Immunoelectron microscopic localization of fibrin-related antigen in human glomerular diseases.
The distribution of fibrin-related antigen (FRA) in glomeruli was examined by immunoelectron microscopy in 9 patients with idiopathic membranous nephropathy (MN), 8 patients with minimal-change nephrotic syndrome, and 10 patients with IgA nephropathy (IgA-N), using antisera against human gamma--chain, alpha-chain, mu-chain, and fibrinogen. Electron-dense reaction products of FRA were observed in the endothelium, subendothelium, and/or in electron-dense deposits (EDD). Among the three glomerular diseases, the amount of electron-dense reaction products of FRA in the endothelium was highest in MN. This suggests that coagulation occurs on the endothelium in MN. Although the mesangial EDD of IgA-N were intensely stained with reaction products of FRA, the staining was weak in the subepithelial EDD of MN. This suggests that FRA hardly penetrates into the subepithelial EDD in MN.
Ono
T
T
Department of Internal Medicine, Amagasaki Hospital, Japan.
Kanatsu
K
K
Doi
T
T
Sekita
K
K
Nagai
H
H
Muso
E
E
Yoshida
H
H
Tamura
T
T
Kawai
C
C
D000941:Antigens; D005337:Fibrin / Q000276:immunology / Q000648:ultrastructure; D005922:Glomerulonephritis, IGA / Q000473:pathology*; D015433:Glomerulonephritis, Membranous / Q000473:pathology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007678:Kidney Glomerulus / Q000648:ultrastructure; D009402:Nephrosis, Lipoid / Q000473:pathology*
D000941:Antigens; D005337:Fibrin