title: Transient expression of the proto-oncogene int-1 during differentiation of P19 embryonal carcinoma cells.
abstract: In mouse embryos, the int-1 proto-oncogene is transiently expressed in areas of the developing neural system. Retinoic acid-treated P19 embryonal carcinoma cells have often been used as an in vitro model for the molecular basis of neural development. We shown here that int-1 is transiently expressed in differentiated P19 cells. The time course and retinoic acid dose dependence of int-1 expression suggest that the gene is specifically expressed during early neural differentiation. P19 cells may be a useful model to assist in the study, at the cellular level, of the role of int-1 in neural development.
authors.affiliation: Division of Molecular Biology, Netherlands Cancer Institute (Antoni van Leeuwenhoekhuis), Amsterdam.
authors.forename: E
authors.initials: E
authors.lastname: Schuuring
authors.forename: L
authors.initials: L
authors.lastname: van Deemter
authors.forename: H
authors.initials: H
authors.lastname: Roelink
authors.forename: R
authors.initials: R
authors.lastname: Nusse
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D009474:Neurons / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000235:genetics; D011519:Proto-Oncogenes / Q000187:drug effects; D013724:Teratoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D051153:Wnt Proteins; D051155:Wnt1 Protein; D029961:Zebrafish Proteins
chemical_list: D011518:Proto-Oncogene Proteins; D051153:Wnt Proteins; D051155:Wnt1 Protein; D029961:Zebrafish Proteins; D014212:Tretinoin