[Method of isolating FMRF-amide-like substances in the snail, using affinity chromatography coupled with HPLC].
An extraction procedure of FMRFa-like substances from brain of Helix aspersa was developed. It consists of using affinity chromatography coupled with reverse phase HPLC. Three synthetic peptides (FMRFa, pQDPFLRFa, Met-enkephalin) were used to evaluate the specificity and yield of the affinity column. Its efficiency was tested by use of snail brain extracts. The results showed that this method is efficient and reproducible.
Mounzih
K
K
U.R.A. CNRS 687, Faculté des Sciences, Besançon, France.
Marchand
C R
CR
Griffond
B
B
D000818:Animals; D001923:Brain Chemistry; D002846:Chromatography, Affinity / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004744:Enkephalin, Methionine / Q000302:isolation & purification; D019835:FMRFamide; D006372:Helix, Snails / Q000032:analysis*; D009479:Neuropeptides / Q000302:isolation & purification*; D018377:Neurotransmitter Agents / Q000302:isolation & purification*
D009479:Neuropeptides; D018377:Neurotransmitter Agents; D004744:Enkephalin, Methionine; D019835:FMRFamide
Mise au point d'une méthode d'isolement des substances FMRF-amide-like de l'Escargot, utilisant la chromatographie d'affinité couplée à la CLHP.