title: Calibration of 125I-polymer standards with 125I-brain paste standards for use in quantitative receptor autoradiography.
abstract: 125I-Polymer standards were calibrated by interpolation of their optical densities in [125I]-brain paste standard curves to obtain dpm/mg protein. There was a linear relationship between the calibrated polymer standards and the dpm/mg polymer, as provided by the manufacturer. One dpm/mg polymer was equivalent to 7.34 +/- 0.22 dpm/mg protein. Receptor quantification in selected rat brain areas with comparison to either brain paste or calibrated polymer standards yielded similar results.
authors.affiliation: Section on Pharmacology, National Institute of Mental Health, Bethesda, MD 20892.
authors.forename: A J
authors.initials: AJ
authors.lastname: Nazarali
authors.forename: J S
authors.initials: JS
authors.lastname: Gutkind
authors.forename: J M
authors.initials: JM
authors.lastname: Saavedra
mesh_terms: D000818:Animals; D001345:Autoradiography / Q000592:standards*; D001921:Brain / Q000378:metabolism*; D007457:Iodine Radioisotopes / Q000592:standards*; D011108:Polymers / Q000592:standards*; D051381:Rats
chemical_list: D007457:Iodine Radioisotopes; D011108:Polymers