Hypothermia or continuous ventilation decreases ischemia-reperfusion injury in an ex vivo rat lung model.
The susceptibility of lung tissue to ischemia-reperfusion injury has made distant procurement of heart-lung allografts difficult. The effects of hypothermia, ventilation without perfusion, and various reperfusion solutions (PSS/Ficoll or whole blood) on the development of ischemia-reperfusion lung injury were investigated. Use of an ex vivo rat lung model in which the above variables were individually varied permitted a direct approach for these studies. Normothermic ischemia for 1 hour caused significant damage, documented by increased iodine 125 bovine serum albumin (125I-BSA) in alveolar lavage fluid and lung parenchyma compared with nonischemic controls. Hypothermic (4 degrees C) ischemia for 4 hours in lungs reperfused with salt solution and for as many as 12 hours in lungs reperfused with whole blood caused no significant increase in 125I-BSA in alveolar lavage fluid and lung parenchyma compared with nonischemic controls. Lungs ventilated without perfusion showed no increase in 125I-BSA leakage compared with controls. The ex vivo rat lung model is excellent for studying ischemia-reperfusion injury. It is reproducible, allows for variance of reperfusion solutions, and permits change in temperature and ventilation easily.
Badellino
M M
MM
Department of Internal Medicine, University of Michigan, Ann Arbor 48109.
Morganroth
M L
ML
Grum
C M
CM
Lynch
M J
MJ
Bolling
S F
SF
Deeb
G M
GM
D000818:Animals; D004195:Disease Models, Animal; D005069:Evaluation Studies as Topic; D007036:Hypothermia, Induced; D007511:Ischemia / Q000150:complications / Q000517:prevention & control*; D008168:Lung / Q000098:blood supply*; D008297:Male; D011175:Positive-Pressure Respiration; D011650:Pulmonary Alveoli / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D015427:Reperfusion Injury / Q000209:etiology / Q000517:prevention & control*; D012710:Serum Albumin, Bovine / Q000032:analysis; D012965:Sodium Chloride / Q000008:administration & dosage
D012710:Serum Albumin, Bovine; D012965:Sodium Chloride