title: Chromosomal transfer and in vivo cloning of genes in Vibrio cholerae using RP4::mini-Mu.
abstract: The RP4::mini-Mu replicon has been used to transfer chromosomal genes by conjugation and to clone in vivo metabolic, toxin and flagellar genes of Vibrio cholerae. RP4::mini-Mu was introduced into several strains of V. cholerae and these strains were mated with V. cholerae or Escherichia coli K-12 recipients. R'-episomes carrying the respective genes were maintained in recA recipients and were detected by complementation of auxotrophic, nontoxinogenic and aflagellate mutations in V. cholerae.
authors.affiliation: Division of Microbial Genetics, Central Drug Research Institute, Lucknow, India.
authors.forename: R
authors.initials: R
authors.lastname: Srivastava
authors.forename: V B
authors.initials: VB
authors.lastname: Sinha
authors.forename: B S
authors.initials: BS
authors.lastname: Srivastava
mesh_terms: D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000235:genetics; D004768:Enterotoxins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005407:Flagella / Q000648:ultrastructure; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D005822:Genetic Vectors; D009154:Mutation; D011815:R Factors; D012093:Replicon; D014734:Vibrio cholerae / Q000235:genetics* / Q000254:growth & development
chemical_list: D004269:DNA, Bacterial; D004768:Enterotoxins; C037420:stN protein, Vibrio cholerae