Investigation of the structure of the capsular polysaccharide of Escherichia coli K55 using Klebsiella bacteriophage phi 5.
The structure of the capsular polysaccharide from Escherichia coli O9:K55 (N 24c) has been studied, using methylation analysis, degradation by bacteriophage, and n.m.r. spectroscopy. Depolymerisation of the K55 polysaccharide, using the lyase enzyme borne by Klebsiella phi 5, yielded a tri- and a hexa-saccharide, analysis of which indicated the following repeating unit. (formula; see text) This structure differs from that for the repeating unit of the capsular polysaccharide of Klebsiella K5 only in the position of acetylation (position 2 of the glucose residue).
Anderson
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School of Pharmaceutical Sciences, Rhodes University, Grahamstown, South Africa.
Parolis
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D001435:Bacteriophages / Q000378:metabolism; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000276:immunology*; D007709:Klebsiella / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification
D011135:Polysaccharides, Bacterial