title: pCloDF13-encoded bacteriocin release proteins with shortened carboxyl-terminal segments are lipid modified and processed and function in release of cloacin DF13 and apparent host cell lysis.
abstract: By oligonucleotide-directed mutagenesis, stop codon mutations were introduced at various sites in the pCloDF13-derived bacteriocin release protein (BRP) structural gene. The expression, lipid modification (incorporation of [3H]palmitate), and processing (in the presence and absence of globomycin) of the various carboxyl-terminal shortened BRPs were analyzed by a special electrophoresis system and immunoblotting with an antiserum raised against a synthetic BRP peptide, and their functioning with respect to release of cloacin DF13, lethality, and apparent host cell lysis were studied in Sup-, supF, and supP strains of Escherichia coli. All mutant BRPs were stably expressed, lipid modified, and processed by signal peptidase II, albeit with different efficiencies. The BRP signal peptide appeared to be extremely stable and accumulated in induced cells. Full induction of the mutant BRPs, including the shortest containing only 4 amino acid residues of the mature polypeptide, resulted in phospholipase A-dependent and Mg2+-suppressible apparent cell lysis. The extent of this lysis varied with the mutant BRP used. Induction of all mutant BRPs also prevented colony formation, which appeared to be phospholipase A independent. One shortened BRP, containing 20 amino acid residues of the mature polypeptide, was still able to bring about the release of cloacin DF13. The results indicated that the 8-amino-acid carboxyl-terminal segment of the BRP contains a strong antigenic determinant and that a small segment between amino acid residues 17 and 21, located in the carboxyl-terminal half of the BRP, is important for release of cloacin DF13. Either the stable signal peptide or the acylated amino-terminal BRP fragments (or both) are involved in host cell lysis and lethality.
authors.affiliation: Department of Molecular Microbiology, Vrije Universiteit, Amsterdam, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: Luirink
authors.forename: D M
authors.initials: DM
authors.lastname: Clark
authors.forename: J
authors.initials: J
authors.lastname: Ras
authors.forename: E J
authors.initials: EJ
authors.lastname: Verschoor
authors.forename: F
authors.initials: F
authors.lastname: Stegehuis
authors.forename: F K
authors.initials: FK
authors.lastname: de Graaf
authors.forename: B
authors.initials: B
authors.lastname: Oudega
mesh_terms: D000215:Acylation; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001430:Bacteriocins / Q000378:metabolism*; D001692:Biological Transport; D002989:Cloacin / Q000378:metabolism*; D004252:DNA Mutational Analysis; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D010455:Peptides / Q000494:pharmacology; D011498:Protein Precursors / Q000378:metabolism; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D021382:Protein Sorting Signals / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001430:Bacteriocins; D029968:Escherichia coli Proteins; D009838:Oligodeoxyribonucleotides; D010455:Peptides; D011498:Protein Precursors; D021382:Protein Sorting Signals; C052368:cloacin DF13-encoded bacteriocin release protein, E coli; D002989:Cloacin; C017339:globomycin