A rapid method for detection of flavivirus antigens: staphylococcal co-agglutination test using monoclonal antibodies to Japanese encephalitis virus.
Staphylococcus aureus rich in protein A when coated with monoclonal antibodies (MoAb) to Japanese encephalitis virus (JEV) gave a highly specific reaction with flavivirus antigens. The bacteria coated with JEV species-specific MoAb gave a strong co-agglutination with fifty-six JEV isolates from various parts of China, but no co-agglutination with Murray Valley encephalitis (MVE) and Kunjin (Kun) virus antigens. The flavivirus- and subgroup-specific MoAbs were reactive with MVE and Kun, as well as with the majority of the JEV strains. Blocking test with homologous MoAbs abolished co-agglutination further confirming its specificity. Numerous virus particles were observed on the surface of MoAb-coated staphylococci under the electron microscope after co-agglutination. The test appeared rapid, specific, simple to perform, and useful for rapid detection and identification of flaviviruses.
Zhang
Y H
YH
Institute of Virology, Chinese Academy of Preventive Medicine, Beijing.
Yu
W F
WF
Cai
J
J
Qian
D
D
Zhao
T X
TX
Xu
Z Z
ZZ
Wan
M X
MX
D000372:Agglutination Tests; D000911:Antibodies, Monoclonal / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology; D002462:Cell Membrane / Q000276:immunology; D003429:Cross Reactions; D004664:Encephalitis Virus, Japanese / Q000276:immunology; D005416:Flavivirus / Q000276:immunology / Q000302:isolation & purification*; D006385:Hemagglutination Inhibition Tests; D006863:Hydrogen-Ion Concentration; D009500:Neutralization Tests; D012680:Sensitivity and Specificity; D013211:Staphylococcus aureus / Q000276:immunology
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral