Distribution and metabolism of leukotriene C4 after cisternal injection in guinea pigs.
Following cisternal injection of [3H8]LTC4 into guinea pigs, leukotriene metabolites were identified in the brain, cerebellum, perilymph, blood, liver and kidneys. LTC4 was metabolized into LTD4 and LTE4 in the cerebrospinal fluid and LTE4 was transported into the blood for general circulation and uptake into the liver and kidneys. The excretion of LTE4 from CNS to blood seemed to be the rate-limiting step in the elimination of leukotrienes from the body. Leukotrienes were also transported into the perilymph. The conversion of LTC4 into LTD4 and LTE4 was lower in perilymph as compared to the cerebrospinal fluid, suggesting a rate limiting function of the cochlear aqueduct that can be defined as a cerebrospinal fluid-labyrinth barrier.
Juhn
S K
SK
Department of Physiological Chemistry, Karolinska Institute, Sweden.
Orning
L
L
Hammarström
S
S
Hedqvist
P
P
Wersäll
J
J
D000818:Animals; D001711:Biotransformation; D001921:Brain / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002946:Cisterna Magna; D006168:Guinea Pigs; D007267:Injections; D017999:Leukotriene E4; D013189:SRS-A / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000493:pharmacokinetics*; D014018:Tissue Distribution
D013189:SRS-A; D017999:Leukotriene E4