Assessment of a self-contained solid phase enzyme linked immunoassay (ImmunoComb) for the determination of serum total IgE.
A semiquantitative method (ImmunoComb) for measuring total serum IgE is described and compared with a standard radioimmunoassay (PRIST). ImmunoComb, based on a solid-phase enzyme-linked immunoassay, is self-sufficient and does not require any expensive laboratory equipment. Comparison of these two assays revealed an almost absolute agreement between them (correlation coefficient = 0.95). Furthermore, the ImmunoComb assay also demonstrated its rapidity and temperature independence while maintaining a recovery of 90% to 102%.
Fink
A
A
Clinical Laboratories, Kaplan Hospital, Rehovot, Israel.
Dvir
G
G
Miskin
A
A
D005069:Evaluation Studies as Topic; D006801:Humans; D007124:Immunoenzyme Techniques; D007073:Immunoglobulin E / Q000032:analysis*; D013696:Temperature
D007073:Immunoglobulin E