title: ELISA HIV testing and viral culture in the screening of corneal tissue for transplant from medical examiner cases.
abstract: The spread of AIDS and its association with a retrovirus, commonly called T-lymphotropic virus type III (HIV) has produced great concern among those involved in transplantation about the possibility for transmission of this disease through tissue and organ transplant. Isolation of the virus from conjunctiva and tears further heightened this concern for corneal transplants. This review of medical examiner (ME) cases, a population in which high numbers of transplant corneas are retrieved, has revealed positive serologic tests for HIV in serum from 5 cases in 205 screens. These represent potential donors based on other standard medical criteria available at the time of collection. This tissue was also submitted for viral culture, and virus was recovered from the corneal tissue in McCarey-Kaufman (MK) media. These findings and case reviews are discussed.
authors.affiliation: Transplant Services, University of Texas Southwestern Medical Center, Dallas 75235-9074.
authors.forename: E
authors.initials: E
authors.lastname: Heck
authors.forename: C
authors.initials: C
authors.lastname: Petty
authors.forename: A
authors.initials: A
authors.lastname: Palestine
authors.forename: M
authors.initials: M
authors.lastname: Luckenbach
authors.forename: S Z
authors.initials: SZ
authors.lastname: Salahuddin
authors.forename: R
authors.initials: R
authors.lastname: Nussenblatt
authors.forename: W
authors.initials: W
authors.lastname: Harris
authors.forename: C
authors.initials: C
authors.lastname: Baxter
authors.forename: J
authors.initials: J
authors.lastname: McCulley
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000175:diagnosis; D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D003315:Cornea / Q000382:microbiology; D016039:Corneal Transplantation; D003334:Coroners and Medical Examiners; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006678:HIV / Q000302:isolation & purification*; D006679:HIV Seropositivity / Q000175:diagnosis*; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D014019:Tissue Donors; D009927:Tissue and Organ Procurement / Q000592:standards; D014776:Virus Cultivation