[Mycoplasma contamination of cell cultures: methods for its detection and the possible routes of Mycoplasma infection spread].
83 continuous cell lines were screened for mycoplasma contamination by three methods: microbiological seeding for enriched media, staining with Hoechst-33258 stain, and autoradiography with 3H-thymidine incorporation. It has been shown that combination of different methods is necessary for the strict control of mycoplasma-contamination in cell cultures. Simultaneous manipulation with mycoplasma infected and pure cell lines leads to cross-contamination in 2-3 passages. Precautions are described to preclude mycoplasma-contamination during prolonged cell cultivation.
Smirnova
T D
TD
Fridlianskaia
I I
II
D000818:Animals; D001345:Autoradiography / Q000379:methods; D001431:Bacteriological Techniques; D002460:Cell Line; D002478:Cells, Cultured / Q000382:microbiology*; D009175:Mycoplasma Infections / Q000382:microbiology* / Q000517:prevention & control / Q000635:transmission; D013194:Staining and Labeling / Q000379:methods
Kontaminatsiia kletochnykh kul'tur mikoplazmami: metody obnaruzheniia i vozmozhnye puti rasprostraneniia mikoplazma-infektsii.