title: Na+ and K+ concentration and regulation of protein synthesis in L-A9 and Aedes albopictus cells infected with Marituba virus (Bunyaviridae).
abstract: Infection of L-A9 cells with Marituba virus produces a severe inhibition of protein synthesis. This inhibition is temporally correlated with an increase in the intracellular Na+ concentration and a decrease in the intracellular K+ concentration. However, in Marituba virus-infected Aedes albopictus cells the intracellular level of Na+ and K+ ions and protein synthesis remained unaltered. Incubation of both cell types at high NaCl concentration facilitated the translation of viral RNA whereas the cellular protein synthesis was inhibited. Using a hypotonic medium, the opposite was found. Results are discussed in terms of a possible involvement of these ions in the viral translational process.
authors.affiliation: Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Cidade Universitária, Brasil.
authors.forename: I C
authors.initials: IC
authors.lastname: Frugulhetti
authors.forename: M A
authors.initials: MA
authors.lastname: Rebello
mesh_terms: D000330:Aedes; D000818:Animals; D001345:Autoradiography; D002042:Bunyamwera virus / Q000235:genetics / Q000502:physiology*; D002043:Bunyaviridae / Q000502:physiology*; D002460:Cell Line; D002999:Clone Cells; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D051379:Mice; D011188:Potassium / Q000378:metabolism*; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000235:genetics; D012964:Sodium / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D003470:Culture Media; D012367:RNA, Viral; D012964:Sodium; D011188:Potassium