title: Identification of specific binding sites for vasoactive intestinal peptide in rat testis Leydig cells and study of developmental changes.
abstract: The interaction of vasoactive intestinal peptide (VIP) with isolated Leydig cells from rat testis was time- and temperature-dependent, as well as saturable and specific. Scatchard analysis suggested the presence of both high- and low-affinity binding sites with KD values of 1.7 and 43 nM, respectively, and receptor concentrations of 35 and 1394 fmol VIP bound/mg protein in mature (3- to 6-month old) rats. When considering pubertal (45-day old) rats, the affinities were similar but the binding capacities showed considerably lower values (25 and 193 fmol VIP bound/mg protein) indicating that VIP receptors are subject to developmental changes during animal maturation.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá, Alcalá de Henares-Madrid, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Hueso
authors.forename: M J
authors.initials: MJ
authors.lastname: Carmena
authors.forename: J C
authors.initials: JC
authors.lastname: Prieto
mesh_terms: D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D066298:In Vitro Techniques; D007985:Leydig Cells / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013737:Testis / Q000254:growth & development*; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*
chemical_list: D014660:Vasoactive Intestinal Peptide