title: Crystallization of alcohol oxidase from Pichia pastoris.
abstract: Crystals of alcohol oxidase purified from Pichia pastoris were grown in microdialysis buttons in a solution of polyethylene glycol, sodium chloride and sodium azide. The crystals were stratified along the major axis and up to 3 mm in length. X-ray diffraction experiments indicated a space group of P2(1) and unit cell dimensions of a = 157.3 A, b = 171.5 A and c = 231.6 A. Crystals diffract to beyond 2.7 A and are suitable for X-ray structure analysis.
authors.affiliation: Astbury Department of Biophysics, University of Leeds, U.K.
authors.forename: C W
authors.initials: CW
authors.lastname: Boys
authors.forename: D J
authors.initials: DJ
authors.lastname: Hill
authors.forename: P G
authors.initials: PG
authors.lastname: Stockley
authors.forename: J R
authors.initials: JR
authors.lastname: Woodward
mesh_terms: D000429:Alcohol Oxidoreductases; D003460:Crystallization; D010843:Pichia / Q000201:enzymology*; D004718:Saccharomycetales / Q000201:enzymology*; D014961:X-Ray Diffraction
chemical_list: D000429:Alcohol Oxidoreductases; C019643:alcohol oxidase