title: Conformational changes induced by Mg2+ on actin monomers. An immunologic attempt to localize the affected region.
abstract: The effect of Mg2+ ions on the conformation of G-actin and in particular on the accessibility of its antigenic regions has been tested. Experiments were performed with G-actin coupled to Sepharose 4B which was, therefore, maintained in the monomeric state. The results presented her show that the 2mM MgCl2-perturbed antigenic site is located in a central region of the actin sequence.
authors.forename: C
authors.initials: C
authors.lastname: Roustan
authors.forename: Y
authors.initials: Y
authors.lastname: Benyamin
authors.forename: M
authors.initials: M
authors.lastname: Boyer
authors.forename: R
authors.initials: R
authors.lastname: Bertrand
authors.forename: E
authors.initials: E
authors.lastname: Audemard
authors.forename: J
authors.initials: J
authors.lastname: Jauregui-Adell
mesh_terms: D000199:Actins / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D010450:Endopeptidases / Q000378:metabolism; D000939:Epitopes / Q000032:analysis*; D008274:Magnesium / Q000494:pharmacology*; D009132:Muscles / Q000032:analysis; D009282:Naphthalenesulfonates; D010446:Peptide Fragments / Q000032:analysis; D011487:Protein Conformation / Q000187:drug effects; D011817:Rabbits; D012697:Serine Endopeptidases; D012967:Sodium Dodecyl Sulfate
chemical_list: D000199:Actins; D000939:Epitopes; D009282:Naphthalenesulfonates; D010446:Peptide Fragments; D012967:Sodium Dodecyl Sulfate; C006421:1,5-I-AEDANS; D010450:Endopeptidases; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase; D008274:Magnesium