[Evaluation of antigenic fractions of Cysticercus cellulosae for the immunodiagnosis of neurocysticercosis using antibody-lectin conjugates].
In an attempt to find out some fraction with high antigenic activity for the immunodiagnosis of neurocysticercosis a crude extract from Cysticercus cellulosae was fractioned by Sephadex G-200 column chromatography. The protein elution profile revealed two distinct peaks (fractions I and III) and a heterogeneous fraction containing several peaks (fraction II). The crude extract and the fractions were tested by Erythro-Lectin Immuno Test (Erythro-LIT) using cerebrospinal fluid samples from patients with neurocysticercosis. The results of Erythro-LIT antibody titers showed that most of the anticysticercus antibodies recognized antigenic components contained in the fraction II.
Rossi
C L
CL
Departamento de Patologia Clínica, Faculdade de Ciências Médicas, Universidade, Estadual de Campinas, Brasil.
D000909:Antibodies, Helminth / Q000134:cerebrospinal fluid*; D000947:Antigens, Helminth / Q000276:immunology*; D002493:Central Nervous System Diseases / Q000134:cerebrospinal fluid*; D002850:Chromatography, Gel; D003551:Cysticercosis / Q000134:cerebrospinal fluid*; D006801:Humans; D007159:Immunologic Tests; D037102:Lectins
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D037102:Lectins
Avaliação de frações antigênicas de cysticercus cellulosae para o imunodiagnóstico da neurocisticercose utilizando conjugados anticorpo-lectina.