[Measurement of DNA damage in forestomach squamous epithelium by alkaline elution assay].
The alkaline elution assay were applied for assessing DNA damage of forestomach squamous epithelium in the male F344 rat. The animals were starved for 18 hr and then given a single oral dose of the sample in saline or corn oil. After 3 hr the stomach was removed, opened along the greater curvature and washed with cold 0.024M EDTA-0.075M NaCl (pH 7.5). The mucosa of the forestomach was scraped off gently with the edge of a cover glass, and the stratified squamous epithelium was collected by scraping off firmly with the edge of a cover glass. Since the collected epithelium cells adhere to the pipettes, these cells were transferred onto a polycarbonate filter by using a micro spatula. The dose-dependent DNA damages (5 approximately 150 mg/kg) were observed after administration of 1-methyl-3-nitro-1-nitrosoguanidine (MNNG), a forestomach carcinogen.
Morimoto
K
K
Kitsutaka
H
H
Tsuji
K
K
Takahashi
A
A
Furukawa
F
F
Takahashi
M
M
Hayashi
Y
Y
D000818:Animals; D015197:Carcinogenicity Tests / Q000379:methods*; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D004848:Epithelium; D005753:Gastric Mucosa / Q000166:cytology; D008297:Male; D008769:Methylnitronitrosoguanidine / Q000633:toxicity; D051381:Rats; D011916:Rats, Inbred F344
D008769:Methylnitronitrosoguanidine