title: Amino acid sequence of cytochrome c from Aspergillus niger.
abstract: Cytochrome c from Aspergillus niger consists of two forms, a major one (80%) with 111 amino acid residues and a minor one (20%) with 108 residues, missing the three N-terminal residues of the major one. The primary sequence of A. niger cytochrome c was determined by standard spinning-cup Edman degradation of purified peptides and of pairs of peptides, from which the desired sequence was readily deduced by subtraction of common sequencies. Except for the extension and some variability at the N-terminal sequence, the A. niger protein conforms well with other cytochrome c structures.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Texas Medical School, Houston 77225.
authors.forename: C C
authors.initials: CC
authors.lastname: Chin
authors.forename: W G
authors.initials: WG
authors.lastname: Niehaus
authors.forename: F
authors.initials: F
authors.lastname: Wold
mesh_terms: D000595:Amino Acid Sequence; D001234:Aspergillus niger / Q000378:metabolism*; D003488:Cyanogen Bromide; D003574:Cytochrome c Group; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis
chemical_list: D003574:Cytochrome c Group; D007202:Indicators and Reagents; D010446:Peptide Fragments; D003488:Cyanogen Bromide