Immunohistochemical analysis of cytochrome P-450 17 alpha-hydroxylase in pig adrenal cortex, testis and ovary.
Cytochrome P-450 specific for steroid 17 alpha-hydroxylation (P-450(17 alpha] was immunohistochemically observed in pig adrenal cortex, testis and ovary by the biotin-streptavidin method using a specific antibody against P-450(17 alpha) purified from neonatal pig testis. In the adrenal cortex, P-450(17 alpha) was present in the zona fasciculata and reticularis while no immunoreactivity was observed in the zona glomerulosa, confirming the absence of 17-hydroxylated steroid synthesis in the zona glomerulosa. In the testis, P-450(17 alpha) was present exclusively in Leydig cells and immunoreactivity was absent in seminiferous tubules. In the ovary, immunoreactivity was observed only in the theca interna but not in the membrana granulosa of follicles. Among the tissues examined, the relative intensity of immunoreactivity was greatest in the Leydig cells, and progressively less in theca interna cells, outer fasciculata cells and inner fasciculata and reticularis cells.
Sasano
H
H
Department of Pathology, Tohoku University School of Medicine, Sendai, Japan.
Mason
J I
JI
Sasano
N
N
D000302:Adrenal Cortex / Q000201:enzymology*; D000818:Animals; D005260:Female; D007124:Immunoenzyme Techniques; D008297:Male; D010053:Ovary / Q000201:enzymology*; D013254:Steroid 17-alpha-Hydroxylase / Q000032:analysis*; D013250:Steroid Hydroxylases / Q000032:analysis*; D013552:Swine; D013737:Testis / Q000201:enzymology*
D013250:Steroid Hydroxylases; D013254:Steroid 17-alpha-Hydroxylase