Humoral immune responses after cardiac transplantation: correlation with fatal rejection and graft atherosclerosis.
Although the advent of cyclosporine has allowed dramatic improvement in survival rates after heart transplantation, long-term outcome remains limited by rejection and graft atherosclerosis. We have previously demonstrated the development of alloreactive lymphocytotoxic antibodies in baboon recipients of heterotopic cardiac transplants despite cyclosporine administration. The hypothesis of our study is that human heart transplant recipients given treatment with cyclosporine are also capable of generating strong humoral immune responses that might adversely affect clinical outcome. Serial serum specimens from 118 heart transplant recipients were tested against a reference panel of 70 cells for anti-HLA lymphocytotoxic antibodies. Patients with positive sera on at least three separate samplings at minimal intervals of 1 week were considered to be antibody producers (Ab+), and those with less than three positive sera samplings were considered nonproducers (Ab-). Donor lymphocytes were not available for most recipients for the assessment of the specificity of antibodies produced. Seventy-six of 118 patients (64%) were Ab+. One-year, 3-year, and 5-year actuarial survival rates were 81%, 70%, and 53%, respectively, for Ab+ patients compared with corresponding rates of 93%, 90%, and 90%, respectively, in Ab- patients (p less than 0.01). Graft atherosclerosis confirmed by coronary angiography or autopsy developed in 12 Ab+ patients (16%), compared with 1 of 42 Ab- patients (2.3%) (p less than 0.05). These data show that almost two thirds of heart transplant recipients produce anti-HLA antibodies after grafting that correlate strongly with adverse outcome. Immunotherapies directed at control of deleterious humoral immune responses need to be developed.
Rose
E A
EA
Department of Surgery, Columbia University, College of Physicians and Surgeons, New York, N.Y.
Smith
C R
CR
Petrossian
G A
GA
Barr
M L
ML
Reemtsma
K
K
D000206:Actuarial Analysis; D000293:Adolescent; D000328:Adult; D000906:Antibodies / Q000276:immunology; D000917:Antibody Formation; D002648:Child; D002675:Child, Preschool; D003324:Coronary Artery Disease / Q000209:etiology*; D006084:Graft Rejection; D006680:HLA Antigens / Q000276:immunology; D016027:Heart Transplantation; D006801:Humans; D008875:Middle Aged; D009026:Mortality; D011184:Postoperative Period
D000906:Antibodies; D006680:HLA Antigens