Beta 2-adrenoceptor response in the rat uterus at the end of gestation and after induction of labor with RU 486.
To study the relationship between the progesterone environment and beta-adrenoceptors in the myometrium, rats were treated with the antiprogestin RU 486 (10 mg per rat) at 08:30 h on day 21 of gestation. Under these conditions, more than 60% of animals delivered within 24 h after this treatment, while none of the control animals delivered within the same time period. beta-Adrenoceptors were identified using the radiolabeled antagonist (-)-[125I] iodocyanopindolol. The density (Bmax approximately 33-45 fmol/mg protein) and the affinity (KD approximately 0.105-0.106 nM) were not changed (during the late stages of gestation) in RU 486 treated rats compared with control rats. These results were correlated with the relaxation of longitudinal and circular strips of myometrium placed in high KC1 medium and exposed to beta-adrenoceptor agonists. The adrenoceptors implicated in the relaxation of myometrial strips were mainly of the beta 2-subtype. There was no difference in their affinity between control and RU 486 treated rats. Mean pA2 values were 8.46 for propranolol and 8.27 for ICI 118-551 against salbutamol. Altogether these results indicate in the rat that the blockade of the action of progesterone at its receptor site by RU 486 did not modify either the affinity or the sensitivity of beta 2-adrenoceptors in the myometrium, although it induced parturition.
el Alj
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INSERM U262, Physiologie et physiopathologie de la reproduction, Maternité Baudelocque, Paris, France.
Breuiller
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Jolivet
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Ferre
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Germain
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D000420:Albuterol / Q000494:pharmacology; D000818:Animals; D004837:Epinephrine / Q000494:pharmacology; D005260:Female; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D020120:Iodocyanopindolol; D007743:Labor, Obstetric / Q000502:physiology*; D015735:Mifepristone / Q000494:pharmacology*; D009215:Myometrium / Q000187:drug effects / Q000378:metabolism; D010120:Oxytocics; D010869:Pindolol / Q000031:analogs & derivatives / Q000494:pharmacology; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains; D011943:Receptors, Adrenergic, beta / Q000187:drug effects*; D014590:Uterine Contraction / Q000187:drug effects; D014599:Uterus / Q000187:drug effects*
D007457:Iodine Radioisotopes; D010120:Oxytocics; D011943:Receptors, Adrenergic, beta; D015735:Mifepristone; D020120:Iodocyanopindolol; D010869:Pindolol; D000420:Albuterol; D004837:Epinephrine