Purification and some properties of thiosulphate-cleaving enzyme from Thiobacillus novellus.
A thiosulphate-cleaving enzyme was purified from Thiobacillus novellus and some of its properties studied. The enzyme showed an absorption peak at 279 nm and no peaks between 300 and 650 nm. Its Mr was 38,000. Although the crude enzyme cleaved thiosulphate to form sulphite without addition of cyanide, the purified enzyme required cyanide to cleave thiosulphate. The Km values for thiosulphate and cyanide of the purified enzyme were 1.0 mM and 0.3 mM, respectively. One mol of the enzyme formed 10 mol of thiocyanate per s from thiosulphate and cyanide. The thiosulphate-cleaving activity of the enzyme was strongly inhibited by cysteine, while beta-mercaptoethanol was less inhibitory. The factor which accepted sulphur from thiosulphate in the crude preparation of thiosulphate-cleaving enzyme seemed to be a relatively labile compound with an Mr of 10,000 x 20,000.
Fukumori
Y
Y
Department of Life Science, Faculty of Science, Tokyo Institute of Technology, Japan.
Hoshiko
K
K
Yamanaka
T
T
D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D003486:Cyanides / Q000494:pharmacology; D003545:Cysteine / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D008623:Mercaptoethanol / Q000494:pharmacology; D008970:Molecular Weight; D013466:Sulfurtransferases / Q000378:metabolism*; D013855:Thiobacillus / Q000201:enzymology*; D013884:Thiosulfate Sulfurtransferase / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D013885:Thiosulfates / Q000378:metabolism*
D003486:Cyanides; D013885:Thiosulfates; D008623:Mercaptoethanol; D013466:Sulfurtransferases; D013884:Thiosulfate Sulfurtransferase; D003545:Cysteine