Streptozotocin-induced diabetes reduces the density of [125I]-endothelin-binding sites in rat cardiac membranes.
The effect of acute, streptozotocin-induced diabetes on the affinity (KD), density (Bmax) and selectivity of specific, high affinity binding sites for [125I]-endothelin [( 125I]-ET) in rat cardiac membrane fragments was determined. Three days after a single i.v. bolus dose of streptozotocin (60 mg kg-1), the density of [125I]ET binding sites was reduced (P less than 0.01) without changes in affinity or selectivity.
Nayler
W G
WG
Department of Medicine, University of Melbourne, Austin Hospital, Heidelberg, Victoria, Australia.
Liu
J J
JJ
Panagiotopoulos
S
S
Casley
D J
DJ
D000818:Animals; D001665:Binding Sites; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D016232:Endothelins; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D008297:Male; D008566:Membranes / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D016232:Endothelins; D007457:Iodine Radioisotopes; D010455:Peptides