Immunohistochemical demonstration of the asialoglycoprotein receptor in rat liver by a sensitive avidin-biotin technique.
An immunohistochemical technique for the detection of the hepatic asialoglycoprotein receptor (ASGP-R) in cryostat sections of liver by polyclonal and monoclonal anti-ASGP-R antibodies is described. The procedure is based on the alkaline-phosphatase-avidin-biotin complex (ABC-AP) system and important features include fixation of the sections with periodate-lysine-paraformaldehyde (with or without dichromate) and an absolute requirement for blocking of endogenous biotin activity. The sensitivity of the technique is such that binding to ASGP-R can be detected with femtomolar concentrations of monoclonal anti-ASGP-R antibodies and, with polyclonal antisera, approaches that of a radioimmunoassay.
Sipos
J
J
Liver Unit, King's College Hospital, Denmark Hill, London, U.K.
McFarlane
B M
BM
McSorley
C G
CG
Gove
C D
CD
Williams
R
R
McFarlane
I G
IG
D000818:Animals; D000906:Antibodies; D037263:Asialoglycoprotein Receptor; D001360:Avidin / Q000378:metabolism; D001710:Biotin / Q000378:metabolism; D007124:Immunoenzyme Techniques; D007202:Indicators and Reagents / Q000378:metabolism; D008099:Liver / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D011971:Receptors, Immunologic / Q000032:analysis*
D000906:Antibodies; D037263:Asialoglycoprotein Receptor; D007202:Indicators and Reagents; D011971:Receptors, Immunologic; D001360:Avidin; D001710:Biotin