title: Human vascular permeability factor. Isolation from U937 cells.
abstract: Human vascular permeability factor (hVPF) is a glycoprotein that promotes fluid and protein leakage from blood vessels. The function of hVPF is at present unknown, but the potent bioactivities of this protein suggest that it could act during inflammation, wound healing, and tumor angiogenesis. hVPF was purified from serum-free conditioned medium of the human histiocytic lymphoma cell line U937 as a disulfide-linked dimeric 40-kDa protein that promoted dermal blood vessel leakage in guinea pigs at a dose of 20 ng (3 x 10(-9) M) and promoted in vitro endothelial cell growth at concentrations as low as 50 PM. Multiple forms of hVPF with apparent pI values greater than 7.5 were resolved using pH gradient electrophoresis. Antibodies against guinea pig vascular permeability factor were found to cross-react with hVPF. The N-terminal amino acid sequence of hVPF was similar to, but not identical with, the N-terminal sequence of guinea pig vascular permeability factor.
authors.affiliation: Department of Cell Culture, Monsanto Company, St. Louis, Missouri 63167.
authors.forename: D T
authors.initials: DT
authors.lastname: Connolly
authors.forename: J V
authors.initials: JV
authors.lastname: Olander
authors.forename: D
authors.initials: D
authors.lastname: Heuvelman
authors.forename: R
authors.initials: R
authors.lastname: Nelson
authors.forename: R
authors.initials: R
authors.lastname: Monsell
authors.forename: N
authors.initials: N
authors.lastname: Siegel
authors.forename: B L
authors.initials: BL
authors.lastname: Haymore
authors.forename: R
authors.initials: R
authors.lastname: Leimgruber
authors.forename: J
authors.initials: J
authors.lastname: Feder
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects; D002460:Cell Line; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D002999:Clone Cells; D004261:DNA Replication / Q000187:drug effects; D015180:Electrophoresis, Gel, Two-Dimensional; D004730:Endothelium, Vascular / Q000378:metabolism; D006023:Glycoproteins / Q000302:isolation & purification*; D006168:Guinea Pigs; D006801:Humans; D007700:Kinetics; D008222:Lymphokines / Q000302:isolation & purification* / Q000494:pharmacology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification; D014357:Trypsin; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
chemical_list: D006023:Glycoproteins; D008222:Lymphokines; D010446:Peptide Fragments; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D014357:Trypsin