[A study of monoclonal antibodies to HBsAg by a method of radioimmunoassay with autoradiographic recording of results].
Radioimmunoassay (RIA) with autoradiographic registration of the results was used to study 11 monoclonal antibodies (MCA) to HBsAg. The MCA were highly specific, stable, had sufficiently high titres in the range of 4 X 10(4) to 6 X 10(5). They were divided into 3 competition groups; a correlation was established between the appurtenance of MCA to a certain competition group, their amount, titre, and activity as "catch" antibody. The a-HBs MCA proved to be of low effectiveness as iodinated indicators of immune complexes. The expedience of using a-HBs MCA and combinations thereof as "catch" antibodies in detection of HBsAg in sera of asymptomatic carriers was shown. The experimental data indicate the advantages of using the autoradiographic variant of RIA both for screening of MCA and for the development on the basis of a-HBs MCA of highly technologic and economic diagnostic systems for HBsAg detection.
Semenova
N P
NP
Prokudina
E N
EN
Karepina
M V
MV
Kushch
A A
AA
D000911:Antibodies, Monoclonal; D001345:Autoradiography; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D006514:Hepatitis B Surface Antigens
Issledovanie monoklonal'nykh antitel k HBsAg metodom radioimmunologicheskogo analiza s avtoradiograficheskoĭ registratsieĭ rezul'tatov.