Inhibitory effect of a lichen polysaccharide sulfate, GE-3-S, on the replication of human immunodeficiency virus (HIV) in vitro.
A sulfate (GE-3-S) prepared by chlorosulfonic acid treatment of GE-3, a partially acetylated beta(1----6) glucan of the lichen Umbilicaria esculenta, inhibited the cytopathic effect of human immunodeficiency virus (HIV) and suppressed the HIV-antigen expression in Molt-4 (clone 8) cells. GE-3-S also suppressed the giant cell formation of HIV-infected Molt-4 cells, and inhibited HIV-induced plaque formation by 50% at the dose of 19.5 micrograms/ml and completely at 250 micrograms/ml in MT4 cells. GE-3-S had no direct effect on the reverse transcriptase of HIV.
Hirabayashi
K
K
Iwata
S
S
Ito
M
M
Shigeta
S
S
Narui
T
T
Mori
T
T
Shibata
S
S
D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002478:Cells, Cultured; D006678:HIV / Q000187:drug effects* / Q000502:physiology; D011134:Polysaccharides / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects*
D000998:Antiviral Agents; D011134:Polysaccharides