Preparation of adsorbents for affinity chromatography using TSKgel Tresyl-Toyopearl 650M.
The optimum conditions for the coupling of proteins were investigated using TSKgel Tresyl-Toyopearl 650M. They were dependent on the proteins coupled. For example, when soybean trypsin inhibitor was coupled at pH 8 the coupling was completed within 1 h and the subsequent adsorption capacity for trypsin was maximal. Longer coupling times decreased the adsorption capacity due to multi-point attachment. The adsorbents obtained were successfully used for affinity chromatography in a short time.
Nakamura
K
K
Central Research Laboratory, Tosoh Corporation, Yamaguchi, Japan.
Toyoda
K
K
Kato
Y
Y
Shimura
K
K
Kasai
K
K
D000327:Adsorption; D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods*; D003208:Concanavalin A / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000032:analysis; D008024:Ligands; D011108:Polymers; D013205:Staphylococcal Protein A / Q000378:metabolism; D013696:Temperature; D014361:Trypsin Inhibitors / Q000032:analysis
D007074:Immunoglobulin G; D008024:Ligands; D011108:Polymers; D013205:Staphylococcal Protein A; C062163:TSKgel Tresyl-Toyopearl 650M; D014361:Trypsin Inhibitors; D003208:Concanavalin A