[The role of protein phosphorylation in mast cell secretion, stimulated by concanavalin A. Connection to the cytoskeleton].
The role of cytoskeleton and protein phosphorylation in concanavalin A and phorbol ester (PMA) induced mast cell secretion was investigated. It was shown that the receptor coupled with lectin interacts with the cytoskeleton. When the ligand-receptor complex is formed, an increased phosphorylation of some proteins is induced. The same proteins are phosphorylated under the influence of PMA, a protein kinase C activator, thus suggesting that protein kinase C is involved in the regulation of mast cell exocytosis. The results obtained testify to the effect that the mechanism of mast cell degranulation induced by concanavalin A is due to modification of the cytoskeleton.
Dulin
N O
NO
Goncharenko
E N
EN
Kudriashov
Iu B
IuB
Graevskaia
E E
EE
Kravtsov
G M
GM
D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology*; D003599:Cytoskeleton / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D005453:Fluorescence; D006632:Histamine / Q000032:analysis; D007700:Kinetics; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D010703:Phorbol Esters / Q000494:pharmacology; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D051381:Rats
D010703:Phorbol Esters; D011506:Proteins; D003208:Concanavalin A; D006632:Histamine; D011493:Protein Kinase C
Rol' fosforilirovaniia belkov v sekretsii tuchnykh kletok, stimulirovannykh konkavalinom A. sviaz' s tsitoskeletom.