title: Hamster liver cholinephosphotransferase and ethanolaminephosphotransferase are separate enzymes.
abstract: CDP-choline:1,2-diacylglycerol cholinephosphotransferase (EC 2.7.8.2) and CDP-ethanolamine:1,2-diacylglycerol ethanolaminephosphotransferase (EC 2.7.8.1) are microsomal enzymes that catalyze the final steps in the syntheses of phosphatidylcholine and phosphatidylethanolamine via the CDP-choline and CDP-ethanolamine pathways, respectively. Both enzyme activities were cosolubilized from hamster liver microsomes by Triton QS-15. Limited separation of these two activities was achieved by ion-exchange chromatography. The partially purified phosphotransferases displayed a higher sensitivity than microsomal phosphotransferases towards exogenous phospholipids and showed an absolute requirement for divalent cations. Upon purification, cholinephosphotransferase was more stable to heat treatment than ethanolaminephosphotransferase. The two enzymes exhibited distinct pH optima and responded differently to exogenous phospholipids. Our results clearly indicate that cholinephosphotransferase and ethanolaminephosphotransferase are separate enzymes.
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Manitoba, Winnipeg.
authors.forename: K M
authors.initials: KM
authors.lastname: O
authors.forename: Y L
authors.initials: YL
authors.lastname: Siow
authors.forename: P C
authors.initials: PC
authors.lastname: Choy
mesh_terms: D000818:Animals; D006224:Cricetinae; D003902:Detergents; D002798:Diacylglycerol Cholinephosphotransferase / Q000302:isolation & purification* / Q000378:metabolism; D004982:Ethanolaminephosphotransferase / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008647:Mesocricetus; D008862:Microsomes, Liver / Q000201:enzymology*; D010743:Phospholipids; D010770:Phosphotransferases / Q000302:isolation & purification*; D012995:Solubility
chemical_list: D003902:Detergents; D010743:Phospholipids; D010770:Phosphotransferases; D004982:Ethanolaminephosphotransferase; D002798:Diacylglycerol Cholinephosphotransferase