Characterization of monoclonal antibodies to the IGF-I receptor that inhibit IGF-I binding to human cells.
A mouse monoclonal antibody directed against the human placental IGF-I receptor is shown to completely inhibit IGF-I binding to both human placental membranes and circulating human mononuclear leukocytes. Insulin binding is unaffected. Studies with 125I-labelled anti-receptor antibody indicate that the antibody reacts with an epitope distinct from the IGF-I binding site. These antibodies will be powerful probes of IGF-I binding and action in human cells.
Flier
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JS
Moses
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000502:physiology; D001667:Binding, Competitive; D000939:Epitopes / Q000276:immunology; D005260:Female; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D051379:Mice; D009000:Monocytes / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011956:Receptors, Cell Surface / Q000276:immunology* / Q000378:metabolism; D017451:Receptors, Somatomedin; D013002:Somatomedins / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007328:Insulin; D010455:Peptides; D011956:Receptors, Cell Surface; D017451:Receptors, Somatomedin; D013002:Somatomedins