title: Resistance to the Bacillus sphaericus toxin in cultured mosquito cells.
abstract: Increasing doses of Bacillus sphaericus toxin were used to select a toxin-resistant cell line from the Culex quinquefasciatus line. This resistant cell line was proven to be C. quinquefasciatus in origin by isozyme analysis and karyotype. The resistant line bound fluorescent-labeled toxin as did the unselected susceptible line. A high level of resistance was quickly achieved, and this level was maintained after 4 mo. culture in the absence of toxin.
authors.affiliation: Department of Zoology, Arizona State University, Tempe 85287.
authors.forename: J M
authors.initials: JM
authors.lastname: Schroeder
authors.forename: C
authors.initials: C
authors.lastname: Chamberlain
authors.forename: E W
authors.initials: EW
authors.lastname: Davidson
mesh_terms: D000818:Animals; D001407:Bacillus; D001427:Bacterial Toxins / Q000633:toxicity*; D002460:Cell Line; D002478:Cells, Cultured; D009033:Culicidae / Q000166:cytology*; D004352:Drug Resistance, Microbial
chemical_list: D001427:Bacterial Toxins; C046134:binB protein, Bacillus sphaericus