title: Comparative study of methods of detection of hepatitis 'B' surface antigen (HBsAg).
abstract: The serum samples were collected from 52 patients of acute viral hepatitis and 235 hospital staff from Kasturba Hospital for Infectious Diseases. HBsAg was detected in their sera by counter-immuno-electrophoresis (CIEP), reverse passive hemogglutination (RPHA) and by micro-enzyme-linked-immunosorbent assay (ELISA) technique. Among the patients, HBsAg was detected in 12 cases (23%) by CIEP, in 18 cases (34%) by RPHA and in 23 patients (45%) by ELISA. In the hospital staff, HBsAg was detected in 4 samples (1.7%) by CIEP, in 8 samples (3.5%) by RPHA and in 32 samples (13.5%) by ELISA. Thus ELISA was found to be the most sensitive technique in detecting HBsAg.
authors.forename: V V
authors.initials: VV
authors.lastname: Parab
authors.forename: N N
authors.initials: NN
authors.lastname: Talawadekar
authors.forename: V V
authors.initials: VV
authors.lastname: Kale
authors.forename: S A
authors.initials: SA
authors.lastname: Kamat
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D005260:Female; D006514:Hepatitis B Surface Antigens / Q000032:analysis / Q000302:isolation & purification*; D006525:Hepatitis, Viral, Human / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged
chemical_list: D006514:Hepatitis B Surface Antigens