Additive stimulation of hepatic putrescine production by glucagon and insulin after partial hepatectomy in rats.
When rats received glucagon or insulin every 2 h after partial hepatectomy (Hx), hepatic putrescine content was increased above control levels at 6 and 12 h, respectively. When the two hormones were combined, the increased levels were additive. Hepatic ornithine decarboxylase activity was above control levels at 12 h after insulin treatment. Hepatic spermidine N1-acetyltransferase activity was enhanced at 6 h only when glucagon was dosed. Putrescine administration from 0 to 4 h or from 6 to 10 h increased hepatic DNA synthesis to similar levels 22 h after Hx. These results suggest that glucagon and insulin additively stimulate hepatic putrescine production after Hx. This may explain the cooperative stimulation of liver regeneration by both hormones.
Sato
Y
Y
First Department of Medicine, Faculty of Medicine, University of Japan.
Fujiwara
K
K
Ogata
I
I
Tomiya
T
T
Oka
H
H
D000123:Acetyltransferases / Q000378:metabolism; D000818:Animals; D004347:Drug Interactions; D005934:Glucagon / Q000494:pharmacology*; D006498:Hepatectomy; D007328:Insulin / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008115:Liver Regeneration; D008297:Male; D009955:Ornithine Decarboxylase / Q000378:metabolism; D011700:Putrescine / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D000099228:Diamine N-Acetyltransferase
D000123:Acetyltransferases; D005934:Glucagon; D007328:Insulin; D009955:Ornithine Decarboxylase; D011700:Putrescine; D000099228:Diamine N-Acetyltransferase