title: Differentiation of avian adenovirus type-II strains by restriction endonuclease fingerprinting.
abstract: Three serologically indistinguishable viruses from the avian adenovirus type-II splenomegaly virus of chickens, marble spleen disease virus of pheasants, and hemorrhagic enteritis virus of turkeys, were analyzed by restriction endonuclease fingerprinting. The DNA from these viruses were examined with 6 restriction endonucleases (Bgl II, EcoRI, HindIII, Hha I, Xho I, and BamHI). Markedly different DNA cleavage patterns were found in these virus isolates with all the 5 enzymes, except with BamHI, suggesting genetic differences between isolates of adenovirus type II. Restriction endonuclease analyses were found to provide a method for distinguishing genetically different, and yet serologically similar, strains of avian adenovirus type II.
authors.affiliation: Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Minnesota, St. Paul 55108.
authors.forename: C
authors.initials: C
authors.lastname: Zhang
authors.forename: K V
authors.initials: KV
authors.lastname: Nagaraja
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000257:Adenoviridae Infections / Q000382:microbiology / Q000662:veterinary*; D000818:Animals; D000259:Aviadenovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis*; D008854:Microscopy, Electron; D009710:Nucleotide Mapping; D011201:Poultry Diseases / Q000382:microbiology*; D013045:Species Specificity; D014422:Turkeys / Q000382:microbiology*
chemical_list: D004279:DNA, Viral; D004262:DNA Restriction Enzymes