[Obtaining unidirectional deletions in a plasmid using a DNA-"protector" and Bal31 exonuclease].
A method of generating unidirectional deletions from the cleavage site of any endonuclease by means of exonuclease Bal31 is developed. DNA sequence to be prevented from Bal31 hydrolysis is protected by a special "protector" element TH (Trojan Horse) inserted upstream of the sequence.
Nurminskiĭ
D I
DI
Shevelev
Iu Ia
IuIa
Kalmykova
A I
AI
D002872:Chromosome Deletion; D004247:DNA / Q000235:genetics*; D004706:Endodeoxyribonucleases; D010957:Plasmids
D004247:DNA; D004706:Endodeoxyribonucleases; C038407:exonuclease Bal 31
Poluchenie odnonapravlennykh deletsiĭ deletsiĭ v plazmide s pomoshch'iu DNK-"protektora" i ékzonukleazy Bal31.