title: Lectin peroxidase conjugate reactivity in acquired cystic disease of the kidney.
abstract: The origins of acquired cysts, hyperplastic epithelia of cyst walls, and renal cell carcinomas were investigated by evaluating their lectin conjugate reactivity. Paraffin-embedded blocks from 9 patients with acquired cystic disease were examined by the high-sensitivity lectin-antilectin immune peroxidase method. 11-176 lesions in each patient, 690 lesions in total, were stained both with Tetragonolobus lotus lectin (T) and peanut lectin (P); the former is specific for proximal tubules and the latter for distal tubules and collecting ducts. Out of 606 acquired cysts with single-layered epithelia, 559 (92.2%) were positive for T and negative for P, and 66 out of 75 (88.0%) cysts with hyperplastic multilayered epithelia were positive for T and negative for P. Three out of 4 solid adenomas and, to varying degrees, 5 renal carcinomas revealed the same reaction. These results suggest that almost all cysts accompanying acquired cystic disease of the kidney, including those with single-layered and multilayered epithelia, as well as solid adenomas and renal cell carcinomas, are derived from proximal tubules.
authors.affiliation: Department of Internal Medicine, Kanazawa Medical University, Ishikawa, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Ishikawa
authors.forename: T
authors.initials: T
authors.lastname: Horiguchi
authors.forename: N
authors.initials: N
authors.lastname: Shikura
mesh_terms: D000328:Adult; D000368:Aged; D010367:Arachis; D005260:Female; D005643:Fucose / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D007668:Kidney / Q000378:metabolism*; D052177:Kidney Diseases, Cystic / Q000378:metabolism*; D007687:Kidney Tubules, Proximal / Q000378:metabolism; D037102:Lectins / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D019887:Peanut Agglutinin; D037121:Plant Lectins
chemical_list: D037102:Lectins; D019887:Peanut Agglutinin; D037121:Plant Lectins; C027112:fucose-binding lectin; D005643:Fucose