title: Evaluation of the EMIT amitriptyline and nortriptyline assays for the determination of serum clomipramine and desmethylclomipramine.
abstract: Homogeneous enzyme immunoassay reagents (EMIT) developed for the measurement of amitriptyline and nortriptyline in serum were modified to allow quantitation of clomipramine and desmethylclomipramine. The method was compared to a high-performance liquid chromatographic method. Between-run precision [coefficient of variation (CV)] for clomipramine in the EMIT assay for amitriptyline ranged from 2.6 to 3.2%. For desmethylclomipramine in the nortriptyline assay, the between-run CV ranged from 1.4 to 1.9%. Serum specimens from 43 patients (desmethylclomipramine) and 59 patients (clomipramine) were analyzed by both methods, with good correlation between methods. For clomipramine, recovery ranged from 100 to 102% (0-600 ng/ml range) and was 95-103% for desmethylclomipramine (0-600 ng/ml). The modified EMIT assays offered sufficient reproducibility, accuracy, and correlation with an established method for routine analysis of clomipramine and desmethylclomipramine.
authors.affiliation: Toxicology Laboratory, Victoria General Hospital, Halifax, Nova Scotia, Canada.
authors.forename: A D
authors.initials: AD
authors.lastname: Fraser
authors.forename: W
authors.initials: W
authors.lastname: Bryan
authors.forename: A F
authors.initials: AF
authors.lastname: Isner
mesh_terms: D000639:Amitriptyline / Q000097:blood*; D000918:Antibody Specificity; D002997:Clomipramine / Q000031:analogs & derivatives* / Q000097:blood*; D003429:Cross Reactions; D005069:Evaluation Studies as Topic; D006801:Humans; D007124:Immunoenzyme Techniques; D007202:Indicators and Reagents; D009661:Nortriptyline / Q000097:blood*
chemical_list: D007202:Indicators and Reagents; C012273:desmethylclomipramine; D000639:Amitriptyline; D009661:Nortriptyline; D002997:Clomipramine