Effects of calcitonin gene-related peptide on airway epithelial functions in dogs.
We studied the effects of calcitonin gene-related peptide (CGRP) on ciliary beat frequency (CBF) and electrical properties of canine tracheal epithelium by a photoelectric method and Ussing's short-circuit technique, respectively. CGRP dose dependently increased CBF, an effect that was accompanied by elevation of intracellular cyclic AMP but not affected by blockade of either Ca2+-influx or arachidonic acid metabolism. In contrast, CGRP elicited only a small and transient increase in short-circuit current without significant alterations in transepithelial potential difference or tissue conductance. These results suggest that CGRP may play a role in regulating airway mucociliary transport function.
Tamaoki
J
J
First Department of Medicine, Tokyo Women's Medical College, Japan.
Kanemura
T
T
Kobayashi
K
K
Sakai
N
N
Takizawa
T
T
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000502:physiology*; D002923:Cilia / Q000502:physiology; D046508:Culture Techniques; D000242:Cyclic AMP / Q000378:metabolism; D004285:Dogs; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism / Q000502:physiology; D005260:Female; D008297:Male; D008564:Membrane Potentials / Q000502:physiology; D009068:Movement / Q000502:physiology; D014132:Trachea / Q000166:cytology / Q000378:metabolism / Q000502:physiology*
D000242:Cyclic AMP; D015740:Calcitonin Gene-Related Peptide