[Use of nonradioactive probes to detect the gene for 3-aminoside-acetyltransferase type IV in strains of Escherichia coli and Salmonella spp].
Forty-three isolates of Escherichia coli, Salmonella dublin and S. thyphimurium of animal origin were studied by colony hybridization using an intragenic probe specific for the gene encoding 3-aminoglycoside acetyltransferase type IV, which confers resistance to apramycin and gentamicin. Non-radioactive biotinylated and sulphonated DNA probes were used. No false-positive results were found and the positive results obtained were in agreement with those previously revealed with radioactive probes.
Perrin
B
B
Ministère de l'Agriculture, Direction générale de l'Alimentation, Lyon, France.
Solsona
M
M
Chaslus-Dancla
E
E
Martel
J L
JL
D000123:Acetyltransferases / Q000235:genetics*; D015342:DNA Probes; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012475:Salmonella / Q000235:genetics*
D015342:DNA Probes; D000123:Acetyltransferases; C019681:aminoglycoside acetyltransferase
Utilisation de sondes non radioactives pour la détection du gène de la 3-aminoside-acétyltransférase type IV chez des souches de Escherichia coli et Salmonella spp.