[Enzyme immunoassay for arginine vasopressin (AVP). 2. Serum interference and the measurement of plasma samples].
The effect of serum interference on enzyme immunoassay (EIA) for arginine vasopressin (AVP) was studied. Before measurement, plasma was applied on an affinity chromatography to remove AVP and then treated with the acetone-ether method which is usually used for AVP extraction of plasma samples. The AVP-free plasma extract was used to obtain a plasma AVP standard which was compared with various buffer standards of AVP. As a result, the enzyme activities of plasma AVP standard were always lower than those of buffer standards even though the concentration of buffer was increased or added by gelatin. Therefore, EIA for AVP was clinically applicable by using the AVP-free plasma for the standard. Plasma samples were drawn in various clinical conditions and measured for AVP by plasma AVP standard to examine reproducibility and correlation with radioimmunoassay (RIA). The values measured repetitively for the same samples were reproducible. The values measured by EIA correlated well with those measured by RIA (y = 0.67 + 1.13x, r = 0.96). These indicates that EIA for AVP could be applied clinically to measure physiological levels of plasma AVP.
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D001127:Arginine Vasopressin / Q000097:blood*; D002846:Chromatography, Affinity; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male
D001127:Arginine Vasopressin