Long-term study of chimaerism in bone marrow transplantation recipients for severe aplastic anaemia.
We used minisatellite probes to analyse by DNA fingerprints the long-term engraftment (median 4.3 years, range 1-2) of 21 bone marrow transplantation recipients for severe aplastic anaemia. Patients received their graft from histocompatible siblings. They were conditioned with cyclophosphamide (150 mg/kg) and a 6GY thoracoabdominal irradiation and did not have ex-vivo T cell depletion of marrow donor. DNA was extracted peripheral mononuclear cells and analysed by Southern blotting with 32P-labelled single-stranded RNA probes. Seven out of 21 donor-recipient pairs were sex-mismatched and additionally studied with a probe detecting a male specific repeated sequence on the Y chromosome. Red cell surface phenotype was also used as marker of engraftment in most cases. Long-term engraftment appeared complete for all patients studied with respect to the three methods.
Keable
H
H
Laboratoire d'Oncologie Moléculaire, UA 1158 CNRS, Institut Gustave Roussy, Villejuif, France.
Bourhis
J H
JH
Brison
O
O
Lehn
P
P
Schenmetzler
C
C
Devergie
A
A
Gluckman
E
E
D000293:Adolescent; D000328:Adult; D000741:Anemia, Aplastic / Q000235:genetics* / Q000628:therapy; D000954:Antigens, Surface / Q000032:analysis; D016026:Bone Marrow Transplantation; D002648:Child; D002675:Child, Preschool; D002678:Chimera; D004912:Erythrocytes / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D013997:Time Factors
D000954:Antigens, Surface