Conjugation system of IncC plasmid RA1, and the interaction of RA1 pili with specific RNA phage C-1.
RA1 was the only IncC plasmid that was slightly temperature-sensitive for replication and transfer. At 30 degrees C, RA1 determined constitutive synthesis of conjugative pili and yet was transfer-repressed. Attachment of shaft-adsorbing RNA phage C-1 virions prevented the probable retraction of pili under heat stimulus (55 degrees C). Electron microscopy showed single adsorbed virions at pilus bases where they were thought to have stopped retraction.
Bradley
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Faculty of Medicine, Memorial University of Newfoundland, St. John's, Canada.
D000333:Aeromonas / Q000235:genetics / Q000648:ultrastructure; D003090:Coliphages / Q000235:genetics / Q000648:ultrastructure; D003227:Conjugation, Genetic; D010861:Fimbriae, Bacterial / Q000378:metabolism* / Q000648:ultrastructure; D008854:Microscopy, Electron; D010641:Phenotype; D011815:R Factors / Q000235:genetics* / Q000502:physiology; D012317:RNA Phages / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D013696:Temperature