Recombinant human erythropoietin produced by Namalwa cells.
To establish a practical exogenous gene expression system in human cells, a cDNA coding for human erythropoietin (EPO) was expressed in human B-lymphoblastoid Namalwa cells. The Namalwa-derived recombinant EPO was purified from the culture fluid by a simple three-step procedure. The Namalwa EPO showed an equivalent activity in vivo to that of human urinary EPO. Oligosaccharide structure analyses suggested that almost all N-linked oligosaccharide chains of Namalwa EPO are shared by urinary EPO. The two major N-linked oligosaccharides of Namalwa EPO were fucose-containing tetraantennary and fucose-containing triantennary structures.
Yanagi
H
H
Takarazuka Research Center, Sumitomo Chemical Co., Ltd., Hyogo, Japan.
Yoshima
T
T
Ogawa
I
I
Okamoto
M
M
D000818:Animals; D002240:Carbohydrate Sequence; D002460:Cell Line; D002845:Chromatography; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000235:genetics; D017886:Durapatite; D004921:Erythropoietin / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D006882:Hydroxyapatites; D007501:Iron / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009844:Oligosaccharides / Q000302:isolation & purification; D010957:Plasmids; D051381:Rats; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D015183:Restriction Mapping
D006882:Hydroxyapatites; D009844:Oligosaccharides; D011994:Recombinant Proteins; D004921:Erythropoietin; D004247:DNA; D017886:Durapatite; D007501:Iron