Leinamycin, a new antitumor antibiotic from Streptomyces: producing organism, fermentation and isolation.
Leinamycin (DC 107) is newly discovered antitumor antibiotic with an unusual 1,3-dioxo-1,2-dithiolane structure. Five different producing strains were isolated from soils collected in Japan during 1985-1988 and were taxonomically assigned as Streptomyces. Fermentation studies indicate: Leinamycin was unstable in culture broth. A chemically defined medium could be designed for a preferable production. Streptomyces sp. S-140 grew on medium supplemented with Zn2+ and high porus polymer resin and accumulated 32 micrograms/ml of leinamycin. Improved isolation methods are described along with identification of mikamycin A co-produced with leinamycin by the strain S-140.
Hara
M
M
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Japan.
Asano
K
K
Kawamoto
I
I
Takiguchi
T
T
Katsumata
S
S
Takahashi
K
K
Nakano
H
H
D000903:Antibiotics, Antineoplastic / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D007769:Lactams; D018942:Macrolides; D013302:Streptomyces / Q000032:analysis; D013844:Thiazoles; D013871:Thiones
D000903:Antibiotics, Antineoplastic; D007769:Lactams; D018942:Macrolides; D013844:Thiazoles; D013871:Thiones; C059000:leinamycin