Circular dichroic investigations into binding of sulfonamides to serum albumin.
The binding of twelve structurally related sulfonamides to serum albumins including human was investigated using a circular dichroic technique. Some differences of circular dichroic spectral characteristics were observed when sulfonamides were bound to the same albumin or when the drug was bound to several albumins. The differences in these circular dichroic characteristics may be due to various asymmetries. The Scatchard plots indicated that only the primary site was capable of inducing ellipticities of the drugs. The interaction with rabbit serum albumin showed significantly large binding constants and apparent anisotropy factors (g' values), in comparison with other albumins. No significant correlation between the g' values of the induced circular dichroic bands and partition coefficients or/and pKa values was observed. The induced ellipticities of the drug-albumin complexes decreased with pH. This pH dependence can be explained by the ionization of drug and albumin as well as the conformational change of the albumin.
Otagiri
M
M
Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
Nakamura
H
H
Imamura
Y
Y
Matsumoto
U
U
D000818:Animals; D002417:Cattle; D002712:Chlorides / Q000494:pharmacology; D002942:Circular Dichroism; D003956:Dialysis; D004285:Dogs; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011485:Protein Binding; D011817:Rabbits; D012709:Serum Albumin / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013449:Sulfonamides / Q000097:blood*
D002712:Chlorides; D012709:Serum Albumin; D013449:Sulfonamides; D012710:Serum Albumin, Bovine