Genetic and mutational tools for investigating the genetics and molecular biology of trichothecene production in Gibberella pulicaris (Fusarium sambucinum).
Gibberella pulicaris (Fusarium sambucinum) is a promising organism for studying the genetics and regulation of trichothecene biosynthesis; conditions for obtaining fertile crosses have been defined (Desjardins & Beremand, 1987) and crosses between natural variants have provided some information about the number, location, arrangement, and role of genes which determine trichothecene production (Desjardins & Beremand, 1987; Beremand & Desjardins, 1988). The development of some additional experimental tools and methodologies required for the further genetic analysis of trichothecene production in G. pulicaris are described in the present study. A highly fertile, isogenic line was constructed for G. pulicaris strain R-6380. The ability to readily generate mutants in this strain was also demonstrated. Both biochemical and morphological mutants were obtained following UV-mutagenesis. The inheritance of some of these mutations through meiosis indicated that they will be useful genetic markers for crosses and mapping studies. Since strain R-6380 is also transformable (Salch & Beremand, 1988), it is an excellent choice for transmission and molecular genetic studies involving trichothecene production.
Beremand
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MN
U.S. Department of Agriculture, Agricultural Research Service, Northern Regional Research Center, Peoria, IL 61604.
D003433:Crosses, Genetic; D005260:Female; D005670:Fusarium / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D005874:Gibberella / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D006999:Hypocreales / Q000378:metabolism*; D008297:Male; D008540:Meiosis; D009154:Mutation; D012717:Sesquiterpenes; D014255:Trichothecenes / Q000096:biosynthesis / Q000235:genetics*; D014466:Ultraviolet Rays
D012717:Sesquiterpenes; D014255:Trichothecenes