An improved boiling method for the preparation of bacterial plasmid and phage DNA.
A streamlined, reproducible boiling method for preparing plasmid as well as phage replicative form DNA is described. Both quantity and quality of DNA purified are sufficient for restriction enzyme analysis and sequencing. A small loopful of bacteria will provide enough DNA for several experiments, and multiple samples can be processed and prepared for digestion or sequencing in under 20 minutes. DNA preparations are stable for at least 6 months at -20 degrees C.
Ortlepp
S A
SA
Amersham International, United Kingdom.
D001419:Bacteria / Q000235:genetics; D001435:Bacteriophages / Q000235:genetics; D001483:Base Sequence; D004279:DNA, Viral / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D010957:Plasmids; D015183:Restriction Mapping
D004279:DNA, Viral