Differential extra-renal expression of the mouse renin genes.
We have used RNase-protection analyses to study renin gene expression in one- and two-gene mouse strains. The RNase-protection assay is capable of discriminating between the transcripts from the different renin genes. In a two-gene strain containing Ren-1D and Ren-2, we demonstrate transcriptional activity from Ren-1D in kidney, submandibular gland (SMG), testes, liver, brain and heart. Ren-2 is clearly expressed in kidney, SMG and testes. Similar analyses of one gene strains (containing Ren-1C only) show expression in kidney, SMG, testes, brain and heart. We cannot detect renin mRNA in the liver of these mice. Ren-1C and Ren-1D thus display quite different tissue-specificities. In order to determine whether the different tissue-specificities of the highly homologous Ren-1C and Ren-1D genes are due to different trans-acting factors in the different mouse strains or to different cis-acting DNA elements inherent to the genes, we introduced a Ren-1D transgene (Ren-1*) into a background strain containing only the Ren-1C gene. The transgene exhibits the same tissue-specificity as the Ren-1D gene of two-gene strains suggesting the presence of different cis-acting DNA elements in Ren-1C and Ren-1D.
Miller
C C
CC
Department of Biochemistry, University of Leicester, UK.
Carter
A T
AT
Brooks
J I
JI
Lovell-Badge
R H
RH
Brammar
W J
WJ
D000818:Animals; D015139:Blotting, Southern; D005786:Gene Expression Regulation; D007668:Kidney / Q000502:physiology; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics*; D008822:Mice, Transgenic / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D012083:Renin / Q000235:genetics*; D015183:Restriction Mapping; D014018:Tissue Distribution
D012333:RNA, Messenger; D012083:Renin