Nucleotide sequence of coronavirus TGEV genomic RNA: evidence for 3 mRNA species between the peplomer and matrix protein genes.
The region of the TGEV genome between the E1-matrix protein gene and the E2-peplomer protein gene has been sequenced from a cDNA clone. The consensus recognition sequence, 5'TTAA CTAAAC was found upstream from 3 large open reading frames. In coronaviruses these homologous recognition sequences are involved in the initiation of transcription suggesting that there are 3 mRNA species in this region of the TGEV genome. Northern blot analysis and nuclease S1 mapping confirmed the presence of 3 mRNA species between mRNA 3 encoding the E2-peplomer protein and mRNA 6 encoding the E1-matrix protein. The 5' regions of these 3 mRNAs encode potential polypeptides of predicted molecular weight; 7859, 27744 and 9287, respectively. The potential translation product of ORF B (27744 Da) is considerably larger than previously reported and could be difficult to distinguish by size from the E1-matrix protein.
Wesley
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RD
USDA, Agricultural Research Service, National Animal Disease Center, Ames Iowa 50010.
Cheung
A K
AK
Michael
D D
DD
Woods
R D
RD
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D003332:Coronaviridae / Q000235:genetics*; D004247:DNA / Q000032:analysis; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis*; D013552:Swine; D005760:Transmissible gastroenteritis virus / Q000235:genetics*; D014763:Viral Matrix Proteins / Q000032:analysis / Q000235:genetics*
D012333:RNA, Messenger; D012367:RNA, Viral; D014763:Viral Matrix Proteins; D004247:DNA