Inhibition of proteinases with recombinant eglin C during experimental Escherichia coli septicemia in the pig.
Administration of the proteinase inhibitor eglin C reduces the symptoms of capillary leakage in a porcine model of septic shock. This was assessed by measurements of blood pressure, plasma protein concentration, hematocrit, and duration of urine production. Eglin C plasma levels around 1.2 microM resulted from a dose of 1.9 mg.kg-1.h-1 for 4 h and were therapeutically effective. A higher dose of eglin C (7.7 mg.kg-1.h-1 for 4 h) induced levels of approximately 5.6 microM in plasma and was not superior. This observation indicates that lysosomal proteases from neutrophils or degranulation of mast cells play a crucial role in the increase of capillary permeability during septicemia.
Siebeck
M
M
Chirurgische Klinik Innenstadt, Ludwig-Maximilians-Universität München, BRD.
Hoffmann
H
H
Jochum
M
M
Fritz
H
H
D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D001798:Blood Proteins / Q000032:analysis; D004927:Escherichia coli Infections / Q000503:physiopathology*; D006400:Hematocrit; D007668:Kidney / Q000187:drug effects / Q000502:physiology; D011480:Protease Inhibitors / Q000494:pharmacology*; D011506:Proteins / Q000494:pharmacology*; D018805:Sepsis / Q000503:physiopathology*; D015843:Serpins; D013552:Swine
D001798:Blood Proteins; D011480:Protease Inhibitors; D011506:Proteins; D015843:Serpins; C028656:eglin proteinase inhibitors