title: Electron-probe X-ray microanalysis of sodium ion content in vascular smooth muscle cells from spontaneously hypertensive and normotensive rats.
abstract: In aortic smooth muscle cells from 12 spontaneously hypertensive rats (SHR) of the Münster strain and 11 normotensive Wistar-Kyoto rats (WKY), the intracellular Na+ content was measured by electron-probe microanalysis. Measurements were performed in aortic cryosections 3 microns thick; the Na+ content was 12.5 +/- 2.4 g/kg dry weight in SHR versus 6.96 +/- 1.1 g/kg dry weight in WKY (P less than 0.01). Thus, aortic smooth muscle cells from SHR are characterized by a markedly elevated intracellular Na+ content compared with normotensive cells. This may either be due to genetically determined disturbances in transmembrane Na+ transport or to a circulating factor affecting Na+ transport. Cellular Na+ handling may be disturbed in SHR aortic smooth muscle as it is in hypertensive blood cells.
authors.affiliation: Medizinische Poliklinik, Universität Münster, Federal Republic of Germany.
authors.forename: W
authors.initials: W
authors.lastname: Zidek
authors.forename: E R
authors.initials: ER
authors.lastname: Krefting
authors.forename: K
authors.initials: K
authors.lastname: Kisters
authors.forename: C
authors.initials: C
authors.lastname: Spieker
authors.forename: K H
authors.initials: KH
authors.lastname: Rahn
mesh_terms: D000818:Animals; D001011:Aorta; D004577:Electron Probe Microanalysis; D006973:Hypertension / Q000378:metabolism*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D012964:Sodium / Q000032:analysis*
chemical_list: D012964:Sodium