Comparison of two methods for the determination of the isoenzymes of alpha-amylase in serum and urine.
In this paper we describe our results of the determination of the isoenzymes of alpha-amylase in serum and urine with two monoclonal antibodies, using 4,6-ethylidene-protected 4-nitrophenylmaltoheptaoside as substrate. For comparison we have used the wheat-germ inhibitor method with blue starch as substrate. The differences observed between each method were small. The technique using monoclonal antibodies is easy and reliable and can, therefore, replace the wheat-germ inhibitor method.
Hafkenscheid
J C
JC
Laboratory for Clinical Chemistry, St. Radboud Hospital, University of Nijmegen, The Netherlands.
Hessels
M
M
Wetzels
F M
FM
D000911:Antibodies, Monoclonal / Q000032:analysis; D006801:Humans; D007527:Isoenzymes / Q000097:blood* / Q000652:urine; D008722:Methods; D014018:Tissue Distribution; D014909:Wheat Germ Agglutinins; D000516:alpha-Amylases / Q000097:blood* / Q000652:urine
D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D014909:Wheat Germ Agglutinins; D000516:alpha-Amylases