Functional expression of chicken calmodulin in yeast.
The coding region of a chicken calmodulin cDNA was fused to a galactose-inducible GAL1 promoter, and an expression system was constructed in the yeast Saccharomyces cerevisiae. Expression of calmodulin was demonstrated by purifying the heterologously expressed protein and analyzing its biochemical properties. When the expression plasmid was introduced into a calmodulin gene (cmd1)-disrupted strain of yeast, the cells grew in galactose medium, showing that chicken calmodulin could complement the lesion of yeast calmodulin functionally. Repression of chicken calmodulin in the (cmd1)-disrupted strain caused cell cycle arrest with a G2/M nucleus, as observed previously with a conditional-lethal mutant of yeast calmodulin. These results suggest that the essential function of calmodulin for cell proliferation is conserved in cells ranging from yeast to vertebrate cells.
Ohya
Y
Y
Department of Biology, Faculty of Science, University of Tokyo, Japan.
Anraku
Y
Y
D000818:Animals; D002147:Calmodulin / Q000235:genetics*; D002453:Cell Cycle; D002645:Chickens / Q000235:genetics*; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D005816:Genetic Complementation Test; D008970:Molecular Weight; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013045:Species Specificity
D002147:Calmodulin