Biosynthesis of interleukin-I beta at inflammatory site in rabbits: kinetics and producing cells.
Biosynthesis of interleukin-I beta (IL-I beta) by inflammatory peritoneal exudate cells (PEC) was studied in terms of biological activity (thymocyte co-mitogenic assay), IL-I beta mRNA expression (cytoplasmic slot blot analysis) and detection of immunoreactive IL-I beta molecule (immunocytochemistry). Our findings were taken to conclude that IL-I beta expression was observed only in PEC harvested from early inflammatory site and the kinetic profile of the IL-I activity during the course of the inflammation was explained by IL-I beta mRNA expression, namely de-novo synthesis by inflammatory exudate cells. With respect to a single cell level, it was definitely proved that polymorphonuclear leucocytes were the major producer of IL-I beta during the casein-induced acute inflammation in rabbits.
Goto
F
F
Department of Pathology, Kumamoto University Medical School, Japan.
Goto
K
K
Mori
S
S
Ohkawara
S
S
Yoshinaga
M
M
D000818:Animals; D001202:Ascitic Fluid / Q000276:immunology*; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000235:genetics; D007700:Kinetics; D007962:Leukocytes / Q000276:immunology; D008297:Male; D009504:Neutrophils / Q000276:immunology*; D010538:Peritonitis / Q000276:immunology*; D012333:RNA, Messenger / Q000032:analysis; D011817:Rabbits
D007375:Interleukin-1; D012333:RNA, Messenger