Evidence that autoreactive T hybridomas recognize multiple epitopes on the I-Ak molecule.
In this study we attempt to define the number of determinants on the I-Ak molecule recognized by a panel of autoreactive I-Ak-specific T cell hybridomas. The recognition sites or histotopes of a panel of autoreactive I-Ak-restricted T cell hybridomas was determined in two ways: 1) by their ability to be activated by a panel of A betak and A alphak mutant antigen-presenting cell lines, and 2) by inhibition of activation by anti-I-Ak monoclonal antibodies. The results are most consistent with the presence of multiple distinct recognition sites on the I-Ak molecule. However, an alternative explanation, that the T cell hybridomas recognize the same self epitope on Class II molecules, but with different affinity, cannot be excluded.
Glimcher
L H
LH
Serog
B
B
McKean
D J
DJ
Beck
B N
BN
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology; D000938:Antigen-Presenting Cells / Q000276:immunology; D001667:Binding, Competitive; D002460:Cell Line; D003470:Culture Media; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D005312:Fetal Blood / Q000502:physiology; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology; D064532:Histocompatibility Antigen H-2D; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008814:Mice, Inbred NZB; D009154:Mutation; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D003470:Culture Media; D000939:Epitopes; D006183:H-2 Antigens; D064532:Histocompatibility Antigen H-2D; D000949:Histocompatibility Antigens Class II