[Preparative separation of borneol and isoborneol in bingpian by dry-column chromatography].
Borneol (I) and isoborneol (II) in synthetic Bingpian are separated by means of dry-column chromatography (DCC). The sample mixed with a small amount of adsorbent is transferred to the top of the column (50 x 4 cm i.d.) packed with silica gel (200 mesh) and developed with petroleum ether-ethyl acetate-chloroform (20:2: 6) until the solvent has spread from the top to the bottom of column. The zones of I and II can be located by the corresponding Rf of TLC. They are then eluted separately with absolute alcohol. After the concentration of the eluent by vacuum distillation, the crystals of I and II are isolated from the residual solution on standing. The purities of I and II are tested with known specimens by TLC.
Wu
C Y
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Yang
S H
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Wu
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D001892:Camphanes / Q000302:isolation & purification*; D002845:Chromatography / Q000379:methods; D007536:Isomerism
D001892:Camphanes; C022871:isoborneol