A kinetic investigation of the acyl-CoA oxidase reaction with the use of a novel spectrophotometric assay. Inhibition by acetyl-CoA, CoA and FMN.
A direct-reading spectrophotometric assay for acyl-CoA oxidase activity is described. The assay is based on the strong absorption at 300 nm of deca-2-trans,4-cis-dienoyl-CoA, the product of oxidation of dec-4-cis-enoyl-CoA. By use of this assay, acetyl-CoA, CoA and FMN were found to be inhibitors of acyl-CoA oxidase, but with distinctly different kinetic characteristics.
Hovik
R
R
Department of Physiology and Biochemistry, Dental School, University of Oslo, Norway.
Osmundsen
H
H
D000105:Acetyl Coenzyme A / Q000494:pharmacology; D042962:Acyl-CoA Oxidase; D000818:Animals; D005486:Flavin Mononucleotide / Q000494:pharmacology; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008297:Male; D008830:Microbodies / Q000201:enzymology; D010088:Oxidoreductases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013056:Spectrophotometry, Ultraviolet
D000105:Acetyl Coenzyme A; D005486:Flavin Mononucleotide; D010088:Oxidoreductases; D042962:Acyl-CoA Oxidase