[Molecular weight distribution of polymeric DNA-products, formed upon the action of matrix enzymes].
Models of processive and distributive DNA synthesis and degradation catalysed by matrix enzymes were investigated. Distribution of polymer products dependent on the reaction model chosen, on the type of the matrix and on the enzyme-matrix initial concentration ratio were determined by methods of numerical modeling. Conditions were found where the scattering of the reaction polymer products was minimal. The homopolymer matrix choice of experimental condition may generate a distribution of product that obeys the Poisson distribution. Numerical investigations of polymerization (hydrolysis) processes showed that for a number of heteropolymer matrixes a distribution may exist with scattering much less than that for homopolymer matrixes. Conditions are found when processive and distributive models give different distributions of the reaction product.
Iashina
L N
LN
Likhoshvaĭ
V A
VA
Malygin
E G
EG
D001483:Base Sequence; D002384:Catalysis; D004247:DNA / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D005092:Exonucleases / Q000378:metabolism*; D006868:Hydrolysis; D008433:Mathematics; D008970:Molecular Weight; D011108:Polymers
D011108:Polymers; D004247:DNA; D004259:DNA-Directed DNA Polymerase; D005092:Exonucleases
Molekuliarno-vesovoe raspredelenie polimernykh DNK-produktov, obrazuiushchikhsia pri deĭstvii matrichnykh fermentov.