Constitutive expression of a native human interferon-alpha 1 gene in Escherichia coli.
1. A plasmid for constitutive expression of the human interferon-alpha 1 (hIFN-alpha 1) gene in Escherichia coli is constructed on the basis of the cloning plasmid pBR322 using a strong synthetic promoter, synthetic ribosome binding site and a native hIFN-alpha 1 gene excised from a chromosomal clone. 2. The yield of recombinant hIFN-alpha 1 from E. coli LE392 cells transformed with the expression plasmid pJP1R9-hIFN-alpha 1 is evaluated to be 2-6 x 10(7) U/l bacterial culture for metabolic shaker and 6-8 x 10(7) U/l for fermentor.
Ivanov
I
I
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.
Markova
N
N
Bachvarov
D
D
Alexciev
K
K
Saraffova
A
A
Maximova
V
V
Tsaneva
I
I
Markov
G
G
D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D007370:Interferon Type I / Q000096:biosynthesis* / Q000235:genetics; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins; D015183:Restriction Mapping
D007370:Interferon Type I; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins