title: [Measurement of DNA damage in forestomach squamous epithelium by alkaline elution assay].
abstract: The alkaline elution assay were applied for assessing DNA damage of forestomach squamous epithelium in the male F344 rat. The animals were starved for 18 hr and then given a single oral dose of the sample in saline or corn oil. After 3 hr the stomach was removed, opened along the greater curvature and washed with cold 0.024M EDTA-0.075M NaCl (pH 7.5). The mucosa of the forestomach was scraped off gently with the edge of a cover glass, and the stratified squamous epithelium was collected by scraping off firmly with the edge of a cover glass. Since the collected epithelium cells adhere to the pipettes, these cells were transferred onto a polycarbonate filter by using a micro spatula. The dose-dependent DNA damages (5 approximately 150 mg/kg) were observed after administration of 1-methyl-3-nitro-1-nitrosoguanidine (MNNG), a forestomach carcinogen.
authors.forename: K
authors.initials: K
authors.lastname: Morimoto
authors.forename: H
authors.initials: H
authors.lastname: Kitsutaka
authors.forename: K
authors.initials: K
authors.lastname: Tsuji
authors.forename: A
authors.initials: A
authors.lastname: Takahashi
authors.forename: F
authors.initials: F
authors.lastname: Furukawa
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
authors.forename: Y
authors.initials: Y
authors.lastname: Hayashi
mesh_terms: D000818:Animals; D015197:Carcinogenicity Tests / Q000379:methods*; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D004848:Epithelium; D005753:Gastric Mucosa / Q000166:cytology; D008297:Male; D008769:Methylnitronitrosoguanidine / Q000633:toxicity; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D008769:Methylnitronitrosoguanidine