The functional association of Lyt antigens with lymphokine production.
The effects of anti-Lyt antibodies on secretion of lymphotoxin, macrophage-activating factor and interferon were studied. Anti-Lyt-1 antibodies added to primary mixed lymphocyte reactions (MLR) significantly enhanced lymphokine secretion, whereas anti-Lyt-2 antibodies had no effect in primary responses. However, when added to secondary MLR, anti-Lyt-1 antibodies manifested moderate enhancing effect on lymphokine levels, whereas anti-Lyt-2 antibodies markedly reduced lymphokine secretion. These results suggested that T cells of different subsets produced lymphokines during primary and secondary responses. The Lyt phenotype of T cells involved in lymphokine secretion and the effects of anti-Lyt antibodies on selected T-cell subsets were therefore studied. It was demonstrated that the majority of lymphokine producing cells during primary MLR reside within the Lyt-1+2- population, which was potentiated by anti-Lyt-1 antibodies, but unaffected by anti-Lyt-2 antibodies. However, during secondary MLR, a considerable proportion of lymphokines was produced by the Lyt-1-2+ population, which was blocked by anti-Lyt-2 antibodies. Lymphokine production induced by concanavalin A, in contrast to that induced by alloantigens, was unaffected by anti-Lyt-2 antibodies. The implication of these results on the role of Lyt molecules in T-cell functions is discussed.
Hollander
N
N
Shepshalovich
J
J
D000818:Animals; D000950:Antigens, Ly / Q000276:immunology; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D007372:Interferons / Q000378:metabolism; D007518:Isoantibodies / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism*; D008222:Lymphokines / Q000378:metabolism*; D008233:Lymphotoxin-alpha / Q000378:metabolism; D016215:Macrophage-Activating Factors; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL
D000950:Antigens, Ly; D007518:Isoantibodies; D008222:Lymphokines; D008233:Lymphotoxin-alpha; C029805:Lyt antibodies; D016215:Macrophage-Activating Factors; D003208:Concanavalin A; D007372:Interferons