title: Measurement of angiotensin II concentration in rat plasma: pathophysiological applications.
abstract: A very reliable isocratic reverse phase high performance liquid chromatography (HPLC) system has been developed to separate angiotensins, which combined with a very sensitive radioimmunoassay, provided precise measurements of the endogenous angiotensin II (AII) concentration in the rat plasma in different experimental circumstances. The overall recoveries of AII were 95.2 +/- 15.8% (means +/- SD) when 10 pg of this peptide was added to 1 ml of human plasma. The coefficient of variation for within-assay precision was 10% (n = 6). The plasma AII, measured by this method, of normal male pentobarbital-anesthetized rats was 53.0-141.6 pg/ml (mean: 103.9 +/- 29.7 pg/ml). The plasma AII of rats fed a sodium deficient diet was 300.0 +/- 100.6 pg/ml, while that of rats given oral Enalapril, an angiotensin converting enzyme (ACE) inhibitor, for 1 week was 35.7 +/- 28.0 pg/ml. The plasma AII of bilaterally nephrectomized rats was 2.7 +/- 2.9 pg/ml 24 hours after nephrectomy and below the detection limit 48 hr after nephrectomy. This method, therefore, can be used to study AII in different pathophysiological states or after treatment with renin-angiotensin system inhibitors.
authors.affiliation: INSERM Unit 36-17, Paris, France.
authors.forename: H
authors.initials: H
authors.lastname: Huang
authors.forename: T
authors.initials: T
authors.lastname: Baussant
authors.forename: R
authors.initials: R
authors.lastname: Reade
authors.forename: J B
authors.initials: JB
authors.lastname: Michel
authors.forename: P
authors.initials: P
authors.lastname: Corvol
mesh_terms: D000704:Analysis of Variance; D000758:Anesthesia; D000804:Angiotensin II / Q000097:blood*; D000805:Angiotensin III / Q000097:blood; D000809:Angiotensins / Q000097:blood; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004656:Enalapril / Q000494:pharmacology; D008297:Male; D009994:Osmolar Concentration; D010424:Pentobarbital; D010446:Peptide Fragments / Q000097:blood; D011863:Radioimmunoassay; D051381:Rats / Q000097:blood*; D011919:Rats, Inbred Strains; D012083:Renin / Q000097:blood
chemical_list: D000809:Angiotensins; D010446:Peptide Fragments; D000804:Angiotensin II; D000805:Angiotensin III; C022782:angiotensin pentapeptide; D004656:Enalapril; D012083:Renin; D010424:Pentobarbital