The establishment of Epstein-Barr virus nuclear antigen-positive (SP-50B) and Epstein-Barr virus nuclear antigen-negative (SP-53) cell lines with t(11;14)(q13;q32) chromosome abnormality from an intermediate lymphocytic lymphoma.
Two lymphoma cell lines, SP-50B and SP-53, were established from peripheral blood of a 58-year-old woman with leukemic conversion of intermediate lymphocytic lymphoma. These cell lines grew in suspension with or without forming clumps of cells. SP-50B was morphologically similar to the common Epstein-Barr (EB) virus-transformed lymphoblastoid cell lines and was positive for EB virus nuclear antigen (EBNA), whereas SP-53 closely resembled the patient's lymphoma cells and was negative for EBNA. Both cell lines expressed the same phenotypic markers as original lymphoma cells (CpIg+, SmIg+, OKIa1+, Leu12+) and possessed t(11;14)(q13;q32) chromosome translocation. These results indicate that although morphologically different, SP-50B and SP-53 were both derived from patient's lymphoma cells. The long-term cultivation of EBNA-positive and EBNA-negative B-cell lymphoma lines from a single donor has not been previously reported. These cell lines would provide useful tools for studying the oncogenic role of EB virus and bcl-1 oncogene that is located on chromosome 11q13.
Daibata
M
M
Department of Medicine, Kochi Medical School, Japan.
Kubonishi
I
I
Eguchi
T
T
Yano
S
S
Ohtsuki
Y
Y
Miyoshi
I
I
D000956:Antigens, Viral / Q000032:analysis*; D000957:Antigens, Viral, Tumor / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis; D002880:Chromosomes, Human, Pair 11; D002883:Chromosomes, Human, Pair 14; D019309:Epstein-Barr Virus Nuclear Antigens; D005260:Female; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D007621:Karyotyping; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics* / Q000276:immunology; D008875:Middle Aged; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
D000956:Antigens, Viral; D000957:Antigens, Viral, Tumor; D014408:Biomarkers, Tumor; D019309:Epstein-Barr Virus Nuclear Antigens