Sheep keratins: characterization of cDNA clones for the glycine + tyrosine-rich wool proteins using a synthetic probe.
cDNA clones coding for the high-glycine + tyrosine (HGT) proteins of sheep wool keratin have been isolated and sequenced. Clones were identified using a 25-base synthetic oligonucleotide probe, deduced from the amino acid sequence of a HGT protein present at about 0.4% in the wool fibre. Southern and Northern blot analysis suggest that the gene is present as a single copy in the genome and is transcribed as an mRNA species, 600 bases in size.
Kuczek
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Rogers
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D000818:Animals; D001483:Base Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA / Q000302:isolation & purification*; D005998:Glycine / Q000032:analysis*; D007633:Keratins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000302:isolation & purification; D012756:Sheep / Q000235:genetics; D014443:Tyrosine / Q000032:analysis*; D014935:Wool / Q000032:analysis*
D012333:RNA, Messenger; D014443:Tyrosine; D007633:Keratins; D004247:DNA; D005998:Glycine