A 10 kDa polypeptide associated with the oxygen-evolving complex of photosystem II has a putative C-terminal non-cleavable thylakoid transfer domain.
The N-terminal amino acid sequence of the 10 kDa polypeptide associated with the oxygen-evolving complex of wheat photosystem II has been determined and shown to be homologous to the amino acid sequence of the product of the ST-LS1 gene from potato. The N-terminal sequence of the mature protein indicates that the polypeptide is synthesized with a 39 amino acid N-terminal presequence which is similar to chloroplast import sequences but which lacks a hydrophobic domain for transfer of the protein across the thylakoid membrane. The mature polypeptide has a C-terminal hydrophobic region which shows homology to the hydrophobic thylakoid transfer domain of other lumenal proteins and this hydrophobic region of the 10 kDa polypeptide is suggested to facilitate transfer of the protein across the thylakoid membrane.
Webber
A N
AN
Department of Botany, University of Cambridge, England.
Packman
L C
LC
Gray
J C
JC
D000595:Amino Acid Sequence; D002734:Chlorophyll / Q000032:analysis*; D045342:Light-Harvesting Protein Complexes; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010100:Oxygen / Q000378:metabolism; D010788:Photosynthesis; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D010940:Plant Proteins / Q000032:analysis*; D012995:Solubility; D014908:Triticum
D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D002734:Chlorophyll; D010100:Oxygen