Isolation and plaque purification of a syncytial forming component of myxoma virus.
Using the endpoint dilution and plaque purification technique performed on rabbit kidney cells, two stable syncytial clones of myxoma virus have been isolated. No eosinophilic intracytoplasmic inclusion bodies nor intranuclear granulations could be detected in cells infected with these clones. Traditional plaques with the central area of detached cells surrounded by round cells also could not be recognized. However, when enough virus was added, one large complete syncytium developed in 24 on the monolayer. Syncytial activity was associated with the entire particle. This was the first time that myxoma virus is reported to demonstrate such an intense syncytial activity.
Bijlenga
G
G
Chaire de Maladies Contagieuses, Ecole Nationale Vétérinaire de Lyon, Charbonnières, France.
Duclos
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P
D000818:Animals; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D008854:Microscopy, Electron; D009233:Myxoma virus / Q000302:isolation & purification* / Q000502:physiology / Q000648:ultrastructure; D014771:Virion / Q000302:isolation & purification* / Q000502:physiology / Q000648:ultrastructure