Differential expression of interferon alpha and beta induced with Newcastle disease virus in mouse macrophage cultures.
Stimulation of mouse macrophages with Newcastle disease virus (NDV) leads to a rapid and high interferon (IFN) response. The magnitude of this response is influenced by the mouse genotype. We have analysed NDV-induced IFN production at the protein and mRNA levels in two different populations of macrophages derived from 'high producer' C57BL/6 and 'low producer' BALB/c mice in vitro. The data indicate that bone marrow and peritoneal macrophages from both strains grown in the presence of L cell conditioned medium (CM) as a source of macrophage colony-stimulating factor 1 (M-CSF) or purified murine M-CSF produce 10- to 50-fold more IFN on a per cell basis than cultures of resident peritoneal macrophages. These differences were also found when steady state levels of IFN mRNA were analysed. Differential analysis for the ratios of IFN-alpha and IFN-beta showed that CM- or M-CSF-cultured macrophages produced equal amounts of both IFN species as determined by specific monoclonal antibodies and hybridization experiments using IFN-alpha and IFN-beta DNA probes, whereas resident peritoneal macrophages induced under identical conditions produced almost exclusively IFN-beta. This suggests a stimulating effect of M-CSF on IFN synthesis in NDV-induced cultures of mouse macrophages, which is in part due to additional activation of IFN-alpha gene expression.
Hoss
A
A
Institute of Virus Research, German Cancer Research Center, Heidelberg.
Zwarthoff
E C
EC
Zawatzky
R
R
D015088:2',5'-Oligoadenylate Synthetase / Q000096:biosynthesis; D000818:Animals; D001853:Bone Marrow; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D007370:Interferon Type I / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D007739:L Cells; D016173:Macrophage Colony-Stimulating Factor; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009522:Newcastle disease virus / Q000502:physiology*
D003115:Colony-Stimulating Factors; D007370:Interferon Type I; D016173:Macrophage Colony-Stimulating Factor; D015088:2',5'-Oligoadenylate Synthetase