[Changes induced by sarcolysine in vivo in the composition of DNA-bound lipids in sarcoma 37].
The two DNA fractions were isolated from sarcoma 37 by the use of the phenol method: supramolecular complex of DNA (SC DNA, 60%) and "phenol" nuclear matrix DNA (PNM DNA, 40%). The lipids in SC DNA represented of light and tightly bound components, the latter was similar to the lipid composition of PNM DNA. SC DNA contains 20 micrograms of neutral lipids (NL) and 6.5 micrograms of phospholipids (PL), while PNM DNA contains 9.8 micrograms of NL and 3.5 micrograms of PL per mg DNA. SC DNA-bound lipids of sarcoma 37 are deficient in free cholesterol (FC, 13%), but rich in cholesterol esters (CE, 39%) and free fatty acids (FFA, 23%); very rich in cardiolipin (CL, 43%) and phosphatidylethanolamine (PE, 28%), but deficient in phosphatidylcholine (PC, 12%). The tumor contains triglycerides (TG) that is absent in DNA of the normal cells. The injection of sarcolysine (10 micrograms/kg) markedly increased (1.5-3 times) the content of all LN and PL fractions in SC DNA, which was accompanied by both the accumulation of FC, TG, PC and the reduction of the remaining lipid fractions in PNM DNA. It is supposed, that DNA-bound lipids may be the target for the action of sarcolysine.
Struchkov
V A
VA
Strazhevskaia
N V
NV
D000818:Animals; D002855:Chromatography, Thin Layer; D004273:DNA, Neoplasm / Q000032:analysis / Q000187:drug effects* / Q000302:isolation & purification; D004353:Drug Evaluation, Preclinical; D008055:Lipids / Q000032:analysis* / Q000302:isolation & purification; D008297:Male; D008558:Melphalan / Q000627:therapeutic use*; D051379:Mice; D012511:Sarcoma 37 / Q000032:analysis* / Q000188:drug therapy; D012513:Sarcoma, Experimental / Q000032:analysis*
D004273:DNA, Neoplasm; D008055:Lipids; D008558:Melphalan
Izmenenie sostava DNK-sviazannykh lipidov sarkomy 37, indutsirovannoe sarkolizinom in vivo.