Heterogeneity of ribosomal autoantibodies from human, murine and canine connective tissue diseases.
Antiribosomal auto-antibodies (anti-Rib.Ab) have been studied in connective tissue diseases (human, dog and mouse) by immunoblotting after one-dimensional (1D) or two-dimensional (2D) gel electrophoresis of rat ribosomes. Anti-Rib.Ab could be found in systemic lupus erythematosus (SLE), rheumatoid arthritis (RA) and other connective tissue diseases (progressive systemic sclerosis, PSS; Sjögren syndrome, SjS; mixed connective tissue disease, MCTD; and dermatomyositis, DM with the frequencies 41.7%, 54.6% and 33%, respectively. Immunoblotting after 1D gel electrophoresis showed the great heterogeneity of ribosomal proteins recognized by the anti-Rib.Ab. In the SLE, however, the most frequent antibodies stained bands of the 40S subunit: 30 kDa (34% of positive sera), 19.5 kDa (24.5%) and 43 kDa (17%). In RA, the 25-kDa band of the 60S subunit was the most common (54% of positive sera). In the other human connective tissue diseases, there was no particular predominance. In the MRL/1, anti-Rib.Ab were very frequent (92.6%). The 43-kDa band of the 40S subunit was found in 100% of positive sera. Seventeen out of nineteen dogs with SLE gave positive results on immunoblot, and all of them stained the 43-kDa band of the 40S subunit. 2D gel electrophoresis gave identification of Po, L7, L5, Sb, S19, S13 and L2 proteins in SLE, S3 and SjS, L35a and L37a in RA, and L7, S6 and/or L7a in MRL/1.
Absi
M
M
Laboratoire d'Immunologie, U.F.R. Alexis Carrel, Lyon, France.
La Vergne
J P
JP
Marzouki
A
A
Giraud
F
F
Rigal
D
D
Reboud
A M
AM
Reboud
J P
JP
Monier
J C
JC
D000818:Animals; D001172:Arthritis, Rheumatoid / Q000276:immunology; D001323:Autoantibodies / Q000302:isolation & purification*; D003240:Connective Tissue Diseases / Q000276:immunology*; D004285:Dogs; D006801:Humans; D015151:Immunoblotting; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D051379:Mice; D008970:Molecular Weight; D012269:Ribosomal Proteins / Q000276:immunology / Q000302:isolation & purification; D012270:Ribosomes / Q000276:immunology*
D001323:Autoantibodies; D012269:Ribosomal Proteins