Head activator acts as an autocrine growth factor for NH15-CA2 cells in the G2/mitosis transition.
The neuropeptide head activator (HA) acts as an autocrine growth factor for the neural cell line NH15-CA2. Cell proliferation is increased in the presence of HA and inhibited by HA peptide-specific antisera. Stimulation of cellular proliferation is visible 2 h after HA application as an increase in cells in mitosis. HA has no direct effect on stimulating DNA synthesis. HA thus functions as a control signal in the G2/mitosis transition and not in the G1/S transition. Receptors for HA are present on small round cells in clusters of foci and not on cells with differentiated morphology, suggesting cell-cycle-dependent HA receptor expression.
Schaller
H C
HC
Zentrum für Molekulare Biologie, Heidelberg, FRG.
Druffel-Augustin
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S
Dübel
S
S
D000818:Animals; D001665:Binding Sites; D002455:Cell Division; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000096:biosynthesis; D005910:Glioma; D006133:Growth Substances / Q000494:pharmacology*; D006822:Hybrid Cells; D007150:Immunohistochemistry; D007399:Interphase; D051379:Mice; D008938:Mitosis; D009447:Neuroblastoma; D009474:Neurons / Q000166:cytology*; D009479:Neuropeptides / Q000494:pharmacology*; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D051381:Rats; D014407:Tumor Cells, Cultured
D006133:Growth Substances; D009479:Neuropeptides; C032522:head activator peptide; D004247:DNA; D011761:Pyrrolidonecarboxylic Acid