The rapid and decremental change in haem oxygenase mRNA during erythroid differentiation of murine erythroleukaemia cells.
Changes in mRNA for haem oxygenase (HO), the rate-limiting enzyme for haem catabolism, were examined in murine Friend-virus transformed erythroleukaemia (MEL) cells while they were induced to undergo erythroid cell differentiation by treatment with dimethyl sulfoxide (DMSO). When MEL cells were treated with 1.5% (v/v) DMSO, a rapid decrease in HO mRNA content was observed (less than 12 h) which reached the lowest value at 18 h (18% of the untreated control). HO mRNA levels remained at substantially lower levels (approximately 50%) than those in untreated controls thereafter. A rapid decline in HO mRNA may be involved in the cellular events that determine the onset of erythroid differentiation.
Fujita
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Rockefeller University, New York, NY 10021.
Sassa
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D000818:Animals; D002454:Cell Differentiation; D002461:Cell Line, Transformed; D004121:Dimethyl Sulfoxide; D005622:Friend murine leukemia virus; D006419:Heme Oxygenase (Decyclizing) / Q000502:physiology*; D004915:Leukemia, Erythroblastic, Acute / Q000201:enzymology / Q000473:pathology*; D051379:Mice; D006899:Mixed Function Oxygenases / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis*
D012333:RNA, Messenger; D006899:Mixed Function Oxygenases; D006419:Heme Oxygenase (Decyclizing); D004121:Dimethyl Sulfoxide