[A rapid radioenzyme method for determining dopamine and 3,4-dihydroxyphenylacetic acid in the basal ganglia of the rat brain].
A rapid and high sensitive radioenzymatic method of determination in the rat brain basal ganglia of dopamine (DA) and 3,4-dihydroxyphenylacetic acid (DHPA) whose methylated derivatives were divided by extraction with organic solvents is proposed. The method sensitivity for DA is 0.25 ng in a sample and for DHPA 0.1 ng in a sample. The yield of the internal standard is 80-85%. The method is high specific, the cross reaction during determination of DA and DHPA does not exceed 5%. The study of the effects of some dopaminergic agents (apomorphine, haloperidol. L-DOPA and pargyline on the contents of DA and DHPA in the rat brain basal ganglia in vivo confirms a high specificity of the method. The method may be used for screening of pharmacological compounds possessing the suggested dopaminergic and antimonoamine oxidase activity.
Petrenko
S V
SV
Markova
S V
SV
Gomolko
N N
NN
D015102:3,4-Dihydroxyphenylacetic Acid / Q000032:analysis*; D000818:Animals; D001479:Basal Ganglia / Q000032:analysis / Q000187:drug effects; D001923:Brain Chemistry / Q000187:drug effects; D002394:Catechol O-Methyltransferase; D004298:Dopamine / Q000032:analysis*; D015259:Dopamine Agents / Q000494:pharmacology; D008297:Male; D008722:Methods; D008745:Methylation; D010648:Phenylacetates / Q000032:analysis*; D051381:Rats; D014316:Tritium
D015259:Dopamine Agents; D010648:Phenylacetates; D014316:Tritium; D015102:3,4-Dihydroxyphenylacetic Acid; D002394:Catechol O-Methyltransferase; D004298:Dopamine
Bystryĭ radiofermentnyĭ metod opredeleniia dofamina i 3,4-dioksifeniluksusnoĭ kisloty v bazal'nykh gangliiakh mozga krys.