title: Visualization of interleukin-2-like molecules in MPP(+)-lesioned rat brain.
abstract: The tissue distribution of interleukin-2 (IL-2) in normal and 1-methyl-4-phenyl-pyridinium (MPP+)-lesioned brains of rats was investigated. Intrastriatal administration of MPP+ caused visible damage in the vicinity of the injected region two weeks after injection. Autoradiography of the tissue section with anti-IL-2 antibodies plus trace amounts of radiolabeled IL-2 showed that the antibodies treatment elicited a selective radiolabeling of the brain tissues localized at the MPP(+)-lesioned region but not at normal cryo-sliced sections. Addition of radiolabeled IL-2 alone or normal rabbit immunoglobulins did not show any labeling effect. These autoradiographic imaging results suggest that there is an accumulation of cells bearing IL-2-like molecules at the MPP(+)-induced lesion sites.
authors.affiliation: Division of Cytokine Biology, Food and Drug Administration, Bethesda, MD 20892.
authors.forename: S M
authors.initials: SM
authors.lastname: Liang
authors.forename: C M
authors.initials: CM
authors.lastname: Liang
authors.forename: C C
authors.initials: CC
authors.lastname: Chiueh
mesh_terms: D015655:1-Methyl-4-phenylpyridinium / Q000494:pharmacology*; D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D001923:Brain Chemistry; D007376:Interleukin-2 / Q000032:analysis* / Q000378:metabolism; D008297:Male; D010302:Parkinson Disease, Secondary / Q000139:chemically induced / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D015375:Receptors, Interleukin-2 / Q000032:analysis / Q000378:metabolism
chemical_list: D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D015655:1-Methyl-4-phenylpyridinium