Evidence for function of the ferredoxin/thioredoxin system in the reductive activation of target enzymes of isolated intact chloroplasts.
Results obtained with isolated intact chloroplasts maintained aerobically under light and dark conditions confirm earlier findings with reconstituted enzyme assays and indicate that the ferredoxin/thioredoxin system functions as a light-mediated regulatory thiol chain. The results were obtained by application of a newly devised procedure in which a membrane-permeable thiol labeling reagent, monobromobimane (mBBr), reacts with sulfhydryl groups and renders the derivatized protein fluorescent. The mBBr-labeled protein in question is isolated individually from chloroplasts by immunoprecipitation and its thiol redox status is determined quantitatively by combining sodium dodecyl sulfate-polyacrylamide gel electrophoresis and fluorescence measurements. The findings indicate that each member of the ferredoxin/thioredoxin system containing a catalytically active thiol group is reduced in isolated intact chloroplasts after a 2-min illumination. The extents of reduction were FTR, 38%; thioredoxin m, 75% (11-kDa form) and 87% (13-kDa form); thioredoxin f, 95%. Reduction of each of these components was negligible both in the dark and when chloroplasts were transferred from light to dark conditions. The target enzyme, NADP-malate dehydrogenase, also underwent net reduction in illuminated intact chloroplasts. Fructose-1,6-bisphosphatase showed increased mBBr labeling under these conditions, but due to interfering gamma globulin proteins it was not possible to determine whether this was a result of net reduction as is known to take place in reconstituted assays. Related experiments demonstrated that mBBr, as well as N-ethylmaleimide, stabilized photoactivated NADP-malate dehydrogenase and fructose-1,6-bisphosphatase so that they remained active in the dark. By contrast, phosphoribulokinase, another thioredoxin-linked enzyme, was immediately deactivated following mBBr addition. These latter results provide new information on the relation between the regulatory and active sites of these enzymes.
Crawford
N A
NA
Division of Molecular Plant Biology, University of California, Berkeley 94720.
Droux
M
M
Kosower
N S
NS
Buchanan
B B
BB
D001426:Bacterial Proteins / Q000032:analysis*; D001643:Bridged Bicyclo Compounds; D002734:Chlorophyll / Q000032:analysis*; D054479:Chloroplast Thioredoxins; D002736:Chloroplasts / Q000201:enzymology*; D002846:Chromatography, Affinity; D003624:Darkness; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation / Q000187:drug effects; D005288:Ferredoxins / Q000032:analysis*; D005456:Fluorescent Dyes; D007506:Iron-Sulfur Proteins; D008027:Light; D045342:Light-Harvesting Protein Complexes; D010084:Oxidation-Reduction / Q000187:drug effects; D010088:Oxidoreductases / Q000032:analysis; D045322:Photosynthetic Reaction Center Complex Proteins; D010940:Plant Proteins / Q000032:analysis*; D011233:Precipitin Tests; D013438:Sulfhydryl Compounds / Q000032:analysis; D013879:Thioredoxins / Q000032:analysis* / Q000276:immunology
D001426:Bacterial Proteins; D001643:Bridged Bicyclo Compounds; D054479:Chloroplast Thioredoxins; D005288:Ferredoxins; D005456:Fluorescent Dyes; D007506:Iron-Sulfur Proteins; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D010940:Plant Proteins; D013438:Sulfhydryl Compounds; D002734:Chlorophyll; D013879:Thioredoxins; D010088:Oxidoreductases; C021588:ferredoxin-thioredoxin reductase; C028381:monobromobimane