A high yield method for the isolation of sheep's liver cathepsin L.
A method, giving twice the yield of the previous method, for the isolation of sheep's liver cathepsin L is described. The method uses three phase partitioning (TPP) in t-butanol/water/ammonium sulphate mixtures, followed by two chromatographic steps, at different pH values, in a single column of S-Sepharose.
Pike
R
R
Department of Biochemistry, University of Natal, Pietermaritzburg, South Africa.
Dennison
C
C
D000818:Animals; D056668:Cathepsin L; D002403:Cathepsins / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000378:metabolism; D005591:Chemical Fractionation; D002852:Chromatography, Ion Exchange; D003546:Cysteine Endopeptidases; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000201:enzymology*; D012756:Sheep
D002403:Cathepsins; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; D056668:Cathepsin L