Metabolism of LTC4 to LTD4 and LTE4 in isolated guinea pig lung lobes.
Leukotrienes are known to be easily metabolized to other substances. But the metabolic fates of LTC4 and LTD4 have not been established in the intact lung. In this investigation we perfused isolated guinea pig lung lobes and injected synthesized LTC4 and LTD4. The effluent was assayed by HPLC. LTD4 and LTE4 were detected following perfusion of LTC4, and LTE4 was detected following perfusion of LTD4. These results suggest that perfused guinea pig lung lobes may metabolize LTC4 to LTD4 and LTE4, and LTD4 to LTE4.
Kobayashi
J
J
Department of Pulmonary Medicine, Jichi Medical School, Tochigi, Japan.
Kitamura
S
S
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004492:Edetic Acid / Q000494:pharmacology; D006168:Guinea Pigs; D066298:In Vitro Techniques; D017999:Leukotriene E4; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D010477:Perfusion; D013189:SRS-A / Q000031:analogs & derivatives* / Q000096:biosynthesis* / Q000378:metabolism*
D013189:SRS-A; D017999:Leukotriene E4; D004492:Edetic Acid