Sequence of rat skeletal muscle phosphoglycerate mutase cDNA.
A cDNA clone coding rat skeletal muscle phosphoglycerate mutase was isolated from a rat muscle lambda gt10 cDNA library and its sequence was determined. The deduced protein possesses 252 amino acids and is 94% homologous with respect to human muscle phosphoglycerate mutase. No amino acids changes occur at the active site and structural predictions suggest strong conformational homologies with other enzymes of the mutase family.
Castellà-Escolà
J
J
Unitat de Bioquímica, Facultat de Medicina, Universitat de Barcelona, Spain.
Montoliu
L
L
Pons
G
G
Puigdomènech
P
P
Cohen-Solal
M
M
Carreras
J
J
Rigau
J
J
Climent
F
F
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D003062:Codon; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D009132:Muscles / Q000201:enzymology*; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D010736:Phosphoglycerate Mutase / Q000235:genetics*; D010770:Phosphotransferases / Q000235:genetics*; D011487:Protein Conformation; D051381:Rats; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D003062:Codon; D015345:Oligonucleotide Probes; D004247:DNA; D010770:Phosphotransferases; D010736:Phosphoglycerate Mutase