Amino acid sequence studies on cyanogen bromide peptides of chicken caldesmon which bind to calmodulin.
Three major calmodulin-binding cyanogen bromide peptides (fragments A, B, and D) were isolated from chicken gizzard muscle caldesmon and their amino acid sequences were determined. The molecular masses of fragments A, B, and D were estimated to 16, 12, and 9 kDa, respectively, by SDS-urea polyacrylamide gel electrophoresis. Fragment A was composed of 102 amino acid residues and contained homoserine at the C terminus. The amino acid sequence from the 37th residue of fragment A corresponds to the N-terminal sequence of the 15 kDa peptide which was obtained by thrombin digestion [Mornet, D., Audemard, E., & Derancourt, J. (1988) Biochem. Biophys. Res. Commun. 154, 564-571]. Thrombin 15 kDa peptide binds to F-actin but does not bind to calmodulin. Thus the N-terminal 36 residues and the C-terminal part from the 37th residue of fragment A are supposed to bind to calmodulin and F-actin, respectively. The sequences of fragments B and D were identical, but fragment D was composed of 64 amino acid residues and ended with tryptophan, whereas fragment B was of 98 or 99 amino acid residues and ended with proline. Both fragments B and D are supposed to be the C-terminal peptides of chicken caldesmon. Fragment B had heterogeneous sequences at the C-terminal region. These results can explain the reported heterogeneity of chicken caldesmon in charge and molecular mass.
Takagi
T
T
Biological Institute, Faculty of Science, Tohoku University, Miyagi.
Yazawa
M
M
Ueno
T
T
Suzuki
S
S
Yagi
K
K
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002148:Calmodulin-Binding Proteins; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D003488:Cyanogen Bromide; D008969:Molecular Sequence Data; D010446:Peptide Fragments; D011487:Protein Conformation; D012697:Serine Endopeptidases
D000596:Amino Acids; D002148:Calmodulin-Binding Proteins; D010446:Peptide Fragments; D012697:Serine Endopeptidases; C036813:lysyl endopeptidase; D003488:Cyanogen Bromide