A cDNA clone encoding part of the major 25,000-dalton surface membrane antigen of adult Schistosoma mansoni.
Immunoscreening of an adult Schistosoma mansoni cDNA expression library, using antibodies raised against purified adult worm tegumental surface membranes, identified a recombinant clone containing a 141-bp insert. Antibodies raised against the recombinant antigen bound specifically to the tegument of adult worms and immunoprecipitated the major 25,000-dalton surface membrane antigen as well as a 22,000-dalton nascent polypeptide generated by cell-free translation of adult S. mansoni mRNA. The mature 25,000-dalton antigen was found to be precipitated by antibodies from infected mice, rats and humans.
Knight
M
M
Division of Parasitology, National Institute for Medical Research, Mill Hill, London, UK.
Kelly
C
C
Rodrigues
V
V
Yi
X
X
Wamachi
A
A
Smithers
S R
SR
Simpson
A J
AJ
D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D000947:Antigens, Helminth / Q000235:genetics* / Q000276:immunology; D000954:Antigens, Surface / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D012550:Schistosoma mansoni / Q000235:genetics* / Q000276:immunology
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D000954:Antigens, Surface; D004247:DNA