Biosynthesis of the peroxisomal dihydroxyacetone synthase from Hansenula polymorpha in Saccharomyces cerevisiae induces growth but not proliferation of peroxisomes.
The DAS gene of Hansenula polymorpha was expressed in Saccharomyces cerevisiae under the control of different promoters. The heterologously synthesized dihydroxyacetone synthase (DHAS), a peroxisomal enzyme in H. polymorpha, shows enzymatic activity in baker's yeast. The enzyme was imported into the peroxisomes of S. cerevisiae not only under the appropriate physiological conditions for peroxisome proliferation (oleic acid media), but also in glucose-grown cells where it induced the enlargement of the few peroxisomes present. This growth process was not accompanied by an increase in the number of microbodies, which suggests a separate control mechanism for peroxisomal proliferation.
Gödecke
A
A
Institut für Mikrobiologie, Universität Düsseldorf, Federal Republic of Germany.
Veenhuis
M
M
Roggenkamp
R
R
Janowicz
Z A
ZA
Hollenberg
C P
CP
D000429:Alcohol Oxidoreductases / Q000096:biosynthesis / Q000235:genetics; D019880:Aldehyde-Ketone Transferases; D015153:Blotting, Western; D002458:Cell Fractionation; D015966:Gene Expression Regulation, Fungal; D007150:Immunohistochemistry; D008830:Microbodies / Q000201:enzymology* / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron; D010843:Pichia / Q000201:enzymology / Q000235:genetics* / Q000648:ultrastructure; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000648:ultrastructure; D004718:Saccharomycetales / Q000235:genetics*; D014158:Transcription, Genetic; D014166:Transferases / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D014170:Transformation, Genetic
D000429:Alcohol Oxidoreductases; C029293:methanol oxidase; D014166:Transferases; D019880:Aldehyde-Ketone Transferases; C031222:formaldehyde transketolase