title: Comparison of whole-blood cyclosporine levels measured by radioimmunoassay and fluorescence polarization in patients post orthotopic liver transplant.
abstract: This study compared the analysis of whole blood cyclosporine concentrations measured by fluorescence polarization immunoassay (FPIA) and radioimmunoassay (RIA) polyclonal and monoclonal procedures. Fifteen orthotopic liver transplant patients with a mean age of 39 +/- 11.06 years were included in the study. One hundred thirteen levels were analyzed using FPIA, RIA polyclonal, and RIA monoclonal procedures. There was no difference statistically in comparing FPIA and RIA polyclonal results (p greater than 0.05). There was a statistical difference between FPIA and RIA monoclonal results p = 0.0001). With use of least squares simple linear regression analysis, FPIA results showed good correlation with RIA polyclonal results (R2 = 0.87). Poor correlation was shown between FPIA and RIA monoclonal results (R2 = 0.51). In this study population, FPIA produced results 2.5% higher than the RIA polyclonal procedure.
authors.affiliation: Department of Surgery, Emory University School of Medicine, Atlanta, Georgia.
authors.forename: M A
authors.initials: MA
authors.lastname: Hooks
authors.forename: W J
authors.initials: WJ
authors.lastname: Millikan
authors.forename: J M
authors.initials: JM
authors.lastname: Henderson
authors.forename: R
authors.initials: R
authors.lastname: Mullins
authors.forename: V
authors.initials: V
authors.lastname: Lampasona
mesh_terms: D000328:Adult; D003524:Cyclosporins / Q000097:blood* / Q000493:pharmacokinetics; D005260:Female; D005454:Fluorescence Polarization; D006801:Humans; D016031:Liver Transplantation; D008297:Male; D008875:Middle Aged; D011863:Radioimmunoassay; D014184:Transplantation, Homologous
chemical_list: D003524:Cyclosporins