title: Distribution of M protein and nucleocapsid protein of vesicular stomatitis virus in infected cell plasma membranes.
abstract: The association of M protein and the nucleocapsid (N) protein of vesicular stomatitis virus (VSV) with the cytoplasmic surface of plasma membranes prepared from infected cells was examined by double label immunofluorescence. M protein in association with the cytoplasmic surface of the plasma membrane was distributed in two distinct labeling patterns. Punctate labeling of M protein in the plasma membrane was observed in association with corresponding labeling for the nucleocapsid protein. Diffusely labeled M protein was distributed in areas of the plasma membrane that were devoid of any detectable labeling for the nucleocapsid protein. Similar results were obtained with two different cell types at 4 h and later times postinfection. The diffuse label for M protein was present in membranes prepared from cells infected with a temperature-sensitive M protein mutant at the nonpermissive temperature, but neither the punctate label for M protein nor labeling for the nucleocapsid protein was observed. Upon shift to permissive temperature, both the punctate label for M protein and labeling for the nucleocapsid protein began to reappear in membranes prepared from cells infected with the M protein mutant. These results indicate that M protein can associate with the plasma membrane without prior binding to nucleocapsids and that association of functional M protein with the plasma membrane is required for the stable association of nucleocapsids with the membrane during the process of viral budding.
authors.affiliation: Department of Microbiology and Immunology, Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, NC 27103.
authors.forename: B J
authors.initials: BJ
authors.lastname: McCreedy
authors.forename: D S
authors.initials: DS
authors.lastname: Lyles
mesh_terms: D000818:Animals; D001665:Binding Sites; D002213:Capsid / Q000378:metabolism; D002461:Cell Line, Transformed; D002462:Cell Membrane / Q000378:metabolism; D009154:Mutation; D013696:Temperature; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics / Q000378:metabolism*; D014758:Viral Core Proteins / Q000378:metabolism; D014763:Viral Matrix Proteins / Q000378:metabolism; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D014758:Viral Core Proteins; D014763:Viral Matrix Proteins; D014764:Viral Proteins