title: In situ hybridization analysis of the somatostatin-containing neuron system in developing cerebellum of rats.
abstract: We used in situ hybridization histochemistry to examine the postnatal development of the somatostatin (SRIF) synthesizing system in the cerebellum of rats. There are numerous hybridizing neurons from 1 to 9 days after birth. These occur throughout the cerebellum including the developing medulla and cortex except in the external granular cell layer. The lateral cerebellar nucleus also contains SRIF gene-containing cells. The intensity of the signals for SRIF mRNA in the cerebellum decreases with age. There is a drastic decrease in SRIF mRNA in the lateral cerebellar nucleus. SRIF cells cannot be detected in the lateral cerebellar nucleus of adult rats, whereas a small, yet significant number of SRIF cells are scattered in the cerebellar medulla. However, the cerebellum of adult rats still contains a significant number of labeled cells in the granular cell layer, although the intensity for SRIF mRNA decreases from 14 days after birth to adulthood. SRIF gene-expressing cells in the cerebellar cortex are located primarily in the granular cell layer and appear to correspond to Golgi cells judging from their characteristic features. These results are consistent with our previous immunohistochemical study on the decrease of SRIF immunoreactivity in the cerebellum of adult rats. These findings, together with a recent study of transient SRIF receptor-expressing cells in the developing cerebellum suggest that SRIF acts during cerebellar development.
authors.affiliation: First Department of Anatomy, Osaka City University Medical School, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Inagaki
authors.forename: S
authors.initials: S
authors.lastname: Shiosaka
authors.forename: M
authors.initials: M
authors.lastname: Sekitani
authors.forename: K
authors.initials: K
authors.lastname: Noguchi
authors.forename: S
authors.initials: S
authors.lastname: Shimada
authors.forename: H
authors.initials: H
authors.lastname: Takagi
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D002531:Cerebellum / Q000254:growth & development / Q000378:metabolism*; D005786:Gene Expression Regulation; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013004:Somatostatin / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D013004:Somatostatin