Hydrostatic pressure studies of native and synthetic thick filaments: in vitro myosin aggregates at pH 7.0 with and without C-protein.
Column-purified myosin at pH 7.0 will reproducibly aggregate into filaments of known average length and structure when dialyzed against a low ionic strength medium under controlled conditions. When exposed to increased hydrostatic pressure, followed by quick return to atmospheric pressure, the original filaments shorten linearly with increasing pressure; in addition, a second population of filaments is seen, presumably the result of reaggregation of myosin after release of pressure. This second population is about 0.5 microns long, bipolar, and about half the diameter of the original filaments. The number of these filaments, but not their physical characteristics, is a function of the shortening of the original filament population. Both the remnants of the original population and the new aggregates, once formed, are stable over time and at room temperature. The addition of C-protein to myosin solutions before filament preparation results in a filament population of slightly shorter length. When these filaments are exposed to increased hydrostatic pressure, they are more resistant to disaggregation than myosin filaments without C-protein. However, like the filaments prepared in the absence of C-protein, a second population of shorter, thinner filaments is visible after exposure to pressure.
Tumminia
S J
SJ
Center for Biophysics, Rensselaer Polytechnic Institute, Troy, NY 12180-3590.
Koretz
J F
JF
Landau
J V
JV
D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000032:analysis; D002352:Carrier Proteins; D003956:Dialysis; D006863:Hydrogen-Ion Concentration; D006874:Hydrostatic Pressure; D008854:Microscopy, Electron; D009132:Muscles / Q000032:analysis* / Q000648:ultrastructure; D009218:Myosins / Q000032:analysis* / Q000648:ultrastructure; D009391:Nephelometry and Turbidimetry; D011312:Pressure; D011817:Rabbits
D002352:Carrier Proteins; C020723:citrate-binding transport protein; D017301:Ca(2+) Mg(2+)-ATPase; D009218:Myosins