Human plasma kininogens are identical with alpha-cysteine proteinase inhibitors. Evidence from immunological, enzymological and sequence data.
Human high- and low-Mr kininogens were shown to be potent inhibitors of cysteine proteinases such as cathepsin L and papain (Ki = 17-48 pM). A strong immunological cross-reaction between the kininogens and low-Mr alpha-cysteine proteinase inhibitor from human plasma was found. Comparison of partial amino acid sequences from high- and low-Mr kininogen and low-Mr alpha-cysteine proteinase inhibitor demonstrated sequence identity for all segments analyzed. These findings suggest that the kininogens and the alpha-cysteine proteinase inhibitors from human plasma are identical proteins.
Müller-Esterl
W
W
Fritz
H
H
Machleidt
W
W
Ritonja
A
A
Brzin
J
J
Kotnik
M
M
Turk
V
V
Kellermann
J
J
Lottspeich
F
F
D000595:Amino Acid Sequence; D056668:Cathepsin L; D002403:Cathepsins / Q000037:antagonists & inhibitors; D003546:Cysteine Endopeptidases; D010450:Endopeptidases; D000939:Epitopes / Q000276:immunology; D006801:Humans; D005779:Immunodiffusion; D007610:Kallikreins / Q000378:metabolism; D007704:Kininogens / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology*; D010206:Papain / Q000037:antagonists & inhibitors; D010446:Peptide Fragments; D011480:Protease Inhibitors; D014357:Trypsin / Q000378:metabolism
D000939:Epitopes; D007704:Kininogens; D010446:Peptide Fragments; D011480:Protease Inhibitors; D002403:Cathepsins; D010450:Endopeptidases; D007610:Kallikreins; D014357:Trypsin; D003546:Cysteine Endopeptidases; C534281:CTSL protein, human; D056668:Cathepsin L; D010206:Papain