Heat-shock protein 83 of Leishmania mexicana amazonensis is an abundant cytoplasmic protein with a tandemly repeated genomic arrangement.
The 83-kDa heat-shock protein HSP83 is a highly abundant protein in Leishmania amastigotes and promastigotes exposed to elevated temperature. Antibodies against this protein were obtained by immunization with a synthetic peptide derived from a conserved region. These antibodies recognized both the denatured and the native form of the molecule and were used for immunofluorescence analysis. These experiments, together with analysis by cell fractionation, show that HSP83 is distributed in the cytoplasm of Leishmania parasites. The gene for HSP83 in Leishmania mexicana amazonensis has been cloned from a genomic library, and molecular characterization shows it is present in several copies of 4-kb repeats arranged in tandem.
Shapira
M
M
Department of Chemical Immunology, MacArthur Center for Molecular Parasitology, Weizmann Institute of Science, Rehovot, Israel.
Pinelli
E
E
D000818:Animals; D000918:Antibody Specificity; D002458:Cell Fractionation; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000378:metabolism; D004247:DNA / Q000235:genetics; D015723:Gene Library; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D015151:Immunoblotting; D007158:Immunologic Techniques; D007894:Leishmania mexicana / Q000235:genetics* / Q000378:metabolism; D010455:Peptides / Q000235:genetics / Q000276:immunology; D011817:Rabbits; D012091:Repetitive Sequences, Nucleic Acid
D006360:Heat-Shock Proteins; D010455:Peptides; D004247:DNA