Decreased transport of p-aminohippurate in renal basolateral membranes isolated from rats with acute renal failure.
Transport of D-glucose, p-aminohippurate (organic anion), and tetraethylammonium (organic cation) was studied in the renal basolateral membrane vesicles isolated from rats with acute renal failure (ARF). ARF was induced by a single injection of uranyl nitrate. Carrier-mediated transport of p-aminohippurate, estimated under anion-anion exchange condition, was significantly decreased in basolateral membrane vesicles isolated from ARF rats. In contrast, there were no significant differences in D-glucose and tetraethylammonium uptake between normal and ARF rats. When normal basolateral membrane vesicles were incubated in vitro with uranyl nitrate, no significant inhibition in p-aminohippurate uptake was observed. These results suggest that organic anion transport is decreased in renal basolateral membranes from ARF rats, and this transport dysfunction cannot be explained by the direct interaction of uranyl nitrate with the organic anion carrier.
Inui
K
K
Department of Pharmacy, Faculty of Medicine, Kyoto University, Japan.
Takano
M
M
Maegawa
H
H
Kato
M
M
Hori
R
R
D058186:Acute Kidney Injury / Q000378:metabolism*; D000618:Aminohippuric Acids / Q000493:pharmacokinetics*; D000818:Animals; D001692:Biological Transport; D005947:Glucose / Q000493:pharmacokinetics; D066298:In Vitro Techniques; D007668:Kidney / Q000378:metabolism*; D008297:Male; D008566:Membranes / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013757:Tetraethylammonium Compounds / Q000493:pharmacokinetics; D010130:p-Aminohippuric Acid / Q000493:pharmacokinetics*
D000618:Aminohippuric Acids; D013757:Tetraethylammonium Compounds; D005947:Glucose; D010130:p-Aminohippuric Acid