Sodium butyrate enhances the activities of membranal enzymes and increases drug sensitivity in a cell line from ascitic fluid of an ovarian carcinoma patient.
The effect of sodium butyrate was examined on the growth and phenotypic expression of a cell line derived from the ascitic fluid of an untreated patient with ovarian carcinoma. The chemical inducer of differentiation, sodium butyrate, markedly enhances the activity of the membrane-bound glycoprotein enzymes, alkaline phosphatase and gamma-glutamyl transpeptidase. The alkaline phosphatase corresponds to placental Regan type. Sodium butyrate (1 mM) alone has only a small inhibitory effect on cell growth. However, it was shown to potentiate the anti-proliferative effect of Adriamycin and to render the cells sensitive to cis-platinum.
Wasserman
L
L
Rogoff Research Institute, Beilinson Medical Center, Petah-Tikva, Israel.
Beery
E
E
Aviram
R
R
Levavi
H
H
Ovadia
J
J
Novogrodsky
A
A
Nordenberg
J
J
D000469:Alkaline Phosphatase / Q000378:metabolism*; D001202:Ascitic Fluid / Q000473:pathology; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D002945:Cisplatin / Q000494:pharmacology; D004317:Doxorubicin / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D004357:Drug Synergism; D005260:Female; D006801:Humans; D010051:Ovarian Neoplasms / Q000188:drug therapy / Q000201:enzymology* / Q000648:ultrastructure; D005723:gamma-Glutamyltransferase / Q000378:metabolism*
D002087:Butyrates; D020148:Butyric Acid; D004317:Doxorubicin; D005723:gamma-Glutamyltransferase; D000469:Alkaline Phosphatase; D002945:Cisplatin