Muramyl-peptide/gastrin conjugates as potential immunogens.
For a selective covalent linkage of muramyl-peptides with human-little-gastrin the maleimide-thiol reaction principle was adopted. For this purpose thiol-functionalized muramyl-peptide derivatives, i.e. N-acetyl-muramyl-alanyl-D-isoglutaminyl-cysteamine and N-acetyl-muramyl-alanyl-D-isoglutaminyl-N epsilon-palmitoyl-lysyl-cysteamine, were reacted with N alpha-maleoyl-beta-alanyl-[15-methoxinine]-human-little-g ast rin-I-[2-17]. The resulting gastrin conjugates were used in immunization experiments on rabbits and mice. Unexpectedly, these gastrin derivatives proved to be poorly immunogenic despite the built-in immunoadjuvanticity. The titers of the antipeptide antibodies as well as of the unspecific immunoglobulins were in the range of those of the control group.
Moroder
L
L
Max-Planck-Institut für Biochemie, Abteilung Peptidchemie, Martinsried bei München.
Dufresne
M
M
Göhring
W
W
Wünsch
E
E
Leidinger
E
E
Gemeiner
M
M
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000031:analogs & derivatives*; D000276:Adjuvants, Immunologic; D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005755:Gastrins / Q000138:chemical synthesis* / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000032:analysis*; D007114:Immunization; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits
D000276:Adjuvants, Immunologic; D005755:Gastrins; D007106:Immune Sera; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D000119:Acetylmuramyl-Alanyl-Isoglutamine; C013550:gastrin 17