Crystallization of thermitase, a thermostable subtilisin, from a sodium formate solution by means of an automated procedure.
A new crystal form of native thermitase has been obtained using sodium formate as the precipitating agent and employing an automated crystallization procedure. The crystals have the form of tetragonal bipyramids, the longest dimension being about 0.4 mm. The space group is P4(1)2(1)2 or P4(3)2(1)2, with a = 182 A and b = c = 53.3 A. The crystals diffract beyond 2.5 A.
Kelders
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Laboratory of Chemical Physics, University of Groningen, The Netherlands.
Kalk
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Hol
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D003460:Crystallization; D010450:Endopeptidases; D005561:Formates; D006358:Hot Temperature; D008722:Methods; D012697:Serine Endopeptidases
D005561:Formates; C030544:formic acid; D010450:Endopeptidases; D012697:Serine Endopeptidases; C022728:thermitase