Cloning and expression of ovine placental lactogen.
Ovine placental lactogen (oPL) is active in a wide range of GH and PRL assays, a property that it shares with human GH (hGH). In addition, oPL is one of a small number of hormones that bind the human GH receptor with high affinity. In order to compare the sequence of oPL to the sequences of other members of the GH family, full-length cDNA clones have been isolated. These clones predict that the full sequence of oPL contains 198 amino acids preceded by a 38 amino acid signal sequence. The mature oPL sequence includes six cysteine and two tryptophan residues and shows substantially more identity to bovine PL (67%) and oPL (49%) than to mouse (31%) or human (25%) PL or to oGH (28%) or (26%) hGH. Like the natural hormone, oPL expressed in mammalian tissue cells binds with high affinity to a soluble form of the recombinant hGH receptor. Thus, oPL binds to the human receptor in spite of having a sequence that is considerably divergent from hGH. Interestingly, the sequence of oPL differs from hGH at most of the amino acids recently found by mutagenesis studies to be important residues in the binding of hGH to the human receptor.
Colosi
P
P
Department of Developmental Biology, Genentech, Inc., South San Francisco, California 94080.
Thordarson
G
G
Hellmiss
R
R
Singh
K
K
Forsyth
I A
IA
Gluckman
P
P
Wood
W I
WI
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D015870:Gene Expression; D013006:Growth Hormone; D006801:Humans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010928:Placental Lactogen / Q000235:genetics*; D011986:Receptors, Somatotropin / Q000502:physiology; D012689:Sequence Homology, Nucleic Acid; D012756:Sheep / Q000235:genetics*; D014162:Transfection
D015345:Oligonucleotide Probes; D011986:Receptors, Somatotropin; D013006:Growth Hormone; D010928:Placental Lactogen