title: Pan natural killer cell monoclonal antibodies and their relationship to the NK1.1 antigen.
abstract: The study of natural killer (NK) has been difficult because they account for a small percentage of peripheral blood and splenic lymphocytes and the paucity of NK specific antigens that have been identified. We have isolated pure populations of C57BL/6 (H-2b) NK cells using the IgG2b monoclonal antibody PK136 (anti-NK1.1). These NK1.1+ cells were used to immunize 129/J (H-2b) mice, and in this report, we describe three new NK specific monoclonal antibodies (SW3A4(IgM), SW4B12(IgG1), and SW2B4(IgG2b] and their relationship to the known murine NK antigen NK1.1. We have further characterized the NK1.1 antigen as a 39 kd molecule which is coded for by a gene which appears to map to chromosome 6.
authors.affiliation: Department of Pathology and Immunology Graduate Program, University of Texas Southwestern Medical Center, Dallas.
authors.forename: C L
authors.initials: CL
authors.lastname: Sentman
authors.forename: J
authors.initials: J
authors.lastname: Hackett
authors.forename: T A
authors.initials: TA
authors.lastname: Moore
authors.forename: M M
authors.initials: MM
authors.lastname: Tutt
authors.forename: M
authors.initials: M
authors.lastname: Bennett
authors.forename: V
authors.initials: V
authors.lastname: Kumar
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000950:Antigens, Ly / Q000302:isolation & purification; D002469:Cell Separation; D007694:Killer Cells, Natural / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D013045:Species Specificity; D014407:Tumor Cells, Cultured / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000950:Antigens, Ly