Effects of in vivo heat treatment on lampbrush chromosome structure in amphibian oocytes.
When Pleurodeles (Amphibian, Urodele) females were subjected to high temperatures (32-35 degrees C) for varying periods of time (45 min to 7 days), lampbrush chromosome structure underwent striking modifications. These changes included a numerical reduction in normal loops and progressive disorganization of RNP matrices of various loops. The degree of such disorganization was a function of the intensity and duration of the stress. These modifications were completely reversible when females or oocytes were returned to a normal breeding temperature (20 degrees C). Results are discussed in comparison with previous studies on morphological changes induced by heat shock in lampbrush chromosomes carried out in vitro.
Rodriguez-Martin
M L
ML
Centre de Biologie Cellulaire (CNRS), Ivry sur Seine, France.
Herberts
C
C
Moreau
N
N
Angelier
N
N
D000818:Animals; D002875:Chromosomes / Q000648:ultrastructure*; D005260:Female; D006358:Hot Temperature; D066298:In Vitro Techniques; D009865:Oocytes / Q000166:cytology* / Q000502:physiology; D010999:Pleurodeles / Q000502:physiology*; D012016:Reference Values; D012455:Salamandridae / Q000502:physiology*