Alpha-agglutinin expression in Saccharomyces cerevisiae.
A polyclonal antiserum raised against purified alpha-agglutinin was made specific for alpha-agglutinin after adsorption with a cells. The adsorbed antiserum identified alpha-agglutinin peptides on Western blots and bound to cell surface alpha-agglutinin, inhibiting the binding of alpha cells to a cells. Using the antibody, we have determined that 1) the surface distribution of alpha-agglutinin on alpha cells is polar, 2) about 5 x 10(4) molecules/cell are constitutively expressed on strain X2180-1B (alpha) cells, and 3) treatment of alpha cells with the sex pheromone a-factor causes an increase in cell surface alpha-agglutinin, consistent with the a-factor induced increase in cell agglutinability.
Wojciechowicz
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Department of Biological Sciences, Hunter College, New York 10021.
Lipke
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PN
D000373:Agglutinins / Q000032:analysis* / Q000276:immunology; D000908:Antibodies, Fungal; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D005656:Fungal Proteins / Q000032:analysis* / Q000276:immunology; D000072235:Mating Factor; D008562:Membrane Glycoproteins / Q000032:analysis / Q000276:immunology; D010455:Peptides / Q000032:analysis* / Q000276:immunology; D012441:Saccharomyces cerevisiae / Q000032:analysis*
D000373:Agglutinins; D000908:Antibodies, Fungal; D005656:Fungal Proteins; D008562:Membrane Glycoproteins; D010455:Peptides; D000072235:Mating Factor