[Effect of iodoform on development of cultured tooth-germs of newborn Syrian hamsters].
To examine the effect of iodoform, widely used as a component of filling sealer for root canals, on differentiation and development of tooth-germs, tooth-germs of second molars from newborn Syrian hamsters were cultivated overnight and treated with various concentrations of iodoform for 48 hours. They were subsequently transplanted into the cheek pouch of Syrian hamsters and were subjected to histo chemical examinations three weeks later. 86% of the seven tooth germs examined survived treatment with 1.0 microgram/ml iodoform, as determined by morphology of tooth-germs. The ratio of survival decreased with increasing doses of iodoform, e.g. 63%, 37%, and 13% for 3.3 micrograms/ml, 6.6 micrograms/ml and 10.0 micrograms/ml iodoform, respectively. In untreated tooth-germs, a similar level of survival was obtained with treatment using 1.0 microgram/ml iodoform. Although treatment with 1.0 microgram/ml iodoform showed a survival similar to untreated tooth-germs, the treatment induced a considerable level of aplasia of enamel and dentin in the dental crowns. Meanwhile, higher dose of iodoform was required for induction of aplasia or depression of the dental pulp or root dentain. These results indicate that iodoform over the dose of 1.0 microgram/ml is toxic to cultured tooth-germs of Syrian hamsters and inhibits differentiation or development of the tooth-germs with tissue specificity.
Suzuki
K
K
D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae; D006847:Hydrocarbons, Iodinated / Q000633:toxicity*; D008647:Mesocricetus; D014083:Tooth Germ / Q000187:drug effects*
D006847:Hydrocarbons, Iodinated; C010473:iodoform