Influence of monosaccharides on the fibrillogenesis of type I collagen.
The in vitro self-assembly of Type I rat tail tendon collagen in the presence of monosaccharides was studied turbidimetrically. D(+)glucose and D(+)mannose delayed fibril formation significantly while D(-)fructose showed no variation. The effect of glucose was concentration dependent. Binding of glucose during fibrillogenesis was shown by the incorporation of [14C]glucose and also by SDS-polyacrylamide slab gel electrophoresis of the product. The results indicate that in vitro nonenzymatic glycosylation can take place in the short duration required for fibril formation.
Rathi
A N
AN
Biological Sciences Laboratory, Central Leather Research Institute, Madras, India.
Padmavathi
S
S
Chandrakasan
G
G
D000818:Animals; D003094:Collagen / Q000378:metabolism* / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D006207:Half-Life; D007700:Kinetics; D009005:Monosaccharides / Q000494:pharmacology*; D009391:Nephelometry and Turbidimetry; D051381:Rats
D009005:Monosaccharides; D003094:Collagen