A quantitative comparison of dual control of a hormone response element by progestins and glucocorticoids in the same cell line.
Progesterone receptor-containing T47D human breast cancer cells are responsive to progestins but fail to respond to other steroid hormones, in particular dexamethasone, because they have no measurable levels of receptors for estrogens, androgens, or glucocorticoids. To quantitatively study dual responsiveness of the mouse mammary tumor virus (MMTV) promoter to progestins and glucocorticoids, we have stably transfected T47D cells with a glucocorticoid receptor (GR) expression vector. A cloned derivative (A1-2) was isolated that expresses a normal, full length GR, as assessed by steroid binding and Western immunoblot with a monoclonal anti-GR antibody. Moreover, GR is expressed at levels (80,000-100,000 molecules per cell) comparable to the high levels of endogenous progesterone receptor (200,000 molecules per cell). In A1-2 cells transiently transfected with an MMTV-chloramphenicol acetyl transferase reporter gene, induction by glucocorticoid was substantially greater (5-fold) than induction mediated by progestins. These results suggest that glucocorticoids may be the primary regulator of MMTV.
Nordeen
S K
SK
Department of Pathology, University of Colorado Health Sciences Center, Denver 80262.
Kühnel
B
B
Lawler-Heavner
J
J
Barber
D A
DA
Edwards
D P
DP
D001943:Breast Neoplasms; D005938:Glucocorticoids / Q000502:physiology*; D006801:Humans; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics; D010957:Plasmids; D011374:Progesterone / Q000502:physiology; D011401:Promoter Regions, Genetic; D011965:Receptors, Glucocorticoid / Q000502:physiology*; D011980:Receptors, Progesterone / Q000502:physiology*; D012045:Regulatory Sequences, Nucleic Acid; D014162:Transfection; D014407:Tumor Cells, Cultured
D005938:Glucocorticoids; D011965:Receptors, Glucocorticoid; D011980:Receptors, Progesterone; D011374:Progesterone