Stimulation of prostaglandin synthesis by cholecystokinin in primary culture cells of bovine gallbladder muscle.
Primary culture cells derived from bovine gallbladder muscles synthesize PGE2 as a major cyclooxygenase product with a trace amount of 6-keto-PGF1 alpha-like material. The synthesis of PGE2 and total cyclooxygenase products was enhanced in response to cholecystokinin (CCK). In the presence of indomethacin the synthesis of PG was inhibited and the release of arachidonic acid (AA) in response to CCK was enhanced. These data suggest that CCK may stimulate the release of AA, probably by activating phospholipase A2/C, from membrane phospholipids in the gallbladder muscle.
Hidaka
T
T
Department of Medical Biochemistry, Kurume University School of Medicine, Fukuoka, Japan.
Nakano
M
M
Shingu
M
M
Sugiyama
M
M
Inokuchi
T
T
Ogura
R
R
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D002766:Cholecystokinin / Q000494:pharmacology*; D005704:Gallbladder / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009132:Muscles / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D011453:Prostaglandins / Q000096:biosynthesis* / Q000493:pharmacokinetics
D011453:Prostaglandins; D002766:Cholecystokinin