A note on the isolation and propagation of lytic phages from Streptococcus uberis and their potential for strain typing.
Thirty-eight of 98 strains of Streptococcus uberis were shown to be carrying lysogenic phage. Although propagating strains were rare, host modification by field strains sensitive to phage was used to increase the lytic spectra. When 120 nationally-collected strains were challenged with 25 phages, selected on the basis of differing lytic spectra and propagating strains, 30% were susceptible to at least one phage, increasing to 42% when 480 strains from a single farm were considered. A typing system based on susceptibility to lytic phage was considered feasible.
Hill
A W
AW
AFRC Institute for Animal Health, Compton Laboratory, Newbury, Berkshire, UK.
Brady
C A
CA
D000818:Animals; D001434:Bacteriophage Typing; D001435:Bacteriophages / Q000254:growth & development / Q000302:isolation & purification* / Q000648:ultrastructure; D002417:Cattle; D008414:Mastitis, Bovine / Q000382:microbiology*; D008854:Microscopy, Electron; D013291:Streptococcus / Q000145:classification*