Purification of Plasmodium falciparum exoantigens by spun-column chromatography and by inverse affinity chromatography.
Exoantigens released into the medium during the in vitro culture of Plasmodium falciparum were purified to obtain a better characterization. This purification was done in two steps. First the crude culture medium was passed through a cationic exchange gel on a spun-column. This preliminary step is efficient and rapid. The serum contaminants were removed from the effluent by an inverse affinity chromatography with, as adsorbant, anti-human serum from the horse. The quality of the antigens obtained was analysed by metabolic radioactive labelling with tritiated leucine and by exclusion high pressure liquid chromatography, and the antigenicity measured by ELISA.
Thelu
J
J
Laboratoire de Parasitologie et Pathologie Exotique, Faculté de Médecine, Université de Grenoble, France.
Chumpitazi
B
B
Ambroise-Thomas
P
P
D000818:Animals; D000953:Antigens, Protozoan / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D008970:Molecular Weight; D010963:Plasmodium falciparum / Q000276:immunology*
D000953:Antigens, Protozoan