title: Major intrinsic polypeptide (MIP26K) from lens membrane: reconstitution into vesicles and inhibition of channel forming activity by peptide antiserum.
abstract: Bovine and human lens membrane, when reconstituted into lipid vesicles containing oxidized cytochrome C, will mediate the transmembrane passage of ascorbate into the vesicles, where the reduction of cytochrome C is measured spectrophotometrically. This channel forming activity is specifically inhibited by antiserum made against a synthetic octapeptide near the C-terminus of MIP26K. Together, these studies describe a direct and more sensitive assay system for measurement of channel-forming activity of MIP26K, and suggest that the C-terminus of this molecule may be particularly important in the regulation of channel formation.
authors.forename: M
authors.initials: M
authors.lastname: Gooden
authors.forename: D
authors.initials: D
authors.lastname: Rintoul
authors.forename: M
authors.initials: M
authors.lastname: Takehana
authors.forename: L
authors.initials: L
authors.lastname: Takemoto
mesh_terms: D000818:Animals; D020346:Aquaporins; D001205:Ascorbic Acid / Q000494:pharmacology; D001923:Brain Chemistry; D002417:Cattle; D003574:Cytochrome c Group / Q000378:metabolism; D005136:Eye Proteins / Q000276:immunology / Q000378:metabolism*; D005288:Ferredoxins / Q000494:pharmacology; D007106:Immune Sera; D007473:Ion Channels / Q000378:metabolism*; D007908:Lens, Crystalline / Q000032:analysis*; D008081:Liposomes / Q000378:metabolism; D008562:Membrane Glycoproteins
chemical_list: D020346:Aquaporins; D003574:Cytochrome c Group; D005136:Eye Proteins; D005288:Ferredoxins; D007106:Immune Sera; D007473:Ion Channels; D008081:Liposomes; D008562:Membrane Glycoproteins; C030328:aquaporin 0; D001205:Ascorbic Acid