title: Crystallization of the DNA-binding Escherichia coli protein FIS.
abstract: The specific DNA-binding protein FIS (factor for inversion stimulation), which stimulates site-specific DNA inversion by interaction with an enhancer sequence, was purified from an Escherichia coli strain overproducing the protein. FIS was crystallized at room temperature by microdialysis against 1.2 to 1.5 M-sodium/potassium phosphate containing 10 mM-Tris.HCl, 0.5 to 1 M-NaCl and 1 mM-NaN3 at pH 8.0 to 8.2. The crystals are stout prisms and suitable for X-ray diffraction study beyond 2.5 A resolution. They belong to the orthorhombic space group P2(1)2(1)2(1). The unit cell has dimensions a = 47.57(4) A, b = 51.13(4) A, c = 79.83(6) A and contains one FIS dimer in the asymmetric unit.
authors.affiliation: Institute für Kristallographie, Freie Universität Berlin, F.R.G.
authors.forename: H W
authors.initials: HW
authors.lastname: Choe
authors.forename: J
authors.initials: J
authors.lastname: Labahn
authors.forename: S
authors.initials: S
authors.lastname: Itoh
authors.forename: C
authors.initials: C
authors.lastname: Koch
authors.forename: R
authors.initials: R
authors.lastname: Kahmann
authors.forename: W
authors.initials: W
authors.lastname: Saenger
mesh_terms: D001426:Bacterial Proteins; D002352:Carrier Proteins; D003460:Crystallization; D004268:DNA-Binding Proteins; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D035021:Factor For Inversion Stimulation Protein; D034982:Integration Host Factors; D014961:X-Ray Diffraction
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D035021:Factor For Inversion Stimulation Protein; D034982:Integration Host Factors; C440502:integration host factor, E coli