Echinococcus multilocularis: in vitro secretion of antigen by hybridomas from metacestode germinal cells and murine tumor cells.
Hybrid cells were produced from Echinococcus multilocularis metacestode germinal cells and murine tumor cells. Small colonies were formed which, while ceasing to grow after a few generations, remained viable for at least 10 weeks. These hybridoma cells secrete antigen(s) reacting in indirect immunofluorescence and ELISA specifically with sera from patients suffering from an E. multilocularis infection. The antigen(s) appear suitable for the differential diagnosis of E. multilocularis and E. granulosus. Thus, hybridoma cells may produce helminth antigens.
Dieckmann-Schuppert
A
A
Department of Parasitology, University of Hohenheim, Stuttgart, Federal Republic of Germany.
Ruppel
A
A
Burger
R
R
Frank
W
W
D000818:Animals; D000947:Antigens, Helminth / Q000096:biosynthesis*; D015153:Blotting, Western; D004443:Echinococcosis / Q000276:immunology; D004446:Echinococcus / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007106:Immune Sera / Q000276:immunology; D051379:Mice; D009101:Multiple Myeloma; D014407:Tumor Cells, Cultured
D000947:Antigens, Helminth; D007106:Immune Sera