title: The human dopamine D2 receptor gene is located on chromosome 11 at q22-q23 and identifies a TaqI RFLP.
abstract: Human dopaminergic neurons are involved in the control of hormone secretion, voluntary movement, and emotional behavior. Mediating these effects are the dopamine D1 and D2 receptors. These macromolecules belong to a large family of related sequences known as the G protein-coupled receptors. The D2 receptors have been of special interest because they bind, with high affinity and specificity, many of the commonly prescribed antipsychotic drugs. We previously isolated a full-length cDNA clone of the rat D2 receptor. When a chromosome mapping panel was probed with the rat D2 receptor cDNA a 15-kb EcoRI restriction fragment was identified and localized to human chromosome 11. The rat cDNA was also used to clone a human genomic fragment, lambda hD2G1, which contains the last coding exon of the D2 receptor gene (DRD2) and 16.5 kb of 3' flanking sequence. Hybridization of lambda hD2G1 to a chromosome 11 regional mapping panel localized DRD2 to 11q. In situ hybridization of lambda hD2G1 to metaphase chromosomes refined this assignment to the q22-q23 junction of chromosome 11. A search for RFLPs associated with D2DR identified a frequent two-allele TaqI RFLP.
authors.affiliation: Vollum Institute for Advanced Biomedical Research, Oregon Health Sciences University, Portland, 97201.
authors.forename: D K
authors.initials: DK
authors.lastname: Grandy
authors.forename: M
authors.initials: M
authors.lastname: Litt
authors.forename: L
authors.initials: L
authors.lastname: Allen
authors.forename: J R
authors.initials: JR
authors.lastname: Bunzow
authors.forename: M
authors.initials: M
authors.lastname: Marchionni
authors.forename: H
authors.initials: H
authors.lastname: Makam
authors.forename: L
authors.initials: L
authors.lastname: Reed
authors.forename: R E
authors.initials: RE
authors.lastname: Magenis
authors.forename: O
authors.initials: O
authors.lastname: Civelli
mesh_terms: D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D003001:Cloning, Molecular; D015252:Deoxyribonucleases, Type II Site-Specific; D006801:Humans; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length; D011954:Receptors, Dopamine / Q000235:genetics*; D017448:Receptors, Dopamine D2
chemical_list: D011954:Receptors, Dopamine; D017448:Receptors, Dopamine D2; D015252:Deoxyribonucleases, Type II Site-Specific; C027046:TCGA-specific type II deoxyribonucleases