Detection and characterization of IgE-producing cells in patients with clonorchiasis.
The ability of peripheral B cells of patients with Clonorchis sinensis infections to secrete IgE spontaneously was investigated in vitro. The de novo synthesis of IgE was observed in unstimulated B-cell cultures of patients. There was a significant relationship between the serum IgE level and the amount of IgE spontaneously secreted by B cells. Pretreatment of patients' B cells with 10 micrograms/ml of rabbit anti-human IgE resulted in the clear suppression of spontaneous IgE synthesis without affecting the IgG synthesis. Their B cells capable of spontaneously secreting IgE were partially sensitive to irradiation with 1,000 rad. The results obtained suggest that such IgE-forming cells may be responsible for at least part of the persistent IgE formation in patients with helminthic infections as well as in those with atopic disease.
Yanagihara
Y
Y
Clinical Research Center for Allergy, National Sagamihara Hospital, Kanagawa, Japan.
Kiniwa
M
M
Kajiwara
K
K
Joh
K
K
Kobayashi
A
A
Watanabe
N
N
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis*; D000917:Antibody Formation / Q000528:radiation effects; D000921:Antibody-Producing Cells / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology* / Q000528:radiation effects; D003003:Clonorchiasis / Q000276:immunology*; D003004:Clonorchis sinensis / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000096:biosynthesis; D066298:In Vitro Techniques
D000909:Antibodies, Helminth; D007074:Immunoglobulin G; D007073:Immunoglobulin E