title: A rapid method for detection of flavivirus antigens: staphylococcal co-agglutination test using monoclonal antibodies to Japanese encephalitis virus.
abstract: Staphylococcus aureus rich in protein A when coated with monoclonal antibodies (MoAb) to Japanese encephalitis virus (JEV) gave a highly specific reaction with flavivirus antigens. The bacteria coated with JEV species-specific MoAb gave a strong co-agglutination with fifty-six JEV isolates from various parts of China, but no co-agglutination with Murray Valley encephalitis (MVE) and Kunjin (Kun) virus antigens. The flavivirus- and subgroup-specific MoAbs were reactive with MVE and Kun, as well as with the majority of the JEV strains. Blocking test with homologous MoAbs abolished co-agglutination further confirming its specificity. Numerous virus particles were observed on the surface of MoAb-coated staphylococci under the electron microscope after co-agglutination. The test appeared rapid, specific, simple to perform, and useful for rapid detection and identification of flaviviruses.
authors.affiliation: Institute of Virology, Chinese Academy of Preventive Medicine, Beijing.
authors.forename: Y H
authors.initials: YH
authors.lastname: Zhang
authors.forename: W F
authors.initials: WF
authors.lastname: Yu
authors.forename: J
authors.initials: J
authors.lastname: Cai
authors.forename: D
authors.initials: D
authors.lastname: Qian
authors.forename: T X
authors.initials: TX
authors.lastname: Zhao
authors.forename: Z Z
authors.initials: ZZ
authors.lastname: Xu
authors.forename: M X
authors.initials: MX
authors.lastname: Wan
mesh_terms: D000372:Agglutination Tests; D000911:Antibodies, Monoclonal / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology; D002462:Cell Membrane / Q000276:immunology; D003429:Cross Reactions; D004664:Encephalitis Virus, Japanese / Q000276:immunology; D005416:Flavivirus / Q000276:immunology / Q000302:isolation & purification*; D006385:Hemagglutination Inhibition Tests; D006863:Hydrogen-Ion Concentration; D009500:Neutralization Tests; D012680:Sensitivity and Specificity; D013211:Staphylococcus aureus / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000956:Antigens, Viral