Elongation arrest is not a prerequisite for secretory protein translocation across the microsomal membrane.
Signal recognition particle (SRP) is a ribonucleoprotein consisting of six distinct polypeptides and one molecule of small cytoplasmic 7SL RNA. It was previously shown to promote the co-translational translocation of secretory proteins across the endoplasmic reticulum by (a) arresting the elongation of the presecretory nascent chain at a specific point, and (b) interacting with the SRP receptor, an integral membrane protein of the endoplasmic reticulum which is active in releasing the elongation arrest. Recently a procedure was designed by which the particle could be disassembled into its protein and RNA components. We have further separated the SRP proteins into four homogeneous fractions. When recombined with each other and with 7SL RNA, they formed fully active SRP. Particles missing specific proteins were assembled in the hope that some of these would retain some functional activity. SRP(-9/14), the particle lacking the 9-kD and 14-kD polypeptides, was fully active in promoting translocation, but was completely inactive in elongation arrest. This implied that elongation arrest is not a prerequisite for protein translocation. SRP receptor was required for SRP(-9/14)-mediated translocation to occur, and thus must play some role in the translocation process in addition to releasing the elongation arrest.
Siegel
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Walter
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D000818:Animals; D001692:Biological Transport; D002417:Cattle; D007425:Intracellular Membranes / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism*; D010441:Peptide Chain Elongation, Translational; D010446:Peptide Fragments / Q000302:isolation & purification / Q000502:physiology; D010455:Peptides / Q000302:isolation & purification / Q000502:physiology*; D014176:Protein Biosynthesis; D021382:Protein Sorting Signals; D011506:Proteins / Q000378:metabolism; D012313:RNA / Q000302:isolation & purification; D011817:Rabbits; D011956:Receptors, Cell Surface / Q000502:physiology; D018160:Receptors, Cytoplasmic and Nuclear; D018000:Receptors, Peptide; D013997:Time Factors
D010446:Peptide Fragments; D010455:Peptides; D021382:Protein Sorting Signals; D011506:Proteins; D011956:Receptors, Cell Surface; D018160:Receptors, Cytoplasmic and Nuclear; D018000:Receptors, Peptide; C036318:signal peptide receptor; D012313:RNA