Probable role of both sulfhydryls and prostaglandins in gastric mucosal protection induced by S-adenosylmethionine.
The role of both sulfhydryl groups and endogenous prostaglandins in the protective effect of S-adenosylmethionine against ethanol-induced gastric mucosal damage was studied in rats. Drugs were administered subcutaneously or intragastrically to fasted rats 30 or 60 min before 100% ethanol (1 ml/rat), and mucosal lesions were measured planimetrically 1 h later. The gastric mucosal protection given by S-adenosylmethionine or by 20% ethanol (adaptive protection) was significantly diminished by pretreatment of rats with the sulfhydryl blocker iodoacetamide or with the cyclooxygenase inhibitor indomethacin. The protective effect of S-adenosylmethionine could be totally abolished only by pretreatment with the combination of iodoacetamide and indomethacin. Our present data suggest that endogenous release of prostaglandins and sulfhydryl groups may play a role in the protective actions of both S-adenosylmethionine and 20% ethanol (adaptive protection) against ethanol-induced gastric mucosal damage.
Gutiérrez-Cabano
C A
CA
Dept. of Surgical Pathology II, School of Medical Sciences, University of Rosario, Argentina.
D000818:Animals; D000431:Ethanol / Q000008:administration & dosage; D005260:Female; D005753:Gastric Mucosa / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D007213:Indomethacin / Q000008:administration & dosage; D007460:Iodoacetamide / Q000008:administration & dosage; D008297:Male; D011453:Prostaglandins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012436:S-Adenosylmethionine / Q000008:administration & dosage*; D013438:Sulfhydryl Compounds / Q000378:metabolism*
D011453:Prostaglandins; D013438:Sulfhydryl Compounds; D000431:Ethanol; D012436:S-Adenosylmethionine; D007213:Indomethacin; D007460:Iodoacetamide