Separation of collagens by capillary zone electrophoresis.
Collagen (types I, II, V, IX and XI) constituting polypeptide chains and their polymers and cyanogen bromide-cleaved peptides of collagen type I and type III were investigated by means of capillary zone electrophoresis. Separations were effected in 2.5 mM sodium tetraborate buffer in less than 15 min. A 50 cm x 0.1 mm I.D. fused-silica capillary was used. The separations were run at 18 kV per capillary. The results of the separation were monitored at 220 nm with an on-tube detection system. Using the Offord equation, relative retention times of cyanogen bromide cleavage fragments were plotted against M(2-3)/Z, where M is the molecular mass of a polypeptide and Z its valency. A linear relationship was observed. Collagen alpha-chains and their polymers were also satisfactorily resolved.
Deyl
Z
Z
Institute of Physiology, Czechoslovak Academy of Science, Prague.
Rohlicek
V
V
Adam
M
M
D003094:Collagen / Q000302:isolation & purification*; D003488:Cyanogen Bromide; D004586:Electrophoresis / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D007202:Indicators and Reagents
D007202:Indicators and Reagents; D003094:Collagen; D003488:Cyanogen Bromide