Effect of a traditional Chinese herbal medicine (san zhuang wan) on the hypothalamus-pituitary-testis axis of the immature rat.
San Zhuang Wan (SZW) is a traditional Chinese herbal medicine used to treat male impotence. The mechanism of its therapeutic effect is not known nor have any of its potential physiologic actions been investigated. We examined the effect of SZW administered by gavage, on the reproductive system of the prepubertal male rat. After 10 days treatment of 21-day-old rats the concentration of testosterone in serum and testicular weight had decreased whereas the serum concentration of luteinizing hormone (LH) had significantly increased. When administered to gonadectomized rats SZW partially restored the weight of the accessory sex organs and concentration of LH toward normal, but serum androgen concentration was not changed. In vitro assessment of fragments of testicular tissue or purified Leydig cells for responsiveness to maximal doses of LH or cyclic AMP showed that SZW significantly reduced testosterone secretion in response to either stimulant. The data demonstrate that SZW has potent effects on the reproductive axis of the pubertal male rat. At least one locus for such action is on the Leydig cell at a point distal to the formation of cyclic AMP. Further study is necessary to determine whether these effects could be involved with or independent of the mechanism of the therapeutic action of SZW.
Zhang
T X
TX
Department of Physiology, Tianjin Institute of Medical and Pharmaceutical Science, China.
Pomerantz
D K
DK
D000818:Animals; D002369:Castration; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007030:Hypothalamo-Hypophyseal System / Q000187:drug effects*; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D009929:Organ Size; D051381:Rats; D011919:Rats, Inbred Strains; D013737:Testis / Q000033:anatomy & histology / Q000187:drug effects*; D013739:Testosterone / Q000097:blood
D004365:Drugs, Chinese Herbal; D013739:Testosterone; D007986:Luteinizing Hormone