title: Differentiation between Trichinella spiralis and T. pseudospiralis infective larvae by a monoclonal antibody.
abstract: Crude saline extracts of Trichinella spiralis and T. pseudospiralis infective larvae were studied by Western blot analysis using a monoclonal antibody, named ES/TA2 and produced against T. spiralis larvae. This monoclonal antibody recognized seven major antigenic components in T. spiralis larvae with apparent Mr: 45, 48, 50, 68, 70, 92 and 105 kDa and five in T. pseudospiralis larvae: 38, 50, 70, 72 and 92 kDa. SDS-PAGE of both extracts did not reveal appreciable differences in the range of molecular weights recognized by ES/TA2. These facts show the existence of immunological differences among proteins with apparently identical molecular weights.
authors.affiliation: Instituto López-Neyra de Parasitología, Unidad de Inmunología, Granada, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Rodriguez-Perez
authors.forename: V
authors.initials: V
authors.lastname: Gomez-Garcia
authors.forename: M
authors.initials: M
authors.lastname: Rodriguez-Osorio
authors.forename: J
authors.initials: J
authors.lastname: Rojas-Gonzalez
authors.forename: M A
authors.initials: MA
authors.lastname: Gomez-Morales
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000947:Antigens, Helminth / Q000032:analysis*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D007814:Larva / Q000276:immunology / Q000302:isolation & purification; D008970:Molecular Weight; D014234:Trichinella / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth