A thin-layer multistrip polyacrylamide gel electrophoresis apparatus for Ferguson plot analysis at the submicrogram load level.
A procedure was developed for casting thin-layer multistrip polyacrylamide gels and using them for the simultaneous gel electrophoresis at several gel (Ferguson plot analysis) at the sub-microgram load level, using silver staining, autoradiography and, potentially, blotting for detection. The lower viscosity of polymerization mixtures, compared to agarose gelation mixtures, required the redesign of the multistrip cassette with separation of channels by rubber gaskets and the application of a cassette press. The lowered viscosity also required addition of 35% sucrose and an increased rate of polymerization in application to multistrip gels formed on a common NetFix backing. The present design allows one to obtain Ferguson plots exemplified by those of 32P-labeled DNA followed by autoradiographic detection.
Orbán
L
L
Section on Macromolecular Analysis, National Institute of Child Health and Human Development, Bethesda, MD 20892.
Sullivan
J V
JV
Zwieb
C
C
Chrambach
A
A
D004247:DNA; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation* / Q000379:methods; D008970:Molecular Weight
D004247:DNA