Accessory cells in immune suppression. II. Evidence for presentation of idiotype-specific suppressor factors to B cell targets by I-A+ accessory cells.
The functional suppression of two BALB/c myelomas, MOPC315 and MOPC460, by idiotype-specific suppressor T cells is mediated by secreted factors (TsF). Idiotype-specific TsF only functions in the presence of accessory cells (AC) that can be low density splenocytes or cells of BALB/c B lymphoma A20-2J. Such AC are I-A+ but do not have to be histocompatible with the myeloma targets or the source of TsF. A20-2J cells incubated with soluble idiotype and TsF also are capable of suppressing the relevant myeloma target. The effect of TsF-pulsed A20-2J cells is idiotype specific, and these cells do not secrete immunosuppressive mediators. Finally, TsF-pulsed A20-2J cells suppress myeloma targets when the two are cultured on the same side of a cell-impermeable membrane but not when they are separated by the membrane. These results indicate that I-A+ cells are capable of concentrating and/or presenting TsF to suppressible targets, and they demonstrate a novel role for Ia-bearing cells in immune regulation.
Moser
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Kauffman
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Abbas
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D000818:Animals; D000938:Antigen-Presenting Cells / Q000145:classification / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D000939:Epitopes; D005260:Female; D006183:H-2 Antigens; D000949:Histocompatibility Antigens Class II; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D008222:Lymphokines / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010641:Phenotype; D010954:Plasmacytoma / Q000276:immunology; D013154:Spleen / Q000166:cytology; D013491:Suppressor Factors, Immunologic
D000939:Epitopes; D006183:H-2 Antigens; D000949:Histocompatibility Antigens Class II; D007130:Immunoglobulin Idiotypes; D008222:Lymphokines; D013491:Suppressor Factors, Immunologic