[Determination of the substrate specificity of Bpu101 restrictase with an unusual recognition segment].
A new enzyme Bpu10I was isolated from Bacillus pumilus. This enzyme is not an isoschizomer of any known restriction endonucleases. The search of possible recognition sequences was carried out in sequences ABCNiDEF (i = 0.6) on substrate DNA lambda CI857, T7, pBR322. The recognition sequence and cleavage sites of restriction endonuclease Bpu10I have been determined as CCTNAGC. GGANTCG
Degtiarev
S Kh
SKh
Zhilkin
P A
PA
Prikhod'ko
G G
GG
Rechkunova
N I
NI
Repin
V E
VE
D001407:Bacillus / Q000201:enzymology*; D001483:Base Sequence; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D008969:Molecular Sequence Data; D013379:Substrate Specificity
C061882:endodeoxyribonuclease Bpu10I; D015252:Deoxyribonucleases, Type II Site-Specific
Opredelenie substratnoĭ spetsifichnosti restriktazy BPu101 s neobychnym uchastkom uznavaniia.