title: Myeloproliferative disorders: usefulness of X-linked probes in diagnosis.
abstract: Myeloproliferative disorders are neoplasms of the pluripotent hematopoietic stem cell. Accurate diagnosis and distinction from reactive processes can be difficult therein with cytogenetic analysis only being useful in a minority of patients. Use of X-linked restriction fragment length polymorphism and methylation analysis has enabled clonal analysis to be performed in up to 50% of females, significantly increasing the proportion of analyzable patients over methods dependent on glucose-6-phosphate dehydrogenase heterozygosity. Using hypoxanthine phosphoribosyl transferase and phosphoglycerate kinase probes, we have demonstrated monoclonality of peripheral blood leukocytes in three females with myeloproliferative disorders who had uninformative chromosomal analysis. This technique greatly enhances the diagnosis of early myeloproliferative disorder.
authors.affiliation: Department of Hematology, University of Texas M. D. Anderson Cancer Center, Houston 77030.
authors.forename: K M
authors.initials: KM
authors.lastname: Taylor
authors.forename: M
authors.initials: M
authors.lastname: Shetta
authors.forename: M
authors.initials: M
authors.lastname: Talpaz
authors.forename: H M
authors.initials: HM
authors.lastname: Kantarjian
authors.forename: S
authors.initials: S
authors.lastname: Hardikar
authors.forename: A C
authors.initials: AC
authors.lastname: Chinault
authors.forename: K B
authors.initials: KB
authors.lastname: McCredie
authors.forename: G
authors.initials: G
authors.lastname: Spitzer
mesh_terms: D000328:Adult; D015139:Blotting, Southern; D004247:DNA / Q000032:analysis / Q000378:metabolism; D015342:DNA Probes; D005260:Female; D008040:Genetic Linkage; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D007962:Leukocytes / Q000032:analysis; D008745:Methylation; D008875:Middle Aged; D009196:Myeloproliferative Disorders / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010735:Phosphoglycerate Kinase / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D011237:Predictive Value of Tests; D014960:X Chromosome
chemical_list: D015342:DNA Probes; D004247:DNA; D007041:Hypoxanthine Phosphoribosyltransferase; D010735:Phosphoglycerate Kinase