3H-azidopine photoaffinity labeling of high molecular weight proteins in chloroquine resistant falciparum malaria.
Using 3H-azidopine, we have succeeded in labeling proteins from chloroquine resistant (CR) human falciparum malaria parasites in the molecular weight range of 155-170 kd. Vinblastine does not compete, but azidopine blocks the labeling using 3H-azidopine. Relatively little or no labeling of the 155-170 kd protein is seen in the chloroquine sensitive strain using 3H-azidopine. Further competition can be seen with nicardipine and reserpine (71%) respectively and verapamil (61%), chloroquine (48%), quinacrine (56%), trifluoperazine (32%) and chlorpromazine (33%). We speculate that this may be the glycoprotein responsible for the resistance to chloroquine in falciparum malaria.
Ye
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ZG
Department of Pharmacology/Toxicology, West Virginia University Health Sciences Center North, Morgantown 26506.
Van Dyke
K
K
Spearman
T
T
Safa
A R
AR
D000345:Affinity Labels; D000818:Animals; D001386:Azides / Q000378:metabolism*; D001667:Binding, Competitive; D002738:Chloroquine / Q000378:metabolism / Q000494:pharmacology*; D002746:Chlorpromazine / Q000378:metabolism; D004095:Dihydropyridines / Q000378:metabolism*; D004351:Drug Resistance; D006023:Glycoproteins / Q000378:metabolism; D008970:Molecular Weight; D009529:Nicardipine / Q000378:metabolism; D010777:Photochemistry; D010963:Plasmodium falciparum / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D011796:Quinacrine / Q000378:metabolism; D012110:Reserpine / Q000378:metabolism; D014268:Trifluoperazine / Q000378:metabolism; D014700:Verapamil / Q000378:metabolism
D000345:Affinity Labels; D001386:Azides; D004095:Dihydropyridines; D006023:Glycoproteins; D011506:Proteins; D014268:Trifluoperazine; C041130:azidopine; D002738:Chloroquine; D012110:Reserpine; D014700:Verapamil; D009529:Nicardipine; D011796:Quinacrine; D002746:Chlorpromazine