Investigation on the enantiomeric impurity of epinephrine hydrochloride injections.
Epinephrine enantiomers were derived into diastereoisomers with the chiral reagent 2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosylisothiocyanate. The resolution was carried out on a C18 column. The Rs between (-)-R- and (+)-S-isomers was 2.3. The retention time could be changed by adding a proper amount of acetoinitrile into the mobile phase. The results showed that (+)-S-isomer in the epinephrine hydrochloride injections increased during the period of storage.
Yang
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ZY
Wuhan Municipal Institute for Drug Control, China.
Xu
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RZ
D002851:Chromatography, High Pressure Liquid; D004837:Epinephrine / Q000302:isolation & purification*; D007202:Indicators and Reagents; D017879:Isothiocyanates; D013237:Stereoisomerism; D013861:Thiocyanates
D007202:Indicators and Reagents; D017879:Isothiocyanates; D013861:Thiocyanates; C041943:2,3,4,6-tetra-O-acetylglucopyranosylisothiocyanate; D004837:Epinephrine