The saga of IMAC and MIT.
Immobilized Metal-ion Affinity Chromatography, IMAC, has been gaining in popularity as the purification technique of choice for proteins and peptides. IMAC of proteins on transition metals (Co, Ni, Cu, Zn) can be rationalized in terms of the coordination of histidine residues. Brief accounts of the principles of IMAC, its anticipated development and plausible applications are presented. Metal Ion Transfer, MIT, may offer an efficient means to deplete a metal ion from a metalloprotein or, conversely, to charge its apo form with a metal.
Sulkowski
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D002846:Chromatography, Affinity; D006639:Histidine; D008670:Metals; D011485:Protein Binding; D011506:Proteins / Q000302:isolation & purification*
D008670:Metals; D011506:Proteins; D006639:Histidine