A method for the efficient transfer of isolated mitochondria into yeast protoplasts.
When protoplasts isolated from non-respiring rho0 strains of S. cerevisiae are incubated with mitochondria from respiring rho+ strains in the presence of polyethylene glycol, a small fraction of the acceptor protoplasts acquires respiratory capacity. Pretreatment of the isolated mitochondria and protoplasts with Ca2+ ions enhanced the frequency of ensuing respiration-competent colonies by almost three orders of magnitude. When mitochondria were isolated from a strain exhibiting killer capacity as a cytoplasmic marker, in addition to the appropriate nuclear and mitochondrial markers, about one-third of the ensuing rho+ colonies were not killers. Possible mechanisms for the transfer of mitochondria into acceptor protoplasts were studied.
Sulo
P
P
Institute of Animal Physiology, Slovak Academy of Sciences, Ivanka pri Dunaji, Czechoslovakia.
Griac
P
P
Klobucníková
V
V
Kovác
L
L
D005819:Genetic Markers; D008928:Mitochondria / Q000378:metabolism / Q000502:physiology*; D010101:Oxygen Consumption; D010641:Phenotype; D011523:Protoplasts / Q000378:metabolism / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000648:ultrastructure*
D005819:Genetic Markers