title: Isolation and characterization of genes coding for proteins involved in the cytolysis by Listeria ivanovii.
abstract: We established a library of chromosomal DNA of Listeria ivanovii in the pTZ19R plasmid system, using Escherichia coli DH5 alpha as the host. One recombinant clone reacted strongly with a polyclonal antiserum raised against the listeriolysin O and a second exoprotein (24kDa) of L. ivanovii, which is most probably also involved in cytolytic processes. The recombinant E. coli clone may contain part of the listeriolysin O gene of L. ivanovii.
authors.affiliation: Institute of Genetics and Microbiology, University of Würzburg, FRG.
authors.forename: A
authors.initials: A
authors.lastname: Haas
authors.forename: J
authors.initials: J
authors.lastname: Kreft
authors.forename: W
authors.initials: W
authors.lastname: Goebel
mesh_terms: D015153:Blotting, Western; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D015723:Gene Library; D005798:Genes, Bacterial; D006460:Hemolysin Proteins; D006461:Hemolysis / Q000235:genetics*; D006801:Humans; D008087:Listeria / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D006460:Hemolysin Proteins