Gangliosides in human urothelial cell lines of different transformed phenotypes: the effect of v-raf-oncogene transfection.
In a previous study we have shown that established human urothelial cell lines, representing grade of transformation II (TGr II, non-tumorigenic, non-invasive cells), are characterized by accumulation of the GM2 ganglioside as compared to cell lines of TGr III with tumorigenic and invasive properties. In the present study, the analysis of gangliosides from two tumorigenic sublines obtained after transfection of the TGr II cell line HCV 29 with the v-raf-oncogene, provided further evidence for the inverse relationship between tumorigenicity and the GM2 ganglioside expression. The two transfected sublines: T112C1 and T112D1, representing TGr III, were characterized by a decreased level of GM2 which was accompanied by an increased content of the GM3 ganglioside as compared to the parental HCV 29 cell line.
Ugorski
M
M
Department of Immunochemistry, Polish Academy of Sciences, Wroclaw.
Dus
D
D
Skouv
J
J
Påhlsson
P
P
Radzikowski
C
C
D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002851:Chromatography, High Pressure Liquid; D004848:Epithelium / Q000032:analysis; D005732:Gangliosides / Q000032:analysis*; D006801:Humans; D027384:Oncogene Proteins v-raf; D009857:Oncogenes; D010641:Phenotype; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics*; D014162:Transfection; D001743:Urinary Bladder / Q000166:cytology
D005732:Gangliosides; D015682:Retroviridae Proteins, Oncogenic; D011505:Protein-Tyrosine Kinases; D027384:Oncogene Proteins v-raf