Detection and interspecies-transformation of a beta-lactamase-encoding plasmid from Pasteurella haemolytica.
Pasteurella haemolytica-cultures, isolated from cattle with respiratory diseases, were investigated for their biotype, serotype, antimicrobial resistance and plasmid content. A plasmid encoding a beta-lactamase could be demonstrated in 9 of 19 Pasteurella haemolytica-cultures. These 9 cultures, all belonging to biotype A and serotype 1, were resistant to ampicillin, carbenicillin, penicillin G and ticarcillin. The plasmid of the respective cultures proved to be identical upon Southern blot hybridization. It could be transformed into Escherichia coli 490 A where it expressed again a beta-lactamase-activity.
Schwarz
S
S
Institut für Bakteriologie und Immunologie, Justus-Liebig-Universität Giessen.
Spies
U
U
Reitz
B
B
Seyfert
H M
HM
Lämmler
C
C
Blobel
H
H
D000668:Ampicillin Resistance / Q000235:genetics; D000818:Animals; D001345:Autoradiography; D015373:Bacterial Typing Techniques; D015139:Blotting, Southern; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D004269:DNA, Bacterial / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D009693:Nucleic Acid Hybridization; D010325:Pasteurella / Q000145:classification / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D010326:Pasteurella Infections / Q000382:microbiology / Q000662:veterinary; D010403:Penicillin Resistance / Q000235:genetics*; D011815:R Factors; D012141:Respiratory Tract Infections / Q000382:microbiology / Q000662:veterinary; D012703:Serotyping; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology; D014169:Transformation, Bacterial; D001618:beta-Lactamases / Q000235:genetics*
D004269:DNA, Bacterial; D001618:beta-Lactamases