title: Overproduction, affinity purification and characterization of 65-kDa protein of Mycobacterium leprae in Escherichia coli.
abstract: The 65-kDa protein of Mycobacterium leprae was produced in an Escherichia coli strain carrying a plasmid harboring the recloned gene coding for the protein. The protein was purified through affinity chromatography prepared with the IgG fraction of a monoclonal antibody which was prepared against the 65-kDa protein. The purified 65-kDa protein also reacted immunologically with the monoclonal antibody IIIE9, which recognizes the epitope for M. leprae, prepared by Buchanan, et al. BALB/c mice were inoculated with M. leprae and 4 months later were skin tested with the purified 65-kDa protein. Gross changes were observed at the skin-test site. The role of the protein in protective immunity against M. leprae foot pad infection in mice was also studied.
authors.affiliation: Research Institute for Microbial Diseases, Osaka University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Nomaguchi
authors.forename: M
authors.initials: M
authors.lastname: Matsuoka
authors.forename: K
authors.initials: K
authors.lastname: Kohsaka
authors.forename: A
authors.initials: A
authors.lastname: Nakata
authors.forename: T
authors.initials: T
authors.lastname: Ito
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000378:metabolism*; D005260:Female; D006968:Hypersensitivity, Delayed; D051379:Mice; D008807:Mice, Inbred BALB C; D009166:Mycobacterium leprae / Q000276:immunology*
chemical_list: D001426:Bacterial Proteins