RFLP analysis of HLA-DR beta and -DQ beta genes in the Korean patients with insulin-dependent diabetes mellitus.
Human genomic DNA samples from 19 Korean patients and 31 controls of known serological DR antigen specificity were studied for insulin-dependent diabetes mellitus (IDDM)-associated variation in HLA-DR beta and -DQ beta restriction fragment length polymorphisms (RFLPs). Genotyping allowed for accurate assignment of HLA-DR types. For HLA-DRw6, a 12kb/DR beta/Taq I fragment was decreased in Korean IDDM (p less than 0.05). However, we could not find an increased frequency of a 12kb/DQ beta/Bam HI fragment or decreased frequency of a 3.7kb/DQ beta/Bam HI fragment in Korean IDDM. These results suggest a possible protective role of the HLA-DRw6 specificity in IDDM, irrespective of ethnic background, the absence of a specific DQ beta RFLP pattern associated with IDDM in Koreans, and the difference of the Korean population in the genetic of IDDM, compared to the Caucasoid population.
Lee
H K
HK
Rhee
B D
BD
Koh
C S
CS
Min
H K
HK
Seo
J S
JS
Kim
G R
GR
Han
H
H
Park
S W
SW
Choi
D S
DS
Yoo
H J
HJ
D044466:Asian People / Q000235:genetics; D015343:DNA Probes, HLA; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics*; D005260:Female; D005838:Genotype; D006683:HLA-DQ Antigens / Q000235:genetics*; D006684:HLA-DR Antigens / Q000235:genetics*; D006801:Humans; D007723:Korea; D008297:Male; D012150:Polymorphism, Restriction Fragment Length; D012703:Serotyping; D044465:White People / Q000235:genetics
D015343:DNA Probes, HLA; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens