title: Inhibition of hepatitis delta virus RNA replication in primary woodchuck hepatocytes.
abstract: Cell cultures of primary woodchuck hepatocytes can be infected with hepatitis delta virus (HDV) as demonstrated by the appearance of genomic HDV RNA 7 days after inoculation. This tissue culture system was used to study the effect of antiviral substances. Ribavirin inhibited HDV replication at a concentration of 10 micrograms/ml, if added up to three days post infection. Suramin had an inhibitory effect only when added simultaneously with the virus, at a concentration of 200 micrograms/ml. This concentration had no toxic effect on primary woodchuck hepatocytes. alpha-Amanitin showed a weak inhibitory effect only at the highest nontoxic concentration of 0.1 microgram/ml. Acyclovir had no effect.
authors.affiliation: Max von Pettenkofer Institute, University of Munich, F.R.G.
authors.forename: S S
authors.initials: SS
authors.lastname: Choi
authors.forename: R
authors.initials: R
authors.lastname: Rasshofer
authors.forename: M
authors.initials: M
authors.lastname: Roggendorf
mesh_terms: D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology; D000998:Antiviral Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D003698:Hepatitis Delta Virus / Q000235:genetics*; D008099:Liver; D008392:Marmota; D012367:RNA, Viral / Q000096:biosynthesis / Q000187:drug effects*; D013498:Suramin / Q000494:pharmacology
chemical_list: D000546:Amanitins; D000963:Antimetabolites; D000998:Antiviral Agents; D012367:RNA, Viral; D013498:Suramin