Purification and analysis of colonization factor antigen I, coli surface antigen 1, and coli surface antigen 3 fimbriae from enterotoxigenic Escherichia coli.
Enterotoxigenic Escherichia coli fimbriae are immunogenic and play a key role in intestinal colonization. Native colonization factor antigen I, coli surface antigen 1, and coli surface antigen 3 fimbriae were purified by a common method involving shearing, differential centrifugation, gel filtration, and density gradient ultracentrifugation. The compositions and N-terminal sequences were determined. Coli surface antigen 3 possesses two N-terminal isoforms, one of which matches the published DNA sequence, except for the previously proposed signal sequence cleavage point.
Hall
R H
RH
Department of Medicine, University of Maryland School of Medicine, Baltimore.
Maneval
D R
DR
Collins
J H
JH
Theibert
J L
JL
Levine
M M
MM
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000942:Antigens, Bacterial; D000954:Antigens, Surface / Q000032:analysis*; D001426:Bacterial Proteins / Q000302:isolation & purification; D004926:Escherichia coli / Q000032:analysis* / Q000472:pathogenicity; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000032:analysis*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012689:Sequence Homology, Nucleic Acid
D000596:Amino Acids; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001426:Bacterial Proteins; C022924:colonization factor antigens; D036781:Fimbriae Proteins