[Determination of serum lipid peroxidase using 10-N methylcarbamoyl-3,7-dimethylamino-10 H-phenothiazine (MCDP)--with special reference to aging and diseases].
We developed an assay for determining serum lipid peroxide (LPO) using MCDP. LPO levels in 125 healthy middle-aged and 70 elderly subjects. LPO level in the healthy and the elderly subjects was 1.45 +/- 1.37 nmol/ml and 2.1 +/- 3.0 nmol/ml, respectively. LPO did not increase with age. There was no correlation in the LPO levels determined by the MCDP and TBA methods. We determined the LPO distribution in the fractions of lipoprotein prepared by ultracentrifugation. In the LDL fraction of the diseased (myocardial infarction), the LPO level was higher than that of healthy subjects. Periodic measurement of LPO levels in 10 surgically operated patients revealed that the LPO level decreased significantly on the first day after operation. Our assay is easy to use and shows a lower level than the TBA method. LPO measured by this method would represent a substance different from that measured by the TBA method, i.e., MCDP mainly measures hydroperoxides, whereas the TBA method measures endoperoxides.
Yoshimine
N
N
Tateishi
T
T
D000818:Animals; D004285:Dogs; D005609:Free Radicals; D006801:Humans; D008054:Lipid Peroxides / Q000097:blood*; D008297:Male; D008722:Methods; D051379:Mice; D008875:Middle Aged; D010640:Phenothiazines; D051381:Rats
C062307:10-N-methylcarbamoyl-3,7-dimethylamino-10H-phenothiazine; D005609:Free Radicals; D008054:Lipid Peroxides; D010640:Phenothiazines