Effect of ligustrazine on Ca2+ uptake by inside-out red cell membrane vesicles from renal hypertensive rats.
The effect of ligustrazine (Lig) on blood pressure and Ca2+ uptake by inside-out red cell membrane vesicles (IOV) was investigated in renal hypertensive rats (RHR). After oral administration of Lig (30 mg/kg body weight/day) for 10 days, the blood pressure of RHR was not significantly changed (before vs after experiment: 147.50 +/- 2.50 vs 150.00 +/- 13.42 mmHg). The active Ca2+ uptake rate of IOVs from RHR was 5.03 +/- 1.15 nmol/ng IOV protein/min, and this was lower than that of IOVs from normotensive Wistar rats (8.95 +/- 1.08 nmol/ng IOV protein/min, P less than 0.01). In RHR, no change in IOV Ca2+ uptake capacity was observed after administration of Lig in vivo. Experiments in vitro showed that Lig markedly reduced Ca2+ uptake by IOVs from both RHR and control rats. The results indicate that the active Ca2+ transport capacity of red cell membranes is decreased in RHR and not significantly affected by oral administration of Lig; this drug, however, exerts an inhibitory effect on the transport process in vitro.
Luo
H
H
Wen
Y Y
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Chen
M Q
MQ
D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006977:Hypertension, Renal / Q000378:metabolism / Q000503:physiopathology*; D008297:Male; D011719:Pyrazines / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D002121:Calcium Channel Blockers; D011719:Pyrazines; D002118:Calcium; C017953:tetramethylpyrazine