title: Preservation of immature hematopoietic progenitor cells responding to interleukin 3 in marrow treated with 4-hydroperoxycyclophosphamide.
abstract: The toxic effects of 4-hydroperoxycyclophosphamide (4HC) on different human hematopoietic progenitor cells were determined. Day 7 colonies supported by granulocyte colony stimulating factor (G-CSF), day 10 colonies supported by granulocyte-macrophage colony stimulating factor (GM-CSF) were counted. Approximately 2 X 10(2) granulocyte-macrophage colonies per 5 X 10(4) bone marrow (BM) mononuclear cells were formed in each assay system. A combination of interleukin 3 (IL3) and erythropoietin (EPO) induces 3 types of day 14 colonies; erythroid burst-forming units (BFU-E, 40 +/- 29/5 X 10(4) BM cells), granulocytes-macrophage colony forming units (CFU-GM, 143 +/- 29/5 X 10(4) cells and mixed colonies (CFU-GEMM, 24 +/- 13/5 X 10(4) cells). After treatment with 4HC, all the colonies were reduced. However, the recovery rate of day 14 colonies supported by the combination of IL3 and EPO (mean 12.1%) was significantly higher than the recovery rate of both day 7 colonies supported by G-CSF (mean 1.0%) and day 10 colonies supported by GM-CSF (mean 4.0%). The recovery rate of colonies supported by IL3 and EPO as also higher than that of day 14 colonies supported by GM-CSF and EPO. Immature pluripotent hematopoietic progenitor cells supported by IL3 and EPO appeared less sensitive to 4HC treatment than those supported by G-CSF and GM-CSF. Colony forming assays using IL3 may be useful in order to predict the hematological reconstitution after autologous bone marrow transplantation with 4HC treated graft.
authors.affiliation: Department of Pediatrics, Tohoku University, Sendai.
authors.forename: N
authors.initials: N
authors.lastname: Minegishi
authors.forename: M
authors.initials: M
authors.lastname: Minegishi
authors.forename: S
authors.initials: S
authors.lastname: Tuchiya
authors.forename: T
authors.initials: T
authors.lastname: Konno
mesh_terms: D016026:Bone Marrow Transplantation; D003520:Cyclophosphamide / Q000031:analogs & derivatives* / Q000633:toxicity; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D007377:Interleukin-3 / Q000494:pharmacology*
chemical_list: D007377:Interleukin-3; D003520:Cyclophosphamide; C011272:perfosfamide