title: First crystallization of a phosphoenolpyruvate carboxylase from Escherichia coli.
abstract: Two different forms of crystal for a phosphoenolpyruvate carboxylase from Escherichia coli were obtained by the hanging-drop vapor diffusion technique, using polyethylene glycol 4000 as precipitant. The hexagonal crystal in space group P6(2)22 (or P6(4)22) has cell dimensions of a = 131 A and c = 325 A, whereas the orthorhombic crystal in space group I222 has a = 119 A, b = 252 A and c = 83 A. A tetrameric molecule (396,244 Mr), a subunit of which contains 883 amino residues, has a crystallographic 2 symmetry in the hexagonal crystal or 222 symmetry in the orthorhombic crystal, respectively.
authors.affiliation: Department of Applied Chemistry, Faculty of Engineering, Osaka University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Inoue
authors.forename: M
authors.initials: M
authors.lastname: Hayashi
authors.forename: M
authors.initials: M
authors.lastname: Sugimoto
authors.forename: S
authors.initials: S
authors.lastname: Harada
authors.forename: Y
authors.initials: Y
authors.lastname: Kai
authors.forename: N
authors.initials: N
authors.lastname: Kasai
authors.forename: K
authors.initials: K
authors.lastname: Terada
authors.forename: K
authors.initials: K
authors.lastname: Izui
mesh_terms: D002262:Carboxy-Lyases; D003460:Crystallization; D004926:Escherichia coli / Q000201:enzymology*; D010730:Phosphoenolpyruvate Carboxylase
chemical_list: D002262:Carboxy-Lyases; D010730:Phosphoenolpyruvate Carboxylase