Insoluble disulfide cross-linked polypeptides accumulate in the functionally compromised lysosomes of fibroblasts treated with the cysteine protease inhibitor E-64.
Mouse fibroblasts (3T3-L1 cells) accumulate pulse-labeled long-lived polypeptides in detergent- and salt-insoluble aggregates when chased in the presence of inhibitors of lysosomal cysteine cathepsins, including E-64. Proteins found in the detergent- and salt-insoluble fraction include polypeptides which are disulfide cross-linked. E-64-induced polypeptide aggregates cofractionate with lysosomal enzyme markers on density gradients and are found in multivesicular dense bodies which by electron microscopy appear to be engaged in microautophagy. The results are discussed in relation to the possible role of polypeptide aggregation in the sequestration or trapping of cytoplasmic proteins by the lysosomal system.
Doherty
F J
FJ
Department of Biochemistry, University of Nottingham Medical School, United Kingdom.
Osborn
N U
NU
Wassell
J A
JA
Laszlo
L
L
Mayer
R J
RJ
D000818:Animals; D002478:Cells, Cultured; D003432:Cross-Linking Reagents; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology*; D007700:Kinetics; D007930:Leucine / Q000031:analogs & derivatives* / Q000494:pharmacology; D008247:Lysosomes / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D051379:Mice; D008854:Microscopy, Electron; D008970:Molecular Weight; D010455:Peptides / Q000302:isolation & purification / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism
D003432:Cross-Linking Reagents; D015853:Cysteine Proteinase Inhibitors; D010455:Peptides; D011506:Proteins; D007930:Leucine; C024974:E 64