[The role of guanidine groups of arginine residues of Na, K-ATPase and glutamate dehydrogenase in an interaction with cholesterol].
Cholesterol was studied in experiments in vitro for its effect on the activity of Na, K-ATPase of the synaptic brain membranes of rats and a crystalline preparation of glutamate dehydrogenase from the liver mitochondria of a bull. Cholesterol decreased the activity of the above enzymes. When blocking guanidine groups of arginine residues of Na, K-ATPase and glutamate dehydrogenase the inhibiting action of cholesterol was absent. The obtained data evidence for the possibility of a direct interaction of cholesterol with membrane enzymes as well as for the important significance of guanidine groups of arginine residues of proteins in the process.
Kliueva
N N
NN
Matveeva
I M
IM
Kozhevnikova
K A
KA
D000818:Animals; D001120:Arginine / Q000378:metabolism*; D001921:Brain / Q000201:enzymology; D002417:Cattle; D002784:Cholesterol / Q000378:metabolism*; D005969:Glutamate Dehydrogenase / Q000378:metabolism*; D019791:Guanidine; D006146:Guanidines / Q000378:metabolism*; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013570:Synaptic Membranes / Q000201:enzymology
D006146:Guanidines; D001120:Arginine; D002784:Cholesterol; D005969:Glutamate Dehydrogenase; D000254:Sodium-Potassium-Exchanging ATPase; D019791:Guanidine
Rol' guanidinovykh grupp argininovykh ostatkov Na, K-ATPazy i glutamatdegidrogenazy vo vzaimodeĭstvii s kholesterinom.