Isolation of populations of antipeptide antibodies directed against different epitopes of the same fragment.
Rabbit antibodies against small peptides may be composed by subpopulations recognizing different epitopes made likely by few amino acids. This explains the frequent crossreactivity of antipeptide antibodies with unrelated peptides. A suitable use of immunoadsorbents is suggested to obtain truly specific antibodies able to react with restricted amino acid sequences.
Chersi
A
A
Greger
C
C
Houghten
R A
RA
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D000918:Antibody Specificity; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis*; D007164:Immunosorbents; D010455:Peptides / Q000276:immunology*; D011817:Rabbits
D000906:Antibodies; D000939:Epitopes; D007164:Immunosorbents; D010455:Peptides