N-myc activation by proviral insertion in MCF 247-induced murine T-cell lymphomas.
N-myc proto-oncogene rearrangement was found in three out of six AKR murine T-cell lymphomas induced by the highly oncogenic MCF 247 MuLV. Molecular analyses showed that structural modification of the proto-oncogene in all three lymphomas was in the consequence of MCF 247 proviral integration within the gene III exon. All integrated proviruses have the same transcriptional orientation as the N-myc gene. As a consequence of proviral insertion, the N-myc gene becomes transcriptionally active, producing an abnormal mRNA. These findings suggest a possible causative role of such an integrative event in murine T-cell lymphomagenesis.
Dolcetti
R
R
Experimental Oncology 1, Centro di Riferimento Oncologico, Aviano, Italy.
Rizzo
S
S
Viel
A
A
Maestro
R
R
De Re
V
V
Feriotto
G
G
Boiocchi
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002472:Cell Transformation, Viral; D004273:DNA, Neoplasm / Q000235:genetics; D015321:Gene Rearrangement; D009052:Leukemia Virus, Murine; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D011995:Recombination, Genetic; D015183:Restriction Mapping; D013601:T-Lymphocytes; D014158:Transcription, Genetic
D004273:DNA, Neoplasm; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D012334:RNA, Neoplasm