Restriction endonucleases from microorganisms isolated in Brazil: an isoschizomer of HaeIII from a thermophilic Bacillus sp.
1. The isolation and characterization of a restriction endonuclease from a thermophilic strain of Bacillus is described. 2. The enzyme recognizes the palindromic sequence 5'...GGCC...3' as determined by PEI-cellulose chromatography of pancreatic DNAse and snake venom phosphodiesterase digestion products of labelled DNA fragments, analysis of restriction digests and direct sequence analysis. 3. The enzyme, denominated BspBR, is an isoschizomer of HaeIII and BspRI.
Cruz
A K
AK
Departamento de Bioquímica e Biologia Molecular, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brasil.
Pires
M Q
MQ
Lima
V G
VG
Morel
C M
CM
D001407:Bacillus / Q000201:enzymology*; D001483:Base Sequence; D001938:Brazil; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel
D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases