Purification of the mitochondrial carnitine carrier by chromatography on hydroxyapatite and celite.
The carnitine carrier from rat liver mitochondria has been extracted with Triton X-100 ad partially purified by chromatography on hydroxyapatite and celite. During purification the activity of the carrier was monitored by functional reconstitution into liposomes. The purified fraction is 250-fold enriched with respect to the N-ethylmaleimide-sensitive carnitine/carnitine transport activity. The substrate specificity and the inhibitor sensitivity of carnitine transport in liposomes resemble closely those described for the transport of carnitine in mitochondria.
Indiveri
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Dipartimento Farmaco-iologico, Università di Bari, Italy.
Palmieri
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D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002331:Carnitine / Q000378:metabolism*; D002352:Carrier Proteins / Q000302:isolation & purification*; D002845:Chromatography / Q000379:methods; D007692:Diatomaceous Earth; D017886:Durapatite; D006882:Hydroxyapatites; D008930:Mitochondria, Liver / Q000032:analysis*; D008970:Molecular Weight; D051381:Rats; D013379:Substrate Specificity
D002352:Carrier Proteins; D006882:Hydroxyapatites; D007692:Diatomaceous Earth; D017886:Durapatite; D002331:Carnitine