Comparison of plasma renin and prorenin in healthy infants and children as determined with an enzymatic method and a new direct immunoradiometric assay.
We compared plasma active and inactive renin (prorenin) concentrations and activities in infants and children, as measured with a newly available direct immunoradiometric assay kit for active renin and a widely used enzymatic renin assay (plasma renin activity). The study was performed in 57 healthy infants and children under steady-state conditions and in eight subjects after orthostatic stimulation. Our study; (i) reports concentrations for active and inactive renin determined by the immunoradiometric assay in normal infants and children; (ii) confirms, by use of the immunoradiometric assay, the previously described decrease in plasma active and inactive renin, as measured by the enzymatic assay, with increasing age; and (iii) shows close correlations between the results obtained by immunoradiometric and enzymatic assays for active and inactive renin in normal infants and children.
Blazy
I
I
Department of Physiology, Hopital Necker-Enfants Malades, Paris, France.
Guillot
F
F
Laborde
K
K
Dechaux
M
M
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D004792:Enzyme Precursors / Q000097:blood*; D006801:Humans; D007158:Immunologic Techniques; D007223:Infant; D007231:Infant, Newborn; D011863:Radioimmunoassay; D012016:Reference Values; D012083:Renin / Q000097:blood*
D004792:Enzyme Precursors; D012083:Renin