Synthesis and properties of RNA with catalytic activity.
Ribooligonucleotides, which form the self-cleavage domain of a satellite RNA of tobacco ringspot virus, were synthesized by the solid-phase phosphoramidite method using o-nitrobenzyl groups for 2'-hydroxyl protection. The oligomers were obtained in quantities sufficient for NMR measurement. Specific cleavage at an expected site was observed when the three RNA fragments were mixed in the presence of magnesium ions.
Odai
O
O
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Sakata
T
T
Orita
M
M
Hiroaki
H
H
Uesugi
S
S
Tanaka
T
T
D001483:Base Sequence; D002384:Catalysis; D008274:Magnesium; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000138:chemical synthesis* / Q000378:metabolism
D009843:Oligoribonucleotides; D008274:Magnesium