title: Secondary structure and membrane topology of cytochrome P450s.
abstract: The secondary structure prediction of 19 microsomal cytochrome P450s from two different families was made on the basis of their amino acid sequences. It was shown that there is structural similarity between the heme-binding sites in these enzymes and those in the bacterial P450cam. An average predicted secondary structure of cytochrome P450 proteins with 70% accuracy contains about 46% alpha-helices, 12% beta-sheets, 9% beta-turns, and 33% random coils. In the region of residues 35-120 in microsomal P450s two adjacent beta alpha beta-units (the Rossmann domain), were recognized and may be available to interact with the NADPH-cytochrome P450 reductase. Using the procedure for identification of hydrophobic and membrane-associated alpha-helical segments, only one N-terminal transmembrane anchor was predicted. Also the heme-binding site may include the surface-bound helix. A model for vertebrate microsomal P450s having an amphipathic membrane protein located on the cytoplasmic side of the endoplasmic reticulum membrane, with their active center lying outside or on the bilayer border, is proposed.
authors.affiliation: Institute of Physical-Chemical Medicine, Moscow, USSR.
authors.forename: V E
authors.initials: VE
authors.lastname: Tretiakov
authors.forename: K N
authors.initials: KN
authors.lastname: Degtyarenko
authors.lastname: Uvarov VYu
authors.forename: A I
authors.initials: AI
authors.lastname: Archakov
mesh_terms: D000465:Algorithms; D000595:Amino Acid Sequence; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D008861:Microsomes / Q000201:enzymology; D008961:Models, Structural; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011549:Pseudomonas / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid
chemical_list: D003577:Cytochrome P-450 Enzyme System