A mutational analysis of the bacteriophage P1 cin recombinase gene: intragenic complementation.
Bacteriophage P1 encodes a site-specific recombinase, Cin, which regulates the alternate expression of tail fibre genes by inverting a DNA segment. To define regions of Cin important for the recombination process, we have isolated and characterised 24 different mutations of the cin gene. Most of these mutations affected amino acids that are highly conserved in other related recombinases. Some of these mutants complement each other in vivo. This intragenic complementation could be due to the assembly of heteromers containing both mutant proteins, suggesting that the active enzyme is at least a dimer.
Haffter
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P
Department of Microbiology, Biozentrum, Basel University, Switzerland.
Pripfl
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Bickle
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TA
D000595:Amino Acid Sequence; D001483:Base Sequence; D003062:Codon / Q000235:genetics; D003090:Coliphages / Q000201:enzymology / Q000235:genetics*; D004254:DNA Nucleotidyltransferases / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D005814:Genes, Viral; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009154:Mutation
D003062:Codon; D004254:DNA Nucleotidyltransferases; C039930:cin recombinase