title: Rapid high-performance liquid chromatographic methods for the determination of overdose concentrations of some non-steroidal anti-inflammatory drugs in plasma or serum.
abstract: Separate methods are described for the determination of the non-steroidal anti-inflammatory drugs diflunisal, indomethacin, fenoprofen, ibuprofen, ketoprofen, naproxen, mefenamic acid and piroxicam at overdose concentrations in human plasma or serum, using high-performance liquid chromatography and ultraviolet detection. A common extraction, involving protein precipitation with acetonitrile, is employed for all methods. A 25 cm Hypersil ODS (5 mu particle size) analytical column is used for all chromatographic separations, with a mobile phase of acetonitrile-acetate buffer (pH 4.2 or 4.8). The methods are all reproducible and can also determine concentrations that fall within the normal therapeutic range for each drug.
authors.affiliation: Poisons Unit, Guys Hospital, London, U.K.
authors.forename: P J
authors.initials: PJ
authors.lastname: Streete
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood* / Q000506:poisoning; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal