Correlation between apolipoprotein A-IV and triglyceride concentrations in human sera.
Apolipoprotein A-IV concentration was measured by a newly developed competitive enzyme immunoassay in sera from fasted human subjects (n = 105) whose triglyceride concentrations ranged from 20 to 474 mg/dl (total cholesterol below 260 mg/dl) and in which chylomicrons could not be detected. Mean (+/- SD) apolipoprotein A-IV concentration was 13.0 +/- 2.6 mg/dl in sera with triglyceride levels ranging from 20 to 100 mg/dl, 16.9 +/- 3.7 mg/dl in sera with triglyceride levels ranging from 101 to 250 mg/dl, and 22.7 +/- 6.7 mg/dl in sera with triglyceride levels ranging from 251 to 474 mg/dl. The differences among the three groups were highly significant (P less than 0.001). Moreover, variations of apolipoprotein A-IV concentrations according to the triglyceride levels were noted within the normo-triglyceridemic population. Apolipoprotein A-IV concentration was 12.8 +/- 2.1 mg/dl for triglyceride levels ranging from 20 to 75 mg/dl and 16.4 +/- 3.8 mg/dl for triglyceride levels ranging from 76 to 150 mg/dl (P less than 0.01). In the entire population that was studied there was a significant linear correlation (r = 0.61, P less than 0.001) between the concentrations of serum apolipoprotein A-IV and triglyceride. Although the hypothesis of an unknown factor independently influencing both very low density lipoproteins and apolipoprotein A-IV cannot be ruled out, and although no apolipoprotein A-IV was found in the triglyceride-rich lipoprotein fraction after separation by gel filtration, these data suggest that, in fasting subjects, the secretion of very low density lipoproteins could contribute to the plasma apolipoprotein A-IV level.
Lagrost
L
L
Laboratoire de Biochimie Médicale, Faculté de Médecine, Dijon, France.
Gambert
P
P
Meunier
S
S
Morgado
P
P
Desgres
J
J
d'Athis
P
P
Lallemant
C
C
D000328:Adult; D001054:Apolipoproteins A / Q000097:blood*; D005260:Female; D006801:Humans; D015228:Hypertriglyceridemia / Q000097:blood; D007124:Immunoenzyme Techniques; D008297:Male; D012016:Reference Values; D014280:Triglycerides / Q000097:blood*
D001054:Apolipoproteins A; D014280:Triglycerides; C022205:apolipoprotein A-IV