Identification and production of monoclonal antibodies against a discriminating protein molecule between B and D variants of encephalomyocarditis virus.
The protein profiles of two EMC virus variants B and D in the infected L929 fibroblast lysates were examined using one- and two-dimensional gel electrophoresis. Both variants yielded a protein molecule of similar molecular weight but differing in its isoelectric point (pI). The B variant lysate yielded a molecule with pI congruent to 7.5 whereas the same molecule from the D lysate focused at pI congruent to 5.2. A monoclonal antibody (MCA) produced against pI congruent to 5.2 fraction of the D variant successfully detected viral antigens in the D variant infected fibroblasts with only background cross reactivity with the B variant infected cells. This MCA also detected D viral antigen(s) in organ sections obtained from D but not from B variant infected mice. This study therefore suggests a clear shift in the pI of a 23 kD protein molecule serving as a possible discriminating antigen between the B and D variants of EMC virus.
Qureshi
M A
MA
Molecular Genetics Laboratory, Guthrie Research Institute, Sayre, PA 18840-1692.
Yoon
J W
JW
Kwon
B S
BS
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000302:isolation & purification; D003920:Diabetes Mellitus / Q000382:microbiology; D004686:Encephalomyocarditis virus / Q000235:genetics / Q000276:immunology*; D014644:Genetic Variation; D006801:Humans; D007515:Islets of Langerhans / Q000382:microbiology; D007526:Isoelectric Point; D051379:Mice; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D014764:Viral Proteins