title: Large-scale mapping and chromosome jumping in the q27 region of the human X chromosome.
abstract: We have used pulsed field gel electrophoresis for further physical mapping studies in the q27 region of the human X chromosome. We show that the DXS 102 locus and the F9 gene are separated by only 300 kb despite a genetic distance of 1.4 cM; this linkage orients our large-scale map and shows that the mcf.2 transforming sequence is telomeric to F9. A BssHII complete-digest jumping library was used to jump toward the DXS 105 locus; a 130-kb jump was achieved and the corresponding "linking clone" was obtained.
authors.affiliation: Centre d'Immunologie de Marseille Luminy INSERM-CNRS, France.
authors.forename: C
authors.initials: C
authors.lastname: Nguyen
authors.forename: A M
authors.initials: AM
authors.lastname: Poustka
authors.forename: M
authors.initials: M
authors.lastname: Djabali
authors.forename: D
authors.initials: D
authors.lastname: Roux
authors.forename: J F
authors.initials: JF
authors.lastname: Mattei
authors.forename: H
authors.initials: H
authors.lastname: Lehrach
authors.forename: B R
authors.initials: BR
authors.lastname: Jordan
mesh_terms: D015139:Blotting, Southern; D002874:Chromosome Mapping; D015342:DNA Probes; D004586:Electrophoresis; D008040:Genetic Linkage; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length; D014960:X Chromosome / Q000648:ultrastructure*
chemical_list: D015342:DNA Probes