The transport of L-arginine in Chinese hamster ovary cells.
The transport of L-arginine has been characterized in Chinese hamster ovary cells (CHO). In the absence of Na+ the influx of the amino acid decreased. Both in the presence and in the absence of Na+ L-arginine influx was trans-stimulated and cis-inhibited by cationic amino acids. The amino acid entered CHO cells through an apparently non saturable mechanism and a single saturable agency whose Km increased in the absence of Na+. These results indicate that the agency devoted to transport cationic amino acids in CHO cells resembles system y+, the Na+-independent route that transports cationic amino acids in a number of mammalian models, although its activity is lowered by the replacement of extracellular sodium.
Rotoli
B M
BM
Istituto di Patologia Generale, Università di Parma, Italy.
Bussolati
O
O
Dall'Asta
V
V
Gazzola
G C
GC
D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D001120:Arginine / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D007700:Kinetics; D010053:Ovary; D012964:Sodium / Q000494:pharmacology
D000596:Amino Acids; D001120:Arginine; D012964:Sodium