Effect of haem arginate on colony formation by haematopoietic progenitors in vitro.
The effect of haem arginate, a new stable haem compound, was studied on colony formation by erythroid (CFU-E, BFU-E) and granulocyte-macrophage (CFU-GM) progenitors from the bone marrow of 12 healthy persons. At the concentration of 10 mumol/l haem arginate significantly (p less than 0.05) increased and at the concentration of 200 mumol/l significantly decreased (p less than 0.005) the colony formation of CFU-E. A similar, though not significant, trend was seen in the BFU-E colony growth, while the CFU-GM colony formation was not influenced at any concentration between 1 and 200 mumol/l. We conclude that the stimulation of erythropoiesis in vitro is seen at lower concentrations with haem arginate than with former haemin preparations.
Partanen
S
S
Third Department of Medicine, Helsinki University Central Hospital, Finland.
Juvonen
E
E
Tenhunen
R
R
Ruutu
T
T
D001120:Arginine / Q000494:pharmacology*; D003114:Colony-Forming Units Assay; D006418:Heme / Q000494:pharmacology*; D006801:Humans; D008222:Lymphokines / Q000276:immunology*; D019714:Tissue Inhibitor of Metalloproteinases
D008222:Lymphokines; D019714:Tissue Inhibitor of Metalloproteinases; D006418:Heme; D001120:Arginine; C048849:heme arginate