[Heterogeneity of current epidemic influenza A viruses with respect to reproduction activity in a tissue culture].
The reproduction activity in human embryo kidney cell culture (HEK) of epidemic influenza A viruses (H3N2 serosubtype) isolated in different years and with a certain level of virulence for man was studied. The cells were inoculated with the viruses at a multiplicity of infection of 0.001 EID50/cell, and the reproductive activity was judged by the levels of infectious and hemagglutinating activity during 96 hours of observation. There was a clear-cut correlation between the virus virulence level and the reproductive activity in HEK cell culture. Epidemic influenza A (H1N1) viruses of the A/Chile/1/83 variant were found to be heterogeneous by the marker of their reproductive activity in HEK cells. It is presumed that the observed differences in the infectious activity of the viruses of this group are also associated with different levels of their virulence for man.
Lavrent'eva
I N
IN
Medvedeva
T E
TE
Golubev
D B
DB
D046508:Culture Techniques; D006389:Hemagglutinins, Viral / Q000032:analysis; D006801:Humans; D009980:Influenza A virus / Q000276:immunology / Q000472:pathogenicity* / Q000502:physiology; D014774:Virulence; D014779:Virus Replication
D006389:Hemagglutinins, Viral
Geterogennost' sovremennykh épidemicheskikh virusov grippa A po priznaku reproduktsionnoĭ aktivnosti v tkanevoĭ kul'ture.