Studies on the myeloid synergistic factor from 5637: comparison with interleukin-1 alpha.
A synergistic factor that is produced by the human bladder carcinoma cell line 5637 (SF-1) stimulates primitive bone marrow progenitor cells, termed high proliferative-potential colony-forming cells (HPP-CFC), in the presence of an optimal dose of macrophage colony stimulating factor (CSF-1). Recent reports have demonstrated that interleukin-1 alpha (IL-1) is identical to hemopoietin 1 and have suggested that IL-1 is the synergistic factor present in 5637 conditioned medium (cm). We have compared the ability of recombinant human IL-1 alpha and partially purified preparations of SF-1 to synergize with optimal doses of CSF-1 to stimulate HPP-CFC. In all experiments performed the numbers of HPP-CFC colonies formed with IL-1 were significantly less than with SF-1. Replating experiments demonstrated that SF-1 plus CSF-1 generated HPP-CFC (responsive to IL-3 plus CSF-1); however, IL-1 plus CSF-1 resulted in no generation of HPP-CFC. Multiple factor combinations of IL-1 and SF-1 with G-CSF, GM-CSF, and CSF-1 also resulted in less HPP-CFC colony formation in cultures containing IL-1 compared with SF-1. Incubation of SF-1 with an antibody to IL-6 had no effect on HPP-CFC colony formation and IL-6 did not synergize with IL-1 plus CSF-1 or SF-1 plus CSF-1. These data suggest the presence of a factor in 5637 cm, which is distinct from G-CSF, GM-CSF, and IL-6, which synergizes with IL-1 to produce the SF-1 effect.
McNiece
I K
IK
Department of Internal Medicine, University of Virginia, Charlottesville.
Kriegler
A B
AB
Quesenberry
P J
PJ
D000818:Animals; D002277:Carcinoma; D003115:Colony-Stimulating Factors / Q000378:metabolism*; D005260:Female; D006133:Growth Substances / Q000378:metabolism*; D016298:Hematopoietic Cell Growth Factors; D007375:Interleukin-1 / Q000378:metabolism*; D016173:Macrophage Colony-Stimulating Factor; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000378:metabolism; D013234:Stem Cells / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000378:metabolism; D001749:Urinary Bladder Neoplasms
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D016298:Hematopoietic Cell Growth Factors; D007375:Interleukin-1; D011994:Recombinant Proteins; D016173:Macrophage Colony-Stimulating Factor