A kinetic fluorometric assay of dipeptidyl peptidase IV in viable human blood mononuclear cells.
A continuous-rate fluorometric assay of dipeptidyl peptidase IV (DP-IV) in viable human blood mononuclear cells using 7-(L-glycyl-L-prolylamido)-4-methylcoumarin as the substrate is described. The assay method is accurate, rapid, and highly sensitive for measuring the level of cell-surface bound DP-IV activity in suspension of blood mononuclear cells, as well as of other viable cells bearing this enzyme. We believe that the kinetic assay is suitable for studying the regulation of expression and the role of plasma membrane-bound DP-IV on the cellular level.
Sedo
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Department of Biochemistry, Research Institute of Tuberculosis and Respiratory Diseases, Prague, Czechoslovakia.
Krepela
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Kasafírek
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D002469:Cell Separation; D004247:DNA / Q000097:blood; D018819:Dipeptidyl Peptidase 4; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000097:blood*; D010450:Endopeptidases / Q000097:blood; D006801:Humans; D007700:Kinetics; D007963:Leukocytes, Mononuclear / Q000201:enzymology*; D000085822:Prolyl Oligopeptidases; D012697:Serine Endopeptidases; D013050:Spectrometry, Fluorescence
D004247:DNA; D010450:Endopeptidases; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D018819:Dipeptidyl Peptidase 4; D012697:Serine Endopeptidases; C000705287:PREPL protein, human; D000085822:Prolyl Oligopeptidases