title: Involvement of a type 1 protein phosphatase encoded by bws1+ in fission yeast mitotic control.
abstract: Fission yeast cdc25+ and wee1+ interact genetically with cdc2+ in the regulation of cell division, respectively as a mitotic activator and inhibitor. cdc25+ is normally essential for mitosis, but this requirement is alleviated in a loss-of-function wee1 mutant background. A plasmid-borne sequence, other than wee1+, that causes a cdc25ts wee1- double mutant to revert to a temperature-sensitive cdc phenotype has been isolated. The gene carried by this plasmid is called bws1+ (for bypass of wee suppression). bws1+ also bypasses the ability of alleles of cdc2 that confer a wee phenotype (cdc2w) to suppress loss-of-function cdc25 mutants. The nucleotide sequence of bws1+ shows that the predicted protein shares 81% amino acid identity with the catalytic subunit of mammalian type 1 protein phosphatase. Thus a genetic screen that might have yielded a protein kinase (wee1+) uncovered a phosphatase that also appears to be involved in the pathway of mitotic control.
authors.affiliation: Cold Spring Harbor Laboratory, New York 11724.
authors.forename: R
authors.initials: R
authors.lastname: Booher
authors.forename: D
authors.initials: D
authors.lastname: Beach
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004720:Endonucleases / Q000494:pharmacology; D005656:Fungal Proteins / Q000235:genetics; D005800:Genes, Fungal; D005804:Genes, Lethal; D008938:Mitosis; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D010749:Phosphoprotein Phosphatases / Q000235:genetics*; D004718:Saccharomycetales / Q000235:genetics*; D012568:Schizosaccharomyces / Q000235:genetics*; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014158:Transcription, Genetic
chemical_list: D005656:Fungal Proteins; D004720:Endonucleases; D010749:Phosphoprotein Phosphatases; D015719:Single-Strand Specific DNA and RNA Endonucleases