Reduced number of anionic sites is associated with glomerular basement membrane thickening in the diabetic BB-rat.
Glomerular basement membranes of diabetic and age- and sex-matched non-diabetic BB rats were studied morphometrically and ultrastructurally using a quantitative histochemical technique employing the cationic dye cuprolinic blue. Six months of diabetes resulted in a significant reduction in the density of anionic sites associated with increased thickness of the glomerular basement membrane. These findings suggest that the loss of anionic sites may be an important mechanism in the genesis of glomerular basement membrane dysfunction in diabetes.
Chakrabarti
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S
Department of Pathology, University of Manitoba, Winnipeg, Canada.
Ma
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Sima
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D000818:Animals; D000838:Anions; D001485:Basement Membrane / Q000473:pathology / Q000648:ultrastructure; D001786:Blood Glucose / Q000032:analysis; D004396:Coloring Agents; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000473:pathology*; D006442:Glycated Hemoglobin / Q000032:analysis; D007211:Indoles; D007678:Kidney Glomerulus / Q000473:pathology* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D009942:Organometallic Compounds; D051381:Rats; D011913:Rats, Inbred BB; D012016:Reference Values
D000838:Anions; D001786:Blood Glucose; D004396:Coloring Agents; D006442:Glycated Hemoglobin; D007211:Indoles; D009942:Organometallic Compounds; C015445:copper phthalocyanine