Inhibition of L-arginine iminohydrolase (EC 3.5.3.6) from Tetrahymena thermophila by putrescine and spermidine: feedback control of polyamine biosynthesis.
L-Arginine iminohydrolase (arginine deiminase, ADI) from Tetrahymena thermophila was purified approx. 75-fold by means of gel permeation chromatography. The Km of the purified enzyme for L-arginine was 412 +/- 25 microM and L-ornithine inhibited the reaction competitively with a Ki of 985 +/- 105 microM. D-Ornithine was a weak inhibitor with a Ki of greater than 10mM. The polyamines putrescine and spermidine inhibited ADI incompetitively with a Kii of 2.8mM for putrescine and 4.3mM for spermidine. Since the concentrations required for inhibition were within the range of the normal intracellular polyamine concentrations in Tetrahymena (maximally 14mM putrescine and 4mM spermidine), it is suggested that the polyamine effects on ADI are of regulatory nature. Thus, polyamine biosynthesis in Tetrahymena thermophila is regulated not only on the level of ornithine decarboxylase activity, but also on an earlier step, the supply of ODC with substrates.
Eichler
W
W
Institut für Biochemie I der Universität Heidelberg.
D000818:Animals; D001120:Arginine / Q000378:metabolism; D005246:Feedback / Q000502:physiology; D006867:Hydrolases / Q000037:antagonists & inhibitors* / Q000302:isolation & purification; D009952:Ornithine / Q000494:pharmacology; D011700:Putrescine / Q000494:pharmacology*; D013095:Spermidine / Q000494:pharmacology*; D013768:Tetrahymena / Q000201:enzymology*
D001120:Arginine; D009952:Ornithine; D006867:Hydrolases; C019723:arginine deiminase; D013095:Spermidine; D011700:Putrescine