title: Periplasmic superoxide dismutases in Aquaspirillum magnetotacticum.
abstract: Aquaspirillum magnetotacticum MS-1 cells cultured microaerobically (dissolved O2 tension 1% of saturation), expressed proteins with superoxide dismutase (SOD) activity. The majority (roughly 95%) of total cell superoxide dismutase activity was located in the cell periplasm with little or no activity in the cell cytoplasm. Iron-type SOD (FeSOD) contributed 88% of the total activity activity detected, although a manganese-type SOD (MnSOD) was present in the periplasm as well. Cells cultured at a higher dissolved O2 tension (10% of saturation) expressed increased activity of the MnSOD relative to that of the FeSOD.
authors.affiliation: Department of Immunology and Microbiology, University of Michigan Medical School, Ann Arbor 48109-0620.
authors.forename: K A
authors.initials: KA
authors.lastname: Short
authors.forename: R P
authors.initials: RP
authors.lastname: Blakemore
mesh_terms: D002374:Catalase / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology; D007700:Kinetics; D008970:Molecular Weight; D010544:Peroxidases / Q000378:metabolism; D013045:Species Specificity; D013139:Spirillum / Q000201:enzymology*; D013482:Superoxide Dismutase / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D010544:Peroxidases; D002374:Catalase; D013482:Superoxide Dismutase