[Three cases of vulvar bowenoid papulosis: the localization of HPV DNA by in situ hybridization].
Cytological, histological, and molecular biological studies were conducted in 3 cases of vulvar Bowenoid papulosis, using biotinylated HPV DNA probes by in situ hybridization. 1) Cytological findings showed dyskaryotic cells that revealed hyperchromatism with a coarse granular pattern, and a high N/C ratio was observed among the dyskeratotic cells. 2) In 2 cases of Bowenoid papulosis lesions, HPV 16 DNA was detected in the nucleus of the dysplastic cells. 3) In one case of Bowenoid papulosis, a complicated carcinoma in situ of the uterine cervix was observed, and the HPV 16 DNA was found to be positive in both the vulva and cervix.
Kioka
H
H
Dept. of Obstet. & Gynec. Hiroshima Univ. School of Med.
Nagai
N
N
Tanioka
Y
Y
Fujii
T
T
Katsube
Y
Y
Egawa
K
K
Fujiwara
A
A
D000328:Adult; D001913:Bowen's Disease / Q000032:analysis* / Q000473:pathology; D002278:Carcinoma in Situ / Q000032:analysis / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000032:analysis* / Q000473:pathology; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D009378:Neoplasms, Multiple Primary / Q000032:analysis; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification; D002583:Uterine Cervical Neoplasms / Q000032:analysis / Q000473:pathology; D014846:Vulvar Neoplasms / Q000032:analysis* / Q000473:pathology
D015342:DNA Probes; D004279:DNA, Viral