The products of the reduction of doxyl stearates in cells are hydroxylamines as shown by oxidation by 15N-perdeuterated Tempone.
The use of nitroxides in functional biological systems has increased greatly as it has become evident that such studies can provide valuable biophysical and metabolic data. This has led to a need to understand the nature of the metabolism of nitroxides and their products. This paper presents data indicating the value of 15N-perdeuterated Tempone specifically to indicate the amount of hydroxylamines that are present in a cellular system. Using this technique, we found that in the mammalian cells that we studied the principal or only products of reduction of doxyl stearates were the corresponding hydroxylamines.
Chen
K
K
Department of Physiology and Biophysics College of Medicine, University of Illinois, Urbana.
Swartz
H M
HM
D000818:Animals; D001853:Bone Marrow / Q000032:analysis; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003497:Cyclic N-Oxides / Q000378:metabolism*; D003903:Deuterium; D004578:Electron Spin Resonance Spectroscopy; D006898:Hydroxylamines / Q000032:analysis*; D051379:Mice; D010084:Oxidation-Reduction; D013950:Thymus Gland / Q000032:analysis / Q000166:cytology; D014216:Triacetoneamine-N-Oxyl
D003497:Cyclic N-Oxides; D006898:Hydroxylamines; D014216:Triacetoneamine-N-Oxyl; C044761:10-doxylstearic acid; C032363:16-nitroxystearic acid; D003903:Deuterium