Synthesis of radiolabeled acetyl-coenzyme A from sodium acetate.
The synthesis of high specific radioactivity [14C]-acetyl-Coenzyme A from [14C]sodium acetate, 2,6-dichlorobenzoic acid, 1,1'-carbonyldiimidazole, and CoA is reported. Starting with 1 mumol of [14C]sodium acetate, this method yields pure [14C]acetyl-CoA in yields approaching 40%. Chromatography on a reversed-phase ODS column was used to separate acetyl-CoA from Coenzyme A and side products. The acetylating agent is apparently a reaction intermediate, acetylimidazole.
Clough
R C
RC
Department of Botany, Miami University, Oxford, Ohio 45056.
Barnum
S R
SR
Jaworski
J G
JG
D000085:Acetates; D019342:Acetic Acid; D000105:Acetyl Coenzyme A / Q000138:chemical synthesis* / Q000302:isolation & purification; D002250:Carbon Radioisotopes; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007202:Indicators and Reagents; D011865:Radioisotope Dilution Technique; D013053:Spectrophotometry / Q000379:methods
D000085:Acetates; D002250:Carbon Radioisotopes; D007202:Indicators and Reagents; D000105:Acetyl Coenzyme A; D019342:Acetic Acid