[Establishment and studies of two human pancreatic carcinoma in vivo lines by transplanting the tumor into nude mice].
Two human pancreatic adenocarcinoma in vivo lines (PCT-1, PCT-2) were established in athymic nude mice and maintained for 27 passages (PCT-1) and 7 passages (PCT-2) respectively in 29 (PCT-1) and 10.5 months (PCT-2). Tumours obtained from metastatic lymph node (PCT-1) and metastatic nodule in the omentum (PCT-2) were the initial sources for implantation. All xenografts in the two tumour lines were studied by using histological, ultrastructural, immunohistochemical and cytogenetical methods. PCT-1 maintained the poorly differentiated adenocarcinoma features with little mucin production, and CEA was weakly positive with metastasis to lymph nodes in this tumour line. PCT-2 was a moderately differentiated adenocarcinoma line with abundant mucin secretion, CEA was strongly positive. The poorly differentiated tumours have a faster growth rate in comparison with those of the moderately and well differentiated ones.
Chen
J
J
D000230:Adenocarcinoma / Q000473:pathology*; D002288:Adenocarcinoma, Mucinous / Q000473:pathology*; D000818:Animals; D002460:Cell Line; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D009368:Neoplasm Transplantation; D010190:Pancreatic Neoplasms / Q000473:pathology*