title: Differences in chlorophyll-protein complexes and composition of polypeptides between thylakoids from bundle sheaths and mesophyll cells in maize.
abstract: Thylakoids from enzymatically separated bundle sheath and mesophyll tissue chloroplasts were examined for their chlorophyll-proteins by tube sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS-PAGE). Differences were found in distribution of chlorophyll among peaks. The chlorophyll-protein a peak (CPa), considered to be the photosystem II (PSII) reaction centre by many authors, was seen to be absent in bundle sheath thylakoid samples. The slab SDS-PAGE revealed the absence of the polypeptides present in PSII preparations of chloroplast subfractions having only PSII activity. This finding confirms Anderson's hypothesis of the structure of grana and stroma thylakoids.
authors.forename: R
authors.initials: R
authors.lastname: Bassi
authors.forename: A
authors.initials: A
authors.lastname: dal Belin Peruffo
authors.forename: R
authors.initials: R
authors.lastname: Barbato
authors.forename: R
authors.initials: R
authors.lastname: Ghisi
mesh_terms: D055598:Chemical Phenomena; D002621:Chemistry; D002734:Chlorophyll / Q000032:analysis*; D002736:Chloroplasts / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D010084:Oxidation-Reduction; D010455:Peptides / Q000032:analysis; D010940:Plant Proteins / Q000032:analysis*; D013194:Staining and Labeling; D003313:Zea mays / Q000032:analysis* / Q000648:ultrastructure
chemical_list: D010455:Peptides; D010940:Plant Proteins; D002734:Chlorophyll