title: Class-specific and polyvalent enzyme-linked immunosorbent assays for detection of antibodies to Borrelia burgdorferi in equids.
abstract: Class-specific and polyvalent ELISA were developed to detect IgM antibody or total immunoglobulins to Borrelia burgdorferi in equine sera. Analyses of 122 serum specimens, collected during 1985 from horses and ponies in tick-infested areas of Connecticut, revealed IgM antibody in 41 (34%) samples; titration end points ranged from 1:160 to 1:2,560. In polyvalent ELISA, 73 (16%) of 454 serum specimens contained IgM and/or IgG antibody. Seropositivity was highest (32%) for blood samples collected during May. Both ELISA procedures had comparable sensitivities.
authors.affiliation: Department of Entomology, Connecticut Agricultural Experiment Station, New Haven 06504.
authors.forename: L A
authors.initials: LA
authors.lastname: Magnarelli
authors.forename: J F
authors.initials: JF
authors.lastname: Anderson
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D015748:Borrelia burgdorferi Group / Q000276:immunology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D006734:Horse Diseases / Q000175:diagnosis*; D006736:Horses; D007075:Immunoglobulin M / Q000032:analysis; D007136:Immunoglobulins / Q000032:analysis; D008193:Lyme Disease / Q000175:diagnosis / Q000662:veterinary*; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D012621:Seasons
chemical_list: D000907:Antibodies, Bacterial; D007075:Immunoglobulin M; D007136:Immunoglobulins