title: [Methodologic studies of the isolation of VLDL using the lipoprotein precipitation reaction in the preparation of apolipoprotein E (Apo E)].
abstract: Several lipoprotein precipitation reactions were examined in the isolation of VLDL for apo E phenotyping in comparison to ultracentrifugation. MgCl2-Heparin- and phosphotungstic acid precipitation revealed the best results, although an apo E phenotyping was possible in only 40% of the samples. Ultracentrifugation was unequivocally superior to lipoprotein precipitation reactions.
authors.forename: B
authors.initials: B
authors.lastname: Gromoll
authors.forename: S
authors.initials: S
authors.lastname: Krantz
mesh_terms: D001057:Apolipoproteins E / Q000032:analysis*; D011232:Chemical Precipitation; D006801:Humans; D008079:Lipoproteins, VLDL / Q000302:isolation & purification*; D014461:Ultracentrifugation
chemical_list: D001057:Apolipoproteins E; D008079:Lipoproteins, VLDL
vernacular_title: Methodische Untersuchungen zur Isolierung von VLDL mit Hilfe von Lipoprotein-Fällungsreaktionen bei der Darstellung von Apolipoprotein E (Apo E).