Augmentation of the generation of lymphokine-activated killer cells after a single dose of mitomycin C in cancer patients.
The effect of mitomycin C administration on the generation of cytotoxic cells, induced by in vitro activation of peripheral blood mononuclear cells (PBM) with interleukin-2, was studied in patients with various carcinomas. The ability of PBM to generate lymphokine-activated killer (LAK) cell activity against Raji cell targets was significantly augmented 5 and 7 days after a single intravenous dose of 12 mg/m2 mitomycin C, when compared to that of PBM obtained before mitomycin C injection. Further, LAK cell activity against autologous tumor cells was also significantly increased after the drug administration. The distribution of lymphocyte subsets exhibited a significant increase in the percentage of CD3+ cells after injection, with the elevation of the CD4/CD8 ratio. Furthermore, the proportion of the CD4+ Leu8+ subpopulation, which identifies inducers of suppression, was significantly reduced. Thus, the decrease in the proportion of suppressor-inducer subsets of PBM might be at least partially, responsible for the augmented generation of LAK cells after mitomycin C administration.
Nanbara
S
S
Department of Surgery, Kyushu University, Beppu, Japan.
Arinaga
S
S
Akiyoshi
T
T
D000943:Antigens, Differentiation / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000145:classification; D016827:CD8 Antigens; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D006801:Humans; D007111:Immunity, Cellular / Q000187:drug effects; D007167:Immunotherapy; D066298:In Vitro Techniques; D007694:Killer Cells, Natural / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000145:classification / Q000276:immunology; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000494:pharmacology; D016685:Mitomycin; D008937:Mitomycins / Q000008:administration & dosage*; D009369:Neoplasms / Q000628:therapy*; D014407:Tumor Cells, Cultured
D000943:Antigens, Differentiation; D000945:Antigens, Differentiation, T-Lymphocyte; D016827:CD8 Antigens; D008222:Lymphokines; D008937:Mitomycins; D016685:Mitomycin