title: High performance liquid chromatographic (HPLC) determination of centchroman in human serum and application to single-dose pharmacokinetics.
abstract: A simple and sensitive (2 ng/ml) HPLC method with fluorescence detection has been developed to measure serum concentrations of centchroman, a new nonsteroidal antifertility agent. The method was sufficiently sensitive to follow the drug over 21 days in human volunteers. Pharmacokinetic parameters of centchroman were determined after a single oral dose of 60 mg (2 x 30-mg tablets) in two healthy female volunteers. Centchroman is slowly eliminated from serum, showing a biexponential disappearance curve from serum. The terminal half-life of centchroman in the two volunteers was 168 and 175 hr, respectively.
authors.affiliation: Pharmacokinetics and Metabolism Division, Central Drug Research Institute, Lucknow, India.
authors.forename: J K
authors.initials: JK
authors.lastname: Paliwal
authors.forename: R C
authors.initials: RC
authors.lastname: Gupta
authors.forename: P K
authors.initials: PK
authors.lastname: Grover
authors.forename: O P
authors.initials: OP
authors.lastname: Asthana
authors.forename: J S
authors.initials: JS
authors.lastname: Srivastava
authors.forename: S
authors.initials: S
authors.lastname: NityaNand
mesh_terms: D000328:Adult; D001578:Benzopyrans / Q000097:blood*; D002486:Centchroman / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006207:Half-Life; D006801:Humans; D015203:Reproducibility of Results
chemical_list: D001578:Benzopyrans; D002486:Centchroman