[Changes in energy metabolism in the brain in experimental cerebral ischemia of different degree of severity (nuclear magnetic resonance-spectroscopic study)].
The peculiarities of brain energy metabolism were studied in male rats before and during cerebral ischemia of various severity elicited by bilateral common carotid arteries ligation. A multidimensional analysis was applied. In the rats which died after the ischemia, the NAD + NADH+/phosphocreatine (PCr) ratio and ATP content before ligation were higher than those in the surviving group. Also the strength of relationships between parameters of NMR spectra in each correlation matrix were 10 times higher and the variability of elements in each matrix was significantly lower in victims than those in the surviving group. The development of severe ischemia and the animals death were accompanied by an increase in the inorganic phosphate content, decrease in pH and stepwise disappearing of PCr and ATP. In animals surviving the same brain ischemia model, the changes in 31P spectra parameters pointed to some increase in the ratio of NAD + NADH+ only to ATP + ADP but not to PCr, and to an increase in summarized strength of correlation between 31P spectra parameters with the variability of elements decreased within each correlation matrix. Detection of these changes can be helpful in the diagnosis of mild ischemia without neurological deficit which already needs preventive therapy against more severe ischemia.
Gannushkina
I V
IV
Baranchikova
M V
MV
Semenova
N A
NA
Sibe'ldina
L A
LA
Likhodiĭ
S S
SS
Konradov
A A
AA
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002545:Brain Ischemia / Q000175:diagnosis / Q000378:metabolism*; D004734:Energy Metabolism; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D010761:Phosphorus Radioisotopes; D051381:Rats; D012720:Severity of Illness Index
D010761:Phosphorus Radioisotopes
Izmeneniia énergeticheskogo metabolizma mozga pri raznoĭ tiazhesti ego ishemii v éksperimente (IaMR-spektroskopicheskoe issledovanie).