Trophic effects of cholecystokinin and calcitonin gene-related peptide on ventral spinal cord in culture.
We studied trophic effects of cholecystokinin (CCK) and calcitonin gene-related peptide (CGRP) on explanted cultures of ventral spinal cord from 13-14 old day rat embryo. There was a significant neurite promoting effect (NPE) in CCK-treated culture. The neuritic extension of ventral spinal cord exceeds control values 3.5-6.5 times. Otherwise, CGRP and NPE at any concentration. The possible mechanism of trophic effect of CCK on ventral spinal cord was briefly discussed. Our results may contribute to a therapeutic strategy for amyotrophic lateral sclerosis.
Iwasaki
Y
Y
Fourth Department of Internal Medicine Toho University Ohashi Hospital, Tokyo, Japan.
Kinoshita
M
M
Ikeda
K
K
Shiojima
T
T
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D002766:Cholecystokinin / Q000494:pharmacology*; D003712:Dendrites / Q000187:drug effects / Q000502:physiology*; D004622:Embryo, Mammalian; D009414:Nerve Growth Factors / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000166:cytology* / Q000187:drug effects
D009414:Nerve Growth Factors; D002766:Cholecystokinin; D015740:Calcitonin Gene-Related Peptide