Quantitation by computerized visual image analysis of gastric mucosal lesions induced in mice and rats by non-steroidal anti-inflammatory drugs.
A method is described for the quantative determination of gastric mucosal lesions induced by non-steroidal anti-inflammatory drugs in mice and rats. The area and number of gastric lesions present in formalin-fixed, glycerol-cleared mucosa is determined by computerized visual image analysis using instrumentation as described. The method is also applied to the determination of the protective effects of anti-ulcer agents (e.g. prostaglandin E2, pirenzepine). Thus this method affords unambiguous sensitive and determination of the percentage area of the mucosa damaged and the number of the lesions.
Rainsford
K D
KD
Department of Biomedical Sciences, McMaster University, Health Sciences Faculty, Hamilton, Ontario, Canada.
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000633:toxicity*; D018723:Bethanechol; D001626:Bethanechol Compounds / Q000494:pharmacology; D015232:Dinoprostone / Q000494:pharmacology; D004357:Drug Synergism; D005753:Gastric Mucosa / Q000187:drug effects*; D007091:Image Processing, Computer-Assisted; D051379:Mice; D010438:Peptic Ulcer Hemorrhage / Q000139:chemically induced / Q000473:pathology*; D010890:Pirenzepine / Q000494:pharmacology; D051381:Rats; D012110:Reserpine / Q000494:pharmacology; D013276:Stomach Ulcer / Q000139:chemically induced / Q000150:complications / Q000473:pathology*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D001626:Bethanechol Compounds; D018723:Bethanechol; D010890:Pirenzepine; D012110:Reserpine; D015232:Dinoprostone