title: Demonstration by genetic suppression of interaction of GroE products with many proteins.
abstract: The way in which proteins attain and maintain their final form is of fundamental importance. Recent work has focused on the role of a set of ubiquitous proteins, termed chaperonins, in the assembly of phage and multisubunit proteins. The range of chaperonin action is unknown; they could interact with most cellular polypeptides or have a limited subset of protein partners. Included in the chaperonin family is the essential heat-shock regulated Escherichia coli groEL gene product. Over-expression of the groE operon in E. coli causes enhanced assembly of heterologously expressed ribulose bisphosphate carboxylase subunits and suppresses the heat-sensitive mutant phenotype of several dnaA alleles. It has been inferred that suppression of heat-sensitive mutations is confined to dnaA alleles and that this confinement could reflect an interaction between the groE operon products and a dnaA protein aggregate at the replication origin. We now report that multiple copies of the groE operon suppress mutations in genes encoding several diverse proteins. Our data indicate a general role for the groE operon products, the GroEL and GroES proteins, in the folding-assembly pathways of many proteins.
authors.affiliation: Central Research and Development Department, E.I. du Pont de Nemours and Company, Wilmington, Delaware 19880-0328.
authors.forename: T K
authors.initials: TK
authors.lastname: Van Dyk
authors.forename: A A
authors.initials: AA
authors.lastname: Gatenby
authors.forename: R A
authors.initials: RA
authors.lastname: LaRossa
mesh_terms: D000483:Alleles; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D018833:Chaperonins; D003062:Codon / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D009154:Mutation; D009876:Operon; D010641:Phenotype; D010957:Plasmids; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D012483:Salmonella Phages / Q000235:genetics; D012486:Salmonella typhimurium / Q000235:genetics*; D013489:Suppression, Genetic
chemical_list: D001426:Bacterial Proteins; D003062:Codon; D011506:Proteins; D018833:Chaperonins