Differential DNA binding of nuclear proteins to a long terminal repeat region of the MCF13 and Akv murine leukemia viruses.
Long terminal repeat (LTR) sequences of murine leukemia viruses (MLVs) have been demonstrated to be mainly responsible for the pathogenic differences in these retroviruses. A region of the LTR which is downstream of the enhancer elements has been shown to contribute both to enhancer activity as well as to disease specificity of MLVs. We have identified protein-DNA complexes generated by this region of a lymphomagenic MLV (MCF13) and one which is nonpathogenic (Akv). One protein-DNA complex we have observed for this region is unique to MCF13 DNA sequences. Detection of protein involved in this unique MCF13 complex in different cell lines revealed that it was ubiquitous.
Yoshimura
F K
FK
Department of Biological Structure, University of Washington, Seattle 98195.
Tupper
J
J
Diem
K
K
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism*; D004742:Enhancer Elements, Genetic; D009052:Leukemia Virus, Murine / Q000235:genetics*; D008223:Lymphoma; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity
D004279:DNA, Viral; D009687:Nuclear Proteins