Protein kinase C: a new linkage marker for growth hormone and for COL1A1.
An expanded linkage group on the long arm of human chromosome 17 is reported. Using the CEPH panel of DNAs and restriction fragment length polymorphism (RFLP) markers for the centromere locus (D17Z1), growth hormone (GH1), collagen type I alpha 1 (COL1A1), and protein kinase C-alpha polypeptide (PKCA) loci, theta values of 0.03, 0.11, and 0.23 were found between PKCA and GH1, PKCA and COL1A1, and PKCA and D17Z1, respectively. The theta values calculated for GH1 versus COL1A1 or D17Z1 were 0.11 and 0.23, respectively. Sex-specific recombination rates were calculated for the best likelihood order and demonstrate female recombination greater than male recombination. Therefore, the loci studied span a map region of approximately 30 cm between 17cen and 17q24, with the most likely gene order being D17Z1-COL1A1-PKCA-GH1.
Summar
M L
ML
Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
Phillips
J A
JA
Krishnamani
M R
MR
Keefer
J
J
Trofatter
J
J
Schwartz
R C
RC
Tsipouras
P
P
Willard
H
H
Ullrich
A
A
D000483:Alleles; D002503:Centromere / Q000032:analysis; D002874:Chromosome Mapping; D002886:Chromosomes, Human, Pair 17; D003094:Collagen / Q000235:genetics*; D000090602:Collagen Type I, alpha 1 Chain; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D003627:Data Interpretation, Statistical; D005787:Gene Frequency; D008040:Genetic Linkage; D005819:Genetic Markers / Q000032:analysis; D013006:Growth Hormone / Q000235:genetics*; D006801:Humans; D008126:Lod Score; D012150:Polymorphism, Restriction Fragment Length; D011493:Protein Kinase C / Q000235:genetics*
C000716127:COL1A1 protein, human; D000090602:Collagen Type I, alpha 1 Chain; D015342:DNA Probes; D005819:Genetic Markers; D013006:Growth Hormone; D003094:Collagen; D004247:DNA; D011493:Protein Kinase C