[Loading of polystyrene solid phases with microbial agents and their polysaccharide antigens for the screening of monoclonal antibodies].
Before screening monoclonal antibodies using enzyme immuno assays, it is necessary to prove that the coating of the solid phases occurs efficiently. The adhesion of whole cells of group B streptococci, Escherichia coli K1, Haemophilus influenzae type b and Candida albicans as well as B-Streptococcus group antigen, H. influenzae type b capsular antigen, and C. albicans cell wall antigen to polystyrene solid phases was studied and detected by the following methods: Peroxidase competition procedure, scintillation measurement of 32phosphate-labelled germs, and reactions with monoclonal antibodies.
Mehl
M
M
Institut für Medizinische Mikrobiologie, Humboldt-Universität zu Berlin.
Starke
R
R
Urban
A
A
Hoffmann
A
A
Kiessig
S
S
Schmidt
G
G
D000911:Antibodies, Monoclonal / Q000032:analysis*; D000942:Antigens, Bacterial / Q000378:metabolism; D000946:Antigens, Fungal / Q000378:metabolism; D001419:Bacteria / Q000378:metabolism*; D001422:Bacterial Adhesion; D001667:Binding, Competitive; D002176:Candida albicans / Q000378:metabolism*; D002448:Cell Adhesion; D004926:Escherichia coli / Q000378:metabolism; D006193:Haemophilus influenzae / Q000378:metabolism; D007124:Immunoenzyme Techniques; D011134:Polysaccharides / Q000378:metabolism*; D011137:Polystyrenes / Q000378:metabolism*; D012588:Scintillation Counting; D013292:Streptococcus agalactiae / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000946:Antigens, Fungal; D011134:Polysaccharides; D011137:Polystyrenes
Beladung von Polystyrol Festphasen mit mikrobiellen Erregern und deren Polysaccharid-Antigenen zum Screening monoklonaler Antikörper.