Effects of verapamil and diltiazem on hyperthermic cell death in CHO cells.
Non-toxic concentrations of verapamil (0.05 and 0.075 mM) and diltiazem (0.10 and 0.25 mM) sensitize CHO cells to heat killing at 44 degrees C. These drugs sensitize by reducing both the shoulder and the slope of the exponential survival curve. Exposure to verapamil or diltiazem in conjunction with the heat sensitizer procaine HCl at 44 degrees C reduced cell survival below that observed for procaine plus heat. The mechanism by which verapamil and diltiazem sensitize CHO cells to heat killing is not understood.
Coss
R A
RA
Department of Radiation Oncology and Nuclear Medicine, Thomas Jefferson University Hospital, Philadelphia, PA 19107.
Felgar
R E
RE
Stokes
D G
DG
Smith
N N
NN
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D006224:Cricetinae; D003412:Cricetulus; D004110:Diltiazem / Q000494:pharmacology*; D005260:Female; D006358:Hot Temperature; D010053:Ovary; D014700:Verapamil / Q000494:pharmacology*
D014700:Verapamil; D004110:Diltiazem