Cytochrome c-551 from the thermophilic bacterium PS3 grown under air-limited conditions.
A small-sized c-type cytochrome, designated cytochrome c-551, was prepared from membrane fraction of the thermophilic bacterium PS3 grown under air-limited conditions by extraction with cholate, precipitation with polyethylene glycol, and successive chromatographies with DEAE-cellulose and Sephacryl S-200 in the presence of a detergent. The purified sample contained approximately 1 mol of heme c per 10,000 g protein; it showed absorption bands at 551, 522 and 416 nm upon reduction, and a Soret peak at 409 nm upon oxidation. This cytochrome showed a single band of 10 kDa on polyacrylamide gel electrophoresis with sodium dodecyl sulfate. The isoelectric point of this cytochrome c-551 was pH 4.0. Cytochrome c-551 was suggested to play an important role in the respiratory chain with a terminal oxidase cytochrome o, which is produced under air-limited conditions, since cytochrome c-551 could mediate electron transfer between cytochrome bc1(b6f) complex and cytochrome o, showing quinol oxidase activity.
Sone
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Department of Biochemistry, Jichi Medical School, Tochigi, Japan.
Kutoh
E
E
Yanagita
Y
Y
D001426:Bacterial Proteins; D003573:Cytochrome b Group; D003574:Cytochrome c Group / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D003580:Cytochromes / Q000378:metabolism; D004579:Electron Transport; D004591:Electrophoresis, Polyacrylamide Gel; D029968:Escherichia coli Proteins; D006094:Gram-Positive Bacteria / Q000032:analysis* / Q000378:metabolism; D006358:Hot Temperature; D007526:Isoelectric Point; D007700:Kinetics; D008970:Molecular Weight; D010084:Oxidation-Reduction; D013057:Spectrum Analysis
D001426:Bacterial Proteins; D003573:Cytochrome b Group; D003574:Cytochrome c Group; D003580:Cytochromes; D029968:Escherichia coli Proteins; C017168:cytochrome bo, E coli; C017167:cytochrome C(551)