The emergence and maturation of the first apatite crystals in an in vitro bone formation system.
Using chick periosteal bone forming cultures we have followed the appearance of the first crystals during de Novo bone formation. The first detectable mineral deposits are crystalline. By x-ray diffraction and dark field imaging we found crystal length to grow from around 100 angstroms on day 4 to (135-160) Angstroms by day 8. The Ca/P ratio rose from 1.46 at day 4 to 1.60 at day 8.
Grynpas
M D
MD
Department of Pathology, University of Toronto.
Tenenbaum
H C
HC
Holmyard
D P
DP
D000818:Animals; D001031:Apatites / Q000378:metabolism*; D001846:Bone Development / Q000502:physiology*; D002642:Chick Embryo; D003460:Crystallization; D046508:Culture Techniques; D014961:X-Ray Diffraction
D001031:Apatites