Transplantation of nuclei from 2- to 16-cell embryos into enucleated blastomeres of 2-cell mouse embryos.
Individual blastomeres of 2-, 4-, 8- and 16-cell embryos were fused with enucleated blastomeres of 2-cell embryos. The percentage of fused nuclear donor/recipient pairs was high (92 to 100%). Most of the reconstituted embryos cleaved twice within 24 h of in vitro culture and 83% to 95% developed to the morula or blastocyst stage. Increasing the number of blastomeres in reconstituted embryos by the construction of aggregates from blastomeres obtained 24 h after nuclear donor/recipient fusion was effective. All constructed aggregates reached the morula or blastocyst stage within 30 h of in vitro culture. The normal day-13 implantations were recorded after the transfer of reconstituted "half" embryos and aggregates when 8-cell blastomeres were used as nuclear donors.
Landa
V
V
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Praha.
D000818:Animals; D001755:Blastocyst / Q000166:cytology; D001757:Blastomeres / Q000166:cytology*; D002459:Cell Fusion; D002467:Cell Nucleus / Q000502:physiology; D002970:Cleavage Stage, Ovum; D046508:Culture Techniques; D004624:Embryo Transfer; D005314:Embryonic and Fetal Development; D005260:Female; D008297:Male; D051379:Mice; D009028:Morula / Q000166:cytology; D053652:Nuclear Transfer Techniques