[Neural differentiation of ectodermal explants of the early gastrula from Rana temporaria under the action of various mitogens and kainic acid].
The following mitogens: concanavalin A (con A), phytohemagglutinin (PHA), hydra growth factor (HGF) as well as neurotoxic agent kainic acid, caused neural differentiation (N) effects differed in value and also in character of dependence on concentration of the agent. The lowest effective concentration of con A was 75 micrograms/ml (15% neural differentiation, treatment during 3 h), and the effect reached maximum of 50-60% at 100-200 micrograms/ml. Con A concentration 50 micrograms/ml showed no effect but after 1% rabbit gamma-globulin was added, 17% neural differentiation was detected. N-effects observed after treatment of explants with con A (200 micrograms/ml, 3h) at 2 degrees and 21 degrees were similar (58 and 42% respectively). Minimum PHA concentration used (6 micrograms/ml, 18h) led to neural differentiation in 5% of explants. N-effect of PHA increased along with the concentration of the lectin and was most pronounced at 25 micrograms/ml. However, further increase in concentration (up to 200 micrograms/ml) resulted in decrease of its N-effect to 13%. At 12 micrograms/ml PHA exerted not only neural differentiation, but also lens-inducing (32%) action on the ectoderm. N-effect of HGF (2.5, 25 and 250 micrograms/ml) was lower as compared with the maximum effects of con A and PHA (30-35%). No correlation of HGF inducing action with its concentration was observed. Kainic acid showed weak N-effect (20-30%) at 1 and 10 micrograms/ml. Higher concentration (100 micrograms/ml) had no N-effect, but in 27% of explants "free" lentoids were found. Oubain (10(-3) and 10(-4) M) and HEPES (20 mM) did not affect the differentiation of explants.
Mikhaĭlov
A T
AT
Gorgoliuk
N A
NA
Bibikova
A D
AD
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004475:Ectoderm / Q000166:cytology / Q000187:drug effects*; D005775:Gastrula / Q000166:cytology / Q000187:drug effects*; D007608:Kainic Acid / Q000494:pharmacology*; D008934:Mitogens / Q000494:pharmacology*; D009417:Nerve Tissue / Q000187:drug effects* / Q000196:embryology; D009924:Organ Culture Techniques; D011896:Rana temporaria / Q000196:embryology*; D013696:Temperature; D013997:Time Factors
D008934:Mitogens; D007608:Kainic Acid
Neĭralizatsiia éksplantatov éktodermy ranneĭ gastrula Rana temporaria pod deĭstviem razlichnykh mitogenov i kainovoĭ kisloty.