Replication of latent Epstein-Barr virus genomes in normal and malignant lymphoid cells.
DNA replication of 2 human lymphoid cell lines (U296 and Raji), latently infected with the Epstein-Barr virus, has been compared using a density transfer approach. Typical of non-malignant lymphoblastoid cells, U296 cells divided once in bromodeoxyuridine-supplemented medium to form hybrid but not heavy-density host DNA. Replication of the intracellular Epstein-Barr virus DNA was selectively inhibited in these cells with only 15% of the viral genomes duplicating once to form hybrid-density viral DNA. However, some heavy-density viral DNA was formed in the U296 cells and DNA synthesis can thus initiate again on newly duplicated viral genomes in cells that have traversed only a single S phase. These results contrast strongly with observations concerning the Burkitt-lymphoma-derived cell line. Lymphoma cells are not growth-inhibited and most of the latent Epstein-Barr virus genomes of the Raji line replicated once, and only once, in successive S phases. While the majority of the 50 Epstein-Barr virus genomes of both the Raji and U296 cell lines are maintained as extra-chromosomal DNA plasmids, the control of their duplication is distinctly different in the respective malignant and non-malignant host cells.
Adams
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Department of Medical Microbiology and Immunology, University of Minnesota, Duluth 55812.
Pozos
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TC
Purvey
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HV
D000956:Antigens, Viral / Q000378:metabolism; D001973:Bromodeoxyuridine / Q000378:metabolism; D002460:Cell Line; D004261:DNA Replication; D019309:Epstein-Barr Virus Nuclear Antigens; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D008223:Lymphoma / Q000382:microbiology*; D014779:Virus Replication
D000956:Antigens, Viral; D019309:Epstein-Barr Virus Nuclear Antigens; D001973:Bromodeoxyuridine