title: Redistribution of subcellular calcium in rat liver on administration of vanadate.
abstract: Mitochondria isolated from the livers of rats administered with sodium meta-, ortho-, or polyvanadate, but not vanadyl sulphate, exhibited enhanced Ca2+ -stimulated respiration and uptake of calcium. These effects were shown also by mitochondria isolated from livers perfused with polyvanadate. The concentration of acid-soluble calcium decreased significantly in the mitochondrial fraction on vanadate treatment, while that in the cytosol showed a corresponding increase. Phenoxybenzamine, an antagonist to alpha-adrenergic receptors, effectively inhibited vanadate-induced Ca2+ mobilization, but surgical sympathectomy was without effect. This is the first demonstration of vanadate mimicking alpha-adrenergic agonists in vivo.
authors.affiliation: Department of Biochemistry, Indian Institute of Science, Bangalore.
authors.forename: S
authors.initials: S
authors.lastname: Gullapalli
authors.forename: V
authors.initials: V
authors.lastname: Shivaswamy
authors.forename: T
authors.initials: T
authors.lastname: Ramasarma
authors.forename: C K
authors.initials: CK
authors.lastname: Kurup
mesh_terms: D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D010477:Perfusion; D011768:Pyruvate Dehydrogenase Complex / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012119:Respiration; D013347:Subcellular Fractions / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D014638:Vanadates / Q000494:pharmacology*
chemical_list: D000317:Adrenergic alpha-Antagonists; D011768:Pyruvate Dehydrogenase Complex; D014638:Vanadates; D002118:Calcium