title: Identification and simultaneous determination of non-steroidal anti-inflammatory drugs using high-performance liquid chromatography.
abstract: An isocratic high-performance liquid chromatographic procedure is presented for the screening of plasma samples for the presence of sixteen non-steroidal anti-inflammatory drugs. Detection was achieved simultaneously at two wavelengths (254 and 370 nm) and the purity of the eluted peaks was tested using absorbance ratios at the two wavelengths; identification could thus be effective without interferences from substances of other pharmacological classes. The drugs were extracted simultaneously with diethyl ether after acidification and separated from each other on an octadecyl reversed-phase column using only one eluent, acetonitrile-0.3% acetic acid-tetrahydrofuran (36:63.1:0.9, v/v). The recovery, precision and reproducibility of the method were satisfactory as it allowed the determination of the drugs from infra- to supratherapeutic concentrations.
authors.affiliation: Département de Pharmacologie Clinique, Faculté de Médecine, Vandoeuvre les Nancy, France.
authors.forename: F
authors.initials: F
authors.lastname: Lapicque
authors.forename: P
authors.initials: P
authors.lastname: Netter
authors.forename: B
authors.initials: B
authors.lastname: Bannwarth
authors.forename: P
authors.initials: P
authors.lastname: Trechot
authors.forename: P
authors.initials: P
authors.lastname: Gillet
authors.forename: H
authors.initials: H
authors.lastname: Lambert
authors.forename: R J
authors.initials: RJ
authors.lastname: Royer
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006863:Hydrogen-Ion Concentration; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal