Detection of human immunodeficiency virus (HIV) and human lymphotropic virus (HTLV) type I or II dual infections by polymerase chain reaction.
To type the specific human T-lymphotropic virus (HTLV) coinfection in acquired immunodeficiency syndrome (AIDS) patients, we have utilized the polymerase chain reaction technique. The primer pairs employed for HTLV-I and HTLV-II are derived from pol and env regions and gag region for human immunodeficiency virus (HIV). The data indicate that the selected primer pairs are specific for each virus and are able to detect HTLV-I and -II coinfection in lymphoid cells established from AIDS patients.
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Retrovirus Diseases Branch Centers for Disease Control, Atlanta, Georgia 30333.
Srinivasan
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D000163:Acquired Immunodeficiency Syndrome / Q000175:diagnosis; D001483:Base Sequence; D002460:Cell Line; D004259:DNA-Directed DNA Polymerase; D005814:Genes, Viral; D006678:HIV / Q000235:genetics*; D015658:HIV Infections / Q000175:diagnosis*; D015490:HTLV-I Infections / Q000175:diagnosis*; D015491:HTLV-II Infections / Q000175:diagnosis*; D015368:Human T-lymphotropic virus 1 / Q000235:genetics*; D015367:Human T-lymphotropic virus 2 / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods*
D015345:Oligonucleotide Probes; D004259:DNA-Directed DNA Polymerase