title: Synthesis and secretion of bacterial alpha-amylase by the yeast Saccharomyces cerevisiae.
abstract: Alpha-amylase from Bacillus amyloliquefaciens, synthesized in yeast Saccharomyces cerevisiae without substitution of the signal sequence, is efficiently secreted from yeast cells: 60-70% of the overall amount of the enzyme is found in the culture fluid. In contrast to many yeast secretory proteins, which accumulate in the periplasmic space and in the cell wall, intracellular alpha-amylase is localized mainly in the cytoplasm. Obviously, transfer across the cell wall is not a rate-limiting step in alpha-amylase export from the cell. The glycosylated forms of proteins are predominantly found both inside the cell and in the culture medium.
authors.affiliation: Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, USSR.
authors.forename: I E
authors.initials: IE
authors.lastname: Kovaleva
authors.forename: L A
authors.initials: LA
authors.lastname: Novikova
authors.forename: V N
authors.initials: VN
authors.lastname: Luzikov
mesh_terms: D001407:Bacillus / Q000201:enzymology* / Q000235:genetics; D002473:Cell Wall / Q000201:enzymology; D003470:Culture Media; D003593:Cytoplasm / Q000201:enzymology; D004274:DNA, Recombinant; D006031:Glycosylation; D021382:Protein Sorting Signals / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D000516:alpha-Amylases / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism
chemical_list: D003470:Culture Media; D004274:DNA, Recombinant; D021382:Protein Sorting Signals; D000516:alpha-Amylases