title: Isolation of turbot (Scophthalmus maximus) vitellogenin by high-performance anion-exchange chromatography.
abstract: The use of high-performance anion-exchange chromatography on a Mono Q column for isolation of a glycolipophosphoprotein, vitellogenin, from turbot plasma has been evaluated. The method is an effective, rapid one-step procedure, which gives a pure preparation of vitellogenin as assessed by electrophoresis, [32P]orthophosphate incorporation and amino acid composition.
authors.affiliation: Department of Zoophysiology, University of Göteborg, Sweden.
authors.forename: C
authors.initials: C
authors.lastname: Silversand
authors.forename: C
authors.initials: C
authors.lastname: Haux
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001345:Autoradiography; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005412:Flatfishes; D010710:Phosphates / Q000378:metabolism; D014819:Vitellogenins / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D000596:Amino Acids; D010710:Phosphates; D014819:Vitellogenins