Alpha2-adrenergic receptors in rat and rabbit eye: a tritium-sensitive film autoradiography.
Using a tritium-sensitive film and [3H]-clonidine, alpha 2-adrenergic receptors were localized in entire eye sections of rabbits and rats. This radioactive agonist specifically labelled three structures in albino animals: ocular muscles, ciliary processes and retina. In pigmented animals, uvea with melanin was intensively but nonspecifically labelled. Microscopic analysis of autoradiograms obtained with iris-ciliary bodies, revealed that the major localization of binding sites was in the ciliary process epithelium. In displacement studies, [3H]-clonidine binding was inhibited by alpha 2-adrenergic compounds while alpha 1- or beta-selective drugs had no inhibitory effects.
Elena
P P
PP
Laboratoire de Pharmacologie, Faculté de Médecine, Nice, France.
Kosina-Boix
M
M
Denis
P
P
Moulin
G
G
Lapalus
P
P
D000316:Adrenergic alpha-Agonists / Q000378:metabolism; D000317:Adrenergic alpha-Antagonists / Q000378:metabolism; D000818:Animals; D001345:Autoradiography; D001667:Binding, Competitive; D003000:Clonidine / Q000378:metabolism; D005123:Eye / Q000032:analysis*; D011817:Rabbits; D051381:Rats; D011942:Receptors, Adrenergic, alpha / Q000032:analysis*
D000316:Adrenergic alpha-Agonists; D000317:Adrenergic alpha-Antagonists; D011942:Receptors, Adrenergic, alpha; D003000:Clonidine