Procoagulant activity of endocardial vegetations and blood monocytes in rabbits with Streptococcus sanguis endocarditis.
To investigate activation of the coagulation system in bacterial endocarditis, we determined the procoagulant activity of blood monocytes isolated from rabbits with Streptococcus sanguis-infected or sterile catheter-induced endocardial vegetations. This activity was determined directly after isolation from the peripheral blood and after stimulation in vitro by either endotoxin or by phagocytosis of S. sanguis. The procoagulant activity of the vegetations of these rabbits was also determined. The procoagulant activity of blood monocytes of rabbits with S. sanguis endocarditis was found to be similar to the activity of monocytes of rabbits with sterile vegetations, both at the time of isolation and after stimulation in vitro by exposure to endotoxin or phagocytosis of bacteria. The procoagulant activity of infected vegetations was significantly higher than that of sterile vegetations. We conclude that in bacterial endocarditis the coagulation system is activated locally at the site of the vegetation. Triggering probably occurs by thromboplastin generated by monocytes activated by phagocytosis of bacteria on the vegetational surface.
Buiting
A G
AG
Department of Infectious Diseases, University Hospital, Leiden, The Netherlands.
Thompson
J
J
van der Keur
D
D
Schmal-Bauer
W C
WC
Bertina
R M
RM
D000818:Animals; D001779:Blood Coagulation Factors / Q000032:analysis*; D004697:Endocarditis, Bacterial / Q000097:blood*; D004699:Endocardium / Q000032:analysis*; D005170:Factor X / Q000032:analysis; D008297:Male; D009000:Monocytes / Q000032:analysis*; D010587:Phagocytosis; D011516:Prothrombin / Q000032:analysis; D011817:Rabbits; D013290:Streptococcal Infections / Q000097:blood*; D013298:Streptococcus sanguis; D013925:Thromboplastin / Q000032:analysis*
D001779:Blood Coagulation Factors; C045071:leukocyte procoagulant activity; D011516:Prothrombin; D005170:Factor X; D013925:Thromboplastin