High plasma pancreastatinlike immunoreactivity in a patient with malignant insulinoma.
High levels of pancreastatinlike immunoreactivity were detected in the plasma (2.9 pmol/ml, greater than 200-fold the normal level), pancreas (2.9 nmol/g wet wt, greater than 450-fold the normal level), and liver (1.6 nmol/g wet wt) of a patient with pancreatic insulinoma with metastasis to the liver by a sensitive and specific radioimmunoassay for human pancreastatin. Antiserum was produced against the C-terminal fragment of human pancreastatin-(24-52), which was synthesized according to the sequence of human chromogranin A corresponding to that of pancreastatin. With the antiserum, intense immunocytochemical staining was detected in the tumors. Sephadex G-50 gel filtration showed that the tumors and plasma contained two molecular forms of pancreastatinlike immunoreactivity--a molecular form coeluted with synthetic human pancreastatin-52 and a larger molecular form (Mr approximately 12,000-15,000). The smaller form eluted in the same position as synthetic human pancreastatin-52 on reverse-phase high-performance liquid chromatography.
Tateishi
K
K
First Department of Biochemistry, School of Medicine, Fukuoka University, Japan.
Funakoshi
A
A
Jimi
A
A
Funakoshi
S
S
Tamamura
H
H
Yajima
H
H
Matsuoka
Y
Y
D007516:Adenoma, Islet Cell / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D053379:Chromogranin A; D005260:Female; D006801:Humans; D007340:Insulinoma / Q000032:analysis* / Q000556:secondary; D008099:Liver / Q000032:analysis; D008113:Liver Neoplasms / Q000032:analysis / Q000556:secondary*; D008875:Middle Aged; D010179:Pancreas / Q000032:analysis; D010187:Pancreatic Hormones; D010190:Pancreatic Neoplasms / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods
D053379:Chromogranin A; D010187:Pancreatic Hormones; C050836:pancreastatin