Regulation of mouse ornithine decarboxylase activity by cell growth, serum and tetradecanoyl phorbol acetate is governed primarily by sequences within the coding region of the gene.
To determine the genetic elements required for modulation of ornithine decarboxylase (ODC) activity in response to cell growth or treatment with serum or with tetradecanoyl phorbol acetate, ODC-deficient cells were transfected with a series of recombinant DNAs encoding mouse ODC. All of the transfected cells expressing an intact mouse ODC protein displayed regulation of ODC activity, including those expressing a construct deprived of all ODC-specific sequence information except the protein-coding region. ODC mRNA changed much less than enzymatic activity. A mutation of the protein-coding region that converted ODC from an unstable to a stable intracellular protein attenuated the regulatory response. We conclude that post-transcriptional events associated with ODC degradation dominate the response to these stimuli.
van Daalen Wetters
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Department of Microbiology and Immunology, University of California, San Francisco 94143.
Brabant
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Coffino
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D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured; D003470:Culture Media; D004274:DNA, Recombinant / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005796:Genes / Q000187:drug effects; D007700:Kinetics; D008223:Lymphoma; D051379:Mice; D009955:Ornithine Decarboxylase / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014162:Transfection
D003470:Culture Media; D004274:DNA, Recombinant; D009955:Ornithine Decarboxylase; D013755:Tetradecanoylphorbol Acetate