title: Effect of zinc deficiency on the appearance of two immunodominant protein antigens (32 kDa and 65 kDa) in culture filtrates of mycobacteria.
abstract: After growth of six strains of mycobacteria on Sauton medium in the absence of added Zn2+, cell yields were lowered, to between 22% and 67% of the yields obtained when Zn2+ (5 microM) was added. Two immunodominant proteins, named P64 and P32 (antigens of 62-65 kDa and 29-33 kDa, respectively) were abundant in culture filtrates after growth of mycobacteria. P64 was present at elevated concentrations (showing a 9- to 16-fold increase as a percentage of the total protein released) after Zn2+-deficient growth of five of the six strains studied; in Mycobacterium tuberculosis it represented 25% of all released proteins. However, little P64 was detected in culture filtrates of M. fortuitum and of M. phlei grown under Zn2+ deficiency, and in the latter there was no increase of P64 during Zn2+ deficiency.
authors.affiliation: Institut Pasteur du Brabant, Bruxelles, Belgium.
authors.forename: J
authors.initials: J
authors.lastname: De Bruyn
authors.forename: R
authors.initials: R
authors.lastname: Bosmans
authors.forename: J
authors.initials: J
authors.lastname: Nyabenda
authors.forename: J P
authors.initials: JP
authors.lastname: Van Vooren
mesh_terms: D000942:Antigens, Bacterial / Q000096:biosynthesis*; D001431:Bacteriological Techniques; D003470:Culture Media; D009161:Mycobacterium / Q000276:immunology*; D013045:Species Specificity; D015032:Zinc / Q000172:deficiency*
chemical_list: D000942:Antigens, Bacterial; D003470:Culture Media; D015032:Zinc