title: A role of secreted proteinase of Candida albicans for the invasion of chick chorio-allantoic membrane.
abstract: The invasion of Candida albicans strains into the chorio-allantoic membrane (CAM) of a developing chick was studied by light and electron microscopy. A proteinase-producing strain, NUM961, invaded into intact CAM, but proteinase-deficient strain NUM678 cells remained on the surface of the CAM with no evidence of damage to the host cells. However, NUM678 cells invaded into the ectoderm-damaged CAM, or proteinase-treated one. Electron microscopy revealed that treatment with purified Candida proteinase disorganized the ectoderm tissue by disrupting the intercellular junctions. These results suggest that Candida proteinase damages the CAM surface, which enables the invasion of the growing hyphae.
authors.affiliation: Laboratory of Medical Mycology, Nagoya University School of Medicine.
authors.forename: I
authors.initials: I
authors.lastname: Kobayashi
authors.forename: Y
authors.initials: Y
authors.lastname: Kondoh
authors.forename: K
authors.initials: K
authors.lastname: Shimizu
authors.forename: K
authors.initials: K
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D002176:Candida albicans / Q000201:enzymology* / Q000648:ultrastructure; D002177:Candidiasis / Q000473:pathology / Q000662:veterinary*; D002645:Chickens / Q000382:microbiology*; D002823:Chorion / Q000382:microbiology* / Q000473:pathology / Q000648:ultrastructure; D004475:Ectoderm / Q000382:microbiology / Q000473:pathology / Q000648:ultrastructure; D010447:Peptide Hydrolases / Q000378:metabolism*; D011201:Poultry Diseases / Q000382:microbiology*
chemical_list: D010447:Peptide Hydrolases