[The level of cytochrome P-450 in Saccharomyces cerevisiae with disruptions of various stages of sterol synthesis].
A spectral analysis of cytochromes P-450 in Saccharomyces cerevisiae cells and in mutant strains accumulating the ergosterol biosynthesis intermediates was carried out. Glucose repression and semianaerobiosis were found to induce cytochrome P-450 synthesis. No differences in the cytochrome P-450 content in mutant nys 3, nys 4 and parent strains were observed. Mutants nys 5 accumulated large amounts of cytochrome P-450. Cytochrome P-420 was detected in wild type strains and in mutants nys 3 and nys 4. The cultivation time and aeration conditions were shown to be unimportant for the generation of cytochrome P-420.
Ekhvalova
T V
TV
Sharyshev
A A
AA
Mikhaĭlova
N P
NP
V'iunov
K A
KA
D003577:Cytochrome P-450 Enzyme System / Q000032:analysis* / Q000096:biosynthesis; D004790:Enzyme Induction; D005800:Genes, Fungal; D009154:Mutation; D010100:Oxygen / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D013261:Sterols / Q000096:biosynthesis*; D013997:Time Factors
D013261:Sterols; D003577:Cytochrome P-450 Enzyme System; D010100:Oxygen
Soderzhanie tsitokhroma P-450 v drozhzhakh Saccharomyces cerevisiae s narusheniiami razlichnykh stadiĭ sterinovogo sinteza.