Is the profile of binding of a panel of neoglycoproteins useful as a diagnostic marker in human lung cancer?
Histomorphological evaluation of the profile of carbohydrate-binding proteins with specificities to alpha- and beta-glucosides (maltose, cellobiose), alpha-L-fucosides (L-fucose), alpha-D-mannosides (D-mannose), a N-acetylated sugar, present in natural glycoconjugates (N-acetyl-D-glucosamine), two negatively charged sugars (glucuronic acid and sialic acid), rhamnose (6-deoxy-L-mannose) or to heparin has been assessed. 133 cases of formalin fixed, paraffin embedded human lung carcinoma specimens (45 cases of epidermoid carcinoma, 24 cases of small cell anaplastic carcinoma, 48 cases of adenocarcinoma, and 16 cases of large cell anaplastic carcinoma) as well as 14 cases with epithelial or biphasic mesothelioma and 7 cases of pleuritis carcinomatosa were incubated with biotinylated, suitably modified neoglycoproteins or heparin for detecting the corresponding endogenous sugar receptors (lectins). Histochemical reaction was demonstrated by use of the avidin-biotin-method. Significant differences were obtained between the different markers used for each cell type as well as between the different cell types. For separating small cell anaplastic carcinoma from non small cell anaplastic carcinoma the markers carrying the histochemically crucial maltose, fucose, and mannose residues are useful, showing a positive reaction to only 4-8% of the small cell anaplastic carcinoma cases compared to 60-80% of the non small cell carcinoma cases. For separation of mesothelioma from pleuritis carcinomatosa a carrier, modified by attachment of N-acetylglucosamine moieties is the most useful marker being negative in all cases of the analyzed mesothelioma cases and being positive in 62-80% of the adenocarcinoma cases.(ABSTRACT TRUNCATED AT 250 WORDS)
Kayser
K
K
Department of Pathology, Thoraxklinik Heidelberg-Rohrbach, FRG.
Heil
M
M
Gabius
H J
HJ
D014408:Biomarkers, Tumor / Q000032:analysis*; D001710:Biotin; D002277:Carcinoma / Q000032:analysis* / Q000175:diagnosis / Q000378:metabolism; D006023:Glycoproteins / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D037102:Lectins / Q000378:metabolism; D008175:Lung Neoplasms / Q000032:analysis* / Q000175:diagnosis / Q000378:metabolism; D008654:Mesothelioma / Q000032:analysis* / Q000175:diagnosis / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism
D014408:Biomarkers, Tumor; D006023:Glycoproteins; D037102:Lectins; D011956:Receptors, Cell Surface; D001710:Biotin