BOAA selectively enhances L-glutamate release from guinea pig hippocampal mossy fiber synaptosomes.
The neurotoxic amino acids beta-N-oxalylamino-L-alanine (BOAA) and beta-N-methylamino-L-alanine (BMAA) were evaluated for possible effects on spontaneous and stimulus-evoked release of L-glutamate (L-Glu) and dynorphin A(1-8)-like immunoreactivity (LI) from guinea pig hippocampal mossy fiber synaptosomes. BOAA (200 microM), but not BMAA (1 mM), was found to significantly increase both basal cytosolic and KCl-stimulated vesicular release of L-Glu. Neither BOAA nor BMAA had any effect on dynorphin A(1-8)-LI release from these synaptosomes. This is the first report describing a presynaptic facilitatory action of BOAA upon L-Glu release; an effect which may contribute to the neurotoxic properties of this proposed environmental toxin.
Gannon
R L
RL
Clinical Sciences Division, USAF School of Aerospace Medicine, Brooks AFB, TX 78235-5301.
Terrian
D M
DM
D000409:Alanine / Q000031:analogs & derivatives*; D000599:Amino Acids, Diamino / Q000494:pharmacology; D000818:Animals; D000087522:Cyanobacteria Toxins; D004399:Dynorphins / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D006168:Guinea Pigs; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010446:Peptide Fragments / Q000378:metabolism; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism; D015091:beta-Alanine / Q000031:analogs & derivatives* / Q000494:pharmacology
D000599:Amino Acids, Diamino; D000087522:Cyanobacteria Toxins; D005971:Glutamates; D010446:Peptide Fragments; C001824:beta-N-methylamino-L-alanine; D015091:beta-Alanine; C100126:oxalyldiaminopropionic acid; D018698:Glutamic Acid; D004399:Dynorphins; C026770:dynorphin (1-8); D000409:Alanine