Molecular diagnosis of chronic myeloid leukemia using a 3'bcr probe.
Chronic myeloid leukemia (CML) is characterized by the Philadelphia chromosome which results from a reciprocal (9; 22) translocation, with the protooncogene c-abl moving from chromosome 9 to 22 and juxtaposed to the proximal bcr. Breakpoints on chromosome 22 are localized within 5.8 kb of the breakpoint cluster region (bcr). We have assessed the feasibility of using a 3'bcr probe for molecular diagnosis of CML. Thirty patients with Ph chromosome negative or positive CML were studied by Southern blot. A bcr rearrangement was seen to be present in all but one patient with Ph+CML. A case of Ph negative CML showed a bcr rearrangement. We conclude that this technique is efficient for molecular diagnosis of CML.
Berthéas
M F
MF
Département d'Hématologie et d'Immunologie, CHU, Saint-Etienne, Hôpital Nord, Saint-Priest-en-Jarez, France.
Vindimian
M
M
Ollagnon
G
G
Pomier
G
G
Jaubert
J
J
Vasselon
C
C
Reynaud
J
J
Heally
J C
JC
Le Petit
J C
JC
Brizard
C P
CP
D015139:Blotting, Southern; D015342:DNA Probes; D015321:Gene Rearrangement / Q000235:genetics; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000175:diagnosis* / Q000235:genetics
D015342:DNA Probes