Mammalian single-stranded DNA binding proteins and heterogeneous nuclear RNA proteins have common antigenic determinants.
Antibodies were raised in rabbit against a pure subset of calf thymus single-stranded DNA binding proteins (ssDBPs) and purified by affinity chromatography on antigen-Sepharose. In Western blot experiments these antibodies were shown to react to the same extent with the whole family of bovine ssDBPs, as well as with ssDBPs from HeLa cells. When used to stain total cell extracts from both calf thymus and HeLa cells the antibodies reacted only with bands corresponding to the ssDBPs and with a set of bands of higher molecular weight, whose electrophoretic pattern matched that of the 40S hnRNP core proteins. In effect we observed that purified 40S hnRNP core proteins from HeLa cells were strongly reactive with the antibodies. Moreover after partial tryptic digestion HeLa cells ssDBPs and hnRNPs produced immunoreactive fragments of the same molecular weight and isoelectric point. Extensive structural homologies can thus be evidenced between these two classes of proteins, which share the property of selective binding to single-stranded nucleic acids.
Valentini
O
O
Biamonti
G
G
Pandolfo
M
M
Morandi
C
C
Riva
S
S
D000818:Animals; D002417:Cattle; D003429:Cross Reactions; D004277:DNA, Single-Stranded / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000276:immunology*; D000939:Epitopes; D005260:Female; D006367:HeLa Cells; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D006801:Humans; D010446:Peptide Fragments / Q000032:analysis; D012261:Ribonucleoproteins / Q000276:immunology*; D013950:Thymus Gland
D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D000939:Epitopes; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D010446:Peptide Fragments; D012261:Ribonucleoproteins