[Methodologic aspects of immunoperoxidase technics].
In attempt to standardize immunoperoxidase tests many partial methodological experiments were performed and the results discussed. The proved processing included purification of peroxidase conjugates, sequential administering in double indirect immunoperoxidase method (double sandwich). A high level dilution of primary antibodies and lengthening of incubation were recommended. Silicagel and vaseline rim round sections were found advantageous for improving adhesion of paraffin sections.
Capek
P
P
Rozprimová
L
L
Kolárová
M
M
D007124:Immunoenzyme Techniques
Metodické aspekty imunoperoxidázových technik.