Chicago sky blue and a helium neon laser abolish endothelium dependent relaxation in vivo in the microcirculation.
Chicago sky blue, also known as Niagara sky blue, is a vital dye that can successfully be used as an intravascular energy absorbing target for the light from a helium-neon (HeNe) laser. The result of this light/dye interaction is endothelium damage which can be controlled by adjusting the duration of the laser exposure and the amount of dye injected intravenously. The endothelial damage probably is the result of the heat generated by the dyes absorption of energy at the interface between plasma and endothelium. The most minimal damage resulted in selective loss of the dilation normally produced by acetylcholine and bradykinin, two endothelium dependent dilators. The dilation produced by sodium nitroprusside, a dilator acting directly on vascular smooth muscle, was preserved. More severe injury (i.e. more prolonged exposure to light and/or more dye, resulted in local platelet aggregation at the site of laser impact.
Nishimura
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Department of Pathology, Neuropathology, Medical College of Virginia, Richmond 23298-0017.
Nelson
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GH
Rosenblum
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WI
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001391:Azo Compounds / Q000633:toxicity*; D001920:Bradykinin / Q000494:pharmacology; D004396:Coloring Agents / Q000633:toxicity*; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000528:radiation effects; D007834:Lasers / Q000009:adverse effects*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008833:Microcirculation / Q000187:drug effects* / Q000528:radiation effects; D009599:Nitroprusside / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects / Q000528:radiation effects; D014343:Trypan Blue; D014664:Vasodilation / Q000187:drug effects* / Q000528:radiation effects
D001391:Azo Compounds; D004396:Coloring Agents; D009599:Nitroprusside; C009000:pontamine sky blue; D014343:Trypan Blue; D000109:Acetylcholine; D001920:Bradykinin