A microplate immunofluorescence assay for the detection of monoclonal antibodies against viral antigens in cell culture.
A microplate immunofluorescence assay for the screening of culture fluids from large numbers of hybridoma clones has been developed. This method enables the simultaneous screening of a small volume of culture fluid from initial hybridoma clones on several different viruses or several strains of the same virus, enabling the early isolation and stabilization of the clones with the desired specificity.
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Department of Veterinary Science, University of Nebraska, Lincoln 68583.
Srikumaran
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Kelling
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000276:immunology*; D002417:Cattle; D002478:Cells, Cultured; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology; D005455:Fluorescent Antibody Technique; D007242:Herpesvirus 1, Bovine / Q000276:immunology; D008832:Microchemistry
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral