Subcellular compartmentation of dolichol taken up by mouse leukemia cells.
[1-3H]Dolichol was intraperitoneally incubated with mouse leukemia Lcb 35 cells. The radioactive lipid taken up by the cells was subsequently recovered in two fractions separated by sucrose density gradient centrifugation: a particular, most probably, lysosomal fraction and a cytoplasmic one. Thin-layer chromatography of radioactive lipids present in both fractions revealed that majority of dolichol was unchanged and only a small part esterified with fatty acids. Distribution of dolichol in various mouse organs was examined. Of all the organs examined, spleen accumulated the highest amount of radioactivity.
Jankowski
W
W
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland.
Szkopińska
A
A
Kula-Swiezewska
E
E
D000818:Animals; D001202:Ascitic Fluid; D002451:Cell Compartmentation; D002499:Centrifugation, Density Gradient; D004286:Dolichols / Q000493:pharmacokinetics*; D007942:Leukemia, Experimental / Q000378:metabolism*; D008055:Lipids / Q000302:isolation & purification; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D014407:Tumor Cells, Cultured
D004286:Dolichols; D008055:Lipids