title: New approach to eliminate HLA class I antigens from platelet surface without cell damage: acid treatment at pH 3.0.
abstract: A new method was studied for eliminating HLA class I antigens from the surface of platelets without damaging the cells. Platelets were exposed to an acid solution (pH 3.0) to eliminate the antigenicity of HLA class I antigens. The reduction in antigenicities of HLA class I common antigen and individual HLA class I antigens by acid treatment was marked. Patients' sera which contained multispecific HLA antibodies reacted with PBS-treated platelets, but not with acid-treated platelets. No changes were observed in the antigenicities of glycoprotein Ib or glycoprotein IIb/IIIa. The viability of acid-treated platelets was 83%. Ultrastructural investigations revealed no significant difference between the PBS-treated platelets and acid-treated platelets. The platelet function studies showed that the aggregation of acid-treated platelets induced by various agonists was only slightly reduced compared with PBS-treated platelets. We propose that acid-treated platelets are promising for clinical use in patients refractory to platelet transfusions and may be superior to chloroquine-treated platelets for analysis of the specificity of antiplatelet antibodies.
authors.affiliation: Department of Blood Transfusion, Osaka University Hospital, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kurata
authors.forename: M
authors.initials: M
authors.lastname: Oshida
authors.forename: H
authors.initials: H
authors.lastname: Take
authors.forename: T
authors.initials: T
authors.lastname: Furubayashi
authors.forename: H
authors.initials: H
authors.lastname: Nakao
authors.forename: Y
authors.initials: Y
authors.lastname: Tomiyama
authors.forename: Y
authors.initials: Y
authors.lastname: Kanayama
authors.forename: N
authors.initials: N
authors.lastname: Nagao
authors.forename: Y
authors.initials: Y
authors.lastname: Okubo
authors.forename: T
authors.initials: T
authors.lastname: Yonezawa
mesh_terms: D001792:Blood Platelets / Q000032:analysis / Q000187:drug effects* / Q000276:immunology; D002470:Cell Survival / Q000187:drug effects; D055598:Chemical Phenomena; D002621:Chemistry; D002951:Citrates / Q000494:pharmacology*; D019343:Citric Acid; D006680:HLA Antigens / Q000032:analysis / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000032:analysis / Q000276:immunology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008562:Membrane Glycoproteins / Q000032:analysis / Q000276:immunology
chemical_list: D002951:Citrates; D006680:HLA Antigens; D015395:Histocompatibility Antigens Class I; D008562:Membrane Glycoproteins; D019343:Citric Acid