[Synthesis and expression of an artificial gene of an IgG-binding fragment of protein A from Staphylococcus aureus].
The chemical-enzymatic synthesis of a gene for IgG-binding fragment of the staphylococcal protein A has been carried out. The design of the gene, which consists of signal peptide and modified E and B domains, and strategy of the synthesis provided possibility of various degrees of polymerization of the gene fragment coding for B domain and of the whole gene. Several protein A-like polypeptides composed of the leader sequence, E domain and 1 to 4 copies of B domain were produced in E. coli cells under the lac promoter control.
Efimov
V A
VA
Buriakova
A A
AA
Polushin
N N
NN
Pashkova
I N
IN
Dmitrakova
E V
EV
D000595:Amino Acid Sequence; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005813:Genes, Synthetic; D007128:Immunoglobulin Fragments / Q000235:genetics*; D008969:Molecular Sequence Data; D013205:Staphylococcal Protein A / Q000235:genetics*; D013211:Staphylococcus aureus / Q000235:genetics* / Q000276:immunology
D007128:Immunoglobulin Fragments; D013205:Staphylococcal Protein A
Sintez i ékspressiia iskusstvennogo gena IgG-sviazyvaiushchego fragmenta bela A Staphylococcus aureus.