title: Identification of species D, a new member of the Anopheles quadrimaculatus species complex: a biochemical key.
abstract: Sibling species D, a new member of the Anopheles quadrimaculatus species complex was identified in collections from Pickwick Lake, Tishomingo County, Mississippi and Choctawhatchee, Bay County, in West Florida. This species occurred sympatrically with the previously described species, A, B and C. Evidence for identification of species D includes diagnostic allozymes, a lack of polytene chromosomes in the ovarian nurse cells, and inviability of F1 progeny and lack of sperm transfer in hybridization crosses. An electrophoretic taxonomic key for distinguishing species D from A, B and C is presented.
authors.affiliation: U.S. Department of Agriculture, Insects Affecting Man and Animals Research Laboratory, Gainesville, FL 32604.
authors.forename: S K
authors.initials: SK
authors.lastname: Narang
authors.forename: P E
authors.initials: PE
authors.lastname: Kaiser
authors.forename: J A
authors.initials: JA
authors.lastname: Seawright
mesh_terms: D000818:Animals; D000852:Anopheles / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D002871:Chromosome Banding; D002875:Chromosomes; D003433:Crosses, Genetic; D004592:Electrophoresis, Starch Gel; D005260:Female; D005431:Florida; D006579:Heterozygote; D008297:Male; D008922:Mississippi; D011110:Polymorphism, Genetic; D013714:Tennessee