Expression of interleukin-2 receptor beta subunit in hematopoietic malignancies.
The expression of the interleukin-2 (IL-2) receptor was studied in neoplastic cells derived from acute leukemias, T-cell lymphoblastic lymphomas, peripheral T-cell lymphomas, chronic lymphocytic leukemias, well-differentiated lymphocytic lymphomas, and established cell lines by both flow cytometric analysis and sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS-PAGE) after affinity crosslinking of radiolabeled IL-2. Cells from most acute leukemias (19 of 22), irrespective of their subtype (T, common or nonlymphoid leukemias), as well as T-cell lymphoblastic lymphomas and peripheral T-cell lymphomas expressed only the p70-75 beta subunit of the IL-2 receptor. Cells from the more mature B-cell neoplasms, chronic lymphocytic leukemia, and well-differentiated lymphocytic lymphoma, expressed predominantly alpha beta IL-2 receptors (11 of 14). In contrast to these results, most cell lines established from hematopoietic malignancies do not express either chain of the IL-2 receptor. Further studies are necessary to determine the exact function of the IL-2R p70-75 beta subunit in immature hematopoietic cells, but its wide distribution throughout the hematopoietic system suggests that IL-2 may play a role in the early stages of hematopoiesis.
Rosolen
A
A
Pediatric Branch, NCI, Bethesda, MD 20892.
Nakanishi
M
M
Poplack
D G
DG
Cole
D
D
Quinones
R
R
Reaman
G
G
Trepel
J B
JB
Cotelingam
J D
JD
Sausville
E A
EA
Marti
G E
GE
D002460:Cell Line; D003432:Cross-Linking Reagents; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism; D007938:Leukemia / Q000145:classification / Q000378:metabolism*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000032:analysis / Q000378:metabolism; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000378:metabolism; D008223:Lymphoma / Q000032:analysis* / Q000145:classification; D010641:Phenotype; D015375:Receptors, Interleukin-2 / Q000032:analysis* / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000378:metabolism
D003432:Cross-Linking Reagents; D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D011994:Recombinant Proteins