Affinity labeling of ACTH receptors in bovine adrenal cortex membranes.
Adrenocorticotropin labeled with 125I at Tyr23 [(125I-Tyr23]ACTH) was prepared by radioiodination of ACTH1-39 followed by reverse phase HPLC purification. When incubated with bovine adrenal cortical membranes, the radioligand bound specifically to a 40-kDa membrane protein as revealed by affinity labeling. This result indicates that the bovine adrenal ACTH receptor, whose identification and characterization have proved difficult, has an Mr of about 40,000.
Mizuno
T
T
Department of Biological Sciences, Tokyo Institute of Technology, Japan.
Okada
M
M
Itoh
A
A
Maruyama
T
T
Hagiwara
H
H
Hirose
S
S
D000302:Adrenal Cortex / Q000032:analysis*; D000345:Affinity Labels; D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D011956:Receptors, Cell Surface / Q000032:analysis*; D014443:Tyrosine / Q000493:pharmacokinetics
D000345:Affinity Labels; D011956:Receptors, Cell Surface; D014443:Tyrosine