Antiviral activities and the mechanism of 9-beta-D-ribofuranosyl-6-alkylthiopurines on several RNA viruses from animals.
A series of 9-beta-D-ribofuranosyl-6-alkylthiopurines (6-alkyl TI) were found to inhibit in vitro replication of infectious hematopoietic necrosis virus (IHNV), human influenza virus (IFV) and respiratory syncytial virus (RSV) with IC50 values of about 0.06 microgram/ml, 0.7-1.5 micrograms/ml and 1-3 micrograms/ml, respectively. Viral RNA synthesis in infected cells in the presence of actinomycin D was inhibited by treatment with the compounds dose-dependently. It was also found that the decrease of rNTP pool size in infected cells was remarkably dose-dependent. From these findings, the mode of antiviral action of these compounds may be explained by rNTP imbalance in the treated group.
Saneyoshi
M
M
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Hasobe
M
M
Yamamoto
K
K
D000998:Antiviral Agents; D011684:Purine Nucleosides / Q000494:pharmacology*; D012328:RNA Viruses / Q000187:drug effects*; D012263:Ribonucleosides / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D013872:Thionucleosides / Q000494:pharmacology*
D000998:Antiviral Agents; D011684:Purine Nucleosides; D012263:Ribonucleosides; D013872:Thionucleosides; C005248:nebularine