Positive autoregulation of the myogenic determination gene MyoD1.
Transfection of cDNA expression vectors encoding either MyoD1 or myogenin into 10T1/2 cells converts them to myogenic cells. We show that transfection of 10T1/2 cells with the MyoD1 cDNA activates expression of endogenous MyoD1 mRNA, indicating that MyoD1 is subject to positive autoregulation. This activation of endogenous MyoD1 mRNA was also observed in Swiss 3T6 cells, but not in several other fibroblast or adipoblast cell lines transfected with the MyoD1 cDNA. In addition, transfection of the MyoD1 cDNA leads to activation of myogenin expression, and transfection of the myogenin cDNA leads to activation of MyoD1 expression. Thus, MyoD1 and myogenin appear to function in a positive autoregulatory loop that could either: account for or contribute to the stability of myogenic commitment; or amplify the level of expression of both MyoD1 and myogenin above a critical threshold that is required for activation of the myogenic program.
Thayer
M J
MJ
Department of Genetics, Hutchinson Cancer Research Center, Seattle, Washington 98104.
Tapscott
S J
SJ
Davis
R L
RL
Wright
W E
WE
Lassar
A B
AB
Weintraub
H
H
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005786:Gene Expression Regulation; D005809:Genes, Regulator; D005822:Genetic Vectors; D006706:Homeostasis; D051379:Mice; D009124:Muscle Proteins / Q000235:genetics*; D009132:Muscles / Q000166:cytology* / Q000378:metabolism; D018008:Myogenin; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014162:Transfection
D009124:Muscle Proteins; C503801:Myog protein, mouse; D018008:Myogenin; D012333:RNA, Messenger