title: Regional difference in airway epithelial response to neutrophil elastase: tracheal secretory cells discharge and recover in hamsters that develop bronchial secretory-cell metaplasia.
abstract: Human neutrophil elastase (HNE) causes secretory granule discharge and conversion of many Clara cells to mucous cells in hamster bronchi. We investigated whether the trachea responds to HNE in a similar manner because of its abundance of Clara cells. By light microscopy, the tracheal epithelium of animals exposed to a single intratracheal injection of HNE was normal at 21 days, although bronchial secretory-cell metaplasia (SCM) was present. An ultrastructural differential cell count showed no increase in the proportion of granulated secretory cells in HNE-treated animals at 8 and 21 days postinjection compared to saline or untreated controls. At 2 h, the percentage of granulated secretory cells was lower and that of granulated secretory cells was higher in HNE-treated animals than in controls. The HNE-treated animals had fewer secretory granules per cell profile and more surface undulation than controls. By 1 day, the differential cell count and number of granules per cell profile were normal. Saline did not affect the differential cell count or granule number at any time. Ultrastructural study of untreated trachea disclosed the same three types of Clara cell that are found in the bronchus, but their frequencies, with one exception, are significantly different in the two regions. We conclude that HNE acts as a secretagogue in both trachea and bronchus but that an amount of enzyme sufficient to cause bronchial SCM does not induce a similar lesion in trachea. Heterogeneity of Clara cell types in hamster airways may explain the regional variation in secretory-cell modulation by HNE.
authors.affiliation: Pulmonary Center, Boston University School of Medicine, MA 02118.
authors.forename: T G
authors.initials: TG
authors.lastname: Christensen
authors.forename: R
authors.initials: R
authors.lastname: Breuer
authors.forename: E C
authors.initials: EC
authors.lastname: Lucey
authors.forename: P J
authors.initials: PJ
authors.lastname: Stone
authors.forename: G L
authors.initials: GL
authors.lastname: Snider
mesh_terms: D000818:Animals; D001980:Bronchi / Q000473:pathology*; D006224:Cricetinae; D003594:Cytoplasmic Granules / Q000473:pathology; D004847:Epithelial Cells; D004848:Epithelium / Q000502:physiology / Q000648:ultrastructure; D006801:Humans; D008297:Male; D008647:Mesocricetus; D008679:Metaplasia; D009504:Neutrophils / Q000201:enzymology*; D009928:Organ Specificity / Q000502:physiology; D010196:Pancreatic Elastase / Q000378:metabolism*; D014132:Trachea / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure
chemical_list: D010196:Pancreatic Elastase