Enzyme catalyzed acylation and deacylation of the sugar moieties of nucleosides.
We have found that a lipase from Pseudomonas fluorescens (PFL) accelerated regioselective acylation of 2'-deoxynucleosides with the use of acid anhydrides as acyl donor in dry polar solvents. Different regioselective deacylation of 3',5'-di-O-acyl-2'-deoxynucleosides was found to take place when a lipase (PFL) or a protease from Bacillus subtilis (Subtilisin) was used.
Nozaki
K
K
Chemical Synthesis Research Laboratory, TAIHO Pharmaceutical Co., Ltd., Saitama, Japan.
Uemura
A
A
Yamashita
J
J
Yasumoto
M
M
D000215:Acylation; D001711:Biotransformation; D008049:Lipase / Q000378:metabolism; D009705:Nucleosides / Q000378:metabolism*; D013381:Subtilisins / Q000378:metabolism
D009705:Nucleosides; D008049:Lipase; D013381:Subtilisins