[The effect of cobalt on the dynamics of membrane-bound calcium in the cerebral cortex structures].
Cobalt (Co2+) influence on calcium bound (Cab2+) dynamics detected by a fluorescent chlorotetracyclin probe (CTC), as well as bioelectric activity were studied in the feline vital brain cortex preparation with contact microscopy and microelectrode technique. It has been found that Co2+ applied on the preparation surface or injected ionophoretically into microzones under microscopy produced a distinct decrease of CTC-Cab2+ fluorescence intensity. This indicates a decrease of Cab2+ content in the hydrophobic membrane loci. The effect is reversible and depends on Co2+ concentration. Neuronal excitation appeared after the fluorescence decrease up to 5-12% to the start level. It has been concluded that Cab2+ content dynamics plays an important role in the Co2+ "seizure" discharges mechanisms in the cortex.
Samoĭlov
M O
MO
Semenov
D G
DG
Cherniatchik
G Iu
GIu
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000187:drug effects*; D002415:Cats; D002462:Cell Membrane / Q000378:metabolism / Q000502:physiology; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D003035:Cobalt / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects; D013050:Spectrometry, Fluorescence
D015220:Calcium Channels; D003035:Cobalt; D002118:Calcium
Vliianie kobal'ta na dinamiku soderzhaniia membranosviazannogo kal'tsiia v strukturakh kory golovnogo mozga.