Discordant results obtained on testing sera from immunocompromised patients for cytomegalovirus IgG by enzyme-linked immunosorbent assay and radioimmunoassay.
The investigation of sera from immunocompromised patients for antibody to CMV by ELISA, RIA, immunofluorescence (IF) and complement-fixation (CF) revealed discrepancies that reflected differences in test specificity rather than sensitivity and suggested that for the long-term serological follow-up of such patients it would be advisable not to rely on only a single assay procedure.
Booth
J C
JC
Department of Medical Microbiology, St. George's Hospital Medical School, University of London, U.K.
Kangro
H O
HO
Liu
K M
KM
el Mohandes
L
L
Tryhorn
Y S
YS
D000328:Adult; D016026:Bone Marrow Transplantation / Q000009:adverse effects; D002648:Child; D003168:Complement Fixation Tests; D003587:Cytomegalovirus / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000209:etiology / Q000517:prevention & control; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D005500:Follow-Up Studies; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000032:analysis*; D007153:Immunologic Deficiency Syndromes / Q000150:complications*; D008297:Male; D011237:Predictive Value of Tests; D011863:Radioimmunoassay
D007074:Immunoglobulin G