title: Steric course of the hydrolysis of alpha,alpha-trehalose and alpha-D-glucosyl fluoride catalyzed by pig kidney trehalase.
abstract: We are unable to confirm the report of Labat et al.3 that pig kidney trehalase hydrolyzes alpha,alpha-trehalose to form solely alpha-D-glucose. Highly purified trehalase from pig renal cortex was found, in reactions monitored by 1H-n.m.r. spectra, to hydrolyze alpha,alpha-trehalose with the formation of both alpha- and beta-D-glucose. That the beta anomer constitutes the enzymically mobilized glucosyl residue is indicated by the further finding that beta-D-glucose is the product formed on hydrolysis of alpha-D-glucosyl fluoride by the enzyme. Present results show the stereochemical behavior of pig kidney trehalase in hydrolyzing alpha,alpha-trehalose to be indistinguishable from that reported by ourselves and others for trehalase preparations from a range of biological sources including rabbit renal cortex.
authors.affiliation: Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461.
authors.forename: M
authors.initials: M
authors.lastname: Nakano
authors.forename: C F
authors.initials: CF
authors.lastname: Brewer
authors.forename: T
authors.initials: T
authors.lastname: Kasumi
authors.forename: E J
authors.initials: EJ
authors.lastname: Hehre
mesh_terms: D000818:Animals; D004187:Disaccharides; D005947:Glucose / Q000031:analogs & derivatives*; D006868:Hydrolysis; D007668:Kidney / Q000201:enzymology*; D009682:Magnetic Resonance Spectroscopy; D013237:Stereoisomerism; D013552:Swine; D014198:Trehalase / Q000378:metabolism*; D014199:Trehalose
chemical_list: D004187:Disaccharides; C030364:glucosyl fluoride; D014199:Trehalose; D014198:Trehalase; D005947:Glucose