Effect of porcine brain natriuretic peptide (pBNP) on human adrenocortical steroidogenesis.
Porcine brain natriuretic peptide (pBNP), purified from porcine brain, had a significant suppressive effect on aldosterone and cortisol secretions in ACTH-treated cultured human adrenal cells. Concomitantly, the intracellular cGMP formation was enhanced by pBNP treatment. A specific pBNP receptor was identified in the human adrenal tissues. Affinity labelling of 125I-pBNP showed two separate molecular weights of specific binding sites for pBNP of 140 and 67 kDa. A 125I-pBNP binding study of the human adrenal membrane fraction demonstrated the presence of high-affinity and low-capacity binding sites for pBNP. Moreover, these binding sites for 125I-pBNP were displaced by unlabelled alpha-rANP as well as pBNP. From these studies, we concluded that pBNP had suppressive effects on human adrenocortical steroidogensis, possibly via a receptor which may be shared with ANP.
Hashiguchi
T
T
Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Higuchi
K
K
Ohashi
M
M
Takayanagi
R
R
Matsuo
H
H
Nawata
H
H
D000302:Adrenal Cortex / Q000187:drug effects*; D000305:Adrenal Cortex Hormones / Q000096:biosynthesis*; D000345:Affinity Labels; D000450:Aldosterone / Q000378:metabolism; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D006801:Humans; D006854:Hydrocortisone / Q000378:metabolism; D066298:In Vitro Techniques; D011956:Receptors, Cell Surface / Q000378:metabolism*
D000305:Adrenal Cortex Hormones; D000345:Affinity Labels; D011956:Receptors, Cell Surface; D000450:Aldosterone; D009320:Atrial Natriuretic Factor; D006854:Hydrocortisone