[Immunoelectron microscopic observation of membranous nephropathy in children using protein A-gold complex method].
The ultrastructural localization of immunoglobulins (IgG, IgA, IgM), complement component (C3c), or fibrinogen-related antigen (FRA) was investigated on 5 biopsy samples from 5 children with idiopathic MN or lupus nephritis using protein A-gold (PAG) complex method. The immunoreactivity of IgG was essentially confined to the mesangial and subepithelial electron-dense deposit (EDD) in all of 5 children, and more intense staining of IgG was observed in 3 children with stage II of MN than those with stage I and stage III of MN. Double immunocytochemical staining showed the same distribution of both IgG and IgM or both IgG and FRA in subepithelial EDD in 2 of 5 children. These findings suggest that IgG deposits are associated with the formation of subepithelial EDD in MN, and raise the possibility that IgM and FRA deposits may result from entrapment and/or immunological reaction.
Kawahara
S
S
Matsunaga
T
T
Sakamoto
S
S
Taira
K
K
Kamitsuji
H
H
Nakajima
M
M
D000293:Adolescent; D000936:Antigen-Antibody Complex / Q000032:analysis; D000941:Antigens / Q000032:analysis*; D002648:Child; D015932:Complement C3c / Q000032:analysis*; D005260:Female; D015433:Glomerulonephritis, Membranous / Q000276:immunology* / Q000378:metabolism; D006046:Gold; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D007150:Immunohistochemistry / Q000379:methods; D007668:Kidney / Q000276:immunology; D008181:Lupus Nephritis / Q000276:immunology / Q000378:metabolism; D008297:Male; D013205:Staphylococcal Protein A
D000936:Antigen-Antibody Complex; D000941:Antigens; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A; C023456:fibrinogen-related antigen; D006046:Gold; D015932:Complement C3c