[Role of liposomes in the presentation of HIV envelope glycoprotein and the immune response in mice].
The role of antigen presentation in the induction of humoral as well as cell-mediated immune responses has been investigated by anchoring HIV-1 envelope glycoprotein (gp 160/120) into the phospholipidic bilayer of preformed liposomes to produce HIV-Immunosomes. HIV-Immunosomes induced high titres of HIV-specific antibodies when tested by ELISA, IFA and neutralization, whereas equal amounts of purified glycoprotein alone produced lower antibody response. Similarly, HIV-Immunosomes induced antigen-specific Interleukin-2 production and blastogenic response upon restimulation with the same antigen, in animals vaccinated with HIV-Immunosomes, whereas no secondary response was observed in animals vaccinated with equal amounts of purified gp 160/120. Taken together, these results underline the importance of antigen presentation in the establishment of an adequate immune status and show the potential of HIV-Immunosomes as vaccine against AIDS.
Thibodeau
L
L
Institut Armand-Frappier, Université du Québec, Laval, P.Q., Canada.
Chagnon
M
M
Flamand
L
L
Oth
D
D
Lachapelle
L
L
Tremblay
C
C
Montagnier
L
L
D000818:Animals; D000917:Antibody Formation; D004337:Drug Carriers; D015686:Gene Products, env / Q000008:administration & dosage / Q000276:immunology; D015699:HIV Envelope Protein gp120 / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D007111:Immunity, Cellular; D008081:Liposomes / Q000276:immunology*; D051379:Mice
D004337:Drug Carriers; D015686:Gene Products, env; D015699:HIV Envelope Protein gp120; D008081:Liposomes
Rôle des liposomes dans la présentation de la glycoprotéine de l'enveloppe du VIH et la réponse immunitaire chez la souris.