HLA-DR-, MB- and novel DC-related determinants restrict purified protein derivative of tuberculin (PPD)-stimulated human T cell proliferation.
Class II major histocompatibility complex determinants restricting recognition of tuberculin antigens (purified protein derivative; PPD) were studied by using monoclonal antibodies (mAb) to block lymphoproliferative responses. Anti-class II mAb were shown to exert inhibitory effects at the level of the antigen-presenting cells, without inducing suppressive lymphocytes or macrophages. Using panels of HLA-typed antigen-presenting cells and nonalloreactive proliferative T cell lines, derived by limiting dilution, restriction elements for PPD responses appeared to correlate with the donor's HLA-DRw6 specificity (one clone), MB1 (one clone), MB3 (one clone), or no established class II (or class I) specificity (three clones). mAb TU22, reacting with nonpolymorphic DC-like determinants, strongly inhibited stimulation of all clones except that restricted by DR antigens, suggesting the DC-like character not only of the MB1- and MB3-associated, but also of the unassigned, restriction elements of these cloned lines. In contrast, stimulation of the DR-restricted line was strongly inhibited by DR/SB-specific mAb which only weakly inhibited the stimulation of clones restricted by DC-like determinants. These results suggest that clonally distributed PPD-reactive proliferative lymphocytes from a single donor may be restricted by at least three different class II determinants (HLA-DR, MB, or a second, novel, DC-related molecule).
Pawelec
G
G
Schneider
E M
EM
Müller
C
C
Wernet
P
P
D000911:Antibodies, Monoclonal / Q000502:physiology; D000918:Antibody Specificity; D000938:Antigen-Presenting Cells / Q000276:immunology; D001667:Binding, Competitive; D002999:Clone Cells / Q000276:immunology; D000939:Epitopes / Q000276:immunology*; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation; D013601:T-Lymphocytes / Q000276:immunology*; D014373:Tuberculin / Q000494:pharmacology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D014373:Tuberculin