Characterization of human interleukin-3 receptors on a multi-factor-dependent cell line.
Recombinant human interleukin-3 (hIL-3) was radioiodinated by Bolton-Hunter method with maintenance of biological activity. Using 125I-hIL-3, hIL-3 receptors were characterized on a multi-factor-dependent cell line TF-1. Equilibrium binding studies revealed the existence of a single class of binding sites (667 +/- 306 sites/cell) with a Kd of 173 +/- 25 pM. Affinity labeling of TF-1 cells with 125I-IL-3 yielded two bands of 150 kDa and 85 kDa, implying molecular weights of 135 kDa and 70 kDa for the hIL-3 receptors.
Kuwaki
T
T
Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Kitamura
T
T
Tojo
A
A
Matsuki
S
S
Tamai
Y
Y
Miyazono
K
K
Takaku
F
F
D002460:Cell Line; D003115:Colony-Stimulating Factors / Q000502:physiology*; D003432:Cross-Linking Reagents; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000502:physiology*; D006801:Humans; D007377:Interleukin-3 / Q000378:metabolism*; D007457:Iodine Radioisotopes; D007700:Kinetics; D008970:Molecular Weight; D011869:Radioligand Assay; D011971:Receptors, Immunologic / Q000032:analysis* / Q000302:isolation & purification; D016185:Receptors, Interleukin-3; D013696:Temperature
D003115:Colony-Stimulating Factors; D003432:Cross-Linking Reagents; D006133:Growth Substances; D007377:Interleukin-3; D007457:Iodine Radioisotopes; D011971:Receptors, Immunologic; D016185:Receptors, Interleukin-3; D016178:Granulocyte-Macrophage Colony-Stimulating Factor