title: Mix.1, a homeobox mRNA inducible by mesoderm inducers, is expressed mostly in the presumptive endodermal cells of Xenopus embryos.
abstract: In frogs, mesoderm presumably derives from presumptive ectoderm by induction under the control of diffusible substances produced by the endoderm. To analyze the early phase of mesoderm induction, I have isolated cDNA copies of mRNAs induced in presumptive ectoderm by mesoderm inducing factor secreted by the XTC cell line. One of the inducible mRNAs encodes a homeodomain-containing protein that is likely to play a regulatory role in development. Mix.1 behaves as an immediate early response to induction, and its kinetics of expression suggest a major role for MBT in the control of inducible gene expression. Unexpectedly, Mix.1 is expressed mostly in the future endoderm, suggesting that endoderm may be formed by induction in a similar way as mesoderm.
authors.affiliation: Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, Bethesda, Maryland 20892.
authors.forename: F M
authors.initials: FM
authors.lastname: Rosa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004627:Embryonic Induction; D004707:Endoderm / Q000502:physiology*; D005786:Gene Expression Regulation / Q000187:drug effects; D005801:Genes, Homeobox; D006133:Growth Substances / Q000494:pharmacology; D008648:Mesoderm / Q000502:physiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics; D013997:Time Factors; D014018:Tissue Distribution; D014982:Xenopus laevis / Q000196:embryology*
chemical_list: D006133:Growth Substances; D012333:RNA, Messenger; D004247:DNA