title: Effects of 3,3'-di-O-methylquercetin on guinea-pig isolated smooth muscle.
abstract: The effects of the flavone 3,3'-di-O-methylquercetin (DOMQ) have been examined and compared with those of quercetin, on guinea-pig isolated ileum, trachea, and main pulmonary artery (MPA). Except for transient contractions induced by low concentrations (10(-8)-3 x 10(-6) M), DOMQ and quercetin (up to 3 x 10(-4) M) caused reduction of the tone and the phasic contractions of the ileum. A23187 reversed the inhibitory effects of quercetin but not those of DOMQ. DOMQ and quercetin caused concentration-dependent relaxation of the trachea and the adrenaline-contracted MPA. DOMQ shifted to the right the concentration-effect curves induced by acetylcholine on the ileum and the trachea, and by adrenaline on MPA and those induced by CaCl2 on ileum, trachea and MPA. DOMQ also inhibited the contractions induced, in Ca2+-free EGTA-containing buffer, by histamine on ileum and by adrenaline on MPA. These observations suggest that DOMQ inhibits Ca2+ influx, Ca2+ release from intracellular stores and, more likely, Ca2+ binding to intracellular receptor proteins.
authors.affiliation: Department of Biological Sciences, Faculty of Science, University of Jordan, Amman.
authors.forename: S
authors.initials: S
authors.lastname: Abdalla
authors.forename: M A
authors.initials: MA
authors.lastname: Zarga
authors.forename: F
authors.initials: F
authors.lastname: Afifi
authors.forename: S
authors.initials: S
authors.lastname: al-Khalil
authors.forename: A
authors.initials: A
authors.lastname: Mahasneh
authors.forename: S
authors.initials: S
authors.lastname: Sabri
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D005419:Flavonoids / Q000494:pharmacology*; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology; D007082:Ileum / Q000187:drug effects; D066298:In Vitro Techniques; D008297:Male; D009126:Muscle Relaxation / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D011651:Pulmonary Artery / Q000187:drug effects; D011794:Quercetin / Q000031:analogs & derivatives / Q000494:pharmacology*; D014132:Trachea / Q000187:drug effects
chemical_list: D005419:Flavonoids; C060385:3,3'-di-O-methylquercetin; D006632:Histamine; D011794:Quercetin; D000109:Acetylcholine