Expression of the E. coli Lac Z gene from a defective HSV-1 vector in various human normal, cancer-prone and tumor cells.
Introducing foreign genetic material into human cells is essential for the elucidation of the function of various human genes and has potential use in the treatment of human diseases by gene therapy. In this study we demonstrate that a defective herpes simplex virus type 1 vector, pHSVlac, can effectively transfer and express the Escherichia coli Lac Z gene in a variety of exponential and quiescent human cells. The human cells tested included representative cells derived from cancer-prone patients that presumably have various DNA repair deficiencies.
Boothman
D A
DA
Harvard Medical School, Department of Biological Chemistry, Boston, MA 02115.
Geller
A I
AI
Pardee
A B
AB
D001426:Bacterial Proteins / Q000032:analysis*; D002478:Cells, Cultured; D004260:DNA Repair; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005696:Galactosidases / Q000032:analysis*; D015870:Gene Expression; D005798:Genes, Bacterial; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D018139:Simplexvirus / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured; D001616:beta-Galactosidase / Q000032:analysis*
D001426:Bacterial Proteins; D005696:Galactosidases; D001616:beta-Galactosidase