title: Maturation-dependent changes of the rabbit reticulocyte energy metabolism.
abstract: Rabbit reticulocytes were separated into four fractions of different maturity in order to investigate the changes of cellular respiration and glycolysis, adenine nucleotides, 2,3-biphosphoglycerate (2,3-BPG) as well as cyclic AMP level during the transition from the youngest to the most mature reticulocytes. A significant reduction of total oxygen consumption, mainly due to depression of coupled respiration was found. The decline of respiration was accompanied by a 2-fold increase of the rate of aerobic glycolysis indicating a reduced Pasteur effect during maturation. A decline of ATP and an increase of ADP concentration was found. The oxygen-delivery capacity of the red cells increased by about 26% caused by an increase of the 2,3-BPG level of about 2 mmol/l cells. Cyclic AMP level in the fraction of youngest reticulocytes was about 60-fold higher than that in mature rabbit erythrocytes. The biggest decline of cyclic AMP was registered during the transition from youngest to the intermediate stage of maturity.
authors.affiliation: Institute of Physiology, Faculty of Medicine, University S. Markovic, Kragujevac, Yugoslavia.
authors.forename: M M
authors.initials: MM
authors.lastname: Kostić
authors.forename: S M
authors.initials: SM
authors.lastname: Rapoport
mesh_terms: D019794:2,3-Diphosphoglycerate; D000227:Adenine Nucleotides / Q000097:blood*; D000244:Adenosine Diphosphate / Q000097:blood; D000255:Adenosine Triphosphate / Q000097:blood; D000818:Animals; D000242:Cyclic AMP / Q000097:blood; D004163:Diphosphoglyceric Acids / Q000097:blood; D004734:Energy Metabolism; D004905:Erythrocyte Aging; D006019:Glycolysis; D010101:Oxygen Consumption; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism*
chemical_list: D000227:Adenine Nucleotides; D004163:Diphosphoglyceric Acids; D019794:2,3-Diphosphoglycerate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D000242:Cyclic AMP