A new isoschizomer, BnaI, of the BamHI restriction endonuclease.
By assaying the yield of phage SPO1 we have identified a new restriction-modification activity in the Bacillus natto B3364 strain. A class II restriction endonuclease, BnaI, isolated from the crude extract of B3364 cells was shown to be a true isoschizomer of the BamHI endonuclease. The Mr, stability and optimal conditions required for DNA digestion were determined for BnaI. Although both enzymes show the same specificity, BnaI and BamHI differ from each other in all the properties specified above.
Kim
E L
EL
Institute of Molecular Biology and Genetics, Kiev, USSR.
Maliuta
S S
SS
D001407:Bacillus / Q000201:enzymology*; D001665:Binding Sites; D015245:Deoxyribonuclease BamHI / Q000302:isolation & purification / Q000378:metabolism*; D005990:Glycerol / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism; D008970:Molecular Weight; D012965:Sodium Chloride; D013696:Temperature
D007527:Isoenzymes; D012965:Sodium Chloride; D015245:Deoxyribonuclease BamHI; D005990:Glycerol