title: An antifibrin monoclonal antibody useful in immunoscintigraphic detection of thrombi.
abstract: Balb/c mice were immunized with human plasmin-generated fibrinogen degradation product Y. Spleen cells were fused with P3X63-Ag8.653 myeloma cells. A clone (Y22) was found that produces monoclonal antibodies (MoAbs) with a strong reactivity with human fibrin and only a weak reactivity with fibrinogen in an enzyme immunoassay (EIA). Y22 also reacts with fibrin of rabbits, rats, sheep, and dogs. The antibodies are of the IgG1 kappa-type and appear to be directed against a conformation-dependent epitope in the D-domain of fibrin. Experiments with 99mTc-labeled Y22 in vitro show that Y22 binds rapidly to forming clots. 99mTc-Y22 also binds to preformed plasma clots in a plasma milieu, even in the presence of high concentrations of heparin. Clot localization experiments in rabbits and rats confirm the high fibrin specificity and the potential of 99mTc-Y22 for thrombus imaging in vivo.
authors.affiliation: Gaubius Institute TNO, Leiden, The Netherlands.
authors.forename: M N
authors.initials: MN
authors.lastname: Wasser
authors.forename: P W
authors.initials: PW
authors.lastname: Koppert
authors.forename: J W
authors.initials: JW
authors.lastname: Arndt
authors.forename: J J
authors.initials: JJ
authors.lastname: Emeis
authors.forename: R I
authors.initials: RI
authors.lastname: Feitsma
authors.forename: E K
authors.initials: EK
authors.lastname: Pauwels
authors.forename: W
authors.initials: W
authors.lastname: Nieuwenhuizen
mesh_terms: D000005:Abdomen / Q000098:blood supply; D000818:Animals; D000911:Antibodies, Monoclonal; D001666:Binding Sites, Antibody; D005337:Fibrin / Q000276:immunology; D005340:Fibrinogen / Q000276:immunology; D007124:Immunoenzyme Techniques; D007601:Jugular Veins; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D011877:Radionuclide Imaging; D051381:Rats; D013667:Technetium; D013927:Thrombosis / Q000000981:diagnostic imaging*; D014682:Vena Cava, Inferior
chemical_list: D000911:Antibodies, Monoclonal; D013667:Technetium; D005337:Fibrin; D005340:Fibrinogen