title: Presence of endothelin-1 in porcine spinal cord: isolation and sequence determination.
abstract: We investigated the molecular forms of endothelin (ET) related peptides in porcine spinal cord by high performance liquid chromatography coupled with radioimmunoassays using three antisera raised against ET-1 and C-terminal fragments of ET-1 and big ET-1. ET-1 and its oxidized form were isolated as major immunoreactive peptides and sequenced. Furthermore, immunoreactivities like ET-3 and big ET-1(22-39) (contents: less than 8% and less than 1% of ET-1, respectively) were detected based on their chromatographic retention times and characteristics of immunoreactivity to the antisera. Big ET-1 was only scarcely detected. Immunohistochemical study showed the presence of ET-1-like immunoreactivity in motoneurons, dorsal horn neurons and dot- and fiber-like structures in the dorsal horn of lumbar spinal cord. These results indicate that ET-1 is present not only in endothelial cells but also in spinal cord, and that big ET-1 is converted into ET-1 in spinal cord by specific processing between Trp21-Val22. The data also indicate that ET-1 may act as a neuropeptide in the central nervous system.
authors.affiliation: Department of Biochemistry, University of Tsukuba, Ibaraki, Japan.
authors.forename: O
authors.initials: O
authors.lastname: Shinmi
authors.forename: S
authors.initials: S
authors.lastname: Kimura
authors.forename: T
authors.initials: T
authors.lastname: Yoshizawa
authors.forename: T
authors.initials: T
authors.lastname: Sawamura
authors.forename: Y
authors.initials: Y
authors.lastname: Uchiyama
authors.forename: Y
authors.initials: Y
authors.lastname: Sugita
authors.forename: I
authors.initials: I
authors.lastname: Kanazawa
authors.forename: M
authors.initials: M
authors.lastname: Yanagisawa
authors.forename: K
authors.initials: K
authors.lastname: Goto
authors.forename: T
authors.initials: T
authors.lastname: Masaki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D016232:Endothelins; D004730:Endothelium, Vascular / Q000032:analysis*; D008297:Male; D008969:Molecular Sequence Data; D009046:Motor Neurons / Q000032:analysis; D010446:Peptide Fragments / Q000302:isolation & purification; D010455:Peptides / Q000302:isolation & purification*; D011863:Radioimmunoassay; D013116:Spinal Cord / Q000032:analysis*; D013552:Swine; D013556:Swine, Miniature; D014662:Vasoconstrictor Agents / Q000302:isolation & purification*
chemical_list: D016232:Endothelins; D010446:Peptide Fragments; D010455:Peptides; D014662:Vasoconstrictor Agents