title: In vitro induction of a diphtheria toxoid specific antibody response in human peripheral blood lymphocytes cultivated in the presence of diphtheria toxoid.
abstract: In comparison with the presently used potency test for diphtheria vaccine, in vitro examination of the immunogenicity of the vaccine would have great advantages. For this reason in vitro induction of diphtheria toxoid specific antibody synthesis in human peripheral blood lymphocytes cultivated in the presence of diphtheria toxoid was investigated. The results showed that a dose dependent synthesis of diphtheria antibody was induced by adsorbed diphtheria toxoid and combined vaccines containing the diphtheria toxoid component. Plain diphtheria toxoid appeared to be less immunogenic in comparison with adsorbed toxoid. There is some indication that the pertussis component had a stimulating effect on the diphtheria antibody synthesis. In conclusion, these results are promising for in vitro examination of the immunogenicity of diphtheria vaccines. The model will be validated for the routine control of diphtheria vaccine.
authors.affiliation: National Institute of Public Health and Environmental Protection Laboratory for Control of Bacterial Vaccines, Bilthoven, The Netherlands.
authors.forename: H G
authors.initials: HG
authors.lastname: Loggen
authors.forename: J G
authors.initials: JG
authors.lastname: Kreeftenberg
mesh_terms: D000017:ABO Blood-Group System; D000276:Adjuvants, Immunologic; D000327:Adsorption; D000535:Aluminum; D017607:Aluminum Compounds; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D001782:Blood Donors; D002478:Cells, Cultured; D004166:Diphtheria Antitoxin / Q000032:analysis / Q000096:biosynthesis; D004168:Diphtheria Toxoid / Q000276:immunology*; D022422:Diphtheria-Tetanus Vaccine; D015721:Diphtheria-Tetanus-Pertussis Vaccine / Q000276:immunology; D004338:Drug Combinations; D004797:Enzyme-Linked Immunosorbent Assay; D006736:Horses; D006801:Humans; D008214:Lymphocytes / Q000276:immunology*; D010710:Phosphates; D011054:Poliovirus Vaccine, Inactivated / Q000276:immunology; D013745:Tetanus Toxoid
chemical_list: D000017:ABO Blood-Group System; D000276:Adjuvants, Immunologic; D017607:Aluminum Compounds; D000907:Antibodies, Bacterial; D004166:Diphtheria Antitoxin; D004168:Diphtheria Toxoid; D022422:Diphtheria-Tetanus Vaccine; D015721:Diphtheria-Tetanus-Pertussis Vaccine; D004338:Drug Combinations; D010710:Phosphates; D011054:Poliovirus Vaccine, Inactivated; D013745:Tetanus Toxoid; D000535:Aluminum; C012714:aluminum phosphate