Evaluation and practical aspects of the use of a commercial DNA probe for detection of mycoplasma infections in cell cultures.
Cell cultures have been analyzed for mycoplasma infections by using a commercial DNA-probe based on rRNA genes from mycoplasmas. Both the original version, Mycoplasma T.C. Detection Kit, and the improved version of the kit, Mycoplasma T.C. II Rapid Detection System, were used. The sensitivities of the two tests were found to be adequate in most cases and the improved version of the kit was 10-100 times more sensitive than the original one. A batch variation was observed with the improved version, which is not satisfactory. This batch variation can, however, be checked and the performance of the method with a properly working lot was found to be good.
Johansson
K E
KE
National Veterinary Institute, Uppsala, Sweden.
Bölske
G
G
D000818:Animals; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003114:Colony-Forming Units Assay; D046508:Culture Techniques / Q000379:methods*; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D005798:Genes, Bacterial; D006801:Humans; D051379:Mice; D009174:Mycoplasma / Q000032:analysis* / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010954:Plasmacytoma; D012588:Scintillation Counting
D015342:DNA Probes; D004269:DNA, Bacterial; D004275:DNA, Ribosomal