Immunohistochemical demonstration of a mRNA-transport protein in rat liver putative preneoplastic foci.
A 65K protein, known for promoting nucleocytoplasmic mRNA transport in a cell-free system, was previously found in fetal and tumor cells of the rat. The primary objective of this study was to show specificity of immunohistochemical staining for the 65K protein in the livers of rats subjected to a hepatocarcinogenesis protocol. Altered hepatic foci were induced by feeding male weanling Sprague-Dawley rats 2-acetylaminofluorene (AAF) followed by a phenobarbital (PB) diet. It was shown, using polyclonal antibodies produced in rabbits, that the 65K protein was present in the cells of rat liver putative preneoplastic foci, with little or none being detected in the surrounding cells.
Hanausek-Walaszek
M
M
Department of Carcinogenesis, University of Texas M.D. Anderson Cancer Center, Smithville, 78957.
Del Rio
M
M
Adams
A K
AK
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis*; D002352:Carrier Proteins / Q000032:analysis*; D007150:Immunohistochemistry; D008099:Liver / Q000032:analysis*; D008114:Liver Neoplasms, Experimental / Q000032:analysis*; D011230:Precancerous Conditions / Q000032:analysis*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D000951:Antigens, Neoplasm; D002352:Carrier Proteins; D012333:RNA, Messenger; C031192:oncofetal antigens