Immune response to a thymus-independent antigen (DNP-Ficoll) in the guinea pig.
Guinea pigs immunized with DNP30-Ficoll produced IgM antibody only. No IgG1, IgG2, IgE antibodies or delayed hypersensitivity were detected in these animals. However, Arthus reactions, induced by the hapten coupled to a foreign carrier or the whole antigen, were found. The time course of the IgM response was limited and the response to reinjection of the antigen reduced. Cyclophosphamide (CY), given 3 days before primary immunization, prolonged the IgM response. Given on the day of immunization or 3 days after CY reduced this response. CY given on days +3 or +7 after primary immunization completely suppressed the response to antigen reinjected 42 days later. Arthus reactions were totally suppressed by CY given on the day of immunization, or 3 or 7 days later.
Drössler
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Parker
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Turk
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D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D000941:Antigens; D002417:Cattle / Q000276:immunology; D003520:Cyclophosphamide / Q000494:pharmacology; D005260:Female; D005362:Ficoll / Q000031:analogs & derivatives / Q000276:immunology*; D006168:Guinea Pigs; D007117:Immunization, Secondary; D007075:Immunoglobulin M / Q000276:immunology; D008297:Male; D011134:Polysaccharides / Q000276:immunology*; D012882:Skin Tests; D005719:gamma-Globulins / Q000276:immunology
D000941:Antigens; C031007:DNP-Ficoll; D007075:Immunoglobulin M; D011134:Polysaccharides; D005719:gamma-Globulins; D005362:Ficoll; D003520:Cyclophosphamide