title: A comparison of erythrocytes, lymphocytes and blood plasma as samples in fatty acid analysis for the diagnosis of adrenoleukodystrophy.
abstract: We studied the very-long-chain fatty acids of blood plasma, erythrocyte membranes and lymphocytes in 4 adrenoleukodystrophy patients, 5 adrenoleukodystrophy obligate carriers, 12 normal controls and 81 patients with various neurological disorders by high-performance liquid chromatography and compared the reliabilities in the diagnosis of adrenoleukodystrophy of these 3 components of peripheral blood. Of 81 patients with various neurological disorders, 2 myotonic dystrophy and 2 spinocerebellar degeneration patients showed increased ratios of C26:0 to C22:0 in erythrocyte membranes, but not in blood plasma and lymphocytes. None of the 12 normal controls showed increased ratios of C26:0 to C22:0 in erythrocyte membranes, blood plasma and lymphocytes. These results suggest that fatty acid analysis for the diagnosis of adrenoleukodystrophy is more reliable when blood plasma and lymphocytes are used than when erythrocyte membranes are used.
authors.affiliation: Department of Neurology, National Chikugo Hospital, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Antoku
authors.forename: Y
authors.initials: Y
authors.lastname: Ohtsuka
authors.forename: H
authors.initials: H
authors.lastname: Nagara
authors.forename: T
authors.initials: T
authors.lastname: Sakai
authors.forename: K
authors.initials: K
authors.lastname: Tsukamoto
authors.forename: H
authors.initials: H
authors.lastname: Iwashita
authors.forename: I
authors.initials: I
authors.lastname: Goto
mesh_terms: D000293:Adolescent; D000326:Adrenoleukodystrophy / Q000175:diagnosis* / Q000378:metabolism; D000328:Adult; D002648:Child; D002549:Diffuse Cerebral Sclerosis of Schilder / Q000175:diagnosis*; D004912:Erythrocytes / Q000378:metabolism* / Q000473:pathology; D005227:Fatty Acids / Q000032:analysis*; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism* / Q000473:pathology; D008297:Male; D010949:Plasma / Q000378:metabolism*
chemical_list: D005227:Fatty Acids