Expression of adhesion structures during B cell development in man.
We have used three-color flow cytometry to study the expression of adhesion structures during B cell development in man. The results indicate that the cell-surface molecule(s) recognized by 515, a mAb which defines a broadly expressed family of cell-surface glycoproteins that includes lymphocyte homing receptors, exhibit a clear bimodal distribution (515lo and 515hi); 515hi cells were found exclusively on more mature B cells which already expressed high levels of CD20. Earlier, less mature B cells, identified by their expression of CD10, were uniformly 515lo. In contrast, the CD11a/LFA-1 Ag was acquired gradually over the course of B cell development. B cells which expressed high levels of CD20 expressed three to six times as much CD11a/LFA-1 as cells which expressed CD10. Interestingly, expression of the 515hi phenotype was tightly correlated with that of Leu-8, a marker previously shown to define maturational and functions subsets of B cells. These data document the coordinated regulation of multiple cell surface structures during B cell ontogeny, and demonstrate that adhesion structures necessary for proper B cell function are precisely up-regulated during B cell differentiation in man.
Kansas
G S
GS
Department of Pathology, University of Iowa College of Medicine, Iowa City 52242.
Dailey
M O
MO
D000328:Adult; D000943:Antigens, Differentiation / Q000032:analysis; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D001402:B-Lymphocytes / Q000032:analysis / Q000145:classification / Q000502:physiology*; D018845:CD11 Antigens; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules; D002454:Cell Differentiation; D006801:Humans; D016169:Lymphocyte Function-Associated Antigen-1; D010641:Phenotype
D000943:Antigens, Differentiation; D000944:Antigens, Differentiation, B-Lymphocyte; D000954:Antigens, Surface; D018845:CD11 Antigens; D015815:Cell Adhesion Molecules; D016169:Lymphocyte Function-Associated Antigen-1