Structure and functional expression of a cloned Xenopus thyroid hormone receptor.
A clone corresponding to a thyroid hormone receptor was isolated from a Xenopus laevis cDNA library prepared from folliculated oocytes. The cDNA encodes a protein of 418 amino acid residues with a domain structure, including a putative DNA binding region with two zinc fingers, similar to other members of the v-erbA-related superfamily of receptors. The encoded protein resembles the TR alpha 1-type receptor of the rat. When expressed in COS cells the protein product binds triiodothyronine with a Kd of 0.12 nM. The receptor mediates thyroid-hormone-inducible expression of a reporter gene which includes a thyroid hormone response element in its upstream region.
Brooks
A R
AR
Department of Biological Sciences, University of Warwick, Coventry, UK.
Sweeney
G
G
Old
R W
RW
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D015870:Gene Expression; D015723:Gene Library; D005796:Genes; D007700:Kinetics; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism; D014176:Protein Biosynthesis; D011487:Protein Conformation; D011988:Receptors, Thyroid Hormone / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014162:Transfection; D014982:Xenopus laevis
D011988:Receptors, Thyroid Hormone; D011994:Recombinant Proteins