Calcium channels in undifferentiated PC12 rat pheochromocytoma cells.
Undifferentiated rat pheochromocytoma PC12 cells were voltage clamped using the whole cell technique. After blockade of outward currents, calcium currents were elicited from -40 and -100 mV. A subpopulation of cells displayed only one current component activated at -10 mV and slowly decaying. In other cells this current coexisted with a component activated around -40 mV and decaying with a faster time constant. We conclude that undifferentiated PC12 cells can express two types of calcium channels, L (long-lasting) and N (neuronal)-type channels.
Janigro
D
D
Department of Pharmacology, S. Raffaele Scientific Institute, University of Milan, Italy.
Maccaferri
G
G
Meldolesi
J
J
D000310:Adrenal Gland Neoplasms / Q000503:physiopathology*; D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology; D015220:Calcium Channels / Q000187:drug effects / Q000502:physiology*; D002460:Cell Line; D004553:Electric Conductivity; D008564:Membrane Potentials; D008978:Mollusk Venoms / Q000494:pharmacology; D009568:Nitrendipine / Q000494:pharmacology; D010673:Pheochromocytoma / Q000503:physiopathology*; D051381:Rats; D014407:Tumor Cells, Cultured / Q000502:physiology*; D020960:omega-Conotoxins
D002121:Calcium Channel Blockers; D015220:Calcium Channels; D008978:Mollusk Venoms; D020960:omega-Conotoxins; C053423:Conus magus toxin; D009568:Nitrendipine