Comparison of methods for determining DNase and phosphatase activities of staphylococci.
A greater percentage of DNase-positive strains was detected with DNase test agar than with DNase test agar containing 0.005% methyl green or 0.005% toluidine blue (P less than 0.01). No significant differences were obtained in the percentage of phosphatase-positive strains with the four methods compared. On the basis of ease of use, P agar containing para-nitrophenylphosphate disodium (0.495 mg/ml) would be the preferred method for determining phosphatase activity of staphylococci.
Langlois
B E
BE
Department of Animal Sciences, University of Kentucky, Lexington 40546-0215.
Harmon
R J
RJ
Akers
K
K
Aaron
D K
DK
D003470:Culture Media; D003851:Deoxyribonucleases / Q000378:metabolism*; D005189:False Positive Reactions; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D011933:Reagent Kits, Diagnostic; D013210:Staphylococcus / Q000201:enzymology*
D003470:Culture Media; D011933:Reagent Kits, Diagnostic; D003851:Deoxyribonucleases; D010744:Phosphoric Monoester Hydrolases