Duck hepatitis B virus can tolerate insertion, deletion, and partial frameshift mutation in the distal pre-S region.
In-frame and frameshift mutations were introduced into the pre-S region (1,212 base pairs) of duck hepatitis B virus. The in-frame mutants retained the inserted 12 nucleotides, while the frameshift mutants either reverted to wild type or exhibited a 10-nucleotide compensatory deletion downstream of the original mutation site. Thus, although duck hepatitis B virus has a compact and highly economical genome organization, it can replicate despite alterations of up to 9 amino acid codons in the pre-S and P open reading frames.
Li
J S
JS
Unité de Recherche sur les Hepatites, Institut National de la Santé et de la Recherche Médicale U 271, Lyon, France.
Cova
L
L
Buckland
R
R
Lambert
V
V
Deléage
G
G
Trépo
C
C
D000818:Animals; D001483:Base Sequence; D002872:Chromosome Deletion; D004251:DNA Transposable Elements; D004279:DNA, Viral / Q000235:genetics; D004372:Ducks; D005814:Genes, Viral; D015611:Hepatitis B Virus, Duck / Q000235:genetics*; D008099:Liver / Q000382:microbiology; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids; D015183:Restriction Mapping; D014162:Transfection
D004251:DNA Transposable Elements; D004279:DNA, Viral