title: Colon ornithine decarboxylase activity following standard endoscopy preparation regimens.
abstract: Ornithine decarboxylase (ODC) catalyzes the formation of putrescine from ornithine, which is the first step in the pathway of mammalian polyamine biosynthesis. Tissue activity levels of ODC have been suggested to be a marker of risk for colorectal cancer in hereditary polyposis and in adenoma formers. We analyzed ODC activity in rectal and sigmoid colon mucosal biopsies obtained at 10 cm and at 30 cm in 40 healthy, colon cancer risk factor-free adults following three endoscopic preparation regimens: 1) no special preparation; 2) two phosphate enemas; and 3) "Colyte" lavage preparation 12 hr previously. Levels of ODC, measured in fresh tissue, were approximately twofold higher for enema preparation vs. no preparation (for log-transformed data: sigmoid, P less than 0.0001; rectum, P = 0.0001) and for enema preparation vs. lavage (sigmoid, P = 0.0002; rectum, P = 0.008). Lavage and no preparation ODC levels were not significantly different. ODC activity levels ranged from 0.00 to 352.96 pmol/mg/hr.
authors.affiliation: Cancer Prevention Program, University of Wisconsin Clinical Cancer Center, Madison.
authors.forename: R R
authors.initials: RR
authors.lastname: Love
authors.forename: A K
authors.initials: AK
authors.lastname: Verma
authors.forename: T S
authors.initials: TS
authors.lastname: Surawicz
authors.forename: J F
authors.initials: JF
authors.lastname: Morrissey
mesh_terms: D011125:Adenomatous Polyposis Coli / Q000175:diagnosis; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor; D001706:Biopsy; D012809:Colon, Sigmoid / Q000201:enzymology*; D003113:Colonoscopy; D015179:Colorectal Neoplasms / Q000175:diagnosis; D004573:Electrolytes; D004733:Enema; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D010710:Phosphates; D011092:Polyethylene Glycols; D012007:Rectum / Q000201:enzymology*; D012307:Risk Factors
chemical_list: D014408:Biomarkers, Tumor; D004573:Electrolytes; C033608:Golytely; D010710:Phosphates; D011092:Polyethylene Glycols; D009955:Ornithine Decarboxylase