The mast cell-specific expression of a protease gene, RMCP II, is regulated by an enhancer element that binds specifically to mast cell trans-acting factors.
The rat mast cell protease gene, RMCP II, is specifically expressed in the mucosal subclass of rat mast cells. We show here that the 5'-flanking region of this gene contains a mast cell-specific enhancer that directs preferential expression of a linked reporter gene (human growth hormone) transfected into rat basophilic leukemia cells. A DNA fragment containing the enhancer sequence is capable of binding specifically to mast cell nuclear trans-acting factors. The sequence of this enhancer element contains a region of homology to a consensus core sequence present in the enhancer region of the pancreatic protease genes.
Sarid
J
J
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
Benfey
P N
PN
Leder
P
P
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002872:Chromosome Deletion; D004742:Enhancer Elements, Genetic; D005796:Genes; D005809:Genes, Regulator; D013006:Growth Hormone / Q000235:genetics; D006801:Humans; D015471:Leukemia, Basophilic, Acute / Q000201:enzymology / Q000235:genetics; D008407:Mast Cells / Q000201:enzymology*; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases / Q000235:genetics*; D051381:Rats; D014157:Transcription Factors / Q000378:metabolism*; D014162:Transfection
D014157:Transcription Factors; D013006:Growth Hormone; D010447:Peptide Hydrolases