Somatostatinergic neurons in the insular cortex project to the spinal cord: combined retrograde axonal transport and immunohistochemical study.
A double-labeling method combining immunohistochemistry and a retrograde tracer technique using biotin-horseradish peroxidase (B-HRP) was employed to identify a descending somatostatinergic fiber system from the insular cortex to the spinal cord. Injection of B-HRP into the spinal cord at cervical or lumbar levels resulted in the labeling of a number of neurons in the insular cortex. Simultaneous immunostaining revealed the existence of double-labeled neurons in the insular cortex. The result provides direct evidence for the presence of a descending somatostatinergic pathway from the insular cortex to lumbar levels of the spinal cord.
Shimada
S
S
Shiosaka
S
S
Takami
K
K
Yamano
M
M
Tohyama
M
M
D000818:Animals; D001370:Axonal Transport; D002540:Cerebral Cortex / Q000378:metabolism* / Q000502:physiology; D004525:Efferent Pathways / Q000502:physiology; D005455:Fluorescent Antibody Technique; D051381:Rats; D011919:Rats, Inbred Strains; D013004:Somatostatin / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism* / Q000502:physiology; D009435:Synaptic Transmission
D013004:Somatostatin