Purification and N-terminal sequence of two tartrate-resistant acid phosphatases type-5 from the hairy cell leukemia spleen.
Tartrate-resistant acid phosphatases types 5a and 5b were purified from human hairy cell leukemia spleen by sequential chromatography on Phenyl-Sepharose, CM-Sepharose, concanavalin A-Sepharose, FPLC Superose-12 and FPLC Mono-S. The purification over the original tissue extract was 1150- and 3300-fold, with a yield of 2.1% and 2.5%, respectively. Gel filtration indicated an Mr of about 30000 for both forms. There was a N-terminal sequence identity between the two enzymes. However, they appeared to be different as assessed by cation exchange chromatography and amino acid composition.
Stĕpán
J J
JJ
Department of Medicine, Loma Linda University, CA.
Lau
K H
KH
Mohan
S
S
Kraenzlin
M
M
Baylink
D J
DJ
D000135:Acid Phosphatase / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D004351:Drug Resistance; D006801:Humans; D007943:Leukemia, Hairy Cell / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D008970:Molecular Weight; D013154:Spleen / Q000201:enzymology*; D013644:Tartrates / Q000494:pharmacology*
D000596:Amino Acids; D013644:Tartrates; D000135:Acid Phosphatase; C029768:tartaric acid