title: Conditions affecting the mutagenicity of trichloroethylene in Salmonella.
abstract: Trichloroethylene (TCE) is a high production volume chemical frequently stabilized with oxiranes. These oxiranes may be responsible for the mutagenic activity of TCE in Salmonella, which has been occasionally, but not consistently, reported. High purity and oxirane-stabilized TCE samples were tested for their mutagenic potential in Salmonella typhimurium strains TA 1535, TA 98, and TA 100. Stabilized TCE was tested using a preincubation protocol up to a dose level of 10,000 micrograms per plate, but no mutagenic response was observed in either the presence or absence of a supplementary metabolic activation system (S9 mix) derived from Aroclor 1254-induced male rat liver. TCE without oxirane stabilizers also was nonmutagenic when tested in a vapor delivery system at nominal concentrations of up to 20% and using S9 mix derived from either rat or hamster. TCE containing 0.5-0.6% 1,2-epoxybutane did induce mutagenic responses from strains TA 1535 and TA 100 in the presence and absence of S9 mix. The lowest effective dose was about 0.63% in TA 1535 in the absence of S9 mix. Vapor-phase tests with 1,2-epoxybutane showed that an atmospheric concentration of 0.009% could induce 12-fold and 3-fold increases, respectively, in strains TA 1535 and TA 100. These increases would account for the mutagenic activity of the stabilized TCE sample. Epichlorohydrin (another commonly used stabilizer) induced similar increases in mutant numbers at an atmospheric concentration of 0.0009%. The absence of a significant response caused by unstabilized TCE in the presence of S9 mix is probably due to a lack of assay sensitivity, since chloral, a metabolite of TCE, is a mutagen in TA 100 [Haworth et al.: Environ Mutagen [Supplement 1] 5:3-142, 1983].
authors.affiliation: Department of Mutagenesis and Cellular Toxicology, Inveresk Research International, Musselburgh, Scotland.
authors.forename: D B
authors.initials: DB
authors.lastname: McGregor
authors.forename: D M
authors.initials: DM
authors.lastname: Reynolds
authors.forename: E
authors.initials: E
authors.lastname: Zeiger
mesh_terms: D000818:Animals; D001681:Biological Assay; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D004811:Epichlorohydrin / Q000633:toxicity; D004852:Epoxy Compounds / Q000633:toxicity; D008099:Liver; D008647:Mesocricetus; D009152:Mutagenicity Tests; D009154:Mutation; D051381:Rats; D011916:Rats, Inbred F344; D012486:Salmonella typhimurium; D014241:Trichloroethylene / Q000633:toxicity*
chemical_list: D004852:Epoxy Compounds; D004811:Epichlorohydrin; C027488:1,2-epoxybutane; D014241:Trichloroethylene