title: Delta sleep-inducing peptide (DSIP)-like immunoreactivity in gut: coexistence with known peptide hormones.
abstract: Delta sleep-inducing peptide-like immunoreactivity (DSIP-LI) has previously been demonstrated in brain neurons and in endocrine cells of the pituitary and the adrenal medulla. By means of three different antisera against synthetic DSIP we now describe the occurrence and distribution of DSIP-LI in several gut endocrine cells. The human gut was the richest source, where DSIP-LI was located in gastrin/CCK, secretin and PYY/glicentin cells. The rat and pig gut harbour a moderate number of immunoreactive cells in the antral mucosa but in the intestines DSIP-LI-containing cells were very few. By radioimmunoassay, the concentration of DSIP-LI was determined in extracts of various gut regions from man, pig and rat. The highest concentrations were found in all human specimens compared with corresponding samples in the pig and rat. In all three species, high-performance liquid chromatography revealed a single peak of DSIP-like material with approximately the same retention time as DSIP 3-9. Taken together, the present results provide evidence for the presence of DSIP-LI in gut endocrine cells in man, pig and rat; the human gut seems to be the richest source of DSIP-like peptides.
authors.affiliation: Department of Medical Cell Research, Malmö General Hospital, University of Lund, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Bjartell
authors.forename: R
authors.initials: R
authors.lastname: Ekman
authors.forename: J
authors.initials: J
authors.lastname: Hedenbro
authors.forename: K
authors.initials: K
authors.lastname: Sjölund
authors.forename: F
authors.initials: F
authors.lastname: Sundler
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003106:Colon / Q000032:analysis* / Q000166:cytology / Q000473:pathology; D003701:Delta Sleep-Inducing Peptide / Q000032:analysis* / Q000276:immunology; D004386:Duodenum / Q000032:analysis* / Q000166:cytology / Q000473:pathology; D005455:Fluorescent Antibody Technique; D005768:Gastrointestinal Hormones / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007082:Ileum / Q000032:analysis* / Q000166:cytology / Q000473:pathology; D007124:Immunoenzyme Techniques; D008875:Middle Aged; D011706:Pyloric Antrum / Q000032:analysis* / Q000166:cytology / Q000473:pathology; D011863:Radioimmunoassay; D051381:Rats; D013552:Swine
chemical_list: D005768:Gastrointestinal Hormones; D003701:Delta Sleep-Inducing Peptide