[Use of an inhibition method of ELISA in the serologic diagnosis of dengue. Preliminary report].
This paper reports the standardization of an ELISA (enzyme-linked immunosorbent assay) inhibition method for the serologic diagnosis of dengue, with the use of non-purified antigen. The conditions and criteria of the test for detection of antibodies for a group of 82 sera (12 monosera and 35 paired sera) are established. The results are compared with those obtained by hemagglutination inhibition and a good agreement between the two tests, as well as greater sensitivity of ELISA, is found. Some advantages of this technique in connection with hemagglutination inhibition are listed; they are: easy performance, rapidity, no need for serum treatment, and the use of viral suspensions without using any method of extraction.
Vázquez Ramudo
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Fernández Llanes
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D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D003715:Dengue / Q000175:diagnosis* / Q000276:immunology; D003716:Dengue Virus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D051379:Mice; D011237:Predictive Value of Tests
D000914:Antibodies, Viral; D007106:Immune Sera
Utilización de un método de inhibición de ELISA en el diagnóstico serológico del dengue. Reporte preliminar.