Molecular species of the antitumor and antiviral fraction from pine cone extract.
The most active fraction (Fr. VI) of antitumor and antiviral pine cone extract was subjected to chemical structure analysis. IR, UV, and ESR spectroscopies, in addition to chromatographic analysis on cellulose-TLC plates, reveal that the major component of Fr. VI includes structures belonging to the lignin family. A commercial alkali-lignin showed antiviral activity and granulocyte iodination stimulating activity as effective as those of Fr. VI, but had much weaker immunopotentiating activities, such as antitumor and antimicrobial activities and splenocyte stimulating activity. As a tentative conclusion, the active principle of Fr. VI might be complex(s) of lignin-like polyphenolic skeleton with a molecular matrix of unknown components.
Sakagami
H
H
First Department of Biochemistry, School of Medicine, Showa University, Tokyo, Japan.
Oh-Hara
T
T
Kaiya
T
T
Kawazoe
Y
Y
Nonoyama
M
M
Konno
K
K
D000818:Animals; D000890:Anti-Infective Agents / Q000302:isolation & purification* / Q000627:therapeutic use; D000970:Antineoplastic Agents / Q000302:isolation & purification*; D000998:Antiviral Agents / Q000302:isolation & purification*; D002460:Cell Line; D004261:DNA Replication; D004578:Electron Spin Resonance Spectroscopy; D004927:Escherichia coli Infections / Q000188:drug therapy; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008826:Microbial Sensitivity Tests; D009504:Neutrophils / Q000187:drug effects; D009975:Orthomyxoviridae / Q000187:drug effects; D010936:Plant Extracts / Q000032:analysis* / Q000494:pharmacology / Q000627:therapeutic use; D018139:Simplexvirus / Q000187:drug effects; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet; D014197:Trees
D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D000998:Antiviral Agents; D010936:Plant Extracts