title: A double buffy coat method for red cell removal from ABO-incompatible marrow.
abstract: Hemolytic reactions caused by transfusion of ABO-incompatible marrow can be ameliorated by either reduction of isohemagglutinins in the recipient or depletion of incompatible red cells from the harvested marrow. This article describes a rapid and reliable method for removal of incompatible marrow red cells on a blood cell processor using a double buffy coat technique. In five allogeneic bone marrow transplants, the maximum value of transfused incompatible red cells was 8.8 ml. There was no evidence of a hemolytic transfusion reaction in any patient. The median cell recovery for nucleated marrow cells and progenitor cells was 77 and 104 percent, respectively. Engraftment occurred at a median of 13 days (range, 11-21 days) after transplantation. The double buffy coat method of red cell depletion is an acceptable method for processing ABO-incompatible marrow.
authors.affiliation: Department of Medicine, Montefiore Hospital, Pittsburgh, Pennsylvania.
authors.forename: C S
authors.initials: CS
authors.lastname: Rosenfeld
authors.forename: H
authors.initials: H
authors.lastname: Tedrow
authors.forename: F
authors.initials: F
authors.lastname: Boegel
authors.forename: C
authors.initials: C
authors.lastname: Gremba
authors.forename: R K
authors.initials: RK
authors.lastname: Shadduck
mesh_terms: D000017:ABO Blood-Group System / Q000276:immunology*; D000743:Anemia, Hemolytic / Q000209:etiology; D001787:Blood Group Incompatibility / Q000097:blood* / Q000209:etiology; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D002452:Cell Count; D002469:Cell Separation / Q000379:methods; D017707:Erythrocyte Transfusion; D004911:Erythrocyte Volume; D004912:Erythrocytes; D006085:Graft Survival; D006801:Humans; D017708:Leukocyte Transfusion; D007962:Leukocytes; D065227:Transfusion Reaction
chemical_list: D000017:ABO Blood-Group System