[Evaluation of the anti-ulcer drugs using image analysis technology: effect of aldioxa containing preparation on the experimental gastric ulcer in rats].
Direct measurements under a microscope have been employed to evaluate experimental gastric ulcers, but the following problems have been left unsettled: it takes too much time, and much experience is required to perform accurate, objective measurements. The present study demonstrates the usefulness of image analysis technology for evaluating anti-ulcer drugs. With this newly developed method, the region of gastric mucosal injury can be automatically and/or mechanically chosen on the basis of three different factors: intensity, hue and purity. Consequently, this method can be used to rapidly obtain analytical data such as the number, area and length of gastric ulcers. Effect of NNP, a preparation containing aldioxa, on three different types of experimental ulcer formation (stress-induced, ethanol-induced, and pylorus-ligation) was studied by image analysis technology. In all cases, NNP (2,562 mg/kg, p.o.) almost completely inhibited the formation of rat gastric ulcer. From the finding that image analysis technology is very useful for evaluating anti-ulcer drugs, we conclude that this method will help further the development of anti-ulcer drugs.
Umemoto
M
M
Research Center Rohto Pharmaceutical Co., Ltd., Osaka, Japan.
Nakamura
S
S
Okumura
S
S
Sekiguchi
T
T
Nishihara
T
T
Nishimura
T
T
Hayashi
S
S
D000481:Allantoin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000627:therapeutic use; D000536:Aluminum Hydroxide / Q000008:administration & dosage / Q000627:therapeutic use*; D000818:Animals; D000863:Antacids / Q000008:administration & dosage / Q000627:therapeutic use*; D004341:Drug Evaluation / Q000379:methods; D000431:Ethanol; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008026:Ligation; D008297:Male; D051381:Rats; D013276:Stomach Ulcer / Q000188:drug therapy* / Q000473:pathology; D013312:Stress, Physiological
D000863:Antacids; D000481:Allantoin; D000431:Ethanol; D000536:Aluminum Hydroxide; C017231:aldioxa