title: Molecular cloning and expression of the O4 polysaccharide gene cluster from Escherichia coli.
abstract: The Escherichia coli O4 serotype is common among isolates from urinary tract infections. The genes responsible for the biosynthesis of the O4 polysaccharide in a human uropathogenic E. coli were cloned and expressed in E. coli K-12. The recombinant plasmid pGH60, which conferred the O4 phenotype, encoded eight proteins with apparent molecular weights of 39, 36.5, 35, 32.8, 26, 24, 20.7 and 13 kDa.
authors.affiliation: Department of Microbiology and Immunology, Baylor College of Medicine, Houston, Texas 77030.
authors.forename: G E
authors.initials: GE
authors.lastname: Haraguchi
authors.forename: R A
authors.initials: RA
authors.lastname: Hull
authors.forename: U
authors.initials: U
authors.lastname: Krallmann-Wenzel
authors.forename: S I
authors.initials: SI
authors.lastname: Hull
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology; D003001:Cloning, Molecular; D015342:DNA Probes; D004926:Escherichia coli / Q000235:genetics* / Q000276:immunology; D005810:Multigene Family; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis / Q000235:genetics*; D015183:Restriction Mapping; D014552:Urinary Tract Infections / Q000382:microbiology
chemical_list: D000942:Antigens, Bacterial; D015342:DNA Probes; D011135:Polysaccharides, Bacterial