Induction of microsomal 1-acylglycerophosphocholine acyltransferase by peroxisome proliferators in rat kidney; co-induction with peroxisomal beta-oxidation.
Induction of microsomal 1-acyl-glycerophosphocholine (GPC) acyltransferase in rat tissues by four peroxisome proliferators, clofibric acid, tiadenol, DEHP and PFOA, was examined. Among the nine tissues examined, kidney, liver and intestinal mucosa responded to the challenges by the peroxisome proliferators to induce the enzyme. The treatment of rats with various dose of clofibric acid, tiadenol, DEHP or PFOA resulted in an induction of kidney microsomal 1-acyl-GPC acyltransferase in a dose-dependent manner. Despite the structural dissimilarity of peroxisome proliferators, the induction of microsomal 1-acyl-GPC acyltransferase was highly correlated with the induction of peroxisomal beta-oxidation. The activity of microsomal 1-acyl-GPC acyltransferase was not affected by changes in hormonal (adrenalectomy, diabetes, hyperthyroidism and hypothyroidism) and nutritional (starvation, starvation-refeeding, fat-free-diet feeding and high-fat-diet feeding) states. The induction of renal microsomal 1-acyl-GPC acyltransferase was seen in mice subsequent to the administration of clofibric acid and tiadenol and in guinea pigs subsequent to the administration of tiadenol. These results may indicate that kidney microsomal 1-acyl-GPC acyltransferase is a highly specific parameter responsive to the challenges by peroxisome proliferators and may suggest that the possibility that the inductions by peroxisome proliferators of microsomal 1-acyl-GPC acyltransferase and peroxisomal beta-oxidation in kidney are co-regulated.
Kawashima
Y
Y
Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.
Matsunaga
T
T
Hirose
A
A
Ogata
T
T
Kozuka
H
H
D008243:1-Acylglycerophosphocholine O-Acyltransferase; D000217:Acyltransferases / Q000096:biosynthesis*; D000818:Animals; D002210:Caprylates / Q000494:pharmacology; D002995:Clofibric Acid / Q000494:pharmacology; D004051:Diethylhexyl Phthalate / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D005233:Fatty Alcohols / Q000494:pharmacology; D005466:Fluorocarbons / Q000494:pharmacology; D006168:Guinea Pigs; D007668:Kidney / Q000201:enzymology* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008830:Microbodies / Q000187:drug effects / Q000378:metabolism*; D008861:Microsomes / Q000201:enzymology*; D008862:Microsomes, Liver / Q000201:enzymology; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains
D002210:Caprylates; D005233:Fatty Alcohols; D005466:Fluorocarbons; C004568:tiadenol; D002995:Clofibric Acid; C023036:perfluorooctanoic acid; D004051:Diethylhexyl Phthalate; D000217:Acyltransferases; D008243:1-Acylglycerophosphocholine O-Acyltransferase