title: Serodiagnosis of acute toxoplasmosis in macropods.
abstract: The sera of 34 Australian macropods, the brains of which had been bioassayed for Toxoplasma gondii, were used to establish that a titre greater than 1/32 was significant for a direct agglutination test against toxoplasmosis. In addition, the concentration of 2-mercaptoethanol required to destroy the IgM fraction of macropod serum was confirmed in a modified direct agglutination test. To further validate the tests, the serological responses of three eastern grey kangaroos (Macropus giganteus) dosed orally with T. gondii oocysts and one M. giganteus injected with T. gondii cysts were studied. The tests were then used to investigate a diagnosis of acute toxoplasmosis in four Tasmanian pademelons (Thylogale billardierii) clinically suspected of acquiring toxoplasmosis naturally. One hundred and fifty-one Bennett's wallabies (Macropus rufogriseus rufogriseus) and 85 T. billardierii were also tested to determine the prevalence of acute toxoplasmosis of macropods in the wild. Four percent of M. r. rufogriseus and 1.2% of T. billardierii possessed T. gondii-specific IgM in their sera.
authors.affiliation: Department of Clinical Microbiology, Flinders University of South Australia School of Medicine, Flinders Medical Centre, Bedford Park.
authors.forename: A M
authors.initials: AM
authors.lastname: Johnson
authors.forename: H
authors.initials: H
authors.lastname: Roberts
authors.forename: P
authors.initials: P
authors.lastname: Statham
authors.forename: B L
authors.initials: BL
authors.lastname: Munday
mesh_terms: D000208:Acute Disease; D000372:Agglutination Tests; D000818:Animals; D000835:Animals, Wild; D000913:Antibodies, Protozoan / Q000032:analysis; D001921:Brain / Q000469:parasitology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007075:Immunoglobulin M / Q000032:analysis; D007614:Macropodidae / Q000469:parasitology*; D008297:Male; D008394:Marsupialia / Q000469:parasitology*; D015995:Prevalence; D014122:Toxoplasma / Q000276:immunology / Q000302:isolation & purification; D014124:Toxoplasmosis, Animal / Q000175:diagnosis* / Q000453:epidemiology
chemical_list: D000913:Antibodies, Protozoan; D007075:Immunoglobulin M