A DQA1 allele is strongly associated with idiopathic membranous nephropathy.
The high molecular weight genomic DNA from 31 patients with idiopathic membranous nephropathy (IMN) has been digested with a restriction endonuclease Taq I, electrophoresed, blotted and hybridised with probes to the HLA-DRB, -DQA, -DQB, -DPA and -DPB genes. The restriction endonuclease Msp I was also used with the HLA-DPA and -DPB probes. The resulting restriction fragment length polymorphism (RFLP) has been analysed and compared with 55 controls treated in the same way. There was a significant increase in the DRB restriction fragments associated with HLA-DR3 (Fisher's p = 0.011), in particular with a sub-division of DR3 (Fisher's p = 0.0038). These results confirm at the DNA level serological correlations observed for IMN. A 4.5 Kb DQA RFLP was significantly raised in IMN patients (Fisher's p = 0.002) and is proposed as a major disease susceptibility factor.
Vaughan
R W
RW
Department of Molecular Immunogenetics, Guy's Hospital, London, UK.
Demaine
A G
AG
Welsh
K I
KI
D000483:Alleles; D015139:Blotting, Southern; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D015342:DNA Probes; D015433:Glomerulonephritis, Membranous / Q000235:genetics* / Q000276:immunology; D006682:HLA-DP Antigens / Q000235:genetics; D006683:HLA-DQ Antigens / Q000235:genetics; D015803:HLA-DR3 Antigen / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length; D044465:White People / Q000235:genetics
D015342:DNA Probes; D006682:HLA-DP Antigens; D006683:HLA-DQ Antigens; D015803:HLA-DR3 Antigen; D004247:DNA