Effects of photochemical donor pretreatment on the pattern of dog renal allograft infiltrating cells.
Kidneys were removed ten minutes after treating the donor with 1 mg of 8-methoxypsoralen per 1 kg b.w., during hypothermic preservation UVA-irradiated (intensity: 1.34 mJ/s) for 4 h and thereafter transplanted into ASDI dogs (n = 10). Fine needle aspiration biopsies were performed to analyze the inflammatory response. In comparison with untreated ASDI dogs (n = 9) PUVA pretreated renal grafts showed three important differences: 1. At the peak of the rejection process in the control group (day 8) the cellular infiltrate in the PUVA-pretreated kidneys was significantly diminished. 2. This reduction was caused by a significantly lower influx of monocytes/macrophages. 3. The peak of the cellular response in the PUVA group was delayed. These findings support the concept of reduced graft immunogenicity by pretransplant PUVA treatment.
Kaden
J
J
Division of Experimental Organ Transplantation, Friedrichshain Hospital, Berlin, German Democratic Republic.
Oesterwitz
H
H
Schirrow
R
R
D000818:Animals; D003404:Creatinine / Q000097:blood; D004285:Dogs; D005260:Female; D006084:Graft Rejection / Q000187:drug effects*; D007249:Inflammation / Q000473:pathology; D007668:Kidney / Q000276:immunology / Q000473:pathology; D016030:Kidney Transplantation; D008297:Male; D011701:PUVA Therapy; D014184:Transplantation, Homologous
D003404:Creatinine