A simple procedure for the isolation of schistosomules for biochemical studies or culture.
Incubation of cercariae of Schistosoma mansoni in a 1:1 mixture of Eagle's Minimal Essential Medium and rat serum at 42 degrees C for only 5 minutes, plus subsequent vortexing, resulted in at least 98% conversion of cercariae to schistosomules. Subsequent centrifugation before or after settling for 30 minutes in an ice bath, resulted in schistosomule preparations with about 20% or 10% contamination with tail segments, respectively.
Ogbunude
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Department of Biological and Medical Research, King Faisal Specialist Hospital, Riyadh, Saudi Arabia.
Selim
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A
Haq
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Baer
H P
HP
D000818:Animals; D002498:Centrifugation; D003470:Culture Media; D010274:Parasitology / Q000379:methods; D012550:Schistosoma mansoni / Q000302:isolation & purification*
D003470:Culture Media