Effect of Zanthoxylum xanthoxyloides and some substituted benzoic acids on glucose-6-phosphate and 6-phosphogluconate dehydrogenases in Hbss red blood cells.
The ether fraction of the aqueous extract of the roots of Zanthoxylum xanthoxyloides (antisickling fraction), vanillic acid, parahydroxybenzoic acid and paraflurobenzoic acid possess antisickling inhibitory activity at low concentrations. Paraflurobenzoic acid was the most active. When the activities of NADP+-linked glucose-6-phosphate and 6-phosphogluconate dehydrogenases in washed Hbss blood specimens treated with these agents (6 mg/ml for antisickling fraction and 6 micrograms/ml for the acids) were compared with controls in vitro, there were no significant differences in either normal or sickled states. The media were devoid of enzyme activity. These agents neither affect the activities of these enzymes while exhibiting antisickling activity nor disrupt the cell membrane to the extent of causing leakage to the media.
Osoba
O A
OA
Department of Pharmacognosy, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, Nigeria.
Adesanya
S A
SA
Durosimi
M A
MA
D000755:Anemia, Sickle Cell / Q000097:blood* / Q000201:enzymology; D000986:Antisickling Agents; D001565:Benzoates / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology*
D000986:Antisickling Agents; D001565:Benzoates; D010936:Plant Extracts; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase