Influence of transglutaminase on the functions of mouse peritoneal macrophages.
Influence of transglutaminase on the production of interleukin-1 (IL-1) and on the release of active oxygen from mouse peritoneal macrophages was examined using cystamine and methylamine, an enzyme inhibitor and a substrate inhibitor, respectively. Casein-elicited or lipopolysaccharide (LPS)-elicited macrophages have higher levels of transglutaminase activity in comparison with resident macrophages, and there exists a definite correlation between endocytosis of erythrocytes and transglutaminase activity in either group of macrophages. The release of IL-1 by resident macrophages stimulated with LPS in vitro was significantly inhibited by the treatment with both transglutaminase inhibitors. However, these inhibitors were not able to inhibit the release of IL-1 from casein-elicited macrophages stimulated with LPS in vitro. The production of active oxygen from LPS-elicited macrophages was inhibited in a dose-dependent manner by the treatment of macrophages with cystamine, but was not by the treatment with methylamine. However, the treatment of LPS-elicited macrophages with cystamine did not inhibit the uptake of glucose into macrophages. These results suggest that transglutaminase activity in mouse peritoneal macrophages is an important factor for macrophage functions.
Ishitani
K
K
Department of Microbiology, Tohoku College of Pharmacy, Miyagi.
Suzuki
M
M
D000818:Animals; D003538:Cystamine / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism; D007375:Interleukin-1 / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008163:Luminescent Measurements; D008264:Macrophages / Q000201:enzymology* / Q000378:metabolism / Q000502:physiology; D008297:Male; D008744:Methylamines / Q000494:pharmacology; D051379:Mice; D008809:Mice, Inbred C3H; D010100:Oxygen / Q000378:metabolism; D010529:Peritoneal Cavity / Q000166:cytology; D010587:Phagocytosis; D011503:Transglutaminases / Q000037:antagonists & inhibitors / Q000378:metabolism*
D007375:Interleukin-1; D008070:Lipopolysaccharides; D008744:Methylamines; C027451:methylamine; D011503:Transglutaminases; D005947:Glucose; D003538:Cystamine; D010100:Oxygen