Comparison of properties of collected cells and cells from the culture vessel during continuous culture of Streptococcus mutans Ingbritt.
In continuous-culture studies chemostat effluents are usually collected into a receiving flask in an ice bath to obtain enough cells for an experiment. It is assumed that the properties of these are not significantly different from those of the culture in the chemostat vessel. This assumption has been tested for the dental pathogen Streptococcus mutans Ingbritt. Collected supernatant fluid and cells were compared with supernatant fluid and cells taken directly from the culture vessel, for four major groups of culture properties: viability and biomass, concentrations of metabolites and nutrients, activities of selected enzymes, and glycolytic rates. The assumption held true except for glycolytic rate during endogenous metabolism. It is suggested that comparison of collected and culture vessel cells is an important control which should be done in all continuous culture studies of microbial physiology and biochemistry, but that the properties of Strep. mutans cells collected on ice up to 16 h do reflect those of cells actively growing in the chemostat.
Mayo
J A
JA
Pathogenic Microbes Research Laboratory, PHLS Centre for Applied Microbiology and Research, Porton Down, Salisbury, UK.
McDermid
A S
AS
Marsh
P D
PD
Ellwood
D C
DC
D001431:Bacteriological Techniques; D005941:Glucokinase / Q000378:metabolism; D006019:Glycolysis; D006863:Hydrogen-Ion Concentration; D013295:Streptococcus mutans / Q000201:enzymology / Q000378:metabolism* / Q000502:physiology
D005941:Glucokinase