Loss of calcium sensitivity of plasma gelsolin is associated with the presence of calcium ions during preparation.
Gelsolin is a calcium-dependent actin severing and capping protein. Calcium 'opens' the molecule to make actin binding sites accessible, but removal of calcium from the medium does not necessarily fully reverse this process. The calcium sensitivity of actin monomer binding and actin filament severing is here shown to vary considerably with the source of gelsolin and conditions of preparation. Plasma gelsolin undergoes irreversible loss of calcium sensitivity when prepared in the presence of calcium ions. This is not due solely to effects of bound calcium, because purified human plasma gelsolin expressed in E. coli and stored in calcium shows no comparable loss of calcium sensitivity when prepared or stored in calcium. These results suggest the presence of factors in plasma which, in the presence of calcium, promote an irreversible structural change in gelsolin resulting in permanent loss of calcium sensitivity.
Pope
B
B
MRC Laboratory of Molecular Biology, Cambridge, England.
Gooch
J
J
Hinssen
H
H
Weeds
A G
AG
D000199:Actins / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D002118:Calcium / Q000502:physiology*; D002135:Calcium-Binding Proteins / Q000302:isolation & purification / Q000502:physiology*; D004533:Egtazic Acid / Q000494:pharmacology; D018260:Gelsolin; D066298:In Vitro Techniques; D008840:Microfilament Proteins / Q000302:isolation & purification / Q000502:physiology*; D011994:Recombinant Proteins; D013552:Swine
D000199:Actins; D002135:Calcium-Binding Proteins; D018260:Gelsolin; D008840:Microfilament Proteins; D011994:Recombinant Proteins; D004533:Egtazic Acid; D002118:Calcium