Cloning of a beta-tubulin gene from Plasmodium falciparum.
We describe the isolation and characterization of a gene for beta-tubulin from the malaria parasite, Plasmodium falciparum. This organism appears to contain a single gene encoding beta-tubulin. A single transcript from this gene can be detected in the total RNA of the parasite's asexual blood stages. The complete sequence for the gene has been elucidated. It has two introns, one of which has a position identical to that of a related parasite, Toxoplasma gondii. The gene shows the usual preference for codons with A or T in the third position. The predicted amino acid sequence is compared with that of T. gondii and the human host. Further comparisons between these and fungal sequences of beta-tubulins resistant to benomyl, a drug binding this protein, highlight differences that could be exploited in the development of parasite-specific antitubulin drugs.
Delves
C J
CJ
Department of Molecular Biology, University of Edinburgh, UK.
Ridley
R G
RG
Goman
M
M
Holloway
S P
SP
Hyde
J E
JE
Scaife
J G
JG
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001562:Benzimidazoles / Q000494:pharmacology; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004351:Drug Resistance; D005658:Fungi / Q000235:genetics; D015870:Gene Expression; D005796:Genes; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D014122:Toxoplasma / Q000235:genetics; D014404:Tubulin / Q000235:genetics*
D001562:Benzimidazoles; D014404:Tubulin; D004247:DNA