Tumorigenicity assay of the human mammary carcinoma cell line MCF-7.
A tumorigenicity assay was performed using DNA extracted from the human mammary carcinoma cell line MCF-7. Seven nude mouse tumors were obtained and were analyzed for the presence of human sequences and known activated oncogenes. The c-erbB.2/neu gene was identified in two tumors and two ras oncogenes in two other tumors. In order to characterize the mechanism of activation of the c-erbB.2/neu oncogene, we isolated and analyzed this gene from one of the tumors. The cloned gene did not present any rearrangement. c-erbB.2/neu transcripts from the nude mice tumors were of normal size. In one case overexpression was observed.
Marics
I
I
U. 119 INSERM, Marseille, France.
Adelaide
J
J
Rosnet
O
O
Planche
J
J
Pirisi
V
V
Birnbaum
D
D
D000818:Animals; D015139:Blotting, Southern; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D002460:Cell Line; D003001:Cloning, Molecular; D003360:Cosmids; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009693:Nucleic Acid Hybridization; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics; D011519:Proto-Oncogenes; D011988:Receptors, Thyroid Hormone; D015183:Restriction Mapping; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured / Q000166:cytology*
D004273:DNA, Neoplasm; D011518:Proto-Oncogene Proteins; D011988:Receptors, Thyroid Hormone; D011505:Protein-Tyrosine Kinases