Rapid homogeneous enzyme immunoassay of plasma protein C.
A homogeneous EIA (H-EIA) technique for plasma protein C antigen using horseradish peroxidase (HRP) was developed. This technique, which avoids the bound/free separation and washing procedures and shortens the assay time to 30 min, has several advantages over the conventional EIA technique. The measurement was valid for 1.0% to 200% of the plasma protein C level and had intraassay coefficients of variation of 2.3-11.0%. The results of this assay correlated well with those of conventional sandwich EIA (x) with a regression equation of y = 0.782x + 6.637, r = 0.911 (n = 59). This method, which may also be applicable to automatic assay systems, is considered to be suited for clinical laboratory use.
Suzuki
K
K
Department of Laboratory Medicine, Mie University School of Medicine, Tsu, Japan.
Hayashi
T
T
Koyama
M
M
Yoshimura
T
T
Shimamoto
M
M
Kimura
N
N
Kita
M
M
D000941:Antigens / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D008107:Liver Diseases / Q000097:blood; D011486:Protein C / Q000032:analysis* / Q000276:immunology; D012016:Reference Values; D015203:Reproducibility of Results; D013924:Thrombophlebitis / Q000097:blood; D014859:Warfarin / Q000494:pharmacology
D000941:Antigens; D011486:Protein C; D014859:Warfarin