Purification and characterization of Saccharomyces cerevisiae transcription factor IIIA.
Saccharomyces cerevisiae Transcription Factor IIIA (TFIIIA) has been purified to apparent homogeneity. Two polypeptides copurified with TFIIIA activity. Yeast TFIIIA is a DNA-binding protein which exhibits a high affinity for the internal control region of the homologous 5 S ribosomal RNA gene. Characterization of the yeast protein indicates that it shares most, but not all, of the molecular properties of its Xenopus TFIIIA counterpart.
Wang
C K
CK
Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
Weil
P A
PA
D000818:Animals; D001483:Base Sequence; D004275:DNA, Ribosomal / Q000235:genetics; D004268:DNA-Binding Proteins; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012335:RNA, Ribosomal / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D035582:Transcription Factor TFIIIA; D014157:Transcription Factors / Q000235:genetics / Q000302:isolation & purification*; D014981:Xenopus
D004275:DNA, Ribosomal; D004268:DNA-Binding Proteins; D012335:RNA, Ribosomal; D012341:RNA, Ribosomal, 5S; D035582:Transcription Factor TFIIIA; D014157:Transcription Factors