Further characterization of the biological properties of human hematopoietic survival and growth factor.
Human hematopoietic survival and stem cell growth factor (SCGF), derived from the KPB-M15 myeloid cells, is a heat- and pH-stable protein. Chemical modification with various denaturing agents and proteolytic enzymes abrogated SCGF activity. The granulocyte-macrophage colony-potentiating and erythroid burst-promoting activities of SCGF were proportional to the density of the bone marrow (BM) cells cultured, the optimal BM cell density for delta granulocyte- and delta burst-promoting activities being 5 to 10 X 10(5)/ml. These data could be important in enabling the use of SCGF to induce proliferation of human hematopoietic stem cells in vitro.
Hiraoka
A
A
Department of Internal Medicine, Osaka Dental University, Japan.
Ohkubo
T
T
Fukuda
M
M
D001854:Bone Marrow Cells; D002452:Cell Count; D002470:Cell Survival; D002478:Cells, Cultured; D004912:Erythrocytes / Q000166:cytology; D006098:Granulocytes / Q000166:cytology; D006133:Growth Substances / Q000032:analysis / Q000494:pharmacology*; D016298:Hematopoietic Cell Growth Factors; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000502:physiology; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008264:Macrophages / Q000166:cytology; D009439:Neuraminidase; D010447:Peptide Hydrolases
D006133:Growth Substances; D016298:Hematopoietic Cell Growth Factors; D009439:Neuraminidase; D010447:Peptide Hydrolases