A double fluorescence method for determining doxorubicin distribution and vascular supply in the mouse kidney.
Freeze-sectioning followed by freeze-drying completely preserves the topographic distribution of soluble substances, with the additional advantage of permitting permanent mounting. This rapid and effective technique was applied to study the distribution of fluorescence of the cytotoxic drug Doxorubicin in microscopic slices from in vivo treated animals. A fluorescent conjugate of different fluorescence, Blankophor-G-Dextran, which remained in the vessels, was used in the same living animals to characterize the vascular supply in the kidney of treated mice in relation to Doxorubicin distribution.
Paschoud
N
N
Bignell
C
C
Reinhold
H S
HS
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004317:Doxorubicin / Q000378:metabolism*; D005456:Fluorescent Dyes; D005612:Freeze Drying; D005629:Frozen Sections; D006651:Histocytochemistry / Q000379:methods; D007668:Kidney / Q000098:blood supply* / Q000378:metabolism; D007678:Kidney Glomerulus / Q000098:blood supply; D007700:Kinetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence / Q000379:methods; D013194:Staining and Labeling; D014230:Triazoles
D005456:Fluorescent Dyes; D014230:Triazoles; C043962:Blankophor G; D004317:Doxorubicin