Double tracer autoradiographic method for sequential evaluation of regional cerebral perfusion.
A new double tracer autoradiographic method for the sequential evaluation of altered regional cerebral perfusion in the same animal is presented. This method is based on the sequential injection of two tracers, 99mTc-hexamethylpropyleneamine oxime and N-isopropyl-(125I)p-iodoamphetamine. This method is validated in the assessment of brovincamine effects on regional cerebral perfusion in an experimental model of chronic brain ischemia in the rat. The drug enhanced perfusion recovery in low-flow areas, selectively in surrounding areas of infarction. The results suggest that this technique is of potential use in the study of neuropharmacological effects applied during the experiment.
Matsuda
H
H
Department of Nuclear Medicine, School of Medicine, Kanazawa University, Japan.
Tsuji
S
S
Oba
H
H
Kinuya
K
K
Terada
H
H
Sumiya
H
H
Shiba
K
K
Mori
H
H
Hisada
K
K
Maeda
T
T
D000662:Amphetamines; D000818:Animals; D001345:Autoradiography / Q000379:methods; D001921:Brain / Q000000981:diagnostic imaging*; D002545:Brain Ischemia / Q000000981:diagnostic imaging / Q000188:drug therapy; D002560:Cerebrovascular Circulation / Q000502:physiology*; D007457:Iodine Radioisotopes; D020367:Iofetamine; D008297:Male; D015609:Organotechnetium Compounds; D010091:Oximes; D011877:Radionuclide Imaging; D051381:Rats; D011919:Rats, Inbred Strains; D019690:Technetium Tc 99m Exametazime; D014665:Vasodilator Agents / Q000627:therapeutic use; D014749:Vincamine / Q000031:analogs & derivatives / Q000627:therapeutic use
D000662:Amphetamines; D007457:Iodine Radioisotopes; D015609:Organotechnetium Compounds; D010091:Oximes; D014665:Vasodilator Agents; D019690:Technetium Tc 99m Exametazime; D014749:Vincamine; C033581:11-bromovincamine; D020367:Iofetamine