Serum can act as a shear protecting agent in agitated hybridoma cell cultures.
Hybridoma cells, S3H5/gamma 2bA2, were grown in spinner flasks containing RPMI 1640 media at different levels of serum. Cells in low serum media (1% fetal bovine serum) were more sensitive to shear induced by mechanical agitation than cells in high serum media (10%), indicating that serum can protect cells from shear induced by mechanical agitation. The data suggest that serum alters the physiological properties of the cells to make them more shear-resistant, rather than by changing the physico-chemical properties of medium.
Lee
G M
GM
Department of Chemical Engineering, University of Michigan, Ann Arbor.
Savinell
J M
JM
Palsson
B O
BO
D000818:Animals; D002455:Cell Division; D003470:Culture Media; D003584:Cytological Techniques; D006825:Hybridomas / Q000166:cytology*; D013314:Stress, Mechanical
D003470:Culture Media