Effect of priming with antigen on the expression of C3 receptors on murine B cells and the responses to TI-2 antigens.
The effect of priming with DNP-Ficoll on the expression of receptors for the third component of complement (CR) on B cells was studied. B cells from mice primed with antigen were separated into CR- and CR+ populations by rosetting and density gradient centrifugation, and they were examined for antibody responses to TI-2 antigens in vitro. CR- B cells in spleen cells from mice primed with antigen responded well in contrast with the low responsiveness of CR- B cells from unprimed mice. These CR- B cells from primed mice reached their peak responses 1 day earlier than CR+ B cells from primed or unprimed mice. The response of these CR- B cells was specific for the hapten used for priming, and the priming effect was most conspicuous in spleen cells from mice primed with antigen 3 days previously, and it decreased thereafter. The CR- B cells from primed mice also showed better responses than CR+ B cells to all polyclonal B cell activators (PBA) tested. When CR+ B cells were primed in vitro with DNP-Ficoll for 3 days, the major cell population responsive to the antigen in secondary culture was found to be CR- B cells. On the other hand, CR- B cells in spleens could not respond to DNP-Ficoll challenge after in vitro priming with the antigen for 3 days. These results indicate that B cells lose CR by priming with antigen, although they retain their responsiveness to antigens and PBA. The role of CR on B cells in the antibody response is discussed.
Nariuchi
H
H
Kakiuchi
T
T
Ueno
J
J
D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000008:administration & dosage*; D000955:Antigens, T-Independent / Q000276:immunology*; D001402:B-Lymphocytes / Q000145:classification / Q000276:immunology / Q000378:metabolism*; D016264:Dextran Sulfate; D003911:Dextrans / Q000276:immunology; D000939:Epitopes; D005260:Female; D005362:Ficoll / Q000031:analogs & derivatives / Q000276:immunology; D007700:Kinetics; D008212:Lymphocyte Depletion; D016177:Macrophage-1 Antigen; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011951:Receptors, Complement / Q000032:analysis*; D013154:Spleen / Q000166:cytology; D000511:alpha-Macroglobulins / Q000494:pharmacology
D000941:Antigens; D000955:Antigens, T-Independent; C031007:DNP-Ficoll; D003911:Dextrans; D000939:Epitopes; D016177:Macrophage-1 Antigen; D011951:Receptors, Complement; D000511:alpha-Macroglobulins; C032306:polyclonal B cell activator; D005362:Ficoll; D016264:Dextran Sulfate