Heat-labile serum inhibitor of anti-phospholipid antibody binding in ELISA.
Normal human sera (NHS), heat-inactivated at 56 degrees C for 30 min, demonstrated positive ELISA reactions for anti-cardiolipin (aCL) antibodies. The heat-induced reactivity in ELISA was inhibitable by the cardiolipin antigen and was abolished by prior IgG depletion of the heated NHS with a protein A preparation. The heat-potentiated aCL also cross-reacted selectively with phosphatidic acid and phosphatidylserine, but not with phosphatidylcholine or phosphatidylethanolamine.
Cheng
H M
HM
Department of Physiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Phuah
E B
EB
D001685:Biological Factors / Q000097:blood* / Q000276:immunology; D002308:Cardiolipins / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006358:Hot Temperature; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D010743:Phospholipids / Q000276:immunology*
D001685:Biological Factors; D002308:Cardiolipins; D007074:Immunoglobulin G; D010743:Phospholipids