The promoter structure and complete sequence of the gene encoding the rabbit erythroid cell-specific 15-lipoxygenase.
We report the isolation and complete sequence of the gene encoding the rabbit erythroid-cell-specific 15-lipoxygenase (RBC 15-LOX), containing 14 exons spanning 8.0 kb. The transcription start point was mapped by S1 nuclease-protection experiments and comparison with the sequence of the RBC 15-LOX mRNA, as defined previously by primer extension experiments. The promoter contains a TATA-like motif, but no CCAAT motif in the canonical position, and lies within a 'CpG-rich island'. Functional analysis of the immediate 5'-flanking DNA by transfection experiments shows that a 150 nucleotide (nt) 5' fragment linked to the chloramphenicol acetyltransferase gene acts as a functional promoter in both erythroid and nonerythroid cell lines and responds in an erythroid-specific manner to the enhancer from the Friend murine leukaemia virus long terminal repeat, whereas a 40-nt fragment is inactive. Intron 7 contains eight copies of a 54-nt repeat containing a region with homology to the simian virus 40/immunoglobulin gene enhancers.
O'Prey
J
J
Beatson Institute for Cancer Research, Glasgow, U.K.
Chester
J
J
Thiele
B J
BJ
Janetzki
S
S
Prehn
S
S
Fleming
J
J
Harrison
P R
PR
D000818:Animals; D001093:Arachidonate 15-Lipoxygenase / Q000235:genetics*; D001091:Arachidonate Lipoxygenases / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D015226:Dinucleoside Phosphates / Q000032:analysis; D004742:Enhancer Elements, Genetic; D004912:Erythrocytes / Q000201:enzymology*; D005091:Exons; D015971:Gene Expression Regulation, Enzymologic; D005796:Genes; D007438:Introns; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011817:Rabbits; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014158:Transcription, Genetic
D001093:Arachidonate 15-Lipoxygenase; D001091:Arachidonate Lipoxygenases; D015226:Dinucleoside Phosphates; D015719:Single-Strand Specific DNA and RNA Endonucleases; C015772:CpG dinucleotide