Investigation of a simple amperometric electrode system to rapidly quantify and detect bacteria in foods.
A two electrode system mounted as a single probe was developed to measure electrochemically the rate of reduction of a redox mediator (thionine) by bacteria. The system gave a rapid (2 min) bacterial-dependent current above 2.5 x 10(5) cfu/ml with pure cultures of bacteria, but when applied to the measurement of the bacterial contamination in samples of meat and milk it was unable to detect or quantify the contamination reliably. Incubation of samples for a few hours before examination enabled the system to detect bacteria in excess of 10(6) cfu/ml.
Patchett
R A
RA
Department of Microbiology, AFRC Institute of Food Research, Reading Laboratory, Shinfield, UK.
Kelly
A F
AF
Kroll
R G
RG
D000818:Animals; D001419:Bacteria / Q000302:isolation & purification*; D001431:Bacteriological Techniques; D015169:Colony Count, Microbial; D004553:Electric Conductivity; D004566:Electrodes; D005516:Food Microbiology; D008460:Meat; D008892:Milk / Q000382:microbiology; D010084:Oxidation-Reduction