An analysis of myo-[3H]inositol trisphosphates found in myo-[3H]inositol prelabelled avian erythrocytes.
Evidence is presented to show that acid extracts of avian erythrocytes prelabelled for 24-48 h with myo-[3H]inositol contain the following myo-[3H]inositol trisphosphates (expressed as a percentage of total myo-[3H]inositol trisphosphates extracted): 36% myo-[3H]inositol 1,4,5-trisphosphate; 33.7% myo-[3H]inositol 1,3,4-trisphosphate; 13% myo-[3H]inositol 3,4,5-trisphosphate; 9.7% myo-[3H]inositol 3,4,6-trisphosphate; 4.4% myo-[3H]inositol 1,4,6-trisphosphate and 3.3% myo-[3H]inositol 1,3,6-trisphosphate. The only phosphatidyl-myo-[3H]inositol bisphosphate that could be detected in [3H]Ins-prelabelled avian erythrocytes was phosphatidyl-myo-[3H]inositol 4,5-bisphosphate. Cellular myo-[3H]inositol 3,4,5-trisphosphate may be synthesized by dephosphorylation of myo-[3H]inositol 3,4,5,6-tetrakisphosphate. D- and L-myo-[3H]inositol 1,4,6-trisphosphate and D- and L-myo-[3H]inositol 1,3,6-trisphosphate may be dephosphorylation products of myo-[3H]inositol 1,3,4,6-tetrakisphosphate.
Stephens
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LR
Smith Kline and French Research Limited, The Frythe, Welwyn, Herts., U.K.
Hawkins
P T
PT
Downes
C P
CP
D000818:Animals; D002645:Chickens; D004912:Erythrocytes / Q000378:metabolism; D007294:Inositol / Q000097:blood*; D015544:Inositol 1,4,5-Trisphosphate / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood; D007064:L-Iditol 2-Dehydrogenase / Q000378:metabolism; D008297:Male; D051381:Rats; D013237:Stereoisomerism; D013402:Sugar Alcohols / Q000378:metabolism; D014316:Tritium
D013402:Sugar Alcohols; D014316:Tritium; D007294:Inositol; D015544:Inositol 1,4,5-Trisphosphate; D007064:L-Iditol 2-Dehydrogenase