A method for the covalent attachment of cells to glass slides for use in immunohistochemical assays.
A method for covalently attaching viable cells to glass slides is described. The cells are linked covalently to the slide without the use of drying or other fixation procedures that normally destroy cellular surface antigens. The attachment is permanent, and the slides with attached cells may be cryopreserved. The use of the avidin-biotin-peroxidase procedure with this procedure shows excellent sensitivity comparable to flow cytometry.
Maples
J A
JA
D000911:Antibodies, Monoclonal; D002448:Cell Adhesion; D005434:Flow Cytometry; D005615:Freezing; D005898:Glass; D006651:Histocytochemistry / Q000379:methods*; D006801:Humans; D007120:Immunochemistry / Q000379:methods*; D007124:Immunoenzyme Techniques; D011309:Preservation, Biological; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal