Klebsiella pneumoniae haemolysin adsorption to red blood cells.
It was necessary to incubate the Klebsiella pneumoniae haemolysin with erythrocytes at 37 degrees C to produce the whole lytic action. The amount of attached klebolysin at 4 degrees C increased as its concentration in the medium increased, until the erythrocyte surface was saturated. Treatment with 2-mercaptoethanol was necessary to permit the adsorption; it was inhibited by low concentrations of cholesterol. Klebsolysin was immunogenic and its antiserum neutralized its own haemolytic effect and streptolysin O. Anti-streptolysin serum also neutralized klebsolysin. Streptolysin O attachment to erythrocytes impeded the posterior klebolysin adsorption in the same way that klebsolysin adsorption interfered with streptolysin O attachment.
Albesa
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Departamento de Microbiologíae Inmunología, Facultad de Ciencias Exactas, Físico, Químicas y Naturales, Universidad Nacional de Río Cuarto, Argentina.
D000327:Adsorption; D000818:Animals; D004912:Erythrocytes / Q000378:metabolism*; D006460:Hemolysin Proteins / Q000378:metabolism*; D006461:Hemolysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007106:Immune Sera / Q000276:immunology; D007711:Klebsiella pneumoniae / Q000378:metabolism*; D011817:Rabbits; D013696:Temperature
D006460:Hemolysin Proteins; D007106:Immune Sera