Cortisol-stimulated phosphoinositide metabolism in vascular smooth muscle cells: a role for glucocorticoids in blood pressure control?
Physiological concentrations of cortisol (0.02-5.0 micrograms/ml) stimulate the phosphoinositide breakdown in cultured smooth muscle cells from rat aorta. After a 15-min stimulation of the cells with various concentrations of cortisol, the inositol-1,4,5-triphosphate concentrations showed a dose-dependent increase. This phenomenon was qualitatively similar but quantitatively more sensitive in smooth muscle cells from spontaneously hypertensive rats (SHR) than in vascular smooth muscle cells from normotensive animals. When the antihormone RU 486 was added before the vascular smooth muscle cells were stimulated with cortisol, the inositol-1,4,5-triphosphate production was reduced to 50%.
Steiner
A
A
Department of Internal Medicine, University Hospital, Zürich, Switzerland.
Locher
R
R
Sachinidis
A
A
Vetter
W
W
D000818:Animals; D001794:Blood Pressure / Q000502:physiology*; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005938:Glucocorticoids / Q000502:physiology*; D006854:Hydrocortisone / Q000494:pharmacology*; D015735:Mifepristone / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000187:drug effects* / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D013268:Stimulation, Chemical
D005938:Glucocorticoids; D010716:Phosphatidylinositols; D015735:Mifepristone; D011493:Protein Kinase C; D006854:Hydrocortisone