Spontaneous and luminol-dependent chemiluminescences from tissue preparations of benzo[a]pyrene-injected mice.
Chemiluminescence (CL) from tissue preparations of mice i.p. injected with and without the chemical carcinogen benzo[a]pyrene (BP) was detected by a single photon counting apparatus. The spontaneously emitted CL (spontaneous CL) and the CL after luminol addition (luminol-CL) were measured for mice with and without previous induction of their liver mixed-function oxidases (MFO) by phenobarbital. In MFO-non-induced mice, although the spontaneous CL was not notably modified by BP injection, the kidneys presented three times greater luminol-CL after BP injection. On the other hand, MFO-induced mice had higher spontaneous CL of plasma, liver, kidneys, and lungs, as well as higher luminol-CL of liver after BP injection, when compared with the respective MFO-induced but BP-non-injected mice. The luminol-CL of liver was suppressed by scavengers of active oxygen and free-radicals such as TIRON, butylhydroxytoluene and in a less extent by superoxide dismutase. The CL detected from tissue preparations of mice after BP treatment is thought to reflect the formation of oxygen radicals and electronically excited-species during BP metabolism.
Yoshida
L S
LS
Department of Food Chemistry, Tohoku University, Sendai, Japan.
Miyazawa
T
T
Fujimoto
K
K
Kaneda
T
T
D014013:1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt / Q000494:pharmacology; D000818:Animals; D001564:Benzo(a)pyrene / Q000378:metabolism / Q000494:pharmacology*; D001769:Blood; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D005609:Free Radicals; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D008163:Luminescent Measurements; D008165:Luminol / Q000494:pharmacology*; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D006899:Mixed Function Oxygenases / Q000096:biosynthesis; D010634:Phenobarbital / Q000494:pharmacology; D011724:Pyridazines / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000494:pharmacology
D005609:Free Radicals; D011724:Pyridazines; D002084:Butylated Hydroxytoluene; D001564:Benzo(a)pyrene; D014013:1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt; D008165:Luminol; D006899:Mixed Function Oxygenases; D013482:Superoxide Dismutase; D010634:Phenobarbital