Alkaline secretion by Necturus proximal duodenal mucosa.
Proximal duodenum from the amphibian Necturus was stripped of muscle layers and the mucosa was mounted as a tube for studies of alkali transport or as a flat sheet for intracellular impalement by voltage-sensitive glass micro-electrodes. The mucosa alkalinized the unbuffered luminal perfusate at a high rate (3.4 muequiv. cm-1 h-1) and developed a transepithelial electric potential difference of 5.7 mV (lumen negative). Transport was inhibited by 2,4-dinitrophenol (10(-4) M) and by furosemide (10(-3) M) and SITS (10(-3) M) on the seros but not on the mucosal side, indicating dependence on tissue metabolism and on serosal membrane Cl-/HCO3- exchange. Prostaglandin E2 (10(-7)-10(-5) M) and dibutyryl cyclic AMP (10(-6)-10(-4) M) had no effects on the secretion or transepithelial electrical potential difference. removal of serosal HCO3- decreased luminal alkalinization by 75%, indicating a contribution by passive migration of HCO3- and/or a dependence of transcellular transport on the nutrient supply of this ion. Administration of HCO3- (17.8 mM) to the luminal perfusate affected neither the transepithelial nor transmembrane electrical potential differences nor the resistance ratio. It is thus unlikely that the luminal membrane possesses any major HCO3- conductance.
Bridén
S
S
Department of Physiology and Medical Biophysics, Uppsala University Biomedical Centre, Sweden.
Flemström
G
G
D019297:2,4-Dinitrophenol; D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D001639:Bicarbonates / Q000378:metabolism*; D003994:Bucladesine / Q000494:pharmacology; D004140:Dinitrophenols / Q000494:pharmacology; D015232:Dinoprostone / Q000494:pharmacology; D004386:Duodenum / Q000187:drug effects / Q000502:physiology*; D005665:Furosemide / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism*; D009338:Necturus maculosus; D014475:Uncoupling Agents
D001639:Bicarbonates; D004140:Dinitrophenols; D014475:Uncoupling Agents; D003994:Bucladesine; D005665:Furosemide; D015232:Dinoprostone; D019297:2,4-Dinitrophenol; D007213:Indomethacin