A class II patatin promoter is under developmental control in both transgenic potato and tobacco plants.
A new member of the patatin gene family belonging to the class II subfamily was isolated and characterized by DNA sequencing. In order to study the expression profile of this gene, the promoter was fused to the beta-glucuronidase gene and transferred to potato and tobacco. Histochemical analysis revealed high expression in a few defined cells in potato tubers and in a specific layer of both potato and tobacco root tips. In contrast to the developmentally and metabolically regulated class I patatin gene B33 this gene was not inducible by elevated levels of sucrose. Expression of this chimaeric gene was also found in callus and suspension cultures of potato.
Köster-Töpfer
M
M
Institut für Genbiologische Forschung Berlin GmbH, Federal Republic of Germany.
Frommer
W B
WB
Rocha-Sosa
M
M
Rosahl
S
S
Schell
J
J
Willmitzer
L
L
D001483:Base Sequence; D002265:Carboxylic Ester Hydrolases; D003001:Cloning, Molecular; D015870:Gene Expression; D017343:Genes, Plant; D005966:Glucuronidase / Q000235:genetics; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000096:biosynthesis / Q000145:classification / Q000235:genetics*; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D011198:Solanum tuberosum / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics*; D014170:Transformation, Genetic
D010940:Plant Proteins; C043222:patatin protein, Solanum tuberosum; D002265:Carboxylic Ester Hydrolases; D005966:Glucuronidase