title: Isolation and partial characterization of species-specific and cross-reactive antigens of Echinococcus granulosus cyst fluid.
abstract: Two parasite antigens have been isolated from Echinococcus granulosus hydatid cyst fluid using hydrophobic interaction chromatography, anion exchange chromatography and gel filtration chromatography. Initial characterization of the antigens indicates that both are glycoproteins, of approximately 20 and 48 kDa (Eg20 and Eg48). When the two antigens were tested with a battery of antisera from patients with heterologous parasitic infections, only Eg20 was found to be specific for E. granulosus. The Eg48 antigen cross-reacted with the sera of 33% of E. multilocularis patients. In both antigens, some of the epitopes recognized by antibodies in the sera of hydatid patients were periodate-sensitive. This suggests the involvement of carbohydrates in at least some of the antigenic determinants. Due to the abundance of the Eg48 antigen in the hydatid cyst fluid, it would be the more practically useful antigen for disease diagnosis, especially in countries where only E. granulosus is endemic.
authors.affiliation: Bernhard Nocht Institute for Tropical Medicine, Hamburg, F.R.G.
authors.forename: F M
authors.initials: FM
authors.lastname: al-Yaman
authors.forename: J
authors.initials: J
authors.lastname: Knobloch
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis*; D002845:Chromatography; D003429:Cross Reactions; D004443:Echinococcosis / Q000276:immunology*; D004446:Echinococcus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000032:analysis / Q000276:immunology*; D008970:Molecular Weight; D011402:Pronase; D012756:Sheep; D013045:Species Specificity
chemical_list: D000947:Antigens, Helminth; D006023:Glycoproteins; D011402:Pronase