title: Indirect spectrophotometric determination of BIDA, DISIDA, DTPA and MDP in labelled compounds.
abstract: N-(4-(n-butyl)-acetanilide)iminodiacetic acid (BIDA), N-(2,6-diisopropylacetanilide)iminodiacetic acid (DISIDA), diethylenetriaminepentaacetic acid (DTPA) and methylene diphosphonic acid (MDP) are used in labelling kits. The contents of BIDA, DISIDA or MDP of the 99mTc-labelled compounds can be determined (indirectly) spectrophotometrically with copper, eriochrome cyaanine R (ECC) and dodecylethyldimethylammonium bromide (DEDA) in a sodium barbital buffered system at pH 8.5. The calibration curves obey Beer's Law from 0 to 40 micrograms/25 mL for BIDA and DISIDA, 0 to 60 micrograms/25 mL for DTPA and 0 to 100 micrograms/10 mL for MDP.
authors.affiliation: Department of Chemistry, Rand Afrikaans University, Johannesburg, Republic of South Africa.
authors.forename: T N
authors.initials: TN
authors.lastname: van der Walt
authors.forename: P P
authors.initials: PP
authors.lastname: Coetzee
authors.forename: P J
authors.initials: PJ
authors.lastname: Fourie
mesh_terms: D002138:Calibration; D007098:Imino Acids / Q000032:analysis*; D015609:Organotechnetium Compounds; D004369:Pentetic Acid / Q000032:analysis*; D011933:Reagent Kits, Diagnostic; D013053:Spectrophotometry / Q000379:methods; D013667:Technetium / Q000032:analysis*; D019785:Technetium Tc 99m Disofenin; D013669:Technetium Tc 99m Medronate / Q000032:analysis*; D016284:Technetium Tc 99m Pentetate
chemical_list: D007098:Imino Acids; D015609:Organotechnetium Compounds; D011933:Reagent Kits, Diagnostic; C024340:technetium Tc 99m (para-butyl)iminodiacetic acid; D013667:Technetium; D004369:Pentetic Acid; D019785:Technetium Tc 99m Disofenin; D016284:Technetium Tc 99m Pentetate; D013669:Technetium Tc 99m Medronate