The correlation between molecular weight and antitumor activity of galactosaminoglycan (CO-N) from Cordyceps ophioglossoides.
A galactosaminoglycan (CO-N) obtained by ultrasonication from a protein-bound polysaccharide SN-C, which was isolated from Cordyceps ophioglossoides culture, has a direct cytotoxicity against tumor cells (Ohmori et al., Chem. Pharm. Bull., 37, 1019 (1989). High performance liquid chromatographic analysis revealed that CO-N shows a broad molecular weight distribution with an average molecular weight of 33000. A potent antitumor activity of CO-N was observed in the higher-molecular-weight fraction on gel filtration, and the low-molecular-weight fraction below 6600 showed a weak activity. However, the depolymerized CO-N (ca. 5500) obtained by further ultrasonication of the original CO-N still retained the antitumor activity of CO-N against Ehrlich ascitic carcinoma or MM46 solid mammary carcinoma.
Ohmori
T
T
Tamura
K
K
Ohgane
N
N
Nakamura
T
T
Kawanishi
G
G
Yamada
H
H
Nomoto
K
K
D000818:Animals; D000970:Antineoplastic Agents; D006999:Hypocreales / Q000737:chemistry*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008813:Mice, Inbred ICR; D008970:Molecular Weight; D011134:Polysaccharides / Q000032:analysis* / Q000494:pharmacology
D000970:Antineoplastic Agents; D011134:Polysaccharides; C007815:galactosaminoglycan