Cloning of a thermostable alpha-amylase gene from Thermomonospora curvata and its expression in Streptomyces lividans.
The gene from Thermomonospora curvata CCM 3312 coding for thermostable alpha-amylase (tam) has been cloned in Streptomyces lividans TK 24 and localized to a 2.6 kb HindIII-BamHI fragment of DNA. The data presented here show that the tam gene is expressed at a high level in S. lividans and that the protein is efficiently excreted.
Petrícek
M
M
Department of General Microbiology, Institute of Microbiology, Czechoslovak Academy of Sciences, Prague.
Stajner
K
K
Tichý
P
P
D000191:Actinomycetaceae / Q000201:enzymology*; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D004591:Electrophoresis, Polyacrylamide Gel; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005800:Genes, Fungal; D006358:Hot Temperature; D015183:Restriction Mapping; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics*; D000516:alpha-Amylases / Q000096:biosynthesis / Q000235:genetics*
D004269:DNA, Bacterial; D000516:alpha-Amylases