title: [Tissue levels of locally applied cyclosporin A in the rabbit eye].
abstract: Cyclosporin A eye drops (4%) were applied to the conjunctival sac of pigmented rabbits for 4 and 6 weeks. Cyclosporin-A was eluted from biopsy specimens of treated eyes and measured by the commercially available radioimmunoassey. Therapeutic Cyclosporin A levels could be demonstrated with chronic application in the rabbit cornea 3 and 10 h after the last eye drop had been administered suggesting a pooling of the substance in corneal tissue. Other ocular compartments (conjunctiva, iris, lens, vitreous, and aqueous humor) gave results below the therapeutic levels. Immunohistological studies with indirect immunofluorescence demonstrated strong binding to corneal and conjunctival epithelial cell membranes.
authors.forename: L
authors.initials: L
authors.lastname: Stamer
authors.forename: M
authors.initials: M
authors.lastname: Böhnke
authors.forename: K
authors.initials: K
authors.lastname: Vogelberg
authors.forename: R
authors.initials: R
authors.lastname: Arndt
mesh_terms: D000818:Animals; D003524:Cyclosporins / Q000008:administration & dosage / Q000493:pharmacokinetics*; D005123:Eye / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D009883:Ophthalmic Solutions; D011817:Rabbits; D011863:Radioimmunoassay
chemical_list: D003524:Cyclosporins; D009883:Ophthalmic Solutions
vernacular_title: Gewebespiegel von lokal applizierten Ciclosporin-A (CS-A) am Kaninchenauge.