The effect of cationic electrolytes on the electrostatic behavior of cellular surfaces with ionizable groups.
Based on the assumption that the electrostatic charges on the surface of sheep leukocytes arise from the dissociation of ionogenic groups, together with the presence of divalent cation (or trivalent cation) in the suspending medium of low ionic strength (or high ionic strength), the non-linear Poisson-Boltzmann equation for cell interaction with a solid surface with constant potential (or constant charge) is numerically solved in this paper. The cellular surface potential and the repulsive (or attractive) force is expressed as the function of separation distance. Because of shrinking the thickness of the electrostatic double layer at high ionic strength, the presence of cationic electrolyte has a less influential role on both the cellular surface potential and interaction force than at low ionic strength. However, due to the continuous equilibration of the ionogenic groups on the cellular surface as separation distance decreases, the presence of cationic electrolyte will not always reduce the interaction force during the whole adhesion period. The distance at which the cationic electrolyte changes its effect from positive to negative is termed the critical separation distance in this paper.
Chang
Y I
YI
Department of Chemical Engineering, Tunghai University, Taichung, Taiwan.
D000818:Animals; D002412:Cations; D002448:Cell Adhesion / Q000502:physiology; D002462:Cell Membrane / Q000502:physiology*; D007962:Leukocytes / Q000648:ultrastructure; D008564:Membrane Potentials / Q000502:physiology; D008954:Models, Biological; D012756:Sheep
D002412:Cations