Performing nucleic acid reactions using predispensed lyophilized reaction mixtures.
A system is described in which manipulations of nucleic acids are performed in wells containing predispensed lyophilized reaction mixtures requiring addition of only nucleic acid. This allows increased reproducibility for single-step reactions (e.g., restrictions and ligations), as well as improved productivity for complex reactions (e.g., sequencing). Enzymes, co-factors, nucleotides and buffers can be dried and stored at room temperature without loss of essential function. When used for DNA sequencing, hundreds of templates a day can be sequenced with the potential to determine megabase amounts of sequence per week.
Ortlepp
S A
SA
Surgical Unit, London Hospital Medical College, University of London, Whitechapel, U.K.
McKay
I A
IA
D001483:Base Sequence; D001709:Biotechnology; D004247:DNA; D011088:DNA Ligases; D004262:DNA Restriction Enzymes; D005612:Freeze Drying; D007202:Indicators and Reagents; D009696:Nucleic Acids
D007202:Indicators and Reagents; D009696:Nucleic Acids; D004247:DNA; D004262:DNA Restriction Enzymes; D011088:DNA Ligases