Participation of cyclic adenosine monophosphate and beta-adrenergic receptors in the facilitatory effect of noradrenaline on the response of cultured cerebellar neurons to glutamate.
For the purpose of examining possible involvement of the cyclic adenosine monophosphate (cAMP) system and adrenergic receptors in the modulatory effect of noradrenaline (NA) on the glutamate-induced depolarizing response, the effects of dibutyryl cAMP (DBcAMP), forskolin, theophylline, clonidine, isoproterenol and propranolol were intracellularly investigated in the cerebellar neurons cultured from chick embryos. Not only NA-induced hyperpolarization and increase in input resistance but also the facilitatory effect of NA on the glutamate response were mimicked by DBcAMP and isoproterenol. This facilitatory effect of DBcAMP was enhanced by theophylline or forskolin, while that of isoproterenol was antagonized by propranolol. Clonidine suppressed glutamate-induced depolarization. These results that the enhancing action of NA on the responsiveness of cultured cerebellar neurons to excitatory amino acids is mediated by beta-adrenergic receptors and the intracellular cAMP system.
Mori-Okamoto
J
J
Department of Physiology, National Defense Medical College, Saitama, Japan.
Tasuno
J
J
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D002642:Chick Embryo; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism / Q000502:physiology*; D005971:Glutamates / Q000494:pharmacology*; D018698:Glutamic Acid; D007545:Isoproterenol / Q000494:pharmacology; D009638:Norepinephrine / Q000494:pharmacology*; D011943:Receptors, Adrenergic, beta / Q000187:drug effects / Q000502:physiology*; D015290:Second Messenger Systems / Q000187:drug effects*; D013806:Theophylline / Q000494:pharmacology
D005971:Glutamates; D011943:Receptors, Adrenergic, beta; D005576:Colforsin; D018698:Glutamic Acid; D003994:Bucladesine; D013806:Theophylline; D000242:Cyclic AMP; D007545:Isoproterenol; D009638:Norepinephrine