Atypical human rotaviruses in the G.D.R.
Following the aetiologic agent of diarrhoea in children aged one to three years we found in the stools a large number of rotaviruses by electron microscopy (EM), although ELISA was negative; the sensitivity of ELISA had been confirmed in previous investigations. In 141 children with diarrhoea 10 conventional (group A) and 21 antigenically distinct rotaviruses were detected. The presence of atypical rotaviruses could be confirmed by electrophoretic analysis of their RNA migration profiles. The electropherotype pattern corresponded to the group C rotaviruses reported by others.
Böthig
B
B
Schulze
P
P
Schreier
E
E
Diedrich
S
S
Michel
S
S
D000956:Antigens, Viral / Q000302:isolation & purification; D002675:Child, Preschool; D003967:Diarrhea / Q000276:immunology / Q000382:microbiology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005859:Germany, East; D006801:Humans; D007223:Infant; D008854:Microscopy, Electron; D012367:RNA, Viral / Q000302:isolation & purification; D012401:Rotavirus / Q000276:immunology / Q000302:isolation & purification* / Q000648:ultrastructure
D000956:Antigens, Viral; D012367:RNA, Viral