title: Degradation of linear DNA by a strand-specific exonuclease activity in Xenopus laevis oocytes.
abstract: Linear DNA injected into Xenopus laevis oocyte nuclei recombines with high efficiency if homologous sequences are present at overlapping molecular ends. We found that injected linear DNA was degraded by a 5'----3' strand-specific exonuclease activity during incubation in the oocyte nucleus to leave a heterogeneous population of 3'-tailed molecules. Decreasing the concentration of DNA injected increased the heterogeneity and the average rate of degradation. The 3' tails created were relatively stable; among molecules persisting after overnight incubation, many had 3' tails intact to within 10 bases of the original ends. DNA molecules that were efficient substrates for homologous recombination in oocytes were also partially degraded, leaving 3' tails. We found no evidence for other potent nuclease activities. If molecules with recessed 3'-OH ends were injected, endogenous polymerase efficiently resynthesized complementary strands before degradation of the 5' tails occurred. 3'-tailed molecules are plausible intermediates in the initiation of homologous recombination events in Xenopus oocyte nuclei.
authors.affiliation: Department of Cellular, Viral and Molecular Biology, University of Utah School of Medicine, Salt Lake City 84132.
authors.forename: E
authors.initials: E
authors.lastname: Maryon
authors.forename: D
authors.initials: D
authors.lastname: Carroll
mesh_terms: D000818:Animals; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D005090:Exodeoxyribonucleases / Q000378:metabolism*; D005260:Female; D009693:Nucleic Acid Hybridization; D009865:Oocytes / Q000201:enzymology / Q000378:metabolism*; D010957:Plasmids; D011995:Recombination, Genetic; D014982:Xenopus laevis
chemical_list: D004247:DNA; D005090:Exodeoxyribonucleases