GPC phosphodiesterase and phosphomonoesterase activities of renal cortex and medulla of control, antidiuresis and diuresis rats.
Glycerylphosphorylcholine (GPC) concentration was reported to be elevated in renal medulla of experimental animals deprived of water. The activities of GPC phosphodiesterases were similar in homogenates and membrane subfractions of renal cortex prepared from control, diuresis and antidiuresis rats. There were no differences in these preparations' ability to hydrolyze phosphorylcholine. In contrast, there was a nearly 50% reduction of non-specific phosphomonoesterase activity, using p-nitrophenylphosphate as substrate and membrane subfractions prepared from the antidiuresis animals. It is suggested that as a consequence, a pathway for the formation from L-alpha-glycerylphosphate is activated.
Kanfer
J N
JN
Department of Biochemistry and Molecular Biology, University of Manitoba, Winnipeg, Canada.
McCartney
D G
DG
D000818:Animals; D004231:Diuresis; D005997:Glycerylphosphorylcholine / Q000378:metabolism*; D007672:Kidney Cortex / Q000201:enzymology*; D007679:Kidney Medulla / Q000201:enzymology*; D009596:Nitrophenols / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D010767:Phosphorylcholine / Q000378:metabolism; D051381:Rats; D013395:Sucrose / Q000494:pharmacology
D009596:Nitrophenols; D009943:Organophosphorus Compounds; D010767:Phosphorylcholine; C008644:nitrophenylphosphate; D013395:Sucrose; D005997:Glycerylphosphorylcholine; D010727:Phosphoric Diester Hydrolases; C020677:glycerophosphocholine phosphodiesterase; C061931:glycerophosphocholine cholinephosphodiesterase