title: Determination of leucine enkephalin and methionine enkephalin in equine cerebrospinal fluid by microbore high-performance liquid chromatography and capillary zone electrophoresis coupled to tandem mass spectrometry.
abstract: The performance of microbore high-performance liquid chromatography and capillary zone electrophoresis, both equipped with on-line tandem mass spectrometric detection capability, was evaluated critically for the determination of endogenous amounts of leucine enkephalin and methionine enkephalin in equine cerebrospinal fluid. Using an identical sample clean-up and enrichment procedure, capillary zone electrophoresis-mass spectrometry is limited in its concentration detection capacity owing to its much smaller injection volume. Leucine enkephalin was identified in post-mortem equine cerebrospinal fluid at the 1-5 ng/ml level by liquid chromatography-mass spectrometry.
authors.affiliation: Equine Drug Testing and Toxicology Program, New York State College of Veterinary Medicine, Cornell University, Ithaca 14850.
authors.forename: W M
authors.initials: WM
authors.lastname: Mück
authors.forename: J D
authors.initials: JD
authors.lastname: Henion
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004586:Electrophoresis / Q000379:methods; D004743:Enkephalin, Leucine / Q000134:cerebrospinal fluid*; D004744:Enkephalin, Methionine / Q000134:cerebrospinal fluid*; D006736:Horses; D013058:Mass Spectrometry
chemical_list: D004744:Enkephalin, Methionine; D004743:Enkephalin, Leucine