[Mechanisms of impairment of DNA repair in human cells. Effects of native and recombinant interferons in UV-irradiated human fibroblasts].
Effects of native and recombinant interferons in normal and xeroderma pigmentosum (XP) human fibroblasts were studied. The criteria to evaluate the effects of interferons were: inhibition of replicative DNA synthesis, modification of replicative and unscheduled DNA synthesis in UV-irradiated cells. It was demonstrated that the level of inhibition of replicative DNA synthesis with interferons was dependent on proliferative status of cell cultures. The level of 3H-thymidine incorporation after stimulation of cell proliferation was increased in UV-irradiated normal fibroblasts pretreated with interferons. Interferon effect was not observed in XP cells. In cell cultures with low level of proliferation treatment with interferon resulted in inhibition of DNA replication and pronounced increase in the unscheduled DNA synthesis. In quiescent cells the effect of interferon on unscheduled DNA synthesis in UV-irradiated cells was not pronounced. In XP cells UV-irradiation induced low unscheduled DNA synthesis which was not modified by interferon.
Sinel'shchikova
T A
TA
Gubitskaia
E G
EG
Zasukhina
G D
GD
D002478:Cells, Cultured; D004249:DNA Damage; D004260:DNA Repair; D004261:DNA Replication; D005347:Fibroblasts / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D011994:Recombinant Proteins; D014466:Ultraviolet Rays; D014983:Xeroderma Pigmentosum / Q000235:genetics
D007370:Interferon Type I; D011994:Recombinant Proteins
Mekhanizmy narusheniĭ reparatsii DNK v kletkakh cheloveka. Effekty nativnogo i rekombinantnogo interferonov v UF-obluchennykh fibroblastakh cheloveka.