Determination of thiobarbituric acid-reactive substances in oxidized lipids by high-performance liquid chromatography with a postcolumn reaction system.
A new high-performance liquid chromatography procedure with a postcolumn reaction system for determination of free malondialdehyde (MDA) and other thiobarbituric acid-reactive substances (TBA-RS) in oxidized lipids in vitro has been developed. Using this procedure, both thermally oxidized methyl linoleate and the degradation products of methyl linoleate hydroperoxides revealed many kinds of lipophilic TBA-RS, but no free MDA was detected on the high-performance liquid chromatography. Similarly, oxidized methyl arachidonate also produced certain kinds of TBA-RS in the lipophilic phase and a small amount of free MDA in the hydrophilic phase. These results indicate that lipophilic TBA-RS produced in oxidized lipids in vitro are major TBA-RS and that the production of free MDA is small, even though the degree of lipid oxidation has previously been estimated as an MDA equivalent measured by the TBA colorimetric test.
Yoden
K
K
Showa College of Pharmaceutical Sciences, Tokyo, Japan.
Iio
T
T
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008041:Linoleic Acids / Q000032:analysis; D008055:Lipids / Q000032:analysis*; D008315:Malondialdehyde / Q000302:isolation & purification; D008862:Microsomes, Liver / Q000032:analysis; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains; D013053:Spectrophotometry; D013858:Thiobarbiturates / Q000378:metabolism*
D008041:Linoleic Acids; D008055:Lipids; D013858:Thiobarbiturates; C005575:methyl linoleate; D008315:Malondialdehyde; C029684:thiobarbituric acid