Efficient retroviral transfer of a mouse c-myc construct into HL60.
We introduced an LTR-driven mouse c-myc second and third exon, Tn5Neo gene construct into the inducible human leukemia line HL60 using an amphotropic retroviral vector system. Over 90% of the cells became neo-resistant and the transfected myc gene was transcribed in several neomycin resistant clones. Making use of the simultaneous presence of the different myc genes in the same cell, we compared expression of the corresponding mRNAs after differentiation and their decay mechanisms.
Szabó
G
G
Department of Tumor Biology, Karolinska Institutet, Stockholm, Sweden.
Winberg
G
G
Henriksson
M
M
Klein
G
G
Sümegi
J
J
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger / Q000235:genetics; D012190:Retroviridae / Q000235:genetics; D014162:Transfection
D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger