title: Chromatin-associated protein kinases in human normal and benign hyperplastic prostate.
abstract: Nuclear phosphoproteins and protein kinases of human normal and benign hyperplastic prostate (BPH) were studied in an effort to delineate their properties and to identify any underlying differences therein. Chromatin-associated protein kinases active towards phosvitin, lysine-rich histone, and endogenous nonhistone proteins were characterized in human prostatic nuclei. The general properties of the human chromatin-associated prostatic protein kinases were similar to those of rat ventral prostate chromatin. Polyamines stimulated the phosphorylation of endogenous nonhistone proteins and phosvitin. Protein kinases active towards phosvitin and lysine-rich histones were unaltered in chromatin from BPH tissue as compared with the normal prostate. However, phosphorylation of chromatin-associated nonhistone proteins was markedly enhanced (average, 123%) in BPH tissue as compared with the normal tissue. The results indicate a change in the protein kinase reaction specifically involving chromatin-associated nonhistone proteins of BPH tissue as compared with normal human prostate.
authors.forename: A
authors.initials: A
authors.lastname: Rayan
authors.forename: S A
authors.initials: SA
authors.lastname: Goueli
authors.forename: P
authors.initials: P
authors.lastname: Lange
authors.forename: K
authors.initials: K
authors.lastname: Ahmed
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D001345:Autoradiography; D002467:Cell Nucleus / Q000201:enzymology; D002843:Chromatin / Q000201:enzymology*; D004247:DNA / Q000302:isolation & purification; D013196:Dihydrotestosterone / Q000032:analysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D009698:Nucleoproteins / Q000032:analysis; D010766:Phosphorylation; D011467:Prostate / Q000201:enzymology* / Q000648:ultrastructure; D011470:Prostatic Hyperplasia / Q000201:enzymology*; D011494:Protein Kinases / Q000032:analysis*; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D002843:Chromatin; D009698:Nucleoproteins; D013196:Dihydrotestosterone; D012965:Sodium Chloride; D000255:Adenosine Triphosphate; D004247:DNA; D011494:Protein Kinases; D008274:Magnesium