Transcription of dispersed repeated sequences during Pleurodeles waltl oogenesis.
Several repeated DNA sequences were isolated from a partial genomic DNA library of the newt Pleurodeles waltl. These repeated DNA elements are dispersed over the 12 P. waltl bivalents, and some of them are transcribed in the oocyte. We describe the localization of label following in situ hybridization to nascent RNA attached to the lateral loops of lampbrush chromosomes. Variations in the number and the location of labelled loops were constantly found for several of the probes. The results are discussed in view of the "cotranscription model" of RNA synthesis on lampbrush chromosomes. We speculate on the possible origins of variation in transcription on lampbrush loops.
Randsholt
N B
NB
Centre de Génétique Moléculaire du CNRS, Université Pierre et Marie Curie, Gif-sur-Yvette, France.
Muller
J P
JP
Loones
M T
MT
D000818:Animals; D001345:Autoradiography; D002875:Chromosomes / Q000502:physiology; D015342:DNA Probes; D005260:Female; D009693:Nucleic Acid Hybridization; D009865:Oocytes / Q000502:physiology / Q000648:ultrastructure; D009866:Oogenesis / Q000235:genetics*; D010999:Pleurodeles / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D015183:Restriction Mapping; D012455:Salamandridae / Q000235:genetics*; D014158:Transcription, Genetic
D015342:DNA Probes