HPLC analysis of somatostatin peptides secreted by a rat pancreatic endocrine cell line (RINT3): stimulation studies.
A combination of anion exchange and reverse phase HPLC leading to the purification of a 15-kd proform of somatostatin from culture medium of the pancreatic tumoral endocrine RINT3 cell line is described. Elevation of extracellular calcium concentration causes a dose-dependent stimulation of somatostatin release; maximal stimulation (2.8-fold over basal) was reached with 5 mM Ca. Furthermore, 0.01 microM TPA induced a stimulation of about the same amplitude while forskolin had a low effect. These data suggest that secretion of somatostatin in this model can be regulated by the Ca/C protein-kinase pathway.
Lherisson
C
C
INSERM, U. 151, CHU Rangueil, Toulouse, France.
Estival
A
A
Pradayrol
L
L
Vaysse
N
N
D007516:Adenoma, Islet Cell / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D005576:Colforsin / Q000494:pharmacology; D003470:Culture Media; D007202:Indicators and Reagents; D010190:Pancreatic Neoplasms / Q000378:metabolism*; D010455:Peptides / Q000032:analysis* / Q000378:metabolism; D051381:Rats; D013004:Somatostatin / Q000032:analysis* / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D003470:Culture Media; D007202:Indicators and Reagents; D010455:Peptides; D005576:Colforsin; D013004:Somatostatin; D013755:Tetradecanoylphorbol Acetate