Amplification and expression of a cellular oncogene (c-myc) in human gastric adenocarcinoma cells.
Three of 16 human gastric adenocarcinoma samples, maintained as solid tumors in nude mice, were found to carry amplified c-myc genes. In two samples with a high degree of c-myc DNA amplification (15- to 30-fold), double minute chromosomes were observed in karyotype analysis. The level of c-myc RNA was markedly elevated in a rapidly growing and poorly differentiated tumor, whereas it was only slightly elevated in a slowly growing and more differentiated tumor.
Shibuya
M
M
Yokota
J
J
Ueyama
Y
Y
D000230:Adenocarcinoma / Q000235:genetics*; D000818:Animals; D004587:Electrophoresis, Agar Gel; D005784:Gene Amplification; D005786:Gene Expression Regulation; D006801:Humans; D007621:Karyotyping; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009857:Oncogenes; D012313:RNA / Q000032:analysis; D013274:Stomach Neoplasms / Q000235:genetics*
D012313:RNA