Method for improved extraction of DNA from Nocardia asteroides.
In a variation of standard DNA extraction methods, Nocardia asteroides was repeatedly exposed to sodium dodecyl sulfate at 60 degrees C for 30 min; each extraction was followed by centrifugation, removal of the nucleic acid-rich supernatant, and suspension of the cell pellet in fresh sodium dodecyl sulfate. The pooled supernatants contained a substantially higher amount of DNA than the first supernatant alone. The possible implications of this procedure on the development of DNA probes are discussed.
Loeffelholz
M J
MJ
Diagnostic Hybrids, Inc., Athens, Ohio 45701.
Scholl
D R
DR
D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D009616:Nocardia asteroides / Q000235:genetics*; D012967:Sodium Dodecyl Sulfate
D015342:DNA Probes; D004269:DNA, Bacterial; D012967:Sodium Dodecyl Sulfate