Salmonella typhimurium virulence in a burned-mouse model.
Various features of salmonellosis were examined in a burned-mouse model. In this model, which uses an outbred mouse strain, a challenge dose of ca. 100 CFU with any of several strains of Salmonella typhimurium caused a fatal infection. A variety of mutated strains attenuated for virulence in Salmonella-susceptible parenterally infected mice were also attenuated in the burned-mouse model. When administered as live vaccines injected intraperitoneally the same attenuated strains provided between slight and complete protection against subsequent lethal challenge subcutaneously at the site of a burn. The correspondence of results obtained in the burned-mouse model with those seen in other mouse models coupled with the unique advantages of the burned-mouse model argue for the usefulness of the model in studies of salmonellosis and in testing of strains constructed for use as live vaccines.
Carsiotis
M
M
Department of Molecular Genetics, University of Cincinnati College of Medicine, Ohio 45267-0524.
Stocker
B A
BA
Holder
I A
IA
D000818:Animals; D001428:Bacterial Vaccines / Q000008:administration & dosage; D002056:Burns / Q000276:immunology / Q000382:microbiology*; D004195:Disease Models, Animal; D005260:Female; D007113:Immunity, Innate; D051379:Mice; D009154:Mutation; D012481:Salmonella Infections, Animal / Q000276:immunology / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000235:genetics / Q000472:pathogenicity*; D014774:Virulence
D001428:Bacterial Vaccines