A yeast protein possesses the DNA-binding properties of the adenovirus major late transcription factor.
The adenovirus major late transcription factor (MLTF), or upstream stimulatory factor, is a human promoter-specific transcription factor which recognizes the near-palindromic sequence GGCCACGTGACC (R. W. Carthew, L. A. Chodosh, and P. A. Sharp, Cell 43:439-448, 1985; L. A. Chodosh, R. W. Carthew, and P. A. Sharp, Mol. Cell. Biol. 6:4723-4733, 1986; M. Sawadogo and R. G. Roeder, Cell 43:165-175, 1985). We describe here a protein found in the yeast Saccharomyces cerevisiae which possesses DNA-binding properties that are virtually identical to those of human MLTF. These two proteins recognize the same DNA-binding site, make the same purine nucleotide contacts, and are affected in the same manner by mutations in the MLTF-binding site.
Chodosh
L A
LA
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139.
Buratowski
S
S
Sharp
P A
PA
D000256:Adenoviridae / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D005075:Biological Evolution; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D006801:Humans; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013045:Species Specificity; D014157:Transcription Factors / Q000378:metabolism*; D014764:Viral Proteins
D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C051096:MLTF protein, adenovirus; D014157:Transcription Factors; D014764:Viral Proteins; D004247:DNA