Investigation of a persistently raised serum AST.
The AST responsible for a raised enzyme activity, with otherwise normal biochemistry, in a single individual over a 16-month period has been characterized as an IgG (lambda)-AST complex by (i) estimation of the molecular size by gel exclusion chromatography on Sephadex G200, (ii) agarose gel electrophoresis, (iii) immunoprecipitation by anti-immunoglobulin antisera and (iv) adsorption on to protein A-agarose.
Dawson
C M
CM
Department of Chemical Pathology, Norfolk and Norwich Hospital, UK.
Connelly
M S
MS
Kennedy
H J
HJ
Howe
G D
GD
Tickner
T R
TR
D000327:Adsorption; D000328:Adult; D001219:Aspartate Aminotransferases / Q000097:blood* / Q000276:immunology; D001797:Blood Protein Electrophoresis; D002850:Chromatography, Gel; D003600:Cytosol / Q000201:enzymology; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007527:Isoenzymes / Q000097:blood; D008928:Mitochondria / Q000201:enzymology; D013205:Staphylococcal Protein A
D007074:Immunoglobulin G; D007527:Isoenzymes; D013205:Staphylococcal Protein A; D001219:Aspartate Aminotransferases