title: Expression of Yb1 glutathione S-transferase using a baculovirus expression system.
abstract: A full-length cDNA clone was isolated for rat liver Yb1 glutathione S-transferase (EC 2.5.1.18). The coding sequence of Yb1 cDNA was inserted into a baculovirus vector for infection of Spodoptera frugiperda (SF9) cells. The enzymatically active recombinant Yb1 glutathione S-transferase protein has a native molecular weight of 42,000 daltons (by molecular sieve chromatography), a subunit molecular weight of 26,500 daltons (by SDS-polyacrylamide gel electrophoresis), a pI of 8.4 and an extinction coefficient E1%280 of 5.6 +/- 0.4.
authors.affiliation: Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, Republic of China.
authors.forename: J C
authors.initials: JC
authors.lastname: Hsieh
authors.forename: L F
authors.initials: LF
authors.lastname: Liu
authors.forename: W L
authors.initials: WL
authors.lastname: Chen
authors.forename: M F
authors.initials: MF
authors.lastname: Tam
mesh_terms: D000818:Animals; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D005982:Glutathione Transferase / Q000235:genetics* / Q000378:metabolism; D007304:Insect Viruses / Q000235:genetics*; D010957:Plasmids; D051381:Rats; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D011994:Recombinant Proteins; D004247:DNA; D005982:Glutathione Transferase