Molecular mechanisms of gossypol action on sperm motility.
1. Gossypol acetic acid inhibits collective motility of ejaculated ram spermatozoa. 2. Oxygen consumption was stimulated at low gossypol concentrations and inhibited as the concentrations are increased. 3. Gossypol inhibits respiration of permeabilized spermatozoa supported by durohydroquinome, which indicates a direct inhibition of mitochondrial electron transport chain. 4. The rapid reduction of mitochondrial dependent motility, high uncoupling effect and almost complete inhibition of mitochondrial calcium accumulation, indicate that gossypol inhibits motility in a mechanism by which mitochondrial uncoupling is involved.
Breitbart
H
H
Department of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel.
Mayevsky
A
A
Nass-Arden
L
L
D000818:Animals; D002118:Calcium / Q000378:metabolism; D006072:Gossypol / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D010101:Oxygen Consumption / Q000187:drug effects; D012756:Sheep; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors
D006072:Gossypol; D002118:Calcium