title: Modulation of albumin secretion by ornithine alpha-ketoglutarate in adult rat hepatocyte cultures and a human hepatoma cell line (HepG2).
abstract: Cocultured adult rat hepatocytes and a human hepatoma cell line (HepG2) were maintained in an arginine-free medium with or without ornithine alpha-ketoglutarate. This drug increased greatly hepatocyte albumin secretion in both culture models. L-Ornithine was the component accounting for these effects since similar data were obtained by using this sole amino acid. Moreover, we observed that L-ornithine stimulation of albumin production was via polyamine synthesis. Since a correlated increase in albumin mRNA was observed, it may be postulated that ornithine alpha-ketoglutarate acts at a pretranslational level.
authors.affiliation: INSERM U 49, Unité de Recherche Hépatologique, Rennes, France.
authors.forename: G
authors.initials: G
authors.lastname: Lescoat
authors.forename: B
authors.initials: B
authors.lastname: Desvergne
authors.forename: O
authors.initials: O
authors.lastname: Loreal
authors.forename: N
authors.initials: N
authors.lastname: Pasdeloup
authors.forename: Y
authors.initials: Y
authors.lastname: Deugnier
authors.forename: M
authors.initials: M
authors.lastname: Bourel
authors.forename: P
authors.initials: P
authors.lastname: Brissot
mesh_terms: D000418:Albumins / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D006801:Humans; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology*; D009952:Ornithine / Q000031:analogs & derivatives* / Q000494:pharmacology; D051381:Rats
chemical_list: D000418:Albumins; C002118:ornithine alpha-ketoglutarate; D009952:Ornithine