title: [Study of polymorphism in human rRNA gene clusters].
abstract: Human ribosomal DNA (rDNA) probe specific for the 3' end of the 28S rRNA gene was used for detecting standard restriction fragments' length polymorphism (RFLPs) in the non-transcribed spacer. The conditions for hybridization of rDNA probe which eliminate cross hybridization of parts of 28S rRNA gene were developed. A test for detecting incompletely restricted DNA was also developed which may be used in experiments for detecting new RFLPs. It was found that a set of standard RFLPs was identical in various human tissues for one individual. Frequency of standard RFLPs in the non-transcribed spacer of human rRNA gene clusters was calculated.
authors.forename: B V
authors.initials: BV
authors.lastname: Skriabin
authors.forename: V M
authors.initials: VM
authors.lastname: Gindilis
authors.forename: N S
authors.initials: NS
authors.lastname: Kupriianova
authors.forename: M Ia
authors.initials: MIa
authors.lastname: Timofeeva
mesh_terms: D001345:Autoradiography; D001483:Base Sequence; D003001:Cloning, Molecular; D004275:DNA, Ribosomal / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length; D012335:RNA, Ribosomal / Q000235:genetics*; D012339:RNA, Ribosomal, 28S / Q000235:genetics*
chemical_list: D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; D012339:RNA, Ribosomal, 28S
vernacular_title: Issledovanie polimorfizma v predelakh klastera genov rRNK cheloveka.