Sequence analysis of the tRNA(Tyr) and tRNA(Lys) genes and evidence for the transcription of a chloroplast-like tRNA(Met) in maize mitochondria.
The nucleotide sequences of three tRNA genes and their flanking regions from the maize mitochondrial genome is reported. These genes, which are located in the same region of the genome between the 14-kb inverted repeats, are transcribed in the mitochondria and code for tRNA(Lys) (anticodon UUU) tRNA(Met) (CAU) and tRNA(Tyr) (GUA). The very high homology shown by the tRNA(Met) gene with its chloroplast counterpart indicates that it probably originates from a chloroplast DNA insertion. The analysis of the upstream regions of these genes showed that the tRNA(Tyr) and the tRNA(Lys) genes possess the consensus sequence AAGAANRR, which could act as a promoter sequence in higher plant mitochondria.
Sangaré
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Institut de Biologie Moléculaire des Plantes, Strasbourg, France.
Lonsdale
D
D
Weil
J H
JH
Grienenberger
J M
JM
D001483:Base Sequence; D002736:Chloroplasts / Q000502:physiology; D008928:Mitochondria / Q000502:physiology*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D015347:RNA Probes; D012343:RNA, Transfer / Q000235:genetics*; D012357:RNA, Transfer, Lys / Q000235:genetics; D012358:RNA, Transfer, Met / Q000235:genetics; D012365:RNA, Transfer, Tyr / Q000235:genetics; D015183:Restriction Mapping; D014158:Transcription, Genetic; D003313:Zea mays / Q000235:genetics*
D015347:RNA Probes; D012357:RNA, Transfer, Lys; D012358:RNA, Transfer, Met; D012365:RNA, Transfer, Tyr; D012343:RNA, Transfer