title: Skin reactions to irritants assessed by non-invasive bioengineering methods.
abstract: Pathophysiological components of irritant contact dermatitis caused by 3 chemically-different irritants were investigated. 20 healthy volunteers were patch tested with sodium lauryl sulphate, nonanoic acid and hydrochloric acid on the flexor side of the upper arm. The skin response was evaluated after 24, 48 and 96 h by visual scoring and measured by the following bioengineering methods: transepidermal water loss measurement, electrical conductance for measurement of skin hydration, laser Doppler flowmetry for measurement of cutaneous blood flow and 20 MHz ultrasound A-scan for measurement of skin thickness. In spite of homogeneous inflammatory responses, significant differences in the severity of the injury to the skin barrier function caused by the different irritants were found. Also significant differences between irritants were found in the time course of development of maximum irritant reactions. Bioengineering methods indicating inflammatory responses (measurement of blood flow and skin thickness) were helpful in quantifying the irritant response in general, while bioengineering methods indicating epidermal damage (measurement of TEWL and electrical conductance) were helpful in classifying the individual irritants.
authors.affiliation: Dermatological Department H, Rigshospitalet, Copenhagen, Denmark.
authors.forename: T
authors.initials: T
authors.lastname: Agner
authors.forename: J
authors.initials: J
authors.lastname: Serup
mesh_terms: D000328:Adult; D001132:Arm; D003877:Dermatitis, Contact / Q000503:physiopathology*; D005227:Fatty Acids / Q000008:administration & dosage / Q000494:pharmacology; D005260:Female; D005712:Galvanic Skin Response / Q000187:drug effects; D006801:Humans; D006851:Hydrochloric Acid / Q000494:pharmacology; D007509:Irritants / Q000494:pharmacology*; D008297:Male; D010328:Patch Tests / Q000379:methods; D012727:Sex Characteristics; D012867:Skin / Q000033:anatomy & histology / Q000098:blood supply / Q000276:immunology*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013997:Time Factors; D014463:Ultrasonography / Q000379:methods; D014870:Water Loss, Insensible / Q000187:drug effects
chemical_list: D005227:Fatty Acids; D007509:Irritants; D012967:Sodium Dodecyl Sulfate; C008776:pelargonic acid; D006851:Hydrochloric Acid