Mechanism of spontaneous loss of heat-stable toxin (STa) production in enterotoxigenic Escherichia coli.
Five strains of enterotoxigenic Escherichia coli (ETEC) showing spontaneous loss of heat-stable enterotoxin (STa) production were studied to elucidate the underlying genetic mechanisms. Southern blot analysis revealed that loss of STa production, and the corresponding lack of hybridization with the STa gene probes, were associated with deletions of DNA fragments harboring the relevant toxin genes rather than with loss of plasmids.
Sommerfelt
H
H
Medical Department B, University of Bergen, Haukeland Hospital, Norway.
Haukanes
B I
BI
Kalland
K H
KH
Svennerholm
A M
AM
Sanchéz
J
J
Bjorvatn
B
B
D000942:Antigens, Bacterial / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics*; D015139:Blotting, Southern; D015342:DNA Probes; D004768:Enterotoxins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000472:pathogenicity; D029968:Escherichia coli Proteins; D010861:Fimbriae, Bacterial; D005798:Genes, Bacterial; D010957:Plasmids
D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D015342:DNA Probes; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C034151:heat stable toxin (E coli)