Reversible acetaldehyde inhibition of A23187-stimulated amylase secretion from isolated rat pancreatic acini.
The Ca2+ ionophore, A23187, stimulated amylase secretion from isolated rat pancreatic acini in a dose-dependent manner with a maximal effect at 6 microM. Acetaldehyde, a metabolite of ethanol, caused a reduction in the magnitude of ionophore-stimulated secretion with no evidence of competitive inhibition. Furthermore, 6 microM ionophore-stimulated amylase secretion was dose-dependently inhibited by acetaldehyde. This inhibitory effect of acetaldehyde, however, was reversible on washing and reincubating acetaldehyde-treated acini. These results suggest that acetaldehyde reversibly inhibits intracellular components mediating stimulated secretion and this inhibition requires a continuous chemical interaction between acetaldehyde and intracellular component(s) regulating stimulated enzyme secretion.
Lewin
M B
MB
Wong
A
A
Deveney
C W
CW
Sankaran
H
H
D000079:Acetaldehyde / Q000494:pharmacology*; D000681:Amylases / Q000378:metabolism*; D000818:Animals; D000001:Calcimycin / Q000037:antagonists & inhibitors*; D002118:Calcium / Q000502:physiology; D005260:Female; D010179:Pancreas / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012636:Secretory Rate / Q000187:drug effects
D000001:Calcimycin; D000681:Amylases; D000079:Acetaldehyde; D002118:Calcium