Binding of connectin to myosin filaments.
Binding of native connectin (2,100 kDa fragment of alpha-connectin) to myosin filaments was investigated using a sedimentation technique and densitometric estimations of the separated proteins. In the presence of 60 mM KCl and 5 mM phosphate buffer, pH 7.0, as much as 1.5 mol of connectin was bound to 1 mol of myosin, suggesting that some 150 connectin filaments bound to a single myosin filament of approximately 0.5 micron in length. This value was much more than the ratio found in muscle (12:1). It appeared that C protein did not affect the binding of connectin to myosin filaments.
Murayama
T
T
Department of Biology, Faculty of Science, Chiba University.
Nakauchi
Y
Y
Kimura
S
S
Maruyama
K
K
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D002645:Chickens; D064211:Connectin; D003720:Densitometry; D008854:Microscopy, Electron; D009124:Muscle Proteins / Q000378:metabolism*; D009218:Myosins / Q000378:metabolism*; D011189:Potassium Chloride; D011485:Protein Binding; D011494:Protein Kinases; D011817:Rabbits
D002352:Carrier Proteins; D064211:Connectin; D009124:Muscle Proteins; C020723:citrate-binding transport protein; D011189:Potassium Chloride; D011494:Protein Kinases; D009218:Myosins