Construction of an ordered cosmid collection of the Escherichia coli K-12 W3110 chromosome.
A cosmid library of the Escherichia coli K-12 W3110 chromosome was constructed in which clones were assigned to locations on the chromosome map by hybridization and genetic marker complementation tests. Approximately 70% of the genome was represented by this library. The identified clones can be maintained in the homologous system and would facilitate genetic studies of E. coli.
Tabata
S
S
Department of Biology, Faculty of Science, Nagoya University, Japan.
Higashitani
A
A
Takanami
M
M
Akiyama
K
K
Kohara
Y
Y
Nishimura
Y
Y
Nishimura
A
A
Yasuda
S
S
Hirota
Y
Y
D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D003360:Cosmids; D004926:Escherichia coli / Q000235:genetics*; D005838:Genotype