Treatment of human spermatozoa with an egg yolk medium can enhance the outcome of in vitro fertilization.
In at least 4 of 7 cases, fertilization of intact human oocytes was more successful when spermatozoa were pretreated with TEST yolk medium at 5 degrees C for 2 hours as compared with the standard treatment with Ham's F-10 only. Both pregnancies that were obtained after the transfer of the fertilized oocytes resulted from oocytes fertilized by TEST yolk-treated spermatozoa. No decrease in fertilization occurred in any of the cases after TEST yolk treatment. If these results hold true for a larger series of patients, it may be worthwhile for the standard IVF incubation system of spermatozoa to include TEST yolk.
Katayama
K P
KP
Advanced Institute of Fertility, Sinai Samaritan Medical Center, Milwaukee, Wisconsin.
Stehlik
E
E
Roesler
M
M
Jeyendran
R S
RS
Holmgren
W J
WJ
Zaneveld
L J
LJ
D003470:Culture Media; D004530:Egg Yolk; D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D013094:Spermatozoa / Q000187:drug effects*
D003470:Culture Media