Escherichia coli recA deletion strains that are highly competent for transformation and for in vivo phage packaging.
I describe the construction of a variety of Escherichia coli recA deletion strains designed to facilitate molecular cloning. These recA deletion strains permit the efficient cloning of foreign inserts carried in plasmid, phage, cosmid, phasmid (phage-plasmid hybrid) or phosmid (phage-cosmid hybrid) vectors.
Kurnit
D M
DM
Howard Hughes Medical Institute, Department of Pediatrics, University of Michigan Medical Center, Ann Arbor 48109-0650.
D003001:Cloning, Molecular / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics*; D005822:Genetic Vectors; D011938:Rec A Recombinases / Q000235:genetics*; D014169:Transformation, Bacterial
D011938:Rec A Recombinases