In-utero transplantation of fetal liver haemopoietic stem cells in monkeys.
To evaluate the potential of in-utero transplantation of fetal haemopoietic stem cells (HSCs) for permanent engraftment as a treatment of congenital haemoglobinopathies, fetal rhesus monkeys were transplanted with HSCs derived from fetal livers. Five pregnant monkeys (60-62 days' gestation) were given an in-utero intraperitoneal injection of fetal liver cells (10(8)-10(9) cells/kg estimated fetal recipient body weight) derived from opposite sex donors at 59-68 days' gestation. Engraftment was confirmed by karyotype analysis of peripheral blood leucocytes and bone marrow; cells of donor sex were found among the recipient cells. Donor cell engraftment was apparent in four of five in-utero HSC transplant recipients at birth. Engraftment involved lymphoid (2.9-8.0% donor cells), erythroid (5.3-12.5%), and myeloid (8.5-15.4%) lineages and has persisted for up to 2 years without evidence of graft-versus-host disease.
Harrison
M R
MR
Department of Surgery, University of California, San Francisco.
Slotnick
R N
RN
Crombleholme
T M
TM
Golbus
M S
MS
Tarantal
A F
AF
Zanjani
E D
ED
D000367:Age Factors; D000818:Animals; D002478:Cells, Cultured; D002678:Chimera / Q000235:genetics; D015672:Erythroid Precursor Cells / Q000032:analysis; D005069:Evaluation Studies as Topic; D005260:Female; D005333:Fetus; D005500:Follow-Up Studies; D018380:Hematopoietic Stem Cell Transplantation; D006453:Hemoglobinopathies / Q000151:congenital / Q000628:therapy; D006801:Humans; D007621:Karyotyping; D008099:Liver / Q000166:cytology*; D008253:Macaca mulatta; D008297:Male; D011247:Pregnancy; D014184:Transplantation, Homologous