[Isolation and purification of biopolymers using an affinity chromatography method. IX. Preparation, properties and use of affinity adsorbents with immobilized polypeptide fragments of collagen].
Synthesis and properties of new affinity adsorbents with immobilized polypeptide fragments of collagen molecule (alpha-chains, beta-components, cyanogen bromide peptides) were described. Adsorbents with alpha-chains and alpha 1CB7-peptide had fibronectin binding capacity 1.5-2.0 times higher than commercial gelatin-Sepharose. Commercial production of highly purified fibronectin from human plasma using affinity chromatography on immobilized individual alpha-chains of collagen was developed.
Mitina
V Kh
VKh
Frantsuzova
N A
NA
Kliashitskiĭ
V A
VA
Krasnopol'skiĭ
Iu M
IuM
Sennikov
G A
GA
Svets
V I
VI
Orekhovich
V N
VN
D000818:Animals; D001704:Biopolymers; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003094:Collagen; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D046911:Macromolecular Substances; D010446:Peptide Fragments; D051381:Rats; D013056:Spectrophotometry, Ultraviolet
D001704:Biopolymers; D005353:Fibronectins; D046911:Macromolecular Substances; D010446:Peptide Fragments; D003094:Collagen
Vydelenie i ochistka biopolimerov metodom affinnoĭ khromatografii. IX. Poluchenie, svoĭstva i primenenie affinnykh adsorbentov s immobilizovannymi polipeptidnymi fragmentami kollagena.