title: Involvement of c-myc oncogene in lymphoma cell lines with no detectable chromosome rearrangement of band 8q24.
abstract: Two lymphoma cell lines of B-cell type were established from Japanese patients with diffuse small noncleaved cell lymphoma. Cytogenetic analysis revealed a 14q+ marker chromosome in both cell lines, and a t(8;14)(q24.1;q32.3) seemed most likely to have occurred. The chromosome 8 pair, however, had no abnormalities. Molecular analysis demonstrated c-myc amplification lacking gross rearrangement in one cell line and genetic rearrangement of c-myc at the first intron as well as aberrant sizes of c-myc mRNA in the other cell line. In the latter case, it is possible that a t(8;14)(q24.1;q32.3) was buried in an unrecognized complex translocation. A combination of cytogenetic and molecular studies to determine the precise nature of the 14q32 translocation is discussed.
authors.affiliation: Department of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ohno
authors.forename: S
authors.initials: S
authors.lastname: Fukuhara
authors.forename: S
authors.initials: S
authors.lastname: Doi
authors.forename: R
authors.initials: R
authors.lastname: Amakawa
authors.forename: M
authors.initials: M
authors.lastname: Horii
authors.forename: Y
authors.initials: Y
authors.lastname: Akiyama
authors.forename: W
authors.initials: W
authors.lastname: Fukuda
authors.forename: T
authors.initials: T
authors.lastname: Honjo
authors.forename: T
authors.initials: T
authors.lastname: Sugiyama
authors.forename: H
authors.initials: H
authors.lastname: Uchino
mesh_terms: D000368:Aged; D015152:Blotting, Northern; D015139:Blotting, Southern; D002675:Child, Preschool; D002871:Chromosome Banding; D002883:Chromosomes, Human, Pair 14; D002898:Chromosomes, Human, Pair 8; D004262:DNA Restriction Enzymes; D005819:Genetic Markers; D006801:Humans; D007621:Karyotyping; D008223:Lymphoma / Q000235:genetics*; D008297:Male; D011519:Proto-Oncogenes; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
chemical_list: D005819:Genetic Markers; D004262:DNA Restriction Enzymes