title: Dot immunobinding as a tool for the study of the CNS myelin antigen, M2.
abstract: The unidentified antigen M2, which is the target of demyelinating antibodies in sera from guinea pigs immunized with brain tissue, was studied using a dot immunobinding assay. This method is particularly appropriate for studying the immunologic properties of insoluble brain components such as myelin, glycolipids and myelin proteins. M2 was found to be a component of central nervous system myelin and not peripheral myelin. It appeared at the time of myelination, was not cerebroside or GM1 ganglioside and did not cross-react with the major myelin proteins, i.e. proteolipid or basic protein. These observations confirm and extend previous studies using a complement fixation assay for M2. The antigen was shown to be partially solubilized by Brij 35 and this detergent can provide a starting point for attempts at purification.
authors.forename: R
authors.initials: R
authors.lastname: Lebar
authors.forename: M B
authors.initials: MB
authors.lastname: Lees
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology*; D001921:Brain / Q000254:growth & development / Q000276:immunology; D003902:Detergents; D006017:Glycolipids / Q000276:immunology; D006168:Guinea Pigs; D007074:Immunoglobulin G / Q000276:immunology; D004676:Myelin Basic Protein / Q000276:immunology; D009185:Myelin Proteins / Q000276:immunology; D009186:Myelin Sheath / Q000276:immunology*; D010525:Peripheral Nerves / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000941:Antigens; D003902:Detergents; D006017:Glycolipids; D007074:Immunoglobulin G; D004676:Myelin Basic Protein; D009185:Myelin Proteins