[Characteristics of DNA sequences in the sites of permanent attachment to the nuclear matrix located in the vicinity of replication initiation site].
The permanent DNA attachment sites to the nuclear matrix in the domain of chicken alpha-globin genes originally found in erythrocyte nuclei are shown to exist in sperm and cultured fibroblast cells too. Short fragments of permanently attached to the nuclear matrix DNA have been cloned and sequenced. A primary structure of a 1.7 k.b. fragment from 5'-region of chicken alpha-globin gene domain containing both replication origin and permanent attachment site has been determined. A region possessing homologies with papovaviral replication origins and putative mammalian ARS elements has been found on the 1.7 k.b. fragment. A region containing short internal repeats and GC-rich motifs has also been found. Similar motifs were observed in several of the cloned short fragments of DNA permanently attached to the nuclear matrix.
Kalandadze
A G
AG
Bushara
S A
SA
Vasetskiĭ
E S
ES
Razin
S V
SV
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002645:Chickens; D004247:DNA / Q000235:genetics* / Q000378:metabolism; D004261:DNA Replication; D004912:Erythrocytes / Q000378:metabolism; D005347:Fibroblasts; D005914:Globins / Q000235:genetics*; D008969:Molecular Sequence Data; D015530:Nuclear Matrix / Q000378:metabolism*; D012091:Repetitive Sequences, Nucleic Acid
D005914:Globins; D004247:DNA
Kharakteristika posledovatel'nosteĭ DNK v uchastkakh permanentnogo prikrepleniia DNK k iadernomu skeletu, sosedstvuiushchikh s uchastkami nachala replikatsii.