Physical and genetic mapping of the protein A gene in the chromosome of Staphylococcus aureus 8325-4.
The gene coding for protein A (spa) has been mapped close to nov on the genetic map of the chromosome of Staphylococcus aureus 8325-4. A rapid mapping procedure has been developed which first allowed the region of the chromosome carrying the spa gene to be identified by blot +hybridization of large DNA fragments which had been separated by pulsed-field gel electrophoresis. Restriction endonuclease SmaI fragment G was shown to carry the spa gene. An insertion mutation in spa was constructed by in vitro insertion of a fragment of DNA expressing resistance to kanamycin and neomycin. A spa::Kan(r)Neo(r) mutation was isolated in S. aureus 8325-4 by allele replacement. This provided a selectable marker which allowed the spa gene to be mapped by transformation analysis.
Patel
A H
AH
Department of Microbiology, Iowa State University, Ames 50011.
Foster
T J
TJ
Pattee
P A
PA
D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D011994:Recombinant Proteins / Q000235:genetics; D013205:Staphylococcal Protein A / Q000235:genetics*; D013211:Staphylococcus aureus / Q000235:genetics* / Q000472:pathogenicity; D014169:Transformation, Bacterial; D014774:Virulence
D004269:DNA, Bacterial; D011994:Recombinant Proteins; D013205:Staphylococcal Protein A