Interaction of turkey complement with Escherichia coli isolated from turkeys.
The role of turkey complement in a serum bactericidal reaction was determined using serum-sensitive and serum-resistant Escherichia coli isolated from turkeys. Inactivation of complement by heating serum (56 C for 40 minutes) or by treating serum with 10 mM EDTA eliminated bactericidal activity. Serum-sensitive E coli organisms were killed by turkey serum treated with 10 mM ethylene glycol-bis-beta-(aminoethyl ether)-N,N,N',N'-tetraacetic acid and 5 mM MgCl2. Exposure of normal turkey serum to serum-sensitive or serum-resistant E coli resulted in equivalent reductions in hemolytic activity of serum. Treatment of serum-resistant E coli with antibody rendered the bacteria sensitive to bactericidal effects of normal turkey serum. Serum-sensitive E coli organisms were readily killed by an alternative complement pathway, serum-sensitive and serum-resistant E coli activated the complement system equally well, and antibody was required for complement-mediated killing of certain serum-resistant E coli organisms from turkeys.
Ellis
M G
MG
Department of Animal Science, Iowa State University, Ames 50011.
Arp
L H
LH
Lamont
S J
SJ
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001770:Blood Bactericidal Activity; D003165:Complement System Proteins / Q000276:immunology*; D004926:Escherichia coli / Q000276:immunology* / Q000302:isolation & purification; D014422:Turkeys / Q000276:immunology*
D000907:Antibodies, Bacterial; D003165:Complement System Proteins