title: Direct ESR measurement of free radicals in mouse pancreatic lesions.
abstract: In this experiment, free radicals in the pancreas of endotoxemia and ethionine induced acute pancreatitis in mice were attempted to be detected directly by ESR spectroscopy, using 77 K freeze-trapping and 25 degrees C DMPO spin trapping techniques. In the 77 K freeze-trapping method, Mn (II) ion and R-00. radical were detected in endotoxemia and ethionine induced pancreatic lesions. The heme-NO radical was observed at 6 and 24 h after isolation of the normal pancreas, and signal intensity was increased with time. This finding supports that ESR spectroscopy is a useful method for detecting the tissue degeneration process from ischemia to necrosis. Using the DMPO spin trapping technique (25 degrees C), 6-line was detected at 6 h after intraperitoneal administration of E. coli in the model of endotoxemia, and 3- and 6-lines and a signal suggestive of DMPO-OH adduct were noted at 12 and 24 h in ethionine pancreatitis. These findings suggest that impaired pancreatic tissues exist in a considerably oxidative environment and oxygen derived free radicals may be considered to play an important role in the development of pancreatic lesions.
authors.affiliation: First Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Nonaka
authors.forename: T
authors.initials: T
authors.lastname: Manabe
authors.forename: N
authors.initials: N
authors.lastname: Asano
authors.forename: T
authors.initials: T
authors.lastname: Kyogoku
authors.forename: K
authors.initials: K
authors.lastname: Imanishi
authors.forename: K
authors.initials: K
authors.lastname: Tamura
authors.forename: T
authors.initials: T
authors.lastname: Tobe
authors.forename: Y
authors.initials: Y
authors.lastname: Sugiura
authors.forename: K
authors.initials: K
authors.lastname: Makino
mesh_terms: D000818:Animals; D004578:Electron Spin Resonance Spectroscopy; D004731:Endotoxins; D004926:Escherichia coli; D005001:Ethionine; D005260:Female; D005609:Free Radicals; D051379:Mice; D008807:Mice, Inbred BALB C; D010179:Pancreas / Q000473:pathology*; D010195:Pancreatitis / Q000139:chemically induced / Q000473:pathology*; D012016:Reference Values; D014115:Toxemia / Q000473:pathology*
chemical_list: D004731:Endotoxins; D005609:Free Radicals; D005001:Ethionine