title: The human chromogranin A gene: chromosome assignment and RFLP analysis.
abstract: Chromogranin A/secretory protein I (CgA) is a glycoprotein that is stored and released along with peptide hormones and neurotransmitters from several tissues, although its exact function is not known. A cDNA (gene symbol CHGA) clone was used as a probe in Southern blot analyses of human-rodent somatic cell hybrid DNAs. Discordancy analysis allowed confirmation of the assignment of the gene to chromosome 14. These results were extended using in situ chromosome hybridization, and a signal was found at 14q32. BglII digestion of genomic DNA from 28 unrelated Caucasian individuals probed with CHGA detected a two-allele RFLP with allelic frequencies of .34 and .66.
authors.affiliation: Biological Carcinogenesis and Development Program, National Cancer Institute-Frederick Research Facility, MD 21701.
authors.forename: W S
authors.initials: WS
authors.lastname: Modi
authors.forename: M A
authors.initials: MA
authors.lastname: Levine
authors.forename: H N
authors.initials: HN
authors.lastname: Seuanez
authors.forename: M
authors.initials: M
authors.lastname: Dean
authors.forename: S J
authors.initials: SJ
authors.lastname: O'Brien
mesh_terms: D015139:Blotting, Southern; D053379:Chromogranin A; D002864:Chromogranins / Q000235:genetics*; D002874:Chromosome Mapping; D002883:Chromosomes, Human, Pair 14; D004247:DNA / Q000235:genetics; D006801:Humans; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010375:Pedigree; D012150:Polymorphism, Restriction Fragment Length
chemical_list: C506150:CHGA protein, human; D053379:Chromogranin A; D002864:Chromogranins; D009419:Nerve Tissue Proteins; D004247:DNA