Monoclonal antibodies to mitochondrial coupling factor B.
Two monoclonal antibodies (MAb I and IV) have been prepared which showed high and specific reactions towards bovine heart mitochondrial coupling factor B (FB). Both have been identified as sub-type IgG1 of mouse immunoglobulins. MAb I reacts with purified and functionally active FB, alkylated or oxidized forms of FB and even with peptides formed on digestion of FB with trypsin. When used together, MAb I and IV reacted with FB in immunoblots of normal and urea treated samples of mitochondria, submitochondrial particles, ammonia-EDTA extracted particles, and H+-ATPase. Both MAbs inhibited FB-stimulated ATP-dependent reverse electron flow activity when FB was incubated with the antibody either before or after its addition to FB-deficient AE-particles. Reactivity of MAb I towards FB declined upon exposure of FB to guanidine HC1 while reactivity of MAb IV remained unaltered.
Joshi
S
S
Kantham
L
L
Kaplay
S
S
Sanadi
D R
DR
D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D008929:Mitochondria, Heart / Q000378:metabolism*; D010446:Peptide Fragments / Q000032:analysis; D006180:Proton-Translocating ATPases / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D014357:Trypsin
D000911:Antibodies, Monoclonal; D000939:Epitopes; D010446:Peptide Fragments; D014357:Trypsin; D006180:Proton-Translocating ATPases