title: Production and in vivo biologic actions of recombinant mouse interferon alpha 2.
abstract: We have expressed a recombinant mouse interferon alpha (r.Mu-IFN alpha 2) in Escherichia coli under the control of a tryptophan promoter using a synthetic adaptor formed by annealing two partially complementary oligonucleotides which introduced an ATG start codon and re-established the complete coding sequence of the mature IFN alpha 2 protein in the expression vector. Levels of up to 10(7) reference units of Mu-IFN alpha 2 per liter of culture were obtained using this construction. This recombinant mouse interferon alpha 2 exhibited antiviral activity in mice infected with EMC virus and antitumor activity in mice inoculated with Friend leukemia cells in a manner similar to that of natural mouse interferon alpha/beta.
authors.affiliation: Laboratory of Viral Oncology, Centre National de la Recherche Scientifique, Villejuif.
authors.forename: M
authors.initials: M
authors.lastname: Dron
authors.forename: M G
authors.initials: MG
authors.lastname: Tovey
authors.forename: C
authors.initials: C
authors.lastname: Maury
authors.forename: P
authors.initials: P
authors.lastname: Eid
authors.forename: F
authors.initials: F
authors.lastname: Meyer
authors.forename: I
authors.initials: I
authors.lastname: Gresser
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004686:Encephalomyocarditis virus / Q000187:drug effects / Q000502:physiology; D004926:Escherichia coli / Q000235:genetics; D005622:Friend murine leukemia virus / Q000187:drug effects / Q000502:physiology; D066298:In Vitro Techniques; D007370:Interferon Type I / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D007942:Leukemia, Experimental / Q000628:therapy; D051379:Mice; D008811:Mice, Inbred DBA; D008969:Molecular Sequence Data; D010957:Plasmids; D011994:Recombinant Proteins; D014779:Virus Replication / Q000187:drug effects
chemical_list: D007370:Interferon Type I; D011994:Recombinant Proteins; D004247:DNA