Isolation of monosialyated oligosaccharides from human milk and structural analysis of three new compounds.
The monosialyated oligosaccharide fraction from combined samples of human milk was fractionated by gel filtration, ion-exchange chromatography, and h.p.l.c. with triethylamine as an ion-pairing reagent. Among the twelve oligosaccharides isolated, three were new compounds for which the following structures were established on the basis of chemical analyses, f.a.b.-m.s., and n.m.r. spectroscopy.
Grönberg
G
G
Department of Carbohydrate Chemistry, University of Lund, Sweden.
Lipniunas
P
P
Lundgren
T
T
Erlansson
K
K
Lindh
F
F
Nilsson
B
B
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005643:Fucose / Q000032:analysis; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008895:Milk, Human / Q000032:analysis*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009438:Neuraminic Acids / Q000032:analysis; D009844:Oligosaccharides / Q000302:isolation & purification*
D009438:Neuraminic Acids; D009844:Oligosaccharides; C016735:sialooligosaccharides; D005643:Fucose; D005947:Glucose; D005690:Galactose