title: Quantitative selection of denitrifying bacteria in continuous cultures and requirement for organic carbon. I. Starch.
abstract: A mixed population of bacteria from bottom sludge of nitrogen wastewater reservoir was incubated in continuous culture in medium containing 1000 mg nitrate nitrogen/l and starch. Maximal efficiency of denitrification was 5 mg N/l/h. Marked changes in participation of denitrifying bacteria (16-76%) among total number of bacteria was observed, this being dependent on the ratio of starch concentration (CS) to nitrogen concentration (CN) in the medium. The optimal CS/CN ratio ensuring highest participation of denitrifying bacteria was 3.2. The amount of starch required for the denitrification of a defined quantity of nitrogen is negatively correlated (r = -0.98) with the frequency of the occurrence of denitrifying bacteria (XD) and is: CS = (5.53-0.028XD) CN. The denitrifying bacteria in continuous culture were dominated, depending on CS/CN ratio and flow rate of medium, by Alcaligenes faecalis, Paracoccus denitrificans or Pseudomonas mendocina, that is species unable to hydrolyse starch.
authors.forename: R
authors.initials: R
authors.lastname: Mycielski
authors.forename: H
authors.initials: H
authors.lastname: Jaworowska-Deptuch
authors.forename: M
authors.initials: M
authors.lastname: Błaszczyk
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D003470:Culture Media; D009566:Nitrates / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism*; D013213:Starch / Q000494:pharmacology*
chemical_list: D003470:Culture Media; D009566:Nitrates; D002244:Carbon; D013213:Starch; D009584:Nitrogen