title: Covalent binding of 17 beta-estradiol and retinoic acid to proteins in the human breast cancer cell line MCF-7.
abstract: Both retinoic acid and 17 beta-estradiol formed covalent bonds with proteins of the human breast cancer cell line MCF-7. Two-dimensional gel patterns of the labeled proteins were unique for each ligand. There were four major retinoylated proteins in MCF-7 consisting of two doublets with molecular masses of 37 kDa and 20 kDa. These proteins were designated 37a, 37b, 20c, and 20d. The extent of retinoylation was very low in a 55 kDa protein that we previously identified in the human myeloid leukemia cell line HL60 [Takahashi, N. and Breitman, T. R. (1989) J. Biol. Chem. 264, 5159-5163]. These results indicated that the protein substrates for retinoylation may vary among cell-types. About 10 proteins were labeled from 17 beta-estradiol. Two of these proteins had mobilities that were identical to the retinoylated proteins 37a and 20c. These results indicate that in MCF-7 cells there are two proteins that can be retinoylated and labeled from estradiol. The demonstration that some ligands of the steroid/thyroid receptor family are covalently linked to cellular proteins suggests new mechanisms for the many effects of these agents on cells.
authors.affiliation: Division of Cancer Treatment, National Cancer Institute, Bethesda, Maryland 20892.
authors.forename: N
authors.initials: N
authors.lastname: Takahashi
authors.forename: T R
authors.initials: TR
authors.lastname: Breitman
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D004958:Estradiol / Q000378:metabolism*; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism; D014212:Tretinoin / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D009363:Neoplasm Proteins; D004958:Estradiol; D014212:Tretinoin