title: Ferritin subunits in livers of siderotic mice.
abstract: The major ferritin species of mouse liver has been resolved by SDS-PAGE into two bands similar to the H and L subunits of rat liver ferritin with the L subunit predominating. Amino acid sequencing has confirmed the major, faster-migrating component as L chain. An additional, electrophoretically fast, minor ferritin was isolated from siderosome-containing subcellular fractions. In denaturing gels it gave a single 'F' subunit band of about 17 kDa, significantly smaller than the L and H subunits (about 20 and 21 kDa respectively). A small fragment isolated from the fast ferritin was sequenced. It corresponds to a 19-residue C-terminal peptide cleaved from L subunits in the assembled molecules. The F subunit must be derived from L subunits by loss of this peptide, and is not the expression product of a different gene. 'Fast' ferritins of siderotic mice and rats are thus analogous.
authors.affiliation: Department of Molecular Biology and Biotechnology, University, Sheffield, UK.
authors.forename: B
authors.initials: B
authors.lastname: Dean
authors.forename: S C
authors.initials: SC
authors.lastname: Andrews
authors.forename: A
authors.initials: A
authors.lastname: Treffry
authors.forename: P M
authors.initials: PM
authors.lastname: Harrison
authors.forename: J N
authors.initials: JN
authors.lastname: Keen
authors.forename: J B
authors.initials: JB
authors.lastname: Findlay
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001052:Apoferritins / Q000302:isolation & purification; D003488:Cyanogen Bromide; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005293:Ferritins / Q000302:isolation & purification / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D012806:Siderosis / Q000378:metabolism*
chemical_list: D005293:Ferritins; D001052:Apoferritins; D003488:Cyanogen Bromide