Molybdenum accumulation in chlD mutants of Escherichia coli.
The content of molybdenum in wild-type and chlD cells was measured under a variety of growth conditions to determine if cells with a defective chlD gene were able to accumulate molybdenum. The chlD cells accumulated less molybdenum than wild-type cells did but concentrated molybdenum to a level at least 20-fold higher than the concentration in the culture medium. Molybdenum was present within spheroplasts of chlD cells and was not dialyzable. The chlD cells accumulated as much molybdenum as wild-type cells did when grown in medium containing 0.1 mM molybdate; thus, the capability of incorporation of molybdenum into cellular component(s) was equivalent to that of the wild type under these conditions.
Scott
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Department of Nutritional Sciences, University of California, Berkeley 94720.
Amy
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D003580:Cytochromes / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005796:Genes; D005798:Genes, Bacterial; D008982:Molybdenum / Q000378:metabolism*; D009154:Mutation; D009565:Nitrate Reductases / Q000235:genetics; D013045:Species Specificity; D013104:Spheroplasts / Q000378:metabolism
D003580:Cytochromes; D008982:Molybdenum; D009565:Nitrate Reductases