title: Assignment of common allele loss in osteosarcoma to the subregion 17p13.
abstract: Human osteosarcomas frequently show loss of alleles on chromosome 17 as well as those on chromosome 13 that harbors the retinoblastoma gene, indicating concerted operation of another tumor-suppressing gene on chromosome 17. To assign the affected gene to a defined region of chromosome 17, we performed mitotic recombination/deletion mapping by the use of 10 polymorphic loci on chromosome 17. Of 37 tumors studied, 28 (75.7%) showed loss of heterozygosity on chromosome 17. The affected regions varied among tumors, ranging in extent from a whole chromosome to a distal segment of the short arm. However, allele loss in one region, notably in 17p13 between D17S1 and D17S30, was common to all 28 tumors, suggesting the presence of a tumor-suppressing gene in this defined region.
authors.affiliation: Radiation Biology Center, Faculty of Medicine, Kyoto University, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Toguchida
authors.forename: K
authors.initials: K
authors.lastname: Ishizaki
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: M S
authors.initials: MS
authors.lastname: Sasaki
authors.forename: M
authors.initials: M
authors.lastname: Ikenaga
authors.forename: M
authors.initials: M
authors.lastname: Kato
authors.forename: M
authors.initials: M
authors.lastname: Sugimoto
authors.forename: Y
authors.initials: Y
authors.lastname: Kotoura
authors.forename: T
authors.initials: T
authors.lastname: Yamamuro
mesh_terms: D000483:Alleles; D015139:Blotting, Southern; D002872:Chromosome Deletion; D002886:Chromosomes, Human, Pair 17; D004273:DNA, Neoplasm / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D015252:Deoxyribonucleases, Type II Site-Specific; D006801:Humans; D007962:Leukocytes / Q000166:cytology; D009362:Neoplasm Metastasis; D012516:Osteosarcoma / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping
chemical_list: D004273:DNA, Neoplasm; D015252:Deoxyribonucleases, Type II Site-Specific; C027046:TCGA-specific type II deoxyribonucleases