Determination of pentoxifylline and its 5-hydroxy metabolite in human plasma by solid-phase extraction and high-performance liquid chromatography with ultraviolet detection.
The simultaneous determination of pentoxifylline and its 5-hydroxy metabolite in human plasma was performed: 10 ng/ml pentoxifylline and 15 ng/ml metabolite were determined at a signal-to-noise ratio of 3. The recoveries from plasma at a 100 ng/ml level were 98.0 and 86.9% for pentoxifylline and the metabolite, respectively. The intra- and inter-assay coefficients of variation were less than 5% for both pentoxifylline and the metabolite. This method shows advantages over many other published extraction procedures prior to high-performance liquid chromatographic analysis in terms of its speed and ease of manipulation.
Musch
G
G
Vrije Universiteit Brussel, Farmaceutisch Instituut, Belgium.
Hamoir
T
T
Massart
D L
DL
D000328:Adult; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D008297:Male; D010431:Pentoxifylline / Q000097:blood* / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D013805:Theobromine / Q000031:analogs & derivatives*
D013805:Theobromine; D010431:Pentoxifylline