title: [ELISA method for the determination of factor VII antigen].
abstract: The low plasma concentration of clotting factor VII makes it difficult to assay its antigenic fraction by the conventional methods of precipitation with specific antigens. Simple and peroxidase-conjugated antisera are currently available from commercial sources, thus allowing one to determine F VII:Ag by enzyme immunoassay. An ELISA method has been developed in this laboratory which provides sensitivity limits about 0.1% of the plasma concentration of F VII and correlates significantly with its functional activity (r = 0.603, n = 44, p less than 0.001). This technique can be highly helpful in characterising molecular variants of F VII, as well as in detecting acquired deficiencies of this factor.
authors.forename: J I
authors.initials: JI
authors.lastname: Jorquera
authors.forename: J A
authors.initials: JA
authors.lastname: Aznar
authors.forename: J
authors.initials: J
authors.lastname: Monteagudo
authors.forename: J M
authors.initials: JM
authors.lastname: Montoro
authors.forename: P
authors.initials: P
authors.lastname: Casaña
authors.forename: I
authors.initials: I
authors.lastname: Pascual
authors.forename: E
authors.initials: E
authors.lastname: Bañuls
authors.forename: R
authors.initials: R
authors.lastname: Curats
authors.forename: F
authors.initials: F
authors.lastname: Llopis
mesh_terms: D000941:Antigens / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005167:Factor VII / Q000032:analysis / Q000276:immunology*; D006801:Humans
chemical_list: D000941:Antigens; C042580:factor VII clotting antigen; D005167:Factor VII
vernacular_title: Método ELISA para la determinación del antígeno del factor VII.