Abnormalities in the NC1 domain of collagen type IV in GBM in canine hereditary nephritis.
Samoyed hereditary glomerulopathy (SHG) in dogs serves as a model for human X-linked hereditary nephritis (HN). We previously showed that glomerular capillaries of affected males did not stain by immunofluorescence (IF) using serum from a patient with Goodpasture's syndrome. Our goal in the present study was to determine whether the NC1 domain of the collagen type IV molecule, which contains Goodpasture antigen (GPA), could be demonstrated in these dogs, and to assess its immunological reactivity. By SDS-PAGE, NC1 in collagenase digests of glomerular basement membranes (GBM) of unaffected and carrier female dogs in the family with SHG showed 24 kilodalton (kD), 26 kD and 28 kD monomer, and 46 kD and 47 kD dimer components, but the 24 kD monomer was diminished in the affected males. By IF, a rabbit antibody to NCl stained glomerular capillaries of unaffected, affected male, and carrier female dogs. In contrast, a human anti-GBM plasmapheresis fluid (PPF) stained glomerular capillaries of only the unaffected and carrier female dogs. By RIA, both antibodies reacted strongly with NCl in collagenase digests of GBM of the unaffected and carrier female dogs, but showed reduced reactivity with NCl of affected males. By Western blotting, both antibodies bound to dimers and 24 kD and 26 kD monomers of the NCl domain in collagenase digests of GBM of unaffected and carrier female dogs. However, in affected males, the rabbit anti-NCl antibody did not bind to the 24 kD monomer, while the human anti-GBM PPF showed weak binding to the 24 kD and 26 kD monomers.(ABSTRACT TRUNCATED AT 250 WORDS)
Thorner
P
P
Department of Pathology, Hospital for Sick Children, Toronto, Ontario, Canada.
Baumal
R
R
Valli
V E
VE
Mahuran
D
D
McInnes
R
R
Marrano
P
P
D000818:Animals; D001324:Autoantigens / Q000032:analysis*; D001485:Basement Membrane / Q000378:metabolism; D015153:Blotting, Western; D003094:Collagen / Q000032:analysis* / Q000378:metabolism*; D024141:Collagen Type IV; D004283:Dog Diseases / Q000235:genetics* / Q000378:metabolism; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005455:Fluorescent Antibody Technique; D007678:Kidney Glomerulus / Q000378:metabolism*; D008297:Male; D009394:Nephritis, Hereditary / Q000378:metabolism / Q000662:veterinary*; D011863:Radioimmunoassay
D001324:Autoantigens; D024141:Collagen Type IV; C057112:type IV collagen alpha3 chain; D003094:Collagen