title: Unexpected loss of immunoreactivity of a glucose oxidase-labeled antigen in a steroid enzyme immunoassay system.
abstract: Glucose oxidase-labeled 11-deoxycortisol was tested for immunoreactivity in an enzyme immunoassay system using a monoclonal antibody. The antigen was labeled by the N-succinimidyl ester method. It was found that the binding affinity of the label to the anti-steroid antibody markedly decreased during storage; the half-life time was ca. 4.5 d.
authors.forename: H
authors.initials: H
authors.lastname: Hosoda
authors.forename: M
authors.initials: M
authors.lastname: Itoh
authors.forename: R
authors.initials: R
authors.lastname: Tsukamoto
authors.forename: T
authors.initials: T
authors.lastname: Nambara
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology*; D005949:Glucose Oxidase / Q000276:immunology*; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D013256:Steroids / Q000276:immunology*
chemical_list: D000941:Antigens; D013256:Steroids; D005949:Glucose Oxidase