Modification of the biosynthesis and composition of polyglycerophosphatides in outer and inner mitochondrial membranes by cytidine liponucleotides.
The biosynthesis of [3H]polyglycerophosphatides ([3H]phosphatidylglycerophosphate and [3H]phosphatidylglycerol) in mitochondrial and submitochondrial (outer and inner) membranes isolated from guinea pig liver was examined. Experimental results have established that the amount of biosynthesized [3H]polyglycerophosphatides and the relative amounts of biosynthesized [3H]phosphatidylglycerol and [3H]phosphatidylglycerolphosphate can be influenced by varying the composition of fatty acids in CDP-diglycerides and by altering the incubation time of the mixture containing CDP-diglycerides (obligatory precursor), sn-[2-3H]glycerol-3-phosphate and mitochondria or submitochondrial membranes. The changes thus obtained in respect to the amount and composition of biosynthesized [3H]polyglycerophosphatides are different in mitochondria and submitochondrial membranes. The highest amount of biosynthesized [3H]polyglycerophosphatides was obtained with CDP-didecanoin and inner mitochondrial membranes. The greatest accumulation of [3H]phosphatidylglycerol with CDP-didecanoin was obtained in mitochondria and outer mitochondrial membranes, while in inner mitochondrial membranes the amounts of [3H]phosphatidylglycerol and [3H]phosphatidylglycerolphosphate accumulated were approximately the same. In general, prolongation of the incubation time decreased the relative amounts of [3H]phosphatidylglycerolphosphate and increased the amount of accumulated [3H]phosphatidylglycerol, but the absolute amounts of these [3H]polyglycerophosphatides were more dependent on fatty acid composition of CDP-diglycerides tested. The following cytidine liponucleotides were tested: CDP-didecanoin, CDP-dipalmitin, CDP-diolein, and CDP-diglycerides containing saturated and unsaturated fatty acids similar to those in egg yolk lecithin. The formation of [3H]cardiolipin from [3H]phosphatidylglycerol in the presence of CDP-didecanoin and Mn2+ was found in both the outer and inner mitochondrial membranes.
Stuhne-Sekalec
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L
Department of Clinical Biochemistry, Banting Institute, Faculty of Medicine, University of Toronto, Canada.
Stanacev
N Z
NZ
D000818:Animals; D002308:Cardiolipins / Q000378:metabolism*; D003565:Cytidine Diphosphate / Q000031:analogs & derivatives / Q000378:metabolism*; D003567:Cytidine Diphosphate Diglycerides / Q000378:metabolism; D003597:Cytosine Nucleotides / Q000378:metabolism*; D003651:Decanoates / Q000378:metabolism; D004075:Diglycerides / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D005989:Glycerides / Q000378:metabolism*; D005994:Glycerophosphates / Q000378:metabolism; D006168:Guinea Pigs; D007425:Intracellular Membranes / Q000378:metabolism; D008930:Mitochondria, Liver / Q000378:metabolism* / Q000648:ultrastructure; D010715:Phosphatidylglycerols / Q000096:biosynthesis*; D013997:Time Factors; D014316:Tritium
D002308:Cardiolipins; D003567:Cytidine Diphosphate Diglycerides; D003597:Cytosine Nucleotides; D003651:Decanoates; D004075:Diglycerides; D005227:Fatty Acids; D005989:Glycerides; D005994:Glycerophosphates; D010715:Phosphatidylglycerols; C034358:phosphatidylglycerophosphate; D014316:Tritium; D003565:Cytidine Diphosphate; C065627:cytidine diphosphate-didecanoin