Transmembrane migration technique: reexamination of its usefulness in sperm motility assessments.
A study was carried out using time-exposure photomicrography to investigate the potential usefulness of the transmembrane migration technique in the assessment of drug effects on human sperm motility. Significant but weak correlations were evident between the transmembrane migration ratio (TMMR%) and both % motility (r = 0.43; p less than 0.05) and the amplitude of lateral sperm head displacement (r = 0.40; p less than 0.05). However, no significant correlations were evident between TMMR% and the concentration of motile spermatozoa, mean path velocity, or frequency of sperm head rotation. Exposure to 2.5 mM 2-deoxyadenosine caused significant increases in % motility, mean path velocity, and frequency of sperm head rotation, with no concomitant rise in TMMR%, while 5.0 mM caffeine caused significant elevations in both TMMR% and in % motile cells. It is concluded that the transmembrane migration technique is a relatively insensitive method for detecting subtle but important changes in the movement characteristics of human spermatozoa.
Ratnasooriya
W D
WD
MRC Unit of Reproductive Biology, Edinburgh, UK.
Aitken
R J
RJ
D002110:Caffeine / Q000494:pharmacology; D003839:Deoxyadenosines / Q000494:pharmacology; D005069:Evaluation Studies as Topic; D006801:Humans; D008297:Male; D010784:Photomicrography; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects
D003839:Deoxyadenosines; D002110:Caffeine; C058118:2'-deoxyadenosine