Elastin binds to a multifunctional 67-kilodalton peripheral membrane protein.
Elastin binding proteins from plasma membranes of elastin-producing cells were isolated by affinity chromatography on immobilized elastin peptides. Three proteins of 67, 61, and 55 kDa were released from the elastin resin by guanidine/detergent, soluble elastin peptides, synthetic peptide VGVAPG, or galactoside sugars, but not by synthetic RGD-containing peptide or sugars not related to galactose. All three proteins incorporated radiolabel upon extracellular iodination and contained [3H]leucine following metabolic labeling, confirming that each is a synthetic product of the cell. The 67-kDa protein could be released from the cell surface with lactose-containing buffers, whereas solubilization of the 61- and 55-kDa components required the presence of detergent. Although all three proteins were retained on elastin affinity columns, the 61- and 55-kDa components were retained only in the presence of 67-kDa protein, suggesting that the 67-kDa protein binds elastin and the 61- and 55-kDa proteins bind to the 67-kDa protein. We propose that the 67-, 61-, and 55-kDa proteins constitute an elastin-receptor complex that forms a transmembrane link between the extracellular matrix and the intracellular compartment.
Mecham
R P
RP
Department of Medicine, Jewish Hospital, Washington University Medical Center, St. Louis, Missouri 63110.
Hinek
A
A
Entwistle
R
R
Wrenn
D S
DS
Griffin
G L
GL
Senior
R M
RM
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002356:Cartilage / Q000378:metabolism*; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D002633:Chemotaxis; D002846:Chromatography, Affinity; D004549:Elastin / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D007700:Kinetics; D008023:Ligaments, Articular / Q000378:metabolism* / Q000502:physiology; D008565:Membrane Proteins / Q000378:metabolism*; D008970:Molecular Weight; D010446:Peptide Fragments; D011956:Receptors, Cell Surface / Q000302:isolation & purification / Q000378:metabolism*
D000596:Amino Acids; D008565:Membrane Proteins; D010446:Peptide Fragments; D011956:Receptors, Cell Surface; C054901:elastin-binding proteins; D004549:Elastin