[Chylomicron metabolism in experimental cholestasis].
Cholesterol and triglyceride plasma levels are usually increased in cholestasis. The excess of cholesterol and that of triglyceride are carried in abnormal low-density lipoproteins (LP) named LP-X and beta 2-LP respectively. It has been assumed that chylomicron metabolism is involved in these alterations. To gain insight into the LP disturbances in this pathology, artificial chylomicrons (AC) were prepared in protein-free aqueous solutions containing lecithin, cholesteryl-oleate, cholesterol and triolein. AC were labelled simultaneously with cholesteryl--14C-oleate (14C-CO) and 3H-triolein (3H-TO) and pulse injected intra-arterially in rats subjected to total obstruction of the bile duct for 48 hours and sham-operated rats. Blood was collected at 2-minute intervals during 10 minutes for radioactivity determination. Fractional clearance rates of 3H-TO and 14C-CO were diminished. Since plasma decay of 3H-TO reflects predominantly the rate of lipolysis (L) whereas chylomicron remnant removal (CRR) by the liver is represented by the 14C-CO decay, our data suggest that in cholestasis both L and CRR are defective.
Damião
A O
AO
Sipahi
A M
AM
Quintão
E C
EC
D000818:Animals; D001651:Cholestasis, Extrahepatic / Q000378:metabolism*; D002784:Cholesterol / Q000378:metabolism*; D002914:Chylomicrons / Q000378:metabolism*; D008073:Lipoprotein-X / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014280:Triglycerides / Q000378:metabolism*
D002914:Chylomicrons; D008073:Lipoprotein-X; D014280:Triglycerides; D002784:Cholesterol
Metabolismo de quilomícros na colestase experimental.