title: Effects of saikosaponin metabolites on the hemolysis of red blood cells and their adsorbability on the cell membrane.
abstract: The hemolytic properties and the adsorbability on red blood cells of saikosaponin a, saikosaponin d and 13 metabolites formed in the alimentary tract were investigated. Among these compounds, saikosaponin d and its intestinal product, prosaikogenin G, which possess an alpha-hydroxyl function at C16, showed the strongest hemolytic activity at the dose range of 1.0 to 5.0 micrograms/ml. Saikosaponin a and its intestinal product, prosaikogenin F, which possess a beta-hydroxyl function at C16, showed activity above 10 micrograms/ml. In this case, the monoglycoside, prosaikogenin F, showed the stronger activity than the diglycoside, saikosaponin a. Among the gastric products whose ether ring was cleaved to produce a carbinol, the monoglycosides, prosaikogenin A and prosaikogenin H, showed a slight activity above 25 micrograms/ml, and the saikogenins except saikogenin A were inactive. Saikogenin A, however, had hemolytic activity at a dose of 15 micrograms/ml. The adsorbabilities of these compounds on red blood cell membranes closely paralleled their degrees of hemolytic activity.
authors.forename: M
authors.initials: M
authors.lastname: Nose
authors.forename: S
authors.initials: S
authors.lastname: Amagaya
authors.forename: Y
authors.initials: Y
authors.lastname: Ogihara
mesh_terms: D000327:Adsorption; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D009828:Oleanolic Acid / Q000031:analogs & derivatives*; D012502:Sapogenins / Q000378:metabolism* / Q000494:pharmacology; D012503:Saponins
chemical_list: D012502:Sapogenins; D012503:Saponins; D009828:Oleanolic Acid; C025759:saikosaponin D