title: Two nuclear oncogenic proteins, P135gag-myb-ets and p61/63myc, cooperate to induce transformation of chicken neuroretina cells.
abstract: Several studies have shown that full transformation of primary rodent fibroblasts can be achieved in vitro through the cooperation of two oncogenes (usually one nuclear and one cytoplasmic) classified on the basis of different complementation groups. We have shown previously that cooperation between v-mil (cytoplasmic, serine-threonine kinase product), and v-myc (nuclear, DNA-binding product) is required to transform 7-day-old chicken neuroretina cells, which in usual culture medium do not rapidly proliferate. v-mil induces sustained growth of chicken neuroretina cells without transformation; v-myc fails to stimulate the proliferation of chicken neuroretina cells but is required to achieve transformation of the proliferating cells. Here, we present results indicating that the P135gag-myb-ets nuclear protein of avian erythroblastosis virus E26 is able to induce proliferation but not transformation of chicken neuroretina cells. v-myc is required in addition to P135gag-myb-ets to achieve chicken neuroretina cell transformation. In contrast, we found that the P135gag-myb-ets and P100gag-mil proteins are not able to cooperate in this system.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, U 186, Centre National de la Recherche Scientifique, URA 0156, Institut Pasteur de Lille, France.
authors.forename: P
authors.initials: P
authors.lastname: Amouyel
authors.forename: V
authors.initials: V
authors.lastname: Laudet
authors.forename: P
authors.initials: P
authors.lastname: Martin
authors.forename: R P
authors.initials: RP
authors.lastname: Li
authors.forename: B
authors.initials: B
authors.lastname: Quatannens
authors.forename: D
authors.initials: D
authors.lastname: Stéhelin
authors.forename: S
authors.initials: S
authors.lastname: Saule
mesh_terms: D000818:Animals; D002455:Cell Division; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002642:Chick Embryo; D003001:Cloning, Molecular; D005455:Fluorescent Antibody Technique; D015683:Gene Products, gag; D016274:Oncogene Protein p55(v-myc); D020626:Oncogene Proteins v-myb; D009856:Oncogene Proteins, Viral / Q000502:physiology*; D009857:Oncogenes; D011533:Proviruses / Q000235:genetics*; D012160:Retina; D012190:Retroviridae / Q000235:genetics*; D012191:Retroviridae Proteins / Q000502:physiology; D015682:Retroviridae Proteins, Oncogenic
chemical_list: D015683:Gene Products, gag; D016274:Oncogene Protein p55(v-myc); D020626:Oncogene Proteins v-myb; D009856:Oncogene Proteins, Viral; D012191:Retroviridae Proteins; D015682:Retroviridae Proteins, Oncogenic; C050379:oncogene proteins v-ets