Effect of anthracycline antibiotics on the reconstituted mitochondrial tricarboxylate carrier.
The effect of anthracycline antibiotics on the activity of the partially purified and reconstituted tricarboxylate carrier system of the rat liver mitochondria was studied. It was found that the citrate/citrate exchange activity is inhibited by Br-daunomycin and with less potency by doxorubicin, daunomycin, epirubicin and idarubicin. The inhibition of the citrate transport activity is concentration and time-dependent. Cardiolipin protects against the inhibition by Br-daunomycin and the reconstituted citrate transport activity depends upon the ratio of cardiolipin/Br-daunomycin.
Stipani
I
I
Department of Pharmaco-Biology, University of Bari, Italy.
Capalbo
M I
MI
Zara
V
V
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D002352:Carrier Proteins / Q000378:metabolism*; D002951:Citrates / Q000378:metabolism; D003630:Daunorubicin / Q000031:analogs & derivatives / Q000494:pharmacology; D004317:Doxorubicin / Q000494:pharmacology; D015251:Epirubicin / Q000494:pharmacology; D015255:Idarubicin / Q000494:pharmacology; D007700:Kinetics; D008930:Mitochondria, Liver / Q000378:metabolism*; D051381:Rats
D000903:Antibiotics, Antineoplastic; D002352:Carrier Proteins; D002951:Citrates; C020723:citrate-binding transport protein; D015251:Epirubicin; C059433:14-bromodaunorubicin; D004317:Doxorubicin; D015255:Idarubicin; D003630:Daunorubicin