title: A method for detection of cellulases in polyacrylamide gels using 5-bromoindoxyl-beta-D-cellobioside: high sensitivity and resolution.
abstract: The assay of endo-1,4-beta-glucanases (cellulases) from Trichoderma reesei, T. longibrachiatum, and Sporotrichum pulverulentum by 5-bromoindoxyl-beta-D-cellobioside is described. The substrate is enzymatically cleaved to afford 5-bromoindoxyl and latter undergoes immediate azo coupling with Fast Red or oxidation by nitroblue monotetrazolium chloride, various forms of endoglucanases which can thus be assayed in polyacrylamide gel.
authors.affiliation: Department of Chemistry, Moscow State University, USSR.
authors.forename: V M
authors.initials: VM
authors.lastname: Chernoglazov
authors.forename: O V
authors.initials: OV
authors.lastname: Ermolova
authors.forename: Y V
authors.initials: YV
authors.lastname: Vozny
authors.forename: A A
authors.initials: AA
authors.lastname: Klyosov
mesh_terms: D000180:Acrylic Resins / Q000032:analysis*; D002475:Cellobiose / Q000031:analogs & derivatives; D002480:Cellulase / Q000032:analysis* / Q000378:metabolism; D004187:Disaccharides; D004591:Electrophoresis, Polyacrylamide Gel; D005069:Evaluation Studies as Topic; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000032:analysis
chemical_list: D000180:Acrylic Resins; D004187:Disaccharides; D007527:Isoenzymes; C016680:polyacrylamide gels; C062427:5-bromoindoxyl-beta-cellobioside; D002475:Cellobiose; D002480:Cellulase