Flow cytometric analysis of human lymphocytes using affinity-purified antibody to T cell receptor beta synthetic J region peptide.
The purpose of this study was to use affinity-purified polyclonal antibodies produced against a synthetic peptide corresponding to the joining (J) region of a human T cell receptor beta chain to characterize antigen receptor expression on subpopulations of human lymphocytes. The synthetic peptide used was ANYGYTFGSGTRLTVV, corresponding to the J segment of the human beta-chain gene YT35. Biochemical characterization has previously demonstrated binding of anti-J beta peptide antibodies to the alpha/beta heterodimer and to certain immunoglobulin light chains. Flow cytometric analysis of normal human peripheral blood lymphocytes performed here, using affinity-purified antibodies to the J beta peptide, showed expression of the epitope on 50-60% of CD20 (B1)-positive B lymphocytes, and on 40-50% of CD8-positive T lymphocytes. Only background levels were observed on CD4-positive T cells.
Shankey
T V
TV
Department of Urology, Loyola Stritch School of Medicine, Maywood, Illinois 60153.
Schluter
S F
SF
Marchalonis
J J
JJ
D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D018801:CD2 Antigens; D005434:Flow Cytometry; D006801:Humans; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D016693:Receptors, Antigen, T-Cell, alpha-beta; D011971:Receptors, Immunologic / Q000032:analysis
D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D018801:CD2 Antigens; D010455:Peptides; D011948:Receptors, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta; D011971:Receptors, Immunologic