18-substituted steroids--Part 17. 2 alpha-hydroxylated liver metabolites of aldosterone identified by high-field [1H]NMR spectroscopy.
11 beta,18-Epoxy-2 alpha,3 alpha,18,21-tetrahydroxy-5 alpha,17 alpha- pregnan-20-one (2 alpha-hydroxy-3 alpha,5 alpha-tetrahydro-17-isoaldosterone) and its apo isomer have been identified by high-field NMR studies, supported by thermospray HPLC/MS, to be among the major polar metabolites formed from incubation of aldosterone with rat liver microsomal fraction. Indications that unreduced 2 alpha-hydroxy-aldosterone is also present among the metabolites have still to be confirmed.
Latif
S A
SA
Department of Laboratory Medicine, Miriam Hospital, Providence, Rhode Island.
Morris
D J
DJ
Wei
L
L
Kirk
D N
DN
Burke
P J
PJ
Toms
H C
HC
Shackleton
C H
CH
D000450:Aldosterone / Q000378:metabolism*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D013058:Mass Spectrometry; D008862:Microsomes, Liver / Q000378:metabolism*; D011278:Pregnanes / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D011278:Pregnanes; C062591:11,18-epoxy-2,3,18,21-tetrahydroxypregnan-20-one; D000450:Aldosterone