Factors affecting aerobic recovery heat production and recovery ratio of frog sartorius.
1. Sartorius muscles of Rana temporaria, equilibrated at 20 degrees C in Ringer solution buffered with phosphates, were stimulated isometrically for 0.2 up to 0.75 s at lengths varying from 1.03 to 1.48 times rest length, L0. The aerobic recovery heat was measured for 10.5 min after contraction. 2. The recovery heat production had a complex time course, showing a variable delay to maximum, declining thereafter. In most cases, the rate of heat production did not decrease monotonically; attention was focused on the slow exponential decay which only persisted from 1.5-5 min after contraction. This latter part of the time course was considered as strictly aerobic and characterized by the time constant tau s. 3. Increasing the tetanus duration from 0.2 to 0.75 s increased initial heat Qi and recovery heat Qr in proportion, so that the recovery ratio R (Qr/Qi) did not change; it was equal to 1.29 +/- 0.03 (S.E.M.; n = 44) for muscles at about L0. The kinetics of heat production were modified with longer tetani; in particular, tau s was increased from 2.2 to 5.2 min. 4. When muscles were stretched beyond L0, as long as there was no increase of the resting heat rate (stretch response or 'Feng effect'), recovery heat production had a similar evolution to that in muscles at about L0; R was constant and equal to 1.21 +/- 0.03 (n = 46). 5. When muscles were sufficiently stretched to develop a stretch response, R increased proportionally to the stretch response. The effect seemed independent of the contractile machinery, as it vanished concomitantly with the stretch response, while force and Qi remained unchanged for the length considered. The kinetics were also modified--the delay to maximum was no longer detected and tau s most likely increased. 6. Substitution of 60% of the NaCl of the Ringer solution by NaI (mol/mol) produced a significant increase of R, mainly due to the increase of Qr. 7. The results show that neither the time course nor the amount of aerobic recovery heat Qr are strictly determined by the amount of initial heat Qi. The hypothesis is discussed that Qr might include a variable fraction due to processes which are not directly implicated in the actin-myosin interactions, possibly those involving the cytosolic Ca2+ concentration and the rate of resting metabolism.
Godfraind-De Becker
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Department of Physiology, Université Catholique de Louvain, Brussels, Belgium.
D000818:Animals; D001833:Body Temperature Regulation / Q000502:physiology*; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000502:physiology*; D009132:Muscles / Q000378:metabolism*; D011896:Rana temporaria