Effects of hydroxyurea on the phagocytosis of microspheres in V79 cells.
Phagocytosis of fluorescent microspheres in V79 cells arrested in the S phase by hydroxyurea (HU) was investigated by flow cytometry. The phagocytic activity increased with the exposure time of HU. No dependency on the HU concentration was observed in the concentration used. Cell size (projection area) and membrane fluidity, two factors effected by HU treatment, were examined in relation to phagocytic activity, and were found to be significantly increased. The elevation in phagocytic activity could be interpreted in terms of the alteration in the two physiological factors.
Fujikawa-Yamamoto
K
K
Department of Pathology, Kanazawa Medical University, Ishikawa, Japan.
Odashima
S
S
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology / Q000648:ultrastructure; D006224:Cricetinae; D003412:Cricetulus; D004578:Electron Spin Resonance Spectroscopy; D005434:Flow Cytometry; D006918:Hydroxyurea / Q000494:pharmacology*; D008168:Lung / Q000166:cytology* / Q000648:ultrastructure; D008560:Membrane Fluidity / Q000187:drug effects; D008863:Microspheres; D010587:Phagocytosis / Q000187:drug effects*
D006918:Hydroxyurea