Radioimmunoassay of delta-toxin from B. thuringiensis: correlation with bioassay.
A radioimmunoassay procedure, developed for delta-toxin of B. thuringiensis, is applicable to the assay of the toxin's bioactivity. When delta-toxin is inactivated to different degrees by heat treatment, the amount of toxin as determined by radioimmunoassay is proportionally decreased. It thus appears that the delta-toxin antibody may recognize and bind to the conformation in the molecule associated with its insecticidal activity.
Tyski
S
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Department of Bacteriology, National Institute of Hygiene, Warsaw, Poland.
D000818:Animals; D001413:Bacillus thuringiensis; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D001681:Biological Assay; D004731:Endotoxins / Q000032:analysis* / Q000494:pharmacology; D006460:Hemolysin Proteins; D006358:Hot Temperature; D007306:Insecticides; D009036:Moths; D011863:Radioimmunoassay
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; C030956:insecticidal crystal protein, Bacillus Thuringiensis