O-specific polysaccharides of Hafnia alvei lipopolysaccharides isolated from two serologically related strains: ATCC 13337 and 1187. A serological and structural study using chemical methods, gas chromatography/mass spectrometry and NMR spectroscopy at 500 MHz.
The O-specific polysaccharides of Hafnia alvei ATCC 13337 standard strain and 1187 strain have been isolated and characterized. By means of 1H-NMR spectroscopy, methylation analysis and periodate oxidation, the repeating unit of the polysaccharides could be allocated the respective structures. (formula; see text) where Acyl = D-3-hydroxybutyryl, and 3-O-acetylation was to about 66%. The structural similarity of the polysaccharides was confirmed in the serological study; their epitopes were determined and the importance of various structural elements for the serological specificity was discussed.
Gamian
A
A
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Wroclaw, Poland.
Romanowska
E
E
Opferkuch
H J
HJ
Hauck
M
M
Dabrowski
J
J
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004755:Enterobacteriaceae / Q000276:immunology*; D008401:Gas Chromatography-Mass Spectrometry; D008070:Lipopolysaccharides; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000302:isolation & purification*
D008070:Lipopolysaccharides; D011134:Polysaccharides