title: Dissecting tumor cell invasion: epithelial cells acquire invasive properties after the loss of uvomorulin-mediated cell-cell adhesion.
abstract: The generation of invasiveness in transformed cells represents an essential step of tumor progression. We show here, first, that nontransformed Madin-Darby canine kidney (MDCK) epithelial cells acquire invasive properties when intercellular adhesion is specifically inhibited by the addition of antibodies against the cell adhesion molecule uvomorulin; the separated cells then invade collagen gels and embryonal heart tissue. Second, MDCK cells transformed with Harvey and Moloney sarcoma viruses are constitutively invasive, and they were found not to express uvomorulin at their cell surface. These data suggest that the loss of adhesive function of uvomorulin (which is identical to E-cadherin and homologous to L-CAM) is a critical step in the promotion of epithelial cells to a more malignant, i.e., invasive, phenotype. Similar modulation of intercellular adhesion might also occur during invasion of carcinoma cells in vivo.
authors.affiliation: Friedrich-Miescher-Laboratorium der Max-Planck-Gesellschaft, Tübingen, Federal Republic of Germany.
authors.forename: J
authors.initials: J
authors.lastname: Behrens
authors.forename: M M
authors.initials: MM
authors.lastname: Mareel
authors.forename: F M
authors.initials: FM
authors.lastname: Van Roy
authors.forename: W
authors.initials: W
authors.lastname: Birchmeier
mesh_terms: D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D000954:Antigens, Surface / Q000502:physiology*; D015820:Cadherins; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules; D002460:Cell Line; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D003094:Collagen; D004848:Epithelium / Q000473:pathology; D005455:Fluorescent Antibody Technique; D066298:In Vitro Techniques; D008562:Membrane Glycoproteins / Q000502:physiology*; D008854:Microscopy, Electron; D009206:Myocardium / Q000166:cytology; D009362:Neoplasm Metastasis; D009374:Neoplasms, Experimental / Q000473:pathology*
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D015820:Cadherins; D015815:Cell Adhesion Molecules; D008562:Membrane Glycoproteins; D003094:Collagen