Morphology and fertilizability of zona-free hamster eggs separated into halves and quarters by centrifugation.
When mature hamster eggs were freed from their zonae pellucidae and centrifuged in a Percoll gradient, each egg was separated into a light half and a heavy half. Chromosomes remained in their original position during centrifugation, resulting in the production of light and heavy halves with and without chromosomes. When the eggs were treated with cytochalasin D (CD) and then centrifuged, the chromosomes moved to the centripetal pole and were extruded rapidly before each egg separated into halves or fragments. In the eggs without CD treatment, the density of cortical granules was reduced in the centripetal region of the egg. In those treated with CD, the density of the granules was reduced in both centripetal and centrifugal regions of the egg. Both light and heavy halves were fertilizable. There was, however, a notable difference between light and heavy halves. Most of the heavy halves supported development of sperm nuclei into pronuclei, whereas only few of light halves could do so, suggesting that most of light halves were lacking or deficient in materials necessary for the development of a sperm (male) pronucleus. When the light and heavy halves were centrifuged further, each separated into two quarters. The lightest quarter, which was almost totally devoid of organelles, was buoyant and very fragile. Spermatozoa could fuse with it, but the incidence of the fusion was low. In this quarter, the sperm nucleus could decondense, but could not develop into a pronucleus. This was in marked contrast with other three quarters in which sperm nuclei could develop into well-formed pronuclei.
Yang
C H
CH
Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Honolulu 96822.
Yanagimachi
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R
Yanagimachi
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H
D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002499:Centrifugation, Density Gradient; D002875:Chromosomes; D006224:Cricetinae; D015638:Cytochalasin D; D003594:Cytoplasmic Granules / Q000187:drug effects; D005260:Female; D005306:Fertilization / Q000502:physiology*; D008677:Metaphase; D010063:Ovum / Q000166:cytology / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure*; D015044:Zona Pellucida / Q000502:physiology*
D015638:Cytochalasin D