[Transport of adenosine across the endothelium of the isolated perfused rat aorta].
In order to define the role of vascular endothelium in the transport and metabolism of adenosine, endothelium-intact and denuded isolated rat aortas were perfused with 3H-adenosine. In the physiological concentration range of adenosine (0.01-1 microM) sequestration of adenosine by endothelium was 90-92%. Similar findings were obtained for inosine. Transfer of radioactive purines from the prelabeled endothelium to the underlying smooth muscle was rather small (1% per hour) and most likely proceeds via dephosphorylated purine compounds. Under steady state conditions the transendothelial adenosine gradient is only moderate because underlying vascular smooth muscle metabolize adenosine less rapidly.
Schrader
J
J
Physiologisches Institut I, Universität Düsseldorf, FRG.
Kroll
K
K
Kelm
M
M
Bürrig
K F
KF
D000241:Adenosine / Q000378:metabolism*; D000818:Animals; D001011:Aorta; D001693:Biological Transport, Active / Q000502:physiology; D046508:Culture Techniques; D004730:Endothelium, Vascular / Q000502:physiology*; D007288:Inosine / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000502:physiology*; D010477:Perfusion; D051381:Rats
D007288:Inosine; D000241:Adenosine
Transport von Adenosin über das Endothel der isoliertperfundierten Rattenaorta.