Endothelin stimulates DNA synthesis in Swiss 3T3 cells. Synergy with polypeptide growth factors.
The vasoactive peptide endothelin is shown to be a potent mitogen for Swiss 3T3 cells. Although endothelin has little effect on DNA synthesis when added alone to cells in serum-free medium, the peptide synergizes very strongly with several other growth factors. A half-maximal response to endothelin is observed at approx. 0.3 nM, with a maximal effect at 3 nM. Over the same concentration range, endothelin stimulates a 2-fold increase in the accumulation of cellular inositol phosphates. Endothelin may prove to be a useful additional agonist for studying the signalling pathways involved in the control of 3T3-cell proliferation.
Brown
K D
KD
Department of Biochemistry, AFRC Institute of Animal Physiology, Cambridge, U.K.
Littlewood
C J
CJ
D000818:Animals; D001667:Binding, Competitive; D001839:Bombesin / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects*; D004247:DNA / Q000096:biosynthesis*; D004357:Drug Synergism; D016232:Endothelins; D004815:Epidermal Growth Factor / Q000494:pharmacology; D066246:ErbB Receptors / Q000378:metabolism; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology*; D007295:Inositol Phosphates / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology; D051379:Mice; D010455:Peptides / Q000008:administration & dosage* / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D015291:Transforming Growth Factors / Q000494:pharmacology
D016232:Endothelins; D006133:Growth Substances; D007295:Inositol Phosphates; D007328:Insulin; D010455:Peptides; D010716:Phosphatidylinositols; D010982:Platelet-Derived Growth Factor; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor; D015291:Transforming Growth Factors; D004247:DNA; D066246:ErbB Receptors; D001839:Bombesin