[The effect of depletion depletion macrophages on tumor-suppressive activity of polyactin A].
Trypan blue(T. blue) was used to block macrophages (M phi) for investigating the role of M phi in tumor-suppressing activity of polyactin A, a mannose-peptide immunomodulator extracted from alpha-streptococcus. Results showed that polyactin A could significantly inhibit the growth of transplanted S 180 tumor in mice. Anyhow, when M phi activities were blocked by T. blue, the protective effect of polyactin A was almost completely lost. Moreover, T. blue-treated mice showed larger tumor size than the controls. In addition, marked hemorrhagic necrosis was seen in the polyactin A-treated tumor, and the area of necrosis was larger than that in the controls. No significant difference was seen between T. blue-treated group and the controls. Furthermore, more M type ANAE+ cells were seen in the peripheral area of the tumors in polyactin A-treated groups than in any other group. All these findings suggest that the in vivo antitumor effect of polyactin A is produced mainly through M phi mechanisms.
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D000818:Animals; D005260:Female; D006020:Glycopeptides / Q000494:pharmacology*; D007155:Immunologic Factors / Q000494:pharmacology*; D008262:Macrophage Activation; D008264:Macrophages / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012510:Sarcoma 180 / Q000473:pathology*; D014343:Trypan Blue / Q000494:pharmacology
D006020:Glycopeptides; D007155:Immunologic Factors; C064233:polyactin A; D014343:Trypan Blue