title: Molecular cloning and sequence analysis of cDNA encoding rat adrenal cytochrome P-450(11)beta.
abstract: A cDNA clone encoding cytochrome P-450(11)beta of rat adrenal has been cloned and sequenced using a bovine P-450(11)beta cDNA insert (pcP-450(11 beta)-2; (1987) J. Biochem. 102, 559-568) as a probe. The nucleotide sequence contains an open reading frame sufficient to encode the entire amino acid sequence of a P-450(11)beta precursor protein consisting of 499 amino acids including an extension peptide of 24 amino acids at the NH2-terminus. The cDNA contains 1247 nucleotides at the 3'-noncoding region including 51 nucleotides of poly A, but lacks the 5'-noncoding region. The deduced amino acid sequence shows 61% similarity to that of bovine P-450(11)beta. Putative binding sites for heme and steroid are highly conserved among steroidogenic P-450s of known structure.
authors.affiliation: Department of Biochemistry, Osaka University Medical School, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Nonaka
authors.forename: N
authors.initials: N
authors.lastname: Matsukawa
authors.lastname: Morohashi
authors.forename: T
authors.initials: T
authors.lastname: Omura
authors.forename: T
authors.initials: T
authors.lastname: Ogihara
authors.forename: H
authors.initials: H
authors.lastname: Teraoka
authors.forename: M
authors.initials: M
authors.lastname: Okamoto
mesh_terms: D000311:Adrenal Glands / Q000201:enzymology*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D004247:DNA / Q000096:biosynthesis*; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity
chemical_list: D004251:DNA Transposable Elements; D004247:DNA; D003577:Cytochrome P-450 Enzyme System; D004262:DNA Restriction Enzymes