title: Role of hypochlorous acid in Trypanosoma musculi killing by phagocytes.
abstract: Trypanosoma musculi are readily killed when phagocytosed by mononuclear phagocytes but the nature of the mediators of this cytotoxicity is unclear. Among the most potent mediators are oxygen-derived species. The generation of chemiluminescence (CL) by peritoneal macrophages from 12 day T. musculi-infected mice, which phagocytose and kill parasites when opsonizing antibodies are present, was recorded in the presence of antibody-coated trypanosomes. Taurine, a specific quencher of hypochlorous acid (HOCl) inhibited CL production by peritoneal macrophages, showing that HOCl is produced during phagocytosis of T. musculi. In vitro, HOCl alone exerted a powerful trypanocidal activity which was inhibited in the presence of specific quenchers. The role of HOCl generated by phagocytes in trypanosome killing was studied using granulocytes which produce more oxygen-derived species than macrophages when stimulated. Phorbol myristate acetate-triggered granulocytes can destroy T. musculi and trypanosome killing is inhibited in the presence of taurine. These data demonstrate that HOCl produced by phagocytes can effectively destroy T. musculi.
authors.affiliation: Laboratoire de Parasitologie, Université de Bordeaux II, France.
authors.forename: P
authors.initials: P
authors.lastname: Vincendeau
authors.forename: S
authors.initials: S
authors.lastname: Daulouède
authors.forename: B
authors.initials: B
authors.lastname: Veyret
mesh_terms: D000818:Animals; D005260:Female; D006997:Hypochlorous Acid / Q000378:metabolism / Q000494:pharmacology*; D008163:Luminescent Measurements; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism / Q000469:parasitology; D051379:Mice; D010586:Phagocytes / Q000276:immunology* / Q000378:metabolism / Q000469:parasitology; D010587:Phagocytosis; D014345:Trypanosoma / Q000187:drug effects / Q000276:immunology*
chemical_list: D006997:Hypochlorous Acid