title: A mononuclear cell-derived histamine releasing factor in asthmatic patients. II. Activity in vivo.
abstract: In an attempt to study the role of lymphokines in asthma, lymphocytes from asthmatic patients were stimulated in vitro with skin test-positive allergen (dust mite) or with non-specific mitogen-phytohaemagglutinin for 4 h, then washed carefully and cultured alone for 16 h. Cell-free supernatants were subsequently collected and applied in the basophil histamine release test in vitro and in bronchial provocation test and skin prick test in vivo. Supernatant of non-stimulated lymphocytes from asthmatic patients released significant amounts of histamine from basophils. Stimulation of lymphocytes with specific allergen or phytohaemagglutinin augmented the supernatant-induced histamine release. Lymphocyte supernatants from the majority of patients also induced bronchoconstriction and elicited skin wheal and flare reaction in vivo.
authors.forename: R
authors.initials: R
authors.lastname: Alam
authors.forename: J
authors.initials: J
authors.lastname: Rozniecki
mesh_terms: D001249:Asthma / Q000276:immunology*; D001491:Basophils / Q000276:immunology; D001985:Bronchial Provocation Tests; D006636:Histamine Release; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000276:immunology*; D008222:Lymphokines / Q000302:isolation & purification*; D012882:Skin Tests
chemical_list: D008222:Lymphokines