title: Viruses with bisegmented double-stranded RNA in pig faeces.
abstract: Viruses similar to the bisegmented double-stranded (ds) RNA picobirnaviruses described in human faeces and the intestinal contents of Oryzomys nigripes rats and guinea pigs were isolated from the faeces of pigs taken from several areas in the state of Sao Paulo, Brazil. Samples were collected from 912 pigs of several breeds, aged nine to 61 days, and assayed by polyacrylamide gel electrophoresis with silver staining and a combined enzyme immunoassay for rotavirus and adenovirus, using the simian rotavirus SA11 as control. Electrophoretic profiles resembling the bisegmented dsRNA viruses were detected in 106 pigs with 15.3 per cent occurring in animals with diarrhoea compared to 9.6 per cent in animals without diarrhoea.
authors.affiliation: Department of Microbiology and Immunology, Campinas State University, Brazil.
authors.forename: M S
authors.initials: MS
authors.lastname: Gatti
authors.forename: A F
authors.initials: AF
authors.lastname: de Castro
authors.forename: M M
authors.initials: MM
authors.lastname: Ferraz
authors.forename: A M
authors.initials: AM
authors.lastname: Fialho
authors.forename: H G
authors.initials: HG
authors.lastname: Pereira
mesh_terms: D000818:Animals; D001938:Brazil; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000382:microbiology*; D007124:Immunoenzyme Techniques; D012328:RNA Viruses / Q000235:genetics / Q000302:isolation & purification*; D012330:RNA, Double-Stranded / Q000032:analysis*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
chemical_list: D012330:RNA, Double-Stranded