Stable high level expression of human thyroid peroxidase in cultured Chinese hamster ovary cells.
An expression plasmid containing both human thyroid peroxidase and mouse dihydrofolate reductase cDNAs was transfected into chinese hamster ovary cells. The stably transformed cells constitutively expressed immunoreactive thyroid peroxidase on the cell surface. These cells were further used to establish a subline producing a large amount of thyroid peroxidase by selecting clones resistant to methotrexate. The molecular weight of the expressed thyroid peroxidase was the same as purified human thyroid peroxidase. This expressed protein had peroxidase activity when determined by guaiacol oxidation. Furthermore, the expressed thyroid peroxidase was immunoreactive to sera of patients with autoimmune thyroid disease in which autoantibodies to thyroid peroxidase appeared.
Hata
J
J
Central Research Laboratory, Nippon Suisan Kaisha, Ltd., Tokyo, Japan.
Yamashita
S
S
Yagihashi
S
S
Kato
H
H
Kabeno
S
S
Hirai
K
K
Kuma
K
K
Kimura
S
S
Umeki
K
K
Kotani
T
T
D000818:Animals; D001323:Autoantibodies; D015139:Blotting, Southern; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D015870:Gene Expression; D006111:Graves Disease / Q000276:immunology; D006139:Guaiacol / Q000378:metabolism; D006801:Humans; D007074:Immunoglobulin G; D007453:Iodide Peroxidase / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D010053:Ovary; D010957:Plasmids; D013762:Tetrahydrofolate Dehydrogenase / Q000096:biosynthesis / Q000235:genetics; D013967:Thyroiditis, Autoimmune / Q000276:immunology; D014162:Transfection
D001323:Autoantibodies; D007074:Immunoglobulin G; D006139:Guaiacol; D007453:Iodide Peroxidase; D013762:Tetrahydrofolate Dehydrogenase