Characterization of beta-adrenoceptors in the Syrian hamster Harderian gland: sexual differences and effects of either castration or superior cervical ganglionectomy.
Scatchard analysis of saturation isotherms of [125I]-iodopindolol was used to characterize beta-adrenoceptor density (Bmax) and affinity constant (Kd) in female and male hamster Harderian glands. Single-point experiments were also completed in intact females, intact males, and castrated or superior cervical ganglionectomized males. Scatchard analysis described a single population of binding sites with a Bmax of 292.2 +/- 45.1 fmol/mg protein (X +/- SEM, n = 6) in females and 18.2 +/- 3.0 fmol/mg protein (n = 6, P less than .001) in males. The affinity also varied significantly (P less than .05) with a Kd of 1.08 +/- 0.18 versus 0.26 + 0.05 nM (n = 6) in the Harderian gland of females and males, respectively. Single-point [125I]-IPIN (400 pM) binding values in females were 67.3 +/- 4.0, in intact males were 12.8 +/- 3.2, and in castrated males were 31.2 +/- 4.2 fmol/mg protein (n = 7-9). Superior cervical ganglionectomy induced no significant changes in receptor binding. The results indicate pronounced sexual differences in the density and affinity of beta-adrenoceptors in the hamster Harderian gland, which may be sex hormone dependent.
Pangerl
A
A
Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio 78284.
Pangerl
B
B
Buzzell
G R
GR
Jones
D J
DJ
Reiter
R J
RJ
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D006224:Cricetinae; D005260:Female; D005728:Ganglia, Sympathetic / Q000502:physiology*; D006243:Harderian Gland / Q000294:innervation / Q000378:metabolism*; D007700:Kinetics; D007765:Lacrimal Apparatus / Q000378:metabolism*; D008297:Male; D008647:Mesocricetus; D009919:Orchiectomy; D010052:Ovariectomy; D010869:Pindolol / Q000031:analogs & derivatives / Q000378:metabolism; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D012016:Reference Values; D012737:Sex Factors
D011943:Receptors, Adrenergic, beta; C029247:3-iodopindolol; D010869:Pindolol