Lambda plac10 transducing bacteriophage: DNA primary structure of the region of the abnormal excision.
In studying molecular mechanisms of the formation of transducing bacteriophages, we have elucidated the primary structure of the phage-bacterial DNA junction which resulted from the abnormal excision of the lambda plac10 phage. The process is structurally similar to the excision of the lambda plac5 phage and involves, in both cases, highly homological DNA stretches approximately 20 bp long, one of them being a part of the Z-Y spacer of the lac operon and possessing a developed secondary structure. The conception of regioselective recombination as a type of illegitimate recombinational process with a certain degree of site-specificity is suggested.
Shpakovski
G V
GV
Institute of Bioorganic Chemistry, Byelorussian SSR Academy of Sciences, Minsk.
Karakashly
M P
MP
Berlin YuA
D001435:Bacteriophages / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis*; D005798:Genes, Bacterial; D007763:Lac Operon; D009875:Operator Regions, Genetic; D011994:Recombinant Proteins / Q000032:analysis; D012689:Sequence Homology, Nucleic Acid; D014161:Transduction, Genetic
D004269:DNA, Bacterial; D011994:Recombinant Proteins