Effects of bicarbonate-containing versus lactate-containing peritoneal dialysis solutions on superoxide production by human neutrophils.
Human neutrophils were isolated from healthy volunteers and exposed to either bicarbonate-containing (pH 7.4) or lactate-containing (pH 5.2) peritoneal dialysis solution in vitro. Superoxide production by neutrophils was measured by a method based on the superoxide dismutase-inhibitable reduction of ferricytochrome c. Bicarbonate-containing peritoneal dialysis solution was found to be superior to the lactate-containing one in facilitating the production of superoxide anion by human neutrophils.
Manahan
F J
FJ
Veterans Administration Hospital, Hines, Illinois 60141.
Ing
B L
BL
Chan
J C
JC
Gupta
D K
DK
Zhou
F Q
FQ
Pal
I
I
Rahman
M A
MA
D000328:Adult; D001639:Bicarbonates / Q000494:pharmacology*; D015314:Dialysis Solutions / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007773:Lactates / Q000494:pharmacology*; D019344:Lactic Acid; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D010530:Peritoneal Dialysis; D013481:Superoxides / Q000378:metabolism*
D001639:Bicarbonates; D015314:Dialysis Solutions; D007773:Lactates; D013481:Superoxides; D019344:Lactic Acid