title: Identification, partial purification and characterization of high-molecular-weight gelatin-degrading metalloproteinases produced by a rat mammary carcinoma cell line.
abstract: BC1 rat mammary carcinoma cells were found to secrete a unique profile of metalloproteinases, distinguished by two gelatin-degrading metalloproteinases of Mr greater than 220.10(3) and Mr much greater than 220.10(3). These enzymes were each partially purified by gel-filtration chromatography, and inhibitor studies showed them to be metalloproteinases. Under conditions where denatured collagen types I, II, and V were completely degraded, native collagen types I, II, IV and V, fibronectin, fibrinogen, C1q, casein, and denatured transferrin were not degraded significantly by these enzymes. The relationship of these enzymes to other extracellular matrix-degrading metalloproteinases and their possible significance in tumour invasion and metastasis is discussed.
authors.affiliation: Department of Pathology, University of Sydney, Australia.
authors.forename: A
authors.initials: A
authors.lastname: Nethery
authors.forename: R L
authors.initials: RL
authors.lastname: O'Grady
mesh_terms: D000818:Animals; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D018093:Gelatinases; D007700:Kinetics; D008325:Mammary Neoplasms, Experimental / Q000201:enzymology*; D008666:Metalloendopeptidases / Q000302:isolation & purification* / Q000378:metabolism; D003012:Microbial Collagenase / Q000378:metabolism; D008970:Molecular Weight; D010434:Pepsin A / Q000302:isolation & purification* / Q000378:metabolism; D011480:Protease Inhibitors / Q000494:pharmacology; D051381:Rats; D013379:Substrate Specificity; D014462:Ultrafiltration
chemical_list: D011480:Protease Inhibitors; D010434:Pepsin A; D018093:Gelatinases; D008666:Metalloendopeptidases; D003012:Microbial Collagenase