[Assessment of cardiac rejection following heart and lung transplantation (I)].
To diagnose the cardiac rejection after the heart and lung transplantation, the histopathological assessment is the most reliable method at the present time. In this study, the heterotopic heart and lung transplantation was performed using rat model and the rejection process of heart allograft was sequentially examined at 1 day, 4 days, 7 days, and 12 days after the transplantation. The gross finding of both ventricles were observed to investigate the development process of the rejection. Then, the most typical findings in each period were analysed using a microscope with our criteria including three components, which are 1. Perivascular findings 2. Interstitial findings, 3. Myocyte degeneration. This study demonstrates that allograft rejection after heart and lung transplantation initiates from right ventricular free wall and spreads into outer layer of left ventricle as same fashion as orthotopic cardiac transplantation. The assessment of these 3 components using the indicators shown in this study might be very useful method to objectively determine the grades of rejection after the heart and lung transplantation.
Hachida
M
M
Kurosawa
H
H
Koide
M
M
Koyanagi
H
H
D000818:Animals; D006084:Graft Rejection; D006339:Heart Rate; D016041:Heart-Lung Transplantation / Q000473:pathology*; D008168:Lung / Q000473:pathology*; D008297:Male; D009206:Myocardium / Q000473:pathology*; D051381:Rats; D011915:Rats, Inbred BUF; D011917:Rats, Inbred Lew; D016042:Transplantation, Heterotopic / Q000473:pathology*