Human glioblastoma cells persistently infected with simian virus 40 carry nondefective episomal viral DNA and acquire the transformed phenotype and numerous chromosomal abnormalities.
A stable, persistent infection of A172 human glioblastoma cells with simian virus 40 (SV40) was readily established after infection at an input of 450 PFU per cell. Only 11% of the cells were initially susceptible to SV40, as shown by indirect immunofluorescent staining for the SV40 T antigen at 48 h. However, all cells produced T antigen by week 11. In contrast, viral capsid proteins were made in only about 1% of the cells in the established carrier system. Weekly viral yields ranged between 10(4) and 10(6) PFU/ml. Most of the capsid protein-producing cells contained enormous aberrant (lobulated or multiple) nuclei. Persistent viral DNA appeared in an episomal or "free" state exclusively in Southern blots and was indistinguishable from standard SV40 DNA by restriction analysis. Viral autointerference activity was not detected, and yield reduction assays did not indicate defective interfering particle activity, further implying that variant viruses were not a factor in this carrier system. Interferon was also not a factor in the system, as shown by direct challenge with vesicular stomatitis virus. Persistent infection resulted in cellular growth changes (enhanced saturation density and plating efficiency) characteristic of SV40 transformation. Persistent infection also led to an increased frequency of cytogenetic effects. These included sister chromatid exchanges, a variety of chromosomal abnormalities (ring chromosomes, acentric fragments, breaks, and gaps), and an increase in the chromosome number. Nevertheless, the persistently infected cells continued to display a bipolar glial cell-like morphology with extensive process extension and intercellular contacts.
Norkin
L C
LC
Steinberg
V I
VI
Kosz-Vnenchak
M
M
D000957:Antigens, Viral, Tumor / Q000032:analysis; D002213:Capsid / Q000096:biosynthesis; D002460:Cell Line; D002467:Cell Nucleus / Q000382:microbiology / Q000648:ultrastructure; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D002869:Chromosome Aberrations; D004279:DNA, Viral; D005910:Glioma / Q000235:genetics / Q000382:microbiology*; D006801:Humans; D007372:Interferons / Q000096:biosynthesis; D007621:Karyotyping; D010641:Phenotype; D010957:Plasmids; D013539:Simian virus 40 / Q000235:genetics / Q000276:immunology / Q000502:physiology*
D000957:Antigens, Viral, Tumor; D004279:DNA, Viral; D007372:Interferons