title: Abortive infection of the virulent phage 9NA in a fatty acid auxotroph of Salmonella typhimurium: effect of fatty acid supplementation.
abstract: A conditional (temperature sensitive) fatty acid biosynthetic mutant (fabB2) of Salmonella typhimurium does not support the development of the virulent bacteriophage 9NA even at permissive temperature (30 degrees C). A limited amount of phage DNA synthesis takes place at this temperature. When the fatty acid composition of the host membrane is altered by growing the cells at 37 degrees C in the presence of exogenous unsaturated fatty acid, differential expression of phage genes was observed. Phage specific lysozyme is induced when the cultures are supplemented with elaidic, palmitelaidic, linoleic and linolelaidic acids but not with oleic and plamitoleic acids. However, in no case were infective particles produced. Under conditions where no lysozyme is synthesized the infected cells increase in length and become filamentous.
authors.affiliation: Molecular Biology Unit, Banaras Hindu University, Varanasi, India.
authors.forename: R
authors.initials: R
authors.lastname: Goyal
authors.forename: M
authors.initials: M
authors.lastname: Chakravorty
mesh_terms: D001435:Bacteriophages / Q000254:growth & development* / Q000472:pathogenicity; D004279:DNA, Viral / Q000096:biosynthesis; D005227:Fatty Acids / Q000502:physiology*; D008563:Membrane Lipids / Q000502:physiology; D009113:Muramidase / Q000096:biosynthesis; D009154:Mutation; D019301:Oleic Acid; D009829:Oleic Acids / Q000502:physiology; D012486:Salmonella typhimurium / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D004279:DNA, Viral; D005227:Fatty Acids; D008563:Membrane Lipids; D009829:Oleic Acids; D019301:Oleic Acid; D009113:Muramidase