Pharmacological and functional heterogeneity of astrocytes: regional differences in phospholipase C stimulation by neuromediators.
Carbamylcholine stimulated phospholipase C activity in astrocytes in primary culture from the mesencephalon but not from the striatum or cerebral cortex of the mouse embryo. An alpha 1-adrenergic-mediated response was observed in all astrocyte populations. 2-Chloroadenosine potentiated the alpha 1-adrenergic response in mesencephalic and striatal astrocytes but not in cortical astrocytes. It also stimulated the carbamylcholine-evoked response in mesencephalic astrocytes. Through cell-cell cooperation, 2-chloroadenosine potentiated the neuronal carbamylcholine-evoked activation of phospholipase C in homotopic cocultures (neuro-glial) from the striatum but not in homotopic cocultures (neuro-glial) from the cerebral cortex or in heterotopic cocultures (cortical astrocytes-striatal neurons; striatal astrocytes-cortical neurons.
el-Etr
M
M
Chaire de Neuropharmacologie, INSERM U.114, Collège de France, Paris.
Cordier
J
J
Torrens
Y
Y
Glowinski
J
J
Prémont
J
J
D015762:2-Chloroadenosine; D000241:Adenosine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000201:enzymology*; D001921:Brain / Q000166:cytology*; D002217:Carbachol / Q000494:pharmacology*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D003342:Corpus Striatum / Q000166:cytology; D004357:Drug Synergism; D004622:Embryo, Mammalian; D008636:Mesencephalon / Q000166:cytology; D051379:Mice; D018377:Neurotransmitter Agents / Q000494:pharmacology*; D009638:Norepinephrine / Q000494:pharmacology; D010738:Type C Phospholipases / Q000378:metabolism*
D018377:Neurotransmitter Agents; D015762:2-Chloroadenosine; D002217:Carbachol; D010738:Type C Phospholipases; D000241:Adenosine; D009638:Norepinephrine