Inhaled cigarette smoke induces preneoplastic changes in rat tracheal epithelial cells.
Rats were exposed 6 h/day, 5 days/week for 5 weeks to levels of cigarette smoke designed to deposit amounts of particulate per gram of lung similar to those experienced by a heavy smoker. Tracheal epithelial cells were isolated and examined in culture for the presence of preneoplastic variants. Two- to three-fold increases in the frequency of variants were observed in cells isolated from smoke-exposed animals compared to those from air-exposed controls. These results provide an estimate of the frequency of cigarette smoke-induced initiation events in respiratory epithelium that will be useful in further defining the role of cigarette smoke in the development of lung cancer.
Thomassen
D G
DG
Inhalation Toxicology Research Institute, Lovelace Biomedical and Environmental Research Institute, Albuquerque, NM 87185.
Chen
B T
BT
Mauderly
J L
JL
Johnson
N F
NF
Griffith
W C
WC
D000818:Animals; D004848:Epithelium / Q000187:drug effects / Q000473:pathology; D005260:Female; D008297:Male; D011230:Precancerous Conditions / Q000209:etiology* / Q000473:pathology; D051381:Rats; D011916:Rats, Inbred F344; D012016:Reference Values; D012906:Smoke / Q000009:adverse effects; D012907:Smoking / Q000009:adverse effects*; D014132:Trachea / Q000187:drug effects / Q000473:pathology*; D014134:Tracheal Neoplasms / Q000209:etiology* / Q000473:pathology
D012906:Smoke