title: Measurement of K vitamins in animal tissues by high-performance liquid chromatography with fluorimetric detection.
abstract: A highly sensitive method for measuring endogenous phylloquinone and menaquinones in animal tissues was developed, based on high-performance liquid chromatography with coulometric reduction and fluorimetric detection, following extraction from tissue homogenate and purification on a Sep-Pak silica cartridge followed by thin-layer chromatography. The detection limits of phylloquinone, menaquinone-4, -6, -10 and -13 were 40, 40, 50, 70 and 80 pg/g in rat liver, respectively.
authors.affiliation: Shionogi Research Laboratories, Shionogi & Co, Ltd., Osaka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hirauchi
authors.forename: T
authors.initials: T
authors.lastname: Sakano
authors.forename: S
authors.initials: S
authors.lastname: Notsumoto
authors.forename: T
authors.initials: T
authors.lastname: Nagaoka
authors.forename: A
authors.initials: A
authors.lastname: Morimoto
authors.forename: K
authors.initials: K
authors.lastname: Fujimoto
authors.forename: S
authors.initials: S
authors.lastname: Masuda
authors.forename: Y
authors.initials: Y
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D013050:Spectrometry, Fluorescence; D014018:Tissue Distribution; D014812:Vitamin K / Q000032:analysis* / Q000493:pharmacokinetics
chemical_list: D014812:Vitamin K