Inhibition of hepatitis delta virus RNA replication in primary woodchuck hepatocytes.
Cell cultures of primary woodchuck hepatocytes can be infected with hepatitis delta virus (HDV) as demonstrated by the appearance of genomic HDV RNA 7 days after inoculation. This tissue culture system was used to study the effect of antiviral substances. Ribavirin inhibited HDV replication at a concentration of 10 micrograms/ml, if added up to three days post infection. Suramin had an inhibitory effect only when added simultaneously with the virus, at a concentration of 200 micrograms/ml. This concentration had no toxic effect on primary woodchuck hepatocytes. alpha-Amanitin showed a weak inhibitory effect only at the highest nontoxic concentration of 0.1 microgram/ml. Acyclovir had no effect.
Choi
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Max von Pettenkofer Institute, University of Munich, F.R.G.
Rasshofer
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Roggendorf
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D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology; D000998:Antiviral Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D003698:Hepatitis Delta Virus / Q000235:genetics*; D008099:Liver; D008392:Marmota; D012367:RNA, Viral / Q000096:biosynthesis / Q000187:drug effects*; D013498:Suramin / Q000494:pharmacology
D000546:Amanitins; D000963:Antimetabolites; D000998:Antiviral Agents; D012367:RNA, Viral; D013498:Suramin