title: Neural differentiation of Xenopus laevis ectoderm takes place after disaggregation and delayed reaggregation without inducer.
abstract: When Xenopus blastula or early gastrula ectoderm is disaggregated and cells are kept dispersed for up to 5 h prior to reaggregation, the resulting spheres will differentiate into large neural structures. In contrast, dissociated and immediately reaggregated ectoderm will only differentiate into ciliated epidermis (so-called 'atypical epidermis'). Ectoderm treated with mesoderm-inducing XTC-conditioned medium during the period of reaggregation immediately after disaggregation will only form one- or two-cell types (notochord and somites) only. Ectoderm treated with XTC-factor prior to disaggregation will differentiate into a large variety of cell types.
authors.affiliation: Department of Zoophysiology, Universität GHS Essen, F.R.G.
authors.forename: H
authors.initials: H
authors.lastname: Grunz
authors.forename: L
authors.initials: L
authors.lastname: Tacke
mesh_terms: D000818:Animals; D002449:Cell Aggregation / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D004475:Ectoderm / Q000166:cytology* / Q000187:drug effects; D005260:Female; D008648:Mesoderm / Q000502:physiology; D009420:Nervous System / Q000166:cytology / Q000187:drug effects / Q000196:embryology; D009474:Neurons / Q000166:cytology* / Q000187:drug effects; D014982:Xenopus laevis / Q000196:embryology*