Crystallization and preliminary X-ray studies of an aspartate aminotransferase mutant from Escherichia coli.
Mutant aspartate aminotransferase V39L (Val39 replaced by Leu) from Escherichia coli has been crystallized into a monoclinic cell from a polyethylene glycol solution (pH 7.5) by vapor diffusion. The space group and the unit cell dimensions have been determined using a precession camera, a CAD4 diffractometer and a Nicolet Xentronics area detector to be P2(1) with a = 86.8 A, b = 79.9 A, c = 89.4 A, beta = 118.74 degrees. The crystals diffract to better than 2.3 A and are suitable for X-ray structure analysis.
Jäger
J
J
Department of Structural Biology, University of Basel, Switzerland.
Köhler
E
E
Tucker
P
P
Sauder
U
U
Housley-Markovic
Z
Z
Fotheringham
I
I
Edwards
M
M
Hunter
M
M
Kirschner
K
K
Jansonius
J N
JN
D001219:Aspartate Aminotransferases; D003460:Crystallization; D004926:Escherichia coli / Q000201:enzymology*; D008958:Models, Molecular; D009154:Mutation; D011487:Protein Conformation; D014961:X-Ray Diffraction
D001219:Aspartate Aminotransferases