[Production of monoclonal antibodies to human T-cell lymphotropic virus type 1 (HTLV-1) and studies of their specificity].
Mouse monoclonal antibodies to HTLV-1 core proteins, p19 and p24, were obtained by hybridoma technique. The specificity was studied by indirect immunofluorescence, enzyme-linked immunosorbent assay (ELISA), dot blot, and Western blot studies. Polyclonal antibodies reactive with HTLV-1 viral proteins were raised by rabbits and guinea pigs. A sandwich ELISA and a dot blot method using these antibodies detected the soluble viral antigens in the ATL cell lysate and ATL culture media. No reaction was observed when IL-2-activated human T-cell lysate and the culture media were used as controls. These data showed that our sandwich ELISA and dot blot systems are available for quantitative analysis of the soluble HTLV-1 viral antigens and contribute to understanding the ability of viral replication by ATL cells.
Iwatsuki
K
K
Jin
F X
FX
Takigawa
M
M
Sugaya
K
K
Yamada
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay; D006168:Guinea Pigs; D015481:HTLV-I Antibodies / Q000096:biosynthesis* / Q000276:immunology; D015486:HTLV-I Antigens / Q000032:analysis; D015368:Human T-lymphotropic virus 1 / Q000276:immunology*; D015151:Immunoblotting; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000382:microbiology; D051379:Mice; D011817:Rabbits
D000911:Antibodies, Monoclonal; D015481:HTLV-I Antibodies; D015486:HTLV-I Antigens