Cytogenetic evidence for a clonal disorder involving CFU-GEMM, BFU-E and CFU-C in patients with myeloproliferative disorders.
We grew multilineage hemopoietic colonies in vitro from 4 patients with myeloproliferative disorders (MPD) having chromosomal aberration, and performed cytogenetic analysis of single CFU-GEMM, BFU-E and CFU-C-derived colonies on Day 14 of culture. In Patient 1 with acute myelofibrosis, CFU-GEMM and BFU-E colonies had only a 47, XX, +C karyotype. In Patient 2 with polycythemia vera, CFU-GEMM, BFU-E and CFU-C colonies all showed the same abnormal karyotype of 46, XY, -12, +der(12)t(1;12). Patients 3 and 4 had primary myelofibrosis, and their respective karyotypes were 46, XY, 13q- and 46, XX, -6, +der(6)t(1;6) in CFU-GEMM, BFU-E and CFU-C colonies. In these 4 patients, the karyotype of bone marrow or circulating mononuclear cells was identical to that of hemopoietic colonies. These results indicate a clonal origin of MPD at the level of CFU-GEMM.
Sugiyama
H
H
Ichiba
S
S
Okuno
Y
Y
Takahashi
T
T
Imura
H
H
Nakamura
K
K
Iho
S
S
Hoshino
T
T
Okada
T
T
Furukawa
H
H
D000328:Adult; D000368:Aged; D002869:Chromosome Aberrations; D003114:Colony-Forming Units Assay; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007621:Karyotyping; D008297:Male; D008875:Middle Aged; D009196:Myeloproliferative Disorders / Q000097:blood / Q000235:genetics*