Esterification of chiral secondary alcohols with fatty acid in organic solvents by polyethylene glycol-modified lipase.
Lipase from Pseudomonas fragi 22.39B was modified with polyethylene glycol. The modified lipase (PEG-lipase) was soluble and active in organic solvents such as benzene and 1,1,1-trichloroethane. PEG-lipase catalyzed esterification of chiral secondary alcohols with fatty acids in benzene and exhibited preference for R isomers over S isomers. Km and Vmax values for each isomer of various alcohols were obtained by kinetic study of the esterification in benzene. PEG-lipase-catalyzed esterification leads to optical resolution of a racemic alcohol.
Kikkawa
S
S
Department of Life Science, Faculty of Science, Tokyo Institute of Technology, Sagami Chemical Research Center, Japan.
Takahashi
K
K
Katada
T
T
Inada
Y
Y
D000438:Alcohols / Q000378:metabolism*; D004951:Esterification; D005227:Fatty Acids / Q000378:metabolism*; D066298:In Vitro Techniques; D008049:Lipase / Q000378:metabolism*; D000442:Octanols / Q000493:pharmacokinetics; D011092:Polyethylene Glycols / Q000494:pharmacology; D011549:Pseudomonas
D000438:Alcohols; D005227:Fatty Acids; D000442:Octanols; D011092:Polyethylene Glycols; D008049:Lipase