title: Presence and release of calcitonin gene-related peptide in rat stomach.
abstract: Immunoreactive (IR)-calcitonin gene-related peptide (CGRP) was identified throughout the entire stomach of rats, being most highly concentrated in the pyloric region, and the concentrations in muscular layers being higher than those in mucosal layers. In addition, IR-CGRP was also present in the venous effluent from isolated perfused rat stomach, and its release was stimulated by dibutyryl cyclic AMP or theophylline but not by glucagon. Gel chromatography as well as HPLC of both tissue extracts and gastric perfusate showed three identical major peaks of IR-CGRP, one of which coeluted with synthetic CGRP. These results suggest that CGRP in the stomach plays a role in the regulation of gastric function.
authors.affiliation: Department of Medicine, Kobe University School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Inui
authors.forename: T
authors.initials: T
authors.lastname: Chiba
authors.forename: Y
authors.initials: Y
authors.lastname: Okimura
authors.forename: T
authors.initials: T
authors.lastname: Morishita
authors.forename: A
authors.initials: A
authors.lastname: Nakamura
authors.forename: A
authors.initials: A
authors.lastname: Yamaguchi
authors.forename: T
authors.initials: T
authors.lastname: Yamatani
authors.forename: S
authors.initials: S
authors.lastname: Kadowaki
authors.forename: K
authors.initials: K
authors.lastname: Chihara
authors.forename: T
authors.initials: T
authors.lastname: Fujita
mesh_terms: D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis / Q000378:metabolism*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005753:Gastric Mucosa / Q000378:metabolism*; D005934:Glucagon / Q000494:pharmacology; D008297:Male; D011708:Pylorus / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D013270:Stomach / Q000032:analysis / Q000187:drug effects; D013806:Theophylline / Q000494:pharmacology; D014018:Tissue Distribution
chemical_list: D003994:Bucladesine; D005934:Glucagon; D013806:Theophylline; D015740:Calcitonin Gene-Related Peptide