title: Clinical application of a new enzyme-linked assay for the estimation of brain-specific creatine kinase in head injured patients.
abstract: The clinical value of serum brain specific creatine kinase (CK-BB) was assessed in head injured patients (group A) using a new enzyme-linked immunosorbent assay (ELISA). The results were compared to healthy controls (group B) and patients post-myocardial infarction (group C). None of the head injured patients had undergone a surgical procedure or ventricular puncture. CK-BB was significantly higher in group A than in controls. The level of CK-BB in group A was inversely proportional to the Glasgow Coma Scale on admission. All patients with a CK-BB greater than 100 micrograms/l died. The ELISA technique is a simple and reliable assay with prognostic significance in patients with head injury and has wider clinical application than the previously described radioimmunoassay methods.
authors.affiliation: Department of Surgery, Leicester Royal Infirmary.
authors.forename: P
authors.initials: P
authors.lastname: Curley
authors.forename: R
authors.initials: R
authors.lastname: Abbott
authors.forename: D
authors.initials: D
authors.lastname: Vallance
mesh_terms: D001930:Brain Injuries / Q000150:complications / Q000201:enzymology* / Q000401:mortality; D003402:Creatine Kinase / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D012887:Skull Fractures / Q000201:enzymology*
chemical_list: D003402:Creatine Kinase