A digital image processing system for quantitating dynamic morphology in cultured mammalian cells.
An automated, video-driven system has been developed which can quantitate dynamic cell morphology in cultured mammalian cells. This system is based upon the Personal Image Analysis System and is assisted by a video-enhanced contrast microscopy with a computer-aided digital image processing unit and a time-lapse video technique. Various parameters for cell motility including locomotion (vectorial translation) and accompanying shape changes can be simultaneously analyzed. Here, we describe this system and demonstrate its application in Balb/c 3T3 cell culture. This system represents a new tool for exploring subtleties of mammalian cell behavior.
Tatsuka
M
M
Department of Oncogene Research, Osaka University, Japan.
Jinno
S
S
Owada
M K
MK
Kakunaga
T
T
D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure*; D002465:Cell Movement; D002478:Cells, Cultured; D003196:Computer Graphics; D046508:Culture Techniques / Q000295:instrumentation / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C; D014741:Video Recording / Q000295:instrumentation / Q000379:methods