title: Effect of sodium vanadate on deoxyribonucleic acid and protein syntheses in cultured rat calvariae.
abstract: Sodium vanadate, an agent known to have multiple cellular actions, was studied for its effects on aspects of bone formation in cultures of 21-day-old fetal rat calvariae. Vanadate (0.1-10 microM) stimulated the incorporation of [3H] thymidine into acid-insoluble residues (DNA); the effect appeared after 3 h and was sustained for 96 h. Vanadate increased the bone DNA content and mitotic index. Treatment with vanadate at 10 microM for 24 h or at 0.3-1 microM for 96 h increased the incorporation of [3H]proline into collagenase-digestible protein (CDP), but the effect was not specific for collagen; vanadate also increased the labeling of noncollagen protein (NCP). Vanadate increased the incorporation of [3H]proline into type I collagen without affecting other collagen types. Vanadate (100 microM) caused a marked and irreversible inhibitory effect on the labeling of DNA, CDP, and NCP. Treatment with vanadate at multiple doses for 3-96 h did not stimulate alkaline phosphatase activity, but this enzyme was inhibited in bones exposed to 1 mM vanadate for 24 h or 10 microM vanadate for 96 h. The stimulatory effect on DNA labeling was primarily observed in the periosteum, while that on CDP labeling was seen only in the periosteum-free bone. These studies indicate that sodium vanadate stimulates bone DNA, collagen, and NCP syntheses in vitro, although high doses of vanadate have an irreversible inhibitory effect.
authors.forename: E
authors.initials: E
authors.lastname: Canalis
mesh_terms: D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D003094:Collagen / Q000096:biosynthesis; D004247:DNA / Q000096:biosynthesis*; D004815:Epidermal Growth Factor / Q000494:pharmacology; D008940:Mitotic Index; D009924:Organ Culture Techniques; D010281:Parathyroid Hormone / Q000494:pharmacology; D010521:Periosteum / Q000378:metabolism; D014176:Protein Biosynthesis; D051381:Rats / Q000196:embryology; D012886:Skull / Q000166:cytology / Q000196:embryology / Q000201:enzymology / Q000378:metabolism*; D014638:Vanadates; D014639:Vanadium / Q000494:pharmacology*
chemical_list: D010281:Parathyroid Hormone; D014639:Vanadium; D014638:Vanadates; D004815:Epidermal Growth Factor; D003094:Collagen; D004247:DNA; D000469:Alkaline Phosphatase; D015056:1-Methyl-3-isobutylxanthine