title: Some physico-chemical properties of the rigid form of the Sendai virus nucleocapsid.
abstract: The effect of some dissociation agents (SDS, beta-mercaptoethanol, urea, EDTA) on the rigid form of the Sendai virus nucleocapsid was studied. Polyacrylamide gel electrophoresis in the presence of lytic mixture (1% SDS, 2% beta-mercaptoethanol, 5 M urea, for 2 min at 100 degrees C) revealed two types of polypeptide subunits (mol. wts. 46,000 and 14,000), as well as the dissociation in the presence of 0.1% SDS only. The EDTA treatment leads to a disorganization of the protein part (10(-2) M) or of the nucleocapsid structure (5 x 10(-2) M).
authors.affiliation: Stefan S. Nicolau Institute of Virology, Bucharest, Romania.
authors.forename: R
authors.initials: R
authors.lastname: Repanovici
authors.forename: M
authors.initials: M
authors.lastname: Hristova
authors.forename: L M
authors.initials: LM
authors.lastname: Popa
mesh_terms: D002213:Capsid / Q000032:analysis / Q000187:drug effects / Q000302:isolation & purification*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D008854:Microscopy, Electron; D008970:Molecular Weight; D010222:Parainfluenza Virus 1, Human / Q000032:analysis / Q000187:drug effects / Q000302:isolation & purification*; D010455:Peptides / Q000032:analysis; D014758:Viral Core Proteins / Q000032:analysis / Q000187:drug effects / Q000302:isolation & purification*
chemical_list: D010455:Peptides; D014758:Viral Core Proteins