Radioimmunoassay of plasma 21-deoxycortisol.
A reliable radioimmunoassay for the determination of plasma 21-deoxycortisol (21-DF) after chromatography on Sephadex LH20 columns of methylene chloride plasma extracts has been described and evaluated. The antiserum used was raised in rabbits injected with 21-DF-3-(O-carboxy-methyl)oxime-bovine serum albumin. In men (n = 10) the levels ranged from 0.11 to 0.29 ng/ml (mean +/- SD: 0.21 +/- 0.06). In women the mean levels were in the follicular phase: 0.16 +/- 0.06 (range: 0.05-0.22; n = 10) and in the luteal phase: 0.18 +/- 0.06 (range: 0.10-0.35; n = 12). No cyclical change and no significant difference between male and female groups were observed. In patients with congenital adrenal hyperplasia due to 21-hydroxylase deficiency very high levels were observed. The higher concentration of 21-DF found in adrenal effluent than in peripheral plasma has provided direct evidence of its adrenal origin in patients without 21-hydroxylase deficiency.
Nahoul
K
K
Fondation de Recherche en Hormonologie, Fresnes, France.
Adeline
J
J
Bercovici
J P
JP
D015065:17-Hydroxycorticosteroids / Q000097:blood*; D000293:Adolescent; D000328:Adult; D003350:Cortodoxone / Q000097:blood*; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D006908:Hydroxyprogesterones / Q000097:blood; D007536:Isomerism; D008297:Male; D008875:Middle Aged; D011863:Radioimmunoassay
D015065:17-Hydroxycorticosteroids; D006908:Hydroxyprogesterones; C003556:21-deoxycortisol; D003350:Cortodoxone; D006854:Hydrocortisone