Simultaneous identification of amphetamine and methamphetamine using solid-phase extraction and gas chromatography/nitrogen phosphorous detection or gas chromatography/mass spectrometry.
A method for the simultaneous detection and quantitation of amphetamine and methamphetamine in urine is described. Using solid-phase extraction, amphetamine, methamphetamine, and n-propylamphetamine (internal standard) are extracted from urine samples. Drugs in their free form are identified using gas chromatography/nitrogen-phosphorous detection (GC/NPD), whereas their heptafluorobutyric anhydride derivatives are detected by gas chromatography/mass spectrometry (GC/MS) in the selected ion monitoring (SIM) mode. Limits of detection for both amphetamine and methamphetamine are approximately 35 ng/mL. The procedure is simple, rapid, and suitable for a large number of specimens (25 or more).
Taylor
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Los Angeles County Sheriff's Department, Scientific Services Bureau, California 90057-2494.
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Philip
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Jain
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D000661:Amphetamine / Q000652:urine*; D005069:Evaluation Studies as Topic; D005410:Flame Ionization; D008401:Gas Chromatography-Mass Spectrometry; D008694:Methamphetamine / Q000652:urine*; D013048:Specimen Handling / Q000379:methods
D008694:Methamphetamine; D000661:Amphetamine