[The fluorescence of mouse crystalline lenses at different stages of radiation cataract studied by synchronous scanning].
Fluorescence of intact lenses of F1 (CBA x C57BL6) mice at different stages of X-ray cataract induced by gamma irradiation (5.00 Gr) has been studied by synchronous scanning of fluorescence, the shift between emission and excitation wave lengths being 20 nm. The ratio between peek intensities of the nontryptophan and tryptophan fluorescence within the synchronous scanning spectra (K) has increased 3.5 times as much at the stage of singular dot-like opacities. K-parameter correlated with GSH level in the lenses (r = -0.9). According to the results achieved, K could be regarded as an informative indicator of the development of X-ray cataract at the stage previous to turbidity.
Aĭtmagambetova
M T
MT
Deev
A I
AI
Kabachenko
A N
AN
Vladimirov
Iu A
IuA
D000818:Animals; D002386:Cataract / Q000209:etiology / Q000378:metabolism*; D005978:Glutathione / Q000032:analysis / Q000378:metabolism / Q000528:radiation effects; D007908:Lens, Crystalline / Q000032:analysis / Q000528:radiation effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011833:Radiation Injuries, Experimental / Q000150:complications / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D014916:Whole-Body Irradiation
D005978:Glutathione
Izuchenie fliuorestsentsii khrustalikov mysheĭ na razlichnykh stadiiakh radiatsionnoĭ katarakty metodom sinkhronnogo skanirovaniia.