Drosophila melanogaster strand transferase. A protein that forms heteroduplex DNA in the absence of both ATP and single-strand DNA binding protein.
The purification of a Drosophila strand transfer protein is described, which involves Bio-Rex 70, Superose 6, Mono S, and single-stranded DNA-agarose chromatography. A 105,000-dalton polypeptide copurifies with the strand transfer activity on the last two column steps. The strand transferase carries out strand transfer at an unusually low protein:single-stranded DNA ratio and requires neither a nucleotide cofactor nor exogenous single-strand DNA binding protein to form heteroduplex DNA. Biochemical analysis of the reaction products has established that one strand of the DNA duplex is displaced during the reaction. Several properties, including the kinetics and stoichiometry of strand transfer, differentiate this activity from previously characterized strand transferases.
Lowenhaupt
K
K
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Sander
M
M
Hauser
C
C
Rich
A
A
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004247:DNA / Q000096:biosynthesis*; D004268:DNA-Binding Proteins / Q000378:metabolism; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000201:enzymology*; D007700:Kinetics; D008970:Molecular Weight; D009692:Nucleic Acid Heteroduplexes / Q000096:biosynthesis*; D009713:Nucleotidyltransferases / Q000302:isolation & purification / Q000378:metabolism*
D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D009692:Nucleic Acid Heteroduplexes; D000255:Adenosine Triphosphate; D004247:DNA; D009713:Nucleotidyltransferases; C061900:Rrp1 protein, Drosophila