The immunophenotype of Reed-Sternberg cells. A study of 50 cases of Hodgkin's disease using fixed frozen tissues.
The authors analyzed 50 cases of Hodgkin's disease (HD) with a panel of antibodies which detect B-cell and T-cell specific markers and activation antigens using a sensitive immunocytochemical technique and paraformaldehyde-lysine-periodate (PLP) fixed-frozen tissues. In 60% of cases either T-cell or B-cell specific antigens were detected on Reed-Sternberg (RS) cells. Most T-cell cases were of nodular sclerosing (NS) and mixed cellularity (MC) type (65% and 30%, respectively) and most B-cell cases were either of NS or lymphocyte predominant (LP) type (55% and 45%, respectively). Leukocyte common antigen (LCA) was usually negative on RS cells in NS, but was present in approximately 50% of the cases of MC and LP types. Almost all cases were positive for the CD30 antigen (Ki-1). Most cases were also positive for CD15 (LeuM1) with the exception of the LP type. Activation antigens (Ia, CD25, T9) were expressed in a high proportion of cases regardless of subtype. The results suggest that most cases of HD are histogenetically derived from activated T-cells or B-cells.
Agnarsson
B A
BA
Department of Pathology, Beth Israel Hospital, Harvard Medical School, Boston, MA 02215.
Kadin
M E
ME
D000293:Adolescent; D000328:Adult; D000368:Aged; D000943:Antigens, Differentiation / Q000032:analysis*; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis*; D002648:Child; D005260:Female; D005629:Frozen Sections; D006644:Histiocytes / Q000276:immunology*; D006649:Histocompatibility Antigens / Q000032:analysis; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006689:Hodgkin Disease / Q000276:immunology / Q000473:pathology*; D006801:Humans; D007124:Immunoenzyme Techniques; D017730:Ki-1 Antigen; D017493:Leukocyte Common Antigens; D016256:Lewis X Antigen; D008297:Male; D008875:Middle Aged; D015375:Receptors, Interleukin-2 / Q000032:analysis
D000943:Antigens, Differentiation; D015214:Antigens, Differentiation, Myelomonocytic; D000951:Antigens, Neoplasm; D006649:Histocompatibility Antigens; D000949:Histocompatibility Antigens Class II; D017730:Ki-1 Antigen; D016256:Lewis X Antigen; D015375:Receptors, Interleukin-2; D017493:Leukocyte Common Antigens