Chemical specificity of short-chain fatty acid-induced electrogenic secretory response in the rat colonic mucosa.
1. Luminal additions of normal short-chain fatty acids (SCFA: 3-6 carbons) increased transmural potential difference (PD: lumen negative) across rat everted colon in a dose-dependent manner. 2. The PD response to SCFA adapted rapidly and was not evoked by further cumulative addition of the same acid (self-adaptation) or different acids (cross-adaptation). 3. A broad range of SCFA derivatives were tested as potential stimulators of the PD response and cross-adaptation with propionate: a single carboxyl group and 3-6 carbon-chain were an absolute requirement for recognition. 4. These results suggest that SCFA-reception mechanism of the rat colonic mucosa has some sort of chemical specificity for stimulating electrogenic secretory response.
Yajima
T
T
Yakult Central Institute for Microbiological Research, Tokyo, Japan.
D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D003106:Colon / Q000378:metabolism*; D005227:Fatty Acids; D005232:Fatty Acids, Volatile / Q000494:pharmacology*; D006880:Hydroxy Acids / Q000494:pharmacology; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000378:metabolism*; D007651:Keto Acids / Q000494:pharmacology; D008297:Male; D008564:Membrane Potentials; D051381:Rats; D011919:Rats, Inbred Strains; D012636:Secretory Rate / Q000187:drug effects; D013329:Structure-Activity Relationship
D000596:Amino Acids; D005227:Fatty Acids; D005232:Fatty Acids, Volatile; D006880:Hydroxy Acids; D007651:Keto Acids