Measurement of an intracellular pH rise after fertilization in crab eggs using 31P-NMR.
The effect of fertilization upon the intracellular pH, pHi, in crab ovulated eggs was examined by 31P-NMR. The pHi values were obtained from the chemical shift differences between the phosphoarginine PA resonance and the inorganic phosphate Pi resonance. The detection of the Pi peak was accomplished by Hahn spin-echo experiments in order to cancel the broad signal arising from phosphoproteins which overlaps the Pi signal. The average pHi of the unfertilized unactivated eggs was 6.55 and a rise of 0.12 pH unit occurred after fertilization.
Hervé
M
M
Service de Biophysique, CEN Saclay, Gif-sur-Yvette, France.
Goudeau
M
M
Neumann
J M
JM
Debouzy
J C
JC
Goudeau
H
H
D000818:Animals; D003386:Brachyura; D005260:Female; D005306:Fertilization; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008433:Mathematics; D009865:Oocytes / Q000502:physiology*; D010758:Phosphorus
D010758:Phosphorus