Absence of a lyso-PAF relationship with PAF (platelet activating factor) in monosodium urate crystal-induced inflammatory exudates.
Monosodium urate crystal (MSU)-induced inflammation was evaluated for increased vascular permeability and platelet activating factor (PAF) production. Intraperitoneal injection of MSU crystals increased vascular permeability and lyso-PAF, as shown by acetylation and bioassay, but PAF was not detected. Intraperitoneal administration of PAF elicited dye-extravasation, which was abolished by the PAF antagonist WEB-2086, but this compound had no significant effect upon MSU-induced dye extravasation. Thus, the presence of lyso-PAF did not reflect in situ production of PAF. Lyso-PAF extracted from normal serum in amounts equivalent to that extravasated could account for the lyso-PAF observed. Thin-layer chromatographic analysis of acetylated extracts from plasma and peritoneal washouts showed identical Rf values to the PAF standard. We conclude that lyso-PAF in the exudate was associated with extravasated plasma proteins rather than a reflection of in situ production of PAF.
Griswold
D E
DE
Department of Immunology, SK&F Laboratories, Swedeland, PA 19479.
Hillegass
L
L
Rotilio
D
D
Mong
S
S
Hanna
N
N
D000818:Animals; D001202:Ascitic Fluid / Q000473:pathology / Q000503:physiopathology; D002199:Capillary Permeability / Q000502:physiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010538:Peritonitis / Q000139:chemically induced / Q000503:physiopathology*; D010972:Platelet Activating Factor / Q000031:analogs & derivatives* / Q000502:physiology*; D014527:Uric Acid
C029271:O-deacetyl platelet activating factor; D010972:Platelet Activating Factor; D014527:Uric Acid