Stimulation of forward motility of goat cauda epididymal spermatozoa by a serum glycoprotein factor.
Blood sera of humans, rats, goats, and buffalo have been shown to possess a forward motility-stimulating factor (FMSF) that markedly stimulated goat cauda epididymal sperm forward motility, as assayed by a microscopic method in the presence of epididymal plasma (1.2 mg protein/ml) that had sufficient anti-sticking activity to eliminate the possibility of cell-sticking artifacts in motility assays. The specific activity of FMSF was greatest in buffalo blood serum compared to the sera of the other species. Buffalo serum at a concentration as low as 8.5 mg protein/ml induced forward motility in nearly 45% of the cells. The buffalo serum FMSF was heat-stable, nondialyzable, and sensitive to the action of trypsin. Purified proteins--casein, serum albumin, ovalbumin, myoglobin, and beta-lactoglobulin--showed little or relatively low FMSF activity. FMSF is a glycoprotein, as it binds with high affinity to concanavalin A-agarose. A major portion of the serum protein (approx. 70%) did not bind to the affinity matrix, and this unretained serum protein fraction showed little FMSF activity. The FMSF activity of buffalo serum was confirmed by estimating sperm forward motility spectrophotometrically: an objective method of assessing sperm motility.
Mandal
M
M
Indian Institute of Chemical Biology, Jadavpyur, Calcutta.
Banerjee
S
S
Majumder
G C
GC
D000818:Animals; D002020:Buffaloes; D006023:Glycoproteins / Q000097:blood / Q000494:pharmacology*; D006041:Goats / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D011506:Proteins / Q000494:pharmacology*; D051381:Rats; D013081:Sperm Motility / Q000187:drug effects*
D006023:Glycoproteins; D011506:Proteins; C029700:sperm forward motility protein, Bos taurus