title: Nuclear factors specifically bind to upstream sequences of a Xenopus laevis ribosomal protein gene promoter.
abstract: The upstream region of the Xenopus laevis L14 ribosomal protein gene was deleted starting from the 5' extremity in order to define the promoter length necessary to express a linked reporter CAT gene. The functional analysis indicated that a sequence located between -63 and -49 from the capsite is important for an efficient promoter activity. Band shift and ExoIII protection assays evidenced the binding to this region of a factor, called XrpFI, present in the crude nuclear extract from X.laevis oocytes. Methylation interference analysis localized the contacts in the G residues belonging to a short box, 5' CTTCC 3', positioned between -53 and -49 from the capsite. An additional factor, XrpFII, makes contacts with the sequence 5'GCCTGTTCGCC 3' located between -27 and -17 from the capsite. The deletion mutant still containing this sequence is poorly transcribed, but resumes activity when a short fragment containing the binding site for factor XrpFI is cloned in an upstream position.
authors.affiliation: Department of Genetics and Molecular Biology, University of Rome, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Carnevali
authors.forename: C
authors.initials: C
authors.lastname: La Porta
authors.forename: V
authors.initials: V
authors.lastname: Ilardi
authors.forename: E
authors.initials: E
authors.lastname: Beccari
mesh_terms: D000818:Animals; D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D002872:Chromosome Deletion; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005796:Genes; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000138:chemical synthesis; D009865:Oocytes / Q000201:enzymology; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012269:Ribosomal Proteins / Q000235:genetics*; D014158:Transcription, Genetic; D014982:Xenopus laevis
chemical_list: D004268:DNA-Binding Proteins; D015345:Oligonucleotide Probes; D012269:Ribosomal Proteins; C041174:ribosomal protein L14; D015500:Chloramphenicol O-Acetyltransferase