title: Cellular forms of the rat and human beta-amyloid precursor protein (BAPP).
abstract: Using synthetic peptide antisera generated against beta-amyloid precursor protein (BAPP) sequences we demonstrate that the newly synthesized BAPP appears as a 94 kDa peptide in rat brain RNA cell-free translates or polysome runoffs, while from similar human brain material we see two bands at 94 and 120 kDa. Immunoblots reveal likely post-translational modification of BAPP, and possibly an age-dependent, endogenous proteolysis of the protein in rat brain. On human brain immunoblots an unusual post-translational modification or possibly oligomerization of BAPP is seen, particularly in cortical regions.
authors.affiliation: Fishberg Center for Neurobiology, Mount Sinai School of Medicine, New York, NY 10029.
authors.forename: J
authors.initials: J
authors.lastname: Anderson
authors.forename: W
authors.initials: W
authors.lastname: Wallace
authors.forename: S
authors.initials: S
authors.lastname: Snyder
authors.forename: V
authors.initials: V
authors.lastname: Haroutunian
authors.forename: J L
authors.initials: JL
authors.lastname: Roberts
authors.forename: I
authors.initials: I
authors.lastname: Lieberburg
mesh_terms: D000375:Aging / Q000378:metabolism*; D000682:Amyloid / Q000378:metabolism*; D016229:Amyloid beta-Peptides; D000818:Animals; D001921:Brain / Q000378:metabolism*; D006801:Humans; D007106:Immune Sera; D008970:Molecular Weight; D011498:Protein Precursors / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D051381:Rats; D011916:Rats, Inbred F344; D011919:Rats, Inbred Strains
chemical_list: D000682:Amyloid; D016229:Amyloid beta-Peptides; D007106:Immune Sera; D011498:Protein Precursors