title: The Pseudomonas oleovorans alkBAC operon encodes two structurally related rubredoxins and an aldehyde dehydrogenase.
abstract: The Pseudomonas oleovorans alkBAC operon encodes seven proteins, of which at least three are involved in alkane hydroxylase (alkBA) and alkanol dehydrogenase (alkC) activities. We have determined the nucleotide sequence of the 2.5-kilobase pair alkA region and analyzed the role of its translation products in alkane oxidation. The alkA region contains three coding sequences, encoding two related rubredoxins (alkF and alkG) of 14- and 18-kDa molecular mass and a 52-kDa aldehyde dehydrogenase (alkH). Deletion analysis indicated that neither the 14-kDa alkF gene product (rubredoxin 1) nor the amino-terminal part of the 18-kDa alkG gene product (rubredoxin 2) is required for alkane hydroxylase activity in vivo. The product of the alkH cistron restores growth of a P. oleovorans aldehyde dehydrogenase mutant on aliphatic alcohols and aldehydes. Its amino acid sequence shows considerable homology to previously characterized aldehyde dehydrogenases from mammalian and fungal origin. The nucleotide composition of the alk genes (47% G + C) differs considerably from the G + C content of the P. oleovorans genome suggesting that the alk regulon may originate from an unrelated organism.
authors.affiliation: Groningen Biotechnology Center, University of Groningen, The Netherlands.
authors.forename: M
authors.initials: M
authors.lastname: Kok
authors.forename: R
authors.initials: R
authors.lastname: Oldenhuis
authors.forename: M P
authors.initials: MP
authors.lastname: van der Linden
authors.forename: C H
authors.initials: CH
authors.lastname: Meulenberg
authors.forename: J
authors.initials: J
authors.lastname: Kingma
authors.forename: B
authors.initials: B
authors.lastname: Witholt
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D042926:Cytochrome P-450 CYP4A; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005288:Ferredoxins / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D005838:Genotype; D006899:Mixed Function Oxygenases / Q000235:genetics; D008969:Molecular Sequence Data; D009876:Operon; D010641:Phenotype; D010957:Plasmids; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics*; D015183:Restriction Mapping; D012416:Rubredoxins / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D005288:Ferredoxins; D012416:Rubredoxins; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D042926:Cytochrome P-450 CYP4A; D000444:Aldehyde Dehydrogenase