Cross-linking of phosphatidylethanolamine neighbors with dimethylsuberimidate is sensitive to the lipid phase.
Dimethylsuberimidate was reacted with aqueous dispersions of dipalmitoylphosphatidylethanolamine, dimyristoylphosphatidylethanolamine, dilauroylphosphatidylethanolamine, and dielaidoylphosphatidylethanolamine at pH 10 and at pH 8. The amount of amidine dimer formation was about four times greater above the gel-to-fluid phase transition of each lipid than below the transition. The transition temperature of each phosphatidylethanolamine, measured by steady-state fluorescence anisotropy of cis-parinaric acid, was lower at pH 10 than at pH 8 or in water. The ability of dimethylsuberimidate to discriminate between phosphatidylethanolamines in the fluid and gel phases should allow use of this reagent to identify phosphatidylethanolamine species within the gel or fluid lipid phase.
Roth
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Division of Biology, Kansas State University, Manhattan 66506.
Avery
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Welti
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D003432:Cross-Linking Reagents; D004120:Dimethyl Suberimidate; D005231:Fatty Acids, Unsaturated; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes; D006863:Hydrogen-Ion Concentration; D007096:Imidoesters; D046911:Macromolecular Substances; D008560:Membrane Fluidity; D008563:Membrane Lipids; D010714:Phosphatidylethanolamines; D013696:Temperature; D014867:Water
D003432:Cross-Linking Reagents; D005231:Fatty Acids, Unsaturated; D005456:Fluorescent Dyes; D007096:Imidoesters; D046911:Macromolecular Substances; D008563:Membrane Lipids; D010714:Phosphatidylethanolamines; D014867:Water; D004120:Dimethyl Suberimidate; C007510:1,2-dipalmitoyl-3-phosphatidylethanolamine; C007445:1,2-dilauroylphosphatidylethanolamine; C020888:1,2-dielaidoylphosphatidylethanolamine; C013641:parinaric acid; C018525:1,2-dimyristoylphosphatidylethanolamine