The hepatic asialoglycoprotein receptor selectively binds to some endogenous tissues.
The hepatic asialoglycoprotein receptor (ASGP-R) was isolated from various rat tissues or freshly prepared single cell suspensions and tested for the binding to endogenous tissues or specific cell types by indirect immunofluorescence. Inhibition with N-acetyl-D-galactosamine demonstrated specificity of binding. ASGP-R binds to mesodermal tissues and to selected cells of the majority of glandular tissues but not to lining epithelia. ASGP-R stains heart muscle but not skeletal muscle. In addition, ASGP-R stains spleen cells (52%), bone marrow cells (55%), thymocytes (62%), and a fraction of peripheral blood lymphocytes (29%), which was identified as B-lymphocytes. Five different rat tumors also showed binding of ASGP-R. The binding pattern and staining intensity of peanut agglutinin and soybean agglutinin were strikingly different although the binding specificity of these lectins is related to the ASPG-R. It is concluded that considerable numbers of endogenous binding sites for the hepatic ASGP-R exist in normal tissue, even on cells which pass the liver on circulation.
Treichel
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U
Diabetes Research Institute, Düsseldorf/Federal Republic of Germany.
Roos
P H
PH
Kolb
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H
D000818:Animals; D037263:Asialoglycoprotein Receptor; D001853:Bone Marrow / Q000378:metabolism; D003238:Connective Tissue / Q000378:metabolism*; D003239:Connective Tissue Cells; D005455:Fluorescent Antibody Technique; D005753:Gastric Mucosa / Q000378:metabolism; D006001:Glycoconjugates / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D037102:Lectins / Q000378:metabolism; D008099:Liver / Q000032:analysis* / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D008648:Mesoderm / Q000166:cytology / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism; D019887:Peanut Agglutinin; D037121:Plant Lectins; D011467:Prostate / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011971:Receptors, Immunologic / Q000032:analysis / Q000378:metabolism*; D012469:Salivary Glands / Q000378:metabolism; D030262:Soybean Proteins; D013154:Spleen / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism; D013961:Thyroid Gland / Q000378:metabolism; D001743:Urinary Bladder / Q000378:metabolism; D010835:Phytohemagglutinins
D037263:Asialoglycoprotein Receptor; D006001:Glycoconjugates; D037102:Lectins; D019887:Peanut Agglutinin; D037121:Plant Lectins; D011971:Receptors, Immunologic; D030262:Soybean Proteins; C028824:soybean lectin; D010835:Phytohemagglutinins