Visualization of predentine matrix components and endocytic structures in rat incisor odontoblasts with tannic acid.
Rat incisor odontoblasts and predentine fixed with tannic acid-glutaraldehyde-osmium tetroxide (Tago) were compared with those obtained by prior incubation in tannic acid-Ringer before conventional fixation with glutaraldehyde-osmium-tetroxide (Tari) The Tago method allowed visualization of complex glycoconjugates along the plasma membrane, in the pericellular spaces and in the intercellular predentine matrix. The non-collagenous proteins, proteoglycans and lipids were seen as granules and thin filaments located between the collagen fibers and at their surface. The collagen fibers themselves were also stained. The Tari method which was used to visualize exocytosis, mainly revealed endocytosis in the form of large intracellular vacuoles containing tannic acid and stained proteoglycans. It is suggested that tannic acid-Ringer incubation prior to fixation increases the endocytosis of the matrix components, which acculumates in these large vesicles.
Goldberg
M
M
Biologie et Biominéralisation des Matrices Extracellulaires, Faculté de Chirurgie Dentaire, Université Paris V, Montrouge.
Septier
D
D
D000818:Animals; D003094:Collagen / Q000032:analysis; D004705:Endocytosis; D006651:Histocytochemistry / Q000379:methods; D047348:Hydrolyzable Tannins / Q000494:pharmacology*; D007180:Incisor / Q000378:metabolism / Q000648:ultrastructure*; D009804:Odontoblasts / Q000378:metabolism / Q000648:ultrastructure*; D011509:Proteoglycans / Q000032:analysis; D051381:Rats; D013634:Tannins / Q000494:pharmacology*
D047348:Hydrolyzable Tannins; D011509:Proteoglycans; D013634:Tannins; D003094:Collagen