Purification of IgG from serum with caprylic acid and ammonium sulphate precipitation is not superior to ammonium sulphate precipitation alone.
Immunoglobulin G (IgG) from bovine serum raised against Aeromonas Salmonicida was purified by ammonium sulphate precipitation (ASP) or caprylic acid treatment followed by ammonium sulphate precipitation (CAAS). Purity of IgG samples prepared by both methods were examined by High Performance Gel Permeation Chromatography, electrophoresis and antibody activity assay. Results suggest that IgG prepared by ASP is better than that obtained by CAAS method in terms of the yield of the IgG monomers and the recovery of the antibody activity.
Mohanty
J G
JG
Department of Pathology and Microbiology, University of Montreal, St Hyacinthe, Quebec, Canada.
Elazhary
Y
Y
D000333:Aeromonas / Q000276:immunology*; D000372:Agglutination Tests; D000645:Ammonium Sulfate; D000818:Animals; D000907:Antibodies, Bacterial / Q000302:isolation & purification; D002210:Caprylates; D002417:Cattle; D011232:Chemical Precipitation; D002851:Chromatography, High Pressure Liquid; D007074:Immunoglobulin G / Q000302:isolation & purification*
D000907:Antibodies, Bacterial; D002210:Caprylates; D007074:Immunoglobulin G; D000645:Ammonium Sulfate