title: Protein kinase activity associated with the large subunit of herpes simplex virus type 2 ribonucleotide reductase (ICP10).
abstract: The large subunit of the herpes simplex virus type 2 (HSV-2) ribonucleotide reductase (RR1) is demonstrated to possess serine/threonine-specific kinase activity. Computer-assisted sequence analysis identified regions within the amino terminus of ICP10 that are homologous to the catalytic domain of known protein kinases (PKs). An in vitro kinase assay confirmed the ability of ICP10, immunoprecipitated from either HSV-2-infected cells or from cells transfected with an ICP10 expression vector, to autophosphorylate and transphosphorylate exogenous substrates in the presence of ATP and Mg2+. The HSV-1 RR1 was shown to be negative for PK activity under these conditions. PK activity was localized to a 57-kDa amino-terminal region within ICP10 that lacked RR activity.
authors.affiliation: Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, Baltimore 21201.
authors.forename: T D
authors.initials: TD
authors.lastname: Chung
authors.forename: J P
authors.initials: JP
authors.lastname: Wymer
authors.forename: C C
authors.initials: CC
authors.lastname: Smith
authors.forename: M
authors.initials: M
authors.lastname: Kulka
authors.forename: L
authors.initials: L
authors.lastname: Aurelian
mesh_terms: D000465:Algorithms; D000595:Amino Acid Sequence; D000818:Animals; D002458:Cell Fractionation; D002460:Cell Line; D006801:Humans; D008838:Microcomputers; D008969:Molecular Sequence Data; D009154:Mutation; D011233:Precipitin Tests; D011494:Protein Kinases / Q000032:analysis* / Q000235:genetics; D015183:Restriction Mapping; D012264:Ribonucleotide Reductases / Q000032:analysis* / Q000235:genetics; D018139:Simplexvirus / Q000201:enzymology* / Q000235:genetics; D012984:Software; D014162:Transfection; D014709:Vero Cells
chemical_list: D012264:Ribonucleotide Reductases; D011494:Protein Kinases