title: Evaluation of microparticle enzyme immunoassays for immunoglobulins G and M to rubella virus and Toxoplasma gondii on the Abbott IMx automated analyzer.
abstract: The ability of the Abbott IMx automated analyzer to detect immunoglobulin G (IgG) and IgM antibodies to rubella virus and to Toxoplasma gondii was compared with the abilities of RUBAZYME, RUBAZYME-M, ABBOTT TOXO-G enzyme immunoassay, and ABBOTT TOXO-M enzyme immunoassay, respectively. Specimens that produced discordant results were evaluated by RUBACELL II, Behring Enzygnost-Rubella enzyme-linked immunosorbent assay, Behring Enzygnost Toxoplasmosis/IgG, and bioMerieux Toxo-ISAGA (immunosorbent agglutination assay), respectively. After resolution of discordant results, IMx Rubella IgG, IMx Rubella IgM, IMx Toxo IgG, and IMx Toxo IgM antibody assays had sensitivities of 99.9, 100, 98.0, and 100%; specificities of 98.9, 99.0, 97.5, and 98.7%; and accuracies of 99.8, 99.3, 97.8, and 98.8%, respectively.
authors.affiliation: Diagnostics Division, Abbott Laboratories, Abbott Park, Illinois 60064.
authors.forename: L E
authors.initials: LE
authors.lastname: Schaefer
authors.forename: J W
authors.initials: JW
authors.lastname: Dyke
authors.forename: F D
authors.initials: FD
authors.lastname: Meglio
authors.forename: P R
authors.initials: PR
authors.lastname: Murray
authors.forename: W
authors.initials: W
authors.lastname: Crafts
authors.forename: A C
authors.initials: AC
authors.lastname: Niles
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis; D000914:Antibodies, Viral; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis*; D011237:Predictive Value of Tests; D012409:Rubella / Q000276:immunology*; D014122:Toxoplasma / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan; D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; C040901:rubella antibodies