The effects of purine nucleotides on transmission in vesical parasympathetic ganglia of the cat.
1. In adult cats, postganglionic nerve fibres on the surface of the bladder were isolated and multiunit activity of these fibres was recorded. In these cats, the urinary bladder was cannulated and intravesical pressure was also recorded. 2. ATP, APPCP, ADP, AMP and adenosine depress transmission in vesical parasympathetic ganglia equipotently; however, 2-chloroadenosine was 10-fold more potent than ATP. 3. 2-chloroadenosine, ATP, ADP, AMP, adenosine and APPCP inhibit neurally evoked bladder contractions in the same order or potency with which they depress pelvic ganglionic transmission; however, adenine, inosine, IMP and ITP were ineffective. 4. 3',5'-cyclic AMP and dibutyryl cAMP produced little or no effect on bladder activity. 5. ATP and APPCP produced a transient rise in intravesical pressure at doses 2 to 50 times the dose needed for inhibition, presumably through ATP (P2) receptors. APPCP was 10 to 20 times more potent in exciting the bladder than ATP. 6. Theophylline and caffeine effectively antagonized purinergic effects mediated through adenosine (P1) receptors on both pelvic ganglia and bladder smooth muscle. 7. ATP inhibition of TMA-evoked bladder contractions and postganglionic nerve firing suggests that purinergic inhibition occurs, at least in part, at a postsynaptic site in the ganglia.
Theobald
R J
RJ
Department of Pharmacology, Kirksville College of Osteopathic Medicine, Missouri 63501.
de Groat
W D
WD
D000241:Adenosine / Q000494:pharmacology; D000244:Adenosine Diphosphate / Q000494:pharmacology; D000249:Adenosine Monophosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D002415:Cats; D004087:Dihydroergotamine / Q000494:pharmacology; D005260:Female; D005726:Ganglia, Parasympathetic / Q000187:drug effects*; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D011685:Purine Nucleotides / Q000494:pharmacology*; D009435:Synaptic Transmission / Q000187:drug effects*; D013806:Theophylline / Q000494:pharmacology; D001743:Urinary Bladder / Q000187:drug effects / Q000294:innervation
D011685:Purine Nucleotides; C005147:5'-adenylyl (beta,gamma-methylene)diphosphonate; D000249:Adenosine Monophosphate; D004087:Dihydroergotamine; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D011433:Propranolol; D013806:Theophylline; D000241:Adenosine; C002630:alpha,beta-methyleneadenosine 5'-triphosphate; D009638:Norepinephrine