A tissue-specific change in repetitive DNA in rats.
From a genomic library constructed from an EcoRI digest of Wistar rat brain DNA, we isolated a clone (BL-1) that gave a 0.6-kilobase restriction fragment only in brain (or lens) DNA upon Southern hybridization. The tissue-specific fragment was present in Wistar and other strains (Sprague-Dawley and Donryu) of rats regardless of their sex and age. Sequencing of the clone indicated that it is closely related to a part of the LINE 3 sequence, one of the highly repetitive sequences present throughout mammalian genomes. Polymerase chain reaction using primer sequences in the BL-1 clone indicated that it is derived from an amplified (rear-ranged) sequence, although other explanations are possible. These results suggest that there are tissue-specific changes in DNA primary structure during mammalian developmental processes.
Yokota
H
H
Furusawa Morpho Gene Project, Research Development Corporation of Japan, Ibaragi.
Iwasaki
T
T
Takahashi
M
M
Oishi
M
M
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D001921:Brain / Q000378:metabolism; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004259:DNA-Directed DNA Polymerase; D015246:Deoxyribonuclease EcoRI; D005260:Female; D015698:Genomic Library; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism; D021141:Nucleic Acid Amplification Techniques; D009693:Nucleic Acid Hybridization; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D051381:Rats; D011919:Rats, Inbred Strains; D012091:Repetitive Sequences, Nucleic Acid
D004247:DNA; D004259:DNA-Directed DNA Polymerase; D015246:Deoxyribonuclease EcoRI