title: Effect of Staphylococcus aureus serine proteinase on human lymphocyte impairment.
abstract: Assessment of lymphocyte surface membrane integrity by means of trypan exclusion test and longer term survival studies based on 3HTdR uptake and Con A stimulation test were used to estimate effect of Staphylococcus aureus serine proteinase on human lymphocytes in vitro. At concentration of proteinase as high as 10 micrograms/ml, the percentage of trypan-stained lymphocytes increase with enzyme level. In contrast, decrease of 3HTdR level and Con A stimulation is already apparent at concentrations of proteinase ten times lower. Autologous serum exerts strong protective effect. Observed impairment is discussed as significant for local response in inflammation.
authors.affiliation: Department of Animal Biochemistry, Jan Zurzycki Institute of Molecular Biology, Jagellonian University, Kraków, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Turyna
authors.forename: T
authors.initials: T
authors.lastname: Ochałek
mesh_terms: D006801:Humans; D066298:In Vitro Techniques; D007963:Leukocytes, Mononuclear / Q000187:drug effects*; D012697:Serine Endopeptidases / Q000494:pharmacology*
chemical_list: D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase