title: Introduction and expression of the human insulin gene in adult rat liver.
abstract: Liposomes associated with gangliosides and loaded with the human insulin gene were incubated with HVJ (Sendai virus) and red blood cell membranes containing non-histone chromosomal protein, high mobility group 1 (HMG-1), or bovine serum albumin (BSA) to form vesicle complexes. These vesicle complexes were then injected into the portal vein of adult rats. The insulin gene co-introduced with HMG-1 was transported into the nuclei of liver cells much more efficiently than the gene co-introduced with BSA. Moreover, the amount of transcript of the insulin gene co-introduced with HMG-1 was more than 10 times greater than that of the gene co-introduced with BSA. Similar amounts of DNA and RNA of human insulin were found in the liver within 7 days after injection of the gene, and then the amounts of both decreased rapidly. The level of human insulin in rat serum gradually increased to a maximum (20-30 microunits/ml) after 7 or 8 days and then decreased rapidly.
authors.affiliation: Institute for Molecular and Cellular Biology, Osaka University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kaneda
authors.forename: K
authors.initials: K
authors.lastname: Iwai
authors.forename: T
authors.initials: T
authors.lastname: Uchida
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D002467:Cell Nucleus / Q000378:metabolism; D004247:DNA / Q000008:administration & dosage; D004337:Drug Carriers; D005796:Genes; D006801:Humans; D007328:Insulin / Q000096:biosynthesis / Q000097:blood / Q000235:genetics*; D007700:Kinetics; D008081:Liposomes; D008099:Liver / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D051381:Rats
chemical_list: D004337:Drug Carriers; D007328:Insulin; D008081:Liposomes; D004247:DNA