title: Effect of human chorionic gonadotropin and prostaglandin F2a on progesterone production by human luteal cells.
abstract: To determine and compare the direct effects of prostaglandin F2a (PGF2a) and human chorionic gonadotropin (hCG) on luteal cell progesterone production in vitro, 9 human corpora lutea obtained at tubal ligation were minced and treated with collagenase to disaggregate luteal cells. Dispersed luteal cells (80% viable) were incubated in air at 37 degrees C in a shaking water bath for 3 h and total progesterone in the media and cells was determined by radioimmunoassay. Optimum progesterone production was obtained using 25,000 or more cells per incubate and an incubation time of 2-4 h. hCG-stimulated progesterone production increased significantly with 0.01 IU to as high as 100 IU. In the early luteal phase (days 1-5 post ovulation or days 15-20 of the luteal phase), PGF2a (10-1000 ng) significantly inhibited progesterone production but significantly stimulated progesterone production in the mid-luteal phase (days 21-25). PGF2a had no effect on luteal cell progesterone production in the late luteal phase (days 26-30). This age-dependent direct effect of PGF2a on human luteal cell progesterone production in vitro indicates a role for PGF2a in the total intragonadal regulation of progesterone output, possibly through a paracrine or autocrine manner directed towards synchronizing luteal progesterone secretion and endometrial preparation for nidation.
authors.affiliation: Department of Obstetrics and Gynecology, University of Illinois College of Medicine, Chicago 60612.
authors.forename: F S
authors.initials: FS
authors.lastname: Khan-Dawood
authors.forename: J C
authors.initials: JC
authors.lastname: Huang
authors.forename: M Y
authors.initials: MY
authors.lastname: Dawood
mesh_terms: D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D003338:Corpus Luteum / Q000378:metabolism*; D015237:Dinoprost / Q000494:pharmacology*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008184:Luteal Cells / Q000378:metabolism*; D008183:Luteal Phase; D011374:Progesterone / Q000096:biosynthesis*
chemical_list: D006063:Chorionic Gonadotropin; D011374:Progesterone; D015237:Dinoprost