Major structural alterations of the c-sis gene are not observed in a series of tumors of the human central nervous system.
Expression of the c-sis oncogene, the gene encoding the B chain of platelet-derived growth factor (PDGF), may be related to initiation and/or progression of glial cell tumorigenesis by PDGF-mediated autocrine growth stimulation. As the mechanism for activation of expression of the c-sis gene in gliomas is not known, we searched for possible structural alterations of c-sis DNA in these tumors. Genomic Southern blots of DNA from 7 different cultured human glioblastoma cell lines and 15 different solid human brain tumors revealed no significant change in either the gross structure or the copy number of the c-sis gene in tumor cells vs. control cells. Activation of glioma c-sis gene expression is therefore not the result of a gross rearrangement or amplification of the c-sis gene. Expression of c-sis mRNA was detected in all of 12 different solid human brain tumors, 11 of which were of glial cell origin. However, in tissue adjacent to 5 different tumors, approximately the same level of c-sis mRNA was seen.
Press
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Department of Biochemistry, Case Western Reserve University Cleveland, OH 44106.
Misra
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A
Samols
D
D
Goldthwait
D A
DA
Mapstone
T B
TB
D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D002648:Child; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005910:Glioma / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D020575:Oncogene Proteins v-sis; D010982:Platelet-Derived Growth Factor / Q000235:genetics* / Q000378:metabolism; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics*
D004273:DNA, Neoplasm; D020575:Oncogene Proteins v-sis; D010982:Platelet-Derived Growth Factor; D015682:Retroviridae Proteins, Oncogenic