Co-localization of adrenergic receptors and vitamin-D-dependent calcium-binding protein (calbindin) in the dopaminergic amacrine cells of the rat retina.
Using antisera against tyrosine hydroxylase (TH) and purified beta 2-adrenergic receptors (beta 2-AdR), we found that TH- and AdR-like immunoreactivities coexisted in large amacrine cells. These findings indicated an association between dopamine-containing amacrine cells and adrenergic amacrine cells. The present study also showed that amacrine cells with TH-like immunoreactivity have vitamin-D-dependent calcium-binding protein (calbindin, 27,000 kDa)-like immunoreactivity as well, suggesting that calbindin plays an important postsynaptic role in dopaminergic amacrine cells.
Ishimoto
I
I
Department of Ophthalmology, Osaka University Medical School, Japan.
Kiyama
H
H
Hamano
K
K
Shiosaka
S
S
Malbon
C C
CC
Nakauchi
M
M
Emson
P C
PC
Manabe
R
R
Tohyama
M
M
D000818:Animals; D064026:Calbindins; D004298:Dopamine / Q000502:physiology*; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D012160:Retina / Q000166:cytology / Q000378:metabolism*; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D064026:Calbindins; D011943:Receptors, Adrenergic, beta; D064030:S100 Calcium Binding Protein G; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine