Rapid liquid chromatographic analysis of mitoxantrone in plasma with C18 sample purification.
A useful and rapid method for the analysis of mitoxantrone in human plasma is described. We purified the sample with a C18 Sep Pak cartridge pre-treated with methanol, water and 0.05 M ammonium phosphate (pH = 2.75). The drug and internal standard (Methylene Blue) were eluted from the cartridge with 1 M acetic acid in methanol and were separated on a 10 microns particle size CN Resolve cartridge in conjunction with a radial compression liquid chromatograph. The mobile phase consisted of a mixture of 0.05 M ammonium phosphate, acetonitrile and methanol (60:35:5, by vol), and the detection was performed spectrophotometrically at 660 nm. The peak height ratio (drug/internal standard) varied linearly (r greater than 0.993) with concentration over the range examined 0.01-3 micrograms ml-1, and the inter- and intra-run precision at high, medium and low concentrations were good; CV ranged from 2.52 to 7.2%. There was no interference from other anticancer drugs or analgesics. We applied the described method to investigate the pharmacokinetics of mitoxantrone using a rabbit as an in vivo model.
el-Yazigi
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Department of Biological and Medical Research, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
Yusuf
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D000818:Animals; D002853:Chromatography, Liquid; D006801:Humans; D008942:Mitoxantrone / Q000097:blood* / Q000493:pharmacokinetics; D011817:Rabbits; D012015:Reference Standards; D015203:Reproducibility of Results; D012822:Silicon Dioxide; D013997:Time Factors
D012822:Silicon Dioxide; C042953:octadecyl silane-bonded silica; D008942:Mitoxantrone