title: Separation of collagens by capillary zone electrophoresis.
abstract: Collagen (types I, II, V, IX and XI) constituting polypeptide chains and their polymers and cyanogen bromide-cleaved peptides of collagen type I and type III were investigated by means of capillary zone electrophoresis. Separations were effected in 2.5 mM sodium tetraborate buffer in less than 15 min. A 50 cm x 0.1 mm I.D. fused-silica capillary was used. The separations were run at 18 kV per capillary. The results of the separation were monitored at 220 nm with an on-tube detection system. Using the Offord equation, relative retention times of cyanogen bromide cleavage fragments were plotted against M(2-3)/Z, where M is the molecular mass of a polypeptide and Z its valency. A linear relationship was observed. Collagen alpha-chains and their polymers were also satisfactorily resolved.
authors.affiliation: Institute of Physiology, Czechoslovak Academy of Science, Prague.
authors.forename: Z
authors.initials: Z
authors.lastname: Deyl
authors.forename: V
authors.initials: V
authors.lastname: Rohlicek
authors.forename: M
authors.initials: M
authors.lastname: Adam
mesh_terms: D003094:Collagen / Q000302:isolation & purification*; D003488:Cyanogen Bromide; D004586:Electrophoresis / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D007202:Indicators and Reagents
chemical_list: D007202:Indicators and Reagents; D003094:Collagen; D003488:Cyanogen Bromide