An ELISA method for the quantification of anti-streptolysin-O antibodies.
This paper describes a new assay, based on the ELISA technique, for the quantification of antibodies to streptolysin-O (ASLO). We have compared its performances with that of a standard method (inhibition of hemolysis). Using a panel of 137 sera covering the whole range of ASLO titers, the results showed a good correlation between both methods but the ELISA method was more reproducible than the standard technique, thus represents a convenient alternative for the quantification of ASLO.
Moscoso del Prado
J
J
Departamento de Investigación, Alergia e Inmunología Abelló, S.A., Madrid, Spain.
Serrano
C
C
D000907:Antibodies, Bacterial / Q000032:analysis*; D001426:Bacterial Proteins; D004797:Enzyme-Linked Immunosorbent Assay; D006461:Hemolysis; D006801:Humans; D015203:Reproducibility of Results; D012213:Rheumatic Fever / Q000175:diagnosis*; D013301:Streptolysins / Q000032:analysis* / Q000276:immunology
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D013301:Streptolysins; C025964:streptolysin O