Comparison of LTB4- and C5a-stimulated chemotaxis of isolated human neutrophils: difference revealed by cell migration in thick filters using the multiwell cap procedure.
Under comparable conditions (90 min incubation in 2% albumin buffer) using 3 micron pore cellulose nitrate filters and the multiwell cap procedure (Evans et al., Bioscience Reports 6:1041, 1986), C5a was more potent than LTB4 as a chemoattractant (EC'50 s = 0.5 and 4 nM) and caused 5 times as many cells to completely traverse the filter. During the 90 min incubation, no cells traversed the filter in the absence of chemoattractant. The cap assay was modified to study migration of cells within the filter. During the 30 min incubation, EC50 values for C5a and LTB4 were comparable (1.2 and 1.6 nM) in causing cells to enter the filters but C5a was superior in causing the cells to move as measured by the mean distance of migration (EC'50 s = 0.25 and 1.8 nM). Our studies support the view that LTB4 acts primarily to cause cell adhesion (and penetration) of the endothelium and that C5a plays a major role in cell migration (McMillan and Foster, Agents and Actions 24: 114, 1988).
Psychoyos
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Research Department, Pharmaceuticals Division Ciba-Geigy Corporation Summit, NJ 07901.
Uziel-Fusi
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D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D015936:Complement C5a / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007975:Leukotriene B4 / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects*; D011994:Recombinant Proteins / Q000494:pharmacology
D011994:Recombinant Proteins; D007975:Leukotriene B4; D015936:Complement C5a