Interferon beta increases expression of vimentin at the messenger RNA and protein levels in differentiated embryonal carcinoma (PSMB) cells.
Murine interferon beta increases expression of the 58-kDa intermediate filament protein vimentin by differentiated PSMB cells. Enhanced amounts of vimentin mRNA and protein have been detected using Northern hybridization and Western blotting techniques. Immunocytochemical analysis demonstrates the increased assembly of the protein into the intermediate filament network and its relocalization around the cell nucleus. Induction follows a defined time course, with peak protein levels 16 h post interferon addition, followed by a gradual decline over the next 36 h.
Alldridge
L C
LC
Department of Biology, University of Essex, Colchester, England.
O'Farrell
M K
MK
Dealtry
G B
GB
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002454:Cell Differentiation; D002460:Cell Line; D007370:Interferon Type I / Q000494:pharmacology*; D051379:Mice; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D013724:Teratoma; D014158:Transcription, Genetic / Q000187:drug effects; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D014746:Vimentin / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification
D007370:Interferon Type I; D012333:RNA, Messenger; D014746:Vimentin