Radioimmunoassay of inhibin in the serum of male monkeys.
A heterologous inhibin radioimmunoassay method to measure inhibin in serum of male cynomolgus (Macaca fascicularis) and rhesus (Macaca mulatta) monkeys has been validated using a specific antibody against bovine 31 kDa inhibin and 125I-labelled 31 kDa inhibin as tracer. A serum pool from male monkeys was used as standard. Serial dilutions of normal monkey serum showed parallel logit-log dose-response curves to purified porcine and bovine inhibin as well as to a female human serum pool. The intra-assay coefficient of variation was 4.2% (n = 10) and the interassay coefficient of variation 5.1% (n = 10). No loss of inhibin immunoactivity was noted after storage at 23 degrees C for 5 days or repeated thawing and freezing of the serum samples. Serum from castrated monkeys showed undetectable levels of inhibin. Treatment with a gonadotrophin-releasing hormone agonist for 15 weeks led to a marked suppression of peripheral serum inhibin to concentrations similar to those after hypophysectomy or pituitary stalk section.
Fingscheidt
U
U
Max Planck Clinical Research Unit for Reproductive Medicine, University of Münster, F.R.G.
Weinbauer
G F
GF
Robertson
D M
DM
de Kretser
D M
DM
Nieschlag
E
E
D000818:Animals; D000918:Antibody Specificity; D004306:Dose-Response Relationship, Immunologic; D007016:Hypophysectomy; D007265:Inhibins / Q000097:blood*; D008252:Macaca fascicularis; D008253:Macaca mulatta; D008297:Male; D009919:Orchiectomy; D010906:Pituitary Hormone-Releasing Hormones / Q000037:antagonists & inhibitors; D011863:Radioimmunoassay / Q000379:methods*; D013997:Time Factors
D010906:Pituitary Hormone-Releasing Hormones; D007265:Inhibins