A study on spin-labelled oligonucleotide synthesis and its electron spin resonance behavior in solution.
An oligonucleotide spin-labelled with 4-amino-2,2,6,6-tetramethylpiperidine-N-oxyl (4-amino-TEMPO) at the internucleotide bond (d-Tp(L)TpTpTpT) prepared by oxidation of the pentanucleotide containing the H-phosphonate diester (d-Tp(H)TpTpTpT) in the presence of 4-amino-TEMPO, was separated and identified by high-performance, reverse-phase liquid chromatography combined with detection by electron spin resonance spectroscopy. This spin-labelled oligonucleotide produced a triplet with the slightly broadened M1 = -1 ESR component, while a triplet with almost equal intensities was obtained from the spin-label. The M1 = -1 component from the labelled oligonucleotide was further broadened in the presence of poly(A) which forms a complementary double strand with this molecule.
Makino
K
K
Department of Polymer Science and Engineering, Faculty of Textile Science, Kyoto Institute of Technology, Japan.
Murakami
A
A
Nagahara
S
S
Nakatsuji
Y
Y
Takeuchi
T
T
D002853:Chromatography, Liquid; D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy; D015345:Oligonucleotide Probes / Q000138:chemical synthesis* / Q000302:isolation & purification; D012996:Solutions; D013113:Spin Labels
D003497:Cyclic N-Oxides; D015345:Oligonucleotide Probes; D012996:Solutions; D013113:Spin Labels; C015527:4-amino-TEMPO