An ARS/silencer binding factor also activates two ribosomal protein genes in yeast.
GFI is an abundant yeast DNA-binding protein, capable of binding to both ARS sequences and to the upstream regions of a number of nuclear genes coding for mitochondrial proteins (Dorsman et al., Nucl. Acids Res., 16 [1988] 7287-7301). GFI binding sites conform to the consensus RTCRYN5ACG, an element also present in the binding sites of factors designated SUF and TAF. These factors act as trans-activators of the constitutive transcription of the genes for ribosomal proteins S33 and L3 respectively. We now present evidence that GFI, TAF and SUF are probably the same protein. We speculate that one of the functions of GFI is the adjustment of the expression of a number of gene families to cell growth rate.
Dorsman
J C
JC
Department of Molecular Cell Biology, University of Amsterdam, The Netherlands.
Doorenbosch
M M
MM
Maurer
C T
CT
de Winde
J H
JH
Mager
W H
WH
Planta
R J
RJ
Grivell
L A
LA
D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005796:Genes; D005800:Genes, Fungal; D008745:Methylation; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D012269:Ribosomal Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D015345:Oligonucleotide Probes; D012269:Ribosomal Proteins