Individual protein-binding domains of the insulin gene enhancer positively activate beta-cell-specific transcription.
A beta-cell-specific enhancer is located in the 5'-flanking DNA of the rat insulin 1 gene. Two homologous 8-base-pair sequences in the enhancer (IEB1 and IEB2) significantly stimulated transcription from a heterologous promoter (two- to fourfold) in a cell-specific fashion. When the elements were combined or duplicated, more than 50% of the activity of the intact enhancer was obtained. These two cis-acting elements appear to play a dominant role in the positive control of beta-cell-specific transcription of the insulin gene.
Karlsson
O
O
Department of Microbiology, University of Umea, Sweden.
Walker
M D
MD
Rutter
W J
WJ
Edlund
T
T
D000818:Animals; D001665:Binding Sites; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D004742:Enhancer Elements, Genetic; D007328:Insulin / Q000235:genetics*; D007515:Islets of Langerhans / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D051381:Rats; D014158:Transcription, Genetic
D007328:Insulin; D011506:Proteins; D015500:Chloramphenicol O-Acetyltransferase