Precise in situ localization of NCAM, ETS1, and D11S29 on human meiotic chromosomes.
In order to sublocalize NCAM, ETS1, and the anonymous DNA fragment D11S29 within 11q23, in situ hybridization was performed on pachytene bivalents. Analysis of the grain distribution within the band 11q23 indicated that the chromosomal sublocalization of both NCAM and D11S29 was in 11q23.1, whereas ETS1 was found to be localized in 11q23.3. These results clearly demonstrate the usefulness of in situ hybridization applied to pachytene bivalents to obtain accurate gene sublocalization.
Bello
M J
MJ
Laboratoire d'Embryologie et de Cytogénétique, Faculté de Médecine, Secteur Nord, France.
Salagnon
N
N
Rey
J A
JA
Guichaoua
M R
MR
Bergé-Lefranc
J L
JL
Jordan
B R
BR
Luciani
J M
JM
D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D004247:DNA / Q000235:genetics*; D015342:DNA Probes; D006023:Glycoproteins / Q000235:genetics; D006801:Humans; D008297:Male; D008540:Meiosis; D008565:Membrane Proteins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D011519:Proto-Oncogenes / Q000235:genetics*; D013737:Testis / Q000648:ultrastructure
D015342:DNA Probes; D006023:Glycoproteins; D008565:Membrane Proteins; D004247:DNA