title: [The relation of glutathione reductase and diaphorase activity of glutathione reductase from Saccharomyces cerevisiae].
abstract: Glutathione reductase from S. cerevisiae (EC 1.6.4.2) catalyzes the NADPH oxidation by glutathione in accordance with a "ping-pong" scheme. The catalytic constant kcat) is 240 s-1 (pH 7.0, 25 degrees C); kcat for the diaphorase reaction is 4-5 s-1. The enzyme activity does not change markedly at pH 5.5-8.0. At pH less than or equal to 7.0, NADP+ acts as a competitive inhibitor towards NADPH and as a noncompetitive inhibitor towards glutathione. NADP+ increases the diaphorase activity of the enzyme. The maximal activity is observed, when the NADP+/NADPH ratio exceeds 100. At pH 8.0, NADP+ acts as a mixed type inhibitor during the reduction of glutathione. High concentrations of NADP+ also inhibit the diaphorase activity due to the reoxidation of the reduced enzyme by NADP+ at pH 8.0. The redox potential of glutathione reductase calculated from the inhibition data is--306 mV (pH 8.0). Glutathione reductase reduces quinoidal compounds in an one-electron way. The hyperbolic dependence of the logarithm of the oxidation constant on the one electron reduction potential of quinone is observed. It is assumed that quinones oxidize the equilibtium fraction of the two-electron reduced enzyme containing reduced FAD.
authors.forename: N K
authors.initials: NK
authors.lastname: Chenas
authors.forename: G A
authors.initials: GA
authors.lastname: Rakauskene
authors.forename: Iu Iu
authors.initials: IuIu
authors.lastname: Kulis
mesh_terms: D008058:Dihydrolipoamide Dehydrogenase / Q000378:metabolism*; D004579:Electron Transport; D005980:Glutathione Reductase / Q000378:metabolism*; D009249:NADP / Q000037:antagonists & inhibitors / Q000378:metabolism; D010084:Oxidation-Reduction; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
chemical_list: D009249:NADP; D008058:Dihydrolipoamide Dehydrogenase; D005980:Glutathione Reductase
vernacular_title: Sootnosheniia mezhdu glutationreduktaznoĭ i diaforaznoĭ aktivnost'iu glutationreductazy iz Saccharomyces cerevisiae.