title: Effects of human recombinant interleukin 5 and 3 on the differentiation of cord blood-derived eosinophils and basophils.
abstract: When mononuclear cells from umbilical-cord blood were cultured for 3 weeks, low concentrations of interleukin 3 supported the preferential growth of basophils, with eosinophils comprising a smaller proportion. These basophils contained 0.15-0.3 microgram histamine per 10(6) cells, and released histamine by the IgE-dependent and -independent stimuli. Interleukin 5 increased the number and proportion of eosinophils in a dose-dependent manner without affecting the proliferation of other cell types in the interleukin 3-supplemented cultures. These cultured eosinophils could be activated by platelet-activating factor.
authors.forename: M
authors.initials: M
authors.lastname: Ebisawa
authors.forename: H
authors.initials: H
authors.lastname: Saito
authors.forename: D C
authors.initials: DC
authors.lastname: Reason
authors.forename: K
authors.initials: K
authors.lastname: Ohno
authors.forename: T
authors.initials: T
authors.lastname: Sudo
authors.forename: K
authors.initials: K
authors.lastname: Kurihara
authors.forename: T
authors.initials: T
authors.lastname: Nagakura
authors.forename: Y
authors.initials: Y
authors.lastname: Iikura
mesh_terms: D001491:Basophils / Q000166:cytology*; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D004804:Eosinophils / Q000166:cytology*; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D007377:Interleukin-3 / Q000494:pharmacology*; D015848:Interleukin-5 / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D007377:Interleukin-3; D015848:Interleukin-5; D011994:Recombinant Proteins