[A modified method of preserving feces for the detection of intestinal protozoa].
Methylene blue is excluded from the original preservative in the modified conservation method elaborated by Safaraliev. The conserved material is examined in humid smear or, still better, after enrichment by falling out in the formol-ether solution or staining the smears by Lugol's solution or 0.25% solution of methylene blue. Intestinal Protozoa may be kept conserved for no less than a year. In case of enrichment the technique becomes 2--3-fold more effective than the original one, enabling to save the reagents, laboratory vessels, time (for microscopy), as well as to evade the odour of acetic acid and phenol from the preparations under study.
Safaraliev
R S
RS
Mamedova
F M
FM
Abuzarov
R M
RM
Abdullaeva
L A
LA
Avshalumova
L L
LL
D000818:Animals; D056890:Eukaryota / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology*; D006801:Humans; D007422:Intestines / Q000469:parasitology*; D011309:Preservation, Biological / Q000379:methods*
Modifitsirovannyĭ metod konservirovaniia fekaliĭ dlia vyiavleniia prosteĭshikh kishechnika.