Effects of theophylline on chromosomal breakage and sister-chromatid exchange.
Frequencies of both sister-chromatid exchange (SCE) and chromosomal breakage (CB) were studied in the lymphocytes of normal individuals (10 and 7 individuals respectively). The cells were exposed in vitro to 3 different concentrations of theophylline (1, 10 and 100 micrograms/ml). A significant concentration effect of the drug was demonstrated for both SCEs and CB. Utilizing a Dunnett's test for individual comparisons, the 10 and 100 micrograms/ml concentrations both demonstrated a significant elevation of SCEs and CB compared to the untreated control cultures. This study suggests that in vitro concentrations of theophylline equal to or greater than 10 micrograms/ml, corresponding to serum levels attained during therapy, increase the frequency of SCEs and chromosome breakage in human lymphocytes.
Day
P
P
Department of Obstetrics/Gynecology, University of Maryland, School of Medicine, Baltimore 21201.
Shalaby
Z
Z
Cohen
M M
MM
Wasserman
S S
SS
Schwartz
S
S
D002875:Chromosomes / Q000187:drug effects*; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D009153:Mutagens; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D013806:Theophylline / Q000009:adverse effects*
D009153:Mutagens; D013806:Theophylline