Preparation of Escherichia coli elongation factor Tu-guanosine 5'-triphosphate analogs.
A simple procedure for the bulk preparation of 20 mg of Escherichia coli elongation factor (EF)-Tu-GTP analogs is described. The protocol is based upon the preparation and stabilization of nucleotide-free EF-Tu using an EF-Ts affinity chromatographic resin. The procedure is a general one for the preparation of any GTP analog of EF-Tu.
Delaria
K
K
Department of Biochemistry, University of California, Riverside 92521.
Jurnak
F
F
D001667:Binding, Competitive; D002846:Chromatography, Affinity; D004926:Escherichia coli; D006150:Guanine Nucleotides / Q000302:isolation & purification; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D010444:Peptide Elongation Factor Tu / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D010445:Peptide Elongation Factors; D012116:Resins, Plant; D012685:Sepharose
D006150:Guanine Nucleotides; D010445:Peptide Elongation Factors; D012116:Resins, Plant; C023512:elongation factor Ts; D006160:Guanosine Triphosphate; D012685:Sepharose; D010444:Peptide Elongation Factor Tu