Genetic characterization of frameshift suppressors with new decoding properties.
Suppressor mutants that cause ribosomes to shift reading frame at specific and new sequences are described. Suppressors for trpE91, the only known suppressible -1 frameshift mutant, have been isolated in Escherichia coli and in Salmonella typhimurium. E. coli hopR acts on trpE91 within the 9-base-pair sequence GGA GUG UGA, is dominant, and is located at min 52 on the chromosome. Its Salmonella homolog maps at an equivalent position and arises as a rarer class in that organism as compared with E. coli. The Salmonella suppressor, hopE, believed to be in a duplicate copy of the same gene, maps at min 17. The +1 suppressor, sufT, acts at the nonmonotonous sequence CCGU, is dominant, and maps at min 59 on the Salmonella chromosome.
Hughes
D
D
Department of Genetics, Trinity College, Dublin, Ireland.
Thompson
S
S
O'Connor
M
M
Tuohy
T
T
Nichols
B P
BP
Atkins
J F
JF
D001483:Base Sequence; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005838:Genotype; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids; D012486:Salmonella typhimurium / Q000235:genetics*; D013489:Suppression, Genetic; D014161:Transduction, Genetic