Differential expression of the IL-2 receptor subunits, p55 and p75 on various populations of primary peripheral blood mononuclear cells.
Unstimulated PBL were examined for expression of IL-2R subunits, IL-2Rp55 and IL-2Rp75, by two-color flow cytometric analyses using mAb. NKH-1+ non-T non-B cells expressed IL-2Rp75 but not IL-2Rp55, and the IL-2Rp75 sites on purified NKH-1+ cells were determined to be 1630 sites/cell by binding of 125I-labeled TU27 mAb specific for IL-2Rp75. In the CD4+ T cell population, IL-2Rp55+ cells were significantly detected, but little or marginally of the IL-2Rp75+ cells. However, IL-2Rp75+ cells were significantly detected, but little of the IL-2Rp55+ cells in the CD8+ T cell population. The IL-2Rp75 sites on CD8+ T cells were estimated at approximate 180-410 sites/cell. In the CD4+ T cells, expression of IL-2Rp75 as well as IL-2Rp55 was induced by stimulation with PHA. IL-2Rp75+ cells, but not IL-2Rp55+ cells, were also detected in the CD14+ monocyte population. In the CD20+ B cell population, a small number of IL-2Rp55+ cells was detected, but little of the IL-2Rp75+ cells.
Ohashi
Y
Y
Department of Microbiology, Tohoku University School of Medicine, Sendai, Japan.
Takeshita
T
T
Nagata
K
K
Mori
S
S
Sugamura
K
K
D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D015415:Biomarkers / Q000032:analysis; D005434:Flow Cytometry; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008565:Membrane Proteins / Q000032:analysis; D051379:Mice; D010641:Phenotype; D010835:Phytohemagglutinins / Q000494:pharmacology; D015375:Receptors, Interleukin-2 / Q000032:analysis*; D013601:T-Lymphocytes / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D015415:Biomarkers; D008565:Membrane Proteins; D010835:Phytohemagglutinins; D015375:Receptors, Interleukin-2