Somatostatin increases intracellular Ca2+ concentration in cultured rat hippocampal neurons.
Changes of intracellular Ca2+ concentration [( Ca2+]i) in response to somatostatin were measured in cultured rat hippocampal neurons on a single cell basis by fura-2 fluorometry. Somatostatin increased [Ca2+]i dose-dependently and this effect was completely blocked in either Ca2+-depleted medium or LaCl3-containing medium. In addition, omega-conotoxin GVIA completely inhibited the effect of somatostatin. Our results indicate that somatostatin receptors couple with N-type voltage-sensitive Ca2+ channels in cultured rat hippocampal neurons.
Miyoshi
R
R
Third Department of Internal Medicine, Hiroshima University School of Medicine, Japan.
Kito
S
S
Katayama
S
S
Kim
S U
SU
D000818:Animals; D001572:Benzofurans; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D005456:Fluorescent Dyes; D016257:Fura-2; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013004:Somatostatin / Q000494:pharmacology*
D001572:Benzofurans; D005456:Fluorescent Dyes; D013004:Somatostatin; D002118:Calcium; D016257:Fura-2