title: Evaluation of the Rapid Strep system for identification of gram-positive, catalase-negative cocci isolated from bovine intramammary infections.
abstract: The Rapid Strep system (Analytab Products, Plainview, NY) was used to identify 199 gram-positive, catalase-negative cocci isolated from bovine intramammary infections. The system accurately identified 88.4% of isolates. The system identified 100% of 46 Streptococcus agalactiae, 100% of 48 Streptococcus dysgalactiae, 54.5% of 11 Streptococcus equinus, and 96.2% of 53 Streptococcus uberis isolates. Enterococcus spp. were identified correctly 83.3% of the time. One of 4 Streptococcus saccharolyticus strains was identified as Streptococcus bovis, the previous classification for this organism, and 8 Streptococcus equi ssp. equi strains were misidentified as Streptococcus dysgalactiae. The Rapid Strep system was determined to be an acceptable alternative to conventional methods for identification of gram-positive, catalase-negative cocci isolated from bovine intramammary infections.
authors.affiliation: Mastitis Research Laboratory, Louisiana State University Agricultural Center, Homer 71040.
authors.forename: J L
authors.initials: JL
authors.lastname: Watts
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques; D002374:Catalase / Q000378:metabolism; D002417:Cattle; D005260:Female; D008414:Mastitis, Bovine / Q000382:microbiology*; D011237:Predictive Value of Tests; D013290:Streptococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013291:Streptococcus / Q000145:classification* / Q000201:enzymology / Q000302:isolation & purification; D013292:Streptococcus agalactiae / Q000145:classification / Q000302:isolation & purification
chemical_list: D002374:Catalase