[Enterobacterial antigen in human peripheral blood lymphocytes].
The following study has as prior history the research reports which have shown the existence of an antigenic tissue deposit in gram-negative enterobacteria. The antigens of the enterobacteria have also been found in the lymphocytic membranes and cytoplasm. Since intestinal lymphoid tissue cells can recirculate by means of the thoracic duct to the peripheral venous system, it was proposed that the circulating lymphocytes in healthy people could also contain small amounts of a common enterobacterial antigen. The study was carried out in 15 human venous blood samples, of which the lymphocytic population was separated to later be used in the preparation of 15 alcohol soluble extracts. This material was used for inhibiting the immuno-hemolysis assay in three occasions in order to show the presence of antigens shared by different enterobacterias, using as reference a fraction separated from the LPS of Escherichia coli 08. The results showed that the human lymphocytes also had antigenic determinants common to gram-negative bacteria.
Faure-Fontenla
M A
MA
García-Tamayo
F
F
D000942:Antigens, Bacterial / Q000032:analysis*; D015941:Complement Hemolytic Activity Assay; D006801:Humans; D008214:Lymphocytes / Q000032:analysis*
D000942:Antigens, Bacterial; C017286:enterobacterial common antigen
Un antígeno de enterobacterias en los linfocitos de sangre periférica humana.