System A amino acid transport in a rat submandibular ductal cell line.
1. Neutral amino acid transport was studied in an established cell line derived from rat submandibular glands, RSMTx. 2. The greatest portion of alpha-amino isobutyrate (AIB) transport is mediated by system A. This component is Na+ dependent, pH sensitive, markedly inhibited by methyl AIB and enhanced 2-5-fold by amino acid depletion. 3. Evidence for the presence of other neutral amino acid transport systems, presumably ASC and L, was also found in these cells.
Brown
A M
AM
Food and Drug Administration, Center for Devices and Radiological Health, Rockville, MD 20857.
Baum
B J
BJ
D000222:Adaptation, Physiological; D000596:Amino Acids / Q000172:deficiency / Q000378:metabolism*; D000621:Aminoisobutyric Acids / Q000378:metabolism; D000818:Animals; D001693:Biological Transport, Active; D002460:Cell Line; D007700:Kinetics; D008297:Male; D051381:Rats; D012964:Sodium / Q000502:physiology; D013363:Submandibular Gland / Q000166:cytology / Q000378:metabolism*
D000596:Amino Acids; D000621:Aminoisobutyric Acids; C100049:2-aminoisobutyric acid; D012964:Sodium