title: Antigen presentation by B cells and macrophages of cytochrome c and its antigenic fragment when conjugated to the surface of liposomes.
abstract: An in vitro antigen presentation system was used to study how antigens coupled to the surface of phospholipid vesicles (liposomes) are presented to antigen specific T cells. Liposome-bound pigeon cytochrome c (PCC) was 30-40-fold more potent than free PCC when peritoneal macrophages were the presenting cell. B cells presented surface-bound PCC, albeit less efficiently than unmodified PCC. Surface-bound peptide epitope was presented by both cell types, but not as efficiently as unmodified peptide. With the T cell epitope, antigen processing was not required since glutaraldehyde fixed cells could present surface-bound peptide.
authors.affiliation: Department of Pharmacy and Pharmaceutical Chemistry, School of Pharmacy, University of California, San Francisco 94143-0446.
authors.forename: P R
authors.initials: PR
authors.lastname: Dal Monte
authors.forename: F C
authors.initials: FC
authors.lastname: Szoka
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D010856:Columbidae; D003574:Cytochrome c Group / Q000276:immunology*; D006825:Hybridomas; D007376:Interleukin-2 / Q000096:biosynthesis; D008081:Liposomes / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D006377:T-Lymphocytes, Helper-Inducer / Q000378:metabolism
chemical_list: D000276:Adjuvants, Immunologic; D003574:Cytochrome c Group; D007376:Interleukin-2; D008081:Liposomes