Platelet-activating factor (PAF) acts as an intercellular messenger in the changes of cytosolic free Ca2+ in human neutrophils induced by opsonized particles.
Addition of opsonized particles to human neutrophils in suspension leads to a biphasic elevation in the cytosolic free Ca2+ concentration ([Ca2+]i). The rise in [Ca2+]i during the second phase (greater than 3 min) is pronounced (about 400 nM), in contrast to the rise during the first phase, which is relatively small (less than 100 nM). The second and large rise in [Ca2+]i is brought about by messenger(s) released from the cell after addition of opsonized particles. This second rise in [Ca2+]i is not observed in the presence of the platelet-activating factor (PAF) antagonist WEB 2086, indicating that PAF can act as an intercellular messenger affecting Ca2+ homeostasis in human neutrophils.
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Central Laboratory of the Netherlands Red Cross, Blood Transfusion Service, University of Amsterdam, The Netherlands.
Verhoeven
A J
AJ
Roos
D
D
Koenderman
L
L
D001381:Azepines / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D009504:Neutrophils / Q000502:physiology*; D010587:Phagocytosis; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors / Q000502:physiology*; D015398:Signal Transduction; D014227:Triazines / Q000494:pharmacology; D014230:Triazoles; D015054:Zymosan
D001381:Azepines; D010972:Platelet Activating Factor; D014227:Triazines; D014230:Triazoles; C052518:WEB 2086; D015054:Zymosan; D002118:Calcium