The biphasic change of cytosolic acetyl-CoA hydrolase in rat liver during 3'-methyl-4-dimethylaminoazobenzene hepatocarcinogenesis.
When Donryu male albino rats were given diet containing 0.06% 3'-methyl-4-dimethyl-aminoazobenzene (3'-Me-DAB) for 20 weeks, the activity of cytosolic acetyl-CoA hydrolase in their livers decreased to about one-third the initial level in week 2, returned to the control level in week 7, and then decreased again to about one-tenth of the control in week 20. These changes in enzyme activity were parallel with changes in the amount of enzyme protein determined by ELISA. In 3'-Me-DAB-resistant rats, however, the enzyme activity and enzyme protein remained within the normal range during administration of 3'-Me-DAB-containing diet for 20 weeks and no tumors were detectable macroscopically. Interestingly, the biphasic change in this enzyme activity was inversely associated with the well known change of gamma-glutamyltranspeptidase activity during azo-dye-induced hepatocarcinogenesis.
Ebisuno
S
S
Department of Oncology, Osaka University Medical School.
Isohashi
F
F
Nakanishi
Y
Y
Higashi
T
T
Sakamoto
Y
Y
D000104:Acetyl-CoA Hydrolase / Q000378:metabolism*; D000818:Animals; D003600:Cytosol / Q000201:enzymology; D004797:Enzyme-Linked Immunosorbent Assay; D005779:Immunodiffusion; D008099:Liver / Q000201:enzymology* / Q000648:ultrastructure; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000201:enzymology*; D008297:Male; D008749:Methyldimethylaminoazobenzene; D051381:Rats; D013869:Thiolester Hydrolases / Q000378:metabolism*
D008749:Methyldimethylaminoazobenzene; D013869:Thiolester Hydrolases; D000104:Acetyl-CoA Hydrolase