Gametocytogenesis induction in cultured Plasmodium falciparum and further development of the gametocytes to ookinetes in prolonged culture.
Gametocytogenesis induction medium (RPMI-FSC reactive medium), prepared with a mixture of the culture supernatant from anti-Plasmodium falciparum antibody-producing hybridoma cells and hybridoma cell lysate, consistently induced gametocytogenesis in cultured P. falciparum. Gametocytogenesis was induced in three strains (0662, FCB 1, and FCR-3) and one clone (R.FCR-3) as has previously been described in two other strains (Ono et al. 1986). Both caffeine (2 mM/ml) and calmodulin (5 micrograms/ml) enhanced the effects of RPMI-FSC reactive medium. Furthermore, gametocytes that appeared after treatment with RPMI-FSC reactive medium continued their development to ookinete formation in candle jar culture with Waymouth's medium.
Ono
T
T
Department of Protozoology, Osaka University, Japan.
Nakabayashi
T
T
D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D002147:Calmodulin / Q000494:pharmacology; D003470:Culture Media; D005718:Gametogenesis / Q000187:drug effects; D006825:Hybridomas; D010963:Plasmodium falciparum / Q000187:drug effects / Q000502:physiology*
D002147:Calmodulin; D003470:Culture Media; D002110:Caffeine