Bronchoalveolar leukocyte response in experimental silicosis: modulation by a soluble aluminum compound.
The biological properties of quartz have been related to its surface reactivity. We have addressed the role of particle surface reactivity in mediating the biological activity of quartz in mixed dusts, by treating the quartz with aluminum lactate. Intratracheal instillation of untreated quartz in rats caused a rapid, sustained alveolitis and bronchoalveolar leukocytes obtained from these animals had enhanced activity in degrading fibronectin, but reduced ability to mount a respiratory burst. Quartz pretreated with aluminum elicited a markedly reduced inflammatory response; the reduced activity of the treated quartz was also reflected in the attenuated change in the key functional parameters, oxidant production and proteolysis of fibronectin. Late intratracheal dosing with aluminum after the quartz-induced alveolitis was well established reduced the inflammatory response and abrogated the effect of quartz on the respiratory burst, but did not alter fibronectin degradation by the leukocytes. Aluminum did not affect the inflammatory response to Corynebacterium parvum and thus the effect was on the quartz particles and not on the inflammatory leukocytes. These findings have implications for the likely pulmonary responses to mixed dusts containing quartz and aluminum silicate clays.
Brown
G M
GM
Institute of Occupational Medicine, Roxburgh Place, Edinburgh, United Kingdom.
Donaldson
K
K
Brown
D M
DM
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D005260:Female; D007773:Lactates / Q000494:pharmacology*; D019344:Lactic Acid; D007958:Leukocyte Count / Q000187:drug effects; D007962:Leukocytes / Q000187:drug effects / Q000502:physiology*; D009504:Neutrophils / Q000187:drug effects; D010084:Oxidation-Reduction; D011425:Propionibacterium acnes / Q000276:immunology; D011506:Proteins / Q000378:metabolism; D011791:Quartz; D051381:Rats; D012822:Silicon Dioxide; D012829:Silicosis / Q000276:immunology / Q000473:pathology*; D013499:Surface Properties
D007773:Lactates; D011506:Proteins; D011791:Quartz; D019344:Lactic Acid; D012822:Silicon Dioxide