title: Monospecific antibodies against a synthetic peptide predicted from the alpha-3 nicotinic receptor cDNA inhibit binding of [3H]nicotine to rat brain nicotinic cholinergic receptor.
abstract: Polyclonal antibodies were raised against a synthetic decapeptide (designated S3) predicted from a segment of the alpha-3 subunit cDNA (amino acid residues 130-139) encoding the rat brain nicotinic cholinergic receptor. This segment was selected because it may be proximate to the nicotine/acetylcholine-binding site of the receptor (1). By radioligand binding assays and sucrose density gradient centrifugation, these monospecific antibodies were shown to inhibit the binding of [3H]nicotine to both the large molecular weight rat brain receptor (240 kDa) and to an SDS-disaggregated nicotine-binding subunit species (80 kDa), in a dose-dependent manner. The neutralizing effect of the anti-S3 antibodies supports the view that this region of the protein is closely related to the agonist binding site.
authors.affiliation: Endocrine-Neuroscience Laboratory, Minneapolis Medical Research Foundation, MN.
authors.forename: T C
authors.initials: TC
authors.lastname: Madhok
authors.forename: C C
authors.initials: CC
authors.lastname: Chao
authors.forename: S G
authors.initials: SG
authors.lastname: Matta
authors.forename: A
authors.initials: A
authors.lastname: Hong
authors.forename: B M
authors.initials: BM
authors.lastname: Sharp
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001921:Brain / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D004247:DNA / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D007700:Kinetics; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009538:Nicotine / Q000378:metabolism*; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000276:immunology; D051381:Rats; D011978:Receptors, Nicotinic / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D046911:Macromolecular Substances; D009842:Oligopeptides; D011978:Receptors, Nicotinic; D009538:Nicotine; D004247:DNA