title: Alterations in dermal collagen in ultraviolet irradiated hairless mice.
abstract: Chronic exposure of the skin to sunlight results in severe dermal connective tissue damage that is characterized by the basophilic degeneration of collagen and the accumulation of an elastotic material. The aim of this study was to identify changes in collagen (the major structural protein of the skin) in ultraviolet irradiated mouse skin using immunochemical and biochemical techniques. Specific antibodies directed against the aminopropeptide of type III procollagen were used in immunofluorescence and immunoblotting studies. Immunofluorescent staining of irradiated and nonirradiated mice skin showed that the aminopropeptide of type III procollagen was distributed throughout the dermis in a pattern similar to that observed for type I collagen. Extracts of irradiated (5 and 10 weeks) and nonirradiated skins were then subjected to immunoblotting techniques. Levels of pN alpha 1, type III procollagen (measured by radioimmunoassay) were reduced in the extracts prepared from skins of mice that were irradiated for 5 and 10 weeks. Immunoelectron microscopy verified the loss of pN alpha 1 type III procollagen in irradiated skin. Collagen fibers of nonirradiated skin demonstrated normal labeling with antibody directed against the aminopropeptide of type III procollagen. In contrast, collagen fibers of 10 week irradiated skin failed to label with this antibody. The pN alpha 1 type III collagen is known to coat type I collagen fibers of normal skin. Therefore, its absence from the surface of type I collagen fibers of irradiated skin may play a role in the development of the elastotic material.
authors.affiliation: Department of Dermatology, Mount Sinai School of Medicine, New York, New York 10029.
authors.forename: E
authors.initials: E
authors.lastname: Schwartz
authors.forename: F A
authors.initials: FA
authors.lastname: Cruickshank
authors.forename: J S
authors.initials: JS
authors.lastname: Perlish
authors.forename: R
authors.initials: R
authors.lastname: Fleischmajer
mesh_terms: D000818:Animals; D003094:Collagen / Q000145:classification / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D051379:Mice; D008854:Microscopy, Electron; D010455:Peptides / Q000378:metabolism; D011347:Procollagen / Q000378:metabolism; D012867:Skin / Q000378:metabolism* / Q000528:radiation effects / Q000648:ultrastructure; D014466:Ultraviolet Rays
chemical_list: D010455:Peptides; D011347:Procollagen; D003094:Collagen