Pharmacokinetics of metapramine and its demethylated metabolites in plasma and brain of mice.
Previous studies on pharmacokinetic parameters of tricyclic antidepressants (TCAs) in rodents have shown different results from those obtained for the same drugs in man. The kinetics of metapramine (META) and its major demethylated metabolites (METs) were studied in the SWISS CD 1 mouse after acute administration in order to establish the pharmacokinetic parameters in plasma and brain. The plasma half-life (T1/2) was very short (87 min) compared with the half-life (7 h) in man. The metabolism of META was intensive as was the transfer of META and its metabolites into the brain. The kinetic profiles of the substances were quite similar both in plasma and in brain, namely a bicompartment open model. META was rapidly absorbed (Tmax = 10 min) into and quickly eliminated (T 1/2 = 40 min) from the brain. These parameters were used to schedule sampling (blood and brain) at the appropriate time after acute administration of increased doses. The administered doses were significantly correlated to firstly the plasma or brain levels of META, secondly the plasma levels of the main monodemethylated metabolite (MET I), and thirdly the plasma or brain levels of META + METs. Finally, the evolution of plasma and brain levels of the substances was studied after repeated injections (i.e. every 40 min) and confirmed the high affinity of META and its metabolites for the brain regions.
Bougerolle
A M
AM
Groupe de Recherches en Biodynamique du Médicament, Laboratoire de Chimie Analytique, Clermont-Ferrand, France.
Chabard
J L
JL
Eschalier
A
A
Aumaitre
O
O
Fialip
J
J
Dordain
G
G
Petit
J
J
Berger
J A
JA
D000818:Animals; D000929:Antidepressive Agents, Tricyclic / Q000097:blood / Q000493:pharmacokinetics*; D001921:Brain / Q000378:metabolism*; D003640:Dealkylation; D003984:Dibenzazepines / Q000097:blood / Q000493:pharmacokinetics*; D006207:Half-Life; D008297:Male; D051379:Mice
D000929:Antidepressive Agents, Tricyclic; D003984:Dibenzazepines; C012677:metapramine