title: Synthesis of the vasoconstrictor peptide endothelin in kidney cells.
abstract: The expression plasmid containing human prepro-endothelin cDNA was constructed and introduced into COS-7 cells. Mature endthelin, consisting of 21 amino acid residues, was secreted into the culture medium of the transfected cells and was also synthesized by non-transfected COS-7 cells. Normal kidney cells derived from other species also synthesized and secreted endothelin. Partial characterization of endothelins produced by kidney cells suggested that existence of new types of endothelin. This is the first report of the vasoconstrictor peptide endothelin being synthesized in kidney cells.
authors.affiliation: Tsukuba Research Laboratories, Takeda Chemical Industries Ltd., Ibaraki, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kosaka
authors.forename: N
authors.initials: N
authors.lastname: Suzuki
authors.forename: H
authors.initials: H
authors.lastname: Matsumoto
authors.forename: Y
authors.initials: Y
authors.lastname: Itoh
authors.forename: T
authors.initials: T
authors.lastname: Yasuhara
authors.forename: H
authors.initials: H
authors.lastname: Onda
authors.forename: M
authors.initials: M
authors.lastname: Fujino
mesh_terms: D000818:Animals; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000032:analysis; D004247:DNA / Q000032:analysis; D016232:Endothelins; D006801:Humans; D007124:Immunoenzyme Techniques; D007668:Kidney / Q000378:metabolism*; D010452:Peptide Biosynthesis; D010455:Peptides / Q000235:genetics; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis*; D013045:Species Specificity; D014162:Transfection
chemical_list: D003470:Culture Media; D016232:Endothelins; D010455:Peptides; D011994:Recombinant Proteins; D004247:DNA