Radioimmunoassay to determine postprandial changes in plasma neuropeptide Y levels in awake dogs.
A specific, precise and sensitive double-antibody radioimmunoassay for Neuropeptide Y (NPY) has been developed. There was no appreciable cross-reactivity with the structurally related peptides, peptide YY (PYY) and pancreatic polypeptide (PP). The minimum detectable plasma NPY level was 3 nM. Application of radioimmunoassay to canine models revealed that portal and systemic NPY levels increased significantly following a standard meal.
Balasubramaniam
A
A
Department of Surgery, University of Cincinnati Medical Center, Ohio 45267.
McFadden
D W
DW
Rudnicki
M
M
Nussbaum
M S
MS
Dayal
R
R
Srivastava
L S
LS
Fischer
J E
JE
D000818:Animals; D003429:Cross Reactions; D004285:Dogs; D004435:Eating; D009478:Neuropeptide Y / Q000097:blood* / Q000378:metabolism / Q000592:standards; D011863:Radioimmunoassay / Q000379:methods*
D009478:Neuropeptide Y