title: Phagemid VPCS vectors for priming, cloning and sequencing.
abstract: A phagemid was adapted for use as the vector in the vector-primer-cloner-sequencer cloning system. The use of this new vector markedly expanded the utility of this technology for the construction of cDNA libraries. Technological advantages and new capabilities include: (1) a greater number of unique restriction sites within the polylinker region; (2) the ability to produce single-stranded templates for nucleotide sequencing, and (3) a convenient means to synthesize strand-specific hybridization probes. With the use of this cloning system, a rat liver cDNA library (8.56 x 10(5) recombinants from 1 microgram of poly(A)+ RNA) was rapidly (in two days) constructed.
authors.affiliation: Department of Medical Biochemistry, Faculty of Medicine, University of Calgary, Alberta, Canada.
authors.forename: S A
authors.initials: SA
authors.lastname: Krawetz
authors.forename: D
authors.initials: D
authors.lastname: Sellos
authors.forename: N C
authors.initials: NC
authors.lastname: Wong
authors.forename: G H
authors.initials: GH
authors.lastname: Dixon
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D004274:DNA, Recombinant; D015723:Gene Library; D005822:Genetic Vectors; D010957:Plasmids; D015183:Restriction Mapping
chemical_list: D004274:DNA, Recombinant