Effector cell expression of NK1.1, a murine natural killer cell-specific molecule, and ability of mice to reject bone marrow allografts.
The rejection of Hh-1 incompatible bone marrow cells in irradiated mice is mediated by NK cells and is genetically regulated. We tested the role of the NK-specific gene, NK1.1, in regulating the rejection of allogeneic bone marrow cell grafts. NK1.1+ mice, that are known to display strong resistance against Hh-1 incompatible grafts, were crossed to H-2/Hh-1 identical NK1.1-, poor responder mice, and the progeny were backcrossed to the poor responder parent. The segregating mice were individually typed for their expression of NK1.1 and the ability to resist Hh-1 incompatible bone marrow cells (BMC). A strong correlation was noted between expression of NK1.1 and rejection of H-2d/Hh-1d BMC. Our results support the idea that NK1.1 is one of the genes responsible for strong resistance to Hh-1d (determinant 2) but not for Hh-1j (determinant 3) BMC grafts. We suggest that the NK1.1 molecule functions as an accessory molecule in the cellular interactions involving the recognition of Hh-1 determinants.
Sentman
C L
CL
Department of Pathology, University of Texas Southwestern Medical Center, Dallas 75235.
Kumar
V
V
Koo
G
G
Bennett
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D016026:Bone Marrow Transplantation; D003433:Crosses, Genetic; D006084:Graft Rejection; D006183:H-2 Antigens / Q000235:genetics; D006649:Histocompatibility Antigens / Q000235:genetics; D007113:Immunity, Innate; D007694:Killer Cells, Natural / Q000276:immunology* / Q000378:metabolism; D055646:Killer Factors, Yeast; D008213:Lymphocyte Activation; D051379:Mice; D008805:Mice, Inbred A; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D008814:Mice, Inbred NZB; D011506:Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D006183:H-2 Antigens; C056907:Hh1 protein, mouse; D006649:Histocompatibility Antigens; D055646:Killer Factors, Yeast; D011506:Proteins