Chromosome mapping and expression of a putative testis-determining gene in mouse.
Isolation and mapping of a mouse complementary DNA sequence (mouse Y-finger) encoding a multiple, potential zinc-binding, finger protein homologous to the candidate human testis-determining factor gene is reported. Four similar sequences were identified in Hind III-digested mouse genomic DNA. Two (7.2 and 2.0 kb) were mapped to the Y chromosome. Only the 2.0-kb fragment, however, was correlated with testis determination. Polymerase chain reaction analysis suggests both Y loci are transcribed in adult testes. A 3.6-kb fragment was mapped to the X chromosome between the T16H and T6R1 translocation breakpoints, and a fourth (6.0 kb) was mapped to chromosome 10. Hence, mYfin sequences have been duplicated several times in the mouse, although they are not duplicated in humans.
Nagamine
C M
CM
Howard Hughes Medical Institute, University of California, San Francisco 94143.
Chan
K M
KM
Kozak
C A
CA
Lau
Y F
YF
D000818:Animals; D002874:Chromosome Mapping; D004268:DNA-Binding Proteins / Q000235:genetics; D005796:Genes; D008297:Male; D008667:Metalloproteins / Q000235:genetics; D051379:Mice; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length; D012732:Sex Determination Analysis; D013737:Testis / Q000033:anatomy & histology*; D014158:Transcription, Genetic; D014960:X Chromosome; D014998:Y Chromosome
D004268:DNA-Binding Proteins; D008667:Metalloproteins