title: Suppression of avidin processing and presentation by mouse macrophages after sublethal exposure to dieldrin.
abstract: The molecular events in macrophage antigen processing and presentation were examined to determine the possible site(s) of cell-xenobiotic interaction. Antigenic processing by mouse peritoneal macrophages of a single protein antigen, avidin, was significantly suppressed following sublethal exposure of animals to an organochlorine pesticide, dieldrin. Exposure of C57B1/6 female mice to dieldrin affected the in vitro uptake of [methyl-14C]avidin by peritoneal macrophages and markedly decreased phagocytosis of fluorescein-labelled microspheres and Salmonella typhimurium. Release of the processed avidin, determined by immunochemical quantification of immunogenic avidin and by bioassay of immunogenicity of the released antigen, was also markedly affected. Dieldrin markedly affected presentation of avidin on the macrophage surface, observed by cytoimmunochemical staining of the antigen with fluorescent antibody and flow cytometry. Inhibition of the release of processed avidin was dieldrin dose- and time-dependent, following single sublethal intraperitoneal (ip) exposure to the pesticide. The antigenic properties of processed avidin, determined by biological assay using lymphocyte cultures of normal C57B1/6 mice primed with avidin, were proportional to the antigen concentration in supernatants of macrophage cultures, for both vehicle controls and dieldrin-exposed animals. This observation and analysis of the kinetics of release of processed avidin by macrophages from control and dieldrin-exposed animals suggested that the release of processed avidin, but not the immunogenicity of the antigen itself, was affected by the pesticide exposure. Generally, impairment of avidin processing and presentation appeared to be more dramatic than other pesticide-related injuries to macrophages, such as the uptake of the antigen. In conclusion, antigen processing could be a sensitive target for dieldrin-related injury of macrophage functional activities, which, in consequence, could produce suppression of the humoral immune response.
authors.affiliation: Département des Sciences Biologiques, Université du Québec à Montréal, Québec, Canada.
authors.forename: K
authors.initials: K
authors.lastname: Krzystyniak
authors.forename: D
authors.initials: D
authors.lastname: Flipo
authors.forename: S
authors.initials: S
authors.lastname: Mansour
authors.forename: M
authors.initials: M
authors.lastname: Fournier
mesh_terms: D000818:Animals; D001360:Avidin / Q000276:immunology / Q000378:metabolism*; D004026:Dieldrin / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005260:Female; D007108:Immune Tolerance; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D010587:Phagocytosis / Q000187:drug effects; D013601:T-Lymphocytes / Q000502:physiology; D013997:Time Factors
chemical_list: D001360:Avidin; D004026:Dieldrin