[The blocking action of platelet-activating factor (PAF) on the calcium currents of the atrial fibers in the frog and guinea pig].
The effects of platelet-activating factor (PAF) on the myocardial cell membrane Ca-current (ICa) and Ca-action potential (Ca-AP) were investigated. In double sucrose-gap voltage-clamped frog atrial trabeculae PAF (2 X 10(-7) M) reduced ICa-amplitude to 40-50%; at the same time the IK-amplitude was increased to the same value. These changes of ICa and IK amplitudes were protected by simultaneous action of PAF and PAF antagonist BN 52021 (4 X 10(-6) M). In the partially depolarized (K+0 = 15-20 mM) of the guinea pig myocardial auricles PAF decreased Ca-AP amplitude and Vmax of its upstroke and shortened the Ca-AP duration (intracellular microelectrodes) like the isometric tension responses. These effects were prevented by PAF antagonist U-66985. Histamine was also able to protect from the PAF-induced changes of Ca-AP and tension responses. Our data demonstrated both by direct and by indirect methods of ICa registration in myocardia membrane that PAF induces reversed blocking of ICa. Because the blocking effects of PAF on frog and guinea pig myocardium are identical, these results imply that the mechanisms of PAF action on cold- and warm-blooded animals are similar in principle. The coupling of ICa and IK changes confirm our earlier supposition that PAF-induced Ca-AP shorting can be explained by IK augmentation.
Vornovitskiĭ
E G
EG
Kobrinskiĭ
E M
EM
Gallasch
M
M
Ignat'eva
V B
VB
Kukushkin
N I
NI
D000818:Animals; D016275:Atrial Function; D002121:Calcium Channel Blockers; D015220:Calcium Channels / Q000187:drug effects* / Q000502:physiology; D004224:Diterpenes; D046934:Ginkgolides; D006168:Guinea Pigs; D006321:Heart / Q000187:drug effects* / Q000502:physiology; D006325:Heart Atria / Q000187:drug effects; D006632:Histamine / Q000494:pharmacology; D066298:In Vitro Techniques; D007783:Lactones / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009200:Myocardial Contraction / Q000187:drug effects / Q000502:physiology; D010972:Platelet Activating Factor / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D011895:Rana ridibunda
D002121:Calcium Channel Blockers; D015220:Calcium Channels; D004224:Diterpenes; D046934:Ginkgolides; D007783:Lactones; D010972:Platelet Activating Factor; D006632:Histamine; C046779:U 66985; C045856:ginkgolide B
Blokiruiushchee deĭstvie faktora aktivatsii trombotsitov (PAF) na kal'tsievye toki volokon predserdiia liagushki i morskoĭ svinki.