The bimG gene of Aspergillus nidulans, required for completion of anaphase, encodes a homolog of mammalian phosphoprotein phosphatase 1.
In Aspergillus nidulans, the temperature-sensitive, recessive cell cycle mutation bimG11 causes an elevated mitotic index at restrictive temperature and an inability to complete the anaphase separation of daughter nuclei. We have shown that this mutation has an abnormally high content of nuclear phosphoproteins and that the wild-type gene encodes a type 1 protein phosphatase. We conclude that dephosphorylation of a key protein(s) is required to complete mitosis.
Doonan
J H
JH
Department of Pharmacology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School (at Rutgers) Piscataway 08854.
Morris
N R
NR
D000595:Amino Acid Sequence; D000705:Anaphase; D001233:Aspergillus nidulans / Q000166:cytology / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009154:Mutation; D009687:Nuclear Proteins / Q000502:physiology; D010749:Phosphoprotein Phosphatases / Q000235:genetics*; D010750:Phosphoproteins / Q000502:physiology; D054645:Protein Phosphatase 1; D012333:RNA, Messenger / Q000235:genetics; D008941:Spindle Apparatus / Q000502:physiology
D009687:Nuclear Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1