N-ras mutation in chemically induced rat brain tumour.
Rat brain tumour was induced by treatment with N-ethyl-nitrosourea. Using Southern blot analysis, restriction fragment length polymorphism of N-ras gene was identified. Comparative studies showed that new restriction site did not occur in the DNA of DMN induced renal and liver tumours. The data suggest that the mutation occurring may be specific to the "target" cell or to the structure of carcinogens.
Rády
P
P
Department of Hygiene Univ. Med. School of Debrecen, Hungary.
Kinsella
A R
AR
Fox
M
M
Arany
I
I
Kertai
P
P
D000818:Animals; D015139:Blotting, Southern; D001932:Brain Neoplasms / Q000139:chemically induced / Q000235:genetics*; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D005038:Ethylnitrosourea; D011905:Genes, ras / Q000235:genetics*; D009154:Mutation; D012150:Polymorphism, Restriction Fragment Length; D051381:Rats; D011916:Rats, Inbred F344
D015342:DNA Probes; D004273:DNA, Neoplasm; D005038:Ethylnitrosourea