Characterization of a post-column reaction-laser-induced fluorescence detector for capillary zone electrophoresis.
Several modifications have been made to a post-column labeling system for use with capillary zone electrophoresis. Fluorescence excitation is now performed with a helium-cadmium laser rather than an arc lamp. The focusability of the laser beam allows the use of larger diameter capillaries in the post-column reactor without the excessive band broadening observed previously. These larger capillaries can be assembled in the reactor much more easily. Another improvement is that the flow-rate of the labeling reagent can now be accurately controlled and determined. Incorporating these changes, the performances of two reactors with capillaries of the same dimensions are compared.
Nickerson
B
B
Department of Chemistry, University of North Carolina, Chapel Hill 27599-3290.
Jorgenson
J W
JW
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D004586:Electrophoresis / Q000379:methods*; D006736:Horses; D007834:Lasers; D009206:Myocardium / Q000378:metabolism; D009211:Myoglobin / Q000032:analysis; D013050:Spectrometry, Fluorescence
D009211:Myoglobin