Effects of hematoporphyrin derivative on the cellular energy metabolism in the absence and presence of light.
Effects of Photofrin II on energy metabolism and metabolic viability were studied in a mammalian transformed cell line (BHK-21) in dark and after photo-irradiation with visible light. Cells were allowed to accumulate Photofrin by incubating for 4 h in buffer containing Photofrin (5-60 micrograms/ml). The results show that Photofrin significantly affects the cellular energy metabolism even in the absence of light; activity of cytochrome c oxidase is decreased and glucose utilization and lactate production (glycolysis) are increased. Irradiation with light resulted in a significant decrease in the activity of cytochrome c oxidase, glycolysis, ATP content, energy charge, ratios of adenine nucleotides like ATP/ADP, ATP/AMP and cell viability (dye exclusion test). Presence of inhibitors of energy metabolism, potassium cyanide (respiration) and 2-deoxyglucose (glycolysis), further enhanced the cytotoxic effects induced by hematoporphyrin derivative and light.
Khanum
F
F
Jain
V
V
D000227:Adenine Nucleotides / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000970:Antineoplastic Agents; D002461:Cell Line, Transformed; D006224:Cricetinae; D003576:Electron Transport Complex IV / Q000378:metabolism; D004734:Energy Metabolism / Q000187:drug effects*; D005947:Glucose / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects; D017324:Hematoporphyrin Derivative; D006415:Hematoporphyrins / Q000494:pharmacology*; D007668:Kidney; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008027:Light; D011838:Radiation-Sensitizing Agents
D000227:Adenine Nucleotides; D000970:Antineoplastic Agents; D006415:Hematoporphyrins; D007773:Lactates; D011838:Radiation-Sensitizing Agents; D019344:Lactic Acid; D017324:Hematoporphyrin Derivative; D000255:Adenosine Triphosphate; D003576:Electron Transport Complex IV; D005947:Glucose