Visualization of the cytoskeleton in Leydig cells in vitro. Effect of luteinizing hormone and cytoskeletal disrupting drugs.
Effect of LH, vinblastine and cytochalasin B on the cytoskeleton of cultured Leydig cells was investigated using monoclonal antibodies and fluorescence microscopy. After LH addition and treatment with cytoskeletal disrupting drugs, three main effects were observed: 1) increase of androgen level secreted by cultured mouse Leydig cells, 2) changes of cell-shape towards regular and rounded, 3) increase of delta 5,3 beta-HSD activity. The results are discussed in respect to possible involvement of cytoskeleton in the regulation of steroidogenesis.
Bilińska
B
B
Department of Animal Physiology, Jagiellonian University, Krakow, Poland.
D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D003571:Cytochalasin B / Q000494:pharmacology*; D003599:Cytoskeleton / Q000187:drug effects* / Q000502:physiology; D007985:Leydig Cells / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D007986:Luteinizing Hormone / Q000494:pharmacology*; D008297:Male; D051379:Mice; D013739:Testosterone / Q000378:metabolism; D014747:Vinblastine / Q000494:pharmacology*
D003571:Cytochalasin B; D013739:Testosterone; D014747:Vinblastine; D007986:Luteinizing Hormone; D015096:3-Hydroxysteroid Dehydrogenases