High-performance liquid chromatography of arachidonic acid metabolites and its application to the determination of leukotriene B4 in stimulated leukocytes.
A highly sensitive high-performance liquid chromatography with fluorescence detection for the determination of arachidonic acid metabolites is described. The metabolites are converted into corresponding fluorescent derivatives by reaction with 3-bromomethyl-6,7-methylenedioxy-1-methyl-2(1H)-quinoxalinone in the presence of potassium hydrogen carbonate and 18-crown-6 in acetonitrile. The derivatives are separated on a reversed-phase column (Inertsil ODS) with aqueous acetonitrile and detected fluorimetrically. The detection limits are 5-15 fmol at a signal-to-noise ratio of 3 in a 10-microliter injection volume. The method is applied to the determination of leukotriene B4 produced in stimulated leukocytes.
Yamaguchi
M
M
Takehiro
O
O
Ishida
J
J
Nakamura
M
M
D001095:Arachidonic Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007962:Leukocytes / Q000032:analysis*; D007975:Leukotriene B4 / Q000032:analysis*
D001095:Arachidonic Acids; D007975:Leukotriene B4