The muridae glyceraldehyde-3-phosphate dehydrogenase family.
Although only one gene is known to be functional, numerous glyceraldehyde-3-phosphate dehydrogenase (GAPDH) related sequences are scattered throughout Mus musculus and Rattus rattus genomes. In this report we show that: (1) GAPDH pseudogenes are repeated to comparable extents, at least 400 copies, in 12 other Muridae species; (2) the complete, or nearly so, sequence of GAPDH messenger RNA is amplified, and a high proportion, if not all of these copies, are intronless; (3) GAPDH pseudogenes are preferentially located in heavily methylated and DNAse I-insensitive regions of chromatin; and (4) the presence of atypical GAPDH-related mRNAs in different cellular contexts raises the possibility that more than one GAPDH gene is transcribed.
Riad-el Sabrouty
S
S
UA CNRS, Laboratoire de Biologie Moléculaire, Montpellier, France.
Blanchard
J M
JM
Marty
L
L
Jeanteur
P
P
Piechaczyk
M
M
D000818:Animals; D005075:Biological Evolution; D002460:Cell Line; D004247:DNA / Q000235:genetics; D004251:DNA Transposable Elements; D003850:Deoxyribonuclease I; D005784:Gene Amplification; D005796:Genes; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics*; D008745:Methylation; D009115:Muridae / Q000235:genetics*; D011544:Pseudogenes; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D013045:Species Specificity; D014158:Transcription, Genetic
D004251:DNA Transposable Elements; D012333:RNA, Messenger; D004247:DNA; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D003850:Deoxyribonuclease I