[Radioimmunoassay of vasoactive intestinal peptide immunoreactivity in guinea pig respiratory tract].
Although vasoactive intestinal peptide (VIP) has been suggested to be the neurotransmitter of non-adrenergic and non-cholinergic (NANC) inhibitory nerves, its physiological functions and movements in the airway are obscure. In this study, VIP immunoreactivity in the respiratory tract from guinea pigs was measured as a preliminary experiment to elucidate its functions. VIP immunoreactivity was measured by radioimmunoassay. The rate of VIP disappearance during extraction was 52.5 +/- 17.4 (mean +/- SD)%. The dose-response curve of tissue extract almost paralleled the standard curve of authentic VIP. VIP immunoreactivity of tracheas was 939.9 +/- 262.1 pg/g wet weight and that of extrapulmonary bronchi was 858.0 +/- 241.1 pg/g wet weight. Although VIP immunoreactivity of lung extracts was not detectable in 14 samples out of 23, the value of 9 samples was 111.7 +/- 61.5 pg/g wet weight. These results suggest that there may be more VIP immunoreactivity present in tracheas and extrapulmonary bronchi than in lungs.
Ishizuka
T
T
Kurosawa
M
M
D000818:Animals; D006168:Guinea Pigs; D008297:Male; D011863:Radioimmunoassay; D012137:Respiratory System / Q000032:analysis*; D014660:Vasoactive Intestinal Peptide / Q000032:analysis* / Q000276:immunology
D014660:Vasoactive Intestinal Peptide