[Isolation and fractionation in percoll gradient of guinea pig adrenal cortex cells and their functional characteristics].
Suspension of isolated adrenal cells from the guinea pig adrenals was prepared using two modifications of collagenase dispersion. The cells separated by percoll density gradient gave 4 bands. The fraction of adrenocorticocytes has buoyant density of 1.03-1.05 g/ml. The lower bands comprise blood cells with density of 1.07-1.10 g/ml. The aldosterone content in the adrenocorticocyte fraction is 1200-1300 pg/10(5) cells. Intensity of labelling of cellular proteins increases only in the adrenocorticocyte fraction in response to a rise in K+ concentration in incubation medium. The Scatchard plot method was used to characterize binding of 125I-ACTH to dispersed adrenocortical cells. Binding association constant (Ka) and binding capacity (Bmax) are 1.3 X 10(8) M-1 and 2.0 X 10(-9) mol/l, respectively. A relation between functional activity of adrenocortical cells, their ultrastructural features and density is discussed.
Tron'ko
N D
ND
Pushkarev
V M
VM
Bogdanov
T I
TI
Sautin
Iu Iu
IuIu
Mikosha
A S
AS
D000302:Adrenal Cortex / Q000166:cytology* / Q000502:physiology; D000324:Adrenocorticotropic Hormone / Q000378:metabolism; D000450:Aldosterone / Q000096:biosynthesis; D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002469:Cell Separation / Q000379:methods; D003102:Colloids; D006168:Guinea Pigs; D007202:Indicators and Reagents; D008297:Male; D011205:Povidone / Q000494:pharmacology; D014176:Protein Biosynthesis; D012822:Silicon Dioxide / Q000494:pharmacology
D003102:Colloids; D007202:Indicators and Reagents; D000450:Aldosterone; C016039:Percoll; D012822:Silicon Dioxide; D000324:Adrenocorticotropic Hormone; D011205:Povidone
Poluchenie i fraktsionirovanie v gradiente perkolla kletok kory nadpochechnikov morskikh svinok i kharakteristika ikh funktsional'nogo sostoianiia.