A 60 kd cdc2-associated polypeptide complexes with the E1A proteins in adenovirus-infected cells.
p60 is a cellular protein that binds to the adenovirus E1A protein complex in virally infected or transformed human cells. In both infected and uninfected cells, p60 was found in a complex with the cdc2 protein kinase. Immune complexes containing p60 and cdc2 display a cell cycle-dependent histone H1 kinase activity that is most active in interphase. The previously described cdc2-p62/cyclin complex also acts as a histone H1 kinase but is maximally active in mitotic metaphase. The shift in the timing of activation of different cdc2-containing complexes suggests that each might play a distinct role in regulation of the cell cycle.
Giordano
A
A
Cold Spring Harbor Laboratory, New York 11724.
Whyte
P
P
Harlow
E
E
Franza
B R
BR
Beach
D
D
Draetta
G
G
D017456:Adenovirus Early Proteins; D016203:CDC2 Protein Kinase; D002453:Cell Cycle; D004268:DNA-Binding Proteins / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D006367:HeLa Cells; D006801:Humans; D046911:Macromolecular Substances; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000378:metabolism*; D009856:Oncogene Proteins, Viral / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D011233:Precipitin Tests; D018809:Proliferating Cell Nuclear Antigen; D006656:Protamine Kinase / Q000378:metabolism; D011485:Protein Binding
D017456:Adenovirus Early Proteins; D004268:DNA-Binding Proteins; D046911:Macromolecular Substances; D009687:Nuclear Proteins; D009856:Oncogene Proteins, Viral; D010750:Phosphoproteins; D018809:Proliferating Cell Nuclear Antigen; D006656:Protamine Kinase; D016203:CDC2 Protein Kinase