title: Effects of ethanol in vitro on some parameters of the immune response.
abstract: The action of ethanol (ETH) on murine lymphocyte subpopulations and on human peripheral blood mononuclear cells (PBMC) stimulated in vitro by mitogens was studied. ETH caused a concentration-dependent decrease in DNA synthesis in the different murine cell types. ETH was more immunosuppressive for T lymphocytes than for B lymphocytes. An enhancement of the blastogenic response was observed for B cells at 0.5% ETH. Interleukin 2 synthesis by murine splenocytes was inhibited by ETH in a concentration-related manner; the lowest concentrations of ETH caused an increase in interleukin 2 synthesis. The highest concentrations of ETH tested decreased the size of the cell clusters formed in cultures of mitogen-stimulated lymphocytes, whereas an increase in PBMC cluster size was observed in the presence of 0.5 and 1% ETH.
authors.affiliation: Laboratoire de Biochimie des Membranes, ENSCM, Montpellier, France.
authors.forename: C
authors.initials: C
authors.lastname: Levallois
authors.forename: N
authors.initials: N
authors.lastname: Rouahi
authors.forename: J L
authors.initials: JL
authors.lastname: Balmes
authors.forename: J C
authors.initials: JC
authors.lastname: Mani
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D000431:Ethanol / Q000494:pharmacology*; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D013154:Spleen / Q000166:cytology; D013950:Thymus Gland / Q000166:cytology
chemical_list: D007376:Interleukin-2; D000431:Ethanol; D004247:DNA