title: [An alpha-satellite DNA sequence, alpha-RI-6, specific for human chromosomes 13 and 21, detected using the RFLP technic with digoxigenin labelled probes].
abstract: The authors compared two at present most widely used techniques for labelling DNA probes: a) radioactive labelling by means of the radioisotope 32P; non-radioactive labelling using the hapten digoxigenin for the visualization of the hybridization process on nylon membranes. Then sensitivity of the technique of non-radioactive labelling of heterochromatin probes was equivalent to the radioactive method.
authors.forename: I
authors.initials: I
authors.lastname: Mazura
authors.forename: R
authors.initials: R
authors.lastname: Brdicka
authors.forename: R J
authors.initials: RJ
authors.lastname: Srám
authors.forename: J
authors.initials: J
authors.lastname: Jurov
authors.forename: J
authors.initials: J
authors.lastname: Kapras
mesh_terms: D015139:Blotting, Southern; D002882:Chromosomes, Human, Pair 13; D002891:Chromosomes, Human, Pair 21; D015342:DNA Probes; D004276:DNA, Satellite / Q000032:analysis / Q000235:genetics*; D004076:Digoxigenin; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D015342:DNA Probes; D004276:DNA, Satellite; D004076:Digoxigenin
vernacular_title: Alfa-satelitní DNA-sekvence, alfa-RI-6, specificka pro lidské chromosomy 13 a 21, detekovaná pomocí RFLP-techniky s digoxigeninovým znacením sondy.