A trial to improve the analysis of boron in biological materials.
For the spectrophotometric determination of boron in biological materials, two decomposition methods were used. One is the alkali fusion method, and the other is the acid decomposition method using a uniseal decomposition vessel. A long-range calibration curve for the former method was obtained by using an ultrapure sulfuric acid. The alkali fusion method was applied to samples into which paraboronophenylalanine (BPA) or its hydrochloric salt (BPA.HCl) had been injected. By the acid decomposition method using a uniseal vessel, 50 mg dried liver was digested by a mixed solution of 1 ml conc. sulfuric acid and 6 ml 30% hydrogen peroxide. A problem exists, however, in that the hydrogen peroxide remaining in the decomposition solution must be eliminated without loss of boron.
Yoshino
K
K
Department of Chemistry, Faculty of Science, Shinshu University, Matsumoto, Japan.
Kajiyama
Y
Y
Honda
T
T
Mori
Y
Y
Honda
C
C
Ichihashi
M
M
Mishima
Y
Y
D000468:Alkalies / Q000378:metabolism; D000818:Animals; D001895:Boron / Q000032:analysis*; D006861:Hydrogen Peroxide; D007668:Kidney / Q000032:analysis / Q000378:metabolism; D008099:Liver / Q000032:analysis / Q000378:metabolism; D008546:Melanoma, Experimental / Q000032:analysis / Q000378:metabolism; D008722:Methods; D009566:Nitrates; D017942:Nitric Acid; D051381:Rats; D012878:Skin Neoplasms / Q000032:analysis / Q000378:metabolism; D013053:Spectrophotometry / Q000379:methods
D000468:Alkalies; D009566:Nitrates; D017942:Nitric Acid; D006861:Hydrogen Peroxide; D001895:Boron