title: [Analysis of proteins in normal and regenerating rat liver using two-dimensional electrophoresis].
abstract: The major proteins of homogenate, cytosol, nuclei and nuclear membrane extract from normal and regenerating rat liver were studied by two-dimensional electrophoresis with a view of detecting proteins involved in DNA replication regulation. Essential quantitative differences in three out of 200 polypeptides separated as spots and dyed with Coomassie R-250 on two-dimensional maps were revealed. The content of the p38 nuclear protein (Mr congruent to 38 kD, pI congruent to 4) increases 6-8-fold in the S-phase. The level of another nuclear protein, p50 (Mr congruent to 50 kD, pI congruent to 6.5) decreases 2-3-fold. The cytoplasmic protein p35 (Mr congruent to 35 kD, pI congruent to 8) also decreases 2-3-fold. Moreover, the p40 protein (Mr congruent to 40 kD, pI congruent to 6) whose content in the nuclei sharply rises up to 20 times after sham operation was revealed.
authors.forename: S N
authors.initials: SN
authors.lastname: Naryzhnyĭ
authors.forename: V M
authors.initials: VM
authors.lastname: Krutiakov
mesh_terms: D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D008099:Liver / Q000032:analysis*; D008115:Liver Regeneration; D008297:Male; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis*; D051381:Rats
chemical_list: D011506:Proteins
vernacular_title: Analiz belkov normal'noĭ i regeneriruiushcheĭ pecheni krysy metodom dvumernogo élektroforeza.