Stimulus-secretion coupling of arginine-induced insulin release. Insulinotropic action of agmatine.
Agmatine causes a concentration-related (0.1-1.0 mM) stimulation of insulin secretion by rat pancreatic islets exposed to D-glucose (5.6-11.1 mM) but, like L-arginine, fails to affect insulin release either in the absence of glucose or at a high concentration (16.7 mM) of the hexose. Agmatine, which accumulates in islet cells, also stimulates 45Ca net uptake by the islets. The secretory response to agmatine represents a delayed process. It is enhanced by theophylline and suppressed by antimycin A or the absence of extracellular Ca2+. The insulinotropic action of agmatine is compatible with the view that endogenously formed polyamines may play a role in the secretory response of islet cells to L-arginine.
Sener
A
A
Laboratory of Experimental Medicine, Brussels Free University, Belgium.
Lebrun
P
P
Blachier
F
F
Malaisse
W J
WJ
D000376:Agmatine / Q000494:pharmacology*; D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D001120:Arginine / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D006146:Guanidines / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D051381:Rats; D012636:Secretory Rate / Q000187:drug effects; D013806:Theophylline / Q000494:pharmacology; D013997:Time Factors
D006146:Guanidines; D007328:Insulin; D000968:Antimycin A; D000376:Agmatine; D001120:Arginine; D013806:Theophylline; D005947:Glucose; D002118:Calcium