Analysis of separate isolates of Bordetella pertussis repeated DNA sequences.
Two independent isolates of a Bordetella pertussis repeated DNA unit were sequenced and shown to be an insertion sequence element with five nucleotide differences between the two copies. The sequences were 1053 bp in length with near-perfect terminal inverted repeats of 28 bp, had three open reading frames, and were each flanked by short direct repeats. The two insertion sequences showed considerable homology to two other B. pertussis repeated DNA sequences reported recently: IS481 and a 530 bp repeated DNA unit. The B. pertussis insertion sequence would appear to comprise a group of closely related sequences differing mainly in flanking direct repeats and the terminal inverted repeats. The two isolates reported here, which were from the adenylate cyclase and agglutinogen 2 regions of the genome, were numbered IS48lvl and IS48lv2 respectively.
McPheat
W L
WL
Division of Bacteriology, National Institute for Biological Standards and Control, South Mimms, Hertfordshire, UK.
Hanson
J H
JH
Livey
I
I
Robertson
J S
JS
D001483:Base Sequence; D001886:Bordetella pertussis / Q000235:genetics*; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid
D004251:DNA Transposable Elements; D004269:DNA, Bacterial