Comparative studies on an antigenicity of plasma proteins from humans and apes by ELISA: a close relationship of chimpanzee and human.
1. Antigenic differences between human and ape plasma proteins were quantitatively investigated by enzyme-linked immunosorbent assay (ELISA) using antisera against human and chimpanzee plasmas. 2. With anti-human plasma serum, both the chimpanzee and gorilla were very close to the human, although the chimpanzee was slightly closer to the human than to the gorilla; relative immunological distance (relative ID) of the chimpanzee was 71, while that of the gorilla was 74. 3. With anti-chimpanzee plasma serum, the chimpanzee was found to be closely related to the human; relative ID of the chimpanzee was 58, while that of the gorilla was 75. 4. From these a molecular phylogeny for humans and apes was deduced; among living apes, the chimpanzee is the most closely related species to the human.
Tsutsumi
H
H
Criminal Science Laboratory, Aichi Prefectural Police Headquarters, Nagoya, Japan.
Nakamura
S
S
Sato
K
K
Tamaki
K
K
Katsumata
Y
Y
D000818:Animals; D000941:Antigens; D001798:Blood Proteins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006071:Gorilla gorilla / Q000097:blood / Q000276:immunology; D015186:Hominidae / Q000097:blood* / Q000276:immunology; D006801:Humans; D007120:Immunochemistry; D002679:Pan troglodytes / Q000097:blood / Q000276:immunology; D010802:Phylogeny; D013045:Species Specificity
D000941:Antigens; D001798:Blood Proteins