Development of enterobacterium-specific oligonucleotide probes based on the surface-exposed regions of outer membrane proteins.
Outer membrane proteins of members of the family Enterobacteriaceae consist of conserved membrane-spanning segments and hypervariable, surface-exposed regions. We demonstrate that the hypervariable DNA segments corresponding to the surface-exposed regions of these proteins can be used to develop specific DNA probes for the identification of members of the family Enterobacteriaceae.
Spierings
G
G
Institute of Molecular Biology and Medical Biotechnology, State University of Utrecht, The Netherlands.
Hofstra
H
H
Huis in'T Veld
J
J
Hoekstra
W
W
Tommassen
J
J
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001483:Base Sequence; D003429:Cross Reactions; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics*; D004755:Enterobacteriaceae / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011237:Predictive Value of Tests; D013045:Species Specificity
D001425:Bacterial Outer Membrane Proteins; D015342:DNA Probes; D004269:DNA, Bacterial