[Analysis of structural proteins and products of cell-free translation of vaccinia virus of mRNA using mono-specific antibodies].
The monospecific antiserums to the major proteins p12, p19, p20, p42, p61 of the vaccinia virus coat were obtained and analyzed. The dynamics of the proteins accumulation in the infected cells has been studied. Products of the cell-free translation of the total viral mRNA precipitated by the obtained antiserums were identified. The p20 protein has been found to be a result of p12 protein reversible oligomerization under the conditions of electrophoresis. The antigenic relation of p12 and p20 proteins to a p42 protein, also a p12 oligomer, has been demonstrated. The possibility is discussed to use the obtained antiserums for mapping of the genes corresponding to structural proteins in the genome of the vaccinia virus.
Muravlev
A I
AI
Netesova
N A
NA
Tikhonov
V Ia
VIa
Malygin
E G
EG
D000818:Animals; D015152:Blotting, Northern; D002213:Capsid / Q000235:genetics*; D002474:Cell-Free System; D005814:Genes, Viral; D007106:Immune Sera; D007150:Immunohistochemistry; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D011817:Rabbits; D011863:Radioimmunoassay; D014616:Vaccinia virus / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics
D007106:Immune Sera; D012333:RNA, Messenger; D015678:Viral Structural Proteins
Analiz strukturnykh belkov i produktov beskletochnoĭ transliatsii mRNKvirusa ospovaktsiny s pomoshch'iu monospetsificheskikh antisyvorotok.