title: cDNA sequence for the alpha M subunit of the human neutrophil adherence receptor indicates homology to integrin alpha subunits.
abstract: The receptor on human neutrophils (polymorphonuclear leukocytes) that mediates cellular adherence consists of two noncovalently associated subunits, designated alpha M (Mac-1 alpha, Mol alpha, or CD11b; Mr, 170,000) and beta (Mac-1 beta, Mol beta, or CD18; Mr, 100,000). We isolated a cDNA clone for the human neutrophil alpha M subunit by screening a lambda gt 11 cDNA library made from chronic myelogenous leukemia neutrophils by using an affinity-purified rabbit polyclonal antibody directed against the alpha M subunit. We used this cDNA clone to obtain additional clones from cDNA libraries made from differentiated HL-60 promyelocytic leukemia cells. Together these cDNAs constitute the complete 1137-amino acid sequence for the mature human alpha M subunit protein. The deduced amino acid sequence indicates the presence of an extensive extracellular domain with three putative metal-binding regions, (i) an amino acid region that is homologous to the A domain of von Willebrand factor, (ii) a 26-amino acid hydrophobic sequence that is a potential transmembrane domain, and (iii) a 19-amino acid cytoplasmic region. The amino acid sequence for the human neutrophil alpha M subunit contains regions that are closely related to amino acid sequences of adhesion receptors belonging to the integrin family.
authors.affiliation: Medical Research Division, Seattle Veterans Administration Medical Center, WA 98108.
authors.forename: D D
authors.initials: DD
authors.lastname: Hickstein
authors.forename: M J
authors.initials: MJ
authors.lastname: Hickey
authors.forename: J
authors.initials: J
authors.lastname: Ozols
authors.forename: D M
authors.initials: DM
authors.lastname: Baker
authors.forename: A L
authors.initials: AL
authors.lastname: Back
authors.forename: G J
authors.initials: GJ
authors.lastname: Roth
mesh_terms: D000595:Amino Acid Sequence; D000943:Antigens, Differentiation / Q000235:genetics*; D001483:Base Sequence; D001798:Blood Proteins / Q000235:genetics*; D015152:Blotting, Northern; D018821:CD18 Antigens; D003062:Codon / Q000235:genetics; D004247:DNA / Q000235:genetics*; D005796:Genes; D006801:Humans; D046911:Macromolecular Substances; D016177:Macrophage-1 Antigen; D008562:Membrane Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000276:immunology*; D011971:Receptors, Immunologic / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D000943:Antigens, Differentiation; D001798:Blood Proteins; D018821:CD18 Antigens; D003062:Codon; D046911:Macromolecular Substances; D016177:Macrophage-1 Antigen; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic; D004247:DNA