Cloning and analysis of a deletable tetracycline-resistance determinant of Streptomyces lividans 1326.
An unstable determinant encoding resistance to tetracycline and minocycline has been cloned and characterized. It could be demonstrated that this determinant shares extensive homology with the otrA gene identified in Streptomyces rimosus. Tetracycline-sensitive variants of Streptomyces lividans derive mostly by deletion of this resistance determinant and neighbouring DNA regions.
Kessler
A
A
Institut für Genetik und Mikrobiologie, Universität München, FRG.
Dittrich
W
W
Betzler
M
M
Schrempf
H
H
D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D009693:Nucleic Acid Hybridization; D010444:Peptide Elongation Factor Tu / Q000235:genetics; D010641:Phenotype; D010957:Plasmids; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013291:Streptococcus / Q000235:genetics; D013302:Streptomyces / Q000187:drug effects / Q000235:genetics*; D013753:Tetracycline Resistance / Q000235:genetics*; D014169:Transformation, Bacterial
D010444:Peptide Elongation Factor Tu