Acrosomal status quantitation in human sperm.
Acceptable methods are currently available for the rapid quantitation of acrosomal status in human sperm. Application of these methods to human sperm has led to the concept that spontaneous acrosomal loss occurs infrequently during capacitation. Induced loss occurs following sperm exposure to human follicular fluid, to zonae- or cumulus-enclosed eggs, or to calcium ionophores or other agents that modulate intracellular calcium levels. Acrosomal status quantitation may be helpful in sperm morphologic characterization or in evaluating cryodamage but has not yet been established in fertility potential measurement.
Wolf
D P
DP
Division of Reproductive Biology and Behavior, Oregon Regional Primate Research Center, Beaverton 97006.
D000177:Acrosome / Q000502:physiology / Q000648:ultrastructure*; D002470:Cell Survival; D005260:Female; D005615:Freezing; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D013075:Sperm Capacitation; D013081:Sperm Motility; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000648:ultrastructure*