title: Acute myeloid leukemia, FAB M-1 microgranular variant: a multiparameter study.
abstract: Acute leukemia was diagnosed in 62 adults and children over a recent 13-month period. Using light microscopy, cytochemical profiles, surface markers, and cytogenetics, 25 cases were classified as acute myeloid leukemia (AML) and 32 as acute lymphoblastic leukemia (ALL). The remaining 5 cases of de novo acute leukemia were unclassifiable. The routine cytochemical battery used on these 62 cases included: myeloperoxidase, sudan black B, nonspecific esterase, and periodic acid-Schiff (PAS). Flow markers utilized were: T3, T4, T5, T8, T10, T11, B1, B4, kappa, lambda, Ia, CALLA, Mo1, Mo2, My4, My7, My8, and My9. TdT was performed by immunoperoxidase and ELISA methods. The five unclassified cases were cytochemically negative and expressed no B- or T-cell-specific antigens, or TdT positivity. The morphologic differential diagnosis was between FAB L-2 and M-1. Karyotypic abnormalities involving chromosomes 3 and 7 were suggestive of myeloid origin in 2 of 4 patients studied. Flow cytometry demonstrated My7 on greater than 50% of blasts from two cases. Myeloperoxidase ultracytochemistry showed reaction product in small primary granules of blasts from all 5 cases. Positive cells contained only 1-2 granules/cell profile. The number of positive cells per case was in the range 10-20%. We conclude from this study that ultracytochemistry is very useful in providing definitive diagnosis and accurate subclassification of some AML FAB M-1 cases, particularly when light microscopic cytochemistry, cytogenetics, and flow cytometric markers are noncontributory. We propose to designate these acute "unclassified" leukemias as AML FAB M-1 "microgranular" type.
authors.affiliation: Department of Pathology Clinical Hematology, Los Angeles County - USC Medical Center.
authors.forename: D
authors.initials: D
authors.lastname: Nguyen
authors.forename: R K
authors.initials: RK
authors.lastname: Brynes
authors.forename: L
authors.initials: L
authors.lastname: Macaulay
authors.forename: B
authors.initials: B
authors.lastname: Kaplan
authors.forename: L C
authors.initials: LC
authors.lastname: Pinter-Brown
mesh_terms: D000208:Acute Disease; D000328:Adult; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D001853:Bone Marrow / Q000648:ultrastructure; D003594:Cytoplasmic Granules / Q000201:enzymology; D003937:Diagnosis, Differential; D005260:Female; D005434:Flow Cytometry; D006403:Hematologic Tests; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D007621:Karyotyping; D007938:Leukemia / Q000473:pathology; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009195:Peroxidase / Q000032:analysis; D010641:Phenotype
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D009195:Peroxidase