Rapid screening test for the diagnosis of rotavirus infection.
The early diagnosis of human rotavirus infection is essential for effective patient management and infection control. We report here a rapid, easy-to-perform, and inexpensive test for rotavirus detection. The viral RNA is extracted directly from the stools and electrophoresed on 1% agarose gels. Currently available immunoassays for routine diagnostic purposes are directed at the common group A-specific antigen. As reports become available on human gastroenteritis caused by the atypical or novel rotaviruses, this technique presents an added advantage in that it can detect both group A and non-group A rotaviruses.
Chudzio
T
T
Ottawa Public Health Laboratory, Ontario, Canada.
Kasatiya
S
S
Irvine
N
N
Sankar-Mistry
P
P
D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005069:Evaluation Studies as Topic; D005243:Feces / Q000382:microbiology; D006801:Humans; D012367:RNA, Viral / Q000302:isolation & purification; D012401:Rotavirus / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000175:diagnosis* / Q000382:microbiology; D013997:Time Factors
D012367:RNA, Viral