Isolation of the facA (acetyl-coenzyme A synthetase) and acuE (malate synthase) genes of Aspergillus nidulans.
Acetate inducible genes of Aspergillus nidulans were cloned via differential hybridization to cDNA probes. Using transformation of mutant strains the genes were identified as facA (acetyl-Coenzyme A synthetase) and acuE (malate synthase). The levels of RNA encoded by these genes were shown to be acetate inducible and subject to carbon catabolite repression. Induction is abolished in a facB mutant and carbon catabolite repression is relieved in a creA mutant.
Sandeman
R A
RA
Department of Genetics, University of Melbourne, Parkville, Victoria, Australia.
Hynes
M J
MJ
D000106:Acetate-CoA Ligase / Q000235:genetics*; D000085:Acetates / Q000494:pharmacology; D001233:Aspergillus nidulans / Q000201:enzymology / Q000235:genetics*; D015152:Blotting, Northern; D015139:Blotting, Southern; D003066:Coenzyme A Ligases / Q000235:genetics*; D004251:DNA Transposable Elements; D005786:Gene Expression Regulation; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D008292:Malate Synthase / Q000235:genetics*; D009154:Mutation; D007652:Oxo-Acid-Lyases / Q000235:genetics*; D010957:Plasmids; D012331:RNA, Fungal / Q000032:analysis; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014170:Transformation, Genetic; D014178:Translocation, Genetic
D000085:Acetates; D004251:DNA Transposable Elements; D012331:RNA, Fungal; D008292:Malate Synthase; D007652:Oxo-Acid-Lyases; D003066:Coenzyme A Ligases; D000106:Acetate-CoA Ligase