Proteolytic enzymes in excretory-secretory products from adult Nematospiroides dubius.
Excretory-secretory products (ES), collected from in vitro cultures of adult Nematospiroides dubius, were examined for proteolytic enzyme activity. ES enzymes had a pH optimum of 8.0 and their activity was sensitive to serine-proteinase inhibitors. Three SDS-resistant proteases were identified in ES at molecular weight (mol. wt) 200,000, 105,000 and 48,000 by incorporating substrates into the matrices of sodium dodecyl sulfate-polyacrylamide electrophoresis (SDS-PAGE) gels.
Monroy
F G
FG
Cayzer
C J
CJ
Adams
J H
JH
Dobson
C
C
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D006369:Heligmosomatoidea / Q000201:enzymology*; D009351:Nematospiroides dubius / Q000201:enzymology*; D010447:Peptide Hydrolases / Q000032:analysis*; D011480:Protease Inhibitors / Q000494:pharmacology
D011480:Protease Inhibitors; D010447:Peptide Hydrolases