title: NADH- and NADPH-linked aquacobalamin reductases occur in both mitochondrial and microsomal membranes of rat liver.
abstract: To elucidate the mechanism for the synthesis of the coenzyme forms of cobalamin in mammals, rat liver aquacobalamin reductase was partially characterized. Rat liver contained both NADH- and NADPH-linked aquacobalamin reductases. The NADH-linked enzyme was distributed in the mitochondria (approx. 40%) and microsomes (60%), identical to the distribution of the NADPH-linked enzyme. The two mitochondrial NADH- and NADPH-linked enzymes were located inside of the outer membrane.
authors.affiliation: Laboratory of Nutrition and Food Science, College of Hagoromo-gakuen, Osaka, Japan.
authors.forename: F
authors.initials: F
authors.lastname: Watanabe
authors.forename: Y
authors.initials: Y
authors.lastname: Nakano
authors.forename: S
authors.initials: S
authors.lastname: Maruno
authors.forename: N
authors.initials: N
authors.lastname: Tachikake
authors.forename: Y
authors.initials: Y
authors.lastname: Tamura
authors.forename: S
authors.initials: S
authors.lastname: Kitaoka
mesh_terms: D000818:Animals; D002458:Cell Fractionation; D007425:Intracellular Membranes / Q000201:enzymology*; D007700:Kinetics; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D008930:Mitochondria, Liver / Q000201:enzymology*; D009243:NAD / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000302:isolation & purification / Q000378:metabolism*; D009249:NADP / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013367:Submitochondrial Particles / Q000201:enzymology*
chemical_list: D009243:NAD; D009249:NADP; C053436:aquacobalamin reductase (NADPH); D009247:NADH, NADPH Oxidoreductases