From analysis to synthesis: new ligand binding sites on the lactate dehydrogenase framework. Part I.
In Part I of this article, the naturally evolved protein framework of lactate dehydrogenase is investigated by genetically introduced modifications which reveal the structural basis of its catalytic and substrate-binding properties. In Part II (to be published in the April issue of TIBS), this analytical information is exploited in the design of two modified forms of the enzyme; one which is specific for a new substrate and one which lacks allosteric regulation.
Clarke
A R
AR
Atkinson
T
T
Holbrook
J J
JJ
D001665:Binding Sites; D007770:L-Lactate Dehydrogenase / Q000096:biosynthesis* / Q000378:metabolism; D008024:Ligands / Q000378:metabolism*; D013329:Structure-Activity Relationship
D008024:Ligands; D007770:L-Lactate Dehydrogenase