Comparison of three methods for detection of Cryptosporidium oocysts in a low-prevalence population.
Four hundred fecal specimens which had been received for routine ova and parasite examination were concentrated by Formalin-ether sedimentation. Sediments were examined as saline and iodine-stained wet preparations and were stained with rhodamine-auramine O and a commercially available monoclonal fluorescent-antibody stain for oocysts of Cryptosporidium species. Examination with the fluorescent stains detected cryptosporidia in both positive specimens (0.5% prevalence), and routine direct wet-preparation examination detected cryptosporidia in one of them. Detection of only low numbers of positive specimens in our nonrisk population argues against routine use of specific and expensive stain reagents.
Baron
E J
EJ
North Shore University Hospital, Manhasset, New York 11030.
Schenone
C
C
Tanenbaum
B
B
D000818:Animals; D001576:Benzophenoneidum; D003044:Coccidia / Q000302:isolation & purification*; D003457:Cryptosporidiosis / Q000175:diagnosis / Q000453:epidemiology*; D003458:Cryptosporidium / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D012235:Rhodamines; D012394:Rosaniline Dyes; D012621:Seasons
D012235:Rhodamines; D012394:Rosaniline Dyes; D001576:Benzophenoneidum; C006898:carbol-fuchsin solution