Evaluation of the EMIT amitriptyline and nortriptyline assays for the determination of serum clomipramine and desmethylclomipramine.
Homogeneous enzyme immunoassay reagents (EMIT) developed for the measurement of amitriptyline and nortriptyline in serum were modified to allow quantitation of clomipramine and desmethylclomipramine. The method was compared to a high-performance liquid chromatographic method. Between-run precision [coefficient of variation (CV)] for clomipramine in the EMIT assay for amitriptyline ranged from 2.6 to 3.2%. For desmethylclomipramine in the nortriptyline assay, the between-run CV ranged from 1.4 to 1.9%. Serum specimens from 43 patients (desmethylclomipramine) and 59 patients (clomipramine) were analyzed by both methods, with good correlation between methods. For clomipramine, recovery ranged from 100 to 102% (0-600 ng/ml range) and was 95-103% for desmethylclomipramine (0-600 ng/ml). The modified EMIT assays offered sufficient reproducibility, accuracy, and correlation with an established method for routine analysis of clomipramine and desmethylclomipramine.
Fraser
A D
AD
Toxicology Laboratory, Victoria General Hospital, Halifax, Nova Scotia, Canada.
Bryan
W
W
Isner
A F
AF
D000639:Amitriptyline / Q000097:blood*; D000918:Antibody Specificity; D002997:Clomipramine / Q000031:analogs & derivatives* / Q000097:blood*; D003429:Cross Reactions; D005069:Evaluation Studies as Topic; D006801:Humans; D007124:Immunoenzyme Techniques; D007202:Indicators and Reagents; D009661:Nortriptyline / Q000097:blood*
D007202:Indicators and Reagents; C012273:desmethylclomipramine; D000639:Amitriptyline; D009661:Nortriptyline; D002997:Clomipramine