Onset of transcription of the aminopeptidase N (leukemia antigen CD 13) gene at the crypt/villus transition zone during rabbit enterocyte differentiation.
The sequence of a cDNA clone (2.82 kbp) of rabbit intestinal aminopeptidase N (CD 13) is reported. Using the corresponding anti-sense RNA probe, the distribution of aminopeptidase N mRNA along the crypt/villus axis of the rabbit small intestine was studied by in situ hybridization. The aminopeptidase N gene is expressed along the whole length of the villus with a maximum at its base. Expression was not detected in the crypt cells. The distribution of aminopeptidase N mRNA correlates with the presence of active enzyme as monitored by histochemical staining. The results are compatible with onset of transcription of the aminopeptidase N gene at the crypt/villus transition zone during the enterocyte differentiation.
Norén
O
O
Department of Biochemistry C, University of Copenhagen, Denmark.
Dabelsteen
E
E
Høyer
P E
PE
Olsen
J
J
Sjöström
H
H
Hansen
G H
GH
D000595:Amino Acid Sequence; D000626:Aminopeptidases / Q000235:genetics*; D000818:Animals; D015214:Antigens, Differentiation, Myelomonocytic / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D018826:CD13 Antigens; D002454:Cell Differentiation; D015971:Gene Expression Regulation, Enzymologic; D007421:Intestine, Small / Q000166:cytology / Q000502:physiology*; D008871:Microvilli / Q000201:enzymology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015347:RNA Probes; D011817:Rabbits; D014158:Transcription, Genetic
D015214:Antigens, Differentiation, Myelomonocytic; D015347:RNA Probes; D000626:Aminopeptidases; D018826:CD13 Antigens