Vitamin D3 administration increases the membrane fluidity of intestinal mitochondria.
The fluorescence anisotropy in the mitochondria from vitamin D-treated chicks is significantly lower than that from the vitamin D-deficient animals with the inner core probe DPH. Surface membrane fluidity, measured with the probe TMA-DPH, shows no differences between the organelles of both groups. The fluorescence studies performed in mitochondrial subfractions revealed that cholecalciferol treatment induces a decrease of lipid order parameter S (DPH) in the mitochondrial inner membrane. These results pose the question of whether vitamin D3 participates in the regulation of physiological function of the intestinal mitochondria through changes in the physical properties of the membranes.
Tolosa de Talamoni
N
N
Cátedra de Química Biológica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba.
Morero
R
R
Cañas
F
F
D000818:Animals; D002645:Chickens; D002762:Cholecalciferol / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D005454:Fluorescence Polarization; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects*; D008560:Membrane Fluidity / Q000187:drug effects*; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism
D002762:Cholecalciferol