Immunodetection of Na,K-ATPase alpha 3-isoform in renal and nerve tissues.
At least three types of mRNA of the catalytic subunit of Na,K-ATPase namely alpha-,alpha+- and alpha 3-isoforms are identified in different tissues. Only two of them alpha and alpha+ have well known structural and catalytic properties. Here we present immunochemical data indicating that the alpha 3 protein really exists in pig and human kidney, and human brain. Crude membrane fractions and purified membrane-bound Na,K-ATPases were immunoblotted with alpha 3-specific antibodies raised against the synthetic peptide corresponding to the unique sequence of this isoform. The mature alpha 3-subunit is shown to include the sequence GDKKDDKSSPK followed by the initiating methionine residue. Nephron collecting tubules are proposed to specifically contain Na,K-ATPase alpha 3-isoform.
Arystarkhova
E A
EA
Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, Moscow.
Lakhtina
O E
OE
Modyanov
N N
NN
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology*; D008861:Microsomes / Q000378:metabolism*; D008969:Molecular Sequence Data; D009417:Nerve Tissue / Q000201:enzymology*; D012333:RNA, Messenger / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013552:Swine
D007527:Isoenzymes; D012333:RNA, Messenger; D000254:Sodium-Potassium-Exchanging ATPase