[The acridine orange test. A new parameter in assessing the fertilizing capacity of spermatozoa].
Simultaneously with in vitro fertilization (IVF), the acridine orange (AO) test was performed on 51 patients according to the method of Tejada et al. This test allows a differentiation of spermatozoa with intact (double-stranded) and denatured (single-sanded) DNA. A significant relationship was demonstrated between the percentage of green-fluorescing (intact) sperm and the results of IVF. For both, the groups with (n = 40) and without (n = 11) fertilization of human eggs, a positive correlation was found between AO-test and sperm motility, and AO-test and normal sperm morphology.
Sterzik
K
K
Abteilung Frauenheilkunde und Geburtshilfe, Universität Ulm.
Rosenbusch
B
B
Sasse
V
V
Wild
E
E
Hütter
W
W
Wolf
A
A
D000165:Acridine Orange; D000328:Adult; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007248:Infertility, Male / Q000175:diagnosis / Q000503:physiopathology; D008297:Male; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D013081:Sperm Motility / Q000502:physiology*; D013084:Sperm-Ovum Interactions / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology
D000165:Acridine Orange
Der Acridin-Orange-Test. Ein neuer Parameter zur Beurteilung der Befruchtungsfähigkeit von Spermatozoen.