Identification and primary structure of the gene encoding the Berne virus nucleocapsid protein.
The nucleotide sequence of the nucleocapsid (N) protein gene of Berne virus (BEV; proposed family Toroviridae) was determined from two independent clones of a cDNA library. From the deduced amino acid sequence a basic protein of 18.3K was predicted. In vitro transcription and translation, followed by immunoprecipitation, were used to identify the gene. The identification was confirmed by metabolic labelling, using the knowledge that cysteine residues are absent from the amino acid sequence of the N protein. Smaller N-related polypeptides encountered in BEV-infected cell lysates were shown to be probable products of aberrant translation, due to initiation on AUG codons further downstream in the N protein gene.
Snijder
E J
EJ
Institute of Virology, Veterinary Faculty, State University of Utrecht, The Netherlands.
den Boon
J A
JA
Spaan
W J
WJ
Verjans
G M
GM
Horzinek
M C
MC
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D012328:RNA Viruses / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014758:Viral Core Proteins / Q000235:genetics*
D012367:RNA, Viral; D014758:Viral Core Proteins