Transforming growth factor-beta blocks proliferation but not early mitogenic signaling events in T-lymphocytes.
Transforming growth factor-beta (TGF-beta) inhibits the proliferation of T-lymphocytes in response to activation with mitogenic lectin. The influence of TGF-beta on elevation of cytosolic Ca2+, induction of proliferation-associated mRNA species, and total cellular RNA content has been studied. The cells seem to exit G0 when activated in the presence of TGF-beta, but they arrest in mid-G1 phase.
Morris
D R
DR
Department of Biochemistry, University of Washington, Seattle 98195.
Kuepfer
C A
CA
Ellingsworth
L R
LR
Ogawa
Y
Y
Rabinovitch
P S
PS
D000165:Acridine Orange; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D005434:Flow Cytometry / Q000379:methods; D005456:Fluorescent Dyes; D005796:Genes / Q000187:drug effects; D006657:Histones / Q000235:genetics; D007211:Indoles; D008213:Lymphocyte Activation / Q000187:drug effects*; D009955:Ornithine Decarboxylase / Q000235:genetics; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics; D016271:Proto-Oncogene Proteins c-myc; D011519:Proto-Oncogenes / Q000187:drug effects; D012333:RNA, Messenger / Q000032:analysis; D015398:Signal Transduction / Q000187:drug effects; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D015291:Transforming Growth Factors / Q000494:pharmacology*
D005456:Fluorescent Dyes; D006657:Histones; D007211:Indoles; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D015291:Transforming Growth Factors; D011505:Protein-Tyrosine Kinases; D009955:Ornithine Decarboxylase; D000165:Acridine Orange; C048960:indo-1; D002118:Calcium