[Detection of mycoplasmas in experimentally-infected bull sperm].
Bull sperm pellets experimentally infected with various concentrations of Mycoplasma (M.) bovis, M. bovigenitalium, and M. californicum were stored several months at -196 degrees C and were subsequently tested for mycoplasmas, using different methods and culturing media. M. bovis was reliably identified by laboratory diagnosis up to a concentration of 10(2) cfu/pellet. M. bovigenitalium and M. californicum were found to make higher demands on the quality of culturing substrates. Recommended are 1-h broth incubation and 5-d broth breeding.
Richter
A
A
Pfützner
H
H
D000818:Animals; D002417:Cattle; D015925:Cryopreservation / Q000662:veterinary; D008297:Male; D009174:Mycoplasma / Q000302:isolation & purification*; D013094:Spermatozoa / Q000382:microbiology*
Nachweis von Mykoplasmen in experimentell infiziertem Bullensperma.