A simple method of bone marrow cryopreservation.
Mouse bone marrow cells (BMC) were frozen by the simple two step method and stored in liquid nitrogen. The reconstitutive potency of fresh and frozen BMC were compared. BMC-fresh and BMC-frozen had a similar potential of colony formation in spleen and reconstitution of lethally total body irradiated (TBI) mice.
Skórski
T
T
Department of Cytophysiology, Medical Centre of Postgraduate Education, Warsaw, Poland.
Kawalec
M
M
D000818:Animals; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D003114:Colony-Forming Units Assay; D005615:Freezing; D051379:Mice; D014021:Tissue Preservation / Q000379:methods*; D014916:Whole-Body Irradiation