Isolation and characterization of new mammalian kinase genes by cross hybridization with a tyrosine kinase probe.
We isolated two novel mammalian kinase genes by weak cross-hybridization with v-ros oncogene. (1) A cDNA of about 7.7 kb obtained from a human placenta cDNA library carried a 4.2 kb open reading frame, and the predicted amino acid sequence of 1338 residues contained extracellular, transmembrane and tyrosine kinase domains. The overall structure including cysteine motifs in its extracellular domain and a long peptide insertion in its tyrosine kinase domain indicates that this new gene is closely related to the fms family. Consequently, the gene was designated as flt(fms-like tyrosine kinase) gene. The expression of the flt gene was examined in normal and transformed cells, and it was mapped to human chromosome 13q12-13. (2) Another new gene was found to be expressed almost exclusively in testis. The cDNA sequence analysis revealed that the predicted product carried protein kinase consensus motifs in its amino-terminal region. Comparison of the deduced amino acid sequence of this gene in the kinase domain with those of other protein kinase genes will be discussed.
Shibuya
M
M
Department of Genetics, University of Tokyo, Japan.
Matsushime
H
H
Yamane
A
A
Ikeda
T
T
Yoshida
M C
MC
Tojo
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002882:Chromosomes, Human, Pair 13; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D005796:Genes; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D015394:Molecular Structure; D005810:Multigene Family; D009856:Oncogene Proteins, Viral / Q000235:genetics; D009857:Oncogenes; D016366:Open Reading Frames; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D051381:Rats; D020794:Receptor Protein-Tyrosine Kinases; D011956:Receptors, Cell Surface / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013737:Testis / Q000201:enzymology; D040281:Vascular Endothelial Growth Factor Receptor-1
D015342:DNA Probes; D009856:Oncogene Proteins, Viral; D011518:Proto-Oncogene Proteins; D011956:Receptors, Cell Surface; D004247:DNA; D011505:Protein-Tyrosine Kinases; D020794:Receptor Protein-Tyrosine Kinases; D040281:Vascular Endothelial Growth Factor Receptor-1; C058682:v-ros protein, Avian sarcoma virus UR2