title: Direct HLA typing by rosetting with immunomagnetic beads coated with specific antibodies.
abstract: Paramagnetic monodisperse polymer beads were coated with purified human hybridoma antibodies specific for polymorphic HLA determinants. The mAb-coated beads bound specifically to cells from individuals with the relevant HLA type. The rosettes formed by beads and cells were isolated with a magnet. Rosette formation was evaluated microscopically and used as criterion for positive typing. By this rosette assay, fast and reliable HLA typing of whole blood was possible.
authors.affiliation: Institute of Medical Biology, University of Tromsø, Norway.
authors.forename: T
authors.initials: T
authors.lastname: Hansen
authors.forename: K
authors.initials: K
authors.lastname: Hannestad
mesh_terms: D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D006681:HLA-D Antigens; D015395:Histocompatibility Antigens Class I; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D007158:Immunologic Techniques; D012397:Rosette Formation
chemical_list: D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D006681:HLA-D Antigens; D015395:Histocompatibility Antigens Class I