A new beta-lactam-binding protein derived from penicillin-binding protein 3 of Escherichia coli.
In this paper we describe a new beta-lactam-binding protein from the cell envelope of Escherichia coli. It can be detected in cells grown at either 37 or 42 degrees C in medium containing glucose but not in cells grown at 30 degrees C. This novel component has an apparent molecular size that is 2.0 kilodaltons larger than that of penicillin-binding protein 3 and is derived from the latter through a divalent-cation-mediated process probably catalyzed by components located in the periplasmic space. The significance of this protein with regard to regulation of the amount of functional penicillin-binding protein 3 in the cell is discussed.
Prats
R
R
Instituto de Biologia Molecular, Universidad Autonoma, Madrid, Spain.
Gomez
M
M
Pla
J
J
Blasco
B
B
Ayala
J A
JA
D000217:Acyltransferases / Q000302:isolation & purification*; D000667:Ampicillin / Q000378:metabolism; D001426:Bacterial Proteins; D002352:Carrier Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins; D006602:Hexosyltransferases / Q000302:isolation & purification* / Q000378:metabolism; D007700:Kinetics; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000302:isolation & purification* / Q000378:metabolism; D002267:Muramoylpentapeptide Carboxypeptidase; D009994:Osmolar Concentration; D046915:Penicillin-Binding Proteins; D046928:Peptidoglycan Glycosyltransferase; D010458:Peptidyl Transferases / Q000302:isolation & purification* / Q000378:metabolism; D011485:Protein Binding; D013816:Thermodynamics
D001426:Bacterial Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; C478704:FtsI protein, E coli; D009097:Multienzyme Complexes; D046915:Penicillin-Binding Proteins; D000667:Ampicillin; D000217:Acyltransferases; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D046928:Peptidoglycan Glycosyltransferase; D002267:Muramoylpentapeptide Carboxypeptidase