Investigations on the deformability of human red blood cells stored in different preservative solutions: relation between cellular hemoglobin concentration and in vivo survival.
In continuation of earlier studies (Kucera, W. et al.: Biomed. Biochim. Acta 44, 1459-1467, (1985); Wegner, G. et al.: Biomed. Biochim. Acta 46, 605-609 (1987] the in vivo of human red blood cells (RBC) stored for 35 or 42 days in different anticoagulant and preservative media was analyzed with regard to the main influencing factors. We found a significant partial correlation (2 alpha = 0.001; n = 75) between the in vivo survival and the mean cellular hemoglobin concentration (MCHC; rp = -0.70) as well as on a lower level (2 alpha = 0.01; n = 75) with the ATP content (rp = 0.35). By calculating the internal viscosity as a determinant of RBC deformability from MCHC values and non-linear fitting an equation for the survival prediction for known MCHC values of stored RBC was found. Low pressure filterability through cellulose filters characterizing cellular deformability is interpreted in relation to viability of stored RBC.
Wegner
G
G
Institut für Transfusiologie und Transplantologie, Humboldt-Universität, Berlin, DDR.
Kucera
W
W
D001793:Blood Preservation; D002470:Cell Survival; D004907:Erythrocyte Deformability; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques
D006454:Hemoglobins