Quantitative characterization of yeast cell incorporation in phagocytes: microscopic methods for differentiation between adherent and ingested particles.
A light- and a fluorescence-microscopic method for quantitative assessment of yeast cell incorporation in phagocytes were developed. The light-microscopic method offers methylene blue prestained yeast cells as phagocytosis particles and counterstains nonincorporated yeasts with eosine. The fluorescence-microscopic method works by acridine orange staining of phagocytosis assays. Fluorescence of nonincorporated yeast cells is suppressed by addition of methylene blue. Different ways of evaluating the results of microscopic quantitation of phagocytosis are discussed.
Stahl
M
M
Reifenberg
K
K
Lösch
U
U
D000165:Acridine Orange; D000818:Animals; D002417:Cattle; D002448:Cell Adhesion; D008751:Methylene Blue; D008856:Microscopy, Fluorescence; D010586:Phagocytes / Q000276:immunology*; D010587:Phagocytosis; D012441:Saccharomyces cerevisiae / Q000276:immunology*
D000165:Acridine Orange; D008751:Methylene Blue