Serum platelet-activating factor acetylhydrolase activity in rats with gastric ulcers induced by water-immersion stress.
Platelet-activating factor (PAF) acetylhydrolase is an enzyme which hydrolyzes PAF to yield inactive lysoPAF. This study focused on the influence of water-immersion stress on serum PAF acetylhydrolase activity. The enzyme activity was determined by measurement of [3H]acetate produced from 1-O-alkyl-2-[3H]acetyl-sn-glycero-3-phosphocholine upon precipitation of the complex of the radioactive substrate and albumin with trichloroacetic acid. The onset of water-immersion stress caused the development of gastric lesions associated with a significant increase in serum PAF acetylhydrolase activity. Serum PAF acetylhydrolase may leak into the blood from some tissues in rats with gastric injury induced by water-immersion stress and might control the action of PAF.
Fujimura
K
K
Third Department of Internal Medicine, Kansai Medical School, Osaka, Japan.
Sugatani
J
J
Miwa
M
M
Mizuno
T
T
Sameshima
Y
Y
Saito
K
K
D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; D000818:Animals; D007101:Immersion; D008297:Male; D010740:Phospholipases / Q000097:blood*; D010741:Phospholipases A / Q000097:blood*; D051381:Rats; D011919:Rats, Inbred Strains; D013270:Stomach / Q000473:pathology; D013276:Stomach Ulcer / Q000201:enzymology* / Q000473:pathology; D013312:Stress, Physiological / Q000097:blood* / Q000209:etiology; D013997:Time Factors
D010740:Phospholipases; D010741:Phospholipases A; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase