Release of superoxide-dependent relaxing factor(s) from endothelial cells.
In the present work, an experimental system was designed to study superoxide anion radical, implicated as the cause of vascular dilatation. To circumvent its direct effect, we employed a two-bath system. When the endothelial cells (EC) were exposed to electrical field stimulation (EFS) or to a hypoxanthine-xanthine oxidase system in bath A plus its physiological buffer solution suffused on a helical strip of cat basilar artery in bath B, the contraction to 5-hydroxytryptamine (5-HT) was depressed to approximately 40-50% of the control value. The reduction was not elicited on EFS in a state of calcium deficiency or in the absence of EC. The depression could be prevented by pretreatment with superoxide dismutase (SOD), but not with an effective dose of catalase, dimethyl sulfoxide (DMSO), mannitol, or indomethacin. The percent depression of contraction was paralleled by an increase in SOD-inhibitable cytochrome c reduction, which was not associated with cyclic guanosine 3',5'-monophosphate formation. These results suggest that superoxide-dependent relaxing factor is released from EC differently than the endothelium-derived relaxing factor mediated by acetylcholine.
Hong
K W
KW
Department of Pharmacology, College of Medicine, Pusan National University, Korea.
Rhim
B Y
BY
Lee
W S
WS
Jeong
B R
BR
Kim
C D
CD
Shin
Y W
YW
D000818:Animals; D000838:Anions / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D006152:Cyclic GMP / Q000378:metabolism; D004558:Electric Stimulation; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism; D005609:Free Radicals; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*; D014969:Xanthine Oxidase / Q000494:pharmacology
D000838:Anions; D005609:Free Radicals; D007042:Hypoxanthines; D013481:Superoxides; D019271:Hypoxanthine; D009569:Nitric Oxide; D014969:Xanthine Oxidase; D006152:Cyclic GMP; D010100:Oxygen; D002118:Calcium