Soluble proteins from Schistosoma mansoni and japonicum: a comparative biochemical and immunological analysis.
1. Soluble proteins were recovered from male Schistosoma mansoni after homogenization in Tris-HCl buffer containing 0.6 M KCl and 1.0% Triton X-100 followed by preparative electrophoresis on SDS-gel. 2. Polyclonal antibodies produced in mice against the soluble fraction were used in comparative analysis of S. mansoni and S. japonicum using immunoblots and immunoprecipitation of in vitro translated polypeptides. 3. Small molecular weight polypeptide (20-22 kdalton), identified by infected mouse serum (IMS) on immunoblots, was predominant in females and was not cross-reactive with heterologous IMS. 4. A 41-43 kdalton polypeptide which appeared as a doublet on immunoblots performed with polyclonal antiserum 4M, was predominant in males of both species although the polypeptides of S. mansoni showed slower electrophoretic mobility, and therefore the larger size (43 kdalton), than that of S. japonicum. 5. Comparison of fluorograms of the immunoprecipitates of in vitro translated polypeptides indicated that IMS of S. mansoni precipitated two, 30 and 94 kdalton, polypeptides while the IMS of S. japonicum identified at 72 kdalton polypeptide. Antisera 1M, 2M and 4M also showed similarities and differences in polypeptides of in vitro translation products of the two species of Schistosoma.
Abbas
M K
MK
Department of Medicine, University of Iowa, Iowa City 52242.
Foster
L A
LA
Cain
G D
GD
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D066298:In Vitro Techniques; D008297:Male; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis*; D012333:RNA, Messenger / Q000302:isolation & purification; D012549:Schistosoma japonicum / Q000032:analysis*; D012550:Schistosoma mansoni / Q000032:analysis*; D013045:Species Specificity
D011506:Proteins; D012333:RNA, Messenger