Demonstration of a new antinuclear antibody (anti-RA33) that is highly specific for rheumatoid arthritis.
Using immunoblot analysis with soluble nuclear extracts from HeLa cells, we identified autoantibodies to an antigen with a molecular weight of approximately 33,000 in 36% of 95 sera from rheumatoid arthritis patients, but in only 1 of 170 controls. The antigen, termed RA33, was resistant to DNase and RNase digestion but sensitive to proteinase K treatment. There was no discernible relation to other autoantibodies. Thus, this newly described autoantibody appears to be highly specific for rheumatoid arthritis.
Hassfeld
W
W
Second Department of Medicine, University of Vienna, Austria.
Steiner
G
G
Hartmuth
K
K
Kolarz
G
G
Scherak
O
O
Graninger
W
W
Thumb
N
N
Smolen
J S
JS
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000974:Antibodies, Antinuclear / Q000032:analysis / Q000276:immunology*; D000918:Antibody Specificity; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000276:immunology*; D001323:Autoantibodies / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D013582:Synovial Fluid / Q000276:immunology
D000974:Antibodies, Antinuclear; D001323:Autoantibodies