Injection of anti-thy-1.2 serum breaks genetic resistance of mice against herpes simplex virus.
Nude mice have been shown to be as resistant to intraperitoneal infection with herpes simplex virus type 1 (HSV) as their heterozygous littermates. Here we document that both activation of natural killer (NK) cells and interferon induction were normal in nu/nu mice after injection of HSV. Injection of silica caused increased mortality by HSV in C57BL/6 mice. Silica, in addition, led to a significant reduction of NK cell activity but had no effect on the interferon response. Treatment of C57BL/6 mice with anti-asialo GM1 (an antiserum with a predominant effect on NK cells) caused complete abolition of the NK cell response, but had no effect on interferon induction or virus-induced mortality. In further studies a monoclonal anti-thy-1.2 antibody was utilized which possessed high activity in vivo in depleting T cell responses in mice. Injection of anti-thy-1.2 decreased NK cell activation but was without effect on the interferon response. Unexpectedly, in view of the data in nu/nu mice, this antibody increased HSV-induced mortality in C57BL/6 mice. Similar data were obtained when anti-thy-1.2 was injected into nu/nu mice. Our results are compatible with the hypothesis that T cell precursors sensitive to anti-thy-1.2 present in homozygous nude mice play a role in resistance against HSV. Furthermore, the data in the euthymic mice may indicate a role of T cells in the primary resistance of mice against HSV.
Chmielarczyk
W
W
Engler
H
H
Ernst
R
R
Opitz
U
U
Kirchner
H
H
D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology; D005677:G(M1) Ganglioside; D006028:Glycosphingolipids / Q000276:immunology; D006561:Herpes Simplex / Q000276:immunology*; D007106:Immune Sera; D007113:Immunity, Innate; D007372:Interferons / Q000096:biosynthesis; D007518:Isoantibodies; D007694:Killer Cells, Natural / Q000276:immunology*; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D010835:Phytohemagglutinins / Q000494:pharmacology; D012822:Silicon Dioxide / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology*
D006028:Glycosphingolipids; D007106:Immune Sera; D007518:Isoantibodies; D010835:Phytohemagglutinins; C035678:anti-Thy antibody; D003208:Concanavalin A; D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside; D012822:Silicon Dioxide; D007372:Interferons