Human liver cytochrome P-450 related to a rat acetone-inducible, nitrosamine-metabolizing cytochrome P-450: identification and isolation.
A monoclonal antibody (MAb) to a rat acetone-inducible and nitrosamine-metabolizing form of microsomal cytochrome P-450, P-450ac, detected a related P-450 in human liver microsomes by both immunoblot and competitive radioimmunoassay. This MAb was also used to immunopurify microsomal cytochromes P-450 from both human liver and acetone-treated rats; these were electrophoretically homogeneous with apparent molecular weights of 56,200 and 53,000 daltons, respectively. The structures of the cytochromes P-450 were compared by peptide mapping and amino-terminal sequence analyses. They differed in their peptide maps but displayed amino-terminal sequence similarity in their first 19 residues. This report thus demonstrates the utility of MAbs to rat cytochromes P-450 for detection, identification and structural characterization of human P-450s.
Robinson
R C
RC
Laboratory of Molecular Carcinogenesis, National Cancer Institute, Bethesda, Md.
Shorr
R G
RG
Varrichio
A
A
Park
S S
SS
Gelboin
H V
HV
Miller
H
H
Friedman
F K
FK
D000096:Acetone / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D003577:Cytochrome P-450 Enzyme System / Q000302:isolation & purification*; D006801:Humans; D015151:Immunoblotting; D008099:Liver / Q000032:analysis*; D008297:Male; D008862:Microsomes, Liver / Q000032:analysis; D008969:Molecular Sequence Data; D009602:Nitrosamines / Q000378:metabolism; D010449:Peptide Mapping; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains
D000911:Antibodies, Monoclonal; D009602:Nitrosamines; D000096:Acetone; D003577:Cytochrome P-450 Enzyme System