[The effect of liposomes containing cholesterol or 7-ketocholesterol on the capacity of cultured cells to form colonies].
The experiments with the cultivated Chinese hamster cells showed that the incubation of cells with (phosphatidylcholine+cholesterol+7-keto-cholesterol)--containing liposomes (4:3:1 by weight) during two hours leads to a decrease in the effectiveness of colony-forming up to zero, while the (phosphatidylcholine+cholesterol-containing liposomes (1:1 by weight) reduce the effectiveness by 90%. Furthermore, the cholesterol-containing liposomes cause the decrease of the number of colonies with maximal size, accompanied by the increase of the number of colonies with middle size.
Khokhlov
A N
AN
Prokhorov Liu
Zakharova
T S
TS
Ivanova
A S
AS
Khalilov
E M
EM
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000494:pharmacology*; D003114:Colony-Forming Units Assay; D006224:Cricetinae; D003412:Cricetulus; D004337:Drug Carriers; D007653:Ketocholesterols / Q000494:pharmacology*; D008081:Liposomes; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects*
D004337:Drug Carriers; D007653:Ketocholesterols; D008081:Liposomes; D002784:Cholesterol; C003001:7-ketocholesterol
Vliianie liposom, soderzhashchikh kholesterin ili 7-ketokholesterin, na sposobnost' kul'tiviruemykh kletok k obrazovaniiu koloniĭ.