title: A cDNA clone encoding part of the major 25,000-dalton surface membrane antigen of adult Schistosoma mansoni.
abstract: Immunoscreening of an adult Schistosoma mansoni cDNA expression library, using antibodies raised against purified adult worm tegumental surface membranes, identified a recombinant clone containing a 141-bp insert. Antibodies raised against the recombinant antigen bound specifically to the tegument of adult worms and immunoprecipitated the major 25,000-dalton surface membrane antigen as well as a 22,000-dalton nascent polypeptide generated by cell-free translation of adult S. mansoni mRNA. The mature 25,000-dalton antigen was found to be precipitated by antibodies from infected mice, rats and humans.
authors.affiliation: Division of Parasitology, National Institute for Medical Research, Mill Hill, London, UK.
authors.forename: M
authors.initials: M
authors.lastname: Knight
authors.forename: C
authors.initials: C
authors.lastname: Kelly
authors.forename: V
authors.initials: V
authors.lastname: Rodrigues
authors.forename: X
authors.initials: X
authors.lastname: Yi
authors.forename: A
authors.initials: A
authors.lastname: Wamachi
authors.forename: S R
authors.initials: SR
authors.lastname: Smithers
authors.forename: A J
authors.initials: AJ
authors.lastname: Simpson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D000947:Antigens, Helminth / Q000235:genetics* / Q000276:immunology; D000954:Antigens, Surface / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D012550:Schistosoma mansoni / Q000235:genetics* / Q000276:immunology
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D000954:Antigens, Surface; D004247:DNA