Cysteamine normalizes cerebral somatostatin level and binding in pentylenetetrazol-kindled rats.
Rats were kindled by intraperitoneal injection of pentylenetetrazol (PTZ) (30 mg/Kg) every 48 h. Once kindled, some of the animals received a single injection of cysteamine (200 mg/Kg). Somatostatin-like immunoreactivity (SLI) and 125 I-Tyr11-somatostatin binding were measured in the frontoparietal cortex and hippocampus of the two experimental groups and the control rats. After PTZ kindling the following was observed: 1) SLI content was increased in the two areas; 2) Somatostatin receptor affinity decreased in the frontoparietal cortex and was unaltered in the hippocampus; 3) The number of somatostatin receptors decreased in the hippocampus and was unaltered in the frontoparietal cortex. Cysteamine, an agent which depletes brain somatostatin and suppresses kindled seizures in PTZ-treated rats, reversed the altered SLI levels and binding in these rats.
Perez-Oso
E
E
Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Alcalá de Henares, Madrid, Spain.
Lopez-Ruiz
M P
MP
Gonzalez-Guijarro
L
L
Arilla
E
E
D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D003543:Cysteamine / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D007696:Kindling, Neurologic / Q000187:drug effects*; D007700:Kinetics; D010296:Parietal Lobe / Q000187:drug effects / Q000378:metabolism; D010433:Pentylenetetrazole / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains; D017981:Receptors, Neurotransmitter / Q000378:metabolism; D017481:Receptors, Somatostatin; D012016:Reference Values; D013004:Somatostatin / Q000378:metabolism*
D017981:Receptors, Neurotransmitter; D017481:Receptors, Somatostatin; D013004:Somatostatin; D003543:Cysteamine; D010433:Pentylenetetrazole