title: Immunological detection of arrestin, a phototransduction regulatory protein, in the cytosol of nucleated erythrocytes.
abstract: Cytosolic extracts of trout and turkey erythrocytes were tested for their immunoreactivity with polyclonal and monoclonal antibodies to retinal arrestin (S-antigen), a cytosolic protein of photoreceptor cells involved in the desensitization of rhodopsin. After adsorption or immunoaffinity chromatography of the extracts, these antibodies specifically recognized a protein having a molecular weight similar to that of retinal arrestin. Because the G-protein-mediated transduction systems, such as visual and beta-adrenergic systems, display a high degree of structural and functional homology, the presence of arrestin-like proteins in non-photosensitive cells suggests that these proteins are involved in the transduction of chemical signals, with a possible role in receptor desensitization.
authors.affiliation: INSERM U86, Institut Biomédical des Cordeliers, Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Mirshahi
authors.forename: F
authors.initials: F
authors.lastname: Borgese
authors.forename: A
authors.initials: A
authors.lastname: Razaghi
authors.forename: U
authors.initials: U
authors.lastname: Scheuring
authors.forename: F
authors.initials: F
authors.lastname: Garcia-Romeu
authors.forename: J P
authors.initials: JP
authors.lastname: Faure
authors.forename: R
authors.initials: R
authors.lastname: Motais
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis* / Q000302:isolation & purification; D019393:Arrestin; D002467:Cell Nucleus / Q000032:analysis; D002846:Chromatography, Affinity; D003600:Cytosol / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes / Q000032:analysis*; D005136:Eye Proteins / Q000032:analysis* / Q000302:isolation & purification; D007106:Immune Sera; D007118:Immunoassay; D008565:Membrane Proteins / Q000032:analysis*; D008970:Molecular Weight; D015398:Signal Transduction; D014337:Trout; D014422:Turkeys
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D019393:Arrestin; D005136:Eye Proteins; D007106:Immune Sera; D008565:Membrane Proteins