Negative enrichment of bovine T lymphocytes with monoclonal antibodies and magnetic microspheres.
A highly enriched population of bovine T lymphocytes was produced from peripheral blood leukocytes following the depletion of monoclonal antibody-labelled B lymphocytes and monocytes with magnetic microspheres. This negative-enrichment protocol was simple, rapid, and specific. Also, it had a high recovery efficiency and was consistently reproducible. The enriched T lymphocytes proliferated in response to recombinant bovine interleukin 2 and, following the addition of monocytes, to concanavalin A. This methodology made it possible to determine the proliferative responses of peripheral blood lymphocytes utilizing a constant number of T lymphocytes within each assay. In this way, the in vitro T lymphocyte responses were determined independent of changes in the number of responder cells within peripheral blood.
Griebel
P J
PJ
Department of Veterinary Microbiology, University of Saskatchewan, Saskatoon, Canada.
Gee
A P
AP
Qualtiere
L
L
Lawman
M J
MJ
Babiuk
L A
LA
D000818:Animals; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000276:immunology; D002417:Cattle; D002469:Cell Separation / Q000379:methods*; D003208:Concanavalin A / Q000494:pharmacology; D005434:Flow Cytometry; D007242:Herpesvirus 1, Bovine / Q000276:immunology; D007241:Infectious Bovine Rhinotracheitis / Q000276:immunology; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation; D008280:Magnetics; D008863:Microspheres; D009000:Monocytes / Q000276:immunology; D011994:Recombinant Proteins; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D007376:Interleukin-2; D011994:Recombinant Proteins; D003208:Concanavalin A