Antitumor activity of normal human globulin: effects on murine and human erythroleukemic cells.
The effect of the antitumor fraction isolated from the alpha 2-globulin region of normal human serum (NHG-I) upon murine (FELC) and human (K562) erythroleukemic cells in vitro was determined. NHG-I inhibited the growth of both actively growing FELC and K562 cells in a dose-dependent manner. However, it has no effect upon mitomycin C treated FELC which were unable to divide nor upon dimethylsulfoxide-induced FELC in stationary phase. These results indicate that NHG-I has a cytostatic effect upon cell growth and suggests that its action may be dependent upon DNA synthesis. This is in marked contrast to TNF, the alpha 2-globulin factor obtained from murine serum which is also not species-specific but whose action upon these cell lines is cytotoxic.
Suyama
K
K
Goldstein
J
J
Green
S
S
D000510:Alpha-Globulins / Q000276:immunology* / Q000633:toxicity; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D006023:Glycoproteins / Q000276:immunology*; D006131:Growth Inhibitors / Q000633:toxicity; D006454:Hemoglobins / Q000096:biosynthesis; D007167:Immunotherapy; D004915:Leukemia, Erythroblastic, Acute / Q000628:therapy; D007942:Leukemia, Experimental / Q000628:therapy*; D051379:Mice; D009439:Neuraminidase; D014409:Tumor Necrosis Factor-alpha
D000510:Alpha-Globulins; D006023:Glycoproteins; D006131:Growth Inhibitors; D006454:Hemoglobins; D014409:Tumor Necrosis Factor-alpha; D009439:Neuraminidase; D004121:Dimethyl Sulfoxide