Changes in myocardial mitochondrial electron transport activity in rats administered with acetylcholinesterase inhibitor.
This study was designed to elucidate harmful effects of acetylcholine on myocardial mitochondrial electron transport activity. Rats were cervically dislocated 3 h and 6 h after oral administration of pyridostigmine, an acetylcholinesterase inhibitor. The myocardial mitochondrial electron-transport activity (NADH-cytochrome c reductase, succinate-cytochrome c reductase and cytochrome c oxidase), and myocardial acetylcholine and norepinephrine concentrations were measured. Activities of cytochrome c oxidase were significantly decreased in the pyridostigmine-3h and the pyridostigmine-6h groups compared with untreated rats. Activity of NADH-cytochrome c reductase was significantly decreased 6 h after administration. No significant changes were observed in those of succinate-cytochrome c reductase among all groups. Pyridostigmine increased significantly myocardial acetylcholine concentration, however, no significant changes of myocardial norepinephrine concentrations were observed among all groups. It is indicated that these mitochondrial injuries might be dependent on an increase in acetylcholine level and independent of norepinephrine.
Ito
T
T
Department of Internal Medicine, Faculty of Medicine, University of Nagoya, Japan.
Akiyama
N
N
Ogawa
T
T
Satake
T
T
Kato
T
T
Sugiyama
S
S
Ozawa
T
T
D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D004579:Electron Transport / Q000187:drug effects; D003576:Electron Transport Complex IV / Q000378:metabolism; D006321:Heart / Q000187:drug effects; D008297:Male; D008929:Mitochondria, Heart / Q000187:drug effects / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D009245:NADH Dehydrogenase / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism; D011729:Pyridostigmine Bromide / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D013384:Succinate Cytochrome c Oxidoreductase / Q000378:metabolism
D013384:Succinate Cytochrome c Oxidoreductase; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV; D011729:Pyridostigmine Bromide; D000109:Acetylcholine; D009638:Norepinephrine