Probing the anticodon loop structure in yeast tRNA(Phe-Y) with single strand-specific nuclease S1.
Yeast tRNA(Phe) and tRNA(Phe-Y) are cleaved by single strand-specific endonuclease S1 at the same positions within the anticodon loop (phosphates 34, 36 and 37) and at the 3'-terminus (phosphates 75 and 76). The efficiency of the anticodon loop hydrolysis is much higher in tRNA(Phe-Y) while the cutting at the 3'-terminus is not influenced considerably by the Y-base1 removal from yeast tRNA(Phe). The effect of the Y-base excision on the structure of the anticodon loop is discussed on the basis of the S1 digestion studies as well as other relevant results.
Marciniec
T
T
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, Poland.
Ciesiołka
J
J
Krzyzosiak
W
W
D000926:Anticodon; D001230:Aspergillus / Q000235:genetics*; D001236:Aspergillus oryzae / Q000201:enzymology / Q000235:genetics*; D006868:Hydrolysis; D009690:Nucleic Acid Conformation; D015347:RNA Probes; D012345:RNA, Transfer, Amino Acid-Specific; D012360:RNA, Transfer, Phe; D015719:Single-Strand Specific DNA and RNA Endonucleases
D000926:Anticodon; D015347:RNA Probes; D012345:RNA, Transfer, Amino Acid-Specific; D012360:RNA, Transfer, Phe; D015719:Single-Strand Specific DNA and RNA Endonucleases