Identification and characterization of a cyanate permease in Escherichia coli K-12.
Escherichia coli contains an inducible enzyme, cyanase, that catalyzes the decomposition of cyanate into ammonia and bicarbonate. The gene encoding cyanase, cynS, was cloned and found to be on a DNA fragment that contained the lac operon. Characterization of a plasmid encoding cyanase indicated that a 26-kilodalton (kDa) protein of unknown function was also induced by cyanate (Y-C. Sung, D. Parsell, P.M. Anderson, and J.A. Fuchs, J. Bacteriol. 169:2639-2642, 1987). The gene encoding the 26-kDa protein was located between cynS and its promoter, indicating the existence of a cyn operon. The 26-kDa protein was identified as a cyanate permease that transports exogenous cyanate by active transport. E. coli was shown to contain a cyanate transport system that is energy dependent and saturable by cyanate.
Sung
Y C
YC
Department of Biochemistry, University of Minnesota, St. Paul 55108.
Fuchs
J A
JA
D000619:Aminohydrolases / Q000235:genetics / Q000378:metabolism*; D027321:Anion Transport Proteins; D019759:Carbon-Nitrogen Lyases; D003485:Cyanates / Q000494:pharmacology; D004790:Enzyme Induction; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins; D005796:Genes; D005798:Genes, Bacterial; D007700:Kinetics; D026901:Membrane Transport Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D008970:Molecular Weight; D010957:Plasmids; D015183:Restriction Mapping
D027321:Anion Transport Proteins; D003485:Cyanates; D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; C057329:cyanate permease, E coli; D000619:Aminohydrolases; C019953:cyanate hydrolase; D019759:Carbon-Nitrogen Lyases; C024835:potassium cyanate