title: Effect of dimethylsulfoxide on human gliomas: correlations between the nuclear magnetic resonance spectra and the transformed phenotypes of the tumor cells.
abstract: Prolonged in vitro induction of six established human glioma cell lines with dimethylsulfoxide (DMSO) generated an adherent human fibroblastoid phenotype. The development of contact-inhibited cell growth coincided with the decreased colony-forming potential of these cells in semisolid medium and with the reduction or elimination of tumorigenicity when transplanted in athymic nude mice. These DMSO-induced changes persisted for at least 19 passages after removal of the inducer from the medium. High-resolution natural-abundance 13C nuclear magnetic resonance spectroscopy showed specific spectral differences between the cell lines with more or less malignant transformed phenotypes: the glioma cells with a higher degree of tumorigenicity and colony-forming potential exhibited more intense myoinositol signals than those with the more benign phenotype.
authors.affiliation: Division of Neurology, Milton S. Hershey Medical Center, Pennsylvania State University, Hershey.
authors.forename: L
authors.initials: L
authors.lastname: Geder
authors.forename: K R
authors.initials: KR
authors.lastname: Metz
authors.forename: J
authors.initials: J
authors.lastname: Towfighi
authors.forename: J L
authors.initials: JL
authors.lastname: Mikus
authors.forename: G D
authors.initials: GD
authors.lastname: Williams
authors.forename: A J
authors.initials: AJ
authors.lastname: Gaydos
authors.forename: J W
authors.initials: JW
authors.lastname: Kreider
mesh_terms: D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D005910:Glioma; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008819:Mice, Nude; D010641:Phenotype; D014407:Tumor Cells, Cultured / Q000032:analysis / Q000166:cytology / Q000187:drug effects*
chemical_list: D004121:Dimethyl Sulfoxide