Systemic and mucosal cell mediated immune responses against herpes simplex virus in vaccinated guinea-pigs detected by lymphocyte proliferation and monoclonal antibodies against macrophages and T cells.
Peripheral blood lymphocytes from guinea-pigs immunized with a herpes simplex virus (HSV) sub-unit vaccine (Skinner vaccine) were shown to produce an HSV antigen-specific lymphocyte proliferative response post vaccination. This response was associated with a reduction, compared with mock vaccinated and unvaccinated animals, in the clinical disease scores post intravaginal challenge. This reduction was not associated with the presence of macrophage or T cell infiltration in the vaginal mucosa postchallenge. In contrast mock vaccinated and unvaccinated guinea-pigs did not produce an HSV antigen-specific response post-vaccination but did demonstrate HSV antigen-specific lymphocyte proliferative responses post intra-vaginal challenge. The mock and unvaccinated animals had higher clinical disease scores postchallenge compared with sub-unit vaccinated animals and this was associated with the presence of cellular infiltrates in the vaginal mucosa. These results are discussed in relation to the immunopathology of the disease.
McBride
B W
BW
Division of Pathology, P.H.L.S. Centre for Applied Microbiology and Research, Salisbury, Wiltshire, UK.
Ridgeway
P
P
Phillpotts
R
R
Newell
D G
DG
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D005260:Female; D006168:Guinea Pigs; D006558:Herpes Genitalis / Q000276:immunology* / Q000517:prevention & control; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006825:Hybridomas; D007111:Immunity, Cellular; D007150:Immunohistochemistry; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology; D051379:Mice; D009092:Mucous Membrane / Q000166:cytology; D018139:Simplexvirus / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D014621:Vagina / Q000166:cytology; D014765:Viral Vaccines / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II; D014765:Viral Vaccines