title: Change in small intestinal brush border membranes of rats following methotrexate administration.
abstract: Change in small intestinal brush border membranes of rats following methotrexate administration was monitored by the fluorescence spectra and polarization of the cationic fluorescent probe, safranin 0. Total protein content of brush border membranes of treated rats was less than that of control rats, whereas no significant difference in total sialic acid content of brush border membranes was observed between them. Thus, an apparent increase in the electronegative charge of brush border membrane vesicles per unit of membrane protein following methotrexate administration may possibly cause the change in fluorescence spectra and polarization of safranin 0.
authors.affiliation: Department of Biopharmaceutics, Tokyo College of Pharmacy, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Takeuchi
authors.forename: Y
authors.initials: Y
authors.lastname: Kosakai
authors.forename: K
authors.initials: K
authors.lastname: Tsurui
authors.forename: K
authors.initials: K
authors.lastname: Hasegawa
authors.forename: T
authors.initials: T
authors.lastname: Horie
authors.forename: S
authors.initials: S
authors.lastname: Awazu
mesh_terms: D000818:Animals; D007421:Intestine, Small / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D008727:Methotrexate / Q000633:toxicity*; D008871:Microvilli / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: D008727:Methotrexate