[Alternative methods: development and use of two "in vitro" models for endocrine studies].
Most endocrinological studies are normally performed on several animal groups: one group for the determination of hormonal levels in tissues and plasma under basal conditions, a second group for the same determinations after "in vivo" treatment of the animals (stimulation or inhibition of the endocrine activities) and two additional groups for morphological investigations. Surgical approaches belong not rarely to the "in vivo" treatment of the animals. Moreover, the results from the different animal groups have to be extrapolated. In order to overcome these drawbacks, we have developed two "in vitro" models by means of which we can study on the tissues from the same animal: 1. function and structure of the hypothalamus and 2. function and structure of isolated pituitary cells. By using these models we can considerably reduce the number of animals needed for the studies, replace the "in vivo" by the "in vitro" experiments and refine the methods avoiding, among others, the extrapolation of results.
Bestetti
G E
GE
Boujon
C E
CE
Tontis
D K
DK
Forster
U
U
Grimm
S
S
Rossi
G L
GL
D000818:Animals; D002478:Cells, Cultured; D046508:Culture Techniques; D004704:Endocrinology / Q000379:methods*; D007031:Hypothalamus / Q000033:anatomy & histology / Q000502:physiology*; D008297:Male; D008954:Models, Biological; D010902:Pituitary Gland / Q000166:cytology* / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains
Méthodes alternatives: développement et utilisation de deux modèles'in vitro' pour des études endocriniennes.