Acid stability of hepatitis A virus.
The acid stability of unpurified and highly purified hepatitis A virus (HAV) was tested and compared with that of poliovirus type 1, coxsackievirus types A9 and B1 and echovirus type 9. Only HAV had a high residual infectivity after 2 h of exposure to pH 1 at room temperature, remaining infectious for up to 5 h. At 38 degrees C, pH 1, HAV remained infectious for 90 min. Highly purified HAV was found to be infectious for 8 h at pH 1 and room temperature. This indicates that the increased stability is not due to protection by cellular material attached to the virus, but is a virus-specific marker. Under the same conditions, at pH 1 and room temperature, unpurified and highly purified HAV antigens were traceable for 5 and 4 h respectively.
Scholz
E
E
Abteilung für Medizinische Virologie und Epidemiologie der Viruskrankheiten, Hygiene-Institut der Universität Tübingen, F.R.G.
Heinricy
U
U
Flehmig
B
B
D002460:Cell Line; D004459:Echovirus 9 / Q000472:pathogenicity / Q000502:physiology; D004770:Enterovirus / Q000472:pathogenicity / Q000502:physiology; D029822:Enterovirus B, Human / Q000472:pathogenicity / Q000502:physiology; D006507:Hepatovirus / Q000472:pathogenicity* / Q000502:physiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009500:Neutralization Tests; D017955:Poliovirus / Q000472:pathogenicity / Q000502:physiology; D013045:Species Specificity; D014779:Virus Replication