Cytolytic activities of benfluron metabolites and loss of transplantability.
The cytolytic activity of two benfluron metabolites, namely 7-dihydrobenfluron (DBF) and benfluron N-oxide (NOBF), has been studied using both Ehrlich ascites carcinoma (EAC) and P388 leukaemia cells. DBF showed a considerable cytolytic activity (activity of lactate and malate dehydrogenases, release of protein from the cells into the culture medium, morphological changes) particularly at higher concentrations. The highest concentrations caused a significant prolongation of the survival time of the experimental animals.
Miko
M
M
Department of Microbiology and Biochemistry, Slovak Polytechnic University, Bratislava, Czechoslovakia.
Krepelka
J
J
Melka
M
M
D000818:Animals; D000970:Antineoplastic Agents; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy; D002462:Cell Membrane / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D005449:Fluorenes / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007941:Leukemia P388 / Q000188:drug therapy; D008291:Malate Dehydrogenase / Q000378:metabolism; D051379:Mice; D013053:Spectrophotometry; D013997:Time Factors; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D005449:Fluorenes; C059231:7-dihydrobenfluron; C059230:benfluron N-oxide; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase