Identification of a voltage-dependent anion channel in the apical membrane of a Cl(-)-secretory epithelium (MDCK).
An anion-selective channel of large unit conductance is present in apical membranes of a secretory epithelial cell. For cell-attached patch-clamp configuration on cultured MDCK cells channels with a conductance of about 460 pS are observed at 37 degrees C. The channel becomes spontaneously activated only in rare cases. In inside-out membrane patches the probability of observing this type of channel increased significantly suggesting that the channel is controlled by an up to now unknown mechanism. The channel shows a voltage-dependent burst kinetic.
Kolb
H A
HA
Brown
C D
CD
Murer
H
H
D000838:Anions / Q000378:metabolism* / Q000502:physiology; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D002712:Chlorides / Q000378:metabolism*; D004594:Electrophysiology; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D007473:Ion Channels / Q000502:physiology*; D007668:Kidney / Q000166:cytology / Q000378:metabolism*
D000838:Anions; D002712:Chlorides; D007473:Ion Channels