Monoclonal antibody-directed radioimmunoassay detects cytochrome P-450 in human placenta and lymphocytes.
A multiplicity of cytochromes P-450 is responsible for the detoxification and activation of xenobiotics such as drugs and carcinogens. Individual differences in sensitivity to these agents may reside in the cytochrome P-450 phenotype. A monoclonal antibody-directed radioimmunoassay was developed that detects epitope-specific cytochromes P-450 in human placentas and peripheral lymphocytes. Placentas from women who smoked cigarettes contained greater amounts of cytochrome P-450 with the monoclonal antibody-specific epitope than placentas from nonsmokers. The amount of this cytochrome P-450 in human peripheral lymphocytes increased after treatment of the mitogenized lymphocytes with the cytochrome P-450 inducer benz[a]anthracene.
Song
B J
BJ
Gelboin
H V
HV
Park
S S
SS
Tsokos
G C
GC
Friedman
F K
FK
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism; D001551:Benz(a)Anthracenes / Q000494:pharmacology; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis* / Q000276:immunology; D004790:Enzyme Induction / Q000187:drug effects; D000939:Epitopes / Q000276:immunology; D006801:Humans; D008214:Lymphocytes / Q000201:enzymology*; D008748:Methylcholanthrene / Q000494:pharmacology; D008861:Microsomes / Q000201:enzymology; D008862:Microsomes, Liver / Q000201:enzymology; D010920:Placenta / Q000201:enzymology*; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats
D000911:Antibodies, Monoclonal; D001551:Benz(a)Anthracenes; D000939:Epitopes; D008748:Methylcholanthrene; D003577:Cytochrome P-450 Enzyme System; C030935:benz(a)anthracene; D001189:Aryl Hydrocarbon Hydroxylases