[The excessive activity of cytochrome P450 system in ALS--the metabolic ratio of sparteine].
The activity of cytochrome P450 family was studied in patients with amyotrophic lateral sclerosis (ALS) in an attempt to evaluate the susceptibility to environmental substances including possible some neurotoxin. The metabolic ratio (MR) of sparteine sulfate was measured in 30 patients with ALS and 41 controls for the evaluation of the sparteine nitro (N)-oxidation with cytochrome P450 system. The population of phenotypes designed as extensive metabolisers (EMs), intermediate metabolisers (IMs), and poor metabolisers (PMs) for sparteine nitro (N)-oxidation was determined in each group. The lower MR (p less than 0.02) and higher frequency of EMs (p less than 0.05) were observed in ALS group, suggesting the excessive activity of N-oxidation with cytochrome P450 pathway in the ALS group. Especially in 15 patients with ALS age before 60, these tendencies were more remarkable with a significant lower MR (p less than 0.01) and extremely high frequency of EMs (p less than 0.005). The present study might suggest the possible participation of cytochrome P450 pathway in the development of ALS.
Kaneko
K
K
Atsumi
T
T
Miyataka
T
T
D000328:Adult; D000368:Aged; D000690:Amyotrophic Lateral Sclerosis / Q000201:enzymology / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009584:Nitrogen / Q000378:metabolism; D010084:Oxidation-Reduction; D013034:Sparteine / Q000378:metabolism*
D013034:Sparteine; D003577:Cytochrome P-450 Enzyme System; D009584:Nitrogen