title: [Complementary addressed alkylation of 16s rRNA from Escherichia coli by 2',3'-0-[4-N-(2-chloroethyl)-N-methylamino]benzylidene derivatives of oligodeoxyribonucleotides. III. Segments of 16s rRNA interacting with the benzylidene derivative of d(pACCTTGTT)rA].
abstract: 16S rRNA chain was cleaved by RNase H within covalent adduct with a 2',3'-0-[4-N-(2-chloroethyl)-N-methylamino]benzylidene derivative of d(pACCTTGTT)rA. It was shown that no less than 50% of cleavage occurs at the 1498-1506 site. The selectivity of alkylation and correspondingly the cleavage by RNase H, at this site practically does not increase when RNA was alkylated to a low extent, and also when a small excess of the reagent in respect to rRNA was used.
authors.forename: M A
authors.initials: MA
authors.lastname: Zenkova
authors.forename: G G
authors.initials: GG
authors.lastname: Karpova
authors.forename: A S
authors.initials: AS
authors.lastname: Levina
mesh_terms: D000478:Alkylation; D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009588:Nitrogen Mustard Compounds / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000378:metabolism*
chemical_list: D009588:Nitrogen Mustard Compounds; D009838:Oligodeoxyribonucleotides; D012335:RNA, Ribosomal; D012336:RNA, Ribosomal, 16S
vernacular_title: Komplementarno adresovannoe alkilirovanie 16S rRNK Escherichia coli 2',3'-0-[4-N-(2-khlorétil)-N-metilamino]benzilidenovymi proizvodnymi oligodezoksiribonukleotidov. III. uchastki 16S rRNA, vzaimodeĭstvuiushchie s benzilidenovym proizvodnym d(pACCTTGTT)rA.