Evaluation of type D retroviruses as diagnostic tools in HIV infections.
A type D retrovirus isolated from a permanent human cell line (PMFV) was employed as diagnostic reagent both in Southern transfer hybridization experiments using the cloned genome as a probe and in immunoblot analysis using SDS disrupted virus particles. Hybridization experiments performed under conditions of different stringencies revealed a close homology of PMFV to SAIDS type D retroviruses of serotype 1 (SRV-1, SAIDS retrovirus D/NE), a related homology to the prototype type D virus (MPMV) and to viruses of serotype 2 (SRV-2), but no homology to the endogenous type D retrovirus of squirrel monkeys (SMRV) and the human AIDS virus (HIV-1). Antigens of PMFV showed cross-reactivity only to antibodies of a SAIDS infected macaque, but no reaction to anti HIV-antibodies of seropositive patients. Thus, the type D virus isolated from a human cell line and closely related to SAIDS type D viruses of macaques is not related to the AIDS virus in humans.
Krause
H
H
Department of Virology, Academy of Sciences of the GDR, Berlin.
Kiessig
S
S
Uckert
W
W
D000163:Acquired Immunodeficiency Syndrome / Q000175:diagnosis* / Q000382:microbiology; D015139:Blotting, Southern; D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis*; D015488:HIV Antigens / Q000032:analysis*; D015497:HIV-1 / Q000235:genetics / Q000276:immunology; D006801:Humans; D008970:Molecular Weight; D012190:Retroviridae / Q000145:classification / Q000235:genetics / Q000276:immunology; D012703:Serotyping
D004279:DNA, Viral; D015488:HIV Antigens