In vitro growth properties and PCR ras gene analysis of a murine embryonal carcinoma cell line harboring an amplified c-Ki-ras gene.
Ras proto-oncogenes are thought to be involved in both proliferation and malignant processes. We examined some growth properties of a murine embryonal carcinoma cell line (ECC), PCC4, that have been shown previously to be amplified for the c-KI-ras gene. Our results show that doubling time and plating efficiency are not significantly affected by such amplification. To examine the possible link between malignant behavior and c-Ki-ras alteration, we investigated activating mutations in this PCC4 cell line as well as in other ECC. Analysis of the in vitro amplified Ki-ras gene by PCR technology has not revealed point mutations in any of the ECC examined.
Vilette
D
D
UPR 43 CNRS Rétrovirus et Rétrotransposons des Vertébrés.
d'Auriol
L
L
Galibert
F
F
Peries
J
J
Emanoil-Ravier
R
R
D000818:Animals; D002277:Carcinoma; D002455:Cell Division; D002460:Cell Line; D005784:Gene Amplification; D011905:Genes, ras; D051379:Mice; D009154:Mutation; D011519:Proto-Oncogenes; D014407:Tumor Cells, Cultured / Q000166:cytology*