Determination of the A-T content of DNA by second-derivative ultraviolet spectroscopy.
A method for the determination of the A-T content of DNA based on second-derivative ultraviolet spectra is presented. It allows measurement in a wide range of pH values, ionic strengths, and buffer media. It is nondestructive for the sample and requires not more than 10 micrograms of DNA.
Marquet
R
R
Laboratoire de Chimie Macromoléculaire et Chimie Physique, Université de Liège, Belgium.
Houssier
C
C
D000225:Adenine / Q000032:analysis*; D001482:Base Composition; D002696:Chlamydomonas / Q000032:analysis; D003016:Clostridium perfringens / Q000032:analysis; D004247:DNA / Q000032:analysis*; D004926:Escherichia coli / Q000032:analysis; D008837:Micrococcus / Q000032:analysis; D011067:Poly dA-dT / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet; D013941:Thymine / Q000032:analysis*
D011067:Poly dA-dT; D004247:DNA; D000225:Adenine; D013941:Thymine