ATP synthesis coupled to methane formation from methyl-CoM and H2 catalyzed by vesicles of the methanogenic bacterial strain Gö1.
Methanogenesis from methyl-CoM and H2, as catalyzed by inside-out vesicle preparations of the methanogenenic bacterium strain Gö1, was associated with ATP synthesis. That this ATP synthesis proceeded via an uncoupler-sensitive transmembrane proton gradient was concluded from the following results: 1. Various inhibitors that affected methane formation (e.g. 2-bromomethanesulfonate) also prevented ATP synthesis. 2. The protonophore 3,5-di-tert-butyl-4-hydroxybenzylidenemalononitrile, in combination with the K+ ionophore valinomycin, inhibited ATP synthesis completely without affecting methanogenesis. 3. The ATP synthase inhibitor diethylstilbestrol inhibited ATP synthesis. 4. Addition of the detergent sulfobetaine inhibited both methane formation and ATP synthesis; the former but not the latter could be restored by adding titanium(III) citrate as electron donor. In addition it was shown that ATP synthesis could also be driven by transmembrane proton gradients artificially imposed on the vesicles. Furthermore net methanogenesis-dependent ATP formation was shown by measuring [32P]phosphate incorporation.
Peinemann
S
S
Institut für Mikrobiologie der Georg-August-Universität, Göttingen, FRG.
Blaut
M
M
Gottschalk
G
G
D025181:ATP Synthetase Complexes; D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D019605:Euryarchaeota / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008623:Mercaptoethanol / Q000031:analogs & derivatives*; D015080:Mesna / Q000031:analogs & derivatives* / Q000378:metabolism; D008697:Methane / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000378:metabolism; D009570:Nitriles / Q000494:pharmacology; D010710:Phosphates / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism
D009097:Multienzyme Complexes; D009570:Nitriles; D010710:Phosphates; C001784:SF 6847; C023349:methyl coenzyme M; D008623:Mercaptoethanol; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D010770:Phosphotransferases; D025181:ATP Synthetase Complexes; D015080:Mesna; D008697:Methane