Bovine interleukin 2: biochemical and biological characterization.
Interleukin 2 (IL-2), secreted by bovine peripheral blood mononuclear cells (PBL) on stimulation with concanavalin A (Con A), was purified and characterized by different chromatographic and electrophoretic techniques. The ability of IL-2 to support proliferation of Con A-stimulated bovine lymphoblasts was used to assay and quantitate IL-2 activity. Bovine IL-2 having an apparent MW of 27,000 eluted from a gel-filtration column; from an anion exchange column peak activity was detected at 190 mM NaCl. Binding of bovine IL-2 to phenyl-Sepharose gel and elution with 35-60% ethanediol indicated its hydrophobic nature. Studies on cross-species reactivity revealed that both buffalo and goat lymphocytes respond to cattle IL-2 and detected 35% of activity from a standard cattle IL-2 preparation. Sheep lymphocyte response to cattle IL-2 was negligible.
Arunachalam
B
B
Immunology Section, Indian Veterinary Research Institute, Izatnagar.
Subba Rao
M V
MV
Ram
G C
GC
D000818:Animals; D002020:Buffaloes; D002417:Cattle / Q000276:immunology*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003208:Concanavalin A / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006041:Goats; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000302:isolation & purification* / Q000494:pharmacology; D008213:Lymphocyte Activation; D012756:Sheep; D013045:Species Specificity
D007376:Interleukin-2; D003208:Concanavalin A