title: Determination of exifone in human plasma and urine by high-performance liquid chromatography with electrochemical detection.
abstract: A high-performance liquid chromatographic method with electrochemical detection was developed for the determination of exifone in human plasma and urine. Exifone was extracted from acidified plasma or neutralized urine with diethyl ether and the evaporated extracts were analysed on a C18 reversed-phase column. The compound was eluted in about 8 min with acetonitrile-0.3 M orthophosphoric acid (15:85, v/v) at a flow-rate of 0.9 ml/min. This method gave accurate and reproducible results; the calibration graphs were linear (r greater than 0.99) over the range of 2.8-360 nmol/l for plasma and 0.18-36 mumol/l for urine, and concentrations as low as 1 nmol/l in plasma could be quantified. These results allowed this assay to be used for determinations in single-dose pharmacokinetic studies.
authors.affiliation: LARIME, Laboratoire de Recherche pour l'Industrie du Medicament, Poitiers, France.
authors.forename: J J
authors.initials: JJ
authors.lastname: Descombe
authors.forename: G
authors.initials: G
authors.lastname: Doumont
authors.forename: M
authors.initials: M
authors.lastname: Picard
mesh_terms: D001577:Benzophenones / Q000097:blood / Q000378:metabolism* / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D006801:Humans; D011619:Psychotropic Drugs / Q000097:blood / Q000378:metabolism* / Q000652:urine; D011786:Quality Control
chemical_list: D001577:Benzophenones; D011619:Psychotropic Drugs; C052932:exifone