In vivo chemotaxis of oxidised lipids: 1. Chemotactic effects of oxidised arachidonic acid on rat leucocytes.
In vivo chemotaxis to rat leucocytes was shown to mildly oxidised arachidonic acid in the migration into cotton pellet method. Amounts of oxidised acid, 0.25, 0.5 and 2.5 mg, mobilized net leucocytes (mostly neutrophils) into experimental pellets in 12 h. The 0.5 mg of oxidised acid showed maximum chemotactic activity and attracted maximum net leucocytes into the experimental pellets at 12 h. Doses lower or higher than this were less effective. Equivalent doses of pure arachidonic acid failed to show these chemotactic activities but evoked inflammatory reactions at the experimental pellet sites. Chemotactic activity seemed therefore to require mild oxidation of the lipid. Oxidation also seemed to stimulate spontaneous migratory activity into the control pellets.
Onyia
K A
KA
Department of Physiology, School of Medicine, College of Medical Sciences, University of Benin, Nigeria.
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism*; D002634:Chemotaxis, Leukocyte; D007958:Leukocyte Count; D008297:Male; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains
D001095:Arachidonic Acids