Tuberculin sensitivity and an interaction of leucocyte and plasma factors involving fibrinogen.
Lysates of blood leucocytes from strong tuberculin-positive reactors contained smaller particles of insoluble protein-rich material than did similar preparations from negative donors. The phenomenon was dependent on the presence of small quantities of plasma in the original leucocyte suspensions: when this was removed or replaced by serum both groups of donors produced particles of the smaller size. When the experiments were repeated in the presence of 125I-fibrinogen, larger particle size was found to be associated with an increased proportion of radioactivity becoming firmly bound to the insoluble material. These findings are likely to reflect differences in the levels of procoagulant, proteolytic enzymes or associated activities in the cells or plasma of the two groups.
Salaman
M R
MR
Department of Immunology, St. Mary's Hospital Medical School, London, UK.
Robinson
J
J
Rampling
M W
MW
D000328:Adult; D003851:Deoxyribonucleases / Q000378:metabolism; D005260:Female; D005340:Fibrinogen / Q000378:metabolism*; D005615:Freezing; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007962:Leukocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D014373:Tuberculin / Q000276:immunology
D014373:Tuberculin; D005340:Fibrinogen; D003851:Deoxyribonucleases