title: Genetic requirements for hyper-recombination by very short patch mismatch repair: involvement of Escherichia coli DNA polymerase I.
abstract: It has been established that very short patch (VSP) mismatch repair, depending in Escherichia coli on MutL, MutS and Dcm functions, is responsible for the hyper-recombinogenic effect of a class of genetic markers. We show that VSP repair requires the presence of the complete DNA polymerase I enzyme. The absence of endonuclease activities involved in the repair of base-loss sites, Nth, Nfo and Xth, does not affect VSP repair. Implications for the mechanism of the VSP repair are discussed.
authors.affiliation: Institut Jacques Monod, Paris, France.
authors.forename: S
authors.initials: S
authors.lastname: Dzidic
authors.forename: M
authors.initials: M
authors.lastname: Radman
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D003433:Crosses, Genetic; D004256:DNA Polymerase I / Q000378:metabolism*; D004260:DNA Repair; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005819:Genetic Markers; D009154:Mutation; D011995:Recombination, Genetic
chemical_list: D005819:Genetic Markers; D004256:DNA Polymerase I