title: Granulocyte-macrophage colony-stimulating factor (CSF) and multilineage CSF recruit human monocytes to express granulocyte CSF.
abstract: We assessed the capacity of recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF) and multilineage (Multi)-CSF to induce release of granulocyte-CSF (G-CSF) by highly purified peripheral blood monocyte (Mo) preparations. Our results reveal that GM-CSF and Multi-CSF, either alone or in a synergistic concert, activate Mo to transcribe G-CSF messenger (m) RNA and release biologically active G-CSF protein into their culture supernatants. G-CSF had no regulatory effect on Mo expression of cytoplasmic G-CSF mRNA levels and G-CSF protein secretion by itself. These differential actions of CSFs provide further insight into self-regulatory mechanisms within the growth factor hierarchy system.
authors.affiliation: Department of Hematology, Johannes Gutenberg University, Mainz, FRG.
authors.forename: W
authors.initials: W
authors.lastname: Oster
authors.forename: A
authors.initials: A
authors.lastname: Lindemann
authors.forename: R
authors.initials: R
authors.lastname: Mertelsmann
authors.forename: F
authors.initials: F
authors.lastname: Herrmann
mesh_terms: D002474:Cell-Free System; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000494:pharmacology* / Q000502:physiology; D004338:Drug Combinations; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006098:Granulocytes / Q000502:physiology*; D006133:Growth Substances / Q000494:pharmacology* / Q000502:physiology; D006801:Humans; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D012333:RNA, Messenger / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D003115:Colony-Stimulating Factors; D004338:Drug Combinations; D006133:Growth Substances; D012333:RNA, Messenger; D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor