[Assessment of cardiac rejection following heart and lung transplantation (II)].
To investigate the histologic consequence of combined heart and lung transplantation, 15 transplanted hearts were obtained from heterotopic heart and lung transplantation using rat model. The allografts were randomly obtained between 1 and 13 days after the transplantation without use of any immunosuppressants. Based upon our criteria established in our previous study, the degree of cardiac rejection was evaluated using rejection score, independently at seven segments in both ventricles, which are 1) Right Ventricle Free Wall (RVFW), 2) Transient zone between RVFW and Septum (RVFW-SEP), 3) Septum (RVSEP), 4) Left Ventricle (Right outer layer) (LVRO), 5) Left Ventricle (Left outer layer) (LVLO), 6) Left Ventricle (Right inner layer) (LVRI), 7) Left Ventricle (Left inner layer)(LVLI). The degree of cardiac rejection in each segment shows differently and the rejection initiates from RVFW and spreads into RVFW-SEP and outer layers of left ventricle. The segments of RVSEP and inner layers of left ventricle remained normal longer than other segments, and were thought to be less sensitive to diagnose the rejection in early phase. Significant correlation between post-transplanted days and the degree of rejection was observed in all segments except RVSEP. In conclusion, this study demonstrates that the myocardial biopsy obtained from RVFW, RUVF-SEP and LVRO after the combined heart and lung transplantation might be more sensitive than any other segments including RVSEP.
Hachida
M
M
Kurosawa
H
H
Koide
M
M
Koyanagi
H
H
D000818:Animals; D006084:Graft Rejection; D016041:Heart-Lung Transplantation / Q000473:pathology / Q000502:physiology; D008168:Lung / Q000473:pathology; D008297:Male; D009206:Myocardium / Q000473:pathology; D051381:Rats; D011915:Rats, Inbred BUF; D011917:Rats, Inbred Lew; D016042:Transplantation, Heterotopic / Q000473:pathology / Q000502:physiology