Molecular cloning of the Rauscher murine leukemia virus. Disease spectrum and integration pattern.
After transfection of NIH 3T3 cells with DNA from molecularly cloned Rauscher MuLV, virus was isolated which showed a disease spectrum comparable to that of R-MuLV cloned biologically by endpoint dilution. In both cases sites of proviral integration vary from 2-5 per leukemic tissue and occur apparently at random.
van der Feltz
M J
MJ
Department of Pathology, Faculty of Medicine, Erasmus University Rotterdam, The Netherlands.
Kranendonk-Odijk
M E
ME
Rhijnsburger
E H
EH
Stark
J
J
Stoker
K
K
de Both
N J
NJ
D000818:Animals; D015139:Blotting, Southern; D002460:Cell Line; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D007942:Leukemia, Experimental / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D011533:Proviruses / Q000235:genetics / Q000378:metabolism; D011925:Rauscher Virus / Q000235:genetics* / Q000472:pathogenicity; D015183:Restriction Mapping; D014162:Transfection
D004279:DNA, Viral