Extraction and rapid inactivation of proteins from Saccharomyces cerevisiae by trichloroacetic acid precipitation.
Methods currently used for the extraction of proteins from yeast involve relatively long time periods between sampling cells from a culture and analysis of their proteins by polyacrylamide gel electrophoresis-sodium dodecylsulphate. Often it is desirable to inactivate cellular metabolism rapidly after sampling and here we show that trichloroacetic acid precipitation techniques, often used for rapid extraction and inactivation of proteins from higher eukaryotes, can be adapted for use with organisms which have cell walls.
Wright
A P
AP
Centre for Biotechnology, Imperial College of Science and Technology, London, U.K.
Bruns
M
M
Hartley
B S
BS
D002473:Cell Wall / Q000378:metabolism; D011232:Chemical Precipitation; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000032:analysis / Q000302:isolation & purification*; D005898:Glass; D008863:Microspheres; D012441:Saccharomyces cerevisiae / Q000032:analysis* / Q000648:ultrastructure; D014238:Trichloroacetic Acid
D005656:Fungal Proteins; D014238:Trichloroacetic Acid