Reciprocal correlation between expression of P-glycoprotein and accumulation of rhodamine 123 in human tumors.
The aim of this investigation was to determine whether a correlation exists between expression of the multi-drug-resistance associated P-glycoprotein 170 (P-gp170) and the accumulation of rhodamine 123 in human tumors. We investigated a panel of inherently resistant cell lines originating from human kidney tumor specimens and chemotherapeutically pretreated leukemias. The expression of P-gp170 was evaluated by means of two different immunohistological methods using two monoclonal antibodies against P-gp170. We found a significant reciprocal correlation between expression of P-gp170 and accumulation of rhodamine 123. This supports the view that P-gp170 might function as efflux pump in human tumors, as previously described for experimental models.
Efferth
T
T
German Cancer Research Center, Institute of Experimental Pathology, Heidelberg.
Löhrke
H
H
Volm
M
M
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D002292:Carcinoma, Renal Cell / Q000473:pathology*; D002454:Cell Differentiation; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D006801:Humans; D007680:Kidney Neoplasms / Q000473:pathology*; D007938:Leukemia; D008562:Membrane Glycoproteins / Q000032:analysis*; D009363:Neoplasm Proteins / Q000032:analysis*; D020112:Rhodamine 123; D012235:Rhodamines; D014407:Tumor Cells, Cultured / Q000166:cytology*; D014966:Xanthenes
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D005456:Fluorescent Dyes; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins; D012235:Rhodamines; D014966:Xanthenes; D020112:Rhodamine 123