The synthesis and characteristics of adenylyl-(5'----N epsilon)-lysyl peptides.
The dipeptides Boc-Thr-Lys(Cbz)-OMe and Boc-Lys(Cbz)-Glu(OEt)-OEt were prepared by the classical method of peptide synthesis. The Cbz protecting group was subsequently removed via hydrogenolysis. AMP was then covalently joined to the free lysine epsilon-amine by the carbodiimide (DCC) method. The resulting RNA-ligase active center model compounds, the adenylyl-(5----N epsilon)-lysylpeptides were then used to study the participation of the amino acid residue functional groups in the hydrolysis of the phosphoamide center.
Juodka
B
B
Department of Biochemistry and Biophysics, Vilnius University, Lithuania SSR, USSR.
Sasnauskiene
S
S
D000241:Adenosine; D004151:Dipeptides / Q000138:chemical synthesis*; D006868:Hydrolysis; D008956:Models, Chemical; D011130:RNA Ligase (ATP)
D004151:Dipeptides; D011130:RNA Ligase (ATP); D000241:Adenosine