Identification and characterization of endothelin binding sites in rat renal papillary and glomerular membranes.
The present study was designed to identify and characterize specific endothelin binding sites in membranes of rat renal papillae and glomeruli which appear to be target tissues for this new peptide hormone. Saturation binding studies indicate that the sites have a high and uniform affinity. The dissociation constants averaged 662 +/- 151 and 1309 +/- 123 pM and the receptor densities 7666 +/- 920 and 5831 +/- 348 fmol/mg protein for papillary and glomerular membranes, respectively. Endothelin 1, endothelin 3 and sarafotoxin all inhibited [125I]-endothelin binding with IC50's in the 100-300 pM range, whereas unrelated peptides, namely angiotensin II, atrial natriuretic peptide, and platelet-derived growth factor failed to compete for [125I]-endothelin binding. Deletion of the carboxyterminal tryptophan in endothelin 1 reduced its affinity for glomerular binding sites by 2 orders of magnitude. Specific endothelin binding to these membranes was maximal at pH 4 and was markedly inhibited as the pH was raised above 8. When [125I]-endothelin is covalently linked to glomerular membrane binding sites, SDS-PAGE of these solubilized membranes followed by autoradiography reveals a predominant specifically labeled band of 45 kDa. Whether this band represents a subunit of the endothelin receptor(s), the receptor proper, or an intracellular endothelin binding protein remains to be determined.
Martin
E R
ER
Renal Division, Brigham and Women's Hospital, Boston, Massachusetts.
Marsden
P A
PA
Brenner
B M
BM
Ballermann
B J
BJ
D000818:Animals; D001667:Binding, Competitive; D002118:Calcium; D002462:Cell Membrane / Q000378:metabolism; D016232:Endothelins; D004730:Endothelium, Vascular / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007678:Kidney Glomerulus / Q000378:metabolism*; D007679:Kidney Medulla / Q000378:metabolism*; D007700:Kinetics; D008274:Magnesium; D008297:Male; D008345:Manganese; D008970:Molecular Weight; D010455:Peptides / Q000378:metabolism*; D051381:Rats; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000145:classification / Q000302:isolation & purification
D016232:Endothelins; D010455:Peptides; D011956:Receptors, Cell Surface; D008345:Manganese; D008274:Magnesium; D002118:Calcium