Selected phenotypic and cloning properties of a bovine lymphoblastoid cell line, BL20.
A bovine lymphoblastoid cell line, BL20, was shown to express a BoLA antigen and surface IgM, implying that it was probably of B-cell origin. At low density (less than 10(5)/ml), the cells failed to grow. Inclusion of growth factor-containing supernatants from concanavalin A or pokeweed mitogen (PWM)-activated bovine lymphocytes or from thymus fibroblast-like cells did not improve cloning of the cells. Feeder cells also did not enhance cloning of the cells except for bovine thymus fibroblast-like cell monolayer.
Olobo
J O
JO
International Laboratory for Research on Animal Disease, Nairobi, Kenya.
Black
S J
SJ
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology; D002417:Cattle; D002455:Cell Division; D002460:Cell Line; D002470:Cell Survival; D002999:Clone Cells; D005347:Fibroblasts; D005455:Fluorescent Antibody Technique; D007136:Immunoglobulins / Q000032:analysis; D010641:Phenotype; D013950:Thymus Gland / Q000166:cytology
D000954:Antigens, Surface; D007136:Immunoglobulins