Protoplast transformation of Bacillus licheniformis MC14.
A protoplast transformation system has been developed for Bacillus licheniformis MC14. Optimum regeneration conditions were achieved by raising the incubation temperature of the regeneration plates to 46 degrees C. Regenerated transformed colonies could be isolated in 3 to 5 d under these conditions. Plasmids introduced by this method were stably maintained by B. licheniformis MC14 and could be recovered and used to transform Bacillus subtilis.
Jensen
K K
KK
Laboratory for Cell, Molecular, and Developmental Biology, University of Illinois, Chicago 60680.
Hulett
F M
FM
D001407:Bacillus / Q000235:genetics* / Q000502:physiology; D004269:DNA, Bacterial / Q000032:analysis; D010957:Plasmids; D011523:Protoplasts / Q000502:physiology; D012038:Regeneration; D014169:Transformation, Bacterial
D004269:DNA, Bacterial