title: Flow cytometric analysis of the kinetic effects of cisplatin on lung cancer cells.
abstract: The effects of cisplatin on the cell cycle and DNA synthesis of human lung adenocarcinoma cell line PC-9 were examined by flow cytometry. The cellular DNA content and the bromodeoxyuridine (BrdUrd) incorporation rate were measured simultaneously using a monoclonal anti-BrdUrd antibody. Following exposure to cisplatin (1.0 micrograms/ml) for 1 and 24 hr, the bivariate DNA/BrdUrd distributions revealed a delayed S-phase transit and an accumulation of cells in the G2M phase. The BrdUrd-linked green fluorescence intensity continued to decrease with the lapse of time. However, early- and mid-S-phase cells soon recovered DNA synthesis activity, and the former showed higher activity than the control cells. These findings suggested the vigorous DNA synthesis of cells in early S phase. However, for quantitative analysis of chemotherapeutic effects, some problems remained to be resolved regarding the condition for DNA denaturation and its alteration by the agents.
authors.affiliation: First Department of Internal Medicine, Asahikawa Medical College, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Fujikane
authors.forename: T
authors.initials: T
authors.lastname: Shimizu
authors.forename: T
authors.initials: T
authors.lastname: Tsuji
authors.forename: S
authors.initials: S
authors.lastname: Ishida
authors.forename: Y
authors.initials: Y
authors.lastname: Ohsaki
authors.forename: S
authors.initials: S
authors.lastname: Onodera
mesh_terms: D000230:Adenocarcinoma / Q000032:analysis / Q000378:metabolism / Q000473:pathology*; D001973:Bromodeoxyuridine / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects* / Q000473:pathology; D002945:Cisplatin / Q000494:pharmacology*; D004273:DNA, Neoplasm / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007700:Kinetics; D008175:Lung Neoplasms / Q000032:analysis / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000032:analysis / Q000378:metabolism / Q000473:pathology
chemical_list: D004273:DNA, Neoplasm; D001973:Bromodeoxyuridine; D002945:Cisplatin