Cell density and cell shape-related regulation of vimentin and cytokeratin synthesis. Inhibition of vimentin synthesis and appearance of a new 45 kD cytokeratin in dense epithelial cell cultures.
The pattern of the intermediate type filament protein synthesis was examined in cultured bovine mammary gland epithelial (BMGE) cells under conditions of varied cell shape and cell-cell contact. In dense monolayer and suspension cultures BMGE cells expressed a new cytokeratin of 45 kD identified as a member of the acidic subfamily of cytokeratins. This polypeptide has a phosphorylated component and is dissociated from the cytokeratins complex in the presence of 6.5 M urea. The mRNA of the new cytokeratin accumulated in dense cell cultures, as revealed by in vitro translation in a cell-free system. In BMGE-H cells that express also vimentin, the synthesis of vimentin decreased dramatically in dense cell cultures, while the synthesis of the 45 kD cytokeratin was maximal under these conditions. The results suggest that the expression of certain cytokeratins and that of vimentin can be coordinately regulated by factors in the cellular environment that effect cell shape and cell surface contacts.
Ben-Ze'ev
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D000818:Animals; D002417:Cattle; D002450:Cell Communication; D002452:Cell Count; D002460:Cell Line; D003599:Cytoskeleton / Q000378:metabolism*; D004848:Epithelium; D007633:Keratins / Q000096:biosynthesis* / Q000235:genetics; D008321:Mammary Glands, Animal; D008970:Molecular Weight; D010766:Phosphorylation; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D014508:Urea / Q000494:pharmacology; D014746:Vimentin / Q000096:biosynthesis*
D012333:RNA, Messenger; D014746:Vimentin; D007633:Keratins; D014508:Urea