The beta form is the dominant interleukin 1 released by murine peritoneal macrophages.
Using highly specific polyclonal antisera raised against recombinant murine IL-1 alpha and beta, we performed solid-phase immunoabsorption studies on supernates of resident and adjuvant-elicited CBA/J mouse peritoneal macrophages. Antibody specificity was established by reciprocal absorption studies and Western blot analysis. Supernates obtained from macrophages cultured for 18 hr in the presence of 1 microgram/ml lipopolysaccharide (LPS) were subjected to immunoabsorption. Approximately 78-90% of the released bioactive material was IL-1 and about 80% of this could be attributed to IL-1 beta. Analogous to that reported for human monocytes, these data suggest that IL-1 beta is the predominant released form of IL-1.
Chensue
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SW
Department of Pathology, University of Michigan Medical School, Ann Arbor 48109.
Shmyr-Forsch
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C
Otterness
I G
IG
Kunkel
S L
SL
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D002846:Chromatography, Affinity; D005260:Female; D007163:Immunosorbent Techniques; D007375:Interleukin-1 / Q000032:analysis / Q000276:immunology / Q000502:physiology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008808:Mice, Inbred CBA; D010529:Peritoneal Cavity / Q000166:cytology*
D000906:Antibodies; D007375:Interleukin-1; D008070:Lipopolysaccharides