[Comparative study on human chorionic gonadotropin receptor in normal human ovary with ovarian tumors].
Human Chorionic Gonadotropin (HCG) is major physiological luteotropic factors for the human corpus luteum. The observations strongly suggest that the human ovary possesses a gonadotropin receptor in the cell membrane. We studied the HCG receptor in normal human ovary and ovarian tumors. Twenty-three human ovarian specimens and 16 ovarian tumor specimens were obtained from women patients having gynecological surgery. Ovaries were homogenized and sonicated. The homogenates were centrifuged at 2000 g for 15 min. After sucrose density gradient ultracentrifugation (78,000 g, 4 h), two fractions were collected from layer of 33% and interface between 33% and 37%. Thirty micrograms of ovarian protein, 8 ng 125I-HCG and unlabeled HCG in a final volume of 0.5 ml of 0.05 mol/L Tris buffer were incubated at 30 degrees C for 2 h. The results were shown in the table.
Chen
M L
ML
Du
Z L
ZL
Lan
T H
TH
Cao
Z Y
ZY
Han
S W
SW
Lei
Y D
YD
Li
L
L
D001665:Binding Sites; D003536:Cystadenocarcinoma / Q000032:analysis*; D005260:Female; D006801:Humans; D010051:Ovarian Neoplasms / Q000032:analysis*; D010053:Ovary / Q000032:analysis*; D011967:Receptors, Gonadotropin / Q000032:analysis* / Q000378:metabolism
D011967:Receptors, Gonadotropin