Radioimmunoimaging of lung vessels: an approach using indium-111-labeled monoclonal antibody to angiotensin-converting enzyme.
A murine monoclonal antibody against human angiotensin-converting enzyme was radiolabeled with 111In via diethylenetriaminepentaacetic acid without substantial loss of antigen-binding capacity. This monoclonal antibody designated 9B9 cross-reacted with rat and monkey angiotensin-converting enzyme. Indium-111-labeled 9B9 selectively accumulated 10-20 times greater in the lung than in blood or other organs following intravenous administration in rats. Kinetics of lung accumulation and blood clearance were studied for 111In-9B9-antibody and compared to that of 125I-labeled 9B9 in rat. Highly specific accumulation of 111In-9B9-antibody in the lung of Macaca Rhesus monkeys after intravenous injection was monitored by gamma-imaging. Images of 111In-labeled antibody 9B9 biodistribution in monkey lung noticeably differ from the images of biodistribution of 99mTc-labeled albumin microspheres. This difference may provide information concerning the state of the endothelium of lung capillaries, which is different from the blood flow characteristics determined with routine microsphere technique.
Danilov
S M
SM
Institute of Experimental Cardiology, USSR Cardiology Research Center, Moscow.
Martynov
A V
AV
Klibanov
A L
AL
Slinkin
M A
MA
Sakharov IYu
Malov
A G
AG
Sergienko
V B
VB
Vedernikov AYu
Muzykantov
V R
VR
Torchilin
V P
VP
D000818:Animals; D000911:Antibodies, Monoclonal; D007205:Indium Radioisotopes / Q000493:pharmacokinetics; D008168:Lung / Q000098:blood supply / Q000000981:diagnostic imaging*; D008253:Macaca mulatta; D007703:Peptidyl-Dipeptidase A / Q000276:immunology*; D011877:Radionuclide Imaging; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D007205:Indium Radioisotopes; D007703:Peptidyl-Dipeptidase A