title: Double antigen localization of two catecholamine enzymes and GABA in amacrine cells of the rat retina in semi-thin sections.
abstract: A double immunohistochemical labeling technique was developed with two immunoperoxidase reactions performed before and after embedding in epoxy resin using two different chromogens: diaminobenzidine (yellowish brown color) and diaminobenzidine + nickel ammonium sulfate (black color). The two catecholamine enzyme immunoreactivities (phenylethanolamine-N-methyltransferase, and tyrosine-hydroxylase) were not found in the same cells, while gamma-aminobutyric acid (GABA) immunoreactivity was observed in large tyrosine-hydroxylase immunoreactive cells, but not in phenylethanolamine-N-methyltransferase immunoreactive cells.
authors.affiliation: INSERM U 86-Laboratoire de Neurocytologie Oculaire, Paris, France.
authors.forename: A
authors.initials: A
authors.lastname: Simon
authors.forename: C
authors.initials: C
authors.lastname: Versaux-Botteri
authors.forename: L
authors.initials: L
authors.lastname: Denoroy
authors.forename: A
authors.initials: A
authors.lastname: Vigny
authors.forename: J
authors.initials: J
authors.lastname: Nguyen-Legros
mesh_terms: D015100:3,3'-Diaminobenzidine; D000818:Animals; D002395:Catecholamines / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods*; D010625:Phenylethanolamine N-Methyltransferase / Q000378:metabolism*; D051381:Rats; D012160:Retina / Q000166:cytology / Q000378:metabolism*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D002395:Catecholamines; D015100:3,3'-Diaminobenzidine; D005680:gamma-Aminobutyric Acid; D014446:Tyrosine 3-Monooxygenase; D010625:Phenylethanolamine N-Methyltransferase