[Effect of hemodialysate and its peptide fragments on stromal cells and heme synthesis in bone marrow culture and the activity of selected enzymes and GSH level in human erythrocytes. III. Effect on G-6-PD in erythrocytes of healthy persons and those with terminal renal failure].
It has been found that erythrocytes of hemodialysed patients show significantly increased glucose-6-phosphate dehydrogenase (G-6-PD, E.C. 1.1.1.49) activity when compared with that in healthy subjects. Activity of that enzyme in erythrocytes from patients treated with hemodialyses incubated in 3 hours with hemodialysate (HD) or its peptide fractions (PF) showed no statistical difference when compared with controls. Similar effect of HD or PF on activity of the enzyme studied has been noted in erythrocytes from healthy subjects except fraction II having concentration equal to 14 micrograms/ml calculated against the protein content. This last fraction caused significant inhibition of the enzyme activity.
Smoleński
O
O
D002478:Cells, Cultured; D003470:Culture Media; D015314:Dialysis Solutions / Q000494:pharmacology*; D004789:Enzyme Activation; D004912:Erythrocytes / Q000201:enzymology*; D005954:Glucosephosphate Dehydrogenase / Q000097:blood*; D015312:Hemodialysis Solutions / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000628:therapy; D010446:Peptide Fragments / Q000494:pharmacology*; D012016:Reference Values; D006435:Renal Dialysis
D003470:Culture Media; D015314:Dialysis Solutions; D015312:Hemodialysis Solutions; D010446:Peptide Fragments; D005954:Glucosephosphate Dehydrogenase
Wpływ hemodializatu i jego frakcji peptydowych na komórki podścieliska i synteze hemu w hodowli szpiku oraz na aktywność wybranych enzymów i stezenie GSH w krwinkach czerwonych człowieka. Cześć III: Wpływ na aktywność G-6-PD w erytrocytach ludzi zdrowych i chorych w schyłkowej niewydolności nerek.