Two genes encode 7SL RNAs in the yeast Yarrowia lipolytica.
We have identified an abundant cytoplasmic 7S RNA in crude extracts of the yeast Yarrowia lipolytica. A cDNA probe was prepared from this RNA and used to screen a genomic library. The DNA sequence of a positive clone was determined and the end positions of the 7S RNA gene established by comparison with the sequence of the extremities of 7S RNA. This gene, designated SCR2, encodes a 270-nucleotide RNA that can be folded into a secondary structure similar to that of 7SL RNAs. This RNA is 94.4% homologous to a previously identified 7S RNA from this yeast, but is encoded by a separate gene with highly divergent flanking sequences.
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Laboratoire de génétique des microorganismes, Institut National A gronomique, Thiverval Grignon, France.
Beckerich
J M
JM
Ribes
V
V
Tollervey
D
D
Gaillardin
C M
CM
D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012331:RNA, Fungal / Q000235:genetics*; D015183:Restriction Mapping; D012261:Ribonucleoproteins / Q000235:genetics*; D004718:Saccharomycetales / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D018271:Signal Recognition Particle
D004271:DNA, Fungal; D012331:RNA, Fungal; D012261:Ribonucleoproteins; D018271:Signal Recognition Particle; D004247:DNA