Cell-mediated and humoral immune response to Mycoplasma hyopneumoniae in pigs enhanced by dextran sulfate.
The effect of dextran sulfate (DS), known to be cytotoxic to macrophages, on the cell-mediated and humoral immune response to nonviable Mycoplasma hyopneumoniae in pigs was investigated. The cell-mediated immune response was determined by means of lymphocyte transformation a test, using uptake of [3H]thymidine in a microculture system and the humoral immune response by means of a microplate complement-fixation test. Peripheral blood lymphocytes from pigs vaccinated with nonviable M hyopneumoniae and DS incorporated substantially more [3H]thymidine than did those from pigs given Mycoplasma or DS alone. The transformation of lymphocytes from M hyopneumoniae-DS vaccinated pigs was enhanced when M hyopneumoniae cells used in the assay system were heated at 60 C for 30 minutes. Similarly prepared M flocculare and M hyorhinis cells also stimulated lymphocytes from M hyopneumoniae-DS vaccinated pigs, but not nearly as great as when M hyopneumoniae cells were used. The humoral antibody response and the cell-mediated immune response to nonviable M hyopneumoniae was markedly enhanced by DS. Pigs were vaccinated with nonviable M hyopneumoniae and/or DS 4 times and challenge exposed intratracheally with viable M hyopneumoniae. Pigs vaccinated with M hyopneumoniae and DS had less severe pneumonia than did nonvaccinated pigs.
Kishima
M
M
Ross
R F
RF
Kuniyasu
C
C
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D000942:Antigens, Bacterial / Q000276:immunology; D003168:Complement Fixation Tests / Q000662:veterinary; D016264:Dextran Sulfate; D003911:Dextrans / Q000494:pharmacology*; D000939:Epitopes; D006358:Hot Temperature; D007114:Immunization / Q000662:veterinary*; D008168:Lung / Q000473:pathology; D008213:Lymphocyte Activation / Q000187:drug effects*; D009174:Mycoplasma / Q000276:immunology*; D045729:Pneumonia of Swine, Mycoplasmal / Q000473:pathology / Q000662:veterinary; D013552:Swine / Q000276:immunology*; D013553:Swine Diseases / Q000473:pathology
D000942:Antigens, Bacterial; D003911:Dextrans; D000939:Epitopes; D016264:Dextran Sulfate