title: Low cost apparatus for primer-directed DNA amplification using Thermus aquaticus-DNA polymerase.
abstract: An apparatus is described which permits the incubation of samples at three different temperatures in a cyclic fashion. The parts for the incubator are either present in every biochemical laboratory (water baths) or can be easily obtained at a low price (timers and magnetic valves). Thus the new DNA amplification procedure employing Thermus aquaticus-DNA polymerase can be carried out automatically without major investments.
authors.affiliation: Institut fuer Biochemie, Vienna, Austria.
authors.forename: H
authors.initials: H
authors.lastname: Torgersen
authors.forename: D
authors.initials: D
authors.lastname: Blaas
authors.forename: T
authors.initials: T
authors.lastname: Skern
mesh_terms: D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D005784:Gene Amplification; D005821:Genetic Techniques / Q000295:instrumentation; D007700:Kinetics; D019914:Taq Polymerase; D013816:Thermodynamics; D013824:Thermus / Q000201:enzymology*
chemical_list: D019914:Taq Polymerase; D004259:DNA-Directed DNA Polymerase