N2,N2-dimethylguanosine-specific tRNA methyltransferase contains both nuclear and mitochondrial targeting signals in Saccharomyces cerevisiae.
The TRM1 gene of Saccharomyces cerevisiae encodes a tRNA modification enzyme, N2,N2-dimethylguanosine-specific tRNA methyltransferase, which modifies both mitochondrial and cytoplasmic tRNAs. The enzyme is targeted to mitochondria for the modification of mitochondrial tRNAs. Cellular fractionation and indirect immunofluorescence studies reported here demonstrate that this enzyme is also localized to the nucleus. Further, immunofluorescence experiments using strains that overproduce the enzyme show a staining at the periphery of the nucleus suggesting that the enzyme is found in a subnuclear destination near or at the nuclear membrane. There is no obvious cytoplasmic staining in these overproducing strains. Fusion protein technology was used to begin to localize sequences involved in the nuclear targeting of this enzyme. Indirect immunofluorescence studies indicate that sequences between the first 70 and 213 NH2-terminal amino acids of the methyltransferase are sufficient to target Escherichia coli beta-galactosidase to nuclei.
Li
J M
JM
Department of Biochemistry, School of Medicine, University of Louisville, Kentucky 40292.
Hopper
A K
AK
Martin
N C
NC
D000595:Amino Acid Sequence; D015153:Blotting, Western; D002467:Cell Nucleus / Q000201:enzymology*; D015870:Gene Expression; D005796:Genes; D005800:Genes, Fungal; D008928:Mitochondria / Q000201:enzymology*; D008969:Molecular Sequence Data; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000648:ultrastructure; D012689:Sequence Homology, Nucleic Acid; D012359:tRNA Methyltransferases / Q000032:analysis* / Q000235:genetics
C059863:N(2),N(2)-dimethylguanosine-specific tRNA methyltransferase; D012359:tRNA Methyltransferases