title: Adhesion mutants of Dictyostelium discoideum lacking the saccharide determinant recognized by two adhesion-blocking monoclonal antibodies.
abstract: A mutant of Dictyostelium discoideum, strain HL260, was isolated based on its failure to bind d-41, a monoclonal antibody that blocks developmentally regulated cell-cell adhesion. The mutant fails to normally acquire cell-cell adhesion as assayed with cells shaken in 10 mM EDTA, but aggregates and and constructs fruiting bodies. Other mutant strains, HL216 and HL220, previously shown to have impaired cell-cell adhesion, also lack the determinant that binds d-41. The three strains all carry mutations in a gene designated mod B, which directs a post-translational modification of several developmentally regulated D. discoideum glycoproteins. Diploids formed between independent mod B mutant haploid strains also lack this determinant and show marked impairment of cell-cell adhesion in EDTA, indicating that mutations in mod B, rather than other mutations not shared by the haploid strains, are related to the adhesion defect. The results are consistent with other evidence that an oligosaccharide carried on several developmentally regulated glycoproteins plays an essential role in EDTA-resistant cell-cell adhesion in D. discoideum. However, this type of adhesion is not essential for morphogenesis in that the only defect detected thus far in mod B mutant strains is that they construct relatively smaller fruiting bodies that contain fewer spores.
authors.forename: W F
authors.initials: WF
authors.lastname: Loomis
authors.forename: S A
authors.initials: SA
authors.lastname: Wheeler
authors.forename: W R
authors.initials: WR
authors.lastname: Springer
authors.forename: S H
authors.initials: SH
authors.lastname: Barondes
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002448:Cell Adhesion / Q000187:drug effects; D002874:Chromosome Mapping; D004023:Dictyostelium / Q000235:genetics* / Q000254:growth & development; D004171:Diploidy; D004492:Edetic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis*; D008858:Microscopy, Phase-Contrast; D009154:Mutation; D009844:Oligosaccharides / Q000276:immunology*; D010641:Phenotype
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D009844:Oligosaccharides; D004492:Edetic Acid