Mitogenic properties of two distinct forms of toxic shock syndrome toxin-1 separated on hydroxyapatite by high-performance liquid chromatography.
The homogeneity of a purified staphylococcal toxic shock syndrome toxin-1 (TSST-1) was tested by high-performance methods. This preparation was homogenous in ion-exchange chromatography and isoelectric focusing (pI = 7.4), but was resolved into two distinct peaks by high-performance hydroxyapatite chromatography. Both components, TSST-1hA and TSST-1hB had similar molecular weights (22 kD) and amino acid compositions. TSST-1 did not dimerize or polymerize upon heating at 60 degrees C for 30 min or in solutions with pH varying from 4.0 to 8.5. TSST-1hA and TSST-1hB showed similar immunological reactivity to native TSST-1 goat polyclonal antibodies. TSST-1hA and TSST-1hB as well as staphylococcal enterotoxin A and staphylococcal exfoliative toxin were potent mitogens in lymphocyte proliferation assays. The lymphocyte proliferative response to 10 pg of TSST-1hB was comparable to a response elicited by 10 ng of TSST-1hA, suggesting that the former component is a more potent mitogen. Rabbit or goat polyclonal antibodies to native TSST-1 efficiently neutralized both TSST-1 components. Heat treatment at 80 degrees C for 15 min had minimal or no effect on the mitogenic properties of TSST-1hA and TSST-1hB.
Naidu
A S
AS
Department of Medical Microbiology, University of Lund, Malmö General Hospital, Sweden.
Eriksson
K O
KO
Hallberg
T
T
Lindeberg
J
J
Liao
J L
JL
Yao
K
K
Wadström
T
T
Hjertén
S
S
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001427:Bacterial Toxins; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D017886:Durapatite; D004768:Enterotoxins / Q000032:analysis / Q000276:immunology / Q000494:pharmacology*; D006882:Hydroxyapatites; D007525:Isoelectric Focusing; D008213:Lymphocyte Activation; D008934:Mitogens; D018089:Superantigens
D000596:Amino Acids; D001427:Bacterial Toxins; D004768:Enterotoxins; D006882:Hydroxyapatites; D008934:Mitogens; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal; D017886:Durapatite