Sulfation in male reproductive organs. Bull and boar testis phenol sulfotransferases.
Phenol sulfotransferases (PST) from bull and boar testis were partially purified and characterized. A single form of PST adsorbed on DEAE-cellulose was found in the bull testis, whereas from boar testis two different peaks of PST activity were separated. The bull testis PST and both boar testis enzymes were active with p-nitrophenol and adrenalin. They all showed higher affinity to pNP than to adrenalin and were inhibited by these substrates at higher concentrations. Their optimal pH was at 8.5. Bull testis PST and boar PST II which were adsorbed on DEAE-cellulose were thermostable, whereas boar PST I was thermolabile. Those three PST forms differed in sensitivity to 2,6-dichloro-4-nitrophenol (DCNP), N-ethyl maleimide (NEM), iodoacetamide (IAA) and phenylglyoxal (PG). Bull and boar PST II were more rapidly inactivated in the presence of DCNP than boar PST I. In the presence of NEM, the--SH groups reagent, the bull phenol sulfotransferase and boar PST I lost their activity, whereas the activity of boar PST increased. Also iodoacetamide, another--SH group modificator, raised boar PST II activity and decreased boar PST I activity. DTT, which protects thiol groups, had an opposite effect on the enzymes studied than NEM. Phenylglyoxal, a reagent specific for arginine residues inhibited bull testis PST and both boar phenol sulfotransferases. Substrate protection experiments were also performed to determine the localization of reactive groups in bull and boar testis phenol sulfotransferases.
Barańczyk-Kuźma
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Department of Biochemistry, Warsaw Medical School, Poland.
Ciszewska-Piłczyńska
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D000818:Animals; D015239:Arylsulfotransferase / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D002417:Cattle; D004229:Dithiothreitol / Q000494:pharmacology; D004837:Epinephrine / Q000378:metabolism; D005033:Ethylmaleimide / Q000494:pharmacology; D005837:Genitalia, Male / Q000201:enzymology*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007460:Iodoacetamide / Q000494:pharmacology; D008297:Male; D009596:Nitrophenols / Q000378:metabolism; D010658:Phenylglyoxal; D013379:Substrate Specificity; D013552:Swine; D013737:Testis / Q000201:enzymology*
D009596:Nitrophenols; D015239:Arylsulfotransferase; D010658:Phenylglyoxal; D005033:Ethylmaleimide; D004229:Dithiothreitol; C024836:4-nitrophenol; D004837:Epinephrine; D007460:Iodoacetamide