Expression of human interleukin-3 (multi-CSF) is restricted to human lymphocytes and T-cell tumor lines.
While the cellular sources for granulocyte-macrophage colony-stimulating factor (GM-CSF) are known to be widely distributed among several cell types, interleukin-3 (IL-3) gene expression has been demonstrated in only certain T-cell clones and in blood mononuclear cells stimulated with phytohemagglutinin (PHA) and phorbol-myristate-acetate (PMA). To determine which blood cells were responsible for this expression, we fractionated PHA/PMA-stimulated mononuclear cells and identified T lymphocytes as the source of IL-3 mRNA. Low-level IL-3 expression was detected as well in several stimulated human T-cell lines. Hematopoietic stromal cells such as fibroblasts and endothelial cells could not be induced to express IL-3 mRNA. The kinetics of IL-3 mRNA induction in mononuclear cells and lymphocytes stimulated with PHA/PMA or anti-CD3 monoclonal antibody (MoAb) and interleukin-1 (IL-1) were similar to those observed for GM-CSF expression.
Niemeyer
C M
CM
Division of Hematology and Pediatric Oncology, Children's Hospital, Boston, Massachusetts.
Sieff
C A
CA
Mathey-Prevot
B
B
Wimperis
J Z
JZ
Bierer
B E
BE
Clark
S C
SC
Nathan
D G
DG
D003115:Colony-Stimulating Factors / Q000032:analysis* / Q000235:genetics; D005786:Gene Expression Regulation; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000032:analysis* / Q000235:genetics; D006801:Humans; D007377:Interleukin-3 / Q000032:analysis* / Q000235:genetics; D008214:Lymphocytes / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D013601:T-Lymphocytes / Q000032:analysis*; D014407:Tumor Cells, Cultured
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D007377:Interleukin-3; D012333:RNA, Messenger; D016178:Granulocyte-Macrophage Colony-Stimulating Factor