Age influence on sucrose hydrolysis and on monosaccharide absorption along the small intestine of rat.
The consequences of aging on the hydrolysis and absorption of hexoses was assessed in vitro using everted intestinal segments. Glucose and fructose were given either as a mixture of free monosaccharides or as a disaccharide solution (sucrose). The jejuno-ileum of 3- and 24-month-old rats was everted and divided into four equal segments. For each segment, the mucosal medium contained either sucrose (140 mM) or an equimolar mixture of glucose and fructose (70 mM). Monosaccharide concentrations in the mucosal ans serosal media were measured after 1 h of incubation at 37 degrees C. In the young adult, glucose absorption was enhanced when given as sucrose. In contrast, in the aged rat, free glucose or glucose released from sucrose hydrolysis were similarly absorbed. Independently of age, fructose was better absorbed when provided in a mixture of free monosaccharides. The intestinal segments (and especially the ileum), of the aged animals exhibited higher abilities to hydrolyse sucrose and to absorb monosaccharides indicating a normal or increased intestinal hydrolytic activity and absorptive capacity for dietary sugars.
Darmenton
P
P
Laboratories Cernep-Synthelabo, Strasbourg, France.
Raul
F
F
Doffoel
M
M
Wessely
J Y
JY
D000375:Aging / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D005632:Fructose / Q000378:metabolism; D006868:Hydrolysis; D007408:Intestinal Absorption / Q000502:physiology*; D007421:Intestine, Small / Q000502:physiology*; D008297:Male; D009005:Monosaccharides / Q000493:pharmacokinetics*; D051381:Rats; D011919:Rats, Inbred Strains; D013395:Sucrose / Q000378:metabolism*
D009005:Monosaccharides; D005632:Fructose; D013395:Sucrose