[Constitutive expression of human fibroblast interferon gene controlled by SV40 early promoter in CHO cells].
HindII fragment encoding human fibroblast interferon (IFN beta) gene coding sequence was fused at 60 bp downstream from the RNA start site of SV40 early gene to be a constitutive expression plasmid pSVE beta. This recombinant plasmid was transfected into the dihydrofolate reductase (dhfr)-deficient Chinese hamster ovary (CHO) cells together with a selectable dhfr gene. About half of transformants continuously secreted IFN beta into the supernatant without inducement. One of the subclone transformants constitutively produced up to 852U IFN beta/2 X 10(6) cells/ml. 48hr in common medium.
Wang
X M
XM
Institute of Microbiology and Epidemiology, Academy of Military Medical Science, Beijing.
Yu
M
M
Wang
J X
JX
Wu
S H
SH
Hu
Y W
YW
Hou
Y D
YD
D000818:Animals; D006224:Cricetinae; D003412:Cricetulus; D005786:Gene Expression Regulation; D006801:Humans; D007370:Interferon Type I / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D013539:Simian virus 40 / Q000235:genetics*
D007370:Interferon Type I