Trichinella spiralis: immunization of mice using monoclonal antibody affinity-isolated antigens.
An antigen epitope was identified from the excretory-secretory products of Trichinella spiralis first-stage larvae using monoclonal antibodies, and the glycoprotein antigens bearing this epitope (Ts.49 and Ts.53) were isolated from the crude excretory-secretory preparation by affinity chromatography. In immunization experiments carried out in mice, antigen priming with Ts.49 and Ts.53 resulted in a reduction of muscle larvae resulting from a challenge infection at a level comparable to priming with crude excretory-secretory antigens. In addition, antigens Ts.49 and Ts.53 induced an accelerated expulsion of adult worms from the intestines of immunized mice and reduced the fecundity of remaining female worms.
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D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology / Q000302:isolation & purification; D005260:Female; D007114:Immunization; D007814:Larva; D051379:Mice; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains; D014234:Trichinella / Q000276:immunology*; D014235:Trichinellosis / Q000276:immunology* / Q000517:prevention & control
D000947:Antigens, Helminth; D000939:Epitopes