[Creation and analysis of a bank of chromosomal genes of Streptococcus group A].
The representative genomic library of chromosomal genes has been constructed for streptococcus group A serotype M48 strain 1/64 on the vector lambda L 47.1. Screening of the obtained genomic library by hybridization and immunological techniques revealed about 50 clones producing the streptococcal antigens (extracellular nonidentified products and non-type specific structural streptococcal proteins). Among the recombinant clones three were found to harbour the genetic determinants for M-protein. One the clones contains a determinant coding for epitopes crossreacting with antisera to M-proteins of other serotypes and a protective epitope. The presence of the latter was tested in an indirect bactericidal test.
Nesterchuk
L B
LB
Gupalova
T V
TV
Kolesnichenko
T G
TG
Suvorov
A N
AN
Golubkov
V I
VI
D000818:Animals; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000235:genetics; D002352:Carrier Proteins; D005798:Genes, Bacterial; D007256:Information Systems; D009895:Opsonin Proteins / Q000235:genetics; D011817:Rabbits; D013297:Streptococcus pyogenes / Q000235:genetics*
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D009895:Opsonin Proteins; C014689:streptococcal M protein
Sozdanie i analiz banka khromosomnykh genov Streptokokka gruppy A.