title: Induction of anti-double stranded DNA antibodies in normal mice by immunization with bacterial DNA.
abstract: Because of recent observations suggesting that bacterial DNA is immunogenic, the induction in normal mice of antibodies to Escherichia coli (EC) dsDNA was investigated. BALB/c and C57BL/6 mice were immunized with dsEC or ds calf thymus (CT) DNA complexed to methylated BSA in adjuvant; antibody responses were measured by ELISA. In both strains, dsEC DNA immunization induced a much higher anti-dsDNA response to dsEC DNA than did dsCT DNA immunization. Neither immunized group showed an appreciable antibody response when tested on dsCT DNA. Anti-dsDNA antibodies were also demonstrated by ELISA using synthetic DNA duplexes as well as a filter binding assay using 3H-labeled dsEC DNA as Ag. These results suggest that bacterial dsDNA is immunogenic and that at least some anti-dsDNA specificities can arise by immunization.
authors.affiliation: Medical Research Service, Durham Veterans Administration Hospital, North Carolina 27710.
authors.forename: G S
authors.initials: GS
authors.lastname: Gilkeson
authors.forename: J P
authors.initials: JP
authors.lastname: Grudier
authors.forename: D G
authors.initials: DG
authors.lastname: Karounos
authors.forename: D S
authors.initials: DS
authors.lastname: Pisetsky
mesh_terms: D000818:Animals; D000974:Antibodies, Antinuclear / Q000032:analysis / Q000096:biosynthesis*; D000907:Antibodies, Bacterial / Q000032:analysis / Q000096:biosynthesis; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000276:immunology; D001666:Binding Sites, Antibody; D004247:DNA / Q000276:immunology*; D004269:DNA, Bacterial / Q000008:administration & dosage* / Q000276:immunology; D004926:Escherichia coli / Q000276:immunology; D007700:Kinetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008837:Micrococcus / Q000276:immunology
chemical_list: D000974:Antibodies, Antinuclear; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D004269:DNA, Bacterial; D004247:DNA