Characterization of cloned human alphoid satellite with an unusual monomeric construction: evidence for enrichment in HeLa small polydisperse circular DNA.
A recombinant DNA plasmid library was constructed from HeLa cell extrachromosomal circular DNA and the sequence organization of one family of clones, which contain sequences enriched in HeLa small polydisperse circular (spc) DNA, was studied by restriction mapping and base sequence analysis. Restriction mapping revealed each clone to be composed solely of imperfect tandem repeats of ca. 170 bp. The entire DNA sequence of one clone was determined and found to be alphoid satellite with a variant monomeric construction.
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D001483:Base Sequence; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004270:DNA, Circular / Q000032:analysis* / Q000302:isolation & purification; D004274:DNA, Recombinant / Q000032:analysis*; D004276:DNA, Satellite / Q000032:analysis*; D006367:HeLa Cells / Q000032:analysis*; D006801:Humans; D012313:RNA / Q000032:analysis; D012091:Repetitive Sequences, Nucleic Acid
D004270:DNA, Circular; D004274:DNA, Recombinant; D004276:DNA, Satellite; D012313:RNA; D004262:DNA Restriction Enzymes