High NaCl diets increase alpha 2-adrenoceptors in renal cortex and medulla of NaCl-sensitive spontaneously hypertensive rats.
Diets high in NaCl simultaneously elevate renal alpha 2-adrenoceptor binding and exacerbate hypertension in young NaCl-sensitive spontaneously hypertensive rats (SHR-S). The present study tests the hypothesis that in SHR-S on a high NaCl diet, an upregulation of renal alpha 2-adrenoceptors is present in densely innervated areas of the kidney, and this precedes the increase in blood pressure. Seven week old SHR-S fed on a high (8%) compared to basal (1%) NaCl diet for 2 weeks displayed significantly exacerbated hypertension and elevated renal alpha 2-adrenoceptor binding in both cortex and medulla. In contrast one week on the high NaCl diet did not alter renal alpha 2-adrenoceptor number or blood pressure in SHR-S. Autoradiographic experiments demonstrated that the NaCl-induced upregulation of alpha 2-adrenoceptors occurs in all areas of the renal cortex and medulla. None of these differences were observed in NaCl-resistant, Wistar-Kyoto rats (WKY). Further, the high NaCl diet did not alter renal alpha 1-adrenoceptor binding in SHR-S or WKY. Together with previous findings, these data suggest that the NaCl-induced upregulation of renal alpha 2-adrenoceptors is not specific to densely innervated regions of the kidney.
Sripairojthikoon
W
W
Department of Cell Biology and Anatomy, University of Alabama, Birmingham 35294.
Wyss
J M
JM
D000818:Animals; D001345:Autoradiography; D002395:Catecholamines / Q000378:metabolism; D004032:Diet; D006973:Hypertension / Q000378:metabolism*; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism*; D007679:Kidney Medulla / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D011942:Receptors, Adrenergic, alpha / Q000187:drug effects*; D012965:Sodium Chloride / Q000494:pharmacology*; D013997:Time Factors
D002395:Catecholamines; D011942:Receptors, Adrenergic, alpha; D012965:Sodium Chloride