Prorenin is sorted into the regulated secretory pathway independent of its processing to renin in mouse pituitary AtT-20 cells.
A native human prorenin and a mutant of its processing site (Arg-43 to Gln) were expressed in mouse pituitary AtT-20 cells which process prorenin to renin and have both regulated and constitutive secretory pathways. The native prorenin was processed to renin and secreted in a regulated manner. Although the mutant precursor was not processed, it was also secreted in a regulated manner. These results suggest that prorenin is sorted into the regulated pathway, stored in secretory granules and released by stimulus whether it is processed to renin or not.
Nakayama
K
K
Institute of Biological Sciences, University of Tsukuba, Ibaraki, Japan.
Nagahama
M
M
Kim
W S
WS
Hatsuzawa
K
K
Hashiba
K
K
Murakami
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001120:Arginine; D001483:Base Sequence; D002460:Cell Line; D004792:Enzyme Precursors / Q000235:genetics*; D005973:Glutamine; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D010911:Pituitary Neoplasms; D010957:Plasmids; D011499:Protein Processing, Post-Translational; D012083:Renin / Q000235:genetics*; D015183:Restriction Mapping
D004792:Enzyme Precursors; D005973:Glutamine; D001120:Arginine; D012083:Renin