A rapid procedure for the purification of IgA1 and IgA2 subclasses from normal human serum using protein G and jackfruit lectin (jacalin) affinity chromatography.
Immunoglobulin A (IgA) from pooled normal human sera was purified using antibody and protein G affinity chromatography and gel filtration high-pressure liquid chromatography (HPLC). This high purity product was separated into IgA1 and IgA2 subclasses utilizing the agarose-bound lectin 'jacalin'. Evaluation of product homogeneity by immunological testing confirmed greater than 95% purity. The total IgA1 and IgA2 recovered from sera was approximately 26% of the initial antibody present.
Haun
M
M
Protein Chemistry Section, Canadian Red Cross Society, Ottawa, Ontario.
Incledon
B
B
Alles
P
P
Wasi
S
S
D002846:Chromatography, Affinity / Q000379:methods*; D002847:Chromatography, Agarose; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D006801:Humans; D007070:Immunoglobulin A / Q000145:classification / Q000302:isolation & purification*; D037102:Lectins; D009419:Nerve Tissue Proteins; D037121:Plant Lectins
C029152:G-substrate; D007070:Immunoglobulin A; D037102:Lectins; D009419:Nerve Tissue Proteins; D037121:Plant Lectins; C043696:jacalin