The use of hybrid isoelectric focusing for the detection of polymorphic proteins in blood stains.
alpha1-Antitrypsin (Pi), transferrin (Tf) and orosomucoid (ORM) were determined in bloodstain extracts by isoelectric focusing (IEF) with carrier ampholytes (CA) and also with a mixture of immobilines (HIEF). HIEF yields superior results from proteins typing in bloodstain extracts, since phenotypes are better distinguished and the bands are straighter and sharper. Also the sensitivity of HIEF is similar to IEF with CA.
Lopez-Rodriguez
I
I
Department of Legal Medicine, University of Santiago, Spain.
Muñoz-Barus
J I
JI
Carracedo
A
A
Montiel
M D
MD
Concheiro
L
L
D001801:Blood Stains; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing / Q000379:methods*; D009961:Orosomucoid / Q000032:analysis*; D014168:Transferrin / Q000032:analysis*; D000515:alpha 1-Antitrypsin / Q000032:analysis*
D009961:Orosomucoid; D014168:Transferrin; D000515:alpha 1-Antitrypsin