A production of Dermatophagoides farinae allergen-specific IgE and Ig(G + M) antibodies by cultured peripheral blood mononuclear cells of patients with extrinsic bronchial asthma.
Using a modified reverse hemolytic plaque assay, we measured in vitro production of Dermatophagoides farinae allergen-specific IgE and Ig(G + M) antibodies by peripheral blood mononuclear cells in 19 patients with extrinsic bronchial asthma. Circulating the allergen-specific IgE was estimated by means of the Phadebas radioallergosorbent test system. We found a significant difference in the production of the allergen-specific IgE antibody by the cells between asthmatic patients with positive and negative circulating the allergen-specific IgE.
Chao
C L
CL
First Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
Tamura
G
G
Mue
S
S
Takishima
T
T
D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000276:immunology*; D000818:Animals; D039741:Antigens, Dermatophagoides; D001249:Asthma / Q000276:immunology*; D005260:Female; D006462:Hemolytic Plaque Technique; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis / Q000096:biosynthesis / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis / Q000096:biosynthesis / Q000276:immunology; D007075:Immunoglobulin M / Q000032:analysis / Q000096:biosynthesis / Q000276:immunology; D007136:Immunoglobulins / Q000096:biosynthesis*; D007564:Japan; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D008875:Middle Aged; D008925:Mites / Q000276:immunology; D012882:Skin Tests
D000485:Allergens; D039741:Antigens, Dermatophagoides; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D007073:Immunoglobulin E