title: Improved long-term storage of hybridomas at -80 degrees C using a bovine milk derivative.
abstract: A medium comprising 40% bovine milk fraction and 10% DMSO (medium A) was used for the long-term storage of hybridomas at -80 degrees C. The viability of the cells, their growth recovery and ability to secrete antibody were studied and the results were compared to those obtained after storage in a medium containing 40% fetal calf serum and 10% of DMSO (medium B). Hybridomas have been kept for 2 years in medium A; the viability of such cells was 75%, the cells were healthy (electron microscopy), they rapidly proliferated when they were cultured in RPMI supplemented with 10% FCS or with 9% milk fraction + 1% FCS and they released measurable levels of antibody. In contrast, hybridomas stored under the same conditions but in medium B died after 6 months.
authors.affiliation: Biochemistry Laboratory School of Medicine, Faculty of Sciences, Nancy University I, Vandoeuvre, France.
authors.forename: F
authors.initials: F
authors.lastname: Derouiche
authors.forename: J M
authors.initials: JM
authors.lastname: Bour
authors.forename: C
authors.initials: C
authors.lastname: Legrand
authors.forename: J
authors.initials: J
authors.lastname: Capiaumont
authors.forename: F
authors.initials: F
authors.lastname: Belleville
authors.forename: G
authors.initials: G
authors.lastname: Linden
authors.forename: P
authors.initials: P
authors.lastname: Nabet
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D002455:Cell Division; D002470:Cell Survival; D003451:Cryoprotective Agents; D005615:Freezing; D006825:Hybridomas; D066298:In Vitro Techniques; D051379:Mice; D008854:Microscopy, Electron; D008892:Milk; D008970:Molecular Weight; D011309:Preservation, Biological / Q000379:methods*; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D003451:Cryoprotective Agents