[Production and application of anti-human platelet GP IIb/IIIa monoclonal antibodies].
Four types of anti-human platelet glycoprotein IIb/IIIa (GP IIb/IIIa)monoclonal antibodies (McAb), termed HIP2, HIP4, HIP7, HIP8, were obtained in our laboratory. All of them recognized GP IIb/IIIa complex except that HIP2 recognized GP IIb. As to the effect of the four McAbs on platelet aggregation, the results showed: 1) HIP4 and HIP8 can completely inhibit platelet aggregation induced by ADP or collagen, but not that induced by thrombin or ristocetin. 2) HIP2 was able to activate platelet aggregation directly. But it is Ca++ and complement dependent; 3) HIP7 did not show any effect on platelet aggregation induced by collagen, ADP, thrombin or ristocetin. When clinically studied, some cases of platelet-associated diseases such as thrombocytasthenia and thrombocytopenia had abnormal responses to the above McAbs. In addition, we also found that these McAbs can stimulate hematopoiesis in CFU-E assay.
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D006801:Humans; D051379:Mice; D010974:Platelet Aggregation; D010980:Platelet Membrane Glycoproteins / Q000276:immunology*; D011696:Purpura, Thrombocytopenic / Q000175:diagnosis
D000911:Antibodies, Monoclonal; D010980:Platelet Membrane Glycoproteins