[Aspergillus fumigatus: evaluations of the standardization of diagnostic extracts].
Extracts of Aspergillus fumigatus used for serodiagnosis of allergic diseases are of unknown and nonstandardized composition. Applying immunoprint technique subsequent to isoelectric focusing commercial and self-prepared extracts are compared. By use of an ABPA-pool serum and monoclonal antibodies recognizing antigens of this fungus, different immunological reactivities of the extracts can be demonstrated. It is suggested to use monoclonal antibodies as secondary standards as well as to improve standardization.
Trompelt
J S
JS
Medizinische Klinik des Forschungsinstituts Borstel.
Becker
W M
WM
Schlaak
M
M
D000911:Antibodies, Monoclonal / Q000592:standards; D000946:Antigens, Fungal / Q000276:immunology* / Q000592:standards; D001232:Aspergillus fumigatus / Q000276:immunology*; D005656:Fungal Proteins / Q000276:immunology* / Q000592:standards; D007525:Isoelectric Focusing
D000911:Antibodies, Monoclonal; D000946:Antigens, Fungal; D005656:Fungal Proteins
Aspergillus fumigatus: Ansätze zur Standardisierung von Extrakten für die Diagnostik.