Effect of pyridoxamine on semicarbazide-sensitive amine oxidase activity of rabbit lung and heart.
Rabbit lung and heart show clorgyline-resistant benzylamine oxidase activity which is sensitive to semicarbazide (SSAO) and alpha-amino-guanidine. This SSAO activity is inhibited by pyridoxamine with an IC50 of 6.3 x -6 M for lung and of 1.1 x 10(-5) M for heart, the inhibition being non-competitive and only partially reversed by dialysis at 4 degrees C. Semicarbazide, alpha-aminoguanidine and pyridoxamine show a similar time-dependent type of inhibition of rabbit lung and heart SSAO.
Buffoni
F
F
Department of Pharmacology, University of Florence, Italy.
Banchelli
G
G
Bertocci
B
B
Raimondi
L
L
D006631:Amine Oxidase (Copper-Containing) / Q000378:metabolism; D000818:Animals; D001595:Benzylamine Oxidase / Q000037:antagonists & inhibitors / Q000378:metabolism; D003010:Clorgyline / Q000494:pharmacology; D003956:Dialysis; D006321:Heart / Q000187:drug effects; D066298:In Vitro Techniques; D008168:Lung / Q000187:drug effects / Q000201:enzymology*; D009124:Muscle Proteins / Q000378:metabolism; D009206:Myocardium / Q000201:enzymology*; D010084:Oxidation-Reduction; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010627:Phenethylamines / Q000378:metabolism; D011733:Pyridoxamine / Q000494:pharmacology*; D011817:Rabbits; D012663:Semicarbazides / Q000494:pharmacology*
D009124:Muscle Proteins; D010627:Phenethylamines; D012663:Semicarbazides; D011733:Pyridoxamine; D001595:Benzylamine Oxidase; D006631:Amine Oxidase (Copper-Containing); D000587:Oxidoreductases Acting on CH-NH Group Donors; D003010:Clorgyline