A comparison of norepinephrine- and ACTH-stimulated lipolysis in white and brown adipocytes of female rats.
1. Isolated white and brown adipocytes (WFA and BFA) from the rat were compared with respect to their lipolytic responsiveness towards norepinephrine (NE) and adrenocorticotrophic hormone (ACTH). 2. NE yielded a Km value of 702.7 +/- 30.6 nM for WFA and 142.5 +/- 7.2 nM for BFA. The maximum lipolytic response (Vm) was 145.7 +/- 1.2 nmol glycerol/micrograms DNA/90 min for WFA and 23.7 +/- 0.2 nmol glycerol/micrograms DNA/90 min for BFA. 3. ACTH yield Km values of 31.6 +/- 1.5 and 31.9 +/- 3.1 nM for WFA and BFA, respectively. Vm values of 141.9 +/- 1.0 and 34.2 +/- 0.5 nmol glycerol/micrograms DNA/90 min were observed for WFA and BFA, respectively.
Van Heerden
M
M
Department of Biochemistry, University of Port Elizabeth, Republic of South Africa.
Oelofsen
W
W
D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D002001:Adipose Tissue, Brown / Q000166:cytology / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000502:physiology*; D000818:Animals; D002021:Buffers; D002452:Cell Count; D005260:Female; D005990:Glycerol / Q000378:metabolism; D066298:In Vitro Techniques; D008066:Lipolysis / Q000502:physiology*; D009638:Norepinephrine / Q000502:physiology*; D051381:Rats; D013696:Temperature
D002021:Buffers; D000324:Adrenocorticotropic Hormone; D005990:Glycerol; D009638:Norepinephrine