[BANA hydrolase and DNP peptidase activities in the ovulatory process of PMS-hCG treated immature rat].
To investigate the roles of collagenolytic enzymes in the ovulatory process of PMS-hCG treated immature female rats (22 days old), we measured the activities of two of them in the ovary by the using synthetic substrates alpha-N-benzoyl-DL-arginine-2-naphthylamide HCl (BANA) (a collagenolytic cathepsin) and dinitrophenol-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg OH (DNP peptide) (a neutral collagenase). BANA hydrolase activities significantly began to increase after the hCG injection, reaching a peak 8-9 hours later, and then decreased sharply 10 hours later. There was also a significant increase in DNP peptidase activities 7-10 hours after the hCG injection and a significant decrease 12 hours after the injection. The present study has shown that BANA hydrolase and DNP peptidase appear during the ovulatory process of PMS-hCG-treated immature female rats, and that their significant preovulatory increases are contributory to collagenolysis in follicle rupture.
Shibata
S
S
Department of Obstetrics and Gynecology, Kumamoto University Medical School, Kumamoto.
Ikuta
Y
Y
Furuki
Y
Y
Matsuura
K
K
Okamura
H
H
D000818:Animals; D056655:Cathepsin H; D002403:Cathepsins / Q000378:metabolism*; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D003094:Collagen / Q000378:metabolism; D003546:Cysteine Endopeptidases; D004140:Dinitrophenols / Q000378:metabolism*; D005260:Female; D006064:Gonadotropins, Equine / Q000494:pharmacology*; D009842:Oligopeptides / Q000378:metabolism*; D010053:Ovary / Q000187:drug effects / Q000201:enzymology* / Q000502:physiology; D010060:Ovulation / Q000187:drug effects; D010062:Ovulation Induction; D010447:Peptide Hydrolases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D006063:Chorionic Gonadotropin; D004140:Dinitrophenols; D006064:Gonadotropins, Equine; D009842:Oligopeptides; C024118:DNP-peptide; D003094:Collagen; D002403:Cathepsins; D010447:Peptide Hydrolases; D003546:Cysteine Endopeptidases; D056655:Cathepsin H; C534128:Ctsh protein, rat