Resident macrophages specifically inhibit DNA synthesis in the nuclei of transformed cells in heterokaryons.
DNA synthesis in heterokaryons of mouse peritoneal macrophages and various proliferating cultured cells was analyzed. Macrophages did not inhibit replication in the nuclei of non-immortalized and spontaneously immortalized cells (rat fibroblasts and macrophages, mouse pre-macrophages, NIH3T3 cells). On the contrary, the percentage of DNA-synthesizing nuclei of malignant HeLa cells was drastically reduced in heterokaryons. The transformation of NIH3T3 cells with c-Ki-ras oncogene and that of rat chondrocytes with p53 oncogene made these cells sensitive to the replication-inhibiting activity of macrophages in heterokaryons. Our observation represents a kind of "intracellular" cytotoxic activity of macrophages directed against transformed cells.
Prudovsky
I A
IA
Engelhardt Institute of Molecular Biology, USSR Academy of Sciences, Moscow.
Gumeniuk
R R
RR
Yegorov
Y E
YE
Zelenin
A V
AV
D000818:Animals; D002455:Cell Division; D002459:Cell Fusion; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D004247:DNA / Q000096:biosynthesis; D006367:HeLa Cells; D066298:In Vitro Techniques; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D008808:Mice, Inbred CBA; D051381:Rats
D004247:DNA