Measurement of cyclosporine concentrations in whole blood: HPLC and radioimmunoassay with a specific monoclonal antibody and 3H- or 125I-labeled ligand compared.
We compared cyclosporine concentrations in whole blood as measured by HPLC and by RIA with a monoclonal antibody specific for cyclosporine with 3H- or 125I-labeled cyclosporine ligand. The 3H-RIA kit slightly underestimated cyclosporine concentrations (greater than 600 micrograms/L) in comparison with HPLC. Over a wide range of concentrations, cyclosporine measured with the 125I-RIA kit correlated well with HPLC (slope = 0.99, n = 301, r = 0.98), observed for samples from recipients of kidney, heart, or liver allografts (respective slopes: 1.01, 0.93, and 1.00). The 125I-RIA standard curve was linear to 1000 micrograms of cyclosporine per liter. Inter- and intra-assay CVs for 125I-RIA measurements of cyclosporine were less than or equal to 7%. Evidently, the 125I-RIA kit involving a monoclonal antibody specific for cyclosporine is equivalent to the HPLC assay and can replace it for therapeutic drug monitoring of cyclosporine therapy.
Wolf
B A
BA
Department of Pathology, Washington University School of Medicine, St. Louis, MO 63110.
Daft
M C
MC
Koenig
J W
JW
Flye
M W
MW
Turk
J W
JW
Scott
M G
MG
D000911:Antibodies, Monoclonal; D002851:Chromatography, High Pressure Liquid; D003524:Cyclosporins / Q000097:blood* / Q000627:therapeutic use; D016027:Heart Transplantation; D006801:Humans; D007457:Iodine Radioisotopes; D016030:Kidney Transplantation; D016031:Liver Transplantation; D011863:Radioimmunoassay; D012044:Regression Analysis; D014316:Tritium
D000911:Antibodies, Monoclonal; D003524:Cyclosporins; D007457:Iodine Radioisotopes; D014316:Tritium