Magnetic cell sorting using colloidal protein-magnetite.
Magnetic filtration of labelled cells as a way of separating leucocyte subpopulations was tested with a very simple and easy filtration device, using colloidal magnetite as the labelling reagent. In order to quantitate cell enrichment, a double label (both fluorescent and magnetic) was used, under conditions which labelled less than 10% of the cells in the initial sample. Up to 20 million cells were simply passed through a small magnetic filter with a hand-held syringe. Depletion of labelled cells in the suspension that passed through was threefold, and enrichment of labelled cells in the wash of the filter after its removal from the magnet was approximately fivefold. Factors which limited the quality of separation are discussed. Other, more preliminary, experiments found enrichments of 15- to 30-fold with the same colloidal magnetite and hand-held apparatus when the cell labelling system was more selective.
Owen
C S
CS
Department of Biochemistry and Molecular Biology, Jefferson Medical College, Philadelphia, PA 19107.
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D003102:Colloids; D052203:Ferrosoferric Oxide; D007501:Iron; D007962:Leukocytes / Q000166:cytology; D008280:Magnetics; D051379:Mice; D010087:Oxides; D011506:Proteins
D003102:Colloids; D010087:Oxides; D011506:Proteins; D007501:Iron; D052203:Ferrosoferric Oxide