Endothelin-induced mobilization of Ca2+ and the possible involvement of platelet activating factor and thromboxane A2.
Endothelin (ET) caused transient and sustained elevations of cytosolic free Ca2+ concentrations ([Ca2+]i) in cultured rat and rabbit vascular smooth muscle cells (VSMC). Specific platelet activating factor (PAF) antagonists (CV-6209 and WEB-2086) and arachidonic acid (AA) cascade blockers (chlorpromazine, indomethacin, CV-4151 and AA-2414) potently inhibited the ET-induced increase in [Ca2+]i. Additionally, these compounds inhibited the PAF-induced increase in [Ca2+]i. These results suggest that PAF and thromboxane A2 (TXA2) may be involved in the mechanism of ET-induced mobilization of Ca2+ in cultured rat and rabbit VSMC.
Takayasu
M
M
Central Research Division, Takeda Chemical Industries, Ltd., Osaka, Japan.
Kondo
K
K
Terao
S
S
D000818:Animals; D001572:Benzofurans; D002118:Calcium / Q000096:biosynthesis / Q000378:metabolism*; D002478:Cells, Cultured; D016232:Endothelins; D004730:Endothelium, Vascular / Q000378:metabolism; D005456:Fluorescent Dyes; D016257:Fura-2; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000378:metabolism; D010455:Peptides / Q000494:pharmacology*; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors / Q000502:physiology*; D011726:Pyridinium Compounds / Q000494:pharmacology; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D013928:Thromboxane A2 / Q000502:physiology*
D001572:Benzofurans; D016232:Endothelins; D005456:Fluorescent Dyes; D010455:Peptides; D010972:Platelet Activating Factor; D011726:Pyridinium Compounds; C053321:CV 6209; D013928:Thromboxane A2; D002118:Calcium; D016257:Fura-2