Surfactant enhancement of polyethyleneglycol-induced cell fusion.
B16 mouse melanoma cells in monolayers may be satisfactorily fused with 50% PEG 1500. However, pre-treatment with detergents in solution at low concentrations significantly increases PEG fusion, up to 8-fold in some instances, without impairing cell viability. The practical and mechanistical implications of this finding are discussed.
Prado
A
A
Department of Cell Biology, University of Basque Country, Bilbao, Spain.
Partearroyo
M A
MA
Mencía
M
M
Goñi
F M
FM
Barberá-Guillem
E
E
D000818:Animals; D002459:Cell Fusion / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D003902:Detergents / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D051379:Mice; D011092:Polyethylene Glycols / Q000494:pharmacology*; D013501:Surface-Active Agents / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D003902:Detergents; D013501:Surface-Active Agents; D011092:Polyethylene Glycols