title: The effects of citrate on hydroxyapatite induced calcium oxalate crystallization and on the formation of calcium phosphate crystals.
abstract: The addition of different amounts of hydroxyapatite crystals (HAP) to a solution, metastably supersaturated with respect to calcium oxalate (CaOx) resulted in heterogenous crystallization at seed concentrations exceeding 0.2 mmol/l. The induction period varied between 1 and more than 8 h with the shortest period for a seed concentration of 2 mmol/l. Addition to the system of 1 and 2% of whole urine and citrate in concentrations corresponding to approximately 1% of that found in normal urine inhibited the crystallization for as long as 4 h. In a system supersaturated with respect to calcium phosphate (CaP) the total number of crystals was markedly reduced by citrate concentrations exceeding 0.5 mmol/l. The fractions of medium sized and large crystals were sharply reduced and small crystals predominated at higher citrate concentrations. This might indicate effects of citrate on both crystal growth and crystal aggregation. We conclude that increased citrate concentrations during treatment with alkali leads to a significant inhibition of CaOx growth on HAP as well as to a prevention of the formation of large CaP crystals from solutions supersaturated with respect to CaP.
authors.affiliation: Department of Urology, University Hospital, Linköping, Sweden.
authors.forename: C
authors.initials: C
authors.lastname: Berg
authors.forename: H G
authors.initials: HG
authors.lastname: Tiselius
mesh_terms: D002129:Calcium Oxalate / Q000037:antagonists & inhibitors* / Q000652:urine; D002130:Calcium Phosphates / Q000037:antagonists & inhibitors* / Q000652:urine; D002951:Citrates / Q000494:pharmacology*; D019343:Citric Acid; D003460:Crystallization; D017886:Durapatite; D006801:Humans; D006882:Hydroxyapatites / Q000494:pharmacology*; D013997:Time Factors; D014545:Urinary Calculi / Q000652:urine
chemical_list: D002130:Calcium Phosphates; D002951:Citrates; D006882:Hydroxyapatites; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; D002129:Calcium Oxalate; D019343:Citric Acid; C485838:calcium phosphate, monobasic, anhydrous; D017886:Durapatite; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous