title: Choice of an optimal diluent for intravesical bacillus Calmette-Guerin administration.
abstract: The physical conditions, including diluent pH, salt concentration and duration of bacillus Calmette-Guerin attachment, were determined in in vitro binding assays for soluble and matrix fibronectin. Since soluble fibronectin may block attachment of bacillus Calmette-Guerin to matrix fibronectin in the bladder, the optimal conditions were determined under which matrix fibronectin-bacillus Calmette-Guerin binding was maximal and soluble fibronectin-bacillus Calmette-Guerin binding was minimal. These conditions, which were confirmed in vivo in the murine bladder model, included use of normal saline, pH 7 as diluent for bacillus Calmette-Guerin organisms, with retention of the bacillus Calmette-Guerin suspension for 2 hours.
authors.affiliation: Division of Urologic Surgery, Washington University School of Medicine, St. Louis, Missouri.
authors.forename: M A
authors.initials: MA
authors.lastname: Hudson
authors.forename: W J
authors.initials: WJ
authors.lastname: Catalona
authors.forename: J K
authors.initials: JK
authors.lastname: Ritchey
authors.forename: J
authors.initials: J
authors.lastname: Aslanzadeh
authors.forename: E J
authors.initials: EJ
authors.lastname: Brown
authors.forename: T L
authors.initials: TL
authors.lastname: Ratliff
mesh_terms: D000283:Administration, Intravesical; D000818:Animals; D001500:BCG Vaccine / Q000008:administration & dosage* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005353:Fibronectins / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D051379:Mice; D010592:Pharmaceutic Aids / Q000008:administration & dosage* / Q000378:metabolism; D012995:Solubility; D013997:Time Factors; D014316:Tritium
chemical_list: D001500:BCG Vaccine; D005353:Fibronectins; D007457:Iodine Radioisotopes; D010592:Pharmaceutic Aids; D014316:Tritium