The use of targetted liposomes to isolate cells bearing immunoglobulin receptors.
A new method of mouse B cell isolation has been developed. It is based on the differences in buoyant density of free lymphocytes and cells bound within liposome-cellular complexes (LCC). LCC arise during the interaction of B cells bearing surface immunoglobulins (Ig) with liposomes loaded with affinity purified rabbit antibodies to mouse IgM/IgG. Advantage was taken of the fact that LCC can be selectively removed from other cell populations by differential flotation in Ficoll gradients. Because of their lower density the LCC did not sediment under conditions where free lymphocytes and complexes of the liposomes with dead or damaged lymphocytes did sediment. The method was highly specific and the recovery of B cells was about 50%. B cells isolated with the use of targetted liposomes retained viability and functional activity. Theoretically, liposomes loaded with antibodies to any cell surface marker could be used for the isolation of cells bearing such markers.
Golubev
A
A
Laboratory of Immunoglobulin Biosynthesis, Moscow Research Institute for Viral Preparations, U.S.S.R.
Sidorova
E
E
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D007075:Immunoglobulin M / Q000276:immunology; D066298:In Vitro Techniques; D008081:Liposomes; D051379:Mice; D011947:Receptors, Antigen, B-Cell / Q000276:immunology
D007075:Immunoglobulin M; D008081:Liposomes; D011947:Receptors, Antigen, B-Cell