title: Cytoskeletal reorganization during electric-field-induced fusion of Chinese hamster ovary cells grown in monolayers.
abstract: Mammalian cells were shown to fuse after direct electric pulsation of the plated cells in culture. The extent of fusion was controlled by the duration of the post-pulse incubation. Formation of polynucleated cells was slow, even at 37 degrees C. Pre-pulse incubation with colchicine increased the fusion yield slightly. Cytoskeletal organization during the post-pulse incubation was observed using immunofluorescence techniques. Microfilaments were unaffected, but microtubules disappeared during the first minutes following the pulse, and then reformed on subsequent incubation.
authors.affiliation: Centre de Biochimie et de Génétique cellulaires du CNRS, Toulouse, France.
authors.forename: C
authors.initials: C
authors.lastname: Blangero
authors.forename: M P
authors.initials: MP
authors.lastname: Rols
authors.forename: J
authors.initials: J
authors.lastname: Teissié
mesh_terms: D008841:Actin Cytoskeleton / Q000648:ultrastructure; D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D002459:Cell Fusion; D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure; D003078:Colchicine / Q000494:pharmacology; D006224:Cricetinae; D003412:Cricetulus; D003571:Cytochalasin B / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects / Q000648:ultrastructure*; D004560:Electricity; D005260:Female; D005455:Fluorescent Antibody Technique; D008870:Microtubules / Q000648:ultrastructure; D010053:Ovary; D017239:Paclitaxel; D013696:Temperature; D013997:Time Factors
chemical_list: D000470:Alkaloids; D003571:Cytochalasin B; D017239:Paclitaxel; D003078:Colchicine