Direct expression cloning of vascular cell adhesion molecule 1, a cytokine-induced endothelial protein that binds to lymphocytes.
We have cloned a previously undescribed adhesion molecule, VCAM-1, which is induced by cytokines on human endothelial cells and binds lymphocytes. Using a novel method requiring neither monoclonal antibodies nor purified protein, VCAM-1-expressing clones were selected by adhesion to human lymphoid cell lines. VCAM-1 mRNA is present in endothelial cells at 2 hr after treatment with IL-1 or TNF-alpha and is maintained for at least 72 hr; leukocyte binding activity parallels mRNA induction. Cells transfected with VCAM-1 bind the human leukemia lines Jurkat, Ramos, Raji, HL60, and THP1, but not peripheral blood neutrophils. VCAM-1, which belongs to the immunoglobulin gene super-family, may be central to recruitment of mononuclear leukocytes into inflammatory sites in vivo.
Osborn
L
L
Biogen, Inc., Cambridge Center, Massachusetts 02142.
Hession
C
C
Tizard
R
R
Vassallo
C
C
Luhowskyj
S
S
Chi-Rosso
G
G
Lobb
R
R
D000595:Amino Acid Sequence; D001483:Base Sequence; D001685:Biological Factors / Q000494:pharmacology*; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D002478:Cells, Cultured; D003001:Cloning, Molecular; D016207:Cytokines; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects*; D015723:Gene Library; D005796:Genes; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D008214:Lymphocytes / Q000378:metabolism*; D008969:Molecular Sequence Data; D011485:Protein Binding; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D001685:Biological Factors; D015815:Cell Adhesion Molecules; D016207:Cytokines; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D004247:DNA