A morphometric and quantitative microradiographic study of dental tissues in the hypopituitary dwarf mouse.
In transverse sections the areas of enamel, dentine and pulp were determined using image analysis morphometry, and the mineralization levels of enamel, dentine and alveolar bone using quantitative microradiography and a microphotometric-microdensitometric technique. There were significant differences in the ratios of dentine to pulp area, and in the enamel to total area in the incisors from dwarf and normal animals. The mean dentine to pulp ratio for dwarf incisors was more than six times greater than in normal incisors. There was also a significantly greater variation in the dentine to pulp ratio in dwarfs, which was attributed to their greater variation in pulp size. Values for mineral density of enamel, dentine and bone were similar to those found by other investigators. For example, for normal specimens, the mean mineral density of outer enamel was 2.84 g/cm3 (SD = 0.38), percentage mineralization 92%; outer labial dentine was 1.64 g/cm3 (SD = 0.15), percentage mineralization 52%; and alveolar bone was 1.53 g/cm3 (SD = 0.13), percentage mineralization 49%. The variation in mineralization within each tissue confirmed previous work in rodents. There was a consistent trend for mineralization levels of all hard tissues in the dwarf sample to be less than in the normal sample but the difference was not statistically significant for any one tissue.
Postlethwaite
K M
KM
Orthodontic Department, Queen Alexandra Hospital, Cosham, Portsmouth, England.
Roberts
G J
GJ
D000539:Alveolar Process / Q000473:pathology; D000704:Analysis of Variance; D000818:Animals; D003743:Dental Enamel / Q000473:pathology; D003786:Dental Pulp Cavity / Q000473:pathology; D003804:Dentin / Q000473:pathology; D004195:Disease Models, Animal; D004393:Dwarfism, Pituitary / Q000473:pathology*; D007180:Incisor; D051379:Mice; D008815:Mice, Inbred Strains; D008852:Microradiography; D009811:Odontometry / Q000379:methods*; D010784:Photomicrography; D014070:Tooth / Q000473:pathology*