[Macrophage activation by synthetic peptides. I. Kinetic changes in the transport of fluorescein anions across the plasma membrane of macrophages].
As shown by cytofluorimetric technique, fluorescein anions formed in macrophages due to hydrolysis of fluorescein diacetate (FDA) release into the extracellular medium through the probenecid-inhibitable transport system of organic acids. Technical procedures have been elaborated to record separately the process of FDA hydrolysis characterising the activity of intracellular esterases, and the fluorescein anion transport representing secretion of organic acids by macrophages. It has been established that the tetrapeptide tuftsin stimulates the cell esterase activity without affecting the rate of fluorescein efflux. The peptide KPR (Lys-Pro-Arg) decreases both the esterase activity and the fluorescein anion efflux.
Gamaleĭ
Ia
Ia
Kaulin
A B
AB
Kirpichnikova
K M
KM
D000595:Amino Acid Sequence; D000818:Animals; D000838:Anions; D001693:Biological Transport, Active / Q000187:drug effects / Q000502:physiology; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D005452:Fluoresceins / Q000493:pharmacokinetics*; D006868:Hydrolysis; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects* / Q000201:enzymology; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009283:Naphthol AS D Esterase / Q000378:metabolism; D009842:Oligopeptides / Q000494:pharmacology*; D010455:Peptides / Q000494:pharmacology*; D011339:Probenecid / Q000494:pharmacology; D014405:Tuftsin / Q000031:analogs & derivatives* / Q000494:pharmacology*
D000838:Anions; D005452:Fluoresceins; D009842:Oligopeptides; D010455:Peptides; C040816:tuftsin, Lys-Pro-Arg-; C060784:seryl-lysyl-aspartic acid; C062986:threonyl-lysyl-glutamic acid; C045468:threonyl-lysyl-proline; D009283:Naphthol AS D Esterase; D011339:Probenecid; D014405:Tuftsin
Aktivatsiia makrofagov sinteticheskimi peptidami. I. Izmenenie kinetiki transporta anionov fluorestseina cherez plazmaticheskuiu membranu makrofagov.