Cultured human retinal pigment epithelial cells express basic fibroblast growth factor and its receptor.
Basic fibroblast growth factor (bFGF) has been implicated in the maintenance of neuronal differentiation, the induction of neovascularization and intravitreal proliferative diseases. We have found that human retinal pigment epithelial (RPE) cells grown in vitro transcribe the bFGF gene and synthesize a peptide that crossreacts with anti-bFGF antibodies. In culture, these cells appear to release activity with biological and biochemical properties similar to bFGF. RPE cells have specific bFGF receptors and proliferate in response to bFGF. Thus, it is possible that the RPE cell is an important source of retinal bFGF and may respond to bFGF in an autocrine manner.
Sternfeld
M D
MD
Oregon Health Sciences University, Portland 97201.
Robertson
J E
JE
Shipley
G D
GD
Tsai
J
J
Rosenbaum
J T
JT
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002455:Cell Division; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D005346:Fibroblast Growth Factors / Q000096:biosynthesis*; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D010857:Pigment Epithelium of Eye / Q000378:metabolism*; D011061:Poly A / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D011869:Radioligand Assay; D011956:Receptors, Cell Surface / Q000096:biosynthesis*; D017468:Receptors, Fibroblast Growth Factor
D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D017468:Receptors, Fibroblast Growth Factor; D011061:Poly A; D005346:Fibroblast Growth Factors