[Flow cytometric bromodeoxyuridine (BrdU)/DNA analysis--study with urogenital tumors].
We performed the simultaneous flow cytometric bromodeoxyuridine (BrdU)/DNA analysis in combination with in vitro BrdU labeling method using seven human urogenital tumors. The DNA content and incorporated BrdU content of each tumor cell was analyzed quantitatively using this flow cytometric method. The cell kinetics of each cell line of heterogeneous tumor could be analyzed by this combined method. In the near future, by establishing a procedure decreasing non-specific staining of cells, the development of this flow cytometric two-color analysis in clinical fields is expected.
Shimabukuro
T
T
Department of Urology, Yamaguchi University School of Medicine.
Yoshihiro
S
S
Matsuyama
H
H
Yamamoto
N
N
Sakatoku
J
J
D000368:Aged; D001973:Bromodeoxyuridine; D002295:Carcinoma, Transitional Cell / Q000032:analysis* / Q000473:pathology; D002453:Cell Cycle; D002648:Child; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D014407:Tumor Cells, Cultured; D014565:Urogenital Neoplasms / Q000032:analysis* / Q000473:pathology
D004273:DNA, Neoplasm; D001973:Bromodeoxyuridine