[Immunochemical analysis of the products of translation of mRNA hybridized with the left HindIII-A-Sa1-fragment of vaccinia virus DNA].
The left HindIII-A-Sal fragment of the vaccinia virus DNA has been analyzed by the technique of mRNA hybridizational selection with the subsequent translation in cell-free protein-synthesizing system from the rabbit reticulocytes. The viral mRNA hybridizable with the fragment was shown to direct the synthesis of 12, 17, 27, 42, 70 kD polypeptides in the cell-free protein-synthesizing system. Each of 12 and 42 kD polypeptides was demonstrated to react specifically with antisera to structural p12 and p42 coat proteins. The structural coat proteins p12, p20, p42 of the vaccinia virus are concluded to be the products of the same viral gene.
Netesova
N A
NA
Muravlev
A I
AI
Chikaev
N A
NA
D000818:Animals; D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics*; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; D007120:Immunochemistry; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D015183:Restriction Mapping; D014616:Vaccinia virus / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000032:analysis*; D015678:Viral Structural Proteins / Q000032:analysis*
D004279:DNA, Viral; D012333:RNA, Messenger; D014759:Viral Envelope Proteins; D015678:Viral Structural Proteins; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; C029792:GTCGAC-specific type II deoxyribonucleases
Immunokhimicheskiĭ analiz produktov transliatsii mRNK, gibridiziruiushcheĭsia s levym HindIII-A-Sa1-fragmentom DNK virusa ospovaktsiny.