title: Characterization of adenosine-bovine serum albumin immunogen and its antibodies.
abstract: Antibodies to adenosine were elicited in rabbits using a conjugate of adenosine (periodate oxidized) and bovine serum albumin as immunogen. The conjugate was found to be of a higher molecular size than the original protein, apparently due to the cross-linking of albumin molecules by the dialdehydes generated during the periodate oxidation of adenosine. It produced high titres of antibodies, which were 400-fold more reactive to adenosine-trialcohol than to adenosine, when measured by a nitrocellulose filter assay. In addition, a specific elution procedure for the isolation of hapten-specific antibodies is described.
authors.forename: M A
authors.initials: MA
authors.lastname: Ali
authors.forename: T M
authors.initials: TM
authors.lastname: Jacob
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D000918:Antibody Specificity; D002846:Chromatography, Affinity; D000939:Epitopes; D006241:Haptens; D008970:Molecular Weight; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000276:immunology; D013329:Structure-Activity Relationship
chemical_list: D000906:Antibodies; D000939:Epitopes; D006241:Haptens; D012710:Serum Albumin, Bovine; D000241:Adenosine