High-performance liquid chromatographic monitoring of transpeptidation reactions in analogues of gonadotropin releasing hormone containing aspartic acid derivatives in position six.
High-performance liquid chromatographic systems were used for monitoring the hydrolysis of five potent agonistic gonadotropin releasing hormone analogues containing aspartic acid derivatives in position six. To separate the closely related nonapeptides formed during the hydrolysis, columns with reversed-phase packings were used under isocratic conditions. The mobile phases were methanol containing ammonium acetate or triethylammonium phosphate buffers. Good separations of the hydrolysis products from the investigated peptides allowed the reaction rate constants for the transformations examined to be calculated.
Vadász
Z
Z
First Institute of Biochemistry, Semmelweis University Medical School, Budapest, Hungary.
Seprödi
J
J
Erchegyi
J
J
Teplán
I
I
Schön
I
I
D000595:Amino Acid Sequence; D001224:Aspartic Acid / Q000378:metabolism*; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008969:Molecular Sequence Data; D010906:Pituitary Hormone-Releasing Hormones / Q000378:metabolism*; D011487:Protein Conformation; D013056:Spectrophotometry, Ultraviolet
D010906:Pituitary Hormone-Releasing Hormones; D001224:Aspartic Acid