title: The influence of subminimal inhibitory concentrations of netilmicin and ceftriaxone on the interaction of Escherichia coli with host defences.
abstract: The effect of sub-MICs of netilmicin and ceftriaxone on the interaction between encapsulated and unencapsulated strains of Escherichia coli and certain host defence mechanisms, i.e. complement activation, opsonization, phagocytosis by human polymorphonuclear leucocytes (PMN), and serum bactericidal activity have been studied. Experiments were carried out testing antibiotics either alone or in combination. Non-capsulated strains of E. coli activated complement rapidly and were easily phagocytosed and killed after opsonization in human pooled serum. Pretreatment of these strains with sub-MICs of antibiotics did not change the rate of opsonization or the degree of uptake by PMN, but did enhance serum sensitivity. Capsulated strains of E. coli were both poorly opsonized and resistant to serum bactericidal activity. Treatment of these strains with sub-MICs of antibiotics enhanced complement consumption as well as phagocytosis by PMN, but did not affect serum-resistance.
authors.affiliation: Department of Clinical Microbiology, University of Utrecht, The Netherlands.
authors.forename: G
authors.initials: G
authors.lastname: Raponi
authors.forename: R W
authors.initials: RW
authors.lastname: Vreede
authors.forename: M
authors.initials: M
authors.lastname: Rozenberg-Arska
authors.forename: I M
authors.initials: IM
authors.lastname: Hoepelman
authors.forename: N
authors.initials: N
authors.lastname: Keller
authors.forename: J
authors.initials: J
authors.lastname: Verhoef
mesh_terms: D001770:Blood Bactericidal Activity / Q000187:drug effects; D002443:Ceftriaxone / Q000494:pharmacology*; D003165:Complement System Proteins / Q000276:immunology; D004926:Escherichia coli / Q000187:drug effects*; D004927:Escherichia coli Infections / Q000276:immunology*; D006462:Hemolytic Plaque Technique; D006801:Humans; D066298:In Vitro Techniques; D008826:Microbial Sensitivity Tests; D009428:Netilmicin / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects; D009895:Opsonin Proteins / Q000502:physiology; D010587:Phagocytosis / Q000187:drug effects
chemical_list: D009895:Opsonin Proteins; D009428:Netilmicin; D002443:Ceftriaxone; D003165:Complement System Proteins