One gene and one pseudogene for the cytokeratin endo A.
The recombinant cDNA RecXVI, which hybridizes to the endo A mRNA, detects two specific bands on a Southern blot of EcoRI-digested genomic mouse DNA. We have screened a mouse genomic library with this cDNA and isolated these two sequences. Endo A is encoded by a 7.5-kilobase gene and by a 1.6-kilobase pseudogene devoid of introns. A repetitive sequence belonging to the B2 family is located in the third intron of the gene. We have observed that transcription of B2 sequences and of the endo A gene are mutually exclusive.
Vasseur
M
M
Duprey
P
P
Brûlet
P
P
Jacob
F
F
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D004622:Embryo, Mammalian / Q000378:metabolism; D005786:Gene Expression Regulation; D005796:Genes; D007633:Keratins / Q000235:genetics*; D051379:Mice; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D007633:Keratins