Destabilization of herpes simplex virus type 1 virions by local anesthetics, alkaline pH, and calcium depletion.
The infectivity of herpes simplex virus type 1 (HSV-1) was found to be markedly reduced by treating virions with the tertiary amine local anesthetics lidocaine, dibucaine and tetracaine. These treatments induced a characteristic shift in the buoyant density of the HSV-1 particles from a "light" to a "heavy" population. HSV-1 virions were unstable at alkaline pH, and alkali treatment caused the same shift in buoyant density. Ca2+ stabilized the "light" population. These results suggest that the physicochemical status of the HSV-1 envelope which is sensitive to the treatments described above plays an important role for the integrity of the virion.
Yanagi
K
K
Department of Virology and Rickettsiology, National Institute of Health, Tokyo, Japan.
Harada
S
S
D000779:Anesthetics, Local / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology*; D002213:Capsid / Q000187:drug effects / Q000502:physiology; D002499:Centrifugation, Density Gradient; D003992:Dibucaine / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008012:Lidocaine / Q000494:pharmacology; D008274:Magnesium / Q000494:pharmacology; D018139:Simplexvirus / Q000187:drug effects* / Q000254:growth & development; D013748:Tetracaine / Q000494:pharmacology; D014771:Virion / Q000187:drug effects*; D014775:Virus Activation / Q000187:drug effects
D000779:Anesthetics, Local; D013748:Tetracaine; D008012:Lidocaine; D008274:Magnesium; D003992:Dibucaine; D002118:Calcium