title: In vivo localization of a bispecific antibody which targets human T lymphocytes to lyse human colon cancer cells.
abstract: A bispecific MAb was derived from the fusion of a hybridoma producing MAb CD3 with a hybridoma producing MAb L-DI (which is directed against a 41-kDa glycoprotein expressed in most gastro-intestinal and pancreatic carcinomas). Bispecific antibody molecules were isolated from parental antibody molecules by the use of hydroxylapatite-HPLC and shown to target human cytolytic T lymphocytes, irrespective of their original specificity, to specifically lyse human colon carcinoma cells. Localization studies in vivo using nude mice bearing human colon carcinoma xenografts showed significant accumulation of the HPLC-purified 125I-labelled bispecific antibodies into the tumor compared to 131I-labelled control CD3 antibody.
authors.affiliation: Ludwig Institute for Cancer Research, Lausanne Branch, Basel, Switzerland.
authors.forename: I G
authors.initials: IG
authors.lastname: Barr
authors.forename: S
authors.initials: S
authors.lastname: Miescher
authors.forename: V
authors.initials: V
authors.lastname: von Fliedner
authors.forename: F
authors.initials: F
authors.lastname: Buchegger
authors.forename: C
authors.initials: C
authors.lastname: Barras
authors.forename: A
authors.initials: A
authors.lastname: Lanzavecchia
authors.forename: J P
authors.initials: JP
authors.lastname: Mach
authors.forename: S
authors.initials: S
authors.lastname: Carrel
mesh_terms: D000818:Animals; D000912:Antibodies, Neoplasm / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003110:Colonic Neoplasms / Q000276:immunology*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D000912:Antibodies, Neoplasm