The determination of protein, oligonucleotide and peptide molecular weights by ion-spray mass spectrometry.
The mass spectra of several compounds with molecular weights in the 2500-20,000 Da range were obtained with a quadrupole mass spectrometer equipped with an atmospheric pressure ion source. Average molecular weight determinations of mellitin (2846.4 Da), a synthetic oligonucleotide (4262.8 Da), myoglobin (16,950.4 Da) and on the subunits of beta-lactoglobulin (18,277.1 Da) requiring as little as 1 pmol of material were achieved with accuracies and precisions of +/- 1 Da. An ion-spray interface was used to produce ions via the ion evaporation process, producing mass spectra containing a series of multiply-charged molecular species. A simple method for calculating the molecular weight of unknown compounds from the spectra containing multiply-charged ions is described.
Covey
T R
TR
Sciex, Division of MDS Health Group Ltd, Ontario, Canada.
Bonner
R F
RF
Shushan
B I
BI
Henion
J
J
D007782:Lactoglobulins / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D008555:Melitten / Q000737:chemistry*; D008970:Molecular Weight; D009211:Myoglobin / Q000737:chemistry*
D007782:Lactoglobulins; D009211:Myoglobin; D008555:Melitten