Effect of immunotherapy on the serum concentrations of allergen-specific IgG antibodies in dog sera.
An ELISA assay which uses horseradish peroxidase conjugated anti-canine IgG and polystyrene microtiter wells for detection of allergen-specific IgG in the serum of dogs is described. Individual allergen blanks were used to account for the variable nonspecific binding among various allergens, and the results observed in milliunits of absorbance were normalized using four reference sera. The coefficients of variation for the intraassay and interassay variability ranged from 1.34 to 12.50% and 4.62 to 9.77%, respectively. The relationship between ELISA results and serum concentrations of allergen-specific IgG was quantified. IgG antibodies with specificity for various allergens were found in the majority of non-atopic individuals and in all atopic subjects. Specific immunotherapy resulted in a rise in the serum concentration of allergen-specific IgG.
Hites
M J
MJ
Iatric Corporation, Tempe, AZ 85282.
Kleinbeck
M L
ML
Loker
J L
JL
Lee
K W
KW
D000485:Allergens / Q000276:immunology; D000818:Animals; D000918:Antibody Specificity; D003888:Desensitization, Immunologic / Q000662:veterinary*; D004283:Dog Diseases / Q000276:immunology / Q000628:therapy*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D007074:Immunoglobulin G / Q000032:analysis / Q000378:metabolism*; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology / Q000628:therapy / Q000662:veterinary*
D000485:Allergens; D007074:Immunoglobulin G