Transient production and secretion of human transforming growth factor TGF-beta 2.
Transient transfection of simian COS cells with a recombinant plasmid encoding the human transforming growth factor TGF-beta 2 precursor protein results in the production of a latent, biologically inactive protein. Upon acidification, recombinant TGF-beta 2 exhibits full biological activity, including inhibition of mink lung epithelial cell growth, stimulation of anchorage-independent growth of murine embryonic fibroblasts, and competition for TGF-beta receptor binding. Further analysis of conditioned media with antiserum to either a pro- [amino acid (aa) residues 1-220] or mature [aa 297-414] peptide of the TGF-beta 2 precursor suggests that TGF-beta 2, similar to TGF-beta 1 production in Chinese hamster ovary cells [Gentry et al., Mol. Cell. Biol. 7 (1987) 3418-3427], is initially synthesized as a larger precursor protein which is proteolytically cleaved to yield the mature 112-aa transforming growth factor.
Caltabiano
M M
MM
Department of Cell Sciences, Smith Kline and French Laboratories, King of Prussia, PA 19406.
Tsang
M L
ML
Weatherbee
J A
JA
Lucas
R
R
Sathe
G
G
Sutton
J
J
Johnson
G D
GD
Bergsma
D J
DJ
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D005822:Genetic Vectors; D006801:Humans; D007106:Immune Sera; D015151:Immunoblotting; D008970:Molecular Weight; D010957:Plasmids; D011498:Protein Precursors / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis / Q000494:pharmacology; D014162:Transfection; D015291:Transforming Growth Factors / Q000096:biosynthesis / Q000235:genetics*
D007106:Immune Sera; D011498:Protein Precursors; D011994:Recombinant Proteins; D015291:Transforming Growth Factors