title: Detection of the common acute lymphoblastic leukaemia antigen (CALLA) on B cells from human fetal tissues. A multiple phenotypic characterization.
abstract: Fetal bone marrow liver and spleen of gestational age 15-20 weeks contain CALLA+, HLA-DR+ lymphoid cells. We show that a proportion of them expresses surface membrane immunoglobulins (SIg) as well as B cell differentiation antigens. A multiple phenotypic analysis reveals that CALLA+ fetal B cells are: HLA-DR+, SIg+, FMC8+, BA1+, Y29.55+ or Y29.55-, B2+, TdT-. Tissue specific phenotypic differences concern the expression of B7 and HLA-DC on spleen but not on bone marrow B cells. This study indicates that the distribution of the CALL antigen across the B cell committed lineage is much wider in fetal than neonatal life since CALLA+B cells have not yet been detected in bone marrow and peripheral blood of normal infants and adults. In addition, the interpretation of our phenotypic data suggest that fetal bone marrow B cells are more immature than those present in the spleen, thus, further supporting the evidence that the bone marrow is the organ of B cell lymphopoiesis.
authors.forename: D
authors.initials: D
authors.lastname: Delia
authors.forename: G
authors.initials: G
authors.lastname: Cattoretti
authors.forename: A
authors.initials: A
authors.lastname: Bonati
authors.forename: S
authors.initials: S
authors.lastname: Villa
authors.forename: F
authors.initials: F
authors.lastname: De Braud
authors.forename: M
authors.initials: M
authors.lastname: Buscaglia
mesh_terms: D000951:Antigens, Neoplasm / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology*; D001853:Bone Marrow / Q000196:embryology / Q000276:immunology; D000939:Epitopes / Q000032:analysis; D005333:Fetus / Q000276:immunology*; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006801:Humans; D007945:Leukemia, Lymphoid / Q000276:immunology*; D008099:Liver / Q000196:embryology / Q000276:immunology; D015260:Neprilysin; D010641:Phenotype; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D013154:Spleen / Q000196:embryology / Q000276:immunology
chemical_list: D000951:Antigens, Neoplasm; D000939:Epitopes; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D011947:Receptors, Antigen, B-Cell; D015260:Neprilysin