Complete coding sequence of wheat phosphoribulokinase: developmental and light-dependent expression of the mRNA.
We have isolated and sequenced a cDNA clone which contains the entire coding sequence of the precursor to a subunit of wheat phosphoribulokinase (PRKase). (The enzyme is a homodimer). The cDNA contains 1533 bp and has an open reading frame of 1212 nucleotides. This encodes a protein with an amino-terminal transit sequence of 53 amino acids, while the part that forms the mature protein contains 351 amino acids and has a molecular weight of 39,200 daltons. A comparison of the wheat amino acid sequence with that already known for the mature protein of spinach reveals that there are identical residues in 86% of the positions but their transit peptides differ substantially from one another. The mature wheat and spinach proteins are identical in a segment of over 50 amino acids near the amino-terminus which is the region believed to be involved in ATP binding and in regulation by light of the catalytic activity of the enzyme. We further demonstrate that the expression of PRKase mRNA in wheat leaves is regulated in a developmental, tissue-specific and light dependent manner. We also show that the light-induced increase in the steady-state levels of this mRNA is dependent on the developmental stage of the leaf.
Raines
C A
CA
Institute of Plant Science Research (Cambridge Laboratory), UK.
Longstaff
M
M
Lloyd
J C
JC
Dyer
T A
TA
D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA; D005784:Gene Amplification; D005786:Gene Expression Regulation; D015151:Immunoblotting; D008029:Lighting; D008969:Molecular Sequence Data; D010770:Phosphotransferases / Q000235:genetics*; D017853:Phosphotransferases (Alcohol Group Acceptor); D012333:RNA, Messenger / Q000096:biosynthesis*; D014908:Triticum / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development
D012333:RNA, Messenger; D004247:DNA; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C022390:phosphoribulokinase