In situ methylation of human chromosomes with methylase-AluI.
A method for in situ DNA methylation with the prokaryotic methylase AluI has been developed for use on fixed human chromosomes. Incorporation of methyl groups into the chromosomal DNA has been shown by autoradiography using a labeled substrate. The methylation prevents the digestion of chromosomal DNA by AluI, allowing direct visualization of clusters of nonmethylated AGCT targets along the human complement.
Fernández-Piqueras
J
J
Departamento de Biología (Unidad de Genética), Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.
Ludeña
P
P
Sentís
C
C
D001483:Base Sequence; D001665:Binding Sites; D002871:Chromosome Banding; D002875:Chromosomes / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D015257:DNA-Cytosine Methylases / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008745:Methylation
D004247:DNA; C054926:DNA modification methylase AluI; D015257:DNA-Cytosine Methylases; C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific