Liver uricase in Camelus dromedarius: purification and properties.
1. Uricase (urate: oxygen oxidoreductase, EC 1.7.3.3) was purified 750-fold from the liver of Camelus dromedarius. 2. The enzyme is a tetramer with a Mr of 100,000, displays high specificity for uric acid with a Km of 12 microM and is inhibited by a selected number of purine derivatives carrying oxygen at the C2 position. 3. The effect of pH and the inhibition by thiol compounds and chelating agents on the enzyme activity is reported. 4. Some lines of evidence suggesting the possibility of interaction of camel liver uricase with oligonucleotides are presented.
Osman
A M
AM
Department of Physiology, Faculty of Veterinary Medicine, National University of Somalia, Mogadishu.
Del Corso
A
A
Ipata
P L
PL
Mura
U
U
D000818:Animals; D002162:Camelus / Q000378:metabolism*; D002614:Chelating Agents / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D009696:Nucleic Acids / Q000378:metabolism; D009994:Osmolar Concentration; D013438:Sulfhydryl Compounds / Q000494:pharmacology; D014503:Urate Oxidase / Q000037:antagonists & inhibitors / Q000302:isolation & purification*
D002614:Chelating Agents; D009696:Nucleic Acids; D013438:Sulfhydryl Compounds; D014503:Urate Oxidase