title: Identification of B2 bradykinin binding sites in the guinea pig nasal turbinate.
abstract: Specific high affinity BK binding sites in the nasal turbinate of the guinea pig have been demonstrated. Specific [3H]BK binding (10-330 pM) was saturable, and nonlinear least squares analysis indicated the presence of a high affinity binding site with a Kd value of 60 (50-78) pM and a Bmax value of 13.1 = 2.0 fmol/mg protein. In inhibition experiments, D-Phe7-BK (a B2 antagonist) inhibited [3H]BK binding with a Ki value of 23 nM, while des-Arg9[Leu8]-BK (a B1 antagonist) had no effect up to a concentration of 10 microM. These studies indicate the presence of B2 BK receptors in the guinea pig nasal turbinate.
authors.affiliation: Department of Pharmacology, University of Arizona Health Sciences Center, Tucson 85724.
authors.forename: Y
authors.initials: Y
authors.lastname: Fujiwara
authors.forename: J W
authors.initials: JW
authors.lastname: Bloom
authors.forename: C R
authors.initials: CR
authors.lastname: Mantione
authors.forename: H I
authors.initials: HI
authors.lastname: Yamamura
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D001920:Bradykinin / Q000378:metabolism*; D006168:Guinea Pigs; D007700:Kinetics; D008297:Male; D018002:Receptors, Bradykinin; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D014420:Turbinates / Q000378:metabolism*
chemical_list: D018002:Receptors, Bradykinin; D017981:Receptors, Neurotransmitter; D001920:Bradykinin