In situ detection of DNA-binding proteins in herpes simplex virus type 1-infected cells.
An in situ assay for detecting DNA-binding proteins in herpes simplex virus type 1 (HSV-1)-infected cells is described. Seventeen HSV-induced DNA-binding species were visible with nicked, double-stranded DNA as a substrate, while fourteen virus-induced DNA-binding fractions were present in gels containing nuclease-treated, single-stranded DNA. The effects of HSV on cellular DNA-binding protein expression could also be seen. The resolution of DNA-binding fractions was dependent upon the type of DNA substrate utilized, high salt extraction of DNA-binding components and their physical separation from infected cell DNAs, dialysis of the high salt and the length of DNase treatment of gels following electrophoresis.
Mohammad
F
F
Department of Microbiology, University of New Hampshire, Durham 03824.
Farber
E
E
D000818:Animals; D002498:Centrifugation; D004268:DNA-Binding Proteins / Q000032:analysis*; D003851:Deoxyribonucleases; D004591:Electrophoresis, Polyacrylamide Gel; D004996:Ethidium; D012260:Ribonucleases; D018139:Simplexvirus / Q000032:analysis*; D014709:Vero Cells; D014764:Viral Proteins / Q000032:analysis*
D004268:DNA-Binding Proteins; D014764:Viral Proteins; D003851:Deoxyribonucleases; D012260:Ribonucleases; D004996:Ethidium