title: The role of glucose and glycosylation in the regulation of lipoprotein lipase synthesis and secretion in rat adipocytes.
abstract: Several studies have suggested that insulin and glucose increase adipose tissue lipoprotein lipase (LPL). To study the mechanism of the glucose-induced stimulation of LPL, the effects of glucose and glycosylation were examined in primary rat adipocyte cultures. In cells cultured in the presence of 1 mg/ml glucose, a 55-kDa LPL protein was synthesized and secreted into the medium, whereas cells cultured in glucose-free medium synthesized a 49-kDa form of LPL which was not secreted. The treatment of the mature 55-kDa form of LPL with peptide:N-glycosidase-F resulted in the formation of a 49-kDa form of LPL. When cells were cultured in the presence of tunicamycin, a 49-kDa form of LPL was synthesized by the cells but was not secreted. In addition, LPL activity was reduced by 90% when glycosylation was blocked by either tunicamycin or glucose deprivation. LPL synthetic rate was examined in cells cultured in a spectrum of glucose concentrations. LPL synthetic rate increased directly with medium glucose concentration and was decreased 80% in the absence of glucose compared to the synthetic rate in the presence of 1 mg/ml glucose. In addition, LPL synthetic rate in the presence of insulin was approximately 200% of the synthetic rate in untreated control cells at all glucose concentrations and even in the absence of glucose. In spite of the effect of glucose on LPL synthetic rate, glucose had no effect on the level of LPL mRNA. In contrast, the mRNA for the 78-kDa glucose-regulated protein (GRP78) was increased in adipocytes cultured in glucose-free medium. In summary, glucose was essential for glycosylation of LPL, and glycosylation was essential for LPL catalytic activity and secretion. In addition, glucose stimulated LPL synthetic rate and potentiated the stimulatory effects of insulin, but had no specific effect on LPL mRNA. Whereas insulin stimulates LPL by increasing the level of LPL mRNA, glucose stimulates LPL translation and post-translational processing.
authors.affiliation: Department of Medicine, Cedars-Sinai Medical Center, Los Angeles, California 90048.
authors.forename: J M
authors.initials: JM
authors.lastname: Ong
authors.forename: P A
authors.initials: PA
authors.lastname: Kern
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D015342:DNA Probes; D004591:Electrophoresis, Polyacrylamide Gel; D005947:Glucose / Q000494:pharmacology*; D006031:Glycosylation; D007163:Immunosorbent Techniques; D007328:Insulin / Q000494:pharmacology; D007700:Kinetics; D008071:Lipoprotein Lipase / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D008970:Molecular Weight; D014176:Protein Biosynthesis / Q000187:drug effects; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014415:Tunicamycin / Q000494:pharmacology
chemical_list: D015342:DNA Probes; D007328:Insulin; D012333:RNA, Messenger; D014415:Tunicamycin; D008071:Lipoprotein Lipase; D005947:Glucose