title: Subcellular distribution and immunocytochemical localization of protein kinase C in myocardium, and phosphorylation of troponin in isolated myocytes stimulated by isoproterenol or phorbol ester.
abstract: Protein kinase C (PKC) catalytic activity was found in the cytosol, sarcolemma and sarcoplasmic reticulum, and PKC immunoreactivity was found in the striated regions and sarcolemma of rat hearts. Enhanced phosphorylation of troponin T and, to a lesser extent, troponin I was noted in isolated rat cardiac myocytes incubated with PKC activator phorbol ester, but only the phosphorylation of troponin I was stimulated by isoproterenol. It is suggested that PKC-mediated phosphorylation of troponin might be involved in regulation of myocardial function or in pathophysiology of the heart.
authors.affiliation: Department of Pharmacology, Emory University School of Medicine, Atlanta, Georgia 30322.
authors.forename: J D
authors.initials: JD
authors.lastname: Liu
authors.forename: J G
authors.initials: JG
authors.lastname: Wood
authors.forename: R L
authors.initials: RL
authors.lastname: Raynor
authors.forename: Y C
authors.initials: YC
authors.lastname: Wang
authors.forename: T A
authors.initials: TA
authors.lastname: Noland
authors.forename: A A
authors.initials: AA
authors.lastname: Ansari
authors.forename: J F
authors.initials: JF
authors.lastname: Kuo
mesh_terms: D000818:Animals; D002451:Cell Compartmentation; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology*; D009206:Myocardium / Q000201:enzymology*; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014336:Troponin / Q000378:metabolism*
chemical_list: D010750:Phosphoproteins; D014336:Troponin; D011493:Protein Kinase C; D007545:Isoproterenol; D013755:Tetradecanoylphorbol Acetate