title: A comparison of norepinephrine- and ACTH-stimulated lipolysis in white and brown adipocytes of female rats.
abstract: 1. Isolated white and brown adipocytes (WFA and BFA) from the rat were compared with respect to their lipolytic responsiveness towards norepinephrine (NE) and adrenocorticotrophic hormone (ACTH). 2. NE yielded a Km value of 702.7 +/- 30.6 nM for WFA and 142.5 +/- 7.2 nM for BFA. The maximum lipolytic response (Vm) was 145.7 +/- 1.2 nmol glycerol/micrograms DNA/90 min for WFA and 23.7 +/- 0.2 nmol glycerol/micrograms DNA/90 min for BFA. 3. ACTH yield Km values of 31.6 +/- 1.5 and 31.9 +/- 3.1 nM for WFA and BFA, respectively. Vm values of 141.9 +/- 1.0 and 34.2 +/- 0.5 nmol glycerol/micrograms DNA/90 min were observed for WFA and BFA, respectively.
authors.affiliation: Department of Biochemistry, University of Port Elizabeth, Republic of South Africa.
authors.forename: M
authors.initials: M
authors.lastname: Van Heerden
authors.forename: W
authors.initials: W
authors.lastname: Oelofsen
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D002001:Adipose Tissue, Brown / Q000166:cytology / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000502:physiology*; D000818:Animals; D002021:Buffers; D002452:Cell Count; D005260:Female; D005990:Glycerol / Q000378:metabolism; D066298:In Vitro Techniques; D008066:Lipolysis / Q000502:physiology*; D009638:Norepinephrine / Q000502:physiology*; D051381:Rats; D013696:Temperature
chemical_list: D002021:Buffers; D000324:Adrenocorticotropic Hormone; D005990:Glycerol; D009638:Norepinephrine