Pan natural killer cell monoclonal antibodies and their relationship to the NK1.1 antigen.
The study of natural killer (NK) has been difficult because they account for a small percentage of peripheral blood and splenic lymphocytes and the paucity of NK specific antigens that have been identified. We have isolated pure populations of C57BL/6 (H-2b) NK cells using the IgG2b monoclonal antibody PK136 (anti-NK1.1). These NK1.1+ cells were used to immunize 129/J (H-2b) mice, and in this report, we describe three new NK specific monoclonal antibodies (SW3A4(IgM), SW4B12(IgG1), and SW2B4(IgG2b] and their relationship to the known murine NK antigen NK1.1. We have further characterized the NK1.1 antigen as a 39 kd molecule which is coded for by a gene which appears to map to chromosome 6.
Sentman
C L
CL
Department of Pathology and Immunology Graduate Program, University of Texas Southwestern Medical Center, Dallas.
Hackett
J
J
Moore
T A
TA
Tutt
M M
MM
Bennett
M
M
Kumar
V
V
D000818:Animals; D000911:Antibodies, Monoclonal; D000950:Antigens, Ly / Q000302:isolation & purification; D002469:Cell Separation; D007694:Killer Cells, Natural / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D013045:Species Specificity; D014407:Tumor Cells, Cultured / Q000276:immunology
D000911:Antibodies, Monoclonal; D000950:Antigens, Ly