Nuclear retinoic-acid-binding proteins and receptors in retinoic-acid-responsive cell lines.
Nuclear retinoic-acid-binding activity and the expression of retinoic acid receptor mRNA (RAR-alpha and RAR-beta) were assayed in the F9 embryonal carcinoma, HeLa, HL-60 promyelocytic leukaemia and S91 melanoma cell lines. A 4-svedberg nuclear retinoic-acid-binding activity was detected in all 4 cell lines, but the levels in the HeLa and HL-60 cells were lower than in the F9 and S91 lines. RAR-alpha mRNA was expressed in all 4 cell lines, although at a very low level in S91 cells. Conversely, RAR-beta mRNA was expressed in S91 cells and, at a lower level, in F9 cells but was undetectable in HeLa and HL-60 cells. RAR-beta, transcribed and translated in vitro from the cloned cDNA coding region, sedimented at 4 S and this suggests that the 4-svedberg nuclear retinoic-acid-binding activity may represent the retinoic acid receptors.
Daly
A K
AK
Department of Dermatology, University of Newcastle upon Tyne, Medical School, UK.
Rees
J L
JL
Redfern
C P
CP
D015152:Blotting, Northern; D002352:Carrier Proteins / Q000032:analysis*; D002460:Cell Line / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism*; D015342:DNA Probes; D015870:Gene Expression; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis; D018168:Receptors, Retinoic Acid; D014461:Ultracentrifugation
D002352:Carrier Proteins; D015342:DNA Probes; D012333:RNA, Messenger; D018168:Receptors, Retinoic Acid