Calculation of membrane potential in synaptosomes with use of a lipophilic cation (tetraphenylphosphonium).
To estimate membrane potential in synaptosomes with the use of tetraphenylphosphonium (TPP+), an equation relating the amount of TPP+ accumulated in synaptosomes with membrane potential was derived from the following two assumptions. (1) TPP+ molecules were distributed into plasma membranes, mitochondria and cytosol of synaptosomes. (2) TPP+ achieves a Nernst equilibrium across both the synaptosomal and inner mitochondrial membranes. We propose three methods for calculation of membrane potential using this equation. The concentration of TPP+ was measured under various controlled conditions with an electrode selective for TPP+. The amount of TPP+ accumulated in synaptosomes was determined by measuring the difference between its initial concentration and the concentration after addition of synaptosomes, and membrane potential was estimated by the three methods. The resting potential of synaptosomes was estimated to be -75 to -90 mV by all of these methods. Membrane potentials under various controlled conditions were calculated, and the characteristics of the methods for estimation of membrane potential and those of membrane potential obtained by the methods are discussed.
Aiuchi
T
T
Matsunaga
M
M
Nakaya
K
K
Nakamura
Y
Y
D000818:Animals; D004734:Energy Metabolism; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D009861:Onium Compounds; D009943:Organophosphorus Compounds; D051381:Rats; D013574:Synaptosomes / Q000187:drug effects*
D007202:Indicators and Reagents; D009861:Onium Compounds; D009943:Organophosphorus Compounds; C013289:tetraphenylphosphonium