Doubling time of Mycobacterium lepraemurium in mouse footpads.
The doubling time of Mycobacterium lepraemurium (MLM) was measured in CBA/Ca mice. In eight experiments, 5 X 10(3) MLM were inoculated into the hind footpads of groups of mice, and the organisms were harvested from 1-45 days later. The harvested organisms were enumerated, and the doubling time was calculated assuming that MLM had multiplied without a lag phase and that multiplication continued at a constant rate from inoculation to harvest. Simultaneously, the proportions of viable organisms in the inocula were determined by inoculation of serially diluted suspensions into the footpads of other mice, harvesting 4 months later and calculating the most probable number. MLM were observed to multiply rapidly during the first several days, and more slowly thereafter; the mean initial doubling time was determined to be 0.5 days, a value much smaller than those previously reported by other workers.
Silbaq
F
F
Department of Comparative Medicine, Hebrew University-Hadassah Medical School, Jerusalem.
Levy
L
L
Bercovier
H
H
D000818:Animals; D005528:Foot; D051379:Mice; D008808:Mice, Inbred CBA / Q000276:immunology / Q000382:microbiology; D009167:Mycobacterium lepraemurium / Q000254:growth & development*