Chitin derivatives as novel substrates for Trypanosoma brucei brucei attachment in vitro.
Procyclic trypomastigotes of Trypanosoma brucei brucei, cultured in Cunningham's medium with 20% heat-inactivated foetal calf serum at 27 degrees C, attached to chitosan and to gels of N-acetyl chitosan and glycol chitosan. Following attachment, epimastigotes, metacyclic-like trypomastigotes and multinucleate parasites appeared in the culture supernatant.
Wallbanks
K R
KR
Department of Biological Sciences, University of Salford, U.K.
Molyneux
D H
DH
Dirie
M F
MF
D000818:Animals; D002686:Chitin / Q000031:analogs & derivatives* / Q000378:metabolism; D048271:Chitosan; D003470:Culture Media; D005782:Gels; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D014346:Trypanosoma brucei brucei / Q000378:metabolism* / Q000648:ultrastructure
D003470:Culture Media; D005782:Gels; D002686:Chitin; D048271:Chitosan