title: The effect of short-wavelength ultraviolet light on antigens, lectin receptors and the ultrastructure of Crithidia fasciculata.
abstract: Crithidia fasciculata is an important trypanosomatid parasite commonly affecting insects and is used extensively as a model for the study of the biochemistry, ultrastructure and organization of the kDNA network of trypanosomatids. The present study describes the evolution of UV-induced morphological changes detectable by transmission electron microscopy in Crithidia fasciculata. Although only rare and minor changes in kinetoplast DNA were demonstrable 7 h after UV irradiation, alterations of this organelle were present in almost all flagellates observed 24 h and 48 h after irradiation. Other cell structures were apparently undamaged. Ultrastructural changes in kDNA did not correspond to changes in antigenicity of protein bands in western blotting against serum from Chagas' disease patients or in the presence of 3 different lectin receptors on the surface of the parasite.
authors.affiliation: Departamento de Biologia Geral, Universidade Federal de Pernambuco, Recife, Brasil.
authors.forename: M A
authors.initials: MA
authors.lastname: Santos
authors.forename: A M
authors.initials: AM
authors.lastname: Barros
authors.forename: P P
authors.initials: PP
authors.lastname: Andrade
authors.forename: I P
authors.initials: IP
authors.lastname: Padovan
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000528:radiation effects*; D003421:Crithidia / Q000276:immunology / Q000648:ultrastructure*; D004270:DNA, Circular / Q000528:radiation effects*; D018105:DNA, Kinetoplast; D011975:Receptors, Mitogen / Q000528:radiation effects*; D014466:Ultraviolet Rays
chemical_list: D000953:Antigens, Protozoan; D004270:DNA, Circular; D018105:DNA, Kinetoplast; D011975:Receptors, Mitogen