Peculiar features of application of pH gradients formed in borate buffer with a polyhydroxy compound for separation of proteins in a free-flow electrophoretic apparatus.
The use of pH gradients in borate buffer with a polyhydroxy compound for separation of proteins in a free-flow electrophoretic apparatus is outlined. The composition of the pH gradients, the free-flow apparatus design and its operation, and the main causes of failures in the protein separation are considered.
Shukun
S A
SA
Institute of Immunology, Ministry of Medical and Microbiological Industry, Lyubuchany, Moscow Region, Chekhov District, U.S.S.R.
Zav'yalov
V P
VP
D001881:Borates; D002021:Buffers; D004586:Electrophoresis / Q000295:instrumentation*; D006863:Hydrogen-Ion Concentration; D011506:Proteins / Q000302:isolation & purification*
D001881:Borates; D002021:Buffers; D011506:Proteins