Study on the in vitro assay method for evaluating the inhibitory effect of various substances on the production of plasma kallikrein.
The assay method based on the principle of kallikrein-kinin cascade was established for evaluating the inhibitory effects of various substances on the production of plasma kallikrein. In this in vitro assay, it was found that indomethacin, ketoprofen, ibuprofen and an extract obtained from inflamed rabbit skin inoculated with vaccinia virus (NSP) had the inhibitory effect on the production of plasma kallikrein. Kinins generated in the reaction mixture were measured by RIA. It was shown that the generation of kinins was also inhibited by these substances. From these results, it is hoped that this assay method may be useful for screening the substances which inhibited the production of kinin.
Nishikawa
K
K
Institute of Bio-Active Science, Nippon Zoki Pharmaceutical Co. Ltd., Hyogo, Japan.
Kawakubo
H
H
Matsumoto
K
K
Yago
H
H
Toyomaki
Y
Y
Suehiro
S
S
D000632:Aminopyrine / Q000494:pharmacology; D001241:Aspirin / Q000494:pharmacology; D006801:Humans; D007052:Ibuprofen / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology; D007610:Kallikreins / Q000097:blood*; D007660:Ketoprofen / Q000494:pharmacology; D007700:Kinetics; D007705:Kinins / Q000302:isolation & purification; D009020:Morphine / Q000494:pharmacology; D010423:Pentazocine / Q000494:pharmacology; D011288:Prekallikrein / Q000502:physiology*
D007705:Kinins; D000632:Aminopyrine; D009020:Morphine; D011288:Prekallikrein; D007660:Ketoprofen; D007610:Kallikreins; D001241:Aspirin; D010423:Pentazocine; D007052:Ibuprofen; D007213:Indomethacin