Isolation of nit-4, the minor nitrogen regulatory gene which mediates nitrate induction in Neurospora crassa.
Expression of nitrate reductase in Neurospora crassa requires the positive action of nit-4, a pathway-specific regulatory gene, which mediates nitrate induction. We report the molecular cloning of the nit-4 gene and present results which suggest that the nit-4 gene is constitutively expressed to yield a low-abundance 2.2-kilobase transcript. These results indicate that the nit-2 major control gene and the nit-4 pathway-specific control gene independently regulate the expression of the nitrate assimilatory structural genes.
Fu
Y H
YH
Department of Biochemistry, Ohio State University, Columbus 43210.
Kneesi
J Y
JY
Marzluf
G A
GA
D003001:Cloning, Molecular; D004790:Enzyme Induction; D005786:Gene Expression Regulation; D005800:Genes, Fungal; D009491:Neurospora / Q000235:genetics*; D009492:Neurospora crassa / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000096:biosynthesis; D009566:Nitrates / Q000502:physiology*; D009572:Nitrite Reductases / Q000096:biosynthesis; D009586:Nitrogen Fixation / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length
D009566:Nitrates; D009565:Nitrate Reductases; D009572:Nitrite Reductases; D050901:Nitrate Reductase