Induction of glutathione S-transferase by phenobarbital in rat hepatocyte culture.
The in vitro effect of phenobarbital on glutathione S-transferase (GST) activity toward 1-chloro-2,4-dinitrobenzene (CDNB) in rat hepatocyte culture was investigated. Treatment of hepatocytes with a medium containing 6 or 8 mM phenobarbital for 20 h increased the specific activity of GST approx. 140% and 160% of control, respectively. When induction of GST by phenobarbital treatment was observed, the cellular level of phenobarbital determined by HPLC was approx. 10 - 15 micrograms/mg of 20000 x g supernatant protein of hepatocytes. Thus, an inductive effect of phenobarbital on GST was observed in vitro as well as in rat liver. It was suggested that a certain level of cellular phenobarbital was necessary for the induction.
Sasaki
H
H
Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
Miyaura
S
S
Horie
K
K
Isono
H
H
D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction; D005982:Glutathione Transferase / Q000096:biosynthesis* / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D008297:Male; D010634:Phenobarbital / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D005982:Glutathione Transferase; D010634:Phenobarbital