title: Kluyveromyces lactis maintains Saccharomyces cerevisiae intron-encoded splicing signals.
abstract: The actin (ACT) gene from the budding yeast Kluyveromyces lactis was cloned, and the nucleotide sequence was determined. The gene had a single intron 778 nucleotides in length which possessed the highly conserved splicing signals found in Saccharomyces cerevisiae introns. We demonstrated splicing of heterologous ACT transcripts in both K. lactis and S. cerevisiae.
authors.affiliation: Department of Microbiology, University of California, Los Angeles 90024.
authors.forename: J O
authors.initials: JO
authors.lastname: Deshler
authors.forename: G P
authors.initials: GP
authors.lastname: Larson
authors.forename: J J
authors.initials: JJ
authors.lastname: Rossi
mesh_terms: D000199:Actins / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics; D005800:Genes, Fungal; D007438:Introns; D007716:Kluyveromyces / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism; D012326:RNA Splicing; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D004718:Saccharomycetales / Q000235:genetics*
chemical_list: D000199:Actins; D004271:DNA, Fungal; D012322:RNA Precursors