title: Histochemical localization of cysteine-rich proteins by tissue printing on nitrocellulose.
abstract: A rapid technique for the histochemical localization of cysteine-rich proteins in plant tissues was developed. It is based on the immediate transfer of proteins to nitrocellulose membranes when a fresh cut organ is pressed against the membrane surface. The print was labeled for cysteine-rich proteins by reduction and alkylation of cysteinyl residues with dansylated iodoacetamide [N-iodoacetyl-N'-(-5-sulfo-1-naphthyl)ethylenediamine]. The S-carboxymethylated proteins were visualized by their fluorescence when excited with 360 nm light.
authors.affiliation: Department of Biology, Washington University, St. Louis, Missouri 63130.
authors.forename: R F
authors.initials: RF
authors.lastname: Pont-Lezica
authors.forename: J E
authors.initials: JE
authors.lastname: Varner
mesh_terms: D023181:Antimicrobial Cationic Peptides; D003101:Collodion; D003545:Cysteine / Q000032:analysis*; D006651:Histocytochemistry / Q000379:methods*; D001467:Hordeum / Q000032:analysis; D037102:Lectins / Q000032:analysis; D008567:Membranes, Artificial; D009282:Naphthalenesulfonates; D010084:Oxidation-Reduction; D037121:Plant Lectins; D010940:Plant Proteins / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D011198:Solanum tuberosum / Q000032:analysis
chemical_list: D023181:Antimicrobial Cationic Peptides; D037102:Lectins; D008567:Membranes, Artificial; D009282:Naphthalenesulfonates; D037121:Plant Lectins; D010940:Plant Proteins; D011506:Proteins; C006421:1,5-I-AEDANS; D003101:Collodion; C016085:purothionin; D003545:Cysteine