Identification of cell surface receptors for the 86-kilodalton glycoprotein of human cytomegalovirus.
Cell surface receptors for the 86-kDa glycoprotein (gp86) of human cytomegalovirus (HCMV) were identified by using two monoclonal anti-idiotype antibodies that bear the internal image of gp86. These antibodies bound to cells permissive for HCMV infection by both ELISA and immunofluorescence assay and inhibited HCMV plaque formation in human embryonic lung (HEL) cells. Immunoblot analysis showed specific binding of both internal image anti-idiotype antibodies as well as gp86 to an HEL cell membrane protein with an approximate molecular mass of 92.5 kDa. In addition, immunoprecipitation of radiolabeled membrane and cell surface proteins from human foreskin tissue, human foreskin fibroblasts, or HEL cells showed specific binding of anti-idiotype antibody predominantly to the 92.5-kDa protein.
Keay
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Department of Medicine, Stanford University School of Medicine, CA 94305.
Merigan
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TC
Rasmussen
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D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000302:isolation & purification; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D003587:Cytomegalovirus / Q000254:growth & development / Q000276:immunology / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D015151:Immunoblotting; D007130:Immunoglobulin Idiotypes / Q000276:immunology; D008970:Molecular Weight; D011991:Receptors, Virus / Q000302:isolation & purification / Q000378:metabolism*; D010948:Viral Plaque Assay
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D006023:Glycoproteins; D007130:Immunoglobulin Idiotypes; D011991:Receptors, Virus