title: Changes in collagen synthesis by human bone marrow fibroblasts with progressive subcultivation.
abstract: Bone marrow fibroblasts from normal and leukemic patients were used to investigate the relationship between serial subcultivation and changes in collagen synthesis. A regime was established to generate subcultures up to 35 cumulative population doublings (CPDs) in normal cells and to 9 CPDs in leukemic cells. In both types of cells, collagen synthesis decreased as subcultivation progressed. In normal cells, collagen synthesis was reduced to 10% of the original levels at 18 CPDs and in leukemic cells at 8 CPDs. In normal fibroblasts, collagen synthesis was more profoundly affected than overall protein synthesis by subcultivation. In acute lymphoblastic leukemia-derived fibroblasts, the decrease in collagen synthesis paralleled that of total protein.
authors.affiliation: Unidad de Biologia Celular, Universidad de Chile, Santiago.
authors.forename: M
authors.initials: M
authors.lastname: Fernandez
authors.forename: C
authors.initials: C
authors.lastname: Barahona
authors.forename: J
authors.initials: J
authors.lastname: Martinez
authors.forename: J J
authors.initials: JJ
authors.lastname: Minguell
mesh_terms: D001853:Bone Marrow / Q000378:metabolism*; D001854:Bone Marrow Cells; D002455:Cell Division; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D003094:Collagen / Q000378:metabolism*; D005109:Extracellular Matrix / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006410:Hematopoiesis; D006801:Humans
chemical_list: D003094:Collagen