Gene function identified by interspecific transformation.
Aspergillus nidulans is able to utilize 2-pyrrolidinone as a nitrogen source while two related Aspergillus species, A. niger and A. terreus, cannot. Mutations in the lamA gene of A. nidulans prevent growth on 2-pyrrolidinone. A plasmid (pLAM7) has been isolated containing the A. nidulans lamA gene and a divergently transcribed adjacent gene of unknown function. Transformation of A. terreus with subclones of pLAM7 showed that both genes are essential for the utilization of a new nitrogen source, 2-pyrrolidinone, in that species. The previously unidentified gene has been designated lamB.
Katz
M E
ME
Department of Genetics, University of Melbourne, Parkville, Victoria, Australia.
Hynes
M J
MJ
D001230:Aspergillus / Q000235:genetics* / Q000378:metabolism; D001233:Aspergillus nidulans / Q000235:genetics / Q000378:metabolism; D001234:Aspergillus niger / Q000235:genetics / Q000378:metabolism; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D005800:Genes, Fungal; D009154:Mutation; D010957:Plasmids; D011760:Pyrrolidinones / Q000378:metabolism; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014170:Transformation, Genetic
D011760:Pyrrolidinones