Changes of subcellular localization of Thy-1 antigen during thymic myoid cell differentiation.
Using a quantitative enzyme immunoassay, Thy-1 antigen expressed by a rat myoid cell line R615B2 was detected mainly on the cell surface at a single cell stage, whereas at the stage of forming myotubes, Thy-1 was found predominantly in the cytoplasm. The muscle specific creatine kinase activity also increased in association with the shift of Thy-1 from the cell surface to the cytoplasm, suggesting biological significance of Thy-1 redistribution in muscle differentiation from single cells to multinucleated cells.
Kikuchi
A
A
National Institute of Neuroscience, Tokyo, Japan.
Kamo
I
I
Fujisawa
K
K
Nonaka
I
I
D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism*; D002460:Cell Line; D007150:Immunohistochemistry; D009132:Muscles / Q000166:cytology / Q000276:immunology*; D013347:Subcellular Fractions / Q000276:immunology; D018800:Thy-1 Antigens; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology*
D000954:Antigens, Surface; D018800:Thy-1 Antigens