title: Serial determinations of regional cerebral blood flow in the rat using simple chronically implanted platinum wire microelectrodes.
abstract: The ability to reliably obtain serial determinations of regional cerebral blood flow (rCBF) over time in small animals using simple and inexpensively constructed platinum-wire microelectrodes has been investigated. Repeated measurements of local cortical flow were obtained on a daily basis using the hydrogen clearance technique in a group of 18 animals, in each of which, 6 electrodes were chronically implanted. Our studies have shown the simple microelectrodes utilised to give a low variability of results, as well as being well tolerated and provoking minimal tissue reaction even over prolonged periods of time. Under identical steady-state conditions, serial determinations of rCBF were obtained for up to one week, with a maximum variation in mean values of only 12%, a result that compares favourably with the known serial determination error for the technique in the shorter term.
authors.affiliation: Cerebral Oedema Research Group, Institute of Neurology, London, U.K.
authors.forename: A
authors.initials: A
authors.lastname: Jackowski
authors.forename: A
authors.initials: A
authors.lastname: Crockard
authors.forename: R
authors.initials: R
authors.lastname: Ross-Russell
authors.forename: G
authors.initials: G
authors.lastname: Burnstock
mesh_terms: D000818:Animals; D001921:Brain / Q000098:blood supply*; D002560:Cerebrovascular Circulation; D008297:Male; D008839:Microelectrodes; D008991:Monitoring, Physiologic / Q000295:instrumentation* / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains