Characterization of antibody against human liver guanase by immunoblotting and immunohistochemical staining of human liver guanase by a direct labeling antibody-enzyme method.
Human liver guanase was purified and a specific antibody against it was raised in rabbits. The antiserum formed a single precipitin line with human liver extract, and also completely inhibited the activity of the liver enzyme. An immunoblotting study showed that the antibody bound specifically to one band of protein with guanase activity and not to other proteins. Therefore, we concluded that this antiserum against the liver enzyme was suitable for use in immunohistochemical demonstration of guanase. In tissue sections, the immunohistochemical reaction with this antibody was positive in the same locations as the histochemical guanase reaction with DAB (3,3'-diaminobenzidine tetrahydrochloride).
Ito
S
S
Second Department of Internal Medicine, School of Medicine, University of Tokushima, Japan.
Iwasaki
A
A
Syundo
J
J
Tamura
Y
Y
Kishi
S
S
Mori
H
H
Ii
K
K
Matsuda
Y
Y
Katsunuma
N
N
D000619:Aminohydrolases / Q000276:immunology*; D000906:Antibodies / Q000032:analysis* / Q000276:immunology; D000918:Antibody Specificity; D003429:Cross Reactions; D006148:Guanine Deaminase / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry / Q000379:methods; D008099:Liver / Q000166:cytology / Q000201:enzymology*
D000906:Antibodies; D000619:Aminohydrolases; D006148:Guanine Deaminase