Insulin and IGF-I receptors in neuroblastoma cells: increases in mRNA and binding produced by glyburide.
Insulin and IGF-I binding to neuroblastoma cells (SK-N-MC) was increased by 13% and 7% respectively following a 24hr, incubation with the sulphonylurea glyburide. This increase in binding was associated with increased steady-state levels of insulin receptor and IGF-I receptor mRNA levels. Though insulin and IGF-I both stimulate glucose uptake into these cells, the increased binding following glyburide treatment was not associated with any change in glucose uptake.
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Section on Molecular and Cellular Physiology, National Institutes of Diabetes, Digestive and Kidney Diseases, Bethesda, Maryland 20892.
Shen-Orr
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LeRoith
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D003847:Deoxyglucose / Q000378:metabolism; D005905:Glyburide / Q000494:pharmacology; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D009447:Neuroblastoma / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012334:RNA, Neoplasm / Q000378:metabolism; D011972:Receptor, Insulin / Q000187:drug effects / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000378:metabolism*; D017451:Receptors, Somatomedin; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism*
D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011956:Receptors, Cell Surface; D017451:Receptors, Somatomedin; D007334:Insulin-Like Growth Factor I; D003847:Deoxyglucose; D011972:Receptor, Insulin; D005905:Glyburide