Proton efflux from rat intestinal basal-lateral membrane vesicles is stimulated by ATP and Na+.
1. ATP-stimulated 22Na uptake and 14C-methylamine efflux were studied in inside-out rat intestinal basal-lateral membrane vesicles (BLMV). 2. Uptake of 22Na by basal-lateral membrane vesicles was stimulated by addition of ATP and by an acidic vesicle interior. 3. Efflux of 14C-methylamine was stimulated by ATP and Na+. 4. 14C-methylamine efflux was not influenced by vanadate or amiloride by themselves but was inhibited by the presence of both agents. 5. These data are consistent with a basal-lateral proton translocation mechanism which may be responsible for alkalinization of the lateral intercellular space and implicates the Na+-pump in this mechanism.
Moe
A J
AJ
George Washington University Medical Center, Washington, DC 20037.
Hollywood
J A
JA
Jackson
M J
MJ
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D001693:Biological Transport, Active; D002458:Cell Fractionation / Q000379:methods; D002462:Cell Membrane / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000502:physiology* / Q000648:ultrastructure; D008297:Male; D008744:Methylamines / Q000378:metabolism; D051381:Rats; D012964:Sodium / Q000378:metabolism / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D014639:Vanadium / Q000494:pharmacology
D008744:Methylamines; D014639:Vanadium; D000255:Adenosine Triphosphate; D012964:Sodium; C027451:methylamine; D000254:Sodium-Potassium-Exchanging ATPase