Further studies on the identification of microglia in mixed brain cell cultures.
The investigation of brain microglia in primary cultures of dissociated cerebral cortical cells has been continued here utilizing a histochemical procedure for thiamine pyrophosphatase, which selectively stains microglia in whole tissue. This procedure also selectively stained a subpopulation of cells in the cortical cultures. The stained cells were small and exhibited quite variable morphology; from these features, a corresponding cell population could be identified in viable cultures. The stained cells, which were distinct from previously identified macrophage forms, were also distinguished from similar appearing cell types--small neurons and oligodendrocytes. Based on their staining properties, morphology, and distinction from other cell types, the identified cells are proposed as equivalents of ramified microglia.
Glenn
J A
JA
Department of Oral Biology, College of Dentistry, Ohio State University, Columbus 43210.
Jordan
F L
FL
Thomas
W E
WE
D000818:Animals; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology*; D004622:Embryo, Mammalian; D006651:Histocytochemistry; D009457:Neuroglia / Q000166:cytology* / Q000201:enzymology; D011755:Pyrophosphatases / Q000378:metabolism*; D051381:Rats; D013834:Thiamine Pyrophosphatase / Q000378:metabolism*
D011755:Pyrophosphatases; D013834:Thiamine Pyrophosphatase