High performance liquid chromatographic (HPLC) determination of centchroman in human serum and application to single-dose pharmacokinetics.
A simple and sensitive (2 ng/ml) HPLC method with fluorescence detection has been developed to measure serum concentrations of centchroman, a new nonsteroidal antifertility agent. The method was sufficiently sensitive to follow the drug over 21 days in human volunteers. Pharmacokinetic parameters of centchroman were determined after a single oral dose of 60 mg (2 x 30-mg tablets) in two healthy female volunteers. Centchroman is slowly eliminated from serum, showing a biexponential disappearance curve from serum. The terminal half-life of centchroman in the two volunteers was 168 and 175 hr, respectively.
Paliwal
J K
JK
Pharmacokinetics and Metabolism Division, Central Drug Research Institute, Lucknow, India.
Gupta
R C
RC
Grover
P K
PK
Asthana
O P
OP
Srivastava
J S
JS
NityaNand
S
S
D000328:Adult; D001578:Benzopyrans / Q000097:blood*; D002486:Centchroman / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006207:Half-Life; D006801:Humans; D015203:Reproducibility of Results
D001578:Benzopyrans; D002486:Centchroman