The growth of Rauscher erythroleukemia cells is mediated by autocrine production of a factor with biological activity similar to interleukin-3.
Under serum-deprived and chemically defined culture conditions, the growth of Rauscher erythroleukemia cells is mediated by an autocrine mechanism. The growth-promoting activity is produced by fresh or irradiated cells and resembles the activity of interleukin-3 (IL-3) in its ability to sustain colony formation from three of four IL-3-dependent cell lines and to induce formation of granulocyte/macrophage (GM) colonies and, in the presence of erythropoietin (Ep), of erythroid bursts and mixed erythroid colonies. IL-3, IL-1, IL-4, IL-6, GM colony-stimulating factor (GM-CSF), G-CSF, M-CSF, Ep, and media conditioned by concanavalin A-stimulated mouse spleen cells or phytohemagglutinin-stimulated LBRM 33 cells were unable to induce proliferation of the Rauscher erythroleukemia cells. Northern analysis of total and polyA-selected RNA extracted from untreated Rauscher cells or from cells 24 hours after irradiation showed the presence of message for M-CSF but not for IL-3, IL-1, GM-CSF, or G-CSF. The production of IL-6 was excluded by a sensitive bioassay. These results indicate that the autocrine growth of the Rauscher cell line is mediated by a growth factor different from IL-3, but with similar biological activity. Activation of the expression of such a growth factor during viral infection may contribute to the generation of leukemic cells that have the property to grow in vitro and generate Rauscher erythroleukemia cell lines.
Migliaccio
G
G
Department of Medicine, University of Washington, Seattle 98195.
Migliaccio
A R
AR
Ruscetti
S
S
Adamson
J W
JW
D000818:Animals; D015152:Blotting, Northern; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003470:Culture Media; D005312:Fetal Blood / Q000502:physiology; D006133:Growth Substances / Q000302:isolation & purification; D006410:Hematopoiesis / Q000187:drug effects; D016298:Hematopoietic Cell Growth Factors; D006412:Hematopoietic Stem Cells / Q000502:physiology; D007377:Interleukin-3 / Q000096:biosynthesis* / Q000502:physiology; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism / Q000473:pathology*; D008297:Male; D051379:Mice; D011925:Rauscher Virus
D003470:Culture Media; D006133:Growth Substances; D016298:Hematopoietic Cell Growth Factors; D007377:Interleukin-3