[The structure of oligosaccharide fragments of glycoproteins from influenza virus A/Krasnodar/101/59/(H2N2)--heavy and light chains of hemagglutinin and neuraminidase].
The structure and heterogeneity of carbohydrate chains of hemagglutinin (HA) and neuraminidase (NA), the surface glycoproteins of influenza virus A/Krasnodar/101/59 (H2N2), were investigated. Hemagglutinin was reduced with beta-mercaptoethanol and its heavy (HA1) and light (HA2) chains were separated by gel chromatography. Amino acid and sugar composition of HA1, HA2 and NA was elucidated. The carbohydrate chains of the glycoproteins were cleaved off by the alkaline LiBH4 treatment and oligosaccharides were reduced with NaB[3H]4. They were fractionated by subsequent two-step HPLC on Ultrasphere-C8 and Zorbax-NH2 columns with simultaneous identification using nonlabelled oligosaccharides of known structures. Some of the major oligosaccharides isolated from HA1, HA2 and NA were thus identified as high mannose chains, containing 5-9 mannose residues, and complex chains, first of all biantennary chains having or not having bisecting N-acetylglucosamine and/or fucose residues. The approach which has been developed enables one to study the structure and heterogeneity of carbohydrate chains starting from one nmole of a desialylated N-glycoprotein.
Arbatskiĭ
N P
NP
Zheltova
A O
AO
Iurtov
D V
DV
Derevitskaia
V A
VA
Kochetkov
N K
NK
D000596:Amino Acids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D006023:Glycoproteins / Q000032:analysis*; D006389:Hemagglutinins, Viral / Q000032:analysis*; D053121:Influenza A Virus, H2N2 Subtype; D009980:Influenza A virus / Q000032:analysis* / Q000201:enzymology / Q000276:immunology; D009439:Neuraminidase / Q000032:analysis*; D009844:Oligosaccharides / Q000032:analysis*
D000596:Amino Acids; D006023:Glycoproteins; D006389:Hemagglutinins, Viral; D009844:Oligosaccharides; D009439:Neuraminidase
Struktura oligosakharidnykh fragmentov glikoproteinov virusa grippa A/Krasnodar/101/59(H2N2)--tiazheloĭ i legkoĭ tsepeĭ gemaggliutinina i neĭraminidazy.