[The development of a method of nitrosoguanidine inactivation in the mutagenic treatment of bacteria].
A simple method for the inactivation of nitrosoguanidine, a mutagenic agent permitting the simplification and acceleration of the procedure used for obtaining bacterial mutants, is described. This method is based on the addition of unithiol to nitrosoguanidine solution to a final concentration of 0.25-0.5% and the subsequent normalization of the pH of the medium with 0.1 N NaOH solution.
Kulichenko
A N
AN
Popov
Iu A
IuA
D001419:Bacteria / Q000187:drug effects*; D001431:Bacteriological Techniques; D002614:Chelating Agents / Q000494:pharmacology; D004347:Drug Interactions; D009154:Mutation; D009604:Nitrosoguanidines / Q000037:antagonists & inhibitors*; D009930:Organic Chemicals; D013885:Thiosulfates / Q000494:pharmacology; D014494:Unithiol / Q000494:pharmacology
D002614:Chelating Agents; D009604:Nitrosoguanidines; D009930:Organic Chemicals; D013885:Thiosulfates; C061848:Trialon B; D014494:Unithiol; C017717:sodium thiosulfate
Razrabotka metoda inaktivatsii nitrozoguanidina pri mutagennoĭ obrabotke bakteriĭ.