Estimation of physiological volumes in the isolated perfused porcine skin flap.
A requirement for pharmacokinetic modeling of isolated perfused organ systems used for in vitro to in vivo extrapolations is a measure of the organ's physiological spaces. Two methods were employed to estimate intravascular and extracellular spaces in four isolated perfused porcine skin flaps (IPPSF) from 2 pigs. One of these methods used regression techniques based on a one-compartment model, and was found to be a good estimator of intravascular volume only. The other method was a non-parametric numerical integration technique, and was found to give reasonable estimates for both intravascular and extracellular volumes. The latter method's estimate for extracellular space was 0.20 +/- 0.03 mL/g, and the estimated vascular space by both methods was 0.08 +/- 0.01 mL/g.
Williams
P L
PL
Cutaneous Pharmacology and Toxicology Center, College of Veterinary Medicine, North Carolina State University, Raleigh 27606.
Riviere
J E
JE
D000818:Animals; D066298:In Vitro Techniques; D007444:Inulin; D010477:Perfusion; D012710:Serum Albumin, Bovine / Q000378:metabolism; D012867:Skin / Q000033:anatomy & histology; D012879:Skin Physiological Phenomena; D013552:Swine
D012710:Serum Albumin, Bovine; D007444:Inulin