[Analysis of trophoblast subpopulations on touch smears of chorionic villi during normal pregnancy].
Trophoblasts of normal human pregnancy are classified into syncytiotrophoblasts (STs), cytotrophoblasts (CTs) and intermediate trophoblasts (ITs) which exist in cell columns and so forth. We analyzed the trophoblast subpopulations which appear on touch smears of chorionic villi morphologically and immunohistochemically, using the uterine contents of 37 cases of induced abortion. Troma 1, a rat monoclonal antibody, was utilized as a trophoblast marker and a mouse monoclonal antibody to HLA-A,B,C was employed to discriminate ITs from CTs. The results were as follows: 1. Two sorts of cells were positive for Troma 1 and therefore were considered to be trophoblastic: Multinuclear cells of various sizes and relatively large mononuclear cells. 2. Multinuclear trophoblasts were thought to be STs because of their characteristic cytomorphology and negative reaction for HLA-A,B,C. 3. The lower the gestational age was, the more ITs were observed. 4. The cellular and nuclear size of ITs varied and their chromatin was coarsely granular with aggregates. In addition one or more marked nucleoli were noted. Consequently it would be important to take care not to misdiagnose ITs as trophoblasts of a malignant nature.
Sasaki
T
T
Department of Perinato-Gynecology, Kagawa Medical School.
Sasagawa
M
M
Mochizuki
M
M
Yasuda
M
M
Yamazaki
T
T
Kurose
T
T
Tanaka
K
K
Hando
T
T
D000911:Antibodies, Monoclonal; D015193:Chorionic Villi Sampling / Q000379:methods; D005260:Female; D005865:Gestational Age; D006680:HLA Antigens / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D011247:Pregnancy / Q000502:physiology*; D014327:Trophoblasts / Q000032:analysis / Q000166:cytology*
D000911:Antibodies, Monoclonal; D006680:HLA Antigens