Neurite outgrowth from mouse neuroblastoma and cerebellar cells induced by the protein kinase inhibitor H-7.
The protein kinase inhibitor H-7 (1-(5-isoquinolinesulfonyl)-2-methylpiperazine dihydrochloride) or staurosporine was found to induce neurite outgrowth from mouse neuroblastoma N18TG2 cells and cultured cerebellar cells by light microscopy. Examination by electron microscopy revealed some morphological differences between the neurites of N18TG2 cells induced by H-7 and dB-cAMP, in which H-7 formed longer and thinner neurites and more abundant localized varicose structures along neurites than dB-cAMP. These results indicate that protein kinase inhibitor itself can act as a potent stimulus for inducing neurite outgrowth in a different manner from dB-cAMP.
Tsuda
M
M
Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Ono
K
K
Katayama
N
N
Yamagata
Y
Y
Kikuchi
K
K
Tsuchiya
T
T
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002525:Cerebellar Cortex / Q000187:drug effects* / Q000648:ultrastructure; D007546:Isoquinolines / Q000494:pharmacology*; D009447:Neuroblastoma / Q000378:metabolism* / Q000648:ultrastructure; D009474:Neurons / Q000187:drug effects* / Q000648:ultrastructure; D010879:Piperazines / Q000494:pharmacology*
D007546:Isoquinolines; D010879:Piperazines; D003994:Bucladesine; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine