Sensitivity and specificity of an enzyme-linked immunosorbent assay using the recombinant DNA-derived Treponema pallidum protein TmpA for serodiagnosis of syphilis and the potential use of TmpA for assessing the effect of antibiotic therapy.
The recombinant DNA-derived Treponema pallidum membrane protein TmpA, purified from Escherichia coli K-12, was used in an enzyme-linked immunosorbent assay (ELISA) to evaluate its suitability in a screening test for syphilis and to monitor the effect of antibiotic treatment. The sensitivity of the TmpA ELISA was 76% for primary syphilis, 100% for secondary syphilis, and 98% for early latent syphilis. All except 1 of 15 serum samples positive for yaws were positive in this test. A specificity of 99.6% was found by testing more than 938 donor samples. The sensitivity and specificity of the TmpA ELISA are comparable to that of the T. pallidum hemagglutination assay, and therefore the test may be useful for the diagnosis of untreated syphilis. After antibiotic treatment, the level of anti-TmpA antibodies in sera of syphilis patients dropped sharply within 1 year. Thus, TmpA might be a useful antigen for monitoring successful treatment of syphilis.
Ijsselmuiden
O E
OE
Department of Dermatovenereology, University Hospital Dijkzigt, Rotterdam, The Netherlands.
Schouls
L M
LM
Stolz
E
E
Aelbers
G N
GN
Agterberg
C M
CM
Top
J
J
van Embden
J D
JD
D000208:Acute Disease; D000907:Antibodies, Bacterial / Q000032:analysis; D000942:Antigens, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology*; D002908:Chronic Disease; D004274:DNA, Recombinant; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006386:Hemagglutination Tests; D006801:Humans; D007700:Kinetics; D008565:Membrane Proteins / Q000276:immunology; D011237:Predictive Value of Tests; D011994:Recombinant Proteins / Q000276:immunology; D013587:Syphilis / Q000188:drug therapy / Q000276:immunology; D013588:Syphilis Serodiagnosis; D014210:Treponema pallidum / Q000235:genetics / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004274:DNA, Recombinant; D008565:Membrane Proteins; D011994:Recombinant Proteins