Immunodiagnosis of sleeping sickness due to Trypanosoma brucei gambiense by detection of antiprocyclic antibodies and trypanosome antigens in patients' sera.
Documented sera from 39 Trypanosoma brucei gambiense sleeping sickness patients from Ivory Coast (Côte d'Ivoire) were tested using the Procyclic Agglutination Trypanosomiasis Test (PATT) for the presence of anti-trypanosome antibodies and using an antigen-capture double antibody enzyme-linked immunosorbent assay (ELISA) for the presence of trypanosomal antigens. All 39 sera contained antiprocyclic antibodies and trypanosome antigens whereas 5 control sera did not. The results show that the PATT (for antibody detection) and the double antibody ELISA (for antigen detection) are useful for immunodiagnosis of African sleeping sickness due to T.b. gambiense and that these assays should be simplified for further testing and evaluation in the field.
Liu
M K
MK
Department of Biochemistry and Microbiology, University of Victoria, British Columbia.
Cattand
P
P
Gardiner
I C
IC
Pearson
T W
TW
D000372:Agglutination Tests; D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis*; D000953:Antigens, Protozoan / Q000032:analysis*; D007560:Cote d'Ivoire; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D014347:Trypanosoma brucei gambiense / Q000276:immunology*; D014353:Trypanosomiasis, African / Q000134:cerebrospinal fluid / Q000175:diagnosis*
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan