Islet amyloid polypeptide inhibits glucose-stimulated insulin secretion from isolated rat pancreatic islets.
Islet amyloid polypeptide has 37 amino acids and is a major component of amyloid deposition in pancreatic islets of patients with type 2 diabetes mellitus. To determine whether the peptide is involved in the impaired insulin secretion in this type of diabetes mellitus, we synthesized islet amyloid polypeptide and its fragments and examined its effect on insulin secretion. Islet amyloid polypeptide inhibited the glucose-stimulated insulin secretion from isolated rat pancreatic islets, as calcitonin gene-related peptide did, but the fragments failed to inhibit the secretion. Thus, we propose that amyloid deposition may be an important factor in the impairment of insulin secretion in type 2 diabetes mellitus.
Ohsawa
H
H
Second Department of Internal Medicine, Chiba University, School of Medicine, Japan.
Kanatsuka
A
A
Yamaguchi
T
T
Makino
H
H
Yoshida
S
S
D000596:Amino Acids / Q000302:isolation & purification; D000682:Amyloid / Q000138:chemical synthesis / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D015740:Calcitonin Gene-Related Peptide; D002469:Cell Separation; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism / Q000503:physiopathology; D005947:Glucose; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D058228:Islet Amyloid Polypeptide; D007515:Islets of Langerhans / Q000378:metabolism / Q000502:physiology*; D008297:Male; D009479:Neuropeptides / Q000494:pharmacology; D010446:Peptide Fragments / Q000302:isolation & purification / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D000596:Amino Acids; D000682:Amyloid; D007328:Insulin; D058228:Islet Amyloid Polypeptide; D009479:Neuropeptides; D010446:Peptide Fragments; D005947:Glucose; D015740:Calcitonin Gene-Related Peptide