title: Antigen-induced lung solute clearance in rats is dependent on capsaicin-sensitive nerves.
abstract: Chemosensitive sensory nerves have an important effector role in the control of vascular permeability in rat airways after neurogenic inflammation. To investigate whether they also have a role in antigen-induced lung inflammation, we have studied the changes in lung solute clearance (LSC) in sensitized rats after aerosol challenge with allergen and the effect of prior capsaicin-induced denervation on these changes. Sprague-Dawley rats were immunized with egg albumin (EA), using aluminum hydroxide and Bordetella pertussis as adjuvants. After 11 days, the animals were challenged for 5 min with aerosolized EA, and the clearance from the lungs of aerosolized 99mTc diethylenetriamine pentaacetic acid (99mTc-DTPA) over 7.5 min (LSC 7.5) was subsequently measured at various times after challenge as an index of epithelial permeability or integrity. Sensitized animals responded to the challenge with immediate respiratory symptoms and with an increased 99mTc-DTPA clearance rate that was detectable at 20 min (mean +/- SE LSC 7.5: baseline, 6 +/- 1%; 20 min, 17 +/- 3%; p less than 0.05), persisted at 4 h (14 +/- 1%; p less than 0.05), and returned to normal values after 24 h. Unsensitized rats exposed to EA and sensitized rats exposed to PBS or to bovine serum albumin did not show any change. Bronchoalveolar lavage failed to show significant changes of cell populations until 24 h, when an increased presence of lymphocytes, PMN, and eosinophils was observed.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Pathology, McMaster University, Hamilton, Ontario, Canada.
authors.forename: P
authors.initials: P
authors.lastname: Sestini
authors.forename: M
authors.initials: M
authors.lastname: Dolovich
authors.forename: C
authors.initials: C
authors.lastname: Vancheri
authors.forename: R H
authors.initials: RH
authors.lastname: Stead
authors.forename: J S
authors.initials: JS
authors.lastname: Marshall
authors.forename: M
authors.initials: M
authors.lastname: Perdue
authors.forename: J
authors.initials: J
authors.lastname: Gauldie
authors.forename: J
authors.initials: J
authors.lastname: Bienenstock
mesh_terms: D000336:Aerosols; D000485:Allergens / Q000276:immunology; D000818:Animals; D000941:Antigens / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002211:Capsaicin / Q000494:pharmacology*; D002628:Chemoreceptor Cells / Q000187:drug effects* / Q000276:immunology; D003714:Denervation; D005260:Female; D007114:Immunization / Q000379:methods; D008168:Lung / Q000000981:diagnostic imaging / Q000187:drug effects / Q000276:immunology* / Q000294:innervation; D008297:Male; D009942:Organometallic Compounds; D004369:Pentetic Acid; D011247:Pregnancy; D011877:Radionuclide Imaging; D051381:Rats; D011919:Rats, Inbred Strains; D016284:Technetium Tc 99m Pentetate; D013997:Time Factors
chemical_list: D000336:Aerosols; D000485:Allergens; D000941:Antigens; D009942:Organometallic Compounds; D004369:Pentetic Acid; D002211:Capsaicin; D016284:Technetium Tc 99m Pentetate