2-Deoxy-D-glucose induced modification of chromosomal damage in UV-irradiated peripheral human leukocytes.
UV-irradiation (0.6 J/m2) of peripheral human leukocytes 27 hr after PHA-stimulation induced a considerable mitotic delay in the cultures. Approximately two thirds of the chromosomal aberrations induced by UV were gaps of the chromatid and isochromatid types. Treatment with glucose antimetabolite 2-deoxy-D-glucose (2-DG) alone did not induce any chromosomal damage. Presence of 2-DG (5 mM, equimolar with glucose) for 2 hr after UV-irradiation resulted in a significant reduction in the frequency of cells with aberrations. Decrease in the total aberrations per cell was also observed. The data are consistent with earlier observations that 2-DG reduces the manifestation of radiation damage in normal proliferating cells.
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D000328:Adult; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D003837:Deoxy Sugars / Q000494:pharmacology*; D003847:Deoxyglucose / Q000494:pharmacology*; D006801:Humans; D007962:Leukocytes / Q000166:cytology / Q000528:radiation effects; D008297:Male; D008938:Mitosis / Q000528:radiation effects*; D010835:Phytohemagglutinins / Q000494:pharmacology; D014466:Ultraviolet Rays
D003837:Deoxy Sugars; D010835:Phytohemagglutinins; D003847:Deoxyglucose