cDNA cloning of the nucleocapsid and nucleocapsid-associated protein genes of mumps virus.
cDNA clones of the mumps virus N and P messenger RNAs were isolated from an infected cell cDNA library. The N and P clones selected the two predominant polyadenylated RNAs found in mumps virus-infected cells with mol. wt. of 0.69 X 10(6) and 0.51 X 10(6), respectively. In addition, clones of the P gene hybridized to and selected mRNAs of higher mol. wt. probably representing polycistronic transcripts of the mumps genome. Hybrid-select translation experiments confirmed the specificity of the clones as representing the nucleocapsid (N) and nucleocapsid-associated protein (P) genes.
Curran
J A
JA
Quinn
J P
JP
Hoey
E M
EM
Martin
S J
SJ
Rima
B K
BK
D002213:Capsid / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D009109:Mumps virus / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000235:genetics; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics*
D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D011061:Poly A; D012313:RNA; D004247:DNA