title: Methionine aminopeptidase gene of Escherichia coli is essential for cell growth.
abstract: We localized the methionine aminopeptidase (map) gene on the Escherichia coli chromosome next to the rpsB gene at min 4. Genetically modified strains with the chromosomal map gene under lac promoter control grew only in the presence of the lac operon inducer isopropyl-beta-thiogalactoside. Thus, methionine aminopeptidase is essential for cell growth.
authors.affiliation: Department of Microbial Genetics, Cetus Corporation, Emeryville, California 94608.
authors.forename: S Y
authors.initials: SY
authors.lastname: Chang
authors.forename: E C
authors.initials: EC
authors.lastname: McGary
authors.forename: S
authors.initials: S
authors.lastname: Chang
mesh_terms: D000626:Aminopeptidases / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D002874:Chromosome Mapping; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D005798:Genes, Bacterial; D063208:Methionyl Aminopeptidases
chemical_list: D001426:Bacterial Proteins; D000626:Aminopeptidases; D063208:Methionyl Aminopeptidases