title: Nucleotide sequence of a citrate utilization gene from Citrobacter amalonaticus.
abstract: The chromosomal DNA fragment (BamHI-BglII fragment of 2,972 base pairs) conferring citrate utilization in Citrobacter amalonaticus ATCC 25405 was cloned and sequenced. Two genes (citA and citB) identified in the Cit+ determinant were found in a BamHI-BglII fragment from C. amalonaticus. Southern DNA-DNA hybridization experiments and the construction of Cit- mutants of C. amalonaticus showed that C. amalonaticus has a single copy of the cit gene on the chromosome.
authors.affiliation: Department of Veterinary Public Health, Obihiro University of Agriculture and Veterinary Medicine, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Daimon
authors.forename: N
authors.initials: N
authors.lastname: Ishiguro
authors.forename: M
authors.initials: M
authors.lastname: Shinagawa
authors.forename: G
authors.initials: G
authors.lastname: Sato
mesh_terms: D001483:Base Sequence; D015139:Blotting, Southern; D002951:Citrates / Q000378:metabolism*; D002954:Citrobacter / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009154:Mutation; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping
chemical_list: D002951:Citrates