Developmental and hormonal regulation of alpha 2u-globulin gene transcription.
Hepatic alpha 2u-globulin protein and RNA levels are under developmental and complex multihormonal control. The present studies directly evaluate the degree to which this regulation is transcriptional. alpha 2u-Globulin transcription was determined by measuring nuclear runoff RNA in vitro, and tissue alpha 2u-globulin mRNA levels were measured by dot blot hybridization. These studies reveal that (i) in male rats the transcriptional rate of the alpha 2u-globulin genes increases during postnatal development; (ii) no alpha 2u-globulin transcription is detectable in hepatic nuclei derived from hypophysectomized rats; (iii) growth hormone and glucocorticoid are both absolutely required, and glucocorticoid can replace androgen for alpha 2u-globulin gene transcription in the livers of hypophysectomized male rats; and (iv) chronic treatment of mature male rats with estrogen results in a progressive decrease in the hepatic transcription of alpha 2u-globulin genes. In all instances changes in the transcriptional rate of alpha 2u-globulin genes paralleled the tissue level of alpha 2u-globulin RNA. Thus transcriptional control predominates in regulating hepatic alpha 2u-globulin RNA levels.
Kulkarni
A B
AB
Gubits
R M
RM
Feigelson
P
P
D000367:Age Factors; D000510:Alpha-Globulins / Q000096:biosynthesis / Q000235:genetics*; D000818:Animals; D004967:Estrogens / Q000494:pharmacology; D005260:Female; D005786:Gene Expression Regulation; D006728:Hormones / Q000494:pharmacology / Q000502:physiology*; D007016:Hypophysectomy; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D008297:Male; D009693:Nucleic Acid Hybridization; D012319:RNA Polymerase II / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012737:Sex Factors; D014158:Transcription, Genetic / Q000187:drug effects
D000510:Alpha-Globulins; D004967:Estrogens; D006728:Hormones; D012333:RNA, Messenger; C013803:alpha 2u globulin; D012319:RNA Polymerase II