[A molecular biology technique for the brain--preparation of genomic DNA from the brain].
A simplified method for preparing genomic DNA at and human brain has been established. The procedure includes the following steps: (1) mincing and homogenizing in SSC-EDTA, (2) solubilizing in high concentration detergent (2% SDS or Triton X-100), (3) digesting with proteinase K and RNase A, (4) extracting with phenol-chloroform, (5) finally dialyzing the sample against TE. This method could result in a threefold increase in yield with advantages of simplification and time-saving. The DNA obtained by this method is of a size over 30 kd, and is suitable for the molecular biological and molecular genetical study of brain.
Chen
J
J
D000818:Animals; D015139:Blotting, Southern; D001923:Brain Chemistry; D004247:DNA / Q000302:isolation & purification*; D006801:Humans; D051381:Rats
D004247:DNA