Developmentally expressed O-acetyl ganglioside GT3 in fetal rat cerebral cortex.
Monoclonal antibody M6704, established against the chick neural tube, was shown to recognize a trisialosyl residue, NeuAc alpha 2-8NeuAc alpha 2-8NeuAc alpha 2-3-R of C-series gangliosides. Using this antibody, the developmental changes of C-series gangliosides in fetal rat cerebral cortex have been examined. Two dimensional thin layer chromatography (TLC) enzyme-immunostaining analysis revealed that alkali treatment resulted in a great increase in GT3 that amounted to more than 85% of the total GT3 detected. The alkalilabile form was easily degraded to form GT3 by the action of the receptor-destroying enzyme of influenza C virus, sialate O-acetylesterase, indicating that the antigen was most probably 9-O-acetyl-NeuAc containing GT3. The ganglioside was highly enriched at the 14th gestation day, gradually decreased, and was not detected in adult rat cerebral cortex.
Hirabayashi
Y
Y
Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Science, Japan.
Hirota
M
M
Suzuki
Y
Y
Matsumoto
M
M
Obata
K
K
Ando
S
S
D000375:Aging / Q000378:metabolism*; D000818:Animals; D000911:Antibodies, Monoclonal; D002540:Cerebral Cortex / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D005314:Embryonic and Fetal Development; D005732:Gangliosides / Q000378:metabolism*; D006028:Glycosphingolipids / Q000378:metabolism*; D007790:Lactosylceramides / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D000911:Antibodies, Monoclonal; D005732:Gangliosides; D006028:Glycosphingolipids; D007790:Lactosylceramides; C047989:trisialosyllactosylceramide