title: [Rapid collection of swine lymphocyte subpopulations in large quantity].
abstract: Lymphocyte subpopulations derived from different pig organs (blood, spleen, mesenteric lymph nodes) were separated by a simple, rapid and cheap panning technique, using either normal or ozone treated Petri dishes with bovine serum albumin. Slg+ lymphocytes could thus be obtained with a purity of up to 95% by adhesion onto Corning plastic dishes. The purified cells retained their proliferative activity with regard to lectins. The subpopulation including PT4 and PT8 was then separated by another panning on ozone-treated plastic dishes with a purity of 80-90%.
authors.affiliation: INRA, Centre de Tours, Laboratoire d'immunologie, Nouzilly, France.
authors.forename: B
authors.initials: B
authors.lastname: Charreau
authors.forename: M
authors.initials: M
authors.lastname: Olivier
authors.forename: L
authors.initials: L
authors.lastname: Phan Thanh
authors.forename: H
authors.initials: H
authors.lastname: Salmon
authors.forename: A
authors.initials: A
authors.lastname: Paraf
authors.forename: B
authors.initials: B
authors.lastname: Ruet
authors.forename: D
authors.initials: D
authors.lastname: Sage
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000379:methods; D008214:Lymphocytes / Q000166:cytology*; D013552:Swine / Q000097:blood*
vernacular_title: Obtention rapide de sous-populations lymphocytaires de porc en grande quantité.