Rapid detection of herpes simplex virus DNA by in situ hybridization with photobiotin-labelled double-stranded DNA probes.
An assay for rapid detection of herpes simplex virus in infected cells is described. The assay utilizes in situ hybridization with photobiotin-labelled double-stranded DNA probes prepared from HSV-1 DNA cloned in plasmid vectors. The assay provided an alternative method for earlier detection of virus in cell cultures with the ease of preparation of photobiotin-labelled double-stranded DNA.
Chantratita
W
W
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Henchal
E A
EA
Yoosook
C
C
D000818:Animals; D001386:Azides; D001710:Biotin / Q000031:analogs & derivatives*; D004247:DNA; D015342:DNA Probes; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000032:analysis*; D006561:Herpes Simplex / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D018139:Simplexvirus / Q000235:genetics / Q000302:isolation & purification*; D014709:Vero Cells; D014776:Virus Cultivation
D001386:Azides; D015342:DNA Probes; D004274:DNA, Recombinant; D004279:DNA, Viral; D001710:Biotin; D004247:DNA; C045549:photobiotin