The effect of asparagine and adenine on the glutamine requirement for growth of human peripheral lymphocytes.
Quantitative growth responses of lymphocytes directly isolated from individual subjects in a newly developed chemically-defined, protein-free medium are used to demonstrate that supplements of both L-asparagine and a purine source, but neither alone, significantly reduce the quantitative requirement for L-glutamine for growth. This system is useful for exploring individual differences in quantitative glutamine requirements and adequacy of asparagine and purine biosynthesis.
Pettit
F H
FH
Clayton Foundation Biochemical Institute, Department of Chemistry, University of Texas, Austin 78712.
Boghossian
J O
JO
Shive
W
W
D000225:Adenine / Q000378:metabolism*; D001216:Asparagine / Q000378:metabolism*; D002455:Cell Division; D002478:Cells, Cultured; D005973:Glutamine / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008214:Lymphocytes / Q000166:cytology* / Q000378:metabolism
D005973:Glutamine; D001216:Asparagine; D000225:Adenine