Cigarette smoke impairment of human lymphocyte function by inhibition of transglutaminase.
The in-vitro and in-vivo effects of cigarette smoke were studied in human peripheral blood lymphocytes by applying a method for the capping of beta 2-microglobulin- or phytohaemagglutinin (PHA)-stimulated lymphocyte transformation (measured as (3H)thymidine incorporation) involving the transglutaminase pseudosubstrate monodansylthiacadaverine (MDTC), whose presence resulted in significantly reduced capping and (3H)thymidine incorporation in a concentration-dependent manner. The addition of dimethyl sulphoxide-soluble particles from cigarette smoke to lymphocytes in vitro significantly reduced the capping ability and the PHA-induced (3H)thymidine incorporation. Whereas no significant change in MDTC-dependent capping inhibition was seen in lymphocytes from smokers after 10 d abstinence from smoking. there was a marked decrease in (3H)thymidine incorporation in lymphocytes from smokers after smoking three cigarettes following 10 h abstinence. The tentative conclusion is that exposure to cigarette smoke, or smoke extract, impairs MDTC-dependent capping inhibition and PHA-stimulated lymphocyte transformation by transglutaminase inhibition.
Berntorp
K
K
Department of Internal Medicine, University of Lund, Malmö General Hospital, Sweden.
Ekman
M
M
Berntorp
E
E
D000328:Adult; D002103:Cadaverine / Q000031:analogs & derivatives / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000032:analysis; D005260:Female; D006801:Humans; D007152:Immunologic Capping; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D010835:Phytohemagglutinins / Q000494:pharmacology; D012906:Smoke / Q000009:adverse effects*; D012907:Smoking / Q000009:adverse effects*; D011503:Transglutaminases / Q000378:metabolism*
D010835:Phytohemagglutinins; D012906:Smoke; C053510:monodansylthiacadaverine; D011503:Transglutaminases; D002103:Cadaverine; D004121:Dimethyl Sulfoxide