Effect of cold stress on cholinergic receptors in the rat adrenal gland.
The effects of cold exposure on cholinergic binding sites in the rat adrenal gland were assessed by examining the binding of [125I]alpha-bungarotoxin (BTX), a nicotonic receptor antagonist and [3H]quinuclidinyl benzilate (QNB), a muscarinic receptor antagonist, to adrenal tissue homogenates. Cold exposure resulted in significant alterations in both nicotinic and muscarinic binding. Exposure to cold for 4 and 7 days resulted in a significant decrease in QNB binding. Scatchard analysis indicates that this alteration is due to a decrease in binding sites (Bmax) rather than a change in ligand affinity (Kd). In contrast, chronic cold exposure produced a significant increase in BTX binding sites. These results indicate that adrenal cholinergic receptors are altered in reciprocal fashion by chronic cold exposure, and that this change may represent a key event in the sympathoadrenal system's adaptive response to chronic cold stress.
Miner
L L
LL
Western Psychiatric Institute and Clinic, University of Pittsburgh, PA 15213.
Baruchin
A
A
Kaplan
B B
BB
D000311:Adrenal Glands / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D002038:Bungarotoxins / Q000378:metabolism; D003080:Cold Temperature; D008297:Male; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011950:Receptors, Cholinergic / Q000378:metabolism* / Q000502:physiology; D013312:Stress, Physiological / Q000378:metabolism*
D002038:Bungarotoxins; D011950:Receptors, Cholinergic; D011813:Quinuclidinyl Benzilate