Cloning and characterization of an Escherichia coli gene, pcnB, affecting plasmid copy number.
A gene, pcnB, affecting the copy number of ColE1-related plasmids has been cloned and mapped to 3.6 min on the Escherichia coli chromosome between panD and fhu. The gene encodes a previously undescribed 48 kD protein. Several independently isolated mutants exhibiting the same phenotype, reduced copy number, have been shown to be pcnB-.
March
J B
JB
Department of Molecular Biology, University of Edinburgh.
Colloms
M D
MD
Hart-Davis
D
D
Oliver
I R
IR
Masters
M
M
D000483:Alleles; D000668:Ampicillin Resistance; D001426:Bacterial Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D008970:Molecular Weight; D009154:Mutation; D010641:Phenotype; D010957:Plasmids; D014176:Protein Biosynthesis; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014161:Transduction, Genetic
D001426:Bacterial Proteins