Characteristics of Epstein-Barr virus-carrying lymphoblastoid cells derived from human tonsil: EBV production and autoantibody response.
Tonsillar and peripheral blood lymphocytes from 20 patients with tonsillitis were examined for production of Epstein-Barr virus (EBV) and antinuclear antibodies within 2 weeks after EBV transformation. All established lymphoblastoid cell lines (LCLs) contained more than 90% EBV nuclear antigen-positive cells. However, no LCLs produced biologically active virus. On the other hand, 10 (50%) out of 20 tonsillar LCLs and 4 (20%) out of 20 peripheral blood LCLs produced autoantibodies. These results showed that there are differences in an EBV-induced lymphocyte autoantibody response between tonsillar lymphocytes and peripheral blood lymphocytes.
Takimoto
T
T
Department of Otolaryngology, School of Medicine, Kanazawa University, Japan.
Tanaka
S
S
Ishikawa
S
S
Masuda
K
K
Umeda
R
R
D000974:Antibodies, Antinuclear; D001323:Autoantibodies; D002472:Cell Transformation, Viral; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D008214:Lymphocytes; D014066:Palatine Tonsil / Q000166:cytology*; D014069:Tonsillitis / Q000097:blood
D000974:Antibodies, Antinuclear; D001323:Autoantibodies