A procedure for eliminating interferences in the lowry method of protein determination.
A modification of the Lowry assay for the quantitative protein measurement in the presence of interfering materials has been developed. The method is based on a precipitation with a single-phase hexane:isopropanol solvent system and later resuspension of protein pellets with sodium dodecyl sulfate and deoxycholate. The new procedure eliminates the interference caused by Triton X-100, phospholipids, or dithiothreitol providing yields higher than 95% and seems to be especially suitable for protein determination on membrane preparations in samples with small volumes and/or very low protein concentrations.
Rodríguez-Vico
F
F
Department of Biochemistry and Molecular Biology, University of Granada, Spain.
Martínez-Cayuela
M
M
García-Peregrín
E
E
Ramírez
H
H
D000818:Animals; D002623:Chemistry Techniques, Analytical / Q000379:methods; D002645:Chickens; D004229:Dithiothreitol; D008832:Microchemistry / Q000379:methods; D017830:Octoxynol; D010743:Phospholipids; D011092:Polyethylene Glycols; D011506:Proteins / Q000032:analysis*; D012997:Solvents
D010743:Phospholipids; D011506:Proteins; D012997:Solvents; D011092:Polyethylene Glycols; D017830:Octoxynol; D004229:Dithiothreitol