Purification and characterization of two human liver carboxylesterases.
1. Two carboxylesterases (EC 3.1.1.1) purified from human livers were distinguished by pI (isoelectric point), nondenaturing polyacrylamide gel electrophoresis, molecular weight, catalytic activity, N-terminus and immunological cross-reactivity. 2. The low pI carboxylesterase has not been reported previously. 3. Numerous bands seen when each enzyme was focused on analytical IEF gels could not be separated. 4. When sections of the band pattern was refocused, the original complete band pattern was generated. 5. Both the mid and low pI carboxylesterases had catalytic activity for xenobiotics as well as medium and long chain fatty acid esters.
Ketterman
A J
AJ
University of Queensland Department of Medicine, Princess Alexandra Hospital, Brisbane, Australia.
Bowles
M R
MR
Pond
S M
SM
D000595:Amino Acid Sequence; D002265:Carboxylic Ester Hydrolases / Q000276:immunology / Q000302:isolation & purification*; D002384:Catalysis; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability; D006026:Glycoside Hydrolases; D006801:Humans; D015151:Immunoblotting; D007525:Isoelectric Focusing; D007526:Isoelectric Point; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D013379:Substrate Specificity
D002265:Carboxylic Ester Hydrolases; D006026:Glycoside Hydrolases