Phosphoinositide metabolism and oncogenes.
The hydrolysis of phosphatidylinositol 4,5-bisphosphate plays important roles in growth factor- or oncogene-induced cell proliferation. However, it is still unknown whether the hydrolysis of phosphatidylinositol 4,5-bisphosphate is an essential step for cell growth. To solve this problem, we developed a monoclonal antibody against the lipid. Injection of the antibody into ras-transformed cells caused reversible and dose-dependent decrease in DNA synthesis and reverted the cell morphology to that of the untransformed cells. The antibody also inhibited the proliferation of erbB- and src-transformed cells but not the proliferation of untransformed or myc-transformed cells.
Takenawa
T
T
Department of Molecular Pharmacology, Tokyo Metropolitan Institute of Gerontology, Japan.
Fukami
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology*; D000918:Antibody Specificity; D002455:Cell Division / Q000187:drug effects; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000187:drug effects* / Q000378:metabolism; D002642:Chick Embryo; D004273:DNA, Neoplasm / Q000096:biosynthesis / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D009857:Oncogenes / Q000502:physiology*; D010641:Phenotype; D019269:Phosphatidylinositol 4,5-Diphosphate; D010716:Phosphatidylinositols / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D004273:DNA, Neoplasm; D019269:Phosphatidylinositol 4,5-Diphosphate; D010716:Phosphatidylinositols