Phenytoin and phenobarbital concentrations in serum: a comparison of Ames Seralyzer with GLC, TDX, and EMIT.
A recently developed system for measuring antiepileptic drug concentrations was evaluated for phenytoin and phenobarbital. The apoenzyme reactive immunoassay system was compared with gas-liquid chromatography, EMIT, and TDX systems. Comparisons were performed with control specimens and with sera obtained from patients at three facilities. Precision for all methods was similar, with within-run and between-run coefficients of variation generally 5%. The accuracy of all methods was acceptable, but bias was present in some. However, measurements obtained by a nontechnical person (physician) in a clinical setting were sometimes inaccurate.
Leppik
I E
IE
Ramsey Clinic, St. Paul, Minnesota.
Oles
K S
KS
Sheehan
M L
ML
Penry
J K
JK
Parker
D R
DR
Gallagher
T K
TK
Caron
G P
GP
Rosenfeld
W
W
Salmon
J J
JJ
D002849:Chromatography, Gas; D006801:Humans; D007124:Immunoenzyme Techniques; D015337:Multicenter Studies as Topic; D010634:Phenobarbital / Q000097:blood*; D010672:Phenytoin / Q000097:blood*
D010672:Phenytoin; D010634:Phenobarbital