The effect of sialoadenectomy on gastric mucosal integrity in the rat: roles of epidermal growth factor and prostaglandin E2.
Removal of the salivary glands (SALX) in rats has been shown to increase the susceptibility of gastric mucosa to ulcerogens. In the present study, we have investigated the role of specific salivary glands in this response. In addition, we have examined whether a functional link exists between the salivary glands, epidermal growth factor (EGF), and prostaglandin E2 (PGE2) by determining whether SALX decreases the responsiveness of the mucosa to the protective actions of either of both of these agents. Removal of the parotid salivary glands did not significantly increase ulceration in response to intragastric administration of 100% w/v ethanol. Animals were examined 60 min after ethanol administration. Removal of the submandibular-sublingual gland complexes was associated with a significant increase in the area of mucosal damage and a decrease in gastric pit depth in ethanol-treated animals when compared with sham-operated control rats. Furthermore, in both SALX and control animals, exogenous PGE2 and EGF resulted in a dose-dependent reduction in both groups of animals, although the protective effects of PGE2 and EGF were attenuated in SALX rats. PGE2 and EGF administered in combination resulted in the same degree of protection in both SALX and control rats. Sialoadenectomy resulted in a reduction in mucosal PGE2 synthesis. EGF administration did not consistently increase mucosal PGE2 synthesis. Conversely, sialoadenectomy did not reduce mucosal levels of EGF nor did exogenous PGE2 consistently increase salivary or mucosal content of EGF.(ABSTRACT TRUNCATED AT 250 WORDS)
Tepperman
B L
BL
Department of Physiology, Faculty of Medicine, University of Western Ontario, London, Canada.
Kiernan
J A
JA
Soper
B D
BD
D000818:Animals; D015232:Dinoprostone / Q000096:biosynthesis / Q000276:immunology / Q000502:physiology*; D004815:Epidermal Growth Factor / Q000502:physiology*; D000431:Ethanol / Q000494:pharmacology; D005753:Gastric Mucosa / Q000502:physiology*; D008297:Male; D009093:Mucus / Q000502:physiology; D010306:Parotid Gland / Q000502:physiology; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D012469:Salivary Glands / Q000502:physiology*; D013363:Submandibular Gland / Q000502:physiology
D000431:Ethanol; D004815:Epidermal Growth Factor; D015232:Dinoprostone