Effect of ammonium chloride on the astrocyte benzodiazepine receptor.
We have carried out studies on the effect of ammonium chloride on the astrocyte benzodiazepine receptor. Scatchard analysis of the binding of [3H]Ro-5-4864 to homogenates prepared from primary astrocyte cultures showed a significant decrease in Kd (27% with 2 mM NH4Cl; 32% with 5 mM NH4Cl; 25% with 10 mM NH4Cl) and Bmax (14% with 10 mM NH4Cl). These findings indicate that ammonium chloride can affect the astrocyte benzodiazepine receptor, and that such receptor changes may contribute to ammonia-induced encephalopathy.
Ducis
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Department of Pathology, University of Miami/Jackson Memorial Hospital, FL.
Norenberg
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Norenberg
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D000643:Ammonium Chloride / Q000494:pharmacology*; D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D001570:Benzodiazepinones / Q000378:metabolism; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D003292:Convulsants / Q000494:pharmacology; D007700:Kinetics; D051381:Rats; D011916:Rats, Inbred F344; D011963:Receptors, GABA-A / Q000187:drug effects / Q000378:metabolism*
D001570:Benzodiazepinones; D003292:Convulsants; D011963:Receptors, GABA-A; D000643:Ammonium Chloride; C028513:4'-chlorodiazepam