Spontaneous excretion of D-alanine in urine in mutant mice lacking D-amino-acid oxidase.
Urine from mutant mice lacking D-amino-acid oxidase contained a large amount of alanine compared with that from normal mice. Urinary alanine of the mutant mice was sensitive to D-amino-acid oxidase. H.p.l.c. showed that about 94% of the urinary alanine had the D-configuration. These results suggest that D-amino-acid oxidase functions to decompose D-amino acid(s) in normal mice.
Konno
R
R
Department of Microbiology, Dokkyo University School of Medicine, Tochigi, Japan.
Nagata
Y
Y
Niwa
A
A
Yasumura
Y
Y
D000409:Alanine / Q000652:urine*; D000818:Animals; D003605:D-Amino-Acid Oxidase / Q000172:deficiency*; D051379:Mice; D008817:Mice, Mutant Strains; D013237:Stereoisomerism
D003605:D-Amino-Acid Oxidase; D000409:Alanine