title: Neoplastic transformation of human epidermal keratinocytes by AD12-SV40 and Kirsten sarcoma viruses.
abstract: Recent investigations have begun to dissect the number and nature of genetic alterations associated with cancer cells. In the present study, primary human epidermal keratinocytes acquired indefinite life-span in culture but did not undergo malignant conversion in response to infection with a hybrid of adenovirus 12 and simian virus 40. Addition of Kirsten murine sarcoma virus, which contains a K-ras oncogene, to these cells induced morphological alterations associated with the acquisition of neoplastic properties. These findings demonstrate the malignant transformation of human primary epithelial cells in culture and support a multiple-step process for neoplastic conversion.
authors.forename: J S
authors.initials: JS
authors.lastname: Rhim
authors.forename: G
authors.initials: G
authors.lastname: Jay
authors.forename: P
authors.initials: P
authors.lastname: Arnstein
authors.forename: F M
authors.initials: FM
authors.lastname: Price
authors.forename: K K
authors.initials: KK
authors.lastname: Sanford
authors.forename: S A
authors.initials: SA
authors.lastname: Aaronson
mesh_terms: D000260:Adenoviruses, Human / Q000378:metabolism*; D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D004847:Epithelial Cells; D006801:Humans; D007633:Keratins; D007708:Kirsten murine sarcoma virus / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D008819:Mice, Nude; D009053:Sarcoma Viruses, Murine / Q000378:metabolism*; D012513:Sarcoma, Experimental / Q000378:metabolism; D013539:Simian virus 40 / Q000378:metabolism*; D012867:Skin / Q000166:cytology*
chemical_list: D007633:Keratins