Bacterial colony screening with a Streptomyces DNA probe.
Restriction fragments of 1.5 kb-3.5 kb length were selected from a SalI digest of Streptomyces coriofaciens ISP5485 DNA. After radiolabelling, these fragments were used as a molecular probe. A number of actinomycetes was screened in colony hybridization. Streptomyces and Streptoverticillium strains were recognized by the probe and the hybridization sensitivity was high with isolated DNA.
Kuczek
K
K
Polish Academy of Sciences, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Wroclaw.
Mordarski
M
M
D000192:Actinomycetales / Q000166:cytology / Q000235:genetics; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D013302:Streptomyces / Q000235:genetics*
D015342:DNA Probes; D004269:DNA, Bacterial