title: DNA sequence and pattern of expression of the sea urchin (Paracentrotus lividus) alpha-tubulin genes.
abstract: To study the molecular aspects of the regulation of transcription of a multigene family, we have isolated and sequenced cDNA and genomic clones coding for the alpha-tubulin of the sea urchin Paracentrotus lividus. Two cDNA clones, P alpha 10 and P alpha 4, contain respectively the coding information for 391 C-terminal and for 338 N-terminal amino acids of the 452 residues that constitute the complete protein. They show silent nucleotide substitutions only, suggesting that P alpha 10 and P alpha 4 represent the cloned copies of two allelic gene transcripts, which encode for two alpha-tubulin isoforms with identical amino acid sequence in the region of the overlap. The comparison of the predicted amino acid sequence of the composite P alpha 4-10 and of the mouse M alpha-6 (Villasante et al., Mol Cell Biol 1986; 6:2409-2419) reveals a conservation of 97% between the two polypeptides. By RNA blotting hybridization six major alpha-tubulin transcripts were identified. Two, of 3.5 kb and 2.0 kb, are expressed in the unfertilized eggs and during early cleavage. The other two maternal mRNAs, of 2.4 kb and 1.8 kb, are expressed in both early and late cleavage embryos, but in the intestine the 1.8 kb RNA, which specifically reacted with the 3' specific probe of the P alpha 10 cDNA, is the only transcript detected. Finally, the 1.5 kb and 1.9 kb mRNAs represent the transcription of stage- and tissue-specific genes, respectively. In fact, the former becomes detectable at blastula stage and accumulates during late development, whereas the latter is found in the testis only. The sequence data of the 3' terminus of the alpha-3 genomic clone suggests that it encodes for a divergent alpha-tubulin, and it most probably corresponds to the testis-specific gene.
authors.affiliation: Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Palermo, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Gianguzza
authors.forename: M G
authors.initials: MG
authors.lastname: Di Bernardo
authors.forename: M
authors.initials: M
authors.lastname: Sollazzo
authors.forename: F
authors.initials: F
authors.lastname: Palla
authors.forename: M
authors.initials: M
authors.lastname: Ciaccio
authors.forename: E
authors.initials: E
authors.lastname: Carra
authors.forename: G
authors.initials: G
authors.lastname: Spinelli
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D004247:DNA / Q000235:genetics*; D015342:DNA Probes; D005786:Gene Expression Regulation; D008322:Mammals / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D012617:Sea Urchins / Q000196:embryology / Q000235:genetics*; D014404:Tubulin / Q000235:genetics*
chemical_list: D015342:DNA Probes; D012333:RNA, Messenger; D014404:Tubulin; D004247:DNA