title: Use of ZetaPrep cartridge for the purification of human recombinant interleukin 1 beta.
abstract: Zetaprep mass ion-exchange media represent a rapid and efficient chromatographic tool in the separation of proteins, in place of the conventional agarose or cellulose-based gels. We adopted this method, combined with classical steps, to purify to homogeneity human recombinant interleukin 1 beta (IL-1 beta) produced from E. coli and from S. cerevisiae. An anion exchanger QAE-ZetaPrep was used to achieve a rapid partial purification of both proteins. The IL-1 beta purification was completed by gel permeation chromatography on Sephadex G-50. When the protein was produced from yeast, an intermediate chromatographic step on a hydroxylapatite column was also necessary. The isolated proteins proved to be homogeneous by electrophoresis and amino acid analysis. The biological activity of IL-1 beta produced by E. coli is comparable to that of the natural protein, while the protein produced by yeast showed very low specific activity.
authors.affiliation: Sclavo Research Centre, Siena, Italy.
authors.forename: M C
authors.initials: MC
authors.lastname: Casagli
authors.forename: M G
authors.initials: MG
authors.lastname: Borri
authors.forename: C
authors.initials: C
authors.lastname: D'Ettorre
authors.forename: C L
authors.initials: CL
authors.lastname: Galeotti
authors.forename: C
authors.initials: C
authors.lastname: Di Liegro
authors.forename: P
authors.initials: P
authors.lastname: Ghiara
authors.forename: G
authors.initials: G
authors.lastname: Antoni
mesh_terms: D000595:Amino Acid Sequence; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D007375:Interleukin-1 / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae / Q000378:metabolism
chemical_list: D007375:Interleukin-1; D011994:Recombinant Proteins