[Structure of the lipopolysaccharide/human plasma low density lipoprotein complex. 31P-NMR and fluorescence spectroscopy studies].
Complexes of Salmonella typhimurium lipopolysaccharide toxin (LPS) with low density lipoproteins (LDL) containing various amounts of LPS were prepared in vitro. The 31P-NMR spectra showed that in the LDL-LPS complexes as well as in native LDL all phosphate groups of phospholipids are accessible to the paramagnetic shift reagent, Pr3+. Besides, the low frequency mobility of phospholipid phosphates in the complex is diminished. It was supposed that the phospholipid molecules in the LDL/LPS complex as in native LDL form a monolayer structure on the surface of LDL. The intrinsic fluorescence spectra of tryptophan residues of the apoprotein (apo B-100) revealed that the incorporation of LPS molecules into LDL particles is accompanied by minor changes in the conformation and orientation of the apo B molecule. As a result of these changes, certain fragments become exposed to a more hydrophilic environment and become more accessible to fluorescence quenchers. The use of charged (I-, Cs+) and uncharged (acrylamide) quenchers permitted to identify in the apo B molecule different tryptophan residues, some of which are localized in the vicinity of negatively charged groups, whereas others are neighbouring positively charged groups. It is suggested that the LPS molecules incorporated into LDL particle do not screen the apo B molecule to such an extent that it would hinder the LDL/LPS complex binding to apo B/E cellular receptors.
Viktorov
A V
AV
Gladkaia
E M
EM
Iurkiv
V A
VA
D006801:Humans; D008070:Lipopolysaccharides / Q000032:analysis*; D008077:Lipoproteins, LDL / Q000097:blood*; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D012486:Salmonella typhimurium / Q000032:analysis; D013050:Spectrometry, Fluorescence; D014364:Tryptophan / Q000032:analysis
D008070:Lipopolysaccharides; D008077:Lipoproteins, LDL; D014364:Tryptophan
Struktura kompleksa lipopolisakharid/lipoprotein nizkoĭ plotnosti plazmy cheloveka. Izuchenie metodami 31P-IaMR i fluorestsentnoĭ spektroskopii.