Restriction endonuclease analysis of the DNA of Rickettsia prowazekii vaccine strain E and its revertant.
The DNA of Rickettsia prowazekii vaccine strain E was analysed by restriction analysis with 17 endonucleases in comparison with its virulent revertant - Evir and the virulent reference strain Breinl. The DNA of cloned and uncloned strains showed identical restriction endonuclease patterns. In spite of stable differences in virulence, strains E and Evir displayed a totally identical DNA cleavage pattern indicating the absence of marked structural differences between their genomes. On the other hand 9 endonucleases showed differences in the restrictograms of the DNA strain Breinl as compared with strains E and Evir.
Balaeva
N M
NM
N. F. Gamaleya Research Institute of Epidemiology and Microbiology, U.S.S.R., Academy of Medical Sciences, Moscow.
Rydkina
E B
EB
Artemiev
M I
MI
Ignatovich
V F
VF
D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D004996:Ethidium; D014644:Genetic Variation / Q000235:genetics; D012283:Rickettsia prowazekii / Q000235:genetics*; D014774:Virulence / Q000235:genetics
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes; D004996:Ethidium