title: The origin and fate of hyaluronan in amniotic fluid.
abstract: The mechanisms which regulate the steady-state concentration and molecular weight of hyaluronan in the amniotic fluid of sheep at different gestational ages have been investigated. An attempt to trace the origin of the polysaccharide has been made by analyses of various fetal fluids (amniotic fluid, allantoic fluid, tracheal fluid, urine, and serum). The fate has been studied by injection of radioactively labelled hyaluronan into the amniotic cavity and following the tracer in fetal tissues and fluids. The concentration of hyaluronan in amniotic fluid varies considerably but is in the order of 5 mg/l at mid-pregnancy and decreases to 1 mg/l in late pregnancy. The polysaccharide has a Mr-distribution with a weight-average in the order of 10(6) at 10 to 13 weeks of gestation which decreases to 10(5) closer to term. Calculations show that urine contributes 0.1 and 0.5 mg of low-molecular (Mr = 10(4) hyaluronan per day in mid- and late pregnancy, respectively, and the lung 10-20% of that amount in the form of high-molecular weight polymer (Mr greater than 10(6). The hyaluronan disappears from the amniotic cavity by bulk flow due to fetal swallowing. It is taken up and degraded in the fetal intestine. Molecules of Mr = 10(3) can pass the intestinal barrier. Calculations show that about 0.5 mg and 1.0 mg of hyaluronan is eliminated per day from the amniotic fluid at 12 and 17 weeks of gestation, respectively. Thus, the higher rate of elimination and the relatively high urinary contribution in more mature fetuses explain the low concentration and Mr of amniotic hyaluronan in late gestation, whereas a slower elimination combined with a relatively larger contribution of high molecular weight hyaluronan both from lung and urine and possibly from other sources are responsible for the higher concentration and Mr of the compound in early pregnancy.
authors.affiliation: Department of Medical and Physiological Chemistry, University of Uppsala, Sweden.
authors.forename: L B
authors.initials: LB
authors.lastname: Dahl
authors.forename: W G
authors.initials: WG
authors.lastname: Kimpton
authors.forename: R N
authors.initials: RN
authors.lastname: Cahill
authors.forename: T J
authors.initials: TJ
authors.lastname: Brown
authors.forename: R E
authors.initials: RE
authors.lastname: Fraser
mesh_terms: D000482:Allantois / Q000032:analysis; D000653:Amniotic Fluid / Q000378:metabolism*; D000818:Animals; D002845:Chromatography; D005260:Female; D005333:Fetus / Q000378:metabolism*; D006820:Hyaluronic Acid / Q000378:metabolism* / Q000493:pharmacokinetics; D008970:Molecular Weight; D011247:Pregnancy; D012756:Sheep; D014018:Tissue Distribution
chemical_list: D006820:Hyaluronic Acid