Properties and cellular localization of a luciferin binding protein in the bioluminescence reaction of Gonyaulax polyedra.
A luciferin binding protein LBP involved in the bioluminescence reaction of Gonyaulax polyedra was purified and used for antibody production. Luciferin bound to LBP is fluorescent and can be used as a marker in living cells, allowing the localization of LBP in cortical organelles to be visualized. In cell sections, the same peripheral localization was observed using anti-LBP and immunofluorescence microscopy. The amount of LBP is ten-fold greater from cells from in night phase compared to those from in day phase, as determined both by immunoblots of cell extracts, and in vivo fluorescence. These changes correlate with the circadian changes in bioluminescence of living cells.
Morse
D
D
Harvard University, Department of Cellular and Developmental Biology, Cambridge, MA 02138.
Fritz
L
L
Pappenheimer
A M
AM
Hastings
J W
JW
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002478:Cells, Cultured; D004141:Dinoflagellida; D049454:Firefly Luciferin / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006863:Hydrogen-Ion Concentration; D008163:Luminescent Measurements
D002352:Carrier Proteins; D049454:Firefly Luciferin