title: [Bioluminescence assay of luteinizing hormone in plasma and urine].
abstract: The authors describe a bioluminescent immunoassay of LH in plasma and urine. It uses two monoclonal antibodies, one is labelled with glucose-6-phosphate-dehydrogenase, the other one is coimmobilized together with bioluminescent enzymes from marine bacteria on the same adsorbent (Sepharose). This assay can be performed directly on 20 microliters plasma or 10 microliters urine. The protocol is very fast, no separation step is required to remove the excess labeled antibodies. The inhibitory effect of the biological sample on luminescent reaction is determined by adding NADH to the assay tubes. The working range of this assay is 3 to 300 Ul/l, with a sensitivity of detection of 0.5 Ul/l. Recovery, linearity, within and between assay precision were evaluated and appeared to be satisfactory. The authors have observed a good correlation between results obtained with our method and with radioimmunoassay.
authors.affiliation: INSERM U58 60 Montpellier.
authors.forename: B
authors.initials: B
authors.lastname: Terouanne
authors.forename: S
authors.initials: S
authors.lastname: Alameddine
authors.forename: J L
authors.initials: JL
authors.lastname: Martin
authors.forename: J C
authors.initials: JC
authors.lastname: Nicolas
authors.forename: P
authors.initials: P
authors.lastname: Cristol
authors.forename: C
authors.initials: C
authors.lastname: Sultan
authors.forename: A
authors.initials: A
authors.lastname: Crastes de Paulet
mesh_terms: D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008163:Luminescent Measurements; D008164:Luminescent Proteins; D007986:Luteinizing Hormone / Q000032:analysis* / Q000097:blood / Q000652:urine
chemical_list: D008164:Luminescent Proteins; D007986:Luteinizing Hormone
vernacular_title: Dosage par bioluminescence de l'hormone lutéinisante dans le plasma et l'urine.