L-type calcium channels in the regulation of neurite outgrowth from rat dorsal root ganglion neurons in culture.
Dorsal root ganglion neurons from neonatal rats were grown in culture for 12 h and the extent of neurite outgrowth determined by counting the fraction of neurons with neurites. In the presence of high K+, veratridine or bradykinin the extent of neurite outgrowth was reduced by about 60%. The inhibitory effect of depolarisation was reversible and was abolished by nifedipine. gamma-Aminobutyric acid (GABA), baclofen and 2-chloroadenosine had no effect on neurite outgrowth in control cultures but abolished the inhibitory effect of depolarisation.
Robson
S J
SJ
Physiological Laboratory, University of Liverpool, U.K.
Burgoyne
R D
RD
D015762:2-Chloroadenosine / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D001369:Axons / Q000187:drug effects / Q000502:physiology*; D001418:Baclofen / Q000494:pharmacology; D001920:Bradykinin / Q000494:pharmacology; D015220:Calcium Channels / Q000502:physiology*; D002478:Cells, Cultured; D005727:Ganglia, Spinal / Q000648:ultrastructure*; D009474:Neurons / Q000648:ultrastructure*; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D014701:Veratridine / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D015220:Calcium Channels; D015762:2-Chloroadenosine; D005680:gamma-Aminobutyric Acid; D014701:Veratridine; D001418:Baclofen; D011188:Potassium; D001920:Bradykinin