title: Variability in gold bead density in cells. Quantitative immunocytochemistry.
abstract: Variability in gold bead distribution between individual cells was demonstrated in both pituitary melanotrophic cells immunocytochemically reacted for ACTH and in neurohypophysial terminals reacted for oxytocin-neurophysin. Gold beads were confined to the secretory granules compartment of both tissues. Density of gold beads in melanotrophic cells reacted for ACTH varied from 100-480 gold beads/microns 2. A much narrower range of gold beads distribution (460-900 gold beads/microns 2) was observed in axons of the neurohypophysis reacted with anti-oxytocin-neurophysin. These results indicate that the labeling density varies from cell to cell (as well as axon terminals) within morphologically homogeneous populations. Thus, it may reflect certain physiological differences between cells. A suggestion is being made that mean gold bead density coefficient of variation should be calculated by comparison between individual cells.
authors.affiliation: Department of Pathology, Sackler School o Medicine, Tel-Aviv University, Israel.
authors.forename: I
authors.initials: I
authors.lastname: Hammel
authors.forename: M
authors.initials: M
authors.lastname: Elmalek
authors.forename: M
authors.initials: M
authors.lastname: Castel
authors.forename: M
authors.initials: M
authors.lastname: Kalina
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000032:analysis; D000818:Animals; D002452:Cell Count; D002454:Cell Differentiation; D003594:Cytoplasmic Granules / Q000032:analysis; D006046:Gold / Q000032:analysis*; D007150:Immunohistochemistry / Q000379:methods*; D008854:Microscopy, Electron; D009481:Neurophysins / Q000032:analysis; D010121:Oxytocin / Q000032:analysis; D010316:Particle Size; D010902:Pituitary Gland / Q000032:analysis / Q000378:metabolism*; D010904:Pituitary Gland, Posterior / Q000032:analysis / Q000378:metabolism*; D013223:Statistics as Topic
chemical_list: D009481:Neurophysins; D010121:Oxytocin; D006046:Gold; D000324:Adrenocorticotropic Hormone