The molecular structure of Okazaki fragment r(CGCA)d(AAAAAGCG):d(CGCTTTTTTGCG) in solution.
The hetero duplex molecule, r(CGCA)d(AAAAAGCG):d(CGCTTTTTTGCG) which corresponds to Okazaki fragment was synthesized and its molecular structure has been analyzed by NMR study. The RNA strand of RNA-DNA hybrid region adopts A-form and DNA strand of the same region deviates from the standard B-form. The conformation of DNA-DNA duplex segment belongs to B-form. The hybrid-DNA duplex junction shows a structural discontinuities, A-B junction. The same conformational characteristic of oligo(dA): oligo(dT) tract as that of DNA oligomer which has same base sequence has been observed.
Fujii
S
S
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Matsui
S
S
Tomita
K
K
Orita
M
M
Tanaka
T
T
Uesugi
S
S
D001483:Base Sequence; D004247:DNA; D004261:DNA Replication; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides; D012996:Solutions
C022262:Okazaki fragments; D009838:Oligodeoxyribonucleotides; D012996:Solutions; D004247:DNA