Spectrophotometric determination of penicilloates in penicillins.
A procedure has been developed for the determination of penicilloates based on the reduction of CuII and detection of CuI using neocuproine. The sensitivity range is 5.6-56 microns penicilloate and the standard deviation was found to be between 1 and 2%. All the penicilloates tested reduced CuII under the conditions of the assay and a slight modification allowed a reasonably accurate determination of the level of this biologically inactive ingredient in a mixture with the parent penicillin. The deterioration of solutions of benzylpenicillin was also studied.
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D003300:Copper; D010084:Oxidation-Reduction; D010397:Penicillanic Acid / Q000032:analysis* / Q000031:analogs & derivatives; D010406:Penicillins / Q000032:analysis*; D010618:Phenanthrolines; D013053:Spectrophotometry
D010406:Penicillins; D010618:Phenanthrolines; C008782:penicilloic acid; D003300:Copper; D010397:Penicillanic Acid; C002701:neocuproine