Effect of different procedures of semen preparation on antibody-coated spermatozoa and immunological infertility.
To assess whether procedures of semen preparation can reduce the proportion of antibody-coated spermatozoa, semen samples with positive direct mixed antiglobulin reaction (MAR) were washed in media supplemented with 10% or 50% fetal cord serum (FCS). Washing reduced the MAR to a negative level, but the MAR was identical to that in the native semen when spermatozoa were resuspended in serum-free medium. Donor spermatozoa, recovered after swim-up in media supplemented with 10% or 50% FCS or after passage through a column with 7.5% human serum albumin (HSA), were incubated in serum samples with both agglutinating and cytotoxic antisperm antibodies. Cytotoxic activity was significantly reduced against sperm filtered over the albumin column.
Hinting
A
A
Department of Internal Medicine, State University Hospital, Ghent, Belgium.
Vermeulen
L
L
Goethals
I
I
Dhont
M
M
Comhaire
F
F
D000373:Agglutinins / Q000032:analysis; D001323:Autoantibodies / Q000032:analysis*; D002469:Cell Separation / Q000379:methods; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007248:Infertility, Male / Q000628:therapy*; D008297:Male; D012661:Semen / Q000032:analysis*; D013094:Spermatozoa / Q000166:cytology / Q000276:immunology*
D000373:Agglutinins; D001323:Autoantibodies