title: Increased contact hypersensitivity response in mice by topical application of 1 alpha,25-dihydroxyvitamin D3 to elicitation site.
abstract: Recent evidence indicates that the biologically active metabolite of vitamin D3, 1 alpha,25-dihydroxyvitamin D3 [1 alpha,25(OH)2D3], has an effect on the regulation of the immune response. We investigated whether topical treatment of mice with 1 alpha,25(OH)2D3 influences the contact hypersensitivity (CHS) response to trinitrochlorobenzene (TNCB). 1 alpha,25(OH)2D3 was applied to the dorsal trunk of A/J mice on days 0-3, and on day 4 topical application of 5% TNCB on the 1 alpha,25(OH)2D3-treated site was performed. The mice were tested for CHS on day 10 by applying 1% TNCB to the ears. No effect on induction of CHS response to TNCB was observed in 1 alpha,25(OH)2D3-treated mice compared with 24,25-dihydroxyvitamin D3[24,25(OH)2D3]-treated mice as control. In a second experiment, the dorsal trunk of A/J mice was treated with 5% TNCB on day 0. The topical application of 1 alpha,25(OH)2D3 on the ears was performed from days 2 to 5. On day 6, the mice were tested for CHS by applying 1% TNCB to the 1 alpha,25(OH)2D3-treated ears. When 1 alpha,25(OH)2D3 was administered to the elicitation site prior to the challenge, pretreatment of mice with 1 alpha,25(OH)2D3 increased their response to TNCB by 40% compared with 24,25(OH)2D3-treated mice as control (P less than 0.01). There were no findings suggesting that the pretreatment of the challenge site with 1 alpha,25(OH)2D3 induced an irritant dermatitis that was superimposed on a subsequent CHS reaction. The 1 alpha,25(OH)2D3 modulation of CHS response to TNCB in mice suggests that the hormone may play a role in the regulation of the immune response in vivo.
authors.affiliation: Department of Dermatology, Kobe University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Tani
authors.forename: Y
authors.initials: Y
authors.lastname: Murata
authors.forename: S
authors.initials: S
authors.lastname: Harada
authors.forename: T
authors.initials: T
authors.lastname: Takashima
authors.forename: T
authors.initials: T
authors.lastname: Horikawa
mesh_terms: D000287:Administration, Topical; D000818:Animals; D000954:Antigens, Surface; D002117:Calcitriol / Q000008:administration & dosage / Q000494:pharmacology; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology; D003877:Dermatitis, Contact / Q000276:immunology*; D000078404:Epidermal Cells; D005260:Female; D007801:Langerhans Cells / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D010853:Picryl Chloride / Q000276:immunology; D018800:Thy-1 Antigens
chemical_list: D000954:Antigens, Surface; D018800:Thy-1 Antigens; D002117:Calcitriol; D010853:Picryl Chloride