Thyroid hormone stimulates expression of 6-phosphofructo-2-kinase in rat liver.
The activity of liver 6-phosphofructo-2-kinase (PFK-2), the enzyme that catalyses the synthesis of fructose 2,6-bisphosphate, was markedly decreased in hypothyroid rats and partially restored after 3 days of treatment with triiodothyronine. The changes in PFK-2 activity were accompanied by parallel changes in enzyme content measured by immunotitration and in PFK-2 mRNA determined by dot blot and Northern blot hybridization with cDNA probes. It is concluded that thyroid hormone stimulates liver PFK-2 gene expression by a pre-translational mechanism.
Wall
S R
SR
Hormone and Metabolic Research Unit, International Institute of Cellular and Molecular Pathology, Brussels, Belgium.
van den Hove
M F
MF
Crepin
K M
KM
Hue
L
L
Rousseau
G G
GG
D000818:Animals; D015152:Blotting, Northern; D015342:DNA Probes; D005635:Fructosediphosphates / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D007037:Hypothyroidism / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008297:Male; D009206:Myocardium / Q000201:enzymology; D025481:Phosphofructokinase-2; D010770:Phosphotransferases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D013963:Thyroid Hormones / Q000494:pharmacology*; D014284:Triiodothyronine / Q000494:pharmacology
D015342:DNA Probes; D005635:Fructosediphosphates; D012333:RNA, Messenger; D013963:Thyroid Hormones; D014284:Triiodothyronine; D010770:Phosphotransferases; D025481:Phosphofructokinase-2