Intermediate filament proteins immunologically related to desmin in astrocytes: a study of chicken spinal cord by two-dimensional gel electrophoresis and immunoblotting.
Co-migration experiments by two-dimensional SDS-PAGE using chicken spinal cord extracts and desmin purified from chicken gizzard showed that desmin is not present in spinal cord. However, by the immunoblotting procedure, desmin antibodies recognized 3 spinal cord antigens with different molecular weights and isoelectric points than desmin and the glial fibrillary acidic (GFA) protein. These antigens which also reacted with GFA protein antibodies were not identified in chicken gizzard extracts. The reactivity of the antigens with a monoclonal antibody recognizing an epitope common to most intermediate filament proteins (1) suggests that immunostaining of astrocytes with desmin antibodies (2, 3) is due to the presence of new intermediate filament proteins immunologically related to desmin.
Dahl
D
D
Spinal Cord Injury Research Laboratory, Veterans Administration Medical Center, Boston, MA 02132.
Crosby
C J
CJ
Bignami
A
A
D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D002642:Chick Embryo; D003893:Desmin / Q000378:metabolism*; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D007525:Isoelectric Focusing; D008970:Molecular Weight; D013116:Spinal Cord / Q000378:metabolism*
D003893:Desmin; D007381:Intermediate Filament Proteins