Rescue of Sendai virus from viral ribonucleoprotein-transfected cells by infection with recombinant vaccinia viruses carrying Sendai virus L and P/C genes.
The Sendai virus ribonucleoprotein (RNP) showed only very low plaque-forming titers upon transfection and the virus yields after one-step growth were quite limited. We tried to enhance the Sendai virus yield by supplying the viral L and P/C gene products through vaccinia vectors. A combination of the recombinant vaccinia viruses carrying the L gene (Vac-HL) and the P/C gene (Vac-HPC), both of which were driven by the promoter of the vaccinia virus 7.5K protein gene, enhanced the yield only a little whereas another combination of Vac-HLd7.5, the L gene insert of which was driven by the promoter of the vaccinia virus thymidine kinase gene in place of the 7.5K promoter, and Vac-HPC greatly enhanced the Sendai virus yield. This seemed to correlate with the fact that the Vac-HL interfered with Sendai virus growth markedly while the Vac-HLd7.5 did not. These results strongly suggest that the L and P/C gene products act in cooperation as the RNA polymerase, and overproduction of the L protein is inhibitory for Sendai virus growth. This system seems to be of value as a tool for analyzing the functions of L and P/C genes of Sendai virus.
Gotoh
H
H
Department of Viral Infection, The University of Tokyo, Japan.
Shioda
T
T
Sakai
Y
Y
Mizumoto
K
K
Shibuta
H
H
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002478:Cells, Cultured; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D004247:DNA / Q000235:genetics; D004274:DNA, Recombinant; D005455:Fluorescent Antibody Technique; D005814:Genes, Viral; D066298:In Vitro Techniques; D010222:Parainfluenza Virus 1, Human / Q000235:genetics* / Q000254:growth & development; D012261:Ribonucleoproteins / Q000235:genetics; D014616:Vaccinia virus / Q000235:genetics; D014779:Virus Replication
D004274:DNA, Recombinant; D012261:Ribonucleoproteins; D004247:DNA