Galactosyl residue in exopolysaccharide from Rhizobium meliloti JJ-1 exposed to manganese in furanoid.
Exopolysaccharide (EPS) elaborated by Rhizobium meliloti JJ-1 in a manganese supplemented medium was isolated. Periodate oxidation, reduction with sodium borohydride, followed by hydrolysis and subsequent capillary gas liquid chromatography of the derived alditol acetates revealed that D-galactose in this complex biopolymer is in furanoid form. This observation was further confirmed by 13carbon nuclear magnetic resonance (13C NMR).
Appanna
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Chemistry Department, McGill University, Montreal, Canada.
D002851:Chromatography, High Pressure Liquid; D005109:Extracellular Matrix / Q000032:analysis*; D005663:Furans / Q000032:analysis*; D005690:Galactose / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D008345:Manganese; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification*; D012231:Rhizobium / Q000032:analysis* / Q000254:growth & development
D005663:Furans; D011135:Polysaccharides, Bacterial; D008345:Manganese; D005690:Galactose