beta-Amyloid precursor is a PEST protein.
The beta-amyloid peptide is generated by proteolytic processing of a family of beta-amyloid precursor proteins. Here we report that beta-amyloid precursor proteins have a primary structure motif known as a PEST sequence, which is predictive of the class of most protease-sensitive rapidly turning over proteins. Consistent with this, the precursors were extraordinarily susceptible to degradation by the calcium-dependent protease calpain I. The identification of beta-amyloid precursors as PEST sequence-containing proteins has implications for both the normal cellular function of beta-amyloid precursor proteins and the mechanisms regulating their expression and processing.
Siman
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Cephalon, Inc., West Chester, PA 19380.
Christoph
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G
D000595:Amino Acid Sequence; D000682:Amyloid / Q000378:metabolism; D016564:Amyloid beta-Protein Precursor; D000818:Animals; D001224:Aspartic Acid; D001923:Brain Chemistry; D002154:Calpain / Q000378:metabolism; D005971:Glutamates; D018698:Glutamic Acid; D008969:Molecular Sequence Data; D011392:Proline; D011480:Protease Inhibitors; D011498:Protein Precursors / Q000378:metabolism; D051381:Rats; D012694:Serine; D013912:Threonine
D000682:Amyloid; D016564:Amyloid beta-Protein Precursor; D005971:Glutamates; D011480:Protease Inhibitors; D011498:Protein Precursors; D013912:Threonine; D001224:Aspartic Acid; D018698:Glutamic Acid; D012694:Serine; D011392:Proline; D002154:Calpain