Detection of perforin and granzyme A mRNA in infiltrating cells during infection of mice with lymphocytic choriomeningitis virus.
The analysis of gene expression in cytotoxic T cells by in situ hybridization of serial liver and brain sections from mice infected with lymphocytic choriomeningitis virus (LCMV) and immunostaining with T cell marker- and virus-specific antibodies revealed a close histological association of infiltrating lymphocytes expressing the perforin and granzyme A genes with virally infected cells. Maximal frequency of perforin and granzyme A mRNA-containing cells on liver sections preceded by about 2 days maximal LCMV-specific cytotoxicity of the lymphoid liver infiltrating cells. These results are most consistent with an involvement of perforin and granzyme A in cell-mediated cytotoxicity in vivo.
Müller
C
C
Department of Pathology, University of Bern.
Kägi
D
D
Aebischer
T
T
Odermatt
B
B
Held
W
W
Podack
E R
ER
Zinkernagel
R M
RM
Hengartner
H
H
D000818:Animals; D001921:Brain / Q000382:microbiology / Q000503:physiopathology; D005786:Gene Expression Regulation; D053804:Granzymes; D006524:Hepatitis, Viral, Animal / Q000235:genetics; D007124:Immunoenzyme Techniques; D008099:Liver / Q000382:microbiology / Q000473:pathology / Q000503:physiopathology; D008216:Lymphocytic Choriomeningitis / Q000382:microbiology / Q000473:pathology / Q000503:physiopathology*; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D009693:Nucleic Acid Hybridization; D054353:Perforin; D052899:Pore Forming Cytotoxic Proteins; D015347:RNA Probes; D012333:RNA, Messenger / Q000235:genetics; D012697:Serine Endopeptidases / Q000235:genetics*; D013602:T-Lymphocytes, Cytotoxic / Q000502:physiology*; D013997:Time Factors
D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D052899:Pore Forming Cytotoxic Proteins; D015347:RNA Probes; D012333:RNA, Messenger; D054353:Perforin; D053804:Granzymes; D012697:Serine Endopeptidases