Amino acid sequence of a 15 kilodalton actin-binding fragment of turkey gizzard caldesmon: similarity with dystrophin, tropomyosin and the tropomyosin-binding region of troponin T.
We have determined the amino acid sequence of a 15 kDa actin-binding fragment of turkey gizzard caldesmon. The 96-residue fragment contains 29 acidic and 29 basic residues, and is predicted to have an extended helical conformation stabilized by numerous internal salt bridges. CaD15 bears some resemblance to dystrophin, tropomyosin and several other proteins, but is most strikingly similar to the tropomyosin-binding segment of troponin T.
Leszyk
J
J
Department of Biological Chemistry, School of Medicine, University of Maryland, Baltimore 21201.
Mornet
D
D
Audemard
E
E
Collins
J H
JH
D000199:Actins / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002148:Calmodulin-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D016189:Dystrophin; D005895:Gizzard, Avian / Q000032:analysis*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009124:Muscle Proteins; D010446:Peptide Fragments / Q000302:isolation & purification / Q000378:metabolism; D011487:Protein Conformation; D012689:Sequence Homology, Nucleic Acid; D014335:Tropomyosin / Q000378:metabolism; D014336:Troponin / Q000378:metabolism; D020107:Troponin T; D014422:Turkeys
D000199:Actins; D002148:Calmodulin-Binding Proteins; D016189:Dystrophin; D009124:Muscle Proteins; D010446:Peptide Fragments; D014335:Tropomyosin; D014336:Troponin; D020107:Troponin T