title: Assimilation of methylamine by Paracoccus denitrificans involves formaldehyde transport by a specific carrier.
abstract: Assimilation of methylamine by Paracoccus denitrificans involves the following enzymes: a periplasmic methylamine dehydrogenase, a formaldehyde transport system, cytoplasmic formaldehyde and formate dehydrogenase. Formaldehyde transport follows saturation kinetics with a high substrate affinity (Km = 7 microM), and is severely inhibited by iodoacetate, cyanide and p-trifluoromethoxy carbonylcyanide phenylhydrazone. Expression of the formaldehyde carrier is regulated by the carbon source.
authors.affiliation: Lehrstuhl für Mikrobiologie, Universität Bayreuth, F.R.G.
authors.forename: M
authors.initials: M
authors.lastname: Köstler
authors.forename: D
authors.initials: D
authors.lastname: Kleiner
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001692:Biological Transport; D005557:Formaldehyde / Q000378:metabolism*; D005560:Formate Dehydrogenases / Q000378:metabolism; D007700:Kinetics; D008744:Methylamines / Q000378:metabolism*; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000378:metabolism; D010231:Paracoccus denitrificans / Q000378:metabolism*
chemical_list: D008744:Methylamines; D005557:Formaldehyde; D000255:Adenosine Triphosphate; D005560:Formate Dehydrogenases; C021567:methylamine dehydrogenase; D000587:Oxidoreductases Acting on CH-NH Group Donors