Role of the membrane-associated folate binding protein (folate receptor) in methotrexate transport by human KB cells.
The uptake of methotrexate by KB cells was observed to be dependent on time, temperature, and concentration of extracellular methotrexate. The Kd for methotrexate surface binding to KB cells was approximately 200 nM. Following exposure of KB cells to trace quantities of [3H]methotrexate for periods ranging from 6 min to 24 h, the cellular methotrexate was progressively formed into methotrexate polyglutamates and was bound to dihydrofolate reductase as well as to a particulate folate binding protein. To further study the mechanism of methotrexate uptake in KB cells, the N-hydroxysuccinimide ester of methotrexate was used to covalently label the surface of KB cells and to inhibit transport of methotrexate. The N-hydroxysuccinimide ester of methotrexate was bound to a species of protein with an apparent molecular weight of 160,000 in 1% (v/v) Triton X-100 that bound folic acid and was specifically precipitated by antiserum raised against the previously purified high-affinity folate binding protein (the folate receptor) from human KB cells. In addition, trypsin was utilized to remove surface-accessible covalently bound methotrexate. The amount of covalently bound methotrexate that could be released by trypsin initially decreased on incubation at 37 degrees C, suggesting that the methotrexate and binding protein were internalized. However, with time, trypsin could again release the covalently bound methotrexate, suggesting that the binding protein cycles from the external cell surface to the inside of the cell and out again.
Deutsch
J C
JC
Department of Medicine, University of Colorado Health Sciences Center, Denver 80262.
Elwood
P C
PC
Portillo
R M
RM
Macey
M G
MG
Kolhouse
J F
JF
D001667:Binding, Competitive; D001692:Biological Transport; D002352:Carrier Proteins / Q000502:physiology*; D002850:Chromatography, Gel; D058973:Folate Receptors, GPI-Anchored; D005492:Folic Acid / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D007624:KB Cells / Q000378:metabolism*; D007700:Kinetics; D008727:Methotrexate / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000502:physiology*; D013388:Succinimides; D013696:Temperature; D014357:Trypsin
D002352:Carrier Proteins; D058973:Folate Receptors, GPI-Anchored; D011956:Receptors, Cell Surface; D013388:Succinimides; D005492:Folic Acid; D014357:Trypsin; C001426:N-hydroxysuccinimide; D008727:Methotrexate