Cloning of the glutamine:fructose-6-phosphate amidotransferase gene from yeast. Pheromonal regulation of its transcription.
The activity of the amino sugar-synthesizing enzyme L-glutamine:D-fructose-6-phosphate aminotransferase (EC 2.6.1.16) in haploid a cells of Saccharomyces cerevisiae increases 1.7-fold after alpha factor addition. The gene (the gene should be called GFA1 for glutamine:fructose-6-phosphate amidotransferase) for the enzyme has been cloned by complementing the gcn1 mutation (Whelan, W. L., and Ballou, C. E. (1975) J. Bacteriol. 125, 1545-1557). Its expression is increased 2-3 times within 15 min when the mating pheromone is added. The gene codes for a protein of 716 amino acids in length. It is highly homologous (64%) to the corresponding gene of Escherichia coli, except for a sequence coding for 83 amino acids (numbers 204-286), which is lacking in E. coli. The amino-terminal region of the coding sequence also shows a high degree of homology to the corresponding sequence of the E. coli and S. cerevisiae L-glutamine:phosphoribosylpyrophosphate amidotransferase. In the promotor region of the S. cerevisiae L-glutamine:D-fructose-6-phosphate amidotransferase gene the heptanucleotide "TGAAACA," shown to be required for pheromone control of transcription (Kronstad, J. W., Holly, J. A., and MacKay, V. L. (1987) Cell 50, 369-377), is present six times.
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Lehrstuhl für Zellbiologie und Pflanzenphysiologie, Universität Regensburg, Federal Republic of Germany.
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D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D005796:Genes; D005800:Genes, Fungal; D005945:Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing) / Q000235:genetics* / Q000378:metabolism; D000072235:Mating Factor; D008969:Molecular Sequence Data; D010455:Peptides / Q000502:physiology*; D010675:Pheromones / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D000637:Transaminases / Q000235:genetics*; D014158:Transcription, Genetic
D010455:Peptides; D010675:Pheromones; D012333:RNA, Messenger; D000072235:Mating Factor; D000637:Transaminases; D005945:Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing)