Role of protein kinase C in U46619-induced platelet shape change, aggregation and secretion.
Stimulation of rat platelets with U46619 induced Ca2+ mobilization and platelet shape change, but aggregation and secretion were induced when platelets were stimulated with U46619 plus phorbol 12-myristate 13-acetate (PMA). However, stimulation of rabbit platelets with U46619 induced all the three platelet responses. Aggregation and secretion of rabbit platelets were enhanced by simultaneous addition of PMA and inhibited by staurosporine, but platelet shape change was not affected by them. These results suggest that protein kinase C is important for aggregation and secretion, but not for platelet shape change. On the other hand, pretreatment of platelets with PMA inhibited platelet shape change as well as Ca2+ mobilization and inositol phosphate formation, indicating that the shape change was mediated by PMA-sensitive mechanism which was not clarified.
Nakano
T
T
Shionogi Research Laboratories, Shionogi & Co., Ltd., Osaka, Japan.
Hanasaki
K
K
Arita
H
H
D019796:15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid; D000255:Adenosine Triphosphate / Q000378:metabolism; D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D002118:Calcium / Q000097:blood; D066298:In Vitro Techniques; D010974:Platelet Aggregation / Q000187:drug effects; D011450:Prostaglandin Endoperoxides, Synthetic / Q000494:pharmacology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000097:blood*; D011817:Rabbits; D051381:Rats; D019311:Staurosporine; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000470:Alkaloids; D011450:Prostaglandin Endoperoxides, Synthetic; D019796:15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid; D000255:Adenosine Triphosphate; D011493:Protein Kinase C; D019311:Staurosporine; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium