Molecular cloning and characterization of a gene coding for methanol oxidase in Hansenula polymorpha.
The structural gene and the regulatory DNA sequence of the yeast Hansenula polymorpha methanol oxidase have been isolated. According to the nucleotide sequence data obtained, the structural gene encodes a 664 amino acids long protein, contains no intervening sequences, and the 5'- and 3'-non-coding region contains several sequences implicated in transcription initiation and termination in the yeast Saccharomyces cerevisiae. Although the methanol oxidase is translocated to the peroxisomes, no cleavable signal sequence was found at the N-terminus of the protein.
Ledeboer
A M
AM
Edens
L
L
Maat
J
J
Visser
C
C
Bos
J W
JW
Verrips
C T
CT
Janowicz
Z
Z
Eckart
M
M
Roggenkamp
R
R
Hollenberg
C P
CP
D000429:Alcohol Oxidoreductases / Q000235:genetics*; D000595:Amino Acid Sequence; D001203:Ascomycota / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004720:Endonucleases / Q000378:metabolism; D009690:Nucleic Acid Conformation; D010843:Pichia / Q000201:enzymology / Q000235:genetics*; D011061:Poly A / Q000032:analysis; D014176:Protein Biosynthesis; D012313:RNA / Q000032:analysis; D012333:RNA, Messenger; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014158:Transcription, Genetic
D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D000429:Alcohol Oxidoreductases; C029293:methanol oxidase; D004720:Endonucleases; D004262:DNA Restriction Enzymes; D015719:Single-Strand Specific DNA and RNA Endonucleases