Immunoglobulin and T-cell receptor gene rearrangement. Implications for the diagnosis of lymphoproliferative disorders.
Recent studies have significantly broadened understanding of immunoglobulin production and T-cell receptor formation at the gene level. A limited number of genes can be rearranged during the course of B-cell or T-cell development to yield unique DNA sequences that will code for specific antibodies and T-cell receptor proteins. Southern blot hybridization analysis allows sensitive examination of lymphocyte DNA for the presence or absence of gene rearrangements. General mechanisms that underlie immunoglobulin and T-cell receptor rearrangement are reviewed, along with the diagnostic applications of detection of gene rearrangement by Southern blot hybridization technique.
Fishleder
A J
AJ
D015139:Blotting, Southern; D004247:DNA / Q000235:genetics; D015329:Gene Rearrangement, T-Lymphocyte; D006801:Humans; D006824:Hybridization, Genetic; D007136:Immunoglobulins / Q000235:genetics*; D008232:Lymphoproliferative Disorders / Q000175:diagnosis*; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*
D007136:Immunoglobulins; D011948:Receptors, Antigen, T-Cell; D004247:DNA