Protein-gold complexes as neuronal markers for long-term tracing studies.
In this study, we have tested the possible use of protein-gold complexes as neuronal markers for long-term tracing studies in rat. The tracer we have used consisted of colloidal gold particles coupled to wheat-germ agglutinin apohorseradish peroxidase conjugate (WGA-apoHRP). The neuronal labeling was studied for survival periods of up to nineteen months following injection in the central nervous system. Maximal visualization of the gold particles was achieved through gold silver intensification. The tracer could be detected throughout the entire range of periods considered. The injection site consisted of a dense black core and retrogradely labeled cells were characterized by round black granules over the cell body. The retrogradely labeled cells were cytochemically characterized by demonstrating their transmitter content. Thus protein-gold complexes may be used as long-term neuronal markers compatible with the persistance of the vital functions of the labeled cells.
Menétrey
D
D
INSERM, Physiopharmacologie du système nerveux, Paris, France.
de Pommery
J
J
D000818:Animals; D001692:Biological Transport; D005629:Frozen Sections; D006735:Horseradish Peroxidase / Q000008:administration & dosage / Q000493:pharmacokinetics*; D007150:Immunohistochemistry; D007278:Injections, Spinal; D009474:Neurons / Q000378:metabolism*; D010544:Peroxidases / Q000493:pharmacokinetics*; D051381:Rats; D011919:Rats, Inbred Strains; D012012:Red Nucleus / Q000378:metabolism; D013003:Somatosensory Cortex / Q000378:metabolism; D013997:Time Factors; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate; D014909:Wheat Germ Agglutinins / Q000008:administration & dosage / Q000493:pharmacokinetics*
D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate; D014909:Wheat Germ Agglutinins; C062844:wheat germ agglutinin-apohorseradish peroxidase conjugate gold complex; D006735:Horseradish Peroxidase; D010544:Peroxidases