Activation of antitumor cytotoxicity of human blood mononuclear cells by a basic factor from dialysable human-leukocyte extract.
Dialysable human leukocyte extract (10 kDa molecular weight cutoff) contained a basic factor stimulating natural killer (NK) cytotoxicity of human peripheral blood mononuclear cells (PBMC) against human K562 tumor cells when PBMC were pre-incubated with the factor for 72h prior to cytotoxicity assays. This cytotoxicity-stimulating factor (CySF-L2) could be enriched by adsorption to ion exchange resin Dowex 50WX2 (H-form) eluting at pH 9.0-9.6 when a pH gradient between pH 3 and pH 10 was used. Further purification was achieved by chromatography on DEAE-Sepharose. The factor has a molecular weight of approximately 1000 Da and is insensitive to protease and exopeptidase treatment but sensitive against treatment with endoglycosidase F. Using immunomagnetic cell sorting, complement-mediated cell depletion and depletion by planning, the cytotoxic effector cells activated during pre-incubation of PBMC with CySF-L2 could be identified as CD16+ CD14+ monocytes/macrophages and as Leu7+ Leu19+ CD16+ CD3- CD8- NK cells.
Doelker
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I
Friedrich-Miescher-Laboratorium der Max-Planck-Gesellschaft Tuebingen, F.R.G.
Voetsch
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W
Anderer
F A
FA
D000328:Adult; D000367:Age Factors; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D005260:Female; D006801:Humans; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology*; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology*; D012016:Reference Values; D012737:Sex Factors; D014020:Tissue Extracts / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000276:immunology
D014020:Tissue Extracts