[Determination of nicotinic acid in injections by high-performance liquid chromatography].
A high-performance liquid chromatographic method for determination of nicotinic acid (NA) in commercial injections was established. NA was determined by high-performance liquid chromatography on an Inertsil ODS column (4.6 x 150 mm) using a mixture of 0.05M monobasic sodium phosphate solution (pH 3) and methanol (80:20) containing 1.5 mM sodium 1-octanesulfonate as the mobile phase at 35 degrees C and the detection wavelength at 260 nm. The working curve for 0 to 120 micrograms nicotinic acid/ml against 100 micrograms caffeine/ml as internal standard passed through the origin and was linear. On comparing the analytical data obtained by this method and those by the colorimetric method, both data were in good agreement.
Tokunaga
H
H
Okada
S
S
Kimura
T
T
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007267:Injections; D000432:Methanol; D009539:Nicotinic Acids / Q000032:analysis*; D010710:Phosphates
D009539:Nicotinic Acids; D010710:Phosphates; C018279:sodium phosphate; D000432:Methanol