Leukotriene B4 enhances activation, proliferation, and differentiation of human B lymphocytes.
Highly purified human tonsillar B lymphocytes at different stages of activation were incubated with leukotriene B4 (LTB4). As a key marker for activation, we used the CD23 Ag. LTB4 enhanced the CD23 expression on resting B cells in synergy with B cell-stimulating factors from 4% to 50%. Maximal effect of LTB4 was observed at 10(-10) M to 10(-12) M. LTB4 also augmented the S and M phase entries as well as Ig secretion in synergy with IL-2 and IL-4. In contrast, 5S,12S-dihydroxyeicosatetraenoic acid, an isomer of LTB4, and leukotriene C4 lacked these effects. The results indicate that LTB4 amplifies lymphokine-driven activation, replication, and differentiation of human B lymphocytes.
Yamaoka
K A
KA
Department of Medical Cell Genetics, Karolinska Institute, Stockholm, Sweden.
Claésson
H E
HE
Rosén
A
A
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002675:Child, Preschool; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D007399:Interphase / Q000187:drug effects; D007958:Leukocyte Count / Q000187:drug effects; D007975:Leukotriene B4 / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D011961:Receptors, Fc / Q000378:metabolism; D017455:Receptors, IgE
D000276:Adjuvants, Immunologic; D000944:Antigens, Differentiation, B-Lymphocyte; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011961:Receptors, Fc; D017455:Receptors, IgE; D007975:Leukotriene B4; D004247:DNA