Comparison of insect hemoglobins (Erythrocruorins) from Chironomus thummi thummi and Chironomus thummi piger (Diptera). The primary structure of the monomeric hemoglobin CTP III.
The monomeric hemoglobin fractions of Chironomus thummi thummi (CTT) and Chironomus thummi piger (CTP) differ in the ratio of their components. The determination of the primary structure of the component CTP III was achieved by automatic Edman degradation of the native chain, the tryptic peptides and the C-terminal fragment, obtained by cleavage at the single tryptophan residue. It revealed two chains in the ratio 1:1 which share the ambiguity threonine/isoleucine in position 57 with CTT III. Whereas one chain is identical to the CTT III hemoglobin, the other differs in having isoleucine in position 105 and alanine in position 134. The CTP monomeric hemoglobin fraction comprises 8% of a component (CTP IV A) with a more negative charge than CTT IV but with an identical sequence up to position 44. This study reveals a very high polymorphism within Chironomus species and points out the need for more data at the gene level in order to provide better understanding of this striking phenomenon.
Kleinschmidt
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Max-Planck-Institut für Biochemie, Abteilung Proteinchemie, Martinsried bei München.
Keyl
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Braunitzer
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D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002683:Chironomidae / Q000378:metabolism*; D002848:Chromatography, DEAE-Cellulose; D004175:Diptera / Q000378:metabolism*; D006454:Hemoglobins / Q000032:analysis*; D006458:Hemolymph / Q000032:analysis; D007463:Iodobenzoates; D007525:Isoelectric Focusing; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis / Q000302:isolation & purification; D014357:Trypsin
D000596:Amino Acids; D006454:Hemoglobins; D007463:Iodobenzoates; D010455:Peptides; C008154:2-iodosobenzoic acid; D014357:Trypsin