Human follicular fluid adverses hamster spermatozoa motility.
To determine the optimal conditions for in vitro spermatozoa vitality, human and hamster spermatozoa were incubated at 37 degrees C in T6 medium supplemented with different biologic fluids (10% v/v). The fluids tested were human serum (HUS), hamster serum (HAS), and human follicular fluid (HUF). After incubation the spermatozoa were investigated for their qualitative and quantitative motility. Human spermatozoa maintained a good vitality in all fluids tested (approximately 25% motility after 18-h incubation). The hamster spermatozoa had after an incubation of 4 h a motility of 28.4% in HUS, 14.2% in HAS, and 2.2% in HUF. The quality of the motility was also extremely low in HUF, whereas it was adequate in HUS and in HAS. The presence of species-specific substances in mammalian follicular fluid is discussed.
Wetzels
A
A
Division of Endocrinology and Reproduction, Catholic University, Nijmegen, The Netherlands.
Goverde
H J
HJ
Bastiaans
L A
LA
Rolland
R
R
D000818:Animals; D001790:Blood Physiological Phenomena; D006224:Cricetinae; D005260:Female; D015571:Follicular Fluid / Q000502:physiology*; D006801:Humans; D008297:Male; D008647:Mesocricetus; D013081:Sperm Motility / Q000502:physiology*