No embryotoxicity of exogenous alpha-elastin evidenced through interference with the chick embryonic morphogenetic systems.
The effects of alpha-elastin (1) on the morphogenetic systems of the chick embryo were studied by means of the CHEST (chick embryotoxicity screening test). The subgerminal administration of doses of 0.3 mg and larger induced a growth retardation of the caudal morphogenetic systems (CMS). The administration of single effective doses of 1 subgerminally on the second day and intraamniotically on the third and fourth d of embryogenesis verified the beginning of the direct embryotoxicity range between the doses of 0.3 and 1 mg per embryo. It presents in the theoretical extrapolation for mammals the dose limits between 1 and 10 g.kg-1 of maternal weight. Embryotoxic manifestations (body wall defects, growth retardation and embryolethality) were induced predominantly in the embryos treated on d 2 with the very high doses only. Their frequency was rare. It has been concluded that 1 could be a safe potential natural drug carrier in clinical practice.
Kosar
K
K
Department of Pharmacological Propaedeutics, Faculty of Pharmacy, Charles University, Hradec Králové, Czechoslovakia.
Wimmerová
J
J
Bartos
F
F
D000818:Animals; D002642:Chick Embryo; D004549:Elastin / Q000633:toxicity*; D006128:Growth / Q000187:drug effects; D013723:Teratogens
D013723:Teratogens; D004549:Elastin