[An immunoenzyme method of isolation of foot-and-mouth disease virus by using beta-lactamase conjugate with virus-specific antibodies].
It was shown that in was feasible to use conjugates of virus-specific antibodies and beta-lactamase from Bacillus licheniformis 749/c to identify aphthosa virus antigens. The antigen titers determined by enzyme immunoassay (EIA) using a beta-lactamase conjugate were 5-64 times higher than the analogous indices of the complement fixation test. Unlike EIA, that by using the antibody conjugates with peroxidase or alkaline phosphatase there were observed no "background" responses.
Mishchenko
V A
VA
Bazarov
M A
MA
Koniushkina
T B
TB
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D000918:Antibody Specificity; D005537:Aphthovirus / Q000276:immunology / Q000302:isolation & purification*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000382:microbiology*; D005536:Foot-and-Mouth Disease / Q000175:diagnosis / Q000382:microbiology*; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000382:microbiology; D001618:beta-Lactamases
D000914:Antibodies, Viral; D001618:beta-Lactamases
Immunofermentnyĭ metod opredeleniia virusa iashchura s ispo'lzovaniem ko''niugata beta-laktamazy s virisspetsifi- cheskimi antitelami.