Disparate effects of two herpesvirus [corrected] immediate-early gene trans-activators on the HIV-1 LTR.
The BMLF1 region of the Epstein-Barr virus (EBV) genome and the immediate-early (IE) region of human cytomegalovirus (HCMV) both encode proteins which can trans-activate heterologous promoter/chloramphenicol acetyl transferase (CAT) constructs, including a human immunodeficiency virus type-1 promoter/CAT construct. We demonstrate here that this trans-activation by the EBV BMLF1 gene product, which we have previously shown to be largely post-transcriptional, is reporter gene dependent. In contrast, trans-activation by the HCMV-IE gene product(s), previously shown to be mediated at the RNA level, is seen regardless of whether CAT, human growth hormone, or beta-galactosidase is used as the reporter gene. Mutational analysis revealed no specific cis-acting sequences within the HIV-1 promoter which were required for trans-activation by the HCMV-IE gene product(s).
Markovitz
D M
DM
Department of Medicine, University of North Carolina, Chapel Hill 27599.
Kenney
S
S
Kamine
J
J
Smith
M S
MS
Davis
M
M
Huang
E S
ES
Rosen
C
C
Pagano
J S
JS
D002277:Carcinoma; D003110:Colonic Neoplasms; D003587:Cytomegalovirus / Q000235:genetics*; D004252:DNA Mutational Analysis; D015497:HIV-1 / Q000235:genetics*; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D009687:Nuclear Proteins / Q000235:genetics; D011401:Promoter Regions, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D015534:Trans-Activators / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured
D009687:Nuclear Proteins; D015534:Trans-Activators