Effect of epinephrine, propranolol, and verapamil on the chemiluminescence of neutrophils in duodenal ulcer patients.
To answer the question whether beta-adrenergic receptor agonists or antagonists and calcium channel blocking agents affect activation of neutrophils in vivo, the chemiluminescence (CL) test was employed. The intensity of emitted photons was amplified by luminol. The effect of investigated agents was measured after stimulation of isolated peripheral blood polymorphonuclear leukocytes (PMNLs) with opsonized zymosan particles. The investigations were performed on 9 duodenal ulcer patients, aged 19-23 years, after informed consent. Single subcutaneous dose of epinephrine (0.014 mg/kg) induced a marked increase in PMNL number and a moderate, but significant, decrease in CL 30 min after injection. Verapamil (0.15 mg/kg intravenously) diminished CL, propranolol (0.1 mg/kg intravenously) enhanced CL, but 150 min after injections CL was approaching the initial values. The obtained results suggest that the investigated compounds may modify the PMNL function in vivo.
Markiewicz
K
K
1st Department of Internal Medicine, Military Medical Academy, Lódz.
Kantorski
J
J
Malec
P
P
Tchórzewski
H
H
D000328:Adult; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D004381:Duodenal Ulcer / Q000378:metabolism*; D004837:Epinephrine / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D009504:Neutrophils / Q000166:cytology* / Q000187:drug effects; D011433:Propranolol / Q000494:pharmacology*; D014700:Verapamil / Q000494:pharmacology*
D011433:Propranolol; D014700:Verapamil; D004837:Epinephrine