Estrone sulfate and sulfatase activity in human breast cancer and endometrial cancer.
Estrone sulfate (E1-S) in the serum and tissues of patients with breast cancer or endometrial cancer was measured by a direct radioimmunoassay without hydrolysis. The concentration of E1-S in breast cancer tissue was 1.64 +/- 0.28 ng/g wet wt (+/- SE), lower than in surrounding normal breast tissue (4.46 +/- 1.23). Estradiol-17 beta(E2)/E1-S was higher in endometrial cancer tissue than normal endometrial tissue. Estrone sulfatase activity in breast cancer tissue was 0.81 +/- 0.23 nmol/h/mg protein, higher than in surrounding normal breast tissue (0.35 +/- 0.11). These results suggest that E1-S, which is abundant in the peripheral circulation, is hydrolyzed by sulfatase in breast cancer tissue or endometrial cancer tissue and liberates free estrogens, which may stimulate the growth of these malignant tumors.
Naitoh
K
K
Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Japan.
Honjo
H
H
Yamamoto
T
T
Urabe
M
M
Ogino
Y
Y
Yasumura
T
T
Nambara
T
T
D000273:Adipose Tissue / Q000201:enzymology; D001943:Breast Neoplasms / Q000201:enzymology / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism; D004966:Estrogens, Conjugated (USP) / Q000378:metabolism*; D004970:Estrone / Q000031:analogs & derivatives* / Q000378:metabolism; D005260:Female; D006801:Humans; D008875:Middle Aged; D011863:Radioimmunoassay; D013429:Sulfatases / Q000378:metabolism*; D014594:Uterine Neoplasms / Q000201:enzymology / Q000378:metabolism*
D004966:Estrogens, Conjugated (USP); D004970:Estrone; D004958:Estradiol; D013429:Sulfatases; C020121:estrone sulfatase; C017296:estrone sulfate