title: Predominance of Borrelia burgdorferi specific B cells in cerebrospinal fluid in neuroborreliosis.
abstract: A nitrocellulose immunospot assay that allows the counting of cells secreting IgG, IgA, or IgM antibodies to Borrelia burgdorferi was used to compare B cell response to B burgdorferi at the cellular level in cerebrospinal fluid (CSF) and blood from patients with neuroborreliosis with that in patients with aseptic meningoencephalitis (AM) or non-inflammatory neurological diseases. 13 of the 14 patients with untreated neuroborreliosis had CSF cells secreting IgG antibodies to B burgdorferi (mean 17 cells per 10(4) CSF cells), whereas 8 of 12 patients examined had cells secreting IgA antibodies (mean 6 cells) and 10 of 12 had cells secreting IgM antibodies (mean 6 cells) per 10(4) CSF cells. IgG antibody producing cells predominated except in 2 patients with mainly or only IgM secreting cells. Cells secreting antibodies to B burgdorferi were rarely found in the blood and then at very low numbers, which reflects preferential compartmentalisation of the specific B cell response to the CSF. The cells were not detectable in CSF or blood from the two control groups. Evaluation of humoral immunity at the cellular level is a novel approach to the detection and localisation of immune events in neuroinflammatory disorders.
authors.affiliation: Department of Neurology, Karolinska Institutet, Huddinge University Hospital, Stockholm, Sweden.
authors.forename: S
authors.initials: S
authors.lastname: Baig
authors.forename: T
authors.initials: T
authors.lastname: Olsson
authors.forename: H
authors.initials: H
authors.lastname: Link
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000134:cerebrospinal fluid*; D001402:B-Lymphocytes / Q000276:immunology*; D001898:Borrelia / Q000276:immunology*; D001899:Borrelia Infections / Q000097:blood / Q000134:cerebrospinal fluid* / Q000276:immunology; D005260:Female; D006801:Humans; D007132:Immunoglobulin Isotypes / Q000134:cerebrospinal fluid; D008297:Male; D008875:Middle Aged; D009422:Nervous System Diseases / Q000276:immunology* / Q000382:microbiology
chemical_list: D000907:Antibodies, Bacterial; D007132:Immunoglobulin Isotypes