Glycoprotein PAS-0 from the milk fat globule membrane carries antigenic determinants for epithelial membrane antigen.
Epithelial membrane antigen (EMA) can be found in human tissues using antisera raised against defatted human cream. PAS-0 is a glycoprotein which has been extracted from human milk fat globule membranes. Using a polyclonal antiserum and a series of monoclonal antibodies, we have shown that the major antigenic determinant for EMA is carried by PAS-0. A more detailed comparison of the two glycoproteins has been made by establishing a set of radioimmunoassays using the different antibodies.
Ormerod
M G
MG
McIlhinney
J
J
Steele
K
K
Shimizu
M
M
D000596:Amino Acids / Q000032:analysis; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000276:immunology*; D002241:Carbohydrates / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis*; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D008565:Membrane Proteins / Q000276:immunology*; D018396:Mucin-1; D011863:Radioimmunoassay
D000596:Amino Acids; D000941:Antigens; D002241:Carbohydrates; D000939:Epitopes; D006023:Glycoproteins; D008565:Membrane Proteins; D018396:Mucin-1