Production of interferon-beta upon induction with polyinosinic acid:polycytidylic acid during the cell cycle of human fibroblasts.
The production of interferon-beta was examined at various stages of the cell cycle in synchronized and unsynchronized cell populations induced by poly(I):poly(C). Human fibroblasts were synchronized with mitotic detachment and, at different stages of the cell cycle, poly(I):poly(C) was added for induction of interferon-beta. One hour after induction, cell-free medium was collected and assayed for secreted interferon-beta. The cells were then fixed and stained with a DNA-specific fluorochrome, 4',6-diamidino-2-phenylindole (DAPI), for cell cycle analysis by microfluorometry. The data indicated that interferon-beta was produced in every stage examined of the cell cycle. In addition, the level of intracellular interferon-beta was quantitatively measured in single cells of an unsynchronized cell population using a specific antibody. In the same individual cell, DAPI fluorescence intensity was measured for determination of the cell cycle position. The results show that interferon-beta protein can be detected throughout the cell cycle.
Tang
K F
KF
Division of Biophysics, School of Hygiene and Public Health, Johns Hopkins University, Baltimore, Maryland 21205.
Ts'o
P O
PO
Lesko
S A
SA
D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D004247:DNA / Q000032:analysis; D005347:Fibroblasts; D005455:Fluorescent Antibody Technique; D006801:Humans; D007370:Interferon Type I / Q000032:analysis / Q000096:biosynthesis*; D007399:Interphase; D007700:Kinetics; D008940:Mitotic Index; D011070:Poly I-C / Q000494:pharmacology*
D007370:Interferon Type I; D004247:DNA; D011070:Poly I-C