Effect of 1 alpha,25-dihydroxyvitamin D3 on polyamine metabolism in human monocyte cell line-U937.
1. Pretreatment with 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] caused an increase in ornithine decarboxylase (EC 4.1.1.17) (ODC) activity in lipopolysaccharide (LPS)-stimulated human monocyte cell line, U937. 2. The increase in ODC activity was dose-dependent and preincubation-time dependent. 3. 26,26,26,27,27,27-Hexafluoro-1,25-dihydroxyvitamin D3 was ca 7 times more potent than 1,25-(OH)2D3 in increasing ODC activity. 4. Pretreatment with 1,25-(OH)2D3 also potentiated the activity of spermidine/spermine-N1-acetyltransferase in LPS-stimulated U937 cells. 5. Putrescine levels in cells pretreated with 1,25-(OH)2D3 increased ca 2-fold 4-8 hr after LPS addition. 6. However, pretreatment with 1,25-(OH)2D3 did not cause any increase in ODC mRNA level, suggesting that 1,25-(OH)2D3 may modulate polyamine metabolism at the posttranscriptional level rather than the transcriptional step.
Yukioka
K
K
Second Department of Internal Medicine, Osaka City University Medical School, Japan.
Otani
S
S
Matsui-Yuasa
I
I
Goto
H
H
Tahara
H
H
Morisawa
S
S
Okuno
S
S
Nishizawa
Y
Y
Morii
H
H
D000123:Acetyltransferases / Q000378:metabolism; D002117:Calcitriol / Q000494:pharmacology*; D002460:Cell Line; D004357:Drug Synergism; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D009955:Ornithine Decarboxylase / Q000096:biosynthesis / Q000235:genetics; D011073:Polyamines / Q000378:metabolism*; D011700:Putrescine / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D000099228:Diamine N-Acetyltransferase
D000123:Acetyltransferases; D002117:Calcitriol; D008070:Lipopolysaccharides; D009955:Ornithine Decarboxylase; D011073:Polyamines; D011700:Putrescine; D012333:RNA, Messenger; D000099228:Diamine N-Acetyltransferase