Phagemid VPCS vectors for priming, cloning and sequencing.
A phagemid was adapted for use as the vector in the vector-primer-cloner-sequencer cloning system. The use of this new vector markedly expanded the utility of this technology for the construction of cDNA libraries. Technological advantages and new capabilities include: (1) a greater number of unique restriction sites within the polylinker region; (2) the ability to produce single-stranded templates for nucleotide sequencing, and (3) a convenient means to synthesize strand-specific hybridization probes. With the use of this cloning system, a rat liver cDNA library (8.56 x 10(5) recombinants from 1 microgram of poly(A)+ RNA) was rapidly (in two days) constructed.
Krawetz
S A
SA
Department of Medical Biochemistry, Faculty of Medicine, University of Calgary, Alberta, Canada.
Sellos
D
D
Wong
N C
NC
Dixon
G H
GH
D003001:Cloning, Molecular / Q000379:methods*; D004274:DNA, Recombinant; D015723:Gene Library; D005822:Genetic Vectors; D010957:Plasmids; D015183:Restriction Mapping
D004274:DNA, Recombinant