[Effect of bacterial toxins on the mitogen-induced increase of the Ca2+ concentration in the cytoplasm of rat thymocytes. The role of N proteins].
The effect of bacterial toxins, modifying the activity of regulatory N proteins of adenylate cyclase and probably other systems, on the mitogen-induced changes of cytosolic free Ca2+ concentration ([Ca2+]i) has been studied using Ca2+ fluorescent probe quin-2. It is shown that treatment of thymocytes with cholera toxin, E. coli heat-labile (HL) toxin or pertussis toxin abolishes the concanavalin A (con A)-induced rise of [Ca2+]i. The inhibitory effect of cholera and HL toxins can be explained by the toxin-induced rise of intracellular cAMP. The effect of pertussis toxin indicates the involvement of N proteins in the action of con A receptor and in generation of Ca2+-signal during the mitogenic activation of thymocytes.
Gukovskaia
A S
AS
Astashkin
E I
EI
Gureeva
A A
AA
Nikolaeva
I S
IS
Surin
A M
AM
D037361:Adenylate Cyclase Toxin; D000818:Animals; D001427:Bacterial Toxins / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003593:Cytoplasm / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004768:Enterotoxins / Q000494:pharmacology; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D008934:Mitogens / Q000494:pharmacology*; D037342:Pertussis Toxin; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D014734:Vibrio cholerae; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D037361:Adenylate Cyclase Toxin; D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D008934:Mitogens; D011506:Proteins; D010566:Virulence Factors, Bordetella; C037420:stN protein, Vibrio cholerae; D003208:Concanavalin A; C027538:heat-labile enterotoxin, E coli; D037342:Pertussis Toxin; D002118:Calcium
Vliianie bakterial'nykh toksinov na indutsirovannoe mitogenom povyshenie kontsentratsii Ca2+ v tsitoplazme timotsitov krysy. Rol' N-belkov.