Three novel oligosaccharides with the sialyl-Lea structure in human milk: isolation by immunoaffinity chromatography.
We have determined the structures of three novel oligosaccharides isolated from human milk using the monoclonal antibody MSW 113. These oligosaccharides were purified by affinity chromatography on a column of the immobilized monoclonal antibody and by high-performance liquid chromatography. From the results of 500-MHz 1H NMR spectroscopy and fast atom bombardment-mass spectrometry, their structures were deduced to be (formula; see text) These oligosaccharides bound to MSW 113 to nearly the same extent as sialyl-Lea hexasaccharide but bound to another sialyl-Lea structure-directed monoclonal antibody, NS 19-9, only weakly.
Kitagawa
H
H
Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
Nakada
H
H
Kurosaka
A
A
Hiraiwa
N
N
Numata
Y
Y
Fukui
S
S
Funakoshi
I
I
Kawasaki
T
T
Yamashina
I
I
Shimada
I
I
D002240:Carbohydrate Sequence; D002846:Chromatography, Affinity / Q000379:methods; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008895:Milk, Human / Q000032:analysis*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis*; D014407:Tumor Cells, Cultured
D009844:Oligosaccharides; C016735:sialooligosaccharides