Conversion of monofunctional DNA adducts of cis-diamminedichloroplatinum (II) to bifunctional lesions. Effect on the in vitro replication of single-stranded DNA by Escherichia coli DNA polymerase I and eukaryotic DNA polymerases alpha.
Reaction of cis-diamminedichloroplatinum (II) with single-stranded M13 phage DNA in vitro produced monofunctional platinum-DNA adducts on guanine and bifunctional lesions with either two guanine bases (GG) or one adenine and one guanine (AG). When DNA containing a majority of monofunctional platinum-DNA lesions was dialyzed against 10 mM NaCIO4 at 37 degrees C, conversion of monoadducts to bifunctional lesions was observed. We examined the effect of post-treatment formation of bifunctional lesions on DNA synthesis by Escherichia coli DNA polymerase I and highly purified eukaryotic DNA polymerase alpha from Drosophila melanogaster and calf thymus. Arrest sites on the platinated template were determined by polyacrylamide gel electrophoresis. Monofunctional lesions did not appear to block DNA synthesis. Inhibition of replication increased as bifunctional platinum-DNA lesions formed during post-treatment incubation; GG adducts inhibited replication more than AG. These results suggest that bifunctional GG platinum-DNA adducts may be the major toxic damage of cisplatin.
Hoffmann
J S
JS
Laboratoire de Pharmacologie et de Toxicologie Fondamentales, Centre National de la Recherche Scientifique, Toulouse, France.
Johnson
N P
NP
Villani
G
G
D000818:Animals; D002417:Cattle; D002945:Cisplatin / Q000378:metabolism / Q000633:toxicity; D004256:DNA Polymerase I / Q000235:genetics*; D004257:DNA Polymerase II / Q000235:genetics*; D004261:DNA Replication / Q000187:drug effects*; D004269:DNA, Bacterial / Q000187:drug effects*; D004277:DNA, Single-Stranded / Q000187:drug effects*; D004330:Drosophila / Q000201:enzymology / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000201:enzymology; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005785:Gene Conversion / Q000187:drug effects; D013698:Templates, Genetic
D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D004256:DNA Polymerase I; D004257:DNA Polymerase II; D002945:Cisplatin