-induced gastric hemorrhage and the distribution of liver glycerophospholipid fatty acids in fat-deficient growing rats were studied. Aspirin (100 mg/day)-treated and nontreated rats were fed for 7 days, a mixed diet of 2.5% safflower oil and 7.5% hydrogenated coconut oil (SFO/HCO) or 7.5% fish oil (SFO/FO), or 2.5% gamma-linolenate concentrate and 7.5% fish oil (GLA/FO). Gastric hemorrhage was induced in animals by aspirin treatment to various extents. It was not affected by FO feeding, but was significantly alleviated by GLA feeding. Aspirin treatment reduced the proportions of 20:4n-6 in liver phosphatidylcholine. FO feeding (in SFO/FO and GLA/FO rats) further reduced the 20:4n-6 level and replaced it by n-3 fatty acids. GLA feeding, on the other hand, elevated the proportion of 20:4n-6. As a result, the reduction of 20:4n-6 by fish oil feeding, was less significant in GLA/FO rats than in SFO/FO rats. The degree of gastric hemorrhage appeared to relate negatively to the levels of 20:4n-6 in liver phosphatidylcholine, and to the sum of 20:4n-6 and 20:5n-3 when FO was included in the diet. It is suggested that long-chain polyunsaturated fatty acids (20:4n-6 and 20:5n-3) per se in addition to being precursors of prostaglandins, may also affect the development of gastric hemorrhage, possibly by modulating the permeability of cell membranes in the gastric mucosa.
authors.affiliation: Efamol Research Institute, Kentville, N.S., Canada.
authors.forename: Y S
authors.initials: YS
authors.lastname: Huang
authors.forename: Y
authors.initials: Y
authors.lastname: Watanabe
authors.forename: D F
authors.initials: DF
authors.lastname: Horrobin
authors.forename: V
authors.initials: V
authors.lastname: Simmons
mesh_terms: D000818:Animals; D001241:Aspirin / Q000633:toxicity*; D004041:Dietary Fats / Q000008:administration & dosage / Q000494:pharmacology; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D005395:Fish Oils / Q000378:metabolism / Q000494:pharmacology*; D006471:Gastrointestinal Hemorrhage / Q000139:chemically induced / Q000378:metabolism*; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000494:pharmacology; D008042:Linolenic Acids / Q000494:pharmacology; D008099:Liver / Q000378:metabolism*; D008297:Male; D010712:Phosphatidic Acids / Q000032:analysis*; D010713:Phosphatidylcholines / Q000032:analysis; D010714:Phosphatidylethanolamines / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D017965:gamma-Linolenic Acid
chemical_list: D004041:Dietary Fats; D005231:Fatty Acids, Unsaturated; D005395:Fish Oils; D008041:Linoleic Acids; D008042:Linolenic Acids; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D017965:gamma-Linolenic Acid; D019787:Linoleic Acid; D001241:Aspirin