Internalization and limited processing of basic fibroblast growth factor on Chinese hamster lung fibroblasts.
Using either acidic (pH 2.5) or trypsic treatments, we demonstrated that 125I-labeled basic Fibroblast Growth Factor (125I-bFGF) was submitted to an internalization process on responsive Chinese hamster lung fibroblasts (CCL39) at 37 degrees C. Various experiments based on the measurement of cell-associated radioactivity, as well as on research of degradated products of 125I-bFGF in cellular supernatants, showed that most of the internalized radioactivity remained intracellularly located after up to 5 hr of incubation. Analyses of this radioactivity by NaDodSO4-PAGE revealed the presence of labeled peptides issued from the limited processing of the native 125I-bFGF form (17 kD) and whose molecular weights were estimated to be 9 and 6 kD. Kinetic experiments indicated that proteolysis of the 125I-bFGF began early on incubation (less than 30 min) and led to a prolonged preservation of the 9- and 6-kD peptides which were still detectable after 13 hr of incubation. Preincubation of the cells with different lysosomotropic agents completely inhibited the proteolysis, indicating that this event occurred probably in an intracellular acidic compartment. Two enzyme inhibitors, leupeptin and N-alpha-tosyl-L-lysine chloromethyl ketone (TLCK), were also shown to interfere with the formation of both 9- and 6-kD peptides, thus suggesting a way to control the appearance of these fragments, and hence to determine their potential intracellular role.
Moenner
M
M
Université Paris-Val de Marne, Laboratoire de biotechnologie des cellules eucaryotes, Créteil, France.
Gannoun-Zaki
L
L
Badet
J
J
Barritault
D
D
D000818:Animals; D001692:Biological Transport; D002451:Cell Compartmentation / Q000502:physiology; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004261:DNA Replication; D005346:Fibroblast Growth Factors / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism*; D007457:Iodine Radioisotopes; D008168:Lung / Q000166:cytology; D008970:Molecular Weight; D011480:Protease Inhibitors / Q000494:pharmacology; D011499:Protein Processing, Post-Translational; D013696:Temperature
D007457:Iodine Radioisotopes; D011480:Protease Inhibitors; D005346:Fibroblast Growth Factors