title: Characterization of the local and systemic B cell response of normal and athymic nude mice with Sindbis virus encephalitis.
abstract: During Sindbis virus (SV) encephalitis in mice B cells are an important component of the mononuclear inflammatory response and recovery depends primarily on the development of antiviral antibody. To begin to characterize various parameters of the local B cell response during SV encephalitis we have defined B cell isotype expression in brain sections, splenocytes and peripheral blood mononuclear cells (PBMC) in normal and athymic nude mice using an immunoperoxidase technique. Early (days 3-5) in SV encephalitis brain perivascular B cells are IgM or IgM/IgD-bearing lymphocytes, later (days 10-14) most B cells express one of the IgG isotypes or IgA. The pattern of isotype expression seen in the brain during the course of the encephalitis is reflected in the spleen and blood. The data suggest that progressive isotype switching results in an increasingly higher percentage of certain isotypes, especially IgG2a. Isotype switching of most B cells may occur outside of the brain, or may arise in situ from the IgM/IgD-bearing B cells found in the brain throughout the course of encephalitis. In athymic nude mice numbers of B cells in brain were markedly decreased and the cells present were primarily IgM-bearing, although IgG isotypes and IgA did appear late (day 14). The data suggest that T cells are required for recruitment of B cells into the inflammatory response as well as for normal isotype switching and peripheral B cell maturation during SV encephalitis.
authors.affiliation: Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
authors.forename: W R
authors.initials: WR
authors.lastname: Tyor
authors.forename: T R
authors.initials: TR
authors.lastname: Moench
authors.forename: D E
authors.initials: DE
authors.lastname: Griffin
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D001774:Blood Chemical Analysis; D001921:Brain / Q000166:cytology / Q000276:immunology; D002490:Central Nervous System / Q000276:immunology; D004671:Encephalitis, Arbovirus / Q000276:immunology*; D005260:Female; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D012845:Sindbis Virus; D013154:Spleen / Q000166:cytology / Q000276:immunology; D014036:Togaviridae Infections / Q000276:immunology*
chemical_list: D007070:Immunoglobulin A; D007074:Immunoglobulin G