title: Chemoattractant activity of Entamoeba histolytica for human polymorphonuclear neutrophils.
abstract: The ability of axenic Entamoeba histolytica trophozoites and amoebic protein preparations to stimulate chemotaxis of human polymorphonuclear neutrophils (PMN) was evaluated. Virulent E. histolytica (strain HM1:IMSS) stimulated chemotaxis (delta distance = 0.55 +/- 0.02 mm, P less than 0.01 vs. control medium). Sonicated (100 micrograms protein/ml) or homogenized (500 micrograms protein/ml) virulent amoebae also significantly stimulated PMN chemotaxis, whereas preparations of the nonpathogenic "Entamoeba-like" Laredo strain did not stimulate chemotaxis. Preparations of subcellular fractions of E. histolytica demonstrated maximal stimulation of PMN chemotaxis existed in nonvesiculated membranes and the supernatant from plasma membranes.
authors.affiliation: Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106.
authors.forename: R A
authors.initials: RA
authors.lastname: Salata
authors.forename: P
authors.initials: P
authors.lastname: Ahmed
authors.forename: J I
authors.initials: JI
authors.lastname: Ravdin
mesh_terms: D000818:Animals; D002465:Cell Movement; D002634:Chemotaxis, Leukocyte; D004748:Entamoeba histolytica / Q000472:pathogenicity; D006801:Humans; D066298:In Vitro Techniques; D009504:Neutrophils / Q000502:physiology*; D013347:Subcellular Fractions; D014774:Virulence