Influence of bacterial growth of the overnight culture on the captan- and folpet-induced reversion in the Ames test.
The influence of the stage of bacterial growth of the overnight culture on captan- and folpet-induced reversion in the Ames test was investigated. Tests were performed in order to determine both the number of viable bacteria at different intervals of bacterial growth--in the presence or absence of captan and folpet--and also the number of revertants. Captan or folpet were tested at 5 micrograms per plate, a concentration which had previously showed a mutagenic effect to the TA100 strain. The highest induced reversion was obtained when the bacterial culture used had entered the stationary-phase after 10-14 h, with a titre of at least 10(8) viable cells per ml.
Barrueco
C
C
Instituto de Edafología y Biología Vegetal, Madrid, Spain.
de la Peña
E
E
D001419:Bacteria / Q000187:drug effects / Q000254:growth & development*; D002215:Captan / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D015169:Colony Count, Microbial; D004305:Dose-Response Relationship, Drug; D009152:Mutagenicity Tests; D009154:Mutation; D010797:Phthalimides / Q000633:toxicity*
D010797:Phthalimides; D002215:Captan; C005020:folpet