title: Induction of interleukin-1 production in patients undergoing cardiopulmonary bypass.
abstract: Systemic reactions resembling inflammation occur in patients undergoing cardiopulmonary bypass. We now report that interleukin-1, an endogenous pyrogen and a key mediator of inflammation, is transiently and consistently generated in vivo by circulating monocytes within hours after cardiopulmonary bypass. Interleukin-1 production was assessed by measuring interleukin-1 functional activity and interleukin-1 beta antigen concentration in cell lysates from monocytes of patients during and after bypass. There was no increase in intracellular interleukin-1 activity during bypass and within the first hours after bypass, possibly because of a suppressive effect of hypothermia on interleukin-1 production, as documented in vitro. Maximal generation of interleukin-1 was observed 24 hours after extracorporeal circulation, concomitantly with the occurrence of a peak in body temperature. The amount of interleukin-1 generated at that time was linearly correlated with the increase in patients' body temperature. The peak in interleukin-1 production followed by 20 hours the peak in complement activation as assessed by determining C3a desArg and C5a desArg concentrations in patients' plasma. These results indicate that interleukin-1 may be involved in the pathogenesis of adverse systemic reactions associated with cardiopulmonary bypass.
authors.affiliation: INSERM U28, Université Paris VI, France.
authors.forename: N
authors.initials: N
authors.lastname: Haeffner-Cavaillon
authors.forename: N
authors.initials: N
authors.lastname: Roussellier
authors.forename: O
authors.initials: O
authors.lastname: Ponzio
authors.forename: M P
authors.initials: MP
authors.lastname: Carreno
authors.forename: M
authors.initials: M
authors.lastname: Laude
authors.forename: A
authors.initials: A
authors.lastname: Carpentier
authors.forename: M D
authors.initials: MD
authors.lastname: Kazatchkine
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001831:Body Temperature; D002315:Cardiopulmonary Bypass / Q000009:adverse effects; D003167:Complement Activation; D006801:Humans; D007036:Hypothermia, Induced; D007375:Interleukin-1 / Q000096:biosynthesis*; D007700:Kinetics; D007958:Leukocyte Count; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D013997:Time Factors
chemical_list: D007375:Interleukin-1