title: Transformation by activated ras or fos prevents myogenesis by inhibiting expression of MyoD1.
abstract: Transformation of myoblasts by activated ras inhibits myogenic differentiation. We demonstrate that this oncogene inhibits expression of the muscle regulatory factors MyoD1 and myogenin. Expression of retroviral-encoded MyoD1 in ras-transformed myoblasts leads to the re-expression of both terminal differentiation markers and lineage markers expressed in proliferating myoblasts (including endogenous MyoD1 and myogenin), suggesting that ras inhibits myogenic differentiation in a manner dependent on the loss of MyoD1 expression. In addition, we show that fos transformation of myoblasts inhibits muscle differentiation by a similar mechanism.
authors.affiliation: Department of Genetics, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
authors.forename: A B
authors.initials: AB
authors.lastname: Lassar
authors.forename: M J
authors.initials: MJ
authors.lastname: Thayer
authors.forename: R W
authors.initials: RW
authors.lastname: Overell
authors.forename: H
authors.initials: H
authors.lastname: Weintraub
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002454:Cell Differentiation; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000235:genetics; D005786:Gene Expression Regulation; D011905:Genes, ras; D066298:In Vitro Techniques; D051379:Mice; D009124:Muscle Proteins / Q000235:genetics; D009132:Muscles / Q000502:physiology*; D017570:MyoD Protein; D018008:Myogenin; D009687:Nuclear Proteins / Q000235:genetics*; D010750:Phosphoproteins / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000502:physiology*; D016760:Proto-Oncogene Proteins c-fos; D014170:Transformation, Genetic
chemical_list: D004268:DNA-Binding Proteins; D009124:Muscle Proteins; D017570:MyoD Protein; C057363:MyoD1 myogenic differentiation protein; C503801:Myog protein, mouse; D018008:Myogenin; D009687:Nuclear Proteins; D010750:Phosphoproteins; D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos