G.L.C.-M.S. of N-(1-deoxyalditol-1-yl)octadecylamine derivatives in the analysis of methanolysates of neoglycolipids obtained by reductive amination.
Hydrophobic conjugates of a series of aldoses have been prepared by reductive amination with octadecylamine and sodium cyanoborohydride, as model compounds for the analysis of reductively aminated oligosaccharides derived from capsular polysaccharides of Streptococcus pneumoniae. In the context of the methanolysis procedure for sugar analysis, g.l.c. and g.l.c.-m.s. (e.i.-mode) studies were carried out on the N-(1-deoxyalditol-1-yl)octadecylamine derivatives obtained after treatment with methanolic HCl, and subsequent N-acetylation and trimethylsilylation.
van Dam
J E
JE
Department of Bio-Organic Chemistry, Utrecht University, The Netherlands.
Maas
A A
AA
Kamerling
J P
JP
Vliegenthart
J F
JF
D000586:Amination; D008401:Gas Chromatography-Mass Spectrometry; D006017:Glycolipids / Q000032:analysis*; D000432:Methanol; D009844:Oligosaccharides / Q000032:analysis*; D010084:Oxidation-Reduction; D010504:Periodic Acid; D013296:Streptococcus pneumoniae / Q000032:analysis; D013402:Sugar Alcohols / Q000032:analysis*
D006017:Glycolipids; D009844:Oligosaccharides; D013402:Sugar Alcohols; D010504:Periodic Acid; C009288:metaperiodate; D000432:Methanol