A thiophilic adsorbent for the one-step high-performance liquid chromatography purification of monoclonal antibodies.
Thiophilic adsorption chromatography, developed originally by Porath and colleagues (1985, FEBS Lett. 185, 306-310) for conventional chromatographic techniques, was transformed to the HPLC mode upon preparation of new and improved sulfur-containing silica beads. The new thiophilic adsorbent is of high capacity and is suitable for the rapid and single-step purification of all subclasses of monoclonal and polyclonal antibodies. Due to its broad specificity, the thiophilic silica column is an efficient, stable, and inexpensive substitute for protein A and protein G columns used today to purify antibodies.
Nopper
B
B
Department of Biophysics, Weizmann Institute of Science, Rehovot, Israel.
Kohen
F
F
Wilchek
M
M
D000327:Adsorption; D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D015200:Fluoroimmunoassay; D007074:Immunoglobulin G / Q000302:isolation & purification; D008297:Male; D051379:Mice; D051381:Rats; D012822:Silicon Dioxide; D013438:Sulfhydryl Compounds
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D013438:Sulfhydryl Compounds; D012822:Silicon Dioxide