[Mutagenesis directed by phosphotriester analogs of oligonucleotides: a way to site-specific mutagenesis in vivo].
A new approach is proposed to obtain the directed mutations in the gene under study. The technique is based on using alkylphosphotriester analogues of oligodeoxyribonucleotides as site-specific mutagens. The deletion C in lacZ' gene of bacteriophage M13mpB was obtained by cotransfection of Escherichia coli cells with a mix of DNA and phosphotriester analogues of oligonucleotides.
Petrenko
V A
VA
Kipriianov
S M
SM
Boldyrev
A N
AN
Pozdniakov
P I
PI
D001483:Base Sequence; D003090:Coliphages / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D009841:Oligonucleotides / Q000235:genetics*; D013698:Templates, Genetic; D014162:Transfection; D014170:Transformation, Genetic
D009841:Oligonucleotides
Mutagenez, napravlennyĭ fosfotriéfirnymi analogami oligonukleotidov, - put' k saĭt-spetsificheskomu mutagenezu in vivo.