Effect of Pseudomonas aeruginosa exotoxin a on PHA-induced lymphocyte proliferation.
P. aeruginosa Exotoxin A induced a dose dependent suppression of PHA-stimulated human lymphocyte proliferation. The suppressor effect was not reversed by interleukin 2 (IL-2) added to the culture medium. Expression of IL-2 receptors (IL-2R) on PHA-blasts a well as their ability for IL-2 binding were decreased by Exotoxin A. Kinetic studies of IL-2 production showed that IL-2 activity was still present in cultures of lymphocytes activated for 72 hours with PHA and Exotoxin A. In contrast, supernatants of lymphocyte cultures activated with PHA and Exotoxin A for 18 hours had low activity of IL-2. Hence, Exotoxin A probably could decrease the utilization of IL-2 due to either inhibition of IL-2R expression or suppression of their ability for IL-2 binding.
Michałkiewicz
J
J
Department of Clinical Immunology, Children's Memorial Hospital, Warszawa.
Patzer
J
J
Dzierzanowska
D
D
Stachowski
J
J
Madaliński
K
K
D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D002478:Cells, Cultured; D005098:Exotoxins / Q000494:pharmacology*; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism / Q000502:physiology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D010835:Phytohemagglutinins / Q000494:pharmacology*; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D005098:Exotoxins; D007376:Interleukin-2; D010835:Phytohemagglutinins; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A