title: Yeast 2 micron vectors replicate and undergo recombination in Torulaspora delbrueckii.
abstract: In order to develop a procedure for transformation of the industrial yeast Torulaspora delbrueckii, we have constructed a set of recombinant plasmids carrying Saccharomyces cerevisiae ARS and 2 microns origin of replication and kanamycin-G418 resistance gene of Tn903(601) as a selective marker. In this paper we show that S. cerevisiae ARS vectors can replicate autonomously and that vectors bearing the whole S. cerevisiae 2 microns sequence yield stable transformants. We also present evidence to show that 2 microns vectors undergo an FLP-mediated inter- and intramolecular recombination, which suggests that T. delbrueckii can support the amplification and partition mechanisms of these plasmids.
authors.affiliation: Dipartimento di Fisiologia e Biochimica Generali, Universita' di Milano, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Compagno
authors.forename: B M
authors.initials: BM
authors.lastname: Ranzi
authors.forename: E
authors.initials: E
authors.lastname: Martegani
mesh_terms: D015139:Blotting, Southern; D015870:Gene Expression; D005822:Genetic Vectors; D007763:Lac Operon; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D015183:Restriction Mapping; D012440:Saccharomyces / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014170:Transformation, Genetic