title: Steady-state levels of pro-dynorphin-related end-products from the brain of the amphibian, Xenopus laevis.
abstract: Steady-state analyses of prodynorphin-derived opioid peptides were conducted on acid extracts of the brain of the frog. Xenopus laevis. Radioimmunoassays specific for dynorphin A(1-17), dynorphin A(1-8), alpha-neoendorphin and dynorphin B coupled with gel filtration chromatography and reverse phase high performance liquid chromatography were used. The major prodynorphin-related end-product detected was alpha-neoendorphin. Interestingly, Leu-enkephalin was also detected. Since the Xenopus proenkephalin precursor does not contain the Leu-enkephalin sequence, these data suggest that some of the prodynorphin-related end-products had been cleaved to yield Leu-enkephalin.
authors.affiliation: University of Denver, Department of Biological Sciences, CO 80208.
authors.forename: C A
authors.initials: CA
authors.lastname: Sei
authors.forename: R
authors.initials: R
authors.lastname: Richard
authors.forename: R M
authors.initials: RM
authors.lastname: Dores
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D004399:Dynorphins / Q000378:metabolism*; D004723:Endorphins / Q000378:metabolism*; D004745:Enkephalins / Q000378:metabolism*; D011498:Protein Precursors / Q000378:metabolism*; D014982:Xenopus laevis / Q000378:metabolism*
chemical_list: D004723:Endorphins; D004745:Enkephalins; D011498:Protein Precursors; C025681:alpha-neoendorphin; D004399:Dynorphins; C033660:preproenkephalin