title: Transforming growth factor release by human tumor cell lines and their interactions with other growth factors.
abstract: We have studied the production of transforming growth factor (TGF) by several human tumor cell lines and their interactions with exogenously added epidermal growth factor (EGF) and insulin. TGF-like activities were present in all the conditioned media tested. The clonogenic capacity of the tumor cell lines had no correlation with the TGF-like activity production. EGF and insulin had a promoting colony-forming activity on tumor cells but this effect was not additive. Moreover, an inverse statistically significant correlation (-0.817, p less than 0.05) was found between the response to exogenous EGF and the EGF or TGF-alpha production by tumor cell lines. The EGF receptor (EGF-R) was not detected in any of the melanomas studied, nor in breast adenocarcinoma cell lines which were producers of EGF or TGF-alpha.
authors.affiliation: Unitat de Recerca Biomèdica, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Rueda
authors.forename: G
authors.initials: G
authors.lastname: Piñol
authors.forename: M
authors.initials: M
authors.lastname: Gonzalez-Garrigues
authors.forename: F
authors.initials: F
authors.lastname: Marti
authors.forename: A
authors.initials: A
authors.lastname: Fabra
mesh_terms: D002455:Cell Division; D002460:Cell Line; D004815:Epidermal Growth Factor / Q000494:pharmacology; D066246:ErbB Receptors / Q000032:analysis; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D015291:Transforming Growth Factors / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism*; D014410:Tumor Stem Cell Assay
chemical_list: D007328:Insulin; D004815:Epidermal Growth Factor; D015291:Transforming Growth Factors; D066246:ErbB Receptors