title: Characterization of Japanese encephalitis virus envelope protein expressed by recombinant baculoviruses.
abstract: Recombinant baculoviruses containing the coding sequences of the viral structural proteins, i.e., the capsid (C) protein, the precursor to premembrane (preM) protein, and the envelope (E) protein, as well as a nonstructural protein, NS1, of Japanese encephalitis virus (JEV) were constructed. Infection of Spodoptera frugiperda cells with these recombinant viruses produced PreM and E proteins. The E proteins synthesized by the recombinants were shown to be glycosylated and similar in size to the authentic E protein. The E protein was found on the surface of infected cells. The antigenic properties of recombinant E proteins were evaluated using a panel of monoclonal antibodies produced against JEV E protein. It was demonstrated that all of the epitopes detectable on the authentic JEV E protein were present on the recombinant E protein expressed by a recombinant baculovirus containing the coding sequence for a part of C, PreM, E, and a part of NS1 proteins. However, for E protein expressed by a recombinant baculovirus having the coding sequence of only a part of PreM, but all of E and a part of NS1, one of the flavivirus cross-reactive epitopes was not detected. Mice immunized with cells infected with the recombinant baculoviruses developed neutralization antibodies.
authors.affiliation: Department of Veterinary Science, National Institute of Health, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuura
authors.forename: M
authors.initials: M
authors.lastname: Miyamoto
authors.forename: T
authors.initials: T
authors.lastname: Sato
authors.forename: C
authors.initials: C
authors.lastname: Morita
authors.forename: K
authors.initials: K
authors.lastname: Yasui
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000276:immunology; D015139:Blotting, Southern; D015153:Blotting, Western; D002478:Cells, Cultured; D004279:DNA, Viral / Q000235:genetics; D004664:Encephalitis Virus, Japanese / Q000235:genetics* / Q000276:immunology; D015967:Gene Expression Regulation, Viral; D005822:Genetic Vectors; D006385:Hemagglutination Inhibition Tests; D007304:Insect Viruses / Q000235:genetics; D007313:Insecta; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D014162:Transfection; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology; D014764:Viral Proteins / Q000096:biosynthesis / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D014759:Viral Envelope Proteins; D014764:Viral Proteins