Peroxidase-labeled primary antibody method for detection of pestivirus contamination in cell cultures.
Contaminating bovine viral diarrhea (BVD) virus in cell cultures and in fetal bovine serum (FBS) is a well recognized problem. This study describes a direct peroxidase (DP) labeled primary antibody method for detection of pestivirus antigens in cell culture that is simple and reliable. Using this method, most bovine and porcine cell cultures, a cat lung, four mosquito and two monkey cell cultures were found contaminated with BVD virus. The rodent and human cell cultures tested were negative by this method for BVD virus.
Potts
B J
BJ
Infectious Diseases Branch, National Institute of Neurological and Communicative Disorders and Stroke, Bethesda, MD 20892.
Sawyer
M
M
Shekarchi
I C
IC
Wismer
T
T
Huddleston
D
D
D000818:Animals; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000032:analysis*; D001769:Blood / Q000382:microbiology*; D002415:Cats; D002417:Cattle / Q000382:microbiology; D002478:Cells, Cultured / Q000382:microbiology*; D002522:Chlorocebus aethiops; D009033:Culicidae; D046508:Culture Techniques / Q000379:methods; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D010576:Pestivirus / Q000302:isolation & purification*; D012377:Rodentia; D012756:Sheep / Q000276:immunology; D013552:Swine
D000914:Antibodies, Viral; D000956:Antigens, Viral