title: Lipopolysaccharide-induced insulin resistance in monolayers of cultured hepatocytes.
abstract: In order to clarify the endotoxin effect on the hepatic removal of insulin, the influence of lipopolysaccharide (LPS) from E. coli 0111:B4 on the insulin binding and endocytosis in cultured hepatocytes from adult male rats has been investigated. LPS decreases both processes in a time and temperature-dependent manner, showing a major effect at short time and low temperature, according to the characteristics of LPS binding and uptake.
authors.affiliation: Department of Biochemistry & Molecular Biology I, Faculty of Chemistry, Universidad Complutense, Madrid, Spain.
authors.forename: M T
authors.initials: MT
authors.lastname: Portolés
authors.forename: R
authors.initials: R
authors.lastname: Pagani
authors.forename: M J
authors.initials: MJ
authors.lastname: Ainaga
authors.forename: I
authors.initials: I
authors.lastname: Díaz-Laviada
authors.forename: A M
authors.initials: AM
authors.lastname: Municio
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003080:Cold Temperature; D004926:Escherichia coli; D007333:Insulin Resistance; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008099:Liver / Q000166:cytology / Q000187:drug effects*; D008297:Male; D051381:Rats; D013997:Time Factors
chemical_list: D008070:Lipopolysaccharides