Reversed-phase liquid chromatographic retention and selectivity surfaces. II. Deoxyribonucleosides.
By plotting capacity factors and selectivity factors as functions of temperature and pH or methanol concentration of the mobile phase, retention and selectivity surfaces of four deoxyribonucleosides were generated. Although changes in the methanol content had little effect on selectivity, changes in pH and temperature could be used to improve selectivity. However, changes in pH or temperature can be coupled with changes in methanol concentration to optimize the analysis time. In addition, by using lines of constant analysis time (isochrons), it was found that several sets of conditions will result in nearly identical retention times. These isochrons can be used to optimize the resolution of the solutes.
Grushka
E
E
Department of Inorganic and Analytical Chemistry, Hebrew University, Jerusalem, Israel.
Jang
N I
NI
Brown
P R
PR
D002853:Chromatography, Liquid / Q000379:methods*; D003853:Deoxyribonucleosides / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D000432:Methanol / Q000032:analysis / Q000494:pharmacology; D013696:Temperature
D003853:Deoxyribonucleosides; D000432:Methanol