Comparison of a 550,000 dalton cartilage matrix glycoprotein in cartilage from immature and mature dogs.
Cartilage matrix glycoprotein is a disulfide bonded 550,000 dalton protein found in cartilage and in the vitreous of the eye. Immunofluorescence studies using a specific antiserum to this glycoprotein have previously demonstrated a difference between the topographic distribution of staining for cartilage matrix glycoprotein in cartilage from immature and mature animals. Using a polyclonal antiserum to cartilage matrix glycoprotein, we studied this protein in cartilage from normal immature and mature dogs to determine if it changes with maturation. Cartilage matrix glycoprotein from immature canine cartilage migrates as a doublet, with apparent molecular weights of 100-116,000, regardless of its state of reduction, and is immunologically cross reactive with cartilage matrix glycoprotein from adult canine cartilage. Cartilage matrix glycoprotein from mature canine cartilage is disulfide bonded before reduction with 2-mercaptoethanol and its 116,000 Da subunit migrates as a single band after reduction.
Fife
R S
RS
Department of Medicine, Indiana University School of Medicine Indianapolis, 46223.
D000375:Aging / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D000906:Antibodies; D000941:Antigens / Q000032:analysis*; D002358:Cartilage, Articular / Q000032:analysis*; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D005136:Eye Proteins / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D008970:Molecular Weight
D000906:Antibodies; D000941:Antigens; D005136:Eye Proteins; C050677:cartilage-related protein (vitreous body)