title: Expression of MHC class I and class II antigens in colonic carcinomas.
abstract: Malignant and non-malignant ('normal') colonic tissues from patients with colonic carcinoma were examined for the expression of MHC class I and class II antigens by immunoenzymatic staining using monoclonal antibodies. The amount of class I antigen as detected by 2 monoclonal antibodies, FMC 16 or W6/32 was clearly diminished in 11 of 14 tumours when compared to the amount present on 'normal' colonic tissue from the same individual. The loss of class I antigen did not correlate with tumour stage or differentiation. The reactivities of FMC 16 and W6/32 with these tissues were not identical, which indicates that the 2 monoclonal antibodies may recognize different epitopes on the HLA class I molecule. Class II antigens were absent from 'normal' colonic epithelium but were present on 20 of 28 tumours, with DR being detected more often than DP, and DQ found only on 4 of 28 tumours. When present, staining for class II antigens was heterogeneous within the tumour, in that all tumour cells did not stain equally. DR and DP antigens were found more often on moderately or poorly differentiated adenocarcinomas and on stage B, C and D tumours in that order of frequency. Thus tumours with a better prognosis were less likely to express DR and DP. The expression of DQ was unrelated to staging or differentiation.
authors.affiliation: Department of Clinical Immunology, Flinders Medical Centre, Bedford Park, South Australia.
authors.forename: M
authors.initials: M
authors.lastname: Norazmi
authors.forename: A W
authors.initials: AW
authors.lastname: Hohmann
authors.forename: J M
authors.initials: JM
authors.lastname: Skinner
authors.forename: J
authors.initials: J
authors.lastname: Bradley
mesh_terms: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000276:immunology*; D002277:Carcinoma / Q000276:immunology*; D003106:Colon / Q000276:immunology; D003110:Colonic Neoplasms / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006801:Humans; D007413:Intestinal Mucosa / Q000276:immunology; D012016:Reference Values
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II