title: Routine measurement of plasma catecholamines in clinical pharmacology by high-performance liquid chromatography with electrochemical detection.
abstract: A method for the simultaneous determination of epinephrine, norepinephrine and dopamine in human plasma is described, which combines the advantages of liquid-liquid extraction sample preparation, high-performance liquid chromatography on weak cation-exchange stationary phases and dual-electrode coulometric detection. The limits of quantification are less than 5 pg/ml (at a signal-to-noise ratio greater than 5) for each analyte. The influence of various experimental parameters (e.g., composition of the mobile phase, pretreatment of the assay buffer, components of the re-extraction system) on the performance of the assay is reported in detail. A number of applications are presented, which demonstrate the quality of the data obtained in terms of sensitivity, reproducibility and significance.
authors.affiliation: SK&F Institute for Applied Clinical Pharmacology, Göttingen, F.R.G.
authors.forename: I
authors.initials: I
authors.lastname: Meineke
authors.forename: E
authors.initials: E
authors.lastname: Stüwe
authors.forename: E M
authors.initials: EM
authors.lastname: Henne
authors.forename: G
authors.initials: G
authors.lastname: Rusteberg
authors.forename: E
authors.initials: E
authors.lastname: Brendel
authors.forename: C
authors.initials: C
authors.lastname: De Mey
mesh_terms: D002395:Catecholamines / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D006801:Humans
chemical_list: D002395:Catecholamines