Genetic mapping in Streptomyces clavuligerus by protoplast fusion.
The development of a protoplast manipulation protocol for the industrially important bacterium Streptomyces clavuligerus, which produces the beta-lactamase inhibitor clavulanic acid, made possible a preliminary genetic mapping study based on protoplast fusion crosses. A preliminary position for 11 markers on the S. clavuligerus genetic map is proposed. Fusion progeny were characterized by random spore analysis because the markers present in the strains were not amenable to the conventional four-on-four selection procedure. Whilst the resulting map is similar to that derived by conjugation for S. clavuligerus and S. coelicolor, further analysis of the markers is required to confirm these observations.
Illing
G T
GT
Department of Botany, University of Nottingham, UK.
Normansell
I D
ID
Peberdy
J F
JF
D000483:Alleles; D002874:Chromosome Mapping; D005798:Genes, Bacterial; D011523:Protoplasts / Q000502:physiology; D011995:Recombination, Genetic; D013302:Streptomyces / Q000235:genetics* / Q000502:physiology