Two proteins of 220 kD and 230 kD bind to UV-damaged SV40 minichromosomes in irradiated monkey kidney cells.
We exposed SV40-infected monkey kidney cells to 0, 30 or 150 J/m2 of UV-radiation, and isolated viral minichromosomes at various times after irradiation. Analysis of minichromosome-associated proteins by SDS-PAGE revealed the presence of two proteins, of 220 kD and 230 kD associated with minichromosomes from irradiated cells, but not from unirradiated controls. The larger protein was the less abundant, and was most evident in preparations from more heavily irradiated cells. Neither protein was associated with minichromosomes isolated 30 min after irradiation, but were apparent in minichromosome preparations isolated 1-4 h after UV treatment.
Brown-Luedi
M L
ML
MRC Radiobiology Unit, Chilton, Didcot, Great Britain.
Brown
T C
TC
D000818:Animals; D002478:Cells, Cultured; D002875:Chromosomes / Q000378:metabolism*; D004260:DNA Repair; D000882:Haplorhini; D066298:In Vitro Techniques; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000378:metabolism*; D013539:Simian virus 40; D013997:Time Factors; D014466:Ultraviolet Rays
D009687:Nuclear Proteins