Studies of streptococcal membrane antigen--binding cells in acute rheumatic fever.
Blood specimens from patients with rheumatic heart disease in both India and New Mexico were typed for the presence of B cell alloantigen 883 by use of a mouse monoclonal antibody with identical specificity to the original 883 human alloantiserum. Strong relative segregation was recorded for 883 positive B cell typing in patients with rheumatic heart disease in both geographic locations as compared with that in normal unaffected controls. In patients with acute rheumatic fever, studies of actual B-lymphocyte membrane binding by anti-883 monoclonal antibody and sonicated group A streptococcal membrane antigens showed separate but contiguous localization on isolated cell surfaces. Although physically distinct, 883 B cell alloantigen and sonicated group A streptococcal membrane antigens moved together in cell capping studies after incubation at 37 degrees C. These findings reaffirm the apparent close association between 883 B cell alloantigen and rheumatic heart disease. They also demonstrate that the B cell alloantigen 883 itself is physically distinct from but very close to sites on antigen-reactive B cells actually binding to group A streptococcal membrane antigens.
Williams
R C
RC
Raizada
V
V
Prakash
K
K
Sharma
K B
KB
Anand
I
I
Ganguly
N K
NK
Zabriskie
J B
JB
D000208:Acute Disease; D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology; D000939:Epitopes; D006801:Humans; D007519:Isoantigens / Q000032:analysis; D051379:Mice; D011947:Receptors, Antigen, B-Cell / Q000032:analysis*; D012213:Rheumatic Fever / Q000097:blood / Q000473:pathology*; D013291:Streptococcus / Q000276:immunology*; D013297:Streptococcus pyogenes / Q000276:immunology
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D000939:Epitopes; D007519:Isoantigens; D011947:Receptors, Antigen, B-Cell