Transcription factor MBF-I interacts with metal regulatory elements of higher eucaryotic metallothionein genes.
Metallothionein (MT) gene promoters in higher eucaryotes contain multiple metal regulatory elements (MREs) that are responsible for the metal induction of MT gene transcription. We identified and purified to near homogeneity a 74-kilodalton mouse nuclear protein that specifically binds to certain MRE sequences. This protein, MBF-I, was purified employing as an affinity reagent a trout MRE that is shown to be functional in mouse cells but which lacks the G+C-rich and SP1-like sequences found in many mammalian MT gene promoters. Using point-mutated MREs, we showed that there is a strong correlation between DNA binding in vitro and MT gene regulation in vivo, suggesting a direct role of MBF-I in MT gene transcription. We also showed that MBF-I can induce MT gene transcription in vitro in a mouse extract and that this stimulation requires zinc.
Imbert
J
J
Laboratory of Biochemistry, National Cancer Institute, Bethesda, Maryland 20892.
Zafarullah
M
M
Culotta
V C
VC
Gedamu
L
L
Hamer
D
D
D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D005796:Genes; D005809:Genes, Regulator; D007739:L Cells / Q000378:metabolism; D008668:Metallothionein / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D009695:Nucleic Acid Renaturation; D015345:Oligonucleotide Probes; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D014157:Transcription Factors / Q000302:isolation & purification / Q000378:metabolism*; D014158:Transcription, Genetic; D014162:Transfection
D015345:Oligonucleotide Probes; D014157:Transcription Factors; D008668:Metallothionein