The migration anomaly of DNA fragments in polyacrylamide gels allows the detection of small sequence-specific DNA structure variations.
Curved DNA fragments have a reduced electrophoretic mobility in polyacrylamide gels. The retardation in gels is extremely sensitive to small structural variations which influence the DNA helix axis. This gel assay can also be used to detect very small structural variations in DNA sequences which are not curved: The noncurved sequences of interest can be combined with curved stretches in phase with the helix turn. Using such sequence constructions, even subtle influences on the DNA helix axis can be detected. Experiments of this kind allow the determination of a relative order of sequence-specific DNA twist and wedge angles.
Diekmann
S
S
Max-Planck-Institut für Biophysikalische Chemie, Göttingen, Federal Republic of Germany.
D001482:Base Composition; D004247:DNA / Q000032:analysis* / Q000138:chemical synthesis; D004262:DNA Restriction Enzymes; D004278:DNA, Superhelical / Q000032:analysis / Q000138:chemical synthesis; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D008745:Methylation; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000032:analysis / Q000138:chemical synthesis; D012965:Sodium Chloride; D013696:Temperature
D004278:DNA, Superhelical; D009841:Oligonucleotides; D012965:Sodium Chloride; D004247:DNA; D004262:DNA Restriction Enzymes