Influence of oxidised dietary oil and antioxidant supplementation on membrane-bound lipid stability in broiler meat.
1. The effects of oxidised oil, dietary alpha-tocopherol and BHA/BHT-supplementation on the fatty acid composition of mitochondrial, microsomal and soluble protein fractions of broiler muscles, and on their lability to metmyoglobin/hydrogen peroxide-catalysed peroxidation were investigated. 2. Oxidised oil in the broiler diets induced rapid oxidation of the membrane-bound lipids and decreased their stability towards metmyoglobin-hydrogen peroxide-catalysed peroxidation. 3. Supplementation of the broiler diets with alpha-tocopherol increased the alpha-tocopherol concentrations in the microsomal and soluble protein fractions of the dark meat as well as the soluble protein fraction of the white meat. This, in turn, stabilised the membrane-bound lipids against metmyoglobin/hydrogen peroxide-initiated peroxidative changes.
Asghar
A
A
Department of Food Science and Human Nutrition, Michigan State University, East Lansing 48824.
Lin
C F
CF
Gray
J I
JI
Buckley
D J
DJ
Booren
A M
AM
Crackel
R L
RL
Flegal
C J
CJ
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002083:Butylated Hydroxyanisole / Q000494:pharmacology*; D002084:Butylated Hydroxytoluene / Q000494:pharmacology*; D002645:Chickens / Q000378:metabolism*; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology*; D005227:Fatty Acids / Q000032:analysis; D015227:Lipid Peroxidation / Q000187:drug effects*; D008460:Meat / Q000032:analysis / Q000592:standards*; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism*; D008931:Mitochondria, Muscle / Q000187:drug effects / Q000378:metabolism*; D010743:Phospholipids / Q000032:analysis / Q000378:metabolism*; D010938:Plant Oils / Q000494:pharmacology; D000074242:Sunflower Oil; D014810:Vitamin E / Q000494:pharmacology*
D000975:Antioxidants; D004042:Dietary Fats, Unsaturated; D005227:Fatty Acids; D010743:Phospholipids; D010938:Plant Oils; D000074242:Sunflower Oil; D014810:Vitamin E; D002084:Butylated Hydroxytoluene; D002083:Butylated Hydroxyanisole