A Candida albicans mutant conditionally defective in sterol 14 alpha-demethylation.
A conditional sterol 14 alpha-demethylation mutant of Candida albicans was isolated whose defect was dependent both on the growth temperature and on the culture medium used. Under restrictive conditions, this mutant showed a deficiency in hyphal growth and an increased susceptibility to diverse chemicals, in accordance with the phenotypes of the already known non-conditional mutants of 14 alpha-demethylation. In addition, the mutant was shown to have a conditional defect in respiration. The impaired respiration was also seen in the non-conditional mutants.
Shimokawa
O
O
Department of Microbiology, School of Dentistry, Kyushu University, Fukuoka, Japan.
Nakayama
H
H
D002176:Candida albicans / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D002849:Chromatography, Gas; D003470:Culture Media; D009154:Mutation; D010101:Oxygen Consumption; D013261:Sterols / Q000096:biosynthesis*; D013696:Temperature
D003470:Culture Media; D013261:Sterols