Effect of interleukin-6/interferon-beta 2 on glucocorticoid action in rat hepatoma cells.
Reuber hepatoma cells (RHC) were treated 4 h with dexamethasone (dex), with and without simultaneous fibroblast-conditioned medium (cIL-6). A cytosol fraction, prepared in the presence of molybdate and dithiothreitol, was analyzed for [3H]dex (20 nM) binding in the presence and absence of 1 microM dex at 4 degrees C. Receptor levels declined from 76.0 fmol/mg at zero dex to 28.8 fmol/mg at 10 nM dex, and to 11.8 fmol/mg at 1 microM dex (P less than or equal to 0.05). cIL-6 plus 10 nM dex lowered binding to 18.3 fmol/mg (P less than or equal to 0.05), and treatment with cIL-6 alone diminished binding to 9.8 fmol/mg (P less than or equal to 0.05). Thus, cIL-6 diminished the number of available glucocorticoid receptors.
Stith
R D
RD
Department of Physiology and Biophysics, University of Oklahoma Health Sciences Center, Oklahoma City 73190.
McCallum
R E
RE
Hill
M R
MR
D000818:Animals; D002460:Cell Line; D003600:Cytosol / Q000378:metabolism; D003907:Dexamethasone / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D007375:Interleukin-1 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000187:drug effects / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology
D007370:Interferon Type I; D007375:Interleukin-1; D015850:Interleukin-6; D011965:Receptors, Glucocorticoid; D011994:Recombinant Proteins; C028697:dexamethasone receptor; D003907:Dexamethasone