Autolysis of Caulobacter crescentus grown in the presence of glycine.
Caulobacter crescentus was grown in complex medium supplemented with low (0.05%) concentration of glycine, a component of the murein peptide side chains of this bacterium. Murein synthesized in the presence of glycine was poorly crosslinked and the rate of its synthesis was slowed down compared to the control cells. The glycine-grown cells were considerably more sensitive to the chelating agent EDTA and Tris buffer than the control cells and also lysed faster when incubated with beta-lactam antibiotics. No changes in phospholipid composition in the presence of glycine were observed and the outer membrane protein composition of the glycine-grown cells was altered only in the amount of 130 000 protein which forms the surface array of C. crescentus. The effects of glycine can thus be tentatively put down to the reduced crosslinkage of murein synthesized in its presence.
Markiewicz
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Kwiatkowski
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D000409:Alanine / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects*; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis; D001433:Bacteriolysis / Q000187:drug effects*; D003470:Culture Media; D004492:Edetic Acid / Q000494:pharmacology; D005998:Glycine / Q000494:pharmacology*; D007769:Lactams; D009113:Muramidase / Q000494:pharmacology; D010457:Peptidoglycan / Q000096:biosynthesis; D014325:Tromethamine / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D001425:Bacterial Outer Membrane Proteins; D003470:Culture Media; D007769:Lactams; D010457:Peptidoglycan; D014325:Tromethamine; D004492:Edetic Acid; D009113:Muramidase; D000409:Alanine; D005998:Glycine