The hexose carrier from Chlorella. cDNA cloning of a eucaryotic H+-cotransporter.
The cDNA coding for the inducible H+/hexose cotransporter of Chlorella kessleri has been cloned and sequenced. It was isolated by differential screening of a cDNA library prepared from glucose-induced cells. The increase in expression of the gene correlates quantitatively with the increase in uptake activity due to induction; it is not expressed in a hexose transport mutant. An open reading frame allows for a membrane protein of 533 amino acids with a relative molecular mass of 57 kDa. This protein is highly homologous to the human and rat glucose transporters catalyzing facilitated diffusion and to the bacterial H+/pentose cotransporters. It is not related to the H+/lactose cotransporter of E. colli and to the mammalian Na+/glucose cotransporter.
Sauer
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Lehrstuhl für Zellbiologie und Pflanzenphysiologie, Universität Regensburg, FRG.
Tanner
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D001483:Base Sequence; D015139:Blotting, Southern; D002708:Chlorella / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D017343:Genes, Plant; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009004:Monosaccharide Transport Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics
D009004:Monosaccharide Transport Proteins; D012333:RNA, Messenger; D004247:DNA