Occurrence of the methylisobutylxanthine-stimulated cyclic GMP binding protein in various rat tissues.
A new type of cGMP binding protein, the activity of which is characteristically stimulated by methylisobutylxanthine, has been previously discovered in rat lung and platelets (Hamet, P. and Coquil, J.F. (1978) J. Cyclic Nucleotide Res. 4, 281-290). In the present study, we demonstrate the occurrence of this protein in soluble extracts of a variety of rat tissues fractionated by a DEAE-Sepharose chromatography. In several tissues (spleen, lung and brain) the binding activity of this protein was of the same order of magnitude as that of the cGMP-dependent protein kinase.
Coquil
J F
JF
Brunelle
G
G
Guédon
J
J
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology*; D000818:Animals; D001792:Blood Platelets / Q000032:analysis; D001923:Brain Chemistry; D002352:Carrier Proteins / Q000378:metabolism*; D002852:Chromatography, Ion Exchange; D047908:Intracellular Signaling Peptides and Proteins; D008168:Lung / Q000032:analysis; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000032:analysis; D013806:Theophylline / Q000031:analogs & derivatives*; D014018:Tissue Distribution
D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; C022994:cyclic GMP-binding protein; D013806:Theophylline; D015056:1-Methyl-3-isobutylxanthine