Effects of GTP gamma S and fMet-Leu-Phe on rat PMN phospholipase C (PLC) activity.
The PLC activities associated with the cytosol and plasma membrane subcellular fractions of rat PMN were tested for sensitivity to stimulation by GTP gamma S and/or fMet-Leu-Phe. PMN plasma membrane PLC was stimulated 10-20% by 50 microM GTP gamma S (p less than 0.02). fMet-Leu-Phe alone had no effect on the plasma membrane PLC activity, but addition of fMet-Leu-Phe and GTP gamma S together stimulated PLC activity by 20-30% (p less than 0.05). Neither GTP gamma S nor fMet-Leu-Phe had any significant effect on the cytosolic PLC activity. These data demonstrate the presence of a PLC activity in rat PMN plasma membranes that is sensitive to stimulation by GTP gamma S and fMet-Leu-Phe.
Mackin
W M
WM
Medical Products Department, E. I. DuPont de Nemours & Co., Inc., Wilmington, DE 19898.
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D003600:Cytosol / Q000187:drug effects / Q000201:enzymology; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology*; D051381:Rats; D013873:Thionucleotides / Q000494:pharmacology*; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors*
D013873:Thionucleotides; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D009240:N-Formylmethionine Leucyl-Phenylalanine; D006160:Guanosine Triphosphate; D010738:Type C Phospholipases