A thyroid hormone-vasopressin interaction promotes survival and maturation of hippocampal neurons dissociated postnatally.
Hippocampal cells dissociated from 5-day-old rat pups were grown in a suitable chemically defined basal medium, supplemented or not with 3,3',5-triiodo-L-thyronine (T3), [Arg8]-vasopressin (AVP), or both, at different concentrations. Four days after plating, neuron-like cells began to degenerate drastically in the basal medium. Although AVP alone had no effect, T3, and to a greater extent T3 and AVP together, prevented their death. Moreover, T3 and AVP also acted synergically in promoting the maturation of surviving cells, especially AchE-positive neurons, either directly or through glial cells.
Clos
J
J
Unité de Neurobiologie Endocrinologique, CNRS, URA 1197, Université de Montpellier II, France.
Gabrion
J
J
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006624:Hippocampus / Q000166:cytology* / Q000187:drug effects; D051381:Rats; D014284:Triiodothyronine / Q000494:pharmacology*; D014667:Vasopressins / Q000494:pharmacology*
D014284:Triiodothyronine; D014667:Vasopressins