title: Method for improved extraction of DNA from Nocardia asteroides.
abstract: In a variation of standard DNA extraction methods, Nocardia asteroides was repeatedly exposed to sodium dodecyl sulfate at 60 degrees C for 30 min; each extraction was followed by centrifugation, removal of the nucleic acid-rich supernatant, and suspension of the cell pellet in fresh sodium dodecyl sulfate. The pooled supernatants contained a substantially higher amount of DNA than the first supernatant alone. The possible implications of this procedure on the development of DNA probes are discussed.
authors.affiliation: Diagnostic Hybrids, Inc., Athens, Ohio 45701.
authors.forename: M J
authors.initials: MJ
authors.lastname: Loeffelholz
authors.forename: D R
authors.initials: DR
authors.lastname: Scholl
mesh_terms: D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D009616:Nocardia asteroides / Q000235:genetics*; D012967:Sodium Dodecyl Sulfate
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial; D012967:Sodium Dodecyl Sulfate