title: [Inorganic pyrophosphatase of rat liver cytosol. Isolation and properties].
abstract: An electrophoretically homogeneous preparation of rat liver cytosolic pyrophosphatase was obtained by a combination of chromatographic methods. The enzyme molecule is made up of two identical subunits, each about 38 kD. The enzyme is activated by Mg2+ and strongly inhibited by Ca2+. Both cations are bound on a time scale of minutes. Ca2+ binding occurs in two steps. EGTA-like metal chelators activate pyrophosphatase, presumably due to the removal of trace amounts of Ca2+ from solutions.
authors.forename: I N
authors.initials: IN
authors.lastname: Smirnova
authors.forename: A A
authors.initials: AA
authors.lastname: Baĭkov
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D002614:Chelating Agents / Q000494:pharmacology; D003600:Cytosol / Q000201:enzymology*; D004533:Egtazic Acid; D004789:Enzyme Activation; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008274:Magnesium / Q000494:pharmacology; D008970:Molecular Weight; D011755:Pyrophosphatases / Q000032:analysis / Q000302:isolation & purification*; D051381:Rats
chemical_list: D002614:Chelating Agents; D004533:Egtazic Acid; D011755:Pyrophosphatases; D008274:Magnesium; D002118:Calcium
vernacular_title: Neorganicheskaia pirofosfataza tsitozolia pecheni krysy. vydelenie i svoĭstva.