title: Restriction fragment length polymorphisms associated with growth hormone and prolactin genes in Holstein bulls: evidence for a novel growth hormone allele.
abstract: Sperm DNA isolated from sons of three extensively used US Holstein bulls was screened for differences associated with the primary gene structure of the bovine growth hormone (bGH) and prolactin (bPrl) genes. Southern blot analysis of DNA digested with 10 restriction enzymes revealed that offspring from two of the three bull families exhibited polymorphisms around the bGH and bPrl genes. Restriction fragment length polymorphisms (RFLPs) around the bGH gene were detected with five enzymes, whereas three enzymes revealed RFLPs around the bPrl gene. At least three structural differences were predicted around the bGH gene. The most common variant hybridization pattern appeared to involve an insertion/deletion located downstream of the conserved 3' EcoRI site. The presence of RFLPs in the genes coding for these pituitary hormones within a familial line may provide the basis for genetic markers associated with lactation and mammary development.
authors.affiliation: Department of Dairy Science, University of Wisconsin, Madison 53706.
authors.forename: C M
authors.initials: CM
authors.lastname: Cowan
authors.forename: M R
authors.initials: MR
authors.lastname: Dentine
authors.forename: R L
authors.initials: RL
authors.lastname: Ax
authors.forename: L A
authors.initials: LA
authors.lastname: Schuler
mesh_terms: D000483:Alleles; D000818:Animals; D015139:Blotting, Southern; D002417:Cattle / Q000235:genetics*; D002872:Chromosome Deletion; D004247:DNA / Q000235:genetics; D013006:Growth Hormone / Q000235:genetics*; D008297:Male; D009693:Nucleic Acid Hybridization; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011388:Prolactin / Q000235:genetics*
chemical_list: D011388:Prolactin; D013006:Growth Hormone; D004247:DNA