[The role of single-stranded DNA in the integration of SV40 genome into cellular DNA].
The integration of temperature-sensitive SV40 mutant DNA (tsA239) into the Chinese hamster cellular genome at an early stage of infection was studied. The content of single-stranded DNA structures in the infected and control cells at a non-permissive temperature (40 degrees C) differed drastically from that in control cells at permissive temperatures (33 degrees C, 37 degrees C). The role of single-stranded structures in the integration of the SV40 genome into cellular DNA was shown by blot hybridization. The integration mechanism is discussed.
Popov
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D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D009154:Mutation; D009693:Nucleic Acid Hybridization; D011995:Recombination, Genetic; D013539:Simian virus 40 / Q000235:genetics* / Q000502:physiology; D014779:Virus Replication
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004247:DNA
Issledovanie uchastiia odnonitevoĭ DNK v integratsii genoma SV40 c DNK kletok.