title: Separation of human neutrophils in self-generated continuous density gradients of Percoll.
abstract: Human neutrophils were separated into two fractions using a continuous density gradient of Percoll solution. A marked decrease in O2- production was observed in the low-density neutrophil fraction. There was little erythrocyte contamination in the high-density neutrophil fraction (less than 0.5%), and thus the hypotonic or ammonium chloride lysis of erythrocytes was not necessary.
authors.affiliation: Department of Physiology, Nippon Medical School, Tokyo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Uyesaka
authors.forename: A
authors.initials: A
authors.lastname: Tajiri
authors.forename: K
authors.initials: K
authors.lastname: Arai
mesh_terms: D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D006801:Humans; D009504:Neutrophils / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D011205:Povidone; D012822:Silicon Dioxide
chemical_list: C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone; D010100:Oxygen