[Effect of cigarette smoke condensate on unscheduled DNA synthesis (UDS) in human lung fibroblast cells].
The effect of cigarette smoke condensate (CSC) on UDS in human lung fibroblast cells (2BS) was studied. The results showed that CSC induced a marked increase in UDS of 2BS cells in a dose-dependent manner. 3H/14C ratio was 1.77 +/- 0.34 at a dose of 125 micrograms/ml of CSC as compared with 1.33 +/- 0.04 of the control. Catalase, at doses of 40, 60 units/ml, could significantly inhibit UDS induced by CSC (75 micrograms/ml), but after inactivation catalase had no effect. It is suggested that the increase in UDS is due to free radicals generated from CSC.
Gu
M
M
Cancer Institute, Chinese Academy of Medical Sciences, Beijing.
D002374:Catalase / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D005333:Fetus; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D008168:Lung / Q000166:cytology / Q000378:metabolism*; D010947:Plants, Toxic; D012906:Smoke / Q000009:adverse effects*; D014026:Nicotiana
D012906:Smoke; D004247:DNA; D002374:Catalase