Local tumor control following single dose irradiation of human melanoma xenografts: relationship to cellular radiosensitivity and influence of an immune response by the athymic mouse.
The potential usefulness of untreated congenitally athymic adult mice as hosts for human tumors in radiocurability studies was investigated using five human melanoma xenograft lines (E.E., E.F., G.E., M.F., V.N.). The tumor radiocurability was found to differ considerably among the lines; the radiation doses required to achieve local control of 50% of the tumors irradiated (TCD50 values) ranged from 29.6 +/- 2.1 (SE) to 67.9 +/- 3.5 Gy. Since the clinical relevance of experimentally determined TCD50 values depends on to what extent they are modified by a host immune response, a possible immune reactivity against the melanomas was investigated by comparing the radiocurability data with cell survival data measured in vitro after irradiation in vivo and by performing quantitative tumor transplantability studies. The radiocurability and the cell survival data were found to agree well for the E.F., G.E., and M.F. melanomas. Moreover, the number of tumor cells required to achieve tumors in 50% of the inoculation sites (TD50 values) in untreated and in whole-body irradiated mice were similar, suggesting that the TCD50 values measured for these lines were not significantly influenced by a host immune response. On the other hand, the E.E. and V.N. melanomas showed significantly lower TCD50 values in vivo than predicted theoretically from the in vitro cell survival data and a significantly lower number of tumor cells required to achieve tumors in 50% of the inoculation sites in whole-body irradiated than in untreated mice, suggesting that the radiocurability of these two lines was enhanced due to an immune response by the host. Athymic mice may thus express a significant immune reactivity against some human tumor xenograft lines but not against others. Consequently, TCD50 values measured for human tumors in athymic mice cannot be considered to be clinically relevant unless it has been verified that the tumors are not exposed to an immune reaction in the untreated host or the host has been rendered immunologically blank by immunosuppressive treatment.
Rofstad
E K
EK
Institute for Cancer Research, Norwegian Radium Hospital, Montebello, Oslo.
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis; D008545:Melanoma / Q000276:immunology / Q000473:pathology / Q000532:radiotherapy*; D051379:Mice; D008819:Mice, Nude; D011879:Radiotherapy Dosage; D014183:Transplantation, Heterologous; D014916:Whole-Body Irradiation