[Activity of enzymes participating in xenobiotic metabolism and the condition of microsomal membranes of rat liver in vitamin K deficiency].
Alimentary deficiency or vitamin K (vitamin K-poor diet) as well as the vitamin deficiency resulting from sinkumar administration are accompanied by a decreased activity of microsomal demethylases, hydroxylase, NADH- and nNADPH-reductases of dichlorophenolindophenol and neotrazolium. The activity of cytosolic enzymes (only glutathione-S-transferases, aryl- and allyl esterases) is diminished in a lesser degree. Vitamin K deficiency does not significantly interfere with the effect of the xenobiotic metabolism enzyme inducer (phenobarbital) or the cytochrome P-450 inhibitor (cobalt chloride). The changes in the enzyme activity result in a decrease of acetanilide biotransformation. A possible reason for the observed changes in the activity of microsomal enzymes is the weakening of hydrophobic and polar interactions in microsomal membranes. This hypothesis was confirmed by experiments with the use of membrane perturbants as well as by solubilization of membrane-bound enzymes.
Pentiuk
A A
AA
Bogdanov
N G
NG
Khadur
R
R
Lutsiuk
N B
NB
Borisenko
B A
BA
D000083:Acetanilides / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014813:Vitamin K Deficiency / Q000201:enzymology* / Q000378:metabolism; D015262:Xenobiotics / Q000378:metabolism*
D000083:Acetanilides; D015262:Xenobiotics; D003577:Cytochrome P-450 Enzyme System; D009247:NADH, NADPH Oxidoreductases
Aktivnost' fermentov, uchastvuiushchikh v metabolizme ksenobiotikov, i sostoianie mikrosomal'nykh membran pecheni krys pri defitsite vitamina K.