Increased forskolin stimulation of lymphocyte-adenylate cyclase in normotensive subjects predisposed to essential hypertension.
Since a significant heritability has been shown for forskolin stimulation of lymphocyte adenylate cyclase activity in twins, we evaluated lymphocyte forskolin-stimulated adenylate cyclase activity with respect to a familial predisposition towards essential hypertension. Lymphocyte adenylate cyclase activity was measured in broken cell preparations of 32 male normotensive volunteers with (n = 15) and without (n = 17) a positive family history of hypertension. The maximal forskolin stimulation of adenylate cyclase activity was significantly higher in the positive compared with the negative group (maximal stimulation of activity 53.5 +/- 3.4 versus 41.2 +/- 1.9 pmol cyclic AMP (cAMP)/mg protein per min; P less than 0.01). Dose-response curves showed a significantly greater stimulation of adenylate cyclase activity in the positive group at forskolin concentrations of 10(-7) to 2 x 10(-4) mol/l. The median effective dose (ED50) and adenylate cyclase activity in the absence of forskolin were similar in both groups. We conclude that lymphocyte forskolin-stimulated adenylate cyclase activity may depend in part on hereditary factors associated with a familial predisposition to essential hypertension.
Schultz
K D
KD
Department of Internal Medicine, Klinikum Steglitz, Freie Universität Berlin, Federal Republic of Germany.
Fritschka
E
E
Pauliks
L B
LB
Philipp
T
T
Distler
A
A
D000262:Adenylyl Cyclases / Q000097:blood*; D005576:Colforsin; D004198:Disease Susceptibility / Q000175:diagnosis / Q000201:enzymology; D004305:Dose-Response Relationship, Drug; D020022:Genetic Predisposition to Disease; D006801:Humans; D006973:Hypertension / Q000175:diagnosis / Q000201:enzymology* / Q000235:genetics; D008214:Lymphocytes / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D013268:Stimulation, Chemical
D005576:Colforsin; D000262:Adenylyl Cyclases