Cloning and expression of a Clostridium acetobutylicum alpha-amylase gene in Escherichia coli.
A gene library of Clostridium acetobutylicum ATCC824 was constructed in the plasmid vector pEcoR251. The library was tested for the presence of starch hydrolyzing clones. One clone in which the recombinant plasmid, pVP101, conferred alpha-amylase activity to the Escherichia coli host cell, was detected. The gene is carried on a 3.45-kbp BglII restriction fragment. A detailed physical map of pVP101 is presented.
Verhasselt
P
P
F.A. Janssens Memorial Laboratory of Genetics, University of Leuven, Heverlee, Belgium.
Poncelet
F
F
Vits
K
K
Van Gool
A
A
Vanderleyden
J
J
D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003013:Clostridium / Q000201:enzymology / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D000516:alpha-Amylases / Q000032:analysis / Q000235:genetics*
D000516:alpha-Amylases