Evidence against involvement of the human erythrocyte plasma membrane Ca2+-ATPase in Ca2+-dependent K+ transport.
Two tests were performed to assess the relationship between the Ca2+-activated K+ channel and the Ca2+-pumping ATPase in human erythrocytes. Antibodies against the purified ATPase inhibited the ATPase in resealed erythrocytes, but had no effect on the K+ channel (as assessed by Rb+ efflux). Reconstituted liposomes containing the purified active Ca2+-pumping ATPase showed no Ca2+-activated Rb+ influx. Both of these results suggest that some molecule other than the Ca2+-ATPase is responsible for the K+ channel.
Verma
A K
AK
Penniston
J T
JT
D000906:Antibodies; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000276:immunology / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000201:enzymology*; D006801:Humans; D007473:Ion Channels / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism*; D012413:Rubidium / Q000378:metabolism
D000906:Antibodies; D007473:Ion Channels; D000252:Calcium-Transporting ATPases; D012413:Rubidium; D011188:Potassium; D002118:Calcium