title: Lymphocyte recognition of psoriatic endothelium: evidence for a tissue-specific receptor/ligand interaction.
abstract: Epidermotropic lymphocytes are an essential cellular component of the skin-associated lymphoid tissues (SALT). Dermal lymphocytic infiltrates are also characteristics of inflammatory dermatoses, such as psoriasis, and may be involved in the pathogenesis of the disease, although the mechanisms by which lymphocytes are recruited into these sites are not known. We have used an in vitro lymphocyte adherence assay to test the hypothesis that specialized endothelial cells are present in inflamed skin, and are capable of supporting lymphocyte adherence and promoting lymphocyte emigration. In this assay, we assessed the binding of lymphocytes overlaid onto frozen sections of normal and psoriatic skin. Peripheral blood mononuclear cells (PBMC) from patients, from healthy volunteers, and from rat thoracic duct bound specifically to dermal endothelia in psoriatic plaques, in steroid-resistant areas of plaques, but not in uninvolved skin or skin from healthy individuals. Analysis of the binding properties of lymphocyte subsets revealed preferential adherence by CD4+ T cells as compared with CD8+ T cells and to B cells. Interestingly, PBMC from patients undergoing ultraviolet light therapy failed to adhere to autologous skin or to lesion-containing skin sections from untreated patients. Additional studies indicate that the lymphocyte-endothelial interaction is an energy- and calcium-dependent process and involves surface glycoprotein and carbohydrate moieties, requirements similar to those found in specific lymphocyte interactions with high endothelial venules in lymph nodes during the homing process. Pretreatment of lymphocytes with antibodies directed against homing receptors mediating migration into lymph nodes and into gut-associated lymphoid tissues, however, did not interfere with lymphocyte adherence to psoriatic endothelium. In contrast, anti-lymphocyte function associated antigen (LFA)-1 antibody partially inhibited lymphocyte binding to chronic plaques. We conclude that a tissue-specific receptor/ligand interaction independent of LFA-1 directs lymphocyte emigration from the dermal microvasculature into the psoriatic dermis and that LFA-1 plays only an accessory role in the adhesion process.
authors.affiliation: Department of Microbiology and Immunology, University of Miami School of Medicine, Florida 33101.
authors.forename: Y H
authors.initials: YH
authors.lastname: Chin
authors.forename: V
authors.initials: V
authors.lastname: Falanga
authors.forename: J W
authors.initials: JW
authors.lastname: Streilein
authors.forename: R
authors.initials: R
authors.lastname: Sackstein
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000528:radiation effects; D002465:Cell Movement; D002908:Chronic Disease; D003872:Dermatitis / Q000473:pathology; D004727:Endothelium / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D004730:Endothelium, Vascular / Q000378:metabolism / Q000503:physiopathology; D004817:Epidermis / Q000378:metabolism / Q000503:physiopathology; D006801:Humans; D008024:Ligands / Q000378:metabolism*; D008214:Lymphocytes / Q000502:physiology*; D011565:Psoriasis / Q000378:metabolism / Q000503:physiopathology*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012016:Reference Values; D012867:Skin / Q000098:blood supply / Q000473:pathology / Q000503:physiopathology; D014466:Ultraviolet Rays
chemical_list: D008024:Ligands; D011956:Receptors, Cell Surface