Long-term growth of human WGA-activated T-lymphocytes without feeder cells.
Human wheat germ agglutinin (WGA)-activated T-lymphocytes from either peripheral blood or spleen could be propagated for several weeks in the presence of culture supernatants from human mononuclear cells obtained after a 1-day stimulation with WGA and after a subsequent 3-day culture without mitogen. The continuous T-cell growth in this culture system required alternate exposition of the cells to the above supernatants, both with and without WGA, but proceeded in the absence of feeder cells. After 3 weeks of propagation most cells displayed the CD4+ phenotype, expressed IL2 receptors, and responded to PHA, Con A, and WGA. It has been shown that WGA-activated T-cells could be cloned by limiting dilution and propagated using the above culture system.
Siwicki
J K
JK
Department of Immunology, Marie Skłodowska-Curie Memorial Cancer Center, Warsaw, Poland.
D015496:CD4-Positive T-Lymphocytes / Q000166:cytology; D002478:Cells, Cultured; D003470:Culture Media; D006801:Humans; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000502:physiology; D008213:Lymphocyte Activation; D010835:Phytohemagglutinins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D014909:Wheat Germ Agglutinins / Q000494:pharmacology
D003470:Culture Media; D007376:Interleukin-2; D010835:Phytohemagglutinins; D014909:Wheat Germ Agglutinins