Molecular cloning and expression of a locus (mdoA) implicated in the biosynthesis of membrane-derived oligosaccharides in Escherichia coli.
Mutants of Escherichia coli defective in the mdoA locus are blocked at an early stage in the biosynthesis of membrane-derived oligosaccharides. The mdoA locus has now been cloned into multicopy plasmids. A 5 kb DNA fragment is necessary to complement mdoA mutations. Cells harbouring the mdoA+ plasmid produced three to four times more MDO than wild-type cells. MDO overproduction did not affect the degree of MDO substitution with sn-1-phosphoglycerol residues. The biosynthesis of MDO remained under osmotic control in overproducing strains.
Lacroix
J M
JM
Institut de Microbiologie, URA 136 CNRS, Université Paris-Sud, Orsay, France.
Tempête
M
M
Menichi
B
B
Bohin
J P
JP
D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D008566:Membranes / Q000378:metabolism; D009154:Mutation; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000235:genetics; D009997:Osmotic Pressure; D010957:Plasmids; D015183:Restriction Mapping; D014161:Transduction, Genetic
D004251:DNA Transposable Elements; D009844:Oligosaccharides