The use of hyaluronic acid for sperm selection--evaluation of sperm head ultrastructure.
The percentage of normal spermatozoal plasma membranes and acrosomes was evaluated by electron microscopy before and after a two-step semen washing and swim-up into culture medium containing either bovine serum albumin or hyaluronic acid. Both techniques of sperm selection increased the concentration of motile spermatozoa when compared with the fresh ejaculate (P less than .001). The percentage of sperm with ultrastructurally intact plasma membranes and acrosomes was increased after both separation procedures in comparison with the original ejaculate (P less than .01). In addition, both techniques of sperm selection significantly reduced the content of sample debris, granulocytes, and nonviable spermatozoa (P less than .01).
Bower
L
L
Department of Obstetrics and Gynecology, University of California Irvine, Orange.
Serafini
P
P
Wikland
M
M
Moyer
D
D
D000177:Acrosome / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects; D003470:Culture Media; D005260:Female; D006801:Humans; D006820:Hyaluronic Acid / Q000494:pharmacology*; D008297:Male; D008854:Microscopy, Electron; D013077:Sperm Head / Q000187:drug effects*; D013081:Sperm Motility / Q000187:drug effects*; D013084:Sperm-Ovum Interactions / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects*
D003470:Culture Media; D006820:Hyaluronic Acid