title: Effect of ammonium chloride on the astrocyte benzodiazepine receptor.
abstract: We have carried out studies on the effect of ammonium chloride on the astrocyte benzodiazepine receptor. Scatchard analysis of the binding of [3H]Ro-5-4864 to homogenates prepared from primary astrocyte cultures showed a significant decrease in Kd (27% with 2 mM NH4Cl; 32% with 5 mM NH4Cl; 25% with 10 mM NH4Cl) and Bmax (14% with 10 mM NH4Cl). These findings indicate that ammonium chloride can affect the astrocyte benzodiazepine receptor, and that such receptor changes may contribute to ammonia-induced encephalopathy.
authors.affiliation: Department of Pathology, University of Miami/Jackson Memorial Hospital, FL.
authors.forename: I
authors.initials: I
authors.lastname: Ducis
authors.forename: L O
authors.initials: LO
authors.lastname: Norenberg
authors.forename: M D
authors.initials: MD
authors.lastname: Norenberg
mesh_terms: D000643:Ammonium Chloride / Q000494:pharmacology*; D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D001570:Benzodiazepinones / Q000378:metabolism; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D003292:Convulsants / Q000494:pharmacology; D007700:Kinetics; D051381:Rats; D011916:Rats, Inbred F344; D011963:Receptors, GABA-A / Q000187:drug effects / Q000378:metabolism*
chemical_list: D001570:Benzodiazepinones; D003292:Convulsants; D011963:Receptors, GABA-A; D000643:Ammonium Chloride; C028513:4'-chlorodiazepam