Effect of the administration of cycloheximide to thioacetamide-primed rats or partial-hepatectomized rats on the RNA polymerase I activity in isolated nuclei of liver.
1. Twenty-four hours after administration of thioacetamide to normal rats, the activity and amount of RNA polymerase I in isolated liver nuclei were almost doubled. 2. When cycloheximide was administered to the drug-treated rats and normal rats 1 or 2 hr before death, the reduction in the activity was of the same degree, that is, about half of the activity of liver nuclei from normal rats. 3. Partial hepatectomy also caused about 2-fold increase in the RNA polymerase I activity in isolated liver nuclei after 16 hr, but the increased activity was almost completely abolished by injection of cycloheximide 1-3 hr before killing.
Suzuki
N
N
Effect of Pharmaceutical Sciences, Chiba University, Japan.
Matsui
H
H
Onodera
M
M
Hosoya
T
T
D000081:Acetamides / Q000494:pharmacology*; D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000201:enzymology; D003513:Cycloheximide / Q000494:pharmacology*; D006498:Hepatectomy; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D012318:RNA Polymerase I / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013853:Thioacetamide / Q000494:pharmacology*
D000081:Acetamides; D013853:Thioacetamide; D003513:Cycloheximide; D012318:RNA Polymerase I