Structural studies on phosphorylated oligosaccharides derived from yeast mannan by 1H(31P) relayed spin-echo difference spectroscopy (RESED).
Phosphorylated and nonphosphorylated tri- and tetrasaccharides were isolated from yeast mannan and analyzed by 500-MHz 1H NMR spectroscopy. Relayed spin-echo difference spectroscopy (RESED) was used to reveal subspectra of the phosphorylated residues. In this way the attachment position of the phosphate group could be established. The structures of the phosphorylated compounds turned out to be (Formula: see text) and (Formula: see text) RESED spectroscopy has proved to be a suitable method in analyzing low amounts of phosphorylated oligosaccharides.
de Waard
P
P
Department of Bio-organic Chemistry, Utrecht University, The Netherlands.
Vliegenthart
J F
JF
Kozutsumi
Y
Y
Kawasaki
T
T
Yamashina
I
I
D000469:Alkaline Phosphatase; D000818:Animals; D002236:Carbohydrate Conformation; D006859:Hydrogen; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008322:Mammals; D008360:Mannosephosphates / Q000032:analysis; D009844:Oligosaccharides / Q000302:isolation & purification; D010758:Phosphorus; D010766:Phosphorylation; D012441:Saccharomyces cerevisiae / Q000032:analysis*
D008360:Mannosephosphates; D009844:Oligosaccharides; D010758:Phosphorus; D006859:Hydrogen; D000469:Alkaline Phosphatase