title: Regulation of steroidogenesis and cholesterol synthesis by prostaglandin F-2 alpha and lipoproteins in bovine luteal cells.
abstract: Bovine luteal cells can utilize low density lipoprotein (LDL) or high density lipoprotein (HDL) as a source of cholesterol for steroidogenesis, and administration of PGF-2 alpha in vitro suppresses lipoprotein utilization. The objective of this study was to examine the mechanism by which PGF-2 alpha exerts this effect. Cultured bovine luteal cells received 0.25 microCi[14C]acetate/ml, to assess rates of de-novo sterol and steroid synthesis, with or without lipoproteins. Both LDL and HDL enhanced progesterone production (P less than 0.01), but caused a significant reduction in the amount of radioactivity in the cholesterol fraction. PGF-2 alpha treatment inhibited the increase in lipoprotein-induced progesterone synthesis (P less than 0.01), but did not prevent the reduction in de-novo cholesterol synthesis brought about by LDL or HDL. PGF-2 alpha alone reduced cholesterol synthesis (P less than 0.01), but it was not as effective as either LDL or HDL. Both lipoproteins and PGF-2 alpha also decreased the amount of radioactivity in the progesterone fraction (P less than 0.01), and the effect of PGF-2 alpha was similar to that of the lipoproteins. It is concluded that lipoproteins can enhance progesterone production and also suppress de-novo cholesterol synthesis in bovine luteal cells, but only the former effect of lipoproteins is inhibited by PGF-2 alpha. Therefore, it is suggested that PGF-2 alpha allows entry of lipoprotein cholesterol into the cell, but prevents utilization for steroidogenesis. In addition, PGF-2 alpha alone can suppress cholesterol synthesis, as well as decrease conversion of cholesterol to progesterone.
authors.affiliation: Department of Dairy Science, Ohio State University, Columbus 43210.
authors.forename: J L
authors.initials: JL
authors.lastname: Pate
authors.forename: W A
authors.initials: WA
authors.lastname: Condon
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D002784:Cholesterol / Q000096:biosynthesis*; D003338:Corpus Luteum / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D015237:Dinoprost / Q000494:pharmacology*; D005260:Female; D008075:Lipoproteins, HDL / Q000494:pharmacology*; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D011374:Progesterone / Q000096:biosynthesis*
chemical_list: D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D011374:Progesterone; D002784:Cholesterol; D015237:Dinoprost