[Use of the centrifugation-culture technique for the rapid diagnosis of cytomegalovirus].
Cell culture is the reference method for the detection of cytomegalovirus (CMV) in clinical samples. However, owing to the slow development of cytopathic effect (CPE), the cultures should be observed for at least four weeks before being discarded as negative. We have used a monoclonal antibody for the immunofluorescent detection of the early CMV antigen 48 hours after the inoculation of the samples, by means of centrifugation on embryonic human lung fibroblasts. Fifty-nine out of the 566 investigated urine samples were positive for early antigen in culture and 57 in conventional culture on the same cellular type (mean time of development of CPE 11.8 days). A modification facilitating sample processing and a considerable saving of reactive is described. The technique for the detection of early CMV antigen in culture after centrifugation can be used for the diagnosis of CMV infection owing to its high sensitivity and the rapidity of the results.
Mendoza Montero
J
J
Navarro Marí
J M
JM
de la Rosa
M
M
D000328:Adult; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology; D002478:Cells, Cultured; D002498:Centrifugation; D002648:Child; D003587:Cytomegalovirus / Q000276:immunology / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D005260:Female; D005347:Fibroblasts / Q000382:microbiology; D006801:Humans; D017874:Immediate-Early Proteins; D007231:Infant, Newborn; D008297:Male; D011237:Predictive Value of Tests; D013997:Time Factors; D014556:Urine / Q000382:microbiology; D014776:Virus Cultivation / Q000379:methods*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D017874:Immediate-Early Proteins; C044582:immediate-early proteins, cytomegalovirus
Utilización de la técnica de centrifugación-cultivo para el diagnóstico rápido de citomegalovirus.