Characterization of HTLV-I in a T-cell line established from a patient with myelopathy.
We have established an interleukin 2-dependent, OKT4-positive T-cell line, named HCT-1, from cells in the cerebrospinal fluid obtained from a patient with human T-cell lymphotropic virus type I (HTLV-1)-associated myelopathy. Antigens for HTLV-I were detected in HCT-1 cells by indirect immunofluorescence and Western blot testing, and type C virus particles were detected by electron microscopy. Southern blot analysis of HCT-1 cellular DNA, using an HTLV-I probe, revealed that the integrated provirus genome could not be distinguished from the HTLV-I genome in adult patients with T-cell leukemia.
Nakamura
T
T
First Department of Internal Medicine, Nagasaki, University School of Medicine, Japan.
Tsujihata
M
M
Shirabe
S
S
Matsuo
H
H
Ueki
Y
Y
Nagataki
S
S
D015139:Blotting, Southern; D015153:Blotting, Western; D002460:Cell Line; D015342:DNA Probes; D005455:Fluorescent Antibody Technique; D015486:HTLV-I Antigens / Q000032:analysis; D015490:HTLV-I Infections / Q000134:cerebrospinal fluid / Q000382:microbiology*; D015368:Human T-lymphotropic virus 1 / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D013118:Spinal Cord Diseases / Q000134:cerebrospinal fluid / Q000382:microbiology*; D013601:T-Lymphocytes / Q000382:microbiology*; D014771:Virion / Q000302:isolation & purification
D015342:DNA Probes; D015486:HTLV-I Antigens