[The oxygen supply of the isolated rat heart during perfusion with a suspension of human erythrocytes prepared on the blood preservative citroglucophosphate].
The isolated rat heart with the functioning left ventricle was used to measure the main parameters of hemodynamics and oxygen supply (60 experiments). Perfusion of the coronary vessels of the heart was performed with washed human red blood cells preserved for varying times (for 1, 7, 10, 14 and 21 days), stabilized on citroglucophosphate. The authors succeeded for the first time in demonstrating under artificially made conditions where the sole variable responsible for oxygen supply of the isolated organ was hemoglobin affinity for oxygen that there was no difference in the function of the isolated heart perfused with human red blood cells preserved for 7, 10 and 14 days as compared with freshly obtained ones. During perfusion of the heart with red blood cells preserved for an insignificant period of time (for 7 and 10 days), no physiological signs evidencing the impairment of oxygen supply to myocardial tissues were revealed.
Iarochkin
V S
VS
Vinogradov
S M
SM
Koziner
V B
VB
Agranenko
V A
VA
D000818:Animals; D001793:Blood Preservation; D002951:Citrates / Q000627:therapeutic use*; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D005947:Glucose / Q000627:therapeutic use*; D006801:Humans; D066298:In Vitro Techniques; D009206:Myocardium / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects* / Q000502:physiology; D010477:Perfusion / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains; D013535:Suspensions; D013997:Time Factors
D002951:Citrates; D013535:Suspensions; C007077:citrate phosphate dextrose; D005947:Glucose
Kislorodnoe snabzhenie izolirovannogo serdtsa krysy pri perfuzii ego vzves'iu éritrotsitov cheloveka, zagotovlennykh na gemokonservante tsitogliukofosfat.