Immunohistochemical localization of type IV collagen fibronectin and laminin in the juxtaglomerular apparatus of the rat kidney.
The juxtaglomerular apparatus (JGA) is a complex structure containing several components: the vessels, the extraglomerular mesangium and the distal tubule. These structures include cellular elements and an extracellular matrix (ECM). Collagenous (type IV collagen) and noncollagenous components of the basement membranes were studied. The localization of type IV collagen and of two extracellular glycoproteins (laminin and fibronectin) was investigated using immunofluorescent and immunoperoxidase labelled antibodies. Type IV collagen and laminin have the same localization on the JGA basement membranes. On the other hand, fibronectin is limited to the entrance of the glomerular stalk. On electron microscopy, type IV collagen is found in the basement membrane while fibronectin is restricted to certain areas of the extracellular matrix. These findings confirm data concerning the distribution of these three components in basement membranes and allow a better understanding of the histoarchitecture of the juxtaglomerular apparatus.
Blanc-Brunat
N
N
Mutin
M
M
Peyrol
S
S
D000818:Animals; D003094:Collagen / Q000032:analysis*; D005353:Fibronectins / Q000032:analysis*; D007150:Immunohistochemistry; D007606:Juxtaglomerular Apparatus / Q000032:analysis* / Q000648:ultrastructure; D007797:Laminin / Q000032:analysis*; D051381:Rats
D005353:Fibronectins; D007797:Laminin; D003094:Collagen