title: Rearrangement of the immunoglobulin kappa light chain genes in a b4 rabbit and a Basilea rabbit.
abstract: The immunoglobulin chi light chain gene family of the rabbit is characterized by the presence of two constant region exons, C chi 1 and C chi 2 encoded at the chi 1 and chi 2 loci, and linked to their own cluster of joining pieces (J chi). The gene segments at the two loci are very unequally expressed. Thus, in domestic rabbits, the immunoglobulin light chains are essentially of the chi 1 type, even though the gene segments at the chi 2 locus are structurally functional. We have investigated the origin of the weak expression of the genes at the chi 2 locus by analysing the pattern of rearrangement of the chi 1 and chi 2 J chi segments in rabbit B-cell populations. Southern blot analysis of B cells isolated from a rabbit expressing chi 1 light chains suggests that the genes at the chi 2 locus underwent very few, if any, rearrangements. However, using more sensitive approaches, it was possible to detect transcripts originating from the rearranged chi 2 locus. In contrast, in B cells isolated from a Basilea rabbit, which cannot express chi 1 chains, Southern blots revealed the rearrangement of the chi 2 genes, whereas the chi 1 rearranged fragments were barely detectable. These results could be explained either by preferential rearrangement of genes at the chi 1 locus or by clonal amplification of only cells producing chi 1. Furthermore, results of Southern blot analysis provide evidence that V-J recombination may be accompanied by an inversion of the intervening DNA region.
authors.affiliation: Unité de Génétique et de Biochimie du Développement, LACNRS, Département d'Immunologie, Institut Pasteur, Paris.
authors.forename: M A
authors.initials: MA
authors.lastname: Akimenko
authors.forename: B
authors.initials: B
authors.lastname: Mariamé
authors.forename: F
authors.initials: F
authors.lastname: Rougeon
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D015328:Gene Rearrangement, B-Lymphocyte, Light Chain; D006720:Homozygote; D008957:Models, Genetic; D008969:Molecular Sequence Data; D011817:Rabbits / Q000235:genetics* / Q000276:immunology
chemical_list: D004247:DNA