Prediction of early kidney transplant rejection by a crossmatch with donor skin.
Immunofluorescence staining of donor skin with recipient serum was performed in 25 patients undergoing kidney transplantation. Transplants were performed when cytotoxicity T cell crossmatches with long incubation and with antiglobulin enhancement were negative. In 20 patients the skin crossmatch was negative and all had an uneventful course. In 5 other recipients, the crossmatch with skin was positive and all developed severe rejection 1-8 days after transplantation. The correlation between skin crossmatch and early rejection was statistically highly significant (P less than 0.00001). Rejection in the skin-crossmatch-positive group led to graft loss in 3, and chronic rejection with poor residual function in the other 2 patients. Serum creatinine at one month was in the 2.6-5.0 mg/dl range in these two patients, while in the group with negative skin crossmatches 15 had serum creatinine levels of less than 1.6, and 5 were in the 1.6-2.5 mg/dl range. Although the nature of the skin antigen is unknown, it appears that this crossmatch procedure was highly accurate in predicting early kidney transplant rejection.
Moraes
J R
JR
Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas 75235.
Pettaway
C
C
Stastny
P
P
D005455:Fluorescent Antibody Technique; D006084:Graft Rejection; D006650:Histocompatibility Testing; D006801:Humans; D016030:Kidney Transplantation; D012867:Skin / Q000276:immunology*