Location of sites of transposon Tn916 insertion in the Mycoplasma mycoides genome.
The genome of Mycoplasma mycoides subsp. mycoides GC1176-2 was cleaved into six large fragments by the endonuclease KpnI which also cleaved the transposon Tn916 once. This has allowed genomic mapping of insertion sites for 50 transformants of GC1176-2 containing Tn916. Almost all of the mapped sites were clearly separate. The transformants provide a bank of genomes each with a KpnI site at a different position to facilitate mapping of gene loci.
Whitley
J C
JC
Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Australia.
Finch
L R
LR
D002874:Chromosome Mapping; D004251:DNA Transposable Elements; D005798:Genes, Bacterial; D015698:Genomic Library; D009176:Mycoplasma mycoides / Q000235:genetics*; D015183:Restriction Mapping
D004251:DNA Transposable Elements