Prolonged in vitro exposure of Syrian hamster embryo cells to 3-aminobenzamide induces transformation and chromosomal alterations but not gene mutations.
To investigate the genetic mechanisms involved in morphological transformation of Syrian hamster embryo (SHE) cells, we have studied the transforming potential of 3-aminobenzamide (3-AB). It was found that prolonged exposure to 3-AB induced morphological transformation of SHE cells as well as C3H10T 1/2 cells. At similar doses, 3-AB induced SCEs and chromosomal alterations (gaps, breaks and exchanges) in SHE cells, but no mutations at the hypoxanthine-guanine phosphoribosyl transferase locus. These data strongly suggest morphological transformation can result from genetic alterations other than gene mutations. The possible nature of these genetic alterations involved will be discussed.
De Kok
A J
AJ
Department of Radiation Genetics, Sylvius Laboratories, State University of Leiden, The Netherlands.
Sips
H
H
Den Engelse
L
L
Simons
J W
JW
D000818:Animals; D001549:Benzamides / Q000494:pharmacology*; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D009154:Mutation; D012854:Sister Chromatid Exchange
D001549:Benzamides; C025160:3-aminobenzamide