[Production, purification and evaluation of human cytomegalovirus 66 kD structural antigen for antibody detection by ELISA].
An ELISA method using human CMV major polypeptide (66 kD) purified by electroelution was used to detect IgG and IgM antibodies and was compared with a conventional ELISA using the whole virus. The ELISA-66 kD showed a good sensitivity (98.2%) and had a better specificity than the ELISA-whole virus (97.5% versus 80.3%). Cross reactions observed, particularly with sera containing anti-EBV antibodies, were ruled out.
Gireaudot
P
P
Service de Bactériologie Virologie B, Centre Hospitalier Regional, IRFPPS, Lille, France.
Dewilde
A
A
Lobert
P E
PE
Bocket-Mouton
L
L
Wattre
P
P
D000956:Antigens, Viral / Q000276:immunology / Q000302:isolation & purification*; D003587:Cytomegalovirus / Q000276:immunology* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis*
D000956:Antigens, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M
Production, purification et évaluation de l'antigène de structure 66 kD du cytomégalovirus humain pour la détection des anticorps par ELISA.