title: A fast atom bombardment-mass spectrometric method to quantitate lysophosphatidylserine in rat brain.
abstract: A method to quantitate lysophosphatidylserine by fast atom bombardment-mass spectrometry using 1-hexa-decanoyl-sn-glycero-3-phospho-L-serine as internal standard is described. The standard curve is linear with a correlation coefficient r2 = 0.999 from 10 to 1000 ng. This curve has been used to quantitate LPS in rat brain using phosphorus assay as a test control. We found 475 +/- 70 ng of LPS in 1 mg of tissue (n = 3). This method presents advantages due to its sensitivity and its capability to give molecular information of the unmodified compound.
authors.affiliation: Istituto di Ricerche Farmacologiche Mario Negri, Bergamo, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Benfenati
authors.forename: G
authors.initials: G
authors.lastname: De Bellis
authors.forename: S
authors.initials: S
authors.lastname: Chen
authors.forename: L
authors.initials: L
authors.lastname: Bettazzoli
authors.forename: R
authors.initials: R
authors.lastname: Fanelli
authors.forename: M T
authors.initials: MT
authors.lastname: Tacconi
authors.forename: G
authors.initials: G
authors.lastname: Kirschner
authors.forename: G
authors.initials: G
authors.lastname: Pege
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000032:analysis*; D008246:Lysophospholipids / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods; D051381:Rats
chemical_list: D008246:Lysophospholipids; C025059:lysophosphatidylserine