title: On the presence and localization of epidermal and nerve growth factors in human whole saliva.
abstract: Using antibodies to mouse submandibular epidermal growth factor (EFG) and nerve growth factor (NGF), immunohistochemical studies were performed on sections of flash-frozen human whole saliva in both light and transmission electron microscopy. Light microscopy showed the presence of overall network-like immunoreactions in both the EGF and NGF antibody-treated sections. Electron microscopy showed clearly detectable ultrastructural reaction patterns for both growth factor antibodies. The individual structural elements were more distinct for the EGF antibody-treated sections, in which the reaction elements had approximate diameters of 0.05 micron. In the NGF antibody-treated sections the corresponding approximate diameters were 0.02 micron. In both the EGF and NGF antibody-treated sections heavily stained bacteria-like particles were also frequently observed.
authors.affiliation: Department of Prosthetic Dentistry, Faculty of Odontology, University of Lund, Malmö, Sweden.
authors.forename: P O
authors.initials: PO
authors.lastname: Glantz
authors.forename: M A
authors.initials: MA
authors.lastname: Meenaghan
authors.forename: K H
authors.initials: KH
authors.lastname: Hyun
authors.forename: S M
authors.initials: SM
authors.lastname: Wirth
mesh_terms: D000328:Adult; D004815:Epidermal Growth Factor / Q000032:analysis*; D005260:Female; D005615:Freezing; D006651:Histocytochemistry; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D009414:Nerve Growth Factors / Q000032:analysis*; D012463:Saliva / Q000032:analysis*
chemical_list: D009414:Nerve Growth Factors; D004815:Epidermal Growth Factor