title: Regulation of glucose transporter messenger RNA in insulin-deficient states.
abstract: Recent studies have indicated that a family of structurally related proteins with distinct but overlapping tissue distributions are responsible for facilitative glucose transport in mammalian tissues. Insulin primarily stimulates glucose transport by inducing the redistribution of a unique glucose transporter protein from an intracellular pool to the plasma membrane. This 509-amino-acid integral membrane protein, termed GLUT-4, is the main insulin-responsive glucose transporter in adipose and muscle tissues. We have observed a dramatic decrease (tenfold) in the steady-state levels of GLUT-4 messenger RNA in adipose tissue from fasted rats or rats made insulin deficient with streptozotocin. Insulin treatment of the streptozotocin-diabetic rats or refeeding the fasted animals causes a rapid recovery of the GLUT-4 mRNA to levels significantly above those observed in untreated control animals. By contrast, the levels of the erythrocyte/HepG2/rat brain-type glucose transporter mRNA remain essentially unchanged under these conditions. These data suggest that the in vivo expression of GLUT-4 mRNA in rat adipose tissue is regulated by insulin.
authors.affiliation: Department of Physiology & Biophysics, University of Iowa, Iowa City 52242.
authors.forename: W I
authors.initials: WI
authors.lastname: Sivitz
authors.forename: S L
authors.initials: SL
authors.lastname: DeSautel
authors.forename: T
authors.initials: T
authors.lastname: Kayano
authors.forename: G I
authors.initials: GI
authors.lastname: Bell
authors.forename: J E
authors.initials: JE
authors.lastname: Pessin
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005215:Fasting; D005786:Gene Expression Regulation; D007328:Insulin / Q000502:physiology*; D009004:Monosaccharide Transport Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D001786:Blood Glucose; D007328:Insulin; D009004:Monosaccharide Transport Proteins; D012333:RNA, Messenger