title: Genetic characterization of frameshift suppressors with new decoding properties.
abstract: Suppressor mutants that cause ribosomes to shift reading frame at specific and new sequences are described. Suppressors for trpE91, the only known suppressible -1 frameshift mutant, have been isolated in Escherichia coli and in Salmonella typhimurium. E. coli hopR acts on trpE91 within the 9-base-pair sequence GGA GUG UGA, is dominant, and is located at min 52 on the chromosome. Its Salmonella homolog maps at an equivalent position and arises as a rarer class in that organism as compared with E. coli. The Salmonella suppressor, hopE, believed to be in a duplicate copy of the same gene, maps at min 17. The +1 suppressor, sufT, acts at the nonmonotonous sequence CCGU, is dominant, and maps at min 59 on the Salmonella chromosome.
authors.affiliation: Department of Genetics, Trinity College, Dublin, Ireland.
authors.forename: D
authors.initials: D
authors.lastname: Hughes
authors.forename: S
authors.initials: S
authors.lastname: Thompson
authors.forename: M
authors.initials: M
authors.lastname: O'Connor
authors.forename: T
authors.initials: T
authors.lastname: Tuohy
authors.forename: B P
authors.initials: BP
authors.lastname: Nichols
authors.forename: J F
authors.initials: JF
authors.lastname: Atkins
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005838:Genotype; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids; D012486:Salmonella typhimurium / Q000235:genetics*; D013489:Suppression, Genetic; D014161:Transduction, Genetic