Electrophoretic karyotype of the pathogenic yeast Cryptococcus neoformans.
The electrokaryotype of the pathogenic yeast Cryptococcus neoformans is described for the first time. Three different patterns were seen: (a) serotypes B and C (variety gattii) are similar and consist of nine chromosome mobility groups of greater than 580 kb; (b) serotype A (variety neoformans) revealed eight chromosome-like groups greater than 700 kb; (c) serotype D (the second serotype of variety neoformans) not only differs from those described above, but each D isolate tested showed a different distribution of bands. The discrepancy, and the importance of electrokaryotyping as a taxonomic tool, are discussed.
Polacheck
I
I
Department of Clinical Microbiology, The Hebrew University-Hadassah Medical School, Hadassah Medical Center, Jerusalem, Israel.
Lebens
G A
GA
D003454:Cryptococcus / Q000235:genetics*; D003455:Cryptococcus neoformans / Q000145:classification / Q000235:genetics*; D004271:DNA, Fungal / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D007621:Karyotyping; D012703:Serotyping
D004271:DNA, Fungal