title: Presence of circulating anodic antigen in serum of Schistosoma intercalatum-infected patients from Gabon.
abstract: The presence of the schistosome circulating anodic antigen (CAA) in serum of Schistosoma intercalatum-infected patients from Gabon has been investigated using an enzyme-linked immunosorbent assay (ELISA). Blood samples were collected from 10 endemic controls, 29 patients which excreted viable S. intercalatum eggs in rectal mucosa and stool, six persons in which only non-viable eggs were found in rectal biopsy specimens and one person in which besides non-viable eggs a small number of viable eggs was found in the rectal biopsy specimen. CAA, a genus-specific antigen, could be demonstrated in 58.6% of the patients with S. intercalatum eggs in their stools. In comparison to S. mansoni infections, very light infections (0.6 eggs per gram faeces) could be detected by the ELISA. A strong correlation between parasite burden (eggs per gram faeces) and antigen-level (CAA-titer) was found (Spearman's rho = 0.65). Only one positive ELISA-results was found in the group with solely non-viable eggs in rectal tissue. As no false positive results were detected for the negative controls, the present results suggest, in accordance with results earlier obtained for schistosomiasis mansoni, that only in active S. intercalatum infections is antigen demonstrable.
authors.affiliation: Laboratory of Parasitology, Medical Faculty, State University of Leiden, The Netherlands.
authors.forename: N
authors.initials: N
authors.lastname: de Jonge
authors.forename: G
authors.initials: G
authors.lastname: Schommer
authors.forename: F W
authors.initials: FW
authors.lastname: Krijger
authors.forename: H
authors.initials: H
authors.lastname: Feldmeier
authors.forename: K
authors.initials: K
authors.lastname: Zwingenberger
authors.forename: A
authors.initials: A
authors.lastname: Steiner
authors.forename: U
authors.initials: U
authors.lastname: Bienzle
authors.forename: A M
authors.initials: AM
authors.lastname: Deelder
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology; D005681:Gabon; D006801:Humans; D010270:Parasite Egg Count; D011237:Predictive Value of Tests; D012547:Schistosoma / Q000276:immunology*; D012552:Schistosomiasis / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000947:Antigens, Helminth