title: Effects of rat ovariectomy on CSF monoamine metabolite levels and elimination.
abstract: Cerebrospinal fluid (CSF) was removed from the third ventricle of anesthetized male, female, and ovariectomized rats. CSF 3,4-dihydroxyphenylethylamine and serotonin metabolite levels [dihydroxyphenylacetic acid, homovanillic acid (HVA), and 5-hydroxyindoleacetic acid (5-HIAA)] were determined on 15-min samples by liquid chromatography coupled with electrochemical detection. Monoamine oxidase inhibition was used for studying metabolite turnover in the CSF. No difference was observed between male, ovariectomized, and sham-operated female rats. However, ventricular CSF HVA and 5-HIAA levels were significantly higher in the ovariectomized than in the sham-operated rats. These differences do not reflect effects of ovariectomy on brain metabolite production but indicate slower metabolite elimination from the CSF.
authors.forename: C
authors.initials: C
authors.lastname: Duval
authors.forename: E
authors.initials: E
authors.lastname: Mignot
mesh_terms: D015102:3,4-Dihydroxyphenylacetic Acid / Q000134:cerebrospinal fluid*; D000818:Animals; D002369:Castration; D005260:Female; D006719:Homovanillic Acid / Q000134:cerebrospinal fluid*; D006897:Hydroxyindoleacetic Acid / Q000134:cerebrospinal fluid*; D008297:Male; D008996:Monoamine Oxidase Inhibitors; D010293:Pargyline / Q000494:pharmacology; D010648:Phenylacetates / Q000134:cerebrospinal fluid*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D008996:Monoamine Oxidase Inhibitors; D010648:Phenylacetates; D015102:3,4-Dihydroxyphenylacetic Acid; D006897:Hydroxyindoleacetic Acid; D010293:Pargyline; D006719:Homovanillic Acid