Transient shut off of Escherichia coli heat shock protein synthesis upon temperature shift down.
A moderate downward shift in growth temperature (37 to 30 degrees C in strain B/r and 37 to 24 degrees C in strain K-12) was found to depress markedly the synthesis of major heat shock proteins GroEL and DnaK in E. coli. The depression was transient and cancelled gradually to a new steady state level, taking 60-80 min. The synthesis of beta-galactosidase directed by transcription initiated at the groE promoter behaved similarly, suggesting that this regulation, termed "reverse heat shock response", occurs at the transcriptional level.
Taura
T
T
Institute for Virus Research, Kyoto University, Japan.
Kusukawa
N
N
Yura
T
T
Ito
K
K
D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D018834:Chaperonin 60; D018833:Chaperonins; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005786:Gene Expression Regulation; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D007526:Isoelectric Point; D008970:Molecular Weight; D013696:Temperature; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D018834:Chaperonin 60; D029968:Escherichia coli Proteins; C028715:GroE protein, E coli; D006360:Heat-Shock Proteins; D018833:Chaperonins