title: In vitro activity of netilmicin alone and in combination with azlocillin, mezlocillin and imipenem against 149 coagulase-negative staphylococci.
abstract: Coagulase-negative staphylococci (CNS) have long been regarded as innocuous skin commensals with little pathogenic potential but they have recently become, under appropriate conditions, an important cause of infections. In fact, infections caused by CNS are an increasing problem especially, but not exclusively, in immuno-compromised patients. A total of 149 strains of CNS were identified from 47 patients admitted to the Haematology Department of Pescara Hospital from October 1986 to November 1987. The strains, isolated from different parts of the body and characterized by their methicillin susceptibility, were classified by API-Staph in 11 different groups. MICs and MBCs of netilmicin alone and combined with azlocillin, mezlocillin and imipenem were studied. For all combinations FIC and FBC indices were determined. The killing kinetics of the drugs mentioned above were also determined. Except for a few microorganisms (less than 5%), the associations showed a synergic or additive effect.
authors.affiliation: Institute of Experimental Medicine, Department of Microbiology, University G. D'Annunzio, Chieti, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Piccolomini
authors.forename: L
authors.initials: L
authors.lastname: Cellini
authors.forename: N
authors.initials: N
authors.lastname: Allocati
authors.forename: A
authors.initials: A
authors.lastname: Di Girolamo
authors.forename: M R
authors.initials: MR
authors.lastname: D'Eletto
authors.forename: D
authors.initials: D
authors.lastname: D'Antonio
authors.forename: G
authors.initials: G
authors.lastname: Ravagnan
mesh_terms: D001390:Azlocillin / Q000494:pharmacology*; D002490:Central Nervous System / Q000382:microbiology; D003030:Coagulase / Q000378:metabolism*; D004359:Drug Therapy, Combination / Q000494:pharmacology; D006801:Humans; D015378:Imipenem / Q000494:pharmacology*; D007700:Kinetics; D008802:Mezlocillin / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D009428:Netilmicin / Q000494:pharmacology*; D012015:Reference Standards; D013210:Staphylococcus / Q000187:drug effects* / Q000201:enzymology
chemical_list: D003030:Coagulase; D009428:Netilmicin; D015378:Imipenem; D001390:Azlocillin; D008802:Mezlocillin