Effects of okadaic acid on mouse hemopoietic cells.
Effects of okadaic acid, a potent non-12-O-tetradecanoyl-phorbol-13-acetate(TPA)-type tumor promoter, on mouse hemopoietic cells were investigated. Okadaic acid stimulated mouse bone marrow cells to form granulocyte-macrophage colony-forming unit (CFU-GM) colonies without added colony stimulating factors(CSFs). At the concentration of 1.82 x 10(-8) M, colony formation of 77 +/- 14 colonies/1 x 10(5) bone marrow cells was observed. Observations on the effects of other cells on the CSF induction suggested that okadaic acid primarily stimulated the functions of macrophages, and the CSF production from macrophages might be attributed to the CFU-GM colony formation. On the other hand, the erythroid colony-forming unit(CFU-E) colony formation stimulated by
Oka
S
S
Fermentation Research Institute, Agency of Industrial Science and Technology, Ibaraki, Japan.
Nakagomi
K
K
Tanaka
H
H
Yasumoto
T
T
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects; D002273:Carcinogens / Q000494:pharmacology*; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000096:biosynthesis; D004988:Ethers, Cyclic / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D007700:Kinetics; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D019319:Okadaic Acid; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects
D002273:Carcinogens; D003115:Colony-Stimulating Factors; D004988:Ethers, Cyclic; D019319:Okadaic Acid