BLV-p24 expression in BLV infected cattle and detection of BLV-p24 receptors in cattle afflicted with tumorous leukosis in vivo.
Noncultivated and short-term cultivated blood leukocytes of BLV-infected cattle both with and without tumorous leukosis have been investigated for BLV-p24 antigen expression and for their capacity to bind BLV-p24 antigen. In animals with persistent lymphocytosis using cell extracts of 3-6 X 10(8) non-short-term cultivated blood leukocytes, BLV-p24 antigen could be identified by means of competitive RIA. In cattle with tumorous leukosis, the antigen detection in non-short-term cultivated leukocytes may be masked by the presence of antigen-binding receptors. The capacity of p24 antigen binding is likely to be a phenotypical marker for the cells occurring in the tumour phase only.
Burkhardt
H
H
Humboldt-University, Institute of Virology, Berlin, G.D.R.
Ristau
E
E
Rosenthal
S
S
Müller
M
M
D000818:Animals; D000957:Antigens, Viral, Tumor / Q000096:biosynthesis*; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D002478:Cells, Cultured; D007457:Iodine Radioisotopes; D007938:Leukemia / Q000382:microbiology / Q000662:veterinary*; D001909:Leukemia Virus, Bovine / Q000276:immunology*; D007962:Leukocytes / Q000032:analysis; D011485:Protein Binding; D011863:Radioimmunoassay; D011991:Receptors, Virus / Q000032:analysis*; D012190:Retroviridae / Q000276:immunology*
D000957:Antigens, Viral, Tumor; D007457:Iodine Radioisotopes; D011991:Receptors, Virus