Detection of low-Mr glycoproteins in surface washes of some fungal cultures by gel-filtration HPLC and by monoclonal antibodies.
Surface washings were prepared from cultures of fungi grown on agar slants and the wash components were fractionated by gel-filtration HPLC. A range of low-Mr glycoproteins (less than 10,000) were detected in all the washes, and in some species several high-Mr glycoprotein components (greater than 150,000) were also present. The HPLC analyses indicated species-specific differences in the profiles. Regions of specific antigenicity within profiles were detected by monoclonal antibodies.
MacDonald
M M
MM
Department of Plant Sciences, University of Oxford, UK.
Dunstan
R H
RH
Dewey
F M
FM
D000911:Antibodies, Monoclonal; D000946:Antigens, Fungal / Q000032:analysis; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005656:Fungal Proteins / Q000032:analysis*; D005658:Fungi / Q000276:immunology*; D006023:Glycoproteins / Q000032:analysis*
D000911:Antibodies, Monoclonal; D000946:Antigens, Fungal; D005656:Fungal Proteins; D006023:Glycoproteins