title: Radioimmunoassay to determine postprandial changes in plasma neuropeptide Y levels in awake dogs.
abstract: A specific, precise and sensitive double-antibody radioimmunoassay for Neuropeptide Y (NPY) has been developed. There was no appreciable cross-reactivity with the structurally related peptides, peptide YY (PYY) and pancreatic polypeptide (PP). The minimum detectable plasma NPY level was 3 nM. Application of radioimmunoassay to canine models revealed that portal and systemic NPY levels increased significantly following a standard meal.
authors.affiliation: Department of Surgery, University of Cincinnati Medical Center, Ohio 45267.
authors.forename: A
authors.initials: A
authors.lastname: Balasubramaniam
authors.forename: D W
authors.initials: DW
authors.lastname: McFadden
authors.forename: M
authors.initials: M
authors.lastname: Rudnicki
authors.forename: M S
authors.initials: MS
authors.lastname: Nussbaum
authors.forename: R
authors.initials: R
authors.lastname: Dayal
authors.forename: L S
authors.initials: LS
authors.lastname: Srivastava
authors.forename: J E
authors.initials: JE
authors.lastname: Fischer
mesh_terms: D000818:Animals; D003429:Cross Reactions; D004285:Dogs; D004435:Eating; D009478:Neuropeptide Y / Q000097:blood* / Q000378:metabolism / Q000592:standards; D011863:Radioimmunoassay / Q000379:methods*
chemical_list: D009478:Neuropeptide Y