[Diagnosis of Legionella pneumonia by detection of antigenuria using an enzyme immunoassay with 6 antibody specificities].
In patients with microbiologically and clinically suspected Legionella caused pneumonia antigenuria was investigated by means of a direct two-site binding assay (ELISA) with polyclonal antibodies against Legionella (L.) pneumonia serogroup 1, 2, 3, 5 and 6 and L. micdadei. By application of antibodies only against L. pneumonia serogroup 1 antigenuria was found in 27 of 66 patients (= 41%). The expanding of the used specificities of antibodies in 47 out of this cases resulted in an increase of positive urinary antigen findings from 38% to 55%. Possibilities and limits of the detection of antigenuria with regard to efficient and rapid diagnostics of legionellosis are discussed.
Helbig
J H
JH
Lück
P C
PC
Witzleb
W
W
D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000652:urine*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007875:Legionella / Q000276:immunology*; D007877:Legionnaires' Disease / Q000175:diagnosis* / Q000276:immunology
D000942:Antigens, Bacterial
Diagnostik von Legionella-Pneumonien durch Nachweis der Antigenurie mittels Enzymimmunoassay unter Verwendung von 6 unterschiedlichen Antikörperspezifitäten.