Fused transcripts of c-myc and a new cellular locus, hcr in a primary liver tumor.
The proto-oncogene c-myc has been implicated in the formation of primary liver tumors in hepatitis virus-infected woodchucks. In one of these tumors, a DNA rearrangement placed the truncated c-myc gene downstream of a cellular sequence (hcr) in a head-to-tail configuration resulting in 50-fold enhanced levels of c-myc transcripts. Analysis of the tumor-specific c-myc RNA now demonstrates that transformed liver cells produce fused hcr/myc transcripts initiated from the hcr promoter and extending into c-myc coding sequences by differential splicing mechanisms. In phase fusion of the reading frames of both genes might result in the translation of the hcr/myc 2.0 kb RNA into a hybrid protein that would differ from the normal woodchuck c-myc gene product by 22 additional hcr amino acids at its amino-terminus. The production of inappropriate levels of modified or normal myc-encoded proteins is probably involved in the malignant process.
Etiemble
J
J
Unité de Rëcombinaison et Expression Génétique, INSERM U.163, CNRS UA.271, Institut Pasteur, Paris, France.
Möröy
T
T
Jacquemin
E
E
Tiollais
P
P
Buendia
M A
MA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA; D015342:DNA Probes; D005786:Gene Expression Regulation; D015321:Gene Rearrangement; D008114:Liver Neoplasms, Experimental / Q000235:genetics*; D008392:Marmota; D008969:Molecular Sequence Data; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis; D016271:Proto-Oncogene Proteins c-myc; D011519:Proto-Oncogenes; D012334:RNA, Neoplasm / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D015183:Restriction Mapping
D015342:DNA Probes; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D012334:RNA, Neoplasm; D011993:Recombinant Fusion Proteins; D004247:DNA