Purification of TEM-1 beta-lactamase by immunoaffinity chromatography.
A monoclonal antibody prepared against TEM-1 beta-lactamase was found to compete with penicillins and cephalosporins for binding to the enzyme. The purified antibody preparation was linked to Sepharose 4B and used for immunoaffinity-chromatography purification of TEM-1 beta-lactamase. Elution with either benzylpenicillin or cloxacillin yielded a highly purified, concentrated and active enzyme preparation.
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Department of Molecular Biology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D002846:Chromatography, Affinity / Q000379:methods; D003023:Cloxacillin / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D010400:Penicillin G / Q000302:isolation & purification; D001618:beta-Lactamases / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D001618:beta-Lactamases; C034413:beta-lactamase TEM-1; D003023:Cloxacillin; D010400:Penicillin G