[Use of phase diagrams in crystallization of oligonucleotide duplexes. II. Setting of the crystallized samples].
Oligonucleotide crystallization technique based on the method of phase diagrams is described in detail with (pGpT)3.(pApC)3 hexamer as an example. The key point of the technique consists of dividing the multiparameter crystallization space into a set of regions, each of which corresponds to the precipitation of a duplex in complex with a certain number of counterions.
Tereshko
V A
VA
Malinina
L V
LV
D003460:Crystallization; D009690:Nucleic Acid Conformation; D009692:Nucleic Acid Heteroduplexes; D009841:Oligonucleotides
D009692:Nucleic Acid Heteroduplexes; D009841:Oligonucleotides
Ispol'zovanie fazovykh diagramm pri kristallizatsii oligonukleotidnykhdupleksov. II. Postanovka kristallizatsionnykh prob.