Heterogeneous distribution of nuclear triiodothyronine receptors in liver and preadipose cells as evidenced by their reactivity to antibodies against different protein products of erb A oncogenes.
Polyclonal antibodies raised in rabbits against bacterially produced peptides in the C-terminal region of v-erb A or human c-erb A alpha oncogenes recognize the nuclear triiodothyronine (T3) receptors in the T3-sensitive Ob 17 mouse preadipocyte cell line and not in mouse or rat liver. The results confirm the existence of different T3 receptors in different tissues. The results also suggest a heterogeneous receptor distribution within the preadipose cell line, with a predominance of c-erb A alpha-type species. Antibodies raised against domain 149 227, but not against domain 245-325, impair T3 binding, suggesting a role for this domain in ligand binding.
Torresani
J
J
INSERM U 38, CNRS URA 178, Laboratoire de Biochimie Médicale, Faculté de Médecine, Marseille, France.
Ghysdael
J
J
Bismuth
J
J
Teboul
M
M
Ghiringhelli
O
O
Savary
J
J
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D002850:Chromatography, Gel; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice; D011518:Proto-Oncogene Proteins / Q000276:immunology*; D011519:Proto-Oncogenes; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D011988:Receptors, Thyroid Hormone / Q000276:immunology / Q000378:metabolism*; D014284:Triiodothyronine / Q000276:immunology / Q000378:metabolism*
D000906:Antibodies; D011518:Proto-Oncogene Proteins; D011988:Receptors, Thyroid Hormone; D014284:Triiodothyronine