In vivo induction of A/J anti-ARS responses with different ranges of affinities: correlation between affinity and CRIA idiotype dominance.
The anti-ARS immune response of A/J mice is characterized by the reproducible and dominant selection of CRIA bearing antibodies. In this report, we have investigated the role of affinity for the antigen in the selection of antibody repertoire during an immune response. A/J anti-ARS responses with different ranges of affinities for arsonate were elicited by the injection of differently arsanylated carrier proteins. The selection of higher affinity A/J anti-ARS responses was shown to be associated with the induction of higher levels of CRIA bearing anti-ARS antibodies. A detailed idiotopic analysis also showed a more precocious selection of the CRIA "canonical combination" in the higher affinity anti-ARS responses. These results strongly suggest an important role for affinity and clonal selection in the dominant expression of the CRIA idiotype in the A/J anti-ARS response.
Mertens
F
F
Départment de Biologie Moléculaire, Université Libre de Bruxelles, Rhode-St-Genèse, Belgium.
Urbain
J
J
Slaoui
M
M
D000818:Animals; D000915:Antibody Affinity; D000917:Antibody Formation; D001149:Arsenates / Q000276:immunology*; D001151:Arsenic / Q000276:immunology*; D006433:Hemocyanins / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000235:genetics / Q000276:immunology*; D007156:Immunologic Memory; D051379:Mice; D008805:Mice, Inbred A
D001149:Arsenates; D007130:Immunoglobulin Idiotypes; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin; D001151:Arsenic