Expression of rat hepatocyte plasma membrane antigens in hybrid clones: assignment of genes coding for two antigens of the basolateral domain to chromosomes 11 and 13.
Expression of three rat hepatocyte plasma membrane antigens defined by monoclonal antibodies (mAbs) was examined by immunofluorescence in mouse hepatoma x rat hepatocyte hybrid clones segregating rat chromosomes. The antigen defined by mAb B1, a marker of the lateral domain of the hepatocyte plasma membrane in vivo, was expressed in hybrids retaining the rat chromosome 11. The antigen defined by mAb A39, mainly located on the sinusoidal (basal) domain of the plasma membrane in vivo, was expressed when chromosome 13 was present. The genes coding for these two antigens were thus assigned to chromosomes 11 and 13, respectively. The antigen defined by mAb B10, exclusively located on the canalicular (apical) domain of the plasma membrane in vivo, was not expressed in most hybrid clones, and the chromosome location of the gene could not be determined.
Perrotez
C
C
Laboratoire de Biologie Cellulaire, INSERM U24, Faculté de Médecine Xavier-Bichat, Paris, France.
Maurice
M
M
Feldmann
G
G
Szpirer
C
C
Szpirer
J
J
Parent
I
I
Cassio
D
D
Levan
G
G
D000818:Animals; D000954:Antigens, Surface / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism / Q000648:ultrastructure; D002874:Chromosome Mapping; D002875:Chromosomes / Q000648:ultrastructure; D015870:Gene Expression; D006822:Hybrid Cells / Q000166:cytology / Q000276:immunology / Q000648:ultrastructure; D008099:Liver / Q000166:cytology* / Q000276:immunology / Q000648:ultrastructure; D008114:Liver Neoplasms, Experimental / Q000235:genetics / Q000276:immunology / Q000473:pathology; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D011919:Rats, Inbred Strains
D000954:Antigens, Surface; D008565:Membrane Proteins