6-Fluorocholesterol as a growth factor for the yeast mutant GL7.
6-Fluorocholesterol supports the growth of the sterol-requiring yeast mutant GL7 albeit less efficiently than cholesterol or ergosterol. When the fluoro analogue is combined with very much smaller amounts of cholesterol, the growth response to the sterol pair is synergistic, i.e., greater than additive. On further addition of trace amounts of ergosterol to the 6-fluorocholesterol-cholesterol pair, an additional synergistic growth response is observed. On 6-fluorocholesterol alone, the growth rate of the yeast mutant is slow initially, but after several transfers of such cells to the same media containing the fluoro analogue, growth improves substantially. When incorporated into artificial membranes, cholesterol and its 6-fluoro analogue have essentially identical effects on membrane fluidity as judged from microviscosity measurements. The contrasting responses of artificial membranes and whole cells to the 6-fluoro analogue of cholesterol might be due to sterol-protein interactions in natural membranes.
Zundel
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M
James Bryant Conant Laboratories, Department of Chemistry, Harvard University, Cambridge 02138.
Nambiar
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KP
Boswell
G
G
Bloch
K
K
D002784:Cholesterol / Q000031:analogs & derivatives* / Q000494:pharmacology; D004357:Drug Synergism; D004875:Ergosterol / Q000494:pharmacology; D008560:Membrane Fluidity / Q000187:drug effects; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D013261:Sterols / Q000302:isolation & purification; D014783:Viscosity
D013261:Sterols; C060869:6-fluorocholesterol; D002784:Cholesterol; D004875:Ergosterol