title: Nucleotide sequence of a neurovirulent variant of the type 2 oral poliovirus vaccine.
abstract: Infectious cDNAs of the Sabin type 2 poliovirus vaccine virus and a vaccine-derived neurovirulent type 2 strain (P2/117) have been cloned in Escherichia coli. Nucleotide sequence analysis revealed that P2/117 differs from the vaccine strain by just 23 point mutations. Three occur in the 5' noncoding region. The remainder result in a total of 5 coding changes located in VP1, VP4, 2B, and 3D. The likely role of these mutations in the evolution to neurovirulence is discussed.
authors.affiliation: Department of Microbiology, University of Reading, England.
authors.forename: S R
authors.initials: SR
authors.lastname: Pollard
authors.forename: G
authors.initials: G
authors.lastname: Dunn
authors.forename: N
authors.initials: N
authors.lastname: Cammack
authors.forename: P D
authors.initials: PD
authors.lastname: Minor
authors.forename: J W
authors.initials: JW
authors.lastname: Almond
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics; D005814:Genes, Viral; D014644:Genetic Variation; D009154:Mutation; D009420:Nervous System / Q000382:microbiology; D017955:Poliovirus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D011055:Poliovirus Vaccine, Oral / Q000032:analysis*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D014774:Virulence
chemical_list: D004279:DNA, Viral; D011055:Poliovirus Vaccine, Oral; D012367:RNA, Viral