[A phased poly-linker for cloning and expression of genes].
15 oligodeoxynucleotides were synthesized using the phosphotriether technique which were subsequently enzymatically ligated in polylinker with subsequent phasing of left and right sides. Based on the phased polylinker a series of vehicles for the gene cloning and expression was constructed. The vectors of pRK series contain all three variants of polylinker with the frame shift of the reading frame for 1, 2, or 3 nucleotides in both chains. The obtained polylinkers do not effect the enzymatic activity of the beta-galactosidase alpha-peptide. Structure of the phased polylinker was confirmed by Maxam and Gilbert's sequencing method.
Karpov
V A
VA
Lunin
V G
VG
Tikhonenko
T I
TI
D000595:Amino Acid Sequence; D001483:Base Sequence; D002852:Chromatography, Ion Exchange; D003001:Cloning, Molecular; D004587:Electrophoresis, Agar Gel; D015870:Gene Expression; D008969:Molecular Sequence Data; D010957:Plasmids; D015183:Restriction Mapping
Faziruiushchiĭ polilinker dliia klonirovaniia i ékspressii genov.