Localization of carbohydrates in thick and ultrathin LR white-embedded tissue sections oxidized by acetic anhydride in dimethyl sulfoxide.
The applicability of acetic anhydride (AA) in dimethyl sulfoxide (DMSO) for the oxidation of polysaccharide and their subsequent visualization with thiocarbohydrazide (TCH) and silver proteinate (SP) was evaluated on LR White-embedded thick and ultrathin liver sections. The results of these studies indicated that AA-DMSO-TCH-SP reaction is chemically specific on LR White-embedded tissues and that it offers distinct advantages for the localization of minute glycogen aggregates.
Lo
H K
HK
Department of Surgery, University of Miami School of Medicine, FL 33101.
Malinin
G I
GI
D000085:Acetates / Q000378:metabolism*; D000089:Acetic Anhydrides / Q000378:metabolism*; D000180:Acrylic Resins; D000818:Animals; D050260:Carbohydrate Metabolism; D004121:Dimethyl Sulfoxide; D006003:Glycogen; D006651:Histocytochemistry / Q000379:methods; D008099:Liver / Q000378:metabolism / Q000648:ultrastructure*; D010084:Oxidation-Reduction; D051381:Rats
D000085:Acetates; D000089:Acetic Anhydrides; D000180:Acrylic Resins; C031800:acetic anhydride; D006003:Glycogen; C048707:LR white; D004121:Dimethyl Sulfoxide