Characteristics and detection principles of a new enzyme label producing a long-term chemiluminescent signal.
A new enzyme label system is described which is superior to all existing chemiluminescence labels used in immunoassays. The system consists of the enzyme xanthine oxidase with hypoxanthine as substrate. The signal reagent contains perborate, an Fe-EDTA complex and luminol. The enzyme preparation and the signal reagent are very stable upon storage. The main features of the system are a long duration of the chemiluminescent signal (half-life time of 30 hours) and a very low limit of detection (about 3 amol). Possibilities and implications for the use of various measuring systems are discussed.
Jansen
E H
EH
National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
van den Berg
R H
RH
Zomer
G
G
D006207:Half-Life; D019271:Hypoxanthine; D007042:Hypoxanthines; D007124:Immunoenzyme Techniques; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D014969:Xanthine Oxidase
D007042:Hypoxanthines; D007202:Indicators and Reagents; D019271:Hypoxanthine; D014969:Xanthine Oxidase