Translation in a cell-free system, and in Xenopus oocytes, of mRNA which specify a cadmium-binding protein in Nereis diversicolor (Annelida, Polychaeta).
The presence in the marine worm Nereis diversicolor of a low molecular mass protein with the capacity to bind cadmium has been previously demonstrated. Poly(A)(+)-mRNA were extracted from coelomocytes of Nereis diversicolor and were translated either in vitro, using a rabbit reticulocyte lysate, or in vivo into Xenopus laevis oocytes. Analysis of synthesized polypeptides by enzyme-linked immunosorbent assay (ELISA) and by Western blotting, using a specific monoclonal anti-MP II antibody, showed that this metalloprotein was translated both in in vitro and in vivo translation systems, with an apparent molecular mass of 11-13 kDa. Two other products, with 26.5 and 28 kDa molecular mass, cross-reacted with the monoclonal anti-MP II antibodies. The present work confirms that coelomocytes are sites of important synthesis of MP II-mRNA.
Benouareth
D E
DE
CNRS UA 148, Université de Lille-Flandres-Artois, Villeneuve-d'Ascq, France.
Dhainaut-Courtois
N
N
Porchet-Hennere
E
E
Curgy
J J
JJ
D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D002104:Cadmium / Q000378:metabolism*; D002474:Cell-Free System / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D007124:Immunoenzyme Techniques; D008668:Metallothionein / Q000096:biosynthesis*; D009865:Oocytes / Q000378:metabolism; D011061:Poly A / Q000235:genetics; D011077:Polychaeta / Q000166:cytology / Q000235:genetics*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D014982:Xenopus laevis
D000911:Antibodies, Monoclonal; D012333:RNA, Messenger; C004203:cadmium-binding protein; D002104:Cadmium; D011061:Poly A; D008668:Metallothionein