Dual-fluorescence flow cytometric analysis of membrane potential and cytoplasmic free Ca2+ concentration in embryonic rat hippocampal cells.
We have demonstrated simultaneous measurement of the membrane potential and cytoplasmic free Ca2+ concentration ([Ca2+]i) by utilizing a dual-laser flow cytometer in embryonic rat hippocampal cell suspensions. Veratrine, a Na+ channel activator, induced both membrane depolarization and elevation of [Ca2+]i. These actions of veratrine were all reversed by the presence of tetrodotoxin (TTX). These findings suggest that Na+ channels are functionally expressed in the cells and the activation of Na+ channels increases [Ca2+]i. The usefulness of the flow cytometric analysis in elucidating the expression of membrane functions in the embryonic central nervous systems (CNS) is discussed.
Mitsumoto
Y
Y
2nd Division, School of Pharmacy, Hokuriku University, Ishikawa, Japan.
Mohri
T
T
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D004622:Embryo, Mammalian / Q000378:metabolism; D005434:Flow Cytometry; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008564:Membrane Potentials; D051381:Rats; D015222:Sodium Channels / Q000187:drug effects / Q000378:metabolism; D013779:Tetrodotoxin / Q000494:pharmacology; D014702:Veratrine / Q000494:pharmacology
D015222:Sodium Channels; D013779:Tetrodotoxin; D014702:Veratrine; D002118:Calcium