Monoclonal cell surface antibodies do not produce short-term effects on electrical properties of mouse oligodendrocytes in culture.
Eleven monoclonal antibodies (O1-O11) directed against the surface of oligodendrocytes were applied individually or in combination during measurement of membrane potential, input resistance and K+-pump activity in explant cultures of mouse spinal cord. Antibody binding to oligodendrocytes was verified by indirect immunofluorescence. None of the antibodies affected the electrical properties studied. On the basis of these observations, it is possible to identify oligodendrocytes immunocytologically prior to electrophysiological characterization.
Kettenmann
H
H
Sommer
I
I
Schachner
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000276:immunology*; D002462:Cell Membrane / Q000502:physiology*; D002463:Cell Membrane Permeability; D046508:Culture Techniques; D007473:Ion Channels / Q000502:physiology; D008564:Membrane Potentials; D051379:Mice; D009457:Neuroglia / Q000276:immunology*; D009836:Oligodendroglia / Q000276:immunology*; D011188:Potassium / Q000378:metabolism; D013116:Spinal Cord
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D007473:Ion Channels; D011188:Potassium