A newly isolated avian sarcoma virus, ASV-1, carries the crk oncogene.
Avian sarcoma virus 1 (ASV-1) was isolated from a spontaneous sarcoma of an adult chicken. It is a replication-defective virus that induces fibrosarcomas in young chickens and oncogenic transformation in cultured chick embryo fibroblasts. The ASV-1 genome has been cloned in the lambda gt WES.lambda B vector from closed circular viral DNA extracted from infected cells. The nucleotide sequence of the oncogene insert in the ASV-1 genome has been determined. It is virtually identical to the sequence of the oncogene crk recently discovered in the avian sarcoma virus CT10. ASV-1 and CT10 are two independent retrovirus isolates carrying the crk oncogene.
Tsuchie
H
H
Norris Cancer Center, University of Southern California School of Medicine, Los Angeles 90033-1054.
Chang
C H
CH
Yoshida
M
M
Vogt
P K
PK
D000595:Amino Acid Sequence; D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D003001:Cloning, Molecular; D004270:DNA, Circular / Q000235:genetics; D005814:Genes, Viral; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D050736:Oncogene Protein v-crk; D009857:Oncogenes; D015183:Restriction Mapping; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D004270:DNA, Circular; D050736:Oncogene Protein v-crk; D015682:Retroviridae Proteins, Oncogenic