Monoclonal islet cell surface antibody dependent cellular cytotoxicity mediated by rat splenocytes with the RIN cell line as target.
A new method is described for testing monoclonal islet cell surface antibodies for their ability to mediate cellular immune effector mechanisms (ADCC). For the first time an IgM mediated cellular cytotoxicity is demonstrated. By using the RIN cell line as target the method was reproducible and easy to perform. The significance of such definition of monoclonal ICSA's to experimental diabetes research is discussed.
Hulinsky
I
I
Central Institute of Diabetes Gerhardt Katsch, Karlsburg, GDR.
Ziegler
B
B
Cabrera
E
E
Schlosser
M
M
Witt
S
S
Ziegler
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000920:Antibody-Dependent Cell Cytotoxicity / Q000187:drug effects*; D001323:Autoantibodies / Q000276:immunology*; D002460:Cell Line; D002478:Cells, Cultured; D007111:Immunity, Cellular / Q000187:drug effects; D007070:Immunoglobulin A / Q000276:immunology; D007515:Islets of Langerhans / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D011917:Rats, Inbred Lew; D013154:Spleen / Q000166:cytology* / Q000502:physiology
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D007070:Immunoglobulin A; C024211:islet cell antibody