title: Desorption of ions from rat membranes: selectivity of different ionization techniques.
abstract: Complex lipid biomarkers, including phosphatidylcholines, cerebrosides and sulfatides, are shown to be desorbed intact from rat brain myelin and rat liver microsomes by liquid secondary ion mass spectrometry, by plasma desorption and by laser desorption. Different polar lipids are favored by the different desorption techniques and as negative or positive ions. These selectivities support current theories about ionization for the different techniques.
authors.affiliation: Department of Pharamcology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
authors.forename: C
authors.initials: C
authors.lastname: Fenselau
authors.forename: D N
authors.initials: DN
authors.lastname: Heller
authors.forename: J K
authors.initials: JK
authors.lastname: Olthoff
authors.forename: R J
authors.initials: RJ
authors.lastname: Cotter
authors.forename: Y
authors.initials: Y
authors.lastname: Kishimoto
authors.forename: O M
authors.initials: OM
authors.lastname: Uy
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D066298:In Vitro Techniques; D007477:Ions; D008055:Lipids / Q000032:analysis; D008297:Male; D013058:Mass Spectrometry; D008566:Membranes / Q000032:analysis*; D008862:Microsomes, Liver / Q000032:analysis; D008970:Molecular Weight; D009186:Myelin Sheath / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D007477:Ions; D008055:Lipids