title: Isogenic group B streptococci devoid of capsular polysaccharide or beta-hemolysin: pulmonary hemodynamic and gas exchange effects during bacteremia in piglets.
abstract: Group B beta-hemolytic streptococcus (GBS) causes thromboxane (Tx)-associated pulmonary hypertension and hypoxemia in neonatal animals and human infants. The components of GBS that induce these features of sepsis are incompletely characterized. The capsular polysaccharide has been implicated based on the effects of GBS extracts. We used isogenic mutants of a parent GBS strain (COH 31 r/s) devoid of capsular polysaccharide or beta-hemolysin to determine if these components caused the acute features of GBS bacteremia. In neonatal piglets, we observed a similar increase in pulmonary vascular resistance (PVR, mm Hg/L/min) during a 1 h infusion at 5 x 10(8) colony-forming unit/kg/h of COH 31 r/s (n = 5, 11.6 +/- 1.4 to 67.1 +/- 17.9), an isogenic GBS mutant devoid of type III CP (n = 5, 12.5 +/- 1.4 to 56.9 +/- 5.0), and an isogenic GBS mutant devoid of beta-hemolysin (n = 4, 11.0 +/- 1.9 to 51.9 +/- 7.9). All three GBS strains caused increases in blood TxB2 levels, mild arterial hypoxemia, mild reduction in mixed venous PO2, and a 30-40% reduction in cardiac output after a 1 h infusion. The Tx-synthase inhibitor, dazmegrel, completely reversed pulmonary hypertension, and partially reversed arterial hypoxemia and TxB2 levels to baseline values for all GBS strains. In six additional piglets, infusion of polystyrene beads of similar size to GBS at a dose of 5 x 10(8) beads/kg/h caused no changes in gas exchange or blood TxB2 levels, but a mild increase in PVR (13.3 +/- 2.0 to 17.7 +/- 3.5).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Pediatrics, University of Washington School of Medicine, Seattle 98195.
authors.forename: R L
authors.initials: RL
authors.lastname: Gibson
authors.forename: G J
authors.initials: GJ
authors.lastname: Redding
authors.forename: W E
authors.initials: WE
authors.lastname: Truog
authors.forename: W R
authors.initials: WR
authors.lastname: Henderson
authors.forename: C E
authors.initials: CE
authors.lastname: Rubens
mesh_terms: D000818:Animals; D001784:Blood Gas Analysis; D006460:Hemolysin Proteins / Q000502:physiology*; D006976:Hypertension, Pulmonary / Q000209:etiology*; D000860:Hypoxia / Q000209:etiology*; D011135:Polysaccharides, Bacterial; D013290:Streptococcal Infections; D013292:Streptococcus agalactiae / Q000472:pathogenicity*; D013552:Swine; D013929:Thromboxane B2 / Q000097:blood
chemical_list: D006460:Hemolysin Proteins; D011135:Polysaccharides, Bacterial; D013929:Thromboxane B2