title: An adaptor strategy to subclone entire cDNA libraries as single insert recombinants.
abstract: The current methods for subcloning entire cDNA libraries usually result in a significant portion of recombinants containing multiple inserts, since in most instances the inserts derived from the library to be subcloned are released as a bulk of self-ligatable DNA fragments. By use of an adaptor strategy, a method is presented to confer noncompatible ends to primarily self-ligatable inserts, resulting in efficient subcloning of entire libraries as single insert recombinants.
authors.affiliation: Medizinische Hochschule Hannover, Institut für Medizinische, Mikrobiologie, FRG.
authors.forename: E C
authors.initials: EC
authors.lastname: Böttger
mesh_terms: D001483:Base Sequence; D001709:Biotechnology; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000235:genetics*; D005822:Genetic Vectors; D015894:Genome, Human; D006801:Humans; D008969:Molecular Sequence Data; D011995:Recombination, Genetic
chemical_list: D004247:DNA