Protection of cotton rats against human respiratory syncytial virus by vaccination with a novel chimeric FG glycoprotein.
The cotton rat model of experimental human respiratory syncytial virus (RSV) infection was used to study the efficacy of FG, a novel chimeric glycoprotein which was expressed in insect cells using a baculovirus vector. FG contained the extracellular regions of the F (fusion) and G (attachment) glycoproteins of RSV. Vaccination with FG resulted in induction of neutralizing antibody and was correlated with protection of lung tissue from RSV challenge against both serogroup A and B virus strains. Both crude FG taken from supernatants of insect cells and affinity-purified FG were immunogenic and active against RSV. FG vaccination was effective by three routes of administration, following a single dose, and when administered with different adjuvants.
Brideau
R J
RJ
Upjohn Company, Kalamazoo, Michigan 49001.
Walters
R R
RR
Stier
M A
MA
Wathen
M W
MW
D000276:Adjuvants, Immunologic / Q000276:immunology; D000818:Animals; D003411:Arvicolinae; D004306:Dose-Response Relationship, Immunologic; D006023:Glycoproteins / Q000276:immunology; D007115:Immunization Schedule; D011993:Recombinant Fusion Proteins / Q000276:immunology; D012136:Respiratory Syncytial Viruses / Q000276:immunology*; D010253:Respirovirus Infections / Q000517:prevention & control*; D014612:Vaccines / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
D000276:Adjuvants, Immunologic; D006023:Glycoproteins; D011993:Recombinant Fusion Proteins; D014612:Vaccines; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D014765:Viral Vaccines