Two UV-sensitive targets in dorsoanterior specification of frog embryos.
Previous work has shown that ultraviolet (UV) irradiation of fertilized frog eggs yields embryos that lack dorsal and anterior structures. The eggs fail to undergo the cortical/cytoplasmic rotation that specifies dorsoventral polarity, and they lack an array of parallel microtubules associated with the rotation. These eggs can be rescued by tilting with respect to gravity, and normal dorsoanterior development occurs. We find here that UV irradiation of Xenopus prophase I oocytes or Rana metaphase I oocytes also causes the dorsoanterior deficient syndrome, but the UV target is different from that in fertilized eggs. Tilting eggs, irradiated as oocytes, with respect to gravity, does not rescue dorsoanterior development, although lithium treatment does. The UV dose required to produce dorsoanterior deficiency for Rana metaphase I oocytes is much less than that for fertilized eggs, and the oocytes can form the array of parallel microtubules and undergo the cortical/cytoplasmic rotation after fertilization. Despite these features of normal development, no dorsoanterior structures form. While the UV target in fertilized eggs is thought to be the parallel microtubules (Elinson & Rowning, 1988; Devl Biol. 128, 185-197), the UV target in the oocytes may be a dorsal determinant.
Elinson
R P
RP
Department of Zoology, University of Toronto, Ontario, Canada.
Pasceri
P
P
D000818:Animals; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000502:physiology / Q000528:radiation effects*; D005260:Female; D008094:Lithium / Q000494:pharmacology; D008870:Microtubules / Q000187:drug effects / Q000528:radiation effects / Q000648:ultrastructure; D009865:Oocytes / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects*; D011894:Rana pipiens; D014466:Ultraviolet Rays; D014982:Xenopus laevis; D015053:Zygote / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects
D008094:Lithium