Transcription of the dystrophin gene in Duchenne muscular dystrophy muscle.
Dystrophin is the recently discovered defective gene product in Duchenne and Becker muscular dystrophy (DMD and BMD). Dystrophin transcripts have been amplified and identified in diagnostic needle muscle biopsy samples using the polymerase chain reaction (PCR) procedure. Using 5'- and 3'-primers, dystrophin transcripts can be detected in both DMD and BMD muscle biopsies, on either side of defined deletions within the dystrophin gene.
Muntoni
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Jerry Lewis Muscle Research Centre, Department of Paediatrics and Neonatal Medicine, Royal Postgraduate Medical School, London, England.
Strong
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PN
D001483:Base Sequence; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D016189:Dystrophin; D004587:Electrophoresis, Agar Gel; D005784:Gene Amplification; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000235:genetics*; D009132:Muscles / Q000378:metabolism; D009136:Muscular Dystrophies / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D014158:Transcription, Genetic
D015342:DNA Probes; D016189:Dystrophin; D009124:Muscle Proteins; D012333:RNA, Messenger; D004247:DNA; D012321:DNA-Directed RNA Polymerases