Constitutive in vitro binding of nuclear proteins to the 5'-flanking region of 6-16, a human gene inducible by alpha, beta-interferons.
Proteins in nuclear extracts of HeLa cells that constitutively bound in vitro to three regions upstream of the interferon-inducible gene 6-16 were separated partially by chromatography on DEAE-Sepharose. Region one, a CCAAT box in the non-coding strand at position --63 to --67, was protected from DNase digestion by the bound protein(s) and was required for transcription in vitro. Region two, a tandem duplication sequence at position --89 to --168 contains two copies of a sequence essential for strong induction of the 6-16 gene by interferon in vitro. Region three, a palindromic sequence at position --449 to --465, not necessary for induction of 6-16 by interferon, was also protected from DNase digestion by nuclear protein(s). Templates with or without regions of two and three were transcribed equally well in extracts from interferon-treated or untreated cells.
Chernajovsky
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Department of Immunology, University of Texas, M.D. Anderson Cancer Center, Houston 77030.
D001483:Base Sequence; D002872:Chromosome Deletion; D004273:DNA, Neoplasm / Q000235:genetics / Q000378:metabolism*; D004742:Enhancer Elements, Genetic; D015870:Gene Expression / Q000187:drug effects*; D005809:Genes, Regulator / Q000187:drug effects; D005822:Genetic Vectors; D006367:HeLa Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D010957:Plasmids; D011401:Promoter Regions, Genetic / Q000187:drug effects; D011485:Protein Binding; D014158:Transcription, Genetic
D004273:DNA, Neoplasm; D007370:Interferon Type I; D009687:Nuclear Proteins