title: Microcolumn separations and the analysis of single cells.
abstract: Capillary zone electrophoresis and open tubular liquid chromatography are two examples of an emerging area of analytical instrumentation known as microcolumn separations. The high resolution and small sample requirements of these methods make them suitable for the quantitative, multicomponent chemical analysis of single cells. Appropriate instrumentation for the analysis of nanoliter and subnanoliter samples is discussed. Data from the analysis of individual neurons are presented, including amino acid and neurotransmitter content.
authors.affiliation: Department of Chemistry, University of North Carolina, Chapel Hill 27599.
authors.forename: R T
authors.initials: RT
authors.lastname: Kennedy
authors.forename: M D
authors.initials: MD
authors.lastname: Oates
authors.forename: B R
authors.initials: BR
authors.lastname: Cooper
authors.forename: B
authors.initials: B
authors.lastname: Nickerson
authors.forename: J W
authors.initials: JW
authors.lastname: Jorgenson
mesh_terms: D000596:Amino Acids / Q000032:analysis; D002477:Cells / Q000032:analysis*; D002853:Chromatography, Liquid / Q000295:instrumentation*; D004586:Electrophoresis / Q000295:instrumentation*; D005544:Forecasting; D008832:Microchemistry / Q000295:instrumentation
chemical_list: D000596:Amino Acids