[Testing of a modified Toxoplasma immunosorbent-adsorption assay (IgM-ISAGA) for the detection of Toxoplasma-specific IgM antibodies in the routine laboratory].
Testing of a modified Toxoplasma immunosorbent adsorption assay (IgM-ISAGA) for proving of Toxoplasma specific IgM antibodies. The IgM-ISAGA was assessed for the possibility of its application as a routine method in the serodiagnosis of toxoplasmosis and was compared with the IgM-IFT. On examination for specific on 136 sera, no false-positive results were obtained in the IgM-ISAGA in contrast to 5 false-positives in the IgM-IFT, due to the presence of rheumatoid factor. Its sensitivity was tested on 162 sera. The IgM-ISAGA reacted positive in 89 cases, the IgM-IFT in only 51. It was concluded that the IgM-ISAGA is a simple, sensitive and highly specific test applicable in smaller laboratories as a confirmatory reaction in the control of toxoplasmosis during pregnancy and in confirmation of connatal toxoplasmosis.
Lehmann
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D
Institut für Medizinische Mikrobiologie und Epidemiologie der Karl-Marx-Universität Leipzig, DDR.
D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis*; D007163:Immunosorbent Techniques; D007223:Infant; D007231:Infant, Newborn; D011247:Pregnancy; D014122:Toxoplasma / Q000276:immunology*; D014123:Toxoplasmosis / Q000175:diagnosis*; D014125:Toxoplasmosis, Congenital / Q000175:diagnosis*
D000913:Antibodies, Protozoan; D007075:Immunoglobulin M
Erprobung eines modifizierten Toxoplasma-Immunosorbent-Adsorptionsassays (IgM-ISAGA) zum Nachweis Toxoplasma-spezifischer IgM-Antikörper im Routinelabor.