Chromosomal organization of the human major histocompatibility complex class I gene family.
17 HLA class I genes have been isolated from the genome of B-lymphoblastoid cell line 721. Sequence analysis and transfection studies indicate that three genes, in addition to those encoding the HLA-A, -B, and -C antigens can direct the synthesis of a class I alpha protein (4, 5, 21). Using gene-specific DNA probes to analyze the presence of restriction fragment-length polymorphisms within a large pedigree and in panel of HLA deletion mutant cell lines, we show here that two of these genes, designated HLA-G and HLA-F, are located on the short arm of chromosome 6 telomeric to the HLA-A locus. The third expressed non-A, -B, and -C class I gene, HLA-E, is located between HLA-A and HLA-C (4). In addition, the remaining 11 class I pseudogenes and gene fragments are localized relative to established markers on chromosome 6p.
Koller
B H
BH
Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis 55455.
Geraghty
D E
DE
DeMars
R
R
Duvick
L
L
Rich
S S
SS
Orr
H T
HT
D001402:B-Lymphocytes / Q000528:radiation effects; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002896:Chromosomes, Human, Pair 6; D003001:Cloning, Molecular; D008040:Genetic Linkage; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D008285:Major Histocompatibility Complex; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D014407:Tumor Cells, Cultured / Q000528:radiation effects
D015395:Histocompatibility Antigens Class I