title: Molecular species analysis of phospholipids by negative ion fast atom bombardment mass spectrometry: application of surface precipitation technique.
abstract: Eight phospholipidic classes from bovine brain cortex and soybean were prepared and purified by preparative high-performance and liquid chromatography, and their molecular species were identified by negative ion fast atom bombardment mass spectrometry using the 'surface precipitation' method. Its main advantages are: (i) clear-cut and abundant diagnostic ions for structural elucidation of the species; (ii) fragments characteristic of the main fatty acids, the polar head-group and the molecule frame in phospholipids; (iii) less background caused by the liquid matrix.
authors.affiliation: Mario Negri Institute for Pharmacological Research, Bergamo, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Chen
authors.forename: E
authors.initials: E
authors.lastname: Benfenati
authors.forename: R
authors.initials: R
authors.lastname: Fanelli
authors.forename: G
authors.initials: G
authors.lastname: Kirschner
authors.forename: F
authors.initials: F
authors.lastname: Pregnolato
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D008244:Lysophosphatidylcholines / Q000032:analysis; D008246:Lysophospholipids / Q000032:analysis; D013058:Mass Spectrometry; D010713:Phosphatidylcholines / Q000032:analysis; D010714:Phosphatidylethanolamines / Q000032:analysis; D010716:Phosphatidylinositols / Q000032:analysis; D010743:Phospholipids / Q000032:analysis*; D013025:Glycine max / Q000032:analysis; D013109:Sphingomyelins / Q000032:analysis
chemical_list: D008244:Lysophosphatidylcholines; D008246:Lysophospholipids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010716:Phosphatidylinositols; D010743:Phospholipids; D013109:Sphingomyelins; C025059:lysophosphatidylserine