Hypelcin A, an alpha-aminoisobutyric acid containing antibiotic peptide, induced permeability change of phosphatidylcholine bilayers.
Interactions of hypelcin A, an alpha-aminoisobutyric acid containing antibiotic peptide, with phosphatidylcholine vesicles were investigated to obtain information on its bioactive mechanism. The peptide induced the leakage of a fluorescent dye, calcein, entrapped in sonicated vesicles. The leakage rate depended on both the peptide and the lipid concentrations. Analysis of this dependency indicated that the leakage was due to the monomeric peptide and that the membrane-perturbing activity of the monomer was higher for solid distearoylphosphatidylcholine vesicles than for fluid egg yolk phosphatidylcholine vesicles. Hypelcin A also affected the gel to liquid-crystalline phase transition of dipalmitoylphosphatidylcholine multilamellar vesicles. The transition was broadened with a reduced transition enthalpy, suggesting the peptide strongly binds the surrounding lipids to perturb the bilayer lipid packing. A circular dichroism study revealed that the helical content of hypelcin A increases upon membrane binding. We concluded that the monomeric peptide with an increased helical content, complexed with the lipids, perturbs the lipid organization and induces the increased permeability.
Matsuzaki
K
K
Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
Nakai
S
S
Handa
T
T
Takaishi
Y
Y
Fujita
T
T
Miyajima
K
K
D000408:Alamethicin / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology*; D000595:Amino Acid Sequence; D000621:Aminoisobutyric Acids / Q000032:analysis*; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology*; D002942:Circular Dichroism; D005452:Fluoresceins / Q000378:metabolism; D007700:Kinetics; D008051:Lipid Bilayers / Q000378:metabolism*; D008969:Molecular Sequence Data; D010539:Permeability; D010713:Phosphatidylcholines / Q000378:metabolism*; D011487:Protein Conformation; D013696:Temperature
D000621:Aminoisobutyric Acids; D000900:Anti-Bacterial Agents; D005452:Fluoresceins; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D000408:Alamethicin; C025478:hypelcin A; C007740:fluorexon