[Activity of lysosomal proteinases in the liver, spleen, thymus and peritoneal macrophages after immunization of mice with thymus-dependent and thymus-independent antigens].
Mice of the CBAxC97B/6 strain were immunized with sheep erythrocytes at a dose of 0.5 ml containing 5% and 25% of the cell suspension and with Vi-antigen at doses of 2 micrograms/ml and 10 micrograms/ml, respectively. Sheep erythrocytes caused dose-dependent stimulation of cathepsin B, C and H in spleen, whereas cathepsin B was activated 3.1-3.6-fold after administration of Vi-antigen. Functional state of the lysosomal proteolytic system did not alter in thymus in response to sheep erythrocytes, while Vi-antigen activated distinctly thiol-dependent proteinases. In peritoneal macrophages administration of sheep erythrocytes led to 2-5-fold decrease in activity of all the lysosomal proteinases studied (cathepsins A, B, C, D, H and L), however Vi-antigen exhibited direct dose-dependent effect on activity of cathepsins A, B, D and L. The data obtained suggest that T-independent reactions of the immune system were realized via thiol-dependent lysosomal proteinases.
Vasil'ev
A V
AV
Vorobeĭchik
T A
TA
Pletsityĭ
K D
KD
Tutel'ian
V A
VA
D000818:Animals; D000955:Antigens, T-Independent / Q000276:immunology*; D010450:Endopeptidases / Q000378:metabolism*; D004912:Erythrocytes / Q000276:immunology*; D007114:Immunization; D008099:Liver / Q000201:enzymology; D008247:Lysosomes / Q000201:enzymology*; D008264:Macrophages / Q000201:enzymology; D051379:Mice; D010529:Peritoneal Cavity / Q000166:cytology; D012756:Sheep; D013154:Spleen / Q000201:enzymology; D013950:Thymus Gland / Q000201:enzymology
D000955:Antigens, T-Independent; D010450:Endopeptidases
Aktivnost' lizosomal'nykh proteinaz v pecheni, selezenke, timuse i peritoneal'nykh makrofagakh mysheĭ pri immunizatsii timuszavisimym i timusnezavisimym antigenami.