Inhibition of neutrophil locomotion, natural killer cell cytotoxicity and lymphocyte transformation by 1-methyl-3-phenyl-1,2,4-triazinium-5-olate, a novel triazinium zwitterion.
We studied a novel triazinium zwitterion compound for its effects on neutrophil locomotion and deoxyglucose uptake, Natural Killer (NK) cell cytotoxicity and mitogen-induced lymphocyte transformation. The results show significant inhibition of neutrophil locomotion at concentrations of 10 micrograms/ml or greater; by contrast, there was no significant effect on neutrophil deoxyglucose uptake. Significant suppression of NK cell cytotoxicity occurred at similar concentrations in a dose-dependent fashion. Marked suppression of mitogen-induced lymphocyte transformation was also observed for all three mitogens used in the assays. This effect was dose-dependent, reversible by washing and still evident even when it was added 37 h after the initiation of cultures. These results suggest that 1-methyl-3-phenyl-1,2,4-triazinium-5-olate may have application as an anti-inflammatory and immunosuppressive agent.
Vuddhakul
V
V
Department of Child Health, University of Queensland, Mater Children's Hospital, South Brisbane, Australia.
Jacobsen
N W
NW
Rose
S E
SE
Seow
W K
WK
Thong
Y H
YH
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D001693:Biological Transport, Active / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D003847:Deoxyglucose / Q000493:pharmacokinetics; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology; D066298:In Vitro Techniques; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects*; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology; D014227:Triazines / Q000494:pharmacology*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D007166:Immunosuppressive Agents; D014227:Triazines; C057604:1-methyl-3-phenyl-1,2,4-triazin-5-one; D003847:Deoxyglucose