title: Use of synthetic oligodeoxyribonucleotides for type-specific identification of coxsackie B viruses.
abstract: Synthetic oligodeoxyribonucleotides were used for type-specific identification of members of the coxsackie B virus group by nucleic acid hybridization. Two pairs of oligonucleotide chains were constructed based on nucleotide sequences in the VP1 regions of coxsackieviruses B3 and B4. Each labelled probe had a length of 24 nucleotides. The results showed that the oligonucleotide hybridized in a type-specific manner when assayed with extracts from cells infected with all different coxsackie B viruses. A method based on similar principles may thus be used for enterovirus typing.
authors.affiliation: Department of Medical Genetics, Uppsala University, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Alksnis
authors.forename: M
authors.initials: M
authors.lastname: Lindberg
authors.forename: P
authors.initials: P
authors.lastname: Stålhandske
authors.forename: H
authors.initials: H
authors.lastname: Hultberg
authors.forename: U
authors.initials: U
authors.lastname: Pettersson
mesh_terms: D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D029822:Enterovirus B, Human / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides
chemical_list: D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides