Distribution of the beta 1 subgroup of the integrins in human cells and tissues.
We studied the distribution of the beta 1 integrin subfamily in human tissues and cells by light microscopy, electron microscopy, and immunoblotting, using monoclonal antibody DH12, previously shown to react with the beta 1 subunit of the human fibronectin receptor. Crossreaction with the other beta subunits of the integrin family, which have 45% and 47% primary amino acid sequence identity with the beta 1 subunit, was excluded, as MAb DH12 did not react with the beta 2 subunit in granulocytes and the beta 3 subunit in thrombocytes. Reactivity with the anti-beta 1 antibody was found in skin, lung, heart, striated and smooth muscle, blood cells, liver, kidney, intestine, spleen and placenta. Thus, cells of mesodermal, ectodermal, and entodermal origin express the beta 1 subunit. In skin fibroblasts cultured in vitro, beta 1 subunit was also detected intracellularly. The wide distribution of the beta 1 family, originally detected in activated T-lymphocytes after prolonged culture in vitro, contrast with the restricted distribution of the beta 2 integrins on leucocytes.
De Strooper
B
B
Center for Human Genetics, University of Leuven, Belgium.
Van der Schueren
B
B
Jaspers
M
M
Saison
M
M
Spaepen
M
M
Van Leuven
F
F
Van den Berghe
H
H
Cassiman
J J
JJ
D000911:Antibodies, Monoclonal; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005347:Fibroblasts / Q000032:analysis; D006801:Humans; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D007163:Immunosorbent Techniques; D016023:Integrins; D007422:Intestines / Q000032:analysis; D007668:Kidney / Q000032:analysis; D008099:Liver / Q000032:analysis; D008168:Lung / Q000032:analysis; D008562:Membrane Glycoproteins / Q000032:analysis*; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D010920:Placenta / Q000032:analysis; D011247:Pregnancy; D012867:Skin / Q000032:analysis; D013154:Spleen / Q000032:analysis; D014018:Tissue Distribution; D014470:Umbilical Cord / Q000032:analysis
D000911:Antibodies, Monoclonal; D016023:Integrins; D008562:Membrane Glycoproteins