Interaction of non-piliated Neisseria gonorrhoeae strain 7122 and protein IA with an epithelial cell monolayer.
Studies with [14C] uracil-labelled bacteria revealed that the interaction of Neisseria gonorrhoeae with epithelial cells occurred in a time-dependent reaction which is slightly pH-dependent and optimal at pH 6.5. Immunofluorescence tests and immunoelectron microscopy of ultrathin sections confirmed the attachment of these bacteria to the epithelial cell membrane. The interaction of purified protein I with epithelial cells was time-dependent and reached equilibrium after four hours as shown by tracer experiments with 125I-labeled protein I. Cleavage experiments with trypsin followed by SDS-PAGE and autoradiography indicated that protein I (labeled with 125I) was associated with the membrane of the epithelial cells and only partly accessible by trypsin after its interaction with these mammalian cells. Immunofluorescence tests as well as immunoelectron microscopy with the monoclonal antibody G7A2C and gold-labeled protein A confirmed a dense association pattern of protein I with the cell monolayer.
Layh-Schmitt
G
G
Department of Pathobiology, University of Washington, Seattle 98195.
Schmitt
S
S
Buchanan
T M
TM
D000911:Antibodies, Monoclonal / Q000276:immunology; D001345:Autoradiography; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000382:microbiology / Q000648:ultrastructure; D004848:Epithelium / Q000382:microbiology* / Q000648:ultrastructure; D010861:Fimbriae, Bacterial / Q000378:metabolism* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008854:Microscopy, Electron / Q000379:methods; D009344:Neisseria gonorrhoeae / Q000378:metabolism* / Q000648:ultrastructure; D018272:Porins; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins; D018272:Porins; C057699:porin protein, Neisseria