The induction of Friend erythroleukaemia differentiation is markedly affected by expression of a transfected c-myb cDNA.
A minigene containing a cDNA encoding the normal mouse p80c-myb protein under strong promoter control was used to transfect Friend erythroleukaemia cells. Expression of the transfected gene was found to result in elevated levels of p80c-myb which were not subject to rapid down-modulation by inducers of Friend cell differentiation as was the product of the endogenous c-myb gene. Continued synthesis of p80c-myb was found to be associated with a marked decrease in differentiation of Friend cells and we concluded that normal down-regulation of c-myb expression may be a necessary event in differentiation of these cells.
McMahon
J
J
Imperial Cancer Research Fund Laboratories, St Bartholomew's Hospital, London.
Howe
K M
KM
Watson
R J
RJ
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D018076:DNA, Complementary / Q000378:metabolism; D005622:Friend murine leukemia virus / Q000235:genetics; D015870:Gene Expression; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D009857:Oncogenes; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics; D020598:Proto-Oncogene Proteins c-myb; D011519:Proto-Oncogenes; D014162:Transfection
D018076:DNA, Complementary; D011518:Proto-Oncogene Proteins; D020598:Proto-Oncogene Proteins c-myb