title: Purification, properties, and developmental changes of cellular retinol-binding protein, type II, in chicken intestine.
abstract: Two distinct cellular retinol-binding proteins were detected in chicken small intestine. A predominant form was purified to homogeneity. The apparent molecular weight of this protein was estimated to be 17,200. This form was larger than a second minor form partially purified (molecular weight of 15,000). The absorption and fluorescence spectra of the bound retinol to the purified proteins were typical for the known cellular retinol-binding proteins. The results suggest that the purified binding protein corresponds to CRBP(II), previously identified in small intestine of rats and humans. To gain an insight into the possible role of CRBP(II) in chicken small intestine, the CRBP(II) contents in cytosols of small intestine of embryonic and post-hatch chicks were determined by enzyme-linked immunosorbent assay. The amount of CRBP(II) per unit DNA in small intestine was low at 15- and 17-day embryonic stage, but rapidly increased around the period of hatching. The increased level was still maintained in 6-week-old chicks, which accounted for 0.9% of total proteins in duodenum. The developmental pattern and the presence of abundant amount of CRBP(II) in chicken small intestine supports the hypothesis that CRBP(II) might play some role in the intestinal absorption of retinoids. Thus the involvement of a tissue-specific cellular retinol-binding protein in the intestinal absorption of retinoids appears to be common in mammalian and avian species.
authors.affiliation: Laboratory of Nutritional Physiology, School of Food and Nutritional Sciences, University of Shizuoka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Goda
authors.forename: S
authors.initials: S
authors.lastname: Takase
mesh_terms: D000818:Animals; D002642:Chick Embryo; D002645:Chickens / Q000378:metabolism*; D002845:Chromatography; D004386:Duodenum / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D007082:Ileum / Q000378:metabolism; D007421:Intestine, Small / Q000032:analysis / Q000196:embryology / Q000254:growth & development* / Q000378:metabolism; D007583:Jejunum / Q000378:metabolism; D008970:Molecular Weight; D012177:Retinol-Binding Proteins / Q000302:isolation & purification* / Q000378:metabolism; D054840:Retinol-Binding Proteins, Cellular; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry
chemical_list: D012177:Retinol-Binding Proteins; D054840:Retinol-Binding Proteins, Cellular