Homologous monoclonal antibodies specific for alpha 1-3 dextran stimulate polyclonal proliferation of Lyb-5+ B cells.
In this paper we describe the polyclonal blast response induced in nonimmune murine splenocytes by two homologous alpha 1-3 dextran-binding myeloma protein, J558 and MOPC104E. This stimulation appears to be independent of MHC or IgCh gene complex control, and proceeds entirely in a T-dependent fashion. The responding B cell population appears to belong to the more mature Lyb-5+ subset. This response was elicited by several independently prepared batches of J558 and MOPC104E, each of which was conclusively shown to be free of endotoxin contamination. Experiments are presented that suggest that this stimulation is being mediated via the Fab portion of these two myeloma proteins, in particular, a shared IdX, rather than through their Fc.
Victor-Kobrin
C
C
Bikoff
E K
EK
Bona
C A
CA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology*; D000918:Antibody Specificity; D000950:Antigens, Ly / Q000235:genetics; D000955:Antigens, T-Independent / Q000276:immunology; D001402:B-Lymphocytes / Q000145:classification* / Q000276:immunology; D001666:Binding Sites, Antibody; D003911:Dextrans / Q000276:immunology* / Q000378:metabolism; D004731:Endotoxins / Q000494:pharmacology; D005260:Female; D007153:Immunologic Deficiency Syndromes / Q000235:genetics; D007399:Interphase; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008819:Mice, Nude; D009194:Myeloma Proteins / Q000276:immunology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000950:Antigens, Ly; D000955:Antigens, T-Independent; D003911:Dextrans; D004731:Endotoxins; D009194:Myeloma Proteins; C040834:alpha-1,3-dextran