Inhibition of RNA nucleo-cytoplasmic translocation by anti-nucleus antibody.
Anti-nucleus antibody was raised by immunizing rabbits with rat liver nuclei, and purified by affinity-column chromatography. When purified anti-nucleus IgG molecules were introduced into FL cells by the erythrocyte-ghost fusion method with HVJ (Sendai virus), release of RNA from the nucleus into the cytoplasm was inhibited in the presence of alpha-amanitin, but nuclear accumulation of 125I-labeled non-histone chromosomal protein from the cytoplasm was not inhibited. These findings suggest that the nucleo-cytoplasmic transport mechanisms of RNA and nuclear proteins are different. The molecular weight of the antigen of this antibody was determined to be about 55K by the immunoblotting technique.
Sugawa
H
H
Uchida
T
T
D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport; D002460:Cell Line; D002467:Cell Nucleus / Q000276:immunology* / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000502:physiology; D006801:Humans; D007074:Immunoglobulin G / Q000502:physiology*; D008099:Liver / Q000648:ultrastructure; D008561:Membrane Fusion; D010222:Parainfluenza Virus 1, Human; D012313:RNA / Q000378:metabolism*; D051381:Rats
D000546:Amanitins; D002868:Chromosomal Proteins, Non-Histone; D007074:Immunoglobulin G; D012313:RNA