Location of signal sequences for membrane insertion of the Na+,K+-ATPase alpha subunit.
To study the membrane insertion of the Na+,K+-ATPase (EC 3.6.1.37) alpha subunit with six to eight transmembrane segments, mRNAs encoding the entire alpha subunit and its four different domains were prepared and translated in rabbit reticulocyte lysate with rough microsomal membranes. On the basis of the resistance of the membrane-inserted products to alkali extraction and the failure to insert the translation products into N-ethylmaleimide-treated membranes, it is suggested that at least two signal sequences are contained within the four transmembrane segments from the amino terminus of the alpha subunit.
Homareda
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Department of Biochemistry, Kyorin University School of Medicine, Tokyo, Japan.
Kawakami
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Nagano
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Matsui
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D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology*; D005033:Ethylmaleimide / Q000494:pharmacology; D015723:Gene Library; D006367:HeLa Cells / Q000201:enzymology; D006801:Humans; D046911:Macromolecular Substances; D010957:Plasmids; D014176:Protein Biosynthesis; D021382:Protein Sorting Signals / Q000235:genetics*; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D015183:Restriction Mapping; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics*; D013698:Templates, Genetic; D014158:Transcription, Genetic
D046911:Macromolecular Substances; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D000254:Sodium-Potassium-Exchanging ATPase; D005033:Ethylmaleimide