Effects of AF64A on cholinergic neurotransmission in the sixth abdominal ganglion of the cockroach.
1. The effects of ethylcholine mustard aziridinium ion (AF64A) on the cholinergic neurotransmission in the sixth abdominal ganglion of the cockroach were studied electrophysiologically and morphologically. 2. The pre- and post-synaptic compound action potentials (CAPs) elicited via electrical stimulation of the presynaptic fibers were recorded extracellularly. 3. The amplitude of both CAPs was depressed by AF64A (50-400 microM) in a concentration- and time-dependent manner. 4. At a high concentration, they were abolished but 100 microM of carbachol still evoked the postsynaptic event. 5. Electron microscopic observation of AF64A-treated ganglia showed that nerve terminals containing small lucent vesicles could not be observed but those containing dense core or large granular vesicles changed only slightly in shape. 6. These results suggest that AF64A is selectively neurotoxic for the presynaptic cholinergic neurons in the sixth abdominal ganglion of the cockroach.
Morio
Y
Y
Department of Veterinary Pharmacology, College of Agriculture, University of Osaka Prefecture, Japan.
Yagasaki
O
O
D000005:Abdomen / Q000294:innervation; D000109:Acetylcholine / Q000502:physiology; D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D001388:Aziridines / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D002794:Choline / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000494:pharmacology; D003058:Cockroaches; D004558:Electric Stimulation; D005726:Ganglia, Parasympathetic / Q000187:drug effects*; D006426:Hemicholinium 3 / Q000494:pharmacology; D008297:Male; D008854:Microscopy, Electron; D009435:Synaptic Transmission / Q000187:drug effects*
D001388:Aziridines; D006426:Hemicholinium 3; C044894:ethylcholine aziridinium; D002794:Choline; D000109:Acetylcholine