title: Isolation and partial characterization of a cytochrome-o complex from chromatophores of the photosynthetic bacterium Rhodospirillum rubrum FR1.
abstract: A cytochrome-o complex was isolated from chromatophores of photoheterotrophically grown Rhodospirillum rubrum FR1. The enzyme was extracted with the non-denaturating detergent taurodeoxycholate and subsequently purified by sucrose-density-gradient centrifugation and gel-permeation HPLC. The complex contains two types of cytochromes, one of them cytochrome o, and two copper atoms. It catalyzes the reduction of molecular oxygen, when N,N,N',N'-tetramethyl-p-phenylenediamine or ubiquinol 10 are offered as electron donors. The oxidase activity is inhibited by cyanide, carbon monoxide and 2-heptyl-2-hydroxyquinoline N-oxide. The molecular mass of the protein is 136 +/- 15 kDa. The subunit analysis, by SDS continuous and gradient gels, revealed four subunits with molecular mass 66 kDa (subunit I), 36 kDa (subunit II), 20 kDa (subunit III) and 11 kDa (subunit IV).
authors.affiliation: Institut für Biochemie, Johannes Gutenberg Universität, Mainz, Federal Republic of Germany.
authors.forename: A S
authors.initials: AS
authors.lastname: Schrattenholz
authors.forename: T
authors.initials: T
authors.lastname: Nawroth
authors.forename: K
authors.initials: K
authors.lastname: Dose
mesh_terms: D020130:Bacterial Chromatophores / Q000201:enzymology*; D001426:Bacterial Proteins / Q000302:isolation & purification*; D003573:Cytochrome b Group; D003580:Cytochromes / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D004227:Dithionite / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D029968:Escherichia coli Proteins; D045322:Photosynthetic Reaction Center Complex Proteins; D012246:Rhodospirillum / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D003573:Cytochrome b Group; D003580:Cytochromes; D029968:Escherichia coli Proteins; D045322:Photosynthetic Reaction Center Complex Proteins; D004227:Dithionite; C017168:cytochrome bo, E coli