Pancreastatin: a novel peptide inhibitor of parietal cell signal transduction.
The direct inhibition of secretion by pancreastatin was investigated in rabbit isolated parietal cells. Pancreastatin exerted no influence on basal aminopyrine uptake. Pancreastatin inhibited histamine stimulated aminopyrine uptake through a decrease in intracellular cAMP. Pancreastatin inhibition of histamine stimulated uptake was blocked in the presence of pertussis toxin. Pancreastatin also inhibited the carbachol stimulated increase in aminopyrine accumulation. However, the effects of pancreastatin on carbachol stimulation were not reversed by pertussis toxin. Pancreastatin did not alter the carbachol induced increase in cytosolic free calcium. Thus, pancreastatin appears to inhibit parietal cell signal transduction at multiple points along the second messenger pathways.
Lewis
J J
JJ
Department of Surgery, Yale University School of Medicine, New Haven, CT 06510.
Goldenring
J R
JR
Asher
V A
VA
Modlin
I M
IM
D000632:Aminopyrine / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000032:analysis; D002217:Carbachol / Q000494:pharmacology; D053379:Chromogranin A; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000037:antagonists & inhibitors / Q000378:metabolism; D006632:Histamine / Q000378:metabolism; D066298:In Vitro Techniques; D010187:Pancreatic Hormones / Q000494:pharmacology*; D010295:Parietal Cells, Gastric / Q000187:drug effects* / Q000201:enzymology; D037342:Pertussis Toxin; D011817:Rabbits; D015398:Signal Transduction / Q000187:drug effects*; D013050:Spectrometry, Fluorescence; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D053379:Chromogranin A; D010187:Pancreatic Hormones; D010566:Virulence Factors, Bordetella; D000632:Aminopyrine; C050836:pancreastatin; D005576:Colforsin; D006632:Histamine; D002217:Carbachol; D000242:Cyclic AMP; D037342:Pertussis Toxin; D002118:Calcium