[Development of a diagnostic test system for detecting soluble staphylococcal antigens by an immunoenzyme method].
ELISA is used for detecting the soluble staphylococcal antigen in patients with purulent septic infections. The optimum conditions for the assay have been established: the dose of staphylococcal gamma globulin for plate sensitization should be 5.0-10.0 micrograms/ml, the pH of the buffer solution 9.6-10.0, the time and temperature of incubation 18-20 hours at 4 degrees C or 5 hours at 37 degrees C. The possibility of using plates manufactured in the USSR has been shown. The sensitivity of the above diagnostic test system is 0.005 microgram/ml.
Gavriliaka
R D
RD
Semina
N A
NA
Fiks
L I
LI
Shabalina
S V
SV
Tsvetkova
N V
NV
D000942:Antigens, Bacterial / Q000032:analysis*; D002021:Buffers; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes / Q000032:analysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007124:Immunoenzyme Techniques / Q000295:instrumentation; D012995:Solubility; D013203:Staphylococcal Infections / Q000175:diagnosis*; D013210:Staphylococcus / Q000276:immunology*; D013696:Temperature; D013997:Time Factors
D000942:Antigens, Bacterial; D002021:Buffers; D000939:Epitopes
Razrabotka diagnosticheskoĭ test-sistemy dlia vyiavleniia rastvorimykh stafilokokkovykh antigenov immunofermentnym metodom.