title: Differential IL-2 receptor expression in renal allograft recipients treated with an anti-IL-2-receptor antibody.
abstract: Patients were entered into a randomized trial of prophylaxis for renal allograft rejection by the administration of an anti-human IL-2 receptor antibody, anti-Tac, during the first ten days posttransplant. Interleukin-2 receptor (IL-2 R) expression was measured using two anti-IL-2 R monoclonal antibodies (moAbs), anti-Tac and 1HT4-4H3. These two antibodies recognize closely spaced epitopes on the 55 kD chain of the IL-2 R. IL-2 R expression was examined on peripheral blood small lymphocytes in three groups of patients who received: (A) cyclosporine CsA and prednisone for baseline immunosuppression (n = 9); (B) anti-Tac with CsA and prednisone as baseline immunosuppression (n = 12); and (C) anti-Tac with azathioprine and prednisone as baseline immunosuppression (n = 5). We found that large numbers of T cells express IL-2 receptors despite the presence of anti-Tac (average of IL-2 R-positive cells at day of peak IL-2 R expression 56.0 +/- 20.8% in group A, 65.2 +/- 26.6% in group B, 21.0 +/- 7.4% in group C). IL-2 R expression did not correlate with clinical activity, and the presence or accessibility of epitopes on the same 55 kD chain varied dramatically from patient to patient.
authors.affiliation: Department of Medicine, Brigham and Women's Hospital, Beth Israel Hospital, Boston, Massachusetts 02115.
authors.forename: E L
authors.initials: EL
authors.lastname: Ramos
authors.forename: E L
authors.initials: EL
authors.lastname: Milford
authors.forename: R L
authors.initials: RL
authors.lastname: Kirkman
authors.forename: N L
authors.initials: NL
authors.lastname: Tilney
authors.forename: T B
authors.initials: TB
authors.lastname: Strom
authors.forename: M E
authors.initials: ME
authors.lastname: Shapiro
authors.forename: T A
authors.initials: TA
authors.lastname: Waldmann
authors.forename: I G
authors.initials: IG
authors.lastname: Wood
authors.forename: M R
authors.initials: MR
authors.lastname: Rollins
authors.forename: C B
authors.initials: CB
authors.lastname: Carpenter
mesh_terms: D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D001379:Azathioprine / Q000627:therapeutic use; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D016827:CD8 Antigens; D003524:Cyclosporins / Q000627:therapeutic use; D005434:Flow Cytometry; D006084:Graft Rejection; D006801:Humans; D007165:Immunosuppression Therapy / Q000379:methods; D016030:Kidney Transplantation; D011239:Prednisolone / Q000627:therapeutic use; D015375:Receptors, Interleukin-2 / Q000032:analysis*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D016827:CD8 Antigens; D003524:Cyclosporins; D015375:Receptors, Interleukin-2; D011239:Prednisolone; D001379:Azathioprine