[Determination of the biliary excretion of reduced and oxidized glutathione].
An enzymatic method is presented for the detection of biliary excretion of reduced (GSH) and oxidized (GSSG) glutathione. The biliary excretion of GSH is 65.63 +/- 7.23 nmoles/kg b.w. x min and of GSSG 5.90 +/- 0.64 nmoles/kg b.w. x min in 55 day-old female rats, respectively. The recovery is 97.39 +/- 0.40% for GSH and 91.05 +/- 2.30% for GSSG. Cysteine which can be detected in bile, does not influence the accuracy of the results.
Kretzschmar
M
M
Fleck
C
C
Klinger
W
W
D000818:Animals; D001646:Bile / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D005260:Female; D005978:Glutathione / Q000032:analysis* / Q000378:metabolism; D010084:Oxidation-Reduction; D051381:Rats
D005978:Glutathione
Bestimmung der biliären Exkretion des reduzierten und oxidierten Glutathions.