title: Chromosomal mapping and nucleotide sequence of a human DNA autonomously replicating sequence.
abstract: A 1.1-kb human DNA fragment (ARSH1) capable of functioning as a putative origin of replication in yeast cells has been characterized both by in situ hybridization to human metaphase chromosomes and by DNA sequencing. Our hybridization studies show a preferential localization of ARSH1 in chromosome regions 1p34-36 and 2q34-37. DNA sequence analysis indicates that in addition to the consensus sequence required for ARS function in yeast cells, nuclear matrix-associated DNA motifs are also present in the 1.1-kb fragment. These results suggest that ARSH1 sequences may serve as points of anchorage to the nuclear matrix for chromosomes 1 and 2.
authors.affiliation: Division of Biomedical Sciences, Meharry Medical College, Nashville, Tennessee 37208.
authors.forename: M
authors.initials: M
authors.lastname: Aguinaga
authors.forename: C
authors.initials: C
authors.lastname: Sharan
authors.forename: D N
authors.initials: DN
authors.lastname: Singh
authors.forename: D
authors.initials: D
authors.lastname: Kegler
authors.forename: K A
authors.initials: KA
authors.lastname: Siddiqui
authors.forename: M S
authors.initials: MS
authors.lastname: Valenzuela
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D002889:Chromosomes, Human, Pair 2; D004247:DNA / Q000235:genetics*; D004261:DNA Replication; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D015003:Yeasts / Q000235:genetics
chemical_list: D004247:DNA