Identification and characterization of polypeptide growth factors secreted by murine embryonal carcinoma cells.
Undifferentiated P19 and PC13 murine embryonal carcinoma (EC) cells have been analyzed for their ability to secrete polypeptide growth factors. This has been carried out by a combination of specific bioassays and the use of biochemical and immunological detection methods. Both P19 and PC13 EC cells secrete a platelet-derived growth factor (PDGF)-like growth factor, a type beta transforming growth factor, and insulin-like growth factors. In addition, PC13 EC cells secrete a heparin-binding growth factor functionally related to fibroblast growth factor, while P19 EC cells secrete transforming growth factor-alpha. This is the first demonstration for secretion of transforming growth factor-alpha by an equivalent of early embryonic cells. The possible paracrine growth stimulating effects of these growth factors have been tested on differentiated derivatives of P19 EC cells, corresponding to all three germ layers. The differences in growth factor production by various embryonal carcinoma cells are discussed in relation to the developmental origin of these cell lines.
van Zoelen
E J
EJ
Hubrecht laboratory, Netherlands Institute for Developmental Biology, Utrecht.
Ward-van Oostwaard
T M
TM
Nieuwland
R
R
van der Burg
B
B
van den Eijnden-van Raaij
A J
AJ
Mummery
C L
CL
De Laat
S W
SW
D000818:Animals; D001681:Biological Assay; D002455:Cell Division; D002851:Chromatography, High Pressure Liquid; D054278:Embryonal Carcinoma Stem Cells; D005346:Fibroblast Growth Factors / Q000378:metabolism; D006133:Growth Substances / Q000378:metabolism* / Q000494:pharmacology; D006493:Heparin / Q000378:metabolism; D015151:Immunoblotting; D007163:Immunosorbent Techniques; D051379:Mice; D008970:Molecular Weight; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D010982:Platelet-Derived Growth Factor / Q000378:metabolism; D013002:Somatomedins / Q000378:metabolism; D015291:Transforming Growth Factors / Q000378:metabolism; D014407:Tumor Cells, Cultured
D006133:Growth Substances; D010982:Platelet-Derived Growth Factor; D013002:Somatomedins; D005346:Fibroblast Growth Factors; D015291:Transforming Growth Factors; D006493:Heparin