A new in vitro screening system for anticancer drugs for the treatment of non-small cell lung cancer.
We have evaluated a semiautomated radiometric assay (BACTEC 460 system) for screening of activity of anticancer drugs against human non-small cell lung cancer cell lines. Cells from seven cell lines were exposed to standard antineoplastic agents at four different concentrations using a 1-h incubation. Alpha 2-interferon was tested using a continuous incubation. In vitro drug activity was analyzed as a function of the clinically achievable serum concentration. Our results indicate that two cell lines (CALU-3, SK-MES-1) exhibit in vitro drug sensitivity patterns closest to those observed in clinical studies. These two cell lines might therefore be most useful for screening new anticancer compounds for activity against non-small cell lung cancer. The radiometric assay is a semiautomated system which has advantages over other, more time-consuming screening systems.
Hanauske
U
U
Department of Medicine, University of Texas Health Science Center, San Antonio.
Hanauske
A R
AR
Clark
G M
GM
Tsen
D
D
Buchok
J
J
von Hoff
D D
DD
D000970:Antineoplastic Agents / Q000627:therapeutic use*; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy*; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D005069:Evaluation Studies as Topic; D006128:Growth; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy*; D011874:Radiometry / Q000379:methods; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000502:physiology
D000970:Antineoplastic Agents