title: Hydroxyl free radical adduct of deoxyguanosine: sensitive detection and mechanisms of formation.
abstract: DNA or 2-deoxyguanosine reacts with hydroxyl free radical to form 8-hydroxy-deoxyguanosine (8-OH-dG). We found that 8-OH-dG can be effectively separated from deoxyguanosine by high pressure liquid chromatography and very sensitively detected using electrochemical detection. The sensitivity of electrochemical detection is about one-thousand fold enhanced over optical detection. Utilizing deoxyguanosine in bicarbonate buffer it was found that ferrous ion, but not ferric ion, was effective in forming 8-OH-dG. The hydroxyl free radical scavenging agents, thiourea and ethanol, were very effective in quenching Fe(11) mediated 8-OH-dG formation, but superoxide dismutase had very little effect.
authors.affiliation: Oklahoma Medical Research Foundation, Biomembrane Research Program, Oklahoma City 73104.
authors.forename: R A
authors.initials: RA
authors.lastname: Floyd
authors.forename: J J
authors.initials: JJ
authors.lastname: Watson
authors.forename: P K
authors.initials: PK
authors.lastname: Wong
authors.forename: D H
authors.initials: DH
authors.lastname: Altmiller
authors.forename: R C
authors.initials: RC
authors.lastname: Rickard
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D002851:Chromatography, High Pressure Liquid; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism*; D004563:Electrochemistry; D000431:Ethanol / Q000494:pharmacology; D016166:Free Radical Scavengers; D005609:Free Radicals; D017665:Hydroxyl Radical / Q000032:analysis / Q000378:metabolism*; D013890:Thiourea / Q000494:pharmacology
chemical_list: D016166:Free Radical Scavengers; D005609:Free Radicals; D017665:Hydroxyl Radical; D000431:Ethanol; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; D013890:Thiourea