GTP-dependent Ca2+ release from rat liver microsomes. Vesicle fusion is not required.
The GTP-dependent calcium release from rat liver microsomes is known to be promoted in the presence of colloids like polyethyleneglycol (PEG), polyvinylpyrrolidine, or albumin. Dawson et al. [(1987) Biochem. J. 244, 87-92] using the 'fusogen' PEG have concluded that both GTP-induced calcium efflux and the enhancement of InsP3-promoted calcium release in the presence of GTP could be attributed to a GTP-dependent vesicle fusion. Here, using the more physiological colloid albumin we report that GTP-induced calcium release from rat liver microsomes may not be linked to vesicle fusion.
Kleineke
J
J
Abteilung für Klin, Biochemie, Zentrum Innere Medizin der Universität, Göttingen, FRG.
Schröder
A
A
Söling
H D
HD
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology*; D008027:Light; D008297:Male; D008561:Membrane Fusion; D008854:Microscopy, Electron; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D011092:Polyethylene Glycols / Q000494:pharmacology; D011205:Povidone / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012542:Scattering, Radiation; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013873:Thionucleotides / Q000494:pharmacology
D013873:Thionucleotides; D012710:Serum Albumin, Bovine; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D011092:Polyethylene Glycols; D006160:Guanosine Triphosphate; D011205:Povidone; D002118:Calcium