Comparative effects of F1 and P1 fractions obtained from a Klebsiella pneumoniae glycoproteic extract (RU 41740) on polymorphonuclear leukocytes.
RU 41740 is a glycoprotein extract from Klebsiella pneumoniae described as a macromolecular aggregation of a lipopolysaccharide (LPS)-associated protein (F1 fraction) and a glycoproteic complex (P1 fraction). The human polymorphonuclear (PMN) response was studied after incubation of the cells in the presence of RU 41740, F1 and P1 fractions, or F1-P1 complex. Oxidative metabolism was assessed by chemiluminescence, O2 consumption, O2- generation, and degranulation by beta-glucuronidase release. Results were compared to data obtained with a homologous LPS. RU 41740, F1 fraction, and F1-P1 complex increased the respiratory burst of PMNs stimulated by opsonized zymosan (OZ). N-formylmethionylleucylphenylalanine (fMLP), phorbol myristate acetate, or the calcium ionophore A23187. The beta-glucuronidase release was stimulated by the same compounds when OZ or fMLP were used as stimuli. These effects were dose-dependent. In contrast, P1 fraction was inactive. Addition of polymyxin B resulted in a profound inhibition of both the F1 fraction and LPS activities but only in a partial inhibition of RU 41740 effects. These results strengthen the hypothesis that different biochemical pathways are involved in the enhancement of stimulated neutrophil functions by RU 41740.
el Abbouyi
A
A
Laboratoire de Pharmacologie, Hôpital Cochin, Paris, France.
Paul
J L
JL
Roch-Arveiller
M
M
Sarfati
G
G
Giroud
J P
JP
Raichvarg
D
D
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000494:pharmacology*; D000001:Calcimycin / Q000494:pharmacology; D005591:Chemical Fractionation; D005966:Glucuronidase / Q000097:blood; D006801:Humans; D007711:Klebsiella pneumoniae / Q000032:analysis*; D008163:Luminescent Measurements; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects*; D010101:Oxygen Consumption / Q000187:drug effects; D013481:Superoxides / Q000097:blood; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
D000276:Adjuvants, Immunologic; D001426:Bacterial Proteins; D013481:Superoxides; D000001:Calcimycin; D009240:N-Formylmethionine Leucyl-Phenylalanine; C037567:glycoprotein, Klebsiella pneumoniae; D015054:Zymosan; D005966:Glucuronidase; D013755:Tetradecanoylphorbol Acetate