title: Molecular cloning, nucleotide sequence and fine-structural analysis of the Corynebacterium glutamicum fda gene: structural comparison of C. glutamicum fructose-1,6-biphosphate aldolase to class I and class II aldolases.
abstract: The Corynebacterium glutamicum fda gene encoding fructose-1,6-biphosphate (FBP) aldolase has been isolated by complementation of an Escherichia coli mutant. The nucleotide sequence of a 3371 bp chromosomal fragment containing the C. glutamicum fda gene was determined. The N-terminal amino acid sequence of C. glutamicum FBP aldolase identified the correct initiation site for the fda gene, and a molecular weight of 37,092 was predicted for the fda polypeptide. S1 nuclease mapping identified the transcriptional start site, and Northern hybridization analysis indicated that the fda gene encodes a single 1.3 kb transcript. The primary structure of C. glutamicum FBP aldolase shows strong homology to class II FBP aldolases. Conservation of primary structure was observed between class I and class II aldolases, but several residues essential for catalytic activity in class I aldolases were absent from class II aldolases.
authors.affiliation: Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
authors.forename: C H
authors.initials: CH
authors.lastname: von der Osten
authors.forename: C F
authors.initials: CF
authors.lastname: Barbas
authors.forename: C H
authors.initials: CH
authors.lastname: Wong
authors.forename: A J
authors.initials: AJ
authors.lastname: Sinskey
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003352:Corynebacterium / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D005634:Fructose-Bisphosphate Aldolase / Q000145:classification / Q000235:genetics*; D005798:Genes, Bacterial; D005947:Glucose; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D014158:Transcription, Genetic
chemical_list: D004269:DNA, Bacterial; D005634:Fructose-Bisphosphate Aldolase; D005947:Glucose