Axial ligand replacement in horse heart cytochrome c by semisynthesis.
Semisynthesis has been employed to replace the axial methionine in horse heart cytochrome c with histidine. The reduction potential of the His-80 protein (cyt c-His-80) is 41 mV vs NHE (0.1 M phosphate; pH 7.0; 25 degrees C). The absorption spectra of oxidized and reduced cyt c-His-80 are very similar to those of the native protein in the porphyrin region, but the 695 nm band is absent in the oxidized His-80 protein.
Raphael
A L
AL
Arthur Amos Noyes Laboratory, California Institute of Technology, Pasadena 91125.
Gray
H B
HB
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D003574:Cytochrome c Group; D006418:Heme; D006639:Histidine; D006736:Horses; D008715:Methionine; D009206:Myocardium / Q000201:enzymology*; D011487:Protein Conformation; D013056:Spectrophotometry, Ultraviolet
D003574:Cytochrome c Group; D006418:Heme; D006639:Histidine; D008715:Methionine