title: Cell receptors for the mammalian reovirus. IV. Reovirus-specific cytolytic T cell lines that have idiotypic receptors recognize anti-idiotypic B cell hybridomas.
abstract: Cytotoxic T lymphocyte (Tc) cell lines specific for reovirus type 3 lysed an uninfected B cell hybridoma line, 87.92.6, that expresses and secretes an anti-idiotypic antibody that reacts with an anti-viral hemagglutinin monoclonal antibody, 9BG5. Monoclonal anti-idiotype 87.92.6 was shown by fluorescence analysis to specifically bind to reovirus Tc and to block reovirus-specific Tc from killing reovirus-infected target cells or the 87.92.6 hybridoma. An anti-LFA-1 monoclonal antibody, M17, interfered with Tc-mediated lysis of reovirus-infected targets and the 87.92.6 cells, indicating the similarity of cellular interactions mediated by LFA-1 structures when Tc bind to virally infected targets or 87.92.6 targets. Together with studies in which anti-H2 or monoclonal idiotypic antibodies were found to interfere with reovirus-specific Tc recognition of virally infected or 87.92.6 targets, these experiments indicate that some reovirus-specific Tc have conformations in their receptor that can be recognized by anti-idiotype.
authors.forename: A H
authors.initials: AH
authors.lastname: Sharpe
authors.forename: G N
authors.initials: GN
authors.lastname: Gaulton
authors.forename: H C
authors.initials: HC
authors.lastname: Ertl
authors.forename: R W
authors.initials: RW
authors.lastname: Finberg
authors.forename: K K
authors.initials: KK
authors.lastname: McDade
authors.forename: B N
authors.initials: BN
authors.lastname: Fields
authors.forename: M I
authors.initials: MI
authors.lastname: Greene
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology; D000954:Antigens, Surface / Q000032:analysis; D000956:Antigens, Viral / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D001667:Binding, Competitive; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D006825:Hybridomas / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000276:immunology / Q000378:metabolism*; D012089:Mammalian orthoreovirus 3 / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D011948:Receptors, Antigen, T-Cell / Q000032:analysis*; D011991:Receptors, Virus / Q000032:analysis*; D012087:Reoviridae / Q000378:metabolism*; D012088:Reoviridae Infections / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000956:Antigens, Viral; D000939:Epitopes; D007130:Immunoglobulin Idiotypes; D011948:Receptors, Antigen, T-Cell; D011991:Receptors, Virus