Importance of physical association between antigen and liposomes in liposomes adjuvanticity.
The importance of a physical association between antigen and liposomes in liposomes adjuvanticity has been evaluated by comparing the anti-BSA secondary humoral response induced by different BSA formulations including free antigen, free antigen + liposomes, or encapsulated antigen. Our results demonstrate that a physical association of antigen with liposomes is required for a potentiation of the humoral response to be observed and that empty liposomes, when used for priming together with free antigen, restrain the induced response, as shown by a slower triggering, a reduced intensity and a more rapid decay rate. Free BSA has an intermediate behavior and, in contrast to what is observed with BSA/liposomes preparations, its effect appears to be dose-dependent. Each of the three BSA formulations also differs in the relative production of IgG and IgM they induced during the response.
Thérien
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HM
Départment de Chimie-Biologie, Université du Québec à Trois-Rivières, Canada.
Shahum
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D000276:Adjuvants, Immunologic; D000818:Animals; D000917:Antibody Formation; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D007114:Immunization; D007700:Kinetics; D008081:Liposomes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D012710:Serum Albumin, Bovine / Q000276:immunology*
D000276:Adjuvants, Immunologic; D008081:Liposomes; D012710:Serum Albumin, Bovine