The unexpected antitermination of gyrA-directed transcripts is enhanced by DNA relaxation.
We show that transcription originating at the gyrA promoter of Escherichia coli is less subject to termination at the lambda Toop terminator (22% read-through) than is transcription originating from either the galOP (1% read-through) or topA (3% read-through) control regions. Furthermore, termination of the gyrA transcripts decreases (60% read-through) upon relaxation of the template DNA. We believe that events related to stimulation of transcription at the gyrA promoter by DNA relaxation are responsible for the enhanced terminator read-through.
Carty
M
M
E.I. du Pont de Nemours, Central Research and Development Division, Wilmington, DE 19880-0328.
Menzel
R
R
D001426:Bacterial Proteins / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics / Q000648:ultrastructure; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D007700:Kinetics; D010957:Plasmids; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000235:genetics
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D001616:beta-Galactosidase