CD4-, CD8- gamma/delta cells from normal mice respond to a syngeneic B cell lymphoma and can induce its differentiation.
Lymph nodes and spleens from normal unimmunized mice contain small numbers of CD3+, CD4-, CD8- (double negative, DN) T cells. Of these, approximately one-third express the marker Ly-5(B220) in a form previously seen only on normal B cells and a population of DN T cells found in mice genetically prone to develop autoimmunity. DN T cells proliferate when co-cultured with a syngeneic surface Ig+ lymphoma, CH12. After one cycle of stimulation with CH12 almost all of the responding CD3+ DN cells express Ly-5(B220), suggesting that it is an activation marker for some DN T cells. The CH12 responding population also contains cells with two other phenotypes, Thy-1+, CD4-, CD8-, Ly-5(B220)+, sIgM-, CD3- and Thy-1+, CD4+, CD8-, Ly-5(B220)-, sIgM-, CD3+. The Ly-5(B220)+, CD3- population is no longer found after repeated stimulation. While the relationship between these three populations is unknown, DN T cells can proliferate in the absence of CD4+ or CD8+ cells and therefore their proliferation is not dependent on the presence of other T cells or lymphokines produced by CD4+ or CD8+ T cells. Anti-CD3 immunoprecipitation of CH12-responding cells reveals at least seven different receptor proteins of which five can also be precipitated with an anti-(C gamma 1/C gamma 2) monoclonal antibody. Thus at least three different TCR gamma-delta heterodimers are expressed by CH12-responding T cells. The Thy-1+, CD4-, CD8-, Ly-5(B220)+ cells can provide help to CH12 cells for Ig secretion even in the absence of the nominal antigen for the B lymphoma cells.(ABSTRACT TRUNCATED AT 250 WORDS)
Sperling
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Department of Pathology, Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine, Boston, MA 02111.
Wortis
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D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte; D000950:Antigens, Ly; D000954:Antigens, Surface; D015704:CD4 Antigens; D016827:CD8 Antigens; D002454:Cell Differentiation; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D016393:Lymphoma, B-Cell / Q000276:immunology*; D051379:Mice; D008808:Mice, Inbred CBA; D011948:Receptors, Antigen, T-Cell; D016692:Receptors, Antigen, T-Cell, gamma-delta; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D018800:Thy-1 Antigens; D014407:Tumor Cells, Cultured / Q000276:immunology
D000945:Antigens, Differentiation, T-Lymphocyte; D000950:Antigens, Ly; D000954:Antigens, Surface; D015704:CD4 Antigens; D016827:CD8 Antigens; D011948:Receptors, Antigen, T-Cell; D016692:Receptors, Antigen, T-Cell, gamma-delta; D018800:Thy-1 Antigens