[Isolation and various physico-chemical properties of diabetogenic factor from the blood of patients with diabetes].
A preparation of diabetogenic factor was purified 660-fold using affinity chromatography on heparin-Sepharose. Homogeneity of the preparation and its molecular mass (about 60 kDa) were evaluated in, polyacrylamide gel electrophoresis. The fraction with high affinity to heparin exhibited the highest biological activity and was eluted by a solution with high ionic strength (1.5 M NaCl). Biological activity of the diabetogenic factor preparation correlated with its concentration in blood plasma in vitro.
Kudriashov
B A
BA
Liapina
L A
LA
Azieva
L D
LD
D002846:Chromatography, Affinity; D003920:Diabetes Mellitus / Q000097:blood*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood; D005342:Fibrinolysis; D006801:Humans; D008970:Molecular Weight; D010907:Pituitary Hormones / Q000097:blood* / Q000302:isolation & purification
D010907:Pituitary Hormones; C010912:diabetogenic factor, human
Poluchenie i izuchenie nekotorykh fiziko-khimicheskikh svoĭstv diabetogennogo faktora krovi bol'nykh diabetom.