Raman/absorption simultaneous measurements for cytochrome oxidase compound A at room temperature with a novel flow apparatus.
A novel flow apparatus for continuously producing reaction intermediates of cytochrome oxidase was constructed and applied successfully to observe the transient absorption and resonance Raman spectra in its reaction with oxygen. Time-resolved difference absorption spectra in 500-650-nm region clearly indicated the formation of compound A upon photolysis of the fully reduced CO-bound form at 5 degrees C, and at this stage electrons were not transferred from cytochrome c to cytochrome oxidase. However, at the stage of formation of compound B, cytochrome c was oxidized. Resonance Raman spectra of these intermediates measured simultaneously with the absorption spectra are also reported.
Ogura
T
T
Institute for Molecular Science, Okazaki National Research Institutes, Japan.
Yoshikawa
S
S
Kitagawa
T
T
D000042:Absorption; D000818:Animals; D002417:Cattle; D004566:Electrodes; D003576:Electron Transport Complex IV / Q000032:analysis*; D009206:Myocardium / Q000201:enzymology; D013059:Spectrum Analysis, Raman / Q000295:instrumentation; D013696:Temperature
D003576:Electron Transport Complex IV