title: The correlation between cell survival curve and dose response curve of micronucleus (MN) frequency.
abstract: The correlation between cell survival curve and dose response curve of MN frequency following irradiations was studied using cytokinesis-block method. Both dose response curves were analyzed by linear quadratic model, i.e. SF = exp (-alpha D-beta D2) and MN frequency = aD + bD2 + C. A good correlation between alpha/beta and a/b ratios was observed in repeated paired experiments (= 0.97). When the cells were treated with BUdR, alpha-type radiosensitizer, a value in dose response curve of MN frequency increased but b value did not. These data present that MN frequency assay using cytokinesis-block method is available as a tool of rapid assay of radiosensitivity of cells.
authors.affiliation: Department of Radiology, Faculty of Medicine, Kyoto University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ono
authors.forename: E
authors.initials: E
authors.lastname: Wandl
authors.forename: K
authors.initials: K
authors.lastname: Tsutsui
authors.forename: K
authors.initials: K
authors.lastname: Sasai
authors.forename: M
authors.initials: M
authors.lastname: Abe
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002455:Cell Division; D002470:Cell Survival / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D015162:Micronucleus Tests; D008954:Models, Biological; D011829:Radiation Dosage; D014407:Tumor Cells, Cultured / Q000528:radiation effects*
chemical_list: D001973:Bromodeoxyuridine