Xylamine enhances pineal gland N-acetyltransferase activity in vitro.
1. The action of N-2'-chloroethyl-N-ethyl-2-methyl benzylamine (xylamine) on rat pineal gland sympathetic innervation was examined. 2. This alkylating agent caused a concentration-dependent increase in pineal gland N-acetyltransferase (NAT) activity in neurologically intact pineal glands that was suppressed in glands previously subjected to bilateral superior cervical ganglionectomy. 3. Xylamine-induced elevations in NAT activity were attenuated by beta-noradrenergic antagonist drugs but not by alpha-noradrenergic antagonist drugs. 4. Since pineal gland uptake of radiolabelled norepinephrine (NE) was impaired by xylamine, the drug may increase pineal gland NAT activity by inhibiting NE reuptake into the presynaptic nerve terminal, thereby increasing the amount of the neurotransmitter available to stimulate pinealocyte beta-noradrenoceptors.
Haak
K A
KA
Department of Psychology, University of California, Santa Barbara 93106.
Torres
G
G
Garvey
P L
PL
Bronstein
D M
DM
Cho
A K
AK
Lytle
L D
LD
D000123:Acetyltransferases / Q000037:antagonists & inhibitors*; D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D003714:Denervation; D004347:Drug Interactions; D066298:In Vitro Techniques; D008297:Male; D009588:Nitrogen Mustard Compounds / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D009638:Norepinephrine / Q000378:metabolism; D010870:Pineal Gland / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013566:Sympathomimetics / Q000037:antagonists & inhibitors / Q000494:pharmacology*
D000317:Adrenergic alpha-Antagonists; D000319:Adrenergic beta-Antagonists; D009588:Nitrogen Mustard Compounds; D013566:Sympathomimetics; C026131:xylamine; D000123:Acetyltransferases; D009638:Norepinephrine