Chemical and enzymatic biotin-labeling of oligodeoxyribonucleotides.
Biotin has been converted to 2-(biotinylamido)ethanol and condensed to phosphorylated oligonucleotides in a solid phase synthesis. The 5'-biotinylated oligonucleotides were enzymatically coupled to other DNA fragments by T4 DNA ligase or T4 RNA ligase. The hybridization properties of such biotin-labeled oligonucleotide probes were studied.
Kempe
T
T
Sundquist
W I
WI
Chow
F
F
Hu
S L
SL
D001710:Biotin / Q000031:analogs & derivatives / Q000138:chemical synthesis; D055598:Chemical Phenomena; D002621:Chemistry; D003101:Collodion; D004247:DNA / Q000378:metabolism; D011088:DNA Ligases; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000235:genetics / Q000302:isolation & purification; D009841:Oligonucleotides / Q000235:genetics / Q000302:isolation & purification; D012313:RNA / Q000378:metabolism; D011130:RNA Ligase (ATP)
D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D012313:RNA; D001710:Biotin; D003101:Collodion; D004247:DNA; C045624:2-(biotinylamido)ethanol; D011088:DNA Ligases; D011130:RNA Ligase (ATP)