title: Isolation of populations of antipeptide antibodies directed against different epitopes of the same fragment.
abstract: Rabbit antibodies against small peptides may be composed by subpopulations recognizing different epitopes made likely by few amino acids. This explains the frequent crossreactivity of antipeptide antibodies with unrelated peptides. A suitable use of immunoadsorbents is suggested to obtain truly specific antibodies able to react with restricted amino acid sequences.
authors.forename: A
authors.initials: A
authors.lastname: Chersi
authors.forename: C
authors.initials: C
authors.lastname: Greger
authors.forename: R A
authors.initials: RA
authors.lastname: Houghten
mesh_terms: D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D000918:Antibody Specificity; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis*; D007164:Immunosorbents; D010455:Peptides / Q000276:immunology*; D011817:Rabbits
chemical_list: D000906:Antibodies; D000939:Epitopes; D007164:Immunosorbents; D010455:Peptides