Gas chromatographic determination of nicotine in environmental tobacco smoke: collaborative study.
A gas chromatographic method for determination of vapor phase nicotine in environmental tobacco smoke (ETS) was collaboratively studied by 6 laboratories. Nicotine is desorbed from XAD-4 sample tubes with ethyl acetate containing triethylamine and determined by gas chromatography with nitrogen-selective detection. Each collaborator received blind duplicate samples at each of 6 nicotine concentrations. Three concentrations were generated by spiking XAD-4 tubes with known amounts of nicotine; the remaining 3 concentrations were ETS samples obtained in a carefully controlled environmental chamber containing sidestream and exhaled mainstream smoke from 1R4F Kentucky reference cigarettes. Repeatability and reproducibility relative standard deviations ranged from 4.4 to 11.1% and from 7.0 to 11.1%, respectively, for nicotine concentrations evaluated (up to 6 micrograms/cu m). The method has been adopted official first action.
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R.J. Reynolds Tobacco Co., Winston-Salem, NC 27102.
D002849:Chromatography, Gas; D007202:Indicators and Reagents; D009538:Nicotine / Q000032:analysis*; D014028:Tobacco Smoke Pollution / Q000032:analysis*
D007202:Indicators and Reagents; D014028:Tobacco Smoke Pollution; D009538:Nicotine