Replication of measles virus in cultured human thymic epithelial cells.
Measles virus can replicate in cultures of both infantile and fetal human thymic epithelial cells. Virus-induced cytopathology including syncytium formation was first evident around 24 hr after viral inoculation of these cultures. At the same time, the cultures began to lose their characteristic thymus-like organizational structure. Viral antigens were detected in infected cells by indirect immunofluorescence, and the presence of progeny virions was demonstrated in culture fluids.
Numazaki
K
K
Department of Microbiology, McGill University, Montreal, Children's Hospital Research Institute, Canada.
Goldman
H
H
Wong
I
I
Wainberg
M A
MA
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002478:Cells, Cultured; D002675:Child, Preschool; D003588:Cytopathogenic Effect, Viral; D004848:Epithelium / Q000382:microbiology; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008459:Measles virus / Q000276:immunology / Q000502:physiology*; D010641:Phenotype; D013950:Thymus Gland / Q000196:embryology / Q000382:microbiology*; D014709:Vero Cells; D014779:Virus Replication
D000956:Antigens, Viral