Immunohistochemical analysis of brain macrophages in adrenoleukodystrophy.
Using a panel of monoclonal antibodies directed against antigenic determinants present at the surface of mononuclear cells, we characterized the macrophage infiltration in frozen brain specimens from a patient with adrenoleukodystrophy by immunohistochemical staining. This study demonstrated that macrophages were the main infiltrating cells in the white matter and that there was a wide variability in their phenotype according to their location: they stained very strongly with Leu-M5, EBM11, Leu-M3, To5 and 2LPM 19 C in the perivascular cuffs, whereas they stained principally with Leu-M5 and EBM11 and to a lesser extent with To5 and 2LPM19c in the white matter. In addition to the expression of MHC class II and CD4 molecules, macrophages exhibited strong labelling with 7G7B6, a monoclonal antibody raised against IL2 receptors. These results attest that macrophages, which infiltrate the brain parenchyma have various phenotypes, and very likely different states of activation, and suggest that interleukin-2 plays an important role during this activation.
Boutin
B
B
Unité de recherche sur les virus, INSERM U43, Hôpital Saint Vincent de Paul, Paris.
Matsuguchi
L
L
Lebon
P
P
Ponsot
G
G
Arthuis
C
C
D000326:Adrenoleukodystrophy / Q000276:immunology* / Q000473:pathology; D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000378:metabolism*; D001921:Brain / Q000276:immunology* / Q000473:pathology; D002648:Child; D002549:Diffuse Cerebral Sclerosis of Schilder / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D008264:Macrophages / Q000276:immunology*; D008297:Male
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation