Kinetics and quantitation of In-111 labeled platelet deposition on control and heparin-bonded polyurethane angio catheters in a dog model.
The dynamics of platelet deposition on control polyurethane catheters (CPC) and heparin-bonded polyurethane catheters (HBPC) were evaluated with In-111 labeled platelets (In-PLT) using a computerized gamma camera (CGC). Ten nonheparinized dogs (18-25 kg) had both femoral arteries catherized with 10 cm of CPC and HBPC (5 Fr.) 24 hr postinjection of 300-420 microcuries of In-PLT, and imaged for 3 hr with a gamma camera. Regional platelet deposition on three segments of catheters and the puncture site was determined. Catheters were harvested and radioactivity on the catheter segments (proximal: PROX, middle: MID, distal: DIST and puncture site: PS) of both was determined. From the platelet count in blood, and radioactivity in blood and segments of catheters, adjacent artery, and area of artery and catheter, the platelet-density [X10(3) (mean +/- S.D.)] on catheter and artery was calculated and tabulated. Proximal values were cath (CPC), 1289 +/- 1125; artery, 1355 +/- 587; cath (HBPC), 125 +/- 113; artery, 1149 +/- 1620. The middle values were cath (CPC), 1102 +/- 1109; artery, 1512 +/- 625; cath (HBPC), 132 +/- 108; artery, 1011 +/- 942. Distal values were cath (CPC), 780 +/- 584; artery, 132 +/- 108; cath (HBPC), 227 +/- 194; artery, 1457 +/- 1309. The puncture site values were cath (CPC), 106 +/- 382; artery, 1011 +/- 942; cath (HBPC), 164 +/- 135; artery, 1498 +/- 1240. The large standard deviation in retained platelets is due to embolization. The platelet-density and regional counts on catheter segments were lower with HBPC than CPC, as was the rate of platelet-deposition.(ABSTRACT TRUNCATED AT 250 WORDS)
Dewanjee
M K
MK
University of Miami, School of Medicine, Department of Radiology, Florida 33101.
Rowland
S M
SM
Robinson
R P
RP
Dewanjee
P K
PK
Halgovich
J L
JL
Kapadvanjwala
M
M
MacGregor
D C
DC
Serafini
A N
AN
Palatianos
G M
GM
Sfakianakis
G N
GN
D000792:Angiography / Q000295:instrumentation*; D000818:Animals; D001792:Blood Platelets / Q000502:physiology*; D002408:Catheters, Indwelling; D004285:Dogs; D006493:Heparin / Q000008:administration & dosage*; D007205:Indium Radioisotopes; D010973:Platelet Adhesiveness / Q000187:drug effects / Q000502:physiology*; D010974:Platelet Aggregation / Q000502:physiology; D011140:Polyurethanes; D011877:Radionuclide Imaging; D013927:Thrombosis / Q000000981:diagnostic imaging
D007205:Indium Radioisotopes; D011140:Polyurethanes; D006493:Heparin