title: [Colorimetric determination of isoniazid in aqueous solutions and biological fluids].
abstract: Method has been developed for determination of tuberculostatic isoniazid in aqueous solutions and biological fluids. The principle of the method is that hydrazine group of isoniazid reacts with 1-fluoro-2,4-dinitrobenzene forming a red chromophor in alkaline medium. The absorbency maximum of the chromophor is at 530 nm. Biological fluids were deproteinized and diluted with acetonitrile then adjusted to pH = 8.2 with trometamol-hydrochloric acid buffer and reacted with 1-fluoro-2,4-dinitrobenzene dissolved in acetonitrile. The chromphore was measured colorimetricaly against a blank solution which did not contain isoniazid. The method was calibrated in the range of 1-30 mg/l isoniazid and reproducibility was checked with recovery % determinations.
authors.forename: A
authors.initials: A
authors.lastname: Csiba
mesh_terms: D001826:Body Fluids / Q000032:analysis*; D003124:Colorimetry / Q000379:methods; D006801:Humans; D007538:Isoniazid / Q000032:analysis* / Q000097:blood; D012996:Solutions
chemical_list: D012996:Solutions; D007538:Isoniazid
vernacular_title: Kolorimetriás módszer izoniazid /INH/ meghatározására vizes oldatából és biológiai folyadékokból.