title: Glycoprotein PAS-0 from the milk fat globule membrane carries antigenic determinants for epithelial membrane antigen.
abstract: Epithelial membrane antigen (EMA) can be found in human tissues using antisera raised against defatted human cream. PAS-0 is a glycoprotein which has been extracted from human milk fat globule membranes. Using a polyclonal antiserum and a series of monoclonal antibodies, we have shown that the major antigenic determinant for EMA is carried by PAS-0. A more detailed comparison of the two glycoproteins has been made by establishing a set of radioimmunoassays using the different antibodies.
authors.forename: M G
authors.initials: MG
authors.lastname: Ormerod
authors.forename: J
authors.initials: J
authors.lastname: McIlhinney
authors.forename: K
authors.initials: K
authors.lastname: Steele
authors.forename: M
authors.initials: M
authors.lastname: Shimizu
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000276:immunology*; D002241:Carbohydrates / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis*; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D008565:Membrane Proteins / Q000276:immunology*; D018396:Mucin-1; D011863:Radioimmunoassay
chemical_list: D000596:Amino Acids; D000941:Antigens; D002241:Carbohydrates; D000939:Epitopes; D006023:Glycoproteins; D008565:Membrane Proteins; D018396:Mucin-1