title: DNA damage induced by some bile acids, evaluated with alkaline elution technique.
abstract: Bile acids are promoting agents in colon carcinogenesis. In this work we have tried to characterize the DNA alteration induced by bile acids in Sprague-Dawley male rats. Confirming previous findings, a clear increase in elution rate was observed at alkaline pH. No effect could be observed when the nuclei were washed before the elution, in condition totally unsuitable for the repair of the type of DNA damage induced by typical genotoxic agents. We advanced the hypothesis that the increased alkaline elution rate observed with bile acids could be independent of DNA fragmentation and related to changes in chromatin structure.
authors.forename: M
authors.initials: M
authors.lastname: Peluso
authors.forename: V
authors.initials: V
authors.lastname: Pisano
authors.forename: D
authors.initials: D
authors.lastname: De Martino
authors.forename: A M
authors.initials: AM
authors.lastname: Orengo
authors.forename: P
authors.initials: P
authors.lastname: Russo
authors.forename: M
authors.initials: M
authors.lastname: Pala
authors.forename: R
authors.initials: R
authors.lastname: Bordone
authors.forename: M
authors.initials: M
authors.lastname: Sanguineti
authors.forename: S
authors.initials: S
authors.lastname: Parodi
mesh_terms: D000818:Animals; D001647:Bile Acids and Salts / Q000009:adverse effects*; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D006863:Hydrogen-Ion Concentration; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D001647:Bile Acids and Salts; D004247:DNA