title: [Synthesis of oligoribonucleotides with the use of RNA polymerases of E. coli and immobilized synthetic DNA-templates].
abstract: A new approach to synthesis of oligoribonucleotides is suggested, based on transcription by E. coli RNA polymerase of synthetic immobilized DNA-templates with AUG as primer. The approach has been experimentally verified by synthesis of two oligonucleotides, viz., a RNA fragment of the fr phage (16 nucleotides long) and a RNA fragment of the tickborne encephalitis virus (18 nucleotides long). Fraction of the synthesized RNA fragments in the whole nucleotide material is about 20%. The templates can be used repeatedly. Sequences of the oligoribonucleotides were confirmed. Advantages of this approach and its usefulness for SP6 DNA-dependent RNA polymerase are discussed.
authors.forename: L Ia
authors.initials: LIa
authors.lastname: Denisova
authors.forename: S N
authors.initials: SN
authors.lastname: Zagrebel'nyy
authors.forename: N M
authors.initials: NM
authors.lastname: Pustoshilova
authors.forename: A G
authors.initials: AG
authors.lastname: Ven'iaminova
authors.forename: V V
authors.initials: VV
authors.lastname: Gorn
mesh_terms: D001345:Autoradiography; D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology*; D008969:Molecular Sequence Data; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D013698:Templates, Genetic
chemical_list: D009843:Oligoribonucleotides; D012321:DNA-Directed RNA Polymerases
vernacular_title: Sintez oligoribonukleotidov s ispol'zovaniem RNK-polimerazy E. coli i immobilizovannykh sinteticheskikh DNK-matrits.