Immature cells identified by the H-1 hematology analyzer predicts thymidine incorporation after mitogenic stimulation.
The incorporation of thymidine by lymphocytes stimulated by phytohemagglutinin (PHA) was correlated with the number of immature cells identified by the Technicon H-1 as blasts. The number of blasts and thymidine incorporation plateaued on day 3. At that time, a high linear correlation between the two variables was found (r = 0.949, p less than 0.01). The H-1 evaluated the samples in 60 seconds as compared to 3 hours of processing required to calculate thymidine incorporation. We conclude that the number of immature cells identified by the Technicon H-1 can predict the degree of thymidine incorporation after stimulation of peripheral blood cells with PHA. The H-1 may replace the more time consuming and dangerous use of radioactive thymidine incorporation in evaluating patients' functional immunologic status.
Froom
P
P
Institute of Hematology, Lady Davis Carmel Hospital, Haifa, Israel.
Kinarty
A
A
Aghai
E
E
Lahat
N
N
D001772:Blood Cell Count; D006403:Hematologic Tests; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism; D010835:Phytohemagglutinins / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism
D010835:Phytohemagglutinins; D013936:Thymidine