Comparison of R- and S-form lipopolysaccharides fractionated from Escherichia coli UKT-B lipopolysaccharide in pyrogen and Limulus tests.
Different LPS was shown to have a relatively different proportion of O-specific chain-less (R-form) LPS by polyacrylamide gel electrophoresis (PAGE) with sodium deoxycholate (DOC). By using DOC-PAGE, S-form LPS having O-chain with approximately 11 repeating units on average (S-Fr) and O-chain-less LPS (R-Fr) were separated from Escherichia coli UKT-B S-form LPS. Significantly stronger pyrogenicity was observed in R-Fr than in S-Fr when measured on the weight basis. Similar result was observed in Limulus test. Comparing biological activities of different S-form LPS, attention should be given to the amounts of co-existing R-form LPS.
Komuro
T
T
National Institute of Hygienic Sciences, Osaka Branch, Japan.
Yomota
C
C
Kimura
T
T
Galanos
C
C
D003840:Deoxycholic Acid; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004926:Escherichia coli / Q000378:metabolism; D008033:Limulus Test; D008070:Lipopolysaccharides / Q000378:metabolism*; D015394:Molecular Structure; D011749:Pyrogens
D008070:Lipopolysaccharides; D011749:Pyrogens; D003840:Deoxycholic Acid