Balancing of mitochondrial and glycolytic ATP production and of the ATP-consuming processes of Ehrlich mouse ascites tumour cells in a high phosphate medium.
A balance of energy budgeting of Ehrlich mouse ascites tumour cells including mitochondrial and glycolytic ATP production and about 80% of ATP consumption in a high phosphate medium is presented. In the share of glycolysis was about one-third of the total ATP production, more than twice that found in a low phosphate medium. The extent of a single energy reaction was assessed from the decrease of coupled oxygen consumption and lactate formation following the specific inhibition of this process. The inhibitory effects on coupled respiration and glycolysis were identical for the energy consuming processes measured: protein turnover, Na+/K(+)-ATPase, Ca2(+)-transport and RNA synthesis.
Schmidt
H
H
Department of Pediatrics, Humboldt University (Charite), Berlin, GDR.
Siems
W
W
Müller
M
M
Dumdey
R
R
Jakstadt
M
M
Rapoport
S M
SM
D000255:Adenosine Triphosphate / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D003470:Culture Media; D005260:Female; D006019:Glycolysis; D007773:Lactates / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D051379:Mice; D008813:Mice, Inbred ICR; D008928:Mitochondria / Q000378:metabolism*; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption; D010710:Phosphates
D003470:Culture Media; D007773:Lactates; D010710:Phosphates; D000255:Adenosine Triphosphate