title: Cation and anion transport pathways in volume regulatory response of human lymphocytes to hyposmotic media.
abstract: The regulatory volume decrease of osmotically swollen human peripheral blood lymphocytes can be inhibited by agents acting on volume-activated K+- or Cl--transport pathways. Quinine, cetiedil, and 3,3'-dipropylthiadicarbocyanine were found to block the volume-induced K+ transport by interaction with sites on the outside face of the membrane, perhaps by competition with external K+. Drugs known to influence calmodulin action inhibit both volume-induced K+ and Cl- transport to varying degrees. Those inhibitors, particularly of K+ transport, are correlated with their calmodulin-antagonist activity. Penetrating sulfhydryl (SH) reagents (in contrast to nonpenetrating ones) are potent inhibitors of both volume-induced K+ and Cl- movements, indicating the presence of functionally important SH groups located within the membrane or at the cytoplasmic face. A number of agents, such as dipyridamole and oligomycin C, are specific inhibitors of the volume-activated anion pathway. In all respects studied, the inhibition characteristics of the volume-activated K+ pathway of lymphocytes resemble those of the Ca2+-activated K+ channel of red cells. In contrast, the volume-induced anion permeability differs from the primary anion-transport pathway of red cells.
authors.forename: B
authors.initials: B
authors.lastname: Sarkadi
authors.forename: R
authors.initials: R
authors.lastname: Cheung
authors.forename: E
authors.initials: E
authors.lastname: Mack
authors.forename: S
authors.initials: S
authors.lastname: Grinstein
authors.forename: E W
authors.initials: EW
authors.lastname: Gelfand
authors.forename: A
authors.initials: A
authors.lastname: Rothstein
mesh_terms: D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000031:analogs & derivatives*; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D002712:Chlorides / Q000378:metabolism*; D002729:Chloromercuribenzoates / Q000494:pharmacology; D003470:Culture Media; D005033:Ethylmaleimide / Q000494:pharmacology; D006096:Gramicidin / Q000494:pharmacology; D006801:Humans; D008214:Lymphocytes / Q000166:cytology*; D009994:Osmolar Concentration; D011188:Potassium / Q000378:metabolism*; D011803:Quinine / Q000494:pharmacology; D012413:Rubidium / Q000378:metabolism; D020245:p-Chloromercuribenzoic Acid
chemical_list: D002712:Chlorides; D002729:Chloromercuribenzoates; D003470:Culture Media; D006096:Gramicidin; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D020245:p-Chloromercuribenzoic Acid; C018564:dihydro-DIDS; D011803:Quinine; D012413:Rubidium; D005033:Ethylmaleimide; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D011188:Potassium