DOT-enzyme linked immunosorbent assay for detection of Clostridium perfringens type A enterotoxin.
A procedure, which we have termed DOT-ELISA, to detect Clostridium perfringens type A enterotoxin on nitrocellulose paper is described. Seventy eight preparations from 39 cultures of C. perfringens type A were tested simultaneously by this and by Plate-ELISA methods. The results were comparable. DOT-ELISA detected as little as 0.02 micrograms of purified enterotoxin and 0.13 micrograms of enterotoxin in cell-free culture supernatant. As little as 0.02 micrograms purified enterotoxin mixed with human faeces could be detected specifically. The method is simple and does not require an ELISA reader.
Mehta
R
R
Ranchi Veterinary College, Birsa Agricultural University, India.
Narayan
K G
KG
Notermans
S
S
D003016:Clostridium perfringens; D004768:Enterotoxins / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000032:analysis; D006801:Humans
D004768:Enterotoxins; C035598:enterotoxin, Clostridium