Effect of oxygen-derived free radicals and oxidants on the degradation in vitro of membrane phospholipids.
The abilities of chemically generated hydroxyl radical (OH.), superoxide anion (O.-) and hydrogen peroxide (H2O2) to degrade rat myocardial membrane phospholipids previously labeled with [1-14C]arachidonic acid were studied. HO. and H2O2, but not O2.-, caused the degradation of phosphatidylcholine (PC), phosphatidylethanolamine (PE), and phosphatidylinositol (PI). With OH. and H2O2, the loss of radiolabel in PC was accompanied by an increase in the radiolabel of lysophosphatidylcholine (LPC), but not in that of free fatty acid (FFA). These results suggest the hydrolysis of 1-oxygen ester bond of PC by HO. and that H2O2 and that HO. and H2O2, but not O.-, are detrimental to the structure and function of membrane phospholipids. However, since microM amounts of HO. and mM amounts of H2O2 were necessary to affect the membrane phospholipids, it is likely that in the reperfused myocardium only HO., but not H2O2, may directly cause the breakdown of membrane phospholipids.
Prasad
M R
MR
Department of Surgery, University of Connecticut School of Medicine, Farmington 06032.
Das
D K
DK
D000818:Animals; D002374:Catalase / Q000494:pharmacology; D005609:Free Radicals; D006861:Hydrogen Peroxide / Q000378:metabolism; D006878:Hydroxides / Q000378:metabolism; D017665:Hydroxyl Radical; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism*; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013482:Superoxide Dismutase / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism; D013890:Thiourea / Q000494:pharmacology / Q000031:analogs & derivatives
D005609:Free Radicals; D006878:Hydroxides; D008563:Membrane Lipids; D010743:Phospholipids; D013481:Superoxides; D017665:Hydroxyl Radical; C038983:1,3-dimethylthiourea; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase; D013890:Thiourea