Initial phase of infection with Tyzzer's organism in cultured mouse hepatocytes.
The initial phase of infection with Tyzzer's organisms in cultured mouse hepatocytes was observed using indirect immunofluorescence (IF) and a plaque assay. The organisms adhered poorly to aldehyde- or acetone-fixed cells, but once adhered to host cells, whether methanol-fixed or unfixed, they were not removed by methanol or acetone. By the IF as well as the plaque assay, both of which discriminated intra- and extracellularly located organisms, the number of cell-associated organisms increased linearly up to 3 h post-inoculation. The number of intra-cellular organisms increased rapidly in the first 1 h, followed by linear increase at a much lower rate. Similar results were obtained by the plaque assay.
Kawamura
S
S
Department of Biomedical Science, Faculty of Agriculture, University of Tokyo, Japan.
Mitsuoka
T
T
Fujiwara
K
K
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001407:Bacillus / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology*; D001422:Bacterial Adhesion; D002478:Cells, Cultured; D005260:Female; D005404:Fixatives / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D007074:Immunoglobulin G / Q000276:immunology; D008099:Liver / Q000382:microbiology*; D051379:Mice; D008813:Mice, Inbred ICR
D000907:Antibodies, Bacterial; D005404:Fixatives; D007074:Immunoglobulin G