title: A spectrophotometric procedure for measuring oxoglutarate and determining aminotransferase activities using nicotinamide adenine dinucleotide phosphate-linked glutamate dehydrogenase from algae.
abstract: A new spectrophotometric procedure is described for determining glutamate-dependent activities of aspartate aminotransferase, alanine aminotransferase, and ornithine aminotransferase with NADPH-linked glutamate dehydrogenase (GDH) from nitrate-grown Stichococcus bacillaris. The algal NADPH-GDH is highly specific for oxoglutarate and can catalyze the reduction of this keto acid in the presence of high glutamate concentrations, and thus is suitable for the measurement of oxoglutarate produced in glutamate-dependent amino-transferase reactions. The alga produces large amounts of NADPH-GDH which can be adequately purified in a few simple steps. The purified enzyme can be stored at 4 degrees C for several weeks without any detectable loss of activity. The algal NADPH-GDH can also be used for the estimation of small amounts of oxoglutarate in aqueous extracts.
authors.affiliation: Department of Botany, University of Toronto, Ontario, Canada.
authors.forename: I
authors.initials: I
authors.lastname: Ahmad
authors.forename: J A
authors.initials: JA
authors.lastname: Hellebust
mesh_terms: D000818:Animals; D000460:Chlorophyta / Q000201:enzymology*; D005969:Glutamate Dehydrogenase / Q000302:isolation & purification / Q000378:metabolism*; D018086:Glutamate Dehydrogenase (NADP+); D007656:Ketoglutaric Acids / Q000032:analysis*; D010944:Plants; D013053:Spectrophotometry; D013552:Swine; D000637:Transaminases / Q000378:metabolism*
chemical_list: D007656:Ketoglutaric Acids; D005969:Glutamate Dehydrogenase; D018086:Glutamate Dehydrogenase (NADP+); D000637:Transaminases