Monoamines in pancreatic islets of guinea pig, hamster, rat, and mouse determined by high performance liquid chromatography.
Previous studies on the occurrence of catecholamines and serotonin in pancreatic islets using various histochemical and chemical methods have given widely different results. We therefore performed a comparative analysis of these amines in whole pancreas and islet tissue from hamster, guinea pig, rat, and mouse by the use of high performance liquid chromatography. Whole pancreas of guinea pig, hamster, and rat had a norepinephrine concentration of approximately 1.1 mumol/kg of pancreatic wet weight. The mouse pancreas had less than one-half of that concentration. Epinephrine and dopamine concentrations were on the order of 0.02 mumol/kg of pancreatic wet weight in all four species. The serotonin concentration was 2.1 mumol/kg of pancreatic wet weight in the guinea pig pancreas and approximately 0.2 mumol/kg in the other three species studied. The catecholamine concentrations were much higher in the pancreatic islets than in the exocrine pancreas. Thus, the norepinephrine concentration was approximately 35 mumol/kg of islet wet weight in hamster islets and 5-10 mumol/kg in rat, guinea pig, and mouse islets. The epinephrine concentration in islet tissue ranged between 1 and 7 mumol/kg of islet wet weight and the dopamine concentration between 0.5 and 4 mumol/kg except for guinea pig islets (12 mumol/kg). The islet tissue in the mouse, rat, and guinea pig contained disproportionately more epinephrine and dopamine relative to norepinephrine than did the exocrine pancreas. Chemical sympathectomy (6-hydroxydopamine treatment) in the mouse reduced the norepinephrine and epinephrine concentrations in islet tissue to nondetectable levels, whereas the dopamine concentration was essentially unchanged, thus suggesting an extra-neuronal source of this amine in addition to its occurrence in adrenergic nerves.(ABSTRACT TRUNCATED AT 250 WORDS)
Lundquist
I
I
Department of Cell Biology, University of Linköping, Sweden.
Ahrén
B
B
Hansson
C
C
Håkanson
R
R
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006224:Cricetinae; D004298:Dopamine / Q000032:analysis*; D004837:Epinephrine / Q000032:analysis*; D005260:Female; D006168:Guinea Pigs; D007515:Islets of Langerhans / Q000032:analysis*; D008647:Mesocricetus; D051379:Mice; D008815:Mice, Inbred Strains; D009638:Norepinephrine / Q000032:analysis*; D010179:Pancreas / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000032:analysis*
D012701:Serotonin; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine