title: Origin, kinetics of circulation and fate in vivo of the major excretory-secretory product of Acanthocheilonema viteae.
abstract: The excretions-secretions (E-S) of Acanthocheilonema viteae consist mainly of one product, molecular weight 62kDa. This molecule is synthesized during the vertebrate phase of the parasite life-cycle and is first detectable in the E-S of L4 parasites. It is cross-reactive with E-S of human filarial parasites as a consequence of possessing a phosphorylcholine (PC) moiety. The 62 kDa molecule has been employed as a model for the study of the origin and fate of filarial E-S. Immunohistological analysis has shown the molecule to be located predominantly in the parasite gut. Transplantation of adult female [35S] methionine pulsed worms into uninfected jirds resulted in the radio-labelled secreted 62 kDa antigen being detected in the bloodstream within 4 h by SDS-PAGE/immunoprecipitation analysis. The systemic half-life of the molecule as estimated by clearance of injected, purified 125I-labelled material was measured in naive and infected jird hosts. It was reduced from 2-7 h in naive animals to less than 30 min in 4-10 week infected rodents, a finding which correlated with clearance of antigen by antibody in the infected group. In animals infected for longer time periods the serum half-life returned to the values observed in naive jirds. The idea that this change in half-life may reflect differences in the nature of 62 kDa antigen containing circulating immune complexes as infection progresses is discussed. The 125I-labelled antigen is predominantly removed from the circulation via the liver and ultimately excreted in the urine in a non-antigenic form. This work provides the first description of the origin, kinetics of circulation and fate of a defined filarial E-S product and may aid in determining the function and assessing the diagnostic utility of PC-bearing E-S components.
authors.affiliation: Division of Parasitology, National Institute for Medical Research, Ridgeway, Mil Hill, London.
authors.forename: W
authors.initials: W
authors.lastname: Harnett
authors.forename: M J
authors.initials: MJ
authors.lastname: Worms
authors.forename: A
authors.initials: A
authors.lastname: Kapil
authors.forename: M
authors.initials: M
authors.lastname: Grainger
authors.forename: R M
authors.initials: RM
authors.lastname: Parkhouse
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis*; D003429:Cross Reactions; D004153:Dipetalonema / Q000032:analysis* / Q000276:immunology; D004154:Dipetalonema Infections / Q000276:immunology / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005368:Filariasis / Q000378:metabolism*; D005849:Gerbillinae; D015801:Helminth Proteins / Q000032:analysis*; D007700:Kinetics; D008970:Molecular Weight; D010767:Phosphorylcholine / Q000032:analysis; D011233:Precipitin Tests
chemical_list: D000947:Antigens, Helminth; D015801:Helminth Proteins; D010767:Phosphorylcholine