Demonstration of anti-bradykinin compounds in callus cultures of Mandevilla velutina.
Calluses from stems and leaves of Mandevilla velutina were grown in culture for 30, 45 and 60 days. Thin-layer chromatography of ethyl acetate extracts of calluses from stems of M. velutina revealed the presence of 6 compounds. Two of them had RF values identical to those of the anti-bradykinin (BK) compounds MV8609 and MV8610 previously isolated from the natural plant. The ethyl acetate extract (20 to 80 micrograms/ml) from stem callus culture antagonized in a concentration-dependent and reversible manner contractions caused by BK (0.1-1000 nM) in the isolated rat uterus. The extracts obtained from calluses cultured for 30, 45 and 60 days were about 79-, 63- and 31-fold more potent, respectively, in antagonizing BK than the crude extract obtained from the rhizome of the plant.
Calixto
J B
JB
Departamentos de Farmacologia, Universidade Federal de Santa Catarina, Brasil.
Silva
A L
AL
Reis
M S
MS
Costa
R M
RM
Yunes
J A
JA
Cruz
A B
AB
Yunes
R A
RA
D000818:Animals; D001920:Bradykinin / Q000037:antagonists & inhibitors*; D001938:Brazil; D002855:Chromatography, Thin Layer; D003470:Culture Media; D005260:Female; D010936:Plant Extracts / Q000032:analysis* / Q000494:pharmacology; D010946:Plants, Medicinal; D051381:Rats; D014590:Uterine Contraction / Q000187:drug effects
D003470:Culture Media; D010936:Plant Extracts; D001920:Bradykinin