title: Potassium secretion in response to taurodeoxycholic acid in the newborn rabbit colon.
abstract: The newborn colon fails to secrete Cl in response to concentrations of dihydroxy bile acid that cause Cl secretion in adult colonic tissue in vitro. Bile acids also cause secretion of potassium in adult tissues, but there is no information concerning bile acid effects on potassium transport in newborn colon. We mounted newborn rabbit distal colon in Ussing chambers specially designed for newborn colon and measured potassium transport. Basal potassium transport was secretory. Taurodeoxycholic acid, 100 microM, (TDC) decreased JKnet from -0.76 +/- 0.07 to -0.94 +/- 0.11 microEq cm-2 h-1, p less than 0.05, without increasing Isc. Serosal ouabain, 0.1 mM, abolished the secretory response to TDC. Mucosal Ba2+, a potassium channel blocker in many epithelia, did not inhibit K secretion. Similar serosal exposure to TDC in adult colon tissues decreased JKnet from -0.09 +/- 0.29 to -1.63 microEq cm-2 h-1, p less than 0.01, and increased Isc. We conclude that, although the chloride secretory response to dihydroxy bile acids is absent in the newborn, K secretion is elicited in the newborn, similar to the adult colon.
authors.affiliation: Department of Medicine, University of Texas Medical School, Houston 77225.
authors.forename: G D
authors.initials: GD
authors.lastname: Potter
authors.forename: J H
authors.initials: JH
authors.lastname: Sellin
authors.forename: S M
authors.initials: SM
authors.lastname: Burlingame
authors.forename: R C
authors.initials: RC
authors.lastname: DeSoignie
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001464:Barium / Q000494:pharmacology; D002712:Chlorides / Q000378:metabolism; D003106:Colon / Q000187:drug effects* / Q000378:metabolism; D003840:Deoxycholic Acid / Q000031:analogs & derivatives*; D005260:Female; D007413:Intestinal Mucosa / Q000187:drug effects; D008297:Male; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism*; D011817:Rabbits; D013657:Taurodeoxycholic Acid / Q000494:pharmacology*
chemical_list: D002712:Chlorides; D003840:Deoxycholic Acid; D001464:Barium; D013657:Taurodeoxycholic Acid; D010042:Ouabain; D011188:Potassium