Assay for islet cell antibodies. Protein A--monoclonal antibody method.
Assays for islet cell antibodies (ICA) are finding increasing application in clinical diabetology. We have developed a new islet cell antibody assay system (ICA-pA), whose salient features include: (1) utilization of fluorescein-conjugated staphylococcal protein A as a standard second-step reagent, the advantages of this approach being improved "signal" (islet)/"noise" (acini) ratio due to reduction of interfering background acinar pancreatic staining, and facilitation of assay standardization provided by the use of a chemically pure conjugated protein A reagent; (2) monoclonal antibody counterstaining with rhodamine-conjugated BISL-32 for the rapid identification of islets in pancreatic sections; and (3) quantitation of circulating serum ICA by microimmunofluorometric techniques.
Srikanta
S
S
Rabizadeh
A
A
Omar
M A
MA
Eisenbarth
G S
GS
D000911:Antibodies, Monoclonal; D001323:Autoantibodies / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007515:Islets of Langerhans / Q000276:immunology*; D013194:Staining and Labeling; D013205:Staphylococcal Protein A
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D013205:Staphylococcal Protein A