Aromatase activity in cultured ovaries from fetal and neonatal golden hamsters.
The production of oestrogen from androstenedione by ovaries of hamsters on day 14 of pregnancy and on days 0 and 3 after birth was investigated by culturing these ovaries for 48 h in serum-free medium containing insulin and [3H]androstenedione. Both oestrone and oestradiol-17 beta were produced in culture of ovaries on day 14 of pregnancy. The production of oestrogen increased about 2-fold in culture of ovaries on days 0 and 3. Dibutyryladenosine 3':5'-cyclic monophosphate stimulated the oestrogen production by ovaries on day 14 of pregnancy and on days 0 and 3. The present results indicate that the ovaries of fetal and neonatal hamsters have an aromatase activity and that these aromatase activities are responsive to adenosine 3':5'-cyclic monophosphate.
Terada
N
N
Department of Experimental Pathology, Osaka University Medical School, Japan.
Takada
T
T
Yamamoto
R
R
Taniguchi
H
H
Takatsuka
D
D
Li
W
W
Matsumoto
K
K
D000818:Animals; D000831:Animals, Newborn / Q000502:physiology; D001141:Aromatase / Q000378:metabolism*; D002478:Cells, Cultured; D006224:Cricetinae; D000242:Cyclic AMP / Q000494:pharmacology; D005260:Female; D005333:Fetus / Q000166:cytology / Q000201:enzymology; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D008647:Mesocricetus; D010053:Ovary / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*
D005640:Follicle Stimulating Hormone; D000242:Cyclic AMP; D001141:Aromatase