[Factors affect Na(+)-Ca2+ exchange in rat's cardiomyocytes and protein-reconstituted liposomes].
Rat's Ca-resistant ventricular myocytes and reconstituted liposomes of canine cardiac sarcolemma were used in analysing factors affecting Na(+)-Ca2+ exchange system. Decrease in extracellular (extravesicular) Na+ or increase in intracellular Na+ by inhibition of the Za(+)-K(+)-ATPase with ouabain stimulated the Na(+)-Ca2+ exchange. Mn2+, an antagonist of Na(+)-Ca2+ exchange, dose-dependently inhibited the Na(+)-dependent Ca2+ uptake. However, verapamil had no effect on it. Arachidonic acid and lipid peroxides caused by incubation with-H2O2 significantly stimulated Na(+)-Ca2+ exchange in a dose-dependent fashion.
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D000818:Animals; D001095:Arachidonic Acids / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D066298:In Vitro Techniques; D007474:Ion Exchange; D008081:Liposomes / Q000378:metabolism; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D012964:Sodium / Q000378:metabolism*
D001095:Arachidonic Acids; D008081:Liposomes; D012964:Sodium; D002118:Calcium