Cloning of the pig aminopeptidase N gene. Identification of possible regulatory elements and the exon distribution in relation to the membrane-spanning region.
We have isolated four lambda-phages covering the complete pig aminopeptidase N/CD13 gene. The sequence of 2.85 kbp encompasses 1.18 kbp of the 5' upstream region and 1.67 kbp of the structural gene. In the promoter region we find a TATA box and potential binding sites for CTF-1/NF-1 and AP-2. By sequence comparisons we have found three domains showing similarity to promoter regions of the genes encoding human alpha 1-antitrypsin and human intestinal alkaline phosphatase. The gene sequence includes the first three exons and two introns. It shows that a single exon encodes the cytoplasmic tail, the membrane anchor and the junctional peptide.
Olsen
J
J
Department of Biochemistry C, Panum Institute, University of Copenhagen, Denmark.
Sjöström
H
H
Norén
O
O
D000469:Alkaline Phosphatase / Q000235:genetics; D000595:Amino Acid Sequence; D000626:Aminopeptidases / Q000235:genetics*; D000818:Animals; D015703:Antigens, CD / Q000235:genetics*; D015214:Antigens, Differentiation, Myelomonocytic / Q000235:genetics*; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D018826:CD13 Antigens; D002462:Cell Membrane / Q000201:enzymology*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004262:DNA Restriction Enzymes; D004720:Endonucleases; D005091:Exons; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013552:Swine; D014158:Transcription, Genetic; D000515:alpha 1-Antitrypsin / Q000235:genetics
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D012333:RNA, Messenger; D000515:alpha 1-Antitrypsin; D004247:DNA; D004720:Endonucleases; D004262:DNA Restriction Enzymes; D000469:Alkaline Phosphatase; D015719:Single-Strand Specific DNA and RNA Endonucleases; D000626:Aminopeptidases; D018826:CD13 Antigens