Renal necrosis and the involvement of a single enzyme of the de novo pathway for the biosynthesis of platelet-activating factor in the rat kidney inner medulla.
2-Bromoethylamine hydrobromide (BEA), when administered to rats, induces a highly specific papillary necrosis associated with the inner medulla. PAF levels in the blood were lowered by 50% and of the three enzymes that comprise the de novo route for PAF in the cortex/medulla, only the cholinephosphotransferase activity in the inner medulla microsomes was reduced (33%) by the BEA treatment. Moreover, BEA did not affect phosphatidylcholine synthesis in either the cortex or inner medulla. Our studies indicate that the de novo pathway for PAF synthesis in the renal inner medulla is responsible for the secretion of newly formed PAF into the blood stream and that a single enzyme in the de novo route accounts for the decreased rate of PAF synthesis during the development of renal necrosis.
Lee
T C
TC
Medical Sciences Division, Oak Ridge Associated Universities, Tennessee 37831-0117.
Malone
B
B
Woodard
D
D
Snyder
F
F
D000818:Animals; D002798:Diacylglycerol Cholinephosphotransferase / Q000378:metabolism; D005021:Ethylamines / Q000633:toxicity*; D007668:Kidney / Q000187:drug effects / Q000473:pathology*; D007672:Kidney Cortex / Q000378:metabolism; D007679:Kidney Medulla / Q000378:metabolism*; D008861:Microsomes / Q000201:enzymology; D009336:Necrosis; D010972:Platelet Activating Factor / Q000096:biosynthesis* / Q000097:blood; D051381:Rats
D005021:Ethylamines; D010972:Platelet Activating Factor; C004504:2-bromoethylamine; D002798:Diacylglycerol Cholinephosphotransferase