Partial purification and characterization of phospholipase C from Yersinia enterocolitica.
About 34% of the strains of Yersinia enterocolitica isolated from raw milk were found to produce lecithinase. A selected strain produced phospholipase C at 22 degrees C and 37 degrees C; production was optimum at 37 degrees C in the stationary phase (14-16 h). A decrease in phospholipase C activity at various storage temperatures (-5 degrees C, 4 degrees C, 37 degrees C) was also observed, although the enzyme was active over a wide range of temperature (5-65 degrees C) and pH (3.5-7.5). The phospholipase C was partially purified by ammonium sulphate precipitation and Sephadex column chromatography, and characterized.
Toora
S
S
Department of Microbiology, Panjab University, Chandigarh, India.
Singh
G
G
Dhar
S
S
Singh
A D
AD
Tiwari
R P
RP
Singh
G
G
D000818:Animals; D015373:Bacterial Typing Techniques; D002020:Buffaloes; D004591:Electrophoresis, Polyacrylamide Gel; D005516:Food Microbiology; D008892:Milk / Q000382:microbiology*; D010738:Type C Phospholipases / Q000032:analysis / Q000302:isolation & purification*; D015008:Yersinia enterocolitica / Q000201:enzymology*
D010738:Type C Phospholipases