[Inhibition of release of prostaglandins and leukotrienes from calcimycin-induced mouse peritoneal macrophages and bovine aorta endothelial cells by anisodamine].
[3H]Arachidonic acid (AA)-prelabeled mouse peritoneal macrophages were stimulated by calcium ionophore A-23187 to release [3H]AA metabolites. The major labeled products which were co-chromatographed with the authentic PG and LT standards by TLC and determined by liquid scintillation were 6-keto-PGF1 alpha, PGE2, LTC4, and LTB4. Anisodamine (Ani) significantly inhibited the A-23187-induced release of PG and LT from mouse macrophages in a dose-dependent manner. In the presence of Ani at 0.5 mmol/L, the A-23187-induced release of 6-keto-PGF1 alpha, PGE2, LTC4 and LTB4 was reduced by 57%, 20%, 53% and 49%, respectively. A-23187-induced release of 6-keto-PGF1 alpha measured by RIA from bovine aorta endothelial cells was also significantly inhibited by Ani in a dose-dependent manner. These results indicate that the calcium-antagonistic effects of Ani may not only play a significant role in its inhibiting release of PG and LT, but also contribute to its salutary effects in the treatment of septic shock.
Li
J
J
Jiang
Y Y
YY
Yue
T L
TL
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D000818:Animals; D001011:Aorta / Q000166:cytology; D000001:Calcimycin / Q000494:pharmacology; D002417:Cattle; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000378:metabolism*; D005260:Female; D007975:Leukotriene B4 / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D051379:Mice; D010529:Peritoneal Cavity / Q000166:cytology; D011453:Prostaglandins / Q000378:metabolism*; D011458:Prostaglandins E / Q000378:metabolism; D013189:SRS-A / Q000378:metabolism; D012991:Solanaceous Alkaloids / Q000494:pharmacology*
D011453:Prostaglandins; D011458:Prostaglandins E; D013189:SRS-A; D012991:Solanaceous Alkaloids; C003922:anisodamine; D007975:Leukotriene B4; D000001:Calcimycin; D015121:6-Ketoprostaglandin F1 alpha