Feasibility of using [18O2]11-dehydrothromboxane B2 as an internal standard of immunoaffinity purification followed by gas chromatography/selected ion monitoring.
[1,1-18O2]11-Dehydrothromboxane B2 (11-dehydro-TXB2) was prepared by repetitive base-catalyzed hydrolysis of the lactone ring of its [1,1,11-18O3]-analogue, and evaluated for its suitability as an internal standard in gas chromatography/selected ion monitoring (GC/SIM) of 11-dehydro-TXB2. Use of the present [18O2]-analogue as an internal standard may make it a suitable candidate for specific immunoaffinity purification followed by GC/SIM.
Ishibashi
M
M
Ohyama
Y
Y
Watanabe
K
K
Hayashi
Y
Y
Takasaki
W
W
Nakagawa
A
A
Mizugaki
M
M
D000328:Adult; D002846:Chromatography, Affinity; D002849:Chromatography, Gas; D006801:Humans; D007120:Immunochemistry; D008297:Male; D010104:Oxygen Radioisotopes; D013929:Thromboxane B2 / Q000031:analogs & derivatives* / Q000032:analysis
D010104:Oxygen Radioisotopes; D013929:Thromboxane B2; C049235:11-dehydro-thromboxane B2