[Analysis of distribution of S. cerevisiae cells for protein content using cell cycle models].
Histograms of cell distributions according to protein content obtained by means of flow cytofluorometry characterize the physiological state of the population as a whole and permit to calculate the velocity of protein accumulation in the cell in the course of the cell cycle. Dependence of population heterogeneity on culturing conditions is considered. Mathematical analysis of histograms of continuous cultures of S. cerevisiae is carried out at dilution rates 0.4 hours-1 and 0.05 hours-1. Calculations are carried out on condition that the protein content in the cell rises a) exponentially and b) linearly in the course of the cell cycle. At low growth rate (0.05 hours-1) the distribution is bimodal and therefore it is highly informative. The assumption concerning linear accumulation of the protein allows good approximation of the experimental distributions by the theoretical ones.
Ivanitskaia
Iu G
IuG
Matylevich
N P
NP
Petrikevich
S B
SB
D002453:Cell Cycle; D005434:Flow Cytometry; D005656:Fungal Proteins / Q000032:analysis*; D008433:Mathematics; D012441:Saccharomyces cerevisiae / Q000032:analysis / Q000166:cytology*
D005656:Fungal Proteins
Analiz razpredeleniĭ kletok S. cerevisiae po soderzhaniiu belka pri pomoshchi modeleĭ kletochnogo tsikla.