title: [Specificity of enzymes of biosynthesis of Salmonella anatum O-antigen for polyprenyl derivatives of different chain length and saturation].
abstract: Polyprenyl phosphates of different structure were prepared and their ability to serve as sugar acceptors in the biosynthesis of O-specific polysaccharides of Salmonella anatum was investigated. It was demonstrated that C30-C80-polyprenyl phosphates with unsaturated alpha-isoprene unit were as active as natural acceptor (undecaprenyl phosphate) in this enzymic system. C15- and C100-polyprenyl phosphates of this series were less effective in O-antigen repeating unit formation. Citronellyl and dolichyl phosphates (derivatives of C10- and C105-polyprenols, respectively, with saturated alpha-isoprene unit) were poor substrates. For polymerization of repeating units, the polyprenol chain-length is of utmost importance: its shortening results in a marked drop in the efficiency of respective compounds as substrates.
authors.forename: N A
authors.initials: NA
authors.lastname: Kalinchuk
authors.forename: L L
authors.initials: LL
authors.lastname: Danilov
authors.forename: T N
authors.initials: TN
authors.lastname: Druzhinina
authors.forename: V N
authors.initials: VN
authors.lastname: Shibaev
authors.forename: N K
authors.initials: NK
authors.lastname: Kochetkov
mesh_terms: D000942:Antigens, Bacterial; D005700:Galactosyltransferases; D019081:O Antigens; D011105:Polyisoprenyl Phosphate Sugars / Q000138:chemical synthesis*; D011106:Polyisoprenyl Phosphates / Q000138:chemical synthesis; D011108:Polymers; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D012475:Salmonella / Q000276:immunology*; D013379:Substrate Specificity
chemical_list: D000942:Antigens, Bacterial; D019081:O Antigens; D011105:Polyisoprenyl Phosphate Sugars; D011106:Polyisoprenyl Phosphates; D011108:Polymers; D011135:Polysaccharides, Bacterial; D005700:Galactosyltransferases
vernacular_title: Spetsifichnost' fermentov biosinteza O-antigena Salmonella anatum k proizvodnym poliprenolov raznoĭ dliny tsepi i nasyshchennosti.