Direct HLA typing by rosetting with immunomagnetic beads coated with specific antibodies.
Paramagnetic monodisperse polymer beads were coated with purified human hybridoma antibodies specific for polymorphic HLA determinants. The mAb-coated beads bound specifically to cells from individuals with the relevant HLA type. The rosettes formed by beads and cells were isolated with a magnet. Rosette formation was evaluated microscopically and used as criterion for positive typing. By this rosette assay, fast and reliable HLA typing of whole blood was possible.
Hansen
T
T
Institute of Medical Biology, University of Tromsø, Norway.
Hannestad
K
K
D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D006681:HLA-D Antigens; D015395:Histocompatibility Antigens Class I; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D007158:Immunologic Techniques; D012397:Rosette Formation
D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D006681:HLA-D Antigens; D015395:Histocompatibility Antigens Class I