Endothelin stimulates aldosterone biosynthesis by dispersed rabbit adreno-capsular cells.
The effect of endothelin on aldosterone production by dispersed adreno-capsular cells from rabbits was examined. Porcine endothelin stimulated aldosterone production dose-dependently with an EC50 of 5 x 10(-14) M, but had no effect on corticosterone production. A calcium channel blocker, nicardipine, completely inhibited the stimulatory effect of endothelin on aldosterone production. Endothelin induced prompt and sustained increase in intracellular Ca2+ in fura-2-loaded cells, and nicardipine inhibited this increase in intracellular Ca2+. These results indicate that endothelin stimulates aldosterone biosynthesis in dispersed zona glomerulosa cells of rabbits, and that its effects is related to increase in intracellular calcium through voltage-dependent calcium channels.
Morishita
R
R
Department of Geriatric Medicine, Osaka University Medical School, Japan.
Higaki
J
J
Ogihara
T
T
D000450:Aldosterone / Q000096:biosynthesis*; D000818:Animals; D002118:Calcium / Q000096:biosynthesis; D002469:Cell Separation; D003345:Corticosterone / Q000096:biosynthesis; D016232:Endothelins; D004730:Endothelium, Vascular / Q000502:physiology*; D008297:Male; D009529:Nicardipine / Q000494:pharmacology; D010455:Peptides / Q000502:physiology*; D011817:Rabbits; D015384:Zona Glomerulosa / Q000166:cytology / Q000502:physiology*
D016232:Endothelins; D010455:Peptides; D000450:Aldosterone; D009529:Nicardipine; D002118:Calcium; D003345:Corticosterone