title: Biosynthesis of elsamicin A, a novel antitumor antibiotic.
abstract: The 1H noise-decoupled 13C-nmr spectrum of elsamicin A [1] prepared from the cultures of an unknown actinomycete species (ATCC 39417) supplemented with [1-13C]acetate and [2-13C]acetate showed enrichment of all 19-carbons in the aglycone. In addition, L-[methyl-13C]methionine- and D-[1-13C]glucose-supplemented cultures enriched the carbons of the two methyl groups on the disaccharide moiety and the C-1 carbons of the disaccharide, respectively. The results demonstrated that the aglycone of elsamicin A is derived entirely from acetate and the disaccharide portion is biosynthesized from two units of glucose and methionine.
authors.affiliation: Bristol-Myers Company, Pharmaceutical Research and Development Division, Wallingford, Connecticut 06492.
authors.forename: K S
authors.initials: KS
authors.lastname: Lam
authors.forename: J A
authors.initials: JA
authors.lastname: Veitch
authors.forename: S
authors.initials: S
authors.lastname: Forenza
authors.forename: C M
authors.initials: CM
authors.lastname: Combs
authors.forename: K L
authors.initials: KL
authors.lastname: Colson
mesh_terms: D000192:Actinomycetales / Q000254:growth & development / Q000378:metabolism; D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000302:isolation & purification; D000903:Antibiotics, Antineoplastic / Q000096:biosynthesis*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; C049904:elsamicin A