Stimulatory effect of calcitonin on bone formation in tissue culture.
The present investigation was undertaken to clarify the in vitro effect of synthetic [Asu1,7]eel calcitonin (CT) on bone metabolism in tissue culture. Calvaria were removed from weanling rats (3-week-old male) and cultured for periods up to 96 h in Dulbecco's Modified Eagle Medium supplemented with antibiotics and bovine serum albumin. The experimental cultures contained 1.0 to 100 ng/ml CT. All cultures were incubated at 37 degrees C in 5% CO2-95% air. Bone calcium content was increased significantly by the presence of 10 and 100 ng/ml CT. This increase was blocked by the presence of 10(-6) M cycloheximide or 10(-7) M actinomycin D. Bone alkaline phosphatase activity was significantly increased by the presence of 100 ng/ml CT for 48 and 96 h. Bone acid phosphatase activity was not altered significantly by CT (1-100 ng/ml). The incorporation of [3H]proline into the acid-insoluble residues of bone tissue was significantly increased by the presence of CT (1-100 ng/ml) for 96 h. This increase was completely blocked by the presence of 10(-7) M cycloheximide. Bone DNA content was significantly raised by the presence of 10 and 100 ng/ml CT for 96 h. Furthermore, the culture with CT (10 and 100 ng/ml) produced a significant decrease in glucose concentration in the medium. Also, CT (10 and 100 ng/ml) stimulated the production of pyruvic acid from bone tissue. These results suggest that CT had a direct stimulatory effect on bone formation and mineralization in vitro, and that the hormone stimulates energy metabolism in bone cells.
Yamaguchi
M
M
Department of Environmental Biochemistry and Toxicology, School of Pharmaceutical Sciences, University of Shizuoka, Japan.
Uto
M
M
Matsui
R
R
D000818:Animals; D001846:Bone Development / Q000187:drug effects*; D001842:Bone and Bones / Q000187:drug effects / Q000201:enzymology; D002116:Calcitonin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D046508:Culture Techniques; D003513:Cycloheximide / Q000494:pharmacology; D004247:DNA / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D008297:Male; D011506:Proteins / Q000187:drug effects; D011773:Pyruvates / Q000378:metabolism; D019289:Pyruvic Acid; D051381:Rats; D011919:Rats, Inbred Strains
D011506:Proteins; D011773:Pyruvates; D019289:Pyruvic Acid; D002116:Calcitonin; D004247:DNA; D003513:Cycloheximide; D005947:Glucose; D002118:Calcium; C012755:elcatonin