Coupling capillary zone electrophoresis and continuous-flow fast atom bombardment mass spectrometry for the analysis of peptide mixtures.
Combined capillary zone electrophoresis (CZE)-continuous-flow fast atom bombardment (CF-FAB) mass spectrometry is described for the analysis of mixtures of peptides. A 90 cm x 50 microns I.D. fused-silica capillary column was used for electrophoretic separations and was connected to the CF-FAB probe via an interface which allows a total flow into the mass spectrometer of about 5 microliters/min. Solutions of peptides were pneumatically loaded onto the CZE capillary, providing sample amounts of 0.1-20 pmol. The magnetic mass spectrometer was scanned over the desired mass range, usually between m/z 500 and 2500. Results are shown for separation and analysis of mixtures of synthetic peptides and also for protease digests of recombinant human growth hormone and horse heart cytochrome c.
Caprioli
R M
RM
Analytical Chemistry Center, University of Texas Medical School, Houston 77030.
Moore
W T
WT
Martin
M
M
DaGue
B B
BB
Wilson
K
K
Moring
S
S
D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods*; D013058:Mass Spectrometry; D010455:Peptides / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D010455:Peptides