Effects of hydrostatic pressure on the location of PRODAN in lipid bilayers: a FT-IR study.
The effects of hydrostatic pressure on the location of 6-propionyl-2-(dimethylamino)naphthalene (PRODAN), an environmentally sensitive fluorescent probe, in phosphatidylcholine lipid bilayers have been studied by Fourier-transform infrared spectroscopy (FT-IR) over the pressure range of 0.001-25 kbar. The results derived from the PRODAN C = O stretching band, the correlation field splitting of the methylene scissoring mode, and the methylene symmetric stretching mode as well as the absorption of the naphthalene ring show that in the sample of 4% (w/w) PRODAN in dimyristoyl-L-alpha-phosphatidylcholine (DMPC) at pH 6.8, most of the PRODAN molecules are embedded in the bilayers. In contrast, at pH 3.0, PRODAN was found to reside either on the membrane surface or dispersed in water. Compared to DMPC, egg yolk phosphatidylcholine (egg PC), which contains a substantial amount of unsaturated fatty acyl chains, is more susceptible to PRODAN permeation. The present study shows that the pressure dependence of the location of PRODAN in lipid membranes is different from that of tetracaine, a local anesthetic, in lipid bilayers. The model regarding the PRODAN location in lipid bilayers derived from the present infrared data has been compared with that obtained with previous fluorescence studies.
Chong
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PL
Department of Biochemistry, Meharry Medical College, Nashville, Tennessee 37208.
Capes
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Wong
P T
PT
D015081:2-Naphthylamine / Q000031:analogs & derivatives / Q000032:analysis*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D005456:Fluorescent Dyes / Q000032:analysis*; D005583:Fourier Analysis; D006874:Hydrostatic Pressure; D008051:Lipid Bilayers / Q000032:analysis*; D009281:Naphthalenes / Q000032:analysis*; D010539:Permeability; D010713:Phosphatidylcholines; D013055:Spectrophotometry, Infrared
D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D009281:Naphthalenes; D010713:Phosphatidylcholines; C038517:prodan; D015081:2-Naphthylamine