Structure of the sea urchin U1 RNA repeat.
The genes coding for U1 RNA in the sea urchin L. variegatus are present in a 1400 base pair tandem repeat. One member of the repeat has been cloned and its sequence determined. The repeat unit contains a single copy of the gene for L. variegatus U1 RNA. This gene encodes an RNA which is 75% homologous to mammalian U1 RNA. The L. variegatus U1 RNA could assume a secondary structure similar to that proposed for other U1 RNAs. In addition the L. variegatus U1 RNA is precipitated by anti-SM and anti-RNP antisera. Analysis of the L. variegatus genomic DNA using the cloned U1 gene as a probe reveals a major and a minor type of repeat unit. The two repeated units are the same length but differ in a number of restriction enzyme sites clustered 200-500 bases down-stream from the gene. The monomer we have cloned and sequenced is a representative of the minor repeat. A sequence (GATAA) which is -41 to -37 bases 5' to the gene has homology to the putative RNA polymerase II promoter. Fifteen bases 3' of the gene is a sequence (CAAAGAAAGAAAA) which is very similar to the sequence found 3' of the sea urchin histone genes. The two Hha I, Hpa II and Ava I sites in the repeat are all unmethylated in sperm DNA.
Brown
D T
DT
Morris
G F
GF
Chodchoy
N
N
Sprecher
C
C
Marzluff
W F
WF
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D005786:Gene Expression Regulation; D005796:Genes; D009690:Nucleic Acid Conformation; D011110:Polymorphism, Genetic; D012313:RNA / Q000235:genetics*; D012342:RNA, Small Nuclear; D012091:Repetitive Sequences, Nucleic Acid; D012261:Ribonucleoproteins / Q000032:analysis; D012617:Sea Urchins / Q000235:genetics* / Q000254:growth & development; D013045:Species Specificity
D012342:RNA, Small Nuclear; D012261:Ribonucleoproteins; D012313:RNA; D004262:DNA Restriction Enzymes