title: Production of IgE antibodies to mite in guinea pigs by nasal immunization.
abstract: There has been no report that IgE antibodies can be raised in guinea pigs by immunizations through respiratory tracts. We dropped a 0.1 ml mixture of 50 micrograms mite extract and aluminium hydroxide gel (Alum) into each of both nostrils of 5 guinea pigs. A mite extract alone was dropped into the nostrils of another 5 guinea pigs. The immunization was done every week. IgE antibodies were detected in one of guinea pigs sensitized 5 times with Alum + mite and also in another guinea pig in the same group sensitized 10 times. No IgE antibodies were detected in the animals immunized with mite alone.
authors.forename: A
authors.initials: A
authors.lastname: Ishii
authors.forename: K
authors.initials: K
authors.lastname: Ito
authors.forename: Y
authors.initials: Y
authors.lastname: Ino
authors.forename: S
authors.initials: S
authors.lastname: Murakami
authors.forename: T
authors.initials: T
authors.lastname: Miyamoto
mesh_terms: D000534:Alum Compounds / Q000008:administration & dosage; D000818:Animals; D000941:Antigens / Q000008:administration & dosage / Q000276:immunology; D006168:Guinea Pigs; D007114:Immunization / Q000379:methods; D007073:Immunoglobulin E / Q000096:biosynthesis*; D008297:Male; D008925:Mites / Q000276:immunology*; D009666:Nose
chemical_list: D000534:Alum Compounds; D000941:Antigens; C041524:aluminum sulfate; D007073:Immunoglobulin E