Enhanced full-length transcription of Sindbis virus RNA by effective denaturation with methylmercury hydroxide.
49-S Sindbis virus RNA was reverse transcribed into a complementary DNA. The RNA templates were denatured by three different methods prior to DNA synthesis. Efficient full-length transcription was only achieved after treatment with methylmercury hydroxide.
Schelp
C
C
Institute of Virology, Hannover Veterinary School, Federal Republic of Germany.
Kaaden
O R
OR
D004247:DNA / Q000096:biosynthesis; D008767:Methylmercury Compounds / Q000494:pharmacology*; D009691:Nucleic Acid Denaturation; D012367:RNA, Viral / Q000378:metabolism*; D012845:Sindbis Virus / Q000235:genetics*; D014158:Transcription, Genetic
D008767:Methylmercury Compounds; D012367:RNA, Viral; D004247:DNA; C020771:methylmercury hydroxide