[The Scl-70 antibody and its clinical significance].
In the review of the literature on the Scl-70 antigen, methods of detection of the Scl-70 antibody, the correlation between its presence and the clinical, genetic and immunological parameters are presented. It is emphasized that the Scl-70 antibody which is regarded as a marker of diffuse scleroderma, may have a prognostic significance. In indirect immunofluorescence on HEp-2 cells the Scl-70 antibody gives a characteristic pattern described as diffuse fine speckles with dotted nucleolus. A somewhat different but distinct staining pattern on hamster liver described by the authors was found to be characteristic of that antibody. The fluorescence associated with Scl-70 antibody is designated as nucleolar-reticular mixed staining with nucleolus predominating. It is suggested that hamster liver substrate which is easily available may replace the expensive HEp-2 cells for detecting Scl-70 antibody.
Kühn
G
G
Jarzabek-Chorzelska
M
M
Blaszczyk
M
M
Kolacinska-Strasz
Z
Z
Chorzelski
T P
TP
Jablonska
S
S
D000974:Antibodies, Antinuclear / Q000032:analysis*; D001324:Autoantigens / Q000276:immunology*; D004264:DNA Topoisomerases, Type I; D005455:Fluorescent Antibody Technique; D006801:Humans; D009687:Nuclear Proteins / Q000276:immunology*; D012595:Scleroderma, Systemic / Q000175:diagnosis* / Q000276:immunology
D000974:Antibodies, Antinuclear; D001324:Autoantigens; D009687:Nuclear Proteins; C048100:Scl 70 antigen, human; D004264:DNA Topoisomerases, Type I
Der Scl-70-Antikörper und seine klinische Bedeutung.