The membrane protein compositions of lipopolysaccharide- or lipoprotein-activated B lymphoblasts from C57BL LPS-responder and non-responder mice are qualitatively indistinguishable.
Membrane proteins from murine lymphoblasts enriched by the Triton-X114 procedure were analysed by 2-dimensional gel electrophoresis to reveal proteins differentially expressed in mitogen-reactive subpopulations of B cells. The protein patterns from C57BL/6 normal and nude mice and from the B10.Sc.Cr LPS-non-responder strain, activated with lipopolysaccharide (LPS) or an analogue of lipoprotein, were virtually identical when run under strictly parallel conditions. These experiments raise the question as to whether mitogen receptors, serologically and functionally found to be membrane-bound, do exist as membrane proteins.
Kleine
B
B
Institut für Immunbiologie, Universität Freiburg, FRG.
Biesert
L
L
Bessler
W G
WG
D000818:Animals; D001402:B-Lymphocytes / Q000032:analysis*; D005260:Female; D055666:Lipopeptides; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008074:Lipoproteins / Q000494:pharmacology*; D008213:Lymphocyte Activation; D008297:Male; D008565:Membrane Proteins / Q000032:analysis*; D051379:Mice; D008810:Mice, Inbred C57BL; D009842:Oligopeptides / Q000494:pharmacology
D055666:Lipopeptides; D008070:Lipopolysaccharides; D008074:Lipoproteins; D008565:Membrane Proteins; D009842:Oligopeptides; C038293:2,3-bis(palmitoyloxy)-2-propyl-N-palmitoyl-cysteinyl-seryl-seryl-asparaginyl-alanine