Determination of distances of sugar protons from Mn2+ in concanavalin A.
1H NMR spin-lattice relaxation time (T1) measurements have been carried out with various sugars, viz. methyl alpha-D-glucopyranoside (alpha-MeGluP), methyl beta-D-glucopyranoside (beta-MeGluP), methyl alpha-D-mannopyranoside (alpha-MeManP), maltose (4-O-alpha-D-glucopyranosyl-D-glucose), nigerose (3-O-alpha-D-glucopyranosyl-D-glucose), p-nitrophenyl alpha-maltoside (PNP-alpha-maltoside) and p-nitrophenyl beta-maltoside (PNP-beta-maltoside) to determine the distances of sugar protons from Mn2+ in concanavalin A (Con A). With a rotational correlation time of 1.58 x 10(-10) s determined, distances were calculated using Solomon-Bloembergen equation. The data obtained indicated differences in disposition of different groups in the binding site of Con A. An average value of about 10 A was obtained for the distances of sugar protons from Mn2+ in Con A. In the case of mono and disaccharides, the non-reducing end sugar unit was found to be closer to Mn2+ than the reducing end one.
Divakar
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D002241:Carbohydrates; D003208:Concanavalin A; D009682:Magnetic Resonance Spectroscopy; D008345:Manganese; D015394:Molecular Structure; D011522:Protons
D002241:Carbohydrates; D011522:Protons; D003208:Concanavalin A; D008345:Manganese