The effect of anti-interleukin 2 monoclonal antibody treatment on the survival of rat cardiac allograft.
The effect of anti-interleukin 2 monoclonal antibody (anti-IL2 MoAb) and the accumulation of intravenously administered 125I-labeled anti-IL2 MoAb were examined in heterotopic rat cardiac allografts. Mouse anti-human recombinant IL2 MoAb was obtained by the hybridoma technique. The anti-IL2 MoAb, termed 8H-10, was an IgG2a which inhibited IL2-driven [3H]TdR incorporation in cytolytic T lymphocyte line cells at a dilution of 2(6). 8H-10 was injected iv at a dose of 200 micrograms/day for 8 consecutive days, beginning on the day of transplantation. Hearts from F344 rats (RT11v1) were transplanted into ACI recipient rats (RT1av1). The mean survival time was 7.6 +/- 0.8 days in untreated controls, 9.0 +/- 1.2 days in additional controls treated with mouse anti-sheep red blood cell monoclonal antibody, and 25.3 +/- 18.4 days in the anti-IL2 MoAb (8H-10)-treated group (P less than 0.05). Furthermore, the accumulation of intravenously administered 125I-labeled anti-IL2 MoAb (8H-10) was specifically seen in the grafted heart. In conclusion, these results suggest that IL2 may play an important role in allograft rejection and that anti-IL2 MoAb may serve as a useful immunosuppressive agent in clinical transplantation.
Sakagami
K
K
First Department of Surgery, Okayama University Medical School, Japan.
Ohsaki
T
T
Ohnishi
T
T
Saito
S
S
Matsuoka
J
J
Orita
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use; D006082:Graft Enhancement, Immunologic / Q000379:methods*; D006084:Graft Rejection; D006321:Heart / Q000000981:diagnostic imaging; D016027:Heart Transplantation; D007376:Interleukin-2 / Q000276:immunology*; D007457:Iodine Radioisotopes; D008297:Male; D011863:Radioimmunoassay; D011877:Radionuclide Imaging; D051381:Rats; D011912:Rats, Inbred ACI; D011916:Rats, Inbred F344
D000911:Antibodies, Monoclonal; D007376:Interleukin-2; D007457:Iodine Radioisotopes