Radioimmunoassay of human platelet-derived growth factor using monoclonal antibody toward a synthetic 73-97 fragment of its B-chain.
A mouse monoclonal antibody toward a 73-97 fragment of human platelet-derived growth factor (hPDGF) B-chain was used to develop a radioimmunoassay (RIA) for serum hPDGF. By the single step procedure of the double antibody technique, the measurable range was 10-1,000 micrograms/l. The coefficients of variation within and between series were 10.2% and 12.1% respectively, and satisfactory dilution curves were obtained for sera from healthy subjects. The hPDGF levels in all plasma samples from 15 healthy subjects examined were below the detection limit (10 micrograms/l), whereas the mean hPDGF concentration (+/- SD) in serum samples of 60 healthy subjects was 31.9 +/- 20.4 micrograms/l. This value was significantly (p less than 0.01) higher than the mean for 21 patients with idiopathic thrombocytopenic purpura (12.6 +/- 4.5 micrograms/l). There was a significant positive (r = 0.481, p less than 0.01) but not a strong (r2 = 0.23) correlation between the peripheral blood platelet counts and serum hPDGF levels of all subjects. This RIA system should be useful clinically for measurement of serum hPDGF.
Shiraishi
T
T
Department of Geriatric Medicine, Osaka University Medical School, Japan.
Morimoto
S
S
Itoh
K
K
Sato
H
H
Sugihara
K
K
Onishi
T
T
Ogihara
T
T
D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D010446:Peptide Fragments / Q000276:immunology*; D010982:Platelet-Derived Growth Factor / Q000032:analysis* / Q000276:immunology; D011696:Purpura, Thrombocytopenic / Q000097:blood; D011863:Radioimmunoassay / Q000592:standards; D012016:Reference Values
D000911:Antibodies, Monoclonal; D010446:Peptide Fragments; D010982:Platelet-Derived Growth Factor