title: Comparison of different Fasciola hepatica antigens and their use to detect infection in sheep by an enzyme immunoassay.
abstract: Soluble antigens of adult Fasciola hepatica were extracted from homogenized parasites and purified with gel chromatography on Sephadex G-200. Six peaks were obtained, and the second highest in molecular weight showed highest correspondence in a microplate enzyme immuno assay with parasite metabolite antigens. Optimal coating antigen concentration was 5 micrograms/ml and sample dilution 1:20. Total running time of assay was 3 hours. Positive and negative sheep sera was obtained from a group of experimentally infected tracer lambs.
authors.forename: G
authors.initials: G
authors.lastname: Cecchini
authors.forename: O B
authors.initials: OB
authors.lastname: Kasali
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis*; D000947:Antigens, Helminth / Q000276:immunology*; D005210:Fasciola hepatica / Q000276:immunology*; D005211:Fascioliasis / Q000175:diagnosis / Q000662:veterinary*; D007124:Immunoenzyme Techniques; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth