Restriction endonuclease analysis of infectious laryngotracheitis viruses: comparison of modified-live vaccine viruses and North Carolina field isolates.
Six modified-live (ML) infectious laryngotracheitis (ILT) vaccine viruses, three reference strains, and 18 field isolates were compared by restriction endonuclease analysis of their DNA. Viral DNA digestion patterns were established for vaccine viruses using restriction endonucleases PstI, BamHI, KpnI, and HindIII. Using these enzymes, five of six ML vaccine viruses had identical restriction endonuclease cleavage patterns. Vaccine viruses had distinct patterns compared with ILT virus reference strains Illinois-N71851, Cover, and NVSL. Restriction endonuclease cleavage patterns of 18 field isolates of ILT virus, obtained from ILT outbreaks in North Carolina, were indistinguishable from vaccine viruses. These results suggest a possible role of vaccine or vaccine-like viruses in recent ILT outbreaks.
Guy
J S
JS
Department of Microbiology, College of Veterinary Medicine, North Carolina State University, Raleigh 27606.
Barnes
H J
HJ
Munger
L L
LL
Rose
L
L
D000818:Animals; D002645:Chickens / Q000382:microbiology*; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis*; D006564:Herpesviridae / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D006566:Herpesviridae Infections / Q000382:microbiology / Q000662:veterinary*; D009657:North Carolina; D011201:Poultry Diseases / Q000382:microbiology*; D015183:Restriction Mapping; D014765:Viral Vaccines
D004279:DNA, Viral; D014765:Viral Vaccines; D004262:DNA Restriction Enzymes