[Isolation and purification of collagen proteins using biospecific chromatography on a sorbent with collagen-binding fragment of fibronectin].
A new procedure is described for isolation of collagens from various preparations containing small amounts of these proteins. The procedure is based on specific affinity of collagens of various types to one of peptide fragments of fibronectin--collagen-binding peptide (CBP). Collagens were isolated after single-step chromatography of tissue homogenate on the sorbent containing immobilized CBP. High specificity and reproducibility of the procedure was shown when various preparations were used: chicken embryo proteins with total radioactive label. Proteins synthesized in fibroblast culture; polypeptides synthesized in cell-free system of protein synthesis.
Bychkova
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VV
Berman
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AE
D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity / Q000379:methods*; D003094:Collagen / Q000097:blood / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D005353:Fibronectins; D006801:Humans; D010446:Peptide Fragments
D005353:Fibronectins; D010446:Peptide Fragments; D003094:Collagen
Vydelenie i ochistka kollagenovykh belkov s pomoshch'iu biospetsificheskoĭ khromatografii na sorbente s kollagensviazyvaiushchim fragmentom fibronektina.