title: Evidence for the presence of DNA primase in mitochondria of Saccharomyces cerevisiae.
abstract: Chromatography of a DNA polymerase preparation from mitochondria of Saccharomyces cerevisiae on DNA-cellulose column, using Tris-HCl (pH 7.5) buffer containing 0.6 M NaCl as eluent, was found to yield a fraction exhibiting DNA primase-like activity free of DNA polymerase. This fraction could support the synthesis of 12-15 residue-long oligoribonucleotides on single-stranded natural or synthetic DNA templates. The oligoribonucleotides could be further elongated by incorporation of deoxyribonucleotides in the presence of Klenow fragment.
authors.affiliation: Biochemistry Division, Bhabha Atomic Research Centre, Bombay, India.
authors.forename: S D
authors.initials: SD
authors.lastname: Desai
authors.forename: K
authors.initials: K
authors.lastname: Pasupathy
authors.forename: K G
authors.initials: KG
authors.lastname: Chetty
authors.forename: D S
authors.initials: DS
authors.lastname: Pradhan
mesh_terms: D005591:Chemical Fractionation; D019915:DNA Primase; D004261:DNA Replication; D004271:DNA, Fungal / Q000302:isolation & purification / Q000378:metabolism* / Q000502:physiology; D004272:DNA, Mitochondrial / Q000302:isolation & purification / Q000378:metabolism* / Q000502:physiology; D004259:DNA-Directed DNA Polymerase / Q000302:isolation & purification / Q000502:physiology; D004591:Electrophoresis, Polyacrylamide Gel; D012316:RNA Nucleotidyltransferases / Q000302:isolation & purification / Q000378:metabolism* / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D012967:Sodium Dodecyl Sulfate; D013698:Templates, Genetic
chemical_list: D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D012967:Sodium Dodecyl Sulfate; D019915:DNA Primase; D012316:RNA Nucleotidyltransferases; D004259:DNA-Directed DNA Polymerase