[The diagnosis of antileptospiral antibodies in human subjects by solid-phase immunoenzyme analysis].
The possibility of using the enzyme-linked immunosorbent assay (ELISA) for the diagnosis of leptospirosis has been shown. This method has proved to be more simple and sensitive than the leptospiral microagglutination and lysis test. The data on obtaining genus-specific leptospiral antigens are presented. As revealed in this study, the antigens obtained by the complex treatment of microbial cells with ultrasound and detergents show the maximum activity in ELISA. The optimum parameters of the ELISA system for the diagnosis of leptospirosis have been established.
Sergeev
A A
AA
Chernukha
Iu G
IuG
Shakhanina
K L
KL
Gol'denshteĭn
Z A
ZA
Potashnikova
E Ia
EIa
D000907:Antibodies, Bacterial / Q000032:analysis*; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000032:analysis / Q000302:isolation & purification; D001782:Blood Donors; D005069:Evaluation Studies as Topic; D006505:Hepatitis / Q000175:diagnosis; D006801:Humans; D007124:Immunoenzyme Techniques; D007919:Leptospira / Q000276:immunology*; D007922:Leptospirosis / Q000175:diagnosis*; D013588:Syphilis Serodiagnosis
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial
Diagnostika protivoleptospiroznykh antitel u liudeĭ metodom tverdofaznogo immunofermentnogo analiza.