Bioassay for determination of human serum luteinizing hormone (LH): a routine clinical method.
Serum levels of LH are used as marker of a number of pathological conditions. In the past many methods (RIA, IRMA, ELISA) have been employed to measure serum LH, these procedures, however, suffer from the drawback that they determine the immuno reactive and not the bioactive part of the hormone. An improved in vitro bioassay method for the estimation of serum LH has been described. The underlying mechanism of the assay is testosterone production by mouse Leydig cells in the presence of added LH. The method has been significantly improved in terms of sensitivity (0.2 IU/l) and simplicity; the assay is simple and does not require any special instruments and can be set up in any endocrinological laboratory.
Stadler
U
U
Zoological Institute, University of Salzburg, Austria.
Rovan
E
E
Aulitzky
W
W
Frick
J
J
Adam
H
H
Kalla
N
N
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D006801:Humans; D007985:Leydig Cells / Q000187:drug effects* / Q000378:metabolism; D007986:Luteinizing Hormone / Q000097:blood* / Q000494:pharmacology; D008297:Male; D051379:Mice; D011863:Radioimmunoassay; D012015:Reference Standards
D007986:Luteinizing Hormone