Interleukin 2-mediated induction of lytic activity in a cloned murine CTL line.
We have analyzed the ability of interleukin 2 (IL 2) to induce lytic activity within a cloned murine H-2Dd-specific CTL line. Weakly lytic CTL harvested 6 to 7 days after previous stimulation with irradiated DBA/2J spleen cells and conditioned medium from secondary MLC (MLC SN) could be reactivated to high antigen-specific lytic activity with highly purified gibbon IL 2 or E. coli-produced human recombinant DNA IL 2. Dose-response curves with IL 2 and MLC SN suggest that IL 2 may be the principal detectable activity in MLC SN that is active on these CTL. Doses of IL 2 or MLC SN that were saturating for the induction of lytic activity were suboptimal for the expression of DNA synthesis measured by 3HTdR incorporation. This is consistent with a mechanism in which different threshold IL 2 concentrations are required to induce these two biologic responses. Finally, we show that IFN-gamma has little effect on the expression of lytic activity either alone or in combination with IL 2 in this bioassay.
Howe
R C
RC
Milstone
D S
DS
Ratliff
T L
TL
Russell
J H
JH
D000042:Absorption; D000818:Animals; D002460:Cell Line; D002999:Clone Cells / Q000276:immunology; D003470:Culture Media; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D006801:Humans; D006922:Hylobates; D007371:Interferon-gamma / Q000096:biosynthesis / Q000494:pharmacology; D007376:Interleukin-2 / Q000235:genetics / Q000502:physiology*; D007519:Isoantigens / Q000235:genetics / Q000276:immunology; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D013154:Spleen / Q000166:cytology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000378:metabolism
D003470:Culture Media; D000939:Epitopes; D007376:Interleukin-2; D007519:Isoantigens; D007371:Interferon-gamma