A ras-related gene with transformation suppressor activity.
A 1.8 kb cDNA clone, Krev-1, with revertant-inducing activity on Kirsten sarcoma virus-transformed NIH/3T3 cells, has been isolated from a human fibroblast cDNA expression library. In Krev-1 transfectants, there is a correlation between the levels of specific mRNA and the degrees of suppression of the transformed phenotype. The cDNA encodes a protein of 21,000 daltons that unexpectedly shares around 50% amino acid identities with ras proteins. The Krev-1 homologs are found in mouse, rat, and chicken DNA, and their transcripts are ubiquitously expressed in many rat organs. Thus, the Krev-1 gene seems to play an important role(s) in a wide variety of tissues, and may be involved in the negative growth regulation of certain cell types.
Kitayama
H
H
Laboratory of Molecular Oncology, Tsukuba Life Science Center, Ibaraki, Japan.
Sugimoto
Y
Y
Matsuzaki
T
T
Ikawa
Y
Y
Noda
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002472:Cell Transformation, Viral; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D011905:Genes, ras; D006801:Humans; D005810:Multigene Family; D051381:Rats
D004247:DNA