title: A dual microtiter plate (192 sample) luminometer employing computer-aided single-photon imaging applicable to cellular luminescence and luminescence immunoassay.
abstract: In spite of the increasing applications of luminescence measurements, no instrumentation is yet commercially available that permits sensitive luminescence measurements to be performed simultaneously on a large number of samples. Based on the principle of single-photon imaging, we describe here the performance of a computer-aided image luminometer with a capacity for two microtiter plates. Applications to the study of chemiluminescence by a B lymphocyte cell line, and to an IgE luminescence immunoassay are used to exemplify the capabilities of the system, which we have termed 'chemiluminescence multiwell analyser'.
authors.affiliation: Institute of Clinical Immunology, Inselspital Bern, Switzerland.
authors.forename: F E
authors.initials: FE
authors.lastname: Maly
authors.forename: M
authors.initials: M
authors.lastname: Vittoz
authors.forename: A
authors.initials: A
authors.lastname: Urwyler
authors.forename: K
authors.initials: K
authors.lastname: Koshikawa
authors.forename: L
authors.initials: L
authors.lastname: Schleinkofer
authors.forename: A L
authors.initials: AL
authors.lastname: De Weck
mesh_terms: D001402:B-Lymphocytes / Q000032:analysis; D002460:Cell Line; D003201:Computers; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D007124:Immunoenzyme Techniques; D007073:Immunoglobulin E / Q000032:analysis; D008027:Light; D008163:Luminescent Measurements
chemical_list: D007073:Immunoglobulin E