title: Complete sequence of the lamprey fibrinogen alpha chain.
abstract: The complete amino acid sequence of the lamprey fibrinogen alpha chain has been determined by a combination of peptide sequencing and cDNA and genomic cloning. The chain, which has an apparent molecular weight by dodecyl sulfate-polyacrylamide gel electrophoresis of ca. 100,000, is composed of 961 amino acid residues and has a calculated molecular weight of 96,722. It is distinguished by a large number of 18-residue repeats in a region where mammalian fibrinogens have 13-residue repeats. The data are in accord with our previous finding that the lamprey alpha chain has a distinctive amino acid composition, almost half the residues being glycine, serine, or threonine. The chain differs from mammalian alpha chains in that there are no cysteines in the carboxy-terminal half, and thus no intrachain loop, nor are there any RGD sequences in the lamprey alpha chain. Taken together with previous data on the sequences of the beta and gamma chains, the findings bear significantly on our understanding of fibrin formation. The alpha chain also provides an interesting case of structural convergence during evolution.
authors.affiliation: Center for Molecular Genetics, University of California, San Diego, La Jolla 92093.
authors.forename: Y Z
authors.initials: YZ
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: Patterson
authors.forename: J E
authors.initials: JE
authors.lastname: Gray
authors.forename: C
authors.initials: C
authors.lastname: Yu
authors.forename: B A
authors.initials: BA
authors.lastname: Cottrell
authors.forename: A
authors.initials: A
authors.lastname: Shimizu
authors.forename: D
authors.initials: D
authors.lastname: Graham
authors.forename: M
authors.initials: M
authors.lastname: Riley
authors.forename: R F
authors.initials: RF
authors.lastname: Doolittle
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003545:Cysteine / Q000235:genetics; D004247:DNA / Q000235:genetics; D005340:Fibrinogen / Q000235:genetics*; D005399:Fishes / Q000235:genetics*; D015698:Genomic Library; D007798:Lampreys / Q000235:genetics*; D008969:Molecular Sequence Data; D011487:Protein Conformation; D012091:Repetitive Sequences, Nucleic Acid; D013329:Structure-Activity Relationship
chemical_list: D005340:Fibrinogen; D004247:DNA; D003545:Cysteine