Conformational characteristics of 4-acetylcytidine found in tRNA.
The conformational characteristics of a modified nucleoside 4-acetylcytidine (ac4C) was analyzed by proton nuclear magnetic resonance spectroscopy. This nucleoside was found to take the C3'-endo form predominantly. The enthalpy difference between the C2'-endo form and the C3'-endo form of the ribose moiety of ac4C was determined and 4-acetylation was found to stabilize the C3'-endo form further by 0.83 kcal.mol-1. This conformational characteristics of ac4C is probably important for the correct codon recognition and the stability of the tertiary structure of tRNA molecule.
Kawai
G
G
Faculty of Engineering, Yokohama National University, Japan.
Hashizume
T
T
Miyazawa
T
T
McCloskey
J A
JA
Yokoyama
S
S
D003562:Cytidine / Q000031:analogs & derivatives*; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D012343:RNA, Transfer
C017816:N-acetylcytidine; D003562:Cytidine; D012343:RNA, Transfer