Molecular species composition of the major phospholipids in brain and retina from rainbow trout (Salmo gairdneri). Occurrence of high levels of di-(n-3)polyunsaturated fatty acid species.
The molecular-species compositions of the diacyl classes of the major phospholipids from the brain and retina of rainbow trout (Salmo gairdneri) were determined. A total of 46 possible species was identified. Didocosahexaenoyl species were major components of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylserine (PS) from retina, comprising 14.1, 41.3 and 28.3% of the respective totals. This species was also abundant in PE and PS from brain, accounting for 14.9 and 19.9% of the totals respectively. Small amounts of di-polyunsaturated fatty acid species [C22:6(n-3) with C20:5(n-3), and C22:6(n-3) with C22:5(n-3)] were also found in these phospholipids. Phosphatidylinositol (PI) from both tissues contained no di-polyunsaturated fatty acid species. Retinal PI contained 40.1% C18:0-C20:4(n-6) with 14.9% of C18:0-C20:5(n-3); brain PI contained 42.3% of C18:0-C20:5 and 10.4% of C18:0-C20:4 species. Brain PC contained a substantial amount of nervonic acid-containing species with the pair C18:1-C24:1/C24:1-C18:1 comprising 8.9% of the total.
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NERC Unit of Aquatic Biochemistry, School of Molecular and Biological Sciences, University of Stirling, Scotland, U.K.
Tocher
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D000818:Animals; D001923:Brain Chemistry; D002855:Chromatography, Thin Layer / Q000379:methods; D004075:Diglycerides / Q000032:analysis / Q000138:chemical synthesis; D005227:Fatty Acids / Q000032:analysis; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D007202:Indicators and Reagents; D009928:Organ Specificity; D010743:Phospholipids / Q000302:isolation & purification*; D012160:Retina / Q000032:analysis*; D014280:Triglycerides / Q000302:isolation & purification; D014337:Trout
D004075:Diglycerides; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D007202:Indicators and Reagents; D010743:Phospholipids; D014280:Triglycerides