Production, characterization, and utility of monoclonal antibodies which react with a novel chimeric glycoprotein of human respiratory syncytial virus termed FG.
Primary immunization of mice with recombinant vaccinia virus expressing the F or G glycoprotein of human respiratory syncytial virus followed by an intravenous boost with crude FG chimeric glycoprotein resulted in the generation of hybridomas each specific for either the F or G portion of FG. Characterization of each MAb was determined following binding to various viral and glycoprotein antigens, by immunoprecipitation, by competition binding and by subclass determination. Relative affinity was determined for each MAb following inhibition of binding by ammonium thiocyanate.
Brideau
R J
RJ
Upjohn Company, Kalamazoo, MI.
Walters
R R
RR
Wathen
M W
MW
D000818:Animals; D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D006023:Glycoproteins / Q000235:genetics / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D012136:Respiratory Syncytial Viruses / Q000235:genetics / Q000276:immunology*; D014616:Vaccinia virus / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D011994:Recombinant Proteins; D014764:Viral Proteins