Further characterisation of the dopamine-inhibitory receptor in Helix and evidence for a noradrenaline-preferring receptor.
1. The cells in this study responded with a hyperpolarization to the following agents in this order of potency; dopamine greater than noradrenaline phenylephrine = octopamine. 2. 6,7 ADTN had a relative potency of 0.1 compared to dopamine. 5,6 ADTN did not inhibit the cells in this study. 3. The D1 receptor agonists SKF38393 and dihydroxynomifensine mimicked the effect of dopamine on these cells but were over 100 times less active, whereas the D2 selective agonists quinpirole and RU24213 were without effect. 4. Both the D1 antagonist SCH23390 and the D2 antagonist sulpiride antagonised the dopamine response with pA2 values of 6.1 and 6.7, respectively. 5. Five cells that responded to dopamine with a hyperpolarization were depolarized by noradrenaline. The order of potency of compounds at eliciting this depolarization, noradrenaline greater than phenylephrine greater than octopamine indicated that this response may be mediated by a noradrenaline-preferring receptor.
Holden-Dye
L
L
Department of Neurophysiology, University of Southampton, U.K.
Walker
R J
RJ
D000818:Animals; D006372:Helix, Snails / Q000378:metabolism*; D008564:Membrane Potentials / Q000187:drug effects; D009638:Norepinephrine / Q000494:pharmacology*; D011941:Receptors, Adrenergic / Q000032:analysis*; D011954:Receptors, Dopamine / Q000032:analysis* / Q000187:drug effects
D011941:Receptors, Adrenergic; D011954:Receptors, Dopamine; D009638:Norepinephrine