An enzyme immunoassay for the determination of low levels of IgA in human serum.
An enzyme immunoassay (EIA) has been developed which facilitates the detection of low levels of immunoglobulin A in human serum. IgA is captured by an anti-IgA antibody linked to micron-sized polyacrylamide beads and subsequently detected by an anti-IgA horseradish peroxidase conjugate. The standard curve is linear in the region between 25-1000 ng/ml IgA. The assay is particularly suited to measure IgA antibodies in sera from IgA-deficient individuals and IgA contaminants in blood products.
Haun
M
M
Protein Chemistry Section, Canadian Red Cross Society, Ottawa, Ontario.
Wasi
S
S
D000818:Animals; D006801:Humans; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A / Q000032:analysis* / Q000276:immunology; D011817:Rabbits; D011863:Radioimmunoassay
D007070:Immunoglobulin A