title: Calpain proteolysis of free and bound forms of calponin, a troponin T-like protein in smooth muscle.
abstract: Calponin, a novel homologue of troponin T, purified from chicken gizzard was found to be one of the most susceptible proteins among smooth muscle contraction-associated proteins to hydrolysis by calpain I purified from human red blood cells. The high susceptibility of calponin was comparable to that reported for troponin T. The rate of degradation of calponin, unlike caldesmon and myosin light chain kinase, was accelerated when bound to calmodulin. When calponin existed as a bound form in both reconstituted actin filament and native thin filament, the rate of proteolysis was markedly retarded, indicating close association of calponin with actin filament. These observations are compatible with the view that calponin is an integral part of the actin-linked contractile machinery in smooth muscle.
authors.affiliation: Department of Clinical Science, Faculty of Medicine, Kyoto University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Tsunekawa
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
authors.forename: M
authors.initials: M
authors.lastname: Abe
authors.forename: K
authors.initials: K
authors.lastname: Hiwada
authors.forename: K
authors.initials: K
authors.lastname: Ozawa
authors.forename: T
authors.initials: T
authors.lastname: Murachi
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002147:Calmodulin / Q000494:pharmacology; D002154:Calpain / Q000494:pharmacology*; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D008840:Microfilament Proteins; D009124:Muscle Proteins / Q000378:metabolism*; D009130:Muscle, Smooth / Q000378:metabolism*; D014336:Troponin / Q000378:metabolism*; D000096985:Calponins
chemical_list: D002135:Calcium-Binding Proteins; D002147:Calmodulin; D002154:Calpain; D008840:Microfilament Proteins; D009124:Muscle Proteins; D014336:Troponin; D000096985:Calponins