title: Phenotypic characterization of bovine lymphoblastoid cell lines.
abstract: Cytochemical and immunological markers were used to phenotype the bovine lymphoblastoid cell lines BL-3*, EBL-1, and EBL-2. Southern blot experiments were also performed to test these lines for the presence of proviral bovine leukemia virus (BLV). The BL-3* cell line, originally derived from a case of sporadic bovine leukosis (non BLV-associated) but later infected with BLV in vitro, was found to contain BLV provirus and expressed the BLV-encoded envelope glycoprotein BLV-gp51. BL-3* cells express surface IgM and cytoplasmic IgM as well as class II antigens, and greater than 95% were negative for the T-cell markers B26A, sheep erythrocyte (E) receptors and alpha-naphthyl butyrate esterase (alpha-NB). BL-3* thus appears to be B-cell derived. EBL-1 and EBL-2 were derived from cows with enzootic bovine lymphosarcoma; however, these cell lines were found not to be infected with BLV. Phenotypically, EBL-1 and EBL-2 are mature T-cells, as they were positive for the B26A epitope, alpha-NB, and E receptors. These cell lines also express class II major histocompatibility antigens, indicating an activated state. The T-cell phenotype of EBL-1 and EBL-2 raises interesting questions concerning the possible role of other retroviruses and non BLV-infected transformed T-cells in the development of EBL tumors.
authors.affiliation: Department of Animal Sciences, University of Illinois, Urbana 61801.
authors.forename: M J
authors.initials: MJ
authors.lastname: Romano
authors.forename: J A
authors.initials: JA
authors.lastname: Stewart
authors.forename: H A
authors.initials: HA
authors.lastname: Lewin
mesh_terms: D000818:Animals; D015415:Biomarkers; D002417:Cattle; D002454:Cell Differentiation; D002460:Cell Line; D006651:Histocytochemistry; D001909:Leukemia Virus, Bovine / Q000302:isolation & purification; D008214:Lymphocytes / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D010641:Phenotype
chemical_list: D015415:Biomarkers