title: A fusion protein required for vesicle-mediated transport in both mammalian cells and yeast.
abstract: A protein sensitive to N-ethylmaleimide catalyses the fusion of transport vesicles with Golgi cisternae in a mammalian cell-free system. By cloning and sequencing its gene from Chinese hamster ovary cells and by use of in vitro assays, we show that this fusion protein is equivalent to the SEC18 gene product of the yeast Saccharomyces cerevisiae, known to be essential for vesicle-mediated transport from the endoplasmic reticulum to the Golgi apparatus. The mechanism of vesicular fusion is thus highly conserved, both between species and at different stages of transport.
authors.affiliation: Department of Biology, Princeton University, New Jersey 08540.
authors.forename: D W
authors.initials: DW
authors.lastname: Wilson
authors.forename: C A
authors.initials: CA
authors.lastname: Wilcox
authors.forename: G C
authors.initials: GC
authors.lastname: Flynn
authors.forename: E
authors.initials: E
authors.lastname: Chen
authors.forename: W J
authors.initials: WJ
authors.lastname: Kuang
authors.forename: W J
authors.initials: WJ
authors.lastname: Henzel
authors.forename: M R
authors.initials: MR
authors.lastname: Block
authors.forename: A
authors.initials: A
authors.lastname: Ullrich
authors.forename: J E
authors.initials: JE
authors.lastname: Rothman
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001692:Biological Transport; D002352:Carrier Proteins / Q000235:genetics* / Q000502:physiology; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D005033:Ethylmaleimide / Q000494:pharmacology*; D005796:Genes; D005800:Genes, Fungal; D006056:Golgi Apparatus / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D052066:N-Ethylmaleimide-Sensitive Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D033921:Vesicular Transport Proteins
chemical_list: D002352:Carrier Proteins; D033921:Vesicular Transport Proteins; D052066:N-Ethylmaleimide-Sensitive Proteins; D005033:Ethylmaleimide