Collagenolytic activity of the extracellular vesicles of Bacteroides gingivalis W50 and an avirulent variant W50/BE1.
The activities of the extracellular vesicle fractions of these two organisms were compared. Lytic activity against a native type I placental collagen substrate at 30 degrees C was assessed following sodium dodecyl sulphate-polyacrylamide gel electrophoresis and densitometry. A rapid rate of collagen depolymerization was achieved by the extracellular vesicle fraction of W50, yielding approx. 90% substrate degradation compared to 5% for W50/BE1 extracellular vesicles over 6 h incubation. The polypeptide digestion patterns produced by incubation with extracellular vesicle fractions of both organisms were identical, and similar to those yielded by incubation of substrate with whole W50 cells.
Smalley
J W
JW
Department of Dental Sciences, University of Liverpool, England.
Shuttleworth
C A
CA
Birss
A J
AJ
D001439:Bacteroides / Q000166:cytology / Q000201:enzymology* / Q000472:pathogenicity; D003094:Collagen / Q000378:metabolism*; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D006801:Humans
D003094:Collagen; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases