Localization of DNA repair synthesis by human cell extracts to a short region at the site of a lesion.
Double-stranded bacteriophage M13 DNA molecules were constructed containing a single specifically placed 2-(acetylamino)fluorene adduct or a single 4'-hydroxymethyl-4,5',8-trimethylpsoralen monoadduct. These circular DNA molecules were used to analyze in vitro DNA repair synthesis by cell extracts from normal human lymphoid cell lines. Both types of lesions stimulate DNA repair synthesis at the site of the adduct. DNA repair synthesis induced by the 2-(acetyl-amino)fluorene adduct took place in the damaged strand and was confined to a region within a 31-base pair restriction fragment around the adduct.
Hansson
J
J
Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, United Kingdom.
Munn
M
M
Rupp
W D
WD
Kahn
R
R
Wood
R D
RD
D015073:2-Acetylaminofluorene / Q000494:pharmacology; D001483:Base Sequence; D002460:Cell Line; D002472:Cell Transformation, Viral; D002474:Cell-Free System; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004260:DNA Repair; D004261:DNA Replication; D004270:DNA, Circular / Q000187:drug effects / Q000235:genetics*; D004279:DNA, Viral / Q000187:drug effects / Q000235:genetics; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D015183:Restriction Mapping; D014307:Trioxsalen / Q000031:analogs & derivatives / Q000494:pharmacology
D003432:Cross-Linking Reagents; D004270:DNA, Circular; D004279:DNA, Viral; D015345:Oligonucleotide Probes; C016507:hydroxymethyltrioxsalen; D015073:2-Acetylaminofluorene; D014307:Trioxsalen