Procollagenase associated with the noncalcified matrix of bone and its regulation by parathyroid hormone.
Collagenolytic enzyme activity associated with the noncalcified pool of collagen was studied using calvarial matrices from which the periosteal cell envelope had been removed. Aminophenylmercuric acetate (APMA) stimulated degradation of about 5% of the noncalcified collagen in matrices prepared from freshly dissected bone. Significantly more activity was detected if intact calvaria were cultured 24 h before removal of the cells, in which case 20-30% of the noncalcified collagen was degraded following treatment with APMA. Trypsin elicited a similar response. The collagen being degraded was representative of the entire pool of noncalcified collagen and was not underhydroxylated. Treatment of intact calvaria with parathyroid hormone (PTH) before removal of the cells increased the level of both active collagenase and procollagenase activity associated with the matrix. Enhanced 3H release was noted for PTH treated intact bone in the prior 24 h. Inactivation of endogenous procollagenase by phenanthroline had no effect on the ability of isolated calvarial cells to resorb the bone upon treatment with resorptive agents. The data show that PTH-stimulated collagenolysis of noncalcified collagen involves increased deposition of procollagenase onto the noncalcified matrix in addition to activation of the enzyme.
Jilka
R L
RL
Research Service, Veterans Administration Medical Center, Kansas City, Missouri 64128.
D000818:Animals; D001857:Bone Matrix / Q000201:enzymology*; D001862:Bone Resorption / Q000378:metabolism; D017364:Collagenases; D004792:Enzyme Precursors / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006909:Hydroxyproline / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D003012:Microbial Collagenase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010281:Parathyroid Hormone / Q000502:physiology*; D010618:Phenanthrolines / Q000494:pharmacology; D010662:Phenylmercuric Acetate / Q000031:analogs & derivatives / Q000494:pharmacology; D014357:Trypsin
D004792:Enzyme Precursors; D010281:Parathyroid Hormone; D010618:Phenanthrolines; C010024:4-aminophenylmercuriacetate; D014357:Trypsin; D017364:Collagenases; C022440:procollagenase; D003012:Microbial Collagenase; D010662:Phenylmercuric Acetate; D006909:Hydroxyproline