Transparency of nitrocellulose membranes with Triton X-114.
Transparency of nitrocellulose membranes can be realized by simply impregnating the filters with concentrated Triton X-114. Within a few seconds, protein blots become transparent and can be photographed by transillumination or scanned on a densitometer. Removal of the detergent is possible and the recovered filters can further be used for immunodetection purposes. This procedure is particularly wellsuited where extensive investigation of a single separated protein sample is required.
Vachereau
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Department of Tropical Public Health, Harvard School of Public Health, Boston, MA 02115.
D003101:Collodion; D007158:Immunologic Techniques; D008027:Light; D008567:Membranes, Artificial; D017830:Octoxynol; D011092:Polyethylene Glycols; D011506:Proteins / Q000032:analysis
D008567:Membranes, Artificial; D011506:Proteins; D011092:Polyethylene Glycols; D017830:Octoxynol; D003101:Collodion; C010615:Nonidet P-40