The effect of clomiphene on the synthesis of prostaglandins (PGF2 alpha, PGE2, PGI2) in human endometrial cells in vitro with and without addition of estradiol-17 beta or progesterone.
The production of prostaglandin F2 alpha in monolayer stromal cell cultures of proliferative human endometrium is enhanced by 10(-7) mol/l estradiol-17 beta or 10(-4) mol/l progesterone. Progesterone in high concentration (10(-4) mol/l) also enhanced the synthesis of prostaglandin E2. Clomiphene citrate reduced this increased prostaglandin production dose dependently. The synthesis of prostaglandin I2 was not influenced either by sex steroids or by clomiphene citrate.
Neulen
J
J
Department of Obstetrics and Gynecology, University of Freiburg, FRG.
Zahradnik
H P
HP
Flecken
U
U
Breckwoldt
M
M
D002478:Cells, Cultured; D002996:Clomiphene / Q000494:pharmacology*; D015237:Dinoprost / Q000096:biosynthesis*; D015232:Dinoprostone / Q000096:biosynthesis*; D004717:Endometrium / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D011464:Epoprostenol / Q000096:biosynthesis*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006801:Humans; D011374:Progesterone / Q000494:pharmacology*
D002996:Clomiphene; D011374:Progesterone; D004958:Estradiol; D015237:Dinoprost; D011464:Epoprostenol; D015232:Dinoprostone