title: Ragged-red fibres detected in paraffin sections by a monoclonal antibody to inner mitochondrial membrane.
abstract: An immunohistochemical method is reported using the M-II68 monoclonal antibody, which detects mitochondrial accumulations ("ragged-red fibres") in routinely processed (formalin-fixed, paraffin-embedded) muscle tissue. Ten cases with electron-microscopically and histochemically proven mitochondrial myopathy featured 4% to 24% ragged-red fibres. In a series of 50 muscle biopsies without mitochondrial myopathy, scattered ragged-red fibres (less than 0.1%) were present in a few normal and pathological muscles. The immunohistochemical method is specific for mitochondria, does not require frozen tissue and permits rapid examination of large areas.
authors.affiliation: Institut für Hirnforschung, Universität Tübingen, Federal Republic of Germany.
authors.forename: W
authors.initials: W
authors.lastname: Paulus
authors.forename: J
authors.initials: J
authors.lastname: Peiffer
authors.forename: W
authors.initials: W
authors.lastname: Roggendorf
authors.forename: K H
authors.initials: KH
authors.lastname: Wiedmann
authors.forename: J M
authors.initials: JM
authors.lastname: Schröder
mesh_terms: D000911:Antibodies, Monoclonal; D006801:Humans; D007150:Immunohistochemistry; D008931:Mitochondria, Muscle / Q000276:immunology / Q000473:pathology*; D009135:Muscular Diseases / Q000473:pathology*
chemical_list: D000911:Antibodies, Monoclonal