Induction of suppressor macrophages by 12-O-tetradecanoylphorbol-13-acetate in phorbol ester-sensitive (SENCAR) and resistant (B6C3F1) mice.
Following topical application of 8 micrograms 12-O-tetradecanoylphorbol-13-acetate (TPA) twice in one week, the ability of splenic macrophages (M phi s) isolated from phorbol ester-sensitive (SENCAR) and resistant (B6C3F1) mice to suppress the phytohemagglutinin (PHA)-induced lymphocyte blastogenesis and NK activity mediated by spleen cells from naive animals was determined. In B6C3F1 mice, suppression of lectin-induced lymphocyte blastogenesis was mediated by M phi s from TPA-dosed animals. Alternatively, in TPA-dosed SENCAR mice, induction of M phi s suppressive to lectin responses was not apparent. In addition, suppressor M phi s did not mediate the decreased splenic natural killer (NK) activity that is characteristically observed in TPA-dosed SENCAR mice. Therefore, it is proposed that the decreased PHA responsiveness and NK activity observed in vivo in TPA-dosed SENCAR mice may be the result of a decreased proportion of lectin-responding T cells and NK cells in the spleen as a result of proliferation of inflammatory cell precursors.
Chuthaputti
A
A
Department of Pharmacology and Toxicology, School of Pharmacy and Pharmacal Sciences, Purdue University, West Lafayette, IN 47907.
Updyke
L W
LW
Yoon
H L
HL
Pfeifer
R W
RW
Yim
G K
GK
D000818:Animals; D004351:Drug Resistance; D005260:Female; D007165:Immunosuppression Therapy; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D010703:Phorbol Esters / Q000494:pharmacology; D013045:Species Specificity; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D010703:Phorbol Esters; D013755:Tetradecanoylphorbol Acetate