title: Donor blood frozen and stored between -20 degrees C and -25 degrees C with 35-day post-thaw shelf life.
abstract: With standard blood bank facilities and interconnected plastic bags donor erythrocytes can be frozen and stored in commercial freezers at temperatures of -20 degrees to -25 degrees C for at least 6 months. The cryoprotectant, a mixture of glycerol and dextrose, is washed off in a closed-circuit system. After being thawed and reconstituted with an additive solution, the pack of red cells can be stored in liquid form for up to 35 days at 4 degrees-6 degrees C. The system lends itself to routine clinical blood transfusion practice.
authors.affiliation: New South Wales Red Cross Blood Transfusion Service, Sydney, Australia.
authors.forename: V A
authors.initials: VA
authors.lastname: Lovric
authors.forename: D B
authors.initials: DB
authors.lastname: Klarkowski
mesh_terms: D001782:Blood Donors; D001793:Blood Preservation / Q000379:methods*; D001803:Blood Transfusion; D002470:Cell Survival; D002860:Chromium Radioisotopes; D004338:Drug Combinations; D004905:Erythrocyte Aging / Q000187:drug effects*; D004912:Erythrocytes; D005615:Freezing; D005947:Glucose / Q000008:administration & dosage / Q000494:pharmacology; D005990:Glycerol / Q000008:administration & dosage / Q000494:pharmacology; D006801:Humans; D013696:Temperature; D013997:Time Factors
chemical_list: D002860:Chromium Radioisotopes; D004338:Drug Combinations; D005947:Glucose; D005990:Glycerol