Nucleotide sequence of a citrate utilization gene from Citrobacter amalonaticus.
The chromosomal DNA fragment (BamHI-BglII fragment of 2,972 base pairs) conferring citrate utilization in Citrobacter amalonaticus ATCC 25405 was cloned and sequenced. Two genes (citA and citB) identified in the Cit+ determinant were found in a BamHI-BglII fragment from C. amalonaticus. Southern DNA-DNA hybridization experiments and the construction of Cit- mutants of C. amalonaticus showed that C. amalonaticus has a single copy of the cit gene on the chromosome.
Daimon
H
H
Department of Veterinary Public Health, Obihiro University of Agriculture and Veterinary Medicine, Japan.
Ishiguro
N
N
Shinagawa
M
M
Sato
G
G
D001483:Base Sequence; D015139:Blotting, Southern; D002951:Citrates / Q000378:metabolism*; D002954:Citrobacter / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009154:Mutation; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping
D002951:Citrates