The La antigen shuttles between the nucleus and the cytoplasm in CV-1 cells.
Recently we established a monoclonal antibody against the La-protein (Bachmann et al., Proc. Natl. Acad. Sci. USA, 83, 7770, 1986). The antibody gives a nuclear speckled type staining and, in addition, a perinuclear cytoplasmic staining on cultured cells in immunofluorescence microscopy. After inhibition of RNA synthesis the La-protein is transported into the cytoplasm. After prolonged inhibition it returns into the nucleus forming large growing speckles. The transport into the nucleus apparently depends on glycosylation.
Bachmann
M
M
Institut für Physiologische Chemie, Universität, Mainz, FRG.
Pfeifer
K
K
Schröder
H C
HC
Müller
W E
WE
D000818:Animals; D000911:Antibodies, Monoclonal; D001324:Autoantigens / Q000235:genetics* / Q000302:isolation & purification; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D008970:Molecular Weight; D011499:Protein Processing, Post-Translational; D012261:Ribonucleoproteins; D014157:Transcription Factors / Q000235:genetics; D000097585:SS-B Antigen
D000911:Antibodies, Monoclonal; D001324:Autoantigens; D012261:Ribonucleoproteins; D014157:Transcription Factors; D000097585:SS-B Antigen