title: Monoclonal antibodies in detection of choroidal melanoma.
abstract: Three monoclonal antibodies (MoAbs) prepared against cutaneous melanomas were tested against one group of 12 choroidal melanomas with indirect immunofluorescence in frozen sections. A fourth MoAb was tested in paraffin sections of a second group of 47 choroidal melanomas. One MoAb (NKI-M7) did not react with choroidal melanoma, even though it had a high sensitivity for cutaneous melanoma. A second MoAb (NKI-M6) showed a positive reaction with only 2/12 choroidal melanomas. The third MoAb (NKI/beteb) reacted with all choroidal melanomas, regardless of the cell type. MoAb NKI/C-3 was positive with 38/47 (81%) choroidal melanomas. We conclude that NKI/C-3 and NKI/beteb have a high sensitivity for both cutaneous and choroidal melanomas in frozen sections. Of these two antibodies NKI/beteb was the most specific for cutaneous naevi and melanomas.
authors.affiliation: Department of Ophthalmology, Erasmus University Rotterdam, Eye Hospital, The Netherlands.
authors.forename: P J
authors.initials: PJ
authors.lastname: Ringens
authors.forename: R
authors.initials: R
authors.lastname: van Haperen
authors.forename: C
authors.initials: C
authors.lastname: Vennegoor
authors.forename: P T
authors.initials: PT
authors.lastname: de Jong
authors.forename: S G
authors.initials: SG
authors.lastname: van Duinen
authors.forename: D J
authors.initials: DJ
authors.lastname: Ruiter
authors.forename: A W
authors.initials: AW
authors.lastname: van der Kamp
mesh_terms: D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001706:Biopsy; D002830:Choroid Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D008545:Melanoma / Q000175:diagnosis* / Q000378:metabolism; D012878:Skin Neoplasms / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal