[Effect of hemodialysate and its peptide fragments on stromal cells and heme synthesis in bone marrow culture and the activity of selected enzymes and GSH in human erythrocytes. II. Effect on heme synthesis by the erythroid cells of the human bone marrow in vitro].
Effect hemodialysate or its three peptide fractions on the heme synthesis in in vitro cultured human bone marrow erythroid cells has been studied using the 59Fe incorporation method. Heme has been extracted from human bone marrow erythroblasts. Under the hemodialysate (concentrations equal to 350 and 200 micrograms/ml) and the fraction II (concentrations equal to 14 micrograms/ml of medium calculated against the protein content) effect the 59Fe incorporation (cpm) 10(6) of nucleated marrow cells) has statistically been elevated. Hemodialysate showing capability to stimulate the heme synthesis contained peptides having molecular weights in the range of 5 to 45 kda; the molecular weights of fraction II components remained in the range of 5 to 15 kda. Since other hemodialysate peptide fractions showed no stimulatory effect on the 59Fe incorporation to heme it could be suggested that the active substance has molecular weight corresponding to 5 to 15 kda. Moreover, the substances correspond not to erythropoietin which molecular weight remains in the range of 34 to 40 kda. On the whole, it has been demonstrated that the blood factor stimulating heme synthesis reaches the dialysate.
Smoleński
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D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003470:Culture Media; D015314:Dialysis Solutions / Q000494:pharmacology*; D004912:Erythrocytes / Q000378:metabolism*; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006418:Heme / Q000096:biosynthesis*; D015312:Hemodialysis Solutions / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D010446:Peptide Fragments / Q000494:pharmacology*
D003470:Culture Media; D015314:Dialysis Solutions; D015312:Hemodialysis Solutions; D010446:Peptide Fragments; D006418:Heme
Wpływ hemodializatu i jego frakcji peptydowych na komórki podścieliska i synteze hemu w hodowli szpiku oraz na aktywność wybranych enzymów i stezenie GSH w krwinkach czerwonych człowieka. Cześć II: Wpływ na synteze hemu przez komórki erytroidalne szpiku ludzkiego in vitro.