Expression of A-type PDGF receptor in cytoplasm of tumor cell lines synthesizing PDGF.
Two tumor cell lines, WM 266-4 melanoma and SW 707 colorectal carcinoma, both of which synthesize platelet-derived growth factor (PDGF) but do not express cell surface PDGF receptor, were tested for the presence of intracytoplasmic PDGF receptor. Using a novel method of intracytoplasmic (newly synthesized), receptor precipitation by the growth factor, we identified a 125,000 molecular weight protein crosslinked with 125I-labeled PDGF in a 140,000 molecular weight complex. In vitro translation of the corresponding mRNA revealed a 125,000 molecular weight product which presumably represents the A-type PDGF receptor. Exogenous PDGF was found to activate synthesis of RNA and cell proliferation.
Rakowicz-Szulczynska
E M
EM
Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104.
Koprowski
H
H
D002455:Cell Division / Q000187:drug effects; D002843:Chromatin / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000032:analysis / Q000378:metabolism*; D003593:Cytoplasm / Q000032:analysis / Q000378:metabolism; D006801:Humans; D008545:Melanoma / Q000032:analysis / Q000378:metabolism*; D008970:Molecular Weight; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis* / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000096:biosynthesis / Q000235:genetics; D012335:RNA, Ribosomal / Q000096:biosynthesis; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000235:genetics; D017479:Receptors, Platelet-Derived Growth Factor; D014407:Tumor Cells, Cultured
D002843:Chromatin; D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D012335:RNA, Ribosomal; D011956:Receptors, Cell Surface; D017479:Receptors, Platelet-Derived Growth Factor