Interstrain restriction fragment length polymorphism in the c-src correlates with Na,K cotransport and calcium content in hybrid rat erythrocytes.
Twenty-six F2 hybrids between spontaneously hypertensive (SHR) and normotensive control (WKY) rats [(SHR x WKY)F2] were segregated according to their c-src genotype into SS and WW homozygous groups, corresponding to SHR or WKY and a WS heterozygous group. The Na,K cotransport in erythrocytes in the WW group was equal to that of WKY and differed significantly from that of the WS and SS groups (the rate of Na,K cotransport in the latter groups was close to that of the SHR). The calcium content of the erythrocytes in the WW group was equal to that of the WKY, but lower than that of the WS and SS groups which, in turn, was significantly lower than that in the SHR, indicating polygenic control of the trait. We concluded that the c-src locus itself or some other loci inherited in conjunction with the c-src determines the increase in Na,K cotransport and in calcium content in erythrocytes of SHR.
Kotelevtsev
Y V
YV
Central Research Laboratory, Ministry of Public Health, Moscow, USSR.
Spitkovski
D D
DD
Orlov
S N
SN
Postnov
Y V
YV
D000818:Animals; D001692:Biological Transport / Q000502:physiology; D002118:Calcium / Q000097:blood*; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D005838:Genotype; D006823:Hybrid Vigor / Q000502:physiology*; D006824:Hybridization, Genetic / Q000502:physiology*; D008297:Male; D009857:Oncogenes / Q000502:physiology*; D012150:Polymorphism, Restriction Fragment Length; D011188:Potassium / Q000097:blood*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D012964:Sodium / Q000097:blood*
D012964:Sodium; D011188:Potassium; D002118:Calcium