title: Production and evaluation of recombinant hirudin.
abstract: Hirudin, a 65 amino acid polypeptide form the medicinal leech, is an extremely efficient and specific thrombin inhibitor whose therapeutic potential has been demonstrated in a number of animal models. We have developed protocols for the production of recombinant hirudin by secretion from S. cerevisiae and carried out a full biologic evaluation of the purified product. These studies showed that natural and recombinant hirudin was similar in structure and in biologic function in vitro. Moreover, the recombinant protein displayed strong antithrombotic activity in several experimental thrombosis models in vivo, confirming the molecule's promise in the therapy of thrombotic disorders.
authors.affiliation: Transgene S.A., Strasbourg, France.
authors.forename: M
authors.initials: M
authors.lastname: Courtney
authors.forename: G
authors.initials: G
authors.lastname: Loison
authors.forename: Y
authors.initials: Y
authors.lastname: Lemoine
authors.forename: N
authors.initials: N
authors.lastname: Riehl-Bellon
authors.forename: E
authors.initials: E
authors.lastname: Degryse
authors.forename: S W
authors.initials: SW
authors.lastname: Brown
authors.forename: J P
authors.initials: JP
authors.lastname: Cazenave
authors.forename: G
authors.initials: G
authors.lastname: Defreyn
authors.forename: D
authors.initials: D
authors.lastname: Delebassee
authors.forename: A
authors.initials: A
authors.lastname: Bernat
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D024764:Hirudin Therapy; D006629:Hirudins / Q000494:pharmacology; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology / Q000627:therapeutic use; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013927:Thrombosis / Q000517:prevention & control
chemical_list: D006629:Hirudins; D011994:Recombinant Proteins