title: Analysis of mucosal bladder leucocyte subpopulations in patients treated with intravesical Bacillus Calmette-Guerin.
abstract: Immunohistochemical techniques were used to investigate leucocyte subpopulations in the bladders of patients with superficial transitional cell carcinoma treated with BCG Pasteur. Leucocyte subsets were enumerated with a panel of monoclonal antibodies which included CD3, CD4, CD8, TQ1, Leu7, CD15, HLA-DR, CD25, CD22. We demonstrated in the bladders of patients treated with BCG a particular lymphocyte population; the major subset was an inducer (CD4+, TQ1-) which was activated (CD25+, HLA-DR+) and associated with polymorphonuclear eosinophils. There was neither inducer of suppression nor major cytotoxic/suppressive subsets. CD8+ and NK cells could not be the primary mediators of BCG activity. These data supported the hypothesis of a helper T lymphocyte activity associated with lymphokine production and activation of effector killer cells.
authors.affiliation: Department of Pathology, Antoine Béclère Hospital, Clamart, France.
authors.forename: M
authors.initials: M
authors.lastname: Peuchmaur
authors.forename: G
authors.initials: G
authors.lastname: Benoit
authors.forename: A
authors.initials: A
authors.lastname: Vieillefond
authors.forename: A
authors.initials: A
authors.lastname: Chevalier
authors.forename: G
authors.initials: G
authors.lastname: Lemaigre
authors.forename: E D
authors.initials: ED
authors.lastname: Martin
authors.forename: A
authors.initials: A
authors.lastname: Jardin
mesh_terms: D001500:BCG Vaccine / Q000627:therapeutic use*; D002295:Carcinoma, Transitional Cell / Q000473:pathology / Q000628:therapy*; D006801:Humans; D007124:Immunoenzyme Techniques; D013601:T-Lymphocytes / Q000473:pathology*; D001743:Urinary Bladder / Q000473:pathology*; D001749:Urinary Bladder Neoplasms / Q000473:pathology / Q000628:therapy*
chemical_list: D001500:BCG Vaccine