Hemagglutinating activity of the B subunit(s) of the heat-labile enterotoxin isolated from chicken enterotoxigenic Escherichia coli.
The hemagglutinating activity of the B subunit(s) of the heat-labile enterotoxin (LTc-B) produced by chicken enterotoxigenic Escherichia coli was studied by hemagglutination and hemagglutination inhibition. No or weak hemagglutination of intact human erythrocytes was found by the LTc-B at the highest concentration used, whereas strong hemagglutination of both neuraminidase- and pronase-treated human erythrocytes was found. Enhancement in hemagglutination of treated human erythrocytes induced by the LTc-B was over 2 to 120-fold for type A and B erythrocytes and over 8-fold for type O erythrocytes, respectively. With intact and treated sheep erythrocytes, on the other hand, no hemagglutination was found by the LTc-B at the highest concentration used. Hemagglutination of pronase-treated human type B erythrocytes by the LTc-B was inhibited by methyl-alpha-D-galactopyranoside, galactose, melibiose, hog A + H, asialo-bovine salivary mucin and asialo-thyroglobulin among mono-, di- and polysaccharides and glycoproteins used as inhibitors. These results suggest that the LTc-B is a galactose-specific bacterial lectin.
Sugii
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Department of Serology and Immunology, School of Medical Technology, Kitasato University, Kanagawa, Japan.
Tsuji
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D000818:Animals; D001427:Bacterial Toxins / Q000302:isolation & purification*; D002645:Chickens / Q000382:microbiology; D004768:Enterotoxins / Q000302:isolation & purification*; D004912:Erythrocytes / Q000187:drug effects / Q000276:immunology; D004926:Escherichia coli / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification; D029968:Escherichia coli Proteins; D005677:G(M1) Ganglioside / Q000494:pharmacology; D006386:Hemagglutination Tests; D006388:Hemagglutinins / Q000302:isolation & purification*; D006801:Humans; D066298:In Vitro Techniques
D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D006388:Hemagglutinins; D005677:G(M1) Ganglioside; C027538:heat-labile enterotoxin, E coli