title: From analysis to synthesis: new ligand binding sites on the lactate dehydrogenase framework. Part I.
abstract: In Part I of this article, the naturally evolved protein framework of lactate dehydrogenase is investigated by genetically introduced modifications which reveal the structural basis of its catalytic and substrate-binding properties. In Part II (to be published in the April issue of TIBS), this analytical information is exploited in the design of two modified forms of the enzyme; one which is specific for a new substrate and one which lacks allosteric regulation.
authors.forename: A R
authors.initials: AR
authors.lastname: Clarke
authors.forename: T
authors.initials: T
authors.lastname: Atkinson
authors.forename: J J
authors.initials: JJ
authors.lastname: Holbrook
mesh_terms: D001665:Binding Sites; D007770:L-Lactate Dehydrogenase / Q000096:biosynthesis* / Q000378:metabolism; D008024:Ligands / Q000378:metabolism*; D013329:Structure-Activity Relationship
chemical_list: D008024:Ligands; D007770:L-Lactate Dehydrogenase