31P NMR spectroscopic study of bioenergetic changes in radiation-induced fibrosarcoma-1 after radiation therapy.
The effects of localized gamma-irradiation on the in vivo 31P NMR spectra of RIF-1 tumors grown subcutaneously in C3H/HeN mice have been examined before and during the week after treatment. Increases in the ratio of phosphocreatine (PCr) to inorganic phosphate (Pi) and in tumor pH, and decreases in the ratio of Pi to the beta phosphorus resonance of the nucleotide triphosphates (beta NTP) were observed in irradiated tumors. The time course of changes in the 31P spectrum following treatment was the opposite of the pattern during untreated growth, and the magnitude and duration of the changes increased with increasing radiation dose, decreasing clonogenic cell survival and increasing growth delay. To examine the possibility that nontherapeutic systemic effects of the tumor irradiation were responsible for the changes observed, a number of animals bearing two tumors were examined. One tumor on each mouse was selectively irradiated. Changes in tumor volume, Pi/beta NTP, PCr/Pi, the ratio of phosphomonoesters to beta NTP, and tumor pH were all significantly different in the treated compared to the untreated tumor on each animal, indicating that these changes in 31P NMR spectra were a response to radiation therapy and not a systemic response to radiation toxicity.
Rajan
S S
SS
Department of Radiology and Radiological Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Wehrle
J P
JP
Li
S J
SJ
Steen
R G
RG
Glickson
J D
JD
D000818:Animals; D002460:Cell Line; D002588:Cesium Radioisotopes / Q000627:therapeutic use; D004734:Energy Metabolism / Q000528:radiation effects; D005260:Female; D005354:Fibrosarcoma / Q000378:metabolism / Q000532:radiotherapy*; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008809:Mice, Inbred C3H; D009368:Neoplasm Transplantation
D002588:Cesium Radioisotopes