Activation of peripheral blood T cells via the p75 interleukin 2 receptor.
By using mAb and flow cytometry, a constitutive expression of the p75 IL-2R was revealed in human peripheral blood CD8+ T cells and TCR delta-1+ T cells as well as in CD16+ NK cells. Anti-p75 IL-2R mAb almost completely inhibited the induction of cytolytic activity in these T cells by brief exposure to IL-2, as estimated by anti-TCR/CD3 mAb-targeted cytotoxicity. While anti-p55 IL-2R mAb alone inhibited the response only modestly, maximal inhibition was achieved by combining both anti-p55 and anti-p75 IL-2R mAbs. These results indicate that the p75 IL-2R constitutively expressed on peripheral blood CD8+ T cells and TCR delta-1+ T cells is predominantly responsible for the direct activation of these cells by IL-2.
Yagita
H
H
Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan.
Nakata
M
M
Azuma
A
A
Nitta
T
T
Takeshita
T
T
Sugamura
K
K
Okumura
K
K
D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology; D003602:Cytotoxicity, Immunologic; D006801:Humans; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation; D008970:Molecular Weight; D015375:Receptors, Interleukin-2 / Q000502:physiology* / Q000648:ultrastructure; D013601:T-Lymphocytes / Q000145:classification / Q000502:physiology*
D000911:Antibodies, Monoclonal; D007376:Interleukin-2; D015375:Receptors, Interleukin-2