Apparent sequence preference in cleavage of linear B-DNA by the Cu(II):thiol system.
Cleavage of a linear tyr T fragment of duplex DNA by Cu(II):thiol combinations occurs with apparent sequence preference. The sequence selectivity was unaffected by the size, chirality or substitution pattern of the monothiol partner, presumably indicating that the Cu(II):RSH system is probing some local conformational/dynamic aspect of the DNA.
John
D C
DC
Department of Pharmacy, University of Manchester, UK.
Douglas
K T
KT
D015097:3-Mercaptopropionic Acid / Q000494:pharmacology; D002413:Cations, Divalent; D003300:Copper / Q000494:pharmacology*; D003545:Cysteine / Q000494:pharmacology; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D004278:DNA, Superhelical / Q000187:drug effects; D015246:Deoxyribonuclease EcoRI; D004229:Dithiothreitol / Q000494:pharmacology; D005978:Glutathione / Q000494:pharmacology; D009690:Nucleic Acid Conformation; D010957:Plasmids; D012996:Solutions; D013438:Sulfhydryl Compounds / Q000494:pharmacology*; D014443:Tyrosine
D002413:Cations, Divalent; D004278:DNA, Superhelical; D012996:Solutions; D013438:Sulfhydryl Compounds; D014443:Tyrosine; D003300:Copper; D004247:DNA; D015097:3-Mercaptopropionic Acid; D015246:Deoxyribonuclease EcoRI; D005978:Glutathione; D003545:Cysteine; D004229:Dithiothreitol