title: INCA: software for consort 30 analysis of flow cytometric calcium determinations.
abstract: The INCA program converts Consort 30-generated fluorescence list mode data collected from Indo-1-stained cells to absolute intracellular calcium concentrations (nM Ca2+i). The calcium data are plotted vs. time, allowing the user to analyze the fractions of cells responding to a given stimulus. Converted files can be restored to disk after replacing FL1 and FL2 with time and calcium, respectively, for future analysis.
authors.affiliation: Department of Clinical Immunology, University of Groningen, The Netherlands.
authors.forename: J F
authors.initials: JF
authors.lastname: Keij
authors.forename: A W
authors.initials: AW
authors.lastname: Griffioen
authors.forename: T H
authors.initials: TH
authors.lastname: The
authors.forename: G T
authors.initials: GT
authors.lastname: Rijkers
mesh_terms: D001402:B-Lymphocytes / Q000032:analysis; D002118:Calcium / Q000032:analysis*; D001330:Electronic Data Processing; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D012984:Software; D013601:T-Lymphocytes / Q000032:analysis
chemical_list: D002118:Calcium