Establishment of IL-5-dependent early B cell lines by long-term bone marrow cultures.
We established two different IL-5-dependent Ly1+ early B cell lines in long-term bone marrow culture system. One of them (J-87) is stromal cell (ST2) dependent and the other (T-88) is ST2 independent. Both J-87 and T-88 are B220+, Ly1+, sIgM-, Ia-, Thy1-, and IL-2R+, and respond to IL-3 and IL-5 in the presence of ST2. The T-88 can proliferate only in response to IL-5 in the absence of ST2. Southern blot analysis using JH probe revealed that configuration of IgH gene of both cell lines shows rearranged pattern. Binding assay for radiolabeled IL-5 to T-88 demonstrated that T-88 has two classes of IL-5 binding sites (low and high affinity) on the membrane. These data strongly suggest that there are IL-5-sensitive stages at both stromal cell-dependent and stromal cell-independent phases in early B cell development.
Tominaga
A
A
Department of Biology, Kumamoto University Medical School, Japan.
Mita
S
S
Kikuchi
Y
Y
Hitoshi
Y
Y
Takatsu
K
K
Nishikawa
S
S
Ogawa
M
M
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology*; D001854:Bone Marrow Cells; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D002478:Cells, Cultured; D005260:Female; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain / Q000502:physiology; D007377:Interleukin-3 / Q000502:physiology; D015848:Interleukin-5 / Q000502:physiology*; D007700:Kinetics; D051379:Mice; D008811:Mice, Inbred DBA; D010641:Phenotype; D011971:Receptors, Immunologic / Q000032:analysis; D018123:Receptors, Interleukin; D053649:Receptors, Interleukin-5
D007377:Interleukin-3; D015848:Interleukin-5; D011971:Receptors, Immunologic; D018123:Receptors, Interleukin; D053649:Receptors, Interleukin-5