In vivo treatment with erythroid differentiation factor (EDF/activin A) increases erythroid precursors (CFU-E and BFU-E) in mice.
The in vivo effect of human EDF on erythroid precursors (CFU-E and BFU-E) was investigated in normal and bled mice. In anemic (bled) mice, EDF treatment led to significant dose-dependent rises in the CFU-E and BFU-E levels of bone marrow. An elevation in the level of CFU-E was also seen in the spleen. In normal mice, a significant elevation in the level of bone marrow BFU-E was observed. Thus, EDF has an effect on erythropoiesis in anemic and normal mice in vivo.
Shiozaki
M
M
Central Research Laboratories, Ajinomoto Co., Inc., Kawasaki, Japan.
Sakai
R
R
Tabuchi
M
M
Eto
Y
Y
Kosaka
M
M
Shibai
H
H
D028341:Activins; D000740:Anemia / Q000188:drug therapy; D000818:Animals; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D003114:Colony-Forming Units Assay; D004305:Dose-Response Relationship, Drug; D015672:Erythroid Precursor Cells / Q000166:cytology*; D004920:Erythropoiesis / Q000187:drug effects; D006801:Humans; D007265:Inhibins / Q000008:administration & dosage / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D011994:Recombinant Proteins / Q000494:pharmacology; D013154:Spleen / Q000166:cytology
D011994:Recombinant Proteins; D028341:Activins; D007265:Inhibins