Studies on the efficiency of absorbed bovine PPD in tuberculin and serological tests.
The passive haemagglutination, enzyme-linked immunosorbent assay and indirect fluorescent antibody tests were applied to study the non-specific reactions in experimentally infected guinea pigs and tuberculin positive bovines. These cross-reactions were greatly decreased after absorption of either sera with avian PPD or bovine PPD antigen with anti-avian PPD serum. The use of both absorbed sera and antigen raised the specificity of PHA and ELISA to 100%. The use of absorbed sera rendered the IFA specific in 95%. The absorption has reduced the sensitivity of ELISA, IFA and PHA by 14, 27 and 29%, respectively.
Hammam
H
H
Refai
M
M
Bisping
W
W
Kirpal
G
G
D000818:Animals; D002417:Cattle; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D006386:Hemagglutination Tests; D011237:Predictive Value of Tests; D014373:Tuberculin / Q000276:immunology*; D014374:Tuberculin Test / Q000662:veterinary*; D014380:Tuberculosis, Bovine / Q000175:diagnosis*
D014373:Tuberculin