Adrenocorticoid action in the spinal cord: some unique molecular properties of glucocorticoid receptors.
1. Glucocorticoid hormones affect several functions of the spinal cord, such as synaptic transmission, biogenic amine content, lipid metabolism, and the activity of some enzymes (ornithine decarboxylase, glycerolphosphate dehydrogenase), indicating that this tissue is a target of adrenal hormones. 2. Corticosterone, the main glucocorticoid of the rat, is detected at all regional levels of the spinal cord, and cold stress increases this steroid, predominantly in the cervical regions. 3. Intracellular glucocorticoid receptors have been found in the spinal cord, with higher concentrations in the cervical and lumbar enlargements. Prima facie, these receptors presented biochemical, stereospecifical, and physicochemical properties similar to those of receptors found in other regions of the nervous system. The prevalent form in the spinal cord is the type II receptor, although type I is also present in small amounts. 4. The type II glucocorticoid receptor of the spinal cord shows an affinity lower (Kd 3.5 nM) than that of the hippocampal type II site (Kd 0.7 nM) when incubated with [3H]dexamethasone. This condition may impair the nuclear translocation of the spinal cord receptor. 5. Another peculiar property of spinal cord type II site is a greater affinity for DNA-cellulose binding than the hippocampal receptor during heat-induced transformation. Also, the spinal cord receptor shows resistance to the action of RNAse A, an enzyme which increases DNA-cellulose binding of the hippocampal receptor, indicating that both receptors may be structurally different. 6. Therefore, it is possible that a different subclass of type II, or "classical glucocorticoid receptor," is present in the spinal cord. This possibility makes the cord a useful system for studying diversity of glucocorticoid receptors of the nervous system, especially the relationship between receptor structure and function.
De Nicola
A F
AF
Laboratorio de Bioquímica Neuroendócrina, Instituto de Biología y Medicina Experimental, Buenos Aires, Argentina.
Moses
D F
DF
González
S
S
Ortí
E
E
D000818:Animals; D001921:Brain / Q000378:metabolism; D003345:Corticosterone / Q000032:analysis / Q000502:physiology; D003907:Dexamethasone / Q000032:analysis / Q000378:metabolism; D005938:Glucocorticoids / Q000032:analysis / Q000494:pharmacology / Q000502:physiology*; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000032:analysis / Q000502:physiology*; D012260:Ribonucleases / Q000502:physiology; D013116:Spinal Cord / Q000378:metabolism*; D013312:Stress, Physiological / Q000378:metabolism
D005938:Glucocorticoids; D011965:Receptors, Glucocorticoid; D003907:Dexamethasone; D012260:Ribonucleases; D003345:Corticosterone