Mutations in the maize mitochondrial T-urf13 gene eliminate sensitivity to a fungal pathotoxin.
URF13, the product of the mitochondrial T-urf13 gene, confers on Texas cytoplasmic male-steril maize (Zea mays L.) a unique susceptibility to a fungal pathogen (Bipolaris maydis race T) and sensitivity to its pathotoxin. Expression of URF13 in Escherichia coli imparts pathotoxin sensitivity to the bacterium. We show by ion uptake studies in E. coli that a pathotoxin-URF13 interaction causes membrane permeability. Similarly, mitochondrial dysfunction caused by membrane permeabilization probably accounts for increased colonization of maize carrying the Texas cytoplasm by toxin-producing pathogens. Site-directed mutagenesis studies show that approximately one-quarter of the amino acids at the carboxyl end of URF13 can be eliminated without affecting toxin sensitivity. We have identified two dicyclohexylcarbodiimide (DCCD) binding sites in the URF13 protein and show that one of the sites is involved in conferring DCCD protection against the pathotoxin. Substitutional mutations at this DCCD binding site also eliminate toxin sensitivity.
Braun
C J
CJ
Department of Genetics, North Carolina State University, Raleigh 27695-7614.
Siedow
J N
JN
Williams
M E
ME
Levings
C S
CS
D000595:Amino Acid Sequence; D002462:Cell Membrane / Q000378:metabolism; D004272:DNA, Mitochondrial / Q000235:genetics*; D004024:Dicyclohexylcarbodiimide / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D005796:Genes; D007700:Kinetics; D008928:Mitochondria / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D009183:Mycotoxins / Q000494:pharmacology*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D010944:Plants / Q000187:drug effects / Q000235:genetics*; D011485:Protein Binding; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000378:metabolism; D003313:Zea mays / Q000187:drug effects / Q000235:genetics
D004272:DNA, Mitochondrial; D009183:Mycotoxins; D010940:Plant Proteins; D011994:Recombinant Proteins; D004024:Dicyclohexylcarbodiimide