Physiological role of ovarian carbonyl reductase and effect of antiestrogen on its activity in rats.
In order to clarify physiological role of carbonyl reductase in rat ovary and effect of antiestrogen on its activity, we investigated effects of five antiestrogens on changes in ovarian enzyme activities towards three carbonyl compounds. All of five antiestrogens tested, significantly decreased 13,14-dihydro-15-ketoprostaglandin F2 alpha 4-benzoylpyridine and menadione reducing activities in ovarian cytosol by both consecutive treatments and single treatment. This inhibition of ovarian enzyme activities by antiestrogen was dramatically antagonized by human chorionic gonadotropin treatment at 3:00 p.m. on the day of proestrus. These results suggest that antiestrogen inhibits LH surge at afternoon of proestrus in rats and that the ovarian enzyme catalyzing reduction of carbonyl compounds is involved in ovulatory process.
Inazu
N
N
Department of Pharmacology and Toxicology, Tokyo College of Pharmacy, Japan.
Inaba
N
N
Kogo
H
H
Satoh
T
T
D000429:Alcohol Oxidoreductases / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000502:physiology*; D000449:Aldehyde Reductase; D000074408:Aldo-Keto Reductases; D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004965:Estrogen Antagonists / Q000494:pharmacology*; D005260:Female; D010053:Ovary / Q000187:drug effects / Q000201:enzymology*; D051381:Rats; D011921:Rats, Inbred WKY
D006063:Chorionic Gonadotropin; D004965:Estrogen Antagonists; D000429:Alcohol Oxidoreductases; D000074408:Aldo-Keto Reductases; D000449:Aldehyde Reductase