Detection and separation of intracellular 1-beta-D-arabinofuranosylcytosine-5-triphosphate by ion-pair high-performance liquid chromatography.
An ion-pair high-performance liquid chromatographic method, using a reversed-phase C18 column, was developed to provide an isocratic, sensitive, fast and reproducible separation of intracellular 1-beta-D-arabinofuranosylcytosine-5-triphosphate and its measurement at a low limit of 5 pmol by ultraviolet absorbance at 280 nm with a coefficient of variation lower than 10%. A rapid separation is achieved by using a backflush procedure at 16 min and the retention time is 14 min.
Schleyer
E
E
Department of Internal Medicine, University of Münster, F.R.G.
Ehninger
G
G
Zühlsdorf
M
M
Proksch
B
B
Hiddemann
W
W
D001085:Arabinofuranosylcytosine Triphosphate / Q000032:analysis* / Q000493:pharmacokinetics; D001088:Arabinonucleotides / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007938:Leukemia / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D014407:Tumor Cells, Cultured
D001088:Arabinonucleotides; D001085:Arabinofuranosylcytosine Triphosphate