title: Effects of irradiation and tunicamycin on the surface glycoproteins of Schistosoma mansoni.
abstract: The cercarial glycocalyx and schistosomulum surface contains a number of glycoproteins which are expressed in very variable amounts within a parasite population. Tunicamycin inhibits glycoprotein synthesis of schistosomula if the parasites are incubated for 24 hr with the drug (10 micrograms ml-1). An unexpected increase in lectin binding to the parasite surface was observed but no other changes were detected. Schistosomula treated in this way did not develop in the host past the lung stage. Ultraviolet irradiation (400 microW min cm-2) also inhibited glycoprotein synthesis. Synthesis of other proteins, and in particular heat shock proteins, were also inhibited. Sera from mice (NIH strain) infected with irradiated cercariae contained antibodies which bound to normal schistosomula with lower affinity than to irradiated parasites. This is evidence that irradiation modifies the surface and secreted glycoproteins of schistosomula, so they are processed in a different way to normal glycoproteins by the host's immune system. The effects of irradiation on heat shock protein synthesis may allow the parasite to release a variety of proteins and glycoproteins in abnormal conformations. This may explain the enhanced immunogenicity of irradiated cercariae.
authors.affiliation: University of Glasgow.
authors.forename: J R
authors.initials: JR
authors.lastname: Kusel
authors.forename: A
authors.initials: A
authors.lastname: Wales
authors.forename: L
authors.initials: L
authors.lastname: Vieira
authors.forename: K Y
authors.initials: KY
authors.lastname: Wu
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology* / Q000528:radiation effects; D000954:Antigens, Surface / Q000276:immunology / Q000528:radiation effects; D002241:Carbohydrates / Q000276:immunology / Q000528:radiation effects*; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000187:drug effects* / Q000276:immunology / Q000528:radiation effects*; D012550:Schistosoma mansoni / Q000276:immunology*; D014415:Tunicamycin / Q000494:pharmacology*
chemical_list: D000947:Antigens, Helminth; D000954:Antigens, Surface; D002241:Carbohydrates; D008562:Membrane Glycoproteins; D014415:Tunicamycin