Neurotransmitter-lymphocyte interactions: dual receptor modulation of lymphocyte proliferation and cAMP production.
Stimulation of the beta-adrenergic receptor on lymphocytes can decrease the proliferative response of these cells to mitogens. We have found that simultaneous stimulation of T cells with the beta-adrenergic agonist isoproterenol and mitogens (phytohemagglutinin (PHA) and OKT3 monoclonal antibody) results in a 2- to 4-fold increase in cAMP production compared to cells exposed to isoproterenol alone. Mitogens alone have little effect on cAMP synthesis, but do activate the phosphatidylinositol (PI) cycle, suggesting that interactions may be occurring between the second messenger systems resulting in a cAMP synergy. Further experiments suggest that calcium may be involved in inducing the cAMP synergy observed in T cells. It is proposed that the synergy between beta-adrenergic and mitogenic stimulation of T cells for cAMP may be involved in the mechanism of catecholamine modulation of lymphocyte function.
Carlson
S L
SL
Department of Microbiology and Immunology, University of Kentucky College of Medicine, Lexington 40536-0084.
Brooks
W H
WH
Roszman
T L
TL
D000242:Cyclic AMP / Q000096:biosynthesis*; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D015759:Ionomycin / Q000494:pharmacology; D007545:Isoproterenol / Q000276:immunology / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000276:immunology*; D018377:Neurotransmitter Agents / Q000276:immunology* / Q000378:metabolism; D010835:Phytohemagglutinins; D011943:Receptors, Adrenergic, beta / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D018377:Neurotransmitter Agents; D010835:Phytohemagglutinins; D011943:Receptors, Adrenergic, beta; D015759:Ionomycin; D000242:Cyclic AMP; D007545:Isoproterenol; D013755:Tetradecanoylphorbol Acetate