Pyruvate decarboxylase is like acetolactate synthase (ILV2) and not like the pyruvate dehydrogenase E1 subunit.
Protein sequences of pyruvate decarboxylase (PDC) derived from cloned yeast (Saccharomyces cerevisiae) and bacterial (Zymomonas mobilis) genes were compared with each other and with sequence databases. Extensive sequence similarities were found between them and with two others: cytochrome-linked pyruvate oxidase from Escherichia coli and acetolactate synthase (ilvI in E. coli; ILV2 gene in S. cerevisiae). All catalyse decarboxylation of pyruvate using thiamine pyrophosphate (TPP) as cofactor. General overall similarity suggests common ancestry for these enzymes. None of the sequences was similar to the E1 component of pyruvate dehydrogenase from E. coli which also decarboxylates pyruvate with the help of TPP.
Green
J B
JB
European Molecular Biology Laboratory, Heidelberg, FRG.
D000095:Acetolactate Synthase / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D005075:Biological Evolution; D002262:Carboxy-Lyases / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology; D006090:Gram-Negative Bacteria / Q000201:enzymology*; D008969:Molecular Sequence Data; D007652:Oxo-Acid-Lyases / Q000235:genetics*; D011767:Pyruvate Decarboxylase / Q000235:genetics*; D011768:Pyruvate Dehydrogenase Complex / Q000235:genetics*; D011771:Pyruvate Oxidase / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D012689:Sequence Homology, Nucleic Acid; D012984:Software
D011768:Pyruvate Dehydrogenase Complex; D011771:Pyruvate Oxidase; D000095:Acetolactate Synthase; D002262:Carboxy-Lyases; D011767:Pyruvate Decarboxylase; D007652:Oxo-Acid-Lyases