Lack of informative HLA restriction fragment length polymorphisms in autoimmune chronic active hepatitis.
Autoimmune chronic active hepatitis (CAH) is strongly associated with HLA-B8, DR3. Accordingly, DNA was isolated from 10 HLA-B8, DR3 control subjects and 11 patients with autoimmune CAH and analyzed for informative restriction fragment length polymorphisms using HLA-DQ beta and DR beta probes, and six enzymes, Hinc II, Bgl II, Bam HI, Rsa I, Taq I and Msp I. None of the polymorphic fragments demonstrable was discriminatory for autoimmune CAH. A genetic polymorphism in the HLA-B8, DR3 region predisposing to autoimmune CAH may not have been detecting owing to an insufficient number of probes or enzymes used, or alternatively HLA-DR3 is predisposing by an effect on immune regulation or suppression.
Eckardt
G S
GS
Clinical Research Unit, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia.
O'Brien
R M
RM
Mackay
I R
IR
De Martinis
L
L
Tait
B D
BD
D000328:Adult; D001327:Autoimmune Diseases / Q000235:genetics*; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D004587:Electrophoresis, Agar Gel; D005260:Female; D015795:HLA-B8 Antigen / Q000235:genetics*; D015803:HLA-DR3 Antigen / Q000235:genetics*; D006239:Haplotypes / Q000235:genetics; D006521:Hepatitis, Chronic / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length
D015342:DNA Probes; D015795:HLA-B8 Antigen; D015803:HLA-DR3 Antigen; D004247:DNA