Modulation of sodium current kinetics by chlorpromazine in freshly-isolated striatal neurones of the adult guinea-pig.
1. The neurones of the striatum were freshly dissociated from the adult guinea-pig brain by enzymatic and mechanical treatments. Sodium channel current kinetics in these neurones were measured using a whole cell variation of the patch-clamp technique. 2. Chlorpromazine, a neuroleptic, in micromolar concentrations reversibly reduced the amplitude of the sodium currents. Activation and inactivation time constants were not affected. The inhibition followed one-to-one binding stoichiometry. 3. The concentration-response curve shifted to the left when the holding potential was less negative. The EC50 shifted from 4.8 microM to 0.9 microM when the holding potential was changed from -120 mV to -70 mV. 4. The steady-state activation curve of the sodium current was not affected by chlorpromazine, whereas the steady-state inactivation curve was shifted in the negative direction. Consequently, the window current which is normally present at a potential range around -50 mV was decreased in the presence of chlorpromazine. 5. Successive sodium currents evoked by a train of depolarizing pulses (30 ms duration) to -10 mV showed a cumulative decrease in size during the application of chlorpromazine. However, such 'use-dependent' block was not observed when the pulse duration was reduced to 1 ms. 6. The recovery from inactivation in the presence of chlorpromazine, was expressed as a second order process. The faster component was similar to the recovery time course of the normal sodium channels. The slower component accounted for the use-dependent effect of chlorpromazine. 7. The results indicate that chlorpromazine binds to the resting sodium channels producing steady-state block at a very negative holding potential. When the membrane is depolarized, chlorpromazine binds to the inactivated form of the sodium channels with much higher affinity and stabilizes them in the inactivated state, slowing their kinetics.
Ogata
N
N
Department of Pharmacology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Tatebayashi
H
H
D000818:Animals; D002746:Chlorpromazine / Q000494:pharmacology*; D003342:Corpus Striatum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004594:Electrophysiology; D005260:Female; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D015222:Sodium Channels / Q000187:drug effects*
D015222:Sodium Channels; D002746:Chlorpromazine