Monoclonal antibody purification: choice of method and assessment of purity and yield.
In this article we discuss our choices of monoclonal antibody separation methods for the applications which face us most frequently. We explain the rationale behind these choices, to help other users make their own choices. The review is intended to be brief and selective; references to detailed reviews are provided.
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Flinders Medical Centre, Flinders University of South Australia, Bedford Park.
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D017886:Durapatite; D006882:Hydroxyapatites; D007158:Immunologic Techniques; D013205:Staphylococcal Protein A
D000911:Antibodies, Monoclonal; D006882:Hydroxyapatites; D013205:Staphylococcal Protein A; D017886:Durapatite