The use of porcine hepatocytes for biotransformation studies of veterinary drugs.
1. Cultures of porcine hepatocytes with high viability were isolated from a liver sample by a simple procedure. In ageing monolayer cultures the cytochrome P-450 content and 7-ethoxycoumarin-O-deethylase activity decreased gradually while glutathione levels increased. 2. The nitrofuran, furazolidone, was rapidly metabolized, partly resulting in the formation of 3-(4-cyano-2-oxobutylidene amino)-2-oxazolidone. 3. Acetylating and deacetylating activities towards sulphadimidine and its N4-acetyl metabolite were present in porcine hepatocytes. Relative and absolute levels of these activities varied in different batches of hepatocytes. 4. No differences were seen in a number of enzyme activities measured in cytosolic and microsomal fractions isolated from different lobes of one liver. Differences between livers from different animals were marked.
Hoogenboom
L A
LA
State Institute for Quality Control of Agricultural Products (RIKILT), Wageningen, The Netherlands.
Pastoor
F J
FJ
Clous
W E
WE
Hesse
S E
SE
Kuiper
H A
HA
D015660:7-Alkoxycoumarin O-Dealkylase / Q000032:analysis / Q000378:metabolism; D000818:Animals; D001711:Biotransformation; D002478:Cells, Cultured; D003374:Coumarins / Q000493:pharmacokinetics*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005260:Female; D005664:Furazolidone / Q000493:pharmacokinetics*; D005978:Glutathione / Q000032:analysis / Q000378:metabolism; D006651:Histocytochemistry; D007700:Kinetics; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008954:Models, Biological; D011247:Pregnancy; D013418:Sulfamethazine / Q000493:pharmacokinetics*; D013552:Swine
D003374:Coumarins; C017299:7-ethoxycoumarin; D013418:Sulfamethazine; D005664:Furazolidone; D003577:Cytochrome P-450 Enzyme System; D015660:7-Alkoxycoumarin O-Dealkylase; D005978:Glutathione