Cloning and characterization of the yeast chaperonin HSP60 gene.
The heat-shock protein, HSP60, is abundant in prokaryotes and eukaryotes and is required in the assembly of specific proteins. We have cloned the Saccharomyces cerevisiae HSP60 gene from a lambda gt11 genomic library using monoclonal antibodies, have obtained its sequence, determined its transcription start point, and shown that it exists as a single copy. The predicted HSP60 contains a mitochondrial target sequence and exhibits striking amino acid sequence similarity to its counterparts in bacteria, plants, and humans. These data indicate a high level of evolutionary conservation and are consistent with the suggestion of evolutionarily conserved function [Hemmingsen et al., Nature 333 (1988), 330-334].
Johnson
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Whitehead Institute for Biomedical Research, Cambridge, MA.
Fearon
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Mason
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Jindal
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D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D018833:Chaperonins; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D005656:Fungal Proteins / Q000235:genetics*; D015723:Gene Library; D005798:Genes, Bacterial; D005800:Genes, Fungal; D006360:Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D010944:Plants / Q000235:genetics; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D015719:Single-Strand Specific DNA and RNA Endonucleases
D000911:Antibodies, Monoclonal; D004271:DNA, Fungal; D005656:Fungal Proteins; D006360:Heat-Shock Proteins; D011506:Proteins; D015719:Single-Strand Specific DNA and RNA Endonucleases; D018833:Chaperonins