Biogenic amines and active peptides in extracts of the skin of thirty-two European amphibian species.
1. Extracts prepared from fresh or dried skins of 32 European amphibian species were submitted to chemical (colour reactions) and biological screening to determine the occurrence and contents of biogenic amines and peptides active on smooth muscle preparations and blood pressure. 2. Only indolealkylamines were detectable in the skins. They were represented by tryptamine, 5-hydroxytryptamine, and its N-methylated, cyclized and sulphoconjugated derivatives. 3. The peptide families identified in the extracts were as follows: bombesins (bombesin and alytesin), bradykinins (bradykinin, bradykinin 1-8, bradykinin 1-7), chemotactic peptides (RECP I, II and III), bombinins and TRH. Bombesins, bombinins and TRH (thyrotropin-releasing hormone) were isolated from skin extracts of discoglossid frogs; chemotactic peptides and again TRH from extracts of ranid frogs. 4. Further research will certainly lengthen the list of active peptides in the skin of European amphibians, as is the case with Australian, American and African amphibians.
Roseghini
M
M
Department of Biochemical Sciences, University La Sapienza, Rome, Italy.
Falconieri Erspamer
G
G
Severini
C
C
Simmaco
M
M
D000595:Amino Acid Sequence; D000663:Amphibians / Q000378:metabolism*; D000818:Animals; D001679:Biogenic Amines / Q000032:analysis*; D001681:Biological Assay; D002025:Bufonidae; D002853:Chromatography, Liquid; D003124:Colorimetry; D005060:Europe; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis*; D012867:Skin / Q000032:analysis*
D001679:Biogenic Amines; D010455:Peptides