In vitro microbicidal activity of avian peritoneal macrophages.
Peritoneal exudate macrophages collected from 4-wk-old broilers were capable of in vitro bactericidal activity against Escherichia coli and fungistatic activity against Candida tropicalis. Bactericidal activity was inhibited by treatment of macrophages with 2-deoxyglucose. These assays may be used to evaluate macrophage function in normal, diseased, and immunomodulated birds or to assess the direct effects of cytokines and toxins on macrophage microbicidal activity.
Harmon
B G
BG
Department of Veterinary Pathology, College of Veterinary Medicine, University of Georgia, Athens 30602.
Glisson
J R
JR
D000818:Animals; D002175:Candida / Q000276:immunology*; D002645:Chickens / Q000276:immunology*; D004926:Escherichia coli / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D010529:Peritoneal Cavity / Q000166:cytology