[Preparation of catalytic subunits of cAMP-dependent protein kinase from cattle myocardium].
A method is suggested for obtaining a catalytic subunit of cAMP-dependent protein kinase from the cattle myocardium. The specific activity of protein kinase is 0.5 mumol 32P per 1 mg of the enzyme. The method is based on the difference of protein kinase in the subunit and choloenzyme charges, it embraces the stages of homogenization, ultracentrifugation and biospecific elution on anion exchanger of DEAE-Sepharose-CL-6B using 10(-4) M cAMP in the stationary variant.
Babich
L G
LG
Bychenok
S F
SF
Kondratiuk
T P
TP
D000818:Animals; D002384:Catalysis; D002417:Cattle; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D009132:Muscles / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology*; D011494:Protein Kinases / Q000302:isolation & purification / Q000378:metabolism*; D011817:Rabbits
D011494:Protein Kinases
Poluchenie kataliticheskoĭ sub''edinitsy cAMP-zavisimoĭ proteinkinazyiz miokarda krupnogo rogatogo skota.