[Identification of human porins. I. Purification of a porin from human B-lymphocytes (Porin 31HL) and the topochemical proof of its expression on the plasmalemma of the progenitor cell].
We describe for the first time a porin (Porin 31HL) on the plasmalemm of an eukaryontic cell line, where porins have been found only on the outer mitochondrial membranes. The expression of the porin on the plasmalemm of transformed human B-lymphocytes is demonstrated by cytotoxicity- and indirect immunofluorescence techniques with living and fixed cells. The rabbit xenoantisera used were directed against purified Porin 31HL and free or acetylated synthetic peptides of its nineteen N-terminal amino acids. The three-step purification procedure for Porin 31HL started from a total membrane fraction of the B-cell line, followed by ion-exchange chromatography on CM- and DEAE-cellulose and a final gel filtration in SDS on Sephacryl S-300.
Thinnes
F P
FP
Max-Planck-Institut für experimentelle Medizin, Abteilung Immunchemie, Göttingen.
Götz
H
H
Kayser
H
H
Benz
R
R
Schmidt
W E
WE
Kratzin
H D
HD
Hilschmann
N
N
D000596:Amino Acids; D000818:Animals; D001402:B-Lymphocytes / Q000032:analysis*; D001425:Bacterial Outer Membrane Proteins / Q000302:isolation & purification*; D002460:Cell Line; D002462:Cell Membrane / Q000032:analysis*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005455:Fluorescent Antibody Technique; D006801:Humans; D008928:Mitochondria / Q000032:analysis; D008969:Molecular Sequence Data; D018272:Porins; D011817:Rabbits
D000596:Amino Acids; D001425:Bacterial Outer Membrane Proteins; D018272:Porins
Zur Kenntnis der Porine des Menschen. I. Reinigung eines Porins aus menschlichen B-Lymphozyten (Porin 31HL) und sein topochemischer Nachweis auf dem Plasmalemm der Herkunftszelle.