Centrifugal enhancement of human immunodeficiency virus (HIV) and human herpesvirus type 6 (HHV-6) infection in vitro.
The effect of centrifugal inoculation of human immunodeficiency virus (HIV) and human herpesvirus-6 (HHV-6) on the infectivity of the viruses for cell cultures was examined. Three HIV-1 strains, ARV-2, HTLV-IIIb and a local isolate, WA-46c, were tested in peripheral blood lymphocytes, HUT-78, H9 and MT-2 cells. The HHV-6 strain was a local isolate and was studied only in peripheral blood lymphocyte cultures. Centrifugal inoculation of the viruses at a force of 2500 x g for 60 min, enhanced HIV-1 infectivity by a factor of about 10-fold in all cell cultures tested. Infectivity was increased about 100-fold for HHV-6.
Pietroboni
G R
GR
Virus Laboratory, Combined Microbiology Service, Queen Elizabeth II Medical Centre, Nedlands, Australia.
Harnett
G B
GB
Bucens
M R
MR
D002460:Cell Line; D002498:Centrifugation / Q000379:methods; D005455:Fluorescent Antibody Technique; D006678:HIV / Q000472:pathogenicity* / Q000502:physiology; D006564:Herpesviridae / Q000472:pathogenicity* / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000382:microbiology; D014162:Transfection; D014773:Virology / Q000379:methods*; D014779:Virus Replication