The UW solution for canine kidney preservation. Its specific effect on renal hemodynamics and microvasculature.
The preservation effects of UW solution on renal hemodynamics and microvascular systems were studied in canine kidney autografts. In 72-hr UW-preserved kidneys, the microvessels of both cortex and medulla were completely visualized with silicon rubber compound 1 hr after reperfusion. Histology also showed extremely well-preserved arterioglomerular and tubular systems. These results were correlated with good renal blood flow, prompt recovery of posttransplant graft function, and 100% two-week survival of dogs. In contrast, kidneys preserved for 72 hr with Euro-Collins solution showed necrotic and obstructive changes of the microvasculature and deterioration of renal hemodynamics. In 120-hr UW-preserved kidneys, the microcirculation of the medullary region became poor after reflow when there was fairly intact perfusion of the cortical region, indicating an ischemia-related intrarenal blood flow maldistribution. The 120-hr kidneys subsequently failed in spite of having a good blood flow and morphologically well-maintained microvasculature after reperfusion. These data demonstrated that much, but not all, of the beneficial effect of UW solution in kidney preservation might be attributed to its remarkable protection of renal microvasculature. Correction of intrarenal blood maldistribution caused by a discrepancy in tolerance to ischemia of the vascular and tubular systems might be important in successfully preserving the kidney for 120 hr.
Ueda
Y
Y
Department of Surgery, University Health Center of Pittsburgh, Pennsylvania.
Todo
S
S
Imventarza
O
O
Furukawa
H
H
Oks
A
A
Wu
Y M
YM
Oguma
S
S
Starzl
T E
TE
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D004285:Dogs; D005260:Female; D005978:Glutathione; D006439:Hemodynamics / Q000187:drug effects*; D007328:Insulin; D007668:Kidney / Q000166:cytology / Q000502:physiology; D016030:Kidney Transplantation; D008297:Male; D008833:Microcirculation / Q000187:drug effects; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D011887:Raffinose; D012079:Renal Circulation / Q000187:drug effects*; D012996:Solutions / Q000494:pharmacology*
D007328:Insulin; D019188:Organ Preservation Solutions; D012996:Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose