[The capping of receptors for the epidermal growth factor and the participation of the cytoskeleton in this process in A-431 cells].
Epidermal growth factor (EGF) receptor capping results from the interaction between the receptors and polyvalent ligands in A-431 cells examined in suspension at 22 degrees C. Colocalization of actin and spectrin with the ligand-receptor complexes during the redistribution was shown using double immunofluorescence. The obtained data show that the cortical microfilaments are involved in capping. EGF receptors become associated with the Triton-insoluble cytoskeleton as a consequence of ligand binding. EGF-receptor capping is not sensitive to the action of cytochalasin B. Capping in A-431 cells is discussed as a new model for studying the redistribution of the ligand-receptor complex.
Khrebtukova
I A
IA
Nikol'skaia
A N
AN
Sorkin
A D
AD
Sorokin
A B
AB
Pinaev
G P
GP
D000199:Actins / Q000502:physiology; D000906:Antibodies / Q000494:pharmacology; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002294:Carcinoma, Squamous Cell / Q000503:physiopathology*; D002460:Cell Line; D003571:Cytochalasin B / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects / Q000502:physiology*; D004815:Epidermal Growth Factor / Q000276:immunology / Q000494:pharmacology; D066246:ErbB Receptors / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D008024:Ligands; D011940:Receptor Aggregation / Q000187:drug effects / Q000502:physiology*; D013049:Spectrin / Q000502:physiology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000502:physiology
D000199:Actins; D000906:Antibodies; D000911:Antibodies, Monoclonal; D007106:Immune Sera; D008024:Ligands; D013049:Spectrin; D003571:Cytochalasin B; D004815:Epidermal Growth Factor; D066246:ErbB Receptors
Képping retseptorov k épidermal'nomu faktoru rosta i uchastie tsitoskeleta v étom protsesse u kletok A-431.