title: Homologous potyvirus and flavivirus proteins belonging to a superfamily of helicase-like proteins.
abstract: Comparison of the nucleoside triphosphate-binding motif(NTBM)-containing proteins of two groups of apparently distantly related positive-strand RNA viruses (potyvirus and flavivirus), revealed significant sequence similarity. In addition, these two groups of viral proteins show amino acid motifs in common with those conserved in a group of five NTBM-containing proteins from prokaryotic and eukaryotic cells, some of which have been experimentally related to helicase activity. Here we propose that the proteins mentioned above constitute a superfamily of helicase-like proteins, distinct from the one previously described [Gorbalenya et al., FEBS Lett. 235 (1988) 16-24; Hodgman, Nature 333 (1988) 22-23; 578], which includes the NTBM-containing proteins from another group of positive-strand RNA viruses, the 'Sindbis-like' viruses.
authors.affiliation: Centro de Biología Molecular (CSIC-UAM), Universidad Autónoma, Madrid, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Laín
authors.forename: J L
authors.initials: JL
authors.lastname: Riechmann
authors.forename: M T
authors.initials: MT
authors.lastname: Martín
authors.forename: J A
authors.initials: JA
authors.lastname: García
mesh_terms: D000595:Amino Acid Sequence; D053487:DEAD-box RNA Helicases; D004338:Drug Combinations; D039601:Eukaryotic Initiation Factor-4A; D005416:Flavivirus / Q000235:genetics*; D005814:Genes, Viral; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics; D010448:Peptide Initiation Factors / Q000235:genetics; D010942:Plant Viruses / Q000235:genetics*; D011494:Protein Kinases; D020365:RNA Helicases; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000145:classification* / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics*
chemical_list: D004338:Drug Combinations; D009687:Nuclear Proteins; D010448:Peptide Initiation Factors; D014764:Viral Proteins; D015678:Viral Structural Proteins; D011494:Protein Kinases; D039601:Eukaryotic Initiation Factor-4A; C506749:Ddx5 protein, human; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases