Morphology of interactions of the haematopoietic microenvironment with the haematopoietic cells in erythroid spleen colonies.
The morphology of the interactions of the cells of the haematopoietic microenvironment with haematopoietic cells was studied in exogenous erythroid spleen colonies formed in mice, on the fifth and eighth day after their irradiation with a lethal dose of gamma rays and bone marrow transplantation. The characteristic type of stromal cell interacting with less mature cells of the erythroid series was a dark, branching reticular cell. The typical structural interaction of the reticular cells with erythroblasts was the formation of very long, fine cytoplasmic processes by the reticular cells. The processes were in close contact with the erythroblasts and formed a three-dimensional network stretching long distances from the nucleus of the reticular cells. Other cells of the haematopoietic microenvironment, in contact chiefly with poly- and ortochromic erythroblasts, were macrophages and the two together formed typical erythroblastic islands. In places, the macrophages and erythroblasts formed close, firm contacts by means of their cytoplasmic membranes. These morphological observations support the conception that close functional cooperation exists between the cells of the haematopoietic microenvironment and the haematopoietic cells in differentiation and proliferation processes in the haematopoietic tissue of spleen colonies.
Hofer
M
M
Tkadlecek
L
L
Viklická
S
S
Karpfel
Z
Z
D000818:Animals; D002450:Cell Communication / Q000502:physiology*; D015672:Erythroid Precursor Cells / Q000166:cytology*; D005260:Female; D006410:Hematopoiesis / Q000502:physiology*; D051379:Mice; D013154:Spleen / Q000166:cytology* / Q000648:ultrastructure