title: Characterization of the high pH wobble structure of the 2-aminopurine.cytosine mismatch by N-15 NMR spectroscopy.
abstract: Transition mutations induced by the base analogue 2-aminopurine arise via the formation of AP.C base pairs during DNA replication. We report here the results of N-15 NMR studies on a duplex oligonucleotide containing N-15 enriched AP and C residues. At high pH (8.6) the AP.C base pair is predominantly wobble. This is the first report on use of a site specifically N-15 enriched oligonucleotide as a probe of aberrant base pairing in DNA.
authors.affiliation: Division of Pediatrics, City of Hope National Medical Center, Duarte, CA.
authors.forename: L C
authors.initials: LC
authors.lastname: Sowers
authors.forename: R
authors.initials: R
authors.lastname: Eritja
authors.forename: F M
authors.initials: FM
authors.lastname: Chen
authors.forename: T
authors.initials: T
authors.lastname: Khwaja
authors.forename: B E
authors.initials: BE
authors.lastname: Kaplan
authors.forename: M F
authors.initials: MF
authors.lastname: Goodman
authors.forename: G V
authors.initials: GV
authors.lastname: Fazakerley
mesh_terms: D015075:2-Aminopurine; D000225:Adenine / Q000031:analogs & derivatives; D001482:Base Composition; D003596:Cytosine; D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009587:Nitrogen Isotopes; D009690:Nucleic Acid Conformation
chemical_list: D009587:Nitrogen Isotopes; D015075:2-Aminopurine; D003596:Cytosine; D000225:Adenine