Nick translation of lambda phage DNA with a deoxycytidine analog spin labeled in the 5 position.
The synthesis and properties of a novel C(5)-spin-labeled 2'-deoxycytidine 5'-triphosphate which serves as a suitable substrate for the template-directed enzyme Escherichia coli DNA polymerase I are reported. The spin label is readily incorporated into lambda phage DNA by nick translation where it reports the characteristic local base motion for double- and single-stranded DNA as determined by electron spin resonance. The high-frequency deoxycytidine motion is similar to the previously reported thymidine motion in double-stranded lambda phage DNA.
Strobel
O K
OK
Department of Chemistry, University of Cincinnati, Ohio 45221.
Bobst
E V
EV
Bobst
A M
AM
D010582:Bacteriophage lambda / Q000235:genetics*; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D004279:DNA, Viral / Q000235:genetics*; D003841:Deoxycytidine / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D014176:Protein Biosynthesis
D004279:DNA, Viral; D003841:Deoxycytidine