The mechanism of placental alkaline phosphatase induction in vitro.
The mechanism of placental alkaline phosphatase (PLAP) induction by prednisolone in a uterine cervical epidermoid cancer cell line SKG-IIIa was investigated in vitro by enzyme-cytochemistry, enzyme immunoassay, Northern and Southern blot analysis, and in situ hybridization. Enzyme-cytochemical alkaline phosphatase (ALP) staining and immunoassay revealed increased levels of PLAP (heat-stable ALP) in prednisolone-treated cells. Northern blot analysis and in situ hybridization showed increased amounts of PLAP mRNA. Southern blot analysis indicated that PLAP was not a product of an amplified or rearranged gene. These findings suggest that the induction of PLAP mRNA in SKG-IIIa cells by prednisolone in turn increased the levels of PLAP.
Nozawa
S
S
Department of Obstetrics and Gynecology, Keio University School of Medicine, Japan.
Narisawa
S
S
Iizuka
R
R
Fukasawa
T
T
Kohji
T
T
Nakane
P K
PK
Hirano
K
K
Millan
J L
JL
D000469:Alkaline Phosphatase / Q000096:biosynthesis* / Q000235:genetics; D015152:Blotting, Northern; D015139:Blotting, Southern; D002460:Cell Line; D004261:DNA Replication; D004790:Enzyme Induction; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D010920:Placenta / Q000201:enzymology* / Q000378:metabolism; D011239:Prednisolone / Q000494:pharmacology; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism
D012333:RNA, Messenger; D011239:Prednisolone; D000469:Alkaline Phosphatase