A 62 kDa protein is photoaffinity labelled by [3H]felodipine in vascular smooth muscle, but not in cardiac and skeletal muscle.
Irradiation of a cytosolic fraction from vascular smooth muscle in the presence of [3H]felodipine resulted in the labelling of a protein with an apparent molecular weight of 62 kDa. The labelling was seen on UV-irradiation at 360 nm, but not at 254, 278 or at wavelengths above 410 nm. The photolabelling was enhanced in the absence of oxygen. In cytosolic fractions prepared from porcine liver, cardiac and skeletal muscle no photoaffinity labelling of proteins between 90 and 45 kDa could be demonstrated. The results suggest that felodipine is a photoaffinity ligand and that felodipine binds to a soluble protein present in vascular smooth muscle but not in the other tissues tested.
Nilsson
A
A
Hässle Cardiovascular Research Laboratory, Mölndal, Sweden.
Westerlund
C
C
Boström
S
S
D000345:Affinity Labels; D000818:Animals; D003600:Cytosol / Q000032:analysis; D004095:Dihydropyridines / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D015736:Felodipine / Q000378:metabolism*; D005260:Female; D008297:Male; D009124:Muscle Proteins / Q000302:isolation & purification / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D009132:Muscles / Q000032:analysis*; D009206:Myocardium / Q000032:analysis*; D013552:Swine
D000345:Affinity Labels; D004095:Dihydropyridines; D009124:Muscle Proteins; D015736:Felodipine