Bone marrow transplantation in Polish conditions. Method for removal of red cells from the transplant in the case of major blood group incompatibility.
A modified polysaccharide-ditrizoate gradient centrifugation method is described. One hundred ml aliquots of marrow mixed 1:1 with medium is layered on 70 ml of polysaccharide-ditrizoate solution of specific density 1.077 in 5 x 19 cm flasks and centrifuged at 400 x g for 20 minutes at 20 degrees C. Interphase cells are collected and used for the transplant. The procedure is closed which reduces the danger of contamination, simple and takes between 2 and 3 hours. Two allogeneic HLA-identical transplants were performed using this technique and both resulted in full hematopoietic reconstitution and long term survival.
Wiktor-Jedrzejczak
W
W
Department of Immunology, Central Clinical Hospital CSK WAM, Warsaw.
Ratajczak
M Z
MZ
Szczylik
C
C
D001787:Blood Group Incompatibility; D016026:Bone Marrow Transplantation / Q000295:instrumentation / Q000379:methods*; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D002498:Centrifugation; D002648:Child; D002675:Child, Preschool; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D007223:Infant; D008297:Male