Isolation and chromosomal localization of the human En-2 gene.
By low stringency hybridization we have isolated from a human cosmid genomic library sequences homologous with a probe from the Drosophila engrailed gene. Partial nucleotide sequence analysis shows a consensus splice acceptor site followed by an open reading frame (ORF) that can encode 104 amino acids; the first 94 amino acids have 71% identity with the Drosophila engrailed protein. The shared region contains a homeo domain and is within the region of engrailed shared with the Drosophila invected gene and the mouse En-1 and En-2 genes. At the amino acid level, the human sequence is 85% identical with the mouse En-1 gene and 100% identical with the mouse En-2 gene. Hybridization against a panel of human-hamster somatic cell hybrids maps this human En-2 gene to chromosome 7, and regional mapping by in situ hybridization to human chromosomes localizes it to region 7q36 at the end of the long arm.
Poole
S J
SJ
Departments of Biochemistry, Howard Hughes Medical Institute, University of California, San Francisco 94143.
Law
M L
ML
Kao
F T
FT
Lau
Y F
YF
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002897:Chromosomes, Human, Pair 7; D004331:Drosophila melanogaster / Q000235:genetics; D005801:Genes, Homeobox; D006801:Humans; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid