Expression of ricin B chain in Escherichia coli.
DNA encoding ricin B chain was fused to that encoding the E. coli OmpA signal peptide using the expression secretion vector pIN-111-ompA. When induced, E. coli cells transformed with the recombinant plasmid express ricin B chain. The recombinant product accumulates in the periplasmic space in a soluble, biologically active form.
Hussain
K
K
Department of Biological Sciences, University of Warwick, Coventry, England.
Bowler
C
C
Roberts
L M
LM
Lord
J M
JM
D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D037121:Plant Lectins; D010947:Plants, Toxic; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification; D015183:Restriction Mapping; D012276:Ricin / Q000235:genetics* / Q000302:isolation & purification; D012278:Ricinus / Q000235:genetics*
D037121:Plant Lectins; D011993:Recombinant Fusion Proteins; D012276:Ricin