Cytotoxic effect of phenazine methosulphate on the isolated frog heart.
1. Perfusion of isolated frog hearts with phenazine methosulphate (PMS) at 0.3-1.0 mM caused a fall in amplitude and frequency of beat, and finally a cessation of contractile activity, together with widespread ultrastructural damage. 2. Sarcolemma blebs were a characteristic feature of the damage. 3. No protection was provided by mannitol (10-100 mM), superoxide dismutase, catalase or a pHo of 6.6. 4. Potassium ferricyanide (1-6 mM), an artificial electron acceptor, also caused ultrastructural damage. 5. Comparisons are made with the oxygen paradox of mammalian heart, and the possible role of Ca2+ fluxes and oxygen radicals in muscle damage are discussed.
Duncan
C J
CJ
Department of Zoology, University of Liverpool, UK.
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D006321:Heart / Q000187:drug effects*; D066298:In Vitro Techniques; D008773:Methylphenazonium Methosulfate / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D009200:Myocardial Contraction / Q000187:drug effects; D009206:Myocardium / Q000166:cytology* / Q000648:ultrastructure; D009994:Osmolar Concentration; D010477:Perfusion; D010619:Phenazines / Q000494:pharmacology*; D011896:Rana temporaria; D012518:Sarcomeres / Q000187:drug effects / Q000502:physiology
D010619:Phenazines; D008773:Methylphenazonium Methosulfate