Kinetics of heme octapeptide (microperoxidase-8; MP-8) formation studied by high-pressure liquid chromatography (HPLC) monitoring of the peptic and tryptic hydrolysis of horse heart cytochrome-c.
The kinetics of the sequential peptic and tryptic hydrolysis of cytochrome-c to give the heme-peptides microperoxidase-11 (MP-11) and -8 (MP-8), respectively, has been investigated by high performance liquid chromatography (HPLC), and we demonstrate that MP-8 can be prepared from cytochrome-c to the point of lyophilization within 4 hr.
Adams
P A
PA
Department of Chemical Pathology, University of Cape Town Medical School, Republic of South Africa.
Byfield
M P
MP
Goold
R D
RD
Thumser
A E
AE
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003574:Cytochrome c Group / Q000032:analysis*; D005612:Freeze Drying; D006736:Horses; D066298:In Vitro Techniques; D007700:Kinetics; D009206:Myocardium / Q000201:enzymology*; D010434:Pepsin A; D010544:Peroxidases / Q000032:analysis*; D014357:Trypsin
D003574:Cytochrome c Group; D010544:Peroxidases; C021799:microperoxidase; D014357:Trypsin; D010434:Pepsin A