Isolation and characterization of ganglioside 9-O-acetyl-GD3 from bovine buttermilk.
Bovine buttermilk contains a unique ganglioside, 9-O-acetyl-GD3. In order to isolate large quantities of this ganglioside, a simplified isolation scheme which consists of several ion-exchange and silica gel column chromatographic procedures was devised. The isolated 9-O-acetyl-GD3 was characterized on the basis of its thin-layer chromatographic behavior, its immunoreactivity with a specific monoclonal antibody, JONES, and by conversion to authentic GD3 by mild base treatment.
Bonafede
D M
DM
Department of Biology, Yale University, New Haven, CT 06511.
Macala
L J
LJ
Constantine-Paton
M
M
Yu
R K
RK
D064753:Ammonium Hydroxide; D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D005732:Gangliosides / Q000302:isolation & purification*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006878:Hydroxides; D007124:Immunoenzyme Techniques; D008545:Melanoma / Q000032:analysis; D008892:Milk / Q000032:analysis*
D005732:Gangliosides; D006878:Hydroxides; D064753:Ammonium Hydroxide; C049132:9-O-acetyl-GD3 ganglioside