title: Investigation of a persistently raised serum AST.
abstract: The AST responsible for a raised enzyme activity, with otherwise normal biochemistry, in a single individual over a 16-month period has been characterized as an IgG (lambda)-AST complex by (i) estimation of the molecular size by gel exclusion chromatography on Sephadex G200, (ii) agarose gel electrophoresis, (iii) immunoprecipitation by anti-immunoglobulin antisera and (iv) adsorption on to protein A-agarose.
authors.affiliation: Department of Chemical Pathology, Norfolk and Norwich Hospital, UK.
authors.forename: C M
authors.initials: CM
authors.lastname: Dawson
authors.forename: M S
authors.initials: MS
authors.lastname: Connelly
authors.forename: H J
authors.initials: HJ
authors.lastname: Kennedy
authors.forename: G D
authors.initials: GD
authors.lastname: Howe
authors.forename: T R
authors.initials: TR
authors.lastname: Tickner
mesh_terms: D000327:Adsorption; D000328:Adult; D001219:Aspartate Aminotransferases / Q000097:blood* / Q000276:immunology; D001797:Blood Protein Electrophoresis; D002850:Chromatography, Gel; D003600:Cytosol / Q000201:enzymology; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007527:Isoenzymes / Q000097:blood; D008928:Mitochondria / Q000201:enzymology; D013205:Staphylococcal Protein A
chemical_list: D007074:Immunoglobulin G; D007527:Isoenzymes; D013205:Staphylococcal Protein A; D001219:Aspartate Aminotransferases