Alteration of gastroduodenal acetylcholine metabolism induced by 2-deoxy-D-glucose in rats.
The effect of 2-Deoxy-D-glucose (2-DG) on acetylcholine (ACh) metabolism in the stomach and duodenum of rats was studied. 2-DG significantly increased [3H] choline uptake, [3H] ACh synthesis, and choline acetyltransferase and acetylcholinesterase activities in parallel with an increase in gastric acid secretion. The conversion rate of uptaken [3H] choline to [3H] ACh, the turnover rate of ACh, and the contents of ACh and choline were negligibly influenced by 2-DG. 2-Buten-4-olide, which inhibits vagus nerve activity via the central nervous system, prevented the 2-DG increased [3H] choline uptake and subsequent [3H] ACh synthesis as well as the increase in gastric acid secretion. These results suggest that neuronal activity of the vagus nerve is coupled with [3H] choline uptake and subsequent [3H] ACh synthesis, and that cholinergic neuronal activity depends on quantitative changes in ACh metabolism in the gastroduodenum.
Chaki
S
S
Department of Pharmacology, Research Center, Taisho Pharmaceutical Co., Ltd., Ohmiya, Japan.
Muramatsu
M
M
Aihara
H
H
D015107:4-Butyrolactone / Q000031:analogs & derivatives; D000109:Acetylcholine / Q000096:biosynthesis / Q000378:metabolism*; D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D002794:Choline / Q000378:metabolism; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D003837:Deoxy Sugars / Q000494:pharmacology*; D003847:Deoxyglucose / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004386:Duodenum / Q000378:metabolism*; D005663:Furans / Q000494:pharmacology; D005744:Gastric Acid / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014630:Vagus Nerve / Q000187:drug effects
D003837:Deoxy Sugars; D005663:Furans; C004511:butenolide; D003847:Deoxyglucose; D002795:Choline O-Acetyltransferase; D000110:Acetylcholinesterase; D002794:Choline; D000109:Acetylcholine; D015107:4-Butyrolactone