title: Simple and rapid identification of phosphorylated peptides from bovine brain myelin basic protein by reversed-phase high-performance liquid chromatography.
abstract: The phosphorylation sites of the myelin basic protein from bovine brain were determined after phosphorylation with a cyclic 3':5'-phosphate-dependent protein kinase from the same source. Three phosphorylated peptides were selectively and rapidly separated, before and after dephosphorylation, by reversed-phase high-performance liquid chromatography on a styrene 250 column under alkaline conditions. Partial sequencing of the peptides by automated Edman degradation revealed that the serine-115 residue located in the main encephalitogenic determinant of the protein was a phosphorylation site, in addition to the two phosphorylation sites established (threonine-34 and serine-55).
authors.forename: S
authors.initials: S
authors.lastname: Shoji
authors.forename: J
authors.initials: J
authors.lastname: Ohnishi
authors.forename: T
authors.initials: T
authors.lastname: Funakoshi
authors.forename: Y
authors.initials: Y
authors.lastname: Kubota
authors.forename: K
authors.initials: K
authors.lastname: Fukunaga
authors.forename: E
authors.initials: E
authors.lastname: Miyamoto
authors.forename: H
authors.initials: H
authors.lastname: Ueki
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006868:Hydrolysis; D004676:Myelin Basic Protein / Q000032:analysis*; D010455:Peptides / Q000032:analysis*; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D014357:Trypsin
chemical_list: D000596:Amino Acids; D004676:Myelin Basic Protein; D010455:Peptides; D011494:Protein Kinases; D014357:Trypsin