Monoclonal antibodies localize the exchangeable GTP-binding site in beta- and not alpha-tubulins.
A combination of several methods was used to localize the exchangeable GTP-binding site in the alpha/beta-tubulin heterodimer: direct photoaffinity labeling with [alpha-32P]GTP, specific labeling of alpha- and beta-tubulin by tyrosylation and phosphorylation, respectively, and immunoprecipitation with specific monoclonal antibodies. Direct evidence was obtained that GTP binds exclusively to beta- and not alpha-tubulins.
Hesse
J
J
Maruta
H
H
Isenberg
G
G
D000818:Animals; D000911:Antibodies, Monoclonal; D001665:Binding Sites; D001921:Brain / Q000378:metabolism; D002417:Cattle; D000939:Epitopes / Q000032:analysis; D006160:Guanosine Triphosphate / Q000378:metabolism*; D007700:Kinetics; D046911:Macromolecular Substances; D011485:Protein Binding; D014404:Tubulin / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D046911:Macromolecular Substances; D014404:Tubulin; D006160:Guanosine Triphosphate