Improved screening method for beta-blockers in urine using solid-phase extraction and capillary gas chromatography-mass spectrometry.
An improved screening method for beta-blockers in urine is proposed, involving enzymatic hydrolysis, solid-phase extraction and capillary gas chromatography-mass spectrometry. Several extraction methods for beta-blockers, such as conventional liquid-liquid and solid-phase extraction procedures, have been evaluated for at least eight beta-blockers. Additionally, the gas chromatographic properties and mass fragmentation of the trimethylsilyl-trifluoroacetyl, trifluoroacetyl and cyclic n-butylboronate derivatives of beta-blockers have been compared and evaluated with respect to their efficiency for screening urine. The resulting screening method proved to be a specific and sensitive procedure, enabling these analytes to be detected and identified up to 48 h after the administration of a dosage, usually encountered in doping cases.
Leloux
M S
MS
Netherlands Institute for Drugs and Doping Research, University of Utrecht, The Netherlands.
de Jong
E G
EG
Maes
R A
RA
D000319:Adrenergic beta-Antagonists / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D006868:Hydrolysis
D000319:Adrenergic beta-Antagonists