Characterization of beta-adrenoceptor subtypes in rabbit mononuclear leukocytes.
Computer analysis of [125I]iodocyanopindolol competition studies using the relatively selective beta 1-adrenoceptor antagonist, ICI 89406, and the beta 2-selective antagonist, ICI 118551, on rabbit mononuclear leukocyte plasmalemmal preparations favored a two-site model indicating that both beta 1- and beta 2-adrenoceptor subtypes were present in approximately equal numbers. In contrast, similar studies performed on rabbit cardiac sarcolemma favored a one site model consistent with the presence of beta 1-adrenoceptors. Consequently, rabbit mononuclear leukocytes may provide a useful model for studying selective modulatory mechanisms of beta 1- and beta 2-adrenoceptors.
Tenner
T E
TE
Department of Pharmacology, Texas Tech University Health Sciences Center, Lubbock 79430.
Earley
K J
KJ
Young
J A
JA
D000818:Animals; D001667:Binding, Competitive; D066298:In Vitro Techniques; D020120:Iodocyanopindolol; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000378:metabolism; D010869:Pindolol / Q000031:analogs & derivatives / Q000097:blood; D011412:Propanolamines / Q000097:blood; D011817:Rabbits; D011943:Receptors, Adrenergic, beta / Q000032:analysis* / Q000145:classification
D011412:Propanolamines; D011943:Receptors, Adrenergic, beta; C026777:ICI 118551; D020120:Iodocyanopindolol; D010869:Pindolol; C025716:ICI 89406