title: Technetium-99m DTPA does not break down during ultrasound nebulization.
abstract: For several years aerosolized 99Tcm-DTPA has been used for studies of pulmonary permeability. Comparable results have been obtained regardless of the 99Tcm-DTPA having been nebulized in an ultrasound nebulizer or a jet nebulizer. It was therefore surprising when in a recent study it was observed that more than 90% of a commercially available 99Tcm-DTPA (Squibb) was dissociated into 99TcmO4- and DTPA during ultrasound nebulization. To verify this observation we performed chromatography of seven commercially available DTPA-labelling kits (including that of Squibb). Four different test preparations of each of the kits were analysed: (1) stock solution of 99Tcm-DTPA; (2) heated (80 degrees C) 99Tcm-DTPA; (3) 99Tcm-DTPA nebulized by a DeVilbiss ultrasound nebulizer; (4) 99Tcm-DTPA nebulized by a Varic ultrasound nebulizer. In no case were we able to demonstrate more than 1.0% reduced pertechnetate (99TcmO2) or more than 0.9% free 99TcmO4- after either heating or ultrasound nebulization. A maximum of 5.6% 99TcmO2 could be demonstrated in the stock solution if chromatography was done immediately after the preparation, but only 15 min later no more than 0.9% 99TcmO2 was present. We conclude that there is no essential chemical breakdown of 99Tcm-DTPA during ultrasound nebulization. Caution should be taken not to use the 99Tcm-DTPA until 15 min after its preparation.
authors.affiliation: Department of Clinical Physiology and Nuclear Medicine, Rigshospitalet, Copenhagen, Denmark.
authors.forename: S
authors.initials: S
authors.lastname: Groth
authors.forename: H
authors.initials: H
authors.lastname: Funch-Rosenberg
authors.forename: J
authors.initials: J
authors.lastname: Mortensen
authors.forename: N
authors.initials: N
authors.lastname: Rossing
mesh_terms: D000336:Aerosols; D002199:Capillary Permeability; D004355:Drug Stability; D006801:Humans; D009330:Nebulizers and Vaporizers; D009942:Organometallic Compounds / Q000008:administration & dosage / Q000032:analysis*; D004369:Pentetic Acid / Q000008:administration & dosage / Q000032:analysis*; D013667:Technetium / Q000032:analysis*; D016284:Technetium Tc 99m Pentetate; D014465:Ultrasonics
chemical_list: D000336:Aerosols; D009942:Organometallic Compounds; D013667:Technetium; D004369:Pentetic Acid; D016284:Technetium Tc 99m Pentetate