[Flow cytometric studies of anti-granulocyte antibodies].
An indirect immunofluorescence test using anti-granulocyte antibodies was developed to examine many sera samples in a one-step procedure by flow cytometry. Granulocytes obtained from several random donors were fixed with 0.5% paraformaldehyde solution on the first day, and the fluorescence intensity of granulocytes that had reacted with sera was measured on the following day. Non-specific reactions with isoantibodies, i.e., anti-A, anti-B, or serum IgG had no influence on the fluorescence intensity. Therefore, anti-granulocyte antibodies from a patient with immune neutropenia and patients with febrile transfusion reactions can be studied using this method. The results suggest that the IgG-antibody from a patient with immune neutropenia is granulocyte-specific and its subclass is IgG2.
Ohnishi
M
M
D000380:Agranulocytosis / Q000276:immunology*; D000918:Antibody Specificity; D001323:Autoantibodies / Q000032:analysis*; D005434:Flow Cytometry; D006098:Granulocytes / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D009503:Neutropenia / Q000175:diagnosis / Q000276:immunology*
D001323:Autoantibodies; D007074:Immunoglobulin G