title: Matrix collagen of devitalized bone is resistant to osteoclastic bone resorption.
abstract: The resorption of devitalized bone by isolated osteoclasts in culture was studied by scanning electron microscopy. Osteoclasts attached to the bone and resorbed mineral but left most of matrix collagen undigested in the resorption pits. Postculture-treatment of bone substrate with collagenase completely removed the matrix collagen in the resorption pits, whereas trypsin did not. The results suggest that native matrix collagen is resistant to osteoclastic attack in this in vitro model using devitalized bone.
authors.affiliation: Harvard School of Dental Medicine, Boston, MA.
authors.forename: H
authors.initials: H
authors.lastname: Shimizu
authors.forename: S
authors.initials: S
authors.lastname: Sakamoto
authors.forename: M
authors.initials: M
authors.lastname: Sakamoto
mesh_terms: D000818:Animals; D001857:Bone Matrix / Q000032:analysis* / Q000378:metabolism / Q000648:ultrastructure; D001862:Bone Resorption / Q000378:metabolism*; D001842:Bone and Bones / Q000032:analysis / Q000378:metabolism* / Q000648:ultrastructure; D002478:Cells, Cultured; D003094:Collagen / Q000032:analysis / Q000378:metabolism*; D008252:Macaca fascicularis; D008854:Microscopy, Electron; D010010:Osteoclasts / Q000378:metabolism* / Q000502:physiology / Q000648:ultrastructure
chemical_list: D003094:Collagen