title: Monoclonal antibodies to cytochrome P-450 immunopurify a 45-kDa protein from a human lymphoblastoid cell line.
abstract: Monoclonal antibodies (MAbs) to rat liver cytochromes P-450 have previously been used for successful immunopurification of cytochromes P-450 from animal tissues. We now report application of this MAb-based immunopurification technique to the human lymphoblastoid AHH-1 cell line. Immunopurification carried out with 3 different MAbs each yielded a 45-kDa polypeptide. The purified protein contains an MAb-specific epitope present on cytochromes P-450, and may therefore be a human cytochrome P-450.
authors.forename: F K
authors.initials: FK
authors.lastname: Friedman
authors.forename: J V
authors.initials: JV
authors.lastname: Pastewka
authors.forename: R C
authors.initials: RC
authors.lastname: Robinson
authors.forename: S S
authors.initials: SS
authors.lastname: Park
authors.forename: M A
authors.initials: MA
authors.lastname: Marletta
authors.forename: H V
authors.initials: HV
authors.lastname: Gelboin
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D002460:Cell Line; D003577:Cytochrome P-450 Enzyme System / Q000276:immunology / Q000302:isolation & purification*; D004790:Enzyme Induction / Q000187:drug effects; D000939:Epitopes / Q000276:immunology; D006801:Humans; D007158:Immunologic Techniques; D008099:Liver / Q000201:enzymology; D008214:Lymphocytes / Q000201:enzymology*; D008748:Methylcholanthrene / Q000494:pharmacology; D010634:Phenobarbital / Q000494:pharmacology; D051381:Rats
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D008748:Methylcholanthrene; D003577:Cytochrome P-450 Enzyme System; D010634:Phenobarbital