Rearranged Epstein-Barr virus genomes and clonal origin in nasopharyngeal carcinoma.
Epstein-Barr virus (EBV) is known to be associated with two malignant diseases, nasopharyngeal carcinoma (NPC) and endemic Burkitt's lymphoma. In this study, the genomes of EBV in biopsy specimens from 4 NPC patients in Japan were analyzed using Southern blot hybridization. The NPC tissues of all examined cases contained rearranged EBV genomes whose BamHI H fragments were larger than those of prototype EBV genomes. One of them had a BamHI fragment containing contiguous sequences of BamHI Y and H. A single-sized EBV DNA terminus was observed in these NPC tissues, implying the evolution of the carcinoma from a single EBV-infected cell.
Shimakage
M
M
Clinical Research Institute, Osaka National Hospital.
Chatani
M
M
Ikegami
N
N
Hirai
K
K
D000914:Antibodies, Viral / Q000032:analysis; D001706:Biopsy; D015139:Blotting, Southern; D002277:Carcinoma / Q000235:genetics / Q000382:microbiology* / Q000473:pathology; D002999:Clone Cells; D004273:DNA, Neoplasm / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000382:microbiology* / Q000473:pathology
D000914:Antibodies, Viral; D004273:DNA, Neoplasm; D004279:DNA, Viral