title: Structural analysis of phospho-D-mannan-protein complexes isolated from yeast and mold form cells of Candida albicans NIH A-207 serotype A strain.
abstract: The immunochemical properties between phospho-D-mannan-protein complexes of yeast (Y) and mycelial (M) forms of Candida albicans NIH A-207 (serotype A) strain were compared. Hydrolysis of the Y-form complex gave a mixture of beta-(1----2)-linked D-mannooligosaccharides consisting mainly of tri- and tetra-ose, whereas the M-form complex gave preponderantly D-mannose. The antiserum against Y-form cells exhibited a lower reactivity with the M-form than with the Y-form complex, whereas the antiserum to M-form cells could not distinguish significantly between both complexes. Moreover, these acid-modified complexes showed lower antibody-precipitating effect than each corresponding intact complex against antisera of Y- and M-form cells. Digestion of the acid-modified Y- and M-form complexes with the Arthrobacter GJM-1 strain alpha-D-mannosidase yielded 35- and 40-% degradation products, respectively. Acetolysis of each modified complex under mild conditions gave the same D-mannohexaose, beta-D-Manp-(1----2)-beta-D-Manp-(1----2)-alpha-D-Manp -(1----2)-alpha-D-Manp- (1----2)-alpha-D-Manp-(1----2)-D-Man. Because the complexes of Y- and M-form cells of C. albicans NIH B-792 (serotype B) strain did not give any hexaose fraction containing beta-(1----2) linkages, the presence of this hexaose can be regarded as one of the dominant characteristics of the serotype-A specificity of C. albicans spp.
authors.affiliation: Second Department of Hygienic Chemistry, Tohoku College of Pharmacy, Sendai, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Shibata
authors.forename: S
authors.initials: S
authors.lastname: Fukasawa
authors.forename: H
authors.initials: H
authors.lastname: Kobayashi
authors.forename: M
authors.initials: M
authors.lastname: Tojo
authors.forename: T
authors.initials: T
authors.lastname: Yonezu
authors.forename: A
authors.initials: A
authors.lastname: Ambo
authors.forename: Y
authors.initials: Y
authors.lastname: Ohkubo
authors.forename: S
authors.initials: S
authors.lastname: Suzuki
mesh_terms: D002176:Candida albicans / Q000032:analysis*; D006851:Hydrochloric Acid; D007120:Immunochemistry; D009682:Magnetic Resonance Spectroscopy; D008361:Mannosidases / Q000378:metabolism; D008745:Methylation; D011233:Precipitin Tests; D011506:Proteins / Q000032:analysis*; D012441:Saccharomyces cerevisiae / Q000032:analysis*; D013403:Sugar Phosphates / Q000032:analysis*; D043323:alpha-Mannosidase
chemical_list: D011506:Proteins; D013403:Sugar Phosphates; D008361:Mannosidases; D043323:alpha-Mannosidase; D006851:Hydrochloric Acid