Characteristics of the cellular response of the rat conjunctiva to topically applied leukotriene B4.
Cellular events in the rat conjunctiva were studied at 1, 6 and 24 h following the topical application of leukotriene B4. A significant increase was noted in the number of eosinophils (p less than 0.01) and neutrophils (p less than 0.05) at 1 h and only in eosinophils at 6 h after leukotriene B4 application at the 2-micrograms dose. At the 200-ng dose, the eosinophil count was significantly elevated at 1 h only, and the number of neutrophils was not significantly changed at any time point. No significant increase in cell counts was observed at the 20-ng dose. In conclusion, certain doses of topically applied leukotriene B4 significantly increased the number of eosinophils and neutrophils in the rat conjunctiva.
Trocmé
S D
SD
Eye Research Institute of Retina Foundation, Harvard Medical School, Boston, Mass.
Gilbert
C M
CM
Allansmith
M R
MR
Bloch
K J
KJ
Abelson
M B
MB
D000287:Administration, Topical; D000818:Animals; D002633:Chemotaxis / Q000187:drug effects; D003228:Conjunctiva / Q000187:drug effects* / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D004804:Eosinophils / Q000187:drug effects; D007958:Leukocyte Count / Q000187:drug effects; D007975:Leukotriene B4 / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D009504:Neutrophils / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D007975:Leukotriene B4