In vitro effects of ethanol and acetaldehyde on lymphoid cells as determined by 31P NMR spectroscopy.
We have used NMR spectroscopy to test the direct in vitro effects of ethanol and its metabolite acetaldehyde on a human leukemic T cell line (Molt-4) and normal murine spleen cells. The metabolic status of phosphate metabolites (phosphomonoesters including sugar phosphate, inorganic phosphate and ATP's) in cell suspension was monitored by 31P NMR spectroscopy. Spectral changes were observed in the intensity of inorganic phosphate and ATP. Addition of high concentrations of ethanol (400-1600 mM) to Molt-4 cells resulted in very little spectral change. However, the addition of 40 microM acetaldehyde resulted in substantially increased inorganic phosphate (Pi) signals, and decreased phosphomonoesters and ATP signals. Similar changes, but to a lesser degree, were observed with normal mouse spleen cells.
Walia
A S
AS
Birmingham Veterans Administration Hospital, University of Alabama.
Lamon
E W
EW
D000079:Acetaldehyde / Q000494:pharmacology*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008808:Mice, Inbred CBA; D010758:Phosphorus; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
D010758:Phosphorus; D000431:Ethanol; D000255:Adenosine Triphosphate; D000079:Acetaldehyde