title: A petite positive strain of Kluyveromyces lactis has a 300 kb deletion in the rDNA cluster.
abstract: By employing Pulsed Field Gel Electrophoresis we have shown that the chromosomal DNA pattern of a petite-positive strain of Kluyveromyces lactis, designated KF4, differs from the karyotype of the reference strain. Digestion with SfiI and hybridization with an rDNA probe, demonstrate that a novel chromosomal band in KF4 results from a deletion of 35-40 rDNA cistrons from the rDNA cluster. The significance of this finding to possible alterations in cell physiology and the ability to generate "petite" mutants is discussed.
authors.affiliation: Molecular and Population Genetics Group, Research School of Biological Sciences, Australian National University, Canberra, ACT.
authors.forename: R
authors.initials: R
authors.lastname: Maleszka
authors.forename: G D
authors.initials: GD
authors.lastname: Clark-Walker
mesh_terms: D015139:Blotting, Southern; D002872:Chromosome Deletion; D004271:DNA, Fungal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics*; D007621:Karyotyping; D007716:Kluyveromyces / Q000235:genetics*; D010641:Phenotype; D012091:Repetitive Sequences, Nucleic Acid; D004718:Saccharomycetales / Q000235:genetics*
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal