[Growth and contractility of cells from diabetic pre-retinal membrane in primary tissue culture].
Cells grew out in tissue culture from 7 out of 12 diabetic pre-retinal membrane specimens. Cell types of epithelium, glia, macrophage, blood vessel, and fibroblast-like cells were distinguished by inverted phase microscopy, electron-microscopy and immunostaining. Electron-microscopy demonstrated abundant cytoplasm fibrils in some of the cells, and immunostaining for actin showed great variation in the intensity of staining. An Mg++ ATP contraction assay on 81 detergent-treated cells revealed a 25% average reduction in the cell area. Immunostaining for cytokeratin and GFAP following the Mg++ ATP assay showed no significant difference in cell contraction among epithelial, glial and other cells. The role of fibroblast-like cells and the contraction of pre-retinal membrane were discussed.
Jiang
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DY
D000199:Actins / Q000502:physiology; D046508:Culture Techniques; D003930:Diabetic Retinopathy / Q000473:pathology*; D006651:Histocytochemistry; D006801:Humans; D012160:Retina / Q000473:pathology*
D000199:Actins