title: A monoclonal antibody to a synthetic fragment of rabies virus glycoprotein binds ligands of the nicotinic cholinergic receptor.
abstract: Rabies virus glycoprotein and snake venom curaremimetic neurotoxins share a region of high homology (30-45 for neurotoxins and 190-203 for the glycoprotein) in the regions that are believed to be responsible for binding the nicotinic acetylcholine receptor. Monoclonal antibodies raised to the 190-203 synthetic fragment of rabies virus glycoprotein were immobilized on a high performance affinity chromatography column and were able to bind neurotoxins. Toxins were displaced from the affinity column by elution at acidic pH and by affinity competition with acetylcholine at neutral pH. Furthermore, the affinity column proved to be useful for the purification of cholinergic ligands. Overall, these results indicate that the paratope of our monoclonal antibodies could behave as an 'internal image' of the nicotinic cholinergic receptor acetylcholine binding site.
authors.affiliation: CRISMA, Policlinico Le Scotte, Siena, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Rustici
authors.forename: A
authors.initials: A
authors.lastname: Santucci
authors.forename: L
authors.initials: L
authors.lastname: Lozzi
authors.forename: S
authors.initials: S
authors.lastname: Petreni
authors.forename: A
authors.initials: A
authors.lastname: Spreafico
authors.forename: P
authors.initials: P
authors.lastname: Neri
authors.forename: L
authors.initials: L
authors.lastname: Bracci
authors.forename: P
authors.initials: P
authors.lastname: Soldani
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D001667:Binding, Competitive; D002846:Chromatography, Affinity; D004546:Elapid Venoms / Q000378:metabolism*; D006023:Glycoproteins / Q000276:immunology*; D008024:Ligands; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000378:metabolism*; D010446:Peptide Fragments / Q000276:immunology; D011820:Rabies virus / Q000276:immunology*; D011978:Receptors, Nicotinic / Q000378:metabolism*; D012910:Snake Venoms / Q000378:metabolism; D014764:Viral Proteins / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D004546:Elapid Venoms; D006023:Glycoproteins; D008024:Ligands; D009498:Neurotoxins; D010446:Peptide Fragments; D011978:Receptors, Nicotinic; D012910:Snake Venoms; D014764:Viral Proteins