Evaluation of the mutagenicity and the tumor-promoting activity of parasite extracts: Schistosoma japonicum and Clonorchis sinensis.
In relation to the observed association of carcinogenesis with parasitic infections, the mutagenicity of extracts of Schistosoma japonicum and Clonorchis sinensis was examined. In the bacterial mutagenicity tests using the Ames Salmonella typhimurium strains TA98, TA100, TA97 and TA102, and Escherichia coli WP2 and WP2 uvrA pKM101 Schistosoma soluble egg antigen and a homogenate of adult Schistosoma worms showed no positive responses either in the presence or in the absence of S9 mix. Likewise, adult worm extracts of Clonorchis showed no mutagenicity. The Schistosoma soluble egg antigen showed a weak but significant activity for the induction of Epstein-Barr virus expression in viral genome-carrying human lymphoblastoid cells in culture. This phenomenon suggests that the soluble egg antigen possesses tumor-promoting activity.
Ishii
A
A
Department of Parasitology, Okayama University Medical School, Japan.
Matsuoka
H
H
Aji
T
T
Hayatsu
H
H
Wataya
Y
Y
Arimoto
S
S
Tokuda
H
H
D000818:Animals; D002273:Carcinogens; D003004:Clonorchis sinensis / Q000378:metabolism*; D015870:Gene Expression; D004854:Herpesvirus 4, Human / Q000235:genetics; D009152:Mutagenicity Tests; D009153:Mutagens; D012486:Salmonella typhimurium / Q000235:genetics*; D012549:Schistosoma japonicum / Q000378:metabolism*
D002273:Carcinogens; D009153:Mutagens