Annual changes in the number, testosterone content and ultrastructure of glandular tissue cells of the testis in the marbled newt Triturus marmoratus.
The testes of 8 specimens of Triturus marmoratus were collected during each month of 1987 and processed for electron microscopy and light microscopy demonstration of testosterone (T) following the ABC (avidin-biotin peroxidase complex) method. According to their staining affinity for anti-T antibodies, the glandular tissue cells were classified as T-, T+, T++, and T and the annual changes in the numbers of these cell populations, as well as in the volume occupied by the glandular tissue, were calculated. The volume occupied by the glandular tissue increases from September to December; it begins to decrease in April and disappears from June to August. The glandular tissue is formed from the interstitial cells that surround the lobules containing differentiating germ cells. During the spermatogenic process, the interstitial cells do not show staining affinity for anti-T antibodies. In August-September, the interstitial cells around the lobules that have completed spermatogenesis become positively stained (T+) and form the glandular tissue when the spermatozoa leave the testis. The numbers of intensely stained cells in the glandular tissue (T++ and T ) increase from September to November; begin to decrease in December; disappear in January-February; increase again in March and decrease again in April until they disappear in June-September. The interstitial cells, before their transformation in glandular tissue, are ultrastructurally similar to fibroblasts. After their transformation these cells increase in size and develop abundant smooth endoplasmic reticulum, mitochondria with tubular cristae and lipid droplets. This morphological pattern is maintained in the glandular tissue from September to April in spite of the changes in staining affinity during this period.
Fraile
B
B
Department of Cell Biology and Genetics, University of Alcalá de Henares, Madrid, Spain.
Paniagua
R
R
Rodríguez
M C
MC
Sáez
F J
FJ
Jimenez
A
A
D000818:Animals; D002452:Cell Count; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D008928:Mitochondria / Q000648:ultrastructure; D012621:Seasons; D013091:Spermatogenesis; D013094:Spermatozoa / Q000648:ultrastructure; D013737:Testis / Q000502:physiology / Q000648:ultrastructure*; D013739:Testosterone / Q000502:physiology*; D014319:Triturus / Q000033:anatomy & histology* / Q000502:physiology
D013739:Testosterone