Two types of K+ channels in the apical membrane of rabbit proximal tubule in primary culture.
The patch-clamp technique was used to investigate ionic channels in the apical membrane of rabbit proximal tubule cells in primary culture. Cell-attached recordings revealed the presence of a highly selective K+ channel with a conductance of 130 pS. The channel activity was increased with membrane depolarization. Experiments performed on excised patches showed that the channel activity depended on the free Ca2+ concentration on the cytoplasmic face of the membrane and that decreasing the cytoplasmic pH from 7.2 to 6.0 also decreased the channel activity. In symmetrical 140 mM KCl solutions the channel conductance was 200 pS. The channel was blocked by barium, tetraethylammonium and Leiurus quinquestriatus scorpion venom (from which charybdotoxin is extracted) when applied to the extracellular face of the channel. Barium and quinidine also blocked the channel when applied to the cytoplasmic face of the membrane. Another K+ channel with a conductance of 42 pS in symmetrical KCl solutions was also observed in excised patches. The channel was blocked by barium and apamin, but not by tetraethylammonium applied to the extracellular face of the membrane. Using the whole-cell recording configuration we determined a K+ conductance of 4.96 nS per cell that was blocked by 65% when 10 mM tetraethylammonium was applied to the bathing medium.
Merot
J
J
INSERM U 246, Département Biologie/SBCe, Gif Sur Yvette, France.
Bidet
M
M
Le Maout
S
S
Tauc
M
M
Poujeol
P
P
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001464:Barium / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D002462:Cell Membrane / Q000502:physiology; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000096:biosynthesis; D004553:Electric Conductivity; D006593:Hexokinase / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007687:Kidney Tubules, Proximal / Q000187:drug effects / Q000502:physiology*; D007700:Kinetics; D007931:Leucyl Aminopeptidase / Q000378:metabolism; D008564:Membrane Potentials; D010281:Parathyroid Hormone / Q000494:pharmacology; D015221:Potassium Channels / Q000187:drug effects / Q000502:physiology*; D011802:Quinidine / Q000494:pharmacology; D011817:Rabbits; D012604:Scorpion Venoms / Q000494:pharmacology; D019789:Tetraethylammonium; D013757:Tetraethylammonium Compounds / Q000494:pharmacology; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D010281:Parathyroid Hormone; D015221:Potassium Channels; D012604:Scorpion Venoms; D013757:Tetraethylammonium Compounds; D001464:Barium; D019789:Tetraethylammonium; D000242:Cyclic AMP; D005723:gamma-Glutamyltransferase; D006593:Hexokinase; D000469:Alkaline Phosphatase; D007931:Leucyl Aminopeptidase; D011802:Quinidine; D002118:Calcium