title: Abnormal centrosomes in cold-treated Drosophila embryos.
abstract: In this study we examine the effect on the centrosomes of cold treatment of early Drosophila embryos. Prolonged cold treatment during the mitotic divisions which lead to the formation of the blastoderm causes arrest at metaphase of the nuclear divisions. When examined with immunofluorescence microscopy the mitotic spindles show marked pole splitting with the formation of supernumerary and irregularly sized centers, all able to nucleate microtubules. In embryos recovered for longer periods the additional organizing centers become ring-shaped and lose their nucleating properties. Cold treatment of embryos during the cellularization of the blastoderm results in marked fragmentation of the centrosomes, but nucleating capacity is preserved. Sometimes the centrioles come away from the pericentriolar material and their structure is seen to be modified.
authors.affiliation: Department of Evolutionary Biology, University of Siena, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Callaini
authors.forename: D
authors.initials: D
authors.lastname: Marchini
mesh_terms: D000818:Animals; D002453:Cell Cycle; D002502:Centrioles / Q000648:ultrastructure*; D003080:Cold Temperature; D003593:Cytoplasm / Q000648:ultrastructure; D004330:Drosophila / Q000196:embryology*; D005455:Fluorescent Antibody Technique; D008854:Microscopy, Electron; D008870:Microtubules / Q000648:ultrastructure