Role of the viral and cellular encoded thymidine kinase in the repair of UV-irradiated herpes simplex virus.
A strain of herpes simplex type 1 (HSV-1:KOS) encoding a functional thymidine kinase (tk+) gene and a thymidine kinase deficient (tk-) mutant strain (HSV-1:PTK3B) were used as probes to examine the repair of UV-damaged viral DNA in one tk- (143) and two tk+ (R970-5 and AC4) human cell lines. UV survival for each HSV-1 strain was similar for infection of both tk- and tk+ cells suggesting that the repair of viral DNA was not dependent on the expression of a functional cellular tk gene. In contrast, UV survival of HSV-1:PTK3B was substantially reduced compared to HSV-1:KOS when infecting all 3 human cell lines, as well as Vero monkey kidney cells and LPM1A mouse cells. These results suggest that the repair of UV-irradiated HSV-1 in lytically infected mammalian cells depends, in part at least, on the expression of the viral encoded tk.
Rainbow
A J
AJ
Department of Biology, McMaster University, Hamilton, Ont., Canada.
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D004260:DNA Repair; D004279:DNA, Viral / Q000235:genetics / Q000528:radiation effects; D005814:Genes, Viral; D006561:Herpes Simplex / Q000235:genetics*; D006801:Humans; D051379:Mice; D013937:Thymidine Kinase / Q000502:physiology*; D014466:Ultraviolet Rays; D010948:Viral Plaque Assay; D015678:Viral Structural Proteins / Q000235:genetics
D004279:DNA, Viral; D015678:Viral Structural Proteins; D013937:Thymidine Kinase