title: Production of dodecanedioic acid from n-dodecane by yeasts.
abstract: In order to develop a process for production of dodecanedioic acid (DC-12) as starting materials for the chemical industry, a n-dodecane assimilating and DC-12-producing yeast was isolated from Taiwan soil. The taxonomical characteristics of this newly isolated yeast were examined and it was identified as Candida tropicalis NTU-512. The newly isolated strain was improved successively by NTG mutagenesis. A high potency DC-12-producing mutant 91 which showed slight growth both in DC-12 and n-dodecane enrichment media was isolated. The DC-12 productivity of mutant 91 reached 3,326 mg/I by shaking culture at 30 degrees C for 72 h. This figure is 2.5-fold that of the parent strain (1,310 mg/I).
authors.affiliation: Institute of Agricultural Chemistry, National Taiwan University, Taipei, ROC.
authors.forename: W H
authors.initials: WH
authors.lastname: Liu
authors.forename: C K
authors.initials: CK
authors.lastname: Kuo
mesh_terms: D000473:Alkanes / Q000378:metabolism*; D002175:Candida / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D003998:Dicarboxylic Acids / Q000032:analysis / Q000378:metabolism*; D008769:Methylnitronitrosoguanidine; D009154:Mutation
chemical_list: D000473:Alkanes; D003998:Dicarboxylic Acids; C007548:n-dodecane; D008769:Methylnitronitrosoguanidine; C036836:dodecanedioic acid