Suppression of human lymphocyte chemotaxis and transendothelial migration by anti-LFA-1 antibody.
The role of lymphocyte function-associated antigen 1 (LFA-1) in human T cell chemotaxis was investigated by using mAb specific to the beta-chain (TS1/18) (CD18) and alpha-chain (TS1/22) (CD11a) of LFA-1. T cell chemotaxis in response to IL-2 and to lymphocyte chemotactic factor (LCF) was markedly suppressed by the addition of TS1/18. TS1/22 was a less effective inhibitor than TS1/18 with only LCF stimulated responses showing significant inhibition when compared in seven different T cell preparations. Neither TS1/18 nor TS1/22 antibody inhibited random T cell migration. Control mAb to CD4 T cells failed to inhibit T cell random migration or chemotaxis. Additional studies to evaluate the adherence and migration of T cells through IL-1-stimulated human umbilical vein endothelial cell (HUVEC) monolayers showed that both TS1/22 and TS1/18 suppressed T cell migration through HUVEC, but failed to inhibit adherence of T cells to these cells. These studies indicate that LFA-1 plays a role in the migration of T cells through HUVEC and in the in vitro chemotactic response of T lymphocytes to IL-2 and LCF.
Van Epps
D E
DE
Applied Sciences, Baxter Healthcare Corporation, Round Lake, IL 60073.
Potter
J
J
Vachula
M
M
Smith
C W
CW
Anderson
D C
DC
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D000943:Antigens, Differentiation / Q000276:immunology*; D002465:Cell Movement / Q000187:drug effects; D019744:Chemokines, C; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D004730:Endothelium, Vascular / Q000276:immunology / Q000502:physiology*; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007376:Interleukin-2 / Q000494:pharmacology; D016169:Lymphocyte Function-Associated Antigen-1; D008222:Lymphokines / Q000494:pharmacology; D051379:Mice; D016028:Receptors, Leukocyte-Adhesion / Q000276:immunology*; D012795:Sialoglycoproteins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000502:physiology*
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D019744:Chemokines, C; D007166:Immunosuppressive Agents; D007376:Interleukin-2; D016169:Lymphocyte Function-Associated Antigen-1; D008222:Lymphokines; D016028:Receptors, Leukocyte-Adhesion; D012795:Sialoglycoproteins; C092390:XCL1 protein, human; C486249:Xcl1 protein, mouse; C018997:lymphotactin