Effect of age on the binding of lectin 125I-PHA-B to pancreatic islets of rat in vitro and stimulation of some cellular events.
Agaricus bisporus lectin (PHA-B) stimulates insulin release in vitro. The stimulation is associated with increased conversion of proinsulin to insulin in the isolated islets of Langerhans of rats. Both these functions are directly proportional to the binding of I125 PHA-B, which is more marked in the islets from younger rats. The lectin binding to islets is not affected by glucose concentration in the medium.
Khalid
P
P
Division of Biochemistry, Central Drug Research Institute, Lucknow, India.
Ahmad
F
F
Khan
M M
MM
Rastogi
A K
AK
Kidwai
J R
JR
D000364:Agaricus; D000375:Aging / Q000378:metabolism*; D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism; D002458:Cell Fractionation; D002478:Cells, Cultured; D002850:Chromatography, Gel; D005947:Glucose / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism* / Q000494:pharmacology; D008297:Male; D011384:Proinsulin / Q000378:metabolism; D011863:Radioimmunoassay; D051381:Rats; D014357:Trypsin / Q000494:pharmacology
C041433:Agaricus lectins; D007328:Insulin; D037102:Lectins; D011384:Proinsulin; D014357:Trypsin; D005947:Glucose