Isolation and characterization of a mucosal triacylglycerol pool undergoing hydrolysis.
Absorbed and processed mucosal neutral lipid has been shown to be composed of at least two pools of triacylglycerol. One is likely to subserve chylomicron formation, and the other appears to be transported from the intestine via a nonlymphatic route. In the present study, 50 +/- 5% of the mucosal lipid pellets was centrifuged at 75,000 g.min [low-speed pellet (LSP)]. Discontinuous sucrose density gradient centrifugation of LSP showed that 61 +/- 7% of the lipid banded at the 0.25-0.86 M sucrose interface. Neutral lipid analysis showed that this subfraction was only 58% triacylglycerol, suggesting it was undergoing hydrolysis. Active lipolytic activity in vitro was found on incubation. The lipase had an alkaline pH optimum (pH 8.5) and persisted despite pancreatic ductular diversion. Lipolysis in vivo in a LSP fraction was shown by infusing [14C]glyceryltrioleate for 3.5 h followed by [3H]glyceryltrioleate for 30 min. Discontinuous sucrose density centrifugation of the LSP followed by an analysis of the lipids at the 0.25-0.86 M sucrose interface showed that 14C-neutral lipids were only 70 +/- 6% triacylglycerol, whereas 3H-neutral lipids were 88 +/- 2% triacylglycerol. 3H entered LSP slowly compared with the floating lipid in the same centrifuge tube. These studies suggest both in vivo and in vitro mucosal lipolysis by a specific, alkaline-active lipase. The turnover rate of LSP is likely to be slow by comparison with neutral lipid floating to the top of the centrifuge tube.
Tipton
A D
AD
Department of Medicine, University of Tennessee, Memphis 38163.
Frase
S
S
Mansbach
C M
CM
D000818:Animals; D002250:Carbon Radioisotopes; D002784:Cholesterol / Q000378:metabolism; D004386:Duodenum / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000648:ultrastructure; D008066:Lipolysis; D008297:Male; D008854:Microscopy, Electron; D010183:Pancreatic Ducts / Q000502:physiology; D010743:Phospholipids / Q000378:metabolism; D011865:Radioisotope Dilution Technique; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D014280:Triglycerides / Q000378:metabolism*; D014304:Triolein / Q000378:metabolism*; D014316:Tritium
D002250:Carbon Radioisotopes; D010743:Phospholipids; D014280:Triglycerides; D014316:Tritium; D014304:Triolein; D002784:Cholesterol