[Luminescence of activated phagocytes].
By means of microscope-cytofluorometry "Luman-13" intensive proper luminescence of neutrophilic granulocytes and monocytes in blood and pus, that reduce nitroblue tetrazolium has been investigated. The luminescence is localized in the cytoplasmic areas free from formazan; formazan itself does not demonstrate any luminescence. Two types of luminescence have been revealed: dark blue-blue and yellow-green. According to the luminescence spectral analysis stimulation with radiation at the wave length 360 nm produces luminescence of the I type with its maximum in the area of 450-470 nm and 520-535 nm. When stimulation with light at the wave length is 460 nm, the luminescence spectrum++ of the II type possesses the main maximum at 535 nm. Luminescence spectra of neutrophilic granulocytes and monocytes are similar. The luminescence stripe in the area 450-470 nm results from reduced pyridinenucleotides, luminescence with the wave length 520-535 nm--from oxidized flavoproteins.
Slavinskiĭ
A A
AA
D002478:Cells, Cultured; D003470:Culture Media; D004579:Electron Transport / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D009580:Nitroblue Tetrazolium / Q000494:pharmacology; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D003470:Culture Media; D009580:Nitroblue Tetrazolium
Liuminestsentsiia aktivirovannykh fagotsitov.