title: Functional significance of phosphorylation to the human immunodeficiency virus Rev protein.
abstract: The human immunodeficiency virus Rev protein is posttranslationally modified by a serine kinase activity present in the nucleus of the cell. Site-directed mutagenesis was used to identify the site of phosphorylation. Changing of serine residues 92 and 99 dramatically reduced Rev phosphorylation, suggesting that at least one, if not both, of these residues is the one recognized by the Rev-specific serine kinase. Similarly, a truncated Rev protein lacking the 25 carboxy-terminal amino acids was not phosphorylated. By using two independent assays, both the serine mutant proteins and the truncated form of Rev were found to be fully functional. Thus, phosphorylation and the 25 carboxy-terminal amino acids appear to be dispensable for protein function.
authors.affiliation: Department of Molecular Oncology, Roche Institute of Molecular Biology, Nutley, New Jersey 07110.
authors.forename: A W
authors.initials: AW
authors.lastname: Cochrane
authors.forename: E
authors.initials: E
authors.lastname: Golub
authors.forename: D
authors.initials: D
authors.lastname: Volsky
authors.forename: S
authors.initials: S
authors.lastname: Ruben
authors.forename: C A
authors.initials: CA
authors.lastname: Rosen
mesh_terms: D015696:Gene Products, tat; D006678:HIV / Q000235:genetics*; D009856:Oncogene Proteins, Viral / Q000502:physiology*; D010766:Phosphorylation; D014157:Transcription Factors / Q000502:physiology*; D054322:tat Gene Products, Human Immunodeficiency Virus
chemical_list: D015696:Gene Products, tat; D009856:Oncogene Proteins, Viral; D014157:Transcription Factors; D054322:tat Gene Products, Human Immunodeficiency Virus