Mechanisms of suppression of renal kallikrein activity in low renin essential hypertension and renoparenchymal hypertension.
The mechanism of suppression of renal kallikrein activity in low renin essential hypertensive and renoparenchymal hypertensive patients was investigated in this study. From Sephadex G-200 column chromatography studies, a single kallikrein peak was observed in both kallikrein radioimmunoassay and kininogenase activity in all samples from normal subjects, low renin essential hypertensive and renoparenchymal hypertensive patients, and in purified kallikrein solution. The enzyme-specific activity around the kallikrein peak in all urine samples from each group was significantly lower than that in purified kallikrein, and a significantly lower specific activity was found in both patient groups than was found in normal subjects. Moreover, it was also recognized that the specific activity of kallikrein decreased in all cases with the increase of the molecular weight of kallikrein, and this tendency was observed more obviously in the low renin essential hypertensive and renoparenchymal hypertensive patients than in the normal subjects. These results suggest the presence of a kallikrein-specific inhibitor with a low molecular weight in human urine, although the possibility of a variant form of kallikrein cannot be excluded.
Shimamoto
K
K
Second Department of Internal Medicine, Sapporo Medical College, Japan.
Masuda
A
A
Ando
T
T
Ura
N
N
Nakagawa
M
M
Mori
Y
Y
Nakagawa
H
H
Sakakibara
T
T
Ogata
H
H
Iimura
O
O
D005260:Female; D006801:Humans; D006973:Hypertension / Q000201:enzymology*; D006977:Hypertension, Renal / Q000201:enzymology; D007610:Kallikreins / Q000037:antagonists & inhibitors / Q000378:metabolism* / Q000652:urine; D007668:Kidney / Q000201:enzymology*; D008297:Male; D012083:Renin / Q000097:blood*
D007610:Kallikreins; D012083:Renin