title: Reversible acetaldehyde inhibition of A23187-stimulated amylase secretion from isolated rat pancreatic acini.
abstract: The Ca2+ ionophore, A23187, stimulated amylase secretion from isolated rat pancreatic acini in a dose-dependent manner with a maximal effect at 6 microM. Acetaldehyde, a metabolite of ethanol, caused a reduction in the magnitude of ionophore-stimulated secretion with no evidence of competitive inhibition. Furthermore, 6 microM ionophore-stimulated amylase secretion was dose-dependently inhibited by acetaldehyde. This inhibitory effect of acetaldehyde, however, was reversible on washing and reincubating acetaldehyde-treated acini. These results suggest that acetaldehyde reversibly inhibits intracellular components mediating stimulated secretion and this inhibition requires a continuous chemical interaction between acetaldehyde and intracellular component(s) regulating stimulated enzyme secretion.
authors.forename: M B
authors.initials: MB
authors.lastname: Lewin
authors.forename: A
authors.initials: A
authors.lastname: Wong
authors.forename: C W
authors.initials: CW
authors.lastname: Deveney
authors.forename: H
authors.initials: H
authors.lastname: Sankaran
mesh_terms: D000079:Acetaldehyde / Q000494:pharmacology*; D000681:Amylases / Q000378:metabolism*; D000818:Animals; D000001:Calcimycin / Q000037:antagonists & inhibitors*; D002118:Calcium / Q000502:physiology; D005260:Female; D010179:Pancreas / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012636:Secretory Rate / Q000187:drug effects
chemical_list: D000001:Calcimycin; D000681:Amylases; D000079:Acetaldehyde; D002118:Calcium