Development of a novel screening device permitting immunocytochemical screening of numerous culture supernatants during hybridoma production.
A novel screening device is described which permits the simultaneous immunocytochemical processing of several hundreds or even thousands of hybridoma culture supernatants. The core of the screening apparatus is a foam-coated polymer plate that carries a 96-well pattern representing a modification of the actual 96-well template. This modification permits the use of conventional 26 X 76 mm microscopy glass slides. Each of these slides carries 24 carefully arranged histological sections. One 96-well plate is thus screened by mounting four of these slides in the apparatus during the primary antibody (i.e., culture supernatant) incubation stage. At all other stages of the immunocytochemical protocol, the slides are processed in the classical way. The screening apparatus has been used during the production of monoclonal antibodies against chicken pituitary glycoprotein hormones and against bovine neurohypophyseal peptides. In both instances, it proved to be the major contributory factor in the successful production of antibodies.
Berghman
L R
LR
Laboratory of Neuroendocrinology and Immunological Biotechnology, Zoological Institute of the Catholic University, Louvain, Belgium.
Horsten
G
G
Vandesande
F
F
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D002417:Cattle; D002645:Chickens; D006825:Hybridomas / Q000276:immunology*; D007150:Immunohistochemistry / Q000295:instrumentation*; D008832:Microchemistry; D010902:Pituitary Gland / Q000166:cytology; D010904:Pituitary Gland, Posterior / Q000276:immunology; D010907:Pituitary Hormones / Q000276:immunology
D000911:Antibodies, Monoclonal; D010907:Pituitary Hormones