[Effects of opioids on the development of preimplantation mouse embryos].
Opioid peptide DAGO, agonist of opiate mu-receptors and naloxone antagonist of mu-, delta- and kappa-receptors in concentration 3 x 10 M inhibit embryonic development of CBA mice. Inhibition was stage-specific with maximal effect after addition of opioids to zygotes: in the presence of Naloxone no more than 6.7% of embryos reached morula and blastula stages and in the presence of DAGO--36.8%. The other embryos were arrested at two-, four- or, sometimes even, at eight-cell stages without any signs of fragmentation. Four and eight-cell embryos were less sensitive to drug action. Inhibitory effects of these opioids were reduced when they were added simultaneously to zygotes. Agonist of opiate delta-receptors, opioid peptide DADLe, failed to affect embryonic development.
Kaliuzhnyĭ
A E
AE
Platonov
E S
ES
Mironova
O V
OV
Sukhikh
G T
GT
D000818:Animals; D004338:Drug Combinations; D047108:Embryonic Development; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D020875:Enkephalin, Ala(2)-MePhe(4)-Gly(5)-; D004745:Enkephalins / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D051379:Mice; D008808:Mice, Inbred CBA; D009270:Naloxone / Q000008:administration & dosage / Q000494:pharmacology*; D011247:Pregnancy; D015053:Zygote / Q000187:drug effects
D004338:Drug Combinations; D004745:Enkephalins; D020875:Enkephalin, Ala(2)-MePhe(4)-Gly(5)-; D009270:Naloxone
Vliianie opioidov na razvitie predymplantatsionnykh myshinykh zarodysheĭ.