title: Partial purification, and some properties and reactivities of cetraxate benzyl ester hydrochloride-hydrolyzing enzyme.
abstract: Debenzylating enzyme from Aspergillus niger enzyme (commercial crude cellulase) catalyzes the hydrolysis of cetraxate benzyl ester hydrochloride (2), a precursor of the antiulcer agent (1). The enzyme was highly purified by three kinds of chromatographies (hydrophobic, ion exchange, gel filtration) with a recovery of 36%. The content of the debenzylating enzyme was about 0.1% in the crude cellulase, but the enzyme showed no cellulase activity. The purified enzyme was inactivated by Hg2+, and diisopropyl phosphorofluoridate (DFP). It was a monomer with a molecular weight of about 35,000, and its isoelectric point was estimated to be 5.3. It showed a debenzylating activity for the phenylpropionic acid benzyl ester moiety of various benzyl ester derivatives, and the benzyl ester of phenylalanine or that of tyrosine was also well hydrolyzed.
authors.forename: H
authors.initials: H
authors.lastname: Kuroda
authors.forename: A
authors.initials: A
authors.lastname: Miyadera
authors.forename: A
authors.initials: A
authors.lastname: Imura
authors.forename: A
authors.initials: A
authors.lastname: Suzuki
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology*; D002265:Carboxylic Ester Hydrolases / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D003509:Cyclohexanecarboxylic Acids / Q000302:isolation & purification* / Q000378:metabolism*; D013379:Substrate Specificity; D014148:Tranexamic Acid / Q000031:analogs & derivatives / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: D003509:Cyclohexanecarboxylic Acids; C063344:cetraxate benzyl ester; C000665:cetraxate; D014148:Tranexamic Acid; D002265:Carboxylic Ester Hydrolases; C063611:cetraxate benzyl ester HCl hydrolyzing enzyme