Production of antibodies to canine distemper virus in chicken eggs for immunohistochemistry.
The present study describes the application of egg yolk antibodies in immunohistochemistry. In order to obtain specific antibodies against canine distemper virus (CDV), chickens were immunized with attenuated virus. Distinct antibody titres in serum and yolk could be detected by means of a modified plaque/focus immunoassay (ELISA) two weeks after a second immunization. The lower concentrations in corresponding yolk globulin preparations are attributed to the loss of antibodies caused by the isolation procedure (dextran and ammonium sulfate precipitation). After verification of the antibody specificity by indirect immunofluorescence technique high titred globulin fractions were employed in immunohistochemistry using the Avidin-Biotin-Complex method. A specific and distinct immunostaining in formalin fixed and paraffin embedded brain sections of CDV-infected dogs was obtained. The advantages of egg yolk antibodies for immunological purposes are discussed in detail.
Schmidt
P
P
Hafner
A
A
Reubel
G H
GH
Wanke
R
R
Franke
V
V
Lösch
U
U
Dahme
E
E
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000276:immunology*; D002645:Chickens; D004217:Distemper Virus, Canine / Q000276:immunology*; D004530:Egg Yolk; D007136:Immunoglobulins / Q000096:biosynthesis*; D007150:Immunohistochemistry
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007136:Immunoglobulins