title: Mutations in ADE3 reduce the efficiency of the omnipotent suppressor sup45-2.
abstract: Mutations in a known yeast gene, ADE3, were shown to act as an antisuppressor, reducing the efficiency of the omnipotent suppressor, sup45-2. The ADE3 locus encodes the trifunctional enzyme C1-tetrahydrofolate synthase, which is required for the biosynthesis of purines, thymidylate, methionine, histidine, pantothenic acid and formylmethionyl-tRNA(fMet. The role of this enzyme in translational fidelity had not previously been suspected.
authors.affiliation: Department of Biological Sciences, University of Illinois, Chicago 60680.
authors.forename: J M
authors.initials: JM
authors.lastname: Song
authors.forename: S W
authors.initials: SW
authors.lastname: Liebman
mesh_terms: D000619:Aminohydrolases / Q000235:genetics*; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D005574:Formate-Tetrahydrofolate Ligase / Q000235:genetics*; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D008025:Ligases / Q000235:genetics*; D008754:Methylenetetrahydrofolate Dehydrogenase (NADP) / Q000235:genetics*; D009097:Multienzyme Complexes / Q000235:genetics*; D009154:Mutation; D010088:Oxidoreductases / Q000235:genetics*; D010641:Phenotype; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D013489:Suppression, Genetic; D014170:Transformation, Genetic
chemical_list: D004271:DNA, Fungal; D009097:Multienzyme Complexes; C021836:formyl-methenyl-methylenetetrahydrofolate synthetase; D010088:Oxidoreductases; D008754:Methylenetetrahydrofolate Dehydrogenase (NADP); D000619:Aminohydrolases; D008025:Ligases; D005574:Formate-Tetrahydrofolate Ligase