title: Lipopolysaccharide interaction with lysozyme. Binding of lipopolysaccharide to lysozyme and inhibition of lysozyme enzymatic activity.
abstract: Experiments have been carried out to characterize the binding of lysozyme (LZM) to bacteriol lipopolysaccharide (LPS). The formation of LPS.LZM complexes can be readily demonstrated using either physical-chemical separation techniques or a radiolabeled photoaffinity LPS probe. The binding affinity of LZM for LPS has been estimated to be approximately 10(8) liters/mol. Binding of LPS results in loss of LZM enzymatic activity by a noncompetitive inhibition, as assessed by either particulate or soluble substrates. This interaction of LPS with LZM is dictated primarily by hydrophobic interactions and appears to be a general property of both constituents. Binding can be demonstrated with LZM of both human and avian sources, as well as with LPS isolated from a variety of Gram-negative organisms. The addition of LPS to biologically relevant fluids containing LZM results in dose-dependent inhibition of LZM enzymatic activity suggesting that such interactions may have relevance in Gram-negative infections. Finally LZM has been shown to reduce the endotoxic activity of LPS as assessed by gelation of Limulus amoebocyte lysates.
authors.affiliation: Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City 66103.
authors.forename: N
authors.initials: N
authors.lastname: Ohno
authors.forename: D C
authors.initials: DC
authors.lastname: Morrison
mesh_terms: D000345:Affinity Labels; D000818:Animals; D001665:Binding Sites; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002645:Chickens; D002850:Chromatography, Gel; D004229:Dithiothreitol; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D006801:Humans; D008070:Lipopolysaccharides / Q000378:metabolism*; D008837:Micrococcus / Q000201:enzymology; D008895:Milk, Human / Q000201:enzymology; D008970:Molecular Weight; D009113:Muramidase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010777:Photochemistry; D014422:Turkeys
chemical_list: D000345:Affinity Labels; D008070:Lipopolysaccharides; D009113:Muramidase; D004229:Dithiothreitol