A multipurpose vector for the study of transcriptional control.
To facilitate the insertion of transcriptional control regions next to a reporter gene, plasmid vectors containing multiple cloning sites next to the cat have been constructed. These vectors also contain features which make their use convenient for the construction of deletions in the inserted transcriptional control regions, as well as for the direct sequencing of the deletion series produced. The vectors have been constructed such that the control region cassette (and the deletions produced) may be easily removed with or without the reporter gene for placement into transgenic mice. The system's utility has been demonstrated by deletion analysis of a chicken myosin heavy chain-encoding gene promoter.
Robbins
J
J
Department of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine, OH 45267-0575.
Subramaniam
A
A
Gulick
J
J
D000818:Animals; D002645:Chickens; D003001:Cloning, Molecular / Q000379:methods*; D005796:Genes; D005822:Genetic Vectors; D015879:Myosin Subfragments / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D014158:Transcription, Genetic
D015879:Myosin Subfragments