title: Genomic organisation of nuclear tRNAGly and tRNALeu genes in Trypanosoma brucei.
abstract: We have isolated a 0.3-kb HaeIII restriction fragment from Trypanosoma brucei which contains two tRNA genes. Secondary structure models predict that the two genes identified encode tRNA molecules which specify glycine (anticodon UCC) and leucine (anticodon CAG). The two genes are separated by 86 nucleotides, transcribed in the same direction and contain features of conventional RNA polymerase III transcription units. Southern blot analysis indicates the presence of multicopy tRNa gene families in T. brucei.
authors.affiliation: Department of Microbiology and Immunology, UCLA School of Medicine 90024.
authors.forename: D A
authors.initials: DA
authors.lastname: Campbell
authors.forename: Y
authors.initials: Y
authors.lastname: Suyama
authors.forename: L
authors.initials: L
authors.lastname: Simpson
mesh_terms: D000818:Animals; D001483:Base Sequence; D005796:Genes; D008928:Mitochondria / Q000378:metabolism; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D012345:RNA, Transfer, Amino Acid-Specific / Q000235:genetics*; D012353:RNA, Transfer, Gly / Q000235:genetics*; D012356:RNA, Transfer, Leu / Q000235:genetics*; D014346:Trypanosoma brucei brucei / Q000235:genetics*
chemical_list: D012345:RNA, Transfer, Amino Acid-Specific; D012353:RNA, Transfer, Gly; D012356:RNA, Transfer, Leu