[Enrichment of cytochrome P-450 in human liver microsomes by the action of proteases].
Human liver microsomes have been partially enriched in cytochrome P450 using a simple and rapid method. Microsomes were digested with protease XXVII (40 micrograms/nmole cyt. P450), then incubated with CHAPS, a zwitterionic detergent (25 mg/nmole cyt. P450) in combination with 0.07% protamine sulfate and the solubilized cyt. P450 was separated by ultracentrifugation. By this method, about 35% of total microsomal protein was solubilized with more than 80% of cyt. P450. This technique increases the specific activity of cyt. P450 by three fold.
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Cumps
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Fossoul
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Atassi
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D002793:Cholic Acids; D003577:Cytochrome P-450 Enzyme System / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D008862:Microsomes, Liver / Q000201:enzymology*; D010447:Peptide Hydrolases
D002793:Cholic Acids; D003577:Cytochrome P-450 Enzyme System; D010447:Peptide Hydrolases; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate
Enrichissement en cytochrome P450 de microsomes de foie humain par l'action de la protéase.