[A study on the inhibition of non-specific neutral proteinase by gold salt. Part 1. Gold sodium thiomalate].
The inhibitory effect of gold sodium thiomalate on non-specific neutral proteinase (EC 3.4.24.4) was investigated in vitro, in order to clarify the suppressive mechanism of the inflammatory process of joints by gold salt. The neutral proteinase was inhibited time- and concentration-dependently by gold salt. When the enzyme was incubated with 1 x 10(-2)M gold sodium thiomalate for 60 minutes, almost 100% of the activity was lost. Even at a lower concentration (1 x 10(-5)M, gold sodium thiomalate inhibited the enzyme time-dependently after longer incubation (3 or 9 hours). However, the same concentration of gold sodium thiomalate previously incubated with alpha-casein did not inhibit it. The results obtained here suggest that gold sodium thiomalate may inhibit the enzyme by binding it. The delay of neutral proteinase inhibition by gold sodium thiomalate seemed to be dependent on the concentration of gold salt.
Arita
S
S
Department of Orthopedic Surgery, Gunma University School of Medicine, Japan.
D018501:Antirheumatic Agents; D002364:Caseins / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006052:Gold Sodium Thiomalate / Q000494:pharmacology*; D011402:Pronase / Q000037:antagonists & inhibitors*; D013995:Time
D018501:Antirheumatic Agents; D002364:Caseins; D006052:Gold Sodium Thiomalate; D011402:Pronase