title: Effects of theophylline on chromosomal breakage and sister-chromatid exchange.
abstract: Frequencies of both sister-chromatid exchange (SCE) and chromosomal breakage (CB) were studied in the lymphocytes of normal individuals (10 and 7 individuals respectively). The cells were exposed in vitro to 3 different concentrations of theophylline (1, 10 and 100 micrograms/ml). A significant concentration effect of the drug was demonstrated for both SCEs and CB. Utilizing a Dunnett's test for individual comparisons, the 10 and 100 micrograms/ml concentrations both demonstrated a significant elevation of SCEs and CB compared to the untreated control cultures. This study suggests that in vitro concentrations of theophylline equal to or greater than 10 micrograms/ml, corresponding to serum levels attained during therapy, increase the frequency of SCEs and chromosome breakage in human lymphocytes.
authors.affiliation: Department of Obstetrics/Gynecology, University of Maryland, School of Medicine, Baltimore 21201.
authors.forename: P
authors.initials: P
authors.lastname: Day
authors.forename: Z
authors.initials: Z
authors.lastname: Shalaby
authors.forename: M M
authors.initials: MM
authors.lastname: Cohen
authors.forename: S S
authors.initials: SS
authors.lastname: Wasserman
authors.forename: S
authors.initials: S
authors.lastname: Schwartz
mesh_terms: D002875:Chromosomes / Q000187:drug effects*; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D009153:Mutagens; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D013806:Theophylline / Q000009:adverse effects*
chemical_list: D009153:Mutagens; D013806:Theophylline