Effect of ionic charge on detergent-induced hemolysis.
7.0 microM Sodium dodecyl sulfate induces lysis on A Rh(+) red blood cells 1.6 times faster than the cationic analog dodecyl thiouronium chloride at 37 degrees C and pH = 7.30. Hemolysis isotherms show identical values for the Hill cooperativity parameters of the organic surfactants, but different dissociation parameters. The anionic detergent destabilizes red cell membranes more efficiently than the cationic analog, probably due to the predominance of negative charges on the erythrocyte membranes at physiological pH.
Pazos-Sanou
L
L
Department of Physiology, University of Costa Rica.
Mata-Segreda
J F
JF
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects; D003902:Detergents / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects*; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D009861:Onium Compounds / Q000494:pharmacology*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*; D013452:Sulfonium Compounds / Q000494:pharmacology*; D013501:Surface-Active Agents / Q000494:pharmacology*
D003902:Detergents; D009861:Onium Compounds; D013452:Sulfonium Compounds; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; C062864:S-dodecyl thiouronium