Clonal expansion of human T lymphocytes initiated by dendritic cells.
The accessory cell requirements for cloning T cells in the presence of lectin and T cell growth factors were examined with cells from human peripheral blood. We found that dendritic cells were active and perhaps essential. Single CD4+ lymphocytes could be cloned with 80% efficiency, and CD8+ cells with 50-60% efficiency if 10(3) syngeneic or allogeneic dendritic cells were added. Some T cell clones developed even with one dendritic cell. Monocytes or B lymphocytes from blood were at least 100-fold weaker in supporting clonal growth. These findings suggest a specialized feeder cell requirement, namely dendritic cells, for cloning T lymphocytes from single resting precursors.
Langhoff
E
E
Laboratory of Cellular Physiology and Immunology, Rockefeller University, New York, New York.
Steinman
R M
RM
D000938:Antigen-Presenting Cells / Q000166:cytology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D001402:B-Lymphocytes / Q000502:physiology; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology; D016827:CD8 Antigens; D002478:Cells, Cultured; D002999:Clone Cells; D003713:Dendritic Cells / Q000502:physiology*; D005434:Flow Cytometry; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D009000:Monocytes / Q000502:physiology; D013601:T-Lymphocytes / Q000166:cytology*
D000945:Antigens, Differentiation, T-Lymphocyte; D016827:CD8 Antigens; D007376:Interleukin-2