[Cyanocobalamin Reference Standard (Control 871) of National Institute of Hygienic Sciences].
Cyanocobalamin was tested for the preparation of "Cyanocobalamin Reference Standard (Control 871)". Analytical data obtained were as follows: loss on drying, 11.6%; infrared spectrum, same as that of Cyanocobalamin Reference Standard (Control 861); thin-layer chromatography, two contaminants were detected; high-performance liquid chromatography, seven contaminants were detected; assay, 99.9% by JP XI method and 100.2% by HPLC method in terms of J.P. Reference Standard (Control 861), respectively. On the basis of those results, this material was authorized as the Japanese Pharmacopoeia Standard (Control 871).
Tanaka
M
M
Hiroshige
R
R
Murai
M
M
Tokunaga
H
H
Okada
S
S
Kimura
T
T
D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004340:Drug Contamination; D007564:Japan; D010602:Pharmacopoeias as Topic / Q000592:standards*; D012015:Reference Standards; D013055:Spectrophotometry, Infrared; D014805:Vitamin B 12 / Q000032:analysis / Q000592:standards*
D014805:Vitamin B 12