Variation in the effects of mononuclear cell products from different individuals on metalloproteinase secretion from human articular cartilage.
Human articular cartilage in organ culture shows a variable degree of endogenous metalloproteinase secretion depending on the individual from whom it was obtained. Cartilage with low endogenous levels are stimulated by interleukin 1 (IL-1) to levels comparable to the high endogenous group. Total blood mononuclear cell products obtained from different individuals either behave in a manner similar to that seen with IL-1, which results in sustained high levels of enzyme secretion, or show an initial stimulation followed by a subsequent dropoff in enzyme secretion even though incubation is continued in the presence of mononuclear cell products. The factor(s) causing this dropoff can be distinguished from IL-1. Production and regulation of such factors may reflect a mechanism whereby the action of IL-1 can be controlled during the inflammatory response.
Campbell
I K
IK
Joint Diseases Laboratory, Shriners Hospital for Crippled Children, Montreal, PQ, Canada.
Guttman
A
A
Golds
E E
EE
Roughley
P J
PJ
Mort
J S
JS
D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000201:enzymology; D002358:Cartilage, Articular / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism; D002470:Cell Survival; D003470:Culture Media; D006801:Humans; D007206:Individuality; D007375:Interleukin-1 / Q000494:pharmacology; D007930:Leucine / Q000378:metabolism; D019278:Matrix Metalloproteinase 3; D008666:Metalloendopeptidases / Q000378:metabolism*; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D009994:Osmolar Concentration; D012016:Reference Values; D013997:Time Factors
D003470:Culture Media; D007375:Interleukin-1; D008666:Metalloendopeptidases; D019278:Matrix Metalloproteinase 3; D007930:Leucine