[One- and two-electron reduction of ubiquinone homologs by NADH- dehydrogenase preparations from the mitochondrial respiratory chain].
The mechanism of ubiquinone homologs reduction by different preparations of mitochondrial NADH dehydrogenase: complex I within submitochondrial particles, isolated NADH-ubiquinone oxidoreductase and soluble low molecular weight NADH dehydrogenase, has been investigated. It has been shown that NADH oxidation via the rotenone-insensitive reaction is associated with one-electron reduction of low molecular weight ubiquinone homologs (Q0, Q1, Q2) to semiquinone with subsequent fast oxidation of the latter by atmospheric oxygen to form a superoxide radical. The two-electron ubiquinone reduction to quinol in the rotenone-sensitive reaction is unaccompanied by the semiquinone release from the enzyme active center into the surrounding solution.
Sled'
V D
VD
Zinich
V N
VN
Kotliar
A B
AB
D000818:Animals; D002417:Cattle; D003579:Cytochrome Reductases / Q000378:metabolism*; D004583:Electrons; D009206:Myocardium / Q000201:enzymology; D009245:NADH Dehydrogenase / Q000378:metabolism*; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D012402:Rotenone / Q000494:pharmacology; D013367:Submitochondrial Particles / Q000201:enzymology; D014451:Ubiquinone / Q000378:metabolism*
D012402:Rotenone; D014451:Ubiquinone; D003579:Cytochrome Reductases; D009245:NADH Dehydrogenase
Odno- i dvukhélektronnoe vosstanovlenie gomologov ubikhinona preparatami NADH-degidrogenazy dykhatel'noĭ tsepi mitokhondriĭ.