title: Characterization of the increased binding of acetaldehyde to red blood cells in alcoholics.
abstract: Using equilibrium dialysis, we found that acetaldehyde, at the levels commonly occurring after ethanol ingestion, did not bind detectably to plasma proteins, but there was significant binding to red blood cells, more in alcoholics than in nonalcoholics. The binding to red blood cells was inhibited by pyridoxal phosphate and N-ethylmaleimide, suggesting adduction to amino and thiol groups. Binding kinetics were consistent with at least two sites. The one with the highest affinity for acetaldehyde corresponded to hemoglobin. Its affinity and Bmax were not changed in alcoholics, but these binding sites accounted for only 44% of the sites available in the red blood cells of alcoholics and 80% of those in controls. Moreover, this binding was not inhibited by N-ethylmaleimide. There was no detectable binding to red cell ghosts. Nonprotein binding was then assessed by changes in NADH produced by the addition of protein-free fractions of the cells to an alcohol dehydrogenase system in equilibrium; this revealed a second binder of lower affinity, larger capacity and with sensitivity to both inhibitors. This binding (possibly due to thiazolidine formation with cysteine) was enhanced in alcoholics, whose red blood cell cysteine content was doubled. Levels of red blood cell cysteine and acetaldehyde remained high for 2 weeks after withdrawal. Because of the prolonged persistence after withdrawal, these changes may provide new markers of alcoholism.
authors.affiliation: Alcohol Research and Treatment Center, Bronx Veterans Administration Medical Center, New York, NY.
authors.forename: R
authors.initials: R
authors.lastname: Hernández-Muñoz
authors.forename: E
authors.initials: E
authors.lastname: Baraona
authors.forename: I
authors.initials: I
authors.lastname: Blacksberg
authors.forename: C S
authors.initials: CS
authors.lastname: Lieber
mesh_terms: D000079:Acetaldehyde / Q000493:pharmacokinetics*; D000435:Alcoholic Intoxication / Q000097:blood; D000437:Alcoholism / Q000097:blood*; D001665:Binding Sites; D001667:Binding, Competitive; D004912:Erythrocytes / Q000378:metabolism*; D000431:Ethanol / Q000493:pharmacokinetics; D006801:Humans
chemical_list: D000431:Ethanol; D000079:Acetaldehyde