Immunocytochemical detection of p21ras expression in fresh human leukaemic cells and cell lines.
The expression of p21ras proteins was investigated by immunocytochemistry in permanent cell lines and in fresh human leukaemic cells. While high and low levels of p21ras could be detected in most of the cell lines, no significant p21ras immunoreactivity was noted in cells of ten human acute and chronic leukaemias. Thus, notwithstanding its possible role in the initial transformation process in human leukaemias, p21ras expression appears not to be an irrevocable requirement for the maintenance of the transformed state.
Neubauer
A
A
Abteilung Innere Medizin, Klinikum Rudolf Virchow der Freien Universität, Berlin.
Herbst
H
H
Rochlitz
C
C
Siegert
W
W
Schmidt
C A
CA
Huhn
D
D
D000911:Antibodies, Monoclonal; D002460:Cell Line; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D007938:Leukemia / Q000235:genetics*; D015689:Oncogene Protein p21(ras) / Q000032:analysis / Q000235:genetics*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D015689:Oncogene Protein p21(ras)