Optimisation of media for the culture of normal human epithelial cells from oesophageal mucosa.
A technique has been developed which allows growth and histology/cytochemistry of primary oesophageal mucosal explant cultures to be monitored over four weeks. The paper describes experiments designed to optimise media and culture conditions. The results suggest that optimal growth can be obtained in media containing RPMI 1640, and 10% horse or newborn calf serum. McCoy's 5A medium or Dulbecco's modified MEM could be substituted for RPMI but Iscove's serum-free medium or Ham's nutrient mixture inhibited growth or promoted fibroblast contamination. The essential additive appeared to be insulin while selenium was highly toxic to the cells. Hydrocortisone or EGF improved growth slightly under some conditions. Neither transferrin nor cholera toxin had any beneficial effect. None of the cell culture flask coating agents improved attachment or growth.
Mothersill
C
C
Radiobiology Unit, Saint Luke's Hospital, Dublin.
Cusack
A
A
Seymour
C B
CB
Hennessy
T P
TP
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media / Q000032:analysis; D004847:Epithelial Cells; D004947:Esophagus / Q000166:cytology*; D006801:Humans; D007328:Insulin / Q000032:analysis / Q000494:pharmacology; D009092:Mucous Membrane / Q000166:cytology
D003470:Culture Media; D007328:Insulin