DNA binding specificity of the Arc and Mnt repressors is determined by a short region of N-terminal residues.
The Arc and Mnt repressors of phage P22 are related proteins that bind to different operator DNA sites. By creating a hybrid Arc-Mnt protein, we show that the binding specificity of Mnt can be switched to that of Arc by replacing six residues at the N terminus of Mnt with the corresponding nine residues from Arc.
Knight
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Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Sauer
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RT
D000595:Amino Acid Sequence; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D005814:Genes, Viral; D008969:Molecular Sequence Data; D010957:Plasmids; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D012475:Salmonella / Q000235:genetics; D012483:Salmonella Phages / Q000235:genetics*; D013379:Substrate Specificity; D014157:Transcription Factors / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism; D054334:Viral Regulatory and Accessory Proteins
D004268:DNA-Binding Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; C016649:phage repressor proteins