title: Stabilization of phage T4 lysozyme by engineered disulfide bonds.
abstract: Four different disulfide bridges (linking positions 9-164, 21-142, 90-122, and 127-154) were introduced into a cysteine-free phage T4 lysozyme at sites suggested by theoretical calculations and computer modeling. The new cysteines spontaneously formed disulfide bonds on exposure to air in vitro. In all cases the oxidized (crosslinked) lysozyme was more stable than the corresponding reduced (noncrosslinked) enzyme toward thermal denaturation. Relative to wild-type lysozyme, the melting temperatures of the 9-164 and 21-142 disulfide mutants were increased by 6.4 degrees C and 11.0 degrees C, whereas the other two mutants were either less stable or equally stable. Measurement of the equilibrium constants for the reduction of the engineered disulfide bonds by dithiothreitol indicates that the less thermostable mutants tend to have a less favorable crosslink in the native structure. The two disulfide bridges that are most effective in increasing the stability of T4 lysozyme have, in common, a large loop size and a location that includes a flexible part of the molecule. The results suggest that stabilization due to the effect of the crosslink on the entropy of the unfolded polypeptide is offset by the strain energy associated with formation of the disulfide bond in the folded protein. The design of disulfide bridges is discussed in terms of protein flexibility.
authors.affiliation: Institute of Molecular Biology, University of Oregon, Eugene 97403.
authors.forename: M
authors.initials: M
authors.lastname: Matsumura
authors.forename: W J
authors.initials: WJ
authors.lastname: Becktel
authors.forename: M
authors.initials: M
authors.lastname: Levitt
authors.forename: B W
authors.initials: BW
authors.lastname: Matthews
mesh_terms: D004220:Disulfides; D004795:Enzyme Stability; D004926:Escherichia coli / Q000201:enzymology; D005818:Genetic Engineering; D014644:Genetic Variation; D009113:Muramidase / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D011487:Protein Conformation; D013604:T-Phages / Q000201:enzymology* / Q000235:genetics; D013816:Thermodynamics
chemical_list: D004220:Disulfides; D009113:Muramidase