[Cytological analysis of the protein component of the nuclear chromatin in rat sympathetic neurocytes in postnatal ontogeny. I. The age-related changes in the histones detectable by ammoniacal silver and the matrix activity of the chromatin in rat sympathetic neurocytes].
The nuclear histones of the rat superior cervical ganglion neurons were stained at different stages of postnatal ontogenesis with ammoniacal silver (AS). Considerable changes in neuronal staining were revealed during the terminal cytodifferentiation, whereas no changes were found during mature age and in a more advanced age. A combined method was proposed that involved simultaneous AS-staining and radioautographical evaluation of transcription in the same cell. No correlation was seen between the nuclear AS-staining and the number of active transcription units.
Grigor'eva
A V
AV
Iarygin
V N
VN
D000375:Aging; D000818:Animals; D001345:Autoradiography; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure*; D005728:Ganglia, Sympathetic / Q000378:metabolism / Q000648:ultrastructure*; D006657:Histones / Q000378:metabolism*; D008099:Liver / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron; D009474:Neurons / Q000378:metabolism / Q000648:ultrastructure*; D009562:Nissl Bodies / Q000378:metabolism / Q000648:ultrastructure*; D051381:Rats; D012834:Silver; D013194:Staining and Labeling / Q000379:methods; D014158:Transcription, Genetic
D006657:Histones; C000212:ammoniacal silver; D012834:Silver
Tsitologicheskiĭ analiz belkovogo komponenta iadernogo khromatina simpaticheskikh nevrotsitov krysy v postnatal'nom ontogeneze. I. Vozrastnye izmeneniia gistonov, vyiavliaemye ammiachnym serebrom, i matrichnaia aktivnost' khromatina simpaticheskikh nevrotsitov krysy.