Kinase inhibition lengthens the period of the circadian pacemaker in the eye of Bulla gouldiana.
The inhibition of protein kinase activity by the isoquinoline sulfonamide, H-8, lengthens the period of the Bulla ocular circadian rhythm in a dose-dependent manner. In addition, the phosphorylation of 5 proteins is markedly affected by H-8. The observed correlation between H-8's period lengthening effects, and its effects on the phosphorylation of specific proteins, suggests that: (1) these proteins are candidate components regulating the period of the circadian rhythm; and (2) the daily changes in membrane potential underlying the circadian rhythm are mediated by similar mechanisms that serve to change neural function in other systems; modulation of protein kinases.
Roberts
M H
MH
Department of Biology, Clarkson University, Potsdam, NY 13676.
Bedian
V
V
Chen
Y L
YL
D000818:Animals; D002940:Circadian Rhythm; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005123:Eye / Q000187:drug effects / Q000378:metabolism; D007546:Isoquinolines / Q000494:pharmacology*; D009799:Ocular Physiological Phenomena; D009890:Opisthorchidae / Q000502:physiology*; D010750:Phosphoproteins / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism / Q000502:physiology*
D004791:Enzyme Inhibitors; D007546:Isoquinolines; D010750:Phosphoproteins; C053177:N-(2-(methylamino)ethyl)-5-isoquinolinesulfonamide; D011494:Protein Kinases