Temperature and synaptic efficacy in frog skeletal muscle.
1. Intracellular recording and voltage-clamp techniques were used to measure synaptic efficacy and the safety factor for neuromuscular transmission in frog skeletal muscle. All measurements were made in normal Ringer solution, in the absence of presynaptic or postsynaptic blocking agents. 2. Over a broad temperature range (10-30 degrees C), a small percentage of sartorius fibres (about 6%) could be found which produced only subthreshold end-plate potentials and no action potential in response to single, supramaximal nerve shock. At lower temperatures the proportion of such fibres increased; 42% of the fibres had subthreshold transmission at 5 degrees C, and 59% were subthreshold at 2.5 degrees C. 3. Threshold current, measured by intracellularly injecting short pulses of depolarizing current at end-plate regions, was independent of temperature between 2.5 and 20 degrees C. Thus, the reduced synaptic efficacy observed at low temperatures was not due to decreased electrical excitability of the postsynaptic membrane. 4. The amplitude of evoked end-plate currents (EPCs) decreased with cooling. At temperatures below 10 degrees C, the evoked EPCs at many end-plates were too small to initiate action potentials. The decline in EPC amplitude was due to three factors: a decrease in the amplitude of single quantum currents (MEPCs), an increase in the temporal dispersion of transmitter release, and (below 5 degrees C) a decrease in quantal content. 5. The safety factor for neuromuscular transmission decreased dramatically as temperature was lowered. At 30 degrees C average safety factor was large and positive (540 nA), but at 2.5 degrees C it was negative (-78 nA). 6. The quantal content of evoked transmitter release was independent of temperature change between 5 and 30 degrees C, the average value over this range being 180. However, at temperatures below 5 degrees C, quantal content fell off sharply (average value = 37). 7. The thermal independence of transmitter release may be an important mechanism in allowing poikilothermic animals to maintain physiological function over a wide range of body temperatures.
Adams
B A
BA
Department of Developmental & Cell Biology, University of California, Irvine 92717.
D000200:Action Potentials; D000818:Animals; D005071:Evoked Potentials; D005260:Female; D008297:Male; D008564:Membrane Potentials; D009469:Neuromuscular Junction / Q000502:physiology*; D013569:Synapses / Q000502:physiology*; D009435:Synaptic Transmission; D013696:Temperature; D014982:Xenopus laevis