title: High pressure liquid chromatography of cyclic nucleotide phosphodiesterase from purified human T-lymphocytes.
abstract: The cyclic nucleotide phosphodiesterase (EC 3.1.4.17) in extracts of purified human peripheral blood T-lymphocytes was examined by ion exchange high pressure liquid chromatography. Four peaks of activity were isolated. The first peak of activity selectively hydrolyzed cyclic GMP. The following 3 peaks of activity (Ia, IIa and IIIa) were selective for cyclic AMP. The selective low Km cyclic AMP-phosphodiesterase inhibitor, Ro 20-1724 (d,1-1,4-[3-butoxy-4-methoxybenzyl]-2-imidazolidinone), did not inhibit the activity in Ia whereas it did inhibit the activity in IIa and IIIa (IC50 = 17 microM). The authors conclude that ion exchange high pressure liquid chromatography described in this communication is a useful method for the isolation of different forms of cyclic nucleotide phosphodiesterase activity from human T-lymphocytes.
authors.affiliation: Department of Pharmacology and Therapeutics, University of South Florida, College of Medicine, Tampa 33612-4799.
authors.forename: S A
authors.initials: SA
authors.lastname: Robicsek
authors.forename: J J
authors.initials: JJ
authors.lastname: Krzanowski
authors.forename: A
authors.initials: A
authors.lastname: Szentivanyi
authors.forename: J B
authors.initials: JB
authors.lastname: Polson
mesh_terms: D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000302:isolation & purification / Q000378:metabolism; D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000302:isolation & purification; D012368:4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone / Q000494:pharmacology; D000818:Animals; D002147:Calmodulin / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D051381:Rats; D013601:T-Lymphocytes / Q000201:enzymology*
chemical_list: D002147:Calmodulin; D012368:4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D015106:3',5'-Cyclic-GMP Phosphodiesterases