title: Elevation of c-myc protein by DNA strand breakage.
abstract: The intracellular level of the c-myc protein is elevated in a dose and time dependent manner by DNA strand breakage. Lesions introduced by either alkylating agents or gamma irradiation are capable of stimulating increased production of c-myc protein. In addition, inhibition of DNA strand break rejoining maintains the level of the c-myc protein in an elevated state, whilst inhibition of DNA replication does not cause an increase in c-myc protein. We conclude that chromatin perturbation via DNA strand breakage both increases the endogenous amounts of the c-myc protein and maintains its elevated level.
authors.affiliation: Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, UK.
authors.forename: N F
authors.initials: NF
authors.lastname: Sullivan
authors.forename: A E
authors.initials: AE
authors.lastname: Willis
mesh_terms: D000477:Alkylating Agents / Q000494:pharmacology; D002460:Cell Line; D004249:DNA Damage; D004261:DNA Replication / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007700:Kinetics; D011505:Protein-Tyrosine Kinases / Q000096:biosynthesis*; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000096:biosynthesis*; D016271:Proto-Oncogene Proteins c-myc; D011519:Proto-Oncogenes
chemical_list: D000477:Alkylating Agents; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D011505:Protein-Tyrosine Kinases