Pepsinogen secretion from cultured rat gastric mucosal cells.
Rat gastric mucosal cells were isolated with the aid of 0.1% collagenase and Dispase. Pepsinogen secretion from these cells was stimulated by carbachol, cholecystokinin octapeptide (CCK(S)-8) and pentagastrin, but not by histamine. Attempts to obtain a sufficient number of cells using a higher concentration of Dispase resulted in disappearance of the responses to secretagogues. However, when gastric mucosal cells thus prepared were cultured for 24 h in a CO2 incubator, they were found to respond not only to carbachol, CCK(S)-8 and pentagastrin, but also to histamine, resulting in an increase in pepsinogen secretion. The secretagogue-induced pepsinogen secretion was inhibited by its antagonist in a dose-dependent manner. These results suggest that the receptor present in chief cells for pepsinogen secretion was destroyed during the isolation procedure and regenerated during culture.
Tani
S
S
Tanaka
T
T
Kudo
Y
Y
Takahagi
M
M
D000818:Animals; D002478:Cells, Cultured; D005753:Gastric Mucosa / Q000166:cytology / Q000378:metabolism*; D010435:Pepsinogens / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D010435:Pepsinogens