title: A versiconal hemiacetal acetate converting enzyme in aflatoxin biosynthesis.
abstract: Conversion of the aflatoxin biosynthetic intermediate versiconal hemiacetal acetate (VHA) in a cell free extract of Aspergillus parasiticus ATCC 15517 is investigated. The enzymatic reaction is monitored by a method using high performance liquid chromatography (HPLC). The major product of the enzymatic reaction is a water soluble compound not chloroform-extractable at pH 7.5. The product becomes chloroform extractable upon acidification of the reaction medium and is separated and quantitated by reversed-phase HPLC. It is tentatively identified as 'versiconal hemiacetal alcohol,' which is converted to versicolorin C (VC) upon acid treatment.
authors.affiliation: Department of Environmental Toxicology, University of California, Davis 95616.
authors.forename: D P
authors.initials: DP
authors.lastname: Hsieh
authors.forename: C C
authors.initials: CC
authors.lastname: Wan
authors.forename: J A
authors.initials: JA
authors.lastname: Billington
mesh_terms: D000348:Aflatoxins / Q000096:biosynthesis*; D000880:Anthraquinones / Q000378:metabolism; D001230:Aspergillus / Q000201:enzymology*; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration
chemical_list: D000348:Aflatoxins; D000880:Anthraquinones; C027121:versiconal hemiacetal acetate; C029826:versicolorin A; D002265:Carboxylic Ester Hydrolases; C062670:versiconal hemiacetal acetate converting enzyme; C029827:versicolorin C