[Subunit components of transmissive gastroenteritis virus of swine: preparing purified substances and assessing antigenic properties].
Two different methods were used to prepare subunit components of transmissible gastroenteritis (TGE) virus. The ultrastructure of the peplomer glycoprotein E2 of TGE virus was studied. The presence of detergent NP-40 was shown to prevent the formation of this glycoprotein aggregates. In dialysed preparations, the peplomers are aggregated to form multimer structures, the so-called "rosettes". The orientation of the peplomers in the "rosettes" and in the whole virion was identical. The antigenic activity of viral subunits was studied in guinea pigs. Preparations of isolated peplomers were shown to induce enhanced production of virus-neutralizing antibodies whose mean titre exceeded that in the group of animals immunized with whole virion-based preparations.
Rukhadze
G G
GG
Shcheglova
E Iu
EIu
Aliper
T I
TI
Khaustov
V I
VI
Sergeev
V A
VA
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000917:Antibody Formation; D000956:Antigens, Viral / Q000276:immunology* / Q000302:isolation & purification; D003332:Coronaviridae / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D005761:Gastroenteritis, Transmissible, of Swine / Q000276:immunology* / Q000517:prevention & control; D006023:Glycoproteins / Q000302:isolation & purification / Q000648:ultrastructure; D006168:Guinea Pigs; D007114:Immunization; D008854:Microscopy, Electron; D012397:Rosette Formation; D013552:Swine; D005760:Transmissible gastroenteritis virus / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D006023:Glycoproteins
Sub''edinichnye komponenty virusa transmissivnogo gastroénterita svineĭ: poluchenie ochishchennykh preparatov i otsenka antigennykh svoĭstv.