title: Vero cytotoxin production and HeLa cell adherence of enteropathogenic Escherichia coli of infantile diarrhoea in Iran.
abstract: A total of 70 enteropathogenic Escherichia coli (EPEC) strains belonging to 11 serogroups, isolated from infantile diarrhoea in Tehran, Iran, were tested for the production of verocytotoxin (VT), enterotoxin, and also for their adherence to HeLa cells. In total 55 (78.5%) strains were either VT (32 strains) or enterotoxin (23 strains) producers, and of these 8 strains produced both VT and enterotoxins. 57 (81.4%) strains showed either Localized (LA) or Diffuse adherence (DA) or both types of adhesion (LA/DA) on HeLa cells, with strains showing LA/DA in the same preparations being dominant (32 strains), followed by those showing LA (14 strains) and DA (11 strains). Among adherent EPEC, 26 (37.1%) strains belonging to the serogroups 020, 086, 0119, 0125, 0126, 0127 and 0128 also produced VT. These findings suggest that production of VT and enterotoxin is an important factor in the pathogenesis of EPEC diarrhoea in Iran and that the combination of adherence and production of toxins is a common feature of EPEC strains which cause diarrhoea in this country.
authors.affiliation: Department of Microbiology, Pasteur Institute of Iran, Tehran.
authors.forename: M
authors.initials: M
authors.lastname: Katouli
authors.forename: A
authors.initials: A
authors.lastname: Pachenary
authors.forename: G R
authors.initials: GR
authors.lastname: Ketabi
mesh_terms: D001422:Bacterial Adhesion; D001427:Bacterial Toxins / Q000096:biosynthesis*; D003603:Cytotoxins / Q000096:biosynthesis*; D003968:Diarrhea, Infantile / Q000382:microbiology*; D004926:Escherichia coli / Q000472:pathogenicity*; D006367:HeLa Cells; D006801:Humans; D022622:Shiga Toxin 1
chemical_list: D001427:Bacterial Toxins; D003603:Cytotoxins; D022622:Shiga Toxin 1