The interaction of fructose-1,6-biphosphate aldolase with liposome membranes: a spin probe technique study.
Thermotropic properties of liposome membranes prepared of bulk bovine erythrocyte membrane lipids, native, or aldolase-modified, were investigated by the ESR method. Breaks were observed in the log 2T parallel vs 1/T plots for two spin labels: tempopalmitate and 5-doxyl-palmitate methyl ester. These phenomena have been interpreted as reflecting structural changes near the lipid bilayer polar heads region. Upon modification with aldolase, the temperature at which the breaks occurred was decreased for both spin probes.
Komorowska
M
M
Institute of Physics, Technical University of Wroclaw, Poland.
Langner
M
M
Gomułkiewicz
J
J
D000818:Animals; D002417:Cattle; D004578:Electron Spin Resonance Spectroscopy; D004910:Erythrocyte Membrane / Q000378:metabolism; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism*; D007700:Kinetics; D008081:Liposomes; D008563:Membrane Lipids / Q000097:blood; D013113:Spin Labels; D013816:Thermodynamics
D008081:Liposomes; D008563:Membrane Lipids; D013113:Spin Labels; D005634:Fructose-Bisphosphate Aldolase