A fast atom bombardment-mass spectrometric method to quantitate lysophosphatidylserine in rat brain.
A method to quantitate lysophosphatidylserine by fast atom bombardment-mass spectrometry using 1-hexa-decanoyl-sn-glycero-3-phospho-L-serine as internal standard is described. The standard curve is linear with a correlation coefficient r2 = 0.999 from 10 to 1000 ng. This curve has been used to quantitate LPS in rat brain using phosphorus assay as a test control. We found 475 +/- 70 ng of LPS in 1 mg of tissue (n = 3). This method presents advantages due to its sensitivity and its capability to give molecular information of the unmodified compound.
Benfenati
E
E
Istituto di Ricerche Farmacologiche Mario Negri, Bergamo, Italy.
De Bellis
G
G
Chen
S
S
Bettazzoli
L
L
Fanelli
R
R
Tacconi
M T
MT
Kirschner
G
G
Pege
G
G
D000818:Animals; D002540:Cerebral Cortex / Q000032:analysis*; D008246:Lysophospholipids / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods; D051381:Rats
D008246:Lysophospholipids; C025059:lysophosphatidylserine