Metabolism of thromboxane B2 in the isolated perfused rat kidney: mass spectrometric identification of urinary products.
The metabolism of thromboxane B2 (TXB2), the stable breakdown product of thromboxane A2, has been studied in isolated perfused kidney preparations using a recirculating system. In a first experiment, TXB2 was infused at a rate of 20 micrograms/kg per min. In a second experiment, a 1:1 mixture of TXB2 and octadeuterated TXB2 (0.4 microgram/kg per min each) was infused. Urinary samples collected during the infusion of TXB2 or vehicle were extracted on C18 cartridges and derivatized to methyl or pentafluorobenzyl ester, methyloxime, trimethylsilyl ether. Samples were analyzed by high-resolution gas chromatography-mass spectrometry in the electron impact and negative ion chemical ionization modes. Products of beta-oxidation, reduction of the delta 5,6 double bond and dehydrogenation at C-11 (2,3-dinor-TXB2, 2,3-dinor-TXB1, 2,3,4,5-tetranor-TXB1 and 11-dehydro-TXB2) were identified in addition to unmetabolized TXB2. 2,3,4,5-tetranor-TXB1 and 2,3-dinor-TXB1 were the most abundant metabolites.
Chiabrando
C
C
Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.
Pinciroli
V
V
Perico
N
N
Campoleoni
A
A
Benigni
A
A
Remuzzi
G
G
Fanelli
R
R
D000818:Animals; D003903:Deuterium; D008401:Gas Chromatography-Mass Spectrometry; D007668:Kidney / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry; D015394:Molecular Structure; D051381:Rats; D011919:Rats, Inbred Strains; D013929:Thromboxane B2 / Q000652:urine*
D013929:Thromboxane B2; D003903:Deuterium