title: Metabolic changes due to the in vitro addition of estradiol in rat hepatocytes.
abstract: Short-term effects of estradiol on gluconeogenesis, redox state and on the activities of the enzymes involved in NADPH production have been examined. Hepatocytes incubated with estradiol (10(-4)M) showed a decreased gluconeogenesis and an increased lactate/pyruvate ratio. The malic enzyme was found to be stimulated by 45%, whereas glucose-6-phosphate dehydrogenase and isocitrate dehydrogenase activities were not affected by the presence of the hormone. Estradiol produced selective inhibitions of glucose synthesis from various substrates and diminished malate dehydrogenase activity by 22%. The possibility that the estradiol-induced alterations here reported are related to the hormone catabolism itself in the liver is suggested. Other results in this work call attention to the importance of the vehicle used for the steroid dispersion. Propylene glycol markedly alters the metabolic state of liver cells and also antagonizes the modifications produced by estradiol.
authors.affiliation: Departamento de Fisiología, Facultad de Medicina y Odontología, Universidad del País Vasco, Bilbao, Spain.
authors.forename: A M
authors.initials: AM
authors.lastname: Astiazarán
authors.forename: Y
authors.initials: Y
authors.lastname: Chico
authors.forename: M B
authors.initials: MB
authors.lastname: Ruiz
authors.forename: M T
authors.initials: MT
authors.lastname: Alonso
authors.forename: B
authors.initials: B
authors.lastname: Ochoa
authors.forename: M
authors.initials: M
authors.lastname: Lacort
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005943:Gluconeogenesis / Q000187:drug effects*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D011409:Propylene Glycols / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D011409:Propylene Glycols; D004958:Estradiol; D008291:Malate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase