Progesterone production from granulosa cells of individual human follicles derived from diabetic and nondiabetic subjects.
Insulin and insulin-like growth factors have been implicated in the stimulation of ovarian steroidogenesis. To assess the effect of diabetes mellitus on this process, a comparison was made of progesterone production by cultured granulosa cells (50,000 cells/well) from 11 individual follicles of nondiabetic and 6 individual follicles of diabetic women. Diabetic metabolic control was fair [HbA1C 6.8, 8.7 (nl 5.0-7.5)]. Cells were collected by laparoscopic follicular aspiration after ovulation induction and isolated by Percoll gradient centrifugation. Progesterone production was measured after culture with hCG (10 IU/mL) or insulin (100 microU/mL). In both nondiabetic and diabetic groups on day 4, hCG significantly stimulated progesterone production (1,686 +/- 1,268 ng/mL to 4,123 +/- 2,825 ng/mL and 1,059 +/- 249 ng/mL to 1,506 +/- 245 ng/mL, respectively). In nondiabetic follicles, insulin also stimulated progesterone production on days 4 (2366 +/- 1032 ng/mL to 3699 +/- 1582 ng/mL; P less than .05) and 7 (987 +/- 475 ng/mL to 1858 +/- 929 ng/mL; P less than .05); this response was not noted in diabetic granulosa cells. We suggest that insulin-stimulated progesterone production by granulosa cells isolated in the presence of fair diabetic metabolic control is impaired.
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Yale University School of Medicine, Department of Obstetrics and Gynecology, New Haven, Connecticut.
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Polan
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D002478:Cells, Cultured; D003920:Diabetes Mellitus / Q000503:physiopathology*; D005260:Female; D005307:Fertilization in Vitro; D006107:Granulosa Cells / Q000378:metabolism*; D006801:Humans; D007328:Insulin / Q000502:physiology*; D011374:Progesterone / Q000096:biosynthesis*; D013396:Suction
D007328:Insulin; D011374:Progesterone