title: [Comparative study on human chorionic gonadotropin receptor in normal human ovary with ovarian tumors].
abstract: Human Chorionic Gonadotropin (HCG) is major physiological luteotropic factors for the human corpus luteum. The observations strongly suggest that the human ovary possesses a gonadotropin receptor in the cell membrane. We studied the HCG receptor in normal human ovary and ovarian tumors. Twenty-three human ovarian specimens and 16 ovarian tumor specimens were obtained from women patients having gynecological surgery. Ovaries were homogenized and sonicated. The homogenates were centrifuged at 2000 g for 15 min. After sucrose density gradient ultracentrifugation (78,000 g, 4 h), two fractions were collected from layer of 33% and interface between 33% and 37%. Thirty micrograms of ovarian protein, 8 ng 125I-HCG and unlabeled HCG in a final volume of 0.5 ml of 0.05 mol/L Tris buffer were incubated at 30 degrees C for 2 h. The results were shown in the table.
authors.forename: M L
authors.initials: ML
authors.lastname: Chen
authors.forename: Z L
authors.initials: ZL
authors.lastname: Du
authors.forename: T H
authors.initials: TH
authors.lastname: Lan
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Cao
authors.forename: S W
authors.initials: SW
authors.lastname: Han
authors.forename: Y D
authors.initials: YD
authors.lastname: Lei
authors.forename: L
authors.initials: L
authors.lastname: Li
mesh_terms: D001665:Binding Sites; D003536:Cystadenocarcinoma / Q000032:analysis*; D005260:Female; D006801:Humans; D010051:Ovarian Neoplasms / Q000032:analysis*; D010053:Ovary / Q000032:analysis*; D011967:Receptors, Gonadotropin / Q000032:analysis* / Q000378:metabolism
chemical_list: D011967:Receptors, Gonadotropin