title: ELISA for diagnosis of gnathostomiasis using antigens from Gnathostoma doloresi and G. spinigerum.
abstract: ELISA was developed for the detection of IgG antibody in sera obtained from patients in Japan and in a foreign country. Gnathostoma doloresi adult antigen was less specific than G. spinigerum advanced third-stage larval antigen but their sensitivity were similar. Cross reactivity was observed in Toxocara canis-, Anisakis-, Paragonimus westermani- and Fasciola-infected sera when G. doloresi adult worms but not G. spinigerum advanced third-stage larvae were used as antigens.
authors.affiliation: Department of Parasitology, Faculty of Medicine, Hiroshima University, Japan.
authors.forename: M T
authors.initials: MT
authors.lastname: Anantaphruti
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006039:Gnathostoma / Q000276:immunology*; D006801:Humans; D009349:Nematode Infections / Q000175:diagnosis / Q000276:immunology*; D012680:Sensitivity and Specificity; D013802:Thelazioidea / Q000276:immunology*
chemical_list: D000947:Antigens, Helminth