Adaptation of the Nelson-Somogyi reducing-sugar assay to a microassay using microtiter plates.
The Nelson-Somogyi assay for reducing sugars was adapted to microtiter plates. The primary advantages of this modified assay are (i) smaller sample and reagent volumes, (ii) elimination of boiling and filtration steps, (iii) automated measurement with a dual-wavelength scanning TLC densitometer, (iv) increased range and reproducibility, and (v) automated colorimetric readings by reflectance rather than absorbance.
Green
F
F
USDA Forest Service, Forest Products Laboratory, Madison, Wisconsin 53705-2398.
Clausen
C A
CA
Highley
T L
TL
D000818:Animals; D002241:Carbohydrates / Q000032:analysis*; D002417:Cattle; D002480:Cellulase; D002855:Chromatography, Thin Layer / Q000379:methods; D003720:Densitometry / Q000379:methods; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006026:Glycoside Hydrolases / Q000032:analysis / Q000378:metabolism; D008832:Microchemistry / Q000379:methods*; D012015:Reference Standards; D015203:Reproducibility of Results; D043325:Xylan Endo-1,3-beta-Xylosidase
D002241:Carbohydrates; D006026:Glycoside Hydrolases; D043325:Xylan Endo-1,3-beta-Xylosidase; D004693:Glucan Endo-1,3-beta-D-Glucosidase; D002480:Cellulase; C019826:carboxymethylcellulase; D005947:Glucose