title: LN-6: a monoclonal antibody to vimentin expressed in non-hematopoietic mesenchymal cells and derived tumors and reactive in B5-fixed, paraffin-embedded tissues.
abstract: We generated a monoclonal antibody (MAb), designated LN-6, directed against human vimentin, which retains its immunoreactivity in B5-fixed, paraffin-embedded tissues. Like other anti-vimentin MAb, LN-6 was found to be reactive with a wide spectrum of human sarcomas and normal cells of mesenchymal derivation. However, unlike other similar reagents, LN-6 was unreactive with normal and malignant human lymphoid cells and therefore displays a more restricted immunoreactivity. Because of its ability to stain routinely processed pathological tissues and its marked reactivity with human sarcomas, LN-6 is a unique reagent for the immunohistochemical diagnosis of human cancer.
authors.affiliation: Department of Pathology, University of Southern California School of Medicine, Los Angeles 90033.
authors.forename: E
authors.initials: E
authors.lastname: Stathopoulos
authors.forename: G S
authors.initials: GS
authors.lastname: Naeve
authors.forename: C R
authors.initials: CR
authors.lastname: Taylor
authors.forename: A L
authors.initials: AL
authors.lastname: Epstein
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002277:Carcinoma / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology; D005455:Fluorescent Antibody Technique; D005557:Formaldehyde; D006652:Histological Techniques; D006801:Humans; D007124:Immunoenzyme Techniques; D008223:Lymphoma / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology; D008648:Mesoderm / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D010232:Paraffin; D011233:Precipitin Tests; D012983:Soft Tissue Neoplasms / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology*; D014746:Vimentin / Q000276:immunology / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D014746:Vimentin; D005557:Formaldehyde; D010232:Paraffin