title: Presence of phosphorylated O-ribosyl-adenosine in T-psi-stem of yeast methionine initiator tRNA.
abstract: We report in this paper on isolation and characterization of two unknown nucleosides G* and [A*] located in the T-psi-stem of yeast methionine initiator tRNA, using the combined means of HPLC protocols, real time UV-absorption spectrum, and post-run mass spectrometry by electron impact or fast atom bombardment. The G* nucleoside in position 65 was identified as unmodified guanosine. The structure of the unknown [A*] in position 64 was characterized as an isomeric form of O-ribosyl-adenosine by comparison of its chromatographic, UV-spectral and mass spectrometric properties with those of authentic O-alpha-ribofuranosyl-(1"----2')-adenosine isolated from biosynthetic poly(adenosine diphosphate ribose). Our studies also brought evidence for the presence of a phosphorylmonoester group located on this new modified nucleoside [A*], when isolated by ion exchange chromatography from enzymic hydrolysis of yeast initiator tRNAMet without phosphatase treatment.
authors.affiliation: Laboratoire de Biochimie Médicale, Université de Bourgogne, Dijon, France.
authors.forename: J
authors.initials: J
authors.lastname: Desgrès
authors.forename: G
authors.initials: G
authors.lastname: Keith
authors.forename: K C
authors.initials: KC
authors.lastname: Kuo
authors.forename: C W
authors.initials: CW
authors.lastname: Gehrke
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives* / Q000302:isolation & purification; D002848:Chromatography, DEAE-Cellulose; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D006151:Guanosine / Q000302:isolation & purification; D008715:Methionine / Q000235:genetics*; D009690:Nucleic Acid Conformation; D009705:Nucleosides / Q000302:isolation & purification; D010766:Phosphorylation; D011064:Poly Adenosine Diphosphate Ribose; D012345:RNA, Transfer, Amino Acid-Specific / Q000235:genetics*; D012358:RNA, Transfer, Met / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D009705:Nucleosides; D012345:RNA, Transfer, Amino Acid-Specific; D012358:RNA, Transfer, Met; D006151:Guanosine; D011064:Poly Adenosine Diphosphate Ribose; D008715:Methionine; D000241:Adenosine