Determination of drug protein-binding by high-performance liquid chromatography using a chemically bonded bovine albumin stationary phase.
A liquid chromatographic method for the determination of the degree of protein-binding of drugs has been established, using a stationary phase to which bovine serum albumin has been bonded chemically. In a structurally heterogeneous group of compounds, results of the method correlate well with protein-binding data obtained by equilibrium dialysis (r = 0.89, n = 23, p less than 0.001). Within a series of analogous piperazines a good correlation is found (r = 0.981, n = 11, p less than 0.001). The chromatographic method allows automation of the measurement of protein-binding of large series of compounds with protein-binding ranging between 10 and 99%. The method is not expensive and is less time consuming than equilibrium dialysis. Only 1 mg of technical-grade material is required to determine the protein-binding, and radioactive labelling of the material is not necessary.
Lammers
N
N
Duphar B.V., Analytical Development Department, DA Weesp, The Netherlands.
De Bree
H
H
Groen
C P
CP
Ruijten
H M
HM
de Jong
B J
BJ
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D010879:Piperazines / Q000378:metabolism; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D012710:Serum Albumin, Bovine; D013056:Spectrophotometry, Ultraviolet; D013424:Sulfanilamides / Q000378:metabolism
D004364:Pharmaceutical Preparations; D010879:Piperazines; D011506:Proteins; D013424:Sulfanilamides; D012710:Serum Albumin, Bovine