[The role of gonadotropins, cyclic AMP, 22-R-hydroxycholesterol and cofactors in regulating endocrine functions of the Leydig cells in rats. I. Effect of cofactors on the synthesis of steroid hormones in the interstitial gland of rats].
The authors evaluated the influence of NAD, NADP, G-6-P and G-6-PDH on the synthesis of steroid hormones in the interstitial gland of the rat. The animals were killed by decapitation, and sections of the nucleus weighing altogether 20 mg were incubated with NAD (0.4 mM), NADP (0.4 mM), G-6-P (3.5 mM) and G-6-PDH (2 j.m./ml) for 4 hours in CO2 incubator. Then the tissue was homogenized, removed by centrifugation and then from the homogenous supernatant the authors extracted steroids which were in the incubating medium and in the tissue. Steroid hormones examined were marked radioimmunologically, they were: pregnenolon as the first and testosterone as the second stage of synthesis of nucleus androgens. The search carried out shows that the strongest stimulator in biosynthesis of pregnenolon was NADP together with the reducing system (G-6-P and G-6-PDH). On the other hand, in case of testosterone the highest effectiveness was achieved by simultaneous use of the cofactors examined. Applying cofactors separately the authors were able to find that both NAD and NADP together with the reducing system were responsible for testosterone synthesis and their simultaneous applying in the incubating medium leads to a synergistic effect.
Grochowski
D
D
Szamatowicz
M
M
D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D004357:Drug Synergism; D019298:Glucose-6-Phosphate; D005954:Glucosephosphate Dehydrogenase / Q000494:pharmacology*; D005958:Glucosephosphates / Q000494:pharmacology*; D066298:In Vitro Techniques; D007985:Leydig Cells / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009243:NAD / Q000494:pharmacology*; D009249:NADP / Q000494:pharmacology*; D011284:Pregnenolone / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D013268:Stimulation, Chemical; D013739:Testosterone / Q000096:biosynthesis*
D003470:Culture Media; D005958:Glucosephosphates; D009243:NAD; D013739:Testosterone; D009249:NADP; D019298:Glucose-6-Phosphate; D011284:Pregnenolone; D005954:Glucosephosphate Dehydrogenase
Rola gonadotropin, cAMP, 22-R-hydroksycholesterolu i kofaktorów w regulacji funkcji endokrynnej komórek Leydiga u szczura. I. Wpływ kofaktorów na synteze hormonów sterydowych w gruczole śródmiazszowym szczura.