title: Purification and characterization of Saccharomyces cerevisiae transcription factor IIIA.
abstract: Saccharomyces cerevisiae Transcription Factor IIIA (TFIIIA) has been purified to apparent homogeneity. Two polypeptides copurified with TFIIIA activity. Yeast TFIIIA is a DNA-binding protein which exhibits a high affinity for the internal control region of the homologous 5 S ribosomal RNA gene. Characterization of the yeast protein indicates that it shares most, but not all, of the molecular properties of its Xenopus TFIIIA counterpart.
authors.affiliation: Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
authors.forename: C K
authors.initials: CK
authors.lastname: Wang
authors.forename: P A
authors.initials: PA
authors.lastname: Weil
mesh_terms: D000818:Animals; D001483:Base Sequence; D004275:DNA, Ribosomal / Q000235:genetics; D004268:DNA-Binding Proteins; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012335:RNA, Ribosomal / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D035582:Transcription Factor TFIIIA; D014157:Transcription Factors / Q000235:genetics / Q000302:isolation & purification*; D014981:Xenopus
chemical_list: D004275:DNA, Ribosomal; D004268:DNA-Binding Proteins; D012335:RNA, Ribosomal; D012341:RNA, Ribosomal, 5S; D035582:Transcription Factor TFIIIA; D014157:Transcription Factors