title: Temperature-induced changes in the coenzyme environment of D-amino acid oxidase revealed by the multiple decays of FAD fluorescence.
abstract: A temperature-dependent change in the microenvironment of the coenzyme, FAD, of D-amino acid oxidase was investigated by means of steady-state and picosecond time-resolved fluorescence spectroscopy. Relative emission quantum yields from FAD bound to D-amino acid oxidase revealed the temperature transition when concentration of the enzyme was lowered. The observed fluorescence decay curves were well described with four-exponential decay functions. The amplitude of the shortest lifetime (tau 0), approximately 25 ps, was always negative, which indicates that the fluorescence of D-amino acid oxidase at approximately 520 nm appears after a metastable state of the excited isoalloxazine decays. The other components with positive amplitudes were assigned to dimer or associated forms of the enzyme, monomer, and free FAD dissociated from the enzyme. Ethalpy and entropy changes of intermediate states in the quenching processes were evaluated according to the absolute rate theory. The temperature transition was much more pronounced in the monomer than in the dimer or associated forms of the enzyme.
authors.affiliation: Mie Nursing College, Tsu, Japan.
authors.forename: F
authors.initials: F
authors.lastname: Tanaka
authors.forename: N
authors.initials: N
authors.lastname: Tamai
authors.forename: I
authors.initials: I
authors.lastname: Yamazaki
authors.forename: N
authors.initials: N
authors.lastname: Nakashima
authors.forename: K
authors.initials: K
authors.lastname: Yoshihara
mesh_terms: D000818:Animals; D001665:Binding Sites; D003605:D-Amino-Acid Oxidase / Q000378:metabolism*; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D011789:Quantum Theory; D013050:Spectrometry, Fluorescence / Q000379:methods; D013552:Swine; D013816:Thermodynamics
chemical_list: D005182:Flavin-Adenine Dinucleotide; D003605:D-Amino-Acid Oxidase