Crystallization and preliminary X-ray study of porcine trypsin, free and complexed with Ecballium elaterium trypsin inhibitor, a member of the squash inhibitors family.
Porcine trypsin has been crystallized either free or complexed with synthetic Ecballium elaterium trypsin inhibitor II, a 28-residue peptide with three disulfide bridges. The crystals diffract beyond 2.0 A. Crystals are orthorhombic, space group P2(1)2(1)2(1), with cell dimensions a = 77.32 A, b = 53.81 A, c = 46.91 A, for the free trypsin, and a = 62.25 A, b = 62.27 A, c = 84.66 A for the complex with E. elaterium trypsin inhibitor II.
Gaboriaud
C
C
Laboratoire de Minéralogie-Cristallographie, CNRS URA09, Université P6, Paris, France.
Vaney
M C
MC
Bachet
B
B
Le-Nguyen
D
D
Castro
B
B
Mornon
J P
JP
D000818:Animals; D003461:Crystallography; D010940:Plant Proteins / Q000648:ultrastructure; D011487:Protein Conformation; D013552:Swine; D014357:Trypsin; D014361:Trypsin Inhibitors / Q000648:ultrastructure*; D014961:X-Ray Diffraction
D010940:Plant Proteins; D014361:Trypsin Inhibitors; D014357:Trypsin