Distribution of angiotensin II receptors and renin in the mouse fetus.
To investigate the ontogeny of the renin-angiotensin system we studied the characteristics and location of angiotensin II (AII) receptors in mouse fetuses and examined sites of renin mRNA expression by in situ hybridization and Northern blot analysis. Autoradiographic analysis of the binding of 125I-[Sar1,Ala8]AII to slide-mounted frozen sections of 17-day-old DBA/2N mice revealed abundant AII receptors widely distributed throughout the body. High receptor density was found in primitive mesenchymal tissue under the epidermis and surrounding muscle and cartilage, in skeletal and smooth muscle, and in all layers of the adrenal cortex. Lower receptor density was seen in the kidney, liver, and lungs. The autoradiographic staining was abolished by incubation of the sections with excess unlabeled AII. Scatchard analysis of the binding of 125I-[Sar1,Ala8,]AII to membrane-rich fractions of eviscerated fetuses showed a single type of high affinity receptors with a Kd of 2.9 x 10(-9) M and a receptor concentration of 3300 fmol/mg protein. Localization of renin mRNA was analyzed by in situ hybridization using an antisense 35S-labeled riboprobe transcribed from a mouse renin2 cDNA clone. Hybridization to fetal tissue sections showed high intensity staining in the kidney and adrenal cortex. Northern blot analysis confirmed the high expression of renin mRNA in the fetal kidney. The presence of an active renin-angiotensin system in the fetus was confirmed by the demonstration of renin-like activity and bioactive AII in fetal extracts. The widespread distribution of AII receptors in the fetus, compared to the discrete localization to specialized tissues in the adult, may indicate a unique role for the peptide during development.
Zemel
S
S
National Institute of Child Health and Human Development, Endocrinology and Reproduction Research Branch, Bethesda, Maryland 20892.
Millan
M A
MA
Aguilera
G
G
D000804:Angiotensin II / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001345:Autoradiography; D005260:Female; D005333:Fetus / Q000032:analysis* / Q000378:metabolism / Q000648:ultrastructure; D005786:Gene Expression Regulation; D051379:Mice; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D011945:Receptors, Angiotensin / Q000032:analysis* / Q000378:metabolism / Q000502:physiology; D012083:Renin / Q000032:analysis* / Q000235:genetics / Q000378:metabolism
D012333:RNA, Messenger; D011945:Receptors, Angiotensin; D000804:Angiotensin II; D012083:Renin