title: A scanning calorimetric study of the thermal denaturation of the lysozyme of phage T4 and the Arg 96----His mutant form thereof.
abstract: High-sensitivity scanning calorimetry has been employed to study the reversible thermal unfolding of the lysozyme of T4 bacteriophage and of its mutant form Arg 96----His in the pH range 1.80-2.84. The values for t1/2, the temperature of half-denaturation, in degrees Celsius and for the enthalpy of unfolding in kilocalories per mole are given by (standard deviations in parentheses) wild type t1/2 = 9.63 + 14.41 pH (+/- 0.58) delta Hcal = 5.97 + 2.33t (+/- 4.20) mutant form t1/2 = -19.84 + 21.31 pH (+/- 0.51) delta Hcal = -8.58 + 2.66t (+/- 4.48) At any temperature within the range -20 to 60 degrees C, the free energy of unfolding of the mutant form is more negative than that of the wild type by 3-5 kcal mol-1, indicating an apparent destabilization resulting from the arginine to histidine replacement. The ratio of the van't Hoff enthalpy to the calorimetric enthalpy deviates from unity, the value expected for a simple two-state process, by +/- 0.2 depending on the pH. It thus appears that the nature of the unfolding of T4 lysozyme varies with pH in unknown manner. This complication does not invalidate the values reported here for the temperature of half-completion of unfolding, the calorimetric enthalpy, the heat capacity change, or the free energy of unfolding.
authors.affiliation: Department of Chemistry, Yale University, New Haven, Connecticut 06520.
authors.forename: S
authors.initials: S
authors.lastname: Kitamura
authors.forename: J M
authors.initials: JM
authors.lastname: Sturtevant
mesh_terms: D001120:Arginine; D002152:Calorimetry, Differential Scanning; D004926:Escherichia coli / Q000201:enzymology*; D006639:Histidine; D008433:Mathematics; D009113:Muramidase / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D011489:Protein Denaturation; D013604:T-Phages / Q000201:enzymology*; D013816:Thermodynamics
chemical_list: D006639:Histidine; D001120:Arginine; D009113:Muramidase