Metabolism of a thromboxane A2 antagonist in isolated rat hepatocytes.
Metabolism of a thromboxane A2 antagonist (+/-)-5-(Z)-7-(3-endo-phenylsulphonylaminobicyclo[2.2.1]hept-2- exoyl)- heptenoic acid (S-145) in isolated rat hepatocytes was examined by high-performance liquid chromatography and gas chromatography/mass spectrometry. As the hydrophobic metabolites, bis-nor S-145, tetra-nor S-145 and bis-nordihydro S-145 were identified, and two other products were estimated to be monohydroxylated S-145 and dehydrogenated S-145. On the basis of kinetics of S-145 metabolism, a metabolic pathway of S-145 in isolated hepatocytes is tentatively proposed.
Higaki
J
J
Shionogi Research Laboratories, Shionogi & Co. Ltd.
Tonda
K
K
Takahashi
S
S
Hirata
M
M
D000818:Animals; D001643:Bridged Bicyclo Compounds / Q000378:metabolism* / Q000494:pharmacology; D001952:Bridged-Ring Compounds / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005229:Fatty Acids, Monounsaturated / Q000378:metabolism* / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013928:Thromboxane A2 / Q000037:antagonists & inhibitors*
D001643:Bridged Bicyclo Compounds; D001952:Bridged-Ring Compounds; D005229:Fatty Acids, Monounsaturated; C056566:S 145; D013928:Thromboxane A2