Transfection of Leishmania enriettii and expression of chloramphenicol acetyltransferase gene.
We report a transient expression transfection system in Leishmania enriettii. A hybrid gene containing an intergenic region of the alpha-tubulin cluster and the bacterial chloramphenicol acetyltransferase (CAT; EC 2.3.1.28) gene is expressed after transfection of L. enriettii with the hybrid plasmid. The expression of the CAT gene is dependent on the presence of sequences from the alpha-tubulin gene. The hybrid gene is also active in Leishmania braziliensis and Leishmania major.
Laban
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Department of Tropical Public Health, Harvard School of Public Health, Boston, MA 02115.
Wirth
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D000818:Animals; D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics* / Q000378:metabolism; D002872:Chromosome Deletion; D015870:Gene Expression; D005796:Genes; D005798:Genes, Bacterial; D007894:Leishmania mexicana / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D011994:Recombinant Proteins / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection; D014404:Tubulin / Q000235:genetics
D011994:Recombinant Proteins; D014404:Tubulin; D015500:Chloramphenicol O-Acetyltransferase