Comparison of enzyme-linked immunosorbent assay (ELISA) with dot hybridization using 32P- or 2-acetylaminofluorene (AAF)-labelled cDNA probes for the detection and characterization of beet necrotic yellow vein virus.
Beet Necrotic Yellow Vein Virus (BNYVV) was detected by enzyme-linked immunosorbent assay (ELISA) and RNA/DNA dot hybridization using either radiolabelled or non-radioactive probes. Dot hybridization specifically distinguished isolates that could not be distinguished by ELISA. The detection thresholds for ELISA, hybridization with non-radioactive probes and hybridization with radiolabelled probes were 2 ng, 0.2 ng, 0.02 ng of purified virus, respectively. Dot hybridization with non-radioactive probes could be performed on crude infected beet root extracts, thus providing a useful tool for monitoring BNYVV infection and for routine testing in plant breeding programs.
Sakamoto
H
H
Laboratoire des Sondes Froides, Institut Pasteur, Paris, France.
Lemaire
O
O
Merdinoglu
D
D
Guesdon
J L
JL
D015073:2-Acetylaminofluorene; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D009693:Nucleic Acid Hybridization; D010761:Phosphorus Radioisotopes; D010935:Plant Diseases; D010942:Plant Viruses / Q000302:isolation & purification*; D012367:RNA, Viral / Q000032:analysis; D014675:Vegetables
D015342:DNA Probes; D004279:DNA, Viral; D010761:Phosphorus Radioisotopes; D012367:RNA, Viral; D015073:2-Acetylaminofluorene