Coexistence of acetylcholinesterase-, human growth hormone-releasing factor(1-37)-, alpha-melanotropin- and melanin-concentrating hormone-like immunoreactivities in neurons of the rat hypothalamus: a light and electron microscope study.
Using an antiserum (AS) raised against rat cerebral acetylcholinesterase (AChE), we revealed a neuron population in lateral and dorsal areas of the posterior rat hypothalamus. These neurons were previously described using antibodies to human growth hormone-releasing factor(1-37) (GRF-37), alpha-melanotropin (alpha-MSH) and melanin-concentrating hormone (MCH). Different intracytoplasmic distributions of the immunodeposits were observed depending on the used serum. Ultrastructural investigations demonstrated that AChE-AS labeled rough endoplasmic reticulum and nuclear envelope in control rats. MCH-AS stained Golgi apparatus in control animals and secretory granules in colchicine-injected rats. GRF-37-AS always revealed secretory granules, and alpha-MSH-AS gave the same staining only after colchicine injection.
Risold
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PY
CNRS URA 561, Laboratoire d'Histologie Embryologie Cytogénétique, Faculté de Médecine, Besançon, France.
Fellmann
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D
Lenys
D
D
Bugnon
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C
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D004721:Endoplasmic Reticulum / Q000378:metabolism / Q000648:ultrastructure; D006056:Golgi Apparatus / Q000378:metabolism / Q000648:ultrastructure; D013007:Growth Hormone-Releasing Hormone / Q000378:metabolism*; D007028:Hypothalamic Hormones; D007031:Hypothalamus / Q000378:metabolism* / Q000648:ultrastructure; D007150:Immunohistochemistry; D008543:Melanins / Q000378:metabolism*; D008854:Microscopy, Electron; D010907:Pituitary Hormones / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D000521:alpha-MSH / Q000378:metabolism*
D007028:Hypothalamic Hormones; D008543:Melanins; D010907:Pituitary Hormones; D000521:alpha-MSH; C019463:melanin-concentrating hormone; D013007:Growth Hormone-Releasing Hormone; D000110:Acetylcholinesterase