Syngeneic sensitization of mouse lymphocytes on monolayers of thyroid epithelial cells. VII. Generation of thyroid-specific cytotoxic effector cells.
During primary syngeneic sensitization of lymphocytes on monolayers of thyroid epithelial cells (TEC), lymphoblasts are generated as assessed by thymidine uptake. When these thyroid-sensitized lymphoblasts are deposited onto 51Cr labeled TEC used as target cells, syngeneic cytotoxicity (SC) occurs after 5 hr. The effector cell of this SC is a T lymphocyte, belonging to the Lyt-2 T-cell subset. Moreover, blocking experiments demonstrated that SC involves class I major histocompatibility complex and thyroglobulin (Tg) antigens. These data demonstrate that SC can be compared to the classical hapten or virus self-mediated cytotoxicity, but in this case Tg plays the role of these agents. The in vivo relevance of this SC is discussed.
Salamero
J
J
Charreire
J
J
D000818:Animals; D003601:Cytotoxicity Tests, Immunologic; D004847:Epithelial Cells; D004848:Epithelium / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology*; D007114:Immunization; D007074:Immunoglobulin G / Q000502:physiology; D018828:Immunoglobulins, Thyroid-Stimulating; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D013954:Thyroglobulin / Q000276:immunology; D013961:Thyroid Gland / Q000166:cytology* / Q000276:immunology
D000939:Epitopes; D006183:H-2 Antigens; D007074:Immunoglobulin G; D018828:Immunoglobulins, Thyroid-Stimulating; D013954:Thyroglobulin