title: Preparation of protoplasts of Ustilago violacea for transformation and pulse-field electrophoresis.
authors.affiliation: Dept. of Biology, University of Louisville, KY 40292.
authors.forename: A K
authors.initials: AK
authors.lastname: Bej
authors.forename: M H
authors.initials: MH
authors.lastname: Perlin
mesh_terms: D001487:Basidiomycota / Q000166:cytology*; D002458:Cell Fractionation / Q000379:methods*; D002480:Cellulase; D004587:Electrophoresis, Agar Gel; D004798:Enzymes; D005966:Glucuronidase; D008278:Magnesium Sulfate; D011523:Protoplasts; D014170:Transformation, Genetic; D014588:Ustilago / Q000166:cytology*
chemical_list: D004798:Enzymes; C045154:novozym 234; D008278:Magnesium Sulfate; D005966:Glucuronidase; D002480:Cellulase