title: Dystrophin in skeletal muscle. I. Western blot analysis using a monoclonal antibody.
abstract: The value of analysing dystrophin on Western blots of skeletal muscle for the differential diagnosis of Xp21 muscular dystrophies is now fairly well established. Here we describe a sensitive system based on monoclonal antibodies to dystrophin. The specificity of the antibodies was established and experiments were undertaken to identify the source of dystrophin-related protein bands which were detected on blots of normal skeletal muscle. These investigations formed a necessary preliminary study to the application of the assay to samples of muscle obtained at biopsy from patients with Duchenne and Becker muscular dystrophy.
authors.affiliation: Muscular Dystrophy Group Research Laboratory, Newcastle General Hospital, Newcastle upon Tyne, U.K.
authors.forename: L V
authors.initials: LV
authors.lastname: Nicholson
authors.forename: K
authors.initials: K
authors.lastname: Davison
authors.forename: G
authors.initials: G
authors.lastname: Falkous
authors.forename: C
authors.initials: C
authors.lastname: Harwood
authors.forename: E
authors.initials: E
authors.lastname: O'Donnell
authors.forename: C R
authors.initials: CR
authors.lastname: Slater
authors.forename: J B
authors.initials: JB
authors.lastname: Harris
mesh_terms: D000911:Antibodies, Monoclonal; D001706:Biopsy; D015153:Blotting, Western; D016189:Dystrophin; D006801:Humans; D008970:Molecular Weight; D009124:Muscle Proteins / Q000276:immunology* / Q000302:isolation & purification; D009132:Muscles / Q000378:metabolism*; D009136:Muscular Dystrophies / Q000175:diagnosis* / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D016189:Dystrophin; D009124:Muscle Proteins