Placental form of glutathione S-transferase in normal and diseased human uterine cervical mucosa.
Expression of the human placental form of glutathione S-transferase (GST-pi), an enzyme proposed as a marker for human and experimental neoplasia, was immunohistochemically evaluated in 51 samples of 'normal' and diseased adult human uterine cervix. Five fetal uteri were also studied. GST-pi positivity was detected in 54, 92, 95 and 83% of the 'normal', non-neoplastic, cervical intra-epithelial neoplasia (CIN) and cancer cases respectively. All five fetal uteri and the positive 'normal' adult cases presented cells immunostained for GST-pi throughout the thickness of the mucosa, including the basal layer. Some non-neoplastic conditions like inflammation, repair and metaplasia and some dysplastic and neoplastic lesions showed areas of positively stained cells within an otherwise negative tissue, indicating a phenotypic heterogeneity regarding the enzyme expression. Our results confirm that GST-pi has a fetal character and indicate that it may appear in the adult cervical squamous epithelia under 'normal' or pathological conditions not necessarily linked to the process of carcinogenesis. Therefore it cannot be used as a marker for cervical epithelial neoplasia.
de Camargo
J L
JL
Department of Pathology, Faculty of Medicine, UNESP, Botucatu, Brazil.
Tsuda
H
H
Tatematsu
M
M
Rodrigues
M A
MA
Yamada
M
M
Tzuji
K
K
Ito
N
N
D015415:Biomarkers / Q000032:analysis; D014408:Biomarkers, Tumor / Q000032:analysis; D002584:Cervix Uteri / Q000201:enzymology* / Q000473:pathology; D004848:Epithelium / Q000201:enzymology; D005260:Female; D005982:Glutathione Transferase / Q000032:analysis*; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D009092:Mucous Membrane / Q000201:enzymology; D010920:Placenta / Q000201:enzymology*; D012016:Reference Values; D002577:Uterine Cervical Diseases / Q000201:enzymology* / Q000473:pathology; D002578:Uterine Cervical Dysplasia / Q000201:enzymology* / Q000473:pathology; D002583:Uterine Cervical Neoplasms / Q000201:enzymology* / Q000473:pathology
D015415:Biomarkers; D014408:Biomarkers, Tumor; D007527:Isoenzymes; D005982:Glutathione Transferase