Agarose gel electrophoresis in isozyme separation and visualization.
Isozyme analysis of rodent-human somatic cell hybrids has been used frequently to detect specific human chromosomes. The majority of these isozyme systems employs starch gels, the use of which can be laborious when screening large numbers of cell lines. We describe the development of two procedures to detect the long arms of human chromosomes 1 and 2 in Chinese hamster-human cell hybrids by a rapid and reproducible method using 1-mm-thick agarose gels. Detection of human chromosome 1q was accomplished by screening for human fumarate hydratase activity, whose gene has been mapped to 1q42.1. Detection of chromosome 2q was performed by screening for the isozyme isocitrate dehydrogenase 1, which has been localized to 2q32-qter. These systems provide a basis for the further development of procedures for detecting chromosome-specific isozyme markers in agarose gels.
Hunt
J D
JD
Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, Memphis, Tennessee 38101.
Goren
M P
MP
Tereba
A
A
D000818:Animals; D002878:Chromosomes, Human, Pair 1; D002889:Chromosomes, Human, Pair 2; D006224:Cricetinae; D003412:Cricetulus; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005649:Fumarate Hydratase / Q000032:analysis; D006801:Humans; D006822:Hybrid Cells; D007521:Isocitrate Dehydrogenase / Q000235:genetics; D007527:Isoenzymes / Q000235:genetics / Q000302:isolation & purification*; D009447:Neuroblastoma; D014407:Tumor Cells, Cultured
D007527:Isoenzymes; D007521:Isocitrate Dehydrogenase; D005649:Fumarate Hydratase