title: Increased diuron resistance in the joint expression of mutations located at the DIU2, DIU3 and DIU4 loci of Saccharomyces cerevisiae.
abstract: In Saccharomyces cerevisiae, diuron blocks the respiratory pathway at the level of the bc1 complex. Two mitochondrially inherited loci, DIU1 and DIU2, located in the cytochrome b gene, and two nuclearly inherited loci, DIU3 and DIU4, have previously been identified. The present work genetically characterizes two double mutants. One mutant, Diu-217, carries two nuclearly inherited mutations, diu3-217a and diu-217b; the second mutant, Diu-783, carries the previously described nuclear mutation diu3-783 and a mitochondrial mutation diu2-783. Each mutation, independent of its location, exhibits a weak diuron resistance. The joint expression of two or three mutations leads to a cumulative or a cooperative enhanced diuron-resistant phenotype.
authors.affiliation: Université Catholique de Louvain, Laboratoire de Génétique Microbienne, Belgium.
authors.forename: B
authors.initials: B
authors.lastname: Meunier
authors.forename: A M
authors.initials: AM
authors.lastname: Colson
mesh_terms: D000483:Alleles; D003573:Cytochrome b Group / Q000235:genetics; D004237:Diuron / Q000494:pharmacology*; D004352:Drug Resistance, Microbial / Q000235:genetics*; D014450:Electron Transport Complex III / Q000235:genetics; D005091:Exons; D005796:Genes; D005800:Genes, Fungal; D008928:Mitochondria / Q000378:metabolism; D009154:Mutation; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism
chemical_list: D003573:Cytochrome b Group; D004237:Diuron; D014450:Electron Transport Complex III