A hemolytic assay for the measurement of equine complement.
A hemolytic assay was developed for the measurement of functional equine complement activity. The assay utilizes antibody sensitized chicken erythrocytes as the target cell and was specific for classical pathway (antibody dependent) complement activity. The assay was found to be reproducible and more sensitive than previous reports using other species of target cells. Total serum complement (CH50) values were determined for five mares and their foals and followed over a period of 3 months.
Reis
K J
KJ
Department of Large Animal Clinical Sciences, University of Florida, Gainesville 32610.
D000818:Animals; D000831:Animals, Newborn; D002645:Chickens; D015941:Complement Hemolytic Activity Assay / Q000379:methods*; D003165:Complement System Proteins / Q000032:analysis*; D004912:Erythrocytes; D005260:Female; D006736:Horses / Q000276:immunology*; D007700:Kinetics; D013696:Temperature
D003165:Complement System Proteins