Characterization of monoclonal antibodies against human parvovirus B19.
Eleven hybridoma cell lines producing mouse monoclonal antibodies (mAbs) against human parvovirus B19 were established. Their specificity was as follows. Approximately 5% of fetal erythroid cells inoculated with B19 reacted with all the mAbs and with anti-B19 positive human serum, but not with negative serum by indirect double immunofluorescence staining. All the mAbs recognized both VP-1 (84 kDa) and VP-2 (58 kDa) capsid proteins of B19 virions propagated in vitro and in vivo by Western blotting, and immunoprecipitated B19 virions.
Yaegashi
N
N
Department of Microbiology, Tohoku University School of Medicine, Miyagi.
Tada
K
K
Shiraishi
H
H
Ishii
T
T
Nagata
K
K
Sugamura
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000276:immunology; D000918:Antibody Specificity; D015139:Blotting, Southern; D015153:Blotting, Western; D004279:DNA, Viral / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006825:Hybridomas; D051379:Mice; D010321:Parvoviridae / Q000276:immunology*; D011233:Precipitin Tests; D014771:Virion / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D004279:DNA, Viral