Immunoregulatory effects of alpha-endorphin, beta-endorphin, methionine-enkephalin, and adrenocorticotropic hormone on anti-tetanus toxoid antibody synthesis by human lymphocytes.
This study assesses the effects of adrenocorticotropic hormone (ACTH), alpha-endorphin (alpha-endo), beta-endorphin (beta-endo), and methionine-enkephalin (met-enk) on tetanus toxoid-stimulated in vitro specific antibody (anti-TT) synthesis. ACTH and TT-costimulated cultures enhanced antibody (Ab) synthesis over control TT-stimulated cultures at 10(-9) and 10(-11) M and suppressed Ab synthesis at 10(-13) and 10(-17) M. The alpha-endo and TT-costimulated cultures enhanced Ab synthesis over control cultures at all concentrations tested (10(-7) to 10(-15) M). Cultures costimulated with beta-endo and TT enhanced Ab synthesis over control cultures at 10(-11) M and suppressed Ab synthesis at doses above and below 10(-11) M. The met-enk and TT-costimulated cultures produced more Ab than controls at 10(-9) M; and the met-enk and TT-costimulated cultures produced less Ab than controls at other concentrations as low as 10(-17) M and as high as 10(-7) M. The results of this study provide one reason as to why the interactions between the neuroendocrine and immune systems remain unclear.
Munn
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Fred Hutchinson Cancer Research Center, Department of Medicine, University of Washington, Seattle 98195.
Lum
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D000324:Adrenocorticotropic Hormone / Q000494:pharmacology*; D000906:Antibodies / Q000276:immunology*; D000917:Antibody Formation / Q000187:drug effects; D002478:Cells, Cultured; D004723:Endorphins / Q000494:pharmacology*; D004744:Enkephalin, Methionine / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D012016:Reference Values; D013745:Tetanus Toxoid / Q000276:immunology*; D018822:alpha-Endorphin; D001615:beta-Endorphin / Q000494:pharmacology*
D000906:Antibodies; D004723:Endorphins; D013745:Tetanus Toxoid; D004744:Enkephalin, Methionine; D001615:beta-Endorphin; D018822:alpha-Endorphin; D000324:Adrenocorticotropic Hormone