title: Development of insulin sensitivity in white adipose tissue during the suckling-weaning transition in the rat. Involvement of glucose transport and lipogenesis.
abstract: The changes of insulin responsiveness of white adipose tissue during the suckling-weaning transition in the rat were investigated in vitro on isolated adipocytes. Insulin binding, glucose transport and glucose metabolism in adipocytes from suckling rats and from rats weaned on to a high-carbohydrate (HC) or a high-fat (HF) diet were compared. Despite similar insulin binding, insulin-stimulated glucose transport rate is lower in adipocytes from suckling rats and HF-weaned rats than in adipocytes from HC-weaned rats. Moreover, whereas insulin markedly stimulates glucose metabolism in adipocytes from HC-weaned rats, glucose metabolism is totally unresponsive to insulin in adipocytes from suckling and HF-weaned rats. This insulin resistance is associated with a very low rate of lipogenesis and low activities of acetyl-CoA carboxylase, fatty acid synthase and pyruvate dehydrogenase.
authors.affiliation: Centre de Recherche sur la Nutrition (CNRS), Meudon-Bellevue, France.
authors.forename: T
authors.initials: T
authors.lastname: Issad
authors.forename: P
authors.initials: P
authors.lastname: Ferré
authors.forename: M
authors.initials: M
authors.lastname: Pastor-Anglada
authors.forename: M A
authors.initials: MA
authors.lastname: Baudon
authors.forename: J
authors.initials: J
authors.lastname: Girard
mesh_terms: D000273:Adipose Tissue / Q000502:physiology*; D000818:Animals; D000833:Animals, Suckling / Q000502:physiology*; D004041:Dietary Fats / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism / Q000494:pharmacology*; D007333:Insulin Resistance; D008055:Lipids / Q000096:biosynthesis; D009004:Monosaccharide Transport Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014886:Weaning
chemical_list: D004041:Dietary Fats; D007328:Insulin; D008055:Lipids; D009004:Monosaccharide Transport Proteins; D005947:Glucose