The differential overamplification of short sequences in the mitochondrial DNA of rho- petites in Saccharomyces cerevisiae stimulates recombination.
It is well known that the yeast Saccharomyces cerevisiae can be affected by mutations, called 'petite colonie', which correspond to the loss of the major part of the mitochondrial DNA and the concomitant amplification of the remaining sequence, the basic repeat unit (BRU). We describe here a new phenomenon, the internal overamplification (IOA), due to the differential amplification (up to 20-fold) of short sequences within the BRU. These IOAs are very stable and stimulate the recombination. We discuss here the possible mechanisms giving rise to the appearance and maintenance of the IOAs within the BRU and their effect on the recombination process.
Netter
P
P
Laboratoire Propre Associé à l'Université Pierre et Marie Curie, Centre de Génétique Moléculaire du C.N.R.S., Gif-sur-Yvette, France.
Robineau
S
S
D001483:Base Sequence; D004262:DNA Restriction Enzymes / Q000032:analysis; D004272:DNA, Mitochondrial; D003720:Densitometry; D005784:Gene Amplification; D008969:Molecular Sequence Data; D009154:Mutation; D011995:Recombination, Genetic / Q000502:physiology*; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D004272:DNA, Mitochondrial; D004262:DNA Restriction Enzymes