title: Thiolutin inhibits utilization of glucose and other carbon sources in cells of Escherichia coli.
abstract: Thiolutin was found to inhibit the utilization of glucose and other growth substrates in Escherichia coli. The inhibition was detected by a sharp drop of the respiration rate after addition of the antibiotic. The actual function affected was allocated to the cytoplasmic membrane of the bacterial cells by the following evidence: --spheroplasts were affected like intact cells, --individual reactions of either the electron transport chain or the glycolytic pathway were not inhibited, --glucose consumption in the culture stopped and the cells accumulated guanosine tetraphosphate as under starvation conditions, --activation of the cell's apo-glucose dehydrogenase restored respiration via bypassing the glucose phosphotransferase system. It was concluded that the transport of certain substrates across the membrane was inhibited.
authors.affiliation: Institut für Allgemeine Botanik, Universität Hamburg, FRG.
authors.forename: R
authors.initials: R
authors.lastname: Bergmann
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D003470:Culture Media; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D005947:Glucose / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects; D011760:Pyrrolidinones / Q000494:pharmacology; D013104:Spheroplasts / Q000187:drug effects / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D011760:Pyrrolidinones; C006361:acetopyrrothine; D005947:Glucose