Evaluation of a colorimetric method for vitamin A estimation.
A simple colorimetric procedure for plasma vitamin A is evaluated, which does not require sophisticated or expensive equipment. Vitamin A values obtained with 30 human plasma samples and 11 liver samples obtained from lactating rabbits, using a colorimetric procedure based on Carr-Price reaction with ferric chloride and acetyl chloride were compared with those obtained with spectrophotometric and spectrofluorimetric micro methods. With widely varying plasma samples, the values showed a high degree correlation and good agreement. The intraassay variation was 3% which is in the acceptable range. The plasma samples could be analysed within 4 weeks and the reagents, were found to be stable, unlike some batches of trifluoro acetic acid (TFA).
Panth
M
M
John
A
A
Sivakumar
B
B
D000818:Animals; D002338:Carotenoids / Q000032:analysis; D003124:Colorimetry / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D011247:Pregnancy; D011817:Rabbits; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D014801:Vitamin A / Q000032:analysis*
D014801:Vitamin A; D002338:Carotenoids