title: Effect of N-ethylmaleimide treatment on naloxone binding in frog brain membranes.
abstract: Specific binding of (3H) naloxone to opioid receptors in frog (Rana esculenta) brain membranes was irreversibly inactivated by the sulfhydryl group alkylating agent N-ethylmaleimide (NEM). Saturation analysis of (3H) naloxone binding revealed a marked reduction in the number of ligand binding sites after N-ethylmaleimide treatment. Pretreatment of the membranes with unlabelled opioid ligands, i.e. naloxone, morphine, or the kappa selective dynorphin (1-13), and sodium ions resulted in considerable protection of (3H) naloxone binding against the N-ethylmaleimide blockade.
authors.affiliation: Institute of Biochemistry, Hungarian Academy of Sciences, Szeged.
authors.forename: J
authors.initials: J
authors.lastname: Zawilska
authors.forename: A
authors.initials: A
authors.lastname: Lajtha
authors.forename: A
authors.initials: A
authors.lastname: Borsodi
mesh_terms: D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D004399:Dynorphins / Q000494:pharmacology; D005033:Ethylmaleimide / Q000494:pharmacology*; D066298:In Vitro Techniques; D007700:Kinetics; D008566:Membranes / Q000378:metabolism; D009020:Morphine / Q000494:pharmacology; D009270:Naloxone / Q000378:metabolism* / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology; D011893:Rana esculenta; D011957:Receptors, Opioid / Q000187:drug effects / Q000378:metabolism; D017473:Receptors, Opioid, kappa; D012964:Sodium / Q000494:pharmacology
chemical_list: D010446:Peptide Fragments; D011957:Receptors, Opioid; D017473:Receptors, Opioid, kappa; D009270:Naloxone; C026843:dynorphin (1-13); D004399:Dynorphins; D009020:Morphine; D012964:Sodium; D005033:Ethylmaleimide