CD15 antibodies increase neutrophil adhesion to endothelium by an LFA-1-dependent mechanism.
Anti-neutrophil antibodies (CD15) bind to a simple sugar (lactose-N-fucopentaose III, LNF III) known to be present on the chains of the adhesion molecules on neutrophils. We have demonstrated that pre-incubation of neutrophils with a CD15 antibody increases neutrophil adherence to endothelium by a neutrophil-dependent mechanism. This augmented adhesion can be inhibited by antibodies directed against the alpha and beta chain of the leukocyte function-associated antigen 1 (LFA-1) molecule. There is also some increase in surface LFA-1 expression on neutrophils after CD15 incubation, suggesting that CD15 antibodies increase neutrophil adhesion by an LFA-1-dependent mechanism. The increase in LFA-1 expression after CD15 incubation occurs in the presence of a protein synthesis inhibitor. As LFA-1 is not stored intracellularly, the increased adherence of the neutrophils, and increased LFA-1 expression on their surface, suggests the possibility that the CD15 antibodies are binding to the LNF III antigen present on the alpha and beta chains of LFA-1, producing their effect by activating the molecule, perhaps by exposing new antigen sites by a stearic effect.
Forsyth
K D
KD
Department of Immunology, Institute of Child Health, University of London.
Simpson
A C
AC
Levinsky
R J
RJ
D000937:Antigen-Antibody Reactions; D000943:Antigens, Differentiation / Q000032:analysis / Q000276:immunology* / Q000502:physiology*; D018845:CD11 Antigens; D018821:CD18 Antigens; D002448:Cell Adhesion; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D066298:In Vitro Techniques; D016256:Lewis X Antigen; D016169:Lymphocyte Function-Associated Antigen-1; D008562:Membrane Glycoproteins / Q000032:analysis; D009504:Neutrophils / Q000166:cytology*
D000943:Antigens, Differentiation; D018845:CD11 Antigens; D018821:CD18 Antigens; D016256:Lewis X Antigen; D016169:Lymphocyte Function-Associated Antigen-1; D008562:Membrane Glycoproteins