Downregulation of HLA class I antigens in HIV-1-infected cells.
By means of indirect immunofluorescence analysis we investigated the effect of HIV-1 infection on HLA class I surface antigens. We report here that in CD4+ HeLa cells, in H9 cells, and in peripheral T lymphocytes HLA class I antigens are downregulated following infection with HIV-1. The downregulation is effected at a posttranscriptional level since the amounts of HLA class I specific mRNA are similar in infected and uninfected cells. This phenomenon is not only correlated with the state of infection, that is, the presence of P24 of HIV-1 in the cells, but also depends on the time of infection. Upon HLA class I downregulation by HIV infection, the specific lysis of peripheral blood cells by allogeneic CTL is reduced.
Kerkau
T
T
Institut für Virologie und Immunobiologie der Universität, Würzburg, FRG.
Schmitt-Landgraf
R
R
Schimpl
A
A
Wecker
E
E
D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000382:microbiology; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D015683:Gene Products, gag / Q000378:metabolism; D016655:HIV Core Protein p24; D015497:HIV-1 / Q000276:immunology* / Q000378:metabolism; D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D006801:Humans; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000382:microbiology; D014758:Viral Core Proteins / Q000378:metabolism
D015683:Gene Products, gag; D016655:HIV Core Protein p24; D015395:Histocompatibility Antigens Class I; D014758:Viral Core Proteins