Suppression of murine IgE responses with amino acid polymer/allergen conjugates. I. Preparation of poly-(N-methylglycine)/grass pollen extract conjugates using 4-(methylmercapto)phenyl-succinimidyl succinate as coupling reagent.
A procedure utilising the latent activating potential of the 4-(methylmercapto)phenyl ester group has been developed for the controlled, reproducible preparation of macromolecular conjugates. This ester, as part of a succinyl-bridging group, was used to couple the water-soluble, amino acid polymer, poly-(N-methylglycine) (polysarcosine), via its N-terminal secondary amine, with the nucleophilic components of the aqueous extract of a mixture of grass pollens. The products exhibit a reproducible, antigen-specific suppressive effect on the in vivo synthesis of IgE in mice.
Whittall
N
N
Moran
D M
DM
Wheeler
A W
AW
Cottam
G P
GP
D000485:Allergens / Q000276:immunology*; D000818:Animals; D007073:Immunoglobulin E / Q000276:immunology*; D007165:Immunosuppression Therapy; D008024:Ligands; D046911:Macromolecular Substances; D051379:Mice; D010455:Peptides / Q000276:immunology*; D012521:Sarcosine / Q000031:analogs & derivatives* / Q000276:immunology; D013388:Succinimides
D000485:Allergens; D008024:Ligands; D046911:Macromolecular Substances; D010455:Peptides; D013388:Succinimides; C015475:polysarcosine; D007073:Immunoglobulin E; C044918:4-(methylmercapto)phenyl succinimidyl succinate; D012521:Sarcosine