title: The insulin-like effects of phorbol myristate acetate (PMA) in the isolated fat cell.
abstract: Recent data from many laboratories suggest that insulin stimulates diacylglycerol formation. Data presented in this manuscript demonstrate an insulin-like effect of PMA, a tumor promoting agent that mimics the action of diacylglycerol, in isolated adipocytes on; (a) glucose oxidation using uniformly labelled, C-1-labelled and C-6-labelled glucose, (b) epinephrine-induced lipolysis and (c) low Km cAMP phosphodiesterase activity. Additionally, a lipolytic effect of PMA is identified when unopposed by epinephrine. These data not only demonstrate an insulin-like effect of phorbol esters in adipose tissue but they lend support to the concept of diacylglycerol involvement in the mechanism of insulin action.
authors.affiliation: Department of Research, VA Hospital, Memphis, Tennessee.
authors.forename: S S
authors.initials: SS
authors.lastname: Solomon
authors.forename: M
authors.initials: M
authors.lastname: Palazzolo
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D004837:Epinephrine / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis / Q000187:drug effects; D007328:Insulin / Q000494:pharmacology*; D007700:Kinetics; D008066:Lipolysis / Q000187:drug effects*; D008297:Male; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D011865:Radioisotope Dilution Technique; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
chemical_list: D002250:Carbon Radioisotopes; D007328:Insulin; D010727:Phosphoric Diester Hydrolases; D005947:Glucose; D013755:Tetradecanoylphorbol Acetate; D004837:Epinephrine