[Cytoskeletal regulation of the sensitivity of brain synaptosomes to the depolarizing action of veratrine].
Using a radioactive permeant cation 3H-tetraphenylphosphonium, the sensitivity of rat brain synaptosomes to depolarizing action of veratrine, which specifically opens the sodium channels, was compared before and after destruction of microtubules and microfilaments. Depolymerization of microtubules with colchicin and vinblastine decreased an apparent affinity of veratrine to its receptor in the channel, while destruction of microfilaments with cytochalasin B had the opposite effect. Colchicine did not change allosteric interactions between the receptor for veratrine and that for scorpion venom in the sodium channel evaluated by the ability of scorpion venom to facilitate veratrine-induced depolarization of synaptosomes. It is suggested that two main cytoskeleton subsystems control the state of sodium channels in the nerve ending.
Rakovich
A A
AA
Aksentsev
S L
SL
Okun'
I M
IM
Konev
S V
SV
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000502:physiology; D003599:Cytoskeleton / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D007202:Indicators and Reagents; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009861:Onium Compounds; D009943:Organophosphorus Compounds; D051381:Rats; D013574:Synaptosomes / Q000187:drug effects* / Q000502:physiology; D014316:Tritium; D014702:Veratrine / Q000494:pharmacology*
D007202:Indicators and Reagents; D009861:Onium Compounds; D009943:Organophosphorus Compounds; D014316:Tritium; D014702:Veratrine; C013289:tetraphenylphosphonium
Reguliatsiia tsitoskeletom chuvstvitel'nosti sinaptosom mozga k depoliarizuiushchemu deĭstviiu veratrina.