Inhibition by triphenyltin chloride of cytosolic free calcium mobilization in human neutrophils stimulated by a chemotactic factor.
We examined the effects of triphenyltin chloride (TPTCl) on the signal transduction in cells, especially the rise of cytosolic free calcium in fura-2-loaded neutrophils stimulated by N-folmyl-L-methionyl-L-leucyl-L-phenylalanine (FMLP). The peak concentration of cytosolic free calcium in neutrophils stimulated by FMLP (10(-7) M) after treatment with 4 mM EGTA was inhibited by TPTCl in a dose-dependent manner and completely blocked in the concentration range of 2.5 to 10 microM in the absence of extracellular calcium. TPTCl also had dose-dependent inhibitory effects on the superoxide anion production and the secretion of beta-glucuronidase in neutrophils stimulated by FMLP (10(-7) M). These results indicate that TPTCl is potent inhibitor of cytosolic free calcium mobilization in FMLP stimulation, associated with superoxide anion production and the secretion of beta-D-glucuronidase.
Miura
Y
Y
Department of Hygiene, Dokkyo University School of Medicine, Tochigi, Japan.
Matsui
H
H
D000328:Adult; D002118:Calcium / Q000378:metabolism*; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism*; D005966:Glucuronidase / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D009947:Organotin Compounds / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism
D009947:Organotin Compounds; D013481:Superoxides; D009240:N-Formylmethionine Leucyl-Phenylalanine; D005966:Glucuronidase; C012594:triphenyltin chloride; D002118:Calcium