Ca2+ interaction with phospholipid bilayers studied by multifrequency phase fluorometry.
Calcium interaction with phospholipid membranes containing phosphatidic acid is studied by multifrequency phase fluorometry, using DPH as fluorescent molecule. DPH decay is analysed by a continuous distribution of lifetimes. The results suggest an increase of membrane heterogeneity at low calcium concentrations, without changes in the polarity of the environment surrounding the probe.
Zolese
G
G
Institute of Biochemistry, Medical School, Ancona, Italy.
Curatola
G
G
D002118:Calcium / Q000378:metabolism*; D004161:Diphenylhexatriene; D005470:Fluorometry; D008051:Lipid Bilayers / Q000378:metabolism*; D010712:Phosphatidic Acids / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*
D008051:Lipid Bilayers; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D010743:Phospholipids; D004161:Diphenylhexatriene; D002118:Calcium