title: Characterization of a methyl-specific restriction system in Clostridium acetobutylicum strain N1-4081.
abstract: A type II restriction endonuclease, named CacI, was detected in Clostridium acetobutylicum strain N1-4081. CacI cleaved the tetranucleotide sequence [5' decreases -GATC-3']. The modification system consisted of the methylation of the adenine present in this sequence. CacI, an isoschizomer of MboI, is inactive on dam methylated substrates.
authors.affiliation: Unité des Anaérobies, Institut Pasteur, Paris, France.
authors.forename: H
authors.initials: H
authors.lastname: Azeddoug
authors.forename: J
authors.initials: J
authors.lastname: Hubert
authors.forename: G
authors.initials: G
authors.lastname: Reysset
mesh_terms: D000225:Adenine / Q000378:metabolism*; D001483:Base Sequence; D003013:Clostridium / Q000201:enzymology*; D004269:DNA, Bacterial / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D008745:Methylation; D010957:Plasmids
chemical_list: D004269:DNA, Bacterial; D015252:Deoxyribonucleases, Type II Site-Specific; C024405:GATC-specific type II deoxyribonucleases; D000225:Adenine