title: A stopped-flow mixer device for a batch microcalorimeter application to NAD-NADase reaction.
abstract: A new molded polypropylene, diamond-like carbon (DLC)-coated mixing cell has been developed for use in the batch microcalorimeter. Reagent volume can be varied from 25 microliters to 100 microliters. A 10 microcalorie reaction heat can be measured to 5%. Repeat reactions can be done as often as every 10 min for a fast reaction. Reactions can be started within 1 h or less after loading. A pre-equilibrator and a temperature-controlled syringe drive unit permit solutions to be stored at 4 degrees C while being run at any temperature from -20 degrees C to 40 degrees C. The kinetics and enthalpy of reaction of NAD-NADase have been measured. delta H is about 21 kcal/mol endothermic.
authors.affiliation: National Heart, Lung, and Blood Institute, Laboratory of Technical Development, Bethesda, MD 20892.
authors.forename: R L
authors.initials: RL
authors.lastname: Berger
authors.forename: C P
authors.initials: CP
authors.lastname: Mudd
authors.forename: T
authors.initials: T
authors.lastname: Clem
authors.forename: T
authors.initials: T
authors.lastname: Kolobow
authors.forename: E
authors.initials: E
authors.lastname: Beile
authors.forename: P C
authors.initials: PC
authors.lastname: Simons
authors.forename: S
authors.initials: S
authors.lastname: Michel
authors.forename: W
authors.initials: W
authors.lastname: McClintock
mesh_terms: D002151:Calorimetry / Q000295:instrumentation / Q000379:methods; D007700:Kinetics; D008832:Microchemistry; D009243:NAD / Q000378:metabolism*; D009244:NAD+ Nucleosidase / Q000378:metabolism*
chemical_list: D009243:NAD; D009244:NAD+ Nucleosidase