Effect of some penicillins on the sensitivity of limulus amoebocyte lysate test.
A group of penicillins have been tested for their effect on the sensitivity of limulus amebocyte lysate test (LAL). Cloxacillin, cephalothin and cefuroxime inhibited the gel formation at concentrations above 2 mg mL-1, while ampicillin, methicillin and mecillinam showed no inhibitory effects upto concentrations of 10 mg mL-1. For benzylepenicillin the maximum non-inhibitory concentration was 10,000 units mL-1. However, the dilutions required to overcome inhibition were within the limits of maximum valid dilutions computed for each product showing that LAL test is valid for these products at their non-inhibitory concentrations.
al-Khalifa
I J
IJ
Drug Control and Registration Centre, Ministry of Health, Safat, Kuwait.
Naidu
N V
NV
Nallari
V R
VR
Raut
H I
HI
D000818:Animals; D004731:Endotoxins / Q000032:analysis; D008033:Limulus Test; D010406:Penicillins / Q000494:pharmacology*; D011817:Rabbits
D004731:Endotoxins; D010406:Penicillins