Tyrosine hydroxylase immunohistochemistry in the normal and 1-methyl-4-phenyl-tetrahydropyridine (MPP+)-treated retina of goldfish.
Dopaminergic interplexiform neurons have been identified in the inner nuclear layer of goldfish retina, with tyrosine hydroxylase (TH) immunocytochemistry in whole-mounted retinae and in cryosections. The neurotoxin 1-methyl-4-phenylpyridinium ion (MPP+), which selectively damages dopaminergic neurons in mammals, caused a marked depletion of TH immunoreactivity in goldfish retina. Three days after intravitreal injection, retinae showed no significant decrease in the number of TH-positive neurons. However most of the TH-immunoreactive cell bodies showed an evident depletion of TH immunoreactivity and their processes, ramified in the inner and outer plexiform layers, disappeared almost completely.
Guarnieri
T
T
Department of Biology, University of Bologna, Italy.
Villani
L
L
Fasolo
A
A
D015655:1-Methyl-4-phenylpyridinium / Q000494:pharmacology*; D000818:Animals; D003530:Cyprinidae / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D006054:Goldfish / Q000033:anatomy & histology / Q000378:metabolism*; D007150:Immunohistochemistry; D012160:Retina / Q000187:drug effects / Q000201:enzymology*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*
D014446:Tyrosine 3-Monooxygenase; D015655:1-Methyl-4-phenylpyridinium; D004298:Dopamine