A syngeneic monoclonal anti-idiotype antibody against HNK-1: characterization and cytotoxic activity in vitro.
A syngeneic, monoclonal, anti-idiotype antibody, G6D9, was raised against the mouse monoclonal IgM HNK-1. G6D9, characterized as an IgG1-kappa, inhibits the binding of HNK-1 to the myelin-associated glycoprotein (MAG). G6D9 does not interfere with the binding of various human monoclonal anti-MAG IgM to their specific antigen. G6D9 binds HNK-1 hybridoma cells on their surface and within their cytoplasm, as demonstrated using indirect immunofluorescence. In the presence of complement, G6D9 is cytotoxic for HNK-1-secreting cells. A cell lysis of 32% was observed and compared to lysis obtained with other antibodies directed against mouse lymphocyte antigens. The use of G6D9 as a tool to study specific cytotoxicity and immunotherapy in vivo is discussed.
Schluep
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Department of Neurology, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.
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D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D003165:Complement System Proteins / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D007075:Immunoglobulin M; D003165:Complement System Proteins