4-Hydroxynonenal induces a DNA-binding protein similar to the heat-shock factor.
By using a gel mobility assay, we have shown that treatment of HeLa cells with 4-hydroxynonenal, a major product of the peroxidation of membrane lipids and an inducer of heat-shock proteins, has the same effect as heat shock in causing the appearance of a protein which binds to the sequence of DNA specific for the induction of heat-shock genes. Lipoperoxidation and heat exposure seem to share a common mechanism of specific gene activation.
Cajone
F
F
Istituto di Patologia Generale dell'Universita degli Studi di Milano, Italy.
Salina
M
M
Benelli-Zazzera
A
A
D000447:Aldehydes / Q000494:pharmacology*; D001667:Binding, Competitive; D015342:DNA Probes; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D006360:Heat-Shock Proteins / Q000096:biosynthesis; D006358:Hot Temperature; D006801:Humans; D015227:Lipid Peroxidation; D011485:Protein Binding / Q000187:drug effects
D000447:Aldehydes; D015342:DNA Probes; D004268:DNA-Binding Proteins; D006360:Heat-Shock Proteins; C027576:4-hydroxy-2-nonenal