Antisperm antibody tests: traditional methods compared to ELISA.
Enzyme-linked immunosorbent assays (ELISAs) to detect sperm antibodies can be a useful addition to other tests. ELISAs allow more quantitative and detailed information than some other tests, but results should be compared to those of other tests. This study was conducted to correlate results of an ELISA that used a sperm membrane extract as antigen with those of other tests. Semen, seminal plasma, and serum of 34 sperm-antibody-positive and 36 sperm-antibody-negative men were tested. There was poor correlation between the ELISA results and those of the other tests. Only the results of a direct immunobead test done on semen correlated fairly well with results from ELISAs done on serum. Further studies are needed on fertility-specific antigens that can be identified and isolated and then studied by means of ELISAs.
Windt
M L
ML
Department of Obstetrics and Gynaecology, Tygerberg Hospital, Republic of South Africa.
Bouic
P J
PJ
Lombard
C J
CJ
Menkveld
R
R
Kruger
T F
TF
D000906:Antibodies / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007158:Immunologic Techniques; D007248:Infertility, Male / Q000097:blood / Q000276:immunology*; D008297:Male; D012661:Semen / Q000276:immunology; D013094:Spermatozoa / Q000276:immunology*
D000906:Antibodies