Protein-DNA crosslinks induced by primary and secondary radicals.
Double-stranded DNA from calf thymus was irradiated in the presence of bovine serum albumin (BSA) at a ratio of 1:10 by mass under air, N2 or N2O, with and without ethanol, tert-butanol or isopropanol in 10(-2) mol dm-3 sodium phosphate buffer, pH 7.0. The irradiated solutions were analysed by a nitrocellulose filter assay and by high-performance liquid gel chromatography. The results show that OH. radicals as well as hydrated electrons induce protein-DNA crosslinks. Further, alcohol radicals react with BSA producing protein radicals which become crosslinked to DNA. If OH radicals are scavenged by alcohol, strand breakage of DNA does not occur.
Schuessler
H
H
Institut für Radiologie, Universität Erlangen-Nürnberg, F.R. Germany.
Jung
E
E
D000438:Alcohols; D003432:Cross-Linking Reagents; D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage; D004583:Electrons; D005609:Free Radicals; D006878:Hydroxides; D000860:Hypoxia; D066298:In Vitro Techniques; D009584:Nitrogen; D009609:Nitrous Oxide; D012710:Serum Albumin, Bovine; D013481:Superoxides
D000438:Alcohols; D003432:Cross-Linking Reagents; D005609:Free Radicals; D006878:Hydroxides; D013481:Superoxides; D012710:Serum Albumin, Bovine; D004247:DNA; D009609:Nitrous Oxide; D009584:Nitrogen