Effect of antigastrin (SC-15396) on amylase release and 2-deoxyglucose uptake in mouse pancreatic acini.
The effects of antigastrin (SC-15396) on amylase release and 2-deoxyglucose uptake were studied in dispersed acini from mouse pancreas. Antigastrin at concentrations between 0.5 and 2 mM inhibited cholecystokinin (CCK)-stimulated amylase release and 2-deoxyglucose uptake in a dose-dependent fashion. Antigastrin at concentrations between 0.5 and 2 mM inhibited carbachol-stimulated amylase release and 2-deoxyglucose uptake as well. In addition, the drug (2 mM) inhibited bombesin-stimulated amylase release and 2-deoxyglucose uptake. In contrast, the stimulation of amylase release by Ca2+ ionophore A23187 was not inhibited by antigastrin. The results, therefore, suggest that antigastrin nonselectively inhibits the actions of these secretagogues probably at their receptor sites.
Iwamoto
Y
Y
Nakamura
R
R
Akanuma
Y
Y
Kuzuya
T
T
D000681:Amylases / Q000378:metabolism*; D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D001786:Blood Glucose / Q000378:metabolism*; D001839:Bombesin / Q000037:antagonists & inhibitors / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002217:Carbachol / Q000037:antagonists & inhibitors / Q000494:pharmacology; D046508:Culture Techniques; D003847:Deoxyglucose / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010179:Pancreas / Q000187:drug effects*; D011725:Pyridines / Q000494:pharmacology*; D012844:Sincalide / Q000037:antagonists & inhibitors / Q000494:pharmacology
D000897:Anti-Ulcer Agents; D001786:Blood Glucose; D011725:Pyridines; C004326:antigastrin; D000001:Calcimycin; D002217:Carbachol; D003847:Deoxyglucose; D000681:Amylases; D012844:Sincalide; D001839:Bombesin