title: Simultaneous identification of amphetamine and methamphetamine using solid-phase extraction and gas chromatography/nitrogen phosphorous detection or gas chromatography/mass spectrometry.
abstract: A method for the simultaneous detection and quantitation of amphetamine and methamphetamine in urine is described. Using solid-phase extraction, amphetamine, methamphetamine, and n-propylamphetamine (internal standard) are extracted from urine samples. Drugs in their free form are identified using gas chromatography/nitrogen-phosphorous detection (GC/NPD), whereas their heptafluorobutyric anhydride derivatives are detected by gas chromatography/mass spectrometry (GC/MS) in the selected ion monitoring (SIM) mode. Limits of detection for both amphetamine and methamphetamine are approximately 35 ng/mL. The procedure is simple, rapid, and suitable for a large number of specimens (25 or more).
authors.affiliation: Los Angeles County Sheriff's Department, Scientific Services Bureau, California 90057-2494.
authors.forename: R W
authors.initials: RW
authors.lastname: Taylor
authors.forename: S D
authors.initials: SD
authors.lastname: Le
authors.forename: S
authors.initials: S
authors.lastname: Philip
authors.forename: N C
authors.initials: NC
authors.lastname: Jain
mesh_terms: D000661:Amphetamine / Q000652:urine*; D005069:Evaluation Studies as Topic; D005410:Flame Ionization; D008401:Gas Chromatography-Mass Spectrometry; D008694:Methamphetamine / Q000652:urine*; D013048:Specimen Handling / Q000379:methods
chemical_list: D008694:Methamphetamine; D000661:Amphetamine