Some factors inducing formation of metacyclic stages of Trypanosoma cruzi.
Epimastigotes of Trypanosoma cruzi, Peru strain, incubated in Contreras' artificial triatomine urine transformed into metacyclic trypomastigotes within 48 h at 28 degrees C when 10 mM L-proline or L-glutamate was added to the medium. Smaller numbers of metacyclic stages were induced in the presence of glucose or L-alanine. The L-leucine and L-isoleucine, 2 amino acids known to inhibit proline catabolism, inhibited proline-induced metacyclogenesis. Cells gassed with 5% CO2 showed significantly faster rates and higher levels of transformation than those not gassed, therefore indicating the additional importance of CO2 for transformation.
Homsy
J J
JJ
Department of Developmental and Cell Biology, University of California, Irvine 92717.
Granger
B
B
Krassner
S M
SM
D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D005947:Glucose / Q000494:pharmacology; D005971:Glutamates / Q000494:pharmacology; D007523:Isocitrates / Q000494:pharmacology; D007532:Isoleucine / Q000494:pharmacology; D007656:Ketoglutaric Acids / Q000494:pharmacology; D007930:Leucine / Q000494:pharmacology; D009952:Ornithine / Q000494:pharmacology; D010071:Oxaloacetates / Q000494:pharmacology; D011392:Proline / Q000494:pharmacology; D014349:Trypanosoma cruzi / Q000187:drug effects / Q000254:growth & development*
D000596:Amino Acids; D005971:Glutamates; D007523:Isocitrates; D007656:Ketoglutaric Acids; D010071:Oxaloacetates; D007532:Isoleucine; D011392:Proline; C034219:isocitric acid; D009952:Ornithine; D007930:Leucine; D005947:Glucose