Inhibition of palatal fusion in vitro by indomethacin in two strains of mice with different H-2 backgrounds.
Susceptibility to glucocorticoid-induced cleft palate in mice has been related to the H-2 histocompatibility complex on chromosome 17. Indomethacin administered in vitro to palatal processes from 13.5-day-old mouse embryos inhibited palatal fusion. Strains with the A background and the H-2a haplotype had significantly higher rates of inhibition than their partners with the H-2b haplotype. The inhibition was prevented in both strains by the addition to the media of prostaglandin E2, but this corrective effect was greater in the A strain with the H-2a haplotype. Thus, blockade of palatal fusion involves prostaglandins, suggesting a similar genetic and biochemical pathway for the different susceptibilities to cleft palate induced by both indomethacin and glucocorticoids.
Montenegro
M A
MA
Department of Experimental Morphology, Faculty of Medicine, University of Chile, Santiago.
Palomino
H
H
D000818:Animals; D002972:Cleft Palate / Q000139:chemically induced* / Q000235:genetics; D015232:Dinoprostone / Q000494:pharmacology*; D004198:Disease Susceptibility; D006239:Haplotypes; D007213:Indomethacin / Q000009:adverse effects*; D008285:Major Histocompatibility Complex / Q000235:genetics*; D051379:Mice; D008805:Mice, Inbred A; D008815:Mice, Inbred Strains; D009924:Organ Culture Techniques; D010159:Palate / Q000187:drug effects*
D015232:Dinoprostone; D007213:Indomethacin