A specific inhibition of induction of adaptive response by o-vanillin, a potent comutagen.
The present study revealed that potent comutagenic activity of o-vanillin in mutagenesis by N-methyl-N-nitrosourea (MNU) is a consequence of inhibition of the transcriptional promoting capacity of methylated O6-methylguanine-DNA methyltransferase (MGTase). As evidence of this, in the presence of o-vanillin, there were (i) dose-dependent decreases in MGTase induced in E. coli exposed to MNU, (ii) inhibition of ada gene promotion determined in the ada'-lacZ' assay system, and (iii) minimal inhibition of the methyl transfer capacity of MGTase in a subcellular system.
Takahashi
K
K
Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
Sekiguchi
M
M
Kawazoe
Y
Y
D001547:Benzaldehydes / Q000494:pharmacology*; D004260:DNA Repair / Q000187:drug effects*; D004357:Drug Synergism; D004790:Enzyme Induction / Q000187:drug effects; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D007536:Isomerism; D008770:Methylnitrosourea / Q000494:pharmacology; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism*; D009153:Mutagens; D019853:O(6)-Methylguanine-DNA Methyltransferase
D001547:Benzaldehydes; D009153:Mutagens; D008770:Methylnitrosourea; C100058:vanillin; D008780:Methyltransferases; D019853:O(6)-Methylguanine-DNA Methyltransferase