title: Effect of red cell hypertransfusion on thrombocytopoietic regeneration following irradiation and bone marrow transplantation in the mouse.
abstract: To examine the influence of suppressed erythropoiesis on platelet recovery following marrow injury, C57B1/6 mice were given red cell transfusion or Ringer's citrate. Twenty-four hours later, the animals were given either lethal (9 Gy) irradiation followed by syngeneic marrow or sublethal (3.5 Gy) irradiation and allowed to recover. The adequacy of erythroid suppression was shown by a marked decline in erythroid colony-forming cells (CFU-E) in both marrow and spleen. In both irradiation groups, platelet counts were lower in the hypertransfused mice when compared to nontransfused controls. This decrease in platelet count was observed irrespective of the degree of polycythemia or the time of observation. To determine whether red cell hypertransfusion also affects megakaryocytopoiesis, megakaryocyte colony-forming cells (CFU-MK), megakaryocyte numbers in femoral marrow sections, and megakaryocyte volumes were measured. An increase in CFU-MK-derived colonies was observed in the marrows of hypertransfused animals in both irradiation groups, and in the spleen in the group which received 3.5 Gy. Megakaryocyte numbers and volumes in femoral marrow sections were increased in the hypertransfused mice given 3.5 Gy, but not in the group given 9 Gy. The data show that despite complex effects on megakaryocytes and their progenitors, red cell hypertransfusion delays rather than enhances platelet recovery.
authors.affiliation: Department of Basic and Clinical Research, Scripps Clinic, La Jolla, CA 92037.
authors.forename: C
authors.initials: C
authors.lastname: Chatelain
authors.forename: S A
authors.initials: SA
authors.lastname: Burstein
authors.forename: S K
authors.initials: SK
authors.lastname: Erb
authors.forename: J W
authors.initials: JW
authors.lastname: Adamson
authors.forename: L A
authors.initials: LA
authors.lastname: Harker
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000502:physiology* / Q000528:radiation effects; D001803:Blood Transfusion / Q000379:methods; D016026:Bone Marrow Transplantation; D004307:Dose-Response Relationship, Radiation; D017707:Erythrocyte Transfusion; D004920:Erythropoiesis / Q000528:radiation effects; D006410:Hematopoiesis / Q000528:radiation effects*; D008297:Male; D008533:Megakaryocytes / Q000502:physiology / Q000528:radiation effects; D051379:Mice; D008810:Mice, Inbred C57BL; D011086:Polycythemia / Q000097:blood; D011828:Radiation Chimera