[Preparation and properties of prostaglandin D2 labelled with tritium with high molar radioactivity].
Kinetic parameters of enzymatic and non-enzymatic transformations of [3H]prostaglandin H2 (PGH2) were determined; the maximum yield of [3H]PGD2 being obtained at the keobs/koobs ratio equal to 10. The two-stage enzymatic synthesis of [3H]PGD2 with high molar radioactivity (3.15 TBq/mmol) from [3H]arachidonic acid carried out. Its identity in properties to the natural PGD2 was shown in experiments on the inhibition of ADP-induced aggregation of thrombocytes and on enzymatic oxidation with 15-hydroxyprostaglandin dehydrogenase.
Shram
S I
SI
Vrzheshch
P V
PV
Bobrovich
O A
OA
Tatarintsev
A V
AV
Shevchenko
V P
VP
Miasoedov
N F
NF
D000244:Adenosine Diphosphate / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000378:metabolism; D006911:Hydroxyprostaglandin Dehydrogenases / Q000378:metabolism; D007700:Kinetics; D010975:Platelet Aggregation Inhibitors; D015230:Prostaglandin D2 / Q000302:isolation & purification* / Q000378:metabolism; D011851:Radioactivity; D051381:Rats; D014316:Tritium
D010975:Platelet Aggregation Inhibitors; D014316:Tritium; D000244:Adenosine Diphosphate; D006911:Hydroxyprostaglandin Dehydrogenases; D015230:Prostaglandin D2
Poluchenie i svoĭstva mechennogo tritiem prostaglandina D2 s vysokoĭ moliarnoĭ radioaktivnost'iu.