title: Isolation of Japanese encephalitis virus from mosquitoes collected in Karnataka state, India from 1985 to 1987.
abstract: Detection and isolation of Japanese encephalitis (JE) virus using mosquito inoculation and immunofluorescence techniques were attempted from female mosquitoes collected in JE endemic areas of Kolar and Mandya districts of Karnataka state, India, from 1985 to 1987. 65,388 mosquitoes consisting of 19 species in 1541 pools were processed. Of these, 18 pools showed the presence of JE virus antigen. JE virus was isolated from 9 pools, 3 of Culex gelidus, 2 of C. tritaeniorhynchus, and one each of C. quinquefasciatus, C. fuscocephala, C. vishnui and Anopheles peditaeniatus. Isolation of JE virus from C. gelidus, C. fuscocephala, C. quinquefasciatus and An. peditaeniatus is reported for the first time in India.
authors.affiliation: National Institute of Virology, Pune, India.
authors.forename: D T
authors.initials: DT
authors.lastname: Mourya
authors.forename: M A
authors.initials: MA
authors.lastname: Ilkal
authors.forename: A C
authors.initials: AC
authors.lastname: Mishra
authors.forename: P G
authors.initials: PG
authors.lastname: Jacob
authors.forename: U
authors.initials: U
authors.lastname: Pant
authors.forename: S
authors.initials: S
authors.lastname: Ramanujam
authors.forename: M S
authors.initials: MS
authors.lastname: Mavale
authors.forename: H R
authors.initials: HR
authors.lastname: Bhat
authors.forename: V
authors.initials: V
authors.lastname: Dhanda
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D009033:Culicidae / Q000382:microbiology*; D004664:Encephalitis Virus, Japanese / Q000276:immunology / Q000302:isolation & purification*; D005260:Female; D007194:India
chemical_list: D000956:Antigens, Viral