title: Delayed methylation and the matrix bound DNA methylase.
abstract: It is shown that the methylation of DNA that occurs in isolated nuclei is "delayed methylation". This methylation is not reduced in nuclei which have been pretreated with 0.2M NaCl to extract the soluble methylase suggesting that this methylation is the product of a firmly bound matrix associated DNA methylase. Evidence is provided that, like the methylase, the DNA substrate is associated with the nuclear matrix.
authors.forename: T
authors.initials: T
authors.lastname: Davis
authors.forename: D
authors.initials: D
authors.lastname: Kirk
authors.forename: A
authors.initials: A
authors.lastname: Rinaldi
authors.forename: R H
authors.initials: RH
authors.lastname: Burdon
authors.forename: R L
authors.initials: RL
authors.lastname: Adams
mesh_terms: D000818:Animals; D001374:Azacitidine / Q000031:analogs & derivatives / Q000378:metabolism; D001665:Binding Sites; D002467:Cell Nucleus / Q000201:enzymology; D004247:DNA / Q000378:metabolism*; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000378:metabolism*; D000077209:Decitabine; D066298:In Vitro Techniques; D007739:L Cells / Q000378:metabolism; D008745:Methylation; D008780:Methyltransferases / Q000378:metabolism*; D051379:Mice; D013997:Time Factors
chemical_list: D000077209:Decitabine; D004247:DNA; D008780:Methyltransferases; D004248:DNA (Cytosine-5-)-Methyltransferases; D001374:Azacitidine