title: Crystallization of thermitase, a thermostable subtilisin, from a sodium formate solution by means of an automated procedure.
abstract: A new crystal form of native thermitase has been obtained using sodium formate as the precipitating agent and employing an automated crystallization procedure. The crystals have the form of tetragonal bipyramids, the longest dimension being about 0.4 mm. The space group is P4(1)2(1)2 or P4(3)2(1)2, with a = 182 A and b = c = 53.3 A. The crystals diffract beyond 2.5 A.
authors.affiliation: Laboratory of Chemical Physics, University of Groningen, The Netherlands.
authors.forename: H A
authors.initials: HA
authors.lastname: Kelders
authors.forename: K H
authors.initials: KH
authors.lastname: Kalk
authors.forename: W G
authors.initials: WG
authors.lastname: Hol
mesh_terms: D003460:Crystallization; D010450:Endopeptidases; D005561:Formates; D006358:Hot Temperature; D008722:Methods; D012697:Serine Endopeptidases
chemical_list: D005561:Formates; C030544:formic acid; D010450:Endopeptidases; D012697:Serine Endopeptidases; C022728:thermitase