A quantitative explanation of the effects of some alcohols on gramicidin single-channel lifetime.
The effects of n-decanol, n-hexadecanol, n-octyl(oxyethylene)3 alcohol and cholesterol on gramicidin single-channel lifetime in planar lipid bilayers have been determined. The bilayers used were formed from a solution of monoolein in squalene. Measurements have also been made of the above compounds' effects on membrane thickness (as measured by electrical capacity and optical reflectance technique) and surface tension (as derived from bulk interfacial tension and bilayer-lens contact angle measurements). The reduction in single-channel lifetime caused by the n-alkanols may be accounted for quantitatively in terms of the effects of these compounds on bilayer thickness and surface tension. The n-octyl(oxyethylene)3 alcohol caused an increase in single-channel lifetime which is also consistent with the thickness/tension theory. The reduction in channel lifetime caused by cholesterol, however, was much larger than would be predicted from its effects on bilayer thickness and surface tension.
Elliott
J R
JR
Needham
D
D
Dilger
J P
JP
Brandt
O
O
Haydon
D A
DA
D000438:Alcohols / Q000494:pharmacology*; D002784:Cholesterol / Q000494:pharmacology; D005233:Fatty Alcohols / Q000494:pharmacology; D006096:Gramicidin / Q000378:metabolism*; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism*; D008051:Lipid Bilayers / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000494:pharmacology; D013500:Surface Tension
D000438:Alcohols; D005233:Fatty Alcohols; D007473:Ion Channels; D008051:Lipid Bilayers; D006096:Gramicidin; C045068:n-octyl(oxyethylene)(3)ethanol; D011092:Polyethylene Glycols; C029383:n-decyl alcohol; C005031:cetyl alcohol; D002784:Cholesterol