Organ distribution of proteinase-resistant prion protein in humans and mice with Creutzfeldt-Jakob disease.
We attempted to clarify the organ distribution of human and murine proteinase-resistant prion protein (PrPCJD) in Creutzfeldt-Jakob disease (CJD), and to measure the concentration of PrPCJD, using a semi-quantitative Western blot analysis. Human PrPCJD was restricted to the central nervous system, whereas murine PrPCJD was present in the central nervous system and in the lymphoreticular system at the end stage of CJD. PrPCJD concentration in the central nervous system of mice was almost identical to that of humans. The minimum wet weight of an organ with a positive reaction was 0.3 mg for brain, 1 to 3 mg for spleen, 3 mg for spinal cord, 3 mg for lymph node, 10 mg for thymus and 10 to 30 mg for intestine of the CJD-infected mice. There were no immunoreactions in purified PrPCJD fractions from 300 mg of spleen, lymph node, liver or peripheral nervous systems of humans, nor in 300 mg of liver, lung or kidney of CJD-infected mice. Within the limits of our method, the distribution of murine PrPCJD differed from that of human PrPCJD. Antibodies on the Western blot membrane from murine spleen PrPCJD fractions stained the kuru plaques in the CJD-infected mouse brain. Therefore, PrPCJD in the murine spleen probably shares the epitopes of the antigen in the murine kuru plaques. Although the immunological detection of PrPCJD does have limits of sensitivity, PrPCJD concentrations did correlate with infectivity titres in scrapie-infected or CJD-infected mice.
Kitamoto
T
T
Department of Neuropathology, Kyushu University, Fukuoka, Japan.
Mohri
S
S
Tateishi
J
J
D000818:Animals; D015153:Blotting, Western; D002540:Cerebral Cortex / Q000032:analysis*; D007562:Creutzfeldt-Jakob Syndrome / Q000382:microbiology*; D006801:Humans; D007422:Intestines / Q000032:analysis; D008198:Lymph Nodes / Q000032:analysis; D051379:Mice; D018620:PrPSc Proteins; D013047:Specific Pathogen-Free Organisms; D013116:Spinal Cord / Q000032:analysis*; D013154:Spleen / Q000032:analysis*; D013950:Thymus Gland / Q000032:analysis; D014018:Tissue Distribution; D014764:Viral Proteins / Q000032:analysis*
D018620:PrPSc Proteins; D014764:Viral Proteins