Determination of cocaine and selected metabolites in canine and human serum by reversed-phase high-performance liquid chromatography on coupled cyanopropyl and silica columns.
A high-performance liquid chromatographic procedure for the determination of cocaine and selected metabolites (benzoylecgonine, norcocaine and benzoylnorecgonine) from human and canine serum has been developed. The analytes are extracted from 0.5-ml serum samples using strong cation-exchange and octadecylsilane solid-phase extraction columns. Chromatographic separation was accomplished on coupled cyanopropyl and silica columns using acetonitrile-6.25 mM phosphate buffer, pH 2.9 (80:20 v/v) as eluent and a flow-rate of 1.1 ml/min. Low-wavelength UV detection (228 nm, cadmium lamp) allowed detection of 1 ng/ml for each analyte. The assay was linear from 0 to 200 ng/ml (r2 = 0.997-0.999, n = 15 for each analyte) and showed good precision (relative standard deviation = 0.66-13.8%, n = 6) and accuracy (98-115% relative recovery).
Lampert
B M
BM
Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Georgia, Athens 30602.
Stewart
J T
JT
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003042:Cocaine / Q000031:analogs & derivatives / Q000097:blood* / Q000493:pharmacokinetics; D004285:Dogs; D006801:Humans; D013056:Spectrophotometry, Ultraviolet
C008671:norcocaine; C005618:benzoylecgonine; D003042:Cocaine; C012209:benzoylnorecgonine