Decreased levels of TMV coat protein in transgenic tobacco plants at elevated temperatures reduce resistance to TMV infection.
Transgenic tobacco plants that accumulate tobacco mosaic virus (TMV) coat protein (CP) are resistant to TMV infection under standard growth conditions. The amount of CP accumulated and the degree of resistance to TMV were found to be temperature dependent. Exposure to continuous high temperatures (30-35 degrees) results in a sharp decrease in the amount of CP within 6 hr with no further change for at least 6 days. Under these conditions the transgenic plants developed typical systemic disease symptoms when inoculated with TMV although disease development was delayed. Transgenic plants which were moved from 35 to 22 degrees accumulated the normal level of CP within several hours. Transgenic tobacco plants inoculated and held at 35/25 degrees day/night cycles retained resistance to TMV infection. The level of CP mRNA was constant at each temperature and was associated with polyribosomes. On the basis of these results we suggest that the low level of CP under elevated temperature is due to instability of the TMV CP. In contrast, TMV CP levels in transgenic tomato plants also dropped under elevated temperatures yet retained high resistance to TMV.
Nejidat
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Department of Biology, Washington University, St. Louis, Missouri 63130.
Beachy
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D015152:Blotting, Northern; D002213:Capsid / Q000235:genetics* / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D009693:Nucleic Acid Hybridization; D010935:Plant Diseases; D010947:Plants, Toxic; D012333:RNA, Messenger / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis; D013696:Temperature; D014026:Nicotiana / Q000382:microbiology*; D014027:Tobacco Mosaic Virus / Q000235:genetics* / Q000378:metabolism
D012333:RNA, Messenger; D012367:RNA, Viral