Association of v-myc protein with chromatin.
The subnuclear distribution of proteins encoded by v-myb and v-myc was analysed in a cell-line of AMV-transformed chicken myeloblasts superinfected by the myc-containing retrovirus MC29. p45v-myb and p110gag-myc, co-expressed in these cells, were released in similar fashion when nuclei were treated with salt or DNAase. Analysis of nucleoprotein complexes extracted from nuclease-treated nuclei shows that p45v-myb and p110gag-myc are associated with a chromatin fraction of enhanced nuclease sensitivity. v-myb and v-myc proteins thus share the same subnuclear location and apparently interact directly with the cellular DNA.
Klempnauer
K H
KH
Zentrum für Molekulare Biologie, Universität Heidelberg, Federal Republic of Germany.
D000818:Animals; D009189:Avian Myeloblastosis Virus / Q000235:genetics; D002461:Cell Line, Transformed; D002499:Centrifugation, Density Gradient; D002843:Chromatin / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis; D016274:Oncogene Protein p55(v-myc); D020626:Oncogene Proteins v-myb; D012191:Retroviridae Proteins / Q000032:analysis*
D002843:Chromatin; D004273:DNA, Neoplasm; D016274:Oncogene Protein p55(v-myc); D020626:Oncogene Proteins v-myb; D012191:Retroviridae Proteins