Analysis of fidelity mechanisms with eukaryotic DNA replication and repair proteins.
We are investigating the mechanisms for producing or avoiding errors during DNA synthesis catalyzed by DNA replication and repair proteins purified from eukaryotic sources. Using assays that monitor the fidelity of a single round of DNA synthesis in vitro, we have defined the error frequency and mutational specificity of the four classes of animal cell DNA polymerases (alpha, beta, delta, gamma), and the fidelity of an SV40 origin-dependent DNA replication complex in extracts of HeLa cells.
Kunkel
T A
TA
Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709.
Bebenek
K
K
Roberts
J D
JD
Fitzgerald
M P
MP
Thomas
D C
DC
D000818:Animals; D004256:DNA Polymerase I / Q000502:physiology; D004257:DNA Polymerase II / Q000502:physiology; D004258:DNA Polymerase III / Q000502:physiology; D004260:DNA Repair / Q000502:physiology*; D004261:DNA Replication / Q000502:physiology*; D004259:DNA-Directed DNA Polymerase / Q000502:physiology; D006801:Humans; D009154:Mutation
D004256:DNA Polymerase I; D004257:DNA Polymerase II; D004258:DNA Polymerase III; D004259:DNA-Directed DNA Polymerase