Induction of complement factor B activity in human fibroblasts by IL-6/IFN-beta 2 and IFN-gamma.
Human skin fibroblasts synthesize and secrete complement Factor B, a component of the complement alternative pathway, when stimulated by mediators of inflammation such as lipopolysaccharide and various cytokines. Recombinant IL-6/IFN-beta 2 (E. coli) stimulates Factor B synthesis in fibroblasts but the effect is strongly potentiated by the addition of IFN-gamma. When both cytokines are added, the skin fibroblasts secrete significant amounts of biologically active Factor B detectable in a hemolysis test. This cooperative effect of IL-6, which is made by most tissue cells and monocytes and of IFN-gamma which is made by T-lymphocytes may play a role in local inflammatory processes. IL-6 and IFN-gamma also cooperate in the induction of (2'-5') A synthetase, a mediator of IFN action.
Barash
I
I
Molecular Genetics and Virology Department, Weizmann Institute of Science, Rehovot, Israel.
Fishelson
Z
Z
Revel
M
M
D015088:2',5'-Oligoadenylate Synthetase / Q000096:biosynthesis; D006528:Carcinoma, Hepatocellular / Q000378:metabolism; D002460:Cell Line; D011415:Complement Factor B / Q000096:biosynthesis* / Q000235:genetics; D004357:Drug Synergism; D004790:Enzyme Induction / Q000187:drug effects; D004792:Enzyme Precursors / Q000096:biosynthesis*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology*; D015850:Interleukin-6 / Q000494:pharmacology*; D008113:Liver Neoplasms / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
D004792:Enzyme Precursors; D007370:Interferon Type I; D015850:Interleukin-6; D011994:Recombinant Proteins; D007371:Interferon-gamma; D015088:2',5'-Oligoadenylate Synthetase; D011415:Complement Factor B