The polypeptide of Mr 14,000 of porcine transmissible gastroenteritis virus: gene assignment and intracellular location.
Synthetic oligopeptides, corresponding to an amino acid sequence encoded by a potential Mr 9000 product's open reading frame (ORF-4) at the 3' terminus of the transmissible gastroenteritis virus genome, were used to generate rabbit antiserum. These antibodies produced immune complexes with an Mr 14,000 (14K) polypeptide in infected cells. The 14K product was shown by immune fluorescence to become associated with the cell nucleus, correlating with the onset of nuclear vacuolation, and suggesting a role in pathogenesis for the ORF-4 gene.
Garwes
D J
DJ
AFRC Institute for Animal Health, Compton Laboratory, Berkshire, U.K.
Stewart
F
F
Britton
P
P
D000595:Amino Acid Sequence; D000818:Animals; D000936:Antigen-Antibody Complex / Q000032:analysis; D002460:Cell Line; D003332:Coronaviridae / Q000235:genetics*; D005796:Genes; D005814:Genes, Viral; D007106:Immune Sera; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010455:Peptides / Q000138:chemical synthesis; D005760:Transmissible gastroenteritis virus / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics* / Q000276:immunology
D000936:Antigen-Antibody Complex; D007106:Immune Sera; D010455:Peptides; D014764:Viral Proteins