Effect of pyridostigmine and syntostigmine pretreatment on the inhibition of acetylcholinesterases by o-pinacolyl-methylphosphonofluoridate. In vitro experiments with rat tissues.
The inhibition kinetics of O-pinacolyl-methylphosphonofluoridate and two carbamates (pyridostigmine and syntostigmine) on the acetylcholinesterase from rat erythrocytes, brain and diaphragm have been studied in vitro. The affinity of O-pinacolyl-methylphosphonofluoridate to carbamates pretreated acetylcholinesterase was tested. In vitro addition of pyridostigmine and/or syntostigmine to acetylcholinesterase prior to the organophosphate provided a weak protection against acetylcholinesterase inhibition by this potent organo-phosphorous inhibitor.
Patocka
J
J
Purkynĕ Medical Research Institute, Hradec Králové, Czechoslovakia.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D003964:Diaphragm / Q000187:drug effects / Q000201:enzymology; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology*; D005260:Female; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D009388:Neostigmine / Q000494:pharmacology*; D011729:Pyridostigmine Bromide / Q000494:pharmacology*; D051381:Rats; D012132:Respiratory Muscles / Q000187:drug effects / Q000201:enzymology*; D012999:Soman / Q000494:pharmacology*
D002800:Cholinesterase Inhibitors; D009388:Neostigmine; D012999:Soman; D011729:Pyridostigmine Bromide