Increase in gene expression by respiratory-deficient mutation.
Respiratory-deficient mutants (rho- cells) of Saccharomyces cerevisiae produced about 10 times as much human (h-) lysozyme as did wild-type strains (rho+ cells) when the GAL10 promoter was used in an expression plasmid with the h-lysozyme gene. Introduction of intact mitochondria into the rho- cells resulted in a significant decrease in the production of h-lysozyme, indicating that the rho- mutation increased the expression of the h-lysozyme gene. The copy number of the expression plasmid was not responsible for the increased expression. The level of h-lysozyme mRNA in the rho- cells was also much higher than that in the rho+ cells especially at the stationary phase. The increased expression of the h-lysozyme gene was also observed when a glyceraldehyde-3-phosphate dehydrogenase gene promoter and the PHO5 promoter were used in the expression plasmid. The rho- mutation also increased the expression of the PHO5 gene under the control of the HIS5 promoter in a plasmid and the ACT1 gene in the yeast chromosome, but did not increase the expression of the ribosomal RNA gene. In contrast to the rho- mutants, pet mutants did not show higher gene expression compared with wild-type strains.
Kaisho
Y
Y
Central Research Division, Takeda Chemical Industries Ltd., Osaka, Japan.
Yoshimura
K
K
Nakahama
K
K
D015152:Blotting, Northern; D005786:Gene Expression Regulation; D009113:Muramidase / Q000096:biosynthesis / Q000235:genetics*; D009154:Mutation; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012331:RNA, Fungal / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D014170:Transformation, Genetic
D012331:RNA, Fungal; D012333:RNA, Messenger; D009113:Muramidase