Infection and uptake of duck hepatitis B virus by duck hepatocytes maintained in the presence of dimethyl sulfoxide.
Primary duck hepatocytes maintained in serum-free culture medium containing dimethyl sulfoxide support efficient replication of duck hepatitis B virus following infection in vitro. Cells remain susceptible to infection for at least 2 weeks after plating, allowing spread of virus via repeat cycles of infection. Up to 100% of cultured hepatocytes can be infected by prolonged exposure to high titer virus inoculum at 37 degrees. We have identified a fraction of infecting virus which binds tightly to cells at 4 degrees, presumably due to association with high affinity receptors on the hepatocyte membrane. The rate at which tightly bound virus is internalized at 37 degrees appears slow, with uptake occurring over a period of at least 16 hr.
Pugh
J C
JC
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
Summers
J W
JW
D000327:Adsorption; D000818:Animals; D015139:Blotting, Southern; D002478:Cells, Cultured; D003470:Culture Media; D004261:DNA Replication; D004279:DNA, Viral / Q000032:analysis*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004372:Ducks; D005455:Fluorescent Antibody Technique; D015611:Hepatitis B Virus, Duck / Q000378:metabolism / Q000502:physiology*; D008099:Liver / Q000166:cytology / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D013696:Temperature; D014779:Virus Replication
D003470:Culture Media; D004279:DNA, Viral; D004121:Dimethyl Sulfoxide