Multiple splice forms of ribonuclease-inhibitor mRNA differ in the 5'-untranslated region.
We report the sequence of the cDNAs representing five independent splice forms of human placental RNase inhibitor (RI) mRNA. RI mRNAs differ principally in the 5'-untranslated region, which may include or lack a 68-nucleotide (nt) exon inserted at a splice site located only 20 nt upstream from the initiator AUG. At least three other exons may also abut the same splice site. This unusual and variable feature of the mRNA would suggest that secondary structure in the region of the start codon may differ among RI messages. A single copy of the RI gene exists in the human genome.
Crawford
D
D
Howard Hughes Medical Institute, U.T. Southwestern Medical Center, Dallas 75235-9050.
Hagerty
K
K
Beutler
B
B
D000595:Amino Acid Sequence; D001482:Base Composition; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D015345:Oligonucleotide Probes; D010926:Placental Hormones / Q000235:genetics*; D011247:Pregnancy; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics*; D015183:Restriction Mapping; D012260:Ribonucleases / Q000037:antagonists & inhibitors
D015345:Oligonucleotide Probes; D010926:Placental Hormones; D012333:RNA, Messenger; C022052:placental ribonuclease inhibitor; D012260:Ribonucleases