Flow cytometric methods for the analysis of human basophil surface antigens and viability.
Fluorescence and flow microfluorometric methods have been established for the detection and evaluation of IgE-bearing human leukocytes in various cell preparations including those where basophils are present at low percentages. Quantitative techniques for the determination of basophil purity, viability, and cell surface antigens including IgE are described. Use of these methods will facilitate the identification and phenotypic analysis of human IgE-bearing cells in a wide variety of biological fluids.
Bochner
B S
BS
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21239.
McKelvey
A A
AA
Schleimer
R P
RP
Hildreth
J E
JE
MacGlashan
D W
DW
D015703:Antigens, CD / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D001491:Basophils / Q000276:immunology*; D002469:Cell Separation / Q000379:methods; D002470:Cell Survival; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis*
D015703:Antigens, CD; D000954:Antigens, Surface; D006684:HLA-DR Antigens; D007073:Immunoglobulin E