The detection of group-specific component from urine samples.
The detection of group-specific component (Gc) from serum and bloodstains has been widely used in the forensic laboratory. A recent increase in substituted or adulterated urine samples in various drug screening programs has necessitated methods to determine the donor. This paper discusses the detection of Gc from urine samples. The samples were concentrated and applied to ultrathin polyacrylamide gel and focused for 150 min. This method separates the samples into the three common phenotypes found in all human populations. A nitrocellulose membrane blotting technique was used to detect the Gc bands. Serum and urine samples were collected from each individual and were typed for Gc. Urine samples tested after 6 months of storage (4 degrees C) were still readable. This method provides the forensic laboratory with an additional test from a body fluid which, until recently, provided little information.
Mills
P R
PR
United States Army Criminal Investigation Laboratory, Ft. Gillem, Forest Park, GA 30050-5000.
Chase
M G
MG
D015925:Cryopreservation; D006801:Humans; D015151:Immunoblotting; D007525:Isoelectric Focusing; D010641:Phenotype; D014809:Vitamin D-Binding Protein / Q000097:blood / Q000652:urine*
D014809:Vitamin D-Binding Protein