Inhibition of human macrophage colony formation by interleukin 4.
We investigated the effects of human rIL-4 on in vitro hematopoiesis. A profound inhibition of macrophage colony formation by IL-4 was observed, whereas colony growth of other lineages was not affected. Inhibition of macrophage colony growth was not restricted to GM-CSF-induced colony growth but was also present in cultures stimulated with M-CSF. This inhibition was not only observed in cultures of light density bone marrow cells, but also in cultures of monocyte- and T lymphocyte-depleted bone marrow cells. Since a similar inhibition was observed in cultures of CD34+HLA-DR+-enriched bone marrow cells, a direct action of IL-4 on monocyte-committed progenitor cells is suggested.
Jansen
J H
JH
Department of Hematology, Leiden University Medical Center, The Netherlands.
Wientjens
G J
GJ
Fibbe
W E
WE
Willemze
R
R
Kluin-Nelemans
H C
HC
D000938:Antigen-Presenting Cells / Q000276:immunology; D001854:Bone Marrow Cells; D003115:Colony-Stimulating Factors / Q000037:antagonists & inhibitors; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006131:Growth Inhibitors; D006410:Hematopoiesis / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D015847:Interleukin-4; D007378:Interleukins / Q000494:pharmacology*; D016173:Macrophage Colony-Stimulating Factor; D008264:Macrophages / Q000502:physiology*; D011994:Recombinant Proteins
D003115:Colony-Stimulating Factors; D006131:Growth Inhibitors; D007378:Interleukins; D011994:Recombinant Proteins; D015847:Interleukin-4; D016173:Macrophage Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor