A 42K outer-membrane protein is a component of the yeast mitochondrial protein import site.
An engineered precursor protein that sticks in the import site of isolated yeast mitochondria can be specifically photo-crosslinked to a mitochondrial outer-membrane protein of relative molecular mass 42,000 (42K). This protein (termed import-site protein 42 or ISP 42) is exposed on the mitochondrial surface; antibodies against it block protein import into mitochondria. ISP 42 is the first identified component of the putative transmembrane machinery that imports proteins into mitochondria.
Vestweber
D
D
Biocenter, University of Basel, Switzerland.
Brunner
J
J
Baker
A
A
Schatz
G
G
D001692:Biological Transport; D003432:Cross-Linking Reagents; D005656:Fungal Proteins / Q000276:immunology / Q000502:physiology*; D007425:Intracellular Membranes / Q000502:physiology / Q000648:ultrastructure; D008928:Mitochondria / Q000502:physiology* / Q000648:ultrastructure; D008970:Molecular Weight; D010777:Photochemistry; D011233:Precipitin Tests; D011498:Protein Precursors / Q000378:metabolism; D012441:Saccharomyces cerevisiae
D003432:Cross-Linking Reagents; D005656:Fungal Proteins; D011498:Protein Precursors