[Estimation of a direct regulatory effect of estrogens on hepatocytes, evaluated according to the change in the level of free estrogen-binding protein in primary cell culture].
Male rat hepatocyte cultures have been obtained. The culturing hepatocytes had a stable level of some morphological and functional properties. A high level of estrogen receptors (ER) and E2-sensitive unusual estrogen-binding protein (UEBP) was found. A direct dose-dependent inhibiting effect of physiological (10(-10)-10(-7) M) concentrations of E2 on UEBP content was established in cell cultures incubated with the hormone for 3 days. Hexestrol (but not cholesterol) was found to exert a direct inhibiting effect. The inhibiting effect of E2 (10(-9) M) was observed after a 24 hour incubation of hepatocytes with the hormone but was less pronounced. In this case a significant increase in UEBP concentration in hepatocytes was noted 72 hours after the removal of E2. It is concluded that physiological concentrations of E2 can exert a direct regulatory influence on certain functions of culturing hepatocytes acting via hepatocyte ER.
Vishniakova
T G
TG
Shnyra
A A
AA
Bocharov
A V
AV
Spirov
V G
VG
Smirnova
O V
OV
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism; D004967:Estrogens / Q000494:pharmacology*; D006589:Hexestrol / Q000494:pharmacology; D007124:Immunoenzyme Techniques; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011960:Receptors, Estrogen / Q000378:metabolism*
D002352:Carrier Proteins; D004967:Estrogens; D011960:Receptors, Estrogen; C010140:estrophilin; D006589:Hexestrol
Vyiavlenie priamogo reguliatornogo deĭstviia éstrogenov na gepatotsity, otsenivaemogo po izmeneniiu urovnia osovogo éstrogensviazyvaiushchego belka v pervichnoĭ kulture kletok.