title: Corticotropin-releasing factor-binding protein is a glycoprotein.
abstract: Human corticotropin-releasing factor-binding protein (hCRF-BP), a 38,000 dalton protein, specifically binds hCRF in plasma. CRF-BP-CRF complex adsorbed to concanavalin-A-Sepharose and its Mr decreased after treatment with endoglycosidase H or glycopeptidase A. The binding of CRF-BP to CRF decreased after treatment with endoglycosidase H. These results indicate that the CRF-BP is a glycoprotein that contains asparagine N-linked-type oligosaccharides, and such oligosaccharide chains are important for CRF-BP binding.
authors.affiliation: Department of Medicine, Tokyo Women's Medical College, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Suda
authors.forename: T
authors.initials: T
authors.lastname: Sumitomo
authors.forename: F
authors.initials: F
authors.lastname: Tozawa
authors.forename: T
authors.initials: T
authors.lastname: Ushiyama
authors.forename: H
authors.initials: H
authors.lastname: Demura
mesh_terms: D002352:Carrier Proteins / Q000097:blood* / Q000302:isolation & purification; D002846:Chromatography, Affinity; D003346:Corticotropin-Releasing Hormone / Q000097:blood*; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000097:blood*; D006026:Glycoside Hydrolases; D006801:Humans; D008970:Molecular Weight; D012016:Reference Values
chemical_list: D002352:Carrier Proteins; D006023:Glycoproteins; D003346:Corticotropin-Releasing Hormone; D006026:Glycoside Hydrolases