A rapid procedure to identify newborn transgenic mice.
We have developed a rapid procedure to identify newborn transgenic mice containing foreign genetic material in their genome. The protocol involves collagenase digestion of a small amount of tail tissue which can be taken very early after birth, phenol and chloroform extraction, polymerase chain reaction, and polyacrylamide gel electrophoresis. The entire procedure, from tissue biopsy to final results, can be completed in 1 day.
Lin
C S
CS
Department of Genetics, School of Medicine, Case Western Reserve University, Cleveland, OH 44106.
Magnuson
T
T
Samols
D
D
D000818:Animals; D000831:Animals, Newborn / Q000235:genetics*; D015139:Blotting, Southern; D002097:C-Reactive Protein / Q000235:genetics; D004274:DNA, Recombinant / Q000032:analysis*; D004259:DNA-Directed DNA Polymerase; D004591:Electrophoresis, Polyacrylamide Gel; D005821:Genetic Techniques; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics*; D003012:Microbial Collagenase
D004274:DNA, Recombinant; D002097:C-Reactive Protein; D004259:DNA-Directed DNA Polymerase; D003012:Microbial Collagenase