title: The C-terminal domain of the largest subunit of RNA polymerase II and transcription initiation.
abstract: Monoclonal antibodies specific for the evolutionarily conserved C-terminal heptapeptide repeat domain of the largest subunit of RNA polymerase II inhibited the initiation of transcription from mammalian promoters in vitro. Since these antibodies did not inhibit elongation and randomly initiated transcription, the heptapeptide repeats may function by binding class II transcription initiation factor(s).
authors.affiliation: Banting and Best Department of Medical Research, University of Toronto, Ontario, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Moyle
authors.forename: J S
authors.initials: JS
authors.lastname: Lee
authors.forename: W F
authors.initials: WF
authors.lastname: Anderson
authors.forename: C J
authors.initials: CJ
authors.lastname: Ingles
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D007700:Kinetics; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013950:Thymus Gland / Q000201:enzymology; D014158:Transcription, Genetic
chemical_list: D000911:Antibodies, Monoclonal; D046911:Macromolecular Substances; D012319:RNA Polymerase II