Radiation-induced diploid spermatids in mice.
Diploid elongated spermatids of mice were enriched by flow cytometry and cell sorting using a new type of sorter (Partec). The sorted abnormal spermatids were identified morphologically and by nuclear area integration. The radiation-induced increase in the frequency of diploid elongated spermatids was monitored with time following acute X-ray exposure of mice. Dose-response curves for acute 60Co-gamma and 14 MeV neutron irradiations yielded an RBE value of 4.3 for the doubling of the control level.
Hacker-Klom
U
U
Institut für Strahlenbiologie, Universität, Münster, F.R. Germany.
Heiden
T
T
Otto
F J
FJ
Mauro
F
F
Göhde
W
W
D000818:Animals; D002469:Cell Separation; D003037:Cobalt Radioisotopes; D004171:Diploidy; D005214:Fast Neutrons; D005434:Flow Cytometry; D005720:Gamma Rays; D008297:Male; D051379:Mice; D012062:Relative Biological Effectiveness; D013087:Spermatids / Q000166:cytology / Q000528:radiation effects*
D003037:Cobalt Radioisotopes