title: Autoreactive T-cell lines specific for mouse thyroglobulin.
abstract: Autoreactive T-cell specific for mouse thyroglobulin have been established and characterized. These Lyt 1+ T cells proliferated specifically in response to thyroglobulin presented by syngeneic irradiated spleen cells. The antigen-presenting cell requirements of these autoreactive T cells appeared to be the same as those for foreign antigen (PPD) reactive T cells. All lines tested required antigen-presenting cells compatible at the I-A subregion of the H-2 complex. Both T-cell types responded to antigen presented by peritoneal cells and splenic dendritic cells, but only gave optimal responses when whole spleen cells were used. The cross-reactivity patterns of responses to mouse, rat, pig and human thyroglobulins indicated that at least two different epitopes could be recognized by the autoreactive T cells. Furthermore, these epitopes appeared to be different from those recognized by the majority of serum autoantibodies to mouse thyroglobulin.
authors.forename: B R
authors.initials: BR
authors.lastname: Champion
authors.forename: A M
authors.initials: AM
authors.lastname: Varey
authors.forename: D
authors.initials: D
authors.lastname: Katz
authors.forename: A
authors.initials: A
authors.lastname: Cooke
authors.forename: I M
authors.initials: IM
authors.lastname: Roitt
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D001323:Autoantibodies / Q000276:immunology; D002460:Cell Line; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology; D006183:H-2 Antigens / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007376:Interleukin-2; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008938:Mitosis; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013954:Thyroglobulin / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D000939:Epitopes; D006183:H-2 Antigens; D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2; D013954:Thyroglobulin