Localization of human papillomavirus type 16 DNA using the polymerase chain reaction in the cervix uteri of women with cervical intraepithelial neoplasia.
The localization of human papillomavirus type 16 (HPV-16) DNA throughout the cervix uteri of women with cervical intraepithelial neoplasia (CIN) was studied by utilizing the polymerase chain reaction technique directly on histologically defined sections of paraffin-embedded cervical tissue obtained by conizations. HPV-16 DNA was detected only in the sections that contained CIN lesions and/or koilocytes. No HPV-16 DNA was detected in sections that contained only normal epithelium. This is in accordance with HPV-16 playing a role in the development of CIN lesions.
Cornelissen
M T
MT
Department of Medical Microbiology, University of Amsterdam, The Netherlands.
van den Tweel
J G
JG
Struyk
A P
AP
Jebbink
M F
MF
Briët
M
M
van der Noordaa
J
J
ter Schegget
J T
JT
D002584:Cervix Uteri / Q000382:microbiology*; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D005260:Female; D006801:Humans; D009841:Oligonucleotides; D027383:Papillomaviridae / Q000235:genetics*; D010232:Paraffin; D016133:Polymerase Chain Reaction; D014412:Tumor Virus Infections / Q000175:diagnosis*; D002583:Uterine Cervical Neoplasms / Q000382:microbiology*
D004279:DNA, Viral; D009841:Oligonucleotides; D010232:Paraffin