title: Synthesis of 20 alpha-hydroxylated steroids by ovaries of the dab (Limanda limanda).
abstract: Dab (Limanda limanda) ovarian fragments were incubated in vitro with either [4,7-3H]pregnenolone or 17 alpha-hydroxy[1,2,6,7-3H]progesterone to investigate the pattern of steroidogenesis. A major enzyme found in the dab ovary was 20 alpha-hydroxysteroid dehydrogenase. Among the steroids that were tentatively identified in ovarian incubates were 17 alpha,20 alpha-dihydroxy-4-pregnen-3-one (17,20 alpha-P). 17 alpha,20 alpha-dihydroxy-5 beta-pregnan-3-one, 3 beta, 17 alpha,20 alpha-trihydroxy-5 beta-pregnane (3 beta,17,20 alpha-P-5 beta), and 3 alpha,17 alpha,20 alpha-trihydroxy-5 beta-pregnane. The presence of these steroids in plasma of mature female and male dabs was studied by radioimmunoassay. The antiserum was raised against 17,20 alpha-P. The 17,20 alpha-[3H]P label was produced by incubating place milt with 17 alpha-hydroxy [3H]progesterone. The radioimmunoassay was shown to have a high cross-reaction with the 5 beta-reduced analogues of 17,20 alpha-P and was therefore used, in conjunction with thin-layer chromatography, to measure the steroids. High concentrations of both 17,20 alpha-P and 3 beta,17,20 alpha-P-5 beta found in female and male dab plasma. The possible role of these steroids is discussed.
authors.affiliation: Universidade do Algarve, Faro, Portugal.
authors.forename: A V
authors.initials: AV
authors.lastname: Canario
authors.forename: A P
authors.initials: AP
authors.lastname: Scott
mesh_terms: D019326:17-alpha-Hydroxyprogesterone; D015089:20-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D000818:Animals; D005260:Female; D005399:Fishes / Q000378:metabolism*; D006908:Hydroxyprogesterones / Q000032:analysis / Q000097:blood / Q000378:metabolism*; D008297:Male; D010053:Ovary / Q000032:analysis / Q000378:metabolism*; D011278:Pregnanes / Q000378:metabolism*; D011279:Pregnanetriol / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000378:metabolism; D011280:Pregnanolone / Q000031:analogs & derivatives / Q000032:analysis / Q000097:blood / Q000378:metabolism*; D011284:Pregnenolone / Q000378:metabolism; D011863:Radioimmunoassay
chemical_list: D006908:Hydroxyprogesterones; D011278:Pregnanes; C007433:17,20-dihydroxy-4-pregnen-3-one; D011279:Pregnanetriol; C062103:3,17,20-trihydroxypregnane; C062104:17,20-dihydroxypregnan-3-one; D019326:17-alpha-Hydroxyprogesterone; D011284:Pregnenolone; D011280:Pregnanolone; D015089:20-Hydroxysteroid Dehydrogenases