Chromosomal transformation of Escherichia coli recD strains with linearized plasmids.
Wild-type Escherichia coli are resistant to genetic transformation by purified linear DNA, probably in part because of exonuclease activity. We demonstrate that E. coli containing a recD mutation could be easily transformed by linearized plasmids containing a selectable marker. The marker was transferred to the chromosome by homologous recombination, whereas plasmid markers not in the region of homology were lost.
Russell
C B
CB
Institute of Molecular Biology, University of Oregon, Eugene 97403.
Thaler
D S
DS
Dahlquist
F W
FW
D001426:Bacterial Proteins / Q000235:genetics; D002633:Chemotaxis; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D043211:Exodeoxyribonuclease V; D005090:Exodeoxyribonucleases / Q000235:genetics / Q000502:physiology*; D005798:Genes, Bacterial; D009154:Mutation; D011995:Recombination, Genetic; D014170:Transformation, Genetic
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D005090:Exodeoxyribonucleases; D043211:Exodeoxyribonuclease V; C468322:exodeoxyribonuclease V, E coli