Preparation of a new antivenin by affinity chromatography.
Polyacrylamide gel affinity chromatography was employed to isolate and purify antibodies to the antigens of the venoms of four rattlesnakes. The antivenins were studied for their neutralizing properties on a number of pharmacologic preparations. It was found that the purified antibodies (IgG) were more efficacious than the commercially prepared antivenin in neutralizing the lethal, cytolytic, hemorrhagic, platelet aggregating, and other deleterious effects of the venoms. In addition, the purified antibodies gave no evidence of producing anaphylaxis or anaphylactoid reactions in animals sensitized to horse serum. The proposed technique is also more simple than current production methods, is time-saving, and less expensive.
Russell
F E
FE
Sullivan
J B
JB
Egen
N B
NB
Jeter
W S
WS
Markland
F S
FS
Wingert
W A
WA
Bar-Or
D
D
D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000302:isolation & purification; D000997:Antivenins / Q000276:immunology / Q000302:isolation & purification*; D001777:Blood Coagulation / Q000187:drug effects; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003435:Crotalid Venoms / Q000276:immunology / Q000494:pharmacology; D005260:Female; D005340:Fibrinogen / Q000037:antagonists & inhibitors; D006168:Guinea Pigs; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D051379:Mice; D009469:Neuromuscular Junction / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects; D012911:Snakes
D000906:Antibodies; D000997:Antivenins; D003435:Crotalid Venoms; D005340:Fibrinogen