Constitutive mutants in the yeast pheromone response: ordered function of the gene products.
The alpha factor pheromone inhibits the division of yeast a cells. A general method was developed for isolating mutants that exhibit constitutive activation of the pheromone response pathway. A dominant allele of the STE4 locus was recovered in addition to recessive mutations in the SCG1 gene. SCG1 and STE4 are known to encode G alpha and G beta homologs, respectively. Analysis of double mutants suggests that the STE4 gene product functions after the SCG1 product but before the STE5 product.
Blinder
D
D
Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester 01655.
Bouvier
S
S
Jenness
D D
DD
D003433:Crosses, Genetic; D005796:Genes; D005799:Genes, Dominant; D005800:Genes, Fungal; D005808:Genes, Recessive; D005838:Genotype; D000072235:Mating Factor; D009154:Mutation; D010455:Peptides / Q000235:genetics* / Q000502:physiology; D010641:Phenotype; D010675:Pheromones / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000502:physiology
D010455:Peptides; D010675:Pheromones; D000072235:Mating Factor