Isolation and sequencing of a complementary deoxyribonucleic acid clone encoding human placental 17 beta-estradiol dehydrogenase: identification of the putative cofactor binding site.
17 beta-Estradiol dehydrogenase (EC 1.1.1.62) catalyzes the interconversion of estradiol and estrone in human term placenta. We have raised a specific polyclonal antibody to this abundant placental enzyme to study its role in late pregnancy events and its molecular biologic characteristics. In this work the 17 beta-estradiol dehydrogenase antibody was used to isolate and sequence a complementary deoxyribonucleic acid clone encoding about 98% of the amino acid sequence of the 17 beta-estradiol dehydrogenase molecule. This sequence verifies previous sequence data on the molecule's steroid binding site and also localizes a putative nicotinamide adenine dinucleotide binding region similar to that of many other pyridine nucleotide-dependent dehydrogenases. Isolation of the complementary deoxyribonucleic acid for 17 beta-estradiol dehydrogenase expands our knowledge of the structure-function relationships of the enzyme and is a major step in our understanding of its biologic function in pregnancy.
Gast
M J
MJ
Department of Obstetrics and Gynecology, Washington University School of Medicine, St. Louis, MO 63110.
Sims
H F
HF
Murdock
G L
GL
Gast
P M
PM
Strauss
A W
AW
D015067:17-Hydroxysteroid Dehydrogenases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004960:Estradiol Dehydrogenases / Q000235:genetics*; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D009243:NAD / Q000032:analysis; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy
D009243:NAD; D004247:DNA; D015067:17-Hydroxysteroid Dehydrogenases; D004960:Estradiol Dehydrogenases