title: Leupeptin alters the proteolytic processing of P126, the major parasitophorous vacuole antigen of Plasmodium falciparum.
abstract: Among several protease inhibitors tested, only leupeptin was found to modify qualitatively the processing of P126, a major antigen of the parasitophorous vacuole of Plasmodium falciparum, and to inhibit the release of merozoites. Whereas P126 is normally processed upon merozoite release into 2 polypeptides of 50 and 73 kDa which are discharged in the culture medium, leupeptin treatment led to the recovery of a 56 kDa fragment which was recognized by a monoclonal antibody specific for the 50 kDa polypeptide and of a 73 kDa fragment comigrating with the one obtained in normal culture conditions. Mild trypsinization of the 56 kDa polypeptide gave rise to a 50 kDa product the tryptic fragments of which comigrated with those of the 50 kDa antigen obtained from untreated cultures.
authors.affiliation: U. 42 INSERM, Villeneuve d'Ascq, France.
authors.forename: A
authors.initials: A
authors.lastname: Debrabant
authors.forename: P
authors.initials: P
authors.lastname: Delplace
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000378:metabolism*; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006868:Hydrolysis; D007163:Immunosorbent Techniques; D007976:Leupeptins / Q000494:pharmacology*; D009842:Oligopeptides / Q000494:pharmacology*; D010446:Peptide Fragments / Q000378:metabolism; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000378:metabolism; D011480:Protease Inhibitors / Q000494:pharmacology*; D014357:Trypsin
chemical_list: D000953:Antigens, Protozoan; D007976:Leupeptins; D009842:Oligopeptides; D010446:Peptide Fragments; D011480:Protease Inhibitors; D014357:Trypsin; C032854:leupeptin