title: Voltage-dependent removal of sodium inactivation by N-bromoacetamide and pronase.
abstract: When perfused internally through crayfish giant axons, pronase removed sodium inactivation more than three times as fast at -100 mV as compared with -30 mV. N-bromoacetamide, applied internally, removed sodium inactivation twice as fast at -100 mV as at -30 mV, and the relative rate of removal declined with membrane depolarization in proportion to steady-state sodium inactivation. We conclude that in the closed conformation the sodium inactivation gate is partially protected from destruction by N-bromoacetamide and pronase.
authors.forename: V L
authors.initials: VL
authors.lastname: Salgado
authors.forename: J Z
authors.initials: JZ
authors.lastname: Yeh
authors.forename: T
authors.initials: T
authors.lastname: Narahashi
mesh_terms: D000081:Acetamides / Q000494:pharmacology*; D000818:Animals; D003400:Astacoidea; D001369:Axons / Q000502:physiology*; D004558:Electric Stimulation; D007473:Ion Channels / Q000187:drug effects* / Q000502:physiology; D008564:Membrane Potentials; D011402:Pronase / Q000494:pharmacology*; D012964:Sodium / Q000378:metabolism*
chemical_list: D000081:Acetamides; D007473:Ion Channels; C016272:N-bromoacetamide; D012964:Sodium; D011402:Pronase