Simple three-dimensional imaging of HRP-labelled neurons with the aid of an image processor.
A method is described for visualizing the three-dimensional structure of horseradish peroxidase-labelled neurons in the central nervous system of Xenopus laevis tadpoles. The reconstruction is simple, and performed on a commercially available image processing system. Minimal operational skill is required for using this method. Also, various improvements were made where user's simplicity (user-friendliness), speed and required computer memory are concerned. In addition, the excellent properties of the IBAS II system, such as image filtering and image editing functions are fully available, offering a fast, clean and complete final image reconstruction.
van der Linden
J A
JA
Department of Anatomy and Embryology, University of Nijmegen, The Netherlands.
Terlou
M
M
Kramer
M
M
D000465:Algorithms; D000818:Animals; D001921:Brain / Q000166:cytology*; D003196:Computer Graphics; D003201:Computers; D006735:Horseradish Peroxidase; D007091:Image Processing, Computer-Assisted; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D009474:Neurons / Q000648:ultrastructure*; D014982:Xenopus laevis
D006735:Horseradish Peroxidase