Self reactive delayed type hypersensitivity (DTH) induced in mice by syngeneic lymphoblasts.
Normal and x-irradiated A mice injected with syngeneic concanavalin A (Con A)-induced lymphoblasts revealed after challenge with syngeneic lipopolysaccharide (LPS)-induced lymphoblasts delayed type hypersensitivity (DTH) measured both by footpad swelling and by 125IudR accumulation. Mice injected with allogeneic Con A-induced lymphoblasts and challenged with syngeneic LPS-induced lymphoblasts or vice versa, also generated an appreciable DTH response. In contrast, Con A-induced blast cells of human origin (xenogeneic cells) generated a considerably less effective DTH. The DTH response was more profound and consistent in x-irradiated mice, suggesting that irradiation sensitive cells control this response. The syngeneic DTH response was efficiently transferred to naive recipients with Thy-1+, nylon wool passed cells. The establishment of the DTH activity was associated with the lymphoblasts own (differentiation?) antigens and not with contaminants attached to the cells, such as Con A or fetal calf serum. The results were compared with similar results reported by other groups and the biological significance of all findings was evaluated.
Klein
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Naor
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D000818:Animals; D001324:Autoantigens / Q000276:immunology; D001327:Autoimmune Diseases / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007111:Immunity, Cellular; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008805:Mice, Inbred A / Q000276:immunology*; D008815:Mice, Inbred Strains / Q000276:immunology
D001324:Autoantigens; D000939:Epitopes