Serological and pathogenic characterization of Erysipelothrix rhusiopathiae isolates from tonsils of slaughter pigs in Indonesia.
Erysipelothrix rhusiopathiae was isolated from tonsils of 245 (35.7%) of 687 apparently healthy slaughter pigs in Indonesia during the period of June 1987 to February 1988. A total of 150 of the 245 E. rhusiopathiae isolated could be serotyped within the 22 recognized serotypes. Serotype 2 was most prevalent with 23.7%, followed by Serotypes 11, 12, 1a, 5 and 6 representing 7.3, 5.3, 4.9, 4.9 and 4.1% of the isolates, respectively. The nine other serotypes (Serotypes 1b, 3, 4, 8, 9, 10, 13, 19 and 22) combined to make up 11.0% of the isolates. Antibiotic-resistant strains were not found. Of 86 selected isolates belonging to various serotypes, 76 (88.4%) were highly virulent for mice (LD50 less than 10(3.0) colony-forming units). In swine, 40 (51.2%) of 78 isolates induced local or generalized urticarial lesions after intradermal inoculation, and the remaining 38 isolates induced no clinical signs. Of 76 isolates used for challenge in the cross-protection study, 29 (38.2%) killed greater than 40% of mice immunized with an erysipelas bacterin marketed in Indonesia. A tendency to be refractory to the bacterin-induced immunity was observed in some isolates of various serotypes, but this characteristic was not consistent.
Takahashi
T
T
National Veterinary Assay Laboratory, Tokyo, Japan.
Zarkasie
K
K
Mariana
S
S
Sumadi
Ogata
M
M
D000818:Animals; D003429:Cross Reactions; D004888:Erysipelothrix / Q000145:classification* / Q000276:immunology / Q000472:pathogenicity; D004889:Erysipelothrix Infections / Q000382:microbiology*; D005260:Female; D007214:Indonesia; D051379:Mice; D014066:Palatine Tonsil / Q000382:microbiology*; D012703:Serotyping; D013552:Swine; D013554:Swine Erysipelas / Q000382:microbiology* / Q000517:prevention & control; D014611:Vaccination