Adrenergic stimulation of inositol phosphate accumulation in tracheal epithelium.
To determine whether inositol phosphates are important second messengers in the regulation of Cl- secretion by airway epithelia, we examined the relationship between inositol phosphate accumulation and Cl- secretion in response to adrenergic agonists. We found that epinephrine stimulated Cl- secretion and inositol phosphate accumulation with similar concentration dependence. Although isoproterenol stimulated Cl- secretion, there was no effect of beta-adrenergic receptor activation on inositol phosphate accumulation. In contrast, alpha 1-adrenergic receptor activation stimulated inositol phosphate accumulation but failed to induce Cl- secretion. Another Cl- secretagogue, prostaglandin E1, also failed to stimulate inositol phosphate accumulation. These data suggest that inositol phosphate accumulation is neither sufficient nor required for stimulation of Cl- secretion in cultured canine tracheal epithelial cells.
Bainbridge
T
T
Department of Internal Medicine, University of Iowa College of Medicine, Iowa City 52242.
Feldman
R D
RD
Welsh
M J
MJ
D000316:Adrenergic alpha-Agonists / Q000494:pharmacology*; D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D002712:Chlorides / Q000378:metabolism / Q000494:pharmacology; D004285:Dogs; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D007295:Inositol Phosphates / Q000378:metabolism*; D008094:Lithium / Q000494:pharmacology; D018021:Lithium Chloride; D013268:Stimulation, Chemical; D013403:Sugar Phosphates / Q000378:metabolism*; D014132:Trachea / Q000166:cytology / Q000378:metabolism*
D000316:Adrenergic alpha-Agonists; D000318:Adrenergic beta-Agonists; D002712:Chlorides; D007295:Inositol Phosphates; D013403:Sugar Phosphates; D008094:Lithium; D018021:Lithium Chloride