Quantitative assessment of human neutrophil chemiluminescence induced by opsonized Escherichia coli K-12.
The interaction of opsonized E. coli K-12 bacteria and polymorphonuclear leukocytes (PMN) was quantified, using luminol-enhanced chemiluminescence (CL) as a parameter of PMN stimulation. On a double-logarithmic scale light emission depended on the opsonin concentration used during pre-opsonisation. The most potent CL-inducing agent was fresh human serum, and its stimulatory activity depended on an intact complement (C) system. Both inactivation of C by heating or blocking the classical C pathway with EGTD decreased the CL-inducing potency by a factor of 8 to 16. Hypogammaglobulinemic heated serum mediated little CL. IgG for intravenous use mediated CL generation, but reduction/alkylation and sulphitolysis reduced the stimulatory power. Evidence is presented that the anti-K-12 antibodies within commercial IgG and IgM used for substitution do not improve the stimulatory power of IgG-deficient, IgM- and C-sufficient serum, unless very high Ig concentrations are substituted.
Jungi
T W
TW
Institute of Veterinary Virology, University of Berne, Switzerland.
Schmid
A
A
Morell
A
A
Spaeth
P J
PJ
Peterhans
E
E
D000361:Agammaglobulinemia / Q000097:blood / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D004926:Escherichia coli / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000008:administration & dosage / Q000276:immunology; D007074:Immunoglobulin G / Q000008:administration & dosage / Q000276:immunology; D007075:Immunoglobulin M / Q000008:administration & dosage / Q000276:immunology; D008163:Luminescent Measurements; D009504:Neutrophils / Q000276:immunology*; D009895:Opsonin Proteins / Q000008:administration & dosage / Q000276:immunology*; D012044:Regression Analysis
D007106:Immune Sera; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D009895:Opsonin Proteins