Stimulation of the expression of osteogenic and chondrogenic phenotypes in vitro by osteogenin.
Osteogenin was recently purified and the amino acid sequences of tryptic peptides were determined. Osteogenin in conjunction with insoluble collagenous bone matrix induces cartilage and bone formation in vivo. To understand the mechanism of action of osteogenin, we examined its influence on periosteal cells, osteoblasts, fibroblasts, chondrocytes, and bone marrow stromal cells in vitro. Osteogenin stimulated alkaline phosphatase activity and collagen synthesis in periosteal cells. The cAMP response to parathyroid hormone in periosteal cells was increased by osteogenin. In primary cultures of calvarial osteoblasts, osteogenin stimulated alkaline phosphatase activity, the cAMP response to parathyroid hormone, and the synthesis of collagenous and noncollagenous proteins; however, cell proliferation was not affected. Osteogenin increased the production of sulfated proteoglycans in fetal rat chondroblasts and in rabbit articular chondrocytes. The present experiments demonstrate the significant influence of osteogenin in the stimulation of osteogenic and chondrogenic phenotypes in vitro.
Vukicevic
S
S
Bone Cell Biology Section, National Institute of Dental Research, Bethesda, MD 20892.
Luyten
F P
FP
Reddi
A H
AH
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D001857:Bone Matrix / Q000502:physiology; D055398:Bone Morphogenetic Protein 3; D019485:Bone Morphogenetic Proteins; D002356:Cartilage / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D004261:DNA Replication / Q000187:drug effects; D005333:Fetus; D007700:Kinetics; D008297:Male; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010281:Parathyroid Hormone / Q000494:pharmacology; D010521:Periosteum / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010641:Phenotype; D014176:Protein Biosynthesis; D011506:Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D011509:Proteoglycans / Q000096:biosynthesis; D051381:Rats; D011916:Rats, Inbred F344; D013431:Sulfates / Q000378:metabolism; D015291:Transforming Growth Factors / Q000494:pharmacology
C525759:Bmp3 protein, rat; D055398:Bone Morphogenetic Protein 3; D019485:Bone Morphogenetic Proteins; D010281:Parathyroid Hormone; D011506:Proteins; D011509:Proteoglycans; D013431:Sulfates; D015291:Transforming Growth Factors; D000242:Cyclic AMP; D000469:Alkaline Phosphatase