title: Internal entry of ribosomes and ribosomal scanning involved in hepatitis B virus P gene expression.
abstract: The recent demonstration that the synthesis of duck hepatitis B virus (HBV) reverse transcriptase does not require translational frameshifting and the finding that poliovirus mRNA translation occurs in a cap-independent manner by internal binding of ribosomes in the 5' noncoding region led us to design experiments to test the hypothesis of internal entry of ribosomes on C gene mRNA for HBV P gene expression. We show that in human cells, translation can be initiated at the first AUG of the HBV P gene by entry of ribosomes in a region located upstream of the P gene. Moreover, the leaky scanning of ribosomes observed on the first AUG of the HBV P gene could be responsible for the synthesis of the two forms of reverse transcriptase described for HBV particles.
authors.affiliation: Laboratoire de Biologie Moléculaire de la Réplication, Centre National de la Recherche Scientifique ER-272, Villejuif, France.
authors.forename: O
authors.initials: O
authors.lastname: Jean-Jean
authors.forename: T
authors.initials: T
authors.lastname: Weimer
authors.forename: A M
authors.initials: AM
authors.lastname: de Recondo
authors.forename: H
authors.initials: H
authors.lastname: Will
authors.forename: J M
authors.initials: JM
authors.lastname: Rossignol
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D004274:DNA, Recombinant / Q000378:metabolism; D015870:Gene Expression; D005814:Genes, Viral; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D008979:Moloney murine leukemia virus / Q000235:genetics; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D012270:Ribosomes / Q000378:metabolism*; D014158:Transcription, Genetic; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics; D015678:Viral Structural Proteins / Q000235:genetics*
chemical_list: D004274:DNA, Recombinant; D012333:RNA, Messenger; D014764:Viral Proteins; D015678:Viral Structural Proteins