Evaluation of a reversed passive latex agglutination test for detection of Escherichia coli heat-labile toxin in culture supernatants.
One hundred strains of Escherichia coli were tested for the production of the heat-labile enterotoxin by the Y1 adrenal cell test and a commercially available reversed passive latex agglutination test. The strains were grown in Casamino Acids-yeast extract broth, and filtered culture supernatants were tested for the presence of heat-labile enterotoxin. There was perfect correlation between the Y1 test and the reversed passive latex agglutination test, and the latter was simple to perform and completed within 48 h.
Scotland
S M
SM
Division of Enteric Pathogens, Central Public Health Laboratory, London, England.
Flomen
R H
RH
Rowe
B
B
D001427:Bacterial Toxins / Q000032:analysis* / Q000276:immunology; D003470:Culture Media; D004768:Enterotoxins / Q000032:analysis* / Q000276:immunology; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins; D007841:Latex Fixation Tests
D001427:Bacterial Toxins; D003470:Culture Media; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C027538:heat-labile enterotoxin, E coli