Acid-base, plasma lactate and glucose changes in the rabbit following administration of Gaboon viper (Bitis gabonica) venom.
The acid-base and metabolic effects of Bitis gabonica venom administered intravenously to the anaesthetised rabbit were studied. Doubling doses of venom from 0.125 mg/kg to 1.0 mg/kg were used. Venom caused progressive and significant increases in plasma glucose and plasma lactate levels although oxygen consumption only became significantly lower after the fourth dose. Standard base excess (SBE) became significantly more negative after the third dose of venom and the fall in pH became significant at the same point. The results indicate that venom induces a metabolic acidosis in the rabbit and because the acidosis occurs in the absence of any fall in arterial PO2, it cannot be considered a consequence of impaired pulmonary ventilation. The reduction in oxygen uptake is likely to occur at a cellular level with a shift from aerobic to anaerobic metabolism hence the increase in plasma lactate levels. However, the magnitude of the acidosis is unlikely to be the principal cause of death under experimental conditions.
Gattullo
D
D
Dipartimento di Anatomia e Fisiologia Umana, Università degli Studi di Torino, Italy.
Hyslop
S
S
Marsh
N A
NA
Pagliaro
P
P
Vono
P
P
D000136:Acid-Base Equilibrium / Q000187:drug effects*; D000818:Animals; D001639:Bicarbonates / Q000097:blood; D001786:Blood Glucose / Q000378:metabolism*; D001794:Blood Pressure / Q000187:drug effects; D005260:Female; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000097:blood*; D008297:Male; D010100:Oxygen / Q000097:blood; D010101:Oxygen Consumption / Q000187:drug effects; D011817:Rabbits; D014757:Viper Venoms / Q000633:toxicity*
D001639:Bicarbonates; D001786:Blood Glucose; D007773:Lactates; D014757:Viper Venoms; D010100:Oxygen