Is terminal deoxynucleotidyl transferase an intracellular mutagen?
We have established stably transformed mammalian cell lines expressing recombinant human terminal deoxynucleotidyl transferase. A 58 kDa, enzymatically active protein is produced by these cell lines. Using the lacI gene of pJYMib shuttle vector as mutagenic target, we found no increase in mutation rates in cells expressing terminal deoxynucleotidyl transferase compared to controls. Our results suggest that the presence of terminal deoxynucleotidyl transferase alone in mammalian cells does not increase mutation rates.
Chang
L M
LM
Department of Biochemistry, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814.
Rusquet-Valerius
R
R
Roy
N K
NK
Cheung
L C
LC
Bollum
F J
FJ
D000818:Animals; D002460:Cell Line; D004253:DNA Nucleotidylexotransferase / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D005796:Genes; D006801:Humans; D008325:Mammary Neoplasms, Experimental / Q000201:enzymology; D051379:Mice; D009153:Mutagens; D010957:Plasmids; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000201:enzymology*
D009153:Mutagens; D004253:DNA Nucleotidylexotransferase