[Use of sarcoma 180 TG cells for obtaining ascitic fluid from mice hyperimmune to the foot-and-mouth disease virus].
Mice immunized with FMDV C3 Arg 84 antigen were inoculated intraperitoneally with sarcoma 180 TG. The ascitic fluid obtained by ventral puncture contained high titers of antibodies, similar to those obtained from serum, as determined by neutralization and ELISA tests. Ascitic volumes were 10 to 20 times greater than those obtainable with.
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Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias, Argentina.
Marcovecchio
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Arese
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Zuloaga
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D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D005537:Aphthovirus / Q000276:immunology*; D001202:Ascitic Fluid / Q000276:immunology*; D051379:Mice; D012510:Sarcoma 180; D014407:Tumor Cells, Cultured
D000914:Antibodies, Viral
Uso de las células de sarcoma 180 TG para la obtención de fluídos ascíticos hiperinmunes de ratón para virus aftoso.