[Effect of lobenzarit disodium (CCA) on B cell differentiation to antibody secreting cells].
We investigated the effect of the immunomodulator CCA on B cells, using the system of Staphylococcus aureus Cowan 1-stimulated B cells developing into antibody secreting cells in the presence of T cell factors. Results were expressed as the number of plaque-forming cells (PFC), as measured by reverse hemolytic plaque assay. Serially diluted CCA was added to the culture system to evaluate its effect (500-0.005 micrograms/ml). Different results were obtained by adding CCA at a high dose or a low dose. High-dose CCA showed an inhibitory effect on PFC (p less than 0.01). On the other hand, low-dose CCA showed an inhibitory effect on high PFC responses of B cells and a stimulatory effect on low responses. Our data suggest that CCA has a direct effect on B cells and that low-dose CCA has immunomodulatory action on normal variations in immune responses.
Imai
F
F
Suzuki
T
T
Ishibashi
T
T
Katagiri
T
T
Hara
K
K
D000921:Antibody-Producing Cells / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006462:Hemolytic Plaque Technique; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects; D062367:ortho-Aminobenzoates / Q000494:pharmacology*
D062367:ortho-Aminobenzoates; C015735:lobenzarit