Identification and characterization of a polyadenylated small RNA (s-poly A+ RNA) in dinoflagellates.
A 104 nucleotide-long small RNA, referred to as s-poly A+ RNA, containing 30 adenosine residues on its 3' -end was found in dinoflagellates, purified and its nucleotide sequence was determined. The sequence is: (sequence text) The polyadenylation signal AAUAAA was not found in this RNA; this result indicates that the 30 nucleotide-long poly A on the 3' -end is either coded for by this gene, or the poly A chain is added on this small RNA by a mechanism different from that for polyadenylation of messenger RNAs. Two polyadenylated small RNAs identified previously were implicated in differentiation of chicken heart muscle cells (Deshpande, A. K., Jakowlew, S. B., Arnold, H., Crawford, P. A. and Siddiqui, M. A. Q. (1977) J. Biol. Chem. 252, 6521-6527), and in brain specific mRNA transcription (Sutcliffe, J. G., Milner, R. J., Gottesfeld, J. M. and Lerner, R. A. (1984) Nature 309, 237-241). This RNA is the first polyadenylated small RNA to be sequenced.
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D000818:Animals; D001483:Base Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D004141:Dinoflagellida / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D011061:Poly A / Q000302:isolation & purification*; D012313:RNA / Q000302:isolation & purification*; D012333:RNA, Messenger; D012342:RNA, Small Nuclear
D012333:RNA, Messenger; D012342:RNA, Small Nuclear; D011061:Poly A; D012313:RNA