Effect of novobiocin on gamma-radiation-induced DNA repair in human lymphocyte subpopulations.
1. DNA repair was measured in 3 Gy gamma-irradiated human peripheral lymphocyte subpopulations by means of nucleoid sedimentation. 2. The influence of the antibiotic, novobiocin (an inhibitor of inter alia topoisomerase II) on the repair process was investigated. 3. Repair of 33 37% of the DNA lesions induced by gamma-irradiation in enriched B lymphocyte fractions, was retarded by novobiocin. 4. Repair in enriched T lymphocyte fractions was unaffected by novobiocin.
van Rensburg
E J
EJ
Institute for Life Sciences, University of Pretoria, South Africa.
Louw
W K
WK
Engelbrecht
R I
RI
Izatt
H
H
D001402:B-Lymphocytes / Q000187:drug effects* / Q000528:radiation effects; D004260:DNA Repair / Q000187:drug effects* / Q000528:radiation effects; D005720:Gamma Rays; D006801:Humans; D066298:In Vitro Techniques; D009675:Novobiocin / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000528:radiation effects; D059005:Topoisomerase II Inhibitors
D059005:Topoisomerase II Inhibitors; D009675:Novobiocin