A diabetic-like condition of turkey embryos maintained in shell-less culture.
Serum insulin concentration and pancreatic insulin content were determined for turkey embryos incubated in ovo and in long-term shell-less culture (ex ovo). Insulin was undetectable (less than 10 pg) in serum from 87% of the ex ovo embryos compared with their in ovo counterparts. This was evident at all incubation ages, although insulin was detectable in more of the ex ovo embryos on Day 24. Insulin increased in the embryos incubated in ovo from 122 (Day 15) to levels exceeding 2000 pg/ml at hatching. Total pancreatic insulin content was greater in the cultured embryos on Days 15, 17, and 22 compared with their in ovo counterparts. Serum glucose was significantly greater (P less than 0.05) in the ex ovo embryos at all ages. In response to an infusion of L-arginine, serum insulin increased from 566 to 1256 pg/ml in the in ovo embryos, whereas no change was evident in the ex ovo embryos (233 vs 257 pg/ml). When embryos incubated in ovo were injected with insulin, a significant (P less than 0.05) reduction of serum glucose was observed at 60 min after injection. Serum glucose concentrations remained elevated in the embryos incubated ex ovo despite the insulin injection. Liver glucose 6-phosphatase activity, assessed on Days 15 and 22 of incubation, was found to be significantly (P less than 0.05) lower in the ex ovo embryos. Turkey embryos incubated in shell-less culture exhibited chronic hyperglycemia in concert with extremely low circulating levels of insulin. The pancreatic beta cells of these embryos were not responsive to arginine or elevated glucose. Taken together these findings suggest the occurrence of a diabetic-like condition in the ex ovo embryos. This defect in insulin secretion may, in part, be responsible for some of the developmental abnormalities characteristic of the turkey embryo cultured ex ovo.
McMurtry
J P
JP
Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705.
Richards
M P
MP
Rosebrough
R W
RW
Steele
N C
NC
D000818:Animals; D001120:Arginine / Q000494:pharmacology; D001786:Blood Glucose / Q000032:analysis; D002908:Chronic Disease; D046508:Culture Techniques; D003920:Diabetes Mellitus / Q000097:blood / Q000503:physiopathology*; D004528:Egg Shell / Q000378:metabolism / Q000503:physiopathology*; D004625:Embryo, Nonmammalian / Q000378:metabolism / Q000503:physiopathology*; D006943:Hyperglycemia / Q000503:physiopathology; D007328:Insulin / Q000097:blood; D008099:Liver / Q000201:enzymology; D009929:Organ Size; D010179:Pancreas / Q000502:physiology; D014422:Turkeys
D001786:Blood Glucose; D007328:Insulin; D001120:Arginine