The primary structure of porcine liver acylamino acid-releasing enzyme deduced from cDNA sequences.
Two overlapping cloned cDNAs encoding the entire amino acid sequences of the subunits of acylamino acid-releasing enzyme (AARE) [EC 3.4.19.1] have been isolated from porcine liver cDNA lambda gt10 cDNA libraries and sequenced. Sequence analyses of the cDNA and several Achromobacter protease I-digested peptides of the purified protein revealed that porcine liver AARE consists of four identical subunits, and each comprising a single chain of 732 amino acids with acetylmethionine at the N-terminus.
Mitta
M
M
Biotechnology Research Laboratories, Takara Shuzo Co., Ltd., Shiga.
Asada
K
K
Uchimura
Y
Y
Kimizuka
F
F
Kato
I
I
Sakiyama
F
F
Tsunasawa
S
S
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases / Q000235:genetics*; D015183:Restriction Mapping; D013552:Swine
D004247:DNA; D010447:Peptide Hydrolases; C038220:acylaminoacyl-peptidase