The effect of gamma-radiation on smoked fish using short-term mutagenicity assays.
The effect of gamma-radiation on the mutagenicity potential of wood-smoked fish (Rastrelliger sp.) was investigated. Smoked fish were irradiated with radiation doses of 2.0, 4.0, 6.0 and 8.0 kGy. The DMSO extracts of non-radiated and irradiated smoked fish were tested for mutagenicity using the Ames plate incorporation assay, host-mediated assay, and the micronucleus test. It was observed that gamma-irradiation did not induce any significant increase in the number of revertants of TA98, TA100 and TA104 as compared with the non-radiated smoked fish. Results of the host-mediated assay and the micronucleus test showed no difference in the mutagenic response of non-radiated and irradiated smoked fish. The results indicate that gamma-radiation does not introduce mutagens in smoked fish.
Dela Rosa
A M
AM
Atomic Research Division, Philippine Nuclear Research Institute, Diliman, Quezon City.
Banzon
R B
RB
D000818:Animals; D005399:Fishes; D005519:Food Preservation / Q000379:methods*; D005720:Gamma Rays; D008460:Meat / Q000528:radiation effects*; D051379:Mice; D015162:Micronucleus Tests; D009152:Mutagenicity Tests; D009154:Mutation / Q000187:drug effects*; D011839:Radiation, Ionizing; D012486:Salmonella typhimurium / Q000187:drug effects; D012906:Smoke
D012906:Smoke