Wegener's granulomatosis autoantibodies identify a novel diisopropylfluorophosphate-binding protein in the lysosomes of normal human neutrophils.
Anti-neutrophil cytoplasmic autoantibodies (ANCA) specifically associated with Wegener's granulomatosis were found to be directed against a saline-soluble glycoprotein triplet that migrates on SDS gels as distinct bands of Mr 29,000, 30,500, and 32,000 and is present in the azurophilic granules. This antigen was specifically recognized by all cytoplasmic-staining (C)-ANCA-positive sera from patients with Wegener's disease. C-ANCA antigen bound [3H]diisopropylfluorophosphate, which indicates that it is a serine protease, but it could clearly be distinguished from the serine proteases elastase and cathepsin G. Stimulation of cytochalasin B-treated neutrophils with FMLP induced release of C-ANCA antigen. This indicates that in vivo C-ANCA might interact with the C-ANCA antigen after its release upon inflammatory stimulation. We further demonstrate that in some perinuclear staining (P-ANCA) patients' sera autoantibodies against other myeloid lysosomal enzymes can be detected, such as antimyeloperoxidase and antielastase. C-ANCA and P-ANCA thus represent a novel class of autoantibodies directed against myeloid lysosomal enzymes. The originally described Wegener-specific C-ANCA show an apparently uniform specificity for the 29,000 serine protease. In contrast, P-ANCA may recognize myeloperoxidase as well as elastase and/or other antigens.
Goldschmeding
R
R
Department of Immunological Haematology, Central Laboratory of the Netherlands, Red-Cross Blood Transfusion Service, Amsterdam.
van der Schoot
C E
CE
ten Bokkel Huinink
D
D
Hack
C E
CE
van den Ende
M E
ME
Kallenberg
C G
CG
von dem Borne
A E
AE
D000911:Antibodies, Monoclonal; D000941:Antigens / Q000276:immunology; D001323:Autoantibodies; D002352:Carrier Proteins / Q000032:analysis*; D056649:Cathepsin G; D002403:Cathepsins / Q000032:analysis; D003571:Cytochalasin B / Q000494:pharmacology; D003593:Cytoplasm / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D014890:Granulomatosis with Polyangiitis / Q000276:immunology*; D006801:Humans; D007163:Immunosorbent Techniques; D007531:Isoflurophate / Q000378:metabolism*; D019272:Leukocyte Elastase; D008247:Lysosomes / Q000201:enzymology*; D008970:Molecular Weight; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000276:immunology / Q000648:ultrastructure*; D010196:Pancreatic Elastase / Q000032:analysis; D012697:Serine Endopeptidases / Q000032:analysis*
D000911:Antibodies, Monoclonal; D000941:Antigens; D001323:Autoantibodies; D002352:Carrier Proteins; D007531:Isoflurophate; D003571:Cytochalasin B; D009240:N-Formylmethionine Leucyl-Phenylalanine; D002403:Cathepsins; D012697:Serine Endopeptidases; C534094:CTSG protein, human; D056649:Cathepsin G; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase