title: Combination of the APAAP and the IGSS techniques for double-labeling with two different monoclonal antibodies.
abstract: Cryostat sections were incubated with two different monoclonal antibodies directed either against two different cells or against the same cell. For visualization of the first antibody, the alkaline phosphatase-antialkaline phosphatase (APAAP) technique was used, which rendered a bright red reaction product. For visualization of the second monoclonal antibody, the immunogold-silver-staining (IGSS) technique was used, which resulted in a black reaction product. The combination of an enzyme technique with a technique involving metals (gold and silver) seemed ideal since the reaction product of either technique could be detected easily.
authors.affiliation: Department of Dermatology, Eberhard-Karls University, Tübingen, F.R.G.
authors.forename: G
authors.initials: G
authors.lastname: Schaumburg-Lever
authors.forename: M
authors.initials: M
authors.lastname: Tronnier
mesh_terms: D000469:Alkaline Phosphatase; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000473:pathology; D006046:Gold; D006801:Humans; D007124:Immunoenzyme Techniques; D007801:Langerhans Cells / Q000473:pathology; D012394:Rosaniline Dyes; D012834:Silver; D013601:T-Lymphocytes / Q000473:pathology
chemical_list: D000911:Antibodies, Monoclonal; D012394:Rosaniline Dyes; D012834:Silver; D006046:Gold; D000469:Alkaline Phosphatase