Diagnostic and prognostic significance of myelomonocytic cell surface antigens in acute myeloid leukaemia.
Thirty-six cases of acute myeloid leukaemia (AML) were tested with a large battery of monoclonal antibodies (moAbs) detecting surface markers normally expressed by myelomonocytic, T and B lymphoid, megakaryocytic and erythroid lineages. Differences in antigenic expression were observed among the various FAB subgroups: HLA-class II molecules were found in almost all AML cases but not in the promyelocytic subgroup (M3); CD14 and CD36 antigens were detected in monocytic leukaemias (M4 and M5); the CD34 moAb (MY10) recognizing an epitope described on myeloid stem cells was positive in 88% of the M1 and 80% of the M3 cases. By a multivariate analysis, only the CD14b (MY4) discriminated significantly between M1-M2 and M4-M5 subgroups. Using Cox's model to assess the prognostic importance of variables including immunophenotyping on survival, we undertook a one by one analysis and found that the presence of CD17 antigen predicted for a shorter survival (P = 0.03). In addition this marker appeared more significant than other clinical and biological parameters.
Merle-Beral
H
H
Département d'Hématologie et Unité, Claude Bernard C20, Hôpital, Pitié-Salpêtrière, Paris, France.
Nguyen Cong Duc
L
L
Leblond
V
V
Boucheix
C
C
Michel
A
A
Chastang
C
C
Debre
P
P
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis*; D001853:Bone Marrow / Q000276:immunology; D005260:Female; D006801:Humans; D015479:Leukemia, Myelomonocytic, Acute / Q000175:diagnosis / Q000276:immunology* / Q000401:mortality; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D012737:Sex Factors
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000954:Antigens, Surface