title: Isolation of cardiac ventricular myocytes from newborn rats by use of fractional elutriation centrifugation.
abstract: Isolation of cardiac ventricular myocytes from newborn rats (0-4 days old) by use of elutriation centrifugation is described. By the use of fractional centrifugation, a homogeneous myocyte population with high purity was obtained without any further procedures, yielding about 10(6) myocytes/rat. When 48-hr monolayer cultures were established, the cells showed normal pulsatory contractions. A morphological evaluation of such cultures is given.
authors.affiliation: Department of Pharmacology, University of Copenhagen, Denmark.
authors.forename: B
authors.initials: B
authors.lastname: Diamant
authors.forename: E
authors.initials: E
authors.lastname: Steiness
authors.forename: B F
authors.initials: BF
authors.lastname: Hansen
authors.forename: M B
authors.initials: MB
authors.lastname: Jensen
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D002478:Cells, Cultured; D002498:Centrifugation; D003584:Cytological Techniques; D009124:Muscle Proteins / Q000378:metabolism; D009206:Myocardium / Q000166:cytology*; D010316:Particle Size; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D009124:Muscle Proteins