Immunoenzymatic analysis by monoclonal antibodies of bacterial lipopolysaccharides after transfer to nitrocellulose.
A rapid, sensitive immunoenzymatic technique for the analysis of lipopolysaccharide (LPS) from gram-negative bacteria using monoclonal antibodies is described. After separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the LPS was either stained with silver or electrophoretically transferred to nitrocellulose. After reaction with anti-LPS monoclonal antibodies, the transferred antigens were visualized by reaction with alkaline-phosphatase-labelled anti-mouse antibodies and a substrate containing naphthol phosphoric acid and Fast Red.
Sidberry
H
H
Kaufman
B
B
Wright
D C
DC
Sadoff
J
J
D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000942:Antigens, Bacterial / Q000032:analysis*; D003101:Collodion; D000939:Epitopes; D004926:Escherichia coli / Q000276:immunology; D007124:Immunoenzyme Techniques; D007163:Immunosorbent Techniques; D008070:Lipopolysaccharides / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000276:immunology*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000939:Epitopes; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D003101:Collodion