title: [Hairy cell leukemia: study of genomic rearrangements in splenic infiltration].
abstract: Genomic DNA extracted from heavily infiltrated spleens of 5 patients with hairy cell leukaemia was hybridized with genomic probes for immunoglobulin genes. The rearrangement pattern, correlating well with the immunohistochemical findings, was that of a clonal B-cell proliferation in all cases. Analysis of the lambda light chain genome with two different probes enabled more accurate localization of the clonal rearrangements within this genome. A small additional T-cell population identified in 3 cases was immunogenotypic of polyclonal (reactive) nature.
authors.affiliation: Département de médecine interne, CHUV, Lausanne.
authors.forename: H
authors.initials: H
authors.lastname: Knecht
authors.forename: B F
authors.initials: BF
authors.lastname: Odermatt
authors.forename: D
authors.initials: D
authors.lastname: Hayoz
authors.forename: F
authors.initials: F
authors.lastname: Bachmann
mesh_terms: D015342:DNA Probes; D004273:DNA, Neoplasm / Q000235:genetics; D015328:Gene Rearrangement, B-Lymphocyte, Light Chain / Q000235:genetics*; D015329:Gene Rearrangement, T-Lymphocyte / Q000235:genetics; D006801:Humans; D007943:Leukemia, Hairy Cell / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D013154:Spleen / Q000166:cytology
chemical_list: D015342:DNA Probes; D004273:DNA, Neoplasm
vernacular_title: Leucémie à tricholeucocytes (hairy cell leukaemia): étude des réarrangements génomiques de l'infiltration splénique.