In vitro proteolytic cleavage of the Escherichia coli Ada protein by the ompT gene product.
Down regulation of the adaptive response to alkylation damage in Escherichia coli has been proposed to occur by proteolytic cleavage of the regulatory Ada protein. In this paper, it is shown that proteolysis of the Ada protein as observed in cell extracts is caused by the ompT gene product. This protease, however, was not involved in switching off the adaptive response in vivo.
Sedgwick
B
B
Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire, United Kingdom.
D001425:Bacterial Outer Membrane Proteins / Q000502:physiology*; D004926:Escherichia coli / Q000502:physiology*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D006868:Hydrolysis; D008745:Methylation; D010447:Peptide Hydrolases / Q000502:physiology*; D014157:Transcription Factors / Q000502:physiology*
D001425:Bacterial Outer Membrane Proteins; D014157:Transcription Factors; D010447:Peptide Hydrolases