Ubiquitin expression in Neurospora crassa: cloning and sequencing of a polyubiquitin gene.
We have cloned and sequenced a polyubiquitin gene from Neurospora crassa that is organized in a four repeat-tandem array. The first repeat contains a small intron and the last is fused to an extra glutamine codon. In Northern blots, two RNA species of 1.3 kb and 0.7 kb hybridize to the isolated clone. The larger ubiquitin (UBI) transcript accumulates after partial inhibition of protein synthesis with cycloheximide, and the smaller one preferentially accumulates in conidia after germination. Unexpectedly, constitutive expression of UBI transcripts in exponentially grown mycelia is not altered by heat-shock or exposure to arsenite.
Taccioli
G E
GE
Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI), Buenos Aires, Argentina.
Grotewold
E
E
Aisemberg
G O
GO
Judewicz
N D
ND
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D003513:Cycloheximide / Q000494:pharmacology; D015342:DNA Probes; D004262:DNA Restriction Enzymes; D004271:DNA, Fungal / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D005800:Genes, Fungal; D007438:Introns; D008969:Molecular Sequence Data; D009491:Neurospora / Q000235:genetics*; D009492:Neurospora crassa / Q000235:genetics* / Q000502:physiology; D009693:Nucleic Acid Hybridization; D012331:RNA, Fungal / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014452:Ubiquitins / Q000235:genetics*
D003062:Codon; D015342:DNA Probes; D004271:DNA, Fungal; D012331:RNA, Fungal; D014452:Ubiquitins; D003513:Cycloheximide; D004262:DNA Restriction Enzymes