[Mechanism of the protective action of kopsinine against hepatotoxicity of carbon tetrachloride].
Kopsinine 1 mmol/L inhibited microsomal lipid peroxidation (MDA) formation induced by CCl4 in vitro. Metyrapone, a specific inhibitor of cytochrome P-450, partially counteracted the inhibitory effect of kopsinine on MDA formation. Oral administration of kopsinine 200 mg/kg decreased the formation of diene conjugates in liver microsomes in CCl4 (1 ml/kg ig) intoxicated mice. Kopsinine also inhibited 14CCl4 covalent binding to lipids and proteins of liver microsomes. But the degree of inhibition was not as pronounced as that of inhibition of MDA. The metabolic conversion of CCl4 to carbon monoxide and the utilization of NADPH in the course of CCl4 metabolism by liver microsomes were increased by kopsinine in intro. It appears that to maintain the stability of membranes of liver cells is the mode of the protective action of kopsinine.
Huang
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Liu
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D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D002252:Carbon Tetrachloride Poisoning / Q000378:metabolism / Q000517:prevention & control*; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism / Q000517:prevention & control*; D026121:Indole Alkaloids; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D008862:Microsomes, Liver / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D000470:Alkaloids; D026121:Indole Alkaloids; D008315:Malondialdehyde; C061491:kopsinine