An assay for GDP-D-mannose-4,6-dehydratase.
The mechanism of GDP-D-mannose-4,6-dehydratase action with respect to loss of the C5 hydrogen has been established using GDP-D-[5-3H]-mannose as a substrate. This observation has been incorporated into a rapid assay for the enzyme based on the equilibration of 3H with the aqueous medium.
Chang
S
S
Broschat
K O
KO
Serif
G S
GS
D006155:Guanosine Diphosphate Mannose; D006836:Hydro-Lyases / Q000378:metabolism*; D014316:Tritium; D014867:Water
D014867:Water; D014316:Tritium; D006155:Guanosine Diphosphate Mannose; D006836:Hydro-Lyases; C044971:GDPmannose 4,6-dehydratase