A procedure for urease and protein extraction from staphylococci.
Staphylococcal cell protein and urease can be solubilized after growth in Todd-Hewitt broth supplemented with 0.5% yeast extract by extraction for 18-24 h in phosphate buffer, pH 7.0. In general 20% (but up to 100%) of the urease present in the original cells could be solubilized. Less protein was solubilized. Species examined included coagulase-negative staphylococci, Staphylococcus intermedius and Staph. aureus. Extracts of Staph. epidermidis prepared by this procedure gave electrophoretic urease and protein patterns similar to those prepared by sonication. The procedure was simple and minimized handling of the cells.
Sissons
C H
CH
Dental Research Unit, Medical Research Council of New Zealand, Wellington.
Hancock
E M
EM
Perinpanayagam
H E
HE
Cutress
T W
TW
D001426:Bacterial Proteins / Q000032:analysis / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D013210:Staphylococcus / Q000032:analysis / Q000201:enzymology*; D014510:Urease / Q000032:analysis / Q000302:isolation & purification*
D001426:Bacterial Proteins; D014510:Urease