Structure of the alpha 1 subunit of horse Na,K-ATPase gene.
Genomic DNA for Na,K-ATPase alpha 1 subunit was obtained from libraries of horse kidney genomic DNA in Charon 4A and in EMBL3 bacteriophages by screening with the full sized cDNA probe of the alpha 1 subunit of rat Na,K-ATPase as probe. The gene spans 30 kb and consists of 23 exons and 22 intervening sequences. Intron-exon boundaries were analyzed. The protein-coding nucleotide sequence encodes 1016 amino acids with an Mr of 112,264. The putative amino acid sequence of horse alpha 1 is 96-97% homologous to those of other mammalian species.
Kano
I
I
Department of Toxicology, Tokyo Metropolitan Research Laboratory of Public Health, Japan.
Nagai
F
F
Satoh
K
K
Ushiyama
K
K
Nakao
T
T
Kano
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005091:Exons; D005796:Genes; D006736:Horses; D006801:Humans; D007679:Kidney Medulla / Q000201:enzymology; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics*; D013045:Species Specificity
D046911:Macromolecular Substances; D000254:Sodium-Potassium-Exchanging ATPase