Methylamines and polyols in kidney, urinary bladder, urine, liver, brain, and plasma. An analysis using 1H nuclear magnetic resonance spectroscopy.
Methylamines and polyols are known to behave as organic osmolytes in the adaptation of many cells to hyperosmolar conditions. Using 1H nuclear magnetic resonance spectroscopy to analyze perchloric acid extracts we have examined several tissues in the rat for the presence of these compounds. Methylamines such as glycerophosphorylcholine, choline and betaine were observed in the renal inner medulla, urinary bladder, urine, liver, brain, and plasma. Myoinositol was relatively abundant in the renal inner medulla and brain whereas sorbitol was detected only in the inner medulla. A variety of unidentified compounds was also detected in each tissue. Although these methylamines and polyols are known to respond to osmotic changes in the renal inner medulla, their responses in other tissues remain to be investigated.
Gullans
S R
SR
Renal Division, Brigham and Women's Hospital, Boston, Mass.
Heilig
C W
CW
Stromski
M E
ME
Blumenfeld
J D
JD
D000818:Animals; D001622:Betaine / Q000097:blood / Q000378:metabolism / Q000652:urine; D001921:Brain / Q000378:metabolism; D002794:Choline / Q000097:blood / Q000378:metabolism / Q000652:urine; D005997:Glycerylphosphorylcholine / Q000097:blood / Q000378:metabolism / Q000652:urine; D007294:Inositol / Q000097:blood / Q000378:metabolism / Q000652:urine; D007668:Kidney / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008744:Methylamines / Q000097:blood / Q000378:metabolism* / Q000652:urine; D051381:Rats; D011919:Rats, Inbred Strains; D013012:Sorbitol / Q000097:blood / Q000378:metabolism / Q000652:urine; D013402:Sugar Alcohols / Q000378:metabolism*; D001743:Urinary Bladder / Q000378:metabolism*
D008744:Methylamines; D013402:Sugar Alcohols; D001622:Betaine; D007294:Inositol; D013012:Sorbitol; D005997:Glycerylphosphorylcholine; D002794:Choline