title: Detection of human coronavirus 229E in nasal washings using RNA:RNA hybridisation.
abstract: A method is described for the detection of human coronavirus 229E (HCV 229E) in nasal washings using RNA:RNA filter hybridisation. Volunteers were inoculated with HCV 229E, and daily nasal washings were collected. These washings were then examined for the presence of viral RNA using a single-stranded RNA probe. Nucleic acid hybridisation is shown to be a sensitive technique for the diagnosis of HCV 229E infections.
authors.affiliation: Institute of Virology, University of Würzburg, Federal Republic of Germany.
authors.forename: S
authors.initials: S
authors.lastname: Myint
authors.forename: S
authors.initials: S
authors.lastname: Siddell
authors.forename: D
authors.initials: D
authors.lastname: Tyrrell
mesh_terms: D003332:Coronaviridae / Q000235:genetics / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D006801:Humans; D009296:Nasal Cavity / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D015347:RNA Probes
chemical_list: D015347:RNA Probes