GTP hydrolysis by guinea pig liver transglutaminase.
Homogeneous guinea pig liver transglutaminase was purified from a commercially available enzyme preparation by affinity chromatography on GTP-agarose. The purified transglutaminase exhibited a single band of apparent Mr = 80,000 on sodium dodecyl sulfate polyacrylamide gel and Western blotting and had enzyme activity of both transglutaminase and GTPase. The guinea pig liver transglutaminase has an apparent Km value of 4.4 microM for GTPase activity. GTPase activity was inhibited by guanine nucleotides in order GTP-gamma-S greater than GDP, but not by GMP. These results demonstrate that purified guinea pig liver transglutaminase catalyzes GTP hydrolysis.
Lee
K N
KN
Biomedical Division, Samuel Roberts Noble Foundation, Inc., Ardmore, Oklahoma 73402.
Birckbichler
P J
PJ
Patterson
M K
MK
D000818:Animals; D015153:Blotting, Western; D020558:GTP Phosphohydrolases / Q000378:metabolism; D006150:Guanine Nucleotides / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism*; D006168:Guinea Pigs; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D011503:Transglutaminases / Q000302:isolation & purification / Q000378:metabolism*
D006150:Guanine Nucleotides; D006160:Guanosine Triphosphate; D011503:Transglutaminases; D020558:GTP Phosphohydrolases