Quantitation of metabolic cooperation by measurement of HGPRTase activity.
A method for the quantitation of metabolic cooperation between cells is described. The method depends upon measuring the increase in HGPRTase activity that occurs between HGPRT+ cells and the HGPRT-LN (Lesch-Nyhan) cells. The variables upon which this method depends and their effect on the final determination are discussed.
Vitkauskas
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Canellakis
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D000818:Animals; D001345:Autoradiography; D002450:Cell Communication; D002460:Cell Line; D006801:Humans; D019271:Hypoxanthine; D007041:Hypoxanthine Phosphoribosyltransferase / Q000378:metabolism*; D007042:Hypoxanthines / Q000378:metabolism; D008722:Methods; D051379:Mice; D010754:Phosphoribosyl Pyrophosphate / Q000378:metabolism
D007042:Hypoxanthines; D019271:Hypoxanthine; D010754:Phosphoribosyl Pyrophosphate; D007041:Hypoxanthine Phosphoribosyltransferase