[Comparison of the common fragile sites and G-banding patterns of chromosomes among rhesus monkeys, white-eyebrow gibbons and human being].
The peripheral lymphocyte cultures from Rhesus monkeys (Macaca mulatta), White-eyebrow gibbons (Hylobates hoolock) and human being were treated with BrdU (bromodeoxyuridine), FrdU (fluorodeoxyuridine) and MTX (methotrexate). A number of 22 Common fragile sites for Rhesus monkeys and 20 for white-eyebrow gibbons were found. Chromosome homology and the relationship between fragile sites and chromosomal evolution were discussed. Several common fragile sites of human chromosomes have been found at the homologous chromosomal regions in Rhesus monkeys as well as in White-eyebrow gibbons. Furthermore, the fragile sites at 1q14, 1p32 of Rhesus monkey chromosomes may correspond to the homologous fragile sites at 1p21, 1q31 of White-eyebrow gibbons. All of these fragile sites at the homologous regions of chromosomes are evolutionarily conserved and may be the indicators of common origin of chromosomes as genetic markers. Combined with G-banding patterns of chromosomes, the conservative fragile sites at homologous regions of chromosomes may be useful for the study of chromosome homology and evolution in closely-related animals. Out results show that the differences of chromosome 1 between Rhesus monkeys and human may involve a pericentric inversion and a paracentric inversion. However, the differences of chromosome 1 between Rhesus monkeys and White-eyebrow gibbons may caused by a pericentric inversion, a pericentric inversion, and a deletion or a translocation. The main differences of chromosome 3 between Rhesus monkeys and human may involve a pericentric inversion. Out results indicate that the differences of chromosome 7 between White-eyebrow gibbons and human may involve a paracentric inversion.(ABSTRACT TRUNCATED AT 250 WORDS)
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D000818:Animals; D005075:Biological Evolution; D002871:Chromosome Banding; D043283:Chromosome Fragile Sites; D002873:Chromosome Fragility; D005260:Female; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D006922:Hylobates / Q000235:genetics*; D008251:Macaca / Q000235:genetics*; D008253:Macaca mulatta / Q000235:genetics*; D008297:Male