Cell surface expression of an in vitro recombinant class II/class I major histocompatibility complex gene product.
Chimeric histocompatibility genes encoding the amino-terminal (beta 1) domain of the class II Ak beta polypeptide and the carboxy-terminal (C2, transmembrane, and intracytoplasmic) domains of either the class I H-2Ld or H-2Dd molecules were stably introduced into mouse L cells. Although both were transcribed, only 5' Ak beta/3' H-2Dd transformants had significant cell membrane expression of a 30-40 kd, heterogeneous glycoprotein containing Ak beta 1 and H-2Dd (C2) serological epitopes. These transformants had a unique pattern of reactivity with monoclonal antibodies previously identified as requiring the Ak beta 1 domain for recognition of complete I-A molecules. These results allow new insight into the structural requirements for cell surface expression of proteins and provide unique cellular reagents for the dissection of humoral and cell-mediated recognition of MHC molecules.
McCluskey
J
J
Germain
R N
RN
Margulies
D H
DH
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis* / Q000235:genetics; D004586:Electrophoresis; D006183:H-2 Antigens / Q000032:analysis* / Q000235:genetics; D007739:L Cells / Q000276:immunology; D008285:Major Histocompatibility Complex; D051379:Mice; D012313:RNA / Q000032:analysis; D011995:Recombination, Genetic; D014158:Transcription, Genetic; D014170:Transformation, Genetic
D000954:Antigens, Surface; D006183:H-2 Antigens; D012313:RNA