Antigen-specific augmentation of murine immediate hypersensitivity-like footpad reaction by a T-cell factor in the culture supernatant of immune spleen cells.
An antigen-specific factor capable of augmenting delayed-type hypersensitivity (DH) in the culture supernatants from immune spleen cells and erythrocyte antigen has been found. These culture supernatants also augmented an immediate hypersensitivity-like reaction which appeared in advance of the classical DH reaction. In this paper, the basic characteristics and cells producing the augmentation factor (IAF) involved in immediate hypersensitivity-like reaction were investigated. Maximum activity of IAF was detected in a supernatant from 24-hr culture of immune spleen cells and antigen. In vitro antigen stimulation was essentially required for the production or release of IAF. IAF showed antigen-specificity. IAF was produced or released by T cells. In addition to these facts, the DH-augmentation factor proved to be a T-cell product.
Yamada
A
A
Himeno
K
K
Kumazawa
Y
Y
Nomoto
K
K
D000818:Animals; D000941:Antigens / Q000276:immunology; D002478:Cells, Cultured; D000939:Epitopes; D005260:Female; D006967:Hypersensitivity / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D013154:Spleen / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D000941:Antigens; D000939:Epitopes