title: Improved performance of the second generation alpha 1-AGP columns: applications to the routine assay of plasma levels of alfuzosin hydrochloride.
abstract: Described is a direct enantioselective separation of the enantiomers of alfuzosin hydrochloride on the second generation alpha 1-AGP column which offers improved efficiency, shorter analysis, and improved stability with respect to the first generation columns. The method has been applied to the analysis of drug substance in rat plasma. This highly efficient extraction method and the use of fluorimetric detection result in selective and sensitive determination of the enantiomers. The analytical validation parameters demonstrate the applicability of this method to pharmacokinetic and metabolic studies.
authors.affiliation: Synthelabo Recherche Meudon la Foret, France.
authors.forename: A M
authors.initials: AM
authors.lastname: Krstulovic
authors.forename: J L
authors.initials: JL
authors.lastname: Vende
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D009961:Orosomucoid; D011799:Quinazolines / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism; D013552:Swine
chemical_list: D007202:Indicators and Reagents; D009961:Orosomucoid; D011799:Quinazolines; C047638:alfuzosin