Interferon-mediated inhibition of retroviral infection: use of a defective retrovirus carrying a drug-resistance gene.
We here report the results of an investigation of the effect of interferon on the establishment of new infections by a retrovirus. For this study, we used an infectious but replication-incompetent retrovirus carrying a drug-resistance gene and assayed for infectivity by measuring drug-resistant colony formation. Mouse interferon-beta inhibited retroviral infection of mouse CG1 cells in a dose-dependent manner. However, a higher dose of interferon was needed for eliciting the antiretroviral effects than for action against vesicular stomatitis virus. The degree of antiretroviral effect was comparable over at least a 100-fold range of multiplicity of infection and the effect was most pronounced when the cells were continuously treated with interferon before infections and during infection and drug-selection.
Kumar
R
R
Memorial Sloan-Kettering Cancer Center, New York, NY 10021.
Sen
G C
GC
D000818:Animals; D002460:Cell Line; D003673:Defective Viruses / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D004352:Drug Resistance, Microbial / Q000235:genetics; D007370:Interferon Type I / Q000494:pharmacology*; D008242:Lysogeny / Q000187:drug effects; D012190:Retroviridae / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D012192:Retroviridae Infections / Q000382:microbiology / Q000517:prevention & control*; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects / Q000502:physiology; D014779:Virus Replication / Q000187:drug effects
D007370:Interferon Type I