Human interleukin 7: molecular cloning and growth factor activity on human and murine B-lineage cells.
A cDNA encoding biologically active human interleukin 7 was isolated by hybridization with the homologous murine clone. Nucleotide sequence analysis indicated that this cDNA was capable of encoding a protein of 177 amino acids with a signal sequence of 25 amino acids and a calculated mass of 17.4 kDa for the mature protein. Recombinant human interleukin 7 stimulated the proliferation of murine pre-B cells and was active on cells harvested from human bone marrow that are enriched for B-lineage progenitor cells. Analysis of RNA by blot hybridization demonstrated the presence of two size classes of interleukin 7 mRNA in human splenic and thymic tissue.
Goodwin
R G
RG
Immunex Corporation, Seattle, WA 98101.
Lupton
S
S
Schmierer
A
A
Hjerrild
K J
KJ
Jerzy
R
R
Clevenger
W
W
Gillis
S
S
Cosman
D
D
Namen
A E
AE
D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D006133:Growth Substances / Q000235:genetics*; D006801:Humans; D015851:Interleukin-7; D007378:Interleukins / Q000235:genetics* / Q000494:pharmacology / Q000502:physiology; D008213:Lymphocyte Activation; D051379:Mice; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000494:pharmacology; D012689:Sequence Homology, Nucleic Acid
D006133:Growth Substances; D015851:Interleukin-7; D007378:Interleukins; D011994:Recombinant Proteins