title: Assignment of human desmin gene to band 2q35 by nonradioactive in situ hybridization.
abstract: A 3-kb DNA fragment, inserted in Bluescribe vector, was used to localize the desmin gene by in situ hybridization on human metaphase chromosomes. The probe was labelled by Bio-11-dUTP and detected by immunofluorescence. Subsequent R-banding indicated that the desmin gene is located in band 2q35.
authors.affiliation: URA 620 CNRS, Structure et Mutagenèse Chromosomiques, Institut Curie, Paris, France.
authors.forename: E
authors.initials: E
authors.lastname: Viegas-Péquignot
authors.forename: Z L
authors.initials: ZL
authors.lastname: Li
authors.forename: B
authors.initials: B
authors.lastname: Dutrillaux
authors.forename: F
authors.initials: F
authors.lastname: Apiou
authors.forename: D
authors.initials: D
authors.lastname: Paulin
mesh_terms: D001710:Biotin; D002874:Chromosome Mapping / Q000379:methods*; D002889:Chromosomes, Human, Pair 2 / Q000648:ultrastructure*; D015342:DNA Probes; D003893:Desmin / Q000235:genetics*; D005455:Fluorescent Antibody Technique; D006801:Humans; D009693:Nucleic Acid Hybridization
chemical_list: D015342:DNA Probes; D003893:Desmin; D001710:Biotin