A liver-specific nuclear protein that binds to the distal promoter element of the rat tyrosine aminotransferase gene.
Using a gel retardation assay and exonuclease III footprinting, we have analyzed sequence-specific DNA-binding nuclear factors which interact with the distal promoter element of the rat tyrosine aminotransferase gene. A factor called LspA1, binding to a sequence that resembles the consensus binding site for the transcription factor Ap-1, was shown to be present in adult rat-liver nuclear protein extracts but not in the extracts from embryonic liver or spleen nuclei.
Lobanenkov
V V
VV
Institute of Carcinogenesis, All-Union Cancer Research Center, Moscow, USSR.
Klenowa
E M
EM
Adler
V V
VV
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000378:metabolism; D005090:Exodeoxyribonucleases; D005782:Gels; D008099:Liver / Q000032:analysis* / Q000196:embryology; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D051381:Rats; D015183:Restriction Mapping; D014444:Tyrosine Transaminase / Q000235:genetics*
D004268:DNA-Binding Proteins; D005782:Gels; D009687:Nuclear Proteins; D014444:Tyrosine Transaminase; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III