Involvement of a cis-element that binds an H2TF-1/NF kappa B like factor(s) in the virus-induced interferon-beta gene expression.
Interferon-beta (IFN-beta) gene is transcriptionally activated following virus infection of various cell types such as fibroblasts. In the previous studies, regulatory DNA sequences that mediate the virus-induced transcriptional activation have been identified within the 5'-flanking region (up to around -117 respect to the CAP site) of the human IFN-beta gene. The sequences contain binding sites (-100 to -61) for a transcriptional activator, IRF-1, the gene of which is also virus-inducible. In the present study, we focused on an additional cis-element, located between the IRF-1 binding sites and TATA box. Interestingly, the element coincides with the previously identified elements for the transcription factors H2TF-1 and NF kappa B. The element, when tandemly repeated, functions in activating the distal gene expression in either constitutive or virus-inducible manner depending on the cell type. The results suggest the importance of cooperation between IRF-1 and H2TF-1/NF kappa B-like factor in the maximal IFN-beta gene induction.
Fujita
T
T
Institute for Molecular and Cellular Biology, Osaka University, Japan.
Miyamoto
M
M
Kimura
Y
Y
Hammer
J
J
Taniguchi
T
T
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002472:Cell Transformation, Viral; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D005786:Gene Expression Regulation; D005796:Genes; D005801:Genes, Homeobox; D005809:Genes, Regulator; D006801:Humans; D007369:Interferon Inducers; D007370:Interferon Type I / Q000235:genetics*; D008969:Molecular Sequence Data; D009522:Newcastle disease virus / Q000235:genetics*; D010957:Plasmids; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D014162:Transfection
D007369:Interferon Inducers; D007370:Interferon Type I; D015500:Chloramphenicol O-Acetyltransferase