Effect of phenethyl alcohol on Staphylococcus aureus alpha-lysin production.
Phenethyl alcohol, at the maximum concentration which did not inhibit growth (0.3% [vol/vol]), inhibited the production of alpha-lysin and exoproteases but not that of delta-lysin in Staphylococcus aureus Wood 46. The inhibition of alpha-lysin was reversible, and transient accumulation of cell-associated alpha-lysin occurred in the presence of PEA. A precursor of alpha-lysin ca. 3,000 daltons larger than extracellular alpha-lysin was immunologically detected in the sodium dodecyl sulfate extracts of membranes and whole cells of phenethyl alcohol-treated S. aureus cultures. Also, a degraded form of alpha-lysin was detected in membranes prepared from cells lysed by lysostaphin but not in membranes from cells lysed with an X-press.
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D000936:Antigen-Antibody Complex; D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000302:isolation & purification; D002462:Cell Membrane / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D000431:Ethanol / Q000031:analogs & derivatives*; D006460:Hemolysin Proteins; D007106:Immune Sera; D007700:Kinetics; D008970:Molecular Weight; D010626:Phenylethyl Alcohol / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000502:physiology*
D000936:Antigen-Antibody Complex; D001427:Bacterial Toxins; D006460:Hemolysin Proteins; D007106:Immune Sera; C021091:staphylococcal alpha-toxin; D000431:Ethanol; D010626:Phenylethyl Alcohol