title: 18-Hydroxylase activity in the Y1 adrenal cell line.
abstract: 18-Hydroxylase activity, reported here for the first time in the mouse adrenal tumor cell line (Y1), was expressed in the metabolism of 11-deoxycorticosterone (DOC) and corticosterone (B). Detected after 24 h of incubation, it was more evident after 48 h and produced mostly 18-hydroxy-20 alpha-DHB from these exogenous substrates. However, 18-hydroxylation was quantitatively less significant than the metabolism of 20 alpha-reduction and 11 beta-hydroxylation (of DOC). The latter is also the predominant metabolism of progesterone in this cell line, during the conversion of cholesterol from the serum-supplemented culture media. The cytochrome P-450 11 beta activity of the Y1 cells is similar to that of the mouse in vivo which catalyzes the production of an 11 beta 18-dihydroxylated metabolite as the principal 18-hydroxylated steroid. It is different from that of other species, such as the rat and the bovine, both in terms of the ratio of 11 beta- to 18-hydroxylated metabolites and of the structure of these metabolites.
authors.affiliation: Laboratoire de Biochimie des Interactions Cellulaires, Faculté, de Sciences, Université de Bourgogne, Dijon, France.
authors.forename: L C
authors.initials: LC
authors.lastname: Ramirez
authors.forename: M
authors.initials: M
authors.lastname: Es-souni
authors.forename: P
authors.initials: P
authors.lastname: Bournot
mesh_terms: D000302:Adrenal Cortex / Q000201:enzymology*; D000818:Animals; D003345:Corticosterone / Q000378:metabolism; D019405:Cytochrome P-450 CYP11B2; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D003900:Desoxycorticosterone / Q000378:metabolism; D051379:Mice; D013250:Steroid Hydroxylases / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D003900:Desoxycorticosterone; D003577:Cytochrome P-450 Enzyme System; D013250:Steroid Hydroxylases; D019405:Cytochrome P-450 CYP11B2; D003345:Corticosterone