Cellular interaction in cartilage degradation.
Rat femoral head cartilages (FHCs) in culture for six days retained their glycosaminoglycan (GAG) content and were not affected by recombinant human interleukin-la (IL-1), conditioned media from concanavalin-A stimulation of sensitized lymphocytes (lymphocyte media), or conditioned media from lymphokine-stimulated macrophages (macrophage media). Similarly FHCs cultured with macrophages in the presence of IL-1, lymphocyte medium or macrophage medium did not suffer a loss of GAG. However, FHCs cultured with fibroblasts showed a substantial loss of GAG. This fibroblast-mediated cartilage degradation was significantly increased by IL-1 and macrophage media respectively. Stimulation of fibroblasts by lymphocyte media failed to increase the loss of GAG. It is proposed that cartilage degradation in inflammatory joint disease is brought about by a sequential cell-to-cell interaction via cytokines leading ultimately to stimulation of fibroblast-mediated cartilage degradation.
Desa
F M
FM
Department of Experimental Pathology, St. Bartholomew's Hospital Medical College, London, England.
Moore
A R
AR
Chander
C L
CL
Colville-Nash
P
P
Howat
D W
DW
Willoughby
D A
DA
D000818:Animals; D002356:Cartilage / Q000378:metabolism / Q000473:pathology* / Q000502:physiology; D002357:Cartilage Diseases / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D002450:Cell Communication / Q000502:physiology; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003470:Culture Media / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D006025:Glycosaminoglycans / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects / Q000502:physiology; D008222:Lymphokines / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D011919:Rats, Inbred Strains
D003470:Culture Media; D006025:Glycosaminoglycans; D008222:Lymphokines; D003208:Concanavalin A