Further characterization of the impaired protective function in mice fed with lipid diet.
Female mice were maintained on lipid diet for 20 days. The nonspecific and immunological defense capability was determined by in vitro and in vivo methods. It was found that mice held mostly on a lipid diet demonstrate an all-round lowered response. Following 20 days of lipid diet the splenocytes exhibit: (1) an inversed lipid-protein ratio; (2) an inability to respond to sheep erythrocytes; (3) a reduction in [3H] thymidine incorporation in splenocytes stimulated with lipopolysaccharide (LPS) or with concanavalin A; (4) a reduction in the number of cells bearing surface immunoglobulins in splenocytes stimulated with LPS; (5) an inhibition of phagocytosis and intracellular killing in macrophages; (6) a lowering in granulocyte chemotaxis and adherence capacity; (7) a higher mortality to LPS after loading with galactosamine; and (8) a lowered complement activity even following LPS activation.
Galdiero
F
F
Istituto di Microbiologia, I Facoltà di Medicina e Chirurgia, Università di Napoli, Italy.
Romano Carratelli
C
C
Nuzzo
I
I
Bentivoglio
C
C
Galdiero
E
E
D000818:Animals; D000917:Antibody Formation; D002470:Cell Survival; D002634:Chemotaxis, Leukocyte; D015941:Complement Hemolytic Activity Assay; D003208:Concanavalin A / Q000494:pharmacology; D004036:Diet, Atherogenic; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D005688:Galactosamine / Q000378:metabolism; D007109:Immunity; D066298:In Vitro Techniques; D007957:Leukocyte Adherence Inhibition Test; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D010587:Phagocytosis; D013154:Spleen / Q000276:immunology
D008070:Lipopolysaccharides; D003208:Concanavalin A; D005688:Galactosamine