title: Intersubunit RNA-protein contacts in pre- and post-translocated E. coli ribosome.
abstract: Ribosomal proteins participating in intersubunit RNA-protein contacts (directly interacting with RNA of the opposite subunit) were determined by means of ultraviolet-induced cross-links in pre- and post-translocated ribosomal complexes, as well as in the free 70 S ribosome (tight couple) of E. coli. In these 3 complexes at least L1 and L9 proteins interact with 16 S RNA, while S6, S9/11 and S15 react with 23 S RNA. All these proteins ('hinge-joint' proteins) are clustered on the small protuberance of the 50 S subunit and on the platform of the 30 S subunit. Reduction in the number of other (variable) intersubunit RNA-protein contacts in the course of transition from the tight couple to the pre- and, finally, to the post-translocated state, demonstrates gradual loosening of intersubunit interactions in 70 S ribosome. Such a loosening ('opening') of the 70 S ribosome is determined by conformational changes in ribosomal subunits and/or in their relative arrangement, conjugated with alteration of the functional state of the ribosomal complex.
authors.forename: G G
authors.initials: GG
authors.lastname: Abdurashidova
authors.forename: E A
authors.initials: EA
authors.lastname: Tsvetkova
authors.forename: A A
authors.initials: AA
authors.lastname: Chernyi
authors.forename: L B
authors.initials: LB
authors.lastname: Kaminir
authors.forename: E I
authors.initials: EI
authors.lastname: Budowsky
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism / Q000528:radiation effects; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation / Q000528:radiation effects; D012329:RNA, Bacterial / Q000378:metabolism; D012335:RNA, Ribosomal / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000378:metabolism* / Q000528:radiation effects; D014178:Translocation, Genetic; D014466:Ultraviolet Rays
chemical_list: D001426:Bacterial Proteins; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins