High-performance liquid chromatographic determination of bromazepam in human plasma.
An assay using high-performance liquid chromatography has been developed for the determination of bromazepam in plasma. After a single-step extraction from basified samples with dichloromethane, using decarboxyloflazepate as an internal standard, samples were analysed using a reversed-phase Nova Pak 5-microns column with a mobile phase of methanol - phosphate buffer (60 + 40) adjusted to pH 7.6. The drugs were detected at 239 nm and the limit of detection was found to be 3 micrograms l-1 for bromazepam. The method is simple, rapid and sensitive and permits bromazepam levels in clinical and pharmacokinetic studies to be monitored.
Boukhabza
A
A
Lugnier
A A
AA
Kintz
P
P
Tracqui
A
A
Mangin
P
P
Chaumont
A J
AJ
D014151:Anti-Anxiety Agents / Q000097:blood*; D001960:Bromazepam / Q000097:blood*; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D013056:Spectrophotometry, Ultraviolet
D014151:Anti-Anxiety Agents; D001960:Bromazepam