[The use of the potentials of the molecular hybridization of nucleic acids as a method for the laboratory diagnosis of influenza in research on vaccinal infection].
The data obtained as the result of the complex examination of volunteers immunized with live influenza vaccine, type A (H3N2), showed that the determination of the RNA-containing structures by the method of the molecular hybridization of nucleic acids (MHNA) was highly sensitive and reliable. This method proved to be more sensitive than common laboratory diagnostic tests (the isolation of the virus in chick embryos, the analysis of seroconversion, the antibody fluorescence test) and was not inferior to the complex of clinical tests based on the analysis of microsymptoms. The reliability of the positive (according to the data obtained by MHNA) results was very high: not less than 85% of them were confirmed by other tests.
Pliuskin
A Z
AZ
Nolandt
O V
OV
Briantseva
E A
EA
Kubar'
O I
OI
Firsov
S L
SL
D000281:Administration, Intranasal; D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000032:analysis; D001483:Base Sequence; D015342:DNA Probes; D004274:DNA, Recombinant; D005069:Evaluation Studies as Topic; D006801:Humans; D009980:Influenza A virus / Q000235:genetics* / Q000276:immunology; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000276:immunology*; D007251:Influenza, Human / Q000175:diagnosis* / Q000517:prevention & control; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012367:RNA, Viral / Q000235:genetics*
D000914:Antibodies, Viral; D015342:DNA Probes; D004274:DNA, Recombinant; D007252:Influenza Vaccines; D012367:RNA, Viral
Ispol'zovanie vozmozhnosteĭ molekuliarnoĭ gibridizatsii nukleinovykh kislot kak metoda laboratornoĭ diagnostiki grippa pri issledovanii vaktsinal'noĭ infektsii.