Prenatal diagnosis of cystic fibrosis. Analytical evaluation of microvillar enzyme determinations in amniotic fluid.
The activity of gamma-glutamyltranspeptidase and total alkaline phosphatase and its isoenzymes has been determined in 261 amniotic fluid samples taken from pregnant women with known normal outcome and in 30 amniotic fluid samples from pregnant women with a 1:4 risk for cystic fibrosis (CF). Preliminarily, 114 amniotic fluid samples were assayed in parallel in three different laboratories, and a good correlation was found even though different assays were used. From the results obtained in control amniotic fluids, normal range and CF-predictive cutoff values were established. No false-negative results were found in this study. Among the predicted affected pregnancies 7 were terminated, and 3 went to term: 1 resulting in a CF-affected child and the other 2 in healthy children. CF was confirmed in all the aborted fetuses. In 1 case the results were inconclusive. In this study numerical results obtained for samples with a 1:4 risk of CF analyzed in the three laboratories were always virtually identical.
Dechecchi
M C
MC
Centro Fibrosi Cistica, Ospedale Civile Maggiore, Verona, Italia.
Girella
E
E
Borgo
G
G
Mastella
G
G
D000469:Alkaline Phosphatase / Q000378:metabolism*; D000653:Amniotic Fluid / Q000201:enzymology*; D015415:Biomarkers / Q000032:analysis*; D003550:Cystic Fibrosis / Q000175:diagnosis*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D007700:Kinetics; D008871:Microvilli / Q000201:enzymology*; D011247:Pregnancy; D011296:Prenatal Diagnosis; D012016:Reference Values; D005723:gamma-Glutamyltransferase / Q000378:metabolism*
D015415:Biomarkers; D007527:Isoenzymes; D005723:gamma-Glutamyltransferase; D000469:Alkaline Phosphatase