[Evaluation of phagocytosis of alpha hemolytic strains of Escherichia coli using the fluorescence method].
The authors investigated phagocytosis in 37 alpha haemolytic strains of E. coli by the fluorescent method, after opsonization of bacteria with 5% and concentrated human serum. The strains were isolated from infants with gastrointestinal and extraintestinal infections and belonged to eleven serogroups. During opsonization of the strain with concentrated serum, as compared with opsonization of strains with 5% serum, higher rates of killing were recorded. As compared with a control strain of E. coli K12 after opsonization with concentrated serum in the examined strains the value of killing was on average by 20% lower. Differences in the killing of examined strains were recorded also within a single serogroup which may be ascribed to the different provision of strains with plasmids.
Siegfried
L
L
Puzová
H
H
Hlaucová
J
J
Durovicová
J
J
D001426:Bacterial Proteins / Q000096:biosynthesis*; D004926:Escherichia coli / Q000378:metabolism; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins / Q000096:biosynthesis*; D006801:Humans; D009504:Neutrophils / Q000502:physiology*; D009895:Opsonin Proteins; D010587:Phagocytosis
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli; D009895:Opsonin Proteins
Hodnotenie fagocytózy alfa hemolytických kmenov Escherichia coli fluorescencnou metódou.