[Cloning of Pseudomonas putida genes responsible for the primary stages of oxidation of naphthalene in Escherichia coli cells].
Data on cloning Pseudomonas putida D-plasmid pBS286 (IncP-9) genes which are responsible for primary stages of naphthalene oxidation as well as data on the expression of these genes in Escherichia coli cells are presented. Recombinant plasmid pBS959 containing the whole constitutive nahA locus encoding naphthalene dioxygenase, a key enzyme of the naphthalene oxidation pathway, has been constructed on the basis of the pUC19 vector. An evidence has been obtained that at least a portion of the sequence of structural nahB gene is cloned in the recombinant pBS959 plasmid. Constitutive expression of the nahA gene is controlled by its own promoter and leads to conversion of indole to indigo in E. coli cells, strain HB101. Plasmid pBS959 is characterized by high structural stability; some instability observed is due to segregation.
Boronin
A M
AM
Tsoĭ
T V
TV
Kosheleva
I A
IA
Arinbasarov
M U
MU
Adanin
V M
VM
D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004396:Coloring Agents / Q000378:metabolism; D004247:DNA / Q000235:genetics; D049308:Dioxygenases; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D005822:Genetic Vectors; D007203:Indigo Carmine; D007211:Indoles / Q000378:metabolism; D009097:Multienzyme Complexes / Q000235:genetics; D009281:Naphthalenes / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D010084:Oxidation-Reduction; D010105:Oxygenases / Q000235:genetics; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011549:Pseudomonas / Q000235:genetics*
D004396:Coloring Agents; D007211:Indoles; D009097:Multienzyme Complexes; D009281:Naphthalenes; D004247:DNA; D007203:Indigo Carmine; D010105:Oxygenases; D049308:Dioxygenases; C038703:naphthalene dioxygenase
Klonirovanie genov pervichnykh étapov okisleniia naftalina Pseudomonas putida v kletkakh Escherichia coli.