title: Quantitative determination of itazigrel in rodent diet by reversed-phase HPLC and evaluation of its stability at 20 degrees C.
abstract: A simple, accurate and precise procedure for the quantitation of itazigrel (a potent lipophilic inhibitor of collagen and arachidonic acid-induced aggregation being studied for its effects on peripheral vascular disease) from granulated rodent diet is presented. The drug was extracted from rodent diet using methanol + water (80:20) following dissolution of the diet in water. Samples of the supernatant were injected into the HPLC and the eluent was monitored with a fluorescent detector (lambda ex = 320 and lambda em = 430) to achieve analytical specificity. Interday coefficients of variation of the calibration curve slope were +/- 6% on standards between 0 and 1000 micrograms/g. Potency and homogeneity of the drug spiked diet prepared over a 1 year interval at 70, 200 and 600 micrograms/g was 99.3 +/- 2.5%, 100 +/- 1.8%, and 101 +/- 1.9% of label, respectively. Samples prepared for chromatography were stable for 24 h at 20 degrees C, and drug in diet was stable for 102 days when protected from light and stored at 20 degrees C.
authors.affiliation: Biofluids Analytical Laboratories, Upjohn Company, Kalamazoo, MI 49001.
authors.forename: P A
authors.initials: PA
authors.lastname: Bombardt
authors.forename: B E
authors.initials: BE
authors.lastname: Bothwell
authors.forename: S K
authors.initials: SK
authors.lastname: Closson
authors.forename: W J
authors.initials: WJ
authors.lastname: Adams
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D012377:Rodentia; D013050:Spectrometry, Fluorescence; D013696:Temperature; D013844:Thiazoles / Q000032:analysis* / Q000633:toxicity
chemical_list: D013844:Thiazoles; C035025:itazigrel