A novel CD3-J11d+ subset of CD4+CD8- cells repopulating thymus in radiation bone marrow chimeras.
Sequential appearance of T cell subpopulations occurs in the thymus of irradiated AKR (H-2k, Thy-1.1) mice at an early stage after transplantation with bone marrow cells of C3H/HeN (H-2k, Thy-1.2) mice. The donor-derived thymocytes were first detected on day 8 after bone marrow reconstitution. Although most of the thymocytes were CD4-CD8- cells, an appreciable level of CD4+CD8- cells was detected in the thymus at this stage. The early appearing CD4+CD8- cells were a novel subset of thymocytes that were J11d+CD3-. From day 10 to day 21 the proportion of CD4+CD8-CD3-J11d+ cells decreased while the proportion of CD4+CD8+ cells and CD4+CD8-CD3+J11d- cells increased. The CD4+CD8-CD3- cells seem to diversify to form CD4+CD8+ thymocytes after short-term culture in vitro. These results suggested the existence of a differential pathway from CD4-CD8- cells to CD4+CD8+ cells via CD4+CD8- cells in thymus.
Matsumoto
K
K
Department of Immunology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.
Yoshikai
Y
Y
Matsuzaki
G
G
Asano
T
T
Nomoto
K
K
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D016026:Bone Marrow Transplantation; D017252:CD3 Complex; D015496:CD4-Positive T-Lymphocytes / Q000145:classification* / Q000276:immunology; D016827:CD8 Antigens; D002454:Cell Differentiation; D005434:Flow Cytometry; D051379:Mice; D008815:Mice, Inbred Strains; D011828:Radiation Chimera; D011948:Receptors, Antigen, T-Cell / Q000032:analysis; D013950:Thymus Gland / Q000166:cytology*; D013997:Time Factors
D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D016827:CD8 Antigens; D011948:Receptors, Antigen, T-Cell