Cloning of the virulence regulatory (vir) locus of Bordetella pertussis and its expression in B. bronchiseptica.
Hybridisation with cosmid pRMB2, containing the vir locus of bordetella pertussis, showed that the Tn5 insertion in B. pertussis BP347 (Vir-) was located with 1 2.7 kB EcoRI fragment. When subcloned, this fragment alone was unable to complement BP347, but a larger 8.0 kb region which included the 2.7 kb EcoRI fragment, did restore expression of virulence associated properties to BP347, and to avirulent phase variants of both B. pertussis and B. bronchiseptica. EcoRI-digested DNA from stains of all four species of Bordetella showed homology to the cosmid genomic insert and to the 2.7 kb subclone, but B. avium showed a markedly different pattern of homology to the other species.
McGillivray
D M
DM
Department of Microbiology, University of Glasgow, U.K.
Coote
J G
JG
Parton
R
R
D015139:Blotting, Southern; D001884:Bordetella / Q000235:genetics* / Q000378:metabolism; D001886:Bordetella pertussis / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D003001:Cloning, Molecular; D003360:Cosmids; D004269:DNA, Bacterial / Q000235:genetics; D005809:Genes, Regulator; D005816:Genetic Complementation Test; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014774:Virulence / Q000235:genetics
D004269:DNA, Bacterial