High affinity binding sites for basic fibroblast growth factor in rat hepatic plasma membranes.
The binding of [125I]-recombinant basic FGF (rec bFGF) to rat hepatic plasma membranes was investigated. [125I] rec bFGF bound to an apparent single class of high affinity binding sites (KD = 69 pM; Bmax = 9.61 fmoles/mg proteins). The absence of low affinity sites was confirmed by the inability of sulphated polysaccharides and heparinase to interfere with FGF binding. A good correlation existed between the ability of bovine pituitary-derived bFGF, rec bFGF and bovine brain-derived aFGF to displace [125I]rec bFGF from these binding sites and their in vitro potency on bovine aortic endothelial cell proliferation.
Gauthier
T
T
Sanofi Recherche - 195, Toulouse, France.
Herbert
J J
JJ
Maftoun
M
M
Picard
C
C
Morre
M
M
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002455:Cell Division; D002462:Cell Membrane / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology; D005260:Female; D005346:Fibroblast Growth Factors / Q000378:metabolism* / Q000494:pharmacology; D019763:Heparin Lyase; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D010902:Pituitary Gland / Q000032:analysis; D011133:Polysaccharide-Lyases / Q000494:pharmacology; D011134:Polysaccharides / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000378:metabolism*; D017468:Receptors, Fibroblast Growth Factor; D011994:Recombinant Proteins / Q000378:metabolism / Q000494:pharmacology
D011134:Polysaccharides; D011956:Receptors, Cell Surface; D017468:Receptors, Fibroblast Growth Factor; D011994:Recombinant Proteins; D005346:Fibroblast Growth Factors; D011133:Polysaccharide-Lyases; D019763:Heparin Lyase