Characterization of metalloproteinases in rat gastric tissues with acetic acid-induced ulcers.
To clarify the destruction of connective tissue in ulcerated regions, collagenase and gelatinolytic activities in homogenates of rat acetic acid-induced ulcers were examined. Gelatinolytic activity in the ulcerated regions was significantly higher than that in normal tissues. Collagenase, however, was not detectable. Gelatin-gel-electrophoresis showed that the gelatinolytic activity was due to several species, some of which crossreacted with a sheep anti-(rabbit prostromelysin) antibody. The H2-blocker, famotidine, significantly depressed the gelatinolytic activity in the ulcerated regions. Thus, both stromelysin and gelatinolytic enzymes may play important roles in the degradation of the basement membrane, especially type IV collagen.
Ito
A
A
Department of Biochemistry, Tokyo College of Pharmacy, Japan.
Nagase
H
H
Mori
Y
Y
D000085:Acetates; D019342:Acetic Acid; D000818:Animals; D004792:Enzyme Precursors / Q000378:metabolism; D015738:Famotidine / Q000494:pharmacology; D005753:Gastric Mucosa / Q000378:metabolism; D005780:Gelatin / Q000378:metabolism; D008297:Male; D008666:Metalloendopeptidases / Q000145:classification* / Q000378:metabolism; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains; D013270:Stomach / Q000201:enzymology*; D013276:Stomach Ulcer / Q000139:chemically induced / Q000201:enzymology* / Q000378:metabolism; D013997:Time Factors
D000085:Acetates; D004792:Enzyme Precursors; D015738:Famotidine; D005780:Gelatin; D008666:Metalloendopeptidases; D019342:Acetic Acid