New approach to eliminate HLA class I antigens from platelet surface without cell damage: acid treatment at pH 3.0.
A new method was studied for eliminating HLA class I antigens from the surface of platelets without damaging the cells. Platelets were exposed to an acid solution (pH 3.0) to eliminate the antigenicity of HLA class I antigens. The reduction in antigenicities of HLA class I common antigen and individual HLA class I antigens by acid treatment was marked. Patients' sera which contained multispecific HLA antibodies reacted with PBS-treated platelets, but not with acid-treated platelets. No changes were observed in the antigenicities of glycoprotein Ib or glycoprotein IIb/IIIa. The viability of acid-treated platelets was 83%. Ultrastructural investigations revealed no significant difference between the PBS-treated platelets and acid-treated platelets. The platelet function studies showed that the aggregation of acid-treated platelets induced by various agonists was only slightly reduced compared with PBS-treated platelets. We propose that acid-treated platelets are promising for clinical use in patients refractory to platelet transfusions and may be superior to chloroquine-treated platelets for analysis of the specificity of antiplatelet antibodies.
Kurata
Y
Y
Department of Blood Transfusion, Osaka University Hospital, Japan.
Oshida
M
M
Take
H
H
Furubayashi
T
T
Nakao
H
H
Tomiyama
Y
Y
Kanayama
Y
Y
Nagao
N
N
Okubo
Y
Y
Yonezawa
T
T
D001792:Blood Platelets / Q000032:analysis / Q000187:drug effects* / Q000276:immunology; D002470:Cell Survival / Q000187:drug effects; D055598:Chemical Phenomena; D002621:Chemistry; D002951:Citrates / Q000494:pharmacology*; D019343:Citric Acid; D006680:HLA Antigens / Q000032:analysis / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000032:analysis / Q000276:immunology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008562:Membrane Glycoproteins / Q000032:analysis / Q000276:immunology
D002951:Citrates; D006680:HLA Antigens; D015395:Histocompatibility Antigens Class I; D008562:Membrane Glycoproteins; D019343:Citric Acid