Comparison of improved precipitation methods for quantification of high-density lipoprotein cholesterol.
We compared the standard Lipid Research Clinics heparin-Mn2+ (46 mmol/L) method and five improved precipitation methods for quantification of high-density lipoprotein (HDL) cholesterol. Three of these methods--a dextran sulfate-Mg2+ procedure, reported as a Selected Method, a modified heparin-Mn2+ (92 mmol/L) method, and a modified phosphotungstate-Mg2+ procedure--all gave similar results. Three other methods--the standard heparin-Mn2+ (46 mmol/L) method and two polyethylene glycol methods (75 g/L or pH 10 reagent at 100 g/L final concentrations)--gave slightly higher values for HDL cholesterol. Addition of NaCl or glucose to specimens did not significantly change protein precipitation. In terms of sedimentation effectiveness with hypertriglyceridemic specimens, the methods were ranked in the following order: polyethylene glycol (pH 10, 100 g/L) greater than dextran sulfate-Mg2+ greater than heparin-Mn2+ (92 mmol/L) = polyethylene glycol (75 g/L) greater than phosphotungstate-Mg2+ greater than heparin-Mn2+ (46 mmol/L).
Warnick
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Nguyen
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Albers
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D000293:Adolescent; D000328:Adult; D000368:Aged; D001798:Blood Proteins / Q000032:analysis; D011232:Chemical Precipitation / Q000379:methods; D002648:Child; D002784:Cholesterol / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood*; D016264:Dextran Sulfate; D003911:Dextrans; D005260:Female; D006493:Heparin; D006801:Humans; D008274:Magnesium; D008297:Male; D008345:Manganese; D008875:Middle Aged; D010772:Phosphotungstic Acid; D011092:Polyethylene Glycols; D014280:Triglycerides / Q000097:blood; D014462:Ultrafiltration
D001798:Blood Proteins; D008076:Cholesterol, HDL; D003911:Dextrans; D014280:Triglycerides; D010772:Phosphotungstic Acid; D011092:Polyethylene Glycols; D008345:Manganese; D006493:Heparin; D016264:Dextran Sulfate; D002784:Cholesterol; D008274:Magnesium