title: Molecular cloning of a full-length cDNA for human alpha-N-acetylgalactosaminidase (alpha-galactosidase B).
abstract: In the process of molecular cloning of cDNA for proteins associated with a purified human placental sialidase fraction, we discovered one of the proteins with apparent molecular weight of 46 kDa is in reality alpha-N-acetylgalactosaminidase. The full length cDNA, pcD-HS1204, codes for 358 amino acids with the first 17 residues representing a putative signal peptide. The predicted amino acid sequence shows striking homology with human alpha-galactosidase A and yeast alpha-galactosidase. The substrate specificities as well as the behavior of the 46 kDa protein on hydroxylapatite chromatography confirmed that the 46 kDa protein is in reality alpha-N-acetylgalactosaminidase.
authors.affiliation: Department of Neurology, Brain Research Institute, Niigata University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Tsuji
authors.forename: T
authors.initials: T
authors.lastname: Yamauchi
authors.forename: M
authors.initials: M
authors.lastname: Hiraiwa
authors.forename: T
authors.initials: T
authors.lastname: Isobe
authors.forename: T
authors.initials: T
authors.lastname: Okuyama
authors.forename: K
authors.initials: K
authors.lastname: Sakimura
authors.forename: Y
authors.initials: Y
authors.lastname: Takahashi
authors.forename: M
authors.initials: M
authors.lastname: Nishizawa
authors.forename: Y
authors.initials: Y
authors.lastname: Uda
authors.forename: T
authors.initials: T
authors.lastname: Miyatake
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002845:Chromatography; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D017886:Durapatite; D005260:Female; D006596:Hexosaminidases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D006882:Hydroxyapatites; D007700:Kinetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy; D048809:alpha-N-Acetylgalactosaminidase
chemical_list: D006882:Hydroxyapatites; D004247:DNA; D017886:Durapatite; D006596:Hexosaminidases; C482722:NAGA protein, human; D048809:alpha-N-Acetylgalactosaminidase