Effect of agitation rate and impeller design on oxygen transfer coefficients in small bioreactors using surface aeration.
The effects of agitation rate and impeller type on the combined oxygen mass-transfer coefficient (kL a) in four different benchtop bioreactors have been examined. Surface oxygenation of a cell culture medium supplemented with fetal bovine serum and distilled deionized water has been studied by passing air through the bioreactor headspace at approximately one headspace volume per minute. A new ribbon-type impeller design using strips of Teflon has been shown to be superior to conventional impeller designs for oxygen transfer.
Sybert
E M
EM
Engineering Research Center, University of Maryland, College Park 20742.
Winter
E L
EL
Cadman
T W
TW
Conner
M A
MA
D001709:Biotechnology; D002478:Cells, Cultured; D003470:Culture Media; D003627:Data Interpretation, Statistical; D010100:Oxygen; D014867:Water
D003470:Culture Media; D014867:Water; D010100:Oxygen