Chlamydia trachomatis 60 kDa cysteine rich outer membrane protein: sequence homology between trachoma and LGV biovars.
An 820 bp AccI-PstI fragment of the 60 kDa cysteine rich outer membrane protein (CrP) gene from C. trachomatis serovar L1 was used as a probe to locate the 60 kDa CrP gene of a recent serovar B trachoma isolate (Jali 20/OT). The probe hybridized to a single 1.8 kb SpeI fragment in Southern blot analyses of different restriction endonuclease digests of C. trachomatis serovar B DNA. This fragment was ligated into Lambda Zap II arms and Bluescript SK(-) recombinants, released by infection with the helper phage R408, were used as template for DNA sequence determination. Sequence analysis demonstrated a very high level of homology between the Jali 20/OT 60 kDa CrP and the previously published serovar L1 60 kDa CrP with only 8 out of 507 amino acid substitutions between the two proteins.
Watson
M W
MW
Department of Microbiology, University of Southampton Medical School, Southampton General Hospital, U.K.
Lambden
P R
PR
Ward
M E
ME
Clarke
I N
IN
D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D002692:Chlamydia trachomatis / Q000235:genetics*; D003545:Cysteine; D004269:DNA, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D006801:Humans; D008219:Lymphogranuloma Venereum / Q000382:microbiology*; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014141:Trachoma / Q000382:microbiology*
D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; D003545:Cysteine