[Primary structure of the leading sequence of 16S rRNA gene in Brassica napus chloroplast].
The 840bp leading sequence of 16S rRNA gene (including 140bp 16S rRNA gene) was sequenced. Screening for structure elements common to tRNA reveals a gene coding for tRNA(Val). An open reading frame, and three E. coli RNA polymerase binding sites were found. In front of the open reading frame, the tRNA(Val) gene, and the 16S rRNA gene, a stable stem-loop structure can be formed. We suggested that these stem-loop structures may have some effect on the gene transcription which are located on the inverted repeat sequence in chloroplast.
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D001483:Base Sequence; D001937:Brassica / Q000166:cytology / Q000235:genetics*; D002736:Chloroplasts; D017343:Genes, Plant; D008969:Molecular Sequence Data; D015394:Molecular Structure; D012335:RNA, Ribosomal / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*
D012335:RNA, Ribosomal; D012336:RNA, Ribosomal, 16S