[Effects of UV-irradiation of carp sperm and its modification by caffeine].
In connection with the development of UV-induced mutagenesis in carp, the effects of UV-irradiation of sperms have been studied in the range of doses 0.3-40 J/m2. The irradiation did not cause reduced fertilization ability and cleavage delay. Small doses of irradiation (0.6 J/m2) produced stimulating effect on embryo survival, the larvae yield decreasing and the amount of aberrant anaphases increasing, as the doses are increased. LD50 is 6.0 J/m2 for embryonal period of carp development, photoreactivation increases it up to 23.5 J/m2. Correlation between embryo viability and their resistance to UV has been found (r = +0.68 +/- 0.20). Modification of the effect of sperms irradiation with caffeine has been also studied. Strengthening of lethal and cytogenetic effects was only observed in case when embryos were placed in the caffeine solution, prior to onset of the first DNA replication in the male pronucleus. This may indicate the existence of caffeine-dependent prereplicative repair in carp embryos.
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D000818:Animals; D002110:Caffeine / Q000494:pharmacology*; D002347:Carps / Q000196:embryology / Q000235:genetics*; D003530:Cyprinidae / Q000235:genetics*; D008297:Male; D009154:Mutation; D013094:Spermatozoa / Q000187:drug effects / Q000528:radiation effects*; D014466:Ultraviolet Rays
D002110:Caffeine
Effekty UF-oblucheniia spermiev karpa i ikh modifikatsiia kofeinom.