[Analysis of point mutations of Ha-ras gene in basaliomas and spinaliomas usiang the polymerase chain reaction (PCR) technic].
Polymerase chain reaction is a recently developed procedure to detect point mutations in human genomic DNA. The sensitivity and specificity of this technique which requires a very little amount of DNA, have allowed the large scale screening of tumors. The Authors report the detection of point mutations of Ha-ras gene at codon 12 in 12 basal cell carcinomas and 10 cutaneous squamous cell carcinomas by the use of polymerase chain reaction in paraffin-embedded tissue sections. The gene alteration was identified in 2 basaliomas whereas no squamous cell carcinoma showed point mutations. These results suggest that point mutations of Ha-ras gene do not represent a general activating mechanism in cutaneous epitheliomas.
Peris
K
K
Cerroni
L
L
Chimenti
S
S
Soyer
H P
HP
Kerl
H
H
Höfler
H
H
D002280:Basal Cell Carcinoma / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D003062:Codon; D006801:Humans; D009154:Mutation; D016133:Polymerase Chain Reaction; D012878:Skin Neoplasms / Q000235:genetics*
D003062:Codon
Analisi delle mutazioni puntiformi del gene Ha-ras in basaliomi e spinaliomi mediante la technica della polymerase chain reaction (PCR).