Expression of major histocompatibility complex class I antigens in rat muscle cultures: the possible developmental role in myogenesis.
The expression of major histocompatibility complex class I antigens was demonstrated on aneurally cultured rat muscle cells. Myoblasts showed constitutive expression of class I antigens on their cell surfaces. The presence of the antigens was transitory, disappearing as myoblasts fused and differentiated into multinucleate myotubes. Furthermore, antibody against rat class I antigens showed an inhibitory effect on the generation of myotubes during muscle development. Although mature myotubes did not show any detectable levels of class I antigens on their cell surface, soluble factors from concanavalin A-activated spleen cells or interferon gamma could induce the expression of class I antigens on muscle fibers. These results suggest that the expression of class I antigens on muscle cells is not only immunologically modulated but also developmentally regulated and that the antigens may play a role in cell recognition and interactions during the fusion process of myogenesis.
Honda
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Department of Neurology, Hospital of the University of Pennsylvania, Philadelphia 19104.
Rostami
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D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005455:Fluorescent Antibody Technique; D005805:Genes, MHC Class I; D015395:Histocompatibility Antigens Class I / Q000032:analysis*; D007371:Interferon-gamma / Q000494:pharmacology; D008222:Lymphokines / Q000502:physiology; D009132:Muscles / Q000276:immunology*; D051381:Rats; D011917:Rats, Inbred Lew; D011994:Recombinant Proteins; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D018800:Thy-1 Antigens
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D015395:Histocompatibility Antigens Class I; D008222:Lymphokines; D011994:Recombinant Proteins; D018800:Thy-1 Antigens; D007371:Interferon-gamma