The effect of vitamin B2 and its derivatives on a dry-reagent strip theophylline assay.
This report describes the effect of flavin adenine dinucleotide (FAD) and its precursor vitamin B2 on an apoenzyme reactivation immunoassay system (ARIS) both in vitro and in vivo. It was observed that 10 nM/L FAD in the buffer causes a false-positive result in ARIS for theophylline by the value of 94 microM/L, whereas vitamin B2, flavin mononucleotide, adenosine, adenosine monophosphate, and adenosine diphosphate (ADP) at the same concentration caused no such effect. Nevertheless, ADP at 2 microM/L can cause a drop in theophylline reading of 55 microM/L. However, the sera from four volunteers with normal liver functions who had taken 30 mg of vitamin B2 daily for a week showed no effect on theophylline reading.
Wei
J S
JS
Department of Biochemistry, Chang Gung Medical College, Taoyuan, Taiwan, Republic of China.
Tsai
C Y
CY
Huang
H M
HM
Chai
M E
ME
D005189:False Positive Reactions; D005182:Flavin-Adenine Dinucleotide / Q000494:pharmacology*; D006801:Humans; D007124:Immunoenzyme Techniques; D011934:Reagent Strips; D013806:Theophylline / Q000097:blood*; D014805:Vitamin B 12 / Q000494:pharmacology*
D011934:Reagent Strips; D005182:Flavin-Adenine Dinucleotide; D013806:Theophylline; D014805:Vitamin B 12