title: Role of glycosylation in the incorporation of intrinsic mannoproteins into cell walls of Saccharomyces cerevisiae.
abstract: Cell wall mannoproteins from Saccharomyces cerevisiae are completely or partially incorporated into their final location when N-glycosylation is inhibited by tunicamycin. These include a 90-100 kDa species still containing O-linked oligomannose chains, derived from a N-glycosylated material larger than 120 kDa; and a 30.5 kDa peptide lacking mannose residues, derived from a 33 kDa species. For both species, the growth temperature influences the level of incorporation of the non N-glycosylated molecules. Secretion of the peptides lacking N-linked saccharide chains follows the route defined by sec mutants.
authors.affiliation: Departament de Microbiologia, Facultat de Farmacia, Universitat de Valencia, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Sanz
authors.forename: E
authors.initials: E
authors.lastname: Herrero
authors.forename: R
authors.initials: R
authors.lastname: Sentandreu
mesh_terms: D002473:Cell Wall / Q000378:metabolism; D006031:Glycosylation; D008562:Membrane Glycoproteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*; D014415:Tunicamycin / Q000494:pharmacology
chemical_list: D008562:Membrane Glycoproteins; C025363:mannoproteins; D014415:Tunicamycin