title: Feasibility of using [18O2]11-dehydrothromboxane B2 as an internal standard of immunoaffinity purification followed by gas chromatography/selected ion monitoring.
abstract: [1,1-18O2]11-Dehydrothromboxane B2 (11-dehydro-TXB2) was prepared by repetitive base-catalyzed hydrolysis of the lactone ring of its [1,1,11-18O3]-analogue, and evaluated for its suitability as an internal standard in gas chromatography/selected ion monitoring (GC/SIM) of 11-dehydro-TXB2. Use of the present [18O2]-analogue as an internal standard may make it a suitable candidate for specific immunoaffinity purification followed by GC/SIM.
authors.forename: M
authors.initials: M
authors.lastname: Ishibashi
authors.forename: Y
authors.initials: Y
authors.lastname: Ohyama
authors.forename: K
authors.initials: K
authors.lastname: Watanabe
authors.forename: Y
authors.initials: Y
authors.lastname: Hayashi
authors.forename: W
authors.initials: W
authors.lastname: Takasaki
authors.forename: A
authors.initials: A
authors.lastname: Nakagawa
authors.forename: M
authors.initials: M
authors.lastname: Mizugaki
mesh_terms: D000328:Adult; D002846:Chromatography, Affinity; D002849:Chromatography, Gas; D006801:Humans; D007120:Immunochemistry; D008297:Male; D010104:Oxygen Radioisotopes; D013929:Thromboxane B2 / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: D010104:Oxygen Radioisotopes; D013929:Thromboxane B2; C049235:11-dehydro-thromboxane B2