Luminol dependent chemiluminescence of quartz and zymosan stimulated neutrophils.
The effect of the opsonization by zymosan and quartz particles on the chemiluminescence was investigated on human neutrophil granulocytes. Opsonization of zymosan enhanced the chemiluminescence response, while opsonized quartz inhibited the chemiluminescence reaction. Calcium ionophore A 23187 treatment did not influence the chemiluminescence of quartz but the light signal in the presence of quartz decreased rapidly. In parallel experiments the protein pattern of zymosan treated neutrophils was investigated by high resolution two-dimensional polyacrylamide gel electrophoresis.
Liszt
F
F
Department of Clinical Chemistry, University Medical School of Pécs, Hungary.
Jobst
K
K
D001798:Blood Proteins / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D008165:Luminol; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D009895:Opsonin Proteins; D010100:Oxygen / Q000378:metabolism; D010587:Phagocytosis; D011791:Quartz / Q000494:pharmacology*; D012822:Silicon Dioxide / Q000494:pharmacology*; D015054:Zymosan / Q000494:pharmacology*
D001798:Blood Proteins; D009895:Opsonin Proteins; D011791:Quartz; D008165:Luminol; D012822:Silicon Dioxide; D015054:Zymosan; D010100:Oxygen