Chromosome partition in Escherichia coli requires postreplication protein synthesis.
After inhibition of protein synthesis, the number of nuclear bodies (nucleoids) visible in cells of Escherichia coli B/rA corresponded closely to the number of completely replicated chromosomes. We calculated that nucleoid partition follows almost immediately after replication forks reach the chromosome terminus. We show that such a partition is dependent on protein synthesis and that this may reflect the requirement that cells must achieve a certain minimum length before partition (and subsequent cell division) can take place.
Donachie
W D
WD
Department of Molecular Biology, University of Edinburgh, Scotland.
Begg
K J
KJ
D001426:Bacterial Proteins / Q000096:biosynthesis*; D002876:Chromosomes, Bacterial / Q000502:physiology*; D004261:DNA Replication; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure; D013997:Time Factors
D001426:Bacterial Proteins