title: [Expression of human growth hormone in cultured mouse fibroblasts].
abstract: The new system for the transfer and expression of foreign genes based on retroviral vectors pPS-neo, conferring neomycin resistance was constructed. The BALB/c mouse cell lines producing highly active human growth hormone (more than 7 micrograms/ml into culture medium) were constructed using these vectors. An antibody column was used to purify the growth hormone from cell culture medium. Possibilities of producers to be applied for gene therapy are discussed.
authors.forename: M V
authors.initials: MV
authors.lastname: Reznikov
authors.forename: R
authors.initials: R
authors.lastname: Fidler
authors.forename: P M
authors.initials: PM
authors.lastname: Rubtsov
authors.forename: K G
authors.initials: KG
authors.lastname: Skriabin
authors.forename: P M
authors.initials: PM
authors.lastname: Chumakov
authors.forename: V S
authors.initials: VS
authors.lastname: Prasolov
authors.forename: A A
authors.initials: AA
authors.lastname: Baev
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism / Q000382:microbiology; D015870:Gene Expression; D005822:Genetic Vectors; D013006:Growth Hormone / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D011995:Recombination, Genetic; D012190:Retroviridae / Q000235:genetics
chemical_list: D013006:Growth Hormone
vernacular_title: Ekspressiia gormona rosta cheloveka v perevivaemykh fibroblastakh myshi.