Arginine-vasopressin binding to isolated hippocampal microvessels of rats with different endogenous concentrations of the neuropeptide.
The binding of [125I]arginine-8-vasopressin (AVP) to hippocampal microvessels isolated from brains of normal Wistar rats, animals after water deprivation and heterozygous as well as homozygous diabetes-insipidus rats (Brattleboro strain) were measured. Data from binding experiments from the microvessels of the different groups of animals in each case revealed a single class of high affinity binding sites. However, the binding parameters between the different groups of rats were different. The affinity constants differs by a factor of 5.1, ranging from KD = 1.18 nmol X L-1 (animals after water deprivation) to KD = 6.05 nmol X L-1 (homozygous Brattleboro rats). The binding capacity, differing by a factor of 3.5, ranged from Bmax = 245 fmol X mg-1 to Bmax = 865 fmol X mg-1.
Kretzschmar
R
R
Dept. of Cell Biology and Regulation, Karl-Marx-University, Leipzig, GDR.
Ermisch
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A
D000818:Animals; D001127:Arginine Vasopressin / Q000378:metabolism*; D003919:Diabetes Insipidus / Q000378:metabolism; D006624:Hippocampus / Q000098:blood supply / Q000378:metabolism*; D066298:In Vitro Techniques; D008833:Microcirculation / Q000378:metabolism; D011869:Radioligand Assay; D051381:Rats; D011910:Rats, Brattleboro; D011919:Rats, Inbred Strains; D011945:Receptors, Angiotensin / Q000378:metabolism*; D017483:Receptors, Vasopressin; D014868:Water Deprivation / Q000502:physiology
D011945:Receptors, Angiotensin; D017483:Receptors, Vasopressin; D001127:Arginine Vasopressin