Effect of dexamethasone on arachidonate metabolism in isolated mouse thymocytes.
In order to investigate the effects of glucocorticoids on arachidonate release and metabolism in mouse thymocytes, we have studied both the action of dexamethasone on arachidonate release from pre-labeled cells and its effect on tracer uptake and metabolism. Our results indicate, first, that dexamethasone failed in this experimental model to affect phospholipase activity; second, that glucocorticoids are able to inhibit the transformation of the precursor into prostaglandins and to block simultaneously its acylation into phospholipids; finally that isolated thymocytes secrete significant amounts of 12-HETE, and that this secretion is unaffected by steroid treatment.
Duval
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Inserm U7, Hôpital Necker, Paris, France.
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism*; D002845:Chromatography; D003907:Dexamethasone / Q000494:pharmacology*; D005260:Female; D066298:In Vitro Techniques; D007700:Kinetics; D051379:Mice; D008810:Mice, Inbred C57BL; D011453:Prostaglandins / Q000097:blood; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*
D001095:Arachidonic Acids; D011453:Prostaglandins; D003907:Dexamethasone