title: Effect of dexamethasone on arachidonate metabolism in isolated mouse thymocytes.
abstract: In order to investigate the effects of glucocorticoids on arachidonate release and metabolism in mouse thymocytes, we have studied both the action of dexamethasone on arachidonate release from pre-labeled cells and its effect on tracer uptake and metabolism. Our results indicate, first, that dexamethasone failed in this experimental model to affect phospholipase activity; second, that glucocorticoids are able to inhibit the transformation of the precursor into prostaglandins and to block simultaneously its acylation into phospholipids; finally that isolated thymocytes secrete significant amounts of 12-HETE, and that this secretion is unaffected by steroid treatment.
authors.affiliation: Inserm U7, Hôpital Necker, Paris, France.
authors.forename: D
authors.initials: D
authors.lastname: Duval
mesh_terms: D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism*; D002845:Chromatography; D003907:Dexamethasone / Q000494:pharmacology*; D005260:Female; D066298:In Vitro Techniques; D007700:Kinetics; D051379:Mice; D008810:Mice, Inbred C57BL; D011453:Prostaglandins / Q000097:blood; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*
chemical_list: D001095:Arachidonic Acids; D011453:Prostaglandins; D003907:Dexamethasone