title: Enhancement of 5'-nucleotidase activity of human leukemic cells after fractionation: implications for cancer and aging.
abstract: Previous studies reported that 5'-nucleotidase activity was undetectable or at much lower levels in the homogenate of human chronic lymphocytic leukemic (CCL) cells than in normal lymphocytes. In the present study, 5'-nucleotidase specific activity in acute myelocytic leukemia (AML), which varied in a range from undetectable to 1.4 (nmoles/min.mg protein), was enhanced by cell fractionation, from undetectable in the homogenate, up to 18.8 +/- 1.2, 6.4 +/- 0.7 and 0.68 +/- 0.12 in plasma membranes, microsomes, and cytosol fraction, respectively. In a further fractionation of the cytosol of various leukemic cells with ammonium sulfate, 5'-nucleotidase specific activity increased up to 14-fold in the 60% (NH4)2SO4 fraction, with a recovery of 1266 +/- 115%. These data suggest that 5'-nucleotidase activity in fractionated leukemic cells is higher than reported previously and that the sum of 5'-nucleotidase activity in subcellular compartments is higher than that detected in the homogenate. Furthermore, even when 5'-nucleotidase was undetectable in a homogenate, it became detectable in the plasma membranes, suggesting that its ecto-enzyme function is still active in leukemic cells. The undetectable or low 5'-nucleotidase in the homogenate is indicative of (1) the enzyme itself being in an inactive form but becoming active after the fractionations, or (2) the presence of a factor(s) that prevents the enzyme from being detected but that is separated from the enzyme by the fractionations. In both cases, the rate of nucleotide catabolism by inactive 5'-nucleotidase in rapidly proliferating leukemic cells should be slower than when the enzyme is active. The present finding is consistent with our previous findings that during normal cell aging the high 5'-nucleotidase activity is associated with senescent non-proliferating cells but low or undetectable activity with rapidly proliferating immortal cells. The implications of 5'-nucleotidase for DNA synthesis in aging and cancer are discussed.
authors.affiliation: Department of Neoplastic Diseases, Mount Sinai School of Medicine, New York, NY 10029.
authors.forename: A S
authors.initials: AS
authors.lastname: Sun
authors.forename: M
authors.initials: M
authors.lastname: Renaud
mesh_terms: D015720:5'-Nucleotidase / Q000097:blood* / Q000302:isolation & purification; D000293:Adolescent; D000328:Adult; D000368:Aged; D003600:Cytosol / Q000201:enzymology; D005260:Female; D006801:Humans; D007700:Kinetics; D007938:Leukemia / Q000097:blood / Q000201:enzymology*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000201:enzymology; D015470:Leukemia, Myeloid, Acute / Q000201:enzymology; D007962:Leukocytes / Q000201:enzymology*; D008214:Lymphocytes / Q000201:enzymology; D008297:Male; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000201:enzymology; D012016:Reference Values; D013347:Subcellular Fractions / Q000201:enzymology
chemical_list: D015720:5'-Nucleotidase