N-RAS mutations in T-cell acute lymphocytic leukaemia: analysis by direct sequencing detects a novel mutation.
A novel mutation of the N-RAS gene of T-ALL blast cells was detected by a direct sequencing of in vitro amplified exon-1 of the N-RAS gene. Threonine (ACA) was substituted for alanine (GCA) at codon 11. This mutation would have been overlooked by conventional probe hybridization techniques. A search for other mutations in N-RAS exon-1 in T-ALL revealed a codon 13 mutation substituting aspartic acid (GAT) for glycine (GGT) in one of 18 patients. No mutations at codon 12 were detected.
Bar-Eli
M
M
Division of Hematology/Oncology, University of California, Los Angeles 90024-1678.
Ahuja
H
H
Foti
A
A
Cline
M J
MJ
D001483:Base Sequence; D003062:Codon; D005091:Exons; D011905:Genes, ras; D006801:Humans; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000235:genetics*; D009154:Mutation
D003062:Codon