In vitro transcription of the mouse whey acidic protein promoter is affected by upstream sequences.
The promoter sequences from -175 to +10 of the mouse whey acidic protein (WAP) gene are a target for sequence-specific binding of nuclear proteins [(1987) Nucleic Acids Res. 15, 2103-2121]. Using in vitro transcription assays based on nuclear extracts, transcription factors were shown to bind to these sequences and promoter upstream sequences were found to stimulate transcription.
Lubon
H
H
Laboratory of Biochemistry and Metabolism, National Institutes of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892.
Pittius
C W
CW
Hennighausen
L
L
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D001667:Binding, Competitive; D002460:Cell Line; D004262:DNA Restriction Enzymes; D004268:DNA-Binding Proteins / Q000378:metabolism; D006801:Humans; D051379:Mice; D008894:Milk Proteins / Q000235:genetics*; D009687:Nuclear Proteins / Q000378:metabolism; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D008894:Milk Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors; C024471:whey acidic proteins; D004262:DNA Restriction Enzymes