Stimulation of phosphoinositide signaling pathway in murine B lymphocytes by a novel guanosine analog, 7-thia-8-oxoguanosine.
A novel guanosine analog, 7-thia-8-oxoguanosine, is shown to induce proliferation in nude mouse splenic lymphocytes in vitro as measured by increased DNA synthesis. Determination of the levels of sn-1,2-diacylglycerol and inositol 1,4,5-trisphosphate in the 7-thia-8-oxoguanosine-treated B lymphocyte cultures revealed an enhanced formation of these second messengers leading to the activation and possible de novo synthesis of protein kinase C. The relevance of these results are discussed in regard to the transduction mechanism linking these guanosine analog generated signals with subsequent B lymphocyte activation events.
Parandoosh
Z
Z
Department of Molecular Pharmacology, ICN Nucleic Acid Research Institute, Costa Mesa, California 92626.
Ojo-Amaize
E
E
Robins
R K
RK
Jolley
W B
WB
Rubalcava
B
B
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects; D004075:Diglycerides / Q000378:metabolism; D005260:Female; D006151:Guanosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D015544:Inositol 1,4,5-Trisphosphate; D007295:Inositol Phosphates / Q000378:metabolism; D007700:Kinetics; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008815:Mice, Inbred Strains; D010716:Phosphatidylinositols / Q000502:physiology*; D011493:Protein Kinase C / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D013154:Spleen / Q000276:immunology
D004075:Diglycerides; D007295:Inositol Phosphates; D010716:Phosphatidylinositols; D006151:Guanosine; C061136:isatoribine; D015544:Inositol 1,4,5-Trisphosphate; D011493:Protein Kinase C