Effect of complement binding on a solid-phase immunometric TSH assay.
The binding of serum thyrotropin (TSH) to plastic beads coated with a monoclonal antibody to human TSH was inhibited unless EDTA was present during the incubation. The inhibitory factor in serum was heat labile, and its effect could be abrogated by the addition of human albumin-anti-albumin immune complexes. Subsequently it was shown that the antibody-coated beads were able to bind the first component of complement, C1q, and that this binding was inhibited by addition of albumin-anti-albumin complexes. The results show that a surface coated with a monoclonal murine antibody is able to bind complement, and that binding of complement may interfere in solid-phase immunometric assays.
Käpyaho
K
K
Minerva Institute for Medical Research, Kauniainen, Finland.
Tanner
P
P
Weber
T
T
D000911:Antibodies, Monoclonal / Q000378:metabolism; D000936:Antigen-Antibody Complex; D002352:Carrier Proteins; D003171:Complement Pathway, Classical; D006801:Humans; D018960:Hyaluronan Receptors; D007158:Immunologic Techniques; D008562:Membrane Glycoproteins; D024101:Mitochondrial Proteins; D011137:Polystyrenes; D011951:Receptors, Complement / Q000378:metabolism*; D011989:Receptors, Thyrotropin / Q000378:metabolism*; D012709:Serum Albumin / Q000276:immunology; D013972:Thyrotropin / Q000097:blood*
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; C087299:C1QBP protein, human; D002352:Carrier Proteins; D018960:Hyaluronan Receptors; D008562:Membrane Glycoproteins; D024101:Mitochondrial Proteins; D011137:Polystyrenes; D011951:Receptors, Complement; D011989:Receptors, Thyrotropin; D012709:Serum Albumin; C032402:complement 1q receptor; D013972:Thyrotropin