An evaluation of the Coulter VCS differential counter.
An evaluation of the Coulter VCS (volume, conductivity, light scatter) automated differential counter demonstrated satisfactory correlations with manual 800-cell differential counts for neutrophils, lymphocytes, monocytes and eosinophils. For the detection of abnormal cells, 12.2% of samples gave false-negative results on the VCS, and 13.4% gave false-positive results. However, only 0.8% of the false-negatives would be expected to be picked up by a standard 100-cell manual differential count. Carry-over accorded to manufacturer's specifications and throughput was 60 samples/h. Reference normal ranges have been established and the instrument's precision and performance with leucopenic and neonatal blood samples assessed.
Barnard
D F
DF
Department of Haematology, Middlesex Hospital Medical School, London.
Barnard
S A
SA
Carter
A B
AB
Patterson
K G
KG
Yardumian
A
A
Machin
S J
SJ
D000328:Adult; D001491:Basophils; D003201:Computers; D004804:Eosinophils; D005069:Evaluation Studies as Topic; D006402:Hematologic Diseases / Q000097:blood; D006801:Humans; D007231:Infant, Newborn; D007958:Leukocyte Count / Q000295:instrumentation*; D007970:Leukopenia / Q000097:blood; D008214:Lymphocytes; D008875:Middle Aged; D009000:Monocytes; D009504:Neutrophils; D012016:Reference Values; D013997:Time Factors