[A model for studying the transmission of information produced by certain growth factors: activation mechanisms of S6 kinase in cultured astrocytes].
Treatment of cultured astrocytes from 2-day-old rat cerebral hemispheres with insulin, somatomedin C (IGF1), thrombin and acidic or basic fibroblast growth factors promoted a rapid activation of a cytosolic protein kinase (S6 kinase) which phosphorylates ribosomal protein S6. The phorbol ester (TPA) also triggered a rapid increase in S6 kinase activity. Two agonists of adenylate cyclase activity (forskolin and isoproterenol) and the cyclic AMP analog (dibutyryl cAMP) also stimulated the same S6 kinase. These observations support the idea that several pathways might promote the activation of the same entity that is regarded as one of the primary targets of signals elicited by growth factors.
Toru-Delbauffe
D
D
INSERM U. 96, unité de recherche sur la glande thyroïde et la régulation hormonale, Le Kremlin-Bicêtre, France.
Gavaret
J M
JM
Matricon
C
C
Pomerance
M
M
Jacquemin
C
C
Pierre
M
M
D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000166:cytology* / Q000201:enzymology; D002455:Cell Division; D002478:Cells, Cultured; D004789:Enzyme Activation / Q000502:physiology; D008954:Models, Biological; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D019893:Ribosomal Protein S6 Kinases
D011494:Protein Kinases; D019893:Ribosomal Protein S6 Kinases
Un modèle pour l'étude de la transmission de l'information produite par certains facteurs de croissance: les mécanismes d'activation de la S6 kinase dans les astrocytes en culture.