title: Maximal activities of key enzymes of glutaminolysis, glycolysis, Krebs cycle and pentose-phosphate pathway of several tissues in mature and aged rats.
abstract: 1. The maximum activities of some key enzymes, which provide a quantitative indices of flux through several important pathways have been measured in brain, liver, muscle, white and brown adipose tissue and lymphocytes of mature and aged rats. 2. The results were expressed as mumol/min per g fresh weight and nmol/min per mg protein. 3. On the both basis, as compared to mature rats, hexokinase activity is decreased in brown adipose tissue and increased in soleus muscle. 4. Glucose-6-phosphate dehydrogenase activity is decreased in most tissues and increased in brain. 5. Citrate synthase activity, which provides a qualitative index of the Krebs cycle, is decreased in white adipose tissues and lymphocytes. 6. Glutaminase activity is decreased in brain, white and brown adipose tissues but is increased in lymphocytes.
authors.affiliation: Department of Physiology and Biophysics, USP, Brasil.
authors.forename: A F
authors.initials: AF
authors.lastname: de Almeida
authors.forename: R
authors.initials: R
authors.lastname: Curi
authors.forename: P
authors.initials: P
authors.lastname: Newsholme
authors.forename: E A
authors.initials: EA
authors.lastname: Newsholme
mesh_terms: D000273:Adipose Tissue / Q000201:enzymology; D000375:Aging / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000201:enzymology; D002950:Citrate (si)-Synthase / Q000378:metabolism*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D005972:Glutaminase / Q000378:metabolism*; D006593:Hexokinase / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D008198:Lymph Nodes / Q000201:enzymology; D008297:Male; D009132:Muscles / Q000201:enzymology; D009928:Organ Specificity; D007652:Oxo-Acid-Lyases / Q000378:metabolism*; D051381:Rats
chemical_list: D005954:Glucosephosphate Dehydrogenase; D002950:Citrate (si)-Synthase; D006593:Hexokinase; D005972:Glutaminase; D007652:Oxo-Acid-Lyases