title: Sidedness of phosphatidylcholine-synthesizing enzymes in rat brain microsomal vesicles.
abstract: The sidedness of CDP-choline:1,2-diradylglycerol choline phosphotransferase (EC 2.7.8.2) and of the choline base-exchange activity has been studied in rat brain microsomal vesicles. Proteases (trypsin and pronase) and mercury-dextran have been used as reagents for membrane surface components. All of them could inactivate both enzymes to a good extent, without affecting the morphology or the permeability to sucrose of the vesicles. It is therefore concluded that CDP-choline:1,2-diradylglycerol choline phosphotransferase and the choline base-exchange activity are localized on the outer surface of rat brain microsomal vesicles.
authors.forename: G
authors.initials: G
authors.lastname: Arienti
authors.forename: L
authors.initials: L
authors.lastname: Corazzi
authors.forename: L
authors.initials: L
authors.lastname: Freysz
authors.forename: L
authors.initials: L
authors.lastname: Binaglia
authors.forename: R
authors.initials: R
authors.lastname: Roberti
authors.forename: G
authors.initials: G
authors.lastname: Porcellati
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology*; D002729:Chloromercuribenzoates / Q000494:pharmacology; D002794:Choline / Q000378:metabolism; D003911:Dextrans / Q000494:pharmacology; D002798:Diacylglycerol Cholinephosphotransferase / Q000378:metabolism*; D008628:Mercury / Q000494:pharmacology; D008861:Microsomes / Q000201:enzymology; D009941:Organomercury Compounds; D010713:Phosphatidylcholines / Q000096:biosynthesis*; D010770:Phosphotransferases / Q000378:metabolism*; D011402:Pronase / Q000378:metabolism; D013997:Time Factors; D014357:Trypsin / Q000378:metabolism; D020245:p-Chloromercuribenzoic Acid
chemical_list: D002729:Chloromercuribenzoates; D003911:Dextrans; D009941:Organomercury Compounds; D010713:Phosphatidylcholines; C043248:mercury-dextran; D020245:p-Chloromercuribenzoic Acid; D010770:Phosphotransferases; D002798:Diacylglycerol Cholinephosphotransferase; D014357:Trypsin; D011402:Pronase; D008628:Mercury; D002794:Choline