Assignment of the coding sequence for carcinoembryonic antigen (CEA) and normal cross-reacting antigen (NCA) to human chromosome 19q13.
We have isolated and localized to chromosome 19 a genomic sequence for carcinoembryonic antigen (CEA). A human cosmid bank was screened with two degenerate oligonucleotide sequences corresponding to N-terminal segments of the protein. Sequence analysis of a selected cosmid has confirmed the presence of an exon representing the 107 amino acids of the first protein domain (plus part of a putative leader sequence). In situ hybridization of both the exon DNA sequence and a more extensive genomic fragment to replication banded chromosomes has indicated that CEA and strongly cross-hybridizing members of the CEA family can be assigned to 19q13. This conclusion is supported by studies with a somatic cell hybrid cell-line.
Willcocks
T C
TC
Department of Biochemistry, University of Oxford.
Craig
S P
SP
Craig
I W
IW
D000951:Antigens, Neoplasm; D001483:Base Sequence; D002272:Carcinoembryonic Antigen / Q000235:genetics*; D015815:Cell Adhesion Molecules; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19; D003360:Cosmids; D004247:DNA / Q000235:genetics; D005091:Exons; D005796:Genes; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping
D000951:Antigens, Neoplasm; D002272:Carcinoembryonic Antigen; D015815:Cell Adhesion Molecules; D006023:Glycoproteins; D004247:DNA