title: Inhibition of neutrophil locomotion, natural killer cell cytotoxicity and lymphocyte transformation by 1-methyl-3-phenyl-1,2,4-triazinium-5-olate, a novel triazinium zwitterion.
abstract: We studied a novel triazinium zwitterion compound for its effects on neutrophil locomotion and deoxyglucose uptake, Natural Killer (NK) cell cytotoxicity and mitogen-induced lymphocyte transformation. The results show significant inhibition of neutrophil locomotion at concentrations of 10 micrograms/ml or greater; by contrast, there was no significant effect on neutrophil deoxyglucose uptake. Significant suppression of NK cell cytotoxicity occurred at similar concentrations in a dose-dependent fashion. Marked suppression of mitogen-induced lymphocyte transformation was also observed for all three mitogens used in the assays. This effect was dose-dependent, reversible by washing and still evident even when it was added 37 h after the initiation of cultures. These results suggest that 1-methyl-3-phenyl-1,2,4-triazinium-5-olate may have application as an anti-inflammatory and immunosuppressive agent.
authors.affiliation: Department of Child Health, University of Queensland, Mater Children's Hospital, South Brisbane, Australia.
authors.forename: V
authors.initials: V
authors.lastname: Vuddhakul
authors.forename: N W
authors.initials: NW
authors.lastname: Jacobsen
authors.forename: S E
authors.initials: SE
authors.lastname: Rose
authors.forename: W K
authors.initials: WK
authors.lastname: Seow
authors.forename: Y H
authors.initials: YH
authors.lastname: Thong
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D001693:Biological Transport, Active / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D003847:Deoxyglucose / Q000493:pharmacokinetics; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology; D066298:In Vitro Techniques; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects*; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology; D014227:Triazines / Q000494:pharmacology*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D007166:Immunosuppressive Agents; D014227:Triazines; C057604:1-methyl-3-phenyl-1,2,4-triazin-5-one; D003847:Deoxyglucose