Two-dimensional electrophoresis aided by personal computer analysis for screening of mutant proteins in inherited diseases.
A rapid and reproducible method of two-dimensional electrophoresis was developed for screening of abnormal proteins expressed in fibroblasts from patients with inherited diseases. After silver staining, the electrophoresis gel was subjected to semiautomatic digitizer-personal computer analysis: scanning with an image sensor video camera connected to a digitizer, followed by quantitative determination and statistical analysis with a personal computer. The protein spots analyzed by this method showed quantitative variations of various degrees, particularly in 2 of 247 spots examined. Seven spots were not always detected in control and pathological cells in this study. Slight variations in molecular weight were observed in 3 different spots.
Yang
R C
RC
Department of Clinical Genetics, Tokyo Metropolitan Institute of Medical Science, Japan.
Tsuji
A
A
Suzuki
Y
Y
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D001330:Electronic Data Processing; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D030342:Genetic Diseases, Inborn / Q000209:etiology*; D006801:Humans; D007223:Infant; D008297:Male; D009154:Mutation; D011506:Proteins / Q000235:genetics; D012737:Sex Factors
D011506:Proteins