Vaccination against the intracellular bacterium Listeria monocytogenes with a clonotypic antiserum.
The T cell clone 26.1.1, which confers specific protection against the intracellular bacterium Listeria monocytogenes, was fused to BW 5147. The resulting T cell hybridoma, TLm1, could be stimulated to secret interleukin 2 by antigen plus accessory cells or concanavalin A. Stimulation was specific for an epitope expressed by L. monocytogenes EGD but not ATCC 19114 and was H-2I-A restricted. Antisera against TLm1 were raised in syngeneic mice and tested for their capacity to block TLm1 responses. Two antisera were identified that blocked antigen but not concanavalin A stimulation of TLm1 and did not affect antigen stimulation of similar but not identical L. monocytogenes-specific T cell hybridomas. Hence, these antisera had clonotypic activity. When these antisera were administered subcutaneously in complete Freund's adjuvant, mice were protected against a subsequent L. monocytogenes infection. Protection was antigen specific and H-2 nonrestricted. These findings suggest the feasibility of clonotypic antibodies for vaccination against intracellular bacterial infections.
Kaufmann
S H
SH
Eichmann
K
K
Müller
I
I
Wrazel
L J
LJ
D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics; D001428:Bacterial Vaccines / Q000008:administration & dosage*; D001667:Binding, Competitive; D002999:Clone Cells / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D007106:Immune Sera / Q000008:administration & dosage* / Q000032:analysis / Q000494:pharmacology; D008089:Listeria monocytogenes / Q000276:immunology; D008088:Listeriosis / Q000276:immunology / Q000517:prevention & control*; D008297:Male; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology*
D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D000939:Epitopes; D007106:Immune Sera