Estimating the degree of infection of Plasmodium berghei infected red blood cells by evaluation of pyruvate kinase activity.
A procedure is proposed to estimate the parasitemia of red blood cells infected with P. berghei by determination of pyruvate kinase activity. The activity of this enzyme shows a linear dependence not on the relative number of infected cells but on the average number of parasites per red blood cell. To provide a transformation between these two parameters a theoretical model of multiple infections of blood cells is derived, the accuracy of which was proved experimentally.
Buckwitz
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Institute of Biochemistry, School of Medicine (Charité), Humboldt-University, Berlin, G.D.R.
Jacobasch
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Break
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D000818:Animals; D004912:Erythrocytes / Q000201:enzymology* / Q000469:parasitology; D008288:Malaria / Q000201:enzymology* / Q000469:parasitology; D051379:Mice; D010962:Plasmodium berghei / Q000302:isolation & purification; D011336:Probability; D011770:Pyruvate Kinase / Q000378:metabolism*; D051381:Rats; D012044:Regression Analysis
D011770:Pyruvate Kinase