title: [Restriction-modification systems of type II in Shigella strains].
abstract: Two restriction-modification systems specified by two plasmids are discovered in the clinical species of Shigella. The plasmids are designated pKMR114 and pKMR115. Both are of 60.800 bp and belong to the IncN incompatibility group. The EcoRI, EcoRV, HindIII restriction patterns of both plasmid DNAs are identical. As shown by efficiency of plating of bacteriophage lambda vir on the strains harbouring plasmids encoding EcoRI, EcoRII, EcoRIII, EcoRIV, EcoRV systems and plasmids studied, the discovered plasmids control synthesis of EcoRII specificity enzymes. The main distinctive feature of the pKMR114 is the ability to decrease efficiency of plating of bacteriophage T4 having glycolised DNA.
authors.forename: L i
authors.initials: Li
authors.lastname: Solodukhina
authors.forename: M Sh
authors.initials: MSh
authors.lastname: Manuvakhova
authors.forename: A I
authors.initials: AI
authors.lastname: Korotiaev
mesh_terms: D004269:DNA, Bacterial / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics*; D006868:Hydrolysis; D008970:Molecular Weight; D010957:Plasmids; D012760:Shigella / Q000201:enzymology* / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; C046545:CCWGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific
vernacular_title: Sistemy restriktsii-modifikatsii vtorogo tipa shtammov roda Shigella.