title: NGF receptor reexpression and NGF-mediated cholinergic neuronal hypertrophy in the damaged adult neostriatum.
abstract: Adult cholinergic interneurons of the neostriatum are not immunoreactive for monoclonal antibody to NGF receptor, whereas the developing neostriatum is immunoreactive for this same antibody. Chronic NGF infusion into the adult neostriatum resulted in reexpression of the NGF receptor such that many cholinergic interneurons became immunoreactive for NGF receptor. NGF infusion dramatically increased the size and choline acetyltransferase immunoreactivity of these same cholinergic neurons. Additionally, in situ hybridization demonstrated an increase in the number of cells expressing NGF receptor mRNA in the NGF-infused striatum. These findings indicate that central cholinergic neurons which lose their NGF receptors during postnatal development will resume their NGF responsiveness when the tissue is damaged. Such a damage-induced mechanism may act to enhance the action of trophic factors, including NGF, released at the site of injury and enhance the responsiveness of damaged CNS neurons to exogenously administered trophic factors.
authors.affiliation: Department of Neuroscience, University of California, San Diego, La Jolla 92093.
authors.forename: F H
authors.initials: FH
authors.lastname: Gage
authors.forename: P
authors.initials: P
authors.lastname: Batchelor
authors.forename: K S
authors.initials: KS
authors.lastname: Chen
authors.forename: D
authors.initials: D
authors.lastname: Chin
authors.forename: G A
authors.initials: GA
authors.lastname: Higgins
authors.forename: S
authors.initials: S
authors.lastname: Koh
authors.forename: S
authors.initials: S
authors.lastname: Deputy
authors.forename: M B
authors.initials: MB
authors.lastname: Rosenberg
authors.forename: W
authors.initials: W
authors.lastname: Fischer
authors.forename: A
authors.initials: A
authors.lastname: Bjorklund
mesh_terms: D000375:Aging; D000818:Animals; D000911:Antibodies, Monoclonal; D002795:Choline O-Acetyltransferase / Q000378:metabolism*; D003342:Corpus Striatum / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D009414:Nerve Growth Factors / Q000378:metabolism / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000235:genetics*; D017475:Receptors, Nerve Growth Factor; D012016:Reference Values; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D005904:Glial Fibrillary Acidic Protein; D009414:Nerve Growth Factors; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D017475:Receptors, Nerve Growth Factor; D014446:Tyrosine 3-Monooxygenase; D002795:Choline O-Acetyltransferase