Kinetics of HDL-apo A-I in the WHHL rabbit, an animal model of familial hypercholesterolemia.
Plasma high density lipoproteins (HDL) and their major protein, apolipoprotein (apo) A-I, are deficient in homozygous Watanabe heritable hyperlipidemic (WHHL) rabbits. As the pathophysiology of subnormal HDL-apo A-I is unclear, we examined kinetic parameters of HDL-apo A-I in WHHL rabbits and normolipidemic Japanese White (control) rabbits. The total plasma mass of HDL-apo A-I was significantly smaller (P less than 0.01) in WHHL rabbits than in controls (less than one-third). Mean fractional catabolic rates (FCR) computed from the specific radioactivity decay curves of HDL-apo A-I were significantly greater (P less than 0.01) in WHHL rabbits than in controls (0.874 +/- 0.052 vs. 0.502 +/- 0.060/day, respectively). Mean synthetic rates of HDL-apo A-I were significantly lower (P less than 0.01) in WHHL rabbits than in controls (8.67 +/- 0.59 vs. 18.14 +/- 3.75 mg/kg body weight/day, respectively). We conclude that the deficiency of plasma HDL-apo A-I in the WHHL rabbits resulted from an increase in FCR and a decrease in apo A-I synthesis.
Saku
K
K
Department of Internal Medicine, School of Medicine, Fukuoka University, Japan.
Yamamoto
K
K
Sakai
T
T
Yanagida
T
T
Hidaka
K
K
Sasaki
J
J
Arakawa
K
K
D000818:Animals; D001054:Apolipoproteins A / Q000378:metabolism*; D001835:Body Weight; D004195:Disease Models, Animal; D006938:Hyperlipoproteinemia Type II / Q000378:metabolism*; D008075:Lipoproteins, HDL / Q000378:metabolism*; D011817:Rabbits
D001054:Apolipoproteins A; D008075:Lipoproteins, HDL