An improved pyrogallol red-molybdate method for determining total urinary protein.
We adapted the pyrogallol red-molybdate method for total urinary protein to the Cobas Bio centrifugal analyzer. The method is simple, rapid, sensitive, and inexpensive. Addition of 25 mg of sodium dodecyl sulfate per liter to the reagent modifies protein reactivities so that the chromogenicity of human gamma globulins is the same as that of albumin. Results by this method and a comparison method that included gel filtration and a modified biuret reaction correlated well (r = 0.951).
Orsonneau
J L
JL
Laboratoire de Chimie A--C.H.R. Hôtel-Dieu, Nántes, France.
Douet
P
P
Massoubre
C
C
Lustenberger
P
P
Bernard
S
S
D000328:Adult; D001322:Autoanalysis; D004396:Coloring Agents; D006801:Humans; D008982:Molybdenum; D011507:Proteinuria / Q000652:urine*; D011748:Pyrogallol / Q000031:analogs & derivatives; D011786:Quality Control; D012016:Reference Values; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology
D004396:Coloring Agents; D011748:Pyrogallol; C044659:molybdate; C058731:pyrogallol sulfonphthalein; D012967:Sodium Dodecyl Sulfate; D008982:Molybdenum