Fast atom bombardment and tandem mass spectrometry for structure determination of cysteine, N-acetylcysteine, and glutathione adducts of xenobiotics.
Fast atom bombardment mass spectrometry (FAB/MS) and FAB combined with tandem mass spectrometry (MS/MS) were examined for their applicability to the structure determination of xenobiotics conjugated with the members of the glutathione family (glutathione, cysteine, and N-acetylcysteine). Comparisons between FAB/MS and thermospray MS data are made. FAB/MS is generally successful at generating molecular ion species under both positive and negative ion conditions. Upon collisional activation the adducts undergo characteristic cleavages around the sulfur providing structural information about the conjugate. The analysis of the N-acetylcysteine conjugate isolated from rat urine is presented as an example of the application of FAB/MS/MS to biological problems.
Deterding
L J
LJ
Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.
Srinivas
P
P
Mahmood
N A
NA
Burka
L T
LT
Tomer
K B
KB
D000111:Acetylcysteine; D055598:Chemical Phenomena; D002621:Chemistry; D003545:Cysteine; D005978:Glutathione; D013058:Mass Spectrometry / Q000379:methods; D015394:Molecular Structure; D015262:Xenobiotics
D015262:Xenobiotics; D005978:Glutathione; D003545:Cysteine; D000111:Acetylcysteine