Superabsorbent polymers--media for the enzymatic detection of ethyl alcohol in urine.
Superabsorbent polymers are shown to be an excellent medium for the detection of enzymes used in enzyme assays. They offer increased sensitivity of 2-10 times over conventional blotter medium. Their usefulness is demonstrated with a quick, inexpensive, visual test for alcohol in urine with sensitivity approaching 0.001% (w/v). Alcohol levels in 500 random urine samples were determined and the levels ranged from 0.06 to less than 0.001%, with the majority of positives being near 0.005%.
Kidwell
D A
DA
Chemistry Division, Naval Research Laboratory, Washington, D.C. 20375-5000.
D000042:Absorption; D000426:Alcohol Dehydrogenase / Q000378:metabolism; D055598:Chemical Phenomena; D002621:Chemistry; D000431:Ethanol / Q000378:metabolism / Q000652:urine*; D006801:Humans; D009243:NAD / Q000378:metabolism; D055095:Optics and Photonics; D010209:Paper; D011108:Polymers; D013053:Spectrophotometry; D015813:Substance Abuse Detection / Q000379:methods*
D011108:Polymers; D009243:NAD; D000431:Ethanol; D000426:Alcohol Dehydrogenase