title: The binding of 99mTc(Sn)-MDP complexes to human serum albumin and other blood proteins determined with gel chromatography and ultrafiltration.
abstract: The binding of 99mTc(Sn)-MDP to human serum albumin and other blood proteins was investigated by gel chromatography and ultrafiltration. During gel chromatography dissociation of the 99mTc(Sn)-MDP-protein complex occurs: thus, it is not a suitable technique for the determination of protein binding. The values found with ultrafiltration have to be corrected for non-ultrafiltrable TcO2.nH2O. From the corrected values it can be concluded that binding of 99mTc(Sn)-MDP to blood proteins does not play a role in the biodistribution.
authors.affiliation: Analytical Chemistry Laboratory, State University of Utrecht, The Netherlands.
authors.forename: Y M
authors.initials: YM
authors.lastname: Huigen
authors.forename: T G
authors.initials: TG
authors.lastname: Tji
authors.forename: W J
authors.initials: WJ
authors.lastname: Gelsema
authors.forename: C L
authors.initials: CL
authors.lastname: de Ligny
mesh_terms: D001798:Blood Proteins / Q000378:metabolism*; D002850:Chromatography, Gel; D006801:Humans; D012709:Serum Albumin / Q000378:metabolism*; D013669:Technetium Tc 99m Medronate / Q000378:metabolism*; D014462:Ultrafiltration
chemical_list: D001798:Blood Proteins; D012709:Serum Albumin; C023568:technetium Tc 99m (Sn)methylenediphosphonate; D013669:Technetium Tc 99m Medronate