RNA transfection of Escherichia coli by electroporation.
Cells of Escherichia coli were efficiently transfected with Q beta phage RNA by electroporation. A single voltage shock at 6.25 kV/cm with a 25 microF capacitor resulted in an infectivity yield considerably higher than that attained by a lysozyme-EDTA spheroplast method or a CaCl2 procedure. A linear relationship was found between concentration of the input RNA and yield of the transfectants, over a wide range. Efficiency of the electroporation-mediated transfection (electrotransfection) was increased by addition of certain sodium salts but decreased by preincubation in a Tris buffer containing sucrose.
Taketo
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Department of Biochemistry, Fukui Medical School, Japan.
D001435:Bacteriophages / Q000235:genetics; D002122:Calcium Chloride / Q000494:pharmacology; D002463:Cell Membrane Permeability; D004558:Electric Stimulation; D004926:Escherichia coli / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D013104:Spheroplasts; D014162:Transfection
D012367:RNA, Viral; D002122:Calcium Chloride