[Dissociation of hexamers of cytochrome P-450 LM2 in the presence of the non-ionic detergent emulgin 913].
It was shown that the maximal degree of dissociation of cytochrome P-450 LM2 hexamers in the presence of the nonionic detergent Emulgene 913 (20 degrees C) is observed at the detergent concentration of about 0.2%. Using equilibrium centrifugation in solutions of different density, the molecular mass of the dissociation product minus that of the bound detergent was found to be equal to 50 +/- 8 kDa, thus corresponding to the molecular mass of the monomer. One cytochrome P-450 LM 2 molecule binds 80 +/- 20 molecules of Emulgene 913.
Berndt
P
P
Magretova
N N
NN
Miasoedova
K N
KN
Cherniak
V Ia
VIa
D002498:Centrifugation; D003577:Cytochrome P-450 Enzyme System; D003902:Detergents; D046911:Macromolecular Substances; D008970:Molecular Weight; D017137:Nonoxynol; D011092:Polyethylene Glycols; D013501:Surface-Active Agents
D003902:Detergents; D046911:Macromolecular Substances; D013501:Surface-Active Agents; D017137:Nonoxynol; D011092:Polyethylene Glycols; D003577:Cytochrome P-450 Enzyme System
Dissotsiatsiia geksamerov tsitokhroma P-450 LM2 v prisutstvii neionnogo detergenta émul'gena 913.