Determination of propranolol and 4-hydroxypropranolol in human plasma by high-performance liquid chromatography.
A high-performance liquid chromatographic method was developed to determine the levels of propranolol and its major metabolite, 4-hydroxypropranolol, in human plasma. The limits of determination are 10 ng ml-1 of propranolol and 5 ng ml-1 of 4-hydroxypropranolol using a 0.5-ml plasma sample. The stability of plasma samples stored at -30 degrees C for up to 2 months was also tested. No stabilising antioxidants were added to the samples.
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D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D011433:Propranolol / Q000031:analogs & derivatives* / Q000097:blood*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
C001422:4-hydroxypropranolol; D011433:Propranolol