Single crystals of bacteriophage T7 RNA polymerase.
Single crystals of T7 RNA polymerase have been grown to a maximum size of 1.8 x 0.3 x 0.3 mm. The crystals are composed of fully intact T7 RNA polymerase which is enzymatically active upon dissolution. These crystals belong to the monoclinic space group P2(1) and have unit cell parameters a = 114.5 A, b = 139.6 A, c = 125.7 A, and beta = 98.1 degrees. Self-rotation function studies indicate that there are three molecules per asymmetric unit. The crystals diffract to at least 3.0 A resolution. These are the first crystals of a DNA-dependent RNA polymerase suitable for high-resolution X-ray structure determination.
Sousa
R
R
Department of Crystallography, University of Pittsburgh, Pennsylvania 15260.
Rose
J P
JP
Chung
Y J
YJ
Lafer
E M
EM
Wang
B C
BC
D003460:Crystallization; D012321:DNA-Directed RNA Polymerases / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology*; D008970:Molecular Weight; D011487:Protein Conformation; D013604:T-Phages / Q000201:enzymology*; D014961:X-Ray Diffraction
D012321:DNA-Directed RNA Polymerases