Studies of in vivo phosphorylated proteins in BC3H-1 myocytes suggest that protein kinase C is involved in insulin action.
Insulin (10 and 100 nM) and phorbol esters increased the phosphorylation of several proteins, including 40, 47 and 80 kDa proteins, which are markers for protein kinase C activation. Insulin effects were evident at 2 min and increased over 20 min. These findings suggest that insulin activates protein kinase C in BC3H-1 myocytes.
Vila
M C
MC
Veterans Administration Hospital, Tampa, FL.
Cooper
D R
DR
Davis
J S
JS
Standaert
M L
ML
Farese
R V
RV
D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation / Q000187:drug effects; D007328:Insulin / Q000494:pharmacology*; D007525:Isoelectric Focusing; D007700:Kinetics; D008970:Molecular Weight; D009132:Muscles / Q000187:drug effects / Q000201:enzymology*; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D007328:Insulin; D010750:Phosphoproteins; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate