In vivo priming of virus-specific cytotoxic T lymphocytes with synthetic lipopeptide vaccine.
Cytotoxic T lymphocytes (CTL) constitute an essential part of the immune response against viral infections. Such CTL recognize peptides derived from viral proteins together with major histocompatibility complex (MHC) class I molecules on the surface of infected cells, and usually require in vivo priming with infectious virus. Here we report that synthetic viral peptides covalently linked to tripalmitoyl-S-glycerylcysteinyl-seryl-serine (P3CSS) can efficiently prime influenza-virus-specific CTL in vivo. These lipopeptides are able to induce the same high-affinity CTL as does the infectious virus. Our data are not only relevant to vaccine development, but also have a bearing on basic immune processes leading to the transition of virgin T cells to activated effector cells in vivo, and to antigen presentation by MHC class I molecules.
Deres
K
K
Institut für Organische Chemie, Universität Tübingen.
Schild
H
H
Wiesmüller
K H
KH
Jung
G
G
Rammensee
H G
HG
D000595:Amino Acid Sequence; D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D006183:H-2 Antigens / Q000276:immunology; D008074:Lipoproteins / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014612:Vaccines / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology*; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
D006183:H-2 Antigens; D008074:Lipoproteins; D010446:Peptide Fragments; D014612:Vaccines; D014614:Vaccines, Synthetic; D014764:Viral Proteins; D014765:Viral Vaccines; C061893:N-palmitoyl-5,6-dipalmitoyl-S-glycerylcysteinyl-seryl-serine