Effects of 2-deoxyglucose on Saccharomyces cerevisiae as observed by in vivo 31P-NMR.
Saccharomyces cerevisiae cells were treated with 2-deoxyglucose (1 mM) and the effects induced in the levels of phosphorus compounds and in the internal pH were monitored using 31P-NMR. Upon incubation with 2-deoxyglucose a strong decrease in the polyphosphate level was observed and the cytoplasmic pH decreased by about 0.4 units. This shows that 2-deoxyglucose strongly interferes with the cell conditions and consequently, the results of experiments in which 2-deoxyglucose was used to obtain deenergized cells should be carefully reanalysed.
Loureiro-Dias
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MC
Laboratory of Microbiology, Gulbenkian Institute of Science, Oeiras, Portugal.
Santos
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H
D003837:Deoxy Sugars / Q000494:pharmacology*; D003847:Deoxyglucose / Q000378:metabolism / Q000494:pharmacology*; D019298:Glucose-6-Phosphate / Q000031:analogs & derivatives*; D005958:Glucosephosphates / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D011122:Polyphosphates / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000378:metabolism
D003837:Deoxy Sugars; D005958:Glucosephosphates; D011122:Polyphosphates; C015785:2-deoxyglucose-6-phosphate; D019298:Glucose-6-Phosphate; D003847:Deoxyglucose