Comparison of three different activation methods for coupling antibodies to magnetisable cellulose particles.
The periodate and 1,1'-carbonyldiimidazole activation methods were compared with the cyanogen bromide procedure for coupling antibodies to magnetisable cellulose/iron oxide solid-phase particles. Fluoroimmunoassays for quinine, primaquine, normetanephrine and cannabinoids were employed to assess the binding properties of such coupled solid phases. The cyanogen bromide and 1,1'-carbonyldiimidazole methods gave similar products in most cases, while the specific binding capacity of periodate coupled particles was between two and five times lower. Nevertheless, comparable standard curves could be obtained with solid phase coupled by each method. The periodate and 1,1'-carbonyldiimidazole methods are acceptable alternatives, notably for laboratories lacking the facility to handle the toxic cyanogen bromide.
al-Abdulla
I H
IH
Department of Chemical Pathology, St. Bartholomew's Hospital, London, U.K.
Mellor
G W
GW
Childerstone
M S
MS
Sidki
A M
AM
Smith
D S
DS
D000818:Animals; D002482:Cellulose; D003488:Cyanogen Bromide / Q000494:pharmacology; D015200:Fluoroimmunoassay / Q000379:methods*; D007093:Imidazoles / Q000494:pharmacology; D008280:Magnetics; D010504:Periodic Acid / Q000494:pharmacology; D012756:Sheep
D007093:Imidazoles; D010504:Periodic Acid; C006900:N,N-carbonyldiimidazole; D002482:Cellulose; D003488:Cyanogen Bromide