Neutralizing monoclonal antibodies against three serotypes of porcine rotavirus.
Using three serotypes (four strains) of cultivable porcine rotavirus as immunizing antigens, 10 neutralizing monoclonal antibodies were characterized. One VP4-specific monoclonal antibody directed against porcine rotavirus BEN-144 (serotype G4) neutralized human rotavirus strain ST-3 in addition to the homologous porcine virus. All nine VP7-specific monoclonal antibodies were highly specific for viruses of the same serotype as the immunizing rotavirus strain. One exception was the VP7-specific monoclonal antibody C3/1, which neutralized both serotype G3 and G5 rotaviruses. However, this monoclonal antibody did not neutralize the porcine rotavirus AT/76, also of serotype G3, nor mutants of SA-11 virus (serotype G3) which were selected with monoclonal antibody A10/N3 and are known to have mutations affecting the C antigenic region.
Nagesha
H S
HS
School of Microbiology, University of Melbourne, Parkville, Victoria, Australia.
Brown
L E
LE
Holmes
I H
IH
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D000918:Antibody Specificity; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D009500:Neutralization Tests; D012401:Rotavirus / Q000145:classification / Q000235:genetics / Q000276:immunology*; D012703:Serotyping
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral