[Recovery from potentially lethal damage of irradiated L5178Y-R and L5178Y-S cells: the effect of temperature and benzamide].
Mouse lymphoma L5178 Y-S and Y-R cells differing in radiosensitivity by 1.5 times were treated with benzamide, an inhibitor of poly(ADP-ribosylation), for 24 h before and 18 h after X-irradiation, and incubated after irradiation at 25 degrees C and 37 degrees C. Clonogenic capacity of LY-S cells incubated at 25 degrees C exceeded that of the same cells incubated at 37 degrees C; the clonogenic capacity of LY-R cells did not vary with the postirradiation incubation temperature. Benzamide increased equally the radiosensitivity of LY-R cells incubated at both temperatures, whereas that of LY-S cells was only increased at 37 degrees C. Repair of potentially lethal damages to LY-S cells incubated at 25 degrees C was independent of the effectiveness of poly(ADP-ribosylation).
Afanas'ev
G G
GG
Burachevska
I
I
Godlevska
E
E
Ludvitska
A
A
Shumel'
I
I
D000818:Animals; D001549:Benzamides / Q000494:pharmacology*; D002460:Cell Line; D004260:DNA Repair / Q000187:drug effects / Q000528:radiation effects*; D004273:DNA, Neoplasm / Q000187:drug effects / Q000528:radiation effects*; D066298:In Vitro Techniques; D007940:Leukemia L5178 / Q000473:pathology*; D007942:Leukemia, Experimental / Q000473:pathology*; D051379:Mice; D011064:Poly Adenosine Diphosphate Ribose / Q000378:metabolism; D011836:Radiation Tolerance; D013696:Temperature
D001549:Benzamides; D004273:DNA, Neoplasm; D011064:Poly Adenosine Diphosphate Ribose
Vosstanovlenie ot potentsial'no letal'nykh povrezhdeniĭ obluchennykh kletok L5178Y-R i L5178Y-S: vliianie temperatury i benzamida.