Artificial cleavage site recognized by plum pox potyvirus protease in Escherichia coli.
A synthetic plum pox virus (PPV) NIb-CP cleavage site was recognized by a PPV protease in an in vivo Escherichia coli expression system. The presence of the natural NIb-CP cleavage site did not affect processing at the artificial one. However, although both the proteases and the cleavage sites of PPV and tobacco etch virus show high sequence homology, a similar cassette from the tobacco etch virus NIb-CP junction was not efficiently recognized by the PPV protease.
García
J A
JA
Centro de Biología Molecular, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Spain.
Riechmann
J L
JL
Laín
S
S
D000595:Amino Acid Sequence; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D015151:Immunoblotting; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases / Q000235:genetics* / Q000378:metabolism; D010942:Plant Viruses / Q000201:enzymology* / Q000235:genetics; D010957:Plasmids; D012689:Sequence Homology, Nucleic Acid
D004279:DNA, Viral; D010447:Peptide Hydrolases