title: Cobalamin and its binding protein in rat milk.
abstract: Cobalamin and its binding protein, haptocorrin, are present in rat milk throughout the lactation period. The concentration of cobalamin is approximately 0.3-times the concentration of the unsaturated binding protein. The concentration of the unsaturated cobalamin-binding protein varies between 18 nmol l-1 and 16 nmol l-1. The binding protein has a Stokes radius of 2.49 nm when saturated with cobalamin and 2.61 nm when unsaturated. It binds cobalamin over a broad range of pH and is able to bind cobinamide also. With immunohistochemistry, we find haptocorrin immunoreactivity in the mammary glands and in the sebaceous glands of the nipple.
authors.affiliation: Department B, University of Copenhagen, Denmark.
authors.forename: L
authors.initials: L
authors.lastname: Raaberg
authors.forename: E
authors.initials: E
authors.lastname: Nexø
authors.forename: S S
authors.initials: SS
authors.lastname: Poulsen
authors.forename: L
authors.initials: L
authors.lastname: Tollund
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D005260:Female; D007150:Immunohistochemistry; D007774:Lactation / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000378:metabolism; D008892:Milk / Q000032:analysis*; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains; D014155:Transcobalamins / Q000032:analysis* / Q000378:metabolism; D014805:Vitamin B 12 / Q000032:analysis* / Q000378:metabolism
chemical_list: D014155:Transcobalamins; D014805:Vitamin B 12