title: Inactivation of aztreonam by faecal supernatants of healthy volunteers as determined by HPLC.
abstract: The enzymatic inactivation of aztreonam to its biologically inactive open ring form, by faecal enzyme preparations from 12 volunteers, was investigated. The percentage inactivation was determined by a high-performance liquid chromatography assay at months 0, 6 and 24. Faecal enzyme preparations from five volunteers could inactivate aztreonam by more than 50% within 20 h at one or more of these sampling times. Monitoring of aztreonam inactivation over a shorter period of time (14 days) in three of the volunteers showed that aztreonam inactivation cannot be predicted.
authors.affiliation: Laboratorium voor Medische Microbiologie, Rijksuniversiteit Groningen, The Netherlands.
authors.forename: G W
authors.initials: GW
authors.lastname: Welling
authors.forename: G
authors.initials: G
authors.lastname: Groen
mesh_terms: D000328:Adult; D001398:Aztreonam / Q000037:antagonists & inhibitors*; D002851:Chromatography, High Pressure Liquid; D005243:Feces / Q000201:enzymology*; D005260:Female; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D013056:Spectrophotometry, Ultraviolet; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D007202:Indicators and Reagents; D001618:beta-Lactamases; D001398:Aztreonam