Flow cytometric analysis of protein kinase C in lymph node cells during the induction phase of contact hypersensitivity reaction.
On the fourth day after a single painting of 2,4,6-trinitro-chlorobenzene on the abdominal skin, inguinal lymph nodes were removed, and a single-cell suspension was prepared. The cells were analyzed flow cytometrically, using monoclonal anti-protein kinase C antibody. It was found that the number of lymph node cells in which protein kinase C was detected on the cell surface was significantly increased over that in non-treated mice (p less than 0.01). On the basis of our results and discussions in the literature, it is thought that protein kinase C is related to the initiation of the contact hypersensitivity reaction.
Morita
H
H
Yamagata
M
M
Inohara
S
S
Sagami
S
S
D000818:Animals; D003877:Dermatitis, Contact / Q000201:enzymology* / Q000209:etiology; D005434:Flow Cytometry; D008198:Lymph Nodes / Q000201:enzymology; D051379:Mice; D008809:Mice, Inbred C3H; D010853:Picryl Chloride / Q000633:toxicity; D011493:Protein Kinase C / Q000378:metabolism*
D011493:Protein Kinase C; D010853:Picryl Chloride