Reconstruction of parodontal tissue with chitosan.
Chitosan ascorbate, obtained by mixing chitosan with ascorbic acid and sodium ascorbate, was produced in a gel form suitable for the treatment of periodontitis according to current dental surgery. While chitosan ascorbate underwent degradation in vitro, especially in the presence of atmospheric oxygen and at pH 6.0, the protection from oxygen offered by the surgical cements and the physiological pH value permitted chitosan ascorbate to play an important biological role in vivo, where it kept a honeycomb structure, as indicated by SEM on biopsies taken on 10 patients. The proliferation and organization of the cells were thus favoured with a subsequent enhanced capability of reconstructing a histoarchitectural tissue. Chitosan was progressively reabsorbed by the host, with very satisfactory clinical recoveries of the 52 defects treated, for which tooth mobility and pocket depths were significantly reduced.
Muzzarelli
R
R
Faculty of Medicine, University of Ancona, Italy.
Biagini
G
G
Pugnaloni
A
A
Filippini
O
O
Baldassarre
V
V
Castaldini
C
C
Rizzoli
C
C
D000328:Adult; D001205:Ascorbic Acid; D001672:Biocompatible Materials; D002686:Chitin / Q000031:analogs & derivatives*; D048271:Chitosan; D005260:Female; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D010518:Periodontitis / Q000601:surgery*; D010519:Periodontium / Q000601:surgery* / Q000648:ultrastructure
D001672:Biocompatible Materials; D002686:Chitin; D048271:Chitosan; D001205:Ascorbic Acid