Influence of steroid hormones on protein-platelet interaction at the blood-polymer interface.
To develop artificial materials for prolonged use in the vascular system, the complicated process of surface-induced thrombosis needs to be better understood. Steroidal hormones have a profound role in thrombosis and haemostasis, although adequate studies are not available to demonstrate their part in the thromboembolic phenomena that occur at the blood-foreign material interface. We studied the interfacial phenomena of five steroid hormonal drugs, Sustanon, Menstrogen, Mixogen, Durabolin and Ovral and their interaction with proteins and platelets toward an artificial surface using contact angle, polyacrylamide gel electrophoresis, trace labelling methods, etc. This study demonstrates the effect of these hormones to modulate platelet-surface attachment in the presence of platelet inducers. The addition of steroid hormones to the polymer-protein system can inhibit the level of surface-bound albumin where the fibrinogen binding to an artificial surface has been enhanced or unaltered. Steroids also increase platelet-surface attachment to variable degrees. Prolonged use of steroids or the oestrogen-containing oral contraceptive agents may not be advisable for patients having an artificial implant in contact with blood.
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Biosurface Technology Division, BMT Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Poojapura, Trivandrum, India.
Chandy
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D000327:Adsorption; D000818:Animals; D001798:Blood Proteins / Q000493:pharmacokinetics*; D002417:Cattle; D003277:Contraceptives, Oral, Combined / Q000494:pharmacology; D003738:Dental Cements; D004338:Drug Combinations / Q000494:pharmacology; D004958:Estradiol / Q000031:analogs & derivatives / Q000494:pharmacology; D004997:Ethinyl Estradiol / Q000494:pharmacology; D019304:Ethinyl Estradiol-Norgestrel Combination; D005003:Ethisterone / Q000494:pharmacology; D006728:Hormones / Q000494:pharmacology*; D066298:In Vitro Techniques; D009277:Nandrolone / Q000031:analogs & derivatives / Q000494:pharmacology; D009644:Norgestrel / Q000494:pharmacology; D010973:Platelet Adhesiveness / Q000187:drug effects*; D011076:Polycarboxylate Cement; D011374:Progesterone / Q000494:pharmacology; D013256:Steroids / Q000494:pharmacology*; D013739:Testosterone / Q000031:analogs & derivatives / Q000494:pharmacology
D001798:Blood Proteins; D003277:Contraceptives, Oral, Combined; D003738:Dental Cements; D004338:Drug Combinations; D006728:Hormones; C055250:Menstrogen; D011076:Polycarboxylate Cement; D013256:Steroids; C054240:estradiol benzoate, estradiol phenylpropionate, testosterone phenylpropionate, testosterone propionate drug combination; C005506:polycarbonate; D009644:Norgestrel; D013739:Testosterone; D004997:Ethinyl Estradiol; D011374:Progesterone; D004958:Estradiol; D009277:Nandrolone; D019304:Ethinyl Estradiol-Norgestrel Combination; C008580:nandrolone phenpropionate; D005003:Ethisterone