Improved cell depletion in a panning technique using covalent binding of immunoglobulins to surface modified polystyrene dishes.
A method for the chemical modification of plastic surfaces permits covalent binding of proteins and we have used this method in the development of an efficient panning technique. Thus, human peripheral T lymphocytes coated with mouse monoclonal antibodies directed against the CD4 marker may be selectively and reproducibly removed from a lymphocyte population by a short incubation in modified plastic dishes coated with rabbit anti-mouse IgG antibody. Due to the higher protein binding capacity of the dishes the use of the IgG fraction of the coating antibody was sufficient for optimal and reproducible results. In contrast, control dishes with passively adsorbed antibody required an affinity-purified fraction and even then were less efficient.
Larsson
P H
PH
Department of Clinical Immunology, Karolinska Hospital, Stockholm, Sweden.
Hed
J
J
Johansson
S G
SG
Persson
U
U
Wahlström
M
M
D015496:CD4-Positive T-Lymphocytes / Q000166:cytology; D002469:Cell Separation / Q000379:methods*; D055598:Chemical Phenomena; D002621:Chemistry; D003437:Crotonates; D006801:Humans; D011137:Polystyrenes; D011485:Protein Binding; D013601:T-Lymphocytes / Q000145:classification / Q000166:cytology*
D003437:Crotonates; D011137:Polystyrenes