title: beta-D-N-acetylglucosaminidase, a new cytochemical marker of human lymphocyte subpopulations.
abstract: beta-D-N-acetylglucosaminidase staining characteristics of rosetted or non-rosetted normal and malignant lymphoid cells were compared with those observed after nonspecific esterase and acid phosphatase staining. With the three cytochemical techniques a similar staining pattern was observed in T cells (E-rosettes), their subpopulations T mu and T gamma, B cells and the non-T, non-B cells, as well as in the T cell populations defined with the monoclonal antibodies OKT3,4 and 8. T mu cells mostly displayed a "dot-like" reaction, T gamma and the non-T, non-B cells a "fine to heavy granular" reaction, while most B cells were negative. OKT4 and OKT8 positive lymphocytes showed for the larger part a dot-like staining pattern, however, the frequency of cells with a granular pattern was distinctly higher in the OKT8, than in the OKT4 positive cells. E(+)mIg(-) and E(-)mIg(-) A.L.L. blasts stained either with a dot-like or granular pattern or failed to react when stained cytochemically for beta-D-N-acetylglucosaminidase, nonspecific esterase and acid phosphatase activity. Only in a few instances a discrepancy was observed between the types of staining for esterase and acid phosphatase on one hand and those for beta-D-N-acetylglucosaminidase on the other.
authors.forename: E
authors.initials: E
authors.lastname: Orye
authors.forename: Y
authors.initials: Y
authors.lastname: Benoit
authors.forename: L
authors.initials: L
authors.lastname: Roesbeke
authors.forename: M
authors.initials: M
authors.lastname: De Wilde
authors.forename: M
authors.initials: M
authors.lastname: Van Yper
mesh_terms: D000118:Acetylglucosaminidase / Q000032:analysis*; D000135:Acid Phosphatase / Q000032:analysis; D000328:Adult; D001402:B-Lymphocytes / Q000145:classification; D002648:Child; D002675:Child, Preschool; D005260:Female; D006596:Hexosaminidases / Q000032:analysis*; D006651:Histocytochemistry; D006801:Humans; D007945:Leukemia, Lymphoid / Q000473:pathology; D008214:Lymphocytes / Q000145:classification*; D008297:Male; D008875:Middle Aged; D009283:Naphthol AS D Esterase / Q000032:analysis; D012397:Rosette Formation; D013194:Staining and Labeling / Q000379:methods*; D013601:T-Lymphocytes / Q000145:classification
chemical_list: D009283:Naphthol AS D Esterase; D000135:Acid Phosphatase; D006596:Hexosaminidases; D000118:Acetylglucosaminidase