title: Interaction of type I collagen fibrils with phospholipid vesicles.
abstract: Type I collagen fibrils interact with phosphatidylcholine and phosphatidylglycerol vesicles. Fluorescence polarization of (1,6-diphenyl-1,3,5-hexatriene) DPH-labeled vesicles, circular dichroism and differential scanning calorimetry studies have been performed. The protein-lipid interaction produces a decrease of the enthalpy of the phospholipid phase transition. Positive charges of lysine residues of the protein are involved in the interaction as experiments with succinylated collagen show. The kinetic parameters and the extent of the fibrillogenesis of collagen are modified by the phospholipid vesicles.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad Complutense, Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Martinez del Pozo
authors.forename: M
authors.initials: M
authors.lastname: Onaderra
authors.forename: J
authors.initials: J
authors.lastname: Laynez
authors.forename: J G
authors.initials: JG
authors.lastname: Gavilanes
mesh_terms: D000818:Animals; D002152:Calorimetry, Differential Scanning; D003094:Collagen; D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; D010743:Phospholipids; D051381:Rats; D013050:Spectrometry, Fluorescence; D013623:Tail; D013710:Tendons; D013816:Thermodynamics
chemical_list: D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; D010743:Phospholipids; D003094:Collagen