title: Inactivation of rat gastric mucosal histidine decarboxylase by phosphatase.
abstract: Histidine decarboxylase of supernatants as well as of purified preparations from rat gastric mucosa is inactivated by a non-specific phosphatase in the absence of pyridoxal 5'-phosphate. The inactivation is a time and concentration-dependent process. Pyridoxal 5'-phosphate, but not histidine, protects the enzyme against phosphatase action. The inactivation is reversible, only pyridoxal 5'-phosphate reactivates the inactivated enzyme. Pyridoxamine 5'-phosphate is ineffective for histidine decarboxylase, but is converted into an active coenzyme only in gastric supernatant. Evidence for the occurrence of an active phosphatase in gastric tissue is also presented; its properties are those of an acid phosphatase and are similar to those of phosphatases hydrolyzing pyridoxal 5'-phosphate in other tissues. The data indicate that phosphatase promotes apoenzyme formation and may play a role in the regulation of histamine synthesis.
authors.affiliation: Laboratoire de Chimie Biologique, INSERM U. 205, Institut National des Sciences Appliquées, Villeurbanne, France.
authors.forename: A
authors.initials: A
authors.lastname: Savany
authors.forename: L
authors.initials: L
authors.lastname: Cronenberger
mesh_terms: D000818:Animals; D002262:Carboxy-Lyases / Q000037:antagonists & inhibitors*; D005753:Gastric Mucosa / Q000201:enzymology*; D006640:Histidine Decarboxylase / Q000037:antagonists & inhibitors*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D010710:Phosphates / Q000494:pharmacology; D010744:Phosphoric Monoester Hydrolases / Q000037:antagonists & inhibitors / Q000502:physiology*; D051381:Rats
chemical_list: D010710:Phosphates; D010744:Phosphoric Monoester Hydrolases; D002262:Carboxy-Lyases; D006640:Histidine Decarboxylase