Prevention of myocardial reperfusion injury with free radical scavengers. An experimental study.
The changes in endogenous superoxide dismutase (ESOD) during myocardial ischemia and reperfusion and the efficacy of oxygen free radical scavengers in myocardial protection were investigated in an isolated heart model connected with the recirculating nonpulsatile perfusion circuit. Subjected to a 2-hour period of global ischemia (27 C), the heart was reperfused with 37 C oxygen diluted auto-blood for 60 minutes. Superoxide dismutase plus catalase was added into the cardioplegic solution and reperfusates. ESOD activity was measured by pyrogallol method. The results of the experiment showed that ESOD activity after ischemia and reperfusion was decreased and the addition of oxygen free radical scavengers (SOD and CAT) to the cardioplegic solution and the reperfusates greatly reduced the leakage of myocardial enzymes, coronary vascular resistance, and the ultrastructural damages of the myocardium. These results suggest that the use of SOD and CAT may inhibit myocardial reperfusion injury by scavenging oxygen-derived free radicals.
Hou
D
D
Liu
W Y
WY
Fang
F Z
FZ
Mo
J
J
Sun
S F
SF
Shi
W R
WR
Ye
J M
JM
Guan
Y F
YF
Liu
J
J
D000818:Animals; D002374:Catalase / Q000494:pharmacology / Q000627:therapeutic use*; D003326:Coronary Circulation; D005260:Female; D005609:Free Radicals; D066298:In Vitro Techniques; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D009206:Myocardium / Q000378:metabolism / Q000648:ultrastructure; D011817:Rabbits; D013482:Superoxide Dismutase / Q000494:pharmacology / Q000627:therapeutic use*; D013481:Superoxides; D014655:Vascular Resistance
D005609:Free Radicals; D013481:Superoxides; D002374:Catalase; D013482:Superoxide Dismutase