VIP receptors and control of short circuit current in the human intestinal clonal cell line Cl.19A.
At the maximally effective concentration of 10 nM, VIP induced a marked (12.5-fold stimulation above basal), and sustained increase in short circuit current in the human intestinal epithelial cell line Cl.19A grown on permeable filters and placed in Ussing chambers. Half-maximal increase of Isc was observed for 0.1 nM VIP. This was well correlated with the VIP-stimulated adenylate cyclase activity (ED50:0.07 nM). Binding studies using 125I-VIP indicated that Cl.19A cells express a peptide-specific VIP receptor with a dissociation constant of 0.07 nM. Covalent labeling of receptors followed by SDS-PAGE analysis of membrane proteins resulted in the identification of a 63,000 dalton binding protein in Cl.19A cells.
Rouyer-Fessard
C
C
Unité de Recherche sur la Différenciation et la Neuroendocrinologie des Cellules Digestives, INSERM U178, Villejuif, France.
Augeron
C
C
Grasset
E
E
Maoret
J J
JJ
Laboisse
C L
CL
Laburthe
M
M
D000230:Adenocarcinoma; D000262:Adenylyl Cyclases / Q000378:metabolism; D001667:Binding, Competitive; D003110:Colonic Neoplasms; D004553:Electric Conductivity; D004848:Epithelium / Q000187:drug effects / Q000502:physiology; D006801:Humans; D007422:Intestines / Q000187:drug effects / Q000502:physiology*; D008970:Molecular Weight; D011964:Receptors, Gastrointestinal Hormone / Q000502:physiology*; D018005:Receptors, Vasoactive Intestinal Peptide; D014407:Tumor Cells, Cultured; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism / Q000494:pharmacology
D011964:Receptors, Gastrointestinal Hormone; D018005:Receptors, Vasoactive Intestinal Peptide; D014660:Vasoactive Intestinal Peptide; D000262:Adenylyl Cyclases