Patch clamp recording from anterior pituitary cells identified by reverse hemolytic plaque assay.
The study of hormone secretion by anterior pituitary cells is complicated by the presence of multiple cell types. For unambiguous interpretation of data it is necessary to identify the cells from which measurements are made. We have described a reliable experimental approach involving the identification of cultured cells of a particular type with a reverse hemolytic plaque assay. The electrical characteristics of individual identified cells can then be studied using patch clamp recording. This electrophysiological approach is well suited to the study of complex systems in cultured cells. Although this combined approach requires some expertise in a variety of techniques, it is workable and should yield valuable information regarding the role of ion channels in the cellular control of hormone secretion by the anterior pituitary.
Croxton
T L
TL
Armstrong
W M
WM
Ben-Jonathan
N
N
D000818:Animals; D002462:Cell Membrane / Q000502:physiology; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods; D004594:Electrophysiology / Q000295:instrumentation / Q000379:methods; D006462:Hemolytic Plaque Technique; D007106:Immune Sera; D007202:Indicators and Reagents; D010903:Pituitary Gland, Anterior / Q000378:metabolism / Q000502:physiology*; D010908:Pituitary Hormones, Anterior / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D007106:Immune Sera; D007202:Indicators and Reagents; D010908:Pituitary Hormones, Anterior