[Effect of hemodialysate and its peptide fractions on stromal cells and heme synthesis in bone marrow culture and the activity of selected enzymes and GSH level of human erythrocytes. I. Effect on LDH activity in healthy persons and patients with terminal renal failure].
The purpose of the study was in evaluation of the effect of hemodialysate and its 3 peptide fractions on the lactic dehydrogenase (LDH, E.C.1.1.1.28) activity in erythrocytes in patients treated by repeated hemodialyses due to the terminal renal failure and in healthy subjects. It has been demonstrated that erythrocytes from hemodialysed patients show significantly diminished activity of LDH if compare with that in the healthy subjects. Activity of that enzyme in erythrocytes from healthy subjects after 3 hours lasting incubation with hemodialysate having concentration 675 and 450 micrograms/ml calculated against the protein content has significantly been lowered. Similarly, peptide fraction III in the incubative concentration 270 and 190 micrograms/ml induced significant inhibition of the enzyme activity. Both hemodialysate and its peptide fractions do not influenced the LDH activity in erythrocytes from patients treated by repeated hemodialyses.
Smoleński
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D001856:Bone Marrow Examination; D002478:Cells, Cultured; D003470:Culture Media; D015314:Dialysis Solutions / Q000494:pharmacology*; D004912:Erythrocytes / Q000201:enzymology*; D015312:Hemodialysis Solutions / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D066298:In Vitro Techniques; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000628:therapy; D007770:L-Lactate Dehydrogenase / Q000097:blood*; D010446:Peptide Fragments / Q000494:pharmacology*; D012016:Reference Values; D013727:Terminal Care
D003470:Culture Media; D015314:Dialysis Solutions; D015312:Hemodialysis Solutions; D010446:Peptide Fragments; D007770:L-Lactate Dehydrogenase
Wpływ hemodializatu i jego frakcji peptydowych na komórki podścieliska i synteze hemu w hodowli szpiku oraz na aktywność wybranych enzymów i stezenie GSH w krwinkach czerwonych człowieka. Cześć IV: Wpływ na aktywność LDH w erytrocytach ludzi zdrowych i chorych w schyłkowej niewydolności nerek.