Induction of lipid peroxidation in mice by hexavalent chromium and its relation to the toxicity.
Comparative effects of hexavalent (K2Cr2O7:Cr(VI)) and trivalent chromium (Cr(NO3)3:Cr(III)) on the development of lipid peroxidation, and the relationship between the lipid peroxidation and damage to tissues were studied using male ddY strain mice. The animals were administered with either of two chemicals at a dose of 20 mg Cr/kg by a single intraperitoneal injection. The results obtained were as follows: (1) Lipid peroxidation in the liver, as measured by the synthesis of thiobarbituric acid reactive substances (TBARS), showed a significant increase at 24 and 48 hr after Cr(VI) injection, while in the kidney it was observed only at 48 hr. In the mice administered with Cr(III), TBARS formation in the liver went down below the control levels, while no change was observed in the kidney. (2) Chromium contents in the liver and kidney showed a maximum level at 6 hr after injection of Cr(VI) and then those declined to the half of the maximum level at 48 hr, respectively. Chromium contents in the liver and kidney of the mice injected with Cr(III) were lower than those injected with Cr(VI) during the experimental period. (3) Increases of TBARS formation in the liver, chromium content in the liver and kidney, and ornithine carbamyl transferase (OCT) activity indicative of the liver cell damage, and urea nitrogen content in the serum, indicative of the kidney damage, observed at 24 hr after injection of Cr(VI) were inhibited by simultaneous injection of 100 mg/kg of L-ascorbic acid, as antichrome agent, respectively. These observations might suggest a possible causative role of lipid peroxidation in Cr(VI) toxicity.(ABSTRACT TRUNCATED AT 250 WORDS)
Susa
N
N
Ueno
S
S
Furukawa
Y
Y
Michiba
N
N
Minoura
S
S
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D002840:Chromates / Q000378:metabolism*; D002857:Chromium / Q000378:metabolism* / Q000633:toxicity; D017608:Chromium Compounds; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D015227:Lipid Peroxidation; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice / Q000378:metabolism*; D009566:Nitrates / Q000378:metabolism* / Q000633:toxicity; D009584:Nitrogen / Q000097:blood / Q000494:pharmacology; D010655:Phenylenediamines / Q000494:pharmacology; D011192:Potassium Dichromate / Q000378:metabolism* / Q000633:toxicity; D013858:Thiobarbiturates / Q000378:metabolism
D002840:Chromates; D017608:Chromium Compounds; D009566:Nitrates; D010655:Phenylenediamines; D013858:Thiobarbiturates; D002857:Chromium; C041167:chromium nitrate; C004845:N,N'-diphenyl-4-phenylenediamine; C029684:thiobarbituric acid; D009584:Nitrogen; D001205:Ascorbic Acid; D011192:Potassium Dichromate