title: Liposome-encapsulated hemoglobin as an artificial red blood cell: characterization and scale-up.
abstract: Encapsulation methods using high pressure extrusion and homogenization were developed which produce hemoglobin-containing liposomes, approximately one micron or less in diameter with an oxygen-carrying capacity of more than half that of red blood cells (RBCs). These methods were developed for scaling-up liposome-encapsulated hemoglobin (LEH) production. Previously, the lack of adequate scale-up methods has been a serious barrier to full scale efficacy and toxicity testing for all the researchers engaged in such investigations. The scale-up methods and characterization of the resulting LEH preparations are presented.
authors.affiliation: Department of Chemical Engineering, Illinois Institute of Technology, Chicago.
authors.forename: A
authors.initials: A
authors.lastname: Vidal-Naquet
authors.forename: J L
authors.initials: JL
authors.lastname: Gossage
authors.forename: T P
authors.initials: TP
authors.lastname: Sullivan
authors.forename: J W
authors.initials: JW
authors.lastname: Haynes
authors.forename: B H
authors.initials: BH
authors.lastname: Gilruth
authors.forename: R L
authors.initials: RL
authors.lastname: Beissinger
authors.forename: L R
authors.initials: LR
authors.lastname: Sehgal
authors.forename: A L
authors.initials: AL
authors.lastname: Rosen
mesh_terms: D001802:Blood Substitutes; D004337:Drug Carriers; D004339:Drug Compounding; D004912:Erythrocytes; D006454:Hemoglobins / Q000008:administration & dosage*; D006801:Humans; D008081:Liposomes; D010100:Oxygen / Q000378:metabolism; D010316:Particle Size; D010743:Phospholipids
chemical_list: D001802:Blood Substitutes; D004337:Drug Carriers; D006454:Hemoglobins; D008081:Liposomes; D010743:Phospholipids; D010100:Oxygen