title: Re-evaluation of the phagocytic respiratory burst in the physiological or inflammatory state and in aging.
abstract: We studied the differences in oxygen metabolite generation, using a chemiluminescence (CL) assay, in peripheral blood phagocytic cells from various donors including healthy young volunteers, patients with acute or chronic inflammation, pregnant women, and elderly persons. The CL response of polymorphonuclear leukocytes (PMN) after stimulation with serum-opsonized zymosan was increased in patients with acute inflammation due to infection and in pregnant women as compared with that in controls. Monocytes from those patients also showed a slight increase of the CL response. In contrast, CL of monocytes from patients with chronic inflammation (Crohn's disease patients) and elderly persons was significantly enhanced, whereas that of their PMN remained in the range of control values. The significance of these results was discussed.
authors.affiliation: Department of Microbiology, Fukuoka University School of Medicine, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Kuroiwa
authors.forename: K
authors.initials: K
authors.lastname: Miyamoto
authors.forename: N
authors.initials: N
authors.lastname: Okabe
authors.forename: T
authors.initials: T
authors.lastname: Shibuya
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000097:blood*; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood*; D008163:Luminescent Measurements; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism; D010100:Oxygen / Q000097:blood*; D010586:Phagocytes / Q000378:metabolism*; D011247:Pregnancy / Q000097:blood*
chemical_list: D010100:Oxygen