Restoration of prostaglandin E2-producing splenic macrophages in 89Sr-treated mice with bone marrow from Corynebacterium parvum primed donors.
Administration of Corynebacterium parvum (CP), 56 mg/kg ip to CBA/J mice effected the induction of prostaglandin E2 (PGE2) producing macrophages (M phi) in the bone marrow and the spleen. Maximal release of PGE2 from M phi cultured in vitro with calcium ionophore A23187 for 2 h was reached by marrow M phi removed on 5 days after CP (450 ng/mg cell protein), and by splenic M phi 9 days after CP (400 ng/mg). Neither M phi population, however, yielded more than 6.0 ng/mg leukotriene C4. To assess ontogenic relationships mice were depleted of bone marrow and blood monocytes by iv injection of the bone-seeking isotope, 89Sr. CP was given at several points before or after bone marrow cell depletion. PGE2 production by splenic M phi harvested on day 9 after CP was profoundly impaired when CP was administered either concurrently with or 3 days after 89Sr. When CP was administered 1, 3, 5, and 7 days before 89Sr, however, the induction of PGE2-producing M phi in the spleen was unaffected. To determine whether bone marrow cells from CP-injected donors can restore PGE2-producing splenic M phi (PGSM) in 89Sr-mice, recipient mice which had and had not received CP 3 days after 89Sr were transfused with 5 x 10(6) syngeneic bone marrow cells from donor mice prepared at varying intervals after CP administration. The results clearly indicate the capacity of bone marrow cells harvested on either day 1 or 2 following CP to restore PGSM in CP-primed, but not unprimed, recipients.(ABSTRACT TRUNCATED AT 250 WORDS)
Shibata
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Department of Pathology, East Carolina University School of Medicine, Greenville, NC 27858-4353.
D000818:Animals; D001853:Bone Marrow / Q000276:immunology* / Q000528:radiation effects; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation / Q000276:immunology; D003352:Corynebacterium / Q000276:immunology*; D005260:Female; D007700:Kinetics; D008264:Macrophages / Q000276:immunology / Q000378:metabolism* / Q000528:radiation effects; D051379:Mice; D008808:Mice, Inbred CBA; D009000:Monocytes / Q000276:immunology; D011458:Prostaglandins E / Q000096:biosynthesis*; D013154:Spleen / Q000166:cytology / Q000276:immunology* / Q000528:radiation effects; D013156:Splenectomy; D013326:Strontium Radioisotopes / Q000008:administration & dosage
D011458:Prostaglandins E; D013326:Strontium Radioisotopes