Spatial and temporal expression pattern of retinoic acid receptor genes during mouse bone development.
Spatial and temporal expression pattern of retinoic acid receptor (RAR) genes was investigated in mouse finger bones during development by an in situ hybridization method with riboprobes synthesized from a human cDNA of the RAR-alpha. We found that the RAR genes are expressed intensively and specifically in calcifying fronts of the mouse finger bones, whereas the expression pattern is rather uniform in the limb buds and cartilage matrices of the embryonic fingers. Our findings are consistent with the fact that vitamin A is essential for normal mammalian bone development.
Noji
S
S
Okayama University Dental School, Okayama City, Japan.
Yamaai
T
T
Koyama
E
E
Nohno
T
T
Taniguchi
S
S
D000818:Animals; D001846:Bone Development; D001842:Bone and Bones / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics*; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D005796:Genes; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009693:Nucleic Acid Hybridization; D010012:Osteogenesis; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D018168:Receptors, Retinoic Acid; D013698:Templates, Genetic; D014158:Transcription, Genetic; D014212:Tretinoin / Q000378:metabolism
D002352:Carrier Proteins; D012333:RNA, Messenger; D018168:Receptors, Retinoic Acid; D014212:Tretinoin; D004247:DNA