[Detection of Chlamydia trachomatis antigen for diagnosis of respiratory infection I. Cross reaction with bacteriae consisting normal flora respiratory tract].
We examined the cross reaction of Chlamydia trachomatis (C. trachomatis) by antigen detection method using EIA against the aerobic bacteria which colonize in the respiratory tract of children. Chlamydiazyme showed a cross reaction among 5 out of 7 species of Gram negative bacteria (= 71.4%) and Neisseria and Branhamella catarrhalis showed a cross reaction even in low concentrations of 1 X 10(3-4) CFU/ml. There were no Gram positive bacteria which showed cross reaction. IDEIA Mark III did not react to Gram negative bacteria even in high concentrations of 1 X 10(7-10) CFU/ml. One strain of coagulase positive staphylococcus (CPS) showed positive at a concentration of 1 X 10(8): however, other Gram positive bacteria including two other strains of CPS exhibited no cross reaction. The detectable concentration of C. trachomatis (D/UW 3 strain) using IDEIA Mark III was 1 X 10(3) IFU/ml. Low cross reaction rate and sensitivity suggest IDEIA Mark III is preferable for diagnosis of respiratory infection of C. trachomatis though further clinical studies are necessary.
Yamazaki
T
T
Hashizume
S
S
Yoshizawa
H
H
Yamazaki
T
T
D000942:Antigens, Bacterial / Q000032:analysis*; D002690:Chlamydia Infections / Q000175:diagnosis*; D002692:Chlamydia trachomatis / Q000276:immunology; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006090:Gram-Negative Bacteria / Q000276:immunology; D006801:Humans; D011933:Reagent Kits, Diagnostic; D012137:Respiratory System / Q000382:microbiology; D012141:Respiratory Tract Infections / Q000175:diagnosis*
D000942:Antigens, Bacterial; D011933:Reagent Kits, Diagnostic