A simple procedure for the isolation of neurons from the CA1 and CA3 region of the postnatal rat hippocampus.
A procedure was developed for the isolation of pyramidal neurons from defined regions (CA1, CA3) of the postnatal rat hippocampus. By a quantitative approach the influence of various parameters (pO2,glucose, enzymes, temperature, buffers, [Ca++]0, mechanical isolation) was evaluated on number and state of viability of the isolated cells using the nigrosine exclusion test and considering the neurons' morphological features. Cell viability of disaggregated Ca1- and CA3-neurons was compared. These isolated neurons are well suited for whole-cell patch-clamp measurements as well as for pharmacological investigations.
Steinhäuser
C
C
Academy of Sciences of the GDR, Institute of Neurobiology and Brain Research, Magdeburg.
Gündel
J
J
Matthies
H
H
D000818:Animals; D002021:Buffers; D002118:Calcium / Q000494:pharmacology; D003584:Cytological Techniques; D005260:Female; D005947:Glucose / Q000494:pharmacology; D006624:Hippocampus / Q000033:anatomy & histology / Q000166:cytology*; D009474:Neurons / Q000166:cytology*; D010100:Oxygen / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013696:Temperature; D014357:Trypsin / Q000494:pharmacology
D002021:Buffers; D014357:Trypsin; D005947:Glucose; D010100:Oxygen; D002118:Calcium