Antibodies to the GTP binding protein, Go, antagonize noradrenaline-induced calcium current inhibition in NG108-15 hybrid cells.
The voltage-dependent calcium current in chemically differentiated NG108-15 cells is depressed by noradrenaline acting on alpha-adrenoreceptors. The response is absent in cells pretreated with pertussis toxin, implicating the involvement of a G-protein. To identify this G-protein, we have studied the response to noradrenaline in cells preinjected with antibodies specific for two G-proteins, Gi and Go. Cells injected with the Gi antibody responded normally to noradrenaline. In contrast, the response to noradrenaline in cells injected with the Go antibody was markedly attenuated. We conclude that Go is employed in coupling alpha-adrenoreceptors to the calcium channels in NG108-15 cells.
McFadzean
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I
Department of Pharmacology, University College London, England.
Mullaney
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I
Brown
D A
DA
Milligan
G
G
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D002118:Calcium / Q000378:metabolism* / Q000502:physiology; D015220:Calcium Channels / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure; D002463:Cell Membrane Permeability / Q000187:drug effects / Q000502:physiology; D004553:Electric Conductivity / Q000187:drug effects / Q000502:physiology; D019204:GTP-Binding Proteins / Q000276:immunology* / Q000378:metabolism; D005910:Glioma / Q000473:pathology; D006822:Hybrid Cells / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D051379:Mice; D009447:Neuroblastoma / Q000473:pathology; D009638:Norepinephrine / Q000494:pharmacology*; D037342:Pertussis Toxin; D051381:Rats; D011942:Receptors, Adrenergic, alpha / Q000378:metabolism / Q000502:physiology; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D000906:Antibodies; D015220:Calcium Channels; D011942:Receptors, Adrenergic, alpha; D010566:Virulence Factors, Bordetella; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins; D002118:Calcium; D009638:Norepinephrine