title: Clinical importance of erythrocyte polyamine level determination during bone marrow transplantation in children.
abstract: Previous studies have shown that red blood cell (RBC) spermidine (Spd) and spermine (Spm) concentrations appear to be a reliable index of cell proliferation. Our aim was to study the RBC polyamine level evolution (Spd and Spm) in bone marrow (BM) transplanted children. Because of our interest in the finding of an early blood criteria of BM regeneration, our study was based upon the chemotherapy - induced post-transplant aplasia period. After BM transplantation, two main periods were observed: the first (A-period) corresponded to abnormally low Spd levels. This period ended with an increasing amount of Spd reaching normal values and with an inversion in the Spd/Spm ratio which became greater than 1. The second (B) period was usually linked to abnormally high RBC Spd concentrations and a Spd/Spm ratio greater than 1. The end of the B-period was characterized by an increase in the granulocyte count (reaching 0.5 X 10(9) cells/l). Since the A- and B-periods are considered as a post-transplant aplasia period (only according to leukocyte count) and since normal RBC Spd levels occurred 14 days (SD = 4) after BM transplantation and 16 days (SD = 12) before granulocyte rise, these data led us to consider erythrocyte polyamine levels to be an earlier biological criteria of bone marrow engraftment than the number of circulating granulocytes.
authors.affiliation: Unité d'Hémato-oncologie Pédiatrique, Hôpital Sud, Rennes, France.
authors.forename: C
authors.initials: C
authors.lastname: Bergeron
authors.forename: F
authors.initials: F
authors.lastname: Bouet
authors.forename: V
authors.initials: V
authors.lastname: Quemener
authors.forename: R
authors.initials: R
authors.lastname: Havouis
authors.forename: L A
authors.initials: LA
authors.lastname: Martin
authors.forename: E
authors.initials: E
authors.lastname: Le Gall
authors.forename: J P
authors.initials: JP
authors.lastname: Moulinoux
mesh_terms: D000293:Adolescent; D015415:Biomarkers / Q000097:blood*; D016026:Bone Marrow Transplantation; D002455:Cell Division; D002648:Child; D002675:Child, Preschool; D004912:Erythrocytes / Q000032:analysis*; D005260:Female; D006801:Humans; D007223:Infant; D007945:Leukemia, Lymphoid / Q000097:blood / Q000601:surgery*; D008223:Lymphoma / Q000097:blood / Q000601:surgery*; D008297:Male; D009447:Neuroblastoma / Q000097:blood / Q000601:surgery*; D012016:Reference Values; D013095:Spermidine / Q000097:blood*; D013096:Spermine / Q000097:blood*; D013724:Teratoma / Q000097:blood / Q000601:surgery*; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous
chemical_list: D015415:Biomarkers; D013096:Spermine; D013095:Spermidine