Differentiation between Trichinella spiralis and T. pseudospiralis infective larvae by a monoclonal antibody.
Crude saline extracts of Trichinella spiralis and T. pseudospiralis infective larvae were studied by Western blot analysis using a monoclonal antibody, named ES/TA2 and produced against T. spiralis larvae. This monoclonal antibody recognized seven major antigenic components in T. spiralis larvae with apparent Mr: 45, 48, 50, 68, 70, 92 and 105 kDa and five in T. pseudospiralis larvae: 38, 50, 70, 72 and 92 kDa. SDS-PAGE of both extracts did not reveal appreciable differences in the range of molecular weights recognized by ES/TA2. These facts show the existence of immunological differences among proteins with apparently identical molecular weights.
Rodriguez-Perez
J
J
Instituto López-Neyra de Parasitología, Unidad de Inmunología, Granada, Spain.
Gomez-Garcia
V
V
Rodriguez-Osorio
M
M
Rojas-Gonzalez
J
J
Gomez-Morales
M A
MA
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000947:Antigens, Helminth / Q000032:analysis*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D007814:Larva / Q000276:immunology / Q000302:isolation & purification; D008970:Molecular Weight; D014234:Trichinella / Q000276:immunology / Q000302:isolation & purification*
D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth