Titration of Coxiella burnetii in Buffalo green monkey (BGM) cell cultures.
Titration of Coxiella burnetii, strain Nine Mile, phase I, and strain Frankfurt, phase II, in Buffalo green monkey (BGM) cover slip cell cultures yielded reproducible infectivity titers after centrifugation of infected cell cultures (3000 g, 30 min, 37 degrees C) and a subsequent 4-day incubation at 37 degrees C in Minimal Essential Medium (MEM). Compared to other titration procedures (plaque assays, titration in embryonated chicken eggs), this technique proved to be fast, less laborious and economical.
Schneider
W
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Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität, Giessen.
D000818:Animals; D001431:Bacteriological Techniques; D002478:Cells, Cultured; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D003381:Coxiella / Q000187:drug effects / Q000254:growth & development / Q000472:pathogenicity*; D003513:Cycloheximide / Q000494:pharmacology; D051379:Mice
D003513:Cycloheximide