Structural and immunological properties of myosin from human platelet external and internal membranes.
Our previous studies indicate that platelets contain two myosin isoforms, one of them localized in the membrane while the other in the cytoplasmic compartment. Structural and functional differences of these myosins have been characterized. In this study two platelet membrane subfractions, the external and the internal membranes, were isolated simultaneously from a crude membrane fraction and their purity was characterized using specific marker enzymes. Myosin was shown to be present in both membrane fractions and its structural and immunological properties were investigated. The electrophoretic mobilities of myosin in both membrane preparations were identical to the mobility of its cytoplasmic counterpart. Two-dimensional peptide mapping of the iodinated tryptic peptides of the myosin heavy chains indicated that at least one peptide is missing in the maps of the myosins from the external and internal membranes as compared to their soluble counterpart. Our data suggest that myosin is located in three distinct platelet compartments: cytosol, external and internal membranes. The same myosin isoform is located in the two membrane compartments, while the isoform found in the cytosol is different. The observed variations in the structure of the two isoforms may reflect differences in their respective physiological functions.
Peleg
I
I
Department of Membrane and Ultrastructure Research, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Kahane
I
I
Eldor
A
A
Groschel-Stewart
U
U
Levy-Toledano
S
S
Muhlrad
A
A
D001792:Blood Platelets / Q000032:analysis*; D002462:Cell Membrane / Q000032:analysis; D006801:Humans; D009218:Myosins / Q000032:analysis* / Q000276:immunology; D010449:Peptide Mapping
D009218:Myosins