Preparation and biochemical characterization of Lassa virus subvirion fractions.
14C-labelled Lassa virus was purified by isopycnic centrifugation and split to subvirion fractions. The purified virus was treated with nonionic detergents Nonidet P-40 (NP-40) and with octylglycoside. After ultracentrifugation in urographin density gradient, two subvirion fractions with buoyant density of 1.24-1.26 and 1.08-1.10 g/cm3 were obtained. The first fraction corresponded to the nucleocapsid of Lassa virus: it contained a protein with molecular mass of 60 kDa, the L and S segments of the genomic RNA. The second one contained a protein with molecular mass of 48 kDa and represented, apparently, envelope fraction of virus particles.
Lemeshko
N N
NN
Byelorussian Research Institute of Epidemiology and Microbiology, Minsk, U.S.S.R.
Gvelesjani
I O
IO
Ilkevich
J S
JS
Lukashevicp
I L
IL
D001116:Arenaviridae / Q000032:analysis*; D002499:Centrifugation, Density Gradient; D007836:Lassa virus / Q000032:analysis* / Q000648:ultrastructure; D012367:RNA, Viral / Q000302:isolation & purification; D014764:Viral Proteins / Q000302:isolation & purification; D014771:Virion / Q000032:analysis
D012367:RNA, Viral; D014764:Viral Proteins