Diagnostic accuracy of the immunocytochemical study of body fluids.
The diagnostic accuracy of the immunocytochemical characterization of body fluids was evaluated in 100 specimens (35 pleural, 40 peritoneal, 7 pericardial and 18 cerebrospinal [CSF] fluids) in comparison with routine morphologic examination. The immunochemical markers used for all specimens were common-leukocyte antigen, epithelial membrane antigen, epithelial keratin and desmin. Additional immunocytochemical studies for neurofilaments, glial fibrillary acidic protein, vimentin and melanoma-associated antigen were performed on the CSF specimens. The study confirmed the accuracy of the immunocytochemical characterization of cells in body fluids using a panel of immunocytochemical stains. These methods are recommended as an adjunct to improve the accuracy of the cytologic diagnosis of body fluids, especially in cases with diagnostically difficult morphologic features.
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Division of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905.
Ziesmer
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Wong
Y C
YC
Yam
L T
LT
D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis; D001202:Ascitic Fluid / Q000166:cytology / Q000276:immunology; D001826:Body Fluids / Q000166:cytology*; D002555:Cerebrospinal Fluid / Q000166:cytology / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry / Q000379:methods*; D007159:Immunologic Tests; D009369:Neoplasms / Q000175:diagnosis* / Q000276:immunology; D010496:Pericardium / Q000166:cytology / Q000276:immunology; D010994:Pleura / Q000166:cytology
D000911:Antibodies, Monoclonal; D000941:Antigens