title: Subcellular compartmentation of dolichol taken up by mouse leukemia cells.
abstract: [1-3H]Dolichol was intraperitoneally incubated with mouse leukemia Lcb 35 cells. The radioactive lipid taken up by the cells was subsequently recovered in two fractions separated by sucrose density gradient centrifugation: a particular, most probably, lysosomal fraction and a cytoplasmic one. Thin-layer chromatography of radioactive lipids present in both fractions revealed that majority of dolichol was unchanged and only a small part esterified with fatty acids. Distribution of dolichol in various mouse organs was examined. Of all the organs examined, spleen accumulated the highest amount of radioactivity.
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland.
authors.forename: W
authors.initials: W
authors.lastname: Jankowski
authors.forename: A
authors.initials: A
authors.lastname: Szkopińska
authors.forename: E
authors.initials: E
authors.lastname: Kula-Swiezewska
mesh_terms: D000818:Animals; D001202:Ascitic Fluid; D002451:Cell Compartmentation; D002499:Centrifugation, Density Gradient; D004286:Dolichols / Q000493:pharmacokinetics*; D007942:Leukemia, Experimental / Q000378:metabolism*; D008055:Lipids / Q000302:isolation & purification; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D014407:Tumor Cells, Cultured
chemical_list: D004286:Dolichols; D008055:Lipids