Aromatase activity in individual day-11 pig blastocysts.
Blastocysts were flushed from both uterine horns of 10 gilts on Day 11 of pregnancy. In these gilts 15.1 +/- 2.3 (mean +/- s.d.) corpora lutea were present and 12.7 +/- 3.1 spherical blastocysts were recovered. In all the gilts variation in blastocyst diameter was observed. Aromatase activity was measurable in 118 of 121 examined blastocysts and varied from 0.005 to 19.330 pmol [1 beta-3H]androstenedione converted into 3H2O in 20 min (mean 1.31). This variation in aromatase activity reflected a difference between and within gilts. Of the total variation between all blastocysts, 67% was due to differences between gilts. A positive exponential relationship existed between blastocyst diameter and aromatase activity, but this relationship was different between gilts (P less than 0.0001). The observed variation in aromatase activity may be caused by differences in germ layer differentiation of the blastocysts.
van der Meulen
J
J
Department of Animal Physiology, Agricultural University, Wageningen, The Netherlands.
te Kronnie
G
G
van Deursen
R
R
Geelen
J
J
D000818:Animals; D001141:Aromatase / Q000378:metabolism*; D001755:Blastocyst / Q000166:cytology / Q000201:enzymology*; D005260:Female; D013552:Swine
D001141:Aromatase