title: Characteristics of flow-mediated brachial artery vasodilation in human subjects.
abstract: In an effort to determine whether arterial conductance vessels dilate in response to increased blood flow stimuli, brachial artery area (cm2) and diameter (cm) were derived by simultaneous measurement of forearm blood flow (ml/min.100 ml) and brachial artery blood flow velocity (cm/sec) following the release of arterial occlusion. Measurements were made at rest and at the time of maximal flow after the release of graded periods of forearm arterial occlusion (20 seconds to 10 minutes). These studies showed a graded large vessel dilation following occlusions of up to 1 minute (baseline diameter, 0.33 +/- 0.01; after 1 minute occlusion, 0.45 +/- 0.02 cm; p less than 0.05) after which time diameter plateaued (after 10 minutes of occlusion, 0.48 +/- 0.02 cm). In addition, the time course of diameter and flow changes after 3 minutes of arterial occlusion were examined. Flow was maximal at 5 seconds but diameter was maximal at 15-30 seconds after release. Furthermore, the half time for the return of diameter to baseline was longer than that for blood flow. We also measured the diameter after forearm heating (42 degrees C) and noted a substantial increase in diameter (before heating, 0.32 +/- 0.01; after heating, 0.39 +/- 0.02 cm; p less than 0.05). Finally, we applied pressure to the venous side of arteriovenous fistulae in five hemodialysis patients. This maneuver was associated with large reductions in forearm blood flow (baseline flow, 63.3 +/- 10.6; venous compression flow, 36.0 +/- 4.4 ml/min.100 ml; p less than 0.05) and a decrease in brachial artery size (baseline diameter, 0.63 +/- 0.07; venous compression diameter, 0.58 +/- 0.06 cm; p less than 0.05). We conclude that 1) the human brachial artery size changes in response to changes in blood flow, and 2) the maximal dilation occurs after maximal flow is noted. Although alternate explanations are possible for each of our observations, our results are most consistent with a flow-mediated, localized vasodilating process.
authors.affiliation: Division of Cardiology, Milton S. Hershey Medical Center, Pennsylvania State University, Hershey 17033.
authors.forename: L I
authors.initials: LI
authors.lastname: Sinoway
authors.forename: C
authors.initials: C
authors.lastname: Hendrickson
authors.forename: W R
authors.initials: WR
authors.lastname: Davidson
authors.forename: S
authors.initials: S
authors.lastname: Prophet
authors.forename: R
authors.initials: R
authors.lastname: Zelis
mesh_terms: D000328:Adult; D001783:Blood Flow Velocity; D001916:Brachial Artery / Q000502:physiology*; D003250:Constriction; D005542:Forearm / Q000098:blood supply; D006801:Humans; D007511:Ischemia / Q000503:physiopathology; D012039:Regional Blood Flow; D012146:Rest; D014463:Ultrasonography; D014664:Vasodilation