[Use of sebacic acid for identifying anaerobic bacteria using gas phase chromatography].
Volatile short-chain fatty acids formed as end products of metabolism of 25 anaerobic bacterial strains isolated from different clinical specimens and collections were analysed by GLC. Strains were previously identified using microscopic, cultural and biochemical tests. Gas-chromatograms of acids (volatile fatty acid profiles) were obtained with a sebacic acid packed column. The potential application of the GLC technique for the rapid and accurate identification of these organisms is discussed.
Larrinaga
A
A
Instituto Nacional de Farmacología y Bromatología, Buenos Aires, Argentina.
Predari
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SC
Frade
A H
AH
D001421:Bacteria, Anaerobic / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000175:diagnosis; D002849:Chromatography, Gas; D003652:Decanoic Acids; D003998:Dicarboxylic Acids; D005232:Fatty Acids, Volatile / Q000032:analysis*
D003652:Decanoic Acids; D003998:Dicarboxylic Acids; D005232:Fatty Acids, Volatile; C011107:sebacic acid
Uso del ácido sebácico en la identificación de bacterias anaerobias por cromatografía en fase gaseosa (CGL).