title: Radiation-induced changes of fibroblasts in the squamous cell carcinoma of the head and neck.
abstract: The regrowth of mesenchymal tissue (stroma) surrounding the malignant epithelium is an important step in tissue remodelling during and after irradiation. The radiation-induced fibroblastic changes were studied on tissue samples taken before, during and after the radical irradiation of the squamous cell carcinoma of the head and neck. Elongated fibroblasts with large amount of rough endoplasmic reticulum were seen around the tumor epithelium before radiation. The fibrosis increased during irradiation and at the same time the shape of the fibroblasts changed so that they became more triangular and nuclear structures became more prominent together with hyperchromasia. The amount of cell organelles declined although there was a large amount collagen present. Epithelial cells invaded through the basal lamina. In most samples the basal lamina could not be seen at all and the tumor cells were dispersed between stromal elements. On the other hand there were close contacts between epithelial and mesenchymal cells throughout the study in places where the basal lamina was broken, which might indicate epithelio-mesenchymal interaction. Also the connective tissue formed by fibroblasts and collagen might be part of the radiation induced healing and destruction of the tumor cells.
authors.affiliation: Department of Radiotherapy, University of Turku, Finland.
authors.forename: P
authors.initials: P
authors.lastname: Kellokumpu-Lehtinen
authors.forename: K O
authors.initials: KO
authors.lastname: Söderström
authors.forename: A
authors.initials: A
authors.lastname: Kortekangas
authors.forename: E
authors.initials: E
authors.lastname: Nordman
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000532:radiotherapy* / Q000648:ultrastructure; D006258:Head and Neck Neoplasms / Q000532:radiotherapy* / Q000648:ultrastructure; D006801:Humans; D008875:Middle Aged