Erythrocyte osmotic fragility and cation concentrations during experimentally induced bovine anaplasmosis.
1. The osmotic fragility, the concentrations of Na, K and Ca, the osmolality and the total ATPase activity of bovine erythrocytes from uninfected and Anaplasma marginale-infected bovines were studied in an attempt to correlate these parameters with the decrease in the cellular ATP concentration reported during bovine anaplasmosis. 2. The osmotic fragility found in infected bovine erythrocytes, at 0.52% NaCl, was about two times greater than that observed in non-infected bovines. The increase in osmotic fragility was directly related to the increase in intra-erythrocytic parasitemia. 3. The decrease in ATP concentration reported during bovine anaplasmosis could not be directly related to the increased fragility of these cells. The artificial depletion of erythrocytic ATP did not reproduce the same alteration in the osmotic response to NaCl. 4. The plasmatic and cytoplasmatic concentrations of Na, K and CA did not change significantly during bovine anaplasmosis, whereas the interior of the erythrocytes became hyperosmolal. 5. A. marginale-infected bovine erythrocyte membranes showed an increased ATPase activity when compared to control bovines. Parasite-enriched fractions also presented ATPase activity.
Silva
I M
IM
Departamento Biología Celular, Universidad Simón Bolívar, Caracas, Venezuela.
Hubsch
C
C
Ysern-Caldentey
M
M
D000251:Adenosine Triphosphatases / Q000097:blood; D000255:Adenosine Triphosphate / Q000097:blood; D000712:Anaplasmosis / Q000097:blood*; D000818:Animals; D002118:Calcium / Q000097:blood*; D002417:Cattle; D004910:Erythrocyte Membrane / Q000201:enzymology; D004912:Erythrocytes / Q000378:metabolism*; D008297:Male; D009994:Osmolar Concentration; D009996:Osmotic Fragility; D011188:Potassium / Q000097:blood*; D012964:Sodium / Q000097:blood*
D000255:Adenosine Triphosphate; D012964:Sodium; D000251:Adenosine Triphosphatases; D011188:Potassium; D002118:Calcium