Islet cell isolation in experimental d,l-ethionine pancreatitis in dogs.
Bulk isolation of islets of Langerhans for biochemical studies or transplantation has generally been associated with significant or even prohibitive contamination by acinar tissue. High-purity islet preparations are associated with an extremely low yield. The acinar tissue can be ablated with d,l-ethionine, but previously, this tactic has been restricted to rodents because of toxicity problems in larger animals. A dosage regimen in dogs which reduces amylase content of the pancreas to 0.3% of normal with complete preservation of insulin content is reported. Ductal perfusion with collagenase permits the recovery of 45% of the islet cell mass as determined by extractable insulin. These islets prove functional in in vitro perifusion studies and in allografts.
Cobb
L F
LF
Marincola
F
F
Horaguchi
A
A
Azumi
N
N
Merrell
R C
RC
D000681:Amylases / Q000032:analysis; D000818:Animals; D002469:Cell Separation / Q000379:methods; D004285:Dogs; D005001:Ethionine / Q000494:pharmacology*; D006651:Histocytochemistry; D007328:Insulin / Q000032:analysis; D007515:Islets of Langerhans / Q000032:analysis / Q000166:cytology / Q000187:drug effects*; D010179:Pancreas / Q000187:drug effects* / Q000473:pathology; D010195:Pancreatitis / Q000139:chemically induced / Q000473:pathology
D007328:Insulin; D000681:Amylases; D005001:Ethionine