Influence of diethyldithiocarbamate on nickel-induced hepatic metallothionein in rats.
The influence of sodium diethyldithiocarbamate (DDC) on 63nickel chloride-induced metallothionein (MT) and alterations in essential metal levels in the liver of rats was investigated. The induction of MT, Zn and Cu levels of the hepatic cytosolic "heat stable fraction" (HSF) by DDC increased with time up to 24 hr. Although MT, Zn and Cu were significantly greater at 17 hr than those at 6.5 hr after 63Ni administration, the Ni level decreased. The treatment with DDC at 6 hr, but not at 10 min, prior to 63Ni administration increased significantly the MT, Zn and Cu contents 17 hr after 63Ni administration over that caused by 63Ni alone at 17 hr., showing a synergistic effect. The induction of hepatic MT by 63Ni or DDC alone was prevented by cycloheximide but not by actinomycin D and seemed to be regulated at the protein synthesis level.
Khandelwal
S
S
Industrial Toxicology Research Centre, Lucknow, India.
Kachru
D N
DN
Tandon
S K
SK
D000818:Animals; D003300:Copper / Q000378:metabolism; D003513:Cycloheximide / Q000494:pharmacology; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology; D004050:Ditiocarb / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008668:Metallothionein / Q000378:metabolism*; D009532:Nickel / Q000494:pharmacology*; D051381:Rats; D013997:Time Factors; D015032:Zinc / Q000378:metabolism
D003609:Dactinomycin; D003300:Copper; D009532:Nickel; D008668:Metallothionein; D003513:Cycloheximide; D004050:Ditiocarb; D015032:Zinc