title: nodO, a new nod gene of the Rhizobium leguminosarum biovar viciae sym plasmid pRL1JI, encodes a secreted protein.
abstract: The region of the Rhizobium leguminosarum biovar viciae Sym plasmid pRL1JI, responsible for the production and secretion of a previously described 50-kilodalton protein (R. A. de Maagd, C. A. Wijffelman, E. Pees, and B. J. J. Lugtenberg, J. Bacteriol. 170:4424-4427, 1988), was cloned and its nucleotide sequence was determined. A new nod gene, nodO, preceded by a poorly conserved nod box, was identified and its transcriptional start site was determined. Comparison of its predicted protein product with the N-terminal amino acid sequence of the isolated secreted protein showed that nodO is the structural gene of this protein, although the nucleotide sequence predicted a protein only 30,002 daltons in size. This comparison also showed that the secreted protein is not the product of N-terminal processing of a larger precursor. A conventional N-terminal signal sequence was not detected in the NodO protein. The NodO protein has significant homology with a part (residues 720 to 920) of the hemolysin protein (HlyA) of Escherichia coli. Analysis of the transcriptional regulation of the nodO gene revealed that, in contrast with other nod promoters in this species, activity of the nodO promoter is greatly enhanced in the presence of multiple copies of the nodD gene.
authors.affiliation: Department of Plant Molecular Biology, Leiden University, The Netherlands.
authors.forename: R A
authors.initials: RA
authors.lastname: de Maagd
authors.forename: A H
authors.initials: AH
authors.lastname: Wijfjes
authors.forename: H P
authors.initials: HP
authors.lastname: Spaink
authors.forename: J E
authors.initials: JE
authors.lastname: Ruiz-Sainz
authors.forename: C A
authors.initials: CA
authors.lastname: Wijffelman
authors.forename: R J
authors.initials: RJ
authors.lastname: Okker
authors.forename: B J
authors.initials: BJ
authors.lastname: Lugtenberg
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D002135:Calcium-Binding Proteins; D003001:Cloning, Molecular / Q000379:methods; D004926:Escherichia coli / Q000235:genetics; D044950:Flavanones; D005419:Flavonoids; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010957:Plasmids; D011487:Protein Conformation; D015183:Restriction Mapping; D012231:Rhizobium / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D002135:Calcium-Binding Proteins; D044950:Flavanones; D005419:Flavonoids; C060773:NodD protein, Bacteria; C062095:NodO protein, Rhizobium leguminosarum; C005273:naringenin