Transformation of NIH3T3 cells by A-MuLV proviral DNA: effect of plasmid linearization and carrier DNA on transformation efficiency.
We have optimized the conditions for efficient NIH3T3 focus formation by calcium phosphate transfection of proviral Abelson-murine leukemia virus (A-MuLV) plasmid DNA. Linearized pA-MuLV, P120 or P160 strains, when transfected with calf thymus carrier DNA, will produce 40-50 foci/100 ng pA-MuLV without co-transfecting Moloney-murine leukemia virus (Mo-MuLV) plasmid DNA.
Shore
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SK
Wistar Institute, Philadelphia, Pennsylvania 19104.
Reddy
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EP
D000011:Abelson murine leukemia virus / Q000235:genetics*; D000818:Animals; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D004247:DNA / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D009052:Leukemia Virus, Murine / Q000235:genetics*; D051379:Mice; D008815:Mice, Inbred Strains; D010957:Plasmids; D011533:Proviruses / Q000235:genetics*; D012474:Salmon; D014162:Transfection
D004279:DNA, Viral; D004247:DNA