title: Calcium-binding protein from mouse Ehrlich ascites-tumour cells is homologous to human calcyclin.
abstract: A Ca2+-binding protein was purified from mouse Ehrlich ascites-tumour cells. The protein forms monomers and disulphide-linked dimers, which can be separated by reverse-phase h.p.l.c. A partial amino acid sequence analysis demonstrated that the protein has an EF-hand structure. A striking homology was found to rat and human calcyclin (a member of the S-100 protein family), which is possibly involved in cell-cycle regulation.
authors.affiliation: Nencki Institute of Experimental Biology, Department of Muscle Biochemistry, Warszawa, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Kuźnicki
authors.forename: A
authors.initials: A
authors.lastname: Filipek
authors.forename: P E
authors.initials: PE
authors.lastname: Hunziker
authors.forename: S
authors.initials: S
authors.lastname: Huber
authors.forename: C W
authors.initials: CW
authors.lastname: Heizmann
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001345:Autoradiography; D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000032:analysis*; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis*; D018797:Cell Cycle Proteins; D002851:Chromatography, High Pressure Liquid; D051379:Mice; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins; D014357:Trypsin
chemical_list: D000596:Amino Acids; D002135:Calcium-Binding Proteins; D018797:Cell Cycle Proteins; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins; C489134:S100a6 protein, mouse; C489132:S100a6 protein, rat; D014357:Trypsin