title: Gene amplification associated with the dominant cob-354 cobalt resistance trait in Dictyostelium discoideum.
abstract: A DNA amplification is correlated with the dominant, unstable cob-354 cobalt resistance trait in the cellular slime mold, Dictyostelium discoideum. The amplified DNA is present as about 50 copies of an extrachromosomal element. Cells grown under nonselective conditions in the absence of cobalt ions lose both the cobalt resistance trait and all extrachromosomal copies of the amplified DNA. The amplified DNA is transferrable to new genetic backgrounds by parasexual genetic crosses. These results explain the inability to map the cob-354 trait to a linkage group. The chromosomal origin of the amplified DNA is group III or VI. Thus the resistance trait appears to be independent of the previously known cobalt resistance locus, cobA, which maps to group VII. A developmental defect involving the production of multiply-tipped aggregates that do not complete fruiting body formation also is correlated with the presence of the amplified DNA.
authors.affiliation: Department of Biology, Utah State University, Logan 84322-5500.
authors.forename: S L
authors.initials: SL
authors.lastname: Jensen
authors.forename: H
authors.initials: H
authors.lastname: Ashktorab
authors.forename: J E
authors.initials: JE
authors.lastname: Hughes
authors.forename: D L
authors.initials: DL
authors.lastname: Welker
mesh_terms: D000818:Animals; D003035:Cobalt / Q000494:pharmacology*; D004271:DNA, Fungal / Q000302:isolation & purification; D004023:Dictyostelium / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D004352:Drug Resistance, Microbial / Q000235:genetics*; D004586:Electrophoresis; D005111:Extrachromosomal Inheritance; D005784:Gene Amplification; D005799:Genes, Dominant; D008040:Genetic Linkage; D005819:Genetic Markers
chemical_list: D004271:DNA, Fungal; D005819:Genetic Markers; D003035:Cobalt