The effect of sulphasalazine on the in vitro activation of human peripheral blood mononuclear cells by phytohemagglutinin P.
The in vitro action of sulphasalazine, BW 755-C and indomethacin on phytohemagglutinin P-induced human peripheral blood mononuclear cell activation was studied. Sulphasalazine increased, while indomethacin and BW 755-C decreased, prostaglandin E2 (PGE2) accumulation in activated cultures. When used together with indomethacin or BW 755-C, sulphasalazine did not counteract the inhibition of PGE2 caused by the other two. Sulphasalazine inhibited phytohemagglutinin P-induced cell activation in a concentration-dependent manner even when used together with indomethacin or BW 755-C. BW 755-C inhibited cell activation at 350 microM, whereas at 11 microM it only increased sulphasalazine-induced inhibition. The implications of these findings on the etiopathology of inflammatory bowel disease are discussed.
Pacheco
S
S
Departamento de Biologia Geral, Universidade Federal de Viçosa, MG, Brasil.
Jones
D B
DB
Hillier
K
K
D015772:4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine / Q000494:pharmacology*; D015232:Dinoprostone / Q000096:biosynthesis; D006801:Humans; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology*; D015212:Inflammatory Bowel Diseases / Q000209:etiology; D007963:Leukocytes, Mononuclear / Q000187:drug effects*; D010835:Phytohemagglutinins / Q000494:pharmacology*; D012460:Sulfasalazine / Q000494:pharmacology*
D010835:Phytohemagglutinins; C028784:phytohemagglutinin-P; D012460:Sulfasalazine; D015772:4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine; D015232:Dinoprostone; D007213:Indomethacin