Effect of noradrenaline on triacylglycerol synthesis in rat brown adipocytes.
Incubation of rat brown adipocytes with noradrenaline in the presence of insulin and palmitate caused a decrease in the rate of triacylglycerol synthesis as measured by [U-14C]glucose incorporation into acylglycerol glycerol. Concomitantly, the ratio of [1-14C]palmitate oxidized to CO2 to that esterified was increased. This alteration in the rate of triacylglycerol synthesis by noradrenaline was not observed when fatty acid oxidation was inhibited by etomoxir. Noradrenaline did not cause any acute inactivation of enzymes of the triacylglycerol-synthesis pathway. It is suggested that the decrease in triacylglycerol synthesis seen with noradrenaline is secondary to activation of fatty acid oxidation.
Baht
H S
HS
Department of Biochemistry, University College London, U.K.
Saggerson
E D
ED
D002001:Adipose Tissue, Brown / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D004852:Epoxy Compounds / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology*; D010168:Palmitates / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D014280:Triglycerides / Q000096:biosynthesis*
D004852:Epoxy Compounds; D007328:Insulin; D010168:Palmitates; D014280:Triglycerides; C054207:etomoxir; D009638:Norepinephrine