Mobilization of intracellular calcium by endothelin in Swiss 3T3 cells.
When intracellular free Ca2+ concentration [( Ca2+]i) was monitored in fura2-loaded Swiss 3T3 cells, endothelin increased [Ca2+]i in a dose-dependent manner; after the addition of endothelin, an initial transient peak was observed immediately and was followed by a sustained increase in [Ca2+]i lasting at least 5 min. 45Ca2+ efflux and influx experiments in endothelin-stimulated Swiss 3T3 cells revealed that the change in [Ca2+]i could be explained by a dual mechanism; an initial transient peak induced mainly by the release of Ca2+ from intracellular stores and the sustained increase by an influx of extracellular Ca2+. Cellular generation of inositol 1,4,5-trisphosphate and cyclic AMP were not induced by endothelin, suggesting that other cellular mediators with the capacity to release Ca2+ from intracellular stores play a significant role in the signal transduction pathway of endothelin in Swiss 3T3 cells.
Ohnishi
A
A
Growth Factor Division, National Cancer Center Research Institute, Tokyo, Japan.
Yamaguchi
K
K
Kusuhara
M
M
Abe
K
K
Kimura
S
S
D000818:Animals; D002118:Calcium / Q000502:physiology*; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism; D016232:Endothelins; D019886:Gastrin-Releasing Peptide; D007295:Inositol Phosphates / Q000378:metabolism; D051379:Mice; D010455:Peptides / Q000378:metabolism / Q000502:physiology*
D016232:Endothelins; D007295:Inositol Phosphates; D010455:Peptides; D019886:Gastrin-Releasing Peptide; D000242:Cyclic AMP; D002118:Calcium