title: An enlarged largest subunit of Plasmodium falciparum RNA polymerase II defines conserved and variable RNA polymerase domains.
abstract: We have isolated the gene encoding the largest subunit of RNA polymerase II from Plasmodium falciparum. The RPII gene is expressed in the asexual erythrocytic stages of the parasite as a 9 kb mRNA, and is present as a single copy gene located on chromosome 3. The P. falciparum RPII subunit is the largest (2452 amino acids) eukaryotic RPII subunit, and it contains enlarged variable regions that clearly separate and define five conserved regions of the eukaryotic RPII largest subunits. A distinctive carboxyl-terminal domain contains a short highly conserved heptapeptide repeat domain which is bounded on its 5' side by a highly diverged heptapeptide repeat domain, and is bounded on its 3' side by a long carboxyl-terminal extension.
authors.affiliation: Department of Microbiology, Dartmouth Medical School, Hanover, NH 03756.
authors.forename: W B
authors.initials: WB
authors.lastname: Li
authors.forename: D J
authors.initials: DJ
authors.lastname: Bzik
authors.forename: H M
authors.initials: HM
authors.lastname: Gu
authors.forename: M
authors.initials: M
authors.lastname: Tanaka
authors.forename: B A
authors.initials: BA
authors.lastname: Fox
authors.forename: J
authors.initials: J
authors.lastname: Inselburg
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D015698:Genomic Library; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010963:Plasmodium falciparum / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D012319:RNA Polymerase II / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
chemical_list: D046911:Macromolecular Substances; D015345:Oligonucleotide Probes; D004247:DNA; D012319:RNA Polymerase II