title: A preliminary study of brain gliomas with H17E2 monoclonal antibody: immunoscintigraphy and pharmacokinetics.
abstract: Ten patients with relapsing high grade brain gliomas and one patient with low grade glioma were studied with a monoclonal antibody (H17E2) against placental alkaline phosphatase. In addition 2 patients with relapsing high grade glioma were studied with a non specific antibody (4D513/2118). 1 mCi of Iodine-131-labelled H17E2 was administered intracarotidly (i.c.) in two, and intravenously (i.v.) in 9 patients. Immunoscintigrams were taken at 0, 2, 24, 48 and 72 hours. Radioactivity was monitored in blood and urine. Tumour/non-tumour ratios were estimated (max. 2.45). All high grade gliomas receiving specific antibody irrespective of the route of administration, gave a positive immunoscintigraphic pattern, increasing in intensity with time. Disappearance of radioactivity in blood was biexpontential with a long component over 30 hours. Urinary excretion of radioactivity ranged from 3.7-21.7% of administered dose/day. The patient with low grade glioma and the patients receiving non specific monoclonal antibody showed a negative pattern, a fast blood clearance and a high urinary excretion. We conclude that a) Iodine-131 labelled H17E2 proved to be stable in vivo and produced satisfactory tumour localisation and b) i.v. route was as good as i.c.
authors.affiliation: Agii Anargiri Cancer Hospital, Athens, Greece.
authors.forename: D V
authors.initials: DV
authors.lastname: Skarlos
authors.forename: J
authors.initials: J
authors.lastname: Malamitsi
authors.forename: G B
authors.initials: GB
authors.lastname: Sivolapenko
authors.forename: N
authors.initials: N
authors.lastname: Demakopoulos
authors.forename: G
authors.initials: G
authors.lastname: Avgoustatos
authors.forename: C
authors.initials: C
authors.lastname: Koutoulidis
authors.forename: A A
authors.initials: AA
authors.lastname: Epenetos
mesh_terms: D000469:Alkaline Phosphatase / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000008:administration & dosage; D014408:Biomarkers, Tumor / Q000276:immunology; D001932:Brain Neoplasms / Q000175:diagnosis* / Q000000981:diagnostic imaging / Q000276:immunology; D005910:Glioma / Q000175:diagnosis* / Q000000981:diagnostic imaging / Q000276:immunology; D006801:Humans; D007457:Iodine Radioisotopes; D007700:Kinetics; D011877:Radionuclide Imaging
chemical_list: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D007457:Iodine Radioisotopes; D000469:Alkaline Phosphatase