The effect of GD3 ganglioside obtained from bovine lymphosarcoma on bovine normal mononuclear cell.
The effect of immunological function of GD3 on normal bovine lymphocyte was examined with various in vitro assay systems. The GD3 level in sera from enzootic bovine leukemia (EBL) cattle was significantly increased compared with that of normal cattle (EBL: 0.62 +/- 0.24 microgram/ml; normal cattle: 0.33 +/- 0.09 microgram/ml, P less than 0.05). Lymphocyte blastogenesis elicited by concanavalin A was inhibited by addition of a 50 micrograms/ml concentration, or more, of GD3. Inhibitory effect of GD3 in IL-2-dependent T cell line and EBL tumor cell line was hardly observed compared with normal peripheral blood mononuclear cells. GD3 also inhibited mixed lymphocyte reaction and allo cytotoxic T lymphocyte reaction.
Yasutomi
Y
Y
Department of Veterinary Microbiology, Rakuno Gakuen University, Ebets.
Onuma
M
M
Watarai
S
S
Yasuda
T
T
Kirisawa
R
R
Kawakami
Y
Y
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology*; D003208:Concanavalin A / Q000494:pharmacology; D003601:Cytotoxicity Tests, Immunologic; D005732:Gangliosides / Q000097:blood / Q000494:pharmacology*; D008213:Lymphocyte Activation; D008228:Lymphoma, Non-Hodgkin / Q000662:veterinary*; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014407:Tumor Cells, Cultured
D005732:Gangliosides; D003208:Concanavalin A; C026226:ganglioside, GD3