Isolation and characterization of mouse nasal lymphocytes.
A method for isolation of mouse nasal lymphocytes is described. Lymphocyte-enriched suspensions are examined for their morphologic, surface immune staining and mitogenic characteristics. This method will allow testing of immune function in the upper respiratory tract of the mouse.
Reuman
P D
PD
James N. Gamble Institute of Medical Research, Cincinnati, OH 45219.
Keely
S P
SP
Schiff
G M
GM
D000818:Animals; D002469:Cell Separation / Q000379:methods; D002499:Centrifugation, Density Gradient; D008168:Lung / Q000166:cytology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009297:Nasal Mucosa / Q000166:cytology*; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D013154:Spleen / Q000166:cytology
D011947:Receptors, Antigen, B-Cell