A modified, prefixed, Golgi-rapid technique for the cephalopod retina.
A quick and simple method for Golgi staining of neurons and glia in the cephalopod retina is described. The main difference between this method and other Golgi-rapid protocols is that tissue is placed in sachets constructed from dialysis membrane prior to chromation and impregnation. This greatly reduces the amount of surface deposition of silver chromate and can reduce the number of elements impregnated. Together these effects produce improved Golgi staining of the cephalopod retina. Given some modifications to the osmolarity of the solutions employed, the method should be applicable to other, non-cephalopod tissues where superficial structures are obscured by surface precipitation, or neurons are arranged in thin sheets of tissue. The method employs prefixation of tissue in a fixative suitable for light or electron microscopy.
Patterson
J A
JA
D000818:Animals; D006652:Histological Techniques; D008974:Mollusca / Q000033:anatomy & histology*; D009474:Neurons / Q000648:ultrastructure; D049831:Octopodiformes; D012160:Retina / Q000033:anatomy & histology*; D013194:Staining and Labeling; D013569:Synapses / Q000648:ultrastructure