title: Expression of (+)-3-PPP binding sites in the PC12 pheochromocytoma cell line.
abstract: Phencyclidine binds with high affinity to both PCP and sigma receptors. We investigated whether the clonal cell line PC12 expressed either of these receptors, and found that these cells contain a haloperidol-sensitive (+)-[3H]3-PPP binding site with a KD of 56 nM, but no PCP binding sites. The (+)3-PPP binding sites in PC12 cells displayed a reversed stereoselectivity for the benzomorphan opiates compared to CNS sigma receptors. Neither nerve growth factor nor sodium butyrate treatment affected the expression of either (+)-3-PPP or TCP binding sites in PC12 cells.
authors.affiliation: Developmental Neurobiology Program, University of Pittsburgh Center for Neuroscience, PA 15213.
authors.forename: Z W
authors.initials: ZW
authors.lastname: Yang
authors.forename: G A
authors.initials: GA
authors.lastname: Paleos
authors.forename: J C
authors.initials: JC
authors.lastname: Byrd
mesh_terms: D000310:Adrenal Gland Neoplasms / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D015259:Dopamine Agents / Q000378:metabolism*; D006801:Humans; D010673:Pheochromocytoma / Q000378:metabolism*; D010880:Piperidines / Q000378:metabolism*; D011869:Radioligand Assay; D011955:Receptors, Drug / Q000378:metabolism*
chemical_list: D015259:Dopamine Agents; D010880:Piperidines; D011955:Receptors, Drug; C026539:preclamol