title: Isolation and characterization of the phosphoglucose isomerase gene from Escherichia coli.
abstract: The nucleotide sequence of the gene encoding the glycolytic enzyme phosphoglucose isomerase (PGI) from Escherichia coli is presented. The gene encodes a polypeptide of 549 amino acids. The transcriptional start point of the gene was determined and found to lie within a consensus promoter region. The amino acid sequence derived from the E. coli PGI gene can be aligned without insertions or deletions to the predicted amino acid sequence of a nuclear-encoded chloroplast isozyme of PGI from a higher plant, and the two sequences have a similarity of 87.6%. The amino acid sequence similarity between E. coli and that predicted from cDNA sequences for mouse and pig PGI is approximately 65%.
authors.affiliation: Department of Genetics, University of California, Davis 95616.
authors.forename: B E
authors.initials: BE
authors.lastname: Froman
authors.forename: R C
authors.initials: RC
authors.lastname: Tait
authors.forename: L D
authors.initials: LD
authors.lastname: Gottlieb
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA; D015342:DNA Probes; D004269:DNA, Bacterial; D004591:Electrophoresis, Polyacrylamide Gel; D004720:Endonucleases; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005956:Glucose-6-Phosphate Isomerase / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D010944:Plants / Q000235:genetics; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013045:Species Specificity
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial; D012333:RNA, Messenger; D004247:DNA; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases; D005956:Glucose-6-Phosphate Isomerase