[The role of divalent cations in DNA endonucleolysis catalyzed by restrictases].
Electrophoretic analysis of products obtained after hydrolysis of phage lambda DNA by means of restrictase Pae I was carried out after preincubation of DNA or the enzyme with Mg2+ as well as after preincubation of DNA simultaneously with the enzyme and the subsequent addition of the required components into the experimental samples. The analysis showed that Mg2+ were apparently not required for the enzyme-substrate complex formation and caused destabilization of the complex. Restrictase Pae I was in active form when Mg2+ was substituted by Mn2+, Ca2+, Zn2+, Co2+ but not by Cd2+, Cu2+ or Ni2+. Experiments with o-phenanthroline showed that Zn2+ cations are of importance in catalytic activity of restrictase Pae I. Possible functions of Zn2+ in protein-nucleic acids recognition are discussed.
Sokolov
N N
NN
Fitsner
A B
AB
Anikeĭcheva
N V
NV
Kovalenko
N A
NA
D002413:Cations, Divalent / Q000494:pharmacology*; D004247:DNA / Q000187:drug effects / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006868:Hydrolysis; D066298:In Vitro Techniques; D010618:Phenanthrolines / Q000494:pharmacology
D002413:Cations, Divalent; D010618:Phenanthrolines; D004247:DNA; C046184:endodeoxyribonuclease PaeI; D015252:Deoxyribonucleases, Type II Site-Specific; C025205:1,10-phenanthroline
Rol' dvukhvalentnykh kationov v reaktsii éndonukleoliza DNK restriktazami.