Establishment of anti-idiotypic monoclonal antibodies against anti-interphotoreceptor retinoid-binding protein monoclonal antibody.
Two monoclonal antibodies (MAbs) against interphotoreceptor retinoid-binding protein (IRBP) were previously established. One of the antibodies designated as MAb-TRA4 reacted with the IRBP of multiple species. Using this MAb-TRA4, 6 clonal anti-idiotypic monoclonal antibodies (MAb-TRDy; y = 1-6) against MAb-TRA4 were established. All of the MAb-TRDy were characterized by ELISA, immunoblotting and immunohistochemical studies. It was shown by ELISA that MAb-TRDy inhibited the specific binding between IRBP and MAb-TRA4 and did not cause nonspecific binding to rat polyclonal immunoglobulins (IgG and IgM). Immunoblotting assay demonstrated that MAb-TRDy inhibited the binding between IRBP and MAb-TRA4. Immunohistochemical examination also confirmed the immunological characterization of MAb-TRDy. Thus the present results indicate that the MAb-TRDy which have been established are the anti-idiotypic MAbs against anti-IRBP MAb-TRA4. MAb-TRDy can be used as a tool to study the pathogenesis of experimental autoimmune uveitis induced by IRBP.
Abe
T
T
Department of Ophthalmology, Akita University School of Medicine, Japan.
Yamaki
K
K
Sakuragi
S
S
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D001327:Autoimmune Diseases / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005136:Eye Proteins; D005260:Female; D006825:Hybridomas; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000276:immunology; D007132:Immunoglobulin Isotypes / Q000276:immunology; D051381:Rats; D011917:Rats, Inbred Lew; D012160:Retina / Q000276:immunology; D012177:Retinol-Binding Proteins / Q000276:immunology*; D014605:Uveitis / Q000276:immunology*; D000099214:Retinol-Binding Proteins, Interstitial
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D005136:Eye Proteins; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D012177:Retinol-Binding Proteins; D000099214:Retinol-Binding Proteins, Interstitial