title: Site-specific alteration of Gly-24 in streptokinase: its effect on plasminogen activation.
abstract: Oligonucleotide-directed mutagenesis was carried out to replace glycine-24 of streptokinase with histidine, glutamic acid, or alanine. Substitutions with either histidine or glutamic acid resulted in almost complete loss of streptokinase activity but streptokinase replaced with alanine retained its activity. Although streptokinases with histidine-24 or glutamic acid-24 bound normally to human plasminogen, they were not able to generate active plasmin, whereas those with alanine-24 or glycine-24 (wild-type) could generate active plasmin. The results indicate that the small, uncharged alkyl group side-chain on the 24th amino acid residue of streptokinase is indispensable for the activity of the human plasminogen-streptokinase complex.
authors.affiliation: Department of Biological Science and Engineering, Korea Advanced Institute of Science and Technology, Seoul.
authors.forename: B R
authors.initials: BR
authors.lastname: Lee
authors.forename: S K
authors.initials: SK
authors.lastname: Park
authors.forename: J H
authors.initials: JH
authors.lastname: Kim
authors.forename: S M
authors.initials: SM
authors.lastname: Byun
mesh_terms: D000409:Alanine; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005971:Glutamates; D018698:Glutamic Acid; D005998:Glycine; D006639:Histidine; D006801:Humans; D008970:Molecular Weight; D009154:Mutation; D010958:Plasminogen / Q000378:metabolism*; D011863:Radioimmunoassay; D013300:Streptokinase / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D013329:Structure-Activity Relationship
chemical_list: D005971:Glutamates; D018698:Glutamic Acid; D006639:Histidine; D010958:Plasminogen; D013300:Streptokinase; D000409:Alanine; D005998:Glycine