Time-resolved fluoroimmunoassay of aflatoxins.
Preparation of purified and Eu-labeled antibodies specific for aflatoxins is described. Their use is illustrated by a solid-phase competitive time-resolved fluoroimmunoassay, results of which were correlated with those of an enzyme-linked immunosorbent assay based on use of the unmodified antibody to aflatoxin. This procedure is discussed as a quick, sensitive, and reliable immunoassay for use in mycotoxin screening in foodstuffs and body fluids.
Degan
P
P
National Institute for Research on Cancer, Genova, Italy.
Montagnoli
G
G
Wild
C P
CP
D000348:Aflatoxins / Q000032:analysis* / Q000276:immunology; D000818:Animals; D000918:Antibody Specificity; D001826:Body Fluids / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005063:Europium; D015200:Fluoroimmunoassay; D005504:Food Analysis; D006801:Humans; D009181:Mycoses / Q000175:diagnosis; D004369:Pentetic Acid
D000348:Aflatoxins; D005063:Europium; D004369:Pentetic Acid