The application of nuclear magnetic resonance spectroscopy to drug metabolism studies.
1. The applications of n.m.r. spectroscopy studies to drug metabolism, both in vitro (biofluids, isolated cells, excised tissue samples, isolated organs) and in vivo (animals, humans) are reviewed. 2. N.m.r. is a relatively insensitive technique, but it has the great advantage of being non-invasive and non-destructive, i.e. it allows a direct study of intact biological samples. 3. The majority of studies examined deal with 19F-n.m.r. spectroscopy mainly because of the favourable n.m.r. characteristics of this nucleus, and the low level of endogenous fluorine which gives no detectable 19F signal. However, the potential utility of 1H-31P- and 13C-n.m.r. is also emphasized.
Malet-Martino
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Groupe de RMN Biomédicale, Laboratoire des IMRCP, Université Paul Sabatier, Toulouse, France.
Martino
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D000818:Animals; D005472:Fluorouracil / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D013329:Structure-Activity Relationship
D004364:Pharmaceutical Preparations; D005472:Fluorouracil