Production scale purification of biosynthetic human insulin by reversed-phase high-performance liquid chromatography.
A process based on reversed-phase high-performance liquid chromatography (RP-HPLC) has been developed for the purification of biosynthetic human insulin (BHI). The RP-HPLC procedure has been successfully integrated into the multimodal chromatographic production process used to purify kilogram quantities of BHI. Axial compression column technology was used in the scale-up process. The RP-HPLC procedure yields an insulin product having high chemical purity and full biological activity.
Kroeff
E P
EP
Department of Biosynthetic Isolation and Purification Development, Lilly Research Laboratories, Indianapolis, IN 46285.
Owens
R A
RA
Campbell
E L
EL
Johnson
R D
RD
Marks
H I
HI
D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000032:analysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification*; D010455:Peptides / Q000032:analysis; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification; D013056:Spectrophotometry, Ultraviolet
D007328:Insulin; D010455:Peptides; D011994:Recombinant Proteins