title: A rapid procedure for the purification of IgA1 and IgA2 subclasses from normal human serum using protein G and jackfruit lectin (jacalin) affinity chromatography.
abstract: Immunoglobulin A (IgA) from pooled normal human sera was purified using antibody and protein G affinity chromatography and gel filtration high-pressure liquid chromatography (HPLC). This high purity product was separated into IgA1 and IgA2 subclasses utilizing the agarose-bound lectin 'jacalin'. Evaluation of product homogeneity by immunological testing confirmed greater than 95% purity. The total IgA1 and IgA2 recovered from sera was approximately 26% of the initial antibody present.
authors.affiliation: Protein Chemistry Section, Canadian Red Cross Society, Ottawa, Ontario.
authors.forename: M
authors.initials: M
authors.lastname: Haun
authors.forename: B
authors.initials: B
authors.lastname: Incledon
authors.forename: P
authors.initials: P
authors.lastname: Alles
authors.forename: S
authors.initials: S
authors.lastname: Wasi
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods*; D002847:Chromatography, Agarose; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D006801:Humans; D007070:Immunoglobulin A / Q000145:classification / Q000302:isolation & purification*; D037102:Lectins; D009419:Nerve Tissue Proteins; D037121:Plant Lectins
chemical_list: C029152:G-substrate; D007070:Immunoglobulin A; D037102:Lectins; D009419:Nerve Tissue Proteins; D037121:Plant Lectins; C043696:jacalin