title: A human airway smooth muscle cell line that retains physiological responsiveness.
abstract: We report the development of a nontransformed line of human airway smooth muscle cells retaining smooth muscle-specific contractile protein expression and physiological responsiveness to agonists implicated in inflammatory airway diseases. Specific responses to histamine, leukotrienes, bradykinin, platelet-activating factor, substance P, and thromboxane analogues are demonstrated as well as functional coupling to beta-adrenergic receptors. The cell line was characterized using indirect immunofluorescence, as well as electrophoretic separation and immunoblot analysis of smooth muscle-specific actin. Functional responses were assessed by measurements of cytosolic calcium and stimulation of adenosine 3',5'-cyclic monophosphate production. The cells retain their responsiveness over many population doublings and should be a useful model to examine specific receptor-effector mechanisms, as well as the effects of neurohumoral agents on the regulation of airway smooth muscle growth and differentiation.
authors.affiliation: Department of Medicine, University of Pennsylvania, Philadelphia 19104-6046.
authors.forename: R A
authors.initials: RA
authors.lastname: Panettieri
authors.forename: R K
authors.initials: RK
authors.lastname: Murray
authors.forename: L R
authors.initials: LR
authors.lastname: DePalo
authors.forename: P A
authors.initials: PA
authors.lastname: Yadvish
authors.forename: M I
authors.initials: MI
authors.lastname: Kotlikoff
mesh_terms: D000199:Actins / Q000032:analysis; D000205:Actomyosin / Q000032:analysis; D002118:Calcium / Q000378:metabolism; D002455:Cell Division; D002460:Cell Line; D046508:Culture Techniques / Q000379:methods; D003600:Cytosol / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D007700:Kinetics; D008970:Molecular Weight; D009130:Muscle, Smooth / Q000502:physiology*; D014132:Trachea / Q000502:physiology
chemical_list: D000199:Actins; D000205:Actomyosin; D002118:Calcium