Immunoreactive anionic and cationic trypsin in human serum.
A simple method for the purification of anionic and cationic trypsinogen and trypsin from human pancreatic juice applying affinity chromatography on aprotinin coupled Sepharose is described together with the N-terminal amino acid sequences for both trypsinogens. In addition, enzyme-linked immunoabsorbent assay (ELISA) methods for the determination of anionic and cationic trypsin-like immunoreactivity (irAT and irCT) are described. Normal serum levels are 21.3 +/- 7.4 micrograms/l and 27.8 +/- 9.0 microgram/l for irAT and irCT respectively and the accuracy of these assays is 6-10%. In our population, the normal ratio between irCT and irAT in serum is 1.36 +/- 0.42. In normal serum trypsin-like immunoreactivity consists solely of trypsinogen. In acute pancreatitis there is an increase over normal of both irAT and irCT with a proportionally greater increase in irAT than irCT. Similar changes are also found in uremic patients.
Kimland
M
M
Yale University School of Medicine, New Haven CT.
Russick
C
C
Marks
W H
WH
Borgström
A
A
D000208:Acute Disease; D000595:Amino Acid Sequence; D000838:Anions; D002412:Cations; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007674:Kidney Diseases / Q000201:enzymology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010189:Pancreatic Juice / Q000201:enzymology; D010190:Pancreatic Neoplasms / Q000201:enzymology; D010195:Pancreatitis / Q000201:enzymology; D014357:Trypsin / Q000097:blood* / Q000302:isolation & purification; D014361:Trypsin Inhibitors / Q000378:metabolism; D014362:Trypsinogen / Q000097:blood* / Q000302:isolation & purification
D000838:Anions; D002412:Cations; D014361:Trypsin Inhibitors; D014362:Trypsinogen; D014357:Trypsin