title: Diagnostic and prognostic significance of myelomonocytic cell surface antigens in acute myeloid leukaemia.
abstract: Thirty-six cases of acute myeloid leukaemia (AML) were tested with a large battery of monoclonal antibodies (moAbs) detecting surface markers normally expressed by myelomonocytic, T and B lymphoid, megakaryocytic and erythroid lineages. Differences in antigenic expression were observed among the various FAB subgroups: HLA-class II molecules were found in almost all AML cases but not in the promyelocytic subgroup (M3); CD14 and CD36 antigens were detected in monocytic leukaemias (M4 and M5); the CD34 moAb (MY10) recognizing an epitope described on myeloid stem cells was positive in 88% of the M1 and 80% of the M3 cases. By a multivariate analysis, only the CD14b (MY4) discriminated significantly between M1-M2 and M4-M5 subgroups. Using Cox's model to assess the prognostic importance of variables including immunophenotyping on survival, we undertook a one by one analysis and found that the presence of CD17 antigen predicted for a shorter survival (P = 0.03). In addition this marker appeared more significant than other clinical and biological parameters.
authors.affiliation: Département d'Hématologie et Unité, Claude Bernard C20, Hôpital, Pitié-Salpêtrière, Paris, France.
authors.forename: H
authors.initials: H
authors.lastname: Merle-Beral
authors.forename: L
authors.initials: L
authors.lastname: Nguyen Cong Duc
authors.forename: V
authors.initials: V
authors.lastname: Leblond
authors.forename: C
authors.initials: C
authors.lastname: Boucheix
authors.forename: A
authors.initials: A
authors.lastname: Michel
authors.forename: C
authors.initials: C
authors.lastname: Chastang
authors.forename: P
authors.initials: P
authors.lastname: Debre
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis*; D001853:Bone Marrow / Q000276:immunology; D005260:Female; D006801:Humans; D015479:Leukemia, Myelomonocytic, Acute / Q000175:diagnosis / Q000276:immunology* / Q000401:mortality; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D012737:Sex Factors
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000954:Antigens, Surface