Elongation factor EF-1 alpha gene dosage alters translational fidelity in Saccharomyces cerevisiae.
Changes in the dosage of genes encoding elongation factor EF-1 alpha were shown to cause parallel changes in the misreading of nonsense codons. Higher amounts of EF-1 alpha were correlated with increased nonsense suppression, suggesting that the level of EF-1 alpha is critically involved in translational fidelity.
Song
J M
JM
Department of Biological Sciences, University of Illinois, Chicago 60680.
Picologlou
S
S
Grant
C M
CM
Firoozan
M
M
Tuite
M F
MF
Liebman
S
S
D003001:Cloning, Molecular; D005784:Gene Amplification / Q000502:physiology; D010303:Paromomycin / Q000494:pharmacology; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000096:biosynthesis / Q000235:genetics*; D014176:Protein Biosynthesis / Q000502:physiology*; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013489:Suppression, Genetic / Q000502:physiology
D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors; D010303:Paromomycin