Retention and expression of the left end subfragment of the herpes simplex virus type 2 BglII N DNA fragment do not correlate with tumorigenic conversion of NIH 3T3 cells.
Cotransfection experiments have been carried out using recombinant plasmids pAG60, conferring resistance to antibiotic G418, and pXho3 which contains the left end subfragment (map coordinates 0.583 to 0.596) of the transforming herpes simplex virus type 2 BglII N DNA fragment and encodes the 36K polypeptide associated with the viral ribonucleotide reductase activity. Several NIH 3T3 cell clones resistant to G418 and having morphological changes commonly observed for transformed NIH 3T3 cells were isolated and examined for the presence and stable retention of the viral sequences. Seven of the clones that retained the transfected viral sequences were analysed for the expression of the 36K polypeptide and the tumorigenic phenotype. The results gathered from these studies show that neither the retention of the viral DNA nor the expression of the 36K polypeptide correlated with tumorigenic conversion of these cells.
Kessous-Elbaz
A
A
Institut du Cancer de Montréal, Centre Hospitalier Notre-Dame, Québec, Canada.
Pelletier
M
M
Cohen
E A
EA
Langelier
Y
Y
D000818:Animals; D001426:Bacterial Proteins; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000382:microbiology*; D002472:Cell Transformation, Viral; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific; D005347:Fibroblasts / Q000382:microbiology*; D005785:Gene Conversion; D005814:Genes, Viral; D051379:Mice; D018139:Simplexvirus / Q000235:genetics*; D014162:Transfection
D001426:Bacterial Proteins; D004279:DNA, Viral; C024404:BglII endonuclease; D015252:Deoxyribonucleases, Type II Site-Specific