[Level of Hu protein binding with DNA and the Hu/DNA ratio in Escherichia coli cells with various generation periods].
The possibility of quantitative determination of protein HU in E. coli cell lysates was demonstrated, using enzyme immunoassay with monospecific polyclonal antibodies against HU and homogeneous protein HU. The protein HU/DNA ratio in two cultures of E. coli with different generation periods was found to be constant despite the differences in the levels of proteins HU and DNA. Protein HU was found to be represented by approximately 420.10(3) copies per fast growing. E. coli cell and by 320.10(3) copies per slowly growing cell. These amounts of the HU protein correspond to a ratio of one protein HU molecule per 44 +/- 8 base pairs of DNA and can provide for the nucleosome-like organization of approximately 30% of E. coli DNA length. The constant HU/DNA ratio which is similar to constant histones/DNA ratio provides additional evidence in favour of functional similarity of the HU E. coli DNA-binding protein to histones.
Kulyba
N P
NP
Kozlov
A V
AV
D001426:Bacterial Proteins / Q000378:metabolism*; D002846:Chromatography, Affinity; D004269:DNA, Bacterial / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D005455:Fluorescent Antibody Technique
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; C040330:histone-like protein HU, bacteria
Soderzhanie sviazyvaiushchegosiia s DNK belka Hu i sootnoshenie Hu/DNK v kletkakh Escherichia coli s raznym periodom generatsii.