1,25-dihydroxyvitamin D-responsive element and glucocorticoid repression in the osteocalcin gene.
The active hormonal form of vitamin D3, 1,25-dihydroxyvitamin D3[1,25(OH), which regulates cellular replication and function in many tissues and has a role in bone and calcium homeostasis, acts through a hormone receptor homologous with other steroid and thyroid hormone receptors. A 1,25(OH)2D3-responsive element (VDRE), which is within the promoter for osteocalcin [a bone protein induced by 1,25(OH)2D3] is unresponsive to other steroid hormones, can function in a heterologous promoter, and contains a doubly palindromic DNA sequence (TTGGTGACTCACCGGGTGAAC; -513 to -493 bp), with nucleotide sequence homology to other hormone responsive elements. The potent glucocorticoid repression of 1,25(OH)2D3 induction and of basal activity of this promoter acts through a region between -196 and +34 bp, distinct from the VDRE.
Morrison
N A
NA
Garvan Institute of Medical Research, St. Vincents Hospital, Sydney, Australia.
Shine
J
J
Fragonas
J C
JC
Verkest
V
V
McMenemy
M L
ML
Eisman
J A
JA
D000818:Animals; D001483:Base Sequence; D002117:Calcitriol / Q000494:pharmacology*; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D004247:DNA / Q000235:genetics*; D003907:Dexamethasone / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects*; D005938:Glucocorticoids / Q000494:pharmacology*; D006801:Humans; D008969:Molecular Sequence Data; D015675:Osteocalcin / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D051381:Rats; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection; D014407:Tumor Cells, Cultured
D005938:Glucocorticoids; D015675:Osteocalcin; D003907:Dexamethasone; D004247:DNA; D015500:Chloramphenicol O-Acetyltransferase; D002117:Calcitriol