title: Evaluation of monomethoxypolyethyleneglycol derivatized superoxide dismutase in blood and evidence for its binding to blood cells.
abstract: A method to evaluate in blood free superoxide dismutase (SOD) and SOD covalently bound to monomethoxypolyethylene glycol (MPEG-5000) is reported. The method takes advantage of gel filtration and cationic exchange chromatography to remove substances present in plasma which can interfere with the SOD enzymatic assay. Using this methodology, it was demonstrated that, contrary to free SOD, MPEG-SOD, when added to blood, was not completely recovered in plasma. Binding of MPEG-SOD to blood cell components takes place involving interactions of MPEG chains with cell membranes.
authors.affiliation: Dipartimento Di Scienze Farmaceutiche, Università Di Padova, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Caliceti
authors.forename: O
authors.initials: O
authors.lastname: Schiavon
authors.forename: A
authors.initials: A
authors.lastname: Mocali
authors.forename: F M
authors.initials: FM
authors.lastname: Veronese
mesh_terms: D000818:Animals; D001773:Blood Cells / Q000201:enzymology*; D002417:Cattle; D004912:Erythrocytes / Q000201:enzymology; D066298:In Vitro Techniques; D011092:Polyethylene Glycols / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000097:blood* / Q000302:isolation & purification
chemical_list: D011092:Polyethylene Glycols; C028210:monomethoxypolyethylene glycol; D013482:Superoxide Dismutase