title: Presence of translational inhibitory activity in partially purified extracts from two Petrocoptis species.
abstract: The presence of translational inhibitory activity in partially purified extracts from several paleoendemisms from Spain was investigated. The precipitates from 40-80% (NH4)2SO4 fraction from Petrocoptis glaucifolia and Petrocoptis grandiflora displayed a strong inhibitory activity on the protein synthesis of cell-free extracts from rat liver, rabbit reticulocytes and yeast and to a much lower extent on the protein synthesis in isolated rat liver cells. The inhibitors seem to be proteins since they were precipitated by high salt concentrations, were non-dialysable and were inactivated by heat. Since the partially purified extracts did not show unspecific RNA-A or protease activities, the active compounds can be considered to belong to the plant ribosome-inactivating proteins.
authors.forename: J M
authors.initials: JM
authors.lastname: Ferreras
authors.forename: J M
authors.initials: JM
authors.lastname: Diez
authors.forename: R
authors.initials: R
authors.lastname: Iglesias
authors.forename: M J
authors.initials: MJ
authors.lastname: Merino
authors.forename: T
authors.initials: T
authors.lastname: Girbés
mesh_terms: D010936:Plant Extracts / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects*; D011500:Protein Synthesis Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013045:Species Specificity
chemical_list: D010936:Plant Extracts; D011500:Protein Synthesis Inhibitors