title: Morphometrical and cell kinetic studies of normal human colorectal mucosa. Comparison between the proximal and the distal large intestine.
abstract: Morphometric and cell kinetic studies were performed on normal mucosa in 30 specimens of surgically resected colorectum. The average crypt length was 372 +/- 57 microns in the proximal colon and 506 +/- 65 microns in the distal colon, and the average cell numbers per crypt column in these regions were 76.4 +/- 8.9 and 80.3 +/- 8.8, respectively. In the proximal and distal colon, goblet cells constituted 23.3 +/- 3.4% and 25.2 +/- 4.0%, respectively, of the total cells in each crypt, and in both regions entero-chromaffin cells constituted about 1%. The labeling indices of the proximal and distal colon were 9.4 +/- 3.9% and 8.2 +/- 2.2%, respectively. Combined microautoradiography and periodic acid-Schiff reaction on selected cases revealed that the labeling indices of columnar cells and goblet cells were 11.0 +/- 2.0% and 7.0 +/- 3.2%, respectively. It was suggested that the increased number of larger goblet cells in crypts of the sigmoid colon and rectum resulted in an increase of crypt length and a decrease in the labeling index. These results provide information on the morphological and cell kinetic characteristics of normal mucosa of the colon and rectum.
authors.affiliation: First Department of Pathology, Hamamatsu University School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Arai
authors.forename: I
authors.initials: I
authors.lastname: Kino
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002453:Cell Cycle; D002838:Chromaffin System / Q000166:cytology*; D003106:Colon / Q000166:cytology*; D004759:Enterochromaffin Cells / Q000166:cytology*; D006801:Humans; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000166:cytology*; D008297:Male; D008875:Middle Aged; D012007:Rectum / Q000166:cytology*; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
chemical_list: D014316:Tritium; D013936:Thymidine