Determination of aflatoxin concentrations in peanut butter by enzyme-linked immunosorbent assay (ELISA): study of three commercial ELISA kits.
Sixteen United Kingdom analytical laboratories participated in an evaluation of 3 commercially available enzyme-linked immunosorbent assay (ELISA) kits for analysis of aflatoxin in peanut butter. Each laboratory was sent 3 sets of 10 randomly numbered samples of peanut butter. Each set consisted of 5 pairs of undisclosed duplicates. Four of the sets of duplicates were naturally contaminated butters with "target" aflatoxin values (estimated by liquid chromatography) between 8 and 81 micrograms/kg. The fifth pair was a blank peanut butter containing approximately 3 micrograms/kg of total aflatoxins. A statistical treatment of the results of the study is presented, together with discussion of the relative merits of the different kits.
Patey
A L
AL
Ministry of Agriculture, Fisheries and Food, Food Science Laboratory, Norwich, U.K.
Sharman
M
M
Wood
R
R
Gilbert
J
J
D000348:Aflatoxins / Q000032:analysis*; D010367:Arachis / Q000032:analysis*; D002853:Chromatography, Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D007118:Immunoassay; D011933:Reagent Kits, Diagnostic
D000348:Aflatoxins; D011933:Reagent Kits, Diagnostic