title: Porcine adrenal prolactin receptors: characterization, changes during neonatal development and effects of hypoprolactinemia.
abstract: 1. Adrenal prolactin (PRL) receptors were identified within the adrenal cortex of pigs (Sus domesticus), and found to be located specifically on isolated zona fasciculata/reticularis cells (6437 sites per cell). 2. These PRL receptors were associated with binding to [125I]-oPRL which was characterized as being time and temperature dependent, specific for PRL, saturable, of high affinity (Ka = 10(10)/M) with a single class of binding sites, and irreversible except under extreme conditions. 3. The concentrations (fmol/mg protein) of PRL receptors decreased by 35% (P less than 0.05) between 3 and 10 days of age, and subsequently remained constant until 30 days of age. Total content (fmol/paired adrenals) increased progressively (2-fold, P less than 0.05) between 3 and 30 days of age. 4. Short-term (less than 16 hr) and prolonged (7 weeks) hypoprolactinemia (46-64% of control levels, P less than 0.05) were not associated with changes in numbers of porcine adrenal unoccupied PRL receptors.
authors.affiliation: US Department of Agriculture, US Meat Animal Research Center, Clay Center, NE 68933.
authors.forename: H G
authors.initials: HG
authors.lastname: Klemcke
authors.forename: W G
authors.initials: WG
authors.lastname: Pond
authors.forename: J A
authors.initials: JA
authors.lastname: Nienaber
mesh_terms: D000302:Adrenal Cortex / Q000378:metabolism; D000311:Adrenal Glands / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development / Q000378:metabolism*; D003080:Cold Temperature; D008297:Male; D008426:Maternal Deprivation; D011388:Prolactin / Q000097:blood*; D011981:Receptors, Prolactin / Q000378:metabolism*; D013312:Stress, Physiological / Q000378:metabolism; D013552:Swine
chemical_list: D011981:Receptors, Prolactin; D011388:Prolactin