Distribution of ciliatine (2-aminoethylphosphonic acid) and phosphonoalanine (2-amino-3-phosphonopropionic acid) in human tissues.
Ciliatine (2-aminoethylphosphonic acid) was detected in the human brain, heart, kidney, liver, intestine, spleen, adrenal glands, and aorta. Phosphonoalanine (2-amino-3-phosphonopropionic acid) was found in the human liver, intestine and spleen. Tissue homogenates were extracted with trichloroacetic acid and a chloroform-methanol mixture. After hydrolysis, each fraction was subfractionated by ion-exchange chromatography and examined by paper chromatography and electrophoresis using a specific ninhydrin-molybdate staining procedure to detect the phosphonic acids. The acids were found bound either to lipid or to protein; no free phosphonic acid was detected.
Tan
S A
SA
Department of Medicine, Loma Linda University, Calif.
Tan
L G
LG
D000311:Adrenal Glands / Q000032:analysis; D000328:Adult; D000368:Aged; D000409:Alanine / Q000031:analogs & derivatives* / Q000032:analysis; D000615:Aminoethylphosphonic Acid / Q000032:analysis*; D001011:Aorta / Q000032:analysis; D001923:Brain Chemistry; D002845:Chromatography; D005260:Female; D006801:Humans; D007422:Intestines / Q000032:analysis; D007668:Kidney / Q000032:analysis; D008099:Liver / Q000032:analysis; D008297:Male; D009206:Myocardium / Q000032:analysis; D009943:Organophosphorus Compounds / Q000032:analysis*; D013154:Spleen / Q000032:analysis; D014018:Tissue Distribution
D009943:Organophosphorus Compounds; C000211:2-amino-3-phosphonopropionic acid; D000615:Aminoethylphosphonic Acid; D000409:Alanine