title: A method for isolating and patch-clamping single mammalian taste receptor cells.
abstract: Individual taste receptor cells were isolated from the tongue of the mouse by enzymatic treatment followed by mechanical dissociation. The cells were morphologically identical with taste cells from amphibians. Whole-cell voltage-clamp recordings indicated that the murine taste cells possess a variety of voltage-dependent inward and outward currents. Delayed rectifier currents were blocked by denatonium benzoate, one of the most bitter compounds known. This preparation should permit a detailed electrophysiologcal investigation of taste transduction in mammals at the level of taste receptor cells.
authors.affiliation: Monell Chemical Senses Center, School of Dental Medicine, Philadelphia, PA 19104.
authors.forename: A I
authors.initials: AI
authors.lastname: Spielman
authors.forename: I
authors.initials: I
authors.lastname: Mody
authors.forename: J G
authors.initials: JG
authors.lastname: Brand
authors.forename: G
authors.initials: G
authors.lastname: Whitney
authors.forename: J F
authors.initials: JF
authors.lastname: MacDonald
authors.forename: M W
authors.initials: MW
authors.lastname: Salter
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000379:methods*; D004558:Electric Stimulation; D008564:Membrane Potentials; D051379:Mice; D011984:Sensory Receptor Cells / Q000502:physiology*; D013649:Taste / Q000502:physiology*; D014059:Tongue / Q000166:cytology*