Laboratory diagnosis of Japanese encephalitis using monoclonal antibodies and correlation of findings with the outcome.
Detection of virus, viral antigen, and class-specific antibody was carried out in cerebrospinal fluid (CSF) and sera of 27 children with Japanese encephalitis. The diagnosis could be confirmed in 78.57% of cases (22/27) by demonstration of virus-specific IgM in CSF (15/22), viral antigen in CSF (5/22), or by virus isolation (2/22). Absence of virus specific IgM in CSF was associated with a fatal outcome (P = 0.05).
Ravi
V
V
Department of Neurovirology, National Institute of Mental Health and Neuro Sciences, Bangalore, India.
Vanajakshi
S
S
Gowda
A
A
Chandramuki
A
A
D000293:Adolescent; D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000134:cerebrospinal fluid; D000956:Antigens, Viral / Q000134:cerebrospinal fluid; D002648:Child; D002675:Child, Preschool; D004664:Encephalitis Virus, Japanese / Q000302:isolation & purification; D004672:Encephalitis, Japanese / Q000175:diagnosis*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D007223:Infant; D051379:Mice
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M