title: In situ pyrimidine dimer determination by laser cytometry.
abstract: By using antiserum against pyrimidine dimers and argon-laser imaging microspectrofluorometry, we established a new method to determine UV-induced pyrimidine dimers and their repair in individual human cells. The method was sensitive enough to determine dimers induced by UV dose as low as 2 J/m2. Normal human cells repaired 50 and 60% of total damage within 8 and 24 h after UV irradiation (20 J/m2), but Xeroderma pigmentosum cells (complementation group A) were unable to repair any within the same period. Therefore, the method proved to be a quick, easy, sensitive and accurate means to determine pyrimidine dimers in situ.
authors.forename: T
authors.initials: T
authors.lastname: Mori
authors.forename: A A
authors.initials: AA
authors.lastname: Wani
authors.forename: S M
authors.initials: SM
authors.lastname: D'Ambrosio
authors.forename: C C
authors.initials: CC
authors.lastname: Chang
authors.forename: J E
authors.initials: JE
authors.lastname: Trosko
mesh_terms: D004260:DNA Repair; D005455:Fluorescent Antibody Technique; D006801:Humans; D007834:Lasers; D011740:Pyrimidine Dimers / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D014466:Ultraviolet Rays
chemical_list: D011740:Pyrimidine Dimers