Effect of atropine, PGF2 alpha and cimetidine on the beta-carotene induced cytoprotection in ethanol-treated rats.
The aim of the study was to evaluate the influence of atropine, PGF2 alpha and cimetidine on the gastric cytoprotective effect of beta-carotene. Mucosal damage was produced by intragastric (i.g.) addition of 96% ethanol in CFY-strain rats of both sexes weighing 180-220 g. Gastric cytoprotection caused by i. g. pretreatment with 1.0 mg/kg beta-carotene 30 minutes before ethanol administration, was observed after 1 hour. Atropine (0.5 mg/kg), cimetidine (50 mg/kg) and PGF2 alpha (200 micrograms/kg) were given intraperitoneally (i.p.) 30 minutes before ethanol administration with and without beta-carotene and the changes in the number and severity of the gastric ulcers were detected. PGF2 alpha did not influence the gastric cytoprotective effect of beta-carotene meanwhile it was inhibited by atropine and markedly by cimetidine. Deleterious effect of cimetidine on the beta-carotene-induced cytoprotection may be explained perhaps by the adverse effect of the two compounds on ATP-cAMP transformation hereby counteracting one another, but more data are needed to the better understanding of drug interactions relating to mucosal cytoprotection.
Garamszegi
M
M
First Department of Medicine, University Medical School Pécs, Hungary.
Jávor
T
T
Sütö
G
G
Vincze
A
A
Tóth
G
G
Mózsik
G
G
D000818:Animals; D001285:Atropine / Q000494:pharmacology*; D002338:Carotenoids / Q000037:antagonists & inhibitors / Q000627:therapeutic use*; D002927:Cimetidine / Q000494:pharmacology*; D015237:Dinoprost / Q000494:pharmacology*; D000431:Ethanol; D005260:Female; D005753:Gastric Mucosa / Q000166:cytology / Q000187:drug effects; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013276:Stomach Ulcer / Q000139:chemically induced / Q000517:prevention & control*; D019207:beta Carotene
D019207:beta Carotene; D002338:Carotenoids; D000431:Ethanol; D001285:Atropine; D002927:Cimetidine; D015237:Dinoprost