Cyclic AMP, fructose-2,6-bisphosphate and catabolite inactivation of enzymes in the hydrocarbon-assimilating yeast Candida maltosa.
The inactivation of fructose-1,6-bisphosphatase, isocitrate lyase and cytoplasmic malate dehydrogenase in Candida maltosa was found to occur after the addition of glucose to starved cells. The concentration of cyclic AMP and fructose-2,6-bisphosphate increased drastically within 30 s when glucose was added to the intact cells of this yeast. From these results it was concluded that catabolite inactivation, with participation of cyclic AMP and fructose-2,6-bisphosphate, is an important control mechanism of the gluconeogenetic sequence in the n-alkane-assimilating yeast Candida maltosa, as described for Saccharomyces cerevisiae.
Polnisch
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Sektion Biologie, Ernst-Moritz-Arndt-Universität Greifswald, German Democratic Republic.
Hofmann
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K
D000293:Adolescent; D000328:Adult; D002175:Candida / Q000187:drug effects / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism*; D005246:Feedback; D006597:Fructose-Bisphosphatase / Q000037:antagonists & inhibitors*; D005635:Fructosediphosphates / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology*; D006598:Hexosediphosphates / Q000378:metabolism*; D006801:Humans; D006838:Hydrocarbons / Q000378:metabolism; D007522:Isocitrate Lyase / Q000037:antagonists & inhibitors*; D007700:Kinetics; D008291:Malate Dehydrogenase / Q000037:antagonists & inhibitors*; D007652:Oxo-Acid-Lyases / Q000037:antagonists & inhibitors*
D005635:Fructosediphosphates; D006598:Hexosediphosphates; D006838:Hydrocarbons; C027652:fructose 2,6-diphosphate; D000242:Cyclic AMP; D008291:Malate Dehydrogenase; D006597:Fructose-Bisphosphatase; D007652:Oxo-Acid-Lyases; D007522:Isocitrate Lyase; D005947:Glucose