title: Induction of kinetochore positive and negative micronuclei in V79 cells by the alkylating agent diethylsulphate.
abstract: The induction by diethylsulphate of micronuclei derived from acentric fragments or from whole chromosomes was studied in Chinese hamster V79 cells using autoantibodies from the serum of a scleroderma patient (CREST-syndrome) to detect centromere--kinetochore structures. Centromere-containing micronuclei appeared early after treatment and plateaued both earlier and at lower level than centromere-lacking micronuclei. The frequency of centromere-containing micronuclei was in good agreement with that of mitotic chromosome displacement, suggesting that a high proportion of displaced chromosomes were transmitted to the cytoplasm of one of the two daughter cells, where they gave rise to micronuclei. On the contrary, centromere-lacking micronuclei were more frequent than what could be expected from chromosome fragments observed in mitotic stages.
authors.affiliation: Gesellschaft für Strahlen- und Umweltforschung, Institut für Biophysikalische Strahlenforschung, Frankfurt, FRG.
authors.forename: M
authors.initials: M
authors.lastname: Nüsse
authors.forename: S
authors.initials: S
authors.lastname: Viaggi
authors.forename: S
authors.initials: S
authors.lastname: Bonatti
mesh_terms: D000477:Alkylating Agents / Q000633:toxicity*; D000818:Animals; D000974:Antibodies, Antinuclear; D002455:Cell Division; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects / Q000648:ultrastructure; D004260:DNA Repair / Q000187:drug effects*; D015162:Micronucleus Tests; D009153:Mutagens; D013463:Sulfuric Acid Esters / Q000633:toxicity*; D013464:Sulfuric Acids / Q000633:toxicity*
chemical_list: D000477:Alkylating Agents; D000974:Antibodies, Antinuclear; D009153:Mutagens; D013463:Sulfuric Acid Esters; D013464:Sulfuric Acids; C011612:diethyl sulfate