Intraventricular xenografts: chronic injection of antibodies into the CSF provokes granulomatosis reactions but Ia antibodies do not enhance graft survival.
We have studied the influence of chronic infusions of antibodies against Ia antigen into the CSF on immunological reactions to intraventricular xenografts in rats. The antibodies were infused with osmotic pumps implanted one day before grafting. One set of control groups consisted of animals with xenografts which simultaneously received antibody against penicillin or vehicle with no antibody. In a second set of control groups, antibodies against Ia antigen, penicillin, rat anti-mouse IgG or vehicle (no antibody) were administered alone, without grafts. In the animals that received infusions of antibodies against either Ia antigen or penicillin plus xenografts, intense granulomatosis reactions were observed in the host brains. These reactions were not observed in the animals that received antibody infusions without xenografts. It appears that this reaction was produced by the massive and persistent injection of immunoglobulins, together with the presence of xenograft tissue. The chronic infusion of Ia antibodies did not prolong xenograft survival.
Poltorak
M
M
National Institute of Mental Health, Neurosciences Center at St. Elizabeths, Washington, DC 20032.
Logan
S
S
Freed
W J
WJ
D000818:Animals; D000906:Antibodies / Q000276:immunology; D001921:Brain / Q000196:embryology; D002552:Cerebral Ventricles / Q000601:surgery*; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D007276:Injections, Intraventricular; D008297:Male; D009417:Nerve Tissue / Q000637:transplantation*; D010406:Penicillins / Q000276:immunology; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D014183:Transplantation, Heterologous / Q000276:immunology*
D000906:Antibodies; D000949:Histocompatibility Antigens Class II; D010406:Penicillins