title: [Optimization of the determination in urine of alanine aminopeptidase, gamma-glutamyltransferase and N acetyl-beta-D-glucosaminidase].
abstract: The authors describe the optimization of determination of alanine aminopeptidase (AAP), gamma-glutamyltransferase (GGT) and N-acetyl-beta-D-glucosaminidase (NAG) in urine by multivariate analysis. The optimal conditions found are: for AAP at 30 degrees C TRIS HCl buffer 300 mmol/l pH 7.9, L-alanine-4-nitroanilide 5.8 mmol/l, for GGT at 30 degrees C buffer glycylglycine 150 mmol/l pH 8.0, gamma-L-glutamyl-3-carboxy-4-nitroanilide 9.0 mmol/l, for NAG at 37 degrees C citrate buffer 50 mmol/l pH 5.8, m cresolsulfonphtaleinyl-N-acetyl-beta-D-glucosaminide 5.5 mmol/l. These methods are easy to perform, apply to urine without pretreatment through Sephadex: therefore complete automatization is possible. The stability of enzymatic activities in urine is of ten days at +4 degrees C in the presence of sodium azide at neutral pH. Freezing resulted in a considerable loss of activity for AAP and GGT.
authors.affiliation: Laboratoire de Biochimie, Hôpital Debrousse, Lyon.
authors.forename: C
authors.initials: C
authors.lastname: Flandrois
authors.forename: C
authors.initials: C
authors.lastname: Lahet
authors.forename: D
authors.initials: D
authors.lastname: Feldmann
authors.forename: I
authors.initials: I
authors.lastname: Maire
authors.forename: P
authors.initials: P
authors.lastname: Aymard
authors.forename: M
authors.initials: M
authors.lastname: Mathieu
mesh_terms: D000118:Acetylglucosaminidase / Q000378:metabolism / Q000652:urine*; D000626:Aminopeptidases / Q000378:metabolism / Q000652:urine*; D000704:Analysis of Variance; D018826:CD13 Antigens; D006596:Hexosaminidases / Q000652:urine*; D006801:Humans; D005723:gamma-Glutamyltransferase / Q000378:metabolism / Q000652:urine*
chemical_list: D005723:gamma-Glutamyltransferase; D006596:Hexosaminidases; D000118:Acetylglucosaminidase; D000626:Aminopeptidases; D018826:CD13 Antigens
vernacular_title: Optimisation de la mesure dans l'urine de l'alanine aminopeptidase, de la gamma-glutamyltransférase et de la N-acétyl-bêta-D-glucosaminidase.