Mannose-resistant hemagglutination, enzyme-linked immunosorbent assay, and immune electron microscopy for detection of K99 fimbrial antigen in Escherichia coli from calves.
Mannose-resistant hemagglutination (MRHA) was evaluated for identification of Escherichia coli with K99 fimbriae. The sensitivity and specificity of MRHA, relative to the enzyme-linked immunosorbent assay, were 21 and 79%, respectively. Disagreement between the tests may have been due in part to separation of pili from the cells, with resultant enzyme-linked immunosorbent assay-positive, MRHA-negative tests. MRHA was not useful as the sole means for the identification of E. coli with K99 fimbriae.
Hernández
F
F
Centro de Investigación y Diagnostico en Parasitologia, Facultad de Microbiologia, Universidad de Costa Rica.
Caballero
M
M
Rivera
P
P
Hird
D
D
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis*; D001427:Bacterial Toxins; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000276:immunology* / Q000648:ultrastructure; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D005260:Female; D010861:Fimbriae, Bacterial / Q000276:immunology* / Q000648:ultrastructure; D006386:Hemagglutination Tests; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001427:Bacterial Toxins; C031429:K99 antigen; D011933:Reagent Kits, Diagnostic