Glandular kallikrein-like enzyme in adrenal glands.
A kallikrein-like kininogenase was identified in the rat adrenal gland. Most of the enzyme was present in an inactive form, since pre-incubation with trypsin markedly increased kininogenase activity from 54.8 +/- 11.8 to 230 +/- 23.0 pg bradykinin/mg protein/min. Adrenal kininogenase was inhibited 90% by phenyl methyl sulfonyl fluoride, 92% by D-Phe-Phe-Arg-chloromethylketone, 91% by aprotinin, and only 15% by soybean trypsin inhibitor. Pre-incubation with antibodies against rat urinary kallikrein resulted in 85% inhibition. The apparent molecular weight of adrenal kininogenase on gel filtration chromatography was 33 Kd. The enzyme was strongly adsorbed to immobilized rat urinary kallikrein antibodies and required drastic conditions for elution. In canine adrenal glands, we found that there was no difference in the cortical and medullary distribution of active and inactive SBTI resistant kininogenase activity. We conclude that an enzyme which closely resembles glandular kallikrein is present in adrenal glands.
Scicli
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Hypertension Research Division, Henry Ford Hospital, Detroit, MI.
Nolly
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Carretero
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OA
Scicli
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AG
D000302:Adrenal Cortex / Q000201:enzymology; D000311:Adrenal Glands / Q000201:enzymology*; D000313:Adrenal Medulla / Q000201:enzymology; D000818:Animals; D002846:Chromatography, Affinity; D004789:Enzyme Activation; D007610:Kallikreins / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D008970:Molecular Weight; D011480:Protease Inhibitors / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D014357:Trypsin
D011480:Protease Inhibitors; D007610:Kallikreins; D014357:Trypsin