Synthesis of human endothelin-1 precursors in Escherichia coli.
Endothelin, the most potent vasoconstrictor found in nature, is thought to be important in the regulation of blood pressure and/or local blood distribution. Human placenta cDNA fragment encoding preproendothelin-1 (preproET-1) and its carboxyl terminal mature precursor (C-matured precursor) was expressed in E. coli. These products were characterized by both enzyme immunoassay and Western blot analysis.
Watanabe
T
T
Tsukuba Research Laboratories, Takeda Chemical Industries, Ltd, Ibaraki, Japan.
Itoh
Y
Y
Ogi
K
K
Kimura
C
C
Suzuki
N
N
Onda
H
H
D015153:Blotting, Western; D004247:DNA / Q000235:genetics*; D016232:Endothelins; D004730:Endothelium, Vascular; D004926:Escherichia coli / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D010452:Peptide Biosynthesis; D010455:Peptides / Q000235:genetics; D010957:Plasmids; D011498:Protein Precursors / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis*; D014158:Transcription, Genetic; D014169:Transformation, Bacterial
D016232:Endothelins; D010455:Peptides; D011498:Protein Precursors; D011994:Recombinant Proteins; D004247:DNA