Construction of chickpea genomic library.
For construction of chickpea genomic library, DNA was isolated, purified on CsCl gradient and size fractionated into 15-20 Kb fragments after restriction with Sau 3A. These fragments were ligated to phage lambda (EMBL-3) vector and the recombinant molecules packaged in vitro into viable phage particles. The recombinant phages were obtained as phages on a P2 lysogen of E. coli (Spi- selection) and amplified to establish a permanent library. This is the first report of the construction of chickpea genomic library.
Koundal
K R
KR
Mehta
S L
SL
Sharma
R P
RP
D004247:DNA / Q000302:isolation & purification; D007887:Fabaceae / Q000235:genetics*; D015698:Genomic Library; D010946:Plants, Medicinal
D004247:DNA