O dealkylation and aliphatic and aromatic hydroxylation of 3-methoxy-17 beta-estradiol by Aspergillus alliaceus.
Aspergillus alliaceus UI 315 was examined for its ability to metabolize 3-methoxy-17 beta-estradiol. Preparative-scale incubations with this substrate afforded good yields of 6 beta-hydroxy-17 beta-estradiol, 4-hydroxy-17 beta-estradiol, and 4,6 beta-dihydroxy-17 beta-estradiol, which were identified by high-pressure liquid chromatography, 1H and 13C nuclear magnetic resonance, and high-resolution mass spectrometry.
Williamson
J S
JS
Biocatalysis Research Group, College of Pharmacy, University of Iowa, Iowa City 52242.
Van Orden
D E
DE
Rosazza
J P
JP
D001230:Aspergillus / Q000378:metabolism*; D001711:Biotransformation; D002845:Chromatography; D003640:Dealkylation; D004958:Estradiol / Q000031:analogs & derivatives / Q000378:metabolism* / Q000493:pharmacokinetics; D006838:Hydrocarbons / Q000378:metabolism*; D006900:Hydroxylation; D009682:Magnetic Resonance Spectroscopy
D006838:Hydrocarbons; C025004:estradiol-17 beta-3-methyl ether; D004958:Estradiol