Rapid identification and typing of herpes simplex virus by a new enzyme immunoassay with peroxidase-labeled complement C1q.
A new enzyme-linked immunoassay system (TC-ELISA) was developed for the rapid, specific detection and typing of herpes simplex virus (HSV) using anti-HSV immune sera and enzyme-labeled complement component C1q (P*-C1q). The method is based on viral antigen produced in HSV-infected cells being detected by simultaneous addition of immune serum and P*-C1q (ELISA-CF). With this assay, it was found that HSV was detected with anti-HSV immune serum and P*-C1q and that HSV type 1 and HSV type 2 could be differentiated with anti-HSV-1 and anti-HSV-2 immune sera and P*-C1q. The TC-ELISA test was HSV specific and not reactive with measles virus, cytomegalovirus, mumps virus or varicella-zoster virus. The whole assay procedure can be completed within 3 hours, as long as HSV-inoculated cell cultures with more than 10% cytopathic effect are used. These results suggest that TC-ELISA is useful in the diagnosis of viral infections.
Lee
S M
SM
Department of Microbiology, School of Hygienic Sciences, Kitasato University, Kanagawa, Japan.
Taguchi
F
F
D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis / Q000096:biosynthesis; D002460:Cell Line; D015922:Complement C1q / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007124:Immunoenzyme Techniques; D012703:Serotyping / Q000379:methods*; D018139:Simplexvirus / Q000276:immunology / Q000302:isolation & purification*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D015922:Complement C1q