title: Synthesis of a thymosin beta 4-like peptide, deacetyl-thymosin beta Xen4, and its restorative effect on depressed lymphocyte blastogenic response to phytohemagglutinin (PHA) in uremic patients.
abstract: An analog of thymosin beta Xen4 isolated from oocytes of Xenopus laevis, deacetyl-thymosin beta Xen4, was synthesized by assembling 6 peptide fragments, followed by deprotection with 1 M trifluoromethanesulfonic acid-thioanisole (molar ratio, 1:1) in trifluoroacetic acid in the presence of dimethylselenium. Finally, the deprotected peptide was incubated with dithiothreitol to reduce sulfoxide on the methionine side chain. The synthetic tritetracontapeptide was found to have a restoring effect on the impaired blastogenic response of T-lymphocytes isolated from uremic patients.
authors.forename: T
authors.initials: T
authors.lastname: Abiko
authors.forename: H
authors.initials: H
authors.lastname: Sekino
mesh_terms: D000595:Amino Acid Sequence; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation / Q000187:drug effects*; D008969:Molecular Sequence Data; D010835:Phytohemagglutinins; D013947:Thymosin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology; D014511:Uremia / Q000276:immunology*; D000099201:Thymosin beta(4)
chemical_list: D010835:Phytohemagglutinins; D013947:Thymosin; D000099201:Thymosin beta(4); C062363:deacetylthymosin beta(4)(Xen)