Probing of DNA structure with osmium tetroxide. Effect of ligands.
Fourteen OsO4 complexes with different ligands were tested as probes of DNA structure. Of these complexes, only OsO4-2,2'-bipyridine (Os-bipy), OsO4-bathophenanthrolinedisulfonic acid (Os-bpds) and OsO4-N,N,N',N'-tetramethylenediamine (Os-TMEN) site-specifically modified the ColE1 cruciform in a supercoiled plasmid pColIR215 at millimolar concentrations. Os-bipy, Os-bpds and Os-TMEN also displayed site-specific modification of the B-Z junctions in the supercoiled plasmid pRW751 containing (dC-dG)n inserts.
Palecek
E
E
Institute of Biophysics, Czechoslovak Academy of Sciences, Brno.
Boublíková
P
P
Nejedlý
K
K
D003086:Bacteriocin Plasmids; D004269:DNA, Bacterial / Q000032:analysis / Q000378:metabolism; D004278:DNA, Superhelical; D015245:Deoxyribonuclease BamHI; D004586:Electrophoresis; D008024:Ligands; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D009992:Osmium; D009993:Osmium Tetroxide; D010957:Plasmids
D004269:DNA, Bacterial; D004278:DNA, Superhelical; D008024:Ligands; D009992:Osmium; D015245:Deoxyribonuclease BamHI; D009993:Osmium Tetroxide