The gene for the human mast cell high-affinity IgE receptor alpha chain: chromosomal localization to Iq21-q23 and RFLP analysis.
We have used a cDNA probe for the human IgE receptor alpha chain to determine the chromosomal location for the human gene. A combination of Southern blot analysis of panels of somatic-cell hybrid DNAs and chromosomal in situ hybridization has localized the gene to the long arm of chromosome 1 at q21-q23. With this cDNA probe, we have also identified an RsaI RFLP which is inherited in a Mendelian fashion. This polymorphic marker on chromosome 1 should be valuable for studies directed at the identification both of diseases involving the IgE receptor alpha chain and of genetic factors affecting the allergic response.
Tepler
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Department of Genetics, Harvard Medical School, Howard Hughes Medical Institute, Boston MA 02115.
Morton
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CC
Shimizu
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Holcombe
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RF
Eddy
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Shows
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Leder
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P
D000944:Antigens, Differentiation, B-Lymphocyte / Q000235:genetics*; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D003001:Cloning, Molecular; D015342:DNA Probes; D015252:Deoxyribonucleases, Type II Site-Specific; D006801:Humans; D006822:Hybrid Cells; D007073:Immunoglobulin E; D008407:Mast Cells / Q000502:physiology*; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length; D011961:Receptors, Fc / Q000235:genetics*; D017455:Receptors, IgE
D000944:Antigens, Differentiation, B-Lymphocyte; D015342:DNA Probes; D011961:Receptors, Fc; D017455:Receptors, IgE; D007073:Immunoglobulin E; D015252:Deoxyribonucleases, Type II Site-Specific; C040158:GTAC-specific type II deoxyribonucleases