title: Hamster oocyte penetration tests with oocytes frozen in propanediol: comparison with non-frozen oocytes.
abstract: Hamster oocytes were frozen using a 1,2-propanediol-sucrose procedure, which resulted in over 90% survival. After thawing and zona removal the oocytes were compared with non-frozen oocytes in a zona-free hamster egg test employing spermatozoa from human semen donors and suspected infertility patients. Similar data were obtained, indicating that propanediol-sucrose frozen hamster eggs may be used in place of fresh eggs for convenience and to avoid scheduling problems.
authors.affiliation: Reproductive and Developmental Biology, Research Institute and Gynecology, Cleveland Clinic Foundation, Ohio.
authors.forename: H H
authors.initials: HH
authors.lastname: Sachs
authors.forename: M J
authors.initials: MJ
authors.lastname: Pink
authors.forename: R B
authors.initials: RB
authors.lastname: Gwatkin
mesh_terms: D000818:Animals; D006224:Cricetinae; D005260:Female; D005615:Freezing; D008297:Male; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D011309:Preservation, Biological; D011409:Propylene Glycols; D013084:Sperm-Ovum Interactions
chemical_list: D011409:Propylene Glycols