Vascular endothelial growth factor: a new member of the platelet-derived growth factor gene family.
Using applications of the polymerase chain reaction (PCR) technique, cDNA clones have been isolated encoding bovine vascular endothelial growth factor (VEGF), a mitogen with specificity for vascular endothelial cells. Analysis of the clones indicates that VEGF can exist in two forms, probably due to alternative RNA splicing. The amino acid sequences predicted from the clones also show that VEGF shares homologies of about 21% and 24% respectively with the A and B chains of human platelet-derived growth factor (PDGF), and has complete conservation of the eight cysteine residues found in both mature PDGF chains. The homology is not reflected in function, however, since the cell types responsive to VEGF are distinct from those responsive to homo- and heterodimers of the PDGF chains.
Tischer
E
E
California Biotechnology Inc., Mountain View 94043.
Gospodarowicz
D
D
Mitchell
R
R
Silva
M
M
Schilling
J
J
Lau
K
K
Crisp
T
T
Fiddes
J C
JC
Abraham
J A
JA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002417:Cattle; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D006031:Glycosylation; D006801:Humans; D008222:Lymphokines / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010982:Platelet-Derived Growth Factor / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012326:RNA Splicing; D012689:Sequence Homology, Nucleic Acid; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D008222:Lymphokines; D010982:Platelet-Derived Growth Factor; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D004247:DNA