Multilabeled 13C substrates as probes in in vivo 13C and 1H NMR spectroscopy.
The potential use of 13C multilabeled substrates has been studied in biological applications using in vivo and in vitro proton and 13C NMR spectroscopy. In 13C NMR spectroscopy, multilabeled compounds allow the simultaneous observation of several nuclei or increase distinctly the signal to noise ratio due to a higher degree of enrichment. Contiguous labeling of substrates leads to homonuclear 13C-13C spin couplings and provides a simple means to distinguish between endogenous stores of metabolites and metabolites derived from added substrates.
Künnecke
B
B
Biocenter of the University of Basel, Switzerland.
Cerdan
S
S
D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism; D008099:Liver / Q000033:anatomy & histology / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D051381:Rats; D011919:Rats, Inbred Strains