[Characterization of a chondrocyte primary culture from rib cartilage of the rat].
In order to standardize and to characterize a chondrocyte primary culture, cells from rat rib resting cartilage were used. High yield (0.99 +/- 0.18 x 10(6) cells/rat) and viability (91.76%) of costal cartilage cells was reached by enzymatic digestion with collagenase. The cells were cultivated in Dulbecco's medium (DME) supplemented with 10%. Heat inactivated newborn calf serum, at 37 degrees under humidified atmosphere of 5% CO2 in air. Two or three days after plating, the cells were attached to the surface of tissue culture weel, and began dividing. Adhesion was independent of plating density. The doubling time of cell population was found to be 23.19 hours. The cells became a monolayer and required easy maintenance. The results support the contention that rat costal cartilage is a good source of chondrocytes for primary culture cells experiments.
Favaretto
A L
AL
Lins
C E
CE
Felipe
M S
MS
Da Cruz
W B
WB
D000818:Animals; D002356:Cartilage / Q000166:cytology*; D002448:Cell Adhesion; D002452:Cell Count; D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media; D006132:Growth Plate / Q000166:cytology*; D008297:Male; D051381:Rats; D012272:Ribs
D003470:Culture Media
Caracterização da cultura primária de condrócitos a partir da cartilagem de costela de ratos.