Synthesis of DNA fragments bearing 7,8-dihydro-8-oxo-adenine: a gamma radiation product.
In order to investigate the mutagenic effects and the repair of DNA lesions induced by gamma rays, 7,8-dihydro-8-oxo-adenine was introduced in DNA fragments using cyanoethyl phosphoroamidite method. The modified oligonucleotides were purified by HPLC and the presence of the defect in the final product was confirmed by DNA sequencing, enzymatic hydrolysis and FAB mass spectrometry.
Guy
A
A
Département de Recherche Fondamentale, Centre d'Etudes Nucléaires de Grenoble, France.
Molko
D
D
Téoule
R
R
D000225:Adenine / Q000031:analogs & derivatives*; D004247:DNA / Q000528:radiation effects; D005720:Gamma Rays; D008956:Models, Chemical; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*
D009838:Oligodeoxyribonucleotides; C002992:8-hydroxyadenine; D004247:DNA; D000225:Adenine