Histochemical changes of substance P, FRAP, serotonin and succinic dehydrogenase in the spinal cord of rats with adjuvant arthritis.
Various histochemical changes were found in spinal segments L4-L5 of rats with adjuvant arthritis, predominantly 30 days after inoculation. A slight to marked increase of substance P immunoreactivity occurred in laminae I, II and X. FRAP activity was enhanced in lamina II. Serotonin immunoreactivity was heavier in laminae I, VIII and IX in a few animals. The intensity of the histoenzymological reaction for succinic dehydrogenase increased in certain laminae VIII and X neurons. At day 15 of the disease the increase of substance P and FRAP activities was chiefly restricted to the medial portion of the superficial dorsal horn. There was a significant positive correlation between the scratching behaviour of arthritic rats and the substance P immunoreactivity in laminae X and I. If one accepts that scratching is pain-related, the data are consistent with a possible role of substance P in the chronic pain associated with adjuvant arthritis. They leave undetermined the significance of the other histochemical changes.
Schoenen
J
J
Van Hees
J
J
Gybels
J
J
de Castro Costa
M
M
Vanderhaeghen
J J
JJ
D000135:Acid Phosphatase / Q000032:analysis*; D000818:Animals; D001168:Arthritis / Q000378:metabolism*; D001169:Arthritis, Experimental / Q000201:enzymology / Q000378:metabolism* / Q000523:psychology; D001522:Behavior, Animal; D005459:Fluorides / Q000494:pharmacology; D006651:Histocytochemistry; D007527:Isoenzymes / Q000032:analysis; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000032:analysis*; D013116:Spinal Cord / Q000032:analysis* / Q000201:enzymology; D013373:Substance P / Q000032:analysis*; D013385:Succinate Dehydrogenase / Q000032:analysis*; D013997:Time Factors
D007527:Isoenzymes; D012701:Serotonin; D013373:Substance P; D013385:Succinate Dehydrogenase; D000135:Acid Phosphatase; D005459:Fluorides