title: An inhibitory effect of RGD peptide on protein-priming reaction of bacteriophages phi 29 and M2.
abstract: The amino acid sequence, arginine-glycine-aspartic acid (RGD), found in some cell adhesive proteins, is a recognition signal for the receptor protein. It is interesting that we have found the RGD sequence in terminal protein (TP) of bacteriophages phi 29 and M2 near an amino acid, the serine residue at 232, covalently linked to the terminal nucleotide of their DNAs. At the initiation of protein-primed DNA replication, TP is essential for the recognition of replication machinery containing DNA polymerase and primer protein (PP; PP becomes TP upon linking the first nucleotide, and hence the primary structure of TP is the same as that of PP). Synthetic peptide RGD specifically inhibited transfection of phi 29 and M2. The target of the RGD peptide is shown to be TP by marker rescue experiments, suggesting that a receptor for the RGD sequence exists in TP. Furthermore, the peptide inhibited the in vitro protein-priming reaction of DNA replication. We propose that the RGD sequence of PP and a putative receptor on TP is utilized for the molecular recognition initiating DNA replication.
authors.affiliation: Life Science Institute, Sophia University, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kobayashi
authors.forename: K
authors.initials: K
authors.lastname: Matsumoto
authors.forename: S
authors.initials: S
authors.lastname: Misawa
authors.forename: K
authors.initials: K
authors.lastname: Miura
authors.forename: H
authors.initials: H
authors.lastname: Hirokawa
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus; D001435:Bacteriophages / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D004261:DNA Replication / Q000187:drug effects*; D004279:DNA, Viral / Q000187:drug effects*; D005819:Genetic Markers; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000494:pharmacology*; D014162:Transfection; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D004279:DNA, Viral; D005819:Genetic Markers; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid