[Use of an immunoperoxidase method in situ for detecting recombinant clones of vaccinia virus containing an expressed gene of influenza viral hemagglutinins].
The vaccine L-IVP strain of vaccinia virus (VV) was used to construct the recombinant viral clones containing the influenza A hemagglutinin gene. The recombinant T plasmid was obtained with HA gene inserted in the vector pGS-20 (B. Moss) under the 7.5 K promoter of VV. A homologous recombination technique was used to insert the gene with the flanking TK sequences into vaccinia virus genome. The recombinant clones were selected by dot-hybridization with [32P]-labeled HA-probe. These recombinants were analysed for HA gene expression by the indirect immunoperoxidase method in situ using the peroxidase conjugate of the staphylococcal A-protein. This technique allows to obtain stable stained preparation and analyse the protein behavior at the ultrastructural level.
Selivanova
T K
TK
Leonov
S V
SV
Filatov
F P
FP
Iuferov
V P
VP
Garaev
M M
MM
Bobkov
A F
AF
Rassulin
Iu Iu
IuIu
Khizhniakova
T M
TM
Uryvaev
L V
LV
Kharitonenkov
I G
IG
D002999:Clone Cells; D004279:DNA, Viral / Q000235:genetics; D015870:Gene Expression; D005814:Genes, Viral; D006389:Hemagglutinins, Viral / Q000235:genetics*; D007124:Immunoenzyme Techniques; D009980:Influenza A virus / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D013205:Staphylococcal Protein A; D014616:Vaccinia virus / Q000235:genetics*
D004279:DNA, Viral; D006389:Hemagglutinins, Viral; D013205:Staphylococcal Protein A
Ispol'zovanie immunoperoksidaznogo metoda in situ dlia vyiavleniia rekombinantnykh klonov virusa ospovaktsiny, soderzhashchikh ékspressiruemyĭ gen gemaggliutinina virusa grippa.