Identification and purification of a Saccharomyces cerevisiae protein with the DNA binding specificity of mammalian activating transcription factor.
Activating transcription factor (ATF) is a mammalian transcriptional activator, which is involved in the expression of many viral E1a-inducible and cellular cAMP-inducible genes. Here we identify from the yeast Saccharomyces cerevisiae a previously uncharacterized protein whose DNA binding specificity is like mammalian ATF. We purify this protein (yATF) and show that it is a 66-kDa polypeptide. Finally, we demonstrate that a mammalian ATF site can function as an upstream activating sequence in S. cerevisiae. Taken together, our results suggest that yATF is a previously uncharacterized S. cerevisiae transcriptional activator.
Lin
Y S
YS
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138.
Green
M R
MR
D051696:Activating Transcription Factors; D000818:Animals; D001798:Blood Proteins / Q000302:isolation & purification* / Q000378:metabolism; D003432:Cross-Linking Reagents; D005786:Gene Expression Regulation; D005800:Genes, Fungal; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity; D014157:Transcription Factors / Q000302:isolation & purification* / Q000378:metabolism; D014466:Ultraviolet Rays
D051696:Activating Transcription Factors; D001798:Blood Proteins; D003432:Cross-Linking Reagents; D014157:Transcription Factors