[Photosystem II of rye. Nucleotide sequence of psbB and psbH genes, coding 47-kDa of chlorophyll(a)-binding and 10-kDa phosphorylated subunits].
Chloroplast DNA was isolated from rye seedlings by the non-aqueous method. The region of rye ctDNA which comprises two genes psbB and psbH encoding polypeptide subunits of photosystem II (47 kappa l) Chl alpha -binding protein (CP alpha -1) and 10 kD phosphoprotein, respectively) and two ORFs in the opposite strands in the psbB--psbH spacer region encoding hydrophobic peptides with strongly charged C-terminal segments was sequenced. The deduced amino acid sequences of polypeptide products of the genes were compared with those of different plant species (in case of the psbB product also with sequence of a cyano-bacterium Synechocystis) and revealed some highly conservative amino acid residues and regions of polypeptide chains, which apparently play essential role in the interaction with other PS II subunits and in the binding of chlorophyll molecules. Some speculations are made on the possible function of the peptides encoded by the two ORFs.
Bukharov
A A
AA
Kolosov
V L
VL
Zolotarev
A S
AS
Abdulaev
N G
NG
D000595:Amino Acid Sequence; D001483:Base Sequence; D002734:Chlorophyll / Q000235:genetics* / Q000378:metabolism; D002523:Edible Grain / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D045342:Light-Harvesting Protein Complexes; D008969:Molecular Sequence Data; D010766:Phosphorylation; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D015183:Restriction Mapping; D012434:Secale / Q000235:genetics* / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid
D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D002734:Chlorophyll
Fotosistema II rzhi. Nukleotidnaia posledovatel'nost' genov psbB i psbH, kodiruiushchikh 47-kDa khlorofill(a)-sviazyvaiushchuiu i 10-kDa fosforiliruiushchuiusia sub''edinitsy.