The antigenic characteristics and the significance of the adenine nucleotide translocator as a major autoantigen to antimitochondrial antibodies in dilated cardiomyopathy.
To obtain further knowledge of the antigen-antibody system, immunochemical characterization of the adenine nucleotide translocator was achieved by crossed immunoelectrophoresis, immunoreplica technique, radioimmunoassay, immunoabsorption studies, and nucleotide-transport measurements. Sera of 18 patients with proven congestive cardiomyopathy (CCM) were tested. On the adenine nucleotide translocator (ANT) from heart, kidney, and liver, organ-specific antigenic determinants were demonstrable, although a partial cross-reactivity existed. Conformation specificity was also confirmed by experimental studies. Of the patients studied, 17 of 18 with CCM showed a significant binding to the heart ANT, while no or a lower binding was seen on the kidney/liver ANT. In CCM, a correlation exists between the ejection fraction and the anti-ANT titer. These results give new evidence for autoimmunological events in MC and CCM and indicate a possible causal relationship between these two diseases.
Schultheiss
H P
HP
Schwimmbeck
P
P
Bolte
H D
HD
Klingenberg
M
M
D000328:Adult; D000368:Aged; D000941:Antigens / Q000276:immunology*; D001323:Autoantibodies / Q000032:analysis; D001324:Autoantigens / Q000276:immunology*; D002311:Cardiomyopathy, Dilated / Q000276:immunology*; D000939:Epitopes / Q000276:immunology*; D005260:Female; D006333:Heart Failure / Q000276:immunology*; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D007668:Kidney / Q000276:immunology; D008099:Liver / Q000276:immunology; D008297:Male; D008875:Middle Aged; D008929:Mitochondria, Heart / Q000276:immunology*; D000226:Mitochondrial ADP, ATP Translocases / Q000276:immunology*; D009206:Myocardium / Q000276:immunology; D009713:Nucleotidyltransferases / Q000276:immunology*; D009928:Organ Specificity; D011863:Radioimmunoassay
D000941:Antigens; D001323:Autoantibodies; D001324:Autoantigens; D000939:Epitopes; D000226:Mitochondrial ADP, ATP Translocases; D009713:Nucleotidyltransferases