Essential fatty acid deficiency inhibits early but not late leukocyte infiltration in rabbit myocardial infarcts.
Essential fatty acid (EFA) deficiency, induced by elimination of the dietary (n-6) fatty acids, has been shown to limit inflammatory cell influx and consequent enhanced eicosanoid production in experimental glomerulonephritis and hydronephrosis. To determine whether EFA-deficiency exerts anti-inflammatory effects following left ventricular myocardial infarction (LVMI), male weanling rabbits were fed EFA-deficient diet for 3 months prior to 60 minutes of distal left circumflex coronary artery occlusion followed by reperfusion. One and 4 days later, corresponding to infiltration of cardiac tissue with polymorphonuclear (PMN) and mononuclear leukocytes respectively, infarcted hearts were buffer perfused and stimulated to produce eicosanoids with f-met-leu-phe or bradykinin. One day following LVMI, the hearts of EFA-deficient rabbits demonstrated a marked suppression of PMN infiltration and eicosanoid production relative to controls. Four days following myocardial infarction, no differences were observed in mononuclear cell invasion, collagen deposition, or eicosanoid production between EFA-deficient and normal hearts. Our data show that EFA-deficiency inhibits PMN influx and consequent enhanced eicosanoid production without affecting the later appearance of mononuclear cells, collagen deposition, or eicosanoid production. Recent studies have shown that suppression of PMN invasion limits the extent of tissue damage following LVMI. Selective inhibition of PMN infiltration is possible and may be useful in the management of acute myocardial infarction.
Freed
M S
MS
Dept. of Pharmacology, Washington University School of Medicine, St. Louis, MO 63110.
Spaethe
S M
SM
Lefkowith
J B
JB
Saffitz
J E
JE
Needleman
P
P
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis; D000210:Acute-Phase Reaction; D000818:Animals; D002464:Cell Migration Inhibition; D003094:Collagen / Q000378:metabolism; D015232:Dinoprostone / Q000096:biosynthesis; D005228:Fatty Acids, Essential / Q000172:deficiency*; D007249:Inflammation; D007975:Leukotriene B4 / Q000096:biosynthesis; D008297:Male; D009203:Myocardial Infarction / Q000503:physiopathology*; D009206:Myocardium / Q000473:pathology; D009504:Neutrophils / Q000276:immunology*; D011817:Rabbits; D013929:Thromboxane B2 / Q000096:biosynthesis
D005228:Fatty Acids, Essential; D007975:Leukotriene B4; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D003094:Collagen; D015232:Dinoprostone