title: [Effects of monoclonal antibodies to bovine nerve growth factor on NGF-induced cell differentiation in culture].
abstract: Five Hybridoma clones producing monoclonal antibodies (MAT) to bovine nerve growth factor (NGF) were developed. The biological effects of antibodies were studied: the influence of MAT on neurit outgrowth induced by NGF in rat pheochromocytoma PC12 or spinal chicken ganglia was investigated. MAT fell into two groups. Two of them inhibited neurit induction by NGF, three others stimulated this process. The stimulation of the neurit outgrowth by MAT was observed at low concentration of NGF (3 ng/ml of culture medium). Mechanisms of antibodies effects are discussed.
authors.forename: E S
authors.initials: ES
authors.lastname: Kakpakova
authors.forename: E M
authors.initials: EM
authors.lastname: Malakhova
authors.forename: S N
authors.initials: SN
authors.lastname: Gnedoĭ
authors.forename: T V
authors.initials: TV
authors.lastname: Pochechueva
authors.forename: I A
authors.initials: IA
authors.lastname: Filatov
authors.forename: S M
authors.initials: SM
authors.lastname: Dudkin
authors.forename: A A
authors.initials: AA
authors.lastname: Stavrovskaia
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D003470:Culture Media; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D009414:Nerve Growth Factors / Q000276:immunology* / Q000494:pharmacology
chemical_list: D000911:Antibodies, Monoclonal; D003470:Culture Media; D009414:Nerve Growth Factors
vernacular_title: Vliianie monoklonal'nykh antitel k faktoru rosta nervov byka na FRN-indutsirovannuiu differentsirovku kletok v kul'ture.