Chymotrypsin-catalyzed hydrolysis of chromium (III) derivatives of insulin: evidence for stabilization of the protein through interactions with metal ions.
We investigated the chymotrypsin-promoted hydrolysis of a series of chromium(III)-insulin complexes containing chelating or macrocyclic ligands. It has been shown that Cr(III) stabilizes insulin against the chymotrypsin-promoted hydrolysis of the protein. The molecular weights of Cr(III) containing peptides have been estimated to be of the order of 2,700-3,700 daltons. The Cr(III) containing peptides are richer in glutamic acid than the intact insulin and are devoid of any isoleucine. High molecular weights and the observed glutamic acid/histidine ratios in Cr(III) containing peptides have been rationalized in terms of Cr(III) being associated with insulin aggregates rather than the monomer of the protein. The chymotrypsin hydrolysis of Cr(III) insulin derivatives is influenced markedly by the nature, charge, and type of Cr(III) complex with which the protein has been reacted. Arguments have been advanced that chymotrypsin-promoted hydrolysis of insulin Cr(III) derivatives does not lead to cleavages at or near every tyrosine residue.
Govindaraju
K
K
Central Leather Research Institute, Adyar, Madras, India.
Ramasami
T
T
Ramaswamy
D
D
D002857:Chromium / Q000494:pharmacology*; D002918:Chymotrypsin / Q000378:metabolism*; D004355:Drug Stability; D006868:Hydrolysis; D007328:Insulin / Q000378:metabolism*; D010446:Peptide Fragments / Q000032:analysis; D011485:Protein Binding
D007328:Insulin; D010446:Peptide Fragments; D002857:Chromium; D002918:Chymotrypsin