title: Human IgE and IgG antibodies to mosquito proteins detected by the immunoblot technique.
abstract: Immunoblot technique was used in this study to detect IgE and IgG antibodies in human sera against mosquito antigens. Mosquito proteins were separated on SDS-polyacrylamide gels and transferred electrophoretically to nitrocellulose papers. After incubation with sera from different individuals, the precipitated bands were analyzed with enzyme-labeled goat anti-human immunoglobulins. Distinct patterns of antigen were recognized by IgE and IgG antibodies.
authors.affiliation: Department of Medical Research, Veterans General Hospital, Taipei, Republic of China.
authors.forename: H D
authors.initials: HD
authors.lastname: Shen
authors.forename: C C
authors.initials: CC
authors.lastname: Chen
authors.forename: H N
authors.initials: HN
authors.lastname: Chang
authors.forename: L Y
authors.initials: LY
authors.lastname: Chang
authors.forename: W C
authors.initials: WC
authors.lastname: Tu
authors.forename: S H
authors.initials: SH
authors.lastname: Han
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D000941:Antigens / Q000276:immunology; D009033:Culicidae / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis*; D008297:Male; D011506:Proteins / Q000276:immunology*
chemical_list: D000941:Antigens; D007074:Immunoglobulin G; D011506:Proteins; D007073:Immunoglobulin E