title: Simple three-dimensional imaging of HRP-labelled neurons with the aid of an image processor.
abstract: A method is described for visualizing the three-dimensional structure of horseradish peroxidase-labelled neurons in the central nervous system of Xenopus laevis tadpoles. The reconstruction is simple, and performed on a commercially available image processing system. Minimal operational skill is required for using this method. Also, various improvements were made where user's simplicity (user-friendliness), speed and required computer memory are concerned. In addition, the excellent properties of the IBAS II system, such as image filtering and image editing functions are fully available, offering a fast, clean and complete final image reconstruction.
authors.affiliation: Department of Anatomy and Embryology, University of Nijmegen, The Netherlands.
authors.forename: J A
authors.initials: JA
authors.lastname: van der Linden
authors.forename: M
authors.initials: M
authors.lastname: Terlou
authors.forename: M
authors.initials: M
authors.lastname: Kramer
mesh_terms: D000465:Algorithms; D000818:Animals; D001921:Brain / Q000166:cytology*; D003196:Computer Graphics; D003201:Computers; D006735:Horseradish Peroxidase; D007091:Image Processing, Computer-Assisted; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D009474:Neurons / Q000648:ultrastructure*; D014982:Xenopus laevis
chemical_list: D006735:Horseradish Peroxidase