title: Fluorescent antibodies in polymyositis using cultured human skin fibroblasts: granular perinuclear cytoplasmic staining pattern by sera from patients with polymyositis and pulmonary fibrosis.
abstract: Immunofluorescent antibodies were studied in sera from 48 patients with polymyositis (PM) and 149 control subjects using cultured human skin fibroblasts as substrate. Forty-six (95.8%) of PM sera showed a positive reaction. Among them, a characteristic granular perinuclear cytoplasmic staining was seen in 8 sera, including 4 with anti-Jo-1. Four of these 8 sera showed positive staining on HEp-2 cells with the same pattern. All patients showing this pattern of staining had pulmonary fibrosis which was not seen in control patients. Consequently, this staining pattern might be a specific marker for a subset of patients with PM with pulmonary fibrosis.
authors.affiliation: Fourth Department of Medicine, School of Medicine, Toho University, Tokyo, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Saito
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshimoto
authors.forename: H
authors.initials: H
authors.lastname: Oshima
authors.forename: H
authors.initials: H
authors.lastname: Yoshida
authors.forename: M
authors.initials: M
authors.lastname: Kinoshita
mesh_terms: D000328:Adult; D001323:Autoantibodies / Q000032:analysis*; D001324:Autoantigens / Q000276:immunology; D002478:Cells, Cultured; D003095:Collagen Diseases / Q000276:immunology; D003594:Cytoplasmic Granules / Q000276:immunology*; D005260:Female; D005347:Fibroblasts / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D008875:Middle Aged; D009136:Muscular Dystrophies / Q000276:immunology; D009220:Myositis / Q000150:complications / Q000276:immunology*; D011658:Pulmonary Fibrosis / Q000150:complications / Q000276:immunology*; D012867:Skin
chemical_list: D001323:Autoantibodies; D001324:Autoantigens