An improved method to determine cell viability by simultaneous staining with fluorescein diacetate-propidium iodide.
A rapid, simultaneous double-staining procedure using fluorescein diacetate (FDA) and propidium iodide (PI) is described for use in the determination of cell viability in cell suspension. Air-dried slide preparations can be made from the cell suspensions so that an accurate estimate of the viability of the cells in the original suspension can be made up to 1 week later. Viable cells fluoresce bright green, while nonviable cells are bright red. Furthermore, when FDA-PI staining is compared to trypan blue dye exclusion as a method to determine cell viability, FDA-PI is found to be more consistent over prolonged periods of exposure to the dyes. Therefore, double staining with FDA-PI is a rapid, convenient, and reliable method to determine cell viability.
Jones
K H
KH
Senft
J A
JA
D000818:Animals; D002452:Cell Count; D002470:Cell Survival; D005452:Fluoresceins; D006651:Histocytochemistry / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence / Q000379:methods; D010617:Phenanthridines; D011419:Propidium; D013154:Spleen / Q000166:cytology; D013194:Staining and Labeling / Q000379:methods*; D014343:Trypan Blue
D005452:Fluoresceins; D010617:Phenanthridines; D011419:Propidium; D014343:Trypan Blue; C018506:diacetylfluorescein