title: Transcriptional and post-transcriptional regulation of storage protein gene expression in sulfur-deficient pea seeds.
abstract: We have used in vitro transcription in isolated pea nuclei to determine whether the expression of genes for the seed storage proteins legumin and vicilin is regulated transcriptionally or post-transcriptionally in response to changing sulfur status in the developing seed. During the first 48 h of recovery from S deficiency, transcription of legumin genes increased by two-fold whereas the level of legumin mRNA increased 20-fold in the same period. Vicilin gene transcription decreased during recovery, consistent with a gradual decrease in mRNA levels. We conclude that regulation of legumin mRNA levels in response to changing S status is post-transcriptional, whereas that of vicilin mRNA is transcriptional. In contrast, during normal plant development the expression of both of these gene families appears to be under transcriptional control.
authors.forename: L R
authors.initials: LR
authors.lastname: Beach
authors.forename: D
authors.initials: D
authors.lastname: Spencer
authors.forename: P J
authors.initials: PJ
authors.lastname: Randall
authors.forename: T J
authors.initials: TJ
authors.lastname: Higgins
mesh_terms: D005786:Gene Expression Regulation; D009693:Nucleic Acid Hybridization; D063245:Plant Development; D010940:Plant Proteins; D014674:Plant Proteins, Dietary / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D012313:RNA / Q000096:biosynthesis; D055314:Seed Storage Proteins; D012639:Seeds / Q000378:metabolism; D013455:Sulfur / Q000502:physiology*; D014158:Transcription, Genetic; D000097793:Legumins
chemical_list: D010940:Plant Proteins; D014674:Plant Proteins, Dietary; D012313:RNA; D055314:Seed Storage Proteins; D013455:Sulfur; D000097793:Legumins; C015575:vicilin protein, plant