title: Diffusion barrier properties of the perineurium: an in vivo ionic lanthanum tracer study.
abstract: While the perineurium as a diffusion barrier has been extensively investigated by light and electron microscopy, such studies have been largely restricted to the use of protein tracers. In the present study the permeability of the perineurium to a physiologically more relevant ionic tracer has been assessed. In vivo the rat sural or tibial nerve was either microinjected with lanthanum nitrate solution for endoneurial application or bathed in the lanthanum solution for epineurial application. The findings generally demonstrated an effective barrier to the tracer which failed to penetrate the inner layers of the perineurium. Only at the highest lanthanum concentration and longest time intervals employed did trace quantities occasionally penetrate the barrier and then only in the presence of some cytopathological changes to the outermost perineurial cells. The usefulness of the microinjection method was limited by the slight but unavoidable trauma to the perineurium. The findings are related to those of other studies which have used electron dense tracers, also to studies using physiological including electrophysiological techniques and morphological including freeze-fracture methods.
authors.affiliation: Department of Anatomy and Cell Biology, Charing Cross and Westminster Medical School, London, England.
authors.forename: M N
authors.initials: MN
authors.lastname: Ghabriel
authors.forename: K H
authors.initials: KH
authors.lastname: Jennings
authors.forename: G
authors.initials: G
authors.lastname: Allt
mesh_terms: D000818:Animals; D004058:Diffusion; D007811:Lanthanum / Q000378:metabolism*; D008297:Male; D008845:Microinjections; D008854:Microscopy, Electron; D009474:Neurons / Q000502:physiology*; D010539:Permeability; D051381:Rats; D011919:Rats, Inbred Strains; D013497:Sural Nerve / Q000502:physiology / Q000648:ultrastructure; D013979:Tibial Nerve / Q000502:physiology
chemical_list: D007811:Lanthanum