Some properties of bovine mitochondrial serine tRNA gene transcript synthesized with T7 RNA polymerase.
Bovine mitochondrial serine tRNA(AGY) gene transcript was synthesized in vitro with T7 RNA polymerase, and it was capable of being aminoacylated with mitochondrial serine tRNA synthetase. The melting profiles of the transcript was similar to those of native serine tRNA(AGY), suggesting that the higher-order structure of the transcript does not much differ from that of native serine tRNA(AGY). Several transcripts with base-substitution were also constructed and their aminoacylation capacity was investigated.
Ueda
T
T
Department of Life Chemistry, Graduate School at Nagatsuta, Tokyo Institute of Technology, Yokohama, Japan.
Watanabe
K
K
D000818:Animals; D002417:Cattle; D012321:DNA-Directed RNA Polymerases; D008928:Mitochondria / Q000502:physiology; D009154:Mutation; D012345:RNA, Transfer, Amino Acid-Specific / Q000235:genetics*; D012362:RNA, Transfer, Ser / Q000096:biosynthesis / Q000235:genetics*; D013604:T-Phages / Q000201:enzymology / Q000235:genetics; D014158:Transcription, Genetic
D012345:RNA, Transfer, Amino Acid-Specific; D012362:RNA, Transfer, Ser; D012321:DNA-Directed RNA Polymerases