title: Distribution of adenosine deaminase complexing protein (ADCP) in human tissues.
abstract: The normal distribution of adenosine deaminase complexing protein (ADCP) in the human body was investigated quantitatively by ADCP-specific radioimmunoassay (RIA) and qualitatively by immunohistochemistry. In these studies we used a specific rabbit anti-human ADCP antiserum. In all 19 investigated tissues, except erythrocytes, ADCP was found by RIA in the soluble and membrane fractions. From all tissues the membrane fractions contained more ADCP (expressed per mg protein) than the soluble fractions. High membrane ADCP concentrations were found in skin, renal cortex, gastrointestinal tract, and prostate. Immunoperoxidase staining confirmed the predominant membrane-associated localization of the protein. In serous sweat glands, convoluted tubules of renal cortex, bile canaliculi, gastrointestinal tract, lung, pancreas, prostate gland, salivary gland, gallbladder, mammary gland, and uterus, ADCP immunoreactivity was found confined to the luminal membranes of the epithelial cells. These data demonstrate that ADCP is present predominantly in exocrine glands and absorptive epithelia. The localization of ADCP at the secretory or absorptive apex of the cells suggests that the function of ADCP is related to the secretory and/or absorptive process.
authors.affiliation: Department of Pathology, University of Limburg, The Netherlands.
authors.forename: W N
authors.initials: WN
authors.lastname: Dinjens
authors.forename: J
authors.initials: J
authors.lastname: ten Kate
authors.forename: E P
authors.initials: EP
authors.lastname: van der Linden
authors.forename: J T
authors.initials: JT
authors.lastname: Wijnen
authors.forename: P M
authors.initials: PM
authors.lastname: Khan
authors.forename: F T
authors.initials: FT
authors.lastname: Bosman
mesh_terms: D000243:Adenosine Deaminase / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology; D018819:Dipeptidyl Peptidase 4; D006801:Humans; D007124:Immunoenzyme Techniques; D009700:Nucleoside Deaminases / Q000378:metabolism*; D011863:Radioimmunoassay; D012995:Solubility; D014018:Tissue Distribution
chemical_list: D002352:Carrier Proteins; D018819:Dipeptidyl Peptidase 4; D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase