Structural studies on the fucosamine-containing O-specific polysaccharide of Proteus vulgaris O19.
The polysaccharide chain of Proteus vulgaris O19 lipopolysaccharide contains D-galactose, N-acetyl-D-glucosamine N-acetyl-D-galactosamine and N-acetyl-L-fucosamine in the ratio 1:1:1:1. The structure of the polysaccharide was established by full acid hydrolysis and methylation analysis, as well as by non-destructive methods, i.e. the computer-assisted evaluation of the 13C-NMR spectrum and computer-assisted evaluation of the specific optical rotation by Klyne's rule. The polysaccharide is regular and built up of tetrasaccharide repeating units of the following structure: ----3)-alpha-L-FucNAcp-(1----3)-beta-D-GlcNAcp-(1----3)-alph a-D-Galp- (1----4)-alpha-D-GalNAcp-(1---- The O19-antiserum cross-reacts with lipopolysaccharide from P. vulgaris O42, the structure of which is still unknown. No cross-reactions were observed with O-polysaccharides Pseudomonas aeruginosa O7 and Salmonella arizonae O59 in spite of some structural similarities.
Vinogradov
E V
EV
N. D. Zelinsky Institute of Organic Chemistry, Academy of Sciences of the USSR, Moscow.
Kaca
W
W
Knirel
Y A
YA
Rózalski
A
A
Kochetkov
N K
NK
D000116:Acetylgalactosamine / Q000032:analysis*; D000117:Acetylglucosamine / Q000032:analysis*; D015153:Blotting, Western; D002236:Carbohydrate Conformation; D004591:Electrophoresis, Polyacrylamide Gel; D005643:Fucose / Q000031:analogs & derivatives* / Q000032:analysis / Q000276:immunology; D005688:Galactosamine / Q000031:analogs & derivatives*; D005690:Galactose / Q000032:analysis*; D005944:Glucosamine / Q000031:analogs & derivatives*; D006868:Hydrolysis; D007106:Immune Sera; D008070:Lipopolysaccharides / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D015394:Molecular Structure; D009904:Optical Rotation; D011233:Precipitin Tests; D011514:Proteus vulgaris / Q000032:analysis*
D007106:Immune Sera; D008070:Lipopolysaccharides; D005643:Fucose; C059377:N-acetylfucosamine; D005688:Galactosamine; D000116:Acetylgalactosamine; D005944:Glucosamine; D000117:Acetylglucosamine; D005690:Galactose