title: [Plasma hemoglobin. Comparison between 2 methods of determination].
abstract: The methods used to titrate plasmatic haemoglobin, an analysis which is of great interest to cardiac surgery, must be very accurate because the levels are much lower than those found in whole blood. This work introduces a comparison between two methods: the ammoniacal water method and Cripps's differential method. After studying various criteria (repeatability, limit of detection, accuracy, reference values, stability of the reaction), and after studying the correlation between each method and the benzidin method, used as reference method, as well as the correlation between all of them, Cripp's method turns out to be the closest to the benzidin method, as well as being simpler, more accurate, more specific, and also easier to use than the ammoniacal water method, the only problem occurring at the stage of the preparation of the standard solution.
authors.affiliation: Laboratoire d'Hématologie, Centre Hospitalier Régional, Caen, France.
authors.forename: M C
authors.initials: MC
authors.lastname: Khayat
authors.forename: F
authors.initials: F
authors.lastname: Laforet
authors.forename: F
authors.initials: F
authors.lastname: Bureau
authors.forename: M
authors.initials: M
authors.lastname: Thomas
authors.forename: M
authors.initials: M
authors.lastname: Drosdowsky
mesh_terms: D000641:Ammonia; D001560:Benzidines; D006452:Hemoglobinometry / Q000379:methods; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D011786:Quality Control; D013056:Spectrophotometry, Ultraviolet; D014867:Water
chemical_list: D001560:Benzidines; D006454:Hemoglobins; D014867:Water; C029876:benzidine; D000641:Ammonia
vernacular_title: Hémoglobine plasmatique. Comparison entre deux méthodes de dosage.