Activation of excitatory amino acid receptors reduces thymidine incorporation and cell proliferation rate in primary cultures of astrocytes.
Addition of quisqualate (a heterocyclic analogue of glutamate) reduced [methyl-3H]thymidine incorporation and cell proliferation in primary cultures of rat cortical astrocytes. The inhibitory action of quisqualate was mimicked by glutamate and ibotenate, whereas kainate, N-methyl-D-aspartate (NMDA) and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) were inactive. These results suggest that activation of a specific class of excitatory amino acid receptors contributes to the regulation of growth and proliferation of glial cells in primary culture.
Condorelli
D F
DF
Institute of Biochemistry, University of Catania, School of Medicine, Italy.
Ingrao
F
F
Magri
G
G
Bruno
V
V
Nicoletti
F
F
Avola
R
R
D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D001921:Brain / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D002455:Cell Division; D002478:Cells, Cultured; D005971:Glutamates / Q000494:pharmacology; D018698:Glutamic Acid; D007051:Ibotenic Acid / Q000494:pharmacology; D010069:Oxadiazoles / Q000494:pharmacology; D016318:Quisqualic Acid; D051381:Rats; D017459:Receptors, Amino Acid; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000502:physiology*; D013936:Thymidine
D005971:Glutamates; D010069:Oxadiazoles; D017459:Receptors, Amino Acid; D011956:Receptors, Cell Surface; D007051:Ibotenic Acid; D018698:Glutamic Acid; D016318:Quisqualic Acid; D013936:Thymidine