Variation of movement characteristics with washing and capacitation of spermatozoa. I. Univariate statistical analysis and detection of sperm hyperactivation.
Movement alterations as spermatozoa are washed and capacitated in preparation for the zona-free sperm penetration assay were investigated using a videomicrographic computer-assisted system for tracking and analysis of sperm trajectories. Thirty-three semen samples from infertile men and 17 fertile controls were studied. Despite significant differences on the sperm penetration assay, univariate comparisons of movement characteristics between infertile and control samples failed to detect differences. For all samples combined, significant alterations in movement characteristics were detected after washing. After an 18-hour sperm capacitation, mean velocity, head yawing amplitude, and head yawing frequency still were significantly increased over seminal plasma values. Videomicrographic motion analysis thus allows detection of changes that are consistent with hyperactivated motility, as has been described for capacitating spermatozoa.
Ginsburg
K A
KA
Department of Obstetrics and Gynecology, Wayne State University, Detroit, Michigan.
Sacco
A G
AG
Moghissi
K S
KS
Sorovetz
S
S
D003936:Diagnosis, Computer-Assisted; D006801:Humans; D008297:Male; D013075:Sperm Capacitation; D013077:Sperm Head / Q000502:physiology; D013081:Sperm Motility; D013223:Statistics as Topic