Choice of an optimal diluent for intravesical bacillus Calmette-Guerin administration.
The physical conditions, including diluent pH, salt concentration and duration of bacillus Calmette-Guerin attachment, were determined in in vitro binding assays for soluble and matrix fibronectin. Since soluble fibronectin may block attachment of bacillus Calmette-Guerin to matrix fibronectin in the bladder, the optimal conditions were determined under which matrix fibronectin-bacillus Calmette-Guerin binding was maximal and soluble fibronectin-bacillus Calmette-Guerin binding was minimal. These conditions, which were confirmed in vivo in the murine bladder model, included use of normal saline, pH 7 as diluent for bacillus Calmette-Guerin organisms, with retention of the bacillus Calmette-Guerin suspension for 2 hours.
Hudson
M A
MA
Division of Urologic Surgery, Washington University School of Medicine, St. Louis, Missouri.
Catalona
W J
WJ
Ritchey
J K
JK
Aslanzadeh
J
J
Brown
E J
EJ
Ratliff
T L
TL
D000283:Administration, Intravesical; D000818:Animals; D001500:BCG Vaccine / Q000008:administration & dosage* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005353:Fibronectins / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D051379:Mice; D010592:Pharmaceutic Aids / Q000008:administration & dosage* / Q000378:metabolism; D012995:Solubility; D013997:Time Factors; D014316:Tritium
D001500:BCG Vaccine; D005353:Fibronectins; D007457:Iodine Radioisotopes; D010592:Pharmaceutic Aids; D014316:Tritium