Rapid methods to visualize Y-specific repeated DNA sequences in cytological preparations.
We described rapid methods to detect Y-specific repeated DNA sequences in cytological preparations using in situ hybridization. A human Y chromosome specific DNA probe with an insert equivalent to that in pHY2.1 was labelled with [alpha-32P]dCTP or photobiotin, and hybridized to chromosome preparations. Signals were visualized specifically on Y chromosomes after 1 day's autoradiography or a couple of hours treatment with streptavidin alkaline phosphatase/BCIP/NBT. These methods are useful for molecular confirmation of Y-autosomal translocations.
Matsubara
T
T
Ikeda
S
S
Narahara
K
K
Kimoto
H
H
Oda
T
T
D004247:DNA / Q000235:genetics; D015342:DNA Probes; D005821:Genetic Techniques; D006801:Humans; D008297:Male; D012091:Repetitive Sequences, Nucleic Acid; D014178:Translocation, Genetic; D014998:Y Chromosome
D015342:DNA Probes; D004247:DNA