title: A cytocidal tissue kallikrein isolated from mouse submandibular glands.
abstract: A cytocidal factor against mouse thymocytes was purified from the submandibular glands of female BALB/c mice using Sephadex G-50 gel filtration chromatography and reverse-phase HPLC. SDS-PAGE and amino acid sequence analysis revealed that the cytocidal factor was mouse glandular kallikrein (mGK)-6. mGK-6 showed an optimal enzyme activity at pH 10 and a cytocidal activity against thymocytes in a dose-dependent manner.
authors.affiliation: Department of Bacteriology and Immunology, Showa University School of Medicine, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Murakami
authors.forename: H
authors.initials: H
authors.lastname: Ikigai
authors.forename: N
authors.initials: N
authors.lastname: Nagumo
authors.forename: M
authors.initials: M
authors.lastname: Tomita
authors.forename: T
authors.initials: T
authors.lastname: Shimamura
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002470:Cell Survival; D002850:Chromatography, Gel; D003603:Cytotoxins / Q000302:isolation & purification*; D005260:Female; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007610:Kallikreins / Q000302:isolation & purification* / Q000378:metabolism; D008214:Lymphocytes / Q000166:cytology; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D013363:Submandibular Gland / Q000201:enzymology*
chemical_list: D003603:Cytotoxins; D007610:Kallikreins