Phylogenetic survey of proteins related to synapsin I and biochemical analysis of four such proteins from fish brain.
A phylogenetic survey of proteins immunologically related to Synapsin I, a major synaptic vesicle-associated phosphoprotein in mammals was carried out. Proteins antigenically related to Synapsin I were found by use of radioimmunoassay and other radioimmunochemical techniques in the nervous systems of several vertebrate and invertebrate species, which included birds, reptiles, amphibians, fish, echinoderms, arthropods, and mollusks. Four proteins present in fish brain, antigenically related to Synapsin I, were further studied and found to resemble mammalian Synapsin I in several respects. Like Synapsin I, the fish proteins were present in high amounts in nervous tissue, were enriched in synaptosomal fractions of brain where they were substrates for endogenous protein kinases, were acid extractable, and were sensitive to digestion by collagenase. In addition, two-dimensional peptide-mapping analysis revealed some homology between major phosphopeptide fragments of Synapsin I and the fish proteins. The results indicate that proteins related to Synapsin I are wide-spread in the animal kingdom.
Goelz
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Nestler
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EJ
Greengard
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P
D000818:Animals; D001001:Anura; D001048:Aplysia; D001181:Arthropods; D001923:Brain Chemistry; D002417:Cattle; D002645:Chickens; D000242:Cyclic AMP / Q000494:pharmacology; D003530:Cyprinidae / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology; D006054:Goldfish / Q000378:metabolism*; D007158:Immunologic Techniques; D007163:Immunosorbent Techniques; D003012:Microbial Collagenase / Q000378:metabolism; D009417:Nerve Tissue / Q000032:analysis; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D011817:Rabbits; D011863:Radioimmunoassay; D051381:Rats; D013215:Starfish; D016704:Synapsins; D014426:Turtles
D000939:Epitopes; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; D016704:Synapsins; D000242:Cyclic AMP; D011494:Protein Kinases; D003012:Microbial Collagenase