Hybrids from equine LH: alpha enhances, beta diminishes activity.
LH hybrids were prepared by combining eLH alpha and eLH beta with the corresponding subunits of oLH, pLH and hCG. Recombinants were isolated by gel filtration and assessed by SDS-polyacrylamide gel electrophoresis under both dissociating and non-dissociating conditions. All combinations of subunits produced hybrid LH molecules. Hybrids prepared by combining eLH beta with oLH alpha, pLH alpha or hCG alpha were very inactive in rat radioligand and Leydig cell in vitro bioassays. Hybrids prepared with eLH alpha were very active in both assays. The greatest potentiating activity was observed when eLH alpha was combined with pLH beta. The resulting hybrid was 49 times as active as pLH in stimulating steroidogenesis by Leydig cells.
Bousfield
G R
GR
Liu
W K
WK
Ward
D N
DN
D000818:Animals; D001681:Biological Assay; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D018997:Chorionic Gonadotropin, beta Subunit, Human; D015292:Glycoprotein Hormones, alpha Subunit; D006736:Horses / Q000378:metabolism*; D007985:Leydig Cells / Q000187:drug effects; D007986:Luteinizing Hormone / Q000494:pharmacology*; D008297:Male; D010446:Peptide Fragments / Q000494:pharmacology*; D010908:Pituitary Hormones, Anterior / Q000494:pharmacology*; D055503:Protein Multimerization; D051381:Rats; D012756:Sheep / Q000378:metabolism; D013045:Species Specificity; D013256:Steroids / Q000096:biosynthesis; D013552:Swine / Q000378:metabolism
D006063:Chorionic Gonadotropin; D018997:Chorionic Gonadotropin, beta Subunit, Human; D015292:Glycoprotein Hormones, alpha Subunit; D010446:Peptide Fragments; D010908:Pituitary Hormones, Anterior; D013256:Steroids; D007986:Luteinizing Hormone