title: Purification and identification of a neutral endopeptidase in Plasmodium falciparum schizonts and merozoites.
abstract: An endopeptidase specific to the Plasmodium falciparum erythrocytic schizont stage and to free merozoites was detected using the fluorogenic GlcA-Val-Leu-Gly-Lys(or Arg)-AEC substrate. The enzyme was purified by high performance liquid chromatography (HPLC); its optimal activity was around pH 7.5 and its isoelectric point was 4.4. The molecular weight of the enzyme was about 68,000, as demonstrated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions. The endopeptidase was strongly inhibited by thiol proteinase inhibitors, leupeptin, and antipain. The possible involvement of this neutral endopeptidase in the reinvasion process is discussed.
authors.affiliation: Laboratoire de Biologie Cellulaire, URA CNRS no 290, Université de Poitiers, France.
authors.forename: P
authors.initials: P
authors.lastname: Grellier
authors.forename: I
authors.initials: I
authors.lastname: Picard
authors.forename: F
authors.initials: F
authors.lastname: Bernard
authors.forename: R
authors.initials: R
authors.lastname: Mayer
authors.forename: H G
authors.initials: HG
authors.lastname: Heidrich
authors.forename: M
authors.initials: M
authors.lastname: Monsigny
authors.forename: J
authors.initials: J
authors.lastname: Schrével
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000032:analysis / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D007526:Isoelectric Point; D008970:Molecular Weight; D010963:Plasmodium falciparum / Q000201:enzymology*
chemical_list: D010450:Endopeptidases