[Magnetic immunoenzyme analysis of Yersinia pestis antigens].
The detection of Y. pestis cells in magnetic enzyme immunoassay is carried out with the use of magnetic polyacrylamide microgranules. In the assay system for the determination of the antigen commercial Y. pestis antigens, peroxidase-labeled antibodies, the substrate mixture consisting of sodium salt of 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid and H2O2 in citrate-phosphate buffer solution, pH 4.5, are used. The sensitivity of the method is 5 X 10(4) microbial bodies per ml.
Klimova
I M
IM
Efremenko
V I
VI
Pushkar'
V G
VG
D000327:Adsorption; D000942:Antigens, Bacterial / Q000032:analysis*; D005069:Evaluation Studies as Topic; D007124:Immunoenzyme Techniques / Q000592:standards; D007164:Immunosorbents; D008280:Magnetics / Q000379:methods* / Q000592:standards; D012995:Solubility; D015010:Yersinia pestis / Q000276:immunology*
D000942:Antigens, Bacterial; D007164:Immunosorbents
Magnitnyĭ immunofermentnyĭ analiz antigenov Yersinia pestis.