Evaluation of human and simian immunodeficiency virus plaque and neutralization assays.
A number of CD4+ T cell lines were compared for their ability to act as target cells for human immunodeficiency virus (HIV) infection in syncytium- and plaque-forming assays. MT-4 and C8166 cells were the most sensitive indicator cells for HIV- and simian immunodeficiency virus (SIV)-induced cytopathic effects, and gave rise to macroscopic (MT-4) and microscopic (C8166) plaques. The MT-4 plaque assay was evaluated for the measurement of HIV- and SIV-neutralizing antibodies.
McKeating
J A
JA
Institute of Cancer Research, Chester Beatty Laboratories, London, U.K.
McKnight
A
A
McIntosh
K
K
Clapham
P R
PR
Mulder
C
C
Weiss
R A
RA
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000382:microbiology*; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D006678:HIV / Q000254:growth & development / Q000276:immunology / Q000502:physiology*; D015483:HIV Antibodies / Q000032:analysis; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D009500:Neutralization Tests; D015302:Simian Immunodeficiency Virus / Q000254:growth & development / Q000276:immunology / Q000502:physiology*; D010948:Viral Plaque Assay
D000914:Antibodies, Viral; D015483:HIV Antibodies; D007106:Immune Sera