[The effect of the oligomerization of pBR322 on the level of transformation in Escherichia coli].
The ability of the known Escherichia coli strain JC3881 recB recC recF sbc15 to produce oligomeric and multimeric forms of pBR322 underlies the study presented. The individual oligomeric forms of pBR322 were isolated from the agarose gel. The plasmid forms were used for electron microscopic control and also introduced into the system of E. coli competent cells. The E. coli transformation level of different forms of plasmid DNA rose from monomers to pentamers. CCC forms of the plasmid possessed high efficiency of the E. coli cell transformation. The systems of the host recombination are to be significant in the process of plasmid oligomerization.
Fomin
V V
VV
Telegeev
G D
GD
Maliuta
S S
SS
D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics / Q000648:ultrastructure; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000032:analysis / Q000235:genetics* / Q000648:ultrastructure; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D010957:Plasmids / Q000235:genetics*; D014169:Transformation, Bacterial / Q000235:genetics*
D004269:DNA, Bacterial; D046911:Macromolecular Substances
Vliianie oligomerizatsii pBR322 na uroven' transformatsii Escherichia coli.