Delay of behavioral estrus in hamsters and phenobarbital.
The onset of behavioral estrus was used as a phase marker of the hamster timing system in SLD 16:8 (dark 20:00-04:00). TZ was injected between 11:00 of cycle day 3 and noon of cycle day 4 when onset of estrus was determined. At no time did injection of TZ cause a phase advance in SLD 16:8. Small delays of estrus resulted from 11:00-16:00 injections but marked delays began with the 17:00 injection. Phenobarbital was injected between noon and 19:30 on cycle day 3. Injections between noon and 16:00 had no effect but all later injections beginning at 17:00 delayed estrus, the 17:30 injection causing the greatest delay. Diazepam also markedly delayed estrus when tested at 17:30. These results with three drugs support results with light pulses that 18:00 in SLD 16:8 marks the same phase of the 24-h hamster timing system as the onset of wheel running does in DD, LL, and WLD. These findings with three GABA potentiators extend to SLD previous evidence based on the onset of wheel running in DD, LL and WLD that GABA may be involved in hamster timekeeping and its responses to light and drugs.
Alleva
J J
JJ
Division of Research and Testing, Food and Drug Administration, Washington, D.C.
D000818:Animals; D002940:Circadian Rhythm / Q000187:drug effects; D006224:Cricetinae; D003624:Darkness; D003975:Diazepam / Q000494:pharmacology*; D004971:Estrus / Q000187:drug effects*; D005260:Female; D008027:Light; D008647:Mesocricetus; D009043:Motor Activity / Q000187:drug effects*; D010634:Phenobarbital / Q000494:pharmacology*; D014229:Triazolam / Q000494:pharmacology*; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
D014229:Triazolam; D005680:gamma-Aminobutyric Acid; D003975:Diazepam; D010634:Phenobarbital