Detection of human papillomavirus DNA in cervical cancer tissue by the polymerase chain reaction.
A method for detecting HPV DNA in cervical cancer tissue was developed without using isotopes. The DNA samples from the cancer tissues were first subjected to amplification by PCR, followed by polyacrylamide gel electrophoresis to identify the specific amplified fragment. The specificity and sensitivity of the PCR method are described. Compared with the dot hybridization technique, it is shown that the method is able to detect HPV DNA in cervical cancer tissues.
He
Y K
YK
Department of Biochemistry, First Medical College of PLA, People's Republic of China.
Zhang
J Z
JZ
Xu
Q A
QA
Gao
J M
JM
D001483:Base Sequence; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D005069:Evaluation Studies as Topic; D005260:Female; D006367:HeLa Cells / Q000032:analysis; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000032:analysis*
D015344:DNA Probes, HPV; D004279:DNA, Viral