title: Axial diffusion of retinol in isolated frog rod outer segments following substantial bleaches of visual pigment.
abstract: Partial bleaches of rhodopsin were made in either the proximal or distal halves of isolated Rana pipiens rod outer segments. The fluorescence of all-trans retinol was recorded 15, 60 and 120 min following 17% and 63% bleaches. Some of the retinol that formed remained immobilized in the bleached halves of the outer segments, while another portion was slowly mobilized and diffused along the cell axis.
authors.affiliation: R. S. Dow Neurological Sciences Institute, Good Samaritan Hospital and Medical Center, Portland, OR 97209-1595.
authors.forename: R C
authors.initials: RC
authors.lastname: Sears
authors.forename: M W
authors.initials: MW
authors.lastname: Kaplan
mesh_terms: D000818:Animals; D002138:Calibration; D004058:Diffusion; D005453:Fluorescence; D008027:Light; D010786:Photoreceptor Cells / Q000378:metabolism*; D011894:Rana pipiens; D012168:Retinal Pigments / Q000378:metabolism* / Q000502:physiology; D012243:Rhodopsin / Q000378:metabolism; D012374:Rod Cell Outer Segment / Q000378:metabolism*; D014801:Vitamin A / Q000378:metabolism*
chemical_list: D012168:Retinal Pigments; D014801:Vitamin A; D012243:Rhodopsin