Immunocytochemical demonstration of cytochrome c oxidase as a marker for renal oncocytes and oncocytomas.
Using an antibody raised against the mitochondrial enzyme cytochrome c oxidase (EC 1.9.3.1), it was possible to visualize microoncocytomas, oncocytic tubules and even single oncocytes in the renal cortex of rats treated with N-nitrosomorpholine, and to localize the lesions to the collecting duct of the nephron. In human specimens, the antibody also stained oncocytomas and oncocytic tubules in the surrounding macroscopically unaffected cortex. Thus, this antibody may be used for detection as well as for investigation of the development of oncocytic lesions in various species.
Mayer
D
D
Institut für Experimentelle Pathologie, Deutsches Krebsforschungszentrum, Heidelberg, FRG.
Weber
E
E
Kadenbach
B
B
Bannasch
P
P
D000818:Animals; D015415:Biomarkers; D003576:Electron Transport Complex IV / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D007202:Indicators and Reagents; D007680:Kidney Neoplasms / Q000473:pathology / Q000503:physiopathology*; D007684:Kidney Tubules / Q000201:enzymology / Q000473:pathology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D015415:Biomarkers; D007202:Indicators and Reagents; D003576:Electron Transport Complex IV