Examination of HeLa cell contamination of human cell lines derived from primary hepatomas using glucose-6-phosphate dehydrogenase and lactate dehydrogenase isozymes.
Isozyme patterns of glucose-6-phosphate dehydrogenase (G6PD) and lactate dehydrogenase (LDH) in human cell lines derived from primary hepatomas were compared with those in HeLa cells. Some cell lines derived from primary hepatomas having type B G6PD showed one or two isozymes of LDH. On the other hand, HeLa cells having type A G6PD showed four LDH isozymes. These findings suggest that not only G6PD, but also LDH may be useful for the detection of HeLa cell contamination of a culture in some cases.
Tokiwa
T
T
Division of Pathology, Okayama University Medical School, Japan.
Kusaka
Y
Y
Muraoka
A
A
Sato
J
J
D006528:Carcinoma, Hepatocellular / Q000201:enzymology*; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis*; D006367:HeLa Cells / Q000201:enzymology*; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D008113:Liver Neoplasms / Q000201:enzymology*; D014407:Tumor Cells, Cultured
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase