Chromosomal mapping and nucleotide sequence of a human DNA autonomously replicating sequence.
A 1.1-kb human DNA fragment (ARSH1) capable of functioning as a putative origin of replication in yeast cells has been characterized both by in situ hybridization to human metaphase chromosomes and by DNA sequencing. Our hybridization studies show a preferential localization of ARSH1 in chromosome regions 1p34-36 and 2q34-37. DNA sequence analysis indicates that in addition to the consensus sequence required for ARS function in yeast cells, nuclear matrix-associated DNA motifs are also present in the 1.1-kb fragment. These results suggest that ARSH1 sequences may serve as points of anchorage to the nuclear matrix for chromosomes 1 and 2.
Aguinaga
M
M
Division of Biomedical Sciences, Meharry Medical College, Nashville, Tennessee 37208.
Sharan
C
C
Singh
D N
DN
Kegler
D
D
Siddiqui
K A
KA
Valenzuela
M S
MS
D000595:Amino Acid Sequence; D001483:Base Sequence; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D002889:Chromosomes, Human, Pair 2; D004247:DNA / Q000235:genetics*; D004261:DNA Replication; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D015003:Yeasts / Q000235:genetics
D004247:DNA