title: Detection of P-glycoprotein in human leukemias using monoclonal antibodies.
abstract: Overexpression of a Mr 170,000 membrane glycoprotein (P-glycoprotein) is consistently associated with multidrug resistance in cell lines. Two monoclonal antibodies (Mab) against P-glycoprotein (265/F4 and C 219) were used to examine tumour samples from patients with leukemias for evidence of P-glycoprotein overexpression. High levels of P-glycoprotein (greater than 5% positive cells) were detected with both antibodies in samples from 3 out of 18 patients suggesting that a multidrug resistant phenotype may also occur in human leukemias.
authors.affiliation: Department of Experimental Pathology, German Cancer Research Center, Heidelberg.
authors.forename: J
authors.initials: J
authors.lastname: Mattern
authors.forename: T
authors.initials: T
authors.lastname: Efferth
authors.forename: M
authors.initials: M
authors.lastname: Bak
authors.forename: A D
authors.initials: AD
authors.lastname: Ho
authors.forename: M
authors.initials: M
authors.lastname: Volm
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000911:Antibodies, Monoclonal; D001666:Binding Sites, Antibody; D004351:Drug Resistance / Q000235:genetics; D006801:Humans; D007938:Leukemia / Q000097:blood* / Q000188:drug therapy / Q000235:genetics; D008562:Membrane Glycoproteins / Q000097:blood* / Q000276:immunology; D009363:Neoplasm Proteins / Q000097:blood* / Q000276:immunology; D010641:Phenotype
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000911:Antibodies, Monoclonal; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins