The development of an immunosensor for the electrochemical determination of the isoenzyme LDH5.
In this work we describe an immunoelectrochemical technique for the determination of LDH isoenzymes. The method is based on the covalent binding of monoclonal antibody against the isoenzyme LDH5 to a glassy carbon electrode, and the exploitation of the electrode surface as the solid phase for enzyme immunoassay. The resulting enzyme electrode was studied by a computerized electrochemical system. This combination formed the basis of a highly sensitive fast and convenient technique for the determination of the LDH isoenzymes in sera.
Rishpon
J
J
Department of Biotechnology, Faculty of Life science, Tel-Aviv University, Ramat Aviv, Israel.
Rosen
I
I
D000818:Animals; D000911:Antibodies, Monoclonal; D015374:Biosensing Techniques; D004563:Electrochemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000032:analysis* / Q000097:blood / Q000276:immunology
D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase