Nucleotide sequence cleavages of manganese-bleomycin induced by reductant, hydrogen peroxide and ultraviolet light. Comparison with iron- and cobalt-bleomycins.
The prominent DNA breakages of bleomycin-Mn complex system were induced by reductant, hydrogen peroxide and ultraviolet light, and these three induction systems gave remarkably similar nucleotide sequence cleavage modes. The preferred DNA cleavage sites at guanine-cytosine(5'----3') and guanine-thymine(5'----3') sequences were appreciably comparable to those of the corresponding bleomycin-Fe complex systems, but not identical. In contrast, the bleomycin-Co complex system significantly degraded isolated DNA only by irradiation of ultraviolet light. The present results provide valuable information on the role of transition metals on DNA cleavage reaction of bleomycin.
Suzuki
T
T
Kuwahara
J
J
Goto
M
M
Sugiura
Y
Y
D001483:Base Sequence; D001761:Bleomycin / Q000494:pharmacology* / Q000031:analogs & derivatives; D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA / Q000528:radiation effects; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008623:Mercaptoethanol / Q000494:pharmacology*; D014466:Ultraviolet Rays
C030187:cobalt bleomycin; C023503:iron bleomycin; D001761:Bleomycin; D008623:Mercaptoethanol; C045115:manganese bleomycin; D004247:DNA; D006861:Hydrogen Peroxide