Cloning of the gene for cell-surface protein antigen A from Streptococcus sobrinus (serotype d).
A gene library of Strep. sobrinus B13N (serotype d) chromosomal DNA was constructed in Escherichia coli, with the bacteriophage vector lambda L47.1. A recombinant phage, lambda MDSM49, containing a 15.5 kb DNA insert, directed the expression of a 210 kDa antigenic protein. The recombinant 210 kDa protein was shown by Western blot analysis to be identical with cell-surface protein antigen A (spaA) from a serotype g strain. However, the restriction patterns of a subclone plasmid, pMD51, from lambda MDSM49 differed from those of serotype g strain. The cell-surface protein antigen I/II from serotype c Streptococcus mutans is a potential immunogen for vaccination against dental caries and corresponds to the spaA from serotype d and g strains. A recombinant clone, pDM51, will be a useful tool for serological and molecular biological studies. The recombinant spaA provides useful material for assessment of its diagnostic and immunogenic potential.
Abiko
Y
Y
Department of Biochemistry, Nihon University School of Dentistry, Chiba, Japan.
Hayakawa
M
M
Aoki
H
H
Saito
S
S
Takiguchi
H
H
D000942:Antigens, Bacterial / Q000235:genetics*; D000954:Antigens, Surface / Q000235:genetics*; D015153:Blotting, Western; D003001:Cloning, Molecular / Q000379:methods*; D004269:DNA, Bacterial; D015723:Gene Library; D005798:Genes, Bacterial; D009693:Nucleic Acid Hybridization; D013295:Streptococcus mutans / Q000235:genetics* / Q000276:immunology
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D004269:DNA, Bacterial