Afrikander cattle congenital goiter: size heterogeneity in thyroglobulin mRNA.
The mRNA coding for thyroglobulin in cattle homozygous for an autosomal recessive defect of thyroglobulin synthesis was investigated using a recombinant plasmid containing bovine mRNA coding sequence. Total RNA preparations from goiter contained one third of the thyroglobulin mRNA sequences found in normal thyroid tissue. This mRNA was not translated into thyroglobulin by Xenopus oocytes. Northern transfer analysis revealed both a normal sized and a smaller thyroglobulin mRNA in the goiter.
Ricketts
M H
MH
Vandenplas
S
S
van der Walt
M
M
van Jaarsveld
P P
PP
Bester
A J
AJ
Boyd
C D
CD
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000235:genetics*; D004247:DNA / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006042:Goiter / Q000235:genetics / Q000662:veterinary*; D009693:Nucleic Acid Hybridization; D009865:Oocytes / Q000378:metabolism; D011061:Poly A / Q000378:metabolism; D014176:Protein Biosynthesis; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis*; D013954:Thyroglobulin / Q000235:genetics*; D013961:Thyroid Gland / Q000032:analysis; D014981:Xenopus
D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004247:DNA; D013954:Thyroglobulin