Infection of cultured human fetal pancreatic islet cells by rubella virus.
A high incidence of insulin-dependent diabetes mellitus (IDDM) has been reported in children and young adults previously afflicted with congenital rubella syndrome (CRS). The authors have studied the effect of rubella virus infection on human pancreatic islet cells in tissue culture. These experiments were performed with the use of both monolayers and free-floating human fetal islets of Langerhans tissue. Levels of production of immunoreactive insulin by islet cells that had been infected by rubella virus were lower than those observed in control cultures, under conditions of high glucose concentration (11.1 mmol/L) in the medium. The presence of rubella viral antigens in human pancreatic beta and non-beta cells was demonstrated by double-label immunofluorescence. These results suggest that rubella virus can infect human pancreatic islet cells and that such infection may lead to significant reductions in levels of secreted insulin.
Numazaki
K
K
McGill University-Montreal Children's Hospital Research Institute, Quebec, Canada.
Goldman
H
H
Wong
I
I
Wainberg
M A
MA
D000956:Antigens, Viral / Q000032:analysis; D002478:Cells, Cultured; D003922:Diabetes Mellitus, Type 1 / Q000209:etiology; D005333:Fetus / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D007328:Insulin / Q000032:analysis*; D007515:Islets of Langerhans / Q000378:metabolism* / Q000382:microbiology; D012409:Rubella / Q000378:metabolism*; D012410:Rubella Syndrome, Congenital / Q000150:complications; D012412:Rubella virus / Q000276:immunology; D013806:Theophylline / Q000494:pharmacology
D000956:Antigens, Viral; D007328:Insulin; D013806:Theophylline; D005947:Glucose