A preliminary study of brain gliomas with H17E2 monoclonal antibody: immunoscintigraphy and pharmacokinetics.
Ten patients with relapsing high grade brain gliomas and one patient with low grade glioma were studied with a monoclonal antibody (H17E2) against placental alkaline phosphatase. In addition 2 patients with relapsing high grade glioma were studied with a non specific antibody (4D513/2118). 1 mCi of Iodine-131-labelled H17E2 was administered intracarotidly (i.c.) in two, and intravenously (i.v.) in 9 patients. Immunoscintigrams were taken at 0, 2, 24, 48 and 72 hours. Radioactivity was monitored in blood and urine. Tumour/non-tumour ratios were estimated (max. 2.45). All high grade gliomas receiving specific antibody irrespective of the route of administration, gave a positive immunoscintigraphic pattern, increasing in intensity with time. Disappearance of radioactivity in blood was biexpontential with a long component over 30 hours. Urinary excretion of radioactivity ranged from 3.7-21.7% of administered dose/day. The patient with low grade glioma and the patients receiving non specific monoclonal antibody showed a negative pattern, a fast blood clearance and a high urinary excretion. We conclude that a) Iodine-131 labelled H17E2 proved to be stable in vivo and produced satisfactory tumour localisation and b) i.v. route was as good as i.c.
Skarlos
D V
DV
Agii Anargiri Cancer Hospital, Athens, Greece.
Malamitsi
J
J
Sivolapenko
G B
GB
Demakopoulos
N
N
Avgoustatos
G
G
Koutoulidis
C
C
Epenetos
A A
AA
D000469:Alkaline Phosphatase / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000008:administration & dosage; D014408:Biomarkers, Tumor / Q000276:immunology; D001932:Brain Neoplasms / Q000175:diagnosis* / Q000000981:diagnostic imaging / Q000276:immunology; D005910:Glioma / Q000175:diagnosis* / Q000000981:diagnostic imaging / Q000276:immunology; D006801:Humans; D007457:Iodine Radioisotopes; D007700:Kinetics; D011877:Radionuclide Imaging
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D007457:Iodine Radioisotopes; D000469:Alkaline Phosphatase