Involvement of alpha 1-adrenoceptors in the depolarizing but not the hyperpolarizing responses of motorneurones in the neonate rat to noradrenaline.
Superfusion of the hemisected lumbar spinal cord in the neonate rat with solutions containing 10(-6) to 10(-3) M noradrenaline (NA), elicited graded depolarizations recorded from ventral roots, with a mean EC50 value of 15.1 +/- 1.5 microM (mean +/- SEM, n = 37). Repeated concentration-response curves to NA could be determined from the same preparation. Adrenaline had a similar depolarizing action (EC50 9.9 +/- 1.7 microM, n = 11). Blockade of neuronal uptake of NA by desipramine (2 x 10(-6) M) caused some potentiation of submaximal responses to NA and shifted the EC50 to 6.0 +/- 1.7 microM (mean +/- SEM, n = 14). The depolarizing response to NA was unaffected by DL-propranolol (10(-7) M) or yohimbine (10(-7) M). Prazosin (5 x 10(-9), 10(-8) and 10(-7) M) reduced the responses and caused a progressive rightward shift of the concentration-response curve. The onset of blockade by prazosin was slow, superfusion for at least 90-120 min being required before the blockade plateaued. Prazosin (5 x 10(-9) and 10(-8) M) caused a surmountable blockade, the apparent pA2 being 8.3 +/- 0.2 (mean +/- SEM, N = 9). Depolarizations induced by NA were also antagonised by phentolamine (10(-6) M). An initial hyperpolarizing response to NA was unmasked after exposure to prazosin in 90% of preparations and was associated with a reduction in the spontaneous activity of the motorneurones. Both the hyperpolarization and reduction in spontaneous activity were attenuated by yohimbine.(ABSTRACT TRUNCATED AT 250 WORDS)
Connell
L A
LA
Department of Physiology, University of Wales College of Cardiff, U.K.
Majid
A
A
Wallis
D I
DI
D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D002395:Catecholamines / Q000378:metabolism; D004558:Electric Stimulation; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects; D009046:Motor Neurons / Q000187:drug effects*; D014179:Neurotransmitter Uptake Inhibitors / Q000494:pharmacology; D009638:Norepinephrine / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D011224:Prazosin / Q000494:pharmacology; D051381:Rats; D011942:Receptors, Adrenergic, alpha / Q000502:physiology*
D002395:Catecholamines; D014179:Neurotransmitter Uptake Inhibitors; D011942:Receptors, Adrenergic, alpha; D009638:Norepinephrine; D011224:Prazosin