Fractionation of cock spermatozoa.
An account is given of the methodology for fractionation of cock spermatozoa into head and tail fractions by ultrasonication, followed by sucrose density gradient centrifugation. Quantitative estimates of DNA attested to 89.4% purity of the head fraction and low contamination of tails with heads. Recovery of protein and malic dehydrogenase (MDH) activity, following sperm fractionation, averaged 94.3% and 95.7%, respectively. Contamination of the head fraction with tails, as assessed by MDH assay, was only 4.65%, and the purity of the tail fraction was 91%. Intensive succinic dehydrogenase (SDH) activity was histochemically localised in the separated tail fraction and in the tail portion of intact spermatozoa. However, SDH activity was discernible neither in the head fraction nor in the head of intact spermatozoa.
Gehlaut
B S
BS
Mangle
N S
NS
Datta
I C
IC
D000818:Animals; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D002645:Chickens / Q000502:physiology*; D008297:Male; D013010:Sonication; D013094:Spermatozoa / Q000032:analysis*