False-positive reactions associated with anti-mouse activity in serotests for feline leukemia virus antigen.
False-positive reactions were observed in commercial test kits designed to detect FeLV infections in cats. The false-positive reactions were associated with heterophilic antibody in cat serum directed primarily against mouse immunoglobulins. Of 2,830 sera tested, 579 were considered FeLV positive or equivocal via ELISA; of these, 10 were found that did not have FeLV antigen but did have anti-mouse antibody. With 95% confidence, 0.14% to 0.57% of cats would have such false-positive reactions in ELISA, because one in 283 (0.35%) cats had anti-mouse antibodies.
Lopez
N A
NA
Veterinary Diagnostic Laboratory, New York State College of Veterinary Medicine, Cornell University, Ithaca, NY 14853.
Jacobson
R H
RH
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis*; D002371:Cat Diseases / Q000175:diagnosis*; D002415:Cats; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005189:False Positive Reactions; D006454:Hemoglobins / Q000032:analysis; D006461:Hemolysis; D007136:Immunoglobulins / Q000276:immunology; D007938:Leukemia / Q000175:diagnosis / Q000662:veterinary*; D005256:Leukemia Virus, Feline / Q000276:immunology*; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic / Q000662:veterinary
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D006454:Hemoglobins; D007136:Immunoglobulins; D011933:Reagent Kits, Diagnostic