N myristylation of the human immunodeficiency virus type 1 gag polyprotein precursor in Saccharomyces cerevisiae.
A semisynthetic gene precisely encoding the 502 amino acids of the human immunodeficiency virus type 1 gag precursor (Pr53gag) was expressed in the yeast Saccharomyces cerevisiae. Amino acid sequence analysis of the recombinant Pr53gag showed that the amino terminus was fully blocked. Labeling of Pr53gag with [3H]myristic acid demonstrated that, as with Pr53gag isolated from virus-infected cells, the yeast-derived protein was demethionylated and N myristylated on glycine, the second amino acid residue.
Bathurst
I C
IC
Chiron Corporation, Emeryville, California 94608-2916.
Chester
N
N
Gibson
H L
HL
Dennis
A F
AF
Steimer
K S
KS
Barr
P J
PJ
D015683:Gene Products, gag; D005796:Genes; D005813:Genes, Synthetic; D005814:Genes, Viral; D005822:Genetic Vectors; D015497:HIV-1 / Q000201:enzymology / Q000235:genetics*; D019814:Myristic Acid; D009227:Myristic Acids / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D015183:Restriction Mapping; D012191:Retroviridae Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D015683:Gene Products, gag; D009227:Myristic Acids; D012191:Retroviridae Proteins; D019814:Myristic Acid; D011505:Protein-Tyrosine Kinases