The germline repertoire of T cell receptor beta-chain genes in patients with chronic progressive multiple sclerosis.
The T cell receptor (TcR) beta-chain germline gene repertoire of multiple sclerosis (MS) patients was compared to that of 100 normal individuals. No differences in the number of gene segments defined by probes representing 14 different human V beta subfamilies and the constant region genes were found. The distribution of haplotypes defined by restriction fragment length polymorphism (RFLP) alleles detected with V beta 8, V beta 11, and C beta probes in the MS patients was significantly different from that found in normal individuals. Because 84% of the MS patients were DR2+, the findings in these patients were compared to a second group of 43 normals who were DR2+. The distribution of TcR haplotypes in MS patients was also significantly different from that in the DR2+ normals. The data suggest that an MS susceptibility gene(s) may be located in the region of the TcR beta-chain gene complex.
Beall
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SS
Division of Biology, California Institute of Technology, Pasadena 91125.
Concannon
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P
Charmley
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P
McFarland
H F
HF
Gatti
R A
RA
Hood
L E
LE
McFarlin
D E
DE
Biddison
W E
WE
D000483:Alleles; D004198:Disease Susceptibility; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D006684:HLA-DR Antigens / Q000032:analysis; D015802:HLA-DR2 Antigen; D006801:Humans; D009103:Multiple Sclerosis / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D016693:Receptors, Antigen, T-Cell, alpha-beta; D013601:T-Lymphocytes / Q000032:analysis
D006684:HLA-DR Antigens; D015802:HLA-DR2 Antigen; D011948:Receptors, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta