[Studies on regulation of the function of thermogenic tissue, brown adipose tissue (BAT) by means of tissue culture method].
In order to clarify the regulatory mechanism of BAT function, the effects of noradrenaline (NA) (6mM), insulin (I) (40nM), glucagon (G) (400nM) and nerve growth factor (NGF) (2-10nM), alone or in combination, were investigated directly in BAT from neonatal rats (ca. 3 days old), cultured in 10% fetal bovine serum-medium 199 in 95% air-5% CO2 gas phase at 33 degrees C for 1 to 2 weeks. I stimulated lipid accumulation and enlarged outgrown cell size, but mitochondria in the cells of tissue block were smaller and their cristae less distinct. I + G enlarged nucleus and cytoplasm, and suppressed the lipid accumulation induced by I, but mitochondria in the cells of tissue block were larger and their cristae became more prominent than those of I-added cells. G induced the similar changes to those by I + G. I + NA also induced the similar effects to those by I + G, but their mitochondria size did not differ from that of I-added cells. NGF caused the similar effects of those by G, inducing the development of mitochondria, rough endoplasmic reticulum and Golgi complex. These results suggest that multiple factors such as NA, I, G and NGF regulate differentiation and functional development of BAT.
Yahata
T
T
Department of Physiology, Asahikawa Medical College, Japan.
Kuroshima
A
A
D000273:Adipose Tissue / Q000502:physiology* / Q000648:ultrastructure; D000818:Animals; D001833:Body Temperature Regulation; D002478:Cells, Cultured; D005934:Glucagon / Q000502:physiology; D007328:Insulin / Q000502:physiology; D008854:Microscopy, Electron; D009414:Nerve Growth Factors / Q000502:physiology; D009638:Norepinephrine / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains
D007328:Insulin; D009414:Nerve Growth Factors; D005934:Glucagon; D009638:Norepinephrine