Sulfonium salts as derivatizing agents. 2. Determination of theophylline in plasma by automated gas chromatography.
An automated gas chromatographic method for determination of theophylline in plasma is described. A novel feature of this assay is the use of triethylsulfonium hydroxide as an on-column alkylating agent. A simple extraction procedure together with automated sample injection and data processing has facilitated processing of large numbers of samples generated in pharmacokinetic studies. The between-run coefficient of variation was 2.2% at 5 micrograms/ml (n = 5) and 3.9% at 15 micrograms/ml (n = 6). Within-run coefficient of variation was 2.4% at 5 micrograms/ml (n = 9) and 3.3% at 15 micrograms/ml (n = 6). Accuracy was 105% at a concentration of 5 micrograms/ml and 102% at 15 micrograms/ml.
Lee
B L
BL
Department of Medicine, University of California, San Francisco General Hospital Medical Center 94110.
Jacob
P
P
Benowitz
N L
NL
D000478:Alkylation; D001331:Automation; D002849:Chromatography, Gas / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D009861:Onium Compounds; D013452:Sulfonium Compounds / Q000032:analysis*; D013806:Theophylline / Q000031:analogs & derivatives / Q000097:blood*
D009861:Onium Compounds; D013452:Sulfonium Compounds; D013806:Theophylline