Diagnosis of Corynebacterium pseudotuberculosis infections in sheep, using an enzyme-linked immunosorbent assay.
Lambs infected with Corynebacterium pseudotuberculosis (ovis) by injecting suspensions of live bacteria into the wool-free area of the axilla developed antibody against cell wall antigens and antitoxin, as measured by the enzyme-linked immunosorbent assay. The toxin was a better antigen for measuring infection than was the cell wall antigen. The enzyme-linked immunosorbent assay appeared to be as sensitive as the antihemolysin inhibition test for detecting antitoxin and was easier to perform.
Maki
L R
LR
Shen
S H
SH
Bergstrom
R C
RC
Stetzenbach
L D
LD
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D000942:Antigens, Bacterial / Q000276:immunology; D000992:Antitoxins / Q000032:analysis; D001427:Bacterial Toxins / Q000276:immunology; D003352:Corynebacterium / Q000276:immunology; D003354:Corynebacterium Infections / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D005260:Female; D008199:Lymphadenitis / Q000662:veterinary*; D008297:Male; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis*; D013010:Sonication
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D000992:Antitoxins; D001427:Bacterial Toxins; D000939:Epitopes