[Rapid collection of swine lymphocyte subpopulations in large quantity].
Lymphocyte subpopulations derived from different pig organs (blood, spleen, mesenteric lymph nodes) were separated by a simple, rapid and cheap panning technique, using either normal or ozone treated Petri dishes with bovine serum albumin. Slg+ lymphocytes could thus be obtained with a purity of up to 95% by adhesion onto Corning plastic dishes. The purified cells retained their proliferative activity with regard to lectins. The subpopulation including PT4 and PT8 was then separated by another panning on ozone-treated plastic dishes with a purity of 80-90%.
Charreau
B
B
INRA, Centre de Tours, Laboratoire d'immunologie, Nouzilly, France.
Olivier
M
M
Phan Thanh
L
L
Salmon
H
H
Paraf
A
A
Ruet
B
B
Sage
D
D
D000818:Animals; D002469:Cell Separation / Q000379:methods; D008214:Lymphocytes / Q000166:cytology*; D013552:Swine / Q000097:blood*
Obtention rapide de sous-populations lymphocytaires de porc en grande quantité.