Direct in vitro effect of 1,25-dihydroxyvitamin D3 on islets insulin secretion in vitamin deficient rats: influence of vitamin D3 pretreatment.
Vitamin D3 deficiency decreased glucose-induced insulin release from isolated rat islets. In vivo, vitamin D3 treatment restored the B-cell response within 3 days; this delay suggests an effect of vitamin D3 metabolites. The effect of 1,25-dihydroxyvitamin D3 was studied in vitro on isolated islets from vitamin D3 deficient rats. When it was added to the incubation medium, it increased in a dose-dependent manner islet insulin secretion. However this effect only occurred when the vitamin D3-deficient rats received at least a single injection of vitamin D3 24 hours earlier; these results sustain the hypothesis of a direct but delayed in vitro stimulation of B cell function by 1,25-dihydroxyvitamin D3.
Billaudel
B
B
Laboratoire d'Endocrinologie, Université de Bordeaux I, Talence, France.
Faure
A
A
Labriji-Mestaghanmi
H
H
Sutter
B C
BC
D000818:Animals; D000831:Animals, Newborn; D002117:Calcitriol / Q000494:pharmacology*; D002762:Cholecalciferol / Q000494:pharmacology; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051381:Rats; D012016:Reference Values; D014808:Vitamin D Deficiency / Q000503:physiopathology*
D007328:Insulin; D002762:Cholecalciferol; D002117:Calcitriol