Different effects of ANP and nitroprusside on cyclic GMP extrusion of isolated aorta.
Atrial natriuretic peptide (ANP) and sodium nitroprusside have potent vasodilator effects on the noradrenaline-precontracted isolated rabbit aorta. A distinct elevation of cyclic GMP in the aortic tissue was observed after both vasodilators. In contrast to sodium nitroprusside, ANP-(5-28) induced a dose-dependent cyclic GMP extrusion from the tissue into the medium. Thus, release of cyclic GMP appears to be specific for activation of particulate guanylate cyclase and provides a mechanism in addition to synthesis and degradation by which the cells can regulate their internal concentrations of cyclic GMP.
Stasch
J P
JP
Bayer AG, Institute of Pharmacology, Wuppertal, F.R.G.
Kazda
S
S
Neuser
D
D
D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects / Q000378:metabolism; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D002682:Chinchilla; D000242:Cyclic AMP / Q000378:metabolism*; D005260:Female; D005292:Ferricyanides / Q000494:pharmacology*; D006162:Guanylate Cyclase / Q000378:metabolism; D066298:In Vitro Techniques; D009126:Muscle Relaxation / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism*; D009599:Nitroprusside / Q000494:pharmacology*; D009638:Norepinephrine / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology; D011817:Rabbits
D005292:Ferricyanides; D010446:Peptide Fragments; D009599:Nitroprusside; D009320:Atrial Natriuretic Factor; C054538:atrial natriuretic factor (5-28); D000242:Cyclic AMP; D006162:Guanylate Cyclase; D009638:Norepinephrine