title: Molecular diagnosis of chronic myeloid leukemia using a 3'bcr probe.
abstract: Chronic myeloid leukemia (CML) is characterized by the Philadelphia chromosome which results from a reciprocal (9; 22) translocation, with the protooncogene c-abl moving from chromosome 9 to 22 and juxtaposed to the proximal bcr. Breakpoints on chromosome 22 are localized within 5.8 kb of the breakpoint cluster region (bcr). We have assessed the feasibility of using a 3'bcr probe for molecular diagnosis of CML. Thirty patients with Ph chromosome negative or positive CML were studied by Southern blot. A bcr rearrangement was seen to be present in all but one patient with Ph+CML. A case of Ph negative CML showed a bcr rearrangement. We conclude that this technique is efficient for molecular diagnosis of CML.
authors.affiliation: Département d'Hématologie et d'Immunologie, CHU, Saint-Etienne, Hôpital Nord, Saint-Priest-en-Jarez, France.
authors.forename: M F
authors.initials: MF
authors.lastname: Berthéas
authors.forename: M
authors.initials: M
authors.lastname: Vindimian
authors.forename: G
authors.initials: G
authors.lastname: Ollagnon
authors.forename: G
authors.initials: G
authors.lastname: Pomier
authors.forename: J
authors.initials: J
authors.lastname: Jaubert
authors.forename: C
authors.initials: C
authors.lastname: Vasselon
authors.forename: J
authors.initials: J
authors.lastname: Reynaud
authors.forename: J C
authors.initials: JC
authors.lastname: Heally
authors.forename: J C
authors.initials: JC
authors.lastname: Le Petit
authors.forename: C P
authors.initials: CP
authors.lastname: Brizard
mesh_terms: D015139:Blotting, Southern; D015342:DNA Probes; D015321:Gene Rearrangement / Q000235:genetics; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000175:diagnosis* / Q000235:genetics
chemical_list: D015342:DNA Probes