title: Presence of endopeptidase 24.11 during the ontogeny of the small intestine in different species.
abstract: The presence of endopeptidase 24.11 has been demonstrated at different periods of development in the small intestine of rat, rabbit and human, by immunoblotting using monoclonal antibodies raised against the rabbit kidney enzyme. The kinetic characteristics of the rabbit intestinal enzyme were determined after its purification by immunoaffinity column using the same antibodies. The endopeptidase 24.11 appears in the suckling rabbit as a low-affinity, high-capacity form, suggesting a role of this enzyme in the extracellular digestion of proteins in the young animal.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, University of Montreal, Canada.
authors.forename: H
authors.initials: H
authors.lastname: Lecavalier
authors.forename: P
authors.initials: P
authors.lastname: Crine
authors.forename: C
authors.initials: C
authors.lastname: Malo
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D004586:Electrophoresis; D004743:Enkephalin, Leucine / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D007421:Intestine, Small / Q000201:enzymology* / Q000254:growth & development; D007668:Kidney / Q000378:metabolism; D015260:Neprilysin / Q000032:analysis / Q000502:physiology*; D011817:Rabbits; D051381:Rats
chemical_list: D000911:Antibodies, Monoclonal; D004743:Enkephalin, Leucine; D015260:Neprilysin