Leukocyte types in cerebrospinal fluid and peripheral blood enumerated immunoenzymatically in aseptic meningitis and the Guillain-Barré syndrome.
To study celltype distribution simultaneously in peripheral blood (PB) and cerebrospinal fluid (CSF) from patients with aseptic meningitis (AM) (n = 14) and Guillain-Barré syndrome (GBS) (n = 9) we used an immunoenzymatic method that enabled the use of several monoclonal antibodies, also in CSF samples with normal cellcounts. In both patient groups a different cell-distribution in CSF compared to PB was found with regard to pan T cells (CD5+/anti-Leu1+), T cell subpopulations (CD4+/anti-Leu3+, CD8+/anti-Leu2+), B cells (OKB2+, OKB7+), monocytes/macrophages (CD11+/OKM1+) and HLA/DR expressing cells, whereas the distribution of HLA/DC+ cells was similar in CSF and PB. Thus, the CSF cell distribution does not reflect the distribution in PB. The proportion of T cells was higher and the proportion of B-cells was lower in CSF than in PB in both patient groups, which is a finding similar to that in patients with multiple sclerosis. The OKT9 marker, labelling proliferating cells expressing the transferrin receptor, was not useful as marker of local proliferation.
Ernerudh
J
J
Department of Neurology, University Hospital, Linköping, Sweden.
Fredriksson
S
S
Olsson
T
T
Forsberg
P
P
D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D007958:Leukocyte Count / Q000379:methods; D007962:Leukocytes / Q000145:classification* / Q000276:immunology; D008581:Meningitis / Q000473:pathology*; D008582:Meningitis, Aseptic / Q000097:blood / Q000134:cerebrospinal fluid / Q000473:pathology*; D008875:Middle Aged; D011129:Polyradiculoneuropathy / Q000097:blood / Q000134:cerebrospinal fluid / Q000473:pathology*
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation