Concentrations of mRNAs encoding for preprosomatostatin and preprocholecystokinin are increased after kainic acid-induced seizures.
Concentrations of preprosomatostatin-mRNA and preprocholecystokinin-mRNA were determined by Northern blot analysis in rats 2, 10, and 30 days after strong seizures induced by a single intraperitoneal injection of kainic acid. At all time intervals examined, levels of preprosomatostatin-mRNA were increased in the frontal cortex; so were levels of preprocholecystokinin-mRNA in the striatum. Transient increases, i.e., 2 days after kainic acid, of preprocholecystokinin-mRNA were observed in the frontal cortex and the substantia nigra. Preprocholecystokinin-mRNA was reduced in the hippocampus 2 and 10 days after kainic acid. Both preprosomatostatin- and preprocholecystokinin-mRNA levels showed a tendency to be reduced in the amygdala/pyriform cortex at all three time intervals. The increases in mRNA levels suggest enhanced rates of synthesis of the respective neuropeptides subsequent to kainic acid-induced seizures. They may also reflect a prolonged increase in the activity of the respective peptide-containing neurons. This is of special interest in the frontal cortex, since in this area both neuropeptides are found in interneurons and are widely colocalized with gamma-aminobutyric acid.
Olenik
C
C
Department of Pharmacology, University of Freiburg, Republic of Germany.
Meyer
D K
DK
Marksteiner
J
J
Sperk
G
G
D000818:Animals; D002766:Cholecystokinin / Q000378:metabolism*; D007608:Kainic Acid; D008297:Male; D011498:Protein Precursors / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism*; D013004:Somatostatin / Q000378:metabolism*
D011498:Protein Precursors; D012333:RNA, Messenger; D013004:Somatostatin; C042082:preprocholecystokinin; D002766:Cholecystokinin; D007608:Kainic Acid