Supercoil-induced Z-DNA formation within 5'-end of chicken myb proto-oncogene.
We have analyzed the recently sequenced and characterized 2.9 kb fragment derived from the 5'-end of chicken myb proto-oncogene with respect to structural perturbations induced by DNA supercoiling. Within the first intron a 50 bp sequence stretch was localized, starting approximately 450 nucleotides downstream from putative ATG initiation codon, which forms a non-B-DNA structure. Fine mapping with structural probes revealed the three adjacent regions with imperfect purine-pyrimidine alternation creating together relatively long Z-forming tract, parts of which may undergo a B-Z DNA transition at different superhelical densities.
Vlach
J
J
Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, Prague.
Dvorak
M
M
Bartunek
P
P
Pecenka
V
V
Travnicek
M
M
Sponar
J
J
D000478:Alkylation; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D003001:Cloning, Molecular; D003062:Codon; D004278:DNA, Superhelical; D004047:Diethyl Pyrocarbonate; D004587:Electrophoresis, Agar Gel; D007438:Introns; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009993:Osmium Tetroxide; D010957:Plasmids; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D020598:Proto-Oncogene Proteins c-myb
D003062:Codon; D004278:DNA, Superhelical; D011518:Proto-Oncogene Proteins; D020598:Proto-Oncogene Proteins c-myb; D004047:Diethyl Pyrocarbonate; D009993:Osmium Tetroxide