The inhibitory effect of hydrocortisone on interferon production by rat spleen cells.
The effect of hydrocortisone on interferon r(IFN-r) production by rat spleen cells and its mechanism were studied. The results showed that hydrocortisone inhibited IFN-r production at concentrations as low as 5.52 x 10(-10) M, with complete suppression at 5.52 x 10(-8) M, and the total number and survival rate of the cultured spleen cells were not apparently affected by 5.52 x 10(-8) M hydrocortisone. The inhibitory effect was dose-dependent when the concentration was from 5.52 x 10(-10) M to 5.52 x 10(-8) M and could be blocked by RU38486, a competitive antagonist of glucocorticoid. Our results suggested that glucocorticoid may inhibit IFN-r production through a receptor-mediated mechanism.
Ding
J Y
JY
Department of Pathophysiology, Second Military Medical College, Shanghai, China.
Yang
S K
SK
Xu
R B
RB
D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D006854:Hydrocortisone / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D007372:Interferons / Q000096:biosynthesis*; D015735:Mifepristone / Q000494:pharmacology; D011043:Pokeweed Mitogens / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D011043:Pokeweed Mitogens; D015735:Mifepristone; D007372:Interferons; D006854:Hydrocortisone