Chemical synthesis of peptide fragments of the hormone-specific beta-subunit of human follicle-stimulating hormone.
In order to determine the specific antigenic determinants of human follicle-stimulating hormone (hFSH), hFSH-beta peptides with amino acid residues 33-49 (V2), 95-118 (V3), 76-118 (V3 + 1/2 C2), 1-33 (V1 + C1), 22-33 (1/2C1), and 95-107 (V3 + 1/4C2) according to the nomenclature of Stewart and Stewart [Stewart, M., & Stewart, F. (1977) J. Mol. Biol. 116, 175] as well as additional peptides with the residues 93-107, 91-107, 89-107, 87-107, and 85-107 were chemically synthesized. The peptides were examined in radioimmunoassay systems of FSH, luteinizing hormone (LH), or human chorionic gonadotropin (hCG). V3 + 1/2C2 and V1 + C1 showed immunological activity, whereas the other peptides did not. Antibodies were raised in rabbits against these peptides and examined for specific binding with hFSH, LH, thyroid-stimulating hormone (TSH), and hCG. V3 + 1/2C2 as well as V1 + C1 produced antisera, which specifically bound hFSH, hLH, and hTSH, indicating that the amino acid sequences contained in hFSH-beta peptides V3 + 1/2C2 and V1 + C1 share common antigenic sites with hLH and hTSH. Antisera were produced in rabbits against hFSH-beta, against reduced and S-aminoethylated hFSH-beta (AE-FSH-beta), and against AE-FSH-beta coupled to hemocyanin. Reduced and S-aminoethylated beta-subunit of FSH-beta coupled with hemocyanin produced antisera in rabbits that specifically bound only hFSH and not hLH, hTSH, or hCG.
Saxena
B B
BB
Rathnam
P
P
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D000939:Epitopes / Q000032:analysis; D005640:Follicle Stimulating Hormone / Q000138:chemical synthesis* / Q000276:immunology / Q000302:isolation & purification; D037201:Follicle Stimulating Hormone, beta Subunit; D006801:Humans; D007106:Immune Sera; D007202:Indicators and Reagents; D046911:Macromolecular Substances; D010446:Peptide Fragments / Q000138:chemical synthesis* / Q000302:isolation & purification
D000939:Epitopes; D037201:Follicle Stimulating Hormone, beta Subunit; D007106:Immune Sera; D007202:Indicators and Reagents; D046911:Macromolecular Substances; D010446:Peptide Fragments; D005640:Follicle Stimulating Hormone