Hyperthermic effects in vitro on the peripheral lymphocytes isolated from three cases of malignant lymphoma/leukemia.
We have carried out in vitro studies of hyperthermic effects on the viability, blastogenesis, and ultrastructural changes of atypical lymphocytes isolated from the peripheral blood of three patients with malignant lymphoma/leukemia. One was lymphocytic lymphoma/leukemia (T-cell origin), and the others were adult T-cell lymphoma/leukemia (both acute and smoldering type). In the cases of adult T-cell lymphoma/leukemia, the peripheral lymphocytes were found to be more sensitive to hyperthermia than those of non-ATL patients in terms of trypan-blue exclusion ability and blastogenesis ability with concanavalin A challenge. When heated under sublethal conditions, lymphocytes of both ATL and non-ATL patients exhibited the following characteristics: production of multilayered nuclear membranes, increase in vesicles (lysosomes), swelling of mitochondria, and cytoplasmic vacuolization.
Nakayama
J
J
Higuchi
R
R
Kabemura
M
M
Kiryu
H
H
Takenaka
A
A
Hori
Y
Y
D000328:Adult; D002454:Cell Differentiation; D002470:Cell Survival; D003208:Concanavalin A / Q000276:immunology; D006358:Hot Temperature; D006801:Humans; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000097:blood*; D008214:Lymphocytes / Q000502:physiology* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged
D003208:Concanavalin A