Bluetongue virus-17 fusion protein ns1 expressed in Escherichia coli by pUC vectors.
The cDNA coding for the major nonstructural protein, NS1, of bluetongue serotype 17 (BTV-17) was cloned previously. Using pUC plasmids, we have successfully expressed the NS1 protein in Escherichia coli as a LacZ-NS1 fusion protein. The recombinant NS1 protein reacted with rabbit anti-BTV-17 antiserum, and was thus immunologically indistinguishable from the native BTV-17 NS1 protein. This was the first bluetongue viral protein to be produced in a bacterial system.
Wang
L F
LF
Department of Biochemistry & Biophysics, University of California, Davis 95616.
Doi
R H
RH
Chuang
L F
LF
Osburn
B I
BI
Maisonnave
J
J
Benjamini
E
E
Chuang
R Y
RY
D015153:Blotting, Western; D001820:Bluetongue virus / Q000235:genetics*; D002213:Capsid / Q000096:biosynthesis / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D010957:Plasmids; D011994:Recombinant Proteins; D012087:Reoviridae / Q000235:genetics*; D014758:Viral Core Proteins / Q000096:biosynthesis / Q000235:genetics*; D014760:Viral Fusion Proteins / Q000096:biosynthesis / Q000235:genetics*; D017361:Viral Nonstructural Proteins
D004274:DNA, Recombinant; D011994:Recombinant Proteins; D014758:Viral Core Proteins; D014760:Viral Fusion Proteins; D017361:Viral Nonstructural Proteins; D004262:DNA Restriction Enzymes