Arsonate-specific murine T cell clones. II. Delayed-type hypersensitivity induced by P-azobenzenearsonate-L-tyrosine (ABA-Tyr).
To study T cell idiotype expression at the functional level, we developed a hapten-specific delayed-type hypersensitivity (DTH) system by which we avoid the complication of anti-hapten antibody and which is specific only for the immunizing hapten, and not for conjugate specific determinants. Immunization with ABA-Tyr and challenge with ABA diazonium induced footpad swelling with the characteristics of DTH. Anti-ABA antibodies did not contribute to this reaction, as they were undetectable in mice immunized with ABA-Tyr. Furthermore, this ABA-Tyr-specific DTH was under Ir gene control identical to that reported for ABA-Tyr-specific lymphocyte proliferation. All mouse strains tested responded to ABA-Tyr except those of the b haplotype across the entire Ia region. In contrast, contact sensitivity induced by ABA diazonium was not under apparent Ir gene control, probably reflecting 1) different specificities of the induced T cells and 2) the production of anti-ABA antibodies that contribute to the footpad swelling via an Arthus reaction. Having shown that ABA-Tyr can induce T cells mediating DTH, we then examined ABA-Tyr-reactive T cell clones, propagated in vitro, for their ability to mediate DTH. Such clones elicited a response identical to that seen with in vivo immunization with respect to dose dependency, I-Ak restriction, and antigen specificity.
Morita
C T
CT
Goodman
J W
JW
Lewis
G K
GK
D000818:Animals; D001391:Azo Compounds / Q000276:immunology*; D002999:Clone Cells / Q000276:immunology; D000939:Epitopes; D005260:Female; D005802:Genes, MHC Class II; D006968:Hypersensitivity, Delayed / Q000209:etiology / Q000235:genetics / Q000276:immunology*; D007116:Immunization, Passive; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D013601:T-Lymphocytes / Q000276:immunology*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000276:immunology; D010132:p-Azobenzenearsonate / Q000031:analogs & derivatives / Q000276:immunology*
D001391:Azo Compounds; D000939:Epitopes; C000234:azobenzenearsonate-N-acetyl-L-tyrosine; D014443:Tyrosine; D010132:p-Azobenzenearsonate