Light-induced DNA cleavage by esperamicin and neocarzinostatin.
Ultraviolet radiation of the enediyne drugs is effective in causing nicks in supercoiled DNA. Of special interest is the fact that the observed nucleotide cleaving specificity for the UV light- and thiol-activated antibiotics was the same with esperamicin A1, but was different with neocarzinostatin. In addition to the preferred cutting of T and A bases, the light-activated neocarzinostatin attacked certain G bases which were rarely cleaved by the thiol-activated neocarzinostatin. It should be noted that these enediyne antibiotics lose the DNA breakage activity after light-exposure for 30 min.
Uesawa
Y
Y
Institute for Chemical Research, Kyoto University, Japan.
Kuwahara
J
J
Sugiura
Y
Y
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D001483:Base Sequence; D004247:DNA / Q000187:drug effects* / Q000528:radiation effects; D004249:DNA Damage; D004578:Electron Spin Resonance Spectroscopy; D053281:Enediynes; D004926:Escherichia coli; D008969:Molecular Sequence Data; D010957:Plasmids / Q000187:drug effects* / Q000528:radiation effects; D014466:Ultraviolet Rays; D009353:Zinostatin / Q000494:pharmacology*
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; D053281:Enediynes; D004247:DNA; D009353:Zinostatin; C047543:esperamicin A1