Influence of ethanol on fetal brain cholinergic enzyme activities.
Cultured brain cells from rat fetuses of ethanol-treated mothers demonstrated more than 2-fold elevations in choline acetyltransferase (ChAT) activity relative to those of control (saline-exposed) fetal brain cells. When cells from control animals were incubated in vitro for 5 days with 0.1% ethanol, ChAT activity was found to increase more than 4-fold. Brain cells from in utero ethanol-treated animals further exposed to ethanol in vitro for 5 days demonstrated significantly higher ChAT activity compared to cells exposed to ethanol only in vivo. These levels were more than 6 times greater than those of central nervous system cells never exposed to ethanol. Acetylcholinesterase (AChE) activity was significantly elevated (greater than 4-fold) in fetal brain cells when ethanol was present both in vivo and in vitro, but neither treatment alone resulted in any significant changes in AChE. These effects of ethanol on enzymes involved in acetylcholine metabolism may contribute to the different developmental neurologic abnormalities associated with fetal alcohol exposure.
Okonmah
A D
AD
College of Pharmacy, Florida A. & M. University, Tallahassee.
Brown
J W
JW
Fishman
L M
LM
Carballeira
A
A
Soliman
K F
KF
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000201:enzymology* / Q000473:pathology; D002478:Cells, Cultured; D002795:Choline O-Acetyltransferase / Q000378:metabolism*; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D005333:Fetus / Q000201:enzymology*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D011919:Rats, Inbred Strains
D000431:Ethanol; D002795:Choline O-Acetyltransferase; D000110:Acetylcholinesterase