Synergism between lysophosphatidylserine and the phorbol ester tetradecanoylphorbolacetate in rat mast cells.
In rat peritoneal mast cells tetradecanoylphorbolacetate (TPA) induced a non cytotoxic histamine release in the absence of extracellular calcium. The addition of calcium prevented the TPA effect but micromolar concentrations of lysophosphatidylserine (lysoPS) converted the calcium-induced inhibition into a stimulation. Other lysophospholipids were inactive. In agreement with a mutual influence between lysoPS and TPA, minimal TPA concentrations enhanced the calcium-dependent histamine release induced by lysoPS in the presence of nerve-growth factor. It is proposed that the calcium-dependent pathway promoted by lysoPS and the activation of protein kinase C by TPA act synergically to induce histamine release from mast cells.
Battistella
A
A
Mietto
L
L
Toffano
G
G
Palatini
P
P
Bigon
E
E
Bruni
A
A
D000818:Animals; D002118:Calcium / Q000378:metabolism; D004357:Drug Synergism; D004492:Edetic Acid / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects; D008246:Lysophospholipids; D008297:Male; D008407:Mast Cells / Q000187:drug effects*; D010704:Phorbols / Q000494:pharmacology*; D010718:Phosphatidylserines / Q000494:pharmacology*; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D008246:Lysophospholipids; D010704:Phorbols; D010718:Phosphatidylserines; C025059:lysophosphatidylserine; D004492:Edetic Acid; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium