RNA-catalysed synthesis of complementary-strand RNA.
The Tetrahymena ribozyme can splice together multiple oligonucleotides aligned on a template strand to yield a fully complementary product strand. This reaction demonstrates the feasibility of RNA-catalysed RNA replications.
Doudna
J A
JA
Department of Molecular Biology, Massachusetts General Hospital, Boston 02114.
Szostak
J W
JW
D000818:Animals; D001483:Base Sequence; D007438:Introns; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000378:metabolism; D012326:RNA Splicing; D016337:RNA, Catalytic; D012335:RNA, Ribosomal / Q000235:genetics / Q000378:metabolism*; D013095:Spermidine / Q000494:pharmacology; D013698:Templates, Genetic; D013768:Tetrahymena / Q000235:genetics*
D009841:Oligonucleotides; D016337:RNA, Catalytic; D012335:RNA, Ribosomal; D013095:Spermidine