Structures of minor ether lipids isolated from the aceticlastic methanogen, Methanothrix concilii GP6.
Structures were determined for two phospholipids and three glycolipids purified from chloroform-methanol extracts of Methanothrix concilii GP6. Together they accounted for 14% of the total lipid and were based on a C20,20-diether core structure consisting of either 2,3-di-O-phytanyl-sn-glycerol or its 3'-hydroxy analog, namely, 2-O-[3,7,11,15-tetramethylhexadecyl]-3-O-[3'- hydroxy-3',7',11',15'-tetramethylhexadecyl]-sn-glycerol. These two core lipids formed phosphodiester bonds to ethanolamine and glycosidic bonds to beta-D-galactopyranose. A third glycolipid consisted of the triglycosyl head group beta-D-galactopyranosyl-(1----6)-[beta-D-glucopyranosyl-(1----3)]-beta-D - galactopyranose in glycosidic linkage to the 3'-hydroxydiether core lipid.
Ferrante
G
G
Division of Biological Sciences, National Research Council of Canada, Ottawa, Ontario.
Brisson
J R
JR
Patel
G B
GB
Ekiel
I
I
Sprott
G D
GD
D055598:Chemical Phenomena; D002621:Chemistry; D002855:Chromatography, Thin Layer; D019605:Euryarchaeota / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D006017:Glycolipids / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010743:Phospholipids / Q000302:isolation & purification*
D006017:Glycolipids; D010743:Phospholipids