Aflatoxin B1 formamidopyrimidine adducts in human hepatocarcinogenesis: a preliminary report.
The presence of aflatoxin-B1-formamidopyrimidine, a persistent aflatoxin-deoxyribonucleic acid (DNA) adduct, was investigated in vivo by immunohistochemical analysis in 14 paired hepatocellular carcinoma and nontumorous human liver tissue sections using a monoclonal anti-aflatoxin-B1-formamidopyrimidine antibody. Nuclear and cytoplasmic accumulations of adducts were found in 4 of 14 nontumorous specimens but in none of 14 tumorous tissues and in none of three normal control livers. In vitro adduct formation and cellular DNA was investigated with a modified DNA immunoblot assay. These studies revealed (a) no significant difference in the amount of adduct formed by DNA samples with or without integrated hepatitis B virus DNA, (b) no difference in the amount of adduct formed with DNA from either tumorous or nontumorous tissues from a given individual, and (c) remarkable and reproducible differences in the capacity of DNA from different individuals to form in vitro adducts. Our DNA immunoblot assay will facilitate further studies on the molecular role of aflatoxin-B1-form-amidopyrimidine adducts in human hepatocarcinogenesis.
Lee
H S
HS
Department of Laboratory Medicine, University of California, San Francisco.
Sarosi
I
I
Vyas
G N
GN
D016604:Aflatoxin B1; D000348:Aflatoxins / Q000032:analysis*; D000911:Antibodies, Monoclonal; D002273:Carcinogens / Q000032:analysis*; D006528:Carcinoma, Hepatocellular / Q000032:analysis* / Q000139:chemically induced; D004247:DNA / Q000032:analysis; D004273:DNA, Neoplasm / Q000032:analysis; D006801:Humans; D015151:Immunoblotting; D008113:Liver Neoplasms / Q000032:analysis* / Q000139:chemically induced; D011743:Pyrimidines / Q000032:analysis*
D000348:Aflatoxins; D000911:Antibodies, Monoclonal; D002273:Carcinogens; D004273:DNA, Neoplasm; D011743:Pyrimidines; C061392:aflatoxin B1-formamidopyrimidine; D004247:DNA; D016604:Aflatoxin B1