Chronic progressive myelopathy associated with HTLV-I: oligoclonal IgG and anti-HTLV-I IgG antibodies in cerebrospinal fluid and serum.
Among 22 patients with human T-lymphotropic virus type I (HTLV-I)-associated chronic progressive myelopathy, agarose isoelectric focusing (AIF) revealed oligoclonal IgG bands in 21: in 3 in CSF only; in 11 in CSF and to some extent in serum; and in 7, identical patterns in CSF and serum. By immunoblot after AIF of CSF and serum, we observed bands of anti-HTLV-I IgG antibodies in 19 patients: in 5 in CSF only; in 9 in CSF and partly in serum; and in 5, identical in CSF and serum. Oligoclonal anti-HTLV-I IgG antibody bands could only partly be traced to oligoclonal IgG bands. If, prior to AIF, serum and CSF were absorbed with HTLV-I antigen, practically all oligoclonal HTLV-I-specific IgG antibody activity was abolished, while the oligoclonal pattern of total IgG was affected only to a minor extent. Alongside with HTLV-I-specific oligoclonal B cell response, HTLV-I myelopathy is regularly accompanied by production of oligoclonal IgG of unknown antibody specificities.
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Department of Neurology, Karolinska Institutet, Huddinge University Hospital, Stockholm, Sweden.
Cruz
M
M
Gessain
A
A
Gout
O
O
de Thé
G
G
Kam-Hansen
S
S
D000328:Adult; D000368:Aged; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis / Q000134:cerebrospinal fluid*; D015368:Human T-lymphotropic virus 1 / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000032:analysis / Q000134:cerebrospinal fluid; D007136:Immunoglobulins / Q000032:analysis; D008875:Middle Aged; D043025:Oligoclonal Bands; D015493:Paraparesis, Tropical Spastic / Q000097:blood / Q000134:cerebrospinal fluid* / Q000175:diagnosis; D012698:Serologic Tests
D000888:Antibodies, Anti-Idiotypic; D007074:Immunoglobulin G; D007136:Immunoglobulins; D043025:Oligoclonal Bands