title: Glutathione metabolism in cultured Sertoli cells and spermatogenic cells from hamsters.
abstract: Isolated spermatocytes and spermatids from hamsters contained a large amount of glutathione (GSH) (approximately 40 and 30 nmol GSH/mg protein, respectively), but showed a spontaneous decrease of GSH content during prolonged incubation (t1/2 approximately 35 h). Incubation of the germ cells in the presence of the glutathione biosynthesis inhibitor buthionine sulphoximine (BSO) provided evidence that the cells can perform glutathione synthesis. This synthesis, however, was not sufficient to maintain the GSH content of the isolated cells, or to restore the cellular GSH pool after depletion caused by exposure of the cells to the glutathione S-transferase substrate, diethyl maleate (DEM). Cultured Sertoli cells, containing approximately 10 nmol GSH/mg protein, had a more active BSO-sensitive GSH synthesis system. The Sertoli cells, but also tubule fragments containing Sertoli cells and germ cells, were able to restore their GSH pool after DEM-induced depletion. DEM treatment of the tubule fragments resulted in a 90% decrease of the GSH content of the spermatocytes and spermatids present within the fragments. The GSH levels of the tubule fragments and the enclosed germ cells were restored during a subsequent incubation in the absence of DEM. As indicated above, such a recovery was not observed for isolated spermatocytes and spermatids. The results illustrate the importance of Sertoli cell-germ cell interaction, and point to a role of Sertoli cells in glutathione synthesis by the germ cells.
authors.affiliation: Department of Biochemistry II, Medical Faculty, Erasmus University Rotterdam, The Netherlands.
authors.forename: P J
authors.initials: PJ
authors.lastname: Den Boer
authors.forename: P
authors.initials: P
authors.lastname: Mackenbach
authors.forename: J A
authors.initials: JA
authors.lastname: Grootegoed
mesh_terms: D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology; D019328:Buthionine Sulfoximine; D002478:Cells, Cultured; D006224:Cricetinae; D005978:Glutathione / Q000378:metabolism*; D008297:Male; D008298:Maleates / Q000494:pharmacology; D008647:Mesocricetus; D008717:Methionine Sulfoximine / Q000031:analogs & derivatives / Q000494:pharmacology; D012708:Sertoli Cells / Q000187:drug effects / Q000378:metabolism*; D013087:Spermatids / Q000187:drug effects / Q000378:metabolism*; D013090:Spermatocytes / Q000187:drug effects / Q000378:metabolism*
chemical_list: D000963:Antimetabolites; D008298:Maleates; D008717:Methionine Sulfoximine; D019328:Buthionine Sulfoximine; C014476:diethyl maleate; D005978:Glutathione