Modulation of albumin secretion by ornithine alpha-ketoglutarate in adult rat hepatocyte cultures and a human hepatoma cell line (HepG2).
Cocultured adult rat hepatocytes and a human hepatoma cell line (HepG2) were maintained in an arginine-free medium with or without ornithine alpha-ketoglutarate. This drug increased greatly hepatocyte albumin secretion in both culture models. L-Ornithine was the component accounting for these effects since similar data were obtained by using this sole amino acid. Moreover, we observed that L-ornithine stimulation of albumin production was via polyamine synthesis. Since a correlated increase in albumin mRNA was observed, it may be postulated that ornithine alpha-ketoglutarate acts at a pretranslational level.
Lescoat
G
G
INSERM U 49, Unité de Recherche Hépatologique, Rennes, France.
Desvergne
B
B
Loreal
O
O
Pasdeloup
N
N
Deugnier
Y
Y
Bourel
M
M
Brissot
P
P
D000418:Albumins / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D006801:Humans; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology*; D009952:Ornithine / Q000031:analogs & derivatives* / Q000494:pharmacology; D051381:Rats
D000418:Albumins; C002118:ornithine alpha-ketoglutarate; D009952:Ornithine