Evaluation of a mouse Y chromosome probe for assessing marrow transplantation.
The multicopy mouse Y chromosome DNA probe 80Y/B (1) has been used to probe genomic DNA isolated from male and female bone marrow cells, mixed together in known ratios. It was found that in a mixture with a ratio of 1 male:200 female marrow cells the male cells could readily be detected by this method. The potential use of this technique for assessing repopulation kinetics of marrow transplantation is discussed.
Hampson
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Department of Experimental Haematology, Paterson Institute for Cancer Research, Manchester, England.
Spooncer
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Dexter
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TM
D000818:Animals; D001853:Bone Marrow / Q000032:analysis; D016026:Bone Marrow Transplantation; D002678:Chimera; D015342:DNA Probes; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000032:analysis; D008297:Male; D051379:Mice; D009693:Nucleic Acid Hybridization; D014998:Y Chromosome
D015342:DNA Probes