Structure and expression of a split chloroplast gene from mustard (Sinapis alba): ribosomal protein gene rps16 reveals unusual transcriptional features and complex RNA maturation.
The mustard chloroplast gene rps16 is split by an 887 bp group II (or III) intron. Three RNA 5' ends upstream of the rps16 coding region define both the transcription start site and two RNA processing sites. The DNA region preceding the start site contains a procaryotic-type "-10" promoter element, but not a typical "-35" element. One single RNA 3' end has been detected downstream from the rps16 coding region, but it is not in close proximity to any inverted repeat that might serve as a termination signal. Northern analysis has revealed several rps16 transcripts ranging in size from 1.6 kb to 0.5 kb. During seedling development, transcript levels show an initial increase and then remain constant without much difference between seedlings grown under light or in the dark.
Neuhaus
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Arbeitsgruppe Pflanzliche Zellphysiologie und Molekularbiologie, Ruhr-Universität Bochum, Federal Republic of Germany.
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D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D001937:Brassica / Q000235:genetics*; D002736:Chloroplasts / Q000502:physiology*; D004247:DNA / Q000235:genetics; D004720:Endonucleases; D005786:Gene Expression Regulation; D007438:Introns; D008969:Molecular Sequence Data; D009149:Mustard Plant / Q000235:genetics*; D009690:Nucleic Acid Conformation; D010946:Plants, Medicinal; D012335:RNA, Ribosomal / Q000235:genetics*; D012269:Ribosomal Proteins / Q000235:genetics*; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014158:Transcription, Genetic
D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; D004247:DNA; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases