Cloning, sequence and expression in Escherichia coli of cDNA for ovine pregrowth hormone.
cDNA prepared from mRNA from ovine anterior pituitary glands was cloned in Escherichia coli and the sequence of a clone encoding the full coding sequence of ovine pregrowth hormone (preGH) determined. The predicted sequence for ovine GH agrees with that determined previously on the protein, except that residue 99 is asparagine rather than aspartic acid. The cDNA sequence also accords with one of the two genomic sequences for the ovine GH gene that have been reported. Expression plasmids using trp and lac promoters were constructed which allowed expression at low levels of ovine preGH in E. coli, as detected by immunoblotting and immunoassay.
Warwick
J M
JM
Biochemistry Laboratory, School of Biological Sciences, University of Sussex, Brighton U.K.
Wallis
O C
OC
Wallis
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D004926:Escherichia coli / Q000235:genetics*; D013006:Growth Hormone / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000096:biosynthesis / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D012756:Sheep
D011498:Protein Precursors; D011993:Recombinant Fusion Proteins; C029149:pregrowth hormone; D013006:Growth Hormone; D004247:DNA