[Quantitative determination of protein in the urine by a sensitive nephelometric method].
The nephelometric method of Reiber ((1980) J. Clin. Chem. Clin. Biochem. 18, 123-127) for quantitation of proteins in cerebrospinal fluid was modified for urine. Protein concentrations between 50 and 500 mg/l (method A) or between 5 and 70 mg/l (method B) can be measured. Values greater than 150 mg/l correlate well with the Biuret- and the Coomassie method (r = 0.9804 and r = 0.9925, respectively). For lower concentrations, only albumin, determined by an enzyme-immuno-assay, gave a good correlation (r = 0.9342). The following pharmaceuticals did not influence the results: penicillin, gentamycin sulphate, dihydralazine sulphate, amoxicillin and furosemide. A reference range of 7-56 mg/24 h was established (n = 52, 90% confidence interval).
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D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007202:Indicators and Reagents; D009211:Myoglobin / Q000032:analysis; D009391:Nephelometry and Turbidimetry / Q000379:methods; D011507:Proteinuria / Q000652:urine*; D012709:Serum Albumin / Q000032:analysis; D013194:Staining and Labeling; D014168:Transferrin / Q000032:analysis
D007074:Immunoglobulin G; D007202:Indicators and Reagents; D009211:Myoglobin; D012709:Serum Albumin; D014168:Transferrin
Quantitative Proteinbestimmung im Harn mit einer empfindlichen Streulichtmethode.