[Expression of the Epstein-Barr virus P150 viral capsid antigen in Escherichia coli for the use as antigen in diagnostic tests].
We have attempted to produce the P150 viral capsid antigen of Epstein-Barr Virus (EBV) in Escherichia coli. This protein was found, by immunoprecipitation, to be a clinically relevant antigen, especially for the determination of the IgA-titer in patients with Naso Pharyngeal Carcinoma (NPC). Since the expression of the entire P150 coding region was unsuccessful, we synthesized only the antigenic parts of this protein. After purification of P150 expressed by PUR290CXH580 with column chromatography (sepharose 2B-CL), the resulting product reacts particularly well with IgA antibodies of NPC patients indicating its diagnostic value for NPC.
Fan
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JA
D000956:Antigens, Viral / Q000235:genetics* / Q000302:isolation & purification; D002213:Capsid / Q000276:immunology; D036022:Capsid Proteins; D004274:DNA, Recombinant / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000276:immunology; D015967:Gene Expression Regulation, Viral; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D009303:Nasopharyngeal Neoplasms / Q000175:diagnosis; D010957:Plasmids
D000956:Antigens, Viral; D036022:Capsid Proteins; D004274:DNA, Recombinant; C041788:Epstein-Barr viral capsid antigen; D007070:Immunoglobulin A