title: Rapid culture confirmation of adenovirus isolates using the adenoclone enzyme immunoassay (EIA) test kit.
abstract: The adenoclone enzyme immunoassay (EIA) test kit (Cambridge BioScience Corp., Worcester, MA) was evaluated using 43 adenovirus and 53 nonadenovirus (i.e., polio-, echo-, coxsackievirus types A,B, parainfluenza virus, and herpes simplex virus) isolates obtained during routine clinical diagnostic screening procedures. Forty-two of 43 confirmed adenovirus isolates were reactive upon testing by the adenoclone test kit. None of the 53 nonadenovirus isolates, including seven difficult-to-cultivate group A coxsackievirus isolates, cross-reacted with the EIA kit. The adenoclone EIA kit is an appropriate supplement to the regimen of clinical diagnostic procedures for the culture confirmation of adenovirus.
authors.affiliation: Department of Pathology and Laboratories, Nassau County Medical Center, East Meadow, New York 11554.
authors.forename: S M
authors.initials: SM
authors.lastname: Lipson
mesh_terms: D000256:Adenoviridae / Q000302:isolation & purification*; D000257:Adenoviridae Infections / Q000175:diagnosis; D005069:Evaluation Studies as Topic; D006801:Humans; D007124:Immunoenzyme Techniques