Mechanisms of pancreatic beta-cell growth and regeneration: studies on rat insulinoma cells.
Information about the mechanism of beta-cell growth and regeneration may be obtained by studies of insulinoma cells. In the present study the growth and function of the rat insulinoma cell lines RINm5F and 5AH were evaluated by addition of serum, hormones, and growth factors. It was found that transferrin is the only obligatory factor whereas growth hormone, epidermal growth factor, fibroblast growth factor, and TRH had modulating effects. A heat-labile heparin binding serum factor which stimulated thymidine incorporation but not cell proliferation was demonstrated in human serum. Measurements of insulin mRNA content showed that the insulinoma cells only contained about 2% of that of normal rat beta-cells. These results are discussed in relation to the role of growth factors, oncogenes, and differentiation in the growth and regeneration of beta-cells.
Nielsen
J H
JH
Hagedorn Research Laboratory, Gentofte, Denmark.
D007516:Adenoma, Islet Cell / Q000473:pathology*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D013006:Growth Hormone / Q000494:pharmacology; D007328:Insulin / Q000235:genetics / Q000378:metabolism; D007340:Insulinoma / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D007515:Islets of Langerhans / Q000166:cytology / Q000473:pathology* / Q000502:physiology; D010179:Pancreas / Q000032:analysis / Q000187:drug effects / Q000378:metabolism; D010190:Pancreatic Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D012038:Regeneration; D013936:Thymidine / Q000378:metabolism; D013973:Thyrotropin-Releasing Hormone / Q000494:pharmacology; D014168:Transferrin / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology
D007328:Insulin; D012333:RNA, Messenger; D014168:Transferrin; D013973:Thyrotropin-Releasing Hormone; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor; D013006:Growth Hormone; D013936:Thymidine