Structural similarity of the type-specific group B streptococcal polysaccharides and the carbohydrate units of tissue glycoproteins: evaluation of possible cross-reactivity.
Type-specific capsular polysaccharides of group B streptococci show striking structural similarity with the terminal sugar sequences of tissue glycoconjugates. The polysaccharides have been put forward as vaccines against neonatal meningitis. A potential source of hazard in immunization of pregnant mothers may be the presence of the cross-reactive components in adult or fetal tissues. A radioactive ligand binding assay was used to test human immune sera to type Ia, II and III group B streptococcal polysaccharides for binding to tissue-derived glycopeptides showing structural similarities with the streptococcal polysaccharides. Of the 13 glycopeptides of human and rat tissues studied, representing a wide selection of structures known to occur in glycoproteins, only two showed some reactivity with the antisera. The reactivity with human small intestinal glycopeptides could be explained by the presence of natural blood group A antibodies, and was not related to the streptococcal group B antibodies. The basis of the reactivity of a high-molecular-weight glycopeptide from rat kidney with some of the sera was unknown, but was unrelated to the vaccination and clearly could not be inhibited with the streptococcal polysaccharides. Thus, no immunological cross-reactions of the tissue glycopeptides studied could be demonstrated with the group B streptococcal antisera.
Häyrinen
J
J
Department of Biochemistry, University of Kuopio, Finland.
Pelkonen
S
S
Finne
J
J
D000017:ABO Blood-Group System; D000818:Animals; D000937:Antigen-Antibody Reactions; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D015394:Molecular Structure; D011135:Polysaccharides, Bacterial / Q000276:immunology; D011485:Protein Binding; D051381:Rats; D013292:Streptococcus agalactiae / Q000276:immunology*
D000017:ABO Blood-Group System; D006023:Glycoproteins; D007106:Immune Sera; D011135:Polysaccharides, Bacterial