Structure of the gene of tum- transplantation antigen P91A: the mutated exon encodes a peptide recognized with Ld by cytolytic T cells.
Mutagen treatment of mouse P815 tumor cells produces immunogenic mutants that express new transplantation antigens (tum- antigens) recognized by cytolytic T cells. We found that the gene conferring expression of tum- antigen P91A contains 12 exons, encoding a 60 kd protein lacking a typical N-terminal signal sequence. The sequence shows no significant similarity with sequences in current data bases. A mutation that causes expression of the antigen is located in exon 4; it is the only apparent difference between the normal and the antigenic alleles. A short synthetic peptide corresponding to a region of exon 4 located around this mutation makes P815 cells sensitive to lysis by anti-P91A cytolytic T cells. The mutation creates a strong aggretope enabling the peptide to bind the H-2 Ld molecule. Several secondary tumor cell variants that no longer express tum- antigen P91A were found to carry deletions in the gene.
Lurquin
C
C
Ludwig Institute for Cancer Research, Brussels, Belgium.
Van Pel
A
A
Mariamé
B
B
De Plaen
E
E
Szikora
J P
JP
Janssens
C
C
Reddehase
M J
MJ
Lejeune
J
J
Boon
T
T
D000595:Amino Acid Sequence; D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D001483:Base Sequence; D002460:Cell Line; D005091:Exons; D014644:Genetic Variation; D006183:H-2 Antigens / Q000276:immunology*; D006649:Histocompatibility Antigens / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D013489:Suppression, Genetic; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000276:immunology / Q000378:metabolism
D000951:Antigens, Neoplasm; D006183:H-2 Antigens; D006649:Histocompatibility Antigens; C070850:transplantation antigen P91A, mouse