Sublethal damage repair and radiosensitivity of human squamous cell carcinoma cells grown with different culture techniques.
In this study, cells of a human squamous cell carcinoma line, HN-1, were grown in monolayers and as multicellular tumour spheroids (MTS). Repair of radiation-induced damage was studied by irradiation with single and split doses of X rays (4-8 Gy). It was shown that the amount of sublethal damage that was repaired in this dose range was equal in cells growing in monolayers and as MTS. The radiosensitivity of spheroids, as expressed by spheroid "cure" dose, increased with increasing MTS diameter. It is postulated that, in MTS with no signs of hypoxia, radioresistance diminishes when MTS increase in diameter.
Schwachöfer
J H
JH
Department of Radiotherapy, University Hospital, Nijmegen, The Netherlands.
Crooijmans
R P
RP
van Gasteren
J
J
Hoogenhout
J
J
Jerusalem
C R
CR
Kal
H B
HB
D002294:Carcinoma, Squamous Cell / Q000532:radiotherapy*; D002455:Cell Division / Q000528:radiation effects; D004260:DNA Repair; D006801:Humans; D008722:Methods; D011836:Radiation Tolerance; D014407:Tumor Cells, Cultured / Q000528:radiation effects*