Glucocorticoid receptors in monocytes in type 1 diabetes mellitus.
Glucocorticoid receptor binding characteristics were investigated in 8 males with poorly controlled Type 1 diabetes mellitus and 14 healthy males. The cell type studied was monocytes, and a method for correction for heterogeneity in glucocorticoid binding in a mononuclear leucocyte population was introduced. The number of receptors and the dissociation constant KD were, respectively, 13,699 and 2.93 X 10(-8) mol/l for the control group and 15,788 and 2.75 X 10(-8) mol/l for diabetics (p greater than 0.05). In diabetics, KD correlated negatively with blood glucose (r = 0.762, p less than 0.05) indicating an increased sensitivity to cortisol at high blood glucose levels. In 6 of the diabetics and 7 of the control group, a simultaneous insulin receptor study was carried out. However, glucocorticoid receptor binding characteristics did not correlate with insulin receptor binding characteristics or with HbA1c. In conclusion, no major abnormalities in glucocorticoid receptor binding characteristics could be demonstrated in Type 1 diabetes mellitus.
Damm
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P
Hagedorn Research Laboratory, Gentofte, Denmark.
Binder
C
C
D000328:Adult; D003907:Dexamethasone / Q000378:metabolism; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000032:analysis*; D008214:Lymphocytes / Q000032:analysis; D008297:Male; D009000:Monocytes / Q000032:analysis* / Q000378:metabolism; D011965:Receptors, Glucocorticoid / Q000032:analysis* / Q000378:metabolism
D007328:Insulin; D011965:Receptors, Glucocorticoid; D003907:Dexamethasone