Quantitative autoradiography of dot blots using a microwell densitometer.
We have established conditions for the quantitation of DNA hybridization by "reading" dot blot autoradiographs with a microwell plate densitometer. This method is more convenient, as accurate, and more sensitive than counting the spots in a liquid scintillation counter.
Ross
P M
PM
Rockefeller University, New York, NY 10021.
Woodley
K
K
Baird
M
M
D000818:Animals; D001345:Autoradiography / Q000379:methods*; D001709:Biotechnology; D004247:DNA / Q000032:analysis; D003720:Densitometry / Q000379:methods*; D009693:Nucleic Acid Hybridization; D009710:Nucleotide Mapping / Q000379:methods*; D012588:Scintillation Counting
D004247:DNA