[Determination of fetal sex by amplification of Y-chromosome-specific DNA].
This paper describes a rapid and highly sensitive method for determination of fetal sex. Y-chromosome-specific sequences as well as Alu-specific sequences were amplified with polymerase chain reaction (PCR). Then fetal sex was determined by comparison of the two amplified DNA sequences. Polymerase chain reaction can be performed on lysed amniotic fluid cells or chorionic villus samples or dried blood spots on filter paper blot without prior DNA extraction. The analysis of the amplified DNA was performed immediately by agarose gel electrophoresis without DNA hybridization with radioactive probe. By using this method, determination of sex was performed on 3 fetuses at risk for DMD, and on 2 fetuses at risk for hemophilia, prenatal detection was confirmed by examination of the neonates.
Huang
S Z
SZ
D004247:DNA / Q000235:genetics; D005260:Female; D005784:Gene Amplification; D006467:Hemophilia A / Q000175:diagnosis*; D006801:Humans; D009136:Muscular Dystrophies / Q000175:diagnosis*; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011296:Prenatal Diagnosis; D012732:Sex Determination Analysis; D014998:Y Chromosome
D004247:DNA