Clostridium botulinum type D neurotoxin: purification and detection.
A method is reported for the purification of type D botulinum toxin using a combination of low and high pressure ion exchange chromatography. The procedure produced homogeneous toxin in its free form in 3 days, with a specific toxicity in mice of 5.4 x 10(7) LD50/mg protein. Polyclonal antibodies against the pure toxin were raised in rabbits and detected the toxin in both ELISA and western blotting. The antibodies also detected type C1 botulinum toxin using these techniques, confirming the presence of cross-reacting antigenic determinants in these two proteins.
De Jongh
K S
KS
School of Chemistry, Macquarie University, North Ryde, Australia.
Schwartzkoff
C L
CL
Howden
M E
ME
D000818:Animals; D001681:Biological Assay; D001905:Botulinum Toxins / Q000032:analysis*; D002848:Chromatography, DEAE-Cellulose; D002852:Chromatography, Ion Exchange; D003014:Clostridium botulinum / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D009498:Neurotoxins / Q000032:analysis*
D009498:Neurotoxins; D001905:Botulinum Toxins