[Development of ACTH-containing cells in the hypothalamus and hypophysis in their normal state and in protein deficiency].
In cryostate sections of the brain and hypophysis of newborn, 10- and 21-day-old rats, by means of the indirect method immunohistochemical reaction has been performed. As a primary serum rabbit antiserum against ACTH has been used. The ventromedial hypothalamus and adenohypophysis have been studied by means of light and electron microscopy. Relationship of ACTH-containing cells in the adenohypophysis in the process of the postnatal ontogenesis and protein insufficiency remains constant. Unlike the adenohypophysis, in the hypothalamus part of the ACTH-containing cells decreases with age. When the animals develop under conditions of protein insufficiency, changes in the part of the ACTH-positive cells, that are age-dependent, do not coincide with corresponding parameters in the control. In the adenohypophyseal cells the products of diaminobensidine reaction are distributed in cytoplasm of granules with a dense center 100-200 nm in the diameter. The ACTH-containing cells in the hypothalamus are presented as small neurons with an even distribution of the immunohistochemical reaction products in cytoplasm and in large granules 200-280 nm in the diameter. Since in the adenohypophysis and in the hypothalamus various dynamics on contents of ACTH-reactive cells in the postnatal ontogenesis and at protein insufficiency has been revealed, and various localization of the diaminobenzidine reaction products has been demonstrated, a conclusion is made on heterogenous nature of antigenic determinants against ACTH, situating in these structures.
Babichenko
I I
II
Savrova
O B
OB
D000324:Adrenocorticotropic Hormone / Q000096:biosynthesis*; D000367:Age Factors; D000818:Animals; D007031:Hypothalamus / Q000166:cytology* / Q000473:pathology; D008854:Microscopy, Electron; D009474:Neurons / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D010903:Pituitary Gland, Anterior / Q000166:cytology* / Q000473:pathology; D011502:Protein-Energy Malnutrition / Q000473:pathology*; D051381:Rats; D011919:Rats, Inbred Strains
D000324:Adrenocorticotropic Hormone
Formirovanie AKTG-soderzhashchikh kletok gipotalamusa i gipofiza v norme i pri belkovoĭ nedostatochnosti.