Proinflammatory effects of interleukin 1 in the rat air pouch.
We have utilized the 6-day air-pouch model in rats to study the local tissue response to interleukin-1 exposure. Injection of either recombinant human interleukin 1 alpha (rIL-1 alpha) or interleukin 1 beta (rIL-1 beta) directly into preformed air pouches caused a 10- to 100-fold increase in the number of white blood cells present within the pouch. On a weight basis, rIL-1 beta was more active than rIL-1 alpha. Polymorphonuclear neutrophils (PMN) represented the majority of cells entering the pouch following either a single injection or repeated daily injections of rIL-1 alpha or rIL-1 beta. Significant increases in the number of mononuclear cells present were observed only following repeated injections. Repeated injections of rIL-1 beta, but not rIL-1 alpha, also caused the accumulation of large amounts of fluid within preformed pouches and a grossly apparent thickening of the connective-tissue lining of the pouch. Microscopic examination of stained sections of pouch lining tissue indicated a proliferation of the connective-tissue elements of the lining and deposition of large quantities of extracellular collagen within the pouch wall. These findings are entirely consistent with a role for interleukin 1 in the development and perpetuation of inflammatory reactions.
Esser
R E
RE
Department of Molecular and Cell Biology, Division of G.D.Searle and Company, Skokie, Illinois 60077.
Eyerman
M C
MC
Port
C D
CD
Anderson
W
W
D000388:Air; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007249:Inflammation / Q000139:chemically induced*; D007267:Injections; D007375:Interleukin-1 / Q000633:toxicity*; D010658:Phenylglyoxal; D051381:Rats; D011919:Rats, Inbred Strains; D011994:Recombinant Proteins / Q000633:toxicity
D007375:Interleukin-1; D011994:Recombinant Proteins; D010658:Phenylglyoxal