title: NADH-sensitive propionyl-CoA hydrolase in brown-adipose-tissue mitochondria of the rat.
abstract: Acyl-CoA hydrolase activity was studied in brown adipose tissue (BAT) mitochondria of rats. The substrate specificity was investigated: total hydrolase activity showed two activity peaks, one sharp peak for propionyl-CoA and a broad peak at medium- to long-chain acyl-CoAs. The propionyl-CoA activity fully comigrated with a mitochondrial matrix marker enzyme in fractionation studies of tissue and mitochondria. The hydrolytic activity against short-chain acyl-CoAs was inhibited by NADH, and analyses of the substrate specificity of the hydrolases in the presence and absence of NADH allowed for the delineation of two distinct acyl-CoA hydrolases. These hydrolases could also be separated by gel filtration. It was concluded that rat BAT mitochondria possess at least two matrix acyl-CoA hydrolases: one broad-spectrum acyl-CoA hydrolase with an apparent native molecular weight of less than 100,000, and a specific propionyl-CoA hydrolase with an apparent native molecular weight at least 240,000; this hydrolase is regulated by NADH. It is suggested that the function of the propionyl-CoA hydrolase is to ensure that the level of propionyl-CoA in the mitochondria is not detrimentally increased.
authors.affiliation: Wenner-Gren Institute, University of Stockholm, Sweden.
authors.forename: S E
authors.initials: SE
authors.lastname: Alexson
authors.forename: L T
authors.initials: LT
authors.lastname: Svensson
authors.forename: J
authors.initials: J
authors.lastname: Nedergaard
mesh_terms: D002001:Adipose Tissue, Brown / Q000201:enzymology*; D000818:Animals; D002850:Chromatography, Gel; D005260:Female; D007700:Kinetics; D008928:Mitochondria / Q000201:enzymology*; D009243:NAD / Q000494:pharmacology*; D010084:Oxidation-Reduction; D010170:Palmitoyl-CoA Hydrolase / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity; D013869:Thiolester Hydrolases / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D009243:NAD; D013869:Thiolester Hydrolases; C061158:propionyl CoA hydrolase; D010170:Palmitoyl-CoA Hydrolase