title: Effect of cycloheximide upon maturation of bovine oocytes.
abstract: Germinal vesicle breakdown (GVBD) of bovine oocytes was completely blocked by cycloheximide added to culture medium at concentrations of 1-20 micrograms/ml. Nevertheless, under such conditions a certain degree of chromatin condensation inside the germinal vesicle was observed. The inhibitory effect was not influenced by the presence or absence of cumulus cells and was fully reversible; but the process of GVBD was then significantly accelerated. The critical period in which the proteins necessary for GVBD are synthesized lasts approximately the first 5 h of culture. When germinal vesicle-arrested oocytes are fused to maturing bovine oocytes containing condensed chromosomes, GVBD of immature oocytes occurs within 3 h, even in the presence of cycloheximide. In the mouse, GVBD cannot be inhibited by protein synthesis inhibitors. When immature mouse oocytes are fused with immature bovine oocytes and the giant cells are then cultured in cycloheximide-supplemented medium, both GVs are observed, or only mouse GVBD occurs in common cytoplasm after 8 h of culture. We conclude that protein synthesis is necessary for GVBD of bovine oocytes. Our results also suggest that maturation-promoting factor (MPF) is not autocatalytically amplified in mammalian oocytes.
authors.affiliation: Institute of Animal Production, Prague, Czechoslovakia.
authors.forename: M
authors.initials: M
authors.lastname: Simon
authors.forename: F
authors.initials: F
authors.lastname: Jílek
authors.forename: J
authors.initials: J
authors.lastname: Fulka
mesh_terms: D000818:Animals; D002417:Cattle; D002459:Cell Fusion; D002478:Cells, Cultured; D002843:Chromatin / Q000648:ultrastructure; D003513:Cycloheximide / Q000494:pharmacology*; D005260:Female; D000090204:Mesothelin; D051379:Mice; D009865:Oocytes / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D014176:Protein Biosynthesis; D013045:Species Specificity
chemical_list: D002843:Chromatin; C000715528:Msln protein, mouse; D003513:Cycloheximide; D000090204:Mesothelin