The design of fluorescent probes which bind to the active centre of guanidinobenzoatase. Application to the location of cells possessing this enzyme.
Cells possessing a known enzymic activity may be located by fluorescent probes designed to act as competitive inhibitors of this enzyme. We have prepared a series of dansyl N-substituted guanidino derivatives which bind to the active centre of guanidinobenzoatase. 9-Aminoacridine also acts as a competitive inhibitor and behaves similarly to these guanidino derivatives. These fluorescent probes have been used to locate tumour cells possessing this enzyme in thin sections of fixed tissue by employing fluorescent microscopy.
Steven
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Griffin
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Al-Ahmad
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RK
D000585:Aminacrine / Q000138:chemical synthesis; D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002265:Carboxylic Ester Hydrolases / Q000037:antagonists & inhibitors*; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology; D003619:Dansyl Compounds / Q000138:chemical synthesis; D010450:Endopeptidases; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D006801:Humans; D051379:Mice; D009374:Neoplasms, Experimental / Q000201:enzymology; D011817:Rabbits; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling; D001749:Urinary Bladder Neoplasms / Q000201:enzymology
D003619:Dansyl Compounds; D005456:Fluorescent Dyes; D000585:Aminacrine; D002265:Carboxylic Ester Hydrolases; C037288:guanidinobenzoate esterase; D010450:Endopeptidases