title: Protein clearance from the air spaces and lungs of unanesthetized sheep over 144 h.
abstract: We studied the rate, the routes, and the mechanisms for protein clearance from the air spaces and lungs of 20 unanesthetized sheep over 144 h. We instilled 100 ml of autologous serum labeled with 125I-albumin into one lung. At the end of 24, 48, 96, or 144 h, the lungs were removed and the residual native protein and 125I-albumin in the air spaces were determined by bronchoalveolar lavage. Also the fraction of the instilled 125I-albumin remaining in the rest of the lung was measured in the lung homogenate. Clearance of the 125I-albumin from the lung into the plasma, lymph, thyroid, urine, and feces was also determined. The removal of both the 125I-albumin and the native protein from the air spaces was slow, following a monoexponential decline. The removal rate of the 125I-albumin from the air spaces was slightly but significantly faster (1.6%/h) than the clearance rate of the native protein (0.9%/h). Clearance of the 125I-albumin from the lung also followed a slow monoexponential decline at a rate of 1.4%/h. At all time periods, 75% of the 125I-albumin remaining in the lung was located in the air spaces, thus indicating that the pulmonary epithelium is the principal barrier to protein clearance from the normal lung. Macrophages appeared to play a minor role in alveolar protein clearance because the quantity of 125I-albumin present in the phagocytic cells in the air spaces was less than 1% of the instilled 125I-albumin at all time periods. However, macrophages may play some role in protein clearance after 48 h because we visualized phagolysosomes in macrophages, and there was an increase in free iodine in lung lavage, urine, thyroid, and feces after 48 h. However, gel electrophoretic studies showed that most of the 125I-albumin was cleared from the lung as an intact molecule, although only 24.7 +/- 4.7% of the 125I-albumin was cleared by the lymphatics.
authors.affiliation: Department of Medicine, University of California, San Francisco 94143.
authors.forename: Y
authors.initials: Y
authors.lastname: Berthiaume
authors.forename: K H
authors.initials: KH
authors.lastname: Albertine
authors.forename: M
authors.initials: M
authors.lastname: Grady
authors.forename: G
authors.initials: G
authors.lastname: Fick
authors.forename: M A
authors.initials: MA
authors.lastname: Matthay
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D001992:Bronchoalveolar Lavage Fluid / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism / Q000648:ultrastructure; D005243:Feces / Q000032:analysis; D005260:Female; D008168:Lung / Q000378:metabolism* / Q000648:ultrastructure; D008196:Lymph / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism / Q000648:ultrastructure; D011650:Pulmonary Alveoli / Q000378:metabolism* / Q000648:ultrastructure; D012711:Serum Albumin, Radio-Iodinated / Q000378:metabolism; D012756:Sheep; D013961:Thyroid Gland / Q000378:metabolism; D013997:Time Factors
chemical_list: D001798:Blood Proteins; D012711:Serum Albumin, Radio-Iodinated