[Intracellular localization and tissue specificity of human uracil-DNA glycosylase].
Uracil-DNA glycosylase (UDG) purified by various procedures from the human placenta was used to obtain immune antisera with specific antibodies, the antibodies being affinity-purified on UDG-sepharose. Two immunoreactive polypeptides were found in crude extracts of the human placenta with the help of the antibodies. Their apparent molecular masses were about 37,000 and 34,000 dalton. Only the former polypeptide was found in crude extracts of the human embryonal heart, liver and in HeLa cells. The indirect immunofluorescent staining shows both slight and intensive fluorescence of HeLa cell nuclei. The similarity of antigenic properties of the human and rat UDG was confirmed.
Kuz'min
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Mikhaĭlov
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D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D000918:Antibody Specificity; D045647:DNA Glycosylases; D004622:Embryo, Mammalian; D005260:Female; D006367:HeLa Cells; D006801:Humans; D007158:Immunologic Techniques; D008099:Liver / Q000201:enzymology; D008970:Molecular Weight; D009206:Myocardium / Q000201:enzymology; D009699:N-Glycosyl Hydrolases / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D009928:Organ Specificity; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy; D051381:Rats; D051981:Uracil-DNA Glycosidase
D000906:Antibodies; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D051981:Uracil-DNA Glycosidase
Vnutrikletochnaia lokalizatsiia i tkanespetsifichnost' uratsil-DNK glikozilazy cheloveka.