Mass spectrometric identification and quantification of 5-methoxytryptophol in quail retina.
The occurrence of 5-methoxytryptophol (5-MTL) in the quail retina was investigated by capillary column gas chromatography/mass spectrometry/selected ion monitoring using a deuterated internal standard. Based on ion intensity ratios in the mass spectra of pentafluoropropionyl and heptafluorobutyryl derivatives of 5-MTL and deuterated 5-MTL, 5-MTL was unequivocally identified in the quail retina. Similar to the circadian rhythm of retinal melatonin, retinal 5-MTL also exhibited a diurnal variation with high levels at mid-dark. However, no significant correlation between the diurnal levels of 5-MTL and melatonin was observed in the quail retina at mid-light or mid-dark.
Tsang
C W
CW
Department of Applied Biology & Chemical Technology, Hong Kong Polytechnic.
Chan
S F
SF
Lee
P P
PP
Pang
S F
SF
D000818:Animals; D002940:Circadian Rhythm; D003903:Deuterium; D005466:Fluorocarbons; D008401:Gas Chromatography-Mass Spectrometry; D007211:Indoles / Q000032:analysis*; D013058:Mass Spectrometry; D008550:Melatonin / Q000032:analysis; D011784:Quail / Q000378:metabolism*; D012160:Retina / Q000032:analysis*
D005466:Fluorocarbons; D007211:Indoles; C033094:perfluorobutyric acid; C033093:perfluoropropionic acid; C002465:methoxytryptophol; D003903:Deuterium; D008550:Melatonin