Application of the electrochemistry of cytochrome c to the measurement of superoxide radical production.
An electrochemical sensor for the measurement of the superoxide anion produced as a result of the respiratory burst of neutrophils is described. The reduction of cytochrome c by the superoxide ion was monitored using a surface-modified gold electrode. The current produced was shown to be superoxide-specific and proportional to the number of neutrophils in both purified neutrophil preparations and plasma.
McNeil
C J
CJ
Department of Clinical Biochemistry, Medical School, University of Newcastle upon Tyne, England.
Smith
K A
KA
Bellavite
P
P
Bannister
J V
JV
D003574:Cytochrome c Group / Q000378:metabolism*; D004563:Electrochemistry; D004566:Electrodes; D005609:Free Radicals; D006801:Humans; D066298:In Vitro Techniques; D009504:Neutrophils / Q000378:metabolism; D013053:Spectrophotometry; D013481:Superoxides / Q000378:metabolism*; D019820:Xanthine; D014969:Xanthine Oxidase / Q000378:metabolism; D014970:Xanthines / Q000378:metabolism
D003574:Cytochrome c Group; D005609:Free Radicals; D014970:Xanthines; D013481:Superoxides; D019820:Xanthine; D014969:Xanthine Oxidase