Experimental Salmonella typhimurium infections in rats. I: Influence of thymus on the course of infection.
The course of experimentally induced Salmonella typhimurium infection was studied in three groups of inbred LEW rats: homozygous +/+, athymic rnu/rnu and isogeneic thymus-grafted rnu/rnu rats. In the first experiment the animals were inoculated intraperitoneally with 10(8) bacteria and all animals became severely septicemic and died within a week of inoculation, irrespective of presence or absence of thymus. In the second experiment the animals were inoculated with 10(6) bacteria, and both euthymic and thymus-grafted animals responded with high titres of anti bacterial antibodies while these were very low in the athymic nude animals. Polyclonal antibody production was only observed in the euthymic animals and only regarding IgG. Athymic rats were not able to clear the infection, while the thymus-grafted animals reacted like euthymic rats: Very few animals housed the bacteria four weeks after inoculation, and no bacteria could be detected after four months. Immunohistochemical studies of lymphoid organs revealed that the infection caused a drop in the percentages of T non-helper cells, indicating low suppressor activity. The study shows that athymic nude rats are well suited for studies of infectious diseases in immune deficient hosts, and that whole neonatally isogeneic thymus grafting is a good way of reconstituting these animals immunologically.
Hougen
H P
HP
University Institute of Forensic Medicine, Rigshospitalet, Copenhagen, Denmark.
Jensen
E T
ET
Klausen
B
B
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D000943:Antigens, Differentiation / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007958:Leukocyte Count; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008297:Male; D051381:Rats; D011923:Rats, Nude / Q000276:immunology; D012481:Salmonella Infections, Animal / Q000097:blood / Q000276:immunology*; D012486:Salmonella typhimurium; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology*
D000907:Antibodies, Bacterial; D000943:Antigens, Differentiation; D007074:Immunoglobulin G; D007075:Immunoglobulin M