title: Cloned mouse ribonucleotide reductase subunit M1 cDNA reveals amino acid sequence homology with Escherichia coli and herpesvirus ribonucleotide reductases.
abstract: We have isolated and sequenced overlapping cDNA clones containing the entire coding region of mouse ribonucleotide reductase subunit M1. The coding region comprises 2.4 kilobases and predicts a polypeptide of 792 amino acids (Mr 90,234) which shows striking homology with ribonucleotide reductases from Escherichia coli and the herpesviruses, Epstein-Barr virus and herpes simplex virus. The homologies reveal three domains: an N-terminal domain common to the mammalian and bacterial enzymes, a C-terminal domain common to the mammalian and viral ribonucleotide reductases, and a central domain common to all three. We speculate on the functional basis of this conservation.
authors.forename: I W
authors.initials: IW
authors.lastname: Caras
authors.forename: B B
authors.initials: BB
authors.lastname: Levinson
authors.forename: M
authors.initials: M
authors.lastname: Fabry
authors.forename: S R
authors.initials: SR
authors.lastname: Williams
authors.forename: D W
authors.initials: DW
authors.lastname: Martin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D004926:Escherichia coli / Q000201:enzymology*; D004854:Herpesvirus 4, Human / Q000201:enzymology; D046911:Macromolecular Substances; D051379:Mice; D011061:Poly A / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger; D012264:Ribonucleotide Reductases / Q000235:genetics*; D018139:Simplexvirus / Q000201:enzymology*
chemical_list: D046911:Macromolecular Substances; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004247:DNA; D012264:Ribonucleotide Reductases