[Current approaches to the search for new restriction endonucleases].
The main methodological approaches to the search of new restriction endonucleases are reviewed. These methods include obtaining acellular extracts by ultrasonic desintegration of microbial cells, osmotic shock effects, the effects of organic solvents, mechanical disruption of bacterial cells, biphase division after Albertson and others. The resolving power of any method discussed depends mainly on the level of restriction endonuclease activity, the presence of nonspecific endonucleases in the biomass, the presence of exonucleases and the taxonomy of the used microorganisms.
Sokolov
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D004262:DNA Restriction Enzymes / Q000302:isolation & purification*
D004262:DNA Restriction Enzymes
Sovremennye podkhody k poisku novykh éndonukleaz restriktsii.