[Effective method of simultaneous extraction from the urine and purification of tissue kallikrein and acid-stable trypsin inhibitor].
A simple preparative procedure is developed for simultaneous isolation from urine of tissue kallikrein and acid stable trypsin inhibitor. The procedure involved adsorption of these proteins on chitosan at pH 5-6 and the subsequent elution with 1 N NH4OH, which enabled to obtain the enzyme and inhibitor with a yield of 80-90% and to purify 10-fold each of these components. Use of chitosan facilitated and simplified distinctly the large scale isolation from urine of the kallikrein and trypsin inhibitor, required for medicinal and diagnostic purposes.
Lobareva
L S
LS
Platonova
L V
LV
Rabinovich
S E
SE
Paliulina
M V
MV
Paskhina
T S
TS
D000328:Adult; D002686:Chitin / Q000031:analogs & derivatives; D048271:Chitosan; D002846:Chromatography, Affinity; D006023:Glycoproteins / Q000302:isolation & purification / Q000652:urine*; D006801:Humans; D007202:Indicators and Reagents; D007610:Kallikreins / Q000302:isolation & purification / Q000652:urine*; D008297:Male; D014361:Trypsin Inhibitors / Q000302:isolation & purification / Q000652:urine*
D006023:Glycoproteins; D007202:Indicators and Reagents; D014361:Trypsin Inhibitors; D002686:Chitin; D048271:Chitosan; D007610:Kallikreins; C028665:urinastatin
Effektivnyĭ sposob odnovremennogo izvlecheniia iz mochi i ochistki tkanevogo kallikreina i kislotostabil'nogo ingibitora tripsina.