title: Induction of neutralizing antibodies to transmissible gastroenteritis virus by anti-idiotypic antibodies.
abstract: The potential of anti-idiotypic antibodies (anti-ids) as immunogens against transmissible gastroenteritis virus (TGEV) was tested in a heterologous system. A month-old pig was immunized with a neutralizing murine monoclonal antibody (MAb, 5A5) of the IgG2a isotype, specific for the E2 glycoprotein of TGEV. The anti-ids were isolated from the serum of the immunized pig by affinity chromatography, initially on a 5A5-Sepharose column, followed by repeated adsorption on a mouse IgG2a column. The swine anti-ids thus obtained bound to the MAb 5A5 (the idiotype), but not to MAbs of the same isotype IgG2a but of different idiotypes. The anti-ids also inhibited the binding of 5A5 to TGEV in a concentration-dependent manner. Mice immunized with the anti-ids produced antibodies to TGEV. These antibodies, neutralized TGEV in vitro and inhibited the binding of 5A5 to TGEV.
authors.affiliation: Department of Veterinary Science, University of Nebraska, Lincoln.
authors.forename: K
authors.initials: K
authors.lastname: Hariharan
authors.forename: S
authors.initials: S
authors.lastname: Srikumaran
authors.forename: R A
authors.initials: RA
authors.lastname: Moxley
authors.forename: F A
authors.initials: FA
authors.lastname: Osorio
authors.forename: A A
authors.initials: AA
authors.lastname: Morales
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000096:biosynthesis*; D003332:Coronaviridae / Q000276:immunology*; D005260:Female; D007114:Immunization; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D013552:Swine; D005760:Transmissible gastroenteritis virus / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D007130:Immunoglobulin Idiotypes; D014759:Viral Envelope Proteins