title: Role of adjuvants in inhibitory influence of immunization with porcine zona pellucida antigen (ZP-3) on ovarian folliculogenesis in bonnet monkeys: a morphological study.
abstract: Female bonnet monkeys were immunized with 55 kDa porcine zona antigen (ZP-3), with either complete Freund's adjuvant (CFA) or sodium phthalylated lipopolysaccharide (SPLPS) as adjuvant. Anti-ZP-3 antibody titers were monitored and the effect of immunization on the ovarian morphology was assessed by high-resolution light microscopy. The study demonstrated that both adjuvants used were equally potent in eliciting antibody response against ZP-3. Although no morphological damage to ovarian components was observed in animals immunized with SPLPS as adjuvant, immunization using CFA resulted in profound ovarian follicular atrophy, sparing only the primordial follicles. The atrophic phenomenon involved those follicles that either already had or were in the process of forming zona pellucida. The results of this study indicate that choice of adjuvant may be an important consideration for immunization against zona antigens. These findings encourage further investigations for developing better immunization regimen aimed at using zona antigens for immunocontraception.
authors.affiliation: National Institute of Immunology, New Delhi, India.
authors.forename: S N
authors.initials: SN
authors.lastname: Upadhyay
authors.forename: P
authors.initials: P
authors.lastname: Thillaikoothan
authors.forename: A
authors.initials: A
authors.lastname: Bamezai
authors.forename: S
authors.initials: S
authors.lastname: Jayaraman
authors.forename: G P
authors.initials: GP
authors.lastname: Talwar
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000906:Antibodies / Q000032:analysis; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology*; D005260:Female; D005620:Freund's Adjuvant / Q000494:pharmacology; D007114:Immunization; D008070:Lipopolysaccharides / Q000494:pharmacology; D008255:Macaca radiata; D006080:Ovarian Follicle / Q000276:immunology / Q000473:pathology*; D010063:Ovum / Q000276:immunology*; D010795:Phthalic Acids / Q000494:pharmacology; D013552:Swine; D015044:Zona Pellucida / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000906:Antibodies; D000941:Antigens; D008070:Lipopolysaccharides; D010795:Phthalic Acids; C032279:phthalic acid; D005620:Freund's Adjuvant