Effect of ethanol on lipofuscin accumulation in cultured rat cardiac myocytes.
The objective of this study was to determine the effect of ethanol on in vitro life span, rate of contraction and lipofuscin content of neonatal rat cardiac myocytes. Lipofuscin was quantified by microspectrofluorometry. The effects of 0, 3.1, 6.5, and 12.5 mM ethanol on myocytes, kept under an ambient oxygen concentration of 20% and 40%, were studied. Exposure to low concentrations of ethanol resulted in a decrease in the amount of lipofuscin whereas exposure to high concentration of ethanol caused an increase in the level of lipofuscin. The length of cell survival in controls and 3.1 mM ethanol exposed myocytes was similar under 20% oxygen, but was longer in the latter group under 40% oxygen, as compared to controls. The total number of contractions in 3.1 mM ethanol-exposed myocytes were, respectively, 4% and 8% higher under 20% and 40% oxygen atmosphere than in control cells.
Sohal
R S
RS
Department of Biological Sciences, Southern Methodist University, Dallas TX 75275.
Marzabadi
M R
MR
Brunk
U T
UT
D000367:Age Factors; D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D000431:Ethanol / Q000494:pharmacology*; D005609:Free Radicals; D006321:Heart / Q000187:drug effects; D008062:Lipofuscin / Q000493:pharmacokinetics*; D009119:Muscle Contraction / Q000187:drug effects; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D010100:Oxygen / Q000494:pharmacology; D010860:Pigments, Biological / Q000493:pharmacokinetics*; D051381:Rats; D011919:Rats, Inbred Strains
D005609:Free Radicals; D008062:Lipofuscin; D010860:Pigments, Biological; D000431:Ethanol; D010100:Oxygen