title: Association of the glucocorticoid hormone receptor with ribonucleic acid.
abstract: The hypothesis that the glucocorticoid hormone receptor interacts with RNA has been tested in cultured rat hepatoma cells. The receptor was covalently labeled with radioactive dexamethasone mesylate, and putative RNA-receptor complexes were stabilized by either cell-free crosslinking using formaldehyde or irradiation of intact cells. After chemical cross-linking in vitro, the receptor displayed the buoyant density of a ribonucleoprotein in CsCl gradients. After photochemical crosslinking in cells labeled with radioactive uridine, the receptor analysed by polyacrylamide gel electrophoresis was carrying labeled ribonucleotides.
authors.forename: I V
authors.initials: IV
authors.lastname: Economidis
authors.forename: G G
authors.initials: GG
authors.lastname: Rousseau
mesh_terms: D000818:Animals; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D003907:Dexamethasone / Q000031:analogs & derivatives / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005557:Formaldehyde / Q000494:pharmacology; D008114:Liver Neoplasms, Experimental / Q000378:metabolism; D010777:Photochemistry; D012313:RNA / Q000378:metabolism*; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*; D014529:Uridine / Q000378:metabolism
chemical_list: D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; D005557:Formaldehyde; D012313:RNA; D003907:Dexamethasone; C031710:dexamethasone 21-methanesulfonate; D014529:Uridine