The effects of anesthetics on the concentrations of cholecystokinin octapeptide sulfate-like immunoreactivity in rat brain regions.
Cholecystokinin octapeptide sulfate-like immunoreactivity (CCK-8S-LI) was determined by radioimmunoassay in rat brain areas following injections of pentobarbital, halothane and chloral hydrate. Time-dependent changes in the concentrations of CCK-8S-LI were different between pentobarbital and chloral hydrate in all brain regions studied. After pentobarbital injection, CCK-8S-Li peaked at 30-60 min in the frontal cortex, nucleus accumbens, striatum and substantia nigra; after chloral hydrate injection, CCK-8S-LI peaked at 120 min in the hypothalamus, nucleus accumbens and substantia nigra. Both anesthetics induced almost the same sleeping times. Halothane inhalation caused increases in the concentrations of CCK-8S-LI in the amygdala and hippocampus. In addition, following intracardial perfusion of saline for 30 min after pentobarbital anesthesia, the concentrations of CCK-8S-LI increased in the nucleus accumbens, and decreased in the frontal cortex. These results suggest that since different anesthetics cause different changes in CCK levels, anesthetics affect studies of these neurons.
Kushima
Y
Y
Department of Life Chemistry, Tokyo Institute of Technology, Yokohama, Japan.
Takeda
K
K
Oh-Hashi
Y
Y
Nakagawa
T
T
Kato
T
T
D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects*; D002697:Chloral Hydrate / Q000494:pharmacology*; D004347:Drug Interactions; D006221:Halothane / Q000494:pharmacology*; D006321:Heart; D008297:Male; D010424:Pentobarbital / Q000494:pharmacology*; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D012844:Sincalide / Q000032:analysis*; D012965:Sodium Chloride / Q000008:administration & dosage / Q000494:pharmacology; D013997:Time Factors
D002697:Chloral Hydrate; D012965:Sodium Chloride; D010424:Pentobarbital; D012844:Sincalide; D006221:Halothane