Transforming growth factor-beta stimulates the expression of endothelin mRNA by vascular endothelial cells.
Endothelin is a potent vasoconstrictor peptide produced by vascular endothelial cells. Incubation of the serum-deprived confluent porcine aortic endothelial cells with 10-300 pM TGF-beta 1, resulted in a several fold increase in endothelin mRNA levels with a peak time of 2 h. An enzyme-linked immunosorbent assay revealed that the levels of endothelin in endothelial cell conditioned media was also increased by TGF-beta 1. These results suggest that TGF-beta 1, secreted by activated platelets, is involved not only in wound healing, but in the regulation of local vascular tone by stimulating endothelin production in the endothelial cells.
Kurihara
H
H
Third Department of Internal Medicine, University of Tokyo, Japan.
Yoshizumi
M
M
Sugiyama
T
T
Takaku
F
F
Yanagisawa
M
M
Masaki
T
T
Hamaoki
M
M
Kato
H
H
Yazaki
Y
Y
D000818:Animals; D001013:Aorta, Thoracic; D002478:Cells, Cultured; D016232:Endothelins; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D007700:Kinetics; D010455:Peptides / Q000235:genetics*; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics*; D013552:Swine; D014158:Transcription, Genetic / Q000187:drug effects*; D015291:Transforming Growth Factors / Q000494:pharmacology*
D016232:Endothelins; D010455:Peptides; D012333:RNA, Messenger; D015291:Transforming Growth Factors