Identification of insertion sequence element IS427 in pTiT37 plasmid DNA of an Agrobacterium tumefaciens T37 isolate.
The isolation and characterization of an insertion sequence (IS) element, IS427, from Agrobacterium tumefaciens T37 is described. IS427 is present in three nonidentical copies on the pTiT37 plasmid. The copy that was isolated through transposition on the entrapment vector pUCD800 contains at its ends a 16-bp imperfect inverted repeat and generates a 2-bp duplication of the target DNA. IS427 does not show homology with previously characterized IS elements of A. tumefaciens, based on hybridization experiments and/or sequence comparison.
De Meirsman
C
C
F. A. Janssens Memorial Laboratory for Genetics, University of Leuven,Heverlee, Belgium.
Croes
C
C
Desair
J
J
Verreth
C
C
Van Gool
A
A
Vanderleyden
J
J
D001412:Bacillus subtilis / Q000235:genetics; D001483:Base Sequence; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005798:Genes, Bacterial; D005809:Genes, Regulator; D008969:Molecular Sequence Data; D010957:Plasmids; D015183:Restriction Mapping; D012231:Rhizobium / Q000235:genetics*
D004251:DNA Transposable Elements; D004269:DNA, Bacterial