Determination of homocysteine in plasma by ion-exchange chromatography.
A rapid ion-exchange chromatographic method for determination of total homocysteine in plasma is presented. The method has a coefficient of variation of approximately 5%. A comparison with a slower method previously used in our laboratory showed a high correlation (r = 0.993). The risk of oxidation of homocysteine during the chromatography and the necessary level of dithiotreithol to prevent oxidation are also established.
Andersson
A
A
Department of Clinical Chemistry, University Hospital, Lund, Sweden.
Brattström
L
L
Isaksson
A
A
Israelsson
B
B
Hultberg
B
B
D002852:Chromatography, Ion Exchange / Q000379:methods; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D008715:Methionine / Q000097:blood; D010084:Oxidation-Reduction
D006710:Homocysteine; D008715:Methionine