Demonstration of inter- and intraspecies differences in serotonin binding sites by antibodies from an autistic child.
Serotonin (5-HT) binding sites from bovine and rat cerebral cortex membranes share pharmacological properties that allow both to be subclassified by the same criteria. We show here that [3H]5-HT binding sites from human cortex also possess pharmacological properties that follow the same subclassification scheme as for bovine and rat cortex. In addition, we show that solubilized 5-HT1 and 5-HT3 sites from all three species have an s20,w value of 3.4. Despite these similar pharmacological and physical characteristics, we can demonstrate antigenic differences between receptor types and species. Human 5-HT1A sites can be distinguished from human 5-HT1B, 5-HT2, and 5-HT3 sites and from equivalent sites in rat and bovine cortex. The anti-human 5-HT1A antibodies were discovered in the blood of an autistic child and may have clinical or etiologic significance for this disorder.
Todd
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Ciaranello
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RD
D000818:Animals; D001321:Autistic Disorder / Q000378:metabolism*; D001323:Autoantibodies / Q000032:analysis; D001665:Binding Sites; D002417:Cattle; D002540:Cerebral Cortex / Q000378:metabolism*; D002648:Child; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis; D005260:Female; D006801:Humans; D051381:Rats; D012701:Serotonin / Q000276:immunology / Q000378:metabolism*; D013045:Species Specificity
D001323:Autoantibodies; D000939:Epitopes; D012701:Serotonin