Hemagglutination assay for human serum fibronectin.
A hemagglutination assay for human serum fibronectin (Fn) is described. The assay is based on the capacity of Fn to bind to gelatin. Purified and human serum Fn agglutinate chromic chloride treated sheep red cells coated with gelatin in a quantitative and reproducible manner. The lowest Fn concentration giving positive hemagglutination was found to be 7 micrograms/ml, a sensitivity making it suitable for measurement of Fn in both normal and pathologic sera. The assay is simple and rapid and does not require specialized equipment.
Bălună
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Barac
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Tarnavschi
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Belaşcu
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D000818:Animals; D000939:Epitopes; D004912:Erythrocytes / Q000276:immunology; D005353:Fibronectins / Q000097:blood* / Q000378:metabolism; D005780:Gelatin / Q000378:metabolism; D006386:Hemagglutination Tests / Q000379:methods*; D006801:Humans; D012756:Sheep / Q000276:immunology
D000939:Epitopes; D005353:Fibronectins; D005780:Gelatin