Effects of interferon-beta on Daudi cells and on small cell lung carcinoma cells which over-express the c-myc oncogene.
The effects of human interferon-beta (IFN-beta) on four lung cancer cell lines, two of which over-express the c-myc oncogene, were compared to Daudi cells which also over-express c-myc. The anti-proliferative effect of IFN-beta on Daudi cells was correlated with a decrease in steady state levels of c-myc mRNA. IFN-beta treatment of a classic small cell lung carcinoma cell line and a non-small cell lung carcinoma cell line which express normal levels of c-myc did not decrease the rate of cell proliferation. IFN-beta, however, was found to have dramatic anti-proliferative effects on the two lung cancer cell lines with c-myc over-expression. The growth inhibitory effects of IFN-beta on the two lung cancer cell lines could not, however, be correlated with decreased amounts of c-myc mRNA. Furthermore, Western blot analysis showed that interferon treatment did not detectably affect the c-myc protein levels in these cells. Therefore, the mechanism by which IFN-beta slowed the growth rates of the two lung cancer cell lines did not appear to directly involve down-regulation of the c-myc oncogene, expressed in amplified amounts, in these cells.
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Department of Zoology, Arizona State University, Tempe 85287-1501.
Floyd-Smith
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D018288:Carcinoma, Small Cell; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004261:DNA Replication / Q000187:drug effects; D015870:Gene Expression; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D007700:Kinetics; D008175:Lung Neoplasms; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc; D011519:Proto-Oncogenes / Q000187:drug effects; D011994:Recombinant Proteins; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000166:cytology* / Q000187:drug effects
D007370:Interferon Type I; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D011994:Recombinant Proteins; D011505:Protein-Tyrosine Kinases; D013936:Thymidine