A human airway smooth muscle cell line that retains physiological responsiveness.
We report the development of a nontransformed line of human airway smooth muscle cells retaining smooth muscle-specific contractile protein expression and physiological responsiveness to agonists implicated in inflammatory airway diseases. Specific responses to histamine, leukotrienes, bradykinin, platelet-activating factor, substance P, and thromboxane analogues are demonstrated as well as functional coupling to beta-adrenergic receptors. The cell line was characterized using indirect immunofluorescence, as well as electrophoretic separation and immunoblot analysis of smooth muscle-specific actin. Functional responses were assessed by measurements of cytosolic calcium and stimulation of adenosine 3',5'-cyclic monophosphate production. The cells retain their responsiveness over many population doublings and should be a useful model to examine specific receptor-effector mechanisms, as well as the effects of neurohumoral agents on the regulation of airway smooth muscle growth and differentiation.
Panettieri
R A
RA
Department of Medicine, University of Pennsylvania, Philadelphia 19104-6046.
Murray
R K
RK
DePalo
L R
LR
Yadvish
P A
PA
Kotlikoff
M I
MI
D000199:Actins / Q000032:analysis; D000205:Actomyosin / Q000032:analysis; D002118:Calcium / Q000378:metabolism; D002455:Cell Division; D002460:Cell Line; D046508:Culture Techniques / Q000379:methods; D003600:Cytosol / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D007700:Kinetics; D008970:Molecular Weight; D009130:Muscle, Smooth / Q000502:physiology*; D014132:Trachea / Q000502:physiology
D000199:Actins; D000205:Actomyosin; D002118:Calcium