title: Application of polymerase chain reaction to detect activated oncogenes in hematological malignancies.
abstract: Polymerase chain reaction (PCR) was applied to detect the structural change accompanying the activation of oncogenes in hematological malignancies and preleukemic states. Point mutation of N-ras oncogene was examined by oligonucleotide differential hybridization coupled with PCR. Five out of 17 AML patients were shown to have mutated N-ras gene. These mutations could be used as a genetic marker to diagnose the residual malignant cells. Philadelphia chromosome in CML was examined by cDNA synthesis and PCR with successful results. PCR was shown to be a highly versatile and sensitive method which would be invaluable in clinical diagnosis.
authors.forename: F
authors.initials: F
authors.lastname: Ishikawa
authors.forename: Y
authors.initials: Y
authors.lastname: Kobayashi
mesh_terms: D001483:Base Sequence; D006801:Humans; D007938:Leukemia / Q000235:genetics*; D008969:Molecular Sequence Data; D009190:Myelodysplastic Syndromes / Q000235:genetics*; D009857:Oncogenes / Q000235:genetics*; D016133:Polymerase Chain Reaction