Ca-tolerant guinea-pig ventricular myocytes as isolated by pronase in the presence of 250 microM free calcium.
A new method to isolate adult cardiocytes with pronase in the presence of 250 microM free calcium was developed. Ultrastructural and electrophysiological properties of these cells were investigated. It is shown that by this method normal calcium-tolerant cells can be obtained.
Bendukidze
Z
Z
Isenberg
G
G
Klöckner
U
U
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002463:Cell Membrane Permeability; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D003470:Culture Media; D004562:Electrocardiography; D006168:Guinea Pigs; D006352:Heart Ventricles / Q000166:cytology; D007473:Ion Channels / Q000378:metabolism; D008854:Microscopy, Electron; D009206:Myocardium / Q000166:cytology*; D011402:Pronase
D003470:Culture Media; D007473:Ion Channels; D011402:Pronase; D002118:Calcium