Purification and characterization of a unique osteoinductive factor from bovine bone.
A unique protein that promotes ectopic osteoinduction in the rat has been isolated and characterized. Osteoinductive factor (OIF) was extracted from the organic matrix of bovine bone with 4 M guanidine HCl and purified by gel filtration, ion-exchange chromatography, affinity chromatography, and reversed phase high performance liquid chromatography. OIF is a glycoprotein with an apparent molecular mass of 22-28 kDa based on sodium dodecyl sulfate gel electrophoresis. Enzymatic or chemical deglycosylation of OIF reduces its mass to about 12 kDa with apparent loss of activity. OIF activity in the model used is substantially increased by addition of transforming growth factor (TGF)-beta 1 or TGF-beta 2, suggesting an important role for TGF-beta 1 and -2 in bone regeneration and repair. The N-terminal sequence of OIF has no homology to other reported proteins.
Bentz
H
H
Celtrix Laboratories, Collagen Corporation, Palo Alto, California 94303.
Nathan
R M
RM
Rosen
D M
DM
Armstrong
R M
RM
Thompson
A Y
AY
Segarini
P R
PR
Mathews
M C
MC
Dasch
J R
JR
Piez
K A
KA
Seyedin
S M
SM
D000595:Amino Acid Sequence; D000818:Animals; D001846:Bone Development / Q000187:drug effects; D001842:Bone and Bones / Q000187:drug effects / Q000502:physiology*; D002417:Cattle; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D006023:Glycoproteins / Q000302:isolation & purification*; D006133:Growth Substances / Q000302:isolation & purification* / Q000494:pharmacology; D036341:Intercellular Signaling Peptides and Proteins; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009924:Organ Culture Techniques; D051381:Rats; D011919:Rats, Inbred Strains; D015291:Transforming Growth Factors / Q000494:pharmacology
D006023:Glycoproteins; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; C512905:Ogn protein, rat; D015291:Transforming Growth Factors