Reactivities of immunoglobulin E and immunoglobulin G subclasses identified by isoelectric focusing-immunoprint in allergic patients.
Probing of IgE or IgG subclass reactivities on single allergenic components in an extract is tedious and time-consuming under native conditions. Isoelectric focusing immunoblotting combines a powerful, highly resolving native separation method with the specificity and sensitivity of immunological test methods. This method is easy and quick to perform. The potential usefulness of this method is demonstrated by eight examples of a type I or type III allergy analyzing IgE and IgG subclass reactivities and their distribution.
Becker
W M
WM
Forschunginstitut Borstel, Federal Republic of Germany.
D000485:Allergens / Q000276:immunology*; D001232:Aspergillus fumigatus / Q000276:immunology; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D007073:Immunoglobulin E / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D007525:Isoelectric Focusing / Q000379:methods*; D006109:Poaceae / Q000276:immunology; D011058:Pollen / Q000276:immunology; D012130:Respiratory Hypersensitivity / Q000175:diagnosis / Q000276:immunology*
D000485:Allergens; D007074:Immunoglobulin G; D007073:Immunoglobulin E