title: Assessing clamp-related vascular injuries by measurement of associated vascular dysfunction.
abstract: The development of vascular clamps requires a reliable method to quantitate clamp-related vascular injuries. The degree of vessel damage usually is estimated subjectively from photomicrographs made with scanning electron microscopy. In order to test whether the use of vascular rings to assess residual vascular function may be a better method, rabbit thoracic aortas were occluded by five types of clamps: a Fogarty softjaw bulldog, a Fogarty Hydragrip, a 6-inch Satinsky clamp, an Edslab bulldog, and a silicone vessel band. Each area of clamp injury was sectioned into a vascular ring and suspended in a tissue bath. Residual vascular function was determined by contraction in response to phenylephrine and by relaxation in response to methacholine chloride and sodium nitroprusside. Morphologic studies with use of Evans blue dye and scanning electron microscopy complemented the vascular-function studies. The Fogarty bulldog clamp was the best at preserving vascular contraction and relaxation; the more crushing Satinsky clamp was the worst. The testing of vascular rings for residual function appears to be a useful technique for objectively quantitating vascular clamp-related vascular damage.
authors.affiliation: Department of Surgery, University of Virginia Medical Center, Charlottesville 22908.
authors.forename: G W
authors.initials: GW
authors.lastname: Barone
authors.forename: J M
authors.initials: JM
authors.lastname: Conerly
authors.forename: P C
authors.initials: PC
authors.lastname: Farley
authors.forename: T L
authors.initials: TL
authors.lastname: Flanagan
authors.forename: I L
authors.initials: IL
authors.lastname: Kron
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000187:drug effects / Q000293:injuries* / Q000503:physiopathology; D003250:Constriction / Q000009:adverse effects* / Q000295:instrumentation; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D016210:Methacholine Chloride; D008688:Methacholine Compounds / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D009599:Nitroprusside / Q000494:pharmacology; D010656:Phenylephrine / Q000494:pharmacology; D011817:Rabbits; D014661:Vasoconstriction / Q000187:drug effects; D014664:Vasodilation
chemical_list: D008688:Methacholine Compounds; D016210:Methacholine Chloride; D009599:Nitroprusside; D010656:Phenylephrine