Effect of Biostim (RU 41.740) on natural killer cell generation from bone marrow precursors.
We have evaluated the possible effect of RU 41.740 (Biostim), a mixture of two glycoproteins extracted from K. pneumoniae, on the in vitro interleukin-2 (IL-2)-induced generation of NK cells from bone marrow (BM) precursors and on the in vivo reconstitution of splenic NK activity in lethally irradiated (9 Gy) and BM reconstituted mice. Our results show that RU 41.740 is able to augment the generation of NK cells when added (1-0.01 micrograms/ml) to normal or 5-fluorouracil-resistant BM, cultured in the presence of recombinant IL-2. Also, in vivo treatment of lethally irradiated mice, transplanted with syngeneic BM cells, with RU 41.740 (1-0.1 mg/kg i.v.) from day 0 through day 4 after BM graft, resulted in a significant augmentation of NK activity reconstitution.
Migliorati
G
G
Institute of Pharmacology, School of Medicine, University of Perugia, Italy.
Guidi
G
G
Cannarile
L
L
Riccardi
C
C
D000818:Animals; D001426:Bacterial Proteins / Q000494:pharmacology*; D001853:Bone Marrow / Q000187:drug effects*; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation; D007376:Interleukin-2 / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000187:drug effects*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D014916:Whole-Body Irradiation
D001426:Bacterial Proteins; D007376:Interleukin-2; C037567:glycoprotein, Klebsiella pneumoniae