A possible role of actin in the mechanical control of the cell cycle.
Sail-sheet Cultures (SSC) are those in which the cells are i) grown within the meshes of inert grids ii) exposed to nutrients from most sides iii) attached to one another only at the edges like sail of a yacht (hence, the name 'sail-sheet') and iv) have the advantage of three-dimensional structure similar to an in vivo situation. We grew fibroblasts from chicken heart explants as SSC and studied the effect of mechanical stretching on the F-actin content of these cells. This study was designed to investigate the hypothesis that the effect of tension on the cell cycle may be channeled through the microfilaments. Data from this preliminary study suggested that short-term mechanical stretching of sail-sheets, using low frequency tension (1.0 Hz), diminishes F-actin. Thus, it may be possible to relate the decrease in the F-actin content of these cells to the slowing down of their locomotory activity, possible rounding up, and division. This study might contribute to the understanding of the mechanical control of the cell cycle and be of relevance in the phenomena such as healing of wounds and control of the cell division in tumors.
Tripathi
S C
SC
Center for Environmental Health Sciences, Massachusetts Institute of Technology, Cambridge 02139.
D000199:Actins / Q000502:physiology*; D000818:Animals; D002453:Cell Cycle; D002478:Cells, Cultured; D002645:Chickens; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D009206:Myocardium / Q000166:cytology / Q000378:metabolism
D000199:Actins