Crystallization and preliminary X-ray analysis of the human c-H-ras-oncogene product p21 complexed with GTP analogues.
The catalytic domain (amino acid residues 1 to 166) of the human ras-oncogene product p21 complexed with the GTP analogues beta,gamma-imido-GTP (GMPPNP), beta,gamma-methylene-GTP (GMPPCP), and guanosine-5'-(gamma-thiotriphosphate) (GTP gamma S) have been been crystallized. Crystals of the GMPPNP and GMPPCP complexes are well suited for high resolution X-ray crystallography. They belong to space group P3(1)21 (or its enantiomorph P3(2)21) with unit cell axes a=b=40.3 A and c = 162.2 A.
Scherer
A
A
Biophysik, Max-Planck-Institut für Medizinische Forschung, Heidelberg, F.R.G.
John
J
J
Linke
R
R
Goody
R S
RS
Wittinghofer
A
A
Pai
E F
EF
Homes
K C
KC
D001483:Base Sequence; D003460:Crystallization; D011905:Genes, ras; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives*; D006801:Humans; D046911:Macromolecular Substances; D014961:X-Ray Diffraction
D046911:Macromolecular Substances; D006160:Guanosine Triphosphate