A spectrin-like protein present on membranes of Amoeba proteus as studied with monoclonal antibodies.
Monoclonal antibodies against a spectrin-like membrane-associated protein of xD amoebae. (Amoeba proteus) were used to determine the distribution of the protein and some of its characteristics. A total of 34 monoclonal antibodies recognizing different epitopes of the protein were obtained, of which seven stained cell membranes by indirect immunofluorescence. The spectrin-like protein had two subtypes of 225 and 220 kDa and several monoclonal antibodies cross-reacted with human erythrocyte spectrin when checked by indirect immunofluorescence staining and immunoblotting. Some of the antibodies also cross-reacted with antigens in HeLa cells and chick embryo fibroblasts. Polyclonal and monoclonal antibodies against Drosophila and human erythrocyte spectrins cross-reacted with the spectrin-like protein from amoebae. On the basis of these results, it was concluded that the protein is a spectrin. The protein was found on most cellular membranes of amoebae, including the plasma, nuclear, and phagosomal membranes, as well as symbiosome membranes.
Choi
E Y
EY
Department of Zoology, University of Tennessee, Knoxville 37996.
Jeon
K W
KW
D000656:Amoeba / Q000166:cytology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002462:Cell Membrane / Q000378:metabolism; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D007425:Intracellular Membranes / Q000378:metabolism; D009685:Nuclear Envelope / Q000378:metabolism; D013049:Spectrin / Q000032:analysis / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D013049:Spectrin