Membrane filtration differentiation of E. coli from coliforms in the examination of water.
A membrane filter-Endo agar method for enumerating Escherichia coli as distinct from other coliforms in drinking water was developed. Membranes containing coliform colonies are transferred to nutrient agar containing 4-methyl umbelliferyl-beta-D-glucuronide (MUG) and incubated at 35 degrees C for 4 h. The MUG is hydrolyzed by the glucuronidase of E. coli and the fluorogenic product is visualized. The method recovered 98% of E. coli without false positives and is proposed as an additional test in routine water examination for the detection of pollution.
Mates
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A
Public Health Laboratory, Haifa, Israel.
Shaffer
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M
D015169:Colony Count, Microbial; D003470:Culture Media; D004755:Enterobacteriaceae / Q000254:growth & development / Q000302:isolation & purification*; D004926:Escherichia coli / Q000254:growth & development / Q000302:isolation & purification*; D005374:Filtration; D014871:Water Microbiology; D014881:Water Supply
D003470:Culture Media