Confirmation and refinement of the localisation of the c-MEL locus on chromosome 19 by physical and genetic mapping.
The human gene locus c-MEL was identified following transfection of genomic DNA from the human melanoma cell line NK14; it has previously been assigned to chromosome 19 (p13.2-q13.2) by analysis of somatic cell hybrids. We have further refined the position of this gene to the proximal region of 19p (cen-p13.2), using cell hybrids containing only fragments of human chromosome 19. We have confirmed this physical localisation by linkage analysis with a recently described restriction fragment length polymorphism for the c-MEL gene, and mapped the locus within the region of the low density lipoprotein receptor gene (LDLR) (Lod 4.43, theta = 0.10) and the anonymous marker D19S11 (13.1.25) (Lod 9.33, theta = 0). This gene thus maps to a region of chromosome 19 involved in karyotypic abnormalities in a variety of malignancies including melanomas and leukaemias.
Nimmo
E
E
Department of Biochemistry and Molecular Genetics, St. Mary's Hospital Medical School, Paddington, London, UK.
Padua
R A
RA
Hughes
D
D
Brook
J D
JD
Williamson
R
R
Johnson
K J
KJ
D002460:Cell Line; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19; D015342:DNA Probes; D005796:Genes; D008040:Genetic Linkage; D006801:Humans; D006822:Hybrid Cells; D012150:Polymorphism, Restriction Fragment Length; D014162:Transfection
D015342:DNA Probes