Molecular cloning, sequence and functional expression of the cDNA for the human thyrotropin receptor.
We screened a human thyroid cDNA library with two synthetic oligonucleotides based on the reported amino acid sequence of the 3rd and 4th transmembrane domains of a putative human TSH receptor and related receptors. The nucleotide sequence of a 4 kb clone revealed an open reading frame of 764 amino acids (86,816 Daltons) with a putative signal peptide, seven transmembrane domains, five potential glycosylation sites, and a very short intracytoplasmic region. Homology with the extracellular domain of the pig LH/CG receptor was only 33%. Chinese hamster ovary cells stably transfected with this cDNA in an expression vector generated a functional receptor, able to activate adenylate cyclase, specifically in response to TSH stimulation.
Nagayama
Y
Y
Thyroid Molecular Biology Laboratory, Veterans' Administration Medical Center, San Francisco, California 94121.
Kaufman
K D
KD
Seto
P
P
Rapoport
B
B
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D003062:Codon; D006224:Cricetinae; D000242:Cyclic AMP / Q000378:metabolism; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D006031:Glycosylation; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011974:Receptors, LH / Q000235:genetics; D011989:Receptors, Thyrotropin / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
D003062:Codon; D011974:Receptors, LH; D011989:Receptors, Thyrotropin; D004247:DNA; D000242:Cyclic AMP