Temperature-sensitive sec mutants of Escherichia coli: inhibition of protein export at the permissive temperature.
Phenotypes of secY and secA temperature-sensitive mutants at permissive (low) temperature have been examined. The secY24 mutant was found to be extremely susceptible to export inhibition by a basal-level synthesis of the MalE-LacZ 72-47 hybrid protein or to overproduction of a normal secretory protein such as maltose-binding protein or beta-lactamase. Comparison of this phenotype of secY24 with those of the secY100 and secA51 mutants under similar conditions suggested that MalE-LacZ protein and overproduced secretory protein do not nonspecifically enhance the partial secretion defect but act synergistically with secY24 to inhibit protein export.
Ito
K
K
Institute for Virus Research, Kyoto University, Japan.
Hirota
Y
Y
Akiyama
Y
Y
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D010641:Phenotype; D011499:Protein Processing, Post-Translational; D013696:Temperature
D001425:Bacterial Outer Membrane Proteins