title: Purification and amino acid sequencing of naturally occurring N-formyl-methionyl oligopeptides from Escherichia coli.
abstract: A novel purification procedure for N-formyl methionyl oligopeptides from bacterial culture supernatants has been developed. C-terminal carboxypeptidase microsequencing of purified peptides from E. coli supernatants enabled identification of 6 naturally occurring formyl-oligopeptides.
authors.affiliation: Wellcome Medical Research Institute, University of Otago Medical School, Dunedin, New Zealand.
authors.forename: M F
authors.initials: MF
authors.lastname: Broom
authors.forename: D M
authors.initials: DM
authors.lastname: Mellor
authors.forename: V S
authors.initials: VS
authors.lastname: Chadwick
mesh_terms: D000595:Amino Acid Sequence; D002268:Carboxypeptidases; D004926:Escherichia coli / Q000378:metabolism*; D008715:Methionine / Q000031:analogs & derivatives; D008969:Molecular Sequence Data; D009239:N-Formylmethionine; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000302:isolation & purification; D009842:Oligopeptides / Q000302:isolation & purification*
chemical_list: D009842:Oligopeptides; D009239:N-Formylmethionine; D009240:N-Formylmethionine Leucyl-Phenylalanine; D008715:Methionine; D002268:Carboxypeptidases; C022633:serine carboxypeptidase