title: Experimental aspects of isolation of myocytes from adult guinea-pig hearts.
abstract: Ionic channels can now be effectively studied on enzymatically isolated cardiac myocytes by means of the patch clamp technique. Three procedures reported to give consistently high yields of Ca-tolerant myocytes were tested for applicability to calcium channel studies under our laboratory conditions. None of them was found to be suitable for direct use. Therefore, a modified method for isolation of myocytes from adult guinea-pig hearts was developed. Calcium channel currents measured in Ca-tolerant myocytes isolated by this procedure have been presented and problems of myocytes isolation and of patch-clamp measurement discussed.
authors.affiliation: Centre of Physiological Sciences, Slovak Academy of Sciences, Bratislava.
authors.forename: I
authors.initials: I
authors.lastname: Zahradník
authors.forename: A
authors.initials: A
authors.lastname: Zahradníková
mesh_terms: D000818:Animals; D002118:Calcium; D015220:Calcium Channels / Q000032:analysis; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D005260:Female; D006168:Guinea Pigs; D008297:Male; D003012:Microbial Collagenase; D009206:Myocardium / Q000166:cytology*; D010477:Perfusion / Q000379:methods
chemical_list: D015220:Calcium Channels; D003012:Microbial Collagenase; D002118:Calcium