title: Taipoxin-binding protein on synaptic membranes: identification by affinity labeling.
abstract: Affinity labeling techniques were used to identify the neuronal membrane molecules involved in the binding of taipoxin, a neurotoxic protein with phospholipase A2 activity. After [125I]taipoxin had bound to synaptosomes from guinea pig brain, treatment with disuccinimidyl suberate resulted in the formation of a predominant radioactive conjugate of 60,000 Da. Notexin and some other PLA2s are weakly inhibitory to this conjugation, while beta-bungarotoxin and some others are not inhibitory. The 60K conjugate was not detected when plasma membranes from several nonneuronal tissues were used. We concluded that a 45,000 Da protein specifically present in neuronal membranes is (a subunit of) the major molecule responsible for taipoxin binding.
authors.affiliation: Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan, R.O.C.
authors.forename: M C
authors.initials: MC
authors.lastname: Tzeng
authors.forename: M J
authors.initials: MJ
authors.lastname: Hseu
authors.forename: C H
authors.initials: CH
authors.lastname: Yen
mesh_terms: D000345:Affinity Labels / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D004546:Elapid Venoms / Q000378:metabolism*; D006168:Guinea Pigs; D008970:Molecular Weight; D009466:Neuromuscular Blocking Agents / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D013388:Succinimides / Q000378:metabolism*; D013570:Synaptic Membranes / Q000378:metabolism*; D013574:Synaptosomes / Q000378:metabolism
chemical_list: D000345:Affinity Labels; D002352:Carrier Proteins; D004546:Elapid Venoms; D009466:Neuromuscular Blocking Agents; D013388:Succinimides; C015523:taipoxin; D010741:Phospholipases A; D054467:Phospholipases A2; C019358:disuccinimidyl suberate