title: Structure of the gene of tum- transplantation antigen P91A: the mutated exon encodes a peptide recognized with Ld by cytolytic T cells.
abstract: Mutagen treatment of mouse P815 tumor cells produces immunogenic mutants that express new transplantation antigens (tum- antigens) recognized by cytolytic T cells. We found that the gene conferring expression of tum- antigen P91A contains 12 exons, encoding a 60 kd protein lacking a typical N-terminal signal sequence. The sequence shows no significant similarity with sequences in current data bases. A mutation that causes expression of the antigen is located in exon 4; it is the only apparent difference between the normal and the antigenic alleles. A short synthetic peptide corresponding to a region of exon 4 located around this mutation makes P815 cells sensitive to lysis by anti-P91A cytolytic T cells. The mutation creates a strong aggretope enabling the peptide to bind the H-2 Ld molecule. Several secondary tumor cell variants that no longer express tum- antigen P91A were found to carry deletions in the gene.
authors.affiliation: Ludwig Institute for Cancer Research, Brussels, Belgium.
authors.forename: C
authors.initials: C
authors.lastname: Lurquin
authors.forename: A
authors.initials: A
authors.lastname: Van Pel
authors.forename: B
authors.initials: B
authors.lastname: Mariamé
authors.forename: E
authors.initials: E
authors.lastname: De Plaen
authors.forename: J P
authors.initials: JP
authors.lastname: Szikora
authors.forename: C
authors.initials: C
authors.lastname: Janssens
authors.forename: M J
authors.initials: MJ
authors.lastname: Reddehase
authors.forename: J
authors.initials: J
authors.lastname: Lejeune
authors.forename: T
authors.initials: T
authors.lastname: Boon
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D001483:Base Sequence; D002460:Cell Line; D005091:Exons; D014644:Genetic Variation; D006183:H-2 Antigens / Q000276:immunology*; D006649:Histocompatibility Antigens / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D013489:Suppression, Genetic; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000276:immunology / Q000378:metabolism
chemical_list: D000951:Antigens, Neoplasm; D006183:H-2 Antigens; D006649:Histocompatibility Antigens; C070850:transplantation antigen P91A, mouse