A single point mutation confers tetrodotoxin and saxitoxin insensitivity on the sodium channel II.
A single point mutation of the rat sodium channel II reduces its sensitivity to tetrodotoxin and saxitoxin by more than three orders of magnitude. The mutation replaces glutamic acid 387 with a glutamine and has only slight effects on the macroscopic current properties, as measured under voltage-clamp in Xenopus oocytes injected with the corresponding cDNA-derived mRNA.
Noda
M
M
Department of Medical Chemistry, Kyoto University Faculty of Medicine, Japan.
Suzuki
H
H
Numa
S
S
Stühmer
W
W
D000595:Amino Acid Sequence; D000818:Animals; D004553:Electric Conductivity; D008564:Membrane Potentials; D009154:Mutation; D051381:Rats; D012530:Saxitoxin / Q000494:pharmacology*; D015222:Sodium Channels / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D013329:Structure-Activity Relationship; D013779:Tetrodotoxin / Q000494:pharmacology*; D014982:Xenopus laevis
D015222:Sodium Channels; D012530:Saxitoxin; D013779:Tetrodotoxin