[Composition and immunological activity of membrane-bound surface glycoprotein from Crithidia oncopelti].
A membrane-bound glycoprotein with a molecular weight of 10000-12000 was isolated from Crithidia oncopelti and purified. The glycoprotein contained peptide, carbohydrate and lipid fragments and phosphorus. The peptide fragment was represented by 10 amino acids. The carbohydrate fragment was represented by 7 monosaccharides. The lipid part was mainly represented by stearic acid. The glycoprotein showed immunostimulating properties. It had a comitogenic effect on murine spleen cells in vitro and induced tumoricidal activity in murine peritoneal macrophages in vitro and in vivo.
Sukhareva
N N
NN
Zaretskaia
M Sh
MSh
Khorokhorina
V A
VA
Egorov
N S
NS
Viadro
M M
MM
Titova
T S
TS
Fomina
I P
IP
D000276:Adjuvants, Immunologic; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D003421:Crithidia / Q000378:metabolism*; D066298:In Vitro Techniques; D007941:Leukemia P388 / Q000276:immunology*; D007942:Leukemia, Experimental / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000032:analysis / Q000276:immunology*; D051379:Mice; D009005:Monosaccharides / Q000032:analysis; D013154:Spleen / Q000166:cytology; D013229:Stearic Acids / Q000032:analysis
D000276:Adjuvants, Immunologic; D000596:Amino Acids; D008562:Membrane Glycoproteins; D009005:Monosaccharides; D013229:Stearic Acids
Sostav i immunologicheskaia aktivnos't membranosviazannogo poverkhnostnogo glikoproteina Crithidia oncopelti.