Analysis of pirprofen in cerebrospinal fluid, plasma, and synovial fluid by high-performance liquid chromatography with electrochemical detection.
We describe a high-performance liquid chromatographic method, using electrochemical detection, for the determination of pirprofen in cerebrospinal fluid (CSF), plasma, and synovial fluid (SF). A C-18 column with a mobile phase containing acetonitrile acetate:phosphate buffer (pH 3.0) was employed. Samples were added with phosphoric acid, then extracted into dichloromethane, evaporated, and injected into the chromatograph. A detection potential of +0.85 V was applied on the basis of current-potential curves. Good linearity was found for each fluid in the expected range of therapeutic concentrations. The detection limit was 0.1 ng/mL for CSF, and 1 ng/mL for plasma and SF, with a recovery greater than 96% and intraday coefficient of variation less than 5% in all cases. The main advantages of this method include high specificity and sensitivity which allow the analysis of CSF and the use of small volumes of plasma and SF. The application of the method for the analysis of plasma and SF samples and the kinetic profile in CSF are shown.
Zecca
L
L
CNR, Istituto di Tecnologie Biomediche Avanzate, Milano, Italy.
Ferrario
P
P
Pirola
R
R
Zambotti
F
F
Zonta
N
N
Fraschini
F
F
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D006801:Humans; D010666:Phenylpropionates / Q000097:blood / Q000134:cerebrospinal fluid; D013582:Synovial Fluid / Q000032:analysis*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D010666:Phenylpropionates; C008924:pirprofen