An indirect immunofluorescence method for detection of infectious BK virus in urine.
An indirect immunofluorescence (IF) method is described for the detection of infectious BK virus in urine within seven days in contrast to up to three months or longer using routine tissue culture. Virus is pelleted from the urine, inoculated onto pre-formed monolayers of human embryo lung (HEL) fibroblasts, and infected cells are detected in an indirect fluorescent antibody test using a human serum. The sensitivity of the IF method is 91%. Positive isolates may be confirmed as BK virus using specific rabbit antisera on the original inoculated cultures. Furthermore, a virus stock may be grown up by passage from the original culture fluids for further studies such as DNA analysis. As a broadly-reactive human serum is used for screening the cultures, other viruses which grow in HEL cells, such as CMV, may also be detected.
Knowles
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Virus Reference Laboratory, Central Public Health Laboratory, London, U.K.
Woodroof
M
M
Porter
A A
AA
Gardner
S D
SD
D001739:BK Virus / Q000302:isolation & purification*; D005347:Fibroblasts; D005455:Fluorescent Antibody Technique; D006801:Humans; D011120:Polyomavirus / Q000302:isolation & purification*; D013997:Time Factors; D014556:Urine / Q000382:microbiology*