Location of phosphorylation site and DNA-binding site of a positive regulator, OmpR, involved in activation of the osmoregulatory genes of Escherichia coli.
The OmpR protein of Escherichia coli is a positive regulator involved in activation of the ompF and ompC genes which encode the major outer membrane proteins OmpF and OmpC, respectively. By employing recombinant DNA techniques, we isolated the N- and C-terminal halves of the OmpR molecule. From the results of biochemical analyses of these fragments, it was concluded that the N-terminal portion contains a site involved in phosphorylation by an OmpR-specific protein kinase EnvZ, whereas the C-terminal part possesses a DNA-binding site for the ompC and ompF promoters.
Kato
M
M
Laboratory of Microbiology, School of Agriculture, Nagoya University, Japan.
Aiba
H
H
Tate
S
S
Nishimura
Y
Y
Mizuno
T
T
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001665:Binding Sites; D004269:DNA, Bacterial / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D010446:Peptide Fragments / Q000302:isolation & purification; D010766:Phosphorylation
D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; D010446:Peptide Fragments