Recombinant interleukin 2 rapidly augments human natural killer cell activity.
Recombinant human interleukin 2 (r-IL-2) rapidly stimulated human natural killer cell activity in vitro. Augmentation of NK activity occurred within 1 hr of preincubation with r-IL-2. Responsive killer cells were typical NK cells as shown by cell fractionation procedures. These included Percoll density gradient separation and depletion of OKT3+ T cells by an indirect rosetting method. Analysis with a panel of polyclonal and monoclonal antibodies against alpha and gamma interferon revealed that this early enhancement of NK activity by r-IL-2 was independent of the production of both types of interferon.
Kabelitz
D
D
Kirchner
H
H
Armerding
D
D
Wagner
H
H
D000906:Antibodies / Q000276:immunology; D004274:DNA, Recombinant; D006801:Humans; D066298:In Vitro Techniques; D007372:Interferons / Q000096:biosynthesis / Q000276:immunology; D007376:Interleukin-2 / Q000235:genetics / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology*; D011971:Receptors, Immunologic / Q000276:immunology; D015375:Receptors, Interleukin-2
D000906:Antibodies; D004274:DNA, Recombinant; D007376:Interleukin-2; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2; D007372:Interferons