A continuous line of chicken embryo cells derived from a chondrocyte culture infected with RSV.
A continuous line of chicken embryo cells was derived from a culture of chondrocytes infected with Rous sarcoma virus (RSV). These cells, designated as NA101, have reduced serum requirements and are able to grow in a semisolid medium. NA101 cells show the same phenotype as freshly RSV infected chondrocyte cultures, i.e. synthesis of fibronectin and type I collagen, elongated bipolar shape and absence of tumorigenicity following grafting onto the chorioallantoic membrane of embryonated duck eggs; thus they appear to be distinct from transformed fibroblasts. Neither NA101 cells nor freshly infected chondrocyte cultures synthesize type II or type X collagen, which are the differentiation markers of normal chicken chondrocytes in culture.
Gionti
E
E
Department of Biochemistry and Medical Biotechnology, 2nd Medical School, University of Naples, Italy.
Jullien
P
P
Pontarelli
G
G
Sanchez
M
M
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000502:physiology*; D002356:Cartilage / Q000166:cytology*; D002455:Cell Division; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic; D002642:Chick Embryo; D004591:Electrophoresis, Polyacrylamide Gel; D007621:Karyotyping; D008856:Microscopy, Fluorescence; D015688:Oncogene Protein pp60(v-src) / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism
D011494:Protein Kinases; D015688:Oncogene Protein pp60(v-src)