Identification of human spermatozoa antigens using monoclonal antibodies and the alkaline phosphatase anti-alkaline phosphatase-technique.
Both cytoplasmic and surface-membrane antigens of human spermatozoa were detected by means of monoclonal antibodies (MoAbs) and of the alkaline phosphatase anti-alkaline phosphatase- (APAAP-) technique. Several advantages of this technique for the identification of sperm could be demonstrated. The labeling of cytocentrifuge preparations from 16 ejaculates proved the presence of glycosphingolipids, nuclear and mitochondrial antigens of spermatozoa. However, there were no HLA-molecules and other leukocyte antigens on sperm cells.
Schöbel
W A
WA
Dermatologie II, Hautklinik der Universität, Tübingen.
Schieferstein
G
G
Knapp
R
R
Uchanska-Ziegler
B
B
Ziegler
A
A
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000276:immunology*; D006028:Glycosphingolipids / Q000276:immunology; D006680:HLA Antigens / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D013094:Spermatozoa / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D006028:Glycosphingolipids; D006680:HLA Antigens