title: Characterization of four herbicide-resistant mutants of Rhodopseudomonas viridis by genetic analysis, electron paramagnetic resonance, and optical spectroscopy.
abstract: Herbicides of the triazine class block electron transfer in the photosynthetic reaction centers of purple bacteria and PSII of higher plants. They are thought to act by competing with one of the electron acceptors, the secondary quinone, QB, for its binding site. Several mutants of the purple bacterium Rhodopseudomonas viridis resistant to terbutryn [2-(methylthio)-4-(ethylamino)-6-(tert-butylamino)-s-triazine] have been isolated by their ability to grow photosynthetically in the presence of the herbicide. Sequence analysis of the genes coding for the L and M subunits of the reaction center showed that four different mutants were obtained, two of them being double mutated: T1 (SerL223----Ala and ArgL217----His), T3 (PheL216----Ser and ValM263----Phe), T4 (TyrL222----Phe), and T6 (PheL216----Ser). The residues L223 and L216 are involved in binding of QB, whereas L217 and L222 are not. M263 is part of the binding pocket of the primary quinone, QA. The affinity of the reaction centers for terbutryn and the electron transfer inhibitor o-phenanthroline, determined via the biphasic charge recombination after one flash, is decreased for all mutants. The affinity for ubiquinone 9 is also decreased, except in T1. Characterization by EPR spectroscopy showed that the QB.-Fe2+ signal of T4, having a g = 1.93 peak, is different from the signals obtained with the wild type and the other mutants but very similar to those of Rhodospirillum rubrum and PSII. The results obtained by the combination of these different techniques are discussed with respect to the three-dimensional structure of the wild type and the mode of binding of ubiquinone, terbutryn, and o-phenanthroline as determined by X-ray structure analysis.
authors.affiliation: Max-Planck-Institut für Biophysik, Frankfurt, West Germany.
authors.forename: I
authors.initials: I
authors.lastname: Sinning
authors.forename: H
authors.initials: H
authors.lastname: Michel
authors.forename: P
authors.initials: P
authors.lastname: Mathis
authors.forename: A W
authors.initials: AW
authors.lastname: Rutherford
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D004352:Drug Resistance, Microbial / Q000235:genetics; D004578:Electron Spin Resonance Spectroscopy; D005796:Genes; D005798:Genes, Bacterial; D006540:Herbicides / Q000494:pharmacology*; D046911:Macromolecular Substances; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D045322:Photosynthetic Reaction Center Complex Proteins; D011487:Protein Conformation; D012241:Rhodopseudomonas / Q000187:drug effects / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014227:Triazines
chemical_list: D001426:Bacterial Proteins; D006540:Herbicides; D046911:Macromolecular Substances; D045322:Photosynthetic Reaction Center Complex Proteins; D014227:Triazines