Signal transduction via MHC class II antigens on B lymphocytes.
The role of the MHC class II antigens in the activation of resting human B lymphocytes (B-Go) was examined with respect to both early and late events in the activation process. The (Ca2+)i induced by anti-IgM was enhanced in the presence of, or following pre-incubation with, an anti-MHC class II DR antibody (D1.12). Pre-incubation with a sepharose conjugated antibody (Seph.-D1.12) augmented the proliferation of B-Go in response to a sub-optimal concentration of anti-IgM. The 2D PAGE profile of B-Go differed from that of in vivo activated B lymphocytes. The 2D PAGE profile of B-Go activated by Seph.-D1.12 was not identical to the profile of B-Go activated by either anti-IgM or PMA. These data suggest that the activation of B-Go via the class II antigens shares part of the pathway of anti-IgM induced activation but does not follow an identical pathway.
Mooney
N
N
Laboratoire d'Immunogénétique Moléculaire, Paris, France.
Hivroz
C
C
Ziai-Talebian
S
S
Grillot-Courvalin
C
C
Charron
D
D
D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000276:immunology*; D002118:Calcium / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D006681:HLA-D Antigens / Q000502:physiology*; D006801:Humans; D007075:Immunoglobulin M / Q000502:physiology; D066298:In Vitro Techniques; D008213:Lymphocyte Activation / Q000502:physiology; D015398:Signal Transduction / Q000276:immunology*; D013154:Spleen / Q000166:cytology
D000911:Antibodies, Monoclonal; D006681:HLA-D Antigens; D007075:Immunoglobulin M; D002118:Calcium