Pregnenolone, testosterone, and estradiol in the migratory locust Locusta migratoria; a gas chromatographical-mass spectrometrical study.
Homogenates of ovaria as well as testes from the locust Locusta migratoria were analyzed for the presence of pregnenolone, testosterone, and estradiol by gas chromatography followed by mass spectrometry. Selected ion monitoring analyses revealed that the molecular ion and two characteristic fragment ions of pregnenolone, the molecular ion with its isotopes and two characteristic mass fragments of testosterone, and the molecular ion, its isotopes and four characteristic fragment ions of estradiol, were present at the proper retention times and with the correct abundance ratios.
Novak
F J
FJ
Catholic University of Leuven, Zoological Institute, Belgium.
Lambert
J G
JG
D000818:Animals; D004958:Estradiol / Q000032:analysis*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006110:Grasshoppers / Q000378:metabolism*; D008297:Male; D010053:Ovary / Q000032:analysis / Q000378:metabolism; D011284:Pregnenolone / Q000032:analysis*; D011863:Radioimmunoassay; D013737:Testis / Q000032:analysis / Q000378:metabolism; D013739:Testosterone / Q000032:analysis*
D013739:Testosterone; D004958:Estradiol; D011284:Pregnenolone