Microcalorimetric studies of conformational transitions of ferricytochrome c in acidic solution.
The conformational transitions of ferricytochrome c in acidic solutions with different NaCl concentrations have been studied by scanning and isothermal microcalorimetry. It is shown that ferricytochrome c adopts three different forms which are realized under the considered conditions: native, denatured (unfolded) and a compact native-like form with unique tertiary structure. The thermodynamic parameters of the corresponding transitions have been measured and the changes in the number of bound ligands (H+ and Cl-), accompanying these transitions, have been determined by analyzing the temperature, pH and ionic strength dependence of these parameters.
Potekhin
S
S
Institute of Protein Research, U.S.S.R. Academy of Sciences, Moscow Region.
Pfeil
W
W
D002151:Calorimetry; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003574:Cytochrome c Group; D006863:Hydrogen-Ion Concentration; D011487:Protein Conformation; D012965:Sodium Chloride; D012996:Solutions; D013696:Temperature; D013816:Thermodynamics
D003574:Cytochrome c Group; D012996:Solutions; D012965:Sodium Chloride