An improved embedding method for obtaining semithin and ultrathin sections from critical-point dried trachea specimens.
Critical-point dried trachea specimens were rinsed for 48 h in dimethylsulphoxide before being embedded in Epon 812 to obtain semithin and ultrathin sections. Compared with methods hitherto published, this treatment leads to better resin infiltration of the specimens and better preservation of intracellular structures.
Brockmeyer
C
C
Institut für Experimentelle Pathologie, Medizinische Hochschule Hannover, F.R.G.
Thiedemann
K U
KU
Mohr
U
U
D000818:Animals; D006224:Cricetinae; D004121:Dimethyl Sulfoxide; D006652:Histological Techniques; D008647:Mesocricetus; D008855:Microscopy, Electron, Scanning / Q000379:methods; D008867:Microtomy; D013048:Specimen Handling; D014132:Trachea / Q000648:ultrastructure*
D004121:Dimethyl Sulfoxide