Influence of high and low wall shear rates on the inhibition of factor Xa and thrombin at surfaces coated with immobilized heparin.
The thromboresistant function of a surface with end-point attached heparin is based upon interaction among the immobilized heparin, antithrombin, and at least factor Xa or thrombin. Heparinized arteriovenous shunts were implanted in dogs. By compressing a segment of the shunt, high and low wall shear rate regions were obtained in each shunt. After removal, the tubings were tested for their factor Xa and thrombin inhibitory capacity. It was found that on a molar basis, the factor Xa and thrombin inhibitory capacity were similar in low wall shear rate segments. In high wall shear rate segments, the thrombin inhibitory capacity was decreased, thus indicating that the AT-mediated inhibition of the serine protease is dependent on the wall shear rate.
Arnander
C
C
Department of Experimental Surgery, Karolinska Institute, Stockholm, Sweden.
Pasche
B
B
Kodama
K
K
Rasmuson
A
A
Olsson
P
P
D000818:Animals; D001166:Arteriovenous Shunt, Surgical; D001672:Biocompatible Materials; D001807:Blood Vessel Prosthesis; D004285:Dogs; D015951:Factor Xa; D006493:Heparin; D006801:Humans; D013314:Stress, Mechanical; D013499:Surface Properties; D013917:Thrombin
D001672:Biocompatible Materials; D006493:Heparin; D013917:Thrombin; D015951:Factor Xa