The choice of adjuvants in Mycoplasma vaccines.
The use of adjuvants in vaccine production is an important aspect of potent vaccines. This investigation was concerned with finding the most efficient adjuvants for use in Mycoplasma vaccines produced in Nigeria. Four different vaccines were produced from the Gladysdale strain of Mycoplasma mycoides subspecies mycoides. They differed depending on the type of adjuvants used. Each vaccine was used to vaccinate eight cattle using a dose of 1 ml. Two other groups of eight cattle were used as controls. One of the two groups received 1 ml dose of inactivated Gladysdale vaccine without adjuvant while the second group received 1 ml dose of saline. The number of cattle that had the peak complement fixing (CF) antibody titres of 1/80 in each group of cattle was four for vaccine containing aluminium hydroxide gel, eight for vaccine containing liquid paraffin, one for vaccine containing sodium alginate and one for vaccine without adjuvant. Seven cattle from the group vaccinated with vaccine containing Freund's incomplete adjuvant had peak CF antibody titres of 1/80 or higher. The two groups vaccinated with vaccine containing liquid paraffin and Freund's incomplete adjuvant survived challenge at 6 months post vaccination. Freund's incomplete adjuvant and liquid paraffin containing 10% Arlacel A are the most efficient adjuvants.
Garba
S A
SA
Federal University of Technology, Minna, Nigeria.
Terry
R J
RJ
Adegboye
D S
DS
Lamorde
A G
AG
Abalaka
J A
JA
D000276:Adjuvants, Immunologic; D000464:Alginates / Q000276:immunology; D000536:Aluminum Hydroxide / Q000276:immunology; D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology*; D003168:Complement Fixation Tests; D005620:Freund's Adjuvant; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D009175:Mycoplasma Infections / Q000662:veterinary*; D009176:Mycoplasma mycoides / Q000276:immunology*; D010232:Paraffin / Q000276:immunology; D011002:Pleuropneumonia, Contagious / Q000276:immunology*; D015164:Vaccines, Inactivated / Q000276:immunology
D000276:Adjuvants, Immunologic; D000464:Alginates; D001428:Bacterial Vaccines; D006603:Hexuronic Acids; D015164:Vaccines, Inactivated; D000536:Aluminum Hydroxide; D010232:Paraffin; D020723:Glucuronic Acid; D005620:Freund's Adjuvant