Action of mycosubtilin on erythrocytes and artificial membranes.
Mycosubtilin has a strong lytic action upon erythrocytes. The haemolytic effect of the antibiotic is inhibited by free cholesterol as well as cholesterol included in liposomes of phosphatidylcholine-cholesterol. The antibiotic binding on artificial membranes was studied with radioactive bilayer vesicles of dipalmitoylphosphatidylcholine (DPPC), and DPPC-cholesterol:mycosubtilin destroyed both kinds of vesicles. The inhibition of the haemolytic effect of mycosubtilin by liposomes is a result of an interaction which diminishes the concentration of free cholesterol.
Besson
F
F
Laboratoire de Biochimie Microbienne (CNRS UA 1176), Université Claude Bernard, Villeurbanne, France.
Quentin
M J
MJ
Michel
G
G
D015060:1,2-Dipalmitoylphosphatidylcholine; D000935:Antifungal Agents / Q000494:pharmacology; D002784:Cholesterol / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D008074:Lipoproteins / Q000494:pharmacology; D008081:Liposomes; D008567:Membranes, Artificial
D000935:Antifungal Agents; D008074:Lipoproteins; D008081:Liposomes; D008567:Membranes, Artificial; C012448:mycosubtiline; D015060:1,2-Dipalmitoylphosphatidylcholine; D002784:Cholesterol