Identification of a cDNA encoding a long form of prolactin receptor in human hepatoma and breast cancer cells.
Human PRL receptor cDNA clones from hepatoma (Hep G2) and breast cancer (T-47D) libraries were isolated by using a rat PRL receptor cDNA probe. The nucleotide sequence predicts a mature protein of 598 amino acids with a much longer cytoplasmic domain than the rat liver PRL receptor. Although this extended region has additional segments of localized sequence identity with the human GH receptor, there is no identity with any consensus sequences known to be involved in hormonal signal transduction. This cDNA will be a valuable tool to better understand the role of PRL in the development and growth of human breast cancer.
Boutin
J M
JM
Laboratory of Molecular Endocrinology, McGill University, Royal Victoria Hospital, Montreal, Canada.
Edery
M
M
Shirota
M
M
Jolicoeur
C
C
Lesueur
L
L
Ali
S
S
Gould
D
D
Djiane
J
J
Kelly
P A
PA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001943:Breast Neoplasms / Q000235:genetics*; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D003001:Cloning, Molecular; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000302:isolation & purification; D006801:Humans; D066298:In Vitro Techniques; D008113:Liver Neoplasms / Q000235:genetics*; D008969:Molecular Sequence Data; D011817:Rabbits; D051381:Rats; D011981:Receptors, Prolactin / Q000235:genetics*; D011986:Receptors, Somatotropin / Q000648:ultrastructure; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
D015342:DNA Probes; D004273:DNA, Neoplasm; D011981:Receptors, Prolactin; D011986:Receptors, Somatotropin