title: [Oxaloacetate keto-enol tautomerase from bovine heart mitochondrial matrix].
abstract: Bovine heart mitochondrial matrix contains two proteins possessing the oxaloacetate keto-enol tautomerase (EC 5.3.2.2) activity. A procedure for the isolation and purification of the enzymes to an electrophoretically homogeneous state has been developed. The purified proteins have molecular masses of 37 kD and 80 kD and catalyze the keto-enol oxaloacetate tautomerization reaction with the turnover numbers of approximately 3000 and approximately 2000 min-1. The both enzymes were found to differ significantly in all their physicochemical and kinetic properties. Fractionation of rat liver mitochondria revealed that the oxaloacetate keto-enol tautomerase activity is predominantly localized in the mitochondrial matrix. The essential role of oxaloacetate keto-enol tautomerase in the operation of the Krebs cycle is discussed.
authors.forename: V I
authors.initials: VI
authors.lastname: Burov
authors.forename: Iu O
authors.initials: IuO
authors.lastname: Belikova
authors.forename: A D
authors.initials: AD
authors.lastname: Vinogradov
mesh_terms: D000818:Animals; D002384:Catalysis; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D019746:Intramolecular Oxidoreductases; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism; D007535:Isomerases / Q000302:isolation & purification* / Q000378:metabolism; D007700:Kinetics; D008929:Mitochondria, Heart / Q000201:enzymology* / Q000648:ultrastructure; D008970:Molecular Weight; D010071:Oxaloacetates / Q000378:metabolism*; D013385:Succinate Dehydrogenase / Q000378:metabolism
chemical_list: D007527:Isoenzymes; D010071:Oxaloacetates; D013385:Succinate Dehydrogenase; D007535:Isomerases; D019746:Intramolecular Oxidoreductases; C048474:oxaloacetate tautomerase
vernacular_title: Oksaloatsetat keto-enol tautomerazy matriksa mitokhondriĭ serdtsa byka.