A method for isolating and patch-clamping single mammalian taste receptor cells.
Individual taste receptor cells were isolated from the tongue of the mouse by enzymatic treatment followed by mechanical dissociation. The cells were morphologically identical with taste cells from amphibians. Whole-cell voltage-clamp recordings indicated that the murine taste cells possess a variety of voltage-dependent inward and outward currents. Delayed rectifier currents were blocked by denatonium benzoate, one of the most bitter compounds known. This preparation should permit a detailed electrophysiologcal investigation of taste transduction in mammals at the level of taste receptor cells.
Spielman
A I
AI
Monell Chemical Senses Center, School of Dental Medicine, Philadelphia, PA 19104.
Mody
I
I
Brand
J G
JG
Whitney
G
G
MacDonald
J F
JF
Salter
M W
MW
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D004558:Electric Stimulation; D008564:Membrane Potentials; D051379:Mice; D011984:Sensory Receptor Cells / Q000502:physiology*; D013649:Taste / Q000502:physiology*; D014059:Tongue / Q000166:cytology*