An antiserum to the extracellular domain of the Alzheimer amyloid precursor recognizes 70 and 88 kDa brain proteins.
An antiserum raised to the extracellular domain (residues 556-566) of the Alzheimer amyloid precursor protein recognized 70 and 88 kDa proteins in Western blots of rat, Alzheimer, Down's syndrome and control human brain separated by SDS-PAGE. The 70 kDa protein band was resolved into 5 spots by two-dimensional electrophoresis. The findings provide further evidence that a 70 kDa protein is a metabolite of the amyloid precursor protein and reveal an 88 kDa protein which was reduced in 3 out of 6 brains with Alzheimer pathology.
Simpson
J
J
Department of Pharmacology, University of Edinburgh, Scotland.
Bladon
C M
CM
Yates
C M
CM
Harmar
A J
AJ
D000544:Alzheimer Disease / Q000378:metabolism; D000682:Amyloid / Q000276:immunology / Q000378:metabolism*; D016564:Amyloid beta-Protein Precursor; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism*; D004314:Down Syndrome / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005110:Extracellular Space / Q000378:metabolism; D006801:Humans; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000276:immunology* / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism; D011498:Protein Precursors / Q000276:immunology / Q000378:metabolism*
D000682:Amyloid; D016564:Amyloid beta-Protein Precursor; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; D011498:Protein Precursors