Transformation of growth factor-dependent myeloid stem cells with retroviral vectors carrying c-myc.
Myeloid progenitor cells and macrophages derived from bone marrow and spleen were efficiently transformed in vitro by infection with Moloney-based retroviral vectors carrying a human c-myc gene. Infected cells were plated in agar in the presence of combinations of the murine lymphokines CSF-1, IL-3, GM-CSF and IL-1. Between 20% and 100% of the colony-forming cells in the initial bone marrow or spleen population could be infected and gave rise to drug-resistant colonies. A large fraction of the infected cells showed continued proliferation after transfer to liquid media and we have derived over 200 growth factor-dependent cell lines. These include adherent and non-adherent CSF-1 or GM-CSF dependent macrophages and macrophage precursors and cell lines which require complex combinations of growth factors for optimal growth. Each of the cell lines displays a unique pattern of expression of surface markers specific for the myeloid lineage including the Mac-1, Mac-2, Mac-3, Ser-4 and F4/80 antigens. Surface markers not specifically associated with the myeloid lineage such as the MHC class II antigens and the Fc-receptor; and surface markers normally associated with the B-cell and T-cell lineages such as B220, L3T4 and Thy1.2 are also found on these cell lines.
Green
S M
SM
MRC Laboratory of Molecular Biology, Cambridge, UK.
Lowe
A D
AD
Parrington
J
J
Karn
J
J
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology; D015139:Blotting, Southern; D001854:Bone Marrow Cells; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000473:pathology; D002472:Cell Transformation, Viral; D002999:Clone Cells / Q000145:classification / Q000166:cytology / Q000276:immunology; D003115:Colony-Stimulating Factors / Q000096:biosynthesis; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D005822:Genetic Vectors; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000096:biosynthesis / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000382:microbiology; D006801:Humans; D007377:Interleukin-3 / Q000096:biosynthesis; D008264:Macrophages / Q000166:cytology / Q000276:immunology / Q000382:microbiology; D051379:Mice; D011519:Proto-Oncogenes; D011533:Proviruses / Q000235:genetics / Q000502:physiology; D012190:Retroviridae / Q000235:genetics* / Q000502:physiology; D013154:Spleen / Q000166:cytology
D000954:Antigens, Surface; D003115:Colony-Stimulating Factors; D004279:DNA, Viral; D006133:Growth Substances; D007377:Interleukin-3; D016178:Granulocyte-Macrophage Colony-Stimulating Factor