title: Fluoroimmunoassay studies with solubilized antigens from Borrelia burgdorferi.
abstract: Sodium deoxycholate-solubilized Borrelia burgdorferi antigen was prepared for use in a solid-phase fluoroimmunoassay (FIA-L) to detect antibodies in Lyme disease. Serum specimens were tested by FIA-L and by a microimmunofluorescence test. The FIA-L results are comparable to those of the standard microimmunofluorescence test. The overall agreement was 0.98. Moreover, the FIA-L procedure is simple and rapid; fluorescence is objectively determined and is proportional to antibody titer.
authors.affiliation: Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509.
authors.forename: K E
authors.initials: KE
authors.lastname: Hechemy
authors.forename: H L
authors.initials: HL
authors.lastname: Harris
authors.forename: J A
authors.initials: JA
authors.lastname: Wethers
authors.forename: R W
authors.initials: RW
authors.lastname: Stevens
authors.forename: B R
authors.initials: BR
authors.lastname: Stock
authors.forename: A A
authors.initials: AA
authors.lastname: Reilly
authors.forename: J L
authors.initials: JL
authors.lastname: Benach
mesh_terms: D000907:Antibodies, Bacterial / Q000032:analysis*; D000942:Antigens, Bacterial / Q000276:immunology*; D001898:Borrelia / Q000276:immunology*; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D006801:Humans; D015151:Immunoblotting; D008193:Lyme Disease / Q000175:diagnosis*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial