[Histological evaluation of tooth movement using immunocytochemical research].
A new immunocytochemical methodology has been applied to value the proliferation in the periodontal tissue following experimental tooth movement. Orthodontic tooth movement has been obtained in Guinea pigs by a coil sporing, and a proliferation marker administrated "in vivo"; the proliferation marker used is Bromodeoxyuridine, a thimidine analogue which is taken up by DNA synthesizing nuclei, and afterwards intensified by two monoclonal antibodies, where the second is FITC coniugated. Instead of some technical difficulties, the results are encouraging for further development.
Sfondrini
G
G
Gandini
P
P
Bovari
M
M
Bottone
M G
MG
D000818:Animals; D000911:Antibodies, Monoclonal; D001862:Bone Resorption / Q000209:etiology / Q000276:immunology / Q000473:pathology*; D001973:Bromodeoxyuridine; D002455:Cell Division; D006168:Guinea Pigs; D007150:Immunohistochemistry; D009967:Orthodontic Appliances / Q000009:adverse effects; D010519:Periodontium / Q000473:pathology*; D014087:Tooth Movement Techniques / Q000009:adverse effects*
D000911:Antibodies, Monoclonal; D001973:Bromodeoxyuridine
Valutazione istologica degli spostamenti dentari mediante indagine immunocitochimica.