Life span of human corneal endothelial cells in long-term cultures.
Human corneal endothelial cells (HCEC) were isolated from excised corneas of adult donors in the age range of 28-70 years, and successfully grown for 10-12 passages. Moreover, one cell line, that had been established from a 45-year-old donor, was further passaged at defined split ratios in order to determine the proliferative life span of the HCEC isolated from donors of this age group.
Engelmann
K
K
Abteilung Cytogenetik, Gesellschaft für Biotechnologische Forschung mbH, Braunschweig; BRD.
Böhnke
M
M
Friedl
P
P
D000328:Adult; D000368:Aged; D002455:Cell Division; D002470:Cell Survival; D002478:Cells, Cultured; D003470:Culture Media; D004728:Endothelium, Corneal / Q000166:cytology*; D006801:Humans; D007621:Karyotyping; D008875:Middle Aged; D013997:Time Factors
D003470:Culture Media