Secretion of novel and homologous neutrophil-activating peptides by LPS-stimulated human endothelial cells.
Human umbilical vein endothelial cells in culture produce two chemotactic polypeptides when stimulated with LPS. The chemotactic factors could be purified to apparent homogeneity by HPLC techniques and were identified as 7.5-kDa and 15-kDa polypeptides by SDS-PAGE under nonreducing conditions. Both factors are potent chemotaxins for human neutrophils demonstrating half-maximal chemotaxis at 2 ng/ml and g ng/ml, respectively. In addition both peptides elicited release of azurophilic granule constituents when neutrophils were pretreated with cytochalasin B. Cross-desensitization experiments by using human neutrophils revealed cross-reactivities between both chemotaxins, not, however, with C5a or FMLP, indicating that both endothelial cell-derived neutrophil activating peptides (ENAP) are homologous. In addition, the 7.5-kDa factor (beta-ENAP) proved to be the quantitatively dominating and more potent chemotaxin as compared to the 15 kDa factor (alpha-ENAP). beta-ENAP shows biochemical and biologic similarities to monocyte- and lymphocyte-derived neutrophil-activating peptides MONAP and LYNAP, which recently were purified and sequenced.
Schröder
J M
JM
Department of Dermatology, University of Kiel, FRG.
Christophers
E
E
D002630:Chemotactic Factors / Q000302:isolation & purification / Q000378:metabolism* / Q000502:physiology; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D003470:Culture Media / Q000032:analysis; D004730:Endothelium, Vascular / Q000032:analysis / Q000502:physiology*; D006801:Humans; D016209:Interleukin-8; D007700:Kinetics; D008070:Lipopolysaccharides; D009504:Neutrophils / Q000502:physiology*; D010455:Peptides / Q000302:isolation & purification / Q000378:metabolism* / Q000502:physiology
D002630:Chemotactic Factors; D003470:Culture Media; D016209:Interleukin-8; D008070:Lipopolysaccharides; D010455:Peptides