title: Blockade of the late IPSP in rat CA1 hippocampal neurons by 2-hydroxy-saclofen.
abstract: The effects of the GABAB receptor antagonist 2-hydroxy-saclofen were studied using intracellular recording of synaptic potential from CA1 hippocampal neurons. 2-Hydroxy-saclofen (50-200 microM) reversibly blocked the late, GABAB receptor-mediated inhibitory postsynaptic potential (IPSP) but not the early, GABAA receptor-mediated IPSP. In addition, the hyperpolarizing response to baclofen was reduced by similar concentrations of 2-hydroxy-saclofen. This suggests that 2-hydroxy-saclofen is a potent antagonist at postsynaptic GABAB receptors on hippocampal neurons.
authors.affiliation: Department of Neurobiology, Northeastern Ohio Universities College of Medicine, Rootstown 44272.
authors.forename: N A
authors.initials: NA
authors.lastname: Lambert
authors.forename: N L
authors.initials: NL
authors.lastname: Harrison
authors.forename: D I
authors.initials: DI
authors.lastname: Kerr
authors.forename: J
authors.initials: J
authors.lastname: Ong
authors.forename: R H
authors.initials: RH
authors.lastname: Prager
authors.forename: T J
authors.initials: TJ
authors.lastname: Teyler
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D001418:Baclofen / Q000031:analogs & derivatives* / Q000494:pharmacology; D058787:GABA-A Receptor Antagonists; D006624:Hippocampus / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D009433:Neural Inhibition / Q000187:drug effects*; D051381:Rats; D011963:Receptors, GABA-A / Q000502:physiology*
chemical_list: D058787:GABA-A Receptor Antagonists; D011963:Receptors, GABA-A; D001418:Baclofen; C057491:2-hydroxysaclofen