Double-strand breaks stimulate alternative mechanisms of recombination repair.
To test the double-strand break repair model, we used HO nuclease to introduce double-strand breaks at several sites along a yeast chromosome containing duplicated DNA. Depending on the configuration of the double-strand break and recombining markers, different spectra of recombinant products were observed. Different repair kinetics and recombinant products were observed when a double-strand break was introduced in unique or duplicated DNA. The results of this study suggest that double-strand breaks in yeast stimulate recombination by several mechanisms, and we propose an alternative mechanism for double-strand break-induced gene conversion that does not depend on direct participation of the broken ends.
Nickoloff
J A
JA
Department of Molecular Biology, Scripps Clinic and Research Foundation, La Jolla, CA 92037.
Singer
J D
JD
Hoekstra
M F
MF
Heffron
F
F
D000483:Alleles; D004247:DNA / Q000235:genetics*; D004260:DNA Repair; D004271:DNA, Fungal / Q000235:genetics*; D005785:Gene Conversion; D005800:Genes, Fungal; D007700:Kinetics; D008957:Models, Genetic; D005810:Multigene Family; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae
D004271:DNA, Fungal; D004247:DNA