title: SHV-5, a novel SHV-type beta-lactamase that hydrolyzes broad-spectrum cephalosporins and monobactams.
abstract: SHV-5 (pI 8.2), a novel broad-spectrum beta-lactamase encoded by a ca. 150-kilobase plasmid, was found in Klebsiella pneumoniae 160. SHV-5 beta-lactamase caused decreased susceptibility to most penicillins, cephalosporins, and monobactams, except imipenem and compounds which have a C6 or C7 alpha-methoxy substituent. beta-Lactamase inhibitors (clavulanic acid, sulbactam, and tazobactam) inhibited its activity and showed a synergistic effect when associated with different hydrolyzable beta-lactam compounds. Hybridization studies suggested that this enzyme may be related to, or derived from, the SHV enzyme. Increased MICs of cephamycins and temocillin associated with a decreased synergistic effect of the inhibitors on K. pneumoniae 160 might be linked to a decrease in two outer membrane proteins.
authors.affiliation: Laboratoire de Microbiologie Médicale, Hospital Broussais, Paris, France.
authors.forename: L
authors.initials: L
authors.lastname: Gutmann
authors.forename: B
authors.initials: B
authors.lastname: Ferré
authors.forename: F W
authors.initials: FW
authors.lastname: Goldstein
authors.forename: N
authors.initials: N
authors.lastname: Rizk
authors.forename: E
authors.initials: E
authors.lastname: Pinto-Schuster
authors.forename: J F
authors.initials: JF
authors.lastname: Acar
authors.forename: E
authors.initials: E
authors.lastname: Collatz
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism; D002511:Cephalosporins / Q000378:metabolism*; D004269:DNA, Bacterial / Q000096:biosynthesis; D007711:Klebsiella pneumoniae / Q000201:enzymology*; D008826:Microbial Sensitivity Tests; D008997:Monobactams / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases / Q000096:biosynthesis / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001425:Bacterial Outer Membrane Proteins; D002511:Cephalosporins; D004269:DNA, Bacterial; D008997:Monobactams; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases