[Viability assay for liver transplantation].
Various parameters were investigated to know whether a viability assay before liver transplantation was possible. Two series of experiment were performed. The first consisted of 2 groups of ischemically injured canine livers which had definitely different viability. GPT, bilirubin, lactate pyruvate ratio, ketone body ratio ammonium in the perfusate, hepatic tissue flow, and hepatic tissue oxygen consumption were measured during hypothermic machine perfusion at 6 degree C. As a result, tissue flow and tissue oxygen consumption were found to be good parameters because they indicated the viabilities of the isolated liver grafts accurately and instantly in the both groups. In the second series, 9 ischemically injured canine livers were hypothermically perfused to assay their viability and orthotopically transplanted. In the cases that the oxygen consumption was below 3.0 mumol/min./100g, no grafts sustained the lives of the recipients. We concluded that if we measure the oxygen consumption of hypothermically perfused livers, we can eliminate the low viability livers before transplantation and avoid primary graft non-function.
Uematsu
T
T
Second Department of Surgery, Chiba University School of Medicine, Japan.
D000818:Animals; D004285:Dogs; D006085:Graft Survival; D007036:Hypothermia, Induced; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism / Q000473:pathology*; D008102:Liver Circulation; D016031:Liver Transplantation; D010101:Oxygen Consumption; D010477:Perfusion; D014022:Tissue Survival