title: Highly efficient immunoliposomes prepared with a method which is compatible with various lipid compositions.
abstract: Monoclonal antibody was conjugated to N-glutaryl-phosphatidylethanolamine in the presence of octylglucoside by using N-hydroxysulfosuccinimide as a carboxyl-activation reagent. The conjugated antibody was then incorporated into liposomes by a simple dialysis method. The method is mild and is compatible with various lipid compositions of the liposomes. We have prepared immunoliposomes containing a lung endothelium-specific monoclonal antibody and showed excellent target binding (approximately 75% injected dose) of the immunoliposomes in mouse. Immunoliposomes can be prepared to contain other acidic lipids such as phosphatidylserine and various amounts of cholesterol. The presence of 20% or more cholesterol in liposomes resulted in high level of target binding. We have used in these experiments a new radioactive lipid-phase marker, 111In-DTPA-SA, which was very stable in vivo. The halflife of clearance in mouse exceeded 3 weeks.
authors.affiliation: Department of Biochemistry, University of Tennessee, Knoxville 37996.
authors.forename: E
authors.initials: E
authors.lastname: Holmberg
authors.forename: K
authors.initials: K
authors.lastname: Maruyama
authors.forename: D C
authors.initials: DC
authors.lastname: Litzinger
authors.forename: S
authors.initials: S
authors.lastname: Wright
authors.forename: M
authors.initials: M
authors.lastname: Davis
authors.forename: G W
authors.initials: GW
authors.lastname: Kabalka
authors.forename: S J
authors.initials: SJ
authors.lastname: Kennel
authors.forename: L
authors.initials: L
authors.lastname: Huang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage; D002784:Cholesterol / Q000032:analysis; D004727:Endothelium / Q000276:immunology; D007205:Indium Radioisotopes; D007700:Kinetics; D008081:Liposomes; D008168:Lung / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D004369:Pentetic Acid; D010713:Phosphatidylcholines / Q000032:analysis; D010714:Phosphatidylethanolamines; D010718:Phosphatidylserines / Q000032:analysis; D051381:Rats; D014018:Tissue Distribution
chemical_list: D000911:Antibodies, Monoclonal; D007205:Indium Radioisotopes; D008081:Liposomes; C049392:N-glutarylphosphatidylethanolamine; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010718:Phosphatidylserines; D004369:Pentetic Acid; D002784:Cholesterol