title: Changes in the proteolytic activity of human erythrocyte membrane during red cell aging.
abstract: The action of endogenous membrane proteinases in membranes isolated from human red cells of various ages was assayed by three groups of methods: (1) determination of the amount of protein fragments released to the acid-soluble fraction; (2) monitoring of changes in ESR spectra of maleimide spin-labeled erythrocyte membranes; (3) electrophoretic methods: a two-dimensional analysis and analysis of the activity inside SDS-PAGE gels. For all the methods the effects of proteinase action were highest in ghosts isolated from the erythrocytes of middle age.
authors.affiliation: Department of Biophysics, University of Lódź, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Gaczyńska
mesh_terms: D002364:Caseins / Q000378:metabolism; D004905:Erythrocyte Aging; D004910:Erythrocyte Membrane / Q000201:enzymology*; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008565:Membrane Proteins / Q000097:blood; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000097:blood*; D013113:Spin Labels
chemical_list: D002364:Caseins; D008565:Membrane Proteins; D013113:Spin Labels; D010447:Peptide Hydrolases