title: cis-acting DNA sequence requirements for P-element transposition.
abstract: The P transposable element of Drosophila melanogaster has a complex array of cis-acting DNA sequences necessary for efficient transposition. At the 3' end these sequences extend over more than 150 bp and include 11- and 31-bp sequences found repeated in inverted orientation at the 5' end. The P element's 5' end, however, cannot function as its 3' end. When two 3' P-element ends are present, the more proximal end is used preferentially. We found also that the duplication of the target site does not appear to play a role in forward transposition.
authors.affiliation: Department of Biochemistry, University of California Berkeley 94720.
authors.forename: M C
authors.initials: MC
authors.lastname: Mullins
authors.forename: D C
authors.initials: DC
authors.lastname: Rio
authors.forename: G M
authors.initials: GM
authors.lastname: Rubin
mesh_terms: D000818:Animals; D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000378:metabolism; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics; D005854:Germ Cells; D008969:Molecular Sequence Data; D009154:Mutation; D012333:RNA, Messenger / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D014170:Transformation, Genetic
chemical_list: D004251:DNA Transposable Elements; D012333:RNA, Messenger; D015500:Chloramphenicol O-Acetyltransferase