title: Studies on bilokinase, a biliary plasminogen activator: immunologic property and organ distribution.
abstract: The aim of the present study was to elucidate the immunologic difference between bilokinase, a plasminogen activator in bile, and urokinase, an urinary plasminogen activator, and to demonstrate the organ distribution of these antigens. Polyclonal antibodies against the highly purified activators (bovine-bilokinase, -urokinase and human urokinase) were raised in rabbits or guinea pigs. Immunologic precipitin reactions and quenching were found to occur only between the activators and their respective antisera. Histo-immunologic demonstration of the activators revealed that bilokinase-antigen was localized mainly in the hepatocytes as well as in the mucosa of the gall bladder, whereas urokinase-antigen was present in the epithelium of the urinary tubules. The liver may have a potential role in the hepatobiliary fibrinolytic system.
authors.affiliation: College of Agriculture and Veterinary Medicine, Nihon University, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ariga
authors.forename: S
authors.initials: S
authors.lastname: Oshiba
authors.forename: T
authors.initials: T
authors.lastname: Seki
authors.forename: H
authors.initials: H
authors.lastname: Imai
authors.forename: S
authors.initials: S
authors.lastname: Imai
authors.forename: H
authors.initials: H
authors.lastname: Sawai
mesh_terms: D000818:Animals; D002417:Cattle; D010450:Endopeptidases / Q000032:analysis / Q000276:immunology* / Q000502:physiology; D006168:Guinea Pigs; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D011233:Precipitin Tests; D011817:Rabbits; D014018:Tissue Distribution; D014568:Urokinase-Type Plasminogen Activator / Q000276:immunology
chemical_list: D007106:Immune Sera; D010450:Endopeptidases; D014568:Urokinase-Type Plasminogen Activator; C019790:bilokinase