Method for rapid separation of 3,5,3'-triiodothyroacetic acid in human serum by fast protein liquid chromatography.
The 3,5,3'-triiodothyroacetic acid (TRIAC) has been approved as a valuable agent in the management of hyperthyroidism secondary to inappropriate secretion of thyrotropin. We have developed a fast protein liquid chromatography (FPLC) method for separation and quantification of TRIAC. Serum samples charged with TRIAC were extracted with methanol/ammonium acetate, the supernatants were evaporated to dryness, reconstituted in NaOH and injected on a reversed phase column for chromatography. For separation an isocratic elution method (methanol water; 0.1% trifluoroacetic acid) was used. The area under the curve (ml%) was compared with those of the calibration curves. Recoveries were 70 +/- 10.8%. TRIAC was eluted in 2.33 ml. Conclusively, the present method shows that TRIAC can be measured by FPLC and may be applied to the measurement of TRIAC in pharmacological studies.
Duntas
L
L
Department of Internal Medicine I, University Clinic of Ulm, FRG.
Keck
F S
FS
Wolf
C
C
Pfeiffer
E F
EF
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D013974:Thyroxine / Q000097:blood; D014284:Triiodothyronine / Q000031:analogs & derivatives* / Q000097:blood
D014284:Triiodothyronine; C010642:3,3',5-triiodothyroacetic acid; D013974:Thyroxine