Dexamethasone regulation of the expression of cytokine mRNAs induced by interleukin-1 in the astrocytoma cell line U373MG.
BSF-2/IL-6, GM-CSF and IL-1 beta mRNAs were induced by recombinant IL-1 in human astrocytoma cell line U373MG. The induction of BSF-2/IL-6 and IL-1 beta mRNAs did not require de novo protein synthesis while that of GM-CSF mRNA required a newly synthesized protein. Dexamethasone inhibited the induction of these cytokine mRNAs by IL-1. This process seems to require continued protein synthesis. These results suggest that the production of these cytokines are positively and negatively controlled by IL-1 and glucocorticoids, respectively, in astrocytes.
Nishida
T
T
Cellular Technology Institute, Otsuka Pharmaceutical Co., Tokushima, Japan.
Nakai
S
S
Kawakami
T
T
Aihara
K
K
Nishino
N
N
Hirai
Y
Y
D001254:Astrocytoma; D002460:Cell Line; D003115:Colony-Stimulating Factors / Q000235:genetics*; D003513:Cycloheximide / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D005796:Genes / Q000187:drug effects*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000235:genetics*; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics / Q000502:physiology*; D015850:Interleukin-6; D007378:Interleukins / Q000235:genetics*; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics*; D011994:Recombinant Proteins / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects*
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D007375:Interleukin-1; D015850:Interleukin-6; D007378:Interleukins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D003907:Dexamethasone; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D003513:Cycloheximide