title: Large-scale purification of human islets utilizing discontinuous albumin gradient on IBM 2991 cell separator.
abstract: A new method is described for the large-scale purification of human pancreatic islets with a discontinuous gradient of bovine serum albumin formed on an IBM 2991 cell separator. Fifteen human pancreases were processed, and after density-gradient centrifugation, a mean of 2643 islets/ml pancreatic digest were recovered with a mean purity of 63% and contained in 430 microliter mean vol. Viability of gradient-isolated islets was compared with that of non-density-gradient islets (handpicked) and showed no difference in function. This technique allows isolation of intact, viable human islets of Langerhans of sufficient purity for potential human transplantation.
authors.affiliation: Department of Surgery, University of Leicester, England, United Kingdom.
authors.forename: S P
authors.initials: SP
authors.lastname: Lake
authors.forename: P D
authors.initials: PD
authors.lastname: Bassett
authors.forename: A
authors.initials: A
authors.lastname: Larkins
authors.forename: J
authors.initials: J
authors.lastname: Revell
authors.forename: K
authors.initials: K
authors.lastname: Walczak
authors.forename: J
authors.initials: J
authors.lastname: Chamberlain
authors.forename: G M
authors.initials: GM
authors.lastname: Rumford
authors.forename: N J
authors.initials: NJ
authors.lastname: London
authors.forename: P S
authors.initials: PS
authors.lastname: Veitch
authors.forename: P R
authors.initials: PR
authors.lastname: Bell
mesh_terms: D000328:Adult; D000818:Animals; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000601:surgery; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology*; D051381:Rats; D011923:Rats, Nude