Measurement of IgG in equine blood by immunoturbidimetry and latex agglutination.
This paper describes the quantitative measurement of IgG concentrations in equine blood/serum by turbidimetric analysis and the qualitative assessment using latex agglutination. The concentration of IgG in foal serum measured by turbidimetry correlated well with the results obtained by radial immunodiffusion (r = 0.91) and the gamma-globulins estimated from the electrophoresis of serum on cellulose acetate (r = 0.92). The method, using antibody-coated latex beads, to detect foals with serum IgG concentrations of less than 4 g/litre (whole blood less than 2 g/litre) proved to be accurate in 96 per cent of cases and provided a quick and simple qualitative test, suitable for use outside the laboratory.
Kent
J E
JE
Blackmore
D J
DJ
D000818:Animals; D006736:Horses / Q000276:immunology*; D007074:Immunoglobulin G / Q000378:metabolism*; D007841:Latex Fixation Tests; D009391:Nephelometry and Turbidimetry; D005719:gamma-Globulins / Q000378:metabolism
D007074:Immunoglobulin G; D005719:gamma-Globulins