title: HTLV-positive and -negative T-cell lymphomas. Morphological and immunohistochemical differences between European and HTLV-positive Japanese T-cell lymphomas.
abstract: A total of 56 cases of malignant lymphoma presumed to be of peripheral T-cell origin were investigated with regard to histological and immunohistochemical features. The goal of the study was to determine whether virus-associated T-cell lymphomas can be morphologically or immunohistochemically distinguished from presumably virus-negative T-cell lymphomas. The cases came from endemic and non-endemic regions of Japan, the United Kingdom (including 4 Caribbean cases) and the Federal Republic of Germany. Sera of all Japanese and Caribbean patients and 8 German patients were tested for antibodies to adult T-cell leukaemia virus-associated antigen HTLV-A. In all cases sections were examined blind by 5 well-trained histopathologists. In most cases cryostat sections could be prepared from fresh tissue specimens and stained with a large panel of monoclonal antibodies. All HTLV-A-positive cases were morphologically classifiable as the pleomorphic type of T-cell lymphoma. Approximately 70% of the tested cases of pleomorphic T-cell lymphoma, however, showed a positive serum reaction for HTLV-A. All other types of peripheral T-cell lymphoma (T-immunoblastic lymphoma, chronic lymphocytic leukaemia of T type, T-zone lymphoma, "AILD type" and lymphoepithelioid cell lymphoma) were HTLV-A-negative and mostly observed in European patients. Thus virus-associated T-cell lymphomas appear to be invariably of the pleomorphic type; but pleomorphism is not specific to HTLV-A-positive cases. This was also evident from the results of an experiment in which 2 Japanese histopathologists attempted to recognize HTLV-A positivity in a blind study of pleomorphic T-cell lymphomas. A maximum of about 80% of cases were correctly identified, with about 10% false-positive diagnoses (in HTLV-A-negative or presumably negative cases) and 10% false-negative diagnoses. The immunohistochemical analysis revealed not only many common features but also 2 distinct differences between HTLV-A-positive and -negative T-cell lymphomas. All but one of the HTLV-A-positive cases showed reactivity with anti-Tac and all cases in the virus-positive group were negative for TU14. All other cases were Tac-negative and approximately 65% of these cases exhibited reactivity with TU14. Preliminary cytogenetic observations suggest that there are also differences in specific chromosome aberrations.
authors.forename: K
authors.initials: K
authors.lastname: Lennert
authors.forename: M
authors.initials: M
authors.lastname: Kikuchi
authors.forename: E
authors.initials: E
authors.lastname: Sato
authors.forename: T
authors.initials: T
authors.lastname: Suchi
authors.forename: A G
authors.initials: AG
authors.lastname: Stansfeld
authors.forename: A C
authors.initials: AC
authors.lastname: Feller
authors.forename: M L
authors.initials: ML
authors.lastname: Hansmann
authors.forename: H K
authors.initials: HK
authors.lastname: Müller-Hermelink
authors.forename: E
authors.initials: E
authors.lastname: Gödde-Salz
mesh_terms: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D002467:Cell Nucleus / Q000473:pathology; D003593:Cytoplasm / Q000473:pathology; D017977:Deltaretrovirus / Q000276:immunology; D005060:Europe; D005189:False Positive Reactions; D006651:Histocytochemistry; D006801:Humans; D007120:Immunochemistry; D007564:Japan; D008223:Lymphoma / Q000145:classification / Q000276:immunology / Q000473:pathology*; D012192:Retroviridae Infections / Q000276:immunology* / Q000473:pathology; D013194:Staining and Labeling; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology*; D014900:West Indies
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000951:Antigens, Neoplasm; D000954:Antigens, Surface