Characteristics of galactose transport in Saccharomyces cerevisiae cells and reconstituted lipid vesicles.
Growth on galactose induces two transport processes, a high-affinity and a low-affinity process. The most important results of a comparison of the two processes were that (i) both depended on GAL2 expression, (ii) only the high-affinity process required galactokinase, (iii) both were down-regulated by catabolite inactivation, (iv) neither was significantly inhibited by carbonyl cyanide-p-trifluoromethoxy-phenyl-hydrazone, (v) neither was differentially inhibited by silver nitrate or mercuric chloride, and (vi) transport activity with a Km closer to that of the low-affinity process of whole cells was reconstituted in fused phospholipid membrane vesicles.
Ramos
J
J
Department of Biochemistry, State University of New York, Stony Brook 11794.
Szkutnicka
K
K
Cirillo
V P
VP
D001692:Biological Transport / Q000187:drug effects; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology; D002412:Cations; D002474:Cell-Free System; D005686:Galactokinase / Q000502:physiology; D005690:Galactose / Q000378:metabolism*; D005800:Genes, Fungal; D005947:Glucose / Q000502:physiology; D066298:In Vitro Techniques; D007700:Kinetics; D008628:Mercury / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D012834:Silver / Q000494:pharmacology
D002412:Cations; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; D012834:Silver; D005686:Galactokinase; D008628:Mercury; D005947:Glucose; D005690:Galactose