title: Cyclin synthesis drives the early embryonic cell cycle.
abstract: We have produced extracts of frog eggs that can perform multiple cell cycles in vitro. Destruction of the endogenous messenger RNA arrests the extracts in interphase. The addition of exogenous cyclin mRNA is sufficient to produce multiple cell cycles. The newly synthesized cyclin protein accumulates during each interphase and is degraded at the end of each mitosis.
authors.affiliation: Department of Biochemistry and Biophysics, School of Medicine, University of California, San Francisco 94143-0448.
authors.forename: A W
authors.initials: AW
authors.lastname: Murray
authors.forename: M W
authors.initials: MW
authors.lastname: Kirschner
mesh_terms: D000818:Animals; D002453:Cell Cycle; D002467:Cell Nucleus / Q000648:ultrastructure; D004625:Embryo, Nonmammalian / Q000166:cytology* / Q000502:physiology; D005260:Female; D006133:Growth Substances / Q000502:physiology; D007399:Interphase; D016200:Maturation-Promoting Factor; D008677:Metaphase; D008938:Mitosis; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger / Q000235:genetics; D014981:Xenopus
chemical_list: D006133:Growth Substances; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger; D016200:Maturation-Promoting Factor