[Transport of monovalent cations and calcium in erythrocytes of rats with spontaneous hypertension: study with fractions enriched with young and old cells].
The erythrocytes from two spontaneously hypertensive rat strains (SHR and MHS) differ from those of control strains such as WKY and MNS in showing lower values of the mean volume and higher Na,K-cotransport rates. After 4-hour incubation in the presence of orthovanadate 45Ca levels in the erythrocytes from SHR was twice as high as in those from WKY. Under the same conditions, hemoglobin crystallization occurred, this process in SHR erythrocytes took place more intensively. No difference was found in these parameters between MNS and MHS. The fractions enriched with young and old cells remained to display variations in the mean volume of cells, Na,K-cotransport rates, 45Ca levels and hemoglobin crystallization, which were recorded between SHR and WKY. The WKY-SHR hybrids of second generation indicated that only Na,K-cotransport rates of the parameters examined positively correlated with blood pressures. From the findings it was concluded that the differences in the volume of erythrocytes and their permeability for cations were not due to the changed time course of cell aging in the blood bed, but to the specific characteristics of cytoskeleton protein arrangement, which were primarily typical of a rat reticulocytic membrane.
Pokudin
N I
NI
Orlov
S N
SN
Kotelevtsev
Iu V
IuV
Postnov
Iu V
IuV
D000818:Animals; D001692:Biological Transport; D002118:Calcium / Q000097:blood*; D002352:Carrier Proteins / Q000097:blood*; D002458:Cell Fractionation; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000378:metabolism*; D006973:Hypertension / Q000097:blood*; D008297:Male; D051381:Rats; D011918:Rats, Inbred SHR; D011919:Rats, Inbred Strains; D028021:Sodium-Potassium-Chloride Symporters
D002352:Carrier Proteins; D028021:Sodium-Potassium-Chloride Symporters; D002118:Calcium
Transport odnovalentnykh kationov i ka'ltsiia v éritrotsitakh krys so spontannoĭ gipertenzieĭ: issledovanie na fraktsiiakh, obogashchennykh molodymi i starymi kletkami.