Concanavalin A binding sites in fetal, adult, transitional, and malignant rectosigmoid mucosa.
We studied mucin histochemistry in 25 rectosigmoid adenocarcinomas and in the transitional mucosa adjacent to these tumors using standard techniques for the detection of neutral and acid sialomucins and sulfomucins and the paradoxical concanavalin A (Con A) stain. This histochemical procedure selectively detects residues of mannose in glycoproteins exposed to brief steps of oxidation and reduction. Those techniques were also used to study histologically normal mucosa of specimens with carcinoma, normal rectosigmoid mucosa of patients without inflammatory or neoplastic bowel disease, hyperplastic rectal polyps, and rectosigmoid mucosa of human fetuses. Normal mucosa and hyperplastic polyps mainly contained sulfomucins and did not display Con A binding activity with any of the variants of the stain. In contrast, fetal, transitional, and malignant mucosa predominantly showed sialomucins and although not reactive with the standard Con A sequence, displayed binding activity for the lectin after short oxidative-reductive steps. These results provide further evidence that transitional and malignant mucosa produce markedly abnormal mucins whose histochemical patterns represent a re-emergence of the fetal type found during development. The principles of the paradoxic Con A reaction may be applied to unmask lectin binding activity in apparently unreactive sites.
Caccamo
D
D
Department of Pathology, Hospital Italiano, Buenos Aires, Argentina.
Telenta
M
M
Celener
D
D
D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D000368:Aged; D001665:Binding Sites; D003208:Concanavalin A / Q000032:analysis*; D005260:Female; D005333:Fetus; D006801:Humans; D008297:Male; D008875:Middle Aged; D009092:Mucous Membrane / Q000378:metabolism / Q000473:pathology; D011247:Pregnancy; D012004:Rectal Neoplasms / Q000378:metabolism / Q000473:pathology*; D012007:Rectum / Q000473:pathology*; D012811:Sigmoid Neoplasms / Q000378:metabolism / Q000473:pathology*
D003208:Concanavalin A