Formation of functional inter-species hybrid photosynthetic complexes in Rhodobacter capsulatus.
A Rhodobacter capsulatus mutant strain deficient in all pigment-binding peptides and hence incapable of photosynthetic growth was genetically complemented with a plasmid-borne copy of the Rhodobacter sphaeroides puf operon. Hybrid reaction centers composed of R. sphaeroides L and M and R. capsulatus H subunits assembled in vivo, and host cells were photosynthetically competent. Light-harvesting complex B875, also encoded by the R. sphaeroides puf operon, was present along with the hybrid reaction center. These cells emitted fluorescence, however, indicating an impairment in energy transduction.
Zilsel
J
J
Department of Microbiology, University of British Columbia, Vancouver, Canada.
Lilburn
T G
TG
Beatty
J T
JT
D001419:Bacteria / Q000235:genetics*; D002734:Chlorophyll / Q000235:genetics; D002736:Chloroplasts; D003001:Cloning, Molecular; D005816:Genetic Complementation Test; D045342:Light-Harvesting Protein Complexes; D008970:Molecular Weight; D010788:Photosynthesis; D045322:Photosynthetic Reaction Center Complex Proteins; D010940:Plant Proteins / Q000235:genetics; D010957:Plasmids; D012242:Rhodobacter sphaeroides / Q000235:genetics; D013045:Species Specificity; D013057:Spectrum Analysis
D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D010940:Plant Proteins; D002734:Chlorophyll