Characterization of age-related malondialdehyde oxidation: the effect of modulation by food restriction.
Age-related in vitro malondialdehyde (MDA) oxidation by liver was investigated. It was established that mitochondria are the major site of oxidation, and that the oxidation process was catalyzed by aldehyde dehydrogenase as indicated by inhibitory action of disulfiram. The reaction of MDA oxidation requires Mg2+ and NAD as cofactors. The capacity for MDA oxidation was progressively lost with increasing age and the extent of the loss was modulated by food restriction.
Kim
J W
JW
Department of Physiology, University of Texas, San Antonio 78284-7756.
Yu
B P
BP
D000375:Aging / Q000378:metabolism*; D000818:Animals; D004221:Disulfiram / Q000494:pharmacology; D005508:Food Deprivation / Q000502:physiology*; D008099:Liver / Q000378:metabolism; D008297:Male; D008314:Malonates / Q000378:metabolism*; D008315:Malondialdehyde / Q000032:analysis / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D051381:Rats; D011916:Rats, Inbred F344; D013347:Subcellular Fractions / Q000032:analysis
D008314:Malonates; D009243:NAD; D008315:Malondialdehyde; D004221:Disulfiram