title: Antibody-dependent cellular cytotoxicity of vascular endothelium: characterization and pathogenic associations in systemic sclerosis.
abstract: Ten sera from 48 patients with systemic sclerosis were found to be capable of producing cytotoxicity of human umbilical venous and arterial endothelium when co-cultured with peripheral blood mononuclear cells. Fractionation of sera on Ultrogel and the preparation of monomeric IgG by ion exchange and affinity chromatography suggested that the cytotoxicity was mediated by anti-endothelial antibodies capable of pre-sensitizing target cells in a mechanism that resembled antibody-dependent cellular cytotoxicity. These anti-endothelial antibodies together with C1q-binding immune complexes and anti-cardiolipin antibodies were found in 18 of 28 patients so investigated, suggesting that multiple immunological mechanisms may be involved in the pathogenesis of the vascular lesion of systemic sclerosis.
authors.affiliation: University Department of Medicine, Northern General Hospital, Sheffield, England.
authors.forename: C M
authors.initials: CM
authors.lastname: Holt
authors.forename: N
authors.initials: N
authors.lastname: Lindsey
authors.forename: J
authors.initials: J
authors.lastname: Moult
authors.forename: R G
authors.initials: RG
authors.lastname: Malia
authors.forename: M
authors.initials: M
authors.lastname: Greaves
authors.forename: A
authors.initials: A
authors.lastname: Hume
authors.forename: N R
authors.initials: NR
authors.lastname: Rowell
authors.forename: P
authors.initials: P
authors.lastname: Hughes
mesh_terms: D000920:Antibody-Dependent Cell Cytotoxicity; D001323:Autoantibodies / Q000097:blood; D002308:Cardiolipins / Q000276:immunology; D002478:Cells, Cultured; D015922:Complement C1q / Q000378:metabolism; D004730:Endothelium, Vascular / Q000276:immunology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012595:Scleroderma, Systemic / Q000209:etiology / Q000276:immunology*; D014469:Umbilical Arteries / Q000276:immunology; D014471:Umbilical Veins / Q000276:immunology; D014841:von Willebrand Factor / Q000032:analysis
chemical_list: D001323:Autoantibodies; D002308:Cardiolipins; D014841:von Willebrand Factor; D015922:Complement C1q