title: Cloning and expression of human arylsulfatase A.
abstract: A full length cDNA for human arylsulfatase A was cloned and sequenced. The predicted amino acid sequence comprises 507 residues. A putative signal peptide of 18 residues is followed by the NH2-terminal sequence of placental arylsulfatase A. One of the arylsulfatase A peptides ends 3 residues ahead of the predicted COOH terminus. This indicates that proteolytic processing of arylsulfatase A is confined to the cleavage of the signal peptide. The predicted sequence contains three potential N-glycosylation sites, two of which are likely to be utilized. The sequence shows no homology to any of the known sequences of lysosomal enzymes but a 35% identity to human steroid sulfatase. Transfection of monkey and baby hamster kidney cells resulted in an up to 200-fold increase of the arylsulfatase A activity. The arylsulfatase A was located in lysosome-like structures and transported to dense lysosomes in a mannose 6-phosphate receptor-dependent manner. The arylsulfatase A cDNA hybridizes to 2.0- and 3.9-kilobase species in RNA from human fibroblasts and human liver. RNA species of similar size were detected in metachromatic leukodystrophy fibroblasts of two patients, in which synthesis of arylsulfatase A polypeptides was either detectable or absent.
authors.affiliation: Biochemie II, Universität Göttingen, Federal Republic of Germany.
authors.forename: C
authors.initials: C
authors.lastname: Stein
authors.forename: V
authors.initials: V
authors.lastname: Gieselmann
authors.forename: J
authors.initials: J
authors.lastname: Kreysing
authors.forename: B
authors.initials: B
authors.lastname: Schmidt
authors.forename: R
authors.initials: R
authors.lastname: Pohlmann
authors.forename: A
authors.initials: A
authors.lastname: Waheed
authors.forename: H E
authors.initials: HE
authors.lastname: Meyer
authors.forename: J S
authors.initials: JS
authors.lastname: O'Brien
authors.forename: K
authors.initials: K
authors.lastname: von Figura
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002553:Cerebroside-Sulfatase / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D005260:Female; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D010920:Placenta / Q000201:enzymology; D010957:Plasmids; D011247:Pregnancy; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
chemical_list: D015342:DNA Probes; D004247:DNA; D002553:Cerebroside-Sulfatase