title: Characterization of botulinum type A neurotoxin gene: delineation of the N-terminal encoding region.
abstract: A 456 basepair HindIII fragment that encoded a portion of the type A botulinum neurotoxin gene was cloned into Escherichia coli using a plasmid vector. DNA sequence analysis revealed that this botulinum DNA insert encoded an open reading frame of 35 amino acid residues of which 34 corresponded to the N-terminal residues of botulinum neurotoxin type A.
authors.affiliation: Department of Bacteriology, University of Wisconsin, Madison 53706.
authors.forename: M J
authors.initials: MJ
authors.lastname: Betley
authors.forename: E
authors.initials: E
authors.lastname: Somers
authors.forename: B R
authors.initials: BR
authors.lastname: DasGupta
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001905:Botulinum Toxins / Q000235:genetics*; D003001:Cloning, Molecular; D003014:Clostridium botulinum / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000235:genetics*; D015345:Oligonucleotide Probes
chemical_list: D009498:Neurotoxins; D015345:Oligonucleotide Probes; D001905:Botulinum Toxins