Quantity of DNA in the organs of adult Swiss male mice after AET and MEA treatment.
Adult Swiss male mice were injected intraperitoneally with 2-aminoethylesothiouronium bromide hydrobromide (AET) or cysteamine hydrochloride (MEA) in a dose of 400 mg/kg body weight. In the thirtieth, sixtieth or ninetieth minute after the injection, the animals were killed and the deoxyribonuclein acid content in 100 mg of fresh tissue of testes, spleen and liver, was measured. DNA was extracted from the organs by means of Burton's method, which is based on the estimation of deoxiribose content in the colour reaction with diphenylamine. The injection of AET and MEA did not distinctly influence the DNA content in the organs of mice. Statistically significant differences among the groups of mice were not observed compared to the controls, in mice treated with the compound, a decreasing tendency in the quantity of the DNA in the organs was found only.
Manowska
J
J
Department of Animal Physiology, Jagiellonian University, Cracow, Poland.
Mazur
L
L
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D003124:Colorimetry; D003543:Cysteamine / Q000494:pharmacology*; D004247:DNA / Q000187:drug effects*; D008099:Liver / Q000187:drug effects; D008297:Male; D051379:Mice; D009929:Organ Size / Q000187:drug effects; D013154:Spleen / Q000187:drug effects; D013737:Testis / Q000187:drug effects; D000338:beta-Aminoethyl Isothiourea / Q000494:pharmacology*
D000338:beta-Aminoethyl Isothiourea; D003543:Cysteamine; D004247:DNA