title: [Chromosomal proteins in chick embryo erythrocytes on transcriptionally active and inactive genes].
abstract: Using chicken embryonic erythrocytes as a model, an experimental scheme for comparing the density of linker histones and high mobility group proteins on single-copy sequences of eukaryotic genome has been developed, thus permitting to probe alterations in the chromosomal protein pattern of transcribing chromatin. The report provides experimental evidence for validity of intracellular DNA-protein cross-linking, immunoaffinity chromatography and hybridization with single-stranded probes. Depletion of linker histones and enrichment of HMG 14/17 were shown to be the discriminating feature for transcriptionally active globin gene chromatin as opposed to inactive ovalbumin and lysozyme gene chromatin.
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Postnikov
authors.forename: V V
authors.initials: VV
authors.lastname: Shik
authors.forename: A V
authors.initials: AV
authors.lastname: Beliavskiĭ
authors.forename: K L
authors.initials: KL
authors.lastname: Brodolin
authors.forename: K R
authors.initials: KR
authors.lastname: Khrapko
authors.forename: T A
authors.initials: TA
authors.lastname: Nikol'skaia
authors.forename: A D
authors.initials: AD
authors.lastname: Mirzabekov
mesh_terms: D000818:Animals; D002642:Chick Embryo; D004247:DNA / Q000032:analysis / Q000235:genetics*; D015180:Electrophoresis, Gel, Two-Dimensional; D004912:Erythrocytes / Q000648:ultrastructure*; D005914:Globins / Q000235:genetics; D006609:High Mobility Group Proteins / Q000032:analysis / Q000235:genetics*; D006657:Histones / Q000032:analysis / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D014158:Transcription, Genetic
chemical_list: D006609:High Mobility Group Proteins; D006657:Histones; D005914:Globins; D004247:DNA
vernacular_title: Khromosomnye belki éritrotsitov émbrionov kur na transkriptsionno aktivnykh i neaktivnykh genakh.