Measurement of K vitamins in animal tissues by high-performance liquid chromatography with fluorimetric detection.
A highly sensitive method for measuring endogenous phylloquinone and menaquinones in animal tissues was developed, based on high-performance liquid chromatography with coulometric reduction and fluorimetric detection, following extraction from tissue homogenate and purification on a Sep-Pak silica cartridge followed by thin-layer chromatography. The detection limits of phylloquinone, menaquinone-4, -6, -10 and -13 were 40, 40, 50, 70 and 80 pg/g in rat liver, respectively.
Hirauchi
K
K
Shionogi Research Laboratories, Shionogi & Co, Ltd., Osaka, Japan.
Sakano
T
T
Notsumoto
S
S
Nagaoka
T
T
Morimoto
A
A
Fujimoto
K
K
Masuda
S
S
Suzuki
Y
Y
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D013050:Spectrometry, Fluorescence; D014018:Tissue Distribution; D014812:Vitamin K / Q000032:analysis* / Q000493:pharmacokinetics
D014812:Vitamin K