title: Ovarian follicular fluid eicosanoid concentrations during the pre-ovulatory period in humans.
abstract: Prostaglandins are involved in ovulation and in every mammal studied so far, ovulation has been inhibited by prostaglandin inhibition. Information regarding the role of leukotrienes and thromboxanes in ovulation is more limited. In order to study the production of eicosanoids in human pre-ovulatory follicular fluid, follicular aspiration was timed by means of serial ultrasound scans and human chorionic gonadotrophin (hCG) to be immediately pre-ovulatory. 11 women were studied and the eicosanoids measured by radioimmunoassay (RIA). The follicular fluid was found to contain leukotrienes (LT) B4, LTC4 (D4, E4), prostaglandin (PG) E2, PGF2 alpha 6 keto PGF1 alpha k and thromboxane (TX) B2. This is the first published report of leukotrienes in human follicular fluid in spontaneous cycles, and is one of the few reports showing prostaglandins and thromboxanes. The significance of demonstrating leukotrienes in human follicular fluid is discussed as is the correlation between individual eicosanoids in the human ovary.
authors.affiliation: Department of Obstetrics and Gynaecology, University Hospital of South Manchester, England.
authors.forename: A R
authors.initials: AR
authors.lastname: Priddy
authors.forename: S R
authors.initials: SR
authors.lastname: Killick
authors.forename: M
authors.initials: M
authors.lastname: Elstein
authors.forename: J
authors.initials: J
authors.lastname: Morris
authors.forename: M
authors.initials: M
authors.lastname: Sullivan
authors.forename: L
authors.initials: L
authors.lastname: Patel
authors.forename: M G
authors.initials: MG
authors.lastname: Elder
mesh_terms: D015232:Dinoprostone / Q000032:analysis; D005260:Female; D005498:Follicular Phase; D006801:Humans; D007975:Leukotriene B4 / Q000032:analysis; D015289:Leukotrienes / Q000032:analysis*; D008084:Lipoxygenase / Q000378:metabolism; D006080:Ovarian Follicle / Q000032:analysis* / Q000201:enzymology; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D011460:Prostaglandins F / Q000032:analysis; D013189:SRS-A / Q000032:analysis; D013929:Thromboxane B2 / Q000032:analysis
chemical_list: D015289:Leukotrienes; D011460:Prostaglandins F; D013189:SRS-A; D007975:Leukotriene B4; D013929:Thromboxane B2; D008084:Lipoxygenase; D011451:Prostaglandin-Endoperoxide Synthases; D015232:Dinoprostone