title: Estimating the degree of infection of Plasmodium berghei infected red blood cells by evaluation of pyruvate kinase activity.
abstract: A procedure is proposed to estimate the parasitemia of red blood cells infected with P. berghei by determination of pyruvate kinase activity. The activity of this enzyme shows a linear dependence not on the relative number of infected cells but on the average number of parasites per red blood cell. To provide a transformation between these two parameters a theoretical model of multiple infections of blood cells is derived, the accuracy of which was proved experimentally.
authors.affiliation: Institute of Biochemistry, School of Medicine (Charité), Humboldt-University, Berlin, G.D.R.
authors.forename: D
authors.initials: D
authors.lastname: Buckwitz
authors.forename: G
authors.initials: G
authors.lastname: Jacobasch
authors.forename: M
authors.initials: M
authors.lastname: Break
mesh_terms: D000818:Animals; D004912:Erythrocytes / Q000201:enzymology* / Q000469:parasitology; D008288:Malaria / Q000201:enzymology* / Q000469:parasitology; D051379:Mice; D010962:Plasmodium berghei / Q000302:isolation & purification; D011336:Probability; D011770:Pyruvate Kinase / Q000378:metabolism*; D051381:Rats; D012044:Regression Analysis
chemical_list: D011770:Pyruvate Kinase