title: Expression of virally transduced mouse tyrosinase in tyrosinase-negative chick embryo melanocytes in culture.
abstract: A cDNA encoding mouse tyrosinase was inserted into a plasmid containing the provirus of a replication competent avian leukosis virus (ALV). A viral stock produced from the plasmid was used to infect cultured tyrosinase-negative (ca/ca) unpigmented chick embryo melanocytes. Five days after infection many cells were producing very dark discrete melanosomes.
authors.affiliation: School of Biological Sciences, University of Nebraska, Lincoln 68588-0118.
authors.forename: B A
authors.initials: BA
authors.lastname: Whitaker
authors.forename: T J
authors.initials: TJ
authors.lastname: Frew
authors.forename: J J
authors.initials: JJ
authors.lastname: Greenhouse
authors.forename: S H
authors.initials: SH
authors.lastname: Hughes
authors.forename: H
authors.initials: H
authors.lastname: Yamamoto
authors.forename: T
authors.initials: T
authors.lastname: Takeuchi
authors.forename: J A
authors.initials: JA
authors.lastname: Brumbaugh
mesh_terms: D000818:Animals; D001354:Avian Leukosis Virus / Q000235:genetics; D004156:Catechol Oxidase / Q000378:metabolism*; D002478:Cells, Cultured; D002642:Chick Embryo; D015870:Gene Expression; D008544:Melanocytes / Q000201:enzymology*; D051379:Mice; D014442:Monophenol Monooxygenase / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids; D014161:Transduction, Genetic
chemical_list: D004156:Catechol Oxidase; D014442:Monophenol Monooxygenase