Removal of RNA during protein concentration by means of enzyme membrane.
Immobilization of RNase in PVC ultrafiltration membranes was carried out. The obtained membranes were used for concentration of BSA solution, RNA being simultaneously removed. The yield of RNA hydrolysis was found to be controlled by the initial concentration of RNA in feed solution. The protein affected enzyme action as a result of its adsorption on the membrane surface at the beginning of ultrafiltration, whereas it did not inhibit RNase activity during the process.
Rucka
M
M
Institute of Organic and Physical Chemistry, Technical University of Wroclaw, Poland.
Turkiewicz
B
B
D004800:Enzymes, Immobilized; D006863:Hydrogen-Ion Concentration; D008567:Membranes, Artificial; D012331:RNA, Fungal / Q000302:isolation & purification*; D012260:Ribonucleases; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012710:Serum Albumin, Bovine / Q000302:isolation & purification*; D014462:Ultrafiltration / Q000295:instrumentation*
D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D012331:RNA, Fungal; D012710:Serum Albumin, Bovine; D012260:Ribonucleases