Location of a synergistic factor in the capsule of a granulosis virus of the armyworm, Pseudaletia unipuncta.
A synergistic factor (SyF), which enhanced the infection of nuclear polyhedrosis viruses, was purified from capsules of a Pseudaletia unipuncta granulosis virus (Hawaiian strain) by immune affinity chromatography. The isolated SyF consisted primarily of a protein with molecular mass 98 kDa. The recovery rate depended on the alkali used to dissolve the capsules: the highest rate occurred with 0.05 M Na2CO3-0.05 M NaCl, followed in turn with 0.02-0.05 M NaOH and 0.04 M NaOH-0.05 M glycine. The solubilized components from untreated capsules contained 98- and 100-kDa proteins in addition to the matrix protein (29 kDa) and its decomposed products, while those from heat-treated capsules contained only the 100-kDa protein. Virons liberated from the capsules with the glycine buffer contained three proteins (33, 98, and 100 kDa) serologically related to the SyF. Immunoelectron microscopy of infected tissue and purified virions revealed the localization of the SyF antigens on the viral envelope.
Zhu
Y
Y
Hukuhara
T
T
Tamura
K
K
D000818:Animals; D001685:Biological Factors / Q000302:isolation & purification* / Q000502:physiology; D002213:Capsid / Q000032:analysis; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D007304:Insect Viruses / Q000032:analysis* / Q000472:pathogenicity; D007814:Larva / Q000382:microbiology; D009036:Moths / Q000382:microbiology; D014758:Viral Core Proteins / Q000032:analysis; D014759:Viral Envelope Proteins / Q000032:analysis*
D001685:Biological Factors; D014758:Viral Core Proteins; D014759:Viral Envelope Proteins