Comparison of Alternaria tenuis extracts prepared from different raw materials.
Alternaria extracts, prepared from three different sources under the same conditions, were compared by several biochemical/immunochemical methods. Two raw materials (A,G) contained mostly mycelia. The third raw material (C) contained mycelia (35%) and spores (65%). Extracts A and G were different in total allergenic potency, antigen/allergen pattern, and in the Alt 1 content. Extract C was of similar total allergenic potency as extract A but showed a somewhat different antigen/allergen pattern and a different Alt 1 content. Although there were some compositional differences among the extracts, all extracts regardless of source materials (spores and mycelia) demonstrated strong RAST inhibition activity and parallelism of the inhibition curves.
Wahl
R
R
Allergopharma, Joachim Ganzer KG, Reinbek, FRG.
Oliver
J D
JD
Hauck
P R
PR
Winter
H G
HG
Maasch
H J
HJ
D000485:Allergens / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification; D000528:Alternaria / Q000276:immunology*; D002241:Carbohydrates / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000032:analysis; D007123:Immunoelectrophoresis, Two-Dimensional; D007525:Isoelectric Focusing; D003904:Mitosporic Fungi / Q000276:immunology*; D011852:Radioallergosorbent Test
D000485:Allergens; D002241:Carbohydrates; D005656:Fungal Proteins