Isolation, purification and characterization of infectious hypodermal and hematopoietic necrosis virus (IHHNV) from penaeidshrimp.
A protocol was developed for the isolation and purification of the infectious hypodermal and hematopoietic necrosis virus (IHHNV) of penaeid shrimps. Cesium chloride-banded virus obtained from three sources of infected shrimps was found to have a buoyant density of 1.33 g/cm3. Also, electron microscopical studies employing negative stain revealed isometric particles having a size of 19 +/- 1 nm. Colorimetric analysis of its nucleic acid type indicated that these particles contain only RNA.
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Department of Microbiology, University of Hawaii, Honolulu.
Loh
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PC
Brock
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JA
D000818:Animals; D002499:Centrifugation, Density Gradient; D033364:Decapoda / Q000382:microbiology*; D008854:Microscopy, Electron; D012367:RNA, Viral / Q000032:analysis; D014780:Viruses / Q000302:isolation & purification* / Q000648:ultrastructure
D012367:RNA, Viral