Effect of disodium cromoglycate on deuterium oxide-induced degranulation of human eosinophils in allergic asthmatic patients in vitro.
Eosinophils from many atopic asthmatic patients have been shown to degranulate in response to 44% deuterium oxide, in contrast to eosinophils from healthy persons. Several pieces of evidence have been obtained recently, indicating that disodium cromoglycate (DSCG) may act on eosinophils. In the present study we show, that prior incubation of eosinophils obtained from the blood of 26 of 30 atopic asthmatic deuterium oxide-responders with DSCG significantly inhibited D2O-induced eosinophil degranulation in the dose dependent manner. Eosinophil degranulation was assayed using haemacytomether method. These results suggest, that DSCG may have direct action on eosinophils.
Pałczyński
C
C
Department of Pneumonology and Allergology, Medical Academy, Lódź.
Górski
P
P
Grzegorczyk
J
J
D000328:Adult; D001249:Asthma / Q000097:blood*; D015550:Cell Degranulation / Q000187:drug effects*; D004205:Cromolyn Sodium / Q000494:pharmacology*; D003903:Deuterium / Q000494:pharmacology*; D017666:Deuterium Oxide; D004804:Eosinophils / Q000187:drug effects* / Q000276:immunology; D005260:Female; D006801:Humans; D008297:Male; D014867:Water / Q000494:pharmacology*
D014867:Water; D003903:Deuterium; D017666:Deuterium Oxide; D004205:Cromolyn Sodium