title: A marker and putative pathoantigen of Hodgkin's cells.
abstract: A galactose-specific lectin, recently described by our laboratory, is immunologically demonstrable on the surface of neoplastic cells derived from patients with Hodgkin's disease. This Hodgkin's lectin is shown to be functionally and antigenically related to the galactose-N-acetylgalactosamine-specific lectin of the hepatocyte (HBP). Poly- and monoclonal antibodies against either the cytoplasmic tail or the cell-surface binding site of HBP recognize the Hodgkin's lectin as a 55 Kd protein. Expression of the 55 Kd antigen appears to be restricted to Hodgkin's disease involved tissues and cells of the monocyte/macrophage lineage. The putative identification of the Hodgkin's lectin as an ectosialyltransferase unique to Hodgkin's cells is supported by inhibition of enzymatic activity by anti-HBP antibodies. Cultured Hodgkin's cells, in analogy to purified HBP, agglutinate T-lymphocytes mediated by the Hodgkin's lectin. This cell-to-cell interaction results in the incorporation of sialic acid into lymphocyte surface asialoglycans as well as in the stimulation of lymphocyte proliferation. The function of the Hodgkin's lectin as lymphocyte agglutinant in vitro suggests its role as an immunomodulator contributing to the immunodeficiencies associated with Hodgkin's disease.
authors.affiliation: Department of Oncology, Montefiore Medical Center, New York, NY 10467.
authors.forename: E
authors.initials: E
authors.lastname: Paietta
authors.forename: R J
authors.initials: RJ
authors.lastname: Stockert
authors.forename: A
authors.initials: A
authors.lastname: Morell
authors.forename: J
authors.initials: J
authors.lastname: Racevskis
authors.forename: P H
authors.initials: PH
authors.lastname: Wiernik
mesh_terms: D000951:Antigens, Neoplasm / Q000276:immunology*; D014408:Biomarkers, Tumor / Q000276:immunology*; D006689:Hodgkin Disease / Q000276:immunology* / Q000473:pathology; D006801:Humans
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor