The role of cyclin synthesis and degradation in the control of maturation promoting factor activity.
We show that cyclin plays a pivotal role in the control of mitosis. A proteolysis-resistant mutant of cyclin prevents the inactivation of maturation promoting factor and the exit from mitosis both in vivo and in vitro. We have used a fractionated extract to study the activation of MPF by added cyclin protein.
Murray
A W
AW
Department of Biochemistry and Biophysics, School of Medicine, University of California, San Francisco 94143-0448.
Solomon
M J
MJ
Kirschner
M W
MW
D000818:Animals; D002453:Cell Cycle; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000502:physiology; D005260:Female; D006133:Growth Substances / Q000378:metabolism*; D007700:Kinetics; D016200:Maturation-Promoting Factor; D008540:Meiosis; D008938:Mitosis; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000378:metabolism*; D010063:Ovum / Q000166:cytology; D018809:Proliferating Cell Nuclear Antigen; D014981:Xenopus
D006133:Growth Substances; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; D016200:Maturation-Promoting Factor