Isolation of turbot (Scophthalmus maximus) vitellogenin by high-performance anion-exchange chromatography.
The use of high-performance anion-exchange chromatography on a Mono Q column for isolation of a glycolipophosphoprotein, vitellogenin, from turbot plasma has been evaluated. The method is an effective, rapid one-step procedure, which gives a pure preparation of vitellogenin as assessed by electrophoresis, [32P]orthophosphate incorporation and amino acid composition.
Silversand
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Department of Zoophysiology, University of Göteborg, Sweden.
Haux
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D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001345:Autoradiography; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005412:Flatfishes; D010710:Phosphates / Q000378:metabolism; D014819:Vitellogenins / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism
D000596:Amino Acids; D010710:Phosphates; D014819:Vitellogenins