Denaturation of poliovirus procapsids.
The denaturation of poliovirus procapsids at pH 6.5 was studied, both in the frozen and liquid condition. Denaturation involved alteration of antigenic and physical features (isoelectric pH, alkali dissociability, sedimentation coefficient). Magnesium was a stabilizing factor, and the sedimentation coefficient was the most denaturation-sensitive feature.
Rombaut
B
B
Department of Microbiology and Hygiene, Vrije Universiteit Brussel, Belgium.
Vrijsen
R
R
Boeyé
A
A
D002213:Capsid / Q000276:immunology; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D008274:Magnesium / Q000494:pharmacology; D017955:Poliovirus; D011489:Protein Denaturation; D011498:Protein Precursors / Q000276:immunology; D013696:Temperature; D014461:Ultracentrifugation
D011498:Protein Precursors; D008274:Magnesium