title: Determination of pipecolic acid in serum or plasma by solid-phase extraction and isotope dilution mass spectrometry.
abstract: The determination of pipecolic acid in serum or plasma by positive chemical ionization gas chromatography/mass spectrometry is assessed. This quantitative method involves stable isotope dilution and cation-exchange solid-phase extraction. Several derivatives of pipecolic acid and its octadeuterated analogue were investigated for their mass spectrometric characteristics. The heptafluorobutyric methyl ester derivatives afford optimal resolution on gas chromatography of biological extracts. Moreover, the derivatizing reagent (methanolic HCl) allows a combined elution and derivatization. Selected ion monitoring is performed on the [M + H]+ ions of both analyte and internal standard, at m/z 340 and 348, respectively. Serum or plasma samples from healthy subjects and patients suspected of peroxisomal diseases have been examined.
authors.affiliation: Laboratoria voor Medische Biochemie en Klinische Analyse, Rijksuniversiteit Gent, Belgium.
authors.forename: J F
authors.initials: JF
authors.lastname: Van Bocxlaer
authors.forename: B J
authors.initials: BJ
authors.lastname: Verhaeghe
authors.forename: A E
authors.initials: AE
authors.lastname: Wauters
authors.forename: W D
authors.initials: WD
authors.lastname: De Marez
authors.forename: L M
authors.initials: LM
authors.lastname: Thienpont
authors.forename: A P
authors.initials: AP
authors.lastname: De Leenheer
mesh_terms: D002648:Child; D002675:Child, Preschool; D002852:Chromatography, Ion Exchange; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007223:Infant; D013058:Mass Spectrometry; D008661:Metabolism, Inborn Errors / Q000097:blood; D008830:Microbodies / Q000378:metabolism; D010875:Pipecolic Acids / Q000097:blood*; D011865:Radioisotope Dilution Technique
chemical_list: D010875:Pipecolic Acids