Radioimmunoassay of neuropeptide Y (NPY) in biological fluids. Circulating NPY levels depend on renal function.
A second antibody solid phase radioimmunoassay for measuring neuropeptide Y (NPY) in biological fluids is introduced. The sensitivity of the method allows also measurements of sub-normal levels of plasma/serum NPY, necessary for obtaining further information of possible biological actions of circulating NPY. Characteristics of the NPY assay procedure are presented together with clinical experiments forced upon a possible renal dependence for NPY. Our data show that NPY at least partly is eliminated by the kidneys. Whether the highly increased NPY levels in patients with renal failure also are involved in the hypertensive mechanisms in these patient groups has to be further evaluated.
Stridsberg
M
M
Department of Clinical Chemistry, University Hospital, Uppsala, Sweden.
Lundqvist
G
G
D000310:Adrenal Gland Neoplasms / Q000097:blood*; D000328:Adult; D001826:Body Fluids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007668:Kidney / Q000503:physiopathology*; D007676:Kidney Failure, Chronic / Q000097:blood*; D008875:Middle Aged; D009478:Neuropeptide Y / Q000032:analysis* / Q000097:blood; D010673:Pheochromocytoma / Q000097:blood*; D011863:Radioimmunoassay; D012016:Reference Values
D009478:Neuropeptide Y