Metabolism of 3-methylcholanthrene in rat liver cytosol.
The present study investigates the metabolism of the potent carcinogen 3-methylcholanthrene in rat liver cytosol preparations. Three metabolites of 3-methylcholanthrene were characterized by HPLC and GC/MS analysis. These metabolites were identified as 1-hydroxy-3-methylcholanthrene, 1-keto-3-methylcholanthrene and cholanthrene. The results of the present study, taken together with earlier studies, suggests that the first step in the metabolic activation of 3-methylcholanthrene is hydroxylation at the 1-position, the most easily oxidized reactive center in the molecule.
Myers
S R
SR
Department of Pharmacology, Experimental Cancer Research Laboratory, Lexington, KY.
Blake
J W
JW
Flesher
J W
JW
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003600:Cytosol / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006900:Hydroxylation; D008099:Liver / Q000378:metabolism*; D008297:Male; D008748:Methylcholanthrene / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D008748:Methylcholanthrene