INCA: software for consort 30 analysis of flow cytometric calcium determinations.
The INCA program converts Consort 30-generated fluorescence list mode data collected from Indo-1-stained cells to absolute intracellular calcium concentrations (nM Ca2+i). The calcium data are plotted vs. time, allowing the user to analyze the fractions of cells responding to a given stimulus. Converted files can be restored to disk after replacing FL1 and FL2 with time and calcium, respectively, for future analysis.
Keij
J F
JF
Department of Clinical Immunology, University of Groningen, The Netherlands.
Griffioen
A W
AW
The
T H
TH
Rijkers
G T
GT
D001402:B-Lymphocytes / Q000032:analysis; D002118:Calcium / Q000032:analysis*; D001330:Electronic Data Processing; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D012984:Software; D013601:T-Lymphocytes / Q000032:analysis
D002118:Calcium