Biphasic increase in the in vivo phosphorylation of nuclear 110-kDa protein during early lymphocyte transformation.
A major 110-kDa phosphoprotein in rat liver and hepatoma (P 110) was identified in nuclear 0.2 M HCl extracts from rat lymph node cells. Stimulation with concanavalin A altered both the amount of P110 and, more strikingly, its in vivo phosphorylation in a typical biphasic manner with an initial maximum after 10-14 h. RNA synthesis showed a similar biphasic increase. Inhibition of hnRNA synthesis (5,6 dichlorobenzimidazoleriboside), but not of DNA synthesis (hydroxyurea), depressed both the amount of P110 and its phosphorylation markedly.
Kostka
G
G
Schweiger
A
A
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism*; D002478:Cells, Cultured; D055598:Chemical Phenomena; D002621:Chemistry; D003208:Concanavalin A / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D006868:Hydrolysis; D008099:Liver / Q000378:metabolism*; D008113:Liver Neoplasms; D008198:Lymph Nodes; D008213:Lymphocyte Activation; D008968:Molecular Conformation; D010447:Peptide Hydrolases; D010750:Phosphoproteins / Q000302:isolation & purification*; D010766:Phosphorylation; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D013997:Time Factors
D010750:Phosphoproteins; D003208:Concanavalin A; D012313:RNA; D004247:DNA; D010447:Peptide Hydrolases