Rates of mutation to growth factor autonomy and tumorigenicity differ in hematopoietic stem and precursor cells expressing the multilineage colony-stimulating factor gene.
At least two separate but interdependent events are required to attain autonomous growth as a consequence of ectopic expression of the multilineage colony-stimulating factor gene in hematopoietic progenitor cells. The rate at which the second event occurs is more than 3 orders of magnitude higher in precursor cell lines (FDC-P1 or FDC-P2) than in stem cell lines (FDC-Pmix). Autonomous, but not density-dependent, growth is tightly coupled to tumorigenicity in precursor cells; however, neither growth-factor-independent nor autonomously growing stem cell lines are tumorigenic.
Laker
C
C
Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Universität Hamburg, Federal Republic of Germany.
Kluge
N
N
Stocking
C
C
Just
U
U
Franz
M J
MJ
Ostertag
W
W
DeLamarter
J F
JF
Dexter
M
M
Spooncer
E
E
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D003115:Colony-Stimulating Factors / Q000235:genetics* / Q000276:immunology / Q000494:pharmacology; D015870:Gene Expression; D005822:Genetic Vectors; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D007106:Immune Sera; D007700:Kinetics; D051379:Mice; D009154:Mutation
D003115:Colony-Stimulating Factors; D007106:Immune Sera