Fragmented fibronectin and other synovial fluid proteins in chronic arthritis: their relation to immune complexes.
Fibronectin, an opsonic glycoprotein has been shown to exist in fragmented forms in serum and synovial fluid. Some fragments in synovial fluid appear to be polyethylene glycol (PEG) precipitable, suggesting incorporation into immune complexes (IC). PEG precipitation, SDS-PAGE and immunoblotting were used to determine whether PEG precipitable fragments are real or artefactual. Disease specificity of fragmentation and IC incorporation of fibronectin and other proteins were also studied using these techniques. PEG precipitable fragments do not appear to be artefactual, although some fibronectin fragments are cryoprecipitable. Protein fragments showed similar distributions in whole serum and synovial fluid, disease specific differences being confined to PEG precipitates. Rheumatoid arthritis (RA) synovial fluid PEG precipitates displayed the greatest array of fragmented immunoglobulins and fibronectin. No PEG precipitates contained albumin fragments. Protein fragments in IC may impair their effective removal from RA joints. Accumulated IC could lead to tissue damage via complement activation.
Griffiths
A M
AM
Department of Rheumatology, Medical College of St. Bartholomew's Hospital, West Smithfield, London, UK.
Herbert
K E
KE
Perrett
D
D
Scott
D L
DL
D000328:Adult; D000368:Aged; D000936:Antigen-Antibody Complex / Q000502:physiology*; D001168:Arthritis / Q000097:blood / Q000276:immunology* / Q000503:physiopathology; D011232:Chemical Precipitation; D005260:Female; D005353:Fibronectins / Q000032:analysis* / Q000097:blood; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000032:analysis* / Q000097:blood; D011092:Polyethylene Glycols; D012709:Serum Albumin / Q000032:analysis; D013048:Specimen Handling; D013582:Synovial Fluid / Q000032:analysis*
D000936:Antigen-Antibody Complex; D005353:Fibronectins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D010446:Peptide Fragments; D012709:Serum Albumin; D011092:Polyethylene Glycols