title: Visualization of eosinophil chemotactic factor in abomasal tissue of cattle by immunoperoxidase staining during Ostertagia ostertagi infection.
abstract: Eosinophil chemotactic factor (ECF) was localized predominantly in the intestinal cells and lateral hypodermal cords of developing fifth stage larvae (L5) of Ostertagia ostertagi within abomasal tissue cross-sections by peroxidase in an antibody sandwich technique using monoclonal antibody to ECF. Cooperia oncophora larvae in tissue cross-sections did not stain using this technique. These experiments demonstrate that ECF is localized in Ostertagia ostertagi organelles and is probably released by the developing L5 into the abomasal tissue surrounding the parasitized gland. The presence of ECF within O. ostertagi larvae in situ and the results of previous experiments demonstrated in vitro and in vivo ECF chemotactic activity help to explain why eosinophils are observed histologically in abomasal tissues from cattle with ostertagiasis.
authors.affiliation: Animal Parasite Research Laboratory, U.S. Department of Agriculture, Auburn, AL 36830.
authors.forename: P H
authors.initials: PH
authors.lastname: Klesius
authors.forename: T G
authors.initials: TG
authors.lastname: Snider
authors.forename: L W
authors.initials: LW
authors.lastname: Horton
authors.forename: C H
authors.initials: CH
authors.lastname: Crowder
mesh_terms: D000018:Abomasum / Q000032:analysis*; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology*; D002630:Chemotactic Factors / Q000032:analysis*; D002631:Chemotactic Factors, Eosinophil / Q000032:analysis*; D002634:Chemotaxis, Leukocyte; D004804:Eosinophils / Q000276:immunology; D007124:Immunoenzyme Techniques; D007422:Intestines / Q000032:analysis; D008297:Male; D010029:Ostertagiasis / Q000276:immunology / Q000662:veterinary*; D014252:Trichostrongyloidiasis / Q000662:veterinary*
chemical_list: D002630:Chemotactic Factors; D002631:Chemotactic Factors, Eosinophil