title: The Agrobacterium tumefaciens virC1 gene product binds to overdrive, a T-DNA transfer enhancer.
abstract: In Agrobacterium tumefaciens, a cis-active 24-base-pair sequence adjacent to the right border of the T-DNA, called overdrive, stimulates tumor formation by increasing the level of T-DNA processing. Recent results from our laboratory have suggested that the virC operon which enhances T-DNA processing probably does so because the VirC1 protein interacts with overdrive (N. Toro, A. Datta, M. Yanofsky, and E. W. Nester, Proc. Natl. Acad. Sci. USA 85:8558-8562, 1988). We report here the purification of the VirC1 protein from cells of Escherichia coli harboring a plasmid containing the coding sequences of the virC locus of the octopine Ti plasmid. By gel mobility shift and DNase I footprinting assays, we showed that this purified virC1 gene product binds to overdrive but not to the right border of T-DNA.
authors.affiliation: Department of Microbiology, University of Washington, Seattle 98195.
authors.forename: N
authors.initials: N
authors.lastname: Toro
authors.forename: A
authors.initials: A
authors.lastname: Datta
authors.forename: O A
authors.initials: OA
authors.lastname: Carmi
authors.forename: C
authors.initials: C
authors.lastname: Young
authors.forename: R K
authors.initials: RK
authors.lastname: Prusti
authors.forename: E W
authors.initials: EW
authors.lastname: Nester
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics* / Q000378:metabolism; D003850:Deoxyribonuclease I; D004742:Enhancer Elements, Genetic; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009710:Nucleotide Mapping; D015345:Oligonucleotide Probes; D011485:Protein Binding; D012231:Rhizobium / Q000235:genetics* / Q000378:metabolism; D037521:Virulence Factors
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D015345:Oligonucleotide Probes; D037521:Virulence Factors; D003850:Deoxyribonuclease I