Molecular cloning of the recA analog from the marine fish pathogen Vibrio anguillarum 775.
The recA analog from Vibrio anguillarum 775 was isolated by complementation of recA mutations in Escherichia coli, and its protein product was identified. The recA analog promoted recombination between two partially deleted lactose operons, stimulated both spontaneous and mitomycin C-induced phage production in RecA- lambda lysogens, and restored near wild-type levels of resistance to UV radiation and methyl methanesulfonate.
Singer
J T
JT
Department of Microbiology, University of Maine, Orono 04469.
D000818:Animals; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005399:Fishes / Q000382:microbiology*; D005816:Genetic Complementation Test; D009154:Mutation; D009876:Operon; D011938:Rec A Recombinases / Q000235:genetics* / Q000302:isolation & purification / Q000528:radiation effects; D015183:Restriction Mapping; D014466:Ultraviolet Rays; D014733:Vibrio / Q000235:genetics* / Q000472:pathogenicity / Q000528:radiation effects
D011938:Rec A Recombinases