Human chromosome banding with restriction endonucleases HaeIII, HinfI and PstI.
Human chromosome banding was carried out with restriction endonucleases (REs) HaeIII, HinfI, PstI and the effects of some factors on it were examined. HaeIII induced C and G banding patterns, HinfI induced negative C bands but the centromeric heterochromatin of chromosomes 3 and 4 remained selectively dark-stained. A new heteromorphism of C banded region was discovered in chromosome 4. PstI induced G-like banding pattern. The duration of enzymatic digestion, the concentration of glycerol in the reaction buffer, as well as the ageing and heating of chromosome preparations all had a significant influence on the banding effects of the REs.
Zhang
S Z
SZ
Department of Medical Genetics, West China University of Medical Sciences, Chengdu.
Dong
W F
WF
D002871:Chromosome Banding / Q000379:methods; D015252:Deoxyribonucleases, Type II Site-Specific; D006801:Humans; D007231:Infant, Newborn; D008677:Metaphase
C024388:CTGCAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; C032229:GANTC-specific type II deoxyribonucleases; C024403:GGCC-specific type II deoxyribonucleases