Reactivation of antibody genes in Epstein-Barr virus transformed human B cell lines. A preliminary report.
Three immortalised human B cell lines, which had either last their capacity to secrete specific antibody or secreted low levels of antibody were studied in an attempt to reactivate or enhance antibody synthesis. A variety of different stimuli known to cause activation, proliferation and differentiation of normal B cells were used including polyclonal activators and recombinant interferon. Four of them, (namely LPS, anti-IgM, IL2 and IL6) effectively increased specific antibody synthesis after three days of culture. These preliminary experiments show that the loss or decline in immunoglobulin production by immortalised human B cells is, at least in some cases, reversible.
Skibiński
G
G
Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw.
James
K
K
D000917:Antibody Formation / Q000187:drug effects / Q000235:genetics*; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D002460:Cell Line; D004854:Herpesvirus 4, Human; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology*; D007378:Interleukins / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology*
D007106:Immune Sera; D007378:Interleukins; D008070:Lipopolysaccharides