Homologies between paraflagellar rod proteins from trypanosomes and euglenoids revealed by a monoclonal antibody.
A Nonidet P 40 insoluble fraction was isolated from Trypanosoma brucei and was used to raise a monoclonal antibody (5E9). The antigen was localized by indirect immunofluorescence in the flagellum of T. brucei and of two species of euglenoids, Euglena gracilis and Distigma proteus. In immunoblot analysis, 5E9 appeared to bind to paraflagellar rod proteins PFR1 and PFR2 of T. brucei (72000 and 75000 mol. wt.) and of E. gracilis (67000 and 76000 mol. wt.). The presence of a common epitope in paraflagellar rod proteins from species of trypanosomes and euglenoids shows that despite distinct structures of the rods some identical domain exists in the proteins that could be involved in their supramolecular assembly into a similar organelle. The antigenic determinant defined by 5E9 was also shown to be present in a 87000 molecular weight polypeptide located in the proximal part of the flagellum of Crithidia oncopelti in which a paraflagellar rod is not detectable at the ultrastructural level.
Gallo
J M
JM
Schrével
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000953:Antigens, Protozoan / Q000276:immunology*; D003421:Crithidia / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D005056:Euglena gracilis / Q000276:immunology*; D005407:Flagella / Q000276:immunology; D007120:Immunochemistry; D008970:Molecular Weight; D015800:Protozoan Proteins; D013045:Species Specificity; D014346:Trypanosoma brucei brucei / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D000939:Epitopes; D015800:Protozoan Proteins; C045519:paraflagellar rod proteins, Trypanosoma brucei