title: Crystallization and characterization of two crystal forms of the B800-850 light-harvesting complex from Rhodopseudomonas acidophila strain 10050.
abstract: Two different crystal forms of the B800-850-antenna complex from Rhodopseudomonas acidophila strain 10050 have been grown. This complex is an integral membrane protein and is isolated as an oligomeric assembly with a molecular weight of approximately 84 kDa. This assembly contains six alpha/beta apoprotein pairs, 18 molecules of bacteriochlorophyll a and nine molecules of carotenoid. The first crystal form has dimensions unit cell a = b = 75.8 A, c = 97.5 A with the space group P4 and diffracts to a resolution of 12.0 A. The second crystal form is rhombohedral with dimensions unit cell a = 121.1 A, alpha = 60 degrees, space group R32 and diffracts to a resolution of 3.5 A. Native data have been processes in both cases, to an Rmerge value of 9.0 to 11.0%. The X-ray data suggest that the asymmetric unit, in both crystal forms, contains one 84 kDa antenna complex.
authors.affiliation: Department of Botany, University of Glasgow, Scotland.
authors.forename: M Z
authors.initials: MZ
authors.lastname: Papiz
authors.forename: A M
authors.initials: AM
authors.lastname: Hawthornthwaite
authors.forename: R J
authors.initials: RJ
authors.lastname: Cogdell
authors.forename: K J
authors.initials: KJ
authors.lastname: Woolley
authors.forename: P A
authors.initials: PA
authors.lastname: Wightman
authors.forename: L A
authors.initials: LA
authors.lastname: Ferguson
authors.forename: J G
authors.initials: JG
authors.lastname: Lindsay
mesh_terms: D001059:Apoproteins / Q000032:analysis; D001426:Bacterial Proteins / Q000032:analysis*; D001429:Bacteriochlorophylls / Q000032:analysis; D002338:Carotenoids / Q000032:analysis; D003460:Crystallization; D045342:Light-Harvesting Protein Complexes; D008565:Membrane Proteins / Q000032:analysis*; D045322:Photosynthetic Reaction Center Complex Proteins
chemical_list: D001059:Apoproteins; D001426:Bacterial Proteins; D001429:Bacteriochlorophylls; D045342:Light-Harvesting Protein Complexes; D008565:Membrane Proteins; D045322:Photosynthetic Reaction Center Complex Proteins; C002142:carotenoic acid; D002338:Carotenoids