Identification and partial characterization of a T-cell-derived antigen-binding factor from mice infected with the intestinal helminth Trichinella spiralis.
Immunochemical and biological characterization was performed of an antigen-binding factor derived from culture supernatants of T cells from mice infected 4 days previously with the intestinal helminth Trichinella spiralis. Affinity chromatography with T. spiralis antigen resulted in the purification of a protein, provisionally designated Trichinella factor (Tric-F), that shared antigenic and other properties with a known T-cell-derived antigen-binding factor of different antigenic specificity, picryl chloride factor, which mediates an early 2-hour component of contact sensitivity. Tric-F lacked determinants of immunoglobulins and possessed determinants shared by other antigen-specific T cell factors, as determined by ELISA and antibody affinity chromatography. Biological activity of Tric-F was assayed in vivo and in vitro. Mice injected intravenously with Tric-F developed an antigen-specific early 2-hour ear swelling response following local challenge with T. spiralis antigen. These results corresponded to delayed-type hypersensitivity responses in the ears of T. spiralis-infected mice that comprised early 2-hour and late classical 24-hour responses. In vitro, Tric-F induced serotonin release by mast cells in the presence of T. spiralis antigen. Mast cells sensitized with Tric-F formed rosettes with antigen-coated sheep erythrocytes. It is suggested that Tric-F, an antigen-binding molecule that is T-cell-derived, mediates the early 2-hour component of delayed-type hypersensitivity and is involved in the initiation and regulation of T-cell-mediated intestinal inflammation during a T. spiralis infection in mice.
Parmentier
H K
HK
Department of Immunology, Faculty of Veterinary Medicine, State University of Utrecht, The Netherlands.
Dijkstra
J W
JW
Wissink
A
A
Ruitenberg
E J
EJ
Askenase
P W
PW
Van Loveren
H
H
D000818:Animals; D000947:Antigens, Helminth / Q000378:metabolism*; D002846:Chromatography, Affinity; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007111:Immunity, Cellular; D007073:Immunoglobulin E / Q000378:metabolism; D008407:Mast Cells / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D011485:Protein Binding; D012701:Serotonin / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology*; D014234:Trichinella / Q000276:immunology*; D014235:Trichinellosis / Q000276:immunology*
D000947:Antigens, Helminth; D012701:Serotonin; D007073:Immunoglobulin E