Evolution of antibody structure and effector functions: comparative hemolytic activities of monomeric and tetrameric IgM from rainbow trout, Salmo gairdnerii.
Monomeric and tetrameric IgM anti-haptin antibodies isolated from the sera of rainbow trout (S. gairdnerii) by immunoaffinity chromatography were compared both immunochemically and with regard to their functional abilities to lyse haptenated trout erythrocytes in the presence of trout complement. The two populations had similar binding affinities for hapten and apparently identical L chains, but differed in H chain peptide maps and immunoreactivity with rabbit anti-trout H chain serum. These differences could not be attributed to J-chain. The abilities of the two antibody subpopulations to activate C to lyse haptenated trout erythrocytes also differed dramatically. Such functional differences are not simply explained by the greater avidity of the tetrameric form since preliminary studies show that the monomeric form of trout IgM activates C via an alternative pathway mechanism while the tetrameric form activates both classical and alternative pathway mechanisms. Results suggest divergent evolution of antibody structures involved in the familiar effector functions (C activation, transport, etc.).
Elcombe
B M
BM
Chang
R J
RJ
Taves
C J
CJ
Winkelhake
J L
JL
D000818:Animals; D000917:Antibody Formation; D000936:Antigen-Antibody Complex / Q000032:analysis; D005075:Biological Evolution; D002846:Chromatography, Affinity; D000939:Epitopes / Q000032:analysis*; D006241:Haptens; D006461:Hemolysis; D005779:Immunodiffusion; D007075:Immunoglobulin M / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D046911:Macromolecular Substances; D010446:Peptide Fragments / Q000032:analysis; D012487:Salmonidae / Q000276:immunology*; D014337:Trout / Q000276:immunology*
D000936:Antigen-Antibody Complex; D000939:Epitopes; D006241:Haptens; D007075:Immunoglobulin M; D046911:Macromolecular Substances; D010446:Peptide Fragments