Alpha-chloralose opens the chloride channel of frog isolated sensory neurons.
The effect of alpha-chloralose on the sensory neurons isolated enzymatically and mechanically from frog dorsal root ganglia was studied using a suction-pipette technique. The threshold concentration of alpha-chloralose was around 3 x 10(-5) M and the current produced by alpha-chloralose saturated at the concentration of 3 x 10(-3) M or more. The dose-response curve for alpha-chloralose provided a Ka value of 6 x 10(-4) M and a Hill coefficient of 1.8. The reversal potential of the response elicited by alpha-chloralose was close to the equilibrium potential for Cl- (ECl), indicating that the current was carried through Cl- channels. The current-voltage relationship indicated that there was little voltage dependence in the alpha-chloralose-induced response. The analysis of the variance of the alpha-chloralose-induced Cl- current fluctuations showed two types of the receptor-ionophore complexes with different channel conductances.
Ishizuka
S
S
Department of Physiology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Sikdar
S K
SK
Yasui
S
S
Oyama
Y
Y
Akaike
N
N
D000818:Animals; D002698:Chloralose / Q000494:pharmacology*; D018118:Chloride Channels; D002712:Chlorides; D005727:Ganglia, Spinal / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects; D008565:Membrane Proteins / Q000502:physiology*; D009475:Neurons, Afferent / Q000187:drug effects / Q000502:physiology*; D011892:Rana catesbeiana
D018118:Chloride Channels; D002712:Chlorides; D008565:Membrane Proteins; D002698:Chloralose