Detection of a Myc-associated protein by chemical cross-linking.
A single nuclear protein (Myc-associated protein) can be specifically cross-linked to avian Myc proteins by treatment of nuclei or cells with the reversible cross-linker dimethyl 3,3'-dithiobis-propionimidate. Myc-associated protein has a molecular weight of approximately 500,000, is not detectably phosphorylated and, in contrast to Myc, has a long apparent half-life of greater than 3 h.
Gillespie
D A
DA
Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
Eisenman
R N
RN
D000818:Animals; D002461:Cell Line, Transformed; D003432:Cross-Linking Reagents; D006207:Half-Life; D007096:Imidoesters; D008970:Molecular Weight; D011518:Proto-Oncogene Proteins / Q000302:isolation & purification* / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc
D003432:Cross-Linking Reagents; D007096:Imidoesters; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; C007471:dimethyl dithiobispropionimidate