title: Evaluation of an enzyme immunochromatography method for carbamazepine: a comparison with enzyme-multiplied immunoassay technique, fluorescence polarization immunoassay, and high-performance liquid chromatography.
abstract: A noninstrumented enzyme immunochromatography (EIC) method for monitoring carbamazepine using whole blood was compared to the enzyme-multiplied immunoassay technique (EMIT), fluorescence polarization immunoassay (FPIA), and high-performance liquid chromatography (HPLC). Samples from 74 patients were evaluated in the comparison study, yielding correlation coefficients of 0.961 (EMIT), 0.974 (FPIA), and 0.867 (HPLC). The EIC method produced within-run coefficients of variation of 4.3%, 4.9%, and 5.8% for three carbamazepine concentrations. The between-run coefficient of variation over 107 days was 4.9%. The spiked serum sample analysis yielded recovery rates ranging from 98 to 102%. Enzyme immunochromatography was found to be a useful noninstrumented method for on-site testing. The test gives quantitative patient sample results comparable to the results obtained using established laboratory methods.
authors.affiliation: Department of Neurology, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, North Carolina 27103.
authors.forename: K S
authors.initials: KS
authors.lastname: Oles
authors.forename: J K
authors.initials: JK
authors.lastname: Penry
authors.forename: R D
authors.initials: RD
authors.lastname: Dyer
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002220:Carbamazepine / Q000031:analogs & derivatives / Q000097:blood*; D002648:Child; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique; D006801:Humans; D008875:Middle Aged; D013050:Spectrometry, Fluorescence
chemical_list: D002220:Carbamazepine; C012227:carbamazepine epoxide