title: Localization of functional centers on the prokaryotic ribosome: immuno-electron microscopy approach.
abstract: Immuno-electron microscopy studies on the morphology of the Escherichia coli ribosome and the topography of its functional centers are summarized. A three-dimensional model of the ribosome with indications of the mRNA-binding site, the peptidyl-transferase center, the EF-Tu and EF-G-binding sites and the tRNA-binding sites in given. Thus, the mutual arrangement of the centers corresponding to the main functional activities of the ribosome during the process of translation is presented.
authors.affiliation: Institute of Protein Research, Academy of Sciences of the USSR, Moscow Region.
authors.forename: A S
authors.initials: AS
authors.lastname: Spirin
authors.forename: V D
authors.initials: VD
authors.lastname: Vasiliev
mesh_terms: D001665:Binding Sites; D002477:Cells / Q000648:ultrastructure*; D008854:Microscopy, Electron; D010445:Peptide Elongation Factors / Q000378:metabolism; D010458:Peptidyl Transferases / Q000378:metabolism; D011387:Prokaryotic Cells / Q000378:metabolism / Q000648:ultrastructure*; D012333:RNA, Messenger / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D010445:Peptide Elongation Factors; D012333:RNA, Messenger; D012343:RNA, Transfer; D010458:Peptidyl Transferases