Bovine leucosis virus challenge infection of calves following application of BL-3 cells.
8 calves were vaccinated 3 times with 1.5.10(7) BL-3 cells. 2 and 6 weeks, respectively, after the 3rd vaccination each 4 calves were subjected to a challenge infection with 2.5.10(5) blood lymphocytes of a BLV-infected cow. For control a challenge infection was performed on 3 non-vaccinated calves. The vaccination caused in all calves the appearance of complement-dependent cytotoxic antibodies against BL-3 cells but not of antibodies against BLV-gp51. After the challenge infection there occurred in the sera of all vaccinated calves and of all non-vaccinated control animals antibodies against gp51, and BLV could be demonstrated in the cultivated blood lymphocytes of all animals. Consequently, the application of BL-3 cells had no protective effect against the BLV infection.
Ristau
E
E
Wittmann
W
W
Starick
E
E
Kluge
K H
KH
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control*; D007114:Immunization / Q000662:veterinary*; D001909:Leukemia Virus, Bovine / Q000276:immunology*; D007942:Leukemia, Experimental / Q000517:prevention & control / Q000662:veterinary*; D008214:Lymphocytes / Q000276:immunology*; D012190:Retroviridae / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000914:Antibodies, Viral