The biased reptation model of DNA gel electrophoresis: mobility vs molecular size and gel concentration.
The biased reptation model provides a good framework for interpreting the results of continuous field DNA electrophoresis experiments performed in agarose gels. Here we discuss the main features of the mobility-molecular size and mobility-gel concentration diagrams as obtained from new extensive computer simulations of the model. Our aim is to suggest a global and coherent picture of this widely used yet poorly understood experimental technique, and to point out the areas where a systematic experimental study is still needed.
Slater
G W
GW
Noolandi
J
J
D003198:Computer Simulation; D004247:DNA / Q000032:analysis; D004586:Electrophoresis / Q000379:methods*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D008956:Models, Chemical; D008970:Molecular Weight
D004247:DNA