Action of blood serum from rats with turpentine sterile inflammation on the development of CFU erythrocyte colonies. Possible role of erythropoietin.
Bone marrow CFUe mice cultures were prepared in Petri dishes and the number of full developed erythrocyte colonies were counted under various experimental conditions. In certain experiments, erythropoietin (EP = 0.4 U x ml-1 in the suspending medium) or sera from normal rats (100 microliters) or from animals with chronic turpentine sterile abscesses (60 or 100 microliters), were delivered to the CFUe colonies. The number of colonies in the group without EP was almost 5 times smaller than in the group with EP. Inasmuch as some few colonies are still able to develop, even in absence of added EP, the suggestion is advanced holding that the phenomenon may represent the effect of EP already bound to the group of cells initiating differentiation. Comparisons among all experimental groups indicate that the delivery of sera from turpentine rats to cultures containing added EP, reduced significantly the number of CFUe colonies seen in controls with EP but without inflammatory serum. It is suggested that the present findings could be explained assuming an inhibition of the influence of exogenous EP, subserved by the inflammatory serum, or alternatively that this kind of rat serum induces a partial blockade of EP at receptor sites whose activation is a mandatory step for the adequate and full development of cultured erythrocytes.
Gutnisky
A
A
Centro de Estudios Farmacológicos y de Principios Naturales, Universidad de Buenos Aires, Argentina.
Gimeno
A L
AL
D000038:Abscess / Q000097:blood* / Q000209:etiology; D000818:Animals; D001769:Blood; D001854:Bone Marrow Cells; D015672:Erythroid Precursor Cells; D004921:Erythropoietin / Q000494:pharmacology*; D005260:Female; D051381:Rats; D011919:Rats, Inbred Strains; D014425:Turpentine / Q000494:pharmacology*
D004921:Erythropoietin; D014425:Turpentine