[Gel chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis studies on urinary protein of mercury workers].
The results showed that in mankind, the urinary mercury consisted mainly of low molecular mercury complex (LM-Hg), low molecular protein-combining mercury (LMW-Pr-Hg) and high or middle molecular protein-combining mercury (HMMW-Pr-Hg) just like that found in animal experiments. The study demonstrated that the LM-Hg seemed to be helpful in refleting the detoxifying potentiality of renal tubular cell to wards mercury. The HMMW-Pr-Hg, made up mainly by albumin-combining mercury, was also a chief component of urinary mercury and showed parallel changes with the level of total urinary mercury. It suggested that the filtration through glomerulus was also an important source of urinary mercury, thus offering a new and completely different mercury excretion mechanism of kidney from classical conception. The results showed as well that the constitution of urinary protein in mercury workers might be of great variety, but the typical figure was the low molecular pattern. Because the changes in constitution of urinary protein were earlier than the changes in quantity, it was considered that the component analysis of urinary protein would be a more significant subclinical index.
Zhao
J Y
JY
D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006801:Humans; D008628:Mercury / Q000652:urine; D008630:Mercury Poisoning / Q000652:urine*; D009784:Occupational Diseases / Q000652:urine*; D011507:Proteinuria / Q000652:urine*
D008628:Mercury