A new DNA marker (D4S90) is located terminally on the short arm of chromosome 4, close to the Huntington disease gene.
Genetic linkage studies have mapped Huntington's disease (HD) to the distal portion of the short arm of chromosome 4 (4p16.3), 4 cM distal to D4S10 (G8). To date, no definite flanking marker has been identified. A new DNA marker, D4S90 (D5); which maps to the distal region of 4p16.3, is described. The marker was used in a genetic linkage study in the CEPH reference families with seven other markers at 4p16. The study, together with knowledge of the physical map of the region, places D4S90 as the most distal marker, 6 cM from D4S10. A provisional linkage study with HD gave a maximum lod score of 2.14 at a theta of 0.00 and no evidence of linkage disequilibrium. As D4S90 appears to be located terminally, it should play an important role in the accurate mapping and cloning of the HD gene.
Youngman
S
S
Institute of Medical Genetics, University of Wales College of Medicine, Heath Park, Cardiff, United Kingdom.
Sarfarazi
M
M
Bucan
M
M
MacDonald
M
M
Smith
B
B
Zimmer
M
M
Gilliam
C
C
Frischauf
A M
AM
Wasmuth
J J
JJ
Gusella
J F
JF
D015139:Blotting, Southern; D002874:Chromosome Mapping; D002894:Chromosomes, Human, Pair 4; D004247:DNA / Q000235:genetics; D005260:Female; D008040:Genetic Linkage; D005819:Genetic Markers / Q000032:analysis; D005838:Genotype; D006801:Humans; D006816:Huntington Disease / Q000235:genetics*; D008297:Male; D010375:Pedigree; D012150:Polymorphism, Restriction Fragment Length; D011995:Recombination, Genetic
D005819:Genetic Markers; D004247:DNA