title: The carbohydrate moiety of aminopeptidase N of rabbit intestinal brush-border membrane.
abstract: Endoglycosidase F was used to eliminate the N-linked complex glycans from intestinal aminopeptidase N. The glycans which were probably O-linked remaining after the endoglycosidase F treatment exhibited the human blood group A and H determinants expressed in enzymes from A+ or A- rabbits, respectively. The molecular mass estimation of the two types of glycans by SDS-polyacrylamide gel electrophoresis and the sugar composition of aminopeptidase from A+ and A- rabbits strongly suggested the presence of eight N-linked complex glycans and two O-linked oligosaccharides bearing the human group antigenicity.
authors.forename: D
authors.initials: D
authors.lastname: Massey
authors.forename: S
authors.initials: S
authors.lastname: Maroux
mesh_terms: D000017:ABO Blood-Group System / Q000276:immunology; D000626:Aminopeptidases / Q000032:analysis*; D000818:Animals; D018826:CD13 Antigens; D002236:Carbohydrate Conformation; D002241:Carbohydrates / Q000032:analysis* / Q000276:immunology; D000939:Epitopes / Q000032:analysis; D006026:Glycoside Hydrolases; D007422:Intestines / Q000201:enzymology*; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D008871:Microvilli / Q000201:enzymology; D009844:Oligosaccharides / Q000032:analysis; D011134:Polysaccharides / Q000032:analysis; D011817:Rabbits
chemical_list: D000017:ABO Blood-Group System; D002241:Carbohydrates; D000939:Epitopes; D009844:Oligosaccharides; D011134:Polysaccharides; D006026:Glycoside Hydrolases; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D000626:Aminopeptidases; D018826:CD13 Antigens