Determination of clavulanic acid by a sensitive HPLC method.
A new HPLC method is described for the estimation of clavulanic acid. It should be of use for further studies on the penetration of clavulanic acid into body tissues and fluids. It utilizes standard HPLC equipment and UV detection, but provides at least ten-fold increased sensitivity (less than 0.008 mg/l) over previously published methods by using solid phase extraction with imidazole derivatization and subsequent pre-concentration of the sample, and a microbore chromatographic column with a conventional detection system.
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Robert Gordon's Institute of Technology, City Hospital, Aberdeen, UK.
Taylor
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RB
Gould
I M
IM
D002851:Chromatography, High Pressure Liquid; D002969:Clavulanic Acids / Q000032:analysis*; D006801:Humans; D012463:Saliva / Q000032:analysis
D002969:Clavulanic Acids