Lyophilization of rumen fluid for use in culture media.
The supernatant from centrifugation at 1,000 x g of strained rumen fluid was lyophilized, and the residue and sublimate fractions were used to replace fresh rumen fluid in a complete roll tube medium for enumeration of total rumen bacteria. Most of the growth-supporting nutrients in fresh rumen fluid were found in the residue fraction. With one exception, no significant differences were found in total bacterial numbers either by roll tube or most-probable-number procedures when lyophilized rumen fluid residue was substituted for fresh rumen fluid. Lyophilized rumen fluid residue was stable for at least 5 months at room temperature. Rumen fluid supernatant from centrifugation at 1,000 x g had a mean density of 1.005 +/- 0.03 g/ml and contained 1.56% +/- 0.30% dry matter. On the basis of these values, 15.68 mg of lyophilized rumen fluid residue is equivalent to 1 ml of rumen fluid supernatant from centrifugation at 1,000 x g.
Dehority
B A
BA
Department of Animal Science, Ohio Agricultural Research and Development Center, Ohio State University, Wooster 44691.
Tirabasso
P A
PA
D000818:Animals; D001419:Bacteria / Q000254:growth & development*; D002498:Centrifugation; D015169:Colony Count, Microbial; D003470:Culture Media; D005612:Freeze Drying; D012417:Rumen / Q000382:microbiology*
D003470:Culture Media