Secretion by the mandibular gland of the red kangaroo (Macropus rufus) during isoprenaline infusion.
Intracarotid infusion of isoprenaline, either alone or in combination with acetylcholine infusion was used to stimulate salivation by the mandibular glands of anaesthetized red kangaroos. Isoprenaline alone (0.20-1.25 eta mol.kg-1.min-1) elicited flow rates ranging from 0.014 to 0.239 ml.min-1 (1.21-28.1 microliters.g gland-1.min-1). Salivary concentrations of sodium, chloride, phosphate and urea were negatively correlated with flow, whereas potassium, calcium, magnesium, hydrogen ion, bi-carbonate, protein, and osmolality were poorly correlated with flow. Relative to cholinergic saliva produced at equivalent flow rates, isoprenaline-evoked saliva had higher osmolality, saliva/plasma urea ratios and concentrations of protein, potassium, magnesium, bicarbonate, and phosphate, but lower sodium, chloride and hydrogen ion levels. At a steady salivary flow (0.5 ml.min-1), superim-position of isoprenaline infusion (0.15 eta mol.kg-1.min-1) on a pre-existing acetylcholine infusion reduced the rate of acetylcholine administration necessary to maintain flow, increased osmolality and the concentrations of protein, urea, potassium, calcium, magnesium, bicarbonate and phosphate and decreased sodium, chloride and hydrogen ion in the saliva. Salivary amylase activity was low and highly variable and the amylase activity/protein ratio fell substantially during isoprenaline stimulation. These results support the conclusion that the enzyme is of extrinsic origin. The response of the kangaroo mandibular gland to isoprenaline stimulation was very similar to that reported for rat mandibular gland, suggesting that the same ion transport phenomena underlie mandibular secretion in both species and probably in therian mammals generally.
Beal
A M
AM
School of Biological Science, University of N.S.W. Kensington, Australia.
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D002339:Carotid Arteries; D004573:Electrolytes / Q000097:blood; D005260:Female; D007269:Injections, Intra-Arterial; D007545:Isoproterenol / Q000494:pharmacology*; D007614:Macropodidae / Q000378:metabolism*; D008297:Male; D008334:Mandible; D008394:Marsupialia / Q000378:metabolism*; D012463:Saliva / Q000378:metabolism; D012469:Salivary Glands / Q000378:metabolism*; D014508:Urea / Q000097:blood
D004573:Electrolytes; D014508:Urea; D007545:Isoproterenol; D000109:Acetylcholine