Chemical composition of a lipopolysaccharide from Legionella pneumophila.
Lipopolysaccharide isolated from Legionella pneumophila (Phil. 1) was examined for chemical composition. The polysaccharide split off by mild acid hydrolysis contained rhamnose, mannose, glucose, quinovosamine, glucosamine and 2-keto-3-deoxyoctonate, in molar proportions 1.6:1.8:1.0:1.5:4.1:2.7. Heptoses were absent and glucose was probably mainly phosphorylated. The carbohydrate backbone of the lipid A part consisted of glucosamine, quinovosamine and glycerol, in the molar ratios 3.9:1.0:3.4, with glycerol as a phosphorylated moiety. A complex fatty acid substitution pattern comprising eight O-ester-linked, exclusively nonhydroxylated acids, and nineteen amide-linked, exclusively 3-hydroxylated acids was revealed. Both straight- and branched (iso and anteiso) carbon chains occurred. The major hydroxy fatty acid was 3-hydroxy-12-methyltridecanoic acid and six others were of a chain-length above 20 carbon atoms, with 3-hydroxy-20-methyldocosanoic acid as the longest. Two dihydroxy fatty acids, 2,3-dihydroxy-12-methyltridecanoic and 2,3-dihydroxytetradecanoic acids, were also detected. These results suggest that L. pneumophila contains a rather complex and unusual lipopolysaccharide structure of considerable biological and chemotaxonomic interest.
Sonesson
A
A
Department of Technical Analytical Chemistry, Chemical Center, Lund University, Sweden.
Jantzen
E
E
Bryn
K
K
Larsson
L
L
Eng
J
J
D002241:Carbohydrates / Q000032:analysis; D002849:Chromatography, Gas; D005227:Fatty Acids / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D006868:Hydrolysis; D007875:Legionella / Q000032:analysis*; D008050:Lipid A / Q000032:analysis; D008070:Lipopolysaccharides / Q000032:analysis*; D011135:Polysaccharides, Bacterial / Q000032:analysis
D002241:Carbohydrates; D005227:Fatty Acids; D008050:Lipid A; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial