Immunocytochemistry in the diagnosis of Whipple's disease.
In six cases of Whipple's disease the characteristic macrophages could be stained immunocytochemically using a first layer antiserum to group B streptococci. Other types of periodic acid Schiff positive macrophages were negative. The staining appears to be due to a rhamnose containing polysaccharide as shown by a blocking test. The method may be useful for confirming the diagnosis in rectal biopsy and peripheral nodes, especially if small intestinal findings are atypical.
Evans
D J
DJ
Ali
M H
MH
D005455:Fluorescent Antibody Technique; D006801:Humans; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D007421:Intestine, Small / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D013292:Streptococcus agalactiae / Q000276:immunology; D008061:Whipple Disease / Q000276:immunology*
D007106:Immune Sera