[The mechanism of action of a synthetic derivative of 1,4-naphthoquinone on the respiratory chain of liver and heart mitochondria].
It was found that the 1.4-naphthoquinone derivative AK-135 (2-methyl-3-piperidine-methyl-1.4-naphthoquinone hydrochloride) possesses a marked acceptor capacity during succinate and glutamate oxidation by rat liver and rabbit heart mitochondria. AK-135 fully restores the rate of glutamate (but not succinate) oxidation by liver and heart mitochondria catalyzed by rotenone, antimycin A and cyanide. In non-phosphorylating preparations of liver and heart mitochondria, AK-135 eliminates the inhibition of respiration on exogenous NADH induced by the same electron transport inhibitors. In liver mitochondria, the stimulation of succinate oxidation is due to a reverse electron transfer, whereas in the heart it proceeds via the rotenone-insensitive pathway. The experimental results suggest that in the liver and heart AK-135 accepts electrons from NADH-dehydrogenase oxidizing endogenous NADH. Besides, in the liver this compound is also capable of accepting electrons from NADH-cytochrome b5 reductase.
Levin
G S
GS
Tremasova
G Ia
GIa
Kostova
S V
SV
Dregeris
Ia Ia
IaIa
D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D002384:Catalysis; D003486:Cyanides / Q000494:pharmacology; D015786:Cytochromes b5 / Q000378:metabolism; D004579:Electron Transport; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D008297:Male; D008929:Mitochondria, Heart / Q000187:drug effects* / Q000201:enzymology; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000201:enzymology; D009243:NAD / Q000378:metabolism; D009285:Naphthoquinones / Q000494:pharmacology*; D010084:Oxidation-Reduction; D010101:Oxygen Consumption / Q000187:drug effects*; D011817:Rabbits; D051381:Rats; D012402:Rotenone / Q000494:pharmacology; D013385:Succinate Dehydrogenase / Q000378:metabolism
D003486:Cyanides; D005971:Glutamates; D009285:Naphthoquinones; D012402:Rotenone; D009243:NAD; D018698:Glutamic Acid; D000968:Antimycin A; D015786:Cytochromes b5; D013385:Succinate Dehydrogenase
K mekhanizmu deĭstviia sinteticheskogo proizvodnogo 1,4-naftokhinona na dykhatel'nuiu tsep' mitokhondriĭ pecheni i serdtsa.