title: Membrane self-digestion during erythrocyte storage.
abstract: The effect of storage of erythrocytes (in vitro aging) on the self-digestion of erythrocyte membrane proteins was studied by analysis of membrane protein electropherograms. Incubation of bovine erythrocytes in medium 199 for 7 to 31 days resulted in a progressive decrease in the self-digestion rate of band 3 and a less regular decrease in the rate of self-digestion of spectrin. This effect may be explained as a result of a decrease of membrane protease activity and an increase in the proteolytic susceptibility of membrane proteins.
authors.affiliation: Laboratory of Biophysics, Development and Aging, University of Lódź, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Gaczyńska
authors.forename: G
authors.initials: G
authors.lastname: Bartosz
authors.forename: J
authors.initials: J
authors.lastname: Rosin
mesh_terms: D000818:Animals; D001793:Blood Preservation / Q000379:methods; D002417:Cattle; D002470:Cell Survival; D004910:Erythrocyte Membrane / Q000032:analysis / Q000378:metabolism*; D008565:Membrane Proteins / Q000032:analysis / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism; D013049:Spectrin / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; D013049:Spectrin; D010447:Peptide Hydrolases