Use of gene replacement to construct Escherichia coli strains carrying mutations in two genes required for stability of multicopy plasmids.
Escherichia coli mutants completely defective in ColE1 cer-mediated site-specific recombination have been mapped to two genes, xerA and xerB. In this study, xerA xerB double mutants were constructed by gene replacement with a lambda dv plasmid and were shown to be both viable and defective in ColE1 site-specific recombination.
Flinn
H
H
Institute of Genetics, University of Glasgow, United Kingdom.
Burke
M
M
Stirling
C J
CJ
Sherratt
D J
DJ
D004261:DNA Replication; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005818:Genetic Engineering / Q000379:methods; D010957:Plasmids; D011995:Recombination, Genetic