Fluorescence reaction of chromatin by curcumin.
Treatment of cell smears, paraffin, and Epon tissue sections with aqueous solutions of curcumin results in a green fluorescence reaction in chromatin under violet-blue excitation which is abolished after extraction procedures with DNase and TCA. The selective fluorescence characteristics of curcumin support the possibility of employing this dye as a new fluorochrome.
Stockert
J C
JC
Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.
Del Castillo
P
P
Gómez
A
A
Llorente
A R
AR
D000818:Animals; D002645:Chickens; D002843:Chromatin / Q000648:ultrastructure*; D003474:Curcumin; D004912:Erythrocytes / Q000166:cytology*; D005456:Fluorescent Dyes; D008856:Microscopy, Fluorescence / Q000379:methods; D013050:Spectrometry, Fluorescence; D013154:Spleen / Q000166:cytology; D014349:Trypanosoma cruzi / Q000166:cytology
D002843:Chromatin; D005456:Fluorescent Dyes; D003474:Curcumin