The application of the maximum entropy method to electron microscopic tomography.
The maximum entropy method has been applied to single axis tilt electron microscopic tomography. Its application requires that the problem be correctly formulated and that the model for the noise in electron micrographs be developed. A suitable noise model was determined empirically. The maximum entropy method was applied to a reconstruction of a test object from projections to which noise had been added. These reconstructions were superior to those obtained by reciprocal space weighted back protection. The method was also robust towards the incorrect specification of the noise, the penalty being an increase in the time required for convergence rather than degradation of the quality of the reconstructed image. In the reconstruction of negatively stained chromatin fibres it was possible to obtain satisfactory images utilizing all the information in the projections, in contrast to conventional methods in which high resolution data are removed by the application of Fourier space filters.
Lawrence
M C
MC
Electron Microscopy Unit, University of Cape Town, Rondebosch, South Africa.
Jaffer
M A
MA
Sewell
B T
BT
D000465:Algorithms; D000818:Animals; D002843:Chromatin / Q000648:ultrastructure*; D003720:Densitometry; D007091:Image Processing, Computer-Assisted; D008297:Male; D008854:Microscopy, Electron / Q000379:methods*; D012044:Regression Analysis; D012617:Sea Urchins; D013094:Spermatozoa / Q000648:ultrastructure*; D014054:Tomography / Q000379:methods*
D002843:Chromatin