A method for detection of cellulases in polyacrylamide gels using 5-bromoindoxyl-beta-D-cellobioside: high sensitivity and resolution.
The assay of endo-1,4-beta-glucanases (cellulases) from Trichoderma reesei, T. longibrachiatum, and Sporotrichum pulverulentum by 5-bromoindoxyl-beta-D-cellobioside is described. The substrate is enzymatically cleaved to afford 5-bromoindoxyl and latter undergoes immediate azo coupling with Fast Red or oxidation by nitroblue monotetrazolium chloride, various forms of endoglucanases which can thus be assayed in polyacrylamide gel.
Chernoglazov
V M
VM
Department of Chemistry, Moscow State University, USSR.
Ermolova
O V
OV
Vozny
Y V
YV
Klyosov
A A
AA
D000180:Acrylic Resins / Q000032:analysis*; D002475:Cellobiose / Q000031:analogs & derivatives; D002480:Cellulase / Q000032:analysis* / Q000378:metabolism; D004187:Disaccharides; D004591:Electrophoresis, Polyacrylamide Gel; D005069:Evaluation Studies as Topic; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000032:analysis
D000180:Acrylic Resins; D004187:Disaccharides; D007527:Isoenzymes; C016680:polyacrylamide gels; C062427:5-bromoindoxyl-beta-cellobioside; D002475:Cellobiose; D002480:Cellulase