High frequency of Ki-ras codon 12 mutations in pancreatic adenocarcinomas.
The frequency of Ki-ras gene mutations was studied in 100 paraffin-embedded sections obtained from 63 pancreatic adenocarcinomas by in vitro amplification of target sequences via polymerase chain reaction (PCR) and selective oligonucleotide hybridization. Forty-seven (75%) of the tumors contained a Ki-ras mutation at codon 12. No predominant amino acid substitution or nucleotide transition at this codon was observed. Two carcinomas exhibited 2 distinct Ki-ras mutations. No particular correlation could be established between the incidence of Ki-ras mutation and clinical parameters (sex, age, survival), tumor grade or tumor stage.
Grünewald
K
K
Department of Pediatrics II, University of Ulm, FRG.
Lyons
J
J
Fröhlich
A
A
Feichtinger
H
H
Weger
R A
RA
Schwab
G
G
Janssen
J W
JW
Bartram
C R
CR
D000230:Adenocarcinoma / Q000235:genetics*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003062:Codon / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D011905:Genes, ras; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D021141:Nucleic Acid Amplification Techniques; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D010190:Pancreatic Neoplasms / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*
D003062:Codon; D004273:DNA, Neoplasm; D015345:Oligonucleotide Probes; D012333:RNA, Messenger