title: In vitro infection of cell lines with HTLV-I and SIVmac results in altered intracellular free calcium concentration and increased membrane polarization.
abstract: This study was designed to determine whether retrovirus infection alters basal concentrations of intracellular free calcium, the magnitude of intracellular free calcium change upon receptor-mediated cell-stimulation, and cell membrane polarization. Two retrovirus-infected cell lines were utilized: HTLV-I-infected MT-2 cells and SIV-infected H-9 cells. Uninfected H-9 cells were used as controls. The results show that the retrovirally-infected cells had an increased basal concentration of intracellular free calcium when compared with the control cells and also a reduced magnitude of intracellular free calcium response to receptor-mediated cell stimulants. The retrovirally-infected cell membranes were also hyperpolarized when compared with the control cells.
authors.affiliation: Ohio State University, Department of Veterinary Pathobiology, Columbus 43210.
authors.forename: K A
authors.initials: KA
authors.lastname: Wright
authors.forename: R G
authors.initials: RG
authors.lastname: Olsen
mesh_terms: D002118:Calcium / Q000032:analysis / Q000378:metabolism*; D002460:Cell Line; D002478:Cells, Cultured; D005456:Fluorescent Dyes; D015490:HTLV-I Infections / Q000378:metabolism* / Q000503:physiopathology; D006801:Humans; D008564:Membrane Potentials / Q000502:physiology; D012192:Retroviridae Infections / Q000378:metabolism* / Q000503:physiopathology; D015302:Simian Immunodeficiency Virus
chemical_list: D005456:Fluorescent Dyes; D002118:Calcium