title: Differential expression of membrane sialoglycoproteins in exudate and resident mouse peritoneal macrophages.
abstract: Wheat germ agglutinin (WGA) has been used to define biochemical differences between exudate and resident macrophages in the peritoneal cavity of mice. Western blotting with 125I-WGA identifies a restricted set of glycoproteins in elicited peritoneal macrophages (M phi) (recruited with thioglycollate or periodate) and fully activated M phi (recruited with live Bacille Calmette-Guérin, or heat-killed Corynebacterium parvum); the major species migrates with Mr 89-115 kDa in 10% acrylamide gels. These glycoproteins are not detected in resident peritoneal M phi, nor in thymocytes, neutrophils, lymphocytes and a variety of non-M phi cell lines. The binding of WGA is sensitive to neuraminidase, which exposes binding sites on these proteins for peanut agglutinin and reduces their electrophoretic mobility; these features are typical of O-linked sialo-oligosaccharides. In culture, exudate M phi increase their WGA-binding content over 48 h, and continue to display a phenotype distinct from that of resident peritoneal M phi. The stable differential expression of these sialoglycoproteins, by elicited and activated versus resident peritoneal M phi, suggests that biochemical modification during the synthesis and expression of membrane glycoproteins accompanies M phi recruitment to an inflammatory focus.
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, UK.
authors.forename: S
authors.initials: S
authors.lastname: Rabinowitz
authors.forename: S
authors.initials: S
authors.lastname: Gordon
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D005122:Exudates and Transudates / Q000166:cytology; D037102:Lectins / Q000378:metabolism; D008264:Macrophages / Q000032:analysis* / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000032:analysis*; D051379:Mice; D008815:Mice, Inbred Strains; D009439:Neuraminidase / Q000378:metabolism; D010529:Peritoneal Cavity / Q000166:cytology; D012795:Sialoglycoproteins / Q000032:analysis*; D014909:Wheat Germ Agglutinins / Q000378:metabolism
chemical_list: D037102:Lectins; D008562:Membrane Glycoproteins; D012795:Sialoglycoproteins; D014909:Wheat Germ Agglutinins; D009439:Neuraminidase