Hemagglutinin-neuraminidase glycoprotein as a determinant of pathogenicity in mumps virus hamster encephalitis: analysis of mutants selected with monoclonal antibodies.
With the aid of monoclonal antibodies directed against a specific site on the hemagglutinin-neuraminidase surface glycoprotein, four mutants of the Kilham neurotropic strain of mumps virus were isolated. All four mutants had increased neuraminidase activity. Two mutants (M10 and M12) lost their hemagglutination capacity with human O erythrocytes but retained their ability to agglutinate guinea pig erythrocytes at 4 degrees C. A third mutant (M11) showed a change in the molecular weight of the hemagglutinin-neuraminidase glycoprotein. These three mutants (M10, M11, and M12) showed unaltered capacity to infect tissue cultures and to cause encephalitis in newborn hamsters. A fourth mutant (M13) retained its hemagglutination activity and capacity to infect Vero cell cultures but showed significantly lower neurovirulence in the suckling hamster brain than did the parental Kilham strain and the other three mutants. Both the number of infected neurons and the amount of infectious virus in the brain was reduced. On the other hand, there were no apparent differences in the occurrence of viral antigen in ependymal cells, indicating a selective change in affinity for neurons in the brain. These results suggest that certain changes in the hemagglutinin-neuraminidase glycoprotein may lead to an alteration of the neuropathogenicity of the Kilham strain of mumps virus.
Löve
A
A
Rydbeck
R
R
Kristensson
K
K
Orvell
C
C
Norrby
E
E
D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D002460:Cell Line; D002522:Chlorocebus aethiops; D006224:Cricetinae; D004660:Encephalitis / Q000382:microbiology*; D000939:Epitopes / Q000032:analysis; D015707:HN Protein; D006382:Hemadsorption; D006386:Hemagglutination Tests; D007668:Kidney; D008970:Molecular Weight; D009109:Mumps virus / Q000472:pathogenicity*; D009154:Mutation; D009439:Neuraminidase / Q000032:analysis; D013045:Species Specificity; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics*
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000939:Epitopes; D015707:HN Protein; D014764:Viral Proteins; D009439:Neuraminidase