Time-dependent inactivation of glucose-6-phosphate dehydrogenase from yeast by aluminum.
Aluminum inhibited yeast glucose-6-phosphate dehydrogenase (EC 1.1.1.49) by a pseudo-first-order reaction. The inhibition was proportional to the incubation time and the concentration of aluminum. Double reciprocal plots gave a straight line with a kinact of 8.3 min-1 and indicated the presence of a binding step prior to inhibition. The kinetic study showed that 1 mol of aluminum was bound per mol of enzyme subunit.
Cho
S W
SW
Department of Biochemistry, University of Tennessee, Knoxville 37996-0840.
Joshi
J G
JG
D000535:Aluminum / Q000633:toxicity*; D005954:Glucosephosphate Dehydrogenase / Q000037:antagonists & inhibitors*; D007700:Kinetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013997:Time Factors
D000535:Aluminum; D005954:Glucosephosphate Dehydrogenase