[Development of a method of obtaining peroxidase conjugates with anti-immunoglobulin for immunoenzyme analysis].
The results of the successive stages of preparing peroxidase-labeled anti-immunoglobulins are presented. The schedules for the immunization of animals have been worked out with a view to prepare antisera to human gamma globulin and, subsequently, to isolate antibodies from them by different methods. The preparations obtained with the use of immunosorbent have proved to possess the highest serological activity. As the result of these investigations, a new method of binding, a modification of the glutaraldehyde method, has been proposed. The conjugates obtained by the proposed method are characterized by high serological activity, stability and good reproducibility.
Iankina
N F
NF
Vaneeva
L I
LI
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification; D006386:Hemagglutination Tests; D006801:Humans; D007106:Immune Sera / Q000302:isolation & purification; D007114:Immunization / Q000379:methods; D007124:Immunoenzyme Techniques; D007136:Immunoglobulins / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D007163:Immunosorbent Techniques; D010544:Peroxidases / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D011817:Rabbits; D013997:Time Factors; D005719:gamma-Globulins / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification
D000888:Antibodies, Anti-Idiotypic; D007106:Immune Sera; D007136:Immunoglobulins; D005719:gamma-Globulins; D010544:Peroxidases
Razrabotka metoda polucheniia kon'iugatov peroksidazy s antiimmuno-globulinom dlia immunofermentnogo analiza.