Radiation leukemia virus and its effect on H-2 gene expression.
In this report we demonstrate that lowered expression of the H-2 antigens on RadLV-induced tumour cells is a result of depressed levels of stable mRNA in these cells. Whether this observation is a result of lowered transcription or of mRNA instability is under investigation. In an effort to determine which viral sequences are essential for mediating both the H-2 regulatory function and the transforming function of RadLV, we have begun to assemble newly integrated proviral genomes from tumours. The restriction enzyme cleavage sites of four isolates are presented; these isolates differ substantially from RadLV genomes previously presented. One of these molecular clones is shown to encode a non-defective B-tropic, ecotropic virus which when reinjected into resistant mouse strains can mediate the up-regulation of H-2Dd antigen expression. Finally, possible mechanisms of H-2 regulation are discussed.
Brown
G D
GD
Department of Pathology, New York University Medical Center, NY 10016.
Meruelo
D
D
D000818:Animals; D002472:Cell Transformation, Viral / Q000235:genetics / Q000276:immunology*; D004279:DNA, Viral / Q000235:genetics; D015967:Gene Expression Regulation, Viral / Q000235:genetics / Q000276:immunology; D006183:H-2 Antigens / Q000096:biosynthesis / Q000235:genetics*; D009052:Leukemia Virus, Murine / Q000235:genetics*; D051379:Mice; D012333:RNA, Messenger / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D015533:Transcriptional Activation
D004279:DNA, Viral; D006183:H-2 Antigens; D012333:RNA, Messenger