title: Isolation and characterization of a cDNA clone encoding human aromatic L-amino acid decarboxylase.
abstract: The nucleotide sequence of a cDNA clone that includes the entire coding region of human aromatic L-amino acid decarboxylase gene is presented. A human pheochromocytoma cDNA library was screened using an oligonucleotide probe which corresponded to a partial amino acid sequence of the enzyme purified from the human pheochromocytoma. The isolated cDNA clone encoded a protein of 480 amino acids with a calculated molecular mass of 53.9 kDa. The amino acid sequence Asn-Phe-Asn-Pro-His-Lys-Trp around a possible cofactor (pyridoxal phosphate) binding site is identical in human, Drosophila, and pig enzymes.
authors.affiliation: Department of Biochemistry, Nagoya University School of Medicine, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ichinose
authors.forename: Y
authors.initials: Y
authors.lastname: Kurosawa
authors.forename: K
authors.initials: K
authors.lastname: Titani
authors.forename: K
authors.initials: K
authors.lastname: Fujita
authors.forename: T
authors.initials: T
authors.lastname: Nagatsu
mesh_terms: D000310:Adrenal Gland Neoplasms / Q000201:enzymology* / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000235:genetics* / Q000302:isolation & purification; D004330:Drosophila / Q000201:enzymology / Q000235:genetics; D015723:Gene Library; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010673:Pheochromocytoma / Q000201:enzymology* / Q000235:genetics; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004273:DNA, Neoplasm; D001142:Aromatic-L-Amino-Acid Decarboxylases