title: Dehydroepiandrosterone and related steroids inhibit mitochondrial respiration in vitro.
abstract: 1. Dehydroepiandrosterone (DHEA) inhibited mitochondrial respiratory rates in the presence of tricarboxylic acid cycle intermediates with the exception of succinate. 2. Several other steroids tested for inhibitory activity on mitochondrial state 3 rate showed inhibitory potencies ranging from 0-90%. 3. Mitochondria isolated from adrenals, heart, kidneys, brain and brown adipose tissue were also inhibited by DHEA. 4. The aspartate malate shuttle system was inhibited, but the alpha-glycerophosphate shuttle was totally insensitive to DHEA. 5. The high-amplitude swelling of mitochondria in hypotonic media was inhibited by DHEA and a few other steroids.
authors.affiliation: Hormel Institute, University of Minnesota, Austin 55912.
authors.forename: P F
authors.initials: PF
authors.lastname: Mohan
authors.forename: M P
authors.initials: MP
authors.lastname: Cleary
mesh_terms: D000818:Animals; D002951:Citrates / Q000378:metabolism; D019343:Citric Acid; D003687:Dehydroepiandrosterone / Q000494:pharmacology*; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D066298:In Vitro Techniques; D007656:Ketoglutaric Acids / Q000378:metabolism; D008293:Malates / Q000378:metabolism; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D008933:Mitochondrial Swelling / Q000187:drug effects; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats; D011924:Rats, Zucker; D013256:Steroids / Q000494:pharmacology*; D013386:Succinates / Q000378:metabolism; D019802:Succinic Acid
chemical_list: D002951:Citrates; D005971:Glutamates; D007656:Ketoglutaric Acids; D008293:Malates; D013256:Steroids; D013386:Succinates; D019343:Citric Acid; D018698:Glutamic Acid; D003687:Dehydroepiandrosterone; C030298:malic acid; D019802:Succinic Acid