Differential expression of the c-erbB-2 gene in human small cell and non-small cell lung cancer.
We studied non-small cell lung cancers (NSCLC) from 60 patients for abnormalities in the c-erbB-2 gene. Eleven human lung cancer cell lines, including four derived from small cell lung cancer (SCLC) and seven derived from NSCLC were also examined for altered c-erbB-2 gene expression. Southern blot analysis of paired tumor and normal lung samples demonstrated that amplification of the c-erbB-2 gene is rare in NSCLC (2/60) and not restricted to adenocarcinomas. One patient showed an EcoRI restriction fragment length polymorphism for the c-erbB-2 locus. Four of four SCLC cell lines demonstrated minimal or nondetectable expression of c-erbB-2 mRNA compared to high levels of expression by seven of seven NSCLC lines. The highest expression levels were seen in four of four adenocarcinomas. We conclude that c-erbB-2 expression is different in SCLC compared to NSCLC and high expression of c-erbB-2 is consistently present in lung adenocarcinomas.
Schneider
P M
PM
Department of Thoracic Surgery, University of Texas, M.D. Anderson Cancer Center, Houston 77030.
Hung
M C
MC
Chiocca
S M
SM
Manning
J
J
Zhao
X Y
XY
Fang
K
K
Roth
J A
JA
D015152:Blotting, Northern; D015139:Blotting, Southern; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D018288:Carcinoma, Small Cell / Q000235:genetics*; D002460:Cell Line; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D005784:Gene Amplification; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D008207:Lymphatic Metastasis; D012150:Polymorphism, Restriction Fragment Length; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D011519:Proto-Oncogenes; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D018719:Erb-b2 Receptor Tyrosine Kinases; D014158:Transcription, Genetic
D004273:DNA, Neoplasm; D011518:Proto-Oncogene Proteins; D012334:RNA, Neoplasm; D018719:Erb-b2 Receptor Tyrosine Kinases