Establishment of four mouse mastocytoma cell lines.
Four mastocytoma cell lines were isolated from four different mouse mastocytoma tumors. The tumors had been induced in mice treated with tetramethylpentadecane (pristane) and infected with Abelson murine leukemia virus. The cell lines have been carried in culture for over a year and can induce tumors when injected into the mouse strain in which the tumor originated. The cells contain histamine, have high affinity IgE receptors and release histamine by IgE, immune complex or ionophore A23187-induced reactions. This histamine release reaction requires Ca2+, is optimal at 37 degrees C, and is blocked by a number of metabolic inhibitors. There is no requirement for phosphatidylserine. Cloned sublines have been obtained which will be useful for Fc epsilon R, Fc gamma R; and histamine release studies.
Barsumian
E L
EL
McGivney
A
A
Basciano
L K
LK
Siraganian
R P
RP
D000818:Animals; D000941:Antigens / Q000502:physiology; D000001:Calcimycin / Q000494:pharmacology; D002460:Cell Line; D002999:Clone Cells; D006636:Histamine Release; D007621:Karyotyping; D008407:Mast Cells / Q000276:immunology; D012515:Mast-Cell Sarcoma / Q000139:chemically induced / Q000473:pathology*; D051379:Mice; D008815:Mice, Inbred Strains; D017455:Receptors, IgE; D017452:Receptors, IgG; D011971:Receptors, Immunologic / Q000276:immunology
D000941:Antigens; D017455:Receptors, IgE; D017452:Receptors, IgG; D011971:Receptors, Immunologic; D000001:Calcimycin