Low cost apparatus for primer-directed DNA amplification using Thermus aquaticus-DNA polymerase.
An apparatus is described which permits the incubation of samples at three different temperatures in a cyclic fashion. The parts for the incubator are either present in every biochemical laboratory (water baths) or can be easily obtained at a low price (timers and magnetic valves). Thus the new DNA amplification procedure employing Thermus aquaticus-DNA polymerase can be carried out automatically without major investments.
Torgersen
H
H
Institut fuer Biochemie, Vienna, Austria.
Blaas
D
D
Skern
T
T
D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D005784:Gene Amplification; D005821:Genetic Techniques / Q000295:instrumentation; D007700:Kinetics; D019914:Taq Polymerase; D013816:Thermodynamics; D013824:Thermus / Q000201:enzymology*
D019914:Taq Polymerase; D004259:DNA-Directed DNA Polymerase