title: Morbillivirus infection of seals (Phoca vitulina) during the 1988 epidemic in the Bay of Heligoland. I. Mode, frequency and significance of cultural virus isolation and neutralizing antibody detection.
abstract: From 16 (14%) out of 112 dead or euthanized seals originating from wildlife and seal orphanages phocine morbillivirus was isolated. The majority of viral isolates in cell cultures was obtained from lung homogenates of 15 out of 71 free-ranging seals (21%). The virus was isolated by longterm cultivation in roller cultures of seal kidney cells. The phocine morbillivirus was detected by typical cytopathogenic alteration and by peroxidase-linked antibody (PLA) assay, respectively. A neutralization test based on PLA was used for antibody detection in seals using a canine distemper virus (CDV) strain and in parallel one of the phocine morbillivirus isolates. All sera tested were proven to contain neutralizing antibodies of higher titres against the latter virus than against the CDV strain. Several seals furnished morbillivirus isolates and at the same time exhibited neutralizing antibodies of low to medium titres. No viral isolates were obtained from the majority of sick animals with moderate to high neutralizing titres (greater than 1/1,000). The significance of these findings is discussed in relation to the cause of the mass mortality amongst seals observed in 1988 in the Bay of Heligoland.
authors.forename: B
authors.initials: B
authors.lastname: Liess
authors.forename: H R
authors.initials: HR
authors.lastname: Frey
authors.forename: A
authors.initials: A
authors.lastname: Zaghawa
authors.forename: M
authors.initials: M
authors.lastname: Stede
mesh_terms: D000818:Animals; D010872:Caniformia; D004196:Disease Outbreaks / Q000662:veterinary*; D005860:Germany, West; D008459:Measles virus / Q000302:isolation & purification*; D009500:Neutralization Tests; D010253:Respirovirus Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D046048:Seals, Earless