The effect of 5-azacytidine and its analogues on blast cell renewal in acute myeloblastic leukemia.
Blast cells from patients with acute myeloblastic leukemia were exposed to 5-azacytidine (5-aza) and its analogues 5-aza 2'-deoxycytidine (5-aza-dr) and 6-azacytidine (6-aza). Simple negative exponential survival curves were obtained for the three drugs, but the sensitivity varied; 5-aza-dr was most toxic, 6-aza was least toxic, and 5-aza was intermediate. Colonies surviving drug exposure were replated; 5-aza and 5-aza-dr were found to increase secondary plating efficiency, whereas 6-aza was inactive. The findings provide indirect evidence for a role for DNA methylation in the regulation of blast cell self-renewal.
Motoji
T
T
Hoang
T
T
Tritchler
D
D
McCulloch
E A
EA
D001374:Azacitidine / Q000031:analogs & derivatives / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects*; D003470:Culture Media; D000077209:Decitabine; D004305:Dose-Response Relationship, Drug; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000097:blood* / Q000473:pathology; D014411:Neoplastic Stem Cells / Q000187:drug effects*; D010641:Phenotype; D013234:Stem Cells / Q000187:drug effects*
D003470:Culture Media; D000077209:Decitabine; D001374:Azacitidine; C040881:6-azacytidine