Structure of the elastin gene and alternative splicing of elastin mRNA: implications for human disease.
The protein elastin is largely responsible for the elastic properties of vertebrate lungs, large blood vessels, and skin. The structure of the human, bovine, and chick elastin gene and protein monomer, tropoelastin, has recently been elucidated by using techniques of molecular biology. Extensive homology of amino acid sequence exists among the mammalian species and there is in addition strong conservation of nucleotide sequences in the 3' untranslated region of the gene. The translated exons are small and embedded in large expanses of introns. Sequences coding for the hydrophobic regions, responsible for the elastic properties of the molecule, and the alanine-lysine rich regions, responsible for crosslink formation between molecules, reside in separate exons and alternate for the most part in the elastin gene. S1 analyses and sequence analysis of cDNA and genomic clones have indicated that there is substantial alternative splicing of the primary elastin transcript. Variations in the structure of mRNAs resulting from alternative splicing could explain the existence of the multiple forms of tropoelastin observed electrophoretically in several species. Different kinds of splicing patterns could occur in human populations and may contribute to aging and pathological situations in the cardiovascular and pulmonary systems.
Indik
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Department of Anatomy and Histology, School of Dental Medicine, University of Pennsylvania, Philadelphia 19104.
Yeh
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H
Ornstein-Goldstein
N
N
Kucich
U
U
Abrams
W
W
Rosenbloom
J C
JC
Rosenbloom
J
J
D000375:Aging / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004549:Elastin / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics*; D014333:Tropoelastin / Q000235:genetics
D012333:RNA, Messenger; D014333:Tropoelastin; D004549:Elastin