title: O-specific polysaccharides of Hafnia alvei lipopolysaccharides isolated from two serologically related strains: ATCC 13337 and 1187. A serological and structural study using chemical methods, gas chromatography/mass spectrometry and NMR spectroscopy at 500 MHz.
abstract: The O-specific polysaccharides of Hafnia alvei ATCC 13337 standard strain and 1187 strain have been isolated and characterized. By means of 1H-NMR spectroscopy, methylation analysis and periodate oxidation, the repeating unit of the polysaccharides could be allocated the respective structures. (formula; see text) where Acyl = D-3-hydroxybutyryl, and 3-O-acetylation was to about 66%. The structural similarity of the polysaccharides was confirmed in the serological study; their epitopes were determined and the importance of various structural elements for the serological specificity was discussed.
authors.affiliation: Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Wroclaw, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Gamian
authors.forename: E
authors.initials: E
authors.lastname: Romanowska
authors.forename: H J
authors.initials: HJ
authors.lastname: Opferkuch
authors.forename: M
authors.initials: M
authors.lastname: Hauck
authors.forename: J
authors.initials: J
authors.lastname: Dabrowski
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004755:Enterobacteriaceae / Q000276:immunology*; D008401:Gas Chromatography-Mass Spectrometry; D008070:Lipopolysaccharides; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000302:isolation & purification*
chemical_list: D008070:Lipopolysaccharides; D011134:Polysaccharides