Centromere proteins. I. Mitosis specific centromere antigen recognized by anti-centromere autoantibodies.
Human anti-centromere sera from scleroderma patients were used to detect centromere antigens of mouse fibroblast cells. An Mr = 59,000 centromere protein was localized exclusively on mitotic chromosomes. The association of this protein with the mitotic chromosomes proved to be DNase I sensitive. In interphase nuclei, this centromere antigen was not detectable by immunoblot techniques. The results suggest that the Mr = 59,000 mitosis specific protein may be necessary for the structural stability of kinetochores during mitosis.
Hadlaczky
G
G
Institute of Genetics, Hungarian Academy of Sciences, Szeged.
Praznovszky
T
T
Rasko
I
I
Kereso
J
J
D000818:Animals; D001323:Autoantibodies / Q000276:immunology*; D002478:Cells, Cultured; D002503:Centromere / Q000276:immunology*; D002875:Chromosomes / Q000032:analysis / Q000276:immunology*; D003850:Deoxyribonuclease I / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts; D005455:Fluorescent Antibody Technique; D006801:Humans; D015151:Immunoblotting; D051379:Mice; D008938:Mitosis; D011233:Precipitin Tests; D012259:Ribonuclease, Pancreatic / Q000378:metabolism
D001323:Autoantibodies; D003850:Deoxyribonuclease I; D012259:Ribonuclease, Pancreatic