[High yielding culture of LAK cells by the concentration rotary tissue culture system and its clinical application].
The concentration rotary tissue culture system (Kawasumi Laboratories, Inc. Japan) was utilized to induce LAK cells from the peripheral blood lymphocytes (PBLs) of brain tumor patients. These LAK cells were administrated into the tumor cavity or cerebrospinal space of the patients. Under our culture system, the final administration of LAK cells increased tenfold of the initial PBLs, which were collected by leukapheresis. Around 4 weeks after the culture, these cells could not increase in number, with the decrease in cytotoxicity activity against Daudi and human glioblastoma (ONS-12) cells. The level of ammonium and lactate in the culture medium were comparatively kept low. IL-2 receptors were amplified with the increase in T cell population, especially helper T cells. This system may be a good tool to induce LAK cells for adoptive immunotherapy.
Park
K C
KC
Shimizu
K
K
Tamura
K
K
Yamada
M
M
Matsui
Y
Y
Mabuchi
E
E
Moriuchi
S
S
Mogami
H
H
D000328:Adult; D001932:Brain Neoplasms / Q000276:immunology* / Q000628:therapy*; D002648:Child; D002675:Child, Preschool; D046508:Culture Techniques / Q000379:methods; D005260:Female; D006801:Humans; D007116:Immunization, Passive; D015979:Killer Cells, Lymphokine-Activated / Q000166:cytology / Q000276:immunology; D008297:Male