title: The translational requirement for complete La Crosse virus mRNA synthesis is cell-type dependent.
abstract: The translational requirement to prevent premature termination during La Crosse virus S mRNA synthesis was found to be cell-type dependent. This requirement was present in the BHK, HEL, and Vero cell lines we examined, but not in C6/36 mosquito cells. The cell-dependent translational requirement could be reproduced in vitro by using either cell extracts or purified virions of BHK and C6/36 cells. In the BHK reactions, the polymerase terminated predominantly at nucleotide 175 in the absence of concurrent translation and required translation to read through this position. In the C6/36 reactions, however, the polymerase reads through nucleotide 175 efficiently independent of translation. Reconstitution studies suggested that the translational requirement was due to a factor(s) present in BHK, but not in C6/36, cells.
authors.affiliation: Department of Microbiology, University of Geneva School of Medicine, Switzerland.
authors.forename: R
authors.initials: R
authors.lastname: Raju
authors.forename: L
authors.initials: L
authors.lastname: Raju
authors.forename: D
authors.initials: D
authors.lastname: Kolakofsky
mesh_terms: D000818:Animals; D002043:Bunyaviridae / Q000235:genetics*; D002460:Cell Line; D009033:Culicidae; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D002141:Encephalitis Virus, California / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis*; D011817:Rabbits; D014158:Transcription, Genetic; D014709:Vero Cells; D014771:Virion / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D012321:DNA-Directed RNA Polymerases