dNTP imbalance and DNA double strand breaks in mouse FM3A cells and the mechanism of cell death.
The mechanism of intracellular deoxyribonucleoside-triphosphate (dNTP) imbalance death of mouse mammary tumor FM3A cells was studied. When the cells were exposed to 5-fluorodeoxyuridine, deoxyadenosine, or 2-chlorodeoxyadenosine, dNTP pool imbalance resulted. The imbalance was followed by DNA double-strand breaks and subsequent cell death. The DNA double strand breaks were directly examined by means of orthogonal-field-alternation gel electrophoresis (OFAGE). Fragmented DNA band appeared to be approximately 100-200 kbp in size. The bases of 5'-termini in the DNA were cytosine and thymine. The imbalance induced endonuclease has been isolated by DEAE-agarose column chromatography.
Wataya
Y
Y
Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Watanabe
K
K
Yoshida
S
S
Hiramoto-Yoshioka
A
A
D015762:2-Chloroadenosine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D002470:Cell Survival / Q000235:genetics*; D017338:Cladribine; D004249:DNA Damage; D004273:DNA, Neoplasm / Q000378:metabolism; D003839:Deoxyadenosines / Q000494:pharmacology; D003854:Deoxyribonucleotides / Q000378:metabolism*; D005467:Floxuridine / Q000494:pharmacology; D051379:Mice; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D003839:Deoxyadenosines; D003854:Deoxyribonucleotides; D005467:Floxuridine; D015762:2-Chloroadenosine; D017338:Cladribine