title: Plasmid-mediated chloramphenicol resistance in Staphylococcus hyicus.
abstract: A small plasmid of 3.95 kb, encoding resistance to chloramphenicol (Cm) was detected in three of 33 Staphylococcus hyicus strains. The plasmid in each of the three strains was indistinguishable by Southern-blot hybridization and restriction enzyme analysis. It was shown by curing and by transformation to specify resistance to Cm. A preliminary restriction map of the plasmid, designated pSC2, is presented. Chloramphenicol acetyltransferase was demonstrated by enzyme assay and by SDS-PAGE of cell-free lysates of pSC2 transformants.
authors.affiliation: Institut für Bakteriologie, Immunologie der Justus-Liebig Universität Giessen, FRG.
authors.forename: S
authors.initials: S
authors.lastname: Schwarz
authors.forename: M
authors.initials: M
authors.lastname: Cardoso
authors.forename: H
authors.initials: H
authors.lastname: Blobel
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D015500:Chloramphenicol O-Acetyltransferase / Q000032:analysis; D002702:Chloramphenicol Resistance / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D010957:Plasmids / Q000235:genetics*; D015183:Restriction Mapping; D013210:Staphylococcus / Q000187:drug effects* / Q000235:genetics; D013552:Swine
chemical_list: D015500:Chloramphenicol O-Acetyltransferase