Amplification of c-Ki-ras gene and aberrant expression of c-myc in WI-38 cells transformed in vitro by gamma-irradiation.
The expression of c-oncogenes was examined with normal human fibroblasts (WI-38) and the cells transformed in vitro by gamma-irradiation (CT-1). The amount of Ki-ras-specific mRNA in CT-1 cells was found to be approximately twice that in WI-38 cells. By Southern blot hybridization, the c-Ki-ras 2 gene of CT-1 cells was found to be amplified about two-fold. CT-1 cells have higher levels of c-myc mRNA than normal cells, although the gene dosage and the restriction nuclease pattern of the c-myc gene was the same. The content of c-myc mRNA in CT-1 cells was found to be constitutively high, whereas that in normal cells was increased by serum addition.
Mizuki
K
K
Nose
K
K
Okamoto
H
H
Tsuchida
N
N
Hayashi
K
K
D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000528:radiation effects*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; D005347:Fibroblasts; D005720:Gamma Rays; D005784:Gene Amplification; D005786:Gene Expression Regulation; D006801:Humans; D011061:Poly A / Q000032:analysis; D012313:RNA / Q000032:analysis; D012333:RNA, Messenger
D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII