[Structure of carbohydrate chains of the riboflavin-binding glycoprotein of chicken egg protein. II. 1H-NMR (500 MHz) spectroscopy of the major neutral oligosaccharides].
Reductive cleavage of riboflavin-binding glycoprotein from hen egg white (RF-GPw) with LiBH4/tert-BuOH followed by NaBH4/NaOH treatment gave rise to oligosaccharide alditols, fractionated by a successive HPLC on muBondapak C18 and Zorbax NH2 columns. Seven main individual oligosaccharide alditols were isolated and their structure was investigated by 1H NMR 500-MHz spectroscopy. The structure and relative content of the main oligosaccharide chains were proved to be identical in RF-GPw and ovomucoid. Structure of polypeptide chains and their molecular weight, number of glycosylation sites and their structure had little or no effect on the glycosylation pattern in both glycoproteins. HPLC of the oligosaccharide alditols from another egg white glycoprotein, ovotransferrin, also revealed its high microheterogeneity and close resemblance to those of ovomucoid and RF-GPw.
Piskarev
V E
VE
Sepetov
N F
NF
likhosherstov
L M
LM
Galenko
E L
EL
Derevitskaia
V A
VA
Kochetkov
N K
NK
D000818:Animals; D002240:Carbohydrate Sequence; D002352:Carrier Proteins; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D003207:Conalbumin / Q000302:isolation & purification; D004527:Egg Proteins / Q000302:isolation & purification; D004529:Egg White; D005260:Female; D006031:Glycosylation; D009682:Magnetic Resonance Spectroscopy; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D009844:Oligosaccharides; D010059:Ovomucin / Q000302:isolation & purification; D010084:Oxidation-Reduction; D012256:Riboflavin / Q000378:metabolism
D002352:Carrier Proteins; D004527:Egg Proteins; D026901:Membrane Transport Proteins; D009844:Oligosaccharides; C019079:riboflavin-binding protein; D003207:Conalbumin; D010059:Ovomucin; D012256:Riboflavin
Struktura uglevodnykh tsepeĭ riboflavinsviazyvaiushchego glikoproteina belka kurinogo iaĭtsa. II. Spektroskopiia 1H-YaMR (500 MGts) glavnykh neĭtral'nykh oligosakharidov.