Ionic basis of salt-induced receptor potential in frog taste cells.
1. The ionic basis of the receptor potential elicited by salt stimuli in a frog taste cell was studied with intracellular microelectrodes and lingual artery perfusion. 2. The amplitudes of the receptor potentials induced by salts were decreased by 32-60% when interstitial Na+ and Ca2+ were replaced with choline+, tetramethylammonium+ and tetraethyl-ammonium+. 3. After removal of Na+ and Ca2+ from both interstitial and superficial fluids, the reversal potentials of NaCl induced receptor potentials changed depending upon the stimulus concentrations. 4. These results indicate that the direct influx of Na+ across the receptor membrane, as well as the influx of interstitial Na+ across the basolateral membrane, occurs during NaCl stimulation.
Miyamoto
T
T
Department of Physiology, Nagasaki University School of Dentistry, Japan.
Okada
Y
Y
Sato
T
T
D000818:Animals; D002712:Chlorides / Q000494:pharmacology; D008094:Lithium / Q000494:pharmacology; D018021:Lithium Chloride; D008564:Membrane Potentials / Q000187:drug effects; D011189:Potassium Chloride / Q000494:pharmacology*; D011892:Rana catesbeiana; D011984:Sensory Receptor Cells / Q000187:drug effects* / Q000502:physiology; D012965:Sodium Chloride / Q000494:pharmacology*; D012966:Sodium Cyanide / Q000494:pharmacology; D013649:Taste / Q000502:physiology*; D014059:Tongue / Q000166:cytology*
D002712:Chlorides; D012965:Sodium Chloride; D011189:Potassium Chloride; D008094:Lithium; D018021:Lithium Chloride; D012966:Sodium Cyanide