Protease-catalyzed semisynthesis of human neuropeptide Y.
Human neuropeptide Y was semisynthesized by enzymatic condensation of des-Tyr36-NH2 human neuropeptide Y and H-Tyr-NH2 using Pseudomonas aeruginosa elastase, a metalloenzyme possessing a hydrolytic specificity for the imino side of hydrophobic amino acids. The optimum pH for this enzymatic synthesis was judged to be around 7 in a high concentration of an organic solvent.
Sakina
K
K
Funakoshi
S
S
Yajima
H
H
D000595:Amino Acid Sequence; D006801:Humans; D008969:Molecular Sequence Data; D009478:Neuropeptide Y / Q000096:biosynthesis*; D010447:Peptide Hydrolases / Q000378:metabolism*
D009478:Neuropeptide Y; D010447:Peptide Hydrolases