Production of IgE antibodies to mite in guinea pigs by nasal immunization.
There has been no report that IgE antibodies can be raised in guinea pigs by immunizations through respiratory tracts. We dropped a 0.1 ml mixture of 50 micrograms mite extract and aluminium hydroxide gel (Alum) into each of both nostrils of 5 guinea pigs. A mite extract alone was dropped into the nostrils of another 5 guinea pigs. The immunization was done every week. IgE antibodies were detected in one of guinea pigs sensitized 5 times with Alum + mite and also in another guinea pig in the same group sensitized 10 times. No IgE antibodies were detected in the animals immunized with mite alone.
Ishii
A
A
Ito
K
K
Ino
Y
Y
Murakami
S
S
Miyamoto
T
T
D000534:Alum Compounds / Q000008:administration & dosage; D000818:Animals; D000941:Antigens / Q000008:administration & dosage / Q000276:immunology; D006168:Guinea Pigs; D007114:Immunization / Q000379:methods; D007073:Immunoglobulin E / Q000096:biosynthesis*; D008297:Male; D008925:Mites / Q000276:immunology*; D009666:Nose
D000534:Alum Compounds; D000941:Antigens; C041524:aluminum sulfate; D007073:Immunoglobulin E