Gas chromatographic-mass spectrometric analysis of plasma nifedipine.
A gas chromatographic-mass spectrometric assay is described for the determination of plasma nifedipine using nitrendipine as an internal standard. Chromatographic separation was performed on a dimethylsilicone capillary column, and detection was by selected ion monitoring of electron impact-generated base peak ions. The lower limit of quantifiable detection of nifedipine was 2 ng/ml. The method was applied to plasma samples obtained from a human subject who had been dosed with a 10-mg nifedipine capsule every 8 h for eight doses.
Patrick
K S
KS
Department of Medicinal Chemistry, College of Pharmacy, University of Tennessee, Memphis 38163.
Jarvi
E J
EJ
Straughn
A B
AB
Meyer
M C
MC
D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008297:Male; D009543:Nifedipine / Q000097:blood* / Q000493:pharmacokinetics; D009568:Nitrendipine / Q000097:blood; D012015:Reference Standards
D009568:Nitrendipine; D009543:Nifedipine