Techniques in immunocytochemistry. Application to diagnostic pathology.
The techniques used in the localization of antigens in histologic tissues are reviewed. The principles and conditions for successful antibody-antigen binding are presented. The importance of fixation is stressed. The advantages and disadvantages of monoclonal antibodies over polyclonal antisera are described. Applications of immunocytochemical techniques in diagnostic surgical pathology and cytology are discussed.
Pettigrew
N M
NM
Department of Pathology, University of Manitoba, Winnipeg, Canada.
D000906:Antibodies / Q000032:analysis / Q000276:immunology; D000918:Antibody Specificity; D005404:Fixatives; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D010338:Pathology, Clinical
D000906:Antibodies; D005404:Fixatives