title: Identification and genetic analysis of mkaA--a gene of the Salmonella typhimurium virulence plasmid necessary for intracellular growth.
abstract: Salmonella typhimurium, like many other Salmonella serovars, harbours a large plasmid required for mouse virulence and growth of bacteria in host cells. The nature of one virulence-abolishing Tn5 insertion (zzx-2556::Tn5) in the plasmid was characterized. A 3.7 kb insert harbouring this region was cloned in pBR325. Plasmid-directed protein synthesis in minicells indicated that the transposon had eliminated the expression of a 70 kDa protein encoded by the virulence plasmid. Nucleotide sequence analysis of the corresponding wild type DNA region showed a 1773 bp open reading frame (mkaA), encoding a protein of 591 amino acid residues and a predicted molecular mass of 60.6 kDa; zzx-2556::Tn5 was situated within mkaA.
authors.affiliation: Molecular Biology Unit, National Public Health Institute, Helsinki, Finland.
authors.forename: S
authors.initials: S
authors.lastname: Taira
authors.forename: M
authors.initials: M
authors.lastname: Rhen
mesh_terms: D001483:Base Sequence; D005798:Genes, Bacterial; D010957:Plasmids / Q000235:genetics*; D015183:Restriction Mapping; D012486:Salmonella typhimurium / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics