The effect of GTP hydrolysis and transpeptidation on the arrangement of aminoacyl-tRNA at the A-site of Escherichia coli 70 S ribosomes.
From the affinity labelling of 70 S ribosomes with a photoreactive derivative of Phe-tRNAPhe bearing an arylazido group on guanine residues, it has been found that different sets of ribosomal proteins are labelled in the course of three successive steps of EF-Tu-dependent binding of aminoacyl-tRNA derivative at the A-site. Proteins S5, S7, S8, S16, S17, L9, L14, L15 and L24 were labelled before GTP hydrolysis; proteins S5, S7, S9, S11, S14, S18, S19, S21, L9, L21 and L29--after GTP hydrolysis; proteins S2, S5, S7, S21, L11 and L23--after GTP hydrolysis and transpeptidation.
Vladimirov
S N
SN
Graifer
D M
DM
Karpova
G G
GG
Semenkov YuP
Makhno
V I
VI
Kirillov
S V
SV
D000345:Affinity Labels; D001386:Azides / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000378:metabolism*; D010444:Peptide Elongation Factor Tu; D010445:Peptide Elongation Factors / Q000494:pharmacology; D010777:Photochemistry; D012329:RNA, Bacterial / Q000378:metabolism*; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*
D000345:Affinity Labels; D001386:Azides; D010445:Peptide Elongation Factors; D012329:RNA, Bacterial; D012346:RNA, Transfer, Amino Acyl; D012269:Ribosomal Proteins; C038012:tRNA, azidophenylalanine-; D006160:Guanosine Triphosphate; D010444:Peptide Elongation Factor Tu