title: Suppression of murine IgE responses with amino acid polymer/allergen conjugates. I. Preparation of poly-(N-methylglycine)/grass pollen extract conjugates using 4-(methylmercapto)phenyl-succinimidyl succinate as coupling reagent.
abstract: A procedure utilising the latent activating potential of the 4-(methylmercapto)phenyl ester group has been developed for the controlled, reproducible preparation of macromolecular conjugates. This ester, as part of a succinyl-bridging group, was used to couple the water-soluble, amino acid polymer, poly-(N-methylglycine) (polysarcosine), via its N-terminal secondary amine, with the nucleophilic components of the aqueous extract of a mixture of grass pollens. The products exhibit a reproducible, antigen-specific suppressive effect on the in vivo synthesis of IgE in mice.
authors.forename: N
authors.initials: N
authors.lastname: Whittall
authors.forename: D M
authors.initials: DM
authors.lastname: Moran
authors.forename: A W
authors.initials: AW
authors.lastname: Wheeler
authors.forename: G P
authors.initials: GP
authors.lastname: Cottam
mesh_terms: D000485:Allergens / Q000276:immunology*; D000818:Animals; D007073:Immunoglobulin E / Q000276:immunology*; D007165:Immunosuppression Therapy; D008024:Ligands; D046911:Macromolecular Substances; D051379:Mice; D010455:Peptides / Q000276:immunology*; D012521:Sarcosine / Q000031:analogs & derivatives* / Q000276:immunology; D013388:Succinimides
chemical_list: D000485:Allergens; D008024:Ligands; D046911:Macromolecular Substances; D010455:Peptides; D013388:Succinimides; C015475:polysarcosine; D007073:Immunoglobulin E; C044918:4-(methylmercapto)phenyl succinimidyl succinate; D012521:Sarcosine