[Effect of adriamycin on cell cycle by bromodeoxyuridine-labeling method].
We studied the effect of adriamycin on DNA synthesizing cells from the view point of cell cycle. DNA synthesizing cells were marked by bromodeoxyuridine, which is a known marker for such cells, before administration of adriamycin. Then, the level of this marker was sequentially measured by flow cytometry. At the low concentration (0.01 micrograms/ml), adriamycin caused delay of the shifting time to the S phase 16 hours after administration. The marked cells were accumulated at the G2M phase at a moderate concentration (0.1 micrograms/ml), and blocked at the S phase at the higher concentration (1.0 micrograms/ml).
Tenjin
T
T
2nd Dept. of Surgery, Nippon Medical School.
Kimura
T
T
Takeda
H
H
Sakagami
S
S
Takahashi
N
N
Shoji
T
T
Asano
G
G
D001973:Bromodeoxyuridine; D002453:Cell Cycle / Q000187:drug effects; D004273:DNA, Neoplasm / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000008:administration & dosage / Q000494:pharmacology*; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D007399:Interphase / Q000187:drug effects
D004273:DNA, Neoplasm; D004317:Doxorubicin; D001973:Bromodeoxyuridine