Intranasal immunization with proteoliposomes protects against influenza.
Worldwide, influenza virus remains a serious disease which has successfully eluded numerous attempts to design a consistently effective vaccine. In part, these attempts have been thwarted because of a lack of basic understanding of the mechanisms which mediate protection and recovery from influenza infection. A better understanding of the roles of secretory antibody, serum antibody and cell mediated immunity vis-à-vis protection and recovery from influenza infection has allowed us more rationally to approach the design and administration of a vaccine for influenza. We have constructed a vaccine composed of glycoproteins from the envelopes of either influenza of Sendai virus embedded in a lipid bilayer (immunosomes) mimicking the presentation of the virus to the cells during natural infection. Intranasal immunization with these immunosomes induces an adequate systemic Ir compared with intramuscular immunization and a superior local IgA response. These animals were specifically protected from virus challenge.
el Guink
N
N
Department of Microbiology and Immunology, Albany Medical College, NY 12208.
Kris
R M
RM
Goodman-Snitkoff
G
G
Small
P A
PA
Mannino
R J
RJ
D000281:Administration, Intranasal; D000818:Animals; D002642:Chick Embryo; D007114:Immunization; D007252:Influenza Vaccines / Q000008:administration & dosage*; D008171:Lung Diseases / Q000276:immunology; D051379:Mice; D009668:Nose Diseases / Q000276:immunology; D009976:Orthomyxoviridae Infections / Q000276:immunology*; D010222:Parainfluenza Virus 1, Human; D018184:Paramyxoviridae Infections / Q000276:immunology; D011510:Proteolipids / Q000008:administration & dosage / Q000032:analysis / Q000276:immunology*
D007252:Influenza Vaccines; D011510:Proteolipids; C020485:proteoliposomes