Cell mediated immune responses in ponies following infection with equine influenza virus (H3N8): the influence of induction culture conditions on the properties of cytotoxic effector cells.
Cytotoxic cell precursors and/or cytotoxic memory cells were demonstrated in the peripheral blood of ponies after aerosol infection with influenza A/equine/Newmarket/79 (H3N8). In order to reveal their cytotoxic potential, peripheral blood mononuclear cells required a secondary antigenic stimulation. In vitro induced cytotoxic cells showed activity against influenza infected target cells in a 3-4 h 51Cr-release assay. The reactivity of cytotoxic cells was markedly influenced by the conditions of the secondary induction culture. If high concentrations of exogenous crude equine IL-2 were used, virus infected target cells were susceptible to lysis by autologous or allogeneic effector cells. However, if IL-2 concentration was reduced, cytotoxic cells were generated which showed features consistent with cytotoxic T cells in that target-cell killing was genetically restricted.
Hannant
D
D
Department of Infectious Diseases, Animal Health Trust, Suffolk, Great Britain.
Mumford
J A
JA
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic / Q000662:veterinary; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D006736:Horses / Q000276:immunology*; D007111:Immunity, Cellular; D007156:Immunologic Memory; D053123:Influenza A Virus, H3N8 Subtype; D009980:Influenza A virus / Q000276:immunology; D007376:Interleukin-2 / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000662:veterinary*; D010835:Phytohemagglutinins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology
D000956:Antigens, Viral; D007376:Interleukin-2; D010835:Phytohemagglutinins