Growth inhibition and induction of phenotypic alterations by L-histidinol in B16 mouse melanoma cells.
L-Histidinol, a histidine analog was recently shown to be an inducer of differentiation in the promyelocytic cell line HL-60. In the present study we show that L-histidinol inhibits the growth of B16 melanoma cells in vitro. Growth inhibition is accompanied by phenotypic alterations that include a marked increase in the activities of NADPH cytochrome c reductase and gamma glutamyl transpeptidase, lipid accumulation and cell enlargement. These phenotypic alterations are similar to those induced by other chemical inducers of differentiation in melanoma cells.
Nordenberg
J
J
Rogoff Research Institute, Petah-Tikva, Israel.
Wasserman
L
L
Gutman
H
H
Beery
E
E
Novogrodsky
A
A
D000818:Animals; D001391:Azo Compounds; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line / Q000187:drug effects; D006641:Histidinol / Q000494:pharmacology*; D006651:Histocytochemistry; D007093:Imidazoles / Q000494:pharmacology*; D008055:Lipids / Q000032:analysis; D008545:Melanoma / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D009251:NADPH-Ferrihemoprotein Reductase / Q000032:analysis; D010641:Phenotype; D013053:Spectrophotometry; D005723:gamma-Glutamyltransferase / Q000032:analysis
D001391:Azo Compounds; D007093:Imidazoles; D008055:Lipids; D006641:Histidinol; D009251:NADPH-Ferrihemoprotein Reductase; D005723:gamma-Glutamyltransferase; C011049:oil red O