tctex-1: a candidate gene family for a mouse t complex sterility locus.
The t complex of the mouse has an important role in male germ cell development and function. Multiple mutations in the t complex interact to alter profoundly the transmission ratio of t complex-bearing sperm or to cause complete sterility or semisterility. We have isolated a multigene family, tctex-1, by screening a testicular cell cDNA library with two reciprocally subtracted testicular cDNA probes. The tctex-1 gene family produces an abundant, virtually germ cell-specific transcript that is 8-fold overexpressed in t homozygotes. The aberrant expression of tctex-1 is solely dependent on the t haplotype genes and occurs only in germ cells. The chromosomal location and pattern of expression of tctex-1 make it a candidate for involvement in male sterility.
Lader
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Department of Zoology, University of Texas, Austin 78712.
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O'Neill
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Artzt
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Bennett
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation; D005796:Genes; D007248:Infertility, Male; D008297:Male; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D010053:Ovary / Q000502:physiology; D012150:Polymorphism, Restriction Fragment Length; D013737:Testis / Q000502:physiology
D004247:DNA