Role of glycosylation in the incorporation of intrinsic mannoproteins into cell walls of Saccharomyces cerevisiae.
Cell wall mannoproteins from Saccharomyces cerevisiae are completely or partially incorporated into their final location when N-glycosylation is inhibited by tunicamycin. These include a 90-100 kDa species still containing O-linked oligomannose chains, derived from a N-glycosylated material larger than 120 kDa; and a 30.5 kDa peptide lacking mannose residues, derived from a 33 kDa species. For both species, the growth temperature influences the level of incorporation of the non N-glycosylated molecules. Secretion of the peptides lacking N-linked saccharide chains follows the route defined by sec mutants.
Sanz
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P
Departament de Microbiologia, Facultat de Farmacia, Universitat de Valencia, Spain.
Herrero
E
E
Sentandreu
R
R
D002473:Cell Wall / Q000378:metabolism; D006031:Glycosylation; D008562:Membrane Glycoproteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*; D014415:Tunicamycin / Q000494:pharmacology
D008562:Membrane Glycoproteins; C025363:mannoproteins; D014415:Tunicamycin