Proliferative effect of interleukin-3 on normal and leukemic human B cell precursors.
We have examined the influence of recombinant, human IL-3 on normal and leukemic B cell precursors. CD10/CALLA+ leukemic B cell precursors, the pre-B cell line BLIN-1, and surface IgM-B cell precursors isolated from fetal bone marrow all responded to IL-3. This IL-3 response was dose-dependent and could be abrogated by a rabbit anti-IL-3 antiserum. Binding studies using radiolabeled IL-3 revealed the presence of approximately 200 high affinity IL-3 receptors on the BLIN-1 cell line, with a KD of 150pM. We conclude that normal and leukemic human B cell precursors express functional IL-3 receptors, extending the biologic range of this hematopoietin to the lymphoid lineage.
Wörmann
B
B
Department of Laboratory Medicine and Pathology, University of Minnesota School of Medicine, Minneapolis.
Gesner
T G
TG
Mufson
R A
RA
LeBien
T W
TW
D001402:B-Lymphocytes / Q000166:cytology / Q000378:metabolism / Q000473:pathology*; D002051:Burkitt Lymphoma / Q000378:metabolism / Q000473:pathology*; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007377:Interleukin-3 / Q000008:administration & dosage / Q000494:pharmacology*; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology*; D011485:Protein Binding; D011971:Receptors, Immunologic / Q000378:metabolism; D016185:Receptors, Interleukin-3; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000473:pathology
D007377:Interleukin-3; D011971:Receptors, Immunologic; D016185:Receptors, Interleukin-3; D011994:Recombinant Proteins