A lignin peroxidase-encoding cDNA from the white-rot fungus Phlebia radiata: characterization and expression in Trichoderma reesei.
The nucleotide sequence of a cDNA coding for a lignin peroxidase (Lgp) of the white-rot fungus, Phlebia radiata, has been determined. By amino acid (aa) sequencing, it has been shown that the protein product of this gene is the LIII Lgp of Pb. radiata. The isolated gene and the putative aa sequence are about 60% homologous to published Lgp sequences from the fungus, Phanerochaete chrysosporium. The aa thought to be involved in the catalysis of LIII are revealed by comparison with the yeast cytochrome c peroxidase. The P. radiata Lgp-encoding gene (lgp3) was expressed in the fungus, Trichoderma reesei, under the cellobiohydrolase-encoding cbh1 gene promoter. Lgp3 mRNA was produced by the T. reesei transformants. No Lgp protein, however, could be detected.
Saloheimo
M
M
VTT, Biotechnical Laboratory, Espoo, Finland.
Barajas
V
V
Niku-Paavola
M L
ML
Knowles
J K
JK
D000363:Agaricales / Q000201:enzymology / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D004271:DNA, Fungal / Q000235:genetics*; D015723:Gene Library; D005800:Genes, Fungal; D003904:Mitosporic Fungi / Q000235:genetics*; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000235:genetics*; D010957:Plasmids; D011487:Protein Conformation; D012689:Sequence Homology, Nucleic Acid; D014242:Trichoderma / Q000201:enzymology / Q000235:genetics*
D004271:DNA, Fungal; D010544:Peroxidases; C042858:lignin peroxidase