title: Differential expression of lymphocyte function-associated antigen 1 (LFA-1) on epidermotropic and non-epidermotropic T-cell clones.
abstract: Various T-cell surface molecules are involved in T-cell adhesion, which is an essential requirement for epidermotropic migration of T cells. Our previous studies demonstrated that certain T-cell clones can migrate into the epidermis upon their intradermal inoculation into the footpads of recipient mice with relevant antigens, while other T-cell clones, despite their identical antigen specificities and functions, are non-epidermotropic. We therefore tested whether the differences in epidermotropism of these T cells could reside in the different levels of expression of T-cell surface molecules such as CD3, CD4, CD2, and lymphocyte function associated antigen 1 (LFA 1). The results of flow cytometric analysis showed that LFA-1 was preferentially expressed on the surface of epidermotropic T-cell clones, while non-epidermotropic T-cell clones were negative or very weakly positive for LFA-1 with one exception. After exposure to phorbol ester, epidermotropic clones with high levels of LFA-1 did not show any further up-regulation of LFA-1. In contrast, under identical conditions, significant up-regulation of LFA-1 was observed on non-epidermotropic T cells with low levels of LFA-1. However, even after exposure to phorbol ester, these T cells remained non-epidermotropic. These results suggest that the presence of high levels of LFA-1 on T cells is absolutely necessary for their epidermotropic migration, but its up-regulation is neither necessary nor sufficient to trigger the epidermotropic migration. High levels of LFA-1, regardless of cell activation, may be required to mediate stable cell adhesions leading to epidermotropic migration.
authors.affiliation: Department of Dermatology, Kyorin University School of Medicine, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Shiohara
authors.forename: N
authors.initials: N
authors.lastname: Moriya
authors.forename: C
authors.initials: C
authors.lastname: Gotoh
authors.forename: J
authors.initials: J
authors.lastname: Hayakawa
authors.forename: K
authors.initials: K
authors.lastname: Saizawa
authors.forename: H
authors.initials: H
authors.lastname: Yagita
authors.forename: M
authors.initials: M
authors.lastname: Nagashima
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000276:immunology*; D002465:Cell Movement; D002999:Clone Cells / Q000276:immunology*; D004817:Epidermis / Q000502:physiology; D005260:Female; D008213:Lymphocyte Activation; D016169:Lymphocyte Function-Associated Antigen-1; D051379:Mice; D008815:Mice, Inbred Strains; D016028:Receptors, Leukocyte-Adhesion / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000943:Antigens, Differentiation; D016169:Lymphocyte Function-Associated Antigen-1; D016028:Receptors, Leukocyte-Adhesion; D013755:Tetradecanoylphorbol Acetate