Detection of cryptic bands by AluI in eukaryotic chromosomes.
Selective digestion of fixed chromatin with the restriction endonuclease AluI (which cuts the sequence AG CT) uncovers a specific and repeatable pattern of bands within the euchromatin of two species of grasshoppers and of the L929 mouse cell line, which are not detectable by means of other banding techniques such as C-bands, specific fluorochromes, or other restriction endonucleases. It is tentatively suggested that this chromatin represents a special class of repetitive DNA embedded in the euchromatin, not containing the AluI restriction site to the same extent as in euchromatin and not associated with C-banded heterochromatic material.
Gosálvez
J
J
Departamento de Biología, Facultad de Ciencias C-XV, Universidad Autónoma de Madrid, Spain.
López-Fernández
C
C
Goyanes
V
V
D000818:Animals; D002843:Chromatin / Q000032:analysis*; D002871:Chromosome Banding; D015252:Deoxyribonucleases, Type II Site-Specific; D006110:Grasshoppers / Q000235:genetics; D051379:Mice; D012091:Repetitive Sequences, Nucleic Acid
D002843:Chromatin; C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific