Isoprenaline induced changes in ornithine decarboxylase activity and polyamine content in regions of the rat heart.
Isoprenaline stimulated increases in ornithine decarboxylase activity and polyamine content in cardiac tissues are implicated in macromolecular synthesis and cellular growth, but little is known about polyamine metabolism in functionally distinct regions of the heart. We therefore determined regional changes in ornithine decarboxylase activity and polyamine content in the right and left ventricles, septum, and the right and left atria of the rat following the administration of isoprenaline. An increase in ornithine decarboxylase specific activity and tissue polyamine content occurred in all cardiac regions, but the highest ornithine decarboxylase activity was found in the septum. Propranolol inhibited the isoprenaline stimulated ornithine decarboxylase activity in all the regions. Putrescine increased and peaked between 6 and 8 h in right and left ventricles and septum and declined to a control level by 12 h. Following a peak increase at 8 h, spermidine and spermine contents of both ventricles were maintained at peak levels, while those in the septum declined to control values by 12 h. There was no detectable putrescine in the right atria from the control experiments and in either atrium at 2 h following isoprenaline administration. Putrescine content peaked at 6 h in the right atrium and at 8 h in the left atrium and then declined. In both atria there was a peak increase in spermidine and spermine contents between 4 and 8 h. These results show that there is a regional variation in the accumulation of polyamines in the rat heart following isoprenaline administration.
Tipnis
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UR
Department of Pharmacology, University of Texas Medical School, Houston 77225.
Frasier-Scott
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Skiera
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D000818:Animals; D004789:Enzyme Activation / Q000187:drug effects; D006321:Heart / Q000187:drug effects*; D007545:Isoproterenol / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D011073:Polyamines / Q000032:analysis*; D011433:Propranolol / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013268:Stimulation, Chemical
D011073:Polyamines; D011433:Propranolol; D009955:Ornithine Decarboxylase; D007545:Isoproterenol