title: Repair of Hymenolepis diminuta after complement-mediated damage.
abstract: Seven and 56-day-old Hymenolepis diminuta were exposed to complement by incubation in 50% normal rat serum (NRS) in modified Hanks' saline. Ultrastructural studies revealed that the scolex/neck region remained relatively intact whilst in the strobila region microthrix denudation and loss of distal cytoplasm were observed. When complement-mediated damaged worms were incubated in vitro in 50% heat-inactivated normal rat serum (hiNRS) plus M199 or implanted into the duodenum of NMRI mice repair occurred, although destrobilated parasites were only found in vivo. The regions undergoing repair contained tegumental protrusions, vesicles, large electron-lucent areas and large quantities of lipid. Microtriches were formed parallel to the parasite surface and were raised into a perpendicular position. It is suggested that the regenerative process exhibited after complement-mediated damage does not mimic totally the embryological development of the surface layer.
authors.affiliation: Department of Parasitology, University of Copenhagen, Denmark.
authors.forename: J
authors.initials: J
authors.lastname: Andreassen
authors.forename: D
authors.initials: D
authors.lastname: Hoole
mesh_terms: D000818:Animals; D003165:Complement System Proteins / Q000276:immunology*; D006926:Hymenolepis / Q000502:physiology* / Q000648:ultrastructure; D012038:Regeneration
chemical_list: D003165:Complement System Proteins