title: Genetic and molecular analyses of the gene encoding staphylococcal enterotoxin D.
abstract: The gene (entD) encoding staphylococcal enterotoxin D (SED) has been located on a 27.6-kilobase penicillinase plasmid designated pIB485. This plasmid was present in all SED-producing strains tested. The entD gene was cloned on a 2.0-kilobase DNA fragment and was expressed in Escherichia coli. Sequence analysis of this fragment revealed an open reading frame that encoded a 258-amino-acid protein that possessed a 30-amino-acid signal peptide. The 228-amino-acid mature polypeptide had a molecular weight of 26,360 and contained a high degree of sequence similarity to the other staphylococcal enterotoxins. S1 nuclease mapping showed that transcription of entD was initiated 266 nucleotides upstream from the translation start codon. The entD gene was also shown to be activated by the staphylococcal regulatory element known as agr.
authors.affiliation: Division of Biology, Kansas State University, Manhattan 66506.
authors.forename: K W
authors.initials: KW
authors.lastname: Bayles
authors.forename: J J
authors.initials: JJ
authors.lastname: Iandolo
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004720:Endonucleases; D004768:Enterotoxins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D005798:Genes, Bacterial; D005838:Genotype; D008969:Molecular Sequence Data; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013211:Staphylococcus aureus / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D004768:Enterotoxins; C019368:enterotoxin D, Staphylococcal; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases