Comparison of RNA from healthy and scrapie-infected hamster brain.
Density gradient fractions prepared from healthy or scrapie-infected hamster brain tissue enriched in plasma membrane vesicles were treated with nucleases prior to phenol extraction and ethanol precipitation. The recovered nucleic acids were 3' end-labelled and run on one-dimensional polyacrylamide gels. Autoradiography revealed the presence of low molecular weight RNAs (4S) in both healthy and scrapie samples. Two-dimensional fingerprint analysis indicated that the RNAs isolated from scrapie-infected hamsters contained oligonucleotides that were not present in RNAs isolated from healthy hamsters.
German
T L
TL
McMillan
B C
BC
Castle
B E
BE
Dees
C
C
Wade
W F
WF
Marsh
R F
RF
D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry; D006224:Cricetinae; D012313:RNA / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis*; D012608:Scrapie / Q000378:metabolism*; D012756:Sheep; D014779:Virus Replication
D012367:RNA, Viral; D012313:RNA