title: [Asthma caused by Lucilia Caesar larvae: clinical and immunologic study].
abstract: Lucilia Caesar larvae (LCL) are used as live fish bait by anglers. Five cases of asthma and rhinoconjunctivitis following exposure to LCL are reported. Three had work-related asthma as they were working on a fish bait farm or shop and two had asthma when they went fishing. In one subject exposure to LCL caused asthma, rhinoconjunctivitis and contact urticaria. In four subjects peak expiratory flow rate (PEFR) was monitored during exposure to LCL. In three out of four subjects there was evidence of LCL-related asthma. In one subject it was not possible to record PEFR during exposure to LCL, as he had not gone fishing since 1985. Two extracts of LCL were prepared: one was the PBS (phosphate-buffered saline) washing fluid of LCL, the other was the PBS extract of homogenized LCL. Positive cutaneous prick tests to both LCL extracts were detected in three out of four symptomatic subjects. Specific IgE against both LCL extract antigens were found by the RAST method in four out of five subjects with LCL-related asthma. One subject had both negative skin tests and RAST. Specificity and potency of LCL-IgE binding was shown by RAST inhibition method performed on the serum pool of four patients with positive RAST results. Significant inhibition of more than 50% by LCL washing fluid at a dilution extract was found at a dilution of 1:10 and by homogenized LCL extract at a dilution of 1:100. No significant inhibition of LCL-IgE binding by dermatophagoides, parietaria and milk antigens was found. This study demonstrated that LCL emanations are potent sensitizers and elicit IgE-mediated asthma.
authors.forename: A
authors.initials: A
authors.lastname: Siracusa
authors.forename: A
authors.initials: A
authors.lastname: Verga
authors.forename: R
authors.initials: R
authors.lastname: Bacoccoli
authors.forename: A
authors.initials: A
authors.lastname: Fabbri
authors.forename: D
authors.initials: D
authors.lastname: Felicioni
mesh_terms: D000328:Adult; D000818:Animals; D001249:Asthma / Q000209:etiology* / Q000503:physiopathology; D006801:Humans; D007814:Larva; D008297:Male; D008875:Middle Aged; D009117:Muscidae; D009784:Occupational Diseases / Q000209:etiology* / Q000503:physiopathology; D010366:Peak Expiratory Flow Rate; D011852:Radioallergosorbent Test
vernacular_title: L'asma da bigattini (larve della mosca carnaria): studio clinico e immunologico.