title: Effect of islet-activating protein (IAP) on glucagon release from isolated perifused rat islets.
abstract: The present study was undertaken to evaluate the effect of islet-activating protein (IAP) on glucagon release using perifused isolated rat pancreatic islets. Glucagon release stimulated by 20 mM arginine was significantly enhanced in the IAP-treated rat pancreatic islets as compared with the IAP-untreated controls. Additionally, the effect of 1 ng/ml somatostatin on glucagon release was examined during ongoing stimulation by arginine. In the IAP-treated islets somatostatin inhibits glucagon secretion to the same extent as in the IAP-untreated islets. These results demonstrate that IAP potentiates arginine-induced glucagon release from perifused rat pancreatic islets, and IAP fails to antagonize the inhibitory effect of somatostatin in the pancreatic A cell.
authors.forename: K
authors.initials: K
authors.lastname: Inoue
authors.forename: Y
authors.initials: Y
authors.lastname: Sako
authors.forename: A
authors.initials: A
authors.lastname: Hisatomi
authors.forename: F
authors.initials: F
authors.lastname: Umeda
authors.forename: H
authors.initials: H
authors.lastname: Nawata
mesh_terms: D000818:Animals; D001120:Arginine / Q000494:pharmacology; D005934:Glucagon / Q000378:metabolism*; D066298:In Vitro Techniques; D007515:Islets of Langerhans / Q000378:metabolism*; D008297:Male; D010477:Perfusion; D037342:Pertussis Toxin; D051381:Rats; D011919:Rats, Inbred Strains; D013004:Somatostatin / Q000494:pharmacology; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
chemical_list: D010566:Virulence Factors, Bordetella; D013004:Somatostatin; D005934:Glucagon; D001120:Arginine; D037342:Pertussis Toxin