Macrophage multinucleation is accompanied by the expression of new soluble and membrane antigens in mice.
Polyclonal antibodies were raised against mouse multinucleated giant cells to identify proteins specifically associated with multinucleated macrophages. A 115 kd membrane protein and a 160 kd soluble protein were detected by immunoprecipitation of radiolabeled giant cells and were not seen in mononucleated macrophages. This study demonstrated that giant cells express de novo synthesized proteins that are not present in macrophages and identified a new immunization technique to generate antibodies directed against cell surface components.
Vignery
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Department of Orthopaedics and Rehabilitation, Yale University School of Medicine, New Haven, Connecticut 06510.
D000818:Animals; D000906:Antibodies; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000378:metabolism*; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D008264:Macrophages / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D011233:Precipitin Tests
D000906:Antibodies; D008565:Membrane Proteins; D004247:DNA