Quantitative determination of pectic substances as an example of a rhamnopolysaccharide assay.
A quantitative enzymatic assay for rhamnopolysaccharides is described. The procedure is shown with pectic substances as an example. The test is based on the enzymatic degradation of the macromolecules to liberate L-rhamnose. This sugar can be quantitatively determined with the help of L-rhamnose dehydrogenase under concomitant reduction of NAD, thus allowing the quantitative evaluation of the original pectin.
Turecek
P L
PL
Institut für Allgemeine Biochemie der Universität Wien, Austria.
Buxbaum
E
E
Pittner
F
F
D002236:Carbohydrate Conformation; D002237:Carbohydrate Dehydrogenases; D007700:Kinetics; D008969:Molecular Sequence Data; D009243:NAD / Q000032:analysis; D010368:Pectins / Q000032:analysis*; D011134:Polysaccharides / Q000032:analysis*; D012210:Rhamnose / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D011134:Polysaccharides; D009243:NAD; D010368:Pectins; D002237:Carbohydrate Dehydrogenases; C022524:L-rhamnose dehydrogenase; D012210:Rhamnose