Translocation of c-abl oncogene and PDGFB (c-sis) gene in a case of CML with 46,XY, t(22;22).
In a case of CML with a variant Philadelphia translocation (Ph1 or Ph) t(22;22) (q11;q13) in bone marrow cells and unstimulated peripheral blood cells, no cytogenetically detectable involvement of chromosome 9 was observed. Southern blot experiments using probes specific for bcr and c-sis however revealed rearrangement of the bcr, but not of PDGFB (c-sis) gene. Northern blot analysis of bone marrow RNA showed a very weak signal with the c-sis probe, while in a lymph-node biopsy PDGFB m-RNA could not be detected. Chromosomal in situ hybridization gave evidence for translocation of c-abl from chromosome 9 to Ph and of PDGFB from chromosome 22 to chromosome 9, as the result of a threefold translocation t(9;22;22).
Gradl
G
G
Institut für Humangenetik, Medizinische Universität zu Lübeck, Federal Republic of Germany.
Tesch
H
H
Schwieder
G
G
Wagner
T
T
Fonatsch
C
C
D001853:Bone Marrow / Q000032:analysis; D002892:Chromosomes, Human, Pair 22; D002899:Chromosomes, Human, Pair 9; D004247:DNA / Q000032:analysis; D006133:Growth Substances / Q000235:genetics; D006801:Humans; D007621:Karyotyping; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D008198:Lymph Nodes / Q000032:analysis; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D010677:Philadelphia Chromosome; D010982:Platelet-Derived Growth Factor / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016315:Proto-Oncogene Proteins c-abl; D020574:Proto-Oncogene Proteins c-sis; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000032:analysis; D014178:Translocation, Genetic
D006133:Growth Substances; D010982:Platelet-Derived Growth Factor; D011518:Proto-Oncogene Proteins; D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger; D004247:DNA; D016315:Proto-Oncogene Proteins c-abl