Comparative biochemistry of hepatic arylsulfatases from north and south American opossums.
1. The Virginia opossum (Didelphis virginiana) possessed an arylsulfatase which had a relative molecular weight of 130 +/- 12 kDa, displayed anomalous kinetics, hydrolysed AA2S, and exhibited other properties of arylsulfatase A. No arylsulfatase B was found. 2. The arylsulfatase present in the gray short-tailed opossum (Monodelphis domestica) had a relative molecular weight of 56 +/- 4 kDa, exhibited linear kinetics, was inhibited by chloride, and possessed other characteristics of arylsulfatase B. No arylsulfatase A was found. 3. Arylsulfatases from both species occurred as multiple isozymes which were unaffected by neuraminidase or alkaline phosphatase treatment.
Daniel
W L
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Department of Cell and Structural Biology, University of Illinois, Urbana 61801.
D000818:Animals; D001192:Arylsulfatases / Q000378:metabolism*; D002553:Cerebroside-Sulfatase / Q000378:metabolism; D002712:Chlorides / Q000494:pharmacology; D002803:Chondro-4-Sulfatase / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D007527:Isoenzymes; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D009656:North America; D009893:Opossums / Q000378:metabolism*; D013020:South America; D013429:Sulfatases / Q000378:metabolism*
D002712:Chlorides; D007527:Isoenzymes; D013429:Sulfatases; D001192:Arylsulfatases; D002553:Cerebroside-Sulfatase; D002803:Chondro-4-Sulfatase