Immunohistochemical detection of biotin-labeled murine monoclonal antibody in nude mice transplanted with human colon cancer.
The tissue localization of biotin-labeled murine monoclonal antibody (MAb) A7 directed against human colonic cancer was examined in tumor-bearing nude mice following intravenous administration. MAb A7 did not react with any murine visceral organs. At 24 hr after biotin-labeled A7 injection, the tumor was stained by the avidin-biotin peroxidase complex method, but no staining was observed after injection of biotin-labeled normal mouse Ig G in the control group. Biotin-labeled MAb was found to be suitable for the detection of the xenografted tumor of nude mice. Our purpose was to provide a new method concerning the distribution of biotin-labeled MAb in vivo.
Yokota
T
T
First Department of Surgery, Kyoto Prefectural University of Medicine, Japan.
Takahashi
T
T
Yamaguchi
T
T
Kitamura
K
K
Sawai
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D001710:Biotin / Q000032:analysis*; D002460:Cell Line; D003110:Colonic Neoplasms / Q000032:analysis*; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014183:Transplantation, Heterologous
D000911:Antibodies, Monoclonal; D001710:Biotin