The maternal store of the xlgv7 mRNA in full-grown oocytes is not required for normal development in Xenopus.
We have attempted to analyze the function of a maternal mRNA xlgv7 which is distributed as an animal-vegetal gradient in stage 6 oocytes using a combination of antisense oligodeoxynucleotide injection into oocytes followed by in vitro maturation and fertilization. Injection of 20 ng of the antisense oligodeoxynucleotide resulted in the destruction of the xlgv7 mRNA to undetectable levels. Upon maturation and fertilization the resulting embryos develop with no specific defects suggesting that the maternal store of xlgv7 in stage 6 oocytes is not required and that the embryo can develop solely with the maternal store of the xlgv7 protein. Also, these results demonstrate the feasibility of this approach in destroying a specific maternal RNA and assaying its effect on development.
Kloc
M
M
Department of Molecular Genetics, University of Texas MD Anderson Cancer Center, Houston 77030.
Miller
M
M
Carrasco
A E
AE
Eastman
E
E
Etkin
L
L
D000818:Animals; D009865:Oocytes / Q000502:physiology*; D012333:RNA, Messenger / Q000502:physiology*; D014982:Xenopus laevis / Q000196:embryology*
D012333:RNA, Messenger