[Changes of Golgi complex during the induced differentiation of human gastric adenocarcinoma cells in vitro].
Golgi complex is a central link related to each kind of organella in cellular metabolic process, its morphological changes are often concerned with the cell differentiation and functional state. The Golgi complexes in tumour cells have the characteristics of poor development and non-typical structure, and closely related to their pathological differential degree. For this reason, having based on appraising the differential effect of human gastric adenocarcinoma cell line MGc 80-3 induced by dBcAMP in vitro, we had made a systematic observation on the changes of Golgi complex during the malignant phenotypical reversion of MGc 80-3 cells, in order to inquire into the relationship between the structural and functional changes of Golgi complex and the malignant phenotypic reversion of cancer cells. It was revealed by ultrathin sectioning and freeze-etching electron microscopy that, in MGc 80-3 cells, there were a few Golgi complexes, their volume was small, the amounts of saccule were few, they arranged irregularly, expanded and inflated, and the intramembranous particles on saccule were rare and not well-distributed. It displayed a non-developmental and non-typical structural state of Golgi complex. But after induced treatment with dBcAMP, the Golgi complexes had grown in number and distributed concentrated, , their volume enlarged, the saccules increased and arranged regularly, and the intermembranous particles on saccules were plentiful and well-distributed. They had restored a well-developed and typical structure of Golgi complex similar to that of the primary culture cell of human normal gastric mucous membrane. It showed that Golgi complex had further changed into a quite developed state during the induced differentiation. This alteration not only inhibited the malignant secretory activity of gastric carcinoma cells but also played a certain regulative role in the changes of the surface components of cancer cells. The structural and functional changes of Golgi complex were considered to be an important expression in the malignant phenotypical reversion of cancer cells, it had an important influence on the differentiation of cancer cells from malignant to normal direction.
Li
Q F
QF
Wang
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DY
D000230:Adenocarcinoma / Q000473:pathology*; D003994:Bucladesine / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D006056:Golgi Apparatus / Q000648:ultrastructure*; D006801:Humans; D008854:Microscopy, Electron; D010641:Phenotype; D013274:Stomach Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured
D003994:Bucladesine