[Measurement of superoxide dismutase activity in normal skin by electron spin resonance-spin trapping method].
Superoxide dismutase (SOD) activity in 20 skin specimens from healthy individuals measured by the electron spin resonance-spin trapping method was 7.08 U +/- 0.41 U/mg protein (mean +/- SE). This method may be useful in the determination of SOD, since it requires a shorter time (approx. 3 min) and a smaller amount of specimen (approx. 30 mg) than previously reported methods other than the EIA method.
Yamagishi
R
R
Ichihara
T
T
Nagano
G
G
Kamide
R
R
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012867:Skin / Q000201:enzymology*; D013482:Superoxide Dismutase / Q000032:analysis*
D013482:Superoxide Dismutase