title: [Antigen-antibody complex dissociation in affinity chromatography].
abstract: Different methods of the elution of antibodies from an immunosorbent column have been studied, the serological activity and affinity of eluted antibodies have been evaluated in the enzyme immunoassay. The activity of conjugates (peroxidase-labeled antibodies) has been shown to depend not only on the serological activity and affinity of antibodies, but also on the method of binding antibodies with the enzyme.
authors.forename: E A
authors.initials: EA
authors.lastname: Shkurina
authors.forename: L I
authors.initials: LI
authors.lastname: Vaneeva
mesh_terms: D000327:Adsorption; D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification; D000915:Antibody Affinity; D000936:Antigen-Antibody Complex / Q000032:analysis*; D002846:Chromatography, Affinity / Q000379:methods*; D002852:Chromatography, Ion Exchange; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000302:isolation & purification; D011817:Rabbits
chemical_list: D000906:Antibodies; D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G
vernacular_title: Izuchenie dissotsiatsii kompleksa antigen-antitelo v affinnoĭ khromatografii.