Inhibition of hexose transport by glucose in a glucose-6-phosphate isomerase mutant of Saccharomyces cerevisiae.
The rate of hexose transport was approximately 60% lower for both the high- and the low-affinity components of hexose uptake when a glucose-6-phosphate isomerase mutant of Saccharomyces cerevisiae was preincubated with glucose, as compared with preincubation with water. Similarly the Jmax value of the high-affinity system of the mutant was 25-35% of the corresponding Jmax value for normal cells incubated with glucose. Accumulation of glucose 6-phosphate or of some other metabolite, such as fructose 6-phosphate or trehalose, may be responsible for this striking inhibition.
Alonso
A
A
Department of Genetics, National Center for Scientific Research, Havana, Cuba.
Pascual
C
C
Romay
C
C
Herrera
L
L
Kotyk
A
A
D001693:Biological Transport, Active / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics / Q000378:metabolism; D006601:Hexoses / Q000493:pharmacokinetics*; D007700:Kinetics; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*
D006601:Hexoses; D005956:Glucose-6-Phosphate Isomerase; D005947:Glucose