Brief maternal deprivation of rats reduces hepatic mixed function oxidase activities.
Deprivation of pups from mother and sibs for 3 min daily from day 5 to day 41 of life reduced activities of 4 hepatic mixed function oxidases (MFO) expressed per mg protein in male rats compared to unhandled control rats. These decreases, though generally small, 22.4% and under, reached statistical significance for the substrates aminopyrine, benzphetamine and ethoxycoumarin. This handling procedure did not consistently affect the inductive response to phenobarbital. Previously ignored as a source of variability in response to xenobiotics, "handling" appears from these results to merit further investigation as such a factor in uninduced rats. Differences among rats in "handling" could contribute to large day-to-day variations in their metabolism of xenobiotics.
Vesell
E S
ES
Department of Pharmacology, Pennsylvania State University College of Medicine, Hershey 17033.
Heubel
F
F
Netter
K J
KJ
D000632:Aminopyrine / Q000378:metabolism; D000818:Animals; D001589:Benzphetamine / Q000378:metabolism; D001711:Biotransformation; D001835:Body Weight; D003374:Coumarins / Q000378:metabolism; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D006234:Handling, Psychological; D008297:Male; D008426:Maternal Deprivation; D008862:Microsomes, Liver / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D010078:Oxazines / Q000378:metabolism; D010634:Phenobarbital / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D003374:Coumarins; D010078:Oxazines; D000632:Aminopyrine; D001589:Benzphetamine; C017299:7-ethoxycoumarin; C044943:pentoxyresorufin; D006899:Mixed Function Oxygenases; D010634:Phenobarbital