title: Culture conditions promoting dispersed growth and biphasic production of actinorhodin in shaken cultures of Streptomyces coelicolor A3(2).
abstract: Media and culture conditions were developed for experiments on the physiology of secondary metabolism in Streptomyces coelicolor A3(2). Well dispersed mycelial growth was obtained in a buffered starch-glutamate-salts medium; a high (5%) starch concentration and addition of glass beads aided dispersal. Under the conditions developed, production of actinorhodin was suppressed during trophophase growth and began abruptly near the growth maximum.
authors.affiliation: Biology Department, Dalhousie University, Halifax, Nova Scotia, Canada.
authors.forename: J L
authors.initials: JL
authors.lastname: Doull
authors.forename: L C
authors.initials: LC
authors.lastname: Vining
mesh_terms: D000880:Anthraquinones / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D003470:Culture Media; D013302:Streptomyces / Q000254:growth & development / Q000378:metabolism*; D000095622:Benzoisochromanequinones
chemical_list: D000880:Anthraquinones; D000900:Anti-Bacterial Agents; D003470:Culture Media; C013390:actinorhodin; D000095622:Benzoisochromanequinones