title: Japanese encephalitis virus infection in mosquitoes reared from field-collected immatures and in wild-caught males.
abstract: Immature stages of mosquitoes collected in JE endemic areas of Karnataka, India between 1985 and 1987 were reared to adults and processed for the detection and isolation of Japanese encephalitis (JE) virus in an attempt to find naturally occurring vertical transmission of the virus. Males collected during 1985-1986 were also processed. A total of 15,785 adults reared from immatures and divided into 445 pools and 1,756 wild-collected males divided into 128 pools were processed using mosquito inoculation and immunofluorescence techniques. JE virus antigen was detected in 9 pools, 4 of which yielded JE virus. These were 2 pools of males and 1 pool of female Culex tritaeniorhynchus and 1 pool of male C. pseudovishnui, suggesting vertical transmission of JE virus in the mosquitoes.
authors.affiliation: National Institute of Virology, Pune, India.
authors.forename: V
authors.initials: V
authors.lastname: Dhanda
authors.forename: D T
authors.initials: DT
authors.lastname: Mourya
authors.forename: A C
authors.initials: AC
authors.lastname: Mishra
authors.forename: M A
authors.initials: MA
authors.lastname: Ilkal
authors.forename: U
authors.initials: U
authors.lastname: Pant
authors.forename: P G
authors.initials: PG
authors.lastname: Jacob
authors.forename: H R
authors.initials: HR
authors.lastname: Bhat
mesh_terms: D000818:Animals; D003465:Culex / Q000382:microbiology; D009033:Culicidae / Q000382:microbiology*; D004664:Encephalitis Virus, Japanese / Q000302:isolation & purification*; D005260:Female; D007194:India; D007303:Insect Vectors / Q000382:microbiology*; D007814:Larva / Q000382:microbiology; D008297:Male; D011679:Pupa / Q000382:microbiology