A comparative study of bark lectins from three elderberry (Sambucus) species.
Three elderberry lectins isolated from the bark of three different species of the genus Sambucus which are native to Europe (S. nigra), North America (S. canadensis), and Japan (S. sieboldiana) were studied comparatively with regard to their carbohydrate binding properties and some structural features. All three lectins contained two identical carbohydrate binding sites per molecule and showed a very high specificity for the Neu5Ac(alpha 2-6)-Gal/GalNAc sequence. However, relative affinities for various oligosaccharides were significantly different among them, suggesting differences in the detailed structure of the carbohydrate binding sites of these lectins. The three lectins were immunologically related, but not identical, and all were composed of hydrophobic and hydrophilic subunit regions, although the molecular sizes of these subunits were slightly different among the three lectins. N-terminal sequence analysis of the subunits of these lectins suggested that they have a very similar structure in this region but also indicated the occurrence of N-terminal processing such as the deletion of several amino acid residues at the N-termini for both hydrophobic and hydrophilic subunits of all three lectins. Tryptic peptide mapping of the three lectins showed a similar pattern for all of them but also showed the presence of some unique peptides for each lectin.
Shibuya
N
N
National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries, Ibaraki.
Tazaki
K
K
Song
Z W
ZW
Tarr
G E
GE
Goldstein
I J
IJ
Peumans
W J
WJ
D000595:Amino Acid Sequence; D001665:Binding Sites; D050260:Carbohydrate Metabolism; D003956:Dialysis; D004591:Electrophoresis, Polyacrylamide Gel; D037102:Lectins / Q000378:metabolism*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010449:Peptide Mapping; D037121:Plant Lectins; D010944:Plants / Q000378:metabolism; D054788:Ribosome Inactivating Proteins; D014316:Tritium; D014357:Trypsin / Q000378:metabolism
D037102:Lectins; D037121:Plant Lectins; C050600:Sambucus nigra lectins; D014316:Tritium; D054788:Ribosome Inactivating Proteins; D014357:Trypsin