title: Interaction of turkey complement with Escherichia coli isolated from turkeys.
abstract: The role of turkey complement in a serum bactericidal reaction was determined using serum-sensitive and serum-resistant Escherichia coli isolated from turkeys. Inactivation of complement by heating serum (56 C for 40 minutes) or by treating serum with 10 mM EDTA eliminated bactericidal activity. Serum-sensitive E coli organisms were killed by turkey serum treated with 10 mM ethylene glycol-bis-beta-(aminoethyl ether)-N,N,N',N'-tetraacetic acid and 5 mM MgCl2. Exposure of normal turkey serum to serum-sensitive or serum-resistant E coli resulted in equivalent reductions in hemolytic activity of serum. Treatment of serum-resistant E coli with antibody rendered the bacteria sensitive to bactericidal effects of normal turkey serum. Serum-sensitive E coli organisms were readily killed by an alternative complement pathway, serum-sensitive and serum-resistant E coli activated the complement system equally well, and antibody was required for complement-mediated killing of certain serum-resistant E coli organisms from turkeys.
authors.affiliation: Department of Animal Science, Iowa State University, Ames 50011.
authors.forename: M G
authors.initials: MG
authors.lastname: Ellis
authors.forename: L H
authors.initials: LH
authors.lastname: Arp
authors.forename: S J
authors.initials: SJ
authors.lastname: Lamont
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001770:Blood Bactericidal Activity; D003165:Complement System Proteins / Q000276:immunology*; D004926:Escherichia coli / Q000276:immunology* / Q000302:isolation & purification; D014422:Turkeys / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D003165:Complement System Proteins