The synthesis of an active derivative of cyclomaltoheptaose for the hydrolysis of esters and the formation of amide bonds.
The synthesis is described of a derivative of cyclomaltoheptaose (beta-cyclodextrin) to which the tripeptide Ser-His-Asp, the catalytic triad found in chymotrypsin, has been coupled. The derivative enhanced the rates of hydrolysis of activated esters, as measured by the release of p-nitrophenol, and the formation of amine bonds.
Ekberg
B
B
Department of Pure and Applied Biochemistry, University of Lund, Sweden.
Andersson
L I
LI
Mosbach
K
K
D000577:Amides / Q000378:metabolism*; D000595:Amino Acid Sequence; D002264:Carboxylic Acids / Q000378:metabolism*; D055598:Chemical Phenomena; D002621:Chemistry; D002918:Chymotrypsin / Q000378:metabolism; D003505:Cyclodextrins / Q000138:chemical synthesis* / Q000378:metabolism; D003912:Dextrins / Q000138:chemical synthesis*; D004952:Esters / Q000378:metabolism*; D006868:Hydrolysis; D009596:Nitrophenols; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000378:metabolism; D013213:Starch / Q000138:chemical synthesis*; D047392:beta-Cyclodextrins
D000577:Amides; D002264:Carboxylic Acids; D003505:Cyclodextrins; D003912:Dextrins; D004952:Esters; D009596:Nitrophenols; D009842:Oligopeptides; D047392:beta-Cyclodextrins; D013213:Starch; D002918:Chymotrypsin; C031215:betadex; C024836:4-nitrophenol