Liposome-encapsulated hemoglobin as an artificial red blood cell: characterization and scale-up.
Encapsulation methods using high pressure extrusion and homogenization were developed which produce hemoglobin-containing liposomes, approximately one micron or less in diameter with an oxygen-carrying capacity of more than half that of red blood cells (RBCs). These methods were developed for scaling-up liposome-encapsulated hemoglobin (LEH) production. Previously, the lack of adequate scale-up methods has been a serious barrier to full scale efficacy and toxicity testing for all the researchers engaged in such investigations. The scale-up methods and characterization of the resulting LEH preparations are presented.
Vidal-Naquet
A
A
Department of Chemical Engineering, Illinois Institute of Technology, Chicago.
Gossage
J L
JL
Sullivan
T P
TP
Haynes
J W
JW
Gilruth
B H
BH
Beissinger
R L
RL
Sehgal
L R
LR
Rosen
A L
AL
D001802:Blood Substitutes; D004337:Drug Carriers; D004339:Drug Compounding; D004912:Erythrocytes; D006454:Hemoglobins / Q000008:administration & dosage*; D006801:Humans; D008081:Liposomes; D010100:Oxygen / Q000378:metabolism; D010316:Particle Size; D010743:Phospholipids
D001802:Blood Substitutes; D004337:Drug Carriers; D006454:Hemoglobins; D008081:Liposomes; D010743:Phospholipids; D010100:Oxygen