Episomal HPV 16 DNA isolated from a cervical carcinoma presents a partial duplication of the early region.
An invasive cervical carcinoma was found to harbor an episomal variant of human papillomavirus (HPV) type 16 DNA, with a size of about 10.1 kb. A genomic library of the tumor was constructed in bacteriophage lambda and a recombinant phage clone was isolated by screening with HPV 16 probe. Analysis by restriction mapping and Southern hybridization showed that the isolate contained a 2.2 kb duplication of the early region, which included part of E6, all E7 and part of E1 open reading frames. Possible consequences of this duplication for oncogenesis are discussed.
Di Luca
D
D
Istituto di Microbiologia, Università di Ferrara, Italy.
Caselli
E
E
Monini
P
P
Rotola
A
A
Savioli
A
A
Cassai
E
E
D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D015321:Gene Rearrangement; D005814:Genes, Viral; D006801:Humans; D005810:Multigene Family; D027383:Papillomaviridae / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D015183:Restriction Mapping; D002583:Uterine Cervical Neoplasms / Q000382:microbiology*
D015344:DNA Probes, HPV; D004279:DNA, Viral