title: Occurrence of hybridomas producing multispecific IgM in human x (human-mouse) fusions with lymphocytes from different human immune compartments.
abstract: Human lymphocytes derived from peripheral blood, the spleen and lymph nodes were fused to the HAT-sensitive heteromyeloma cell line CB-F7. The Ig-producing initial cell lines were selected, and the supernatants were further analyzed for specific antigen binding (ELISA). IgM-antibodies were found which reacted with self- and non-self antigens of different molecular origin (nucleotides, proteins, carbohydrate structures). These antibodies were called multireactive (multispecific). The multispecific IgM-producing human hybridomas occurred with higher frequencies in the spleen (6.9% of IgM-producers) cell fusions than in experiments where peripheral blood-derived lymphocytes were fused (2.7%). There were no hybridomas producing multireactive antibodies detected in fusion material from lymph nodes. The greatest number of multireactive IgM was seen when PBL from SLE or anti-HIV-positive patients were hybridized to CB-F7 cells. Representative cell lines were cloned and recloned. The multireactivity of the IgM produced by really monoclonal cells, however, was preserved.
authors.affiliation: Department of Medical Immunology, Medical School, Humboldt University Berlin.
authors.forename: S
authors.initials: S
authors.lastname: Jahn
authors.forename: J
authors.initials: J
authors.lastname: Schwab
authors.forename: S T
authors.initials: ST
authors.lastname: Kiessig
authors.forename: A
authors.initials: A
authors.lastname: Lukowsky
authors.forename: K
authors.initials: K
authors.lastname: Huhnholtz
authors.forename: M
authors.initials: M
authors.lastname: Mehl
authors.forename: U
authors.initials: U
authors.lastname: Specht
authors.forename: R
authors.initials: R
authors.lastname: Grunow
authors.forename: R
authors.initials: R
authors.lastname: von Baehr
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology*; D051379:Mice; D013154:Spleen / Q000166:cytology
chemical_list: D000942:Antigens, Bacterial; D001324:Autoantigens; D007075:Immunoglobulin M