title: [Isolation of alpha-latrotoxin from karakurt spider venom using monoclonal antibodies].
abstract: A method of alpha-latrotoxin (LT) isolation from the venom of Latrodectus mactans tredecimguttatus by means of immunoaffinity chromatography on sepharose conjugated with monoclonal antibodies against LT has been developed. This one-step, high-yield, relatively simple and rapid procedure yields active LT for structural and functional studies of its receptor.
authors.forename: V N
authors.initials: VN
authors.lastname: Pashkov
authors.forename: G I
authors.initials: GI
authors.lastname: Kovalevskaia
authors.forename: V G
authors.initials: VG
authors.lastname: Krasnoperov
authors.forename: O V
authors.initials: OV
authors.lastname: Bulgakov
mesh_terms: D000911:Antibodies, Monoclonal; D001180:Arthropod Venoms / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D013111:Spider Venoms / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000911:Antibodies, Monoclonal; D001180:Arthropod Venoms; D013111:Spider Venoms; C020763:alpha-latrotoxin
vernacular_title: Vydelenie alpha-latrotoksina iz iada pauka karakurta s pomoshchiu monoklonalnykh antitel.