title: Structural changes in the tracheae of preterm lambs induced by ventilation.
abstract: Compliant immature airways sustain significant deformation following positive pressure ventilation. To evaluate the structural changes induced by in vivo positive pressure ventilation, tracheae of preterm lambs (107-116 d gestational age) were studied histologically. Nonventilated (group I: n = 7) and ventilated (group II: n = 7) tracheal segments were excised and studied by histologic and morphometric techniques. Computerized image analysis was used to measure dimensions of tracheal wall components and of the tracheal section. The circumference, diameter, and cross-sectional area of the section as well as the length of the trachealis muscle were significantly greater; although the thickness of the muscle and cartilage were seen to be significantly lower in group II sections compared to group I sections. Also, in comparison to group I, in group II sections there was lesser overlap of the posterior free ends of tracheal cartilage and the epithelial layer was flattened and focally abraded. Our findings demonstrate structural changes in the airway of preterm animals and characterize alterations in the geometric arrangement of muscle and cartilage after PPV. These results suggest possible structural mechanisms for the functional changes seen during and subsequent to mechanical ventilation.
authors.affiliation: Department of Physiology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.
authors.forename: K S
authors.initials: KS
authors.lastname: Deoras
authors.forename: M R
authors.initials: MR
authors.lastname: Wolfson
authors.forename: V K
authors.initials: VK
authors.lastname: Bhutani
authors.forename: T H
authors.initials: TH
authors.lastname: Shaffer
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D004848:Epithelium / Q000293:injuries / Q000473:pathology; D011175:Positive-Pressure Respiration / Q000009:adverse effects*; D012756:Sheep; D014132:Trachea / Q000293:injuries* / Q000473:pathology