Structural differences between the glucocorticoid, dioxin and oxysterol receptors from rat liver cytosol.
The rat hepatic glucocorticoid, dioxin and oxysterol receptors were subjected to high performance liquid chromatography on size-exclusion and anion-exchange columns. Both the glucocorticoid receptor and the dioxin receptor had a Stokes radius Rs approximately 7.5 nm, expected value for heteromeric complexes containing a dimer of the Mr approximately 90,000 heat shock protein, hsp90 (Rs approximately 7.0 nm). The oxysterol receptor represented a much smaller entity (Rs approximately 6.0 nm). When analyzed on a Mono Q anion-exchange column, the molybdate-stabilized glucocorticoid receptor and dioxin receptor eluted as single peaks at approximately 0.30 M and 0.26-0.28 M NaCl, respectively, whereas the oxysterol receptor represented a less negatively charged species (0.11-0.14 M NaCl). Following washing of the Mono Q column with molybdate-free buffer, the activated monomeric glucocorticoid receptor was detected (0.10-0.12 M NaCl). In contrast, no modification in the elution pattern of the dioxin receptor and the oxysterol receptor was observed. These data demonstrate differences in the physico-chemical properties of the glucocorticoid, dioxin and oxysterol receptors, respectively, which might reflect structural differences.
Denis
M
M
Department of Medical Nutrition, Karolinska Institute, Huddinge University Hospital, Sweden.
Wilhelmsson
A
A
Cuthill
S
S
Poellinger
L
L
Gustafsson
J A
JA
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003600:Cytosol / Q000032:analysis; D004268:DNA-Binding Proteins / Q000502:physiology* / Q000648:ultrastructure; D004147:Dioxins; D008099:Liver / Q000032:analysis; D008970:Molecular Weight; D051381:Rats; D018336:Receptors, Aryl Hydrocarbon; D011955:Receptors, Drug / Q000378:metabolism / Q000648:ultrastructure*; D011965:Receptors, Glucocorticoid / Q000378:metabolism / Q000648:ultrastructure*; D011987:Receptors, Steroid / Q000378:metabolism / Q000648:ultrastructure*; D000098825:Oxysterol Binding Proteins
D004268:DNA-Binding Proteins; D004147:Dioxins; D018336:Receptors, Aryl Hydrocarbon; D011955:Receptors, Drug; D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; D000098825:Oxysterol Binding Proteins