Modulatory effects of adrenergic agonists on testosterone secretion from rat dispersed testicular cells or Percoll-purified Leydig cells.
1. Freshly dispersed testicular interstitial cells as well as Percoll-purified Leydig cells were studied in vitro in order to evaluate the effect of adrenergic agonists on testosterone (T) secretion. 2. Epinephrine and phenylephrine did not change the rate of T release under basal conditions in freshly dispersed interstitial cells, but enhanced it during human chorionic gonadotropin (hCG) stimulation. Norepinephrine and clonidine had no effect on T secretion. 3. In contrast, in Percoll-purified Leydig cells epinephrine increased T release both under basal and hCG-stimulated conditions. 4. These data demonstrate that neurotransmitters may participate in T secretion from isolated Leydig cells.
Wanderley
M I
MI
Departamento di Fisiologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Brasil.
Favaretto
A L
AL
Valença
M M
MM
Hattori
M
M
Wakabayashi
K
K
Antunes-Rodrigues
J
J
D000818:Animals; D002478:Cells, Cultured; D003000:Clonidine / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology*; D066298:In Vitro Techniques; D007985:Leydig Cells / Q000378:metabolism*; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology; D010656:Phenylephrine / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013739:Testosterone / Q000378:metabolism*
D010656:Phenylephrine; D013739:Testosterone; D003000:Clonidine; D009638:Norepinephrine; D004837:Epinephrine