Detection of conglutinin in bovine serum by complement-dependent agglutination of Escherichia coli.
Agglutination of Escherichia coli (ECA) by normal bovine serum was shown to be prevented by heating serum to 56 degrees C for 30 min, but restored by normal horse, swine, rabbit or guinea pig sera. Further investigation of the ECA reaction using techniques to distinguish between conglutination and immunoconglutination indicated ECA to be a conglutination reaction. Testing of 264 sera obtained from 22 normal cattle over a period of 5 months did not show individual or seasonal variation in ECA. Changes in ECA and conglutination were detected in sera of periparturient cows. The ECA reaction is a simple technique for detecting conglutinin in bovine serum.
Thurston
J R
JR
National Animal Disease Center, Ames, IA 50010.
Kehrli
M E
ME
Driftmier
K
K
Deyoe
B L
BL
D000371:Agglutination; D000818:Animals; D002417:Cattle / Q000097:blood* / Q000276:immunology; D037222:Collectins; D003168:Complement Fixation Tests; D003165:Complement System Proteins; D004926:Escherichia coli / Q000276:immunology; D005260:Female; D011247:Pregnancy; D012712:Serum Globulins / Q000032:analysis*
D037222:Collectins; D012712:Serum Globulins; C024014:conglutinin; D003165:Complement System Proteins