title: IL-3-dependent growth of basophil-like cells and mastlike cells from human bone marrow.
abstract: Human bone marrow cultured in the presence of human rIL-3 has been reported to give rise to basophils. In contrast, mouse bone marrow, cultured in the presence of mouse IL-3, leads to the growth of mast cells. To determine if human rIL-3 might also stimulate the growth of human mast cells, we cultured human bone marrow in the presence of human rIL-3 in suspension cultures, methylcellulose, and in "interphase" cultures where cells are layered over agar. The presence of mast cells was determined using a variety of histochemical techniques. In agreement with previous reports, basophil-like cells were identified in all culture systems. Mastlike cells were identified only in interphase cultures. By 3 wk, such cultures consisted of basophil-like cells (20 to 50%) and mastlike cells (1 to 5%). Cultures supplemented with rIL-4 showed no additional increase in basophil-like and mastlike cells. Both basophil-like and mastlike cells fluoresced with o-phthaldialdehyde and exhibited IgE receptors. Unlike basophil-like cells, mastlike cells were chloroacetate esterase, amidase, and human mast cell tryptase positive. We conclude that human rIL-3 can support the growth of human mastlike cells under selected culture conditions.
authors.affiliation: Mast Cell Physiology Section, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
authors.forename: A S
authors.initials: AS
authors.lastname: Kirshenbaum
authors.forename: J P
authors.initials: JP
authors.lastname: Goff
authors.forename: S C
authors.initials: SC
authors.lastname: Dreskin
authors.forename: A M
authors.initials: AM
authors.lastname: Irani
authors.forename: L B
authors.initials: LB
authors.lastname: Schwartz
authors.forename: D D
authors.initials: DD
authors.lastname: Metcalfe
mesh_terms: D001491:Basophils / Q000502:physiology*; D001853:Bone Marrow / Q000502:physiology*; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000502:physiology; D006801:Humans; D007377:Interleukin-3 / Q000494:pharmacology*; D007399:Interphase; D008407:Mast Cells / Q000201:enzymology / Q000502:physiology*; D010447:Peptide Hydrolases / Q000032:analysis; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D007377:Interleukin-3; D011994:Recombinant Proteins; D010447:Peptide Hydrolases; C023073:tosylarginine methyl ester hydrolase