Chromosomal R-banding with a monoclonal antidouble-stranded DNA antibody.
A monoclonal anti-DNA antibody (HB2) specific for poly dG- poly dC nucleotides was used to stain metaphasic lymphocyte or amniotic cell human chromosomes. HB2 fixation was revealed using either a peroxidase- or a rhodamine-labeled anti-mouse immunoglobulin antiserum. The staining pattern of the chromosomes was dependent on the HB2 concentration: R-banding could be observed at high antibody dilution. Previous trypsinization of metaphasic preparations demonstrated a precise and reproducible typical R-banding independent of the HB2 concentration. This technique appears to be an interesting alternative to other R-banding procedures. The specificity of the antibody allows a better understanding of the biochemical mechanism of R-banding.
Magaud
J P
JP
Rimokh
R
R
Brochier
J
J
Lafage
M
M
Germain
D
D
D000653:Amniotic Fluid / Q000166:cytology; D000911:Antibodies, Monoclonal; D002871:Chromosome Banding / Q000379:methods*; D002900:Chromosomes, Human, 1-3; D004247:DNA / Q000276:immunology; D005260:Female; D006801:Humans; D007621:Karyotyping; D008214:Lymphocytes / Q000648:ultrastructure; D008677:Metaphase; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal; D004247:DNA