Immunocytochemistry of myelin basic proteins in adult rat oligodendroglia.
Immunocytochemical demonstration of myelin basic protein (MBP) in oligodendroglia of the developing rat and human central nervous system has been reported. However, reaction with MBP antiserum was detectable only prior to and during the early phase of myelination. The lack of reaction with still actively myelinating and mature oligodendroglia has been puzzling. We report here that by the use of mild fixation and by a modification of the staining technique previously used for detection of MBP on vibratome sections, staining of oligodendroglia in sections of adult rat brain has been achieved.
Sternberger
N H
NH
del Cerro
C
C
Kies
M W
MW
Herndon
R M
RM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001253:Astrocytes / Q000276:immunology; D006168:Guinea Pigs / Q000276:immunology; D007106:Immune Sera / Q000276:immunology; D008297:Male; D004676:Myelin Basic Protein / Q000276:immunology* / Q000502:physiology; D009457:Neuroglia / Q000276:immunology*; D009836:Oligodendroglia / Q000276:immunology* / Q000502:physiology; D011817:Rabbits / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D012756:Sheep / Q000276:immunology
D000911:Antibodies, Monoclonal; D007106:Immune Sera; D004676:Myelin Basic Protein