Detection of ionizing radiations with the SOS Chromotest, a bacterial short-term test for genotoxic agents.
The effects of 3 types of ionizing radiation, gamma-rays, neutrons and accelerated alpha-particles, were examined using the SOS Chromotest, a bacterial colorimetric assay for genotoxic agents based on the measurement of the SOS response in Escherichia coli. The SOS Chromotest appeared to be a sensitive and simple assay to detect quantitatively these radiations as well as their biological effects. The range of adsorbed doses for which induction was observed was similar for the 3 types of radiation, the minimum inducing doses being in the order of 2.5-5 Gy. We discuss the possible use of these observations to study the molecular action of radiations and to compare their genotoxic effects with those of chemicals.
Quillardet
P
P
CNRS 040271, INSERM UA163, Institut Pasteur, Paris, France.
Frelat
G
G
Nguyen
V D
VD
Hofnung
M
M
D004260:DNA Repair; D004926:Escherichia coli / Q000235:genetics / Q000528:radiation effects*; D009152:Mutagenicity Tests; D013014:SOS Response, Genetics