Centrifugal elutriation of hepatocytes from 2-acetylaminofluorene-treated rats and their characterization by flow cytometry.
Treatment of male Wistar rats with 2-acetylaminofluorene (2-AAF) markedly altered the ploidy distribution of liver cells. Small diploid hepatocytes first appeared after 4-5 weeks feeding of a diet containing 0.02% 2-AAF; after 9 weeks 65-70% of the hepatocytes were diploid. Approximately two-thirds of this new liver cell population persisted after termination of the treatment. The hepatocytes from 2-AAF treated animals were separated according to size and ploidy by centrifugal elutriation and stained for gamma-glutamyltranspeptidase (gamma-GTase). The percentage of gamma-GTase-positive hepatocytes did not significantly differ between the various elutriated cell fractions. Thus gamma-GTase-positive liver cells obtained by feeding of 2-AAF do not represent a distinct size class of hepatocytes. The significance of carcinogen-induced diploid hepatocytes in hepatocarcinogenesis is discussed.
Klose
U
U
GSF-Institut für Toxikologie, Neuherberg, FRG.
Thierau
D
D
Greim
H
H
Schwarz
L R
LR
D015073:2-Acetylaminofluorene / Q000633:toxicity*; D000818:Animals; D002498:Centrifugation; D004247:DNA / Q000032:analysis; D005434:Flow Cytometry; D008099:Liver / Q000032:analysis / Q000187:drug effects*; D008297:Male; D011003:Ploidies; D051381:Rats; D011919:Rats, Inbred Strains; D005723:gamma-Glutamyltransferase / Q000032:analysis
D004247:DNA; D015073:2-Acetylaminofluorene; D005723:gamma-Glutamyltransferase