Flow cytometry using paraffin-embedded tissue: five years on.
The use of paraffin-embedded tissue for flow cytometry is reviewed. A number of technical modifications of the original 1983 method have been described, aimed at improving the accuracy of DNA measurements by minimizing cell debris or reducing coefficients of variation, and at simplifying sample preparation. Over 100 clinical studies have now been reported, mainly assessing the effect of DNA index on prognosis, and those published up until mid-1988 are summarized in an appendix. More recently there have been developments in the use of monoclonal antibodies to measure oncogene products or proliferation markers in addition to DNA content. Detailed clinical evaluation and standardization of these more sophisticated methods is still some way ahead, but as was the case with DNA index, the use of archival material from patients whose outcome is already known should speed this process.
Hedley
D W
DW
Ludwig Institute for Cancer Research (Sydney Branch), University of Sydney, N.S.W., Australia.
D000818:Animals; D004247:DNA / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods*; D006652:Histological Techniques; D006801:Humans; D008867:Microtomy
D004247:DNA