title: Permeability of milk protein antigens across the intestinal epithelium in vitro.
abstract: Degradations by proteolytic enzymes and intestinal epithelial permeability represent two major drawbacks to the transfer of food protein antigens to blood. These steps were studied in vitro for the milk protein antigens beta-lactoglobulin (beta-Lg), alpha-Lactalbumin (alpha-La) and beta-casein (beta-cas). Pepsin-trypsin hydrolysis and permeability in isolated rabbit ileum in Ussing chamber were suited by ELISA and radiolabelled-protein measurement. Pepsin-trypsin hydrolysis showed an increasing resistance in the order beta-cas less than alpha-La less than beta-Lg. The rate of absorption of the antigenic proteins by isolated rabbit ileum was in the same order, and the rate of absorption of the whole proteins (degraded and antigenic forms) was significantly higher for beta-Lg than for alpha-La and beta-cas. These results suggest a selective intestinal permeability for milk protein antigens. This selectivity is probably important in the mechanism of food protein sensitization via the oral route.
authors.affiliation: INSERM U 290, fonctions intestinales, métabolisme et nutrition, hôpital Saint-Lazare, Paris, France.
authors.forename: D
authors.initials: D
authors.lastname: Marcon-Genty
authors.forename: D
authors.initials: D
authors.lastname: Tomé
authors.forename: A M
authors.initials: AM
authors.lastname: Dumontier
authors.forename: O
authors.initials: O
authors.lastname: Kheroua
authors.forename: J F
authors.initials: JF
authors.lastname: Desjeux
mesh_terms: D000818:Animals; D002364:Caseins / Q000276:immunology / Q000378:metabolism*; D002417:Cattle; D005512:Food Hypersensitivity / Q000276:immunology / Q000378:metabolism; D006868:Hydrolysis; D007082:Ileum / Q000378:metabolism*; D007408:Intestinal Absorption / Q000276:immunology; D007413:Intestinal Mucosa / Q000378:metabolism*; D007768:Lactalbumin / Q000276:immunology / Q000378:metabolism*; D007782:Lactoglobulins / Q000276:immunology / Q000378:metabolism*; D008892:Milk / Q000276:immunology / Q000378:metabolism; D011817:Rabbits
chemical_list: D002364:Caseins; D007782:Lactoglobulins; D007768:Lactalbumin