Bone marrow peroxidase in mice: determination of the level and the catalytic activity.
The level of myeloperoxidase and the peroxidase activity in the bone marrow were determined in the various strains of mice. The levels of myeloperoxidase, which was determined by a immunostaining coupled with sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE), were ranged from 3 to 5 micrograms per mg protein in the bone marrow of various strains of mice. No strain difference of the enzyme activity toward guaiacol was observed among mice. On the other hand, the activity toward KI of C3H/He mice was lower than C57BL/6, Balb/c and ddY mice.
Lee
E
E
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kobe-Gakuin University, Japan.
Miki
Y
Y
Yoshida
T
T
Tsuruoka
Y
Y
Kariya
K
K
D000818:Animals; D001853:Bone Marrow / Q000201:enzymology*; D002384:Catalysis; D004591:Electrophoresis, Polyacrylamide Gel; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D009195:Peroxidase / Q000276:immunology / Q000302:isolation & purification; D010544:Peroxidases / Q000032:analysis / Q000276:immunology / Q000378:metabolism*
D010544:Peroxidases; D009195:Peroxidase