An adsorption-elution technique for the recovery of influenza virus from water.
A virus adsorption-elution (viradel) procedure was modified and evaluated for the concentration of influenza virus from water. Influent pH, flow rate, eluent pH and composition, and a second-step concentration method were evaluated. The viradel procedure in combination with a chicken erythrocyte adsorption technique resulted in up to 3200-fold concentration of influenza virus from 100 liters of tap water.
Roepke
D C
DC
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Minnesota, St. Paul 55108.
Halvorson
D A
DA
Goyal
S M
SM
Kelleher
C J
CJ
D000327:Adsorption; D000818:Animals; D006863:Hydrogen-Ion Concentration; D009975:Orthomyxoviridae / Q000032:analysis / Q000302:isolation & purification*; D014773:Virology / Q000379:methods*; D014871:Water Microbiology