Purification of human serum amyloid P component (SAP) by calcium affinity chromatography.
Serum amyloid P component (SAP) has been purified from human serum by means of immobilized metal ion affinity chromatography (IMAC). It was selectively concentrated on carboxymethylated aspartic acid agarose (CM-Asp-agarose) loaded with calcium and, employing very mild conditions, purified to electrophoretical and immunological homogeneity in a single step amounting to about 1900-fold purification. As a purification method our procedure thus compares well with bio-specific affinity chromatography.
Mantovaara
T
T
Institute of Biochemistry, University of Uppsala, Sweden.
Pertoft
H
H
Porath
J
J
D000596:Amino Acids / Q000032:analysis; D002118:Calcium; D002846:Chromatography, Affinity / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D011233:Precipitin Tests; D000683:Serum Amyloid P-Component / Q000302:isolation & purification*
D000596:Amino Acids; D000683:Serum Amyloid P-Component; D002118:Calcium