title: Glomerular actions of ANG II during reduction of renal artery pressure: a morphometric analysis.
abstract: The glomeruli of kidneys subjected to reduced perfusion pressure were examined morphometrically. The left renal artery was narrowed for 30 min in anesthetized dogs with (n = 6) or without (n = 7) converting-enzyme inhibition (captopril). The kidneys were then rapidly fixed by glutaraldehyde perfusion at high flow rate. In a comparison of glomeruli of kidneys subjected to pressure reduction in captopril-treated and untreated dogs, there was significantly greater mesangial contraction in the latter, but morphometric analysis revealed no significant differences in the glomerular surface area available for filtration as evidenced by glomerular capillary volume fractions, surface areas of the filtering basement membrane between epithelial and endothelial cells, or the length densities of the glomerular epithelial slits. In a comparison of the left (pressure reduction) and right (no pressure reduction) kidneys in the captopril-treated dogs, there was no significant effect of reduction of renal perfusion pressure per se on mesangial contraction or glomerular filtration surface area when angiotensin (ANG) II formation was blocked. Thus ANG II caused mesangial cell contraction after renal artery stenosis, but this did not significantly change glomerular ultrafiltration surface area.
authors.affiliation: Emily Stewart Renal Laboratory, Baker Medical Research Institute, Melbourne, Australia.
authors.forename: W P
authors.initials: WP
authors.lastname: Anderson
authors.forename: D
authors.initials: D
authors.lastname: Alcorn
authors.forename: A I
authors.initials: AI
authors.lastname: Gilchrist
authors.forename: J M
authors.initials: JM
authors.lastname: Whiting
authors.forename: G B
authors.initials: GB
authors.lastname: Ryan
mesh_terms: D000804:Angiotensin II / Q000502:physiology*; D000818:Animals; D001485:Basement Membrane / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D001794:Blood Pressure / Q000187:drug effects; D002216:Captopril / Q000494:pharmacology*; D004285:Dogs; D007678:Kidney Glomerulus / Q000502:physiology* / Q000648:ultrastructure; D012016:Reference Values; D012077:Renal Artery / Q000502:physiology*; D014655:Vascular Resistance
chemical_list: D000804:Angiotensin II; D002216:Captopril