[Development of a serum-free medium and primary culture of human renal cell carcinomas by serum-free culture].
Ovomacroglobulin and LDL fraction of egg yolk were effective to maintain the growth of established human cancer cell lines (Kato III, QG-90, Raji). A serum-free medium (BEM-841) containing 10 mM HEPES, 50 micrograms/ml of ovomacroglobulin, 0.1% (V/V) of LDL fraction of egg yolk, 10(-8) M catalase, 5 micrograms/ml of insulin, 5 micrograms/ml of transferrin, 5 ng/ml of selenium, 1 mg/ml of BSA, and 0.5% (V/V) of trace elements was developed. Primary culture of renal cell carcinoma cells was successful in 3 cases out of 6 by using BEM-841 medium without overgrowth of the fibroblast.
Ito
H
H
Yamaguchi
K
K
Kotake
T
T
Matsuzaki
O
O
D000368:Aged; D002292:Carcinoma, Renal Cell / Q000473:pathology*; D008078:Cholesterol, LDL; D003470:Culture Media; D006801:Humans; D007680:Kidney Neoplasms / Q000473:pathology*; D008259:Macroglobulins; D008297:Male; D008875:Middle Aged; D014407:Tumor Cells, Cultured; D000511:alpha-Macroglobulins
D008078:Cholesterol, LDL; D003470:Culture Media; D008259:Macroglobulins; D000511:alpha-Macroglobulins; C036828:ovomacroglobulin