Turnover of sulphated macromolecules in the murine endolymphatic sac after long-term kanamycin treatment.
Radioactively labeled sulphur was injected into 12 mice of the NMRI strain 20 days after pretreatment with kanamycin. The animals were decapitated after intervals ranging from 2 minutes to 24 hours after injection. After a routine autoradiographic procedure, darkly stained silver grains were detected in the endolymphatic sac (ES) and its surroundings. One hour after injection, the grains were found in the surrounding blood vessels. Eleven hours later, maximal uptake was seen in the epithelial cells of the ES in the kanamycin-treated animals. Twenty-four hours after injection, a faint S35 uptake in the ES could still be detected. An increase in the ES activity, indicated by an increased number of free-floating cells and the secretion of a sulphur-containing intraluminal substance, did not occur. The spreading pattern of sulphur, as shown in this investigation, does not support the theory that waste products from the inner ear, transported to the ES by the longitudinal flow, produces an increased activity in the sac after long pretreatment with kanamycin. In 12 control animals, no difference in the distribution of labeling was observed, but there was an additional uptake in the free intraluminal cells which was not seen in the kanamycin group.
Hultcrantz
M
M
Department of Otorhinolaryngology, Karolinska Hospital, Stockholm, Sweden.
Nilsson
J
J
Bagger-Sjöbäck
D
D
D000818:Animals; D001345:Autoradiography; D004710:Endolymph / Q000187:drug effects*; D004712:Endolymphatic Sac / Q000187:drug effects*; D006025:Glycosaminoglycans / Q000378:metabolism*; D007612:Kanamycin / Q000633:toxicity*; D007761:Labyrinthine Fluids / Q000187:drug effects*; D051379:Mice; D008815:Mice, Inbred Strains; D013455:Sulfur / Q000378:metabolism; D014722:Vestibule, Labyrinth / Q000187:drug effects*
D006025:Glycosaminoglycans; D007612:Kanamycin; D013455:Sulfur