Cytotoxicity of cholesterol oxidase to cells of hypercholesterolemic guinea pigs.
1. A high cholesterol diet caused guinea pig erythrocytes to become sensitive to lysis by cholesterol oxidase (CO), a protein not hemolytic to normal cells. 2. Lysis was associated with conversion of membrane cholesterol to its oxidation product (delta-4-cholesten-3-one). 3. Intravenous injection of CO to hypercholesterolemic guinea pigs produced a reduction in serum cholesterol, but was not lethal as it was in rabbits. 4. Homogenized spleen, liver and kidney from the hyperlipidemic animals were sensitive to in vitro cholesterol oxidation while tissues from non-lipemic animals were resistant to modification.
Linder
R
R
Hunter College School of Health Sciences, New York, NY.
Bernheimer
A W
AW
Cooper
N S
NS
Pallias
J D
JD
D015096:3-Hydroxysteroid Dehydrogenases / Q000633:toxicity*; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002789:Cholesterol Oxidase / Q000633:toxicity*; D002791:Cholesterol, Dietary; D004912:Erythrocytes / Q000187:drug effects*; D006168:Guinea Pigs; D006937:Hypercholesterolemia / Q000097:blood* / Q000209:etiology; D007668:Kidney / Q000378:metabolism; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D013154:Spleen / Q000378:metabolism
D002791:Cholesterol, Dietary; D015096:3-Hydroxysteroid Dehydrogenases; D002789:Cholesterol Oxidase