Separation and quantitation of (R)- and (S)-atenolol in human plasma and urine using an alpha 1-AGP column.
A method for the determination of (R)- and (S)-atenolol in human plasma and urine is described. The enantiomers of atenolol are extracted into dichloromethane containing 3% heptafluorobutanol followed by acetylation with acetic anhydride at 60 degrees C for 2 h. The acetylated enantiomers were separated on a chiral alpha 1-AGP column. Quantitation was performed using fluorescence detection. A phosphate buffer pH 7.1 (0.01 M phosphate) containing 0.25% (v/v) acetonitrile was used as mobile phase. The described procedure allows the detection of less than 6 ng of each enantiomer in 1 ml plasma. The relative standard deviation is 4.4% at 30 ng/ml of each enantiomer in plasma. The plasma concentration of (R)- and (S)-atenolol did not differ significantly in two subjects who received a single tablet of racemic atenolol. The R/S ratio of atenolol in urine was approximately 1.
Enquist
M
M
Apoteksbolaget AB, Department of Biomedicine, Stockholm, Sweden.
Hermansson
J
J
D001262:Atenolol / Q000032:analysis* / Q000097:blood / Q000652:urine; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D004206:Disopyramide / Q000378:metabolism; D006801:Humans; D008297:Male; D009961:Orosomucoid; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism
D009961:Orosomucoid; D001262:Atenolol; D004206:Disopyramide