[The effect of the laser microirradiation of the cell center on neutrophil motility].
The cell center of human neutrophils spread on polylysine-coated coverslips was irradiated with an argon laser microbeam. After the cells were pretreated with acridine orange, the irradiation of the cell center in a dose of over 0.1 J completely and irreversibly suppressed the motility of neutrophils (both random migration and chemotaxis), even though the cells retained their polarization. The same dose, applied to the cell nucleus and the forward and backward edges of the cytoplasm, resulted in little, if any, effect on cell motility, and did not inhibit their movement toward the target. Electron microscopy of the cells with the irradiated center showed the microtubules to persist for no less than 30 minutes; no visible destruction was caused in the cell center structure. Consequently, the cell center directly controls (not through polymerization of microtubules) the motility of neutrophils.
Uzbekov
R E
RE
Vorob'ev
I A
IA
Drachev
V A
VA
D000165:Acridine Orange; D002465:Cell Movement / Q000528:radiation effects; D002469:Cell Separation / Q000379:methods; D002634:Chemotaxis, Leukocyte / Q000528:radiation effects; D003593:Cytoplasm / Q000528:radiation effects* / Q000648:ultrastructure; D006801:Humans; D007834:Lasers; D008854:Microscopy, Electron; D008870:Microtubules / Q000528:radiation effects / Q000648:ultrastructure; D009504:Neutrophils / Q000528:radiation effects* / Q000648:ultrastructure; D013997:Time Factors
D000165:Acridine Orange
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