A proposed mouse model for acute epididymitis provoked by genital serovar E, Chlamydia trachomatis.
This study was conducted to determine the efficacy of the male mouse as a model for epididymitis caused by human genital serovar E, Chlamydia trachomatis. C. trachomatis was reisolated from all tissues removed on Days 3, 5, and 7 post inoculation (pi). Although some infected epididymides removed on Days 10, 14, and 21 pi were positive, control tissues remained negative. Histopathology of tissues showed a heavy, mixed inflammatory infiltrate consisting of polymorphonuclear cells and lymphocytes. Serum antibody to C. trachomatis was detected in the infected mice only (titer greater than or equal to 1:32). Chlamydial inclusions and individual elementary bodies were confirmed by immunofluorescent and immunoperoxidase staining up to Day 7 pi. These data show that the male mouse is susceptible to C. trachomatis infection and is appropriate for studies dealing with the effect of C. trachomatis on male fertility.
Kuzan
F B
FB
Department of Obstetrics and Gynecology, University of Washington, Seattle 98195.
Patton
D L
DL
Allen
S M
SM
Kuo
C C
CC
D000208:Acute Disease; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D002690:Chlamydia Infections / Q000150:complications* / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D002692:Chlamydia trachomatis / Q000276:immunology; D004195:Disease Models, Animal; D004822:Epididymis / Q000382:microbiology / Q000473:pathology; D004823:Epididymitis / Q000150:complications* / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D005455:Fluorescent Antibody Technique; D007136:Immunoglobulins / Q000032:analysis; D007248:Infertility, Male / Q000209:etiology*; D008297:Male; D051379:Mice
D000907:Antibodies, Bacterial; D007136:Immunoglobulins