title: Activators of protein kinase C and 5-azacytidine induce IL-2 receptor expression on human T lymphocytes.
abstract: Resting human T lymphocytes do not express receptors for interleukin-2, but expression is rapidly induced by exposure to PHA. After maximal expression 2-3 days after stimulation, a progressive decline in receptor number is observed. Receptor expression can be augmented by reexposure to PHA. In this study we show that activators of protein kinase C including phorbol diester, phospholipase C, and the diacylglycerol congener diC8 also increase IL-2 receptor expression. Moreover, 5-azacytidine, which inhibits cytosine methyltransferase, and hydroxyurea, which inhibits ribonucleotide reductase, also increased receptor number. These studies demonstrate that IL-2 receptor expression can be altered in vitro, and that IL-2 receptor number, in combination with IL-2 secretion, may contribute to the regulation of IL-2-dependent immune responses.
authors.forename: J M
authors.initials: JM
authors.lastname: Depper
authors.forename: W J
authors.initials: WJ
authors.lastname: Leonard
authors.forename: C L
authors.initials: CL
authors.lastname: Drogula
authors.forename: M
authors.initials: M
authors.lastname: Krönke
authors.forename: T A
authors.initials: TA
authors.lastname: Waldmann
authors.forename: W C
authors.initials: WC
authors.lastname: Greene
mesh_terms: D001374:Azacitidine / Q000494:pharmacology*; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D008745:Methylation; D010835:Phytohemagglutinins / Q000494:pharmacology; D011493:Protein Kinase C; D011494:Protein Kinases / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000187:drug effects / Q000378:metabolism*; D015375:Receptors, Interleukin-2; D013601:T-Lymphocytes / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D010738:Type C Phospholipases / Q000494:pharmacology
chemical_list: D010835:Phytohemagglutinins; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2; D004247:DNA; D011494:Protein Kinases; D011493:Protein Kinase C; D010738:Type C Phospholipases; D001374:Azacitidine; D013755:Tetradecanoylphorbol Acetate