Oral vaccination of rats with live avirulent Salmonella derivatives expressing adhesive fimbrial antigens of uropathogenic Escherichia coli.
The avirulent Salmonella typhimurium F885 was transformed with a plasmid carrying the cloned S fimbriae genes of a uropathogenic Escherichia coli. The resulting transformant (F885-1) produced efficiently E. coli S fimbriae and was used for live oral vaccination of rats. For comparison rats were immunized subcutaneously with isolated S fimbriae. Both routes of vaccination resulted in a significant IgG antibody response to S fimbriae. In addition live oral vaccination induced a serum IgA response against S fimbriae. After transurethral infection of rats with a S fimbriae producing E. coli a 10-fold reduction of bacterial counts in the kidney was observed in rats orally vaccinated with F885-1 as compared to unvaccinated controls. This study suggests that the avirulent Salmonella F885 may be used as a fimbrial antigen carrier for oral vaccination against renal infections.
Schmidt
G
G
Forschungsinstitut Borstel, F.R.G.
Hacker
J
J
Wood
G
G
Marre
R
R
D018830:Adhesins, Escherichia coli; D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology*; D004926:Escherichia coli / Q000276:immunology*; D005260:Female; D007070:Immunoglobulin A / Q000032:analysis; D007421:Intestine, Small / Q000382:microbiology; D007668:Kidney / Q000382:microbiology*; D051381:Rats; D012486:Salmonella typhimurium / Q000276:immunology*; D014169:Transformation, Bacterial; D014611:Vaccination; D014774:Virulence
D018830:Adhesins, Escherichia coli; D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D007070:Immunoglobulin A