Epiderstatin, a new inhibitor of the mitogenic activity induced by epidermal growth factor. I. Taxonomy, fermentation, isolation and characterization.
Inhibitors of mitogenic activity induced by epidermal growth factor (EGF) were screened from culture broths of soil microorganisms. A strain of actinomycetes has been found to produce a new glutarimide antibiotic named epiderstatin which inhibits the incorporation of [3H]thymidine into quiescent animal cells stimulated by EGF. Taxonomic studies have revealed that the producing strain belongs to a subspecies of Streptomyces pulveraceus, thus the name, Streptomyces pulveraceus subsp. epiderstagenes was given to this strain. The molecular formula (C15H20N2O4) and UV profile (lambda max 295 nm) of the antibiotic are distinct from other known antibiotics. It inhibited the incorporation of [3H]thymidine into quiescent cells stronger than into growing cells.
Osada
H
H
Antibiotics Laboratory, RIKEN, Institute of Physical and Chemical Research, Saitama, Japan.
Sonoda
T
T
Kusakabe
H
H
Isono
K
K
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000302:isolation & purification*; D002478:Cells, Cultured; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004815:Epidermal Growth Factor / Q000037:antagonists & inhibitors*; D005285:Fermentation; D009682:Magnetic Resonance Spectroscopy; D008855:Microscopy, Electron, Scanning; D010880:Piperidines / Q000302:isolation & purification*; D010881:Piperidones / Q000302:isolation & purification* / Q000494:pharmacology; D011728:Pyridones / Q000302:isolation & purification* / Q000494:pharmacology; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013171:Spores, Bacterial / Q000648:ultrastructure; D013302:Streptomyces / Q000145:classification* / Q000378:metabolism
D000903:Antibiotics, Antineoplastic; D010880:Piperidines; D010881:Piperidones; D011728:Pyridones; C061874:epiderstatin; D004815:Epidermal Growth Factor