title: Production scale purification of biosynthetic human insulin by reversed-phase high-performance liquid chromatography.
abstract: A process based on reversed-phase high-performance liquid chromatography (RP-HPLC) has been developed for the purification of biosynthetic human insulin (BHI). The RP-HPLC procedure has been successfully integrated into the multimodal chromatographic production process used to purify kilogram quantities of BHI. Axial compression column technology was used in the scale-up process. The RP-HPLC procedure yields an insulin product having high chemical purity and full biological activity.
authors.affiliation: Department of Biosynthetic Isolation and Purification Development, Lilly Research Laboratories, Indianapolis, IN 46285.
authors.forename: E P
authors.initials: EP
authors.lastname: Kroeff
authors.forename: R A
authors.initials: RA
authors.lastname: Owens
authors.forename: E L
authors.initials: EL
authors.lastname: Campbell
authors.forename: R D
authors.initials: RD
authors.lastname: Johnson
authors.forename: H I
authors.initials: HI
authors.lastname: Marks
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000032:analysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification*; D010455:Peptides / Q000032:analysis; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification; D013056:Spectrophotometry, Ultraviolet
chemical_list: D007328:Insulin; D010455:Peptides; D011994:Recombinant Proteins