Immunohistologic demonstration of type II collagen in synovial fluid phagocytes of osteoarthritis and rheumatoid arthritis patients.
We were able to demonstrate type II collagen in synovial phagocytes of osteoarthritis (OA) and rheumatoid arthritis (RA) patients, using a monoclonal antibody to human type II collagen and immunoperoxidase staining. In addition, using immunoelectron microscopy, we demonstrated labeled fragments in synovial phagocytes of both RA and OA patients. This immunohistochemical assay may prove to be a sensitive indicator of cartilage erosion in patients with OA and RA.
Moreland
L W
LW
Division of Clinical Immunology and Rheumatology, University of Alabama, Birmingham 35294.
Stewart
T
T
Gay
R E
RE
Huang
G Q
GQ
McGee
N
N
Gay
S
S
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D001799:Blood Sedimentation; D003094:Collagen / Q000032:analysis*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D010003:Osteoarthritis / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D010586:Phagocytes / Q000276:immunology*; D013582:Synovial Fluid / Q000166:cytology*
D000911:Antibodies, Monoclonal; D003094:Collagen