A monoclonal antibody identifies a glycoprotein complex involved in cell-substratum adhesion.
The monoclonal antibody CSAT has been reported to perturb the adhesion of chick embryo cells to their substratum (Neff et al. [19]). Evidence is presented here that the antigen recognized by this monoclonal antibody is comprised of three membrane glycoproteins. The antigen is released from cells with non-ionic detergent and purified by monoclonal antibody affinity chromatography. When analysed by SDS-PAGE under non-reducing conditions, the antigen resolves into three components of apparent molecular weights 160 000 (band 1), 135000 (band 2), and 110 000 (band 3). Following reduction of each component, bands 1 and 2 migrate at slightly lower apparent molecular weights, while band 3 migrates at a higher apparent molecular weight, suggesting that band 3 has an internal disulfide bond. All three bands differ from one another as determined by peptide mapping and by immunologic cross-reactivity. It is postulated that the three glycoproteins function as a complex that plays a central role in cell-substratum adhesion.
Knudsen
K A
KA
Horwitz
A F
AF
Buck
C A
CA
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000911:Antibodies, Monoclonal; D001666:Binding Sites, Antibody; D002448:Cell Adhesion; D055598:Chemical Phenomena; D002621:Chemistry; D002642:Chick Embryo; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology; D005347:Fibroblasts; D006023:Glycoproteins / Q000032:analysis* / Q000502:physiology; D006026:Glycoside Hydrolases; D008565:Membrane Proteins / Q000032:analysis* / Q000502:physiology; D008970:Molecular Weight; D009132:Muscles / Q000166:cytology; D010446:Peptide Fragments / Q000032:analysis
D000596:Amino Acids; D000911:Antibodies, Monoclonal; D000939:Epitopes; D006023:Glycoproteins; D008565:Membrane Proteins; D010446:Peptide Fragments; D003101:Collodion; D006026:Glycoside Hydrolases