title: Enhancement of in vitro infectivity of simian malaria sporozoites to hepatocytes by centrifugation.
abstract: Sporozoites of Plasmodium cynomolgi, Plasmodium knowlesi, and Plasmodium coatneyi were deposited onto monolayers of hepatocytes from rhesus monkeys (Macaca mulatta). When sporozoites were centrifuged (1,600 g for 5 min), 4-13-fold more schizonts were observed than were found in noncentrifuged control cultures. Centrifugation of hepatocyte monolayers before adding sporozoites did not modify the number of parasites.
authors.affiliation: Malaria Branch, Centers for Disease Control, Atlanta, Georgia 30333.
authors.forename: P
authors.initials: P
authors.lastname: Millet
authors.forename: W E
authors.initials: WE
authors.lastname: Collins
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002498:Centrifugation; D008099:Liver / Q000166:cytology / Q000469:parasitology*; D008253:Macaca mulatta; D010961:Plasmodium / Q000254:growth & development*