title: Comparison of RNA from healthy and scrapie-infected hamster brain.
abstract: Density gradient fractions prepared from healthy or scrapie-infected hamster brain tissue enriched in plasma membrane vesicles were treated with nucleases prior to phenol extraction and ethanol precipitation. The recovered nucleic acids were 3' end-labelled and run on one-dimensional polyacrylamide gels. Autoradiography revealed the presence of low molecular weight RNAs (4S) in both healthy and scrapie samples. Two-dimensional fingerprint analysis indicated that the RNAs isolated from scrapie-infected hamsters contained oligonucleotides that were not present in RNAs isolated from healthy hamsters.
authors.forename: T L
authors.initials: TL
authors.lastname: German
authors.forename: B C
authors.initials: BC
authors.lastname: McMillan
authors.forename: B E
authors.initials: BE
authors.lastname: Castle
authors.forename: C
authors.initials: C
authors.lastname: Dees
authors.forename: W F
authors.initials: WF
authors.lastname: Wade
authors.forename: R F
authors.initials: RF
authors.lastname: Marsh
mesh_terms: D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry; D006224:Cricetinae; D012313:RNA / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis*; D012608:Scrapie / Q000378:metabolism*; D012756:Sheep; D014779:Virus Replication
chemical_list: D012367:RNA, Viral; D012313:RNA