Lactate dehydrogenase isoenzyme composition of human platelets.
The LDH isoenzyme composition of 12 platelet preparations was determined by electrophoresis. The mean (+/- SD) percentages of LDH-1, LDH-2, LDH-3, LDH-4, and LDH-5 were 16.6 +/- 1.7, 30.1 +/- 1.0, 34.2 +/- 1.3, 18.2 +/- 1.3, and 0.9 +/- 1.1, respectively. In comparison with previous data, these data show identical ranking of the prevalence of each isoenzyme but significantly different percentages, particularly of LDH-1 and LDH-4. The release of platelet LDH by freezing and thawing differed little from that by homogenization.
Miyada
D S
DS
Department of Pathology, University of California, Irvine Medical Center, Orange 92668.
Fagin
A
A
Pirkle
H
H
Ocariz
J
J
Vaziri
N D
ND
D001792:Blood Platelets / Q000201:enzymology*; D004586:Electrophoresis / Q000379:methods; D006801:Humans; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000097:blood*; D013696:Temperature
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase