13C-NMR spectroscopic studies on the glucose metabolism of Angiostrongylus cantonesis' eggs with special reference to the end-products and metabolic pathway.
13C-nuclear magnetic resonance (NMR) spectroscopy was used to identify metabolites excreted by Angiostrongylus cantonensis eggs which had been maintained aerobically in the presence of D-[13C6] glucose. Using 13C-NMR we proved that lactate, acetate and alanine originated from glucose present in the medium via glycolysis. Aminooxyacetate, an inhibitor of alanine transferase, inhibited simultaneously alanine production and the ability to take up glutamate, aspartate and valine from the medium. In addition, we demonstrated that these amino acids can serve as amino group donors of the pyruvate to alanine transamination system in the eggs.
Nishina
M
M
Department of Parasitology, Saitama Medical School, Japan.
Hori
E
E
Matsushita
K
K
Takahashi
M
M
Kato
K
K
Ohsaka
A
A
D000596:Amino Acids / Q000378:metabolism; D000625:Aminooxyacetic Acid / Q000494:pharmacology; D000801:Angiostrongylus; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002247:Carbon Isotopes; D005260:Female; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis; D007553:Isotope Labeling / Q000379:methods; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008954:Models, Biological; D010063:Ovum / Q000378:metabolism*
D000596:Amino Acids; D002247:Carbon Isotopes; D000625:Aminooxyacetic Acid; D005947:Glucose