17 beta-Hydroxysteroid oxidoreductase activity in the endometrium of normal women and patients with pelvic pain and polycystic ovaries.
17 beta-Hydroxysteroid oxidoreductase (17-OHSD) activity in the endometrium of women with pelvic pain syndrome (PPS) and/or polycystic ovaries (PCO) was compared with that of a control group. In both groups there was a 10-fold increase in 17-OHSD activity in secretory phase tissue compared with that of the proliferative phase, measured by both oxidative and reduction pathways, and a highly significant correlation between the two directions (P less than 0.001). In normal subjects, the ratio of activity measured under oxidative conditions: reducing conditions, at all stages of the cycle except late proliferative phase, was 2.1-2.9. In the late proliferative phase the ratio was 5.5 which was significantly different from other stages of the cycle. Similar ratios were found for the PPS/PCO group (proliferative phase 2.5, secretory phase 5.6); these were also significantly different (P less than 0.01). On the basis of this study oestrogen metabolism in the endometrium of women with PPS and/or PCO appears to be no different from that of normal subjects. Measurement of enzyme activity in high speed soluble and particulate fractions of endometrial homogenate indicated the presence of two activities with different cofactor requirements. Gel filtration chromatography of the soluble fraction revealed a single peak of activity coincident with a molecular weight of 30 kDa with a strong preference for NAD + as cofactor. These preliminary findings suggest the presence of both soluble and particulate forms of 17-OHSD activity in the endometrium.
Bonney
R C
RC
Department of Chemical Pathology, St. Mary's Hospital Medical School, London, U.K.
Dalby
M C
MC
Newton
C J
CJ
Franks
S
S
Beard
R W
RW
James
V H
VH
D015415:Biomarkers / Q000032:analysis*; D004717:Endometrium / Q000201:enzymology*; D004958:Estradiol / Q000378:metabolism; D004970:Estrone / Q000096:biosynthesis; D005260:Female; D006801:Humans; D006913:Hydroxysteroid Dehydrogenases / Q000032:analysis / Q000378:metabolism*; D000292:Pelvic Inflammatory Disease / Q000201:enzymology*; D011085:Polycystic Ovary Syndrome / Q000201:enzymology*; D012016:Reference Values; D013347:Subcellular Fractions / Q000201:enzymology
D015415:Biomarkers; D004970:Estrone; D004958:Estradiol; D006913:Hydroxysteroid Dehydrogenases; C031932:7 beta-hydroxysteroid dehydrogenase