The influence of albumin on palmitate transport in the isolated perfused rat liver.
Unidirectional fluxes (influx and efflux) and the net flux of palmitate across the hepatocyte membrane were measured in the intact rat liver employing the multiple indicator dilution technique. At albumin concentrations in the range between 0.1 and 0.5 mM the influx rate was 2.3 times greater than that of the net flux. The rate of efflux was somewhat higher than the net flux, indicating that palmitate undergoes an exchange process across the liver cell membranes with efflux to the extracellular albumin site being significant. At lower albumin concentrations, however, the influx/net flux ratio approached unity, implying that transport becomes a rate-limiting factor for metabolism.
Ferraresi-Filho
O
O
Laboratório de Metabolismo Hepático, Universidade Estadual de Maringá, Brasil.
Ferraresi
M L
ML
Constantin
J
J
Ishii-Iwamoto
E L
EL
Schwab
A J
AJ
Bracht
A
A
D000418:Albumins / Q000378:metabolism*; D000818:Animals; D002463:Cell Membrane Permeability; D007201:Indicator Dilution Techniques; D008099:Liver / Q000378:metabolism*; D010168:Palmitates / Q000493:pharmacokinetics*; D010169:Palmitic Acids / Q000493:pharmacokinetics*; D051381:Rats; D013995:Time
D000418:Albumins; D010168:Palmitates; D010169:Palmitic Acids