title: Replication kinetics and cytopathic effect of hepatitis A virus.
abstract: The replication kinetics and c.p.e. of hepatitis A virus (HAV) strain HM-175 were shown to depend upon the passage level of the cell line, and the passage level and method of selection of the virus population. Maximum virus production under single-step growth curve conditions occurred as early as 24 to 28 h or as late as 10 days post-infection. Although rapid replication of an isolate of HM-715 (pHM-175) occurred initially in BS-C-1 cells, its most pronounced c.p.e. was induced in FRhK-4 cells. The replication kinetics of pHM-175 in BS-C-1 cells were similar to those in FRhK-4 cells, although a higher yield of virus was obtained in the latter. The HAV that generated c.p.e. in FRhK-4 cells was obtained by two different selection processes: virus passage, or cloning of large focus-forming variants from the radioimmunofocus assay. The c.p.e. and yield of infectious pHM-175 in FRhK-4 cells could be reduced by 3 mM-guanidine. Another HAV isolate, strain MD-1, isolated directly from contaminated ground water in cell culture demonstrated c.p.e. in FRhK-4 cells after passage as persistently infected A-549 cells.
authors.affiliation: University of North Carolina, School of Public Health, Chapel Hill 27514.
authors.forename: T
authors.initials: T
authors.lastname: Cromeans
authors.forename: H A
authors.initials: HA
authors.lastname: Fields
authors.forename: M D
authors.initials: MD
authors.lastname: Sobsey
mesh_terms: D000818:Animals; D002143:Callitrichinae; D002277:Carcinoma; D002460:Cell Line; D002522:Chlorocebus aethiops; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D019791:Guanidine; D006146:Guanidines / Q000494:pharmacology; D006507:Hepatovirus / Q000502:physiology*; D006801:Humans; D007668:Kidney; D007700:Kinetics; D008175:Lung Neoplasms; D008253:Macaca mulatta; D012692:Serial Passage; D014779:Virus Replication
chemical_list: D006146:Guanidines; D019791:Guanidine