title: [Enzyme immunoassay for thromboxane B2].
abstract: A sensitive and specific enzyme immunoassay for TXB2, the stable degradation product of thromboxane A2, was developed, in which the hapten molecule was labeled with pure peroxidase. After competitive binding to antibody between enzyme-labeled and free TXB2, the immunoreactive product was precipitated by double antibody technique, and the enzyme activity of the precipitate was determined spectrophotometrically. The procedures allowed a determination of 3-200 pg TXB2/tube (0.081 to 5.4 nmol/l).
authors.forename: C
authors.initials: C
authors.lastname: Giessler
authors.forename: M
authors.initials: M
authors.lastname: Panse
authors.forename: P
authors.initials: P
authors.lastname: Mentz
authors.forename: G
authors.initials: G
authors.lastname: Hellthaler
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D006168:Guinea Pigs; D007124:Immunoenzyme Techniques; D011237:Predictive Value of Tests; D013053:Spectrophotometry; D013929:Thromboxane B2 / Q000032:analysis*
chemical_list: D013929:Thromboxane B2
vernacular_title: Enzymimmunoassay für Thromboxan B2.