18-Hydroxylase activity in the Y1 adrenal cell line.
18-Hydroxylase activity, reported here for the first time in the mouse adrenal tumor cell line (Y1), was expressed in the metabolism of 11-deoxycorticosterone (DOC) and corticosterone (B). Detected after 24 h of incubation, it was more evident after 48 h and produced mostly 18-hydroxy-20 alpha-DHB from these exogenous substrates. However, 18-hydroxylation was quantitatively less significant than the metabolism of 20 alpha-reduction and 11 beta-hydroxylation (of DOC). The latter is also the predominant metabolism of progesterone in this cell line, during the conversion of cholesterol from the serum-supplemented culture media. The cytochrome P-450 11 beta activity of the Y1 cells is similar to that of the mouse in vivo which catalyzes the production of an 11 beta 18-dihydroxylated metabolite as the principal 18-hydroxylated steroid. It is different from that of other species, such as the rat and the bovine, both in terms of the ratio of 11 beta- to 18-hydroxylated metabolites and of the structure of these metabolites.
Ramirez
L C
LC
Laboratoire de Biochimie des Interactions Cellulaires, Faculté, de Sciences, Université de Bourgogne, Dijon, France.
Es-souni
M
M
Bournot
P
P
D000302:Adrenal Cortex / Q000201:enzymology*; D000818:Animals; D003345:Corticosterone / Q000378:metabolism; D019405:Cytochrome P-450 CYP11B2; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D003900:Desoxycorticosterone / Q000378:metabolism; D051379:Mice; D013250:Steroid Hydroxylases / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D003900:Desoxycorticosterone; D003577:Cytochrome P-450 Enzyme System; D013250:Steroid Hydroxylases; D019405:Cytochrome P-450 CYP11B2; D003345:Corticosterone