[Influence of several Chinese drugs on the glycogen synthesizing function of primary cultured adult rat hepatocytes].
The influence of several Chinese drugs on the glycogen synthesizing function of normal and carbon tetrachloride-injured hepatocytes was investigated. Hepatocytes prepared from rats fasted for 16 h were incubated with 50 mmol/L glucose. Glycogen content was determined 30 and 90 min after incubation with normal and CCl4-injured cells, respectively. Insulin was used as positive control which increased glycogen content and the data coincided with that in the literature. The following results were obtained (1) Biphenyl-dimethyl-dicarboxylate (BDD) in 100 micrograms/ml concentration increased glycogen content of normal hepatocytes by 88%. It protected cells against CCl4-injury:BDD 10 micrograms/ml remarkably decreased CCl4-induced reduction of glycogen and 100 micrograms/ml showed complete protection. (2) Tremella poly-saccharide slightly increased glycogen content in normal cells, but in a concentration of 100 micrograms/ml it decreased CCl4-induced reduction of glycogen significantly. (3) Low concentration of norcatharidin (10 micrograms/ml) increased glycogen content of normal cells, but at 100 micrograms/ml concentration this effect disappeared. Furthermore, it intensified the toxic effect of CCl4 on glycogen at 10-100 micrograms/ml. (4) CL1500 (100 micrograms/ml) increased glycogen content of normal cells, but it intensified CCl4-injury effect on glycogen at the same concentration.
Wang
H F
HF
Cong
Z
Z
D000818:Animals; D001487:Basidiomycota; D002252:Carbon Tetrachloride Poisoning / Q000378:metabolism; D002478:Cells, Cultured; D004149:Dioxoles / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D006003:Glycogen / Q000096:biosynthesis*; D008099:Liver / Q000166:cytology*; D008314:Malonates / Q000494:pharmacology; D011134:Polysaccharides / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D004149:Dioxoles; D004365:Drugs, Chinese Herbal; D008314:Malonates; D011134:Polysaccharides; C041398:7,7'-dimethoxy-(4,4'-bi-1,3-benzodioxole)-5,5'-dicarboxylic acid dimethyl ester; D006003:Glycogen; C024808:diisopropyl 1,3-dithiol-2-ylidenemalonate