Interleukin-1-inhibitory IgG in sera from some patients with rheumatoid arthritis.
Inhibition of interleukin-1 alpha (IL-1 alpha) activity was detected in 7 of 41 serum samples from patients with rheumatoid arthritis (RA). These 7 sera inhibited not only IL-1 alpha-induced endothelial cell adherence to neutrophils, but also IL-1 beta-induced endothelial cell adherence, although to a lesser extent. These sera showed no influence on tumor necrosis factor-induced endothelial cell adherence. No inhibitory activity was found in 40 sera from normal control subjects. Studies to further examine these effects included gel filtration analysis of 2 of the RA sera. The inhibitory activity was eluted near Mr 158 kd and above Mr 250 kd. Analysis by protein A affinity chromatography showed that IL-1-inhibitory activity was present in protein A-binding fractions. Purified IgG (by DE-52 column chromatography) from RA patients was found to be as potent an inhibitor as the protein A-binding fractions, which suggests that the major inhibitory activity in RA sera is attributable to IgG molecules. These purified IgG molecules also inhibited IL-1-induced proliferation of mouse thymocytes but did not influence IL-2-dependent proliferation of the CTLL-2 murine T cell line. The 7 patients whose sera showed IL-1-inhibitory activity had mild RA and low titers of rheumatoid factor. The findings, taken together, suggest a possible regulatory role of IL-1-inhibitory IgG in RA disease activity.
Suzuki
H
H
Department of Rheumatology, University of Tsukuba, Ibaraki-ken, Japan.
Akama
T
T
Okane
M
M
Kono
I
I
Matsui
Y
Y
Yamane
K
K
Kashiwagi
H
H
D000328:Adult; D000818:Animals; D001172:Arthritis, Rheumatoid / Q000097:blood*; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000302:isolation & purification / Q000502:physiology*; D007375:Interleukin-1 / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D008297:Male; D051379:Mice; D008875:Middle Aged; D009504:Neutrophils / Q000502:physiology; D012016:Reference Values; D012217:Rheumatoid Factor / Q000032:analysis; D013950:Thymus Gland / Q000166:cytology; D000515:alpha 1-Antitrypsin / Q000032:analysis
D007074:Immunoglobulin G; D007375:Interleukin-1; D000515:alpha 1-Antitrypsin; D012217:Rheumatoid Factor