A voltage-dependent calcium current in mouse MC3T3-E1 osteogenic cells.
MC3T3-E1 osteogenic cells in a growing state were voltage-clamped by the whole-cell patch-clamp method. The MC3T3-E1 cells exhibited a transient, fast-inactivating Ca inward current upon depolarizing pulses from a holding potential of -80 mV. This current had a threshold of activation of about -50 mV and was insensitive to the dihydropyridine, nifedipine. These results show that MC3T3-E1 cells have a voltage-dependent Ca channel corresponding to the "T-type."
Amagai
Y
Y
Department of Physiology, Ohu University School of Dentistry, Koriyama, Japan.
Kasai
S
S
D000818:Animals; D015220:Calcium Channels / Q000502:physiology*; D002460:Cell Line; D002999:Clone Cells; D004594:Electrophysiology; D051379:Mice; D009543:Nifedipine / Q000494:pharmacology; D010006:Osteoblasts / Q000187:drug effects / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology*
D015220:Calcium Channels; D009543:Nifedipine