N alpha acetylation is required for normal growth and mating of Saccharomyces cerevisiae.
Acetylation is the most frequently occurring chemical modification of the alpha-NH2 group of eucaryotic proteins and is catalyzed by N alpha-acetyltransferase. The yeast enzyme is encoded by the AAA1 (amino-terminal alpha-amino acetyltransferase) gene. A null mutation (aaa1-1) created by gene replacement, while not lethal, slows cell growth and results in heterogeneous colony morphology. In comparison with wild-type cells, aaa1-1/aaa1-1 diploids cannot enter stationary phase, are sporulation defective, and are sensitive to heat shock. In addition, the aaa1-1 mutation specifically reduces mating functions of MATa cells. These results indicate that N alpha acetylation plays a crucial role in yeast cell growth and mating.
Lee
F J
FJ
Department of Molecular Biology, Massachusetts General Hospital, Boston.
Lin
L W
LW
Smith
J A
JA
D000107:Acetylation; D000123:Acetyltransferases / Q000235:genetics*; D005800:Genes, Fungal; D005838:Genotype; D006358:Hot Temperature; D009154:Mutation; D063206:N-Terminal Acetyltransferases; D010675:Pheromones / Q000096:biosynthesis; D010957:Plasmids; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D013045:Species Specificity
D010675:Pheromones; D000123:Acetyltransferases; D063206:N-Terminal Acetyltransferases