[Studies on protective monoclonal antibodies to Leishmania donovani].
Kala-azar is increasing in incidence in some endemic areas of China, such as provinces of Sichuan and Gansu. Monoclonal antibodies have been shown to interfere with the cutaneous leishmaniasis infection in the experimental animals. We have now raised McAb against membrane antigen of promastigotes of L. donovani canine isolate (the pathogen of visceral leishmaniasis). In this paper we report 3 McAb which inhibit the growth of promastigotes in vitro culture by incorporation of 3H-thymine deoxyriboside, such as McAb 2B12-A8, 2H6-E3, in the presence of complement, showing strong inhibiting effects with a decrease in incorporation of 3H-TdR, 94-99.3% rate of inhibition, whereas the inhibition rate induced by McAb 1B1-C2 fluctuated in a range of 68-95%, which is identical with the results of macrophage infection inhibition test by the same McAb, 61-87%. The results of ultrastructural localization of L. donovani antigen by protective McAb 2H6-E3 with immunogold technique showed the gold particles, in clumps, widely distributed on the outer side of promastigotes membrane. According to the high density of localized gold particles, it is suggested that this antigen is abundant on the plasma membrane.
Hu
X S
XS
Lin
F Q
FQ
Wang
L
L
Lieu
P N
PN
Kan
B
B
Wang
Y J
YJ
Wang
Z L
ZL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000953:Antigens, Protozoan / Q000276:immunology*; D007893:Leishmania donovani / Q000254:growth & development / Q000276:immunology*; D008264:Macrophages; D051379:Mice; D008807:Mice, Inbred BALB C; D010587:Phagocytosis
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan