Regulation of the gamma-aminobutyric acidA receptor by gamma-aminobutyric acid levels within the postsynaptic cell.
Synaptosomes and synaptoneurosomes were prepared from rat cerebral cortex. Comparison of the amino acid levels in the two types of organelles and of the effects of gabaculine thereon indicated that the neurosome portion of synaptoneurosomes constituted the major influencing component of the organelles. Administration to rats of inhibitors of gamma-aminobutyric acid (GABA) degradation, such as gabaculine and L-cycloserine, resulted in elevated GABA levels in synaptoneurosomes and a decrease in muscimol-stimulated Cl- up-take by the organelles. Addition of gabaculine directly to the incubation medium for the uptake assay had no effect on the Cl- transport. In contrast, administration to rats of isonicotinic acid hydrazide, an inhibitor of GABA synthesis, decreased the GABA level in synaptoneurosomes and increased the muscimol-stimulated Cl- uptake by the organelles. Although the evidence is not unequivocal, it does support the concept of GABA released from nerve endings being taken up by the postsynaptic cell, from where it exerts a regulatory influence on the functioning of the GABA receptor/ion channel complex.
Wood
J D
JD
Department of Biochemistry, University of Saskatchewan, Saskatoon, Canada.
Davies
M
M
D000818:Animals; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D002712:Chlorides / Q000378:metabolism; D003470:Culture Media; D003509:Cyclohexanecarboxylic Acids / Q000494:pharmacology; D008297:Male; D009474:Neurons / Q000378:metabolism; D015388:Organelles / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011963:Receptors, GABA-A / Q000378:metabolism*; D013569:Synapses / Q000378:metabolism*; D013574:Synaptosomes / Q000378:metabolism; D013654:Taurine / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D002712:Chlorides; D003470:Culture Media; D003509:Cyclohexanecarboxylic Acids; D011963:Receptors, GABA-A; D013654:Taurine; C012987:gabaculine; D005680:gamma-Aminobutyric Acid