Cholecystokinin elevates cytosolic calcium in small cell lung cancer cells.
The ability of cholecystokinin (CCK) to elevate intracellular Ca2+ levels in small cell lung cancer cells was investigated using the fluorescent Ca2+ indicator Fura 2. CCK-8 elevated the cytosolic Ca2+ levels in cell line NCI-H345 in a dose dependent manner. Nanomolar concentration of CCK-8 elevated cytosolic Ca2+ levels in the absence or presence of extracellular Ca2+. Potent CCK agonists such as gastrin-1 and nonsulfated CCK-8 but not inactive compounds such as CCK-27-32-NH2 elevated cytosolic Ca2+ levels. These data suggest that CCK receptors may regulate the release of Ca2+ from intracellular organelles in small cell lung cancer cells.
Staley
J
J
Department of Biochemistry and Molecular Biology, George Washington University School of Medicine and Health Sciences, Washington, D.C. 20037.
Fiskum
G
G
Moody
T W
TW
D001839:Bombesin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D018288:Carcinoma, Small Cell / Q000378:metabolism*; D002766:Cholecystokinin / Q000494:pharmacology*; D003600:Cytosol / Q000378:metabolism; D008175:Lung Neoplasms / Q000378:metabolism*; D009496:Neurotensin / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000502:physiology; D012844:Sincalide / Q000494:pharmacology; D013329:Structure-Activity Relationship
D011956:Receptors, Cell Surface; D009496:Neurotensin; D002766:Cholecystokinin; D012844:Sincalide; D001839:Bombesin; D002118:Calcium