[Establishment of a human skin squamous cell carcinoma cell line (SCC-1CB) in vitro and its characteristics].
A human skin tumor cell line SCC-1CB has been established in vitro. A specimen from a surgically excised tumor with moderately differentiated squamous cell carcinoma of skin from a Japanese male, aged 62, was transplanted subcutaneously into nude mice. After growth, a part of the tumor was passaged with a transplantation needle. At the fourth passage, part of the tumor was explanted into culture dishes. Cell cultures were maintained in Eagle's minimal essential medium supplemented with 10% fetal calf serum. A SCC cell line (SCC-1CB) was cloned and then established. Chromosome analysis revealed the human type with a mode number of 56. The cell line is tumorigenic in nude mice. Immunohistochemical staining revealed that keratin, cytokeratin and vimentin were positive and desmin was negative. It can be cryopreserved in liquid nitrogen. It has successfully maintained proliferation in continuous cell culture for over 2.5 years. The cells retain their original morphological appearance as determined by light and electron microscopy.
Watanabe
F
F
Kojima
T
T
Morita
E
E
Kobayashi
M
M
Fujita
M
M
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D009368:Neoplasm Transplantation; D012878:Skin Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000473:pathology