The potential role of platelet-derived growth factor as an autocrine or paracrine factor for human bone cells.
Platelet-derived growth factor, PDGF, is a potent mitogen for cells of mesenchymal origin such as fibroblasts, smooth muscle cells and glial cells. PDGF is thought to have the potential to act as both a paracrine and an autocrine factor. Studies described here extend these observations to human bone-derived cells. Exogenous PDGF induces biologic activity in two human osteogenic sarcoma cell lines and in one of these, the two PDGF genes, PDGF-1 and PDGF-2/c-sis are expressed. In addition, PDGF stimulates proliferation of normal osteoblastic cells derived from adult human cancellous bone. The expression of the PDGF-1 gene but not the PDGF-2/c-sis gene is demonstrated in normal human adult bone-derived cells by Northern blot analysis and synthesis of PDGF is shown by immunoprecipitation with PDGF antisera. These studies indicate that PDGF has the potential to act as a paracrine or autocrine regulator of bone cells.
Graves
D T
DT
Department of Oral Biology and Periodontology, Boston University School of Graduate Dentistry, MA 02118.
Valentin-Opran
A
A
Delgado
R
R
Valente
A J
AJ
Mundy
G
G
Piche
J
J
D015152:Blotting, Northern; D001842:Bone and Bones / Q000187:drug effects / Q000502:physiology*; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D015870:Gene Expression; D006801:Humans; D010006:Osteoblasts / Q000378:metabolism; D012516:Osteosarcoma / Q000378:metabolism / Q000473:pathology; D010982:Platelet-Derived Growth Factor / Q000235:genetics / Q000494:pharmacology / Q000502:physiology*; D011233:Precipitin Tests; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000473:pathology
D010982:Platelet-Derived Growth Factor