Stimulation of dopamine release in the rat neostriatum in vivo by activation of the voltage-sensitive sodium channel by scorpion venom neurotoxin.
Scorpion venom neurotoxins open sodium channels and thus may enhance neurotransmitter release by increasing membrane permeability to sodium. This study carried out in vivo examined the effects of the scorpion Androctonus australis neurotoxin (ScAaTx) on the levels of dopamine (DA) in push-pull perfusates of the striatum of chloral hydrate-anaesthetised rats. ScAaTx (2.5, 5.0 and 10.0 ng/microliters) stimulated DA release in a dose-dependent manner. The release of DA induced by ScAaTx (10 ng/microliters) was completely blocked when the brain site was perfused with Ca2+-free CSF containing 2 mM EGTA or in the presence of TTX (10(-5) M). These results indicate that the potent stimulatory effects of ScAaTx on neostriatal DA release in vivo are mediated via voltage-sensitive sodium channels.
Sirinathsinghji
D J
DJ
Department of Neuroendocrinology, AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, U.K.
Heavens
R P
RP
Sikdar
S K
SK
D000818:Animals; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D004298:Dopamine / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012604:Scorpion Venoms / Q000494:pharmacology*; D015222:Sodium Channels / Q000187:drug effects / Q000502:physiology*
D012604:Scorpion Venoms; D015222:Sodium Channels; D004298:Dopamine