Expression in Escherichia coli of catalytically active phenylalanine ammonia-lyase from parsley.
In parsley (Petroselinum crispum), phenylalanine ammonia-lyase (PAL) is encoded by 4 structurally similar genes. The nucleotide sequence of a near full-length cDNA and the deduced amino acid sequence of PAL-4 are presented and compared with the corresponding sequences of PAL-1, a previously described representative of the gene family. Transformation of Escherichia coli cells with PAL-1 or PAL-4 cDNA yielded catalytically active PAL, suggesting that the catalytic center of the enzyme is formed spontaneously rather than by a plant-specific mechanism.
Schulz
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Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Kln, FRG.
Eiben
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Hahlbrock
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D000642:Ammonia-Lyases / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010650:Phenylalanine Ammonia-Lyase / Q000235:genetics* / Q000378:metabolism; D010944:Plants / Q000201:enzymology / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D015345:Oligonucleotide Probes; D000642:Ammonia-Lyases; D010650:Phenylalanine Ammonia-Lyase