Studies of the metabolism of human breast cancer spheroids by NMR.
Spheroids present a model for tumor behavior in vivo. In NMR studies, a problem of spheroid adhesion occurs. Here, we present a method which overcomes this problem and show the variations in phosphate metabolite concentrations as well as the rate of glucose metabolism as a function of spheroid age.
Ronen
S
S
Isotope Department, Weizmann Institute of Science, Rehovot, Israel.
Degani
H
H
D000255:Adenosine Triphosphate / Q000378:metabolism; D001943:Breast Neoplasms / Q000378:metabolism*; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000378:metabolism*; D005260:Female; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D010710:Phosphates / Q000378:metabolism; D014407:Tumor Cells, Cultured
D010710:Phosphates; D000255:Adenosine Triphosphate