[Hairy cell leukemia: study of genomic rearrangements in splenic infiltration].
Genomic DNA extracted from heavily infiltrated spleens of 5 patients with hairy cell leukaemia was hybridized with genomic probes for immunoglobulin genes. The rearrangement pattern, correlating well with the immunohistochemical findings, was that of a clonal B-cell proliferation in all cases. Analysis of the lambda light chain genome with two different probes enabled more accurate localization of the clonal rearrangements within this genome. A small additional T-cell population identified in 3 cases was immunogenotypic of polyclonal (reactive) nature.
Knecht
H
H
Département de médecine interne, CHUV, Lausanne.
Odermatt
B F
BF
Hayoz
D
D
Bachmann
F
F
D015342:DNA Probes; D004273:DNA, Neoplasm / Q000235:genetics; D015328:Gene Rearrangement, B-Lymphocyte, Light Chain / Q000235:genetics*; D015329:Gene Rearrangement, T-Lymphocyte / Q000235:genetics; D006801:Humans; D007943:Leukemia, Hairy Cell / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D013154:Spleen / Q000166:cytology
D015342:DNA Probes; D004273:DNA, Neoplasm
Leucémie à tricholeucocytes (hairy cell leukaemia): étude des réarrangements génomiques de l'infiltration splénique.