Nucleotide sequence of a neurovirulent variant of the type 2 oral poliovirus vaccine.
Infectious cDNAs of the Sabin type 2 poliovirus vaccine virus and a vaccine-derived neurovirulent type 2 strain (P2/117) have been cloned in Escherichia coli. Nucleotide sequence analysis revealed that P2/117 differs from the vaccine strain by just 23 point mutations. Three occur in the 5' noncoding region. The remainder result in a total of 5 coding changes located in VP1, VP4, 2B, and 3D. The likely role of these mutations in the evolution to neurovirulence is discussed.
Pollard
S R
SR
Department of Microbiology, University of Reading, England.
Dunn
G
G
Cammack
N
N
Minor
P D
PD
Almond
J W
JW
D000818:Animals; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics; D005814:Genes, Viral; D014644:Genetic Variation; D009154:Mutation; D009420:Nervous System / Q000382:microbiology; D017955:Poliovirus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D011055:Poliovirus Vaccine, Oral / Q000032:analysis*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D014774:Virulence
D004279:DNA, Viral; D011055:Poliovirus Vaccine, Oral; D012367:RNA, Viral