New classes of common fragile sites induced by 5-azacytidine and bromodeoxyuridine.
Two new classes of common fragile site seen in chromosomes from blood lymphocyte cultures are reported. The first class is induced in bands 1q42 and 19q13 by 5-azacytidine (5-AZA). Maximum induction of these fragile sites occurs when the 5-AZA is added 5-8h prior to harvest. The second class is induced in bands 6q13, 9p21, and 10q21 by bromodeoxyuridine (BrdU). In this instance maximum induction occurred if the BrdU was added 4-6h prior to harvest. The known fragile sites, both rare and common, are summarised.
Sutherland
G R
GR
Parslow
M I
MI
Baker
E
E
D000328:Adult; D001374:Azacitidine / Q000494:pharmacology*; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D002871:Chromosome Banding; D043283:Chromosome Fragile Sites; D002873:Chromosome Fragility; D002903:Chromosomes, Human, 19-20; D002906:Chromosomes, Human, 6-12 and X; D006801:Humans; D007231:Infant, Newborn; D007621:Karyotyping
D001973:Bromodeoxyuridine; D001374:Azacitidine