Enhanced monocyte progenitor cell proliferation in bone marrow of hyperlipemic swine.
This study demonstrated for the first time that bone marrow is a target of enhanced in vivo monocytopoiesis in hyperlipemia. A significantly greater number of bone marrow cells (BMC) were recovered per femur in swine fed a hyperlipemic (HL) diet compared with swine fed a normal (N) diet. In addition, a significantly elevated number of monocytic precursors proliferated in HL-swine compared with N-swine BMC cultures grown in standardized media in the absence of an exogenous source of colony stimulating factor (CSF). HL-swine sera stimulated a significant enhancement in the number of proliferating monocytic precursor cells, regardless of whether or not the BMC were from HL- or N-swine. In addition, HL-swine compared with N-swine BMC demonstrated an enhanced intrinsic capacity to form monocytic colonies in culture, irrespective of the source of swine sera used to stimulate growth.
Averill
L E
LE
Cardiovascular Research Center, Cleveland Research Institute, Ohio 44115.
Meagher
R C
RC
Gerrity
R G
RG
D000818:Animals; D001853:Bone Marrow / Q000201:enzymology / Q000473:pathology*; D002455:Cell Division; D003115:Colony-Stimulating Factors / Q000378:metabolism; D004950:Esterases / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000201:enzymology / Q000473:pathology*; D006651:Histocytochemistry; D006949:Hyperlipidemias / Q000201:enzymology / Q000473:pathology*; D008214:Lymphocytes / Q000201:enzymology / Q000473:pathology; D008297:Male; D009000:Monocytes / Q000201:enzymology / Q000473:pathology*; D009504:Neutrophils / Q000201:enzymology / Q000473:pathology; D013552:Swine
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D004950:Esterases