title: Characterization of Pseudomonas mercury-resistance transposon Tn502, which has a preferred insertion site in RP1.
abstract: Tn502mer differs in size and restriction map from the well-characterized Tn501mer. It also differs in its preferential and high-frequency insertion into the 6 kb PstI-C region of RP1. The affinity for this region is perpetuated in pVS76, a clone of RP1 PstI-C in pBR322. Restriction mapping of independent pVS76::Tn502 derivatives revealed that Tn502 inserted at the same site (or small region) in PstI-C corresponding to the 35 kb coordinate in RP1. Insertion occurred in both orientations, but one was preferred. When PstI-C was deleted from RP1, acquisition of Tn502 was reduced and the sites of insertion randomized.
authors.affiliation: Department of Microbiology, La Trobe University, Bundoora, Australia.
authors.forename: V A
authors.initials: VA
authors.lastname: Stanisich
authors.forename: R
authors.initials: R
authors.lastname: Arwas
authors.forename: P M
authors.initials: PM
authors.lastname: Bennett
authors.forename: F
authors.initials: F
authors.lastname: de la Cruz
mesh_terms: D001665:Binding Sites; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D004352:Drug Resistance, Microbial / Q000235:genetics; D008628:Mercury / Q000494:pharmacology*; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics*; D015183:Restriction Mapping
chemical_list: D004251:DNA Transposable Elements; D008628:Mercury