Monoclonal antibodies against IgG allotypes G1m(z), G1m(a), G1m(f), G3m(b1/u) and G3m(g1): their usefulness in HAI and capture ELISA.
Monoclonal antibodies (McAbs) were produced against the IgG allotypes G1m(z), G1m(a), G1m(f), G3m(b1/u) and G3m(g1). Four out of the six McAbs described in this paper showed in the haemagglutination assay cross-reactivity with some or all IgG-coated cell samples. In the haemagglutination inhibition assay, all six McAbs are useful as typing reagent for the above allotypes. In this assay, two of the McAbs show two different specificities, which depend on the Ig-coated cell sample used. Five McAbs are useful for allotyping in a capture ELISA. The results with four of these are promising for the development of a quantitative determination of Gm allotypes.
de Lange
G G
GG
Central Laboratory, The Netherlands Red Cross Blood Transfusion Service.
van Leeuwen
A M
AM
Vlug
A
A
van Eede
P H
PH
Engelfriet
C P
CP
Lincoln
P J
PJ
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001788:Blood Grouping and Crossmatching / Q000379:methods; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005819:Genetic Markers; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007129:Immunoglobulin Gm Allotypes / Q000032:analysis*
D000911:Antibodies, Monoclonal; D005819:Genetic Markers; D007129:Immunoglobulin Gm Allotypes