Mouse hematopoietic stem-cell antigen Sca-1 is a member of the Ly-6 antigen family.
Recently, hematopoietic stem cells were purified to homogeneity from mouse bone marrow. The protein structure of Sca-1, the cell surface antigen used in the isolation of hematopoietic stem cells, is described here. It is shown that the Sca-1 antigen is a member of the Ly-6 antigen family. The anti-Sca-1 antibody was used in immunohistochemistry experiments to define the structures in several tissues that had previously been shown to contain Ly-6 antigens. In thymus, spleen, and kidney, specific staining of parenchymal cells can be demonstrated, whereas only vasculature reacts with anti-Sca-1 in brain, heart, and liver and possibly in lung.
van de Rijn
M
M
Department of Pathology, Stanford University School of Medicine, CA 94305.
Heimfeld
S
S
Spangrude
G J
GJ
Weissman
I L
IL
D000818:Animals; D000911:Antibodies, Monoclonal; D000950:Antigens, Ly / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D001853:Bone Marrow / Q000276:immunology; D004259:DNA-Directed DNA Polymerase; D005455:Fluorescent Antibody Technique; D005784:Gene Amplification; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D007124:Immunoenzyme Techniques; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014158:Transcription, Genetic
D000911:Antibodies, Monoclonal; D000950:Antigens, Ly; D012333:RNA, Messenger; D004259:DNA-Directed DNA Polymerase