Effects of baboon chorionic gonadotrophin antibodies on placental derived baboon trophoblast cells in vitro.
Immunization against chorionic gonadotrophin (CG) can terminate pregnancy in a number of species but the mechanisms responsible for this are poorly understood. Baboon CG has recently been purified and antibodies against this molecule were used in an in vitro system to study their effects on cultured trophoblast cells derived from placentae at 33 days of gestation. Secretion of bCG was measured by radioimmunoassay and the origin of this material was studied using immunohistochemical procedures. Heterologous antibodies against CG were introduced on day 5 of culture. Trophoblast cells appeared to secrete a CG material which was detectable in the daily spent medium and was localized within the trophoblast cells. Addition of antibodies caused cell lysis which was complement dependent. It was concluded that CG was localized on the baboon trophoblast cells and that antibodies against bCG caused complement dependent cell lysis.
Bambra
C S
CS
Institute of Primate Research, National Museums of Kenya, Karen, Nairobi.
Tarara
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R
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000276:immunology*; D003165:Complement System Proteins; D005260:Female; D007124:Immunoenzyme Techniques; D010215:Papio; D011247:Pregnancy; D011863:Radioimmunoassay; D014327:Trophoblasts / Q000166:cytology* / Q000276:immunology
D000906:Antibodies; D006063:Chorionic Gonadotropin; D003165:Complement System Proteins