Calibration of 125I-polymer standards with 125I-brain paste standards for use in quantitative receptor autoradiography.
125I-Polymer standards were calibrated by interpolation of their optical densities in [125I]-brain paste standard curves to obtain dpm/mg protein. There was a linear relationship between the calibrated polymer standards and the dpm/mg polymer, as provided by the manufacturer. One dpm/mg polymer was equivalent to 7.34 +/- 0.22 dpm/mg protein. Receptor quantification in selected rat brain areas with comparison to either brain paste or calibrated polymer standards yielded similar results.
Nazarali
A J
AJ
Section on Pharmacology, National Institute of Mental Health, Bethesda, MD 20892.
Gutkind
J S
JS
Saavedra
J M
JM
D000818:Animals; D001345:Autoradiography / Q000592:standards*; D001921:Brain / Q000378:metabolism*; D007457:Iodine Radioisotopes / Q000592:standards*; D011108:Polymers / Q000592:standards*; D051381:Rats
D007457:Iodine Radioisotopes; D011108:Polymers