title: [Application of dipeptidylpeptidase IV in formation of the peptide bond].
abstract: According to the increasing importance of enzyme catalysed peptide synthesis the capability of the protease dipeptidyl peptidase IV is shown to form the peptide bond. To our best knowledge, it's the first time that has been used successfully proline as one of the amino acids. The formation of the peptide bond is kinetically controlled. Free amino acids have been used as nucleophiles. The reaction media contain 50 vol.-% glycerol. A first approach to a kinetic treatment is shown.
authors.affiliation: Pädagogische Hochschule N.K. Krupskaja, Halle-Köthen, Martin-Luther-Universität Halle-Wittenberg, Biotechnikum.
authors.forename: P
authors.initials: P
authors.lastname: Heiduschka
authors.forename: J
authors.initials: J
authors.lastname: Dittrich
authors.forename: J
authors.initials: J
authors.lastname: Heins
authors.forename: K
authors.initials: K
authors.lastname: Neubert
authors.forename: A
authors.initials: A
authors.lastname: Barth
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D018819:Dipeptidyl Peptidase 4; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000032:analysis*; D005990:Glycerol / Q000032:analysis; D006868:Hydrolysis; D007700:Kinetics; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D010455:Peptides; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D018819:Dipeptidyl Peptidase 4; D005990:Glycerol
vernacular_title: Die Verwendung der Dipeptidylpeptidase IV zur Knüpfung von Peptidbindungen.