The effect of nonreceptor adsorption on the lethal action of colicin E1.
The survivability of Escherichia coli K12s cells has been studied after treatment with 125I-labeled colicin E1. It has been shown that for low amounts of adsorbed colicin the survivability follows single-hit kinetics. When the number of colicin molecules adsorbed exceeds approx. 50 per cell, deviation from single-hit kinetics occurs towards higher survivability. Colicin E1 adsorbed nonreceptorwise by the cell's surface has been shown to inhibit the lethal action of colicin E1 molecules adsorbed at specific receptors. This fact has been used in accounting for the elevated survivability of cells at high colicin doses. The functional significance of the phenomenon is discussed.
Malkhosyan
S R
SR
Institute of Molecular Genetics, USSR Academy of Sciences, Moscow.
Rekesh
A N
AN
D000327:Adsorption; D002462:Cell Membrane / Q000187:drug effects; D002463:Cell Membrane Permeability / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects*; D003087:Colicins / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism; D007700:Kinetics
D003087:Colicins