[Serum levels of progesterone and estradiol in relation to tertiary follicles in the sheep ovary in proestrus and after administration of gonadoliberin].
Changes in progesterone (P4) and 17 beta-estradiol (E2) concentrations in blood serum as well as changes in counts and quality of tertiary follicles on ovary surfaces of ewes were investigated after administration of different GnRH doses on the 15th and 16th day of the oestral cycle. Progesterone (P4) and 17 beta-estradiol (E2) were determined by radioimmunological assays using RIA-test-Prog and RIA-test-Estra kits. It follows from the results that GnRH in Dirigestran inj. Spofa preparation reduces the E2 concentration and multiplication of the number of selected tertiary follicles of ovaries through stimulation of their growth and ripening in ewes of the Improved Wallachian breed. The greatest number of ovulations was reported in ewes administered three doses of the preparation. A positive correlation (r = 0.803) was found between the E2 concentration and the number of selected tertiary follicles of ewes in proestrus. The results corroborated the data on a pulsatile release of gonadoliberin during pre-ovulation incretion of gonadotropins--mainly lutropin (LH), as well as the inhibiting effect of gonadoliberin on the steroidogenesis of the ovary--mainly on estradiol production.
Maracek
I
I
Jusíková
A
A
Hendrichovský
V
V
Bekeová
E
E
Krajnicáková
M
M
D000818:Animals; D004958:Estradiol / Q000097:blood*; D004971:Estrus; D005260:Female; D006080:Ovarian Follicle / Q000187:drug effects / Q000502:physiology*; D010906:Pituitary Hormone-Releasing Hormones / Q000494:pharmacology*; D011359:Proestrus / Q000097:blood*; D011374:Progesterone / Q000097:blood*; D012756:Sheep / Q000097:blood*
D010906:Pituitary Hormone-Releasing Hormones; D011374:Progesterone; D004958:Estradiol
Koncentrácia progesterónu a estradiolu krvného séra vo vztahu k terciárnym folikulom vajecníka oviec v proestre a po podaní gonádoliberínu.