Rat monoclonal antibodies to murine IgM determinants: application to antibody purification.
Two monoclonal rat antibodies (RAM11 and RAM12) were raised against mouse IgM antibodies. These 2 antibodies were isotype-specific since they bound murine IgM but not IgG or IgA antibodies; moreover, sera from mouse strains possessing different allotypic determinants inhibited the binding of RAM11 and RAM12. Both antibodies can be readily purified from culture supernatants on protein G-agarose (but not on protein A-agarose). After coupling to Sepharose 4B, RAM11 provided a useful affinity chromatography support for the purification of murine IgM antibodies from biological fluids.
Monestier
M
M
Institute of Molecular Immunology, University of Medicine and Dentistry of New Jersey, Newark.
Debbas
M E
ME
Zhou
S L
SL
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002846:Chromatography, Affinity; D007132:Immunoglobulin Isotypes / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000302:isolation & purification*; D051379:Mice; D009419:Nerve Tissue Proteins; D051381:Rats; D013205:Staphylococcal Protein A
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; C029152:G-substrate; D007132:Immunoglobulin Isotypes; D007075:Immunoglobulin M; D009419:Nerve Tissue Proteins; D013205:Staphylococcal Protein A