title: Evidence for a pertussis toxin-sensitive G protein involved in the control of meiotic reinitiation of Xenopus laevis oocytes.
abstract: Meiotic reinitiation of Xenopus laevis oocytes is induced in vitro by progesterone or insulin. The hormonal effect is inhibited in a dose-dependent manner by the injection of the A protomer of pertussis toxin (islet-activating protein, IAP) into the oocytes. This inhibition occurs only before the appearance of a maturation-promoting activity in the cytoplasm. Furthermore, injection of the toxin A protomer into recipient oocytes does not inhibit the induction of maturation obtained through injection of cytoplasm containing the maturation-promoting factor. The inhibition effect of the toxin A protomer is reversible with time. These results suggest that a pertussis-sensitive G protein is involved in intracellular signaling systems leading to the induction of MPF activity.
authors.affiliation: Department of Obstetrics and Gynecology, Hôpital Cantonal Universitaire, Geneva, Switzerland.
authors.forename: V
authors.initials: V
authors.lastname: Pellaz
authors.forename: S
authors.initials: S
authors.lastname: Schorderet-Slatkine
mesh_terms: D000818:Animals; D005260:Female; D007328:Insulin / Q000494:pharmacology; D008540:Meiosis / Q000187:drug effects; D009865:Oocytes / Q000166:cytology*; D009866:Oogenesis / Q000187:drug effects; D037342:Pertussis Toxin; D011374:Progesterone / Q000494:pharmacology; D010566:Virulence Factors, Bordetella / Q000494:pharmacology / Q000502:physiology*; D014982:Xenopus laevis / Q000502:physiology*
chemical_list: D007328:Insulin; D010566:Virulence Factors, Bordetella; D011374:Progesterone; D037342:Pertussis Toxin