Nucleotide sequence of the Rickettsia prowazekii ATP/ADP translocase-encoding gene.
The Rickettsia prowazekii ATP/ADP translocase (Tlc) gene (tlc), previously cloned in Escherichia coli was localized to a 1.6-kb chromosomal fragment. Nucleotide sequence analysis of this fragment revealed an open reading frame of 1494 bp that could encode a hydrophobic protein of 497 amino acids (aa) with an Mr of 56,668. Analysis of the deduced aa sequence revealed that it contained twelve potential membrane-spanning regions. Comparisons between the deduced aa sequence of the R. prowazekii ATP/ADP Tlc and the sequences of mitochondrial (mt) Tlc revealed no detectable homologies between the rickettsial and mt sequences. The major protein synthesized in E. coli minicells containing the rickettsial gene exhibited and Mr of approx. 34,000.
Williamson
L R
LR
Department of Microbiology and Immunology, University of South Alabama College of Medicine, Mobile 36688.
Plano
G V
GV
Winkler
H H
HH
Krause
D C
DC
Wood
D O
DO
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D000226:Mitochondrial ADP, ATP Translocases / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D009713:Nucleotidyltransferases / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis; D012283:Rickettsia prowazekii / Q000235:genetics*
D003062:Codon; D011994:Recombinant Proteins; D000226:Mitochondrial ADP, ATP Translocases; D009713:Nucleotidyltransferases