RFLP analysis of DLA class I genes in the dog.
Human major histocompatibility complex (HLA) cDNA probes for class I genes HLA-A,B and HLA-E were used to analyze the Restriction Fragment Length Polymorphism (RFLP) of the class I region of the canine major histocompatibility complex (DLA) in 40 dogs. The Southern blot analysis demonstrated that the dog genome contains at least eight class I genes, including the canine homologues of HLA-A,B and HLA-E genes. The DNA polymorphism detected by the HLA-B7 probe corresponded to the serologically defined DLA-A allelic series. Restriction fragments that correlated with the DLA-A2, -A7 and -A9 antigenic specificities were identified in PstI digests of genomic DNA. The RFLP analysis was particularly useful in genotyping dogs which were not clearly DLA-A typable by serology. This technique can be used as a supplement to serotyping and as a genotyping tool for DLA antigenic specificities for which specific antisera are not available.
Sarmiento
U M
UM
Fred Hutchinson Cancer Research Center, Seattle, Washington.
Storb
R F
RF
D000818:Animals; D015343:DNA Probes, HLA; D004285:Dogs / Q000276:immunology*; D015234:HLA-A Antigens / Q000235:genetics; D015235:HLA-B Antigens / Q000235:genetics; D015793:HLA-B7 Antigen / Q000235:genetics; D015395:Histocompatibility Antigens Class I / Q000235:genetics* / Q000276:immunology; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length
D015343:DNA Probes, HLA; D015234:HLA-A Antigens; D015235:HLA-B Antigens; D015793:HLA-B7 Antigen; D015395:Histocompatibility Antigens Class I