Biosynthesis and secretion of thrombospondin in human melanoma cells.
A polyclonal antisera specific for human platelet thrombospondin (TSP) has been utilized to study the biosynthesis and secretion of TSP in the M21 human melanoma cell line. Pulse-chase indirect immunoprecipitation analysis reveal that human melanoma cells rapidly synthesize and secrete this platelet alpha-granule associated glycoprotein. Topographical analysis of the melanoma cell surface by indirect immunofluorescence indicate that the TSP molecules have no obvious extracellular organization. The implications of thrombospondin synthesis in the metastatic process of melanoma are discussed.
Apelgren
L D
LD
Department of Connective Tissue and Monoclonal Antibody Research, Lilly Research Laboratories, Lilly Corporate Center, Indianapolis, IN 46285.
Bumol
T F
TF
D005455:Fluorescent Antibody Technique; D006801:Humans; D007700:Kinetics; D008546:Melanoma, Experimental / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000378:metabolism; D011233:Precipitin Tests; D019699:Thrombospondins; D014407:Tumor Cells, Cultured
D008562:Membrane Glycoproteins; D019699:Thrombospondins