A, B and H antigens in normal urothelium: an immunohistochemical study using monoclonal antibodies with the avidin-biotin complex technique.
Immunohistochemical staining for the A, B and H blood group antigens was studied in 61 normal human ureters using monoclonal antibodies with avidin-biotin complex application. Thirty-seven of these were archival material, and 24 were processed prospectively. In 100 per cent of the prospectively processed ureters, A, B and H antigens were demonstrated corresponding to the blood type of the source. Archival material stained for A, B and H 65 per cent, 50 per cent and 100 per cent of the time, respectively. Serial sampling of prospectively processed ureters showed diminution of staining with prolongation of immersion in formalin. A characteristic staining pattern was found in ureters from patients with type B blood.
Seal
G M
GM
Rowland
R G
RG
Thomalla
J V
JV
Rudolph
R A
RA
Pfaff
D S
DS
Kamer
M
M
Eble
J N
JN
D000017:ABO Blood-Group System / Q000276:immunology*; D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001360:Avidin; D001710:Biotin; D002648:Child; D006651:Histocytochemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D007519:Isoantigens / Q000032:analysis*; D013194:Staining and Labeling; D014513:Ureter / Q000276:immunology*
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D007519:Isoantigens; D001360:Avidin; D001710:Biotin