Peptides of the corticoliberin superfamily attenuate thermal and neurogenic inflammation in rat pawskin.
The paws of pentobarbital-anesthetized rats were immersed in 48 or 58 degree C water for 5 min and the thermal inflammatory response to heat was measured by Evans blue dye leakage into the pawskin. Sauvagine, sucker fish urotensin I and human/rat corticotropin-releasing factor (CRF), homologous peptides belonging to the corticoliberin superfamily, injected i.v. 10 min before heat exposure, inhibited dye leakage with ED50 (nmol/kg) of 0.44, 1.5 and 5.9, respectively. The same rank order of potency was observed when the peptides were tested against the protein extravasation evoked by antidromic stimulation of the rat saphenous nerve. The alpha-helical CRF-(-9-41) antagonist, administered alone at 24 nmol/kg i.v., did not affect the heat-induced dye leakage into skin; but it attenuated the effects of h/rCRF, urotensin I and sauvagine. h/r[Tyr0]CRF, ovine[Nle21,Tyr32]CRF and h/r[Met(O)21,38]CRF, administered 24 nmol/kg i.v., did not affect the dye leakage response to heat. Peptides of the corticoliberin superfamily may have the unusual property of making small blood vessels less permeable when responding to injurious stimuli.
Wei
E T
ET
School of Public Health, University of California, Berkeley 94720.
Kiang
J G
JG
D029845:Amphibian Proteins; D000818:Animals; D000893:Anti-Inflammatory Agents; D002199:Capillary Permeability / Q000187:drug effects; D003346:Corticotropin-Releasing Hormone / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology*; D005528:Foot; D006358:Hot Temperature; D007249:Inflammation / Q000139:chemically induced / Q000503:physiopathology*; D007279:Injections, Subcutaneous; D008297:Male; D036361:Peptide Hormones; D010455:Peptides / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012867:Skin / Q000187:drug effects / Q000473:pathology; D013256:Steroids; D014579:Urotensins / Q000494:pharmacology
D029845:Amphibian Proteins; D000893:Anti-Inflammatory Agents; D036361:Peptide Hormones; D010455:Peptides; D013256:Steroids; D014579:Urotensins; C025727:sauvagine; D003346:Corticotropin-Releasing Hormone; C029676:urotensin I