title: Differences in the release of 5'-nucleotidase and alkaline phosphatase from plasma membrane of several cell types by PI-PLC.
abstract: 1. We have compared the effect of phosphatidyl inositol specific phospholipase C (PI-PLC) on the attachment of both 5'-nucleotidase and alkaline phosphatase to the liver plasma membrane from different species. 2. Our results demonstrate differences in the susceptibilities of both enzymes to PI-PLC treatment in relation to their origin. 3. These results were confirmed by immunoblotting using polyclonal anti-5'-nucleotidase antibodies. 4. In addition, in a single animal, susceptibility of both enzymes to PI-PLC treatment is different from one tissue to another. 5. The different percentages of released enzymes could be explained either by a polymorphism in the anchoring of these proteins at the cell surface membrane, or by a different steric hindrance or environment at the cleavage site itself.
authors.affiliation: Université de Nantes, C.H.R.U-Hôtel Dieu, Laboratoire de Biochimie Médicale, France.
authors.forename: M
authors.initials: M
authors.lastname: Zekri
authors.forename: J
authors.initials: J
authors.lastname: Harb
authors.forename: S
authors.initials: S
authors.lastname: Bernard
authors.forename: G
authors.initials: G
authors.lastname: Poirier
authors.forename: C
authors.initials: C
authors.lastname: Devaux
authors.forename: K
authors.initials: K
authors.lastname: Meflah
mesh_terms: D015720:5'-Nucleotidase; D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism*; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting; D008099:Liver / Q000166:cytology / Q000201:enzymology; D051379:Mice; D009708:Nucleotidases / Q000378:metabolism*; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D054801:Phosphoinositide Phospholipase C; D010727:Phosphoric Diester Hydrolases; D051381:Rats; D013045:Species Specificity
chemical_list: D009708:Nucleotidases; D000469:Alkaline Phosphatase; D015720:5'-Nucleotidase; D010727:Phosphoric Diester Hydrolases; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase