[Modification by azocombination of the catalytic properties of ribonucleases].
Using pancreatic RNAase and RNAase from Act. rimosus as models, the effect of modification by azocombination on the catalytic properties of enzymes were studied. It was shown that RNAases binding to soluble dextran did not cause any significant changes in their major catalytic properties, when polymeric RNA was used as a substrate. At the same time, the physico-chemical properties of the modified enzymes may result in changes in the catalytic properties in a reaction with low molecular weight substrates. Evidence for this observation can be obtained from the increase in the synthetic activity of modified pancreatic RNAase as compared to the hydrolase activity in the dinucleotide synthesis reaction.
Kalacheva
N V
NV
Kurinenko
B M
BM
D000190:Actinomyces / Q000201:enzymology; D000818:Animals; D002417:Cattle; D003911:Dextrans; D004800:Enzymes, Immobilized / Q000378:metabolism*; D008970:Molecular Weight; D009843:Oligoribonucleotides / Q000378:metabolism; D010179:Pancreas / Q000201:enzymology; D012313:RNA / Q000378:metabolism; D006163:Ribonuclease T1 / Q000378:metabolism; D012259:Ribonuclease, Pancreatic / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism*; D013379:Substrate Specificity
D003911:Dextrans; D004800:Enzymes, Immobilized; D009843:Oligoribonucleotides; D012313:RNA; D012260:Ribonucleases; D006163:Ribonuclease T1; D012259:Ribonuclease, Pancreatic
Vliianie modifikatsii azosochetaniem na kataliticheskie svoĭstva RNKaz.