Chromosome localization and cDNA sequence of murine and human genes for ras p21 GTPase activating protein (GAP).
The cDNA coding for mouse and human ras p21 GTPase-activating protein (GAP) was isolated; the deduced amino acid sequences share over 96% homology with that previously determined for bovine brain GAP. Both the mouse and human GAP cDNAs were used as probes for the chromosomal localization of this gene. The locus designations for the gene encoding GAP in human and mouse are RASA and Rasa (for ras-activating protein), respectively. By somatic cell hybrid analysis and in situ chromosomal hybridization, we have assigned the RASA gene to human chromosome band 5q13.3. In addition, with somatic cell genetics and linkage analysis in recombinant inbred mouse strains, the murine Rasa gene was localized to the distal end of mouse chromosome 13. These assignments place the gene encoding GAP in a known conserved syntenic region.
Hsieh
C L
CL
Howard Hughes Medical Institute, Stanford University Medical Center, California 94305-5428.
Vogel
U S
US
Dixon
R A
RA
Francke
U
U
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002895:Chromosomes, Human, Pair 5; D003001:Cloning, Molecular; D004247:DNA; D015342:DNA Probes; D020690:GTPase-Activating Proteins; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length; D011506:Proteins / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D020703:ras GTPase-Activating Proteins
D015342:DNA Probes; D020690:GTPase-Activating Proteins; D011506:Proteins; D020703:ras GTPase-Activating Proteins; D004247:DNA