title: [Immobilized oligonucleotides as affinity sorbents for restriction endonucleases].
abstract: Affinity chromatography of IIS type restriction endonucleases is proposed. It is shown that endonucleases HgaI, FokI, and SfaNI have affinity to the matrix with immobilized oligonucleotides which contain the endonuclease's recognition sites resistant to the hydrolysis.
authors.forename: S Kh
authors.initials: SKh
authors.lastname: Degtiarev
authors.forename: P A
authors.initials: PA
authors.lastname: Belavin
authors.forename: I G
authors.initials: IG
authors.lastname: Shishkina
authors.forename: V F
authors.initials: VF
authors.lastname: Zarytova
authors.forename: L P
authors.initials: LP
authors.lastname: Gavriuchenkova
mesh_terms: D001483:Base Sequence; D002846:Chromatography, Affinity; D004262:DNA Restriction Enzymes; D004587:Electrophoresis, Agar Gel; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009841:Oligonucleotides
chemical_list: D009841:Oligonucleotides; D004262:DNA Restriction Enzymes
vernacular_title: Immobilizovannye oligonukleotidy kak affinnye sorbenty dlia éndonukleaz restriktsii.