Quantitation and immunohistochemical localization of cathepsins E and D in rat tissues and blood cells.
The distribution of cathepsins E and D in various rat tissues and blood cells was determined by immunoprecipitation and by immunohistochemistry with discriminative antibodies specific for each enzyme. While cathepsin D was detected in all of the tissues and blood cells tested (except for erythrocytes), cathepsin E had a relatively limited distribution. The cathepsin E content was highest in the stomach and was succeeded in the following order by the urinary bladder, thymus, spleen, cervical lymph node and bone marrow. Significant amounts of cathepsin E were also found in the colon, rectum, jejunum, skin, lung, kidney and submandibular gland. The other tissues tested had little or no detectable cathepsin E content. Of the blood cells tested, lymphocytes and peritoneal neutrophils contained high levels of cathepsin E. Erythrocytes had cathepsin E only as aspartic proteinases. When the subcellular localization of cathepsin E in the neutrophils was investigated by fractionation of the postnuclear supernatants, the enzyme behaved as a soluble cytosolic enzyme. In contrast, cathepsin D was mainly associated with the granular fraction. The immunohistochemical localization of cathepsins E and D was clearly different in the stomach, large intestines, kidney and urinary bladder, but was similar in the lymph node and spleen. The tissue-fixed macrophages, which were notable in the skeletal and cardiac muscle tissues, submucosal layers of the gastrointestinal tracts, salivary gland, lung and trachea, also exhibited similar intense immunoreactivities demonstrative of both cathepsins E and D.
Sakai
H
H
Department of Pharmacology, Nagasaki University School of Dentistry, Japan.
Saku
T
T
Kato
Y
Y
Yamamoto
K
K
D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000906:Antibodies; D002402:Cathepsin D / Q000097:blood / Q000378:metabolism*; D020059:Cathepsin E; D002403:Cathepsins / Q000097:blood / Q000378:metabolism*; D004064:Digestive System / Q000201:enzymology; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008221:Lymphoid Tissue / Q000201:enzymology; D009504:Neutrophils / Q000201:enzymology; D009928:Organ Specificity; D051381:Rats; D013347:Subcellular Fractions / Q000201:enzymology
D000906:Antibodies; D007770:L-Lactate Dehydrogenase; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D002403:Cathepsins; D020059:Cathepsin E; D002402:Cathepsin D