[Synthesis of oligoribonucleotides with the use of RNA polymerases of E. coli and immobilized synthetic DNA-templates].
A new approach to synthesis of oligoribonucleotides is suggested, based on transcription by E. coli RNA polymerase of synthetic immobilized DNA-templates with AUG as primer. The approach has been experimentally verified by synthesis of two oligonucleotides, viz., a RNA fragment of the fr phage (16 nucleotides long) and a RNA fragment of the tickborne encephalitis virus (18 nucleotides long). Fraction of the synthesized RNA fragments in the whole nucleotide material is about 20%. The templates can be used repeatedly. Sequences of the oligoribonucleotides were confirmed. Advantages of this approach and its usefulness for SP6 DNA-dependent RNA polymerase are discussed.
Denisova
L Ia
LIa
Zagrebel'nyy
S N
SN
Pustoshilova
N M
NM
Ven'iaminova
A G
AG
Gorn
V V
VV
D001345:Autoradiography; D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology*; D008969:Molecular Sequence Data; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D013698:Templates, Genetic
D009843:Oligoribonucleotides; D012321:DNA-Directed RNA Polymerases
Sintez oligoribonukleotidov s ispol'zovaniem RNK-polimerazy E. coli i immobilizovannykh sinteticheskikh DNK-matrits.