title: The pharmacology of arachidonic acid-induced rat PMN leukocyte infiltration.
abstract: Arachidonic Acid (AA) injected into a hindpaw of Lewis rats produces high levels of tissue myeloperoxidase (MPO), a biochemical marker for PMN leukocytes. Treatment with a corticosteroid (prednisolone) or dual 5-LO/CO inhibitors of AA metabolism (phenidone, SKF 86002) produced dose-related inhibition of AA-induced elevations in paw tissue MPO levels. In contrast, administration of high pharmacologic doses of selective cyclooxygenase inhibitors (indomethacin, ibuprofen, naproxen), anti-histamine/serotonin agents (cyproheptadine, chlorpheniramine) or an anti-arthritic gold compound (auranofin) produced only slight or moderate effects. Thus, AA-induced hindpaw inflammation is a useful method for determining pharmacologic effects of 5-LO/CO inhibitors on PMN leukocyte infiltration in vivo.
authors.affiliation: Smith Kline & French Laboratories, King of Prussia, PA 19406-0939.
authors.forename: M J
authors.initials: MJ
authors.lastname: DiMartino
authors.forename: C E
authors.initials: CE
authors.lastname: Wolff
authors.forename: G K
authors.initials: GK
authors.lastname: Campbell
authors.forename: N
authors.initials: N
authors.lastname: Hanna
mesh_terms: D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects; D004487:Edema / Q000201:enzymology / Q000473:pathology; D008297:Male; D009504:Neutrophils / Q000187:drug effects*; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D013997:Time Factors
chemical_list: D001095:Arachidonic Acids; D016718:Arachidonic Acid; D009195:Peroxidase