Cytoskeletal protein and GTP-ase profiles differ depending on the degree of transformation of fibroblasts.
Four fibroblast cell lines of differing rates of cell division (1 normal and 3 transformed to differing degrees) were cultured in 10% FCS and 90% DMEM. The cultures were maintained for 72 hours, either dosed with C18 fatty acids or retained as controls, and cell viability was then assessed using the Trypan Blue exclusion method. The cytoskeletal proteins were then prepared, and the protein and GTP-ase profiles determined. No changes in profile were detected within a cell type whether dosed with fatty acid or not. Between cell types, whilst total cytoskeletal protein remained constant, distinct changes were observed in the PAGE. The more transformed the cells, the fewer protein bands detected, especially those with masses of 50-60kD. Tubulin has sub-units with masses in this range, and also expresses GTP-ase activity. This activity decreased in parallel to the changes in protein profiles.
Girao
L A
LA
Department of Medical Biochemistry, University of the Witwatersrand Medical School, Johannesburg, South Africa.
Giangregorio
A
A
Davidson
B C
BC
D002455:Cell Division; D002461:Cell Line, Transformed; D003598:Cytoskeletal Proteins / Q000032:analysis*; D005347:Fibroblasts / Q000032:analysis / Q000473:pathology; D020558:GTP Phosphohydrolases / Q000032:analysis*; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis*
D003598:Cytoskeletal Proteins; D010744:Phosphoric Monoester Hydrolases; D020558:GTP Phosphohydrolases