Analysis of gene amplification in human tumor cells by in-gel DNA renaturation assay.
The incidence of gene amplification in leukemia/lymphoma is significantly lower than that in solid tumors. For understanding the biological characteristics of leukemia/lymphoma, it is necessary to clarify whether the incidence of gene amplification is actually lower or gene amplification specific to hemopoietic malignancies has not yet been found. We describe a new version of in-gel DNA renaturation technique for general screening of gene amplification that allows detection and cloning of amplified genes of unknown nature as few as 7- to 8-fold amplification.
Fukumoto
M
M
Tashiro
H
H
Roninson
I B
IB
D004273:DNA, Neoplasm / Q000235:genetics*; D005782:Gels; D005784:Gene Amplification / Q000235:genetics*; D006801:Humans; D009695:Nucleic Acid Renaturation; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D005782:Gels