Monoclonal antibodies in detection of choroidal melanoma.
Three monoclonal antibodies (MoAbs) prepared against cutaneous melanomas were tested against one group of 12 choroidal melanomas with indirect immunofluorescence in frozen sections. A fourth MoAb was tested in paraffin sections of a second group of 47 choroidal melanomas. One MoAb (NKI-M7) did not react with choroidal melanoma, even though it had a high sensitivity for cutaneous melanoma. A second MoAb (NKI-M6) showed a positive reaction with only 2/12 choroidal melanomas. The third MoAb (NKI/beteb) reacted with all choroidal melanomas, regardless of the cell type. MoAb NKI/C-3 was positive with 38/47 (81%) choroidal melanomas. We conclude that NKI/C-3 and NKI/beteb have a high sensitivity for both cutaneous and choroidal melanomas in frozen sections. Of these two antibodies NKI/beteb was the most specific for cutaneous naevi and melanomas.
Ringens
P J
PJ
Department of Ophthalmology, Erasmus University Rotterdam, Eye Hospital, The Netherlands.
van Haperen
R
R
Vennegoor
C
C
de Jong
P T
PT
van Duinen
S G
SG
Ruiter
D J
DJ
van der Kamp
A W
AW
D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001706:Biopsy; D002830:Choroid Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D008545:Melanoma / Q000175:diagnosis* / Q000378:metabolism; D012878:Skin Neoplasms / Q000276:immunology
D000911:Antibodies, Monoclonal