Accumulation of autolysosomes after receptor-mediated introduction of Ep459-asialofetuin conjugate into lysosomes in rat hepatocytes.
Administration of Ep459-asialofetuin conjugate (Ep459-AF) and pepstatin-asialofetuin conjugate (Ps-AF) to rats effectively inhibited lysosomal BANA hydrolase and cathepsin D in the liver, respectively, at a very low dose. Ep459-AF treatment also led to an accumulation of autolysosomes in rat liver. There was a close correlation between the accumulation of autolysosomes and the inhibition of BANA hydrolase activity. However, as opposed to the inhibition of thiol proteases, the inhibition of cathespin D did not cause accumulation of autolysosomes in the rat liver. These results suggest that autophagy in rat hepatocytes is a common occurrence under normal physiological conditions and that thiol proteases are digestive enzymes essential for the autolysomes.
Furuno
K
K
Miwa
N
N
Ishikawa
T
T
Kato
K
K
D000626:Aminopeptidases / Q000378:metabolism*; D000818:Animals; D001212:Asialoglycoproteins; D002402:Cathepsin D / Q000037:antagonists & inhibitors; D056655:Cathepsin H; D002403:Cathepsins; D002458:Cell Fractionation; D003546:Cysteine Endopeptidases; D066298:In Vitro Techniques; D007700:Kinetics; D007930:Leucine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D008099:Liver / Q000378:metabolism*; D008247:Lysosomes / Q000378:metabolism* / Q000648:ultrastructure; D008854:Microscopy, Electron; D010436:Pepstatins / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D011955:Receptors, Drug / Q000378:metabolism; D000509:alpha-Fetoproteins / Q000378:metabolism* / Q000494:pharmacology
D001212:Asialoglycoproteins; C043869:Ep 459-asialofetuin conjugate; D010436:Pepstatins; D011955:Receptors, Drug; D000509:alpha-Fetoproteins; C014195:Streptomyces pepsin inhibitor; D002403:Cathepsins; D000626:Aminopeptidases; D003546:Cysteine Endopeptidases; D056655:Cathepsin H; C534128:Ctsh protein, rat; D002402:Cathepsin D; D007930:Leucine; C031375:pepstatin