[Study of the effect of etoposide on the fluidity of dipalmitoylphosphatidylcholine liposome by differential scanning calorimetry and Raman spectroscopy].
The interaction of the anticancer drug, etoposide (VP16-213), with DPPC bilayer vesicles was investigated by DSC and Laser Raman techniques. The results present the fact that the incorporation of VP16-213 into aqueous dispersion of DPPC not only shifted the temperatures of phase transition towards higher value but also broadened the half-height width. The Raman spectra of the DPPC liposome in the C-H symmetric strech vibration lost intensity near 2850cm-1, therefore, it can be predicted that VP16-213 is localized at C1-C9 methylene region of the bilayer, which leads to the increase of the membrane's regularity and the decrease of its fluidity.
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D015060:1,2-Dipalmitoylphosphatidylcholine; D002151:Calorimetry / Q000379:methods; D004347:Drug Interactions; D005047:Etoposide; D008081:Liposomes; D008560:Membrane Fluidity; D013059:Spectrum Analysis, Raman
D008081:Liposomes; D015060:1,2-Dipalmitoylphosphatidylcholine; D005047:Etoposide