title: Human chromosome banding with restriction endonucleases HaeIII, HinfI and PstI.
abstract: Human chromosome banding was carried out with restriction endonucleases (REs) HaeIII, HinfI, PstI and the effects of some factors on it were examined. HaeIII induced C and G banding patterns, HinfI induced negative C bands but the centromeric heterochromatin of chromosomes 3 and 4 remained selectively dark-stained. A new heteromorphism of C banded region was discovered in chromosome 4. PstI induced G-like banding pattern. The duration of enzymatic digestion, the concentration of glycerol in the reaction buffer, as well as the ageing and heating of chromosome preparations all had a significant influence on the banding effects of the REs.
authors.affiliation: Department of Medical Genetics, West China University of Medical Sciences, Chengdu.
authors.forename: S Z
authors.initials: SZ
authors.lastname: Zhang
authors.forename: W F
authors.initials: WF
authors.lastname: Dong
mesh_terms: D002871:Chromosome Banding / Q000379:methods; D015252:Deoxyribonucleases, Type II Site-Specific; D006801:Humans; D007231:Infant, Newborn; D008677:Metaphase
chemical_list: C024388:CTGCAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; C032229:GANTC-specific type II deoxyribonucleases; C024403:GGCC-specific type II deoxyribonucleases