Release of hemoglobin and potassium from human red blood cells treated with Triton X-100 under the critical micellar concentration.
The action of detergents is thought to be connected primarily with micelle formation. However, detergent monomers can also effect biological systems. It was found in this study that human red blood cells can be disintegrated with Triton X-100 non-ionic detergent at a concentration of 0.007%, lower than the critical micellar concentration (CMC). The time dependent release of hemoglobin and potassium was detected at 37 degrees C and both were sigmoid in character. Although potassium was released faster than hemoglobin, a cooperative relationship between potassium and hemoglobin within the intact red blood cell is suggested by this observation.
Bogner
P
P
Department of Clinical Chemistry, University Medical School, Pecs, Hungary.
Kellermayer
M
M
Hazlewood
C F
CF
D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D006454:Hemoglobins / Q000493:pharmacokinetics*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D017830:Octoxynol; D011092:Polyethylene Glycols; D011188:Potassium / Q000493:pharmacokinetics*
D006454:Hemoglobins; D011092:Polyethylene Glycols; D017830:Octoxynol; D011188:Potassium