[Steady-state calcium accumulation and its reduction by caffeine in sarcoplasmic reticulum from masseter muscle].
The passive Ca2+ efflux pathways in skeletal sarcoplasmic reticulum (SR) vesicles include the efflux through a Ca2+ channel and a passive leak parallel to the channel and the pump. It is known that caffeine stimulates Ca(2+)-induced Ca2+ release. To gain further insight into the effect of caffeine on Ca2+ flux behavior of canine masseter muscle SR vesicles, the present study focuses on the interaction of steady-state Ca2+ accumulation and passive Ca2+ permeability in the presence or absence of endogenous calmodulin (CaM), which is known to regulate Ca2+ release channel. Caffeine (1) produced ruthenium red- or endogenous CaM-inhibitable reduction of oxalate-supported Ca2+ uptake velocity with no effect on Ca2+, Mg(2+)-ATPase activity; (2) reduced steady-state Ca2+ uptake; and (3) had no effect on the permeability of the SR vesicles to Ca2+, determined by measuring net efflux of Ca2+ after stopping pump mediated fluxes, suggesting that passive Ca2+ permeability is unimportant pathway for changing steady-state Ca2+ accumulation. The inhibitory effect of caffeine on steady-state Ca2+ uptake was moderately abolished by the removal of endogenous CaM from SR vesicles. Inositol 1,4,5-trisphosphate (IP3) caused the same effect as that of caffeine on oxalate-supported Ca2+ uptake velocity, steady-state Ca2+ uptake and passive Ca2+ permeability. In summary, the data reveal that caffeine (1) inhibits oxalate entry pathway via inhibition of CaM, and (2) directly modifies CaM-dependent component of Ca2+ fluxes in the SR and reduces steady-state Ca2+ accumulation due to increased Ca2+ release through a Ca2+ efflux pathway which is inhibited by CaM but not due to reduced catalytic activity of the pump; and that the masseter muscle SR vesicles include IP3-sensitive Ca2+ release channel.
Saito
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D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002110:Caffeine / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels; D002129:Calcium Oxalate / Q000378:metabolism; D002147:Calmodulin / Q000502:physiology; D004285:Dogs; D008406:Masseter Muscle / Q000378:metabolism*; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*
D015220:Calcium Channels; D002147:Calmodulin; D002129:Calcium Oxalate; D002110:Caffeine; D002118:Calcium