[Distribution and biotransformation of labelled pantothenate in cytoplasmic and mitochondrial fractions of the rat liver with a deficiency of pantothenic acid].
Experiments were conducted on white rats given synthetic rations devoid of pantothenic acid during 10 weeks. Intensification of 14C-pantothenate deposition was recorded 30 min and 4 h after its intraperitoneal administration. The mitochondrial fraction of the liver accumulated the isotope in time. High-performance liquid chromatography used for separation of the vitamin labeled metabolites has revealed phosphopantothenate (pantothenate), phosphopantothein, CoA and dephospho-CoA (pantetein) in the liver homogenate, while in the mitochondria extracts only CoA and dephospho-CoA (pantetein) were detected. It has been suggested that dephosphorylation of pantothenate metabolites and rapid transformation of phosphopantetein into CoA may take place during the separation of the fractions.
Reziapkin
V I
VI
Gurinovich
V A
VA
Chiger
V B
VB
Moiseenok
A G
AG
D000818:Animals; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D003065:Coenzyme A / Q000032:analysis; D003593:Cytoplasm / Q000201:enzymology / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000201:enzymology / Q000378:metabolism*; D010205:Pantothenic Acid / Q000172:deficiency / Q000493:pharmacokinetics*; D051381:Rats
D010205:Pantothenic Acid; D003065:Coenzyme A
Raspredelenie i biotransformatsiia mechenogo pantotenata v tsitoplazmaticheskoĭ i mitokhondrial'noĭ fraktsii pecheni krys s nedostatochnost'iu pantotenovoĭ kisloty.