Characterization of pre- and postsynaptic dopamine receptors in Lymnaea.
1. The effects of dopamine and several synthetic agonists and antagonists were studied using two identified neurons of the snail Lymnaea stagnalis. 2. In both the buccal-2 (B-2) neurons and the pedal giant (RPeD1) neuron dopamine elicited a hyperpolarizing response at least partly due to potassium efflux. RPeD1 is itself dopaminergic, implicating autoreceptors in its response to dopamine. 3. The following agents were tested: agonists--LY171555, pergolide, SKF38393, (-)-3-PPP, R(-)NPA and dopamine; antagonists--SCH23390, sulpiride, and metaclopramide. Dibutyryl cAMP was applied to determine whether the response is cAMP-mediated. 4. Results indicate that the pharmacological profiles of dopamine receptors on these neurons are inconsistent with those of either D-1, D-2 or autoreceptors in mammals.
Audesirk
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Biology Department, University of Colorado, Denver 80204.
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D004298:Dopamine / Q000502:physiology; D015259:Dopamine Agents / Q000494:pharmacology; D018492:Dopamine Antagonists; D004873:Ergolines / Q000494:pharmacology; D008195:Lymnaea / Q000032:analysis* / Q000187:drug effects; D009474:Neurons / Q000187:drug effects; D011188:Potassium / Q000378:metabolism; D019257:Quinpirole; D011954:Receptors, Dopamine / Q000032:analysis* / Q000187:drug effects
D015259:Dopamine Agents; D018492:Dopamine Antagonists; D004873:Ergolines; D011954:Receptors, Dopamine; D019257:Quinpirole; D003994:Bucladesine; D011188:Potassium; D004298:Dopamine