Laboratory diagnosis of pulmonary toxoplasmosis in patients with acquired immunodeficiency syndrome.
In four cases of pulmonary toxoplasmosis occurring in patients with acquired immunodeficiency syndrome, Toxoplasma sp. was discovered in bronchoalveolar-lavage fluid (three cases) and in lung biopsy specimen (one case) by using the following methods: direct examination of smears stained with eosine-methylene blue fast stain, indirect immunofluorescence assay, and inoculation of MRC5 fibroblast cell line in tissue culture.
Derouin
F
F
Laboratoire de Parasitologie, Hôpital Saint-Luuis, Paris, France.
Sarfati
C
C
Beauvais
B
B
Iliou
M C
MC
Dehen
L
L
Lariviere
M
M
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications*; D000372:Agglutination Tests; D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis; D001706:Biopsy; D001853:Bone Marrow / Q000469:parasitology; D001992:Bronchoalveolar Lavage Fluid; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D006801:Humans; D008168:Lung / Q000469:parasitology; D008174:Lung Diseases, Parasitic / Q000150:complications / Q000175:diagnosis*; D013183:Sputum / Q000469:parasitology; D014122:Toxoplasma / Q000276:immunology / Q000302:isolation & purification; D014123:Toxoplasmosis / Q000150:complications / Q000175:diagnosis*
D000913:Antibodies, Protozoan