Expression of (+)-3-PPP binding sites in the PC12 pheochromocytoma cell line.
Phencyclidine binds with high affinity to both PCP and sigma receptors. We investigated whether the clonal cell line PC12 expressed either of these receptors, and found that these cells contain a haloperidol-sensitive (+)-[3H]3-PPP binding site with a KD of 56 nM, but no PCP binding sites. The (+)3-PPP binding sites in PC12 cells displayed a reversed stereoselectivity for the benzomorphan opiates compared to CNS sigma receptors. Neither nerve growth factor nor sodium butyrate treatment affected the expression of either (+)-3-PPP or TCP binding sites in PC12 cells.
Yang
Z W
ZW
Developmental Neurobiology Program, University of Pittsburgh Center for Neuroscience, PA 15213.
Paleos
G A
GA
Byrd
J C
JC
D000310:Adrenal Gland Neoplasms / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D015259:Dopamine Agents / Q000378:metabolism*; D006801:Humans; D010673:Pheochromocytoma / Q000378:metabolism*; D010880:Piperidines / Q000378:metabolism*; D011869:Radioligand Assay; D011955:Receptors, Drug / Q000378:metabolism*
D015259:Dopamine Agents; D010880:Piperidines; D011955:Receptors, Drug; C026539:preclamol