title: A 64,000 dalton matrix protein of human cytomegalovirus induces in vitro immune responses similar to those of whole viral antigen.
abstract: Human cytomegalovirus contains approximately 30 to 35 structural polypeptides. Although antibodies to several of these proteins are made during natural infection, their relationship to T cell recognition of this virus and subsequent control of infection is poorly understood. We have purified one of these proteins (HCMVgp64) that is found in abundance in infected cell lysates in order to delineate the relationship of single viral proteins to the immune response caused by the virus. HCMVgp64 induced T cell reactivity only in individuals with serologic evidence of past infection. In addition, HCMVgp64 elicited similar in vitro immune reactions as the whole virus including T cell proliferation, interleukin 2 production, and receptor expression as well as interferon production. These studies suggest that single proteins of HCMV such as HCMVgp64 are capable of inducing T cell responses and may be important in the development of immune reactivity to HCMV.
authors.forename: S J
authors.initials: SJ
authors.lastname: Forman
authors.forename: J A
authors.initials: JA
authors.lastname: Zaia
authors.forename: B R
authors.initials: BR
authors.lastname: Clark
authors.forename: C L
authors.initials: CL
authors.lastname: Wright
authors.forename: B J
authors.initials: BJ
authors.lastname: Mills
authors.forename: R
authors.initials: R
authors.lastname: Pottathil
authors.forename: B C
authors.initials: BC
authors.lastname: Racklin
authors.forename: M T
authors.initials: MT
authors.lastname: Gallagher
authors.forename: K
authors.initials: K
authors.lastname: Welte
authors.forename: K G
authors.initials: KG
authors.lastname: Blume
mesh_terms: D000956:Antigens, Viral / Q000276:immunology*; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000276:immunology; D006801:Humans; D007372:Interferons / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis / Q000378:metabolism; D008213:Lymphocyte Activation; D008970:Molecular Weight; D011971:Receptors, Immunologic / Q000096:biosynthesis; D015375:Receptors, Interleukin-2; D013601:T-Lymphocytes / Q000276:immunology*; D014763:Viral Matrix Proteins; D014764:Viral Proteins / Q000276:immunology* / Q000502:physiology
chemical_list: D000956:Antigens, Viral; D007376:Interleukin-2; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2; D014763:Viral Matrix Proteins; D014764:Viral Proteins; D007372:Interferons