Intraocular PMMA lenses modified with surface-immobilized heparin: evaluation of biocompatibility in vitro and in vivo.
Intraocular lenses (IOL) were surface modified with covalently linked heparin. The surface-bound heparin could not be removed by incubation in solutions known to be effective in breaking non-covalent bonds, nor by incubation in a solution of proteinase K and only to a limited extent by incubation with heparinase. In vitro studies demonstrated improved biocompatibility by the heparin surface-modified lens with respect to outgrowth of fibroblasts and macrophages, activation of granulocytes and adhesion of platelets. These results were subsequently verified in vivo in terms of less inflammatory cells on the lens surface and fewer incidences of synechiae after 3 and 6 wk IOL implantation in the rabbit eye.
Larsson
R
R
Department of Biomedical Research, Pharmacia LEO Therapeutics AB, Uppsala, Sweden.
Selén
G
G
Björdklund
H
H
Fagerholm
P
P
D000818:Animals; D001672:Biocompatible Materials; D002448:Cell Adhesion / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects; D006098:Granulocytes / Q000187:drug effects; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007910:Lenses, Intraocular; D008264:Macrophages / Q000187:drug effects; D008768:Methylmethacrylates; D010973:Platelet Adhesiveness / Q000187:drug effects; D011474:Prosthesis Design; D011817:Rabbits; D013499:Surface Properties
D001672:Biocompatible Materials; D008768:Methylmethacrylates; D006493:Heparin