[Analysis of the distribution of low density lipoprotein flotation coefficients using an analytical centrifuge equipped with absorption optics].
Procedure of polydispersity determinations of low density lipoproteins (LDL) on low concentration scale (0.04%) using analytical ultracentrifuge with absorption optics was produced. No corrections for Johnston-Ogston effect and hydrostatic compressibility effect are required. Isothermal compressibility of LDL particles was estimated to be equal to 1.9 X 10(-5) Bar-1. An equation was obtained relating the flotation coefficients of LDL from different sources with solvent density and buoyant density of their particles. It was revealed that LDL particles from individual human plasma are divided into three-four subgroups having specific flotation characteristics and particular quantities of the material in these subgroups.
Morozkin
A D
AD
Medvedeva
N V
NV
Valentinova
N V
NV
D000042:Absorption; D001965:Bromides; D002499:Centrifugation, Density Gradient / Q000295:instrumentation*; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood*; D012964:Sodium; D017670:Sodium Compounds
D001965:Bromides; D008077:Lipoproteins, LDL; D017670:Sodium Compounds; D012964:Sodium; C027938:sodium bromide
Analiz raspredeleniia lipoproteinov nizkoĭ plotnosti po koéffitsientam flotatsii s pomoshch'iu analiticheskoĭ ul'tratsentrifugi, osnashchennoĭ absorbtsionnoĭ optikoĭ.