title: The glycosphingolipid composition of the human hepatoma cell line,Hep-G2.
abstract: The origin of plasma glycosphingolipids in normal individuals and the mechanisms by which tumor-associated glycosphingolipid antigens enter the plasma in patients with cancer are largely unknown. The Hep-G2 human hepatoma cell line retains many of the characteristics of differentiated hepatocytes including the ability to synthesize and secrete lipoproteins. Preliminary results indicated that newly synthesized Hep-G2 cell glycosphingolipids are coupled to the secreted lipoproteins. This suggests that this cell line may offer an interesting model for studying glycosphingolipid secretion, transfer, and shedding. We now report on the chemical and immunological characterization of Hep-G2 cell glycosphingolipids. Five major glycosphingolipids were purified and biochemically characterized: glycosylceramide, lactosyl ceramide, ceramide trihexoside, ganglioside GM3, and lactosyl sulfatide. Four additional minor components (3-fucosyl-lactosamine containing glycolipids, asialo GM2, galactosylgloboside, and ganglioside GM1) were identified using a combination of exoglycosidase digestion and immunostaining of thin-layer chromatography plates with specific carbohydrate binding proteins. This demonstrates that although this cell line synthesizes a limited number of major glycosphingolipids, it retains the ability to produce at least small amounts of structures in the lactoneo, globo, and ganglio series of glycosphingolipids. These studies show that it will be possible to investigate the mechanisms of secretion by Hep-G2 cells of different classes of these molecules such as neutral glycosphingolipids, gangliosides, and sulfatides.
authors.affiliation: Department of Pathology and Laboratory Medicine, Unversity of Pennsylvania, Philadelphia 19104.
authors.forename: P F
authors.initials: PF
authors.lastname: Spitalnik
authors.forename: J M
authors.initials: JM
authors.lastname: Danley
authors.forename: S R
authors.initials: SR
authors.lastname: Burger
authors.forename: S L
authors.initials: SL
authors.lastname: Spitalnik
mesh_terms: D000911:Antibodies, Monoclonal; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D006017:Glycolipids / Q000378:metabolism; D006028:Glycosphingolipids / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D008113:Liver Neoplasms; D011863:Radioimmunoassay; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D006017:Glycolipids; D006028:Glycosphingolipids