Immunodetection of haemocyte subpopulations by N-acetylmuramic acid antibody in Planorbarius corneus (L.) (Gastropoda, Pulmonata).
The cell subpopulations in the haemolymph of Planorbarius corneus were distinguished by means of flow cytometry. An antibody against N-acetylmuramic acid was prepared and used as a cellular marker to recognize the cell types forming the subpopulations. The spreading haemocytes showed a positive reaction for anti-N-acetylmuramic acid; round haemocytes gave a negative reaction.
Ottaviani
E
E
Department of Animal Biology, University of Modena, Italy.
Montagnani
G
G
D000818:Animals; D000911:Antibodies, Monoclonal; D001773:Blood Cells / Q000032:analysis*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006434:Hemocytes / Q000032:analysis*; D008974:Mollusca / Q000032:analysis / Q000166:cytology*; D009112:Muramic Acids / Q000276:immunology
D000911:Antibodies, Monoclonal; D009112:Muramic Acids; C031651:N-acetylmuramic acid