Determination of rotavirus serotype-specific antibodies in sera by competitive enhanced enzyme immunoassay.
A method is described for the specific detection of antibody to individual rotavirus serotypes in sera. A competitive enzyme immunoassay (EIA) was developed in which rotavirus serotype-specific monoclonal antibodies against VP7 compete with antibodies in test sera for rotavirus serotype-specific antigen bound to a solid phase. There was an excellent correlation between serotype-specific EIA results and serotype-specific neutralization titres (r = 0.915, P = less than 0.001). The value of this method for rotavirus epidemiology and vaccine trials is discussed.
Beards
G M
GM
Regional Virus Laboratory, East Birmingham Hospital, U.K.
Desselberger
U
U
D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000032:analysis* / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D001667:Binding, Competitive; D002213:Capsid / Q000276:immunology; D036022:Capsid Proteins; D002675:Child, Preschool; D005069:Evaluation Studies as Topic; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D007231:Infant, Newborn; D009249:NADP; D009500:Neutralization Tests; D012401:Rotavirus / Q000145:classification / Q000276:immunology*; D012703:Serotyping; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; C050779:VP7 protein, Rotavirus; D009249:NADP