[Isolation of the porcine alpha-interferon gene using simultaneous directed multipoint mutagenesis].
A method of obtaining the pig alpha-interferon gene by means of simultaneous multidirected mutagenesis of the human alpha 2-interferon gene is presented. Nucleotide homology between these genes is 80.4%. Fourteen synthetic oligonucleotides forming a pig alpha-interferon gene's strand were ligated on a single-stranded template, carrying cDNA of the human alpha 2-interferon gene. The obtained DNA fragment was cloned in the single-stranded or double-stranded form. It was found that the method does not affect the cloning efficiency. The primary structure of the gene was confirmed by sequencing.
Chernov
I P
IP
Rostapshov
V M
VM
Azhikina
T L
TL
Borodin
A M
AM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005813:Genes, Synthetic; D006801:Humans; D007370:Interferon Type I / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D013552:Swine
D007370:Interferon Type I; D004247:DNA
Poluchenie gena alpha-interferona svin'i metodom odnovremennogo napravlennogo mnogotochechnogo mutageneza.