An alternative approach for induction of chromosomal banding and preservation of chromosomal morphology for virus DNA mapping by in situ hybridization.
I report on the use of acetic/saline/Giemsa technique for the induction of chromosome banding and an additional fixation (prior hybridization) to preserve the chromosome morphology as regards in situ hybridization under stringent conditions. This approach results in high quality banding resolution for grain localization of integrated sites of virus DNA sequences on both metaphase and prometaphase chromosomes.
Mincheva
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A
Research Institute of Infectious and Parasitic Diseases, Department of Virology, Medical Academy, Sofia, Bulgaria.
D002871:Chromosome Banding; D002877:Chromosomes, Human / Q000648:ultrastructure*; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis*; D006801:Humans; D008677:Metaphase; D027383:Papillomaviridae / Q000235:genetics*; D011309:Preservation, Biological; D014407:Tumor Cells, Cultured
D015344:DNA Probes, HPV; D004279:DNA, Viral