title: Human cellular immune responses to Bordetella pertussis infection.
abstract: We have compared the responses of peripheral blood leucocytes from three groups (i) patients suffering from pertussis (whooping cough), (ii) clinical staff caring for those patients and laboratory staff working with Bordetella pertussis, and (iii) staff with no known recent contact with B. pertussis. In vitro stimulation with filamentous haemagglutinin (FHA) caused significant increases in proliferation of only the patient group's lymphocytes. In vitro stimulation with pertussis toxin (PT) caused a large increase in proliferation of lymphocytes from all three groups and in the patient group the increase in proliferation was related to the dose of PT. Interleukin 2 (IL-2) production by leucocytes from all three groups was significantly increased following challenge with FHA or PT. The increases in IL-2 production were greatest in lymphocytes from patients with pertussis. Challenge with toxoided pertussis toxin had no effect on either proliferation or IL-2 production in any of the groups.
authors.affiliation: National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, U.K.
authors.forename: A J
authors.initials: AJ
authors.lastname: Gearing
authors.forename: C R
authors.initials: CR
authors.lastname: Bird
authors.forename: K
authors.initials: K
authors.lastname: Redhead
authors.forename: M
authors.initials: M
authors.lastname: Thomas
mesh_terms: D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000032:analysis; D005260:Female; D006388:Hemagglutinins / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular; D007114:Immunization; D007223:Infant; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation; D008297:Male; D037342:Pertussis Toxin; D010566:Virulence Factors, Bordetella / Q000276:immunology; D014917:Whooping Cough / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D006388:Hemagglutinins; D007376:Interleukin-2; D010566:Virulence Factors, Bordetella; D037342:Pertussis Toxin