title: Production of interleukin 1-like factor from human peripheral blood monocytes and polymorphonuclear leukocytes by superoxide anion: the role of interleukin 1 and reactive oxygen species in inflamed sites.
abstract: In the present study, we investigated the possibility that oxidative stress to human peripheral blood monocytes and polymorphonuclear leukocytes (PMNs) can induce interleukin 1 (IL-1)-like activity. Oxidative stress that we used was superoxide anion (O2-) and hydrogen peroxide (H2O2). O2-, but not H2O2, could induce an IL-1-like factor(s) from monocytes and PMNs. IL-1-like activity from monocytes and PMNs induced by O2- was due to de novo synthesis because no IL-1-like activity was found in culture supernatants and in the lysate of unstimulated cells. We next examined the effects of radical scavengers on production of an IL-1-like factor(s). Generation of IL-1-like activity from monocytes was amplified by preincubation with catalase (H2O2 scavenger), although it was suppressed by preincubation with either superoxide dismutase (O2- scavenger) or vitamin E (antioxidant analogs). These results suggest that production of an IL-1-like factor(s) from monocytes and PMNs was due to O2- stimulation. Our data that production of an IL-1-like factor(s) from inflammatory cells by stimulation with O2- imply a model of the up-regulation mechanism of inflammation mediated by enhanced IL-1-like factor production stimulated with reactive oxygen species.
authors.affiliation: First Department of Internal Medicine, Showa University School of Medicine, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kasama
authors.forename: K
authors.initials: K
authors.lastname: Kobayashi
authors.forename: T
authors.initials: T
authors.lastname: Fukushima
authors.forename: M
authors.initials: M
authors.lastname: Tabata
authors.forename: I
authors.initials: I
authors.lastname: Ohno
authors.forename: M
authors.initials: M
authors.lastname: Negishi
authors.forename: H
authors.initials: H
authors.lastname: Ide
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
authors.forename: Y
authors.initials: Y
authors.lastname: Niwa
mesh_terms: D005949:Glucose Oxidase / Q000502:physiology; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D066298:In Vitro Techniques; D007249:Inflammation / Q000503:physiopathology*; D007375:Interleukin-1 / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000032:analysis; D009000:Monocytes / Q000502:physiology*; D009504:Neutrophils / Q000502:physiology*; D010084:Oxidation-Reduction; D010587:Phagocytosis; D013481:Superoxides / Q000378:metabolism*; D014969:Xanthine Oxidase / Q000502:physiology
chemical_list: D007375:Interleukin-1; D007376:Interleukin-2; D013481:Superoxides; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D014969:Xanthine Oxidase