Serum-inducible expression of transfected human c-myc genes.
Activation of the c-myc proto-oncogene is implicated in the initiation or progression of many vertebrate cancers. In nontransformed cells, the expression of c-myc is induced by growth factors. Studies have indicated that such induction is effected by multiple mechanisms. To study regulation of c-myc expression, a transfection system has been developed in which introduced c-myc genes exhibit serum-responsive activity. The responsiveness assayed is not mediated by increased transcription initiation. Rather, it is effected at a point(s) between transcription and stabilization of the RNA.
Richman
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Department of Biology, Yale University, New Haven, Connecticut 06511-8112.
Hayday
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D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D005347:Fibroblasts; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D006133:Growth Substances / Q000097:blood / Q000494:pharmacology*; D006801:Humans; D009857:Oncogenes; D000090063:Proto-Oncogene Mas; D011519:Proto-Oncogenes; D051381:Rats; D012260:Ribonucleases / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection
D006133:Growth Substances; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D012260:Ribonucleases