title: Studies on the relationship between protein kinase C and differentiation of human promyelocytic leukemia cells induced by retinoic acid.
abstract: We studied the differentiation of acute promyelocytic leukemia (APL) cells in 14 patients with APL. After the induction by retinoic acid (RA) the mature cells rose to 60 +/- 11.8% compared to 0.7 +/- 1% of the control, while the promyelocytes declined to 8.7 +/- 6.4% (93.3 +/- 5.6% in the control group). Protein kinase C (PKC) activity was significantly increased to 149.3 +/- 156.2 pmol/mg per min compared to 47 +/- 40.9 of the control (p less than 0.01). In HL-60 cells, the activity of PKC increased also from 52.3 +/- 35 to 129.2 +/- 64.6 pmol/mg per min (n = 10, p less than 0.01) after the induction of differentiation with RA. If the leukemia cells were pretreated with a kind of PKC inhibitor such as trifluoperazine, the increase of PKC activity was inhibited, and the rate of nitroblue tetrazolium reduction decreased from 89.9 +/- 7.7% to 62 +/- 25% (n = 6, p less than 0.01) and the mature cells reduced from 63.1 +/- 11.7% to 19.7 +/- 12.2% (p less than 0.01). We presumed that the activity of PKC is closely related to the differentiation of human promyelocytic leukemia cells induced by all-trans-retinoic acid.
authors.affiliation: Hematology Department, Jin Ling Hospital, Nanjing, People's Republic of China.
authors.forename: X Z
authors.initials: XZ
authors.lastname: Wu
authors.forename: G Y
authors.initials: GY
authors.lastname: Shao
authors.forename: S
authors.initials: S
authors.lastname: Chen
authors.forename: X W
authors.initials: XW
authors.lastname: Wang
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Wang
mesh_terms: D000328:Adult; D002451:Cell Compartmentation / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D005260:Female; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000201:enzymology / Q000473:pathology*; D008297:Male; D011493:Protein Kinase C / Q000502:physiology*; D014212:Tretinoin / Q000494:pharmacology*; D014268:Trifluoperazine / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D014268:Trifluoperazine; D014212:Tretinoin; D011493:Protein Kinase C