Ca2+-dependent inhibition by trifluoperazine of the Na+-Ca2+ carrier in mitoplasts derived from heart mitochondria.
The interaction of trifluoperazine and extramitochondrial Ca2+ with the heart mitochondrial Na+-Ca2+ carrier has been investigated. External Ca2+ inhibits the carrier equally in mitochondria and mitoplasts in which the outer membrane is lysed. Sensitivity to Ca2+ is not removed by washing mitoplasts under varied conditions. Trifluoperazine is a potent inhibitor of the carrier in mitoplasts but not in mitochondria. Trifluoperazine inhibition in mitoplasts depends markedly on the presence of extramitochondrial Ca2+ (2 microM).
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D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology*; D002352:Carrier Proteins / Q000037:antagonists & inhibitors*; D003574:Cytochrome c Group / Q000494:pharmacology; D008929:Mitochondria, Heart / Q000378:metabolism*; D051381:Rats; D012430:Ruthenium Red / Q000494:pharmacology; D019831:Sodium-Calcium Exchanger; D014268:Trifluoperazine / Q000494:pharmacology*
D002352:Carrier Proteins; D003574:Cytochrome c Group; D019831:Sodium-Calcium Exchanger; D012430:Ruthenium Red; D014268:Trifluoperazine; D002118:Calcium