Production and in vivo biologic actions of recombinant mouse interferon alpha 2.
We have expressed a recombinant mouse interferon alpha (r.Mu-IFN alpha 2) in Escherichia coli under the control of a tryptophan promoter using a synthetic adaptor formed by annealing two partially complementary oligonucleotides which introduced an ATG start codon and re-established the complete coding sequence of the mature IFN alpha 2 protein in the expression vector. Levels of up to 10(7) reference units of Mu-IFN alpha 2 per liter of culture were obtained using this construction. This recombinant mouse interferon alpha 2 exhibited antiviral activity in mice infected with EMC virus and antitumor activity in mice inoculated with Friend leukemia cells in a manner similar to that of natural mouse interferon alpha/beta.
Dron
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Laboratory of Viral Oncology, Centre National de la Recherche Scientifique, Villejuif.
Tovey
M G
MG
Maury
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C
Eid
P
P
Meyer
F
F
Gresser
I
I
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004686:Encephalomyocarditis virus / Q000187:drug effects / Q000502:physiology; D004926:Escherichia coli / Q000235:genetics; D005622:Friend murine leukemia virus / Q000187:drug effects / Q000502:physiology; D066298:In Vitro Techniques; D007370:Interferon Type I / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D007942:Leukemia, Experimental / Q000628:therapy; D051379:Mice; D008811:Mice, Inbred DBA; D008969:Molecular Sequence Data; D010957:Plasmids; D011994:Recombinant Proteins; D014779:Virus Replication / Q000187:drug effects
D007370:Interferon Type I; D011994:Recombinant Proteins; D004247:DNA