title: Detection of apramycin resistant Enterobacteriaceae in hospital isolates.
abstract: Apramycin is a recently developed aminoglycoside restricted to veterinary therapy. Production of a 3-aminoglycoside acetyltransferase type IV (AAC(3)-IV) conferring cross-resistance to this drug and to gentamicin was detected in 1984 in France in bacteria of bovine origin. This mechanism of resistance was apparently confined to animals. We have studied 17 strains resistant to apramycin and gentamicin isolated in 5 hospitals in Belgium. Conjugative plasmids encoding an AAC(3)IV were present in 14 isolates. Comparison of the restriction fingerprints revealed 6 different plasmid patterns: 8 plasmids belonged to 2 groups sharing extensive intragroup homology and 4 were not related to the other replicons. These results indicate dissemination of plasmids within and between hospitals, but also of the gene encoding an AAC(3)IV.
authors.affiliation: Institut National de la Recherche Agronomique, Centre de Tours-Nouzilly, Monnaie, France.
authors.forename: E
authors.initials: E
authors.lastname: Chaslus-Dancla
authors.forename: Y
authors.initials: Y
authors.lastname: Glupcznski
authors.forename: G
authors.initials: G
authors.lastname: Gerbaud
authors.forename: M
authors.initials: M
authors.lastname: Lagorce
authors.forename: J P
authors.initials: JP
authors.lastname: Lafont
authors.forename: P
authors.initials: P
authors.lastname: Courvalin
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002417:Cattle; D003428:Cross Infection / Q000382:microbiology; D015342:DNA Probes; D004352:Drug Resistance, Microbial / Q000235:genetics; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000382:microbiology; D006801:Humans; D009328:Nebramycin / Q000031:analogs & derivatives / Q000494:pharmacology*; D009693:Nucleic Acid Hybridization; D011815:R Factors; D012093:Replicon; D014162:Transfection
chemical_list: D000900:Anti-Bacterial Agents; D015342:DNA Probes; D009328:Nebramycin; C011666:apramycin