title: Specificity of antibodies for T sites and F sites of streptolysin O.
abstract: Antistreptolysin antibody (ASO) inhibits haemolysis of erythrocytes coated with streptolysin O (SLO), but antistreptolysin activity may also be due to the presence in sera of peptide fragments of altered beta lipoproteins. SLO-mediated haemolysis results from activity of a molecular site (the t site) distinct from that at which the SLO becomes attached to the cell (the f site), and both sites might be expected to function antigenically. Absorption studies with SLO-coated latex particles, and with SLO-coated erythrocytes in the cold, provide evidence for the existence of both classes of antibody. Results also suggest explanations for the frequent observation of false-negative and false-positive latex tests.
authors.forename: K C
authors.initials: KC
authors.lastname: Watson
authors.forename: E J
authors.initials: EJ
authors.lastname: Kerr
mesh_terms: D000918:Antibody Specificity; D001426:Bacterial Proteins; D001666:Binding Sites, Antibody; D016264:Dextran Sulfate; D003911:Dextrans / Q000494:pharmacology; D004912:Erythrocytes / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006384:Hemagglutination / Q000187:drug effects; D006461:Hemolysis; D006801:Humans; D008934:Mitogens / Q000494:pharmacology; D013301:Streptolysins / Q000276:immunology*
chemical_list: D001426:Bacterial Proteins; D003911:Dextrans; D008934:Mitogens; D013301:Streptolysins; C025964:streptolysin O; D016264:Dextran Sulfate