[The effect of the synthetic analog of prostaglandin E2 sulprostone on the adenylate cyclase system in decidual tissue of women].
Activities of adenylate cyclase and phosphodiesterase were studied in decidual tissue of women, pregnancy of which was interrupted within the first term by means of synthetic analogue of prostaglandin E2 sulprostone; decidual tissue obtained after surgical abortion served as a control. Content of cAMP in decidual tissue was decreased 4-fold after administration of sulprostone. At the same time, activity od adenylate cyclase was decreased 2-3-fold, while activity of phosphodiesterase was unaltered. These data suggest that the decrease in cAMP concentration occurred as a result of inhibition of its synthesis. After administration of sulprostone the adenylate cyclase activity was decreased 3- and 4.5-fold in response to specific stimulators NaF and forskolin, respectively, thus indicating that content and activity of G protein, and apparently of catalytic subunits, were decreased. Long-term effect of prostaglandins on decidual tissue appears to cause a heterologous desensitization of adenylate cyclase and this phenomenon is responsible for pharmacologic action of prostaglandins.
Goncharova
V N
VN
Morozova
M S
MS
Razina
L G
LG
Astakhova
T M
TM
Manuilova
I A
IA
D000019:Abortifacient Agents / Q000494:pharmacology*; D000020:Abortifacient Agents, Nonsteroidal / Q000494:pharmacology*; D000067956:Adenylyl Cyclase Inhibitors; D000242:Cyclic AMP / Q000378:metabolism; D003656:Decidua / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D015232:Dinoprostone / Q000031:analogs & derivatives* / Q000494:pharmacology; D005260:Female; D019204:GTP-Binding Proteins / Q000378:metabolism; D006801:Humans; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D011247:Pregnancy
D000019:Abortifacient Agents; D000020:Abortifacient Agents, Nonsteroidal; D000067956:Adenylyl Cyclase Inhibitors; C016767:sulprostone; D000242:Cyclic AMP; D010727:Phosphoric Diester Hydrolases; D019204:GTP-Binding Proteins; D015232:Dinoprostone
Vliianie sinteticheskogo analoga prostaglandina E2 sul'prostona na adenilattsiklaznuiu sistemu v detsidual'noĭ tkani zhenshchin.