Immobilization of native and denatured DNA on Sephadex G200.
An efficient method for the immobilization of DNA on Sephadex G200 in the presence of water soluble carbodiimide is described and investigated in this paper. An increase in the extent of binding was observed when the incubation temperature of the DNA-Sephadex mixture was changed. It was found that native DNA immobilized to Sephadex with higher efficiency than denatured DNA. However, the stability of native DNA-Sephadex complex was about the same as that of denatured DNA-Sephadex. The size of DNA released by DNA-Sephadex after incubation of a suspension of the complex was the same as that of the DNA used for immobilization. The binding mechanism of DNA to Sephadex is discussed.
Mykoniatis
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D000818:Animals; D002234:Carbodiimides; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D002846:Chromatography, Affinity / Q000379:methods; D004247:DNA; D003911:Dextrans; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation; D013329:Structure-Activity Relationship; D013696:Temperature; D013950:Thymus Gland
D002234:Carbodiimides; D003911:Dextrans; D004247:DNA; C025614:sephadex