Monospecific antibodies against a synthetic peptide predicted from the alpha-3 nicotinic receptor cDNA inhibit binding of [3H]nicotine to rat brain nicotinic cholinergic receptor.
Polyclonal antibodies were raised against a synthetic decapeptide (designated S3) predicted from a segment of the alpha-3 subunit cDNA (amino acid residues 130-139) encoding the rat brain nicotinic cholinergic receptor. This segment was selected because it may be proximate to the nicotine/acetylcholine-binding site of the receptor (1). By radioligand binding assays and sucrose density gradient centrifugation, these monospecific antibodies were shown to inhibit the binding of [3H]nicotine to both the large molecular weight rat brain receptor (240 kDa) and to an SDS-disaggregated nicotine-binding subunit species (80 kDa), in a dose-dependent manner. The neutralizing effect of the anti-S3 antibodies supports the view that this region of the protein is closely related to the agonist binding site.
Madhok
T C
TC
Endocrine-Neuroscience Laboratory, Minneapolis Medical Research Foundation, MN.
Chao
C C
CC
Matta
S G
SG
Hong
A
A
Sharp
B M
BM
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001921:Brain / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D004247:DNA / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D007700:Kinetics; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009538:Nicotine / Q000378:metabolism*; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000276:immunology; D051381:Rats; D011978:Receptors, Nicotinic / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D046911:Macromolecular Substances; D009842:Oligopeptides; D011978:Receptors, Nicotinic; D009538:Nicotine; D004247:DNA