title: Enzyme-linked immunosorbent assays for human tissue kallikrein and analysis of immunoreactive kallikrein in the plasma by them.
abstract: In order to study immunoreactive tissue kallikrein (TK) in human plasma, three kinds of enzyme-linked immunosorbent assay (ELISA) systems for human urinary kallikrein (HUK) were developed. All three types of TK in the plasma, i.e., active kallikrein, prokallikrein and kallikrein-alpha 1 antitrypsin (alpha 1 AT) complex, could be measured by the competitive ELISA (C-ELISA). However, the sandwich ELISA (S-ELISA) showed more strict specificity. Namely, both active and prokallikreins were able to be measured by the S-ELISA, while kallikrein-alpha 1 AT complex was hardly measurable. Measurement of this kallikrein-alpha 1 AT complex was made possible by using horseradish peroxidase (HRP)-labeled anti-alpha 1 AT IgG instead of HRP-labeled anti-HUK Fab' as the second antibody (HS-ELISA). Using these ELISAs we studied TK in plasma and the following observations were made. 1) The greater part of TK in the plasma was found in a form of a complex with alpha 1 AT. 2) The amount of free type TK was very small and the most part of this type TK in normal plasma was prokallikrein.
authors.affiliation: Department of Biochemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kizuki
authors.forename: S
authors.initials: S
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Aoki
authors.forename: M
authors.initials: M
authors.lastname: Kamada
authors.forename: M
authors.initials: M
authors.lastname: Ikekita
authors.forename: T
authors.initials: T
authors.lastname: Inaba
authors.forename: H
authors.initials: H
authors.lastname: Moriya
mesh_terms: D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G; D007610:Kallikreins / Q000032:analysis / Q000097:blood* / Q000302:isolation & purification; D007700:Kinetics; D011288:Prekallikrein / Q000032:analysis
chemical_list: D007074:Immunoglobulin G; D011288:Prekallikrein; D007610:Kallikreins