title: Characterization of recombinant thioesterase and acyl carrier protein domains of chicken fatty acid synthase expressed in Escherichia coli.
abstract: Fatty acid synthase of animal tissue is a multifunctional enzyme comprised of two identical subunits, each containing seven partial activities and a site for the prosthetic group, 4'-phosphopantetheine (acyl carrier protein). We have recently isolated cDNA clones of chicken fatty acid synthase coding for the dehydratase, enoyl reductase, beta-ketoacyl reductase, acyl carrier protein, and thioesterase domains (Chirala, S.S., Kasturi, R., Pazirandeh, M., Stolow, D.T., Huang, W.Y., and Wakil, S.J. (1989) J. Biol. Chem. 264, 3750-3757). To gain insight into the structure and function of the various domains, the portion of the cDNA coding for the acyl carrier protein and thioesterase domains was expressed in Escherichia coli by using an expression vector that utilizes the phage lambda PL promoter. The recombinant protein was efficiently expressed and purified to near homogeneity using anion-exchange and hydroxyapatite chromatography. As expected from the coding capacity of the cDNA expressed, the protein has a molecular weight of 43,000 and reacts with antithioesterase antibodies. The recombinant thioesterase was found to be enzymatically active and has the same substrate specificity and kinetic properties as the native enzyme of the multifunctional synthase. Treatment of the recombinant protein with alpha-chymotrypsin results in the cleavage of the acyl carrier protein and thioesterase domain junction sequence at exactly the same site as with native fatty acid synthase. The amino acid composition of the purified recombinant protein revealed the presence of 0.6 mol of beta-alanine/mol of protein, indicating partial pantothenylation of the recombinant acyl carrier protein domain. These results indicate that the expressed protein has a conformation similar to the native enzyme and that its folding into functionally active domains is independent of the remaining domains of the multifunctional synthase subunit. These conclusions are consistent with the proposal that the multifunctional synthase gene has evolved from fusion of component genes.
authors.affiliation: Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030.
authors.forename: M
authors.initials: M
authors.lastname: Pazirandeh
authors.forename: S S
authors.initials: SS
authors.lastname: Chirala
authors.forename: W Y
authors.initials: WY
authors.lastname: Huang
authors.forename: S J
authors.initials: SJ
authors.lastname: Wakil
mesh_terms: D000213:Acyl Carrier Protein / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D002645:Chickens; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D064429:Fatty Acid Synthases / Q000235:genetics / Q000378:metabolism*; D005822:Genetic Vectors; D007700:Kinetics; D008970:Molecular Weight; D010205:Pantothenic Acid / Q000378:metabolism; D010446:Peptide Fragments / Q000302:isolation & purification; D010957:Plasmids; D011485:Protein Binding; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D013869:Thiolester Hydrolases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000213:Acyl Carrier Protein; D010446:Peptide Fragments; D011994:Recombinant Proteins; D010205:Pantothenic Acid; D004247:DNA; D064429:Fatty Acid Synthases; D013869:Thiolester Hydrolases