Effects of co-culture with vascular endothelial cells on the renin-like enzyme production in vascular smooth muscle cells.
Cultured human vascular smooth muscle cells (VSMC) produced an immunologically specific renin-like enzyme. The production of the renin-like enzyme was increased by 1 mM N6-O2'-dibutyryladenosine 3',5' cyclic monophosphoric acid sodium salt (db-cAMP). When co-cultured with human vascular endothelial cells (EC) and human skin fibroblast (FB), the basal production of the renin-like enzyme was increased. And the extent of the increase by db-cAMP was amplified by co-culture with EC, but not by the co-culture with FB.
Iwai
N
N
Department of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.
Matsunaga
M
M
Kita
T
T
Kawai
C
C
D002478:Cells, Cultured; D000242:Cyclic AMP / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D012083:Renin / Q000096:biosynthesis*
D000242:Cyclic AMP; D012083:Renin