Heparin cofactor IIOslo. Mutation of Arg-189 to His decreases the affinity for dermatan sulfate.
Heparin and dermatan sulfate increase the rate of inhibition of thrombin by heparin cofactor II (HCII) approximately 1000-fold by providing a catalytic template to which both the inhibitor and the proteinase bind. A variant form of HCII that binds heparin but not dermatan sulfate has been described recently in two heterozygous individuals (Andersson, T.R., Larsen, M.L., and Abildgaard, U. (1987) Thromb. Res. 47, 243-248). We have now purified the variant HCII (designated HCIIOslo) from the plasma of ne of these individuals. HCIIOslo or normal HCII (11 nM) was incubated with thrombin (9 nM) for 1 min in the presence of heparin or dermatan sulfate. Fifty percent inhibition of thrombin occurred at 26 micrograms/ml dermatan sulfate with normal HCII and greater than 1600 micrograms/ml dermatan sulfate with HCIIOslo. In contrast, inhibition of thrombin occurred at a similar concentration of heparin (1.0-1.5 micrograms/ml) with both inhibitors. To identify the mutation in HCIIOslo, DNA fragments encoding the N-terminal 220 amino acid residues of HCII were amplified from leukocyte DNA by the Taq DNA polymerase chain reaction and both alleles were cloned. A point mutation (G----A) resulting in substitution of His for Arg-189 was found in one allele. The same mutation was constructed in the cDNA of native HCII by oligonucleotide-directed mutagenesis and expressed in Escherichia coli. The recombinant HCIIHis-189 reacted with thrombin in the presence of heparin but not dermatan sulfate, confirming that this mutation is responsible for the functional abnormality in HCIIOslo.
Blinder
M A
MA
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110.
Andersson
T R
TR
Abildgaard
U
U
Tollefsen
D M
DM
D000595:Amino Acid Sequence; D001120:Arginine / Q000235:genetics; D002807:Chondroitin / Q000031:analogs & derivatives*; D003001:Cloning, Molecular; D003871:Dermatan Sulfate / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D005784:Gene Amplification; D006023:Glycoproteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D015844:Heparin Cofactor II; D006639:Histidine / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D011994:Recombinant Proteins / Q000235:genetics
D006023:Glycoproteins; D011994:Recombinant Proteins; D003871:Dermatan Sulfate; D006639:Histidine; D015844:Heparin Cofactor II; D002807:Chondroitin; D001120:Arginine