Inhibitor analysis of synapsis by resolvase.
Previously, we isolated several inhibitors that block the site-specific recombination reaction mediated by the Tn3-encoded resolvase protein. One class of inhibitors blocks resolvase binding to the recombination (res) sitc, and a second class inhibits synapse formation between resolvase and two directly repeated res sites. In this report, we identify an inhibitor, A20832, that does not inhibit resolvase binding to res, as measured by filter binding, or synapse formation. Inhibition of resolvase-promoted site-specific recombination by A20832 occurs postsynaptically at strand cleavage. DNase I analysis in the presence of A20832 indicates that only site I of res is bound by resolvase.
Flanagan
P M
PM
Department of Biological Sciences, University of Notre Dame, Indiana 46556.
Finn
M C
MC
Fennewald
M A
MA
D001665:Binding Sites; D004247:DNA / Q000378:metabolism; D004251:DNA Transposable Elements; D003850:Deoxyribonuclease I; D005229:Fatty Acids, Monounsaturated / Q000494:pharmacology*; D007700:Kinetics; D009713:Nucleotidyltransferases / Q000037:antagonists & inhibitors*; D010957:Plasmids; D011485:Protein Binding; D019895:Transposases
D004251:DNA Transposable Elements; D005229:Fatty Acids, Monounsaturated; C061161:A 20832; D004247:DNA; D009713:Nucleotidyltransferases; D019895:Transposases; D003850:Deoxyribonuclease I