title: Coupling of the enzymic activities of myosin ATPase and creatine kinase and its role in muscular contraction.
abstract: During muscular contraction the regeneration of ATP, catalysed by creatine kinase (CK), keeps pace with the hydrolysis of ATP by myosin ATPase posing the question of its regulatory mechanism. In the background of F-actin activation of heavy meromyosin (HMM) ATPase activity we have investigated in vitro the role of F-actin in regulating CK's activity in the absence and presence of HMM. For the coupled enzyme system we have also looked into the roles played by the individual reactants. F-actin has been found to appreciably increase CK's activity in the absence of HMM. While HMM alone inhibited CK's activity, there was a several fold increase when F-actin was also present. By a process of elimination we conclude that none of the reactants apart from H+ could be involved in regulating CK's activity in the coupled enzyme system. As no change in the pH of reaction mixture was observed during the reaction, we further conclude that the two enzymic reactions are coupled by proton transfer along F-actin. Implications of the findings for PCr-Cr shuttle and movements of ATP and ADP in sarcomere are discussed.
authors.forename: L A
authors.initials: LA
authors.lastname: Khan
authors.forename: M
authors.initials: M
authors.lastname: Raj
authors.forename: M
authors.initials: M
authors.lastname: Amin
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D003402:Creatine Kinase / Q000378:metabolism*; D007700:Kinetics; D008956:Models, Chemical; D009119:Muscle Contraction / Q000502:physiology*; D009218:Myosins / Q000378:metabolism*; D011522:Protons; D011817:Rabbits
chemical_list: D000199:Actins; D011522:Protons; D003402:Creatine Kinase; D009218:Myosins