Tissue factor gene expression in acute myeloblastic leukemia.
We have studied tissue factor gene expression in the leukocytes of 22 patients with acute myeloblastic leukemia (AML). Total RNA from peripheral blood or bone marrow cells depleted of monocytes was analyzed by Northern blot analysis using a 32P-labeled tissue factor cDNA probe. Cells from 10 cases expressed tissue factor mRNA and positive cases were distributed among the myeloblastic, myelomonocytic, and monocytic subtypes of AML. Tissue factor transcripts were not detected in cells derived from normal bone marrow. The expression of this gene product on the surface of leukemic cells could contribute to the excessive thrombin generation that has been observed in some individuals with this disorder.
Bauer
K A
KA
Charles A. Dana Research Institute, Boston, MA.
Conway
E M
EM
Bach
R
R
Konigsberg
W H
WH
Griffin
J D
JD
Demetri
G
G
D001779:Blood Coagulation Factors / Q000032:analysis*; D001853:Bone Marrow / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000097:blood / Q000235:genetics*; D015479:Leukemia, Myelomonocytic, Acute / Q000097:blood / Q000235:genetics*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis; D013925:Thromboplastin / Q000096:biosynthesis* / Q000235:genetics
D001779:Blood Coagulation Factors; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; C045071:leukocyte procoagulant activity; D013925:Thromboplastin