In vitro development of metacyclic Trypanosoma simiae derived from bloodstream trypomastigotes.
A stock of Trypanosoma simiae was transformed into procyclic forms at 26 C in a semi-defined maintenance medium. After transformation, the trypanosomes were maintained in a modified Eagle's MEM medium. On day 35 of cultivation, epimastigotes attached to the bottom of the culture flask. From day 44 onwards, metacyclic-like trypanosomes were observed. Subcutaneous injections into pigs of trypanosome suspensions obtained from cultures on day 10 were not infective, whereas culture-derived metacyclics (days 44, 63 and 69) were highly pathogenic.
Zweygarth
E
E
Kenya Trypanosomiasis Research Institute, Kikuyu, Kenya.
Gumm
I D
ID
Gray
M A
MA
Cheruiyot
J K
JK
Webster
P
P
Kaminsky
R
R
D000818:Animals; D003470:Culture Media; D008854:Microscopy, Electron; D013552:Swine; D013553:Swine Diseases / Q000469:parasitology*; D014345:Trypanosoma / Q000254:growth & development* / Q000472:pathogenicity / Q000648:ultrastructure; D014353:Trypanosomiasis, African / Q000469:parasitology / Q000662:veterinary*
D003470:Culture Media