title: Stimulus-secretion coupling of arginine-induced insulin release. Metabolism of L-arginine and L-ornithine in pancreatic islets.
abstract: Exogenous L-arginine and L-ornithine rapidly accumulate in rat pancreatic islets. L-Arginine is converted to L-ornithine and urea. Endogenous or exogenous L-ornithine generates di- and polyamines, the putrescine turnover being faster than that of spermidine and spermine. However, the major pathway for L-ornithine metabolism consists of its transamination to L-glutamaldehyde and further conversion to L-glutamate. The amines and L-glutamate derived from exogenous L-ornithine are incorporated into islet proteins at the intervention of transglutaminase and cycloheximide-sensitive biosynthetic processes, respectively. These findings suggest the hypothesis that the insulinotropic action of L-arginine and L-ornithine could somehow be related to the metabolism of these cationic amino acids in islet cells.
authors.affiliation: Laboratory of Experimental Medicine, Brussels Free University, Belgium.
authors.forename: W J
authors.initials: WJ
authors.lastname: Malaisse
authors.forename: F
authors.initials: F
authors.lastname: Blachier
authors.forename: A
authors.initials: A
authors.lastname: Mourtada
authors.forename: J
authors.initials: J
authors.lastname: Camara
authors.forename: A
authors.initials: A
authors.lastname: Albor
authors.forename: I
authors.initials: I
authors.lastname: Valverde
authors.forename: A
authors.initials: A
authors.lastname: Sener
mesh_terms: D000818:Animals; D001119:Arginase / Q000378:metabolism; D001120:Arginine / Q000378:metabolism* / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D005971:Glutamates / Q000378:metabolism; D007328:Insulin / Q000378:metabolism*; D007515:Islets of Langerhans / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008954:Models, Biological; D009952:Ornithine / Q000031:analogs & derivatives / Q000378:metabolism* / Q000494:pharmacology; D010084:Oxidation-Reduction; D011073:Polyamines / Q000096:biosynthesis; D051381:Rats; D014508:Urea / Q000096:biosynthesis
chemical_list: D005971:Glutamates; D007328:Insulin; D011073:Polyamines; C025025:alpha-methylornithine; D014508:Urea; D001120:Arginine; D009952:Ornithine; D001119:Arginase; D005947:Glucose