title: Immunopharmacological actions of an extract isolated from inflamed skin of rabbits inoculated with Vaccinia virus (Neurotropin)--II. Restorative effect on immune responses through the recovery of IL-2 production in aging mice.
abstract: To examine the immunopharmacological actions of an extract isolated from inflamed skin of rabbits inoculated with Vaccinia virus (Neurotropin), its effect on the immune responses in aging BALB/c mice was examined. Neurotropin clearly restored the decreasing T-cell-dependent immune responses such as delayed-type hypersensitivity (DTH) response and plaque-forming cells (PFC) response to sheep red blood cells (SRBC) when administered i.p. from 13 months old (mo) to 16 mo. However, Neurotropin administration from 2 to 5 mo had no effect on the immune responses of young animals. Neurotropin administration from 13 to 16 mo restored not only the T-cell proliferation of spleen cells induced by concanavalin A (Con A) and phytohemagglutinin (PHA), but also the interleukin-2 (IL-2) production by spleen cells activated with Con A. However, Neurotropin did not affect the responsiveness of Con A-activated spleen cells to exogenous recombinant IL-2. An absence of suppressor cells capable of inhibiting the IL-2 production in the spleens was confirmed in the 16 mo mice. Neurotropin administration also restored IL-1 production by peritoneal macrophages stimulated with lipopolysaccharide (LPS). These results suggest that long-term administration of Neurotropin restores the decreasing T-cell-dependent immune responses through the recovery of IL-2 and in part IL-1 production, but not the responsiveness to IL-2 in aging BALB/c mice.
authors.affiliation: Department of Immunology and Allergy, Institute of Bio-Active Science, Nippon Zoki Pharmaceutical Company, Hyogo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yoshii
authors.forename: S
authors.initials: S
authors.lastname: Tatsumi
authors.forename: T
authors.initials: T
authors.lastname: Matsuoka
authors.forename: S
authors.initials: S
authors.lastname: Suehiro
authors.forename: J
authors.initials: J
authors.lastname: Konishi
authors.forename: Y
authors.initials: Y
authors.lastname: Yanagihara
authors.forename: S
authors.initials: S
authors.lastname: Negoro
authors.forename: S
authors.initials: S
authors.lastname: Kishimoto
mesh_terms: D000276:Adjuvants, Immunologic; D000375:Aging / Q000276:immunology*; D000818:Animals; D005260:Female; D006462:Hemolytic Plaque Technique; D006968:Hypersensitivity, Delayed; D007111:Immunity, Cellular / Q000187:drug effects; D007375:Interleukin-1 / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011134:Polysaccharides / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects*; D013997:Time Factors
chemical_list: D000276:Adjuvants, Immunologic; D007375:Interleukin-1; D007376:Interleukin-2; D011134:Polysaccharides; C010491:neurotropin