[The use of commercial immunoenzyme and latex preparations for the demonstration of human rotavirus antigens].
The results of using enzyme immunoassay and latex preparations for the diagnosis of rotavirus gastroenteritis are presented. High effectiveness of the enzyme immunoassay system developed in the USSR with latex diagnostic agents, such as Rotalex (Orion Diagnostica, Finland), Slidex Rota Kit (BioMérieux, France), The Wellcome Rotavirus Latex Kit (Wellcome Foundation Ltd., Great Britain), 48-63% and 21-41% respectively, has been noted. The results of the comparison of the system developed in the USSR with Wellcozyme Rotavirus, an enzyme immunoassay system manufactured by Wellcome Foundation Ltd. (Great Britain), are practically comparable. The results of the block test and the confirmation test used for control indicate that the Soviet preparation is specific. Materials on the practical evaluation of the assay system at health institutions are presented. Good prospects for the use of this system in the diagnosis of rotavirus gastroenteritis, as well as in the realization of epidemiological surveillance on this infection, are substantiated.
Gorbachev
E N
EN
Verbov
V N
VN
Artiukhov
A I
AI
Noskov
F S
FS
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002417:Cattle; D005069:Evaluation Studies as Topic; D005243:Feces / Q000382:microbiology; D005759:Gastroenteritis / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation*; D007841:Latex Fixation Tests / Q000295:instrumentation*; D011933:Reagent Kits, Diagnostic; D012401:Rotavirus / Q000276:immunology* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000175:diagnosis / Q000382:microbiology; D013552:Swine / Q000382:microbiology
D000956:Antigens, Viral; D011933:Reagent Kits, Diagnostic
Ispol'zovanie kommercheskikh immunofermentnykh i lateksnykh preparatov dlia indikatsii antigenov rotaviusa cheloveka.