Growth factor regulated expression of poly(A)+ binding protein messenger RNA.
A 72,000 mol wt protein designated PABP binds to the poly(A)+ track of messenger RNAs with high affinity and has been suggested to play an important role in mRNA metabolism in eucaryotic cells. We have employed a human PABP cDNA probe to study the expression of this gene at the mRNA level in BALB/c3T3 mouse cells under different growth conditions and in exponentially growing HeLa cells throughout the cell division cycle. We describe experiments which establish that in BALB/c3T3 cells the expression of this gene is growth factor regulated. Moreover, the gene behaves like a primary response gene in that its induction in quiescent cells does not require the prior synthesis of other growth factor-regulated proteins. In exponentially growing HeLa cells PABP mRNA is expressed throughout the cell division cycle indicating that the expression of this gene is not limited to a specific phase of the cell cycle.
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Department of Pathology, Temple University Medical School, Philadelphia, Pennsylvania 19140.
Jaskulski
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D000199:Actins / Q000235:genetics; D000818:Animals; D001769:Blood; D002352:Carrier Proteins / Q000235:genetics*; D002453:Cell Cycle; D002460:Cell Line; D003470:Culture Media; D003513:Cycloheximide / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects*; D006133:Growth Substances / Q000494:pharmacology*; D006367:HeLa Cells; D007328:Insulin / Q000494:pharmacology; D007399:Interphase; D008970:Molecular Weight; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D039102:Poly(A)-Binding Proteins; D012333:RNA, Messenger / Q000235:genetics*
D000199:Actins; D002352:Carrier Proteins; D003470:Culture Media; D006133:Growth Substances; D007328:Insulin; D010982:Platelet-Derived Growth Factor; D039102:Poly(A)-Binding Proteins; D012333:RNA, Messenger; D004815:Epidermal Growth Factor; D003513:Cycloheximide