Rapid culture confirmation of adenovirus isolates using the adenoclone enzyme immunoassay (EIA) test kit.
The adenoclone enzyme immunoassay (EIA) test kit (Cambridge BioScience Corp., Worcester, MA) was evaluated using 43 adenovirus and 53 nonadenovirus (i.e., polio-, echo-, coxsackievirus types A,B, parainfluenza virus, and herpes simplex virus) isolates obtained during routine clinical diagnostic screening procedures. Forty-two of 43 confirmed adenovirus isolates were reactive upon testing by the adenoclone test kit. None of the 53 nonadenovirus isolates, including seven difficult-to-cultivate group A coxsackievirus isolates, cross-reacted with the EIA kit. The adenoclone EIA kit is an appropriate supplement to the regimen of clinical diagnostic procedures for the culture confirmation of adenovirus.
Lipson
S M
SM
Department of Pathology and Laboratories, Nassau County Medical Center, East Meadow, New York 11554.
D000256:Adenoviridae / Q000302:isolation & purification*; D000257:Adenoviridae Infections / Q000175:diagnosis; D005069:Evaluation Studies as Topic; D006801:Humans; D007124:Immunoenzyme Techniques