In vivo and in vitro metabolism of benzo(a)pyrene by the larva of the newt, Pleurodeles waltl.
1. The larva of the amphibian species, Pleurodeles waltl was shown to metabolize benzo(a)pyrene in vivo into a variety of oxidized products. 2. In vitro, BaP hydroxylase (AHH) activity was found in hepatic microsomes and postmitochondrial fractions from both larvae and adults of the pleurodele. 3. The clastogenic effect of BaP formation of micronuclei in the erythrocytes was shown to be related to the presence of BaP quinones in the tissues of the newt.
Marty
J
J
Centre de Biologie du Développement, Université Paul Sabatier, Toulouse, France.
Lesca
P
P
Jaylet
A
A
Ardourel
C
C
Rivière
J L
JL
D000818:Animals; D001564:Benzo(a)pyrene / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D066298:In Vitro Techniques; D007814:Larva / Q000378:metabolism; D015162:Micronucleus Tests; D008862:Microsomes, Liver / Q000378:metabolism; D010999:Pleurodeles / Q000378:metabolism*; D012455:Salamandridae / Q000378:metabolism*
D001564:Benzo(a)pyrene