DNA homology of surface protein antigen A gene in mutans streptococci.
1. A recombinant plasmid, pYA724, containing an 8.45 kb DNA fragment encoding surface protein antigen A (spaA) from Streptococcus sobrinus 6715 was used to examine the DNA homology of the spaA gene with chromosomal DNA of various mutans streptococci strains. 2. Restriction endonuclease BamHI-digested pYA724 DNA was radio-labeled by nick-translation, and a DNA-DNA hybridization experiment was carried out. pYA724 DNA hybridized with chromosomal DNA of serotypes a, c, d, e, f and g strains, but not with b by dot DNA-hybridization and Southern blot DNA hybridization. 3. Chromosomal DNAs were isolated from several serotype c Streptococcus mutans strains, digested with BamHI, and analyzed by Southern blot DNA hybridization. pYA724 DNA hybridized with different sizes and numbers of BamHI-digested DNA fragments of the chromosomal DNAs. 4. These data indicated that all mutans streptococci strains except serotype b have DNA homologous with the spaA gene, although within the same serotype strain the spaA gene has a diversity of arrangement within the chromosome.
Abiko
Y
Y
Department of Biochemistry, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
Aoki
H
H
Sato
J
J
Koitabashi
M
M
Takiguchi
H
H
D000942:Antigens, Bacterial / Q000235:genetics*; D000954:Antigens, Surface / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D015139:Blotting, Southern; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D005798:Genes, Bacterial; D009693:Nucleic Acid Hybridization; D012689:Sequence Homology, Nucleic Acid; D013295:Streptococcus mutans / Q000235:genetics* / Q000276:immunology
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001426:Bacterial Proteins; D004269:DNA, Bacterial