Sexing the human fetus and identification of polyploid nuclei by DNA-DNA in situ hybridisation in interphase nuclei.
Samples of human adult lymphocytes, fetal lymphocytes, amniotic fluid cells, and chorionic villus cells were sexed independently by cytogenetics and DNA-DNA in situ hybridisation to a tritiated Y probe. For the in situ hybridisation analysis, the presence of Y bodies (hybridisation bodies) in 100 interphase nuclei were scored after autoradiography. In all, 82/83 samples were sexed in this way (one technical failure) and 78/82 were sexed by both in situ hybridisation and cytogenetics. There was complete agreement between the two methods. There was a considerable variation (40-100%) in the percentage of interphase nuclei with a hybridisation body among the male samples, but very few nuclei from female samples showed significant hybridisation. In situ hybridisation could be used to sex the conceptus when males but not females are at risk for various X-linked genetic disorders and may also be useful for detecting 45,X/46,XY mosaicism or polyploid/diploid mosaicism. This would be particularly useful for direct preparations of chorionic villus samples, which often prove difficult to analyse cytogenetically but offer the best means of avoiding maternal contamination. Some interphase nuclei had more than one hybridisation body, and this was most commonly found among amniotic fluid cells. Comparison of sizes of nuclei with one or two hybridisation bodies strongly suggested that most of the amniotic fluid cell nuclei with two hybridisation bodies were tetraploid.
West
J D
JD
Department of Obstetrics and Gynaecology, University of Edinburgh, Scotland.
Gosden
C M
CM
Gosden
J R
JR
West
K M
KM
Davidson
Z
Z
Davidson
C
C
Nicolaides
K H
KH
D000328:Adult; D000653:Amniotic Fluid / Q000166:cytology; D002467:Cell Nucleus / Q000032:analysis* / Q000648:ultrastructure; D015342:DNA Probes; D005260:Female; D006801:Humans; D007399:Interphase; D008297:Male; D009693:Nucleic Acid Hybridization; D011123:Polyploidy; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D012732:Sex Determination Analysis; D014998:Y Chromosome
D015342:DNA Probes