Identification and genetic mapping of the structural gene for an essential Escherichia coli membrane protein.
Attempts to isolate conditionally lethal recB and recC mutations of Escherichia coli K-12 by P1 localized mutagenesis led to the identification of the structural gene for an essential membrane protein. Located on a 1.5-kilobase-pair DNA fragment which physically mapped immediately 5' to the thyA gene, the product of the umpA (unidentified membrane protein) gene is a 25,000 Mr membrane-associated polypeptide. These results provide an explanation for why several research groups have been unable to obtain chromosomal deletions of the entire thyA gene. A possible interaction between the umpA and thyA genes is also discussed.
Williams
M G
MG
Department of Genetics, University of Georgia, Athens 30602.
Fortson
M
M
Dykstra
C C
CC
Jensen
P
P
Kushner
S R
SR
D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D043211:Exodeoxyribonuclease V; D005090:Exodeoxyribonucleases / Q000235:genetics; D005796:Genes; D005798:Genes, Bacterial; D005804:Genes, Lethal; D005816:Genetic Complementation Test; D008565:Membrane Proteins / Q000235:genetics*; D009154:Mutation; D010957:Plasmids; D014161:Transduction, Genetic
D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D005090:Exodeoxyribonucleases; D043211:Exodeoxyribonuclease V; C468322:exodeoxyribonuclease V, E coli