title: [Experimental demonstration of the annual characteristic of the lines of arrest of skeletal growth in Rana esculenta (Amphibia, anura)].
abstract: Different vital fluorescent labelling (tetracycline, calcein, xylenol orange, alizarine red S) have been injected in a series of young growing green frogs, caught near Paris and kept for 2 years in captivity under conditions quite similar to natural ones. The study of femur and phalanx has been carried out by ground sections observed in UV light and then colored with hematoxyline. The comparison between UV and transmitted light observations shows that the arrested growth lines are annual, laid during winter and never completely destroyed by bone remodelling. Then, they constitute a valuable criterion to assess the age of the individual.
authors.forename: H
authors.initials: H
authors.lastname: Francillon
authors.forename: J
authors.initials: J
authors.lastname: Castanet
mesh_terms: D000365:Age Determination by Skeleton; D000818:Animals; D001846:Bone Development; D001862:Bone Resorption; D001842:Bone and Bones / Q000033:anatomy & histology; D002910:Chronology as Topic; D005269:Femur / Q000033:anatomy & histology / Q000254:growth & development; D005456:Fluorescent Dyes; D010507:Periodicity; D010521:Periosteum / Q000033:anatomy & histology; D011893:Rana esculenta / Q000254:growth & development*; D012621:Seasons; D013194:Staining and Labeling / Q000379:methods
chemical_list: D005456:Fluorescent Dyes
vernacular_title: Mise en évidence expérimentale du caractère annuel des lignes d'arrêt de croissance squelettique chez Rana esculenta (Amphibia, anura).