title: Bacterial contamination of intraocular and extraocular fluids during extracapsular cataract extraction.
abstract: Fluid aspirated from the anterior chamber and fluid drained from the conjunctival sac during 101 extracapsular cataract operations was examined for bacterial contamination. Bacteria were grown by enrichment culture from the conjunctival sac of 90 eyes and from the anterior chamber aspirate of 29 eyes. Conjunctival fluid, stained with fluorescein, was demonstrated to flow into the anterior chamber during the aspiration stage of extracapsular cataract extraction and during intraocular lens implantation. It is suggested that this fluid from the conjunctival sac, contaminated with bacteria, routinely enters the anterior chamber during extracapsular cataract extraction and is the likely source of some cases of post-operative endophthalmitis.
authors.affiliation: West of England Eye Infirmary, Exeter.
authors.forename: D R
authors.initials: DR
authors.lastname: Sherwood
authors.forename: W J
authors.initials: WJ
authors.lastname: Rich
authors.forename: J S
authors.initials: JS
authors.lastname: Jacob
authors.forename: R J
authors.initials: RJ
authors.lastname: Hart
authors.forename: Y L
authors.initials: YL
authors.lastname: Fairchild
mesh_terms: D000867:Anterior Chamber / Q000382:microbiology*; D001082:Aqueous Humor / Q000382:microbiology*; D001419:Bacteria / Q000302:isolation & purification; D002387:Cataract Extraction / Q000009:adverse effects*; D003228:Conjunctiva / Q000382:microbiology*; D009877:Endophthalmitis / Q000209:etiology*; D015818:Eye Infections, Bacterial / Q000209:etiology*; D019793:Fluorescein; D005452:Fluoresceins; D006801:Humans
chemical_list: D005452:Fluoresceins; D019793:Fluorescein