title: Different binding to DNA of enantiomeric platinum complexes assessed by inhibition of restriction enzyme activity.
abstract: The ability of enantiomeric platinum complexes to block the action of selected restriction enzymes has been investigated. The complexes [PtCl2(DAC)], [PtCl2(DAB)] and [PtCl2(DAP)] (DAC = 1,2-diamminocyclohexane; DAB = 2,3-diamminobutane; DAP = 1,2-diamminopropane) exhibit a guanine-cytosine preference in accord with previous results on cis-[PtCl2(NH3)2] (cis-DDP). The extent of inhibition, however, is significantly different for the different isomers; the R,R form is more active than the others at short incubation time, as the time becomes longer, the differences among isomers level off. It also appears that cis-DDP is more active than [PtCl2(DAC)] in blocking the Cfo I enzyme, though it shows a preference for the G-X-G sequences.
authors.affiliation: Istituto di Patologia Generale, Bari, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Coluccia
authors.forename: D
authors.initials: D
authors.lastname: Giordano
authors.forename: F
authors.initials: F
authors.lastname: Loseto
authors.forename: F P
authors.initials: FP
authors.lastname: Intini
authors.forename: L
authors.initials: L
authors.lastname: Maresca
authors.forename: G
authors.initials: G
authors.lastname: Natile
mesh_terms: D000970:Antineoplastic Agents / Q000378:metabolism*; D002945:Cisplatin / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D004262:DNA Restriction Enzymes / Q000037:antagonists & inhibitors*; D009944:Organoplatinum Compounds / Q000378:metabolism; D010984:Platinum / Q000378:metabolism* / Q000494:pharmacology; D013237:Stereoisomerism
chemical_list: D000970:Antineoplastic Agents; D009944:Organoplatinum Compounds; D010984:Platinum; C013933:dichloro-1,2-diaminocyclohexane platinum complex; D004247:DNA; D004262:DNA Restriction Enzymes; D002945:Cisplatin