[Detection of Erysipelothrix rhusiopathiae specific antibodies in the serum of experimentally-infected swine by ELISA and immunoblotting].
In an attempt to characterize the immuno response of the animal organism to experimental infection, ELISA and immunoblotting were used to test the antibody levels of erysipelas hyperimmune sera (HIS) which had been induced by Erysipelothrix rhusiopathiae. Also, 5 pigs were accidentally chosen to check on individual curves of antibody formation, following periodical inoculation up to HIS collection. All HIS verified and confirmed by the mouse protective test responded by high titres. Yet, the results so far obtained from HIS titration have failed to be conclusive as to valency. The animals selected for these experiments exhibited differentiated antibody levels during the phase of immunisation, although they were at one and the same level, when measured by the final titre, following last boostering. The desired rise in antibody levels was achieved only by the 4th to 9th boostering in almost all cases. Extinction values determined by ELISA were confirmed or supplemented by the results obtained from immunoblotting.
Dahms
H
H
Schilow
W F
WF
Hagemann
G
G
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D004888:Erysipelothrix / Q000276:immunology*; D004889:Erysipelothrix Infections / Q000276:immunology*; D015151:Immunoblotting; D013552:Swine; D013554:Swine Erysipelas / Q000276:immunology*
D000907:Antibodies, Bacterial
Nachweis von Erysipelothrix-rhusiopathiae-spezifischen Antikörpern in Seren experimentell infizierter Schweine mit ELISA und Immunoblotting.