title: Identification of a voltage-dependent anion channel in the apical membrane of a Cl(-)-secretory epithelium (MDCK).
abstract: An anion-selective channel of large unit conductance is present in apical membranes of a secretory epithelial cell. For cell-attached patch-clamp configuration on cultured MDCK cells channels with a conductance of about 460 pS are observed at 37 degrees C. The channel becomes spontaneously activated only in rare cases. In inside-out membrane patches the probability of observing this type of channel increased significantly suggesting that the channel is controlled by an up to now unknown mechanism. The channel shows a voltage-dependent burst kinetic.
authors.forename: H A
authors.initials: HA
authors.lastname: Kolb
authors.forename: C D
authors.initials: CD
authors.lastname: Brown
authors.forename: H
authors.initials: H
authors.lastname: Murer
mesh_terms: D000838:Anions / Q000378:metabolism* / Q000502:physiology; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D002712:Chlorides / Q000378:metabolism*; D004594:Electrophysiology; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D007473:Ion Channels / Q000502:physiology*; D007668:Kidney / Q000166:cytology / Q000378:metabolism*
chemical_list: D000838:Anions; D002712:Chlorides; D007473:Ion Channels