title: Transforming growth factor-beta stimulates the expression of endothelin mRNA by vascular endothelial cells.
abstract: Endothelin is a potent vasoconstrictor peptide produced by vascular endothelial cells. Incubation of the serum-deprived confluent porcine aortic endothelial cells with 10-300 pM TGF-beta 1, resulted in a several fold increase in endothelin mRNA levels with a peak time of 2 h. An enzyme-linked immunosorbent assay revealed that the levels of endothelin in endothelial cell conditioned media was also increased by TGF-beta 1. These results suggest that TGF-beta 1, secreted by activated platelets, is involved not only in wound healing, but in the regulation of local vascular tone by stimulating endothelin production in the endothelial cells.
authors.affiliation: Third Department of Internal Medicine, University of Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kurihara
authors.forename: M
authors.initials: M
authors.lastname: Yoshizumi
authors.forename: T
authors.initials: T
authors.lastname: Sugiyama
authors.forename: F
authors.initials: F
authors.lastname: Takaku
authors.forename: M
authors.initials: M
authors.lastname: Yanagisawa
authors.forename: T
authors.initials: T
authors.lastname: Masaki
authors.forename: M
authors.initials: M
authors.lastname: Hamaoki
authors.forename: H
authors.initials: H
authors.lastname: Kato
authors.forename: Y
authors.initials: Y
authors.lastname: Yazaki
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic; D002478:Cells, Cultured; D016232:Endothelins; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D007700:Kinetics; D010455:Peptides / Q000235:genetics*; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics*; D013552:Swine; D014158:Transcription, Genetic / Q000187:drug effects*; D015291:Transforming Growth Factors / Q000494:pharmacology*
chemical_list: D016232:Endothelins; D010455:Peptides; D012333:RNA, Messenger; D015291:Transforming Growth Factors