High-performance liquid chromatography of deacetylmetipranolol in plasma.
A sensitive high-performance liquid chromatographic method with electro-chemical detection is developed for the determination of deacetylmetipranolol (DMP), an active metabolite of beta-sympatholytic drug metipranolol (MP). DMP is extracted from the plasma after basifying with dichloromethane. After evaporation, the residue is reconstituted in the mobile phase, injected onto the reversed-phase ODS column and chromatographed at 50 degrees C. The sensitivity of the method is 2 ng of DMP in 1 ml plasma. Pindolol, another beta-blocking agent is used as internal standard. The method is used for a pharmacokinetic investigation of MP in healthy volunteers and in the patients with liver cirrhosis.
Tkaczyková
M
M
State Institute for Control of Drugs, Praha, Czechoslovakia.
Safarík
L
L
D000319:Adrenergic beta-Antagonists / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004563:Electrochemistry; D006801:Humans; D007202:Indicators and Reagents; D014290:Metipranolol / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D000319:Adrenergic beta-Antagonists; D007202:Indicators and Reagents; C006204:deacetyltrimepranol; D014290:Metipranolol