Evidence for tight metabolic control of the receptor-activated polyphosphoinositide cycle in human platelets.
The [32P]PIP2/[32P]PA and the [32P]PIP/[32P]PA relationships were demonstrated to be remarkably similar after stimulation of [32P]Pi-prelabelled platelets for 90 s with various combinations and concentrations of agonists and inhibitors. Thus the activity of the PI and PIP kinases with the corresponding phosphomonoesterases may be tightly controlled during receptor-mediated platelet stimulation involving phospholipase C activation.
Steen
V M
VM
Department of Biochemistry, University of Bergen, Norway.
Tysnes
O B
OB
Holmsen
H
H
D000244:Adenosine Diphosphate / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D003402:Creatine Kinase / Q000097:blood; D004789:Enzyme Activation; D004837:Epinephrine / Q000494:pharmacology; D006629:Hirudins / Q000494:pharmacology; D006801:Humans; D010712:Phosphatidic Acids / Q000097:blood; D019269:Phosphatidylinositol 4,5-Diphosphate; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols / Q000097:blood*; D010725:Phosphocreatine / Q000097:blood; D010761:Phosphorus Radioisotopes; D013917:Thrombin / Q000494:pharmacology; D010738:Type C Phospholipases / Q000378:metabolism
D006629:Hirudins; D010712:Phosphatidic Acids; D019269:Phosphatidylinositol 4,5-Diphosphate; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols; D010761:Phosphorus Radioisotopes; C037178:phosphatidylinositol 4-phosphate; D010725:Phosphocreatine; D000244:Adenosine Diphosphate; D003402:Creatine Kinase; D010738:Type C Phospholipases; D013917:Thrombin; D004837:Epinephrine