Pregnancy-associated beta 1-macroglobulin: detection on the surface of ovarian cancer cell lines.
Pregnancy-associated beta 1-macroglobulin (beta 1-PAM) is a serum protein which can be detected at elevated levels in the blood of many ovarian cancer patients. The presence of the antigen was investigated on the surface of 4 human ovarian cancer lines and 32 other human and rodent cell types and lines by means of immunofluorescent staining and anti-beta 1-PAM-enzyme conjugate. The antigen was detected only on the ovarian lines but no secretion of beta 1-PAM into the culture supernatants was observed. The ovarian cancer cells appeared to display the beta 1-PAM constantly during culture, and enzymic treatment of the cells indicated that detection of the cell-bound antigen with antiserum could be, at least partially, eliminated with proteases but not glycosidases or neuraminidase. The results serve to confirm the association of beta 1-PAM with ovarian cancer.
Stimson
W H
WH
McAdam
A
A
Lang
G
G
Dodd
J
J
D000954:Antigens, Surface / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D010051:Ovarian Neoplasms / Q000032:analysis*; D011257:Pregnancy Proteins / Q000032:analysis*; D011268:Pregnancy-Specific beta 1-Glycoproteins / Q000032:analysis*
D000954:Antigens, Surface; D011257:Pregnancy Proteins; D011268:Pregnancy-Specific beta 1-Glycoproteins