[Recording of the respiration of skinned cardiac fibers--a method that permits an evaluation of the functional parameters of mitochondria without their isolation from the tissue].
Respiratory parameters of cardiac mitochondria were determined in the bundles of cardiac fibers skinned by saponin that specifically removed sarcolemma but left intracellular structures intact. The described method can be used for determination of the mitochondrial respiratory parameters in small biopsy samples from the human heart.
Kuznetsov
A V
AV
Veksler
V I
VI
Sharov
V G
VG
Kapel'ko
V I
VI
Saks
V A
VA
D000818:Animals; D003327:Coronary Disease / Q000378:metabolism / Q000473:pathology; D003584:Cytological Techniques; D007545:Isoproterenol / Q000494:pharmacology; D008854:Microscopy, Electron / Q000379:methods; D008929:Mitochondria, Heart / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D012503:Saponins / Q000494:pharmacology; D012996:Solutions
D012503:Saponins; D012996:Solutions; D007545:Isoproterenol
Registratsiia dykhaniia skinirovannykh volokon serdtsa--metod, pozvoliaiushchiĭ otsenivat' funktsional'nye parametry mitokhondriĭ bez ikh vydeleniia iz tkani.