Modulation of a 190-kD microtubule-associated protein in pigment epithelium by VIP.
Vasoactive intestinal peptide stimulates phosphorylation of six high molecular weight cytosolic proteins in the cultured retinal pigment epithelium (RPE). Of these, the 190-kD phosphoprotein is associated with the microtubules assembled by taxol/GTP and is immunologically related to the brain microtubule-associated protein 2 (mol.wt. = 280 kD). VIP is also shown here to stimulate secretion in the cultured RPE. VIP-stimulated phosphorylation of a 190-kD microtubule-associated protein is also demonstrated here in the retinal glia.
Koh
S W
SW
Department of Ophthalmology, University of Maryland, Baltimore 21201.
D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D003600:Cytosol / Q000378:metabolism; D005136:Eye Proteins / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000494:pharmacology; D015151:Immunoblotting; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008970:Molecular Weight; D009457:Neuroglia / Q000378:metabolism; D017239:Paclitaxel; D010766:Phosphorylation; D010857:Pigment Epithelium of Eye / Q000378:metabolism*; D014660:Vasoactive Intestinal Peptide / Q000502:physiology*
D000470:Alkaloids; D005136:Eye Proteins; D008869:Microtubule-Associated Proteins; D014660:Vasoactive Intestinal Peptide; D006160:Guanosine Triphosphate; D017239:Paclitaxel