Interaction of tumor and surrounding tissue of mice inoculated B16 melanoma variants in terms of enzyme activity.
1. The interactions of B16-F1 and B16-F10 tumors with their surrounding tissues in terms of enzyme activities such as cathepsin B, hemoglobin(Hb)-hydrolase, acid phosphatase, beta-glucuronidase and plasminogen activator were investigated when said tumors proliferated locally and at secondary sites throughout the host's circulatory system. 2. In the case of B16-F1 and B16-F10 tumor cells proliferating under the skin, statistical differences were not detected between the enzyme activities of the skin surrounding the tumors and control skin, nor between B16-F1 and B16-F10 tumors, except for beta-glucuronidase. 3. In the case of B16-F1 and B16-F10 tumor cells metastasizing to lung, statistical differences were detected between numerous enzyme activities of the lung tissues surrounding the tumors and control lung tissue, and also between B16-F1 and B16-F10 tumors. 4. The activities of cathepsin B and acid phosphatase of lung tissue surrounding B16-F1 tumor were lower than those of the control lung. 5. beta-Glucuronidase activity of lung tissue surrounding B16-F10 tumor was higher than that of the control lung. 6. The activities of cathepsin B, Hb-hydrolase and beta-glucuronidase of the B16-F10 tumor were higher than those of the B16-F1 tumor. 7. Results indicate that metastasized B16 melanoma tumor cells interact with surrounding lung tissues, and that cathepsin B, Hb-hydrolase and beta-glucuronidase might play important roles in the metastasis of the malignant tumor.
Nakao
H
H
Department of Dermatology, Juntendo University, School of Medicine, Tokyo, Japan.
Takamori
K
K
Ogawa
H
H
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D002401:Cathepsin B / Q000378:metabolism; D004273:DNA, Neoplasm / Q000032:analysis; D005966:Glucuronidase / Q000378:metabolism; D008175:Lung Neoplasms / Q000556:secondary; D008546:Melanoma, Experimental / Q000201:enzymology* / Q000556:secondary; D051379:Mice; D010447:Peptide Hydrolases / Q000378:metabolism; D010960:Plasminogen Activators / Q000378:metabolism; D012878:Skin Neoplasms / Q000201:enzymology*
D004273:DNA, Neoplasm; D000135:Acid Phosphatase; D005966:Glucuronidase; D010447:Peptide Hydrolases; C022759:hemoglobin hydrolase; D010960:Plasminogen Activators; D002401:Cathepsin B