Characterization of SV40-transformed human cells by immunofluorescence and fluorescent in situ hybridization techniques.
We have performed immunofluorescent and fluorescent in situ hybridization studies in order to better clarify the integration of SV40 DNA in human fibroblast cell lines. Most of the cells were T-antigen positive by immunocytochemical studies, while in all the cells we detected the integrated viral DNA by in situ hybridization. Both techniques are easy and useful to perform but the molecular genetic method gives a more specific signal with the possibility of localizing molecular hybrids in the nucleus and in the cytoplasm of the transformed cells.
Prelle
A
A
Istituto di Clinica Neurologica Centro Dino Ferrari, Università di Milano, Italy.
Moggio
M
M
Fagiolari
G
G
Scarlato
G
G
Miranda
A
A
Bonilla
E
E
D000952:Antigens, Polyomavirus Transforming / Q000032:analysis / Q000276:immunology; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D005347:Fibroblasts / Q000032:analysis* / Q000276:immunology / Q000473:pathology; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D009693:Nucleic Acid Hybridization; D013539:Simian virus 40 / Q000235:genetics*
D000952:Antigens, Polyomavirus Transforming; D015342:DNA Probes; D004279:DNA, Viral