title: Predictions of structural elements for the binding of Hin recombinase with the hix site of DNA.
abstract: Molecular dynamics simulations were coupled with experimental data from biochemistry and genetics to generate a theoretical structure for the binding domain of Hin recombinase complexed with the hix site of DNA. The theoretical model explains the observed sequence specificity of Hin recombinase and leads to a number of testable predictions concerning altered sequence selectivity for various mutants of protein and DNA.
authors.affiliation: Arthur Amos Noyes Laboratory of Chemical Physics, California Institute of Technology, Pasadena 91125.
authors.forename: K W
authors.initials: KW
authors.lastname: Plaxco
authors.forename: A M
authors.initials: AM
authors.lastname: Mathiowetz
authors.forename: W A
authors.initials: WA
authors.lastname: Goddard
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000378:metabolism*; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
chemical_list: D004247:DNA; D004254:DNA Nucleotidyltransferases; C059997:Hin recombinase