Detection of early pregnancy factor (EPF) in pregnant and nonpregnant subjects with the rosette inhibition test.
We tested for early pregnancy factor (EPF) using a rosette inhibition test with polyclonal anti-lymphocyte serum from the horse and two monoclonal antibodies specific for the E-receptor of T-lymphocytes. When lymphocytes were preincubated with early human pregnancy sera, rosette inhibition titres were four or more dilutions higher (P less than 0.01) than when lymphocytes were preincubated with nonpregnant sera.
Straube
W
W
Klinik für Gynäkologie und Geburtshilfe, Ernst-Moritz-Arndt-Universität, Griefswald, GDR.
Tiemann
U
U
Loh
M
M
Schütz
M
M
D000911:Antibodies, Monoclonal; D000961:Antilymphocyte Serum / Q000032:analysis; D018835:Chaperonin 10; D005260:Female; D006801:Humans; D007166:Immunosuppressive Agents / Q000032:analysis*; D010455:Peptides / Q000032:analysis*; D011247:Pregnancy; D011257:Pregnancy Proteins; D011259:Pregnancy Tests, Immunologic; D012397:Rosette Formation / Q000379:methods*; D013491:Suppressor Factors, Immunologic; D013997:Time Factors
D000911:Antibodies, Monoclonal; D000961:Antilymphocyte Serum; D018835:Chaperonin 10; D007166:Immunosuppressive Agents; D010455:Peptides; D011257:Pregnancy Proteins; D013491:Suppressor Factors, Immunologic; C025124:early pregnancy factor