Expression of human and viral ets genes in E. coli production of human ets-2-specific monoclonal antibodies.
Full-length and deletion variants of the ets proto-oncogene and v-ets oncogene of E26 virus were expressed in E. coli using the previously described expression vectors pANH1, pJL6 and pJLA16. The recombinant proteins were expressed at greater than 5% of the total cellular proteins and were characterized by Western blot analysis using ets-specific antipeptide antibodies. A number of monoclonal antibodies were raised against p35, the expressed product of the partial human ets-2 proto-oncogene construct. These monoclonal antibodies are highly specific and recognize the p56 ets-2 product from several human cell lines.
Seth
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A
Laboratory of Molecular Oncology, National Cancer Institute, Frederick, Maryland 21701-1013.
Watson
D K
DK
Fujiwara
S
S
Showalter
S D
SD
Papas
T S
TS
D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D015153:Blotting, Western; D004926:Escherichia coli / Q000235:genetics*; D005822:Genetic Vectors; D006801:Humans; D009857:Oncogenes; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D050783:Proto-Oncogene Proteins c-ets; D011519:Proto-Oncogenes; D012191:Retroviridae Proteins / Q000032:analysis / Q000235:genetics*; D015682:Retroviridae Proteins, Oncogenic; D014157:Transcription Factors
D000911:Antibodies, Monoclonal; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D012191:Retroviridae Proteins; D015682:Retroviridae Proteins, Oncogenic; D014157:Transcription Factors; C050379:oncogene proteins v-ets