Biosynthesis of small proteoglycans by hepatic lipocytes in primary culture.
Chondroitin sulfate/dermatan sulfate proteoglycans were obtained from the secretions of cultured rat hepatic lipocytes. The collagen-binding small proteoglycan II represented only a minor species (less than 10%), whereas similar amounts of small proteoglycan I and of a novel collagen-binding proteoglycan with a core protein of 101 kDa were found. These results support the concept of a special role of lipocytes in the pathogenesis of liver fibrosis.
Witsch
P
P
Institute of Physiological Chemistry and Pathobiochemistry, University of Münster, FRG.
Kresse
H
H
Gressner
A M
AM
D000818:Animals; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D007930:Leucine / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008970:Molecular Weight; D011509:Proteoglycans / Q000096:biosynthesis* / Q000302:isolation & purification; D051381:Rats; D011919:Rats, Inbred Strains; D013431:Sulfates / Q000378:metabolism; D013462:Sulfur Radioisotopes; D014316:Tritium
D011509:Proteoglycans; D013431:Sulfates; D013462:Sulfur Radioisotopes; D014316:Tritium; D007930:Leucine