Construction and characterization of versatile kanamycin-resistance cassettes derived from the Tn5 transposon.
We have developed plasmids with the Tn5 kanamycin-resistance gene (kan) flanked either symmetrically or asymmetrically by several restriction sites. These can be used to provide a selectable genetic marker or to mobilize restriction sites and sense or nonsense codons into genes. The 1.3-kb kan cassette exhibits polarity effects in both directions.
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Department of Biological Sciences, University of South Carolina, Columbia 29208.
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D001483:Base Sequence; D003062:Codon; D004251:DNA Transposable Elements; D007613:Kanamycin Resistance / Q000235:genetics*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D015183:Restriction Mapping
D003062:Codon; D004251:DNA Transposable Elements; D015345:Oligonucleotide Probes