Molecular scanning of Yq11 (interval 6) in men with Sertoli-cell-only syndrome.
Data suggesting that probes pDP105/B and 50f2/C,E may identify sequences on distal Yq11 (interval 6) that are critical for spermatogenesis stimulated a study of this region by means of these two probes in azoospermic 46,XY men with biopsy-proved Sertoli-cell-only syndrome. Deoxyribonucleic acid samples from controls and study subjects were digested with the restriction enzymes TaqI, EcoRI, and BamHI. These samples were blotted and hybridized with pDP105/B, 50f2/C,E, and two more proximal Yq11 probes 4B-2 and pAS1. The sequence hydridizing to 50f2/C was absent in one study subject. No deletions were detected with pDP105/B and the two more proximal probes.
Johnson
M D
MD
Department of Obstetrics and Gynecology, Medical College of Georgia, Augusta 30912-3360.
Tho
S P
SP
Behzadian
A
A
McDonough
P G
PG
D000328:Adult; D004247:DNA / Q000032:analysis / Q000235:genetics; D006801:Humans; D008297:Male; D015335:Molecular Probes; D009693:Nucleic Acid Hybridization; D009845:Oligospermia / Q000473:pathology*; D012708:Sertoli Cells / Q000473:pathology* / Q000648:ultrastructure; D012729:Sex Chromosome Aberrations / Q000473:pathology*; D013091:Spermatogenesis; D013577:Syndrome; D014998:Y Chromosome / Q000473:pathology / Q000648:ultrastructure*
D015335:Molecular Probes; D004247:DNA