Increase in intracellular cysteine after exposure to dithiothreitol: implications in radiobiology.
Dithiothreitol reduces cystine in tissue-culture medium, and the resulting cysteine is accumulated in Chinese hamster V79 cells to an extent which should be taken into account in evaluating models of radiosensitivity and the effects of thiol radioprotectors.
Dennis
M F
MF
Gray Laboratory of the Cancer Research Campaign, Mount Vernon Hospital, Northwood, Middlesex, U.K.
Stratford
M R
MR
Wardman
P
P
Watfa
R R
RR
D000818:Animals; D006224:Cricetinae; D003545:Cysteine / Q000378:metabolism*; D004229:Dithiothreitol / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D066298:In Vitro Techniques; D011836:Radiation Tolerance / Q000502:physiology*; D011837:Radiation-Protective Agents / Q000494:pharmacology; D013268:Stimulation, Chemical
D011837:Radiation-Protective Agents; D003545:Cysteine; D004229:Dithiothreitol