Simulation of natural enamel caries in vitro with methylcellulose acid gels: effect of addition of calcium and phosphate ions.
A modified acid gel technique for producing caries-like lesions in dental enamel has been developed. The decalcifying medium consists of a 10% (w/v) methylcellulose gel acidified with 0.1 M lactic acid-sodium lactate at pH 4.5 and having a hydroxyapatite content of 0.05% (w/v). This medium can produce caries-like lesions in vitro at a slow rate, which are indistinguishable from natural enamel caries when examined by polarised light microscopy and contact microradiography.
Kotsanos
N
N
Department of Preventive Dentistry and Periodontics, Dental School, University of Thessaloniki, Greece.
Darling
A I
AI
Levers
B G
BG
Tyler
J E
JE
D000293:Adolescent; D001641:Bicuspid / Q000187:drug effects* / Q000473:pathology; D002326:Cariogenic Agents / Q000494:pharmacology*; D003731:Dental Caries / Q000473:pathology*; D003743:Dental Enamel / Q000187:drug effects* / Q000473:pathology; D017886:Durapatite; D005782:Gels; D006801:Humans; D006882:Hydroxyapatites / Q000494:pharmacology*; D066298:In Vitro Techniques; D008747:Methylcellulose / Q000494:pharmacology*
D002326:Cariogenic Agents; D005782:Gels; D006882:Hydroxyapatites; D008747:Methylcellulose; D017886:Durapatite