Myosin heavy-chain isoforms in adult and developing rabbit vascular smooth muscle.
Two monoclonal antibodies specific for smooth muscle myosin (designated SM-E7 and SM-A9) and one monoclonal anti-(human platelet myosin) antibody (designated NM-G2) have been used to study myosin heavy chain composition of smooth muscle cells in adult and in developing rabbit aorta. Sodium dodecyl sulfate/polyacrylamide gel electrophoresis and Western blotting experiments revealed that adult aortic muscle consisted of two myosin heavy chains (MCH) of smooth muscle type, named MHC-1 (205 kDa), and MHC-2 (200 kDa). In the fetal/neonatal stage of development, vascular smooth muscle was found to contain only MHC-1 but not MHC-2. Non-muscle myosin heavy chain, which showed the same electrophoretic mobility as the slower migrating MHC, was expressed in an inverse manner with respect to MHC-2, i.e. it was detectable only in the early stages of development. The distinct pattern of smooth and non-muscle myosin isoform expression during development may be related to the different functional properties of smooth muscle cells during vascular myogenesis.
Borrione
A C
AC
Institute of General Pathology, University of Padova, Italy.
Zanellato
A M
AM
Scannapieco
G
G
Pauletto
P
P
Sartore
S
S
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D001013:Aorta, Thoracic; D015153:Blotting, Western; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D024510:Muscle Development; D009131:Muscle, Smooth, Vascular / Q000196:embryology / Q000254:growth & development* / Q000378:metabolism; D009218:Myosins / Q000276:immunology / Q000378:metabolism*; D011817:Rabbits
D000911:Antibodies, Monoclonal; D009218:Myosins