Effect of lipoxygenase products on choriogonadotropin secretion by cultured human choriocarcinoma JEG-3 cells pre- and post-stimulation with epidermal growth factor and a phorbol ester.
Previous studies using arachidonic acid and preferential inhibitors of the arachidonic acid pathway have implicated the lipoxygenase system in choriogonadotropin (hCG) secretion by JEG-3 cells. Presently, JEG-3 cells are used in order to examine the effect of lipoxygenase products on hCG secretion. Results show that 30 microM 15-hydroxyeicosatetraenoic acid (15-HETE) induces an approximately 3-fold increase in basal hCG secretion, while 5-HETE, 12-HETE, and leukotriene LTA4 have no significant effect. In addition, 15-HETE potentiates the stimulation of hCG secretion induced by 3 nM epidermal growth factor (EGF), but has no significant effect on the stimulation of hCG induced by 22 nM tetradecanoylphorbol acetate (TPA). The present study further implicates the arachidonic acid pathway in the control of hCG secretion and documents that the effect of EGF can be rate-limited by a product of the lipoxygenase system.
Ilekis
J
J
Department of Obstetrics and Gynecology, Michael Reese Hospital and Medical Center, Chicago, IL 60616.
Benveniste
R
R
D002460:Cell Line; D002822:Choriocarcinoma / Q000378:metabolism / Q000473:pathology*; D006063:Chorionic Gonadotropin / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005260:Female; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000494:pharmacology*; D017572:Leukotriene A4; D015289:Leukotrienes / Q000494:pharmacology*; D008084:Lipoxygenase / Q000378:metabolism*; D010703:Phorbol Esters / Q000494:pharmacology*; D011247:Pregnancy; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014594:Uterine Neoplasms / Q000378:metabolism / Q000473:pathology*
D006063:Chorionic Gonadotropin; D006893:Hydroxyeicosatetraenoic Acids; D017572:Leukotriene A4; D015289:Leukotrienes; D010703:Phorbol Esters; D004815:Epidermal Growth Factor; D008084:Lipoxygenase; D013755:Tetradecanoylphorbol Acetate