title: A kinetic analysis of lymphocyte 5'-nucleotidase in B-cell clones from control subjects and patients with primary hypogammaglobulinaemia.
abstract: The affinity of lymphocyte 5'-nucleotidase (5NT) for its substrate adenosine monophosphate (AMP) was studied in Epstein-Barr virus immortalized B-lymphocyte clones from healthy controls and patients with primary hypogammaglobulinaemia. Km values for AMP for 5NT in most of the patient clones were found to be significantly increased compared to B-cell clones from normal subjects, whereas Vmax values were within the normal range.
authors.affiliation: Division of Clinical Cell Biology, MRC Clinical Research Centre, Harrow, Middlesex, UK.
authors.forename: S E
authors.initials: SE
authors.lastname: Barwick
authors.forename: M
authors.initials: M
authors.lastname: Malkovsky
authors.forename: A D
authors.initials: AD
authors.lastname: Webster
authors.forename: T J
authors.initials: TJ
authors.lastname: Peters
mesh_terms: D015720:5'-Nucleotidase / Q000032:analysis*; D000249:Adenosine Monophosphate / Q000378:metabolism; D000361:Agammaglobulinemia / Q000201:enzymology* / Q000473:pathology; D001402:B-Lymphocytes / Q000201:enzymology* / Q000473:pathology; D002461:Cell Line, Transformed; D004854:Herpesvirus 4, Human; D006801:Humans; D007700:Kinetics
chemical_list: D000249:Adenosine Monophosphate; D015720:5'-Nucleotidase