Effects of fructose 1,6-diphosphate on splanchnic artery occlusion shock in the rat.
Splanchnic artery occlusion (SAO) shock, produced by clamping splanchnic arteries for 45 min followed by the release of occlusion, was induced in male rats, treated 15 min before surgery, with fructose 1,6-diphosphate (FDP) or with equivalent doses of fructose or inorganic phosphate. Survival rate, peritoneal macrophage phagocytosis and plasma levels of myocardial depressant factor (MDF) were measured. Shocked animals pretreated with vehicle exhibited 24.6 +/- 0.9% phagocytic activity, 110 +/- 3.9 units/ml MDF plasma levels and 0% survival. Sham animals showed the following values: survival 100%; phagocytosis, 49.5 +/- 1.3%; MDF, 22 +/- 2.9 units/ml. Pretreatment with FDP (25 mg/kg/i.v.) significantly improved survival rate (50%) and macrophage phagocytosis (37.9 +/- 0.4%) and reduced plasma MDF levels (77 +/- 3 units/ml). Equivalent doses of fructose and inorganic phosphate did not improve survival, as well as lower doses of FDP. These results suggest a beneficial effect of FDP in SAO shock.
Squadrito
F
F
Institutes of Pharmacology and Microbiology, School of Medicine, University of Messina, Italy.
Sturniolo
R
R
Altavilla
D
D
Campo
G M
GM
Trimarchi
G R
GR
Scuri
R
R
Caputi
A P
AP
D000818:Animals; D003250:Constriction; D005635:Fructosediphosphates / Q000627:therapeutic use*; D006598:Hexosediphosphates / Q000627:therapeutic use*; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008264:Macrophages / Q000187:drug effects; D008297:Male; D009201:Myocardial Depressant Factor / Q000097:blood; D010587:Phagocytosis / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D012769:Shock / Q000188:drug therapy* / Q000209:etiology; D013152:Splanchnic Circulation
D005635:Fructosediphosphates; D006598:Hexosediphosphates; D007773:Lactates; D009201:Myocardial Depressant Factor; D019344:Lactic Acid; C029063:fructose-1,6-diphosphate