Partial coexistence of growth hormone-releasing hormone and tyrosine hydroxylase in paraventricular neurons in rats.
Immunocytochemistry revealed growth hormone-releasing hormone (GRF)-containing cells (4-10/50 microns thick coronal sections) in the ventral portion of the medial parvicellular subdivision of the paraventricular nucleus (PVN) in the rat. In the same region we also detected about the same number of neurons containing the mRNA that encodes GRF using in situ hybridization histochemistry. Fluorescence double labelling immunohistochemistry showed the presence of GRF and tyrosine hydroxylase (TH) in the same PVN neurons. The overlap between the two populations of cells is only partial: less than half of TH-positive cells contain GRF and vice versa.
Horváth
S
S
Laboratory of Cell Biology, NIMH, Bethesda, MD.
Mezey
E
E
Palkovits
M
M
D000818:Animals; D015342:DNA Probes; D013006:Growth Hormone / Q000378:metabolism*; D007150:Immunohistochemistry; D007276:Injections, Intraventricular; D008297:Male; D009474:Neurons / Q000201:enzymology / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D010286:Paraventricular Hypothalamic Nucleus / Q000201:enzymology / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*
D015342:DNA Probes; D013006:Growth Hormone; D014446:Tyrosine 3-Monooxygenase