[A colorimetric method for determination of delta-bilirubin in serum using bilirubin oxidase (Trachyderma tsunodae)].
We developed a colorimetric method to measure delta-Bilirubin in human serum using bilirubin oxidase from Trachyderma tsunodae and diazo reagent. In the first step, bilirubin fractions except for delta-Bilirubin, such as diconjugated, monoconjugated and unconjugated forms, are removed by enzymatic oxidation at pH 8.0 in the presence of sodium salicylate. In the second step, delta-Bilirubin remaining after enzymatic oxidation is determined by the conventional indirect diazo method. The results obtained by this method showed intra-day CV of 2.4%, satisfactory linearity and good correlation (r = 0.93, p less than 0.001) with those obtained by high performance liquid chromatography method. This simple and rapid method was clinical useful for determining the bilirubin fractions in sera of hepatic patients.
Umaji
K
K
Okumiya
T
T
Park
K
K
Sasaki
M
M
D001487:Basidiomycota / Q000201:enzymology; D001663:Bilirubin / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D003124:Colorimetry / Q000379:methods; D006801:Humans; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; D011237:Predictive Value of Tests
D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C042401:bilirubin oxidase; D001663:Bilirubin