Pathogenesis of Hantaan virus in mice.
The virulence of two virus clones (HV cl-1 and HV cl-2) of Hantaan virus, which were plaque-purified on Vero E6 cells, were compared in suckling mice infected by the intraperitoneal or intracerebral route. HV cl-1 increased the mortality of the mice whereas HV cl-2 did not. Furthermore, virus of high titre was isolated from various organs of mice infected with HV cl-1 and high titres were maintained, whereas after infection with HV cl-2, virus was isolated from various organs only in low titre and only temporarily. HV cl-1 strongly induced cell-to-cell fusion, but the cell fusion activity was at a minimum level in cells infected with HV cl-2. However these two clones induced similar titres of antibodies in mice. Cytotoxic T lymphocyte assays against macrophages infected with homologous and heterologous virus showed that cytotoxic T cell activity was induced in mice infected with HV cl-2, but suppressed in mice infected with HV cl-1. These results suggest that an alteration in the cell fusion function and the cytotoxic T cell activity are important in the pathogenesis of Hantaan virus infection in newborn mice.
Tamura
M
M
Department of Virology, Osaka University, Japan.
Asada
H
H
Kondo
K
K
Tanishita
O
O
Kurata
T
T
Yamanishi
K
K
D000818:Animals; D000831:Animals, Newborn; D000914:Antibodies, Viral / Q000096:biosynthesis; D000956:Antigens, Viral / Q000032:analysis; D002459:Cell Fusion; D003602:Cytotoxicity, Immunologic; D006476:Orthohantavirus / Q000254:growth & development / Q000276:immunology / Q000472:pathogenicity*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000276:immunology / Q000382:microbiology*; D051379:Mice; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014709:Vero Cells
D000914:Antibodies, Viral; D000956:Antigens, Viral