Measurement of boar sperm motility by the trans-membrane migration method.
The conventional microscopic methods for evaluating sperm motility of domestic animals are mostly inadequate due to their subjectivity and lack of precision. Recently, a trans-membrane migration method, originally developed for the examination of human sperm motility, has substantially overcome these problems. This study investigated the applicability of the method to boar sperm motility measurement. The apparatus used was simple and consisted only of syringe plungers, poriferous membranes, and modified multi-well culture plates. It measured the proportion of sperm in the semen that moved across the membrane after incubation at 37 degrees C for 3 hr. The sperm motility as measured by this method correlated well with that measured by direct microscopic examinations. The measurement was more reliable using an 8-microns instead of a 5-microns pore-size membrane. The method was found to work equally well for the sperm motility measurement of the semen with a sperm concentration between 1.5 x 10(8)/ml and 6.0 x 10(8)/ml. The results indicate that this method is a simple, objective, quantitative, and reproducible design for the measurement of boar sperm motility.
Lee
S L
SL
Department of Biochemistry, National Yang-Ming Medical College, Taipei, Taiwan, Republic of China.
Sun
C
C
Lin
J H
JH
Hong
C Y
CY
Wei
Y H
YH
D000818:Animals; D002465:Cell Movement; D008297:Male; D008403:Mass Screening / Q000379:methods; D008567:Membranes, Artificial; D013048:Specimen Handling / Q000295:instrumentation / Q000379:methods*; D013081:Sperm Motility
D008567:Membranes, Artificial