Effects of rapid glucose infusion on in vivo and in vitro free fatty acid re-esterification by adipose tissue of fasted obese subjects.
Adipose tissue lipolysis and fatty acid re-esterification were examined simultaneously in vivo and in vitro in fasted human subjects receiving a constant intravenous infusion of 14C palmitate and large intravenous infusions of glucose and insulin. Under these circumstances, the plasma [FFA] declines rapidly while the specific activity of plasma FFA increases. Plasma [glycerol] remains stable or declines slowly. These in vivo changes in FFA and glycerol metabolism appear to reflect increased adipose tissue re-esterification of FFA, without an attendant decline in rate of lipolysis. However, in vitro rates of re-esterification in gluteal adipose tissue did not change sufficiently to account for the in vivo phenomena. We conclude that: (1) re-esterification in adipose tissue is an important mechanism for the decline in [FFA] induced by glucose infusion in fasted man, but in vitro studies of adipose tissue from a gluteal site do not explain the changes in plasma free fatty acid levels; (2) our data suggest that control of the net mixture of metabolic fuels leaving the adipocyte may be under the influence of pericellular blood flow in addition to ambient humoral factors or intrinsic characteristics of the adipocyte.
Leibel
R L
RL
Laboratory of Human Behaviour and Metabolism, Rockefeller University, New York, NY 10021.
Forse
R A
RA
Hirsch
J
J
D000328:Adult; D005215:Fasting / Q000378:metabolism*; D005230:Fatty Acids, Nonesterified / Q000378:metabolism*; D005260:Female; D005947:Glucose / Q000008:administration & dosage / Q000378:metabolism*; D006801:Humans; D007261:Infusions, Intra-Arterial; D007262:Infusions, Intravenous; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000378:metabolism*
D005230:Fatty Acids, Nonesterified; D005947:Glucose