title: Confirmation and refinement of the localisation of the c-MEL locus on chromosome 19 by physical and genetic mapping.
abstract: The human gene locus c-MEL was identified following transfection of genomic DNA from the human melanoma cell line NK14; it has previously been assigned to chromosome 19 (p13.2-q13.2) by analysis of somatic cell hybrids. We have further refined the position of this gene to the proximal region of 19p (cen-p13.2), using cell hybrids containing only fragments of human chromosome 19. We have confirmed this physical localisation by linkage analysis with a recently described restriction fragment length polymorphism for the c-MEL gene, and mapped the locus within the region of the low density lipoprotein receptor gene (LDLR) (Lod 4.43, theta = 0.10) and the anonymous marker D19S11 (13.1.25) (Lod 9.33, theta = 0). This gene thus maps to a region of chromosome 19 involved in karyotypic abnormalities in a variety of malignancies including melanomas and leukaemias.
authors.affiliation: Department of Biochemistry and Molecular Genetics, St. Mary's Hospital Medical School, Paddington, London, UK.
authors.forename: E
authors.initials: E
authors.lastname: Nimmo
authors.forename: R A
authors.initials: RA
authors.lastname: Padua
authors.forename: D
authors.initials: D
authors.lastname: Hughes
authors.forename: J D
authors.initials: JD
authors.lastname: Brook
authors.forename: R
authors.initials: R
authors.lastname: Williamson
authors.forename: K J
authors.initials: KJ
authors.lastname: Johnson
mesh_terms: D002460:Cell Line; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19; D015342:DNA Probes; D005796:Genes; D008040:Genetic Linkage; D006801:Humans; D006822:Hybrid Cells; D012150:Polymorphism, Restriction Fragment Length; D014162:Transfection
chemical_list: D015342:DNA Probes