The effects of dipyridamole and indomethacin on methotrexate cytotoxicity in LoVo human colon cancer cells.
Dipyridamole and indomethacin were studied for their effects in the in-vitro response of LoVo colon cancer cells to methotrexate (MTX) using a dye elution method. Dipyridamole 0.5-5 micrograms mL-1 or indomethacin 1 microgram mL-1 alone had little or no effect on cell growth. The tumour cells were refractory to even high concentrations of MTX (2.5-10 micrograms mL-1) alone or with indomethacin 1 microgram mL-1. In contrast, dipyridamole 0.5-5 micrograms mL-1 sensitized the cells to MTX 5 micrograms mL-1 (their growth was reduced by 25 to 69%), possibly by inhibiting thymidine salvage.
Gaffen
J D
JD
Department of Surgery, King's College School of Medicine and Dentistry, London, UK.
Chambers
E A
EA
Bennett
A
A
D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003110:Colonic Neoplasms / Q000473:pathology*; D004176:Dipyridamole / Q000494:pharmacology*; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology*; D008727:Methotrexate / Q000633:toxicity*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology
D004176:Dipyridamole; D007213:Indomethacin; D008727:Methotrexate