title: Human ret proto-oncogene mapped to chromosome 10q11.2.
abstract: Using cosmid clones derived from human ret protooncogene as probes, we determined its chromosome localization by fluorescence in situ hybridization. Two overlapping clones, cret-1 and -2, which were cloned using the most 5' part of human ret proto-oncogene cDNA, hybridized to chromosome 10q11.2. Sixty-three and 52% of the grains obtained by cret-1 and -2, respectively, were localized to the same site. No other specific hybridization site was observed. From these data, we assigned the site of ret proto-oncogene to chromosome 10q11.2, where the possible locus responsible for multiple endocrine neoplasia type 2A (MEN2A) was mapped by linkage analysis using interstitial retinol binding protein cDNA and D10S5. These findings suggest that ret proto-oncogene might be a suitable probe for approaching the MEN2A locus.
authors.affiliation: Carcinogenesis Division, National Cancer Center Research Institute, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Ishizaka
authors.forename: F
authors.initials: F
authors.lastname: Itoh
authors.forename: T
authors.initials: T
authors.lastname: Tahira
authors.forename: I
authors.initials: I
authors.lastname: Ikeda
authors.forename: T
authors.initials: T
authors.lastname: Sugimura
authors.forename: J
authors.initials: J
authors.lastname: Tucker
authors.forename: A
authors.initials: A
authors.lastname: Fertitta
authors.forename: A V
authors.initials: AV
authors.lastname: Carrano
authors.forename: M
authors.initials: M
authors.lastname: Nagao
mesh_terms: D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10; D003001:Cloning, Molecular; D003360:Cosmids; D029721:Drosophila Proteins; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000166:cytology; D009693:Nucleic Acid Hybridization; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D051096:Proto-Oncogene Proteins c-ret; D011519:Proto-Oncogenes; D020794:Receptor Protein-Tyrosine Kinases; D012016:Reference Values; D015183:Restriction Mapping
chemical_list: D029721:Drosophila Proteins; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D011505:Protein-Tyrosine Kinases; D051096:Proto-Oncogene Proteins c-ret; D020794:Receptor Protein-Tyrosine Kinases; C416649:Ret protein, Drosophila