title: Identification of Tritrichomonas foetus in sections of bovine placental tissue with monoclonal antibodies.
abstract: Sections of bovine placenta from cases of bovine trichomoniasis were examined for the presence of Tritrichomonas foetus by standard histological methods using phase-contrast microscopy and by indirect immunofluorescence assay (IFA) employing monoclonal antibodies (mAbs) specific for T. foetus. Parasites were identified readily in deparaffinized tissue up to 4 yr old by IFA with 2 mAbs previously shown to bind to the surface of living T. foetus. These results indicated that the IFA provided a rapid and specific method of identifying T. foetus in tissue sections as compared to standard histological methods.
authors.affiliation: Veterinary Research Laboratory, Montana State University, Bozeman 59717.
authors.forename: D E
authors.initials: DE
authors.lastname: Burgess
authors.forename: K F
authors.initials: KF
authors.lastname: Knoblock
mesh_terms: D000034:Abortion, Veterinary / Q000469:parasitology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology*; D003429:Cross Reactions; D005260:Female; D005455:Fluorescent Antibody Technique; D008858:Microscopy, Phase-Contrast; D010920:Placenta / Q000469:parasitology*; D011247:Pregnancy; D011528:Protozoan Infections / Q000469:parasitology; D011529:Protozoan Infections, Animal; D014318:Tritrichomonas / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000911:Antibodies, Monoclonal