Polyclonal increase of HTLV-I provirus-carrying lymphocytes in HTLV-I-carrier rabbits transplanted with Shope carcinoma cells.
Fifteen HTLV-I carrier rabbits of two inbred strains and their F1 hybrid were inoculated with Shope carcinoma cells. The peripheral blood leukocyte counts in all these rabbits increased to 2-4 times the preinoculation level with a concomitant increase in abnormal lymphocytes. Southern blotting analyses of peripheral blood leukocytes revealed the polyclonal integration of HTLV-I proviral genome in all these animals. However, lymphocytic infiltration without any apparent leukemic infiltration was observed in major organs of these animals; a similar but slight lymphocytic infiltration was also observed in non-carrier control animals inoculated with Shope carcinoma cells. These results are interpreted as indicating that the growth of squamous cell carcinoma in HTLV-I carrier rabbits induced the polyclonal expansion of provirus-carrying cells, which may correspond to the intermediate state between healthy carrier and smouldering ATL.
Ogawa
K
K
Department of Pathology, Faculty of Medicine, Kyoto University, Japan.
Kawanishi
M
M
Matsuda
S
S
Isono
T
T
Seto
A
A
D000818:Animals; D015139:Blotting, Southern; D002277:Carcinoma / Q000150:complications* / Q000382:microbiology; D004279:DNA, Viral / Q000032:analysis; D015490:HTLV-I Infections / Q000150:complications / Q000382:microbiology* / Q000473:pathology; D007668:Kidney / Q000473:pathology; D007958:Leukocyte Count; D008099:Liver / Q000473:pathology; D008168:Lung / Q000473:pathology; D008214:Lymphocytes / Q000382:microbiology*; D009368:Neoplasm Transplantation; D011533:Proviruses / Q000502:physiology*; D011817:Rabbits
D004279:DNA, Viral