title: A search for restriction fragment length polymorphism on the human Y chromosome.
abstract: A systematic search for restriction fragment length polymorphisms (RFLPs) on the human Y chromosome was performed. DNA samples from 16-34 individuals were screened with five restriction enzymes and 12 Y-chromosomal probes, 3 of which detect lowly repetitive sequences and 9 of which are apparently single copy in genomic DNA. None of the single-copy probes revealed any variation. The repetitive sequence probe p21A1 (DYZ?) revealed a TaqI RFLP with q = 0.05. The frequency of fixed point mutations in Y-chromosomal DNA outside the pseudoautosomal region is probably less than 1 in 18,000 bp.
authors.affiliation: Institut für Humangenetik der Universität, Göttingen, Federal Republic of Germany.
authors.forename: S
authors.initials: S
authors.lastname: Jakubiczka
authors.forename: J
authors.initials: J
authors.lastname: Arnemann
authors.forename: H J
authors.initials: HJ
authors.lastname: Cooke
authors.forename: M
authors.initials: M
authors.lastname: Krawczak
authors.forename: J
authors.initials: J
authors.lastname: Schmidtke
mesh_terms: D015139:Blotting, Southern; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; D005260:Female; D006801:Humans; D008297:Male; D010375:Pedigree; D012150:Polymorphism, Restriction Fragment Length; D014998:Y Chromosome
chemical_list: D015342:DNA Probes; D004247:DNA; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; C027046:TCGA-specific type II deoxyribonucleases