Substitution of asparagine324 with aspartic acid in the Fc portion of mouse antibodies reduces their capacity for C1q binding.
The sequence of the heavy chain C region of mouse mutant IgG2a antibodies with reduced capacity for C1q binding but with retained ability for Fc receptor-mediated functions was determined by cDNA cloning and by mRNA sequencing. The specific mutation was found to be the substitution of asparagine324 with aspartic acid. Asparagine324 represents a new residue relating to the C1q-binding sites previously described.
Nose
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Department of Pathology, Tohoku University School of Medicine, Sendai, Japan.
Leanderson
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D000595:Amino Acid Sequence; D000818:Animals; D001216:Asparagine; D001224:Aspartic Acid; D001483:Base Sequence; D015922:Complement C1q / Q000378:metabolism*; D007141:Immunoglobulin Fc Fragments / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D013329:Structure-Activity Relationship
D007141:Immunoglobulin Fc Fragments; D001224:Aspartic Acid; D001216:Asparagine; D015922:Complement C1q