Chromatographic method for the preparation of apo-cellular retinol-binding protein and apo-cellular retinoic acid-binding protein from their holo-types.
A chromatographic method is described for the preparation of apo-cellular retinol-binding protein (CRBP) and apo-cellular retinoic acid-binding protein (CRABP) from their corresponding holoproteins. Elimination of retinoids from either purified CRBP or CRABP holoprotein complex could be performed quantitatively by DEAE-cellulose chromatography without any alteration in the inherent properties of the native proteins. In contrast, the usual methods, involving UV irradiation or acetone precipitation, resulted in some modification of these binding proteins. This chromatographic method was also applicable to the preparation of apo-fatty acid-binding protein (FABP) from FABP-palmitic acid holoprotein complex.
Fukai
F
F
Department of Patho-Physiology, Faculty of Pharmaceutical Sciences, Science University of Tokyo, Japan.
Suzuki
H
H
Katayama
T
T
D000818:Animals; D002352:Carrier Proteins / Q000302:isolation & purification* / Q000378:metabolism; D002848:Chromatography, DEAE-Cellulose; D008297:Male; D051381:Rats; D018168:Receptors, Retinoic Acid; D012177:Retinol-Binding Proteins / Q000302:isolation & purification* / Q000378:metabolism; D054840:Retinol-Binding Proteins, Cellular; D013056:Spectrophotometry, Ultraviolet; D013737:Testis / Q000378:metabolism
D002352:Carrier Proteins; D018168:Receptors, Retinoic Acid; D012177:Retinol-Binding Proteins; D054840:Retinol-Binding Proteins, Cellular