BKm minisatellite sequences are not sex associated but reveal DNA fingerprint polymorphisms in rainbow trout.
GATA-GACA repetitive sequences first isolated from a female snake (termed BKm sequences) and associated with sex chromosomes in some species were hybridized to DNA from rainbow trout (Salmo gairdneri). Genomic DNA was studied from three groups of rainbow trout: (i) randomly selected males and females from an outbred group, (ii) androgenetic individuals from an inbred strain, and (iii) parents and offspring of an outbred strain. Three restriction enzymes (EcoRI, HaeIII, or HinfI) were used to digest the genomic DNA. The DNA was electrophoresed in agarose gels, transferred to nylon membranes, and the GATA-GACA repetitive sequence probe was hybridized to this DNA. There was no evidence of sex-associated patterns of hybridization with the enzymes used. However, the sequences reveal DNA fingerprint polymorphisms which appear to be inherited in a stable manner.
Lloyd
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MA
Program in Genetics and Cell Biology, Washington State University, Pullman 99164-4350.
Fields
M J
MJ
Thorgaard
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GH
D000818:Animals; D015139:Blotting, Southern; D003433:Crosses, Genetic; D004276:DNA, Satellite / Q000235:genetics*; D005260:Female; D008297:Male; D009710:Nucleotide Mapping; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid; D012487:Salmonidae / Q000235:genetics*; D012727:Sex Characteristics; D014337:Trout / Q000235:genetics*; D014960:X Chromosome; D014998:Y Chromosome
D004276:DNA, Satellite