title: The use of murine feeder cells in the cultivation of Plasmodium falciparum asexual blood stages.
abstract: Increased multiplication rates were observed in asexual erythrocytic stages of Plasmodium falciparum grown in the presence of a feeder-cell layer of mouse peritoneal wash cells (PWCs) using the candle-jar method of Trager and Jensen (1977). This held true for both new and established isolates of the parasite. When the PWC population was separated into adherent and non-adherent fractions, the adherent PWC population promoted an increase in parasite growth but the non-adherent population did not. An increase in parasite multiplication was not promoted by PWC-conditioned medium.
authors.affiliation: Wellcome Laboratories for Experimental Parasitology, Glasgow University, Bearsden, UK.
authors.forename: K R
authors.initials: KR
authors.lastname: Trenholme
authors.forename: R S
authors.initials: RS
authors.lastname: Phillips
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D003470:Culture Media; D005260:Female; D008297:Male; D051379:Mice; D010529:Peritoneal Cavity / Q000166:cytology*; D010963:Plasmodium falciparum / Q000254:growth & development*
chemical_list: D003470:Culture Media