One-step purification by high-performance liquid chromatography of retinal S-antigen.
A number of subsequent chromatographic techniques are commonly used, over a period of a few days, in order to obtain pure retinal S-antigen (S-Ag), a 50,000-dalton molecular weight protein with strong uveitopathogenic properties. A rapid high-performance liquid chromatographic technique employing a TSK-G3000 SWG gel-filtration column is described for the purification of bovine S-Ag. This single chromatographic step produced S-Ag preparations of high purity and recovery when compared to the conventionally isolated S-Ag.
Molinotti
C
C
Istituto di Clinica Oculistica, Università degli Studi di Bologna.
Puddu
P
P
Perugini
S
S
Barboni
P
P
Biagi
R
R
D000818:Animals; D000941:Antigens / Q000276:immunology / Q000302:isolation & purification*; D019393:Arrestin; D001327:Autoimmune Diseases / Q000209:etiology; D002417:Cattle; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005136:Eye Proteins / Q000276:immunology / Q000302:isolation & purification*; D005260:Female; D051381:Rats; D011919:Rats, Inbred Strains; D014605:Uveitis / Q000209:etiology
D000941:Antigens; D019393:Arrestin; D005136:Eye Proteins