title: Determination of homocysteine in plasma by ion-exchange chromatography.
abstract: A rapid ion-exchange chromatographic method for determination of total homocysteine in plasma is presented. The method has a coefficient of variation of approximately 5%. A comparison with a slower method previously used in our laboratory showed a high correlation (r = 0.993). The risk of oxidation of homocysteine during the chromatography and the necessary level of dithiotreithol to prevent oxidation are also established.
authors.affiliation: Department of Clinical Chemistry, University Hospital, Lund, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Andersson
authors.forename: L
authors.initials: L
authors.lastname: Brattström
authors.forename: A
authors.initials: A
authors.lastname: Isaksson
authors.forename: B
authors.initials: B
authors.lastname: Israelsson
authors.forename: B
authors.initials: B
authors.lastname: Hultberg
mesh_terms: D002852:Chromatography, Ion Exchange / Q000379:methods; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D008715:Methionine / Q000097:blood; D010084:Oxidation-Reduction
chemical_list: D006710:Homocysteine; D008715:Methionine