Clathrin: a role in the intracellular retention of a Golgi membrane protein.
Yeast mutants deficient in the clathrin heavy chain secrete a precursor form of the alpha-factor, a peptide-mating pheromone. Analysis of this defect indicates that the endoprotease Kex2p, which is responsible for initiating proteolytic maturation of the alpha-factor precursor in the Golgi apparatus, is unexpectedly present at the plasma membrane in mutant cells. This result suggest that clathrin is required for the retention of Kex2p in the Golgi apparatus.
Payne
G S
GS
Department of Biological Chemistry, UCLA School of Medicine 90024.
Schekman
R
R
D002451:Cell Compartmentation; D002966:Clathrin / Q000502:physiology*; D006056:Golgi Apparatus / Q000502:physiology*; D007425:Intracellular Membranes / Q000502:physiology*; D000072235:Mating Factor; D008565:Membrane Proteins / Q000502:physiology*; D010447:Peptide Hydrolases / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D011498:Protein Precursors / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D012441:Saccharomyces cerevisiae
D002966:Clathrin; D008565:Membrane Proteins; D010455:Peptides; D011498:Protein Precursors; D000072235:Mating Factor; D010447:Peptide Hydrolases