Abnormalities of calcium ion movement in platelets of patients with myeloproliferative disorders.
We investigated abnormalities in calcium ion influx in platelets of patients with myeloproliferative disorders (MPD). 45Ca2+ influx into the cytosol of MPD platelets was lower than that of normal controls. To determine whether the low Ca2+ influx is caused by a functional abnormality of membrane glycoprotein (GP) IIb-IIIa complex for Ca2+ channel or not, we investigated the Ca2+ influx through GP IIb-IIIa complex, which was reconstituted into liposomes. The purified GP IIb-IIIa complex from platelets of 5 patients was reconstituted into phospholipid liposomes. Ca2+ influx into the liposomes measured by fluorescence of intravesicular Fura-2 was lower in 2 of these patients. These findings corresponded with the results of intracellular calcium concentration after stimulation in these platelets. We concluded that functional abnormalities of GP IIb-IIIa is involved in the mechanism of impaired Ca2+ influx in MPD platelets.
Fujimoto
T
T
Fujimura
K
K
Kuramoto
A
A
D001792:Blood Platelets / Q000378:metabolism*; D002118:Calcium / Q000097:blood*; D006801:Humans; D009196:Myeloproliferative Disorders / Q000097:blood*
D002118:Calcium