Reactivation and mutagenesis of herpes virus in 5-azacytidine-treated monkey kidney cells.
Enhanced survival of UV-damaged herpes simplex virus and Simian virus 40 was investigated in CV-1 monkey cells treated with inhibitors of DNA methylation such as 5-azacytidine and ethionine. Survival of UV-irradiated virus was higher in treated cells than in untreated cells. Survival of herpes virus irradiated with 60Co gamma-ray was not enhanced in the treated cells. The frequency of forward mutation of herpes virus increased in 5-azacytidine-treated cells. Relative content of methylcytosine was reduced in the cells treated with 5-azacytidine. Therefore a mechanism similar to UV-enhanced reactivation of virus was operating in the cells with hypomethylated DNA.
Takimoto
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D000818:Animals; D001374:Azacitidine / Q000494:pharmacology*; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004279:DNA, Viral / Q000378:metabolism; D008745:Methylation; D009154:Mutation; D013539:Simian virus 40 / Q000187:drug effects; D018139:Simplexvirus / Q000187:drug effects* / Q000235:genetics / Q000528:radiation effects; D014466:Ultraviolet Rays
D004279:DNA, Viral; D001374:Azacitidine