[SEM study on Chlamydia trachomatis in cultured McCoy cell--with special reference to surface projections and intra-inclusion network].
A study on the growth cycle of C. trachomatis of genital origin in McCoy cells has produced the following results: 1) Two kinds of surface projections, spikelike and hemispheric, were observed. The spikelike projections were recognized 50 hours after infection in larger C. trachomatis of 1.0 micron in diameter. From the surface these C. trachomatis measured 21-52 nm in height with diameters of 25-35 nm. Hemispheric projections were observed 72 hours after infection in smaller C. trachomatis of under 0.3 micron in diameter. Their average diameter was 34.0 nm. These projections were hexagonally arranged with the distances between the center of adjacent projections at 40-45 nm. 2) Intra-inclusion networks were observed. These were recognized in inclusions 72 hours after infection. Their structure was three dimensional with lineal materials extending from one C. trachomatis to another. The networks were composed of granular particles averaging 53.9 nm in diameter. These granular particles were not noticed after amylase treatment and lineal material of 33.5 nm in width was noticed instead. This lineal material may be regarded as the axis of the network. Structurally, the network is an axis 33.5 nm wide with glycogen granules attached to the perimeter. The network structures were classified into long, short, and inclusion types.
Kurabayashi
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D000328:Adult; D002478:Cells, Cultured; D002692:Chlamydia trachomatis / Q000254:growth & development / Q000648:ultrastructure*; D005260:Female; D006801:Humans; D002479:Inclusion Bodies / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning