title: Identification of an androgen receptor in the adult chicken oviduct.
abstract: The chicken oviduct androgen receptor was characterized by sucrose density gradient centrifugation, Scatchard analysis, competition studies, and affinity labeled with dihydrotestosterone 17 beta-bromoacetate. A specific 8.5 S peak was seen on 0.01 M KCl sucrose density gradients when the receptor was labeled with [3H]5 alpha-dihydrotestosterone. Specific 4.6 S peaks were seen when receptor labeled with [3H]5 alpha-dihydrotestosterone or [3H]dihydrotestosterone 17 beta-bromoacetate was analyzed on 0.3 M KCl sucrose density gradients. Scatchard analysis of [3H]5 alpha-dihydrotestosterone binding by oviduct cytosol was consistent with two binding sites. A Kd of 0.13 nM was found for the high affinity androgen receptor. Competition studies showed the following order of ligand affinity: 5 alpha-dihydrotestosterone greater than dihydrotestosterone 17 beta-bromoacetate greater than progesterone greater than estradiol. A 61.2 kDa protein was specifically covalently labeled with [3H]dihydrotestosterone 17 beta-bromoacetate. The chicken oviduct androgen receptor possesses characteristics similar to other androgen receptors, and provides a good source of androgen receptor for physicochemical studies of the native receptor protein.
authors.affiliation: Department of Medicine, Vanderbilt University Medical Center, Nashville, TN.
authors.forename: D L
authors.initials: DL
authors.lastname: Ellis
authors.forename: B J
authors.initials: BJ
authors.lastname: Danzo
mesh_terms: D000345:Affinity Labels; D000818:Animals; D001667:Binding, Competitive; D002645:Chickens; D003600:Cytosol / Q000378:metabolism; D013196:Dihydrotestosterone / Q000031:analogs & derivatives / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D010057:Oviducts / Q000378:metabolism*; D011944:Receptors, Androgen / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D013379:Substrate Specificity
chemical_list: D000345:Affinity Labels; D011944:Receptors, Androgen; D013196:Dihydrotestosterone; C023562:dihydrotestosterone 17-bromoacetate