title: Endocytosis of simian virus 40 into the endoplasmic reticulum.
abstract: The endocytosis of SV-40 into CV-1 cells we studied using biochemical and ultrastructural techniques. The half-time of binding of [35S]methionine-radiolabeled SV-40 to CV-1 cells was 25 min. Most of the incoming virus particles remained undegraded for several hours. Electron microscopy showed that some virus entered the endosomal/lysosomal pathway via coated vesicles, while the majority were endocytosed via small uncoated vesicles. After infection at high multiplicity, one third of total cell-associated virus was observed to enter the ER, starting 1-2 h after virus application. The viruses were present in large, tubular, smooth membrane networks generated as extentions of the ER. The results describe a novel and unique membrane transport pathway that allows endocytosed viral particles to be targeted from the plasma membrane to the ER.
authors.affiliation: Department of Cell Biology, Yale School of Medicine, New Haven, Connecticut 06510.
authors.forename: J
authors.initials: J
authors.lastname: Kartenbeck
authors.forename: H
authors.initials: H
authors.lastname: Stukenbrok
authors.forename: A
authors.initials: A
authors.lastname: Helenius
mesh_terms: D000818:Animals; D001345:Autoradiography; D002460:Cell Line; D004705:Endocytosis; D004721:Endoplasmic Reticulum / Q000378:metabolism* / Q000382:microbiology / Q000648:ultrastructure; D007124:Immunoenzyme Techniques; D007700:Kinetics; D008715:Methionine / Q000378:metabolism; D008854:Microscopy, Electron; D013539:Simian virus 40 / Q000378:metabolism* / Q000648:ultrastructure; D013462:Sulfur Radioisotopes
chemical_list: D013462:Sulfur Radioisotopes; D008715:Methionine