[Mass culture of LAK cells by a hollow-fiber bioreactor system].
We attempted to culture LAK cells by the use of the hollow-fiber bioreactor system, "Acusyst-P". The number of LAK cells increased by 30-40 times. The majority of LAK cells cultured by this hollow fiber system originated in T cell. LAK cells cultured by hollow-fiber system were different from LAK cells statically cultured in their cytotoxicity and change of phenotype. We obtained LAK cells more efficiently and safely which have higher viability and cytotoxicity by the use of hollow-fiber bioreactor system than by static culture.
Tanji
Y
Y
Dept. of Oncologic Surgery, Osaka University.
Tanaka
T
T
Kimoto
Y
Y
Fujiwara
A
A
Fujita
M
M
Taguchi
T
T
D003584:Cytological Techniques; D006801:Humans; D007694:Killer Cells, Natural; D008213:Lymphocyte Activation; D008222:Lymphokines
D008222:Lymphokines