Demonstration of a basic fibroblast growth factor-like molecule in mouse hepatic endothelial cells.
We have investigated whether isolated mouse hepatic sinusoidal endothelial cells (HSEC) synthesized basic FGF. HSEC lysate was fractionated by heparin-Sepharose chromatography. A peak of mitogenic activity for Balb/c 3T3 fibroblasts was eluted with 3M NaCl. Several arguments suggested that the mitogenic factor was related to bFGF: a) its affinity for heparin; b) the loss of its mitogenic activity by heating at 65 degrees C, which was prevented in the presence of heparin; c) the abolition of its mitogenic activity in the presence of protamine sulfate; d) finally, its mitogenic effect was reduced in the presence of antibody to bFGF. These data demonstrate the presence of a bFGF-like molecule in HSEC. This molecule could be involved in the regulation of the neighboring Ito cell proliferation.
Rosenbaum
J
J
INSERM U-99, Hôpital Henri Mondor, Creteil, France.
Mavier
P
P
Preaux
A M
AM
Dhumeaux
D
D
D000818:Animals; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects; D004727:Endothelium / Q000032:analysis; D005346:Fibroblast Growth Factors / Q000032:analysis* / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology; D006358:Hot Temperature; D008099:Liver / Q000032:analysis*; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D011489:Protein Denaturation; D011994:Recombinant Proteins / Q000494:pharmacology
D011994:Recombinant Proteins; D005346:Fibroblast Growth Factors; D006493:Heparin