title: Immunocytochemical localization of the sex hormone-binding globulin in a human hepatoma cell line.
abstract: The human hepatoma cell line HEP G2 was investigated in an indirect immunofluorescence study for localization of the sex hormone-binding globulin. Cells were grown directly to confluency on the slides used for the immunocytochemical staining. A fine granular cytoplasmatic fluorescence pattern revealed the presence of sex hormone-binding globulin. Chang-liver cells used as controls, incubated under the same conditions, did not fluoresce. The synthesis of the hormone-binding protein in HEP G2 cells, which could be stimulated by L-thyroxine in the culture medium, was monitored by either indirect immunofluorescence or by immunoradiometric determination of sex hormone-binding globulin in the supernatant. These studies demonstrate that immunofluorescence techniques are able to detect sex hormone-binding globulin in human hepatoma cells.
authors.affiliation: Institute for Clinical Chemistry, Klinikum Grosshadern, University of Munich, FRG.
authors.forename: P
authors.initials: P
authors.lastname: Luppa
authors.forename: K
authors.initials: K
authors.lastname: Oettrich
authors.forename: I
authors.initials: I
authors.lastname: Schwab
authors.forename: U
authors.initials: U
authors.lastname: Langmandel
authors.forename: D
authors.initials: D
authors.lastname: Neumeier
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002461:Cell Line, Transformed; D005455:Fluorescent Antibody Technique; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism*; D012738:Sex Hormone-Binding Globulin / Q000378:metabolism*
chemical_list: D012738:Sex Hormone-Binding Globulin