title: Effect of acute experimental influenza A virus pneumonia on concentration of alpha 1-acid glycoprotein in mouse serum.
abstract: In mice, the mean serum concentration of the acute-phase reactant alpha 1-acid glycoprotein increased 34-48% over 14 days following experimental induction of pneumonitis by intranasal inoculation of influenza A virus. Inoculation of undiluted (hemagglutination titer 640) and 10(-1) dilution of virus was followed by development of maximum concentrations of alpha 1-acid glycoprotein in serum at seven days, of 334 micrograms/ml, compared to a concentration in control mice inoculated with irradiated inactivated virus of 225 micrograms/ml (P = 0.002). Infection with 10(-2) virus yielded a peak serum alpha 1-acid glycoprotein of 301 micrograms/ml at four days, 34% higher than in control mice at four days (P = 0.04). There were no differences in alpha 1-acid glycoprotein concentrations among virus-infected mice. Influenza A virus pneumonitis was confirmed histologically, by virus isolation, and by serologic testing, but no inoculum-dependent differences were observed. On day 7, there was a direct relationship demonstrated between the severity of pneumonitis evaluated histologically and the serum alpha 1-acid glycoprotein concentration (r = 0.50; P less than 0.02). Influenza A pneumonia in mice is associated with increased concentrations of alpha 1-acid glycoprotein in serum; the increase may be directly related to the severity of the pulmonary inflammation.
authors.affiliation: Department of Medical Microbiology, University of Manitoba, Winnipeg, Canada.
authors.forename: W
authors.initials: W
authors.lastname: Wong
authors.forename: F Y
authors.initials: FY
authors.lastname: Aoki
authors.forename: A D
authors.initials: AD
authors.lastname: Friesen
authors.forename: D S
authors.initials: DS
authors.lastname: Sitar
authors.forename: R J
authors.initials: RJ
authors.lastname: Weselake
mesh_terms: D000208:Acute Disease; D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000276:immunology; D006384:Hemagglutination; D009980:Influenza A virus / Q000302:isolation & purification; D008168:Lung / Q000382:microbiology / Q000473:pathology; D008297:Male; D051379:Mice; D009961:Orosomucoid / Q000032:analysis*; D009976:Orthomyxoviridae Infections / Q000097:blood* / Q000473:pathology; D009994:Osmolar Concentration; D011024:Pneumonia, Viral / Q000097:blood* / Q000473:pathology
chemical_list: D000906:Antibodies; D009961:Orosomucoid