Comparison of an antibody-capture IgM enzyme-linked immunosorbent assay with IgM-indirect immunofluorescence for the diagnosis of acute Sindbis and West Nile infections.
The development and evaluation of an antibody-capture ELISA for the detection of IgM antibodies to Sindbis (SIN) and West Nile (WN) viruses are described. Comparison of the ELISA results with those obtained by indirect immunofluorescence (IIF) antibody tests using both fluorescein and biotinylated anti-human IgM conjugates, showed that the former technique was both more sensitive and specific than the IIF methods. There were no false positives by the ELISA whereas with the IgM-IIF assays a high percentage of false positives were obtained. These were due to rheumatoid factor and also to an interfering factor which was not detected by the RF latex agglutination test. Absorption of the sera with anti-IgG was necessary to eliminate this interference in the IgM-IIF tests.
Besselaar
T G
TG
Department of Virology, University of the Witwatersrand, Johannesburg, Republic of South Africa.
Blackburn
N K
NK
Aldridge
N
N
D000914:Antibodies, Viral / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology; D011233:Precipitin Tests; D011237:Predictive Value of Tests; D012845:Sindbis Virus / Q000276:immunology; D014036:Togaviridae Infections / Q000175:diagnosis*; D014902:West Nile virus / Q000276:immunology
D000914:Antibodies, Viral; D007075:Immunoglobulin M