title: Cloning and expression of a functional fucose-specific lectin from an orange peel mushroom, Aleuria aurantia.
abstract: Aleuria aurantia lectin (AAL) shows sugar-binding specificity for L-fucose. A lambda gt11 expression library was constructed from A. aurantia poly(A) RNA and screened with a polyclonal antiserum directed against AAL. An immunopositive clone carrying 1.3-kb EcoRI fragment was obtained. The fragment encoded AAL, but lacked a nucleotide sequence corresponding to the two amino-terminal amino acids. The 5'-terminal part of the fragment was replaced with a chemically synthesized DNA fragment and inserted into an expression vector to yield a plasmid pKA-1. Escherichia coli carrying pKA-1 expressed functional AAL and the recombinant AAL showed the same immunological properties as those of natural AAL.
authors.affiliation: Research Institute, Nichirei Co., Tokyo, Japan.
authors.forename: F
authors.initials: F
authors.lastname: Fukumori
authors.forename: N
authors.initials: N
authors.lastname: Takeuchi
authors.forename: T
authors.initials: T
authors.lastname: Hagiwara
authors.forename: K
authors.initials: K
authors.lastname: Ito
authors.forename: N
authors.initials: N
authors.lastname: Kochibe
authors.forename: A
authors.initials: A
authors.lastname: Kobata
authors.forename: Y
authors.initials: Y
authors.lastname: Nagata
mesh_terms: D000595:Amino Acid Sequence; D001203:Ascomycota / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D037102:Lectins / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D015183:Restriction Mapping
chemical_list: D037102:Lectins; C027112:fucose-binding lectin