Voltage-dependent removal of sodium inactivation by N-bromoacetamide and pronase.
When perfused internally through crayfish giant axons, pronase removed sodium inactivation more than three times as fast at -100 mV as compared with -30 mV. N-bromoacetamide, applied internally, removed sodium inactivation twice as fast at -100 mV as at -30 mV, and the relative rate of removal declined with membrane depolarization in proportion to steady-state sodium inactivation. We conclude that in the closed conformation the sodium inactivation gate is partially protected from destruction by N-bromoacetamide and pronase.
Salgado
V L
VL
Yeh
J Z
JZ
Narahashi
T
T
D000081:Acetamides / Q000494:pharmacology*; D000818:Animals; D003400:Astacoidea; D001369:Axons / Q000502:physiology*; D004558:Electric Stimulation; D007473:Ion Channels / Q000187:drug effects* / Q000502:physiology; D008564:Membrane Potentials; D011402:Pronase / Q000494:pharmacology*; D012964:Sodium / Q000378:metabolism*
D000081:Acetamides; D007473:Ion Channels; C016272:N-bromoacetamide; D012964:Sodium; D011402:Pronase