title: Molecular target size of an L-glutamate receptor from the cockroach central nervous system.
abstract: L-Glutamate binding to cockroach central nervous system (CNS) membranes is unaffected by freeze-drying and subsequent rehydration. The dissociation constant is similarly unaltered by irradiation at a dose of 3 Mrad which affects only the number of biologically active binding sites. A molecular weight estimate for this insect CNS L-glutamate receptor was performed using irradiation inactivation analysis. By this method a molecular target size of approximately 77.8 kDa was determined. This figure is in agreement with the molecular weight found by other authors for L-glutamate binding sites in the rat brain.
authors.affiliation: AFRC Unit of Insect Neurophysiology and Pharmacology, Department of Zoology, University of Cambridge, U.K.
authors.forename: M I
authors.initials: MI
authors.lastname: Sepúlveda
authors.forename: D B
authors.initials: DB
authors.lastname: Sattelle
mesh_terms: D000818:Animals; D003058:Cockroaches / Q000378:metabolism*; D005612:Freeze Drying; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D008297:Male; D008970:Molecular Weight; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter / Q000032:analysis* / Q000528:radiation effects
chemical_list: D005971:Glutamates; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter; D018698:Glutamic Acid