Ionic currents in neuroblastoma clone E-7 cells.
Ionic currents were studied in exponentially growing neuroblastoma cells (clone E-7) derived from mouse neuroblastoma C-1300 with the patch-clamp technique in the whole cell mode (Pflügers Arch., 391 (1981) 85-100). In differentiated cells, with one or several processes, an early inactivating inward current approximately equal to 50 microA/cm2 was observed in response to depolarizing steps from the holding potential -60 to -70 mV, which was insensitive to 2 microM tetrodotoxin but readily blocked by Co2+ (6 mM). This inward current was followed by a delayed outward current which was eliminated by 12 mM tetraethylammonium. In the undifferentiated cells, only delayed outward current was observed. It is suggested that, in differentiated cells both Ca2+ and delayed rectifier K+ channels exist, while only the latter are present in undifferentiated cells.
Vyklický
L
L
Michl
J
J
Vlachová
V
V
Vyklický
L
L
Vyskocil
F
F
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002999:Clone Cells; D003035:Cobalt / Q000494:pharmacology; D007473:Ion Channels / Q000502:physiology*; D008564:Membrane Potentials; D051379:Mice; D009447:Neuroblastoma; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D019789:Tetraethylammonium; D013757:Tetraethylammonium Compounds / Q000494:pharmacology; D013779:Tetrodotoxin / Q000494:pharmacology
D007473:Ion Channels; D013757:Tetraethylammonium Compounds; D003035:Cobalt; D013779:Tetrodotoxin; D019789:Tetraethylammonium; D012964:Sodium; D011188:Potassium; D002118:Calcium