Comparative enzymatic study of HPLC-fractionated Crotalus venoms.
1. Ten venoms of the genus Crotalus (Crotalus adamanteus, Crotalus atrox, Crotalus durissus durissus, Crotalus horridus horridus, Crotalus lepidus, Crotalus polystictus, Crotalus molossus molossus, Crotalus pusillus, Crotalus scutulatus scutulatus, venom B, and Crotalus viridis lutosus) were fractionated using HPLC anion and cation exchange chromatography. 2. HPLC venom fractions were tested for hemorrhagic, hemolytic, and proteolytic activities. 3. Crude Virginia opossum (Didelphis virginiana) serum neutralized the hemorrhagic activity of HPLC fractions.
Soto
J G
JG
Department of Biology, Texas A&I University, Kingsville 78363.
Perez
J C
JC
Lopez
M M
MM
Martinez
M
M
Quintanilla-Hernandez
T B
TB
Santa-Hernandez
M S
MS
Turner
K
K
Glenn
J L
JL
Straight
R C
RC
Minton
S A
SA
D000818:Animals; D000997:Antivenins / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003435:Crotalid Venoms / Q000032:analysis* / Q000378:metabolism; D006461:Hemolysis; D006470:Hemorrhage / Q000139:chemically induced; D009893:Opossums; D010447:Peptide Hydrolases / Q000378:metabolism
D000997:Antivenins; D003435:Crotalid Venoms; D010447:Peptide Hydrolases