Large-scale purification of human islets utilizing discontinuous albumin gradient on IBM 2991 cell separator.
A new method is described for the large-scale purification of human pancreatic islets with a discontinuous gradient of bovine serum albumin formed on an IBM 2991 cell separator. Fifteen human pancreases were processed, and after density-gradient centrifugation, a mean of 2643 islets/ml pancreatic digest were recovered with a mean purity of 63% and contained in 430 microliter mean vol. Viability of gradient-isolated islets was compared with that of non-density-gradient islets (handpicked) and showed no difference in function. This technique allows isolation of intact, viable human islets of Langerhans of sufficient purity for potential human transplantation.
Lake
S P
SP
Department of Surgery, University of Leicester, England, United Kingdom.
Bassett
P D
PD
Larkins
A
A
Revell
J
J
Walczak
K
K
Chamberlain
J
J
Rumford
G M
GM
London
N J
NJ
Veitch
P S
PS
Bell
P R
PR
D000328:Adult; D000818:Animals; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000601:surgery; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology*; D051381:Rats; D011923:Rats, Nude