title: Glass-metal keratoprosthesis: light and electron microscopical evaluation of experimental surgery on rabbit eyes.
abstract: A keratoprosthesis (KP) is the last and only surgical resort to regain some visual acuity in eyes with severely damaged corneae. Corneal blindness represents an important percentage of the blind in the economically poor countries. Commercially available KP's, e.g. those made of PMMA, which are difficult to sterilize and vulnerable to surface damage, are too expensive in these countries. To overcome these disadvantages, we developed a new KP, made of a glass core melted into a platinum cylinder with flange. They were implanted unilaterally in eyes of ten Hollander rabbits intralamellarly. They were fixated by two stainless steel traction threads passed around the whole eyeball. We investigated this type of KP in the rabbit cornea, its acceptance by stroma, epi- and endothelium, and its hydro-mechanical dynamics in situ. No signs of infection or extrusion were observed. No epithelial downgrowth, nor adverse tissue reaction could be detected. LM and SEM showed endothelialization of the newly formed stroma around the central column of the KP. We conclude that this type of KP (although optically still to be optimized) has been accepted by the rabbit cornea and a clinical trial on cornea-blind patients is justified.
authors.affiliation: University of Groningen, Department of Histology and Cell Biology, The Netherlands.
authors.forename: P L
authors.initials: PL
authors.lastname: Cuperus
authors.forename: W L
authors.initials: WL
authors.lastname: Jongebloed
authors.forename: P
authors.initials: P
authors.lastname: van Andel
authors.forename: J G
authors.initials: JG
authors.lastname: Worst
mesh_terms: D000818:Animals; D003315:Cornea / Q000000981:diagnostic imaging / Q000648:ultrastructure; D003319:Corneal Stroma / Q000648:ultrastructure; D016039:Corneal Transplantation; D004728:Endothelium, Corneal / Q000648:ultrastructure; D004847:Epithelial Cells; D005898:Glass; D010984:Platinum; D011182:Postoperative Care; D019736:Prostheses and Implants; D011474:Prosthesis Design; D011817:Rabbits; D011859:Radiography; D013997:Time Factors
chemical_list: D010984:Platinum