[Isolation of monocytes from peripheral blood by gradient centrifugation on Lymphoprep in a hypertonic medium].
The author describes a new method of isolation of monocytes from peripheral blood, involving hypertonization of the blood and isolation of monocytes by centrifuging above a gradient of hypertonic Lymphoprep. The method makes it possible to obtain a monocyte suspension with at least 85% cells; there are no B lymphocytes in the suspension and the monocytes are not damaged. The quality of the thus obtained monocytes was tested by examination with monocytic sera and with HLA-DR antibodies, whereby the test was concurrently made with monocytes obtained by adherence and with B lymphocytes.
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D002469:Cell Separation / Q000295:instrumentation / Q000379:methods; D002498:Centrifugation; D003601:Cytotoxicity Tests, Immunologic; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D006982:Hypertonic Solutions; D009000:Monocytes / Q000276:immunology
D006684:HLA-DR Antigens; D006982:Hypertonic Solutions
Izolace monocytů z periferní krve centrifugací nad gradientem Lymphoprep v hypertonickém prostredí.