title: Effect of estrogen on platelet aggregation in guinea pigs.
abstract: This experiment was carried out to explore the effect of estrogen on platelet aggregation, using Hartly strain guinea pigs. For the in vivo experiments, estradiol benzoate 1 mg/kg or 4 mg/kg was injected into male guinea pigs to observe the effects of this drug on platelet aggregation. In vitro studies were performed by incubating platelet-rich plasma with estradiol benzoate for 30 minutes at 37 degrees C. Aggregation was induced by the addition of aggregating agents: adenosine diphosphate (ADP, lobster or rabbit muscle, Merck), and collagen (calf skin, Bio-Data). The change in transmittance at 660 nm was recorded using NKK HEMA TRACER 1, model PAT-4A (Nippon Denshi Kagaku Co., Japan). Our results revealed that a 1 mg/kg injection of estrogen had a statistically significant suppressive effect on both ADP and collagen-induced platelet aggregation. While in the group with the 4 mg/kg injection of estrogen, this suppressing effect was not significant. In vitro studies by incubating platelet-rich plasma with estradiol had no inhibitory effect on aggregation induced by both ADP and collagen.
authors.forename: R Y
authors.initials: RY
authors.lastname: Yuan
authors.forename: T K
authors.initials: TK
authors.lastname: Lee
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D003094:Collagen / Q000494:pharmacology; D004958:Estradiol / Q000031:analogs & derivatives* / Q000494:pharmacology; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology
chemical_list: D010975:Platelet Aggregation Inhibitors; D004958:Estradiol; D000244:Adenosine Diphosphate; D003094:Collagen; C010452:estradiol-17 beta-benzoate