Spin label and microcalorimetric studies of the interaction of DNA with unilamellar phosphatidylcholine liposomes.
High-molecular DNA from chicken erythrocytes interacts with 1,2-dipalmitoylphosphatidylcholine in unilamellar liposomes, both in the presence and absence of Mg2+ ions. This interaction results in a phase separation in liposome membranes. The new phase induced by DNA and Mg2+ has a higher gel-liquid crystal phase transition temperature as measured by microcalorimetry. In the liquid crystalline state, the 16- and 5-doxyl stearic acid spin labels indicate changed local bilayer properties at the label position in the new phase.
Vojcíková
L
L
Faculty of Sciences, P. J. Safárik University, Kosice, Czechoslovakia.
Svajdlenka
E
E
Balgavý
P
P
D015060:1,2-Dipalmitoylphosphatidylcholine; D000818:Animals; D002151:Calorimetry; D002645:Chickens; D004247:DNA / Q000097:blood; D004578:Electron Spin Resonance Spectroscopy; D004912:Erythrocytes / Q000032:analysis; D008081:Liposomes; D008274:Magnesium; D013113:Spin Labels
D008081:Liposomes; D013113:Spin Labels; D015060:1,2-Dipalmitoylphosphatidylcholine; D004247:DNA; D008274:Magnesium