Purification of low molecular weight forms of seminal vesicle specific antigen by immunoaffinity chromatography on bound monoclonal antibody MHS-5.
A method has been developed for purification of the low molecular weight forms of seminal vesicle specific antigen (SVSA). Pooled, liquified seminal fluid was fractionated by CM cellulose chromatography followed by two cycles of monoclonal antibody affinity chromatography. Analysis of the final product shows microheterogeneity of the purified immunoreactive peptides in the range of 9-12 kDa. In one run, from 1138 mg starting material, 2.78 mg of SVSA protein was obtained, a recovery of 0.24% of the total protein in the starting material. The purified material as assessed by scanning densitometry of Coomassie stained gels is 99% pure. These findings indicate that the three-step chromatographic method is useful for purifying the low molecular weight forms of SVSA.
Herr
J C
JC
Department of Anatomy and Cell Biology Cancer Center, Medical Center, University of Virginia, Charlottesville 22908.
Conklin
D J
DJ
McGee
R S
RS
D000911:Antibodies, Monoclonal; D000941:Antigens / Q000302:isolation & purification*; D002266:Carboxymethylcellulose Sodium; D002846:Chromatography, Affinity; D006801:Humans; D008297:Male; D008970:Molecular Weight; D011506:Proteins / Q000276:immunology / Q000302:isolation & purification; D012669:Seminal Vesicles / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000941:Antigens; D011506:Proteins; D002266:Carboxymethylcellulose Sodium