Immortalized phenotype and the presence of active oncogenes correlate with the capacity of culture cells to induce reactivation of DNA synthesis in macrophage nuclei in heterokaryons.
Several types of culture cells with limited life span (rat embryo fibroblasts, rat chondrocytes and mouse premacrophages) were found to be unable to induce the reactivation of DNA synthesis in the nuclei of non-dividing differentiated cells (mouse peritoneal resident macrophages) in heterokaryons. By contrast, malignant HeLa cells have this ability. In heterokaryons formed by fusion of mouse macrophages with HE239 cells (Syrian hamster fibroblasts transformed with a ts mutant of the SV40 virus), DNA synthesis in macrophage nuclei is reactivated only at the permissive temperature (33 degrees C), at which viral T antigen is stable. Immortalization of rat chondrocytes by transfection with p53 gene enables to induce DNA synthesis in macrophage nuclei upon fusion. All the evidence indicates that the function of immortalizing oncogenes is necessary for the resumption of the DNA synthesis in macrophage nuclei in heterokaryons.
Prudovsky
I A
IA
Engelhardt Institute of Molecular Biology of the U.S.S.R. Academy of Sciences, Moscow.
Gumeniuk
R R
RR
Yegorov YeE
Poletayev
A I
AI
Chumakov
P M
PM
Zelenin
A V
AV
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000378:metabolism; D002356:Cartilage / Q000166:cytology / Q000378:metabolism; D002459:Cell Fusion; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D002478:Cells, Cultured; D006224:Cricetinae / Q000235:genetics*; D004247:DNA / Q000096:biosynthesis*; D005347:Fibroblasts / Q000378:metabolism; D005786:Gene Expression Regulation; D006367:HeLa Cells; D008264:Macrophages / Q000166:cytology / Q000378:metabolism*; D008647:Mesocricetus / Q000235:genetics*; D051379:Mice; D008808:Mice, Inbred CBA / Q000235:genetics*; D009857:Oncogenes; D010641:Phenotype; D010957:Plasmids; D051381:Rats; D013539:Simian virus 40 / Q000235:genetics; D013696:Temperature; D014162:Transfection; D014170:Transformation, Genetic
D000952:Antigens, Polyomavirus Transforming; D004247:DNA