title: Isolation and characterization of s-myc, a member of the rat myc gene family.
abstract: A 9-kilobase-pair clone containing a myc-related gene that we have designated s-myc was isolated from a rat genomic library. The entire nucleotide sequence of the cloned DNA was determined, showing that the s-myc gene contains an open reading frame consisting of 1287 base pairs without introns. In vitro transcription-translation analysis of s-myc indicated that this gene produces a protein of approximately 50 kDa. The amino acid sequence predicted from the DNA sequence showed that the s-Myc protein is closely related to the murine N-Myc protein but lacks an acidic amino acid-rich sequence commonly present in the Myc-family proteins. Studies on transfection of s-myc into rat RT4-AC tumor cells revealed that the gene produces a polyadenylylated transcript of approximately 4.7 kilobases and that its high-level expression suppresses the tumor-igenicity of RT4-AC tumor cells in nude mice.
authors.affiliation: Biophysics Division, National Cancer Center Research Institute, Tokyo, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Sugiyama
authors.forename: A
authors.initials: A
authors.lastname: Kume
authors.forename: K
authors.initials: K
authors.lastname: Nemoto
authors.forename: S Y
authors.initials: SY
authors.lastname: Lee
authors.forename: Y
authors.initials: Y
authors.lastname: Asami
authors.forename: F
authors.initials: F
authors.lastname: Nemoto
authors.forename: S
authors.initials: S
authors.lastname: Nishimura
authors.forename: Y
authors.initials: Y
authors.lastname: Kuchino
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods; D004273:DNA, Neoplasm / Q000235:genetics* / Q000302:isolation & purification; D015698:Genomic Library; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D009687:Nuclear Proteins / Q000235:genetics*; D010750:Phosphoproteins / Q000235:genetics*; D010957:Plasmids; D014176:Protein Biosynthesis; D011519:Proto-Oncogenes; D051381:Rats; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D004273:DNA, Neoplasm; D009687:Nuclear Proteins; D010750:Phosphoproteins