Centrifugal counter-current partition chromatography with helical coil rotor. Simplified counter-current chromatography with a rotating face-seal.
A centrifugal counter-current partition chromatograph has been developed and tested in order to simplify earlier counter-current chromatographic (CCC) procedures. It includes a helical coil rotor and a rotating face-seal. The rotor is designed to be adapted in an ordinary laboratory centrifuge for toroidal coil CCC. Twisting between the inlet and outlet tubing is avoided by using the rotating seal in the rotor. The seal is placed between the rotor, on which helical coils are mounted, and a newly designed centrifuge lid. This chromatographic rotor, rotating simply around its own axis, has simplified a previous CCC device in which a coil planet mechanism is used to avoid tube twisting whilst retaining the capability for chromatographic separations. Results for separations of nystatin, dinitrophenylamino acids and Poly I:C were comparable to those obtained by liquid chromatography and the previous CCC procedure.
Kim
L S
LS
Instrumentation Department, Institute of Biophysics, Academia Sinica, Beijing, China.
Tian
Z Q
ZQ
Li
X N
XN
Zhang
Y
Y
Wang
J
J
D000596:Amino Acids / Q000032:analysis; D002845:Chromatography / Q000295:instrumentation*; D004136:Dinitrobenzenes / Q000032:analysis; D009761:Nystatin / Q000032:analysis; D011070:Poly I-C / Q000032:analysis
D000596:Amino Acids; D004136:Dinitrobenzenes; D009761:Nystatin; D011070:Poly I-C