Isolation and structure of two gastrin/CCK-like neuropeptides from the American cockroach homologous to the leucosulfakinins.
Perisulfakinin, a peptide with sequence similarity to gastrin and cholecystokinin, was isolated from the corpora cardiaca of the American cockroach. Its sequence was determined to be Glu-Gln-Phe H-Asp-Asp-Tyr(SO3H)-Gly-His-Met-Arg-Phe-amide. The peptide induced hindgut contractions in the same species at concentrations as low as 250 pM. A related non-sulfated peptide was also isolated and sequenced; it was found to be identical with non-sulfated leucosulfakinin II (pGlu-Ser-Asp-Asp-Tyr-Gly-His-Met-Arg-Phe-amide). This peptide did not stimulate hindgut contractions. The structures of the cockroach peptides of the leucosulfakinin family are thus much more conserved than the cockroach hypertrehalosemic hormones.
Veenstra
J A
JA
Department of Biological Organic Chemistry, C.S.I.C, Barcelona, Spain.
D000595:Amino Acid Sequence; D000818:Animals; D002766:Cholecystokinin; D003058:Cockroaches / Q000032:analysis*; D005755:Gastrins; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000302:isolation & purification*; D012689:Sequence Homology, Nucleic Acid
D005755:Gastrins; D009479:Neuropeptides; C050024:leucosulfakinin; C062380:perisulfakinin; D002766:Cholecystokinin