HLA class I gene expression on human primary tumours and autologous metastases: demonstration of selective losses of HLA antigens on colorectal, gastric and laryngeal carcinomas.
The expression of HLA class I antigens was studied in 99 primary tumour (colorectal, gastric and laryngeal carcinomas) and 57 autologous metastases using immunohistological techniques and monoclonal antibodies against class I monomorphic determinants, HLA-B isotypic determinants and HLA polymorphic determinants. Fourteen per cent of colorectal, 9.6% of gastric and 20% of laryngeal carcinomas completely lacked class I molecules. Selective losses of HLA-B antigens were also detected in 8.8, 3.4 and 5.8% of these tumours respectively. Taking into account complete and selective loss of HLA-B the average alteration in the class I molecules expression totalled 21%. The comparison of class I expression between primary tumours and autologous metastases showed differences in 24% of the patients. These differences consisted mainly in a decrease of class I expression by metastases. Nevertheless, four types of divergence were detected in laryngeal carcinomas, namely: +/-, +/+, -/+, -/-. In addition, a clear correlation between degree of differentiation and class I expression was observed in laryngeal tumours. Finally, when class I gene RFLPs were compared with DNA from 15 tumours and autologous normal mucosa or peripheral lymphocytes, no differences were detected between these samples.
López-Nevot
M A
MA
Servicio de Análisis Clínicos, Hospital Virgen de las Nieves, Granada, Spain.
Esteban
F
F
Ferrón
A
A
Gutiérrez
J
J
Oliva
M R
MR
Romero
C
C
Huelin
C
C
Ruiz-Cabello
F
F
Garrido
F
F
D015139:Blotting, Southern; D015179:Colorectal Neoplasms / Q000235:genetics / Q000276:immunology*; D005805:Genes, MHC Class I; D006680:HLA Antigens / Q000032:analysis*; D015235:HLA-B Antigens / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D007822:Laryngeal Neoplasms / Q000235:genetics / Q000276:immunology*; D008207:Lymphatic Metastasis; D013274:Stomach Neoplasms / Q000235:genetics / Q000276:immunology*
D006680:HLA Antigens; D015235:HLA-B Antigens