Peroxisomal oxidases: cytochemical localization and biological relevance.
(1) alpha-HAOX has a broad substrate specificity. In rat kidney, the enzyme reacts with aliphatic and aromatic alpha-hydroxy acids, in rat liver, however, only with aliphatic ones. (2) The best substrate for the demonstration of alpha-HAOX activity in rat and human liver is glycolate. (3) alpha-hydroxy butyric acid is the best substrate in the luminometric assay for the demonstration of alpha-HAOX activity in the rat kidney, whereas glycolate is not catalysed by the enzyme. (4) In the proximal tubulus epithelial cells of the rat kidney alpha-HAOX is concentrated in the peripheral matrix of the peroxisomes.
Angermüller
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Department of Anatomy and Cell Biology, University of Heidelberg.
D000429:Alcohol Oxidoreductases / Q000032:analysis; D000818:Animals; D003605:D-Amino-Acid Oxidase / Q000032:analysis; D006651:Histocytochemistry; D006801:Humans; D050356:Lipid Metabolism; D008830:Microbodies / Q000201:enzymology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D010088:Oxidoreductases / Q000032:analysis*; D014503:Urate Oxidase / Q000032:analysis; D014969:Xanthine Oxidase / Q000032:analysis
D010088:Oxidoreductases; D000429:Alcohol Oxidoreductases; C021380:L-2-hydroxyacid oxidase; D014969:Xanthine Oxidase; D003605:D-Amino-Acid Oxidase; D014503:Urate Oxidase