Amino acid substitutions in albumin variants found in Brazil.
Conventional horizontal starch-gel electrophoresis in four buffer systems and structural studies were performed on four albumin variants, and the findings were compared with similar previous data. Albumins Coari I and Porto Alegre I have a previously unreported amino acid substitution (glutamic acid replaced by lysine at position 358, denoted 358 Glu----Lys). The alteration in albumin Porto Alegre II (501 Glu----Lys) is the same as that found for three alloalbumins of Asiatic origin, designated Vancouver, Birmingham, and Adana. Albumin Oriximiná I has the same exchange as albumin Maku (541 Lys----Glu). Some of these findings can be explained only by the occurrence of independent mutations at the same site in the albumin gene. They also point to a third cluster of mutations in that gene, indicating hypermutability in some of its segments.
Arai
K
K
Department of Biology, Indiana University, Bloomington 47405.
Huss
K
K
Madison
J
J
Putnam
F W
FW
Salzano
F M
FM
Franco
M H
MH
Santos
S E
SE
Freitas
M J
MJ
D000595:Amino Acid Sequence; D001938:Brazil; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003488:Cyanogen Bromide; D004592:Electrophoresis, Starch Gel; D014644:Genetic Variation; D005971:Glutamates; D018698:Glutamic Acid; D006455:Hemoglobins, Abnormal; D006801:Humans; D008239:Lysine; D008969:Molecular Sequence Data; D010446:Peptide Fragments; D011110:Polymorphism, Genetic; D012709:Serum Albumin / Q000235:genetics*; D014357:Trypsin
D005971:Glutamates; D006455:Hemoglobins, Abnormal; D010446:Peptide Fragments; D012709:Serum Albumin; C058914:hemoglobin Coari I; C058917:hemoglobin Oriximina I; C058915:hemoglobin Porto Alegre I; C058916:hemoglobin Porto Alegre II; D018698:Glutamic Acid; D014357:Trypsin; D008239:Lysine; D003488:Cyanogen Bromide