The cellular 107K protein that binds to adenovirus E1A also associates with the large T antigens of SV40 and JC virus.
The association between the retinoblastoma protein (p105-RB) and either the large T antigen of SV40 or the E1A proteins of adenovirus is thought to be an important step in transformation by these viral oncogenes. E1A and large T antigen share a small region of amino acid homology that is necessary for high affinity binding with p105-RB. Mutations of this homology region were shown to reduce drastically the frequency of transformation mediated by the E1A or large T oncogenes. Previously, this small region in E1A was shown to be sufficient for interaction with a second cellular protein of 107,000 daltons (107K). Here we show that in human cells, the large T antigens of SV40 or JC virus also form complexes with 107K. Demonstration of complexes between 107K and the large T antigens of SV40 and JC virus suggests that these associations may represent another component of a common mechanism for transformation between adenoviruses and polyoma viruses.
Dyson
N
N
Cold Spring Harbor Laboratory, New York 11724.
Buchkovich
K
K
Whyte
P
P
Harlow
E
E
D017456:Adenovirus Early Proteins; D000952:Antigens, Polyomavirus Transforming / Q000276:immunology*; D000957:Antigens, Viral, Tumor / Q000276:immunology*; D002461:Cell Line, Transformed; D005786:Gene Expression Regulation; D006801:Humans; D007577:JC Virus / Q000276:immunology*; D009856:Oncogene Proteins, Viral / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism; D011120:Polyomavirus / Q000276:immunology*; D011506:Proteins / Q000276:immunology / Q000378:metabolism*; D012175:Retinoblastoma / Q000235:genetics / Q000378:metabolism; D016160:Retinoblastoma Protein; D013539:Simian virus 40 / Q000276:immunology*
D017456:Adenovirus Early Proteins; D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor; D009856:Oncogene Proteins, Viral; D010750:Phosphoproteins; D011506:Proteins; D016160:Retinoblastoma Protein