title: Serum lipoproteins are required for multiplication of Trypanosoma brucei brucei under axenic culture conditions.
abstract: Bloodstream form Trypanosoma brucei brucei IL3201 and IL3202, derived from two different serodemes, were adapted to grow in a semi-defined medium under axenic culture conditions. The organisms required low- or high-density lipoproteins, isolated from foetal bovine serum (FBS) in addition to components of lipoprotein-depleted serum, to multiply in vitro. Low- and high-density lipoproteins, isolated from a number of different species, were equally efficient at supporting in vitro growth of the parasites in medium supplemented with lipoprotein-depleted FBS. Chylomicrons and very-low-density lipoproteins did not support multiplication of the T. b. brucei and did not influence the capacity of low- or high-density lipoproteins to support parasite multiplication. Removal of the lipoprotein-lipids abrogated the capacity of low- or high-density lipoproteins to support T. b. brucei multiplication.
authors.affiliation: International Laboratory for Research on Animal Diseases, Nairobi, Kenya.
authors.forename: S
authors.initials: S
authors.lastname: Black
authors.forename: V
authors.initials: V
authors.lastname: Vandeweerd
mesh_terms: D000818:Animals; D001053:Apolipoproteins / Q000502:physiology; D002417:Cattle; D008074:Lipoproteins / Q000502:physiology*; D008075:Lipoproteins, HDL / Q000502:physiology; D008077:Lipoproteins, LDL / Q000502:physiology; D051379:Mice; D011817:Rabbits; D051381:Rats; D014346:Trypanosoma brucei brucei / Q000254:growth & development*
chemical_list: D001053:Apolipoproteins; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL