title: B cell activation: role of dendritic and T cell factors in the response to thymic-independent and -dependent antigens.
abstract: We described a cloned dendritic cell, clone Den-1, that is a potent accessory cell for some B cell responses. Clone Den-1 produces a novel lymphokine that is distinct from previously described factors produced by T cells. In the present study, we compare the role of nonspecific helper factors produced by Den-1 (Den-1 SN) or the T cell thymoma EL4 (EL4-SN) in promotion of B cell plaque-forming cell (PFC) responses to a variety of antigens. We find that the antigen in culture determines the B cell requirement for dendritic and/or T cell factors. B cell PFC responses to TNP-Brucella abortus (BA) and TNP-lipopolysaccharide (LPS) are greatly increased by EL4-SN but show little, if any, enhancement with Den-1 SN. Responses to TNP-polyacrylamide are reconstituted by either Den-1 SN or EL4-SN, whereas responses to TNP-Ficoll, TNP-dextran and TNP-levan are reconstituted by Den-1 SN and are much less sensitive to factors present in EL4-SN. Responses to SRBC require the presence of both Den-1 SN and EL4-SN. We also show that the time at which Den-1 SN must be provided to the B cell is dependent on the antigen in culture. Our findings are discussed in terms of present classification of antigens based on their ability to stimulate various B cell subpopulations.
authors.forename: R
authors.initials: R
authors.lastname: DeKruyff
authors.forename: C
authors.initials: C
authors.lastname: Clayberger
authors.forename: R
authors.initials: R
authors.lastname: Fay
authors.forename: H
authors.initials: H
authors.lastname: Cantor
mesh_terms: D000180:Acrylic Resins / Q000276:immunology; D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D000955:Antigens, T-Independent / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D002003:Brucella abortus / Q000276:immunology; D002999:Clone Cells / Q000276:immunology / Q000378:metabolism; D003911:Dextrans / Q000276:immunology; D005362:Ficoll / Q000031:analogs & derivatives / Q000276:immunology; D006133:Growth Substances / Q000502:physiology; D006462:Hemolytic Plaque Technique; D007376:Interleukin-2 / Q000502:physiology; D015847:Interleukin-4; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors; D014301:Trinitrobenzenes / Q000276:immunology; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D000955:Antigens, T-Independent; D003911:Dextrans; D005362:Ficoll; D006133:Growth Substances; D007376:Interleukin-2; D015847:Interleukin-4; D008222:Lymphokines; D014301:Trinitrobenzenes; C020529:TNP-ficoll; C028795:trinitrophenyl polyacrylamide; D000099166:Polyacrylamides