Regional distribution of immunoreactive endothelin in rats.
By use of a specific radioimmunoassay for endothelin (ET), the regional distribution of ET-like immunoreactivity (LI) was studied in rats. The antiserum used cross-reacted equally with synthetic porcine and rat ET. Significant amounts of ET-LI are detectable not only in aorta, but also in kidney, lung, heart, liver and central nervous system. Gel chromatography of the tissue extracts revealed size heterogeneity of ET-LI; one major component eluting close to, but slightly larger than standard rat ET and the other minor component with a larger molecular weight. These data indicate that ET-LI is widely distributed throughout the various rat tissues, suggesting its possible involvement in a variety of organ functions.
Yoshimi
H
H
Hypertension-Endocrine Division, National Cardiovascular Center Research Institute and Hospital, Osaka, Japan.
Hirata
Y
Y
Fukuda
Y
Y
Kawano
Y
Y
Emori
T
T
Kuramochi
M
M
Omae
T
T
Marumo
F
F
D000818:Animals; D002850:Chromatography, Gel; D016232:Endothelins; D005260:Female; D007457:Iodine Radioisotopes; D010455:Peptides / Q000378:metabolism*; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013552:Swine; D014018:Tissue Distribution
D016232:Endothelins; D007457:Iodine Radioisotopes; D010455:Peptides