title: Gelatin particle agglutination assay for HIV antibodies: a rapid, economical modification with increased sensitivity.
abstract: Modification of a commercial gelatin particle agglutination assay for anti-HIV reduces the test time to 30 min, increases the sensitivity sevenfold without any prozoning, and maintains specificity while cutting the cost of the test by 90%. The modification involves a tenfold dilution of the gelatin particles, which are added to a dilution of test serum in a 'V' well standard microplate. After incubation, plates are centrifuged briefly and allowed to stand at an inclination of 70 degrees until positive and negative reactions are clearly distinguishable within approximately 15 min.
authors.forename: J A
authors.initials: JA
authors.lastname: Barbara
authors.forename: R
authors.initials: R
authors.lastname: Salker
authors.forename: P
authors.initials: P
authors.lastname: Challis
authors.forename: M
authors.initials: M
authors.lastname: Contreras
mesh_terms: D000372:Agglutination Tests; D005780:Gelatin; D015483:HIV Antibodies / Q000032:analysis*; D006801:Humans
chemical_list: D015483:HIV Antibodies; D005780:Gelatin