Pellicle receptors for Actinomyces viscosus type 1 fimbriae in vitro.
Actinomyces viscosus T14V-J1 and its fimbria-deficient mutant strain possessing type 1 fimbriae strongly aggregated with latex beads treated with acidic proline-rich protein 1, basic proline-rich proteins, and proline-rich glycoprotein and its deglycosylated derivative. These type 1+ strains did not aggregate with latex beads treated with other proteins, such as salivary amylase, salivary histidine-rich polypeptides, laminin, type 1 collagen, fibronectin, or C1q. The type 1+ strains also adsorbed well to experimental pellicles formed with acidic proline-rich protein 1, basic proline-rich proteins, and proline-rich glycoprotein and its deglycosylated derivative on hydroxyapatite (HA) surfaces. These interactions were inhibited with immunoglobulins and Fabs specific for type 1 fimbriae. Type 1- actinomyces exhibited feeble adsorption to latex beads or HA treated with any of the aforementioned proteins. Collectively, these data indicate that actinomyces type 1 fimbriae may specifically interact with several proline-rich salivary molecules, forming experimental pellicles on HA or polystyrene surfaces.
Clark
W B
WB
Department of Oral Biology, College of Dentistry, University of Florida, Gainesville 32610.
Beem
J E
JE
Nesbitt
W E
WE
Cisar
J O
JO
Tseng
C C
CC
Levine
M J
MJ
D000190:Actinomyces / Q000187:drug effects / Q000276:immunology / Q000502:physiology*; D000327:Adsorption; D000595:Amino Acid Sequence; D017886:Durapatite; D010861:Fimbriae, Bacterial / Q000502:physiology*; D006882:Hydroxyapatites; D007140:Immunoglobulin Fab Fragments / Q000502:physiology; D007074:Immunoglobulin G / Q000502:physiology; D007841:Latex Fixation Tests / Q000379:methods; D008969:Molecular Sequence Data; D010455:Peptides / Q000494:pharmacology / Q000502:physiology*; D011137:Polystyrenes; D055232:Proline-Rich Protein Domains; D011971:Receptors, Immunologic / Q000502:physiology*; D012471:Salivary Proteins and Peptides / Q000494:pharmacology / Q000502:physiology*
D006882:Hydroxyapatites; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D010455:Peptides; D011137:Polystyrenes; D011971:Receptors, Immunologic; D012471:Salivary Proteins and Peptides; D017886:Durapatite