Oxidative burst capability of human monocyte subsets defined by high and low HLA-DR expression.
There is a wide range in the expression of major histocompatability complex (MHC) class II HLA-DR molecules on freshly isolated human peripheral blood monocytes from one individual. This study determined whether the variability in HLA-DR expression on monocytes correlated with their oxidative burst capability after treatment with either phorbol myristate acetate (PMA) or calcium ionophore A23187. Freshly obtained cells were incubated with 2',7'-dichlorofluorescin diacetate (DCFH-DA), a dye which is utilized for the determination of cell oxidative potential by flow cytometry. Cells were stimulated, and then stained with phycoerythrin (PE)-labeled monoclonal antibodies (mAb). Two-color flow cytometry was used to determine changes in DCFH-DA (green) fluorescence which corresponded to oxidative product formation by cells which stained only with the PE-labeled mAb (monocytes). While monocytes with a high or low surface density of HLA-DR differed substantially in HLA-DR expression, only small differences in oxidative capability between "high" and "low" subsets were observed. Thus, monocytes which are very different in HLA-DR expression possess very similar oxidative burst capabilities.
Spear
G T
GT
Department of Immunology/Microbiology, Rush Presbyterian St. Luke's Medical Center, Chicago, Illinois 60612.
June
R A
RA
Landay
A L
AL
D000328:Adult; D015703:Antigens, CD / Q000032:analysis / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis; D001770:Blood Bactericidal Activity; D006684:HLA-DR Antigens / Q000032:analysis* / Q000276:immunology; D006801:Humans; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology*
D015703:Antigens, CD; D000954:Antigens, Surface; D006684:HLA-DR Antigens