A modified method for the production of 36ClO3- for use in plant nitrate transport studies.
A modified method for the production and purification of 36ClO3- in high yield is described. This procedure, involving the electrolytic oxidation of 36Cl- in a cell with simple electrode design and purification of the electrolysis products (36Cl-, 36ClO3-, and 36ClO4-) by aqueous column chromatography, allows for the recovery of about 80% of the initial radiolabel as 36ClO3-. The method is rapid and suitable for the production of this radiolabeled anion for use as a tracer analog for nitrate in plant membrane transport experiments.
Ruiz-Cristin
J
J
Department of Agronomy, University of Illinois, Urbana 61801.
Chodera
A J
AJ
Briskin
D P
DP
D001692:Biological Transport; D002704:Chlorates / Q000378:metabolism*; D002713:Chlorine; D002850:Chromatography, Gel; D004572:Electrolysis; D007553:Isotope Labeling / Q000379:methods; D009566:Nitrates / Q000493:pharmacokinetics*; D010084:Oxidation-Reduction; D010944:Plants / Q000378:metabolism*; D011868:Radioisotopes
D002704:Chlorates; D009566:Nitrates; D011868:Radioisotopes; D002713:Chlorine