Human nuclear protein interacting with a conservative sequence motif of Alu-family DNA repeats.
Human retrotransposons, Alu-family DNA repeats (AFRs), have variable nucleotide sequence but conservative short elements, which may have important functions, are also present. In our previous reports we have described human nuclear DNA-binding protein interacting with AFRs and evidence was presented that the protein recognizes sequence motif 5'-GGAGGC-3' which is conserved in the spacer of RNA polymerase III promoter of AFRs and in the SV40 T-antigen-dependent replication origin of AFRs. In this study it was found that double-stranded synthetic oligonucleotides containing indicated conservative sequences of AFRs actually have high-affinity binding site for HeLa nuclear protein. The data suggest that non-infected human cells contain nuclear DNA-binding protein which recognizes the conservative sequence motif of AFRs - GGAGGC.
Tomilin
N V
NV
Institute of Cytology, Academy of Sciences of the USSR, Leningrad.
Bozhkov
V M
VM
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002417:Cattle; D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D006367:HeLa Cells / Q000032:analysis; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D009841:Oligonucleotides / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012320:RNA Polymerase III / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid
D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D009841:Oligonucleotides; D012321:DNA-Directed RNA Polymerases; D012320:RNA Polymerase III