Purification of acidic fibroblast growth factor from bovine heart and its localization in the cardiac myocytes.
We found an endogenous growth factor, referred to here as heart-derived growth factor (HDGF), that stimulates the proliferation of vascular endothelial cells. HDGF was purified from bovine myocardium using a procedure that involves denaturation of undesired proteins with methanol and chloroform. Soluble HDGF was purified essentially to homogeneity in a single step by heparin affinity chromatography. The purified HDGF was identified to be acidic fibroblast growth factor based on the following properties: molecular weight of 18,000, isoelectric point of 5.2, amino acid composition and sequence, its dissociation from a heparin affinity column at 0.9 M NaCl, potentiation of activity in the presence of heparin, and antigenicity. Our yield of HDGF was 500 micrograms/kg of tissue. Antiserum raised to HDGF localized HDGF in the cardiac myocytes in culture. These data indicate that a large amount of acidic fibroblast growth factor is present in the heart, and the cardiac myocytes are likely to be a major source of it.
Sasaki
H
H
Bio-Science Laboratory Inc., Yamagata, Japan.
Hoshi
H
H
Hong
Y M
YM
Suzuki
T
T
Kato
T
T
Sasaki
H
H
Saito
M
M
Youki
H
H
Karube
K
K
Konno
S
S
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002455:Cell Division; D002846:Chromatography, Affinity; D004357:Drug Synergism; D004730:Endothelium, Vascular / Q000166:cytology; D005346:Fibroblast Growth Factors / Q000302:isolation & purification* / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology; D007118:Immunoassay; D007124:Immunoenzyme Techniques; D007526:Isoelectric Point; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009206:Myocardium / Q000032:analysis*; D010446:Peptide Fragments
D000596:Amino Acids; D010446:Peptide Fragments; D005346:Fibroblast Growth Factors; D006493:Heparin