title: [Cloning of chloroplast psbA and rbcL-genes from cotton Gossipium hirsutum].
abstract: The fragments of cotton Gossipium hirsutum c.v. 108-f chloroplast genome were cloned in Escherichia coli cells. The cloned psbA and rbcL genes have been selected using the heterologous probes from spinach. The preliminary attempts to clone the complete psbA gene in pUC19 vector failed, probably, due to the toxicity of its product to Escherichia coli cells, and its 5'- and 3'-ends were cloned separately. Reconstruction experiments revealed that while the complete psbA gene was unable to be stably inherited by Escherichia coli cells, its structural part lacking the promoter region could be readily cloned in the bacterial cells.
authors.forename: T N
authors.initials: TN
authors.lastname: Ul'masov
authors.forename: M K
authors.initials: MK
authors.lastname: Gulov
authors.forename: K A
authors.initials: KA
authors.lastname: Aliev
authors.forename: V M
authors.initials: VM
authors.lastname: Andrianov
authors.forename: E S
authors.initials: ES
authors.lastname: Piruzian
mesh_terms: D002736:Chloroplasts; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D003368:Gossypium / Q000235:genetics*; D015183:Restriction Mapping
chemical_list: D004247:DNA
vernacular_title: Klonirovanie khloroplastnykh psbA i rbcL-genov khlopchatnika Gossipium hirsutum.