Granulocyte chemotactic protein/interleukin-8 induces plasma leakage and neutrophil accumulation in rabbit skin.
Granulocyte chemotactic protein/Interleukin-8 (GCP/IL-8), purified to homogeneity from endotoxin- or mitogen-stimulated human mononuclear cells, was injected intradermally into rabbits to evaluate the proinflammatory properties of this novel cytokine. In the presence of a vasodilator substance, pmol amounts of GCP/IL-8 induced neutrophil accumulation that was fast in onset, relatively short of duration (half life 60 to 70 minutes), and was associated with a parallel time course of plasma protein extravasation. GCP/IL-8-induced edema formation was found to be neutrophil dependent. These data provide evidence that GCP/IL-8 fulfills two important criteria for consideration as an inflammatory mediator. It is possible that endogenous GCP/IL-8, if produced locally by tissue macrophages, may contribute to the initiation of the inflammatory response to infection.
Rampart
M
M
Laboratory of Experimental Pharmacology, University of Antwerp, Belgium.
Van Damme
J
J
Zonnekeyn
L
L
Herman
A G
AG
D000818:Animals; D002630:Chemotactic Factors / Q000302:isolation & purification / Q000378:metabolism*; D006098:Granulocytes / Q000378:metabolism*; D007249:Inflammation / Q000209:etiology; D016209:Interleukin-8; D007378:Interleukins / Q000302:isolation & purification / Q000378:metabolism*; D009504:Neutrophils / Q000378:metabolism*; D011817:Rabbits; D012709:Serum Albumin / Q000378:metabolism; D012867:Skin / Q000378:metabolism*
D002630:Chemotactic Factors; D016209:Interleukin-8; D007378:Interleukins; D012709:Serum Albumin