Modulation of mouse renin gene expression by dietary sodium chloride intake in one-gene, two-gene and transgenic animals.
We have studied the effect of dietary NaCl loading on renin gene expression in one-gene, two-gene and transgenic mouse strains. By Northern blotting, we found an approximate twofold reduction in renin messenger (m) RNA in the kidneys of high-NaCl-treated compared with low-NaCl-treated animals. Using an RNase-protection assay designed to discriminate between the different renin gene transcripts, we have shown that renin mRNAs derived from the Ren-1C gene of one-gene strains and the Ren-1D and Ren-2 genes of two-gene animals are all NaCl-responsive. Renin mRNA derived from a 19 kilobase Ren-1D transgene is also NaCl-responsive.
Miller
C C
CC
ICI/University Joint Laboratory, University of Leicester, UK.
Samani
N J
NJ
Carter
A T
AT
Brooks
J I
JI
Brammar
W J
WJ
D000818:Animals; D015152:Blotting, Northern; D005786:Gene Expression Regulation; D007668:Kidney / Q000378:metabolism; D007797:Laminin / Q000096:biosynthesis; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D012333:RNA, Messenger / Q000096:biosynthesis; D012083:Renin / Q000096:biosynthesis*; D012982:Sodium, Dietary / Q000494:pharmacology*
D007797:Laminin; D012333:RNA, Messenger; D012982:Sodium, Dietary; D012083:Renin