Reduction of the translation fidelity by kanamycin: effects on growth and mutant frequency in S. typhimurium TA102.
Kanamycin reduces the translation fidelity in prokaryotes. By read-through of the ochre stop codon (hisG428) of S. typhimurium TA102 enough functional enzyme is produced to allow the his- cells to form a dense background growth on the minimal agar plates. The influence on the revertant colony numbers is similar to the effect of histidine supplementation.
Gocke
E
E
Department of Toxicology, F. Hoffmann-La Roche and Co. Ltd., Basel, Switzerland.
D006639:Histidine / Q000494:pharmacology; D007612:Kanamycin / Q000633:toxicity*; D009152:Mutagenicity Tests; D009154:Mutation; D014176:Protein Biosynthesis / Q000187:drug effects*; D012486:Salmonella typhimurium / Q000235:genetics*; D013307:Streptomycin / Q000633:toxicity
D006639:Histidine; D007612:Kanamycin; D013307:Streptomycin