[The isolation of a concentrated and purified rotavirus preparation by using bentonite].
The preparation obtained when cultivating virus SA-11 in the transplantable cell culture SPEV has been purified and concentrated by means of bentonite. The virus adsorption at acidic values of pH (4.0-4.5) and elution at alkaline values of pH (8.5) were the main stages of production of the concentrated and purified rotavirus preparation. The paper embraces data on the infective activity of the virus protein content, amount of the rotavirus antigen, results of the electron and immunoelectron microscopy, characterizing the method efficiency.
Girin
V N
VN
Dziublik
I V
IV
Boĭko
I I
II
Gnatovskaia
S I
SI
Plugatyr'
V M
VM
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001546:Bentonite; D003588:Cytopathogenic Effect, Viral; D008854:Microscopy, Electron; D012401:Rotavirus / Q000276:immunology / Q000302:isolation & purification* / Q000472:pathogenicity / Q000648:ultrastructure; D014773:Virology / Q000379:methods; D014776:Virus Cultivation / Q000379:methods
D000956:Antigens, Viral; D001546:Bentonite
Poluchenie kontsentrirovannogo i ochishchennogo preparata rotavirusa s pomoshch'iu bentonita.