title: Transganglionic regulation of central terminals of dorsal root ganglion cells by nerve growth factor (NGF).
abstract: Blockade of axonal transport or transection of the rat sciatic nerve results in transganglionic degenerative atrophy (TDA) of nerve terminals containing fluoride-resistant acid phosphatase (FRAP) in the Rolando substance of the spinal cord. Application of vinblastine (9 micrograms) in a cuff around the sciatic nerve of adult rats blocked the retrograde transport of [125I]NGF in sensory fibers; this amount of vinblastine is identical to the threshold amount that induces TDA. Conversely, application of NGF to the proximal stump of the transected sciatic nerve prevented or delayed the occurrence of TDA as reflected by the maintenance of FRAP in the upper dorsal horn, that otherwise would inevitably disappear following the peripheral nerve lesion. These results suggest that endogenous NGF transported retrogradely in peripheral sensory fibers of the adult rat under normal conditions may be responsible for the regulation of the structural and functional integrity of the central terminals of these FRAP-containing primary sensory neurons and that TDA may be the consequence of the failure of NGF to reach the perikarya of these neurons.
authors.forename: B
authors.initials: B
authors.lastname: Csillik
authors.forename: M E
authors.initials: ME
authors.lastname: Schwab
authors.forename: H
authors.initials: H
authors.lastname: Thoenen
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D001370:Axonal Transport / Q000187:drug effects; D005727:Ganglia, Spinal / Q000502:physiology*; D008297:Male; D009410:Nerve Degeneration; D009414:Nerve Growth Factors / Q000378:metabolism / Q000502:physiology*; D009475:Neurons, Afferent / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D013376:Substantia Gelatinosa / Q000502:physiology; D014747:Vinblastine / Q000494:pharmacology
chemical_list: D009414:Nerve Growth Factors; D014747:Vinblastine; D000135:Acid Phosphatase