title: Establishment and characterization of human glioblastoma cell lines in vitro and their xenografts in nude mice.
abstract: Human gliomas were grown as permanent tissue cultures and xenografts in nude mice. Growth kinetics, immunohistology and karyotypes were established. One tumor showed a distinct change in its karyotype after only two passages in nude mice; by contrast, no change of immunophenotype occurred during and after in vivo passaging. Treatment of this glioma as xenograft in nude mice revealed a high sensitivity towards single agent treatment with BCNU. In view of the cytologic aberrations, however, this result should be interpreted cautiously with regard to the clinical situation.
authors.affiliation: Institute of Virus Research, German Cancer Research Center.
authors.forename: H
authors.initials: H
authors.lastname: Fischer
authors.forename: W J
authors.initials: WJ
authors.lastname: Zeller
authors.forename: K
authors.initials: K
authors.lastname: Schwechheimer
authors.forename: K J
authors.initials: KJ
authors.lastname: Hutter
authors.forename: B
authors.initials: B
authors.lastname: Wowra
authors.forename: S
authors.initials: S
authors.lastname: Kunze
authors.forename: V
authors.initials: V
authors.lastname: Sturm
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000188:drug therapy / Q000473:pathology* / Q000503:physiopathology*; D002330:Carmustine / Q000627:therapeutic use; D004273:DNA, Neoplasm / Q000032:analysis; D005434:Flow Cytometry; D005910:Glioma / Q000188:drug therapy / Q000473:pathology / Q000503:physiopathology*; D006801:Humans; D007621:Karyotyping; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014407:Tumor Cells, Cultured
chemical_list: D004273:DNA, Neoplasm; D002330:Carmustine