Study of proton spin-lattice relaxation variation induced by paramagnetic antibodies.
A murine anti-human melanoma monoclonal antibody fragment was labeled with gadolinium and its proton relaxation efficiency compared to controls at frequencies ranging from 2 to 300 MHz. Relaxation time variations were about 30-40% in 10-15 microM solutions. The labeled fragment showed proton relaxation enhancement relative to free gadolinium, while preserving its immunoreactivity. A tentative labeling of a melanoma pellet by means of the fragment, just at the borderline of a minimum expected T1 variation, gave no detectable difference.
Macrì
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MA
Dipt. di Fisica, Università di Roma La Sapienza, Italy.
De Luca
F
F
Maraviglia
B
B
Polizio
F
F
Garreffa
G
G
Cavallo
S
S
Natali
P G
PG
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000912:Antibodies, Neoplasm / Q000032:analysis*; D001666:Binding Sites, Antibody; D005682:Gadolinium; D006801:Humans; D007140:Immunoglobulin Fab Fragments; D009682:Magnetic Resonance Spectroscopy; D008545:Melanoma / Q000276:immunology*; D051379:Mice; D011522:Protons; D013997:Time Factors
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D007140:Immunoglobulin Fab Fragments; D011522:Protons; D005682:Gadolinium