title: Effects of long-term hypergastrinaemia on the ultrastructure of enterochromaffin-like cells in the stomach of the rat, hamster and guinea pig.
abstract: The present report describes the ultrastructure of the enterochromaffin-like (ECL) cells in the stomach of the rat, hamster and guinea pig, and the ultrastructural consequences of long-term hypergastrinaemia evoked either by continuous infusion of synthetic human (Leu15)-gastrin-17 for 4 weeks (rats) or by daily treatment with large doses of the antisecretory agent omeprazole for 2-10 weeks (rats, hamsters and guinea pigs). As a result, the ECL cells increased greatly in size (maximal effect after 2 weeks of omeprazole treatment, no further gain in size after 4 or 10 weeks). Also the endoplasmic reticulum and Golgi area were enlarged. The most conspicuous feature of the ECL cells is the cytoplasmic vesicles, which are of varying size and either devoid of a dense core or with a small, often eccentrically located dense core. The vesicles probably represent the main storage site of the secretory products of the ECL cell. In addition, the cytoplasm contains granules, which differ from the vesicles in that they possess a more or less electron-dense core, surrounded by a narrow halo. The size of the vesicles ranged from small to very large, while the granules were uniformly small. Many vesicles were seen to lie very close together, some displaying an irregular outline (vacuole-like vesicles), at times giving the impression that they were undergoing fusion. The profile size (median value) of the vesicles was unaffected by gastrin infusion for 4 weeks. However, there was a tendency to a relative increase in the number of very small vesicles. In contrast, the vesicles became larger during the omeprazole treatment. Also, the number of vesicles that seemed to be engaged in fusion increased after omeprazole treatment but not after gastrin infusion. The observations support the view that ECL cells are influenced by gastrin. The effects of gastrin infusion and of omeprazole treatment on ECL cell ultrastructure were not completely identical. It cannot be excluded that the omeprazole-evoked achlorhydria evokes effects unrelated to those of hypergastrinaemia on the ECL cells, or that endogenous gastrins may evoke effects that are in some ways distinct from those of synthetic human (Leu15)-gastrin-17. Alternatively, the additional effects seen after long-term omeprazole treatment may reflect simply the duration of the hypergastrinaemic stimulus.
authors.affiliation: Department of Medical Cell Research, University of Lund, Sweden.
authors.forename: G
authors.initials: G
authors.lastname: Böttcher
authors.forename: R
authors.initials: R
authors.lastname: Håkanson
authors.forename: G
authors.initials: G
authors.lastname: Nilsson
authors.forename: R
authors.initials: R
authors.lastname: Seensalu
authors.forename: F
authors.initials: F
authors.lastname: Sundler
mesh_terms: D000818:Animals; D002838:Chromaffin System / Q000187:drug effects*; D006224:Cricetinae; D004759:Enterochromaffin Cells / Q000187:drug effects* / Q000648:ultrastructure; D005260:Female; D005755:Gastrins / Q000008:administration & dosage*; D006168:Guinea Pigs; D008297:Male; D008647:Mesocricetus; D008854:Microscopy, Electron; D009853:Omeprazole / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013270:Stomach / Q000166:cytology* / Q000187:drug effects; D013997:Time Factors
chemical_list: D005755:Gastrins; D009853:Omeprazole