title: Chromosomal localization and structure of the human P1 protamine gene.
abstract: The human P1 protamine gene and mRNA were amplified with the use of the polymerase chain reaction and cloned into PTZ19R. The sequences were determined which revealed the presence of an intron. Southern and Northern hybridization analyses showed that the gene was single copy and that the mRNA was approximately 450 bases long. The gene was mapped to chromosome 16 with the use of a somatic cell hybrid panel and localized to the 21 region of the q arm by in situ hybridization of the human P1 protamine probe to human metaphase chromosomes.
authors.affiliation: Department of Molecular Biology and Genetics, Wayne State University, Detroit, Michigan 48202.
authors.forename: S A
authors.initials: SA
authors.lastname: Krawetz
authors.forename: M H
authors.initials: MH
authors.lastname: Herfort
authors.forename: J L
authors.initials: JL
authors.lastname: Hamerton
authors.forename: R T
authors.initials: RT
authors.lastname: Pon
authors.forename: G H
authors.initials: GH
authors.lastname: Dixon
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002885:Chromosomes, Human, Pair 16; D006224:Cricetinae; D006801:Humans; D006822:Hybrid Cells; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011479:Protamines / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D011479:Protamines; D012333:RNA, Messenger