Identification of a novel gene encoding an insulin-responsive glucose transporter protein.
Insulin, rapidly and independently of new protein synthesis, stimulates glucose transport in sensitive target tissues. A cDNA has been cloned from a skeletal muscle library that encodes a novel glucose transporter protein exhibiting the following properties of an insulin-regulated hexose carrier protein: it is expressed exclusively in adipose tissue, skeletal muscle and heart, the principal organs with insulin-responsive glucose transport; RNA transcribed from the muscle cDNA, when expressed in Xenopus oocytes, encodes a protein capable of cytochalasin B inhibitable 2-deoxyglucose transport; and treatment of isolated rat adipocytes with insulin effects a redistribution of "muscle" transports from low density microsomes to the plasma membrane to an extent comparable to the activation of glucose transport.
Birnbaum
M J
MJ
Department of Cellular and Molecular Physiology, Harvard Medical School, Boston, Massachusetts 02115.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005796:Genes; D007328:Insulin / Q000235:genetics / Q000502:physiology*; D009004:Monosaccharide Transport Proteins / Q000235:genetics*; D009132:Muscles / Q000032:analysis; D014176:Protein Biosynthesis; D012333:RNA, Messenger; D051381:Rats
D007328:Insulin; D009004:Monosaccharide Transport Proteins; D012333:RNA, Messenger