title: Natural anti-TNP antibodies from rainbow trout interfere with viral infection in vitro.
abstract: Normal and viral-infected rainbow trout (RT) were tested for serum antibody activity against self and nonself antigens. Particularly high titres of anti-trinitrophenyl (TNP) antibodies were noted, as in other fish species. To analyse this, the anti-TNP antibodies were isolated by affinity chromatography and their capacity to interfere with viral infection in vitro was studied. We selected RT fibroblasts as target cells, and two common pathogenic viruses in trout, a rhabdovirus, viral haemorrhagic septicaemia virus (VHS) and a birnavirus, the infectious pancreatic necrosis virus (IPN). Anti-TNP antibodies were examined for their capacity to neutralize VHS and IPN viruses. Data obtained show that the anti-TNP antibodies, even at high concentrations, only partially neutralized virus. In contrast, when anti-TNP antibodies were assayed for their protective activity using RT fibroblast cells infected with VHS or IPN viruses, results showed high protective activity, regardless of serum origin or of the virus used, when the antibodies were added to the cell culture after viral infection. Therefore, our experiments indicate that the protective activity does not seem to be due to a direct interaction of the antibodies with the viruses. It is suggested that virus-modified cell surface self structures exhibit new epitopes which interact with the anti-TNP antibodies. Such an interaction would allow anti-TNP antibodies to participate in a non-specific defence mechanism against viral infection.
authors.affiliation: Unité d'Immunocytochimie, CNRS URA 359, Institut Pasteur.
authors.forename: R
authors.initials: R
authors.lastname: Gonzalez
authors.forename: P
authors.initials: P
authors.lastname: Matsiota
authors.forename: C
authors.initials: C
authors.lastname: Torchy
authors.forename: P
authors.initials: P
authors.lastname: De Kinkelin
authors.forename: S
authors.initials: S
authors.lastname: Avrameas
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D007113:Immunity, Innate; D009578:Nitrobenzenes / Q000276:immunology*; D011487:Protein Conformation; D014301:Trinitrobenzenes / Q000276:immunology*; D014337:Trout; D014777:Virus Diseases / Q000276:immunology*
chemical_list: D000906:Antibodies; D009578:Nitrobenzenes; D014301:Trinitrobenzenes