title: Pathogenesis of Marek's disease virus-induced local lesions. 1. Lesion characterization and cell line establishment.
abstract: Subcutaneous (wing-web) or intramuscular inoculation of chickens with allogeneic normal or Marek's disease virus (MDV)-infected chicken kidney cells induced local lesions visible by 3-4 days postinoculation (PI). Lesions were slightly larger (P less than 0.05) in infected than uninfected chickens 5 and 8 days PI. They persisted and grew past 9 days PI only when infected. Infiltrating lymphocytes in infected and uninfected early lesions were similar; they included B-cells and also T-cells with and without Ia antigen. Up to 42% of lymphocytes from infected or uninfected lesions had the surface antigen MATSA. At 3 to 6 days PI, infected lesions contained lymphocytes with viral internal antigen, especially in Ia-bearing cells and MATSA-bearing cells, but thereafter infection was latent. Cells harvested daily from local lesions induced with allogeneic MDV-infected cells were cultured; MD tumor cell lines were established from lesions as early as 4 days PI, with a total success rate of about 50% thereafter. Either transformed tumor cells were already present during the early cytolytic infection period or else appropriate target cells were present that became infected in vivo and/or in vitro and then became transformed in vitro.
authors.affiliation: Department of Avian and Aquatic Animal Medicine, Cornell University, Ithaca, New York 14853.
authors.forename: B W
authors.initials: BW
authors.lastname: Calnek
authors.forename: B
authors.initials: B
authors.lastname: Lucio
authors.forename: K A
authors.initials: KA
authors.lastname: Schat
authors.forename: H S
authors.initials: HS
authors.lastname: Lillehoj
mesh_terms: D000818:Animals; D000957:Antigens, Viral, Tumor / Q000032:analysis; D002461:Cell Line, Transformed; D002645:Chickens / Q000382:microbiology*; D008381:Herpesvirus 2, Gallid / Q000276:immunology / Q000502:physiology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology* / Q000382:microbiology; D008380:Marek Disease / Q000209:etiology* / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D008854:Microscopy, Electron; D013601:T-Lymphocytes / Q000276:immunology; D013997:Time Factors
chemical_list: D000957:Antigens, Viral, Tumor