title: [Obtaining unidirectional deletions in a plasmid using a DNA-"protector" and Bal31 exonuclease].
abstract: A method of generating unidirectional deletions from the cleavage site of any endonuclease by means of exonuclease Bal31 is developed. DNA sequence to be prevented from Bal31 hydrolysis is protected by a special "protector" element TH (Trojan Horse) inserted upstream of the sequence.
authors.forename: D I
authors.initials: DI
authors.lastname: Nurminskiĭ
authors.forename: Iu Ia
authors.initials: IuIa
authors.lastname: Shevelev
authors.forename: A I
authors.initials: AI
authors.lastname: Kalmykova
mesh_terms: D002872:Chromosome Deletion; D004247:DNA / Q000235:genetics*; D004706:Endodeoxyribonucleases; D010957:Plasmids
chemical_list: D004247:DNA; D004706:Endodeoxyribonucleases; C038407:exonuclease Bal 31
vernacular_title: Poluchenie odnonapravlennykh deletsiĭ deletsiĭ v plazmide s pomoshch'iu DNK-"protektora" i ékzonukleazy Bal31.