Generation of species-specific antihemoglobin antibodies by immunization with synthetic peptides of human hemoglobin.
Four peptides (7-16 residues) representing nonconserved regions of human hemoglobin (Hb) were selected for synthesis by comparison of the amino acid sequence of human Hb with those of the most common domesticated animals. Mouse antisera resulting from immunization with the synthetic peptides were investigated for binding to a panel of animal Hbs using solid-phase radioimmunoassay (RIA). One of the peptides elicited antibodies which bound specifically to human Hb, but not to any Hb of the nonprimate animals tested. The results show that the peptide immunogen chosen on the basis of dissimilarity between regions of different species is useful for the generation of species-specific antibodies. Such antibodies could serve as valuable tools for clinical screening of fecal occult blood trait and for forensic identification of bloodstains of human origin.
Oshima
M
M
Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030.
Atassi
M Z
MZ
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000276:immunology*; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology; D002415:Cats; D002417:Cattle; D004285:Dogs; D004372:Ducks; D005260:Female; D006041:Goats; D006454:Hemoglobins / Q000276:immunology*; D006736:Horses; D006801:Humans; D007114:Immunization; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology*; D011323:Primates; D011817:Rabbits; D012756:Sheep; D013045:Species Specificity; D013552:Swine
D000906:Antibodies; D000941:Antigens; D006454:Hemoglobins; D010446:Peptide Fragments