title: Preparation of Escherichia coli elongation factor Tu-guanosine 5'-triphosphate analogs.
abstract: A simple procedure for the bulk preparation of 20 mg of Escherichia coli elongation factor (EF)-Tu-GTP analogs is described. The protocol is based upon the preparation and stabilization of nucleotide-free EF-Tu using an EF-Ts affinity chromatographic resin. The procedure is a general one for the preparation of any GTP analog of EF-Tu.
authors.affiliation: Department of Biochemistry, University of California, Riverside 92521.
authors.forename: K
authors.initials: K
authors.lastname: Delaria
authors.forename: F
authors.initials: F
authors.lastname: Jurnak
mesh_terms: D001667:Binding, Competitive; D002846:Chromatography, Affinity; D004926:Escherichia coli; D006150:Guanine Nucleotides / Q000302:isolation & purification; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D010444:Peptide Elongation Factor Tu / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D010445:Peptide Elongation Factors; D012116:Resins, Plant; D012685:Sepharose
chemical_list: D006150:Guanine Nucleotides; D010445:Peptide Elongation Factors; D012116:Resins, Plant; C023512:elongation factor Ts; D006160:Guanosine Triphosphate; D012685:Sepharose; D010444:Peptide Elongation Factor Tu