Rapid sampling of multiple enzyme reactions.
A simple method of initiating and sampling six simultaneous reactions was devised. A commercially available vial rack was fitted with a Plexiglas overlaying sheet to stabilize the vials for the addition and sampling procedures. Glass vials were routinely used because of their thermal conductivity advantages. Samples were added and removed, and the reactions were mixed with a multichannel pipet using every other channel. The data showing six simultaneous progress curves for the rapid inactivation of herpes simplex virus ribonucleotide reductase were presented and analyzed. In addition, the time course of 12 reactions catalyzed by varicella zoster virus thymidine kinase were assayed at one min intervals generating 96 data points within 8.5 min. A second experiment generated data points every 30 s for six simultaneous replicate thymidine kinase reactions. The ease of use and high reproducibility of the method are demonstrated by these data.
Spector
T
T
Wellcome Research Laboratories, Research Triangle Park, North Carolina 27709.
Harrington
J A
JA
D004789:Enzyme Activation; D004798:Enzymes / Q000032:analysis*; D006564:Herpesviridae / Q000201:enzymology; D014645:Herpesvirus 3, Human / Q000201:enzymology; D008828:Microbiological Techniques; D012264:Ribonucleotide Reductases / Q000032:analysis; D013937:Thymidine Kinase / Q000032:analysis
D004798:Enzymes; D012264:Ribonucleotide Reductases; D013937:Thymidine Kinase