Isolation of specific antigen-reactive T-cell clones from nude (nu/nu) mice infected with Mesocestoides corti.
Infection of nude mice with Mesocestoides corti results in spleen enlargement, with a decrease in percentage positive, but overall increase in Thy-1 positive cells. A low frequency of both parasite antigen-specific and alloantigen-reactive T-cell clones were isolated from the spleen, mesenteric lymph nodes, peritoneal exudate and Peyer's patches. The T-cell clones from spleen were found to express the antigens Thy-1, Lyt-1 and Lyt-2; furthermore, after stimulation with antigen in the absence of exogenous T cell growth factor (IL-2), they produced normal levels of haemopoietic growth factor (IL-3), bone marrow proliferation activity and eosinophil differentiation activity. A higher proportion produced IL-2 compared to clones from normal mice. The isolation of antigen-specific T-cell clones showing normal T-cell characteristics from young nude mice adds weight to suggestions that exposure to antigen results in significant extra-thymic T-cell maturation.
Sanderson
C J
CJ
Strath
M
M
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D000954:Antigens, Surface / Q000032:analysis; D002590:Cestode Infections / Q000276:immunology*; D002999:Clone Cells; D000939:Epitopes; D007376:Interleukin-2 / Q000032:analysis; D007518:Isoantibodies / Q000032:analysis; D051379:Mice; D008819:Mice, Nude; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D018800:Thy-1 Antigens
D000947:Antigens, Helminth; D000954:Antigens, Surface; D000939:Epitopes; D007376:Interleukin-2; D007518:Isoantibodies; C029805:Lyt antibodies; D018800:Thy-1 Antigens