Alpha-1-adrenergic stimulation of phosphoinositide breakdown in cultured neonatal rat ventricular myocytes.
The regulation of and the intracellular events following alpha 1-adrenergic receptor stimulation in the myocardium still remain to be disclosed. The effect of alpha 1-adrenergic stimulation on phosphoinositide breakdown was studied in cultured neonatal rat ventricular myocytes. Phenylephrine (30 microM) stimulated inositolphosphates formation, but only in the presence of 10 mM LiCl this could be measured. The increase was antagonized by prazosin (1 microM) but not by propranolol (1 microM). The variability in proportional distribution of the three inositolphosphates is discussed.
Meij
J T
JT
Department of Biochemistry I, Erasmus University Rotterdam, The Netherlands.
Lamers
J M
JM
D000818:Animals; D002478:Cells, Cultured; D002712:Chlorides / Q000494:pharmacology; D015544:Inositol 1,4,5-Trisphosphate; D007295:Inositol Phosphates / Q000378:metabolism; D008094:Lithium / Q000494:pharmacology; D018021:Lithium Chloride; D009206:Myocardium / Q000378:metabolism*; D010656:Phenylephrine / Q000494:pharmacology; D010716:Phosphatidylinositols / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011942:Receptors, Adrenergic, alpha / Q000187:drug effects / Q000502:physiology*; D010738:Type C Phospholipases / Q000378:metabolism
D002712:Chlorides; D007295:Inositol Phosphates; D010716:Phosphatidylinositols; D011942:Receptors, Adrenergic, alpha; C002647:inositol 1-phosphate; D010656:Phenylephrine; C044259:inositol 1,4-bis(phosphate); D015544:Inositol 1,4,5-Trisphosphate; D008094:Lithium; D010738:Type C Phospholipases; D018021:Lithium Chloride