Role of homocysteine in metR-mediated activation of the metE and metH genes in Salmonella typhimurium and Escherichia coli.
The metR-mediated activation of the Salmonella typhimurium metE and metH genes was shown to be modulated by homocysteine, an intermediate in the methionine biosynthetic pathway. Homocysteine stimulates expression of a metE-lacZ gene fusion four- to fivefold by increasing transcription from the metE promoter. In contrast, homocysteine plays an inhibitory role in the metR-mediated activation of the metH gene, decreasing expression of a metH-lacZ gene fusion threefold.
Urbanowski
M L
ML
Department of Microbiology, University of Iowa, Iowa City 52242.
Stauffer
G V
GV
D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D006710:Homocysteine / Q000502:physiology*; D008715:Methionine / Q000378:metabolism*; D008780:Methyltransferases / Q000235:genetics*; D012486:Salmonella typhimurium / Q000235:genetics*; D014158:Transcription, Genetic; D014805:Vitamin B 12 / Q000502:physiology
D006710:Homocysteine; D008715:Methionine; D008780:Methyltransferases; D014805:Vitamin B 12