Isolation and characterization of human TR3 receptor: a member of steroid receptor superfamily.
Complementary DNAs (cDNAs) encoding a member of steroid receptor super-family, named TR3 receptor, were isolated from a human prostate lambda gt11 cDNA library on the basis of homology of oligonucleotide probes to the DNA-binding domain common to members of the steroid receptor super-family. Expression of TR3 receptor cDNA produced a 64 kDa DNA-binding protein in a rabbit reticulocyte lysate. Nucleotide sequence analysis showed that TR3 receptor cDNA contains two regions of sequences which correspond to the DNA- and hormone-binding domains of members of the steroid receptor super-family. The amino acid sequences in the hormone-binding domain of the TR3 receptor shares about 20% homology with estrogen receptor and less than 15% homology with other known steroid receptors. The DNA-binding domain of the TR3 receptor has about 55% homology with all other known steroid receptors. TR3 receptor had 86% nucleotide and 91% amino acid sequence homology with mouse NUR/77, suggesting that TR3 receptor may be a human homologue of mouse NUR/77 gene product.
Chang
C
C
Department of Surgery/Urology, University of Chicago, IL 60637.
Kokontis
J
J
Liao
S S
SS
Chang
Y
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000378:metabolism; D015723:Gene Library; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D005810:Multigene Family; D057105:Nuclear Receptor Subfamily 4, Group A, Member 1; D011467:Prostate / Q000378:metabolism; D011944:Receptors, Androgen / Q000235:genetics; D011987:Receptors, Steroid / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D011988:Receptors, Thyroid Hormone; D012689:Sequence Homology, Nucleic Acid; D013737:Testis / Q000378:metabolism
C539821:NR4A1 protein, human; C539818:Nr4a1 protein, mouse; D057105:Nuclear Receptor Subfamily 4, Group A, Member 1; D011944:Receptors, Androgen; D011987:Receptors, Steroid; D011988:Receptors, Thyroid Hormone; D004247:DNA