Oxalate, phosphate and sulphate determination in serum and urine by ion chromatography.
A rapid method for the determination of phosphate, sulphate and oxalate in serum by ion chromatography is described. Serum is deproteinized through a Centrifree filter by centrifugation and the ultrafiltrate directly injected into an ion chromatograph equipped with an anion exchange column and a conductivity detector. By this procedure the sample is not diluted and even small amounts of oxalate in biological fluids can be detected. Mean serum concentrations found in healthy individuals are: phosphate 1.07 mmol/l; sulphate 0.35 mmol/l; oxalate 21.02 mumols/l. Phosphate, sulphate and oxalate contents were also determined in urine from healthy individuals. Values found in serum and urine are in good agreement with those previously reported.
Politi
L
L
Dipartimento di Scienze e Tecnologie Biochimiche e Biometria, Università dell'Aquila, Rome, Italy.
Chiaraluce
R
R
Consalvi
V
V
Cerulli
N
N
Scandurra
R
R
D002852:Chromatography, Ion Exchange / Q000379:methods*; D006801:Humans; D010070:Oxalates / Q000032:analysis* / Q000097:blood / Q000652:urine; D010710:Phosphates / Q000032:analysis* / Q000097:blood / Q000652:urine; D012015:Reference Standards; D013431:Sulfates / Q000032:analysis* / Q000097:blood / Q000652:urine
D010070:Oxalates; D010710:Phosphates; D013431:Sulfates