title: Polymethylmethacrylate-induced release of bone-resorbing factors.
abstract: A pseudomembranous structure that has the histological characteristics of a foreign-body-like reaction invariably develops at the bone-cement interface in the proximity of resorption of bone around aseptically loosened cemented prostheses. This study was an attempt to implicate polymethylmethacrylate in this resorptive process. Unfractionated peripheral-blood mononuclear cells (consisting of lymphocytes and monocytes) and surface-adherent cells (monocyte-enriched) were prepared from control subjects who did and did not have clinical evidence of osteoarthrosis and from patients who had osteoarthrosis and were having a revision for failure of a cemented hip or knee implant. Cells were cultured for varying periods in the presence and absence of nonpolymerized methacrylate (one to two-micrometer spherules), pulverized polymerized material, or culture chambers that were pre-coated with polymerized cement. Conditioned media that were derived from both methacrylate-stimulated cell populations were shown to contain specific bone-resorbing mediators (interleukin-1, tumor necrosis factor, or prostaglandin E2) and to directly affect bone resorption in 45Ca-labeled murine limb-bone assays.
authors.affiliation: Department of Internal Medicine, University of Cincinnati College of Medicine, Ohio 45267.
authors.forename: J H
authors.initials: JH
authors.lastname: Herman
authors.forename: W G
authors.initials: WG
authors.lastname: Sowder
authors.forename: D
authors.initials: D
authors.lastname: Anderson
authors.forename: A M
authors.initials: AM
authors.lastname: Appel
authors.forename: C N
authors.initials: CN
authors.lastname: Hopson
mesh_terms: D000818:Animals; D001685:Biological Factors / Q000378:metabolism*; D002132:Calcium Radioisotopes; D002478:Cells, Cultured; D002852:Chromatography, Ion Exchange; D016207:Cytokines; D015232:Dinoprostone / Q000032:analysis; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism*; D008768:Methylmethacrylates / Q000494:pharmacology*; D051379:Mice; D008813:Mice, Inbred ICR; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: D001685:Biological Factors; D002132:Calcium Radioisotopes; D016207:Cytokines; D007375:Interleukin-1; D008768:Methylmethacrylates; D014409:Tumor Necrosis Factor-alpha; C025940:bone resorption factor; D015232:Dinoprostone