The permeability of lysosomes to sugars. Effect of diethylstilbestrol on the osmotic activation of lysosomes induced by glucose.
We have investigated the effect on the osmotic activation of rat liver lysosomes, by glucose penetration, of different substances known to inhibit the glucose transport through the plasma membrane. Diethylstilbestrol is the most efficient, particularly when purified lysosomes are used. It has no effect on osmotic activation induced by hypo-osmotic sucrose or by iso-osmotic KCl. It is proposed that diethylstilbestrol reacts with specific sites involved in the glucose translocation through the lysosomal membrane. These sites could not be identified by binding experiments, presumably owing to the considerable unspecific binding of the compound to the membrane.
Jadot
M
M
Laboratoire de Chimie Physiologique, Facultés Universitaires Notre Dame de la Paix, Namur, Belgium.
Wattiaux-De Coninck
S
S
Wattiaux
R
R
D000118:Acetylglucosaminidase / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D001693:Biological Transport, Active / Q000187:drug effects; D020789:Cathepsin C; D004054:Diethylstilbestrol / Q000494:pharmacology*; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008247:Lysosomes / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009995:Osmosis / Q000187:drug effects; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D011189:Potassium Chloride; D004054:Diethylstilbestrol; D000118:Acetylglucosaminidase; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D020789:Cathepsin C; D005947:Glucose