title: Phosphorothioates in molecular biology.
abstract: The observation that phosphorothioate analogues of the nucleoside triphosphates are substrates for DNA- and RNA-polymerases has proven a boon for the molecular biologist. As these phosphorothioate-containing polymers are stable to degradation by nucleases and the sulfur atom confers many favourable chemical properties, several applications in molecular biology have been developed, including new methods for site-directed mutagenesis and DNA sequencing.
authors.forename: F
authors.initials: F
authors.lastname: Eckstein
authors.forename: G
authors.initials: G
authors.lastname: Gish
mesh_terms: D004247:DNA / Q000032:analysis; D008967:Molecular Biology; D013873:Thionucleotides / Q000378:metabolism*
chemical_list: D013873:Thionucleotides; D004247:DNA