Ragged-red fibres detected in paraffin sections by a monoclonal antibody to inner mitochondrial membrane.
An immunohistochemical method is reported using the M-II68 monoclonal antibody, which detects mitochondrial accumulations ("ragged-red fibres") in routinely processed (formalin-fixed, paraffin-embedded) muscle tissue. Ten cases with electron-microscopically and histochemically proven mitochondrial myopathy featured 4% to 24% ragged-red fibres. In a series of 50 muscle biopsies without mitochondrial myopathy, scattered ragged-red fibres (less than 0.1%) were present in a few normal and pathological muscles. The immunohistochemical method is specific for mitochondria, does not require frozen tissue and permits rapid examination of large areas.
Paulus
W
W
Institut für Hirnforschung, Universität Tübingen, Federal Republic of Germany.
Peiffer
J
J
Roggendorf
W
W
Wiedmann
K H
KH
Schröder
J M
JM
D000911:Antibodies, Monoclonal; D006801:Humans; D007150:Immunohistochemistry; D008931:Mitochondria, Muscle / Q000276:immunology / Q000473:pathology*; D009135:Muscular Diseases / Q000473:pathology*
D000911:Antibodies, Monoclonal