title: A hot-spot for transposition of various Ty elements on chromosome V in Saccharomyces cerevisiae.
abstract: Ty4 is a novel transposable element in the yeast, Saccharomyces cerevisiae, which is present in only a few copies in the genome (Stucka et al. 1989). In strain C836 one of the three copies (Ty4-90) is contained in cosmid clone c90, where it resides on chromosome V. Analysis of this region reveals a "hot-spot" of transposition: in addition to Ty4-90, the locus contains a complete Ty3 element and seven singular delta, sigma and tau elements. Three tRNA genes (for His, Lys, and Ile) are located in this region, and these are closely associated with one or the other of the elements, a phenomenon commonly observed in yeast. A comparison of c90 with corresponding regions from other strains shows that the locus is highly polymorphic and that this polymorphism is explicitly associated with Ty transposition and recombination events.
authors.affiliation: Institut für Physiologische Chemie, Universität München, Federal Republic of Germany.
authors.forename: H
authors.initials: H
authors.lastname: Lochmüller
authors.forename: R
authors.initials: R
authors.lastname: Stucka
authors.forename: H
authors.initials: H
authors.lastname: Feldmann
mesh_terms: D001483:Base Sequence; D015139:Blotting, Southern; D003360:Cosmids; D004251:DNA Transposable Elements; D004271:DNA, Fungal / Q000235:genetics*; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D012343:RNA, Transfer / Q000235:genetics*; D012354:RNA, Transfer, His / Q000235:genetics; D012355:RNA, Transfer, Ile / Q000235:genetics; D012357:RNA, Transfer, Lys / Q000235:genetics; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D004271:DNA, Fungal; D012354:RNA, Transfer, His; D012355:RNA, Transfer, Ile; D012357:RNA, Transfer, Lys; D012343:RNA, Transfer