Identification of the sigma 1S protein in reovirus serotype 2-infected cells with antibody prepared against a bacterial fusion protein.
A bacterial expression vector, pATH 3, was used to produce high levels of a fusion protein composed of a portion of the trpE protein of Escherichia coli and the putative sigma 1S coding region from the S1 gene of reovirus serotype 2. The fusion protein was purified and injected into rabbits to prepare antisera. This antibody was able to detect sigma 1S being synthesized in L929 cells infected with reovirus serotype 2 by means of immunoprecipitation and immunoblotting techniques. The peak of sigma 1S accumulation in type 2-infected cells was shown to occur approximately 20 hr after infection. This report represents the first description of sigma 1S production in reovirus serotype 2-infected cells.
Cashdollar
L W
LW
Department of Microbiology, Medical College of Wisconsin, Milwaukee 53226.
Blair
P
P
Van Dyne
S
S
D000914:Antibodies, Viral / Q000276:immunology*; D036022:Capsid Proteins; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D007106:Immune Sera / Q000276:immunology; D015151:Immunoblotting; D011233:Precipitin Tests; D011993:Recombinant Fusion Proteins / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology*; D012087:Reoviridae / Q000378:metabolism*; D014764:Viral Proteins / Q000032:analysis* / Q000096:biosynthesis
D000914:Antibodies, Viral; D036022:Capsid Proteins; D007106:Immune Sera; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D014764:Viral Proteins; C031728:sigma 1 protein, reovirus