Insulin autoantibodies in non-obese diabetic (NOD) mice.
Anti-insulin autoantibodies were detected in NOD mice using ELISA. The antibodies were detected as early as at 5 weeks of age, long before onset of clinically overt diabetes, especially in diabetes-prone female mice. The anti-insulin specificity was verified by passage on affinity chromatography insulin columns and demonstration that the anti-insulin activity was located on the F(ab')2 region of the immunoglobulins. The presence of anti-insulin antibodies in prediabetic NOD mice provides a unique possibility for studying their significance and their eventual pathogenic role in the development of insulin-dependent diabetes.
Michel
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C
Service d'Immunologie Clinique, INSERM U 25, CNRS UA 122 Hôpital Necker, Paris, France.
Boitard
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C
Bach
J F
JF
D000818:Animals; D000918:Antibody Specificity; D001323:Autoantibodies / Q000032:analysis*; D003921:Diabetes Mellitus, Experimental / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007328:Insulin / Q000276:immunology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011236:Prediabetic State / Q000276:immunology
D001323:Autoantibodies; D007140:Immunoglobulin Fab Fragments; D007328:Insulin