title: Kinetics of human hemopoietic cells after in vivo administration of granulocyte-macrophage colony-stimulating factor.
abstract: The kinetic changes induced by granulocyte-macrophage colony-stimulating factor (GM-CSF) on hemopoietic cells were assessed in physiological conditions by administering GM-CSF (8 micrograms/kg per d) for 3 d to nine patients with solid tumors and normal bone marrow (BM), before chemotherapy. GM-CSF increased the number of circulating granulocytes and monocytes; platelets, erythrocytes, lymphocyte number, and subsets were unmodified. GM-CSF increased the percentage of BM S phase BFU-E (from 32 +/- 7 to 79 +/- 16%), day 14 colony-forming unit granulocyte-macrophage (CFU-GM) (from 43 +/- 20 to 82 +/- 11%) and day 7 CFU-GM (from 41 +/- 14 to 56 +/- 20%). The percentage of BM myeloblasts, promyelocytes, and myelocytes in S phase increased from 26 +/- 14 to 41 +/- 6%, and that of erythroblasts increased from 25 +/- 12 to 30 +/- 12%. This suggests that GM-CSF activates both erythroid and granulomonopoietic progenitors but that, among the morphologically recognizable BM precursors, only the granulomonopoietic lineage is a direct target of the molecule. GM-CSF increased the birth rate of cycling cells from 1.3 to 3.4 cells %/h and decreased the duration of the S phase from 14.3 to 9.1 h and the cell cycle time from 86 to 26 h. After treatment discontinuation, the number of circulating granulocytes and monocytes rapidly fell. The proportion of S phase BM cells dropped to values lower than pretreatment levels, suggesting a period of relative refractoriness to cell cycle-active antineoplastic agents.
authors.affiliation: Dipartimento di Scienze Biomediche ed Oncologia Umana dell'Universitá, Torino, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Aglietta
authors.forename: W
authors.initials: W
authors.lastname: Piacibello
authors.forename: F
authors.initials: F
authors.lastname: Sanavio
authors.forename: A
authors.initials: A
authors.lastname: Stacchini
authors.forename: F
authors.initials: F
authors.lastname: Aprá
authors.forename: M
authors.initials: M
authors.lastname: Schena
authors.forename: C
authors.initials: C
authors.lastname: Mossetti
authors.forename: F
authors.initials: F
authors.lastname: Carnino
authors.forename: F
authors.initials: F
authors.lastname: Caligaris-Cappio
authors.forename: F
authors.initials: F
authors.lastname: Gavosto
mesh_terms: D001853:Bone Marrow / Q000187:drug effects; D002455:Cell Division; D003115:Colony-Stimulating Factors / Q000494:pharmacology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D006801:Humans; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology
chemical_list: D003115:Colony-Stimulating Factors; D006133:Growth Substances; D016178:Granulocyte-Macrophage Colony-Stimulating Factor