title: Peculiar features of application of pH gradients formed in borate buffer with a polyhydroxy compound for separation of proteins in a free-flow electrophoretic apparatus.
abstract: The use of pH gradients in borate buffer with a polyhydroxy compound for separation of proteins in a free-flow electrophoretic apparatus is outlined. The composition of the pH gradients, the free-flow apparatus design and its operation, and the main causes of failures in the protein separation are considered.
authors.affiliation: Institute of Immunology, Ministry of Medical and Microbiological Industry, Lyubuchany, Moscow Region, Chekhov District, U.S.S.R.
authors.forename: S A
authors.initials: SA
authors.lastname: Shukun
authors.forename: V P
authors.initials: VP
authors.lastname: Zav'yalov
mesh_terms: D001881:Borates; D002021:Buffers; D004586:Electrophoresis / Q000295:instrumentation*; D006863:Hydrogen-Ion Concentration; D011506:Proteins / Q000302:isolation & purification*
chemical_list: D001881:Borates; D002021:Buffers; D011506:Proteins