title: The whole blood oxygen affinity in normal human newborns: II) P50, n value and related parameters.
abstract: The cord blood from 82 normal newborns (48 males and 34 females) has been extensively investigated, to check p50, cooperativity and related parameters. The most important results are the following: Neonatal p50 is slightly above 20 torr, in good agreement with the literature data, but this value refers to the p50 "CO-free" (T50); otherwise, the original p50 is slightly less than 22 torr, a value which is not indicative enough, because neonatal blood contains a consistent excess of Hb-CO. Either this CO-excess and the low p50 value seem to be mainly depending on the low binding capacity of Hb-F with DPG, even if further studies are still necessary at regard. The empirical factor correcting adult p50 to T50 is -0.27, a value which must be increased to -0.36 for neonatal blood. Multiple gas analyses of the samples indicate that oxygen transport is a very complicate equilibrium involving pH, CO2, bicarbonate balance and other factors. A complete statistical analysis of the data has been carried out and the main result indicates that oxygen assumption favours CO2 releasing and vice versa, a phenomenon already well known and globally called "Bohr effect".
authors.forename: F
authors.initials: F
authors.lastname: Tosetti
authors.forename: C
authors.initials: C
authors.lastname: Fabris
authors.forename: C
authors.initials: C
authors.lastname: Oneglia
authors.forename: P G
authors.initials: PG
authors.lastname: Pich
authors.forename: M F
authors.initials: MF
authors.lastname: Bertero
authors.forename: E
authors.initials: E
authors.lastname: Rabino-Massa
authors.forename: U
authors.initials: U
authors.lastname: Marchisio
authors.forename: P
authors.initials: P
authors.lastname: Burzio
authors.forename: G
authors.initials: G
authors.lastname: Ricco
mesh_terms: D001639:Bicarbonates / Q000097:blood; D002245:Carbon Dioxide / Q000097:blood; D005260:Female; D005312:Fetal Blood / Q000378:metabolism*; D005319:Fetal Hemoglobin / Q000032:analysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007231:Infant, Newborn; D008297:Male; D010100:Oxygen / Q000097:blood*; D012016:Reference Values; D012737:Sex Factors
chemical_list: D001639:Bicarbonates; D002245:Carbon Dioxide; D005319:Fetal Hemoglobin; D010100:Oxygen