title: Structural alteration of the insulin-like growth factor II-gene in Wilms tumour.
abstract: In this study messenger ribonucleic acid (mRNA) and DNA of five Wilms tumours were investigated. As expected, the level of insulin-like growth factor (IGF) II-mRNA was elevated up to 50 times in tumour tissue as compared to normal adjacent kidney tissue. In addition, genomic DNA was isolated and digested with appropriate restriction enzymes. Southern blots were prepared and hybridized to IGF II-cDNA probes. Additional bands were present in one of the five Wilms tumours compared to normal tissue. The results indicate a rearrangement of the IGF II-gene on one of the two chromosomes. It is speculated, that this change is responsible for the elevated IGF II expression which may be a factor contributing to tumour growth.
authors.affiliation: Department of Biochemistry, University of Zürich, Switzerland.
authors.forename: J C
authors.initials: JC
authors.lastname: Irminger
authors.forename: E J
authors.initials: EJ
authors.lastname: Schoenle
authors.forename: J
authors.initials: J
authors.lastname: Briner
authors.forename: R E
authors.initials: RE
authors.lastname: Humbel
mesh_terms: D002675:Child, Preschool; D015321:Gene Rearrangement; D006133:Growth Substances / Q000235:genetics*; D006801:Humans; D007223:Infant; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D013002:Somatomedins / Q000235:genetics*; D009396:Wilms Tumor / Q000235:genetics*
chemical_list: D006133:Growth Substances; D013002:Somatomedins; D007335:Insulin-Like Growth Factor II