In situ amplification of viral signal in infected samples applied directly to nitrocellulose membranes.
A method for the amplification of viral signal present in infected samples is described. Viral suspensions are spotted on nitrocellulose paper, and immediately afterwards an adequate amount of a permissive cell line is added. The nitrocellulose filter is then incubated overnight, fixed and hybridized to labeled viral probes. The technique is extremely fast, reproducible and inexpensive, and may be readily applicable to the clinical diagnosis of many viral diseases.
De Stasio
P R
PR
Department of Biology, Indiana University, Bloomington 47405.
Etchberger
K J
KJ
Paeratakul
U
U
Taylor
M W
MW
D001709:Biotechnology; D003101:Collodion; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005374:Filtration / Q000295:instrumentation; D015335:Molecular Probes; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D014773:Virology / Q000379:methods*; D014780:Viruses / Q000235:genetics / Q000302:isolation & purification*
D004279:DNA, Viral; D015335:Molecular Probes; D012367:RNA, Viral; D003101:Collodion