title: Inverse relationship between renal and urinary kallikrein during chromate-induced acute renal failure in rat: urinary kallikrein excretion as a possible recovery index.
abstract: Acute renal failure (ARF) was induced in rat following a single injection of sodium chromate. A transient polyuria and a 10-fold decrease in glomerular filtration rate was immediately observed after sodium chromate administration. Urinary sodium and potassium excretion were reduced within 24 h and remained decreased for 8 to 10 days. Progressive recovery of normal renal functions, mainly electrolyte excretion and filtration rate was observed 12 days after sodium chromate administration. Urinary kallikrein excretion (UKE) was decreased only 48 h after sodium chromate administration. However the proportion of the active and inactive form excreted was unchanged. UKE remained also at a reduced level for 8 to 10 days and returned progressively to base-line level. The kallikrein content in the tissue was significantly increased immediately after sodium chromate administration and recovered normal values 12 days later. The increase of kallikrein in the tissue is more likely unspecific due to impaired protein transport than a specific stimulation of renal kallikrein biosynthesis. The decreased UKE may indicate a distal tubular reversible dysfunction in this ARF model. These reductions in electrolyte excretion, glomerular filtration and UKE were associated with selective morphological lesions. Whereas the glomeruli were intact, important damages affected proximal tubule cells which appeared necrotic and showed presence of vacuoles, liquefaction of cytoplasmic material and lost of microvilli. Less marked lesions were however observed in distal tubules, particularly large vacuoles were present at the apical poles of the tubule cells, the sites of kallikrein secretion. These distal damages may be involved in the increase of tissue concentration and in the decrease of UKE.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: INSERM (Institut National de la Santé et de la Recherche Médicale) Unité 133, Faculté de Médecine Rangueil, Toulouse, France.
authors.forename: J P
authors.initials: JP
authors.lastname: Girolami
authors.forename: C
authors.initials: C
authors.lastname: Orfila
authors.forename: C
authors.initials: C
authors.lastname: Pecher
authors.forename: G
authors.initials: G
authors.lastname: Cabos-Boutot
authors.forename: J L
authors.initials: JL
authors.lastname: Bascands
authors.forename: J P
authors.initials: JP
authors.lastname: Moatti
authors.forename: A
authors.initials: A
authors.lastname: Adam
authors.forename: A
authors.initials: A
authors.lastname: Colle
mesh_terms: D058186:Acute Kidney Injury / Q000139:chemically induced / Q000378:metabolism*; D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D002840:Chromates / Q000633:toxicity; D007610:Kallikreins / Q000378:metabolism* / Q000652:urine; D007684:Kidney Tubules / Q000378:metabolism*; D007686:Kidney Tubules, Distal / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D008657:Metabolic Clearance Rate; D008928:Mitochondria / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values
chemical_list: D002840:Chromates; D007610:Kallikreins