title: Acceleration of postmortem tenderization in ovine carcasses through infusion of calcium chloride: effect of concentration and ionic strength.
abstract: Ovine carcasses were arterially infused with a volume equal to 10% of the live weight after electrical stimulation. The infusion solutions contained .075 M, .15 M or .3 M calcium chloride. Results indicated that .3 M calcium chloride treatment was the most effective concentration of CaCl2 to reduce the shear force value measured at 24 h postmortem. To examine the contribution of ionic strength to tenderization that occurs by infusion of carcasses with .3 M CaCl2, ovine carcasses were infused with CaCl2 and NaCl solutions of identical ionic strength. Results indicated that the tenderization that occurred by infusion of carcasses with CaCl2 was not due to ionic strength of the CaCl2 solution. Results also indicated that, compared to control animals, NaCl-infused carcasses were more tender after 6 d of postmortem storage (but not after 1 d, as observed with CaCl2-infused carcasses). Evidence is presented that indicates that activation of calcium-dependent proteases could be responsible for the observed tenderization (reduction in shear force) due to infusion of ovine carcasses with CaCl2.
authors.affiliation: U.S. Department of Agriculture, Clay Center, NE 68933.
authors.forename: M
authors.initials: M
authors.lastname: Koohmaraie
authors.forename: J D
authors.initials: JD
authors.lastname: Crouse
authors.forename: H J
authors.initials: HJ
authors.lastname: Mersmann
mesh_terms: D000818:Animals; D002122:Calcium Chloride / Q000032:analysis / Q000494:pharmacology*; D004558:Electric Stimulation; D006863:Hydrogen-Ion Concentration; D007261:Infusions, Intra-Arterial; D009124:Muscle Proteins / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism*; D009994:Osmolar Concentration; D010447:Peptide Hydrolases / Q000378:metabolism*; D012756:Sheep; D012965:Sodium Chloride / Q000032:analysis / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors
chemical_list: D009124:Muscle Proteins; D012965:Sodium Chloride; D010447:Peptide Hydrolases; D002122:Calcium Chloride