title: Action potentials of cultured human oat cells: whole-cell measurements with the patch-clamp technique.
abstract: Oat cells (of the small cell carcinoma of the lung) have been reported to generate calcium action potentials. The calcium channels have further been suggested to play a crucial role in the relation between oat cell carcinoma and the often associated myasthenic syndrome. We have examined cultured human oat cells (U-1690) under voltage-clamp conditions, using the patch-clamp technique. We found, contrary to previous reports, that the action potential was caused by sodium and potassium currents. No calcium current was detected under these conditions, which indicates that calcium channels, if present, are very rare. The findings restrict, but do not rule out, the hypothesis that calcium plays a key role in the carcinoma/myasthenic syndrome relation.
authors.affiliation: Nobel Institute for Neurophysiology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: S
authors.initials: S
authors.lastname: Johansson
authors.forename: B
authors.initials: B
authors.lastname: Rydqvist
authors.forename: C
authors.initials: C
authors.lastname: Swerup
authors.forename: E
authors.initials: E
authors.lastname: Heilbronn
authors.forename: P
authors.initials: P
authors.lastname: Arhem
mesh_terms: D000200:Action Potentials; D001984:Bronchial Neoplasms / Q000209:etiology; D015220:Calcium Channels / Q000502:physiology*; D018288:Carcinoma, Small Cell / Q000150:complications / Q000473:pathology / Q000503:physiopathology*; D002478:Cells, Cultured; D003584:Cytological Techniques; D006801:Humans; D008175:Lung Neoplasms / Q000150:complications / Q000473:pathology / Q000503:physiopathology*; D009135:Muscular Diseases / Q000209:etiology; D015221:Potassium Channels / Q000502:physiology; D015222:Sodium Channels / Q000502:physiology; D013577:Syndrome
chemical_list: D015220:Calcium Channels; D015221:Potassium Channels; D015222:Sodium Channels