title: Isolation of the facA (acetyl-coenzyme A synthetase) and acuE (malate synthase) genes of Aspergillus nidulans.
abstract: Acetate inducible genes of Aspergillus nidulans were cloned via differential hybridization to cDNA probes. Using transformation of mutant strains the genes were identified as facA (acetyl-Coenzyme A synthetase) and acuE (malate synthase). The levels of RNA encoded by these genes were shown to be acetate inducible and subject to carbon catabolite repression. Induction is abolished in a facB mutant and carbon catabolite repression is relieved in a creA mutant.
authors.affiliation: Department of Genetics, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: R A
authors.initials: RA
authors.lastname: Sandeman
authors.forename: M J
authors.initials: MJ
authors.lastname: Hynes
mesh_terms: D000106:Acetate-CoA Ligase / Q000235:genetics*; D000085:Acetates / Q000494:pharmacology; D001233:Aspergillus nidulans / Q000201:enzymology / Q000235:genetics*; D015152:Blotting, Northern; D015139:Blotting, Southern; D003066:Coenzyme A Ligases / Q000235:genetics*; D004251:DNA Transposable Elements; D005786:Gene Expression Regulation; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D008292:Malate Synthase / Q000235:genetics*; D009154:Mutation; D007652:Oxo-Acid-Lyases / Q000235:genetics*; D010957:Plasmids; D012331:RNA, Fungal / Q000032:analysis; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014170:Transformation, Genetic; D014178:Translocation, Genetic
chemical_list: D000085:Acetates; D004251:DNA Transposable Elements; D012331:RNA, Fungal; D008292:Malate Synthase; D007652:Oxo-Acid-Lyases; D003066:Coenzyme A Ligases; D000106:Acetate-CoA Ligase