Replacement of potassium ions by ammonium ions in different micro-organisms grown in potassium-limited chemostat culture.
The biomass concentration extant in potassium-limited cultures of either Klebsiella pneumoniae or Bacillus stearothermophilus (when growing at a fixed temperature and dilution rate in a glucose/ammonium salts medium) increased progressively as the medium pH value was raised step-wise from 7.0 to 8.5. Because the macromolecular composition of the organisms did not vary significantly, this increase in biomass could not be attributed to an accumulation of storage-type polymers but appeared to reflect a pH-dependent decrease in the cells' minimum K+ requirement. Significantly, this effect of pH was not evident with cultures in which no ammonium salts were present and in which either glutamate or nitrate was added as the sole nitrogen source; however, it was again manifest when various concentrations of NH4Cl were added to the glutamate-containing medium. This suggested a functional replacement of K+ by NH4+, a proposition consistent with the close similarity of the ionic radii of the potassium ion (1.33 A) and the ammonium ion (1.43 A). At pH 8.0, and with a medium containing both glutamate (30 mM) and NH4Cl (100 mM), cultures of B. stearothermophilus would grow without added potassium at a maximum rate of 0.7 h-1. Under these conditions the cells contained maximally 0.1% (w/w) potassium (derived from contaminating amounts of this element in the medium constituents), a value which should be compared with one of 1.4% (w/w) for cells growing in a potassium-limited medium containing initially 0.5 mM K+.(ABSTRACT TRUNCATED AT 250 WORDS)
Buurman
E T
ET
Department of Microbiology, University of Amsterdam, The Netherlands.
Pennock
J
J
Tempest
D W
DW
Teixeira de Mattos
M J
MJ
Neijssel
O M
OM
D003470:Culture Media; D001411:Geobacillus stearothermophilus / Q000254:growth & development*; D006863:Hydrogen-Ion Concentration; D007711:Klebsiella pneumoniae / Q000254:growth & development*; D011188:Potassium / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds / Q000378:metabolism*
D003470:Culture Media; D000644:Quaternary Ammonium Compounds; D011188:Potassium