A quantitative immunocytochemical study of the infiltrating lymphocytes in the spinal cord of guinea pigs with chronic relapsing experimental allergic encephalomyelitis.
The production and characterization of an anti-guinea pig B cell monoclonal antibody is described. Immunocytochemical techniques using this antibody and others recognizing a Pan T cell antigen and T cell subsets were employed to study frozen sections of spinal cord from guinea pigs with chronic relapsing experimental allergic encephalomyelitis. T and B cells were found in both perivascular lesions and the central nervous system parenchyma, with the major T cell infiltration occurring by the end of the acute phase of disease. The distribution of T cell subsets suggests a phenotypic selectivity in favour of the transport of CT6 (putative CD8)+ve cells across the blood-brain barrier.
Butter
C
C
Department of Pathology, Hunterian Institute, Royal College of Surgeons of England, London, U.K.
Healey
D G
DG
Baker
D
D
Turk
J L
JL
D000818:Animals; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000473:pathology; D001808:Blood Vessels / Q000473:pathology; D002908:Chronic Disease; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000473:pathology*; D006168:Guinea Pigs; D007150:Immunohistochemistry; D008214:Lymphocytes / Q000473:pathology*; D012008:Recurrence; D013116:Spinal Cord / Q000473:pathology*; D013601:T-Lymphocytes / Q000473:pathology
D000911:Antibodies, Monoclonal