title: Resident macrophages specifically inhibit DNA synthesis in the nuclei of transformed cells in heterokaryons.
abstract: DNA synthesis in heterokaryons of mouse peritoneal macrophages and various proliferating cultured cells was analyzed. Macrophages did not inhibit replication in the nuclei of non-immortalized and spontaneously immortalized cells (rat fibroblasts and macrophages, mouse pre-macrophages, NIH3T3 cells). On the contrary, the percentage of DNA-synthesizing nuclei of malignant HeLa cells was drastically reduced in heterokaryons. The transformation of NIH3T3 cells with c-Ki-ras oncogene and that of rat chondrocytes with p53 oncogene made these cells sensitive to the replication-inhibiting activity of macrophages in heterokaryons. Our observation represents a kind of "intracellular" cytotoxic activity of macrophages directed against transformed cells.
authors.affiliation: Engelhardt Institute of Molecular Biology, USSR Academy of Sciences, Moscow.
authors.forename: I A
authors.initials: IA
authors.lastname: Prudovsky
authors.forename: R R
authors.initials: RR
authors.lastname: Gumeniuk
authors.forename: Y E
authors.initials: YE
authors.lastname: Yegorov
authors.forename: A V
authors.initials: AV
authors.lastname: Zelenin
mesh_terms: D000818:Animals; D002455:Cell Division; D002459:Cell Fusion; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D004247:DNA / Q000096:biosynthesis; D006367:HeLa Cells; D066298:In Vitro Techniques; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D008808:Mice, Inbred CBA; D051381:Rats
chemical_list: D004247:DNA