title: A novel isocratic HPLC method to separate and quantify acetanilide and its hydroxy aromatic derivatives: 2-, 3- and 4-hydroxyacetanilide (paracetamol or acetaminophen).
abstract: Reverse-phase high performance liquid chromatography on a microBondapak C-18 Column has been used to separate and quantify acetanilide and its aromatic monohydroxy derivatives in the 2-, 3- and 4- positions. Separation was achieved within 22 min by using an isocratic mixture of 2-propanol: methanol: water, 8:18:74 (v/v). This method compares very favourably with other HPLC techniques already reported to separate acetanilide from the monohydroxy aromatic derivatives.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, University of Chile, Santiago.
authors.forename: J
authors.initials: J
authors.lastname: Mancilla
authors.forename: E
authors.initials: E
authors.lastname: Valdes
authors.forename: L
authors.initials: L
authors.lastname: Gil
mesh_terms: D000082:Acetaminophen / Q000302:isolation & purification*; D000083:Acetanilides / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000083:Acetanilides; D012997:Solvents; D000082:Acetaminophen