title: Growth kinetics of four human breast carcinomas grown in nude mice.
abstract: The immune-deficient nude mouse with human tumor xenografts is an appropriate model system for performing detailed growth kinetic examinations. In the present study one estrogen and progesterone receptor-negative (T60) and three receptor-positive (Br-10, MCF-7, T61) human breast cancer xenografts in nude mice were investigated. The proliferative tumor characteristics were examined by growth curves, thymidine labelling technique, and flow cytometric DNA analysis performed on fine-needle aspirations. The results showed that the tumors had growth kinetics comparable to other human tumor types with cell generation times of 42 to 60 hours. The three receptor-positive tumors had slower growth rate, larger tumor volume doubling time, and smaller growth fraction and labelling index than the receptor-negative tumor. However, no single proliferation parameter was sufficient to characterize the growth kinetics of individual tumors or to describe proliferative differences between the tumors.
authors.affiliation: University Institute of Pathological Anatomy, Copenhagen, Denmark.
authors.forename: M
authors.initials: M
authors.lastname: Spang-Thomsen
authors.forename: K
authors.initials: K
authors.lastname: Rygaard
authors.forename: L
authors.initials: L
authors.lastname: Hansen
authors.forename: A C
authors.initials: AC
authors.lastname: Halvorsen
authors.forename: L L
authors.initials: LL
authors.lastname: Vindeløv
authors.forename: N
authors.initials: N
authors.lastname: Brünner
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000032:analysis / Q000473:pathology*; D002453:Cell Cycle; D004273:DNA, Neoplasm / Q000032:analysis; D005434:Flow Cytometry; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D008940:Mitotic Index; D009368:Neoplasm Transplantation; D011960:Receptors, Estrogen / Q000032:analysis*; D011980:Receptors, Progesterone / Q000032:analysis*; D014407:Tumor Cells, Cultured / Q000166:cytology
chemical_list: D004273:DNA, Neoplasm; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone