Alterations in c-abl gene methylation in cells transformed by phagocyte-generated oxidants.
DNA from 10T1/2 cells transformed by activated neutrophils was analyzed for restriction length polymorphisms (RFLPs) in cellular homologues of retroviral oncogenes, and consistent RFLPs were found in MspI sites of the c-abl gene of all PMN-transformed cell lines. MspI digests probed with c-myc, v-Ki-ras, v-Ha-ras or v-mos showed no RFLPs, and none were observed in EcoRI, PstI, HindIII, BamHI, SmaI, Sau3a, MboI, HhaI, or TaqI digests probed with v-abl. Analysis of HpaII digests supports the conclusion that c-abl RFLPs result from differential methylation of the CCGG HpaII/MspI recognition sequence. MspI RFLPs in the c-abl gene may provide markers for oxidant-related genetic injury.
Weitzman
S A
SA
Department of Medicine, Northwestern University School of Medicine, Chicago, Illinois 60611.
Lee
R M
RM
Ouellette
A J
AJ
D000818:Animals; D015139:Blotting, Southern; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D008745:Methylation; D051379:Mice; D008809:Mice, Inbred C3H; D009504:Neutrophils / Q000502:physiology*; D010586:Phagocytes / Q000502:physiology; D012150:Polymorphism, Restriction Fragment Length; D011519:Proto-Oncogenes
D004247:DNA