[Fluorescent immunoanalysis. Synthesis, spectro-fluorescent and immunologic properties of conjugates of coproporphyrin I with antibodies].
The synthesis of protein conjugates with the new high-efficient fluorescent labile coproporphyrin-I was optimized. A number of conjugates of monoclonal antibodies with different coproporphyrin-I content were synthesized, and their spectral properties were studied in water and micellar solutions, i.e. adsorption, excitation and emission spectra, fluorescence quantum yields, fluorescence pH-dependences. The binding constants of coproporphyrin-I and its protein conjugates with serum albumin were determined. The antibodies labelled with coproporphyrin-I retain the functional activity and photochemically stable in water solutions. The sensitivity of fluorometric detection of coproporphyrin-I and its conjugates with proteins is more than 10 times greater than in case of FITC.
Papkovskiĭ
D B
DB
Savitskiĭ
A P
AP
Ugarova
N N
NN
Berezin
I V
IV
Ponomarev
G V
GV
D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D002853:Chromatography, Liquid; D003306:Coproporphyrins / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000276:immunology* / Q000302:isolation & purification; D010777:Photochemistry; D011166:Porphyrins / Q000276:immunology*; D012709:Serum Albumin / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013050:Spectrometry, Fluorescence
D000911:Antibodies, Monoclonal; D003306:Coproporphyrins; D007074:Immunoglobulin G; D011166:Porphyrins; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; C031467:coproporphyrin I
Fluorestsentnĭ immunoanaliz. Sintez, spektral'no-fluorestsentnye i immunologicheskie svoĭstva kon''iugatov koproporfirina I s antitelami