Disassembly and reconstitution of yeast 60S ribosomal subunits.
Yeast 60S ribosomal subunits have been dissociated by reversible modification with dimethylmaleic anhydride. Treatment with 40 mumol reagent/ml releases 35% of the protein, producing core particles inactive in polyphenylalanine synthesis, which are totally or highly deficient in 17 different proteins. This preparation of residual particles recovers 45% of the original activity upon incubation with the released proteins. The reconstituted particles can be isolated by centrifugation without loss of activity, having the protein composition of the original subunits.
Nieto
M A
MA
Centro de Biología Molecular, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
Hernández
F
F
Palacián
E
E
D055598:Chemical Phenomena; D002621:Chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000302:isolation & purification / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008299:Maleic Anhydrides; D012331:RNA, Fungal / Q000032:analysis; D012335:RNA, Ribosomal / Q000032:analysis; D012269:Ribosomal Proteins / Q000302:isolation & purification* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000032:analysis* / Q000378:metabolism
D005656:Fungal Proteins; D008299:Maleic Anhydrides; D012331:RNA, Fungal; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; C007474:2,3-dimethylmaleic anhydride