title: [Determination of the substrate specificity of Bpu101 restrictase with an unusual recognition segment].
abstract: A new enzyme Bpu10I was isolated from Bacillus pumilus. This enzyme is not an isoschizomer of any known restriction endonucleases. The search of possible recognition sequences was carried out in sequences ABCNiDEF (i = 0.6) on substrate DNA lambda CI857, T7, pBR322. The recognition sequence and cleavage sites of restriction endonuclease Bpu10I have been determined as CCTNAGC. GGANTCG
authors.forename: S Kh
authors.initials: SKh
authors.lastname: Degtiarev
authors.forename: P A
authors.initials: PA
authors.lastname: Zhilkin
authors.forename: G G
authors.initials: GG
authors.lastname: Prikhod'ko
authors.forename: N I
authors.initials: NI
authors.lastname: Rechkunova
authors.forename: V E
authors.initials: VE
authors.lastname: Repin
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D001483:Base Sequence; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D008969:Molecular Sequence Data; D013379:Substrate Specificity
chemical_list: C061882:endodeoxyribonuclease Bpu10I; D015252:Deoxyribonucleases, Type II Site-Specific
vernacular_title: Opredelenie substratnoĭ spetsifichnosti restriktazy BPu101 s neobychnym uchastkom uznavaniia.