title: An approach to study the evolution of the Drosophila 5S ribosomal genes using P-element transformation.
abstract: The P-element-mediated gene transfer system was used to introduce Drosophila teissieri 5S genes into the Drosophila melanogaster genome. Eight transformed D. melanogaster strains that carry D. teissieri 5S mini-clusters consisting of 9-21 adjacent 5S units were characterized. No genetic exchanges between D. melanogaster and D. teissieri 5S clusters were detected over a 2-year survey of the eight strains. The occurrence of small rearrangements within the D. melanogaster 5S cluster was demonstrated in one of the transformed strains.
authors.affiliation: Laboratoire d'Embryologie Moléculaire et Expérimentale, Centre National de la Recherche Scientifique, Université Paris 11, Orsay, France.
authors.forename: M L
authors.initials: ML
authors.lastname: Samson
authors.forename: M
authors.initials: M
authors.lastname: Wegnez
mesh_terms: D000818:Animals; D004251:DNA Transposable Elements; D004330:Drosophila / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics; D015321:Gene Rearrangement; D005821:Genetic Techniques; D005810:Multigene Family; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D014170:Transformation, Genetic
chemical_list: D004251:DNA Transposable Elements; D012335:RNA, Ribosomal; D012341:RNA, Ribosomal, 5S