Comparative field evaluation of the fluorescent-antibody test, virus isolation from tissue culture, and enzyme immunodiagnosis for rapid laboratory diagnosis of rabies.
The rabies tissue culture infection test (RTCIT) and rapid rabies enzyme immunodiagnosis (RREID) were compared to the fluorescent-antibody test (FAT) with field specimens. At the French National Reference Center for Rabies, 15,248 specimens were analyzed by FAT and RTCIT, and 2,290 of those specimens were also tested by RREID; 818 other specimens were tested by FAT and RREID in 12 laboratories located in Africa, Asia, and Latin America. The sensitivities and specificities of RREID and RTCIT were comparable. This study showed that both tests can be used as backup procedures to confirm FAT. RREID is also strongly recommended for epidemiological studies and for laboratories which are not equipped for performing FAT.
Bourhy
H
H
National Reference Center for Rabies, World Health Organization Collaborating Centre for Reference and Research on Rabies, Paris, France.
Rollin
P E
PE
Vincent
J
J
Sureau
P
P
D000349:Africa; D000818:Animals; D001208:Asia; D001933:Brain Stem / Q000382:microbiology; D002540:Cerebral Cortex / Q000382:microbiology; D046508:Culture Techniques; D005455:Fluorescent Antibody Technique; D006624:Hippocampus / Q000382:microbiology; D007124:Immunoenzyme Techniques; D007843:Latin America; D011237:Predictive Value of Tests; D011818:Rabies / Q000175:diagnosis / Q000662:veterinary*; D011820:Rabies virus / Q000276:immunology / Q000302:isolation & purification*; D013053:Spectrophotometry