Molecularly cloned and expressed murine T-cell gene product is biologically similar to interleukin-3.
Clonal lines of mouse inducer ly1+ly2- inducer T-lymphocytes that depend for growth upon interleukin-2 have been demonstrated to produce a factor that stimulates colony formation by bone marrow granulocyte-macrophage (GM-CFUc) progenitor cells and replication of factor-dependent mast cell/basophil and multipotential hematopoietic cell lines in vitro. The molecularly cloned and expressed gene product for this growth factor demonstrates the following activities in vitro: using fresh bone marrow or purified subpopulations of nonadherent cells from murine continuous bone marrow cultures as target cells: stimulation of colony formation by GM-CFUc, mast cell progenitor cells, multipotential granulocyte/erythroid/megakaryocyte/macrophage progenitor cells (CFU-GEMM) colonies, erythroid progenitor cells forming macroscopic bursts (BFUe), and megakaryocyte progenitor cells (CFU-mega). The gene product also supports growth of previously reported mast cell growth-factor-dependent cell lines and several classes of interleukin-3 (IL-3)-dependent hematopoietic progenitor cell lines that are multipotential (neutrophil/basophil/eosinophil or neutrophil/basophil/erythroid); or committed to granulocyte-macrophage, or mast cell/basophil differentiation. The gene product does not detectably support replication of IL-2-dependent murine T-cell lines. The biologic activity of the gene product was inhibited greater than or equal to 90% by rabbit antisera prepared against purified interleukin-3. The data indicate that this T-cell derived lymphokine gene product is biologically very similar to interleukin-3.
Greenberger
J S
JS
Humphries
R K
RK
Messner
H
H
Reid
D M
DM
Sakakeeny
M A
MA
D000818:Animals; D001854:Bone Marrow Cells; D002999:Clone Cells; D006098:Granulocytes / Q000166:cytology; D007106:Immune Sera; D007377:Interleukin-3; D008222:Lymphokines / Q000235:genetics / Q000502:physiology*; D008264:Macrophages / Q000166:cytology; D051379:Mice; D008815:Mice, Inbred Strains; D009195:Peroxidase; D014176:Protein Biosynthesis; D013194:Staining and Labeling; D013601:T-Lymphocytes / Q000502:physiology*
D007106:Immune Sera; D007377:Interleukin-3; D008222:Lymphokines; D009195:Peroxidase