The selection of antibodies with defined desorption properties from precipitated immune complexes for use in immunoadsorption procedures.
A general method for preparing immunosorbents with preselected antibody avidity is described. The method, which is a modification of a method described previously, also includes immunospecific purification of the ligand prior to coupling on the gel matrix. Polyclonal anti-alpha-1-fetoprotein antibodies in precipitated immune complexes were separated according to their avidity (low, intermediate and high) by dissociation with agents of increasing efficiency. After solid-phase coupling the antigen binding activity of the separated antibody preparations was examined according to recovery, capacity and binding strength. Antibodies of intermediate avidity derived from the immune complexes demonstrated optimal properties for preparative affinity chromatography.
Folkersen
J
J
Teisner
B
B
Westergaard
J G
JG
Grudzinskas
J G
JG
D000915:Antibody Affinity; D000936:Antigen-Antibody Complex; D002846:Chromatography, Affinity / Q000379:methods; D007163:Immunosorbent Techniques; D000509:alpha-Fetoproteins / Q000276:immunology / Q000302:isolation & purification*
D000936:Antigen-Antibody Complex; D000509:alpha-Fetoproteins