title: Effects of calcitonin gene-related peptide on airway epithelial functions in dogs.
abstract: We studied the effects of calcitonin gene-related peptide (CGRP) on ciliary beat frequency (CBF) and electrical properties of canine tracheal epithelium by a photoelectric method and Ussing's short-circuit technique, respectively. CGRP dose dependently increased CBF, an effect that was accompanied by elevation of intracellular cyclic AMP but not affected by blockade of either Ca2+-influx or arachidonic acid metabolism. In contrast, CGRP elicited only a small and transient increase in short-circuit current without significant alterations in transepithelial potential difference or tissue conductance. These results suggest that CGRP may play a role in regulating airway mucociliary transport function.
authors.affiliation: First Department of Medicine, Tokyo Women's Medical College, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Tamaoki
authors.forename: T
authors.initials: T
authors.lastname: Kanemura
authors.forename: K
authors.initials: K
authors.lastname: Kobayashi
authors.forename: N
authors.initials: N
authors.lastname: Sakai
authors.forename: T
authors.initials: T
authors.lastname: Takizawa
mesh_terms: D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000502:physiology*; D002923:Cilia / Q000502:physiology; D046508:Culture Techniques; D000242:Cyclic AMP / Q000378:metabolism; D004285:Dogs; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism / Q000502:physiology; D005260:Female; D008297:Male; D008564:Membrane Potentials / Q000502:physiology; D009068:Movement / Q000502:physiology; D014132:Trachea / Q000166:cytology / Q000378:metabolism / Q000502:physiology*
chemical_list: D000242:Cyclic AMP; D015740:Calcitonin Gene-Related Peptide