Mechanism of action of colchicine. VI: Effect of colchicine on generation of leukotriene B4 by human polymorphonuclear leukocytes.
Three microtubule-disruptive agents (colchicine, oncodazole, and vinblastine) are shown to suppress ionophore A23187-induced generation of LTB4 in a dose-related manner. The slopes of the dose-response regression lines were similar for the three drugs. Trimethyl-colchicinic acid has no effect on LTB4 generation. The suppressive effect of colchicine (1 x 10(-6) M) can be blocked completely by taxol (1 x 10(-6) M) which promotes and stabilizes microtubule assembly. The data suggest that colchicine suppresses the generation of LTB4 via its effect on microtubules.
Ouyang
Y
Y
Department of Medicine, School of Medicine, University of California, Los Angeles, CA 90024.
Wang
W
W
Bhuta
S
S
Chang
Y H
YH
D000470:Alkaloids / Q000494:pharmacology; D000001:Calcimycin / Q000037:antagonists & inhibitors / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D003078:Colchicine / Q000494:pharmacology*; D006801:Humans; D007975:Leukotriene B4 / Q000032:analysis / Q000096:biosynthesis*; D008854:Microscopy, Electron; D008870:Microtubules / Q000187:drug effects* / Q000648:ultrastructure; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D015739:Nocodazole / Q000494:pharmacology; D017239:Paclitaxel; D014527:Uric Acid / Q000494:pharmacology; D014747:Vinblastine / Q000494:pharmacology
D000470:Alkaloids; D007975:Leukotriene B4; D014527:Uric Acid; D000001:Calcimycin; D014747:Vinblastine; D017239:Paclitaxel; D015739:Nocodazole; D003078:Colchicine