AIDS-Kaposi's sarcoma-derived cells express cytokines with autocrine and paracrine growth effects.
When grown in vitro, cells from Kaposi's sarcoma lesions of AIDS patients (AIDS-KS cells) constitutively release several growth promoting activities. When inoculated into nude mice, the AIDS-KS cells induce a KS-like lesion of mouse origin. Here it is shown that the AIDS-KS cells express messenger RNA for a complex mixture of cytokines that correlate with several of the biological activities of these cells. Basic fibroblast growth factor, which is a potent angiogenic factor, and interleukin-1 messenger RNAs are expressed at very high levels and seem to account for a large proportion of the activities, since their corresponding proteins are released in biologically active form into the culture media where they induce autocrine and paracrine growth effects.
Ensoli
B
B
Laboratory of Tumor Cell Biology, National Cancer Institute, Bethesda, MD 20892.
Nakamura
S
S
Salahuddin
S Z
SZ
Biberfeld
P
P
Larsson
L
L
Beaver
B
B
Wong-Staal
F
F
Gallo
R C
RC
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications*; D001685:Biological Factors / Q000235:genetics*; D016207:Cytokines; D005346:Fibroblast Growth Factors / Q000235:genetics; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D012016:Reference Values; D012514:Sarcoma, Kaposi / Q000209:etiology / Q000235:genetics* / Q000473:pathology; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured / Q000166:cytology*
D001685:Biological Factors; D016207:Cytokines; D007375:Interleukin-1; D012333:RNA, Messenger; D005346:Fibroblast Growth Factors