Characterization of the thymus leukemia (TL) product encoded by the BALB/c T3c gene by DNA-mediated gene transfer. Comparison to the T13c product and BALB/c leukemia TL.
Using DNA-mediated gene transfer, we have studied the TL protein products encoded by both the T3c and T13c BALB/c genes. Biochemically, the proteins differed in their m.w. and pI points; serologically, although both molecules were recognized by TL alloantiserum, only the T13c protein was recognized by monoclonal TL antibodies. Interestingly, both proteins were serologically and immunochemically recognized by leukemia-specific TL.4 antiserum. The quantity of cell surface T13 was significantly greater than T3 possibly due to the less efficient splicing of T3 transcripts in the L cell nucleus; both genes directed the synthesis of cytoplasmic RNA containing an unspliced intron 3 as assessed by S1 analysis. In toto, the results suggest that T3c is similar or perhaps identical with the novel TL product previously identified on the surface of certain x-ray-induced BALB/c leukemias.
Chorney
M J
MJ
Department of Microbiology and Immunology, Pennsylvania State University College of Medicine, Hershey, PA 17033.
Mashimo
H
H
Bushkin
Y
Y
Nathenson
S G
SG
D000818:Animals; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D052138:Genes, Neoplasm; D015458:Leukemia, T-Cell / Q000235:genetics / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000302:isolation & purification*; D051379:Mice; D008807:Mice, Inbred BALB C; D009363:Neoplasm Proteins / Q000235:genetics / Q000302:isolation & purification*; D010641:Phenotype; D011233:Precipitin Tests; D014162:Transfection
D004273:DNA, Neoplasm; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins; C028388:thymus-leukemia antigens