title: The effect of L-tryptophan on daytime sleep latency in normals: correlation with blood levels.
abstract: L-Tryptophan, an essential amino acid, is readily converted to serotonin, which is thought to be important for expression of slow wave sleep and possibly rapid eye movement (REM) sleep. A vast but often confusing literature exists on L-tryptophan effects on inducing, maintaining, or altering sleep. In this study we measured the effects of L-tryptophan on objective (multiple sleep latency) and subjective [Stanford Sleepiness Scale (SSS)] measures of sleepiness and examined their relationship to blood L-tryptophan levels. Ten healthy volunteers (eight men and two women; mean +/- SD age 34 +/- 10 years) received placebo or 1.2 or 2.4 g or L-tryptophan on separate days in random double-blind fashion. Sleep latency and SSS were measured initially and at 60 and 120 min after ingestion. Blood and urine were collected at regular intervals. Compared with placebo both L-tryptophan doses reduced sleep latency at 1 h, with the reduction persisting at 2 h for the 2.4-g dose only (p less than 0.05). There was a positive correlation between subjective and objective sleepiness measures but only with the 2.4-g dose (rs = 0.76, p less than 0.01). There was a highly significant correlation between blood L-tryptophan and sleep latency at 0, 60, and 120 min in all subjects for all drug conditions (r = 0.276, df = 79, p = 0.013). Very small amounts of free L-tryptophan or its metabolites were found in the urine, with the exception of kynurenic acid. We conclude that L-tryptophan consistently reduced sleep latency in normals and that this correlates with blood levels.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Section of Respiratory Medicine, University of Manitoba, Winnipeg, Canada.
authors.forename: C F
authors.initials: CF
authors.lastname: George
authors.forename: T W
authors.initials: TW
authors.lastname: Millar
authors.forename: P J
authors.initials: PJ
authors.lastname: Hanly
authors.forename: M H
authors.initials: MH
authors.lastname: Kryger
mesh_terms: D000328:Adult; D002940:Circadian Rhythm / Q000187:drug effects*; D002986:Clinical Trials as Topic; D004305:Dose-Response Relationship, Drug; D004311:Double-Blind Method; D004569:Electroencephalography; D005071:Evoked Potentials / Q000187:drug effects; D005260:Female; D006801:Humans; D008297:Male; D008657:Metabolic Clearance Rate; D011897:Random Allocation; D011930:Reaction Time / Q000187:drug effects*; D012894:Sleep Stages / Q000187:drug effects*; D014364:Tryptophan / Q000493:pharmacokinetics / Q000494:pharmacology*
chemical_list: D014364:Tryptophan