title: A mammalian translation initiation factor can substitute for its yeast homologue in vivo.
abstract: The translation initiation factor 4E (eIF-4E) is involved in the recognition of the cap structure at the 5' end of eukaryotic mRNA and facilitates ribosome binding. Subsequently, additional initiation factors mediate ribosomal scanning of mRNA and initiator AUG recognition (Shatkin, A. J. (1985) Cell 40, 223-224; Rhoads, R. E. (1988) Trends Biochem. Sci. 13, 52-56; Edery, I., Pelletier, J., and Sonenberg, N. (1987) in Translational Regulation of Gene Expression (Ilan, J., ed) pp. 335-366, Plenum Publishing Corp., New York). We show here that initiation factor 4E is functionally conserved between the unicellular eukaryote Saccharomyces cerevisiae and mammals. Although the amino acid identity of the factors from both species is limited to only 33%, mouse eIF-4E can substitute for yeast eIF-4E in vivo without major effects on cell viability, growth, and mating. This finding provides a starting point for new experimental strategies to investigate the structure-function relationship of eukaryotic translation initiation factor eIF-4E.
authors.affiliation: Institut für Biochemie und Molekularbiologie der Universität Bern, Switzerland.
authors.forename: M
authors.initials: M
authors.lastname: Altmann
authors.forename: P P
authors.initials: PP
authors.lastname: Müller
authors.forename: J
authors.initials: J
authors.lastname: Pelletier
authors.forename: N
authors.initials: N
authors.lastname: Sonenberg
authors.forename: H
authors.initials: H
authors.lastname: Trachsel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D039561:Eukaryotic Initiation Factor-4E; D005796:Genes; D005800:Genes, Fungal; D051379:Mice; D008969:Molecular Sequence Data; D010448:Peptide Initiation Factors / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D010957:Plasmids; D012270:Ribosomes / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D012689:Sequence Homology, Nucleic Acid
chemical_list: D039561:Eukaryotic Initiation Factor-4E; D010448:Peptide Initiation Factors