title: Rate of CO uptake by canine erythrocytes as a function of PO2.
abstract: We used a continuous-flow rapid-mixing apparatus with spectroscopic analysis to measure the rate of CO uptake by canine erythrocytes at 37 degrees C at five different PO2 values from 0 to 553 Torr. Fresh blood from five different dogs was used for the experiments. PCO approximated 80 Torr. Corrections for the lower capillary PCO during a measurement of the diffusing capacity of lung CO, as made by Roughton and Forster in 1957 (J. Appl. Physiol. 11: 290-302, 1957), were not used. The regression equation for 1/theta, where theta is milliliters of CO combining for each milliliter of whole blood (capacity 0.2 ml/ml) per minute for a PCO of 1 Torr was 1/theta = 1.45 +/- 0.0042 PO2. This equation is very similar to that for human erythrocytes under the same conditions.
authors.affiliation: Department of Physiology, School of Medicine, University of Pennsylvania, Philadelphia 19104.
authors.forename: R O
authors.initials: RO
authors.lastname: Crapo
authors.forename: N
authors.initials: N
authors.lastname: Bitterman
authors.forename: S L
authors.initials: SL
authors.lastname: Berlin
authors.forename: R E
authors.initials: RE
authors.lastname: Forster
mesh_terms: D000818:Animals; D002248:Carbon Monoxide / Q000378:metabolism*; D004285:Dogs; D004912:Erythrocytes / Q000378:metabolism*; D066298:In Vitro Techniques; D010100:Oxygen / Q000378:metabolism*
chemical_list: D002248:Carbon Monoxide; D010100:Oxygen