Amplification of fluoroaluminate-stimulated inositol phosphate generation in a cell line overexpressing the p21N-ras gene.
The stimulation of inositol phosphate generation in NIH-3T3 cells and a derived transformant overexpressing the p21N-ras gene (T15+ cells) was examined. Incubation with NaF in the presence of Al3+ leads to the generation of inositol phosphates in each cell type, though the response in the T15+ cells is significantly amplified. The effect of fluoroaluminate is dose- and time-dependent. No differences were observed in fluoroaluminate-stimulated cyclic AMP accumulation among the cell types. In another NIH-3T3-derived cell line that expresses the transforming lys61 mutant of N-ras, no amplification of fluoroaluminate-stimulated inositol phosphate generation is observed. These results provide support for the proposal that, in the T15 cell line, p21N-ras can act in a guanine nucleotide-binding regulatory protein (G-protein)-like manner.
Wakelam
M J
MJ
Department of Biochemistry, University of Glasgow, Scotland, U.K.
D000535:Aluminum; D002460:Cell Line; D005461:Fluorine; D005784:Gene Amplification; D011905:Genes, ras; D007295:Inositol Phosphates / Q000378:metabolism*; D013403:Sugar Phosphates / Q000378:metabolism*
D007295:Inositol Phosphates; D013403:Sugar Phosphates; C053629:fluoroaluminum; D005461:Fluorine; D000535:Aluminum