Pancreastatin inhibits insulin release from Rin m 5F cells: reversion by pertussis toxin.
Pancreastatin inhibited carbachol- but not forskolin- or GIP-stimulated insulin release from Rin m 5F cells. The inhibition induced by pancreastatin was dose-dependent, with an ED50 value of 4 nM, and reached a maximum (50% of inhibition) at 10(-7) M peptide. Pretreatment of cells with pertussis toxin abolished the inhibitory effect of pancreastatin on carbachol-induced insulin release. We suggest that pancreastatin exerts a direct inhibitory control on insulin release through a pertussis toxin-sensitive cAMP-independent pathway.
Lorinet
A M
AM
INSERM U178, Unité de Recherches sur la Différenciation et al Neuroendocrinologie de Cellules Digestives, Bâtiment INSERM, Villejuif, France.
Tatemoto
K
K
Laburthe
M
M
Amiranoff
B
B
D002217:Carbachol / Q000494:pharmacology; D002478:Cells, Cultured; D053379:Chromogranin A; D007202:Indicators and Reagents; D007328:Insulin / Q000378:metabolism*; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism; D010187:Pancreatic Hormones / Q000494:pharmacology*; D037342:Pertussis Toxin; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
D053379:Chromogranin A; D007202:Indicators and Reagents; D007328:Insulin; D010187:Pancreatic Hormones; D010566:Virulence Factors, Bordetella; C050836:pancreastatin; D002217:Carbachol; D037342:Pertussis Toxin