A quantitative C-reactive protein assay using latex agglutination in microtiter plates.
In this quantitative assay, latex particles coated with anti-C-reactive protein (CRP) are agglutinated by CRP following vortex agitation in microtiter plates. A decrease in absorbance at 405 nm is directly proportional to CRP concentration. This 30 min assay is simple and necessitates only two pipetting steps after serum dilution. The linear part of the standard curve ranges from 5 to 150 mg/l and CRP concentrations up to 300 mg/l can be determined without additional dilution of sera. Within-assay reproducibility varies from 4.4% to 7.5% while between-assay reproducibility ranges from 9.3% to 12.2%. Correlation studies performed with 104 sera assayed by automated nephelometer and turbidimeter gave correlation coefficients of 0.96 and 0.97 respectively.
Collet-Cassart
D
D
Unit of Experimental Medicine, Faculty of Medicine, Catholic University of Louvain, Brussels, Belgium.
Van den Abbeele
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E
Poncelet
S
S
D002097:C-Reactive Protein / Q000032:analysis*; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D007841:Latex Fixation Tests; D008832:Microchemistry; D009391:Nephelometry and Turbidimetry
D002097:C-Reactive Protein