title: [Identification of human porins. II. Characterization and primary structure of a 31-lDa porin from human B lymphocytes (Porin 31HL)].
abstract: We characterize and describe for the first time the primary structure of a human porin with the molecular mass of 31 kDa derived from the plasmalemm of B-lymphocytes (Porin 31HL). Porin 31HL is shown to be a basic, channel forming membrane protein. The protein chain is composed of 282 amino acids with a relative molecular mass of 30641 Da without derivatisation. It is not a glycoprotein. The N-terminus is acetylated. Altogether the amino-acid sequence shows 56% hydrophilic or charged amino acids arranged in alternating regions of hydrophilic or hydrophobic character as it is typical for porins. In addition the 18 N-terminal amino acids of Porin 31HL can be arranged to an amphilic alpha-helix like in other porins. Porin 31HL shows approx. 29% or 24% identity to the primary structure of mitochondrial porins of Neurospora crassa and Saccharomyces cerevisiae. Partial data on mitochondrial porins from rat kidney and beef heart show sequence identity of about 90% to the human B cell porin elaborated here.
authors.affiliation: Max-Planck-Institut für experimentelle Medizin, Abteilung Immunchemie, Göttingen.
authors.forename: H
authors.initials: H
authors.lastname: Kayser
authors.forename: H D
authors.initials: HD
authors.lastname: Kratzin
authors.forename: F P
authors.initials: FP
authors.lastname: Thinnes
authors.forename: H
authors.initials: H
authors.lastname: Götz
authors.forename: W E
authors.initials: WE
authors.lastname: Schmidt
authors.forename: K
authors.initials: K
authors.lastname: Eckart
authors.forename: N
authors.initials: N
authors.lastname: Hilschmann
mesh_terms: D000595:Amino Acid Sequence; D001402:B-Lymphocytes / Q000032:analysis*; D001425:Bacterial Outer Membrane Proteins / Q000302:isolation & purification; D002460:Cell Line; D002462:Cell Membrane / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008928:Mitochondria / Q000032:analysis; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009492:Neurospora crassa / Q000032:analysis; D018272:Porins; D011489:Protein Denaturation; D012441:Saccharomyces cerevisiae / Q000032:analysis
chemical_list: D001425:Bacterial Outer Membrane Proteins; D018272:Porins
vernacular_title: Zur Kenntnis der Porine des Menschen. II. Charakterisierung und Primärstruktur eines 31-kDA-Porins aus menschlichen B-Lymphozyten (Porin 31HL).