Cellular activities associated with the transition of chick embryo fibroblasts from stationary to proliferation state.
Chick embryo fibroblasts on the 5th day of culture in proteinfree medium were stimulated to accelerated growth by supplementation of the medium with ATP (50 mumol/l, insulin (0.16 I.U./ml) or chick serum (5% v/v). Kinetics of the entry of cells into the S phase and later into the logarythmic phase of growth were found to be different in cultures treated with these three factors in spite that the final saturation densities reached after 30 days of culture were similar. No direct correlation between cell spreading and the cell transition from the stationary to the proliferation state was found. The proliferating cells showed the higher rate of locomotion than in stationary cultures. The initial protein-free medium, supporting the long survival of chick embryo fibroblasts and their susceptibility to growth accelerating factors, was further simplified by replacement of ADA buffer with EDTA (0.4 mmol/l).
Pietrzkowski
Z
Z
Department of Cell Biology, Jan Zurzycki Institute of Molecular Biology, Jagellonian University, Kraków, Poland.
Kajstura
J
J
Korohoda
W
W
D000818:Animals; D002455:Cell Division; D002642:Chick Embryo; D005347:Fibroblasts / Q000166:cytology*