Growth-related changes of non-histone chromatin proteins from Kirkman-Robbins hepatoma.
1. Non-histone chromatin protein fractions NHCP1 and NHCP2 eluted from hydroxyapatite with 50 and 100 mM phosphate buffer (pH 6.8) from nuclei of Kirkman-Robbins hepatoma from 4th, 7th and 9th day of growth were analysed by one- and two-dimensional gel electrophoresis as well as Western blot technique in the presence of antibodies elicited against NHCP1, NHCP2 and dehistonized chromatin of hamster hepatoma and liver. 2. The presence of electrophoretically and immunologically specific components among NHCP1 and NHCP2 fractions during Kirkman-Robbins hepatoma growth was stated.
Kilianska
Z
Z
Department of Cytobiochemistry, University of Lódź, Poland.
Krajewska
W M
WM
Gaczynski
M
M
Marszalek
M
M
Klyszejko-Stefanowicz
L
L
D000818:Animals; D002467:Cell Nucleus / Q000032:analysis; D002843:Chromatin / Q000032:analysis; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D008114:Liver Neoplasms, Experimental / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008647:Mesocricetus
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone