Changes in the intracellular calcium ion concentration in the gastric mucosa in a rat ischemia-reperfusion model.
To investigate the role of the intracellular calcium ion in the development of acute gastric mucosal lesions, phosphorylase a activity was measured as an index of the intracellular calcium ion concentration ([Ca2+]i), using Lowry's method, in the rat ischemia-reperfusion model. [Ca2+]i increased significantly at the end of the ischemic state without acute mucosal lesions (AGML). After reinfusion, [Ca2+]i showed a slight increase and AGML developed. Continual intravenous infusion of the calcium channel blocker, diltiazem (1 mg/kg/hr), inhibited the increase in [Ca2+]i in the ischemic state and reduced the development of AGML after reinfusion. These results suggest that the increase in [Ca2+]i in the ischemic state plays an important role in the development of AGML.
Hayashi
N
N
Department of Medicine, Osaka University Medical School, Japan.
Tsujii
M
M
Itoh
T
T
Sakura
H
H
Tsuji
S
S
Tanimura
H
H
Ogihara
T
T
Yoshihara
H
H
Kawano
S
S
Sato
N
N
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004110:Diltiazem / Q000494:pharmacology; D005753:Gastric Mucosa / Q000098:blood supply* / Q000378:metabolism / Q000648:ultrastructure; D007425:Intracellular Membranes / Q000378:metabolism*; D007477:Ions; D008297:Male; D009994:Osmolar Concentration; D010762:Phosphorylase a / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D015427:Reperfusion Injury / Q000378:metabolism*
D007477:Ions; D010762:Phosphorylase a; D004110:Diltiazem; D002118:Calcium