High resolution R banding in amniotic fluid cells using the BrdU-Hoechst 33258-Giemsa (RBG) technique.
While standard Giemsa banding is generally adequate for amniotic fluid chromosome analysis, small deletions, duplications, or translocation breakpoints involving G-negative bands may be difficult to appreciate. We report a method for producing high resolution R banding in human amniotic fluid cultures using the BrdU-Hoechst 33258-Giemsa (RBG) technique. RBG banding can be useful in confirming and precisely defining structural abnormalities in amniotic fluid cultures.
Cheung
S W
SW
Crane
J P
JP
Burgess
A C
AC
D000653:Amniotic Fluid / Q000166:cytology; D001399:Azure Stains; D006690:Bisbenzimidazole; D001973:Bromodeoxyuridine; D002869:Chromosome Aberrations / Q000175:diagnosis*; D002871:Chromosome Banding / Q000379:methods*; D025063:Chromosome Disorders; D005260:Female; D006801:Humans; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*
D001399:Azure Stains; D001973:Bromodeoxyuridine; D006690:Bisbenzimidazole