Multiple labelling of immunoglobulin G, albumin and testosterone with a fluorescent terbium chelate for fluorescence immunoassays.
Human immunoglobulin G, human serum albumin and testosterone were labelled with the 4-aminosalicylic acid derivative of diethylenetriaminepentaacetic acid complexed with terbium ions. An exceptionally large amount of label, of the order of a few hundred moles of complex per mole of analyte, could be conjugated to the compounds tested by the use of poly-L-lysine. Self-quenching appears to be minimal, even with this high local concentration of fluorophores. The tracers were stable at 4 degrees C, and gave competitive calibration graphs at physiological concentrations.
Canfi
A
A
Bailey
M P
MP
Rocks
B F
BF
D002614:Chelating Agents; D005453:Fluorescence; D006801:Humans; D007118:Immunoassay; D007074:Immunoglobulin G / Q000032:analysis*; D004369:Pentetic Acid; D012709:Serum Albumin / Q000032:analysis*; D013725:Terbium / Q000378:metabolism*; D013739:Testosterone / Q000032:analysis*
D002614:Chelating Agents; D007074:Immunoglobulin G; D012709:Serum Albumin; D013725:Terbium; D013739:Testosterone; D004369:Pentetic Acid