Confirmation of D-aspartic acid in the novel dipeptide beta-aspartylglycine isolated from tissue extract of Aplysia kurodai.
A novel o-phthalaldehyde-reactive compound was found in the h.p.l.c. chromatogram of Aplysia kurodai extract. This compound was isolated by ion-exchange chromatography and preparative high-voltage paper electrophoresis. It was shown by optical-rotatory-dispersion spectrum and optical-resolution h.p.l.c. analysis that this compound consisted of equimolar amounts of D-aspartic acid and glycine. This compound resisted cleavage in the Edman reaction. This peptide was inferred to be beta-D-aspartylglycine, and this was confirmed by synthesis. beta-D-Aspartylglycine was detected in all tissues of Aplysia kurodai, with especially high concentrations in body wall (skin and muscle) and gill.
Sato
M
M
School of Fisheries Sciences, Kitasato University, Iwate, Japan.
Yamaguchi
T
T
Kanno
N
N
Sato
Y
Y
D000818:Animals; D001048:Aplysia / Q000032:analysis*; D001224:Aspartic Acid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004151:Dipeptides / Q000032:analysis* / Q000138:chemical synthesis; D004590:Electrophoresis, Paper; D005998:Glycine / Q000032:analysis; D011487:Protein Conformation; D014018:Tissue Distribution
D004151:Dipeptides; D001224:Aspartic Acid; C018459:beta-aspartylglycine; D005998:Glycine