[Isolation of alpha-latrotoxin from karakurt spider venom using monoclonal antibodies].
A method of alpha-latrotoxin (LT) isolation from the venom of Latrodectus mactans tredecimguttatus by means of immunoaffinity chromatography on sepharose conjugated with monoclonal antibodies against LT has been developed. This one-step, high-yield, relatively simple and rapid procedure yields active LT for structural and functional studies of its receptor.
Pashkov
V N
VN
Kovalevskaia
G I
GI
Krasnoperov
V G
VG
Bulgakov
O V
OV
D000911:Antibodies, Monoclonal; D001180:Arthropod Venoms / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D013111:Spider Venoms / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D001180:Arthropod Venoms; D013111:Spider Venoms; C020763:alpha-latrotoxin
Vydelenie alpha-latrotoksina iz iada pauka karakurta s pomoshchiu monoklonalnykh antitel.