Glycine potentiates the stimulation of inositol phospholipid hydrolysis by excitatory amino acids in primary cultures of cerebellar neurons.
Glycine potentiates stimulation of inositol phospholipid hydrolysis by glutamate and N-methyl-D-aspartate, but not by quisqualate or carbamylcholine, in primary cultures of cerebellar granule cells. This potentiation occurs in the absence of extracellular Mg2+, but is more evident when stimulation of inositol phospholipid hydrolysis by N-methyl-D-aspartate is measured in the presence of 1 mM Mg2+. The action of glycine is not antagonized by strychnine. These results suggest that glycine acts as a positive modulator of signal transduction at a specific class of N-methyl-D-aspartate-sensitive glutamate receptors coupled to inositol phospholipid hydrolysis in cerebellar granule cells.
Nicoletti
F
F
Institute of Pharmacology, University of Catania School of Medicine, Italy.
Canonico
P L
PL
D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D002478:Cells, Cultured; D002531:Cerebellum / Q000187:drug effects / Q000378:metabolism*; D004357:Drug Synergism; D005971:Glutamates / Q000494:pharmacology; D018698:Glutamic Acid; D005998:Glycine / Q000494:pharmacology*; D006868:Hydrolysis; D007295:Inositol Phosphates / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology; D016202:N-Methylaspartate; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter / Q000187:drug effects / Q000502:physiology; D015398:Signal Transduction / Q000187:drug effects; D013403:Sugar Phosphates / Q000378:metabolism*
D000596:Amino Acids; D005971:Glutamates; D007295:Inositol Phosphates; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter; D013403:Sugar Phosphates; D001224:Aspartic Acid; D018698:Glutamic Acid; D016202:N-Methylaspartate; D008274:Magnesium; D005998:Glycine