[In vivo effect of etoposide on the function of bone marrow-derived macrophages].
Proliferative and colony-forming abilities of bone marrow cells to M-CSF and the function of the bone marrow-derived macrophages were examined 2, 7, 14 and 21 days after intraperitoneal injection of etoposide. Only two days after administration of etoposide (50 mg/kg of body weight), the number of bone marrow cells per femur was significantly decreased, but completely recovered by day 7. In contrast, proliferative and colony-forming responses of the bone marrow cells to M-CSF was increased after 2 days, but there-after returned to the normal. The abilities of bone marrow-derived macrophages to adhere to plastics and to produce monokines (IL-1 and TNF) were the same as those of controls before and after treatment with etoposide. These data suggest that activation of macrophage progenitors in bone marrow may be useful in combination with chemotherapy for treatment of malignant diseases.
Nakanishi
M
M
Third Dept. of Internal Medicine, University of Tokushima School of Medicine.
Sone
S
S
Inamura
N
N
Mahendra
S S
SS
Shimizu
E
E
Ogura
T
T
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects*; D001854:Bone Marrow Cells; D002455:Cell Division / Q000187:drug effects; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000494:pharmacology; D005047:Etoposide / Q000494:pharmacology*; D007375:Interleukin-1 / Q000096:biosynthesis; D016173:Macrophage Colony-Stimulating Factor; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D003115:Colony-Stimulating Factors; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D005047:Etoposide; D016173:Macrophage Colony-Stimulating Factor