title: Rate enhancement of the electron transfer the adrenodoxin-adrenodoxin reductase system by dicarboxylic acids.
abstract: The rate of electron transport in the cytochrome P-450 system in adrenocortical mitochondria was studied with purified adrenodoxin reductase, adrenodoxin and cytochrome c. Oxaloacetate enhanced the rate at concentrations of less than 1 mM; malate, succinate and fumarate enhanced the rate to a lesser extent; and pyruvate and alpha-ketoglutarate had no appreciable effect. The rate enhancement was observed when the reagents were preincubated with adrenodoxin, but not with adrenodoxin reductase. Rate enhancement was also evident when the rate limiting step was at adrenodoxin in the electron transport system.
authors.affiliation: Department of Biochemistry, Osaka University Medical School, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yamano
authors.forename: Y
authors.initials: Y
authors.lastname: Nonaka
authors.forename: M
authors.initials: M
authors.lastname: Okamoto
authors.forename: T
authors.initials: T
authors.lastname: Matsubara
authors.forename: R
authors.initials: R
authors.lastname: Miura
mesh_terms: D000325:Adrenodoxin / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D003574:Cytochrome c Group / Q000378:metabolism; D003998:Dicarboxylic Acids / Q000494:pharmacology*; D004579:Electron Transport / Q000187:drug effects; D005287:Ferredoxin-NADP Reductase / Q000378:metabolism* / Q000502:physiology; D007700:Kinetics; D008928:Mitochondria / Q000201:enzymology; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D010071:Oxaloacetates / Q000494:pharmacology; D010084:Oxidation-Reduction
chemical_list: D003574:Cytochrome c Group; D003998:Dicarboxylic Acids; D010071:Oxaloacetates; D000325:Adrenodoxin; D005287:Ferredoxin-NADP Reductase; D009247:NADH, NADPH Oxidoreductases