Phosphorothioates in molecular biology.
The observation that phosphorothioate analogues of the nucleoside triphosphates are substrates for DNA- and RNA-polymerases has proven a boon for the molecular biologist. As these phosphorothioate-containing polymers are stable to degradation by nucleases and the sulfur atom confers many favourable chemical properties, several applications in molecular biology have been developed, including new methods for site-directed mutagenesis and DNA sequencing.
Eckstein
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Gish
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D004247:DNA / Q000032:analysis; D008967:Molecular Biology; D013873:Thionucleotides / Q000378:metabolism*
D013873:Thionucleotides; D004247:DNA