Organization of the yeast URA2 gene: identification of a defective dihydroorotase-like domain in the multifunctional carbamoylphosphate synthetase-aspartate transcarbamylase complex.
The 6636 bp of the yeast URA2 gene encoding the carbamoylphosphate synthetase-aspartate transcarbamylase complex have been sequenced. The protein is organized into four regions, three of which are functional domains as indicated previously by genetic analysis. The fourth domain corresponds to a defective dihydroorotase called DHOase-like. The URA2 gene complex with the same organization as the equivalent genes in higher eukaryotes suggests an evolution from a common ancestral gene.
Souciet
J L
JL
Laboratoire de Génétique Physiologique, Institut de Biologie Moléculaire et Cellulaire du C.N.R.S., Strasbourg, France.
Nagy
M
M
Le Gouar
M
M
Lacroute
F
F
Potier
S
S
D000581:Amidohydrolases / Q000235:genetics*; D000595:Amino Acid Sequence; D001221:Aspartate Carbamoyltransferase / Q000235:genetics*; D001483:Base Sequence; D002223:Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics; D004080:Dihydroorotase / Q000235:genetics*; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics*; D012331:RNA, Fungal / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic
D004271:DNA, Fungal; D009097:Multienzyme Complexes; D012331:RNA, Fungal; D012333:RNA, Messenger; C019820:carbamoyl phosphate synthase-aspartatecarbamoyl transferase complex; D001221:Aspartate Carbamoyltransferase; D000581:Amidohydrolases; D004080:Dihydroorotase; D002223:Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)