A petite positive strain of Kluyveromyces lactis has a 300 kb deletion in the rDNA cluster.
By employing Pulsed Field Gel Electrophoresis we have shown that the chromosomal DNA pattern of a petite-positive strain of Kluyveromyces lactis, designated KF4, differs from the karyotype of the reference strain. Digestion with SfiI and hybridization with an rDNA probe, demonstrate that a novel chromosomal band in KF4 results from a deletion of 35-40 rDNA cistrons from the rDNA cluster. The significance of this finding to possible alterations in cell physiology and the ability to generate "petite" mutants is discussed.
Maleszka
R
R
Molecular and Population Genetics Group, Research School of Biological Sciences, Australian National University, Canberra, ACT.
Clark-Walker
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GD
D015139:Blotting, Southern; D002872:Chromosome Deletion; D004271:DNA, Fungal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics*; D007621:Karyotyping; D007716:Kluyveromyces / Q000235:genetics*; D010641:Phenotype; D012091:Repetitive Sequences, Nucleic Acid; D004718:Saccharomycetales / Q000235:genetics*
D004271:DNA, Fungal; D004275:DNA, Ribosomal