Demonstration of neutrophil chemotactic anaphylatoxins in human dandruff.
In contrast to scales collected from the scalps of nine healthy individuals where a few parakeratotic cells are observable, a large number of parakeratotic cells associated with some infiltrated polymorphonuclear leukocytes (PMNLs) were found in the scales obtained from 11 individuals complaining of dandruff. Therefore, we determined the neutrophil chemotactic properties of the water-soluble extracts of dandruff scales and normal control scalp scales. Aqeous extracts fractionated by Sephadex G-75 showed a potent chemotactic activity only in the fractions of the dandruff patients that eluted with cytochrome C marker (cyt C; molecular weight, 12 kDa). It was comparatively stable to heat but was greatly inhibited by the addition of anti-C5 antiserum. Radioimmunoassay demonstrated that, although small amounts of C5a and C4a anaphylatoxins were demonstratable even in the extracts of normal scalp, they were found in significantly increased amounts in the extracts of dandruff. Moreover, there was a significantly positive correlation between C5a and C4a concentrations in these extracts. These results suggest that classical complement pathway activation with resultant production of C5a anaphylatoxin is involved in the migration of PMNLs into the lesional skin of dandruff.
Kikuchi
T
T
Shiseido Basic Research Laboratories, Yokohama, Japan.
Horii
I
I
Sakamoto
T
T
Nakayama
Y
Y
Tagami
H
H
D000328:Adult; D000706:Anaphylatoxins / Q000378:metabolism*; D002630:Chemotactic Factors / Q000378:metabolism*; D002634:Chemotaxis, Leukocyte; D002850:Chromatography, Gel; D015934:Complement C4a / Q000378:metabolism; D003182:Complement C5 / Q000378:metabolism*; D015936:Complement C5a / Q000378:metabolism; D006801:Humans; D008297:Male; D009504:Neutrophils / Q000166:cytology*; D011863:Radioimmunoassay; D012536:Scalp Dermatoses / Q000378:metabolism* / Q000473:pathology
D000706:Anaphylatoxins; D002630:Chemotactic Factors; D003182:Complement C5; D015934:Complement C4a; D015936:Complement C5a