title: Expression of type IV collagenase and procollagen genes and its correlation with the tumorigenic, invasive, and metastatic abilities of oncogene-transformed human bronchial epithelial cells.
abstract: In a series of immortalized human bronchial epithelial cell lines continuing various oncogenes, the transcriptional levels of the type IV collagenase and the type IV procollagen genes were compared with the properties of invasiveness in vitro and tumorigenicity and metastatic ability in athymic nude mice. v-Ha-ras greatly enhanced invasion and metastasis, whereas v-Ki-ras, c-myc, and c-raf had lesser effects on these malignant phenotypes. In addition, cell lines derived from tumors obtained by injecting the original immortalized human bronchial epithelial cell lines into nude mice exhibited enhanced invasive and metastatic abilities and increased level of type IV collagenase mRNA when compared with the original immortalized human bronchial epithelial cell lines. Invasiveness and metastatic capacity correlated positively with expression of the type IV collagenase gene and negatively with the expression of the type IV procollagen gene, suggesting that these phenotypes are associated both with decreased production and increased dissolution of extracellular matrix.
authors.affiliation: Department of Pathology, Fox Chase Cancer Centre, Philadelphia, Pennsylvania 19111.
authors.forename: H
authors.initials: H
authors.lastname: Ura
authors.forename: R D
authors.initials: RD
authors.lastname: Bonfil
authors.forename: R
authors.initials: R
authors.lastname: Reich
authors.forename: R
authors.initials: R
authors.lastname: Reddel
authors.forename: A
authors.initials: A
authors.lastname: Pfeifer
authors.forename: C C
authors.initials: CC
authors.lastname: Harris
authors.forename: A J
authors.initials: AJ
authors.lastname: Klein-Szanto
mesh_terms: D000818:Animals; D001980:Bronchi; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D002633:Chemotaxis; D004847:Epithelial Cells; D005796:Genes; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology* / Q000503:physiopathology; D051379:Mice; D003012:Microbial Collagenase / Q000235:genetics*; D009362:Neoplasm Metastasis; D009857:Oncogenes; D011347:Procollagen / Q000235:genetics*; D013997:Time Factors; D014158:Transcription, Genetic
chemical_list: D011347:Procollagen; D003012:Microbial Collagenase