Comparative analysis of the Salmonella typhi and Escherichia coli ompC genes.
The nucleotide (nt) sequence of the gene encoding the Salmonella typhi OmpC outer membrane protein, and its deduced amino acid (aa) sequence are presented here. The S. typhi ompC gene consists of an open reading frame of 1134 nt, corresponding to a protein of 378 aa; with a 21-aa signal peptide. This protein is 11 aa longer than Escherichia coli OmpC, but it has an identical leader peptide. The mature OmpC sequence shows 79% similarity for both bacteria at the aa level, and 77% similarity at the nt level. Seven main variable regions in the OmpC protein were identified. Five of them correspond to hydrophilic regions and contain aa observed most frequently in turn configurations in soluble proteins. This suggests that these aa stretches could be located on the exterior of the outer membrane. To probe into the genus and species specificity of the main variable regions, we have constructed complementary oligodeoxyribonucleotides. The use of one of them with a small number of DNA samples is illustrated here; no restriction fragment length polymorphism or nt sequence heterogeneity could be found between S. typhi and Salmonella typhimurium.
Puente
J L
JL
Departamento de Biología Molecular, Universidad Nacional Autónoma de México.
Alvarez-Scherer
V
V
Gosset
G
G
Calva
E
E
D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001483:Base Sequence; D003062:Codon / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D011487:Protein Conformation; D015183:Restriction Mapping; D012485:Salmonella typhi / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D001425:Bacterial Outer Membrane Proteins; D003062:Codon