title: Effect of immunoneutralisation of cholecystokinin on bombesin-stimulated pancreatic enzyme secretion in the rat.
abstract: Infusion of bombesin stimulates plasma cholecystokinin (CCK) and pancreatic enzyme secretion in various species, including the rat. This study was undertaken in two groups of four conscious rats with a cannulated pancreatic duct to determine the role of endogenously released CCK in mediating the effect of bombesin on pancreatic enzyme secretion. Infusion of 2 ml CCK antiserum or normal rabbit serum for 40 min was followed 10 min later by infusion of 18 pmol/kg bombesin for 30 min and after an interval of 90 min by infusion of 24 pmol/kg CCK for 30 min. After administration of control rabbit serum, pancreatic protein secretion increased by 3.2 +/- 1.0 mg/30 min during bombesin and 4.0 +/- 1.5 mg/30 min during CCK, while the plasma CCK increments were 1.7 +/- 0.5 pM and 7.0 +/- 0.9 pM for the bombesin and CCK infusions, respectively. Immunoneutralisation with the CCK antiserum did not significantly affect bombesin-stimulated pancreatic protein secretion (3.6 +/- 1.3 mg/30 min), but almost abolished the pancreatic protein response to CCK (0.5 +/- 0.2 mg/30 min). It is therefore concluded that CCK is not an important mediator of the stimulatory effect of bombesin on the pancreas in the rat.
authors.affiliation: Department of Gastroenterology-Hepatology, University Hospital, Leiden, The Netherlands.
authors.forename: C B
authors.initials: CB
authors.lastname: Lamers
authors.forename: A J
authors.initials: AJ
authors.lastname: de Jong
authors.forename: J C
authors.initials: JC
authors.lastname: Hafkenscheid
authors.forename: J B
authors.initials: JB
authors.lastname: Jansen
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D001839:Bombesin / Q000494:pharmacology*; D002766:Cholecystokinin / Q000097:blood* / Q000276:immunology; D008297:Male; D010179:Pancreas / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000906:Antibodies; D002766:Cholecystokinin; D001839:Bombesin