Membrane disordering by anesthetic drugs: relationship to synaptosomal sodium and calcium fluxes.
The effects of membrane perturbants (ethanol, pentobarbital, chloroform, diethylether, phenytoin, cis-vaccenic acid methylester, and cis-vaccenoyl alcohol) on the lipid order of mouse brain synaptic plasma membranes (SPM) were tested by fluorescence polarization using 1,6-diphenyl-1,3,5-hexatriene (DPH) as a probe of the membrane core and 1-[4-(trimethylammonium)phenyl]-6-phenyl-1,3,5-hexatriene (TMA-DPH) as a probe of the membrane surface. The compounds decreased the fluorescence polarization of both probes, indicating that they disordered the membrane lipids. The decrease in polarization was, however, greater for DPH than for TMA-DPH, suggesting a greater effect on the membrane core than on the membrane surface. The voltage-dependent uptake of 24Na and 45Ca was studied in isolated mouse brain synaptosomes as a measure of membrane function. All of the compounds inhibited sodium influx, and their potencies for decreasing sodium uptake and fluorescence polarization of DPH were linearly correlated (r = 0.91). The relationship between changes in sodium influx and TMA-DPH polarization was less consistent (r = 0.66). Synaptosomal calcium uptake was inhibited by most, but not all, of the perturbants, but this inhibition was poorly correlated with changes in fluorescence polarization of DPH (r = 0.36) or TMA-DPH (r = 0.26). These results indicate that the function of synaptic sodium channels is correlated with lipid order in the hydrophobic core of the membrane and that the inhibitory effects of intoxicant-anesthetic drugs on neuronal sodium fluxes may be the result of their capacity to disorder these lipids. In contrast, the effects of drugs on voltage-dependent calcium channels were not clearly related to the capacity of these agents to disorder membrane lipids.
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D000777:Anesthetics / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004161:Diphenylhexatriene / Q000031:analogs & derivatives; D005229:Fatty Acids, Monounsaturated; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology; D005454:Fluorescence Polarization; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects; D008563:Membrane Lipids / Q000502:physiology*; D051379:Mice; D008811:Mice, Inbred DBA; D009829:Oleic Acids / Q000494:pharmacology; D012964:Sodium / Q000378:metabolism*; D013570:Synaptic Membranes / Q000187:drug effects* / Q000502:physiology; D014783:Viscosity
D000777:Anesthetics; D005229:Fatty Acids, Monounsaturated; D005231:Fatty Acids, Unsaturated; D007473:Ion Channels; D008563:Membrane Lipids; D009829:Oleic Acids; D004161:Diphenylhexatriene; C044856:11-octadecenoyl alcohol; C040123:methyl vaccenate; C033628:1-(4-(trimethylamino)phenyl)-6-phenylhexa-1,3,5-triene; D012964:Sodium; D002118:Calcium