The expression of enterotoxin A-B+ gene of V. cholerae in E. coli.
The cloning in E. coli of a cholerae toxin gene that is A-B+ has been successfully constructed by using DNA recombinant techniques. E. coli cells carrying the recombinant plasmid pMM-CTB have been shown to produce a large amount of CTB subunits which are secreted as extracellular proteins.
Ma
Q J
QJ
Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing.
Zhou
J G
JG
Yu
X Q
XQ
Liu
C X
CX
Xiong
L S
LS
Xu
Y Q
YQ
Huang
C F
CF
D000818:Animals; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D004274:DNA, Recombinant; D004768:Enterotoxins / Q000235:genetics* / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D011817:Rabbits; D011995:Recombination, Genetic; D014734:Vibrio cholerae / Q000235:genetics*
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D004768:Enterotoxins