Neutrophil activating factor (NAF) induces polymorphonuclear leukocyte adherence to endothelial cells and to subendothelial matrix proteins.
Neutrophil activating factor is a polypeptide cytokine released from stimulated mononuclear phagocytes and endothelial cells. We found that neutrophil activating factor induced time- and concentration-dependent binding of human polymorphonuclear leukocytes to endothelial monolayers and subendothelial matrix proteins, via a mechanism that involves altered expression of the leukocyte CD11/CD18 glycoproteins. Thus, neutrophil activating factor is a third mediator, in addition to platelet-activating factor and endothelial leukocyte adhesion molecule 1, that is synthesized by activated endothelium and that can induce polymorphonuclear leukocyte adhesion to endothelial cells. Because NAF is released into the pericellular fluid, it may also stimulate binding of the leukocytes to exposed subendothelial structures at sites of vascular injury.
Carveth
H J
HJ
Nora Eccles Harrison Cardiovascular Research and Training Institute, University of Utah, Salt Lake City.
Bohnsack
J F
JF
McIntyre
T M
TM
Baggiolini
M
M
Prescott
S M
SM
Zimmerman
G A
GA
D000954:Antigens, Surface / Q000502:physiology; D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000032:analysis / Q000502:physiology*; D005109:Extracellular Matrix / Q000032:analysis / Q000502:physiology*; D006801:Humans; D016023:Integrins; D016209:Interleukin-8; D007700:Kinetics; D008562:Membrane Glycoproteins / Q000502:physiology; D009504:Neutrophils / Q000502:physiology*; D010455:Peptides / Q000494:pharmacology*; D010972:Platelet Activating Factor / Q000502:physiology; D013347:Subcellular Fractions / Q000502:physiology
D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D016023:Integrins; D016209:Interleukin-8; D008562:Membrane Glycoproteins; D010455:Peptides; D010972:Platelet Activating Factor