[Immunoenzyme analysis of adenovirus hexons. Indication and characteristics of rabbit and mouse antibodies against hexons].
An enzyme-linked immunosorbent assay (ELISA) was developed for analysis of rabbit and mouse IgG antibodies specific to adenoviral hexon. The anti-hexon antibodies were detected by capture with purified hexon coated onto polystyrene microtiter plates and visualizing them by respective anti-IgG horseradish peroxidase conjugates. In the sera from hyperimmunized rabbits and mice as well as in the mouse ascite fluids the ELISA procedure revealed primarily type-specific (epsilon) and genus-specific (alpha) antigenic determinants in hexon but not those of intermediate specificities.
Khil'ko
S N
SN
Kirasova
M A
MA
Isakova
V R
VR
Burgasova
M P
MP
Kiseleva
E K
EK
D000256:Adenoviridae / Q000276:immunology*; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D000259:Aviadenovirus / Q000276:immunology*; D002213:Capsid / Q000276:immunology; D036022:Capsid Proteins; D002642:Chick Embryo; D002645:Chickens; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D007124:Immunoenzyme Techniques; D051379:Mice; D011817:Rabbits
D000914:Antibodies, Viral; D036022:Capsid Proteins; C018315:hexon capsid protein, Adenovirus
Immunofermentnyĭ analiz geksona adenovirusov. Indikatsiia i kharakteristika krolich'ikh i myshinykh antitel protiv geksonov.