[Proliferative activity of granulocyte progenitors from the bone marrow of patients with chronic myeloid leukemia in 14-day culture in vivo].
The proliferative activity of marrow granulocyte progenitors (CFU-dG) from patients with chronic myeloid leukemia (CML) and healthy subjects was investigated in 14th-day culture using diffusion chamber technique in vivo. On each day the number of clusters (20-50 cells) and colonies (over 50 cells) was estimated. In addition, on the day 4, 7 and 9 the count of CFU-dG in the S-phase of cell cycle was determined. During the first 9 days the number of clusters and colonies successively increased both in CML and control, but this result was more pronounced in experiments with CML-CFU-dG. In the period between days 9 th and 14th a decrease in the proliferative activity of CFU-dG could be observed. During the whole culture-time the number of colonies was markedly higher than that of clusters. The percentage of CML-CFU-dG in S-phase was comparable to that noted in controls.
Hansz
J
J
Kozłowska-Skrzypczak
M
M
D000328:Adult; D001853:Bone Marrow / Q000473:pathology*; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D015194:Diffusion Chambers, Culture; D005260:Female; D006098:Granulocytes / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D013997:Time Factors
Aktywność proliferacyjna komórek ukierunkowanych szeregu granulocytarnego szpiku chorych na przewlekła białaczke szpikowa w 14-dniowej hodowli in vivo.