Auxotrophic mutants of the yeast Trichosporon adeninovorans.
We have isolated and characterized auxotrophic mutants of Trichosporon adeninovorans, strain PAR-4 to get genetic markers that cover the entire nuclear genome of this thermotolerant yeast of technological interest. The nitrosoguanidine mutagenesis yielded mutants at a high frequency. We detected a broad spectrum of auxotrophic phenotypes in the random mutant samples. Obviously, strain PAR-4 is a haploid or hyperhaploid yeast. In correspondence we determined a low DNA content per cell. In contrast to NG1), UV light was an inefficient mutagen. UV survival curves were without the typical shoulder indicating suppression of repair of UV-induced lethal lesions. Thus, the response of PAR-4 to UV was different from those of Saccharomyces cerevisiae and other yeasts.
Samsonova
I A
IA
Ernst-Moritz-Arndt-Universität Greifswald, Sektion Biologie.
Böttcher
F
F
Werner
C
C
Bode
R
R
D004307:Dose-Response Relationship, Radiation; D005819:Genetic Markers; D005838:Genotype; D006238:Haploidy; D003904:Mitosporic Fungi / Q000235:genetics*; D009154:Mutation; D010641:Phenotype; D014250:Trichosporon / Q000235:genetics* / Q000528:radiation effects; D014466:Ultraviolet Rays
D005819:Genetic Markers