Effect of converting enzyme inhibition on renin synthesis and secretion in mice.
We have investigated the relative importance of renal renin stores and de novo synthesis during stimulation of renin secretion and the role of transcription and posttranscriptional factors in providing increased synthesis of renin. When enalapril was administered to previously untreated mice, plasma renin concentration increased 40-fold within 1.5 hours, and remained at a high level for the 8 days of the experiment. Renal renin decreased by 82% after 24 hours and thereafter increased to levels higher than controls. Calculations of renin turnover, based on data for the rate of metabolism of renin in plasma, indicated that most of the renin released in the first 24 hours could be accounted for by the decrease in renal renin stores, indicating that de novo synthesis played only a minor role. After 24 hours, however, when both plasma renin concentration and renal renin increased, the calculated rate of renin synthesis increased to nearly 40 times the rate in controls. When enalapril was administered to mice that had been depleted of plasma and renal renin by chronic sodium loading, plasma renin concentration increased markedly within 1.5 hours, but to only half the level achieved in the previously untreated mice. No decrease in renal renin occurred, suggesting that the renal renin remaining after chronic sodium loading was not available for release. Renal renin messenger RNA increased 4.5-fold after 6 hours, and after 8 days had increased to 5.0 times the level at day 0. The increase in calculated rate of renin synthesis was maximal between 5 and 8 days, when it was 54 times greater than at day 0. During enalapril treatment, there were marked increases in the granulation of the juxtaglomerular cells and in the amount of rough endoplasmic reticulum and Golgi apparatus they contained. These results suggest that posttranscriptional factors play a major role in determining the rate of renin synthesis.
Barrett
G L
GL
Department of Physiology, University of Melbourne, Parkville, Australia.
Morgan
T O
TO
Smith
M
M
Alcorn
D
D
Aldred
P
P
D000704:Analysis of Variance; D000818:Animals; D004656:Enalapril / Q000494:pharmacology*; D005438:Fludrocortisone / Q000494:pharmacology; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D007668:Kidney / Q000502:physiology* / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D007703:Peptidyl-Dipeptidase A / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics; D012044:Regression Analysis; D012045:Regulatory Sequences, Nucleic Acid; D012083:Renin / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D012636:Secretory Rate / Q000187:drug effects; D012965:Sodium Chloride / Q000494:pharmacology; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects
D012333:RNA, Messenger; D012965:Sodium Chloride; D004656:Enalapril; D007703:Peptidyl-Dipeptidase A; D012083:Renin; D005438:Fludrocortisone