Purification and properties of RNA polymerase from acidophilic heterotroph SJH.
RNA polymerase was isolated from the acidophilic heterotrophic bacterium SJH and purified to homogeneity. Subunit structure (beta', beta, theta, alpha 2) resembled that of other gram-negative bacteria such as Escherichia coli. pH optimum of the enzyme was around 8.0, although the bacterium grew only below pH 6.0 and optimally between 3.0-4.0. SJH and E. coli RNA polymerases showed maximal activities on homologous high molecular-weight DNA templates.
Beebee
T J
TJ
Department of Biochemistry, University of Sussex, Brighton, U.K.
D001419:Bacteria / Q000201:enzymology*; D004269:DNA, Bacterial / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D013698:Templates, Genetic
D004269:DNA, Bacterial; D012321:DNA-Directed RNA Polymerases