Proof of the occurrence of 5,6-O-(1-carboxyethylidene)-D-galactofuranose units in the capsular polysaccharide of Klebsiella K12.
The 13C-n.m.r. spectra of the capsular polysaccharide of Klebsiella K41 and phage-derived oligosaccharides K41-P1 and K41-P2 were compared with spectra from the structurally similar polysaccharide of Klebsiella K12 and oligosaccharides K12-P1 and K12-P2. This led to the conclusion that K41 and K12 contain one and two galactofuranose residues per repeating unit, respectively, and that the terminal, lateral residue in K12 has the 5,6-O-(1-carboxyethylidene)-D-galactofuranose structure rather than that of a 4,6-acetal of D-galactopyranose as originally stated. This is the first reported occurrence in Nature of such a structural unit.
Beurret
M
M
Centre de Recherches sur les Macromolécules Végétales, C.N.R.S., associé à l'Université Scientifique et Médicale de Grenoble, Saint-Martin d'Hères, France.
Joseleau
J P
JP
Vignon
M
M
Dutton
G G
GG
Savage
A V
AV
D002240:Carbohydrate Sequence; D005690:Galactose / Q000031:analogs & derivatives* / Q000032:analysis; D007709:Klebsiella / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000032:analysis*; D011522:Protons
D011135:Polysaccharides, Bacterial; D011522:Protons; C061118:5,6-O-(1-carboxyethylidene)galactofuranose; D005690:Galactose