Overproduction of SSB protein enhances the capacity for photorepair in Escherichia coli recA cells.
We studied photoreactivation in cells carrying the multicopy ssb+ plasmid. These cells overproduce single-stranded DNA-binding protein (SSB). Overproduction of SSB enhances the capacity for photoreactivation in recA bacteria but not in the recA+ background. It is suggested that, in recA cells, SSB binds to the dimer region of DNA and that this binding stimulates the process of photoreactivation. In recA+ cells, the same stimulation might be achieved by RecA protein.
Lers
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Salaj-Smic
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Trgovcević
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D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000528:radiation effects*; D008027:Light; D011938:Rec A Recombinases / Q000235:genetics*; D014466:Ultraviolet Rays
D004268:DNA-Binding Proteins; D011938:Rec A Recombinases