[Advanced research in hypoxic cells].
Novel methodology and ideas which have recently been developed in the world were shortly reviewed in the field of tumor hypoxia. At subcellular level, hypoxia induces specific protein while hypoxia inhibits initiation of DNA replication. Radioresistant characteristics of hypoxic cells are questioned under two condition, i.e., post-irradiation hypoxia and chronic hypoxia in multicellular spheroids. A novel sandwich culture system can differentiate between oxic and chronic hypoxia with oxygen effect being excluded. Evidence for perfusion-limited, acute hypoxia is provided by a novel method employing vital staining and cell sorter.
Ando
K
K
Division of Clinical Research, National Institute of Radiological Sciences.
D000818:Animals; D015687:Cell Hypoxia / Q000528:radiation effects*; D002470:Cell Survival / Q000528:radiation effects; D046508:Culture Techniques / Q000379:methods; D004261:DNA Replication; D006801:Humans; D051379:Mice; D009374:Neoplasms, Experimental / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D014176:Protein Biosynthesis; D011836:Radiation Tolerance; D014407:Tumor Cells, Cultured / Q000378:metabolism* / Q000528:radiation effects