title: Monoclonal antibody VC1.1 selectively stains a population of GABAergic neurons containing the calcium-binding protein parvalbumin in the rat cerebral cortex.
abstract: Monoclonal antibody VC1.1 is shown to stain selectively a subpopulation of GABAergic neurons in the rat cerebral cortex. Almost all VC1.1 immunoreactive cells were also GABA-like immunoreactive (GABA-LI) and parvalbumin (PV) immunoreactive, whereas they were about 30% and 65% of GABA-LI and PV-positive cells in the parietal cortex and about 13% and 32% in the occipital cortex, respectively. Although a few VC1.1 positive cells showed somatostatin-like and/or cholecystokinin-like immunoreactivities, they were exceptional (less than 1% of VC1.1 positive cells). Furthermore about 90% of VC1.1 positive cells were also stained with a lectin, Vicia villosa agglutinin, with a specific affinity for terminal N-acetylgalactosamine.
authors.affiliation: National Institute for Physiological Sciences, Okazaki, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kosaka
authors.forename: C W
authors.initials: CW
authors.lastname: Heizmann
authors.forename: C J
authors.initials: CJ
authors.lastname: Barnstable
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D009124:Muscle Proteins / Q000378:metabolism*; D010320:Parvalbumins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D002135:Calcium-Binding Proteins; D009124:Muscle Proteins; D010320:Parvalbumins; D005680:gamma-Aminobutyric Acid