A transcriptionally regulated expression vector for the fission yeast Schizosaccharomyces pombe.
An expression vector for the fission yeast Schizosaccharomyces pombe is described. The vector is designed to facilitate the construction of transcriptional fusions to the promoter of the S. pombe fructose bisphosphatase gene. Transcription from this promoter is regulated by glucose repression over a range of greater than 100-fold. The tight regulation by this promoter should allow for the maintenance of genes whose products are lethal to S. pombe and for the high level production of their protein or RNA products. Intermediate levels of expression can also be achieved by growth on different carbon sources.
Hoffman
C S
CS
Department of Genetics, Harvard Medical School, Boston, MA 02115.
Winston
F
F
D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D004271:DNA, Fungal; D006597:Fructose-Bisphosphatase / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005822:Genetic Vectors; D005947:Glucose / Q000378:metabolism; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D004718:Saccharomycetales / Q000235:genetics*; D012568:Schizosaccharomyces / Q000235:genetics*; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000235:genetics
D004271:DNA, Fungal; D006597:Fructose-Bisphosphatase; D001616:beta-Galactosidase; D005947:Glucose