Effects of interferon and gangliosides on growth of cultured human glioma and fetal brain cells.
Human beta-interferon (IFN) induced an antiviral state in two fetal brain and six glioma cell lines. The growth-inhibitory effect of IFN was most pronounced on three glioblastoma lines and least on fetal brain and oligodendroglioma cells; IFN growth inhibition of one schwannoma and one anaplastic cell line was intermediate between the two other groups. Thus, the growth-inhibitory effect of IFN generally correlated with the degree of anaplasia of the tissue from which the cells were derived. IFN (1000 units/ml) had to be present for 24 to 48 hr to have a significant inhibitory effect on growth of glioblastoma (12-18) cells. However, growth inhibition of 12-18 cells exposed to IFN for 3 days persisted for 3 weeks. Both sialic acid-N-acetylgalactosamine ganglioside and a mixture of normal human brain gangliosides (50 microM) inhibited growth of fetal brain (CHII) but not glioblastoma 12-18 cells. However, preincubation of cells with either sialic acid-N-acetylgalactosamine or a mixture of gangliosides did not augment the growth-inhibitory effects of IFN on either CHII or 12-18. These results indicate that gangliosides and IFN may be operating through different mechanisms to cause growth inhibition.
Yates
A J
AJ
Stephens
R E
RE
Elder
P J
PJ
Markowitz
D L
DL
Rice
J M
JM
D001921:Brain / Q000166:cytology* / Q000187:drug effects; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D005333:Fetus; D005732:Gangliosides / Q000494:pharmacology*; D005910:Glioma / Q000188:drug therapy / Q000473:pathology*; D006801:Humans; D007372:Interferons / Q000494:pharmacology*; D011247:Pregnancy
D005732:Gangliosides; D007372:Interferons