1,25-Dihydroxyvitamin D3-induced calcium-binding protein: localization in organ-cultured embryonic chick duodenum.
1,25-Dihydroxyvitamin D3 (1,25(OH)2D3) induces de novo biosynthesis of a specific calcium-binding protein (CaBP) in embryonic chick duodenum in organ culture. Using a highly sensitive and specific, peroxidase-antiperoxidase immunocytochemical procedure, 1,25(OH)2D3-induced CaBP in the organ-cultured duodenum was found only in the cytoplasm of absorptive cells, corresponding to its localization in rachitic chick duodenal cells after a single injection of 1,25(OH)2D3 in vivo. This observation, along with evidence correlating CaBP with calcium transport, strongly supports the use of the embryonic chick duodenal organ culture system as a physiologically relevant model of the vitamin D-dependent calcium absorptive mechanism.
Corradino
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Taylor
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D000042:Absorption; D000818:Animals; D017317:Annexin A6; D001692:Biological Transport; D002117:Calcitriol / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000096:biosynthesis / Q000378:metabolism*; D002642:Chick Embryo; D003593:Cytoplasm / Q000378:metabolism; D004386:Duodenum / Q000196:embryology / Q000378:metabolism*; D006651:Histocytochemistry; D007473:Ion Channels / Q000378:metabolism; D009924:Organ Culture Techniques
D017317:Annexin A6; D002135:Calcium-Binding Proteins; D007473:Ion Channels; D002117:Calcitriol