Blockade of the late IPSP in rat CA1 hippocampal neurons by 2-hydroxy-saclofen.
The effects of the GABAB receptor antagonist 2-hydroxy-saclofen were studied using intracellular recording of synaptic potential from CA1 hippocampal neurons. 2-Hydroxy-saclofen (50-200 microM) reversibly blocked the late, GABAB receptor-mediated inhibitory postsynaptic potential (IPSP) but not the early, GABAA receptor-mediated IPSP. In addition, the hyperpolarizing response to baclofen was reduced by similar concentrations of 2-hydroxy-saclofen. This suggests that 2-hydroxy-saclofen is a potent antagonist at postsynaptic GABAB receptors on hippocampal neurons.
Lambert
N A
NA
Department of Neurobiology, Northeastern Ohio Universities College of Medicine, Rootstown 44272.
Harrison
N L
NL
Kerr
D I
DI
Ong
J
J
Prager
R H
RH
Teyler
T J
TJ
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D001418:Baclofen / Q000031:analogs & derivatives* / Q000494:pharmacology; D058787:GABA-A Receptor Antagonists; D006624:Hippocampus / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D009433:Neural Inhibition / Q000187:drug effects*; D051381:Rats; D011963:Receptors, GABA-A / Q000502:physiology*
D058787:GABA-A Receptor Antagonists; D011963:Receptors, GABA-A; D001418:Baclofen; C057491:2-hydroxysaclofen