[Complex experimental assessment of potential allergenicity of various isolates of soy proteins].
The complex evaluation was carried out of potential allergenicity of three soya protein specimens - IPS, IPS-1 and IPS-2 manufactured by Ardex R, USA, which were attended for employment as protein component of specialized infant formulae. The content of total protein, amino-nitrogen, peptide fractions of molecular weight below 9000 D, precipitating protein antigens, trypsin inhibitor, was studied in the specimens. The apparent digestibility was measured by a multienzymatic system in vitro. The allergenicity of isolates was compared by means of histamine LD50 resistance test in guinea pigs and also by the reaction of active anaphylactic shock (AAS). The evidences have been obtained that IPS specimen containing the lowest levels of trypsin inhibitor and small peptide levels is characterized by the lowest sensitizing activity in AAS and simultaneously decreases the animals' resistance to histamine in the highest degree. Apparent digestibility of all the three specimens did not differ significantly. A conclusion has been made that the evaluation is necessary of infant hypoallergenic formulae components by a complex of independent experimental data.
Mazo
V K
VK
Samenkova
N F
NF
Gmoshinskiĭ
I V
IV
Pepeliaev
Iu V
IuV
Zorin
S N
SN
Krzhechkovskaia
V V
VV
Malikova
N A
NA
Marokko
I N
IN
Ushakov
V V
VV
D000818:Animals; D002850:Chromatography, Gel; D005512:Food Hypersensitivity / Q000209:etiology*; D006168:Guinea Pigs; D007122:Immunoelectrophoresis; D007225:Infant Food / Q000009:adverse effects* / Q000032:analysis; D008297:Male; D014674:Plant Proteins, Dietary / Q000009:adverse effects* / Q000032:analysis; D030262:Soybean Proteins
D014674:Plant Proteins, Dietary; D030262:Soybean Proteins
Kompleksnaia éksperimental'naia otsenka potentsial'noĭ allergennosti nekotorykh obraztsov izoliatov soevykh belkov.