title: Evidence that the production of acetate in rat hepatocytes is a predominantly cytoplasmic process.
abstract: By using [1-14C]butyrate, the fluxes of butyrate to acetate and fatty acids were measured in rat hepatocytes. Both fluxes were inhibited to a similar extent by (-)-hydroxycitrate, with no significant effect on butyrate uptake. These results indicate that acetate formation takes place in the cytoplasm, presumably via ATP-stimulated acetyl-CoA hydrolase. Since acetate formation occurred despite a net uptake of acetate, the results are also consistent with the operation of a substrate cycle between acetate and acetyl-CoA, recently proposed by other workers, and suggest that this cycle is cytoplasmic.
authors.affiliation: Rowett Research Institute, Bucksburn, Aberdeen, U.K.
authors.forename: B
authors.initials: B
authors.lastname: Crabtree
authors.forename: M J
authors.initials: MJ
authors.lastname: Souter
authors.forename: S E
authors.initials: SE
authors.lastname: Anderson
mesh_terms: D000085:Acetates / Q000378:metabolism*; D000105:Acetyl Coenzyme A / Q000378:metabolism; D000104:Acetyl-CoA Hydrolase / Q000378:metabolism; D000818:Animals; D002087:Butyrates / Q000378:metabolism; D020148:Butyric Acid; D002951:Citrates / Q000494:pharmacology; D003593:Cytoplasm / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D006868:Hydrolysis; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D051381:Rats
chemical_list: D000085:Acetates; D002087:Butyrates; D002951:Citrates; D005227:Fatty Acids; D020148:Butyric Acid; D000105:Acetyl Coenzyme A; C007999:hydroxycitric acid; D000104:Acetyl-CoA Hydrolase