Dual effect of a protein-free hemodialysate on the oxygen uptake of phagocytosing human polymorphonuclear leucocytes.
The effect of a protein-free hemodialysate (Solcoseryl) on the oxygen uptake of human polymorphonuclear leucocytes (PMN) was studied. No effect was observed on resting PMN over a concentration range from 0.5 to 10% v/v. Instead, when PMN phagocytosing serum opsonized zymosan (SOZ) were used, low concentrations (0.5-2.5% v/v) of the hemodialysate increased their oxygen uptake, whereas higher concentrations (5-10% v/v) were inhibitory. At a concentration of 10% the hemodialysate was shown to inhibit also SOZ particles uptake. These findings suggest that inhibition of the oxygen uptake might be related to inhibition of phagocytosis. The mechanism of the stimulatory effect observed at low concentrations remains to be established but it does not seem to be related to a direct activation of the biochemical pathway/s linked to O2 consumption since the hemodialysate per se had no effect on the respiration of resting PMN. The results are discussed in the light of the known clinical effects of the hemodialysate.
Dri
P
P
Istituto di Patologia Generale, Università di Trieste, Italy.
Cramer
R
R
Mittenzwei
H
H
Patriarca
P
P
D000183:Actihaemyl / Q000494:pharmacology*; D015314:Dialysis Solutions / Q000494:pharmacology*; D015312:Hemodialysis Solutions / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide; D066298:In Vitro Techniques; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D009895:Opsonin Proteins; D010101:Oxygen Consumption / Q000187:drug effects*; D010587:Phagocytosis / Q000187:drug effects*; D011048:Polarography; D014020:Tissue Extracts / Q000494:pharmacology*; D015054:Zymosan / Q000378:metabolism
D015314:Dialysis Solutions; D015312:Hemodialysis Solutions; D009895:Opsonin Proteins; D014020:Tissue Extracts; D000183:Actihaemyl; D015054:Zymosan; D006861:Hydrogen Peroxide