[Nuclear DNA analysis of hepatocellular carcinoma].
Retrospective DNA ploidy studies of paraffin-embedded blocks were performed by flow cytometry on 212 surgically resected hepatocellular carcinoma. One hundred and sixty-four of 212 specimens yielded evaluable DNA histograms. Eighty-three cases showed a DNA diploid pattern and 81 a DNA non-diploid pattern. The incidence of non-diploid pattern increased with the tumor size. (2cm; 15.4%, 2-5; 42.3%, 5-10; 70.8% greater than 10; 73.3%). The DNA pattern correlated with the backgrounds, the degree of vascular invasion, intrahepatic metastasis and serum AFP levels. Survival of patients with non-diploid pattern was significantly less than those with diploid patients in any different stage. Of thirty who survived three years with disease free condition, 20 patients showed diploid patterns. Though remaining 10 patients were non-diploid patterns, 9 were high DNA index more than 1.5. It is concluded that flow cytometric DNA ploidy study had prognostic value for patients with surgically resected hepatocellular carcinoma.
Fujimoto
J
J
First Department of Surgery, Hyogo College of Medicine, Nishinomiya, Japan.
Okamoto
E
E
Yamanaka
N
N
Fujiwara
S
S
Kato
T
T
Mitsunobu
M
M
Toyosaka
A
A
D006528:Carcinoma, Hepatocellular / Q000032:analysis*; D002467:Cell Nucleus / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry; D006801:Humans; D008113:Liver Neoplasms / Q000032:analysis*; D011003:Ploidies; D011379:Prognosis
D004273:DNA, Neoplasm