Hepatic level of mRNA in relation to polymorphism and strain difference in expression of cytochrome P-450g.
Hepatic level of mRNA for P-450g was determined in male and female rats of Sprague-Dawley, ACI and Fischer strains using a specific oligonucleotide probe. The mRNA level in female Sprague-Dawley and Fischer rats was undetectable in accordance with a very low or undetectable level of P-450g protein. The mRNA was detected in all male ACI rats, in which high levels of P-450g protein were detected. In Fischer male rats, mRNA for P-450g was clearly detected in the liver, but P-450g protein was not detected. Moreover, the mRNA was detected in the liver of all male Sprague-Dawley rats, irrespective of the expressed level or absence of P-450g protein. As a most plausible explanation of the observed discordance in the levels of P-450g mRNA and protein, translation of unstable protein for microheterogeneous P-450g is discussed.
Nagata
K
K
Department of Pharmacology, School of Medicine, Keio University, Tokyo, Japan.
Miyata
M
M
Shimada
M
M
Yamazoe
Y
Y
Kato
R
R
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D008099:Liver / Q000201:enzymology*; D008297:Male; D015345:Oligonucleotide Probes; D011110:Polymorphism, Genetic; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D011912:Rats, Inbred ACI; D011916:Rats, Inbred F344; D011919:Rats, Inbred Strains; D013045:Species Specificity
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D003577:Cytochrome P-450 Enzyme System