OKT4 monoclonal antibody-induced activation of an autoreactive T-cell clone.
We report the presence of a CD4-mediated T-cell activation pathway on an autoreactive CD3+WT31+CD4+CD8- T-cell clone, designated 2F9, isolated from the peripheral blood of a patient with ovarian adenocarcinoma. The OKT4 mab modulated the CD4 antigen independently of the CD3 antigen or the alpha beta T-cell receptor. OKT4 mab immobilized on plastic or soluble OKT4 mab in the presence of feeder PBMC induced proliferation and IL-2 production by cells of the 2F9 clone. Mixtures of the OKT4 mab and the OKT3 or anti-WT31 mabs induced additive proliferative responses and IL-2 production. The OKT4 mab synergized with recombinant IL-2 in inducing proliferative responses. These results suggest the presence of activation pathway on 2F9 cells.
Ioannides
C G
CG
Department of Gynecology, University of Texas, Houston 77030.
Freedman
R S
RS
Platsoucas
C D
CD
D000230:Adenocarcinoma / Q000276:immunology; D000276:Adjuvants, Immunologic / Q000502:physiology; D000911:Antibodies, Monoclonal / Q000502:physiology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002999:Clone Cells / Q000145:classification / Q000276:immunology; D005260:Female; D006801:Humans; D008213:Lymphocyte Activation; D010051:Ovarian Neoplasms / Q000276:immunology; D010641:Phenotype; D014407:Tumor Cells, Cultured
D000276:Adjuvants, Immunologic; D000911:Antibodies, Monoclonal