[Substrate specificity of the biotransformation enzymes in a Nocardia erythropolis culture].
Substrate specificity of biotransformation enzymes of culture Nocardia erythropolis was studied. Products of transformation of cholesterol and three sterols of microbial origin: ergosterol, ergosta-5,7-dien-3 beta-ol and ergosta-7,22-dien-3 beta-ol was identified with a help of thin-layer chromatography, UV spectrophotometry and mass-spectrometry. It was established, that delta 22-bond in the side chains of sterols and delta 7-bond slows and delta 5-bond makes impossible cleavage of side chains of sterols.
Klimashina
M M
MM
Viktorovskiĭ
I V
IV
Mikhaĭlova
N P
NP
V'iunov
K A
KA
D001711:Biotransformation; D002784:Cholesterol / Q000032:analysis / Q000493:pharmacokinetics; D002855:Chromatography, Thin Layer; D004875:Ergosterol / Q000031:analogs & derivatives / Q000032:analysis / Q000493:pharmacokinetics; D013058:Mass Spectrometry; D009154:Mutation; D009615:Nocardia / Q000201:enzymology*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
C012287:22,23-dihydroergosterol; C059438:ergosta-7,22-dien-3-ol; D002784:Cholesterol; D004875:Ergosterol
Izuchenie substratnoĭ spetsifichnosti fermentov biotransformatsii kul'tury Nocardia erthropolis.