Radio-immunoassay of somatostatin from isolated rat pancreatic islets.
Certain aspects of radio-immunoassay of somatostatin from isolated rat pancreatic islets are described. Somatostatin-14, and not somatostatin-28, is secreted from isolated rat pancreatic islets. Less somatostatin secretion is measured per islet owing to purity of tracer in the radio-immunoassay. Theophylline apparently cross-reacts with somatostatin in the assay described, and this has to be taken into consideration when studying somatostatin release induced by theophylline in isolated islets.
Vonen
B
B
Department of Medicine, University Hospital of Tromsø, Norway.
Florholmen
J
J
Giaever
A K
AK
Burhol
P
P
D000818:Animals; D005947:Glucose / Q000494:pharmacology; D007515:Islets of Langerhans / Q000378:metabolism*; D011786:Quality Control; D011863:Radioimmunoassay; D051381:Rats; D013004:Somatostatin / Q000032:analysis* / Q000378:metabolism; D053561:Somatostatin-28; D013806:Theophylline / Q000032:analysis / Q000494:pharmacology
D013004:Somatostatin; D053561:Somatostatin-28; D013806:Theophylline; D005947:Glucose