Inhibition ELISA for hepatitis B surface antigen (HBsAg) using monoclonal idiotype-anti-idiotype interaction.
Monoclonal anti-idiotypic antibody (Anti-Id) to the common (a) epitope of hepatitis B surface antigen (HBsAg) were raised and used to detect serum HBsAg in an inhibition enzyme-linked immunosorbent assay (inhibition ELISA). HRP-labelled Id 8D-3-6 was reacted with Anti-Id 4-8H coated on the solid phase in the presence of HBsAg. The ability of the antigen to inhibit the binding of labelled Id 8D-3-6 to anti-Id 4-8H was determined and the results correlated well with those obtained by radioimmunoassay. This assay requires only one washing step, takes 2 h and covers the range 10 ng/ml to 1 microgram HBsAg/ml. The inhibition ELISA is a more convenient, rapid and relatively sensitive assay which can be used to measure the level of a wide range of serum HBsAg.
Kim
W B
WB
Department of Microbial Chemistry, College of Pharmacy, Seoul National University, Korea.
Jo
Y W
YW
Choi
E C
EC
Kim
B K
BK
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001667:Binding, Competitive; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006168:Guinea Pigs; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D006801:Humans; D007130:Immunoglobulin Idiotypes / Q000276:immunology; D051379:Mice; D011817:Rabbits; D011863:Radioimmunoassay
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D006514:Hepatitis B Surface Antigens; D007130:Immunoglobulin Idiotypes