title: Analysis of the chromosomal location of two copies of a Bordetella pertussis insertion sequence.
abstract: IS481v1 and IS481v2 are two copies of a Bordetella pertussis insertion sequence element. We have shown that IS481v1 is located within 3 kbp of the start of the adenylate cyclase gene whilst IS481v2 is immediately adjacent to the end of the agglutinogen 2 gene and provides the stop codon for that gene. In addition, IS481v1 and IS481v2 were present at these two specific sites in nine strains of B. pertussis, including two Phase IV strains which expressed neither adenylate cyclase nor agglutinogen 2 and three Phase I strains which did not express agglutinogen 2. The loss of expression in these strains is not the result of DNA rearrangements at the sites of IS481v1 or IS481v2.
authors.affiliation: Division of Bacteriology, National Institute for Biological Standards and Control, South Mimms, Hertfordshire, UK.
authors.forename: W L
authors.initials: WL
authors.lastname: McPheat
authors.forename: J H
authors.initials: JH
authors.lastname: Hanson
authors.forename: I
authors.initials: I
authors.lastname: Livey
authors.forename: J S
authors.initials: JS
authors.lastname: Robertson
mesh_terms: D037361:Adenylate Cyclase Toxin; D000262:Adenylyl Cyclases / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D001886:Bordetella pertussis / Q000235:genetics*; D002872:Chromosome Deletion; D004251:DNA Transposable Elements; D005786:Gene Expression Regulation; D015321:Gene Rearrangement; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D010566:Virulence Factors, Bordetella / Q000235:genetics*
chemical_list: D037361:Adenylate Cyclase Toxin; D004251:DNA Transposable Elements; D010566:Virulence Factors, Bordetella; D000262:Adenylyl Cyclases