[Detection of intermediates of RNA reverse transcription for mobile dispersed MDG 1 and MDG 3 genes in Drosophila cells].
Poly(A)+RNA-DNA hybrids complementary to mobile dispersed genetic elements MDG 1 and MDG 3 have been isolated from Drosophila melanogaster culture cells. DNA and RNA in these complexes are represented by perfect hybrids. Three types of single-stranded DNA are detected in these hybrids: full-length DNA molecules of MDG containing one and two long terminal repeats (minus strand) and the long terminal repeat itself (plus strand). The results are consistent with the model of reverse transcriptional replication of MDG similar to that of retroviruses.
Il'in
Iu V
IuV
Arkhipova
I R
IR
Gorelova
T V
TV
Shuppe
N G
NG
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004270:DNA, Circular / Q000032:analysis / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D005111:Extrachromosomal Inheritance; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000032:analysis; D012313:RNA / Q000032:analysis / Q000235:genetics*; D012194:RNA-Directed DNA Polymerase / Q000235:genetics; D014158:Transcription, Genetic
D004251:DNA Transposable Elements; D004270:DNA, Circular; D011061:Poly A; D012313:RNA; D012194:RNA-Directed DNA Polymerase
Obnaruzhenie intermediatov obratnoĭ transkriptsii RNK mobil'nykh dispergirovannykh genov MDG 1 i MDG 3 v kletkakh drozofily.