Autoantibodies to the basal cells of squamous epithelium react with thymic epithelial cells.
Sera from carriers of hepatitis B surface antigen (17 out of 21), which reacted in immunofluorescence with the basal cell layer (BCL) of squamous epithelium, were also shown to react with a thymic stellate epithelial cell (SEC) characterized by long, dendritic-like cytoplasmic processes. Absorption of the autoantibodies against BCL of squamous epithelium (BCL-Ab) with a thymic homogenate abolished the reactivity with BCL and SEC, demonstrating that the same antigenic determinant was recognized in both cells. In the human thymus, SEC were present both in the cortex and in the medulla. In the outer cortex SEC delineated the septal spaces. SEC were also stained by anti-HLA-DR (Ia) but not by antiactin monoclonal antibodies. The morphology and distribution of SEC were similar to those of the previously described thymic epithelial cells containing alpha-1 thymosin (K. Hirokawa, J. E. McClure, and A. L. Goldstein, Thymus 4, 19, 1982). BCL-Ab were also found to react with five human epithelial thymomas. BCL-Ab seemed to be useful for further characterization of the thymic epithelial cells and for the immunodiagnosis of thymoma.
Lenkei
R
R
Biberfeld
G
G
Magnius
L O
LO
Fagraeus
A
A
Biberfeld
P
P
D000199:Actins / Q000276:immunology; D000906:Antibodies / Q000494:pharmacology; D000911:Antibodies, Monoclonal; D001323:Autoantibodies / Q000502:physiology*; D004847:Epithelial Cells; D005455:Fluorescent Antibody Technique; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006801:Humans; D009928:Organ Specificity; D013194:Staining and Labeling; D013945:Thymoma / Q000276:immunology; D013950:Thymus Gland / Q000166:cytology*
D000199:Actins; D000906:Antibodies; D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II