title: SIS/PDGF-B expression in benign and malignant human breast lesions.
abstract: Northern blots with poly(A)-selected RNAs were prepared from four primary human breast carcinomas and hybridized with a SIS/PDGF-B gene probe. High expression of a normal-sized 3.7 kb SIS/PDGF-B transcript was observed in three samples. These three carcinomas were all T3 (greater than 5 cm) lesions and were histologically infiltrating ductal carcinomas accompanied by moderate to marked desmoplasia. Furthermore in situ hybridization with photobiotinylated probes was performed and demonstrated that the SIS/PDGF-B expression was localized within the epithelial cells of malignant as well as benign lesions. It is possible that SIS/PDGFB expression within the epithelial cell components of nonmalignant as well as malignant breast lesions may be in part responsible for the stromal reaction.
authors.affiliation: Department of Medical Oncology, University of Texas, M.D. Anderson Cancer Center, Houston 77030.
authors.forename: J
authors.initials: J
authors.lastname: Ro
authors.forename: J
authors.initials: J
authors.lastname: Bresser
authors.forename: J Y
authors.initials: JY
authors.lastname: Ro
authors.forename: F
authors.initials: F
authors.lastname: Brasfield
authors.forename: G
authors.initials: G
authors.lastname: Hortobagyi
authors.forename: M
authors.initials: M
authors.lastname: Blick
mesh_terms: D015152:Blotting, Northern; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology; D004848:Epithelium / Q000502:physiology; D005786:Gene Expression Regulation; D006801:Humans; D009693:Nucleic Acid Hybridization; D010982:Platelet-Derived Growth Factor / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D010982:Platelet-Derived Growth Factor; D011518:Proto-Oncogene Proteins; D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger