title: Fate of allogeneic embryonal chick chondrocytes implanted orthotopically, as determined by the host's age.
abstract: Chondrocytes derived from chick embryos can be successfully implanted in defects of adult chick articular cartilage surfaces. Such implants thrive in their implantation site and create a new articular surface. The chondrocytes mature and hypertrophy in the orthotopic site without invoking an immune response. Eventually a steady state is reached in which mature chondrocytes resurface the defect while in the deeper areas spongy bone replaces the hypertrophic chondrocytes. Time schedules of these repair events have been studied in hosts of different ages. We compared 4-month-old chicks with 3-year-old chickens. The embryonal chondrocytes implanted in the latter group underwent an accelerated aging process. The defects were completely filled-up after 1 month as compared with 2-3 months in the younger age group. Endochondral ossification in the older group was evident as early as 2 months post implantation and was completed after 6 months. This contrasts with the situation in the younger group where the chondrocytes only began to hypertrophy after 6 months. At this stage endochondral ossification was hardly seen at all. A unique response to the cartilaginous implants is seen in the old group only in the vicinity of the reparative tissue, accumulation of hematopoietic centers. This study seem to indicate that the host's environment affects the "biological clock", i.e. rate and degree of aging of the implanted cells, as well as their matrices.
authors.affiliation: Department of Orthopedic Surgery A, Assaf Harofeh Medical Center, Zerifin, Israel.
authors.forename: D
authors.initials: D
authors.lastname: Robinson
authors.forename: N
authors.initials: N
authors.lastname: Halperin
authors.forename: Z
authors.initials: Z
authors.lastname: Nevo
mesh_terms: D000367:Age Factors; D000818:Animals; D002358:Cartilage, Articular / Q000196:embryology / Q000637:transplantation*; D002642:Chick Embryo; D051381:Rats; D013997:Time Factors; D014184:Transplantation, Homologous