title: Comparison of Alternaria tenuis extracts prepared from different raw materials.
abstract: Alternaria extracts, prepared from three different sources under the same conditions, were compared by several biochemical/immunochemical methods. Two raw materials (A,G) contained mostly mycelia. The third raw material (C) contained mycelia (35%) and spores (65%). Extracts A and G were different in total allergenic potency, antigen/allergen pattern, and in the Alt 1 content. Extract C was of similar total allergenic potency as extract A but showed a somewhat different antigen/allergen pattern and a different Alt 1 content. Although there were some compositional differences among the extracts, all extracts regardless of source materials (spores and mycelia) demonstrated strong RAST inhibition activity and parallelism of the inhibition curves.
authors.affiliation: Allergopharma, Joachim Ganzer KG, Reinbek, FRG.
authors.forename: R
authors.initials: R
authors.lastname: Wahl
authors.forename: J D
authors.initials: JD
authors.lastname: Oliver
authors.forename: P R
authors.initials: PR
authors.lastname: Hauck
authors.forename: H G
authors.initials: HG
authors.lastname: Winter
authors.forename: H J
authors.initials: HJ
authors.lastname: Maasch
mesh_terms: D000485:Allergens / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification; D000528:Alternaria / Q000276:immunology*; D002241:Carbohydrates / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000032:analysis; D007123:Immunoelectrophoresis, Two-Dimensional; D007525:Isoelectric Focusing; D003904:Mitosporic Fungi / Q000276:immunology*; D011852:Radioallergosorbent Test
chemical_list: D000485:Allergens; D002241:Carbohydrates; D005656:Fungal Proteins