The molecular specificities of DS-specific reagents: an MB3-like monoclonal antibody isolates two DS molecules.
HLA-DS molecules are the human homologues of murine I-A molecules. The molecular specificities of four anti-DS reagents were examined by two-dimensional gel electrophoresis in light of the recent evidence that HLA-DR homozygous cell lines express at least two DS molecules. Each of the DS-specific reagents, including the IVD12 monoclonal antibody which defines an MB3-like determinant, isolated at least two DS molecules from a DR5 homozygous cell line. Because previous studies of this cell line documented that anti-MB3 allosera isolated only one DS molecule, these data indicate that there is heterogeneity of the molecular specificities of anti-Ia reagents that are specific for the MB3 allodeterminant or the MB3-like determinant in population studies.
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D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D002460:Cell Line; D000939:Epitopes / Q000302:isolation & purification; D006683:HLA-DQ Antigens; D000949:Histocompatibility Antigens Class II / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006683:HLA-DQ Antigens; D000949:Histocompatibility Antigens Class II