Ultrastructure of a new generation of odontoblasts in grafted coronal tissues of mouse molar tooth germs.
Third molar tooth germs were removed from 14-day-old mice and freed from the enamel organ and follicle. After section of the apical tissues, including Hertwig's sheath, they were transplanted in 1-day-old newborn mice of the same lineage. Electron microscopy of grafts removed 7, 14 and 21 days later showed that, following the disappearance of the initial layer of odontoblasts and a period of adaptation, 14 days after transplantation newly differentiated odontoblasts deposited tubular dentine. The dentine matrix production was increased over that of controls, demonstrating that synthesis was accelerated, possibly because of lack of nerves in the grafts. Numerous characteristic structures that might be involved in the transit of proteoglycans from the Golgi apparatus were seen, as far as the extremity of the odontoblast processes. The particular experimental conditions allowed the observation in the neck region of the odontoblast of a concentration of coated vesicles which might be involved in cellular lengthening. Thus, in the presence of a fine and regular vascular network, a new generation of odontoblasts may differentiate, even in the absence of epithelial and nervous elements, and so predentine may contain inductive factors that allow the odontoblastic differentiation of pulp cells in contact with it.
Héritier
M
M
Laboratoire de Biologie Buccale, Faculté de Chirurgie Dentaire, Lille, France.
Dangleterre
M
M
Bailliez
Y
Y
D000818:Animals; D002454:Cell Differentiation; D003593:Cytoplasm / Q000648:ultrastructure; D003782:Dental Pulp / Q000166:cytology; D003804:Dentin / Q000166:cytology; D005347:Fibroblasts / Q000648:ultrastructure; D006056:Golgi Apparatus / Q000648:ultrastructure; D051379:Mice; D008964:Molar, Third / Q000196:embryology; D009804:Odontoblasts / Q000166:cytology / Q000648:ultrastructure*; D015388:Organelles / Q000648:ultrastructure; D014083:Tooth Germ / Q000166:cytology / Q000637:transplantation*