title: Detection of leukotrienes B4, C4 and of their isomers in arterial, mixed venous blood and bronchoalveolar lavage fluid from ARDS patients.
abstract: Seven patients with the adult respiratory distress syndrome (ARDS) were studied. As a control group we used 6 surgical patients who underwent minor surgical operation (inguinal hernia). For both groups the same sample collection and analysis was used. The presence of leuktorienes (LTs) B4 and C4 and of their isomers 11-trans LTC4 and delta 6-trans-12-epi LTB4 was determined in arterial, mixed venous blood and in bronchoalveolar lavage (BAL) fluid. The samples, analysed by reverse phase high performance liquid chromatography (RP-HPLC), showed a similar chromatographic picture among ARDS patients, while the control group showed no detectable amounts of LTs in BAL or blood. The distribution of these arachidonic acid metabolites in mixed venous blood, arterial blood and BAL seems to suggest pulmonary metabolism and/or inactivation. It is suggested that these mediators act as humoral factors in pathogenesis of the ARDS.
authors.affiliation: Institute of Anaesthesiology and Intensive Care, University La Sapienza Rome, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Antonelli
authors.forename: M
authors.initials: M
authors.lastname: Bufi
authors.forename: R A
authors.initials: RA
authors.lastname: De Blasi
authors.forename: G
authors.initials: G
authors.lastname: Crimi
authors.forename: G
authors.initials: G
authors.lastname: Conti
authors.forename: C
authors.initials: C
authors.lastname: Mattia
authors.forename: G
authors.initials: G
authors.lastname: Vivino
authors.forename: L
authors.initials: L
authors.lastname: Lenti
authors.forename: D
authors.initials: D
authors.lastname: Lombardi
authors.forename: A
authors.initials: A
authors.lastname: Dotta
mesh_terms: D000328:Adult; D001992:Bronchoalveolar Lavage Fluid / Q000032:analysis*; D005260:Female; D006801:Humans; D007975:Leukotriene B4 / Q000032:analysis / Q000097:blood* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D012128:Respiratory Distress Syndrome / Q000097:blood / Q000175:diagnosis* / Q000378:metabolism; D013189:SRS-A / Q000032:analysis / Q000097:blood* / Q000378:metabolism
chemical_list: D013189:SRS-A; D007975:Leukotriene B4