Immunologic tolerance to DNA in B cell lines from both normal and autoimmune mice.
We examine whether B cell lines enriched for DNA specificity from either autoimmune (BWF1) or normal mice (Balb/c) can be rendered unresponsive to autoantigen in terms of the specific suppression of direct antibody-forming cells to DNA. These B cell lines were both Lyt-1 positive and negative. Preincubation with oligonucleotide, covalently linked to mouse gamma-globulin, specifically suppressed the antigen-driven response elicited by DNA horse red blood cells in B cell lines from both strains of mice. There is a 5-fold difference in susceptibility to DNA-specific tolerance induction between B cell lines of BWF1 and Balb/c mice. Thus, B cells from autoimmune mice do not appear to have an inherent absolute defect in being rendered tolerant to autoantigen, but are relatively less susceptible to DNA-specific tolerance than nonautoimmune cell lines.
Aldo-Benson
M
M
Department of Medicine, Indiana University School of Medicine, Indianapolis.
Borel
H
H
Scheiderer-Pratt
L
L
Borel
Y
Y
D000818:Animals; D001324:Autoantigens / Q000008:administration & dosage; D015551:Autoimmunity; D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D004247:DNA / Q000276:immunology*; D007108:Immune Tolerance; D051379:Mice
D001324:Autoantigens; D004247:DNA