Cloning and sequencing of a cDNA encoding the precursor to the 24 kDa iron-sulfur protein of human mitochondrial NADH dehydrogenase.
1. We have isolated a cDNA encoding the 24 kDa subunit, an iron-sulfur protein, of mitochondrial NADH dehydrogenase from a human fibroblast cDNA library by colony hybridization using a rat 24 kDa subunit cDNA as a probe. 2. The presequence predicted from the human cDNA sequence is typical of precursors to mitochondrial proteins in a high content of basic residues and in the absence of acidic ones. 3. The mature form of the human 24 kDa subunit shows 95% homology with its rat counterpart. Five cysteine residues are conserved among human, rat and bovine; four of these are expected to be involved in the binding of a binuclear iron-sulfur cluster.
Toda
H
H
Department of Biomedical Chemistry, Faculty of Medicine, University of Nagoya, Japan.
Hosokawa
Y
Y
Nishikimi
M
M
Suzuki
H
H
Kato
K
K
Ozawa
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002417:Cattle; D002461:Cell Line, Transformed; D003001:Cloning, Molecular; D003579:Cytochrome Reductases / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D004792:Enzyme Precursors / Q000235:genetics*; D005347:Fibroblasts / Q000032:analysis; D006801:Humans; D007506:Iron-Sulfur Proteins / Q000235:genetics*; D008667:Metalloproteins / Q000235:genetics*; D008928:Mitochondria / Q000201:enzymology*; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics*; D051381:Rats; D012689:Sequence Homology, Nucleic Acid
D004792:Enzyme Precursors; D007506:Iron-Sulfur Proteins; D008667:Metalloproteins; D004247:DNA; D003579:Cytochrome Reductases; D009245:NADH Dehydrogenase