A micro-kininogenase assay for studies of kallikrein in renal micropuncture/microperfusion.
We report the development of a micro-kininogenase assay suitable in studying the dynamics of kallikrein at intranephron segmental level. The detection limit is 119 fg or 2.6 attomoles of kallikrein. Activation of microquantities of kallikrein is possible with the use of Triton X-100. Because of its extremely high sensitivity and reproducibility the assay is likely to also prove useful in physiological studies in which only very small amounts of kallikrein containing samples can be obtained.
Oza
N B
NB
Evans Memorial Dept. of Clinical Research, University Hospital, Boston University Medical Center, MA 02118.
Murphy
C M
CM
Kaufman
J S
JS
Beasley
D
D
Levinsky
N G
NG
D000818:Animals; D001707:Biopsy, Needle; D004789:Enzyme Activation; D007610:Kallikreins / Q000378:metabolism* / Q000652:urine; D007668:Kidney / Q000201:enzymology*; D007700:Kinetics; D007705:Kinins / Q000302:isolation & purification / Q000378:metabolism; D008832:Microchemistry; D010477:Perfusion; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats
D007705:Kinins; D007610:Kallikreins