A method for the simultaneous analysis of unconjugated and glycine-conjugated bile acids by capillary gas-liquid chromatography.
A method for the simultaneous analysis of unconjugated and glycine-conjugated bile acids by means of capillary gas-liquid chromatography without need for prior deconjugation is described. The method involves: i) the use of an aluminum-clad fused-silica capillary column coated with a very thin film (0.1 micron) of a highly thermostable bonded and crosslinked methyl polysiloxane, and ii) the analysis of the bile acids as their methyl ester-dimethylethylsilyl ether derivatives. This method, used to separate the major free and glycine-conjugated bile acids from human gall bladder bile, should be applicable for the analysis of other biological fluids.
Iida
T
T
College of Engineering, Nihon University, Fukushima-ken, Japan.
Itoh
T
T
Hagiwara
K
K
Chang
F C
FC
Goto
J
J
Nambara
T
T
D001647:Bile Acids and Salts / Q000032:analysis* / Q000378:metabolism; D002849:Chromatography, Gas / Q000379:methods*; D005704:Gallbladder / Q000032:analysis; D005998:Glycine / Q000378:metabolism*; D006801:Humans
D001647:Bile Acids and Salts; D005998:Glycine