title: Influence of water-immersion stress on synthesis of mucus glycoprotein in the rat gastric mucosa.
abstract: Gastric mucous cells of rats subjected to water-immersion stress were incubated with [3H]-palmitic acid and [14C]-N-acetylgalactosamine. The peptidyl-tRNA released by gastric polysomes was precipitated with cold ethanol and then the content was determined. A 70% reduction in the peptidyl-tRNA isolated was observed in the stressed rats, as compared with in control rats. The peptide recovered from the peptidyl-tRNA showed 30-50% less [3H]-palmitic acid and [14C]-N-acetylgalactosamine incorporation in the stressed rats than in normal controls. It was thus suggested that translation, acylation and glycosylation of the peptides in the ribosomes of the gastric mucosa were remarkably affected by the stress.
authors.affiliation: Division of Metabolism and Clinical Nutrition, Department of Internal Medicine, Kyoto University School of Medicine, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Tsukada
authors.forename: Y
authors.initials: Y
authors.lastname: Seino
authors.forename: S
authors.initials: S
authors.lastname: Ueda
authors.forename: H
authors.initials: H
authors.lastname: Uchino
authors.forename: M
authors.initials: M
authors.lastname: Sakai
mesh_terms: D000116:Acetylgalactosamine / Q000378:metabolism; D000818:Animals; D005753:Gastric Mucosa / Q000378:metabolism*; D006023:Glycoproteins / Q000096:biosynthesis*; D007101:Immersion; D008297:Male; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D012343:RNA, Transfer; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013312:Stress, Physiological / Q000209:etiology / Q000378:metabolism*
chemical_list: D006023:Glycoproteins; D010169:Palmitic Acids; D012346:RNA, Transfer, Amino Acyl; C047874:gastric mucus glycoproteins; C019037:tRNA, peptidyl-; D019308:Palmitic Acid; D012343:RNA, Transfer; D000116:Acetylgalactosamine