Characteristics of collagenase in experimental periapical granulomas from the teeth of dogs.
Periapical granulomas were induced, with a success rate of about 60% (66 granulomas produced out of 109 roots treated) in mandibular premolars. The average wet weight of the granulomas, 46.1 +/- 34.5 mg (mean +/- SD, n = 22), was sufficient to allow individual specimens to be used for most of the biochemical analyses. High collagenase activity was extracted directly from the granulomas with 4 M urea solution. The enzyme was a typical animal collagenase (EC 3.4.24.7) which clove native collagen molecules into three-quarter (alpha A) and one-quarter (alpha B) length fragments. The collagenase was activated by 1 mM p-aminophenylmercuric acetate. This activated enzyme broke down collagen I rather than collagen III preferentially, which is similar to the activity of human polymorphonuclear leucocyte collagenase. The molecular weights of the latent and activated collagenases were 67 and 49 K, respectively.
Asai
K
K
Department of Endodontics, School of Dentistry, Aichi Gakiun University, Nagoya, Japan.
Kishi
J
J
Nakamura
H
H
Hayakawa
T
T
D000818:Animals; D002850:Chromatography, Gel; D003094:Collagen / Q000378:metabolism; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D003012:Microbial Collagenase / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D010484:Periapical Granuloma / Q000201:enzymology* / Q000473:pathology; D012967:Sodium Dodecyl Sulfate; D013045:Species Specificity
D012967:Sodium Dodecyl Sulfate; D003094:Collagen; D003012:Microbial Collagenase