The membrane form of guanylate cyclase. Homology with a subunit of the cytoplasmic form of the enzyme.
A cDNA clone for the membrane form of guanylate cyclase has been isolated from the testis of the sea urchin Strongylocentrotus purpuratus. An open reading frame predicts a protein of 1125 amino acids including an apparent signal peptide of 21 residues; a single transmembrane domain of 25 amino acids divided the mature protein into an amino-terminal, extracellular domain of 485 amino acids and a carboxyl domain of 594 intracellular amino acids. Three potential Asn-linked glycosylation sites were present in the proposed extracellular domain. The deduced protein sequence was homologous to the protein kinase family and contained limited but significant regions of identity with a low molecular weight atrial natriuretic peptide receptor. The carboxyl region (202 amino acids) was 42% identical with a subunit of the cytoplasmic form of guanylate cyclase recently cloned from bovine lung (Koesling, D., Herz, J., Gausepohl, H., Niroomand, F., Hinsch, K.-D., Mulsch, A., Bohme, E., Schultz, G., and Frank, R. (1988) FEBS Lett. 239, 29-34). Therefore, the membrane form of guanylate cyclase is a member of an apparently large family of proteins that includes the low molecular weight atrial natriuretic peptide receptor, the soluble form of guanylate cyclase and protein kinases.
Thorpe
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Department of Molecular Physiology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
Garbers
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D000595:Amino Acid Sequence; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000235:genetics; D001483:Base Sequence; D015152:Blotting, Northern; D002462:Cell Membrane / Q000201:enzymology*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D006162:Guanylate Cyclase / Q000235:genetics*; D008969:Molecular Sequence Data; D012617:Sea Urchins; D012995:Solubility; D013045:Species Specificity
D009320:Atrial Natriuretic Factor; D004247:DNA; D006162:Guanylate Cyclase