title: Estradiol-concentrating cells in the brains of hypogonadal female mice and in their intraventricular preoptic area implants.
abstract: Estradiol-concentrating cells were evaluated in the brains of hypogonadal female mice and in their intraventricular preoptic area brain grafts using autoradiography for [3H]estradiol. Normal distribution of estradiol-concentrating cells was observed in the brains of the hypogonadal mice with dense collections of these cells in the lateral septum; the medial preoptic area; the medial anterior hypothalamus; the ventromedial, arcuate, and periventricular nuclei of the hypothalamus; and the medial and cortical nuclei of the amygdala. In addition, estradiol-concentrating cells were present in all the transplants, with the estimated number of such cells in the transplants ranging from 390 to 2600. There was no correlation between numbers of estradiol-concentrating cells within the transplants and degree of reproductive recovery in the hypogonadal mice. Gonadotropin-releasing hormone (GnRH) immunocytochemistry of alternate sections revealed GnRH-immunoreactive material within the grafts and immunoreactive fibers exiting the grafts and entering the hosts' median eminence. No specific relationship between GnRH cells and estradiol-concentrating cells was evident within the grafts, nor was there any indication of identity of estrogen-concentrating cells with GnRH cells.
authors.affiliation: Department of Medicine, Mount Sinai School of Medicine, New York, New York 10029.
authors.forename: M J
authors.initials: MJ
authors.lastname: Gibson
authors.forename: A J
authors.initials: AJ
authors.lastname: Silverman
authors.forename: M F
authors.initials: MF
authors.lastname: Rosenthal
authors.forename: J I
authors.initials: JI
authors.lastname: Morrell
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001345:Autoradiography; D001921:Brain / Q000378:metabolism*; D002552:Cerebral Ventricles / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000502:physiology; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D007006:Hypogonadism / Q000378:metabolism* / Q000503:physiopathology; D007150:Immunohistochemistry; D051379:Mice; D008815:Mice, Inbred Strains; D010906:Pituitary Hormone-Releasing Hormones / Q000378:metabolism; D011301:Preoptic Area / Q000196:embryology / Q000378:metabolism / Q000637:transplantation*; D012098:Reproduction
chemical_list: D010906:Pituitary Hormone-Releasing Hormones; D004958:Estradiol