Evidence that the leukocyte-common antigen is required for antigen-induced T lymphocyte proliferation.
The leukocyte-common antigen (L-CA) is a family of large molecular weight glycoproteins uniquely expressed on the surface of all nucleated cells of hematopoietic origin. The glycoprotein consists of a heavily glycosylated exterior domain, a single membrane spanning region, and a large cytoplasmic domain that contains tyrosine phosphatase activity. To investigate the function of this family, we generated T cell clones that lacked L-CA (L-CA-). The expression of the alpha beta T cell receptor, CD3, CD4, IL-2 receptor (p55), LFA-1, Thy-1, and Pgp-1 (CD44) was normal. The L-CA- T cell clones failed to proliferate in response to antigen or cross-linked CD3; however, they could still proliferate in response to IL-2. An L-CA+ revertant was obtained and the ability to proliferate in response to antigen and cross-linked CD3 was restored. These data indicate that L-CA is required for T cells to enter into cell cycle in response to antigen.
Pingel
J T
JT
Department of Pathology, Washington University School of Medicine, St. Louis, Missouri 63110.
Thomas
M L
ML
D000595:Amino Acid Sequence; D000818:Animals; D000943:Antigens, Differentiation / Q000235:genetics / Q000276:immunology*; D015152:Blotting, Northern; D002460:Cell Line; D002999:Clone Cells; D005434:Flow Cytometry; D006649:Histocompatibility Antigens / Q000235:genetics / Q000276:immunology*; D006801:Humans; D017493:Leukocyte Common Antigens; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000276:immunology*; D051379:Mice; D008808:Mice, Inbred CBA; D008969:Molecular Sequence Data; D010749:Phosphoprotein Phosphatases / Q000235:genetics; D017027:Protein Tyrosine Phosphatases; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D015375:Receptors, Interleukin-2 / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology*
D000943:Antigens, Differentiation; D006649:Histocompatibility Antigens; D008562:Membrane Glycoproteins; D015375:Receptors, Interleukin-2; D011505:Protein-Tyrosine Kinases; D010749:Phosphoprotein Phosphatases; D017493:Leukocyte Common Antigens; D017027:Protein Tyrosine Phosphatases