title: Expression of nuclear lamins in mammalian somatic cells lacking cytoplasmic intermediate filament proteins.
abstract: Using immunofluorescence and immunoblotting techniques, we have examined the composition of the nuclear lamina in murine plasmacytoma cells, MPC-11, exposed to the phorbol ester TPA as well as in two cell lines devoid of cytoplasmic intermediate filament proteins, the human adrenal cortex carcinoma-derived cells SW-13 and the clone C6-M-D4 derived from the rat glial cell line C6. Our results show that the inhibition of proliferation and the induction of vimentin synthesis observed in TPA-treated MPC-11 populations are not paralleled by changes in the lamin complement of these cells, which contain lamin B but lack lamins A and C. Furthermore, the analysis performed on SW-13 and C6-M-D4 cell lines clearly demonstrates that mammalian somatic cells display considerable variations in lamin expression and indicates that lamin B may be the only lamin species constitutively expressed in mammalian cells.
authors.affiliation: Max-Planck-Institut für Zellbiologie, Ladenburg/Heidelberg, Federal Republic of Germany.
authors.forename: M
authors.initials: M
authors.lastname: Paulin-Levasseur
authors.forename: A
authors.initials: A
authors.lastname: Scherbarth
authors.forename: G
authors.initials: G
authors.lastname: Giese
authors.forename: K
authors.initials: K
authors.lastname: Röser
authors.forename: W
authors.initials: W
authors.lastname: Bohn
authors.forename: P
authors.initials: P
authors.lastname: Traub
mesh_terms: D000306:Adrenal Cortex Neoplasms; D000818:Animals; D002467:Cell Nucleus / Q000032:analysis*; D003593:Cytoplasm; D005455:Fluorescent Antibody Technique; D006801:Humans; D015151:Immunoblotting; D007381:Intermediate Filament Proteins; D034921:Lamin Type B; D034882:Lamins; D051379:Mice; D009687:Nuclear Proteins / Q000032:analysis*; D010954:Plasmacytoma; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D007381:Intermediate Filament Proteins; D034921:Lamin Type B; D034882:Lamins; D009687:Nuclear Proteins; D013755:Tetradecanoylphorbol Acetate