Pulsed field electrophoresis in contour-clamped homogeneous electric fields for the resolution of DNA by size or topology.
The electrophoretic separation of DNA molecules can be controlled by the use of contour-clamped homogeneous electric fields (CHEF). This paper describes an improved CHEF apparatus with negligible distortion in the electric fields. When the electric field was periodically reoriented, DNA molecules up to 2 megabases were resolved in a highly uniform manner. Furthermore, when the field strength was changed with orientation, topological variations of conventional-sized DNA molecules were resolved.
Chu
G
G
Department of Medicine, Stanford University, CA 94305.
D004247:DNA / Q000032:analysis*; D004271:DNA, Fungal / Q000032:analysis; D004278:DNA, Superhelical / Q000032:analysis; D004563:Electrochemistry; D004566:Electrodes; D004586:Electrophoresis / Q000295:instrumentation*; D004587:Electrophoresis, Agar Gel / Q000295:instrumentation* / Q000379:methods; D015394:Molecular Structure; D008970:Molecular Weight; D012441:Saccharomyces cerevisiae / Q000032:analysis
D004271:DNA, Fungal; D004278:DNA, Superhelical; D004247:DNA