Hormonal regulation of malic enzyme expression in primary cultures of foetal brown adipocytes.
Insulin increased malic enzyme activity and protein content in foetal brown adipocyte primary cultures through a two fold increase in the rates of enzyme synthesis. Conversely, noradrenaline prevented the induction caused by insulin on enzyme expression through an accelerate rate of malic enzyme degradation. Tri-iodothyronine had no effect on the expression of malic enzyme in cultured foetal brown adipocytes.
Lorenzo
M
M
Departamento e Bioquímica y Biología Molecular, Facultad de Farmacia, Ciudad Universitaria, Madrid, Spain.
Fabregat
I
I
Benito
M
M
D002001:Adipose Tissue, Brown / Q000196:embryology / Q000201:enzymology*; D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D007328:Insulin / Q000494:pharmacology; D008291:Malate Dehydrogenase / Q000276:immunology / Q000502:physiology*; D009638:Norepinephrine / Q000494:pharmacology; D014176:Protein Biosynthesis; D051381:Rats
D007328:Insulin; D008291:Malate Dehydrogenase; D009638:Norepinephrine