title: Inhibition of human macrophage colony formation by interleukin 4.
abstract: We investigated the effects of human rIL-4 on in vitro hematopoiesis. A profound inhibition of macrophage colony formation by IL-4 was observed, whereas colony growth of other lineages was not affected. Inhibition of macrophage colony growth was not restricted to GM-CSF-induced colony growth but was also present in cultures stimulated with M-CSF. This inhibition was not only observed in cultures of light density bone marrow cells, but also in cultures of monocyte- and T lymphocyte-depleted bone marrow cells. Since a similar inhibition was observed in cultures of CD34+HLA-DR+-enriched bone marrow cells, a direct action of IL-4 on monocyte-committed progenitor cells is suggested.
authors.affiliation: Department of Hematology, Leiden University Medical Center, The Netherlands.
authors.forename: J H
authors.initials: JH
authors.lastname: Jansen
authors.forename: G J
authors.initials: GJ
authors.lastname: Wientjens
authors.forename: W E
authors.initials: WE
authors.lastname: Fibbe
authors.forename: R
authors.initials: R
authors.lastname: Willemze
authors.forename: H C
authors.initials: HC
authors.lastname: Kluin-Nelemans
mesh_terms: D000938:Antigen-Presenting Cells / Q000276:immunology; D001854:Bone Marrow Cells; D003115:Colony-Stimulating Factors / Q000037:antagonists & inhibitors; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006131:Growth Inhibitors; D006410:Hematopoiesis / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D015847:Interleukin-4; D007378:Interleukins / Q000494:pharmacology*; D016173:Macrophage Colony-Stimulating Factor; D008264:Macrophages / Q000502:physiology*; D011994:Recombinant Proteins
chemical_list: D003115:Colony-Stimulating Factors; D006131:Growth Inhibitors; D007378:Interleukins; D011994:Recombinant Proteins; D015847:Interleukin-4; D016173:Macrophage Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor