A synthetic intron in a naturally intronless yeast pre-tRNA is spliced efficiently in vivo.
Saccharomyces cerevisiae glutamine tRNA(CAG) is encoded by an intronless, single-copy gene, SUP60. We have imposed a requirement for splicing in the biosynthesis of this tRNA by inserting a synthetic intron in the SUP60 gene. Genetic analysis demonstrated that the interrupted gene produces a functional, mature tRNA product in vivo.
Winey
M
M
Laboratory of Genetics, University of Wisconsin, Madison 53706.
Edelman
I
I
Culbertson
M R
MR
D005813:Genes, Synthetic; D007438:Introns; D009154:Mutation; D012322:RNA Precursors / Q000378:metabolism*; D012326:RNA Splicing; D012343:RNA, Transfer / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
D012322:RNA Precursors; D012343:RNA, Transfer