title: The expression of enterotoxin A-B+ gene of V. cholerae in E. coli.
abstract: The cloning in E. coli of a cholerae toxin gene that is A-B+ has been successfully constructed by using DNA recombinant techniques. E. coli cells carrying the recombinant plasmid pMM-CTB have been shown to produce a large amount of CTB subunits which are secreted as extracellular proteins.
authors.affiliation: Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing.
authors.forename: Q J
authors.initials: QJ
authors.lastname: Ma
authors.forename: J G
authors.initials: JG
authors.lastname: Zhou
authors.forename: X Q
authors.initials: XQ
authors.lastname: Yu
authors.forename: C X
authors.initials: CX
authors.lastname: Liu
authors.forename: L S
authors.initials: LS
authors.lastname: Xiong
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Xu
authors.forename: C F
authors.initials: CF
authors.lastname: Huang
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D004274:DNA, Recombinant; D004768:Enterotoxins / Q000235:genetics* / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D011817:Rabbits; D011995:Recombination, Genetic; D014734:Vibrio cholerae / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D004274:DNA, Recombinant; D004768:Enterotoxins