Yeast 2 micron vectors replicate and undergo recombination in Torulaspora delbrueckii.
In order to develop a procedure for transformation of the industrial yeast Torulaspora delbrueckii, we have constructed a set of recombinant plasmids carrying Saccharomyces cerevisiae ARS and 2 microns origin of replication and kanamycin-G418 resistance gene of Tn903(601) as a selective marker. In this paper we show that S. cerevisiae ARS vectors can replicate autonomously and that vectors bearing the whole S. cerevisiae 2 microns sequence yield stable transformants. We also present evidence to show that 2 microns vectors undergo an FLP-mediated inter- and intramolecular recombination, which suggests that T. delbrueckii can support the amplification and partition mechanisms of these plasmids.
Compagno
C
C
Dipartimento di Fisiologia e Biochimica Generali, Universita' di Milano, Italy.
Ranzi
B M
BM
Martegani
E
E
D015139:Blotting, Southern; D015870:Gene Expression; D005822:Genetic Vectors; D007763:Lac Operon; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D015183:Restriction Mapping; D012440:Saccharomyces / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014170:Transformation, Genetic