[Specificity of enzymes of biosynthesis of Salmonella anatum O-antigen for polyprenyl derivatives of different chain length and saturation].
Polyprenyl phosphates of different structure were prepared and their ability to serve as sugar acceptors in the biosynthesis of O-specific polysaccharides of Salmonella anatum was investigated. It was demonstrated that C30-C80-polyprenyl phosphates with unsaturated alpha-isoprene unit were as active as natural acceptor (undecaprenyl phosphate) in this enzymic system. C15- and C100-polyprenyl phosphates of this series were less effective in O-antigen repeating unit formation. Citronellyl and dolichyl phosphates (derivatives of C10- and C105-polyprenols, respectively, with saturated alpha-isoprene unit) were poor substrates. For polymerization of repeating units, the polyprenol chain-length is of utmost importance: its shortening results in a marked drop in the efficiency of respective compounds as substrates.
Kalinchuk
N A
NA
Danilov
L L
LL
Druzhinina
T N
TN
Shibaev
V N
VN
Kochetkov
N K
NK
D000942:Antigens, Bacterial; D005700:Galactosyltransferases; D019081:O Antigens; D011105:Polyisoprenyl Phosphate Sugars / Q000138:chemical synthesis*; D011106:Polyisoprenyl Phosphates / Q000138:chemical synthesis; D011108:Polymers; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D012475:Salmonella / Q000276:immunology*; D013379:Substrate Specificity
D000942:Antigens, Bacterial; D019081:O Antigens; D011105:Polyisoprenyl Phosphate Sugars; D011106:Polyisoprenyl Phosphates; D011108:Polymers; D011135:Polysaccharides, Bacterial; D005700:Galactosyltransferases
Spetsifichnost' fermentov biosinteza O-antigena Salmonella anatum k proizvodnym poliprenolov raznoĭ dliny tsepi i nasyshchennosti.