Immunohistochemical localization of relaxin in human prostate.
We identified relaxin in human male prostate by use of an anti-human relaxin analogue polyclonal antibody and the avidin-biotin-immunoperoxidase method. The antibody was obtained by immunizing a rabbit with a synthetic human relaxin analogue which has 95% sequence homology with native human relaxin. Human prostate tissues incubated with the anti-human relaxin analogue exhibited positive immunostaining up to an antibody dilution of 1:3200. Inhibition of immunostaining with this antibody by excess relaxin analogue demonstrated specificity of the antibody. The exact role of relaxin in human male reproductive physiology remains to be fully elucidated.
Sokol
R Z
RZ
Harbor-UCLA Medical Center, UCLA School of Medicine, Torrance 90502.
Wang
X S
XS
Lechago
J
J
Johnston
P D
PD
Swerdloff
R S
RS
D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D011467:Prostate / Q000032:analysis*; D012065:Relaxin / Q000032:analysis*
D012065:Relaxin