The thymus has two functionally distinct populations of immature alpha beta + T cells: one population is deleted by ligation of alpha beta TCR.
During T cell development, the processes of selection and tolerance act on the universe of expressed T cell receptors in the thymic cortex to form the repertoire of mature T cells that will respond to foreign antigen in the context of self-MHC in that animal. We have subdivided the cortical thymocytes into three functionally distinct populations: one population which is antigen-receptor negative, a second population which is antigen-receptor positive and is resistant to deletion by signaling through the antigen receptor, and a third population which is also antigen-receptor positive but is sensitive to deletion. These results have implications for the cellular compartments in which positive and negative selection occur and for the biochemical mechanisms that mediate selection and tolerance.
Finkel
T H
TH
Department of Pediatrics, Howard Hughes Medical Institute, Denver, Colorado 80206.
Cambier
J C
JC
Kubo
R T
RT
Born
W K
WK
Marrack
P
P
Kappler
J W
JW
D000818:Animals; D002118:Calcium / Q000378:metabolism; D005333:Fetus; D005455:Fluorescent Antibody Technique; D008285:Major Histocompatibility Complex; D051379:Mice; D009924:Organ Culture Techniques; D011948:Receptors, Antigen, T-Cell / Q000032:analysis* / Q000235:genetics; D013601:T-Lymphocytes / Q000145:classification* / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology*
D011948:Receptors, Antigen, T-Cell; D002118:Calcium