Immunological identity of a 60 kd oncofetal protein induced in rats by chemical carcinogens and released by transformed cells.
A 60,000 dalton (60 kd) oncofetal protein was previously shown to be produced by tumors in tumor-bearing rats and by target tissues within 3 weeks of carcinogen treatment. The factor is released to and accumulates in the blood in vivo and in the conditioned medium of cultured transformed cells in vitro. A polyclonal antibody produced against the 60 kd factor purified from the plasma of a rat carrying the N-2-fluorenylphthalamic acid-induced transplantable Hepatoma 7777, was tested against the 60 kd factor from various sources. Based on the results of immunoprecipitation of biochemical activity associated with the 60 kd factor, it was determined that these anti-60 kd antibodies cross-reacted with the factor released by a dimethylbenzanthracene-induced rat mammary carcinoma, with the factor in rat tumor cytosol and with rat spontaneous lymphoma cells, but not with a 60 kd factor isolated from pooled cancer patient plasma. Furthermore, these antibodies cross-reacted with the 60 kd factor induced within 21 days of treatment of the rats with a range of carcinogens from 8 chemical structural groups. The anti-60 kd factor antibodies did not cross-react with a 35 kd factor having similar biochemical activity found in normal adult cells.
Hanausek-Walaszek
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Lang
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Walaszek
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Webb
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D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D000906:Antibodies / Q000276:immunology; D000951:Antigens, Neoplasm / Q000276:immunology; D002273:Carcinogens / Q000494:pharmacology*; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D000939:Epitopes / Q000276:immunology; D005260:Female; D007163:Immunosorbent Techniques; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000378:metabolism; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000378:metabolism; D009368:Neoplasm Transplantation; D051381:Rats; D011915:Rats, Inbred BUF; D011919:Rats, Inbred Strains
D000906:Antibodies; D000951:Antigens, Neoplasm; D002273:Carcinogens; D000939:Epitopes; C031192:oncofetal antigens; D015127:9,10-Dimethyl-1,2-benzanthracene