Cloning and expression in Escherichia coli of the Azospirillum brasilense Sp7 gene encoding ampicillin resistance.
The Azospirillum brasilense ATCC 29145 gene coding for beta-lactamase was cloned in Escherichia coli. The gene was expressed in E. coli from its own promoter as a 30-kilodalton protein, conferring resistance to high levels of beta-lactam antibiotics. The DNA sequence containing the beta-lactamase gene was found to be highly amplified in the Azospirillum genome, scattered in the chromosomal as well as in the plasmidic DNA.
Verreth
C
C
F. A. Janssens Memorial Laboratory for Genetics, University of Leuven, Heverlee, Belgium.
Cammue
B
B
Swinnen
P
P
Crombez
D
D
Michielsen
A
A
Michiels
K
K
Van Gool
A
A
Vanderleyden
J
J
D000668:Ampicillin Resistance / Q000235:genetics*; D015139:Blotting, Southern; D002499:Centrifugation, Density Gradient; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics*
D004269:DNA, Bacterial; D001618:beta-Lactamases