Phenotypic characterization of mononuclear white cells using finger-prick blood and light microscopy.
A technique for the enumeration of T cell subsets in capillary blood for use in the field is described. Mononuclear white cells were separated from 0.4 ml capillary blood, kept covered by culture medium on a Multitest slide, and incubated with monoclonal antibodies to the CD2, CD4, CD8, and interleukin-2 receptor antigens. Secondary and tertiary alkaline phosphatase conjugates were used for labeling. The substrate gave a distinct blue surface staining to the positive lymphocytes. Comparison was made with a conventional immunofluorescent antibody technique (r = 0.95).
Smedman
L
L
University of Stockholm, Sweden.
Troye-Blomberg
M
M
Perlmann
H
H
Naucler
A
A
Perlmann
P
P
D000328:Adult; D001402:B-Lymphocytes / Q000166:cytology; D001815:Bloodletting / Q000379:methods*; D002675:Child, Preschool; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D007958:Leukocyte Count / Q000379:methods; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D010641:Phenotype; D013601:T-Lymphocytes / Q000145:classification / Q000166:cytology