Cloning of Salmonella typhimurium DNA encoding mutagenic DNA repair.
Mutagenic DNA repair in Escherichia coli is encoded by the umuDC operon. Salmonella typhimurium DNA which has homology with E. coli umuC and is able to complement E. coli umuC122::Tn5 and umuC36 mutations has been cloned. Complementation of umuD44 mutants and hybridization with E. coli umuD also occurred, but these activities were much weaker than with umuC. Restriction enzyme mapping indicated that the composition of the cloned fragment is different from the E. coli umuDC operon. Therefore, a umu-like function of S. typhimurium has been found; the phenotype of this function is weaker than that of its E. coli counterpart, which is consistent with the weak mutagenic response of S. typhimurium to UV compared with the response in E. coli.
Thomas
S M
SM
Genetics Division, National Institute for Medical Research, Mill Hill, London, England.
Sedgwick
S G
SG
D003001:Cloning, Molecular / Q000379:methods; D004260:DNA Repair; D004269:DNA, Bacterial / Q000235:genetics*; D004307:Dose-Response Relationship, Radiation; D004926:Escherichia coli / Q000235:genetics; D005838:Genotype; D009154:Mutation; D010957:Plasmids; D015183:Restriction Mapping; D012486:Salmonella typhimurium / Q000235:genetics* / Q000528:radiation effects; D014466:Ultraviolet Rays
D004269:DNA, Bacterial