The cDNA of the two isoforms of bovine cGMP-dependent protein kinase.
cDNAs encoding the isoform I alpha of the cGMP-dependent protein kinase were isolated from a bovine trachea smooth muscle cDNA library constructed in lambda gt10. The deduced protein sequence is identical with the protein sequence obtained by Edman degradation of the bovine lung enzyme [(1984) Biochemistry 23, 4207-4218]. Alternate cDNA clones were isolated which code for a protein slightly different within the aminoterminal part from the known amino acid sequence. These alternate cDNAs contain the sequence of a peptide identified in the isoform I beta of cGMP-dependent protein kinase. Northern blot analysis of poly(A)+ RNA from bovine trachea smooth muscle indicated the presence of two different mRNA species of about 6.2 kb.
Wernet
W
W
Institut für Physiologische Chemie, Medizinische Fakultät, Universität des Saarlandes, Homburg/Saar, FRG.
Flockerzi
V
V
Hofmann
F
F
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D006152:Cyclic GMP / Q000494:pharmacology*; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D015342:DNA Probes; D007527:Isoenzymes / Q000235:genetics*; D008969:Molecular Sequence Data; D009130:Muscle, Smooth / Q000201:enzymology; D009693:Nucleic Acid Hybridization; D011494:Protein Kinases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D014132:Trachea / Q000201:enzymology
D015342:DNA Probes; D007527:Isoenzymes; D012333:RNA, Messenger; D004247:DNA; D011494:Protein Kinases; D006152:Cyclic GMP