title: Inactivation properties of T-type calcium current in canine cardiac Purkinje cells.
abstract: The kinetic behavior of T-type Ca2+ current (ICa-T) was studied in canine cardiac Purkinje cells using a single suction-pipette whole-cell voltage clamp method. ICa-T was studied without contamination of conventional L-type Ca2+ current (ICa-L). Ca2+, Sr2+, or Ba2+ were used as the charge carrier. During maintained depolarization ICa-T decayed rapidly, and under most conditions the decay showed a voltage-dependent single exponential time course that did not depend on the species of charge carrier. The development of inactivation did not depend on Ca2+, but the time course required more than a single exponential process. Just negative to the threshold voltage for activating ICa-T, inactivation slowly developed and there was a delay in its onset. The time course of recovery from inactivation was dependent on the protocol used to measure it. As the duration of an inactivating voltage step was increased, recovery slowed markedly and there was a delay in its onset. The time course of recovery could be fit as a biexponential. The fast and slow time constants of recovery were relatively constant, however, the relative amplitudes were dependent on the duration of the inactivating voltage step. Recovery was not dependent on Ca2+, and it was slower at a less negative voltage. These results suggest that the T-type Ca2+ channel in cardiac Purkinje cells follows a complex kinetic scheme dependent only on voltage. This behavior can be accounted for by incorporating into a Markovian model several inactivated and closed states.
authors.affiliation: Department of Medicine, University of Chicago, Illinois 60637.
authors.forename: Y
authors.initials: Y
authors.lastname: Hirano
authors.forename: H A
authors.initials: HA
authors.lastname: Fozzard
authors.forename: C T
authors.initials: CT
authors.lastname: January
mesh_terms: D000818:Animals; D001464:Barium / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D015220:Calcium Channels / Q000187:drug effects / Q000502:physiology*; D004285:Dogs; D004594:Electrophysiology / Q000379:methods; D066298:In Vitro Techniques; D007700:Kinetics; D008564:Membrane Potentials / Q000187:drug effects; D008954:Models, Biological; D011689:Purkinje Cells / Q000502:physiology*; D015222:Sodium Channels / Q000502:physiology
chemical_list: D015220:Calcium Channels; D015222:Sodium Channels; D001464:Barium; D002118:Calcium