Ligand-dependent interaction of the dioxin receptor with target DNA.
Wild type and nuclear transfer deficient mouse hepatoma cell lines were used to study the specific DNA binding of a dioxin inducible factor. This factor interacts with XRE only after dioxin treatment and is absent in receptor mutant containing cells even after treatment. Thus, evidence is provided to substantiate the claim that the dioxin receptor is involved in the specific DNA interaction with dioxin response enhancer elements. It is also shown that the molybdate stabilised dioxin-receptor interacts with hsp90 suggesting that, in similarity to the glucocorticoid receptor, the dioxin receptor is kept in a non-transformed state in the absence of ligand.
Cuthill
S
S
Department of Medical Nutrition, Karolinska Institute, Huddinge University Hospital, Sweden.
Hapgood
J
J
Denis
M
M
Wilhelmsson
A
A
Mason
G
G
Nemoto
T
T
Widmark
E
E
Poellinger
L
L
Gustafsson
J A
JA
D000818:Animals; D001483:Base Sequence; D001667:Binding, Competitive; D002460:Cell Line; D004273:DNA, Neoplasm / Q000378:metabolism*; D004147:Dioxins / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D015345:Oligonucleotide Probes; D000072317:Polychlorinated Dibenzodioxins / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D018336:Receptors, Aryl Hydrocarbon; D011955:Receptors, Drug / Q000235:genetics / Q000378:metabolism*
D004273:DNA, Neoplasm; D004147:Dioxins; D015345:Oligonucleotide Probes; D000072317:Polychlorinated Dibenzodioxins; D018336:Receptors, Aryl Hydrocarbon; D011955:Receptors, Drug