title: Mammalian tissue culture growth, viral replication, and cultivation using serum replacement factor.
abstract: Variability, cost, and availability of fetal bovine serum are in question; thus, we examined whether using a serum replacement factor in multiple mammalian tissue cultures could support not only cell growth, but also viral replication, expression, and retention of phenotypic markers. By using a serum replacement in defined media, we demonstrated multipassage cell growth of several different cell lines and viral cultivation and replication equivalent to fetal bovine serum. Moreover, by supplementing media with a serum replacement factor we observed at least a 28% financial savings.
authors.affiliation: Office of Scientific Services, Centers for Disease Control, Atlanta, Georgia 30333.
authors.forename: F J
authors.initials: FJ
authors.lastname: Candal
authors.forename: G
authors.initials: G
authors.lastname: Marchetti
authors.forename: M
authors.initials: M
authors.lastname: Davis
authors.forename: V
authors.initials: V
authors.lastname: George
authors.forename: E W
authors.initials: EW
authors.lastname: Ades
mesh_terms: D000818:Animals; D001769:Blood; D002352:Carrier Proteins; D002455:Cell Division; D002460:Cell Line; D003365:Costs and Cost Analysis; D003587:Cytomegalovirus / Q000254:growth & development; D003588:Cytopathogenic Effect, Viral; D006133:Growth Substances; D006801:Humans; D014776:Virus Cultivation / Q000379:methods*; D014779:Virus Replication
chemical_list: D002352:Carrier Proteins; D006133:Growth Substances