Hamster oocyte penetration tests with oocytes frozen in propanediol: comparison with non-frozen oocytes.
Hamster oocytes were frozen using a 1,2-propanediol-sucrose procedure, which resulted in over 90% survival. After thawing and zona removal the oocytes were compared with non-frozen oocytes in a zona-free hamster egg test employing spermatozoa from human semen donors and suspected infertility patients. Similar data were obtained, indicating that propanediol-sucrose frozen hamster eggs may be used in place of fresh eggs for convenience and to avoid scheduling problems.
Sachs
H H
HH
Reproductive and Developmental Biology, Research Institute and Gynecology, Cleveland Clinic Foundation, Ohio.
Pink
M J
MJ
Gwatkin
R B
RB
D000818:Animals; D006224:Cricetinae; D005260:Female; D005615:Freezing; D008297:Male; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D011309:Preservation, Biological; D011409:Propylene Glycols; D013084:Sperm-Ovum Interactions
D011409:Propylene Glycols