title: Immunologic regulation of E. coli K1 by serum from neonatal rats is enhanced following intraperitoneal administration of human IgG.
abstract: The effect of 1500 mg/kg intraperitoneal human IgG on the capacity of neonatal rat serum to opsonize and to kill Escherichia coli K1 and to deposit IgG and C3 onto this organism was investigated. Unlike serum from neonatal rats injected with albumin, serum from neonatal rats injected with human IgG opsonized and killed E. coli K1 efficiently. Heat treatment abolished the bactericidal effect of serum from IgG recipients, suggesting a role for complement. A radioimmunobinding assay demonstrated that the capacity of neonatal rat serum to deposit IgG and C3 onto E. coli K1 was impaired. However, intraperitoneal human IgG enabled serum from neonatal rats to deposit human IgG onto the bacteria and enhanced C3 deposition to a level equivalent to that observed with adult rat serum. Therefore, neonatal rats have a functionally competent classic pathway of complement. Human IgG ameliorates the opsonic and bacteriolytic inadequacies of neonatal rat serum that result primarily from a deficiency of antibodies to E. coli.
authors.affiliation: Department of Pediatrics, University of Utah School of Medicine, Salt Lake City.
authors.forename: H A
authors.initials: HA
authors.lastname: Lassiter
authors.forename: R D
authors.initials: RD
authors.lastname: Christensen
authors.forename: C J
authors.initials: CJ
authors.lastname: Parker
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000276:immunology*; D000907:Antibodies, Bacterial / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D001770:Blood Bactericidal Activity; D003167:Complement Activation; D003176:Complement C3 / Q000378:metabolism; D004926:Escherichia coli / Q000276:immunology*; D006358:Hot Temperature; D007074:Immunoglobulin G / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology; D009895:Opsonin Proteins; D010587:Phagocytosis; D051381:Rats
chemical_list: D000907:Antibodies, Bacterial; D003176:Complement C3; D007074:Immunoglobulin G; D009895:Opsonin Proteins