title: Whole blood aggregation using impedance and particle counter methods.
abstract: Platelet function in platelet-rich plasma may be evaluated using an optical or electrical impedance method. Platelet function in whole blood may be studied using an impedance or particle counter method. A comparison of these techniques in whole blood has not been performed. Twenty-four healthy subjects were studied using both techniques over a range of concentrations of collagen and adenosine diphosphate (ADP). Although broad similarities exist, discordant patterns occur in individual subjects. The particle counter method is capable of detecting small aggregates and thus is more sensitive. The impedance aggregometer detects only larger aggregate formation. These techniques are complementary rather than competitive and may have considerable potential in profiling platelet function.
authors.affiliation: Hemophilia Center of Western New York, Buffalo.
authors.forename: J D
authors.initials: JD
authors.lastname: Sweeney
authors.forename: J W
authors.initials: JW
authors.lastname: Labuzzetta
authors.forename: C E
authors.initials: CE
authors.lastname: Michielson
authors.forename: J E
authors.initials: JE
authors.lastname: Fitzpatrick
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D004553:Electric Conductivity / Q000502:physiology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010974:Platelet Aggregation / Q000502:physiology*; D010976:Platelet Count
chemical_list: D000244:Adenosine Diphosphate; D003094:Collagen