Inhibition by mannose of in vitro colonization of chicken small intestine by Salmonella typhimurium.
The in vitro adherence of [3H]thymidine-labeled Salmonella typhimurium isolates to the small intestine of one-day-old chickens was investigated. Bacteria were screened for mannose sensitivity and mannose-resistance binding properties. Type 1 fimbriae positive strains adhered significantly better than Type 2 fimbriae-negative strains. Adherence was significantly (P less than .05) inhibited by D-mannose, methyl-alpha-D-mannoside, arabinose, and galactose. Adherence was both time and temperature dependent. These findings suggest that the small intestine of the chicken has receptors for bacteria with Type 1 fimbriae. The function of the receptors is dependent on a mannose moiety. Bacteria adhered better to fresh intestine cells than to cells held overnight at 4 C. Thus, adherence was dependent upon a metabolically active host cell. The in vitro adherence assay may further be used to study the interaction of bacteria with chicken enterocytes.
Oyofo
B A
BA
U.S. Department of Agriculture, Veterinary Toxicology and Entomology Research Laboratory, College Station, Texas 77840.
Droleskey
R E
RE
Norman
J O
JO
Mollenhauer
H H
HH
Ziprin
R L
RL
Corrier
D E
DE
DeLoach
J R
JR
D000818:Animals; D001089:Arabinose / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000187:drug effects*; D002645:Chickens / Q000382:microbiology*; D005690:Galactose / Q000494:pharmacology; D007421:Intestine, Small / Q000382:microbiology* / Q000648:ultrastructure; D008297:Male; D008358:Mannose / Q000494:pharmacology*; D008766:Methylmannosides / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D008871:Microvilli / Q000382:microbiology; D012486:Salmonella typhimurium / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure
D008766:Methylmannosides; C008466:methylmannoside; D001089:Arabinose; D008358:Mannose; D005690:Galactose