Simultaneous quantification of two plant viruses by double-label time-resolved immunofluorometric assay.
For simultaneous and sensitive detection of two antigens in one sample, monoclonal antibody (MAb) to potato virus M (PVM) was labelled with a lanthanide Eu3+ and MAb to potato virus X (PVX) with another lanthanide Sm3+. A mixture of the labelled MAbs was used as a tracer. After performing the immunoreactions, the fluorescence of the dissociated lanthanides was measured at different wave-lengths in a time-resolved fluorometer to quantificate the PVX and PVM amount in a sample. Double-label time-resolved fluoroimmunoassay (TR-FIA) detected 1 ng/ml of each virus and was therefore more sensitive for simultaneous detection of PVX and PVM than reported for a single virus detection with double antibody sandwich ELISA (DAS-ELISA).
Saarma
M
M
Laboratory of Molecular Genetics, Estonian Academy of Sciences, U.S.S.R.
Järvekülg
L
L
Hemmilä
I
I
Siitari
H
H
Sinijärv
R
R
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis; D005063:Europium; D005455:Fluorescent Antibody Technique; D010942:Plant Viruses / Q000276:immunology / Q000302:isolation & purification*; D012493:Samarium; D014773:Virology / Q000379:methods
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D012493:Samarium; D005063:Europium