In situ pyrimidine dimer determination by laser cytometry.
By using antiserum against pyrimidine dimers and argon-laser imaging microspectrofluorometry, we established a new method to determine UV-induced pyrimidine dimers and their repair in individual human cells. The method was sensitive enough to determine dimers induced by UV dose as low as 2 J/m2. Normal human cells repaired 50 and 60% of total damage within 8 and 24 h after UV irradiation (20 J/m2), but Xeroderma pigmentosum cells (complementation group A) were unable to repair any within the same period. Therefore, the method proved to be a quick, easy, sensitive and accurate means to determine pyrimidine dimers in situ.
Mori
T
T
Wani
A A
AA
D'Ambrosio
S M
SM
Chang
C C
CC
Trosko
J E
JE
D004260:DNA Repair; D005455:Fluorescent Antibody Technique; D006801:Humans; D007834:Lasers; D011740:Pyrimidine Dimers / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D014466:Ultraviolet Rays
D011740:Pyrimidine Dimers