[Immunoenzyme analysis of adenovirus hexon. Determination of antibodies from hyperimmunized chickens].
We have elaborated three systems of enzyme-linked immunosorbent assay (ELISA) for detection of chicken IgG antibodies specific for hexon antigens of three immunologically distinct adenovirus groups: those of mammalian adenoviruses (Mastadenovira), typical avian adenoviruses (Aviadenovira) and of egg-drop syndrome-76 (EDS-76) virus. In each system the antibodies against respective hexons were specifically detected. In mammalian adenovirus hexons the ELISA detects primarily the type-specific (epsilon) and genus-specific (alpha) antigenic determinants. The time course of anti-hexon antibodies content was followed during immunization. The level of anti-hexon antibodies in egg yolk reflects adequately their content in blood serum. The technique is suitable for serological diagnosis of chicken adenoviral infections as well as for characterization of egg-yolk antibodies obtained by preparative hyperimmunization of hens.
Khil'ko
S N
SN
Kirasova
M A
MA
Piker
S D
SD
Osidze
S D
SD
Fomina
N V
NV
Burgasova
M P
MP
Tikhonenko
T I
TI
D000256:Adenoviridae / Q000235:genetics* / Q000276:immunology; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D002645:Chickens; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D007114:Immunization; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000302:isolation & purification
D000914:Antibodies, Viral; D036022:Capsid Proteins; D007074:Immunoglobulin G; C018315:hexon capsid protein, Adenovirus
Immunfermentnyĭ analiz geksona adenovirusov. Opredelenie antitel ot giperimmunizirovannykh kur.