Inhibition of adenylate kinase by P1,P5-di(adenosine 5') pentaphosphate in assays of erythrocyte enzyme activities requiring adenine nucleotides.
P1,P5-di(adenosine 5')pentaphosphate (Ap5A) is an excellent inhibitor of human hemolysate adenylate kinase at concentrations near 2 microM and above. At ten times this concentration and in hemolysate enzyme assays under conditions described in this paper it appears not to alter reaction data in the case of hexokinase, phosphofructokinase, and phosphoglycerokinase. In the pyruvate kinase assay, very modest reductions in activity are noted, and kinetics with phosphoenolpyruvate, adenosine diphosphate (ADP), and uridine diphosphate (UDP) are unaltered.
Valentine
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Department of Medicine, University of California Center for the Health Sciences, Los Angeles 90024.
Paglia
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DE
Nakatani
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Brockway
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D000244:Adenosine Diphosphate; D000263:Adenylate Kinase / Q000037:antagonists & inhibitors*; D015226:Dinucleoside Phosphates / Q000494:pharmacology*; D004912:Erythrocytes / Q000201:enzymology*; D006593:Hexokinase / Q000037:antagonists & inhibitors; D006801:Humans; D010728:Phosphoenolpyruvate; D010732:Phosphofructokinase-1 / Q000037:antagonists & inhibitors; D010735:Phosphoglycerate Kinase / Q000037:antagonists & inhibitors; D010770:Phosphotransferases / Q000037:antagonists & inhibitors*; D011770:Pyruvate Kinase / Q000037:antagonists & inhibitors; D014530:Uridine Diphosphate
D015226:Dinucleoside Phosphates; C012275:P(1),P(5)-di(adenosine-5'-)pentaphosphate; D014530:Uridine Diphosphate; D000244:Adenosine Diphosphate; D010728:Phosphoenolpyruvate; D010770:Phosphotransferases; D006593:Hexokinase; D010732:Phosphofructokinase-1; D011770:Pyruvate Kinase; D010735:Phosphoglycerate Kinase; D000263:Adenylate Kinase