title: Clusters of 43-kDa protein are absent from genetic variants of C2 muscle cells with reduced acetylcholine receptor expression.
abstract: Genetic variants of the C2 muscle cell line were used to investigate the relation between acetylcholine receptor (AChR) clustering and clustering of the 43-kDa protein. Two variants that express severely reduced amounts of the alpha subunit of the AChR and consequently lack AChR clusters were found also to lack clusters of the 43-kDa protein. The amount of 43-kDa protein in the variants measured by immunoassay was reduced to about one-third the levels found in wild-type cells. The beta subunit of the AChR was reduced to a similar extent. Northern blot analysis showed that neither the 43-kDa protein mRNA nor the beta subunit mRNA was reduced in the variants. Taken together, these results suggest that the amounts of beta subunit and 43-kDa protein may be regulated coordinately by a post-transcriptional mechanism.
authors.affiliation: Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03756.
authors.forename: W J
authors.initials: WJ
authors.lastname: LaRochelle
authors.forename: E
authors.initials: E
authors.lastname: Ralston
authors.forename: J R
authors.initials: JR
authors.lastname: Forsayeth
authors.forename: S C
authors.initials: SC
authors.lastname: Froehner
authors.forename: Z W
authors.initials: ZW
authors.lastname: Hall
mesh_terms: D000818:Animals; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000502:physiology*; D051379:Mice; D008970:Molecular Weight; D009024:Morphogenesis; D009132:Muscles / Q000648:ultrastructure*; D012333:RNA, Messenger / Q000502:physiology; D011978:Receptors, Nicotinic / Q000648:ultrastructure*; D014404:Tubulin / Q000235:genetics
chemical_list: D046911:Macromolecular Substances; D008565:Membrane Proteins; D012333:RNA, Messenger; D011978:Receptors, Nicotinic; D014404:Tubulin