[Methods used in evaluating mutagenic and genotoxic properties of chemical compounds. II. The Ames test (an abridged version)].
The paper presents in vitro the technique developed by Ames et al (1.5), applied in screening tests for evaluations of mutagenic effects of chemical substances. As the experimental model in the test use histidine-dependent bacterial cells of Salmonella typhimurium series TA strains. Chemical substances biotransformation is carried out in the presence of microsomal activating system (fraction S9), mostly obtained from rat's liver. Mutagenic activity of chemical substances is measured by an induction of his- mutation reversion in S. typhimurium strains, expressed as number His+ of revertant colonies, as compared to number His+ of spontaneous revertant colonies of a given strain on Petri dishes containing minimal glucose agar after 48-72 hr incubation at 37 degrees C.
Janik-Spiechowicz
E
E
Z Zakładu Kancerogenezy Zawodowej Instytutu Medycyny Pracy w Lodzi.
D003470:Culture Media; D004121:Dimethyl Sulfoxide / Q000633:toxicity*; D066298:In Vitro Techniques; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens; D009154:Mutation; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics*; D013489:Suppression, Genetic / Q000187:drug effects*
D003470:Culture Media; D009153:Mutagens; D004121:Dimethyl Sulfoxide
Metodyki stosowane do oceny właściwości mutagennych i genotoksycznych zwiazków chemicznych. Cześć II. Płytkowy test Amesa (wersja skrócona).