Soluble IL-2 receptor in AIDS. Correlation of its serum level with the classification of HIV-induced diseases and its characterization.
By using a fluorescence sandwich ELISA, elevated IL-2R levels were detected in the sera from both HIV-infected hemophiliacs and other HIV-infected patients. The serum IL-2R levels were reflective of the classification of HIV-induced diseases by the Centers for Disease Control. Moreover, the IL-2R levels were negatively correlated most prominently with CD4 cell counts, with lymphocyte counts, and with a decrease in the CD4-CD8 ratio but not with either WBC counts or B cell counts. As striking elevations of serum IL-2R were noted in AIDS patients with group IVD infection, the serum IL-2R was purified sequentially by using size-exclusion HPLC, high-pressure chromatofocusing, and H48 affinity HPLC. The isoelectric point values of IL-2R were separated into 4.2 and 3.8, whereas the Mr was determined to be only 45 kDa by immunoprecipitation with H48 antibody followed by SDS-PAGE. However, production of cellular and supernatant IL-2R was not elevated in PBMC of patients with AIDS or in any of the 19 HIV-I- or HIV-II-infected cell line cells. In contrast, PBMC from patients with adult T cell leukemia and cell line cells that expressed human T cell lymphotropic virus -I or -II produced soluble IL-2R, constitutively. The mechanisms by which serum levels of IL-2R might be elevated in HIV-infected patients are discussed in comparison with that in adult T cell leukemia patients.
Honda
M
M
Department of Cellular Immunology, National Institute of Health, Tokyo, Japan.
Kitamura
K
K
Matsuda
K
K
Yokota
Y
Y
Yamamoto
N
N
Mitsuyasu
R
R
Chermann
J C
JC
Tokunaga
T
T
D000163:Acquired Immunodeficiency Syndrome / Q000097:blood / Q000145:classification* / Q000209:etiology; D002460:Cell Line; D006800:Deltaretrovirus Infections / Q000097:blood / Q000145:classification* / Q000209:etiology; D006467:Hemophilia A / Q000097:blood / Q000150:complications / Q000276:immunology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D015375:Receptors, Interleukin-2 / Q000032:analysis* / Q000096:biosynthesis / Q000378:metabolism; D012995:Solubility
D015375:Receptors, Interleukin-2