Differential gene activity visualized on sister chromatids after replication in the presence of 5-azacytidine.
Mitotic chromosomes with sister chromatids bearing differentially active ribosomal gene clusters were recovered from human lymphocytes exposed to 5-azacytidine. The hypothesis was that the differential activity was determined by the hypomethylation of one of the two sister chromatids. The verification was carried out by "labeling" the 5-azacytidine-substituted chromatid with BUdR, and then checking the location of active clusters by specific staining techniques. Data obtained confirmed that the chromatid bearing the active cluster was indeed the 5-azacytidine-substituted one.
Ferraro
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M
Lavia
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P
D001374:Azacitidine / Q000494:pharmacology*; D004261:DNA Replication; D004275:DNA, Ribosomal / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D008214:Lymphocytes; D008745:Methylation; D009697:Nucleolus Organizer Region / Q000187:drug effects / Q000648:ultrastructure
D004275:DNA, Ribosomal; D001374:Azacitidine