Export of prepro-alpha-factor from Escherichia coli.
Yeast prepro-alpha-factor translocates posttranslationally into yeast microsomes in vitro. This process is strongly influenced by the extreme carboxyl-terminal region of the protein. These features contrast with the properties of most eucaryotic proteins which are translocated into the endoplasmic reticulum. We have extended these studies by introducing the gene for the wild-type and several mutant forms of prepro-alpha-factor into Escherichia coli. Prepro-alpha-factor is secreted into the periplasm and processed to pro-alpha-factor. Its translocation across the plasma membrane requires the membrane potential and the secY gene product. Deletion mutant analysis showed that features of the pro-segment were essential for secretion of prepro-alpha-factor in E. coli, while the carboxyl-terminal region, which is required in yeast, is dispensible in E. coli. Neither size nor the presence of a unique topogenic sequence was sufficient to explain the requirement for the pro-segment.
Lecker
S
S
Molecular Biology Institute, University of California, Los Angeles 90024-1737.
Meyer
D
D
Wickner
W
W
D002872:Chromosome Deletion; D004926:Escherichia coli / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins
D005656:Fungal Proteins; C048632:MF(ALPHA)1 protein, S cerevisiae; D011498:Protein Precursors; D029701:Saccharomyces cerevisiae Proteins