title: [Thyroglobulin gene expression in human thyroid cells in various types of thyroid pathology].
abstract: Highly purified thyroglobulin mRNA was isolated from human nodal euthyroid goiter. Full length cDNA was synthesized from 33S RNA by using reverse transcriptase in the presence of human placenta ribonuclease inhibitor. DNA complementary to human Tg mRNA was used in liquid hybridization experiments to determine the quantity of Tg mRNA. The amount of Tg mRNA in euthyroid nodal and congenital goiter was reduced. Tg specific mRNA was absent in thyroid cancer cells.
authors.forename: D A
authors.initials: DA
authors.lastname: Kadyrova
authors.forename: B A
authors.initials: BA
authors.lastname: Atakhanova
authors.forename: U P
authors.initials: UP
authors.lastname: Maksumova
authors.forename: Ia Kh
authors.initials: IaKh
authors.lastname: Turakulov
authors.forename: G I
authors.initials: GI
authors.lastname: Shipitsyna
mesh_terms: D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D015870:Gene Expression; D006044:Goiter, Nodular / Q000235:genetics; D006801:Humans; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D013954:Thyroglobulin / Q000235:genetics*; D013959:Thyroid Diseases / Q000235:genetics*; D013961:Thyroid Gland / Q000166:cytology; D014158:Transcription, Genetic; D014461:Ultracentrifugation
chemical_list: D012333:RNA, Messenger; D004247:DNA; D013954:Thyroglobulin
vernacular_title: Ekspressiia gena tireoglobulina v kletkakh shchitovidnoĭ zhelezy cheloveka pri nekotorykh vidakh tireoidnoĭ patologii.