Nucleotide sequence of the F plasmid transfer gene, traH: identification of a new gene and a promoter within the transfer operon.
The nucleotide sequence of the F plasmid transfer gene traH, which is involved in F-pilus assembly in Escherichia coli K-12, has been determined. From the sequence data, it would appear that traH encodes a 38,897-dalton precursor polypeptide which is processed to give a periplasmic protein. Furthermore, a new gene, trbF, has been located immediately upstream of traH and shown to be expressed by means of a translational fusion to lacZ. Using galK fusion and S1 nuclease protection studies, a weak traJ-dependent promoter, P trbF, has been mapped upstream and adjacent to trbF. Transcription of trbF and traH from P trbF may well serve to complement transcription from the major tra operon promoter PY located some 16 kb upstream of these genes.
Ham
L M
LM
Department of Microbiology, Monash University, Clayton, Victoria, Australia.
Firth
N
N
Skurray
R
R
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003470:Culture Media; D004926:Escherichia coli / Q000235:genetics; D005144:F Factor; D005686:Galactokinase / Q000378:metabolism; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009876:Operon; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D015183:Restriction Mapping; D014158:Transcription, Genetic
D003470:Culture Media; D005686:Galactokinase