title: Effect of vaccination with a pentavalent leptospiral vaccine containing Leptospira interrogans serovar hardjo type hardjo-bovis on type hardjo-bovis infection of cattle.
abstract: Effectiveness of 2 pentavalent leptospiral vaccines containing Leptospira interrogans serovar hardjo was evaluated for protection of steers from infection with serovar hardjo type hardjo-bovis. The hardjo component of 1 vaccine was prepared from serovar hardjo type hardjoprajitno. The hardjo component of the other vaccine was prepared from serovar hardjo type hardjo-bovis. Two steers were vaccinated once and 4 steers were vaccinated twice with the pentavalent vaccine containing type hardjoprajitno. Four steers were vaccinated once and 4 steers were vaccinated twice with the pentavalent vaccine containing type hardjo-bovis. Four steers were maintained as non-vaccinated controls. Steers given vaccine containing type hardjo-bovis developed higher mean serum microscopic agglutination titers against serovar hardjo than steers given vaccine containing hardjoprajitno. Six months after the first vaccination, all steers were challenge-exposed on 3 occasions by conjunctival instillation of 10(7) serovar hardjo type hardjo-bovis organisms, and on 1 occasion by conjunctival instillation of urine from a steer shedding hardjo-bovis. All control and all vaccinated steers became infected and shed serovar hardjo type hardjo-bovis in the urine. Lesions were detected in kidneys of 3 of 4 nonvaccinated control steers, 5 of 6 steers given hardjoprajitno vaccine, and 6 of 8 steers given hardjo-bovis vaccine. Leptospires were detected in kidneys of 4 of 4 control steers and 13 of 14 vaccinated steers.
authors.affiliation: Agricultural Research Service, National Animal Disease Center, Ames, IA 50010.
authors.forename: C A
authors.initials: CA
authors.lastname: Bolin
authors.forename: R L
authors.initials: RL
authors.lastname: Zuerner
authors.forename: G
authors.initials: G
authors.lastname: Trueba
mesh_terms: D000818:Animals; D000917:Antibody Formation; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology*; D007921:Leptospira interrogans / Q000145:classification / Q000276:immunology*; D007922:Leptospirosis / Q000276:immunology / Q000662:veterinary*; D008297:Male; D012703:Serotyping / Q000662:veterinary; D013045:Species Specificity; D014611:Vaccination / Q000662:veterinary
chemical_list: D001428:Bacterial Vaccines