In vitro and in vivo action of diisopropylfluorophosphate, of atropine and their synergism on acetylcholinesterase activity.
Modifications of acetylcholinesterase (AChE) activity, caused by diisopropylfluorophosphate (DFP) and atropine and their synergism, were determined in vitro and in vivo in the rat. In vitro atropine caused a reduction of the AChE activity, but reduced the enzyme inhibition by DFP. These results are discussed in the light of the evidence that AChE is an allosteric enzyme. In vivo the AChE inhibition by DFP is in part reduced by atropine. The limited protection of AChE by atropine may be one of the factors implied in the reduction of the DFP toxicity by atropine.
Molinengo
L
L
Istituto di Farmacologia e Farmacognosia, Università di Torino, Italia.
Ghi
P
P
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001285:Atropine / Q000494:pharmacology*; D001921:Brain / Q000201:enzymology; D002417:Cattle; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D004357:Drug Synergism; D007531:Isoflurophate / Q000494:pharmacology*; D007701:Kinetin; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D002800:Cholinesterase Inhibitors; D007531:Isoflurophate; D001285:Atropine; D000110:Acetylcholinesterase; D007701:Kinetin