Studies of humoral and cell-mediated immunity to peptides shared by HLA-27.1 and Klebsiella pneumoniae nitrogenase in ankylosing spondylitis.
One-hundred and twenty-four patients with spondylarthropathies were studied for antibodies to the peptides from HLA-B27.1 and Klebsiella pneumoniae nitrogenase which share a QTDRED hexamer sequence. Of 60 male Norwegian ankylosing spondylitis (AS) patients 23.3% showed positive ELISA reactivity for B27.1 peptide compared with 4% of Norwegian male controls (P less than 0.10). This difference was not observed among patients and controls from New Mexico. All patients with anti-B27.1 antibody were HLA-B27+. Antibody to B27.1 peptide was present in 20% of normal female controls with at least one previous pregnancy. No female control without previous pregnancy showed positive anti-B27.1 peptide reactivity. Anti-Klebsiella peptide antibody was neither significantly elevated in AS nor correlated with anti-B27.1 peptide antibody. Significant migration inhibition by these peptides was not observed in AS or normal controls. The possible influence of epitope conformation, rather than sequence homology, in potentially cross-reacting determinants shared by bacterial antigens and human Class I molecules requires further study.
Tsuchiya
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N
Department of Medicine, University of Florida, Gainesville 32610.
Husby
G
G
Williams
R C
RC
D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000961:Antilymphocyte Serum / Q000096:biosynthesis; D002464:Cell Migration Inhibition; D005260:Female; D015235:HLA-B Antigens / Q000276:immunology*; D015796:HLA-B27 Antigen; D006801:Humans; D007111:Immunity, Cellular; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009591:Nitrogenase / Q000276:immunology*; D010455:Peptides / Q000276:immunology* / Q000494:pharmacology; D013167:Spondylitis, Ankylosing / Q000201:enzymology / Q000276:immunology*
D000907:Antibodies, Bacterial; D000961:Antilymphocyte Serum; D015235:HLA-B Antigens; D015796:HLA-B27 Antigen; D010455:Peptides; D009591:Nitrogenase