The SV40 small t antigen is essential for the morphological transformation of human fibroblasts.
The morphological transformation of human fibroblasts as measured in an assay for dense focus formation required, besides the SV40 large T antigen, an intact SV40 small t antigen. Using a G418-resistant colony formation assay it also was found that expression of the SV40 large T antigen only is not sufficient for the morphological transformation of human fibroblasts. Therefore it is concluded that the SV40 small t antigen is essential for the morphological transformation of human fibroblasts.
de Ronde
A
A
Department of Virology, Academic Medical Centre, Amsterdam, The Netherlands.
Sol
C J
CJ
van Strien
A
A
ter Schegget
J
J
van der Noordaa
J
J
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D015153:Blotting, Western; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D006801:Humans; D066298:In Vitro Techniques; D051381:Rats; D014162:Transfection
D000952:Antigens, Polyomavirus Transforming