[Changes in the pH of leukocyte granules during the storage of preserved donor blood].
Data are presented on pH values in lymphocyte and neutrophil granules, as well as parameters of fluctuating pH changes in individual granules at early terms of storage (up to 72 h), evaluated by cytophotometry with the use of the vital stain neutral red in microspectrophotometer (Univar Reichert, Austria). An average value of pH in donor neutrophil granules was 6.57-0.12 (range 6.21-7.02). At day 3 of storage it decreased at average by 0.18 pH units. Rhythmical changes of pH in individual granules with pH 0.2-0.44 were found on all days of storage. The period of the rhythm lengthened from 3 min on day 1 to 4 min on day 3. This shift as well as acidification of granules during storage reflects their functional deterioration. The storage did not affect pH values in lymphocyte lysosomes which at average were 6.4, 6.5 and 6.35 on days 1, 2 and 3, respectively.
Shestova
O L
OL
Kotel'nikov
V M
VM
Kozinets
G I
GI
D000328:Adult; D001782:Blood Donors; D001793:Blood Preservation; D015925:Cryopreservation; D003592:Cytophotometry; D003594:Cytoplasmic Granules / Q000502:physiology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007962:Leukocytes / Q000502:physiology*; D008297:Male; D015591:Microspectrophotometry; D009499:Neutral Red; D013997:Time Factors
D009499:Neutral Red
Izmenenie pH granul leĭkotsitov v protsesse khraneniia konservirovannoĭ donorskoĭ krovi.