title: Action of mycosubtilin on erythrocytes and artificial membranes.
abstract: Mycosubtilin has a strong lytic action upon erythrocytes. The haemolytic effect of the antibiotic is inhibited by free cholesterol as well as cholesterol included in liposomes of phosphatidylcholine-cholesterol. The antibiotic binding on artificial membranes was studied with radioactive bilayer vesicles of dipalmitoylphosphatidylcholine (DPPC), and DPPC-cholesterol:mycosubtilin destroyed both kinds of vesicles. The inhibition of the haemolytic effect of mycosubtilin by liposomes is a result of an interaction which diminishes the concentration of free cholesterol.
authors.affiliation: Laboratoire de Biochimie Microbienne (CNRS UA 1176), Université Claude Bernard, Villeurbanne, France.
authors.forename: F
authors.initials: F
authors.lastname: Besson
authors.forename: M J
authors.initials: MJ
authors.lastname: Quentin
authors.forename: G
authors.initials: G
authors.lastname: Michel
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine; D000935:Antifungal Agents / Q000494:pharmacology; D002784:Cholesterol / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D008074:Lipoproteins / Q000494:pharmacology; D008081:Liposomes; D008567:Membranes, Artificial
chemical_list: D000935:Antifungal Agents; D008074:Lipoproteins; D008081:Liposomes; D008567:Membranes, Artificial; C012448:mycosubtiline; D015060:1,2-Dipalmitoylphosphatidylcholine; D002784:Cholesterol