Structural comparison of the Pichia pastoris alcohol oxidase genes.
In methylotrophic yeasts, alcohol oxidase is the first enzyme in the methanol-utilization pathway. The genome of one such yeast, Pichia pastoris, contains two alcohol oxidase genes, AOX1 and AOX2. Sequence analysis indicated that each gene encodes a similar protein of 663 amino acids. The protein-coding regions of the genes were 92% and 97% homologous at the nucleotide and predicted amino acid sequence levels, respectively. In contrast to homology observed within the protein-coding portions of the AOX genes, no homology was found in either the 5' or 3' non-coding regions. Although alcohol oxidase is found in peroxisomes of P. pastoris, the AOX amino acid sequences did not contain a peptide sequence similar to the peroxisomal transport sequence found at the C-terminus of some peroxisomally located proteins in higher eukaryotes.
Koutz
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Salk Institute Biotechnology/Industrial Associates, Inc, La Jolla, CA 92037.
Davis
G R
GR
Stillman
C
C
Barringer
K
K
Cregg
J
J
Thill
G
G
D000429:Alcohol Oxidoreductases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D004271:DNA, Fungal / Q000235:genetics; D005800:Genes, Fungal; D008830:Microbodies / Q000201:enzymology; D008969:Molecular Sequence Data; D010843:Pichia / Q000201:enzymology / Q000235:genetics*; D010957:Plasmids; D015183:Restriction Mapping; D004718:Saccharomycetales / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D004271:DNA, Fungal; D000429:Alcohol Oxidoreductases; C019643:alcohol oxidase