title: Purification of the mitochondrial carnitine carrier by chromatography on hydroxyapatite and celite.
abstract: The carnitine carrier from rat liver mitochondria has been extracted with Triton X-100 ad partially purified by chromatography on hydroxyapatite and celite. During purification the activity of the carrier was monitored by functional reconstitution into liposomes. The purified fraction is 250-fold enriched with respect to the N-ethylmaleimide-sensitive carnitine/carnitine transport activity. The substrate specificity and the inhibitor sensitivity of carnitine transport in liposomes resemble closely those described for the transport of carnitine in mitochondria.
authors.affiliation: Dipartimento Farmaco-iologico, Università di Bari, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Indiveri
authors.forename: F
authors.initials: F
authors.lastname: Palmieri
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002331:Carnitine / Q000378:metabolism*; D002352:Carrier Proteins / Q000302:isolation & purification*; D002845:Chromatography / Q000379:methods; D007692:Diatomaceous Earth; D017886:Durapatite; D006882:Hydroxyapatites; D008930:Mitochondria, Liver / Q000032:analysis*; D008970:Molecular Weight; D051381:Rats; D013379:Substrate Specificity
chemical_list: D002352:Carrier Proteins; D006882:Hydroxyapatites; D007692:Diatomaceous Earth; D017886:Durapatite; D002331:Carnitine