B lymphocyte derived hematopoietic growth factors.
The present study was undertaken to elucidate the effect of bacterial lipopolysaccharides (LPS) on B lymphocyte derived hematopoietic growth factors. Bone marrow-derived (BM) B cells and homogeneous B cell lines (lymphomas and hybridomas) were used to study the production of granulocyte-macrophage colony stimulating factor (GM-CSF). The BM B cells required two signals for GM-CSF production: LPS and a tumor promoting phorbol ester (TPA). In the B cell lines, GM-CSF production was inducible with LPS alone. Molecular analysis of the GM-CSF gene expression revealed that steady state levels (as determined by Northern analysis) of GM-CSF mRNA could be detected only in LPS-induced cells, whereas it was found by nuclear transcription analysis that the GM-CSF gene is transcribed even in uninduced cells. However, biological activity of GM-CSF was detected only in supernatants from LPS-induced cells. These data suggest a post-transcriptional regulation of this gene.
Pluznik
D H
DH
Laboratory of Immunology, National Institute of Dental Research, Bethesda, MD 20892.
Bickel
M
M
Mergenhagen
S E
SE
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D002460:Cell Line; D003115:Colony-Stimulating Factors / Q000096:biosynthesis* / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000096:biosynthesis* / Q000235:genetics; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D008070:Lipopolysaccharides; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D013755:Tetradecanoylphorbol Acetate