Analysis of the membrane-interacting domain of the sea urchin sperm adhesive protein bindin.
We have investigated the domain of the bindin polypeptide that selectively associates with gel-phase phospholipid vesicles. We found that small trypsin fragments of bindin retain the ability to selectively associate with gel-phase vesicles. The primary amino acid sequence of bindin suggests that these peptides are derived from the central portion of the polypeptide between residues 77 and 126, which is the most hydrophobic region of bindin. We have also employed 3-(trifluoromethyl)-3-(m-[125I]iodophenyl)diazirine (TID) and novel, radioiodinated, photoactivatable derivatives of the polar head group of phosphatidylethanolamine (ASD-PE and ASA-PE) to identify membrane-associated polypeptide segments after the transfer of radiolabel from the probe to the bindin polypeptide. After photolysis, bindin was selectively labeled only from probes incorporated in gel-phase vesicles. The labeling of bindin was much more efficient from the head group probes ASA-PE and ASD-PE (8 and 2% of the total label, respectively) in comparison to the hydrophobic probe TID (less than 0.02% of the total label), suggesting that bindin is localized within the polar part of the bilayer. Protease mapping experiments with V8 protease, trypsin, and endoprotease Lys-C suggest that some of the probe label is distributed along the amino-terminal portion of bindin between residues 1 and 76 and the rest of the label is restricted to the segments between residues 77 and 126 which also selectively bind to gel-phase vesicles. The carboxyl-terminal portion of bindin between residues 127 and 236 is not labeled.
Kennedy
L
L
Department of Molecular Biology and Biochemistry, University of California, Irvine 92715.
DeAngelis
P L
PL
Glabe
C G
CG
D000595:Amino Acid Sequence; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D006023:Glycoproteins; D008051:Lipid Bilayers / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000032:analysis*; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D010714:Phosphatidylethanolamines; D010782:Photolysis; D011487:Protein Conformation; D011956:Receptors, Cell Surface; D012617:Sea Urchins; D013094:Spermatozoa / Q000032:analysis*
D006023:Glycoproteins; D008051:Lipid Bilayers; D008565:Membrane Proteins; D010714:Phosphatidylethanolamines; D011956:Receptors, Cell Surface; C035027:egg surface sperm receptor