[Inhibitory effect of human alpha 2-macroglobulin and a peptide released by trypsin, on the G1-S transition of hepatocytes in vivo].
Highly purified human alpha 2 M inhibits hepatocyte proliferation. 1 mg of alpha 2 M corresponds to 1 baby rat unit (BRU). alpha 2 M is bound to a low molecular weight glycopeptide, which is released during trypsinization of alpha 2 M. 3 micrograms of trypsin-treated alpha 2 M release 1 BRU. alpha 2 M and the glycopeptide have been shown to be identical, respectively, to high and low molecular weight components present in normal human plasma. Both components inhibit the G1-S transition of the hepatocyte cycle. alpha 2 M acts as an antagonist to the inhibitory effect of the glycopeptide when the molar ratio of trypsin to alpha 2 M is greater than 2.
Boffa
G A
GA
Lambin
P
P
Rius
E
E
Nadal
C
C
D000328:Adult; D000818:Animals; D006020:Glycopeptides / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D007399:Interphase / Q000187:drug effects; D008099:Liver / Q000166:cytology* / Q000187:drug effects; D051381:Rats; D014357:Trypsin / Q000378:metabolism; D000511:alpha-Macroglobulins / Q000378:metabolism / Q000494:pharmacology*
D006020:Glycopeptides; D000511:alpha-Macroglobulins; D014357:Trypsin
Effet inhibiteur de l'alpha 2 macroglobuline humaine et d'un peptide libéré par la trypsine, sur la transition G1-S des hépatocytes, in vivo.