Chromosomal transfer and in vivo cloning of genes in Vibrio cholerae using RP4::mini-Mu.
The RP4::mini-Mu replicon has been used to transfer chromosomal genes by conjugation and to clone in vivo metabolic, toxin and flagellar genes of Vibrio cholerae. RP4::mini-Mu was introduced into several strains of V. cholerae and these strains were mated with V. cholerae or Escherichia coli K-12 recipients. R'-episomes carrying the respective genes were maintained in recA recipients and were detected by complementation of auxotrophic, nontoxinogenic and aflagellate mutations in V. cholerae.
Srivastava
R
R
Division of Microbial Genetics, Central Drug Research Institute, Lucknow, India.
Sinha
V B
VB
Srivastava
B S
BS
D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000235:genetics; D004768:Enterotoxins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005407:Flagella / Q000648:ultrastructure; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D005822:Genetic Vectors; D009154:Mutation; D011815:R Factors; D012093:Replicon; D014734:Vibrio cholerae / Q000235:genetics* / Q000254:growth & development
D004269:DNA, Bacterial; D004768:Enterotoxins; C037420:stN protein, Vibrio cholerae