[Effect of amphotericin B added to one side of a membrane].
Amphotericin B which was added to the one side of a membrane in aqueous solutions with low pH 3.0 sharply increases the membrane conductance by the mechanism of channel forming. Long living ionic channels (20 sec) with large conductance dispersion (2-20 Ps) were found at the concentration 2 X 10(-8) M. Amphotericin B concentration which is necessary for obtaining single channels does not depend on cholesterol concentration in the membrane-forming solution in the range from 2 to 10 mg/ml. The effect of amphotericin B on the membrane depends on the phospholipid concentration in the membrane. The one-side selective permeability is the same as one under the symmetric action of the antibiotic. It is assumed that amphotericin B induced the membrane conductance by the mechanism of half-pores formation.
Kasumov
Kh M
KhM
Karakozov
S D
SD
D000666:Amphotericin B / Q000494:pharmacology*; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism*; D008051:Lipid Bilayers; D008563:Membrane Lipids / Q000378:metabolism; D008566:Membranes / Q000378:metabolism; D010539:Permeability; D010743:Phospholipids / Q000378:metabolism
D007473:Ion Channels; D008051:Lipid Bilayers; D008563:Membrane Lipids; D010743:Phospholipids; D000666:Amphotericin B
Effekt amfoteritsina B, vvodimogo s odnoĭ storony membrany.