title: Arachidonic acid in uterine phospholipid during early pregnancy and following hormone treatment.
abstract: Evidence that prostaglandins are involved in intercellular communication during blastocyst implantation suggested that development and loss of uterine sensitivity to deciduogenic stimuli during early pregnancy might depend upon changes in uterine capacity to mobilize arachidonic acid from phospholipid. We measured levels of arachidonic acid in lipid fractions on Day 6 of pregnancy in uterine segments containing implantation sites, in uterine segments between implantation sites, and in luminal epithelial cells after a deciduogenic stimulus. Arachidonic acid in uterine phospholipid was depleted at implantation sites. With an intrauterine deciduogenic stimulus of hormonally primed ovariectomized rat uteri, the arachidonic acid content of the luminal epithelium decreased. When the fatty acid composition of the luminal epithelium was examined during pseudopregnancy and after progestin-estrogen treatment, however, no changes in arachidonic acid composition and content were observed. These data suggest that during blastocyst implantation, luminal epithelial cells at implantation sites mobilize arachidonic acid from phospholipid for prostaglandin synthesis, but that uterine sensitivity and the capacity to synthesize prostaglandins in response to the blastocyst does not depend upon changes in arachidonic acid levels in uterine phospholipid.
authors.affiliation: Department of Obstetrics and Gynecology, University of Cincinnati College of Medicine, Ohio 45267-0526.
authors.forename: B C
authors.initials: BC
authors.lastname: Moulton
authors.forename: P T
authors.initials: PT
authors.lastname: Russell
mesh_terms: D000818:Animals; D001095:Arachidonic Acids / Q000032:analysis / Q000378:metabolism* / Q000502:physiology; D010064:Embryo Implantation / Q000187:drug effects / Q000502:physiology; D004958:Estradiol / Q000494:pharmacology / Q000502:physiology; D005260:Female; D010743:Phospholipids / Q000032:analysis / Q000378:metabolism*; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology / Q000502:physiology; D011453:Prostaglandins / Q000378:metabolism / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D014599:Uterus / Q000032:analysis / Q000378:metabolism*
chemical_list: D001095:Arachidonic Acids; D010743:Phospholipids; D011453:Prostaglandins; D011374:Progesterone; D004958:Estradiol