Molecular cloning and sequencing of a cDNA encoding N alpha-acetyltransferase from Saccharomyces cerevisiae.
Acetylation is the most frequently occurring chemical modification of the alpha-NH2 group of eukaryotic proteins and is catalyzed by an N alpha-acetyltransferase. Recently, a eukaryotic N alpha-acetyltransferase was purified to homogeneity from Saccharomyces cerevisiae, and its substrate specificity was partially characterized (Lee, F.-J. S., Lin L.-W., and Smith, J. A. (1988) J. Biol. Chem. 263, 14948-14955). This article describes the cloning from a yeast lambda gt11 cDNA library and sequencing of a full length cDNA encoding yeast N alpha-acetyltransferase. DNA blot hybridizations of genomic and chromosomal DNA reveal that the gene (so-called AAA1, amino-terminal, alpha-amino, acetyltransferase) is present as a single copy located on chromosome IV. The use of this cDNA will allow the molecular details of the role of N alpha-acetylation in the sorting and degradation of eukaryotic proteins to be determined.
Lee
F J
FJ
Department of Molecular Biology, Massachusetts General Hospital, Boston.
Lin
L W
LW
Smith
J A
JA
D000123:Acetyltransferases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics*; D008969:Molecular Sequence Data; D063206:N-Terminal Acetyltransferases; D015345:Oligonucleotide Probes; D010446:Peptide Fragments / Q000032:analysis; D011487:Protein Conformation; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D014357:Trypsin
D004271:DNA, Fungal; D015345:Oligonucleotide Probes; D010446:Peptide Fragments; D000123:Acetyltransferases; D063206:N-Terminal Acetyltransferases; D014357:Trypsin