Determination of dopamine, norepinephrine, serotonin and their major metabolic products in rat brain by reverse-phase ion-pair high performance liquid chromatography with electrochemical detection.
A method is described for the separation and quantitation of catecholamines, serotonin, and their major metabolites with use of reverse-phase, ion-pair liquid chromatography with electrochemical detection. This method employs columns packed with a microparticulate C-18 resin, octyl sodium sulfate as the ion-pairing agent, and isocratic elution with a citrate-phosphate buffer containing methanol. Conditions are described for the separation of norepinephrine, normetanephrine, 3-methoxy-4-hydroxyphenylglycol, epinephrine, metanephrine, dopamine, 3,4-dihydroxyphenylacetic acid, homovanillic acid, serotonin, and 5-hydroxyindole-3-acetic acid and for their quantitation in extracts of rat brain tissue.
Kotake
C
C
Heffner
T
T
Vosmer
G
G
Seiden
L
L
D015102:3,4-Dihydroxyphenylacetic Acid / Q000032:analysis; D000818:Animals; D001923:Brain Chemistry; D002421:Caudate Nucleus / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004298:Dopamine / Q000032:analysis*; D004563:Electrochemistry; D006719:Homovanillic Acid / Q000032:analysis; D006897:Hydroxyindoleacetic Acid / Q000032:analysis; D007031:Hypothalamus / Q000032:analysis; D008734:Methoxyhydroxyphenylglycol / Q000032:analysis; D009638:Norepinephrine / Q000032:analysis*; D009647:Normetanephrine / Q000032:analysis; D011699:Putamen / Q000032:analysis; D051381:Rats; D012701:Serotonin / Q000032:analysis*
D009647:Normetanephrine; D015102:3,4-Dihydroxyphenylacetic Acid; D012701:Serotonin; D008734:Methoxyhydroxyphenylglycol; D006897:Hydroxyindoleacetic Acid; D004298:Dopamine; D009638:Norepinephrine; D006719:Homovanillic Acid