Enhancement of gentamicin-induced inhibition of phosphatidylinositol hydrolysis in rabbit renal proximal tubular brush-border membrane by furosemide.
Gentamicin inhibited phosphatidylinositol (PI) hydrolysis catalyzed by rabbit renal phospholipase C in a dose-dependent manner. The inhibition was potentiated by furosemide in a dose-dependent manner. Furosemide may enhance gentamicin nephrotoxicity by potentiating the inhibitory effect of gentamicin on brush-border membrane PI degradation.
Nakahama
H
H
First Department of Medicine, Osaka University Medical School, Japan.
Shin
S
S
Moriyama
T
T
Kakihara
M
M
Fukuhara
Y
Y
Orita
Y
Y
Kamada
T
T
D000818:Animals; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D005665:Furosemide / Q000494:pharmacology*; D005839:Gentamicins / Q000494:pharmacology*; D006868:Hydrolysis / Q000187:drug effects; D066298:In Vitro Techniques; D007687:Kidney Tubules, Proximal / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008871:Microvilli / Q000187:drug effects / Q000201:enzymology; D010716:Phosphatidylinositols / Q000378:metabolism*; D011817:Rabbits; D010738:Type C Phospholipases / Q000502:physiology
D005839:Gentamicins; D010716:Phosphatidylinositols; D005665:Furosemide; D010738:Type C Phospholipases