title: Analysis of the specificity of human antibodies to antigens of Leishmania braziliensis braziliensis.
abstract: The antigenicity of promastigotes of Leishmania braziliensis braziliensis (L. b. braziliensis) treated with 1% sodium desoxycholate in 10 mM Tris-Hcl pH 8.2 was analysed by immunoblot using as probes sera from American cutaneous leishmaniasis (ACL), American visceral leishmaniasis (AVL), schistosomiasis, malaria and Chagas' disease. The ACL sera reacted constantly with a 60 kD band. No reactivity to this protein was observed with sera from the other diseases above mentioned indicating that the 60 kD protein may be used in serodiagnosis for ACL.
authors.forename: A
authors.initials: A
authors.lastname: Masuda
authors.forename: S F
authors.initials: SF
authors.lastname: do Nascimento
authors.forename: C S
authors.initials: CS
authors.lastname: Guerra
authors.forename: G da S
authors.initials: Gda S
authors.lastname: Paranhos
authors.forename: A W
authors.initials: AW
authors.lastname: Ferreira
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis*; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D000953:Antigens, Protozoan / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting; D007891:Leishmania / Q000276:immunology*; D007892:Leishmania braziliensis / Q000276:immunology*; D007896:Leishmaniasis / Q000276:immunology; D007897:Leishmaniasis, Mucocutaneous / Q000276:immunology; D007898:Leishmaniasis, Visceral / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan