Net appearance of amino acids in portal blood during the digestion of casein or rapeseed proteins in the pig.
The kinetics of appearance of amino acids (AA) in portal blood following the ingestion of casein or rapeseed protein were compared. Six pigs, fitted with permanent catheters in the portal vein and in the carotid artery, as well as with an electromagnetic flow probe around the portal vein, received three 800 g test meals, one containing 12% rapeseed proteins (RA12) and the others containing 12% and 24% casein (CA12 and CA24), at 1-week intervals and according to a double Latin square design. Portal and arterial blood samples were collected and portal blood flow rate was recorded for 8 h after the test meals. At the end of measurement, an average of 76.1 +/- 5.6% (mean +/- SEM) of total AA from the CA24 diet had appeared in portal blood, compared with 94.3 +/- 10.4% for the CA12 diet and 103.5 +/- 12.6% for the RA12 diet. Similar results were obtained for essential AA. Differences were found in the kinetics of appearance of individual AA. Eight hours after the meal, 79% of lysine, 84% of methionine, and 73% of valine from the CA24 diet had appeared in portal blood compared, respectively, with 100, 89, and 83% from the CA12 diet and 99, 86, and 106% from the RA12 diet. Arginine from rapeseed had a net appearance level lower (82%) than the overall mixture of essential AA. With casein diets, the net appearance of arginine reached 97% (CA12) and 82% (CA24). Following the ingestion of rapeseed proteins, there seemed to be a significant appearance of endogenous AA in portal blood.(ABSTRACT TRUNCATED AT 250 WORDS)
Galibois
I
I
Département de nutrition humaine et de consommation, Université Laval, Sainte-Foy (Qué.), Canada.
Simoes Nunes
C
C
Rérat
A
A
Savoie
L
L
D000596:Amino Acids / Q000032:analysis / Q000097:blood*; D000601:Amino Acids, Essential / Q000097:blood; D000818:Animals; D001937:Brassica / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000097:blood; D002364:Caseins / Q000378:metabolism*; D004032:Diet; D008297:Male; D009584:Nitrogen / Q000097:blood; D010940:Plant Proteins / Q000378:metabolism*; D011169:Portal Vein / Q000378:metabolism; D013552:Swine
D000596:Amino Acids; D000601:Amino Acids, Essential; D002241:Carbohydrates; D002364:Caseins; D010940:Plant Proteins; D009584:Nitrogen