title: Characterization of a halothane-resistant strain of measles virus.
abstract: A strain of measles virus (MVr) whose replication demonstrated increased resistance to halothane (2-bromo-2-chloro-1,1,1-trifluoroethane) exposure compared with the susceptible parental strain (MVs) is described. After exposure to a 1.2% halothane concentration, substantial amounts of the measles virus H protein were detected in MVr-infected Vero cell lysates by immunoprecipitation and polyacrylamide gel electrophoresis or by quantitative immunofluorescence staining. The protein was barely detectable in identically treated MVs-infected lysates, however. The recovery of all other measles virus proteins studied was the same in MVr- and MVs-infected cells at this anesthetic concentration. Thus, the altered expression of a single gene product appears to be responsible for the observed halothane resistance.
authors.affiliation: Department of Anesthesiology, University of Michigan Medical Center, Ann Arbor 48109.
authors.forename: E
authors.initials: E
authors.lastname: Bedows
authors.forename: B A
authors.initials: BA
authors.lastname: Davidson
authors.forename: B A
authors.initials: BA
authors.lastname: Williams
authors.forename: P R
authors.initials: PR
authors.lastname: Knight
mesh_terms: D000818:Animals; D004352:Drug Resistance, Microbial; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D006221:Halothane / Q000494:pharmacology*; D008459:Measles virus / Q000032:analysis / Q000187:drug effects*; D014709:Vero Cells; D014764:Viral Proteins / Q000032:analysis
chemical_list: D014764:Viral Proteins; D006221:Halothane