Immunosuppression and in vivo T cell function in granulomatous inflammation.
Subcutaneous grafts of fresh or freeze-dried hepatic schistosome egg granulomas in inbred mice caused increased numbers of total cells, T cell subsets, and elevated DNA synthesis in regional lymph nodes. This in vivo T cell activation was not apparent by in vitro tests. Spleen cells were unaffected both in vivo and in vitro. Immunosuppressive therapy with ciclosporin or monoclonal antibodies to T cell subsets profoundly depressed the T cell numbers and function, even when primed by a prior graft. Subsequent grafting partly reversed these effects, and T cells became activated. The findings indicate that in vitro assessment of activation and suppression of T cell function does not necessarily correspond to the events occurring in vivo.
Fujioka
A
A
Department of Dermatology, University of California, San Francisco.
Pincelli
C
C
Hashimoto
A
A
Fukuyama
K
K
Epstein
W L
WL
D000818:Animals; D000950:Antigens, Ly / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D003524:Cyclosporins / Q000494:pharmacology; D006099:Granuloma / Q000276:immunology*; D007165:Immunosuppression Therapy; D007958:Leukocyte Count; D008198:Lymph Nodes / Q000166:cytology; D051379:Mice; D008819:Mice, Nude; D012555:Schistosomiasis mansoni / Q000276:immunology*; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology*
D000950:Antigens, Ly; D003524:Cyclosporins