A negative regulatory element in the promoter of the human alpha 1-antitrypsin gene.
A "minimal tissue-specific element" of the human alpha 1-antitrypsin (alpha 1-AT) gene promoter, located in the -137/-37 region, is able to drive hepatoma-specific transcription from the heterologous SV40 promoter. Here we show that, in HeLa cells, this element is inactivated by a negative regulatory mechanism. This negative control can be overcome by deletion or mutation of two specific DNA domains.
De Simone
V
V
European Molecular Biology Laboratory (EMBL), Heidelberg, FRG.
Cortese
R
R
D001483:Base Sequence; D006528:Carcinoma, Hepatocellular; D002460:Cell Line; D005796:Genes; D005809:Genes, Regulator; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D008113:Liver Neoplasms; D008969:Molecular Sequence Data; D009708:Nucleotidases; D009710:Nucleotide Mapping; D010957:Plasmids; D011401:Promoter Regions, Genetic; D013539:Simian virus 40 / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection; D000515:alpha 1-Antitrypsin / Q000235:genetics*
D000515:alpha 1-Antitrypsin; D009708:Nucleotidases