[Studies on the metabolism of conjugated drugs. III. 3-Deoxyestradiol 17-sulfate. (3). The metabolism by rat liver microsomes].
When 17 beta-hydroxyestra-1,3,5(10)-triene 17-sulfate was incubated with rat liver microsomes, the hydroxylations occurred predominantly at the position 2, followed by the 3. The hydroxylation at 4 was negligible. With proceeding the incubation time, two catechol metabolites hydroxylated at the positions 2,3 and 3,4 were produced. These catechols were confirmed as the second products derived from the above phenolic metabolites. Some kinetic parameters obtained by these hydroxylations showed that all the hydroxylases concerned are different enzymes.
Watanabe
K
K
Wada
Y
Y
Takanashi
K
K
Yoshizawa
I
I
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004958:Estradiol / Q000031:analogs & derivatives* / Q000032:analysis / Q000493:pharmacokinetics; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D051381:Rats
C056171:3-deoxyestradiol 17-sulfate; D004958:Estradiol