Isolation of a cDNA clone and localization of the human glutathione S-transferase 3 genes to chromosome bands 11q13 and 12q13-14.
A partial cDNA clone of the glutathione S-transferase 3 gene (GST3) was obtained by screening a lambda gt11 human lung cDNA library with antiserum to human lung GST3. The sequence of this cDNA showed two base differences from the coding sequence of a GST3 cDNA isolated from a human placental cDNA library. Hybridization of the cloned GST3 cDNA to human chromosomes resulted in a primary peak of grains over band 11q13, a localization predicted by prior experiments. An unexpected strong secondary peak of grains was obtained over bands 12q13 and 12q14, indicating that there is a GST3-like gene on the long arm of chromosome 12 in man.
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Division of Clinical Sciences, John Curtin School of Medical Research, Australian National University, Canberra.
Webb
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Coggan
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D000595:Amino Acid Sequence; D001483:Base Sequence; D002871:Chromosome Banding; D002880:Chromosomes, Human, Pair 11; D002881:Chromosomes, Human, Pair 12; D004247:DNA / Q000302:isolation & purification*; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D007527:Isoenzymes / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization
D007527:Isoenzymes; D004247:DNA; D005982:Glutathione Transferase