Effect of insulin on glucose transporter distribution in white fat cells from hypophysectomized rats.
Glucose transport in white fat cells from hypophysectomized rats is increased and unresponsive to insulin. The goal of this study was to explain this observation. The number of glucose transporters, as determined by D-glucose-inhibitible cytochalasin B binding, in the plasma membranes from fat cells of hypophysectomized rats is: (1) elevated, (2) not increased by insulin, and (3) the same as in plasma membranes from insulin-stimulated fat cells of control rats. In microsomal membranes from fat cells of hypophysectomized rats the number of glucose transporters is: (1) smaller than in basal and insulin-stimulated fat cells from control rats, and (2) not changed by insulin.
Keller
S
S
Department of Medicine, University Hospital Zurich, Switzerland.
Zapf
J
J
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D002451:Cell Compartmentation / Q000187:drug effects; D002462:Cell Membrane / Q000378:metabolism; D003571:Cytochalasin B / Q000378:metabolism; D007016:Hypophysectomy; D007328:Insulin / Q000494:pharmacology*; D008297:Male; D008861:Microsomes / Q000378:metabolism; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D051381:Rats
D007328:Insulin; D009004:Monosaccharide Transport Proteins; D003571:Cytochalasin B