title: Characterization of human mammary cell types in primary culture: immunofluorescent and immunocytochemical indicators of cellular heterogeneity.
abstract: Parenchymal organoidal structures that were obtained from collagenase digestion of reduction mammoplasty specimens of apparently normal human breasts have been grown in short-term primary cultures, either on plastic or on floating gels of polymerized rat-tail collagen. Three morphologically distinct major cell types are readily observed in both systems: cuboidal cells, which occupy apical positions on collagen gels; larger, epithelioid, or basal cells on gels; and elongated cells which penetrate into the gel. In addition, a fourth cell type, that of large, flat cell, is observed less readily by phase contrast microscopy on the surface of cultures grown on plastic. Immunofluorescent and immunocytochemical staining of cultures on plastic or histologic sections of cultures on gels have been undertaken with antisera and other histochemical reagents that stain the different parenchymal cell types in vivo. Thus antisera to epithelial membrane antigen(s), monoclonal antibodies (MABs) to the defatted mammary milk fat globule membrane, peanut lectin, and keratin MAB LE61, which preferentially stain the epithelial cells of ducts in vivo, also stain the cuboidal/apical cells in vitro. The large, flat cells are stained intensely by the first three reagents but not by the last one. Antisera to collagen IV, laminin, fibronectin, actin, keratin MAB LP34, MABs to the common acute lymphoblastic leukemia antigen, and MAB LICR-LON-23.10, which showed enhanced staining for the ductal myoepithelial cells in vivo, also stain the epithelioid/elongated cells in vitro. However, the effect of the last four reagents is reduced considerably in most elongated cells, and MAB LP34 stains the large, flat cells intensely. Heterogeneous cells of intermediate morphologies and staining patterns between the cuboidal cells and large, flat cells are related to mammary epithelial cells. whereas the large epithelioid/elongated cells have some characteristics of myoepithelial cells, and that intermediate forms may exist in culture between the two parenchymal cell types.
authors.affiliation: Department of Biochemistry, University of Liverpool, United Kingdom.
authors.forename: P S
authors.initials: PS
authors.lastname: Rudland
authors.forename: C M
authors.initials: CM
authors.lastname: Hughes
authors.forename: S A
authors.initials: SA
authors.lastname: Ferns
authors.forename: M J
authors.initials: MJ
authors.lastname: Warburton
mesh_terms: D000328:Adult; D001940:Breast / Q000166:cytology*; D002478:Cells, Cultured; D003094:Collagen; D003598:Cytoskeletal Proteins / Q000378:metabolism; D004847:Epithelial Cells; D005260:Female; D005455:Fluorescent Antibody Technique; D005782:Gels; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D009940:Organoids / Q000166:cytology; D010969:Plastics
chemical_list: D003598:Cytoskeletal Proteins; D005782:Gels; D006023:Glycoproteins; D010969:Plastics; D003094:Collagen