title: Microtubules rich in modified alpha-tubulin characterize the tail processes of motile fibroblasts.
abstract: The organisation of microtubules rich in post-translationally modified alpha-tubulin has been investigated in a fibroblast cell line (NIH-3T3-T15) that can be reversibly transformed. An immunofluorescence microscopy study of the static non-transformed cells has revealed a central distribution of wavy microtubules showing post-translational modifications. When transformed there is a marked increase in cell motility and the appearance of long thin cytoplasmic 'tails'. These tails have been found to contain conspicuous bundles of post-translationally modified microtubules that run down the length of the processes and terminate close to the plasmalemma. Both detyrosinated and acetylated alpha-tubulin are present as major species in these modified microtubules. Such a pattern of modified microtubules is only occasionally seen in the untransformed NIH-3T3-T15 cells. We have also found them to be present in other transformed fibroblast lines. The presence of bundles of microtubules rich in modified alpha-tubulin in the cell tails is correlated with a marked reduction in the numbers of F-actin stress fibres. The possible role of these modified stable microtubules in cell motility is discussed.
authors.affiliation: School of Biological Sciences, University of East Anglia, Norwich, UK.
authors.forename: A R
authors.initials: AR
authors.lastname: Prescott
authors.forename: M
authors.initials: M
authors.lastname: Vestberg
authors.forename: R M
authors.initials: RM
authors.lastname: Warn
mesh_terms: D002460:Cell Line; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D008854:Microscopy, Electron; D008870:Microtubules / Q000648:ultrastructure*; D014404:Tubulin / Q000648:ultrastructure*
chemical_list: D014404:Tubulin