title: Oxalate, phosphate and sulphate determination in serum and urine by ion chromatography.
abstract: A rapid method for the determination of phosphate, sulphate and oxalate in serum by ion chromatography is described. Serum is deproteinized through a Centrifree filter by centrifugation and the ultrafiltrate directly injected into an ion chromatograph equipped with an anion exchange column and a conductivity detector. By this procedure the sample is not diluted and even small amounts of oxalate in biological fluids can be detected. Mean serum concentrations found in healthy individuals are: phosphate 1.07 mmol/l; sulphate 0.35 mmol/l; oxalate 21.02 mumols/l. Phosphate, sulphate and oxalate contents were also determined in urine from healthy individuals. Values found in serum and urine are in good agreement with those previously reported.
authors.affiliation: Dipartimento di Scienze e Tecnologie Biochimiche e Biometria, Università dell'Aquila, Rome, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Politi
authors.forename: R
authors.initials: R
authors.lastname: Chiaraluce
authors.forename: V
authors.initials: V
authors.lastname: Consalvi
authors.forename: N
authors.initials: N
authors.lastname: Cerulli
authors.forename: R
authors.initials: R
authors.lastname: Scandurra
mesh_terms: D002852:Chromatography, Ion Exchange / Q000379:methods*; D006801:Humans; D010070:Oxalates / Q000032:analysis* / Q000097:blood / Q000652:urine; D010710:Phosphates / Q000032:analysis* / Q000097:blood / Q000652:urine; D012015:Reference Standards; D013431:Sulfates / Q000032:analysis* / Q000097:blood / Q000652:urine
chemical_list: D010070:Oxalates; D010710:Phosphates; D013431:Sulfates