Cardiorespiratory effects of DL-homocysteic acid in caudal ventrolateral medulla.
We carried out experiments in urethan-anesthetized rats to determine 1) whether increasing the activity of small groups of neurons in the caudal ventrolateral medulla (CVLM) by injecting picomoles of an excitatory amino acid altered cardiovascular and/or respiratory homeostasis and 2) whether the depressor responses after chemical excitation in the CVLM were elicited only from the immunohistochemically identified catecholamine-containing cell group. In discrete sites in the CVLM, unilateral injections of 1-12 nl (20-240 pmol) of DL-homocysteic acid (DLH; 20 mM, pH 7.4) selectively or concomitantly inhibited arterial pressure, heart rate, and diaphragm electromyogram (EMG) activity. In the region in which chemical excitation slowed breathing, units were recorded extracellularly that discharged with respiratory periodicity. Sites where the smallest volumes of DLH decreased arterial pressure were located outside the immunohistochemically identified DBH-positive cell bodies. These data suggest that either the same or neighboring neurons in the CVLM are involved in the central neural circuitry for both cardiovascular and respiratory control and that cells other than the catecholaminergic cell group are important in medullary depressor responses.
Bonham
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AC
Department of Physiology, Northwestern University Medical School, Chicago, Illinois 60611.
Jeske
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D000818:Animals; D001794:Blood Pressure / Q000187:drug effects*; D004299:Dopamine beta-Hydroxylase / Q000378:metabolism; D006339:Heart Rate / Q000187:drug effects*; D006651:Histocytochemistry; D006710:Homocysteine / Q000031:analogs & derivatives* / Q000494:pharmacology; D008297:Male; D008526:Medulla Oblongata / Q000187:drug effects / Q000201:enzymology / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D012119:Respiration / Q000187:drug effects*; D013238:Stereotaxic Techniques
D006710:Homocysteine; C007956:homocysteic acid; D004299:Dopamine beta-Hydroxylase