title: Secretory character of a group of isoproterenol-induced polypeptides in mouse parotid glands.
abstract: The secretory nature of the isoproterenol-induced mouse parotid polypeptides C, D, E, F, and G (molecular weights 64,000, 61,000, 51,500, 38,000, and 37,000, respectively) is documented. Polypeptides C, D, E, F, and G, accumulated in response to successive daily stimulations with isoproterenol, were detected in a fraction enriched in hypertrophic parotid acinar cells. These cells, characterized by an increased content of cytoplasmic granules, maintain a secretory responsiveness to isoproterenol, which has been evidenced by light microscopy, enzymatic analysis, and unidimensional SDS-polyacrylamide gel electrophoresis. Thus, a parallelism in the loss and recovery of both secretory granules, alpha-amylase and polypeptides C, D, E, F, and G, was observed. Moreover, after secretion stimulation, polypeptides C, D, E, F, and G were detected in the fluid collected directly from parotid gland cannulation. Given the secretory character of polypeptides C, D, E, F, and G, mechanisms explaining both their progressive accumulation along the chronic administration of isoproterenol, as well as their progressive disappearance observed after suspending that treatment, are discussed.
authors.affiliation: Department of Cell Biology and Genetics, Faculty of Medicine, University of Chile, Santiago.
authors.forename: R O
authors.initials: RO
authors.lastname: López Solís
authors.forename: D
authors.initials: D
authors.lastname: Miranda
authors.forename: C
authors.initials: C
authors.lastname: Alliende
authors.forename: J
authors.initials: J
authors.lastname: González
authors.forename: N
authors.initials: N
authors.lastname: Díaz
authors.forename: H
authors.initials: H
authors.lastname: Díaz
authors.forename: F
authors.initials: F
authors.lastname: Ruiz
mesh_terms: D000818:Animals; D007545:Isoproterenol / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010306:Parotid Gland / Q000187:drug effects / Q000378:metabolism*; D010455:Peptides / Q000032:analysis / Q000378:metabolism*; D012471:Salivary Proteins and Peptides / Q000032:analysis / Q000378:metabolism*; D000516:alpha-Amylases / Q000378:metabolism
chemical_list: D010455:Peptides; D012471:Salivary Proteins and Peptides; D000516:alpha-Amylases; D007545:Isoproterenol