Deinococcus radiodurans DNA increases the radiation resistance of Escherichia coli.
A genomic DNA library of Deinococcus radiodurans DNA has been prepared using the plasmid vector pBR322. The recombinant plasmid was used to transform a more radiation-sensitive organism, Escherichia coli RR1. Following selection of transformed organisms by their ability to grow on ampicillin, radiation-resistant organisms were selected by irradiation with 137Cs gamma radiation. Increased radiation resistance correlates with the presence of a 3-kb fragment of DNA in these cells which is derived from D. radiodurans.
Dalrymple
G V
GV
Nuclear Medicine Service, John L. McClellan Memorial Veterans Hospital, Little Rock, Arkansas.
Lynch
T J
TJ
Hardin
J W
JW
D004926:Escherichia coli / Q000235:genetics*; D006094:Gram-Positive Bacteria / Q000235:genetics*; D011836:Radiation Tolerance / Q000235:genetics*; D014169:Transformation, Bacterial / Q000235:genetics*