Proliferation of immunocytochemically identified islet beta cells in culture: effect of growth factors, hormones, and nutrients.
Immunocytochemically identified, differentiated, single beta-cells proliferate to form colonies, which then grow into hillocks and islets. Beta-Cell proliferation is most easily quantified during the first week, when colonies are forming. The experimental objective was stimulation of beta-cell proliferation by culture medium supplementation with growth factors, hormones, or nutrients. We found that beta-cell proliferation is stimulated by iron-saturated transferrin, interleukin-1-alpha, fetal calf serum, and glucose. In response to transferrin, proliferation of beta-cells is progressively stimulated, reaching a maximum at 30 micrograms/ml. At greater concentrations the stimulatory effect is progressively lost. Interleukin-1-alpha maximally stimulates beta-cell proliferation at 10 pg/ml, regresses to control levels at 10(3) pg/ml, and inhibits proliferation progressively at greater concentrations. Fetal calf serum maximally stimulates beta-cell proliferation at concentrations of 10%, and glucose stimulates maximally at 15 mM concentrations. The proliferative response to transferrin, interleukin-1-alpha or glucose is serum dependent. Serum and transferrin synergistically stimulate glucose-induced beta-cell proliferation. Interleukin-1-beta, interleukin-2, rat growth hormone, and rat prolactin fail to stimulate beta-cell proliferation.
Popiela
H
H
Department of Pediatrics, University of Kansas Medical Center, Kansas.
Moore
W
W
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media; D005947:Glucose / Q000494:pharmacology; D013006:Growth Hormone / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology; D007515:Islets of Langerhans / Q000187:drug effects / Q000254:growth & development* / Q000502:physiology; D011388:Prolactin / Q000494:pharmacology; D051381:Rats; D014168:Transferrin / Q000494:pharmacology
D003470:Culture Media; D007375:Interleukin-1; D014168:Transferrin; D011388:Prolactin; D013006:Growth Hormone; D005947:Glucose