Molecular cloning of serogroup- and serotype-specific genome segments from bluetongue virus serotype 11.
Genome segments 2, 6, 8, and 9 of bluetongue virus (BTV) serotype 11, coding for P2, NS1, NS2, and P6, respectively, were cloned into pUC 8. Sizes of segment-2 and segment-6 clones indicated partial copies (55% and 80% of full length, respectively), whereas segment 8 and 9 clones represented full-length copies. Northern blot hybridizations of the clones to the 5 United States BTV prototypic serotypes (2, 10, 11, 13, and 17) revealed segment-2 clone to be serotype-specific to BTV-11, whereas segment 6, 8, and 9 clones were able to detect all serotypes to varying degrees. All clones failed to detect the related orbivirus, epizootic hemorrhagic disease virus.
Dunn
S J
SJ
Department of Veterinary Microbiology, School of Veterinary Medicine, University of California, Davis 95616.
Oberst
R D
RD
Stott
J L
JL
Osburn
B I
BI
D000818:Animals; D015152:Blotting, Northern; D001819:Bluetongue / Q000382:microbiology; D001820:Bluetongue virus / Q000235:genetics*; D003001:Cloning, Molecular; D015342:DNA Probes; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006482:Hemorrhagic Fevers, Viral / Q000382:microbiology / Q000662:veterinary; D009693:Nucleic Acid Hybridization; D012330:RNA, Double-Stranded / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis* / Q000235:genetics; D012087:Reoviridae / Q000235:genetics*; D012088:Reoviridae Infections / Q000382:microbiology / Q000662:veterinary; D014709:Vero Cells
D015342:DNA Probes; D012330:RNA, Double-Stranded; D012367:RNA, Viral