title: Distribution and developmental transition of phosphoglycerate mutase and creatine phosphokinase isozymes in rat muscles of different fiber-type composition.
abstract: Phosphoglycerate mutase and creatine phosphokinase have in mammals three isozymes (types MM, MB and BB) with similar tissue distribution and developmental transition in muscle cells. To assess whether the phenotype and the developmental switch of these isozymes differ in the diverse types of muscle fibers, the enzymatic activities and the isozyme patterns, analyzed by cellulose acetate electrophoresis, have been determined in rat soleus, extensor digitorum longus and gastrocnemius muscles during postnatal development. Both phosphoglycerate mutase and creatine phosphokinase activity increased in the three muscles, the increase in extensor digitorum longus and gastrocnemius being higher than in soleus. For the two enzymes the increase in activity was due to the progressive increment of the muscle-specific forms. It is concluded that whereas phosphoglycerate mutase and creatine phosphokinase type-B subunits are present at similar levels in both type I and type II muscle fibers, phosphoglycerate mutase and creatine phosphokinase type-M subunits exhibit much higher levels in type II fibers.
authors.affiliation: Unitat de Bioquímica, Facultat de Medicina, Universitat de Barcelona, Spain.
authors.forename: V
authors.initials: V
authors.lastname: Andrés
authors.forename: R
authors.initials: R
authors.lastname: Cussó
authors.forename: J
authors.initials: J
authors.lastname: Carreras
mesh_terms: D000818:Animals; D003402:Creatine Kinase / Q000032:analysis / Q000378:metabolism*; D004586:Electrophoresis; D007527:Isoenzymes / Q000032:analysis / Q000378:metabolism*; D008297:Male; D009132:Muscles / Q000032:analysis / Q000166:cytology / Q000201:enzymology*; D010736:Phosphoglycerate Mutase / Q000032:analysis / Q000378:metabolism*; D010770:Phosphotransferases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013053:Spectrophotometry
chemical_list: D007527:Isoenzymes; D010770:Phosphotransferases; D003402:Creatine Kinase; D010736:Phosphoglycerate Mutase