title: [Synthetic DNA-bindings ligands containing reaction centers specific for AT- and GC-pairs in DNA].
abstract: In the present communication design, synthesis and DNA binding activities of three bis-netropsins and two netropsin analogs containing two N-propylpyrrolecarboxamide fragments linked covalently to peptides Gly-Gly-(analog I) and Val-Val-Val-Gly-Gly-(analog II) are reported. Each bis-netropsin consists of two netropsin-like fragments attached to peptides -Gly-Cys-Gly-NH2 (compound IIIa), H-Gly-Cys-Gly-Gly-Gly-(compound IV) or Gly-Cys-Sar-NH2 (compound IIIb) which are linked symmetrically via S-S bonds. Physico-chemical studies show that each bis-netropsin carries 6 AT-specific reaction centers and covers approximately 10 base pairs upon binding to poly(dA).poly(dT). This indicates that two netropsin-like fragments of the bis-netropsin molecule are implicated in specific interaction with DNA base pairs. The peptide fragments of bis-netropsins IIIa and IV form small beta-sheets containing two-GC-specific reaction centers. The DNase I cleavage patterns of bis-netropsin-DNA complexes visualized by high resolution gel electrophoresis show that the preferred binding sites for bis-netropsins IIIa and IV are identical and contain two runs of three or more AT pairs separated by two GC pairs. Specificity determinants of netropsin analog II binding in the beta-associated dimeric form are identical to those of bis-netropsin IIIa thereby indicating that there is a similarity in the structure of complexes formed by these ligands with DNA. In the monomeric form analog II exhibits binding specificity identical to that of analog I. Replacement of C-terminal glycine residues by sarcosines in the peptide fragments of bis-netropsin IIIa leads to a decrease in the affinity of ligand for DNA.
authors.forename: T A
authors.initials: TA
authors.lastname: Leĭnsoo
authors.forename: V A
authors.initials: VA
authors.lastname: Nikolaev
authors.forename: S L
authors.initials: SL
authors.lastname: Grokhovskiĭ
authors.forename: A N
authors.initials: AN
authors.lastname: Surovaia
authors.forename: N Iu
authors.initials: NIu
authors.lastname: Sidorova
authors.forename: S A
authors.initials: SA
authors.lastname: Strel'tsov
authors.forename: A S
authors.initials: AS
authors.lastname: Zasedatelev
authors.forename: A L
authors.initials: AL
authors.lastname: Zhuze
authors.forename: G V
authors.initials: GV
authors.lastname: Gurskiĭ
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D002942:Circular Dichroism; D004247:DNA / Q000378:metabolism*; D006146:Guanidines / Q000138:chemical synthesis*; D006868:Hydrolysis; D008024:Ligands; D008969:Molecular Sequence Data; D009429:Netropsin / Q000031:analogs & derivatives / Q000138:chemical synthesis* / Q000378:metabolism; D009690:Nucleic Acid Conformation; D011089:Polydeoxyribonucleotides / Q000138:chemical synthesis / Q000378:metabolism
chemical_list: D006146:Guanidines; D008024:Ligands; D011089:Polydeoxyribonucleotides; D009429:Netropsin; D004247:DNA
vernacular_title: Sinteticheskie DNK-sviazyvaiushchie ligandy, soderzhashchie reaktsionnye tsentry, spetsifichnye k AT- i GC-param osnovaniĭ DNK.