Immunological detection of arrestin, a phototransduction regulatory protein, in the cytosol of nucleated erythrocytes.
Cytosolic extracts of trout and turkey erythrocytes were tested for their immunoreactivity with polyclonal and monoclonal antibodies to retinal arrestin (S-antigen), a cytosolic protein of photoreceptor cells involved in the desensitization of rhodopsin. After adsorption or immunoaffinity chromatography of the extracts, these antibodies specifically recognized a protein having a molecular weight similar to that of retinal arrestin. Because the G-protein-mediated transduction systems, such as visual and beta-adrenergic systems, display a high degree of structural and functional homology, the presence of arrestin-like proteins in non-photosensitive cells suggests that these proteins are involved in the transduction of chemical signals, with a possible role in receptor desensitization.
Mirshahi
M
M
INSERM U86, Institut Biomédical des Cordeliers, Paris, France.
Borgese
F
F
Razaghi
A
A
Scheuring
U
U
Garcia-Romeu
F
F
Faure
J P
JP
Motais
R
R
D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis* / Q000302:isolation & purification; D019393:Arrestin; D002467:Cell Nucleus / Q000032:analysis; D002846:Chromatography, Affinity; D003600:Cytosol / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes / Q000032:analysis*; D005136:Eye Proteins / Q000032:analysis* / Q000302:isolation & purification; D007106:Immune Sera; D007118:Immunoassay; D008565:Membrane Proteins / Q000032:analysis*; D008970:Molecular Weight; D015398:Signal Transduction; D014337:Trout; D014422:Turkeys
D000911:Antibodies, Monoclonal; D000941:Antigens; D019393:Arrestin; D005136:Eye Proteins; D007106:Immune Sera; D008565:Membrane Proteins