title: Cyclin is a component of the sea urchin egg M-phase specific histone H1 kinase.
abstract: A so-called 'growth-associated' or 'M-phase specific' histone H1 kinase (H1K) has been described in a wide variety of eukaryotic cell types; p34cdc2 has previously been shown to be a catalytic subunit of this protein kinase. In fertilized sea urchin eggs the activity of H1K oscillates during the cell division cycle and there is a striking temporal correlation between H1K activation and the accumulation of a phosphorylated form of cyclin. H1K activity declines in parallel with proteolytic cyclin destruction of the end of the first cell cycle. By virtue of the high affinity of the fission yeast p13suc1 for the p34cdc2 protein, H1K strongly binds to p13-Sepharose beads. Cyclin, p34cdc2 and H1K co-purify on this affinity reagent as well as through several conventional chromatographic procedures. Anticyclin antibodies immunoprecipitate the M-phase specific H1K in crude extracts or in purified fractions. Sea urchin eggs appear to contain much less cyclin than p34cdc2, suggesting that p34cdc2 may interact with other proteins. These results demonstrate that cyclin and p34cdc2 are major components of the M-phase specific H1K.
authors.affiliation: CNRS, Station Biologique, Roscoff, France.
authors.forename: L
authors.initials: L
authors.lastname: Meijer
authors.forename: D
authors.initials: D
authors.lastname: Arion
authors.forename: R
authors.initials: R
authors.lastname: Golsteyn
authors.forename: J
authors.initials: J
authors.lastname: Pines
authors.forename: L
authors.initials: L
authors.lastname: Brizuela
authors.forename: T
authors.initials: T
authors.lastname: Hunt
authors.forename: D
authors.initials: D
authors.lastname: Beach
mesh_terms: D000818:Animals; D016203:CDC2 Protein Kinase; D002846:Chromatography, Affinity; D016213:Cyclins; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D005260:Female; D005306:Fertilization; D007447:Invertebrate Hormones / Q000378:metabolism*; D008297:Male; D008540:Meiosis; D008677:Metaphase; D008938:Mitosis; D010063:Ovum / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D006656:Protamine Kinase / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism*; D012617:Sea Urchins; D013997:Time Factors
chemical_list: D016213:Cyclins; D007447:Invertebrate Hormones; D010750:Phosphoproteins; D011494:Protein Kinases; D006656:Protamine Kinase; D016203:CDC2 Protein Kinase