title: Mechanism of action of colchicine. VI: Effect of colchicine on generation of leukotriene B4 by human polymorphonuclear leukocytes.
abstract: Three microtubule-disruptive agents (colchicine, oncodazole, and vinblastine) are shown to suppress ionophore A23187-induced generation of LTB4 in a dose-related manner. The slopes of the dose-response regression lines were similar for the three drugs. Trimethyl-colchicinic acid has no effect on LTB4 generation. The suppressive effect of colchicine (1 x 10(-6) M) can be blocked completely by taxol (1 x 10(-6) M) which promotes and stabilizes microtubule assembly. The data suggest that colchicine suppresses the generation of LTB4 via its effect on microtubules.
authors.affiliation: Department of Medicine, School of Medicine, University of California, Los Angeles, CA 90024.
authors.forename: Y
authors.initials: Y
authors.lastname: Ouyang
authors.forename: W
authors.initials: W
authors.lastname: Wang
authors.forename: S
authors.initials: S
authors.lastname: Bhuta
authors.forename: Y H
authors.initials: YH
authors.lastname: Chang
mesh_terms: D000470:Alkaloids / Q000494:pharmacology; D000001:Calcimycin / Q000037:antagonists & inhibitors / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D003078:Colchicine / Q000494:pharmacology*; D006801:Humans; D007975:Leukotriene B4 / Q000032:analysis / Q000096:biosynthesis*; D008854:Microscopy, Electron; D008870:Microtubules / Q000187:drug effects* / Q000648:ultrastructure; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D015739:Nocodazole / Q000494:pharmacology; D017239:Paclitaxel; D014527:Uric Acid / Q000494:pharmacology; D014747:Vinblastine / Q000494:pharmacology
chemical_list: D000470:Alkaloids; D007975:Leukotriene B4; D014527:Uric Acid; D000001:Calcimycin; D014747:Vinblastine; D017239:Paclitaxel; D015739:Nocodazole; D003078:Colchicine