Potentiation of radiation-induced bacterial cell killing by binuclear rhodium(II) carboxylates.
A series of binuclear rhodium(II) tetracarboxylate complexes was examined for potentiation of radiation-induced killing of Salmonella typhimurium cells. Carboxylate bridging ligands were varied as formate, acetate, trifluoroacetate, and propionate. All complexes caused hypoxic non-dose-modifying radiation potentiation in that variable thresholds were obtained with the radiation dose response. In phosphate-buffered saline (PBS), decreasing threshold doses, i.e., increasing potentiating efficiencies, were seen in the order of acetate = trifluoroacetate less than propionate less than formate. Beyond the threshold dose, the degree of potentiation for all complexes in PBS approximated 12 times the degree of radiation sensitivity seen for the N2 baseline of the radiation dose-response curve. No radiation potentiation by Rh2 carboxylates was seen for fully oxic suspensions. Irradiation of cells in the absence of phosphate increased the efficiency as well as the degree of radiation potentiation. It is hypothesized that bacterial radiation potentiation is initiated by one-electron reduction of the Rh2 carboxylates, most likely involving the hydrated electron, followed then by formation of an active product. These events likely occur outside the bacterial cell.
Richmond
R C
RC
Department of Radiation Oncology, Dartmouth-Hitchcock Medical Center, Hanover, New Hampshire 03756.
Farrell
N P
NP
Mahtani
H K
HK
D001419:Bacteria / Q000187:drug effects / Q000528:radiation effects*; D002264:Carboxylic Acids / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D009942:Organometallic Compounds / Q000494:pharmacology*; D010100:Oxygen / Q000502:physiology; D012238:Rhodium / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000187:drug effects / Q000528:radiation effects
D002264:Carboxylic Acids; D009942:Organometallic Compounds; D012238:Rhodium; D010100:Oxygen