Identification and characterization of macrophage inflammatory protein 2.
In response to endotoxin, macrophages secrete a protein with a molecular mass of approximately 6000 Da and with an affinity for heparin. This protein, which we term "macrophage inflammatory protein 2," is a potent chemotactic agent for human polymorphonuclear leukocytes. In addition, subcutaneous administration of the monokine causes a localized inflammatory reaction. Partial N-terminal sequence data reveal similarity to a family of proteins, the archetype of which is platelet factor 4. Although macrophage inflammatory protein 2 is a distinct member of the platelet factor 4 family, its sequence is most closely related to that of the gro/KC gene product, which is expressed in transformed or platelet-derived growth factor-treated cells.
Wolpe
S D
SD
Laboratory of Medical Biochemistry, Rockefeller University, New York, NY 10021-6399.
Sherry
B
B
Juers
D
D
Davatelis
G
G
Yurt
R W
RW
Cerami
A
A
D000595:Amino Acid Sequence; D000818:Animals; D054426:Chemokine CXCL2; D019743:Chemokines, CXC; D002630:Chemotactic Factors / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D002634:Chemotaxis, Leukocyte; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D006861:Hydrogen Peroxide / Q000378:metabolism; D015151:Immunoblotting; D036341:Intercellular Signaling Peptides and Proteins; D016209:Interleukin-8; D008264:Macrophages / Q000032:analysis*; D051379:Mice; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000276:immunology
C499706:CXCL2 protein, human; D054426:Chemokine CXCL2; D019743:Chemokines, CXC; D002630:Chemotactic Factors; D036341:Intercellular Signaling Peptides and Proteins; D016209:Interleukin-8; D006861:Hydrogen Peroxide