Protein characterization of sexually mature and immature forms of Schistosoma mansoni.
1. Protein composition of different stages of Schistosoma mansoni was compared using specific antisera, 2D polyacrylamide gel electrophoresis and 14-C-leucine incorporation into proteins. 2. Major qualitative differences were detected when an anti-membrane antiserum was used. 3. 2D gel electrophoresis showed that the protein composition varied when mature and immature females were compared, whereas no differences were noted when mature and immature male worms were compared. 4. Experiments measuring protein synthesis by the different schistosome stages confirmed that upon maturation, only the female schistosomes displayed qualitative differences. 5. The protein pattern of the male schistosomes did not vary significantly as a function of development.
Braga
V M
VM
Departamento de Bioquimica/Imunologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Brazil.
Tavares
C A
CA
Rumjanek
F D
FD
D000818:Animals; D015153:Blotting, Western; D002250:Carbon Radioisotopes; D005591:Chemical Fractionation; D015180:Electrophoresis, Gel, Two-Dimensional; D015801:Helminth Proteins / Q000032:analysis*; D007930:Leucine / Q000378:metabolism; D012550:Schistosoma mansoni / Q000502:physiology*; D012727:Sex Characteristics; D012741:Sexual Maturation / Q000502:physiology*
D002250:Carbon Radioisotopes; D015801:Helminth Proteins; D007930:Leucine