title: The purple to blue transition of bacteriorhodopsin is accompanied by a loss of the hexagonal lattice and a conformational change.
abstract: X-ray diffraction measurements show that in contrast to the purple membrane, the bacteriorhodopsin molecules are not organized in a hexagonal lattice in the deionized blue membrane. Addition of Ca2+ restores both the purple color and the normal (63 A) hexagonal protein lattice. In the blue state, the circular dichroism spectrum in the visible has the typical exciton features indicating that a trimeric structure is retained. Time-resolved linear dichroism measurements show that the blue patch rotates in aqueous suspension with a mean correlation time of 11 ms and provide no evidence for rotational mobility of bacteriorhodopsin within the membrane. The circular dichroism spectra of the blue and the Ca2+-regenerated purple state in the far-UV are different, indicating a small change in secondary structure. The thermal stability of the blue membrane is much smaller than that of the purple membrane. At pH 5.0, the irreversible denaturation transition of the blue form has a midpoint at 61 degrees C. The photocycle of the blue membrane (lambda ex 590 nm) has an L intermediate around 540 nm whose decay is slowed down into the millisecond time range (5 ms). Light-dark adaptation in the blue membrane is rapid with an exponential decay time of 38 s at 25 degrees C. The purple to blue transition apparently involves a conformational change in the protein leading to a change in the aggregation state from a highly ordered and stable hexagonal lattice to a disordered array of thermally more labile trimers.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Physics, Freie Universität Berlin, FRG.
authors.forename: M P
authors.initials: MP
authors.lastname: Heyn
authors.forename: C
authors.initials: C
authors.lastname: Dudda
authors.forename: H
authors.initials: H
authors.lastname: Otto
authors.forename: F
authors.initials: F
authors.lastname: Seiff
authors.forename: I
authors.initials: I
authors.lastname: Wallat
mesh_terms: D001436:Bacteriorhodopsins; D002462:Cell Membrane / Q000502:physiology; D002942:Circular Dichroism; D003116:Color; D011487:Protein Conformation; D014961:X-Ray Diffraction
chemical_list: D001436:Bacteriorhodopsins