Rapid decay of serum IgG recognizing gram-negative cell wall core antigens in neonatal calves.
Serum immunoglobulins of the IgG isotype recognizing common gram-negative cell core epitopes were serially measured, using a direct ELISA, on samples obtained from 20 neonatal Holstein calves. An R-mutant Escherichia coli (strain J5) was used as a plate antigen in this assay. Total serum IgG concentration was measured using radial immunodiffusion. Half-lives of core antigen-specific IgG (7.56 days) and total serum IgG (22.66 days) were dramatically different (P less than 0.0005). This may be an indication of cross-reactive consumption of core antigen-specific immunoglobulins.
Douglas
V L
VL
Department of Clinical Pathology, School of Veterinary Medicine, University of California, Davis 95616.
Cullor
J S
JS
Tyler
J W
JW
Thurmond
M C
MC
Bushnell
R B
RB
D000818:Animals; D000831:Animals, Newborn / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology*; D002417:Cattle / Q000276:immunology*; D002473:Cell Wall / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D004926:Escherichia coli / Q000276:immunology*; D006207:Half-Life; D007074:Immunoglobulin G / Q000032:analysis* / Q000276:immunology; D013997:Time Factors
D000942:Antigens, Bacterial; D007074:Immunoglobulin G