[Resistance of heart valve bioprosthesis to the proteolytic effect of collagenase and elastase].
Proteolytic destruction of pig aortal valves was studied at various steps of their preparation to implantation (native tissue, the tissue treated with terrylytine as well as with terrylytine and glutaric aldehyde) using pig pancreatic elastase and bacterial collagenase. The rate of the tissue destruction was estimated by means of monitoring an increase in content of protein and amino nitrogen in the hydrolysates. The tissue treated with terrylytine and glutaricaldehyde was 10-40-fold more resistant to proteolysis as compared with native heart valve tissue. Inadequate stabilizing effect of glutaric aldehyde on elastin, as compared with that on collagen, was found, when proteolysis of native and modified with glutaric aldehyde elastin from bovine cervical ligament and of calf skin collagen was studied using elastase and collagenase, respectively.
Krasovskaia
S M
SM
Platonova
L V
LV
Dzemeshkevich
S L
SL
D001705:Bioprosthesis; D003094:Collagen / Q000378:metabolism; D004549:Elastin / Q000378:metabolism; D006350:Heart Valve Prosthesis; D006801:Humans; D006868:Hydrolysis; D066298:In Vitro Techniques; D003012:Microbial Collagenase / Q000494:pharmacology*; D010196:Pancreatic Elastase / Q000494:pharmacology*; D011475:Prosthesis Failure
D003094:Collagen; D004549:Elastin; D010196:Pancreatic Elastase; D003012:Microbial Collagenase
Izuchenie ustoĭchivosti bioprotezov klapanov serdtsa k proteoliticheskomu vozdeĭstviiu kollagenazy i élastazy.