Suppression of mast cell colony formation by a low molecular weight fraction of fetal calf bone marrow extract.
A semi-purified fraction extracted from fetal calf bone marrow was previously shown to contain a tetrapeptide N-Ac-Ser-Asp-Lys-Pro which inhibits in mice, hematopoietic stem cell entry into the cell cycle. This peptide however, did not exhibit any effect on either IL-3 nor GM-CSF dependent cell growth. We report herein that a semi-purified fraction also contains another activity which is antagonistic to IL-3. Addition of the fraction in vitro decreased IL-3 dependent mast cell colony formation. Growth of IL-3 dependent cell lines (DA-1 and FDC-P2) was also suppressed. No effect was observed in the same dose range on granulocyte-macrophage colony formation nor on IL-3 independent cell growth.
Lenfant
M
M
Institut de Chimie des Substances Naturelles, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France.
Sotty
D
D
Wdzieczak-Bakala
J
J
Itoh
K
K
Shirata
K
K
Sugimoto
K
K
Mori
K J
KJ
D000818:Animals; D001853:Bone Marrow / Q000502:physiology*; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D005260:Female; D007377:Interleukin-3 / Q000494:pharmacology; D008407:Mast Cells / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D008809:Mice, Inbred C3H; D013234:Stem Cells / Q000187:drug effects*; D014020:Tissue Extracts / Q000494:pharmacology
D007377:Interleukin-3; D014020:Tissue Extracts