title: The idiotypic characterization of the immune response to a defined epitope of a protein antigen and the specific in vivo suppression of the immune response to this epitope by anti-idiotypic antibodies.
abstract: C10, a monoclonal antibody of C3H.SW (CSW) origin, binds a decapeptide epitope of the tobacco mosaic virus protein (TMVP) representing residues 103-112 of the protein. In vivo administration of syngeneic anti-idiotypic antibodies to C10 (anti-C10) prior to immunization with TMVP suppressed the expression of antibodies to this decapeptide determinant in CSW mice without a significant reduction of the total anti-TMVP titer. The suppression could not be overcome with repeated challenges by antigen even 6 months after administration of anti-C10. Analysis of anti-C10 showed that it contains antibodies to at least two idiotopes found on C10. One of these idiotopes, C10-Idm, is found on a very small fraction of CSW anti-TMVP antibodies capable of binding the decapeptide epitope. The other idiotope, C10-IdX, is found on most of the anti-TMVP antibodies which bind the decapeptide determinant. With synthetic analogues of the decapeptide determinant, a correlation was established between the presence of the C10-IdX and the fine specificity of the decapeptide-binding antibodies. The studies reported herein demonstrate that anti-idiotypic antibodies are potent modulators of the immune response and that the C10-IdX is important in the determination of the fine specificity of antibodies to this decapeptide epitope of TMVP.
authors.forename: F L
authors.initials: FL
authors.lastname: Norton
authors.forename: P R
authors.initials: PR
authors.lastname: Morrow
authors.forename: C Y
authors.initials: CY
authors.lastname: Leung
authors.forename: E
authors.initials: E
authors.lastname: Benjamini
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis* / Q000502:physiology; D000911:Antibodies, Monoclonal / Q000502:physiology*; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D036022:Capsid Proteins; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology*; D007108:Immune Tolerance; D007130:Immunoglobulin Idiotypes / Q000032:analysis* / Q000276:immunology; D008715:Methionine / Q000031:analogs & derivatives; D051379:Mice; D008808:Mice, Inbred CBA; D013045:Species Specificity; D014764:Viral Proteins / Q000008:administration & dosage / Q000276:immunology* / Q000378:metabolism
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D036022:Capsid Proteins; D000939:Epitopes; C009496:H protein, Tobacco mosaic virus; D007130:Immunoglobulin Idiotypes; D014764:Viral Proteins; D008715:Methionine