Isolation of a potential DNA probe for Toxoplasma gondii.
A genomic DNA library for Toxoplasma gondii has been constructed in plasmid pUC8. Some recombinant plasmids (pJMBG1-pJMBG55) were analysed by restriction endonuclease mapping and by hybridization to DNA isolated from T. gondii and from human tissue culture cells. A number of recombinant plasmids, or DNA fragments derived from these, hybridizing only to Toxoplasma DNA have been identified. These are currently being characterized for the development of diagnostic DNA probes for Toxoplasma.
Savva
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Department of Biochemistry and Physiology, University of Reading, Great Britain.
D000818:Animals; D004247:DNA / Q000302:isolation & purification; D015342:DNA Probes; D004926:Escherichia coli / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D015183:Restriction Mapping; D014122:Toxoplasma / Q000235:genetics*; D014170:Transformation, Genetic
D015342:DNA Probes; D004247:DNA