Colonization of the intestine of turkey embryos exposed to Mycoplasma iowae.
Eight-day-old turkey embryos were inoculated into the yolk sac with 3 X 10(5) colony-forming units of Mycoplasma iowae strain D112 in order to study the growth-depressing effect, the histopathological changes, and colonization of the intestinal tract. The embryo: egg weight ratio was significantly lower in the inoculated eggs than in controls. Histologically, there were infiltrations in parenchymatous organs and chorioallantoic membranes with heterophilic granulocytes. M. Iowae was demonstrated on the intestinal mucosa by antibody fluorescent microscopy and by transmission electron microscopy. Attaching mycoplasmas had a distinct morphology; the segment in contact with enterocytes was cone-shaped and had finely granulated cytoplasma which was abruptly separated from the distal coarsely granulated area. We conclude that M. iowae has a predilection for the intestinal tract of avian hosts.
Mirsalimi
S M
SM
Department of Pathology, Ontario Veterinary College, University of Guelph, Canada.
Rosendal
S
S
Julian
R J
RJ
D000818:Animals; D004625:Embryo, Nonmammalian / Q000382:microbiology; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D007421:Intestine, Small / Q000196:embryology / Q000382:microbiology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D009174:Mycoplasma / Q000254:growth & development* / Q000648:ultrastructure; D009175:Mycoplasma Infections / Q000196:embryology / Q000382:microbiology / Q000662:veterinary*; D011201:Poultry Diseases / Q000196:embryology / Q000382:microbiology*; D013997:Time Factors; D014422:Turkeys / Q000196:embryology / Q000382:microbiology*; D015017:Yolk Sac / Q000382:microbiology