title: Determination of human antibodies to specific antigens of cytomegalovirus using an antigen capture immunoassay.
abstract: The antigen capture immunoassay which is described herein is based on the binding of specific antigens of cytomegalovirus (CMV) by monoclonal antibodies bound to a solid phase. The specificity of the binding was demonstrated by the analysis of antigens labelled with [35S]methionine and captured by the bound monoclonal antibodies. These specific antigens are recognized in turn by specific anti-cytomegalovirus antibodies in human sera. The immunoassay permits quantitation of these specific anti-cytomegalovirus antibodies and should facilitate both qualitative and quantitative comparisons of the antibodies against specific CMV antigens in different individuals.
authors.affiliation: Department of Clinical Immunology, University Hospital Groningen, The Netherlands.
authors.forename: L H
authors.initials: LH
authors.lastname: Van der Voort
authors.forename: J
authors.initials: J
authors.lastname: Van Zanten
authors.forename: L F
authors.initials: LF
authors.lastname: De Leij
authors.forename: T H
authors.initials: TH
authors.lastname: The
mesh_terms: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000276:immunology*; D003587:Cytomegalovirus / Q000276:immunology*; D006801:Humans; D007118:Immunoassay
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral