title: In vitro capacitation of goat spermatozoa.
abstract: Goat epididymal and ejaculated spermatozoa were incubated in Krebs-Ringer bicarbonate buffer containing pyruvate and lactate as energy source. A 3 hr incubation for epididymal and 4 hr for ejaculated spermatozoa was required for the capacitation and acrosome reaction to take place. Calcium is an essential requirement which was needed for motility maintenance/activation and for the initiation of acrosome reaction. A 2-fold increase in cAMP content was measured over 3 hr period of incubation of goat epididymal spermatozoa which was not seen when calcium ions were either omitted or chelated with EGTA. There is thus a definite involvement of Ca2+ ions and cAMP in capacitation and acrosome reaction of goat spermatozoa.
authors.forename: S R
authors.initials: SR
authors.lastname: Anand
authors.forename: S K
authors.initials: SK
authors.lastname: Atreja
authors.forename: M S
authors.initials: MS
authors.lastname: Chauhan
authors.forename: R
authors.initials: R
authors.lastname: Behl
mesh_terms: D000177:Acrosome / Q000502:physiology; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D000242:Cyclic AMP / Q000032:analysis; D006041:Goats / Q000502:physiology*; D008297:Male; D013075:Sperm Capacitation; D013081:Sperm Motility; D013094:Spermatozoa / Q000378:metabolism*
chemical_list: D000242:Cyclic AMP; D002118:Calcium