title: The recB gene product is essential for exonuclease V-dependent DNA degradation in vivo.
abstract: The recB21 mutation abolishes the exonuclease activity of the RecBCD enzyme (exonuclease V) of Escherichia coli. This might be due to the polar effect of recB21 on expression of the recD gene, the product of which is an essential component of the RecBCD enzyme. To achieve synthesis of the recD gene product, the recD+ plasmid was introduced into the recB21 mutant. Degradation of the endogenous DNA damaged by gamma-rays and degradation of the DNA of a phage T4 gene 2 mutant were nevertheless abnormally small in this strain. Thus, the functional recB gene product is required for the degradative function of the RecBCD enzyme.
authors.affiliation: Institute Ruder Bosković, Zagreb, Croatia, Yugoslavia.
authors.forename: K
authors.initials: K
authors.lastname: Brcić-Kostić
authors.forename: I
authors.initials: I
authors.lastname: Stojiljković
authors.forename: E
authors.initials: E
authors.lastname: Salaj-Smic
authors.forename: Z
authors.initials: Z
authors.lastname: Trgovcević
mesh_terms: D004260:DNA Repair; D004269:DNA, Bacterial / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins; D043211:Exodeoxyribonuclease V; D005090:Exodeoxyribonucleases / Q000502:physiology*; D013329:Structure-Activity Relationship
chemical_list: D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D005090:Exodeoxyribonucleases; D043211:Exodeoxyribonuclease V; C468322:exodeoxyribonuclease V, E coli