Contrasts in antigen expression in the erythrocytic and exoerythrocytic stages of rodent malaria.
The time and site of expression of five antigens, recognized by monoclonal antibodies raised against blood-stage parasites, were studied in the exoerythrocytic stage of Plasmodium berghei using indirect immunofluorescent antibody staining. Two monoclonal antibodies (W 3.5, I 2.6), which stain the cytoplasm of infected erythrocytes, did not stain the cytoplasm of the infected liver cell but stained the parasite itself suggesting a difference in the antigenic architecture of the erythrocytic and exoerythrocytic parasites. Another antibody (17.6.1) revealed a further difference in the antigenic composition of the blood and liver-stage parasites being expressed almost exclusively in the former. Two others (C139 and 17.3.9) showed broadly similar patterns of expression in these two stages of the malarial life-cycle.
Suhrbier
A
A
Department of Pure and Applied Biology, Imperial College, London.
Wiser
M F
MF
Winger
L
L
Harte
P
P
Newton
M F
MF
Hodivala
K J
KJ
Nicholas
J
J
Sinden
R E
RE
D000818:Animals; D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan / Q000096:biosynthesis*; D002460:Cell Line; D004912:Erythrocytes / Q000469:parasitology*; D005455:Fluorescent Antibody Technique; D008099:Liver / Q000469:parasitology*; D051379:Mice; D010962:Plasmodium berghei / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan