title: DNA nucleotide sequence analysis of the immediate-early gene of pseudorabies virus.
abstract: The complete DNA sequence coding for the immediate-early protein (IE180) of pseudorabies virus was determined. The coding region of IE180 is 4380 nucleotides for 1460 amino acid residues. G+C content of the non-coding portion of the IE gene is 70.3% while the G+C content of the coding portion is considerably higher at 80.1%. Correspondingly, codons consisting mainly of Gs and Cs are favoured. Clusters of amino acid homologies are observed among IE180 of pseudorabies virus, ICP4 of herpes simplex virus type-1 and IE140 of varicella-zoster virus, and are organized similarly in all three polypeptides. Functions exhibited by IE180 are assigned, tentatively, to structural domains of the molecule by analogy to the HSV-1 ICP4 polypeptide.
authors.affiliation: USDA, National Animal Disease Center, Ames, IA 50010.
authors.forename: A K
authors.initials: AK
authors.lastname: Cheung
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000302:isolation & purification; D001482:Base Composition; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D005814:Genes, Viral; D011558:Herpesvirus 1, Suid / Q000235:genetics*; D014645:Herpesvirus 3, Human / Q000235:genetics; D017874:Immediate-Early Proteins; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D018139:Simplexvirus / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D000596:Amino Acids; D004279:DNA, Viral; D017874:Immediate-Early Proteins; D014764:Viral Proteins; C047583:pseudorabies virus immediate early proteins