Antigenic characteristics of circulating granulocyte-macrophage progenitors (CFU-GM) in idiopathic myelofibrosis and polycythaemia vera defined by monoclonal antibodies.
The antigenic characteristics of "early" (type 1) and "late" (type 2) granulocyte-macrophage progenitor cells (CFU-GM) from patients with polycythaemia vera (PV) and idiopathic myelofibrosis (MF) were studied using antimyeloid murine monoclonal antibodies (McAbs) and anti-HLA-DR McAb (L243) in a complement dependent cytotoxic assay followed by culture in methyl cellulose. The results were compared with the previously published data on the antigen phenotype of CFU-GM from normal bone marrow and peripheral blood. McAbs L243, MY9, S3-13 and S17-25 reacted with lower percentage of CFU-GM from patients with PV and MF than with their normal counterparts MCAbs R1.B19 and WGHS 29.1, which recognized the antigens on "late" CFU-GM (type 2), reacted with a high proportion of day 7 CFU-GM from MF peripheral blood and normal marrow but did not react with CFU-GM from PV and normal peripheral blood. The antigens recognized by two other McAbs (53/10 and PM81) were present on some of normal CFU-GM and were not found on CFU-GM from MF and PV peripheral blood. Our data suggest that CFU-GM in the circulation in MF differ from those in normal blood, perhaps because they are released from the spleen which in this disease has haemopoietic function.
Robak
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Department of Pharmacology, Medical Academy, Lódź.
D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D003601:Cytotoxicity Tests, Immunologic; D006098:Granulocytes / Q000276:immunology*; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D008264:Macrophages / Q000276:immunology*; D008875:Middle Aged; D011087:Polycythemia Vera / Q000276:immunology*; D055728:Primary Myelofibrosis / Q000276:immunology*; D013234:Stem Cells / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006680:HLA Antigens