[Use of spin probe in the determination of oxygen consumption in human neutrophils].
A quantitative method of the oxygen consumption rate measurement by human blood neutrophils upon their activation is described. This method is based on the spin exchange determination between 3-carbamoyl-2,2,5,5-tetramethyl-3-pyrroline-1-yloxyl spin probe and oxygen molecules from the ESR spectra. Method allows the determination of about 1 micromolar concentrations of oxygen.
Osipov
A N
AN
Kulikov
O A
OA
Azizova
O A
OA
D003497:Cyclic N-Oxides; D006801:Humans; D009504:Neutrophils / Q000032:analysis* / Q000378:metabolism; D010100:Oxygen / Q000097:blood; D010101:Oxygen Consumption; D013113:Spin Labels
D003497:Cyclic N-Oxides; D013113:Spin Labels; C047253:3-carbamoyl-2,2,5,5-tetramethyl-3-pyrroline-1-yloxyl; D010100:Oxygen
Ispol'zovanie spinovogo zonda dlia izmereniia potrebleniia kisloroda neĭtrofilami cheloveka.