Expression of foreign DNA in Chlamydomonas reinhardtii.
A chimeric octopine synthase-neomycin phosphotransferase (ocs-nptII) gene was used to transform Chlamydomonas reinhardiii to kanamycin resistance. Southern hybridization using DNA isolated from one transformant, T6.1, indicated that the entire ocs-nptII gene and at least part of the plasmid were integrated into nuclear DNA. Neomycin phosphotransferase II activity has been detected in T6.1 cell extracts. Northern hybridizations, employing a radiolabeled ocs-nptII sequence, revealed a T6.1 transcript of approximately the same size as a homologous transcript isolated from E. coli carrying the nptII gene. Although T6.1 is an extremely rare example of a stable C. reinhardtii transformant, its occurrence nevertheless indicates that bacterial genes can be expressed in the nucleus of the alga.
Bingham
S E
SE
Martek Corporation, Columbia, MD 21045.
Cox
J C
JC
Strem
M D
MD
D000594:Amino Acid Oxidoreductases / Q000235:genetics*; D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D002678:Chimera; D002696:Chlamydomonas / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D004247:DNA / Q000096:biosynthesis; D015870:Gene Expression; D019868:Kanamycin Kinase; D007613:Kanamycin Resistance; D000587:Oxidoreductases Acting on CH-NH Group Donors; D010641:Phenotype; D010770:Phosphotransferases / Q000096:biosynthesis / Q000235:genetics*; D014170:Transformation, Genetic
D004247:DNA; D000594:Amino Acid Oxidoreductases; D000587:Oxidoreductases Acting on CH-NH Group Donors; C021903:D-octopine dehydrogenase; D010770:Phosphotransferases; D019868:Kanamycin Kinase