title: Partial purification of parathyrin from the corpuscles of Stannius (PCS) of the eel (Anguilla anguilla, L.).
abstract: It has been previously shown that the eel corpuscles of Stannius (CS) synthesize and secrete a substance (PCS) which is functionally and immunologically related to the mammalian parathyrin family. Purification of PCS, including anion-exchange chromatography, ODS C-18 reverse-phase HPLC, and affinity chromatography, showed that a biologically active peak, eluted in 32% acetonitrile, contains a 32- to 34-kDa protein which is 600-fold more potent than the crude extract is a test involving the hypocalcemic response in the CS-deprived eel. Specific immunoprecipitation of protein encoded by mRNA extracted from eel CS indicates that a 45-kDa precursor is involved in PCS synthesis. The hypothetical significance of a "large" parathyrin-like molecule in fish is suggested in relation to what is known about mammalian parathyrin gene expression.
authors.affiliation: Laboratoire de Physiologie Générale et Comparée du Muséum National d'Histoire Naturelle; U.A. 90 CNRS, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Milet
authors.forename: E
authors.initials: E
authors.lastname: Martelly
authors.forename: E
authors.initials: E
authors.lastname: Lopez
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004524:Eels / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D008715:Methionine / Q000378:metabolism; D010281:Parathyroid Hormone / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D010281:Parathyroid Hormone; D011506:Proteins; D012333:RNA, Messenger; D008715:Methionine; D000469:Alkaline Phosphatase