Human serum amyloid A protein. Behaviour in aqueous and urea-containing solutions and antibody production.
Human serum amyloid A protein (apo-SAA) can be prepared by gel filtration of delipidated acute-phase high-density lipoprotein in the presence of urea. The resultant apo-SAA is soluble (greater than 90% solubility) in a wide range of buffer solutions, with all of the six major isoforms of apo-SAA being equally soluble. In urea-containing solutions the isoforms behave qualitatively differently in various urea concentrations, probably reflecting subtle primary-structure variations. The higher-pI isoforms are only completely unfolded at greater than 7 M-urea. By immunizing with apo-SAA adsorbed to acid-treated bacteria (Salmonella minnesota R595), high-titre antibodies can easily be elicited in rabbits.
Strachan
A F
AF
Department of Internal Medicine, University of Stellenbosch Medical School, Tygerberg, South Africa.
Shephard
E G
EG
Bellstedt
D U
DU
Coetzee
G A
GA
van der Westhuyzen
D R
DR
de Beer
F C
FC
D000327:Adsorption; D000906:Antibodies / Q000276:immunology*; D000941:Antigens / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007114:Immunization; D007526:Isoelectric Point; D008075:Lipoproteins, HDL / Q000032:analysis; D011487:Protein Conformation / Q000187:drug effects; D012475:Salmonella / Q000276:immunology; D000685:Serum Amyloid A Protein / Q000276:immunology* / Q000302:isolation & purification; D012995:Solubility; D012996:Solutions; D014508:Urea / Q000494:pharmacology; D014867:Water
D000906:Antibodies; D000941:Antigens; D008075:Lipoproteins, HDL; D000685:Serum Amyloid A Protein; D012996:Solutions; D014867:Water; D014508:Urea