Chronic intrastriatal L-pyroglutamate: neuropathology and neuron sparing like Huntington's disease.
Continuous injection of L-pyroglutamate (L-PGA) into the rat striatum induces a lesion with three regions: a necrotic core, a rim of pyknotic cells, and a peripheral spongiose region. The L-PGA was administered through an implanted intrastriatal cannula coupled to an Alzet osmotic pump loaded with one of three doses of L-PGA (3, 5, or 13 times the normal amount of L-PGA/g wet wt rat forebrain (23 nmol/g). The magnitude of the lesion was dependent upon the concentration of buffered L-PGA and the duration of continuous pumping. The necrotic region contained macrophages and neutrophils, while condensed neurons and oligodendroglial cells were present in the pyknotic region. The spongiose region contained vacuolated neuropil and degenerating nerve cells and oligodendroglia. The spongiose region blends with the normal neuropil. A population of aspiny neurons were identified throughout the spongiose region. These neurons stained positive for NADPH diaphorase and demonstrated a somatostatin-like immunoreactivity similar to that of the aspiny neurons spared in Huntington's disease and in the neurotoxin-induced striatal-lesioned rat models of Huntington's disease.
Rieke
G K
GK
Department of Anatomical Sciences, Meharry Medical College, Nashville, Tennessee 37208.
Smith
J
J
Idusuyi
O B
OB
Semenya
J
J
Howard
R
R
Williams
S
S
D000818:Animals; D003342:Corpus Striatum / Q000187:drug effects; D006816:Huntington Disease / Q000473:pathology*; D008297:Male; D009252:NADPH Dehydrogenase / Q000032:analysis; D009474:Neurons / Q000187:drug effects / Q000473:pathology*; D009498:Neurotoxins / Q000633:toxicity; D011760:Pyrrolidinones / Q000633:toxicity*; D011761:Pyrrolidonecarboxylic Acid / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains; D013004:Somatostatin / Q000032:analysis
D009498:Neurotoxins; D011760:Pyrrolidinones; D013004:Somatostatin; D009252:NADPH Dehydrogenase; D011761:Pyrrolidonecarboxylic Acid