A re-investigation of the structure of the capsular polysaccharide of Klebsiella K10.
The capsular polysaccharide of Klebsiella K10 was investigated by methylation analysis and 1H-n.m.r. spectroscopy, and the deacetylated bacteriophage-degraded polysaccharide by 1D- and 2D-n.m.r. spectroscopy. The repeating unit was shown to be a branched hexasaccharide (see text). OAc substituents were located on the terminal and 2-linked galactopyranosyl residues by Prehm methylation of a low-molecular-weight fraction obtained by bacteriophage degradation.
Dutton
G G
GG
Department of Chemistry, University of British Columbia, Vancouver.
Ng
S K
SK
Parolis
L A
LA
Parolis
H
H
Chakraborty
A K
AK
D001435:Bacteriophages; D002240:Carbohydrate Sequence; D007709:Klebsiella / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification
D011135:Polysaccharides, Bacterial