Improved transmission electron microscopy technique for the study of cytologic material.
A modification of the technique of Coleman et al for the preparation of single cells in cytologic specimens for electron microscopy (EM) is described. By employing materials in the initial cytologic processing that are useful for EM, such as a paraformaldehyde-glutaraldehyde fixative, lactated Ringer's solution as a rinsing medium, glycerol as a mounting medium and cacodylate buffer for removal of coverslips, the use of alcohol fixatives and standard mounting media could be avoided. This preserved the cytoplasmic detail, which is usually degenerated in cells removed from cytologic specimens and processed for EM.
Hultgren
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Hidvegi
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D001707:Biopsy, Needle; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D002291:Carcinoma, Papillary; D002467:Cell Nucleus / Q000648:ultrastructure; D003584:Cytological Techniques; D003593:Cytoplasm / Q000648:ultrastructure; D005404:Fixatives; D005990:Glycerol; D006801:Humans; D007552:Isotonic Solutions; D008113:Liver Neoplasms / Q000473:pathology; D008854:Microscopy, Electron / Q000379:methods*; D009940:Organoids / Q000648:ultrastructure; D000077331:Ringer's Solution; D013194:Staining and Labeling; D013964:Thyroid Neoplasms / Q000648:ultrastructure
D005404:Fixatives; D007552:Isotonic Solutions; D000077331:Ringer's Solution; D005990:Glycerol