A SepPak HPLC method for tricyclic antidepressant drugs in human vitreous humor.
Death from tricyclic antidepressant overdose has become an all-too-common occurrence. Several factors, including postmortem concentration changes, can render blood and tissue samples useless for the determination of antidepressant drug concentrations. We present here an efficient method of solid-phase extraction for these drugs from vitreous humor and a reversed-phase, isocratic high-performance liquid chromatographic method for the simultaneous quantitation of amitriptyline, doxepin, and imipramine and their desmethylated metabolites.
Evenson
M A
MA
University of Wisconsin, Department of Pathology, Madison 53706.
Engstrand
D A
DA
D000639:Amitriptyline / Q000031:analogs & derivatives / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004316:Doxepin / Q000031:analogs & derivatives / Q000032:analysis*; D006801:Humans; D007099:Imipramine / Q000031:analogs & derivatives / Q000032:analysis*; D012822:Silicon Dioxide; D014822:Vitreous Body / Q000032:analysis*
D004316:Doxepin; D000639:Amitriptyline; D012822:Silicon Dioxide; C042953:octadecyl silane-bonded silica; D007099:Imipramine