Transcription initiation and a RNA polymerase binding site upstream of the purE gene of the archaebacterium Methanobacterium thermoautotrophicum strain delta H.
DNA-dependent RNA-polymerase (RNAP) purified from the thermophilic archaebacterium Methanobacterium thermoautotrophicum strain delta H has been shown to bind specifically to DNA in the intergenic region upstream of the purE gene cloned from this species. The RNAP binding site has been limited to a 41 bp region of DNA which contains the Box A archaebacterial promoter sequence, 5'ATTAAATA. Transcription of the purE gene appears to initiate in vivo at three locations 20-22 bp downstream of the Box A sequence and 27-29 bp upstream of the ATG translation initiation codon of the purE gene.
Brown
J W
JW
Molecular, Cellular and Developmental Biology Program, Ohio State University, Columbus 43210.
Reeve
J N
JN
D001483:Base Sequence; D001665:Binding Sites; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics* / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004720:Endonucleases; D019605:Euryarchaeota / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010957:Plasmids; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014158:Transcription, Genetic
D015342:DNA Probes; D004269:DNA, Bacterial; D012321:DNA-Directed RNA Polymerases; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases