Cloning and expression of the thermostable alpha-amylase gene from Clostridium thermosulfurogenes (DSM 3896) in Escherichia coli.
The gene coding for a thermostable alpha-amylase from Clostridium thermosulfurogenes (DSM 3896) was cloned in Escherichia coli using pUC18 as a vector. The recombinant plasmid pCT2 of an amylolytic positive transformant of E. coli contained a 2.9 kbp fragment of chromosomal DNA of C. thermosulfurogenes carrying the alpha-amylase gene. In E. coli the gene was apparently transcribed by its own promoter. Comparative studies showed no difference between the original and the heterologously in E. coli expressed enzyme. The latter was not secreted into the medium.
Haeckel
K
K
Institut für Mikrobiologie, Georg-August-Universität, Göttingen, F.R.G.
Bahl
H
H
D003001:Cloning, Molecular; D003013:Clostridium / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D006358:Hot Temperature; D010957:Plasmids; D015183:Restriction Mapping; D014169:Transformation, Bacterial; D000516:alpha-Amylases / Q000235:genetics* / Q000378:metabolism
D004269:DNA, Bacterial; D000516:alpha-Amylases