Isolation and characterization of cytoplasmic and nuclear particles of hepatitis B virus.
The properties of hepatitis B virus (HBV) core particles from liver tissue of two patients with acute HBV infection were investigated. Cores were isolated from cytoplasm and nuclear fractions by centrifugation and two thirds of the cores were located in the cytoplasm. In all properties examined cores isolated from the cytoplasm or nucleus were the same. The cores had a density of 1.38 g/ml and had the same DNA and protein content when analyzed by Southern blotting and by Western blotting with C-specific antisera. Cores from both subcellular fractions had endogenous polymerase activity. We conclude that core particles with identical properties are found in both the cytoplasm and nucleus of cells during acute infection.
Theilmann
L
L
Department of Medicine, University of Heidelberg, F.R.G.
Hofmann
W J
WJ
Pfaff
E
E
D015139:Blotting, Southern; D015153:Blotting, Western; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000382:microbiology; D003593:Cytoplasm / Q000382:microbiology; D004279:DNA, Viral / Q000302:isolation & purification; D006509:Hepatitis B / Q000382:microbiology; D006515:Hepatitis B virus / Q000302:isolation & purification*; D006801:Humans; D008099:Liver / Q000382:microbiology; D014764:Viral Proteins / Q000302:isolation & purification
D004279:DNA, Viral; D014764:Viral Proteins