title: Interleukin 1 beta inhibition of TRH-stimulated prolactin secretion and phosphoinositides metabolism.
abstract: The effect of interleukin 1 beta on prolactin secretion and on phosphoinositide turnover in anterior pituitary cells was evaluated. Interleukin 1 beta significantly inhibited TRH-stimulated prolactin secretion assessed by the reverse hemolytic plaque assay. In particular, the cytokine reduced the percentage of plaque forming cells, the plaque mean area, the large plaques percentage. TRH-stimulated inositol phosphate production was also significantly inhibited by interleukin 1 beta. This study shows that interleukin 1 beta reduces TRH-induced prolactin secretion through a direct action on pituitary cell, and attenuates the TRH-stimulated phosphoinositide breakdown. This latter effect may suggest that the reduced lactotropes sensitivity to TRH action may be partially due to interleukin 1 beta inhibition of phosphatidylinositol breakdown.
authors.affiliation: Institute of Pharmacology, II School of Medicine, University of Naples, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Schettini
authors.forename: E
authors.initials: E
authors.lastname: Landolfi
authors.forename: M
authors.initials: M
authors.lastname: Grimaldi
authors.forename: O
authors.initials: O
authors.lastname: Meucci
authors.forename: A
authors.initials: A
authors.lastname: Postiglione
authors.forename: T
authors.initials: T
authors.lastname: Florio
authors.forename: C
authors.initials: C
authors.lastname: Ventra
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006462:Hemolytic Plaque Technique; D007295:Inositol Phosphates / Q000378:metabolism*; D007375:Interleukin-1 / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D010716:Phosphatidylinositols / Q000378:metabolism*; D010903:Pituitary Gland, Anterior / Q000187:drug effects / Q000378:metabolism*; D011388:Prolactin / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011994:Recombinant Proteins / Q000494:pharmacology; D013973:Thyrotropin-Releasing Hormone / Q000037:antagonists & inhibitors / Q000494:pharmacology*
chemical_list: D007295:Inositol Phosphates; D007375:Interleukin-1; D010716:Phosphatidylinositols; D011994:Recombinant Proteins; D013973:Thyrotropin-Releasing Hormone; D011388:Prolactin