Synthesis and properties of the box 9R and 9R' sequences of Tetrahymena rRNA.
The chloroethoxyethyl (CEE) group is completely stable under the acidic conditions required to remove the 5'-protecting groups in the oligoribonucleotide synthesis, but can be cleaved under the similar condition to that of the tetrahydropyranyl (THP) group in region of pH 2-3. The oligoribonucleotides were synthesized by the phosphoramidite method on solid supports.
Yamakage
S
S
Laboratory of Bioorganic Chemistry, Department of Industrial Chemistry, Chiba Institute of Technology, Japan.
Sakatsume
O
O
Takaku
H
H
D000818:Animals; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D012335:RNA, Ribosomal / Q000138:chemical synthesis*; D013768:Tetrahymena / Q000235:genetics*; D014529:Uridine / Q000031:analogs & derivatives / Q000138:chemical synthesis
D009843:Oligoribonucleotides; D012335:RNA, Ribosomal; D014529:Uridine