The G0 in equilibrium G1 transitions of human lymphocytes as monitored by quantitative 14C-uridine autoradiography and high-resolution image analysis.
Human lymphocytes, transferred in culture into medium conditioned with phytohemagglutinin, were studied with the combined use of quantitative 14C-uridine autoradiography and high-resolution image analysis. During the first 24 h before the onset of DNA synthesis it was possible to study pure G0 and G1 populations. The return of the proliferating cells into the G0 state was clearly monitored in an RNA synthesis rate/DNA content plot during 1-week growth. On days 3-6, the proliferation decreased progressively, and more cells accumulated in the quiescent G0 state. Geometric, densitometric, and textural parameters evaluated from the Feulgen-stained nuclei of the same cells indicated that the decondensation/condensation processes of the nuclear chromatin during the G0 in equilibrium G1 transitions paralleled the RNA synthesis activity. The results help to associate descriptors of nuclear morphology, as derived from image analysis, with functional parameters during activation and return to quiescence of human lymphocytes.
Giaretti
W
W
Abmayr
W
W
Dörmer
P
P
Santi
L
L
D001345:Autoradiography; D002478:Cells, Cultured; D004396:Coloring Agents; D004247:DNA / Q000378:metabolism; D006801:Humans; D007399:Interphase; D007700:Kinetics; D008214:Lymphocytes / Q000166:cytology* / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis; D012394:Rosaniline Dyes; D014529:Uridine / Q000378:metabolism
D004396:Coloring Agents; C007722:Feulgen stain; D012394:Rosaniline Dyes; D012313:RNA; D004247:DNA; D014529:Uridine