[Arterial thrombosis--an experimental approach].
In this study description is given of a method developed in our laboratory for the investigation of platelet-vessel wall interaction and in vivo thrombus formation. The development of this method is based on the recording of the optical density of an arteriole. Following a standardized challenge by an electrical current onto the arterial wall and subsequent topical superfusion by adenosine diphosphate (ADP), endothelial cells demonstrate specific lesions and platelets start to adhere onto the site of lesion, they aggregate and a platelet thrombus develops. Using an optoelectronic device, which functions as an analogue computer, the generation and the growth of the thrombus in the arteriole can be followed and recorded over time. This continuous on-line recording of the thrombotic phenomenon made it possible to develop discriminating parameters. As such it was possible to study thromboformation in relation to the effect of the metabolites of the arachidonate cascade. Because it is possible to register the thrombotic phenomenon over time, ultrastructural analysis using transmission and scanning electronmicroscopy was performed and allowed us to investigate the interaction of the platelets, the involvement of leukocytes and the onset of fibrin deposition in the thrombus. In further investigations it was demonstrated that as a result of an appropriate challenge of the arterial wall, Paf-acether was generated within the arterial endothelial cells, inducing local thromboformation which perpetuated itself. As such, it could be demonstrated that as a result of the local generation of endogenous Paf-acether thromboformation by an inflammatory process was maintained, leading eventually to complete obstruction of the artery.
Bourgain
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RH
D000244:Adenosine Diphosphate / Q000008:administration & dosage; D000818:Animals; D001158:Arteries; D001160:Arterioles; D004556:Electric Injuries; D004730:Endothelium, Vascular; D008854:Microscopy, Electron / Q000379:methods; D010974:Platelet Aggregation; D051381:Rats; D011919:Rats, Inbred Strains; D013927:Thrombosis / Q000209:etiology / Q000473:pathology / Q000503:physiopathology*
D000244:Adenosine Diphosphate
Arteriële trombose--een experimentele benadering.