Characterization of the HST-related FGF.6 gene, a new member of the fibroblast growth factor gene family.
By screening a mouse cosmid library with a human HST probe under reduced conditions of stringency, we isolated several positive clones. One of them was identified as a new member of the fibroblast growth factor gene family, and called FGF.6. The human FGF.6 gene was subsequently isolated and sequenced. The deduced amino-acid sequence exhibited 70% identity with the HST gene product over the C-terminal two-thirds of the putative protein. FGF.6 was mapped to chromosome 12 at band p13 by in situ hybridization. The cloned normal human gene was able to transform mouse NIH3T3 fibroblasts using both focus- and tumorigenicity-assays.
Marics
I
I
U.119 INSERM, Marseille, France.
Adelaide
J
J
Raybaud
F
F
Mattei
M G
MG
Coulier
F
F
Planche
J
J
de Lapeyriere
O
O
Birnbaum
D
D
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002471:Cell Transformation, Neoplastic; D002881:Chromosomes, Human, Pair 12; D003001:Cloning, Molecular; D051522:Fibroblast Growth Factor 6; D005346:Fibroblast Growth Factors / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
C496452:FGF6 protein, human; C486115:Fgf6 protein, mouse; D051522:Fibroblast Growth Factor 6; D011518:Proto-Oncogene Proteins; D005346:Fibroblast Growth Factors