Genomic organization of the region encoding guinea pig lipoprotein lipase; evidence for exon fusion and unconventional splicing.
The coding sequence of guinea pig lipoprotein lipase (LPL) is organized into nine exons and spans a region of approximately 14 kb of the guinea pig genome. A non-conforming 5'-splice site is located on the first intron, which exhibits a 12-nucleotide perfect match with the 5'-end of the second exon. A previously described tryptic cleavage site is located on exon V, close to the 3' end of this exon. A similarity to vitellogenin resides on exons IV and V, and a putative active site is found on exon IV. A novel similarity to a fatty-acid-binding protein is noted on exon VI, adjacent to the postulated heparin-binding region. We suggest that free fatty acids (FFA) and heparin to some extent share the same site of interaction on the LPL molecule; and that a high local concentration of FFA can displace LPL from its site of action--the vascular endothelium--by competing for binding to heparan sulfate.
Enerbäck
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Department of Medical Biochemistry, University of Göteborg, Sweden.
Bjursell
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001667:Binding, Competitive; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D004274:DNA, Recombinant; D005091:Exons; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005796:Genes; D006168:Guinea Pigs; D006493:Heparin / Q000378:metabolism; D006801:Humans; D007438:Introns; D008071:Lipoprotein Lipase / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D012326:RNA Splicing; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D025521:Tumor Suppressor Proteins; D000516:alpha-Amylases / Q000235:genetics
D002352:Carrier Proteins; D004274:DNA, Recombinant; C107608:FABP7 protein, human; C082667:Fabp5 protein, mouse; C086936:Fabp7 protein, mouse; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D025521:Tumor Suppressor Proteins; D006493:Heparin; D008071:Lipoprotein Lipase; D000516:alpha-Amylases