An improved method for the isolation of enriched canine peripheral blood mononuclear cell and peripheral blood lymphocyte preparations.
A two-step purification method was developed for obtaining (1) peripheral blood mononuclear cell preparations of greater than 97% purity and (2) peripheral blood lymphocyte preparations of greater than 95% purity from canine whole blood with yields similar to or greater than those obtained by conventional techniques.
Wunderli
P S
PS
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Illinois, Urbana 61801.
Felsburg
P J
PJ
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D002499:Centrifugation, Density Gradient; D004285:Dogs / Q000097:blood*; D006462:Hemolytic Plaque Technique; D007958:Leukocyte Count / Q000662:veterinary; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000166:cytology*