Murinoglobulin, a novel protease inhibitor from murine plasma. Isolation, characterization, and comparison with murine alpha-macroglobulin and human alpha-2-macroglobulin.
Two glycoproteins having trypsin-protein esterase activity were purified to apparent homogeneity from murine plasma. One was alpha-macroglobulin, a homologue of human alpha-2-macroglobulin, while the other, tentatively named murinoglobulin, did not correspond to any of the known plasma protease inhibitors that have been well characterized in men or other mammals. Murinoglobulin contained about 7.6% carbohydrate and was composed of a single-polypeptide chain of Mr = 180,000 as judged by the equilibrium sedimentation analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions. Murinoglobulin did not cross-react immunologically with mouse alpha-macroglobulin nor with human alpha-2-macroglobulin. Protease-inhibiting properties of murinoglobulin were compared with those of mouse alpha-macroglobulin and human alpha-2-macroglobulin. All the three proteins inhibited trypsin, papain, and thermolysin, although they differed considerably in both the degree of inhibition and the binding stoichiometry of protease-inhibitor complexes. The two macroglobulins inhibited pepsin at pH 5.5, whereas murinoglobulin was inactivated at this pH. Murinoglobulin was more sensitive to methylamine than the two macroglobulins. No protein corresponding to murinoglobulin was detected in human plasma.
Saito
A
A
Sinohara
H
H
D000818:Animals; D004950:Esterases / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008297:Male; D008744:Methylamines / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D011480:Protease Inhibitors / Q000302:isolation & purification*; D012712:Serum Globulins / Q000302:isolation & purification*; D013438:Sulfhydryl Compounds / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism; D014360:Trypsin Inhibitor, Kunitz Soybean / Q000494:pharmacology; D000511:alpha-Macroglobulins / Q000032:analysis*
D008744:Methylamines; D011480:Protease Inhibitors; D012712:Serum Globulins; D013438:Sulfhydryl Compounds; D000511:alpha-Macroglobulins; C044177:murinoglobulin; D014360:Trypsin Inhibitor, Kunitz Soybean; C027451:methylamine; D004950:Esterases; D014357:Trypsin