title: [Isolation and purification of biopolymers using an affinity chromatography method. IX. Preparation, properties and use of affinity adsorbents with immobilized polypeptide fragments of collagen].
abstract: Synthesis and properties of new affinity adsorbents with immobilized polypeptide fragments of collagen molecule (alpha-chains, beta-components, cyanogen bromide peptides) were described. Adsorbents with alpha-chains and alpha 1CB7-peptide had fibronectin binding capacity 1.5-2.0 times higher than commercial gelatin-Sepharose. Commercial production of highly purified fibronectin from human plasma using affinity chromatography on immobilized individual alpha-chains of collagen was developed.
authors.forename: V Kh
authors.initials: VKh
authors.lastname: Mitina
authors.forename: N A
authors.initials: NA
authors.lastname: Frantsuzova
authors.forename: V A
authors.initials: VA
authors.lastname: Kliashitskiĭ
authors.forename: Iu M
authors.initials: IuM
authors.lastname: Krasnopol'skiĭ
authors.forename: G A
authors.initials: GA
authors.lastname: Sennikov
authors.forename: V I
authors.initials: VI
authors.lastname: Svets
authors.forename: V N
authors.initials: VN
authors.lastname: Orekhovich
mesh_terms: D000818:Animals; D001704:Biopolymers; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003094:Collagen; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D046911:Macromolecular Substances; D010446:Peptide Fragments; D051381:Rats; D013056:Spectrophotometry, Ultraviolet
chemical_list: D001704:Biopolymers; D005353:Fibronectins; D046911:Macromolecular Substances; D010446:Peptide Fragments; D003094:Collagen
vernacular_title: Vydelenie i ochistka biopolimerov metodom affinnoĭ khromatografii. IX. Poluchenie, svoĭstva i primenenie affinnykh adsorbentov s immobilizovannymi polipeptidnymi fragmentami kollagena.