A solid-phase extraction procedure for DNA purification.
The preparation and use of particulate materials for the removal of proteins from nucleic acid samples by solid-phase extraction procedures are described. The solid-phase extraction procedure is analogous to the classical phenol extraction for DNA purification, with the exception that the phenol is replaced with insoluble particulate materials that are chemically similar to phenol and thus function in an analogous manner. These particulate materials have a very high affinity for proteins and a very low affinity for nucleic acids. With these materials, it is possible to remove large quantities of proteins (i.e., tens of milligrams) from minute quantities (submicrogram) of nucleic acid and quantitatively recover the latter in a biologically active state. Compared to other procedures that are currently used to purify nucleic acids, the protocols using these materials offer the advantages of speed, quantitative DNA recovery, safety, and convenience.
McCormick
R M
RM
E.I. du Pont de Nemours and Company, Inc., Experimental Station, Wilmington, Delaware 19880-0228.
D000469:Alkaline Phosphatase / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA / Q000097:blood / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D003851:Deoxyribonucleases / Q000032:analysis; D004926:Escherichia coli; D005069:Evaluation Studies as Topic; D006801:Humans; D008722:Methods; D019800:Phenol; D010636:Phenols; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D012822:Silicon Dioxide
D010636:Phenols; D010761:Phosphorus Radioisotopes; D019800:Phenol; D012822:Silicon Dioxide; D004247:DNA; D003851:Deoxyribonucleases; D004262:DNA Restriction Enzymes; D000469:Alkaline Phosphatase