Improved separation of lipid esters by thin-layer chromatography.
Methods for the separation of ethyl- and 2-chloroethyl esters of fatty acids using argentation thin-layer chromatography (TLC) and linear and circular reversed-phase TLC are described. An argentation TLC method for the separation of cholesterol esters is also described. The separation is based on the degree of unsaturation in the fatty acid moiety. In all instances good and reproducible resolution was achieved by unidirectional single developments at room temperature with reduced analysis times (9 min for argentation TLC, 7 min for circular reversed-phase TLC).
Bhat
H K
HK
Division of Biochemistry, University of Texas Medical Branch, Galveston 77550.
Ansari
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GA
D002788:Cholesterol Esters / Q000032:analysis; D002855:Chromatography, Thin Layer; D005227:Fatty Acids / Q000032:analysis*; D012015:Reference Standards
D002788:Cholesterol Esters; D005227:Fatty Acids