Deleterious effects of irradiation and bone marrow transplantation therapy in the genetically anemic an/an mouse.
The efficacy and outcome of bone marrow transplantation therapy following lethal irradiation were examined in syngeneic mice that had a hereditary macrocytic anemia (an/an) or were genotypically normal (+/+). Successful RBC and WBC replacement, based on blood cell parameters and donor genetic markers, were observed in all combinations of transplant therapy. Nevertheless, the an/an mice died prematurely several months after treatment, whether they received +/+ or an/an marrow cells. In contrast, the +/+ recipients of either +/+ or an/an marrow cells survived for at least 1 year after transplantation. Premature death of the an/an mice was associated with lymphopenia, anemia, kidney lesions, and severe pathogen-free pneumonitis. On the basis of our results, we hypothesize that the premature deaths of an/an mice are caused by a kind of chronic irradiation damage to which an/an mice are especially susceptible.
Eppig
J T
JT
Jackson Laboratory, Bar Harbor, ME 04609.
Barker
J E
JE
D000740:Anemia / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D000818:Animals; D001853:Bone Marrow / Q000473:pathology / Q000528:radiation effects; D016026:Bone Marrow Transplantation; D017707:Erythrocyte Transfusion; D004912:Erythrocytes / Q000473:pathology / Q000528:radiation effects; D017708:Leukocyte Transfusion; D007962:Leukocytes / Q000473:pathology / Q000528:radiation effects; D008136:Longevity / Q000528:radiation effects; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D011828:Radiation Chimera