Effects of inducers of drug metabolism on basic hepatic forms of mouse glutathione transferase.
The cytosolic glutathione transferases (GSTs) with basic pI values have been studied in mouse liver after treatment with 2,3-t-butylhydroxyanisole (BHA), cafestol palmitate (CAF), phenobarbital (PB), 3-methylcholanthrene (3-MC) and trans-stilbene oxide (t-SBO). The cytosolic GST activity was induced by all compounds except for 3-MC. Three forms of GST were isolated by means of affinity chromatography and f.p.l.c. The examination of protein profiles and enzymic activities with specific substrates showed that the three GSTs correspond to those found in control animals, i.e. GSTs MI, MII and MIII. The class Mu GST MIII accounted for the major effect of induction, whereas the class Alpha GST MI and the class Pi GST MII were unchanged or somewhat down-regulated. The greatest induction was obtained with BHA, PB and CAF. The activities of other glutathione-dependent enzymes were also studied. An increase in glutathione reductase and thioltransferase activities was observed after BHA, PB or CAF treatment; glyoxalase I and Se-dependent glutathione peroxidase were depressed in comparison with the control group in all cases studied.
Di Simplicio
P
P
Department of Environmental Biology, University of Siena, Italy.
Jensson
H
H
Mannervik
B
B
D000818:Animals; D002083:Butylated Hydroxyanisole / Q000494:pharmacology; D003600:Cytosol / Q000201:enzymology; D004224:Diterpenes / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D054477:Glutaredoxins; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000096:biosynthesis / Q000378:metabolism*; D007527:Isoenzymes / Q000096:biosynthesis / Q000378:metabolism; D007791:Lactoylglutathione Lyase / Q000378:metabolism; D008099:Liver / Q000033:anatomy & histology / Q000201:enzymology*; D008748:Methylcholanthrene / Q000494:pharmacology; D051379:Mice; D009929:Organ Size / Q000187:drug effects; D010088:Oxidoreductases / Q000378:metabolism; D010634:Phenobarbital / Q000494:pharmacology; D011490:Protein Disulfide Reductase (Glutathione); D013267:Stilbenes / Q000494:pharmacology
D004224:Diterpenes; D054477:Glutaredoxins; D007527:Isoenzymes; D013267:Stilbenes; D002083:Butylated Hydroxyanisole; D008748:Methylcholanthrene; C033998:cafestol palmitate; D010088:Oxidoreductases; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D011490:Protein Disulfide Reductase (Glutathione); D005982:Glutathione Transferase; D007791:Lactoylglutathione Lyase; C025906:stilbene oxide; D010634:Phenobarbital