Partial purification and characterization of a new p36/40 tyrosine protein kinase from HL-60.
A major peak of tyrosine protein kinase activity was partially purified from a Triton X100 extract of HL-60. This preparation submitted to high pressure gel filtration was eluted at a volume corresponding to a mass of 35/40 kD. This activity was insensitive to EGF and insulin. Autoradiographs of the preparations incubated with [gamma P32]-ATP and separated by electrophoresis do not give any evidence that autophosphorylation occurs for that particular tyrosine protein kinase. Furthermore, we failed to immunoprecipitate the enzyme with a specific antiphosphotyrosine antibody and anti v-src antibody. All the data presented herein suggest that this enzyme has not been previously purified.
Boutin
J A
JA
Division Pathologies Cancereuses et Immunes, Institut de Recherches SERVIER, Suresnes, France.
Ernould
A P
AP
Genton
A
A
Cudennec
C A
CA
D000255:Adenosine Triphosphate / Q000378:metabolism; D001345:Autoradiography; D001667:Binding, Competitive; D002851:Chromatography, High Pressure Liquid; D004587:Electrophoresis, Agar Gel; D004815:Epidermal Growth Factor / Q000494:pharmacology; D006160:Guanosine Triphosphate / Q000378:metabolism; D006801:Humans; D007163:Immunosorbent Techniques; D007328:Insulin / Q000494:pharmacology; D015473:Leukemia, Promyelocytic, Acute / Q000201:enzymology*; D008970:Molecular Weight; D017830:Octoxynol; D010766:Phosphorylation; D011092:Polyethylene Glycols; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000378:metabolism; D013379:Substrate Specificity; D014407:Tumor Cells, Cultured
D007328:Insulin; D011092:Polyethylene Glycols; D004815:Epidermal Growth Factor; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D017830:Octoxynol; D011505:Protein-Tyrosine Kinases