Lipopolysaccharide-induced insulin resistance in monolayers of cultured hepatocytes.
In order to clarify the endotoxin effect on the hepatic removal of insulin, the influence of lipopolysaccharide (LPS) from E. coli 0111:B4 on the insulin binding and endocytosis in cultured hepatocytes from adult male rats has been investigated. LPS decreases both processes in a time and temperature-dependent manner, showing a major effect at short time and low temperature, according to the characteristics of LPS binding and uptake.
Portolés
M T
MT
Department of Biochemistry & Molecular Biology I, Faculty of Chemistry, Universidad Complutense, Madrid, Spain.
Pagani
R
R
Ainaga
M J
MJ
Díaz-Laviada
I
I
Municio
A M
AM
D000818:Animals; D002478:Cells, Cultured; D003080:Cold Temperature; D004926:Escherichia coli; D007333:Insulin Resistance; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008099:Liver / Q000166:cytology / Q000187:drug effects*; D008297:Male; D051381:Rats; D013997:Time Factors
D008070:Lipopolysaccharides