title: Immunological evidence that insulin activates protein kinase C in BC3H-1 myocytes.
abstract: Effects of insulin on immunoreactive protein kinase C were examined in BC3H-1 myocytes. Insulin provoked rapid dose-dependent decreases in cytosolic enzyme, and transient increases and subsequent decreases in membrane-associated enzyme. Phorbol esters provoked similar changes. Our findings suggest that insulin provokes the translocative activation of protein kinase C.
authors.affiliation: James A. Haley Veterans' Hospital, Research Service, Tampa, FL 33612.
authors.forename: M
authors.initials: M
authors.lastname: Acevedo-Duncan
authors.forename: D R
authors.initials: DR
authors.lastname: Cooper
authors.forename: M L
authors.initials: ML
authors.lastname: Standaert
authors.forename: R V
authors.initials: RV
authors.lastname: Farese
mesh_terms: D015153:Blotting, Western; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology; D003600:Cytosol / Q000201:enzymology; D004789:Enzyme Activation / Q000187:drug effects*; D007328:Insulin / Q000494:pharmacology*; D009132:Muscles / Q000201:enzymology*; D011493:Protein Kinase C / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D007328:Insulin; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate