Binding and release of C3 from Leishmania donovani promastigotes during incubation in normal human serum.
We have examined the nature and extent of C3 deposition on Leishmania donovani, strain 1S, clone 2D, promastigotes. Total molecules of C3 bound/parasite after 60 min was similar for parasites incubated in normal human serum, normal human serum adsorbed to remove natural antibody, or either serum source chelated with Mg-EGTA to limit activation to the alternative pathway. A comparison of parasites grown to early, mid, late-log or stationary phases revealed no difference in the extent and kinetics of C3 binding. C3 bound covalently to the parasite primarily through a hydroxylamine resistant (putatively amide) linkage. Of the bound C3, 75% was present as hemolytically inactive iC3b. Nearly 50% of the bound C3 was spontaneously released within 30 min at 37 degrees C. This spontaneous release was due to an unusual proteolytic cleavage event that released C3 from the C3 acceptor on the parasite surface. These results define and characterize the unusual features of C3 binding to L. donovani promastigotes during incubation in serum.
Puentes
S M
SM
Clinical Immunology Section, National Institutes of Allergy and Infectious Diseases, Bethesda, MD 20892.
Dwyer
D M
DM
Bates
P A
PA
Joiner
K A
KA
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D001665:Binding Sites; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism; D003167:Complement Activation; D003176:Complement C3 / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D007893:Leishmania donovani / Q000254:growth & development / Q000276:immunology* / Q000378:metabolism; D008970:Molecular Weight
D003176:Complement C3