[Distribution and lateral mobility of concanavalin A receptors in the course of cell cycle of human gastric cancer cells].
The distribution and lateral mobility of Con A receptors in the course of the cell cycle has been studied on human gastric low-differentiated mucous adenocarcinoma cells (MGC 80-3). Synchronized MGC 80-3 cells in monolayer were labeled with F-ConA in the different phases of cell cycle. The discontinuous pattern of surface labelling was present during G1, S and G2, while there were marked differences among them. The cells in mitosis displayed uniform distribution of Con A receptors complex with strong fluorescence. The fluorescence photobleaching recovery method has been used to determine the lateral mobilities of Con A receptors complex. There was marked difference among various phases for mobility pattern and diffusion coefficient. The pattern of mobility of Con A receptor complex was appeared diffusion mobility in M and G1 phases, while flow pattern occurred in S and G2 phases. The diffusion coefficient (D) G1 greater than M, the rate of flow S greater than G2, but mobile fraction in G2 phase is higher than other phases. These results showed that dynamic properties of Con A receptor are apparently different which were regulated by the cell cycle.
Wang
D S
DS
Liang
Y Y
YY
Shi
Y J
YJ
D002288:Adenocarcinoma, Mucinous / Q000378:metabolism* / Q000473:pathology; D002453:Cell Cycle; D002462:Cell Membrane / Q000378:metabolism; D006801:Humans; D011952:Receptors, Concanavalin A / Q000378:metabolism* / Q000502:physiology; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000473:pathology
D011952:Receptors, Concanavalin A