Raison d'être and structural model for the B-Z transition of poly d(G-C).poly d(G-C).
In DNA oligonucleotides crystallized in the A form, the nucleotides adopt standard conformation except for steps 5'-CpG-3' where reduced base-pair twist and a sliding motion of the base pairs along their long axes causes pronounced interstrand guanine-guanine overlap. As a consequence, torsion angles alpha, beta and gamma are consistently trans, trans, trans instead of the common-gauche, trans, +gauche. This conformation significantly increases the intraresidue distance between the guanine base and the 5'-phosphate group. A molecular model of poly d(G-C).poly d(G-C) built with these structural characteristics in the A form, which we call A2-DNA, shows that rotation of the guanosine sugar into the syn orientation is easily achieved and pushes the base pair across the helix axis. If successive guanosines are changed this way, a smooth transformation occurs to the left-handed Z-DNA. We suggest that A- and A2-DNA forms of poly d(G-C).poly d(G-C) are metastable and that the actual transition is B in equilibrium (A in equilibrium A2) in equilibrium Z-DNA.
Saenger
W
W
Institüt für Kristallographie, Freie Universität Berlin, Germany.
Heinemann
U
U
D003461:Crystallography; D004247:DNA; D008958:Models, Molecular; D009038:Motion; D009690:Nucleic Acid Conformation; D011089:Polydeoxyribonucleotides
D011089:Polydeoxyribonucleotides; C019530:poly(dC-dG); D004247:DNA