Identification of H- and L-ferritin subunit binding sites on human T and B lymphoid cells.
Monoclonal antibodies to the H and L subunits of ferritin were used in indirect immunofluorescent studies to investigate the subunit specificity of ferritin binding to transformed T and B lymphoid cell lines. Pre-incubation of cells with recombinant H ferritin followed by antibodies to the H subunit produced a labelling pattern in which all cells showed strong fluorescent stippling. Corresponding experiments with liver ferritin and an antibody to the L subunit revealed that most cells were lightly stippled, but 3-5% of the cells were intensely labelled. These two labelling patterns were distinct as assessed by cross-blocking experiments with recombinant H and liver ferritins. These results imply that different binding sites exist for each subunit.
Anderson
G J
GJ
Department of Medicine, University of Queensland, Brisbane, Australia.
Faulk
W P
WP
Arosio
P
P
Moss
D
D
Powell
L W
LW
Halliday
J W
JW
D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000378:metabolism*; D001665:Binding Sites; D002051:Burkitt Lymphoma / Q000378:metabolism; D002460:Cell Line; D005293:Ferritins / Q000276:immunology / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D033862:Iron-Binding Proteins; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000378:metabolism
D000911:Antibodies, Monoclonal; D033862:Iron-Binding Proteins; D011956:Receptors, Cell Surface; C027293:ferritin receptor; D005293:Ferritins