Down regulation of the mercury resistance operon by the most promoter-distal gene merD.
The effect of the merD gene on the expression of the mer operon was determined from the rates of accumulation of merA-lacZ fusion protein in the presence and absence of an active merD gene in trans. In the presence of the merD gene, beta-galactosidase activity was 2- to 4-fold lower. The merD gene was cloned in a T7 promoter expression vector and the MerD protein product was visualized by autoradiography.
Nucifora
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G
Department of Microbiology and Immunology, University of Illinois College of Medicine, Chicago 60680.
Silver
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S
Misra
T K
TK
D000595:Amino Acid Sequence; D001483:Base Sequence; D015536:Down-Regulation / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D007763:Lac Operon; D008628:Mercury / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009154:Mutation; D009876:Operon; D010641:Phenotype; D011401:Promoter Regions, Genetic
D008628:Mercury