title: Uptake of amino acids and ammonia at mid-gestation by the fetal lamb.
abstract: Fetal uptakes of amino acids and ammonia via the umbilical circulation were measured in single pregnant ewes at mid-gestation (range 66-81 days). There were significant net fluxes from placenta to fetus of ammonia and twelve amino acids (in decreasing order: glutamine, glycine, alanine, proline, lysine, arginine, threonine, valine, leucine, tyrosine, asparagine, isoleucine) and net fluxes from fetus to placenta of glutamate and serine. The estimated serine flux was 139 mumol day-1 (g fetal dry wt)-1. Comparison with late gestation data indicated a similar pattern of amino acid exchange. However, the relatively large placental uptake of fetal serine was a distinctive feature of mid-gestation. The net fetal uptake of amino acid nitrogen was 2.83 +/- 0.66 micrograms N (mumol O2 uptake)-1, or 18 mg N day-1 (g dry wt)-1. This uptake was similar in magnitude to the combined fetal requirements for nitrogen accretion and urea synthesis and represented approximately 32 and 43% of fetal carbon and energy requirements, respectively.
authors.affiliation: Department of Pediatrics, University of Colorado School of Medicine, Denver 80262.
authors.forename: A W
authors.initials: AW
authors.lastname: Bell
authors.forename: J M
authors.initials: JM
authors.lastname: Kennaugh
authors.forename: F C
authors.initials: FC
authors.lastname: Battaglia
authors.forename: G
authors.initials: G
authors.lastname: Meschia
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D000641:Ammonia / Q000378:metabolism*; D000818:Animals; D001693:Biological Transport, Active; D002244:Carbon / Q000378:metabolism; D004734:Energy Metabolism; D005260:Female; D005333:Fetus / Q000378:metabolism*; D005865:Gestational Age; D009584:Nitrogen / Q000378:metabolism; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D012756:Sheep
chemical_list: D000596:Amino Acids; D002244:Carbon; D000641:Ammonia; D009584:Nitrogen