Cytologic characteristics of neoplastic and of regenerating hepatocytes in fine needle aspirates of rat liver.
L-azaserine (l-aza)-induced hepatocellular carcinoma (HCA) and hyperplastic liver lesions were studied in livers from 86 l-aza treated animals and 16 saline controls. Eight additional rats studied 24 or 48 hr after partial hepatectomy and 8 young rats studied after no experimental manipulation served as additional controls for determination of cytologic effects of increased cell turnover. Fine needle aspirates (FNA) were made of all gross liver lesions and of grossly normal liver; H&E stained sections of liver surrounding each needle track were made. The FNA were fixed in 95% ethanol, stained with a routine Papanicolaou stain, randomized, and evaluated using 18 cytologic features. They were diagnosed without knowledge of the histologic diagnosis or treatment group. Features were analyzed with discriminant function analysis to determine their relative importance in making the diagnosis. FNA proved to be an accurate method for diagnosis of hepatocellular lesions in laboratory animals. The cytologic features of greatest usefulness in establishing the diagnosis were cord pattern, chromatin pattern, nucleolar size and nuclear membrane irregularity. FNA could provide a useful experimental technique for following evolving lesions serially and for identifying subgroups within hyperplastic nodules.
Giampaolo
C
C
Mercy Hospital and Medical Center, Chicago, Illinois 60616-2477.
Brady
K
K
Kowalski
B
B
Rogers
A E
AE
D000818:Animals; D001377:Azaserine; D001707:Biopsy, Needle; D002466:Cell Nucleolus / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure; D003593:Cytoplasm / Q000648:ultrastructure; D008099:Liver / Q000166:cytology* / Q000473:pathology / Q000648:ultrastructure; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000473:pathology* / Q000648:ultrastructure; D008115:Liver Regeneration; D008297:Male; D009685:Nuclear Envelope / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains
D001377:Azaserine