Human topoisomerase 1 messenger RNA is not destabilized by the herpes simplex virus type 2 virion-associated shut-off function.
A cDNA for human topoisomerase I (Topo 1) was used to identify a 4.1 kb polyadenylated Topo 1 mRNA in methotrexate-resistant human KB cells that are permissive for herpes simplex virus type 2 (HSV-2) infection. Using these cells, no effect of the HSV-2-associated early shut-off function on levels of Topo-1 mRNA was observed up to 6 hours postinfection, whereas the actin mRNA level was 22% cellular transcripts are susceptible. The level of several host-cell polyadenylated RNAs detected as cDNA clones (class 3 transcripts) were unchanged 8 hours after HSV-2 infection, and other cellular transcripts (class 2) actually accumulated at postinfection.
Bastow
K F
KF
Department of Pharmacology, School of Medicine, University of North Carolina, Chapel Hill 27599-7365.
Zhou
B S
BS
Cheng
Y C
YC
D002472:Cell Transformation, Viral; D004264:DNA Topoisomerases, Type I / Q000235:genetics*; D005796:Genes; D006801:Humans; D007624:KB Cells; D008727:Methotrexate / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D018139:Simplexvirus / Q000235:genetics*; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics; D014158:Transcription, Genetic; D014771:Virion / Q000235:genetics*
D012333:RNA, Messenger; D013762:Tetrahydrofolate Dehydrogenase; D004264:DNA Topoisomerases, Type I; D008727:Methotrexate