The effects of tritium on embryo development: the embryotoxic effects of [3H]tryptophan.
The effects of tritiated amino acids on the development of the early mouse embryo in vitro are described. [3H]tryptophan was extremely toxic, compared with [3H]glutamic acid, and inhibited growth of the two-cell embryo to the blastocyst stage. The LD50 values for the two amino acids were, respectively, 1 and 296 kBq/ml of medium. The two-cell embryo was more sensitive to the irradiation from incorporated [3H]tryptophan is selectively incorporated into two chromosomal proteins in the two-cell embryo, and that those proteins were stage-specific markers of development.
Killen
H M
HM
Department of Biochemistry, Trinity College, Dublin, Republic of Ireland.
Carroll
J
J
D000367:Age Factors; D000818:Animals; D001755:Blastocyst / Q000528:radiation effects*; D002451:Cell Compartmentation; D002875:Chromosomes / Q000378:metabolism; D002970:Cleavage Stage, Ovum / Q000528:radiation effects*; D010064:Embryo Implantation; D005260:Female; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D066298:In Vitro Techniques; D007930:Leucine / Q000378:metabolism; D051379:Mice / Q000196:embryology*; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000096:biosynthesis; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D014316:Tritium / Q000633:toxicity*; D014364:Tryptophan / Q000378:metabolism*
D005971:Glutamates; D009687:Nuclear Proteins; D012333:RNA, Messenger; D014316:Tritium; D018698:Glutamic Acid; D014364:Tryptophan; D007930:Leucine