Characterization of adenosine-bovine serum albumin immunogen and its antibodies.
Antibodies to adenosine were elicited in rabbits using a conjugate of adenosine (periodate oxidized) and bovine serum albumin as immunogen. The conjugate was found to be of a higher molecular size than the original protein, apparently due to the cross-linking of albumin molecules by the dialdehydes generated during the periodate oxidation of adenosine. It produced high titres of antibodies, which were 400-fold more reactive to adenosine-trialcohol than to adenosine, when measured by a nitrocellulose filter assay. In addition, a specific elution procedure for the isolation of hapten-specific antibodies is described.
Ali
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MA
Jacob
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TM
D000241:Adenosine / Q000031:analogs & derivatives / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D000918:Antibody Specificity; D002846:Chromatography, Affinity; D000939:Epitopes; D006241:Haptens; D008970:Molecular Weight; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000276:immunology; D013329:Structure-Activity Relationship
D000906:Antibodies; D000939:Epitopes; D006241:Haptens; D012710:Serum Albumin, Bovine; D000241:Adenosine