Separation of hepatocyte plasma membrane domains by free flow electrophoresis.
We have applied free flow electrophoresis to separate the canalicular and basolateral (sinusoidal and lateral) domains of rat hepatocyte plasma membranes. Hepatocyte plasma membranes were prepurified by rat zonal and discontinous sucrose gradient centrifugation. In electrophoretic separation, the canalicular membranes were more deflected toward the anode than the basolateral membranes. Na+-dependent taurocholate uptake could be measured in both membrane fractions, transport activity being highest in fractions containing the highest specific activity in the basolateral marker enzyme Na+-K+-ATPase. Thus, differences in electrophoretic mobility permit the separation of functional intact plasma membrane vesicles derived from basolateral and canalicular plasma membrane domains of rat hepatocyte.
Evers
C
C
Department of Physiology, University of Zurich, Switzerland.
Meier
P J
PJ
Biber
J
J
Murer
H
H
D000042:Absorption; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002498:Centrifugation; D004586:Electrophoresis / Q000379:methods; D066298:In Vitro Techniques; D007931:Leucyl Aminopeptidase / Q000378:metabolism; D008099:Liver / Q000166:cytology*; D008871:Microvilli / Q000032:analysis; D051381:Rats; D012964:Sodium / Q000493:pharmacokinetics; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013656:Taurocholic Acid / Q000493:pharmacokinetics
D013656:Taurocholic Acid; D012964:Sodium; D007931:Leucyl Aminopeptidase; D000254:Sodium-Potassium-Exchanging ATPase