Gene UL11 of herpes simplex virus type 1 encodes a virion protein which is myristylated.
We have investigated whether herpes simplex virus (HSV) contains structural polypeptides which are modified by myristic acid. We demonstrate that herpes simplex virions contain a family of myristylated proteins, Mr approximately 13,000 to 16,000. These were mapped, using HSV-1/HSV-2 intertypic recombinants, to 0.130 to 0.204 map units on the virus genome. Using anti-peptide sera, raised against the carboxyterminus of the predicted UL11 gene product, we have established that the myristylated virion polypeptides are products of the viral gene UL11.
MacLean
C A
CA
MRC Virology Unit, University of Glasgow, U.K.
Clark
B
B
McGeoch
D J
DJ
D004279:DNA, Viral / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral; D009227:Myristic Acids / Q000378:metabolism*; D011233:Precipitin Tests; D015183:Restriction Mapping; D018139:Simplexvirus / Q000235:genetics* / Q000378:metabolism; D015678:Viral Structural Proteins / Q000235:genetics* / Q000378:metabolism; D014771:Virion / Q000235:genetics / Q000378:metabolism
D004279:DNA, Viral; D009227:Myristic Acids; D015678:Viral Structural Proteins