Restriction fragment length polymorphisms for the renin gene in Dahl rats.
Genomic DNAs from inbred Dahl salt-hypertension sensitive (S) and inbred Dahl salt-hypertension resistant (R) rats were examined for restriction fragment length polymorphisms (RFLPs) using a rat renin cDNA probe. Eight of the 28 restriction enzymes used yielded polymorphic fragments between S and R strains. This suggests a major structural difference in or near the renin gene of Dahl rats. Some, but not all, of the polymorphisms are compatible with an insertion/deletion mutation of about 1.1 kb in size. The ratio of renin gene copy numbers for S to R was found to be 1, showing that the RFLPs are not likely to be due to gene duplication.
Dene
H
H
Department of Medicine, Medical College of Ohio, Toledo 43699.
Wang
S M
SM
Rapp
J P
JP
D000818:Animals; D015139:Blotting, Southern; D002872:Chromosome Deletion; D015342:DNA Probes; D006973:Hypertension / Q000235:genetics*; D009154:Mutation; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D051381:Rats; D011918:Rats, Inbred SHR / Q000235:genetics*; D011919:Rats, Inbred Strains / Q000235:genetics*; D012083:Renin / Q000235:genetics*; D012982:Sodium, Dietary / Q000009:adverse effects
D015342:DNA Probes; D012982:Sodium, Dietary; D012083:Renin