title: Calcium dependent cysteine proteinase is a precursor of a chemotactic factor for neutrophils.
abstract: A new chemotactic factor for neutrophils is generated from calcium dependent cysteine proteinase (calpain) I by autodigestion. An active peptide was isolated from the autodigest and its structure was determined to be an acetylated nonapeptide with the sequence: N-acetyl Ser-Glu-Glu-Ile-Ile-Thr-Pro-Val-Tyr. Compared with the entire sequence of human calpain I, the peptide was identical with the N-terminal amino acid sequence of the large subunit deduced from the cDNA sequence, except that the peptide was devoid of a methionine residue and acetylated at the N-terminus. The acetyl nonapeptide was synthesized and its chemotactic activity was reconfirmed. The biological significance and possible role of this calpain derived chemotactic factor in inflammation are discussed.
authors.affiliation: Department of Biochemistry, Nagoya City University Medical School, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kunimatsu
authors.forename: S
authors.initials: S
authors.lastname: Higashiyama
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: I
authors.initials: I
authors.lastname: Ohkubo
authors.forename: M
authors.initials: M
authors.lastname: Sasaki
mesh_terms: D000595:Amino Acid Sequence; D002154:Calpain / Q000097:blood* / Q000235:genetics; D002630:Chemotactic Factors / Q000096:biosynthesis / Q000097:blood* / Q000302:isolation & purification; D002634:Chemotaxis, Leukocyte; D004912:Erythrocytes / Q000201:enzymology; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009504:Neutrophils / Q000502:physiology*; D010196:Pancreatic Elastase / Q000097:blood
chemical_list: D002630:Chemotactic Factors; D010196:Pancreatic Elastase; D002154:Calpain