Differentiation of germ cells in seminiferous tubules transplanted to testes of germ cell-deficient mice of W/Wv and Sl/Sld genotypes.
(WB X C57BL/6)F1-W/Wv (hereafter, WBB6F1-W/Wv) mice and (WC X C57BL/6)F1-Sl/Sld (hereafter, WCB6F1-Sl/Sld) mice are sterile due to the deficient spermatogenesis in the testes. The cause of deficient spermatogenesis in WBB6F1-W/Wv mice is considered to be a defect in germ cells themselves, whereas that in WCB6F1-Sl/Sld mice is considered to be a defect in tissue environment necessary for differentiation of germ cells. Seminiferous tubules isolated from cryptorchid testes of C57BL/6- +/+ mice were transplanted into the testes of WBB6F1-W/Wv and WCB6F1-Sl/Sld mice to clarify that the extratubular environment of these mice was intact or not. Type A spermatogonia in the transplanted tubules normally differentiated into spermatids, suggesting that the extratubular environment is intact in both WBB6F1-W/Wv and WCB6F1-Sl/Sld mice.
Kuroda
H
H
Department of Pathology, Osaka University Medical School, Japan.
Nakayama
H
H
Namiki
M
M
Matsumoto
K
K
Nishimune
Y
Y
Kitamura
Y
Y
D000818:Animals; D002454:Cell Differentiation; D003456:Cryptorchidism / Q000473:pathology*; D007248:Infertility, Male / Q000235:genetics / Q000473:pathology*; D007985:Leydig Cells / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012671:Seminiferous Tubules / Q000166:cytology* / Q000502:physiology / Q000637:transplantation; D013093:Spermatogonia / Q000166:cytology*; D013094:Spermatozoa / Q000166:cytology*; D013737:Testis / Q000166:cytology* / Q000473:pathology / Q000601:surgery