Effector cells involved in nonspecific and antibody-dependent mechanisms directed against Plasmodium falciparum blood stages in vitro.
We have evaluated in in vitro conditions the possible cooperative effect of antimalarial antibodies with several human blood cell types. When used alone, immunoglobulin G from African adults who had reached a state of premunition against malaria was found to have no or very limited direct effect on invasion and multiplication of P. falciparum asexual blood stages. In contrast, these antibodies induced a marked specific inhibition of parasite growth in the presence of normal blood monocytes, and the inhibition did not appear to be strain dependent. No similar antibody-dependent cellular inhibitory effect was found using human blood polymorphonuclear leukocytes, lymphocytes, platelets, or adherent spleen cells. However, these cells could all exert in vitro some non-antibody-dependent inhibitory effect when present at high effector/target cell ratios.
Lunel
F
F
Faculté de Médecine Paris VI, France.
Druilhe
P
P
D000328:Adult; D000818:Animals; D000913:Antibodies, Protozoan / Q000502:physiology*; D000920:Antibody-Dependent Cell Cytotoxicity; D001792:Blood Platelets / Q000276:immunology / Q000469:parasitology; D002448:Cell Adhesion; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D006131:Growth Inhibitors / Q000502:physiology; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007074:Immunoglobulin G / Q000502:physiology; D008214:Lymphocytes / Q000276:immunology / Q000469:parasitology; D008288:Malaria / Q000097:blood* / Q000276:immunology / Q000469:parasitology; D009000:Monocytes / Q000276:immunology / Q000469:parasitology; D009504:Neutrophils / Q000276:immunology / Q000469:parasitology; D010963:Plasmodium falciparum / Q000254:growth & development / Q000276:immunology*; D013154:Spleen / Q000276:immunology / Q000469:parasitology
D000913:Antibodies, Protozoan; D006131:Growth Inhibitors; D007106:Immune Sera; D007074:Immunoglobulin G