title: Failure of N-(2,4-dinitrophenyl)-5-methoxytryptamine (a putative melatonin antagonist) and of N-(3,5-dinitrophenyl)-5-methoxytryptamine to prevent the effects of injections of melatonin in the late afternoon on testicular activity of the golden hamster.
abstract: Daily injections of 10 micrograms melatonin in the late afternoon into male golden hamsters kept under a long photoperiod (14 h light: 10 h darkness) and at low ambient temperature 6 +/- 1 degrees C) induced a complete gonadal atrophy after 4 weeks. When administered under the same conditions at doses of 25 micrograms, neither N-(3,5-dinitrophenyl)-5-methoxytryptamine or N-(2,4-dinitrophenyl)-5-methoxytryptamine, a putative melatonin antagonist termed ML-23 in the literature, showed any effect on testicular activity. Moreover, these two drugs were also unable to prevent melatonin-induced gonadal atrophy when injected 30 min before melatonin. The results demonstrate that in the golden hamster and in the present experimental conditions, these drugs do not have the melatonin-antagonistic properties as described in the rat.
authors.affiliation: URA-CNRS 1332 Neurobiologie des fonctions rythmiques et saisonnières, Laboratoire de Zoologie, Université Louis Pasteur, Strasbourg, France.
authors.forename: P
authors.initials: P
authors.lastname: Pévet
authors.forename: M
authors.initials: M
authors.lastname: Masson-Pévet
authors.forename: J
authors.initials: J
authors.lastname: Chantegrel
authors.forename: A
authors.initials: A
authors.lastname: Marsura
authors.forename: C
authors.initials: C
authors.lastname: Luu-Duc
authors.forename: B
authors.initials: B
authors.lastname: Claustrat
mesh_terms: D008735:5-Methoxytryptamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D001284:Atrophy / Q000139:chemically induced; D003080:Cold Temperature; D006224:Cricetinae; D008027:Light; D008297:Male; D008550:Melatonin / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D008647:Mesocricetus; D012701:Serotonin / Q000031:analogs & derivatives*; D013737:Testis / Q000187:drug effects* / Q000473:pathology
chemical_list: C053008:ML 23; C059440:N-(2,4-dinitrophenyl)-5-methoxytryptamine; D012701:Serotonin; D008735:5-Methoxytryptamine; D008550:Melatonin