Comparison of four charcoal media for the isolation of Bordetella pertussis.
Charcoal-horse blood agar with 40 micrograms of cephalexin per ml, charcoal-horse blood agar with 3 micrograms of lincomycin per ml, charcoal agar with 3 micrograms of lincomycin per ml, and Legionella (buffered charcoal-yeast extract) agar with 3 micrograms of lincomycin per ml were compared for isolation of Bordetella pertussis. Charcoal-horse blood agar with 40 micrograms of cephalexin per ml gave the best results, with a B. pertussis recovery rate of 100%. Growth was most rapid and the mean number of colonies was highest on this agar, and growth of pharyngeal flora was completely suppressed.
Hoppe
J E
JE
University Children's Hospital, Tübingen, Federal Republic of Germany.
Schwaderer
J
J
D000372:Agglutination Tests; D001886:Bordetella pertussis / Q000254:growth & development / Q000302:isolation & purification*; D002606:Charcoal; D015169:Colony Count, Microbial; D003470:Culture Media; D006801:Humans; D012463:Saliva / Q000382:microbiology
D003470:Culture Media; D002606:Charcoal