Analysis of foot-and-mouth disease virus-neutralizing idiotypes from immune bovine and swine with anti-murine idiotype antibody probes.
Rabbit anti-idiotypic antibodies (a-IdAb) induced by foot-and-mouth disease virus (FMDV) neutralizing mAb were used as probes to identify anti-FMDV Id in immune serum from bovine and swine. In a competitive RIA, at least two of the a-IdAb exhibited a dose-dependent capacity to compete with labeled virus for anti-FMDV antibodies from a convalescent bovine serum. These a-IdAb were immobilized on activated Sepharose and used to isolate anti-viral Id from bovine, swine, and murine FMDV immune sera. Both the bovine and swine antibodies recovered from the a-IdAb/Sepharose columns reacted with virus, and to a lesser extent with corresponding mAb-resistant virus variants. The binding of affinity isolated bovine and swine antibodies to virus was specifically inhibited by the homologous a-IdAb, and in addition, both were capable of neutralizing FMDV in suckling mouse protection and plaque reduction neutralization assays. Therefore, by means of a-IdAb probes generated against FMDV murine Id, two neutralizing Id were identified in bovine and swine. These results suggest that FMDV-neutralizing epitopes recognized by murine systems play a role in the overall immunity of foot-and-mouth disease-susceptible animals.
Garmendia
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Molecular Biology Laboratory, United States Department of Agriculture, Plum Island Animal Disease Center, Greenport, NY.
Borca
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MV
Morgan
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Baxt
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B
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D005537:Aphthovirus / Q000276:immunology*; D002417:Cattle / Q000276:immunology*; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D051379:Mice; D009500:Neutralization Tests; D013552:Swine / Q000276:immunology*; D010948:Viral Plaque Assay; D015678:Viral Structural Proteins / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D000914:Antibodies, Viral; D000956:Antigens, Viral; D007130:Immunoglobulin Idiotypes; D015678:Viral Structural Proteins