title: Controlled study comparing treatment with monocomponent insulin and conventional insulin in patients with lipoatrophy.
abstract: Twenty-one patients with evident lipoatrophy treated with conventional (Conv.) insulin were either allocated to continuation of treatment with previously used insulin (Conv. group, n = 10) or were transferred to Lente MC (monocomponent) insulin with or without supplementary Actrapid MC insulin (MC group, n = 11). On entry and after 3, 6 and 12 months of follow-up, serum insulin-, pancreatic polypeptide- and proinsulin-binding IgGs were determined by radioimmunoelectrophoresis according to the method of Christiansen. Prior to determination of proinsulin-binding IgG, the insulin-binding IgG was removed by means of sepharose-bound insulin according to the method of Heding. In both groups a slight decrease in the titer of insulin-binding IgG was observed: in the Conv. group from 5.33 +/- 0.92 (SEM) to 4.66 +/- 1.17 mU/ml after 12 months, and in the MC group from 3.22 +/- 0.64 to 2.66 +/- 0.46 mU/ml, respectively. Due to the small number of patients with pancreatic polypeptide antibody titers above the detection limit no statistical evaluation was carried out. The level of serum proinsulin-binding IgG decreased in the MC group only (from 9.3 +/- 2.2 to 1.9 +/- 0.6 ng/ml after 12 months), and even showed a slight increase in the Conv. group (the respective titers were: 14.0 +/- 4.6 and 14.9 +/- 4.6 ng/ml). In the MC group 10 patients (91%) showed improvement and 7 (64%) complete regression of their lipoatrophy corresponding to 6 (60%) and 2 (20%) in the Conv. group. This finding suggests a possible role of proinsulin-binding antibodies in the pathogenesis of insulin lipoatrophy.
authors.affiliation: Klinika Gastroenterologii i Chrorób Przemiany Materii, Akademii Medycznej w Warszawie, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Czyzyk
authors.forename: H
authors.initials: H
authors.lastname: Rogala
authors.forename: J
authors.initials: J
authors.lastname: Lawecki
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects / Q000473:pathology*; D000328:Adult; D001284:Atrophy; D003920:Diabetes Mellitus / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007328:Insulin / Q000009:adverse effects* / Q000627:therapeutic use; D007330:Insulin Antibodies / Q000032:analysis; D049528:Insulin, Long-Acting / Q000627:therapeutic use*; D008297:Male; D010191:Pancreatic Polypeptide / Q000276:immunology; D011384:Proinsulin / Q000276:immunology
chemical_list: D007074:Immunoglobulin G; D007328:Insulin; D007330:Insulin Antibodies; D049528:Insulin, Long-Acting; D010191:Pancreatic Polypeptide; D011384:Proinsulin