title: [Use of spin probe in the determination of oxygen consumption in human neutrophils].
abstract: A quantitative method of the oxygen consumption rate measurement by human blood neutrophils upon their activation is described. This method is based on the spin exchange determination between 3-carbamoyl-2,2,5,5-tetramethyl-3-pyrroline-1-yloxyl spin probe and oxygen molecules from the ESR spectra. Method allows the determination of about 1 micromolar concentrations of oxygen.
authors.forename: A N
authors.initials: AN
authors.lastname: Osipov
authors.forename: O A
authors.initials: OA
authors.lastname: Kulikov
authors.forename: O A
authors.initials: OA
authors.lastname: Azizova
mesh_terms: D003497:Cyclic N-Oxides; D006801:Humans; D009504:Neutrophils / Q000032:analysis* / Q000378:metabolism; D010100:Oxygen / Q000097:blood; D010101:Oxygen Consumption; D013113:Spin Labels
chemical_list: D003497:Cyclic N-Oxides; D013113:Spin Labels; C047253:3-carbamoyl-2,2,5,5-tetramethyl-3-pyrroline-1-yloxyl; D010100:Oxygen
vernacular_title: Ispol'zovanie spinovogo zonda dlia izmereniia potrebleniia kisloroda neĭtrofilami cheloveka.