Molecular cloning and characterization of the complementary DNA coding for the B-chain of murine Clq.
cDNA clones coding for the B-chain of murine Clq were isolated from a mouse macrophage library. The characterized clones include the total coding region plus a leader sequence. High homology was found with human Clq B-chain in the coding region (81%). Northern blot analysis of total RNA from different tissues of Balb/c mice showed one band of approximately 1.2 kb. The highest signal was found in RNA preparations of thioglycolate-activated peritoneal macrophages. The probe also hybridized with mRNA from spleen, thymus and heart. Extremely weak signals were found in liver, kidney, lung and intestine tissues.
Petry
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Institute of Medical Microbiology, Johannes Gutenberg-University, Mainz, FRG.
Reid
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Loos
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D000818:Animals; D015152:Blotting, Northern; D003001:Cloning, Molecular; D015922:Complement C1q / Q000032:analysis / Q000235:genetics*; D004247:DNA / Q000032:analysis*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011401:Promoter Regions, Genetic; D021382:Protein Sorting Signals / Q000032:analysis; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis*; D012689:Sequence Homology, Nucleic Acid
D021382:Protein Sorting Signals; D015347:RNA Probes; D012333:RNA, Messenger; D015922:Complement C1q; D004247:DNA