The neutrophil-activating protein (NAP-1) is also chemotactic for T lymphocytes.
T lymphocyte chemotactic factor (TCF) was purified to homogeneity from the conditioned media of phytohemagglutinin-stimulated human blood mononuclear leukocytes by a sequence of chromatography procedures. The amino-terminal amino acid sequence of the purified TCF showed identity with neutrophil-activating protein (NAP-1). Both TCF and recombinant NAP-1 (rNAP-1) were chemotactic for neutrophils and T lymphocytes in vitro supporting the identity of TCF with NAP-1. Injection of rNAP-1 into lymphatic drainage areas of lymph nodes in Fisher rats caused accelerated emigration of only lymphocytes in high endothelial venules. Intradermal injection of rNAP-1 caused dose-dependent accumulation of neutrophils and lymphocytes.
Larsen
C G
CG
Laboratory of Molecular Immunoregulation, National Cancer Institute, Frederick, MD 21701.
Anderson
A O
AO
Appella
E
E
Oppenheim
J J
JJ
Matsushima
K
K
D000818:Animals; D002630:Chemotactic Factors / Q000302:isolation & purification*; D002634:Chemotaxis, Leukocyte; D016209:Interleukin-8; D010455:Peptides / Q000302:isolation & purification*; D051381:Rats; D013601:T-Lymphocytes / Q000276:immunology*
D002630:Chemotactic Factors; D016209:Interleukin-8; D010455:Peptides