title: Identification and sequence analysis of a gene encoding gp67, an abundant envelope glycoprotein of the baculovirus Autographa californica nuclear polyhedrosis virus.
abstract: Monoclonal antibodies with specificity for the abundant envelope surface glycoprotein (gp67) of Autographa californica nuclear polyhedrosis virus (AcMNPV) were used to screen a lambda gt11 expression library of AcMNPV DNA fragments. The gp67 gene was mapped to the left end of the EcoRI H fragment in a right-to-left orientation on the consensus map of AcMNPV. A 2.1-kilobase transcript which hybridized to the region was first detected in cell extracts at 2 h postinfection; it peaked in abundance at 18 h postinfection and thereafter was present at lower levels. The nucleotide sequence of the region was determined, and a 1,590-nucleotide open reading frame flanked by an AT-rich sequence was identified that could encode a polypeptide with 529 amino acid residues (molecular mass of 60,167 daltons). Computer analysis indicated that the peptide possesses two hydrophobic regions near the N and C termini as well as six potential N-linked glycosylation sites. We suggest that following cleavage of a signal peptide, the polypeptide undergoes further processing and becomes anchored at its C terminus in the virus envelope. The final seven amino acid residues at the C terminus contain basic amino acids and may have a role in virion assembly.
authors.affiliation: Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Whitford
authors.forename: S
authors.initials: S
authors.lastname: Stewart
authors.forename: J
authors.initials: J
authors.lastname: Kuzio
authors.forename: P
authors.initials: P
authors.lastname: Faulkner
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001483:Base Sequence; D003001:Cloning, Molecular; D005814:Genes, Viral; D007304:Insect Viruses / Q000235:genetics*; D007313:Insecta / Q000382:microbiology; D008562:Membrane Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D012367:RNA, Viral / Q000235:genetics; D015183:Restriction Mapping; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D000911:Antibodies, Monoclonal; D008562:Membrane Glycoproteins; D012367:RNA, Viral; D014759:Viral Envelope Proteins