Histogenetic study of the vestibular ganglion in the developing chick embryo by lectin-horseradish peroxidase methods.
Hatchery eggs of White Leghorn hens were incubated and the vestibular ganglion cells of 3.5- to 6-day-old chick embryos were studied by the lectin-horseradish peroxidase methods (WGA, UEA-1). In the 3.5-day-old chick embryos, the facial-acoustic ganglion mass was recognized close to the otic vesicle. The statoacoustic ganglion reacted to lectins on the rhombencephalic side, indicating that glycoconjugates play a role in the nerve connection between ganglion and rhombencephalon. In the 4-day-old chick embryos, a strong reaction to lectins was observed on the ganglion cell membrane of the geniculate and statoacoustic ganglions, suggesting that glycoconjugates may be related to their differentiation. In 4- and 5-day-old chick embryos, the mesenchymal tissue showed a strong reaction to lectins, suggesting that it plays an important part in the differentiation of the vestibular ganglion.
Yamashita
H
H
Department of Otolaryngology, Yamaguchi University School of Medicine, Japan.
Sekitani
T
T
D000818:Animals; D002642:Chick Embryo / Q000254:growth & development*; D006735:Horseradish Peroxidase; D013997:Time Factors; D014725:Vestibular Nerve / Q000196:embryology*
D006735:Horseradish Peroxidase