Characterization of the immunodeficiency of RIIS/J [corrected] mice. I. Association with the CD5 (LY-1) [corrected] B cell lineage.
RIIIS/J mice produce low antibody responses to several polysaccharide Ag of bacterial origin. They have low levels of serum IgM and IgG3 and high levels of serum IgG2a and IgG2b. Low serum IgM and IgG3 have been attributed to a low frequency of CD5 (Ly-1) B cells, which play an important role in the production of natural antibodies. Indeed, RIIIS/J mice have a low frequency of CD5 (Ly-1)+, IgM bright+, Ly-5 (B220)dull+ (i.e., CD5 (Ly-1) B) cells in their peritoneum. RIIIS/J mice treated with LPS produce a low anti-bromelain-treated mouse RBC splenic plaque-forming cell response and a normal anti-mouse transferrin splenic PFC response. Those data are compatible with the fact that CD5 (Ly-1) B cells contain the precursors of B lymphocytes secreting anti-bromelain-treated mouse RBC antibody. However, they have a higher frequency of IgM bright+, Mac-1+ cells in their peritoneum. These cells represent the CD5 (Ly-1) "sister population" of CD5 (Ly-1) B cells described by others. This suggests that characteristics usually associated with the CD5 (Ly-1) lineage are applicable only to the CD5 (Ly-1)+ Mac-1+ IgM+ population, but not the related CD5 (Ly-1)- Mac-1+ IgM+ population. RIIIS/J mice should thus prove a valuable model to study the CD5 (Ly-1) B cell lineage.
Hiernaux
J R
JR
Laboratory of Microbial Immunity, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892.
Goidl
E A
EA
McEvoy
S J
SJ
Stashak
P W
PW
Baker
P J
PJ
Holmes
K L
KL
D000818:Animals; D000950:Antigens, Ly / Q000276:immunology*; D001323:Autoantibodies / Q000096:biosynthesis; D001324:Autoantigens / Q000276:immunology; D001402:B-Lymphocytes / Q000145:classification / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006462:Hemolytic Plaque Technique; D007132:Immunoglobulin Isotypes / Q000378:metabolism; D007153:Immunologic Deficiency Syndromes / Q000097:blood / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008814:Mice, Inbred NZB; D010641:Phenotype; D013154:Spleen
D000950:Antigens, Ly; D001323:Autoantibodies; D001324:Autoantigens; D007132:Immunoglobulin Isotypes