[Effect of camptothecin on the DNA-relaxing and DNA-cleavage activity of calf thymus topoisomerase I].
Nucleotide sequences that are cleaved by calf thymus type I topoisomerase have been determined using cloned human Ha-ras and p53 genes. Localization and relative frequency of single-strand cleavages within these sequences were observed to change in the presence of the cytotoxic alkaloid camptothecin.
Bronshteĭn
I B
IB
Gromova
I I
II
Bukhman
V L
VL
Kafiani
K A
KA
D000818:Animals; D001483:Base Sequence; D002166:Camptothecin / Q000494:pharmacology*; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism*; D004264:DNA Topoisomerases, Type I / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D011905:Genes, ras; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids; D013950:Thymus Gland / Q000201:enzymology
D004247:DNA; D004264:DNA Topoisomerases, Type I; D002166:Camptothecin
Vliianie kamptotetsina na DNK-relaksiruiushchuiu i DNK-rasshchepliaiushchuiu aktivnosti topoizomerazy I iz timusa telenka.