Interactions of thyroid hormones with L-(3H) glutamate binding sites, with special reference to N-methyl-D-aspartate receptors.
An L-(3H)glutamate binding assay was developed in which 82% of the specific binding is to a site that corresponds to the N-methyl-D-aspartate receptor. The effects of various anticonvulsants and thyroid hormones were investigated with this assay. Anticonvulsants did not affect L-(3H)glutamate binding, while L- and D-isomers of triiodothyronine and thyroxine inhibited L-(3H)glutamate binding significantly. Scatchard analysis showed that thyroid hormones interacted with L-(3H)glutamate binding competitively. The interactions of thyroid hormones with NMDA-sensitive L-(3H)glutamate binding may be one of the mechanisms by which these hormones affect the central nervous system.
Oguro
K
K
Department of Pediatrics, Faculty of Medicine, Kyoto University, Japan.
Ito
M
M
Tsuda
H
H
Mutoh
K
K
Shiraishi
H
H
Shirasaka
Y
Y
Mikawa
H
H
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology; D001667:Binding, Competitive / Q000187:drug effects; D004594:Electrophysiology; D005971:Glutamates / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D017470:Receptors, Glutamate; D016194:Receptors, N-Methyl-D-Aspartate; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D013570:Synaptic Membranes / Q000187:drug effects / Q000378:metabolism; D013963:Thyroid Hormones / Q000494:pharmacology*
D000596:Amino Acids; D000927:Anticonvulsants; D005971:Glutamates; D017470:Receptors, Glutamate; D016194:Receptors, N-Methyl-D-Aspartate; D017981:Receptors, Neurotransmitter; D013963:Thyroid Hormones