title: CRP/cAMP- and CytR-regulated promoters in Escherichia coli K12: the cdd promoter.
abstract: Transcriptional regulation of the deoP2 promoter by the cyclic AMP/cyclic AMP receptor protein complex (cAMP/CRP) and the CytR repressor requires two high-affinity CRP targets located around -41 and -93 bp preceding the start site for transcription. Here we report the structure of cddP, another CRP/CytR-regulated promoter. In common with what was found in deo, the cdd promoter also contains multiple CRP targets. Thus, using the DNasel footprinting procedure, tandem CRP binding sites were identified around -41 and -93. These findings support a general model for CytR binding and CytR regulation, in which (i) CytR and the CRP/cAMP complex bind to similar or identical targets, (ii) two or more targets are necessary for proper binding of CytR to a promoter region, and (iii) CytR represses transcription by antagonizing cAMP/CRP activation.
authors.affiliation: Department of Molecular Biology, University of Odense, Denmark.
authors.forename: P
authors.initials: P
authors.lastname: Valentin-Hansen
authors.forename: B
authors.initials: B
authors.lastname: Holst
authors.forename: J
authors.initials: J
authors.lastname: Josephsen
authors.forename: K
authors.initials: K
authors.lastname: Hammer
authors.forename: B
authors.initials: B
authors.lastname: Albrechtsen
mesh_terms: D001483:Base Sequence; D003564:Cytidine Deaminase / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005796:Genes; D008969:Molecular Sequence Data; D009700:Nucleoside Deaminases / Q000235:genetics*; D009710:Nucleotide Mapping; D010957:Plasmids; D012150:Polymorphism, Restriction Fragment Length; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D014158:Transcription, Genetic
chemical_list: D009700:Nucleoside Deaminases; D003564:Cytidine Deaminase