title: Change in surface charge of sarcoplasmic reticulum membrane may elicit conformational change in sulfhydryl groups of membrane proteins to release calcium.
abstract: A lipophilic anion, tetraphenylboron (TPB-)-induced Ca2+ release from fragmented sarcoplasmic reticulum (SR) of frog skeletal muscle was monitored by chlortetracycline fluorescence. TPB- caused change in surface charge of the membrane and in the protein conformation with a time course similar to that of the Ca2+ release. Tetraphenylarsonium (TPA+) inhibited these effects of TPB-. Change in surface charge of SR is suggested to cause conformational change in SR membrane proteins, and then result in Ca2+ release from the SR.
authors.affiliation: Department of Physiology, Nagoya City University Medical School, Japan.
authors.forename: G H
authors.initials: GH
authors.lastname: Liu
authors.forename: T
authors.initials: T
authors.lastname: Oba
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004594:Electrophysiology; D008565:Membrane Proteins / Q000378:metabolism*; D009132:Muscles / Q000166:cytology; D011487:Protein Conformation / Q000187:drug effects; D011892:Rana catesbeiana; D012519:Sarcoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D013775:Tetraphenylborate / Q000494:pharmacology; D013997:Time Factors
chemical_list: D008565:Membrane Proteins; D013438:Sulfhydryl Compounds; D013775:Tetraphenylborate; D002118:Calcium