title: Cytocidal effect caused by the envelope glycoprotein of a newly isolated avian hemangioma-inducing retrovirus.
abstract: We isolated a field strain of avian hemangioma retrovirus (AHV) which induces a cytopathic effect (CPE) on cultured avian and mammalian cells shortly after infection. The kinetics of cell killing were dependent on the multiplicity of infection. The CPE on avian and mammalian cells was independent of virus replication, because UV-irradiated virus led to cell death as well. Biochemical and genetic experiments indicated that AHV env gene products were responsible for the CPE. Partially purified AHV envelope glycoproteins (gp85), but not those of the Rous sarcoma virus Prague C strain, induced a CPE. Rous-associated virus type 1, in which the env region was replaced by the AHV gp85 region, induced a CPE on avian and mammalian cultured cells. Therefore, we suggest that CPE is induced by AHV via interaction between viral gp85 and the cell membrane. This mode of CPE is unique among avian sarcoma-leukemia viruses.
authors.affiliation: Department of Molecular Genetics, Hadassah Medical School, Hebrew University, Jerusalem, Israel.
authors.forename: N
authors.initials: N
authors.lastname: Resnick-Roguel
authors.forename: H
authors.initials: H
authors.lastname: Burstein
authors.forename: J
authors.initials: J
authors.lastname: Hamburger
authors.forename: A
authors.initials: A
authors.lastname: Panet
authors.forename: A
authors.initials: A
authors.lastname: Eldor
authors.forename: I
authors.initials: I
authors.lastname: Vlodavsky
authors.forename: M
authors.initials: M
authors.lastname: Kotler
mesh_terms: D001355:Alpharetrovirus / Q000472:pathogenicity*; D000818:Animals; D001354:Avian Leukosis Virus / Q000472:pathogenicity; D002478:Cells, Cultured; D002642:Chick Embryo; D003588:Cytopathogenic Effect, Viral; D006023:Glycoproteins / Q000633:toxicity*; D006391:Hemangioma / Q000662:veterinary*; D011201:Poultry Diseases / Q000209:etiology*; D014759:Viral Envelope Proteins / Q000633:toxicity*
chemical_list: D006023:Glycoproteins; D014759:Viral Envelope Proteins