title: In vitro effects of ketoconazole on corticotrope cell morphology and ACTH secretion of two pituitary adenomas removed from patients with Nelson's syndrome.
abstract: The direct effects of ketoconazole on the secretion of ACTH by human pituitary adenoma cells from 2 patient with Nelson's syndrome were studied in vitro. Stereologically quantified, intracellular changes affect the surface density of the endoplasmic reticulum (it decreased by 73%), the volume density of the secretion granules (it decreased by 49%), and the volume density of lysosomes (it decreased by 67%). The hormone released in the culture medium decreased depending on the doses of ketoconazole used; 10 mumol/l decreased ACTH levels by 31%. These data show that ketoconazole induce marked changes on corticotrope morphology and ACTH secretion in pituitary cells obtained from patients with Nelson's syndrome.
authors.affiliation: Dpto. Ciencias Morfolgicas, Universidad de Cördoba, Sevilla, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Jimenez Reina
authors.forename: A
authors.initials: A
authors.lastname: Leal-Cerro
authors.forename: J
authors.initials: J
authors.lastname: Garcia
authors.forename: P P
authors.initials: PP
authors.lastname: Garcia-Luna
authors.forename: R
authors.initials: R
authors.lastname: Astorga
authors.forename: G
authors.initials: G
authors.lastname: Bernal
mesh_terms: D000236:Adenoma / Q000378:metabolism* / Q000648:ultrastructure; D000324:Adrenocorticotropic Hormone / Q000378:metabolism*; D000328:Adult; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007654:Ketoconazole / Q000494:pharmacology*; D009347:Nelson Syndrome / Q000378:metabolism* / Q000648:ultrastructure; D010902:Pituitary Gland / Q000187:drug effects*; D010911:Pituitary Neoplasms / Q000378:metabolism*
chemical_list: D000324:Adrenocorticotropic Hormone; D007654:Ketoconazole