title: Cardiomyopathy in Cebus apella monkeys experimentally infected with Trypanosoma cruzi.
abstract: Twenty four Cebus apella monkeys were studied as a biological model for the cardiac chronic form of Chagas' disease. Twelve were inoculated with Trypanosoma cruzi trypomastigotes, seven with the Brazilian Y strain and five with the Argentinian RA strain. Twelve monkeys were uninfected controls. The following parameters were studied: body weight, body temperature, direct parasitemia, xenodiagnosis, specific antibodies by IFA, clinical chemistry, hematology, ECG and chest X-ray. Three monkeys infected with Y strain were sacrificed at 4 months and 4 monkeys at 12 months after inoculation. Monkeys inoculated with RA strain were sacrificed at 48 months. Direct parasitemia was positive within a week after inoculation in all monkeys. Xenodiagnosis was positive until 49.0 +/- 3.0 and 79.0 +/- 6.0 weeks p.i. for Y and RA strains, respectively. In all inoculated monkeys an increase in antibody titers was detected within 3 weeks after inoculation. In all monkeys inoculated with the Y strain and 3/5 with the RA strain abnormal ECGs were observed within 1 or 2 weeks p.i., becoming more severe in the chronic phase. Y strain inoculated monkeys sacrificed at 4 months presented only a slight concentric hypertrophy of the heart left ventricle. Those sacrificed at 12 months had concentric left ventricle hypertrophy and 3/4 had an aneurism of the apex. Four out of 5 RA strain inoculated monkeys had an enlarged, flaccid heart; 3/5 aneurism of the apex and 2/5 concentric hypertrophy of the left ventricle.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción, Paraguay.
authors.forename: I M
authors.initials: IM
authors.lastname: Rosner
authors.forename: J
authors.initials: J
authors.lastname: Bellasai
authors.forename: A
authors.initials: A
authors.lastname: Schinini
authors.forename: T
authors.initials: T
authors.lastname: Rovira
authors.forename: A R
authors.initials: AR
authors.lastname: de Arias
authors.forename: E A
authors.initials: EA
authors.lastname: Ferro
authors.forename: E
authors.initials: E
authors.lastname: Ferreira
authors.forename: G
authors.initials: G
authors.lastname: Velazquez
authors.forename: M I
authors.initials: MI
authors.lastname: Monzón
authors.forename: M
authors.initials: M
authors.lastname: Maldonado
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis; D002427:Cebidae / Q000469:parasitology*; D002428:Cebus / Q000469:parasitology*; D002598:Chagas Cardiomyopathy / Q000097:blood / Q000473:pathology* / Q000503:physiopathology; D004195:Disease Models, Animal; D004562:Electrocardiography; D005260:Female; D005455:Fluorescent Antibody Technique; D008297:Male; D009206:Myocardium / Q000473:pathology*; D014349:Trypanosoma cruzi / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000913:Antibodies, Protozoan