Maturation of the 3' end of 5-S ribosomal RNA from Escherichia coli.
The 3' ends of 5-S rRNA isolated from Escherichia coli cells were analyzed and identified after different durations of labeling with 32Pi, with and without blocking of protein synthesis. These experiments suggest that the 5-S rRNA starts as a species containing 126 nucleotides, three at each end, and that the extra nucleotides are removed from the 5' and 3' ends in parallel at comparable but different rates. Inhibition of protein synthesis with chloramphenicol blocks, in addition to the 5'-end maturation, the trimming of the extra nucleotides from the 3' end. The trimming of extra nucleotides from both ends of the 5-S rRNA is also affected by the structure of the molecular stalk of 5-S rRNA. A number of observations suggest that the trimmings from both ends are independent processes, which are carried out probably by different enzymes.
Szeberényi
J
J
Tomcsányi
T
T
Apirion
D
D
D001345:Autoradiography; D055598:Chemical Phenomena; D002621:Chemistry; D002701:Chloramphenicol / Q000494:pharmacology; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D007700:Kinetics; D008970:Molecular Weight; D009843:Oligoribonucleotides / Q000032:analysis; D012323:RNA Processing, Post-Transcriptional; D012329:RNA, Bacterial / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000378:metabolism*
D009843:Oligoribonucleotides; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D002701:Chloramphenicol