[Cloning and study of Corynebacterium glutamicum genes complementing ilvA and thrA2 mutations in Escherichia coli].
Molecular cloning and expression of Corynebacterium glutamicum genes complementing Escherichia coli mutations thrA2 and ilvA was performed. It was demonstrated that the thrA2 gene of C. glutamicum is located close to thrB on EcoRI DNA fragment 4.1 kb long. The fragment was cloned in pUC18 vector. The thrA2 gene is expressed in the recombinant plasmid pOBT3 under control of the vector pUC18 Plac promoter. In E. coli minicells, the genes thrA2 and thrB determined synthesis of proteins of Mr 43kD and 25 kD, respectively. A gene complementing ilvA mutation of E. coli was identified in a library of EcoRI C. glutamicum DNA fragments. This library was constructed using plasmid vector. It was shown that the ilvA gene of C. glutamicum is located inside the 3.6 kb EcoRI fragment and is expressed using its own promoter.
Beskrovnaia
O Iu
OIu
Bukanov
N O
NO
Okorokov
A L
AL
Fonshteĭn
M Iu
MIu
Iankovskiĭ
N K
NK
D003001:Cloning, Molecular; D003352:Corynebacterium / Q000235:genetics*; D004247:DNA / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D005822:Genetic Vectors; D009154:Mutation; D009693:Nucleic Acid Hybridization; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D014161:Transduction, Genetic
D004247:DNA
Klonirovanie i izuchenie genov Corynebacterium glutamicum, komplementiruiushchikh mutatsii ilvA i thrA2 Escherichia coli.