Application of a biotin-labelled DNA probe to detect Campylobacter.
A procedure for direct detection of Campylobacter in feces has been developed using a biotin-labelled DNA probe. A species-specific probe (Bio-pCJ174 probe) for C. jejuni and a multivalent probe (Bio-pooled probe) for the thermotolerant Campylobacter species were developed with Bio-11-dUTP. Both probes were used to detect Campylobacter in 100 specimens of chicken feces. The coincidence rate of the results obtained by the Bio-pooled probe and cultivation was 90% and that of the Bio-pCJ 174 probe and cultivation was 96%. Identification of isolates was also carried out with both probes.
Zhou
Y T
YT
Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing.
Wang
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SQ
D000818:Animals; D001710:Biotin; D002167:Campylobacter / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002168:Campylobacter fetus / Q000235:genetics / Q000302:isolation & purification*; D002645:Chickens; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D005243:Feces / Q000382:microbiology*; D006801:Humans; D009693:Nucleic Acid Hybridization; D011237:Predictive Value of Tests; D013045:Species Specificity
D015342:DNA Probes; D004269:DNA, Bacterial; D001710:Biotin