Effects of diphenylamine-2-carboxylate on sodium and chloride transport across bovine tracheal epithelium: studies in monolayers and in the native tissue.
1. The short-circuit current (I0) across monolayers of bovine tracheal epithelial cells is the sum of Na+ absorption and Cl- secretion. 2. Diphenylamine-2-carboxylate (DPC), added to the mucosal side of the native tissue or monolayers induced a rapid, dose-dependent and fully reversible reduction in I0, which reached zero with 3 mM DPC. 3. The blocking effect of DPC was examined during incubation (1) in standard, (2) in Cl(-)-free and (3) in Na+-free solution. Dose response curves revealed that the IC50 was not altered following ion substitution: it was approximately 0.7 mM DPC. 4. Thus, in bovine tracheal epithelium, DPC was an effective blocking agent of both Na+ and Cl- transport.
Durand
J
J
Institute of Physiology, University of Fribourg, Switzerland.
D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002034:Bumetanide / Q000494:pharmacology; D002417:Cattle; D002712:Chlorides / Q000378:metabolism*; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D066298:In Vitro Techniques; D012964:Sodium / Q000378:metabolism*; D014132:Trachea / Q000187:drug effects / Q000378:metabolism*; D062367:ortho-Aminobenzoates / Q000494:pharmacology*
D002712:Chlorides; D062367:ortho-Aminobenzoates; D002034:Bumetanide; D000584:Amiloride; C001209:fenamic acid; D012964:Sodium