Effect of a Campylobacter pylori protein on acid secretion by parietal cells.
The uptake of [14C]-aminopyrine by rabbit gastric epithelial cells was used as an indirect assay for acid secretion from parietal cells. Campylobacter pylori strains, isolated from the stomachs of 3 patients with chronic gastritis, duodenal ulcer, and near-normal mucosa, respectively, inhibited acid secretion as effectively as 10(-4) mol/l cimetidine. The inhibition occurred with whole organisms and with sonicates. Preliminary characterisation suggested that the inhibition was due to a nondialysable protein. The inhibitor was not toxic to gastric epithelial cells. This bacterial product may explain the hypochlorhydria seen in man acutely infected with C pylori.
Cave
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Evans Memorial Department of Clinical Research, Boston University Medical Center, Massachusetts.
Vargas
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D000632:Aminopyrine; D000818:Animals; D001426:Bacterial Proteins / Q000494:pharmacology*; D001431:Bacteriological Techniques; D002167:Campylobacter / Q000254:growth & development; D002250:Carbon Radioisotopes; D002927:Cimetidine / Q000494:pharmacology; D005744:Gastric Acid / Q000378:metabolism*; D008297:Male; D010295:Parietal Cells, Gastric / Q000187:drug effects*; D011817:Rabbits
D001426:Bacterial Proteins; D002250:Carbon Radioisotopes; D000632:Aminopyrine; D002927:Cimetidine