Induction of antitumor immunity in mice by allo-major histocompatibility complex class I gene transfectant with strong antigen expression.
An allo-major histocompatibility complex class I gene (H-2Kb) was transfected to murine mastocytoma P1.HTR (P815 subline) cells, after which several transfectant clones were obtained. Two clones, which expressed a low level of H-2Kb antigen, grew well and killed the syngeneic DBA/2 mice when they were inoculated ip. These mice lived longer than the mice given injections of the parental P1.HTR tumor. However, one clone, which expressed a high level of H-2Kb antigen, was rejected completely by the syngeneic DBA/2 mice and induced a generation of H-2Kb-specific cytotoxic T cells. Interestingly, the mice that had rejected the clone with high H-2Kb expression received strong anti-tumor immunity for rejection of the parental P1.HTR tumor challenged at the high dose.
Isobe
K
K
Department of Immunology, Nagoya University School of Medicine, Japan.
Hasegawa
Y
Y
Iwamoto
T
T
Hasegawa
T
T
Kawashima
K
K
Ding
L N
LN
Nakashima
I
I
D000818:Animals; D000941:Antigens / Q000032:analysis*; D015197:Carcinogenicity Tests; D002999:Clone Cells; D006084:Graft Rejection; D007942:Leukemia, Experimental / Q000235:genetics / Q000276:immunology*; D008285:Major Histocompatibility Complex / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009368:Neoplasm Transplantation; D010957:Plasmids / Q000235:genetics; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014162:Transfection / Q000276:immunology*; D014181:Transplantation Immunology; D014407:Tumor Cells, Cultured
D000941:Antigens