Hypervariable polymorphism of autosomal origin detected by the Y-chromosome derived probe, pHY10.
A recombinant DNA probe (pHY10) hybridizing specifically to human DNA family DYZ1, 3,000 copies of which are present on the long arm of the Y chromosome, was used for probing human genome DNA digested with various restriction enzymes. To our surprise, the probe detected a hypervariable polymorphism of autosomal origin in human DNA when digested with TaqI. None of other 12 restriction enzymes revealed polymorphic patterns. Codominant segregation of the polymorphism was established in family studies. This probe has been widely used in the detection of the Y chromosome. Its ease of availability as well as highly discriminating polymorphic pattern makes it potentially very useful for forensic and human genetic purposes.
Yokoi
T
T
Sagisaka
K
K
Nakahori
Y
Y
Nakagome
Y
Y
D015342:DNA Probes; D003720:Densitometry; D015252:Deoxyribonucleases, Type II Site-Specific; D005260:Female; D014644:Genetic Variation; D006801:Humans; D008297:Male; D012150:Polymorphism, Restriction Fragment Length; D014998:Y Chromosome
D015342:DNA Probes; D015252:Deoxyribonucleases, Type II Site-Specific; C027046:TCGA-specific type II deoxyribonucleases