Medulloblastoma cell line secretes platelet-derived growth factor.
Medulloblastoma is the most common malignant childhood brain tumor in which aggressive growth produces recurrence in approximately 50% of appropriately treated cases and metastases along the neuraxis in 30%. To date, no studies exist concerning the production of autocrine growth factors by this brain tumor type. Malignant brain tumors in adults often produce platelet-derived growth factor (PDGF). A medulloblastoma cell line, TE-671, has been used for many years in pediatric neuro-oncologic studies. We assayed this medulloblastoma cell line for the production of PDGF. PDGF is produced by medulloblastoma cells grown in monolayer tissue culture and stimulates PDGF-sensitive 3T3 fibroblasts to incorporate tritiated thymidine in a dose-dependent fashion. This biologic activity is blocked by PDGF antibodies in a dose-dependent relationship. We postulate that PDGF produced by medulloblastoma cells plays a role in the growth of this tumor by stimulating mitogenic activity.
Whelan
H T
HT
Department of Neurology, Medical College of Wisconsin, Milwaukee 53226.
Nelson
D B
DB
Strother
D
D
Przybylski
C
C
Figge
G
G
Mamandi
A
A
D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D008527:Medulloblastoma; D010982:Platelet-Derived Growth Factor / Q000276:immunology / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism*
D007106:Immune Sera; D010982:Platelet-Derived Growth Factor