[The action of dopamine and serotonin on the cells of an isolated spinal cord ganglion of the rat].
The effect of bath application of dopamine (DA) and serotonin (HT) was studied in the isolated perfused dorsal root ganglia (DRG) of 30-36 days old rats by means of intracellular technique. 92% of investigated cells responded to the application of DA and 87%--to HT. DA and HT evoked depolarization in most of cells (64.6 and 73.7% of cells, respectively). Responses were dose-dependent and reversible. Depolarization caused by the DA and HT application was accompanied by a decrease in the input resistance (Rm) and alteration of the action potential shape. Hyperpolarization was followed by an increase of Rm. It was demonstrated that both DA and HT influenced predominantly DRG neurons with membrane biophysical characteristics inherent in small cells. Possibility of modulation of the afferent impulsation on the level of the primary sensory neurons is supposed.
Molokanova
E A
EA
Tamarova
Z A
ZA
D000200:Action Potentials / Q000187:drug effects / Q000502:physiology; D000818:Animals; D004298:Dopamine / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004553:Electric Conductivity / Q000187:drug effects / Q000502:physiology; D004558:Electric Stimulation; D005727:Ganglia, Spinal / Q000187:drug effects* / Q000502:physiology; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D008839:Microelectrodes; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D010477:Perfusion / Q000379:methods; D051381:Rats; D012701:Serotonin / Q000494:pharmacology*
D012701:Serotonin; D004298:Dopamine
Deĭstvie dofamina i serotonina na kletki izolirovannogo spinno-mozgovogo gangliia krys.