title: Cytobrush in collection of cervical specimens for detection of Chlamydia trachomatis.
abstract: Methods of collecting endocervical samples for the detection of Chlamydia trachomatis were evaluated. We compared Calgiswab and cytobrush for isolation in cell culture and Dacron swab and cytobrush for direct fluorescent-antibody (DFA) testing for 632 females attending a sexually transmitted disease clinic. An additional specimen for enzyme immunoassay (EIA) was also collected. True-positives were identified as tissue culture positive and/or both DFA and EIA positive. Use of the cytobrush significantly improved the sensitivity of both the culture (69% with swab and 100% with cytobrush) and DFA testing (68% with swab and 85% with cytobrush). The EIA sensitivity was 85%. The specificity of each test was greater than or equal to 98%. The cytobrush appears to be the superior method for the collection of cervical samples from nonpregnant women.
authors.affiliation: Department of Laboratory Medicine, University of California, San Francisco 94143.
authors.forename: J
authors.initials: J
authors.lastname: Moncada
authors.forename: J
authors.initials: J
authors.lastname: Schachter
authors.forename: M
authors.initials: M
authors.lastname: Shipp
authors.forename: G
authors.initials: G
authors.lastname: Bolan
authors.forename: J
authors.initials: J
authors.lastname: Wilber
mesh_terms: D002584:Cervix Uteri / Q000382:microbiology*; D002690:Chlamydia Infections / Q000382:microbiology*; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D011237:Predictive Value of Tests; D012749:Sexually Transmitted Diseases / Q000382:microbiology*; D013048:Specimen Handling / Q000379:methods*