A high-performance liquid chromatographic method for the determination of polyphosphoinositides in brain.
A simple high-performance liquid chromatographic method for the determination of endogenous phosphatidylinositol 4-phosphate (PIP) and phosphatidylinositol 4,5-bisphosphate (PIP2) in brain has been developed. PIP and PIP2 were derivatized with 9-anthryldiazomethane to yield (9-anthryl)PIP and di(9-anthryl)-PIP2. The derivatives were separated on a reversed-phase column using isocratic elution and detected with a uv detector. The detection limits of PIP and PIP2 were 0.25 micrograms. The method with uv detection was sufficiently sensitive to measure the concentrations of PIP and PIP2 in rat brain. The levels of PIP and PIP2 were increased in developing rat brain and were decreased after 10 min of ischemia.
Nakamura
T
T
Tsukuba Research Laboratories, Eisai Company, Ltd., Ibaraki, Japan.
Hatori
Y
Y
Yamada
K
K
Ikeda
M
M
Yuzuriha
T
T
D000818:Animals; D000873:Anthracenes; D001921:Brain / Q000254:growth & development; D001923:Brain Chemistry; D002545:Brain Ischemia / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D008297:Male; D013058:Mass Spectrometry; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D000873:Anthracenes; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols; C027347:9-diazomethylanthracene