Characterization of activated peritoneal cells inhibiting the take of transplantable murine tumours.
We studied the properties of activated peritoneal cells (PC) inhibiting the take of SP4 spontaneous adenocarcinoma and Lewis lung carcinoma in syngeneic mice. Treatment of the poly I:C activated PC from Balb/c mice suppressing the take of SP4 tumour with anti-asialo GM1 antibody and complement before transfer did not affect their tumour-inhibitory potential. PC from Balb/c nude mice treated with poly I:C also inhibited the take of SP4 tumour. Spleen cells from untreated or poly I:C treated Balb/c and Balb/c nude mice, however, did not inhibit the take of SP4 adenocarcinoma. Treatment of peritoneal cells activated by a combination of poly I:C, indomethacin and Syncumar (referred to as "combined treatment") with anti-asialo GM1 antibody and complement could not, or could only partly abolish their tumour-inhibitory potential. The cells mediating the suppression of the take of Lewis lung tumour proved to be Thy-1,2+/-, Lyt-1-, Lyt 2.2- cells. We conclude that the activated peritoneal cells inhibiting the take of SP4 adenocarcinoma and Lewis lung tumour are different from NK cells, NC cells and LAK cells and represent a distinct antitumoural effector cell population.
Minárovits
J
J
National Institute of Hygiene, Microbiological Research Group, Budapest, Hungary.
Karczag
E
E
Földes
I
I
D000074:Acenocoumarol / Q000494:pharmacology; D000230:Adenocarcinoma / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D002277:Carcinoma / Q000276:immunology*; D003165:Complement System Proteins / Q000494:pharmacology; D005260:Female; D005677:G(M1) Ganglioside; D006028:Glycosphingolipids / Q000276:immunology; D007213:Indomethacin / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D010529:Peritoneal Cavity / Q000166:cytology*; D011070:Poly I-C / Q000494:pharmacology
D000906:Antibodies; D006028:Glycosphingolipids; D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside; D003165:Complement System Proteins; D000074:Acenocoumarol; D011070:Poly I-C; D007213:Indomethacin