A method for horizontal polyacrylamide slab gel electrophoresis.
We present a simplified method of preparation of polyacrylamide gels which is totally analogous to the procedure now widely used to pour and run horizontal agarose gels. The acrylamide is poured into an open air gel mold consisting of a glass plate with a masking tape border and a comb. It is subsequently run in a submarine horizontal electrophoresis apparatus. The electrophoretic mobility and resolution of DNA fragments obtained in such gels are identical to results obtained with gels poured and run in the vertical configuration. Numerous advantages of horizontal polyacrylamide gel electrophoresis are discussed.
Bellomy
G R
GR
Dept. of Biochem., Univ. of Wisconsin-Madison, Madison 53706.
Record
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MT
D001709:Biotechnology; D004247:DNA / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005782:Gels
D005782:Gels; D004247:DNA