title: Efficacy of inactivated vaccine containing cyto-hemagglutinin against epidemic hemorrhagic fever in rabbits.
abstract: Using the Z-10 strain of epidemic hemorrhagic fever virus (EHFV) as seed, and the primary cell of Meriones unguiculatus kidney tissue as incubation cell, a propiolactone inactivated epidemic hemorrhagic fever (EHF) vaccine was prepared, according to a similar procedure required for the production of biological products such as the Japanese B encephalitis vaccine. Besides the EHFV antigen detected by ELISA or reversed passive hemagglutination test (RPHA) as were used for the formalin inactivated vaccine, higher titres (1:128-1:1024) of EHFV hemagglutinin antigen was also detected in this EHFV vaccine. Immunization with twice intramuscular injection of this vaccine produced high titred (1:20-1:160) neutralizing antibody and low titred (1:10-1:20) hemagglutination antibody, in addition to the immunofluorescence (IF) and reversed passive hemagglutination inhibition (RPHI) antibodies. These results indicated an apparent difference in the immunogenicity between the beta-propiolactone and formalin inactivated EHF vaccines. With the approval of the Ministry of Health, human test is now underway in this laboratory.
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Zhu
authors.forename: H Y
authors.initials: HY
authors.lastname: Tang
authors.forename: Y J
authors.initials: YJ
authors.lastname: Li
authors.forename: J Q
authors.initials: JQ
authors.lastname: Weng
authors.forename: G M
authors.initials: GM
authors.lastname: Fu
mesh_terms: D000818:Animals; D005260:Female; D006476:Orthohantavirus / Q000276:immunology*; D006389:Hemagglutinins, Viral / Q000276:immunology*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000517:prevention & control*; D008297:Male; D011817:Rabbits; D015164:Vaccines, Inactivated; D014765:Viral Vaccines
chemical_list: D006389:Hemagglutinins, Viral; D015164:Vaccines, Inactivated; D014765:Viral Vaccines