Purification and properties of the very high density lipoprotein from the hemolymph of adult Triatoma infestans.
The very high density lipoprotein (VHDL) of Triatoma infestans hemolymph from adult males has been isolated and purified by two-step density gradient ultracentrifugation. It appears to be homogeneous as judged by native polyacrylamide gel electrophoresis. The content of VHDL in hemolymph was estimated to be 8 mg protein/ml. The purified protein has a molecular weight (Mr) of 450,000, is composed of six subunits of Mr approximately equal to 77,000, and possesses a high content of aromatic amino acids. This protein is glycosylated and contains 3% of lipids by weight with a remarkable amount of free fatty acids (25% of total lipids). The T. infestans VHDL has a different lipid and amino acid composition from lipophorin. The lipid composition and the spectroscopic studies using cis-parinaric acid indicated a high fatty acid binding affinity. It has nine binding sites per mol of VHDL. Competence studies revealed that VHDL has its highest affinity for the binding of palmitic acid followed by stearic and arachidonic acids.
Rimoldi
O J
OJ
Instituto de Investigaciones Bioquímicas de La Plata, CONICET-UNLP, Facultad de Cs. Médicas, Argentina.
Soulages
J L
JL
González
S M
SM
Peluffo
R O
RO
Brenner
R R
RR
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005227:Fatty Acids / Q000032:analysis; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D005453:Fluorescence; D006031:Glycosylation; D006458:Hemolymph / Q000032:analysis*; D008055:Lipids / Q000032:analysis; D008075:Lipoproteins, HDL / Q000032:analysis / Q000302:isolation & purification*; D008970:Molecular Weight; D014224:Triatoma / Q000032:analysis*; D014225:Triatominae / Q000032:analysis*
D000596:Amino Acids; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D008055:Lipids; D008075:Lipoproteins, HDL; C013641:parinaric acid