[Analysis of the effect of quisqualate, N-methyl-D- aspartate and several blockers of amino acid receptors on synaptic transmission in the ampullae of Lorenzini].
Effects of bath-application of quisqualate (Q), N-methyl-D-aspartate (NMDA) and antagonists of NMDA-receptors: D-amino-adipate (AA), 2-amino-4-phosphonobutyrate (APB), 2-amino-5-phosphonovalerate (APV) and Mg2+ as well as acidic amino acid antagonist: D-glutamylglycine (DGG) on the synapse between the electroreceptor cells and afferent fibres were studied in the ampullae of Lorenzini. Q (threshold concentration 10(-8) M) and NMDA (threshold concentration 10(-5) M) strongly excited afferent fibres. Neither AA nor APB influenced the resting and evoked activities. APV blocked the synaptic transmission. Mg2+ (30-50 mM) blocked the responses to NMDA, while Q-induced responses were not affected, APV preferentially blocked NMDA-induced responses and in lesser degree--L-aspartate (L-ASP)-induced responses. DGG blocked the synaptic transmission. It is supposed that the synaptic receptor could represent a homogeneous receptor with different binding sites to the known agonists.
Akoev
G N
GN
Andrianov
Iu N
IuN
Bromm
B
B
Sabo
T
T
Sherman
N O
NO
D015763:2-Amino-5-phosphonovalerate; D015074:2-Aminoadipic Acid / Q000494:pharmacology; D000613:Aminobutyrates / Q000494:pharmacology; D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D004555:Electric Fish / Q000502:physiology*; D005071:Evoked Potentials / Q000187:drug effects; D008274:Magnesium / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects; D016202:N-Methylaspartate; D010069:Oxadiazoles / Q000494:pharmacology*; D016318:Quisqualic Acid; D016194:Receptors, N-Methyl-D-Aspartate; D017981:Receptors, Neurotransmitter / Q000187:drug effects; D011984:Sensory Receptor Cells / Q000187:drug effects / Q000502:physiology*; D012860:Skates, Fish / Q000502:physiology*; D013569:Synapses / Q000187:drug effects* / Q000502:physiology; D009435:Synaptic Transmission / Q000187:drug effects; D014633:Valine / Q000031:analogs & derivatives / Q000494:pharmacology
D000613:Aminobutyrates; D010069:Oxadiazoles; D016194:Receptors, N-Methyl-D-Aspartate; D017981:Receptors, Neurotransmitter; D015074:2-Aminoadipic Acid; D001224:Aspartic Acid; D016202:N-Methylaspartate; D015763:2-Amino-5-phosphonovalerate; D016318:Quisqualic Acid; C012729:2-amino-4-phosphonobutyric acid; D014633:Valine; D008274:Magnesium
Analiz vliiania kviskvalata, N-metil-D-aspartata i nekotorykh blokatorov retseptorov aminokislot na sinapticheskuiu peredachu v ampulakh Lorentsini.