Bacterial adherence of group A streptococci to mucosal surfaces.
It is now recognized that bacteria bind to and colonize mucosal surfaces in a highly selective manner. After the organisms penetrate the nonspecific mechanical and cleansing forces, ligands (or adhesins) on the surface of the bacteria interact in a lock-and-key (or induced fit) fashion with complementary receptors on mucosal surfaces of the host. The adhesins are usually composed of proteins in the form of fimbriae or fibrillae and the receptors of glycolipids or glycoproteins. In group-A streptococci the adhesin, lipoteichoic acid (LTA), is anchored to a protein(s) on the surface of the bacterial cells and interacts through its lipid moiety with fibronectin molecules deposited on and bound to the epithelial cells. In an attempt to locate the region of fibronectin recognized by LTA and group-A streptococci, fibronectin was cleaved with thermolysin and the fragment mixture absorbed with Staphylococcus aureus or Streptococcus pyogenes. Staphylococci adsorbed several high molecular weight fragments as well as a 28- and a 23-kdalton fragment, whereas S. pyogenes cells absorbed only the 28-kdalton fragment completely. The adsorbtion of the fragments by S. pyogenes was blocked by LTA. Antibodies raised against a synthetic peptide copying the NH2 terminus of fibronectin reacted in a Western blot with the 28-kdalton fragment, indicating that S. pyogenes and its LTA react with the NH2-terminal region of fibronectin at a site distinct from that of S. aureus. Our findings are consistent with the idea that LTA mediates the attachment of group-A streptococci to fatty acid-binding sites of fibronectin deposited on mucosal epithelial cells.
Beachey
E H
EH
Veterans Administration Medical Center, Memphis, Tenn.
Courtney
H S
HS
D001422:Bacterial Adhesion; D001665:Binding Sites; D004847:Epithelial Cells; D005353:Fibronectins; D008070:Lipopolysaccharides / Q000378:metabolism*; D009092:Mucous Membrane; D013297:Streptococcus pyogenes / Q000472:pathogenicity*; D013682:Teichoic Acids / Q000378:metabolism*
D005353:Fibronectins; D008070:Lipopolysaccharides; D013682:Teichoic Acids; C009900:lipoteichoic acid