Gangliosides and sulphatide in human cerebrospinal fluid: quantitation with immunoaffinity techniques.
A sensitive micromethod involving extraction, purification and thin-layer chromatography (TLC)-enzyme immunostaining was developed for the quantation of gangliosides and sulphatide, as markers for neuronal disorders and myelin disturbances, in individual samples of less than 5 ml of cerebrospinal fluid. The gangliosides of the gangliotetraose series were individually determined with cholera toxin subunit B by TLC-enzyme-linked immunosorbent assay (ELISA) after chromatography and subsequent sialidase hydrolysis to II3NeuAc-GgOse4Cer (GM1). Other gangliosides and sulphatide were determined with specific monoclonal antibodies by TLC-ELISA. The total ganglioside content varied between 100 and 230 nmol/l in ten normal cerebrospinal fluid samples from adults. The major gangliosides were of the gangliotetraose series, represented by GM1, IV3NeuAc,II3NeuAc-GgOse4Cer, (GD1a), II3(NeuAc)2-GgOse4Cer (GD1b) and IV3NeuAc,II3 (NeuAc)2-GgOse4Cer (GT1b) of which the b-series gangliosides dominated, i.e., GD1b and GT1b.
Davidsson
P
P
Department of Neurochemistry and Psychiatry, Göteborg University, St. Jörgen Hospital, Hisings Backa, Sweden.
Fredman
P
P
Svennerholm
L
L
D000328:Adult; D000911:Antibodies, Monoclonal; D002846:Chromatography, Affinity; D002855:Chromatography, Thin Layer; D004797:Enzyme-Linked Immunosorbent Assay; D005732:Gangliosides / Q000134:cerebrospinal fluid*; D006801:Humans; D013433:Sulfoglycosphingolipids / Q000134:cerebrospinal fluid*
D000911:Antibodies, Monoclonal; D005732:Gangliosides; D013433:Sulfoglycosphingolipids