Expression of human insulin and hybrid insulin genes in pancreatic beta cells of transgenic mice.
Transgenic mice which specifically express the human insulin gene in the pancreatic beta cells were obtained by microinjection of DNA into fertilized one-cell embryos. The elements regulating in vivo the beta-cell specificity of the insulin gene, located within a few hundred base pairs upstream to the start site of transcription, were used to construct hybrid genes expressed in pancreatic beta cells. Expression of SV40-T large antigen resulted in beta-cell proliferation and beta-cell tumors, while major histocompatibility, or interferon-gamma molecules induced diabetes mellitus in transgenic mice.
Bucchini
D
D
Institut Jacques-Monod, INSERM, U. 257, CNRS, Paris, France.
Deltour
L
L
Jami
J
J
D000818:Animals; D015870:Gene Expression; D006801:Humans; D007328:Insulin / Q000096:biosynthesis / Q000235:genetics*; D007515:Islets of Langerhans / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic
D007328:Insulin