Nuclear matrix-associated DNA methylase.
Previous procedures for the extraction of DNA methylase (EC 2.1.1.37) from nuclei of mouse ascites cells have involved the use of buffers containing 0.2M NaCl. Whilst such 'soluble' methylase accounts for the bulk (70-80%) of DNA methylase activity a further portion of activity is detectable in a 'bound' form firmly associated with 2 M NaCl-resistant nuclear matrix-like structures. This association, which in part requires continuing DNA replication and protein synthesis, can, however, be disrupted in vitro with high concentrations of ammonium sulphate, and the enzymic properties of the 'bound' form of DNA methylase are similar to those described for the 'soluble' form.
Burdon
R H
RH
Qureshi
M
M
Adams
R L
RL
D000645:Ammonium Sulfate; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D001374:Azacitidine / Q000031:analogs & derivatives / Q000494:pharmacology; D002287:Carcinoma, Krebs 2 / Q000201:enzymology*; D002467:Cell Nucleus / Q000201:enzymology*; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000302:isolation & purification* / Q000378:metabolism; D000077209:Decitabine; D007700:Kinetics; D007739:L Cells / Q000201:enzymology; D008745:Methylation; D008780:Methyltransferases / Q000302:isolation & purification*; D051379:Mice; D012995:Solubility
D000970:Antineoplastic Agents; D000077209:Decitabine; D008780:Methyltransferases; D004248:DNA (Cytosine-5-)-Methyltransferases; D001374:Azacitidine; D000645:Ammonium Sulfate