[Analysis of P-glycoprotein in patients with acute leukemias by flow cytometry].
The identification of a P-glycoprotein product of multidrug-resistant gene (mdr 1) was reported recently. To examine the expression of the P-glycoprotein in acute leukemias of various types, we have prepared leukemic blast cells from patients and measured their positivity of P-glycoprotein using monoclonal anti-P-glycoprotein antibody (C219) by flow cytometry. P-glycoprotein is expressed in 8 out of 44 cases including leukemic blast cells but not lymphocytes and monocytes. In these cases showed drug resistance was shown clinically. In addition, the expression of the P-glycoprotein was not observed in the drug-sensitive cases and at the time of initial chemotherapy. Our results suggest that measurement of P-glycoprotein in acute leukemias by flow cytometry may prove to be a valuable tool for the design of chemotherapy protocols.
Funato
T
T
Bando
Y
Y
Kato
H
H
Tokuhiro
H
H
Shimoda
Y
Y
Ohtani
H
H
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000208:Acute Disease; D004351:Drug Resistance / Q000235:genetics; D005434:Flow Cytometry; D015870:Gene Expression; D006801:Humans; D007938:Leukemia / Q000097:blood* / Q000235:genetics; D008562:Membrane Glycoproteins / Q000097:blood* / Q000235:genetics
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D008562:Membrane Glycoproteins