title: Immunohistologic study of pulmonary and lymphatic tissues from gnotobiotic pigs inoculated with ara-T-resistant strain of pseudorabies virus.
abstract: Ara-T-resistant strain of pseudorabies virus (PRV) was inoculated intranasally into six 2-week-old gnotobiotic pigs. Five inoculated pigs were sneezing and coughing. In pigs 1 to 4 killed on postinoculation days (PID) 3, 5, 7, and 9, respectively, PRV antigen was detected in respiratory epithelial cells, and pigs had severe pneumonitis. In pigs 5 and 6 killed on PID 11 and 13, respectively, PRV antigen was localized in macrophages in alveoli and necrotizing nodules. Immunoglobulin-containing cells (IgG, IgM, and IgA) were detected first in pneumonic lesions in pig 4 killed on PID 9. Detection of immunoglobulin-containing cells was coincident with pulmonary inflammation and regeneration of pneumonic lesions. The number of IgG-containing cells was greater than that of IgM- and IgA-containing cells. Corresponding to transient viral multiplication, IgG-, IgM-, and IgA-containing cells were demonstrated first in lymphatic tissues in pig 1 killed on PID 3 and their number was 5 to 10 times more than those in control pigs 7 and 8. Seemingly, PRV replication in lymphatic tissues stimulated the proliferative response of specific immunoglobulin-producing cells, and the appearance of immunoglobulin-containing cells in the lungs was associated with clearance of PRV and regeneration of pneumonic lesions.
authors.affiliation: National Institute of Animal Health, Ibaraki, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Narita
authors.forename: T
authors.initials: T
authors.lastname: Imada
authors.forename: M
authors.initials: M
authors.lastname: Haritani
authors.forename: H
authors.initials: H
authors.lastname: Kawamura
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001980:Bronchi / Q000276:immunology; D005856:Germ-Free Life; D011558:Herpesvirus 1, Suid / Q000276:immunology / Q000302:isolation & purification; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007150:Immunohistochemistry; D008168:Lung / Q000382:microbiology / Q000473:pathology*; D008198:Lymph Nodes / Q000276:immunology / Q000382:microbiology / Q000473:pathology*; D009297:Nasal Mucosa / Q000382:microbiology; D014066:Palatine Tonsil / Q000276:immunology / Q000382:microbiology; D011557:Pseudorabies / Q000276:immunology / Q000473:pathology*; D011650:Pulmonary Alveoli / Q000276:immunology; D013154:Spleen / Q000276:immunology / Q000382:microbiology; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000473:pathology*; D014132:Trachea / Q000382:microbiology
chemical_list: D000956:Antigens, Viral; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M