Characterization of leukocyte enzymes involved in the release of amino acids in incubated blood cell lysates.
The neutral leukocyte proteases involved in the release of free amino acids in incubation mixtures of blood cell lysates and of human serum albumin with leukocyte lysates were characterized by several techniques including 1H nuclear magnetic resonance spectroscopy, electrophoresis, high performance liquid chromatography, gel filtration, amino acid analysis, and NH2-terminal analysis. The data suggested that the enzymes which contributed significantly to the extensive protein hydrolysis observed were two endopeptidases and three exopeptidases. Identification and analysis of the products obtained in incubation mixtures of human serum albumin with elastase and leucine aminopeptidase were compared with the products obtained in incubation mixtures of the protein with leukocyte lysates.
Mendz
G L
GL
Department of Biochemistry, University of Sydney, New South Wales, Australia.
McCall
M N
MN
Kuchel
P W
PW
D000596:Amino Acids / Q000097:blood*; D002122:Calcium Chloride / Q000494:pharmacology; D003600:Cytosol / Q000201:enzymology; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D007931:Leucyl Aminopeptidase / Q000378:metabolism; D007962:Leukocytes / Q000201:enzymology*; D008274:Magnesium / Q000494:pharmacology; D015636:Magnesium Chloride; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008861:Microsomes / Q000201:enzymology; D010196:Pancreatic Elastase / Q000378:metabolism; D010447:Peptide Hydrolases / Q000097:blood*; D011480:Protease Inhibitors / Q000494:pharmacology; D012709:Serum Albumin / Q000378:metabolism
D000596:Amino Acids; D011480:Protease Inhibitors; D012709:Serum Albumin; D015636:Magnesium Chloride; D010447:Peptide Hydrolases; D007931:Leucyl Aminopeptidase; D010196:Pancreatic Elastase; D008274:Magnesium; D002122:Calcium Chloride