Capping and co-capping of membrane immunoglobulin and lipid-conjugated immunoglobulin inserted in the cell membrane of B lymphocytes.
Human and mouse immunoglobulins (Ig) or F(ab')2 fragments of rabbit Ig were conjugated to lipids and the conjugates inserted into the membrane of mouse spleen cells. It was found that nearly all B cells, but not T cells, became decorated with lipid immunoglobulin. Both endogenous Ig receptors and inserted Ig capped after the addition of cross-linking F(ab')2 antibodies and in both cases capping required energy. Capping of endogenous mouse Ig led to co-capping of inserted human Ig, but the reverse was not true.
Faro-Rivas
J
J
Department of Immunology, University of Stockholm, Sweden.
Clinchy
B
B
Höidén
I
I
Möller
G
G
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002462:Cell Membrane / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007152:Immunologic Capping; D051379:Mice; D008815:Mice, Inbred Strains; D010168:Palmitates; D010169:Palmitic Acids; D011947:Receptors, Antigen, B-Cell / Q000276:immunology*; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology
D007140:Immunoglobulin Fab Fragments; D010168:Palmitates; D010169:Palmitic Acids; D011947:Receptors, Antigen, B-Cell