Simple method for quantitation of cell-bound protein A on Staphylococcus aureus cells by means of hemagglutination with sheep erythrocytes differentially sensitized with rabbit antibody and its clinical application.
A simple method for quantitation of cell-bound protein A (SpA) on organisms of Staphylococcus aureus was successfully devised by using hemagglutination between staphylococcal organisms and a series of sheep erythrocyte suspensions sensitized with different amounts of anti sheep erythrocyte rabbit antiserum. The validity of the principle and the reproducibility of the method presented here were precisely analyzed and the details of the method are presented. The hemagglutination was quantitatively inhibited both by normal rabbit serum and by soluble SpA. Using the method presented here, 376 strains of S. aureus freshly isolated from clinical materials were subjected to SpA quantitation in cell-bound form. According to the results, there was an unexpected distribution profile in the amounts of cell-bound SpA among the clinical isolates, which showed a two-peak pattern. A possible usefulness of the method presented here in clinical investigations is briefly discussed also.
Hwang
S M
SM
Department of Clinical Pathology, Jisan Junior College, Pusan, Korea.
Seki
K
K
Murai
M
M
Sakurada
J
J
Nishihara
S
S
Maeda
T
T
Masuda
S
S
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001431:Bacteriological Techniques; D004912:Erythrocytes / Q000276:immunology; D006385:Hemagglutination Inhibition Tests; D007114:Immunization; D011817:Rabbits; D012756:Sheep; D013205:Staphylococcal Protein A / Q000032:analysis* / Q000276:immunology; D013211:Staphylococcus aureus / Q000032:analysis* / Q000276:immunology
D000942:Antigens, Bacterial; D013205:Staphylococcal Protein A