Lonidamine effect on male rat germ cells.
Lonidamine, a dichlorinated derivative of indazole-3-carboxylic acid, has recently been indicated as an antiproliferative agent being able to reduce mitotic activity of tumor cells. We have evaluated lonidamine effect on proliferating, non tumor cells choosing as a model the male germ cells obtained from cultured seminiferous epithelium explants. The obtained germ cells are able to duplicate in vitro and we have found that lonidamine, at low doses, induces a significative inhibition of the incorporation of labelled thymidine into the duplicating germ cells. The effect seems to be specific for the germ cells since lonidamine does not affect duplicative ability of the somatic cells of the seminiferous tubules and of muscle fibroblasts.
Galdieri
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D000818:Animals; D000988:Antispermatogenic Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D007191:Indazoles / Q000494:pharmacology*; D008297:Male; D011720:Pyrazoles / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013094:Spermatozoa / Q000187:drug effects*; D013936:Thymidine / Q000378:metabolism
D000988:Antispermatogenic Agents; D007191:Indazoles; D011720:Pyrazoles; C016371:lonidamine; D013936:Thymidine