[Immunocytochemical characterization of the DNA-polymerase alpha-positive colonic mucosal epithelial cells in patients with ulcerative colitis].
Colonic mucosal epithelial cells (EpC) in patients with ulcerative colitis (UC) have been shown to express HLA-DR antigen. In the present study, we observed the characteristics of HLA-DR-positive EpC using immunoperoxidase technique. In the control group, colonic EpC expressed HLA-ABC, but not HLA-DR. Only the EpC at the base of glands revealed positive for DNA-polymerase alpha (DNA-P). On the other hand, in actively inflamed mucosa of UC, about 80% of glands strongly expressed HLA-DR. Furthermore, most of EpC in the HLA-DR-positive glands showed positive nuclear stainings for DNA-P. This indicates that these EpC are not in the resting stage. It is strongly suggested that there are close relationships between the regeneration or proliferation of the EpC and class II MHC (HLA-DR) expression on the EpC in UC.
Matsumoto
T
T
Kitano
A
A
Nakamura
S
S
Oshitani
N
N
Obata
A
A
Hashimura
H
H
Hiki
M
M
Okawa
K
K
Nagura
H
H
Kobayashi
K
K
D000293:Adolescent; D000328:Adult; D003093:Colitis, Ulcerative / Q000201:enzymology* / Q000276:immunology; D003106:Colon / Q000201:enzymology* / Q000276:immunology; D004257:DNA Polymerase II / Q000032:analysis*; D004848:Epithelium / Q000201:enzymology / Q000276:immunology; D005260:Female; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000201:enzymology* / Q000276:immunology; D008297:Male; D008875:Middle Aged
D006684:HLA-DR Antigens; D004257:DNA Polymerase II