[UNG-dependent correction of molecular heteroduplexes of M13 phage DNA in Escherichia coli cells].
Correction of heteroduplex DNA obtained by hybridization of uracil-containing single-stranded M13mp18 phage DNA and "mutant" synthetic oligonucleotide with deletion of cytosine in SalGI site was studied in ung+ and ung- E. coli strains. Uracil-containing DNA was prepared after growth of phage in an E. coli strain dut- ung-. The DNA was hybridized with "mutant" oligonucleotide then complementary DNA chain was synthesized by T4 DNA polymerase. Ung+ and ung- E. coli cells were transformed by DNA. In all experiments mutation frequency in ung+ was higher than in ung- cells (approximately 6-fold) and reached 11-50%. Absolute number of mutants was higher in ung+ cells. The results indicate that high level of mutagenesis depends on uracil repair system polarizing the correction of heteroduplex DNA.
Golubovskaia
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VM
Aprelikova
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ON
Tomilin
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NV
D001483:Base Sequence; D003090:Coliphages / Q000235:genetics*; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D009692:Nucleic Acid Heteroduplexes; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000378:metabolism; D010766:Phosphorylation; D014170:Transformation, Genetic
D004279:DNA, Viral; D009692:Nucleic Acid Heteroduplexes; D009841:Oligonucleotides
UNG-zavisimaia korrektsiia molekuliarnykh geterodupleksov DNK faga M13 v kletkakh Escherichia coli.