[Sex determination of human blood micro-stains and single hairs using specific DNA amplification with polymerase chain reaction].
Amplification of Y chromosome specific DNA in vitro enables a rapid and reliable sex determination of human minute traces such as blood stains and hairs. In presence of male DNA a band of 154 bp is visualized by agarose gel electrophoresis after amplification, this band is lacking in case of female DNA alone. Amplification of a sex independent DNA locus (such as a fragment from the alcohol dehydrogenase gene) generates identical reaction products for both sexes. This shows that the absence of a band is not due to the lack of trace DNA. It is possible to perform this technique with as little as 0.5 microliters of blood or with a single hair.
Muellener
D
D
Gerichtlich-medizinischen Institut der Universität Bern.
Rudin
O
O
Pauli
U
U
Zink
P
P
D001801:Blood Stains; D004247:DNA / Q000235:genetics*; D015342:DNA Probes; D005260:Female; D005784:Gene Amplification / Q000235:genetics*; D006197:Hair / Q000032:analysis*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012732:Sex Determination Analysis
D015342:DNA Probes; D004247:DNA
Geschlechtsbestimmung an menschlichen Mikroblutspuren und Einzelhaaren mit Hilfe gezielter DNA Amplifikation durch die Polymerase-Chain-Reaction.