Blockage of nerve growth factor action in PC12h cells by staurosporine, a potent protein kinase inhibitor.
Staurosporine, which has a structure similar to that of K-252a, a potent protein kinase inhibitor that blocks nerve growth factor (NGF) action in PC12 and PC12h cells, is also known as a potent inhibitor of several protein kinases. This study shows that in PC12h cells staurosporine has a dual action: at lower concentrations than that required by K-252a, it is an inhibitor of NGF induction of neurite formation and of changes in the phosphorylation of specific proteins, whereas at concentrations higher than that required to inhibit NGF-induced neurite outgrowth, it rapidly enhances outgrowth by itself.
Hashimoto
S
S
Department of Biochemistry, Ohu University School of Dentistry, Koriyama, Japan.
Hagino
A
A
D000310:Adrenal Gland Neoplasms; D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002460:Cell Line; D002474:Cell-Free System; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000302:isolation & purification / Q000378:metabolism; D009414:Nerve Growth Factors / Q000494:pharmacology*; D010673:Pheochromocytoma; D010710:Phosphates / Q000378:metabolism*; D010750:Phosphoproteins / Q000096:biosynthesis / Q000302:isolation & purification; D010766:Phosphorylation; D047428:Protein Kinase Inhibitors; D051381:Rats; D019311:Staurosporine; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
D000470:Alkaloids; D009363:Neoplasm Proteins; D009414:Nerve Growth Factors; D010710:Phosphates; D010750:Phosphoproteins; D047428:Protein Kinase Inhibitors; D003994:Bucladesine; D019311:Staurosporine