The dephosphorylation of lens alpha-crystallin A chain.
The present communication reports the presence of a phosphoprotein phosphatase activity in bovine lens preparations which dephosphorylates alpha Ap, the phosphorylated form of alpha A, one of the alpha-crystallin polypeptides, in a Ca2+/calmodulin dependent manner. The activity was found in soluble preparations from epithelial cells but it could not be detected in similar preparations from fiber cells. A 60,000 Mr calmodulin binding polypeptide and a 15,000 Mr polypeptide found in the epithelial cell preparations comigrated in SDS-PAGE with the A and B subunits of bovine brain calcineurin (phosphoprotein phosphatase 2B) respectively. The 15,000 Mr was specifically recognized by an anti-bovine brain calcineurin antiserum. Bovine brain calcineurin was as effective in dephosphorylating alpha Ap as the lens preparations. Thus, it is likely that the activity present in the lens is related to this enzyme. The results indicate that the lens specific polypeptide alpha A may be subject to metabolic control through phosphorylation and dephosphorylation pathways regulated by cAMP and calcium respectively. Changes in the activities of these pathways appear to occur during differentiation of the lens epithelial cell and may be related to gene regulation during the differentiation process.
Chiesa
R
R
Department of Ophthalmology, College of Physicians and Surgeons of Columbia University, New York, NY 10032.
Spector
A
A
D000818:Animals; D019703:Calcineurin; D002118:Calcium / Q000502:physiology; D002147:Calmodulin / Q000502:physiology; D002148:Calmodulin-Binding Proteins / Q000502:physiology; D002417:Cattle; D002454:Cell Differentiation; D003459:Crystallins / Q000378:metabolism*; D004848:Epithelium / Q000201:enzymology; D066298:In Vitro Techniques; D007908:Lens, Crystalline / Q000166:cytology / Q000378:metabolism*; D008970:Molecular Weight; D010749:Phosphoprotein Phosphatases / Q000378:metabolism* / Q000502:physiology; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational
D002147:Calmodulin; D002148:Calmodulin-Binding Proteins; D003459:Crystallins; D019703:Calcineurin; D010749:Phosphoprotein Phosphatases; D002118:Calcium