Genetic studies of low-abundance human plasma proteins. XII. A new variant of corticosteroid-binding globulin detected by isoelectric focusing/immunoblotting.
Thin-layer polyacrylamide gel isoelectric focusing over the pH range 3.5-5 followed by immunoblotting was used to investigate the occurrence and frequency of genetic variation in corticosteroid-binding globulin (CBG). Plasma samples from US Caucasians (n = 105) and US Blacks (n = 106) from Pittsburgh, Pa., Canadian Indians from Vancouver Island (n = 91) and Nigerian Blacks (n = 116) were analyzed. A complex isoprotein pattern was observed in all individuals tested. Reduction of this pattern to a single primary band following neuraminidase treatment indicates that the observed intraindividual variation is due to variation in the number of sialic acid residues associated with CBG. The CBG variant pattern consisted of a series of isoprotein bands having the same mobility as the common pattern, and a second series of bands at a more acidic isoelectric point. This pattern is consistent with heterozygosity for a rare CBG allele.
Eichner
J E
JE
Department of Epidemiology, Graduate School of Public Health, University of Pittsburgh, Pa.
Kamboh
M I
MI
Cook
T
T
Ferrell
R E
RE
D001798:Blood Proteins / Q000235:genetics*; D015153:Blotting, Western; D014644:Genetic Variation; D006801:Humans; D007525:Isoelectric Focusing; D010641:Phenotype; D014156:Transcortin / Q000235:genetics*
D001798:Blood Proteins; D014156:Transcortin