[The effects of nicardipine on morphological changes and calcium accumulation during ischemic hypoxia in pyramidal nerve cells in the rat cerebral cortex].
The morphological changes and the calcium accumulation were studied in the rat pyramidal nerve cells which had undergone 5% oxygen inhalation and bilateral carotid artery clamping for 15 minutes, using combined methods of electron and the X-ray microanalytical microscopes. To study the protective effects of nicardipine on brain hypoxia, the same techniques were also used after the inhalation. In the 5% oxygen inhalation group, the chromatin clamping in the nucleus was observed, but calcium ions could not be detected in the neuron using the X-ray microanalytical techniques. In the carotid clamping groups under 5% oxygen inhalation, severe nuclear change and mitochondrial swelling were observed and calcium ions were detected in the mitochondrion. After administrations of nicardipine under 5% oxygen inhalation and under carotid clamping with 5% oxygen, similar morphological changes were recognized by the electron microscope as compared to the non-administration groups. The rate of calcium ion detection was not different significantly (P less than 0.05) between the nicardipine administration and the non-administration groups. It is, therefore, concluded that notable protective effects of nicardipine on ischemic hypoxia were not recognized in this experiment.
Yamazaki
M
M
Yamamoto
M
M
Masuda
A
A
Ito
Y
Y
Kawahara
M
M
D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism / Q000473:pathology; D002118:Calcium / Q000378:metabolism*; D002534:Hypoxia, Brain / Q000378:metabolism / Q000473:pathology; D008297:Male; D009529:Nicardipine / Q000494:pharmacology*; D011712:Pyramidal Tracts / Q000378:metabolism / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains
D009529:Nicardipine; D002118:Calcium