Association of Reoviridae particles in an enteric syndrome of poults observed in turkey flocks during 1988.
An enteric syndrome of turkey poults, characterized by enteritis, crop mycosis, intestinal changes (pale, thin-walled ballooning with watery contents), and rickets, occurred during 1988 in 74 turkey flocks from different farms belonging to 9 California turkey growers. The flocks ranged in size from 9,000 to 120,000 birds. Pools of intestine sections from 618 birds, representing 78 field cases, were examined. Histopathological examination of the intestines showed a mild to severe atrophy with a reduced depth of crypts, which was more prominent in the distal part of the small intestine. Viral isolation attempts with primary cell cultures of chicken embryo kidney cells were negative. Examination by electron microscopy of negatively stained intestinal specimens revealed the presence of Reoviridae particles of 58.8 to 80 nm in diameter. Enzyme-linked immunosorbent assay results on the intestinal pools for mammalian and group A avian rotaviruses were negative. A statistically significant relationship was found for the presence of Reoviridae particles in the intestines of 10-21-day-old birds. Of the 7 most common pathological conditions analyzed, 2, rickets and intestinal changes (thin-walled ballooning intestine with watery contents), showed a statistically significant association with the presence of Reoviridae particles.
Lozano
L F
LF
California Veterinary Diagnostic Laboratory System, University of California-Davis 95616.
Bickford
A A
AA
Castro
A E
AE
Swartzman-Andert
J
J
Chin
R
R
Meteyer
C
C
Cooper
G
G
Reynolds
B
B
Manalac
R L
RL
D000367:Age Factors; D000818:Animals; D003967:Diarrhea / Q000382:microbiology / Q000473:pathology / Q000662:veterinary; D004751:Enteritis / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D007422:Intestines / Q000473:pathology; D008854:Microscopy, Electron; D011201:Poultry Diseases / Q000382:microbiology* / Q000473:pathology; D012087:Reoviridae / Q000302:isolation & purification* / Q000648:ultrastructure; D012088:Reoviridae Infections / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D012475:Salmonella / Q000302:isolation & purification; D013577:Syndrome; D014422:Turkeys; D014771:Virion / Q000302:isolation & purification / Q000648:ultrastructure