Isolation of medically significant Vibrio species from riverine sources in south east Queensland.
Vibrio and Vibrio-like bacteria were isolated from water, sediments, plants and faeces from eight riverine sites in South East Queensland, Australia, using filtrations, enrichments and selective growth on thiosulphate-citrate-bile salts-sucrose (TCBS) and Simidu agars. Isolates (402 in toto) were classified by numerical analysis of phenotypic characteristics and comparison with fifteen reference cultures. Of the isolates 41 (10.2%) were identified as Vibrio cholerae, 33 (8.2%) as V. fluvialis and 118 (29.4%) as motile Aeromonas species. Other isolates resembled V. parahaemolyticus, V. alginolyticus, V. vulnificus and V. hollisae. No isolates were positively identified as V. damsela or Plesiomonas shigelloides. Vibrio species tolerant of low salt levels and aeromonads were widely distributed in riverine locations but more halophilic species were restricted to more saline areas. Simidu agar failed to select for vibrios as effectively as TCBS agar. Enrichment for 18 h produced more isolates than for 6 h.
Myatt
D C
DC
Department of Microbiology, University of Queensland, St Lucia, Brisbane, Australia.
Davis
G H
GH
D003470:Culture Media; D005618:Fresh Water; D011793:Queensland; D014733:Vibrio / Q000145:classification / Q000302:isolation & purification*; D014871:Water Microbiology
D003470:Culture Media