Morphological and functional characteristics of myocytes isolated from human left ventricular aneurysms.
Isolated single myocytes were prepared from myocardium of developing ventricular aneurysms and from myocardium within the scar of chronic ventricular aneurysms. The morphology and function of the individual cells were compared. The cells from developing aneurysms were rod-shaped, with a distinct sarcomeric structure, but did not contract even in the presence of high calcium concentrations. The sarcomere length was significantly higher than that of cells from chronic aneurysms and approached the theoretical point at which no contraction can occur. Cells from chronic aneurysms were either rod-shaped and contractile, or rounded due to hypercontracture of the myofilaments. Electron microscopy of cells from developing aneurysms confirmed the presence of elongated sarcomeres, a loss of the actin-myosin interdigitation, and damage to the contractile proteins which was particularly evident in the thin filaments. Cells with similar characteristics have also been isolated from a ruptured, ischaemic papillary muscle. These changes, which are due either to ischaemia or to overstretching of cells, may account for the weakness of the wall of developing aneurysms and be a cause of rupture or enlargement.
Harding
S E
SE
National Heart and Lung Institute, London, U.K.
Vescovo
G
G
Jones
S M
SM
Bennett
G
G
Yacoub
M
M
Poole-Wilson
P A
PA
D000328:Adult; D000368:Aged; D002469:Cell Separation; D005260:Female; D006321:Heart / Q000503:physiopathology; D006322:Heart Aneurysm / Q000473:pathology*; D006352:Heart Ventricles / Q000648:ultrastructure; D006801:Humans; D008297:Male; D008875:Middle Aged; D009119:Muscle Contraction; D009206:Myocardium / Q000648:ultrastructure*; D012518:Sarcomeres / Q000473:pathology