Phenotypic and cytogenetic characterization of a cell line derived from primary prostatic carcinoma.
A new cell line, PPC-I, has been established from a specimen obtained from a patient with a poorly differentiated adenocarcinoma of the prostate. This is the first line of its type derived from a primary prostatic tumor site. PPC-I cells have become immortalized in culture, exhibit transformation parameters including relaxed growth factor requirements and anchorage-independent growth, and are highly tumorigenic in nude mice. Cytogenetic studies by G-banding revealed a grossly abnormal karyotype, with a modal chromosome number of 84, multiple marker chromosomes including both homogeneously staining regions and double minutes and clonal loss of chromosomes 3, 5, 10, 15 and Y.
Brothman
A R
AR
Department of Microbiology/Immunology, Eastern Virginia Medical School, Norfolk 23501.
Lesho
L J
LJ
Somers
K D
KD
Wright
G L
GL
Merchant
D J
DJ
D000368:Aged; D000818:Animals; D002277:Carcinoma / Q000473:pathology*; D002871:Chromosome Banding; D004273:DNA, Neoplasm / Q000235:genetics; D004848:Epithelium / Q000473:pathology; D005819:Genetic Markers; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011471:Prostatic Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D005819:Genetic Markers