Computer-assisted imaging cytometry of nuclear chromatin reveals bone tumor virus infection and neoplastic transformation of adherent osteoblast-like cells.
Established osteoblast-like (OB) cells infected with the bone tumor-inducing C-type retrovirus OA MuLV remained nontumorigenic over 104 cell culture passages. DNA histograms revealed a new cell population with a stem line peak at 5c. A second OA MuLV-infected OB cell line underwent neoplastic transformation with increasing passage level. These cells showed diffuse aneuploidy. Stepwise linear discriminant analysis of the chromatin structure of control, OA MuLV-infected, and FBR osteosarcoma virus-transformed cell lines resulted in various levels of discrimination ranging between 79.6% for control cells versus nontumorigenic OA MuLV-infected cells, and 96.6% for nontumorigenic OA MuLV-infected cells versus FBR osteosarcoma virus-transformed cells. OA MuLV-infected tumorigenic cells and FBR osteosarcoma virus-transformed cells were discriminated at a 93.6% level.
Schmidt
J
J
GSF-Abteilung für Molekulare Zellpathologie, Institut für Strahlenschutz, Neuherberg, Federal Republic of Germany.
Aubele
M
M
Jütting
U
U
Rodenacker
K
K
Luz
A
A
Erfle
V
V
Burger
G
G
D000818:Animals; D000831:Animals, Newborn; D002460:Cell Line; D002467:Cell Nucleus / Q000382:microbiology* / Q000648:ultrastructure; D002471:Cell Transformation, Neoplastic; D002843:Chromatin / Q000382:microbiology* / Q000648:ultrastructure; D002999:Clone Cells; D003201:Computers; D005434:Flow Cytometry / Q000379:methods; D009052:Leukemia Virus, Murine / Q000472:pathogenicity / Q000648:ultrastructure*; D007942:Leukemia, Experimental / Q000473:pathology*; D051379:Mice; D010006:Osteoblasts / Q000382:microbiology* / Q000648:ultrastructure
D002843:Chromatin