title: Rapid myosin phosphorylation transients in phasic contractions in chicken gizzard smooth muscle.
abstract: In intact smooth muscle strips from chicken gizzard, electrical stimulation and carbachol elicited brief, phasic contractions which were associated with a very rapid, transient phosphorylation of the 20 kDa myosin light chains. The phosphorylation transients reached their peak after 3 s and 6 s and preceded that of force. Phosphorylation was not significantly different from basal levels after 10 s and 30 s while force still amounted to 50% of the peak value. The rate of tension decline could be increased by cessation of stimulation or by addition of atropine, even at apparently basal phosphorylation levels suggesting a phosphorylation independent regulation.
authors.affiliation: II. Physiologisches Institut, Universität Heidelberg, FRG.
authors.forename: W
authors.initials: W
authors.lastname: Fischer
authors.forename: G
authors.initials: G
authors.lastname: Pfitzer
mesh_terms: D000818:Animals; D001285:Atropine / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D002645:Chickens; D004558:Electric Stimulation; D005895:Gizzard, Avian / Q000187:drug effects / Q000378:metabolism; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000378:metabolism*; D015879:Myosin Subfragments / Q000378:metabolism; D009218:Myosins / Q000378:metabolism*; D010766:Phosphorylation
chemical_list: D015879:Myosin Subfragments; D001285:Atropine; D002217:Carbachol; D009218:Myosins