title: The formation of eosinophil and neutrophil chemotactic activity during a pollen season and after allergen challenge.
abstract: Heat-stable (HS) and heat-labile (HL) neutrophil chemotactic activities (NCAs) have been demonstrated in serum after allergen challenge of subjects with asthma. In this investigation, we have studied the possible occurrence of similar activities in 20 atopic individuals on natural exposure to allergen, that is, during the birch-pollen season. Since eosinophil accumulation is a hallmark of an ongoing allergic inflammation in the respiratory tract also, the possible production of eosinophil chemotactic activity (ECA) was examined in serum after allergen challenge and at natural exposure to pollen. Both HL-NCA and HL-ECA were produced to a significant extent (p less than 0.001) during the season, with the peak of activities occurring simultaneously with the peak pollen count. HL-ECA was produced after allergen challenge of subjects with asthma in the laboratory, as has been demonstrated for NCA previously. The activity of the HS-NCA was unaltered during season. Gel-filtration studies of the major HL-NCA and HL-ECA indicated a molecular weight for both activities of 100 to 150,000, and the activities produced during season cochromatographed with the HL-NCA and HL-ECA produced after allergen challenge in the laboratory, suggesting that all these activities are due to one and the same molecule. The results suggest that the heat-labile chemotactic activity found in serum of atopic subjects and subjects with asthma after allergen exposure may be involved in the attraction of eosinophils and neutrophils to the site of allergic inflammation.
authors.affiliation: Department of Clinical Chemistry, University Hospital, Uppsala, Sweden.
authors.forename: L
authors.initials: L
authors.lastname: Håkansson
authors.forename: S
authors.initials: S
authors.lastname: Rak
authors.forename: R
authors.initials: R
authors.lastname: Dahl
authors.forename: P
authors.initials: P
authors.lastname: Venge
mesh_terms: D001249:Asthma / Q000276:immunology; D002630:Chemotactic Factors / Q000096:biosynthesis* / Q000097:blood; D002631:Chemotactic Factors, Eosinophil / Q000096:biosynthesis* / Q000097:blood; D002634:Chemotaxis, Leukocyte; D002850:Chromatography, Gel; D004804:Eosinophils / Q000502:physiology; D006801:Humans; D016209:Interleukin-8; D009504:Neutrophils / Q000502:physiology; D011058:Pollen / Q000276:immunology*
chemical_list: D002630:Chemotactic Factors; D002631:Chemotactic Factors, Eosinophil; D016209:Interleukin-8