Influence of immunosuppressants against Dirofilaria immitis infection in dogs.
Two immunosuppressive drugs, azathiopurine (AZP) and prednisolone (PDS), were examined on dogs experimentally infected with Dirofilaria immitis in order to estimate the involvement of immunological assail in rejecting the parasite by the host. AZP was orally administered to 3 dogs daily at a dosage of 1-10 mg/kg for a period from 3 days before infection until the end of the experiment. The dose was then varied and transiently ceased according to the severity of the side effects. PDS was subcutaneously administered daily to 2 dogs. They were administered 10 mg/kg of PDS from 3 days before infection to day 15 and 5.0-8.5 mg/kg from day 60 after infection to day 70. The serum D. immitis-specific antibody level assessed by an indirect hemagglutination test was steadily decreased in the AZP-medicated dogs. However, in the PDS-medicated dogs, the antibody titer was decreased until day 32 and, thereafter, was recovered. When all dogs were sacrificed between days 145-148, an average recovery rate of worms in both the AZP- and PDS-medicated dogs was 52.5% and 49.6%, respectively, while the controls showed 42.5%. The statistical analysis revealed no significant difference among the groups, indicating that the administration of AZP and PDS was not effective in protecting the larvae from the host's immune attack.
Hayasaki
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M
Ohishi
I
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D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis; D001379:Azathioprine / Q000494:pharmacology; D004183:Dirofilaria immitis / Q000187:drug effects / Q000276:immunology* / Q000302:isolation & purification; D004184:Dirofilariasis / Q000276:immunology / Q000662:veterinary*; D004283:Dog Diseases / Q000276:immunology*; D004285:Dogs; D005260:Female; D005370:Filarioidea / Q000276:immunology*; D006790:Host-Parasite Interactions; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008297:Male; D011239:Prednisolone / Q000494:pharmacology
D000909:Antibodies, Helminth; D007166:Immunosuppressive Agents; D011239:Prednisolone; D001379:Azathioprine