Enhancement of proteinase-mediated degradation of proteins modified by chlorination.
1. Pretreatment of some proteins (albumin, immunoglobulin G, elastin and fibrinogen) with hypochlorite or with the MPO-H2O2-Cl- system increased their susceptibility to proteolysis by trypsin, chymotrypsin or elastase. 2. The optimal activities of these three proteinases were attained at a different extent of albumin chlorination. 3. Elastase was found to develop a specially efficient activity towards chlorinated albumin or chlorinated elastin being by itself resistant to chlorinating species.
Olszowska
E
E
Instytut Biochemii Lekarskiej Akademii Medycznej, Kraków, Poland.
Olszowski
S
S
Zgliczyński
J M
JM
Stelmaszyńska
T
T
D002713:Chlorine / Q000502:physiology*; D002918:Chymotrypsin / Q000378:metabolism; D004549:Elastin / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism*; D005340:Fibrinogen / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism; D010196:Pancreatic Elastase / Q000378:metabolism; D011479:Protamines / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013997:Time Factors; D014357:Trypsin / Q000378:metabolism
D007074:Immunoglobulin G; D011479:Protamines; D011506:Proteins; D012710:Serum Albumin, Bovine; D002713:Chlorine; D005340:Fibrinogen; D004549:Elastin; D010450:Endopeptidases; D002918:Chymotrypsin; D010196:Pancreatic Elastase; D014357:Trypsin