Detection of dengue 4 virus core protein in the nucleus. II. Antibody against dengue 4 core protein produced by a recombinant baculovirus reacts with the antigen in the nucleus.
The dengue 4 virus (DEN-4) core gene and part of the PreM genes were inserted into the baculovirus polyhedrin gene region. The recombinant baculovirus directed the synthesis of the DEN-4 core protein fused to a part of the polyhedrin protein (Mr 25K), as determined by Western blot analysis using DEN-4 core monoclonal antibody. A mouse polyclonal antibody prepared against the DEN-4 core fusion protein showed antigenic reactivity with the authentic DEN-4 core protein (Mr 15.5K) present in the nucleus as well as in the cytoplasm of DEN-4-infected Vero cells as demonstrated by the peroxidase-antiperoxidase staining method. This antibody did not react with cells infected with DEN-1, -2, -3 or Japanese encephalitis virus, or mock-infected cells.
Makino
Y
Y
Department of Virology, School of Medicine, University of the Ryukyus, Okinawa, Japan.
Tadano
M
M
Anzai
T
T
Ma
S P
SP
Yasuda
S
S
Fukunaga
T
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D015153:Blotting, Western; D002467:Cell Nucleus / Q000276:immunology*; D002478:Cells, Cultured; D003715:Dengue / Q000276:immunology; D003716:Dengue Virus / Q000235:genetics / Q000276:immunology*; D005814:Genes, Viral; D007124:Immunoenzyme Techniques; D007304:Insect Viruses / Q000235:genetics / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000235:genetics / Q000276:immunology; D011995:Recombination, Genetic; D014758:Viral Core Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D011993:Recombinant Fusion Proteins; D014758:Viral Core Proteins