Increased sensitivity of the fluorescent treponemal antibody absorption test with biotin/avidin: a comparison with conventional FTA-ABS.
The biotin/avidin system was adapted to the FTA technique for detection of IgG, IgM, and IgA antibodies specific to Treponema pallidum. By using biotin/avidin IgG antibodies could be detected in a serum dilution 15 000 times higher, and IgM 3 000 times higher than was possible with conventional FTA technique. IgA antibodies, however, were not detected with any of the methods.
Berntsson
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D000293:Adolescent; D000328:Adult; D001360:Avidin; D001710:Biotin; D002648:Child; D002675:Child, Preschool; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007223:Infant; D008297:Male; D008875:Middle Aged; D013587:Syphilis / Q000276:immunology; D013588:Syphilis Serodiagnosis / Q000379:methods*; D014210:Treponema pallidum / Q000276:immunology*
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D001360:Avidin; D001710:Biotin