Isolation and expression in Saccharomyces cerevisiae of a gene encoding an alpha-amylase from Schwanniomyces castellii.
A gene (SWA1) encoding an alpha-amylase activity from Schwanniomyces castellii has been cloned and expressed, via yeast cloning vector YEp13, in Saccharomyces cerevisiae. By using a riboprobe which is internal to the SWA1 gene, a 1.55 kb transcript was detected in the poly(A)+ RNA from both Sw. castellii and a S. cerevisiae clone harboring the SWA1 gene. This transcript should, therefore, correspond to the SWA1 gene. In addition, the DNA strand determining the alpha-amylase activity has been defined. Transcription of the SWA1 gene appears to be highly regulated in Sw. castellii, whereas it is constitutive in the S. cerevisiae harboring this gene.
Abarca
D
D
Centro de Biología Molecular (CSIC-UAM), Universidad Autónoma, Madrid, Spain.
Fernández-Lobato
M
M
Claros
M G
MG
Jiménez
A
A
D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D015870:Gene Expression; D005800:Genes, Fungal; D007700:Kinetics; D010957:Plasmids; D011994:Recombinant Proteins / Q000378:metabolism; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D004718:Saccharomycetales / Q000201:enzymology / Q000235:genetics*; D014158:Transcription, Genetic; D000516:alpha-Amylases / Q000235:genetics* / Q000378:metabolism
D004271:DNA, Fungal; D011994:Recombinant Proteins; D000516:alpha-Amylases