A role for herpes simplex virus type 1 glycoprotein E in induction of cell fusion.
The role of herpes simplex virus type 1 (HSV-1) glycoprotein E (gE) in the induction of multinucleate cell (syncytium) formation was investigated using monoclonal antibodies and a gE deletion mutant, R7023. We found that monoclonal antibodies directed against gE blocked HSV-1-induced syncytium formation in human cells. R7023 also failed to induce syncytium formation in tissue culture cells. The results indicate that gE, in addition to glycoproteins B, D, H and the gene sequence located between 0.732 and 0.745 map units, is involved in cell fusion. Thus, this important biological property appears to be regulated by several HSV-1 gene products.
Chatterjee
S
S
Department of Pediatrics, University of Alabama, Birmingham 35294.
Koga
J
J
Whitley
R J
RJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology; D001667:Binding, Competitive; D002459:Cell Fusion; D002460:Cell Line; D002522:Chlorocebus aethiops; D005347:Fibroblasts / Q000382:microbiology / Q000502:physiology; D006801:Humans; D007668:Kidney; D018139:Simplexvirus / Q000502:physiology*; D014759:Viral Envelope Proteins / Q000276:immunology / Q000502:physiology*
D000911:Antibodies, Monoclonal; D014759:Viral Envelope Proteins; C044768:glycoprotein E, herpes simplex virus type 1