title: Proto-oncogene expression during early myeloid differentiation of normal peripheral blood progenitor cells.
abstract: Peripheral blood mononuclear cells cultured in liquid medium with fetal calf serum undergo differentiation to myeloblasts (7 days) and then mature granulocytes (14-21 days). This culture system was used to study proto-oncogene expression during pre-myeloblast myeloid differentiation. c-myc mRNA was present in the peripheral blood mononuclear cells placed into culture, fell during the first 2-4 days of culture and then rose between days 2-4 and day 7 of culture, prior to and coincident with the appearance of myeloblasts. Histone H3 mRNA was absent or present at very low levels at initiation of cultures, and then rose throughout the first 7 days of culture. c-fms mRNA was absent at initiation of cultures, and appeared on days 2-5 of culture, prior to the appearance of myeloblasts. c-fos mRNA was not detected during differentiation of peripheral blood mononuclear cells to myeloblasts. Elucidation of patterns of proto-oncogene expression during normal myeloid differentiation is a prerequisite for interpretation of proto-oncogene expression in myeloid leukemia cells.
authors.affiliation: Department of Hematologic Oncology, Roswell Park Memorial Institute, Buffalo, NY 14263.
authors.forename: M R
authors.initials: MR
authors.lastname: Baer
authors.forename: X Z
authors.initials: XZ
authors.lastname: Gao
authors.forename: H
authors.initials: H
authors.lastname: Sato
authors.forename: P
authors.initials: P
authors.lastname: Singh
authors.forename: H D
authors.initials: HD
authors.lastname: Preisler
mesh_terms: D015152:Blotting, Northern; D002454:Cell Differentiation; D002478:Cells, Cultured; D006098:Granulocytes / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006657:Histones / Q000378:metabolism; D006801:Humans; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D006657:Histones; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger