Feline hybridoma growth factor/interleukin-6 activity.
An assay system was developed to measure feline hybridoma growth factor (HGF)/interleukin-6 (IL-6) activity in biological samples containing many kinds of cytokines by using the proliferation of the newly established mouse-rat hybridoma clone, B3B1. The proliferative response of this B3B1 clone was IL-6-specific, and could not be promoted by other cytokines including IL-1, IL-2, IL-3, and granulocyte-colony-stimulating factor (G-CSF). The anti-human B-cell stimulatory factor 2 (BSF-2)/IL-6 antiserum did not neutralize feline HGF/IL-6 activity in conditioned media prepared from feline con A-stimulated splenocytes and unstimulated alveolar macrophages, indicating antigenic differences between species. Feline HGF/IL-6 was eluted into the fractions corresponding to a molecular weight of 30,000-40,000 in gel filtration, and into the fractions at a salt concentration of 0.2-0.3 M NaCl in anion exchange chromatography. The physicochemical properties of feline HGF/IL-6 were slightly different from those of murine and human IL-6.
Ohashi
T
T
Department of Veterinary Internal Medicine, Faculty of Agriculture, University of Tokyo, Japan.
Goitsuka
R
R
Ono
K
K
Hasegawa
A
A
D000818:Animals; D001685:Biological Factors / Q000494:pharmacology; D002415:Cats / Q000502:physiology*; D002455:Cell Division / Q000187:drug effects; D002852:Chromatography, Ion Exchange; D003470:Culture Media; D016207:Cytokines; D006133:Growth Substances / Q000502:physiology*; D006825:Hybridomas / Q000166:cytology; D007158:Immunologic Techniques; D015850:Interleukin-6 / Q000502:physiology*; D051379:Mice; D008970:Molecular Weight; D011994:Recombinant Proteins / Q000494:pharmacology
D001685:Biological Factors; D003470:Culture Media; D016207:Cytokines; D006133:Growth Substances; D015850:Interleukin-6; D011994:Recombinant Proteins