Differential distribution of GABAA receptor mRNAs in bovine cerebellum--localization of alpha 2 mRNA in Bergmann glia layer.
Using in situ hybridization histochemistry, we have demonstrated that 3 alpha subunit mRNAs of the GABAA receptor are present in different cell populations of the bovine cerebellum. While the alpha 1 mRNA is the most abundant and is present in granule cells, Purkinje cells and stellate/basket cells, the alpha 2 mRNA appears to be confined to the Bergmann glial cell layer. The alpha 3 mRNA is only expressed in the Golgi cells. This differential distribution of GABAA receptor mRNA subtypes suggests a previously unrecognized complexity of GABAergic transmission in the cerebellum.
Wisden
W
W
MRC Molecular Neurobiology Unit, MRC Centre, Cambridge U.K.
McNaughton
L A
LA
Darlison
M G
MG
Hunt
S P
SP
Barnard
E A
EA
D000818:Animals; D002417:Cattle; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism*; D011963:Receptors, GABA-A / Q000378:metabolism*
D012333:RNA, Messenger; D011963:Receptors, GABA-A