title: Ultrastructural localization of calcium binding sites on human muscle cell surface.
abstract: Calcium (Ca2+) is mainly bound to anionic phospholipids and to sialic acid at the cell surface. We studied the ultrastructural localization of these Ca2+ binding sites in normal human muscle fibers, using Polymyxin B as a marker for anionic phospholipids and the lectin Limulus Polyphemus as a probe for sialic acid. We found that anionic phospholipids have a patchy distribution along the muscle sarcolemma, with a preferential localization at the I band level and at the junction between the I and A band. Sialic acid has an uniform distribution along the muscle plasma membrane and basal lamina. Our observations suggest that the plasma membrane, basal lamina, and transverse tubular system play an important role in providing the negative charge of the human muscle cell surface and that these structures may be involved in the binding of calcium.
authors.affiliation: Istituto di Clinica Neurologica, Centro Dino Ferrari, Universita' degli Studi di Milano, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Moggio
authors.forename: S
authors.initials: S
authors.lastname: Jann
authors.forename: L
authors.initials: L
authors.lastname: Adobbati
authors.forename: A
authors.initials: A
authors.lastname: Prelle
authors.forename: A
authors.initials: A
authors.lastname: Gallanti
authors.forename: G
authors.initials: G
authors.lastname: Fagiolari
authors.forename: G
authors.initials: G
authors.lastname: Pellegrini
authors.forename: G
authors.initials: G
authors.lastname: Scarlato
mesh_terms: D060829:Arthropod Proteins; D001665:Binding Sites; D015220:Calcium Channels / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D006801:Humans; D037102:Lectins; D009132:Muscles / Q000166:cytology / Q000378:metabolism*; D011112:Polymyxin B
chemical_list: D060829:Arthropod Proteins; D015220:Calcium Channels; D037102:Lectins; D011112:Polymyxin B