Control region of SV40 minichromosomes is preferentially cleaved by single-strand specific S1 nuclease.
We have analysed S1 sensitivity of SV40 minichromosomes isolated from the nuclei of infected cells at the late stage of infection. We show that a fraction of purified minichromosomes is sensitive towards double-strand cleavage by S1 nuclease. The pattern of specific cleavage reminiscent of that found for subcloned fragment under supercoiling is superimposed upon apparently random double-strand cuts along the entire regulatory region. Therefore, the cleavage sites are not exclusively confined to the regions with the reported alternate DNA conformation.
Nedospasov
S A
SA
Swiss Institute for Experimental Cancer Research, Epalinges.
Shakhov
A N
AN
Hirt
B
B
D004262:DNA Restriction Enzymes; D008968:Molecular Conformation; D015183:Restriction Mapping; D012303:Ring Chromosomes; D013539:Simian virus 40 / Q000235:genetics*; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism*
D004262:DNA Restriction Enzymes; D015719:Single-Strand Specific DNA and RNA Endonucleases