title: Morphologic observations of retinal pigment epithelial proliferation and neovascularization in the rabbit.
abstract: Neovascularization and proliferation of the retinal pigment epithelium (RPE) was induced in the rabbit by subretinal injection of vitreous without rupture of Bruch's membrane. New vessels developed between the layer of RPE and photoreceptor outer segments, but were enveloped in proliferating RPE. For this reason they were occult; no fluorescein leakage was visible by angiography. The vessels were identified only by histologic examinations. Endothelial cell budding was the initial stage of vessel development, first seen two weeks after injection. The new vessels grew from the choriocapillaris, penetrated Bruch's membrane, and spread into the subretinal space, despite the absence of subretinal fluid. Fenestrations with diaphragms were found in the endothelial walls during the earliest stages of vessel formation, and were also present in the fully matured vessels. Intermediate junctional complexes were frequently observed among the endothelial cells. During maturation of these plexi, junctions changed from open to putative tight junctions. The mature vessels were ultimately completely enveloped by collagen and RPE cells. Our results show that all new vessels in this animal model have the morphologic characteristics of choriocapillaris. We assume that they leak fluorescein, as does the choriocapillaris, but that the dye has no opportunity to pool in the subretinal space and thus cannot be seen during angiography.
authors.affiliation: Department of Ophthamology, University of Southern California, Los Angeles 90033.
authors.forename: Z R
authors.initials: ZR
authors.lastname: Zhu
authors.forename: R
authors.initials: R
authors.lastname: Goodnight
authors.forename: N
authors.initials: N
authors.lastname: Sorgente
authors.forename: T E
authors.initials: TE
authors.lastname: Ogden
authors.forename: S J
authors.initials: SJ
authors.lastname: Ryan
mesh_terms: D000818:Animals; D002455:Cell Division; D004195:Disease Models, Animal; D004730:Endothelium, Vascular / Q000648:ultrastructure; D005451:Fluorescein Angiography; D010857:Pigment Epithelium of Eye / Q000473:pathology*; D011817:Rabbits; D015861:Retinal Neovascularization / Q000473:pathology*; D012171:Retinal Vessels / Q000648:ultrastructure