Synergism between chemotactic peptide and platelet-activating factor in stimulating thromboxane B2 and leukotriene B4 biosynthesis in human neutrophils.
Formyl-Met-Leu-Phe (FMLP) and platelet-activating factor (PAF) were capable of stimulating thromboxane B2 (TXB2) and leukotriene B4 (LTB4) syntheses in human neutrophils, albeit in a relatively poor degree. A combination of FMLP and PAF, however, was synergistic in stimulating TXB2 and LTB4 syntheses. Phorbol myristate acetate (PMA) appeared to attenuate PAF- but not FMLP-induced arachidonate metabolism. These results suggest that cooperative action of FMLP and PAF on arachidonate release and metabolism does exist and that PMA-mediated protein kinase C activation may regulate FMLP and PAF actions in a different manner.
Tanizawa
H
H
Division of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Kentucky, Lexington 40536-0082.
Tai
H H
HH
D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000097:blood; D006801:Humans; D066298:In Vitro Techniques; D007975:Leukotriene B4 / Q000096:biosynthesis*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000502:physiology*; D009504:Neutrophils / Q000378:metabolism*; D010972:Platelet Activating Factor / Q000502:physiology*; D013929:Thromboxane B2 / Q000096:biosynthesis*
D001095:Arachidonic Acids; D010972:Platelet Activating Factor; D007975:Leukotriene B4; D016718:Arachidonic Acid; D013929:Thromboxane B2; D009240:N-Formylmethionine Leucyl-Phenylalanine