Plasmid analysis and antimicrobial susceptibilities of Peptostreptococcus species.
There are no published methods on plasmid isolation from Peptostreptococcus spp., therefore two methods of plasmid isolation from this genera were analysed: the boiling and alkaline-SDS methods. Plasmid DNA was not recovered by the boiling method, however, with the alkaline-SDS method, cryptic plasmid DNA was detected in two P. asaccharolyticus and one P. magnus strains. To achieve optimum lysis, Peptostreptococcus cells were treated with lysozyme (2 mg/ml) for 15 min. at 37 degrees C followed by proteinase K (0.2 mg/ml) for 1 h at 37 degrees C. In addition we report, the occurrence of clindamycin or metronidazole-resistant peptostreptococci, but these phenotypes were not correlated with plasmid carriage.
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Department of Biology, University of Ottawa, Canada.
Dillon
J A
JA
D004269:DNA, Bacterial / Q000032:analysis; D004352:Drug Resistance, Microbial / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D009113:Muramidase; D010462:Peptostreptococcus / Q000235:genetics*; D011815:R Factors
D004269:DNA, Bacterial; D009113:Muramidase