title: A potexvirus isolated from Silene pratensis.
abstract: A potexvirus, silene virus X (SVX), was isolated from the weed Silene pratensis. Infectious preparations contained nucleoprotein rods predominantly 546 nm in length, consisting of a 6.9-kb polyadenylated single-stranded RNA and a 29-kd capsid protein. Double-stranded RNAs of 17.1, 7.0, 5.7, and 4.6 kbp were isolated from SVX-infected plants. In vitro translation products of SVX single-stranded or denatured double-stranded RNAs reacted with SVX antiserum. Hybridization studies with SVX cDNAs showed no nucleotide homology with seven other potexviruses. The virus is more closely related serologically to narcissus mosaic virus than to nine other potexviruses.
authors.affiliation: Agriculture Canada Research Station, Vancouver, B.C., Canada.
authors.forename: D A
authors.initials: DA
authors.lastname: Wakarchuk
authors.forename: R I
authors.initials: RI
authors.lastname: Hamilton
mesh_terms: D002213:Capsid / Q000032:analysis; D002499:Centrifugation, Density Gradient; D002712:Chlorides; D008854:Microscopy, Electron; D010942:Plant Viruses / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D014176:Protein Biosynthesis; D012330:RNA, Double-Stranded / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D014764:Viral Proteins / Q000032:analysis
chemical_list: D002712:Chlorides; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D014764:Viral Proteins