title: IgG and IgA enhance the chrysotile-induced production of reactive oxygen metabolites by human polymorphonuclear leucocytes.
abstract: Chrysotile asbestos fibres induce a rapid generation of reactive oxygen metabolites by human polymorphonuclear leucocytes (PMNL) in vitro. This effect was markedly enhanced by the presence of 10-200 micrograms/ml of human gammaglobulin, purified polyclonal IgG, and monoclonal IgG and IgA myeloma proteins. Purified monoclonal IgD, IgM, kappa light chain proteins, and secretory IgA inhibited this chrysotile-induced response. No enhancing effect of IgG was observed when quartz dust or opsonized zymosan were used as stimulators of PMNL metabolism. The enhancing effect of IgG was shown to depend on opsonization of the asbestos fibre. We suggest that the IgG and IgA potentiating effect on the asbestos fibre-induced production of tissue-damaging reactive oxygen metabolites by inflammatory cells is dependent on a particle-specific binding of immunoglobulin to the fibre surface, with subsequent Fc receptor-mediated effects on cells. Such an interaction between certain immunoglobulins and asbestos may explain a number of in vivo phenomena in which immunological responses (hypergammaglobulinemia, circulating immune complexes, etc.) have been shown to relate to the progression of pulmonary asbestosis. The differences between various immunoglobulin classes and monoclonal immunoglobulins could represent an individual inflammation-modulating mechanism in the development of acute or chronic pulmonary asbestosis.
authors.affiliation: Institute of Occupational Health, Helsinki, Finland.
authors.forename: M
authors.initials: M
authors.lastname: Klockars
authors.forename: M
authors.initials: M
authors.lastname: Hedenborg
authors.forename: J
authors.initials: J
authors.lastname: Koistinen
authors.forename: T
authors.initials: T
authors.lastname: Isobe
mesh_terms: D001194:Asbestos / Q000276:immunology*; D017632:Asbestos, Serpentine; D002478:Cells, Cultured; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism*
chemical_list: D017632:Asbestos, Serpentine; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D013481:Superoxides; D001194:Asbestos