Fish oil prevents effect of high cholesterol diet on active intestinal transport of galactose.
We tested the hypothesis that diets containing fish oils prevent the effects of a high cholesterol diet on the morphology and nutrient uptake of the intestine. Isocaloric semisynthetic diets were supplemented with beef tallow or fish oil containing low or high amounts of cholesterol and were fed to growing female Wistar rats for 14 days, after which the in vitro jejunal and ileal uptake of glucose, galactose, long-chain fatty acids, and cholesterol was determined. Feeding cholesterol with beef tallow was associated with a 12% decrease in the jejunal mucosal surface area. Feeding fish oil decreased jejunal mucosal surface area by 24%, as compared with the beef tallow diet, but the reduction was increased to 42% when fish oil and cholesterol were fed together. Ileal surface area was unaffected by varying the major source of dietary lipid, or by adding cholesterol. Despite the effect of fish oil on the mucosal surface area, the jejunal and ileal uptake of saturated as well as unsaturated long-chain fatty acids and cholesterol was similar in the four diet groups. Cholesterol supplementation enhanced the jejunal uptake of high concentrations of galactose only when fed with beef tallow, i.e., feeding fish oil prevented the enhancing effect of cholesterol on galactose uptake observed when beef tallow was fed. Thus, (i) a fish oil diet prevents the enhancing effect of cholesterol on jejunal active transport of galactose, an effect not explained by the reduction in jejunal mucosal surface area observed with the fish oil diet; (ii) these dietary manipulations result in a clear dissociation of the morphological from the transport adaptation of the intestine; and (iii) substitution of fish oil for beef tallow as the major source of lipid in the diet prevents the influence of cholesterol on the active intestinal transport of galactose.
Thomson
A B
AB
Nutrition and Metabolism Research Group, University of Alberta, Edmonton, Canada.
Keelan
M
M
Garg
M L
ML
Clandinin
M T
MT
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002791:Cholesterol, Dietary / Q000378:metabolism*; D004435:Eating; D005260:Female; D005395:Fish Oils / Q000378:metabolism*; D005690:Galactose / Q000378:metabolism* / Q000493:pharmacokinetics; D005947:Glucose / Q000378:metabolism / Q000493:pharmacokinetics; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism; D007583:Jejunum / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D002791:Cholesterol, Dietary; D005395:Fish Oils; D005947:Glucose; D005690:Galactose