Regional distribution of immunoreactive endothelin in porcine tissue: abundance in inner medulla of kidney.
A specific and sensitive radioimmunoassay for endothelin has been developed. Half maximal inhibition of binding of radioiodinated endothelin was observed at 37 pg/tube and endothelin was detectable as low as 1 pg/tube. With this assay, the regional distribution of endothelin was determined in porcine tissue. The highest concentration of immunoreactive endothelin was observed in inner medulla of kidney (6.2 +/- 1.1 pg/mg wet weight), while the concentration in kidney cortex was very low. Immunoreactive endothelin was also found in lung in relatively high concentration. The immunoreactive endothelin in porcine lung and inner medulla of kidney was further characterized by reverse phase high performance liquid chromatography combined with radioimmunoassay.
Kitamura
K
K
First Department of Internal Medicine, Miyazaki Medical College, Japan.
Tanaka
T
T
Kato
J
J
Eto
T
T
Tanaka
K
K
D000818:Animals; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D016232:Endothelins; D004730:Endothelium, Vascular / Q000032:analysis*; D007679:Kidney Medulla / Q000032:analysis*; D008168:Lung / Q000032:analysis; D010455:Peptides / Q000276:immunology / Q000302:isolation & purification*; D011863:Radioimmunoassay / Q000379:methods; D013552:Swine; D014018:Tissue Distribution
D016232:Endothelins; D010455:Peptides