Structure of 3'-downstream segment of the human smooth muscle (aortic-type) alpha-actin-encoding gene and isolation of the specific DNA probe.
We isolated the 3'-downstream part of the human aortic smooth muscle alpha-actin (SM alpha A)-encoding gene and determined the nucleotide sequence, including the ninth (last) exon and 3'-untranslated (UT) region. From the comparison of the human 3'-UT region with rat and chicken 3'-UT regions, its homology is lower than those in 3'-UT regions of other actin isoforms such as cardiac alpha-actin and cytoskeletal beta-actin. Therefore, by using the 3'-UT region of the human SM alpha A gene as an actin isoform-specific probe, this gene was detected as a single copy only in the human genome, which expressed the 1.7-kb RNA transcript in an aortic tissue-specific manner.
Kamada
S
S
Department of Oncogene Research, Osaka University, Japan.
Nakano
Y
Y
Kakunaga
T
T
D000199:Actins / Q000096:biosynthesis / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001011:Aorta / Q000648:ultrastructure; D001483:Base Sequence; D002645:Chickens / Q000235:genetics; D003001:Cloning, Molecular; D004247:DNA; D015342:DNA Probes / Q000302:isolation & purification*; D005091:Exons; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D007536:Isomerism; D008969:Molecular Sequence Data; D009130:Muscle, Smooth / Q000648:ultrastructure*; D051381:Rats; D012689:Sequence Homology, Nucleic Acid
D000199:Actins; D015342:DNA Probes; D004247:DNA