title: Morbillivirus infection among seals (Phoca vitulina) during the 1988 epidemic in the Bay of Heligoland. II. Serogological investigations reflecting a previous phocine distemper epidemic in a seal orphanage.
abstract: Of 57 seals hospitalized in the Norddeich Orphanage, 37 (65%) had died until 22nd of August, 1988, when the first collection of blood samples from the survivors commenced. All the sera including those obtained at a later date from the remaining animals had invariably higher neutralizing antibody titres against a phocine distemper virus (PDV) isolate than against canine distemper virus (CDV). The difference of mean titres was calculated to be more than 1.5 x log10 serum dilution. Peak titres demonstrated by a direct neutralization peroxidase-linked antibody (NPLA) assay reached 1/90,000. Attempts to isolate PDV in seal kidney cell cultures from heparinized blood samples collected from convalescent animals were not successful. From the increase in antibody titre following the last fatal case it was concluded that the devastating epidemic sweeping through the Norddeich Orphanage was primarily due to phocine distemper.
authors.forename: B
authors.initials: B
authors.lastname: Liess
authors.forename: H R
authors.initials: HR
authors.lastname: Frey
authors.forename: T
authors.initials: T
authors.lastname: Willhaus
authors.forename: A
authors.initials: A
authors.lastname: Zaghawa
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D010872:Caniformia; D004196:Disease Outbreaks / Q000662:veterinary*; D004217:Distemper Virus, Canine / Q000276:immunology; D009500:Neutralization Tests; D010252:Paramyxoviridae / Q000276:immunology*; D010253:Respirovirus Infections / Q000453:epidemiology / Q000662:veterinary*; D046048:Seals, Earless
chemical_list: D000914:Antibodies, Viral