Endogenous DNA damage as related to cancer and aging.
The endogenous background level of oxidant-induced DNA damage in vivo has been assayed by measuring 8-hydroxydeoxyguanosine (oh8dG), thymine glycol and thymidine glycol in urine and oh8dG in DNA. The level of oxidative DNA damage as measured by oh8dG in normal rat liver is shown to be extensive (1/130,000 bases in nuclear DNA and 1/8000 bases in mitochondrial DNA), especially in mtDNA. The methylation adduct 7-methylguanine (m7G) has also been found. m7G is one of about 5 adducts found on methylating DNA, and oh8dG is one of about 20 adducts found on oxidizing DNA, e.g., by radiation. We also discuss 3 hitherto unrecognized antioxidants in man.
Ames
B N
BN
Department of Biochemistry, University of California, Berkeley 94720.
D000375:Aging / Q000235:genetics*; D000818:Animals; D000975:Antioxidants; D004249:DNA Damage; D004260:DNA Repair; D006801:Humans; D008760:Methylguanidine / Q000378:metabolism; D009369:Neoplasms / Q000235:genetics*; D010084:Oxidation-Reduction; D051381:Rats
D000975:Antioxidants; D008760:Methylguanidine