[Antigenic structure of foot-and-mouth virus. IV. Synthesis and immunogenic properties of new fragments of the VP1 protein of food-and-mouth virus strain A22].
A synthesis of new fragments of VP1 protein with the specificity of A22 strain of foot-and-mouth disease virus is described. Immunization with the free 136-152 peptide and KLH-conjugates of the peptides 136-152 and 197-213 induced 60-80% protection of guinea pigs against challenge with the A22 virus. Synthetic peptides corresponding to the 10-24, 50-69 and 175-189 sequences of VP1 did not show any protective activity. We have found that uncoupled peptides 175-189 and 197-213 are able to induce antipeptide antibodies. However, these antibodies did not possess any neutralizing activity. Immunization of animals with the mixture of (136-152)O1K and (175-189)A22 has led to inhibition of the immune response to the (136-152)O1K fragment.
Iarov
A V
AV
Gel'fanov
V M
VM
Grechaninova
L A
LA
Surovoĭ
A Iu
AIu
Vol'pina
O M
OM
Ivanov
V T
VT
Chepurkin
A V
AV
Lugovskoĭ
A A
AA
Driagalin
N N
NN
Ivaniushchenkov
V N
VN
D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D005537:Aphthovirus / Q000276:immunology*; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D006168:Guinea Pigs; D008969:Molecular Sequence Data; D014764:Viral Proteins / Q000138:chemical synthesis* / Q000276:immunology
D000956:Antigens, Viral; D014764:Viral Proteins
Antigennaia struktura virusa iashchura. IV. Sintez i immunogennye svoĭstva novykh fragmentov belka VP1 virusa iashchura shtama A22.