title: [Ca2+-protease--an enzyme of the metabolism of cytoskeletal proteins in the olfactory membrane of vertebrates].
abstract: Two forms of Ca(++)-activated protease (calpain I and calpain II) associated with an endogenous inhibitor (calpastatin) were detected in a cytosolic fraction of the olfactory tissue of vertebrates (pig, rat). Using ion exchange chromatography on DEAE-cellulose column, calpain I is divided into 2 peaks (eluting by 0.07-0.15 and 0.22-0.25 M NaCl), and calpain II is eluted by 0.35-0.40 M NaCl. The calpain activity was detected in fractions eluted by 0.1-0.17 M NaCl. The Ca(++)-activated protease was demonstrated also in a fraction of cytoskeleton of olfactory tissue insoluble in a 1% solution of Triton X-100. The activity can be detected by Ca(++)-dependent destruction of exogenous substrate (casein), and by Ca(++)-dependent degradation of cytoskeletal endogenous proteins (16, 18 and 20 kDa), of which one may be calmodulin.
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Chistiakova
authors.forename: E V
authors.initials: EV
authors.lastname: Parfenova
mesh_terms: D000818:Animals; D002154:Calpain / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D002848:Chromatography, DEAE-Cellulose / Q000379:methods; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D003599:Cytoskeleton / Q000032:analysis / Q000201:enzymology; D003600:Cytosol / Q000032:analysis / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D009831:Olfactory Mucosa / Q000032:analysis / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity / Q000502:physiology; D013552:Swine
chemical_list: D003598:Cytoskeletal Proteins; D002154:Calpain
vernacular_title: Ca2+-proteaza--ferment metabolizma belkov tsitoskeleta oboniatel'noĭ vystilki pozvonochnykh.