B lymphocyte response as an indicator of acute renal transplant rejection. II. Pretransplant and posttransplant B cell responses of m mitogen and donor cell-stimulated cultures.
In a prospective study we investigated the association of kidney graft rejection with pre- and posttransplant B cell responses in vitro after stimulation with pokeweed mitogen, Staphylococcus aureus Cowan I (SAC I), or donor lymphocytes. B cell differentiation was assessed in a reverse hemolytic plaque assay. Elevated pretransplant PWM- or SAC I-stimulated B cell responses were found to define patients with a high incidence of rejection episodes in the first 30 days posttransplant (P less than 0.005 and P less than 0.05, respectively). Elevated pretransplant donor cell-stimulated B cell responses were associated with a high risk of irreversible rejection (P less than 0.005). A posttransplant rise in donor cell-stimulated B cell responses was associated with an increase risk of a subsequent rejection crisis (P less than 0.05). Our data suggest that patients at risk of early rejection may identified by pretransplant testing of B cell responses.
Weimer
R
R
Department of Transplantation Immunology, University of Heidelberg, Federal Republic of Germany.
Daniel
V
V
Pomer
S
S
Opelz
G
G
D000921:Antibody-Producing Cells / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D006084:Graft Rejection; D006680:HLA Antigens / Q000276:immunology; D006462:Hemolytic Plaque Technique; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology*; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D011446:Prospective Studies; D013601:T-Lymphocytes / Q000276:immunology; D013997:Time Factors
D006680:HLA Antigens