Detection of bovine herpesvirus-1-specific IgM using a capture enzyme immune assay with isotype-specific monoclonal antibodies.
The detection of virus-specific immunoglobulin M (IgM) antibodies in acute-phase serum samples offers the possibility of making an accurate and rapid serologic diagnosis. We have developed a solid-phase capture assay that uses murine monoclonal antibodies specific for bovine IgM to separate the whole IgM fraction of a bovine serum sample. The IgM specific for bovine herpesvirus-1 (BHV-1) is then detected by the addition of viral antigen, which in turn is detected by BHV-1-specific monoclonal antibodies conjugated to horseradish peroxidase. A BHV-1 IgM antibody response was detected during the early postinfection period (7-40 days PI). Bovine herpesvirus-1 IgM antibody was not detected in sera taken from 3 animals following dexamethasone-induced viral reactivation. This method compares favorably with viral isolation, antigen detection in the clinical samples, and paired serology in the diagnosis of BHV-1 infection at a herd level.
Osorio
F
F
Veterinary Diagnostic Center, University of Nebraska-Lincoln 68583-0905.
Srikumaran
S
S
Rhodes
M
M
Christensen
D
D
Srikumaran
P
P
D000208:Acute Disease; D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000097:blood*; D000918:Antibody Specificity; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D007242:Herpesvirus 1, Bovine / Q000276:immunology*; D007124:Immunoenzyme Techniques; D007075:Immunoglobulin M / Q000032:analysis*; D007241:Infectious Bovine Rhinotracheitis / Q000175:diagnosis*; D011237:Predictive Value of Tests; D011863:Radioimmunoassay; D012008:Recurrence
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D007075:Immunoglobulin M