A simple method for rapid and precise estimation of the protamine dose required for clinical heparin reversal.
A simple, colorimetric protamine titration method was developed for rapid and precise estimation of the protamine dose required for clinical heparin reversal. The method employs azure A dye as the titration indicator, and has replaced the time consuming clotting assay in the conventional protamine titration method with a rapid colorimetric assay. It offers the same accuracy in estimating the protamine dose as that of the conventional titration method, but allows the processing time to be shortened to within 5 min. In a similar manner, the colorimetric assay can also be employed with a heparin titration procedure to quickly assess the heparin dose required for protamine reversal. This would allow physicians to exercise a quick and accurate heparin back-titration to patients who are overdosed with protamine.
Yang
V C
VC
College of Pharmacy, University of Michigan, Ann Arbor 48109-1065.
D002315:Cardiopulmonary Bypass; D003124:Colorimetry / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D006493:Heparin / Q000008:administration & dosage; D006494:Heparin Antagonists; D006801:Humans; D011479:Protamines / Q000008:administration & dosage*; D013053:Spectrophotometry
D006494:Heparin Antagonists; D011479:Protamines; D006493:Heparin