Capillary gas-chromatographic analysis of monosaccharides: improvements and comparisons using trifluoroacetylation and trimethylsilylation of sugar O-benzyl- and O-methyl-oximes.
Two new procedures for the gas-chromatographic analysis of monosaccharides are reported. One involves derivatization of the sugars by reaction with O-benzylhydroxylamine followed by trifluoroacetylation with N-methylbis(trifluoroacetamide) and chromatography on a DB-1701 capillary column. This technique probably provides the best resolution achieved to date of the C3-C6 aldoses, as well as of the corresponding alditols. Ketoses can be qualitatively analyzed by this method, but complications interfere with their quantitative analysis. The second procedure also involves initial derivatization as the O-benzyloxime, but is followed by trimethylsilylation with 1-trimethylsilylimidazole, and chromatography on a DB-17 column. This technique is particularly useful for C5 sugars, C6 ketoses, and mixtures of sugars, alditols, and/or lactones. A number of additional, critical, observations on the derivatization and capillary gas-chromatographic analysis of monosaccharides are described.
Andrews
M A
MA
Chemistry Department, Brookhaven National Laboratory, Upton, New York 11973.
D000081:Acetamides; D000107:Acetylation; D002849:Chromatography, Gas / Q000379:methods; D005463:Fluoroacetates; D006898:Hydroxylamines; D007093:Imidazoles; D007202:Indicators and Reagents; D008745:Methylation; D009005:Monosaccharides / Q000032:analysis*; D014297:Trimethylsilyl Compounds
D000081:Acetamides; D005463:Fluoroacetates; D006898:Hydroxylamines; D007093:Imidazoles; D007202:Indicators and Reagents; D009005:Monosaccharides; D014297:Trimethylsilyl Compounds; C054025:1-(trimethylsilyl)-1H-imidazole; C017064:benzyloxyamine; C014666:N-methyl-bis(trifluoroacetamide)