title: Isolation, purification and characterization of infectious hypodermal and hematopoietic necrosis virus (IHHNV) from penaeidshrimp.
abstract: A protocol was developed for the isolation and purification of the infectious hypodermal and hematopoietic necrosis virus (IHHNV) of penaeid shrimps. Cesium chloride-banded virus obtained from three sources of infected shrimps was found to have a buoyant density of 1.33 g/cm3. Also, electron microscopical studies employing negative stain revealed isometric particles having a size of 19 +/- 1 nm. Colorimetric analysis of its nucleic acid type indicated that these particles contain only RNA.
authors.affiliation: Department of Microbiology, University of Hawaii, Honolulu.
authors.forename: Y
authors.initials: Y
authors.lastname: Lu
authors.forename: P C
authors.initials: PC
authors.lastname: Loh
authors.forename: J A
authors.initials: JA
authors.lastname: Brock
mesh_terms: D000818:Animals; D002499:Centrifugation, Density Gradient; D033364:Decapoda / Q000382:microbiology*; D008854:Microscopy, Electron; D012367:RNA, Viral / Q000032:analysis; D014780:Viruses / Q000302:isolation & purification* / Q000648:ultrastructure
chemical_list: D012367:RNA, Viral