Evidence of delayed mesangial transport of human IgA in glomeruli of ddY mice pretreated with sheep anti-type IV collagen serum.
In order to investigate whether mesangial transport by glomeruli is delayed in ddY mice pretreated with sheep anti-type IV collagen serum, the mice were administered an overload of human IgA myeloma serum. Non-pretreated ddY mice used as controls and both experimental and control BALB/c mice were also processed in a similar manner. The intensities of mesangial deposition of human IgA were examined periodically and were found to correlate well with deposition of mouse IgA. Both mouse and human IgAs showed a gradual increase for up to 8 experimental weeks. In the control young ddY mice, however, the overloaded mesangial human IgA quickly disappeared, presenting no appreciable mesangial deposition of autologous IgA. In sharp contrast, both the experimental and control BALB/c mice showed an initially prolonged and rather heavy mesangial deposition of human IgA, followed by a gradual decrease and somewhat light mesangial deposition of autologous mouse IgA. These results obtained using experimental ddY mice appear to confirm the possibility that non-immunological local trapping, due to retardation of mesangial transport function, causes mesangial deposition of autologous mouse IgA in this particular strain.
Masuda
Y
Y
Department of Pathology, Nippon Medical School, Tokyo, Japan.
Ishizaki
M
M
Yamanaka
N
N
Sugisaki
Y
Y
Masugi
Y
Y
D000818:Animals; D001692:Biological Transport; D003094:Collagen / Q000145:classification / Q000276:immunology*; D003176:Complement C3 / Q000378:metabolism; D005920:Glomerular Mesangium / Q000378:metabolism*; D015432:Glomerulonephritis, Membranoproliferative / Q000139:chemically induced / Q000473:pathology; D006801:Humans; D007106:Immune Sera / Q000502:physiology*; D007070:Immunoglobulin A / Q000378:metabolism*; D007075:Immunoglobulin M / Q000378:metabolism; D007136:Immunoglobulins / Q000494:pharmacology; D007668:Kidney / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008817:Mice, Mutant Strains / Q000097:blood / Q000378:metabolism*; D008970:Molecular Weight; D009194:Myeloma Proteins / Q000494:pharmacology; D012756:Sheep
D003176:Complement C3; D007106:Immune Sera; D007070:Immunoglobulin A; D007075:Immunoglobulin M; D007136:Immunoglobulins; D009194:Myeloma Proteins; C017551:myeloma immunoglobulins; D003094:Collagen