The Saccharomyces cerevisiae SPT13/GAL11 gene has both positive and negative regulatory roles in transcription.
To understand the function of SPT13, a gene encoding a trans-acting factor involved in regulation of Ty-mediated gene expression, we have cloned and sequenced the gene. Our analysis revealed that SPT13 is the same gene as GAL11, a gene in which mutations cause reduced expression of some GAL4-regulated genes. Further analysis of spt13/gal11 mutants suggested that the SPT13 gene also affects transcription of genes involved in mating-type specialization. We show here that SPT13 has both positive and negative regulatory roles in transcription.
Fassler
J S
JS
Department of Biology, University of Iowa, Iowa City 52242.
Winston
F
F
D015152:Blotting, Northern; D003001:Cloning, Molecular / Q000379:methods; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005809:Genes, Regulator; D005838:Genotype; D000072235:Mating Factor; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010455:Peptides / Q000235:genetics; D010957:Plasmids; D012331:RNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics*; D014158:Transcription, Genetic
D010455:Peptides; D012331:RNA, Fungal; D015534:Trans-Activators; D000072235:Mating Factor