title: Interactions of thyroid hormones with L-(3H) glutamate binding sites, with special reference to N-methyl-D-aspartate receptors.
abstract: An L-(3H)glutamate binding assay was developed in which 82% of the specific binding is to a site that corresponds to the N-methyl-D-aspartate receptor. The effects of various anticonvulsants and thyroid hormones were investigated with this assay. Anticonvulsants did not affect L-(3H)glutamate binding, while L- and D-isomers of triiodothyronine and thyroxine inhibited L-(3H)glutamate binding significantly. Scatchard analysis showed that thyroid hormones interacted with L-(3H)glutamate binding competitively. The interactions of thyroid hormones with NMDA-sensitive L-(3H)glutamate binding may be one of the mechanisms by which these hormones affect the central nervous system.
authors.affiliation: Department of Pediatrics, Faculty of Medicine, Kyoto University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Oguro
authors.forename: M
authors.initials: M
authors.lastname: Ito
authors.forename: H
authors.initials: H
authors.lastname: Tsuda
authors.forename: K
authors.initials: K
authors.lastname: Mutoh
authors.forename: H
authors.initials: H
authors.lastname: Shiraishi
authors.forename: Y
authors.initials: Y
authors.lastname: Shirasaka
authors.forename: H
authors.initials: H
authors.lastname: Mikawa
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology; D001667:Binding, Competitive / Q000187:drug effects; D004594:Electrophysiology; D005971:Glutamates / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D017470:Receptors, Glutamate; D016194:Receptors, N-Methyl-D-Aspartate; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D013570:Synaptic Membranes / Q000187:drug effects / Q000378:metabolism; D013963:Thyroid Hormones / Q000494:pharmacology*
chemical_list: D000596:Amino Acids; D000927:Anticonvulsants; D005971:Glutamates; D017470:Receptors, Glutamate; D016194:Receptors, N-Methyl-D-Aspartate; D017981:Receptors, Neurotransmitter; D013963:Thyroid Hormones