Preparation of model membranes for skin permeability studies using stratum corneum lipids.
Liposomes were prepared from stratum corneum lipids consisting of epidermal ceramides (55% by weight), cholesterol (25%), free fatty acids (15%), and cholesteryl sulfate (5%). Multiple lamellae were formed by air-drying the liposomal suspensions on hydrophilic filter disks, and water permeabilities through these filter-supported lamellae were measured using a diffusion cell. Ultrastructure of the lipid lamellae was characterized by scanning and thin-section electron microscopy. Water flux data and ultrastructure of the model membranes are discussed in relation to stratum corneum, the horny layer of the epidermis that constitutes the epidermal barrier.
Abraham
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Marshall Dermatology Research Laboratories, Department of Dermatology, University of Iowa, Iowa City.
Downing
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DT
D000818:Animals; D002463:Cell Membrane Permeability; D008051:Lipid Bilayers; D008563:Membrane Lipids / Q000502:physiology*; D008855:Microscopy, Electron, Scanning; D008954:Models, Biological; D012867:Skin / Q000166:cytology*; D013552:Swine
D008051:Lipid Bilayers; D008563:Membrane Lipids