title: Isolation and characterization of shrew (Suncus murinus) liver alcohol dehydrogenases.
abstract: 1. Starch gel electrophoresis of adult shrew (Suncus murinus) liver extracts revealed five forms of alcohol dehydrogenase (ADH 1-5) and four of them were purified. 2. ADH-4 and ADH-5 resemble human class I ADH in terms of electrophoretic mobility, substrate specificity and sensitivity to pyrazole inhibition. 3. ADH-2 does not belong to any of the three classes of human ADHs but rather with catalytic properties similar to those of the class B ADH found in guinea pig liver. 4. ADH-1 prefers secondary alcohol over primary alcohol substrates and between the enantiomers tested, the enzyme favors the S isomers.
authors.affiliation: Department of Biochemistry, Chinese University of Hong Kong, Shatin, New Territories.
authors.forename: W M
authors.initials: WM
authors.lastname: Keung
authors.forename: Y W
authors.initials: YW
authors.lastname: Ho
authors.forename: W P
authors.initials: WP
authors.lastname: Fong
authors.forename: C Y
authors.initials: CY
authors.lastname: Lee
mesh_terms: D000429:Alcohol Oxidoreductases / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000378:metabolism; D000818:Animals; D000077604:Fomepizole; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D011720:Pyrazoles / Q000494:pharmacology; D012788:Shrews / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D007527:Isoenzymes; D011720:Pyrazoles; D000077604:Fomepizole; D000429:Alcohol Oxidoreductases