Selective killing of human monocytes by an immunotoxin containing partially denatured mistletoe lectin I.
The in vitro activity of an immunotoxin (IT) prepared by conjugating the monocyte specific RoMo-1 monoclonal antibody and partially inactivated Mistletoe lectin I (dMLI) containing inactivated B chain has been initially characterized. It is shown that while not affecting other mononuclear cells viability, this IT is capable of selectively destroying human monocytes after 24 h exposure thus resulting in the abrogation of monocyte support for PHA reactivity in mononuclear cell preparations. Therefore it seems to be possible to use this non-sugar binding holotoxin for immunotoxin preparation.
Schütt
C
C
Department of Medical Immunology, Ernst-Moritz-Arndt-Universität Greifswald, German Democratic Republic.
Pfüller
U
U
Siegl
E
E
Walzel
H
H
Franz
H
H
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D003602:Cytotoxicity, Immunologic; D006801:Humans; D000922:Immunotoxins / Q000494:pharmacology*; D066298:In Vitro Techniques; D037102:Lectins / Q000494:pharmacology; D009000:Monocytes / Q000187:drug effects* / Q000276:immunology; D028321:Plant Preparations; D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological / Q000494:pharmacology*
D000911:Antibodies, Monoclonal; D000922:Immunotoxins; D037102:Lectins; D028321:Plant Preparations; D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological; C009674:ribosome inactivating protein, Viscum