title: Effects of thiopentone on immunoglobulin production in vitro.
abstract: Thiopentone is known to depress several granulocyte and lymphocyte functions. This study assessed the effects of thiopentone on IgG, IgM and IgA production by pokeweed mitogen-activated lymphocytes as a measure of B-lymphocyte function. Thiopentone decreased IgG production at greater than or equal to 25 micrograms ml-1 (91 mumol litre-1) concentrations and that of IgM and IgA at greater than or equal to 50 micrograms ml-1 (182 mumol litre-1) concentrations over 7 days of culture. Preincubation of lymphocytes for 1 h with thiopentone 0-500 micrograms ml-1 (0-1820 mumol litre-1) had no effect on immunoglobulin production, whereas incubation for 2 and 3 h decreased IgG and IgM production with toxic thiopentone concentrations of 500 micrograms ml-1. Hydrocortisone-sensitive suppressor cells and concanavalin A-inducible suppressor cells were more sensitive to high concentrations of thiopentone than the other cell populations. These findings may be important in patients receiving barbiturate treatment for cerebral protection.
authors.affiliation: Department of Anaesthesiology, University Central Hospital, Turku, Finland.
authors.forename: M
authors.initials: M
authors.lastname: Salo
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism; D007075:Immunoglobulin M / Q000378:metabolism; D007136:Immunoglobulins / Q000378:metabolism*; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D008297:Male; D013874:Thiopental / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D013874:Thiopental