Effect of 3,4-dihydroxybutyl-1-phosphonate on cardiolipin synthesis in B. subtilis.
Endogenous phosphatidylglycerol is rapidly transformed into cardiolipin when B. subtilis 168 cells were incubated in a buffer without an energy source. Upon addition of 3,4-dihydroxybutyl-1-phosphonate (DHBP), a synthetic glycerol 3-phosphate analogue, this synthesis was completely blocked after a short lag; if the cells were grown in the presence of the analogue, there was no lag. When membrane fractions were incubated with exogenous [32P]phosphatidylglycerol, free DHBP and glycerol 3-phosphate had no effect on [32P]cardiolipin synthesis, but phosphatidyl-DHBP and phosphatidylglycerolphosphate were potent inhibitors. These results are consistent with our hypothesis that phosphatidylglycerolphosphate, the phosphatidylglycerol precursor, might also be a physical inhibitor of cardiolipin synthesis.
Lacombe
C
C
Laboratoire de Biochimie Cellulaire, UFR Sciences, Poitiers, France.
Lubochinsky
B
B
D001412:Bacillus subtilis / Q000187:drug effects* / Q000378:metabolism; D002072:Butylene Glycols / Q000494:pharmacology*; D002308:Cardiolipins / Q000096:biosynthesis*; D008565:Membrane Proteins; D063065:Organophosphonates; D010715:Phosphatidylglycerols / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism; D017855:Transferases (Other Substituted Phosphate Groups)
D002072:Butylene Glycols; D002308:Cardiolipins; D008565:Membrane Proteins; D063065:Organophosphonates; D010715:Phosphatidylglycerols; C034358:phosphatidylglycerophosphate; C013493:3,4-dihydroxybutyl-1-phosphonic acid; D010770:Phosphotransferases; D017855:Transferases (Other Substituted Phosphate Groups); C100091:cardiolipin synthetase