title: Regulation of mouse ornithine decarboxylase activity by cell growth, serum and tetradecanoyl phorbol acetate is governed primarily by sequences within the coding region of the gene.
abstract: To determine the genetic elements required for modulation of ornithine decarboxylase (ODC) activity in response to cell growth or treatment with serum or with tetradecanoyl phorbol acetate, ODC-deficient cells were transfected with a series of recombinant DNAs encoding mouse ODC. All of the transfected cells expressing an intact mouse ODC protein displayed regulation of ODC activity, including those expressing a construct deprived of all ODC-specific sequence information except the protein-coding region. ODC mRNA changed much less than enzymatic activity. A mutation of the protein-coding region that converted ODC from an unstable to a stable intracellular protein attenuated the regulatory response. We conclude that post-transcriptional events associated with ODC degradation dominate the response to these stimuli.
authors.affiliation: Department of Microbiology and Immunology, University of California, San Francisco 94143.
authors.forename: T
authors.initials: T
authors.lastname: van Daalen Wetters
authors.forename: M
authors.initials: M
authors.lastname: Brabant
authors.forename: P
authors.initials: P
authors.lastname: Coffino
mesh_terms: D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured; D003470:Culture Media; D004274:DNA, Recombinant / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005796:Genes / Q000187:drug effects; D007700:Kinetics; D008223:Lymphoma; D051379:Mice; D009955:Ornithine Decarboxylase / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014162:Transfection
chemical_list: D003470:Culture Media; D004274:DNA, Recombinant; D009955:Ornithine Decarboxylase; D013755:Tetradecanoylphorbol Acetate