High-performance liquid chromatographic procedure for the simultaneous determination of norfloxacin, fenbufen and felbinac in rat plasma.
A high-performance liquid chromatographic method for the simultaneous determination of norfloxacin, fenbufen and felbinac extracted from 50 microliters of rat plasma is described. Chromatography was performed on a reversed-phase column with ultraviolet detection. By the present method, quantitative and reproducible determinations were possible for norfloxacin, fenbufen and felbinac over the concentration ranges of 0.2-20, 0.2-120 and 0.4-40 micrograms/ml, respectively. The recoveries of norfloxacin, fenbufen and felbinac added to plasma were nearly 100% with a coefficient of variation of less than 8.0%. This method was found to be applicable to pharmacokinetic studies of each drug after the concomitant administration of norfloxacin and fenbufen.
Katagiri
Y
Y
Naora
K
K
Ichikawa
N
N
Hayashibara
M
M
Iwamoto
K
K
D000818:Animals; D008297:Male; D009643:Norfloxacin / Q000097:blood*; D010648:Phenylacetates / Q000097:blood*; D010654:Phenylbutyrates / Q000097:blood*; D051381:Rats; D011919:Rats, Inbred Strains
D010648:Phenylacetates; D010654:Phenylbutyrates; C012776:biphenylylacetic acid; C010725:fenbufen; D009643:Norfloxacin