[Biosynthesis and secretion of bovine growth hormone in Escherichia coli under the control of the secretory vector containing a promoter and signal region of the alkaline phosphatase gene].
A recombinant plasmid carrying a bovine growth hormone gene fused with the regulatory and signal regions of the alkaline phosphatase gene of E. coli was constructed. The bovine growth hormone gene expression as well as protein partial processing and secretion into the periplasm have been shown to take place under phosphate starvation, i.e. conditions of alkaline phosphatase derepression.
Tsfasman
I M
IM
Nesmeianova
V G
VG
Gorbulev
V G
VG
Rubtsov
P M
PM
Skriabin
K G
KG
D000469:Alkaline Phosphatase / Q000235:genetics*; D000818:Animals; D002417:Cattle; D002458:Cell Fractionation; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D005809:Genes, Regulator; D005822:Genetic Vectors; D013006:Growth Hormone / Q000096:biosynthesis* / Q000378:metabolism; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism
D011994:Recombinant Proteins; D013006:Growth Hormone; D000469:Alkaline Phosphatase
Biosintez i sekretsiia gormona rosta byka u Escherichia coli pod kontrolem sekretornogo vektora soderzhashchego promotor i signal'nuiu posledovatel'nost' gena shchelochnoĭ fosfatazy.