title: Molecular characterization of two cya mutations, cya-854 and cyaR1.
abstract: The cya-854 mutation of Escherichia coli K-12 (E. Brickman, L. Soll, and J. Beckwith, J. Bacteriol. 116:582-587, 1973) has been shown to consist of a 200-base-pair deletion generating a new translation start that permits synthesis of the adenylate cyclase carboxy terminus. Contrary to expectations (C. Guidi-Rontani, A. Danchin, and A. Ullmann, J. Bacteriol. 148:753-761, 1981), cyaR1 results from the insertion of an IS1 element into the sequence coding for the catalytic center of the protein.
authors.affiliation: Unité de Régulation de l'Expression Génétique, Institut Pasteur, Paris, France.
authors.forename: P
authors.initials: P
authors.lastname: Glaser
authors.forename: A
authors.initials: A
authors.lastname: Roy
authors.forename: A
authors.initials: A
authors.lastname: Danchin
mesh_terms: D000262:Adenylyl Cyclases / Q000235:genetics*; D001483:Base Sequence; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009154:Mutation; D015183:Restriction Mapping
chemical_list: D000262:Adenylyl Cyclases