title: Citrate synthase: an immunochemical investigation of interspecies diversity.
abstract: Rabbit antibodies have been raised to pig heart citrate synthase. Using purified IgG, competitive enzyme-linked immunoassays and assays of citrate synthase activity indicate the presence of antibodies to a number of antigenic sites on the enzyme, only some of which are essential for catalytic activity. From a comparison of citrate synthases from prokaryotic and eukaryotic organisms, the degree of interaction between antibody and enzyme was in the order: pig heart greater than pigeon breast greater than Bacillus megaterium greater than Escherichia coli. These findings are discussed in terms of the known interspecies diversity of the enzyme.
authors.forename: A M
authors.initials: AM
authors.lastname: Pullen
authors.forename: N
authors.initials: N
authors.lastname: Budgen
authors.forename: M J
authors.initials: MJ
authors.lastname: Danson
authors.forename: D W
authors.initials: DW
authors.lastname: Hough
mesh_terms: D000818:Animals; D001407:Bacillus / Q000201:enzymology; D002950:Citrate (si)-Synthase / Q000276:immunology*; D010856:Columbidae; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D004926:Escherichia coli / Q000201:enzymology; D009132:Muscles / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D007652:Oxo-Acid-Lyases / Q000276:immunology*; D011817:Rabbits; D013045:Species Specificity; D013552:Swine
chemical_list: D000939:Epitopes; D002950:Citrate (si)-Synthase; D007652:Oxo-Acid-Lyases