Monoclonal antibodies that specifically inhibit GM-CSF- and IL-3-dependent growth of human monocytic leukemia cells.
We describe monoclonal antibodies (mAbs: anti-MaG-1, TGI-1, TGI-5, and TGI-6) that block the proliferation of AML-193 cells in response to GM-CSF or IL-3 and do not affect the proliferation of AML-193 cells in response to G-CSF and IL-2-driven proliferation of Kit 225 cells. However, none of the mAbs tested had any stimulative effect on the proliferation of AML-193 cells. The mAbs (anti-MaG-1, TGI-1, -5, and -6) could inhibit the binding of [125I]GM-CSF to AML-193 cells. We were able to purify MaG-1 Ag by anti-MaG-1 affinity chromatography. Thus, the MaG-1 Ag and the Ags recognised by mAbs (TGI-1, -5, and -6) may be associated with the receptor for GM-CSF or IL-3 or a structure close to the receptor for GM-CSF or IL-3.
Taniyama
T
T
Department of Cellular Immunology, National Institute of Health, Tokyo, Japan.
Taki
S
S
Nagata
M
M
Yoshizawa
K
K
Hirayama
N
N
Hamuro
J
J
Uchiyama
T
T
Wong
G
G
Rovera
G
G
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D015153:Blotting, Western; D002455:Cell Division / Q000187:drug effects; D003115:Colony-Stimulating Factors / Q000276:immunology* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007377:Interleukin-3 / Q000276:immunology*; D007948:Leukemia, Monocytic, Acute / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000276:immunology; D016184:Receptors, Colony-Stimulating Factor; D011971:Receptors, Immunologic / Q000276:immunology; D016185:Receptors, Interleukin-3; D011994:Recombinant Proteins / Q000276:immunology; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D003115:Colony-Stimulating Factors; D006133:Growth Substances; D007377:Interleukin-3; D011956:Receptors, Cell Surface; D016184:Receptors, Colony-Stimulating Factor; D011971:Receptors, Immunologic; D016185:Receptors, Interleukin-3; D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor