The insulin-mimetic effect of vanadate is not correlated with insulin receptor tyrosine kinase activity nor phosphorylation in mouse diaphragm in vivo.
The in vivo administration of sodium orthovanadate stimulated the incorporation of [14C]glucose into [14C] glycogen, in a dose- and time-dependent manner, in mouse diaphragm. Activation of diaphragm insulin receptor was measured by exogenous tyrosine kinase activity and an antibody that recognizes a conformational change in the receptor beta-subunit upon autophosphorylation. Neither method detected insulin receptor activation by in vivo vanadate administration, suggesting that vanadate's insulin-mimetic effect on mouse diaphragm glycogenesis occurs at a site distal to the insulin receptor.
Strout
H V
HV
Department of Biochemical Endocrinology, Merck, Sharp, and Dohme Research Laboratories, Rahway, New Jerey 07065.
Vicario
P P
PP
Saperstein
R
R
Slater
E E
EE
D000818:Animals; D003964:Diaphragm; D006003:Glycogen / Q000096:biosynthesis; D007328:Insulin / Q000502:physiology*; D051379:Mice; D009132:Muscles / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D010766:Phosphorylation; D011233:Precipitin Tests; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D011972:Receptor, Insulin / Q000378:metabolism*; D014638:Vanadates / Q000494:pharmacology*
D007328:Insulin; D014638:Vanadates; D006003:Glycogen; D011505:Protein-Tyrosine Kinases; D011972:Receptor, Insulin