Glutamine synthetase in Lupinus luteus. Identification and preliminary characterization of nodule-specific cDNA clone.
Two glutamine synthetase (GS) cDNA clones from L. luteus were identified and characterized. The nucleotide sequence analysis proved that they represent highly homologous but distinct mRNA species. Northern blot hybridization revealed that pc LINGS encodes the nodule-specific subunit of the GS while pcLIGS1 represents the nonspecific one present in nodule tissue as well as in uninfected roots.
Boroń
L
L
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznaán, Poland.
Szczygłowski
K
K
Konieczny
A
A
Legocki
A B
AB
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D007887:Fabaceae / Q000201:enzymology* / Q000235:genetics; D005974:Glutamate-Ammonia Ligase / Q000235:genetics*; D008969:Molecular Sequence Data; D010946:Plants, Medicinal; D012333:RNA, Messenger / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D012333:RNA, Messenger; D004247:DNA; D005974:Glutamate-Ammonia Ligase