title: [Use of nonradioactive probes to detect the gene for 3-aminoside-acetyltransferase type IV in strains of Escherichia coli and Salmonella spp].
abstract: Forty-three isolates of Escherichia coli, Salmonella dublin and S. thyphimurium of animal origin were studied by colony hybridization using an intragenic probe specific for the gene encoding 3-aminoglycoside acetyltransferase type IV, which confers resistance to apramycin and gentamicin. Non-radioactive biotinylated and sulphonated DNA probes were used. No false-positive results were found and the positive results obtained were in agreement with those previously revealed with radioactive probes.
authors.affiliation: Ministère de l'Agriculture, Direction générale de l'Alimentation, Lyon, France.
authors.forename: B
authors.initials: B
authors.lastname: Perrin
authors.forename: M
authors.initials: M
authors.lastname: Solsona
authors.forename: E
authors.initials: E
authors.lastname: Chaslus-Dancla
authors.forename: J L
authors.initials: JL
authors.lastname: Martel
mesh_terms: D000123:Acetyltransferases / Q000235:genetics*; D015342:DNA Probes; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012475:Salmonella / Q000235:genetics*
chemical_list: D015342:DNA Probes; D000123:Acetyltransferases; C019681:aminoglycoside acetyltransferase
vernacular_title: Utilisation de sondes non radioactives pour la détection du gène de la 3-aminoside-acétyltransférase type IV chez des souches de Escherichia coli et Salmonella spp.