title: [Structural-functional analysis of the par-region of ColE1 plasmid].
abstract: The DNA fragment identical to the right shoulder of the inverted repeat from the par-region of ColE1 plasmid has been synthesized chemically. It is shown to participate in the plasmid multimers resolution and to define the stable inheritance of the plasmid pKS1 containing the fragment in Escherichia coli C600 cells as well as in the multirecombinogenic strain Escherichia coli JC8679. The efficiency of the fragments functioning in Escherichia coli JC8679 is not enough for resolution of all forms of oligomeric pKS1 DNA. The site for recombinase action is found to be located in the synthesized oligonucleotide. However, some extra sequences of DNA located within the region of inverted repeat are necessary for maximally efficient functioning of the recombinase, the enzyme participating in plasmid multimers resolution.
authors.forename: M N
authors.initials: MN
authors.lastname: Kolot
authors.forename: I I
authors.initials: II
authors.lastname: Shekhter
authors.forename: V P
authors.initials: VP
authors.lastname: Veĭko
authors.forename: K I
authors.initials: KI
authors.lastname: Ratmanova
authors.forename: M Z
authors.initials: MZ
authors.lastname: Gogniashvili
mesh_terms: D007446:Chromosome Inversion; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D004269:DNA, Bacterial
vernacular_title: Strukturno-funktsional'nyĭ analiz par-uchastka plazmidy ColE1.