[Detection of c-sis transcripts and PDGF-like products by in situ hybridization and immunohistochemical study].
To clarify the relationship between oncogene c-sis expression and tumorigenesis in human osteosarcoma, in situ hybridization and immunohistochemical studies were performed to detect c-sis mRNA and PDGF-like protein partially consisting of c-sis product. Formalin fixed-paraffin embedded sections of eight cases of osteosarcoma of bone were examined. Three osteosarcomas highly expressed c-sis mRNA with a fine granular pattern in their cytoplasms. Five osteosarcomas, including three cases with c-sis expression, contained PDGF-like protein in their cytoplasms. These results suggested that c-sis oncogene and PDGF-like protein were closely related to tumorigenesis in human osteosarcoma. The DNA-mRNA in situ hybridization technique applied by the author is as efficient as the immunohistochemical method in cancer research.
Nakajima
H
H
Department of Orthopaedic Surgery, St. Marianna University School of Medicine, Kanagawa, Japan.
D001859:Bone Neoplasms / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D012516:Osteosarcoma / Q000235:genetics* / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D014158:Transcription, Genetic
D010982:Platelet-Derived Growth Factor