title: Expression of srnB gene of F plasmid by altered RNA polymerase in Escherichia coli.
abstract: Degradation of otherwise stable rRNA and tRNA takes place in the presence of rifampin, dependent on the F plasmid srnB gene. We have reported that a protein newly synthesized in the presence of rifampin might be a product of the srnB gene required for stable RNA degradation (Ito, R. and Ohnishi, Y. (1983) Biochim. Biophys. Acta 739, 27-34). Here we have further studied the mechanism of srnB expression. Among eighteen mutants with altered RNA polymerase, two (TJ2470 (rpoC4) and TJ302 (rpoC56)) showed RNA degradation at high temperature (42 degrees C) when the srnB gene was present. Labeling proteins at 42 degrees C in strain TJ2470 indicated that a protein of molecular weight 12 000 was a product of the srnB gene, and that expression of the srnB gene provoked RNA degradation. Using plasmid pTK4, in which the srnB gene is inserted downstream of the promoter of lacZ, lac promoter-dependent expression of the srnB gene, with production of the putative protein product, also induced RNA degradation at 42 degrees C, with no requirement for added rifampin or altered RNA polymerase. RNA degradation in these conditions was quite similar to that in the case of the addition of rifampin; e.g., it showed some responses to Mg2+, temperature and RNAase I content of the cells. Expression of the srnB gene dependent on lac promoter was also observed in minicells. Thus, it is inferred that the srnB gene is probably repressed under normal conditions with its own promoter; its expression initiates RNA turnover.
authors.forename: R
authors.initials: R
authors.lastname: Ito
authors.forename: S
authors.initials: S
authors.lastname: Akimoto
authors.forename: Y
authors.initials: Y
authors.lastname: Ohnishi
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D004262:DNA Restriction Enzymes; D012321:DNA-Directed RNA Polymerases / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005144:F Factor; D005796:Genes; D005798:Genes, Bacterial; D007700:Kinetics; D007763:Lac Operon; D009154:Mutation; D012329:RNA, Bacterial / Q000378:metabolism; D013045:Species Specificity; D014158:Transcription, Genetic; D014169:Transformation, Bacterial
chemical_list: D001426:Bacterial Proteins; D012329:RNA, Bacterial; D012321:DNA-Directed RNA Polymerases; D004262:DNA Restriction Enzymes