Growth factor stimulation of adult articular cartilage.
We have examined the effect of peptide growth factors on DNA and proteoglycan synthesis by adult bovine articular cartilage in organ culture. The actions of somatomedin-C/insulin-like growth factor I (Sm-C/IGF-I), insulin, epidermal growth factor (EGF), and fibroblast growth factor (FGF) from bovine pituitary were investigated individually and in combination. FGF stimulated a 10-fold increase in tritiated thymidine incorporation while other factors used individually did not influence mitotic activity. Used in concert, insulin with EGF and insulin with FGF acted synergistically in stimulating DNA synthesis 20-fold and 40-fold, respectively. All of these growth factors, acting individually, significantly enhanced radiosulfate incorporation. This stimulation was additive for Sm-C/IGF-I in combination with EGF or FGF, but not with insulin. These data indicate that adult bovine articular chondrocytes possess the capacity to augment both mitotic and differentiated cell functions in response to growth factors. The data further suggest that, with the exception of insulin and Sm-C/IGF-I, which appear to share a common mechanism of action, these factors produce their cellular effects via different receptor or postreceptor pathways.
Osborn
K D
KD
Department of Orthopaedics, Massachusetts General Hospital, Boston 02114.
Trippel
S B
SB
Mankin
H J
HJ
D000818:Animals; D002358:Cartilage, Articular / Q000187:drug effects / Q000502:physiology*; D002417:Cattle; D004247:DNA / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005260:Female; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D008938:Mitosis / Q000187:drug effects; D009924:Organ Culture Techniques; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013268:Stimulation, Chemical; D013936:Thymidine / Q000378:metabolism
D004338:Drug Combinations; D006133:Growth Substances; D007328:Insulin; D012710:Serum Albumin, Bovine; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor; D007334:Insulin-Like Growth Factor I; D004247:DNA; D013936:Thymidine