Plasmodium falciparum antigens associated with membrane structures in the host erythrocyte cytoplasm.
Hybridomas were made from mice immunized with plasma membranes from erythrocytes infected with Plasmodium falciparum. Among the monoclonal antibodies produced, a series reacted with antigens in the host cell cytoplasm. Immunoelectron microscopy, along with indirect fluorescent antibody double labeling experiments, were used to further localize the antigens to membrane structures (presumably Maurer's clefts) in the erythrocyte cytoplasm. The epitopes thus localized are found on three parasite proteins (20 kDa, 29 kDa, and 45 kDa) and one parasite glycoprotein (45 kDa). They are likely to be part of a transport system for the parasite.
Stanley
H A
HA
Agouron Institute, La Jolla, CA 92037.
Langreth
S G
SG
Reese
R T
RT
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000953:Antigens, Protozoan / Q000032:analysis* / Q000276:immunology; D015153:Blotting, Western; D004910:Erythrocyte Membrane / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology / Q000469:parasitology* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000276:immunology; D007425:Intracellular Membranes / Q000276:immunology*; D051379:Mice; D008854:Microscopy, Electron; D010963:Plasmodium falciparum / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D006023:Glycoproteins