Preliminary investigation of ion mobility spectrometry after capillary electrophoretic introduction.
Using standard capillary electrophoretic and ion mobility methods, several electrospray interface designs were investigated for the capillary electrophoretic introduction of samples into the ion mobility spectrometer. Of the interfaces investigated, the flow assisted interface and the direct coupled interface showed the most promise. These preliminary experiments were encouraging. The ion mobility spectrometer coupled with a capillary electrophoretic introduction system operated with excellent separation efficiency and ion mobility reproducibility. Using tetrabutylammonium iodide, the number of theoretical plates for the spectrometer was calculated to be 3.10(3) and reduced mobilities were found to be reproducible with a relative standard deviation of 1.43%. Because of the desire to hold the spectrometer as hot as possible, the solvent would often vaporize in the interface, creating an unstable spray and inhomogeneities in the electrophoretic field. More work is needed to improve the spray process which contributed to the overall noise of the system and to eliminate the phenomenon of solvent vaporization which limited the reproducibility of electrophoretic migration times.
Hallen
R W
RW
Department of Chemistry, Washington State University, Pullman 99164-4630.
Shumate
C B
CB
Siems
W F
WF
Tsuda
T
T
Hill
H H
HH
D004586:Electrophoresis / Q000379:methods*; D007477:Ions; D013057:Spectrum Analysis / Q000379:methods*
D007477:Ions