title: Major structural alterations of the c-sis gene are not observed in a series of tumors of the human central nervous system.
abstract: Expression of the c-sis oncogene, the gene encoding the B chain of platelet-derived growth factor (PDGF), may be related to initiation and/or progression of glial cell tumorigenesis by PDGF-mediated autocrine growth stimulation. As the mechanism for activation of expression of the c-sis gene in gliomas is not known, we searched for possible structural alterations of c-sis DNA in these tumors. Genomic Southern blots of DNA from 7 different cultured human glioblastoma cell lines and 15 different solid human brain tumors revealed no significant change in either the gross structure or the copy number of the c-sis gene in tumor cells vs. control cells. Activation of glioma c-sis gene expression is therefore not the result of a gross rearrangement or amplification of the c-sis gene. Expression of c-sis mRNA was detected in all of 12 different solid human brain tumors, 11 of which were of glial cell origin. However, in tissue adjacent to 5 different tumors, approximately the same level of c-sis mRNA was seen.
authors.affiliation: Department of Biochemistry, Case Western Reserve University Cleveland, OH 44106.
authors.forename: R D
authors.initials: RD
authors.lastname: Press
authors.forename: A
authors.initials: A
authors.lastname: Misra
authors.forename: D
authors.initials: D
authors.lastname: Samols
authors.forename: D A
authors.initials: DA
authors.lastname: Goldthwait
authors.forename: T B
authors.initials: TB
authors.lastname: Mapstone
mesh_terms: D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D002648:Child; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005910:Glioma / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D020575:Oncogene Proteins v-sis; D010982:Platelet-Derived Growth Factor / Q000235:genetics* / Q000378:metabolism; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics*
chemical_list: D004273:DNA, Neoplasm; D020575:Oncogene Proteins v-sis; D010982:Platelet-Derived Growth Factor; D015682:Retroviridae Proteins, Oncogenic