title: Phospholipids differently modulate the activity of cytosolic protein-tyrosine kinase from porcine spleen.
abstract: Effect of membrane phospholipids on the activity of cytosolic protein-tyrosine kinase from porcine spleen (CPTK-40) has been studied. Using poly(Glu Na, Tyr)4:1 as a substrate, phosphatidylethanolamine, phosphatidylcholine and phosphatidylserine had stimulatory effects on that phosphorylation activity, however phosphatidic acid had inhibitory and phosphatidylinositol had no effects. Similar results were obtained using[Val5]angiotensin II as a substrate. On the other hand using basic protein (H2B histone and myelin basic protein) as substrates, phosphatidic acid stimulated the activity of CPTK-40, while phosphatidylinositol inhibited the activity. Phosphatidylethanolamine, phosphatidylcholine and phosphatidylserine caused different effect on the activity of CPTK-40 depending on the substrate employed. However using acidic protein (tubulin and casein) as substrates, the activity of CPTK-40 was neither stimulated nor inhibited by any phospholipids. These results suggest that phospholipids may modulate the activity of CPTK-40.
authors.affiliation: Department of Biochemistry, Fukui Medical School, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Sakai
authors.forename: T
authors.initials: T
authors.lastname: Taniguchi
authors.forename: R
authors.initials: R
authors.lastname: Shimomura
authors.forename: M
authors.initials: M
authors.lastname: Asahi
authors.forename: T
authors.initials: T
authors.lastname: Kobayashi
authors.forename: T
authors.initials: T
authors.lastname: Inazu
authors.forename: S
authors.initials: S
authors.lastname: Nakamura
authors.forename: H
authors.initials: H
authors.lastname: Yamamura
mesh_terms: D000818:Animals; D003600:Cytosol / Q000201:enzymology; D007700:Kinetics; D008051:Lipid Bilayers; D010743:Phospholipids / Q000494:pharmacology*; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D013154:Spleen / Q000201:enzymology*; D013552:Swine
chemical_list: D008051:Lipid Bilayers; D010743:Phospholipids; D011505:Protein-Tyrosine Kinases