[The toxicity of sanguinarine compared to a number of other DNA-tropic compounds for ethidium bromide-sensitive and -resistant transformed murine fibroblasts in culture].
A natural DNA-intercalator plant benzo-c-phenanthridine alkaloid sanguinarine is more toxic for mouse transformed fibroblast L-cells in culture than synthetic DNA-intercalator ethidium bromide (EtB) and alkaloid berberine. Dimidium bromide is also an inhibitor of the L-cell growth. In assay conditions, growth of L-cells is stopped by 1.5 x 10(-5) M of sanguinarine. Lebr-625 cells, resistant to 25 micrograms/ml of EtB, have sanguinarine sensitivity close to that of L-cells, but Lebr-625 cells are resistant to dimidium bromide. Sanguinarine is more toxic for L-cells in culture than the anticancer drug cis-PtNH3)2Cl2. Trans-Pt(NH3)2Cl2 is less toxic for these cells. The strong toxicity of sanguinarine for L- and Lebr-625 cells in culture, as compared to other DNA-complexing drugs, seems to be associated with the wide range of potential cell targets for sanguinarine influence. Besides the inhibition of nucleic acid metabolism reactions, characteristic of DNA-intercalators, and disruption the mitochondrial ATP synthesis, also characteristic of organic heterocyclic cationic molecules of DNA-intercalators, sanguinarine can modify the thiol groups of enzymes including SH-sensitive membrane-bound Na+, K(+)-ATPase of cerebral cortex and Ca2(+)-ATPase of skeletal muscle sarcoplasmic reticulum fragments.
Beliaeva
T N
TN
Faddeeva
M D
MD
Sal'nikov
K V
KV
Ignatova
T N
TN
D000470:Alkaloids / Q000633:toxicity*; D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity; D053119:Benzophenanthridines; D001599:Berberine / Q000633:toxicity; D002472:Cell Transformation, Viral / Q000187:drug effects*; D002945:Cisplatin / Q000633:toxicity; D004247:DNA / Q000187:drug effects*; D004351:Drug Resistance; D004996:Ethidium / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects; D007364:Intercalating Agents / Q000633:toxicity*; D007546:Isoquinolines; D007739:L Cells / Q000187:drug effects; D051379:Mice; D010617:Phenanthridines / Q000633:toxicity
D000470:Alkaloids; D000970:Antineoplastic Agents; D053119:Benzophenanthridines; D007364:Intercalating Agents; D007546:Isoquinolines; D010617:Phenanthridines; D001599:Berberine; C035275:dimidium; D004247:DNA; C005705:sanguinarine; D004996:Ethidium; D002945:Cisplatin
Toksichnost' sangvinarina v sravnenii s riadom drugikh DNK-tropnykh soedineniĭ dlia chuvstvitel'nykh i rezistentnykh k bromistomu étidiiu transformirovannykh myshinykh fibroblastov v kul'ture.