Effect of Staphylococcus aureus serine proteinase on human lymphocyte impairment.
Assessment of lymphocyte surface membrane integrity by means of trypan exclusion test and longer term survival studies based on 3HTdR uptake and Con A stimulation test were used to estimate effect of Staphylococcus aureus serine proteinase on human lymphocytes in vitro. At concentration of proteinase as high as 10 micrograms/ml, the percentage of trypan-stained lymphocytes increase with enzyme level. In contrast, decrease of 3HTdR level and Con A stimulation is already apparent at concentrations of proteinase ten times lower. Autologous serum exerts strong protective effect. Observed impairment is discussed as significant for local response in inflammation.
Turyna
B
B
Department of Animal Biochemistry, Jan Zurzycki Institute of Molecular Biology, Jagellonian University, Kraków, Poland.
Ochałek
T
T
D006801:Humans; D066298:In Vitro Techniques; D007963:Leukocytes, Mononuclear / Q000187:drug effects*; D012697:Serine Endopeptidases / Q000494:pharmacology*
D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase