title: Effect of spermidine on the conformation of bacteriophage MS2 RNA. Electron microscopy and computer modeling.
abstract: The structure of single-stranded RNA from the bacteriophage MS2 has been examined by electron microscopy in the presence of the polyamine spermidine. The molecules are found in two alternate conformations. The first of these can be characterized as a cruciform structure composed of three large loops approximately 500 to 700 nucleotides in size. The interior of the molecule has extensive base-paired regions which connect distant regions of the molecule; the farthest being 2500 nucleotides apart. In the second conformation, the molecules appear rod-like. Two of the large loops disappear, and these regions form, instead, extensive long-range helices. Computer modeling has been employed to explore the base-pairing potential of the sequence of bacteriophage MS2 RNA. Double-stranded regions identified by electron microscopy are shown to occur in local G + C-rich stretches of the RNA. Detailed models have been calculated for two regions of long-range contact. One of these includes the ribosome-binding site for the viral coat protein gene. The results are discussed in the context of the known role of RNA structure in the regulation of viral gene expression.
authors.forename: A B
authors.initials: AB
authors.lastname: Jacobson
authors.forename: H
authors.initials: H
authors.lastname: Kumar
authors.forename: M
authors.initials: M
authors.lastname: Zuker
mesh_terms: D001435:Bacteriophages / Q000032:analysis*; D001482:Base Composition; D001483:Base Sequence; D003201:Computers; D008854:Microscopy, Electron; D008954:Models, Biological; D009690:Nucleic Acid Conformation / Q000187:drug effects*; D012313:RNA; D012367:RNA, Viral; D013095:Spermidine / Q000494:pharmacology*
chemical_list: D012367:RNA, Viral; D012313:RNA; D013095:Spermidine