Laser fluorometric detection of porphyrin methyl esters for high-performance thin-layer chromatography.
A new detection method is presented for the determination of porphyrins present in biological materials. Separation is accomplished by high-performance thin-layer chromatography after esterification of individual carboxylic acid porphyrins. Detection is achieved by utilizing one of the visible lines of an argon-ion laser for fluorometric excitation. Good selectivity and detectability are demonstrated for the determination of porphyrin profiles in human urine. The detection limits for uro-, heptacarboxy-, hexacarboxy-, pentacarboxy-, copro-, and mesoporphyrin methyl esters are in the 18-35 pg range.
Huie
C W
CW
Williams
W R
WR
D002264:Carboxylic Acids / Q000652:urine*; D002855:Chromatography, Thin Layer; D004952:Esters / Q000652:urine*; D006801:Humans; D007834:Lasers; D011166:Porphyrins / Q000652:urine*; D013050:Spectrometry, Fluorescence
D002264:Carboxylic Acids; D004952:Esters; D011166:Porphyrins