Simultaneous recording of presynaptic spikes and excitatory postsynaptic potentials from monosynaptically connected hippocampal neurons.
A technique has been devised to activate single granule cells in the hippocampus, and to record simultaneously spikes from the particular granule cell and excitatory postsynaptic potentials from a monosynaptically connected CA3 neuron. The unitary excitatory postsynaptic potentials (EPSPs) sustained for long observation periods, and increased in size with increases in stimulus frequency and in external Ca2+ concentration. This technique may be useful for quantal analysis of transmission through the synapse between mossy fibers and CA3 neurons.
Sawada
S
S
Department of Physiology, Faculty of Medicine, Kanazawa University, Japan.
Kamiya
H
H
Yamamoto
C
C
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D002122:Calcium Chloride / Q000494:pharmacology; D005071:Evoked Potentials / Q000187:drug effects; D005971:Glutamates / Q000494:pharmacology; D018698:Glutamic Acid; D006168:Guinea Pigs; D006624:Hippocampus / Q000166:cytology / Q000502:physiology*; D008839:Microelectrodes; D009474:Neurons / Q000502:physiology*; D013569:Synapses / Q000502:physiology*
D005971:Glutamates; D018698:Glutamic Acid; D002122:Calcium Chloride