Fate of conceptus and corpus luteum after induced embryonic loss in heifers.
Ultrasonography was performed to determine the fate of the conceptus and the corpus luteum after induced embryonic loss in heifers. When embryonic loss was induced by administration of prostaglandin F2 alpha (25 mg, IM) on day 28 (n = 10) or day 42 (n = 10), embryonic death occurred 2.3 +/- 0.2 (mean +/- SEM) and 2.6 +/- 0.2 days, respectively, after treatment, and subsequent ovulation occurred 2.4 +/- 0.3 and 2.7 +/- 0.2 days, respectively, after embryonic death. Administration of a mitotic inhibitor (1 mg of colchicine, intrauterine; n = 4) on day 42 resulted in embryonic death in 1.2 +/- 0.2 days and ovulation in 20.8 +/- 3.1 days after embryonic death. Rupture of the amnion on day 42 (n = 3) resulted in immediate death of the embryo and ovulation in 35 +/- 3.5 days. In prostaglandin-treated heifers, area of the corpus luteum decreased (P less than 0.01) between days -2 and -1, relative to day of embryonic death. Volume of uterine contents remained approximately constant for 2 or 3 days after treatment, then decreased greater than 75% between examinations on consecutive days. Embryonic death apparently resulted from luteolysis and was characterized by rapid loss of the conceptus with minimal conceptus degeneration; an intact conceptus was recovered from the vagina of 3 heifers treated on day 42. In heifers treated with colchicine or in which the amnion was ruptured, embryonic death was associated with maintenance of the corpus luteum and prolonged retention of the conceptus, which underwent extensive degeneration.(ABSTRACT TRUNCATED AT 250 WORDS)
Kastelic
J P
JP
Department of Veterinary Science, University of Wisconsin, Madison 53706.
Ginther
O J
OJ
D000028:Abortion, Induced / Q000662:veterinary; D000034:Abortion, Veterinary / Q000503:physiopathology*; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000503:physiopathology*; D003078:Colchicine / Q000008:administration & dosage; D003338:Corpus Luteum / Q000503:physiopathology*; D015237:Dinoprost / Q000008:administration & dosage; D005260:Female; D005313:Fetal Death / Q000662:veterinary; D011247:Pregnancy; D014463:Ultrasonography / Q000662:veterinary*
D015237:Dinoprost; D003078:Colchicine