The response of rat alveolar macrophages to chronic inhalation of coal dust and/or diesel exhaust.
The use of diesel-powered equipment in underground mines has raised questions regarding possible synergistic effects of coal dust and diesel emissions. Therefore, the effects of chronic exposure of rats to coal dust and/or diesel exhaust on various properties of alveolar macrophages were investigated. Inhalation exposure of rats was 7 hr/day, 5 days/week for 2 years. Exposure groups were: filtered air controls, 2 mg/m3 coal dust, 2 mg/m3 diesel particulate, and 1 mg/m3 coal dust plus 1 mg/m3 diesel exhaust. Exposure to coal dust and/or diesel exhaust had little effect on oxygen consumption, membrane integrity, lysosomal enzyme activity, or protein content of alveolar macrophages. However, exposure to coal dust increased macrophage yield, enhanced chemiluminescence, and increased the activity of the cell membrane (i.e., increased cellular spreading and surface ruffling). In contrast, diesel emissions depressed chemiluminescence and decreased the ruffling of the cell membrane. Therefore, the data suggest that exposure to coal dust and/or diesel exhaust does not affect the viability of alveolar macrophages. However, coal dust may activate alveolar macrophages while diesel emissions may depress the phagocytic activity of these cells. The combination of exposures to coal dust and diesel exhaust results in a phagocytic activity which is an average of the effects of separate exposures.
Castranova
V
V
Bowman
L
L
Reasor
M J
MJ
Lewis
T
T
Tucker
J
J
Miles
P R
PR
D000818:Animals; D003031:Coal; D004391:Dust / Q000009:adverse effects*; D008163:Luminescent Measurements; D008247:Lysosomes / Q000201:enzymology; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D010101:Oxygen Consumption; D010587:Phagocytosis; D011506:Proteins / Q000032:analysis; D011650:Pulmonary Alveoli / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344; D001335:Vehicle Emissions / Q000633:toxicity*
D003031:Coal; D004391:Dust; D011506:Proteins; D001335:Vehicle Emissions