Neuroblastoma-glioma hybrid cells contain clonidine-displacing substance.
Clonidine-displacing substance (CDS) isolated from bovine brain potently inhibits clonidine binding and elicits contraction of gastric smooth muscle. We sought to determine if CDS was contained in neuron-like clonal cells (neuroblastoma X glioma hybrid NG108-15). Extracts were prepared from osmotically shocked P2 fractions of NG108-15 cells. One unit of CDS, as defined by a [3H]p-aminoclonidine radioreceptor assay using bovine frontal cortex membranes, was obtained from each 1.3 million cells processed. CDS isolated from NG108-15 cells was biologically active on gastric smooth muscle. NG108-15 cells may serve as a model system for the study of this endogenous clonidine-like ligand.
Ernsberger
P
P
Division of Neurobiology, Cornell University Medical College, New York, NY 10021.
Meeley
M P
MP
Reis
D J
DJ
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D003000:Clonidine / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors* / Q000302:isolation & purification / Q000378:metabolism; D005748:Gastric Fundus / Q000378:metabolism; D005910:Glioma / Q000378:metabolism*; D006822:Hybrid Cells / Q000378:metabolism*; D051379:Mice; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism; D009447:Neuroblastoma / Q000378:metabolism*; D011869:Radioligand Assay
C016986:apraclonidine; C055140:clonidine-displacing substance; D003000:Clonidine