[Establishment of a cell line (HCe-8693) from human undifferentiated adenocarcinoma of the cecum and its characterization].
A human cecum undifferentiated adenocarcinoma cell line (HCe-8693) was established from a metastatic lymph node of the surgical specimen from a male adult patient. The cell doubling time was 30.4 hr and the mitotic index was 28.8%. All the cells were of epithelial type, round and oval in shape. Electron microscopic examination showed large nucleus, clear-cut nucleoli, abundant microvilli and some secretory granules. Chromosomal analysis revealed a mode of 48 per cell. The frequency of colony formation in soft agar was 8%. The cells agglutinated in 1 microgram/ml PHA or ConA. CEA was positive in the cells and in the culture supernatant. When hetero-transplanted to nude mice, HCe-8693 cells grew to form tumor with the same morphology as the original one from the patient with Alcian blue positive material in the cytoplasm. Repeated detection for mycoplasma was negative.
Zhang
Z X
ZX
Cancer Research Institute, Zhejiang Cancer Hospital, Hangzhou.
D000230:Adenocarcinoma / Q000473:pathology* / Q000648:ultrastructure; D000818:Animals; D002272:Carcinoembryonic Antigen / Q000378:metabolism; D002430:Cecal Neoplasms / Q000473:pathology* / Q000648:ultrastructure; D002869:Chromosome Aberrations; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014407:Tumor Cells, Cultured
D002272:Carcinoembryonic Antigen