Interspecies relationship of a repetitive chromosome-specific DNA.
A repetitive DNA motif, consisting of 48 bp units (D22Z3) was shown to reside in the pericentric region of the human chromosome 22. In genomic blots it was displaying cross-homology only to great ape species, the restriction patterns being similar but specific for each species investigated. Non-radioactive in situ hybridization revealed a minor homology to human chromosomes 14/15 when less stringent conditions were applied. In gorilla, only chromosomes corresponding to human G-group autosomes showed a distinct cross-hybridization, whereas in chimpanzee, homology to this sequence was observed for all acrocentric chromosomes, even with reduced signal accumulation for the human 22 equivalent at higher stringencies.
Scherthan
H
H
Lutz
S
S
Metzdorf
R
R
Blin
N
N
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D004274:DNA, Recombinant; D006801:Humans; D009693:Nucleic Acid Hybridization; D002679:Pan troglodytes / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid
D004274:DNA, Recombinant