title: Three novel oligosaccharides with the sialyl-Lea structure in human milk: isolation by immunoaffinity chromatography.
abstract: We have determined the structures of three novel oligosaccharides isolated from human milk using the monoclonal antibody MSW 113. These oligosaccharides were purified by affinity chromatography on a column of the immobilized monoclonal antibody and by high-performance liquid chromatography. From the results of 500-MHz 1H NMR spectroscopy and fast atom bombardment-mass spectrometry, their structures were deduced to be (formula; see text) These oligosaccharides bound to MSW 113 to nearly the same extent as sialyl-Lea hexasaccharide but bound to another sialyl-Lea structure-directed monoclonal antibody, NS 19-9, only weakly.
authors.affiliation: Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kitagawa
authors.forename: H
authors.initials: H
authors.lastname: Nakada
authors.forename: A
authors.initials: A
authors.lastname: Kurosaka
authors.forename: N
authors.initials: N
authors.lastname: Hiraiwa
authors.forename: Y
authors.initials: Y
authors.lastname: Numata
authors.forename: S
authors.initials: S
authors.lastname: Fukui
authors.forename: I
authors.initials: I
authors.lastname: Funakoshi
authors.forename: T
authors.initials: T
authors.lastname: Kawasaki
authors.forename: I
authors.initials: I
authors.lastname: Yamashina
authors.forename: I
authors.initials: I
authors.lastname: Shimada
mesh_terms: D002240:Carbohydrate Sequence; D002846:Chromatography, Affinity / Q000379:methods; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008895:Milk, Human / Q000032:analysis*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis*; D014407:Tumor Cells, Cultured
chemical_list: D009844:Oligosaccharides; C016735:sialooligosaccharides