Epstein-Barr virus (EBV). VII. Established lymphoid cell line (IVPat-88) obtained from synovial fluid of a patient with aseptic arthritis.
Attempts have been made to culture mononuclear cells from synovial fluid of 8 patients with arthropathy, and have led to the development of the lymphoid cell line IVPat-88. Cell line has been propagated by serial passages for more than 14 weeks in continuous culture. The cells grew as single, free-floating individuals, or in dense clumps without adherence to glass or plastic surface. All these cells were identified as altered lymphoblasts because of their growth pattern and uniform morphology, and the presence of Epstein-Barr Viral Capsid Antigen (VCA) in 5 to 10% of the cells. The cell concentration varied during the period of culture from about 300,000 to 1,700,000 cells per ml, and mean doubling time during phases of active growth was 42 and 60 hours in MEM and RPMI 1640 tissue culture media, respectively. The methods used and the characteristics of the cell line are described.
Pătraşcu
I V
IV
Ghenoiu
O
O
Tache
M
M
Stoicescu
M
M
Cajal
N
N
D000914:Antibodies, Viral / Q000032:analysis*; D000921:Antibody-Producing Cells / Q000276:immunology; D001168:Arthritis / Q000276:immunology*; D002213:Capsid / Q000276:immunology; D002460:Cell Line; D002469:Cell Separation; D002478:Cells, Cultured; D005260:Female; D005455:Fluorescent Antibody Technique; D006566:Herpesviridae Infections / Q000276:immunology; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D008297:Male; D009000:Monocytes / Q000276:immunology*; D013582:Synovial Fluid / Q000276:immunology*
D000914:Antibodies, Viral