Effects of bradykinin on PC-12 cell differentiation.
PC-12 cells are used as a model for neuronal differentiation because they assume a neuronal phenotype, including the extension of neurites, when exposed to nerve growth factor (NGF). The present results show that bradykinin (BK) also causes PC-12 cells to extend neurites. In addition, BK potentiates the neurite-extending effect of nerve growth factor (NGF), an action which is attenuated by a BK antagonist. The potentiation of neurite extension produced by the combination of BK and NGF may be mediated at the receptor level, as indicated by an NGF-induced alteration of BK binding.
Kozlowski
M R
MR
Department of Screening and Biochemical Research, Bristol-Myers Company, Wallingford, CT 06492-7660.
Rosser
M P
MP
Hall
E
E
Longden
A
A
D000818:Animals; D001369:Axons / Q000187:drug effects; D001920:Bradykinin / Q000378:metabolism / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D009414:Nerve Growth Factors / Q000378:metabolism / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects*; D051381:Rats; D018002:Receptors, Bradykinin; D011956:Receptors, Cell Surface / Q000378:metabolism; D017475:Receptors, Nerve Growth Factor; D017981:Receptors, Neurotransmitter / Q000378:metabolism; D014407:Tumor Cells, Cultured
D009414:Nerve Growth Factors; D018002:Receptors, Bradykinin; D011956:Receptors, Cell Surface; D017475:Receptors, Nerve Growth Factor; D017981:Receptors, Neurotransmitter; D001920:Bradykinin