Amelioration of established Sendai viral pneumonia in the nude mouse using a monoclonal antibody to the virus fusion protein.
The pathological effect of parainfluenza type I (Sendai virus) is known to be a bronchopneumonia, which becomes a chronic pneumonia in the immunodeficient athymic (nude) mouse. The severity of this established chronic pneumonia can be dramatically altered by providing the nude mouse with humoral monoclonal antibodies which are neutralizing, and are directed against the fusion protein, of the virus. The alveolitis, which is a significant part of the pathology, is suppressed due to a reduction (greater than 90%) in the number of virus-infected alveolar macrophages present in the alveoli. This clearly identifies the infected alveolar macrophage as the primary effector cell in the pathogenesis of alveolitis caused by parainfluenza virus type I. The implications of using virus-neutralizing monoclonal antibodies, which have little immunomodulatory toxicity, in the treatment of viral pneumonias are discussed.
Carthew
P
P
MRC Toxicology Unit, MRC Laboratories, Carshalton, Surrey, UK.
Riley
J
J
Dinsdale
D
D
D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D002908:Chronic Disease; D005260:Female; D007124:Immunoenzyme Techniques; D008168:Lung / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D010222:Parainfluenza Virus 1, Human / Q000276:immunology*; D018184:Paramyxoviridae Infections / Q000473:pathology / Q000628:therapy*; D011650:Pulmonary Alveoli / Q000473:pathology; D014760:Viral Fusion Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D014760:Viral Fusion Proteins