[An electron microscopic study of neuronal development in organotypic cultures of the anlage of the cerebral cortex from a human embryo].
Data are provided on cytodifferentiation of the cerebral cortex cultured cells taken from 10-12 week old embryos of man. It is shown that low differentiated neuroblasts well survive in culture for 21 days. Mature granular cells and middle pyramidal neurons are revealed in cultures. The number of morphological criteria may testify to the maturity of neurons: the presence of the Nissl substance, differentiation of dendrites and axons; the presence of various types of synapses. The absence of myelinized fibres testifies to the insufficient maturity of the cultures, that is probably associated with employing the low differentiated nervous tissue for cultivation and with insufficient cultivation period.
Bobryshev
Iu V
IuV
Balabanov
Iu V
IuV
Pavlov
O V
OV
Chumasov
E I
EI
D002454:Cell Differentiation; D002540:Cerebral Cortex / Q000196:embryology / Q000648:ultrastructure*; D006801:Humans; D008854:Microscopy, Electron; D009474:Neurons / Q000648:ultrastructure*; D009924:Organ Culture Techniques / Q000379:methods; D013997:Time Factors
Elektronno-mikroskopicheskoe issledovanie razvitiia neĭronov v organotipicheskikh kul'turakh zakladki kory golovnogo mozga émbriona cheloveka.