Ostrich fructose 1,6-bisphosphatase: distribution of activity and purification of the liver isoenzyme.
1. Fructose 1,6-bisphosphatase was assayed in crude extracts of physiologically important organs and tissues in the ostrich. 2. Highest activity was found in liver and lowest in brain tissue. 3. No activity was detected in the heart, gizzard or adrenals. 4. The enzyme was purified in homogeneous, apparently undegraded form from liver utilizing Blue dextran-Sepharose affinity chromatography. 5. The enzyme is similar to mammalian fructose 1,6-bisphosphatase in many respects including its indispensability of Mg2+ for catalytic activity. 6. Relative molecular weight of the native enzyme and its subunit is about 150,000 and 35,000 respectively. 7. The amino acid composition of ostrich liver fructose 1,6-bisphosphatase is distinctly different from that of the chicken muscle enzyme, but compares favourably with the composition of the rabbit liver enzyme. 8. The purified enzyme is devoid of tryptophan.
van Tonder
A
A
Department of Biochemistry, University of Port Elizabeth, Republic of South Africa.
Naude
R J
RJ
Oelofsen
W
W
D000311:Adrenal Glands / Q000201:enzymology; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001717:Birds / Q000378:metabolism*; D001921:Brain / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D006597:Fructose-Bisphosphatase / Q000302:isolation & purification / Q000378:metabolism*; D005895:Gizzard, Avian / Q000201:enzymology; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D009206:Myocardium / Q000201:enzymology; D009928:Organ Specificity; D013379:Substrate Specificity
D000596:Amino Acids; D007527:Isoenzymes; D006597:Fructose-Bisphosphatase