Establishment of a cell line producing bone morphogenetic protein from a human osteosarcoma.
A human osteosarcoma cell line was established from a biopsy specimen from a 13-year-old girl. The osteosarcoma tissue was maintained in athymic nude mice (Balb C nu/nu) by serial transplantation for three years. The tumor was excised from a host mouse and digested with collagenase. The isolated cells were cultured by 98 passages in 14 months, and clones of osteosarcoma cells were obtained by limiting dilution. A clone named human osteosarcoma cell 6 (H-OS-6) that showed the osteoblastic phenotypes of productions of bone morphogenetic protein (BMP) and alkaline phosphatase and a response to human parathyroid hormone (h-PTH 1-34) was selected. The morphology of its chromosomes indicated its human origin. This human osteosarcoma cell line is unique in producing BMP under in vitro conditions.
Takaoka
K
K
Department of Orthopaedic Surgery, Osaka University Medical School, Japan.
Yoshikawa
H
H
Masuhara
K
K
Sugamoto
K
K
Tsuda
T
T
Aoki
Y
Y
Ono
K
K
Sakamoto
Y
Y
D000293:Adolescent; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D019485:Bone Morphogenetic Proteins; D002999:Clone Cells / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D005260:Female; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D009368:Neoplasm Transplantation; D012516:Osteosarcoma / Q000378:metabolism*; D010281:Parathyroid Hormone / Q000494:pharmacology; D014176:Protein Biosynthesis; D014407:Tumor Cells, Cultured / Q000378:metabolism
D019485:Bone Morphogenetic Proteins; D009363:Neoplasm Proteins; D010281:Parathyroid Hormone; D000242:Cyclic AMP; D000469:Alkaline Phosphatase