title: [Heterogeneous distribution of influenza A matrix protein in polyacrylamide gel, detected using an immunoblotting method with monoclonal antibodies].
abstract: The immune blotting method using monoclonal antibodies to Ml protein showed protein Ml to migrate in several bands in polyacrylamide gel electrophoresis (PGE) of influenza A virus polypeptides. The heterogeneous distribution of Ml protein in PGE is due to the formation of aggregates: dimers, trimers, and polymers of a higher order. The capacity of Ml protein for aggregation is typical not of all influenza virus strains and most likely is not associated with gel overloading. Since dimers and trimers of Ml protein comigrate in the gel with virus-specific proteins such as NP and proteins of a polymerase complex, this circumstance should be take into consideration in using PGE for isolation of pure influenza virus proteins.
authors.forename: M L
authors.initials: ML
authors.lastname: Khristova
authors.forename: T L
authors.initials: TL
authors.lastname: Busse
authors.forename: N V
authors.initials: NV
authors.lastname: Cheliapov
authors.forename: D
authors.initials: D
authors.lastname: Bucker
authors.forename: I G
authors.initials: IG
authors.lastname: Kharitonenkov
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D002642:Chick Embryo; D004591:Electrophoresis, Polyacrylamide Gel; D009980:Influenza A virus / Q000378:metabolism*; D009698:Nucleoproteins / Q000302:isolation & purification; D014763:Viral Matrix Proteins / Q000302:isolation & purification*
chemical_list: D000911:Antibodies, Monoclonal; D009698:Nucleoproteins; D014763:Viral Matrix Proteins
vernacular_title: Geterogennoe raspredelenie v poliakrilamidnom gele matriksnogo belka virusa grippa A, vyiavliaemoe metodom immunoblota pri ispolzovanii monoklonal'nykh antitel.