title: Purification and properties of RNA polymerase from acidophilic heterotroph SJH.
abstract: RNA polymerase was isolated from the acidophilic heterotrophic bacterium SJH and purified to homogeneity. Subunit structure (beta', beta, theta, alpha 2) resembled that of other gram-negative bacteria such as Escherichia coli. pH optimum of the enzyme was around 8.0, although the bacterium grew only below pH 6.0 and optimally between 3.0-4.0. SJH and E. coli RNA polymerases showed maximal activities on homologous high molecular-weight DNA templates.
authors.affiliation: Department of Biochemistry, University of Sussex, Brighton, U.K.
authors.forename: T J
authors.initials: TJ
authors.lastname: Beebee
mesh_terms: D001419:Bacteria / Q000201:enzymology*; D004269:DNA, Bacterial / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D013698:Templates, Genetic
chemical_list: D004269:DNA, Bacterial; D012321:DNA-Directed RNA Polymerases