Nucleotide sequence of SN-07 chromophore binding site.
DNase I footprinting was used to investigate binding sites for SN-07 chromophore on DNA fragments prepared from pBR322. Six sites were protected on about 150 base pair DNA fragments by SN-07 chromophore, but not by related anthracycline antibiotics from DNase I digestion. All the protected sites contained the dinucleotide sequence 5'-GC-3', but no other regularities could be discerned. A drug-induced conformational change of DNA was suggested by enhancement of DNase I sensitivity between the protected sites. These results support covalent interaction of the carbinolamine function of SN-07 chromophore to 2-amino group of guanine residues.
Kimura
K
K
Research Institute of Life Science, Snow Brand Milk Products Co., Ltd., Tochigi, Japan.
Morinaga
T
T
Miyata
N
N
Kawanishi
G
G
D018943:Anthracyclines; D000903:Antibiotics, Antineoplastic / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000378:metabolism*; D003850:Deoxyribonuclease I; D004591:Electrophoresis, Polyacrylamide Gel
D018943:Anthracyclines; D000903:Antibiotics, Antineoplastic; C047795:SN-07; D004247:DNA; D003850:Deoxyribonuclease I