title: The insulin-mimetic effect of vanadate is not correlated with insulin receptor tyrosine kinase activity nor phosphorylation in mouse diaphragm in vivo.
abstract: The in vivo administration of sodium orthovanadate stimulated the incorporation of [14C]glucose into [14C] glycogen, in a dose- and time-dependent manner, in mouse diaphragm. Activation of diaphragm insulin receptor was measured by exogenous tyrosine kinase activity and an antibody that recognizes a conformational change in the receptor beta-subunit upon autophosphorylation. Neither method detected insulin receptor activation by in vivo vanadate administration, suggesting that vanadate's insulin-mimetic effect on mouse diaphragm glycogenesis occurs at a site distal to the insulin receptor.
authors.affiliation: Department of Biochemical Endocrinology, Merck, Sharp, and Dohme Research Laboratories, Rahway, New Jerey 07065.
authors.forename: H V
authors.initials: HV
authors.lastname: Strout
authors.forename: P P
authors.initials: PP
authors.lastname: Vicario
authors.forename: R
authors.initials: R
authors.lastname: Saperstein
authors.forename: E E
authors.initials: EE
authors.lastname: Slater
mesh_terms: D000818:Animals; D003964:Diaphragm; D006003:Glycogen / Q000096:biosynthesis; D007328:Insulin / Q000502:physiology*; D051379:Mice; D009132:Muscles / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D010766:Phosphorylation; D011233:Precipitin Tests; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D011972:Receptor, Insulin / Q000378:metabolism*; D014638:Vanadates / Q000494:pharmacology*
chemical_list: D007328:Insulin; D014638:Vanadates; D006003:Glycogen; D011505:Protein-Tyrosine Kinases; D011972:Receptor, Insulin