Effect of storage and 60Co irradiation on the antiviral activity and subtype pattern of native human interferon-alpha preparations.
The stability of native human leukocyte IFN-alpha was investigated after one year storage at different temperatures, or after sterilization with 60Co-irradiation by determining the total antiviral activity of samples. Inactivation of VSV and Aujeszky virions by 60Co-irradiation was directly related to the radiolysis of samples, indicating the uselessness of this procedure for sterilization of IFN-alpha preparations. The presence and proportion of subtypes in the stored or irradiated preparations (with 50 and 25-70% inactivation, respectively) was analysed by chromatofocusing, comparing their patterns with that of the untreated controls. A logarithmic correlation was found between the pI values and temperature/irradiation sensitivity of subtypes.
Mécs
I
I
Institute of Microbiology, A. Szent-Györgyi University of Medicine, Szeged, Hungary.
Tóth
S
S
Sirokmán
F
F
D000998:Antiviral Agents / Q000494:pharmacology / Q000528:radiation effects*; D003037:Cobalt Radioisotopes; D004355:Drug Stability; D004356:Drug Storage; D005720:Gamma Rays; D007370:Interferon Type I / Q000145:classification / Q000494:pharmacology / Q000528:radiation effects*; D007526:Isoelectric Point; D013242:Sterilization / Q000379:methods*; D013696:Temperature; D014780:Viruses / Q000528:radiation effects
D000998:Antiviral Agents; D003037:Cobalt Radioisotopes; D007370:Interferon Type I