Rescue of infectious virus from nonproducer Rous cells by chick cellular DNA.
The rescue of infectious virus from nonproducer BH RSV(-) cells by chick cellular DNA was attempted in order to investigate the functional state of endogenous and exogenous retroviral genes integrated within the cellular DNA. No infectious virus was rescued by transfection with DNAs of chick helper factor (chf)-negative chick embryo cells (CEC), chf-positive CEC or uninfected CEC producing endogenous Rous associated virus (RAV-0). On the other hand, infectious Rous viruses with the phenotype of RAV-0 and RAV-1 were rescued by transfection with DNAs of CEC which had been infected with RAV-0 and RAV-1. From these results, it seems that exogenous retroviral genes integrated in the cellular DNA are expressed rather easily by transfection while those present endogenously are not.
Ogura
H
H
Department of Virology, Okayama University Medical School, Japan.
Fujiwara
T
T
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics / Q000302:isolation & purification*; D002461:Cell Line, Transformed; D002642:Chick Embryo; D003370:Coturnix; D004279:DNA, Viral; D014162:Transfection / Q000502:physiology*
D004279:DNA, Viral