Beta-galactosidase overexpression in SV40-transformed Chinese hamster fibroblasts exposed to mutagens as a result of amplification of transfected bacterial lacZ DNA sequences.
Genetic constructions in which the bacterial lacZ gene, encoding the enzyme beta-galactosidase, is fused to a viral (SV40) origin of replication have been introduced in an SV40-transformed hamster cell line (C1102). We have studied in detail 3 clones in which beta-galactosidase-specific activity increases after treatment with genotoxic agents. We show that this increase is dependent on the activity of the viral T protein and correlates with an amplification of lac sequences. This system provides a basis for the study of the induction of gene amplification by genotoxic agents in mammalian cells.
Clément
J M
JM
Unité de Programmation Moléculaire et Toxicologie Génétique, CNRS UA271, INSERM U163, Institut Pasteur, Paris, France.
Jehanno
M
M
Hofnung
M
M
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000502:physiology; D002460:Cell Line; D002472:Cell Transformation, Viral; D004247:DNA / Q000187:drug effects / Q000235:genetics*; D005696:Galactosidases / Q000096:biosynthesis*; D005784:Gene Amplification; D007763:Lac Operon; D008322:Mammals / Q000235:genetics; D009153:Mutagens / Q000494:pharmacology*; D010957:Plasmids; D013539:Simian virus 40; D014162:Transfection; D001616:beta-Galactosidase / Q000096:biosynthesis* / Q000235:genetics
D000952:Antigens, Polyomavirus Transforming; D009153:Mutagens; D004247:DNA; D005696:Galactosidases; D001616:beta-Galactosidase