Platelet-derived-growth-factor-stimulated heterogeneous polyphosphoinositide metabolism and phosphate uptake in C3H fibroblasts.
Pig platelet-derived growth factor (PDGF) increased the rate of [32P]Pi uptake by murine fibroblasts, resulting in a 3-9-fold elevation of the specific radioactivity of ATP, PtdInsP, PtdInsP2, PtdIns and phosphatidic acid. The specific radioactivity was 10-60-fold higher in ATP than in the four phospholipids. These substances are therefore not in metabolic equilibrium, which complicates determination of inositol phospholipid turnover.
Holmsen
H
H
Department of Biochemistry, University of Bergen, Norway.
Male
R
R
Rongved
S
S
Langeland
N
N
Lillehaug
J
J
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D010710:Phosphates / Q000378:metabolism*; D010712:Phosphatidic Acids / Q000378:metabolism; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols / Q000378:metabolism*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*
D010710:Phosphates; D010712:Phosphatidic Acids; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols; D010982:Platelet-Derived Growth Factor; D000255:Adenosine Triphosphate