Isolation of cardiac ventricular myocytes from newborn rats by use of fractional elutriation centrifugation.
Isolation of cardiac ventricular myocytes from newborn rats (0-4 days old) by use of elutriation centrifugation is described. By the use of fractional centrifugation, a homogeneous myocyte population with high purity was obtained without any further procedures, yielding about 10(6) myocytes/rat. When 48-hr monolayer cultures were established, the cells showed normal pulsatory contractions. A morphological evaluation of such cultures is given.
Diamant
B
B
Department of Pharmacology, University of Copenhagen, Denmark.
Steiness
E
E
Hansen
B F
BF
Jensen
M B
MB
D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D002478:Cells, Cultured; D002498:Centrifugation; D003584:Cytological Techniques; D009124:Muscle Proteins / Q000378:metabolism; D009206:Myocardium / Q000166:cytology*; D010316:Particle Size; D051381:Rats; D011919:Rats, Inbred Strains
D009124:Muscle Proteins