title: Expression of the plum pox virus coat protein region in Escherichia coli.
abstract: A cDNA complementary to the 3' end of plum pox virus (PPV) RNA was sequenced. The sequence was investigated for the presumable coat protein cistron by computer-aided translation. A fragment containing the stop codon of the polyprotein gene and a putative virus-specific protease cleavage site was subcloned into an E. coli expression vector. It is shown by immunological analysis that the coat protein cistron is located within the subcloned region.
authors.affiliation: Institute for Applied Microbiology, University of Agriculture, Vienna, Austria.
authors.forename: D
authors.initials: D
authors.lastname: Mattanovich
authors.forename: G
authors.initials: G
authors.lastname: Himmler
authors.forename: M
authors.initials: M
authors.lastname: Laimer
authors.forename: E
authors.initials: E
authors.lastname: Maiss
authors.forename: F
authors.initials: F
authors.lastname: Regner
authors.forename: A
authors.initials: A
authors.lastname: Da Camara Machado
authors.forename: V
authors.initials: V
authors.lastname: Hanzer
authors.forename: R
authors.initials: R
authors.lastname: Casper
authors.forename: H W
authors.initials: HW
authors.lastname: Katinger
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015153:Blotting, Western; D002213:Capsid / Q000096:biosynthesis / Q000235:genetics*; D003001:Cloning, Molecular; D001330:Electronic Data Processing; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D005814:Genes, Viral; D008969:Molecular Sequence Data; D010942:Plant Viruses / Q000235:genetics*; D010957:Plasmids; D012367:RNA, Viral / Q000235:genetics; D015183:Restriction Mapping
chemical_list: D012367:RNA, Viral