Isolated human bladder: evidence for an adenine dinucleotide acting on P2X-purinoceptors and for purinergic transmission.
In isolated strips of human urinary bladder detrusor muscle, ATP, alpha, beta-methylene ATP and P1,P6-diadenosine hexaphosphate caused concentration-dependent contractions. ATP was less potent than the two synthetic purine compounds and gave smaller maximum responses. Responses to ATP, P1,P6-diadenosine hexaphosphate and noncholinergic nerve stimulation were blocked following desensitization of P2X-purinoceptors by alpha,beta-methylene ATP. Thus, adenine dinucleotides can act on P2X-purinoceptors and there is an element of purinergic neuromuscular transmission in the human urinary bladder.
Hoyle
C H
CH
Department of Anatomy and Developmental Biology, University College, London, U.K.
Chapple
C
C
Burnstock
G
G
D000227:Adenine Nucleotides / Q000494:pharmacology*; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D000328:Adult; D000368:Aged; D015226:Dinucleoside Phosphates / Q000494:pharmacology; D004558:Electric Stimulation; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D009130:Muscle, Smooth / Q000187:drug effects*; D009469:Neuromuscular Junction / Q000187:drug effects; D011983:Receptors, Purinergic / Q000187:drug effects*; D009435:Synaptic Transmission / Q000187:drug effects; D001743:Urinary Bladder / Q000187:drug effects
D000227:Adenine Nucleotides; D015226:Dinucleoside Phosphates; D011983:Receptors, Purinergic; C012275:P(1),P(5)-di(adenosine-5'-)pentaphosphate; D000255:Adenosine Triphosphate; C002630:alpha,beta-methyleneadenosine 5'-triphosphate