title: Species preference of bovine thymocytes and fibroblasts for bovine interleukin 1.
abstract: The purpose of this study was to determine whether bovine thymocytes and fibroblasts preferentially respond to bovine IL-1 as compared with human and murine interleukin 1 (IL-1). We compared equivalents units of recombinant murine and human IL-1 alpha, and purified monocyte-derived bovine IL-1, as measured in the murine thymocyte IL-1 assay, for their ability to stimulate the proliferation of bovine thymocytes and fibroblasts. Fetal and adult bovine thymocytes responded to bovine IL-1, but in contrast, did not respond to recombinant human or murine IL-1 alpha at the concentrations tested. Both adult and neonatal murine thymocytes responded similarly to the three IL-1 preparations. Thus, we concluded that the preferential response of bovine thymocytes for bovine IL-1 was not dependent upon the age of the thymus donor. Bovine fibroblasts also preferentially responded to bovine IL-1; fibroblast proliferation was stimulated by bovine but not human or murine IL-1. Our findings, therefore, imply that although IL-1 is highly conserved among mammalian species, its ability to stimulate the proliferation of thymocytes and fibroblasts may vary from species to species.
authors.affiliation: Department of Pathobiological Sciences, University of Wisconsin, Madison 53706.
authors.forename: J A
authors.initials: JA
authors.lastname: Lederer
authors.forename: C J
authors.initials: CJ
authors.lastname: Czuprynski
mesh_terms: D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000302:isolation & purification / Q000494:pharmacology*; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D013045:Species Specificity; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
chemical_list: D007375:Interleukin-1