title: Studies on CD11a and CD18 molecules with two new monoclonal antibodies: differential myelomonocytic antigen expression of PMA treated HL60 and U937 cell lines.
abstract: Biochemical and functional aspects as well as features of cellular distribution of the differentiation groups CD11a and CD18 were studied in the course of a detailed characterization of two new monoclonal antibodies which recognize the alpha (GRS3) and beta (GRF1) chains of the LFA-1 antigen. Both MAbs inhibited homotypic aggregation of an EBV cell line. In contrast, only GRF1 (anti-beta chain) was able to inhibit granulocyte aggregation as well. Different myeloid-monocyte antigen modulation was noted in PMA induced macrophage differentiation of the U937 and HL60 cell lines. PMA treated HL60 cells showed increased expression of alpha M (CD11b) and alpha X (CD11c) antigens but no change in HLA-DR or CD14 antigen expression. No variation in the expression of LFA complex antigen (CD11a, b and c, or CD18) was observed on U937 cells, which on the other hand presented de novo expression of HLA-DR and CD14 antigens.
authors.affiliation: Hospital Virgen de las Nieves, Laboratorio de Análisis Clínicos e Immunología, Granada, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Pedrinaci
authors.forename: C
authors.initials: C
authors.lastname: Huelin
authors.forename: M
authors.initials: M
authors.lastname: Patarroyo
authors.forename: F
authors.initials: F
authors.lastname: Ruiz-Cabello
authors.forename: F
authors.initials: F
authors.lastname: Garrido
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D000943:Antigens, Differentiation / Q000276:immunology*; D015214:Antigens, Differentiation, Myelomonocytic / Q000276:immunology*; D018821:CD18 Antigens; D002448:Cell Adhesion; D005434:Flow Cytometry; D006801:Humans; D016169:Lymphocyte Function-Associated Antigen-1; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins / Q000276:immunology*; D008970:Molecular Weight; D011233:Precipitin Tests; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D015214:Antigens, Differentiation, Myelomonocytic; D018821:CD18 Antigens; D016169:Lymphocyte Function-Associated Antigen-1; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins; D013755:Tetradecanoylphorbol Acetate