title: Prorenin is sorted into the regulated secretory pathway independent of its processing to renin in mouse pituitary AtT-20 cells.
abstract: A native human prorenin and a mutant of its processing site (Arg-43 to Gln) were expressed in mouse pituitary AtT-20 cells which process prorenin to renin and have both regulated and constitutive secretory pathways. The native prorenin was processed to renin and secreted in a regulated manner. Although the mutant precursor was not processed, it was also secreted in a regulated manner. These results suggest that prorenin is sorted into the regulated pathway, stored in secretory granules and released by stimulus whether it is processed to renin or not.
authors.affiliation: Institute of Biological Sciences, University of Tsukuba, Ibaraki, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nakayama
authors.forename: M
authors.initials: M
authors.lastname: Nagahama
authors.forename: W S
authors.initials: WS
authors.lastname: Kim
authors.forename: K
authors.initials: K
authors.lastname: Hatsuzawa
authors.forename: K
authors.initials: K
authors.lastname: Hashiba
authors.forename: K
authors.initials: K
authors.lastname: Murakami
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001120:Arginine; D001483:Base Sequence; D002460:Cell Line; D004792:Enzyme Precursors / Q000235:genetics*; D005973:Glutamine; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D010911:Pituitary Neoplasms; D010957:Plasmids; D011499:Protein Processing, Post-Translational; D012083:Renin / Q000235:genetics*; D015183:Restriction Mapping
chemical_list: D004792:Enzyme Precursors; D005973:Glutamine; D001120:Arginine; D012083:Renin