Evidence for the presence of DNA primase in mitochondria of Saccharomyces cerevisiae.
Chromatography of a DNA polymerase preparation from mitochondria of Saccharomyces cerevisiae on DNA-cellulose column, using Tris-HCl (pH 7.5) buffer containing 0.6 M NaCl as eluent, was found to yield a fraction exhibiting DNA primase-like activity free of DNA polymerase. This fraction could support the synthesis of 12-15 residue-long oligoribonucleotides on single-stranded natural or synthetic DNA templates. The oligoribonucleotides could be further elongated by incorporation of deoxyribonucleotides in the presence of Klenow fragment.
Desai
S D
SD
Biochemistry Division, Bhabha Atomic Research Centre, Bombay, India.
Pasupathy
K
K
Chetty
K G
KG
Pradhan
D S
DS
D005591:Chemical Fractionation; D019915:DNA Primase; D004261:DNA Replication; D004271:DNA, Fungal / Q000302:isolation & purification / Q000378:metabolism* / Q000502:physiology; D004272:DNA, Mitochondrial / Q000302:isolation & purification / Q000378:metabolism* / Q000502:physiology; D004259:DNA-Directed DNA Polymerase / Q000302:isolation & purification / Q000502:physiology; D004591:Electrophoresis, Polyacrylamide Gel; D012316:RNA Nucleotidyltransferases / Q000302:isolation & purification / Q000378:metabolism* / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D012967:Sodium Dodecyl Sulfate; D013698:Templates, Genetic
D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D012967:Sodium Dodecyl Sulfate; D019915:DNA Primase; D012316:RNA Nucleotidyltransferases; D004259:DNA-Directed DNA Polymerase