Binding activity of a murine anti-lipid A monoclonal antibody.
In this report we briefly describe an immunoglobulin G3 monoclonal antibody, 2G6/1H11, which binds purified lipid A from Salmonella minnesota and a lipid A precursor molecule derived from Salmonella typhimurium. 2G6/1H11 does not bind well to purified whole S. typhimurium lipopolysaccharide (LPS), S. minnesota LPS, LPS preparations from a series of S. minnesota rough mutants, or intact S. typhimurium bacteria. Thus, there are antigenic determinants in purified lipid A which are not exposed when lipid A is presented as part of the whole LPS molecule or intact bacteria.
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000915:Antibody Affinity; D000939:Epitopes / Q000276:immunology; D005260:Female; D006017:Glycolipids; D007074:Immunoglobulin G / Q000276:immunology; D008050:Lipid A / Q000031:analogs & derivatives / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D012475:Salmonella / Q000276:immunology*; D012486:Salmonella typhimurium / Q000276:immunology; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006017:Glycolipids; D007074:Immunoglobulin G; D008050:Lipid A; D008070:Lipopolysaccharides; C040142:lipid A precursors, bacterial