title: Darkness stimulates rapid synthesis and release of melatonin in rat retina.
abstract: The presence of melatonin in retina has been widely reported for over two decades although studies of its functional importance within the retina have only recently been emphasized. We have analyzed the biochemical characteristics of melatonin synthesis and release, focusing on rapid changes in response to light/dark conditions. Our major findings are consistent with the following conclusions: (1) melatonin synthesis is stimulated within minutes after exposure to darkness, and may reflect an increase in N-acetyl transferase activity; (2) melatonin is not stored, but rather it diffuses freely throughout the retina immediately after it is synthesized; and (3) the dark-induced increase in retinal melatonin release is a synthesis-coupled response and does not involve separate secretion mechanisms. The characteristics of melatonin synthesis and release described herein would be consistent with the proposed role of melatonin as a local paracrine effector of dark-adaptive responses in retina.
authors.affiliation: Department of Neurobiology and Anatomy, University of Texas Medical School, Houston 77225.
authors.forename: D A
authors.initials: DA
authors.lastname: Redburn
authors.forename: C K
authors.initials: CK
authors.lastname: Mitchell
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003623:Dark Adaptation; D004058:Diffusion; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D008027:Light; D008550:Melatonin / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012160:Retina / Q000378:metabolism*; D012701:Serotonin / Q000031:analogs & derivatives / Q000378:metabolism; D014364:Tryptophan / Q000378:metabolism
chemical_list: D005971:Glutamates; D012701:Serotonin; D018698:Glutamic Acid; D014364:Tryptophan; D008550:Melatonin; C006389:N-acetylserotonin