title: Microscopic observation of leukocyte kinesis in the vascular bed during hemodialysis using the rabbit ear chamber technique.
abstract: Leukocyte kinesis in the capillary vascular bed during hemodialysis (HD) was investigated to elucidate the mechanism of transient leukopenia. Leukocyte movement was observed microscopically during HD using the rabbit ear chamber (REC) technique, which permits visualization of the movement of blood corpuscles in capillaries. Blood was drawn from the femoral artery and returned into the auricular and/or carotid artery so that the blood passing through the hollow fiber artificial kidney (HFAK) flowed into capillaries in the REC. Leukocyte counts of blood samples taken from the afferent and efferent limbs of the HD circuit, the right jugular vein and the right atrium were determined consecutively during HD. The difference in the leukocyte count was observed between the afferent and efferent limbs for the first 15 minutes and thereafter between the efferent limb and the jugular vein. The "transpulmonary" difference in the leukocyte count was not noticed throughout HD. Between 15 and 90 minutes after the start of HD, scarcely any circulating leukocytes were found in capillaries in the REC and some leukocytes were attached to the endothelial surface. Thereafter circulating leukocytes were seen again and detachment of leukocytes from the endothelial surface was observed. No leukocyte aggregation or embolization of aggregating leukocytes was noticed. This evidence suggests that leukopenia may be attributed to the transient shift of leukocytes to the marginal pool of the vessel lumen and this process may not be specific for the pulmonary vasculature, but may occur in the first capillary bed into which the blood passing through the HFAK flows.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Kidney Center, Tokyo Women's Medical College, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Teraoka
authors.forename: M
authors.initials: M
authors.lastname: Sugawara
authors.forename: Y
authors.initials: Y
authors.lastname: Kitano
authors.forename: T
authors.initials: T
authors.lastname: Hoshino
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
authors.forename: Y
authors.initials: Y
authors.lastname: Minagawa
authors.forename: S
authors.initials: S
authors.lastname: Naganuma
authors.forename: T
authors.initials: T
authors.lastname: Sanaka
authors.forename: M
authors.initials: M
authors.lastname: Mineshima
authors.forename: K
authors.initials: K
authors.lastname: Era
mesh_terms: D000818:Animals; D002196:Capillaries / Q000033:anatomy & histology; D002464:Cell Migration Inhibition; D004431:Ear, External / Q000098:blood supply; D004730:Endothelium, Vascular / Q000033:anatomy & histology; D007958:Leukocyte Count; D007962:Leukocytes / Q000276:immunology*; D011817:Rabbits; D006435:Renal Dialysis; D012884:Skin Window Technique