[In vitro experimental expansion and induction of LAK cell of lymphocytes from human carcinomatous ascites].
Investigation on the in vitro expansion and induction of LAK cell activity of peritoneal exudate lymphocytes (PEL) from malignant ascites of advanced ovarian cancer patients is presented. The results indicated that to isolate PEL from malignant ascites was much more simple and economic than to isolate tumor infiltrating lymphocytes (TIL). PEL could be in vitro expanded 17-fold and induced to acquire LAK cell activity in the presence of rIL-2. Thus, PEL can be used as LAK cell precursor for adoptive immunotherapy of cancer.
Li
D J
DJ
Cancer Institute, Haerbin University of Medical Sciences, Haerbin.
D000230:Adenocarcinoma / Q000276:immunology; D001202:Ascitic Fluid / Q000276:immunology; D002455:Cell Division; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology*; D004915:Leukemia, Erythroblastic, Acute / Q000276:immunology; D008175:Lung Neoplasms / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D010051:Ovarian Neoplasms / Q000276:immunology*; D014407:Tumor Cells, Cultured
D007376:Interleukin-2