Co-localization of a glucose transporter and the insulin receptor in microsomes of insulin-treated rat adipocytes.
Microsomal vesicles prepared from rat adipocytes were immuno-adsorbed to formaldehyde-fixed Staphylococcus aureus cells (Pansorbin) coated with anti-human-erythrocyte-glucose-transporter IgG. More than 75% of the glucose transporter detected was precipitated. The glucose transporter was about 10-fold enriched by the adsorption procedure. On insulin treatment, the insulin receptor in plasma membranes was internalized and the receptor in the microsome fraction increased 5-fold. Thirty-five % of the insulin receptor in the microsome fraction was recovered with the glucose-transporter-containing vesicles. These observations indicate that on insulin treatment a considerable portion of the microsome vesicles containing the insulin receptor fuses or becomes tightly associated with ones containing the glucose transporter.
Ezaki
O
O
Division of Nutrition and Disease, National Institute of Nutrition, Tokyo, Japan.
Bono
N
N
Itakura
H
H
Kasahara
M
M
D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D015151:Immunoblotting; D007163:Immunosorbent Techniques; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology*; D008297:Male; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism*; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D051381:Rats; D011972:Receptor, Insulin / Q000378:metabolism*
D007328:Insulin; D009004:Monosaccharide Transport Proteins; D011972:Receptor, Insulin