Transglutaminase-dependent lymphocyte transformation in type 2 diabetes mellitus.
The transglutaminase involvement in phytohaemagglutinin (PHA) stimulated lymphocyte transformation (measured as (3H)thymidine incorporation) was studied in lymphocytes from 20 patients with type 2 diabetes mellitus and 20 healthy controls by including in the system the specific transglutaminase pseudosubstrate monodansylthiacadaverine (MDTC). In the presence of MDTC, (3H)thymidine incorporation was significantly and concentration-dependently reduced in both groups but more pronouncedly in the diabetes patients. The MDTC concentration needed to give a 50% reduction of the PHA-stimulated (3H)thymidine incorporation was significantly lower in lymphocytes from diabetic patients than in those from controls (p less than 0.02). The data suggest impaired lymphocyte transglutaminase function in type 2 diabetes mellitus.
Berntorp
K
K
Department of Medicine, University of Lund, Malmö General Hospital, Sweden.
Eriksson
K F
KF
Berntorp
E
E
D001823:Body Composition; D002103:Cadaverine / Q000031:analogs & derivatives / Q000494:pharmacology; D002784:Cholesterol / Q000097:blood; D003404:Creatinine / Q000097:blood; D004261:DNA Replication / Q000187:drug effects; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000201:enzymology / Q000276:immunology*; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000201:enzymology* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D011503:Transglutaminases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014280:Triglycerides / Q000097:blood
D002103:Cadaverine; D002784:Cholesterol; D003404:Creatinine; D006442:Glycated Hemoglobin; D011503:Transglutaminases; D014280:Triglycerides; C053510:monodansylthiacadaverine