Failure to deduce a peptide inhibitor of Na,K-ATPase from the gene coding for the catalytic alpha-subunit of Na,K-ATPase.
It has been proposed (Bost et al. 1985, Blalock & Bost 1986, Smith et al. 1987) that the DNA strand opposite the one coding, for example, for a hormone or a neurotransmitter codes for its receptor protein. This proposal was tested with regard to the possibility of deducing a peptide inhibitor of Na,K-ATPase from the known gene of the catalytic alpha-subunit of the enzyme. Three such peptides, deduced from presumed extracellular domains of the catalytic alpha-subunit, were tested in vitro and in vivo with regard to their effects on Na,K-ATPase activity. No effect on the enzyme could be demonstrated.
Lundgren
O
O
Department of Physiology, Gothenburg University, Sweden.
Haglind
E
E
Mårdh
S
S
D009597:4-Nitrophenylphosphatase / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D002384:Catalysis; D004247:DNA; D005815:Genetic Code; D008957:Models, Genetic; D010042:Ouabain / Q000008:administration & dosage; D010455:Peptides / Q000008:administration & dosage / Q000235:genetics; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism
D010455:Peptides; D010042:Ouabain; D004247:DNA; D009597:4-Nitrophenylphosphatase; D000254:Sodium-Potassium-Exchanging ATPase