[The absence of DNA methylase activity in Drosophila cells].
DNA methylase activity has been studied in partially purified extracts from cultured cells, embryos, and adult Drosophila flies. No significant level of transfer of methyl groups from S-adenosylmethionine with formation of 5-methylcytosine and 6-methyladenine was observed. Methylase activity in Drosophila cells as compared to bovine lymphocytes and rat liver is either absent or at least 5000-15,000 times lower and hence cannot be detected using the present method.
Mazin
A L
AL
Gimadutdinov
O A
OA
Antoniv
T T
TT
Mukhovatova
L M
LM
Shuppe
N G
NG
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured / Q000201:enzymology; D015254:DNA Modification Methylases / Q000032:analysis* / Q000302:isolation & purification; D004330:Drosophila / Q000201:enzymology*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000201:enzymology / Q000662:veterinary; D008099:Liver / Q000201:enzymology; D008115:Liver Regeneration / Q000502:physiology; D008214:Lymphocytes / Q000201:enzymology; D008297:Male; D051381:Rats
D015254:DNA Modification Methylases
Otsutstvie DNK-metilaznoĭ aktivnosti v kletkakh drozofily.