title: Quantitative determination of arecoline in plasma by gas chromatography chemical ionization mass spectrometry.
abstract: A capillary gas chromatography/mass spectrometry (GC/MS) method for the quantitative analysis of arecoline in plasma has been developed for concentrations in the range 1-50 ng ml-1. Hexadeuterated arecoline was utilized as the internal standard. The removal of drug from plasma was accomplished by a two-step liquid/liquid extraction procedure involving a wash step followed by extraction with 5% triethyl amine in ethyl acetate. The GC/MS determinations were carried out with temperature-programmed capillary GC and ammonia chemical ionization mass spectrometry. The [M + H]+ ions of both analyte and internal standard were monitored at m/z 156 and 162, respectively. The method is linear and has sufficient sensitivity, precision, accuracy and selectivity for analysis of drug levels in human plasma.
authors.affiliation: Development Department, CIBA-GEIGY Corporation, Ardsley, New York 10502.
authors.forename: M J
authors.initials: MJ
authors.lastname: Hayes
authors.forename: L
authors.initials: L
authors.lastname: Khemani
authors.forename: M
authors.initials: M
authors.lastname: Bax
authors.forename: D
authors.initials: D
authors.lastname: Alkalay
mesh_terms: D001115:Arecoline / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans
chemical_list: D001115:Arecoline