Structural analysis of the gene for human acidic fibroblast growth factor.
Genomic clones derived from the gene for human acidic fibroblast growth factor (aFGF) have been isolated. Nucleotide sequence analysis of these clones revealed that the coding region of the human aFGF gene is interrupted by two introns, located at precisely homologous locations to introns in four other members of the FGF gene family, strongly indicating a common evolutionary origin for these genes. Northern blot analyses of the multiple aFGF transcripts found in serum-stimulated human foreskin fibroblasts indicated that the aFGF gene also contains a third intron, lying in the 5' untranslated region.
Mergia
A
A
California Biotechnology Inc., Mountain View 94043.
Tischer
E
E
Graves
D
D
Tumolo
A
A
Miller
J
J
Gospodarowicz
D
D
Abraham
J A
JA
Shipley
G D
GD
Fiddes
J C
JC
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002478:Cells, Cultured; D003001:Cloning, Molecular; D005346:Fibroblast Growth Factors / Q000235:genetics*; D015723:Gene Library; D005796:Genes; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D015347:RNA Probes; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D012867:Skin / Q000378:metabolism
D015345:Oligonucleotide Probes; D015347:RNA Probes; D005346:Fibroblast Growth Factors