Role of the phenylalanine B24 side chain in directing insulin interaction with its receptor. Importance of main chain conformation.
We have investigated (by use of semisynthetic insulin analogs and isolated canine hepatocytes) the role of invariant residue PheB24 in determining the affinity of insulin-receptor interactions. Our results confirm that replacement of PheB24 by D-Phe is not detrimental to ligand binding to receptor, show that D-Ala is well tolerated at position B24 (whereas Ala is not), and demonstrate that [GlyB24]insulin retains as much as 78% of the receptor binding potency of native insulin. Additional findings show that replacement of PheB24 by D-Pro or by alpha-aminoisobutyric acid results in analogs with severely decreased binding potency, and that the COOH-terminal domain containing residues B26-B30 plays a positive role in determining receptor binding potency in GlyB24-substituted insulin (whereas it plays a negative role in determining the receptor binding potency of its GlyB25-substituted counterpart). We interpret our results as identifying (a) a critical role for the insulin main chain near residue B24 in determining the affinity of receptor for ligand, (b) the importance of main chain flexibility in achieving a high affinity state of receptor-bound hormone, and (c) a potential interaction of the PheB24 side chain with receptor which initiates main chain structural changes in the natural hormone, but which does not itself confer affinity to ligand-receptor interactions.
Mirmira
R G
RG
Department of Biochemistry and Molecular Biology, University of Chicago, Illinois 60637.
Tager
H S
HS
D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D066298:In Vitro Techniques; D007328:Insulin / Q000138:chemical synthesis / Q000502:physiology*; D008099:Liver / Q000378:metabolism; D010649:Phenylalanine; D011487:Protein Conformation; D011972:Receptor, Insulin / Q000378:metabolism*; D013329:Structure-Activity Relationship
D007328:Insulin; D010649:Phenylalanine; D011972:Receptor, Insulin