title: Simultaneous determination of (R)- and (S)-celiprolol in human plasma and urine: high-performance liquid chromatographic assay on a chiral stationary phase with fluorimetric detection.
abstract: The quantitative enantiospecific determination of the beta 1-selective adrenergic antagonist (R,S)-celiprolol in human plasma and urine is described. It involves a two-step liquid-liquid extraction of celiprolol from biological material and separation of the underivatized enantiomers by high-performance liquid chromatography on a chiral stationary phase (cellulose tris-3,5-dimethylphenyl carbamate, coated on silica gel) with fluorimetric detection. R-(+)-Propranolol was used as an internal standard. The detection limits of 1.5 ng/ml enantiomer in plasma and 2.5 ng/ml enantiomer in urine at signal-to-noise ratios higher than 3 permit the performance of pharmacokinetic studies after therapeutic doses.
authors.affiliation: Pharmakologisches Institut für Naturwissenschaftler, Johann Wolfgang Goethe-Universität, Frankfurt am Main, F.R.G.
authors.forename: C
authors.initials: C
authors.lastname: Hartmann
authors.forename: D
authors.initials: D
authors.lastname: Krauss
authors.forename: H
authors.initials: H
authors.lastname: Spahn
authors.forename: E
authors.initials: E
authors.lastname: Mutschler
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D017272:Celiprolol; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D011412:Propanolamines / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism
chemical_list: D000319:Adrenergic beta-Antagonists; D011412:Propanolamines; D017272:Celiprolol