A rapid phospholipase A2 bioassay using 14C-oleate-labelled E. coli bacterias.
Two methods of phospholipase A2 determination using 14C-labelled E. coli bacterias as substrate were compared. One method works with a filter membrane for separation of cleaved 14C-oleate from remaining phospholipids, the other uses the well-known thin-layer chromatography for lipid analysis. Some features of human serum phospholipase A2 regarding pH and Ca2+ dependency were investigated. Possible sources of errors were discussed. It was shown that either method can differentiate between normal and pathologically elevated phospholipase A2 levels, but that the filter method is superior in terms of sensitivity and workload.
Meyer
T
T
Department of Internal Medicine, University of Marburg.
von Wichert
P
P
Weins
D
D
D000208:Acute Disease; D001681:Biological Assay / Q000379:methods*; D002118:Calcium / Q000097:blood; D002250:Carbon Radioisotopes; D004926:Escherichia coli; D006801:Humans; D019301:Oleic Acid; D009829:Oleic Acids; D010195:Pancreatitis / Q000201:enzymology*; D010740:Phospholipases / Q000097:blood*; D010741:Phospholipases A / Q000097:blood*; D054467:Phospholipases A2; D012772:Shock, Septic / Q000201:enzymology*
D002250:Carbon Radioisotopes; D009829:Oleic Acids; D019301:Oleic Acid; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D002118:Calcium