title: Determination of glucose-6-phosphate dehydrogenase isoenzymes of human erythrocytes by a thin-layer PAG-IEF procedure.
abstract: A procedure of thin-layer PAG-IEF was employed to separate glucose-6-phosphate dehydrogenase (G6PD) isoenzymes of human erythrocytes into 15-16 bands in a pH range between 4.7 and 6.5. The G6PD isoenzymes were scanned with a dual-wavelength thin-layer chromatoscanner and the relative enzyme content of each band was calculated. The method possesses the merits of simplicity, high sensitivity, rapidity and high reproducibility.
authors.affiliation: Human University of Medical Science, Changsha, China.
authors.forename: C X
authors.initials: CX
authors.lastname: Nie
authors.forename: S T
authors.initials: ST
authors.lastname: Tong
authors.forename: D X
authors.initials: DX
authors.lastname: Chen
mesh_terms: D002855:Chromatography, Thin Layer / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004912:Erythrocytes / Q000201:enzymology*; D005954:Glucosephosphate Dehydrogenase / Q000097:blood* / Q000302:isolation & purification; D006801:Humans; D007527:Isoenzymes / Q000097:blood* / Q000302:isolation & purification
chemical_list: D007527:Isoenzymes; D005954:Glucosephosphate Dehydrogenase