Flow cytometric analysis of murine splenic cells after DNFB painting on Langerhans cell deficient skin.
One week after a single painting of 50 microliters of 0.5% DNFB in acetone-olive oil on the tail skin of C3H mice, the spleens were removed from the animals. A single cell suspension was prepared from the spleens, and flow cytometric analysis was performed for L3T4 and Lyt2 positive cells, as well as for the IJk expression of the Lyt2+ cells. Results showed that the percentages and absolute numbers of Lyt2+ L3T4- cells, Lyt2- L3T4+ cells, Lyt2+ IJk+ cells, and Lyt2+ IJk- cells in the spleens of the DNFB-treated mice were not significantly different from those in the non-treated mice. However, in the treated mice, the IJk expression of Lyt2+ cells intensified. These results indicate that, following a single painting of DNFB onto Langerhans cell-deficient skin, the numbers of Lyt2+ cells do not change significantly, but do change functionally.
Morita
H
H
Kihara
T
T
Kuramoto
M
M
Minami
S
S
Yu
M
M
Yamagata
M
M
Sagami
S
S
D000818:Animals; D004139:Dinitrofluorobenzene / Q000494:pharmacology*; D005260:Female; D005434:Flow Cytometry; D007801:Langerhans Cells; D051379:Mice; D008809:Mice, Inbred C3H; D009578:Nitrobenzenes / Q000494:pharmacology*; D012867:Skin / Q000166:cytology / Q000187:drug effects*; D013154:Spleen / Q000166:cytology*
D009578:Nitrobenzenes; D004139:Dinitrofluorobenzene