PGM-1: a transplantable murine leukemia of granulocyte-macrophage progenitor cells.
PGM-1 is a transplantable leukemia of C3H/HeJ mice growing as a population of undifferentiated blast cells with a predisposition to form subcutaneous tumors and to grow in lymphoid organs. Cell survival and proliferation in vitro are absolutely dependent on stimulation by hemopoietic growth factors, and up to 100% of tumor cells can form colonies of mature granulocytes and/or macrophages in semisolid cultures, the colonies containing no clonogenic cells. Most clonogenic cells in the leukemic population respond to stimulation by multi-colony-stimulating factor (IL-3) or GM-CSF, but some respond also to M-CSF, G-CSF, IL-4, IL-5, or IL-6. In their surface phenotype and proliferative characteristics in vitro, PGM-1 leukemic cells resemble normal granulocyte-macrophage progenitor cells, and the leukemia may be a useful model for human chronic myeloid leukemia.
Dührsen
U
U
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia.
Metcalf
D
D
Spangrude
G J
GJ
Nicola
N A
NA
D000818:Animals; D003115:Colony-Stimulating Factors / Q000032:analysis / Q000494:pharmacology; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000032:analysis / Q000494:pharmacology; D006412:Hematopoietic Stem Cells; D007377:Interleukin-3 / Q000032:analysis / Q000494:pharmacology; D007942:Leukemia, Experimental / Q000235:genetics / Q000473:pathology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009368:Neoplasm Transplantation; D014407:Tumor Cells, Cultured
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D007377:Interleukin-3; D016178:Granulocyte-Macrophage Colony-Stimulating Factor