title: Electrochemical detection of peptides.
abstract: A general method of wide applicability for the determination of peptides is described. Peptides longer than dipeptides react in the classical biuret reaction with Cu(II) to yield electroactive Cu(II)-peptide complexes that can be oxidized to the corresponding Cu(III) complexes. This allows the sensitive electrochemical detection of peptides following their separation by reversed-phase liquid chromatography. The reaction chemistry, which is reversible, allows for the determination of peptides that lack an electroactive group or a primary amine. Selectivity for a model peptide is 10(3)-10(4) over nonelectroactive amino acids.
authors.forename: A M
authors.initials: AM
authors.lastname: Warner
authors.forename: S G
authors.initials: SG
authors.lastname: Weber
mesh_terms: D004563:Electrochemistry; D010455:Peptides / Q000032:analysis*
chemical_list: D010455:Peptides