A common site for immortalizing proviral integrations in Friend erythroleukemia: molecular cloning and characterization.
By using a tagged derivative of Friend spleen focus-forming virus, we previously obtained evidence that proviral integration(s) in the host genome can cause erythroblast immortality by abrogating the commitment of the cell to differentiate (C. Spiro, B. Gliniak, and D. Kabat, J. Virol. 62:4129-4135, 1988). Exploiting the fact that each leukemia was a single clone that contained one tagged provirus, we have now molecularly cloned and characterized one common genomic site for immortalizing proviral integrations.
Paul
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Department of Biochemistry, School of Medicine, Oregon Health Sciences University, Portland 97201-3098.
Schuetze
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Kozak
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Kabat
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D000818:Animals; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D005622:Friend murine leukemia virus / Q000235:genetics*; D005814:Genes, Viral; D009052:Leukemia Virus, Murine / Q000235:genetics*; D004915:Leukemia, Erythroblastic, Acute / Q000382:microbiology*; D008242:Lysogeny; D051379:Mice; D015183:Restriction Mapping; D013154:Spleen / Q000382:microbiology; D013155:Spleen Focus-Forming Viruses / Q000235:genetics* / Q000302:isolation & purification
D004279:DNA, Viral