Induction of cells with a chondrocyte-like phenotype by treatment with 1 alpha,25-dihydroxyvitamin D3 in a human salivary acinar cell line.
A clonal cell with an acinar cell phenotype, which was induced by 5-azacytidine treatment of a neoplastic human salivary intercalated duct cell line, was cultivated in the presence of 1 alpha,25-dihydroxyvitamin D3. Morphological changes occurred; large cells that were polygonal or round in shape and had numerous vacuoles in their cytoplasm appeared in the treated cells, whereas the same concentration of 1 alpha,25-dihydroxyvitamin D3 did not affect the morphology of the parental cells. Major alterations, such as expression of type II collagen, alpha and beta chains of S-100 protein, and sulfated proteoglycans, were observed in these cells with a phenotype similar to chondrocytes. After the removal of 1 alpha,25-dihydroxyvitamin D3 from the culture, the treated cells returned rapidly to the phenotype of the untreated cells. These findings indicate that the reversible differentiation into chondrocyte-like cells of a human salivary acinar cell line occurs in growth medium containing 1 alpha,25-dihydroxyvitamin D3.
Azuma
M
M
Second Department of Oral and Maxillofacial Surgery, Tokushima University School of Dentistry, Japan.
Kawamata
H
H
Kasai
Y
Y
Yanagawa
T
T
Yoshida
H
H
Sato
M
M
D002117:Calcitriol / Q000494:pharmacology*; D002356:Cartilage / Q000032:analysis / Q000473:pathology* / Q000648:ultrastructure; D002454:Cell Differentiation / Q000187:drug effects; D003094:Collagen / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006801:Humans; D010641:Phenotype; D011509:Proteoglycans / Q000032:analysis; D009418:S100 Proteins / Q000032:analysis; D012468:Salivary Gland Neoplasms / Q000032:analysis / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000032:analysis / Q000473:pathology / Q000648:ultrastructure
D011509:Proteoglycans; D009418:S100 Proteins; D003094:Collagen; D002117:Calcitriol