title: Interconvertible hairpin structure found in the replication origin of phage G4 single-stranded DNA.
abstract: We found a synthetic GCGAAAGC fragment with a mobility greater than that of other oligodeoxyribonucleotides in gel electrophoresis to take on a stable hairpin structure possessing two terminal G-C base pairs. The GCGAAAGC sequence is also found in the replication origin of phage G4 single-stranded DNA, but the hairpin structure originally proposed differs from that of the GCGAAAGC fragment we have studied. Possibility of rearrangement of the secondary structure in the replication origin of phage G4 was examined in relation to its replication initiation mechanism.
authors.affiliation: Department of Industrial Chemistry, Faculty of Engineering, University of Tokyo, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Hirao
authors.forename: Y
authors.initials: Y
authors.lastname: Nishimura
authors.forename: M
authors.initials: M
authors.lastname: Ishida
authors.forename: K
authors.initials: K
authors.lastname: Watanabe
authors.forename: K
authors.initials: K
authors.lastname: Miura
mesh_terms: D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012093:Replicon
chemical_list: D004277:DNA, Single-Stranded; D004279:DNA, Viral