title: Extrachromosomal elements cause a reduced division potential in nib 1 strains of Saccharomyces cerevisiae.
abstract: The nib 1 allele of yeast confers a sensitivity to an endogenous plasmid, 2 mu DNA, in that nib 1 strains bearing 2 mu DNA (cir+) exhibit a reduction in division potential. In the present study, the reduction in division potential characteristic of nib 1 cir+ strains is shown to be dependent on the simultaneous presence of both the A and the D open reading frames of 2 mu DNA as well as on the presence of an unidentified extrachromosomal element other than 2 mu DNA. Furthermore, in nib 1 strains, an uncharacterized extrachromosomal element can cause a less severe reduction of division potential in the absence of intact 2 mu DNA. Thus, the nib 1 allele may confer a generalized sensitivity to extrachromosomal elements.
authors.affiliation: Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
authors.forename: R
authors.initials: R
authors.lastname: Sweeney
authors.forename: V A
authors.initials: VA
authors.lastname: Zakian
mesh_terms: D000483:Alleles; D015139:Blotting, Southern; D002455:Cell Division; D003433:Crosses, Genetic; D004271:DNA, Fungal / Q000235:genetics*; D004171:Diploidy; D005800:Genes, Fungal; D005838:Genotype; D009154:Mutation; D010641:Phenotype; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics*; D014170:Transformation, Genetic
chemical_list: D004271:DNA, Fungal