Regulation of bone sialoprotein mRNA by steroid hormones.
In this report we demonstrate an increase in the steady-state level of bone sialoprotein (BSP) mRNA in rat calvaria and a rat osteosarcoma cell line (ROS 17/2.8) after treatment with the synthetic glucocorticoid, dexamethasone. In contrast, 1.25-dihydroxyvitamin D3 reduced the amount of BSP mRNA in calvaria and inhibited the dexamethasone induction in ROS 17/2.8 cells. The increase in BSP mRNA is most likely due to an increase in the transcriptional rate. The stability of mRNA was unchanged after dexamethasone treatment with a half-life of approximately 5 h. Nuclear transcription experiments with nuclei isolated from ROS 17/2.8 cells showed an increased BSP mRNA synthesis in cells treated with dexamethasone.
Oldberg
A
A
Department of Medical and Physiological Chemistry, University of Lund, Sweden.
Jirskog-Hed
B
B
Axelsson
S
S
Heinegård
D
D
D000818:Animals; D000831:Animals, Newborn; D001842:Bone and Bones / Q000187:drug effects / Q000378:metabolism*; D002117:Calcitriol / Q000494:pharmacology; D002460:Cell Line; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D005796:Genes / Q000187:drug effects*; D058574:Integrin-Binding Sialoprotein; D012516:Osteosarcoma; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics*; D051381:Rats; D012795:Sialoglycoproteins / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects*
C546873:Ibsp protein, rat; D058574:Integrin-Binding Sialoprotein; D012333:RNA, Messenger; D012795:Sialoglycoproteins; D003907:Dexamethasone; D002117:Calcitriol