Glucose-stimulated efflux of fura-2 in pancreatic beta-cells is prevented by probenecid.
Fura-2 loaded pancreatic beta-cells, isolated from obese hyperglycemic mice, were studied with respect to cytoplasmic free Ca2+ concentration ([Ca2+]i), insulin release and efflux of indicator. In the absence of glucose there was a continuous efflux of fura-2, which was markedly increased by stimulation with a high concentration of the sugar. Probenecid both reduced basal efflux of fura-2 and prevented that promoted by glucose. There was no interference of the drug with glucose-induced either insulin release or rise in [Ca2+]i. When applying fura-2 in pancreatic beta-cells, the use of probenecid markedly improves the measurements of [Ca2+]i.
Arkhammar
P
P
Department of Medical Cell Biology, University of Uppsala, Sweden.
Nilsson
T
T
Berggren
P O
PO
D000818:Animals; D001572:Benzofurans / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D005456:Fluorescent Dyes; D016257:Fura-2; D005947:Glucose / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051379:Mice; D008820:Mice, Obese; D011339:Probenecid / Q000494:pharmacology*
D001572:Benzofurans; D005456:Fluorescent Dyes; D007328:Insulin; D005947:Glucose; D011339:Probenecid; D002118:Calcium; D016257:Fura-2