In vitro expansion of the murine pluripotent hemopoietic stem cell population in response to interleukin 3 and interleukin 6. Application to bone marrow transplantation.
The synergistic action of interleukin 6 with interleukin 3 on the proliferation of a murine hemopoietic stem cell population in a short-term liquid culture system was examined by radioprotective assay. The numbers of colony-forming units in spleen (CFU-S), together with granulocyte/macrophage colony-forming units and viable nucleated cells, were found to increase markedly in culture in the presence of both IL-3 and IL-6, compared with the presence of IL-3 or IL-6 alone. The peak CFU-S value in response to the combination of IL-3 and IL-6 was obtained 6 days after culture initiation, exceeding 5-fold of the input value. Consistent with these data, marrow cells cultured with both IL-3 and IL-6 for 6 days were shown to have a much higher capability of rescuing lethally irradiated mice than did controls. The results may portend the potential clinical use of the combination of IL-3 and IL-6, in particular, in bone marrow transplantation.
Okano
A
A
Central Research Laboratories, Ajinomoto Co., Inc., Kawasaki, Japan.
Suzuki
C
C
Takatsuki
F
F
Akiyama
Y
Y
Koike
K
K
Ozawa
K
K
Hirano
T
T
Kishimoto
T
T
Nakahata
T
T
Asano
S
S
D000818:Animals; D016026:Bone Marrow Transplantation; D002455:Cell Division / Q000187:drug effects; D003114:Colony-Forming Units Assay; D004357:Drug Synergism; D006410:Hematopoiesis / Q000187:drug effects*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D066298:In Vitro Techniques; D007377:Interleukin-3 / Q000494:pharmacology*; D015850:Interleukin-6; D007378:Interleukins / Q000494:pharmacology*; D051379:Mice; D011828:Radiation Chimera
D007377:Interleukin-3; D015850:Interleukin-6; D007378:Interleukins