Establishment of a human glioma cell line bearing a homogeneously staining chromosomal region and releasing alpha- and beta-type transforming growth factors.
A human glioma cell line (YKG1), which was positively identified for glial fibrillary acidic (GFA) and S-100 proteins, was established from a surgical specimen of a patient with glioblastoma. Chromosome analysis of the cells revealed a homogeneously staining region (HSR) on a marker chromosome. The assay for transforming growth factors (TGFs) in the conditioned medium of the cell line revealed that it contained high levels of alpha- and beta-type TGFs, which might regulate the growth of glioblastoma and influence on the peritumoral tissues.
Kanno
H
H
Department of Neurosurgery, School of Medicine, Yokohama City University, Japan.
Kuwabara
T
T
Shinonaga
M
M
Chang
C C
CC
Tanaka
Y
Y
Sugio
Y
Y
Morita
H
H
Yasumitsu
H
H
Umeda
M
M
Nagashima
Y
Y
D002460:Cell Line; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005910:Glioma / Q000235:genetics*; D006801:Humans; D008875:Middle Aged; D015291:Transforming Growth Factors / Q000235:genetics*; D014407:Tumor Cells, Cultured / Q000166:cytology*
D004273:DNA, Neoplasm; D015291:Transforming Growth Factors