A simplified purification method of aminoacylase I.
Taking advantage that the stability of aminoacylase I increases in the presence of Co2+, a simplified method of its purification, which includes a heating step, is described. This method is easy, fast, and gives a good yield of the enzyme. The properties of aminoacylase I thus prepared are similar to those described in the literature.
Hernández
A
A
Department of Biochemistry, School of Medicine, National University of Mexico, D.F., Mexico City.
Piña
E
E
D000581:Amidohydrolases / Q000302:isolation & purification*; D000818:Animals; D003035:Cobalt; D004795:Enzyme Stability; D006358:Hot Temperature; D007668:Kidney / Q000201:enzymology*; D008722:Methods; D013552:Swine
D003035:Cobalt; D000581:Amidohydrolases; C019672:aminoacylase I