[Immunological humoral response in mice experimentally infected with Echinococcus granulosus, preliminary result of kinetic observation].
5 serological methods included latex agglutination test (LA), enzyme-linked immunoelectrotransfer blot (EITB), enzyme-linked immunosorbent assay (ELISA), dot-enzyme-linked immunosorbent assay (Dot-ELISA) and immunofluorescence antibody test (IFA) were used to trace the kinetic changes of the humoral response in NIH mice experimentally infected with protoscolex of Echinococcus granulosus. Results showed that Dot-ELISA and IFA were able to detect antibodies 2 wk after infection while ELISA, EITB and LA gave positive reactions 4 wk, 2 mos and 3 mos after infection respectively. Therefore Dot-ELISA and IFA were considered to be the most sensitive ones among the 5 methods used. All infected mice gave positive reactions at 8 and 12 mos but none of the normal control mice showed any reaction throughout the period of observation (Fig. 1).
Xue
H C
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Qiu
L S
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Zhang
Y H
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Zhang
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Zhu
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Yang
Y Q
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Lu
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Li
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D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis*; D004443:Echinococcosis / Q000276:immunology*; D004446:Echinococcus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005455:Fluorescent Antibody Technique; D007841:Latex Fixation Tests; D051379:Mice
D000909:Antibodies, Helminth