Investigation of the allergenic activity of isolated fractions of a standardized partly purified whole mite body (Dermatophagoides pteronyssinus) extract.
Twelve fractions of a molecular weight range of 1.35-670.00 kilodaltons (kD) were isolated from a biologically standardized partly purified whole mite body extract (Dermatophagoides pteronyssinus) by preparative size exclusion high-performance liquid chromatography. The allergenic activity and the antigen and allergen patterns of the isolated fractions were investigated by RAST, RAST inhibition and crossed (radio)immunoelectrophoresis (CIE/CRIE). By CRIE, each of the fractions showed allergen patterns, mostly of different compositions. Each fraction showed allergenic activity of different degrees by RAST inhibition. The highest allergenic activity could be measured by RAST inhibition with fractions which contained predominantly the major allergens Der pI and PY as detected by CRIE. Also proteins of higher molecular weights (greater than 158 kD) showed IgE-binding capacities. Nearly all antigens detected by CIE could be identified as allergens using CRIE.
Wahl
R
R
Department for Research and Development of Allergen Extract Preparations, Allergopharma, Reinbek b, Hamburg.
Meineke
D
D
Maasch
H J
HJ
Schultze-Werninghaus
G
G
D000485:Allergens / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology; D007123:Immunoelectrophoresis, Two-Dimensional; D007073:Immunoglobulin E / Q000378:metabolism; D008925:Mites / Q000276:immunology*; D008970:Molecular Weight; D011506:Proteins / Q000276:immunology; D011852:Radioallergosorbent Test
D000485:Allergens; D011506:Proteins; D007073:Immunoglobulin E