Evidence of steroid receptors in human osteoblast-like cells.
A nuclear binding assay was used to demonstrate steroid receptors in normal human osteoblast-like cells. Nuclear binding of [3H]estradiol was found in 27 of 30 (90%) cell strains and nuclear binding of [3H]androgen was identified in 21 of 25 (84%) separate osteoblast cell strains. The nuclear binding was saturable and steroid-specific. Estrogen and androgen receptor gene expression was confirmed by RNA blot analysis. These data suggest that circulating sex steroids may act directly on normal human osteoblasts through receptor-mediated mechanisms.
Colvard
D
D
Mayo Clinic, Rochester, MN 55905.
Spelsberg
T
T
Eriksen
E
E
Keeting
P
P
Riggs
B L
BL
D000728:Androgens / Q000378:metabolism / Q000494:pharmacology; D002478:Cells, Cultured; D004967:Estrogens / Q000378:metabolism / Q000494:pharmacology; D015870:Gene Expression; D006801:Humans; D015151:Immunoblotting; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D011944:Receptors, Androgen / Q000235:genetics / Q000378:metabolism; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism; D011987:Receptors, Steroid / Q000235:genetics / Q000378:metabolism*
D000728:Androgens; D004967:Estrogens; D011944:Receptors, Androgen; D011960:Receptors, Estrogen; D011987:Receptors, Steroid