The production of highly specific anti-testosterone antisera using acid-treated bacteria as immunogenic carrier.
A novel and effective procedure for the production of highly specific anti-testosterone antibodies is described. It involved pretreatment of experimental animals with tolerogens followed by immunization with conjugates of testosterone covalently linked to acid-treated Salmonella minnesota R595 bacteria as immunogenic carriers. Antibodies elicited by this procedure showed minimal cross-reactivity towards 5 alpha-dihydrotestosterone and some of them were successfully used in radioimmunoassays for the determination of serum testosterone levels, even without the need for prior extraction.
Cilliers
J M
JM
Department of Biochemistry, University of Stellenbosch, Republic of South Africa.
Stindt
H W
HW
Bellstedt
D U
DU
D000085:Acetates; D000818:Animals; D003429:Cross Reactions; D013196:Dihydrotestosterone / Q000097:blood; D007106:Immune Sera; D007114:Immunization / Q000379:methods*; D011817:Rabbits; D011863:Radioimmunoassay; D012475:Salmonella / Q000276:immunology*; D013739:Testosterone / Q000097:blood / Q000276:immunology*
D000085:Acetates; D007106:Immune Sera; D013196:Dihydrotestosterone; D013739:Testosterone