Superinduction of the human gene encoding low density lipoprotein receptor.
Expression of human LDL receptor mRNA and protein is induced in human glioblastoma-astrocytoma cells upon transfer into lipoprotein-deficient medium, a mode of induction likely to be physiological. The presence of cycloheximide (CHX) leads to up to 7.5-fold superinduction of LDL receptor mRNA within 4 hr and, upon removal of this inhibitor, to superinduction of LDL receptor protein as well. The extent of superinduction of LDL receptor mRNA reaches over 40-fold beyond the level expressed in medium containing regular serum. Despite its extensive superinduction, LDL receptor mRNA decays rapidly in the presence of CHX. Stabilization of LDL receptor mRNA is thus unlikely to account for the observed superinduction. These results show that normally the LDL receptor gene is expressed to only a small fraction of its full potential.
Leitersdorf
E
E
Department of Internal Medicine B, Hadassah University Hospital, Jerusalem, Israel.
Banai
Y
Y
Friedman
G
G
Kaempfer
R
R
D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D005786:Gene Expression Regulation; D005796:Genes / Q000187:drug effects; D006801:Humans; D006822:Hybrid Cells / Q000378:metabolism; D015151:Immunoblotting; D007700:Kinetics; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics; D011973:Receptors, LDL / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D015854:Up-Regulation
D012333:RNA, Messenger; D011973:Receptors, LDL; D003513:Cycloheximide