Interzeta-globin gene hypervariable regions in Chinese: application to genetic diagnosis of adult polycystic kidney disease.
In order to investigate the interzeta-globin gene hypervariable regions (IZHVR) in Chinese, DNA samples from 114 unrelated normal individuals were analyzed using the restriction endonuclease BglII and hybridized with a 32P-labeled zeta-globin probe. Four polymorphic fragments containing IZHVR with different frequencies were found: 11.3 kb, 0.24; 10.8 kb, 0.50; 10.4 kb, 0.05, and 10.0 kb, 0.21. Using these polymorphic fragments as linkage markers, we were able to trace the affected siblings in a three-generation Chinese family with a history of adult polycystic kidney disease.
Wann
S
S
Department of Medicine, Veterans General Hospital, Taipei, Taiwan, Republic of China.
Chen
T W
TW
Huang
T P
TP
Wang
J Y
JY
Peng
H W
HW
D002681:China; D005260:Female; D008040:Genetic Linkage; D005914:Globins / Q000235:genetics*; D006801:Humans; D008297:Male; D007690:Polycystic Kidney Diseases / Q000175:diagnosis* / Q000235:genetics; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
D005914:Globins