title: Substrate recognition determinants for rhodopsin kinase: studies with synthetic peptides, polyanions, and polycations.
abstract: Rhodopsin kinase phosphorylates serine- and threonine-containing peptides from bovine rhodopsin's carboxyl-terminal sequence. Km's for the peptides decrease as the length of the peptide is increased over the range 12-31 amino acids, reaching 1.7 mM for peptide 318-348 from the rhodopsin sequence. The Km for phosphorylation of rhodopsin is about 10(3) lower than that for the peptides, which suggests that binding of rhodopsin kinase to its substrate, photolyzed rhodopsin, involves more than just binding to the carboxyl-terminal peptide region that is to be phosphorylated. A synthetic peptide from the rhodopsin sequence that contains both serines and threonines is improved as a substrate by substitution of serines for the threonines, suggesting that serine residues are preferred as substrates. Analogous 25 amino acid peptides from the human red or green cone visual pigment, a beta-adrenergic receptor, or M1 muscarinic acetylcholine receptors are better substrates for bovine rhodopsin kinase than is the peptide from bovine rhodopsin. An acidic serine-containing peptide from a non-receptor protein, alpha s1B-casein, is also a good substrate for rhodopsin kinase. However, many basic peptides that are substrates for other protein kinases--histone IIA, histone IIS, clupeine, salmine, and a neurofilament peptide--are not phosphorylated by rhodopsin kinase. Polycations such as spermine or spermidine are nonessential activators of phosphorylation of rhodopsin or its synthetic peptide 324-348. Polyanions such as poly(aspartic acid), dextran sulfate, or poly(adenylic acid) inhibit the kinase. Poly(L-aspartic acid) is a competitive inhibitor with respect to rhodopsin (KI = 300 microM) and shows mixed type inhibition with respect to ATP.
authors.affiliation: Department of Ophthalmology, University of Florida, Gainesville 32610.
authors.forename: K
authors.initials: K
authors.lastname: Palczewski
authors.forename: A
authors.initials: A
authors.lastname: Arendt
authors.forename: J H
authors.initials: JH
authors.lastname: McDowell
authors.forename: P A
authors.initials: PA
authors.lastname: Hargrave
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D003600:Cytosol / Q000378:metabolism; D005136:Eye Proteins; D051606:G-Protein-Coupled Receptor Kinase 1; D008969:Molecular Sequence Data; D010766:Phosphorylation; D010786:Photoreceptor Cells / Q000201:enzymology*; D011073:Polyamines; D000071228:Polyelectrolytes; D011108:Polymers / Q000494:pharmacology*; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000378:metabolism*; D012243:Rhodopsin / Q000378:metabolism; D012374:Rod Cell Outer Segment / Q000201:enzymology*; D013379:Substrate Specificity / Q000187:drug effects
chemical_list: D005136:Eye Proteins; D051606:G-Protein-Coupled Receptor Kinase 1; D011073:Polyamines; D000071228:Polyelectrolytes; D011108:Polymers; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases; D012243:Rhodopsin