Amino acid sequence of a neutral phospholipase A2 (III) in the venom of Bungarus fasciatus.
Two phospholipases were found in the venom of Bungarus fasciatus, one in fraction III, the other in fraction X of the chromatographic separation. A neutral PLA2(III) purified from fraction III was subjected to amino acid sequencing by means of an automated sequenator applied to the intact RCM-PLA2 (III) and the individual peptides obtained from HPLC separation of the three types of enzymatic peptides. PLA(III) was shown to consist of 118 amino acid residues with 14 half-cystines. It is 65% homologous to the basic PLA2 obtained from fraction X.
Liu
C S
CS
Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan, Republic of China.
Leu
H L
HL
Chang
C S
CS
Chen
S W
SW
Lo
T B
TB
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004546:Elapid Venoms / Q000032:analysis*; D054501:Group II Phospholipases A2; D008969:Molecular Sequence Data; D010740:Phospholipases / Q000032:analysis*; D010741:Phospholipases A / Q000032:analysis*; D054467:Phospholipases A2; D030162:Reptilian Proteins; D012910:Snake Venoms
D004546:Elapid Venoms; D030162:Reptilian Proteins; D012910:Snake Venoms; D010740:Phospholipases; D010741:Phospholipases A; D054501:Group II Phospholipases A2; D054467:Phospholipases A2; C484164:phospholipase A2 III protein, Colubroidea