Expression of a muscle-type alpha-actinin cDNA clone in non-muscle cells.
We have previously isolated a chick smooth muscle-type alpha-actinin cDNA clone (C17) from a chick embryo fibroblast cDNA library. As part of an investigation into a possible role for a muscle isoform of alpha-actinin in non-muscle cells, we have cloned C17 into a eucaryotic expression vector, pKCR3, and examined the distribution of the expressed protein in non-muscle, monkey COS cells. We report here that the muscle isoform of chick alpha-actinin encoded by C17, was found in focal contacts and periodically distributed along actin filaments.
Jackson
P
P
Department of Biochemistry, University of Leicester, United Kingdom.
Smith
G
G
Critchley
D R
DR
D000185:Actinin / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000096:biosynthesis; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D007106:Immune Sera; D010957:Plasmids; D011233:Precipitin Tests; D014162:Transfection
D007106:Immune Sera; D000185:Actinin; D004247:DNA