Granulocyte-macrophage colony-stimulating factor (CSF) and multilineage CSF recruit human monocytes to express granulocyte CSF.
We assessed the capacity of recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF) and multilineage (Multi)-CSF to induce release of granulocyte-CSF (G-CSF) by highly purified peripheral blood monocyte (Mo) preparations. Our results reveal that GM-CSF and Multi-CSF, either alone or in a synergistic concert, activate Mo to transcribe G-CSF messenger (m) RNA and release biologically active G-CSF protein into their culture supernatants. G-CSF had no regulatory effect on Mo expression of cytoplasmic G-CSF mRNA levels and G-CSF protein secretion by itself. These differential actions of CSFs provide further insight into self-regulatory mechanisms within the growth factor hierarchy system.
Oster
W
W
Department of Hematology, Johannes Gutenberg University, Mainz, FRG.
Lindemann
A
A
Mertelsmann
R
R
Herrmann
F
F
D002474:Cell-Free System; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000494:pharmacology* / Q000502:physiology; D004338:Drug Combinations; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006098:Granulocytes / Q000502:physiology*; D006133:Growth Substances / Q000494:pharmacology* / Q000502:physiology; D006801:Humans; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D012333:RNA, Messenger / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000494:pharmacology
D003115:Colony-Stimulating Factors; D004338:Drug Combinations; D006133:Growth Substances; D012333:RNA, Messenger; D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor