Argentaffin and argyrophil reactions and serotonin content of endocrine tumours.
Sixty carcinoid tumours were tested in a retrospective study with an immunoperoxidase technique using a monoclonal antibody against serotonin immunoreactive sites, with argyrophil staining using the Grimelius technique, and with argentaffin staining using the Masson-Fontana technique. A good correlation between all three techniques in the diagnosis of ileal carcinoid tumour was found, but the immunoperoxidase technique showed greater sensitivity than the Masson-Fontana technique and greater specificity than the Grimelius technique in the diagnosis of foregut and hindgut carcinoid tumours. The immunoperoxidase technique with a monoclonal antibody against serotonin immunoreactive sites (YC5/45) is recommended as a sensitive and specific test for carcinoid tumours. The reactions in other endocrine tumours are also included.
Wells
C A
CA
Taylor
S M
SM
Cuello
A C
AC
D000911:Antibodies, Monoclonal / Q000276:immunology; D002276:Carcinoid Tumor / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007078:Ileal Neoplasms / Q000473:pathology; D007082:Ileum / Q000473:pathology; D007124:Immunoenzyme Techniques; D007413:Intestinal Mucosa / Q000473:pathology; D012189:Retrospective Studies; D012701:Serotonin / Q000276:immunology / Q000378:metabolism*; D012834:Silver; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal; D012701:Serotonin; D012834:Silver