Monoclonal antibody production against a subcellular fraction of vegetal pole cytoplasm containing the germ plasm of Xenopus 2-cell eggs.
In attempts to understand the molecular nature of substances localized in the germ plasm (GP), monoclonal antibodies against a subcellular fraction of vegetal pole cytoplasm containing the GP of Xenopus 2-cell eggs were produced. The monoclonal antibody produced by the hybridoma cell line (designated as no 48) reacted specifically with the GP of embryos at the cleavage stages. Beyond the gastrula stage, the antibody reacted with not only the GP of germ line cells but also the cytoplasm of somatic cells. By immunoblotting analysis with two-dimensional (2-D) gel the antibody was shown to react with two protein spots of approx. 40 kDa, with a pI of 6.0-6.5.
Nakazato
S
S
Department of Biology, Faculty of Science, Osaka City University, Japan.
Ikenishi
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000302:isolation & purification; D000941:Antigens / Q000302:isolation & purification; D002459:Cell Fusion; D002498:Centrifugation; D003593:Cytoplasm / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional; D005455:Fluorescent Antibody Technique; D007114:Immunization; D015151:Immunoblotting; D011817:Rabbits; D013347:Subcellular Fractions / Q000032:analysis*; D014981:Xenopus / Q000196:embryology*; D015053:Zygote / Q000032:analysis* / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D000941:Antigens