title: Role of peptidergic neurons in ocular herpes simplex infection in mice.
abstract: Peptide-containing nerve fibers (peptidergic fibers) abundantly innervate the mammalian cornea. We investigated their role in ocular herpes simplex infection in mice by using capsaicin, which causes degeneration and permanent loss of peptidergic neurons in neonates and temporary peptide depletion in adult animals. The corneas of neonatally denervated BALB/c mice were observed for capsaicin-induced keratitis at 11-14 wk of age and were then infected bilaterally with herpes simplex virus 1 (HSV-1); trigeminal (TG) ganglia were cocultivated 6 wk later to establish the rate of latent infection. We also applied capsaicin eye drops to adult BALB/c mice that had been infected with HSV-1 6 wk earlier, and measured viral shedding before, and 3 days and 2 months after, administration of capsaicin drops; TG ganglia of these animals were cocultivated at 3 days and 2 months after capsaicin application. Neurotrophic keratitis was found in 81% of neonatally denervated animals; mortality rate due to HSV-1 infection was reduced from 80% in the controls to 24% in the capsaicin-treated group, and recovery of latent virus by cocultivation was reduced by 50%. Viral shedding could not be produced by capsaicin eye drops in adult animals infected with HSV-1. However, recovery of latent virus was significantly reduced in TG ganglia sampled 3 days and 2 months after capsaicin drops were instilled. Our findings suggest 1) that peptidergic fibers play a crucial role in the establishment of trigeminal HSV-1 latency and 2) that reactivation of latently infected ganglia can be inhibited by topical capsaicin.
authors.affiliation: Howard Hughes Medical Institute, University of California, San Francisco 94143.
authors.forename: C P
authors.initials: CP
authors.lastname: Herbort
authors.forename: S S
authors.initials: SS
authors.lastname: Weissman
authors.forename: D G
authors.initials: DG
authors.lastname: Payan
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002211:Capsaicin / Q000494:pharmacology; D003315:Cornea / Q000187:drug effects / Q000294:innervation / Q000382:microbiology; D003714:Denervation; D005260:Female; D007635:Keratitis, Dendritic / Q000382:microbiology / Q000503:physiopathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009479:Neuropeptides / Q000502:physiology*; D018139:Simplexvirus / Q000302:isolation & purification
chemical_list: D009479:Neuropeptides; D002211:Capsaicin