Micropreparative capillary zone electrophoresis of recombinant human interleukin-3.
Micropreparative capillary zone electrophoresis of recombinant human interleukin-3 (rhIL-3) in untreated fused silica is presented. Results show that nanogram quantities of rhIL-3 can be collected off the capillary and then identified by amino acid sequencing and SDS-gel electrophoresis.
Hecht
R I
RI
Molecular and Cell Biology Department, G.D. Searle, Monsanto Co., St. Louis, Mo 63198.
Coleman
J F
JF
Morris
J C
JC
Stover
F S
FS
Demarest
C
C
D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007377:Interleukin-3 / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000302:isolation & purification
D007377:Interleukin-3; D011994:Recombinant Proteins