Glycine potentiates the NMDA-induced release of dopamine through a strychnine-insensitive site in the rat striatum.
A new procedure, involving a push-pull cannula, was used to estimate the release of [3H]dopamine ([3H]DA) synthesized from [3H]tyrosine in rat striatal slices. NMDA (5 x 10(-5) M) stimulated [3H]DA release in the absence of Mg2+, and this effect was abolished in the presence of Mg2+ (10(-3) M), MK-801 (10(-6) M) or kynurenate (10(-4) M). Glycine markedly potentiated the NMDA-evoked response and reversed the inhibitory effect of kynurenate in the absence of Mg2+ and in the presence of strychnine (10(-6) M).
Krebs
M O
MO
Collège de France, INSERM U 114, Paris, France.
Kemel
M L
ML
Gauchy
C
C
Desban
M
M
Glowinski
J
J
D000818:Animals; D001224:Aspartic Acid / Q000494:pharmacology; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D003986:Dibenzocycloheptenes / Q000494:pharmacology; D016291:Dizocilpine Maleate; D004298:Dopamine / Q000378:metabolism*; D004357:Drug Synergism; D005998:Glycine / Q000494:pharmacology*; D066298:In Vitro Techniques; D007736:Kynurenic Acid / Q000494:pharmacology; D008297:Male; D016202:N-Methylaspartate; D051381:Rats; D011919:Rats, Inbred Strains; D013331:Strychnine / Q000494:pharmacology*
D003986:Dibenzocycloheptenes; D001224:Aspartic Acid; D016202:N-Methylaspartate; D016291:Dizocilpine Maleate; D007736:Kynurenic Acid; D013331:Strychnine; D005998:Glycine; D004298:Dopamine