Human and canine gene homologs of porcine brain natriuretic peptide.
A cDNA clone encoding porcine brain natriuretic peptide (BNP) precursor was used to probe a canine genomic DNA library for homologous sequences. A unique clone was obtained (D1) which encoded a peptide homologous to porcine BNP. A 4.0 kB Hind III fragment of D1 containing the entire BNP precursor coding region was hybridized against blots of EcoRI digested human genomic DNA. A 7.0 kB hybridizing band was observed, which was subsequently subcloned and sequenced. This human clone, H1, also contained a sequence homologous to porcine BNP precursor. Transcription of the human BNP gene was confirmed by detection of BNP-specific sequences amplified from human atrial RNA by polymerase chain reaction. These results suggest that both BNP and ANP are present and function in a wide variety of mammalian species.
Seilhamer
J J
JJ
California Biotechnology Inc., Mountain View 94043.
Arfsten
A
A
Miller
J A
JA
Lundquist
P
P
Scarborough
R M
RM
Lewicki
J A
JA
Porter
J G
JG
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004285:Dogs; D015723:Gene Library; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D020097:Natriuretic Peptide, Brain; D009419:Nerve Tissue Proteins / Q000235:genetics*; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D011498:Protein Precursors / Q000235:genetics; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013552:Swine
D009419:Nerve Tissue Proteins; D015345:Oligonucleotide Probes; D011498:Protein Precursors; D020097:Natriuretic Peptide, Brain; D004247:DNA