Identification of the antigenic components of paramyxovirus-3, paramyxovirus-6 and Newcastle disease virus in turkeys.
The aim of this study was to use the enzyme-linked immunosorbent assay (ELISA) and the Western immunoblotting as possible tools to differentiate infections in turkeys by different paramyxoviruses. Pooled hyperimmune sera of turkeys infected with either paramyxovirus-3 (PMV-3), paramyxovirus-6 (PMV-6), or Newcastle disease virus (NDV) were assayed for antibodies specific to the three viruses by the ELISA and Western immunoblotting. ELISA results showed cross reactions of turkey antibodies between PMV-3 and PMV-6 antigens, while turkey antibodies to NDV did not cross-react with any of the other paramyxoviruses. The immunoblots of sera from birds infected with PMV-3 (Minnesota turkeys and Iowa chickens) reacted to low molecular weight polypeptides of PMV-3 of 29, 32, and 34 kDa, and to a high molecular weight band of 200 kDa. The same Minnesota turkey sera had a cross reaction to the 200 kDa polypeptide of PMV-6, while the Iowa chicken sera did not. Both sera had no apparent reaction to NDV proteins. Western immunoblotting showed that the turkey PMV-3 sera had a specific reaction to a 220 kDa polypeptide present in PMV-3, but not in PMV-6, while the turkey PMV-6 sera had a specific reaction to a 130 kDa polypeptide present in PMV-6, but not in PMV-3. Immunoblots of pooled sera from turkeys infected with PMV-6 (Minnesota source) reacted to the 200 kDa protein present in both PMV-3 and PMV-6; however, no reaction occurred between this sera and NDV proteins.(ABSTRACT TRUNCATED AT 250 WORDS)
Barbour
E K
EK
College of Veterinary Medicine, University of Minnesota, St. Paul 55108.
Maniatas
J
J
Newman
J A
JA
Caputa
T
T
D000818:Animals; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000302:isolation & purification*; D015153:Blotting, Western; D003429:Cross Reactions; D003937:Diagnosis, Differential; D004797:Enzyme-Linked Immunosorbent Assay; D009521:Newcastle Disease / Q000175:diagnosis / Q000276:immunology*; D011201:Poultry Diseases / Q000175:diagnosis / Q000276:immunology*; D010253:Respirovirus Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D014422:Turkeys
D000914:Antibodies, Viral; D000956:Antigens, Viral