title: Nuclear magnetic resonance studies of 6-fluorotryptophan-substituted rat cellular retinol-binding protein II produced in Escherichia coli. Analysis of the apoprotein and the holoprotein containing bound all-trans-retinol and all-trans-retinal.
abstract: Rat cellular retinol-binding protein II (CRBP II) is a 15.6-kDa intestinal protein which binds all-trans-retinol and all-trans-retinal but not all-trans-retinoic acid. We have previously analyzed the interaction of Escherichia coli-derived rat apoCRBP II with several retinoids using fluorescence spectroscopic techniques. Interpretation of these experiments is complicated, because the protein has 4 tryptophan residues. To further investigate ligand-protein interactions, we have utilized 19F nuclear magnetic resonance (NMR) spectroscopy of CRBP II labeled at its 4 tryptophan residues with 6-fluorotryptophan. Efficient incorporation of 6-fluorotryptophan (93%) was achieved by growing a tryptophan auxotroph of E. coli harboring a prokaryotic expression vector with a full-length rat CRBP II cDNA on defined medium supplemented with the analog. Comparison of the 19F NMR spectra of 6-fluorotryptophan-substituted CRBP II with and without bound all-trans-retinol revealed that resonances corresponding to 2 tryptophan residues (designated WA and WB) undergo large downfield changes in chemical shifts (2.0 and 0.5 ppm, respectively) associated with ligand binding. In contrast, 19F resonances corresponding to two other tryptophan residues (WC and WD) undergo only minor perturbations in chemical shifts. The 19F NMR spectra of 6-fluorotryptophan-substituted CRBP II complexed with all-trans-retinal and all-trans-retinol were very similar, suggesting that the interactions of these two ligands with the protein are similar. Molecular model building, based on the crystalline structures of two homologous proteins was used to predict the positions of the 4 tryptophan residues of CRBP II and to make tentative resonance assignments. The fact that ligand binding produced residue-specific changes in the chemical shifts of resonances in CRBP II suggests that NMR analysis of isotopically labeled retinoid-binding proteins expressed in E. coli will provide an alternate, albeit it complementary, approach to fluorescence spectroscopy for examining the structural consequences of their association with ligand.
authors.affiliation: Department of Medicine, Washington University, St. Louis, Missouri 63110.
authors.forename: E
authors.initials: E
authors.lastname: Li
authors.forename: S J
authors.initials: SJ
authors.lastname: Quian
authors.forename: L
authors.initials: L
authors.lastname: Nader
authors.forename: N C
authors.initials: NC
authors.lastname: Yang
authors.forename: A
authors.initials: A
authors.lastname: d'Avignon
authors.forename: J C
authors.initials: JC
authors.lastname: Sacchettini
authors.forename: J I
authors.initials: JI
authors.lastname: Gordon
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001059:Apoproteins; D004247:DNA / Q000235:genetics; D004926:Escherichia coli / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D012172:Retinaldehyde / Q000378:metabolism*; D012176:Retinoids / Q000378:metabolism*; D012177:Retinol-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D054840:Retinol-Binding Proteins, Cellular; D013050:Spectrometry, Fluorescence; D014364:Tryptophan / Q000031:analogs & derivatives; D014801:Vitamin A / Q000378:metabolism*
chemical_list: D001059:Apoproteins; C518461:Rbp2 protein, rat; D011994:Recombinant Proteins; D012176:Retinoids; D012177:Retinol-Binding Proteins; D054840:Retinol-Binding Proteins, Cellular; D014801:Vitamin A; C001244:6-fluorotryptophan; D014364:Tryptophan; D004247:DNA; D012172:Retinaldehyde