title: 18-substituted steroids--Part 17. 2 alpha-hydroxylated liver metabolites of aldosterone identified by high-field [1H]NMR spectroscopy.
abstract: 11 beta,18-Epoxy-2 alpha,3 alpha,18,21-tetrahydroxy-5 alpha,17 alpha- pregnan-20-one (2 alpha-hydroxy-3 alpha,5 alpha-tetrahydro-17-isoaldosterone) and its apo isomer have been identified by high-field NMR studies, supported by thermospray HPLC/MS, to be among the major polar metabolites formed from incubation of aldosterone with rat liver microsomal fraction. Indications that unreduced 2 alpha-hydroxy-aldosterone is also present among the metabolites have still to be confirmed.
authors.affiliation: Department of Laboratory Medicine, Miriam Hospital, Providence, Rhode Island.
authors.forename: S A
authors.initials: SA
authors.lastname: Latif
authors.forename: D J
authors.initials: DJ
authors.lastname: Morris
authors.forename: L
authors.initials: L
authors.lastname: Wei
authors.forename: D N
authors.initials: DN
authors.lastname: Kirk
authors.forename: P J
authors.initials: PJ
authors.lastname: Burke
authors.forename: H C
authors.initials: HC
authors.lastname: Toms
authors.forename: C H
authors.initials: CH
authors.lastname: Shackleton
mesh_terms: D000450:Aldosterone / Q000378:metabolism*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D013058:Mass Spectrometry; D008862:Microsomes, Liver / Q000378:metabolism*; D011278:Pregnanes / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D011278:Pregnanes; C062591:11,18-epoxy-2,3,18,21-tetrahydroxypregnan-20-one; D000450:Aldosterone