Quantitation of homoharringtonine in plasma by high-performance liquid chromatography with amperometric detection.
A high-performance liquid chromatographic procedure was developed for the quantitation of homoharringtonine in plasma. Harringtonine was used as an internal standard, and 1 ml of sample was required. The single-step extraction with dichloromethane resulted in almost 100% recovery for homoharringtonine and harringtonine. Analysis was performed on a reversed-phase CN column with amperometric detection. Chromatography was completed in 12 min. At an oxidation potential of +1.0 V, the detection limit was 1 ng/ml at a signal-to-noise ratio of 2. The mean analytical recovery for homoharringtonine was 99.5%. The within-run precision and between-run precision were both less than 11%. The method is equally applicable for plasma or serum, and it has been demonstrated to be applicable for study of the pharmacokinetics of homoharringtonine in patients suffering from acute non-lymphocytic leukaemia.
Chan
Y P
YP
Clinical Biochemistry Unit, University of Hong Kong, Queen Mary Hospital Compound, Pokfulam.
Lee
F W
FW
Siu
T S
TS
D000470:Alkaloids / Q000097:blood*; D000972:Antineoplastic Agents, Phytogenic / Q000097:blood* / Q000493:pharmacokinetics; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D006248:Harringtonines / Q000097:blood* / Q000493:pharmacokinetics; D000077863:Homoharringtonine; D006801:Humans; D007938:Leukemia / Q000097:blood; D010084:Oxidation-Reduction
D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D006248:Harringtonines; D000077863:Homoharringtonine