[Affinity modification of DNA-dependent RNA-polymerase from phage T7 with 5'-p-fluorosulfonylbenzoyl adenosine: the effect of modification on the interaction with substrates].
T7 RNA polymerase, covalently modified with 5'-p-fluorosulfonylbenzoyl adenosine, looses the ability of binding the promoter (pGEM-2 plasmid) and poly(dC) template as well as the initiating nucleoside triphosphate (GTP). However the enzyme retains the unspecific binding with DNA fragments of considerable length.
Tunitskaia
V L
VL
Luchin
S V
SV
Memelova
L V
LV
Liakhov
D L
DL
Rechinskiĭ
V O
VO
Kochetkov
S N
SN
D000241:Adenosine / Q000031:analogs & derivatives* / Q000378:metabolism; D000345:Affinity Labels; D001667:Binding, Competitive; D004247:DNA / Q000235:genetics / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D010957:Plasmids; D011066:Poly C / Q000378:metabolism; D011401:Promoter Regions, Genetic; D013379:Substrate Specificity; D013604:T-Phages / Q000201:enzymology*; D013698:Templates, Genetic
D000345:Affinity Labels; C023826:poly(dC); D011066:Poly C; C010959:5'-(4-fluorosulfonylbenzoyl)adenosine; D006160:Guanosine Triphosphate; D004247:DNA; D012321:DNA-Directed RNA Polymerases; D000241:Adenosine
Affinnaia modifikatsiia DNK-zavisimoĭ RNK-polimerazy faga T7 5'-p-ftorsul'fonilbenzoiladenozinom: vliianie modifikatsii na vzaimodeĭstvie s substratami.