Two subunits of mannose permease, II-PMan and II-MMan, of Escherichia coli mediate coliphage N4 infection.
Mutant strains of Escherichia coli that lack one or all of the intact components of mannose permease do not support the growth of phage N4. Complementation experiments using three recombinant plasmids containing DNA fragments coding for the subunits of mannose permease revealed that among the three component subunits, II-PMan and II-MMan alone are sufficient to confer N4 sensitivity.
Kim
K
K
Department of Biological Science and Engineering, Korea Advanced Institute of Science and Technology, Seoul.
Yoo
O J
OJ
D003090:Coliphages / Q000235:genetics* / Q000472:pathogenicity; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005815:Genetic Code; D005816:Genetic Complementation Test; D009154:Mutation; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D011994:Recombinant Proteins / Q000378:metabolism; D013489:Suppression, Genetic
D004274:DNA, Recombinant; D011994:Recombinant Proteins; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C037992:phosphoenolpyruvate-mannose phosphotransferase