Induction of protein kinase C translocation and cell differentiation in ML-1 human myeloblastic leukemic cells by tumor necrosis factor-alpha, transforming growth factor-beta, or tetradecanoylphorbol acetate.
Tumor necrosis factor-alpha and transforming growth factor-beta, like 12-O-tetradecanoylphorbol 13-acetate, induce differentiation of ML-1 human myeloblastic leukemia cells along the monocyte path. As measured at 5 min following exposure of the cells to either of these agents, extensive translocation of protein kinase C from the cytosolic to the membrane fraction occurred. A correlation was observed to exist between protein kinase C translocation, cell differentiation, and cessation of cell growth induced by transforming growth factor-beta and tumor necrosis factor-alpha.
Guan
X P
XP
Department of Experimental Therapeutics, Roswell Park Memorial Institute, Buffalo, New York 14263.
Hromchak
R A
RA
Bloch
A
A
D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D004790:Enzyme Induction; D006801:Humans; D007700:Kinetics; D015470:Leukemia, Myeloid, Acute; D011493:Protein Kinase C / Q000096:biosynthesis* / Q000235:genetics; D011499:Protein Processing, Post-Translational / Q000187:drug effects*; D011994:Recombinant Proteins / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D015291:Transforming Growth Factors / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D015291:Transforming Growth Factors; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate