title: The cell barrier effect in rabbit corneas kept under eye bank conditions.
abstract: The influence of endothelial cell pumping and of cell barrier functions on cultivated corneas has been studied by comparing the rate of weight increase in freeze-treated, ouabain-treated and fresh corneas. Inhibition of the endothelial cell pump activity increased the swelling rate from 0.5% of start weight per h (fresh corneas) to about 2% per h (ouabain-treated corneas), as measured after 24 h in culture medium. The cell barrier effect was estimated by comparing the swelling rate of ouabain-treated corneas after 24 h, and the swelling rate of freeze-treated corneas with the same swelling pressure. The values are 2 and 9.5% per h, respectively. The swelling rate of freeze-treated corneas with different button diameters was found to increase with decreasing corneal button diameter.
authors.affiliation: University Eye Department, Rikshospitalet, University of Oslo, Norway.
authors.forename: W
authors.initials: W
authors.lastname: Medin
authors.forename: M
authors.initials: M
authors.lastname: Davanger
mesh_terms: D000818:Animals; D003315:Cornea / Q000166:cytology* / Q000187:drug effects; D003470:Culture Media; D004728:Endothelium, Corneal / Q000166:cytology* / Q000187:drug effects; D005125:Eye Banks; D005615:Freezing; D010042:Ouabain / Q000494:pharmacology; D011817:Rabbits; D014015:Tissue Banks
chemical_list: D003470:Culture Media; D010042:Ouabain