title: Efficient medium for impingement and storage of enveloped viruses.
abstract: Airborne infections with pathogenic viruses play an important role in the transmission of diseases amongst men and animals. We compared several media intended for impingement of viruses from virus-contaminated air and for their preserving effect for two enveloped viruses. Sindbis (SINV) and vesicular stomatitis virus (VSV), members of the families Toga- and Rhabdoviridae, respectively, were chosen as indicator agents. Amongst the media tested, a sampling fluid consisting of phosphate buffered saline, pH 7.2, 0.5% bovine serum albumin, 0.5% gelatine (PBSplus) was most efficient to minimize the sampling stress during impingement and to preserve the infectivity SINV and VSV under stringent conditions at 37 degrees C. About 50% of virus infectivity was recovered 15.7 or 30 hours, respectively, after the beginning of storage. Thus the recommended medium is also suitable for shipment and storage of diagnostic virus samples.
authors.forename: B
authors.initials: B
authors.lastname: Stolze
authors.forename: O R
authors.initials: OR
authors.lastname: Kaaden
mesh_terms: D000391:Air Microbiology; D000818:Animals; D002460:Cell Line; D003470:Culture Media; D007700:Kinetics; D012044:Regression Analysis; D012845:Sindbis Virus / Q000254:growth & development*; D014721:Vesicular stomatitis Indiana virus / Q000254:growth & development*
chemical_list: D003470:Culture Media