Efficient cell proliferation and predominant accumulation of epsilon-globin mRNA in human leukemic K562 cells which produce mostly Hb Gower 1.
Long-term cultures of K562(S) cells in 50-75 microM hemin allow the selection of 'hemin-resistant' K562 cells together with cells which proliferate efficiently while fully induced to express the human embryonic globin genes, as the hemoglobin Gower 1 (zeta 2 epsilon 2) is the predominant hemoglobin produced. Our experiments demonstrate that these K562 cells accumulate mostly epsilon-globin mRNA (epsilon-globin mRNA/gamma-globin mRNA = 2.9) suggesting that the control of hemoglobin expression is at a pretranslational level.
Gambari
R
R
Amelotti
F
F
Piva
R
R
D002455:Cell Division; D002478:Cells, Cultured; D005319:Fetal Hemoglobin / Q000096:biosynthesis*; D005786:Gene Expression Regulation / Q000187:drug effects; D005914:Globins / Q000096:biosynthesis* / Q000235:genetics; D006427:Hemin / Q000494:pharmacology; D006455:Hemoglobins, Abnormal / Q000096:biosynthesis*; D006801:Humans; D007938:Leukemia / Q000097:blood* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics
D006455:Hemoglobins, Abnormal; D012333:RNA, Messenger; C030446:hemoglobin Gower; D006427:Hemin; D005914:Globins; D005319:Fetal Hemoglobin