Heat inactivation of serum potentiates anti-cardiolipin antibody binding in ELISA.
Heat treatment of sera at 56 degrees C for 30 min results in positive ELISA reactions for anti-cardiolipin antibody (aCL) in sera that had undetectable or low levels of aCL before heat inactivation. The positive, potentiated reactivity of the heated sera in the aCL ELISA could be inhibited with the cardiolipin antigen and was abolished by prior IgG depletion using staphylococcal protein A. The heat-potentiating effect of aCL binding in ELISA was evident in both normal human sera and clinical sera including sera from patients with systemic lupus erythematosus and syphilis.
Cheng
H M
HM
Department of Physiology, University of Malaya, Kuala Lumpur.
Ngeow
Y F
YF
Sam
C K
CK
D002308:Cardiolipins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006358:Hot Temperature; D006801:Humans; D007136:Immunoglobulins / Q000302:isolation & purification*; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D013587:Syphilis / Q000276:immunology
D002308:Cardiolipins; D007136:Immunoglobulins