title: Presence of glucagon-(1-21)-Like immunoreactive substance in the dog small intestinal mucosa.
abstract: By using an antiserum (K291) specifically directed to the C-terminal of glucagon-(1-21)-peptide, we demonstrated the presence of glucagon-(1-21)-like immunoreactivity (G21-IR) in the dog intestine. G21-IR was found to be widely distributed throughout the small intestine and colon in parallel with the distribution of glucagon-like immunoreactivity (GLI), measured by N-terminal glucagon antiserum (OAL196). The subsequent analyses by gel filtration and three HPLC columns (reverse phase, ion exchange and further reverse phase columns) showed that G21-IR consisted of three main peaks, and the smallest molecular form of G21-IR is identical to glucagon-(1-21)-peptide.
authors.affiliation: Second Department of Internal Medicine, Osaka University Medical School, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Itoh
authors.forename: M
authors.initials: M
authors.lastname: Namba
authors.forename: N
authors.initials: N
authors.lastname: Watanabe
authors.forename: T
authors.initials: T
authors.lastname: Ohara
authors.forename: T
authors.initials: T
authors.lastname: Matsuyama
authors.forename: R
authors.initials: R
authors.lastname: Komatsu
authors.forename: Y
authors.initials: Y
authors.lastname: Shinomura
authors.forename: N
authors.initials: N
authors.lastname: Kono
authors.forename: S
authors.initials: S
authors.lastname: Tarui
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D005934:Glucagon / Q000032:analysis*; D004763:Glucagon-Like Peptides; D007413:Intestinal Mucosa / Q000032:analysis*; D007421:Intestine, Small / Q000032:analysis*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis*; D010455:Peptides / Q000032:analysis; D011863:Radioimmunoassay; D014018:Tissue Distribution
chemical_list: D010446:Peptide Fragments; D010455:Peptides; C043386:glucagon (1-21); D004763:Glucagon-Like Peptides; C015236:glucagon-like-immunoreactivity; D005934:Glucagon