Rapid diagnosis of adenovirus pharyngoconjunctival fever: use of a monoclonal antibody-based ELISA test during an outbreak.
A monoclonal antibody-based solid-phase immunoassay (ELISA) was used to detect adenovirus antigen in clinical specimens during an outbreak of pharyngoconjunctival fever at a boarding school. The use of the kit enabled rapid diagnosis of the cause of the outbreak. The assay was also valuable in confirmation of tissue culture isolation of adenovirus. This kit could be of value to laboratories without tissue culture facilities and as an alternative to immunofluorescence for adenovirus confirmation in laboratories which have such facilities.
Player
V
V
Microbiology/Public Health Laboratory, Royal United Hospital, Combe Park, Bath, U.K.
Westmoreland
D
D
D000257:Adenoviridae Infections / Q000175:diagnosis*; D000258:Adenovirus Infections, Human / Q000175:diagnosis* / Q000453:epidemiology; D000260:Adenoviruses, Human / Q000276:immunology / Q000302:isolation & purification; D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis*; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D004196:Disease Outbreaks; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D011933:Reagent Kits, Diagnostic