Short-term delay in blood processing does not alter basal or isoproterenol-stimulated cyclic AMP accumulation in human lymphocytes.
Isoproterenol-stimulated cyclic AMP (cAMP) accumulation in lymphocytes is an index of beta-adrenergic receptor functioning. It is not known if a delay between blood collection and subsequent cell preparation influences cAMP accumulation. We compared cAMP levels in lymphocytes processed from whole blood immediately after collection and following a 3.5 hour delay. There were no significant changes in either basal or stimulated levels of cAMP. This information may alleviate time constraints in certain experimental designs where immediate isolation of lymphocytes from whole blood may not be practical.
Mills
P J
PJ
Department of Psychiatry, University of California, San Diego, La Jolla 92093.
Dimsdale
J E
JE
Ziegler
M G
MG
D000328:Adult; D003080:Cold Temperature; D000242:Cyclic AMP / Q000097:blood*; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology*; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D013997:Time Factors
D000242:Cyclic AMP; D007545:Isoproterenol