[Purification of human thrombin by affinity chromatography for its use in preparations for biological coagulation].
Biological glue is obtained by mixing different specific plasma proteins including a serine protease, thrombin. Surprisingly at present the thrombin used in such a mixture is from equine or bovine origin while all other components are from human. In this paper we described a particular efficient and specific chromatographic method for the purification of human thrombin usable as a serine protease in the preparation of biological glue. A pure and active thrombin is obtained from a plasma fraction after adsorption on benzamidine-Spherodex followed by an elution with non specific (sodium chloride gradient) or biospecific competitors (arginine methylester or benzamidine). The obtained thrombin with a yield close to 80% and a purification factor close to 160, showed good properties in the replacement of animal thrombin in the condition of biological glue.
Lorne
J L
JL
Centre de Transfusion sanguine des Armées "Jean-Julliard" (CTSA), Clamart.
Allary
M
M
Boschetti
E
E
D001550:Benzamidines; D001777:Blood Coagulation; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006487:Hemostasis; D006801:Humans; D011516:Prothrombin / Q000378:metabolism; D013917:Thrombin / Q000302:isolation & purification* / Q000378:metabolism
D001550:Benzamidines; D011516:Prothrombin; D013917:Thrombin
Purification de la thrombine humaine par chromatographie d'affinité en vue de son utilisation dans les préparations de colle biologique.