Testis-specific histone H1t is truly a testis-specific variant and not a meiotic-specific variant.
Immunoblotting studies using highly specific polyclonal anti-histone H1t-IgG, which was extensively characterized by us previously, did not produce a signal with any of the histone H1 subtypes of either 1-day-old or adult rat ovarian nuclei. The absence of histone H1t in ovarian nuclei was also confirmed by indirect immunofluorescence studies. It is concluded, therefore, that histone H1t is truly a testis-specific histone variant and not a meiotic-specific variant.
Markose
E R
ER
Department of Biochemistry, Indian Institute of Science, Bangalore, India.
Rao
M R
MR
D000818:Animals; D015153:Blotting, Western; D005260:Female; D005455:Fluorescent Antibody Technique; D006657:Histones / Q000276:immunology / Q000502:physiology*; D007158:Immunologic Techniques; D008297:Male; D008540:Meiosis; D010053:Ovary / Q000502:physiology*; D051381:Rats; D013737:Testis / Q000502:physiology*
D006657:Histones