Ig V region gene expression in small lymphocytic lymphoma with little or no somatic hypermutation.
Using the polymerase chain reaction we examined for specific Ig kappa-L chain V region gene (V kappa gene) rearrangement in small lymphocytic non-Hodgkin's lymphomas that express Ig bearing a major kappa-L chain associated cross-reactive Id, designated 17.109. Previously, we identified the 17.109-cross-reactive Id in chronic lymphocytic leukemia as a serologic marker for expression of a highly conserved V kappa gene, designated Humkv325. Using sense-strand oligonucleotides specific for the 5'-end of this V kappa gene and antisense oligonucleotide specific for a J kappa region consensus sequence, we could amplify specifically Humkv325 when juxtaposed with J kappa through Ig gene rearrangement. This allowed us to amplify rearranged V kappa genes from DNA isolated from minute amounts of lymphoma biopsy material for molecular analyses. Our studies demonstrate that 17.109-reactive SL NHL, with or without associated CLL, rearrange, and presumably express, Humkv325 without substantial somatic diversification. Our data suggest that malignant B cells in SL NHL, in contrast to NHL of follicular center cell origin, may express immunoglobulin variable region genes with little or no somatic hypermutation.
Pratt
L F
LF
Gene Labs, Redwood City, CA.
Rassenti
L
L
Larrick
J
J
Robbins
B
B
Banks
P M
PM
Kipps
T J
TJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D004259:DNA-Directed DNA Polymerase; D005784:Gene Amplification; D015322:Gene Rearrangement, B-Lymphocyte; D005803:Genes, Immunoglobulin; D006801:Humans; D006822:Hybrid Cells / Q000032:analysis*; D007135:Immunoglobulin Variable Region / Q000235:genetics* / Q000302:isolation & purification; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics* / Q000276:immunology; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D009154:Mutation; D019914:Taq Polymerase
D007135:Immunoglobulin Variable Region; D019914:Taq Polymerase; D004259:DNA-Directed DNA Polymerase