Chromatography of peptides on a multi-coil counter-current chromatograph.
A modified horizontal flow-through coil planet centrifuge has been constructed that has the potential for preparative chromatography. The multi-coil counter-current chromatograph, equipped with a set of four multi-layer coils, has have improved performance with polar solvents suitable for peptide elution. Separations of a group of dipeptides and purification of a cholecystokinin fragment and an undecapeptide were achieved with n-butanol-acetic acid-water at high flow-rates with good resolution.
Knight
M
M
Peptide Technologies Corporation, Washington, DC 20017-1004.
Ito
Y
Y
D002766:Cholecystokinin / Q000302:isolation & purification; D002845:Chromatography / Q000379:methods*; D004151:Dipeptides / Q000302:isolation & purification; D010455:Peptides / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
D004151:Dipeptides; D010455:Peptides; D002766:Cholecystokinin