title: Construction of cloning/sequencing vectors with an alternative polylinker.
abstract: This paper describes the construction of a new plasmid and M13 phage cloning vector in which the 54-bp polylinkers of pUC19 and of M13mp8 are replaced with a 45-bp chemically synthesized polylinker with different restriction sites. The new polylinker is inserted in frame at the N-terminal end of the beta galactosidase lac Z fragment. The plasmid was named pLH1, the phage M13LH1.
authors.affiliation: Gene Expression Section, Amersham International plc, Bucks, United Kingdom.
authors.forename: L A
authors.initials: LA
authors.lastname: Howard
authors.forename: S A
authors.initials: SA
authors.lastname: Ortlepp
mesh_terms: D001435:Bacteriophages / Q000235:genetics; D001483:Base Sequence; D001709:Biotechnology; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010957:Plasmids
chemical_list: D004247:DNA; D004262:DNA Restriction Enzymes