In vitro effects of cadmium and arsenite on glutathione peroxidase, aspartate and alanine aminotransferases, cholinesterase and glucose-6-phosphate dehydrogenase activities in blood.
The activity of several blood enzymes in the presence and absence of arsenite (As) and cadmium (Cd) was investigated under in vitro conditions. Both human and rat blood glutathione peroxidase (GSH-Px) activities were adversely affected by As at the 0.8 and 1.6 micrograms/ml levels. The latter was completely inactivated whereas the former retained approximately 30% of its original activity. The effect of Cd on this enzyme was much smaller: 650 g Cd/ml were needed to decrease its activity by 30% of the original value. As noted for GSH-Px, the rat's glutamyl oxaloacetate transaminase (GOT) appears to be appreciably more sensitive to the As inhibitory effect than the human enzyme (by a factor of 3). Cd, however, failed to bring about any inhibition of GOT. In the case of glutamyl pyruvate transaminase (GPT) both As and Cd had a marked effect, manifested in 70% and 78% inhibition, respectively. Blood glucose-6-phosphate dehydrogenase (G-G-PD) was inhibited by both Cd and As, however, within the concentration range used, only Cd inhibited it completely. Cholinesterase (ChE) activity was inhibited completely by both Cd and As.
Sheabar
F Z
FZ
Department of Food Engineering and Biotechnology, Technion-Israel Institute of Technology, Haifa.
Yannai
S
S
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001151:Arsenic / Q000633:toxicity*; D018053:Arsenites; D001219:Aspartate Aminotransferases / Q000097:blood; D002104:Cadmium / Q000633:toxicity*; D002800:Cholinesterase Inhibitors; D002802:Cholinesterases / Q000097:blood*; D005954:Glucosephosphate Dehydrogenase / Q000097:blood*; D005979:Glutathione Peroxidase / Q000037:antagonists & inhibitors / Q000097:blood*; D006801:Humans; D066298:In Vitro Techniques; D051381:Rats; D000637:Transaminases / Q000097:blood*
D018053:Arsenites; D002800:Cholinesterase Inhibitors; D002104:Cadmium; D005954:Glucosephosphate Dehydrogenase; D005979:Glutathione Peroxidase; D000637:Transaminases; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D002802:Cholinesterases; C015001:arsenite; D001151:Arsenic