Initial characterization of lens beta-adrenergic receptors.
Crude plasma membranes were prepared from chick lens epithelial and fiber cells and assayed for specific binding of the beta-adrenergic antagonist dihydroalprenolol. Both membranes specifically bound the ligand, with the epithelial membranes exhibiting a greater number of higher affinity sites. This is the first demonstration of lens beta-adrenergic receptors using this type of assay and the first direct demonstration of beta-adrenergic receptors in lens fiber cells.
Ireland
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Department of Anatomy/Cell Biology, Wayne State University, School of Medicine, Detroit, Michigan.
Jacks
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D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002462:Cell Membrane / Q000378:metabolism; D004082:Dihydroalprenolol / Q000037:antagonists & inhibitors / Q000378:metabolism; D007908:Lens, Crystalline / Q000378:metabolism* / Q000648:ultrastructure; D009994:Osmolar Concentration; D011433:Propranolol / Q000378:metabolism; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D013997:Time Factors
D011943:Receptors, Adrenergic, beta; D004082:Dihydroalprenolol; D011433:Propranolol