Monoclonal antibodies to the human laminin receptor recognize structurally distinct sites.
Laminin, a glycoprotein of basement membranes, binds to a specific receptor on the surface of neoplastic and non-neoplastic cells. The laminin receptor purified from human breast carcinoma plasma membranes was used as an antigen to generate two types of monoclonal antibodies (mAbs). Both types of mAbs bind to (a) the purified receptor coated on a solid phase; (b) isolated breast carcinoma plasma membranes; and (c) the surface of cultured MCF-7 human breast carcinoma cells by immunohistology. Using immunoblotting, both types of mAbs recognize a single 67 000 Dalton protein among all the proteins extracted from breast carcinoma plasma membranes. The mAbs differed in their ability to block binding of laminin to the plasma membrane receptor. Antibody LR1 inhibited virtually 100% of the specific binding of laminin to both the isolated human breast carcinoma plasma membranes or the living MCF-7 cells. In contrast, antibody LR2 had no effect on laminin binding under identical conditions. Thus, the two types of mAbs may recognize structurally distinct sites on the laminin receptor. These mAbs should be useful to dissect the biology and the molecular genetics of the laminin receptor.
Liotta
L A
LA
Horan Hand
P
P
Rao
C N
CN
Bryant
G
G
Barsky
S H
SH
Schlom
J
J
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D001943:Breast Neoplasms / Q000032:analysis* / Q000473:pathology; D002460:Cell Line; D002462:Cell Membrane / Q000032:analysis; D000939:Epitopes / Q000032:analysis*; D005260:Female; D006801:Humans; D007700:Kinetics; D007797:Laminin / Q000378:metabolism; D011971:Receptors, Immunologic / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D017474:Receptors, Laminin
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000939:Epitopes; D007797:Laminin; D011971:Receptors, Immunologic; D017474:Receptors, Laminin