Platelet mobilization induced by PAF and its role in the thrombocytosis triggered by adrenaline in rats.
The injection of PAF (6 micrograms/kg, i.v.) induced, in rats, haemoconcentration accompanied by an increase in the platelet number, as attested by the counts of platelets in blood samples diluted in formalin-free EDTA solution. This increase was significant at 15 min, peaked from 1 to 4 h and returned to basal levels 24 h after the lipid administration. The release of platelets induced by PAF was inhibited dose-dependently by specific PAF receptor antagonist such as WEB 2086 (0.5-2 mg/kg), BN 52021 and 48740 RP (5-25 mg/kg). Furthermore, platelet mobilization was clearly impaired in splenectomized animals stimulated by PAF, whereas thrombocytopenia and haemoconcentration by the same stimulus were intact. It was also noted that a second injection of PAF, 24 h after the initial stimulation with the lipid, failed to induce an increase in platelet counts, indicating autodesensitization. Desensitization to PAF or pretreatment with PAF antagonists clearly prevented the increase in the platelet counts after stimulation by adrenaline (15 micrograms/kg). These findings suggest that, in rats, PAF can induce release of platelets by a spleen-dependent mechanism and that this lipid may be relevant to the thrombocytosis triggered by adrenaline.
Castro-Faria-Neto
H C
HC
Departamento de Fisiologia e Farmacodinâmica, Fundaçáo Oswaldo Cruz, Rio de Janeiro, Brazil.
Bozza
P T
PT
Martins
M A
MA
Dias
P M
PM
Silva
P M
PM
Cordeiro
R S
RS
D000818:Animals; D004837:Epinephrine / Q000494:pharmacology*; D006400:Hematocrit; D008297:Male; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000502:physiology*; D010976:Platelet Count / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000502:physiology; D013156:Splenectomy; D013922:Thrombocytosis / Q000097:blood* / Q000139:chemically induced
D010972:Platelet Activating Factor; D004837:Epinephrine