Identification and partial purification of a novel tumor-derived protein that induces tissue factor on cultured human endothelial cells.
Conditioned medium of a human bladder carcinoma cell line (J82) was found to induce tissue factor synthesis in cultured human umbilical vein endothelial cells (HUVEC). A protein present in the J82 conditioned medium was partially purified by FPLC using a combination of MONO Q and Superose 6 columns. The bladder carcinoma-derived cytokine (BCDC) exhibited a Mr of 22 kDa by gel permeation HPLC. Polyclonal antibody against either interleukin-1, tumor necrosis factor, or transforming growth factor-beta failed to inhibit the ability of the conditioned medium to induce HUVEC tissue factor activity, suggesting that this tumor cell line secretes a novel cytokine responsible for HUVEC tissue factor induction.
Noguchi
M
M
Department of Pathology, University of New Mexico, School of Medicine, Albuquerque 87131.
Sakai
T
T
Kisiel
W
W
D001685:Biological Factors / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D016207:Cytokines; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D013925:Thromboplastin / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured; D014471:Umbilical Veins; D001749:Urinary Bladder Neoplasms / Q000378:metabolism*
D001685:Biological Factors; D016207:Cytokines; D009363:Neoplasm Proteins; D013925:Thromboplastin