title: Expression of human insulin and hybrid insulin genes in pancreatic beta cells of transgenic mice.
abstract: Transgenic mice which specifically express the human insulin gene in the pancreatic beta cells were obtained by microinjection of DNA into fertilized one-cell embryos. The elements regulating in vivo the beta-cell specificity of the insulin gene, located within a few hundred base pairs upstream to the start site of transcription, were used to construct hybrid genes expressed in pancreatic beta cells. Expression of SV40-T large antigen resulted in beta-cell proliferation and beta-cell tumors, while major histocompatibility, or interferon-gamma molecules induced diabetes mellitus in transgenic mice.
authors.affiliation: Institut Jacques-Monod, INSERM, U. 257, CNRS, Paris, France.
authors.forename: D
authors.initials: D
authors.lastname: Bucchini
authors.forename: L
authors.initials: L
authors.lastname: Deltour
authors.forename: J
authors.initials: J
authors.lastname: Jami
mesh_terms: D000818:Animals; D015870:Gene Expression; D006801:Humans; D007328:Insulin / Q000096:biosynthesis / Q000235:genetics*; D007515:Islets of Langerhans / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic
chemical_list: D007328:Insulin