Molecular cloning, nucleotide sequence and fine-structural analysis of the Corynebacterium glutamicum fda gene: structural comparison of C. glutamicum fructose-1,6-biphosphate aldolase to class I and class II aldolases.
The Corynebacterium glutamicum fda gene encoding fructose-1,6-biphosphate (FBP) aldolase has been isolated by complementation of an Escherichia coli mutant. The nucleotide sequence of a 3371 bp chromosomal fragment containing the C. glutamicum fda gene was determined. The N-terminal amino acid sequence of C. glutamicum FBP aldolase identified the correct initiation site for the fda gene, and a molecular weight of 37,092 was predicted for the fda polypeptide. S1 nuclease mapping identified the transcriptional start site, and Northern hybridization analysis indicated that the fda gene encodes a single 1.3 kb transcript. The primary structure of C. glutamicum FBP aldolase shows strong homology to class II FBP aldolases. Conservation of primary structure was observed between class I and class II aldolases, but several residues essential for catalytic activity in class I aldolases were absent from class II aldolases.
von der Osten
C H
CH
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Barbas
C F
CF
Wong
C H
CH
Sinskey
A J
AJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003352:Corynebacterium / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D005634:Fructose-Bisphosphate Aldolase / Q000145:classification / Q000235:genetics*; D005798:Genes, Bacterial; D005947:Glucose; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D014158:Transcription, Genetic
D004269:DNA, Bacterial; D005634:Fructose-Bisphosphate Aldolase; D005947:Glucose