Keratinocyte-specific transglutaminase of cultured human epidermal cells: relation to cross-linked envelope formation and terminal differentiation.
The predominant form of the cross-linking enzyme, transglutaminase, in cultured normal human epidermal keratinocytes, is found in cell particulate material and can be solubilized by nonionic detergent. It elutes as a single peak upon either anion-exchange or gel-filtration chromatography. Monoclonal antibodies raised to the particulate enzyme cross-react with one of two transglutaminases in the cell cytosol. The second cytosolic transglutaminase, which has distinct kinetic and physical properties from the first, does not cross-react and is not essential for formation of the keratinocyte cross-linked envelope in vitro. The anti-transglutaminase antibodies stain the more differentiated layers of epidermis in a pattern similar to that given by anti-involucrin antiserum. These observations support the hypothesis that the transglutaminase so identified is involved in cross-linked envelope formation in vivo.
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D000217:Acyltransferases / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002454:Cell Differentiation; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology / Q000648:ultrastructure; D002845:Chromatography; D003600:Cytosol / Q000201:enzymology; D004817:Epidermis / Q000201:enzymology* / Q000648:ultrastructure; D000939:Epitopes / Q000276:immunology; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007633:Keratins / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D011503:Transglutaminases
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007633:Keratins; D000217:Acyltransferases; D011503:Transglutaminases