Purification and some properties of a 2Fe ferredoxin in Pseudomonas ovalis.
A [2Fe-2S] ferredoxin was found in Pseudomonas ovalis which was grown in a medium supplemented with glucose and ammonium sulfate. The molecular weight of the 2Fe ferredoxin was estimated to be 13,000. It contained 2.2 gramatoms of non-heme iron and 2.3 gramatoms of acid-labile sulfur per mole protein. The absorption and circular dichroism spectra were characteristic of those of [2Fe-2S] type ferredoxins, especially adrenodoxin and putidaredoxin. The electron paramagnetic resonance spectrum of the reduced protein showed an axial symmetry (g = 2.020, g = 1.939). The amino acid composition was determined.
Ohmori
D
D
Department of Chemistry, School of Medicine, Juntendo University, Chiba, Japan.
Yamakura
F
F
Suzuki
K
K
D000596:Amino Acids / Q000032:analysis; D002942:Circular Dichroism; D004578:Electron Spin Resonance Spectroscopy; D005288:Ferredoxins / Q000145:classification / Q000302:isolation & purification*; D015394:Molecular Structure; D010084:Oxidation-Reduction; D011549:Pseudomonas / Q000032:analysis*
D000596:Amino Acids; D005288:Ferredoxins