title: Expression of envelope glycoprotein (E) of Japanese encephalitis virus by recombinant vaccinia virus.
abstract: Vaccinia virus recombinants inserted with cDNA clones of Japanese encephalitis (JE) virus envelope glycoprotein (E) gene were constructed. The E gene product was detected in the recombinant virus-infected BHK21 cells by immunofluorescence (IF) and Western blotting. The intensity of IF observed was higher by the recombinant of the TK promoter--P7.5 promoter--inserted cDNA construct than by the P7.5 promoter--TK promoter--inserted cDNA construct. The E gene product was hardly detected by the recombinant carrying the TK promoter only upstream to the inserted cDNA, although the glycoprotein E mRNA had been transcribed.
authors.affiliation: Department of Virology, Nagasaki University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Haishi
authors.forename: H
authors.initials: H
authors.lastname: Imai
authors.forename: K
authors.initials: K
authors.lastname: Hirai
authors.forename: A
authors.initials: A
authors.lastname: Igarashi
authors.forename: S
authors.initials: S
authors.lastname: Kato
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000235:genetics; D004664:Encephalitis Virus, Japanese / Q000235:genetics*; D015870:Gene Expression; D006023:Glycoproteins / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D014616:Vaccinia virus / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D004274:DNA, Recombinant; D004279:DNA, Viral; D006023:Glycoproteins; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins