Comparative studies of prostaglandin E2, phenylisopropyladenosine, clonidine and propranolol on lipolysis stimulated by methylisobutylxanthine and isoprenaline in hamster fat cells.
The antilipolytic effects of prostaglandin E2 (PGE2), phenylisopropyladenosine (PIA), clonidine (alpha-2 adrenoceptor agonist) and propranolol (beta- adrenoceptor antagonist) on lipolysis stimulated by methylisobutylxanthine (MIX) (2 x 10(-4)M) and isoprenaline (5 x 10(-7)M) were compared in hamster fat cells. PGE2, PIA and clonidine inhibited markedly lipolysis stimulated by MIX, and slightly that stimulated by isoprenaline. Whilst propranolol inhibited markedly lipolysis stimulated by isoprenaline and slightly that stimulated by MIX. These results indicate that the inhibitory activity of PGE2, adenosine and alpha-2-adrenergic receptor stimulation depends upon the presence of MIX, whereas that of the beta-adrenoceptor blockade does not.
Tan
S
S
Curtis-Prior
P B
PB
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D003000:Clonidine / Q000494:pharmacology*; D006224:Cricetinae; D015232:Dinoprostone; D007545:Isoproterenol / Q000494:pharmacology; D008053:Lipid Mobilization / Q000187:drug effects*; D008297:Male; D010660:Phenylisopropyladenosine / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology*; D011458:Prostaglandins E / Q000494:pharmacology*; D011956:Receptors, Cell Surface / Q000187:drug effects; D011983:Receptors, Purinergic
D011458:Prostaglandins E; D011956:Receptors, Cell Surface; D011983:Receptors, Purinergic; D010660:Phenylisopropyladenosine; D011433:Propranolol; D015232:Dinoprostone; D007545:Isoproterenol; D003000:Clonidine; D015056:1-Methyl-3-isobutylxanthine