Correlation between neutrophil superoxide formation, luminol-augmented chemiluminescence and intracellular Ca2+ levels upon stimulation with leukotriene B4, formylpeptide and phorbolester.
When neutrophils were stimulated with leukotriene B4 (LTB4) .O2- -production and elevation of intracellular Ca2+ were initiated more rapidly than after stimulation with N-formyl-methionyl-leucyl-phenylalanine. Phorbol myristate acetate (PMA) induced a substantially more protracted superoxide (.O-2) production. Luminol augmented chemiluminescence (LCL) reflected the kinetic characteristics accurately. The primary distinguishing attribute of LTB4-induced responses were an initial high rate of activation and a rapid termination. Thus, LTB4-induced neutrophil Ca2+-inflow and .O2- -formation may be initiated by mechanisms similar in part to those for formylpeptides; however it does not possess the ability to maintain this activation. The kinetic properties of neutrophil activation may be studied with LCL, irrespective of the exact nature of the LCL-generating oxidants formed by neutrophils.
Gyllenhammar
H
H
Department of Medicine 3, Karolinska Institute at Södersjukhuset, Stockholm, Sweden.
D002118:Calcium / Q000378:metabolism*; D006801:Humans; D007975:Leukotriene B4 / Q000494:pharmacology*; D008163:Luminescent Measurements; D008165:Luminol / Q000494:pharmacology*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D009504:Neutrophils / Q000378:metabolism*; D011724:Pyridazines / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D011724:Pyridazines; D013481:Superoxides; D007975:Leukotriene B4; D009240:N-Formylmethionine Leucyl-Phenylalanine; D008165:Luminol; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium