Influence of chronic energy intake restriction on intestinal alkaline phosphatase in C3H/Bi mice and autoimmune-prone MRL/lpr,lpr mice.
The influence of chronic energy intake restriction (CEIR) on the level and activity of intestinal alkaline phosphatase was investigated in mice of the autoimmune-prone MRL/lpr,lpr strain and in mice of the autoimmune-resistant C3H/Bi strain. In both strains of mice, CEIR of 40% resulted in a significant increase in intestinal alkaline phosphatase (IAP) specific activity in MRL/lpr,lpr mice after 10 wk of feeding, and in C3H/Bi mice after 6 wk of feeding. An increase in the amount of immunoreactive alkaline phosphatase antigen was also found to be associated with the increased enzyme activity in CEIR mice. These results suggest that a specific induction of an intestinal enzyme occurs or, alternatively, that there is a specific relative decrease in synthesis of intestinal proteins other than IAP as a function of CEIR. Thus, CEIR appears to regulate the expression of proteins in the small intestine in a specific manner.
Dao
M L
ML
University of South Florida, All Children's Hospital, St. Petersburg 33701.
Shao
R
R
Risley
J
J
Good
R A
RA
D000469:Alkaline Phosphatase / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D000941:Antigens / Q000032:analysis; D001327:Autoimmune Diseases / Q000235:genetics / Q000378:metabolism* / Q000401:mortality; D001835:Body Weight; D004591:Electrophoresis, Polyacrylamide Gel; D002149:Energy Intake; D005260:Female; D007421:Intestine, Small / Q000201:enzymology*; D051379:Mice; D008809:Mice, Inbred C3H; D008815:Mice, Inbred Strains; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics
D000941:Antigens; D011506:Proteins; D000469:Alkaline Phosphatase