Simultaneous phenotyping of erythrocyte acid phosphatase and esterase D by nonequilibrium agarose isoelectric focusing.
Nonequilibrium agarose isoelectric focusing in a pH 4.5-7 gradient with 3-(N-morpholinopropanesulfonic acid (MOPS) and taurine as chemical separators presents a fast, easy and reliable method for the simultaneous determination of the common erythrocyte acid phosphatase and esterase D phenotypes in hemolysates and dried bloodstains. The application of the method to experimental and casework bloodstains is described.
Dimo-Simonin
N
N
Institut de Médecine légale, Faculté de Médecine del'Université de Lausanne, Switzerland.
Brandt-Casadevall
C
C
Gujer
H R
HR
D000135:Acid Phosphatase / Q000097:blood* / Q000235:genetics; D000483:Alleles; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000097:blood* / Q000235:genetics; D004586:Electrophoresis; D004587:Electrophoresis, Agar Gel; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods*; D010641:Phenotype
D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; C020118:ESD protein, human; D000135:Acid Phosphatase