Induction of kinetochore positive and negative micronuclei in V79 cells by the alkylating agent diethylsulphate.
The induction by diethylsulphate of micronuclei derived from acentric fragments or from whole chromosomes was studied in Chinese hamster V79 cells using autoantibodies from the serum of a scleroderma patient (CREST-syndrome) to detect centromere--kinetochore structures. Centromere-containing micronuclei appeared early after treatment and plateaued both earlier and at lower level than centromere-lacking micronuclei. The frequency of centromere-containing micronuclei was in good agreement with that of mitotic chromosome displacement, suggesting that a high proportion of displaced chromosomes were transmitted to the cytoplasm of one of the two daughter cells, where they gave rise to micronuclei. On the contrary, centromere-lacking micronuclei were more frequent than what could be expected from chromosome fragments observed in mitotic stages.
Nüsse
M
M
Gesellschaft für Strahlen- und Umweltforschung, Institut für Biophysikalische Strahlenforschung, Frankfurt, FRG.
Viaggi
S
S
Bonatti
S
S
D000477:Alkylating Agents / Q000633:toxicity*; D000818:Animals; D000974:Antibodies, Antinuclear; D002455:Cell Division; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects / Q000648:ultrastructure; D004260:DNA Repair / Q000187:drug effects*; D015162:Micronucleus Tests; D009153:Mutagens; D013463:Sulfuric Acid Esters / Q000633:toxicity*; D013464:Sulfuric Acids / Q000633:toxicity*
D000477:Alkylating Agents; D000974:Antibodies, Antinuclear; D009153:Mutagens; D013463:Sulfuric Acid Esters; D013464:Sulfuric Acids; C011612:diethyl sulfate