Immune response to immunostimulatory complexes (ISCOMs) prepared from human immunodeficiency virus type 1 (HIV-1) or the HIV-1 external envelope glycoprotein (gp120).
In mice, immunostimulatory complexes (ISCOMs) prepared from HIV-1 B external envelope glycoprotein (gp120) induced 10-fold higher antibody titres than gp120 emulsified in depot adjuvant, as measured by enzyme-linked immunosorbent assay (ELISA). Rhesus monkeys immunized with gp120 ISCOMs produced precipitating and virus neutralizing antibody titres equivalent to those seen in HIV-infected chimpanzees and humans. After multiple immunizations with HIV-1 B gp120 ISCOMs, a rhesus monkey developed a neutralizing response to the HIV-1 isolates RF and MN, but not to the CC isolate. Antisera from ISCOM-immunized rhesus monkeys recognized gp120 on the membranes of HIV-1 B-infected H9 cells, indicating the preservation of epitope structure in the ISCOMs matrix.
Pyle
S W
SW
Program Resources, Inc., National Cancer Institute-Frederick Cancer Research Facility, MD 21701.
Morein
B
B
Bess
J W
JW
Akerblom
L
L
Nara
P L
PL
Nigida
S M
SM
Lerche
N W
NW
Robey
W G
WG
Fischinger
P J
PJ
Arthur
L O
LO
D000276:Adjuvants, Immunologic; D000818:Animals; D015153:Blotting, Western; D002499:Centrifugation, Density Gradient; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D015483:HIV Antibodies / Q000096:biosynthesis*; D015699:HIV Envelope Protein gp120 / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D008253:Macaca mulatta; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D009500:Neutralization Tests; D011817:Rabbits; D011863:Radioimmunoassay
D000276:Adjuvants, Immunologic; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120