The role of xenoantigen in the human autologous mixed lymphocyte reaction: ability to respond in the absence of exposure to xenoantigen depends on the method of T-cell preparation.
Recent reports have suggested that the autologous mixed lymphocyte reaction (AMLR) is attributable to contaminating xenoantigen. T-cell populations separated from peripheral blood mononuclear cells (PBM) over Percoll fail to produce an AMLR in the absence of xenoantigen. However, T-cell populations that do not adhere to plastic and to nylon wool will in the absence of added xenoantigen produce an AMLR comparable to that obtained with E-rosetted populations. In the nonadherent cells separated sequentially by passage through nylon wool and Percoll, the presence of two lymphocyte subpopulations was demonstrated. One is xenoantigen dependent, the other xenoantigen independent in the AMLR. Further, the xenoantigen-independent population may provide help to the dependent population. In the previous reports with Percoll-separated cells that attributed the AMLR to contaminating xenoantigen, the xenoantigen-independent T-cell population was excluded from the responding fraction.
Naides
S J
SJ
Redelman
D
D
Zvaifler
N J
NJ
D000818:Animals; D000941:Antigens / Q000502:physiology*; D002469:Cell Separation / Q000379:methods; D000939:Epitopes; D005260:Female; D006801:Humans; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D009757:Nylons; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*; D014935:Wool
D000941:Antigens; D000939:Epitopes; D009757:Nylons