Further evidence for photoinduced genotoxicity of dictamnine as shown by prophage induction.
Photobiological activity of dictamnine, a furoquinoline alkaloid, to induce lytic phage development in a lysogen of Escherichia coli was measured as a line of evidence for the photoinduced genotoxicity. Since dictamnine forms the monoadducts to DNA but not the diadducts (DNA cross-links) by photoirradiation, the photobiological activity was compared with that of a cross-linking agent 5-methoxypsoralen, a structural analog, on the basis of relative quantum yield. The activity of dictamnine with respect to both phage induction and photoinduced lethal activity was weaker than the psoralen derivative. Any lethal DNA damage including monoadducts and diadducts of 5-methoxypsoralen appeared to contribute to prophage induction at the same level of efficiency.
Fujita
H
H
Department of Molecular Biology, Tokai University School of Medicine, Kanagawa-ken, Japan.
Kakishima
H
H
D000078223:5-Methoxypsoralen; D000470:Alkaloids / Q000494:pharmacology*; D001435:Bacteriophages / Q000502:physiology*; D004249:DNA Damage; D004269:DNA, Bacterial / Q000187:drug effects*; D004926:Escherichia coli / Q000187:drug effects / Q000502:physiology*; D008730:Methoxsalen / Q000494:pharmacology; D010777:Photochemistry; D011804:Quinolines; D014466:Ultraviolet Rays; D014775:Virus Activation / Q000187:drug effects*
D000470:Alkaloids; D004269:DNA, Bacterial; D011804:Quinolines; D000078223:5-Methoxypsoralen; C026398:dictamnine; D008730:Methoxsalen