title: Body impedance is largely dependent on the intra- and extra-cellular water distribution.
abstract: Electrical impedance measurements were performed in fasting blood samples and analysed in relation to packed cell volume and calculated intra- and extra-cellular water distribution. The total blood impedance was shown to be strongly dependent on the ratio of intra-cellular water to total water (r = 0.97, P less than 0.0001). In a group of 515 subjects, with a large variation in age and body composition, the relation between the body impedence corrected for fat-free mass and body height (the specific body impedance) and the calculated ratio of intra-cellular water to total body water, was found to be similar to that in blood. From these observations a regression model was developed and applied to body compositional data of several groups of subjects before and after weight loss caused by water losses. It was possible to calculate at a group level the losses of intra- and extra-cellular water, which confirms the applicability of the model. It is concluded that the validity of the predicted fat-free mass or total body water from body impedance is largely dependent on the water distribution in the measured subjects. This means that age- and sex-specific prediction formulas have to be used for the assessment of the body composition and that the bio-electrical impedance method is only with caution applicable in subjects with a disturbed water distribution as in oedema, pregnancy and dehydration.
authors.affiliation: Department of Human Nutrition, Agricultural University, Wageningen, The Netherlands.
authors.forename: P
authors.initials: P
authors.lastname: Deurenberg
authors.forename: K
authors.initials: K
authors.lastname: van der Kooy
authors.forename: R
authors.initials: R
authors.lastname: Leenen
authors.forename: F J
authors.initials: FJ
authors.lastname: Schouten
mesh_terms: D000328:Adult; D000886:Anthropometry; D001834:Body Water / Q000502:physiology*; D004553:Electric Conductivity / Q000502:physiology*; D005110:Extracellular Space / Q000502:physiology*; D005260:Female; D006400:Hematocrit; D006801:Humans; D008297:Male; D014882:Water-Electrolyte Balance / Q000502:physiology*