The 5' noncoding region of the human leukemia-associated oncogene BCR/ABL is a potent inhibitor of in vitro translation.
The mRNA encoding the chimeric BCR/ABL oncogene, which is transcribed from the Philadelphia chromosome in human chronic myelogenous leukemia, has a 5' noncoding sequence greater than 500 bases in length which is highly GC rich and contains a short open reading frame. This untranslated sequence has a dramatic inhibitory effect upon translational efficiency in vitro. However, when BCR/ABL message is expressed in certain cell types such as the NIH 3T3 cell line, the 5' noncoding region has little inhibitory effect on translational efficiency.
Muller
A J
AJ
Molecular-Biology Institute, University of California, Los Angeles 90024.
Witte
O N
ON
D001482:Base Composition; D001483:Base Sequence; D002678:Chimera; D006801:Humans; D007438:Introns; D015466:Leukemia, Myeloid, Chronic-Phase / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009857:Oncogenes; D010677:Philadelphia Chromosome; D014176:Protein Biosynthesis; D014158:Transcription, Genetic