The introduction of reporter groups at multiple and/or specific sites in DNA containing phosphorothioate diesters.
DNA fragments containing phosphorothioate diesters provide nucleophilic sites which are amenable to labeling by spin labels or fluorophores. Selecting the position for an individual phosphorothioate diester allows highly specific placement of the reporter group. The substitution of a phosphorothioate diester for each and every internucleotidic phosphodiester allows the incorporation of multiple reporter groups; ideally one for each nucleoside residue. With the use of multiple fluorophores a post-assay fluorescent labeling technique has been developed which allows the detection of DNA fragments with the "naked-eye" in the low femtomolar (10(-15) moles) range.
Conway
N E
NE
Department of Chemistry, Boston College, Chestnut Hill, MA 02167.
Fidanza
J
J
McLaughlin
L W
LW
D001643:Bridged Bicyclo Compounds; D003497:Cyclic N-Oxides; D005456:Fluorescent Dyes; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D013113:Spin Labels; D013873:Thionucleotides
D001643:Bridged Bicyclo Compounds; D003497:Cyclic N-Oxides; D005456:Fluorescent Dyes; D009838:Oligodeoxyribonucleotides; D013113:Spin Labels; D013873:Thionucleotides; C029910:3-(2-iodoacetamido)-2,2,5,5-tetramethyl-1-pyrrolidinyloxyl; C028381:monobromobimane