[Luminol-amplified chemiluminescence evaluation of the microbicidal property of alveolar macrophages in response to drugs].
The present study was designed to evaluate the validity of luminol-dependent chemiluminescence (CL) to evaluate the microbicidal properties of alveolar macrophages (AM) in response to drugs. It was observed that production of CL is associated with H2O2 as well as O2-, 1O2, .OH. Furthermore, our data revealed that CL is dependent not only upon the extracellular release of these oxygen radicals, but also upon intracellular metabolic events, which thus allows a complete analytical study in any given state. It should also be noted from our data that the effect of antibacterial agents on CL was greatly affected by the source of the target cells. The defect of amplified CL in AM obtained from young rabbits might be attributable to immaturity of AM.
Chida
K
K
Sato
A
A
Okano
A
A
Gemma
H
H
Iwata
M
M
Yasuda
K
K
Shichi
I
I
Shirai
M
M
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D008163:Luminescent Measurements; D008165:Luminol; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D011650:Pulmonary Alveoli / Q000166:cytology*; D011724:Pyridazines; D011817:Rabbits
D000900:Anti-Bacterial Agents; D011724:Pyridazines; D008165:Luminol