In vitro proliferation of murine spleen cells. Strain variation of proliferative responses induced by recombinant IL-2.
Proliferative responses of normal (not immunized intentionally) spleen cells from inbred mouse strains to human recombinant interleukin 2 (L-2) were studied. It was shown that inbred strains vary in their responses to IL-2. The differences were observed for various doses of IL-2 and at various times after stimulation. Experimental data suggested that the strain differences in the magnitude of proliferative responses may result from an interplay of several interrelated factors such as differences in the frequency of the IL-2-responsive cells, differences in inducibility of the expression of the gene for the alpha-chain of the IL-2R, and genetic control of the responses. The latter was found to be exerted by two independent genes with the dominant alleles determining good responses to IL-2. The magnitude of the responses to IL-2 correlated with the magnitude of responses to concanavalin A, but seemed to have no effect on the interferon production or on the primary response to sheep red blood cells. The described phenomenon may have a practical significance in studies aimed at improving tumor immunotherapy with lymphokine-activated killer cells.
Zhou
P
P
Department of Microbiology, State University of New York, Buffalo.
Quackenbush
L J
LJ
Zaleski
M B
MB
D000818:Animals; D005823:Genetics; D007376:Interleukin-2 / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008815:Mice, Inbred Strains; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000494:pharmacology; D013045:Species Specificity; D013154:Spleen / Q000276:immunology*
D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D011994:Recombinant Proteins