Polypeptide composition of normal and neoplastic human breast tissues and cells analyzed by two-dimensional gel electrophoresis.
The protein populations of epithelial cells cultured from two neoplastic and five non-neoplastic human breast tissues were resolved and displayed by two-dimensional polyacrylamide gel electrophoresis and silver-staining. With a computer-based image analysis system, we identified eight polypeptides which are present in both of the neoplastic cell lines, but absent from all five of the cultures of non-neoplastic breast cells. The eight polypeptides are not unique to cells cultured from neoplastic breast, because they are also found in cells cultured from non-breast tissues, both neoplastic and non-neoplastic. Two of the eight polypeptides (approximately Mr 25,000/pI 4.4 and approximately Mr 31,000/pI 5.5) are present in the patterns of whole tissue samples from infiltrating ductal carcinomas and absent in most normal breast tissue.
Maloney
T M
TM
Department of Research Administration, St. John's University, Jamaica, New York 11439.
Paine
P L
PL
Russo
J
J
D001940:Breast / Q000473:pathology; D001943:Breast Neoplasms / Q000032:analysis* / Q000473:pathology / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D010455:Peptides / Q000032:analysis*; D014407:Tumor Cells, Cultured / Q000032:analysis*
D010455:Peptides