The immunopotentiating property of lipophilic muramyl dipeptide and its molecular state in liposomal membranes: plaque-forming cell responses and ESR studies.
The immunopotentiating property of spin-labeled lipophilic muramyl dipeptide (SL-MDP) in liposomes was studied as to the plaque-forming cell (PFC) response to glycophorin, as an antigen, in membranes. The effect of SL-MDP depended on the densities of both SL-MDP itself and the antigen. The addition of the optimal amount of SL-MDP to liposomes containing a low density (0.016 mol%) of the antigen increased the PFC response by three times, whereas the presence of SL-MDP in optimal antigen density (0.032 0.127 mol%) membranes was rather inhibitory. In these liposomes, the amounts and molecular states of SL-MDP were determined from ESR spectra and are discussed in connection with its immunopotentiating property.
Manabe
H
H
Department of Health Chemistry, School of Pharmaceutical Science, Showa University, Tokyo.
Utsumi
H
H
Kusama
T
T
Hamada
A
A
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000494:pharmacology*; D000276:Adjuvants, Immunologic; D000941:Antigens / Q000276:immunology; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004578:Electron Spin Resonance Spectroscopy; D006021:Glycophorins / Q000032:analysis; D006462:Hemolytic Plaque Technique; D008081:Liposomes / Q000032:analysis*; D008567:Membranes, Artificial
D000276:Adjuvants, Immunologic; D000941:Antigens; D006021:Glycophorins; D008081:Liposomes; D008567:Membranes, Artificial; D000119:Acetylmuramyl-Alanyl-Isoglutamine