A strategy simplifying site-directed mutagenesis in the CD4-binding region of HIV gp 120.
By site-directed mutagenesis, two unique restriction sites, surrounding the CD4-binding DNA region of HIV gp 120, were created. The mutations do not cause amino acid substitutions but generate a DNA fragment, which is stable when cloned into M 13 for further mutagenesis and is also easy to reclone.
Hemming
A
A
Department of Clinical Virology, University of Göteborg, Sweden.
Lundberg
L
L
Olofsson
S
S
D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000235:genetics; D001665:Binding Sites; D003001:Cloning, Molecular; D003062:Codon; D005822:Genetic Vectors; D015699:HIV Envelope Protein gp120 / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D009841:Oligonucleotides / Q000138:chemical synthesis; D015728:Receptors, HIV / Q000378:metabolism*
D003062:Codon; D015699:HIV Envelope Protein gp120; D009841:Oligonucleotides; D015728:Receptors, HIV