title: Three-dimensional imaging in fluorescence by confocal scanning microscopy.
abstract: The improved resolution and sectioning capability of a confocal microscope make it an ideal instrument for extracting three-dimensional information especially from extended biological specimens. The imaging properties, also with finite detection pinholes are considered and a number of biological applications demonstrated.
authors.affiliation: Department of Electrical Microscopy and Molecular Cytology, University of Amsterdam, The Netherlands.
authors.forename: G J
authors.initials: GJ
authors.lastname: Brakenhoff
authors.forename: H T
authors.initials: HT
authors.lastname: van der Voort
authors.forename: E A
authors.initials: EA
authors.lastname: van Spronsen
authors.forename: N
authors.initials: N
authors.lastname: Nanninga
mesh_terms: D000818:Animals; D002696:Chlamydomonas / Q000648:ultrastructure; D007091:Image Processing, Computer-Assisted; D007834:Lasers; D008433:Mathematics; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D014411:Neoplastic Stem Cells; D010944:Plants / Q000648:ultrastructure; D012441:Saccharomyces cerevisiae / Q000032:analysis; D014407:Tumor Cells, Cultured