title: In vivo response of microfabricated glucose sensors to glycemia changes in normal rats.
abstract: A planar glucose sensor based on an amperometric detection of H2O2 is presented. The transducer part which is a planar three-electrode cell consisting of two Pt-electrodes and one Ag/AgCl-thin film electrode is realized using microelectronic technology. The overall dimensions are 0.8 mm x 3 mm x 0.38 mm and the working electrode area is 0.1 mm2. GOD is immobilized using glutaraldehyde as a cross-linking agent and bovine serum albumin as a carrier protein. The resulting membrane has a typical thickness of 25 microns. Also, an outer polyurethane membrane is dip-coated all around the tip of the electrode. The influence of pO2 on the sensor response as well as the sensor temperature coefficient, sensitivity and linear range have been investigated. The sensor has been tested in vivo in a subcutaneous tissue of anaesthetised rats. During experiments, blood sampling allows to measure changes in venous plasma glucose using a Beckman analyzer. The sensor response following hyperglycaemic clamps as well as intravenous glucose loads is discussed.
authors.affiliation: LRM, Faculty of Medicine, University of Geneva, Switzerland.
authors.forename: M
authors.initials: M
authors.lastname: Koudelka
authors.forename: F
authors.initials: F
authors.lastname: Rohner-Jeanrenaud
authors.forename: J
authors.initials: J
authors.lastname: Terrettaz
authors.forename: E
authors.initials: E
authors.lastname: Bobbioni-Harsch
authors.forename: N F
authors.initials: NF
authors.lastname: de Rooij
authors.forename: B
authors.initials: B
authors.lastname: Jeanrenaud
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D001786:Blood Glucose / Q000032:analysis*; D005949:Glucose Oxidase; D006943:Hyperglycemia / Q000175:diagnosis*; D008567:Membranes, Artificial; D011140:Polyurethanes; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D001786:Blood Glucose; D008567:Membranes, Artificial; D011140:Polyurethanes; D005949:Glucose Oxidase