Effect of gonadotrophin-releasing hormone and related analogue on human luteal cell function in vitro.
The possible direct effect of gonadotrophin-releasing hormone (GnRH) and GnRH agonist (GnRH-A; buserelin) on basal and human chorionic gonadotrophin (HCG)-stimulated progesterone (P) and cyclic AMP (cAMP) production by cultured human luteal cells was examined. Luteal cells from the early or mid-luteal phase were incubated in long-term cultures. They responded to HCG stimulation with a 2- to 3-fold increase in P production and a 2-fold increase in cAMP production. The addition of GnRH (10(-7) and 10(-5) M) or GnRH-A (10(-7) and 10(-5) M) to the medium had no effect on either basal or HCG-stimulated secretion. These results indicate that both GnRH and GnRH-A have no direct effect on human luteal steroidogenesis in vitro.
Lanzone
A
A
Department of Obstetrics and Gynaecology, Universita' Cattolica, S. Cuore, Rome, Italy.
Panetta
V
V
Di Simone
N
N
Arno E
Fulghesu
A M
AM
Caruso
A
A
Mancuso
S
S
D000328:Adult; D002478:Cells, Cultured; D003338:Corpus Luteum / Q000187:drug effects*; D000242:Cyclic AMP / Q000096:biosynthesis; D005260:Female; D006801:Humans; D008184:Luteal Cells / Q000187:drug effects* / Q000378:metabolism; D008875:Middle Aged; D010060:Ovulation; D010906:Pituitary Hormone-Releasing Hormones / Q000494:pharmacology*; D011374:Progesterone / Q000096:biosynthesis
D010906:Pituitary Hormone-Releasing Hormones; D011374:Progesterone; D000242:Cyclic AMP