title: Effect of 1 alpha,25-dihydroxyvitamin D3 on polyamine metabolism in human monocyte cell line-U937.
abstract: 1. Pretreatment with 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] caused an increase in ornithine decarboxylase (EC 4.1.1.17) (ODC) activity in lipopolysaccharide (LPS)-stimulated human monocyte cell line, U937. 2. The increase in ODC activity was dose-dependent and preincubation-time dependent. 3. 26,26,26,27,27,27-Hexafluoro-1,25-dihydroxyvitamin D3 was ca 7 times more potent than 1,25-(OH)2D3 in increasing ODC activity. 4. Pretreatment with 1,25-(OH)2D3 also potentiated the activity of spermidine/spermine-N1-acetyltransferase in LPS-stimulated U937 cells. 5. Putrescine levels in cells pretreated with 1,25-(OH)2D3 increased ca 2-fold 4-8 hr after LPS addition. 6. However, pretreatment with 1,25-(OH)2D3 did not cause any increase in ODC mRNA level, suggesting that 1,25-(OH)2D3 may modulate polyamine metabolism at the posttranscriptional level rather than the transcriptional step.
authors.affiliation: Second Department of Internal Medicine, Osaka City University Medical School, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yukioka
authors.forename: S
authors.initials: S
authors.lastname: Otani
authors.forename: I
authors.initials: I
authors.lastname: Matsui-Yuasa
authors.forename: H
authors.initials: H
authors.lastname: Goto
authors.forename: H
authors.initials: H
authors.lastname: Tahara
authors.forename: S
authors.initials: S
authors.lastname: Morisawa
authors.forename: S
authors.initials: S
authors.lastname: Okuno
authors.forename: Y
authors.initials: Y
authors.lastname: Nishizawa
authors.forename: H
authors.initials: H
authors.lastname: Morii
mesh_terms: D000123:Acetyltransferases / Q000378:metabolism; D002117:Calcitriol / Q000494:pharmacology*; D002460:Cell Line; D004357:Drug Synergism; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D009955:Ornithine Decarboxylase / Q000096:biosynthesis / Q000235:genetics; D011073:Polyamines / Q000378:metabolism*; D011700:Putrescine / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D000099228:Diamine N-Acetyltransferase
chemical_list: D000123:Acetyltransferases; D002117:Calcitriol; D008070:Lipopolysaccharides; D009955:Ornithine Decarboxylase; D011073:Polyamines; D011700:Putrescine; D012333:RNA, Messenger; D000099228:Diamine N-Acetyltransferase