Human fetal liver as a valuable source of haemopoietic stem cells for allogeneic bone marrow transplantation.
The CFU-GM and T cell contents of human fetal livers were studied at various times between 6-14 weeks of gestation. The number of CFU-GM increased parallel to gestational age, especially after week 10. Cells bearing mature T cell markers, however, were found only in one case out of 35 fetal liver samples. Cryopreservation of fetal liver cells hardly affected the viability and proliferative capacity of CFU-GM in the sample. According to these findings fetal liver is, at least up to the 14th gestational week, practically free of mature T cells but it does contain a considerable amount of CFU-GM (an accepted indicator of pluripotent stem cell content), consequently fetal liver can be considered as a valuable source of haemopoietic stem cells for allogeneic bone marrow transplantation for children.
Phan
D T
DT
National Institute of Haematology and Blood Transfusion, Budapest, Hungary.
Mihalik
R
R
Benczur
M
M
Pálóczi
K
K
Gidáli
J
J
Fehér
I
I
Dömötöri
J
J
Kiss
C
C
Petrányi
G G
GG
Natonek
K
K
D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D016026:Bone Marrow Transplantation; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D005260:Female; D005615:Freezing; D006098:Granulocytes / Q000166:cytology; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000196:embryology*; D006801:Humans; D008099:Liver / Q000196:embryology; D008264:Macrophages / Q000166:cytology; D011247:Pregnancy; D011309:Preservation, Biological; D013601:T-Lymphocytes / Q000166:cytology / Q000196:embryology*; D014184:Transplantation, Homologous
D000945:Antigens, Differentiation, T-Lymphocyte