An antifibrin monoclonal antibody useful in immunoscintigraphic detection of thrombi.
Balb/c mice were immunized with human plasmin-generated fibrinogen degradation product Y. Spleen cells were fused with P3X63-Ag8.653 myeloma cells. A clone (Y22) was found that produces monoclonal antibodies (MoAbs) with a strong reactivity with human fibrin and only a weak reactivity with fibrinogen in an enzyme immunoassay (EIA). Y22 also reacts with fibrin of rabbits, rats, sheep, and dogs. The antibodies are of the IgG1 kappa-type and appear to be directed against a conformation-dependent epitope in the D-domain of fibrin. Experiments with 99mTc-labeled Y22 in vitro show that Y22 binds rapidly to forming clots. 99mTc-Y22 also binds to preformed plasma clots in a plasma milieu, even in the presence of high concentrations of heparin. Clot localization experiments in rabbits and rats confirm the high fibrin specificity and the potential of 99mTc-Y22 for thrombus imaging in vivo.
Wasser
M N
MN
Gaubius Institute TNO, Leiden, The Netherlands.
Koppert
P W
PW
Arndt
J W
JW
Emeis
J J
JJ
Feitsma
R I
RI
Pauwels
E K
EK
Nieuwenhuizen
W
W
D000005:Abdomen / Q000098:blood supply; D000818:Animals; D000911:Antibodies, Monoclonal; D001666:Binding Sites, Antibody; D005337:Fibrin / Q000276:immunology; D005340:Fibrinogen / Q000276:immunology; D007124:Immunoenzyme Techniques; D007601:Jugular Veins; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D011877:Radionuclide Imaging; D051381:Rats; D013667:Technetium; D013927:Thrombosis / Q000000981:diagnostic imaging*; D014682:Vena Cava, Inferior
D000911:Antibodies, Monoclonal; D013667:Technetium; D005337:Fibrin; D005340:Fibrinogen