DNA replication origin and transcriptional enhancer in c-myc gene share the c-myc protein binding sequences.
We have previously reported that c-myc protein, or protein(s) complexed with c-myc protein, binds to the region upstream of the first exon of the c-myc gene and that this region contains an origin of cellular DNA replication (ori) and also a transcriptional enhancer. Here we show by Southwestern blotting that c-myc protein binds directly to a 7 bp sequence within the above region. Furthermore, we show that the c-myc protein binding sequences are indispensable for both ori and enhancer functions, but that additional sequences are required for maximal ori and enhancer activities. Thus, c-myc protein is a sequence specific factor which is apparently used both in initiation of DNA replication and in regulation of RNA transcription.
Ariga
H
H
Faculty of Pharmaceutical Science, Hokkaido University, Sapporo, Japan.
Imamura
Y
Y
Iguchi-Ariga
S M
SM
D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D004261:DNA Replication; D003850:Deoxyribonuclease I; D004742:Enhancer Elements, Genetic; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D007739:L Cells / Q000201:enzymology; D051379:Mice; D008969:Molecular Sequence Data; D009710:Nucleotide Mapping; D010957:Plasmids; D011485:Protein Binding; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc; D011519:Proto-Oncogenes; D014158:Transcription, Genetic
D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D015500:Chloramphenicol O-Acetyltransferase; D011505:Protein-Tyrosine Kinases; D003850:Deoxyribonuclease I