Characterization of a methyl-specific restriction system in Clostridium acetobutylicum strain N1-4081.
A type II restriction endonuclease, named CacI, was detected in Clostridium acetobutylicum strain N1-4081. CacI cleaved the tetranucleotide sequence [5' decreases -GATC-3']. The modification system consisted of the methylation of the adenine present in this sequence. CacI, an isoschizomer of MboI, is inactive on dam methylated substrates.
Azeddoug
H
H
Unité des Anaérobies, Institut Pasteur, Paris, France.
Hubert
J
J
Reysset
G
G
D000225:Adenine / Q000378:metabolism*; D001483:Base Sequence; D003013:Clostridium / Q000201:enzymology*; D004269:DNA, Bacterial / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D008745:Methylation; D010957:Plasmids
D004269:DNA, Bacterial; D015252:Deoxyribonucleases, Type II Site-Specific; C024405:GATC-specific type II deoxyribonucleases; D000225:Adenine