title: Comparative binding of lactate dehydrogenase to mitochondrial fractions.
abstract: Beef liver mitochondrial fraction showed LDH activity (1.76 +/- 0.25 U/g pellet). Sixty seven% of the initial mitochondrial pellet LDH activity (almost M4 isoenzyme) was released when suspended in NaCl 0.15 M. When the washed particles were sonicated in a 0.15 M NaCl medium, the solubilized LDH activity (all five isoenzymes as cytosoluble fraction) was 5-fold higher than the initial pellet activity. The different isoenzymatic composition of intramitochondrial and externally bound forms of the enzyme should be taken into account when investigating the physiological role of intramitochondrial LDH. Beef liver cytosoluble LDH (very little content of M4 isoenzyme) showed no affinity for the beef liver mitochondrial fraction but purified M4-LDH isoenzyme was able to bind to the particulate fraction from the same source. This suggests an isoenzyme specificity for the interaction. The maximum amount of cytosoluble LDH bound to the mitochondrial fraction depends on the enzyme and the particulate fraction source. Therefore, binding capacity to the mitochondrial fraction depends not only on the net charge of LDH isoenzymes, which play a predominant role in the binding, but also on individual characteristics of the LDH isoenzymes and mitochondrial fractions from different sources. This suggests that electrostatic forces are not the only ones involved in the binding process.
authors.affiliation: Department of Biochemistry and Physiology, Faculty of Chemistry, University of Barcelona.
authors.forename: J
authors.initials: J
authors.lastname: Pruñonosa
authors.forename: M L
authors.initials: ML
authors.lastname: Sagristá
authors.forename: J
authors.initials: J
authors.lastname: Bozal
mesh_terms: D000818:Animals; D002417:Cattle; D002645:Chickens; D007526:Isoelectric Point; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000302:isolation & purification / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008930:Mitochondria, Liver / Q000378:metabolism*; D008931:Mitochondria, Muscle / Q000378:metabolism*; D009132:Muscles / Q000201:enzymology*; D011817:Rabbits
chemical_list: D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase