title: Effects of TGF-beta on retinal pigmented epithelium in vitro.
abstract: Embryonic chick retinal pigmented epithelium (RPE) has been grown on glass derivatized with covalently bound proteins of basement membrane and treated with transforming growth factor-beta (TGF-beta). In the present paper we show that over the concentration range tested (0.1-10 ng/ml) TGF-beta has no effect on RPE cell proliferation either in the presence or the absence of serum, cell motility and the organization of cytoskeleton-extracellular matrix linkage complexes with respect to their structure and presence of actin, vinculin, talin, integrin and fibronectin. The protein profiles of total cell/ECM extracts of cells grown in the presence or the absence of TGF-beta are similar although some stimulation of protein synthesis and of production of fibronectin-containing extracellular matrix has been detected.
authors.affiliation: Department of Anatomy, University of Toronto, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Opas
authors.forename: E
authors.initials: E
authors.lastname: Dziak
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D003599:Cytoskeleton / Q000187:drug effects; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000187:drug effects*; D015291:Transforming Growth Factors / Q000494:pharmacology*
chemical_list: D015291:Transforming Growth Factors