A new peptide for human parvovirus B19 antibody detection.
A serological assay for human parvovirus B19 was developed. Linear overlapping synthetic peptides were synthesised according to parts of open reading frames 1 and 2. A region at the N-terminus of viral protein VP2 detected serological reactivity in indirect enzyme-linked immunosorbent assays for IgG and IgM with known seropositive human sera. A cyclized peptide taken from this region, amino acids 284-307, gave the best selective reactivity with seropositive and seronegative sera. The peptide assay appears suitable for further studies of B19 infections and their complications.
Fridell
E
E
Department of Virology, National Bacteriological Laboratory, Stockholm, Sweden.
Trojnar
J
J
Wahren
B
B
D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D000907:Antibodies, Bacterial / Q000032:analysis*; D002213:Capsid / Q000276:immunology*; D036022:Capsid Proteins; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D004890:Erythema / Q000175:diagnosis; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D010321:Parvoviridae / Q000276:immunology*; D015995:Prevalence
D000907:Antibodies, Bacterial; D036022:Capsid Proteins