Production of H-Y antibody in the ascites fluid of mouse and localization of the antigen on cells and tissues.
A procedure is described for the production of large amounts of ascites fluid containing specific H-Y antibody. The distribution of H-Y antigen on mouse epididymal spermatozoa, thymocytes, and splenocytes was carried out using this specific antibody in the microcytotoxicity test and ELISA. Employing the indirect immunofluorescent technique, the H-Y antigen was localized on the acrosomal membrane of mouse epididymal and washed ejaculated human spermatozoa and on the entire membrane of mouse splenocytes and thymocytes. Immunohistochemical localization of the antigen in the testicular section indicated its presence in the cytoplasm of Leydig cells and on the membrane of Sertoli cells and sperm heads.
Iyer
S V
SV
Hybridoma Section, Institute for Research in Reproduction, Bombay, India.
Nandedkar
T D
TD
Hegde
U C
UC
D000177:Acrosome / Q000276:immunology; D000818:Animals; D001202:Ascitic Fluid / Q000276:immunology; D004822:Epididymis / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006182:H-Y Antigen / Q000302:isolation & purification*; D006801:Humans; D007150:Immunohistochemistry; D007518:Isoantibodies / Q000096:biosynthesis*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D013094:Spermatozoa / Q000276:immunology; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D006182:H-Y Antigen; C051256:H-Y antibody; D007518:Isoantibodies