Effect of actinomycin D on the expression of nuclear antigen SS-B/La.
The nuclear autoantigen SS-B/La, which is recognized by antibodies in certain autoimmune diseases, is complexed with distinct cellular RNAs and the complex of antigen-RNA profile remains constant throughout the cell cycle. This antigen is prominent in the nucleolus during G1/S phase of the cell cycle. Actinomycin D at a concentration of 0.0005 microgram/ml is capable of abolishing the appearance of SS-B/La in the nucleolus. The amount of immune-precipitable SS-B/La-associated RNAs is reduced by 0.005 microgram/ml actinomycin D treatment. The mechanism for these findings is suggested to be related to inhibition of the synthesis of messenger RNA coding for SS-B/La.
Deng
J S
JS
Tan
E M
EM
D000941:Antigens / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology*; D001402:B-Lymphocytes; D002453:Cell Cycle; D002460:Cell Line; D002467:Cell Nucleus / Q000276:immunology*; D011232:Chemical Precipitation; D003609:Dactinomycin / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007120:Immunochemistry; D008856:Microscopy, Fluorescence; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis / Q000378:metabolism; D012342:RNA, Small Nuclear; D012261:Ribonucleoproteins; D000097585:SS-B Antigen
D000941:Antigens; D001324:Autoantigens; D003609:Dactinomycin; D012313:RNA; D012342:RNA, Small Nuclear; D012261:Ribonucleoproteins; D000097585:SS-B Antigen