Enhanced infectivity of bluetongue virus in cell culture by centrifugation.
The effects of centrifugation of the infection of cell culture with bluetongue virus (BTV) were investigated. Baby hamster kidney cells were infected with BTV with or without centrifugation. Viral antigen was detected by immunofluorescence at 24 h in both centrifuged and noncentrifuged cultures. However, after 24 h of infection, the production of PFU in centrifuged cell cultures was 10- to 20-fold greater than that seen in cultures not centrifuged. In addition, centrifugation enhanced the direct detection of PFU from blood samples collected from a sheep experimentally infected with BTV.
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Arthropod-Borne Animal Diseases Research Laboratory, U.S. Department of Agriculture, Laramie, Wyoming 82071-3965.
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D000818:Animals; D001820:Bluetongue virus / Q000254:growth & development / Q000502:physiology*; D002460:Cell Line; D002498:Centrifugation; D005455:Fluorescent Antibody Technique; D012087:Reoviridae / Q000502:physiology*; D014709:Vero Cells; D010948:Viral Plaque Assay; D014779:Virus Replication