A contiguous, 3-Mb physical map of Xq28 extending from the colorblindness locus to DXS15.
Using pulsed-field gel electrophoresis (PFGE), we have generated a 3-megabase (Mb) physical map of Xq28, a region of the human genome known to contain many disease loci. We have determined the location of the genes for protan/deutan colorblindness (R/GCP), factor VIII (F8), glucose-6-phosphate dehydrogenase (G6PD), and a series of RFLPs and have derived the following order for this region: R/GCP-GdX-G6PD-F8-DXS115-DXS33-DXS134-D XS15. Using newly isolated probes, we have also established the direction of transcription of F8 within the map and have localized CpG islands flanking this gene.
Kenwrick
S
S
Howard Hughes Medical Institute, University of California, San Francisco 94143-0724.
Gitschier
J
J
D015139:Blotting, Southern; D003117:Color Vision Defects / Q000235:genetics*; D015342:DNA Probes; D004587:Electrophoresis, Agar Gel; D008040:Genetic Linkage; D005819:Genetic Markers; D006801:Humans; D015183:Restriction Mapping; D014960:X Chromosome
D015342:DNA Probes; D005819:Genetic Markers