[Comparative study of immunologic techniques applied to the detection of herpes simplex virus (HSV)].
Indirect immunofluorescence (IF), immunoperoxidase (IP) and seroneutralization tests were compared for detection and identification or Herpes simplex virus (HSV), directly in tissue smears or in cells inoculated with clinical materials. Detection and clear differentiation between the two HSV serotypes were obtained using rabbit immunoglobulin cross-absorbed with heterologous virus antigen. Immunofluorescent staining with specific monoclonal antibodies is as sensitive and more rapid than standard Vero cell cultures, for the laboratory diagnosis of HSV.
Meguenni
S
S
Bandoui
D
D
Lahlou
Y
Y
El Kolli
J
J
Benyakoub
D
D
Bouguermouh
A
A
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D005455:Fluorescent Antibody Technique; D006561:Herpes Simplex / Q000175:diagnosis / Q000382:microbiology*; D006801:Humans; D007124:Immunoenzyme Techniques; D009500:Neutralization Tests; D011237:Predictive Value of Tests; D018139:Simplexvirus / Q000145:classification / Q000302:isolation & purification*; D014709:Vero Cells / Q000382:microbiology; D014776:Virus Cultivation
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral
Etude comparative des techniques immunologiques appliquées a la détection des virus herpes simplex (HSV).