Presence of endothelin-1 in porcine spinal cord: isolation and sequence determination.
We investigated the molecular forms of endothelin (ET) related peptides in porcine spinal cord by high performance liquid chromatography coupled with radioimmunoassays using three antisera raised against ET-1 and C-terminal fragments of ET-1 and big ET-1. ET-1 and its oxidized form were isolated as major immunoreactive peptides and sequenced. Furthermore, immunoreactivities like ET-3 and big ET-1(22-39) (contents: less than 8% and less than 1% of ET-1, respectively) were detected based on their chromatographic retention times and characteristics of immunoreactivity to the antisera. Big ET-1 was only scarcely detected. Immunohistochemical study showed the presence of ET-1-like immunoreactivity in motoneurons, dorsal horn neurons and dot- and fiber-like structures in the dorsal horn of lumbar spinal cord. These results indicate that ET-1 is present not only in endothelial cells but also in spinal cord, and that big ET-1 is converted into ET-1 in spinal cord by specific processing between Trp21-Val22. The data also indicate that ET-1 may act as a neuropeptide in the central nervous system.
Shinmi
O
O
Department of Biochemistry, University of Tsukuba, Ibaraki, Japan.
Kimura
S
S
Yoshizawa
T
T
Sawamura
T
T
Uchiyama
Y
Y
Sugita
Y
Y
Kanazawa
I
I
Yanagisawa
M
M
Goto
K
K
Masaki
T
T
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D016232:Endothelins; D004730:Endothelium, Vascular / Q000032:analysis*; D008297:Male; D008969:Molecular Sequence Data; D009046:Motor Neurons / Q000032:analysis; D010446:Peptide Fragments / Q000302:isolation & purification; D010455:Peptides / Q000302:isolation & purification*; D011863:Radioimmunoassay; D013116:Spinal Cord / Q000032:analysis*; D013552:Swine; D013556:Swine, Miniature; D014662:Vasoconstrictor Agents / Q000302:isolation & purification*
D016232:Endothelins; D010446:Peptide Fragments; D010455:Peptides; D014662:Vasoconstrictor Agents