title: Regional distribution of neuropeptide Y mRNA in postmortem human brain.
abstract: The distribution of messenger RNA encoding neuropeptide Y (NPY) was studied in 11 different postmortem human brain regions using in situ hybridization histochemistry, and RNA blot analysis. In situ hybridization data revealed that the highest numerical density of labeled cells corresponded to neurons in accumbens area, caudate nucleus, putamen, and substantia innominata. Significantly fewer NPY mRNA-containing neurons were found in frontal and parietal cortex, amygdaloid body and dentate gyrus. No NPY mRNA-containing cells were found in substantia nigra. NPY mRNA-positive neurons from all regions studied showed relatively similar labeling, as revealed by computerized image analysis. Blot analysis showed an approximately 0.8 kb NPY mRNA in all brain regions studied, except in substantia nigra and cerebellum. Densitometric scanning of the autoradiograms revealed levels of NPY mRNA in the following order: putamen greater than caudate nucleus greater than frontal cortex (Brodmann areas 4 and 6) greater than temporal cortex (Brodmann area 38) greater than parietal cortex (Brodmann areas 5 and 7) greater than frontal cortex (Brodmann area 11). Hence, although NPY mRNA is widely distributed in neurons of the human brain large regional variation exists, with the highest expression in accumbens area and parts of the basal ganglia.
authors.affiliation: Department of Medical Chemistry, Karolinska Institute, Stockholm, Sweden.
authors.forename: S
authors.initials: S
authors.lastname: Brené
authors.forename: N
authors.initials: N
authors.lastname: Lindefors
authors.forename: J
authors.initials: J
authors.lastname: Kopp
authors.forename: G
authors.initials: G
authors.lastname: Sedvall
authors.forename: H
authors.initials: H
authors.lastname: Persson
mesh_terms: D000368:Aged; D001921:Brain / Q000378:metabolism*; D006801:Humans; D008875:Middle Aged; D009478:Neuropeptide Y / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*
chemical_list: D009478:Neuropeptide Y; D012333:RNA, Messenger