Adaptation of the May-Grünwald-Giemsa staining method for automated differential counting of blood leukocytes by a Hematrak analyzer.
The selection of exact pH (6.85/6.90) concentrations of the stains and staining times made it possible to use the May-Grünwald-Giemsa (MGG) staining method for smears studied with a Hematrak model 240 automated differential leucocyte counter. The differences between the smears stained by the method of Wright according to the recommendations of the manufacturer and the present MGG-modification resulted in non-significant differences in the recognition of various types of leucocytes. The differences between the manual method and those obtained by Hematrak for the MGG-stained samples with normal total leucocyte counts were nonsignificant with all types of leucocytes except basophils, which were underestimated by the instrument. The present staining procedure was adequate in studying of neutrophilias. When samples with lymphocytosis or with immature cells were examined, too few lymphocytes and immature cells and too many monocytes were obtained by the instrument, as compared to the manual method.
Penttilä
I M
IM
Mahlamäki
E
E
Mononen
I
I
Kärkkäinen
P
P
D001331:Automation; D001399:Azure Stains; D006801:Humans; D007958:Leukocyte Count / Q000295:instrumentation*; D013194:Staining and Labeling / Q000379:methods*
D001399:Azure Stains