title: Effect of an acute maternal fluoride dose on fetal plasma fluoride levels and enamel fluoride uptake in guinea pigs.
abstract: We conducted this study to measure maternal plasma, fetal plasma, and fetal enamel fluoride concentrations for four hours following an oral F dose to near-term pregnant guinea pigs. We placed female guinea pigs on de-ionized (Group I) or 3-ppm-F (Group II) drinking water prior to breeding and during gestation. On the 57th day of gestation, we administered a maternal dose of NaF solution (0.6 mg F/kg) by stomach tube. We collected samples of maternal plasma, fetal plasma, and fetal enamel at baseline, at 15 and 30 min, and at one, two, and four h after administration of the dose. We assayed samples for F using a modification of the micro-diffusion and ion-specific electrode method. Group I mean baseline F values were: maternal plasma, 0.016; fetal plasma, 0.002; and fetal enamel, 7.0 ppm. Group II mean values were: 0.055, 0.004, and 19.0 ppm. After the maternal fluoride dose, the mean maternal plasma [F] rose sharply for 30 to 60 min and declined to about 50% of peak values by four h. Fetal plasma [F] changed less in absolute values, but similarly to maternal changes relative to baseline. Fetal enamel mean [F] rose more in Group II than in Group I. Baseline F status had an important effect on F uptake in fetal enamel following an acute maternal fluoride dose.
authors.affiliation: Department of Pediatric Dentistry, University of North Carolina, School of Dentistry, Chapel Hill 27599.
authors.forename: J W
authors.initials: JW
authors.lastname: Bawden
authors.forename: T G
authors.initials: TG
authors.lastname: Deaton
authors.forename: G G
authors.initials: GG
authors.lastname: Koch
authors.forename: B P
authors.initials: BP
authors.lastname: Crawford
mesh_terms: D000284:Administration, Oral; D000818:Animals; D003743:Dental Enamel / Q000032:analysis*; D005260:Female; D005312:Fetal Blood / Q000032:analysis*; D005459:Fluorides / Q000008:administration & dosage / Q000032:analysis / Q000097:blood*; D006168:Guinea Pigs; D008431:Maternal-Fetal Exchange; D011247:Pregnancy; D013997:Time Factors
chemical_list: D005459:Fluorides