title: Evaluation of serum iso-amylase in the normal dog using an improved electrophoretic technique.
abstract: Six iso-amylase fractions are known to exist in human serum, three originating from the salivary glands and three from the pancreas. Although it is known that a different number and source of iso-amylase fractions occur in the dog, the routine detection of all the canine iso-amylase fractions has not been previously established. Earlier methods detected either two iso-amylase fractions or, in a proportion of cases, four fractions. A method is described which detects four iso-amylase fractions in 95% of normal canine serum samples. Trials have shown the method is reproducible and that freezing at -40 degrees C has no effect on iso-amylase activity. The normal values for iso-amylase are recorded in 18 normal dogs.
authors.affiliation: Department of Veterinary Clinical Studies, Royal (Dick) School of Veterinary Studies, Edinburgh, UK.
authors.forename: J W
authors.initials: JW
authors.lastname: Simpson
authors.forename: D L
authors.initials: DL
authors.lastname: Doxey
authors.forename: G
authors.initials: G
authors.lastname: Keay
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D004285:Dogs / Q000097:blood*; D004586:Electrophoresis / Q000379:methods*; D004588:Electrophoresis, Cellulose Acetate / Q000379:methods*; D005069:Evaluation Studies as Topic; D005260:Female; D005615:Freezing; D006026:Glycoside Hydrolases / Q000097:blood*; D007517:Isoamylase / Q000097:blood*; D008297:Male; D012016:Reference Values; D015203:Reproducibility of Results; D013045:Species Specificity
chemical_list: D006026:Glycoside Hydrolases; D007517:Isoamylase