[Formation of mixed colonies by progenitor cells of chronic myeloid leukemia cultured in vivo].
The clonal growth of multipotential progenitor cells of chronic myeloid leukemia (CML) in diffusion chamber implanted into peritoneal cavity of neutropenic and anemic rats was assessed. CML precursors formed in methylcellulose culture enriched with medium conditioned by phytohemagglutinin activated lymphocytes mixed neutrophilic-erythroid colonies, in number significantly higher then normal cells. Mixed colonies produced by CML cells, in contrast to the normal precursors, contained also macrophages, eosinophils and megakaryocytes. We conclude that modified diffusion chamber culture technique may be a suitable tool for multipotential progenitor study in CML.
Hansz
J
J
Sawiński
K
K
Woźny
T
T
D000328:Adult; D000818:Animals; D002452:Cell Count; D002455:Cell Division / Q000502:physiology; D015194:Diffusion Chambers, Culture; D015672:Erythroid Precursor Cells / Q000473:pathology*; D005260:Female; D006098:Granulocytes / Q000473:pathology*; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D014411:Neoplastic Stem Cells / Q000473:pathology*; D010529:Peritoneal Cavity; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014410:Tumor Stem Cell Assay
Wytwarzanie kolonii mieszanych przez komórki macierzyste przewlekłej białaczki szpikowej w hodowli in vivo.