Identification and characterization of the BPV-2 L2 protein.
The bovine papilloma virus type 2 (BPV-2) L2 open reading frame was cloned into a lambda pL promoter expression vector. This plasmid was shown to express a fusion protein which constituted 75% of the BPV-2 L2 ORF linked to the first 13 N-terminal amino acids of the lambda cll gene product. Antisera generated against this fusion protein were used to identify the L2 gene product as a 64,000-Da protein in BPV-2 virions. Western blot analysis demonstrated that the L2 viral protein was present in full capsids and in small amounts in empty capsids. Densitometer analysis indicated that the L2 protein constituted only 8% of the total L1 + L2 protein content of full capsids. Antisera was also used to demonstrate that the BPV-2 L2 protein is antigenically related to the BPV-1 L2 protein.
Rippe
R A
RA
Department of Microbiology and Immunology, University of Arizona, Tucson 85724.
Meinke
W J
WJ
D000911:Antibodies, Monoclonal / Q000276:immunology; D052688:Bovine papillomavirus 1 / Q000032:analysis / Q000235:genetics*; D002213:Capsid / Q000032:analysis; D003001:Cloning, Molecular; D005814:Genes, Viral; D008970:Molecular Weight; D027383:Papillomaviridae / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000276:immunology; D012995:Solubility; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics*
D000911:Antibodies, Monoclonal; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins