title: Cell fractionation studies indicate that dystrophin is a protein of surface membranes of skeletal muscle.
abstract: We studied the subcellular localization of dystrophin in rabbit skeletal muscle. In Western-blot analysis of membrane preparations, dystrophin was associated with the sarcolemmal fraction, as indicated by cholesterol content and co-purification with ouabain-binding activity and beta-adrenergic receptor. Dystrophin was also found with junctional T-tubules, but not with 'free' T-tubules, longitudinal portions or terminal cisternae of the sarcoplasmic reticulum. Dystrophin was not solubilized by high salt solutions, but it was solubilized by low concentrations of detergents (Triton X-100 and deoxycholate), suggesting that it is a peripheral membrane protein.
authors.affiliation: Centro di Studio per la Biologia e la Fisiopatologia Muscolare, Universita' di Padova, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Salviati
authors.forename: R
authors.initials: R
authors.lastname: Betto
authors.forename: S
authors.initials: S
authors.lastname: Ceoldo
authors.forename: E
authors.initials: E
authors.lastname: Biasia
authors.forename: E
authors.initials: E
authors.lastname: Bonilla
authors.forename: A F
authors.initials: AF
authors.lastname: Miranda
authors.forename: S
authors.initials: S
authors.lastname: Dimauro
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002784:Cholesterol / Q000032:analysis; D016189:Dystrophin; D008565:Membrane Proteins / Q000032:analysis*; D009124:Muscle Proteins / Q000032:analysis*; D011817:Rabbits; D012508:Sarcolemma / Q000032:analysis*; D012519:Sarcoplasmic Reticulum / Q000032:analysis; D012995:Solubility
chemical_list: D016189:Dystrophin; D008565:Membrane Proteins; D009124:Muscle Proteins; D002784:Cholesterol