Antigen detected on normal human B lymphocytes by BR31 monoclonal antibody--the nature of the antigen and its functional properties.
Monoclonal BR31 antibody reacts with a subpopulation of B lymphocytes from normal human blood and tissue. The paper shows that the antigen recognized by BR31 is a protein or at least depends on protein synthesis for its expression. BR31 mAb does not cause spontaneous proliferation of B cells. However, it greatly enhances proliferation of normal tonsil B cells in response to SAC and PMA. No costimulation was observed when the cells were cultured in the presence of BR31 mAb and anti-Ig or BCGF. It was also found that there is a subpopulation of B cells which is able to proliferate with SAC or PMA only in the presence of BR31 mAb.
Skibiński
G
G
Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw.
Wieczorek
Z
Z
D000911:Antibodies, Monoclonal; D000944:Antigens, Differentiation, B-Lymphocyte / Q000276:immunology* / Q000502:physiology; D002478:Cells, Cultured; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*
D000911:Antibodies, Monoclonal; D000944:Antigens, Differentiation, B-Lymphocyte