Control of gene expression by oligonucleotides covalently linked to intercalating agents.
Oligodeoxynucleotides covalently linked to intercalating agents selectively recognize the complementary sequence of the oligonucleotide. The intercalating agent provides an additional binding energy which stabilizes the complex. These substances can be used in vitro to block mRNA translation. In cell cultures they are able to inhibit the cytopathic effect of viruses, such as influenza virus and the oncogenic virus SV40. They kill trypanosomes in culture as a result of protein synthesis inhibition. A reactive group can be attached to an oligodeoxynucleotide in order to achieve site-directed modifications of the target sequence. Metal complexes of EDTA, phenanthroline or porphyrins induce cleavage reactions of the phosphodiester backbone in both DNA and RNA. Photoactive groups can be used to modify bases in the complementary sequence. The double helix can be recognized and modified by oligonucleotides that bind to the major groove, forming a local triple helix. These site-directed modifications may inhibit biological processes. The oligonucleotide can be made resistant to nuclease digestion by substituting the synthetic alpha-anomers of nucleosides to the natural beta-nucleosides. These results provide the basis for the design of gene-specific inhibitors that can be used as tools in molecular and cellular biology. They also suggest new approaches for the rational development of selective anti-viral, anti-parasitic, and anti-tumoral agents.
Hélène
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C
Institut national de la santé et de la recherche médicale, Muséum national d'histoire naturelle, Paris, France.
Thuong
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NT
D000970:Antineoplastic Agents; D000981:Antiprotozoal Agents; D000998:Antiviral Agents; D004249:DNA Damage; D003851:Deoxyribonucleases; D005786:Gene Expression Regulation / Q000187:drug effects*; D007364:Intercalating Agents; D009841:Oligonucleotides / Q000494:pharmacology*; D016376:Oligonucleotides, Antisense; D014176:Protein Biosynthesis / Q000187:drug effects; D012260:Ribonucleases
D000970:Antineoplastic Agents; D000981:Antiprotozoal Agents; D000998:Antiviral Agents; D007364:Intercalating Agents; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; D003851:Deoxyribonucleases; D012260:Ribonucleases