title: Regulation of cytosolic Ca2+ in resting and stimulated rat submandibular salivary gland acini.
abstract: To determine the range over which cytosolic Ca2+ concentration (Cai2+) is regulated, Cai2+ was measured in salivary acini loaded with Fura-2. With extracellular Ca2+ (Cao2+) = 2.5 mM, mean resting Cai2+ (+/- SD) was 103 +/- 20 nM (n = 29). Carbachol increased Cai2+ immediately to 288 +/- 81 nM, which then declined to a sustained level of 253 +/- 58 nM. When Cao2+ was less than 10 nM, the immediate response to carbachol was little affected but the sustained response was abolished. Atropine abolished all responses to carbachol. Changes in Cai2+ were not due to Ca2+-induced Ca2+ release or to Ca2+ influx via voltage-dependent channels. With Cao2+ = 2.5 mM, epinephrine raised Cai2+ initially to 196 +/- 57 nM (n = 6), which soon declined to stable levels of 178 +/- 40 nM. When Cao2+ was less than 10 nM the prolonged response to epinephrine was abolished but the initial increase was relatively unaffected. All epinephrine-induced changes in Cai2+ were abolished by prazosin. The range of Cai2+ measured corresponds to that over which non-mitochondrial Ca2+ transport is stimulated in permeabilized submandibular gland acini suggesting that such a Ca2+ transport system may regulate physiological changes in Cai2+ in these acini in the rat.
authors.affiliation: Department of Child Health, University of Missouri School of Medicine, Columbia 65212.
authors.forename: R W
authors.initials: RW
authors.lastname: Brinck
authors.forename: T W
authors.initials: TW
authors.lastname: Hurley
mesh_terms: D000818:Animals; D001285:Atropine / Q000494:pharmacology; D001572:Benzofurans; D002118:Calcium / Q000032:analysis / Q000493:pharmacokinetics*; D002217:Carbachol / Q000494:pharmacology; D002614:Chelating Agents; D003600:Cytosol / Q000032:analysis / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004837:Epinephrine / Q000494:pharmacology; D005110:Extracellular Space / Q000032:analysis / Q000378:metabolism; D005456:Fluorescent Dyes; D016257:Fura-2; D015759:Ionomycin / Q000494:pharmacology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013363:Submandibular Gland / Q000166:cytology* / Q000378:metabolism
chemical_list: D001572:Benzofurans; D002614:Chelating Agents; D005456:Fluorescent Dyes; D015759:Ionomycin; D001285:Atropine; D002217:Carbachol; D002118:Calcium; D016257:Fura-2; D004837:Epinephrine