Expression of three stage-specific transcripts of AMP deaminase during myogenesis.
AMP deaminase, a ubiquitous enzyme in eucaryotes, plays a central role in energy metabolism. In the present study, RNase protection analyses and immunoprecipitation with tissue-specific antisera were used to examine the transcripts and peptides of AMP deaminase produced during myogenesis in vivo and during myocyte differentiation in vitro. In embryonic muscle and undifferentiated myoblasts, a 3.4-kilobase (kb) transcript encoded a 78-kilodalton (kDa) AMP deaminase peptide that cross-reacted with antisera raised to the AMP deaminase isoform purified from kidney of the adult animal. In perinatal muscle and myocytes at an intermediate stage of differentiation in vitro, a 2.5-kb transcript was produced, and it encoded a 77.5-kDa AMP deaminase peptide that cross-reacted with antisera to the isoform purified from adult heart muscle. At about the time of birth, another 2.5-kb AMP deaminase transcript that encoded an 80-kDa peptide became detectable. This peptide cross-reacted with antisera to the predominant isoform purified from adult skeletal muscle.
Sabina
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Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710.
Ogasawara
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Holmes
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D000659:AMP Deaminase / Q000096:biosynthesis / Q000235:genetics*; D000818:Animals; D005786:Gene Expression Regulation; D007120:Immunochemistry; D024510:Muscle Development; D009132:Muscles / Q000201:enzymology*; D009709:Nucleotide Deaminases / Q000235:genetics*; D011061:Poly A / Q000235:genetics; D015347:RNA Probes; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D014158:Transcription, Genetic
D015347:RNA Probes; D012333:RNA, Messenger; D011061:Poly A; D009709:Nucleotide Deaminases; D000659:AMP Deaminase