title: Detection of HIV-1 DNA in different subsets of human peripheral blood mononuclear cells using the polymerase chain reaction. Rapid communication.
abstract: The presence of HIV-1 proviral DNA was determined in CD4 cells, CD8 cells and macrophages/monocytes obtained from peripheral blood of 8 HIV-1 infected persons. Using the polymerase chain reaction (PCR) we were able to detect proviral DNA in the extracts of only 10(2)-10(3) CD4 (T4) cells. In contrast, 10(5) CD8 cells did not contain detectable amounts of proviral DNA. Surprisingly, in four of our eight patients studied no HIV-1 DNA was found in macrophages. In peripheral blood, every hundred T4 cell may be infected with HIV-1.
authors.affiliation: Virological Department, Bernhard-Nocht Institute of Tropical Medicine, Hamburg, Federal Republic of Germany.
authors.forename: F T
authors.initials: FT
authors.lastname: Hufert
authors.forename: D
authors.initials: D
authors.lastname: von Laer
authors.forename: C
authors.initials: C
authors.lastname: Schramm
authors.forename: A
authors.initials: A
authors.lastname: Tárnok
authors.forename: H
authors.initials: H
authors.lastname: Schmitz
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology*; D015496:CD4-Positive T-Lymphocytes / Q000032:analysis / Q000382:microbiology; D004279:DNA, Viral / Q000032:analysis*; D004259:DNA-Directed DNA Polymerase; D005784:Gene Amplification; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000032:analysis* / Q000382:microbiology; D011533:Proviruses / Q000235:genetics / Q000302:isolation & purification; D050378:T-Lymphocytes, Regulatory / Q000032:analysis / Q000382:microbiology; D019914:Taq Polymerase
chemical_list: D004279:DNA, Viral; D019914:Taq Polymerase; D004259:DNA-Directed DNA Polymerase