Potentiation of the proliferative response of human B lymphocytes to low molecular weight B cell growth factor (LMW-BCGF) by fibroblast growth factors (FGFs).
Both acidic and basic fibroblast growth factor (FGF), although devoid alone of growth-promoting ability on resting or activated human lymphoid B cells, were found to markedly increase the proliferative response of anti-mu-chain or SAC preactivated B cell blasts to the low molecular weight B cell growth factor (LMW-BCGF) and to enhance the costimulatory response of resting B cells to anti-mu-chain and LMW-BCGF. This potentiating effect was also observed for a LMW-BCGF-dependent B cell tumor derived from a lymphocytic nodular lymphoma. Other growth factors acting on fibroblasts, such as epidermal growth factor, alpha-thrombin, platelet-derived growth factor, and insulin-like growth factor-I did not display such enhancing effect on LMW-BCGF-driven proliferation. Activated, but not resting B cells were found to bear receptor sites for FGFs and from kinetics experiments, it is suggested that LMW-BCGF induces competence expression for FGFs in those cells. Moreover, the LMW-BCGF-elicited generation of inositoltrisphosphate resulting from polyphosphoinositides hydrolysis was increased in the presence of FGF.
Genot
E
E
U 196 INSERM Institut Curie, Paris, France.
Petit-Koskas
E
E
Sensenbrenner
M
M
Labourdette
G
G
Kolb
J P
JP
D001402:B-Lymphocytes / Q000502:physiology*; D004357:Drug Synergism; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D015847:Interleukin-4; D007378:Interleukins / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D008970:Molecular Weight; D010716:Phosphatidylinositols / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism; D017468:Receptors, Fibroblast Growth Factor; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured
D007376:Interleukin-2; D007378:Interleukins; D010716:Phosphatidylinositols; D011956:Receptors, Cell Surface; D017468:Receptors, Fibroblast Growth Factor; D015847:Interleukin-4; D005346:Fibroblast Growth Factors