Crude tea extracts decrease the mutagenic activity of N-methyl-N'-nitro-N-nitrosoguanidine in vitro and in intragastric tract of rats.
The effects of tea extracts and their ingredients, catechins and L-ascorbic acid (AsA), on the mutagenicity of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) were examined in vitro and in the stomachs of rats using E. coli WP2 and S. typhimurium TA100. The extracts of green tea and black tea leaves decreased the mutagenic activity of MNNG to E. coli WP2 in vitro in a desmutagenic manner. Catechins such as (-)-epigallocatechin from green tea leaves and the low-molecular-weight tannin fraction isolated from black tea extract with HP-20 resin also exhibited inhibitory effects against the mutagenic activity of MNNG. A desmutagenic effect of AsA on MNNG-induced mutagenicity was observed depending on the dose, though it was complicated. The effects were also demonstrated in the stomachs of rats by assaying the bacterial mutagenic in vitro; the tea extracts previously given orally to rats reduced the mutagenic activity of MNNG remarkably, though simultaneous administration showed less effect. The effectiveness of tea extracts for the decrease of MNNG-induced mutagenesis in vitro and in vivo suggests that the habitual drinking of tea may reduce the tumor-initiating potency of MNNG-type nitrosoureido compounds if they are formed in the stomach.
Jain
A K
AK
Laboratory of Health Science, School of Pharmaceutical Sciences, University of Shizuoka, Japan.
Shimoi
K
K
Nakamura
Y
Y
Kada
T
T
Hara
Y
Y
Tomita
I
I
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D002392:Catechin / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D008769:Methylnitronitrosoguanidine / Q000037:antagonists & inhibitors*; D009152:Mutagenicity Tests; D009154:Mutation / Q000187:drug effects*; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology; D051381:Rats; D012486:Salmonella typhimurium / Q000187:drug effects; D013274:Stomach Neoplasms / Q000139:chemically induced; D013662:Tea
D010936:Plant Extracts; D013662:Tea; D008769:Methylnitronitrosoguanidine; D002392:Catechin; D001205:Ascorbic Acid