Relevance of complotyping and subtyping of MHC class I gene products in haplotype definition for allogeneic bone marrow transplantation.
In preparation for a bone marrow transplantation 217 patients and their families were complotyped for Bf, C4A and C4B in addition to the routinely performed HLA-A,B,C,DR and HLA-D typing. In 147 families uncertainties in haplotype definition occurred which could be solved in 37 cases (25%) by complotyping. Additionally, patients and their relatives were subtyped for class I gene products by one-dimensional isoelectric focusing, a method by which serologically identical HLA-A, B, or C antigens could be split in five out of 22 cases tested. The results obtained clearly show the relevance of both methodologies for finding the best match of donor/recipient pairs to help to prevent MHC-induced graft-versus-host disease after bone marrow transplantation.
Doxiadis
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G
Institute of Immunogenetics, University Hospital of Essen, FRG.
Doxiadis
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I
Frenz
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Vögeler
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U
Grosse-Wilde
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H
D016026:Bone Marrow Transplantation; D003181:Complement C4 / Q000032:analysis / Q000235:genetics; D015934:Complement C4a; D015935:Complement C4b; D011415:Complement Factor B / Q000032:analysis / Q000235:genetics; D005805:Genes, MHC Class I; D006086:Graft vs Host Disease / Q000517:prevention & control; D006680:HLA Antigens / Q000032:analysis / Q000235:genetics; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D015151:Immunoblotting; D007525:Isoelectric Focusing; D014184:Transplantation, Homologous
D003181:Complement C4; D006680:HLA Antigens; D015934:Complement C4a; D015935:Complement C4b; D011415:Complement Factor B