Determination of terbutaline enantiomers in biological samples using liquid chromatography with coupled columns.
The purpose of this work was to develop and validate a method for the separation and determination of the enantiomers of terbutaline in plasma and intestinal juice. Terbutaline was extracted from plasma and intestinal juice by liquid-solid extraction on small C18 cartridges. The extract was then analyzed by coupled column liquid chromatography with amperometric detection. For chiral separation a beta-cyclodextrin phase was used. The within-day variation (Cv) on spiked plasma samples was in the range 0.8-6.4% at 3.8-33.8 nmol/liter for the (-)-enantiomer, and 2.6-23.0% at 1.3-11.3 nmol/liter for the (+)-enantiomer. The between-day variation on spiked plasma samples was 5.5% at 10.7 nmol/liter and 13.6% at 4.3 nmol/liter for the (-)-and (+)-enantiomers, respectively. The within-day variation for intestinal juice was in the range 0.7-1.5% at 5.6-30.0 mumol/liter for the (+)-enantiomer.
Walhagen
A
A
Chemical Centre, University of Lund, Sweden.
Edholm
L E
LE
Kennedy
B M
BM
Xiao
L C
LC
D001826:Body Fluids / Q000032:analysis; D002021:Buffers; D002853:Chromatography, Liquid; D003505:Cyclodextrins; D004563:Electrochemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007422:Intestines / Q000032:analysis; D009961:Orosomucoid; D013237:Stereoisomerism; D013696:Temperature; D013726:Terbutaline / Q000032:analysis* / Q000097:blood; D047392:beta-Cyclodextrins
D002021:Buffers; D003505:Cyclodextrins; D009961:Orosomucoid; D047392:beta-Cyclodextrins; C031215:betadex; D013726:Terbutaline