title: Naturally occurring serotype 2/subgroup II rotavirus reassortants in northern Brazil.
abstract: Nine serotype 2 human rotavirus strains were isolated in a community-based longitudinal study in Northern Brazil. Five of these strains had a 'long' RNA electrophoretic pattern and all five strains were determined to belong to subgroup II by ELISA assay, in contrast to properties common to serotype 2 human rotaviruses previously characterized. Hybridization studies of one of these unusual strains with 32P-labelled mRNAs derived from the prototype human strains Wa (serotype 1, subgroup II) and S2 (serotype 2, subgroup I) suggested that it was generated by a reassortment event in nature, in which a subgroup II, 'long' electropherotype rotavirus exchanged its serotype-specific gene and gene number 10 for the equivalent genes from a serotype 2, 'short' electropherotype virus.
authors.affiliation: Secao de Virologia do Instituto Evandro Chagas, FSESP, Para, Brasil.
authors.forename: J D
authors.initials: JD
authors.lastname: Mascarenhas
authors.forename: A C
authors.initials: AC
authors.lastname: Linhares
authors.forename: Y B
authors.initials: YB
authors.lastname: Gabbay
authors.forename: R B
authors.initials: RB
authors.lastname: de Freitas
authors.forename: E
authors.initials: E
authors.lastname: Mendez
authors.forename: S
authors.initials: S
authors.lastname: Lopez
authors.forename: C F
authors.initials: CF
authors.lastname: Arias
mesh_terms: D001938:Brazil; D006801:Humans; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012703:Serotyping
chemical_list: D012367:RNA, Viral