A simple method to determine released acetylcholine in the presence of choline.
In experiments where the release of labeled acetylcholine is to be determined, the addition of choline oxidase to the incubation medium prevents any reuptake of choline. In addition, since betaine, the oxidation product of choline is not extractable into tetraphenylboron - butyronitrile, no further procedure to eliminate choline is required.
Cooper
J R
JR
Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut 06510.
D000109:Acetylcholine / Q000032:analysis* / Q000378:metabolism; D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism*; D002794:Choline / Q000378:metabolism; D051381:Rats; D013574:Synaptosomes / Q000378:metabolism*; D014316:Tritium
D014316:Tritium; D000429:Alcohol Oxidoreductases; C019888:choline oxidase; D002794:Choline; D000109:Acetylcholine