Processing and secretion of barley (1-3,1-4)-beta-glucanase in yeast.
DNA segments encoding signal peptides from mouse alpha-amylase, yeast acid phosphatase, and yeast invertase were fused in frame to a barley (1-3,1-4)-beta-glucanase cDNA gene and expressed in yeast cells under the control of the phosphoglycerate kinase gene promoter. Pure beta-glucanase is obtained by gel filtration of concentrated yeast cell supernatant. It was shown that the glucanase pre-protein was specifically processed and the mature protein efficiently secreted when the yeast invertase signal sequence directed secretion.
Olsen
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Department of Physiology, Carlsberg Laboratory, Copenhagen Valby.
Thomsen
K K
KK
D000135:Acid Phosphatase / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005796:Genes; D005800:Genes, Fungal; D006026:Glycoside Hydrolases / Q000235:genetics* / Q000378:metabolism; D001467:Hordeum / Q000201:enzymology / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010944:Plants / Q000201:enzymology / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011487:Protein Conformation; D021382:Protein Sorting Signals / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D000516:alpha-Amylases / Q000235:genetics; D043324:beta-Fructofuranosidase
D015345:Oligonucleotide Probes; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D000135:Acid Phosphatase; D006026:Glycoside Hydrolases; D000516:alpha-Amylases; D043324:beta-Fructofuranosidase; C028020:licheninase