title: Inhibition of oxygen-linked anion binding in Hb Camperdown [alpha 2 beta 2(104)(G6)Arg----Ser].
abstract: Oxygen equilibrium studies of purified Hb Camperdown [beta 104(G6) Arg----Ser] have revealed an increased oxygen affinity at acid pH, while it is decreased for pH values above 7.4. This accounts for an almost 40% reduction in the alkaline Bohr effect. The effects of chloride and organophosphate effectors on the oxygen affinity of Hb Camperdown are inhibited by 40-50%. In chloride-free Hepes buffer, Hb Camperdown exhibits a lower oxygen affinity than normal Hb A. The present results confirm the important role of the positively charged residues lining the beta 1 beta 2 interface in regulating the functional properties of hemoglobin.
authors.affiliation: INSERM U 299, Faculté de Médecine Paris-Sud, France.
authors.forename: J
authors.initials: J
authors.lastname: Kister
authors.forename: J
authors.initials: J
authors.lastname: Barbadjian
authors.forename: Y
authors.initials: Y
authors.lastname: Blouquit
authors.forename: B
authors.initials: B
authors.lastname: Bohn
authors.forename: F
authors.initials: F
authors.lastname: Galacteros
authors.forename: C
authors.initials: C
authors.lastname: Poyart
mesh_terms: D019794:2,3-Diphosphoglycerate; D000838:Anions / Q000378:metabolism*; D002712:Chlorides / Q000494:pharmacology; D004163:Diphosphoglyceric Acids / Q000494:pharmacology; D006453:Hemoglobinopathies / Q000097:blood / Q000235:genetics; D006455:Hemoglobins, Abnormal / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D010833:Phytic Acid / Q000494:pharmacology; D011086:Polycythemia / Q000097:blood / Q000235:genetics
chemical_list: D000838:Anions; D002712:Chlorides; D004163:Diphosphoglyceric Acids; D006455:Hemoglobins, Abnormal; D019794:2,3-Diphosphoglycerate; C044399:hemoglobin Camperdown; D010833:Phytic Acid; D010100:Oxygen