Novel latex agglutination method with chicken anti-protein A for detection of Staphylococcus aureus infections.
A latex agglutination assay for the detection of protein A-secreting Staphylococcus aureus strains or strains with protein A in the cell wall is described. The assay utilizes latex particles coated with chicken anti-protein A antibodies. Chicken antibodies do not react with protein G-producing streptococci or rheumatoid factor, thus avoiding false-positive reactions.
Larsson
A
A
Department of Medical and Physiological Chemistry, Uppsala University Biomedical Centre, Sweden.
Sjöquist
J
J
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D002645:Chickens; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007841:Latex Fixation Tests; D011237:Predictive Value of Tests; D013203:Staphylococcal Infections / Q000175:diagnosis*; D013205:Staphylococcal Protein A / Q000276:immunology*; D013211:Staphylococcus aureus / Q000276:immunology / Q000302:isolation & purification*
D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A