Tricarboxylate carrier of bovine liver mitochondria. Purification and reconstitution.
The tricarboxylate carrier of bovine liver mitochondria has been solubilized by Triton X-114 and purified by chromatography on hydroxylapatite and Silica Gel 60. The purified carrier could be visualized as a single band in polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate with Mr 37,000-38,000. The carrier, after reconstitution in phospholipid vesicles, catalyzed the exchange of [14C]citrate against citrate, malate, and threo-D8-isocitrate and was inhibited by the specific tricarboxylate carrier inhibitor 1,2,3-benzenetricarboxylic acid.
Claeys
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Institut für Biochemie und Molekularbiologie, Universität Bern, Switzerland.
Azzi
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D000818:Animals; D002352:Carrier Proteins / Q000302:isolation & purification* / Q000378:metabolism; D002384:Catalysis; D002417:Cattle; D002850:Chromatography, Gel; D002951:Citrates; D006882:Hydroxyapatites; D008081:Liposomes; D008930:Mitochondria, Liver / Q000378:metabolism*; D017830:Octoxynol; D011092:Polyethylene Glycols; D058428:Silica Gel; D012822:Silicon Dioxide; D014233:Tricarboxylic Acids / Q000378:metabolism*
D002352:Carrier Proteins; D002951:Citrates; D006882:Hydroxyapatites; D008081:Liposomes; D014233:Tricarboxylic Acids; D011092:Polyethylene Glycols; D058428:Silica Gel; D012822:Silicon Dioxide; D017830:Octoxynol; C010615:Nonidet P-40