[Analysis of T-cell subpopulations and expression patterns of lymphocytic activation markers in patients in the early phase following kidney transplantation using laser flow cytometry].
In 10 patients after kidney transplantation the T cell subpopulations (CD4, CD8) and the number of activated lymphocytes (IL-2R and transferrin receptor+, 4F2+, HLA-DR+, CD3+) in the peripheral blood were determined. In 9 out of 9 cases with rejection a clear immune activation could observed. In patients with CMV-associated rejection crisis the CD4/CD8 ratio was significantly higher compared with patients with rejection crisis but without CMV infection. In all patients with CMV infection (n = 5) an increase of activated lymphocytes was observed. In a clinical-immunological monitoring the dynamics of the CD4/CD8 ratio, the expression of activation marker (especially CD3+, HLA-DR+ and 4F2+) and the absolute number of lymphocyte subpopulations should included.
Kaden
J
J
Abteilung für Experimentelle Organtransplantation der Urologischen Klinik des Bereiches Medizin Humboldt-Universität zu Berlin.
Falck
P
P
Eichler
C
C
Strobelt
V
V
May
G
G
Volk
H D
HD
D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D003586:Cytomegalovirus Infections / Q000276:immunology; D005260:Female; D006084:Graft Rejection / Q000276:immunology; D006801:Humans; D007676:Kidney Failure, Chronic / Q000601:surgery*; D016030:Kidney Transplantation / Q000276:immunology*; D007958:Leukocyte Count; D008213:Lymphocyte Activation / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011183:Postoperative Complications / Q000175:diagnosis*; D013601:T-Lymphocytes / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
Analyse von T-Zellsubpopulationen und Expressionsmustern lymphozytärer Aktivierungsmarker bei Patienten in der Frühphase nach Nierentransplantation mittels Laser-Durchflusszytometrie.