Redistributive properties of the vesicular stomatitis virus polymerase.
The template for transcription of the vesicular stomatitis virus (VSV) genome consists of a negative-strand RNA (approximately 11 kb) tightly associated with approximately 1250 copies of the nucleocapsid or N protein (N-RNA template). The interaction between the virion-associated polymerase and this template was probed with a novel assay using purified N-RNA complexes added to detergent-disrupted uv-irradiated standard virions or unirradiated defective interfering (DI) particles. In contrast to the well-known stability of assembled cellular transcription complexes, the VSV polymerase copied exogenously added templates efficiently and yielded products indistinguishable from control virus transcription. Addition of uv-irradiated N-RNA templates to unirradiated virus effectively competed for transcription of endogenous template indicating that most or all of the polymerase can freely redistribute. Furthermore preincubation of virus and added templates at high ionic strength to solubilize L and NS polymerase proteins did not release additional active enzyme for redistribution. Pretranscription of virus also had little or no effect on redistributed activity indicating that polymerase complexes are capable of multiple rounds of synthesis beginning at the 3' end promoter. Unexpectedly, titration with saturating amounts of added N-RNA showed that active polymerase complexes are only in slight excess relative to template in standard or DI particles despite the large surplus of packaged L and NS polypeptides. Moreover, added standard virus templates competed equally well for the redistributing polymerase from DI particles or standard virus indicating no significant polymerase-binding preference for interfering templates. These findings bear important implications regarding mechanisms of VSV transcription and replication.
Helfman
W B
WB
Department of Biology and Molecular Biology Institute, San Diego State University, California 92182.
Perrault
J
J
D000255:Adenosine Triphosphate / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D003673:Defective Viruses / Q000235:genetics; D046911:Macromolecular Substances; D008970:Molecular Weight; D009994:Osmolar Concentration; D011485:Protein Binding; D012367:RNA, Viral / Q000235:genetics; D013698:Templates, Genetic; D014158:Transcription, Genetic / Q000528:radiation effects; D014466:Ultraviolet Rays; D014721:Vesicular stomatitis Indiana virus / Q000201:enzymology* / Q000235:genetics
D046911:Macromolecular Substances; D012367:RNA, Viral; D000255:Adenosine Triphosphate; D012321:DNA-Directed RNA Polymerases