Autoradiographic evidence of estrogen binding sites in nuclei of diethylstilbesterol induced hamster renal carcinomas.
Estrogen binding sites were demonstrated by autoradiography in one transplantable and five primary diethylstilbesterol induced renal carcinomas in three hamsters. Radiolabelling, following the in vivo injection of 3H-17 beta estradiol, was increased only over the nuclei of tumor cells; stereologic analysis revealed a 4.5- to 6.7-times higher concentration of reduced silver grains over nuclei than cytoplasm of these cells. Despite rapid tubular excretion of estradiol which peaked in less than 1 h, the normal cells did not appear to bind the ligand. This is the first published report documenting the preferential in vivo binding of estrogen to nuclei of cells in estrogen induced hamster renal carcinomas.
Tse
J
J
Department of Pathology, University of Wisconsin, Medical School, Madison 53706.
Goldfarb
S
S
Pugh
T D
TD
D000818:Animals; D001345:Autoradiography; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D006224:Cricetinae; D003593:Cytoplasm / Q000378:metabolism; D004054:Diethylstilbestrol / Q000633:toxicity*; D004958:Estradiol / Q000378:metabolism*; D007680:Kidney Neoplasms / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008647:Mesocricetus; D051381:Rats; D011960:Receptors, Estrogen / Q000032:analysis*; D014316:Tritium
D011960:Receptors, Estrogen; D014316:Tritium; D004958:Estradiol; D004054:Diethylstilbestrol