Inhibition of chemical Epstein-Barr virus induction by dimethyl sulfoxide and related polar compounds.
Dimethyl sulfoxide and twelve related polar compounds, capable of inducing differentiation of murine erythroleukemia cells, markedly inhibited induction of Epstein-Barr virus antigens in virus producer and nonproducer lymphoblastoid cells exposed to 12-O-tetradecanoylphorbol-13-acetate, n-butyric acid, 5-iodo-2'-deoxyuridine, and a combination of 12-O-tetradecanoylphorbol-13-acetate and n-butyric acid. The concentration at which these compounds caused 90% inhibition of antigen induction corresponded to that optimal for induction of differentiation. At the same concentrations, polar compounds did not affect cell viability or virus induction by superinfection with Epstein-Barr virus recovered from P3HR-1 cells. The inhibitory activity was reversible. Three nonpolar differentiating compounds revealed no inhibitory activity.
Yanase
S
S
Department of Microbiology, Faculty of Medicine, Kyoto University, Japan.
Sugawara
K
K
D000081:Acetamides / Q000494:pharmacology; D000956:Antigens, Viral / Q000096:biosynthesis; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004854:Herpesvirus 4, Human / Q000187:drug effects* / Q000254:growth & development / Q000276:immunology; D007065:Idoxuridine / Q000494:pharmacology; D007942:Leukemia, Experimental; D008782:Methylurea Compounds / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014775:Virus Activation / Q000187:drug effects*
D000081:Acetamides; D000956:Antigens, Viral; D002087:Butyrates; D008782:Methylurea Compounds; D020148:Butyric Acid; C004168:1,1,3,3-tetramethylurea; C013959:dimethylacetamide; D007065:Idoxuridine; D013755:Tetradecanoylphorbol Acetate; D004121:Dimethyl Sulfoxide