[Chromatographic identification and analysis of dextromoramide in the plasma by the method of high performance liquid chromatography].
Dextromoramide and pethidine were separated and identified by thin-layer chromatography on silica gel, using ammonia and methanol (1.5:100) as the mobile phase, after previous extraction with dicthyl ether or with a mixture of n-hexane and isoamyl alcohol (98.5:1.5) from blood alkalized to pH 10.3 Dextromoramide can be revealed on the chromatograms in the amount of 0.5 micrograms and pethidine in the amount of 1 micrograms using the Dragendorff reagent. Reversed-phase TLC proved less sensitive. High-performance liquid chromatography on the column of LiChrosorb RP-18 was applied to the determination of dextromoramide in blood after extraction with diethyl ether, using methanol--phosphate buffer pH 4.5 (95:5) as the mobile phase. The determination range was of 0.5-5.0 micrograms per 2 cm3 of blood plasma (1.26.10(-8)-1.26.10(-7) mole/dm3).
Misztal
G
G
Przyborowski
L
L
Jednacz
T
T
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D003916:Dextromoramide / Q000097:blood* / Q000302:isolation & purification; D006801:Humans
D003916:Dextromoramide
Identyfikacja chromatograficzna i oznaczanie dekstromoramidu w osoczu metoda wysokosprawnej chromatograffii cieczowej.