title: Molecular cloning and characterization of a gene coding for methanol oxidase in Hansenula polymorpha.
abstract: The structural gene and the regulatory DNA sequence of the yeast Hansenula polymorpha methanol oxidase have been isolated. According to the nucleotide sequence data obtained, the structural gene encodes a 664 amino acids long protein, contains no intervening sequences, and the 5'- and 3'-non-coding region contains several sequences implicated in transcription initiation and termination in the yeast Saccharomyces cerevisiae. Although the methanol oxidase is translocated to the peroxisomes, no cleavable signal sequence was found at the N-terminus of the protein.
authors.forename: A M
authors.initials: AM
authors.lastname: Ledeboer
authors.forename: L
authors.initials: L
authors.lastname: Edens
authors.forename: J
authors.initials: J
authors.lastname: Maat
authors.forename: C
authors.initials: C
authors.lastname: Visser
authors.forename: J W
authors.initials: JW
authors.lastname: Bos
authors.forename: C T
authors.initials: CT
authors.lastname: Verrips
authors.forename: Z
authors.initials: Z
authors.lastname: Janowicz
authors.forename: M
authors.initials: M
authors.lastname: Eckart
authors.forename: R
authors.initials: R
authors.lastname: Roggenkamp
authors.forename: C P
authors.initials: CP
authors.lastname: Hollenberg
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics*; D000595:Amino Acid Sequence; D001203:Ascomycota / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004720:Endonucleases / Q000378:metabolism; D009690:Nucleic Acid Conformation; D010843:Pichia / Q000201:enzymology / Q000235:genetics*; D011061:Poly A / Q000032:analysis; D014176:Protein Biosynthesis; D012313:RNA / Q000032:analysis; D012333:RNA, Messenger; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D000429:Alcohol Oxidoreductases; C029293:methanol oxidase; D004720:Endonucleases; D004262:DNA Restriction Enzymes; D015719:Single-Strand Specific DNA and RNA Endonucleases