Crystallization and preliminary X-ray studies of recombinant human granulocyte-macrophage colony stimulating factor.
Human granulocyte-macrophage colony stimulating factor (hGM-CSF) is an important regulator of growth and differentiation for mononuclear and polymorphonuclear phagocytes. Here we report the crystallization and preliminary X-ray studies of Escherichia coli-expressed hGM-CSF. The crystals are orthorhombic, with the space group P212121, and have unit cell dimensions a = 46.62 A, b = 58.73 A and c = 126.42 A. Recombinant hGM-CSF crystals diffract X-rays to 2.4 A resolution and are thus suitable for X-ray structural studies.
La Londe
J M
JM
Chemistry Department, University of Pennsylvania, Philadelphia 19104.
Hanna
L S
LS
Rattoballi
R
R
Berman
H M
HM
Voet
D
D
D003115:Colony-Stimulating Factors; D003460:Crystallization; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances; D006801:Humans; D011994:Recombinant Proteins; D014961:X-Ray Diffraction
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor