title: A new molecular form of PYY: structural characterization of human PYY(3-36) and PYY(1-36).
abstract: A radioimmunoassay was developed using an antibody raised in rabbits against synthetic porcine PYY. This radioimmunoassay was used to detect PYY immunoreactivity in human intestinal extracts. Human colonic mucosa was extracted with acid, centrifuged and the supernatant concentrated by low pressure preparative reverse phase chromatography. A subsequent C-18 reverse phase HPLC step separated two peaks of PYY immunoreactivity. Each peak was purified by sequential steps of ion-exchange FPLC and reverse phase HPLC. In the final purification step single absorbance peaks were associated with PYY immunoreactivity. Microsequence, amino acid, and mass spectral analysis of the intact and tryptic fragments of the two peptides were consistent with the structures: YPIKPEAPGEDASPEELNRYYASLRHYLNLVTRQRY-amide [human PYY(1-36)] and--IKPEAPGEDASPEELNRYYASLRHYLNLVTRQRY-amide [human PYY(3-36)]. Human PYY(1-36) differs from porcine PYY only at position 3, with Ile instead of Ala, and position 18, with Asn instead of Ser. PYY(3-36) may differ in its biological activity from the intact peptide. Its high proportions in the colon suggest that it is released into the circulation where it could act as a partial antagonist of PYY(1-36).
authors.affiliation: California Biotechnology Inc., Mountain View 94043.
authors.forename: G A
authors.initials: GA
authors.lastname: Eberlein
authors.forename: V E
authors.initials: VE
authors.lastname: Eysselein
authors.forename: M
authors.initials: M
authors.lastname: Schaeffer
authors.forename: P
authors.initials: P
authors.lastname: Layer
authors.forename: D
authors.initials: D
authors.lastname: Grandt
authors.forename: H
authors.initials: H
authors.lastname: Goebell
authors.forename: W
authors.initials: W
authors.lastname: Niebel
authors.forename: M
authors.initials: M
authors.lastname: Davis
authors.forename: T D
authors.initials: TD
authors.lastname: Lee
authors.forename: J E
authors.initials: JE
authors.lastname: Shively
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007422:Intestines / Q000032:analysis; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010446:Peptide Fragments; D019894:Peptide YY; D010455:Peptides / Q000032:analysis* / Q000302:isolation & purification; D011863:Radioimmunoassay; D013329:Structure-Activity Relationship; D014357:Trypsin
chemical_list: D000596:Amino Acids; D010446:Peptide Fragments; D010455:Peptides; D019894:Peptide YY; C061785:peptide YY (3-36); D014357:Trypsin