Thermodynamic and kinetic control of ATP synthesis in yeast mitochondria: role of delta pH.
ATP synthesis rate, measured as the variation in external Pi concentration, varied as a linear function of either delta  mu H+ or delta Gpin, in such a manner that the delta Gpin/delta  mu H+ ratio increased while VATP increased. We also observed a linear dependence of the flux control coefficient of the Pi carrier on delta pH. All the results presented can be explained by a relatively large delta pH drop when VATP increases.
Beauvoit
B
B
Institut de Biologie Cellulaire et de Neurochimie du CNRS, Bordeaux, France.
Rigoulet
M
M
Guerin
B
B
D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008928:Mitochondria / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013816:Thermodynamics
D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate