[Study on molecular hybridization with biotin-labelled HPV 16 DNA probe in human cervical carcinoma].
Biotin-labelled human papillomavirus (HPV) 16 type DNA probe was prepared by the techniques of molecular biology. And dot hybridization technique was used to detect the HPV 16 homologous sequences in the tissues DNA of human cervical carcinoma. The results indicated that 16 cases out of 28 of the human cervical carcinoma tissues were positive. The positive rate was 57%. The other 4 cases of normal uterine cervix tissues were negative. Only 1 in 4 chronic cervicitis tissues showed positive. The HPV 16 plasmid DNA, as the positive control group, showed strong positive, while lambda-phage DNA was negative. The results have shown that the genome of the HPV actually exists in the tissue of the cervical carcinoma and that there is a close relationship between the cervical carcinoma and HPV infection. This experiment adopted the Biotin-labelled HPV 16 DNA probe. And it may provide us with a quick and sensitive method for investigation of the infection of HPV and its role in the carcinogenesis of cervical carcinoma.
Li
Y
Y
Huang
G Q
GQ
Mao
T
T
Huang
Y F
YF
Xiao
H Y
HY
Liu
B L
BL
D001710:Biotin; D015139:Blotting, Southern; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D015342:DNA Probes; D005260:Female; D006801:Humans; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000235:genetics*
D015342:DNA Probes; D001710:Biotin