title: Electrophoretic mobility of gamma-glutamyltransferase in rat liver subcellular fractions. Evidence for structure difference from the kidney enzyme.
abstract: Adult rat liver gamma-glutamyltransferase (GGT) has been poorly characterized because of its very low concentration in the tissue. In contrast with the kidney, the liver enzyme is inducible by some xenobiotics, and its relationship to hepatic ontogeny and carcinogenesis seems to be important. Liver GGT polypeptides were identified by immunoblot analysis in subcellular fractions (rough endoplasmic reticulum, smooth endoplasmic reticulum, Golgi membranes and plasma membranes). Rat liver GGT appeared as a series of polypeptides corresponding to different maturation steps. Polypeptides related to the heavy subunit of GGT were detected in rough endoplasmic reticulum at 49, 53 and 55 kDa, and in Golgi membranes at 55, 60 and 66 kDa. Two polypeptides related to the light subunit of GGT were also observed in Golgi membranes. In plasma membranes GGT was composed of 100 kDa, 66 kDa and 31 kDa polypeptides. The 66 kDa component could correspond to the heavy subunit of the rat liver enzyme, and if so has a molecular mass higher than that of the purified rat kidney form of GGT (papain-treated). These data suggest different peptide backbones for the heavy subunits of liver GGT and kidney GGT.
authors.affiliation: Centre du Médicament, U.R.A. C.N.R.S. 597, Nancy, France.
authors.forename: B
authors.initials: B
authors.lastname: Antoine
authors.forename: A
authors.initials: A
authors.lastname: Visvikis
authors.forename: C
authors.initials: C
authors.lastname: Thioudellet
authors.forename: A
authors.initials: A
authors.lastname: Rahimi-Pour
authors.forename: N
authors.initials: N
authors.lastname: Strazielle
authors.forename: M
authors.initials: M
authors.lastname: Wellman
authors.forename: G
authors.initials: G
authors.lastname: Siest
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002462:Cell Membrane / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D004721:Endoplasmic Reticulum / Q000201:enzymology; D006056:Golgi Apparatus / Q000201:enzymology; D008099:Liver / Q000201:enzymology* / Q000648:ultrastructure; D008297:Male; D015394:Molecular Structure; D051381:Rats; D011919:Rats, Inbred Strains; D005723:gamma-Glutamyltransferase
chemical_list: D005723:gamma-Glutamyltransferase