A monoclonal antibody (BL2-10D1) reacting with a bladder-cancer-associated antigen.
A hybridoma cell line secreting an IgM monoclonal antibody (MAb) was produced after immunizing a mouse with RT4 cells and a crude suspension of human bladder carcinoma cells (WHO grades II and III TCC). This MAb reacted with RT4 target cells derived from a human transitional bladder cancer but failed to react with a majority of non-bladder cancer cell lines. Immunohistological studies indicate that this MAb reacts inconstantly with normal bladder: in positive cases only a few superficial cells (5% to 10% umbrella cells) are stained but not intermediate or basal cells of the urothelium. This MAb was evaluated on 118 tumors: it reacted with tumor tissue in a majority of grade I (79.5%) and grade II papillary TCC (77.3%), less with grade III papillary TCC (45%) and very rarely with invasive non-papillary TCC (14%). In cases of flat lesions a strong reactivity of superficial, intermediate and/or basal layer cells was observed in 50% of moderate and severe dysplasia and in all cell layers of carcinomas in situ (CIS)(9/9).
Longin
A
A
Centre Léon Bérard, Lyon, France.
Hijazi
A
A
Berger-Dutrieux
N
N
Escourrou
G
G
Bouvier
R
R
Richer
G
G
Mironneau
I
I
Fontanière
B
B
Devonec
M
M
Laurent
J C
JC
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000912:Antibodies, Neoplasm / Q000276:immunology*; D002278:Carcinoma in Situ / Q000276:immunology*; D002295:Carcinoma, Transitional Cell / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D014407:Tumor Cells, Cultured / Q000276:immunology; D001743:Urinary Bladder / Q000276:immunology; D001749:Urinary Bladder Neoplasms / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm