Study of two different enzyme immunoassays for the detection of Mayaro virus antibodies.
This paper presents the evaluation of an enzyme immunoassay in which Mayaro virus-infected cultured cells are used as antigen (EIA-ICC) and an IgM antibody capture ELISA (MAC-ELISA) for Mayaro serologic diagnosis using 114 human sera obtained during a Mayaro outbreak occurred in Bolivia, in 1987. Results were compared with those obtained by haemagglutination-inhibition test (HAI). MAC-ELISA was the most sensitive technique for anti-Mayaro IgM detection. MAC-ELISA was twice as sensitive as IgM EIA-ICC. The data shows that MAC-ELISA is a practical and valid technique for diagnosis of recent Mayaro infection. IgG EIA-ICC showed high sensitivity and high specificity compared to HAI. The combination of anti-Mayaro IgG and IgM EIA-ICC results presented the highest sensitivity of the study. Anti-Mayaro IgG and IgM simultaneous detection by EIA-ICC can be used for recent infection diagnosis (in spite of a less sensitive IgM detection than by MAC-ELISA), for surveillance and sero-epidemiologic studies, and for studies of IgG and IgM responses to Mayaro infection.
Figueiredo
L T
LT
Departamento de Clínica Médica, Faculdade de Medicina de Ribeirão Preto, USP, Brasil.
Nogueira
R M
RM
Cavalcanti
S M
SM
Schatzmayr
H
H
da Rosa
A T
AT
D000524:Alphavirus / Q000276:immunology*; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral; D004797:Enzyme-Linked Immunosorbent Assay; D006386:Hemagglutination Tests; D006801:Humans; D014036:Togaviridae Infections / Q000175:diagnosis*
D000914:Antibodies, Viral; D000956:Antigens, Viral