[Isolation of intact mitochondria from the rat liver using vibration].
A method for isolating mitochondria from the rat liver is described. In this method the homogenization step is replaced by vibration with the frequency of 50 Hz realized by a simple device. Mitochondria isolated by vibration demonstrate higher indices of the oxidative phosphorylation with succinate and glutamate + malate used as substrates than those isolated by homogenization do. The method described permits decreasing considerably the isolation medium expenditure remaining the mitochondria yield per gram of the liver unchanged.
Petrenko
A Iu
AIu
Sukach
A N
AN
D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D008297:Male; D008930:Mitochondria, Liver / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D014732:Vibration
Vydelenie intaktnykh mitokhondriĭ iz pecheni krys s ispol'zovaniem vibratsii.