Effect of butyrate on the expression of the human preprourokinase gene introduced into Chinese hamster ovary cells.
A plasmid containing the human preprourokinase gene cDNA under the control of the simian virus 40 early region promoter was introduced into CHO-K1 cells and recombinant cell lines secreting a relatively high level of urokinase were obtained. In the course of studying the effects of various agents on the recombinant cell lines, we found that exposure of recombinant cells to 5 mM butyrate for 24 hours resulted in a 2-3 fold increase in urokinase production. The induction by butyrate was dose-dependent. The half maximal dose was approximately 2 mM; maximal stimulation occurred at 5-10 mM. Cell growth, on the other hand, was inhibited by butyrate concentrations greater than 2.5 mM. The response of cells to butyrate was rapid: a significant increase in urokinase production was observed 6 hours after exposure to 5 mM butyrate. Butyrate treatment increased not only the extracellular level but also the intracellular level of urokinase.
Okabayashi
K
K
Biotechnology Center, Green Cross Corporation, Osaka, Japan.
Kanéda
T
T
Arimura
H
H
D000818:Animals; D002087:Butyrates / Q000494:pharmacology*; D002460:Cell Line; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D004792:Enzyme Precursors / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D005796:Genes; D006801:Humans; D010957:Plasmids; D011498:Protein Precursors / Q000235:genetics*; D015183:Restriction Mapping; D013997:Time Factors; D014162:Transfection; D014568:Urokinase-Type Plasminogen Activator / Q000096:biosynthesis / Q000235:genetics*
D002087:Butyrates; D004792:Enzyme Precursors; D011498:Protein Precursors; C049163:preprourokinase; D014568:Urokinase-Type Plasminogen Activator