[Scavenging effects of probimane on active oxygen free radicals by electron spin resonance].
Probimane, dl-1,2-bis (4-morpholine-methyl-3, 5-dioxopiperazin-1-yl) propane, is a new antitumor agent synthesized by Shanghai Institute of Materia Medica, Chinese Academy of Sciences. The scavenging effects of probimane on active oxygen radicals produced in 3 different systems were studied with the ESR spin trapping methods. In Fenton's reaction, probimane remarkably scavenged hydroxyl radicals (.OH) and the rate of scavenging .OH by probimane 0.05 mmol/L was 47%, compared to 5% by vitamin E (VE) and 30% by ascorbic acid (AA). In irradiation riboflavin system, in which superoxide (O2-.) was produced, the agent also had the scavenging effects on O2(-.). The rate of scavenging O2-. by probimane 0.05 mmol/L was 13%, higher than that by VE (7%) but lower than that by AA (90%). In cell system where the active oxygen radicals were produced during the respiratory burst of human neutrophils (Neu) stimulated by TPA (tetradecanoylphorbol acetate), probimane exhibited a dose-dependent scavenging action on the radicals. The rate of the radical scavenging by probimane 0.05 mmol/L was 37%, much higher than that by VE (9%) but lower than that by AA (68%). Probimane had no effect on the rate of oxygen consumption by human Neu, measured with spin probe oxymetry.
Dan
N
N
Li
X J
XJ
Zhao
B L
BL
Zhang
T M
TM
Xin
W J
WJ
D000970:Antineoplastic Agents; D001205:Ascorbic Acid / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D006801:Humans; D009504:Neutrophils / Q000378:metabolism; D010101:Oxygen Consumption / Q000187:drug effects*; D010879:Piperazines / Q000494:pharmacology*; D011929:Razoxane / Q000031:analogs & derivatives / Q000494:pharmacology*; D014810:Vitamin E / Q000494:pharmacology
D000970:Antineoplastic Agents; D005609:Free Radicals; D010879:Piperazines; C055262:probimane; D014810:Vitamin E; D011929:Razoxane; D001205:Ascorbic Acid