SpermCheck: a simplified screening assay for immunological infertility.
SpermCheck (Bio-Rad Laboratories, Hercules, CA), a new screening test for regional surface antibodies on motile sperm, uses monodispersed latex microspheres of uniform size as a vehicle to link rabbit antihuman immunoglobulins (IgA, IgG, IgM) and provides both negative and positive control sera, as well as sufficient buffer for sperm preparation in ambient CO2 atmosphere. When compared with reference data available for the immunobead test (IBT), the direct protocol (semen) for SpermCheck yielded 94.4% sensitivity with 100% specificity; the indirect protocol (serum) provided a sensitivity of 100% with 94.7% specificity. The microspheres of SpermCheck maintain a nearly uniform concentration per volume, with none to negligible clumping. The greater difference between the optical densities of latex and cytoplasm allows use of a light microscope for the rapid assessment of the percent of regional binding rather than the phase-contrast microscope required for the IBT. SpermCheck eliminates many difficulties encountered with the IBT, making SpermCheck a convenient screening assay for use in the physician's office.
McClure
R D
RD
Department of Urology, University of California School of Medicine, San Francisco 94143.
Tom
R A
RA
Watkins
M
M
Murthy
S
S
D000906:Antibodies / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007248:Infertility, Male / Q000175:diagnosis*; D007840:Latex; D008297:Male; D008863:Microspheres; D012680:Sensitivity and Specificity; D013094:Spermatozoa / Q000276:immunology*
D000906:Antibodies; D007840:Latex