Regulation of the gene for estrogenic 17-ketosteroid reductase lying on chromosome 17cen----q25.
17-Ketosteroid reductase (17KSR), also known as 17 beta-hydroxysteroid dehydrogenase, catalyzes the reversible interconversion of estradiol to estrone and of androstenedione to testosterone. Using a recently cloned human placental 17KSR cDNA, we show that the 1.4-kilobase mRNA for this enzyme is detected only in tissues producing estrogens, and a 2.4-kilobase mRNA is detected in some estrogenic tissues and some androgenic tissues. This tissue distribution suggests that the interconversion of androstenedione and testosterone may be mediated by a different enzyme. Southern blotting studies show that the mRNA for this estrogenic 17KSR is encoded by two very similar genes localized to chromosome 17cen----q25 by analysis of DNA from mouse/human somatic hybrid cell lines. 8-Br-cAMP increases the abundance of estrogenic 17KSR mRNA as well as mRNAs for other steroidogenic enzymes in JEG-3 choriocarcinoma cells. By contrast, cAMP decreases estrogenic 17KSR mRNA in primary cultures of human cytotrophoblasts and human granulosa cells, a pattern of tropic regulation that differs from other steroidogenic enzyme mRNAs.
Tremblay
Y
Y
Department of Pediatrics, University of California, San Francisco 94143.
Ringler
G E
GE
Morel
Y
Y
Mohandas
T K
TK
Labrie
F
F
Strauss
J F
JF
Miller
W L
WL
D015067:17-Hydroxysteroid Dehydrogenases / Q000235:genetics*; D000818:Animals; D015139:Blotting, Southern; D002460:Cell Line; D002874:Chromosome Mapping; D002886:Chromosomes, Human, Pair 17; D004247:DNA / Q000235:genetics; D004960:Estradiol Dehydrogenases / Q000235:genetics*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D005796:Genes; D006107:Granulosa Cells / Q000201:enzymology; D006801:Humans; D006822:Hybrid Cells / Q000201:enzymology; D008214:Lymphocytes / Q000201:enzymology; D051379:Mice; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D015183:Restriction Mapping; D014158:Transcription, Genetic
D012333:RNA, Messenger; D004247:DNA; D015067:17-Hydroxysteroid Dehydrogenases; D004960:Estradiol Dehydrogenases