Determination of peritoneal glucose kinetics in rats: implications for the peritoneal implantation of closed-loop insulin delivery systems.
Peritoneal glucose kinetics were evaluated in the anaesthetized rat, to assess whether the peritoneal cavity would be a suitable site for the implantation of membrane-protected islets of Langerhans (bioartificial pancreas) or the glucose sensor of an artificial B cell. Glucose was measured in peritoneal fluid samples aspirated by needle puncture. Basal peritoneal and blood glucose concentrations were identical in 16 h fasted (n = 4) and non fasted (n = 3) animals. After 10 min of an i.v. glucose infusion (n = 15) the increment in peritoneal glucose concentration was 63 +/- 3% of the increment in blood glucose concentration and both values were significantly correlated (r = 0.92; p less than 0.001). After 10 min of glucose clamping (12.6 +/- 0.8 mmol/l), the increment in peritoneal glucose concentration was 69 +/- 3% (n = 5; p less than 0.05) of the increment in blood glucose concentration. In three additional experiments it was 93 +/- 3% of the increment in blood glucose concentration (NS), after 30 min of glucose clamping (8.0 +/- 0.5 mmol/l). Peritoneal glucose concentration monitored by a glucose sensor: (a) followed blood glucose sluggishly during a glucose clamp (n = 5), confirming the data shown above, (b) followed blood glucose with a 5 min delay and reached the same plateau after the intravenous injection of 1U insulin (n = 3; NS). We conclude that peritoneal glucose reflects blood glucose at basal state and during variations of glycaemia, nevertheless, presenting heterogeneous kinetics. These kinetics might be appropriate for a bioartificial pancreas but not for an in vivo calibration procedure, of a peritoneally implanted glucose sensor.
Velho
G
G
Service de Diabétologie, Hôtel-Dieu, Paris, France.
Froguel
P
P
Reach
G
G
D000818:Animals; D015374:Biosensing Techniques; D001786:Blood Glucose / Q000378:metabolism; D005947:Glucose / Q000008:administration & dosage / Q000032:analysis / Q000378:metabolism*; D015309:Glucose Clamp Technique; D007262:Infusions, Intravenous; D007328:Insulin / Q000494:pharmacology; D007332:Insulin Infusion Systems; D007700:Kinetics; D008297:Male; D010529:Peritoneal Cavity / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D001786:Blood Glucose; D007328:Insulin; D005947:Glucose