[Arg8]vasopressin-induced contractions of rabbit urinary bladder smooth muscle.
The effects of a number of neurohypophyseal hormones and analogues on the contractility of rabbit urinary bladder smooth muscle in vitro were examined. The order of potency was [Arg8]vasopressin = [Lys8]vasotocin greater than [Lys8]vasopressin greater than [Arg8]vasotocin much greater than [deamino1,D-Arg8]vasopressin greater than oxytocin much greater than pressinoic acid. The maximum tension induced by [Arg8]vasopressin was 4.4 N . cm-2 and the pD2 was 8.7. The effects of [Arg8]vasopressin were competitively antagonized by [(beta-mercapto-beta,beta-cyclopentamethylenepropionic acid)1,(O-methyl)Tyr2,Arg8]vasopressin and by [deaminopenicillamine1,Val4,D-Arg8]vasopressin with pA2s of 8.4 and 6.6, respectively. It was concluded that rabbit urinary bladder smooth muscle contains receptors for the neurohypophyseal hormones which have recognition properties similar to V1-vasopressin receptors.
Crankshaw
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Department of Obstetrics and Gynecology, McMaster University, Hamilton, Ontario, Canada.
D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects*; D010909:Pituitary Hormones, Posterior / Q000494:pharmacology; D011817:Rabbits; D013779:Tetrodotoxin / Q000494:pharmacology; D001743:Urinary Bladder / Q000187:drug effects; D014667:Vasopressins / Q000494:pharmacology; D014668:Vasotocin / Q000494:pharmacology
D010909:Pituitary Hormones, Posterior; D014667:Vasopressins; D001127:Arginine Vasopressin; D013779:Tetrodotoxin; D002217:Carbachol; D002118:Calcium; D014668:Vasotocin; D007213:Indomethacin