title: In vitro development of metacyclic Trypanosoma simiae derived from bloodstream trypomastigotes.
abstract: A stock of Trypanosoma simiae was transformed into procyclic forms at 26 C in a semi-defined maintenance medium. After transformation, the trypanosomes were maintained in a modified Eagle's MEM medium. On day 35 of cultivation, epimastigotes attached to the bottom of the culture flask. From day 44 onwards, metacyclic-like trypanosomes were observed. Subcutaneous injections into pigs of trypanosome suspensions obtained from cultures on day 10 were not infective, whereas culture-derived metacyclics (days 44, 63 and 69) were highly pathogenic.
authors.affiliation: Kenya Trypanosomiasis Research Institute, Kikuyu, Kenya.
authors.forename: E
authors.initials: E
authors.lastname: Zweygarth
authors.forename: I D
authors.initials: ID
authors.lastname: Gumm
authors.forename: M A
authors.initials: MA
authors.lastname: Gray
authors.forename: J K
authors.initials: JK
authors.lastname: Cheruiyot
authors.forename: P
authors.initials: P
authors.lastname: Webster
authors.forename: R
authors.initials: R
authors.lastname: Kaminsky
mesh_terms: D000818:Animals; D003470:Culture Media; D008854:Microscopy, Electron; D013552:Swine; D013553:Swine Diseases / Q000469:parasitology*; D014345:Trypanosoma / Q000254:growth & development* / Q000472:pathogenicity / Q000648:ultrastructure; D014353:Trypanosomiasis, African / Q000469:parasitology / Q000662:veterinary*
chemical_list: D003470:Culture Media