Reversed phase high performance liquid chromatography of glycopeptides.
A practical procedure for isolating and purifying glycopeptides is described, viz. enzymatic hydrolysis-gel permeation chromatography-ion exchange chromatography-reversed phase HPLC. Using this procedure 28 glycopeptides from hen ovalbumin have been isolated some of which hitherto have not been identified. Water was a suitable mobile phase for preparing pure glycopeptides, and control of column temperature was important for good separations and reproducible retention times. Structural confirmation was by fast atom bombardment mass spectrometry.
Wang
T H
TH
Department of Agricultural Chemistry, Oregon State University, Corvallis 97331.
Chen
T
T
Barofsky
D F
DF
D002851:Chromatography, High Pressure Liquid; D006020:Glycopeptides / Q000302:isolation & purification*; D008970:Molecular Weight; D010047:Ovalbumin / Q000302:isolation & purification*; D010059:Ovomucin / Q000302:isolation & purification; D013696:Temperature
D006020:Glycopeptides; D010059:Ovomucin; D010047:Ovalbumin