[A method for the determination of cytosolic aminopeptidase in serum and its usefulness for clinical application. Selection of inhibitors for membrane-bound aminopeptidase and cystyl aminopeptidase].
A method for the determination of cytosolic aminopeptidase (EC 3.4.11.1; C-AP) in serum was developed. At first, more specific and adequate inhibitor for other serum peptidases, such as membrane-bound aminopeptidase (EC 3.4.11.2; arylamidase, AA) and cystyl aminopeptidase (EC 3.4.11.3; CAP) was selected from 1,10-phenanthroline derivatives. The compound, 4,7-dimethyl-1,10-phenanthroline (4,7-DMP) is one of the most effective inhibitor for AA and CAP, and it inhibits completely these enzymes at the concentration of less than 0.4 mmol/l. C-AP in serum at an optimum pH of 8.0 in the assay using L-leucine amide (LA) as the substrate had no inhibitory effects at the concentration of more than 0.4 mmol/l of 4,7-DMP. The results with the proposed method correlated with those with a conventional electrophoretic method. The proposed method hence is a specific and easily available assay for C-AP in serum. The further analysis of C-AP using this method would promise the establishment of clinical assessment of the enzyme.
Taniguchi
K
K
Sugiyama
M
M
Shimizu
H
H
Azuma
Y
Y
Kanno
T
T
D000626:Aminopeptidases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D010122:Cystinyl Aminopeptidase / Q000037:antagonists & inhibitors*; D006801:Humans; D007931:Leucyl Aminopeptidase / Q000097:blood*; D010618:Phenanthrolines
D010618:Phenanthrolines; D000626:Aminopeptidases; D007931:Leucyl Aminopeptidase; D010122:Cystinyl Aminopeptidase