Explants of human oral epithelium exposed to viruses and cancer chemotherapeutics.
Cultures of proliferating epithelial cells were established from explants of normal human oral epithelium from healthy young volunteers. The epithelial cells were found permissive for herpes simplex virus type 1 and type 2, coxsackie virus A-4 and A-16, adenovirus type 5, measles vaccine, rubella and influenza type A virus-. Medium from DEAE-pretreated epithelial cultures infected with two subtypes of human immunodeficiency virus-1 showed an increasing content of virusprotein with time by antigen ELISA testing. In contrast there was no evidence of infection with coxsackie virus type B-2, cytomegalovirus, Epstein-Barr virus and varicella zoster virus. Treatment of the epithelial cells with a non-cytotoxic dose of cancer chemotherapeutic prior to or after infection with coxsackie virus A-4 or herpes simplex virus type 1 influenced the virus production dependent on both compound, mode of application, and virus. Adriamycin (doxorubicin) in low dose was found to stimulate the production of the two viruses.
Ebbesen
P
P
Danish Cancer Society, Department of Virus and Cancer, Aarhus.
Petersen
P M
PM
Jepsen
A
A
Nørskov-Lauritsen
N
N
Nielsen
C M
CM
Philipsen
H P
HP
Arenholt-Bindslev
D
D
Nara
P
P
D000328:Adult; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002166:Camptothecin / Q000494:pharmacology; D002478:Cells, Cultured; D004267:DNA Viruses / Q000187:drug effects / Q000502:physiology*; D004317:Doxorubicin / Q000494:pharmacology; D004770:Enterovirus / Q000187:drug effects / Q000502:physiology; D004848:Epithelium / Q000187:drug effects / Q000382:microbiology; D015497:HIV-1 / Q000187:drug effects / Q000502:physiology; D006801:Humans; D009061:Mouth Mucosa / Q000187:drug effects / Q000382:microbiology*; D012328:RNA Viruses / Q000187:drug effects / Q000502:physiology*; D018139:Simplexvirus / Q000187:drug effects / Q000502:physiology; D014750:Vincristine / Q000494:pharmacology; D014777:Virus Diseases
D000970:Antineoplastic Agents; D014750:Vincristine; D004317:Doxorubicin; D002166:Camptothecin