title: Location of sites of transposon Tn916 insertion in the Mycoplasma mycoides genome.
abstract: The genome of Mycoplasma mycoides subsp. mycoides GC1176-2 was cleaved into six large fragments by the endonuclease KpnI which also cleaved the transposon Tn916 once. This has allowed genomic mapping of insertion sites for 50 transformants of GC1176-2 containing Tn916. Almost all of the mapped sites were clearly separate. The transformants provide a bank of genomes each with a KpnI site at a different position to facilitate mapping of gene loci.
authors.affiliation: Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Australia.
authors.forename: J C
authors.initials: JC
authors.lastname: Whitley
authors.forename: L R
authors.initials: LR
authors.lastname: Finch
mesh_terms: D002874:Chromosome Mapping; D004251:DNA Transposable Elements; D005798:Genes, Bacterial; D015698:Genomic Library; D009176:Mycoplasma mycoides / Q000235:genetics*; D015183:Restriction Mapping
chemical_list: D004251:DNA Transposable Elements