[Catalytic component of calmodulin-independent adenylate cyclase from bovine brain. Isolation and determination of partial amino acid sequence].
The catalytic component of calmodulin-independent adenylate cyclase of cattle cerebral cortex was solubilized and purified to the homogeneous state. The conditions for preparative obtaining of the enzyme on the column with immobilized antibodies to adenylate cyclase were found. These antibodies were proved to interact with the calmodulin-independent rather than the calmodulin-dependent form of the enzyme. Molecular mass of the calmodulin-independent adenylate cyclase determined electrophoretically is 140 +/- 10 kDa. Amino acid composition of the enzyme and sequences of its fragments (in total 300 amino acid residues) obtained upon treatment with lysyl-specific proteinase from Achromobacter liticus were determined. Clone containing a cDNA 605 bp insertion coding for the 183 amino acid residue fragment of adenylate cyclase was isolated from the bovine brain cDNA library. Homology of this fragment to the known sequences of Escherichia coli and Bordetella pertussis adenylate cyclases was revealed.
Lipkin
V M
VM
Mirzoeva
S F
SF
Dranitsyna
S M
SM
Moshniakov
M V
MV
Petrov
V M
VM
D000262:Adenylyl Cyclases / Q000032:analysis / Q000302:isolation & purification*; D000421:Alcaligenes / Q000201:enzymology; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D001483:Base Sequence; D002384:Catalysis; D002417:Cattle; D002540:Cerebral Cortex / Q000201:enzymology*; D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D003001:Cloning, Molecular; D003488:Cyanogen Bromide; D004247:DNA / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D015151:Immunoblotting; D007163:Immunosorbent Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011233:Precipitin Tests; D012697:Serine Endopeptidases / Q000378:metabolism; D012995:Solubility
D000911:Antibodies, Monoclonal; D004247:DNA; D012697:Serine Endopeptidases; C036813:lysyl endopeptidase; D000262:Adenylyl Cyclases; D003488:Cyanogen Bromide
Kataliticheskiĭ komponent kal'modulinnezavisimoĭ adenilattsiklazy mozga byka. Vydelenie i opredelenie chastichnoĭ aminokislotnoĭ posledovatel'nosti.