The Epstein-Barr virus immediate-early gene product, BMLF1, acts in trans by a posttranscriptional mechanism which is reporter gene dependent.
In DNA cotransfection experiments, the Epstein-Barr virus immediate-early gene product, BMLF1, stimulated the chloramphenicol acetyltransferase (CAT) activity of both latent and productive EBV promoters linked to CAT. This BMLF1-induced increase in CAT activity was out of proportion to the effect on CAT mRNA, suggesting a posttranscriptional mechanism. Furthermore, when growth hormone was used as a reporter gene instead of CAT, BMLF1 no longer functioned. Thus, the BMLF1 effect was reporter-gene dependent. The effect of the BMLF1 gene product does not then appear to be directed at promoter activation, but instead may function to increase the level of an as yet unidentified protein(s) required for Epstein-Barr virus infection.
Kenney
S
S
Lineberger Cancer Research Center, University of North Carolina, Chapel Hill 27514.
Kamine
J
J
Holley-Guthrie
E
E
Mar
E C
EC
Lin
J C
JC
Markovitz
D
D
Pagano
J
J
D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D015696:Gene Products, tat; D013006:Growth Hormone / Q000235:genetics; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis; D014157:Transcription Factors / Q000502:physiology*; D014764:Viral Proteins / Q000502:physiology*
D015696:Gene Products, tat; D012333:RNA, Messenger; D014157:Transcription Factors; D014764:Viral Proteins; D013006:Growth Hormone; D015500:Chloramphenicol O-Acetyltransferase