title: Electrochemical detection of indole alkaloids of Catharanthus roseus in high-performance liquid chromatography.
abstract: Hydrodynamic voltammograms for indole and five indole alkaloids with different amino functions were obtained in order to evaluate the applicability of high-performance liquid chromatography (HPLC) with coulometric detection to these compounds. With the exception of serpentine, which has a quaternary nitrogen in its structure, all the compounds were oxidised and gave net signals of greater than 25 nA pmol(-1) at potentials of between +0.2 and +0.85 V versus a solid Pd electrode in an acetate-buffered (pH 6.5) water-methanol-acetonitrile system. An HPLC assay for quantifying picomole amounts of catharanthine and ajmalicine in Catharanthus roseus cell culture samples is described.
authors.forename: T
authors.initials: T
authors.lastname: Naaranlahti
authors.forename: V P
authors.initials: VP
authors.lastname: Ranta
authors.forename: P
authors.initials: P
authors.lastname: Jarho
authors.forename: M
authors.initials: M
authors.lastname: Nordström
authors.forename: S P
authors.initials: SP
authors.lastname: Lapinjoki
mesh_terms: D000470:Alkaloids / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D010944:Plants / Q000032:analysis*; D046948:Secologanin Tryptamine Alkaloids; D014748:Vinca Alkaloids / Q000032:analysis*; D015016:Yohimbine / Q000032:analysis*
chemical_list: D000470:Alkaloids; D046948:Secologanin Tryptamine Alkaloids; D014748:Vinca Alkaloids; D015016:Yohimbine; C005709:raubasine; C017836:catharanthine