Localization of 5-HT3 receptor binding sites in human dorsal vagal complex.
Binding of the 5-HT3 receptor ligand [3H]BRL 43694 was investigated in the human medulla oblongata using in vitro autoradiography. High levels of saturable, displaceable binding (Bmax 1.88 pmol/mg protein, Kd 1.21 nM) were seen in the dorsal vagal complex but in no other medullary region. The results provide evidence for the existence of 5-HT3 receptor binding sites in a brain region involved in the control of vomiting in man.
Reynolds
D J
DJ
M.R.C. Clinical Pharmacology Unit, Radcliffe Infirmary, Oxford, U.K.
Leslie
R A
RA
Grahame-Smith
D G
DG
Harvey
J M
JM
D000368:Aged; D001345:Autoradiography; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008526:Medulla Oblongata / Q000187:drug effects / Q000378:metabolism; D011985:Receptors, Serotonin / Q000378:metabolism*; D012702:Serotonin Antagonists / Q000494:pharmacology*; D014630:Vagus Nerve / Q000378:metabolism*
D011985:Receptors, Serotonin; D012702:Serotonin Antagonists