Purification and characterization of bacteriophage receptor on Veillonella rodentium cells. Phage-receptor on Veillonella.
Veillonellophage N2 adsorbed to polysaccharides (PSs) on Veillonella rodentium ATCC 17743 cell wall, and the bacteriophage receptor contained only glucosamine. D(+)-glucosamine hydrochloride (Sigma) also adsorbed the veillonellophage N2. These results therefore indicate that the receptor to the veillonellophage N2 is cell wall PSs. The PSs of the host cells as receptor have been characterized. Glucosamine accounted for approximately 100% of the weight of the PSs. The PSs which were partially resolved by Sephadex G-75 chromatography comprised approximately four glucosamine units. Their primary structure was determined by 400 MHz n.m.r. spectroscopy. One- and two-dimensional 1H-nmr experiments showed the PS to be a branched polymer. Glucosamine linkage was detected in one of the branches.
Totsuka
M
M
Department of Oral Bacteriology, Nagasaki University School of Dentistry, Japan.
Ono
T
T
D001435:Bacteriophages / Q000187:drug effects / Q000302:isolation & purification*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D011991:Receptors, Virus / Q000187:drug effects / Q000302:isolation & purification*; D014678:Veillonella / Q000032:analysis*
D008070:Lipopolysaccharides; D011991:Receptors, Virus