A human endonuclease incises ultraviolet-irradiated DNA at cytosines and oxidized DNA at thymines.
Both ultraviolet irradiation and oxidation of DNA produce a variety of pyrimidine base damages. A human endonuclease recognizes such altered bases on these DNA substrates. This human endonuclease incises ultraviolet-irradiated DNA exclusively at sites of photochemically modified cytosines. The precise sites of incision by the human enzyme were determined by DNA sequencing. Chemically oxidized DNA was incised exclusively at thymine loci. The degree of enzymic cleavage at cytosine photoproducts was identical at each site. However, the extent of incision at selected oxidized thymine residues varied within the DNA sequence. These results indicate that the distribution of thymine oxidative modifications is influenced by the neighboring DNA bases.
Gallagher
P E
PE
Department of Pathology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.
Weiss
R B
RB
Brent
T P
TP
Duker
N J
NJ
D001483:Base Sequence; D003596:Cytosine / Q000378:metabolism*; D004247:DNA / Q000378:metabolism* / Q000528:radiation effects; D004260:DNA Repair; D003720:Densitometry; D004720:Endonucleases / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D013941:Thymine / Q000378:metabolism*; D014466:Ultraviolet Rays
D003596:Cytosine; D004247:DNA; D004720:Endonucleases; D013941:Thymine