[Establishment and characterization of a tumor marker producing cell line (JR-1) derived from a gastric scirrhous cancer].
Despite the importance of in vitro study of gastric cancer, the established cell lines derived from human gastric carcinoma are very few. We have recently established a new cell line derived from human gastric cancer which has the ability to produce several tumor markers. This cell line has been designated JR-1. The cancer cells were obtained from the cerebrospinal fluid of a 37 year-old female patient who had metastatic brain tumor of the poorly differentiated gastric adenocarcinoma. The cells were inoculated into the tissue culture flask containing Ham's F-12 medium supplemented with 10% fetal bovine serum, antibiotics. Within 24 hours, the cells attached to the surface of the flask and started to grow. The first subcultures were performed at 1 week, and subsequent subcultures have been done once a week. This cell line has been maintained for more than 15 months through 60 passages with a stable growth. Chromosome analysis of the cells was performed. The doubling time of the 20th passage was 72 hours. Under phase contrast microscopy, monolayered pavement-like cell arrangement was observed. PAS staining showed intracellular mucin granules. Transmission and scanning electron microscopy revealed spindle-shaped cells with numerous microvilli and fine projections as well as intracellular granules, indicating mucin. Tumor markers produced by this cell line were CEA, CA19-9, TPA and Procollagen III.
Terano
A
A
Second Department of Medicine, School of Medicine, University of Tokyo, Japan.
Nakada
R
R
Mutoh
H
H
Hiraishi
H
H
Ota
S
S
Kimura
K
K
Aoyama
H
H
Sugimoto
T
T
Shiga
J
J
Itoh
Y
Y
D002293:Adenocarcinoma, Scirrhous / Q000378:metabolism / Q000473:pathology*; D000328:Adult; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000096:biosynthesis; D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D002272:Carcinoembryonic Antigen / Q000096:biosynthesis; D002455:Cell Division; D005260:Female; D006801:Humans; D008854:Microscopy, Electron; D011347:Procollagen / Q000096:biosynthesis; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology*; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000473:pathology
D015295:Antigens, Tumor-Associated, Carbohydrate; D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D011347:Procollagen; D010959:Tissue Plasminogen Activator