Association of casein kinase II with microtubules.
A magnesium-dependent heparin-inhibited protein kinase activity associated with brain microtubule preparations has been identified as casein kinase II using a monospecific polyclonal antibody. This enzyme appears enriched in cold-stable microtubule fractions. By immunofluorescence microscopy using an antiserum against casein kinase II, the in situ immunolabeling of some microtubule assays has been observed. Thus, mitotic spindles are stained by the anti-casein kinase II antibody in fibroblast cells. In neuroblastoma cells induced to differentiate, the labeling of microtubule arrays inside developing axon-like processes is also seen. These results support the view that casein kinase II can modulate cytoskeletal assembly and dynamics through phosphorylation of microtubule proteins.
Serrano
L
L
Centro de Biología Molecular (CSIC-UAM), Universidad Autónoma, Madrid, Spain.
Hernández
M A
MA
Díaz-Nido
J
J
Avila
J
J
D000818:Animals; D001369:Axons / Q000201:enzymology / Q000648:ultrastructure; D001921:Brain / Q000201:enzymology; D047388:Casein Kinases; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D008099:Liver / Q000201:enzymology; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D008870:Microtubules / Q000201:enzymology*; D009447:Neuroblastoma; D009474:Neurons / Q000201:enzymology* / Q000648:ultrastructure; D011494:Protein Kinases / Q000378:metabolism*; D008941:Spindle Apparatus / Q000201:enzymology; D014404:Tubulin / Q000378:metabolism; D014407:Tumor Cells, Cultured
D008869:Microtubule-Associated Proteins; D014404:Tubulin; D011494:Protein Kinases; D047388:Casein Kinases