Gene mapping by enzymatic amplification from flow-sorted chromosomes.
A new approach to gene mapping which combines enzymatic amplification with high-resolution flow sorting of human chromosomes has been devised. Reliable amplification from as few as 200 chromosomes has been demonstrated. This method, with particular application to mapping the position of chromosomal translocations, has been used to show that the breakpoint for the constitutional translocation t(11;22)(q23;q11) lies proximal to the genes c-ets-1, Thy-1, and T3 delta and distal to the int-2 gene. The mapping was confirmed by Southern analysis to much larger numbers of chromosomes sorted from the same cell line. Control reactions for the bcl-2 gene on chromosome 18 and the C alpha gene of the IGH locus on chromosome 14 demonstrated the discrimination which can be achieved.
Cotter
F
F
ICRF Medical Oncology Laboratory, St. Bartholomew's Hospital, London, United Kingdom.
Nasipuri
S
S
Lam
G
G
Young
B D
BD
D001483:Base Sequence; D015139:Blotting, Southern; D002460:Cell Line; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics; D005434:Flow Cytometry; D005784:Gene Amplification; D005803:Genes, Immunoglobulin; D006801:Humans; D007621:Karyotyping; D014178:Translocation, Genetic
D004247:DNA