Standardization of allergenic extracts of Aspergillus fumigatus. Liberation of IgE-binding components during cultivation.
During cultivation of Aspergillus fumigatus a rapid liberation of IgE-binding components was found reaching maximum values during the logarithmic phase of growth (phase I). After a fall in IgE-binding titers during phase II, appearance of additional IgE-binding components was noted during the period of lysis of the microorganism (phase III). These latter allergenic components are different from the phase I IgE-binding components, as was shown by crossed-inhibition studies. The number of precipitating antigenic components was not related with the corresponding IgE-binding titers and showed an increase during all phases of growth. The rapid changes in both IgE- and IgG-binding properties and the discrepancies between precipitating properties and IgE binding are discussed in relation to standardization and quality control of aspergillus extracts.
Kauffman
H F
HF
van der Heide
S
S
van der Laan
S
S
Hovenga
H
H
Beaumont
F
F
de Vries
K
K
D000485:Allergens / Q000592:standards*; D000946:Antigens, Fungal / Q000592:standards; D001232:Aspergillus fumigatus / Q000254:growth & development / Q000276:immunology*; D001666:Binding Sites, Antibody; D046508:Culture Techniques; D000939:Epitopes; D006863:Hydrogen-Ion Concentration; D007122:Immunoelectrophoresis; D007073:Immunoglobulin E / Q000276:immunology; D014020:Tissue Extracts / Q000592:standards*
D000485:Allergens; D000946:Antigens, Fungal; D000939:Epitopes; D014020:Tissue Extracts; D007073:Immunoglobulin E