[Experimental study on myocardial preservation with perfluorochemical].
The effect of perfluorochemical as cardioplegic solution was studied with isolated canine hearts. They were divided into two groups as follows each consisting of ten, and cardioplegia was made every 30 minutes during 3 hours of ischemia. Group I: The solution was oxygenated to PaO2 of 542 +/- 67 mmHg (mean +/- SD). Group II: The solution was deoxygenated to PaO2 of 55 +/- 12 mmHg. Both temperature were 20 degrees C. After 3 hours cardiac arrest, the hearts were fixed to the perfusion unit filled up with the diluted blood. Then hemodynamic and biochemical variables were measured every 30 minutes. There were some significant differences between the groups. Hemodynamic indices especially negative LV max dp/dt were recovered excellently in Group I but not so much in Group II. Negative LV max dp/dt, which was the distinction of the diastole, showed significant difference more than positive LV max dp/dt, which was the distinction of the systole. It was considered that under the same condition, negative LV max dp/dt reflected not only compliance but also preparatory ability of the left ventricle, and it could be one of the important indices evaluating cardiac function. As regarding metabolism, delivery of oxygen with cardioplegic solution was good for the aerobic metabolism also after reperfusion, and in these circumstances, catecholamine was available effectively to the hearts. The conclusion is as follows. It is important for myocardial preservation to suppress the anaerobic metabolism and to keep the circumstances in which catecholamine was available effectively. And oxygenated PFC is good to preserve myocardium and useful as cardioplegic solution.
Tsukamoto
S
S
D000818:Animals; D002314:Cardioplegic Solutions; D004285:Dogs; D005466:Fluorocarbons / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects / Q000502:physiology; D006439:Hemodynamics / Q000187:drug effects; D066298:In Vitro Techniques; D009206:Myocardium / Q000378:metabolism; D009926:Organ Preservation; D010100:Oxygen / Q000494:pharmacology
D002314:Cardioplegic Solutions; D005466:Fluorocarbons; C008807:perfluorotributylamine; D010100:Oxygen