Cytosine arabinoside plus hemin treatment of a human erythroid cell line, KMOE, strongly induces embryonic, fetal, and adult beta-like globin genes.
A variety of regimens were utilized on KMOE cells to maximally raise globin mRNA levels for the purpose of improving the usefullness of this line for globin gene studies. Steady-state mRNA levels of embryonic (epsilon), fetal (gamma) and adult (beta) globin genes were assayed by the S1-nuclease protection method before and after exposure to inducing compounds. Exposure of KMOE cells to cytosine arabinoside and hemin leads to over 20-fold increases in beta- and gamma-globin mRNA steady-state levels, and an over 60-fold increase in epsilon-globin mRNA level. Exposure to cytosine arabinoside alone induced beta- and epsilon-globin but not gamma-globin gene expression. The alpha-like globin genes (zeta and alpha) were also monitored but found to be poorly expressed and not significantly inducible. The presence of epsilon-globin mRNA and the lack of alpha-globin mRNA distinguishes this line, KMOE-EL, from the KMOE sublines previously described.
Stoeckert
C J
CJ
Dept. of Anatomy, University of Pennsylvania School of Medicine, Philadelphia 19104.
Nicolaides
N C
NC
Haines
K M
KM
D000622:Aminolevulinic Acid / Q000494:pharmacology; D003561:Cytarabine / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004357:Drug Synergism; D015672:Erythroid Precursor Cells / Q000187:drug effects* / Q000378:metabolism; D004921:Erythropoietin / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects*; D005914:Globins / Q000096:biosynthesis / Q000235:genetics*; D006418:Heme / Q000031:analogs & derivatives*; D006427:Hemin / Q000494:pharmacology*; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis; D013268:Stimulation, Chemical; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
D012333:RNA, Messenger; D003561:Cytarabine; D004921:Erythropoietin; D006418:Heme; D006427:Hemin; D000622:Aminolevulinic Acid; D005914:Globins; D004121:Dimethyl Sulfoxide