Cytochemical demonstration of glucose-6-phosphatase in human liver.
To determine the cytochemical localization of glucose-6-phosphatase in the human hepatocyte, lead - based and cerium - based media were used. By studying the effects of systematic variation of the incubation medium components, the optimal experimental conditions were determined. The exclusive localization of the cytochemical reaction in the endoplasmic reticulum and nuclear envelope, together with the results of control experiments ensured that these findings could be correlated with the phosphohydrolase activity of the multicomponent glucose-6-phosphatase system.
Benkoel
L
L
Gulian
J M
JM
Payan
M J
MJ
Choux
R
R
Brisse
J
J
Chamlian
A
A
D002563:Cerium / Q000378:metabolism; D005952:Glucose-6-Phosphatase / Q000032:analysis*; D006651:Histocytochemistry; D006801:Humans; D007854:Lead / Q000378:metabolism; D008099:Liver / Q000201:enzymology* / Q000648:ultrastructure
D007854:Lead; D002563:Cerium; D005952:Glucose-6-Phosphatase