Neuropeptide Y- and somatostatin-like immunoreactivities in ganglioneuroma, ganglioneuroblastoma and neuroblastoma.
Neuropeptide Y (NPY)- and somatostatin (SS)-like immunoreactivities (LI) were investigated in tumor tissues of one ganglioneuroma (GN), 3 ganglioneuroblastomas (GNB) and one neuroblastoma (NB) by radioimmunoassay. NPY-LI was detected from all 5 tumor tissues (16.4-1247 pmol/g wet tissue). Sephadex G-50 column chromatography and reverse phase high performance liquid chromatography (HPLC) revealed that most of the NPY-LI in tumor extracts was eluted in an identical position to synthetic human NPY except one GNB (case 2). In this case, most of the NPY-LI was eluted in a higher molecular weight region than synthetic human NPY in Sephadex G-50 column chromatography and in a more hydrophobic position in HPLC. SS-LI was detected from 4 tumor extracts except one GNB (case 2) (21.3-787 pmol/g wet tissue). Sephadex G-25 column chromatography and reverse phase HPLC revealed that SS-LI in tumor extracts was eluted just after the void volume and then in the same positions as SS-28 and SS-14. These results suggest that NPY, SS-14 and SS-28 exist in tumor tissues of GN, GNB and NB, and most of the NPY-LI in one GNB was a higher molecular and more hydrophobic form of NPY-LI.
Takahashi
K
K
Second Department of Internal Medicine, Tohoku University School of Medicine, Miyagi, Japan.
Mouri
T
T
Itoi
K
K
Sone
M
M
Hayashi
Y
Y
Murakami
O
O
Ohneda
M
M
Ohi
R
R
Yoshinaga
K
K
D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005729:Ganglioneuroma / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D009447:Neuroblastoma / Q000276:immunology / Q000378:metabolism*; D009478:Neuropeptide Y / Q000276:immunology / Q000378:metabolism*; D010455:Peptides / Q000276:immunology / Q000378:metabolism*; D011863:Radioimmunoassay
D009478:Neuropeptide Y; D010455:Peptides; C029993:somatostatin-like peptides