title: Estimation of DNA content in uveal melanomas by flow cytometry.
abstract: Flow cytometry was used to evaluate ploidy and tumour cycle kinetics in fresh tissue samples obtained from 19 uveal melanomas. The results were compared with other parameters including, histological cell type, tumour size and anatomical location. Three tumours (15.8%) were aneuploid (two mixed cell, one epithelioid cell). Cell turnover was estimated in the 16 diploid tumours by summating the total percentage of cells in S and G2/M phases. We found the mean percentage of cells in G2/M/S to be 5.96% (range 2.2-9.8%). Spindle cell neoplasms appeared to have lower cell turnover rates (4.5 +/- 1.2%) than epithelioid cell turnover (8.4 +/- 1.2%). There was no correlation between cell turnover and either tumour size or anatomical location.
authors.affiliation: Department of Ophthalmology, University of Sheffield Medical School, Royal Hallamshire Hospital.
authors.forename: I G
authors.initials: IG
authors.lastname: Rennie
authors.forename: R C
authors.initials: RC
authors.lastname: Rees
authors.forename: M A
authors.initials: MA
authors.lastname: Parsons
authors.forename: J
authors.initials: J
authors.lastname: Lawry
authors.forename: D
authors.initials: D
authors.lastname: Cottam
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002453:Cell Cycle; D002455:Cell Division; D002469:Cell Separation; D002830:Choroid Neoplasms / Q000032:analysis / Q000235:genetics* / Q000473:pathology; D002924:Ciliary Body / Q000032:analysis* / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008545:Melanoma / Q000032:analysis / Q000235:genetics* / Q000473:pathology; D008875:Middle Aged; D011446:Prospective Studies; D014604:Uveal Neoplasms / Q000032:analysis / Q000235:genetics* / Q000473:pathology
chemical_list: D004273:DNA, Neoplasm