title: [The acridine orange test. A new parameter in assessing the fertilizing capacity of spermatozoa].
abstract: Simultaneously with in vitro fertilization (IVF), the acridine orange (AO) test was performed on 51 patients according to the method of Tejada et al. This test allows a differentiation of spermatozoa with intact (double-stranded) and denatured (single-sanded) DNA. A significant relationship was demonstrated between the percentage of green-fluorescing (intact) sperm and the results of IVF. For both, the groups with (n = 40) and without (n = 11) fertilization of human eggs, a positive correlation was found between AO-test and sperm motility, and AO-test and normal sperm morphology.
authors.affiliation: Abteilung Frauenheilkunde und Geburtshilfe, Universität Ulm.
authors.forename: K
authors.initials: K
authors.lastname: Sterzik
authors.forename: B
authors.initials: B
authors.lastname: Rosenbusch
authors.forename: V
authors.initials: V
authors.lastname: Sasse
authors.forename: E
authors.initials: E
authors.lastname: Wild
authors.forename: W
authors.initials: W
authors.lastname: Hütter
authors.forename: A
authors.initials: A
authors.lastname: Wolf
mesh_terms: D000165:Acridine Orange; D000328:Adult; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007248:Infertility, Male / Q000175:diagnosis / Q000503:physiopathology; D008297:Male; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D013081:Sperm Motility / Q000502:physiology*; D013084:Sperm-Ovum Interactions / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology
chemical_list: D000165:Acridine Orange
vernacular_title: Der Acridin-Orange-Test. Ein neuer Parameter zur Beurteilung der Befruchtungsfähigkeit von Spermatozoen.