Complete sequence of the coding region of human elongation factor 2 (EF-2) by enzymatic amplification of cDNA from human ovarian granulosa cells.
The use of two primers allowed the specific enzymatic amplification of elongation factor 2 starting with total double-stranded cDNA from human ovarian granulosa cells. The amplified DNA fragment with a length of 1765 bp was restricted and sequenced by the shot gun approach. From the sequences obtained from the amplified fragment and the cDNA insert of pHGR81 [Rapp et al. (1988) Biol. Chem. Hoppe-Seyler 369, 247-250] respectively, the DNA sequence containing the complete coding as well as the 3'-untranslated region was assembled.
Rapp
G
G
Max-Planck-Institut für Biophysikalische Chemie, Abt. Molekulare Biologie, Göttingen.
Klaudiny
J
J
Hagendorff
G
G
Luck
M R
MR
Scheit
K H
KH
D000818:Animals; D001483:Base Sequence; D006224:Cricetinae; D004247:DNA / Q000032:analysis*; D005260:Female; D006107:Granulosa Cells / Q000032:analysis*; D006801:Humans; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors / Q000235:genetics*; D051381:Rats; D012689:Sequence Homology, Nucleic Acid
D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors; D004247:DNA