Evaluation of prostacyclin production by human gallbladder.
The prostanoids have been demonstrated to be involved in gallbladder physiology and disease. In previous reports, prostaglandin E (PGE) compounds were found to be increased in inflamed human gallbladders. Prostaglandin synthetase inhibition decreased PGE formation by human gallbladders; however, the relief of symptoms of cholecystitis did not correlate well with the decrease in PGE formation. This suggested that other prostanoids may be involved in cholecystitis. The purpose of this study was to evaluate the production of the proinflammatory arachidonic acid metabolite prostacyclin by gallbladders from patients with calculous cholecystitis. The formation of PGE and 6-ketoprostaglandin F1 alpha (6-keto-PGF1 alpha), the stable metabolite of prostacyclin, in normal human gallbladder mucosal cells and muscle tissue was compared with that produced by diseased mucosal cells and muscle tissue. Normal human gallbladders produced small amounts of 6-keto-PGF1 alpha, and no differences in formation rates were evident when muscle tissue was compared with mucosal cells. Diseased gallbladders produced significantly greater amounts of 6-keto-PGF1 alpha than did normal gallbladders, and diseased gallbladder muscle produced approximately four times greater amounts of 6-keto-PGF1 alpha than did diseased gallbladder mucosa. Prostacyclin formation is increased in diseased human gallbladders and may be an important mediator of the inflammatory changes of cholecystitis.
Kaminski
D L
DL
Department of Surgery, St Louis University Medical Center 63104.
Deshpande
Y G
YG
Westfall
S
S
Herbold
D
D
D000208:Acute Disease; D002764:Cholecystitis / Q000378:metabolism*; D002769:Cholelithiasis / Q000378:metabolism; D002908:Chronic Disease; D011464:Epoprostenol / Q000096:biosynthesis*; D005260:Female; D005704:Gallbladder / Q000378:metabolism*; D005966:Glucuronidase / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D009092:Mucous Membrane / Q000378:metabolism; D011460:Prostaglandins F / Q000096:biosynthesis; D012016:Reference Values
D011460:Prostaglandins F; D011464:Epoprostenol; D005966:Glucuronidase; C100006:prostaglandin F1