title: Expression of genes of ferritin subunits in human hepatoma cell lines.
abstract: The expression of ferritin genes has been studied in two well differentiated hepatoma cell lines, HepG2 and Hep3B, and in an undifferentiated cell line, HepPLC/PRF/5. The steady-state level of the H and L ferritin subunit mRNAs is different in the three cell lines, being most abundant in the HepG2 and Hep3B cells. The efficiency of promotion of transcription of the H and L gene promoters is not correlated with the levels of the two transcripts; moreover the half-life of the H and L mRNAs varies among the hepatoma cells. The different level of ferritin mRNAs in differentiated and undifferentiated cells appears to be mainly due to a different stability of the transcripts.
authors.affiliation: Dipartimento di Biochimica e Biotecnologie Mediche, II Facoltà di Medicina e Chirurgia, Napoli, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Costanzo
authors.forename: M A
authors.initials: MA
authors.lastname: Bevilacqua
authors.forename: M
authors.initials: M
authors.lastname: Giordano
authors.forename: F
authors.initials: F
authors.lastname: Cimino
mesh_terms: D015152:Blotting, Northern; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D002454:Cell Differentiation; D005293:Ferritins / Q000235:genetics*; D005786:Gene Expression Regulation; D006801:Humans; D066298:In Vitro Techniques; D008113:Liver Neoplasms; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
chemical_list: D012333:RNA, Messenger; D012334:RNA, Neoplasm; D005293:Ferritins