[Z-line structure of the rabbit psoas muscle studied by negative contrast staining].
The ultrastructure of the Z-disc of the rabbit psoas muscle was elucidated by electron microscopy using negative staining technique. Conclusions summarized from this work are as follow: (a) Z-disc involves two layers of Z-filaments, i.e. connecting filaments, which bind thin filaments of adjacent I-discs in the Z-line region. These layers are spaced about 380 A apart. (b) Z-filaments measure 380 A X 30 A. (c) The angle between the connecting filaments and the thin filaments depends on ionic conditions and varies from 20 degrees to 90 degrees. (d) We conclude that alpha-actinin is a structural component of Z-filaments, since dimensions of Z-filaments and their interaction with thin filaments are similar to those of alpha-actinin.
Tskhovrebova
L A
LA
Podlubnaia
Z A
ZA
D000185:Actinin / Q000032:analysis; D000818:Animals; D008854:Microscopy, Electron; D009210:Myofibrils / Q000648:ultrastructure*; D011817:Rabbits; D013194:Staining and Labeling / Q000379:methods
D000185:Actinin
Issledovanie struktury Z-linii poiasnichnoĭ myshtsy krolika metodom negativnogo kontrastirovaniia.