title: A monoclonal antibody (BL2-10D1) reacting with a bladder-cancer-associated antigen.
abstract: A hybridoma cell line secreting an IgM monoclonal antibody (MAb) was produced after immunizing a mouse with RT4 cells and a crude suspension of human bladder carcinoma cells (WHO grades II and III TCC). This MAb reacted with RT4 target cells derived from a human transitional bladder cancer but failed to react with a majority of non-bladder cancer cell lines. Immunohistological studies indicate that this MAb reacts inconstantly with normal bladder: in positive cases only a few superficial cells (5% to 10% umbrella cells) are stained but not intermediate or basal cells of the urothelium. This MAb was evaluated on 118 tumors: it reacted with tumor tissue in a majority of grade I (79.5%) and grade II papillary TCC (77.3%), less with grade III papillary TCC (45%) and very rarely with invasive non-papillary TCC (14%). In cases of flat lesions a strong reactivity of superficial, intermediate and/or basal layer cells was observed in 50% of moderate and severe dysplasia and in all cell layers of carcinomas in situ (CIS)(9/9).
authors.affiliation: Centre Léon Bérard, Lyon, France.
authors.forename: A
authors.initials: A
authors.lastname: Longin
authors.forename: A
authors.initials: A
authors.lastname: Hijazi
authors.forename: N
authors.initials: N
authors.lastname: Berger-Dutrieux
authors.forename: G
authors.initials: G
authors.lastname: Escourrou
authors.forename: R
authors.initials: R
authors.lastname: Bouvier
authors.forename: G
authors.initials: G
authors.lastname: Richer
authors.forename: I
authors.initials: I
authors.lastname: Mironneau
authors.forename: B
authors.initials: B
authors.lastname: Fontanière
authors.forename: M
authors.initials: M
authors.lastname: Devonec
authors.forename: J C
authors.initials: JC
authors.lastname: Laurent
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D000912:Antibodies, Neoplasm / Q000276:immunology*; D002278:Carcinoma in Situ / Q000276:immunology*; D002295:Carcinoma, Transitional Cell / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D014407:Tumor Cells, Cultured / Q000276:immunology; D001743:Urinary Bladder / Q000276:immunology; D001749:Urinary Bladder Neoplasms / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm