Localization of cGMP in the cerebellum of the adult rat: an immunohistochemical study.
The localization of cGMP in the cerebellum of the adult rat after fixation with formaldehyde was studied with an antibody raised against a cGMP-formaldehyde-thyroglobulin conjugate. Three different protocols were used: (1) in vitro incubation of 300 microns cerebellar slices followed by fixation and cryostat sectioning; (2) in vitro incubation of 100 microns cerebellar slices followed by fixation with no further sectioning; (3) perfusion fixation of the anesthetized rat followed by vibratome sectioning. All 3 protocols gave essentially the same results: cGMP-immunoreactivity was found predominantly in Bergmann fibers in the molecular layer, in Bergmann cell bodies in the Purkinje cell layer (but not in Purkinje cells), and in astroglial cells in the granular layer.
de Vente
J
J
Department of Pharmacology, Faculty of Medicine, Free University, Amsterdam, The Netherlands.
Bol
J G
JG
Steinbusch
H W
HW
D000818:Animals; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism*; D006152:Cyclic GMP / Q000378:metabolism*; D007150:Immunohistochemistry; D051381:Rats; D011919:Rats, Inbred Strains
D006152:Cyclic GMP