Adenylate cyclase activity in homogenates of human melanoma cells. Effect of alpha-MSH and isoprenaline.
The effects of the alpha-melanocyte-stimulating hormone (alpha-MSH) (10(-7)-10(-5) M) and the beta-adrenoceptor agonist isoprenaline (10(-9)-10(-4) M) on adenylate cyclase (AC) activity were investigated in homogenates of the human IGR 1 melanoma cells with or without additional GTP. Basal AC activity was increased by the administration of 10 microM GTP. Alpha-MSH had no effect on cyclic AMP (cAMP) accumulation, while isoprenaline stimulated AC activity in a dose-dependent manner.
Karg
E
E
Department of Dermatology, University of Lund, Sweden.
Johansson
L H
LH
Hindemith-Augustsson
A
A
Rosengren
E
E
Rorsman
H
H
D000262:Adenylyl Cyclases / Q000378:metabolism*; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000032:analysis; D006160:Guanosine Triphosphate / Q000494:pharmacology; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology*; D009074:Melanocyte-Stimulating Hormones / Q000494:pharmacology*; D008545:Melanoma / Q000201:enzymology*
D006160:Guanosine Triphosphate; D009074:Melanocyte-Stimulating Hormones; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D007545:Isoproterenol