VIP modulation of cultured glial cells of the rat retina.
Cultured retinal glial cells from the rat are responsive to modulation by vasoactive intestinal peptide (VIP). VIP (1 X 10(-6) M) elevated the intracellular cyclic AMP concentration from the basal level of (4.4-11.1) p mole/mg protein to (354-440) p mole/mg protein in three minutes at 25 degrees C. The half-maximal concentration is 4.8 X 10(-8) M, which is similar to that observed in the cultured retinal glial cells from the chick embryo.
Koh
S W
SW
Department of Ophthalmology, University of Maryland, Baltimore 21201.
Roberge
F G
FG
D000818:Animals; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D009457:Neuroglia / Q000032:analysis* / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew; D011964:Receptors, Gastrointestinal Hormone / Q000032:analysis* / Q000378:metabolism; D018005:Receptors, Vasoactive Intestinal Peptide; D012160:Retina / Q000032:analysis* / Q000166:cytology / Q000378:metabolism; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*
D011964:Receptors, Gastrointestinal Hormone; D018005:Receptors, Vasoactive Intestinal Peptide; D014660:Vasoactive Intestinal Peptide; D000242:Cyclic AMP