Axial diffusion of retinol in isolated frog rod outer segments following substantial bleaches of visual pigment.
Partial bleaches of rhodopsin were made in either the proximal or distal halves of isolated Rana pipiens rod outer segments. The fluorescence of all-trans retinol was recorded 15, 60 and 120 min following 17% and 63% bleaches. Some of the retinol that formed remained immobilized in the bleached halves of the outer segments, while another portion was slowly mobilized and diffused along the cell axis.
Sears
R C
RC
R. S. Dow Neurological Sciences Institute, Good Samaritan Hospital and Medical Center, Portland, OR 97209-1595.
Kaplan
M W
MW
D000818:Animals; D002138:Calibration; D004058:Diffusion; D005453:Fluorescence; D008027:Light; D010786:Photoreceptor Cells / Q000378:metabolism*; D011894:Rana pipiens; D012168:Retinal Pigments / Q000378:metabolism* / Q000502:physiology; D012243:Rhodopsin / Q000378:metabolism; D012374:Rod Cell Outer Segment / Q000378:metabolism*; D014801:Vitamin A / Q000378:metabolism*
D012168:Retinal Pigments; D014801:Vitamin A; D012243:Rhodopsin