title: HSV hepatitis in the mouse: a light and electron microscopic study with immunohistology and in situ hybridization.
abstract: In order to characterize better the morphology and immune response in acute necrotizing HSV infection, murine HSV hepatitis was examined. BALB/c mice were inoculated intraperitoneally with 10(6) plaque-forming units (PFU) of HSV-1 (Lenette) and HSV-2 (D316). In both groups half the animals were pretreated with silica particles to block macrophage function. Up to 6 days after infection four mice from each group were sacrificed at daily intervals and the livers were examined by light and electron microscopy, immunohistology, in situ hybridization, combined immunohistology/in situ hybridization and titration of viral PFU. HSV-2 infected mice developed severe necrotizing hepatitis with persistence of HSV in the liver tissue until the end of the study. HSV-1 infected mice rapidly eliminated the virus and revealed only small necrotic foci. Early phase alterations and necrotic phase lesions were distinguished and characterized and morphologic evidence of a direct cytopathic effect of HSV was detected. A specific immune reaction in late stages appeared to be mediated by T4-positive T-lymphocytes. In situ hybridization and immunohistochemistry showed a close correlation with virus titration and were valuable in characterizing early phases and in the assessment of prognosis and differential diagnosis.
authors.affiliation: Pathologisches Institut, Johannes Gutenberg-Universität, Mainz, Federal Republic of Germany.
authors.forename: P
authors.initials: P
authors.lastname: Schirmacher
authors.forename: M
authors.initials: M
authors.lastname: Wörsdörfer
authors.forename: K
authors.initials: K
authors.lastname: Lübbe
authors.forename: D
authors.initials: D
authors.lastname: Falke
authors.forename: W
authors.initials: W
authors.lastname: Thoenes
authors.forename: H P
authors.initials: HP
authors.lastname: Dienes
mesh_terms: D000818:Animals; D004247:DNA / Q000235:genetics; D005260:Female; D006524:Hepatitis, Viral, Animal / Q000209:etiology / Q000473:pathology*; D006561:Herpes Simplex / Q000150:complications / Q000473:pathology*; D007150:Immunohistochemistry; D007958:Leukocyte Count; D008099:Liver / Q000648:ultrastructure; D008214:Lymphocytes; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D009693:Nucleic Acid Hybridization; D014779:Virus Replication
chemical_list: D004247:DNA