Autoregulation of RNase III operon by mRNA processing.
RNase III has been implicated in the control of gene expression by the processing of mRNA. We have found that the rnc operon is autoregulated; rnc- mutant strains oversynthesize the operon's mRNA and protein products. A site in the 5'-noncoding region of the operon's message is cleaved by RNase III. This site-specific cleavage appears to be the initial step in the functional inactivation of the message, since the half-life of the cut message is dramatically shorter than that of the uncut message.
Bardwell
J C
JC
Laboratory of Chromosome Biology, Frederick Cancer Research Facility, MD 21701.
Régnier
P
P
Chen
S M
SM
Nakamura
Y
Y
Grunberg-Manago
M
M
Court
D L
DL
D001483:Base Sequence; D004587:Electrophoresis, Agar Gel; D004722:Endoribonucleases / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D006207:Half-Life; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D009876:Operon; D011233:Precipitin Tests; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D015183:Restriction Mapping; D043244:Ribonuclease III; D014158:Transcription, Genetic
D012333:RNA, Messenger; D004722:Endoribonucleases; D043244:Ribonuclease III