[Colorimetric determination of isoniazid in aqueous solutions and biological fluids].
Method has been developed for determination of tuberculostatic isoniazid in aqueous solutions and biological fluids. The principle of the method is that hydrazine group of isoniazid reacts with 1-fluoro-2,4-dinitrobenzene forming a red chromophor in alkaline medium. The absorbency maximum of the chromophor is at 530 nm. Biological fluids were deproteinized and diluted with acetonitrile then adjusted to pH = 8.2 with trometamol-hydrochloric acid buffer and reacted with 1-fluoro-2,4-dinitrobenzene dissolved in acetonitrile. The chromphore was measured colorimetricaly against a blank solution which did not contain isoniazid. The method was calibrated in the range of 1-30 mg/l isoniazid and reproducibility was checked with recovery % determinations.
Csiba
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A
D001826:Body Fluids / Q000032:analysis*; D003124:Colorimetry / Q000379:methods; D006801:Humans; D007538:Isoniazid / Q000032:analysis* / Q000097:blood; D012996:Solutions
D012996:Solutions; D007538:Isoniazid
Kolorimetriás módszer izoniazid /INH/ meghatározására vizes oldatából és biológiai folyadékokból.