[Epidermal growth factor induces the progesterone receptor in fetal uterine cells in culture: antagonistic effect of antiestrogens].
Cells isolated from the uterus of the guinea pig foetus can be maintained in culture even through several sub-cultures. In these cells, estradiol increases progesterone receptor concentrations 2 o 3 times, although estradiol has no effect on cell proliferation. Epidermal growth factor (EGF) stimulates both cell proliferation and the progesterone receptor 4-Hydroxytamoxifen, a tamoxifen metabolite and potent anti-oestrogen, completely inhibits the stimulatory effect of EGF on the progesterone receptor but has no effect on the EGF-induced cell growth. These cells have specific binding sites with high affinity for 125I-EGF. Estradiol increases the number of binding sites but does not affect the affinity for EGF. 4-Hydroxytamoxifen has no significant effect on either the number of binding sites or the binding affinity. In conclusion, EGF could be an autocrine or paracrine factor in estrogen-sensitive cells not only as a potent mitogen but also as a factor capable of increasing an estrogen-induced protein like the progesterone receptor. The observation that an anti-estrogen can also act as an "anti-growth factor" suggests a close relationship between estrogens and growth factors.
Sumida
C
C
Equipe de Recherche du CNRS, n. UA-548, Fondation de Recherche en Hormonologie, Paris, France.
Pasqualini
J R
JR
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000378:metabolism / Q000494:pharmacology*; D066246:ErbB Receptors / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology; D004965:Estrogen Antagonists / Q000494:pharmacology*; D005260:Female; D006168:Guinea Pigs; D011980:Receptors, Progesterone / Q000096:biosynthesis*; D013629:Tamoxifen / Q000031:analogs & derivatives / Q000494:pharmacology; D014599:Uterus / Q000187:drug effects / Q000196:embryology* / Q000378:metabolism
D004965:Estrogen Antagonists; D011980:Receptors, Progesterone; D013629:Tamoxifen; C016601:afimoxifene; D004958:Estradiol; D004815:Epidermal Growth Factor; D066246:ErbB Receptors
Le facteur de croissance épidermique induit le récepteur de la progestérone dans les cellules de l'utérus foetal en culture: effet antagoniste des anti-oestrogènes.