High-performance liquid chromatographic analysis of the enantiomers of flurbiprofen and its metabolites in plasma and urine.
Reversed-phase high-performance liquid chromatographic methods have been developed to quantitate the R- and S-enantiomers of flurbiprofen and its major metabolites, 4'-hydroxyflurbiprofen, 3'-hydroxy-4'-methoxyflurbiprofen, and 3',4'-dihydroxyflurbiprofen. The compounds are extracted from plasma or urine and derivatized with S-(alpha)-methylbenzylamine to form diastereomeric amides which are readily separated on a C18 column. Fluorescence detection resulted in detection limits that readily allowed us to characterize the disposition of R- and S-flurbiprofen and its major metabolites in man following therapeutic doses.
Knadler
M P
MP
Indiana University Medical School, Wishard Memorial Hospital, Indianapolis 46202.
Hall
S D
SD
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005480:Flurbiprofen / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D010627:Phenethylamines; D013237:Stereoisomerism
D000894:Anti-Inflammatory Agents, Non-Steroidal; D010627:Phenethylamines; D005480:Flurbiprofen; C033198:1-phenethylamine