The efficiency of 1 M NH4Cl and 2 M NaCl for the isolation of pathogenic Nocardia from soil.
The present paper reports an improvement to the classical method of the paraffin bait, by the usage of 1 M NH4Cl or 2 M NaCl to eliminate contaminant microflora of soil. The purpose is to introduce a change in the paraffin bait method in order to reduce time required to isolate pathogenic strains of Nocardia from their natural sources. For this study three main criteria were used: a) Determination of the inhibitory effect of different concentrations of salts on soil microflora; b) The tolerance of Nocardia brasiliensis, Nocardia asteroides and Nocardia caviae (Nocardia otitidis caviarum) strains to these chemical inhibitors; c) Determination of the efficiency of salts in the isolation of Nocardia from soil when strains are grown on paraffin baits.
van Gelderen de Komaid
A
A
Instituto de Microbiología Dr. Luis C. Verna, Facultad de Bioquimica, Universidad Nacional de Tucumán, República Argentina.
Durán
E L
EL
D000643:Ammonium Chloride / Q000494:pharmacology*; D015169:Colony Count, Microbial; D006801:Humans; D009615:Nocardia / Q000187:drug effects / Q000302:isolation & purification*; D010232:Paraffin; D012965:Sodium Chloride / Q000494:pharmacology*; D012988:Soil Microbiology
D000643:Ammonium Chloride; D012965:Sodium Chloride; D010232:Paraffin