Hageman factor dependent kinin generation system in guinea pig skin: extravascular localization of the components, and prolonged vascular reaction in inhibitor-depleted animal of this system.
1. Monospecific antibodies against components of the guinea pig Hageman factor-dependent system, such as Hageman factor, prekallikrein and high-molecular-weight (HMW) kininogen were prepared. These antibodies demonstrated the presence of all of these components in the normal guinea pig skin extract with the properties identical to them in plasma on western immunoblotting analysis. 2. Guinea pig macroalbumin which is a major circulating inhibitor of activated Hageman factor was immunologically depleted from three animals. Duration of a vascular permeability reaction caused by an intradermal injection of activated Hageman factor was prolonged in all of these guinea pigs approximately twice as long as the control, indicating a presence of negative feedback regulation of the Hageman factor-dependent kinin generation cascade in dermal tissue via the circulating protease inhibitor.
Yamamoto
T
T
Department of Allergy, Kumamoto University Medical School, Japan.
Ishimatsu
T
T
Kambara
T
T
D000818:Animals; D000906:Antibodies; D002199:Capillary Permeability; D005174:Factor XII / Q000276:immunology / Q000502:physiology*; D005246:Feedback; D005260:Female; D006168:Guinea Pigs; D015151:Immunoblotting; D007705:Kinins / Q000502:physiology*; D008297:Male; D011480:Protease Inhibitors; D012867:Skin / Q000098:blood supply; D012879:Skin Physiological Phenomena
D000906:Antibodies; D007705:Kinins; D011480:Protease Inhibitors; D005174:Factor XII