Enhanced cell-mediated protection against fatal Escherichia coli septicemia induced by treatment with recombinant IL-2.
Administration of rIL-2 to BALB/c mice induces a rapid, cell-mediated response that is sufficient to protect mice from a lethal i.p. dose of Escherichia coli. Mice were protected from septic death if IL-2 was administered i.p. within 1 h after the bacterial challenge. Optimal protection was provided by treating the lethally challenged mice with rIL-2 at 1 and 5 h or 1, 5, and 10 h after the bacterial challenge and was dose-dependent (greater than or equal to 5.0 x 10(5) U/kg). Furthermore, treatment of mice with anti-IL-2R antibody abolished the protective effect induced by rIL-2 administration. These data suggest that the rIL-2-induced protection against septic death in mice is mediated by a cell type expressing a functional IL-2R. One potentially important therapeutic application of rIL-2 may be to modulate the course of sepsis once the host has been exposed to potentially lethal microbial pathogens.
Goronzy
J
J
Department of Medicine, Stanford University Medical Center, CA 94305.
Weyand
C
C
Quan
J
J
Fathman
C G
CG
O'Hanley
P
P
D000818:Animals; D004306:Dose-Response Relationship, Immunologic; D004334:Drug Administration Schedule; D004927:Escherichia coli Infections / Q000276:immunology / Q000401:mortality / Q000517:prevention & control*; D005260:Female; D007111:Immunity, Cellular / Q000187:drug effects; D007113:Immunity, Innate / Q000187:drug effects*; D007274:Injections, Intraperitoneal; D007376:Interleukin-2 / Q000008:administration & dosage*; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000008:administration & dosage*; D018805:Sepsis / Q000276:immunology / Q000401:mortality / Q000517:prevention & control*
D007376:Interleukin-2; D011994:Recombinant Proteins