Antibodies to bovine serum albumin in swine sera: implications for false-positive reactions in the serodiagnosis of African swine fever.
Antibodies to bovine serum albumin were detected in swine sera by use of an immunoblotting technique. Such sera had false-positive reactions, as determined by results of African swine fever virus serodiagnostic techniques when bovine serum albumin was a contaminant in the soluble cytoplasmic antigen obtained from infected cells cultured in the presence of bovine serum. The soluble cytoplasmic antigen obtained from cell cultures infected with African swine fever virus in the presence of porcine serum did not react with the false-positive sera and, therefore, was used for African swine fever virus serodiagnostic methods, with 0% false-positive results.
Escribano
J M
JM
Departmento de Sanidad Animal, Instituto Nacional de Investigaciones Agrarias, Madrid, Spain.
Pastor
M J
MJ
Sánchez-Vizcaíno
J M
JM
D000357:African Swine Fever / Q000175:diagnosis*; D000818:Animals; D000906:Antibodies / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005189:False Positive Reactions; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D015151:Immunoblotting; D012698:Serologic Tests / Q000662:veterinary; D012710:Serum Albumin, Bovine / Q000276:immunology*; D013552:Swine
D000906:Antibodies; D012710:Serum Albumin, Bovine