Effects of in utero exposure to cyclophosphamide in mice. II. Assessment of immunocompetence of offspring from 5 to 10 weeks of age.
Offspring of mice treated with cyclophosphamide (Cy; 1, 2.5 or 5 mg/kg) during pregnancy (6-18 days of gestation) and tested for immunocompetence from 5 to 10 weeks of age were found to have defective reticuloendothelial clearance. The main effects were: a) increased elimination half time (T 1/2) of 51Cr-labeled SRBC from circulation, b) decreased liver uptake of 51Cr and c) impaired ability of the spleen, mostly affecting the female pups, to compensate for decreased liver uptake. The highest dose group suffered the most pronounced effects. This group was also found to have increased IgG immunoglobulin levels at 7 weeks of age. IgG antibody production in response to specific antigenic stimulation and delayed hypersensitivity reactions to oxazolone did not appear to be affected by Cy treatment.
Liakopoulou
A
A
Health and Welfare Canada, Health Protection Branch, Ottawa, Ontario.
Buttar
H S
HS
Nera
E A
EA
Fernando
L
L
D000375:Aging / Q000276:immunology; D000818:Animals; D001835:Body Weight; D002860:Chromium Radioisotopes; D003520:Cyclophosphamide / Q000494:pharmacology*; D003877:Dermatitis, Contact / Q000188:drug therapy; D005260:Female; D007121:Immunocompetence / Q000187:drug effects*; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D051379:Mice; D012157:Mononuclear Phagocyte System / Q000502:physiology; D010047:Ovalbumin / Q000276:immunology; D010081:Oxazolone / Q000494:pharmacology; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D012156:Reticulocytes / Q000502:physiology; D012756:Sheep
D002860:Chromium Radioisotopes; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D010081:Oxazolone; D003520:Cyclophosphamide; D010047:Ovalbumin