Kallikrein-related protease in the rat ventral prostate: cDNA cloning and androgen regulation.
A kallikrein-related protease was purified from rat ventral prostate cytosol by means of DEAE-Sepharose chromatography, followed by gel filtration on Sephadex G-100 and CM-cellulose chromatography. Antibodies raised in rabbits against the purified protease recognize two bands on immunoblots of prostatic cytosol: a 31,000 Da band and an 18,000 Da band, which constitutes a proteolytic breakdown product of the former. The corresponding cDNA was isolated from a prostatic cDNA library, inserted in a lambda gt11 vector, using immunodetection for screening and identified as encoding a kallikrein- and tonin-related protease. Castration resulted in a marked decrease of the level of the protease and its mRNA, whereas administration of androgens to castrated animals resulted in marked stimulation. These data support the hypothesis that this protease is a member of a cluster of proteins, that are regulated in parallel by androgens in prostatic epithelial cells.
Winderickx
J
J
Laboratorium voor Experimentele Geneeskunde en Endocrinologie (LEGENDO), Faculteit Geneeskunde, K.U. Leuven, Belgium.
Swinnen
K
K
Van Dijck
P
P
Verhoeven
G
G
Heyns
W
W
D000595:Amino Acid Sequence; D000728:Androgens / Q000494:pharmacology; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D015151:Immunoblotting / Q000379:methods; D007124:Immunoenzyme Techniques; D007610:Kallikreins / Q000235:genetics / Q000302:isolation & purification*; D008297:Male; D008969:Molecular Sequence Data; D009919:Orchiectomy; D011467:Prostate / Q000201:enzymology*; D012333:RNA, Messenger / Q000187:drug effects; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains
D000728:Androgens; D012333:RNA, Messenger; D007610:Kallikreins