Cyanogen bromide cleavage at methionine residues of polypeptides containing disulfide bonds.
A method for cleaving polypeptides at their methionine residues without affecting intramolecular disulfide bonds is described. This method may be applied for cleaving recombinant heterologous hybrid polypeptides with release of the interesting peptide. The method may also be applied to assign the correct positions of disulfide bonds in protein molecules.
Villa
S
S
R-DNA Department, Instituto di Ricerca Cesare Serono, Ardea, Italy.
De Fazio
G
G
Canosi
U
U
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003488:Cyanogen Bromide; D004220:Disulfides; D004926:Escherichia coli / Q000032:analysis; D005561:Formates; D019791:Guanidine; D006146:Guanidines; D008715:Methionine; D010084:Oxidation-Reduction; D010446:Peptide Fragments / Q000032:analysis*; D010455:Peptides; D011994:Recombinant Proteins
D004220:Disulfides; D005561:Formates; D006146:Guanidines; D010446:Peptide Fragments; D010455:Peptides; D011994:Recombinant Proteins; C030544:formic acid; D008715:Methionine; D019791:Guanidine; D003488:Cyanogen Bromide