General characteristics and viral susceptibility of a newborn pig kidney (NPK) continuous culture.
We have developed a fibroblastic-like continuous culture of newborn pig kidney (NPK). The current cell line was serially passaged 160 times and appeared to be well suited for production and assay of a number of viruses affecting pigs, such as pig parvovirus, pseudorabies and transmissible gastroenteritis. The cell line appeared aneuploid, with a modal chromosome number of 36 and induced tumors, classified as fibrosarcoma, in athymic mice.
Ferrari
M
M
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia, Brescia, Italy.
Losio
M N
MN
Gualandi
G L
GL
Di Lernia
R
R
Manocchio
I
I
D000818:Animals; D000831:Animals, Newborn; D002460:Cell Line; D002470:Cell Survival; D006692:Classical Swine Fever Virus / Q000254:growth & development; D003588:Cytopathogenic Effect, Viral; D004771:Enteroviruses, Porcine / Q000254:growth & development; D005347:Fibroblasts; D011558:Herpesvirus 1, Suid / Q000254:growth & development; D007668:Kidney / Q000166:cytology*; D051379:Mice; D010321:Parvoviridae / Q000254:growth & development; D013552:Swine; D005760:Transmissible gastroenteritis virus / Q000254:growth & development; D014776:Virus Cultivation