Quantification of unscheduled DNA synthesis by a whole cell counting method.
A procedure was developed for the quantification of the autoradiographic assay for unscheduled DNA synthesis. Relative to commonly used practices for grain counting, this procedure provides a more accurate net nuclear grain count by eliminating the subjectivity currently associated with selection of the areas to be counted for the cytoplasmic background count. Briefly, the object area and aperture area modes of an ARTEK 880 colony counter are used to collect values for the total number of silver grains over a particular cell (nuclear and cytoplasmic counts), as well as for the nuclear and cytoplasmic areas. These values are then employed in a short algorithm to determine the net nuclear grain count. This new method provides greater sensitivity for defining weak UDS responses and the data collected readily lends itself to statistical analysis.
Hill
L E
LE
Toxicology Division, Lilly Research Laboratories, Eli Lilly and Company, Greenfield, IN 46140.
Yount
D J
DJ
Garriott
M L
ML
Tamura
R N
RN
Probst
G S
GS
D015073:2-Acetylaminofluorene / Q000494:pharmacology; D000818:Animals; D001345:Autoradiography; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis; D004260:DNA Repair; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D008099:Liver / Q000166:cytology; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D009152:Mutagenicity Tests / Q000592:standards*; D051381:Rats; D011916:Rats, Inbred F344; D013223:Statistics as Topic
D008769:Methylnitronitrosoguanidine; D004247:DNA; D015073:2-Acetylaminofluorene; D004121:Dimethyl Sulfoxide