title: Nucleotide sequence of coronavirus TGEV genomic RNA: evidence for 3 mRNA species between the peplomer and matrix protein genes.
abstract: The region of the TGEV genome between the E1-matrix protein gene and the E2-peplomer protein gene has been sequenced from a cDNA clone. The consensus recognition sequence, 5'TTAA CTAAAC was found upstream from 3 large open reading frames. In coronaviruses these homologous recognition sequences are involved in the initiation of transcription suggesting that there are 3 mRNA species in this region of the TGEV genome. Northern blot analysis and nuclease S1 mapping confirmed the presence of 3 mRNA species between mRNA 3 encoding the E2-peplomer protein and mRNA 6 encoding the E1-matrix protein. The 5' regions of these 3 mRNAs encode potential polypeptides of predicted molecular weight; 7859, 27744 and 9287, respectively. The potential translation product of ORF B (27744 Da) is considerably larger than previously reported and could be difficult to distinguish by size from the E1-matrix protein.
authors.affiliation: USDA, Agricultural Research Service, National Animal Disease Center, Ames Iowa 50010.
authors.forename: R D
authors.initials: RD
authors.lastname: Wesley
authors.forename: A K
authors.initials: AK
authors.lastname: Cheung
authors.forename: D D
authors.initials: DD
authors.lastname: Michael
authors.forename: R D
authors.initials: RD
authors.lastname: Woods
mesh_terms: D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D003332:Coronaviridae / Q000235:genetics*; D004247:DNA / Q000032:analysis; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis*; D013552:Swine; D005760:Transmissible gastroenteritis virus / Q000235:genetics*; D014763:Viral Matrix Proteins / Q000032:analysis / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral; D014763:Viral Matrix Proteins; D004247:DNA