title: Production of transgenic merino sheep by microinjection of ovine metallothionein-ovine growth hormone fusion genes.
abstract: Seven transgenic Merino sheep have been produced by the technique of pronuclear microinjection. Two different Sheep Metallothionein-1a-Sheep Growth Hormone fusion genes were used. Four of the transgenic sheep, all of which contained the gene MTsGH5, did not express the transgene. The remaining three sheep carrying the second fusion gene, MTsGH9, expressed the gene at high levels in a variety of tissues and had elevated blood levels of sheep growth hormone.
authors.affiliation: Department of Animal Science, University of California, Davis 95616.
authors.forename: J D
authors.initials: JD
authors.lastname: Murray
authors.forename: C D
authors.initials: CD
authors.lastname: Nancarrow
authors.forename: J T
authors.initials: JT
authors.lastname: Marshall
authors.forename: I G
authors.initials: IG
authors.lastname: Hazelton
authors.forename: K A
authors.initials: KA
authors.lastname: Ward
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D004624:Embryo Transfer; D004971:Estrus; D005187:Fallopian Tubes / Q000502:physiology; D005260:Female; D005298:Fertility; D013006:Growth Hormone / Q000235:genetics*; D008668:Metallothionein / Q000235:genetics*; D008845:Microinjections; D011247:Pregnancy; D012756:Sheep / Q000235:genetics*; D013539:Simian virus 40 / Q000235:genetics
chemical_list: D004279:DNA, Viral; D013006:Growth Hormone; D004247:DNA; D008668:Metallothionein