Biological and molecular evidence for the production of IL-6 by human natural killer cells in culture.
As several of the biological functions of NK cells are similar to the biological effects of IL-6, we tested for the production of this cytokine by cultured NK cells. Conditioned medium from the NK cells supported the proliferation of an IL-6 dependent human leukemic plasma cell line in dose-dependent fashion and this response was abolished by a neutralizing anti-IL-6 serum. Analysis of the mRNA from the NK cell cultures by RNA blotting demonstrated a specific transcript for IL-6 providing further confirmation that these cells elaborate this factor in culture.
Avanzi
G C
GC
Istituto di Medicina Interna, Universitå di Torino, Italy.
Cessano
A
A
Brizzi
M F
MF
Clark
S C
SC
Pegoraro
L
L
Matera
L
L
D015152:Blotting, Northern; D002455:Cell Division; D002478:Cells, Cultured; D006801:Humans; D007158:Immunologic Techniques; D015850:Interleukin-6 / Q000096:biosynthesis*; D007694:Killer Cells, Natural / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
D015850:Interleukin-6; D012333:RNA, Messenger