Unexpected loss of immunoreactivity of a glucose oxidase-labeled antigen in a steroid enzyme immunoassay system.
Glucose oxidase-labeled 11-deoxycortisol was tested for immunoreactivity in an enzyme immunoassay system using a monoclonal antibody. The antigen was labeled by the N-succinimidyl ester method. It was found that the binding affinity of the label to the anti-steroid antibody markedly decreased during storage; the half-life time was ca. 4.5 d.
Hosoda
H
H
Itoh
M
M
Tsukamoto
R
R
Nambara
T
T
D000818:Animals; D000941:Antigens / Q000276:immunology*; D005949:Glucose Oxidase / Q000276:immunology*; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D013256:Steroids / Q000276:immunology*
D000941:Antigens; D013256:Steroids; D005949:Glucose Oxidase