title: Changes in the intracellular calcium ion concentration in the gastric mucosa in a rat ischemia-reperfusion model.
abstract: To investigate the role of the intracellular calcium ion in the development of acute gastric mucosal lesions, phosphorylase a activity was measured as an index of the intracellular calcium ion concentration ([Ca2+]i), using Lowry's method, in the rat ischemia-reperfusion model. [Ca2+]i increased significantly at the end of the ischemic state without acute mucosal lesions (AGML). After reinfusion, [Ca2+]i showed a slight increase and AGML developed. Continual intravenous infusion of the calcium channel blocker, diltiazem (1 mg/kg/hr), inhibited the increase in [Ca2+]i in the ischemic state and reduced the development of AGML after reinfusion. These results suggest that the increase in [Ca2+]i in the ischemic state plays an important role in the development of AGML.
authors.affiliation: Department of Medicine, Osaka University Medical School, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Hayashi
authors.forename: M
authors.initials: M
authors.lastname: Tsujii
authors.forename: T
authors.initials: T
authors.lastname: Itoh
authors.forename: H
authors.initials: H
authors.lastname: Sakura
authors.forename: S
authors.initials: S
authors.lastname: Tsuji
authors.forename: H
authors.initials: H
authors.lastname: Tanimura
authors.forename: T
authors.initials: T
authors.lastname: Ogihara
authors.forename: H
authors.initials: H
authors.lastname: Yoshihara
authors.forename: S
authors.initials: S
authors.lastname: Kawano
authors.forename: N
authors.initials: N
authors.lastname: Sato
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004110:Diltiazem / Q000494:pharmacology; D005753:Gastric Mucosa / Q000098:blood supply* / Q000378:metabolism / Q000648:ultrastructure; D007425:Intracellular Membranes / Q000378:metabolism*; D007477:Ions; D008297:Male; D009994:Osmolar Concentration; D010762:Phosphorylase a / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D015427:Reperfusion Injury / Q000378:metabolism*
chemical_list: D007477:Ions; D010762:Phosphorylase a; D004110:Diltiazem; D002118:Calcium