title: Molecular biology of enzyme adaptations in higher eukaryotes.
abstract: Multicellular eukaryotes have evolved complex homeostatic mechanisms that buffer the majority of their cells from direct interaction with the external environment. Thus, in these organisms long-term adaptations are generally achieved by modulating the developmental profile and tissue specificity of gene expression. Nevertheless, a subset of eukaryotic genes are still involved in direct responses to environmental fluctuations. It is the adaptative responses in the expression of these genes that buffers many other genes from direct environmental effects. Both microevolutionary and macroevolutionary patterns of change in the structure and regulation of such genes are illustrated by the sequences encoding alpha-amylases. The molecular biology and evolution of alpha-amylases in Drosophila and other higher eukaryotes are presented. The amylase system illustrates the effects of both long-term and short-term natural selection, acting on both the structural and regulatory components of a gene--enzyme system. This system offers an opportunity for linking evolutionary genetics to molecular biology, and it allows us to explore the relationship between short-term microevolutionary changes and long-term adaptations.
authors.affiliation: Department of Biology, University of Ottawa, Ont., Canada.
authors.forename: D A
authors.initials: DA
authors.lastname: Hickey
authors.forename: B F
authors.initials: BF
authors.lastname: Benkel
authors.forename: C
authors.initials: C
authors.lastname: Magoulas
mesh_terms: D000220:Adaptation, Biological / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D004798:Enzymes / Q000235:genetics*; D005786:Gene Expression Regulation; D005809:Genes, Regulator / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D012641:Selection, Genetic; D000516:alpha-Amylases / Q000235:genetics
chemical_list: D004798:Enzymes; D000516:alpha-Amylases