title: Separation and properties of multiple forms of dihydrodiol dehydrogenase from hamster liver.
abstract: 1. Five multiple forms of dihydrodiol dehydrogenase (EC 1.3.1.20) with similar molecular weights of around 35,000 were purified from hamster liver cytosol. 2. All the enzymes oxidized trans-dihydrodiols of benzene and naphthalene and reduced various carbonyl compounds, but showed clear differences in specificities for other alcohols and cofactors, and in inhibitor sensitivity. 3. Two NADP+-dependent enzymes were immunologically identified with aldehyde reductase (EC 1.1.1.2) and 3 alpha-hydroxytsteroid dehydrogenase (EC 1.1.1.50). 4. The other enzymes with dual cofactor specificity oxidized xenobiotic alicyclic alcohols, and one of them was active on 3 alpha- and 17 beta-hydroxysteroids with NAD+ as a preferable cofactor.
authors.affiliation: Department of Biochemistry, Gifu Pharmaceutical University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Sawada
authors.forename: A
authors.initials: A
authors.lastname: Hara
authors.forename: M
authors.initials: M
authors.lastname: Nakagawa
authors.forename: F
authors.initials: F
authors.lastname: Tsukada
authors.forename: M
authors.initials: M
authors.lastname: Ohmura
authors.forename: K
authors.initials: K
authors.lastname: Matsuura
mesh_terms: D000429:Alcohol Oxidoreductases / Q000302:isolation & purification* / Q000378:metabolism; D000818:Animals; D006224:Cricetinae; D003600:Cytosol / Q000201:enzymology; D007150:Immunohistochemistry; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008297:Male; D008647:Mesocricetus; D008970:Molecular Weight; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; D013379:Substrate Specificity
chemical_list: D009243:NAD; D009249:NADP; D010088:Oxidoreductases; D000429:Alcohol Oxidoreductases; C023125:dihydrodiol dehydrogenases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C116788:cis-1,2-dihydro-1,2-dihydroxynaphthalene dehydrogenase