Isolation and initial characterization of a temperature-sensitive mutant of mouse FM3A cells defective in cytokinesis.
A temperature-sensitive mutant, designated tsFT101, was isolated from a mouse mammary carcinoma cell line, FM3A, and given an initial characterization. In this cell line, cytokinesis was blocked at a non-permissive temperature (39 degrees C), but DNA synthesis and nuclear division proceeded normally for at least 24 h at 39 degrees C as detected respectively by autoradiography and cytofluorometric analysis. As a result, multinucleate cells accumulated at 39 degrees C (more than 95% in 36 h). When the culture was returned to a permissive temperature (33 degrees C) after 24 h of arrest at 39 degrees C, cytokinesis was resumed and there was a rapid decrease in the number of multinucleate cells. At 39 degrees C, tsFT101 cells had less F-actin than cells at 33 degrees C, indicative of the existence of an abnormality in actin polymerization in this mutant.
Yasumitsu
H
H
Hanaoka
F
F
Yasuda
H
H
Murakami
Y
Y
Enomoto
T
T
Yamada
M
M
D000199:Actins / Q000502:physiology; D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002467:Cell Nucleus / Q000502:physiology; D003599:Cytoskeleton / Q000502:physiology; D005260:Female; D008325:Mammary Neoplasms, Experimental / Q000473:pathology; D051379:Mice; D008938:Mitosis; D009154:Mutation; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D013696:Temperature
D000199:Actins; D012313:RNA