Comparative results with five cannabinoid immunoassay systems at the screening threshold of 100 micrograms/L.
Five commercially available immunoassay techniques were compared for the detection of cannabinoid metabolites in urine at a positive cutoff of 100 micrograms/L. Numerical values, available for four of these techniques, were used to evaluate inter-assay equivalence at this threshold. The total number of positive results differed for each assay, with some individual samples exhibiting wide variations in numerical values. As a group, the radioimmunoassay systems gave a greater proportion of positive specimens than did enzyme-linked or fluorescence-polarization-based techniques. The single threshold of detection suggested by federal guidelines does not provide for equivalent screening of cannabinoids with these immunoassay techniques.
Wells
D J
DJ
Department of Pathology, University of South Alabama Medical Center, Mobile 36617.
Barnhill
M T
MT
D002186:Cannabinoids / Q000652:urine*; D004797:Enzyme-Linked Immunosorbent Assay; D005454:Fluorescence Polarization; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007118:Immunoassay; D011863:Radioimmunoassay; D011933:Reagent Kits, Diagnostic
D002186:Cannabinoids; D011933:Reagent Kits, Diagnostic