Identification of constitutive and steroid-dependent transactivation domains in the mouse oestrogen receptor.
We have identified two transactivation domains in the mouse oestrogen receptor whose activities depend on the target promoter. The major domain is contained within the C-terminal portion of the protein and depends upon oestrogen binding for its activity. The location and oestrogen dependence of this domain has been confirmed using chimaeric receptors containing the Lex A DNA binding domain. Although transactivation by the C-terminal domain is dependent upon ligand binding the analysis of receptor deletion mutants has demonstrated that these two functions are not entirely coincident. The second transactivation domain lies within the N-terminal region and is active in the absence of oestradiol. The differences in oestrogen requirement for the activity of the two transactivation domains may account for the partial agonist activity of certain antihormones.
Lees
J A
JA
Molecular Endocrinology Laboratory, Imperial Cancer Research Fund, London, U.K.
Fawell
S E
SE
Parker
M G
MG
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D002678:Chimera; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D004965:Estrogen Antagonists / Q000494:pharmacology; D005822:Genetic Vectors; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D015345:Oligonucleotide Probes; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D011960:Receptors, Estrogen / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D015533:Transcriptional Activation
D004965:Estrogen Antagonists; D015345:Oligonucleotide Probes; D011960:Receptors, Estrogen; D004247:DNA