title: Effect of oxygen concentration on the formation of malondialdehyde-like material in a model of tissue ischemia and reoxygenation.
abstract: This study was conducted to explore the functional relationship between oxygen concentration during tissue reoxygenation after ischemia and the extent of postischemic lipid peroxidation, an indicator of reoxygenation injury. Excised rat liver or kidney tissue was rendered ischemic for 1 h at 37 degrees C, minced into 1 mm3 fragments, and then reoxygenated for 1 h in flasks of buffered salt solution containing various amounts of oxygen. Production of malondialdehyde-like material (MDA) was measured to indicate lipid peroxidation. MDA production was minimal at oxygen tensions less than 10 mmHg, increased sharply from 10 to 50 mmHg, and plateaued at approximately 100 mmHg. A similar functional relationship was produced by a simple mathematical model of free radical mediated lipid peroxidation in biological membranes, suggesting that MDA production is indeed caused by free radical oxidation of membrane phospholipids and that the oxygen effect is governed by simple competition between chain propagation and chain termination reactions within the membrane. These experimental and analytical results confirm that relatively low concentrations of oxygen are sufficient to produce oxidative damage in post-ischemic tissues.
authors.affiliation: Hillenbrand Biomedical Engineering Center, Purdue University, West Lafayette, IN 47907.
authors.forename: S C
authors.initials: SC
authors.lastname: Salaris
authors.forename: C F
authors.initials: CF
authors.lastname: Babbs
mesh_terms: D000818:Animals; D005609:Free Radicals; D007511:Ischemia / Q000378:metabolism*; D007668:Kidney / Q000098:blood supply*; D015227:Lipid Peroxidation; D008099:Liver / Q000098:blood supply*; D008297:Male; D008314:Malonates / Q000378:metabolism*; D008315:Malondialdehyde / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D015427:Reperfusion Injury / Q000378:metabolism; D013858:Thiobarbiturates / Q000494:pharmacology
chemical_list: D005609:Free Radicals; D008314:Malonates; D013858:Thiobarbiturates; D008315:Malondialdehyde; D010100:Oxygen