Elimination of the nucleolus from the nucleus of a living cell by centrifugation (a literature review).
The following effects involving the nucleolus take place during centrifugation of living cells at centrifugal forces of several thousand g to several hundred thousand g: settling of the nucleolus in centrifugal direction on the nuclear envelope; pulling the latter as a long stalk with the nucleolus at its end (or alternatively an easy perforation of the nuclear envelope by the nucleolus); release of the nucleolus into the cytoplasm or its expulsion out of the cell; occasional stratification of the nucleolus in the nucleus; fusion of many nucleoli together under centrifugal pressure. The asymmetric topography of the nuclear envelope is considered to be one of the causes of its different resistance to the penetration of the nucleolus. Elimination of the nucleolus from cancer cell nuclei to test the nucleolar contribution to cell malignancy is suggested as one conceivable application of the centrifugal technique of cell enucleolation.
Moroz
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D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D002466:Cell Nucleolus; D002467:Cell Nucleus; D002498:Centrifugation; D010944:Plants