[Features of temperature dependence of the Na+,K+-ATPase reaction in normal and tumorous brain tissue].
It has been shown that in the enzyme preparations (EP) from normal brain tissue (NBT) a typical break on the Arrhenius plot appeared at 20-22 degrees C, nH for Na+ and K+ exceeding 1.7 and 1.4, respectively. In EP from tumoural brain tissue (TBT) no break on the Arrhenius plot at 20-22 degrees C was revealed, but it appeared at 27.5 + 30.5 degrees C. The nH for Na+ with Na+,K+-ATPase from TBT was only 0.9, but the cooperative binding of K+ was preserved (nH = 1.3). Electrophoregrams (EP) from TBT showed additional protein bands. The urea and digitonin treatment of EP from NBT induced a break on the Arrhenius plot at 27.5-30.5 degrees C. It is suggested that the break at 27.5-30.5 degrees C is, probably, accompanied by local changes in the conformation of protein components of the enzyme.
Salum
T T
TT
Tsil'mer
K
K
Vikhalemm
T E
TE
Kullisaar
T E
TE
Tiakhepyld
L Ia
LIa
Randvere
T O
TO
Sinisalu
V N
VN
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology*; D001932:Brain Neoplasms / Q000201:enzymology* / Q000378:metabolism; D004072:Digitonin / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D011487:Protein Conformation; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013696:Temperature; D014508:Urea / Q000494:pharmacology
D014508:Urea; D000254:Sodium-Potassium-Exchanging ATPase; D004072:Digitonin
Osobennosti temperaturnoĭ zavisimosti Na+,K+-ATP-aznoĭ reaktsii v normal'noĭ i opukholevoĭ tkani mozga.