The effects of acute and chronic exposure to ethanol on glucose uptake by rat jejunal brush-border membrane vesicles.
Rats were pair-fed for 28-32 days a nutritionally adequate liquid control diet or an isocaloric diet containing ethanol (5% v/v). Brush-border membrane vesicles were prepared from jejunal mucosa and Na+-dependent D-glucose accumulation was measured using sugar concentrations of 0.2-1.0 mmol l-1. In separate experiments the effects of acute ethanol exposure on glucose uptake by vesicles prepared from chow-fed rats were determined. Chronic ethanol ingestion enhanced Na+-gradient-dependent glucose uptake. In contrast, incubation of vesicles with alcohol (4% v/v), reduced active uptake of the sugar.
al-Balool
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Department of Physiology, Royal Free Hospital School of Medicine, London.
Debnam
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D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D000431:Ethanol / Q000633:toxicity*; D005947:Glucose / Q000378:metabolism*; D066298:In Vitro Techniques; D007583:Jejunum / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008871:Microvilli / Q000187:drug effects / Q000378:metabolism; D010539:Permeability; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000378:metabolism
D000431:Ethanol; D012964:Sodium; D005947:Glucose