title: Surfactant enhancement of polyethyleneglycol-induced cell fusion.
abstract: B16 mouse melanoma cells in monolayers may be satisfactorily fused with 50% PEG 1500. However, pre-treatment with detergents in solution at low concentrations significantly increases PEG fusion, up to 8-fold in some instances, without impairing cell viability. The practical and mechanistical implications of this finding are discussed.
authors.affiliation: Department of Cell Biology, University of Basque Country, Bilbao, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Prado
authors.forename: M A
authors.initials: MA
authors.lastname: Partearroyo
authors.forename: M
authors.initials: M
authors.lastname: Mencía
authors.forename: F M
authors.initials: FM
authors.lastname: Goñi
authors.forename: E
authors.initials: E
authors.lastname: Barberá-Guillem
mesh_terms: D000818:Animals; D002459:Cell Fusion / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D003902:Detergents / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D051379:Mice; D011092:Polyethylene Glycols / Q000494:pharmacology*; D013501:Surface-Active Agents / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D003902:Detergents; D013501:Surface-Active Agents; D011092:Polyethylene Glycols