[Construction of a family of artificial genes of human insulin].
Using oligonucleotide-directed mutagenesis and chemical-enzymatic DNA synthesis, genes for A and B insulin chains, C-peptide and Tyr-C-peptide have been constructed starting from synthetic gene for human proinsulin synthesized earlier. The genes for human preproinsulin, mini-proinsulin, single-chain insulin and their modifications were also synthesized. The constructions obtained were cloned in plasmid vectors.
Efimov
V A
VA
Buriakova
A A
AA
Pashkova
I N
IN
Polushin
N N
NN
Chakhmakhcheva
O G
OG
D000595:Amino Acid Sequence; D004926:Escherichia coli / Q000235:genetics; D005813:Genes, Synthetic; D005818:Genetic Engineering; D006801:Humans; D007328:Insulin / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D011384:Proinsulin / Q000235:genetics; D011498:Protein Precursors / Q000235:genetics
D007328:Insulin; D011498:Protein Precursors; C013737:preproinsulin; D011384:Proinsulin
Konstruirovanie semeĭstva iskusstvennykh genov insulina cheloveka.