Phospholipase D activation by the mitogens platelet-derived growth factor and 12-O-tetradecanoylphorbol 13-acetate in NIH-3T3 cells.
The effect of mitogens on phospholipase D activity was investigated in NIH-3T3 fibroblasts by measuring the accumulation of phosphatidylpropanol, produced by phospholipase D phosphatidyl transferase activity when 1-propanol acts as the phosphatidyl group acceptor. Platelet-derived growth factor (PDGF) and 12-O-tetradecanoylphorbol 13-acetate (TPA) stimulated phosphatidylpropanol production by the cells. The dose-response relationships for activation of phospholipase D and stimulation of thymidine incorporation by PDGF and TPA were comparable. The possibility that activation of phospholipase D is utilized by mitogens as a trans-membrane pathway for signalling cell growth is discussed.
Ben-Av
P
P
Department of Hormone Research, Weizmann Institute of Science, Rehovot, Israel.
Liscovitch
M
M
D000433:1-Propanol / Q000378:metabolism; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D051379:Mice; D008934:Mitogens; D010712:Phosphatidic Acids / Q000378:metabolism / Q000494:pharmacology; D010739:Phospholipase D / Q000378:metabolism*; D010740:Phospholipases / Q000378:metabolism*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D020005:Propanols; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D008934:Mitogens; D010712:Phosphatidic Acids; D010982:Platelet-Derived Growth Factor; D020005:Propanols; D000433:1-Propanol; D010740:Phospholipases; D010739:Phospholipase D; D013755:Tetradecanoylphorbol Acetate