Preparative resolution of enantiomers of prostaglandin precursors by liquid chromatography on a chiral stationary phase.
Preparative liquid chromatographic methods were developed for the chiral resolution of two different cyclopentenone precursors of a synthetic prostaglandin. Various solvent combinations of alcohols and alkanes were investigated to determine the method which has the greatest throughput. The effect of particle size on the chiral resolution was also investigated. In addition, the preparative system was automated to allow for unattended operation for up to 10 h.
Miller
L
L
Chemical Development Department, G.D. Searle and Co., Skokie, IL 60077.
Bush
H
H
D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D011453:Prostaglandins / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D011453:Prostaglandins