Development and characterization of monoclonal antibodies with specificity for metallic radioisotope chelators linked to antibodies and other proteins.
Monoclonal antibodies with specificity for the diethylenetriaminepentaacetic acid (DTPA) portion of the GYK-DTPA (glycyl-tyrosyl-lysine-DTPA) linker structure used to chelate metals to immunoglobulins have been prepared. A significant proportion of these antibodies were lambda light chain isotype. Competition assays demonstrated that DTPA, rather than GYK, was the binding site of the antibodies tested. These monoclonal antibodies should be useful reagents for use in assays specific for the presence of the common linker structure used to chelate metallic radioisotopes to monoclonal antibodies. The antibodies tested did not discriminate between chelated and unchelated DTPA.
Gillette
R W
RW
Molecular and Cellular Biology, Cytogen Corporation, Princeton, NJ 08540.
Singleton
J
J
Janowicz
A
A
Gilman
S C
SC
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000918:Antibody Specificity; D001667:Binding, Competitive; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas; D007074:Immunoglobulin G / Q000276:immunology; D007204:Indium / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000378:metabolism; D004369:Pentetic Acid / Q000378:metabolism / Q000031:analogs & derivatives; D011868:Radioisotopes
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D009842:Oligopeptides; D011868:Radioisotopes; D007204:Indium; C062250:glycyl-tyrosyl-lysine-DTPA; D004369:Pentetic Acid