[Amino acid sequence of various peptides of tryptophanyl-tRNA-synthetase from bovine pancreas].
Method of isolation of the bovine pancreas tryptophanyl-tRNA synthetase is improved and a protein with greater than or equal to 99% purity, according to PAGE-SDS, is obtained. The pure enzyme is digested with clostripain and the hydrolysate is separated by FPLC anion-exchange chromatography followed by reversed phase HPLC. Amino acid sequences of 6 individual peptides, including C-terminal one, were determined by the automated Edman degradation. A peptide is also revealed which is encoded with the low degeneracy.
Zargarova
T A
TA
Kovaleva
G K
GK
Favorova
O O
OO
Levina
N B
NB
Telezhinskaia
I N
IN
D000595:Amino Acid Sequence; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D008969:Molecular Sequence Data; D010179:Pancreas / Q000201:enzymology*; D010455:Peptides / Q000235:genetics* / Q000302:isolation & purification; D011487:Protein Conformation; D014369:Tryptophan-tRNA Ligase / Q000302:isolation & purification / Q000378:metabolism*
D010455:Peptides; D000604:Amino Acyl-tRNA Synthetases; D014369:Tryptophan-tRNA Ligase
Aminokislotnaia posledovatel'nost' nekotorykh peptidov triptofanil- tRNK-sintetazy iz podzheludochnoĭ zhelezy krupnogo rogatogo skota.