DNA-DNA hybridization studies and phenotypic characteristics of strains within the 'Streptococcus milleri group'.
Twenty-five strains resembling 'Streptococcus milleri' were compared by DNA-DNA hybridization, by whole-cell-derived polypeptide patterns on SDS-PAGE, and by biochemical tests. Four homology groups were revealed by DNA-DNA hybridization. DNA homology groups 1, 2 and 3 were closely related and contained the type strains NCDO 2226 (Streptococcus constellatus), NCDO 2227 (Streptococcus intermedius) and NCTC 10713 (Streptococcus anginosus), respectively. DNA homology group 4 consisted of four strains received as variants of Streptococcus intermedius which were found not to be closely related to strains in groups 1-3. The data from SDS-PAGE polypeptide patterns and biochemical tests supported the recognition of three centres of variation within the 'Streptococcus milleri group' corresponding to DNA homology groups 1-3 and indicated that strains of DNA homology group 4 are members of an as yet undescribed species within the viridans streptococci.
Whiley
R A
RA
Department of Oral Microbiology, London Hospital Medical College, UK.
Hardie
J M
JM
D001426:Bacterial Proteins / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D012689:Sequence Homology, Nucleic Acid; D012967:Sodium Dodecyl Sulfate; D013045:Species Specificity; D013291:Streptococcus / Q000032:analysis / Q000145:classification / Q000235:genetics*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012967:Sodium Dodecyl Sulfate