Red plaque formation of Coxiella burnetii and reduction assay by monoclonal antibodies.
A red plaque technique for C. burnetii which utilizes primary chicken embryo cells, is described. Red plaques could be consistently detected as early as 6 days, usually 8 days post inoculation (p.i.), reflecting that C. burnetii proliferated within the phagolysosomes of host cells. Incubation with phase II monoclonal antibodies or inactivated immune sera containing phase I and phase II antibodies or phase II antibodies only, markedly reduced phase II C. burnetii red plaques. On the other hand, red plaques from phase I organisms increased several times when phase I cells were mixed with phase I monoclonal antibodies or inactivated immune sera containing phase I and phase II antibodies. By indirect red plaque reduction assay red plaque production by phase II cells could be reduced as well.
Cheng
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Department of Microbiology, Third Military Medical College, Chongqing Sichuan province, People's Republic of China.
Yu
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SR
Yu
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GQ
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000502:physiology; D002642:Chick Embryo; D003381:Coxiella / Q000254:growth & development*; D003588:Cytopathogenic Effect, Viral; D010948:Viral Plaque Assay
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral