Interferon produced endogenously in response to CSF-1 augments the functional differentiation of progeny macrophages.
The role of endogenously produced interferon alpha/beta in the functional maturation of newly derived mononuclear phagocytes was investigated. Addition of highly specific anti-interferon alpha + beta antiserum to murine marrow cultures stimulated with colony-stimulating factor-1 (macrophage growth factor) markedly suppressed the capacity of resulting progeny mononuclear phagocytes to ingest opsonized sheep erythrocytes (EAIgG). This impairment was corrected either by direct addition of interferon alpha + beta at a concentration in excess of that neutralized by the antiserum or by the addition of lesser amounts of interferon (33 U/ml) following removal of the anti-interferon from the cultures. Conditioned media from control colony-stimulating factor-stimulated cultures similarly reversed the impairment of maturation resulting from 5 days of growth in the presence of anti-interferon. This enhancement of EAIgG ingestion reflected upon the interferon activity in the conditioned media and was neutralized by anti-interferon. Lastly, the endogenous interferon was found to enhance EAIgG ingestion by a majority of the mononuclear phagocyte progeny and not by a limited subpopulation.
Moore
R N
RN
Pitruzzello
F J
FJ
Robinson
R M
RM
Rouse
B T
BT
D000818:Animals; D000936:Antigen-Antibody Complex; D001854:Bone Marrow Cells; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000494:pharmacology*; D007372:Interferons / Q000276:immunology*; D008264:Macrophages / Q000166:cytology* / Q000502:physiology; D051379:Mice; D008809:Mice, Inbred C3H; D010587:Phagocytosis; D011961:Receptors, Fc / Q000378:metabolism
D000936:Antigen-Antibody Complex; D003115:Colony-Stimulating Factors; D011961:Receptors, Fc; D007372:Interferons