title: Lipoprotein-mediated efflux of radiolabeled cholesterol from cells does not indicate net removal of cellular cholesterol mass.
abstract: The efflux of cholesterol from human skin fibroblasts was determined using radioisotope techniques and mass measurements. When the cells were labeled with [14C]- or [3H]-cholesterol and then incubated with very low density, low density, or high density lipoproteins or with serum, 20 to 30% of the label was released into the medium in 20 h. However, when the cellular cholesterol content was determined after incubation with various lipoproteins under identical conditions, only the heavier subfraction of high density lipoproteins (HDL3) caused a significant decrease in cellular cholesterol. This net removal of cholesterol can be observed in the cells without overloading them with cholesterol, by incubation with low density lipoproteins. Time studies indicated that at least 24 h of incubation is required to detect significant removal of cellular cholesterol. These experiments show that methods using the release of labeled cholesterol from cultured cells to determine net cholesterol removal mediated by high density lipoprotein, although currently used by many investigators, can lead to erroneous conclusions when employed without the measurement of cholesterol mass.
authors.affiliation: Atherosclerosis Research Unit, Mayo Clinic, Rochester, MN 55905.
authors.forename: S
authors.initials: S
authors.lastname: Ranganathan
authors.forename: J D
authors.initials: JD
authors.lastname: Gasman
authors.forename: H
authors.initials: H
authors.lastname: Matsuura
authors.forename: B A
authors.initials: BA
authors.lastname: Kottke
mesh_terms: D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D002784:Cholesterol / Q000378:metabolism*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007457:Iodine Radioisotopes; D008074:Lipoproteins / Q000378:metabolism*; D008075:Lipoproteins, HDL / Q000302:isolation & purification / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000302:isolation & purification / Q000378:metabolism; D014316:Tritium
chemical_list: D002250:Carbon Radioisotopes; D007457:Iodine Radioisotopes; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D014316:Tritium; D002784:Cholesterol