Na+/H+ exchange in glial cells of Necturus optic nerve.
Single and double-barreled pH-sensitive electrodes were used to study intracellular pH (pHi) regulation in glial cells of Necturus optic nerve in the nominal absence of HCO3-/CO2. After the cells were acidified by the addition and withdrawal of NH4+, the pHi recovered toward the original steady-state pHi. The recovery from acidification was Na+-dependent and inhibited by 1 mM amiloride. These results suggest the existence in intact vertebrate glial cells of a Na+/H+ exchanger which functions in acid extrusion.
Astion
M L
ML
Institute of Neurobiology, University of Puerto Rico Medical Sciences, San Juan 00901.
Chvátal
A
A
Orkand
R K
RK
D000818:Animals; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008564:Membrane Potentials; D009337:Necturus / Q000378:metabolism* / Q000502:physiology; D009457:Neuroglia / Q000378:metabolism* / Q000502:physiology; D012964:Sodium / Q000378:metabolism*
D006859:Hydrogen; D012964:Sodium