Electroporation induced transformation of Bacteroides ruminicola and Bacteroides uniformis by plasmid DNA.
Frequencies of greater than 10(5) transformants per microgram DNA were achieved in Bacteroides ruminicola F101 by electroporation of cells under anaerobic conditions, using the 19.5 kbp tetracycline resistance plasmid pRRI4. Similar procedures gave frequences of 10(6) erythromycin resistant transformants per microgram DNA with the shuttle plasmid pDP1 (19 kbp) in Bacteroides uniformis. Transformation of B. uniformis occurred at a far lower frequency (10(3) micrograms) when pDP1 DNA was derived from E. coli rather than B. uniformis.
Thomson
A M
AM
Rowett Research Institute, Bucksburn, Aberdeen, U.K.
Flint
H J
HJ
D001439:Bacteroides / Q000235:genetics* / Q000254:growth & development; D004269:DNA, Bacterial / Q000235:genetics*; D004563:Electrochemistry; D010957:Plasmids; D014169:Transformation, Bacterial
D004269:DNA, Bacterial