title: Gamma-glutamyltransferase: evaluation of a new method.
abstract: The determination of gamma-glutamyltransferase using a new donor substrate, L-gamma-glutamyl-3,5-dibromo-4-hydroxyanilide, was evaluated in two laboratories. Between-run and within-run precision are excellent and comparable with those of other methods for the determination of gamma-glutamyltransferase. The activity concentrations of gamma-glutamyltransferase from human sera obtained with the new donor substrate were slightly higher than those obtained with L-gamma-glutamyl-4-nitroanilide. The reverse was found for sera enriched with gamma-glutamyltransferase of animal origin. Temperature conversion factors (activity 37 degrees C/activity 30 degrees C) are given. The advantages of this method are discussed. The new assay appears to provide a good alternative for the method with L-gamma-glutamyl-4-nitroanilide or one of its derivatives as substrate.
authors.affiliation: Universiteitskliniek voor kinderen en jeugdigen, Het Wilhelmina Kinderziekenhuis, Utrecht.
authors.forename: C
authors.initials: C
authors.lastname: van der Heiden
authors.forename: J C
authors.initials: JC
authors.lastname: Hafkenscheid
authors.forename: J
authors.initials: J
authors.lastname: van der Ven-Jongekrijg
authors.forename: A
authors.initials: A
authors.lastname: van der Stokker
authors.forename: C
authors.initials: C
authors.lastname: van Rooijen
mesh_terms: D000818:Animals; D002863:Chromogenic Compounds; D005971:Glutamates; D006801:Humans; D008722:Methods; D005723:gamma-Glutamyltransferase / Q000097:blood*
chemical_list: D002863:Chromogenic Compounds; D005971:Glutamates; D005723:gamma-Glutamyltransferase