Assessment of the viability of haemopoietic cells in the livers of mouse fetuses stored at 4 degrees C. Comparison of various methods of testing their usefulness for haemopoietic transplantation.
The usefulness of three different tests available in experimental haematology for the assessment of the viability of fetal liver cells obtained from murine fetuses kept in refrigerator at 4 degrees C was compared. The usefulness of these cells as potential transplants for haemopoiesis reconstitution was assessed in the test with trypan blue, in the test based on clonal growth of GM-CFU in agar, and in the test of the splenic colony forming ability of CFU-S. Fetuses kept in refrigerator at 4 degrees C tested 16 hours after circulation arrest contained still about 70% of the initial number of the haemopoietic stem cells (CFU-S). The proportion of committed cells belonging to the granulocyto-monopoiesis line (GM-CFU) decreased at the same time to about 25% of the initial number, and was a sensitive indicator of hypoxia of the studied organ. The test for the viability of the cells based on the use of trypan blue gave results reflecting better the changes in the number of CFU-S than GM-CFU cells.
Ratajczak
M Z
MZ
Department of Immunology CSK WAM, Warszawa.
Szczylik
C
C
Berger
L
L
D000818:Animals; D016026:Bone Marrow Transplantation; D015925:Cryopreservation; D005333:Fetus / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D008099:Liver / Q000166:cytology* / Q000196:embryology; D051379:Mice; D013997:Time Factors; D014021:Tissue Preservation