In vivo toxicity of cisplatin and carboplatin on the Leydig cell function and effect of the human choriogonadotropin.
In order to characterize the respective toxicity of cisplatin (CDDP) and carboplatin (CBDCA) on the male reproductive system, we have investigated their in vivo effects on the steroidogenesis function of rat leydig cells. Animals were treated at the respective LD50 of platin compounds, and we analyzed plasmatic testosterone level, microsomal cytochrome P-450 and platinum concentrations in the testis. CDDP induces a dramatic change in both the testosterone level and the microsomal cytochrome P-450 concentration. In contrast, CBDCA was found to be less toxic than CDDP, probably due to its different accumulation at the testis level. We also investigated the potential action of human chorionic gonadotropin which allows a full restoration of the steroidogenesis function.
Azouri
H
H
Laboratoire de Toxicologie, Faculté des Sciences Pharmaceutiques, Chatenay-Malabry, France.
Bidart
J M
JM
Bohuon
C
C
D000818:Animals; D016190:Carboplatin; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D002945:Cisplatin / Q000633:toxicity*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D007985:Leydig Cells / Q000187:drug effects*; D008297:Male; D008861:Microsomes / Q000201:enzymology; D009944:Organoplatinum Compounds / Q000633:toxicity*; D010984:Platinum / Q000493:pharmacokinetics; D051381:Rats; D011919:Rats, Inbred Strains; D013739:Testosterone / Q000097:blood
D006063:Chorionic Gonadotropin; D009944:Organoplatinum Compounds; D013739:Testosterone; D010984:Platinum; D003577:Cytochrome P-450 Enzyme System; D016190:Carboplatin; D002945:Cisplatin