title: A method for the simultaneous analysis of unconjugated and glycine-conjugated bile acids by capillary gas-liquid chromatography.
abstract: A method for the simultaneous analysis of unconjugated and glycine-conjugated bile acids by means of capillary gas-liquid chromatography without need for prior deconjugation is described. The method involves: i) the use of an aluminum-clad fused-silica capillary column coated with a very thin film (0.1 micron) of a highly thermostable bonded and crosslinked methyl polysiloxane, and ii) the analysis of the bile acids as their methyl ester-dimethylethylsilyl ether derivatives. This method, used to separate the major free and glycine-conjugated bile acids from human gall bladder bile, should be applicable for the analysis of other biological fluids.
authors.affiliation: College of Engineering, Nihon University, Fukushima-ken, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Iida
authors.forename: T
authors.initials: T
authors.lastname: Itoh
authors.forename: K
authors.initials: K
authors.lastname: Hagiwara
authors.forename: F C
authors.initials: FC
authors.lastname: Chang
authors.forename: J
authors.initials: J
authors.lastname: Goto
authors.forename: T
authors.initials: T
authors.lastname: Nambara
mesh_terms: D001647:Bile Acids and Salts / Q000032:analysis* / Q000378:metabolism; D002849:Chromatography, Gas / Q000379:methods*; D005704:Gallbladder / Q000032:analysis; D005998:Glycine / Q000378:metabolism*; D006801:Humans
chemical_list: D001647:Bile Acids and Salts; D005998:Glycine