Synthesis of 9 alpha-hydroxysteroids by a Rhodococcus sp.
9 alpha-Hydroxylation of delta 5-3 beta-hydroxysteroids (of androstane, pregnane, 24-nor- and 21,24-bisnorcholane groups) was carried out by a Rhodococcus sp., isolated from a petroleum-containing soil sample. A large number of the investigated steroids was transformed into 9 alpha-hydroxy-delta 4-3-ketones in satisfactory yields (50-90%) at high initial concentrations of the substrates (0.5-5.0 g/L). The influence of some structural features of the steroid molecule on the progress and effectiveness of the microbial transformation was also shown.
Datcheva
V K
VK
Institute of Organic Chemistry with Centre of Phyto-Chemistry, Bulgarian Academy of Sciences, Sofia.
Voishvillo
N E
NE
Kamernitskii
A V
AV
Vlahov
R J
RJ
Reshetova
I G
IG
D000731:Androstanes / Q000378:metabolism; D001711:Biotransformation; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D006900:Hydroxylation; D006914:Hydroxysteroids / Q000378:metabolism*; D011278:Pregnanes / Q000378:metabolism; D012240:Rhodococcus / Q000378:metabolism*
D000731:Androstanes; D006914:Hydroxysteroids; D011278:Pregnanes