title: High-performance liquid chromatographic method for the determination of 9-(4-hydroxy-3-hydroxymethylbut-1-yl)guanine (BRL-39123) in human plasma and urine.
abstract: A rapid, sensitive and reliable reversed-phase high-performance liquid chromatographic (HPLC) method with UV detection has been developed for the assay of a novel anti-herpes agent, 9-(4-hydroxy-3-hydroxymethylbut-1-yl)guanine (BRL-39123), in human plasma and urine. The drug and the internal standard, the structural analogue BRL-42377, were extracted from the biological matrix by adsorption on a cation-exchange column and were subsequently eluted under alkaline conditions prior to HPLC. The method is reproducible, with coefficients of variation of ca. 5%, and linear from 0.1 to at least 30 micrograms ml-1 in plasma and from 50 to at least 2000 micrograms ml-1 in urine. The method has been used extensively to measure BRL-39123 in plasma and urine samples generated during clinical studies and is adequate for defining pharmacokinetics at projected therapeutic doses.
authors.forename: S E
authors.initials: SE
authors.lastname: Fowles
authors.forename: D M
authors.initials: DM
authors.lastname: Pierce
mesh_terms: D000212:Acyclovir / Q000031:analogs & derivatives* / Q000032:analysis; D000998:Antiviral Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D006147:Guanine; D006801:Humans
chemical_list: D000998:Antiviral Agents; C053539:penciclovir; D006147:Guanine; D000212:Acyclovir