[Flow cytometric analysis of the effect of activated macrophages on alveolar cell carcinoma of lung in vitro].
Evidences have shown that activated macrophages were cytotoxic to tumor cells but the mechanism of this killing effect is not well understood. Flow cytometry was used to study the effect of activated macrophages on cell proliferation cycle of alveolar cell carcinoma of lung (A 549). Among 74 C57BL/6J mice, 30 as experimental group were given corynebacterium parvum 0.5 ml (1 mg)/mouse intraperitoneally, and 44 as control group were given normal saline 0.5 ml/mouse instead. Peritoneal macrophages of both groups were co-cultured with A 549 in the ratio of 10:1 and 20:1 for 24 and 48 hrs respectively. Specimens were assayed with LXJ-Laser flow cytometer and relative % of tumor cells in G1/O, S and G2 + M phased were calculated by morphometry using Baisech's mathematical model and Zeiss OPTON Video plan. Results indicated that in the experimental group with E/T 20:1, co-cultured for 24 hrs., the % of tumor cells in S phase was markedly decreased (28.44%) and those in G1/0 phase was increased (60.18%) (P less than 0.01); while those of the control group were 40.41% and 49.99% respectively. Changes present in S and G1/0 phase tumor cells of the experimental group indicate that activated macrophages may block tumor cells from the G1/0 to S phase and thus affect their proliferation.
Wang
B M
BM
D002282:Adenocarcinoma, Bronchiolo-Alveolar / Q000473:pathology*; D000818:Animals; D002455:Cell Division; D005434:Flow Cytometry; D008175:Lung Neoplasms / Q000473:pathology*; D008262:Macrophage Activation; D008264:Macrophages / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL