Evidence for a single class of somatostatin receptors in ground squirrel cerebral cortex.
In the present study we characterized high-affinity somatostatin (SRIF) binding sites (Kd = 2.06 +/- 0.32 nM and Bmax = 295 +/- 28 fmol/mg protein) in cerebral cortex membrane preparations of European ground squirrel using 125I-[Tyr0-D-Trp8]-SRIF14 as a radioligand. The inhibition of radioligand specific binding by SRIF14, as well as by its agonists (SRIF28, Tyr0-D-Trp8-SRIF14, SMS 201 995) was complete and monophasic, thus revealing a single population of somatostatinergic binding sites. Radioautographic analysis of 125I-[Tyr0-D-Trp8]-SRIF14 labeled brain sections confirmed the results of our biochemical study. The homogeneity of SRIF binding sites in the ground squirrel neocortex was not dependent on the animal's life-cycle phase.
Krantic
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S
Institut for Biological Research Dr S. Stankovic, Department of Endocrinology and Metabolism, Beograd, Yugoslavia.
Petrovic
V M
VM
Quirion
R
R
Kordon
C
C
D000818:Animals; D001143:Arousal; D001345:Autoradiography; D001667:Binding, Competitive; D002540:Cerebral Cortex / Q000378:metabolism*; D006605:Hibernation; D007457:Iodine Radioisotopes; D007700:Kinetics; D008297:Male; D051381:Rats; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D017481:Receptors, Somatostatin; D012589:Sciuridae; D013004:Somatostatin / Q000378:metabolism*
D007457:Iodine Radioisotopes; D017981:Receptors, Neurotransmitter; D017481:Receptors, Somatostatin; D013004:Somatostatin