Determination of aspartame in beverages using an alcohol oxidase enzyme electrode.
A new method for the determination of the artificial sweetener aspartame is described. alpha-Chymotrypsin is used to cleave the methyl ester group of aspartame, producing methanol hydrolytically. The methanol is detected using an electrode which is constructed by physically trapping yeast alcohol oxidase enzyme at the tip of a dissolved oxygen electrode. The decrease in oxygen concentration, which occurs as methanol is enzymatically oxidized to formaldehyde, is measured amperometrically. Aspartame levels in diet soft drinks as determined by the proposed method and by liquid chromatography are in excellent agreement. The relative standard deviation of the measurements is 0.83%. The methanol present in diet cola as a result of aspartame degradation can also be measured by using the electrode without alpha-chymotrypsin.
Smith
V J
VJ
Phillips Petroleum Co., Biotechnology Division, Bartlesville, OK 74004.
Green
R A
RA
Hopkins
T R
TR
D000429:Alcohol Oxidoreductases; D001218:Aspartame / Q000032:analysis*; D001628:Beverages / Q000032:analysis*; D015374:Biosensing Techniques; D002853:Chromatography, Liquid; D002918:Chymotrypsin; D004032:Diet; D004151:Dipeptides / Q000032:analysis*; D006868:Hydrolysis; D007202:Indicators and Reagents; D000432:Methanol / Q000032:analysis
D004151:Dipeptides; D007202:Indicators and Reagents; D000429:Alcohol Oxidoreductases; D002918:Chymotrypsin; D000432:Methanol; D001218:Aspartame