Phenotyping of pig alpha 1B-glycoprotein (PO2) and haemopexin by 1D polyacrylamide gel electrophoresis and immunoblotting.
Described is an alternative procedure for the phenotyping of pig alpha 1B-glycoprotein (PO2) and haemopexin. The procedure is based on the separation of serum samples by horizontal polyacrylamide gel electrophoresis, passive blotting onto a nitrocellulose (NC) sheet, and immunochemical detection using a mixture of a primary antibody (rabbit anti-pig alpha 1B or anti-pig haemopexin) and a peroxidase-labelled secondary antibody. Several NC copies can be obtained from a single gel and these can be developed with different monospecific antisera.
Kaláb
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Czechoslovak Academy of Sciences, Department of Genetics, Libĕchov.
Stratil
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D000818:Animals; D001798:Blood Proteins / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D006466:Hemopexin / Q000235:genetics*; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D010641:Phenotype; D013552:Swine
D001798:Blood Proteins; D006466:Hemopexin