Binding properties of biotinylated epidermal growth factor to its receptor on cultured cells and tissue sections.
A biotinylated derivative of murine epidermal growth factor (EGF) was prepared by covalent attachment of the terminal amino group of EGF to N-biotinyl-epsilon-aminocaproyl-N-hydroxysuccinimide. The stoichiometry of biotin incorporation was in the range of one biotin moiety per EGF molecule. The biotinylated EGF (biotinyl-epsilon-caproyl-EGF, BioEGF) binds to EGF receptors on intact Ehrlich ascites carcinoma (EAC) cells with an affinity similar to that of native EGF and displays the same mitogenic activity as EGF in a soft agar test system with normal rat kidney (NRK) cells. BioEGF was visualized on cultured cells and tissue sections of a head and neck tumour by commercial streptavidin/avidin detection systems. Cytochemical analyses of certain tumour forms can be easily performed using the BioEGF probe.
Spitzer
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E
Department of Cell Biology, Academy of Sciences of the GDR, Berlin-Buch.
de Los Angeles
M
M
Perez
R
R
Grosse
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R
D000818:Animals; D001665:Binding Sites; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism* / Q000648:ultrastructure; D004815:Epidermal Growth Factor / Q000138:chemical synthesis / Q000378:metabolism*; D066246:ErbB Receptors / Q000378:metabolism*; D006651:Histocytochemistry; D051379:Mice; D008934:Mitogens; D051381:Rats; D014407:Tumor Cells, Cultured / Q000378:metabolism
D008934:Mitogens; C030473:biotinyl epidermal growth factor; D004815:Epidermal Growth Factor; D066246:ErbB Receptors