title: [Changes in biological properties of tick-borne encephalitis virus during cleavage of disulfide bonds in protein E].
abstract: The study showed the disruption of disulphide bonds in E protein of tick-borne encephalitis virus (TBE) to lead to the loss of antigenicity, infectivity, hemagglutinating and protective activities. The loss of infectivity under the effect of a thiolic reagent appears to be associated with block of the very first stage of virus-cell interaction, virus adsorption on the target cell. An attempt to reestablish the E protein structure and the above-mentioned virus properties after the removal of the thiolic reagent failed. The role of tertiary structure of E protein in the manifestation of TBE virus main biological properties is discussed.
authors.forename: M F
authors.initials: MF
authors.lastname: Vorovich
authors.forename: A V
authors.initials: AV
authors.lastname: Timofeev
authors.forename: D G
authors.initials: DG
authors.lastname: Mal'dov
authors.forename: V I
authors.initials: VI
authors.lastname: Khaustov
authors.forename: L B
authors.initials: LB
authors.lastname: El'bert
mesh_terms: D000818:Animals; D004220:Disulfides / Q000032:analysis*; D004229:Dithiothreitol; D004669:Encephalitis Viruses, Tick-Borne / Q000032:analysis* / Q000648:ultrastructure; D051379:Mice; D008854:Microscopy, Electron; D011487:Protein Conformation; D014759:Viral Envelope Proteins / Q000032:analysis*
chemical_list: D004220:Disulfides; D014759:Viral Envelope Proteins; D004229:Dithiothreitol
vernacular_title: Izmeneniia biologicheskikh svoĭstv virusa kleshchevogo éntsefalita pri razryve disul'fidnykh sviazeĭ v belke E.