Endothelin immunoreactivity in medium from cultured porcine aortic endothelial cells correlates with the biological activity.
Using the radioimmunoassay (RIA) of endothelin (ET), we measured immunoreactive ET (IR-ET) in culture medium of porcine aortic endothelial cells. The immunoreactivity in the medium was compared with the biological activity. The amount of IR-ET released into the medium was calculated at 250-350 pg/10(6) cells/hr. The amount of IR-ET released into the culture medium increased progressively with 3-24 hr of incubation and corresponded to the increase in medium-induced vasoconstriction of rat isolated aorta. When the vasoconstrictor activities in the culture medium were plotted against the IR-ET concentration determined by RIA, the concentration-response curve showed similarity to that obtained with synthetic porcine ET. This RIA system will be a useful for investigating mechanisms of ET secretion from endothelial cells.
Matsumura
Y
Y
Department of Pharmacology, Osaka University of Pharmaceutical Sciences, Japan.
Ikegawa
R
R
Ohyama
T
T
Hayashi
K
K
Morimoto
S
S
D000818:Animals; D001013:Aorta, Thoracic; D002478:Cells, Cultured; D003470:Culture Media; D016232:Endothelins; D004730:Endothelium, Vascular / Q000032:analysis* / Q000502:physiology; D008297:Male; D009119:Muscle Contraction; D010455:Peptides / Q000032:analysis* / Q000502:physiology / Q000592:standards; D011863:Radioimmunoassay / Q000379:methods / Q000592:standards; D051381:Rats; D011919:Rats, Inbred Strains; D013552:Swine; D014662:Vasoconstrictor Agents / Q000032:analysis
D003470:Culture Media; D016232:Endothelins; D010455:Peptides; D014662:Vasoconstrictor Agents