title: Hormonal and paracrine regulation of gamma-glutamyl transpeptidase in rat Sertoli cells.
abstract: The effects of follicle-stimulating hormone (FSH) and testosterone on Sertoli cell gamma-glutamyl transpeptidase (gamma-GTP) activity have been studied in vitro. Addition of FSH to Sertoli cell cultures for 5 days induced stimulation of gamma-GTP activity. No testosterone effect was observed alone or in combination with different doses of FSH. Time course studies for a supramaximal dose of FSH showed that enzyme induction could be achieved after a 48 h stimulation. Furthermore, a gradual stimulation of gamma-GTP activity in response to increasing numbers of germinal cells (GC) added in coculture, was observed. Stimulation was also demonstrated with germinal cell-conditioned medium (GCCM). Stimulatory effects of GC and GCCM were additive with those of FSH, suggesting that different mechanisms were involved.
authors.affiliation: Centro de Investigaciones Endocrinologicas, Hospital de Ninos R. Gutierrez, Buenos Aires, Argentina.
authors.forename: H F
authors.initials: HF
authors.lastname: Schteingart
authors.forename: M A
authors.initials: MA
authors.lastname: Rivarola
authors.forename: S B
authors.initials: SB
authors.lastname: Cigorraga
mesh_terms: D000818:Animals; D001141:Aromatase / Q000032:analysis; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012708:Sertoli Cells / Q000187:drug effects / Q000201:enzymology*; D013090:Spermatocytes / Q000378:metabolism; D013268:Stimulation, Chemical; D013739:Testosterone / Q000494:pharmacology*; D005723:gamma-Glutamyltransferase / Q000378:metabolism*
chemical_list: D003470:Culture Media; D013739:Testosterone; D005640:Follicle Stimulating Hormone; D001141:Aromatase; D005723:gamma-Glutamyltransferase