title: Wall effects in sucrose density gradient centrifugation of viruses.
abstract: The depletion of sedimenting particles to be expected from wall effects in the course of sucrose gradient centrifugation, was computed for various types of swing-out rotors on the assumption that particles hitting the tube wall are lost from the gradient. This assumption was roughly verified for heat-denatured, but not for native poliovirus. Tween 20 reduced the depletion, and prevented tight sticking of heat-denatured virus particles to tube walls.
authors.affiliation: Department of Microbiology and Hygiene, Vrije Universiteit Brussel, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Boeyé
authors.forename: A
authors.initials: A
authors.lastname: De Rees
mesh_terms: D002213:Capsid / Q000302:isolation & purification; D002499:Centrifugation, Density Gradient / Q000295:instrumentation; D006358:Hot Temperature; D008433:Mathematics; D008962:Models, Theoretical; D017955:Poliovirus / Q000302:isolation & purification; D014461:Ultracentrifugation; D014780:Viruses / Q000302:isolation & purification*