Gal-Gal pyelonephritis Escherichia coli pili linear immunogenic and antigenic epitopes.
The linear immunogenic and antigenic structure of E. coli Gal-Gal pili from the recombinant strain HU 849 was investigated with nine synthetic peptides corresponding to regions of the pilus sequence predicted to contain hydrophilic beta-turns. Five peptides, as bovine serum albumin conjugates, were found by anti-HU 849 pilus serum and were thus designated "immunogenic epitopes." Peptides corresponding to R 25-38, R 38-50, and R 48-61 (which jointly comprise the single intramolecular disulfide loop), and R 103-116, were bound in low titer. A prominent immunogenic epitope was specified by a peptide corresponding to R 65-75. Four peptides, as thyroglobulin conjugates, elicited antisera in rabbits that bound intact HU 849 pili. These were designated "antigenic epitopes." Two prominent antigenic epitopes were localized to peptides corresponding to R 5-12 and R 93-104, whereas peptides corresponding to R 65-75 and R 119-131 represented two minor antigenic epitopes. None of the peptide antisera bound Gal-Gal pili from heterologous strains except anti-R 93-104 and anti-R 5-12. In 8 of the 10 Gal-Gal-binding pyelonephritis isolates tested, anti-R 5-12 detected a protein with an apparent molecular weight of 18,000 co-migrating with several Gal-Gal pili. Anti-R 93-104 detected a corresponding protein in 4 of 8 fecal and 7 of 12 pyelonephritis Gal-Gal-binding isolates; however, it also bound apparently unrelated proteins of higher molecular weight.
Schmidt
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O'Hanley
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Schoolnik
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D000595:Amino Acid Sequence; D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis*; D000939:Epitopes / Q000032:analysis*; D004926:Escherichia coli / Q000276:immunology* / Q000378:metabolism; D005260:Female; D010861:Fimbriae, Bacterial / Q000276:immunology* / Q000378:metabolism; D005688:Galactosamine / Q000276:immunology* / Q000378:metabolism; D005690:Galactose / Q000276:immunology* / Q000378:metabolism; D006386:Hemagglutination Tests; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D011704:Pyelonephritis / Q000276:immunology; D011817:Rabbits; D011971:Receptors, Immunologic / Q000032:analysis
D000942:Antigens, Bacterial; D000939:Epitopes; D007106:Immune Sera; D010455:Peptides; D011971:Receptors, Immunologic; C037045:galactose-N-acetylgalactosamine receptor; D005688:Galactosamine; D005690:Galactose