Internal entry of ribosomes and ribosomal scanning involved in hepatitis B virus P gene expression.
The recent demonstration that the synthesis of duck hepatitis B virus (HBV) reverse transcriptase does not require translational frameshifting and the finding that poliovirus mRNA translation occurs in a cap-independent manner by internal binding of ribosomes in the 5' noncoding region led us to design experiments to test the hypothesis of internal entry of ribosomes on C gene mRNA for HBV P gene expression. We show that in human cells, translation can be initiated at the first AUG of the HBV P gene by entry of ribosomes in a region located upstream of the P gene. Moreover, the leaky scanning of ribosomes observed on the first AUG of the HBV P gene could be responsible for the synthesis of the two forms of reverse transcriptase described for HBV particles.
Jean-Jean
O
O
Laboratoire de Biologie Moléculaire de la Réplication, Centre National de la Recherche Scientifique ER-272, Villejuif, France.
Weimer
T
T
de Recondo
A M
AM
Will
H
H
Rossignol
J M
JM
D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D004274:DNA, Recombinant / Q000378:metabolism; D015870:Gene Expression; D005814:Genes, Viral; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D008979:Moloney murine leukemia virus / Q000235:genetics; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D012270:Ribosomes / Q000378:metabolism*; D014158:Transcription, Genetic; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics; D015678:Viral Structural Proteins / Q000235:genetics*
D004274:DNA, Recombinant; D012333:RNA, Messenger; D014764:Viral Proteins; D015678:Viral Structural Proteins