title: Toxic and DNA-damaging activities of the fungicides mancozeb and thiram (TMTD) on human lymphocytes in vitro.
abstract: The cytotoxic and mutagenic effects of the fungicides mancozeb and thiram were studied using human peripheral blood lymphocytes cultured in vitro with or without an S-9 mix microsomal metabolizing system. The results obtained suggested that the chemicals caused dose-dependent inhibition of thymidine uptake and unscheduled DNA synthesis on both resting and proliferating lymphocytes in the absence of the S-9 mix. In the presence of the S-9 mix, only thiram showed mutagenic activity by eliciting unscheduled DNA synthesis and a significantly higher frequency of sister chromatid exchanges than did controls.
authors.affiliation: Institute for Cancer Research, University of Bologna, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Perocco
authors.forename: M A
authors.initials: MA
authors.lastname: Santucci
authors.forename: A
authors.initials: A
authors.lastname: Gasperi Campani
authors.forename: G C
authors.initials: GC
authors.lastname: Forti
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004249:DNA Damage; D004261:DNA Replication / Q000187:drug effects; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D008344:Maneb / Q000633:toxicity*; D009153:Mutagens; D012854:Sister Chromatid Exchange / Q000187:drug effects; D013859:Thiocarbamates / Q000633:toxicity*; D013893:Thiram / Q000633:toxicity*; D015038:Zineb / Q000633:toxicity*
chemical_list: D009153:Mutagens; D013859:Thiocarbamates; D013893:Thiram; D008344:Maneb; C013099:mancozeb; D015038:Zineb