Rat mesangial cells actively produce phosphatidylinositol-anchored Thy-1.
We provide evidence that the mesangial cells of rat kidney glomeruli express Thy-1 as a phosphatidylinositol-anchored protein. Both the mesangial area of kidney, examined in tissue sections, and mesangial cells maintained in culture for more than 3 months, showed prominent immunofluorescence staining with an anti-Thy-1 monoclonal antibody (OX7); this staining was almost completely abolished by pretreating kidney sections or mesangial cells with the phosphatidylinositol-specific enzyme, phospholipase C. By Northern blotting, mesangial cells were shown to express mRNA of an appropriate size, hybridizing to a mouse Thy-1.1-specific probe.
Miyata
T
T
Department of Immunology, Nagoya University School of Medicine, Japan.
Isobe
K
K
Inagi
R
R
Taguchi
R
R
Ikezawa
H
H
Takai
I
I
Fujita
Y
Y
Iwamoto
T
T
Hasegawa
T
T
Oda
O
O
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D002478:Cells, Cultured; D005920:Glomerular Mesangium / Q000276:immunology*; D010716:Phosphatidylinositols / Q000276:immunology*; D051381:Rats; D018800:Thy-1 Antigens
D000954:Antigens, Surface; D010716:Phosphatidylinositols; D018800:Thy-1 Antigens