title: Rates of mutation to growth factor autonomy and tumorigenicity differ in hematopoietic stem and precursor cells expressing the multilineage colony-stimulating factor gene.
abstract: At least two separate but interdependent events are required to attain autonomous growth as a consequence of ectopic expression of the multilineage colony-stimulating factor gene in hematopoietic progenitor cells. The rate at which the second event occurs is more than 3 orders of magnitude higher in precursor cell lines (FDC-P1 or FDC-P2) than in stem cell lines (FDC-Pmix). Autonomous, but not density-dependent, growth is tightly coupled to tumorigenicity in precursor cells; however, neither growth-factor-independent nor autonomously growing stem cell lines are tumorigenic.
authors.affiliation: Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Universität Hamburg, Federal Republic of Germany.
authors.forename: C
authors.initials: C
authors.lastname: Laker
authors.forename: N
authors.initials: N
authors.lastname: Kluge
authors.forename: C
authors.initials: C
authors.lastname: Stocking
authors.forename: U
authors.initials: U
authors.lastname: Just
authors.forename: M J
authors.initials: MJ
authors.lastname: Franz
authors.forename: W
authors.initials: W
authors.lastname: Ostertag
authors.forename: J F
authors.initials: JF
authors.lastname: DeLamarter
authors.forename: M
authors.initials: M
authors.lastname: Dexter
authors.forename: E
authors.initials: E
authors.lastname: Spooncer
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D003115:Colony-Stimulating Factors / Q000235:genetics* / Q000276:immunology / Q000494:pharmacology; D015870:Gene Expression; D005822:Genetic Vectors; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D007106:Immune Sera; D007700:Kinetics; D051379:Mice; D009154:Mutation
chemical_list: D003115:Colony-Stimulating Factors; D007106:Immune Sera