Changes in the restriction endonuclease patterns of four modified-live infectious bovine rhinotracheitis virus (IBRV) vaccines after one passage in host animal.
Four genomically different bovine herpes virus 1 (BHV 1) vaccines were used to inoculate BHV 1-negative heifers. Restriction endonuclease analysis (REA) of virus isolates from animals during acute infection, after reactivation of virus from latency, and after reactivation followed by superinfection, showed that changes occurred in the BHV 1 genome after one passage in the host animal. Furthermore, the changes varied according to the tissue from which virus was isolated. Southern probe analyses identified the left terminus of the unique long fragment and the inverted repeats as regions of the genome where variability was most often observed. It was concluded that, when REA is used in the diagnostic differentiation of BHV 1 isolates, caution must be applied in the interpretation of results.
Whetstone
C
C
National Animal Disease Center, USDA-Agricultural Research Service, Ames, Iowa 50010.
Miller
J
J
Bortner
D
D
Van der Maaten
M
M
D000818:Animals; D015139:Blotting, Southern; D002417:Cattle; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis*; D003907:Dexamethasone / Q000494:pharmacology; D005814:Genes, Viral; D007242:Herpesvirus 1, Bovine / Q000235:genetics*; D014611:Vaccination; D014765:Viral Vaccines / Q000235:genetics*; D014775:Virus Activation
D004279:DNA, Viral; D014765:Viral Vaccines; D003907:Dexamethasone; D004262:DNA Restriction Enzymes