Toxicological evaluation of complex industrial wastes: implications for exposure assessment.
We evaluated a variety of short-term bioassays to construct a battery of tests that could be used for assessing the biological effects of potentially hazardous complex industrial wastes. Ten samples were studied for hepatotoxicity; these samples and an additional 5 were studied for mutagenicity. Although the data are limited to these samples, the results suggest that the Salmonella assay (strain TA98) or a prophage-induction assay (both in the presence of S9) in combination with determination of relative liver weight and levels of a set of serum enzymes in rats may provide a battery of tests suitable to characterize complex industrial wastes for mutagenic and hepatotoxic potential. The biological activities exhibited by the wastes were not readily predicted by the chemical profiles of the wastes, emphasizing the importance of characterizing potentially hazardous complex industrial wastes by both chemical and biological means. DNA from liver, lung and bladder of rats exposed to some of the wastes was analyzed by the 32P-postlabeling technique for the presence of DNA adducts. A waste that produced mutagenic urine produced a DNA adduct in bladder DNA. The implications of this approach for assessment of exposure to complex hazardous waste mixtures are discussed.
DeMarini
D M
DM
Health Effects Research Laboratory, U.S. Environmental Protection Agency, Research Triangle Park, NC 27711.
Gallagher
J E
JE
Houk
V S
VS
Simmons
J E
JE
D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000201:enzymology / Q000209:etiology; D004247:DNA / Q000032:analysis / Q000096:biosynthesis; D004781:Environmental Exposure; D004785:Environmental Pollutants / Q000633:toxicity*; D007220:Industrial Waste / Q000032:analysis*; D009152:Mutagenicity Tests; D051381:Rats; D011916:Rats, Inbred F344; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D001743:Urinary Bladder / Q000187:drug effects / Q000378:metabolism
D004785:Environmental Pollutants; D007220:Industrial Waste; D004247:DNA