[Immobilized oligonucleotides as affinity sorbents for restriction endonucleases].
Affinity chromatography of IIS type restriction endonucleases is proposed. It is shown that endonucleases HgaI, FokI, and SfaNI have affinity to the matrix with immobilized oligonucleotides which contain the endonuclease's recognition sites resistant to the hydrolysis.
Degtiarev
S Kh
SKh
Belavin
P A
PA
Shishkina
I G
IG
Zarytova
V F
VF
Gavriuchenkova
L P
LP
D001483:Base Sequence; D002846:Chromatography, Affinity; D004262:DNA Restriction Enzymes; D004587:Electrophoresis, Agar Gel; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009841:Oligonucleotides
D009841:Oligonucleotides; D004262:DNA Restriction Enzymes
Immobilizovannye oligonukleotidy kak affinnye sorbenty dlia éndonukleaz restriktsii.