Determination of G + C content of DNA using high-performance liquid chromatography for the identification of staphylococci and micrococci.
The guanine-plus-cytosine (G + C) content of different species of Staphylococcus and Micrococcus was determined by high-performance liquid chromatography. Purified bacterial DNA was hydrolysed by nuclease P1. The nucleotides were separated by chromatography and quantified by measurement of the optical density at 260 nm. The G + C content of staphylococci ranged from 31.5 to 37.9 moles %, and that of micrococci from 68.7 to 75.2. Most of our results were comparable to those obtained with the thermal method.
Peyret
M
M
Centre National de Référence des Staphylocoques, Laboratoire de Bactériologie, Faculté de Médecine Alexis Carrel, Lyon, France.
Freney
J
J
Meugnier
H
H
Fleurette
J
J
D002851:Chromatography, High Pressure Liquid; D003596:Cytosine / Q000032:analysis; D004269:DNA, Bacterial / Q000032:analysis*; D006147:Guanine / Q000032:analysis; D008837:Micrococcus / Q000032:analysis / Q000145:classification*; D013210:Staphylococcus / Q000032:analysis / Q000145:classification*
D004269:DNA, Bacterial; D006147:Guanine; D003596:Cytosine