Expression of virally transduced mouse tyrosinase in tyrosinase-negative chick embryo melanocytes in culture.
A cDNA encoding mouse tyrosinase was inserted into a plasmid containing the provirus of a replication competent avian leukosis virus (ALV). A viral stock produced from the plasmid was used to infect cultured tyrosinase-negative (ca/ca) unpigmented chick embryo melanocytes. Five days after infection many cells were producing very dark discrete melanosomes.
Whitaker
B A
BA
School of Biological Sciences, University of Nebraska, Lincoln 68588-0118.
Frew
T J
TJ
Greenhouse
J J
JJ
Hughes
S H
SH
Yamamoto
H
H
Takeuchi
T
T
Brumbaugh
J A
JA
D000818:Animals; D001354:Avian Leukosis Virus / Q000235:genetics; D004156:Catechol Oxidase / Q000378:metabolism*; D002478:Cells, Cultured; D002642:Chick Embryo; D015870:Gene Expression; D008544:Melanocytes / Q000201:enzymology*; D051379:Mice; D014442:Monophenol Monooxygenase / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids; D014161:Transduction, Genetic
D004156:Catechol Oxidase; D014442:Monophenol Monooxygenase