The LFA-1 antigen in human B lymphocyte activation.
Human tonsil B cells include a subpopulation (30 per cent) of cells which lack LFA-1 antigen. Activation of tonsil B cells by culture with anti-IgM and interleukin-4 led to an increase in staining intensities and in the proportion of cells staining, until by 48 h the majority of B cells were positive. Culture of activated cells with low-molecular weight B cell growth factor, which induces a proportion of cells to proliferate, led to a minor further increase in expression of the LFA-1 antigen. Inclusion of a monoclonal antibody against the LFA-1 beta chain in culture did not affect either proliferation or immunoglobulin secretion. The expression of LFA-1 by B cells thus changes as B cells are activated, perhaps reflecting the changing requirements of B cells for interaction with other cells and tissue components. On the other hand, our results did not provide any support for the idea that the LFA-1 antigen is directly involved in the interaction of B cells with lymphokines which control proliferation and differentiation.
Zola
H
H
Department of Clinical Immunology, Flinders Medical Centre, Flinders University of South Australia, Bedford Park.
Furness
V
V
Nikoloutsopoulos
A
A
Neoh
S H
SH
Barclay
S
S
Starr
R
R
Day
A
A
Russ
G R
GR
D000911:Antibodies, Monoclonal; D000917:Antibody Formation; D000943:Antigens, Differentiation / Q000032:analysis*; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D015850:Interleukin-6; D007378:Interleukins / Q000494:pharmacology; D008213:Lymphocyte Activation; D016169:Lymphocyte Function-Associated Antigen-1; D008562:Membrane Glycoproteins / Q000032:analysis*; D014066:Palatine Tonsil / Q000276:immunology; D013050:Spectrometry, Fluorescence
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D015850:Interleukin-6; D007378:Interleukins; D016169:Lymphocyte Function-Associated Antigen-1; D008562:Membrane Glycoproteins