A plasmid carrying mucA and mucB genes from pKM101 in Haemophilus influenzae and Escherichia coli.
The plasmid pMucAMucB, constructed from the Haemophilus influenzae vector pDM2, and a similar plasmid, constructed from pBR322, increased the survival after UV irradiation of Escherichia coli AB1157 with the umu-36 mutation and also caused UV-induced mutation in the E. coli strain. In H. influenzae, pMucAMucB caused a small but reproducible increase in survival after UV irradiation in wild-type cells and in a rec-1 mutant, but there was no increase in spontaneous mutation in the wild type or in the rec-1 mutant and no UV-induced mutation.
Spikes
D
D
Biology Department, Brookhaven National Laboratory, Upton, New York 11973.
Setlow
J K
JK
D004307:Dose-Response Relationship, Radiation; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D006193:Haemophilus influenzae / Q000235:genetics*; D009154:Mutation; D010957:Plasmids; D014466:Ultraviolet Rays