Analysis of the expression of two phosphoglycerate kinase genes in a mouse cultured cell line during activation and inactivation of the c-myc gene.
The effect of a change in the concentration of c-myc protein on the expression of genes for two phosphoglycerate kinase isozymes was investigated. The steady state levels of messenger ribonucleic acids (mRNAs) for sperm-type and non-sperm-type proteins were determined by blot hybridization using the RNA of the mouse cell line 38-2 containing the inducible rat c-myc gene cultured under various conditions. Without induction the c-myc gene. mRNA for non-sperm-type protein was detected at a level that remained essentially constant during both activation and inactivation of the c-myc gene. mRNA for sperm-type protein was not detected in 38-2 cells cultured under any conditions used. Change in the amount of c-myc protein alone does not appear to bring about a switch of the expression of the two phosphoglycerate kinase genes during spermatogenesis in mouse testis.
Koikeda
S
S
Tamaru
M
M
Goto
M
M
Mori
N
N
Takada
S
S
Obinata
M
M
Masamune
Y
Y
Nakanishi
Y
Y
D002460:Cell Line; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D010735:Phosphoglycerate Kinase / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics; D016271:Proto-Oncogene Proteins c-myc; D011519:Proto-Oncogenes
D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D004247:DNA; D010735:Phosphoglycerate Kinase