[The influences of endogenous bile acids on maltase and alkaline phosphatase activities in intrahepatic cholestasis rats].
The activities of lysosomal maltase in the serum, bile and liver were determined in intrahepatic cholestasis rats induced by alpha-naphtylisothiocyanate (ANIT, 200 mg/kg, i.p.), and compared with changes in alkaline phosphatase (ALP) activity. Moreover, the influences of endogenous bile acids on the release of maltase activity from the liver in intrahepatic cholestasis rats were studied. The maltase activities in the serum and bile significantly increased from 4 and 8 h after the intraperitoneal administration of ANIT, respectively. Conversely, a significant decrease in liver maltase activity was observed from 4 h after the injection of ANIT. On the other hand, total bile acid concentrations in the serum and bile significantly increased immediately after the treatment of ANIT, when biliary bile acid, exogenous bile acid or Triton X-100 was added to lysosomal fraction in the liver, the maltase activity in the supernate after the reaction significantly increased in proportion to the concentration of each substance added to the liver lysosome. These results suggested that maltase might be released from liver lysosomal membrane by surface active-action of bile acid accumulated in the liver after the administration of ANIT. Moreover, the changes in ALP activities in the serum, bile and liver after the administration of ANIT were almost similar to those in maltase activity.
Iizuka
Y
Y
Sakurai
E
E
Hikichi
N
N
Niwa
H
H
D015058:1-Naphthylisothiocyanate / Q000494:pharmacology; D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism / Q000502:physiology*; D002780:Cholestasis, Intrahepatic / Q000201:enzymology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D000520:alpha-Glucosidases / Q000378:metabolism*
D001647:Bile Acids and Salts; D015058:1-Naphthylisothiocyanate; D000469:Alkaline Phosphatase; D000520:alpha-Glucosidases