title: Chromobindin A, a Ca2+- and ATP-dependent chromaffin granule-binding protein, is found in a variety of tissues and in yeast.
abstract: Chromobindin A is a ring shaped, multisubunit protein which exhibits Ca2+ and ATP-dependent binding to chromaffin granule membranes. Here we report biochemical and immunochemical evidence for the presence of chromobindin A in a surprisingly broad range of tissues: The protein is abundant in bovine skeletal muscle, pancreas, adrenal cortex and in brain gray and white matter; it is present in low quantities in the parotid, intestine and spleen and is undetectable in lung. Interestingly, chromobindin A was also detected in extracts of yeast. Electron micrographs of the yeast protein reveal a morphology virtually identical to the mammalian protein. These results suggest that chromobindin A is an important protein in a wide variety of cell types and that it has been highly conserved through evolution.
authors.affiliation: Department of Pharmacology, University of Virginia School of Medicine, Charlottesville 22908.
authors.forename: W H
authors.initials: WH
authors.lastname: Martin
authors.forename: E
authors.initials: E
authors.lastname: Fromer
authors.forename: C E
authors.initials: CE
authors.lastname: Creutz
mesh_terms: D000818:Animals; D017302:Annexins; D002352:Carrier Proteins / Q000302:isolation & purification* / Q000378:metabolism; D002417:Cattle; D002837:Chromaffin Granules / Q000378:metabolism*; D002838:Chromaffin System / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D007425:Intracellular Membranes / Q000378:metabolism; D008565:Membrane Proteins / Q000302:isolation & purification* / Q000378:metabolism; D008854:Microscopy, Electron; D008970:Molecular Weight; D009928:Organ Specificity; D012441:Saccharomyces cerevisiae / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D017302:Annexins; D002352:Carrier Proteins; D008565:Membrane Proteins