Modulation of IL-1-induced collagenase production in articular chondrocytes by pertussis toxin.
The effects of pertussis and cholera toxins on interleukin-1 (IL-1) stimulated collagenase production from rabbit articular chondrocytes were investigated. Cholera toxin (50-1000 ng/ml) had no significant effect on IL-1-stimulated collagenase production. Pertussis toxin gave a 50-100% inhibition of collagenase activity induced by submaximal IL-1 concentrations. However, pertussis toxin had little effect on collagenase activity induced by maximal concentrations of IL-1. Optimal inhibitory effects were observed with 5-10 ng/ml of toxin.
Crawford
A
A
Research Department, Boots Company PLC, Nottingham, UK.
Crossley
M J
MJ
D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D002478:Cells, Cultured; D002772:Cholera Toxin / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology*; D003012:Microbial Collagenase / Q000096:biosynthesis*; D037342:Pertussis Toxin; D011817:Rabbits; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
D007375:Interleukin-1; D010566:Virulence Factors, Bordetella; D002772:Cholera Toxin; D037342:Pertussis Toxin; D003012:Microbial Collagenase