[ELISA method for the determination of factor VII antigen].
The low plasma concentration of clotting factor VII makes it difficult to assay its antigenic fraction by the conventional methods of precipitation with specific antigens. Simple and peroxidase-conjugated antisera are currently available from commercial sources, thus allowing one to determine F VII:Ag by enzyme immunoassay. An ELISA method has been developed in this laboratory which provides sensitivity limits about 0.1% of the plasma concentration of F VII and correlates significantly with its functional activity (r = 0.603, n = 44, p less than 0.001). This technique can be highly helpful in characterising molecular variants of F VII, as well as in detecting acquired deficiencies of this factor.
Jorquera
J I
JI
Aznar
J A
JA
Monteagudo
J
J
Montoro
J M
JM
Casaña
P
P
Pascual
I
I
Bañuls
E
E
Curats
R
R
Llopis
F
F
D000941:Antigens / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005167:Factor VII / Q000032:analysis / Q000276:immunology*; D006801:Humans
D000941:Antigens; C042580:factor VII clotting antigen; D005167:Factor VII
Método ELISA para la determinación del antígeno del factor VII.