Evidence of coordinate regulation of virulence in Salmonella typhimurium involving the rsk element of the 95-kilobase plasmid.
Integration of the Salmonella typhimurium virulence plasmid into the chromosome reduces mouse virulence, serum resistance, and HeLa cell adhesion-invasion while prolonging lag time in minimal medium. The proposed virulence plasmid regulatory element, rsk, partially restores virulence and fully restores the other three phenotypes to wild-type levels. Plasmid curing reduces virulence without affecting the other phenotypes. rsk has no apparent effect on the cured strain.
Vandenbosch
J L
JL
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109.
Kurlandsky
D R
DR
Urdangaray
R
R
Jones
G W
GW
D000818:Animals; D001482:Base Composition; D005798:Genes, Bacterial; D051379:Mice; D010957:Plasmids; D012045:Regulatory Sequences, Nucleic Acid; D012486:Salmonella typhimurium / Q000235:genetics / Q000472:pathogenicity*; D014774:Virulence