raf involvement in the simultaneous genetic transfer of the radioresistant and transforming phenotypes.
We examined a human Alu+ mouse tertiary transformant derived from a noncancerous skin fibroblast cell line which exhibits the unique characteristic of being resistant to the killing effects of ionizing radiation. This transformed cell line was found to contain activated human c-raf-1, and demonstrated an increased level of radioresistance indicating the simultaneous transfer of both the transforming and radiation-resistant phenotypes. We have also found a relationship between the presence of activated oncogenes, specifically those with serine/threonine kinase activity and the radioresistant phenotype.
Pirollo
K F
KF
Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814.
Garner
R
R
Yuan
S Y
SY
Li
L
L
Blattner
W A
WA
Chang
E H
EH
D000818:Animals; D002461:Cell Line, Transformed; D006801:Humans; D051379:Mice; D009857:Oncogenes; D010641:Phenotype; D011836:Radiation Tolerance; D014162:Transfection; D014170:Transformation, Genetic