Creatine kinase BB isozyme in the blood during open heart surgery--comparison with creatine kinase MB and MM isozymes.
By using a recently developed highly sensitive enzyme immunoassay method, concentrations of the 3 forms of cytoplasmic creatine kinases (CK-BB, CK-MB and CK-MM) were determined in blood samples serially taken from 18 patients who received mitral valve replacement. Blood CK-BB levels, 0.64 +/- 0.32 ng/ml at the beginning of anesthesia, rose sharply after reperfusion reaching the peak level (23.3 +/- 7.56 ng/ml) 2 hours after reperfusion, and then fell rapidly. The response of CK-BB in blood was rapider and more sensitive than that of CK-MB or CK-MM. The CK-BB concentrations were significantly higher in coronary sinus samples than in arterial samples. These results suggest that the major portion of elevated blood CK-BB level in the early phase after reperfusion are derived from the heart muscle.
Usui
A
A
Department of Thoracic Surgery, Nagoya University School of Medicine, Japan.
Kato
K
K
Abe
T
T
Murase
M
M
Tanaka
M
M
Takeuchi
E
E
D002315:Cardiopulmonary Bypass; D003402:Creatine Kinase / Q000097:blood* / Q000652:urine; D006350:Heart Valve Prosthesis; D006801:Humans; D007124:Immunoenzyme Techniques; D007432:Intraoperative Period; D007527:Isoenzymes; D008943:Mitral Valve / Q000601:surgery; D015425:Myocardial Reperfusion
D007527:Isoenzymes; D003402:Creatine Kinase