title: Differences in the fine specificity of anti-Ro (SS-A) in relation to the presence of other precipitating autoantibodies.
abstract: In sera from patients with systemic lupus erythematosus or Sjögren's syndrome, we determined the fraction of antibody that remained reactive with human Ro (SS-A) after absorption with bovine spleen extract, and the reactivity with the 60-kd and 54-kd red blood cell Ro (SS-A) bands by Western blot. Of the 3 groups of sera studied, those containing anti-Ro (SS-A) alone had the highest degree of reactivity with human Ro (SS-A) after absorption with bovine spleen extract, followed, in descending order, by sera containing anti-Ro (SS-A) and anti-La (SS-B), and sera containing anti-Ro (SS-A) and anti-nuclear RNP. The groups of sera could be distinguished on this basis. Sera with anti-Ro (SS-A) and anti-nuclear RNP could also be distinguished from the other 2 types of sera by their uniform and preferential reactivity with the 60-kd red blood cell Ro (SS-A), by Western blot analysis. These findings indicate that there are both qualitative and quantitative differences, associated with the presence of other autoantibodies, in the fine specificity of anti-Ro (SS-A) sera.
authors.affiliation: Arthritis and Immunology Laboratory, Oklahoma Medical Research Foundation, Oklahoma City 73104.
authors.forename: M D
authors.initials: MD
authors.lastname: Rader
authors.forename: C
authors.initials: C
authors.lastname: Codding
authors.forename: M
authors.initials: M
authors.lastname: Reichlin
mesh_terms: D000818:Animals; D000974:Antibodies, Antinuclear / Q000276:immunology*; D000918:Antibody Specificity; D001323:Autoantibodies / Q000032:analysis*; D001324:Autoantigens / Q000276:immunology; D015153:Blotting, Western; D002417:Cattle; D002467:Cell Nucleus / Q000276:immunology; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D011233:Precipitin Tests; D012261:Ribonucleoproteins; D012859:Sjogren's Syndrome / Q000276:immunology; D013154:Spleen / Q000032:analysis; D014020:Tissue Extracts / Q000276:immunology; D055517:snRNP Core Proteins; D000097585:SS-B Antigen
chemical_list: D000974:Antibodies, Antinuclear; D001323:Autoantibodies; D001324:Autoantigens; D012261:Ribonucleoproteins; D014020:Tissue Extracts; D055517:snRNP Core Proteins; D000097585:SS-B Antigen; C035356:SS-A antibodies