Effects of ergocalciferol supplementation on the concentration of vitamin D and its metabolites in human milk.
The effect of maternal ergocalciferol (vitamin D2) supplementation on the concentrations of vitamin D, 25-hydroxyvitamin D (25-OH-D), 24R,25-dihydroxyvitamin D [24,25-(OH)2D], and 1 alpha,25-dihydroxyvitamin D [1,25-(OH)2D] in their milk was studied. Vitamin D2, D3, 25-OH-D2 and 25-OH-D3 were simultaneously determined by high performance liquid chromatography, and the determination of 24,25-(OH)2D and 1,25-(OH)2D was performed by competitive protein binding assay and radioreceptor assay, respectively, after separation of the D2 and D3 compounds. After healthy lactating mothers had received a daily oral dose of vitamin D2 (1,200 IU/d) for 4 wk, the concentrations of vitamin D2, D3 and the metabolites were determined in their plasma and milk. Although the plasma levels of 25-OH-D2 were significantly increased, the increase in milk was relatively small. On the other hand, the increase of vitamin D2 levels in milk was greater than that of 25-OH-D2 in milk after supplementation. The levels of 1,25-(OH)2D in milk was lower after 5 wk of lactation than after 1 wk of lactation, regardless of maternal vitamin D2 supplementation. When total antirachitic activities in milk were calculated, only a very slight increase was observed as a result of supplementation.
Takeuchi
A
A
Department of Hygienic Sciences, Kobe Women's College of Pharmacy, Japan.
Okano
T
T
Tsugawa
N
N
Tasaka
Y
Y
Kobayashi
T
T
Kodama
S
S
Matsuo
T
T
D015650:24,25-Dihydroxyvitamin D 3 / Q000097:blood / Q000378:metabolism; D015652:25-Hydroxyvitamin D 2 / Q000097:blood / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004872:Ergocalciferols / Q000097:blood / Q000378:metabolism / Q000494:pharmacology*; D005260:Female; D005527:Food, Fortified; D006801:Humans; D008895:Milk, Human / Q000378:metabolism*; D010758:Phosphorus / Q000378:metabolism; D011247:Pregnancy; D014807:Vitamin D / Q000097:blood / Q000378:metabolism*
D004872:Ergocalciferols; D014807:Vitamin D; D015652:25-Hydroxyvitamin D 2; D010758:Phosphorus; D015650:24,25-Dihydroxyvitamin D 3; C003564:1,25-dihydroxyergocalciferol; D002118:Calcium