Characterization of opiate binding sites on membranes of rat lymphocytes.
In view of the importance of membrane receptors for the interconnection between the central nervous system and the immune system, we carried out a study to characterize opiate binding sites on membranes of rat lymphocytes. We found that mitogen-activated spleen cells, but not thymocytes, possess specific and displaceable binding sites for [3H]naloxone. The binding was equally effective and intense while using B-cell-depleted spleen cells. The binding showed two sites of saturation, one at 10 nM and the other at concentrations greater than 20 nM of [3H]naloxone. Computer analysis of the binding data obtained with the lowest concentrations of naloxone revealed a unique site with high affinity binding to opiates. Displacement was achieved with morphine sulphate and naloxone but not with opioid peptides. The binding of the antagonist, [3H]naloxone, was profoundly inhibited by the co-presence of 120 mM NaCl and up to 100 microM guanosine 5'-O-(3-thiotriphosphate (GTP gamma S). Other metal ions and cyclic nucleotides were not able to interfere with the specific binding. This specificity for GTP analogues is consistent with the hypothesis that a GTP-binding regulatory protein that couples receptors to adenylate cyclase is involved in the process of binding of opiates to lymphocytes. The existence of binding sites on lymphoid cells, analogous to receptors for agents known to affect brain functions, may be another link between the immune system and the central nervous system.
Ovadia
H
H
Department of Neurology, Hadassah Hebrew University Hospital, Jerusalem, Israel.
Nitsan
P
P
Abramsky
O
O
D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002462:Cell Membrane / Q000378:metabolism; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D008214:Lymphocytes / Q000378:metabolism*; D009020:Morphine / Q000378:metabolism; D009270:Naloxone / Q000378:metabolism; D009294:Narcotics / Q000378:metabolism*; D051381:Rats; D012964:Sodium / Q000494:pharmacology; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D013873:Thionucleotides / Q000494:pharmacology; D013950:Thymus Gland / Q000166:cytology / Q000378:metabolism
D009294:Narcotics; D013873:Thionucleotides; D009270:Naloxone; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D009020:Morphine; D006160:Guanosine Triphosphate; D012964:Sodium