Existence of a polar digitalis-like factor in mammalian hypothalamus.
We were able to partially purify a polar digitalis-like factor from rat and bovine hypothalami based on the capacity to inhibit [3H]ouabain binding to intact human erythrocytes. This factor was characterized in reference to the digitalis-like factor that we have isolated and reported on. Hypothalamic factor shared digitalis-like activities and physicochemical properties with the one derived from human urine and mammalian plasma. These findings strongly suggest that a polar digitalis-like factor identical to the circulatory factor does exist in mammalian hypothalamus.
Goto
A
A
Second Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Yamada
K
K
Ishii
M
M
Yoshioka
M
M
Ishiguro
T
T
Eguchi
C
C
Sugimoto
T
T
D000818:Animals; D001667:Binding, Competitive; D001798:Blood Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D002298:Cardenolides; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004077:Digoxin; D004285:Dogs; D004912:Erythrocytes / Q000378:metabolism; D006801:Humans; D007031:Hypothalamus / Q000502:physiology*; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D010042:Ouabain / Q000097:blood; D051381:Rats; D012413:Rubidium / Q000378:metabolism; D012503:Saponins; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*
D001798:Blood Proteins; D002298:Cardenolides; D012503:Saponins; C034504:digoxin-like factors; D010042:Ouabain; D004077:Digoxin; D000254:Sodium-Potassium-Exchanging ATPase; D012413:Rubidium