Activation of oncogenes in human oral cancer cells: a novel codon 13 mutation of c-H-ras-1 and concurrent amplifications of c-erbB-1 and c-myc.
By NIH3T3 transfection assay in conjunction with in vitro transient neomycin selection, activated c-H-ras-1 oncogenes were detected in two squamous cell carcinoma cell lines, ZA and HOC-313, newly established from human oral cancer patients. ZA had a point mutational activation at the 13th codon, this activation of c-H-ras-1 being novel in human cancer cells, while HOC-313 appeared to have an activation at the 12th codon. In ZA, 16- to 32-fold amplification of the EGF receptor gene, c-erbB-1 and a few-fold amplification of c-myc were detected. The significance of these findings is discussed in relation to multistep carcinogenesis in human cells.
Tadokoro
K
K
Department of Oral Microbiology, Faculty of Dentistry, Tokyo Medical and Dental University, Japan.
Ueda
M
M
Ohshima
T
T
Fujita
K
K
Rikimaru
K
K
Takahashi
N
N
Enomoto
S
S
Tsuchida
N
N
D000368:Aged; D001483:Base Sequence; D003062:Codon; D005091:Exons; D005784:Gene Amplification; D011905:Genes, ras; D006801:Humans; D008297:Male; D009062:Mouth Neoplasms / Q000235:genetics*; D009154:Mutation; D011519:Proto-Oncogenes; D012333:RNA, Messenger; D014162:Transfection
D003062:Codon; D012333:RNA, Messenger