A cDNA clone encoding the precursor for a 10.2 kDa photosystem I polypeptide of barley.
Two cDNA clones for the barley photosystem I polypeptide which migrates with an apparent molecular mass of 9.5 kDa on SDS-polyacrylamide gels have been isolated using antibodies and an oligonucleotide probe. The determined N-terminal amino acid sequence for the mature polypeptide confirms the identification of the clones. The 644 base-pair sequence of one of the clones contains one large open reading frame coding for a 14,882 Da precursor polypeptide. The molecular mass of the mature polypeptide is 10 193 Da. The hydropathy plot of the polypeptide shows one membranespanning region with a predicted alpha-helix secondary structure. The gene for the 9.5 kDa polypeptide has been designated PsaH.
Okkels
J S
JS
Department of Plant Physiology, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark.
Scheller
H V
HV
Jepsen
L B
LB
Møller
B L
BL
D000595:Amino Acid Sequence; D001483:Base Sequence; D015153:Blotting, Western; D002734:Chlorophyll / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D001467:Hordeum / Q000235:genetics; D045342:Light-Harvesting Protein Complexes; D008969:Molecular Sequence Data; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D010940:Plant Proteins / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics*
D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D010940:Plant Proteins; D011498:Protein Precursors; D002734:Chlorophyll; D004247:DNA