title: Inhibitory effect of glycosides like saponin from soybean on the infectivity of HIV in vitro.
abstract: Soybean saponins isolated from soybean seeds were investigated for their antiviral activity on HIV in vitro, using an HTLV-I-carrying cell line, MT-4. Saponin B1 completely inhibited HIV-induced cytopathic effects and virus-specific antigen expression 6 days after infection at concentrations greater than 0.5 mg/ml. Saponin B2 also inhibited HIV infection, although less potently. Both saponin B1 and B2 had no direct effect on the reverse transcriptase activity of HIV. Saponin B1 also inhibited HIV-induced cell fusion in the MOLT-4 cell system. The results of this study suggest that soybean saponins, especially saponin B1, have inhibitory activity against HIV infection.
authors.affiliation: Department of Virology and Parasitology, Yamaguchi University School of Medicine, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Nakashima
authors.forename: K
authors.initials: K
authors.lastname: Okubo
authors.forename: Y
authors.initials: Y
authors.lastname: Honda
authors.forename: T
authors.initials: T
authors.lastname: Tamura
authors.forename: S
authors.initials: S
authors.lastname: Matsuda
authors.forename: N
authors.initials: N
authors.lastname: Yamamoto
mesh_terms: D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology*; D015496:CD4-Positive T-Lymphocytes / Q000382:microbiology; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D003864:Depression, Chemical; D006027:Glycosides / Q000494:pharmacology; D006678:HIV / Q000187:drug effects* / Q000472:pathogenicity; D015368:Human T-lymphotropic virus 1 / Q000502:physiology; D006801:Humans; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology*; D013025:Glycine max / Q000032:analysis; D014774:Virulence / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D006027:Glycosides; D012503:Saponins