Production of mammastatin, a tissue-specific growth inhibitor, by normal human mammary cells.
The growth of human mammary cells may be regulated by a balance between growth stimulatory and growth inhibitory pathways. Polypeptides of 47 and 65 kilodaltons (mammastatin) were isolated from conditioned medium of normal human mammary cells. Monoclonal antibodies against mammastatin were generated that blocked its activity and were used for purification and further characterization of the protein. Mammastatin inhibited the growth of 5 transformed human mammary cell lines, but had no effect on the growth of 11 transformed human cell lines derived from nonmammary tissues. Mammastatin appeared to be a heat-labile protein distinct from transforming growth factor-beta (TGF-beta). By immunoperoxidase staining it was detected in cultured normal human mammary cells, but was decreased in transformed mammary cells.
Ervin
P R
PR
Department of Internal Medicine, University of Michigan Cancer Center, Ann Arbor 48109.
Kaminski
M S
MS
Cody
R L
RL
Wicha
M S
MS
D000911:Antibodies, Monoclonal; D001940:Breast / Q000032:analysis / Q000166:cytology / Q000378:metabolism*; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology; D002455:Cell Division; D002461:Cell Line, Transformed; D002845:Chromatography; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004848:Epithelium / Q000378:metabolism; D005260:Female; D006131:Growth Inhibitors / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D006358:Hot Temperature; D006801:Humans; D007118:Immunoassay; D007124:Immunoenzyme Techniques; D036341:Intercellular Signaling Peptides and Proteins; D008970:Molecular Weight; D010452:Peptide Biosynthesis; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D006131:Growth Inhibitors; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; C060120:mammastatin; D014357:Trypsin