[Cytofluorometric analysis of thymic lymphocytic subpopulations in mice with B-16 melanoma].
Two colour flow cytometric analysis of the Lyt2 and L3T4 T-cell surface antigens on thymocytes from B16 melanoma bearing mice, reveal an altered cell phenotype. At the early stage of the neoplasia and during the growth, the phenotypes Lyt2+/L3T4- (T suppressor) and Lyt2+/L3T4+ (T immature) decrease. Conversely, Lyt2-/L3T4+ (T helper) increase. These findings suggest the hypothesis that impairment of the immune functions in B16 melanoma bearing mice could be due to a primary thymic alteration.
Savarese
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A
Stirparo
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G
D000818:Animals; D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D000951:Antigens, Neoplasm / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D005434:Flow Cytometry; D007958:Leukocyte Count; D008297:Male; D008546:Melanoma, Experimental / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010641:Phenotype; D013601:T-Lymphocytes / Q000473:pathology*
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000954:Antigens, Surface
Analisi citofluorimetrica delle sottopopolazioni linfocitarie timiche in topi portatori di melanoma B-16.