Topological analysis of the amino-terminal region of lactose permease using the Escherichia coli outer membrane protein, OmpA, as a marker.
LacY-ompA fusions, encoding the N-terminal 50, 71 or 143 residues of lactose permease, were constructed. The observed orientation of the OmpA part of each hybrid protein with respect to the plasma membrane supports current models of the N-terminus of Lac permease. Hybrids possessing the entire mature OmpA were very stable; those with only a part thereof were much less stable. Due to their in vivo stability and accessibility to antibody it is proposed that such hybrids may represent potential models to investigate the assembly pathway of lactose permease.
MacIntyre
S
S
Max-Planck-Institut für Biologie, Tübingen, FRG.
Eschbach
M L
ML
Schwarz
H
H
Ehring
R
R
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology; D003001:Cloning, Molecular; D003062:Codon; D003600:Cytosol / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins; D006358:Hot Temperature; D007150:Immunohistochemistry; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D008854:Microscopy, Electron; D009004:Monosaccharide Transport Proteins; D010446:Peptide Fragments / Q000378:metabolism; D010957:Plasmids; D027981:Symporters; D014357:Trypsin / Q000378:metabolism
D001425:Bacterial Outer Membrane Proteins; D003062:Codon; D029968:Escherichia coli Proteins; C050221:LacY protein, E coli; D026901:Membrane Transport Proteins; D009004:Monosaccharide Transport Proteins; D010446:Peptide Fragments; D027981:Symporters; C021499:lactose permease; D014357:Trypsin