Identification of B2 bradykinin binding sites in the guinea pig nasal turbinate.
Specific high affinity BK binding sites in the nasal turbinate of the guinea pig have been demonstrated. Specific [3H]BK binding (10-330 pM) was saturable, and nonlinear least squares analysis indicated the presence of a high affinity binding site with a Kd value of 60 (50-78) pM and a Bmax value of 13.1 = 2.0 fmol/mg protein. In inhibition experiments, D-Phe7-BK (a B2 antagonist) inhibited [3H]BK binding with a Ki value of 23 nM, while des-Arg9[Leu8]-BK (a B1 antagonist) had no effect up to a concentration of 10 microM. These studies indicate the presence of B2 BK receptors in the guinea pig nasal turbinate.
Fujiwara
Y
Y
Department of Pharmacology, University of Arizona Health Sciences Center, Tucson 85724.
Bloom
J W
JW
Mantione
C R
CR
Yamamura
H I
HI
D000818:Animals; D001667:Binding, Competitive; D001920:Bradykinin / Q000378:metabolism*; D006168:Guinea Pigs; D007700:Kinetics; D008297:Male; D018002:Receptors, Bradykinin; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D014420:Turbinates / Q000378:metabolism*
D018002:Receptors, Bradykinin; D017981:Receptors, Neurotransmitter; D001920:Bradykinin