[A receptor of dihydropyridine blockaders of Ca2+ influx in human embryonic fibroblasts].
The plasma membrane of human embryonic fibroblasts was shown to contain receptor binding sites for 1,4-dihydropyridine (DHP) Ca2+ entry blockers. In a subconfluent culture grown in serum medium the content of the DHP receptor amounted to 1.2 +/- 0.3 pmol per 10(6) cells. With progression to confluency this value decreased up to 0.28 +/- 0.08 pmol per 10(6) cells. The DHP binding capacity was shown to be affected by the presence of growth factors in culture medium. In a subconfluent culture of serum-deprived cells the content of DHP binding sites increased 1.9 fold, the steady-state level being achieved within 3 days in culture. The serum gradually reversed this process, and the DHP receptor density approached the initial level within 3 days.
Dudkin
S M
SM
Gnedoĭ
S N
SN
Cherniuk
N N
NN
Soldatov
N M
NM
D002121:Calcium Channel Blockers; D015220:Calcium Channels; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D004095:Dihydropyridines / Q000493:pharmacokinetics*; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D011978:Receptors, Nicotinic / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors; D014316:Tritium
D002121:Calcium Channel Blockers; D015220:Calcium Channels; D004095:Dihydropyridines; D006133:Growth Substances; D008565:Membrane Proteins; D011978:Receptors, Nicotinic; D014316:Tritium
Retseptor digidropiridinovykh blokatorov vkhoda Ca2+ v émbrional'nykh fibroblastakh cheloveka.