title: In vivo administration of interleukin-1 inhibits glucose-stimulated insulin release.
abstract: Recombinant interleukin-1 beta (IL-1 beta) was administered intraperitoneally for 3 days to normal C57BL/6ByJ (B6) mice. The islets from IL-1-treated and control animals were isolated and glucose-stimulated insulin secretion studied in the perifusion system. The total islet insulin content and the ultrastructure of the islets isolated from the animals treated with IL-1 did not differ from those seen in control animals. However, glucose-stimulated insulin release was significantly impaired after 3 days of in vivo administration of IL-1, either 3 micrograms/animal/day or 0.3 micrograms/animal/day. The administration of IL-1 inhibited an acute phase of glucose-induced insulin release, whereas neither basal insulin secretion nor insulin release from 10-30 min of perifusion with glucose was impaired. There was an only partial (27%) and non-significant restoration of the insulin secretory response to glucose stimulation 4 days after discontinuation of IL-1 treatment. We conclude that IL-1 administered in vivo is capable of adversely affecting pancreatic islet response to glucose stimulation. After 3 days of administration, these changes are confined to the process of insulin release, with the islet cell morphology and total insulin content being unaffected.
authors.affiliation: Barbara Davis Center for Childhood Diabetes, Denver, CO.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: M
authors.initials: M
authors.lastname: Goodman
authors.forename: J
authors.initials: J
authors.lastname: Lumerman
authors.forename: K E
authors.initials: KE
authors.lastname: Sussman
authors.forename: R
authors.initials: R
authors.lastname: Dahl
authors.forename: K J
authors.initials: KJ
authors.lastname: Lafferty
authors.forename: B
authors.initials: B
authors.lastname: Draznin
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D005947:Glucose / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism*; D007329:Insulin Antagonists / Q000494:pharmacology; D000078790:Insulin Secretion; D007375:Interleukin-1 / Q000494:pharmacology*; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D051379:Mice; D008810:Mice, Inbred C57BL; D008854:Microscopy, Electron
chemical_list: D001786:Blood Glucose; D007328:Insulin; D007329:Insulin Antagonists; D007375:Interleukin-1; D005947:Glucose