title: Genome organization in hybrids between prophage phi 80 and Escherichia coli virus phi gamma.
abstract: Prophage phi 80 is used for the detection of "discrete" viruses such as phage phi gamma by genetic recombination. Several genetic events have produced a series of hybrids which are currently being characterized. Characteristic genomic properties of the parent phages are recognized in these hybrids. "lambda"-type phages contain a cos-ended DNA of a single sequence, the size of which may be altered by hybridization. "gamma"-type phages contain DNA molecules of uniform size (about 52 kb) and of variable ends; their genome is able to promote highly efficient transduction (pug type) regardless of the origin of the right arm. In both hybrids, the att site is most often a junction point between the parental genomes due to common int-promoted recombination. New evidence is provided for the formation of viable heterozygous "lambda/gamma"-type bacteriophages.
authors.affiliation: Laboratoire de Microbiologie, Faculté de Médecine ULB, Bruxelles.
authors.forename: J P
authors.initials: JP
authors.lastname: Gratia
mesh_terms: D001435:Bacteriophages / Q000235:genetics*; D004279:DNA, Viral / Q000032:analysis; D004926:Escherichia coli; D005814:Genes, Viral; D006824:Hybridization, Genetic; D008242:Lysogeny; D011533:Proviruses / Q000235:genetics*; D014161:Transduction, Genetic; D014771:Virion / Q000235:genetics
chemical_list: D004279:DNA, Viral