In vitro metabolism of the pro-carcinogen aflatoxin B1 by liver preparations of the calf, nurse shark and clearnose skate.
1. Liver postmitochondrial supernatant preparations of calf, clearnose skate, and nurse shark were able to metabolize the fungal toxin aflatoxin B1 to various metabolites. 2. Calf liver produced aflatoxin M1 and Q1 as the major chloroform soluble metabolites, with small amounts of aflatoxicol formed during incubation. 3. Liver preparations of the elasmobranchs, however, produced aflatoxicol as the major chloroform soluble metabolite with no other metabolite being detected. 4. The water soluble metabolite profiles for the three species were also quite different with the tris diol adduct being produced to a much greater extent in calf liver preparations. 5. Aflatoxicol production by the elasmobranch liver homogenates was reversible with the skate reconverting a large amount (30%) of aflatoxicol to AFB1. The nurse shark, however, appeared to convert a portion of aflatoxicol to an unknown metabolite more polar than AFB1. 6. Calf liver DNA bound approximately 3 x more 3H-AFB1 than shark liver DNA.
Bodine
A B
AB
Department of Dairy Science, Clemson University, South Carolina 29634-0363.
Luer
C A
CA
Gangjee
S A
SA
Walsh
C J
CJ
D016604:Aflatoxin B1; D000348:Aflatoxins / Q000378:metabolism*; D000818:Animals; D002273:Carcinogens / Q000378:metabolism*; D002417:Cattle / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D004555:Electric Fish / Q000378:metabolism*; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000378:metabolism; D012754:Sharks / Q000378:metabolism*; D012860:Skates, Fish / Q000378:metabolism*; D013045:Species Specificity
D000348:Aflatoxins; D002273:Carcinogens; D004247:DNA; D016604:Aflatoxin B1; D006899:Mixed Function Oxygenases