Milk protein typing of bovine mammary gland tissue used to generate a complementary deoxyribonucleic acid library.
The milk protein genotype of a mammary gland tissue that was used to generate a cDNA library at the University of California, Davis was typed by PAGE. Casein and whey proteins were extracted from the mammary tissue and typed utilizing fast mini-gel procedures that provide excellent resolution of the milk proteins. The genotype of the mammary tissue was classified as kappa-casein AB, beta-casein A2A1, alpha s1-casein BB, beta-lactoglobulin AA, and alpha-lactalbumin BB. The genes kappa-casein A, beta-casein A2, beta-lactoglobulin A, and alpha-lactalbumin B that have been cloned from this cDNA library coincide with those in the above tissue genotype with the exception of the recently reported cloning of the alpha s1-casein A gene. The gene frequencies for the casein genetic variants for three breeds of dairy cattle is presented and discussed in relation to the low frequency (.3%) of the alpha s1-casein A in the population.
Medrano
J F
JF
Department of Animal Science, University of California, Davis 95616.
Sharrow
L
L
D000483:Alleles; D000818:Animals; D002417:Cattle / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005787:Gene Frequency; D015723:Gene Library; D005838:Genotype; D008321:Mammary Glands, Animal / Q000032:analysis*; D008894:Milk Proteins / Q000235:genetics*
D008894:Milk Proteins; D004247:DNA