Evidence for the in vivo production of interleukin-3 in lethally irradiated mice transplanted with syngeneic murine splenocytes.
We wish to demonstrate new evidence for the in vivo production of interleukin-3 (IL-3). Syngeneic murine splenocytes were transplanted into lethally irradiated mice. The spleen cells in these transplant mice spontaneously produced IL-3 in cultures, and IL-3-like activity was detected in the serum. Chronological changes of the Thy-1 antigen expression on the bone marrow cells, and the number of myeloid progenitors in the bone marrow from post-transplant mice correlated with the amount of IL-3 produced in vivo. These results suggest that IL-3 may be produced by activated T cells during the syngeneic mixed lymphocyte reaction induced in vivo.
Hirokawa
M
M
Third Department of Internal Medicine, Akita University School of Medicine, Japan.
Takatsu
H
H
Chubachi
A
A
Kudo
K
K
Takahashi
T
T
Yoshida
K
K
Miura
A B
AB
D000818:Animals; D000954:Antigens, Surface; D001853:Bone Marrow; D002474:Cell-Free System; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000032:analysis; D005260:Female; D006412:Hematopoietic Stem Cells / Q000145:classification; D007377:Interleukin-3 / Q000096:biosynthesis* / Q000097:blood; D017710:Lymphocyte Transfusion; D008214:Lymphocytes / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010641:Phenotype; D011184:Postoperative Period; D011828:Radiation Chimera; D013154:Spleen / Q000378:metabolism / Q000637:transplantation; D018800:Thy-1 Antigens; D014185:Transplantation, Isogeneic
D000954:Antigens, Surface; D003115:Colony-Stimulating Factors; D007377:Interleukin-3; D018800:Thy-1 Antigens