Automated conductimetric assay of human serum cholinesterase activity.
Serum cholinesterase activity was determined by conductimetry using samples in the microliter range. Butyrylcholine iodide was demonstrated to be a convenient substrate for the conductimetric assay. Validation of the microassay was made by using either purified enzyme or control serum. In the range of 0-60 U/l, a linear relationship was demonstrated. Correlation with a reference spectrophotometric method was obtained with a slope of 1.18. An explanation of this value is proposed, as different hydrolysis rates were obtained with human sera, depending on the substrate used (butyrylthio- or butyryl-choline ester).
Duffy
P
P
Laboratoire de Biochimie Analytique, UA CNRS244, Université de Lyon, Villeurbanne, France.
Wallach
J M
JM
D001322:Autoanalysis / Q000379:methods; D002091:Butyrylcholinesterase / Q000097:blood*; D002802:Cholinesterases / Q000097:blood*; D004563:Electrochemistry / Q000379:methods; D006801:Humans; D007700:Kinetics; D013053:Spectrophotometry / Q000379:methods; D013379:Substrate Specificity
D002091:Butyrylcholinesterase; D002802:Cholinesterases