title: The emergence and maturation of the first apatite crystals in an in vitro bone formation system.
abstract: Using chick periosteal bone forming cultures we have followed the appearance of the first crystals during de Novo bone formation. The first detectable mineral deposits are crystalline. By x-ray diffraction and dark field imaging we found crystal length to grow from around 100 angstroms on day 4 to (135-160) Angstroms by day 8. The Ca/P ratio rose from 1.46 at day 4 to 1.60 at day 8.
authors.affiliation: Department of Pathology, University of Toronto.
authors.forename: M D
authors.initials: MD
authors.lastname: Grynpas
authors.forename: H C
authors.initials: HC
authors.lastname: Tenenbaum
authors.forename: D P
authors.initials: DP
authors.lastname: Holmyard
mesh_terms: D000818:Animals; D001031:Apatites / Q000378:metabolism*; D001846:Bone Development / Q000502:physiology*; D002642:Chick Embryo; D003460:Crystallization; D046508:Culture Techniques; D014961:X-Ray Diffraction
chemical_list: D001031:Apatites