title: Flow cytometric analysis of protein kinase C in lymph node cells during the induction phase of contact hypersensitivity reaction.
abstract: On the fourth day after a single painting of 2,4,6-trinitro-chlorobenzene on the abdominal skin, inguinal lymph nodes were removed, and a single-cell suspension was prepared. The cells were analyzed flow cytometrically, using monoclonal anti-protein kinase C antibody. It was found that the number of lymph node cells in which protein kinase C was detected on the cell surface was significantly increased over that in non-treated mice (p less than 0.01). On the basis of our results and discussions in the literature, it is thought that protein kinase C is related to the initiation of the contact hypersensitivity reaction.
authors.forename: H
authors.initials: H
authors.lastname: Morita
authors.forename: M
authors.initials: M
authors.lastname: Yamagata
authors.forename: S
authors.initials: S
authors.lastname: Inohara
authors.forename: S
authors.initials: S
authors.lastname: Sagami
mesh_terms: D000818:Animals; D003877:Dermatitis, Contact / Q000201:enzymology* / Q000209:etiology; D005434:Flow Cytometry; D008198:Lymph Nodes / Q000201:enzymology; D051379:Mice; D008809:Mice, Inbred C3H; D010853:Picryl Chloride / Q000633:toxicity; D011493:Protein Kinase C / Q000378:metabolism*
chemical_list: D011493:Protein Kinase C; D010853:Picryl Chloride