[A study of the intracellular fate of recombinant human interleukin 2 in Escherichia coli].
The expression of the human IL-2 recombinant gene in E. coli cells was studied. The processes which take place during thermo-induced expression and effect the state of the product were investigated. Experimental data on the membrane localisation of IL-2, the formation of aggregates (inclusion bodies) and polymers were obtained. It was determined that temperature significantly influence the kinetics of the processes of intracellular IL-2 production and IL-2 stability. It is supposed that the cell membrane state plays a determining role in these processes via temperature mediation. Thus, the formation of inclusion bodies described for a number of E. coli recombinant strains is probably stipulated not only by recombinant polypeptides properties, but also by cellular interactions.
Meriin
A B
AB
Ivaniushina
V A
VA
Dobkin
A I
AI
Ogarkov
V I
VI
D001345:Autoradiography; D002462:Cell Membrane / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D007376:Interleukin-2 / Q000235:genetics* / Q000378:metabolism; D007700:Kinetics; D010957:Plasmids; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013696:Temperature
D007376:Interleukin-2; D011994:Recombinant Proteins
Issledovanie vnutrikletochnoĭ sud'by rekombinantnogo chelovecheskogo interleĭkina 2 v Escherichia coli.