title: Human laminin A chain (LAMA) gene: chromosomal mapping to locus 18p11.3.
abstract: Laminin, an integral component of basement membranes, consists of three subunit polypeptides, A, B1, and B2 chains. We have recently isolated cDNAs corresponding to human laminin A chain. These cDNAs were utilized for chromosomal in situ hybridizations to establish the genomic location of the laminin A chain gene. Metaphase chromosomes of PHA-stimulated human peripheral blood leukocytes were examined by in situ hybridization with 3H-labeled cDNAs, and the chromosomes were identified by R-banding (fluorochrome-photolysis-Giemsa method). The results indicated that the human laminin A chain is at locus 18p11.3. Since human laminin B1 and B2 chain genes have been previously mapped to chromosomes 7 and 1, respectively, the results indicate that genes encoding human laminin chains reside in separate chromosomes.
authors.affiliation: Department of Dermatology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania.
authors.forename: T
authors.initials: T
authors.lastname: Nagayoshi
authors.forename: M G
authors.initials: MG
authors.lastname: Mattei
authors.forename: E
authors.initials: E
authors.lastname: Passage
authors.forename: R
authors.initials: R
authors.lastname: Knowlton
authors.forename: M L
authors.initials: ML
authors.lastname: Chu
authors.forename: J
authors.initials: J
authors.lastname: Uitto
mesh_terms: D015139:Blotting, Southern; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002887:Chromosomes, Human, Pair 18; D006801:Humans; D007621:Karyotyping; D007797:Laminin / Q000235:genetics*; D015183:Restriction Mapping
chemical_list: D007797:Laminin