Antigen capture ELISA for HTLV I in cell culture.
Recent reports have shown that HTLV I infection is widespread and that HIV/HTLV I combined infections may accelerate the progression of seropositive patients towards AIDS. In order to obtain a tool for the detection and identification of HTLV I in cell culture we have developed an antigen-capture ELISA, which is based on the use of rabbit hyperimmune serum on solid phase and mouse monoclonal antibodies against p19 and p24 as tracers. This assay has a sensitivity of 50 ng/ml, it can be used to test cell-culture fluids and does not cross-react with any other known human retroviruses.
Bonfanti
C
C
Institute of Microbiology, University of Brescia, Italy.
Manca
N
N
Caruso
A
A
Gargiulo
F
F
Turano
A
A
D002460:Cell Line; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D015486:HTLV-I Antigens / Q000032:analysis*; D015490:HTLV-I Infections / Q000175:diagnosis*; D015368:Human T-lymphotropic virus 1 / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D012191:Retroviridae Proteins / Q000032:analysis*
D015486:HTLV-I Antigens; D012191:Retroviridae Proteins