Enantiomeric selectivity of adenosine transport systems in mouse erythrocytes and L1210 cells.
In mediating the entry of adenosine into mouse erythrocytes and mouse leukaemia L1210 cells, nucleoside transport systems were stereoselective, showing a marked preference for the D-enantiomer of adenosine (D-Ado). Inward zero-trans fluxes of the mirror-image isomer, L-adenosine (L-Ado), in those cells were slow relative to those of D-Ado. Contributing to L-Ado fluxes in both cell types were (i) a transporter-mediated process of high nitrobenzylthioinosine-sensitivity and (ii) simple diffusion.
Gati
W P
WP
Department of Pharmacology, University of Alberta, Edmonton, Canada.
Dagnino
L
L
Paterson
A R
AR
D000241:Adenosine / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D004910:Erythrocyte Membrane / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D007939:Leukemia L1210 / Q000378:metabolism*; D051379:Mice; D012964:Sodium / Q000378:metabolism; D013237:Stereoisomerism; D013868:Thioinosine / Q000031:analogs & derivatives / Q000494:pharmacology
D013868:Thioinosine; D012964:Sodium; C001789:4-nitrobenzylthioinosine; D000241:Adenosine