Desensitization and recovery of prostaglandin-stimulated adenylate cyclase activity in a murine virus-induced T lymphoma cell line BL/VL3.
Prolonged (16 h) preexposure to prostaglandin E1 (PGE1) of cells from a murine virus-induced T lymphoma cell line BL/VL3 provoked, in their membranes, a dose-dependent reduction of PGE1-mediated adenylate cyclase stimulation. Smaller (but significant) decreases of helodermin- and isoproterenol-mediated stimulations were also observed. After a 16 h incubation of these cells with 1 microM PGE1, that reduced by 85%, the PGE1-mediated adenylate cyclase stimulation in membranes, 50% of the PGE1 response recovered after 2 h of PGE1 withdrawal from the incubation medium. Over the following 2-24 h time interval, further recovery was limited. Protein synthesis was required for this resensitization mechanism of functional PGE1 receptors coupled to adenylate cyclase, as judged by the inhibitory effects of cycloheximide.
Abello
J
J
Department of Biochemistry and Nutrition, School of Medicine, Université Libre de Bruxelles, Belgium.
Robberecht
P
P
Damien
C
C
Christophe
J
J
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000527:Alprostadil / Q000502:physiology*; D000818:Animals; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D003513:Cycloheximide / Q000494:pharmacology; D008223:Lymphoma; D051379:Mice; D011982:Receptors, Prostaglandin / Q000187:drug effects / Q000502:physiology; D013601:T-Lymphocytes / Q000201:enzymology*
D011982:Receptors, Prostaglandin; D003513:Cycloheximide; D000262:Adenylyl Cyclases; D000527:Alprostadil