title: The development of an immunosensor for the electrochemical determination of the isoenzyme LDH5.
abstract: In this work we describe an immunoelectrochemical technique for the determination of LDH isoenzymes. The method is based on the covalent binding of monoclonal antibody against the isoenzyme LDH5 to a glassy carbon electrode, and the exploitation of the electrode surface as the solid phase for enzyme immunoassay. The resulting enzyme electrode was studied by a computerized electrochemical system. This combination formed the basis of a highly sensitive fast and convenient technique for the determination of the LDH isoenzymes in sera.
authors.affiliation: Department of Biotechnology, Faculty of Life science, Tel-Aviv University, Ramat Aviv, Israel.
authors.forename: J
authors.initials: J
authors.lastname: Rishpon
authors.forename: I
authors.initials: I
authors.lastname: Rosen
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D015374:Biosensing Techniques; D004563:Electrochemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000032:analysis* / Q000097:blood / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase