title: [A fermenter dialysis cultivation technic for encapsulated pathogenic bacteria. II. Group B streptococci].
abstract: For mass cultivation of group B streptococci (B-III and B-variant streptococci), a fermenter dialysis culture technique is described and compared to conventional shaking culture and fermenter batch culture techniques. The influence of two kinds of cultivation media on the bacterial yield is demonstrated. The growth rate of the bacteria and the yield of the microbes is higher in modified POPE medium than that observed with Nährbouillon I. The type-specific polysaccharide of B-III-streptococci prepared by phenol-water extraction followed by gel-chromatography can be used as a screening antigen for the production of monoclonal antibodies against B-III-streptococci.
authors.affiliation: Institut für Medizinische Mikrobiologie des Bereiches Medizin, Charite, der Humboldt-Universität zu Berlin.
authors.forename: R
authors.initials: R
authors.lastname: Starke
authors.forename: M
authors.initials: M
authors.lastname: Mehl
authors.forename: C
authors.initials: C
authors.lastname: Möckel
authors.forename: R
authors.initials: R
authors.lastname: Grunow
authors.forename: T
authors.initials: T
authors.lastname: Montag
authors.forename: R
authors.initials: R
authors.lastname: Von Baehr
authors.forename: G
authors.initials: G
authors.lastname: Schmidt
mesh_terms: D000942:Antigens, Bacterial / Q000276:immunology / Q000302:isolation & purification; D003470:Culture Media; D003956:Dialysis; D005285:Fermentation; D013292:Streptococcus agalactiae / Q000254:growth & development* / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial; D003470:Culture Media
vernacular_title: Einsatz der Fermenter-Dialyse-Kultur zur Züchtung kapseltragender Bakterien mit klinischer Bedeutung.