title: [Amino acid sequence of various peptides of tryptophanyl-tRNA-synthetase from bovine pancreas].
abstract: Method of isolation of the bovine pancreas tryptophanyl-tRNA synthetase is improved and a protein with greater than or equal to 99% purity, according to PAGE-SDS, is obtained. The pure enzyme is digested with clostripain and the hydrolysate is separated by FPLC anion-exchange chromatography followed by reversed phase HPLC. Amino acid sequences of 6 individual peptides, including C-terminal one, were determined by the automated Edman degradation. A peptide is also revealed which is encoded with the low degeneracy.
authors.forename: T A
authors.initials: TA
authors.lastname: Zargarova
authors.forename: G K
authors.initials: GK
authors.lastname: Kovaleva
authors.forename: O O
authors.initials: OO
authors.lastname: Favorova
authors.forename: N B
authors.initials: NB
authors.lastname: Levina
authors.forename: I N
authors.initials: IN
authors.lastname: Telezhinskaia
mesh_terms: D000595:Amino Acid Sequence; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D008969:Molecular Sequence Data; D010179:Pancreas / Q000201:enzymology*; D010455:Peptides / Q000235:genetics* / Q000302:isolation & purification; D011487:Protein Conformation; D014369:Tryptophan-tRNA Ligase / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D010455:Peptides; D000604:Amino Acyl-tRNA Synthetases; D014369:Tryptophan-tRNA Ligase
vernacular_title: Aminokislotnaia posledovatel'nost' nekotorykh peptidov triptofanil- tRNK-sintetazy iz podzheludochnoĭ zhelezy krupnogo rogatogo skota.