title: Characterization and cloning of MwoI (GCN7GC), a new type-II restriction-modification system from Methanobacterium wolfei.
abstract: R.MwoI, a type-II restriction enzyme with the new specificity 5'-GCN7GC-3', was found in extracts of the thermophilic archaebacterium, Methanobacterium wolfei. R.MwoI cleaves duplex DNA producing fragments with 3-nt, 3'-terminal extensions, thus: GCN5/N2GC. The genes coding for the MwoI restriction and modification enzymes were cloned into Escherichia coli on the plasmid vector pBR322. The clones synthesize a low level of R.MwoI endonuclease. The plasmids display incomplete MwoI-specific modification, suggesting that the clones synthesize a low level of the M.MwoI methyltransferase, too.
authors.affiliation: New England Biolabs, Inc., Beverly, MA 01915.
authors.forename: K D
authors.initials: KD
authors.lastname: Lunnen
authors.forename: R D
authors.initials: RD
authors.lastname: Morgan
authors.forename: C J
authors.initials: CJ
authors.lastname: Timan
authors.forename: J A
authors.initials: JA
authors.lastname: Krzycki
authors.forename: J N
authors.initials: JN
authors.lastname: Reeve
authors.forename: G G
authors.initials: GG
authors.lastname: Wilson
mesh_terms: D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000302:isolation & purification; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D019605:Euryarchaeota / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D005798:Genes, Bacterial; D010957:Plasmids; D013379:Substrate Specificity
chemical_list: D004269:DNA, Bacterial; C060413:endodeoxyribonuclease MwoI; D015252:Deoxyribonucleases, Type II Site-Specific