Growth factors and hormones which affect survival, growth, and differentiation of the MCF-7 stem cells and their descendants.
The human breast tumor cell line was separated by Percoll density gradient centrifugation into six different subpopulations, A to F, one of which (E) appears to contain the stem cells on the basis of several criteria (M. Resnicoff et al. 1987, Proc. Natl. Acad. Sci. USA 84, 7295. We now analyzed the response of the isolated subpopulations to insulin, thrombin, PGF2 alpha, estradiol, and 13-cis-retinal. We demonstrate that the first two growth factors stimulate [3H]thymidine incorporation in the more differentiated subpopulations (D and F), while PGF2 alpha has mitogenic activity in subpopulations C and D. In the absence of any added growth factor, estradiol has the extreme and transient capacity of allowing the stem cell to detach from the tissue culture dish and to grow in suspension as multicellular aggregates (MCF-7/SE cells). 13-cis-Retinal acts as a negative modulator of differentiation and protects the cells from the inhibitory and differentiation activity of Na-butyrate.
Resnicoff
M
M
Instituto de Investigaciones Bioquimicas Fundacion Campomar, Buenos Aires, Argentina.
Medrano
E E
EE
D001943:Breast Neoplasms; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D015237:Dinoprost / Q000494:pharmacology*; D004224:Diterpenes; D004958:Estradiol / Q000494:pharmacology; D006728:Hormones / Q000494:pharmacology*; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D014411:Neoplastic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D012172:Retinaldehyde / Q000494:pharmacology*; D012176:Retinoids / Q000494:pharmacology*; D013917:Thrombin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D002087:Butyrates; D003470:Culture Media; D004224:Diterpenes; D006728:Hormones; D007328:Insulin; D012176:Retinoids; D020148:Butyric Acid; C031338:13-cis-retinal; D004958:Estradiol; D004247:DNA; D015237:Dinoprost; D013917:Thrombin; D012172:Retinaldehyde