Amino-terminal region of human macrophage colony-stimulating factor (M-CSF) is sufficient for its in vitro biological activity: molecular cloning and expression of carboxyl-terminal deletion mutants of human M-CSF.
Human T lymphoblastoid cell line CEM-ON belongs to a helper/inducer subclass and secretes M-CSF into medium constitutively. We have isolated a full-length cDNA clone for this factor from a cDNA library of this cell line. The cDNA was 2.5 kb and coded for a 554 amino acid polypeptide precursor including signal sequence. The Northern blot analysis showed that the major transcript of M-CSF is about 4.2 kb. We have studied the expression of not only the wild type plasmid, but also the five C-terminal deletion mutants encoding N-terminal 154, 163, 170, 177, and 185 amino acid residues in monkey COS-1 cells. The results showed that at least C-terminal 377 amino acid residues of human M-CSF are not essential to manifest the in vitro biological activity on murine bone marrow cells.
Takahashi
M
M
Cellular Technology Institute, Otsuka Pharmaceutical, Co., Ltd. Tokushima, Japan.
Hirato
T
T
Takano
M
M
Nishida
T
T
Nagamura
K
K
Kamogashira
T
T
Nakai
S
S
Hirai
Y
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D003115:Colony-Stimulating Factors / Q000235:genetics / Q000494:pharmacology*; D004252:DNA Mutational Analysis; D006801:Humans; D016173:Macrophage Colony-Stimulating Factor; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000494:pharmacology; D013329:Structure-Activity Relationship
D003115:Colony-Stimulating Factors; D011994:Recombinant Proteins; D016173:Macrophage Colony-Stimulating Factor