Antigenic reactivity of a monoclonal antibody against Amoeba proteus actin.
The reactivity of a monoclonal antibody against actin of Amoeba proteus with actins from other sources was examined. The monoclonal antibody cross-reacted with actins from vertebrate muscles, human erythrocytes, and Acanthamoeba castellanii, but it did not react with Naegleria gruberi actin. The amoeba actin was resolved into 3 bands with isoelectric points of 5.96, 6.03 and 6.10 in electrofocusing gels and they corresponded to 3 peptide spots reacting with the antibody on 2-dimensional immunoblots.
Ahn
T I
TI
Department of Zoology, University of Tennessee, Knoxville 37996.
Jeon
K W
KW
D000199:Actins / Q000276:immunology*; D000656:Amoeba / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000913:Antibodies, Protozoan / Q000276:immunology; D000953:Antigens, Protozoan / Q000276:immunology*; D003429:Cross Reactions; D015151:Immunoblotting; D007525:Isoelectric Focusing
D000199:Actins; D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan