Human chromosome 12 is required for elevated HIV-1 expression in human-hamster hybrid cells.
Host cell factors act together with regulatory genes of the human immunodeficiency virus (HIV) to control virus production. Human-Chinese hamster ovary hybrid cell clones were used to probe for human chromosomes involved in regulating HIV gene expression. DNA transfection experiments showed that 4 of 18 clones had high levels of HIV gene expression measured by both extracellular virus production and transactivation of the HIV long terminal repeat in the presence of the trans-activator (tat) gene. Karyotype analyses revealed a 94% concordance (17/18) between human chromosome 12 and HIV gene expression. Other chromosomes had an 11 to 72% concordance with virus production.
Hart
C E
CE
Centers for Disease Control, Atlanta, GA 30333.
Ou
C Y
CY
Galphin
J C
JC
Moore
J
J
Bacheler
L T
LT
Wasmuth
J J
JJ
Petteway
S R
SR
Schochetman
G
G
D000818:Animals; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D002881:Chromosomes, Human, Pair 12; D006224:Cricetinae; D003412:Cricetulus; D015967:Gene Expression Regulation, Viral / Q000235:genetics*; D015756:Genes, tat; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D012091:Repetitive Sequences, Nucleic Acid; D015533:Transcriptional Activation
D015500:Chloramphenicol O-Acetyltransferase