title: An evaluation of the Coulter VCS differential counter.
abstract: An evaluation of the Coulter VCS (volume, conductivity, light scatter) automated differential counter demonstrated satisfactory correlations with manual 800-cell differential counts for neutrophils, lymphocytes, monocytes and eosinophils. For the detection of abnormal cells, 12.2% of samples gave false-negative results on the VCS, and 13.4% gave false-positive results. However, only 0.8% of the false-negatives would be expected to be picked up by a standard 100-cell manual differential count. Carry-over accorded to manufacturer's specifications and throughput was 60 samples/h. Reference normal ranges have been established and the instrument's precision and performance with leucopenic and neonatal blood samples assessed.
authors.affiliation: Department of Haematology, Middlesex Hospital Medical School, London.
authors.forename: D F
authors.initials: DF
authors.lastname: Barnard
authors.forename: S A
authors.initials: SA
authors.lastname: Barnard
authors.forename: A B
authors.initials: AB
authors.lastname: Carter
authors.forename: K G
authors.initials: KG
authors.lastname: Patterson
authors.forename: A
authors.initials: A
authors.lastname: Yardumian
authors.forename: S J
authors.initials: SJ
authors.lastname: Machin
mesh_terms: D000328:Adult; D001491:Basophils; D003201:Computers; D004804:Eosinophils; D005069:Evaluation Studies as Topic; D006402:Hematologic Diseases / Q000097:blood; D006801:Humans; D007231:Infant, Newborn; D007958:Leukocyte Count / Q000295:instrumentation*; D007970:Leukopenia / Q000097:blood; D008214:Lymphocytes; D008875:Middle Aged; D009000:Monocytes; D009504:Neutrophils; D012016:Reference Values; D013997:Time Factors