[Impaired B lymphocyte function during open heart surgery].
Sequential in vitro lymphocyte function tests in 13 patients undergoing cardiac operation were performed to study B lymphocyte function following operation. Lymphocytes were stimulated with phytohemagglutinin (PHA), pokeweed mitogen (PWM) and Staphylococcus aureus Cowan 1 (SAC). Mitogen responses were measured by 3H-labeled thymidine incorporation. The SAC responses were significantly depressed following operation. Immunoglobulin secreting cells were measured by protein A plaque forming cell assay. The numbers of immunoglobulin secreting cells induced by PWM or SAC decreased remarkably at least as long as 3 day after the operation. The percentage of circulating B lymphocytes increased significantly postoperatively. This indicates that the B lymphocytes remaining after the operation were functionally impaired.
Hirai
K
K
Shimomura
T
T
Ishizaka
S
S
Shimokawa
M
M
Kitaguchi
K
K
Yamagishi
N
N
Kuro
M
M
Okuda
T
T
D000921:Antibody-Producing Cells / Q000166:cytology; D001402:B-Lymphocytes / Q000276:immunology*; D006348:Cardiac Surgical Procedures; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged