Characterization of a halothane-resistant strain of measles virus.
A strain of measles virus (MVr) whose replication demonstrated increased resistance to halothane (2-bromo-2-chloro-1,1,1-trifluoroethane) exposure compared with the susceptible parental strain (MVs) is described. After exposure to a 1.2% halothane concentration, substantial amounts of the measles virus H protein were detected in MVr-infected Vero cell lysates by immunoprecipitation and polyacrylamide gel electrophoresis or by quantitative immunofluorescence staining. The protein was barely detectable in identically treated MVs-infected lysates, however. The recovery of all other measles virus proteins studied was the same in MVr- and MVs-infected cells at this anesthetic concentration. Thus, the altered expression of a single gene product appears to be responsible for the observed halothane resistance.
Bedows
E
E
Department of Anesthesiology, University of Michigan Medical Center, Ann Arbor 48109.
Davidson
B A
BA
Williams
B A
BA
Knight
P R
PR
D000818:Animals; D004352:Drug Resistance, Microbial; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D006221:Halothane / Q000494:pharmacology*; D008459:Measles virus / Q000032:analysis / Q000187:drug effects*; D014709:Vero Cells; D014764:Viral Proteins / Q000032:analysis
D014764:Viral Proteins; D006221:Halothane