[Detection of deletions by the amplification of exons (multiplex PCR) in Duchenne muscular dystrophy].
The polymerase chain reaction is a new powerful method for in vitro cloning of specific regions of DNA. The use of the heat-stable DNA polymerase made the reaction amenable to automation. This method greatly facilitates the detection of mutations which are responsible for Duchenne muscular dystrophy, via DNA amplification of multiple deletions prone exons from the DMD gene.
Claustres
M
M
Kjellberg
P
P
Desgeorges
M
M
Bellet
H
H
Sarda
P
P
Bonnet
H
H
Boileau
C
C
D002872:Chromosome Deletion; D006801:Humans; D009136:Muscular Dystrophies / Q000175:diagnosis* / Q000235:genetics; D016133:Polymerase Chain Reaction; D011296:Prenatal Diagnosis; D012732:Sex Determination Analysis; D014960:X Chromosome
Détéction des délétions par amplification d'exons (PCR multiplex) dans la myopathie de Duchenne.