title: The neutrophil-activating protein (NAP-1) is also chemotactic for T lymphocytes.
abstract: T lymphocyte chemotactic factor (TCF) was purified to homogeneity from the conditioned media of phytohemagglutinin-stimulated human blood mononuclear leukocytes by a sequence of chromatography procedures. The amino-terminal amino acid sequence of the purified TCF showed identity with neutrophil-activating protein (NAP-1). Both TCF and recombinant NAP-1 (rNAP-1) were chemotactic for neutrophils and T lymphocytes in vitro supporting the identity of TCF with NAP-1. Injection of rNAP-1 into lymphatic drainage areas of lymph nodes in Fisher rats caused accelerated emigration of only lymphocytes in high endothelial venules. Intradermal injection of rNAP-1 caused dose-dependent accumulation of neutrophils and lymphocytes.
authors.affiliation: Laboratory of Molecular Immunoregulation, National Cancer Institute, Frederick, MD 21701.
authors.forename: C G
authors.initials: CG
authors.lastname: Larsen
authors.forename: A O
authors.initials: AO
authors.lastname: Anderson
authors.forename: E
authors.initials: E
authors.lastname: Appella
authors.forename: J J
authors.initials: JJ
authors.lastname: Oppenheim
authors.forename: K
authors.initials: K
authors.lastname: Matsushima
mesh_terms: D000818:Animals; D002630:Chemotactic Factors / Q000302:isolation & purification*; D002634:Chemotaxis, Leukocyte; D016209:Interleukin-8; D010455:Peptides / Q000302:isolation & purification*; D051381:Rats; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D002630:Chemotactic Factors; D016209:Interleukin-8; D010455:Peptides