Carbohydrate-specific monoclonal antibodies bind to human granulocytes and stimulate antibody-dependent cellular cytotoxicity.
Mouse monoclonal antibodies which specifically recognize human granulocytes are used to study the classification, differentiation, and function of these cells. Mouse monoclonal antibody WEM-G1 specifically binds to human neutrophils and eosinophils. It also affects granulocyte function by stimulating granulocyte-mediated antibody-dependent cellular cytotoxicity. Biochemical studies presented here show that WEM-G1 recognizes the sugar sequence 3-fucosyllactosamine, Gal beta 1-4[Fuc alpha 1-3]GlcNAc. This sequence is present in granulocyte glycolipids and in glycoproteins of average approximate Mr 165,000 and 105,000. WEM-G1 is thus similar to other monoclonal antibodies that recognize this sequence on granulocytes and various other cells. Some of these 3-fucosyllactosamine-specific antibodies affect several other granulocyte functions. Knowledge of the biochemical structure of the WEM-G1 antigen suggested testing granulocyte function with other monoclonal antibodies of similar specificity. Antibodies recognizing both the identical oligosaccharide structure and a related sequence, Gal beta 1-4GlcNAc-R, were also found to stimulate granulocyte-mediated antibody-dependent cellular cytotoxicity.
Spitalnik
P F
PF
Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia 19104.
Spitalnik
S L
SL
Danley
J M
JM
Lopez
A F
AF
Vadas
M A
MA
Civin
C I
CI
Ginsburg
V
V
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000920:Antibody-Dependent Cell Cytotoxicity; D001667:Binding, Competitive; D015153:Blotting, Western; D002236:Carbohydrate Conformation; D002241:Carbohydrates / Q000276:immunology*; D004804:Eosinophils / Q000276:immunology; D006017:Glycolipids / Q000276:immunology*; D006023:Glycoproteins / Q000276:immunology; D006098:Granulocytes / Q000276:immunology*; D006801:Humans; D016256:Lewis X Antigen / Q000276:immunology; D051379:Mice; D009504:Neutrophils / Q000276:immunology; D009844:Oligosaccharides / Q000276:immunology; D011233:Precipitin Tests
D000911:Antibodies, Monoclonal; D002241:Carbohydrates; D006017:Glycolipids; D006023:Glycoproteins; D016256:Lewis X Antigen; D009844:Oligosaccharides; C045442:lacto-N-fucopentaose I