Multicentric independent development of hepatocellular carcinoma revealed by analysis of hepatitis B virus integration pattern.
Two separate nodular lesions of hepatocellular carcinoma (HCC) were surgically removed from a patient with positive serum hepatitis B surface antigen. Southern blot analysis of DNA from the two separate HCC lesions showed different restriction patterns of integrated hepatitis B virus DNA, indicating their independent origins. The two lesions were composed mainly of poorly differentiated HCC; however, well-differentiated HCC was also observed in the periphery of the lesions. This histological finding is consistent with the multistep and independent development of HCC in each site of the liver.
Sakamoto
M
M
Department of Surgery, National Cancer Center Research Institute and Hospital, Tokyo, Japan.
Hirohashi
S
S
Tsuda
H
H
Shimosato
Y
Y
Makuuchi
M
M
Hosoda
Y
Y
D000368:Aged; D015139:Blotting, Southern; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis* / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D008113:Liver Neoplasms / Q000175:diagnosis* / Q000235:genetics; D008297:Male; D009693:Nucleic Acid Hybridization
D004279:DNA, Viral