title: Potentiation by the lithium ion of morphogenetic responses to a Xenopus inducing factor.
abstract: We have cultured explants of Xenopus blastular animal cap tissue from embryos that had received an earlier treatment with LiCl and from their untreated siblings, in various concentrations of XTC-cell-derived mesoderm-inducing factor (XTC-MIF, Smith, 1987; Smith et al. 1988). The pretreatment with lithium that we used transforms later morphogenesis in the whole embryo to give radialized body forms with anterior/dorsal levels of structure grossly over-represented. In addition, animal caps from 'Li+' embryos were allowed to develop without exposure to in vitro MIF (Li+ controls) and compared with normal uninduced control explants, and explants were made from normal early blastulae but given various initial treatments with LiCl in culture. The results confirm that the lithium ion itself will not induce mesoderm in competent, animal cap tissue of Xenopus. It does, however, enhance the responsiveness of this tissue to XTC-MIF, in a way that parallels its recently reported effect in the case of another mesoderm inducer of different character, bFGF (Slack et al. 1988). The effects observed are sufficient to imply that the altered body pattern that follows lithium treatment, in whole embryos, could be caused by modulation of the responses to an unaltered pattern of in situ inductive stimuli. We also observe evidence that appreciable inductive signals reach animal pole tissue beyond the limits of mesoderm formation in normal development. Relatively low concentrations of MIF prevent the development of an epidermis-specific marker in dissociated blastular animal cap cells (Symes et al. 1988). When such experiments are repeated in relation to the lithium pretreatment of embryos, such treatment is seen to have sensitized the cell population, so that the MIF concentration range that assures complete suppression of the marker is reduced. The results are discussed in relation to induction considered as pattern formation.
authors.affiliation: Laboratory of Embryogenesis, National Institute for Medical Research, Mill Hill, London, UK.
authors.forename: J
authors.initials: J
authors.lastname: Cooke
authors.forename: K
authors.initials: K
authors.lastname: Symes
authors.forename: E J
authors.initials: EJ
authors.lastname: Smith
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D004625:Embryo, Nonmammalian / Q000648:ultrastructure*; D006133:Growth Substances / Q000494:pharmacology*; D008094:Lithium / Q000494:pharmacology*; D008856:Microscopy, Fluorescence; D009024:Morphogenesis; D014982:Xenopus laevis / Q000196:embryology
chemical_list: D006133:Growth Substances; D008094:Lithium