A new Saccharomyces cerevisiae mnn mutant N-linked oligosaccharide structure.
We find that the N-linked Man8GlcNAc2- core oligosaccharide of Saccharomyces cerevisiae mnn mutant mannoproteins is enlarged by the addition of the outer chain to the alpha 1----3-linked mannose in the side chain that is attached to the beta 1----4-linked mannose rather than by addition to the terminal alpha 1----6-linked mannose. This conclusion is derived from structural studies on a phosphorylated oligosaccharide fraction and from mass spectral fragment analysis of neutral core oligosaccharides.
Hernández
L M
LM
Department of Biochemistry, University of California, Berkeley 94720.
Ballou
L
L
Alvarado
E
E
Gillece-Castro
B L
BL
Burlingame
A L
AL
Ballou
C E
CE
D002236:Carbohydrate Conformation; D015180:Electrophoresis, Gel, Two-Dimensional; D009682:Magnetic Resonance Spectroscopy; D009154:Mutation; D009844:Oligosaccharides; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*
D009844:Oligosaccharides