Reactions of isolated rat aorta to X-irradiation.
The reaction of isolated helical strips of rat aorta to X-irradiation was studied: X-rays (50 kV) induced a reproducible, reversible contractile response at threshold doses of 2.5 Gy (60 Gy/min) and 10 Gy (30 Gy/min). After repeated irradiation with the same doses at equal time intervals and constant dose-rate (for example 50 Gy every 15 min, dose-rate 60 Gy/min) the contractile response was progressively diminished, i.e. a tachyphylaxis appeared. The preparations still reacted even after total doses over 1000 Gy. The X-ray induced contractile responses were dose- and dose-rate dependent. Quantitative analysis indicated no essential differences in the radiation-induced contractile response when recorded under isometric or isotonic conditions. After hypothermia (20 degrees C) or hyperthermia (42 degrees C) no visible radiation response could be induced. Part of the aortic strips were spontaneously active: X-ray doses of 5 to 10 Gy stimulated, while doses of 100 to 200 Gy inhibited or blocked the spontaneous phasic activity. A comparison between the immediate X-ray reaction of vascular and non-vascular smooth muscle preparations is given. Participation of cholinergic and adrenergic mechanisms in the X-ray induced contractions of rat aorta seems to be ruled out, because the blocking agents atropine, phentolamine, and bretylium had no effect.
Michailov
M C
MC
Gesellschaft für Strahlen- und Umweltforschung, Neuherberg bei München.
Amelung
H
H
Welscher
U
U
Neu
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E
D000818:Animals; D001011:Aorta / Q000528:radiation effects; D001285:Atropine / Q000494:pharmacology; D066298:In Vitro Techniques; D007537:Isometric Contraction / Q000528:radiation effects; D009119:Muscle Contraction / Q000187:drug effects / Q000528:radiation effects*; D009131:Muscle, Smooth, Vascular / Q000528:radiation effects*; D051381:Rats; D011919:Rats, Inbred Strains; D013618:Tachyphylaxis / Q000528:radiation effects; D013696:Temperature
D001285:Atropine