Substrate structural requirements of Schizosaccharomyces pombe RNase P.
RNase P from Schizosaccharomyces pombe has been purified over 2000-fold. The apparent Km for two S. pombe tRNA precursors derived from the supS1 and sup3-e tRNA(Ser) genes is 20 nM; the apparent Vmax is 2.5 nM/min (supS1) and 1.1 nM/min (sup3-e). Processing studies with precursors of other mutants show that the structures of the acceptor stem and anticodon/intron loop of tRNA are crucial for S. pombe RNase P action.
Drainas
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Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511.
Zimmerly
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Willis
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Söll
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D001483:Base Sequence; D004722:Endoribonucleases / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism*; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism*; D012362:RNA, Transfer, Ser / Q000235:genetics / Q000378:metabolism; D043262:Ribonuclease P; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D004718:Saccharomycetales / Q000201:enzymology*; D012568:Schizosaccharomyces / Q000201:enzymology*; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
D012322:RNA Precursors; D012331:RNA, Fungal; D012362:RNA, Transfer, Ser; D004722:Endoribonucleases; D043262:Ribonuclease P