Expression of the plum pox virus coat protein region in Escherichia coli.
A cDNA complementary to the 3' end of plum pox virus (PPV) RNA was sequenced. The sequence was investigated for the presumable coat protein cistron by computer-aided translation. A fragment containing the stop codon of the polyprotein gene and a putative virus-specific protease cleavage site was subcloned into an E. coli expression vector. It is shown by immunological analysis that the coat protein cistron is located within the subcloned region.
Mattanovich
D
D
Institute for Applied Microbiology, University of Agriculture, Vienna, Austria.
Himmler
G
G
Laimer
M
M
Maiss
E
E
Regner
F
F
Da Camara Machado
A
A
Hanzer
V
V
Casper
R
R
Katinger
H W
HW
D000595:Amino Acid Sequence; D001483:Base Sequence; D015153:Blotting, Western; D002213:Capsid / Q000096:biosynthesis / Q000235:genetics*; D003001:Cloning, Molecular; D001330:Electronic Data Processing; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D005814:Genes, Viral; D008969:Molecular Sequence Data; D010942:Plant Viruses / Q000235:genetics*; D010957:Plasmids; D012367:RNA, Viral / Q000235:genetics; D015183:Restriction Mapping
D012367:RNA, Viral