Isolation of timothy (Phleum pratense) allergens using affinity chromatography with monoclonal antibodies.
The monoclonal antibody 1 D11, directed against the timothy pollen allergens I, II, III, and a further, anodically migrating component, is suitable for the isolation of these allergens by means of affinity chromatography. The purified allergens consist of glycoproteins with many different isoelectric points. Their molecular weights were estimated as approximately the same as those of the allergens in the whole timothy pollen extract. Their allergenic activity (IgE binding) was not lost during the purification steps.
Jung
K
K
Institute of Clinical Immunology, Friedrich Schiller University, Jena.
Fahlbusch
B
B
Müller
W D
WD
Herrmann
D
D
Diener
C
C
Jäger
L
L
D000485:Allergens / Q000302:isolation & purification*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002846:Chromatography, Affinity; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D007525:Isoelectric Focusing; D008970:Molecular Weight; D010940:Plant Proteins / Q000276:immunology / Q000302:isolation & purification; D006109:Poaceae / Q000276:immunology*; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology
D000485:Allergens; D000911:Antibodies, Monoclonal; D010940:Plant Proteins