An improved ELISA and serum neutralisation test for the detection of turkey rhinotracheitis virus antibodies.
The development of a double-well ELISA test for the detection of turkey rhinotracheitis (TRT) virus antibody in turkey and chicken sera, utilising a streptavidin-biotin detection protocol is described. This test was compared with a conventional ELISA, which detects antibody using a peroxidase-labelled anti-turkey IgG. The double-well streptavidin-biotin ELISA was more sensitive than the conventional ELISA, and, due to the consistently low background values obtained, was further modified to a single-well ELISA test. Antibody titres obtained using the single-well streptavidin-biotin ELISA were similar to those obtained using the conventional ELISA. A microtitre serum neutralisation test for TRT virus read using an indirect immunoperoxidase (IIP) detection protocol after 48 h was also developed and compared to the standard neutralisation test employing a 7 day incubation and read by CPE. The specificity of the single-well streptavidin-biotin ELISA was confirmed using the rapid (IIP) serum neutralisation test.
O'Loan
C J
CJ
Veterinary Research Laboratories, Belfast, Ireland.
Allan
G
G
Baxter-Jones
C
C
McNulty
M S
MS
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D001426:Bacterial Proteins; D001710:Biotin; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000276:immunology; D009500:Neutralization Tests; D011237:Predictive Value of Tests; D012229:Rhinovirus / Q000276:immunology*; D019809:Streptavidin; D014422:Turkeys / Q000382:microbiology*
D000914:Antibodies, Viral; D001426:Bacterial Proteins; D007106:Immune Sera; D007074:Immunoglobulin G; D001710:Biotin; D019809:Streptavidin