Analysis of ecdysteroids in Onchocerca gibsoni, O. volvulus and nodule tissues.
Free ecdysteroids were detected in Onchocerca gibsoni, in tissues constituting O. volvulus and O. gibsoni nodules and in unrelated bovine tissues. Ecdysone and 20-hydroxyecdysone were identified by HPLC-RIA and GC/MS(SIM). The concentration of free ecdysteroids in the nodule tissue immediately surrounding the parasites was at least an order of magnitude higher than that detected in the worms themselves, or in adjacent nodular tissues or other bovine tissues.
Mercer
J G
JG
Department of Biochemistry, University of Liverpool, U.K.
Barker
G C
GC
Howells
R E
RE
Rees
H H
HH
D000003:Abattoirs; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003238:Connective Tissue / Q000032:analysis*; D026461:Ecdysteroids; D007447:Invertebrate Hormones / Q000302:isolation & purification*; D008321:Mammary Glands, Animal / Q000032:analysis*; D009854:Onchocerca / Q000032:analysis*
D026461:Ecdysteroids; D007447:Invertebrate Hormones