[Comparative study of the protective effects of a cold potassium cardioplegic solution verses the Collins solution in the preservation of canine hearts for 24 hours by simple immersion method].
This study was undertaken to compare the protective effects of a cold potassium cardioplegic solution (K+:23 mEq/l, Na+:25.2 mEq/l) versus the Collins solution (K+:115 mEq/l, Na+:10 mEq/l) on the preservation of isolated canine hearts for 24 hours by simple immersion. To assess the viability of the preserved hearts, recovery of left ventricular function, myocardial water content and myocardial ultrastructures were evaluated before and after preservation. Isovolumic left ventricular function was evaluated with an intra-ventricular balloon method using a preparation of an isolated, blood-perfused heart. The cardioplegic solution group (n = 9) showed significantly better recovery results of pressure developed in the left ventricular (84-76 vs. 35-37%) and dp/dt (90-52% vs. 44-39%) as compared to the Collins solution group (n = 13). Compliance of the left ventricle was well maintained in the cardioplegic solution group. The Collins solution group showed a significant increase of myocardial water content and a remarkable decrease in compliance of the left ventricle after preservation. The quantitative evaluation results of the mitochondrial injury (mitochondria score) found through electron microscopy revealed that the mitochondria of the cardioplegic solution group were less injured as compared to those of the Collins solution group. We concluded that our cold potassium cardioplegic solution offers superior protective effects on the preservation of the heart by simple immersion as compared to the Collins solution.
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D000818:Animals; D002314:Cardioplegic Solutions / Q000494:pharmacology*; D004285:Dogs; D006321:Heart / Q000502:physiology*; D006982:Hypertonic Solutions / Q000494:pharmacology*; D009926:Organ Preservation / Q000379:methods*; D011188:Potassium / Q000494:pharmacology*
D002314:Cardioplegic Solutions; C024102:Collins' solution; D006982:Hypertonic Solutions; D011188:Potassium