Immunohistologic characterization of epitheloid cells in Crohn's granulomas.
Monoclonal antibodies reactive with monocyte/macrophages were used to characterize the nature of epitheloid cells in Crohn's granulomas. Six micron continuous frozen sections were studied in 12 specimens. A series of monoclonal antibodies was used to stain sections by the avidin-biotin complex method. Epitheloid cells in Crohn's granulomas reacted with anti-25F9 and anti-HLA-DR antibodies, but did not respond to anti-Leu M1, anti-Leu M2, anti-Leu M3 nor anti-Leu M5 antibodies. These results suggest that epitheloid cells in Crohn's granulomas may be activated macrophages.
Miura
M
M
Third Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
Hiwatashi
N
N
Yamashita
K
K
Kimura
M
M
D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D003424:Crohn Disease / Q000276:immunology*; D004848:Epithelium / Q000276:immunology; D006099:Granuloma / Q000276:immunology*; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D008264:Macrophages / Q000276:immunology; D009000:Monocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D015214:Antigens, Differentiation, Myelomonocytic; D006684:HLA-DR Antigens