title: Bacterial overgrowth in the jejunum of ICR mice and Wistar rats orally administered with a single lethal dose of fusarenon-X, a trichothecene mycotoxin.
abstract: A single oral dose of fusarenon-X (F-X), a trichothecene mycotoxin, resulted in abnormal microflora in the jejunum in ICR mice and Wistar rats with some differences in dose response between the species. In the acute phase, enterobacteria, streptococci, Clostridium perfringens and bacteroides showed remarkably increased counts in the jejunum of mice and rats dosed with F-X while lactobacilli showed a decrease in count. F-X brought an invasion of Pseudomonas aeruginosa into the livers, lungs, kidneys and spleens of ICR mice. Changes in the jejunal microflora appeared after 7 h in ICR mice and after 24 h in Wistar rats after a single oral dose of F-X of 7.5 and 4.0 mg/kg b.w., respectively, and the microflora returned to its normal state at 72 h in mice and 96 h in rats. The changes of intestinal microflora were followed by alterations in the growth curves of both animal species. The pH in the glandular stomach was also greatly enhanced before changes in the jejunal microflora. Acute F-X intoxication may be an involved manifestation of essential cytotoxicity of F-X mycotoxin alone and secondary bacterial overgrowth in the bowel.
authors.affiliation: National Institute of Health, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Morishita
authors.forename: K
authors.initials: K
authors.lastname: Nagasawa
authors.forename: N
authors.initials: N
authors.lastname: Nakano
authors.forename: K
authors.initials: K
authors.lastname: Shiromizu
mesh_terms: D000818:Animals; D003016:Clostridium perfringens / Q000254:growth & development* / Q000302:isolation & purification; D015169:Colony Count, Microbial; D004926:Escherichia coli / Q000254:growth & development* / Q000302:isolation & purification; D007583:Jejunum / Q000187:drug effects / Q000382:microbiology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011550:Pseudomonas aeruginosa / Q000254:growth & development* / Q000302:isolation & purification; D051381:Rats; D011919:Rats, Inbred Strains; D012717:Sesquiterpenes / Q000633:toxicity*; D014255:Trichothecenes / Q000633:toxicity*
chemical_list: D012717:Sesquiterpenes; D014255:Trichothecenes; C002469:fusarenon-X