Separation of CFU-S from primitive cells responsible for reconstitution of the bone marrow hemopoietic stem cell compartment following irradiation: evidence for a pre-CFU-S cell.
We have studied the in vivo spleen colony-forming ability and marrow repopulating ability of murine bone marrow cells differing in mitochondrial activity. Following centrifugal elutriation the cells were sorted on the basis of rhodamine-123 fluorescence intensity within a predetermined light scatter window. It is shown that a class of hemopoietic stem cells exists that differs from the majority of day-12 spleen colony-forming units (CFU-S) in that it has low mitochondrial activity and a high capacity to generate in time new day-12 CFU-S and cells that rescue recipients from radiation-inflicted death. These data add direct evidence for the identity of pre-CFU-S and CFU-S by physical separation.
Ploemacher
R E
RE
Department of Cell Biology and Genetics, Erasmus University, Rotterdam, The Netherlands.
Brons
R H
RH
D000818:Animals; D001853:Bone Marrow / Q000502:physiology* / Q000528:radiation effects; D016026:Bone Marrow Transplantation; D002469:Cell Separation; D005434:Flow Cytometry; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000145:classification* / Q000528:radiation effects; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D011828:Radiation Chimera; D013154:Spleen / Q000502:physiology* / Q000528:radiation effects / Q000637:transplantation