Estimate of Plasmodium falciparum sporozoite content of Anopheles stephensi used to challenge human volunteers.
Plasmodium falciparum infected Anopheles stephensi, taken from a group of mosquitoes which had been used to challenge recipients of (NANP)3-TT vaccine, were tested for P. falciparum sporozoite content by an immunoradiometric assay. Seventy-six percent were infected with mean and median sporozoite equivalents per mosquito of 220,994 and 217,398, respectively (SD = 54,911). This sporozoite density is greater than that usually found in the field. These data suggest that this challenge for evaluating P. falciparum sporozoite vaccines is a demanding test of immunity.
Davis
J R
JR
Department of Medicine, University of Maryland School of Medicine, Baltimore.
Murphy
J R
JR
Clyde
D F
DF
Baqar
S
S
Cochrane
A H
AH
Zavala
F
F
Nussenzweig
R S
RS
D000818:Animals; D000852:Anopheles / Q000469:parasitology*; D000953:Antigens, Protozoan / Q000032:analysis; D002986:Clinical Trials as Topic / Q000379:methods; D006801:Humans; D008288:Malaria / Q000517:prevention & control; D010963:Plasmodium falciparum / Q000276:immunology* / Q000302:isolation & purification; D014612:Vaccines / Q000276:immunology
D000953:Antigens, Protozoan; D014612:Vaccines