Superoxide dismutase and alpha-tocopherol suppress the paraquat-induced elevation of N1-acetylspermidine and putrescine in primary culture of adult rat hepatocytes.
A transient increase in N1-acetylpolyamines and putrescine (PUT) was observed in hepatocytes at the early stage of primary culture of rat hepatocytes. After pre-culture for 36 hr when the polyamine content returned to constant levels, we tested the effects of superoxide dismutase (SOD) and alpha-tocopherol on the paraquat-induced increase in N1-acetylspermidine (N1-acetyl-SPD) and PUT in the culture. Paraquat increased (N1-acetyl-SPD in a dose-dependent manner. It also increased PUT at doses between 0.1 and 1.3 mM. Both SOD and alpha-tocopherol suppressed the increase in N1-acetyl-SPD and PUT induced by paraquat. These results suggested that superoxide anion is one of the factors which increase N1-acetyl-SPD and PUT in hepatocytes. Lipopolysaccharide (LPS) little affected the polyamine concentration in the cultured hepatocytes, though it increases polyamine in mouse liver when given in vivo. These findings suggested that the formation of superoxide anion after administration of LPS in vivo is mediated by Kupffer cells.
Sugimoto
H
H
Department of Physiology, Gunma University, Maebashi, Japan.
Matsuzaki
S
S
Hamana
K
K
Nagamine
T
T
Yamada
S
S
Suzuki
M
M
Kobayashi
S
S
D000818:Animals; D002478:Cells, Cultured; D008297:Male; D010269:Paraquat / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D011700:Putrescine / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013095:Spermidine / Q000031:analogs & derivatives* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000494:pharmacology*; D014810:Vitamin E / Q000494:pharmacology*
D014810:Vitamin E; C017988:N(1)-acetylspermidine; D013482:Superoxide Dismutase; D010269:Paraquat; D013095:Spermidine; D011700:Putrescine