A novel isocratic HPLC method to separate and quantify acetanilide and its hydroxy aromatic derivatives: 2-, 3- and 4-hydroxyacetanilide (paracetamol or acetaminophen).
Reverse-phase high performance liquid chromatography on a microBondapak C-18 Column has been used to separate and quantify acetanilide and its aromatic monohydroxy derivatives in the 2-, 3- and 4- positions. Separation was achieved within 22 min by using an isocratic mixture of 2-propanol: methanol: water, 8:18:74 (v/v). This method compares very favourably with other HPLC techniques already reported to separate acetanilide from the monohydroxy aromatic derivatives.
Mancilla
J
J
Department of Biochemistry, Faculty of Medicine, University of Chile, Santiago.
Valdes
E
E
Gil
L
L
D000082:Acetaminophen / Q000302:isolation & purification*; D000083:Acetanilides / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet
D000083:Acetanilides; D012997:Solvents; D000082:Acetaminophen