title: Bluetongue virus-17 fusion protein ns1 expressed in Escherichia coli by pUC vectors.
abstract: The cDNA coding for the major nonstructural protein, NS1, of bluetongue serotype 17 (BTV-17) was cloned previously. Using pUC plasmids, we have successfully expressed the NS1 protein in Escherichia coli as a LacZ-NS1 fusion protein. The recombinant NS1 protein reacted with rabbit anti-BTV-17 antiserum, and was thus immunologically indistinguishable from the native BTV-17 NS1 protein. This was the first bluetongue viral protein to be produced in a bacterial system.
authors.affiliation: Department of Biochemistry & Biophysics, University of California, Davis 95616.
authors.forename: L F
authors.initials: LF
authors.lastname: Wang
authors.forename: R H
authors.initials: RH
authors.lastname: Doi
authors.forename: L F
authors.initials: LF
authors.lastname: Chuang
authors.forename: B I
authors.initials: BI
authors.lastname: Osburn
authors.forename: J
authors.initials: J
authors.lastname: Maisonnave
authors.forename: E
authors.initials: E
authors.lastname: Benjamini
authors.forename: R Y
authors.initials: RY
authors.lastname: Chuang
mesh_terms: D015153:Blotting, Western; D001820:Bluetongue virus / Q000235:genetics*; D002213:Capsid / Q000096:biosynthesis / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D010957:Plasmids; D011994:Recombinant Proteins; D012087:Reoviridae / Q000235:genetics*; D014758:Viral Core Proteins / Q000096:biosynthesis / Q000235:genetics*; D014760:Viral Fusion Proteins / Q000096:biosynthesis / Q000235:genetics*; D017361:Viral Nonstructural Proteins
chemical_list: D004274:DNA, Recombinant; D011994:Recombinant Proteins; D014758:Viral Core Proteins; D014760:Viral Fusion Proteins; D017361:Viral Nonstructural Proteins; D004262:DNA Restriction Enzymes