[Hormonal regulation of GCDFP-15 secretion in breast tumors. Study of cultured cells].
We studied the hormonal regulation of the Gross Cystic Disease Fluid Protein 15 kd (GCDFP-15) which is secreted by mammary epithelial cells. Its precise role is still unknown. For some authors, it is associated with a risk of breast cancer, and for others with a functional differentiation of the cells. We measured the secretion of GCDFP-15 by an immunoenzymatic method in culture media and cytosols of mammary epithelial cells MCF-7, MDA-MB-231 and HBL-100. We also measured the GCDFP-15 secreted by explants of human tumors in organ cultures. We compared the basal values of GCDFP-15 with those obtained after estradiol, dihydrotestosterone and tamoxifen stimulations. Our results show a decrease of GCDFP-15 production with estradiol and an increase with dihydrotestosterone and tamoxifen. These variations are not systematically correlated with variations of cell growth. This study highlights the interest of GCDFP-15 as a tumoral marker; the study of GCDFP-15 level variations could be useful in the monitoring of cancer response to hormonal and/or chemotherapeutic treatment.
Boutteville
C
C
Laboratoire d'endocrinologie expérimentale, centre Oscar-Lambret, Lille, France.
Revillion
F
F
Vandewalle
B
B
Lefèbvre
J
J
D001053:Apolipoproteins; D053399:Apolipoproteins D; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D002352:Carrier Proteins; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003864:Depression, Chemical; D013196:Dihydrotestosterone / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006023:Glycoproteins; D006801:Humans; D026901:Membrane Transport Proteins; D009363:Neoplasm Proteins / Q000378:metabolism*; D009924:Organ Culture Techniques; D013268:Stimulation, Chemical; D013629:Tamoxifen / Q000494:pharmacology*
C024328:APOD protein, human; D001053:Apolipoproteins; D053399:Apolipoproteins D; D002352:Carrier Proteins; D006023:Glycoproteins; D026901:Membrane Transport Proteins; D009363:Neoplasm Proteins; C488892:PIP protein, human; D013196:Dihydrotestosterone; D013629:Tamoxifen; D004958:Estradiol
Régulation hormonale de la sécrétion de GCDFP-15 dans les tumeurs mammaires. Etude en culture de cellules.