title: Selected phenotypic and cloning properties of a bovine lymphoblastoid cell line, BL20.
abstract: A bovine lymphoblastoid cell line, BL20, was shown to express a BoLA antigen and surface IgM, implying that it was probably of B-cell origin. At low density (less than 10(5)/ml), the cells failed to grow. Inclusion of growth factor-containing supernatants from concanavalin A or pokeweed mitogen (PWM)-activated bovine lymphocytes or from thymus fibroblast-like cells did not improve cloning of the cells. Feeder cells also did not enhance cloning of the cells except for bovine thymus fibroblast-like cell monolayer.
authors.affiliation: International Laboratory for Research on Animal Disease, Nairobi, Kenya.
authors.forename: J O
authors.initials: JO
authors.lastname: Olobo
authors.forename: S J
authors.initials: SJ
authors.lastname: Black
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology; D002417:Cattle; D002455:Cell Division; D002460:Cell Line; D002470:Cell Survival; D002999:Clone Cells; D005347:Fibroblasts; D005455:Fluorescent Antibody Technique; D007136:Immunoglobulins / Q000032:analysis; D010641:Phenotype; D013950:Thymus Gland / Q000166:cytology
chemical_list: D000954:Antigens, Surface; D007136:Immunoglobulins