Routine measurement of plasma catecholamines in clinical pharmacology by high-performance liquid chromatography with electrochemical detection.
A method for the simultaneous determination of epinephrine, norepinephrine and dopamine in human plasma is described, which combines the advantages of liquid-liquid extraction sample preparation, high-performance liquid chromatography on weak cation-exchange stationary phases and dual-electrode coulometric detection. The limits of quantification are less than 5 pg/ml (at a signal-to-noise ratio greater than 5) for each analyte. The influence of various experimental parameters (e.g., composition of the mobile phase, pretreatment of the assay buffer, components of the re-extraction system) on the performance of the assay is reported in detail. A number of applications are presented, which demonstrate the quality of the data obtained in terms of sensitivity, reproducibility and significance.
Meineke
I
I
SK&F Institute for Applied Clinical Pharmacology, Göttingen, F.R.G.
Stüwe
E
E
Henne
E M
EM
Rusteberg
G
G
Brendel
E
E
De Mey
C
C
D002395:Catecholamines / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D006801:Humans
D002395:Catecholamines