Linkage of PGK1 to X-linked severe combined immunodeficiency (IMD4) allows predictive testing in families with no surviving male.
We present a linkage map of DNA probes around the X-linked severe combined immunodeficiency (IMD4) locus at Xq11-13. DXS159 and PGK1 show no cross-overs with the disease locus (Lod 3.01 at theta = 0.00). The order of loci is DXS1-DXS106-(DXS159-PGK1-IMD4)-DXS72 -DXYS1. Members of families whose carrier status has been established by X-inactivation patterns were included in the analysis. As the probe (pSPT/PGK), which is used for investigation of X-inactivation patterns, has been shown to be linked to the disease itself, it is possible to assign phase in mothers of sporadic cases who have been shown to be carriers, even when they have no surviving male offspring.
Goodship
J
J
Department of Paediatric Genetics, Institute of Child Health, London, UK.
Levinsky
R
R
Malcolm
S
S
D015139:Blotting, Southern; D015342:DNA Probes; D005260:Female; D006580:Genetic Carrier Screening; D008040:Genetic Linkage; D005819:Genetic Markers / Q000032:analysis; D006239:Haplotypes; D006801:Humans; D007153:Immunologic Deficiency Syndromes / Q000175:diagnosis / Q000235:genetics*; D008126:Lod Score; D008297:Male; D010375:Pedigree; D010735:Phosphoglycerate Kinase / Q000235:genetics*; D014960:X Chromosome
D015342:DNA Probes; D005819:Genetic Markers; D010735:Phosphoglycerate Kinase