Immunogenetic aspects of a canine breeding colony.
A colony of dogs was expanded by selective breeding to study the immunogenetic determinants coded for by the major histocompatibility complex (DLA). Polymorphic determinants were identified by alloantisera specific for DLA-A and B loci antigens and by the mixed lymphocyte culture (MLC) which defined alleles at the D locus. Thirteen families totaling 58 offspring were produced and typed for allelic determinants coded for by each of the three gene loci. Allelic segregation in a codominant manner occurred as expected and a recombinant between the A and B loci was detected. A number of animals were homozygous at one or more loci, thus providing genetically standardized animals as a source of typing cells, antigens, and sera to further study the immunogenetic details of DLA and for in vivo studies in transplantation biology.
Ladiges
W C
WC
Deeg
H J
HJ
Raff
R F
RF
Storb
R
R
D000483:Alleles; D000818:Animals; D001947:Breeding; D004285:Dogs / Q000235:genetics / Q000276:immunology*; D000939:Epitopes / Q000235:genetics; D005260:Female; D006579:Heterozygote; D006649:Histocompatibility Antigens / Q000235:genetics; D006720:Homozygote; D007959:Lymphocyte Culture Test, Mixed; D008285:Major Histocompatibility Complex; D008297:Male; D012641:Selection, Genetic
D000939:Epitopes; D006649:Histocompatibility Antigens