Homocitrate is a component of the iron-molybdenum cofactor of nitrogenase.
When apodinitrogenase (lacking FeMo-co) was activated with FeMo-co synthesized in vitro in the presence of 3H-labeled homocitrate, label was incorporated into dinitrogenase. The physical association of the label with FeMo-co was demonstrated by reisolation and purification of the cofactor from dinitrogenase. The presence of homocitrate in FeMo-co was established by NMR analysis of the organic acid extracted from dinitrogenase. Quantitation of homocitrate in dinitrogenase showed it to be present at a 1:1 ratio with molybdenum.
Hoover
T R
TR
Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison 53706.
Imperial
J
J
Ludden
P W
PW
Shah
V K
VK
D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005288:Ferredoxins / Q000096:biosynthesis*; D007700:Kinetics; D007711:Klebsiella pneumoniae / Q000201:enzymology*; D009682:Magnetic Resonance Spectroscopy; D008983:Molybdoferredoxin / Q000096:biosynthesis* / Q000302:isolation & purification; D009591:Nitrogenase / Q000096:biosynthesis* / Q000302:isolation & purification; D014233:Tricarboxylic Acids / Q000032:analysis / Q000378:metabolism*
D005288:Ferredoxins; D008983:Molybdoferredoxin; D014233:Tricarboxylic Acids; C028143:homocitric acid; D009591:Nitrogenase