Characterization of a Mr 25,000 basic fibroblast growth factor form in adult, regenerating, and fetal rat liver.
A heparin-binding Mr 25,000 immunoreactive bFGF-like protein (ir-bFGF) is recognized in adult rat liver extract by affinity-purified polyclonal anti-human placental bFGF antibodies. Hepatic levels of this protein increase 4-fold in regenerating rat liver during the first 48 h after partial hepatectomy. Also, they appear to be higher in embryonic than in newborn or in adult rat liver. Mr 25,000 ir-bFGF from regenerating rat liver, partially purified by heparin-affinity chromatography, induces plasminogen activator activity and cell proliferation in transformed fetal bovine aortic endothelial GM 7373 cells and competes with Mr 18,000 [125I]bFGF for the binding to high affinity bFGF receptors. The data indicate the presence in rat liver of a high molecular weight form of bFGF whose expression is modulated during embryonic development and liver regeneration.
Presta
M
M
Department of Biomedical Sciences and Biotechnologies, School of Medicine, University of Brescia, Italy.
Statuto
M
M
Rusnati
M
M
Dell'Era
P
P
Ragnotti
G
G
D000375:Aging; D000818:Animals; D002417:Cattle; D002460:Cell Line; D002846:Chromatography, Affinity; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism; D005333:Fetus; D005346:Fibroblast Growth Factors / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D007106:Immune Sera; D007700:Kinetics; D008099:Liver / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D008115:Liver Regeneration; D008297:Male; D008970:Molecular Weight; D010960:Plasminogen Activators / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000378:metabolism; D017468:Receptors, Fibroblast Growth Factor; D011994:Recombinant Proteins / Q000378:metabolism; D012016:Reference Values
D007106:Immune Sera; D011956:Receptors, Cell Surface; D017468:Receptors, Fibroblast Growth Factor; D011994:Recombinant Proteins; D005346:Fibroblast Growth Factors; D010960:Plasminogen Activators