Mammalian tissue culture growth, viral replication, and cultivation using serum replacement factor.
Variability, cost, and availability of fetal bovine serum are in question; thus, we examined whether using a serum replacement factor in multiple mammalian tissue cultures could support not only cell growth, but also viral replication, expression, and retention of phenotypic markers. By using a serum replacement in defined media, we demonstrated multipassage cell growth of several different cell lines and viral cultivation and replication equivalent to fetal bovine serum. Moreover, by supplementing media with a serum replacement factor we observed at least a 28% financial savings.
Candal
F J
FJ
Office of Scientific Services, Centers for Disease Control, Atlanta, Georgia 30333.
Marchetti
G
G
Davis
M
M
George
V
V
Ades
E W
EW
D000818:Animals; D001769:Blood; D002352:Carrier Proteins; D002455:Cell Division; D002460:Cell Line; D003365:Costs and Cost Analysis; D003587:Cytomegalovirus / Q000254:growth & development; D003588:Cytopathogenic Effect, Viral; D006133:Growth Substances; D006801:Humans; D014776:Virus Cultivation / Q000379:methods*; D014779:Virus Replication
D002352:Carrier Proteins; D006133:Growth Substances