Analysis of the major urinary thromboxane metabolites, 2,3-dinorthromboxane B2 and 11-dehydrothromboxane B2, by gas chromatography-mass spectrometry and gas chromatography-tandem mass spectrometry.
2,3-Dinorthromboxane B2 and 11-dehydrothromboxane B2, the two major metabolites of thromboxane B2, are considered to be indices of thromboxane A2 activity in humans. The determination of these metabolites in urine was comparatively performed by gas chromatography-mass spectrometry and gas chromatography-mass spectrometry-mass spectrometry using the corresponding chemically synthesized tetradeuterated analogues as internal standards. The urine samples of five females and two males, all healthy, were prepurified by solid-phase extraction. The corresponding pentafluorobenzyl ester derivatives were repurified by high-performance liquid chromatography. The concentrations of 2,3-dinorthromboxane B2 and 11-dehydrothromboxane B2 ranged from 21 to 266 pg/ml and 47 to 942 pg/ml, respectively. The ratio of urinary 2,3-dinorthromboxane B2 to 11-dehydrothromboxane B2 varied from 1:3 to 1:5, except for one sample with nearly equal concentrations of 2,3-dinorthromboxane B2 and 11-dehydrothromboxane B2.
Uedelhoven
W M
WM
Institut für Prophylaxe und Epidemiologie der Kreislauferkrankungen Universität München, F.R.G.
Meese
C O
CO
Weber
P C
PC
D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D013929:Thromboxane B2 / Q000031:analogs & derivatives* / Q000652:urine; D013931:Thromboxanes / Q000378:metabolism*
D013931:Thromboxanes; D013929:Thromboxane B2; C028965:2,3-dinor-thromboxane B2; C049235:11-dehydro-thromboxane B2