Establishment of radioimmunoassay for human islet amyloid polypeptide and its tissue content and plasma concentration.
Using a synthetic C- terminal tetradecapeptide of human islet amyloid polypeptide (IAPP), we prepared an antiserum for human IAPP [24-37] and established a highly sensitive radioimmunoassay (RIA) for human IAPP. Analyses of human pancreatic extract using reverse-phase high performance liquid chromatography coupled with the RIA revealed that the antiserum specifically detects human IAPP. The content of IAPP in the pancreas of two non-diabetic patients was 604.0 and 1447.7 pg/mg wet weight, and a small amount of IAPP-immunoreactivity was detected in the stomach, duodenum, and jejunum. The mean plasma concentration of IAPP in 10 normal individuals was 13.5 +/- 4.8 (SD) pg/ml. The RIA established in this study provides a useful tool to elucidate the physiological function of IAPP and its pathophysiological significance in non-insulin-dependent diabetes mellitus (NIDDM).
Nakazato
M
M
Third Department of Internal Medicine, Miyazaki Medical College, Japan.
Asai
J
J
Kangawa
K
K
Matsukura
S
S
Matsuo
H
H
D000328:Adult; D000682:Amyloid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D058228:Islet Amyloid Polypeptide; D007515:Islets of Langerhans / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D011863:Radioimmunoassay; D014018:Tissue Distribution
D000682:Amyloid; D058228:Islet Amyloid Polypeptide