Biochemical characterization of a sterol mutant plant regenerated from a tobacco callus resistant to a triazole cytochrome-P-450-obtusifoliol-14-demethylase inhibitor.
We report here, for the first time, the biochemical characterization of a plant mutant impaired in sterol biosynthesis. A fertile plant was regenerated from a tobacco callus resistant to LAB170250F, a potent inhibitor of the cytochrome-P450-obtusifoliol-14-demthylase. The resistant callus and the leaves from the regenerated plant are characterized by profound qualitative and quantitative changes in their sterol content. Self-fertilization of this plant yielded seeds with the same biochemical features, indicating that the new phenotype is of mutational origin.
Maillot-Vernier
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P
UA 1182, Laboratoire de Biologie et Biochimie du Développement des Plantes, Institut de Botanique, Strasbourg Cedex, France.
Schaller
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H
Benveniste
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P
Belliard
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G
D002849:Chromatography, Gas; D065607:Cytochrome P-450 Enzyme Inhibitors; D004351:Drug Resistance / Q000235:genetics; D005838:Genotype; D010088:Oxidoreductases / Q000037:antagonists & inhibitors*; D010944:Plants / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D010947:Plants, Toxic; D058886:Sterol 14-Demethylase; D013261:Sterols / Q000096:biosynthesis* / Q000302:isolation & purification; D014026:Nicotiana / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D014230:Triazoles / Q000494:pharmacology*
D065607:Cytochrome P-450 Enzyme Inhibitors; D013261:Sterols; D014230:Triazoles; C061986:LAB 170250F; D010088:Oxidoreductases; D058886:Sterol 14-Demethylase