Basal-cell subpopulations and cell-cycle kinetics in human epidermal explant cultures.
Cultured human epidermal cells were studied by cell sorting and autoradiography after different 3H-thymidine (3H-dThd)-labelling procedures and after labelling with DNA precursors that are incorporated via salvage or de novo pathways. It was shown that 3H-dThd incorporation was the best measure of the rate of DNA replication. Dose-response experiments with pulse and continuous labelling revealed that all S- and G2-phase cells were cycling, whereas some 20% of the cells stayed in G1-phase for long periods of time. Most, if not all of these cells were probably non-proliferating differentiated keratinocytes. At least two subpopulations of S-phase cells could be discriminated on the basis of the rate of incorporation of DNA precursors. The difference in precursor incorporation did not seem to be caused by differences in nucleotide metabolism but rather to reflect true differences in the rate of DNA replication. Continuous labelling experiments showed that these subpopulations also were apparent in the G1- and G2-phases. Studies of the grain-count distribution revealed that cells that appeared to move rapidly through the S-phase moved slowly through the G2-phase, and vice versa. Cells stained with acridine orange were subjected to a two-parameter analysis in the cell sorter by simultaneous measurement of the DNA and RNA fluorescence. Autoradiography of sorted cells revealed that, on average, cells with low RNA contents incorporated 3H-dThd at a higher rate than cells with high RNA contents.
Jensen
P K
PK
Pedersen
S
S
Bolund
L
L
D000165:Acridine Orange; D000328:Adult; D001345:Autoradiography; D002453:Cell Cycle; D002469:Cell Separation; D002477:Cells / Q000145:classification; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006801:Humans; D007399:Interphase; D007700:Kinetics; D012313:RNA / Q000378:metabolism; D012867:Skin / Q000166:cytology* / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D014316:Tritium; D012313:RNA; D004247:DNA; D000165:Acridine Orange; D013936:Thymidine