Immunohistochemical detection of prolactin and its receptors in human testis.
Prolactin (PRL) and PRL receptors were localized by light microscopic immunohistochemistry in the human testes on interstitial Leydig-like cells. Similar stainings were obtained with both monoclonal antihuman PRL antibodies and polyclonal antibodies raised against rabbit mammary gland PRL receptors. Moreover, the detection of PRL binding sites by radio-receptor assay on the same samples gave a confirmation of these results. This suggests that PRL might act directly on Leydig cells in the man, in the same way as in the rodent.
Bouhdiba
M
M
Laboratoire d'Histologie-Embryologie, Josas, France.
Leroy-Martin
B
B
Peyrat
J P
JP
Saint Pol
P
P
Djiane
J
J
Leonardelli
J
J
D000368:Aged; D000911:Antibodies, Monoclonal; D002277:Carcinoma / Q000209:etiology; D005404:Fixatives; D006801:Humans; D007124:Immunoenzyme Techniques; D007985:Leydig Cells; D008297:Male; D008875:Middle Aged; D011388:Prolactin / Q000032:analysis / Q000276:immunology; D011471:Prostatic Neoplasms / Q000209:etiology; D011869:Radioligand Assay; D011981:Receptors, Prolactin / Q000032:analysis* / Q000276:immunology; D013737:Testis / Q000032:analysis*
D000911:Antibodies, Monoclonal; D005404:Fixatives; D011981:Receptors, Prolactin; D011388:Prolactin