The complete islet amyloid polypeptide precursor is encoded by two exons.
Islet amyloid polypeptide (IAPP) is the 37-amino acid peptide subunit of amyloid found in pancreatic islets of type 2 diabetic patients and in insulinomas. Recently, we isolated the human gene encoding IAPP [(1988) FEBS Lett. 239, 227-232]. We now report the nucleotide sequences of a human insulinoma cDNA encoding a complete IAPP precursor, and of the corresponding parts of the IAPP gene. Two exons, which are approx. 5 kb apart in the human genome, encode the 89-amino acid pre-pro-IAPP. At least one additional exon is present further upstream in the IAPP gene. A putative signal sequence at the amino-terminus of the precursor suggests that IAPP is a secreted protein.
Mosselman
S
S
Institute of Molecular Biology and Medical Biotechnology, Utrecht, The Netherlands.
Höppener
J W
JW
Lips
C J
CJ
Jansz
H S
HS
D000595:Amino Acid Sequence; D000682:Amyloid / Q000235:genetics*; D001483:Base Sequence; D002116:Calcitonin / Q000235:genetics; D015740:Calcitonin Gene-Related Peptide; D003062:Codon; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D005091:Exons; D006801:Humans; D007340:Insulinoma / Q000032:analysis; D058228:Islet Amyloid Polypeptide; D007515:Islets of Langerhans / Q000032:analysis*; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010190:Pancreatic Neoplasms / Q000032:analysis; D011498:Protein Precursors / Q000235:genetics*; D021382:Protein Sorting Signals / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D000682:Amyloid; D003062:Codon; D015342:DNA Probes; D058228:Islet Amyloid Polypeptide; D009479:Neuropeptides; D011498:Protein Precursors; D021382:Protein Sorting Signals; D002116:Calcitonin; D004247:DNA; D015740:Calcitonin Gene-Related Peptide