A 6000 kb segment of chromosome 1 is conserved in human and mouse.
A murine linkage map generated from analyses of 428 meiotic events in an interspecific cross and pulsed field gel electrophoresis allowed examination of the genomic organization of a 6000 kb segment of mouse and human chromosome 1. Analysis of five genes within this syntenic segment of both species revealed striking conservation of gene order, intergenic distance and, to a lesser extent, CpG dinucleotides. In the mouse, meiotic crossover events were not evenly distributed; a hot spot for meiotic recombination was coincident with a CpG-island. These studies provide a practical approach to aid physical mapping of the human genome and a model for determining the molecular principles that govern meiotic recombination. In addition, these findings demonstrate profound conservation of genomic organization over mammalian evolution.
Kingsmore
S F
SF
Department of Medicine, Duke University Medical Center, Durham, NC 27710.
Watson
M L
ML
Howard
T A
TA
Seldin
M F
MF
D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D003433:Crosses, Genetic; D015342:DNA Probes; D005260:Female; D005796:Genes; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H / Q000235:genetics; D009115:Muridae / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D011995:Recombination, Genetic; D015183:Restriction Mapping
D015342:DNA Probes