6-Thioguanine resistant T-lymphocyte determination as a possible indicator of human ionizing radiation exposure.
We used the autoradiographic assay to assess human in vivo somatic cell gene mutation at the hypoxanthine guanine phosphoribosyl transferase (hgprt) locus in T-lymphocytes. Cells able to incorporate tritiated thymidine in vitro in 6-thioguanine containing short-term cultures were enumerated in order to determine 6-thioguanine resistant (TGr) variant frequencies in cryopreserved lymphocytes from control individuals and 3 persons suspected of 60Co exposure from an accident in Cd. Juárez, México. The data indicate that the lymphocyte TGr variant frequency assay may be potentially usefull for human population monitoring following accidental exposures to ionizing radiation.
Ostrosky-Wegman
P
P
Montero
R
R
Cortinas de Nava
C
C
Hurtado
F
F
D000059:Accidents; D000293:Adolescent; D000328:Adult; D002478:Cells, Cultured; D003037:Cobalt Radioisotopes; D004351:Drug Resistance; D005260:Female; D005796:Genes / Q000528:radiation effects*; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D008297:Male; D008800:Mexico; D008875:Middle Aged; D009154:Mutation; D011834:Radiation Monitoring / Q000379:methods; D013601:T-Lymphocytes / Q000187:drug effects / Q000528:radiation effects*; D013866:Thioguanine / Q000494:pharmacology*
D003037:Cobalt Radioisotopes; D007041:Hypoxanthine Phosphoribosyltransferase; D013866:Thioguanine