The effect of dietary fat content and composition on adipocyte lipids in normal and diabetic states.
Cellular insulin resistance is a common feature of the diabetic and obese state. To determine the effect of dietary fat and the insulin resistant state of diabetes on adipose tissue composition, control and streptozotocin-induced diabetic rats were fed four diets differing in fat content (10 percent w/w or 20% w/w) and polyunsaturated to saturated fatty acid (P/S) ratios (0.2 or 2.0) for 6 weeks. At 3 weeks diabetes was induced in half the animals in each diet group. Increasing the fat content and P/S ratio of the diet increased the content of polyunsaturated fatty acids and decreased the contents of monounsaturated and omega-3 fatty acids. The higher level of C-18:2(6) and the lower levels of C20:4(6) and monounsaturated fatty acids observed in diabetic animals is consistent with altered desaturase enzyme activity. Diet and diabetes induced compositional changes in essential and non-essential fatty acids in the adipose tissue may alter the total body pools of available fatty acids for the synthesis of other lipids such as phospholipids.
Field
C J
CJ
Department of Foods & Nutrition, University of Alberta, Edmonton, Canada.
Goruk
S D
SD
Wierzbicki
A A
AA
Clandinin
M T
MT
D000273:Adipose Tissue / Q000032:analysis*; D000704:Analysis of Variance; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D004041:Dietary Fats / Q000494:pharmacology*; D005229:Fatty Acids, Monounsaturated / Q000032:analysis; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D008055:Lipids / Q000032:analysis*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D004041:Dietary Fats; D005229:Fatty Acids, Monounsaturated; D005231:Fatty Acids, Unsaturated; D008055:Lipids