Alteration of follicular steroid secretion and thecal morphology after in-vitro exposure of individual pig follicles to follicle regulatory protein.
Medium-sized (4-6 mm) pig follicles were incubated for 10 h and then examined via light microscopy. Treatment with pig FSH resulted in significantly increased concentrations of oestradiol, testosterone, androstenedione and progesterone in the medium. Follicle regulatory protein (FRP) alone (1 micrograms/ml) decreased follicular secretion of oestradiol (56%) and progesterone (53%) but stimulated the secretion of testosterone (226%) and androstenedione (139%). In the presence of 1 ng FSH/ml, the inhibitory effect of FRP on oestradiol secretion was enhanced (74%), progesterone values were unaffected and secretion of testosterone and androstenedione were reduced by 66% and 53%, respectively. All effects of FRP were fully overcome by 1 micrograms FSH/ml. The incidence of atresia, as defined by granulosa cell pycnosis, was similar in all treatment groups (1-3 of 10 follicles per group). The remaining follicles had intact granulosa cells. However, follicles treated with FRP (1 micrograms/ml) + FSH (1 ng/ml) had pycnotic nuclei in the theca interna cells, in the presence of an intact stratum granulosum. External exposure of follicles to FRP may not reflect physiological conditions since, in vivo, thecal pycnosis is never observed before granulosa cell pycnosis. However, the present results indicate that FRP is potentially capable of altering both follicular morphology and steroidogenesis. We suggest that FSH and FRP interact to affect follicular development.
Westhof
G
G
Livingston Reproductive Biology Laboratory, Department of Obstetrics and Gynecology, University of Southern California School of Medicine, Los Angeles 90033.
Westhof
K F
KF
Ahmad
N
N
diZerega
G S
GS
D000735:Androstenedione / Q000378:metabolism; D000818:Animals; D004958:Estradiol / Q000378:metabolism; D005260:Female; D012739:Gonadal Steroid Hormones / Q000378:metabolism*; D006131:Growth Inhibitors / Q000494:pharmacology*; D066298:In Vitro Techniques; D036341:Intercellular Signaling Peptides and Proteins; D006080:Ovarian Follicle / Q000033:anatomy & histology / Q000378:metabolism*; D010455:Peptides / Q000494:pharmacology*; D011374:Progesterone / Q000378:metabolism; D013552:Swine; D013739:Testosterone / Q000378:metabolism; D013799:Theca Cells / Q000187:drug effects*
D012739:Gonadal Steroid Hormones; D006131:Growth Inhibitors; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; C018355:intraovarian peptides; D013739:Testosterone; D000735:Androstenedione; D011374:Progesterone; D004958:Estradiol