title: Centromere proteins. I. Mitosis specific centromere antigen recognized by anti-centromere autoantibodies.
abstract: Human anti-centromere sera from scleroderma patients were used to detect centromere antigens of mouse fibroblast cells. An Mr = 59,000 centromere protein was localized exclusively on mitotic chromosomes. The association of this protein with the mitotic chromosomes proved to be DNase I sensitive. In interphase nuclei, this centromere antigen was not detectable by immunoblot techniques. The results suggest that the Mr = 59,000 mitosis specific protein may be necessary for the structural stability of kinetochores during mitosis.
authors.affiliation: Institute of Genetics, Hungarian Academy of Sciences, Szeged.
authors.forename: G
authors.initials: G
authors.lastname: Hadlaczky
authors.forename: T
authors.initials: T
authors.lastname: Praznovszky
authors.forename: I
authors.initials: I
authors.lastname: Rasko
authors.forename: J
authors.initials: J
authors.lastname: Kereso
mesh_terms: D000818:Animals; D001323:Autoantibodies / Q000276:immunology*; D002478:Cells, Cultured; D002503:Centromere / Q000276:immunology*; D002875:Chromosomes / Q000032:analysis / Q000276:immunology*; D003850:Deoxyribonuclease I / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts; D005455:Fluorescent Antibody Technique; D006801:Humans; D015151:Immunoblotting; D051379:Mice; D008938:Mitosis; D011233:Precipitin Tests; D012259:Ribonuclease, Pancreatic / Q000378:metabolism
chemical_list: D001323:Autoantibodies; D003850:Deoxyribonuclease I; D012259:Ribonuclease, Pancreatic