An indirect asymmetrical sandwich ELISA using anti-allotype antibodies for the specific and quantitative measurement of mouse IgG2a of Igh-1b allotype.
We recently described an indirect double sandwich ELISA (Klein-Schneegans et al., J. Immunol. Methods (1989) 119, 117) which permits the specific and quantitative measurement of mouse IgM, IgA and IgG subclasses with one major exception: IgG2a of the b allotype (Igh-1b in mouse strains such as C57BL/6) could not be reliably quantitated even by a very specific and sensitive asymmetrical sandwich ELISA (using two different anti-IgG2a isotype antibodies for capture and for detection). We now describe a similar method based on the use of two different anti-IgG2a allotype antibodies for the capture and detection of IgG2a in the serum of Igh-1b mouse strains.
Klein-Schneegans
A S
AS
Laboratory of Immunology, Louis Pasteur University Strasbourg, Illkirch, France.
Kuntz
L
L
Fonteneau
P
P
Loor
F
F
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D007126:Immunoglobulin Allotypes / Q000032:analysis* / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis*; D007132:Immunoglobulin Isotypes / Q000032:analysis; D051379:Mice; D008815:Mice, Inbred Strains / Q000276:immunology; D008817:Mice, Mutant Strains / Q000276:immunology
D007126:Immunoglobulin Allotypes; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes