The effect of ASP on the adipocyte of the morbidly obese.
The control of triglyceride synthesis within the adipocyte is not fully understood. Insulin is considered to be the most potent stimulant of triglyceride synthesis. In this paper, we report on the effect of a small (14000 Da), basic (pI 9.0) protein isolated from human serum. This protein has been called acylation stimulating protein (ASP). It is a potent stimulant of triglyceride synthesis in adipocytes from both normal weight and morbidly obese subjects. Its stimulatory effect on adipocytes is both rapid, occurring between 15-30 min after the start of incubation, and prolonged, lasting for up to 3 hr. Compared to insulin, it is sixfold more potent in its effect on triglyceride synthesis. As well as acting on isolated cells, ASP also has a fourfold stimulatory effect on triglyceride synthesis in human adipose microsomes at a concentration of 25 micrograms/ml. This study indicates that ASP is a potent stimulant of triglyceride synthesis and therefore may play a role in the pathogenesis of morbid obesity.
Walsh
M J
MJ
Royal Victoria Hospital, McGill University, Montreal, Quebec, Canada.
Sniderman
A D
AD
Cianflone
K
K
Vu
H
H
Rodriguez
M A
MA
Forse
R A
RA
D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D001798:Blood Proteins / Q000494:pharmacology*; D015926:Complement C3a / Q000031:analogs & derivatives*; D004075:Diglycerides / Q000096:biosynthesis*; D005260:Female; D005989:Glycerides / Q000096:biosynthesis*; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D008297:Male; D009767:Obesity, Morbid / Q000378:metabolism*; D013997:Time Factors; D014280:Triglycerides / Q000096:biosynthesis*
D001798:Blood Proteins; D004075:Diglycerides; D005989:Glycerides; D007328:Insulin; D014280:Triglycerides; C044541:complement C3a, des-Arg-(77)-; D015926:Complement C3a