Epidermal growth factor receptors in rat placenta, amnion and yolk sac: characteristics of specific binding are dependent on gestational age.
Studies of [125I]-EGF binding to the rat placenta, amnion and yolk sac were carried out on days 14, 17 and 20 of gestation. In the placenta EGF binding was detectable on all 3 days; in the amnion EGF binding was undetectably low on day 14 but was present on days 17 and 20, while in the yolk sac EGF binding was undetectably low on all 3 days. Although Scatchard analysis of EGF binding to placental tissue raised the possibility of high and low affinity receptors, a statistical analysis of the ligand binding data was consistent with the presence of only one type of EGF receptor. The overall affinity of the receptors did not change with stage of gestation. However, the concentration of EGF receptors was lower in placental tissue on day 17 than on days 14 or 20 of gestation; the receptor concentrations were similar on days 14 and 20. It is suggested that EGF binding to the placenta, amnion, and yolk sac may reflect the levels of cell proliferation in those tissues in the latter part of gestation.
Ali
P
P
Department of Child Health, University of Manchester, UK.
Smart
J L
JL
D'Souza
S W
SW
D000650:Amnion / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D001667:Binding, Competitive; D066246:ErbB Receptors / Q000032:analysis* / Q000378:metabolism; D005260:Female; D005865:Gestational Age; D010920:Placenta / Q000032:analysis / Q000378:metabolism*; D011247:Pregnancy; D051381:Rats; D015017:Yolk Sac / Q000032:analysis / Q000378:metabolism*
D066246:ErbB Receptors