title: Detection of essential arginine in bacterial peptidyl dipeptidase-4: arginine is not the anion binding site.
abstract: Peptidyl dipeptidase-4 from Pseudomonas maltophilia was modified with the arginine reagents p-hydroxyphenylglyoxal and 2,3-butanedione. The enzyme was inactivated in a pseudo-first-order manner by p-hydroxyphenylglyoxal with a half-time of 72 min. Inactivation by 2,3-butanedione was biphasic with a rapid phase followed by a slower inactivation to less than 10% activity within 24h. The competitive inhibitor thiorphan protected against inactivation by phydroxyphenylglyoxal and by 2,3-butanedione also but to a lesser degree. Inhibitory anions chloride and phosphate did not protect against inactivation by either reagent. These data support the conclusion that an active site arginine is essential for substrate hydrolysis. Furthermore, arginine is not the binding site for the inhibitors chloride and phosphate.
authors.affiliation: New England Medical Center Hospital, Department of Medicine, Boston, MA 02111.
authors.forename: J J
authors.initials: JJ
authors.lastname: Lanzillo
authors.forename: Y
authors.initials: Y
authors.lastname: Dasarathy
authors.forename: B L
authors.initials: BL
authors.lastname: Fanburg
mesh_terms: D000838:Anions; D001120:Arginine; D001665:Binding Sites; D002712:Chlorides / Q000378:metabolism* / Q000494:pharmacology; D003931:Diacetyl / Q000494:pharmacology; D010450:Endopeptidases / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D010658:Phenylglyoxal / Q000031:analogs & derivatives / Q000494:pharmacology; D010710:Phosphates / Q000378:metabolism* / Q000494:pharmacology; D011480:Protease Inhibitors; D011549:Pseudomonas / Q000201:enzymology*; D013379:Substrate Specificity; D015244:Thiorphan / Q000494:pharmacology
chemical_list: D000838:Anions; D002712:Chlorides; D010710:Phosphates; D011480:Protease Inhibitors; C028465:4-hydroxyphenylglyoxal; D001120:Arginine; D015244:Thiorphan; D010450:Endopeptidases; C047929:peptidyl dipeptidase 4; D003931:Diacetyl; D010658:Phenylglyoxal