A point mutation in the CYC1 UAS1 creates a new combination of regulatory elements that activate transcription synergistically.
Dissection of the upstream activation site 1 (UAS1) of the yeast CYC1 gene showed that the A and B regions respond individually to regulation by the HAP1 protein, and that a point mutation in the B region converts this region to a translation upstream factor (TUF)-regulated element. Combinatorial analyses revealed that the transacting factors involved with these wild-type and mutant UAS1 target sites combine to activate transcription in a synergistic manner. Furthermore, combinations of heterologous factors, made possible by the point mutation, create a new specificity of regulation that differs from regulation by any one factor individually.
Sousa
R
R
Départment de Biologie Moléculaire, UA 1149 CNRS, Institut Pasteur, Paris, France.
Arcangioli
B
B
D001483:Base Sequence; D001665:Binding Sites; D004271:DNA, Fungal / Q000235:genetics; D015696:Gene Products, tat; D005800:Genes, Fungal; D005809:Genes, Regulator; D009154:Mutation; D014176:Protein Biosynthesis; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic
D004271:DNA, Fungal; D015696:Gene Products, tat; D014157:Transcription Factors