A simplified method for the characterization of nuclear polyhedrosis virus genomes.
A simplified restriction endonuclease analysis procedure is described which allows the characterization of baculovirus DNA obtained directly from a single larvae without purification of virus. This rapid method was used to demonstrate the genomic stability of nuclear polyhedrosis viruses (NPVs) from Agrotis segetum, Euxoa messoria and Mamestra brassicae after several passages in Euxoa scandens.
Hamelin
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C
Centre de Recherche en Virologie, Institut Armand-Frappier, Québec, Canada.
Lavallée
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C
Belloncik
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S
D000818:Animals; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis; D005814:Genes, Viral; D005821:Genetic Techniques; D007304:Insect Viruses / Q000235:genetics*; D007313:Insecta / Q000382:microbiology; D007814:Larva / Q000382:microbiology; D012692:Serial Passage; D013045:Species Specificity
D004279:DNA, Viral; D004262:DNA Restriction Enzymes