title: An assay for GDP-D-mannose-4,6-dehydratase.
abstract: The mechanism of GDP-D-mannose-4,6-dehydratase action with respect to loss of the C5 hydrogen has been established using GDP-D-[5-3H]-mannose as a substrate. This observation has been incorporated into a rapid assay for the enzyme based on the equilibration of 3H with the aqueous medium.
authors.forename: S
authors.initials: S
authors.lastname: Chang
authors.forename: K O
authors.initials: KO
authors.lastname: Broschat
authors.forename: G S
authors.initials: GS
authors.lastname: Serif
mesh_terms: D006155:Guanosine Diphosphate Mannose; D006836:Hydro-Lyases / Q000378:metabolism*; D014316:Tritium; D014867:Water
chemical_list: D014867:Water; D014316:Tritium; D006155:Guanosine Diphosphate Mannose; D006836:Hydro-Lyases; C044971:GDPmannose 4,6-dehydratase