The V-J-intergenic region of the human kappa locus.
The 23-kb region between the V kappa and J kappa gene clusters was investigated in some detail. The region was found to be free of V kappa genes or V kappa gene-like structures, confirming the previous supposition that the V kappa gene B3 is the J kappa proximal V kappa gene. The B3-J kappa distance of 23 kb was found to be the same in the DNAs of several individuals. A HindIII restriction fragment length polymorphism was detected within this region. A sequence of 533 bp located approximately in the middle of the region has a highly homologous counterpart (called homox) on another chromosome. The two sequences are 96% identical. Possible mechanisms for the generation of such a duplicate are discussed.
Klobeck
H G
HG
Institut für Physiologische Chemie, Physikalische Biochemie und Zellbiologie der Universität München.
Combriato
G
G
Zachau
H G
HG
D001483:Base Sequence; D003001:Cloning, Molecular; D015247:Deoxyribonuclease HindIII; D005803:Genes, Immunoglobulin / Q000235:genetics*; D006801:Humans; D007127:Immunoglobulin Constant Regions / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D007127:Immunoglobulin Constant Regions; D007135:Immunoglobulin Variable Region; D015247:Deoxyribonuclease HindIII