[Repair of a double-stranded gap in plasmid DNA in radiosensitive mutants of Saccharomyces cerevisiae: effectiveness and precision].
The repair of a double strand gap in plasmid DNA in radiosensitive mutants of Saccharomyces cerevisiae has been studied. The proportion of repair events resulting in the complete doublestrand gap recovery of the plasmid DNA has been found to be close to 100% in Rad+ cells. The mutation rad55 did not interfere in the doublestrand gap repair efficiency and accuracy. The mutant rad57 is capable of the effective doublestrand gap repair without restoration of the DNA sequence deleted by the gap. The mutation rad53 substantially inhibited the efficiency of the doublestrand gap repair but did not influence the accuracy of the repair. Plasmid DNA doublestranded gap repair is completely blocked by mutations rad50 and rad54.
Glazer
V M
VM
Glazunov
A V
AV
Tevzadze
G G
GG
Koltovaia
N A
NA
D004249:DNA Damage; D004260:DNA Repair; D004269:DNA, Bacterial / Q000528:radiation effects*; D004587:Electrophoresis, Agar Gel; D009154:Mutation; D010957:Plasmids; D011836:Radiation Tolerance; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000528:radiation effects
D004269:DNA, Bacterial
Reparatsiia dvunitevykh bresheĭ plazmidnoĭ DNK u radiochuvstvitel'nykh mutantov Saccharomyces cerevisiae: éffektivnost' i tochnost'.