title: Dissection of Mycobacterium tuberculosis antigens using recombinant DNA.
abstract: A recombinant DNA strategy has been used systematically to survey the Mycobacterium tuberculosis genome for sequences that encode specific antigens detected by monoclonal antibodies. M. tuberculosis genomic DNA fragments with randomly generated endpoints were used to construct a large lambda gt11 recombinant DNA expression library. Sufficient numbers of recombinants were produced to contain inserts whose endpoints occur at nearly every base pair in the pathogen genome. Protein antigens specified by linear segments of pathogen DNA and produced by the recombinant phage of Escherichia coli were screened with monoclonal antibody probes. This approach was coupled with an improved detection method for gene isolation using antibodies to clonally isolate DNA sequences that specify polypeptide components of M. tuberculosis. The methodology described here, which is applicable to other pathogens, offers possibilities for the development of more sensitive and specific immunodiagnostic and seroepidemiological tests for tuberculosis and, ultimately, for the development of more effective vaccines.
authors.forename: R A
authors.initials: RA
authors.lastname: Young
authors.forename: B R
authors.initials: BR
authors.lastname: Bloom
authors.forename: C M
authors.initials: CM
authors.lastname: Grosskinsky
authors.forename: J
authors.initials: J
authors.lastname: Ivanyi
authors.forename: D
authors.initials: D
authors.lastname: Thomas
authors.forename: R W
authors.initials: RW
authors.lastname: Davis
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000235:genetics*; D003001:Cloning, Molecular; D003090:Coliphages; D004269:DNA, Bacterial / Q000235:genetics; D004274:DNA, Recombinant; D000939:Epitopes / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D004269:DNA, Bacterial; D004274:DNA, Recombinant; D000939:Epitopes