Liquid chromatographic determination of vitamin D in infant formula.
A method is described for the determination of vitamins D2 + D3 in milk- and soy-based infant formula. Vitamins D2 and D3 are extracted from the saponified sample and converted to isotachysterols with acidified butanol. Reverse-phase liquid chromatography (LC) is used to remove interferences, and total vitamin D is quantitated using normal-phase LC. Recoveries of spiked samples averaged 97.6% for milk-based infant formula, and 98.8% for soy-based infant formula. This method quantitates vitamin D2 + D3 in infant formulas containing as low as 40 IU/qt when prepared according to label direction.
Agarwal
V K
VK
Connecticut Agricultural Experiment Station, New Haven 06504.
D002762:Cholecalciferol / Q000032:analysis; D002853:Chromatography, Liquid; D004872:Ergocalciferols / Q000032:analysis; D007202:Indicators and Reagents; D007225:Infant Food / Q000032:analysis*; D007536:Isomerism; D012997:Solvents; D014807:Vitamin D / Q000032:analysis*
D004872:Ergocalciferols; D007202:Indicators and Reagents; D012997:Solvents; D014807:Vitamin D; D002762:Cholecalciferol