title: Kinetic resolution of racemic leukotriene A4 by mammalian cytosolic epoxide hydrolases.
abstract: Cytosols of rat and guinea pig liver and of human placenta were screened for their capacity to catalyze the conversion of racemic leukotriene A4 into 5S, 12R-dihydroxy-(Z,E,E,Z)-6,8,10,14-eicosatetraenoic acid (leukotriene B4). The epoxide hydrolase activities showed some specificity for the 5S,6S-oxido-(E,E,Z,Z)-7,9,11,14-eicosatetraenoic acid (LTA4) and produced mixtures of leukotriene B4 and its enantiomer containing up to 78-87% of leukotriene B4.
authors.affiliation: Laboratoire de Chimie Organique Enzymatique, Université des Sciences et Techniques de Languedoc, Montpellier, France.
authors.forename: G
authors.initials: G
authors.lastname: Maury
authors.forename: E
authors.initials: E
authors.lastname: Ginestar
authors.forename: H
authors.initials: H
authors.lastname: Lopez-Parrot
authors.forename: H
authors.initials: H
authors.lastname: Thaler-Dao
authors.forename: A
authors.initials: A
authors.lastname: Crastes De Paulet
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003600:Cytosol / Q000201:enzymology; D004851:Epoxide Hydrolases / Q000378:metabolism*; D005260:Female; D006168:Guinea Pigs; D007700:Kinetics; D017572:Leukotriene A4; D007975:Leukotriene B4 / Q000378:metabolism*; D015289:Leukotrienes / Q000378:metabolism*; D008099:Liver / Q000201:enzymology* / Q000378:metabolism; D010920:Placenta / Q000201:enzymology* / Q000378:metabolism; D011247:Pregnancy; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity
chemical_list: D017572:Leukotriene A4; D015289:Leukotrienes; D007975:Leukotriene B4; D004851:Epoxide Hydrolases