title: DNA-content, inflammatory tissue response and tumour size in human lung carcinoma.
abstract: Tumour imprints of 75 resection specimens with human lung carcinoma (lobe and lungs) were fixed with alcohol and Feulgen-stained. Resection specimens were cut into serial sections 6 mm thick and tumour mass, tumour involvement into intrapulmonary and extrapulmonary lymph nodes, detailed pTN-stage were determined. DNA-content, DNA-index, percentage of diploid/hyperploid tumour cells, and morphometric nuclear features were measured using an automated image analyzing system (VISIAC). Only 10% of the measured carcinomas were diploid. The DNA-index showed the broadest variance in large cell anaplastic carcinoma (1.2-3.3). Carcinomas growing predominantly within the alveolar space, i.e. without destroying the interstitial tissue showed a lower DNA-content above 3c and above 5c compared to carcinomas destroying the interstitial tissue. Carcinomas with severe stroma reaction were found to have 33%-48% of DNA above the 3c value whereas carcinomas without stromal reaction had a percentage above 3c ranging 46%-64% (confidence limits, p less than 0.05). DNA-index increases with increasing tumour volume and decreases for large tumours (greater than 100 ccm). Hyperploid and polyploid tumours were found more frequently in case of pT2 and pT3-stages compared to pT1-stages. No relation of DNA-content or ploidy was found to lymph node involvement and inflammatory response of host tissue.
authors.affiliation: Department of Pathology, Thoraxklinik Heidelberg, Rohrbach, FRG.
authors.forename: K
authors.initials: K
authors.lastname: Kayser
authors.forename: A
authors.initials: A
authors.lastname: Schmidt
authors.forename: H
authors.initials: H
authors.lastname: Stute
authors.forename: S
authors.initials: S
authors.lastname: Bach
mesh_terms: D002283:Carcinoma, Bronchogenic / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007249:Inflammation / Q000209:etiology; D008175:Lung Neoplasms / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D008214:Lymphocytes / Q000032:analysis; D008297:Male; D011003:Ploidies
chemical_list: D004273:DNA, Neoplasm