Blot analyses of glycoconjugates: enzyme-hydrazide--a novel reagent for the detection of aldehydes.
A procedure for the general staining of glycoproteins and other glycoconjugates on protein blots has been developed. Aldehydes are formed on the sugars of glycoconjugates by periodate oxidation which then react with hydrazide groups of enzyme-hydrazides, a novel reagent designed for aldehyde detection. The bound enzyme-hydrazide is demonstrated histochemically. The new assay is advantageous over periodic-acid Schiff staining of gels as its reagents and signals are stable and the process is simple and expedient, and provides greater sensitivity.
Gershoni
J M
JM
Bayer
E A
EA
Wilchek
M
M
D000447:Aldehydes / Q000032:analysis*; D000469:Alkaline Phosphatase; D001212:Asialoglycoproteins; D004591:Electrophoresis, Polyacrylamide Gel; D060748:Fetuins; D006021:Glycophorins / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis*; D006735:Horseradish Peroxidase; D006801:Humans; D006834:Hydrazines; D007202:Indicators and Reagents; D010544:Peroxidases; D013194:Staining and Labeling; D000509:alpha-Fetoproteins / Q000032:analysis
D000447:Aldehydes; D001212:Asialoglycoproteins; D060748:Fetuins; D006021:Glycophorins; D006023:Glycoproteins; D006834:Hydrazines; D007202:Indicators and Reagents; D000509:alpha-Fetoproteins; C017029:asialofetuin; D006735:Horseradish Peroxidase; D010544:Peroxidases; D000469:Alkaline Phosphatase