Desmin-containing intermediate filaments: structural analysis using monoclonal antibodies.
1. Monoclonal antibodies have been raised against N- and C-terminal desmin sequence regions. 2. The antibodies decorated desmin intermediate filaments in a helical fashion with four antibody molecules per helix turn. 3. The filaments could be decorated with both types of antibody consecutively. 4. These results support a model of intermediate filament assembly in which the tetrameric protofilaments are aligned in a staggered fashion with partial overlapping of the central rod domain regions of the desmin sequence.
Allen
M J
MJ
Department of Chemistry, Ohio University, Athens 45701.
Johnson
P
P
D000818:Animals; D000911:Antibodies, Monoclonal; D002645:Chickens; D003599:Cytoskeleton / Q000648:ultrastructure*; D003893:Desmin / Q000648:ultrastructure*; D007158:Immunologic Techniques; D007382:Intermediate Filaments / Q000648:ultrastructure*; D008854:Microscopy, Electron; D015394:Molecular Structure
D000911:Antibodies, Monoclonal; D003893:Desmin