title: [Immunoenzyme analysis of adenovirus hexons. Indication and characteristics of rabbit and mouse antibodies against hexons].
abstract: An enzyme-linked immunosorbent assay (ELISA) was developed for analysis of rabbit and mouse IgG antibodies specific to adenoviral hexon. The anti-hexon antibodies were detected by capture with purified hexon coated onto polystyrene microtiter plates and visualizing them by respective anti-IgG horseradish peroxidase conjugates. In the sera from hyperimmunized rabbits and mice as well as in the mouse ascite fluids the ELISA procedure revealed primarily type-specific (epsilon) and genus-specific (alpha) antigenic determinants in hexon but not those of intermediate specificities.
authors.forename: S N
authors.initials: SN
authors.lastname: Khil'ko
authors.forename: M A
authors.initials: MA
authors.lastname: Kirasova
authors.forename: V R
authors.initials: VR
authors.lastname: Isakova
authors.forename: M P
authors.initials: MP
authors.lastname: Burgasova
authors.forename: E K
authors.initials: EK
authors.lastname: Kiseleva
mesh_terms: D000256:Adenoviridae / Q000276:immunology*; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D000259:Aviadenovirus / Q000276:immunology*; D002213:Capsid / Q000276:immunology; D036022:Capsid Proteins; D002642:Chick Embryo; D002645:Chickens; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D007124:Immunoenzyme Techniques; D051379:Mice; D011817:Rabbits
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; C018315:hexon capsid protein, Adenovirus
vernacular_title: Immunofermentnyĭ analiz geksona adenovirusov. Indikatsiia i kharakteristika krolich'ikh i myshinykh antitel protiv geksonov.