FH3, a v-myc avian retrovirus with limited transforming ability.
We have isolated a new acute avian transforming virus which contains the oncogene myc. This virus, designated FH3, was isolated after injection of a 10-day-old chick embryo with avian leukosis virus. While FH3 shares many properties with other v-myc-containing avian retroviruses, it also has several unique properties. The primary target for transformation in vitro is chicken macrophages; infection of chicken fibroblasts does not lead to complete morphological transformation. FH3 also exhibits a limited host range, in that Japanese quail macrophages and fibroblasts are infected but are not completely transformed. FH3 induces in vivo a limited tumor type if injected into 10-day-old chick embryos; only a cranial myelocytoma, which does not appear to be metastatic, can be detected. The v-myc gene of FH3 is expressed predominantly as a P145 Gag-Myc protein which is encoded by a ca. 8-kilobase genomic RNA. This FH3-encoded polyprotein is localized in the nucleus of all infected cells, whether or not they are transformed.
Chen
C
C
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
Biegalke
B J
BJ
Eisenman
R N
RN
Linial
M L
ML
D000818:Animals; D001353:Avian Leukosis / Q000382:microbiology / Q000473:pathology; D001354:Avian Leukosis Virus / Q000235:genetics*; D001483:Base Sequence; D002455:Cell Division; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D005814:Genes, Viral; D008969:Molecular Sequence Data; D016274:Oncogene Protein p55(v-myc); D009857:Oncogenes; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D015183:Restriction Mapping; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics*; D014771:Virion / Q000235:genetics
D016274:Oncogene Protein p55(v-myc); D015682:Retroviridae Proteins, Oncogenic; D011505:Protein-Tyrosine Kinases