[Detection of glycosphingolipids of ABH and Le antigens on thin-layer plates using the PAP method].
Stroma from hemolyzed erythrocytes of blood groups 0, A1, B and A1B were obtained and subjected to butanol/phosphate buffer extraction. This extract was separated using HPTLC, and the ABH and Le substances were detected on the chromatogram using the PAP technique. The staining of the bands allowed specific demonstration of the serologically active glycosphingolipids present in the ABH and Le blood group substances. The antigens of the AB0 system showed a 3- to 12-band pattern. Each of the antigens Lea and Leb presented 3 bands. The slight differences in the levels of glycosphingolipids of equal chain lengths are probably due to differences in their chemical structures.
Hauser
R
R
Institut für Rechtsmedizin der Medizinischen Akademie Gdansk, Polen.
Rand
S
S
Lenz
J
J
Brinkmann
B
B
D000017:ABO Blood-Group System / Q000235:genetics*; D001788:Blood Grouping and Crossmatching / Q000379:methods; D002855:Chromatography, Thin Layer; D006028:Glycosphingolipids / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D007519:Isoantigens / Q000032:analysis; D007983:Lewis Blood Group Antigens / Q000235:genetics*
D000017:ABO Blood-Group System; D006028:Glycosphingolipids; D007519:Isoantigens; D007983:Lewis Blood Group Antigens
Nachweis von Glykosphingolipiden der ABH- und Le-Antigene auf Dünnschichtplatten mittels der PAP-Methode.