title: Preliminary crystallographic study of glycosylated recombinant human renin.
abstract: Single crystals of glycosylated recombinant human renin have been obtained using the hanging-drop vapor diffusion method with polyethylene glycol and sodium chloride as coprecipitants. The crystals belong to the cubic space group P2(1)3 with a = 143.0 A and contain two molecules of renin in the asymmetric unit. A self-rotation function study using 5.5 A data shows the orientation of a non-crystallographic 2-fold axis relating these two monomers.
authors.affiliation: Corporate Research and Development, Monsanto, Chesterfield Village, MO 63198.
authors.forename: L W
authors.initials: LW
authors.lastname: Lim
authors.forename: R A
authors.initials: RA
authors.lastname: Stegeman
authors.forename: N K
authors.initials: NK
authors.lastname: Leimgruber
authors.forename: J K
authors.initials: JK
authors.lastname: Gierse
authors.forename: S S
authors.initials: SS
authors.lastname: Abdel-Meguid
mesh_terms: D006031:Glycosylation; D006801:Humans; D011994:Recombinant Proteins / Q000378:metabolism / Q000648:ultrastructure; D012083:Renin / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014961:X-Ray Diffraction
chemical_list: D011994:Recombinant Proteins; D012083:Renin