[Use of amphotericin B-cells to detect inhibitors of cellular cholesterol biosynthesis].
A new method for detecting inhibitors of cellular cholesterol biosynthesis is reported. It was established according to the principle that amphotericin B binds sterols in the membranes of animal cells to form a complex which kills the cells. The method was simple, reliable and easy to handle. Moreover, the cost was low. Using this method, the authors had screened twenty Chinese herbs and found that Salvia miltiorrhiza and its extracts inhibit cellular cholesterol biosynthesis efficiently.
Sun
X M
XM
Cai
H J
HJ
D000666:Amphotericin B; D000818:Animals; D000924:Anticholesteremic Agents; D002478:Cells, Cultured; D002784:Cholesterol / Q000096:biosynthesis*; D006224:Cricetinae; D004338:Drug Combinations / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D010618:Phenanthrolines / Q000494:pharmacology; D010936:Plant Extracts; D027561:Salvia miltiorrhiza
D000924:Anticholesteremic Agents; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D010618:Phenanthrolines; D010936:Plant Extracts; C028972:dan-shen root extract; D000666:Amphotericin B; D002784:Cholesterol