[Biochemical studies of potential-dependent sodium channels].
The data on interaction of sodium channels of excited membranes with various neurotoxin groups are considered in the paper including the data on using biologically active neurotoxin derivatives as a specific label. These derivatives carry fluorescent or photoactive groups possessing high specific radioactivity, which permits developing the methods for sodium channel solubilization and solubilized preparation stabilization. The high-purified preparations are characterized by the methods of gel chromatography, centrifugation within the gradient density, gel electrophoresis and affinity chromatography. Experiments on reconstruction of solubilized preparations in the bilayer of lipid vesicles are under discussion.
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D029845:Amphibian Proteins; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D066298:In Vitro Techniques; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D008081:Liposomes / Q000378:metabolism; D008564:Membrane Potentials; D008566:Membranes / Q000378:metabolism; D009498:Neurotoxins / Q000378:metabolism / Q000494:pharmacology*; D010455:Peptides / Q000378:metabolism / Q000494:pharmacology; D012530:Saxitoxin / Q000378:metabolism / Q000494:pharmacology; D012964:Sodium / Q000378:metabolism*; D015222:Sodium Channels; D012995:Solubility; D013779:Tetrodotoxin / Q000378:metabolism / Q000494:pharmacology
D029845:Amphibian Proteins; D002352:Carrier Proteins; D007473:Ion Channels; D008081:Liposomes; D009498:Neurotoxins; D010455:Peptides; D015222:Sodium Channels; C027525:saxitoxin-binding protein, Rana catesbeiana; C023790:tetrodotoxin-binding protein; D012530:Saxitoxin; D013779:Tetrodotoxin; D012964:Sodium
Biokhimicheskie issledovaniia potentsialzavisimykh natrievykh kanalov.