[Cytological analysis of the protein component of the nuclear chromatin in rat sympathetic neurocytes in postnatal ontogeny. II. The age-related changes in the nonhistone chromosomal proteins detectable by fast green and the matrix activity before and after extraction of the weakly bound fraction].
A cytophotometrical study was made of Fast Green FCF binding by nuclear non-histone proteins of the rat superior ganglion neurons at pH 2.6 (1 day, 1 week, 1 and 5 months, and 2.5 years after birth, a. b.). An increased dye binding by fraction NHP, stable in 0.35 M NaCl, was seen during the terminal cytodifferentiation (between days 7 and 14 a. b.). The extraction of a loosely bound fraction of non-histone proteins caused a decrease in the transcription level evaluated by the Moore method, throughout the whole postnatal ontogenesis, and no effect of extraction was noticed in respect of the AS-staining of nuclear histones.
Grigor'eva
A V
AV
Iarygin
V N
VN
D000375:Aging; D000818:Animals; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure*; D002868:Chromosomal Proteins, Non-Histone / Q000302:isolation & purification / Q000378:metabolism*; D005728:Ganglia, Sympathetic / Q000378:metabolism / Q000648:ultrastructure*; D006657:Histones / Q000378:metabolism; D008086:Lissamine Green Dyes; D009474:Neurons / Q000378:metabolism / Q000648:ultrastructure*; D009562:Nissl Bodies / Q000378:metabolism / Q000648:ultrastructure*; D051381:Rats; D012834:Silver; D013194:Staining and Labeling / Q000379:methods; D014158:Transcription, Genetic
D002868:Chromosomal Proteins, Non-Histone; D006657:Histones; D008086:Lissamine Green Dyes; C000212:ammoniacal silver; D012834:Silver; C007704:Fast Green FCF
Tsitologicheskiĭ analiz belkovogo komponenta iadernogo khromatina simpaticheskikh nevrotsitov krysy v postnatal'nom ontogeneze. II. Vozrastnye izmeneniia negistonovykh iadernykh belkov, vyiavliaemye prochnym zelenym, i matrichnaia aktivnost' do i posle ékstraktsii slabosviazannoĭ fraktsii.