Determination of glucose-6-phosphate dehydrogenase isoenzymes of human erythrocytes by a thin-layer PAG-IEF procedure.
A procedure of thin-layer PAG-IEF was employed to separate glucose-6-phosphate dehydrogenase (G6PD) isoenzymes of human erythrocytes into 15-16 bands in a pH range between 4.7 and 6.5. The G6PD isoenzymes were scanned with a dual-wavelength thin-layer chromatoscanner and the relative enzyme content of each band was calculated. The method possesses the merits of simplicity, high sensitivity, rapidity and high reproducibility.
Nie
C X
CX
Human University of Medical Science, Changsha, China.
Tong
S T
ST
Chen
D X
DX
D002855:Chromatography, Thin Layer / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004912:Erythrocytes / Q000201:enzymology*; D005954:Glucosephosphate Dehydrogenase / Q000097:blood* / Q000302:isolation & purification; D006801:Humans; D007527:Isoenzymes / Q000097:blood* / Q000302:isolation & purification
D007527:Isoenzymes; D005954:Glucosephosphate Dehydrogenase