Glutamate-like immunoreactivity in calyces of Held.
The pattern of glutamate-like immunoreactivity was investigated in the medial nucleus of the trapezoid body of adult rats. A monoclonal 'anti-glutamate' antibody was combined with postembedding immunohistochemistry involving a silver-intensified peroxidase-antiperoxidase procedure for light microscopy or an immunogold method for electron microscopy. The most conspicuous glutamate-immunoreactive elements were labelled calyces of Held. Because of the outstanding size of these terminals they may provide an attractive model for studying the details of glutamatergic neurotransmission at a synapse of the mammalian CNS.
Grandes
P
P
Brain Research Institute, University of Zurich, Switzerland.
Streit
P
P
D000818:Animals; D000911:Antibodies, Monoclonal; D001306:Auditory Pathways / Q000032:analysis* / Q000648:ultrastructure; D005971:Glutamates / Q000032:analysis*; D007150:Immunohistochemistry; D011149:Pons / Q000032:analysis* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D013569:Synapses / Q000032:analysis* / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D005971:Glutamates