title: Partial purification and characterization of a new p36/40 tyrosine protein kinase from HL-60.
abstract: A major peak of tyrosine protein kinase activity was partially purified from a Triton X100 extract of HL-60. This preparation submitted to high pressure gel filtration was eluted at a volume corresponding to a mass of 35/40 kD. This activity was insensitive to EGF and insulin. Autoradiographs of the preparations incubated with [gamma P32]-ATP and separated by electrophoresis do not give any evidence that autophosphorylation occurs for that particular tyrosine protein kinase. Furthermore, we failed to immunoprecipitate the enzyme with a specific antiphosphotyrosine antibody and anti v-src antibody. All the data presented herein suggest that this enzyme has not been previously purified.
authors.affiliation: Division Pathologies Cancereuses et Immunes, Institut de Recherches SERVIER, Suresnes, France.
authors.forename: J A
authors.initials: JA
authors.lastname: Boutin
authors.forename: A P
authors.initials: AP
authors.lastname: Ernould
authors.forename: A
authors.initials: A
authors.lastname: Genton
authors.forename: C A
authors.initials: CA
authors.lastname: Cudennec
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001345:Autoradiography; D001667:Binding, Competitive; D002851:Chromatography, High Pressure Liquid; D004587:Electrophoresis, Agar Gel; D004815:Epidermal Growth Factor / Q000494:pharmacology; D006160:Guanosine Triphosphate / Q000378:metabolism; D006801:Humans; D007163:Immunosorbent Techniques; D007328:Insulin / Q000494:pharmacology; D015473:Leukemia, Promyelocytic, Acute / Q000201:enzymology*; D008970:Molecular Weight; D017830:Octoxynol; D010766:Phosphorylation; D011092:Polyethylene Glycols; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000378:metabolism; D013379:Substrate Specificity; D014407:Tumor Cells, Cultured
chemical_list: D007328:Insulin; D011092:Polyethylene Glycols; D004815:Epidermal Growth Factor; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D017830:Octoxynol; D011505:Protein-Tyrosine Kinases