[Na,K-ATPase activity in the myocardial sarcolemma in ischemia].
The total time-controlled ischemia (up to 45 min) was studied for its effect on the Na,K-ATPase activity, content of nonesterified fatty acids (NEFA) and intensity of lipid peroxidation (LP) in sarcolemmal (SL) preparations and soluble fractions (SF) from the rat and guinea-pig left ventricles. A strong correlation between Na, K-ATPase inhibition and NEFA accumulation was revealed in the SF. On the contrary, changes in the NEFA content and LP level both in SL and SF did not correlate with a decrease in the enzymic activity. Pretreatment with albumin (0.5 mg/ml) induced equally small increase both in the control and in the ischemic SL preparations. It is suggested that the Na,K-ATPase activity during a short period of myocardial ischemia (up to 45 min) may be due to the NEFA accumulation in the cytosolic and/or extracellular space, but not in SL.
Kim
E A
EA
Danilenko
M P
MP
Murzakhmetova
M K
MK
Vashchenko
V I
VI
Esyrev
O V
OV
D000818:Animals; D003327:Coronary Disease / Q000201:enzymology* / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000378:metabolism*; D006168:Guinea Pigs; D015227:Lipid Peroxidation; D008297:Male; D009206:Myocardium / Q000201:enzymology* / Q000378:metabolism; D051381:Rats; D012508:Sarcolemma / Q000201:enzymology* / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000378:metabolism*
D005230:Fatty Acids, Nonesterified; D000254:Sodium-Potassium-Exchanging ATPase
Na,K-ATP-aznaia aktivnost' v sarkolemme miokarda pri ishemii.