Transrepression of the N-myc expression by c-myc protein.
In the human neuroblastoma cell line IMR32 the N-myc gene happens to be amplified and actively expressed, whereas no stable c-myc RNA can be detected in the same cells. In this report, we show that in IMR32 cells the expression of the N-myc gene is repressed by introduction of a c-myc expression vector (c-myc cDNA conjugated with an SR promoter). Moreover, dose response experiments showed that the amount of endogenous c-myc protein present in HeLa cells (which express c-myc but not N-myc) is enough to repress the expression of N-myc in IMR32 cells.
Kubota
Y
Y
Institute of Medical Science, University of Tokyo, Japan.
Kim
S H
SH
Iguchi-Ariga
S M
SM
Ariga
H
H
D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation; D006801:Humans; D009447:Neuroblastoma; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc; D012097:Repressor Proteins / Q000502:physiology*; D013997:Time Factors; D014157:Transcription Factors / Q000502:physiology*; D014162:Transfection; D014407:Tumor Cells, Cultured
D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D012097:Repressor Proteins; D014157:Transcription Factors