An explanation of the noncorrespondence between assessment methods of cyclosporin.
Various methods of determining cyclosporin (CyA) levels in patients after kidney transplantation were compared. These included polyclonal antibody (pcAb-), specific and nonspecific monoclonal antibody (S- and NmcAb-) radioimmunoassays (RIA), and high performance liquid chromatography (HPLC). The results obtained by the various methods when compared showed some correlation but did not correspond. A probable explanation for part of this noncorrespondence is the presence of monoclonally crossreactive metabolites (CyA-M). Another reason was that the concentration of CyA in the standards supplied with the RIA kits was found to be higher than stated.
Randleff-Rasmussen
G M
GM
Department of Surgery, University of the Witwatersrand Medical School, Johannesburg, South Africa.
Oosthuizen
M M
MM
Myburgh
J A
JA
D000911:Antibodies, Monoclonal; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003429:Cross Reactions; D003524:Cyclosporins / Q000097:blood* / Q000276:immunology / Q000592:standards; D006801:Humans; D016030:Kidney Transplantation; D011863:Radioimmunoassay / Q000379:methods
D000911:Antibodies, Monoclonal; D003524:Cyclosporins; C050026:cyclosporin D