Catecholamine measurements in urine by high-performance liquid chromatography with amperometric detection--comparison with an autoanalyser fluorescence method.
In order to validate different methods of measuring urinary catecholamines (norepinephrine, epinephrine and dopamine) in humans, methods based on separation of catecholamines using reversed-phase or cation-exchange high-performance liquid chromatography with electrochemical detection were compared with an autoanalyser-based fluorescence method. Different methods for pre-chromatography sample purification were also studied. For measurements of urinary catecholamines, the reversed-phase-based chromatographic techniques studied were found to give less reliable results than cation-exchange chromatography, even if one of them (Clin Rep Urine Catecholamine Kit) gave almost as precise estimates. The autoanalyser technique yielded good results. It is concluded that cation-exchange chromatography with an appropriate sample work-up procedure (a combination of organic solvent extraction and alumina adsorption) is a reliable and accurate method for analyses of urinary catecholamines.
Hjemdahl
P
P
Department of Clinical Pharmacology, Karolinska Hospital, Stockholm, Sweden.
Larsson
P T
PT
Bradley
T
T
Akerstedt
T
T
Anderzén
I
I
Sigurdsson
K
K
Gillberg
M
M
Lundberg
U
U
D000085:Acetates; D002395:Catecholamines / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange; D004563:Electrochemistry; D006586:Hexanes; D006801:Humans; D008297:Male; D011933:Reagent Kits, Diagnostic; D012997:Solvents; D013050:Spectrometry, Fluorescence
D000085:Acetates; D002395:Catecholamines; D006586:Hexanes; D011933:Reagent Kits, Diagnostic; D012997:Solvents; C007650:ethyl acetate