title: Crystallization and preliminary X-ray studies of an aspartate aminotransferase mutant from Escherichia coli.
abstract: Mutant aspartate aminotransferase V39L (Val39 replaced by Leu) from Escherichia coli has been crystallized into a monoclinic cell from a polyethylene glycol solution (pH 7.5) by vapor diffusion. The space group and the unit cell dimensions have been determined using a precession camera, a CAD4 diffractometer and a Nicolet Xentronics area detector to be P2(1) with a = 86.8 A, b = 79.9 A, c = 89.4 A, beta = 118.74 degrees. The crystals diffract to better than 2.3 A and are suitable for X-ray structure analysis.
authors.affiliation: Department of Structural Biology, University of Basel, Switzerland.
authors.forename: J
authors.initials: J
authors.lastname: Jäger
authors.forename: E
authors.initials: E
authors.lastname: Köhler
authors.forename: P
authors.initials: P
authors.lastname: Tucker
authors.forename: U
authors.initials: U
authors.lastname: Sauder
authors.forename: Z
authors.initials: Z
authors.lastname: Housley-Markovic
authors.forename: I
authors.initials: I
authors.lastname: Fotheringham
authors.forename: M
authors.initials: M
authors.lastname: Edwards
authors.forename: M
authors.initials: M
authors.lastname: Hunter
authors.forename: K
authors.initials: K
authors.lastname: Kirschner
authors.forename: J N
authors.initials: JN
authors.lastname: Jansonius
mesh_terms: D001219:Aspartate Aminotransferases; D003460:Crystallization; D004926:Escherichia coli / Q000201:enzymology*; D008958:Models, Molecular; D009154:Mutation; D011487:Protein Conformation; D014961:X-Ray Diffraction
chemical_list: D001219:Aspartate Aminotransferases