title: [A study of the intracellular fate of recombinant human interleukin 2 in Escherichia coli].
abstract: The expression of the human IL-2 recombinant gene in E. coli cells was studied. The processes which take place during thermo-induced expression and effect the state of the product were investigated. Experimental data on the membrane localisation of IL-2, the formation of aggregates (inclusion bodies) and polymers were obtained. It was determined that temperature significantly influence the kinetics of the processes of intracellular IL-2 production and IL-2 stability. It is supposed that the cell membrane state plays a determining role in these processes via temperature mediation. Thus, the formation of inclusion bodies described for a number of E. coli recombinant strains is probably stipulated not only by recombinant polypeptides properties, but also by cellular interactions.
authors.forename: A B
authors.initials: AB
authors.lastname: Meriin
authors.forename: V A
authors.initials: VA
authors.lastname: Ivaniushina
authors.forename: A I
authors.initials: AI
authors.lastname: Dobkin
authors.forename: V I
authors.initials: VI
authors.lastname: Ogarkov
mesh_terms: D001345:Autoradiography; D002462:Cell Membrane / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D007376:Interleukin-2 / Q000235:genetics* / Q000378:metabolism; D007700:Kinetics; D010957:Plasmids; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013696:Temperature
chemical_list: D007376:Interleukin-2; D011994:Recombinant Proteins
vernacular_title: Issledovanie vnutrikletochnoĭ sud'by rekombinantnogo chelovecheskogo interleĭkina 2 v Escherichia coli.