The role of interleukin 6 and interleukin 1 in megakaryocyte development.
The effect of human interleukin 6 (IL-6) and interleukin 1 (IL-1) on cells of the megakaryocyte lineage from murine bone marrow was examined. In bone marrow liquid culture, IL-6 but not IL-1 increases the amount of acetylcholinesterase, a megakaryocyte marker. In semisolid colony assays, a low level of interleukin 3 (IL-3) was used as a growth factor, and IL-6 and IL-1 were tested for their ability to potentiate the activity of IL-3 to stimulate megakaryocyte colony formation. IL-6 and/or IL-1 had no effect on megakaryocyte colony formation in the absence of IL-3. However, IL-6 was able to stimulate increased megakaryocyte colonies in the presence of IL-3. IL-1 was able to potentiate colony formation only in the presence of both IL-3 and IL-6.
Warren
M K
MK
Department of Cell Biology, Cetus Corporation, Emeryville, California 94608.
Conroy
L B
LB
Rose
J S
JS
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D006410:Hematopoiesis / Q000187:drug effects*; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000494:pharmacology*; D007377:Interleukin-3 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology*; D008533:Megakaryocytes / Q000201:enzymology / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C
D007375:Interleukin-1; D007377:Interleukin-3; D015850:Interleukin-6; D000110:Acetylcholinesterase