title: NADPH-dependent H2O2 generation catalyzed by thyroid plasma membranes. Studies with electron scavengers.
abstract: Hog thyroid plasma membrane preparations containing a Ca2+-regulated NADPH-dependent H2O2-generating system were studied. The Ca2+-dependent reductase activities of ferricytochrome c, 2,6-dichloroindophenol, nitroblue tetrazolium, and potassium ferricyanide were tested and the effect of these scavengers on H2O2 formation, NADPH oxidation and O2 consumption were measured, with the following results. 1. Thyroid plasma membrane Ca2+-independent cytochrome c reduction was not catalyzed by the NADPH-dependent H2O2-generating system. This activity was superoxide-dismutase-insensitive. 2. Of the three other electron scavengers tested, only K3Fe(CN)6 was clearly, but partially reduced in a Ca2+-dependent manner. 3. Though the NADPH-dependent reduction of nitroblue tetrazolium was very low and superoxide-dismutase-insensitive, nitroblue tetrazolium inhibited O2 consumption, H2O2 formation and NADPH oxidation, indicating that nitroblue tetrazolium inhibits the H2O2-generating system. We conclude that the thyroid plasma membrane H2O2-generating system does not or liberate O2- and that Ca2+ controls the first step (NADPH oxidation) of the H2O2-generating system.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, Unité de Recherches sur la Glande Thyroide et la Régulation Hormonale, Le-Kremlin-Bicêtre, France.
authors.forename: C
authors.initials: C
authors.lastname: Dupuy
authors.forename: J
authors.initials: J
authors.lastname: Kaniewski
authors.forename: D
authors.initials: D
authors.lastname: Dème
authors.forename: J
authors.initials: J
authors.lastname: Pommier
authors.forename: A
authors.initials: A
authors.lastname: Virion
mesh_terms: D015086:2,6-Dichloroindophenol / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism*; D002793:Cholic Acids / Q000494:pharmacology; D003574:Cytochrome c Group / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004579:Electron Transport; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008565:Membrane Proteins / Q000494:pharmacology*; D009249:NADP / Q000494:pharmacology*; D009580:Nitroblue Tetrazolium / Q000494:pharmacology; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D013552:Swine; D013961:Thyroid Gland / Q000378:metabolism*
chemical_list: D002793:Cholic Acids; D003574:Cytochrome c Group; D008565:Membrane Proteins; D009580:Nitroblue Tetrazolium; D009249:NADP; D006861:Hydrogen Peroxide; D015086:2,6-Dichloroindophenol; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate; D002118:Calcium