Serum complement activity and serum enzymes in rats after a subcutaneous injection of toxin prepared from Pasteurella multocida type D.
Toxin produced by Pasteurella multocida type D was investigated for its effect on serum complement and serum biochemistry in rats. Rats were given a sublethal single subcutaneous injection of D toxin equivalent to 0.2 microgram/kg of body weight. Serum obtained 1, 3, 5 and 7 days post-treatment was tested for complement activity, total bilirubin, aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP). Serum complement titers were significantly elevated (P less than 0.05) at all times after injection of toxin compared to rats injected with diluent and tested at the same intervals. Bilirubin was decreased but both control and D toxin-treated rats had low concentrations of bilirubin in their sera. The other biochemical constituents measured had no consistent pattern that would indicate liver damage in the rats.
Thurston
J R
JR
USDA, Agricultural Research Service, National Animal Disease Center, MWA, Ames, IA 50010.
Cheville
N F
NF
Rimler
R B
RB
Sacks
J
J
D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000302:isolation & purification / Q000633:toxicity*; D001663:Bilirubin / Q000097:blood; D003165:Complement System Proteins / Q000378:metabolism*; D004798:Enzymes / Q000097:blood*; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D010325:Pasteurella; D051381:Rats
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004798:Enzymes; C062258:Pasteurella multocida toxin; D003165:Complement System Proteins; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D001663:Bilirubin