Effects of human recombinant interleukin 5 and 3 on the differentiation of cord blood-derived eosinophils and basophils.
When mononuclear cells from umbilical-cord blood were cultured for 3 weeks, low concentrations of interleukin 3 supported the preferential growth of basophils, with eosinophils comprising a smaller proportion. These basophils contained 0.15-0.3 microgram histamine per 10(6) cells, and released histamine by the IgE-dependent and -independent stimuli. Interleukin 5 increased the number and proportion of eosinophils in a dose-dependent manner without affecting the proliferation of other cell types in the interleukin 3-supplemented cultures. These cultured eosinophils could be activated by platelet-activating factor.
Ebisawa
M
M
Saito
H
H
Reason
D C
DC
Ohno
K
K
Sudo
T
T
Kurihara
K
K
Nagakura
T
T
Iikura
Y
Y
D001491:Basophils / Q000166:cytology*; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D004804:Eosinophils / Q000166:cytology*; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D007377:Interleukin-3 / Q000494:pharmacology*; D015848:Interleukin-5 / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology
D007377:Interleukin-3; D015848:Interleukin-5; D011994:Recombinant Proteins