[In vitro stimulation of alkaline phosphatase and gamma- glutamyltransferase in the kidney of hypophysectomized rat by the mixture of 1,25 and 24,25 dihydroxycholecalciferols].
The present study was undertaken to investigate the in vitro effects of two cholecalciferol metabolites 1,25(OH)2CC and 24,25(OH)2CC on the renal cortex alkaline phosphatase (ALP), gamma-glutamyltransferase (GGT) and acid phosphatase (ACP) activities in hypophysectomized rats. We found that both 1,25-CC and 24,25-CC stimulated ALP and GGT. An additive effect was obtained on ALP and GGT after incubation with one with the other metabolites. When 1,25-CC was used alone, it increased ACP; in presence of 24,25-CC, this stimulating effect disappeared. In the rat, together 1,25-CC and 24,25-CC correct in vitro ALP and GGT activities decreased by hypophysectomy. Physiological signification of these effects remains again unknown.
Do Thanh
X
X
Laboratoire de Physiologie, Centre Hospitalier Universitaire, UER des Sciences Medicales et Pharmaceutiques, Angers, France.
Planchenault
P
P
Girault
A
A
Prelot
M
M
D015650:24,25-Dihydroxyvitamin D 3 / Q000494:pharmacology*; D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D002117:Calcitriol / Q000494:pharmacology*; D004347:Drug Interactions; D005260:Female; D007016:Hypophysectomy; D007668:Kidney / Q000187:drug effects / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D005723:gamma-Glutamyltransferase / Q000378:metabolism*
D015650:24,25-Dihydroxyvitamin D 3; D005723:gamma-Glutamyltransferase; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D002117:Calcitriol
Stimulation in vitro de la phosphatase alcaline et de la gamma-glutamyltransférase du rein de rat hypophysectomisé par le mélange des 1,25 et 24,25 dihydroxycholécalciférols.