Effects of bradykinin, GTP gamma S, R59022 and N-ethylmaleimide on inositol phosphate production in NG108-15 cells.
Accumulation of inositol phosphates in NG108-15 neuroblastoma x glioma hybrid cells, pre-labeled for 24h to equilibrium, was stimulated by bradykinin, guanosine 5'-O-(3-thiotriphosphate) and the diacylglycerol kinase inhibitor R59022. Only the stimulation by bradykinin was inhibited by the bradykinin receptor antagonist [D-Arg0, Hyp3, Phe7, Thi5,8] bradykinin. Neither bradykinin nor R059022 increased the labeling of the inositol phospholipids. The sulfhydryl-alkylating reagent N-ethylmaleimide at 100 microM essentially abolished the stimulation caused by all three agents, possibly by preventing the binding of GTP to a guanine nucleotide-binding regulatory protein of as yet unknown size.
Chiang
C F
CF
Department of Psychiatry, Harvard Medical School, Boston, MA 02115.
Hauser
G
G
D000818:Animals; D001920:Bradykinin / Q000494:pharmacology*; D002460:Cell Line; D004347:Drug Interactions; D005033:Ethylmaleimide / Q000494:pharmacology*; D005910:Glioma; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate / Q000494:pharmacology; D006822:Hybrid Cells / Q000187:drug effects / Q000378:metabolism*; D007294:Inositol / Q000378:metabolism; D007295:Inositol Phosphates / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D009447:Neuroblastoma; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors; D011744:Pyrimidinones / Q000494:pharmacology*; D051381:Rats; D013844:Thiazoles / Q000494:pharmacology*; D013873:Thionucleotides / Q000494:pharmacology*
D007295:Inositol Phosphates; D010972:Platelet Activating Factor; D011744:Pyrimidinones; D013844:Thiazoles; D013873:Thionucleotides; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D007294:Inositol; D006160:Guanosine Triphosphate; C047505:R 59022; D005033:Ethylmaleimide; D001920:Bradykinin