Growth properties of human herpesvirus-6 strain Z29.
Experiments performed to optimize the growth conditions of HHV-6(Z29) revealed that the virus grows best in phytohemagglutinin-stimulated umbilical cord blood lymphocytes (CBL) cultured in media containing 32 units/ml interleukin-2 and 0.01 mg/ml hydrocortisone. The titer of maternal antibody in the plasma of the cord blood cells does not affect the ability of the cells to support virus growth. DEAE-dextran and polybrene do not increase virus growth in umbilical cord blood lymphocytes. Phorbol myristate acetate abolishes virus growth. The HHV-6(Z29) growth cycle in CBL was approximately 5 days; capsids were not seen before day 3, and mature virions were not seen before day 5.
Black
J B
JB
Department of Experimental Pathology, Emory University, Atlanta, GA 30322.
Sanderlin
K C
KC
Goldsmith
C S
CS
Gary
H E
HE
Lopez
C
C
Pellett
P E
PE
D002478:Cells, Cultured; D003470:Culture Media; D004279:DNA, Viral / Q000032:analysis; D005312:Fetal Blood; D005455:Fluorescent Antibody Technique; D006564:Herpesviridae / Q000187:drug effects / Q000235:genetics / Q000254:growth & development* / Q000648:ultrastructure; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology; D008214:Lymphocytes; D010835:Phytohemagglutinins / Q000494:pharmacology; D014779:Virus Replication
D003470:Culture Media; D004279:DNA, Viral; D007376:Interleukin-2; D010835:Phytohemagglutinins; D006854:Hydrocortisone