Modified method of in vitro cultivation of human keratinocytes suitable for grafting.
The standard method (Green et al., 1979; Simon and Green, 1985) for cultivating human keratinocytes was simplified and adapted to support growth of large keratinocyte sheets (up to 105 X 280 mm) in glass roller bottles. The keratinocyte sheets can be used to cover burn wounds and skin-graft donor sites.
Matousková
E
E
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Praha.
Veselý
P
P
Königová
R
R
D002478:Cells, Cultured; D006801:Humans; D015603:Keratinocytes / Q000637:transplantation*; D016038:Skin Transplantation