title: The yeast pyruvate kinase gene does not contain a string of non-preferred codons: revised nucleotide sequence.
abstract: The sequence of the gene encoding pyruvate kinase from Saccharomyces cerevisiae was re-determined because of failures with oligonucleotide-directed mutagenesis experiments involving a region thought to contain a string of five contiguous non-preferred codons. This region was found to be difficult to sequence and was shown to have three extra bases when compared with the published sequence [(1983) J. Biol. Chem. 258, 2193-2201]. The revised sequence demonstrates that the yeast pyruvate kinase gene does not have a cluster of non-preferred codons, and that it therefore is not an example of the class of genes which possibly exhibit translational control by the presence of non-preferred codons.
authors.affiliation: Department of Biochemistry, University of Edinburgh, Scotland.
authors.forename: T
authors.initials: T
authors.lastname: McNally
authors.forename: I J
authors.initials: IJ
authors.lastname: Purvis
authors.forename: L A
authors.initials: LA
authors.lastname: Fothergill-Gilmore
authors.forename: A J
authors.initials: AJ
authors.lastname: Brown
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003062:Codon; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D011770:Pyruvate Kinase / Q000235:genetics*; D012333:RNA, Messenger; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics
chemical_list: D003062:Codon; D012333:RNA, Messenger; D011770:Pyruvate Kinase