[Experimental embryologic, biochemical and molecular biology approaches to the identification of embryonic inducers].
Problems of the mechanisms of embryonic induction in vertebrate development have been considered on the basis of author's experimental data. Though several polypeptide factors with certain inducing activity have been identified recently, molecular genetic mechanisms of their effect on embryonic target cells remains largely unclear. One of possible causes of very slow progress in this area of developmental biology is an inadequate system of biotesting of inducers at tissue level (ectoderm of early amphibian gastrulae) using histological criteria. A necessity for carrying out similar studies on cellular level and estimating effect of inducers using immunochemical and molecular biological methods has been postulated. Methods allowing to carry out biotesting of inducers on cell suspension or aggregate of a one type of embryonic cells have been proposed. New approaches, combining the methods of experimental embryology and molecular biology, to studies of embryonic inducers, receptors, and their mRNA, have been analyzed.
Mikhaĭlov
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D000663:Amphibians / Q000196:embryology; D000818:Animals; D001681:Biological Assay / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects*; D004626:Embryology / Q000379:methods; D004627:Embryonic Induction / Q000187:drug effects*; D006133:Growth Substances / Q000032:analysis* / Q000494:pharmacology; D009024:Morphogenesis / Q000187:drug effects; D010455:Peptides / Q000032:analysis* / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000187:drug effects
D006133:Growth Substances; D010455:Peptides; D011956:Receptors, Cell Surface
Eksperimental'no-émbriologicheskie, biokhimicheskie i molekuliarno-biologicheskie podkhody k identifikatsii émbrional'nykh induktorov.