Structural inferences for the native skeletal muscle sodium channel as derived from patterns of endogenous proteolysis.
The alpha subunit (Mr approximately 260,000) of the rat skeletal muscle sodium channel is sensitive to cleavage by endogenous proteases during the isolation of muscle surface membrane. Antisera against synthetic oligopeptides were used to map the resultant fragments in order to identify protease-sensitive regions of the channel's structure in its native membrane environment. Antibodies to the amino terminus labeled major fragments of Mr approximately 130,000 and 90,000 and lesser amounts of other peptides as small as Mr approximately 12,000. Antisera to epitopes within the carboxyl-terminal half of the primary sequence recognized two fragments of Mr approximately 110,000 and 78,000. Individual antisera also selectively labeled smaller polypeptides in the most extensively cleaved preparations. The immunoreactivity patterns of monoclonal antibodies previously raised against the purified channel were then surveyed. The binding sites for one group of monoclonals, including several that recognize subtype-specific epitopes in the channel structure, were localized within a 12-kDa fragment near the amino terminus. The distribution of carbohydrate along the primary structure of the channel was also assessed by quantitating 125I-wheat germ agglutinin and 125I-concanavalin A binding to the proteolytic peptides. Most of the carbohydrate detected by these lectins was located between 22 and 90 kDa from the amino terminus of the protein. No lectin binding was detected to fragments arising from carboxyl-terminal half of the protein. These results were analyzed in terms of current models of sodium channel tertiary structure. In its normal membrane environment, the skeletal muscle sodium channel appears sensitive to cleavage by endogenous proteases in regions predicted to link the four repeat domains on the cytoplasmic side of the membrane while the repeat domains themselves are resistant to proteolysis.
Kraner
S
S
Mahoney Institute of Neurological Sciences, University of Pennsylvania School of Medicine, Philadelphia 19104.
Yang
J
J
Barchi
R
R
D000818:Animals; D000937:Antigen-Antibody Reactions; D015153:Blotting, Western; D006868:Hydrolysis; D007106:Immune Sera; D007457:Iodine Radioisotopes; D008297:Male; D008565:Membrane Proteins / Q000302:isolation & purification* / Q000378:metabolism; D008970:Molecular Weight; D009132:Muscles / Q000378:metabolism*; D010447:Peptide Hydrolases; D051381:Rats; D011919:Rats, Inbred Strains; D015222:Sodium Channels / Q000378:metabolism*; D013329:Structure-Activity Relationship; D014909:Wheat Germ Agglutinins / Q000378:metabolism
D007106:Immune Sera; D007457:Iodine Radioisotopes; D008565:Membrane Proteins; D015222:Sodium Channels; D014909:Wheat Germ Agglutinins; D010447:Peptide Hydrolases