Guanidine-resistant mutants of poliovirus have distinct mutations in peptide 2C.
In previous work in our laboratory, 12 guanidine-resistant (gr) mutants of poliovirus were selected from 12 separate stocks of plaque-purified guanidine-sensitive (gs) viruses (K. Anderson-Sillman, S. Bartal, and D. R. Tershak, J. Virol. 50:922-928, 1984). Peptide mapping of protein 2C and evaluation of virus growth at different temperatures enabled us to subdivide these mutants into several distinct groups (D. R. Tershak, Can. J. Microbiol. 31:1166-1168, 1985; Anderson-Sillman et al., J. Virol.). Studies by Pincus et al. indicate that two nucleotide changes in codon 179 of protein 2C leads to an Asn-to-Gly or Asn-to-Ala change and that these missense modifications account for guanidine resistance (S. E. Pincus, H. Rohl, and E. Wimmer, Virology 157:83-88, 1987; S. E. Pincus and E. Wimmer, J. Virol. 60:793-796, 1986). In the present report, we confirm their findings but also show that a single amino acid change of Phe to Tyr in residue 164 of protein 2C or a Met-to-Leu replacement in amino acid 187 coupled with mutations in codons 233 or 295 and 309 could confer guanidine resistance.
Baltera
R F
RF
Department of Molecular and Cell Biology, Pennsylvania State University, University Park 16802.
Tershak
D R
DR
D003062:Codon; D004351:Drug Resistance; D019791:Guanidine; D006146:Guanidines / Q000494:pharmacology*; D009154:Mutation; D017955:Poliovirus / Q000187:drug effects / Q000235:genetics*; D012367:RNA, Viral / Q000032:analysis; D014764:Viral Proteins / Q000235:genetics*
D003062:Codon; D006146:Guanidines; D012367:RNA, Viral; D014764:Viral Proteins; D019791:Guanidine