Comparative metabolism of benzo[f]quinoline by liver microsomes from brown bullheads and rats.
1. Liver microsomes from rats were considerably more active in metabolizing benzo[f]quinoline (B f Q) than those from brown bullheads (Ictalurus nebulosus). 2. The main B f Q metabolites formed by both rat and brown bullhead liver microsomes were qualitatively similar and included B f Q-7,8-dihydrodiol, B f Q-9,10-dihydrodiol, B f Q-N-oxide, 7-hydroxy B f Q, and 9-hydroxy B f Q. 3. The liver microsomes from control brown bullheads and rats metabolized B f Q primarily at the 7,8-and 9,10-positions, respectively, whereas in the case of microsomes from 3-methylcholanthrene (3-MC)-treated rats or brown bullheads, the major site of metabolic attack was the 7,8-position. 4. A 3-MC-type of cytochrome P-450 appears to be primarily responsible for the oxidation of B f Q by control brown bullhead liver microsomes, whereas a phenobarbital-inducible type of cytochrome P-450 seems to be involved in the metabolism of B f Q by control rat liver microsomes.
Kandaswami
C
C
Great Lakes Laboratory, State University of New York College, Buffalo 14222.
Rutkowski
J P
JP
Kumar
S
S
Sikka
H C
HC
D000818:Animals; D002397:Catfishes / Q000378:metabolism*; D007059:Ictaluridae / Q000378:metabolism*; D008862:Microsomes, Liver / Q000378:metabolism*; D009153:Mutagens / Q000378:metabolism*; D011804:Quinolines / Q000378:metabolism*; D051381:Rats / Q000378:metabolism*
D009153:Mutagens; D011804:Quinolines; C039108:benzo(f)quinoline