title: Secretory protein synthesis in Chironomus salivary gland cells is not coupled with protein translocation across endoplasmic reticulum membranes. Electron microscopic evidence.
abstract: Fragments of rough endoplasmic reticulum containing polysomes bound to the membranes only at the 5 end were visualized in electron microscopic spreads from Chironomus thummi salivary gland cells. The length of the nascent protein molecules in the polysomes increased from the 5 to the 3 (free) polysome end. The data obtained disagree with the generally accepted model according to which synthesis of secretory proteins is concomitant with the protein transport across the endoplasmic membrane.
authors.affiliation: Institute of Cytology and Genetics, Academy of Sciences, USSR Siberian Division, Novosibirsk.
authors.forename: E V
authors.initials: EV
authors.lastname: Kiseleva
mesh_terms: D000818:Animals; D001692:Biological Transport; D002683:Chironomidae; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D007425:Intracellular Membranes / Q000378:metabolism; D007814:Larva; D008854:Microscopy, Electron; D011132:Polyribosomes / Q000378:metabolism*; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D012469:Salivary Glands / Q000378:metabolism / Q000648:ultrastructure; D012471:Salivary Proteins and Peptides / Q000378:metabolism*
chemical_list: D011506:Proteins; D012333:RNA, Messenger; D012471:Salivary Proteins and Peptides