Simultaneous determination of iodate and periodate by capillary zone electrophoresis: application to carbohydrate analysis.
Iodate and periodate were rapidly (in 11 min) separated from each other with high column efficiency by capillary zone electrophoresis, using a fused silica tube (50 microns i.d., 80 cm) and 100 mM acetate buffer, pH 4.5, as carrier. On-column uv detection at 222 nm allowed sensitive detection down to the picomole level, and measurement of relative peak area to that of pyromellitic acid (internal standard) enabled reproducible determination of these ions. This method was proved useful for periodate oxidation analysis of various carbohydrates.
Honda
S
S
Faculty of Pharmaceutical Sciences, Kinki University, Higashi-osaka, Japan.
Suzuki
K
K
Kakehi
K
K
D002241:Carbohydrates / Q000032:analysis*; D004586:Electrophoresis / Q000379:methods; D006863:Hydrogen-Ion Concentration; D007452:Iodates / Q000032:analysis*; D007455:Iodine / Q000032:analysis*; D010084:Oxidation-Reduction; D010504:Periodic Acid / Q000032:analysis*; D012492:Salts
D002241:Carbohydrates; D007452:Iodates; D012492:Salts; D010504:Periodic Acid; D007455:Iodine