An enzyme immunoassay for the determination of lipase in human duodenal fluid.
The application of a commercially available enzyme immunoassay method for the determination of lipase concentration in human duodenal fluid is reported. Variations in the test procedure have been investigated. A sample incubation and a conjugate incubation of 1 h at 37 degrees C are necessary. There is substantial agreement between the determination of lipase in duodenal fluid measured according to this enzyme immunoassay and a turbidimetric method. The output of pancreatic lipase, alpha-amylase, and trypsin in four healthy volunteers during infusion of stepwise increasing doses of cholecystokinin was measured. A parallel increase of the output of these three enzymes has been observed, and there is a correlation between the activity concentrations of alpha-amylase and trypsin and the content of lipase in the various samples. This study shows that lipase in duodenal fluid can be easily and reliably determined with this enzyme immunoassay.
Hafkenscheid
J C
JC
Department of Internal Medicine, St. Radboud Ziekenhuis, University of Nijmegen, The Netherlands.
Hessels
M
M
Kerstens
P J
PJ
Lamers
C B
CB
D000328:Adult; D001826:Body Fluids / Q000201:enzymology*; D002766:Cholecystokinin / Q000008:administration & dosage / Q000494:pharmacology; D004386:Duodenum / Q000201:enzymology*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques / Q000592:standards*; D007262:Infusions, Intravenous; D008049:Lipase / Q000032:analysis*; D008297:Male; D009391:Nephelometry and Turbidimetry / Q000379:methods; D014357:Trypsin / Q000378:metabolism; D000516:alpha-Amylases / Q000378:metabolism
D002766:Cholecystokinin; D008049:Lipase; D000516:alpha-Amylases; D014357:Trypsin