Comparative studies of in vitro transformation by ethylene oxide and gamma-radiation of C3H/10T1/2 cells.
A dose-dependent transforming ability of the direct-acting alkylating agent, ethylene oxide (EtO), was demonstrated in C3H/10T1/2 mouse embryo fibroblasts. Morphologically transformed colonies were observed 5-6 weeks after the treatment with EtO. The transforming effectiveness of EtO was compared with that of gamma-radiation, and the rad-equivalence of EtO was calculated to be approximately 90 rad/mMh. This value is consistent with earlier estimates based on induced mutation in various biological systems and thus indicates the applicability of the C3H/10T1/2 cell system in the risk estimation of human exposure to genotoxic compounds.
Kolman
A
A
Department of Radiobiology, University of Stockholm, Sweden.
Näslund
M
M
Osterman-Golkar
S
S
Scalia-Tomba
G P
GP
Meyer
A
A
D000818:Animals; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D004622:Embryo, Mammalian; D005027:Ethylene Oxide / Q000008:administration & dosage / Q000633:toxicity*; D005347:Fibroblasts / Q000187:drug effects / Q000528:radiation effects; D005720:Gamma Rays; D051379:Mice; D008815:Mice, Inbred Strains; D009024:Morphogenesis / Q000187:drug effects
D005027:Ethylene Oxide