title: Performing nucleic acid reactions using predispensed lyophilized reaction mixtures.
abstract: A system is described in which manipulations of nucleic acids are performed in wells containing predispensed lyophilized reaction mixtures requiring addition of only nucleic acid. This allows increased reproducibility for single-step reactions (e.g., restrictions and ligations), as well as improved productivity for complex reactions (e.g., sequencing). Enzymes, co-factors, nucleotides and buffers can be dried and stored at room temperature without loss of essential function. When used for DNA sequencing, hundreds of templates a day can be sequenced with the potential to determine megabase amounts of sequence per week.
authors.affiliation: Surgical Unit, London Hospital Medical College, University of London, Whitechapel, U.K.
authors.forename: S A
authors.initials: SA
authors.lastname: Ortlepp
authors.forename: I A
authors.initials: IA
authors.lastname: McKay
mesh_terms: D001483:Base Sequence; D001709:Biotechnology; D004247:DNA; D011088:DNA Ligases; D004262:DNA Restriction Enzymes; D005612:Freeze Drying; D007202:Indicators and Reagents; D009696:Nucleic Acids
chemical_list: D007202:Indicators and Reagents; D009696:Nucleic Acids; D004247:DNA; D004262:DNA Restriction Enzymes; D011088:DNA Ligases