title: Histamine and bradykinin stimulate the phosphoinositide turnover in human umbilical vein endothelial cells via different G-proteins.
abstract: The G-proteins which regulate hormonal turnover of phosphoinositide (PI) in human umbilical vein endothelial cells have been investigated. A 40-41 kDa doublet present in the membranes of these cells was selectively ADP ribosylated by pertussis toxin (PTx), and this doublet was Gi alpha 2 and Gi alpha 3 according to immunoblotting with specific antisera. By contrast, a doublet of 24-26 kDa proteins in the same membrane preparations was ADP ribosylated by the C3 component of botulinum toxin (BoTx). PTx-dependent ADP ribosylation blocked stimulation of PI turnover by histamine, but did not affect stimulation by bradykinin, whereas BoTx (C2 + C3 components) had the opposite effect. Thus two different groups of G-proteins may be involved in hormone-dependent stimulation of PI turnover in human umbilical vein endothelial cells.
authors.affiliation: Institute of Experimental Cardiology, Moscow, USSR.
authors.forename: T A
authors.initials: TA
authors.lastname: Voyno-Yasenetskaya
authors.forename: M P
authors.initials: MP
authors.lastname: Panchenko
authors.forename: E V
authors.initials: EV
authors.lastname: Nupenko
authors.forename: V O
authors.initials: VO
authors.lastname: Rybin
authors.forename: V A
authors.initials: VA
authors.lastname: Tkachuk
mesh_terms: D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D001905:Botulinum Toxins / Q000494:pharmacology; D001920:Bradykinin / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006150:Guanine Nucleotides / Q000494:pharmacology; D006632:Histamine / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D037342:Pertussis Toxin; D010716:Phosphatidylinositols / Q000378:metabolism*; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
chemical_list: D006150:Guanine Nucleotides; D010716:Phosphatidylinositols; D010566:Virulence Factors, Bordetella; D000246:Adenosine Diphosphate Ribose; D006632:Histamine; D037342:Pertussis Toxin; D001905:Botulinum Toxins; D019204:GTP-Binding Proteins; D001920:Bradykinin