Analysis of yeast retrotransposon Ty insertions at the CAN1 locus.
The target site distribution for 55 independent Ty insertions that inactivate the function of the Saccharomyces cerevisiae CAN1 gene is reported. Under some selection conditions Ty elements inserted preferentially into the promoter and exhibited an orientation bias. In contrast, under other conditions no insertions were detected in the promoter region and transposition appeared to occur randomly throughout the CAN1 coding sequence. These results show that the target site distribution for Ty insertions may be a function of the selection conditions.
Wilke
C M
CM
Department of Biological Sciences, University of Illinois, Chicago 60680.
Heidler
S H
SH
Brown
N
N
Liebman
S W
SW
D001483:Base Sequence; D002172:Canavanine / Q000378:metabolism; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D004251:DNA Transposable Elements; D005800:Genes, Fungal; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D004251:DNA Transposable Elements; D002172:Canavanine