Characterization of Hodgkin's disease derived cell line HDLM-2.
The cell line HDLM-2 was established from the pleural effusion of a patient with Hodgkin's disease. Here, we describe the morphological, cytochemical, enzymological, immunological, molecular biological, and functional characteristics of the cell line. The results of this multiparameter profile show that HDLM-2 is different from other well-studied leukemia-lymphoma cell lines including other Hodgkin's disease derived cell lines. HDLM-2 cultures contain mainly mono- or binucleated cells, but also prominent giant cells with two to ten nuclei. HDLM-2 cells do not express an immunophenotype characteristic of a given cell lineage. However, the cells are positive for Ki-1, HeFi-1, Leu-M1, Tac, and HLA class II markers. Cytochemical, enzymological, and functional data are equally inconclusive, but are definitely not compatible with a monocyte/macrophage profile. Analysis of the gene status documents that T-cell receptor beta- and gamma-chain genes are rearranged while immunoglobulin heavy chain genes are in germline configuration. The combined results indicate a T-cell origin of HDLM-2 cells. The evidence available from this and other established Hodgkin's disease derived cell lines suggests a lymphoid origin of Hodgkin and Reed-Sternberg cells.
Drexler
H G
HG
Department of Haematology, Royal Free Hospital, School of Medicine, London, Great Britain.
Gignac
S M
SM
Hoffbrand
A V
AV
Leber
B F
BF
Norton
J
J
Lok
M S
MS
Minowada
J
J
D000818:Animals; D014408:Biomarkers, Tumor; D006651:Histocytochemistry; D006689:Hodgkin Disease / Q000276:immunology / Q000473:pathology*; D006801:Humans; D007700:Kinetics; D014407:Tumor Cells, Cultured / Q000276:immunology*
D014408:Biomarkers, Tumor