Presence of immunoreactive endothelin in human plasma.
A highly specific and sensitive radioimmunoassay has been established for measurement of human endothelin (hET) in human plasma. After extraction of plasma with an octyl-silica column, this assay allowed for detection of immunoreactive (IR) hET as low as 0.2 fmol/ml. In 16 healthy subjects, the mean concentration of plasma IR-hET was 0.6 fmol/ml. Reverse-phase HPLC coupled with radioimmunoassay revealed two major IR-hET components, one corresponding to authentic hET(1-21) and another with more hydrophilicity than hET(1-21). These data indicate that ET is a circulating vasoconstrictor hormone in man.
Ando
K
K
Second Department of Internal Medicine, Tokyo Medical and Dental University, Japan.
Hirata
Y
Y
Shichiri
M
M
Emori
T
T
Marumo
F
F
D000328:Adult; D002851:Chromatography, High Pressure Liquid; D016232:Endothelins; D004730:Endothelium, Vascular; D005260:Female; D006801:Humans; D008297:Male; D010455:Peptides / Q000097:blood*; D011863:Radioimmunoassay; D012016:Reference Values
D016232:Endothelins; D010455:Peptides