title: Acetylcholine receptor expression in primary cultures of embryonic chick myotubes--I. Discoordinate regulation of alpha-, gamma- and delta-subunit gene expression by calcitonin gene-related peptide and by muscle electrical activity.
abstract: The neuropeptide calcitonin gene-related peptide calcitonin gene-related peptide, and muscle electrical activity regulate in opposite directions the content of nicotinic acetylcholine receptor alpha-subunit mRNA in primary cultures of chick embryonic myotubes. Indeed, treating the cells with calcitonin gene-related peptide or blocking the spontaneous activity of muscle cells by tetrodotoxin (an inhibitor of sodium channels) increases, although to different levels, the content of acetylcholine receptor alpha-subunit mRNA [Fontaine B., Klarsfeld A. and Changeux J. P. (1987) J. Cell Biol. 105, 1337-1342; Klarsfeld A. and Changeux J. P. (1985) Proc. natn. Acad. Sci. U.S.A. 82, 4558-4562]. In this paper, we demonstrate that, under these in vitro culture conditions, calcitonin gene-related peptide (0.1 microM) and tetrodotoxin (0.5 microM) regulate to a smaller extent (no more than 2.5-fold above control) the levels of acetylcholine receptor gamma- and delta-subunit mRNAs. No effect of either compound on acetylcholine receptor biosynthesis was observed during the initial three days of culture. The response to calcitonin gene-related peptide was already maximal when the cells were treated between days three and four after plating (about 3-fold increase of the alpha-subunit mRNA level). The effect of tetrodotoxin resulted in a six-fold increase of the acetylcholine receptor alpha-subunit mRNA level in cells treated between days three and four, and still increased when the cells were exposed to tetrodotoxin through days six and eight (up to a maximum of 20-fold).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: URA CNRS 0210, Département des Biotechnologies, Institut Pasteur, Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Osterlund
authors.forename: B
authors.initials: B
authors.lastname: Fontaine
authors.forename: A
authors.initials: A
authors.lastname: Devillers-Thiery
authors.forename: B
authors.initials: B
authors.lastname: Geoffroy
authors.forename: J P
authors.initials: JP
authors.lastname: Changeux
mesh_terms: D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology*; D002478:Cells, Cultured; D002642:Chick Embryo; D005314:Embryonic and Fetal Development; D005786:Gene Expression Regulation / Q000187:drug effects*; D009132:Muscles / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D011950:Receptors, Cholinergic / Q000235:genetics* / Q000502:physiology; D013779:Tetrodotoxin / Q000494:pharmacology
chemical_list: D012333:RNA, Messenger; D011950:Receptors, Cholinergic; D013779:Tetrodotoxin; D015740:Calcitonin Gene-Related Peptide