Growth-inhibitory effect of TGF-B on human fetal adrenal cells in primary monolayer culture.
We examined the effects of transforming-growth factor-B (TGF-B) on growth ([3H]-thymidine uptake) and function (dehydroepiandrosterone sulfate [DHAS] and cortisol production) of human fetal zone adrenal cells. Results indicate that TGF-B significantly inhibits, in a dose-related manner, both basal and epidermal growth factor (EGF)-stimulated cell growth: IC50 = 0.1-0.25 ng/ml. EGF is ineffective in overcoming the inhibitory effect of TGF-B, suggesting a noncompetitive antagonism between the two factors. Also, the inhibitory effect of TGF-B is additive to that of adrenocorticotropic hormone (ACTH). On the other hand, TGF-B (1 ng/ml) does not significantly change basal or ACTH-stimulated DHAS or cortisol secretion. We conclude that, unlike its effect on other steroid-producing cells, TGF-B inhibits growth of fetal zone cells and does not appear to have a significant inhibitory effect on steroidogenesis.
Riopel
L
L
McGill University-Montreal Children's Hospital Research Institute, Quebec, Canada.
Branchaud
C L
CL
Goodyer
C G
CG
Adkar
V
V
Lefebvre
Y
Y
D000311:Adrenal Glands / Q000166:cytology* / Q000187:drug effects / Q000196:embryology; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005333:Fetus / Q000166:cytology; D006801:Humans; D006854:Hydrocortisone / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism / Q000493:pharmacokinetics; D015291:Transforming Growth Factors / Q000494:pharmacology*
D004815:Epidermal Growth Factor; D015291:Transforming Growth Factors; D000324:Adrenocorticotropic Hormone; D013936:Thymidine; D006854:Hydrocortisone