title: Identification of an anti-A and anti-B blood group glycosyltransferase antibody after incompatible bone marrow transplant.
abstract: The occurrence of a potent antibody against plasmatic A and B glycosyltransferase activities has been characterized in a patient (blood group A1) transplanted with a bone marrow from a blood group O donor. A and B glycosyltransferases were purified to near homogeneity from plasma of A1 and B blood-group individuals. The half-maximal inhibition of both enzymes was obtained at 1 to 2 micrograms/mL of the post-transplant IgG fraction, prepared by protein A-sepharose chromatography. A and B glycosyltransferases were also recognized by the post-transplant IgG fraction after sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by electrophoretic transfer to nitrocellulose membranes.
authors.affiliation: Instituto de Bioquimica CSIC-UCM, Facultad de Farmacia, Madrid, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Mojena
authors.forename: L
authors.initials: L
authors.lastname: Boscá
mesh_terms: D000017:ABO Blood-Group System / Q000276:immunology*; D001787:Blood Group Incompatibility / Q000097:blood / Q000201:enzymology*; D016026:Bone Marrow Transplantation; D004591:Electrophoresis, Polyacrylamide Gel; D006602:Hexosyltransferases / Q000097:blood* / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D007074:Immunoglobulin G / Q000302:isolation & purification; D007518:Isoantibodies / Q000032:analysis* / Q000096:biosynthesis
chemical_list: D000017:ABO Blood-Group System; D007074:Immunoglobulin G; D007518:Isoantibodies; D006602:Hexosyltransferases; C018660:asparagine-N-glycosyltransferase