[Cytokinins do not noticeably change the methylation of adenine residues of DNA in cultured Escherichia coli B].
6-Benzylaminopurine (6-BAP) (1 mg/ml) does not influence the growth of E. coli B cell cultures or the number of [8-14C] labeled N6-methyladenine (m6A) residues in the total DNA [(100.m6A/(A x m6A) = 1.7]. The growth of bacterial cells in the presence of adenine or cytokinins (6-BAP, kinetin, zeatin) (1 mg/ml) was unaccompanied by significant changes in the intracellular content of plasmid pBR 322. The mode of restriction by endonuclease Cfu I hydrolyzing the Gm6ATC site of plasmids pBR 322 from E. coli B cells grown in the presence of adenine or one of the above-mentioned cytokinins is identical. These plasmids also have identical restriction products Mbo I or Sau 3AI. Thus, the cytokinins under study do not markedly affect the methylation of adenine residues in total DNA of E. coli B cell cultures and the GATC sequence in plasmids pBR 322 isolated from these cells.
Vaniushin
B F
BF
Aleksandrushkina
N I
NI
Ogarkova
O A
OA
D000225:Adenine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D001593:Benzyl Compounds; D003583:Cytokinins / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000187:drug effects*; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D007701:Kinetin; D008745:Methylation; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010957:Plasmids; D011687:Purines
D000225:Adenine; D001593:Benzyl Compounds; D003583:Cytokinins; D004269:DNA, Bacterial; D007701:Kinetin; D010937:Plant Growth Regulators; D011687:Purines; C480551:benzylaminopurine; C000730567:6-methyladenine
Tsitokininy zametno ne izmeniaiut metilirovanie adeninovykh ostatkov DNK v kul'ture kletok Escherichia coli B.