title: A reinvestigation of the amino acid sequences of bovine, rabbit, monkey, and human myelin basic proteins.
abstract: In order to determine whether bovine, rabbit, and monkey myelin basic proteins (BPs) have the sequence Gly-His or His-Gly at positions corresponding to bovine sequence 76-77, we isolated the tryptic peptides encompassing the sequence in question in these proteins and cleaved them into dipeptides with dipeptidyl aminopeptidase I (EC 3.4.14.1). Analysis by gas chromatography/mass spectrometry of the dipeptides released showed that in no case did His follow Gly or Gly precede His. The identification of peptides Ala-Gln and His-Gly (bovine BP) and Ser-His and Gly-Arg (rabbit and monkey BPs) established the His-Gly sequence. A similar sequence analysis of tryptic peptide (80-91) of human BP confirmed the sequence Thr-Gln-Asp-Glu-Asn-Pro (80-85).
authors.forename: G E
authors.initials: GE
authors.lastname: Deibler
authors.forename: H C
authors.initials: HC
authors.lastname: Krutzsch
authors.forename: R E
authors.initials: RE
authors.lastname: Martenson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002645:Chickens; D006168:Guinea Pigs; D006801:Humans; D051379:Mice; D004676:Myelin Basic Protein / Q000235:genetics*; D002679:Pan troglodytes; D010446:Peptide Fragments / Q000032:analysis; D011817:Rabbits; D051381:Rats; D013045:Species Specificity; D013552:Swine; D014357:Trypsin
chemical_list: D004676:Myelin Basic Protein; D010446:Peptide Fragments; D014357:Trypsin