title: Mitogenic activities for skin fibroblasts in the sera from untreated scleroderma patients at the early stage.
abstract: Mitogenic activities for adult skin fibroblasts in 17 sera from untreated patients with scleroderma at the early stage were studied. Mitogenic activities in 10% test sera were measured by the [3H]-thymidine incorporation assay using confluent fibroblasts; there was no significant difference between scleroderma-affected and normal controls. Growth-stimulatory activities for subconfluent fibroblasts in 10% test sera were measured by increased cell numbers; again, there was no significant difference between scleroderma-affected and normal controls. There have been contradictory reports on growth-stimulatory activities in scleroderma sera; our data do not suggest the presence of a specific mitogen in scleroderma sera.
authors.affiliation: Department of Dermatology, Faculty of Medicine, University of Tokyo, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Igarashi
authors.forename: K
authors.initials: K
authors.lastname: Takehara
authors.forename: Y
authors.initials: Y
authors.lastname: Soma
authors.forename: K
authors.initials: K
authors.lastname: Kikuchi
authors.forename: Y
authors.initials: Y
authors.lastname: Ishibashi
mesh_terms: D000328:Adult; D000368:Aged; D002455:Cell Division; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000473:pathology; D006133:Growth Substances / Q000097:blood; D006801:Humans; D008875:Middle Aged; D008934:Mitogens / Q000097:blood*; D012595:Scleroderma, Systemic / Q000097:blood* / Q000473:pathology; D012867:Skin / Q000473:pathology*
chemical_list: D006133:Growth Substances; D008934:Mitogens