title: Fibronectin gene expression during limb cartilage differentiation.
abstract: A critical event in limb cartilage differentiation is a transient cellular condensation process in which prechondrogenic mesenchymal cells become closely juxtaposed and interact with one another prior to initiating cartilage matrix deposition. Fibronectin (FN) has been suggested to be involved in regulating the onset of condensation and chondrogenesis by actively promoting prechondrogenic aggregate formation during the process. We have performed a systematic quantitative study of the expression of the FN gene during the progression of chondrogenesis in vitro and in vivo. In high-density micromass cultures of limb mesenchymal cells, FN mRNA levels increase about 5-fold coincident with the crucial condensation process, and remain relatively high during the initial deposition of cartilage matrix by the cells. Thereafter, FN mRNA levels progressively decline to relatively low levels as the cultures form a virtually uniform mass of cartilage. The changes in FN mRNA levels in vitro are paralleled closely by changes in the relative rate of FN synthesis as determined by pulse-labeling and immunoprecipitation analysis. The relative rate of FN synthesis increases 4- to 5-fold at condensation and the onset of chondrogenesis, after which it progressively declines to low levels as cartilage matrix accumulates. High levels of FN gene expression also occur at the onset of chondrogenesis in vivo. In the proximal central core regions of the limb bud in which condensation and cartilage matrix deposition are being initiated, FN mRNA levels and the relative rates of FN synthesis become progressively about 4-fold higher than in the distal subridge region, which consists of undifferentiated mesenchymal cells that have not yet initiated condensation.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Anatomy, University of Connecticut Health Center, Farmington 06032.
authors.forename: W M
authors.initials: WM
authors.lastname: Kulyk
authors.forename: W B
authors.initials: WB
authors.lastname: Upholt
authors.forename: R A
authors.initials: RA
authors.lastname: Kosher
mesh_terms: D000818:Animals; D002356:Cartilage / Q000166:cytology / Q000196:embryology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D002642:Chick Embryo; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005353:Fibronectins / Q000096:biosynthesis / Q000235:genetics*; D015870:Gene Expression; D005796:Genes; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D005353:Fibronectins; D012333:RNA, Messenger; D004247:DNA