title: Epidermal growth factor excretion and receptor binding in diabetic rats.
abstract: Urinary epidermal growth factor (EGF) excretion was calculated as ng EGF per mg creatinine and ng EGF per 24 hr. It was increased 4-9 fold in rats with genetic (BB) or streptozotocin-induced diabetes. It decreased to 2-3 fold control values in insulin-treated animals. In contrast, EGF concentration in serum was lower in diabetic than in control rats (360 +/- 72 vs 524 +/- 150 pg/ml, P .086); EGF level in plasma was unchanged (319 +/- 67 vs 313 +/- 96 pg/ml). In diabetic rats EGF content was increased in submaxillary glands (1018 +/- 259 vs 738 +/- 122 pg/mg protein, P .060) but unchanged in the kidneys (70 +/- 18 vs 65 +/- 6 pg/mg protein in controls). EGF binding to the liver microsomes in diabetic rats was decreased by 30-40% and was not restored by insulin therapy. Binding to the kidneys also showed a tendency to decrease in diabetic animals. The EGF excretion and receptor binding were normal in obese normoglycemic Zucker fa/fa rats. We suggest that hyperglycemia and/or glucosuria may affect EGF synthesis and/or excretion in the kidneys and EGF synthesis or accumulation in the megakaryocytes. The mechanism of decreased EGF receptor binding remains to be clarified.
authors.affiliation: Department of Diabetes, Endocrinology and Metabolism City of Hope National Medical Center, Duarte, California.
authors.forename: D L
authors.initials: DL
authors.lastname: Hwang
authors.forename: A
authors.initials: A
authors.lastname: Lev-Ran
authors.forename: Y C
authors.initials: YC
authors.lastname: Tay
authors.forename: C R
authors.initials: CR
authors.lastname: Chen
authors.forename: N
authors.initials: N
authors.lastname: Dev
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000097:blood / Q000378:metabolism / Q000652:urine*; D066246:ErbB Receptors / Q000378:metabolism*; D007328:Insulin / Q000627:therapeutic use; D007668:Kidney / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D051381:Rats; D011913:Rats, Inbred BB; D011919:Rats, Inbred Strains; D011924:Rats, Zucker; D013363:Submandibular Gland / Q000378:metabolism
chemical_list: D007328:Insulin; D004815:Epidermal Growth Factor; D066246:ErbB Receptors