[The action of alpha-bungarotoxin on the acetylcholine responses of the membrane of snail neurons].
The effect of alpha-bungarotoxin (alpha-BT) on two types of acetylcholine (ACh)-induced currents distinguished by their desensitization properties was studied on the isolated dialyzed Helix neurons by a concentration clamp method. It was shown that alpha-BT depressed both types of ACh responses and this action was reversible in the majority of experiments. The depressive action of alpha-BT on ACh-induced currents occurred only in the presence of albumin, though albumin itself had no effect on the ACh responses. The concentration dependence of the blocking effect of alpha-BT on ACh-induced currents in both types of neurons was studied. The calculated values of alpha-BT concentration which caused 50% depression of the ACh-induced current were (13.85 +/- 1.25) X 10(-8) and (5.56 +/- 1.0) X 10(-8) g/ml for A and B types of neurons, respectively.
Arvanov
V L
VL
Stepanian
A S
AS
Azatian
K V
KV
Aĭrapetian
S N
SN
D000109:Acetylcholine / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D000818:Animals; D002038:Bungarotoxins / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D006372:Helix, Snails; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000187:drug effects* / Q000502:physiology
D002038:Bungarotoxins; D000109:Acetylcholine
Deĭstvie al'fa-bungarotoksina na atsetilkholinovye otvety membrany neĭronov ulitki.