Participation of the hup gene product in site-specific DNA inversion in Escherichia coli.
The closely related Escherichia coli genes hupA and hupB each encode a bacterial histone-like protein HU. We report here that DNA inversion mediated by hin, gin, pin and rci but not by cin is blocked in a hupA hupB double mutant, although inversions in these systems occur in the hupA or hupB single mutant as efficiently as in the wild-type strain. These findings show that HU protein participates in site-specific DNA inversion in E. coli and that only one subunit, either HU-1 or HU-2, is sufficient for this inversion.
Wada
M
M
Laboratory of Molecular Genetics, Riken, Tsukuba Life Science Center, Ibaraki, Japan.
Kutsukake
K
K
Komano
T
T
Imamoto
F
F
Kano
Y
Y
D001426:Bacterial Proteins / Q000235:genetics*; D007446:Chromosome Inversion; D004254:DNA Nucleotidyltransferases / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D009154:Mutation; D010957:Plasmids; D011995:Recombination, Genetic; D014170:Transformation, Genetic
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C040330:histone-like protein HU, bacteria; D004254:DNA Nucleotidyltransferases