[Determination of saponosides from Panax ginseng C.A. Meyer. Comparison of official methods and HPLC].
Official methods for determination of ginsenosides of the French and Helvetic Pharmacopoeias have been compared with HPLC method. Sample preparation schemes used are those of monographs with conventional solvent extraction and solid phase extraction with a polar and a non-polar sorbents, respectively kieselguhr and C 18 octadecyl. Liquid-solid sample clean-up with C 18 cartridge is the most effective procedure. Prior HPLC method, an hydrolysis step of malonylginsenosides is necessary. Very selective extraction resulting in highly purified solution authorizes reliable and rapid colorimetric determination from ginseng saponosides.
Guédon
D
D
Abbe
P
P
Cappelaere
N
N
Rames
N
N
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003124:Colorimetry; D005894:Panax / Q000032:analysis*; D010946:Plants, Medicinal / Q000032:analysis; D012503:Saponins / Q000302:isolation & purification*
D012503:Saponins
Dosage des saponosides de Panax ginseng C.A. Meyer. Comparison entre les méthodes officielles et la CLHP.