title: Effects of periodontopathic bacteria on IL-1 and IL-1 inhibitor production by human polymorphonuclear neutrophils.
abstract: The effect of heat-killed periodontopathic bacteria on the production of interleukin-1 (IL-1) and an IL-1 inhibitor by human polymorphonuclear neutrophils (PMN's) was examined. Peripheral blood was obtained from 18 healthy volunteers and the PMN's were separated using dextran sedimentation and Ficoll-Paque density gradient centrifugation. The PMN's (5 x 10(5) cells/well) were cultured in serum-free media with or without heat-killed periodontopathic bacteria. Four gram-negative periodontopathic bacteria were used; Bacteroides gingivalis FDC 381, Bacteroides forsythus FDC 338, Actinobacillus actinomycetemcomitans Y4 and Fusobacterium nucleatum FDC 263. The non-oral Fusobacterium mortiferum ATCC 25557 was used as a control organism. IL-1 activity was assayed using thymocyte proliferation. The non-oral organism F. mortiferum stimulated IL-1 production by PMN's, in contrast none of the periodontopathic bacteria stimulated IL-1 release although the bacteria themselves had an IL-1 enhancing effect. Following fractionation of the periodontopathic bacteria stimulated PMN supernatants, an IL-1 inhibitory fraction was identified. These results may illustrate a further mechanism by which periodontopathic bacteria may evade the protective effect of PMN's and may also suggest a regulatory role for PMN's in chronic inflammatory periodontal disease.
authors.forename: K
authors.initials: K
authors.lastname: Yamazaki
authors.forename: B
authors.initials: B
authors.lastname: Polak
authors.forename: P S
authors.initials: PS
authors.lastname: Bird
authors.forename: E
authors.initials: E
authors.lastname: Gemmell
authors.forename: K
authors.initials: K
authors.lastname: Hara
authors.forename: G J
authors.initials: GJ
authors.lastname: Seymour
mesh_terms: D000190:Actinomyces; D001439:Bacteroides; D005673:Fusobacterium; D006131:Growth Inhibitors / Q000096:biosynthesis; D006801:Humans; D007375:Interleukin-1 / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D008222:Lymphokines / Q000096:biosynthesis*; D009504:Neutrophils / Q000378:metabolism*; D010510:Periodontal Diseases / Q000382:microbiology*
chemical_list: D006131:Growth Inhibitors; D007375:Interleukin-1; D008222:Lymphokines