[Analysis of chloramphenicol in milk and animal tissues using the gas chromatography method].
Chloramphenicol is extracted from meat, liver, kidney and milk samples with ethyl acetate, followed by hexane partitioning with aqueous sodium chloride to remove lipids, ethyl acetate reextraction, formation of trimethylsilyl derivative of chloramphenicol and quantitation by electron capture gas chromatography. Precision, accuracy and recovery of the method was tested. The average recoveries from meat samples spiked at 25, 50 and 100 micrograms/kg levels ranged from 94.7 to 98.7% with coefficient of variation (CV) in the range 3.6-8.3%. Recovery and CV values from milk samples spiked at three levels ranged from 90.4 to 97.2% and from 4.9 to 6.6%, respectively. The detection limit of the method was 5 micrograms/kg.
Niewiadowska
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Semeniuk
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D000818:Animals; D002849:Chromatography, Gas / Q000379:methods; D005506:Food Contamination / Q000032:analysis*; D007668:Kidney / Q000032:analysis; D008099:Liver / Q000032:analysis; D008452:Maximum Allowable Concentration; D008460:Meat / Q000032:analysis* / Q000633:toxicity; D008892:Milk / Q000032:analysis* / Q000633:toxicity; D009132:Muscles / Q000032:analysis; D011044:Poland; D013552:Swine
Oznaczanie chloramfenikolu w mleku i tkankach zwierzat metoda chromatografii gazowej.