Selective aspects of mitochondrial protein turnover.
To establish the interrelationship between protein synthesis and degradation, we have developed a simple procedure. We have chosen the mitochondrial proteins, because most of them are synthesized outside mitochondria and are then imported into the organelle. This fact allows to separate easily the synthesis steps from the general turnover. By using this system we have some evidences suggesting that the in vitro protein concentration in mitochondria may be regulated by the entry of mitochondrial protein precursors. Also, we are studying the factors that regulate the import of mitochondrial protein precursors into the organelle, because this step may be essential in the regulation of the turnover of mitochondrial proteins.
Miralles
V J
VJ
Instituto de Investigaciones Citológicas, Centro Asociado del C.S.I.C., Valencia, Spain.
González-Bosch
C
C
Marcote
M J
MJ
Hernández-Yago
J
J
D000818:Animals; D001059:Apoproteins / Q000378:metabolism; D001692:Biological Transport; D003574:Cytochrome c Group / Q000378:metabolism; D045304:Cytochromes c; D008928:Mitochondria / Q000378:metabolism*; D009954:Ornithine Carbamoyltransferase / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D013095:Spermidine / Q000494:pharmacology; D013096:Spermine / Q000494:pharmacology
D001059:Apoproteins; D003574:Cytochrome c Group; D011506:Proteins; D013096:Spermine; D045304:Cytochromes c; D009954:Ornithine Carbamoyltransferase; D013095:Spermidine