Histochemical localization of cysteine-rich proteins by tissue printing on nitrocellulose.
A rapid technique for the histochemical localization of cysteine-rich proteins in plant tissues was developed. It is based on the immediate transfer of proteins to nitrocellulose membranes when a fresh cut organ is pressed against the membrane surface. The print was labeled for cysteine-rich proteins by reduction and alkylation of cysteinyl residues with dansylated iodoacetamide [N-iodoacetyl-N'-(-5-sulfo-1-naphthyl)ethylenediamine]. The S-carboxymethylated proteins were visualized by their fluorescence when excited with 360 nm light.
Pont-Lezica
R F
RF
Department of Biology, Washington University, St. Louis, Missouri 63130.
Varner
J E
JE
D023181:Antimicrobial Cationic Peptides; D003101:Collodion; D003545:Cysteine / Q000032:analysis*; D006651:Histocytochemistry / Q000379:methods*; D001467:Hordeum / Q000032:analysis; D037102:Lectins / Q000032:analysis; D008567:Membranes, Artificial; D009282:Naphthalenesulfonates; D010084:Oxidation-Reduction; D037121:Plant Lectins; D010940:Plant Proteins / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D011198:Solanum tuberosum / Q000032:analysis
D023181:Antimicrobial Cationic Peptides; D037102:Lectins; D008567:Membranes, Artificial; D009282:Naphthalenesulfonates; D037121:Plant Lectins; D010940:Plant Proteins; D011506:Proteins; C006421:1,5-I-AEDANS; D003101:Collodion; C016085:purothionin; D003545:Cysteine