Expression of c-raf-1 and A-raf-1 during regeneration of rat liver following surgical partial hepatectomy.
The major objective of this study was to investigate the expression of members of the raf family of proto-oncogenes during rat liver regeneration. The steady-state level of expression of both c-raf-1 and A-raf-1 increased three- to fivefold 18-24 h following partial hepatectomy, and it returned to basal levels by 72 h. Expression of c-myc and Ha-ras mRNA was increased at 3 and 18-24 h, respectively, confirming previous reports. Increased steady-state levels of c-raf-1, A-raf-1, and Ha-ras mRNA were also detected in hepatocytes isolated from rat liver 24 h after partial hepatectomy. Thus, elevated expression of the raf genes closely correlated with that of Ha-ras, beginning at 12 h and reaching maximal levels during the first peak of DNA synthesis following partial hepatectomy.
Silverman
J A
JA
Department of Pharmacology and Toxicology, Dartmouth Medical School, Hanover, New Hampshire.
Zurlo
J
J
Watson
M A
MA
Yager
J D
JD
D000818:Animals; D015152:Blotting, Northern; D005260:Female; D006498:Hepatectomy; D008115:Liver Regeneration; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics; D019908:Proto-Oncogene Proteins c-raf; D016283:Proto-Oncogene Proteins p21(ras); D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats
D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D019908:Proto-Oncogene Proteins c-raf; D016283:Proto-Oncogene Proteins p21(ras)