[An analysis of the action of nonspecific and restriction nucleases on metaphase chromosomes in situ].
Patterns of differential staining of Drosophila, mouse, rat, cattle and pig chromosomes were examined after the treatment with nucleases (DNAase I, DNAase II) and restriction enzymes (AluI, HpaII, MspI, BpE, EcoRI). The above effects depend on the species used, on the enzymes and substitution of thymine for bromodeoxyuridine in the chromosomal DNA. It is supposed that such a phenomenon may not only result from the irregular distribution of specific restriction sites along chromosomes but also depend on the specificity of supramolecular organization of the chromosomal DNA.
Smirnov
A F
AF
Patkin
E L
EL
Stefanova
V N
VN
Chiriaeva
O G
OG
Sorokin
A V
AV
D000818:Animals; D002417:Cattle; D002871:Chromosome Banding; D002875:Chromosomes / Q000187:drug effects* / Q000648:ultrastructure; D004262:DNA Restriction Enzymes / Q000494:pharmacology*; D003851:Deoxyribonucleases / Q000494:pharmacology*; D004331:Drosophila melanogaster; D008677:Metaphase / Q000187:drug effects*; D051379:Mice; D008938:Mitosis / Q000187:drug effects; D051381:Rats; D013045:Species Specificity; D013552:Swine; D013997:Time Factors
D003851:Deoxyribonucleases; D004262:DNA Restriction Enzymes
Analiz deĭstviia nespetsificheskikh i restriktsionnykh nukleaz na metafaznye khromosomy in situ.