Gastric mucosal damage induced in rats by intravenous administration of platelet-activating factor.
We examined the time courses for gastric mucosal hemodynamics and lipid peroxides after intravenous infusion of PAF (platelet-activating factor). The gastric mucosal hemodynamics were continuously monitored by laser-Doppler flowmetry and reflectance spectrophotometry during 20 min infusion of PAF and the subsequent 60 min. Gastric mucosal damage was assessed by microscopical scoring and measurement of thiobarbituric acid (TBA) reactants. Intravenous infusion of PAF caused macroscopical gastric mucosal damage, hemodynamic disturbance, a reduction in blood pressure and elevation of TBA reactants. These changes did not recover to control levels, even at 60 min after PAF infusion. The mucosal injury caused by the hemodynamic disturbance (congestion) was related to the elevation of TBA reactants, that reflect lipid peroxidation and oxygen-derived free radicals.
Binnaka
T
T
Third Department of Internal Medicine, Kansai Medical University, Osaka, Japan.
Yamaguchi
T
T
Hirohara
J
J
Hiramatsu
A
A
Mizuno
T
T
Sameshima
Y
Y
D000818:Animals; D005753:Gastric Mucosa / Q000098:blood supply / Q000187:drug effects* / Q000473:pathology; D006439:Hemodynamics; D007275:Injections, Intravenous; D008297:Male; D010972:Platelet Activating Factor / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013858:Thiobarbiturates; D013997:Time Factors
D010972:Platelet Activating Factor; D013858:Thiobarbiturates; C029684:thiobarbituric acid