In vitro proliferation of murine spleen cells: I. Strain variation of response to medium from cultures of EL-4 cells.
Proliferative responses of murine lymphoid cells were elicited in vitro with supernatant fluid from cultures of EL-4 thymoma cells stimulated with phorbol ester. It was demonstrated that such responses depend on IL-2 contained in the supernatant fluid, but reflect co-stimulation with IL-2 and phorbol ester. Striking differences in the magnitude of proliferative responses of spleen, splenic T lymphocytes, thymus and bone marrow cells from various strains were observed. Three classes of responders could be identified. The differences in responsiveness, at least in part, reflected differences in the frequency of responsive cells and were genetically controlled by codominant alleles of two independent somatic genes.
Dowjat
W K
WK
Department of Microbiology, State University of New York, Buffalo 14214.
Zhou
P
P
Quackenbush
L J
LJ
Gorzynski
T
T
Zaleski
M B
MB
D000818:Animals; D002455:Cell Division; D003470:Culture Media; D005260:Female; D005838:Genotype; D007376:Interleukin-2 / Q000494:pharmacology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D013154:Spleen / Q000166:cytology*; D013601:T-Lymphocytes / Q000276:immunology; D013945:Thymoma / Q000473:pathology; D013953:Thymus Neoplasms / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000166:cytology* / Q000502:physiology
D003470:Culture Media; D007376:Interleukin-2