Localization of the luminol-dependent chemiluminescence reaction in human granulocytes.
The granulocyte luminol-dependent chemiluminescence (CL) reaction is linked to the enzyme myeloperoxidase reacting with products of the respiratory burst activation. The results presented in this paper, show that the light generated in granulocytes originate both from intracellular and extracellular reactions; however, depending on the stimulus used the one or the other will dominate the activity measured. Furthermore, lysosomal fusion is proposed to be required for the intracellular CL reaction.
Dahlgren
C
C
Department of Medical Microbiology, University of Linköping, Sweden.
Follin
P
P
Johansson
A
A
Lock
R
R
Orselius
K
K
D000328:Adult; D001386:Azides / Q000494:pharmacology; D006098:Granulocytes / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D015759:Ionomycin / Q000494:pharmacology; D008163:Luminescent Measurements; D008165:Luminol; D010100:Oxygen / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D011724:Pyridazines; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
D001386:Azides; D011724:Pyridazines; D015759:Ionomycin; D008165:Luminol; D009195:Peroxidase; D010100:Oxygen