title: NMR studies of toxin III from the sea anemone Radianthus paumotensis and comparison of its secondary structure with related toxins.
abstract: Nearly complete assignments of the proton nuclear magnetic resonance (NMR) spectrum of the polypeptide toxin III from the sea anemone Radianthus paumotensis (RP) are presented. The secondary structures of the related toxins RP II and RP III are described and are compared with each other and with another related toxin ATX Ia from Anemonia sulcata [Widmer, H., Wagner, G., Schweitz, H., Lazdunski, M., & Wüthrich, K. (1988) Eur. J. Biochem. 171, 177-192]. All of these proteins contain a highly twisted four-strand antiparallel beta-sheet core connected by loops of irregular structure. From the work done with AP-A from Anthopleura xanthogrammica [Gooley, P. R., & Norton, R. S. (1986) Biochemistry 25, 2349-2356], it is clear that this homologous toxin also has the same basic core. Some small differences are seen in the structures of these toxins, particularly in the position of the N-terminal residues that form one of the outside strands of the beta-sheet. In addition, the R. paumotensis toxins are two residues longer, extending the third strand of sheet containing the C-terminal residues. A comparison of chemical shifts for assigned residues is also presented, in general supporting the similarity of structure among these proteins.
authors.affiliation: Department of Chemistry, University of California, Berkeley.
authors.forename: J H
authors.initials: JH
authors.lastname: Pease
authors.forename: N V
authors.initials: NV
authors.lastname: Kumar
authors.forename: H
authors.initials: H
authors.lastname: Schweitz
authors.forename: N R
authors.initials: NR
authors.lastname: Kallenbach
authors.forename: D E
authors.initials: DE
authors.lastname: Wemmer
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003064:Cnidarian Venoms; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D011487:Protein Conformation; D012615:Sea Anemones
chemical_list: D003064:Cnidarian Venoms; C032104:toxin III (Anemonia sulcata)