[Effect of sera from children with acute lymphoblastic leukemia on the morphology of granulocyte-macrophage colonies grown from murine bone marrow cells and the cells of murine myelomonocytic leukemia WEHI 3B D+].
Active role of the T lymphocytes in the regulation process of the granulo- and monocytes colonies formation has been recently emphasized. The cells are a source of number of factors stimulating proliferation and differentiation of both, the steered stem cells and multipotential cells. The possibility of the liberation by some classes of T lymphocytes of substances with inhibitory faculties is also recently suggested. Such vast function of these cells in the process of granulo- and monocyte formation in normal conditions brings forward the question of their role in the proliferating diseases of the lymphatic system especially in connection with some research indicating the presence of changes of the regulators in the course of proliferating diseases of the bone marrow. Taking into account above data investigations have been carried on aimed at the demonstration to what degree stimulators or inhibitors which can be present in the blood serum of children with acute lymphoblastic leukemia are able to determine the composition and morphology of colonies arising from stem cells of bone marrow and of the cells of myelomonocytic leukemia of WEHI 3B,D+ line. This creates the possibility of determination of the nature of the examined substances and of the effect to the treatment on their concentration. The activity of the regulators has been examined in the semi-liquid agar culture "in vitro" applying the Bredley and Metcalf method. The effect of the treatment applied on the investigated parameters has been also assessed. In children with acute lymphoblastic leukemia prior to the initiation of treatment, the increased activity distinctly stimulating the growth of granulocytic and only slightly of macrophage colonies has been present.(ABSTRACT TRUNCATED AT 250 WORDS)
Swiderska
J
J
Szmitkowski
M
M
Kondracka
E E
EE
D000818:Animals; D001853:Bone Marrow / Q000473:pathology*; D002648:Child; D003114:Colony-Forming Units Assay; D006098:Granulocytes / Q000473:pathology*; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D006801:Humans; D066298:In Vitro Techniques; D015477:Leukemia, Myelomonocytic, Chronic / Q000473:pathology*; D008264:Macrophages / Q000473:pathology*; D051379:Mice; D009374:Neoplasms, Experimental / Q000473:pathology; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000097:blood*; D014410:Tumor Stem Cell Assay
Wpływ surowic dzieci w przebiegu ostrej białaczki limfoblastycznej na morfologie kolonii granulocytarno-makrofagowych (GM) powstałych z komórek macierzystych szpiku myszy (CFC) oraz komórek mysiej białaczki mielomonocytarnej WEHI 3B D+.