title: Silver staining of the nucleolar organizer regions (NORs) on Lowicryl and cryo-ultrathin sections.
abstract: Nucleolar organizer region (NOR) silver staining was applied to sections of fixed material. A positive reaction on cryo-ultrathin sections was found as well as on semithin and ultrathin Lowicryl sections. Repeatable staining that was easy to control was obtained by a one-step procedure after aldehyde-Carnoy fixation. Fixation of the material by formaldehyde and glutaraldehyde alone in cacodylate buffer also maintained reaction selectivity when ammonium chloride was used after fixation. Enzymatic digestion by pronase, RNase A, DNase I, or micrococcal nuclease was applied to ultrathin Lowicryl sections. Pronase digestion removed the silver-stained proteins, whereas digestion by the nucleases did not. A routine procedure is proposed for easy NOR silver staining of sections that preserves a good tissue ultrastructure and is also compatible with cytochemical and immunological investigations.
authors.forename: F J
authors.initials: FJ
authors.lastname: Moreno
authors.forename: D
authors.initials: D
authors.lastname: Hernandez-Verdun
authors.forename: C
authors.initials: C
authors.lastname: Masson
authors.forename: M
authors.initials: M
authors.lastname: Bouteille
mesh_terms: D000180:Acrylic Resins; D002460:Cell Line; D005629:Frozen Sections; D006651:Histocytochemistry; D006801:Humans; D009697:Nucleolus Organizer Region / Q000378:metabolism / Q000648:ultrastructure*; D012834:Silver; D013194:Staining and Labeling / Q000379:methods; D013997:Time Factors
chemical_list: D000180:Acrylic Resins; D012834:Silver; C037883:Lowicryl 4KM resin