Activated c-N-ras in radiation-induced acute nonlymphocytic leukemia: twelfth codon aspartic acid.
We describe the detection and characterization of an activated c-N-ras allele from a gamma-radiation-induced canine acute nonlymphocytic leukemia (ANLL). The activated allele was detected by use of the NIH3T3 transfection/transformation assay. The leukemia DNA had a transforming activity of 0.0125 foci/microgram. By the use of a double anti-ras antibody enzyme-linked immunoblot assay, we have dissected the lesion within the activated c-N-ras allele. Aspartic acid has been substituted for the normal glycine at position 12 in the activated p21c-N-ras. The expression of the mutant p21ras has also been detected in an in vivo passage of the radiation-induced canine ANLL from which the activated c-N-ras allele was isolated. We have demonstrated sufficient homology between canine c-N-ras genes and the human cDNA c-N-ras clone, p6a1, that allows this probe to be used in Southern blotting of canine tissues. In addition, anti-ras antibodies generated against both murine and human ras antigens are capable of detecting canine p21ras species.
Gumerlock
P H
PH
Department of Internal Medicine, University of California-Davis Medical Center, Sacramento 95817.
Meyers
F J
FJ
Foster
B A
BA
Kawakami
T G
TG
deVere White
R W
RW
D000483:Alleles; D000818:Animals; D001224:Aspartic Acid / Q000235:genetics*; D003037:Cobalt Radioisotopes; D003062:Codon / Q000235:genetics*; D004285:Dogs; D005720:Gamma Rays; D005786:Gene Expression Regulation; D011905:Genes, ras; D015470:Leukemia, Myeloid, Acute / Q000209:etiology / Q000235:genetics*; D007953:Leukemia, Radiation-Induced / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*
D003037:Cobalt Radioisotopes; D003062:Codon; D012333:RNA, Messenger; D001224:Aspartic Acid