title: Production of parathyroid hormone-related protein by a rat parathyroid cell line.
abstract: Parathyroid hormone-related protein (PTHrP), the peptide associated with humoral hypercalcemia of malignancy, has been identified in fetal and adult parathyroid glands. We here report a sub-clone of a rat parathyroid cell line which secretes a single peptide species corresponding in size to PTHrP(1-84). Biological activity of the secretion product was blocked by a specific antiserum against PTHrP, but not by parathyroid hormone (PTH) antiserum. Secretion of PTHrP by these cells was regulated by extracellular calcium in the physiological range. A single messenger RNA species for PTHrP was identified, though PTH mRNA could not be shown in these cells. Hybrid CAT genes containing 700-1000 bp of 5'-flanking DNA from the human PTH or PTHrP genes were transfected into these cells, and the PTHrP gene was expressed at 10-fold higher levels than the PTH gene. These cells thus provide a valuable model system for investigation expression of PTHrP in a non-transformed cell line.
authors.affiliation: Department of Medicine, Royal Melbourne Hospital, University of Melbourne, Victoria, Australia.
authors.forename: J D
authors.initials: JD
authors.lastname: Zajac
authors.forename: J
authors.initials: J
authors.lastname: Callaghan
authors.forename: C
authors.initials: C
authors.lastname: Eldridge
authors.forename: H
authors.initials: H
authors.lastname: Diefenbach-Jagger
authors.forename: L J
authors.initials: LJ
authors.lastname: Suva
authors.forename: P
authors.initials: P
authors.lastname: Hudson
authors.forename: J M
authors.initials: JM
authors.lastname: Moseley
authors.forename: V P
authors.initials: VP
authors.lastname: Michelangeli
authors.forename: G
authors.initials: G
authors.lastname: Pasquini
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002460:Cell Line; D005786:Gene Expression Regulation; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D010280:Parathyroid Glands / Q000166:cytology / Q000378:metabolism*; D010281:Parathyroid Hormone / Q000096:biosynthesis; D044162:Parathyroid Hormone-Related Protein; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis; D014162:Transfection
chemical_list: D009363:Neoplasm Proteins; D010281:Parathyroid Hormone; D044162:Parathyroid Hormone-Related Protein; D012333:RNA, Messenger; D011994:Recombinant Proteins; D000262:Adenylyl Cyclases; D002118:Calcium