The putative oncogene Spi-1: murine chromosomal localization and transcriptional activation in murine acute erythroleukemias.
We have shown previously that spleen focus forming virus integration near the Spi-1 putative oncogene is observed in 95% of erythroid Friend tumors (Moreau-Gachelin et al., 1988). Here we describe how the proviral insertion in the Spi-1 domain is associated with the enhanced transcription of a 1.4 kb mRNA normally expressed at a low level in normal cells. The gene is localized on murine chromosome 2, band E3. The structure of the Spi-1 gene was determined by sequencing genomic and cDNA clones. The gene has an open reading frame encoding a protein of 218 amino acids, extending over five exons. Proviruses always integrate outside, upstream and in the opposite orientation of the protein-encoding domain. This suggests that SFFV integration activates the expression of Spi-2 gene that may contribute to the erythroleukemic process.
Moreau-Gachelin
F
F
INSERM U-248, Faculté de Médecine Lariboisière Saint Louis, Paris, France.
Ray
D
D
Mattei
M G
MG
Tambourin
P
P
Tavitian
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002874:Chromosome Mapping; D004268:DNA-Binding Proteins; D005622:Friend murine leukemia virus / Q000235:genetics; D015698:Genomic Library; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics*; D007942:Leukemia, Experimental / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D009857:Oncogenes; D012326:RNA Splicing; D015183:Restriction Mapping; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics*; D014158:Transcription, Genetic; D015533:Transcriptional Activation
D004268:DNA-Binding Proteins; D015682:Retroviridae Proteins, Oncogenic; C062022:v-Spi-1 protein, Friend spleen focus-forming virus