Immunohistochemical localization of 2',3'-cyclic nucleotide 3'-phosphodiesterase and myelin basic protein in the central nervous system of the jimpy and the normal mouse.
We describe the immunohistochemical localization of 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) and myelin basic protein (MBP) in CNS of the jimpy mutant mouse which is characterized by dys- and demyelination. In controls, the CNPase and MBP were localized exclusively in white matter in the CNS. The jimpy mutant mice were severely affected: A very weak reaction was observed in the white matter. Very few CNPase- and MBP-positive myelin sheaths were observed, and some degradation products were also observed after reaction with antisera against both CNPase and MBP. The immunohistochemical reaction in the jimpy mice showed a similar localization in both CNPase and MBP.
Mikoshiba
K
K
Kohsaka
S
S
Hayakawa
T
T
Takamatsu
K
K
Tsukada
Y
Y
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000032:analysis*; D000818:Animals; D002490:Central Nervous System / Q000032:analysis*; D006651:Histocytochemistry; D051379:Mice; D008816:Mice, Jimpy; D004676:Myelin Basic Protein / Q000032:analysis*; D014018:Tissue Distribution
D004676:Myelin Basic Protein; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases