title: Sequential changes in the harderian and exorbital lacrimal glands in Wistar rats infected with sialodacryoadenitis virus.
abstract: A sequential light and electron microscopic study of the exorbital and Harderian lacrimal glands was done on 2.5- to 15-month-old Wistar rats exposed to sialodacryoadenitis (SDA) virus. Typical coronaviral particles were readily demonstrated in cytoplasmic vesicles of Harderian and exorbital glands examined at 6 days post-inoculation. Lesions were seen in a relatively high percentage of lacrimal glands in infected animals of all ages, with no obvious age-related variations in the incidence and extent of changes. Lesions frequently persisted for a longer interval post-exposure in lacrimal glands than in salivary glands. The persistence of lesions commonly seen in Harderian glands was attributed, at least in part, to the cytotoxic effects of porphyrin-containing secretions released during the acute necrotizing stages of the disease. The persistence of lesions in some lacrimal glands indicates that they are useful tissues for microscopic examination for the retrospective provisional diagnosis of SDA. Persistent lesions also indicate that normal functions of these glands may be compromised for up to several weeks following outbreaks of SDA.
authors.affiliation: Department of Pathology, Ontario Veterinary College, University of Guelph, Canada.
authors.forename: D H
authors.initials: DH
authors.lastname: Percy
authors.forename: Z W
authors.initials: ZW
authors.lastname: Wojcinski
authors.forename: M K
authors.initials: MK
authors.lastname: Schunk
mesh_terms: D000367:Age Factors; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D003332:Coronaviridae / Q000276:immunology; D003333:Coronaviridae Infections / Q000473:pathology / Q000662:veterinary*; D003607:Dacryocystitis / Q000473:pathology / Q000662:veterinary*; D005260:Female; D005455:Fluorescent Antibody Technique; D006243:Harderian Gland / Q000473:pathology / Q000648:ultrastructure; D007765:Lacrimal Apparatus / Q000473:pathology* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains; D012376:Rodent Diseases / Q000473:pathology*; D012469:Salivary Glands / Q000473:pathology / Q000648:ultrastructure; D013047:Specific Pathogen-Free Organisms
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral