Transcription of the 5 S rRNA gene of Saccharomyces cerevisiae requires a promoter element at +1 and a 14-base pair internal control region.
In vitro transcription of templates containing deletion-substitution mutations has localized two essential promoter elements of the 5 S rRNA gene of Saccharomyces cerevisiae. A promoter element spanning the start site of transcription extends from -14 to +8, and a short internal control region (ICR) extends from +81 to +94. Changes in spacing between these elements by more than a few base pairs significantly reduce transcription. The site of RNA polymerase III transcription factor A (TFIIIA) binding, mapped by determination of the G residues that are protected from methylation on exposure of the TFIIIA.5 S DNA complex to dimethyl sulfate, is coincident with the ICR. Incorporation of TFIIIC into the TFIIIA.5 S rRNA gene complex protects additional G residues 5' and 3' of the ICR from methylation.
Challice
J M
JM
Department of Biochemistry, University of Toronto, Ontario, Canada.
Segall
J
J
D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D004275:DNA, Ribosomal / Q000235:genetics*; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009154:Mutation; D011401:Promoter Regions, Genetic; D012335:RNA, Ribosomal / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013698:Templates, Genetic; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D014981:Xenopus
D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; D012341:RNA, Ribosomal, 5S; D014157:Transcription Factors