Sodium butyrate induces major morphological changes in C6 glioma cells that are correlated with increased synthesis of a spectrin-like protein.
Butyrate induced flattening and development of cell processes in rat glioma (C6) cells and this change was correlated with an increase in the synthesis of a polypeptide doublet with an apparent molecular weight of about 200 kDa. Blot analysis revealed that at least one of these polypeptides was a spectrin-like protein. Indirect immunofluorescence studies with the spectrin antiserum indicated that the antigen was present in the cell bodies, and also in the cell processes. Thus fodrin may be one the major targets for the action of butyrate on C6 cells.
Hargreaves
A J
AJ
Unidad de Endocrinologia Experimental, Instituto de Investigaciones Biomedicas, Madrid, Spain.
Yusta
B
B
Avila
J
J
Hesketh
J E
JE
Aranda
A
A
Pascual
A
A
D001345:Autoradiography; D015153:Blotting, Western; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D002352:Carrier Proteins / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D005910:Glioma / Q000378:metabolism / Q000473:pathology*; D008840:Microfilament Proteins / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D013049:Spectrin
D002087:Butyrates; D020148:Butyric Acid; D002352:Carrier Proteins; D008840:Microfilament Proteins; D013049:Spectrin