Quantitation of platelet loss with indium-111 labeled platelets in a hollow-fiber membrane oxygenator and arterial filter during extracorporeal circulation in a pig model.
Platelet consumption in a hollow-fiber membrane oxygenator (HFMO) and arterial filter (AF) during cardiopulmonary bypass (CPB) was quantified in five pigs using Indium-111 labeled autologous platelets. Platelet labeling was performed 20-24 hours before CPB. After general endotracheal anesthesia, the pigs were systemically heparinized and were placed on CPB via a median sternotomy. After 3 hours of CPB, radioactivity was quantified with a gamma camera and an ionization chamber. The percent of injected dose (mean +/- SD) was 0.79 +/- 0.45 in the HFMO, 2.52 +/- 0.93 in AF, 4.3 +/- 1.2 in blood loss during CPB. Platelet consumption in HFMO during CPB was lower than in bubble oxygenators (19%) or silicone membrane oxygenators (12%) as observed in previous studies.
Palatianos
G M
GM
Divisions of Thoracic and Cardiovascular Surgery, University of Miami School of Medicine, FL 33101.
Dewanjee
M K
MK
Robinson
R P
RP
Novak
S
S
Dewanjee
P K
PK
Kapadvanjwala
M
M
Hsu
L C
LC
Sfakianakis
G N
GN
Kaiser
G A
GA
D000818:Animals; D001792:Blood Platelets; D004867:Equipment Design; D005260:Female; D006440:Hemofiltration / Q000295:instrumentation*; D007205:Indium Radioisotopes; D009942:Organometallic Compounds; D010107:Oxygenators, Membrane; D010976:Platelet Count; D011877:Radionuclide Imaging; D013552:Swine; D013921:Thrombocytopenia / Q000000981:diagnostic imaging*; D014334:Tropolone / Q000031:analogs & derivatives
D007205:Indium Radioisotopes; D009942:Organometallic Compounds; C043895:indium tris(tropolonate); D014334:Tropolone