On the autocrine growth regulation of Morris hepatoma cells.
Chemically induced, transplantable Morris hepatoma 7777 cells (MH) were examined for their anchorage-dependent and anchorage-independent growth properties. MH cells were found to grow on solid surface in a density-dependent manner, with respect to serum factors and gelatinization. The correlation between anchorage independent growth of MH cells and agar or serum concentrations in the culture medium was described. The ability of MH cells to stimulation of soft agar colony formation of NRK-49F indicator cells in coculture assay was presented. Autocrine control of proliferation of MH cells by TGFs-like factor(s) was suggested.
Guzik
K
K
Institute of Molecular Biology, Jagellonian University, Kraków, Poland.
Klein
A
A
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D046508:Culture Techniques / Q000379:methods*; D008114:Liver Neoplasms, Experimental; D051381:Rats; D014407:Tumor Cells, Cultured / Q000166:cytology*