title: Effect of muramyl peptides and tumor necrosis factor on oxidative responses of human blood phagocytes.
abstract: Adjuvant muramyl dipeptides enhanced the intracellular oxidative burst induced by phorbol myristate acetate in purified human polymorphonuclear (PMN) cells and in monocytes. A stronger priming effect was obtained when muramyl dipeptide was conjugated to a protein carrier. Recombinant human tumor necrosis factor (TNF) did not modify the level of intracellular NBT reduction in PMA-stimulated PMN, although it slightly increased the secretion of superoxide anion. In contrast, TNF enhanced the intracellular oxidative burst of monocytes even at the concentration of 10 pg/ml. In human PMN only, the combination of TNF and muramyl dipeptide induced a higher oxidative response than each stimulant alone.
authors.affiliation: Laboratory of Immunopharmacology, Centre de Recherches Biomédicales des Cordeliers, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Jupin
authors.forename: M
authors.initials: M
authors.lastname: Parant
authors.forename: L
authors.initials: L
authors.lastname: Chedid
mesh_terms: D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000494:pharmacology*; D003574:Cytochrome c Group / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000494:pharmacology; D009000:Monocytes / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D010084:Oxidation-Reduction; D010586:Phagocytes / Q000187:drug effects* / Q000378:metabolism; D011994:Recombinant Proteins; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D003574:Cytochrome c Group; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D009580:Nitroblue Tetrazolium; D000119:Acetylmuramyl-Alanyl-Isoglutamine; D013755:Tetradecanoylphorbol Acetate