Escherichia coli dnaT gene function is required for pBR322 plasmid replication but not for R1 plasmid replication.
Plasmid pBR322 was unable to replicate in a temperature-sensitive dnaT1 strain at a nonpermissive temperature, whereas a pBR322-derived plasmid carrying the wild-type dnaT+ gene was able to replicate under the same conditions. In contrast to pBR322, plasmid R1 could replicate in the dnaT1 strain at a nonpermissive temperature. In keeping with this finding, in vitro replication of plasmid R1 did not require DnaT protein.
Masai
H
H
Department of Molecular Biology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, California 94304.
Arai
K
K
D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D066298:In Vitro Techniques; D009154:Mutation; D010957:Plasmids; D012045:Regulatory Sequences, Nucleic Acid; D013696:Temperature
D004269:DNA, Bacterial