title: A 10- rather than 9-bp duplication associated with insertion of Tn5 in Escherichia coli K-12.
abstract: The composite transposable element Tn5, which is made up of two inverted IS50 elements surrounding genes encoding drug resistance, generally generates 9-bp duplications at the site of insertion. In our studies of three Tn5 insertion mutants at one location in the Escherichia coli chromosome, we have observed that one contains a duplication of 10 bp, while the other two have the usual 9-bp duplication. Three other insertion elements, IS1, IS4, and IS186, give variable-sized target site sequence duplications. We observed a similarity of amino acid sequence in a small region of the putative transposases among IS4, IS186, and Tn5 suggesting a conservation of function in this group of transposases.
authors.affiliation: Department of Molecular Biology, University of California, Berkeley 94720.
authors.forename: C C
authors.initials: CC
authors.lastname: Chu
authors.forename: A J
authors.initials: AJ
authors.lastname: Clark
mesh_terms: D000595:Amino Acid Sequence; D001482:Base Composition; D001483:Base Sequence; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D009713:Nucleotidyltransferases / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D019895:Transposases
chemical_list: D004251:DNA Transposable Elements; D009713:Nucleotidyltransferases; D019895:Transposases