title: Influence of the route of glucose administration on hepatic glycogen repletion.
abstract: To delineate the role of the route of glucose administration on liver glycogen synthesis, we administered [1-13C]glucose either by intravenous or intraduodenal infusion to chronically catheterized 24-h fasted rats and performed 1) intravenous-hyperglycemic clamp (group I, n = 9), portal vein plasma glucose and insulin concentrations were 216 +/- 6 mg/dl and 23.9 +/- 4.2 ng/ml; 2) intraduodenal-hyperglycemic infusion (group II, n = 8), portal vein glucose and insulin concentrations were 219 +/- 6 mg/dl and 17.5 +/- 2.7 ng/ml; and 3) intravenous-hyperglycemic-suprahyperinsulinemic clamp (group III, n = 5), portal vein glucose and insulin concentrations were 203 +/- 12 mg/dl and 44.6 +/- 5.0 ng/ml. The mean glucose infusion rates (mumol.kg-1.min-1) and glycogenic rates (mumol.g liver-1.min-1) were 201 +/- 8, 0.34 +/- 0.05; 129 +/- 3, 0.73 +/- 0.11; and 269 +/- 19, 0.38 +/- 0.08 in groups I-III, respectively. The percent of glycogen synthesized by the direct pathway was group I = 43 +/- 6%, group II = 44 +/- 6%, and group III = 46 +/- 7%. In conclusion, despite similar or lower portal vein insulin and glucose concentrations, the intraduodenal route of glucose administration (group II), compared with the intravenous route (groups I and III), markedly increased the total amount of liver glycogen synthesized without altering the percent of the direct vs. indirect pathways by which liver glycogen was repleted.
authors.affiliation: Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06510.
authors.forename: G I
authors.initials: GI
authors.lastname: Shulman
authors.forename: L
authors.initials: L
authors.lastname: Rossetti
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D004386:Duodenum; D005947:Glucose / Q000008:administration & dosage* / Q000494:pharmacology; D006003:Glycogen / Q000378:metabolism*; D006706:Homeostasis; D007262:Infusions, Intravenous; D007267:Injections; D007328:Insulin / Q000097:blood; D008099:Liver / Q000378:metabolism*; D008297:Male; D009994:Osmolar Concentration; D011169:Portal Vein; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014680:Veins
chemical_list: D001786:Blood Glucose; D007328:Insulin; D006003:Glycogen; D005947:Glucose