Immunohistochemical analysis of non-Hodgkin's lymphomas with the monoclonal antibody to follicular center lymphocytes reactive in paraffin sections.
The monoclonal antibody designated LN-1 was used in an attempt to identify the antigen in follicular center cell lymphomas using tissue sections fixed in formalin. The LN-1 antibody has been shown in previous studies to identify follicular center cells and give reproducible results in tissue fixed in B5. We used the ABC peroxidase technique to examined formalin-fixed, paraffin embedded sections representing 52 cases of various histologic subgroups of non-Hodgkin's lymphomas based upon the Lukes-Collins classification. Following immunostaining with LN-1 using overnight incubation of the antibody and papain treated sections, 37 cases, and all of the 38 cases previously diagnosed as follicular center cell lymphomas, gave a positive reaction to the LN-1 monoclonal antibody.
Bhoopat
L
L
Thamprasert
K
K
Watanabooyakhet
P
P
Epstein
A
A
D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000276:immunology*; D002455:Cell Division; D006801:Humans; D007124:Immunoenzyme Techniques; D008228:Lymphoma, Non-Hodgkin / Q000276:immunology*; D010232:Paraffin
D000911:Antibodies, Monoclonal; D010232:Paraffin