Identification of a human ribosomal protein mRNA with increased expression in colorectal tumours.
A human ribosomal protein cDNA was selected from a normal colon cDNA library on the basis of overexpression in familial adenomatous polyposis. Nucleotide sequence analysis was used to identify this cDNA as corresponding to the human equivalent of the rat ribosomal protein L31 (HL31). We have quantified the expression of HL31 mRNA in colorectal tumours and found overexpression in 23 out of 23 cases. Our results indicate that HL31 is associated with a malfunction of normal growth regulatory mechanisms in these tumours, and suggest a role for HL31 in proliferation and neoplasia.
Chester
K A
KA
Biochemistry Department, Charing Cross & Westminster Medical School, London, U.K.
Robson
L
L
Begent
R H
RH
Talbot
I C
IC
Pringle
J H
JH
Primrose
L
L
Macpherson
A J
AJ
Boxer
G
G
Southall
P
P
Malcolm
A D
AD
D000230:Adenocarcinoma / Q000235:genetics*; D000595:Amino Acid Sequence; D001345:Autoradiography; D001483:Base Sequence; D015179:Colorectal Neoplasms / Q000235:genetics*; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics*; D012269:Ribosomal Proteins / Q000235:genetics*
D012333:RNA, Messenger; D012269:Ribosomal Proteins; D004247:DNA