The cell barrier effect in rabbit corneas kept under eye bank conditions.
The influence of endothelial cell pumping and of cell barrier functions on cultivated corneas has been studied by comparing the rate of weight increase in freeze-treated, ouabain-treated and fresh corneas. Inhibition of the endothelial cell pump activity increased the swelling rate from 0.5% of start weight per h (fresh corneas) to about 2% per h (ouabain-treated corneas), as measured after 24 h in culture medium. The cell barrier effect was estimated by comparing the swelling rate of ouabain-treated corneas after 24 h, and the swelling rate of freeze-treated corneas with the same swelling pressure. The values are 2 and 9.5% per h, respectively. The swelling rate of freeze-treated corneas with different button diameters was found to increase with decreasing corneal button diameter.
Medin
W
W
University Eye Department, Rikshospitalet, University of Oslo, Norway.
Davanger
M
M
D000818:Animals; D003315:Cornea / Q000166:cytology* / Q000187:drug effects; D003470:Culture Media; D004728:Endothelium, Corneal / Q000166:cytology* / Q000187:drug effects; D005125:Eye Banks; D005615:Freezing; D010042:Ouabain / Q000494:pharmacology; D011817:Rabbits; D014015:Tissue Banks
D003470:Culture Media; D010042:Ouabain