title: Determination of gold-labelled surface receptors on single cells by X-ray microanalysis.
abstract: A standardless X-ray microanalytical procedure has been developed to determine the number of gold-labelled surface receptors on whole single cells. The effect of the injection of K2PtCl4 into mice on gold-labelled concanavalin A (Con A) receptors on peritoneal macrophages was examined with an energy dispersive X-ray detector in an SEM. The numbers of gold particles seen in electron micrographs and estimated by fluorescence photometric measurements of fluorescein isothiocyanate-labelled Con A receptors were correlated with the X-ray microanalytical results.
authors.affiliation: GDR Academy of Sciences, Central Institute of Microbiology and Experimental Therapy.
authors.forename: T
authors.initials: T
authors.lastname: Peschke
authors.forename: K
authors.initials: K
authors.lastname: Augsten
authors.forename: F
authors.initials: F
authors.lastname: Sasama
authors.forename: A
authors.initials: A
authors.lastname: Gabert
mesh_terms: D000818:Animals; D004577:Electron Probe Microanalysis / Q000379:methods*; D006046:Gold / Q000032:analysis*; D008264:Macrophages / Q000032:analysis; D008297:Male; D051379:Mice; D008855:Microscopy, Electron, Scanning; D011956:Receptors, Cell Surface / Q000032:analysis*; D011952:Receptors, Concanavalin A / Q000032:analysis
chemical_list: D011956:Receptors, Cell Surface; D011952:Receptors, Concanavalin A; D006046:Gold