[Genetic instability of determinants of resistance to chloramphenicol and kanamycin in Streptomyces lividans 66].
The frequency of chloramphenicol-sensitive variants (Cmls) in Streptomyces lividans 66 is very high (0.57%). Correlation between chloramphenicol sensitivity and deamplification of PstI fragment with the length of 4.82 kb (RES1 genetic element) was shown. However, in some Cmls variants there was no RES1 deamplification. It was noted that in the cells of the Cmls variants isolated the levels of kanamycin and neomycin resistance determined by the Kanr determinant in the pSU17 plasmid were different. Expression of Kanr and Neor determinants inserted via pSU17 plasmid into the cells of Cmls variants was studied and three classes of chloramphenicol-sensitive variants were defined. After transformation of pSU17 plasmid into cells of Cmls variants of the class I, expression of Kanr and Neor genes, similar to that in S. lividans 66, was observed. The resistance level in Cmls variants of the class II was intermediate. In the cells of the class III no expression was noted. Cmls strains of classes I and II were unstable and those of the class III with impaired expression of Kanr and Neor genes were formed with high frequency. Cmlr variants formed from Cmls strain of the class III were studied. Two types of Cmlr variants were detected. Variants of the first type were identical to S. lividans 66 by their properties. The frequency of Cmls variants occurring in the cells of the first type was similar to that in S. lividans 66. The second type included pseudo-revertants. They were unstable and generated amplifications of the 5.7 kb fragment with high frequency.
Starodubtseva
L I
LI
Taisova
A S
AS
Danilenko
V I
VI
D002702:Chloramphenicol Resistance / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005784:Gene Amplification; D015964:Gene Expression Regulation, Bacterial; D019868:Kanamycin Kinase; D007613:Kanamycin Resistance / Q000235:genetics*; D010770:Phosphotransferases / Q000378:metabolism; D010957:Plasmids; D015183:Restriction Mapping; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics*; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D010770:Phosphotransferases; D019868:Kanamycin Kinase
Geneticheskaia nestabilnost' determinantov ustoĭchivosti k khloramfenikolu i kanamitsinu v shtamme Streptomyces lividans 66.