The adrenergic system in lymphocytes from children with cystic fibrosis.
Several in vivo and in vitro studies have suggested that children suffering from cystic fibrosis (CF) might have a general defect of beta-adrenoceptors on the cell surface which might account for an unbalanced secretory process. In order to investigate if this view holds true, we determined the beta-adrenoceptor density and affinity on lymphocytes by means of radioligand studies using 125-iodo-cyano-pindolol (125-ICYP) in 20 children with CF. Cyclic AMP (cAMP) response was also investigated after specific beta-adrenoceptor stimulation with isoprenaline (IPN) and after direct stimulation of the adenylate cyclase with forskolin in lymphocytes. Children with CF and controls have identical numbers and affinities of beta-adrenoceptors on lymphocytes. The cyclic AMP response was identical in CF- and in age-matched control children regardless whether adenylate cyclase was stimulated directly or via beta-adrenoceptors. In conclusion, the data support the view that no general adrenoceptor or adenylate cyclase defect exists in CF. As several studies have found abnormal reactions to adrenergic stimuli in CF patients, we presume that there is a defect beyond the level of adrenergic receptors and cAMP which remains to be identified.
Schuster
A
A
Zentrum für Kinderheilkunde der Universität Düsseldorf.
Elsen
A
A
Griese
M
M
Kusenbach
G
G
Reinhardt
D
D
D000262:Adenylyl Cyclases / Q000097:blood; D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D000242:Cyclic AMP / Q000097:blood; D003550:Cystic Fibrosis / Q000503:physiopathology*; D006801:Humans; D007223:Infant; D020120:Iodocyanopindolol; D008214:Lymphocytes / Q000502:physiology*; D010869:Pindolol / Q000031:analogs & derivatives / Q000493:pharmacokinetics; D011869:Radioligand Assay; D011943:Receptors, Adrenergic, beta / Q000502:physiology*
D011943:Receptors, Adrenergic, beta; D020120:Iodocyanopindolol; D010869:Pindolol; D000242:Cyclic AMP; D000262:Adenylyl Cyclases