Molecular cloning and expression of the O4 polysaccharide gene cluster from Escherichia coli.
The Escherichia coli O4 serotype is common among isolates from urinary tract infections. The genes responsible for the biosynthesis of the O4 polysaccharide in a human uropathogenic E. coli were cloned and expressed in E. coli K-12. The recombinant plasmid pGH60, which conferred the O4 phenotype, encoded eight proteins with apparent molecular weights of 39, 36.5, 35, 32.8, 26, 24, 20.7 and 13 kDa.
Haraguchi
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Department of Microbiology and Immunology, Baylor College of Medicine, Houston, Texas 77030.
Hull
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Krallmann-Wenzel
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Hull
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D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology; D003001:Cloning, Molecular; D015342:DNA Probes; D004926:Escherichia coli / Q000235:genetics* / Q000276:immunology; D005810:Multigene Family; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis / Q000235:genetics*; D015183:Restriction Mapping; D014552:Urinary Tract Infections / Q000382:microbiology
D000942:Antigens, Bacterial; D015342:DNA Probes; D011135:Polysaccharides, Bacterial