CD2 expression in murine B cell lineage.
CD2 expression in murine B cell lineage was examined by flow cytometric and immunoprecipitation studies with anti-murine CD2 mAb and by Northern blot analysis. Cell surface expression of CD2 was demonstrated on all peripheral B cells and cell lines of B lineage. The murine CD2 transcript of 1.3 kb was detected in these B cells. An identical glycoprotein of 55-67 KD was precipitated from the lysates of surface radioiodinated thymocytes, splenic T and B cells, T and B lymphomas, RL male 1 and BCL-1, with anti-murine CD2 mAb. The majority of bone marrow B cells and a half of pre-B cells were found to be positive for CD2 expression. These results indicate that murine CD2 is expressed on B cell lineage at certain differentiation stages.
Yagita
H
H
Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan.
Nakamura
T
T
Asakawa
J
J
Matsuda
H
H
Tansyo
S
S
Iigo
Y
Y
Okumura
K
K
D000818:Animals; D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D018801:CD2 Antigens; D002454:Cell Differentiation; D002460:Cell Line; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology; D051379:Mice; D011971:Receptors, Immunologic
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D018801:CD2 Antigens; D011971:Receptors, Immunologic