title: Characterization of the terminal cisternae and longitudinal tubules of sarcoplasmic reticulum from malignant hyperpyrexia susceptible porcine skeletal muscle.
abstract: 1. The sarcoplasmic reticulum (SR) from malignant hyperpyrexia susceptible (MHS) and control porcine skeletal muscle was separated into vesicular fractions enriched in the membrane elements of the terminal cisternae and longitudinal tubules. 2. The two membrane preparations were highly purified and had distinctive features which were associated with their origins in the SR membraneous network. 3. Calsequestrin and calcium were enriched in the terminal cisternae fraction (HSR), in comparison to longitudinal tubule preparations (LSR). 4. The HSR membrane also had a greater total capacity to store Ca2+ and Ca2+ release was more rapid than from LSR preparations. 5. No distinction could be made between the membrane morphology, Ca2+ -fluxes or Ca2+ -dependent ATPase activities, associated with these functionally distinct regions of MHS and control preparations.
authors.affiliation: Division of Biochemistry and Molecular Biology, John Curtin School of Medical Research, Australian National University, Canberra, ACT.
authors.forename: P S
authors.initials: PS
authors.lastname: Foster
authors.forename: M D
authors.initials: MD
authors.lastname: White
authors.forename: M A
authors.initials: MA
authors.lastname: Denborough
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D008305:Malignant Hyperthermia / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D008854:Microscopy, Electron; D012519:Sarcoplasmic Reticulum / Q000378:metabolism / Q000648:ultrastructure*; D013552:Swine; D013553:Swine Diseases / Q000378:metabolism / Q000473:pathology*
chemical_list: D002118:Calcium