Construction of functional artificial minichromosomes in the fission yeast Schizosaccharomyces pombe.
The centromere DNAs from chromosomes I and III of Schizosaccharomyces pombe have been cloned in an artificial chromosome vector in both budding and fission yeasts. In S. pombe, synthetic linear and circular minichromosomes containing an intact centromere are stable mitotically and behave as independent genetic linkage groups that segregate properly through meiosis. These experiments present a general strategy for the isolation of centromeres from other organisms.
Hahnenberger
K M
KM
Department of Biological Sciences, University of California, Santa Barbara 93106.
Baum
M P
MP
Polizzi
C M
CM
Carbon
J
J
Clarke
L
L
D002503:Centromere / Q000502:physiology*; D015825:Chromosomes, Fungal / Q000502:physiology*; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics*; D005822:Genetic Vectors; D008938:Mitosis; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics; D004718:Saccharomycetales / Q000235:genetics*; D012568:Schizosaccharomyces / Q000235:genetics*
D004271:DNA, Fungal