Specificity of antisera obtained to substance P and its C-terminal hexapeptide.
Synthetic fragments and analogs were used to characterize specificity of antisera to SP and SP6-11. [Tyr8] SP and [Lys6] SP6-11 were both used as radioiodinated ligands. The latter was conjugated with Bolton-Hunter reagents before labelling. In both systems, the C-terminal pentapeptide SP7-11 was the shortest fragment showing antigenic identity with Substance P molecule. Substitution of different amino acid residues in SP6-11 by His or Gly showed that all but Glu6 take part in the structure of the antigenic determinant.
Babinska
A
A
Cierniewski
C S
CS
Koziolkiewicz
W
W
Janecka
A
A
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D001667:Binding, Competitive; D000939:Epitopes / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology*; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D011817:Rabbits; D012710:Serum Albumin, Bovine; D013373:Substance P / Q000276:immunology*
D000939:Epitopes; D010446:Peptide Fragments; D012710:Serum Albumin, Bovine; D013373:Substance P; C032292:substance P (6-11); D011761:Pyrrolidonecarboxylic Acid