title: Discordant secretion of placental protein hormones in differentiating trophoblasts in vitro.
abstract: The regulation of trophoblast secretion of the placental proteins CG (hCG), placental lactogen (hPL), and pregnancy specific-beta-1-glycoprotein (SP-1) has not been fully elucidated. We therefore studied the secretion of hCG, hCG-beta subunit, hCG-alpha subunit, hPL, and SP-1, both in the basal state and after exposure to 8-bromo-cAMP, during the in vitro differentiation of cytotrophoblasts to syncytiotrophoblasts. Term placental tissue was enzymatically digested and cytotrophoblasts purified by Percoll density gradient centrifugation. At the time of seeding of 4-6 X 10(5) cells/ml in 35-mm flasks all of the cells were mononuclear and 48% contained hCG-alpha, but none contained hCG or hCG-beta by the avidin-biotin-peroxidase immuno-histochemical method. After 3 days in culture, hCG-alpha and hCG or hCG-beta were present in multinucleated syncytiotrophoblasts and in the mononuclear cytotrophoblasts. During the 5 days in culture, the secretion of hCG, hCG-alpha, hPL and SP-1 into the media increased and reached a maximum on day 4 followed by a decrease on day 5. Basal hCG-beta secretion was very low and did not change during culture. The ratio of hCG-alpha/hCG decreased from days 1-4 of culture. Incubation with 8-bromo-cAMP for 24 h stimulated the secretion of hCG and hCG-alpha, whereas hCG-beta and hPL levels did not change. The secretion of SP-1, however, was inhibited by 8-bromo-cAMP. These results indicate that the cytotrophoblasts secrete hCG, hCG-beta and hCG-alpha during in vitro differentiation into syncytiotrophoblasts. Since the basal ratio of hCG to hCG-alpha secretion changed during 5 days in culture and a cAMP analogue differentially modulated the secretion of the different placental protein hormones, the physiological regulation of secretion of each of the proteins also may differ.
authors.affiliation: Department of Medicine, Cedars-Sinai Medical Center-UCLA School of Medicine 90048.
authors.forename: Y
authors.initials: Y
authors.lastname: Kato
authors.forename: G D
authors.initials: GD
authors.lastname: Braunstein
mesh_terms: D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D002454:Cell Differentiation; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000378:metabolism; D003470:Culture Media / Q000032:analysis; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D010926:Placental Hormones / Q000378:metabolism*; D010928:Placental Lactogen / Q000378:metabolism; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000378:metabolism*; D011268:Pregnancy-Specific beta 1-Glycoproteins / Q000378:metabolism; D014327:Trophoblasts / Q000378:metabolism*
chemical_list: D006063:Chorionic Gonadotropin; D003470:Culture Media; D010926:Placental Hormones; D011257:Pregnancy Proteins; D011268:Pregnancy-Specific beta 1-Glycoproteins; D015124:8-Bromo Cyclic Adenosine Monophosphate; D010928:Placental Lactogen