title: Rat brain capillary endothelial cells express functional PDGF B-type receptors.
abstract: Immunohistochemical staining revealed the presence of platelet-derived growth factor (PDGF) B-type receptors on capillaries of normal rat brain. Furthermore, capillary endothelial cells isolated from rat brain and grown in tissue culture bound [125I]PDGF-BB but not [125I]PDGF-AA, suggesting that they expressed B-type, but not A-type, PDGF receptors. PDGF-BB and PDGF-AB, but not PDGF-AA, also stimulated incorporation of [3H]thymidine into these cells. Thus, rat brain capillary endothelial cells have functional B-type receptors, and thereby differ from endothelial cells derived from large blood vessels, that do not express PDGF receptors. Our data suggest a possible role for PDGF-BB as an angiogenic factor.
authors.affiliation: Ludwig Institute for Cancer Research, Biomedical Center, Uppsala, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Smits
authors.forename: M
authors.initials: M
authors.lastname: Hermansson
authors.forename: M
authors.initials: M
authors.lastname: Nistér
authors.forename: I
authors.initials: I
authors.lastname: Karnushina
authors.forename: C H
authors.initials: CH
authors.lastname: Heldin
authors.forename: B
authors.initials: B
authors.lastname: Westermark
authors.forename: K
authors.initials: K
authors.lastname: Funa
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000098:blood supply*; D002196:Capillaries / Q000187:drug effects / Q000378:metabolism; D046508:Culture Techniques; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D007150:Immunohistochemistry; D010982:Platelet-Derived Growth Factor / Q000145:classification / Q000378:metabolism* / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000145:classification / Q000187:drug effects / Q000378:metabolism*; D017479:Receptors, Platelet-Derived Growth Factor; D013936:Thymidine / Q000378:metabolism
chemical_list: D043925:Angiogenesis Inducing Agents; D010982:Platelet-Derived Growth Factor; D011956:Receptors, Cell Surface; D017479:Receptors, Platelet-Derived Growth Factor; D013936:Thymidine