title: N-RAS mutations in T-cell acute lymphocytic leukaemia: analysis by direct sequencing detects a novel mutation.
abstract: A novel mutation of the N-RAS gene of T-ALL blast cells was detected by a direct sequencing of in vitro amplified exon-1 of the N-RAS gene. Threonine (ACA) was substituted for alanine (GCA) at codon 11. This mutation would have been overlooked by conventional probe hybridization techniques. A search for other mutations in N-RAS exon-1 in T-ALL revealed a codon 13 mutation substituting aspartic acid (GAT) for glycine (GGT) in one of 18 patients. No mutations at codon 12 were detected.
authors.affiliation: Division of Hematology/Oncology, University of California, Los Angeles 90024-1678.
authors.forename: M
authors.initials: M
authors.lastname: Bar-Eli
authors.forename: H
authors.initials: H
authors.lastname: Ahuja
authors.forename: A
authors.initials: A
authors.lastname: Foti
authors.forename: M J
authors.initials: MJ
authors.lastname: Cline
mesh_terms: D001483:Base Sequence; D003062:Codon; D005091:Exons; D011905:Genes, ras; D006801:Humans; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000235:genetics*; D009154:Mutation
chemical_list: D003062:Codon