Study of bilirubin binding in human serum by high-performance liquid chromatography.
We describe a high-performance single-run liquid chromatographic method for the separation of protein-bound and "free" (loosely bound) bilirubin in human serum samples. The procedure is based on both the size-exclusion and the adsorptive properties of the column packing material, LiChrosorb Diol. The elution of all sample constituents is accomplished by means of an aqueous mobile phase containing a phosphate-buffered solution of human serum albumin or adult serum, supplemented by a small amount of bilirubin. The efficacy of the developed method is exemplified by assaying the bilirubin binding to human adult and infant sera.
Soltés
L
L
Ustav experimentálnej farmakológie, Centrum fyziologických vied, Slovenská akadémia vied, Bratislava, Ceskoslovensko.
Sébille
B
B
Tillement
J P
JP
Berek
D
D
D000328:Adult; D001663:Bilirubin / Q000097:blood / Q000378:metabolism*; D001798:Blood Proteins / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007223:Infant
D001798:Blood Proteins; D001663:Bilirubin