Isolation of a cDNA likely to encode a novel Ca2+-dependent/calmodulin-stimulated protein phosphatase.
Complementary DNA encoding a novel protein phosphatase catalytic subunit has been isolated from a rabbit brain library. The deduced protein sequence is more similar to the major Ca2+-dependent/calmodulin-stimulated protein phosphatase (2B) in brain (55% identity) than to protein phosphatases 1 and 2A (38-39% identity). A putative calmodulin-binding domain is present C-terminal to the catalytic domain, which closely resembles that of the mouse brain enzyme. These findings represent the first indication that at least two distinct Ca2+-dependent/calmodulin-stimulated protein phosphatases are present in mammalian brain.
da Cruz e Silva
E F
EF
Department of Biochemistry, Medical Sciences Institute, University of Dundee, U.K.
Cohen
P T
PT
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019703:Calcineurin; D002118:Calcium / Q000378:metabolism*; D002148:Calmodulin-Binding Proteins / Q000235:genetics*; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010749:Phosphoprotein Phosphatases / Q000235:genetics*; D011817:Rabbits
D002148:Calmodulin-Binding Proteins; D004247:DNA; D019703:Calcineurin; D010749:Phosphoprotein Phosphatases; D002118:Calcium