Phospholipase D activation is functionally linked to superoxide generation in the human neutrophil.
Neutrophils stimulated with formylmethionyl-leucylphenylalanine (fMet-Leu-Phe) in the presence of butanol and ethanol formed phosphatidyl alcohols through a phospholipase D mechanism. The alcohols inhibited phosphatidic acid and diradylglycerol (DRG) formation, but did not block inositol 1, 4, 5-trisphosphate release. fMet-Leu-Phe-stimulated superoxide production was inhibited by alcohol concentrations which blocked DRG formation, whereas opsonized-zymosan-stimulated superoxide production was only partially decreased. These results suggest that phospholipase D activation is functionally linked to superoxide production in the human neutrophil.
Bonser
R W
RW
Department of Biochemical Sciences, Wellcome Research Laboratories, Beckenham, Kent, U.K.
Thompson
N T
NT
Randall
R W
RW
Garland
L G
LG
D020001:1-Butanol; D000440:Butanols / Q000494:pharmacology; D004789:Enzyme Activation; D000431:Ethanol / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology / Q000502:physiology*; D010739:Phospholipase D / Q000097:blood*; D010740:Phospholipases / Q000097:blood*; D010743:Phospholipids / Q000097:blood; D013481:Superoxides / Q000097:blood*
D000440:Butanols; D010743:Phospholipids; D013481:Superoxides; D000431:Ethanol; D009240:N-Formylmethionine Leucyl-Phenylalanine; D020001:1-Butanol; D010740:Phospholipases; D010739:Phospholipase D