Effect of various factors and substrates on the growth of a human hepatoblastoma cell line, HuH-6 in a serum-free medium.
The effect of various factors and substrates on the growth of a human hepatoblastoma cell line, HuH-6, which was inoculated at low density in a serum-free medium was examined. Several supplements were required to enhance cell growth of HuH-6. These included cholera toxin (CT), glucagon (Glu) and selenium (Se). Type IV collagen (C-IV) provided the most conductive environment tested for cell growth. These results suggest that CT, Glu, Se, and C-IV are important stimulators for the continuous growth of HuH-6 in a serum-free medium at low density.
Tokiwa
T
T
Division of Pathology, Okayama University Medical School, Japan.
Kusaka
Y
Y
Muraoka
A
A
Monteiro
A N
AN
Sato
J
J
D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D003094:Collagen / Q000494:pharmacology; D005934:Glucagon / Q000494:pharmacology; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D008114:Liver Neoplasms, Experimental / Q000473:pathology*; D051379:Mice; D051381:Rats; D012643:Selenium / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D003094:Collagen; D005934:Glucagon; D012643:Selenium