Detection of drug resistance in human ovarian carcinoma.
Drug-resistant cancer cells with the multidrug-resistance phenotype show overexpression of P-glycoprotein, and we therefore tested carcinoma tissue from five patients with stage III or IV ovarian cancer for P-glycoprotein using 265/F4 and C 219 monoclonal antibodies, prepared against membrane glycoproteins in colchicine-resistant CHO cells. Using immunofluorescence and immunoblotting techniques, one of the tumors showed a positive reaction. Using the pcDR 1.5 clone we found that the same cancer tissue had elevated expression of the genes responsible for multidrug resistance. The demonstration of elevated P-glycoprotein in ovarian carcinomas indicates that P-glycoprotein overexpression is not limited to experimental tumor models.
Volm
M
M
Department of Experimental Pathology, German Cancer Research Center, Heidelberg, FRG.
Efferth
T
T
Bak
M
M
Mattern
J
J
D002277:Carcinoma / Q000032:analysis* / Q000235:genetics; D004351:Drug Resistance / Q000235:genetics*; D005260:Female; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000032:analysis* / Q000235:genetics; D006801:Humans; D015151:Immunoblotting; D010051:Ovarian Neoplasms / Q000032:analysis* / Q000235:genetics
D006023:Glycoproteins