Establishment of mouse and rat hepatoma cell clones showing stable expression of rabbit cytochrome P450 IA2.
Cytochrome P450 IA2, a liver-specific member of the 3-methylcholanthrene-inducible family, is never detected in established cell lines. With the aim of isolating cells stably producing this protein, we have used rat and mouse hepatoma cells as recipients in transfection experiments involving rabbit cytochrome P450 IA2 cDNA. We report here the isolation of five hepatoma cell clones expressing functional P450 IA2. The level of expression is comparable to that found in COS cells transiently transformed by other P450 cDNAs. It ranges between 0.4 and 1.6 pmol P450 IA2/mg total cell protein.
Corcos
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Unité de Génétique de la Différenciation, URA 152 du CNRS, Département de Biologie Moléculaire, Paris, France.
Rechenmann
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Weiss
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Pompon
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D000818:Animals; D015139:Blotting, Southern; D002999:Clone Cells; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D008099:Liver / Q000201:enzymology; D008114:Liver Neoplasms, Experimental; D051379:Mice; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D051381:Rats; D013045:Species Specificity; D014162:Transfection; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D003577:Cytochrome P-450 Enzyme System