Preparative reversed-phase high-performance liquid chromatography in the synthesis of viscosin, a cyclic depsipeptide.
The importance of peptides in biochemical research and the facility with which they can be prepared by solid-phase techniques highlights the need for continuing research in the chromatographic purification of these molecules on a preparative scale. In this regard, synthesis of the cyclic depsipeptide antibiotic, viscosin, has provided the opportunity to demonstrate the use of radial compression cartridge technology in the reversed-phase purification of three key peptide intermediates on a scale of several hundred milligrams. Superior resolution of linear and cyclic peptide mixtures on a Bondapak C18 radial compression cartridge by using an aqueous acetonitrile solvent system containing 0.1% trifluoroacetic acid contributed significantly to the first total synthesis of viscosin, and demonstrates the applicability of this system to the purification of peptide mixtures.
Burke
T R
TR
Peptide Technologies Corporation, Washington, DC 20017.
Chandrasekhar
B
B
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D008969:Molecular Sequence Data; D010456:Peptides, Cyclic / Q000138:chemical synthesis / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
D010456:Peptides, Cyclic; C062796:viscosin