title: Nucleotide sequence of SN-07 chromophore binding site.
abstract: DNase I footprinting was used to investigate binding sites for SN-07 chromophore on DNA fragments prepared from pBR322. Six sites were protected on about 150 base pair DNA fragments by SN-07 chromophore, but not by related anthracycline antibiotics from DNase I digestion. All the protected sites contained the dinucleotide sequence 5'-GC-3', but no other regularities could be discerned. A drug-induced conformational change of DNA was suggested by enhancement of DNase I sensitivity between the protected sites. These results support covalent interaction of the carbinolamine function of SN-07 chromophore to 2-amino group of guanine residues.
authors.affiliation: Research Institute of Life Science, Snow Brand Milk Products Co., Ltd., Tochigi, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kimura
authors.forename: T
authors.initials: T
authors.lastname: Morinaga
authors.forename: N
authors.initials: N
authors.lastname: Miyata
authors.forename: G
authors.initials: G
authors.lastname: Kawanishi
mesh_terms: D018943:Anthracyclines; D000903:Antibiotics, Antineoplastic / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000378:metabolism*; D003850:Deoxyribonuclease I; D004591:Electrophoresis, Polyacrylamide Gel
chemical_list: D018943:Anthracyclines; D000903:Antibiotics, Antineoplastic; C047795:SN-07; D004247:DNA; D003850:Deoxyribonuclease I