Cloning and expression of the major inner capsid protein of SA-11 simian rotavirus in Escherichia coli.
The major inner capsid protein (VP6) of SA-11 simian rotavirus has been expressed in Escherichia coli using a cloned cDNA derived from SA-11 double-stranded RNA segment 6. The cloned gene was fused to the N-terminal coding sequence of lacZ resulting in the synthesis of a 44-kDa protein. Several smaller polypeptides were also observed, resulting predominantly from transcription and translation within the gene 6 coding sequence. The recombinant VP6 proved to be antigenic by immunoblot analysis using polyclonal serum against SA-11 rotavirus and by Western-blot analysis using monospecific serum derived from purified viral VP6.
Smith
R E
RE
Corporate Molecular Biology, Abbott Laboratories, Abbott Park, IL 60064.
Kister
S E
SE
Carozzi
N B
NB
D000818:Animals; D000956:Antigens, Viral; D001483:Base Sequence; D015153:Blotting, Western; D002213:Capsid / Q000096:biosynthesis* / Q000235:genetics*; D036022:Capsid Proteins; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000096:biosynthesis* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005814:Genes, Viral; D000882:Haplorhini / Q000382:microbiology; D007763:Lac Operon; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D015347:RNA Probes; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D012401:Rotavirus / Q000235:genetics*; D014162:Transfection
D000956:Antigens, Viral; D036022:Capsid Proteins; D004279:DNA, Viral; D015347:RNA Probes; D011993:Recombinant Fusion Proteins; C061332:VP6 protein, Rotavirus