title: High resolution R banding in amniotic fluid cells using the BrdU-Hoechst 33258-Giemsa (RBG) technique.
abstract: While standard Giemsa banding is generally adequate for amniotic fluid chromosome analysis, small deletions, duplications, or translocation breakpoints involving G-negative bands may be difficult to appreciate. We report a method for producing high resolution R banding in human amniotic fluid cultures using the BrdU-Hoechst 33258-Giemsa (RBG) technique. RBG banding can be useful in confirming and precisely defining structural abnormalities in amniotic fluid cultures.
authors.forename: S W
authors.initials: SW
authors.lastname: Cheung
authors.forename: J P
authors.initials: JP
authors.lastname: Crane
authors.forename: A C
authors.initials: AC
authors.lastname: Burgess
mesh_terms: D000653:Amniotic Fluid / Q000166:cytology; D001399:Azure Stains; D006690:Bisbenzimidazole; D001973:Bromodeoxyuridine; D002869:Chromosome Aberrations / Q000175:diagnosis*; D002871:Chromosome Banding / Q000379:methods*; D025063:Chromosome Disorders; D005260:Female; D006801:Humans; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*
chemical_list: D001399:Azure Stains; D001973:Bromodeoxyuridine; D006690:Bisbenzimidazole