Serum-induced stimulation of snRNA synthesis in mouse 3T3 fibroblasts.
Small nuclear RNAs (snRNAs) from quiescent and serum-stimulated 3T3 cultures, labeled with [3H]uridine [( 3H]U), were electrophoresed in polyacrylamide-urea slab gels and revealed by staining with ethidium bromide and by fluorography. Judged by labeling with [3H]U, synthesis of 7S and U1-U6 RNAs was very low or absent in quiescent cultures. The serum-induced transition of 3T3 cells from a resting to a growing state was accompanied by an early, apparently sequential stimulation of snRNA synthesis; stimulated synthesis of 7S, U1, U2, U3, U4 and U6 RNAs coincided in time with serum-induced stimulation of 45S pre-ribosomal RNA (pre-rRNA) and heterogeneous nuclear RNA (hnRNA) synthesis.
Tiercy
J M
JM
Weil
R
R
D000818:Animals; D001769:Blood; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004996:Ethidium; D005347:Fibroblasts / Q000378:metabolism*; D005470:Fluorometry; D051379:Mice; D012313:RNA / Q000096:biosynthesis*; D012342:RNA, Small Nuclear; D014529:Uridine / Q000378:metabolism
D012342:RNA, Small Nuclear; D012313:RNA; D004996:Ethidium; D014529:Uridine