title: Human papillomavirus type 56: a new virus detected in cervical cancers.
abstract: A new human papillomavirus type (HPV-56) was identified by low stringency Southern blot analysis with an HPV-31 DNA probe, in a cervical intraepithelial neoplasm (CIN). The DNA of this virus was molecularly cloned and shown to be a new HPV type based on the absence of cross-reactivity to HPV types 1 to 55 under high-stringency hybridization conditions. At low stringency, HPV-56 was most related to HPV types 30 and 45. The deduced organization of the open reading frames of HPV-56, from hybridization and partial nucleotide sequence analyses, reveals a typical HPV genome. HPV-56 was detected in two of 464 normal cervical tissues, in five of 227 cervical condylomas and CIN, and in two of 84 invasive cancers of the cervix.
authors.affiliation: Molecular Diagnostics Research, Life Technologies, Inc., Gaithersburg, Maryland 20877.
authors.forename: A T
authors.initials: AT
authors.lastname: Lörincz
authors.forename: A P
authors.initials: AP
authors.lastname: Quinn
authors.forename: M D
authors.initials: MD
authors.lastname: Goldsborough
authors.forename: P
authors.initials: P
authors.lastname: McAllister
authors.forename: G F
authors.initials: GF
authors.lastname: Temple
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins; D005260:Female; D005814:Genes, Viral; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D002583:Uterine Cervical Neoplasms / Q000382:microbiology*; D014764:Viral Proteins / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; C052603:E6 protein, Human papillomavirus type 18; D009856:Oncogene Proteins, Viral; D014764:Viral Proteins; D015678:Viral Structural Proteins