title: A new Saccharomyces cerevisiae mnn mutant N-linked oligosaccharide structure.
abstract: We find that the N-linked Man8GlcNAc2- core oligosaccharide of Saccharomyces cerevisiae mnn mutant mannoproteins is enlarged by the addition of the outer chain to the alpha 1----3-linked mannose in the side chain that is attached to the beta 1----4-linked mannose rather than by addition to the terminal alpha 1----6-linked mannose. This conclusion is derived from structural studies on a phosphorylated oligosaccharide fraction and from mass spectral fragment analysis of neutral core oligosaccharides.
authors.affiliation: Department of Biochemistry, University of California, Berkeley 94720.
authors.forename: L M
authors.initials: LM
authors.lastname: Hernández
authors.forename: L
authors.initials: L
authors.lastname: Ballou
authors.forename: E
authors.initials: E
authors.lastname: Alvarado
authors.forename: B L
authors.initials: BL
authors.lastname: Gillece-Castro
authors.forename: A L
authors.initials: AL
authors.lastname: Burlingame
authors.forename: C E
authors.initials: CE
authors.lastname: Ballou
mesh_terms: D002236:Carbohydrate Conformation; D015180:Electrophoresis, Gel, Two-Dimensional; D009682:Magnetic Resonance Spectroscopy; D009154:Mutation; D009844:Oligosaccharides; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*
chemical_list: D009844:Oligosaccharides