Site specific antibodies directed to the ATP binding region of some kinases.
Polyclonal antibodies raised in rabbits to ATP-requiring enzymes such as 3-phosphoglycerate kinase show cross-reactivity against other unrelated kinases. Our results show that rabbit polyclonal antiserum possesses antibodies that recognize an antigenic site at the ATP binding region of kinases. A classical immunotitration curve was obtained when hexokinase was titrated against anti-myokinase IgG. The immunoinhibitions was reversed in the presence of small concentration of ATP. This cross-reactivity between site specific antibody and unrelated kinase demonstrates the existence of an antigenic site around the ATP binding region. Our proposal of the existence of a common antigenic determinant in the ATP binding region is in agreement with the finding of a common structural domain that binds ATP.
Katiyar
S S
SS
Department of Chemistry, Indian Institute of Technology, Kanpur.
Srivastava
A
A
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000263:Adenylate Kinase / Q000276:immunology / Q000378:metabolism; D000818:Animals; D000906:Antibodies; D001665:Binding Sites; D003429:Cross Reactions; D006593:Hexokinase / Q000276:immunology / Q000378:metabolism; D007700:Kinetics; D010735:Phosphoglycerate Kinase / Q000276:immunology / Q000378:metabolism; D010770:Phosphotransferases / Q000276:immunology / Q000378:metabolism*; D013552:Swine
D000906:Antibodies; D000255:Adenosine Triphosphate; D010770:Phosphotransferases; D006593:Hexokinase; D010735:Phosphoglycerate Kinase; D000263:Adenylate Kinase