title: Simultaneous detection of Escherichia coli heat-stable and heat-labile enterotoxin genes with a single RNA probe.
abstract: A single RNA probe was synthesized and used to detect simultaneously the methanol-soluble heat-stable enterotoxin and heat-labile enterotoxin genes in Escherichia coli strains. The results with the biotinylated or radioactive probe correlated 100% with the biological assay results for both toxins. The RNA probe detected the three known heat-stable enterotoxin A alleles.
authors.affiliation: Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235.
authors.forename: X
authors.initials: X
authors.lastname: Sáez-Llorens
authors.forename: L M
authors.initials: LM
authors.lastname: Guzman-Verduzco
authors.forename: S
authors.initials: S
authors.lastname: Shelton
authors.forename: J D
authors.initials: JD
authors.lastname: Nelson
authors.forename: Y M
authors.initials: YM
authors.lastname: Kupersztoch
mesh_terms: D000483:Alleles; D001427:Bacterial Toxins / Q000235:genetics*; D001710:Biotin; D004269:DNA, Bacterial / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D004768:Enterotoxins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D006801:Humans; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D015347:RNA Probes; D015183:Restriction Mapping
chemical_list: D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D015347:RNA Probes; C034151:heat stable toxin (E coli); D001710:Biotin; C027538:heat-labile enterotoxin, E coli