Efficacy of the urinary bladder for isolation of Borrelia burgdorferi from naturally infected, wild Peromyscus leucopus.
The efficacy of culturing urinary bladder tissue for Borrelia burgdorferi from naturally infected, wild Peromyscus leucopus mice was determined. The urinary bladder cultures were as efficient as spleen, kidney, and blood tissue cultures. The rapid B. burgdorferi isolation (mean, 6 days) from mouse urinary bladders should aid in defining new Lyme disease foci.
Callister
S M
SM
Microbiology Research Laboratory, Gundersen Medical Foundation, La Crosse, Wisconsin.
Agger
W A
WA
Schell
R F
RF
Brand
K M
KM
D000818:Animals; D000835:Animals, Wild / Q000382:microbiology; D001898:Borrelia / Q000302:isolation & purification*; D046508:Culture Techniques; D005455:Fluorescent Antibody Technique; D007668:Kidney / Q000382:microbiology; D051379:Mice; D010542:Peromyscus / Q000382:microbiology*; D013154:Spleen / Q000382:microbiology; D001743:Urinary Bladder / Q000382:microbiology*