In vitro effects of fibrous and nonfibrous silicon nitride on bovine pulmonary macrophages.
Bovine pulmonary macrophages were exposed in vitro to 0.3, 0.1, 0.03, or 0.01 mg/ml of a fibrous silicon nitride, nonfibrous (milled) silicon nitride comminuted from the fibrous powder, alpha-quartz (an active control), or glass beads (an inert control). Functional evaluation of the exposed cells indicated that the fibrous silicon nitride was as cytotoxic as quartz, while the nonfibrous silicon nitride was relatively inert. To further evaluate the mechanisms of cytotoxicity, the cells were exposed to 1 mg/ml of the control and test materials and biochemical studies were performed. Quartz increased release of both the cytoplasmic enzyme, lactate dehydrogenase (LDH), and the lysosomal enzyme, acid phosphatase (AP), consistent with both cell membrane and lysosome lysis. In addition, total protein levels were significantly depressed, suggesting significant impairment of cellular synthetic processes. LDH, but not AP, values were increased with fibrous silicon nitride treatment, but not with the nonfibrous silicon nitride. In contrast to quartz, which increased LDH levels by 65%, the fibrous silicon nitride only increased LDH levels by 11%. Scanning electron micrographs further indicated that the fibrous silicon is cytotoxic and poorly tolerated by macrophages. These studies provide further evidence of morphology as a primary determinant of cytotoxicity since the milled powder test article was comminuted from the fibrous material.
Fisher
G L
GL
Battelle, Columbus, Ohio 43201.
McNeill
K L
KL
Smith
J T
JT
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D004705:Endocytosis / Q000187:drug effects; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D008855:Microscopy, Electron, Scanning; D010587:Phagocytosis / Q000187:drug effects; D011506:Proteins / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D012825:Silicon / Q000494:pharmacology*; D017655:Silicon Compounds; D014343:Trypan Blue / Q000378:metabolism
D011506:Proteins; D017655:Silicon Compounds; D007770:L-Lactate Dehydrogenase; D000135:Acid Phosphatase; D014343:Trypan Blue; C032734:silicon nitride; D012825:Silicon