[Development of an orthotopic hepatic transplantation technique in rats: what should the researcher's objectives be?].
The development of liver transplantation in man emphasises the need for more work in the field of experimental liver transplantation. For this purpose the model of orthotopic liver transplantation in the rat is very useful but difficult to establish as a safe and reproducible technique. The aim of this study concerning acquisition of the orthotopic liver transplantation model in the rat was to define precise objectives for future investigators. Two investigators without experience in microsurgery each performed 75 orthotopic liver transplantations, which were divided into five consecutive series. In each series survival times of the recipients after transplantation were analysed according to the mean duration of the operative procedure and the mean portal vein clamping time. For each investigator, mean duration of the operative procedure progressively decreased to reach a minimum at the 45th graft. From that time on, the portal vein clamping time became less than 17 minutes and a significant increase in the post operative survival time of the recipient was observed. However it was only from the sixtieth transplantation on, with stable portal vein clamping time but technical mistakes fewer that a high success rate was observed.
Michel
A
A
Filipponi
F
F
Icard
P
P
Houssin
D
D
D000714:Anastomosis, Surgical; D000818:Animals; D008099:Liver / Q000601:surgery; D016031:Liver Transplantation / Q000379:methods* / Q000401:mortality; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014680:Veins / Q000601:surgery
Mise au point de la technique de transplantation hépatique orthotopique chez le rat: quels objectifs pour l'expérimentateur?