Evidence that v-src and v-fps gene products use a protein kinase C-mediated pathway to induce expression of a transformation-related gene.
Induction of the transformation-related gene 9E3 by the v-src and v-fps gene products (v-Src and v-Fps) is blocked in chicken embryo fibroblasts depleted of protein kinase C (PKC). PKC agonists induce 9E3 gene expression. Protein kinase inhibitors block v-Src- and v-Fps-induced 9E3 gene expression with the same dose-response curves seen for PKC agonist-induced 9E3 gene expression. These data suggest that v-Src and v-Fps use a PKC-mediated signal-transduction pathway to induce expression of the transformation-related 9E3 gene. Consistent with this hypothesis, we find that both v-Src and v-Fps rapidly induce phosphorylation of a 67-kDa PKC substrate.
Spangler
R
R
Institute for Biomolecular Structure and Function, Hunter College, City University of New York, NY 10021.
Joseph
C
C
Qureshi
S A
SA
Berg
K L
KL
Foster
D A
DA
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002471:Cell Transformation, Neoplastic; D002642:Chick Embryo; D015683:Gene Products, gag; D007546:Isoquinolines / Q000494:pharmacology; D007700:Kinetics; D015688:Oncogene Protein pp60(v-src); D009857:Oncogenes; D010703:Phorbol Esters / Q000494:pharmacology; D010766:Phosphorylation; D010879:Piperazines / Q000494:pharmacology; D011493:Protein Kinase C / Q000502:physiology*; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics; D012191:Retroviridae Proteins / Q000502:physiology*; D015398:Signal Transduction; D013449:Sulfonamides; D014158:Transcription, Genetic
D002121:Calcium Channel Blockers; D015683:Gene Products, gag; D007546:Isoquinolines; D010703:Phorbol Esters; D010879:Piperazines; D047428:Protein Kinase Inhibitors; D012333:RNA, Messenger; D012191:Retroviridae Proteins; D013449:Sulfonamides; D015124:8-Bromo Cyclic Adenosine Monophosphate; D003994:Bucladesine; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; C053177:N-(2-(methylamino)ethyl)-5-isoquinolinesulfonamide; C043204:N-(2-guanidinoethyl)-5-isoquinolinesulfonamide; D011494:Protein Kinases; D015688:Oncogene Protein pp60(v-src); D011493:Protein Kinase C