An improved high-performance liquid chromatographic assay for the determination of free and esterified carnitine in animal tissues.
A high-performance liquid chromatographic procedure for the determination of carnitine that is converted to coenzyme A(CoA) stoichiometrically by carnitine acetyltransferase is described. The chromatographic separation was accomplished by using Unisil QC8 column and a mixture of 190 mM KH2PO4 and methanol (87:13, v/v) as eluent. The present method is rapid, sensitive, and can be used for the routine analysis of animal tissues and plasma.
Arakawa
N
N
Department of Food and Nutrition, Ochanomizu University, Tokyo, Japan.
Ha
T Y
TY
Otsuka
M
M
D000123:Acetyltransferases / Q000378:metabolism*; D000818:Animals; D002331:Carnitine / Q000097:blood / Q000378:metabolism*; D002332:Carnitine O-Acetyltransferase / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003065:Coenzyme A / Q000378:metabolism*; D004951:Esterification; D006168:Guinea Pigs; D007668:Kidney / Q000201:enzymology* / Q000378:metabolism; D008099:Liver / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000201:enzymology* / Q000378:metabolism
D000123:Acetyltransferases; D002332:Carnitine O-Acetyltransferase; D002331:Carnitine; D003065:Coenzyme A