title: Expression in Escherichia coli of catalytically active phenylalanine ammonia-lyase from parsley.
abstract: In parsley (Petroselinum crispum), phenylalanine ammonia-lyase (PAL) is encoded by 4 structurally similar genes. The nucleotide sequence of a near full-length cDNA and the deduced amino acid sequence of PAL-4 are presented and compared with the corresponding sequences of PAL-1, a previously described representative of the gene family. Transformation of Escherichia coli cells with PAL-1 or PAL-4 cDNA yielded catalytically active PAL, suggesting that the catalytic center of the enzyme is formed spontaneously rather than by a plant-specific mechanism.
authors.affiliation: Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Kln, FRG.
authors.forename: W
authors.initials: W
authors.lastname: Schulz
authors.forename: H G
authors.initials: HG
authors.lastname: Eiben
authors.forename: K
authors.initials: K
authors.lastname: Hahlbrock
mesh_terms: D000642:Ammonia-Lyases / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010650:Phenylalanine Ammonia-Lyase / Q000235:genetics* / Q000378:metabolism; D010944:Plants / Q000201:enzymology / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D015345:Oligonucleotide Probes; D000642:Ammonia-Lyases; D010650:Phenylalanine Ammonia-Lyase