title: Assembly of Escherichia coli fumarate reductase holoenzyme.
abstract: The production and assembly of the four fumarate reductase polypeptides into holoenzyme was studied in vivo in a T7-promoter-conditional expression system. No posttranslational modification of any of the subunits was detected, although the ratio of polypeptides produced varied with the temperature at which expression occurred. FrdC and FrdD, the membrane anchor polypeptides, assembled rapidly into the membrane and then were capped with FrdA and FrdB in separate events. Truncation of the C-terminal domain of FrdD by insertion of transposon Tn5 into the frdD cistron interfered with membrane insertion of the anchor polypeptides and assembly of the holoenzyme. Proteolytic degradation of truncated FrdD was implicated in the production of a soluble FrdABC trimer.
authors.affiliation: Department of Biochemistry, University of Alberta, Edmonton, Canada.
authors.forename: D J
authors.initials: DJ
authors.lastname: Latour
authors.forename: J H
authors.initials: JH
authors.lastname: Weiner
mesh_terms: D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology*; D005838:Genotype; D010088:Oxidoreductases / Q000096:biosynthesis*; D044925:Oxidoreductases Acting on CH-CH Group Donors; D010957:Plasmids; D011233:Precipitin Tests; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D013604:T-Phages / Q000235:genetics; D013696:Temperature; D014170:Transformation, Genetic
chemical_list: D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C020155:fumarate reductase (NADH)