Characterization of neurons containing nerve growth factor receptors in the rat neostriatum.
Using a sensitive immunohistochemical procedure, the normal morphology and distribution of neurons containing the nerve growth factor receptors (NGF-R) have been studied in the caudate-putamen of the adult rat brain, and the findings compared with observations on the choline acetyltransferase (ChAT)-positive neurons present in the immediately adjacent sections. The ChAT immunoreaction product was uniformly distributed on the cell bodies, while the NGF-R immunoreaction end product was present also as intensely stained granules (0.06-0.12 micron diameter) on the cell somata and the dendrites. In the NGF-R-positive neurons the intensity of the reaction product was much weaker than the ChAT immunoreactivity. Moreover, in comparison with ChAT-immunopositive cells, the proportion of the NGF-R-immunoreactive neurons was on average about 15% in the neostriatum.
Kiss
J
J
MRC Collaborative Centre, London, U.K.
Patel
A J
AJ
D000818:Animals; D000911:Antibodies, Monoclonal; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D003342:Corpus Striatum / Q000032:analysis* / Q000648:ultrastructure; D007124:Immunoenzyme Techniques; D008722:Methods; D009474:Neurons / Q000032:analysis* / Q000648:ultrastructure; D051381:Rats; D011956:Receptors, Cell Surface / Q000032:analysis*; D017475:Receptors, Nerve Growth Factor
D000911:Antibodies, Monoclonal; D011956:Receptors, Cell Surface; D017475:Receptors, Nerve Growth Factor; D002795:Choline O-Acetyltransferase