Purification and characterization of rabbit tissue factor.
Anti-human tissue factor IgG column was used to partially purify rabbit tissue factor. The rabbit tissue factor was then further purified on SDS-polyacrylamide gel electrophoresis to prepare an essentially homogeneous rabbit tissue factor. This was used as an antigen to raise a high titer, monospecific, polyclonal antiserum for rabbit tissue factor. Immunostaining of a crude Triton extract of rabbit brain acetone powder and purified tissue factor with the anti-rabbit tissue factor IgG yielded a single major band with an apparent molecular weight of 45 kD, which corresponds to the mobility on SDS-PAGE of purified rabbit tissue factor apoprotein. The anti-rabbit tissue factor IgG was coupled to Affi-Gel-15 and used as an immunoadsorbent column to purify large amounts of rabbit brain tissue factor in a rapid, one-step technique.
Rao
L V
LV
Department of Medicine, University of California, San Diego, La Jolla 92093.
Hoang
A D
AD
D000818:Animals; D001059:Apoproteins / Q000032:analysis; D001923:Brain Chemistry; D002846:Chromatography, Affinity; D007106:Immune Sera / Q000276:immunology; D011817:Rabbits; D013925:Thromboplastin / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*
D001059:Apoproteins; D007106:Immune Sera; D013925:Thromboplastin