[Morphologic and enzymatic characteristics of healthy human kidney epithelial tubular cells in culture].
The present study defines morphological and enzymatic characteristics of human healthy renal tubular cells in primary culture, and during three passages. The results confirm the ability to culture human renal tubular cells and to quantitatively evaluate the activity of specific tubular enzymes in the cells and in the culture medium. Such determinations can be compared to similar enzyme activity research in renal biopsies or in urines, to investigate human renal cell functions, in clinical, pharmacological or toxicological approaches.
Merlet
D
D
Laboratoire de Biologie Cellulaire, Faculté de Pharmacie, Bordeaux.
Merlet
J P
JP
Mellado
M
M
Cambar
J
J
D000469:Alkaline Phosphatase / Q000378:metabolism; D002478:Cells, Cultured / Q000201:enzymology; D004847:Epithelial Cells; D004848:Epithelium / Q000201:enzymology; D006801:Humans; D066298:In Vitro Techniques; D007684:Kidney Tubules / Q000166:cytology* / Q000201:enzymology; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D005723:gamma-Glutamyltransferase; D000469:Alkaline Phosphatase
Caractéristiques morphologiques et enzymatiques de cellules épithéliales tubulaires de rein humain sain en culture.