Genes downstream from pucB and pucA are essential for formation of the B800-850 complex of Rhodobacter capsulatus.
The formation of the light-harvesting complex B800-850 (LH-II) of Rhodobacter capsulatus requires, in addition to the synthesis of the polypeptides alpha and beta (the gene products of pucA and pucB), the synthesis of bacteriochlorophyll and carotenoids and the expression of at least one gene localized downstream from the pucBA operon. This was concluded from the observation that a Tn5 insertion downstream from pucBA inhibited the formation of the LH-II complex and the formation of the pucBA mRNA. The Tn5 insertion point was mapped and found to be over 500 base pairs (bp) downstream from the end of the pucA gene, suggesting the presence of additional puc genes. A region of about 3,000 bp including the pucB and pucA genes and DNA downstream from pucA was sequenced and found to contain three open reading frames (ORFs C, D, and E). The polypeptide deduced from the first ORF (C) contains 403 amino acids with strongly hydrophobic stretches and one large and three small hydrophilic domains carrying many charged residues. The other two ORFs contain 113 (D) and 118 (E) codons. The amino acid sequences of the N terminus and two tryptic peptides of an alkaline-soluble Mr-14,000 subunit of the isolated LH-II complex were identical with the deduced amino acid sequence of ORF E.
Tichy
H V
HV
Institut für Biologie II, Universität Freiburg, Federal Republic of Germany.
Oberlé
B
B
Stiehle
H
H
Schiltz
E
E
Drews
G
G
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D045342:Light-Harvesting Protein Complexes; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D009876:Operon; D045322:Photosynthetic Reaction Center Complex Proteins; D010957:Plasmids; D011487:Protein Conformation; D015183:Restriction Mapping; D012241:Rhodopseudomonas / Q000235:genetics*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D045342:Light-Harvesting Protein Complexes; D015345:Oligonucleotide Probes; D045322:Photosynthetic Reaction Center Complex Proteins