Platelet activating factor induced gastric damage in rats: participation of increased gastric vascular permeability.
Pharmacological factors involved in the injurious effect of platelet activating factor (PAF) on the gastric mucosa were studied in the acid (0.15 N HCI)-perfused stomachs of anesthetized rats. PAF infusion (100 ng/kg/min for 20 min, i.v.) induced distinct bleeding in the acid perfusate in proportion to mucosal lesions. Preceding the bleeding, a remarkable increase in mucosal vascular permeability was observed on dye-leakage assessment. Inhibition of the gastric bleeding and the dye leakage was achieved by pretreatment of the animals with CV-3988, a PAF antagonist, and hydrocortisone acetate, in contrast to the aggravation by caffeic acid. These results suggested the importance of mucosal vascular permeability in PAF-induced gastric mucosal lesions.
Hatakeyama
K
K
Department of Drug Evaluation and Toxicological Sciences, Faculty of Pharmaceutical Sciences, Chiba University, Japan.
Yano
S
S
Watanabe
K
K
D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects; D006471:Gastrointestinal Hemorrhage / Q000139:chemically induced / Q000503:physiopathology; D006851:Hydrochloric Acid / Q000494:pharmacology; D007275:Injections, Intravenous; D008297:Male; D010477:Perfusion; D010742:Phospholipid Ethers / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013270:Stomach / Q000098:blood supply / Q000187:drug effects* / Q000473:pathology
D010742:Phospholipid Ethers; D010972:Platelet Activating Factor; C037913:CV 3988; D006851:Hydrochloric Acid