Age-related reduced affinity in [3H]nitrendipine labeling of brain voltage-dependent calcium channels.
Literature data indicate a reduced calcium uptake in synaptosomes prepared from old rat brains. On this line, the present paper investigates the binding of ([3H]NDP) to brain synaptic membranes prepared from rats at different ages from birth up to 24 months of age. The binding is undetectable at birth but reaches within 9-18 day the values observed in adults [3H]NDP binding affinity and sensitivity to calcium were decreased in old rats (24 months). Tritiated dihydropyridines are believed to label voltage-dependent calcium channels (VDC). The observed age-related reduction in binding suggests that the characteristics of VDC in the aged brain may change.
Govoni
S
S
Rius
R A
RA
Battaini
F
F
Bianchi
A
A
Trabucchi
M
M
D000367:Age Factors; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism*; D005260:Female; D007473:Ion Channels / Q000378:metabolism*; D008297:Male; D009543:Nifedipine / Q000031:analogs & derivatives* / Q000378:metabolism; D009568:Nitrendipine; D051381:Rats; D011919:Rats, Inbred Strains
D007473:Ion Channels; D009568:Nitrendipine; D009543:Nifedipine; D002118:Calcium