[A diagnostic kit for rapid microenzyme linked immunosorbent assay of paragonimiasis].
With a diagnostic kit for paragonimiasis we recently developed, 112 active paragonimiasis cases were examined and 111 (99.1%) of them showed positive reaction. Sera of 100 healthy persons all showed negative reaction, while 2.3% (3/130) of the patients with other parasitic diseases showed a week cross reaction. Sera of 40 cases with non-parasitic pulmonary diseases also showed negative reaction. The reciprocal titre of cases of paragonimiasis in terms of geometrical mean (GMRT) versus that of normal person was 1396 to 21 (P less than 0.0001). The reproduction and stability of this kit was satisfactory and no significant changes were noted when being kept in room temperature for 12 weeks or being treated with heat (37 degrees C 30', 56 degrees C 30', 64 degrees C 30', 100 degrees C 1', 2', 3', in water bath). When the antigen was treated in 100 degrees C water bath for 30', the OD value obtained with positive control serum showed a slight decrease, but OD value of the positive specimen was still 2.1 times higher than that of negative specimen. There was no significant difference whether the antigen was lyophilized or not. When the antigen was purified by sephadex G 100 gel column, the G100-1 portion showed higher antigenic activity and specificity, but it was not as sensitive as the crude antigen.
Zhang
Y Q
YQ
Wen
Y
Y
Gui
S H
SH
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D010237:Paragonimiasis / Q000175:diagnosis*; D010238:Paragonimus / Q000276:immunology; D011933:Reagent Kits, Diagnostic
D000947:Antigens, Helminth; D011933:Reagent Kits, Diagnostic