Beta-carotene stimulates human leukocytes to secrete a novel cytokine.
The effect of beta-carotene on cytokine production by human peripheral blood leukocytes was tested. Beta-carotene stimulated the secretion of a novel cytotoxic cytokine when peripheral blood cells were exposed to carotenoid concentrations between 10(-6) and 10(-10) M. Beta-carotene-treated supernatants caused the cytolysis of four out of the six human tumor cell lines tested. Low level toxicity was also observed when normal diploid fibroblast lines were exposed to beta-carotene-treated leukocyte supernatants. The cytotoxic activity elicited by beta-carotene was found to be distinct from characterized cytokines based on both antisera neutralization and target cell specificity studies. This study demonstrates that beta-carotene can induce human leukocytes to secrete one or more cytokines that can manifest cytotoxic activity against human tumor cells in vitro.
Abril
E R
ER
Department of Internal Medicine, University of Arizona, Tucson 85724.
Rybski
J A
JA
Scuderi
P
P
Watson
R R
RR
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D001685:Biological Factors / Q000378:metabolism*; D002338:Carotenoids / Q000494:pharmacology*; D016207:Cytokines; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D066298:In Vitro Techniques; D007962:Leukocytes / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D011480:Protease Inhibitors / Q000494:pharmacology; D012176:Retinoids / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D019207:beta Carotene
D000276:Adjuvants, Immunologic; D000970:Antineoplastic Agents; D001685:Biological Factors; D016207:Cytokines; D007106:Immune Sera; D011480:Protease Inhibitors; D012176:Retinoids; D014409:Tumor Necrosis Factor-alpha; D019207:beta Carotene; D002338:Carotenoids