The difference spectra of bovine kidney RNase K2 induced upon binding with nucleotides markedly differ from those of bovine pancreatic RNase.
The difference spectra obtained upon the addition of nucleotides to bovine kidney RNase, which shows 40% sequence homology with bovine pancreatic RNase, are markedly different from those of bovine pancreatic RNase. As one of the factors which possibly contribute to this difference, we examined the effect of the substitution of Phe120 in bovine pancreatic RNase by Leu in RNase K2 on the difference spectra.
Irie
M
M
Department of Microbiology, Hoshi College of Pharmacy, Tokyo.
Ohgi
K
K
Nitta
R
R
Ikeda
M
M
Ueno
M
M
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D003568:Cytidine Monophosphate / Q000378:metabolism; D007668:Kidney / Q000201:enzymology*; D007700:Kinetics; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009711:Nucleotides / Q000378:metabolism*; D010179:Pancreas / Q000201:enzymology*; D012259:Ribonuclease, Pancreatic / Q000378:metabolism*; D012260:Ribonucleases / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D014542:Uridine Monophosphate / Q000378:metabolism
D009711:Nucleotides; C063259:ribonuclease K2; D014542:Uridine Monophosphate; D012260:Ribonucleases; D012259:Ribonuclease, Pancreatic; D003568:Cytidine Monophosphate