Abnormal expression of tyrosine hydroxylase-like immunoreactivity in intraocular transplants of rat caudate nucleus.
The purpose of the present study was to examine tyrosine hydroxylase (TH)-like immunoreactivity in single intraocular grafts of caudate nucleus, and in caudate grafts which were co-grafted with substantia nigra. Grafts of caudate obtained from fetal rats (crown-rump length: 15-17 mm) were placed into the anterior chamber of eye of adult female Sprague-Dawley rats, and were allowed to survive from 6 to 20 months. The host rats were then perfused with fixative and the tissue was prepared for immunocytochemistry for TH. In 7 out of 8 surviving caudate grafts, including those containing no substantia nigra, tyrosine TH-like immunoreactive (THLI) cell bodies were present. The results of our study suggest that TH is inappropriately expressed in caudate grafts placed in the anterior chamber of the eye.
Mahalik
T J
TJ
Department of Cellular and Structural Biology, University of Colorado Health Sciences Center, Denver 80262.
Stromberg
I
I
Finger
T E
TE
Olson
L
L
D000818:Animals; D002421:Caudate Nucleus / Q000201:enzymology / Q000637:transplantation*; D005260:Female; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D009799:Ocular Physiological Phenomena; D051381:Rats; D011919:Rats, Inbred Strains; D013378:Substantia Nigra / Q000201:enzymology / Q000637:transplantation; D013997:Time Factors; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*
D014446:Tyrosine 3-Monooxygenase