The selective antigen-presenting cell capacity of activated B lymphocytes in HLA-II-restricted responses of CD4+ T lymphocytes.
We examined the role of antigen-presenting B lymphocytes using panels of antigen-specific CD4+8-T-lymphocyte clones (TLC). All 19 TLC showed a class II major histocompatibility complex-encoded (HLA-II) restricted proliferation to antigen presented by antigen-presenting cells (APC) from the monocyte fraction of peripheral blood. Only six TLC were effectively activated by antigen presented by autologous B lymphocytes activated by EBV transformation. This failure of B lymphocytes was not due to: (i) a high degree of cell surface sialic acid; (ii) a low expression of the cell surface proteins HLA-II, ICAM-1 or LFA-3 that restrict antigen presentation; (iii) lack of secretion of the cytokine IL-1 or other soluble factors that may be required as secondary signals; or (iv) induction of incomplete T-cell activation resulting in the production of growth factor interleukin-2 (IL-2) or the expression of receptors for IL-2 only. These data suggest the involvement of another cell surface interaction in antigen presentation acting besides the interactions known so far.
Van Der Pouw Kraan
T T
TT
Laboratory of Cell Biology Histology, University of Amsterdam, The Netherlands.
Stiekema
F E
FE
Teunissen
M B
MB
Bos
J D
JD
Kapsenberg
L
L
D000938:Antigen-Presenting Cells / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D001685:Biological Factors / Q000502:physiology; D015704:CD4 Antigens / Q000032:analysis; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000276:immunology; D002999:Clone Cells; D016207:Cytokines; D006681:HLA-D Antigens / Q000276:immunology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000276:immunology; D012794:Sialic Acids / Q000502:physiology; D013601:T-Lymphocytes / Q000276:immunology*
D001685:Biological Factors; D015704:CD4 Antigens; D015815:Cell Adhesion Molecules; D016207:Cytokines; D006681:HLA-D Antigens; D008562:Membrane Glycoproteins; D012794:Sialic Acids; D018799:Intercellular Adhesion Molecule-1