Effect of ionising radiation on the axon reaction of mouse anterior horn motor neurons. A histological and immunocytochemical study using a monoclonal antibody to neurofilament protein.
The changes taking place in irradiated central nervous tissue prior to the onset of delayed radionecrosis are poorly understood, but functional abnormalities occurring during the latent interval after irradiation are likely to be of importance. In order to investigate functional disturbances in neurones during this period, unilateral sciatic nerve crush was performed in mice following sub-lethal X-irradiation of the lumbar spinal cord. Alterations in the axon reaction of anterior horn cells were studied using a monoclonal antibody to neurofilament protein. With irradiation immediately prior to crush, the normal, well-defined increase in perikaryal neurofilament protein was significantly diminished, although there was no concurrent radiation necrosis and no alterations were seen in contralateral neurones with intact distal axon processes. The effect was more marked in neurones irradiated one month prior to nerve crush, and in the non-irradiated nerve crush region regeneration was delayed, with diminished neurofilament protein in the regenerating axons. These observations indicate that ionising radiation can progressively impair the ability of neurones to synthesise neurofilament protein during distal axon regeneration. This may result from inadequate repair of radiation induced DNA strand-breaks, but may also follow more generalised damage to protein transcription enzymes and RNA metabolism.
Moss
T H
TH
Lewkowicz
S J
SJ
D000818:Animals; D000870:Anterior Horn Cells / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D001369:Axons / Q000528:radiation effects; D004247:DNA / Q000378:metabolism; D007124:Immunoenzyme Techniques; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D009046:Motor Neurons / Q000528:radiation effects*; D009416:Nerve Regeneration / Q000528:radiation effects*; D016900:Neurofilament Proteins; D012313:RNA / Q000378:metabolism; D013127:Spinal Nerves / Q000293:injuries* / Q000378:metabolism
D007381:Intermediate Filament Proteins; D016900:Neurofilament Proteins; D012313:RNA; D004247:DNA