Development of an avian calcitonin radioimmunoassay using synthetic chicken calcitonin as immunogen.
1. A sequential double antibody radioimmunoassay (RIA) has been developed using synthetic chicken calcitonin (CT) as antigen, tracer and standard. 2. The immunoassay has a minimum detection limit of 0.5 ng and effective dose (ED50) of 7 ng. Serial dilutions of chicken and turkey plasma were parallel to serial dilutions of CT standard. Extracts of chicken and turkey ultimobranchial glands caused parallel displacement of tracer similar to synthetic CT. 3. Primary antisera (anti-chicken CT) was raised in guinea pigs immunized with RIBI: animals treated with Freund's complete adjuvant failed to respond. 4. Chicken CT was determined to have a half-life of 60 sec in the turkey hen. Development of a homologous RIA for avian CT will allow studies to elucidate the role of this hormone in birds.
McMurtry
J P
JP
USDA-Agricultural Research Service, Beltsville Agricultural Research Center, MD 20705.
Steele
N C
NC
D000818:Animals; D002116:Calcitonin / Q000097:blood* / Q000276:immunology; D002645:Chickens / Q000097:blood; D006207:Half-Life; D011863:Radioimmunoassay; D012015:Reference Standards; D014422:Turkeys / Q000097:blood
D002116:Calcitonin