Association of Tat protein and viral mRNA with nuclear matrix from HIV-1-infected H9 cells.
The transactivating protein from human immunodeficiency virus type 1 (HIV-1), Tat, was found to bind to the nuclear matrix from uninfected and HIV-1-infected H9 cells. Addition of the Zn2+, Cd2+ and Cu2+ chelator o-phenanthroline destroyed the matrix fibrils and the binding affinity of Tat to the matrix. A sequential treatment of the matrix, first with o-phenanthroline and then with ZnCl2, partially restored the fibrillar-like matrix structure. Infection of H9 cells with HIV-1 resulted in a displacement of cellular mRNA by viral mRNA from the nuclear matrix. Both the matrix-bound host cell and HIV-1 mRNA were found to dissociate from the matrix in the presence of o-phenanthroline. This could be prevented by coincubation with Zn2+ or Cu2+ (but not Mg2+), which stabilize the mRNA containing nuclear matrix structure.
Müller
W E
WE
Abteilung Angewandte Molekularbiologie, Universität, Mainz, F.R.G.
Wenger
R
R
Reuter
P
P
Renneisen
K
K
Schröder
H C
HC
D002467:Cell Nucleus / Q000378:metabolism*; D002478:Cells, Cultured; D002614:Chelating Agents / Q000494:pharmacology; D015342:DNA Probes; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005456:Fluorescent Dyes; D015696:Gene Products, tat; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism*; D008667:Metalloproteins / Q000378:metabolism; D008856:Microscopy, Fluorescence; D010618:Phenanthrolines / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism*; D013861:Thiocyanates; D014157:Transcription Factors / Q000378:metabolism*; D054322:tat Gene Products, Human Immunodeficiency Virus
D002614:Chelating Agents; D015342:DNA Probes; D005452:Fluoresceins; D005456:Fluorescent Dyes; D015696:Gene Products, tat; D008667:Metalloproteins; D010618:Phenanthrolines; D012333:RNA, Messenger; D012367:RNA, Viral; D013861:Thiocyanates; D014157:Transcription Factors; D054322:tat Gene Products, Human Immunodeficiency Virus; D016650:Fluorescein-5-isothiocyanate; C025205:1,10-phenanthroline