pepM is an essential gene in Salmonella typhimurium.
The pepM gene of Salmonella typhimurium codes for a methionine-specific aminopeptidase that removes N-terminal methionine residues from proteins. This gene was inactivated in vitro by the insertion of a DNA fragment coding for kanamycin resistance. The inactivated gene could not replace the wild-type chromosomal pepM gene unless another functional copy was present in the cell. The lethal effect of the pepM insertion was not a result of polarity on any gene downstream, nor was it affected by the presence or absence of other peptidases.
Miller
C G
CG
Department of Molecular Biology and Microbiology, Case Western Reserve University, Cleveland, Ohio 44106.
Kukral
A M
AM
Miller
J L
JL
Movva
N R
NR
D000626:Aminopeptidases / Q000235:genetics*; D015139:Blotting, Southern; D003433:Crosses, Genetic; D005796:Genes; D005798:Genes, Bacterial; D063208:Methionyl Aminopeptidases; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012486:Salmonella typhimurium / Q000201:enzymology / Q000235:genetics*; D014161:Transduction, Genetic
D000626:Aminopeptidases; D063208:Methionyl Aminopeptidases