title: NK sensitivity, H-2 expression and metastatic potential: analysis of H-2Dk gene transfected fibrosarcoma cells.
abstract: We have used the 3-Methylcholanthrene induced T-10 fibrosarcoma tumour cell system (H-2b xH-2k)F1 to elucidate the possible correlation between metastatic potential, expression of individual H-2 antigens and susceptibility to NK cells. Transfection of the non-metastatic and NK sensitive IC9 cells (Db+, Dk-, Kb-, Kk-) with the H-2Dk gene, altered the metastatic phenotype of the parental cells, yet had no effect on the susceptibility of these tumour cells to lysis by NK and did not elicit a specific CTL response in syngeneic hosts. Variants of the metastatic and NK resistant IE7 clone (Db+, Dk+, Kb-, Kk-), lacking H-2Dk, were selected by treatment with monoclonal anti H-2Dk antibodies and complement. These variants were sensitive to NK and poorly or non metastatic. Retransfection of 'Dk' 'loss' variants with the H-2Dk gene, resulted in the isolation of several clones expressing a wide range of metastatic phenotypes but maintained sensitivity to NK. These results indicate that the H-2D region of the MHC and or closely linked genes may be involved in the complex interrelationship between target susceptibility to NK and metastasis.
authors.affiliation: Department of Microbiology & Immunology, Faculty of Health Sciences, Ben Gurion University, Beer Sheva, Israel.
authors.forename: J
authors.initials: J
authors.lastname: Gopas
authors.forename: B
authors.initials: B
authors.lastname: Rager-Zisman
authors.forename: I
authors.initials: I
authors.lastname: Har-Vardi
authors.forename: G J
authors.initials: GJ
authors.lastname: Hammerling
authors.forename: M
authors.initials: M
authors.lastname: Bar-Eli
authors.forename: S
authors.initials: S
authors.lastname: Segal
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D003165:Complement System Proteins; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D005354:Fibrosarcoma / Q000139:chemically induced / Q000235:genetics / Q000276:immunology* / Q000556:secondary; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology*; D064532:Histocompatibility Antigen H-2D; D007694:Killer Cells, Natural / Q000276:immunology*; D008748:Methylcholanthrene; D051379:Mice; D008807:Mice, Inbred BALB C; D010641:Phenotype; D014162:Transfection
chemical_list: D000911:Antibodies, Monoclonal; D006183:H-2 Antigens; D064532:Histocompatibility Antigen H-2D; D008748:Methylcholanthrene; D003165:Complement System Proteins