Primary cells and established cell lines join DNA ends with the same efficiency relative to homologous recombination.
The joining of DNA ends was compared in the established CV1 cell line and in African green monkey kidney primary cells, from which the CV1 cell line was established. Using a linear SV40 genome that carries a terminal repeat of 526 nucleotide pairs, we measured the efficiency of circularization by end joining relative to circularization by homologous recombination between the terminal repeats. The ratios of end joining to homologous recombination were identical in the two types of cells regardless of whether the DNA ends were sticky, blunt, or mismatched. These studies demonstrate that the efficient end joining observed in established cell lines is not a peculiar adaptation to life in culture, but rather reflects a normal aspect of DNA metabolism in mammalian cells. Furthermore, the similar results in primary and established cells support the general validity of using data from studies of cultured cells to formulate models of recombinational processes in primary cells and potentially in multicellular organisms as well.
Zheng
H
H
Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030.
Chang
X B
XB
Wilson
J H
JH
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D004247:DNA / Q000235:genetics*; D005818:Genetic Engineering; D010957:Plasmids; D011995:Recombination, Genetic; D012107:Research Design; D013539:Simian virus 40 / Q000235:genetics; D014162:Transfection
D004247:DNA