Chloroplast and nuclear genomes of Chlamydomonas reinhardtii share homology with Escherichia coli genes for DNA replication, repair and transcription.
Most of the cpDNA genes studied to date are genes encoding elements of the photosynthetic apparatus and translational machinery. Much less is known about genes encoding the polypeptides involved in transcription, cpDNA replication, recombination and repair. The similarities between bacterial and some cpDNA genes were exploited to identify some of these chloroplast genes using bacterial probes. Probes derived from the Escherichia coli genes dnaA, recA, uvrC, transcriptional factor rho, and rpoC were used to search for homologous DNA sequences in chloroplast and nuclear genomes of Chlamydomonas reinhardtii. Regions homologous to all of these genes were located on the cpDNA physical map by probing restriction fragments of cpDNA with plasmid fragments containing these genes. Probing nuclear DNA with bacterial gene probes revealed DNA fragments homologous to dnaA and rpoC genes.
Oppermann
T
T
Department of Biology, Indiana University, Bloomington 47405.
Hong
T H
TH
Surzycki
S J
SJ
D015139:Blotting, Southern; D002467:Cell Nucleus; D002696:Chlamydomonas / Q000235:genetics*; D002736:Chloroplasts; D015342:DNA Probes; D004260:DNA Repair; D004261:DNA Replication; D004269:DNA, Bacterial / Q000235:genetics; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D004926:Escherichia coli / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D009876:Operon; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D015342:DNA Probes; D004269:DNA, Bacterial; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI