title: [Aspergillus fumigatus: evaluations of the standardization of diagnostic extracts].
abstract: Extracts of Aspergillus fumigatus used for serodiagnosis of allergic diseases are of unknown and nonstandardized composition. Applying immunoprint technique subsequent to isoelectric focusing commercial and self-prepared extracts are compared. By use of an ABPA-pool serum and monoclonal antibodies recognizing antigens of this fungus, different immunological reactivities of the extracts can be demonstrated. It is suggested to use monoclonal antibodies as secondary standards as well as to improve standardization.
authors.affiliation: Medizinische Klinik des Forschungsinstituts Borstel.
authors.forename: J S
authors.initials: JS
authors.lastname: Trompelt
authors.forename: W M
authors.initials: WM
authors.lastname: Becker
authors.forename: M
authors.initials: M
authors.lastname: Schlaak
mesh_terms: D000911:Antibodies, Monoclonal / Q000592:standards; D000946:Antigens, Fungal / Q000276:immunology* / Q000592:standards; D001232:Aspergillus fumigatus / Q000276:immunology*; D005656:Fungal Proteins / Q000276:immunology* / Q000592:standards; D007525:Isoelectric Focusing
chemical_list: D000911:Antibodies, Monoclonal; D000946:Antigens, Fungal; D005656:Fungal Proteins
vernacular_title: Aspergillus fumigatus: Ansätze zur Standardisierung von Extrakten für die Diagnostik.