title: Mechanism of spontaneous loss of heat-stable toxin (STa) production in enterotoxigenic Escherichia coli.
abstract: Five strains of enterotoxigenic Escherichia coli (ETEC) showing spontaneous loss of heat-stable enterotoxin (STa) production were studied to elucidate the underlying genetic mechanisms. Southern blot analysis revealed that loss of STa production, and the corresponding lack of hybridization with the STa gene probes, were associated with deletions of DNA fragments harboring the relevant toxin genes rather than with loss of plasmids.
authors.affiliation: Medical Department B, University of Bergen, Haukeland Hospital, Norway.
authors.forename: H
authors.initials: H
authors.lastname: Sommerfelt
authors.forename: B I
authors.initials: BI
authors.lastname: Haukanes
authors.forename: K H
authors.initials: KH
authors.lastname: Kalland
authors.forename: A M
authors.initials: AM
authors.lastname: Svennerholm
authors.forename: J
authors.initials: J
authors.lastname: Sanchéz
authors.forename: B
authors.initials: B
authors.lastname: Bjorvatn
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics*; D015139:Blotting, Southern; D015342:DNA Probes; D004768:Enterotoxins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000472:pathogenicity; D029968:Escherichia coli Proteins; D010861:Fimbriae, Bacterial; D005798:Genes, Bacterial; D010957:Plasmids
chemical_list: D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D015342:DNA Probes; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C034151:heat stable toxin (E coli)