title: Constitutive expression of a native human interferon-alpha 1 gene in Escherichia coli.
abstract: 1. A plasmid for constitutive expression of the human interferon-alpha 1 (hIFN-alpha 1) gene in Escherichia coli is constructed on the basis of the cloning plasmid pBR322 using a strong synthetic promoter, synthetic ribosome binding site and a native hIFN-alpha 1 gene excised from a chromosomal clone. 2. The yield of recombinant hIFN-alpha 1 from E. coli LE392 cells transformed with the expression plasmid pJP1R9-hIFN-alpha 1 is evaluated to be 2-6 x 10(7) U/l bacterial culture for metabolic shaker and 6-8 x 10(7) U/l for fermentor.
authors.affiliation: Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.
authors.forename: I
authors.initials: I
authors.lastname: Ivanov
authors.forename: N
authors.initials: N
authors.lastname: Markova
authors.forename: D
authors.initials: D
authors.lastname: Bachvarov
authors.forename: K
authors.initials: K
authors.lastname: Alexciev
authors.forename: A
authors.initials: A
authors.lastname: Saraffova
authors.forename: V
authors.initials: V
authors.lastname: Maximova
authors.forename: I
authors.initials: I
authors.lastname: Tsaneva
authors.forename: G
authors.initials: G
authors.lastname: Markov
mesh_terms: D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D007370:Interferon Type I / Q000096:biosynthesis* / Q000235:genetics; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins; D015183:Restriction Mapping
chemical_list: D007370:Interferon Type I; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins