Rapid high-performance liquid chromatographic method for the measurement of the enantiomers of metoprolol in serum using a chiral stationary phase.
Metoprolol, a beta-adrenergic blocker, is only available as a racemic mixture for clinical use. A high-performance liquid chromatographic method for the separation of the optical isomers (enantiomers) in human serum is described utilizing a commercially available column with a cellulose tris (3,5-dimethylcarbamate) chiral stationary phase. Separation of the enantiomers is accomplished without precolumn derivatization using a mobile phase of hexane-2-propanol-diethylamine (91:8:1, v/v). The method can be successfully applied to pharmacokinetic studies in man.
Rutledge
D R
DR
Department of Pharmacy Practice, Wayne State University, College of Pharmacy, Detroit, MI 48202.
Garrick
C
C
D000328:Adult; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008297:Male; D008790:Metoprolol / Q000097:blood*; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D008790:Metoprolol