[Changes in antigenic properties of the p35 protein of vaccinia virus in various protein fractions of the virion with the use of monospecific antisera].
Three monospecific antisera to the major 35 kD (p35) surface protein of vaccinia and ectromelia viruses have been obtained. Two of them are obtained to p35 protein isolated by electrophoresis in the presence of sodium dodecylsulfate from the protein fractions of vaccinia virus, soluble in NP40 and NP40 with dithiothreitol (NP40 and DTT-fractions). The third serum is obtained to NP40-fraction of ectromelia virus, containing practically only p35 protein. The obtained antisera were compared in the reactions with the different fractions of viral proteins in two versions of solid phase radioimmunoassay. The effect of such reagents as sodium dodecylsulfate, NP40, 2-mercaptoethanol, ethanol on the antigenic properties of p35 protein from vaccinia virus is discussed.
Muravlev
A I
AI
Poryvaeva
V A
VA
Reshetnikov
S S
SS
D000956:Antigens, Viral / Q000276:immunology*; D004481:Ectromelia virus / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D007106:Immune Sera; D011863:Radioimmunoassay; D014616:Vaccinia virus / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology*; D014771:Virion / Q000276:immunology*
D000956:Antigens, Viral; D007106:Immune Sera; D014764:Viral Proteins
Izmenenie antigennykh svoĭstv belka P35 virusa ospovaktsiny v sostave razlichnykh belkovykh fraktsiĭ viriona s pomoshch'iu monospetsificheskikh antisyvorotok.