[Heat sensitivity of human cancer cells and abnormal expression of heat shock protein 70].
We investigated the heat-sensitivities of human normal and cancer cells at different growth conditions in vitro. We found no difference in sensitivities between normal and cancer cells at growing condition. Normal cells at confluence, however, reduced their heat-sensitivity 5-6 times than at growing condition, while the cancer cells did not. Analysis by monoclonal antibodies for hsp 70 showed the differential staining patterns between normal and cancer cells after treatment with heat (43 degrees C for 2 hours). The constitutive expression levels of hsp 70 in cancer cells were 2-3 times higher than those in normal cells. However, the degrees of hsp 70 induction by heat shock treatment in cancer cells were lower than those in normal cells. These results suggest that cancer cells may be abnormal in expression mechanisms of hsp 70.
Watanabe
M
M
RI Research Center, School of Med., Yokohama City Univ.
Suzuki
K
K
D002455:Cell Division; D002470:Cell Survival / Q000502:physiology; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006358:Hot Temperature; D006801:Humans; D014407:Tumor Cells, Cultured / Q000378:metabolism* / Q000473:pathology
D006360:Heat-Shock Proteins