Pathway and kinetics of vitellogenin-gold internalization in the Xenopus oocyte.
After in vitro incubation of Xenopus oocytes with vitellogenin (VTG)-gold conjugate, the gold particles are distributed on the whole plasma membrane. Their concentration in coated pits still occurs at 0 degrees C. At +20 degrees C the label quickly (30 sec) appears in multi-vesicular endosomes (MVE) which segregate together with primary endocytic vesicles into distinct clusters below the plasma membrane. From this step up to crystallization of the yolk platelets, the gold particles stay in the same compartment. During 5.5 h the label progressively increases along the MVE membrane, first (1.5 h) by fusion of primary endocytic vesicles with consecutively enlarging endosomes, then (4 h) by decreasing of the MVE membrane. As concerns the yolk platelet formation, concentration of primordial yolk platelets (PYP) occurs at 5.5 h from the incubation onset, the labeling of preexisting yolk platelets starts at 7 h, while crystallization of PYP begins only after 12-13 h. Our results indicate that VTG receptors are not preclustered in coated pits and their lateral translation is not inhibited at 0 degrees C. The yolk protein processing takes place within one compartment only. The VTG condensation begins with a long concentration phase of receptor-VTG complexes still integrated in the endosome membrane. It occurs in MVE by: i) a repeated fusion of primary endocytic vesicles; ii) removing part of the endosome membrane by internal vesiculation. Fusion between endosomes occurs only after VTG has dissociated from its receptors and VTG dissociates only when when the density of the VTG-receptor complexes in the endosome membrane is sufficient. Crystallization begins after a 7-8 h delay. The endosome migration into the oocyte is also controlled by the binding of VTG to its receptors. Our results also demonstrate that binding of VTG colloidal gold modifies neither the vitellogenic pathway nor the duration of the vitellogenin internalization. However when vitellogenin is bound to colloidal gold, dissociation of ligand-receptor complexes is delayed because the amount of ligand in the incubation medium is necessarily low.
Busson
S
S
Centre de Biologie Cellulaire du CNRS, Ivry-sur-Seine, France.
Ovtracht
L
L
Gounon
P
P
D000818:Animals; D004527:Egg Proteins; D004705:Endocytosis / Q000502:physiology; D005260:Female; D006046:Gold; D006651:Histocytochemistry; D066298:In Vitro Techniques; D007700:Kinetics; D008854:Microscopy, Electron; D009865:Oocytes / Q000378:metabolism*; D009866:Oogenesis / Q000502:physiology; D011956:Receptors, Cell Surface / Q000378:metabolism; D014819:Vitellogenins / Q000378:metabolism*; D014981:Xenopus / Q000378:metabolism*
D004527:Egg Proteins; D011956:Receptors, Cell Surface; D014819:Vitellogenins; C030474:vitellogenin receptor; D006046:Gold