[Use of a microfluorometric method for measuring free calcium in the cytoplasm of isolated cultured rat cardiomyocytes].
Dual wavelength microfluorometry was used to measure the cytoplasmic free calcium concentration [( Ca2+]in) in single cultured cells from ventricular myocytes of neonatal rats loaded with the indicator fura-2. At 2.5 nmol/l extracellular Ca2+ in the resting cells [Ca2+]in was between 80 and 110 nmol/l. Sometimes, spontaneous low-frequency (approximately 0.1 Hz) [Ca2+]in oscillations were observed. High-potassium depolarization led to a Ca2+-antagonists-sensitive rise of [Ca2+]in. Both caffeine++ (5-10 mmol/l) and thymol (lmmol/l) initialized transient increase of [Ca2+]in. Mechanisms of [Ca2+]in homeostasis in heart muscle cells were discussed.
Belan
P V
PV
Verkhratskiĭ
A N
AN
Pronchuk
N F
NF
Tepikin
A V
AV
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism*; D005470:Fluorometry / Q000379:methods; D066298:In Vitro Techniques; D009206:Myocardium / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats
D002118:Calcium
Primenenie metoda mikrofliuorometrii dlia izmereniia svobodnogo ka'ltsiia v tsitoplazme odinochnykh ku'ltiviruemykh kardiomiotsitov krysy.