Glutamine assimilation pathways in Neurospora crassa growing on glutamine as sole nitrogen and carbon source.
Neurospora crassa wild-type is almost unable to grow on glutamine as sole nitrogen and carbon source but a GDH-; GS +/- double mutant strain, lacking NADP-dependent glutamate dehydrogenase and partially lacking glutamine synthetase did grow. Under these conditions, the double mutant had a higher chemical energy content than the wild-type. Enzyme assays and labelling experiments with glutamine indicated that in the double mutant glutamine was degraded to ammonium and to carbon skeletons by glutamate synthase, the catabolic (NADH-dependent) glutamate dehydrogenase and the glutamine transaminase-omega-amidase pathway.
Calderón
J
J
Departamento de Ecología Molecular, Centro de Investigación sobre Fijación de Nitrógeno, Universidad Nacional Autónoma de México, Cuernavaca, Morelos.
Mora
J
J
D000596:Amino Acids / Q000378:metabolism; D000641:Ammonia / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D004734:Energy Metabolism; D005969:Glutamate Dehydrogenase / Q000172:deficiency; D005970:Glutamate Synthase / Q000378:metabolism; D005974:Glutamate-Ammonia Ligase / Q000172:deficiency; D005973:Glutamine / Q000378:metabolism*; D009154:Mutation; D009491:Neurospora / Q000378:metabolism*; D009492:Neurospora crassa / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D000637:Transaminases / Q000378:metabolism
D000596:Amino Acids; D005973:Glutamine; D002244:Carbon; D000641:Ammonia; D005970:Glutamate Synthase; D005969:Glutamate Dehydrogenase; D000637:Transaminases; C020656:glutamine-pyruvate aminotransferase; D005974:Glutamate-Ammonia Ligase; D009584:Nitrogen