title: Chromosomal transformation of Escherichia coli recD strains with linearized plasmids.
abstract: Wild-type Escherichia coli are resistant to genetic transformation by purified linear DNA, probably in part because of exonuclease activity. We demonstrate that E. coli containing a recD mutation could be easily transformed by linearized plasmids containing a selectable marker. The marker was transferred to the chromosome by homologous recombination, whereas plasmid markers not in the region of homology were lost.
authors.affiliation: Institute of Molecular Biology, University of Oregon, Eugene 97403.
authors.forename: C B
authors.initials: CB
authors.lastname: Russell
authors.forename: D S
authors.initials: DS
authors.lastname: Thaler
authors.forename: F W
authors.initials: FW
authors.lastname: Dahlquist
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D002633:Chemotaxis; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D043211:Exodeoxyribonuclease V; D005090:Exodeoxyribonucleases / Q000235:genetics / Q000502:physiology*; D005798:Genes, Bacterial; D009154:Mutation; D011995:Recombination, Genetic; D014170:Transformation, Genetic
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D005090:Exodeoxyribonucleases; D043211:Exodeoxyribonuclease V; C468322:exodeoxyribonuclease V, E coli