The healing of chronic venous leg ulcers with prepared human amnion.
Forty chronic venous leg ulcers were treated, before split skin grafting, with human amnion prepared in one of the four following ways: tissue-culture-maintained, frozen, fresh or lyophilised. Although there was no significant statistical difference in the results obtained with the different preparations of amnion, we found that lyophilised amnion was at least as good as the other preparations in promoting a good take of the skin grafts and was the simplest to store and use. It also produced the shortest healing times. Frozen and fresh amnion were easier to prepare than lyophilised amnion but gave a lower graft take and a longer healing time. Tissue-culture-maintained amnion was the most difficult to prepare and gave the poorest results. Its use was abandoned during the trial because of technical difficulties and a high infection rate.
Ward
D J
DJ
Queen Victoria Hospital, East Grinstead.
Bennett
J P
JP
Burgos
H
H
Fabre
J
J
D000368:Aged; D000369:Aged, 80 and over; D000650:Amnion; D002908:Chronic Disease; D046508:Culture Techniques; D005260:Female; D005612:Freeze Drying; D005615:Freezing; D006801:Humans; D007871:Leg Ulcer / Q000601:surgery*; D008297:Male; D008875:Middle Aged; D016038:Skin Transplantation; D014021:Tissue Preservation