Efficient immortalization by SV40 T DNA of skin fibroblasts from patients with Wilms' tumor associated with chromosome 11p deletion.
We report that transfection with a plasmid containing the SV40 early region (T) leads to a very high frequency of immortalization in two different strains of human diploid fibroblasts, each with a partial deletion of the short arm of chromosome 11. Immortalization often occurred without a recognizable "crisis" phase. Preimmortal cells showed a high frequency of spontaneous mutations and chromosomal aberrations. These results suggest that a gene involved in the control of senescence of human cells in vitro may be associated with this chromosome.
Kano
Y
Y
Department of Cancer Biology, Harvard School of Public Health, Boston, Massachusetts 02115.
Little
J B
JB
D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D002472:Cell Transformation, Viral; D002872:Chromosome Deletion; D002880:Chromosomes, Human, Pair 11; D005347:Fibroblasts; D006801:Humans; D007223:Infant; D007680:Kidney Neoplasms / Q000235:genetics*; D009154:Mutation; D013539:Simian virus 40 / Q000235:genetics*; D014162:Transfection; D009396:Wilms Tumor / Q000235:genetics*
D000952:Antigens, Polyomavirus Transforming