Internalization of Shigella flexneri into HeLa cells occurs without an increase in cytosolic Ca2+ concentration.
Pathogenic Shigella flexneri invades epithelial cells through directed phagocytosis. The role of intracellular free calcium ([Ca2+]i) as a signal for this process was tested. No variation in the [Ca2+]i could be detected by using the fluorescent indicator fura 2 to measure the [Ca2+]i in HeLa cells during the invasion process. In addition, neither clamping nor maximal decreasing or increasing of the [Ca2+]i of HeLa cells had any effect on their susceptibility to invasion. These data demonstrate that [Ca2+]i is not a signal for S. flexneri directed phagocytosis in HeLa cells.
Clerc
P L
PL
Unité de Pathogénie Microbienne Moléculaire, Institut Pasteur, Paris, France.
Berthon
B
B
Claret
M
M
Sansonetti
P J
PJ
D002118:Calcium / Q000378:metabolism*; D003600:Cytosol / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D004848:Epithelium / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D006367:HeLa Cells / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D006801:Humans; D010587:Phagocytosis; D012763:Shigella flexneri / Q000276:immunology*
D002118:Calcium