[The use of immunoaffinity chromatography for isolation of carcinoembryonal antigen].
A procedure for cancer-embryonal antigen isolation involved extraction of the antigen by chloric acid, affinity chromatography on CNBr-Sepharose 4B containing immobilized antibodies and gel filtration on Sephadex G-200. Contaminating proteins, adsorbed on the immunosorbent were eluted with 0.05 M phosphate buffer, pH 7.4 containing 0.3% sodium cholate and 1 M NaCl, while cancer-embryonal antigen--with 0.1 M glycyl-HCl buffer, pH 2.2. After purification of the preparation on Sephadex G-200 homogenous fraction of the antigen, as shown by means of polyacrylamide gel electrophoresis in presence of SDS and by immunoblotting, was obtained. Yield of the antigen was about 40%.
Shcherban'
A I
AI
Lukashevich
L V
LV
Marchuk
S I
SI
Nezhinets
E A
EA
D002272:Carcinoembryonic Antigen / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods*; D006801:Humans; D009369:Neoplasms / Q000032:analysis*; D011863:Radioimmunoassay
D002272:Carcinoembryonic Antigen
Primenenie immunoaffinnoĭ khromatografii dlia vydeleniia rakovo-émbrional'nogo antigena.