[The distribution of chlorine e6 and its derivatives in HeLa cells studied using luminescence microscopy].
The influence of the chemical structure of porphyrin pigments on their accumulation and localization in HeLa cells has been examined by the scanning fluorescence microphotometry. It has been found that the replacement of carboxyl groups of chlorine e6 for methyl and amino groups has no influence on the pigment distributions in cells. All the pigments are bound by cell membrane structures. The chemical modification of chlorine e6 structure is essential for the ability of pigment to be accumulated by cells that can be used to increase the efficiency of cancer phototherapy. The charge and hydrophobic properties of pigment molecules are of great importance for accumulating porphyrin sensitizers by cells.
Gurinovich
G P
GP
Zorina
T E
TE
Arkatov
Iu M
IuM
Sarzhevskaia
M V
MV
Cherenkevich
S N
SN
D002735:Chlorophyllides; D003592:Cytophotometry; D006367:HeLa Cells / Q000378:metabolism*; D006801:Humans; D008856:Microscopy, Fluorescence; D011166:Porphyrins / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship
D002735:Chlorophyllides; D011166:Porphyrins; C018395:tetracarboxyphenylporphine; C062985:phytochlorin
Issledovanie raspredelenie khlorina e6 i ego proizvodnykh v kletkakh HeLa metodom liuminestsentnoĭ mikroskopii.