Detection of dystrophin on two-dimensional gel electrophoresis.
A protein with MW approximately 350 k daltons and pI approximately 5.5, which was deleted in the dystrophic mouse (C57BL/10ScSn-mdx), was detected on two-dimensional gel electrophoresis with silver staining. Deletion of this protein was uniformly observed in the dystrophic mouse extensor digitus longus, soleus and cardiac muscle. This protein specifically reacted with the monoclonal antibody against the chemically synthesized N-terminal fragment of human dystrophin. The protein reacting with this monoclonal antibody was also detected in rabbit back-muscle, rat extensor digitus longus and human skeletal muscle at the same position as the mouse muscle protein, on the two-dimensional gel electrophoresis. Our results show that dystrophin is solubilized in 8M guanidine HCl and that the modified two-dimensional gel electrophoresis can be applied to separate dystrophin.
Hori
S
S
Department of Neurochemistry, Tokyo Metropolitan Institute for Neurosciences, Japan.
Sugiura
H
H
Shimizu
T
T
Hirabayashi
T
T
Ohtani
S
S
Yoshida
M
M
Miyamoto
K
K
Tanabe
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D016189:Dystrophin; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D015151:Immunoblotting; D007526:Isoelectric Point; D051379:Mice; D009124:Muscle Proteins / Q000032:analysis* / Q000276:immunology; D009137:Muscular Dystrophy, Animal / Q000503:physiopathology; D011817:Rabbits; D051381:Rats
D000911:Antibodies, Monoclonal; D016189:Dystrophin; D009124:Muscle Proteins