[Imaging and biodistribution of 111In-labeled anti-carcinoembryonic antigen monoclonal antibodies in nude mice bearing human colon cancer xenografts].
Anti-CEA monoclonal antibody was labeled with 111In using DTPA cyclic anhydride. The radiochemical purity of the product was more than 99% and specific binding was 38%. Imaging and biodistribution of this radiopharmaceutical in nude mice bearing human colon cancer xenografts were investigated. The results showed that a clear image of tumor was obtained by gamma camera between 24 to 120 hrs, best in 72 hrs, after injection. A high uptake of 111In-labeled monoclonal antibodies in tumor tissue was also observed, although the radioactivity in liver was considerable.
Wu
H
H
Peking Union Medical College Hospital, Beijing.
D000818:Animals; D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen / Q000032:analysis* / Q000276:immunology; D003110:Colonic Neoplasms / Q000000981:diagnostic imaging* / Q000276:immunology; D015902:Gamma Cameras; D007205:Indium Radioisotopes; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011877:Radionuclide Imaging; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen; D007205:Indium Radioisotopes