Herpes simplex-1 DNA polymerase. Identification of an intrinsic 5'----3' exonuclease with ribonuclease H activity.
The herpes simplex virus-1 DNA polymerase is a heterodimer of Mr 190,000 which consists of the products of the UL30 (Pol) and UL42 genes. The 136-kilodalton Pol gene product contains an intrinsic ribonuclease H activity that specifically degrades RNA.DNA heteroduplexes or duplex DNA substrates in the 5'----3' direction. It can therefore catalyze the excision of the RNA primers that initiate the synthesis of Okazaki fragments at a replication fork during herpes DNA replication.
Crute
J J
JJ
Department of Biochemistry, Beckman Center, Stanford University, California 94305-5307.
Lehman
I R
IR
D000818:Animals; D004259:DNA-Directed DNA Polymerase / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D004722:Endoribonucleases / Q000378:metabolism*; D043211:Exodeoxyribonuclease V; D005090:Exodeoxyribonucleases / Q000378:metabolism*; D007700:Kinetics; D008970:Molecular Weight; D016914:Ribonuclease H; D018139:Simplexvirus / Q000201:enzymology* / Q000235:genetics; D013379:Substrate Specificity; D014709:Vero Cells
D004259:DNA-Directed DNA Polymerase; D004722:Endoribonucleases; D005090:Exodeoxyribonucleases; D043211:Exodeoxyribonuclease V; D016914:Ribonuclease H