Some factors affecting optimal differential staining of sister-chromatids in vivo in the fish Nothobranchius rachowi.
In the past few years, routine studies of SCE induction in vivo in fish have been hampered by unreliable SCD techniques. This paper presents a number of modifications of the SCD technique in vivo in Nothobranchius rachowi. Major improvements were obtained by BrdU incorporation from aqueous solutions, short intervals between preparation and staining of slides and post-treatment with HCl. These improvements resulted in a highly reliable SCD procedure in Nothobranchius with a low level base-line SCE frequency (0.90 SCE/metaphase, 0.059 SCE/chromosome). Further research is now directed at gathering additional data on base-line SCE frequencies, establishing the sensitivity of the assay for aqueous solutions of known mutagens, and defining an experimental set-up for optimal statistical evaluation.
van de Kerkhoff
J F
JF
van der Gaag
M A
MA
D000818:Animals; D006690:Bisbenzimidazole; D001973:Bromodeoxyuridine; D002455:Cell Division; D005399:Fishes / Q000235:genetics*; D008677:Metaphase; D012854:Sister Chromatid Exchange; D013194:Staining and Labeling / Q000379:methods*; D013997:Time Factors
D001973:Bromodeoxyuridine; D006690:Bisbenzimidazole