The isolation of intrahepatic biliary epithelial cells from normal rat livers.
A method is described for the isolation of biliary epithelial cells from rat livers by sequential treatment with EGTA, collagenase-hyaluronidase, trypsin, and deoxyribonuclease I and final separation on a discontinuous Percoll gradient using prekeratin antibodies as an immunohistochemical marker for these cells.
Gall
J A
JA
Bhathal
P S
PS
D000818:Animals; D001653:Bile Ducts, Intrahepatic / Q000166:cytology*; D002469:Cell Separation / Q000379:methods; D004847:Epithelial Cells; D007633:Keratins / Q000378:metabolism; D008099:Liver / Q000166:cytology*; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains
D007633:Keratins