title: Gene function identified by interspecific transformation.
abstract: Aspergillus nidulans is able to utilize 2-pyrrolidinone as a nitrogen source while two related Aspergillus species, A. niger and A. terreus, cannot. Mutations in the lamA gene of A. nidulans prevent growth on 2-pyrrolidinone. A plasmid (pLAM7) has been isolated containing the A. nidulans lamA gene and a divergently transcribed adjacent gene of unknown function. Transformation of A. terreus with subclones of pLAM7 showed that both genes are essential for the utilization of a new nitrogen source, 2-pyrrolidinone, in that species. The previously unidentified gene has been designated lamB.
authors.affiliation: Department of Genetics, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: M E
authors.initials: ME
authors.lastname: Katz
authors.forename: M J
authors.initials: MJ
authors.lastname: Hynes
mesh_terms: D001230:Aspergillus / Q000235:genetics* / Q000378:metabolism; D001233:Aspergillus nidulans / Q000235:genetics / Q000378:metabolism; D001234:Aspergillus niger / Q000235:genetics / Q000378:metabolism; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D005800:Genes, Fungal; D009154:Mutation; D010957:Plasmids; D011760:Pyrrolidinones / Q000378:metabolism; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014170:Transformation, Genetic
chemical_list: D011760:Pyrrolidinones