The human protooncogene product p33pim is expressed during fetal hematopoiesis and in diverse leukemias.
We measured the human pim-1 protooncogene (PIM) expression during fetal development and in hematopoietic malignancies. Our data indicate that during human fetal hematopoiesis the 33-kDa pim product, p33pim, is highly expressed in the liver and spleen. In contrast, at the adult stage it is only slightly expressed in circulating granulocytes. Out of 70 hematopoietic malignancies analyzed, 51 patients and 19 cell lines, p33pim was overexpressed in approximately 30% of the samples, particularly in myeloid and lymphoid acute leukemias. This overexpression was unrelated to any stage of cellular differentiation and was not due to gene rearrangement or amplification. These results imply a physiological role of the pim-1 protooncogene during hematopoietic development and a deregulation in various leukemias.
Amson
R
R
Institute of Interdisciplinary Research, School of Medicine, Free University of Brussels, Belgium.
Sigaux
F
F
Przedborski
S
S
Flandrin
G
G
Givol
D
D
Telerman
A
A
D000328:Adult; D000911:Antibodies, Monoclonal; D015139:Blotting, Southern; D015153:Blotting, Western; D002460:Cell Line; D005333:Fetus / Q000502:physiology*; D015870:Gene Expression; D006410:Hematopoiesis; D006801:Humans; D007938:Leukemia / Q000201:enzymology / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000235:genetics*; D051573:Proto-Oncogene Proteins c-pim-1; D011519:Proto-Oncogenes
D000911:Antibodies, Monoclonal; D011518:Proto-Oncogene Proteins; D011505:Protein-Tyrosine Kinases; C496634:PIM1 protein, human; D017346:Protein Serine-Threonine Kinases; D051573:Proto-Oncogene Proteins c-pim-1