Cyclic GMP and nitroprusside inhibit the activation of human platelets by fluoroaluminate.
Sodium nitroprusside, an activator of the soluble guanylate cyclase, inhibits the intracellular Ca2+ mobilization, ATP secretion and aggregation of human platelets evoked by fluoroaluminate. Similar results are obtained with 8-bromo-cyclic GMP (8-Br-cGMP). Both nitroprusside and 8-Br-cGMP inhibit the protein kinase C-dependent phosphorylation of the 47 and 20 kDa proteins induced by fluoroaluminate, but not by the protein kinase C activators phorbol ester and diacylglycerol. Since fluoroaluminate interacts directly with a G protein, the present results suggest that the cGMP interferes with platelet activation at the level of G protein-phospholipase C.
Deana
R
R
Department of Biological Chemistry, C.N.R. Unit for the Study of Mitochondrial Physiology, University of Padova, Italy.
Ruzzene
M
M
Doni
M G
MG
Zoccarato
F
F
Alexandre
A
A
D000255:Adenosine Triphosphate / Q000097:blood; D000535:Aluminum / Q000494:pharmacology*; D001792:Blood Platelets / Q000502:physiology; D001798:Blood Proteins / Q000378:metabolism; D006152:Cyclic GMP / Q000494:pharmacology / Q000031:analogs & derivatives; D004789:Enzyme Activation / Q000187:drug effects; D005292:Ferricyanides / Q000494:pharmacology*; D005461:Fluorine / Q000494:pharmacology*; D006162:Guanylate Cyclase / Q000378:metabolism; D006801:Humans; D009599:Nitroprusside / Q000494:pharmacology*; D010766:Phosphorylation; D015539:Platelet Activation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism; D010738:Type C Phospholipases / Q000378:metabolism
D001798:Blood Proteins; D005292:Ferricyanides; D010975:Platelet Aggregation Inhibitors; D009599:Nitroprusside; C053629:fluoroaluminum; D005461:Fluorine; C016276:8-bromocyclic GMP; D000255:Adenosine Triphosphate; D000535:Aluminum; D011493:Protein Kinase C; D010738:Type C Phospholipases; D006162:Guanylate Cyclase; D006152:Cyclic GMP