Specific killing of mouse leukemic cells with ricin A-chain immunotoxin.
Monoclonal IgM antibody against L1210V leukemia was coupled with ricin A-chain using N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP) as a cross--linking agent. The coniugate had potent concentration--dependent cytotoxicity against L1210V, L1210 and RL male 1 cells being completely non toxic to EL-4, P388, RPC-5 and mouse bone marrow cells. The minimum time required for killing L120V leukemia cells was 30h of in vitro exposure, at a concentration 10(-6) M (as assessed by trypan blue test). However, 1h contact of L1210V cells with immunotoxin was sufficient to completely inhibit proliferation of leukemic cells subsequently inoculated into compatible mice. The toxicity could be potentiated by addition of NH4Cl, that shortened minimum exposure time to 18h and 45 min respectively.
Wiedłocha
A
A
Department of Tumor Immunology, Polish Academy of Sciences, Wrocław.
Salwa
J
J
Paprocka
M
M
Steuden
I
I
Radzikowski
C
C
D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D000970:Antineoplastic Agents / Q000493:pharmacokinetics / Q000627:therapeutic use*; D002460:Cell Line; D003432:Cross-Linking Reagents; D003601:Cytotoxicity Tests, Immunologic; D000922:Immunotoxins / Q000627:therapeutic use*; D037102:Lectins / Q000493:pharmacokinetics / Q000627:therapeutic use*; D007942:Leukemia, Experimental / Q000188:drug therapy* / Q000276:immunology; D008297:Male; D051379:Mice; D037121:Plant Lectins; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000970:Antineoplastic Agents; D003432:Cross-Linking Reagents; D000922:Immunotoxins; D037102:Lectins; D037121:Plant Lectins; C017707:Ricinus communis agglutinin-1; D000643:Ammonium Chloride