title: Beta-adrenergic responsiveness in a human submandibular tumor cell line (A253).
abstract: Salivary epithelial functions are regulated by the autonomic nervous system. In this regard, we have been studying the morphology and neuroreceptor composition of A253, an immortal cell line isolated from a human submandibular carcinoma (Giard et al., JNCI, 51:1417-1421, 1973). Phase contrast and electron microscopic observations indicate that A253 cells are of epithelial origin. Physiologically, A253 cells possess beta-adrenergic, but not alpha-adrenergic or muscarinic-cholinergic receptors. The beta-adrenergic receptors (BARs) are composed primarily of a single class of high affinity, beta 2-subtype receptors as judged by [3H]dihydroalprenolol antagonist binding studies. The BARs are functional inasmuch as isoproterenol stimulation increases both intracellular cAMP content and [3H]mannose incorporation into endogeneous glycoproteins. Differences in ultrastructure and neuroreceptor composition between A253 and other immortal salivary tumor cell lines are discussed.
authors.affiliation: Clinical Investigations and Patient Care Branch, National Institute of Dental Research, Bethesda, Maryland 20892.
authors.forename: Y
authors.initials: Y
authors.lastname: Marmary
authors.forename: X J
authors.initials: XJ
authors.lastname: He
authors.forename: A R
authors.initials: AR
authors.lastname: Hand
authors.forename: J A
authors.initials: JA
authors.lastname: Ship
authors.forename: R B
authors.initials: RB
authors.lastname: Wellner
mesh_terms: D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure; D004848:Epithelium / Q000473:pathology / Q000503:physiopathology / Q000648:ultrastructure; D006801:Humans; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast; D018377:Neurotransmitter Agents / Q000378:metabolism; D011943:Receptors, Adrenergic, beta / Q000502:physiology*; D012468:Salivary Gland Neoplasms / Q000473:pathology*; D013365:Submandibular Gland Neoplasms / Q000473:pathology* / Q000503:physiopathology / Q000648:ultrastructure; D014407:Tumor Cells, Cultured / Q000473:pathology / Q000502:physiology / Q000648:ultrastructure
chemical_list: D018377:Neurotransmitter Agents; D011943:Receptors, Adrenergic, beta