title: Organic anion and cation transport in crab urinary bladder.
abstract: Crab urinary bladder, a simple, flat-sheet epithelium, is structurally and functionally analogous to vertebrate renal proximal tubule. Like proximal tubule, crab bladder plays an important role in the excretion of potentially toxic, charged metabolites and xenobiotics. Bladders from Cancer borealis secrete monovalent, organic anions and cations in vivo and in vitro. For organic cations, secretion is a two-step process, with mediated and energetically downhill uptake into cells at the serosal membrane and uphill exit at the luminal membrane. The uptake step may be driven by the electrical potential difference across the serosal membrane, the luminal step by organic cation-proton exchange. Monovalent organic anions are also secreted by a separate two-step process. Recent experiments with intact bladder tissue and isolated membrane vesicles show that (as in mammalian proximal tubule) uphill serosal uptake can be coupled indirectly to the Na+ gradient. Organic anion (p-aminohippurate; PAH) uptake is driven by exchange for certain divalent organic anions, e.g., glutarate and alpha-ketoglutarate. The divalent anion gradient (in greater than out) is in turn maintained by Na+-coupled divalent uptake. The PAH exist step at the luminal membrane is mediated and downhill; it may involve anion exchange.
authors.affiliation: Laboratory of Cellular and Molecular Pharmacology, National Institutes of Health, Research Triangle Park, North Carolina 27709.
authors.forename: D S
authors.initials: DS
authors.lastname: Miller
authors.forename: P M
authors.initials: PM
authors.lastname: Smith
authors.forename: J B
authors.initials: JB
authors.lastname: Pritchard
mesh_terms: D000818:Animals; D000838:Anions / Q000378:metabolism*; D001692:Biological Transport; D001696:Biomechanical Phenomena; D003386:Brachyura / Q000378:metabolism*; D002412:Cations / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism; D001743:Urinary Bladder / Q000378:metabolism*
chemical_list: D000838:Anions; D002412:Cations