T-cell mitogenesis stimulates the synthesis of a mRNA species coding for a 43-kDa peptide reactive with CM-H-9, a monoclonal antibody specific for placental isoferritin.
In studying the changes that occur in concanavalin A-activated T lymphocytes, an mRNA species was discovered by hybridization of poly(A)+ mRNA with a human ferritin heavy chain cDNA probe. This ferritin mRNA, termed superheavy chain mRNA, differed from the known human ferritin heavy chain mRNAs by its larger size and degree of homology. The superheavy chain mRNA was isolated by sucrose-gradient centrifugation and translated in vitro in a cell-free system. The products obtained included two peptides (superheavy) of 43kDa that reacted with CM-H-9, a monoclonal antibody specific for placental isoferritin. De novo synthesis in intact transformed T cells revealed the synthesis of the superheavy chain peptides that were immunoprecipitated by anti-ferritin monoclonal antibody CM-G-8 and by placental isoferritin specific monoclonal antibody CM-H-9. The above results indicated that blast transformation of human T cells stimulated the appearance of a unique mRNA species that coded for a superheavy chain peptide associated with placental isoferritin, which was not detected in resting T cells.
Moroz
C
C
Rogoff Medical Research Institute, Beilinson Medical Center, Petah Tikva, Israel.
Shterman
N
N
Kupfer
B
B
Ginzburg
I
I
D000911:Antibodies, Monoclonal; D002499:Centrifugation, Density Gradient; D003208:Concanavalin A / Q000494:pharmacology; D015342:DNA Probes; D003609:Dactinomycin / Q000494:pharmacology; D005293:Ferritins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D007163:Immunosorbent Techniques; D008213:Lymphocyte Activation; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D010920:Placenta / Q000032:analysis*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D015342:DNA Probes; D012333:RNA, Messenger; D003208:Concanavalin A; D003609:Dactinomycin; D005293:Ferritins