title: Sulfated glycoprotein-1 (saposin precursor) in the reproductive tract of the male rat.
abstract: Sulfated glycoprotein-1 is one of the major protein secretion products of rat Sertoli cells in culture. This 70,000 Mr protein shares substantial sequence similarity with human prosaposin, the precursor of lysosomal saposins. Saposins are known to enhance the activity of lipid modifying enzymes presumably by solubilizing the lipids. We report here the immunolocalization of sulfated glycoprotein-1 in the cells and fluid of the male reproductive tract. The protein is present in secondary lysosomes of Sertoli cells and also in the luminal fluid of seminiferous tubules and epididymis. The highest concentrations of the protein are in seminiferous tubule fluid and rete testis fluid, while relatively low amounts are found in cauda epididymal fluid and serum. Sulfated glycoprotein-1 is believed to be involved in degradation of lipids in residual bodies and may also assist in modification of membrane lipids during sperm maturation.
authors.affiliation: Program in Biochemistry and Biophysics, Washington State University, Pullman 99164.
authors.forename: S R
authors.initials: SR
authors.lastname: Sylvester
authors.forename: C
authors.initials: C
authors.lastname: Morales
authors.forename: R
authors.initials: R
authors.lastname: Oko
authors.forename: M D
authors.initials: MD
authors.lastname: Griswold
mesh_terms: D000818:Animals; D004822:Epididymis / Q000378:metabolism / Q000648:ultrastructure; D006023:Glycoproteins / Q000378:metabolism* / Q000502:physiology; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008854:Microscopy, Electron / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains; D049231:Saposins; D012671:Seminiferous Tubules / Q000378:metabolism / Q000648:ultrastructure; D012708:Sertoli Cells / Q000378:metabolism / Q000648:ultrastructure; D013737:Testis / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D006023:Glycoproteins; C483345:PSAP protein, human; C483347:Psap protein, rat; D049231:Saposins