title: Excitatory amino acids interfere with normal eye growth in posthatch chick.
abstract: This study examines the effects of excitotoxic amino acids on eye growth and retinal morphology. Day old chicks received a single intraocular injection of either 200 nmoles kainic acid (KA), 200 nmoles quisqualic acid (QUIS) or 400 nmoles N-methyl-D,L-aspartate (NMDA). Following survival periods of 7, 14 and 21 days, eyeballs were removed and weighed. Measurements of axial length, equatorial length, anterior chamber depth and corneal diameter were taken. Treatment with KA increased eye weight and equatorial length. Treatment with QUIS increased the anterior chamber depth but decreased the equatorial length. Treatment with NMDA increased anterior chamber depth, but to a lesser extent than QUIS. The effects of QUIS and NMDA could be distinguished from those of KA since the former excitotoxins resulted in a marked increase in anterior chamber depth with no enlargement of vitreal chamber. Changes in eye size were evident by day 7 and were sustained throughout the duration of the experiment. Examination of retinae revealed that KA lesions amacrine cells, bipolar cells, some ganglion cells and photoreceptors. Exposure to QUIS lesions amacrine cells, horizontal cells and causes mild disruption of photoreceptor outer segments. In contrast, NMDA predominantly lesions amacrine cells. The results demonstrate that these neurotoxins have different effects on eye growth, which may be associated with differences in retinal pathology. It is proposed that photoreceptors are ideally suited to play a role in the control of eye growth.
authors.affiliation: Department of Anatomy, Monash University, Clayton, Victoria, Australia.
authors.forename: M
authors.initials: M
authors.lastname: Barrington
authors.forename: J
authors.initials: J
authors.lastname: Sattayasai
authors.forename: J
authors.initials: J
authors.lastname: Zappia
authors.forename: D
authors.initials: D
authors.lastname: Ehrlich
mesh_terms: D000818:Animals; D000867:Anterior Chamber / Q000033:anatomy & histology; D001224:Aspartic Acid / Q000494:pharmacology; D002645:Chickens; D005123:Eye / Q000187:drug effects*; D007608:Kainic Acid / Q000494:pharmacology*; D016202:N-Methylaspartate; D009929:Organ Size; D010069:Oxadiazoles / Q000494:pharmacology*; D016318:Quisqualic Acid; D012160:Retina / Q000473:pathology; D013997:Time Factors
chemical_list: D010069:Oxadiazoles; D001224:Aspartic Acid; D016202:N-Methylaspartate; D016318:Quisqualic Acid; D007608:Kainic Acid