Cellular infiltration in induced rat thyroiditis: phenotypic analysis and relationship to genetic restriction.
We have investigated the responsiveness to thyroglobulin (Tg) plus complete Freund's adjuvant (CFA) and B. pertussis in a variety of inbred and MHC congenic strains of rats in terms of both Tg-autoantibody titres and histological thyroiditis index. Severity of thyroiditis was strongly Tg-dependent and closely related to the RT.1-MHC haplotype. Phenotypic examination of the inflammatory thyroid infiltrate using single and double indirect immunofluorescence techniques revealed a high proportion of macrophages and T lymphocytes, mainly of the cytotoxic/suppressor subset, in the high responder strains. Thyroid epithelial class II MHC expression although not prominent was strain-restricted and related to the amount of Ia+ leukocyte infiltrate.
De Assis-Paiva
H J
HJ
Department of Immunology, University College and Middlesex School of Medicine, London, UK.
Rayner
D C
DC
Roitt
I M
IM
Cooke
A
A
D000818:Animals; D001323:Autoantibodies / Q000096:biosynthesis; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D005455:Fluorescent Antibody Technique; D005620:Freund's Adjuvant; D006239:Haplotypes; D000949:Histocompatibility Antigens Class II / Q000235:genetics; D007958:Leukocyte Count; D008264:Macrophages / Q000276:immunology; D010641:Phenotype; D051381:Rats; D011919:Rats, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology; D013954:Thyroglobulin / Q000276:immunology; D013961:Thyroid Gland / Q000473:pathology; D013967:Thyroiditis, Autoimmune / Q000235:genetics / Q000276:immunology* / Q000473:pathology
D001323:Autoantibodies; D000949:Histocompatibility Antigens Class II; D005620:Freund's Adjuvant; D013954:Thyroglobulin