title: Human erythrocytes have binding sites for beta-endorphin.
abstract: Monoiodinated human beta-endorphin was found to bind specifically to human erythrocytes. Unlabeled beta-endorphin and beta-endorphin inhibited binding, but (-)naloxone, [D-Ala2, D-Leu5]-enkephalin, and leu- and met-enkephalin did not. Immunoelectron microscopy, using rabbit anti-beta-endorphin antibody, an antirabbit IgG secondary antibody, and complexed horseradish peroxidase, revealed that at low concentrations beta-endorphin binds to the cell surface. Electron spin resonance spectroscopy showed no effect of beta-endorphin on membrane fluidity. This receptor does not appear to conform to the characteristics of an opiate receptor.
authors.forename: C O
authors.initials: CO
authors.lastname: Simpkins
authors.forename: B P
authors.initials: BP
authors.lastname: Chenet
authors.forename: Y H
authors.initials: YH
authors.lastname: Kang
authors.forename: D L
authors.initials: DL
authors.lastname: Mazorow
authors.forename: D B
authors.initials: DB
authors.lastname: Millar
authors.forename: V W
authors.initials: VW
authors.lastname: Hollis
mesh_terms: D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D008560:Membrane Fluidity; D011957:Receptors, Opioid / Q000378:metabolism*; D001615:beta-Endorphin / Q000378:metabolism*
chemical_list: D011957:Receptors, Opioid; D001615:beta-Endorphin