title: cDNA cloning of human calpastatin: sequence homology among human, pig, and rabbit calpastatins.
abstract: cDNA of human calpastatin, an inhibitor protein specific for calpain (EC 3.4.22.17; Ca2(+)-dependent cysteine proteinase) was isolated by screening of a library prepared from human liver mRNA with pig calpastatin cDNA fragment as a probe. The primary structure of human calpastatin was deduced from the nucleotide sequence of the cDNA and compared with that of pig and rabbit calpastatins already reported. Human calpastatin consisted of 673 amino acid residues and had 78% and 77% identity to pig or rabbit calpastatins, respectively. Human calpastatin had a domain structure with four internally repetitive sequences and one N-terminal non-homologous sequence like the other calpastatins. Human calpastatin had two deletions, 22 and 13 residues long in domain L and domain 1, respectively, compared to pig or rabbit calpastatins.
authors.affiliation: Pharmaceutical Research Laboratories, Takara Shuzo Co., Ltd., Kyoto, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Asada
authors.forename: Y
authors.initials: Y
authors.lastname: Ishino
authors.forename: M
authors.initials: M
authors.lastname: Shimada
authors.forename: T
authors.initials: T
authors.lastname: Shimojo
authors.forename: M
authors.initials: M
authors.lastname: Endo
authors.forename: F
authors.initials: F
authors.lastname: Kimizuka
authors.forename: I
authors.initials: I
authors.lastname: Kato
authors.forename: M
authors.initials: M
authors.lastname: Maki
authors.forename: M
authors.initials: M
authors.lastname: Hatanaka
authors.forename: T
authors.initials: T
authors.lastname: Murachi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000235:genetics*; D002154:Calpain / Q000037:antagonists & inhibitors; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015723:Gene Library; D006801:Humans; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D011061:Poly A / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
chemical_list: D002135:Calcium-Binding Proteins; D012333:RNA, Messenger; D011061:Poly A; C033668:calpastatin; D004247:DNA; D002154:Calpain