Deletion mapping of DNA markers to a region of chromosome 5 that cosegregates with schizophrenia.
Two independent lines of evidence support the localization of a schizophrenia susceptibility locus to the proximal long arm of chromosome 5. A partial trisomy of chromosome 5 (5q11.2-q13.3) cosegregates with the disorder in a Canadian family of Chinese descent, and DNA markers from proximal 5q cosegregate with schizophrenia (plus related disorders) in families of British and Icelandic descent. We constructed a human:hamster hybrid cell line (HHW 1064) whose only human complement is a chromosome 5 that is missing the trisomic region associated with schizophrenia. In combination with a "matched" cell hybrid (HHW 105) containing an intact chromosome 5, we physically mapped DNA markers relative to the trisomy. "Schizophrenia-linked" DNA markers p105-153Ra (D5S39) and p105-599Ha (D5S76) map within the trisomy and proximal to the 5q11.2 breakpoint, respectively. The hybrid cell lines HHW 105 and HHW 1064 together provide a means to identify and generate syntenic DNA markers to further investigate the location of a schizophrenia locus.
Gilliam
T C
TC
Department of Psychiatry, College of Physicians and Surgeons, Columbia University, New York, New York 10032.
Freimer
N B
NB
Kaufmann
C A
CA
Powchik
P P
PP
Bassett
A S
AS
Bengtsson
U
U
Wasmuth
J J
JJ
D000818:Animals; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002895:Chromosomes, Human, Pair 5; D006224:Cricetinae; D005260:Female; D008040:Genetic Linkage; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D008297:Male; D009693:Nucleic Acid Hybridization; D010375:Pedigree; D012559:Schizophrenia / Q000235:genetics*; D014314:Trisomy
D005819:Genetic Markers