Moderate Fuchs' endothelial dystrophy ATPase pump site density.
The Na+, K+-ATPase pump site density on corneal endothelial cells from Fuchs' endothelial dystrophy corneas has been shown to be drastically decreased in end-stage disease (McCartney et al, Invest Ophthalmol Vis Sci 28:1955, 1987) and significantly increased in the early stages (Geroski et al, Ophthalmology 92:759, 1985) as compared to normal endothelium. In order to provide values for corneas between these two extremes, eye bank corneas from donors with no evidence of corneal edema but with guttata across the extent of the cornea were processed for autoradiography as well as immunohistochemistry. Pump site density was increased compared to end-stage disease but was less than values reported for either functional tissue or early stage disease. Similarly, immunohistochemistry results showed the amount of Na+, K+-ATPase antibody localization to be increased in respect to end-stage disease, but reduced as compared to functional tissue. These results suggest that pump site density on endothelial cells affected with Fuchs' endothelial dystrophy follows a gradual decline towards end-stage disease values as opposed to a sudden sharp deterioration after an initial increase.
McCartney
M D
MD
Department of Ophthalmology and Visual Sciences, University of Louisville School of Medicine, Kentucky Lions Eye Research Institute, Louisville 40202.
Wood
T O
TO
McLaughlin
B J
BJ
D000368:Aged; D001345:Autoradiography; D003317:Corneal Dystrophies, Hereditary / Q000473:pathology*; D004848:Epithelium; D005260:Female; D005404:Fixatives; D005642:Fuchs' Endothelial Dystrophy / Q000276:immunology / Q000473:pathology*; D006801:Humans; D007158:Immunologic Techniques; D010042:Ouabain / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000276:immunology / Q000378:metabolism*
D005404:Fixatives; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase