title: Identification and subcellular localization of a putative cell-to-cell transport protein from red clover mottle virus.
abstract: To investigate the mode of gene expression of red clover mottle virus (RCMV) middle component (M) RNA, we have synthesized an oligopeptide corresponding to the predicted carboxy-terminus of the RCMV counterparts of the cowpea mosaic virus (CPMV) 48K and 58K proteins. Using an antiserum raised against this synthetic oligopeptide, we have detected a 43-kDa protein in the 30,000 g pellet from extracts of RCMV-infected cowpea protoplasts. Immunogold cytochemistry further localized this protein to the plasmodesmata of RCMV-infected pea tissue. This subcellular location, taken together with other evidence, suggests that this 43-kDa protein has a role in the cell-to-cell spread of RCMV.
authors.affiliation: John Innes Institute, Norwich, United Kingdom.
authors.forename: M
authors.initials: M
authors.lastname: Shanks
authors.forename: K
authors.initials: K
authors.lastname: Tomenius
authors.forename: D
authors.initials: D
authors.lastname: Clapham
authors.forename: N S
authors.initials: NS
authors.lastname: Huskisson
authors.forename: P J
authors.initials: PJ
authors.lastname: Barker
authors.forename: I G
authors.initials: IG
authors.lastname: Wilson
authors.forename: A J
authors.initials: AJ
authors.lastname: Maule
authors.forename: G P
authors.initials: GP
authors.lastname: Lomonossoff
mesh_terms: D000918:Antibody Specificity; D015153:Blotting, Western; D015967:Gene Expression Regulation, Viral; D007106:Immune Sera / Q000276:immunology; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D009841:Oligonucleotides / Q000276:immunology; D010942:Plant Viruses / Q000032:analysis* / Q000235:genetics / Q000648:ultrastructure; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000235:genetics; D014764:Viral Proteins / Q000032:analysis* / Q000235:genetics
chemical_list: D007106:Immune Sera; D009841:Oligonucleotides; D012367:RNA, Viral; D014764:Viral Proteins