Rotary-shadowed freeze-fracture replicas: a simple method for the analysis of fracture faces.
In rotary-shadowed freeze-fracture replicas, intramembrane particles on the periphery of a membrane fracture face are not uniformly shadowed from all sides. Those eccentrically positioned intramembrane particles with a net centripetally directed shadowing are on a convex fracture face. In contrast, those eccentrically positioned intramembrane particles with a net centrifugally directed shadowing are on a concave fracture face. Centrally positioned intramembrane particles on convex faces are uniformly shadowed from all sides; however, central depressions of concave faces are often unshadowed.
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D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D006224:Cricetinae; D005614:Freeze Fracturing / Q000379:methods*; D008297:Male; D008647:Mesocricetus; D010984:Platinum; D013194:Staining and Labeling; D014132:Trachea / Q000648:ultrastructure
D010984:Platinum