Design of RNA enzymes for sequence-dependent cleavage of RNA.
We have designed synthetic ribozymes which can cleave specific sites of RNA. An undecamer, 5S rRNA and transcripts using T7 RNA polymerase were hydrolyzed at the X position of RNA containing (N)nUX(N)n (N = A, G, C and U; X = A, C and U) by these ribozymes.
Koizumi
M
M
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Hayase
Y
Y
Iwai
S
S
Kamiya
H
H
Inoue
H
H
Ohtsuka
E
E
D001483:Base Sequence; D002384:Catalysis; D008969:Molecular Sequence Data; D009843:Oligoribonucleotides / Q000138:chemical synthesis / Q000378:metabolism*; D016337:RNA, Catalytic; D012335:RNA, Ribosomal / Q000378:metabolism*
D009843:Oligoribonucleotides; D016337:RNA, Catalytic; D012335:RNA, Ribosomal