title: Phenotyping of pig alpha 1B-glycoprotein (PO2) and haemopexin by 1D polyacrylamide gel electrophoresis and immunoblotting.
abstract: Described is an alternative procedure for the phenotyping of pig alpha 1B-glycoprotein (PO2) and haemopexin. The procedure is based on the separation of serum samples by horizontal polyacrylamide gel electrophoresis, passive blotting onto a nitrocellulose (NC) sheet, and immunochemical detection using a mixture of a primary antibody (rabbit anti-pig alpha 1B or anti-pig haemopexin) and a peroxidase-labelled secondary antibody. Several NC copies can be obtained from a single gel and these can be developed with different monospecific antisera.
authors.affiliation: Czechoslovak Academy of Sciences, Department of Genetics, Libĕchov.
authors.forename: P
authors.initials: P
authors.lastname: Kaláb
authors.forename: A
authors.initials: A
authors.lastname: Stratil
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D006466:Hemopexin / Q000235:genetics*; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D010641:Phenotype; D013552:Swine
chemical_list: D001798:Blood Proteins; D006466:Hemopexin