title: Immunoassay based on surface plasmon oscillations.
abstract: An in situ immunoassay technique is described which is sensitive to specific binding to surface immobile antigen or antibody. The phenomena of surface plasmon oscillations are shown to be sensitive to antigen-antibody binding on a substrate. An advantage of the technique is that no molecular labeling is required. The technique allows the detection of the presence of approximately 6 X 10(8) molecules on the approximately 1 mm2 area of detection (approximately 1.5 X 10(-8) g/cm2). An elementary apparatus is described which allows the kinetics of the antigen-antibody binding to be accurately recorded in real time. A detailed example is presented. A second version of the apparatus of similar accuracy with improved temporal resolution is described.
authors.affiliation: Department of Physics and Astronomy, University of Massachusetts, Amherst 01003.
authors.forename: C S
authors.initials: CS
authors.lastname: Mayo
authors.forename: R B
authors.initials: RB
authors.lastname: Hallock
mesh_terms: D000327:Adsorption; D000937:Antigen-Antibody Reactions; D007163:Immunosorbent Techniques / Q000295:instrumentation*; D007700:Kinetics; D012834:Silver; D013499:Surface Properties
chemical_list: D012834:Silver