title: Construction of an ordered cosmid collection of the Escherichia coli K-12 W3110 chromosome.
abstract: A cosmid library of the Escherichia coli K-12 W3110 chromosome was constructed in which clones were assigned to locations on the chromosome map by hybridization and genetic marker complementation tests. Approximately 70% of the genome was represented by this library. The identified clones can be maintained in the homologous system and would facilitate genetic studies of E. coli.
authors.affiliation: Department of Biology, Faculty of Science, Nagoya University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Tabata
authors.forename: A
authors.initials: A
authors.lastname: Higashitani
authors.forename: M
authors.initials: M
authors.lastname: Takanami
authors.forename: K
authors.initials: K
authors.lastname: Akiyama
authors.forename: Y
authors.initials: Y
authors.lastname: Kohara
authors.forename: Y
authors.initials: Y
authors.lastname: Nishimura
authors.forename: A
authors.initials: A
authors.lastname: Nishimura
authors.forename: S
authors.initials: S
authors.lastname: Yasuda
authors.forename: Y
authors.initials: Y
authors.lastname: Hirota
mesh_terms: D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D003360:Cosmids; D004926:Escherichia coli / Q000235:genetics*; D005838:Genotype