Spin trapping of the superoxide anion: complications in the use of the water-soluble nitroso-aromatic reagent DBNBS.
Sodium 3,5-dibromo-4-nitrosobenzenesulfonate (DBNBS) is reported to be a useful spin trap for the measurement of superoxide anions in aqueous solution. However, the signal observed arises from interaction of the spin trap with some species other than the superoxide radical or hydrogen peroxide, a product of its dismutation, as the addition of both superoxide dismutase and catalase to a superoxide generating system failed to attenuate the signal. Therefore caution must be employed in the interpretation of results obtained using this spin trap.
Mani
V
V
Department of Ophthalmology, Medical University of South Carolina, Charleston 29425.
Crouch
R K
RK
D001557:Benzenesulfonates; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D009603:Nitroso Compounds; D012995:Solubility; D013113:Spin Labels; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000032:analysis*; D014867:Water
D001557:Benzenesulfonates; D009603:Nitroso Compounds; D013113:Spin Labels; D014867:Water; D013481:Superoxides; C048757:3,5-dibromo-4-nitrosobenzenesulfonate; D013482:Superoxide Dismutase