Effects of L-1-methyl-histidine and the muscle dipeptides carnosine and anserine on the activities of muscle calpains.
1. Carnosine, anserine and L-1-methyl-histidine activated muscle calpain II assayed at 2.5 mM Ca2+. 2. At 5 microM Ca2+, none of these compounds activated calpain II sufficiently to bring its activity up to the level measured at 2.5 mM Ca2+. 3. Carnosine increased, whereas both anserine and L-1-methyl-histidine decreased the inhibitory effect of calpastatin on calpain II. 4. These results suggest that although the compounds are not potent activators of calpain II, the ratio of the dipeptides in muscle may have an effect on calpain II-calpastatin interaction.
Johnson
P
P
Department of Chemistry, Ohio University, Athens 45701.
Hammer
J L
JL
D000409:Alanine / Q000494:pharmacology; D000818:Animals; D000861:Anserine / Q000494:pharmacology*; D002135:Calcium-Binding Proteins / Q000494:pharmacology; D002154:Calpain / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002336:Carnosine / Q000494:pharmacology*; D002645:Chickens; D004151:Dipeptides / Q000494:pharmacology*; D006639:Histidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D008297:Male; D008762:Methylhistidines / Q000494:pharmacology*; D009130:Muscle, Smooth / Q000187:drug effects / Q000201:enzymology; D009132:Muscles / Q000187:drug effects / Q000201:enzymology*
D002135:Calcium-Binding Proteins; D004151:Dipeptides; D008762:Methylhistidines; D006639:Histidine; C033668:calpastatin; D002336:Carnosine; D002154:Calpain; D000861:Anserine; D000409:Alanine