Induction of polyclonal and monoclonal antibodies with a single allergen separated from birch pollen extract by SDS-PAGE and nitrocellulose blots.
A Gold staining allergenic band, Bet v 1, was excised from a nitrocellulose blot following SDS-PAGE of birch pollen extract. This was used to raise a polyclonal rabbit antiserum and monoclonal mouse antibodies specific only for the Bet v 1. The method offers practical advantages for the production of antibodies to individual allergens in complex extract mixtures, without laborious purification methods.
Wheeler
A W
AW
Beecham Pharmaceuticals (Bencard), Epsom, UK.
Deards
M
M
Fox
B
B
Hickman
B E
BE
Spackman
V M
VM
D000485:Allergens / Q000276:immunology* / Q000302:isolation & purification; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D007123:Immunoelectrophoresis, Two-Dimensional; D007526:Isoelectric Point; D051379:Mice; D011058:Pollen / Q000276:immunology*; D011817:Rabbits; D014197:Trees
D000485:Allergens; D000911:Antibodies, Monoclonal