Chemotactic effect of PAF-acether on peritoneal eosinophils from normal rats.
The chemotactic activity of PAF-acether was compared with that of tetrapeptide eosinophil chemotactic factors of anaphylaxis (ECF-A, Ala-Gly-Ser-Glu and Val-Gly-Ser-Glu) using eosinophils obtained from the peritoneal cavity of normal rats. Cells were isolated by separation over discontinuous metrizamide gradients which resulted in eosinophil suspensions of 80 to 90% purity. PAF-acether produced a dose-dependent effect which, at its maximum, was more than 30-fold greater than control and about 5- to 7-fold greater than the maximal activity obtained with the ECF-A-tetrapeptides. BN 52021 and WEB 2086 inhibited PAF-acether-induced eosinophil chemotaxis in a dose-dependent manner, suggesting that this phenomenon is mediated by specific PAF-acether receptors.
Martins
M A
MA
Departamento de Fisiologia e Farmacodinâmica, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
Etienne
A
A
Soulard
C
C
Domingo
M T
MT
Braquet
P
P
D000818:Animals; D001381:Azepines / Q000494:pharmacology; D002630:Chemotactic Factors / Q000494:pharmacology*; D002631:Chemotactic Factors, Eosinophil / Q000494:pharmacology*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D004224:Diterpenes; D004804:Eosinophils / Q000502:physiology*; D046934:Ginkgolides; D066298:In Vitro Techniques; D007783:Lactones / Q000494:pharmacology; D008297:Male; D010529:Peritoneal Cavity / Q000166:cytology*; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D014230:Triazoles / Q000494:pharmacology
D001381:Azepines; D002630:Chemotactic Factors; D002631:Chemotactic Factors, Eosinophil; D004224:Diterpenes; D046934:Ginkgolides; D007783:Lactones; D010972:Platelet Activating Factor; D014230:Triazoles; C052518:WEB 2086; C045856:ginkgolide B