title: Distribution of adenosine deaminase-complexing protein in murine tissues.
abstract: It has been suggested that mouse and rat lack adenosine deaminase-complexing protein because in these species exclusively the small molecular weight form of adenosine deaminase (ADA-S) is found. This suggestion is based on the assumption that the adenosine deaminase binding capacity is an inherent functional characteristic of adenosine deaminase-complexing protein. We report on the presence of adenosine deaminase-complexing protein immunoreactivity in mouse and rat determined with a species cross-reactive polyclonal anti-adenosine deaminase-complexing protein serum. In the mouse the tissue and subcellular distribution and the electrophoretic mobility in starch and polyacrylamide gels of the protein correspond with those of adenosine deaminase-complexing protein, but it does not bind the small molecular weight form of adenosine deaminase. Furthermore, in human, mouse, and rat kidney cortex adenosine deaminase and adenosine deaminase-complexing protein did not colocalize by immunohistochemistry. It is suggested that the function of adenosine deaminase-complexing protein is not adenosine deaminase-related.
authors.affiliation: Department of Pathology, University of Limburg, Maastricht, The Netherlands.
authors.forename: W N
authors.initials: WN
authors.lastname: Dinjens
authors.forename: J
authors.initials: J
authors.lastname: ten Kate
authors.forename: J T
authors.initials: JT
authors.lastname: Wijnen
authors.forename: E P
authors.initials: EP
authors.lastname: van der Linden
authors.forename: C J
authors.initials: CJ
authors.lastname: Beek
authors.forename: M H
authors.initials: MH
authors.lastname: Lenders
authors.forename: P M
authors.initials: PM
authors.lastname: Khan
authors.forename: F T
authors.initials: FT
authors.lastname: Bosman
mesh_terms: D000243:Adenosine Deaminase; D000818:Animals; D018819:Dipeptidyl Peptidase 4; D004592:Electrophoresis, Starch Gel; D004848:Epithelium / Q000201:enzymology; D006023:Glycoproteins; D006801:Humans; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D007421:Intestine, Small / Q000201:enzymology*; D007527:Isoenzymes / Q000032:analysis* / Q000302:isolation & purification; D007672:Kidney Cortex / Q000201:enzymology*; D007687:Kidney Tubules, Proximal / Q000201:enzymology; D008099:Liver / Q000201:enzymology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D006023:Glycoproteins; D007106:Immune Sera; D007527:Isoenzymes; C042807:DPP4 protein, human; D018819:Dipeptidyl Peptidase 4; D000243:Adenosine Deaminase