An incubation system for the NMR study of kidney tubules.
Isolated kidney cortical tubules require a very rapid oxygen supply and mechanical agitation to be optimally functional. A sample chamber in which a tubule suspension is oxygenated by recirculating oxygen gas inside a coil of dialysis fibers to avoid cell loss through bubbling and in which the tubules are agitated by a gas-driven turbine has been designed. In such a system, dog cortical tubules (35-45 mg/ml) were found to be metabolically stable for more than 3 h as indicated by linear lactate consumption and glucose production. Small pH variations resulting from carbon dioxide and bicarbonate productions were measured. Good-quality 23Na NMR spectra of dog kidney cortical tubules were recorded with such a system, allowing a 1-min time resolution.
Ammann
H
H
Département de Physiologie, Université de Montréal, Quebec, Canada.
Boulanger
Y
Y
Legault
P
P
Vinay
P
P
D000818:Animals; D004285:Dogs; D004867:Equipment Design; D005665:Furosemide / Q000494:pharmacology; D066298:In Vitro Techniques; D007684:Kidney Tubules / Q000166:cytology / Q000378:metabolism*; D007773:Lactates / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D010101:Oxygen Consumption / Q000187:drug effects; D010477:Perfusion / Q000295:instrumentation / Q000379:methods; D012964:Sodium
D007773:Lactates; D005665:Furosemide; D012964:Sodium