Subunit composition of photosystem I and identification of center X as a [4Fe-4S] iron-sulfur cluster.
A photosystem I (PS-I) preparation from barley (Hordeum vulgare L.) containing the reaction center protein P700-chlorophyll a-protein 1 (CP1) and smaller polypeptides with apparent molecular masses of 18, 16, 14, 9.5, 9, 4, and 1.5 kDa has been analyzed with respect to subunit stoichiometry. CP1 contains two homologous subunits with approximate masses of 82 kDa. CP1 and the smaller polypeptides were isolated, and the amino acid composition of each component and of the PS-I preparation was determined. Based on the amino acid composition data and the determined ability of each isolated polypeptide to bind Coomassie Brilliant Blue, the PS-I complex is shown to contain 1 mol of each of the homologous 82-kDa polypeptides as well as 1 mol of the 18-, 16-, 9.5-, and 9-kDa polypeptides for each mol of P700. The total polypeptide mass of the PS-I complex is 209 kDa excluding tryptophan and approximately 220 kDa including tryptophan. The two 82-kDa subunits present/P700 provide cysteine residues for binding only one Fe-S center. In conjunction with the earlier reported binding of four iron and four acid-labile sulfides to CP1/P700 (Høj, P. B., Svendsen, I., Scheller, H. V., and Møller, B. L. (1987) J. Biol. Chem. 262, 12676-12684), this demonstrates the center X is a [4Fe-4S] cluster and eliminates the possibility of center X being composed of two [2Fe-2S] clusters.
Scheller
H V
HV
Department of Plant Physiology, Royal Veterinary and Agricultural University, Valby, Denmark.
Svendsen
I
I
Møller
B L
BL
D000596:Amino Acids / Q000032:analysis; D002734:Chlorophyll / Q000032:analysis*; D002736:Chloroplasts / Q000502:physiology*; D007506:Iron-Sulfur Proteins / Q000032:analysis*; D045342:Light-Harvesting Protein Complexes; D046911:Macromolecular Substances; D008667:Metalloproteins / Q000032:analysis*; D008970:Molecular Weight; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D010940:Plant Proteins / Q000032:analysis*; D010944:Plants; D011485:Protein Binding; D012394:Rosaniline Dyes / Q000378:metabolism
D000596:Amino Acids; D007506:Iron-Sulfur Proteins; D045342:Light-Harvesting Protein Complexes; D046911:Macromolecular Substances; D008667:Metalloproteins; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D010940:Plant Proteins; D012394:Rosaniline Dyes; D002734:Chlorophyll; C024757:Coomassie brilliant blue R