title: [Purification of phospholipase C isoenzyme 1 from Clostridium perfringens and its properties].
abstract: A procedure for the purification of isoenzyme I of phospholipase C from Cl. perfringens was developed. The isoenzyme was purified to homogeneity (data from disc electrophoresis) using affinity chromatography on polycephamide and gel filtration through Ultrogel AcA-54, the enzyme yield being 41%. Some properties of the purified isoenzyme I (pH and temperature optima, stability, effect of metal ions and detergents, substrate dependence) were investigated. No significant differences between the properties of the unfractionated enzyme and isoenzyme I were established.
authors.forename: M M
authors.initials: MM
authors.lastname: Rakhimov
authors.forename: R A
authors.initials: RA
authors.lastname: Akhmezhanov
authors.forename: E Ia
authors.initials: EIa
authors.lastname: Sof'ina
authors.forename: F A
authors.initials: FA
authors.lastname: Sagatova
authors.forename: G F
authors.initials: GF
authors.lastname: Shemanova
mesh_terms: D002413:Cations, Divalent; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003016:Clostridium perfringens / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007527:Isoenzymes / Q000302:isolation & purification* / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism; D013501:Surface-Active Agents; D010738:Type C Phospholipases / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D002413:Cations, Divalent; D007527:Isoenzymes; D010713:Phosphatidylcholines; D013501:Surface-Active Agents; D010738:Type C Phospholipases
vernacular_title: Ochistka izofermenta 1 fosfolipazy C iz Clostridium perfringens i nekotorye ego svoĭstva.