Compartmentation of deoxypyrimidine nucleotides for nuclear DNA replication in S phase mammalian cells.
DNA synthesis in S phase Chinese hamster embryo fibroblast cells in the presence of exogenous 3H-dUrd shows incorporation of the labeled precursor with very little dilution by the large unlabeled intracellular precursor pools. Full mixing would predict a specific activity 10-fold less than that measured. This coupled with the finding that 80% of the radioactivity derived from the exogenous 3H-dUrd appears in the karyoplasts implies a compartmentation where 3H-dUMP and 3H-dTTP derived from exogenous 3H-dUrd do not mix freely with endogenous cytoplasmic pools.
Reddy
G P
GP
Department of Obstetrics and Gynaecology, University of Virginia, Charlottesville 22908.
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D004247:DNA / Q000378:metabolism; D004261:DNA Replication; D003856:Deoxyuracil Nucleotides / Q000378:metabolism*; D003857:Deoxyuridine / Q000378:metabolism*; D007399:Interphase; D007700:Kinetics; D013942:Thymine Nucleotides / Q000378:metabolism*
D003856:Deoxyuracil Nucleotides; D013942:Thymine Nucleotides; D004247:DNA; C007267:2'-deoxyuridylic acid; C024157:thymidine 5'-triphosphate; D003857:Deoxyuridine