Rearranged beta T cell receptor genes in a helper T cell clone specific for lysozyme: no correlation between V beta and MHC restriction.
The helper T cell clone 3H.25 is specific for hen egg white lysozyme and the class II MHC molecule I-Ab. This TH cell has three rearrangements in the beta-chain gene family-a V beta-D beta-J beta 1 and a D beta 2-J beta 2 rearrangement on one homolog and a D beta 1-J beta 2 rearrangement on the other. These observations demonstrate that this functional T lymphocyte expresses only a single V beta gene segment and, accordingly, exhibits allelic exclusion of beta-chain gene expression. The rearranged 3H.25 V beta gene segment is the same as that expressed in a T helper cell specific for cytochrome c and an I-Ek MHC molecule. Thus, there is no simple correlation between the V beta gene segment and antigen specificity or MHC restriction.
Goverman
J
J
Minard
K
K
Shastri
N
N
Hunkapiller
T
T
Hansburg
D
D
Sercarz
E
E
Hood
L
L
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002999:Clone Cells / Q000276:immunology; D003574:Cytochrome c Group / Q000235:genetics; D004247:DNA / Q000235:genetics; D014644:Genetic Variation; D007135:Immunoglobulin Variable Region / Q000235:genetics; D008285:Major Histocompatibility Complex; D051379:Mice; D008810:Mice, Inbred C57BL; D009113:Muramidase / Q000276:immunology*; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000235:genetics; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D013601:T-Lymphocytes / Q000276:immunology*
D003574:Cytochrome c Group; D007135:Immunoglobulin Variable Region; D011948:Receptors, Antigen, T-Cell; D012313:RNA; D004247:DNA; D009113:Muramidase