DNA binding proteins of Schistosoma mansoni recognizing a hexanucleotide motif occurring in genes regulated by steroids.
1. Schistosome proteins interacting with a gene (F-10), which is only expressed in adult females, were investigated. 2. These were also tested using a synthetic oligonucleotide of 20 bp bearing a defined sequence derived from the F-10 gene and containing a hexanucleotide motif, TGTCCT, occurring in genes responsive to steroids. 3. Schistosome proteins (male and female) bound to the F-10 DNA, but only the male proteins bound to the synthetic oligonucleotide with high affinity. 4. The other preparations each produced different binding patterns, although this seemed to lack specificity. 5. These results indicated that the F-10 gene binds different proteins along its structure and suggested that proteins present in the male schistosomes may regulate its expression.
Rumjanek
F D
FD
Departamento de Bioquimica-ICB/CCS, Universidade Federal do Rio de Janeiro, Brazil.
Braga
V M
VM
Kelly
C
C
D000818:Animals; D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005260:Female; D005796:Genes / Q000187:drug effects; D009838:Oligodeoxyribonucleotides / Q000378:metabolism; D011485:Protein Binding; D012550:Schistosoma mansoni / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D013256:Steroids / Q000494:pharmacology
D004268:DNA-Binding Proteins; D009838:Oligodeoxyribonucleotides; D013256:Steroids