title: Treatment of human spermatozoa with an egg yolk medium can enhance the outcome of in vitro fertilization.
abstract: In at least 4 of 7 cases, fertilization of intact human oocytes was more successful when spermatozoa were pretreated with TEST yolk medium at 5 degrees C for 2 hours as compared with the standard treatment with Ham's F-10 only. Both pregnancies that were obtained after the transfer of the fertilized oocytes resulted from oocytes fertilized by TEST yolk-treated spermatozoa. No decrease in fertilization occurred in any of the cases after TEST yolk treatment. If these results hold true for a larger series of patients, it may be worthwhile for the standard IVF incubation system of spermatozoa to include TEST yolk.
authors.affiliation: Advanced Institute of Fertility, Sinai Samaritan Medical Center, Milwaukee, Wisconsin.
authors.forename: K P
authors.initials: KP
authors.lastname: Katayama
authors.forename: E
authors.initials: E
authors.lastname: Stehlik
authors.forename: M
authors.initials: M
authors.lastname: Roesler
authors.forename: R S
authors.initials: RS
authors.lastname: Jeyendran
authors.forename: W J
authors.initials: WJ
authors.lastname: Holmgren
authors.forename: L J
authors.initials: LJ
authors.lastname: Zaneveld
mesh_terms: D003470:Culture Media; D004530:Egg Yolk; D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D013094:Spermatozoa / Q000187:drug effects*
chemical_list: D003470:Culture Media