[Physico-chemical properties of low density lipoproteins isolated in an equilibrium density gradient].
The tryptophanyls of total low density lipoproteins (LDL) (1.006-1.063 g/ml) from coronary heart disease (CHD) patients and subjects without CHD signs had different accessibility to fluorescence quenchers (I-and acrylamide). LDL were separated into subfractions in equilibrium density gradient. The coefficient of extinction , quantum yield and other spectral characteristics of LDL intrinsic fluorescence, rotational mobility of maleimide spin labels and fatty acid spin probe were different in LDL subfractions from healthy subjects. LDL subfractions with hydrated density 1.045-1.05 g/ml bound to B,E-receptors of cultured fibroblasts more effectively than did subfractions with density 1.01-1.03 g/ml. Structural differences of apo-B in the particles with different lipid to protein ratio are supposed.
Valentinova
N V
NV
Bushueva
T L
TL
Medvedeva
N V
NV
Morozkin
A D
AD
Fuki
I V
IV
D001055:Apolipoproteins B / Q000097:blood / Q000302:isolation & purification; D003327:Coronary Disease / Q000097:blood; D004578:Electron Spin Resonance Spectroscopy; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood* / Q000302:isolation & purification; D011956:Receptors, Cell Surface / Q000378:metabolism; D018110:Receptors, Lipoprotein; D013050:Spectrometry, Fluorescence
D001055:Apolipoproteins B; D008077:Lipoproteins, LDL; D011956:Receptors, Cell Surface; D018110:Receptors, Lipoprotein; C032531:apolipoprotein B,E receptor
Fiziko-khimicheskie svoĭstva podfraktsiĭ lipoproteidov nizkoĭ plotnosti, poluchennykh v ravnovesnom gradiente plotnosti.