Stabilization by host cell components and Mg2+ of the neutralization epitopes of poliovirus.
When type 1 poliovirions were extracted from infected HeLa cells and heated at 56 degrees C in the presence of 1 mM-Mg2+, RNA-free, pluri-antigenic particles were formed. These particles possessed neutralization epitopes as well as epitopes associated with H antigen and with the isolated capsid protein VP3. The heating caused the formation of exclusively H antigenic particles when the Mg2+ concentration was 10(-4) M or lower, or when the virus was further purified in order to remove remaining cellular components. The ability of purified virus to form pluri-antigenic particles upon heating could be restored by incubation at 37 degrees C with infected cell extract.
Rombaut
B
B
Vrijsen
R
R
Boeyé
A
A
D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D000939:Epitopes / Q000276:immunology*; D006367:HeLa Cells / Q000382:microbiology; D006358:Hot Temperature; D006801:Humans; D008274:Magnesium / Q000494:pharmacology*; D009500:Neutralization Tests; D017955:Poliovirus / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology; D015678:Viral Structural Proteins; D014771:Virion / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D014764:Viral Proteins; D015678:Viral Structural Proteins; D008274:Magnesium