title: [Isolation and purification of biopolymers using an affinity chromatography method. X. A biospecific approach to preparing collagen-like peptides containing segments binding fibronectin].
abstract: A biospecific method for one-stage isolation of collagen peptides containing the fibronectin binding site is proposed. alpha 1CB7-peptide of the type I collagen cyanogen bromide cleavage was isolated by means of affinity chromatography on adsorbents containing an immobilized gelatin-binding domain (45 kDa) of fibronectin. The method allows one to obtain, in a short time, highly purified preparation of alpha 1CB7-peptide necessary for biochemical studies.
authors.lastname: Mitina VKhA
authors.forename: N A
authors.initials: NA
authors.lastname: Frantsuzova
authors.forename: V A
authors.initials: VA
authors.lastname: Kliashchitskiĭ
mesh_terms: D001704:Biopolymers; D002352:Carrier Proteins; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D046911:Macromolecular Substances; D011506:Proteins / Q000302:isolation & purification*
chemical_list: D001704:Biopolymers; D002352:Carrier Proteins; D005353:Fibronectins; D046911:Macromolecular Substances; D011506:Proteins; C007105:c1q-binding proteins
vernacular_title: Vydelenie i ochistka biopolimerov metodom affinnoĭ khromatografii. X. Biospetsificheskiĭ podkhod k polucheniiu kollagenovykh peptidov, soderzhashchikh uchastok sviazyvaniia s fibronektinom.