Transforming growth factor-beta induces hemoglobin synthesis in a human erythroleukemia cell line.
We have examined the effects of TGF beta 1 and TGF beta 2 on the HEL human erythroleukemia cell line. It was observed that TGF beta 1 and 2 induced hemoglobin synthesis in these cells without causing a significant negative effect on cell proliferation. The cell surface markers glycophorin A and transferrin receptor that are associated with erythroid differentiation were also increased. This cell line may provide a model system in which to study the regulation of globin gene expression by a physiological growth factor known to act on hemopoietic cells.
Hooper
W C
WC
Division of Host Factors, Centers for Disease Control, Atlanta, GA 30333.
Pruckler
J
J
Jackson
D
D
Evatt
B L
BL
D015152:Blotting, Northern; D002460:Cell Line; D015870:Gene Expression; D005796:Genes / Q000187:drug effects*; D006021:Glycophorins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D006454:Hemoglobins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007700:Kinetics; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism*; D011990:Receptors, Transferrin / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D015291:Transforming Growth Factors / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism*
D006021:Glycophorins; D006454:Hemoglobins; D011990:Receptors, Transferrin; D015291:Transforming Growth Factors