Recognition of insulin on MHC-class-II-expressing L929 cells by antibody and T cells.
In the present experiments, we attempted to obtain evidence that T-cell receptors (Tcr) and immunoglobulins can react against the same antigen-peptide/MHC class II complexes on antigen-presenting cells (APC). Use was made of monoclonal APC, i.e. I-Ak alpha/beta gene-transfected L929 fibroblasts, monoclonal anti-insulin antibodies and selected insulin-specific T-cell lines. Evidence for similarities in antibody and T-cell recognition of insulin presented by I-Ak alpha/beta gene-transfected L-cell fibroblasts was not obtained after serious attempts. Furthermore, we found no evidence for synthesis of antibodies specific for insulin-peptide/MHC class II complexes.
Rubin
B
B
Institute for Experimental Immunology, University of Copenhagen, Denmark.
Malissen
B
B
Jørgensen
P N
PN
Zeuthen
J
J
D000818:Animals; D000911:Antibodies, Monoclonal; D000938:Antigen-Presenting Cells / Q000276:immunology; D000949:Histocompatibility Antigens Class II; D007328:Insulin / Q000276:immunology* / Q000378:metabolism; D007330:Insulin Antibodies; D007739:L Cells; D051379:Mice; D008815:Mice, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II; D007328:Insulin; D007330:Insulin Antibodies