title: Phenytoin and phenobarbital concentrations in serum: a comparison of Ames Seralyzer with GLC, TDX, and EMIT.
abstract: A recently developed system for measuring antiepileptic drug concentrations was evaluated for phenytoin and phenobarbital. The apoenzyme reactive immunoassay system was compared with gas-liquid chromatography, EMIT, and TDX systems. Comparisons were performed with control specimens and with sera obtained from patients at three facilities. Precision for all methods was similar, with within-run and between-run coefficients of variation generally 5%. The accuracy of all methods was acceptable, but bias was present in some. However, measurements obtained by a nontechnical person (physician) in a clinical setting were sometimes inaccurate.
authors.affiliation: Ramsey Clinic, St. Paul, Minnesota.
authors.forename: I E
authors.initials: IE
authors.lastname: Leppik
authors.forename: K S
authors.initials: KS
authors.lastname: Oles
authors.forename: M L
authors.initials: ML
authors.lastname: Sheehan
authors.forename: J K
authors.initials: JK
authors.lastname: Penry
authors.forename: D R
authors.initials: DR
authors.lastname: Parker
authors.forename: T K
authors.initials: TK
authors.lastname: Gallagher
authors.forename: G P
authors.initials: GP
authors.lastname: Caron
authors.forename: W
authors.initials: W
authors.lastname: Rosenfeld
authors.forename: J J
authors.initials: JJ
authors.lastname: Salmon
mesh_terms: D002849:Chromatography, Gas; D006801:Humans; D007124:Immunoenzyme Techniques; D015337:Multicenter Studies as Topic; D010634:Phenobarbital / Q000097:blood*; D010672:Phenytoin / Q000097:blood*
chemical_list: D010672:Phenytoin; D010634:Phenobarbital