Long-term cultured human vascular endothelial cells (EC-FP5) bind Plasmodium falciparum infected erythrocytes.
We have established an endothelial cell line, EC-FP5, that binds Plasmodium falciparum infected erythrocytes after several passages in culture and multiple cryopreservations. Binding by the EC-FP5 cells, measured as the percentage that bound infected erythrocytes and the average number of infected erythrocytes bound per endothelial cell, was similar in cells cryopreserved 1, 2, or 3 times. The parasite strain of the infected erythrocytes and culture conditions, including parasitemia and pH of the erythrocyte suspension, significantly affected binding. The capability of the EC-FP5 cells to be cultured in large amounts and cryopreserved in several aliquots will provide flexibility, reduce experimental variation, and enhance the utility of the endothelial cell-dependent cytoadherence assay.
Udeinya
I J
IJ
Walter Reed Army Institute of Research, Washington, DC.
Magowan
C
C
Chulay
J D
JD
D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D015925:Cryopreservation; D004727:Endothelium / Q000503:physiopathology*; D004912:Erythrocytes / Q000469:parasitology / Q000502:physiology*; D005312:Fetal Blood / Q000469:parasitology; D006801:Humans; D010963:Plasmodium falciparum