title: Evaluation of fluorescence polarization immunoassay for determination of cyclosporin in plasma.
abstract: The fluorescence polarization immunoassay (FPIA) method for determination of cyclosporin in plasma was evaluated and compared with the high-performance liquid chromatography (HPLC) and the radioimmunoassay (RIA) methods. The coefficients of variation for the within-run and between-run precision were less than 5 and less than 8%, respectively, for samples ranging in concentration from 50 to 600 ng/ml. Recoveries were determined by adding cyclosporin at concentrations from 25 to 1,000 ng/ml to patient plasma; they were, on average, 98.5%. The calibration curve was stable throughout a 10-week study period. There was no clinically significant interference due to hemolysis, icterus, lipemia, or other commonly used drugs. There was considerable variation of the ratio of the FPIA result to the HPLC result, whereas there was a good correlation between the FPIA and the RIA results (r = 0.975, n = 25, y = 1.2x - 36.4), when evaluated using specimens from renal transplant patients receiving cyclosporin orally. It was concluded that the FPIA is an appropriate, rapid method for patient cyclosporin analysis in plasma and serves as a practical alternative to the RIA.
authors.affiliation: Hospital Pharmacy, Shiga University of Medical Science, Ohtsu, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Hayashi
authors.forename: N
authors.initials: N
authors.lastname: Shibata
authors.forename: T
authors.initials: T
authors.lastname: Minouchi
authors.forename: H
authors.initials: H
authors.lastname: Shibata
authors.forename: T
authors.initials: T
authors.lastname: Ono
authors.forename: H
authors.initials: H
authors.lastname: Shimakawa
mesh_terms: D000293:Adolescent; D000328:Adult; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D003524:Cyclosporins / Q000097:blood*; D005454:Fluorescence Polarization; D006461:Hemolysis; D006801:Humans; D007118:Immunoassay; D007565:Jaundice / Q000097:blood; D016030:Kidney Transplantation; D008875:Middle Aged; D011863:Radioimmunoassay; D014280:Triglycerides / Q000097:blood
chemical_list: D003524:Cyclosporins; D014280:Triglycerides