title: Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector.
abstract: A P-element vector has been constructed and used to generate lines of flies with single autosomal P-element insertions. The lines were analyzed in two ways: (1) the identification of cis-acting patterning information within the Drosophila genome, as revealed by a lacZ reporter gene within the P element, and (2) the isolation of lethal mutations. We examined 3768 independent lines for the expression of lacZ in embryos and looked among these lines for lethal mutations affecting embryonic neurogenesis. This type of screen appears to be an effective way to find new loci that may play a role in the development of the Drosophila nervous system.
authors.affiliation: Howard Hughes Medical Institute, San Francisco, California.
authors.forename: E
authors.initials: E
authors.lastname: Bier
authors.forename: H
authors.initials: H
authors.lastname: Vaessin
authors.forename: S
authors.initials: S
authors.lastname: Shepherd
authors.forename: K
authors.initials: K
authors.lastname: Lee
authors.forename: K
authors.initials: K
authors.lastname: McCall
authors.forename: S
authors.initials: S
authors.lastname: Barbel
authors.forename: L
authors.initials: L
authors.lastname: Ackerman
authors.forename: R
authors.initials: R
authors.lastname: Carretto
authors.forename: T
authors.initials: T
authors.lastname: Uemura
authors.forename: E
authors.initials: E
authors.lastname: Grell
mesh_terms: D000818:Animals; D005075:Biological Evolution; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000032:analysis; D005786:Gene Expression Regulation; D005804:Genes, Lethal; D005822:Genetic Vectors; D009154:Mutation; D009420:Nervous System / Q000196:embryology; D011993:Recombinant Fusion Proteins / Q000032:analysis; D001616:beta-Galactosidase / Q000032:analysis
chemical_list: D004251:DNA Transposable Elements; D011993:Recombinant Fusion Proteins; D001616:beta-Galactosidase