title: Experimental trichinellosis in horses: biological and parasitological evaluation.
abstract: Three groups of three horses each were, respectively, infected with 5000, 20,000 and 50,000 larvae of Trichinella spiralis. The strain used was isolated from a human biopsy during horsemeat-related outbreaks of trichinellosis in France. Transient muscular disorders were only observed in two of the horses infected with 50,000 larvae but none of the horses had fever. A significant increase in blood eosinophils was noticed in 5 horses. Serum LDH, aldolase and CPK peaked at the fifth week post-infection. Specific IgG assayed by indirect immunofluorescence and ELISA, appeared 2-5 weeks post-infection and disappeared between 16 and 40 weeks. The distribution of T. spiralis larvae was maximal in the tongue, masseters and diaphragm, but a large decrease in the number of larvae recovered from the muscles was noticed among the horses slaughtered at the beginning and end of the experiment. In muscular histological sections, larvae were observed in an intramyofibrillar position and were surrounded by a mild to severe inflammatory reaction.
authors.affiliation: Laboratoire Central de Recherches Vétérinaires, Maisons-Alfort, France.
authors.forename: C
authors.initials: C
authors.lastname: Soule
authors.forename: J
authors.initials: J
authors.lastname: Dupouy-Camet
authors.forename: P
authors.initials: P
authors.lastname: Georges
authors.forename: T
authors.initials: T
authors.lastname: Ancelle
authors.forename: J P
authors.initials: JP
authors.lastname: Gillet
authors.forename: J
authors.initials: J
authors.lastname: Vaissaire
authors.forename: A
authors.initials: A
authors.lastname: Delvigne
authors.forename: E
authors.initials: E
authors.lastname: Plateau
mesh_terms: D000818:Animals; D003402:Creatine Kinase / Q000097:blood; D003964:Diaphragm / Q000469:parasitology; D004797:Enzyme-Linked Immunosorbent Assay; D004804:Eosinophils; D005260:Female; D005455:Fluorescent Antibody Technique; D005634:Fructose-Bisphosphate Aldolase / Q000097:blood; D006734:Horse Diseases / Q000097:blood / Q000276:immunology / Q000469:parasitology*; D006736:Horses; D007074:Immunoglobulin G / Q000032:analysis; D007770:L-Lactate Dehydrogenase / Q000097:blood; D007958:Leukocyte Count / Q000662:veterinary; D008297:Male; D008406:Masseter Muscle / Q000469:parasitology; D014059:Tongue / Q000469:parasitology; D014235:Trichinellosis / Q000097:blood / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*
chemical_list: D007074:Immunoglobulin G; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase; D005634:Fructose-Bisphosphate Aldolase