Immunological evidence that insulin activates protein kinase C in BC3H-1 myocytes.
Effects of insulin on immunoreactive protein kinase C were examined in BC3H-1 myocytes. Insulin provoked rapid dose-dependent decreases in cytosolic enzyme, and transient increases and subsequent decreases in membrane-associated enzyme. Phorbol esters provoked similar changes. Our findings suggest that insulin provokes the translocative activation of protein kinase C.
Acevedo-Duncan
M
M
James A. Haley Veterans' Hospital, Research Service, Tampa, FL 33612.
Cooper
D R
DR
Standaert
M L
ML
Farese
R V
RV
D015153:Blotting, Western; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology; D003600:Cytosol / Q000201:enzymology; D004789:Enzyme Activation / Q000187:drug effects*; D007328:Insulin / Q000494:pharmacology*; D009132:Muscles / Q000201:enzymology*; D011493:Protein Kinase C / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D007328:Insulin; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate