A chemical modification approach to the olfactory code: vapor phase labeling using photoaffinity odorants.
A photoaffinity labeling technique was used to study the receptors involved in the discrimination of odorants. Aromatic azides, 1-azidonaphthalene (AzN) and 1-azido-4-nitronaphthalene (AsNN), were found to be pleasant-smelling compounds and produced good responses, giving standard EOG's (electro-olfactogram) of the kind observed for normal odorants. Following irradiation of the frog olfactory mucosa with light during constant stimulation with one of the azides vapor, there was a specific partial inhibition of the receptors for that odorant. The extent of reduction in amplitude of the EOG responses to AzN and AzNN varied between 40 to 60% of the original amplitude.
Menevşe
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Department of Medical Biology and Genetics, Faculty of Medicine, Gazi University, Ankara, Turkey.
Menevşe
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D000345:Affinity Labels; D000818:Animals; D001386:Azides / Q000494:pharmacology*; D004594:Electrophysiology; D009281:Naphthalenes / Q000494:pharmacology*; D009812:Odorants; D009831:Olfactory Mucosa / Q000187:drug effects / Q000502:physiology*; D011896:Rana temporaria; D012903:Smell / Q000187:drug effects / Q000502:physiology*
D000345:Affinity Labels; D001386:Azides; D009281:Naphthalenes