Removal of background label in immunocytochemistry with the apolar Lowicryls by using washed protein A-gold-precoupled antibodies in a one-step procedure.
Antiserum mixed with protein A-gold produces aggregates that can be washed by centrifugation and redissociated by ultrasonication. These precoupled antibodies are used for immunocytochemical labeling in a one-step procedure and reveal a very low background on sections of material embedded in polar and apolar Lowicryls. Controls have shown that the background label stems from serum components with only one protein A binding site and is discarded during the wash steps of the precoupling procedure.
Dürrenberger
M B
MB
Biocenter, University of Basel, Switzerland.
D000180:Acrylic Resins; D001426:Bacterial Proteins / Q000276:immunology*; D004926:Escherichia coli / Q000378:metabolism / Q000648:ultrastructure; D006046:Gold; D007150:Immunohistochemistry / Q000379:methods*; D008854:Microscopy, Electron; D013205:Staphylococcal Protein A
D000180:Acrylic Resins; D001426:Bacterial Proteins; D013205:Staphylococcal Protein A; D006046:Gold