title: Rapid S-phase determination of non-Hodgkin's lymphomas with the use of an immunofluorescence bromodeoxyuridine labeling index procedure.
abstract: Cell kinetic measurements are currently being investigated to determine if they are useful in the clinical management of patients with non-Hodgkin's lymphomas (NHLs). Although the tritiated thymidine labeling index (TLI) is the standard method of S-phase measurement, it is difficult to perform. The authors describe a slide-based immunofluorescence labeling index (LI) method that uses 5-bromo-2-deoxyuridine (BrdUrd) as the pulsing medium and a monoclonal antibody (BU-1) to BrdUrd. The BrdUrd LI was performed on 217 NHLs and compared with routine histologic results. The authors found a median BrdUrd LI of 0.9% for low-grade NHLs; 7.5% for intermediate-grade; 10.4% for high-grade; and 2.2% for T-cell NHLs. This method provides a rapid, reliable S-phase measurement that can be easily performed in the clinical laboratory. It should replace the TLI and allow wider application of S-phase measurements in the NHL.
authors.affiliation: Department of Laboratory Medicine, Mayo Clinic, Rochester, Minnesota 55905.
authors.forename: T E
authors.initials: TE
authors.lastname: Witzig
authors.forename: N J
authors.initials: NJ
authors.lastname: Gonchoroff
authors.forename: P R
authors.initials: PR
authors.lastname: Greipp
authors.forename: J A
authors.initials: JA
authors.lastname: Katzmann
authors.forename: M J
authors.initials: MJ
authors.lastname: Stenson
authors.forename: T M
authors.initials: TM
authors.lastname: Habermann
authors.forename: J P
authors.initials: JP
authors.lastname: Colgan
authors.forename: T M
authors.initials: TM
authors.lastname: Therneau
authors.forename: P M
authors.initials: PM
authors.lastname: Banks
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D001973:Bromodeoxyuridine / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D007399:Interphase; D008198:Lymph Nodes / Q000473:pathology; D008228:Lymphoma, Non-Hodgkin / Q000473:pathology*; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D001973:Bromodeoxyuridine