title: A method for the efficient transfer of isolated mitochondria into yeast protoplasts.
abstract: When protoplasts isolated from non-respiring rho0 strains of S. cerevisiae are incubated with mitochondria from respiring rho+ strains in the presence of polyethylene glycol, a small fraction of the acceptor protoplasts acquires respiratory capacity. Pretreatment of the isolated mitochondria and protoplasts with Ca2+ ions enhanced the frequency of ensuing respiration-competent colonies by almost three orders of magnitude. When mitochondria were isolated from a strain exhibiting killer capacity as a cytoplasmic marker, in addition to the appropriate nuclear and mitochondrial markers, about one-third of the ensuing rho+ colonies were not killers. Possible mechanisms for the transfer of mitochondria into acceptor protoplasts were studied.
authors.affiliation: Institute of Animal Physiology, Slovak Academy of Sciences, Ivanka pri Dunaji, Czechoslovakia.
authors.forename: P
authors.initials: P
authors.lastname: Sulo
authors.forename: P
authors.initials: P
authors.lastname: Griac
authors.forename: V
authors.initials: V
authors.lastname: Klobucníková
authors.forename: L
authors.initials: L
authors.lastname: Kovác
mesh_terms: D005819:Genetic Markers; D008928:Mitochondria / Q000378:metabolism / Q000502:physiology*; D010101:Oxygen Consumption; D010641:Phenotype; D011523:Protoplasts / Q000378:metabolism / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000648:ultrastructure*
chemical_list: D005819:Genetic Markers