title: Volume-regulatory K+ fluxes in the isolated perfused rat liver: characterization by ion transport inhibitors.
abstract: Net hepatic release and uptake of K+ were examined in isolated perfused rat livers subjected to a 10-min period of hypotonic stress. Effluent Na+, K+, and Ca2+ activities were monitored throughout. Initiation and termination of hypotonic stress triggered sharp transient (less than 1 min) changes in effluent ion activities that indicated net water movement into and out of the liver, respectively. In addition, hypotonic stress caused a large transient net release of hepatic K+, whereas return to isotonicity triggered a transient net hepatic K+ uptake. The hypotonically induced K+ release was inhibited by 2 mM barium (95%) and by 1 mM quinine (60%). Net K+ influx, on the other hand, was inhibited by 1 mM ouabain (100%) and by 1 mM amiloride (50%). Osmotically induced K+ fluxes were not significantly affected by bicarbonate removal and were only partially inhibited by 0.1 mM 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS) or bumetanide. The results suggest that K+ conductance increases during hypotonic stress, whereas return to isotonicity induces a ouabain-sensitive K+ uptake partly because of increased Na+-H+ exchange. These mechanisms probably participate in regulatory volume decrease and regulatory volume increase, respectively.
authors.affiliation: Department of General and Experimental Pathology, University of Vienna, Austria.
authors.forename: P
authors.initials: P
authors.lastname: Haddad
authors.forename: J
authors.initials: J
authors.lastname: Graf
mesh_terms: D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D001464:Barium / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D002352:Carrier Proteins / Q000502:physiology; D002463:Cell Membrane Permeability; D006982:Hypertonic Solutions; D007477:Ions / Q000493:pharmacokinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D009994:Osmolar Concentration; D010042:Ouabain / Q000494:pharmacology; D010477:Perfusion; D011188:Potassium / Q000378:metabolism* / Q000493:pharmacokinetics; D011803:Quinine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D017923:Sodium-Hydrogen Exchangers
chemical_list: D002352:Carrier Proteins; D006982:Hypertonic Solutions; D007477:Ions; D017923:Sodium-Hydrogen Exchangers; D001464:Barium; D010042:Ouabain; D000584:Amiloride; D011803:Quinine; D011188:Potassium