title: Liver microsomal delta 6 and delta 5 linoleic acid desaturation in female BB rats.
abstract: Rat liver microsomal delta 6 and delta 5 desaturation are defective in experimental diabetes, but this defect is correctable with insulin treatment. Rat liver fatty acid composition and delta 6 and delta 5 desaturation were studied in the spontaneously diabetic adult female Bio-Breeding (BB) rat. Control Wistar rats and BB rats (4 weeks of diabetes), that received insulin (1 IU.100 g body weight-1.day-1), were killed 20 h after the last insulin injection. delta 6 and delta 5 desaturase activities were estimated from the incubation of liver microsomes with (1-14C) 18:2, n-6 or (2-14C) 20:3, n-6, respectively, and the fatty acid composition of the liver and microsomal liver lipids were investigated. Under experimental conditions delta 6 and delta 5 desaturase activities were unchanged in the BB rats when compared to the control rats. Impairment of the liver fatty acid composition of diabetic BB rats is not consistent with normal desaturase activity and may be explained by factors other than desaturation disturbance.
authors.affiliation: Laboratoire de Physiologie Animale et de la Nutrition, Faculté des Sciences Mirande, Dijon, France.
authors.forename: B
authors.initials: B
authors.lastname: Chanussot
authors.forename: M
authors.initials: M
authors.lastname: Narce
authors.forename: J P
authors.initials: JP
authors.lastname: Poisson
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D000090943:Delta-5 Fatty Acid Desaturase; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D044943:Fatty Acid Desaturases / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism*; D050602:Linoleoyl-CoA Desaturase; D008055:Lipids / Q000032:analysis*; D008099:Liver / Q000032:analysis / Q000033:anatomy & histology; D008862:Microsomes, Liver / Q000201:enzymology*; D009929:Organ Size; D010714:Phosphatidylethanolamines / Q000032:analysis; D051381:Rats; D011913:Rats, Inbred BB; D011919:Rats, Inbred Strains; D014280:Triglycerides / Q000032:analysis
chemical_list: D001786:Blood Glucose; D000090943:Delta-5 Fatty Acid Desaturase; D005227:Fatty Acids; D008041:Linoleic Acids; D008055:Lipids; D010714:Phosphatidylethanolamines; D014280:Triglycerides; D019787:Linoleic Acid; D044943:Fatty Acid Desaturases; D050602:Linoleoyl-CoA Desaturase