Characterization of the high pH wobble structure of the 2-aminopurine.cytosine mismatch by N-15 NMR spectroscopy.
Transition mutations induced by the base analogue 2-aminopurine arise via the formation of AP.C base pairs during DNA replication. We report here the results of N-15 NMR studies on a duplex oligonucleotide containing N-15 enriched AP and C residues. At high pH (8.6) the AP.C base pair is predominantly wobble. This is the first report on use of a site specifically N-15 enriched oligonucleotide as a probe of aberrant base pairing in DNA.
Sowers
L C
LC
Division of Pediatrics, City of Hope National Medical Center, Duarte, CA.
Eritja
R
R
Chen
F M
FM
Khwaja
T
T
Kaplan
B E
BE
Goodman
M F
MF
Fazakerley
G V
GV
D015075:2-Aminopurine; D000225:Adenine / Q000031:analogs & derivatives; D001482:Base Composition; D003596:Cytosine; D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009587:Nitrogen Isotopes; D009690:Nucleic Acid Conformation
D009587:Nitrogen Isotopes; D015075:2-Aminopurine; D003596:Cytosine; D000225:Adenine