[A new in vitro test of endothelial viability. Vital staining using Janus green with spectrophotometric analysis of the color extract].
A new in vitro technique has been developed to measure by simple photometry the amount of Janus Green extracted with absolute alcohol from isolated corneas after vital staining. The amount of stain corresponds to the percentage of damaged endothelial cells and is a reliable parameter of endothelial viability. More rapid and easier to perform than the usual cell counting of devitalized cells this technique may be used to study the endothelial damage induced e.g. by experimental surgery, intraocular solutions, IOL material, cornea storage solutions.
Hartmann
C
C
Rieck
P
P
Pharmakakis
N
N
Bergmann
L
L
D000818:Animals; D001391:Azo Compounds; D002470:Cell Survival; D004728:Endothelium, Corneal / Q000293:injuries / Q000473:pathology / Q000503:physiopathology*; D013053:Spectrophotometry / Q000379:methods*; D013552:Swine
D001391:Azo Compounds; C047446:janus green
Un nouveau test in vitro de la viabilité endothéliale. La coloration vitale au vert de Janus avec analyse spectrophotométrique du colorant extrait.