[Influence of reduced glutathione on changes in the activity of phospholipase C induced by 4-hydroxynonenal].
4-Hydroxynonenal (HNE) is a major end-product of lipid peroxidation. 1 mM HNE inhibits the activity of liver phospholipase C (PL-C) and this effect is prevented by 1 mM GSH; on the contrary GSH is unable to counteract the stimulation of PL-C induced by a low concentration of HNE (100 nM). Other hydroxyalkenals are able to stimulate PL-C at low doses (micromolar or less), the most effective being 4-hydroxyoctenal which acts at picomolar doses. The lack of a correlation between the chain length of the aldehydes used and the degree of PL-C stimulation seems to exclude the possibility that their effect could be due to an aspecific solvent action toward the phosphatidylinositol-4,5-diphosphate used as substrate for the enzymatic assay.
Garramone
A
A
Fidale
F
F
Rossi
M A
MA
D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D005978:Glutathione / Q000494:pharmacology*; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D010738:Type C Phospholipases / Q000378:metabolism*
D000447:Aldehydes; D010738:Type C Phospholipases; D005978:Glutathione; C027576:4-hydroxy-2-nonenal
Influenza del glutatione ridotto sulle modificazioni dell'attività della fosfolipasi C indotte dal 4-idrossinonenale.