Value of aceto-orcein squash preparations in the cytogenetic study of solid tumours.
The value of aceto-orcein squash preparations as a routine initial step in the cytogenetic study of solid tumours is pointed out. This simple procedure allows a rapid evaluation to be made of various characteristics of the tumour, including the ploidy level, as well as a definitive assessment of nuclear phenomena including the incidence of X-chromatin bodies. Evaluation of the latter in normal as well as tumour cells led to the discovery of unsuspected congenital sex chromosome anomalies in two patients in this laboratory.
Atkin
N B
NB
Department of Cancer Research, Mount Vernon Hospital, Northwood, UK.
Baker
M C
MC
D000230:Adenocarcinoma / Q000235:genetics; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics; D002295:Carcinoma, Transitional Cell / Q000235:genetics; D002869:Chromosome Aberrations; D003110:Colonic Neoplasms / Q000235:genetics; D004396:Coloring Agents; D005260:Female; D006801:Humans; D007621:Karyotyping; D009369:Neoplasms / Q000235:genetics*; D010051:Ovarian Neoplasms / Q000235:genetics; D010078:Oxazines; D001749:Urinary Bladder Neoplasms / Q000235:genetics; D014594:Uterine Neoplasms / Q000235:genetics; D014675:Vegetables
D004396:Coloring Agents; D010078:Oxazines; C018437:aceto-orcein