Major acute-phase alpha(1)protein in the rat: structure, molecular cloning, and regulation of mRNA levels.
Rat major acute-phase alpha(1)protein (MAP) was characterized by determining its secondary structure, ligand binding and partial amino acid sequence. A cDNA clone expressing MAP and coding for the entire mature protein was isolated from a cDNA library in E. coli prepared from rat liver mRNA. By hybridization to nick translated cDNA, mRNA for MAP was found only in liver, where it increased 17-fold during acute inflammation. Constant proportions of rates of leucine incorporation into MAP over mRNA levels in liver indicated that the regulation of the synthesis of MAP is due to a change in the rate of synthesis and/or the stability of mRNA for MAP, but not the rate of its translation.
Cole
T
T
Inglis
A
A
Nagashima
M
M
Schreiber
G
G
D000209:Acute-Phase Proteins; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001798:Blood Proteins / Q000096:biosynthesis / Q000235:genetics*; D055598:Chemical Phenomena; D002621:Chemistry; D002942:Circular Dichroism; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D007249:Inflammation / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D000511:alpha-Macroglobulins / Q000096:biosynthesis / Q000235:genetics*
D000209:Acute-Phase Proteins; D001798:Blood Proteins; D012333:RNA, Messenger; D000511:alpha-Macroglobulins; D004247:DNA