The oxygen dependence of the energy state of cardiac tissue: 31P-NMR and optical measurement of myoglobin in perfused rat heart.
The relationship between the energy state and intracellular oxygen concentration was established with cardiac tissue, where the former could be monitored by 31P-NMR and the latter by optical method for myoglobin absorption. The ratio of creatinephosphate to inorganic phosphate, PCr/Pi, started to fall at higher intracellular oxygen concentration than oxygen consumption (10IM). The pH shift measured by frequency shift of Pi paralleled the increase of lactate-release, of which half maximum was approximately 3IM. The critical oxygen concentrations differed nearly 5-fold when determined by energy state and oxygen consumption.
Fukuda
H
H
Department of Cardiovascular Medicine, School of Medicine, Hokkaido University, Sapporo, Japan.
Yasuda
H
H
Shimokawa
S
S
Tamura
M
M
D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D003576:Electron Transport Complex IV / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation / Q000379:methods; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D009211:Myoglobin / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D010100:Oxygen / Q000032:analysis*; D010101:Oxygen Consumption; D010477:Perfusion; D010758:Phosphorus; D051381:Rats
D000227:Adenine Nucleotides; D009211:Myoglobin; C017595:oxymyoglobin; D009243:NAD; D010758:Phosphorus; D003576:Electron Transport Complex IV; D010100:Oxygen