Functional expression of a subtype of the Ca2+ channels in the embryonic rat hippocampus as detected by dual-fluorescence flow cytometric analysis.
We have analyzed the expression of the Ca2+ channels in hippocampal cell suspensions from the 18- to 20-day-old rat embryo using dual-fluorescence flow cytometry. A high concentration of K+ induced elevation of the cytoplasmic free Ca2+ concentration ([Ca2+]i) as well as membrane depolarization. The high K+-evoked [Ca2+]i increase was inhibited by phenytoin, but not by either nifedipine or nicardipine. These agents had no effect on the high K+-induced membrane depolarization. These findings suggest that a subtype corresponding to the low voltage-activated Ca2+ channel is expressed in the embryonic rat hippocampal cells.
Mitsumoto
Y
Y
Research Laboratory for Development of Medicine, School of Pharmacy, Hokuriku University, Ishikawa, Japan.
Mohri
T
T
D000818:Animals; D015220:Calcium Channels / Q000187:drug effects / Q000378:metabolism*; D004622:Embryo, Mammalian; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D010672:Phenytoin / Q000494:pharmacology; D051381:Rats
D015220:Calcium Channels; D005456:Fluorescent Dyes; D010672:Phenytoin