Immobilized GABAA receptors and their ligand binding characteristics.
ConA-sepharose and polylysine-agarose beads effectively bound detergent-solubilized GABAA receptors from rat cerebrocortical membranes. The immobilized receptors showed a single class of high affinity binding sites specific for flunitrazepam or muscimol and displayed GABA-stimulated flunitrazepam binding. Maximal binding capacities of the ConA-immobilized receptor for the ligands were about three times greater than those of the polylysine-immobilized receptors. The relative affinities for each of the ligands were not affected by the method of receptor immobilization. The dissociation constants for muscimol of these immobilized receptors were somewhat dependent on the solubilizing agents used, but were considerably lower than those measured using extensively dialyzed rat cerebrocortical membranes.
Im
W B
WB
CNS Research Upjohn Company Kalamazoo, MI 49001.
Tai
M M
MM
Blakeman
D P
DP
Davis
J P
JP
D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism; D003208:Concanavalin A / Q000378:metabolism; D005445:Flunitrazepam / Q000378:metabolism; D066298:In Vitro Techniques; D008024:Ligands; D009118:Muscimol / Q000378:metabolism; D011107:Polylysine / Q000378:metabolism; D051381:Rats; D011963:Receptors, GABA-A / Q000378:metabolism*; D012995:Solubility; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D008024:Ligands; D011963:Receptors, GABA-A; D003208:Concanavalin A; D011107:Polylysine; D009118:Muscimol; D005680:gamma-Aminobutyric Acid; D005445:Flunitrazepam