[Methodologic studies of the isolation of VLDL using the lipoprotein precipitation reaction in the preparation of apolipoprotein E (Apo E)].
Several lipoprotein precipitation reactions were examined in the isolation of VLDL for apo E phenotyping in comparison to ultracentrifugation. MgCl2-Heparin- and phosphotungstic acid precipitation revealed the best results, although an apo E phenotyping was possible in only 40% of the samples. Ultracentrifugation was unequivocally superior to lipoprotein precipitation reactions.
Gromoll
B
B
Krantz
S
S
D001057:Apolipoproteins E / Q000032:analysis*; D011232:Chemical Precipitation; D006801:Humans; D008079:Lipoproteins, VLDL / Q000302:isolation & purification*; D014461:Ultracentrifugation
D001057:Apolipoproteins E; D008079:Lipoproteins, VLDL
Methodische Untersuchungen zur Isolierung von VLDL mit Hilfe von Lipoprotein-Fällungsreaktionen bei der Darstellung von Apolipoprotein E (Apo E).