Construction and shuttling of novel bifunctional vectors for Streptomyces spp. and Escherichia coli.
Shuttle vectors for gene transfer between Streptomyces spp. and Escherichia coli have been constructed by fusion of an artificial multicopy E. coli replicon and DNA fragments of pIJ702. Stable transfer to Streptomyces lividans was obtained. Marked differences in transformation efficiency were observed when plasmid DNA isolated from E. coli GM119 was used instead of that from strain HB101.
Neesen
K
K
Division of Genetics, Faculty of Agricultural Sciences, University of Leuven, Belgium.
Volckaert
G
G
D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D013302:Streptomyces / Q000235:genetics*; D014169:Transformation, Bacterial