Distribution and molecular forms of glucagon-like peptide in the dog.
Using glucagon-like peptide-1 N-terminus and C-terminus directed antisera, we investigated concentration and molecular forms of GLP-1 immunoreactivity (IR) in extracts of various tissues of the dog. GLP-1 IR measured with C-terminus-directed antiserum R2337 (GLP-1 IR-CT) was high in the ileum, appendix, jejunum, colon, and gastric fundus and body. GLP-1 IR measured with N-terminus-directed antiserum R1043 (GLP-1 IR-NT) was high only in the pancreas, and gastric fundus and body. Only GLP-1 IR-CT was found in the hypothalamus, thalamus and medulla oblongata. No immunoreactive materials were detected in the liver, spleen and kidney. Gel-filtration with Sephadex G-50 showed two peaks of both GLP-1 IR-CT and GLP-1 IR-NT, at 10kd and at the position of GLP-1 (1-36 amide) in the pancreatic extract, and one peak at 10kd in the stomach extract. Ileal extracts showed 3 peaks of GLP-1 IR-CT at 10kd, at the position of GLP-1(1-36 amide) and GLP-1(7-36 amide), respectively, but GLP-1 IR-NT was coeluted with GLP-1(1-36 amide). Hypothalamic extracts showed a single peak at the position of GLP-1(7-36 amide). These results suggest that processing of preproglucagon differs in different organs, and that the main GLP-1-related products are a large molecular form and GLP-1(1-36 amide) or GLP-1(1-37) in the pancreas, and GLP-1(7-36 amide) or GLP-1 (7-37) in the ileum and hypothalamus.
Suda
K
K
Third Department of Internal Medicine, Yamagata University School of Medicine, Japan.
Takahashi
H
H
Fukase
N
N
Manaka
H
H
Tominaga
M
M
Sasaki
H
H
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D001923:Brain Chemistry; D002850:Chromatography, Gel; D004064:Digestive System / Q000032:analysis; D004285:Dogs; D005934:Glucagon / Q000032:analysis* / Q000276:immunology; D052216:Glucagon-Like Peptide 1; D007031:Hypothalamus / Q000032:analysis; D007082:Ileum / Q000032:analysis; D010179:Pancreas / Q000032:analysis; D010446:Peptide Fragments / Q000032:analysis* / Q000276:immunology; D011498:Protein Precursors / Q000032:analysis* / Q000276:immunology; D011863:Radioimmunoassay; D013270:Stomach / Q000032:analysis; D014018:Tissue Distribution
D000906:Antibodies; D010446:Peptide Fragments; D011498:Protein Precursors; D052216:Glucagon-Like Peptide 1; D005934:Glucagon