title: Influence of the H-2u haplotype on immune function in F1 hybrid mice. I. Antigen presentation.
abstract: Previously, we reported that myelin basic protein (MBP) peptide 1-37 contains an encephalitogenic epitope for PL/J mice, and MBP peptide 89-169 is encephalitogenic for SJL/J mice. (SJPL)F1 hybrid mice do not respond to immunization with these peptides in a co-dominant manner because the encephalitogenic response to peptide 1-37 dominates. To examine this phenomenon more closely, we tested the ability of MBP-primed parental or F1 T cells to respond to MBP or MBP peptides in the context of PL, SJL, or F1 antigen-presenting cells (APC). It was found that the F1 T cells responded to either the protein or the peptides when these were presented in the context of F1 or PL APC. However, F1 T cells would not respond to MBP in the context of SJL APC, although the latter cells were functionally intact. This effect was not antigen-specific because SJL APC would not present ovalbumin or PPD to primed F1 T cells. F1 T cells from mice immune to the strongly antigenic bacterium Listeria monocytogenes responded to bacterial antigens presented by SJL APC, although at a significantly lower level compared to the results obtained when these antigens were presented by F1 or PL APC. This finding implied that unbalanced antigen presentation was a quantitative rather than a qualitative phenomenon. When F1 hybrid mice from other strain combinations were tested, a similar effect was observed whenever one of the parental strains was PL/J. This effect was mapped to the MHC in MHC-congenic B10 mice.
authors.forename: R B
authors.initials: RB
authors.lastname: Fritz
authors.forename: M J
authors.initials: MJ
authors.lastname: Skeen
authors.forename: H K
authors.initials: HK
authors.lastname: Ziegler
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D003433:Crosses, Genetic; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes / Q000235:genetics; D005802:Genes, MHC Class II; D005838:Genotype; D006183:H-2 Antigens / Q000276:immunology*; D006649:Histocompatibility Antigens / Q000235:genetics; D000949:Histocompatibility Antigens Class II; D008089:Listeria monocytogenes / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D004676:Myelin Basic Protein / Q000276:immunology; D010047:Ovalbumin / Q000276:immunology
chemical_list: D000939:Epitopes; D006183:H-2 Antigens; D006649:Histocompatibility Antigens; D000949:Histocompatibility Antigens Class II; C024138:I-E-antigen; D004676:Myelin Basic Protein; D010047:Ovalbumin