The beneficial effect of superoxide dismutase on the rat liver graft.
We performed orthotopic liver transplantation in male Wistar rats and investigated the effect of superoxide dismutase (SOD) on the liver graft. Animals were divided into the four following experimental groups. Group I was an untreated control group, group II received oxygen, group III received SOD and group IV received both oxygen and SOD. The dose of SOD was 3 mg/kg which was injected intravenously into both donors and recipients during the operation. Oxygen was given through an oxygen inhaler to both donors and recipients during the operation. The preservation time of the liver graft ranged from 4 hours and 41 minutes to 5 hours and 40 minutes. The survival after liver transplantation was compared among groups I, II, III and IV. Group IV showed a significantly higher survival rate than groups I and II by two weeks after liver grafting, but there was no statistical difference in the survival rates between groups III and IV. These results indicate the beneficial effect of SOD on the rat liver graft and may implicate oxygen free radicals in the pathogenesis of ischemia/reperfusion injury in liver grafts.
Mizuta
T
T
Department of Surgery, Kanto Teishin Hospital, Tokyo, Japan.
Saito
A
A
Kawano
N
N
Nagao
T
T
Morioka
Y
Y
D000818:Animals; D005609:Free Radicals; D006085:Graft Survival / Q000187:drug effects*; D016031:Liver Transplantation; D008297:Male; D010100:Oxygen; D051381:Rats; D011919:Rats, Inbred Strains; D013482:Superoxide Dismutase / Q000494:pharmacology*
D005609:Free Radicals; D013482:Superoxide Dismutase; D010100:Oxygen