Characterization of a plasmid coding for resistance to broad-spectrum cephalosporins in Salmonella typhimurium.
Five strains of Salmonella typhimurium isolated from faeces of infants hospitalized at Trousseau Hospital in Paris have been found to be resistant to third-generation cephalosporins. This resistance is by a transferable plasmid-mediated mechanism. The beta-lactamase gene which encodes for an enzyme similar to SHV-2 has been cloned into plasmid pBR322 and expressed in Escherichia coli RR1.
Garbarg-Chenon
A
A
Laboratory of Microbiology, Trousseau Hospital, Paris, France.
Vu Thien
H
H
Labia
R
R
Ben-Yaghlane
H
H
Godard
V
V
Deny
P
P
Bricout
F
F
Nicolas
J C
JC
D002439:Cefotaxime / Q000494:pharmacology; D002442:Ceftazidime / Q000494:pharmacology; D002511:Cephalosporins / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D006801:Humans; D007525:Isoelectric Focusing; D007700:Kinetics; D010400:Penicillin G / Q000494:pharmacology; D010641:Phenotype; D010957:Plasmids; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D014170:Transformation, Genetic
D002511:Cephalosporins; D004269:DNA, Bacterial; D002442:Ceftazidime; D002439:Cefotaxime; D010400:Penicillin G