title: The fate of lipopolysaccharide in cultured rat Kupffer cells.
abstract: Cultured rat Kupffer cells were incubated in presence of biologically tritiated Salmonella abortus equi lipopolysaccharide. Uptake of lipopolysaccharide increased rapidly during the first 2 h of incubation and then levelled off. Within the first h of incubation 10(6) Kupffer cells were able to ingest up to 18 micrograms lipopolysaccharide. Kupffer cells metabolised lipopolysaccharide and released lipopolysaccharide-related substances, but neither the cell-associated lipopolysaccharide nor the released lipopolysaccharide products were detoxified, as measured by the mouse lethality test.
authors.affiliation: Laboratory for Cell Biology and Histology, Free University Laarbeeklaan, Brussels-Jette, Belgium.
authors.forename: H
authors.initials: H
authors.lastname: Van Bossuyt
authors.forename: C
authors.initials: C
authors.lastname: Desmaretz
authors.forename: E
authors.initials: E
authors.lastname: Wisse
mesh_terms: D000818:Animals; D001693:Biological Transport, Active; D002478:Cells, Cultured; D007700:Kinetics; D007728:Kupffer Cells / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000378:metabolism / Q000493:pharmacokinetics* / Q000633:toxicity; D051379:Mice; D008810:Mice, Inbred C57BL; D051381:Rats
chemical_list: D008070:Lipopolysaccharides