Bone marrow and tissue expression of gpIIb/IIIa, LFA-1, Mac-1 and gp150,95 glycoproteins.
Monoclonal antibodies (MAbs) against platelet glycoprotein gpIIb/IIIa and the leucocyte adhesion molecules LFA-1, Mac-1, and gp 150,95 alpha chain (CD11a,b,c) and beta chain (CD18) have been tested in normal and leukaemic bone marrows, in different human tissues, and in a patient with leucocyte adhesion deficiency (LAD). The effect of these MAbs on platelet aggregation was also tested. GpIIb/IIIa showed widespread distribution, while reactivity of CD11/18 antibodies was limited to haematopoietic cells. Platelets and megakaryocytes were reactive with one CD11a (25.5.2), and with no CD11b/c or CD18 MAbs. GpIIb/IIIa was present on the platelets of the patient with LAD, whereas 25.5.2, (CD11a) bound to his platelets but not to his leucocytes. These data indicate that LFA-1, Mac-1, and gp150,95 are not present on human platelets, but they suggest the existence of crossreacting epitopes on gpIIb/IIIa, which is consistent with the hypothesis that these molecules belong to a supergene family of adhesion molecules.
Soligo
D
D
Istituto di Scienze Mediche, University of Milan, Italy.
Cattoretti
G
G
Colombi
M
M
Polli
N
N
Capsoni
F
F
Rilke
F
F
Deliliers
G L
GL
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000032:analysis*; D001853:Bone Marrow / Q000032:analysis*; D002448:Cell Adhesion; D006801:Humans; D007150:Immunohistochemistry; D016167:Integrin alphaXbeta2; D007938:Leukemia / Q000378:metabolism; D007962:Leukocytes / Q000502:physiology; D016169:Lymphocyte Function-Associated Antigen-1; D016177:Macrophage-1 Antigen; D008562:Membrane Glycoproteins / Q000032:analysis; D010974:Platelet Aggregation; D010980:Platelet Membrane Glycoproteins / Q000032:analysis*
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D016167:Integrin alphaXbeta2; D016169:Lymphocyte Function-Associated Antigen-1; D016177:Macrophage-1 Antigen; D008562:Membrane Glycoproteins; D010980:Platelet Membrane Glycoproteins