The protein kinase C inhibitor H-7 inhibits concanavalin A induced T-lymphocyte activation.
1. 1-(5-isoquinoline sulfonyl)-2-methylpiperazine (H-7), a protein kinase C inhibitor, was found to inhibit con A stimulated [3H]thymidine incorporation and cytosolic protein kinase C (PKC) activation in T-lymphocytes of mouse spleen. 2. The inhibitory effect of H-7 was both concentration and time-dependent. 3. H-7 exerted no inhibition when T-lymphocytes have been preincubated with con A for 10 hours or longer. 4. These results support the notion that PKC is an important element of the con A mitogenic signal transduction mechanism and the PKC signal is completed within the first 10 hr of con A incubation.
Xu
H
H
Chinese Medicinal Material Research Centre, Chinese University of Hong Kong, Shatin.
Fan
S G
SG
Lee
C M
CM
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000818:Animals; D003208:Concanavalin A / Q000037:antagonists & inhibitors; D066298:In Vitro Techniques; D007546:Isoquinolines / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008934:Mitogens; D010879:Piperazines / Q000494:pharmacology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors* / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000187:drug effects* / Q000201:enzymology; D013936:Thymidine / Q000378:metabolism
D007546:Isoquinolines; D008934:Mitogens; D010879:Piperazines; D003208:Concanavalin A; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D011493:Protein Kinase C; D013936:Thymidine