A somatic cell hybrid mapping panel for regional assignment of human chromosome 13 DNA sequences.
We have constructed somatic cell hybrids containing different overlapping deletions involving human chromosome 13. Cytogenetic characterisation of the breakpoints allowed division of the chromosome into six distinct regions. Molecular characterisation of these hybrids allowed regional assignment of anonymous DNA sequences, cDNAs, and isoenzyme variants and these hybrids should prove valuable in the analysis and isolation of genes and disease loci on chromosome 13.
Cowell
J K
JK
Department of Haematology and Oncology, Institute of Child Health, London, UK.
Mitchell
C D
CD
D000818:Animals; D015139:Blotting, Southern; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000235:genetics; D002871:Chromosome Banding; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002882:Chromosomes, Human, Pair 13; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D015342:DNA Probes; D006801:Humans; D006822:Hybrid Cells; D007621:Karyotyping; D014178:Translocation, Genetic
D015342:DNA Probes; D004247:DNA; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; C020118:ESD protein, human