title: Formation of hydroxynorspermidine from exogenously added 1,3-diaminopropan-2-ol in Vibrio species.
abstract: When Vibrio parahaemolyticus AQ 3627 was grown in the presence of 1,3-diaminopropan-2-ol (OH-Dap), a new compound accumulated in the cells. This was identified as hydroxynorspermidine (OH-Nspd), N-(3-aminopropyl)-1,3-diaminopropan-2-ol, by gas chromatography-mass spectrometry and thin-layer chromatography. It was also synthesized enzymatically from OH-Dap by a cell-free preparation from this strain. All other Vibrio strains examined also showed the ability to synthesize this compound, but none of the non-vibrio organisms did, indicating that OH-Dap is an in vivo substrate for the enzyme responsible for biosynthesis of norspermidine characteristically present in vibrios. These results suggest that the ability to synthesize OH-Nspd from OH-Dap present in the growth medium may be useful as an additional identifying factor for the genus Vibrio.
authors.forename: S
authors.initials: S
authors.lastname: Yamamoto
authors.forename: H
authors.initials: H
authors.lastname: Nakao
authors.forename: Y
authors.initials: Y
authors.lastname: Koumoto
authors.forename: K
authors.initials: K
authors.lastname: Takashina
authors.forename: M
authors.initials: M
authors.lastname: Kuroda
authors.forename: S
authors.initials: S
authors.lastname: Shinoda
mesh_terms: D001711:Biotransformation; D011412:Propanolamines / Q000378:metabolism* / Q000493:pharmacokinetics; D013095:Spermidine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D014733:Vibrio / Q000378:metabolism*
chemical_list: D011412:Propanolamines; C063354:hydroxynorspermidine; C017203:1,3-diamino-2-propanol; D013095:Spermidine