title: Cytologic detection of Cryptococcus neoformans in cerebrospinal fluid. Rapid screening methods.
abstract: Yeasts of Cryptococcus neoformans in cerebrospinal fluid (CSF) were studied using polarized light and fluorescence microscopy. Ultraviolet-induced fluorescence of wet-fixed, Papanicolaou-stained smears and polarization of air-dried, Diff-Quik or Leishman-stained preparations are complementary methods that allow for rapid screening for and immediate identification of Cryptococcus in cytologic preparations of CSF.
authors.forename: R W
authors.initials: RW
authors.lastname: Brown
authors.forename: R J
authors.initials: RJ
authors.lastname: Clarke
authors.forename: M F
authors.initials: MF
authors.lastname: Gonzales
mesh_terms: D001718:Birefringence; D002555:Cerebrospinal Fluid / Q000382:microbiology*; D003453:Cryptococcosis / Q000134:cerebrospinal fluid* / Q000382:microbiology; D003454:Cryptococcus / Q000166:cytology*; D003455:Cryptococcus neoformans / Q000166:cytology*; D004801:Eosine Yellowish-(YS); D006801:Humans; D008581:Meningitis / Q000134:cerebrospinal fluid* / Q000382:microbiology; D008856:Microscopy, Fluorescence; D008859:Microscopy, Polarization; D013194:Staining and Labeling
chemical_list: D004801:Eosine Yellowish-(YS)