[Mutagenic activity in urine from smokers detected by sister chromatid exchange (SCE) of peripheral lymphocytes].
Mutagenic activity in urine from smokers was detected by SCE. Human peripheral lymphocytes were treated with 50 microliters urine (contain 0.1 mg creatinine) from 63 smokers and 34 non-smokers for SCE analysis. The results showed that there was significantly higher SCE frequencies (7.82 +/- 1.12/cell) of lymphocytes treated with urine of smokers as compared with those of non-smokers (6.49 +/- 1.12/cell) (P less than 0.001). However, the mutagenicity in urine of smokers who smoked 20 cigarettes/day was similar to those of smokers on 10 cigarettes/day. Intake of mutagens from food might be responsible for the low level mutagens in urine of some non-smokers. This test may provide a sensitive, economical bioassay for evaluating cancer risk in smokers.
Hu
G G
GG
Cancer Institute, Chinese Academy of Medical Sciences, Beijing.
D000328:Adult; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure; D008297:Male; D008875:Middle Aged; D009152:Mutagenicity Tests; D012307:Risk Factors; D012854:Sister Chromatid Exchange; D012907:Smoking / Q000009:adverse effects / Q000652:urine*