Immunocytochemical staining of astrocytes, tanycytes, immature glial cells and ependymal cells in the central nervous system of the normal rat and the myelin-deficient mutant.
Central nervous system (CNS) tissue from young myelin-deficient (md) mutant rats and male littermate controls was immunostained with antibodies against the following glial-cell antigens: glial fibrillary acidic protein (GFAP), glutamine synthetase, vimentin, the Yb isozyme of glutathione-S-transferase (Yb) and carbonic anhydrase. Gliosis was most severe in spinal-cord 'white-matter' tracts in the md rat. The most dramatic differences between astrocytes in the md and normal animals were revealed with anti-GFAP and anti-vimentin. Ependymal cells and tanycytes were quite similar in the md and normal CNS. Among the glial-cell antigens, Yb showed the broadest distribution, which included astrocytes, ependymal cells and tanycytes.
Cammer
W
W
Department of Neurology, Albert Einstein College of Medicine, Bronx, N.Y.
Tansey
F A
FA
D000818:Animals; D002490:Central Nervous System / Q000378:metabolism / Q000473:pathology*; D003711:Demyelinating Diseases / Q000473:pathology*; D004805:Ependyma / Q000378:metabolism / Q000473:pathology*; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D009457:Neuroglia / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D011919:Rats, Inbred Strains; D014746:Vimentin / Q000378:metabolism
D005904:Glial Fibrillary Acidic Protein; D014746:Vimentin; D005974:Glutamate-Ammonia Ligase