Fluoroimmunoassay studies with solubilized antigens from Borrelia burgdorferi.
Sodium deoxycholate-solubilized Borrelia burgdorferi antigen was prepared for use in a solid-phase fluoroimmunoassay (FIA-L) to detect antibodies in Lyme disease. Serum specimens were tested by FIA-L and by a microimmunofluorescence test. The FIA-L results are comparable to those of the standard microimmunofluorescence test. The overall agreement was 0.98. Moreover, the FIA-L procedure is simple and rapid; fluorescence is objectively determined and is proportional to antibody titer.
Hechemy
K E
KE
Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509.
Harris
H L
HL
Wethers
J A
JA
Stevens
R W
RW
Stock
B R
BR
Reilly
A A
AA
Benach
J L
JL
D000907:Antibodies, Bacterial / Q000032:analysis*; D000942:Antigens, Bacterial / Q000276:immunology*; D001898:Borrelia / Q000276:immunology*; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D006801:Humans; D015151:Immunoblotting; D008193:Lyme Disease / Q000175:diagnosis*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial