title: Effects of continuous exposure to 239Pu-alpha rays on Co631 cells.
abstract: Exposure of Co631 cells to 239Pu-citrate leads to continuous irradiation and results in increasing cell death which depends on dose and incubation time of the metal. The D37 is calculated to be 0.13 Gy. Plutonium-239 induces the amplification of the integrated SV40 sequences of the Co631 cells. Other cellular genes such as the alpha-actin gene were not amplified, indicating that the SV40-gene amplification is a selective process. Southern blot analysis of genomic DNA shows that not all S40 sequences are equally amplified. Excised sequences of the SV40 genome only appear after an exposure time of 4 days.
authors.affiliation: Kernforschungszentrum Karlsruhe, Institut für Genetik und Toxikologie, F.R.G.
authors.forename: W
authors.initials: W
authors.lastname: Rau
authors.forename: H
authors.initials: H
authors.lastname: Olinger
authors.forename: F
authors.initials: F
authors.lastname: Planas-Bohne
authors.forename: D M
authors.initials: DM
authors.lastname: Taylor
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects*; D002471:Cell Transformation, Neoplastic; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000528:radiation effects; D004279:DNA, Viral / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D011005:Plutonium; D013539:Simian virus 40 / Q000235:genetics
chemical_list: D004279:DNA, Viral; D011005:Plutonium; D004247:DNA