Characterisation of human thyroid epithelial cells immortalised in vitro by simian virus 40 DNA transfection.
Human primary thyroid follicular epithelial cells were transfected with a plasmid containing an origin-defective SV40 genome (SVori-) to produce several immortal cell lines. Two of the 10 cell lines analysed expressed specific features of thyroid epithelial function (iodide-trapping and thyroglobulin production). These two lines were characterised in detail and found to be growth factor-independent, capable of anchorage-independent growth at low frequency but non-tumorigenic in nude mice. These differentiated, These differentiated, partially transformed cell lines were shown to be suitable for gene transfer at high frequency using simple coprecipitation techniques.
Lemoine
N R
NR
ICRF Molecular Oncology Group, Hammersmith Hospital, London, UK.
Mayall
E S
ES
Jones
T
T
Sheer
D
D
McDermid
S
S
Kendall-Taylor
P
P
Wynford-Thomas
D
D
D000328:Adult; D000818:Animals; D002460:Cell Line; D004279:DNA, Viral; D004848:Epithelium / Q000648:ultrastructure; D005260:Female; D005819:Genetic Markers; D006801:Humans; D007621:Karyotyping; D051379:Mice; D008819:Mice, Nude; D013539:Simian virus 40 / Q000235:genetics*; D012974:Sodium Iodide / Q000378:metabolism; D013954:Thyroglobulin / Q000032:analysis; D013961:Thyroid Gland / Q000378:metabolism / Q000648:ultrastructure; D014162:Transfection
D004279:DNA, Viral; D005819:Genetic Markers; D013954:Thyroglobulin; D012974:Sodium Iodide