[Sex determination of the embryo by DNA studies of chorionic villi samples].
The first step in the prenatal diagnosis of X-linked genetic disorders is the determination of the sex of the fetus. A new method for this purpose is based on recombinant DNA technology. The authors give a short account on their experiences with a Y specific DNA probe. Fetal DNA was prepared from chorionic villi taken at the 8th-12th weeks of gestation. The DNA was hybridised with the Y specific probe. This probe was isolated from the 3,4 kilobase human repeat sequence derived from heterochromatin of the Y chromosome and had 1000 times more affinity for male DNA than for female DNA. The method based on hybridisation with the Y specific probe should facilitate first-trimester prenatal sex determination of X-linked genetic disorders.
Németi
M
M
Bolodár
A
A
Horváth
K
K
Papp
Z
Z
D000029:Abortion, Legal; D000328:Adult; D015193:Chorionic Villi Sampling; D004247:DNA / Q000032:analysis; D005260:Female; D005817:Genetic Counseling; D006467:Hemophilia A / Q000235:genetics; D006801:Humans; D006814:Hungary; D007621:Karyotyping; D009136:Muscular Dystrophies / Q000235:genetics; D011247:Pregnancy; D011296:Prenatal Diagnosis; D012729:Sex Chromosome Aberrations / Q000175:diagnosis*; D012732:Sex Determination Analysis
D004247:DNA
Az embryo nemének meghatározása chorionboholy-mintából DNS vizsgálattal.