Preparation of monoclonal antibodies against okadaic acid prepared from the sponge Halichondria okadai.
Three murine monoclonal antibodies, OA-1, OA-2 and OA-3, against okadaic acid were prepared from hybridoma clones obtained by fusion of mouse 653 myeloma cells with mouse immune spleen cells sensitized to okadaic acid-ovalbumin conjugate. Each antibody reacted with dinophysistoxin-1 ( = 35-methylokadaic acid) as well as okadaic acid, but did not react with the other diarrhetic shellfish poisons or related compounds, such as 7-O-palmitoyl-okadaic acid (analogue of dinophysistoxin-3), pectenotoxin-1 and yessotoxin. A competitive inhibition enzymelinked immunosorbent assay which employed OA-3 antibody was performed and showed a sensitivity of about 10 ppb (10 ng/ml) for okadaic acid. This simple and time-saving ELISA assay system may be useful for the specific detection of diarrhetic shellfish poisons.
Usagawa
T
T
Ube Research Laboratory, Ube Industries Ltd., Japan.
Nishimura
M
M
Itoh
Y
Y
Uda
T
T
Yasumoto
T
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D003429:Cross Reactions / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D004988:Ethers, Cyclic / Q000276:immunology*; D005260:Female; D006825:Hybridomas; D008387:Marine Toxins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D019319:Okadaic Acid; D011161:Porifera / Q000032:analysis*; D013154:Spleen / Q000166:cytology; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D004988:Ethers, Cyclic; D008387:Marine Toxins; D019319:Okadaic Acid