Complete separation of anti-hapten antibodies by two-dimensional affinity electrophoresis.
A high resolution two-dimensional affinity electrophoresis has been developed, using capillary isoelectric focusing as the first electrophoresis and slab gel affinity electrophoresis as second electrophoresis. By this method 1-2 micrograms of anti-dinitrophenyl antibodies have been separated completely into several hundred homogeneous IgG spots. They are grouped into a number of families which are composed of several IgG spots of the same affinity to the hapten but of a different pI. It is suggested that each individual family is derived from one monoclonal antibody producing cell line.
Takeo
K
K
First Department of Biochemistry, Yamaguchi University School of Medicine, Ube, Japan.
Suzuno
R
R
Tanaka
T
T
Nakamura
K
K
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006241:Haptens / Q000276:immunology; D007074:Immunoglobulin G / Q000302:isolation & purification; D011817:Rabbits
D000906:Antibodies; D000911:Antibodies, Monoclonal; D006241:Haptens; D007074:Immunoglobulin G