Anti-idiotype response to selected neutralizing monoclonal antibodies generated against adenovirus type 2 fiber.
A series of monoclonal antibodies was prepared against purified adenovirus type 2 fiber. These antibodies were produced by both classical techniques and by electrofusion. Fifty antibodies were obtained, and five were selected which had the following characteristics: (i) they were able to reduce adenovirus 2 infection of Hep-2 cells; (ii) they had different light-chain structures and (iii) they did not react with denatured fiber. The five antibodies (AdF18, AdF38, AdF41, AdF2D3, AdF3B1) recognized between them three periodate-insensitive epitopes on the fiber. AdF18 bound to both adenovirus type 2 and type 5; all the others were specific to type 2. Polyclonal anti-idiotype antibodies to these antibodies were produced in rabbits and were purified extensively on immunoaffinity columns. Anti-idiotypes to AdF38 and AdF41 were able to inhibit virus infectivity, and anti-AdF41 completed with fiber for binding to the surface of Hep-2 cells.
Talbot
B G
BG
Département de Biologie, Faculté des Sciences, Université de Sherbrooke, Québ., Canada.
Hallée
J
J
D000256:Adenoviridae / Q000276:immunology*; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D002213:Capsid / Q000276:immunology*; D036022:Capsid Proteins
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D036022:Capsid Proteins; C018315:hexon capsid protein, Adenovirus