title: Gene rearrangement in cells with natural killer activity and expression of the beta-chain of the T-cell antigen receptor.
abstract: The mammalian host defence system can be divided broadly into adaptive and non-adaptive immunity. Adaptive immunity is acquired and is mediated by B and T lymphocytes. Non-adaptive immunity is mediated in part by a small subclass of heterogeneous peripheral blood mononuclear cells. This population, termed null cells, consists of haematopoietic precursors and cells mediating natural killer (NK) activity and antibody-dependent cellular cytotoxicity (ADCC). NK cells are a class of non-adherent, non-phagocytic, rapidly cytotoxic lymphocytes which can efficiently lyse a wide variety of tumour cells, virally infected cells and immature cell types of normal origin. Despite the broad range of targets, only a limited number of specificities are thought to be involved in target-cell recognition. Morphologically, NK cells are large granular lymphocytes, but they have been shown to exhibit cell-surface markers characteristic of both T cells and monocytes, raising doubt over their lineage. The recent cloning of the beta-chain of the T-cell antigen receptor has now allowed us to investigate whether some NK cells are T-cell-related. We have examined rearrangement and expression of the beta-chain of the T-cell receptor in cloned murine NK cell lines and fresh murine NK cell populations, and our results support the hypothesis that a subpopulation of NK cells is related to T cells and provide basis for examining whether some NK activity is mediated by a small number of T-cell receptors.
authors.forename: Y
authors.initials: Y
authors.lastname: Yanagi
authors.forename: N
authors.initials: N
authors.lastname: Caccia
authors.forename: M
authors.initials: M
authors.lastname: Kronenberg
authors.forename: B
authors.initials: B
authors.lastname: Chin
authors.forename: J
authors.initials: J
authors.lastname: Roder
authors.forename: D
authors.initials: D
authors.lastname: Rohel
authors.forename: T
authors.initials: T
authors.lastname: Kiyohara
authors.forename: R
authors.initials: R
authors.lastname: Lauzon
authors.forename: B
authors.initials: B
authors.lastname: Toyonaga
authors.forename: K
authors.initials: K
authors.lastname: Rosenthal
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004262:DNA Restriction Enzymes; D005786:Gene Expression Regulation; D005796:Genes; D007694:Killer Cells, Natural / Q000502:physiology*; D046911:Macromolecular Substances; D012313:RNA / Q000235:genetics; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D046911:Macromolecular Substances; D011948:Receptors, Antigen, T-Cell; D012313:RNA; D004262:DNA Restriction Enzymes