title: Role of pertussigen (pertussis toxin) on the mouse protective activity of vaccines made from Bordetella species.
abstract: Pertussigen [pertussis toxin (Ptx)] from Bordetella pertussis, when detoxified, induces protection in mice to intracerebral challenge (ic) with virulent B. pertussis. In its native form, minute nonprotective doses promote the development of immunity induced by other antigens of B. pertussis. As little as 4 ng of Ptx, given with a nonprotective dose of 8 X 10(7) killed cells of the phase III Sakairi strain, promoted detectable protection to ic challenge. Native Ptx in doses of 0.4 to 400 ng did not protect mice, and vaccines made from strains not producing Ptx induced only weak protection. The marked enhancing action of Ptx was also observed with 5 micrograms of purified filamentous hemagglutinin and with vaccines made from other species of the Bordetella genus, such as B. parapertussis and B. bronchiseptica, but it was not observed with B. pertussis endotoxin. In addition, Ptx was still effective when given as late as 7 days after the vaccine. Antibodies to surface antigens of the challenge strain were demonstrated in sera of mice immunized with vaccines prepared with the different Bordetella species tested, but antibodies to Ptx were detected only in the sera of mice immunized with the wild-type B. pertussis strains. Glutaraldehyde detoxified Ptx does not have this action. Pretreatment of normal mice with Ptx, also enhanced the protective action of a mouse antiserum to a wild-type strain of B. pertussis. These observations show that antigens other than Ptx are responsible for the protection, and that Ptx acts non-specifically to enhance the mouse protective action of those antigens.
authors.affiliation: Department of Health and Human Services, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840.
authors.forename: J J
authors.initials: JJ
authors.lastname: Munoz
authors.forename: M G
authors.initials: MG
authors.lastname: Peacock
mesh_terms: D000276:Adjuvants, Immunologic / Q000276:immunology / Q000494:pharmacology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology; D001885:Bordetella Infections / Q000276:immunology / Q000517:prevention & control; D001886:Bordetella pertussis / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D006388:Hemagglutinins / Q000494:pharmacology; D007109:Immunity / Q000187:drug effects; D008297:Male; D051379:Mice; D037342:Pertussis Toxin; D010567:Pertussis Vaccine / Q000276:immunology*; D013045:Species Specificity; D015164:Vaccines, Inactivated / Q000276:immunology; D010566:Virulence Factors, Bordetella / Q000276:immunology / Q000494:pharmacology*
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D006388:Hemagglutinins; D010567:Pertussis Vaccine; D015164:Vaccines, Inactivated; D010566:Virulence Factors, Bordetella; D037342:Pertussis Toxin