Molecular species analysis of phospholipids by negative ion fast atom bombardment mass spectrometry: application of surface precipitation technique.
Eight phospholipidic classes from bovine brain cortex and soybean were prepared and purified by preparative high-performance and liquid chromatography, and their molecular species were identified by negative ion fast atom bombardment mass spectrometry using the 'surface precipitation' method. Its main advantages are: (i) clear-cut and abundant diagnostic ions for structural elucidation of the species; (ii) fragments characteristic of the main fatty acids, the polar head-group and the molecule frame in phospholipids; (iii) less background caused by the liquid matrix.
Chen
S
S
Mario Negri Institute for Pharmacological Research, Bergamo, Italy.
Benfenati
E
E
Fanelli
R
R
Kirschner
G
G
Pregnolato
F
F
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D008244:Lysophosphatidylcholines / Q000032:analysis; D008246:Lysophospholipids / Q000032:analysis; D013058:Mass Spectrometry; D010713:Phosphatidylcholines / Q000032:analysis; D010714:Phosphatidylethanolamines / Q000032:analysis; D010716:Phosphatidylinositols / Q000032:analysis; D010743:Phospholipids / Q000032:analysis*; D013025:Glycine max / Q000032:analysis; D013109:Sphingomyelins / Q000032:analysis
D008244:Lysophosphatidylcholines; D008246:Lysophospholipids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010716:Phosphatidylinositols; D010743:Phospholipids; D013109:Sphingomyelins; C025059:lysophosphatidylserine