Specificity of polymerase chain amplification reactions for human immunodeficiency virus type 1 DNA sequences.
The polymerase chain amplification reaction (PCR) is a sensitive, specific, and quantitative assay of human immunodeficiency virus type 1 (HIV-1). The assay was performed with polymerases from Escherichia coli or Thermus aquaticus (Taq). A single pair of oligonucleotide primers within the long terminal repeat (LTR) sequences were used to detect HIV-1 sequences in infected cell cultures and fresh tissues of the large majority of infected individuals. The amplified product was a faithful copy of this LTR sequence. Utilization of a subsaturating number of cycles of amplification allowed quantitation of HIV-1 DNA sequences.
Bell
J
J
Division of Hematology and Oncology, Washington University, St. Louis, MO 63110.
Ratner
L
L
D001483:Base Sequence; D002478:Cells, Cultured; D004256:DNA Polymerase I; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D013728:Terminator Regions, Genetic; D013824:Thermus / Q000201:enzymology
D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides; D004256:DNA Polymerase I