A biotin-streptavidin amplified enzymeimmunoassay for 13,14-dihydro-15-keto-PGF2 alpha.
As an alternative for radioimmunoassays a new enzyme immunoassay (EIA) for the determination of 13,14-dihydro-15-keto PGF2 alpha (PGFM) has been developed. Biocytin was linked to PGFM by the N-hydroxysuccinimide method and the product (biocytinyl-PGFM) purified by reversed phase column chromatography. Biocytinyl-PGFM was used in the EIA as a bridge between the immobilized PGFM-antibody and streptavidin-peroxidase. The absolute sensitivity of the assay was about 160 amol (92% rel. binding) and required only 2 microliters plasma for PGFM estimation within the whole physiological range (0.08-20 pmol/ml). All variabilities were less than 14%. The described assay procedure may be of general applicability for other prostaglandins.
Meyer
H H
HH
Institut für Physiologie Südd, Technische Universität München, FRG.
Eisele
K
K
Osaso
J
J
D000818:Animals; D000906:Antibodies; D001360:Avidin; D001710:Biotin; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000097:blood / Q000276:immunology; D005260:Female; D005443:Flumethasone / Q000494:pharmacology; D007124:Immunoenzyme Techniques; D007743:Labor, Obstetric; D011247:Pregnancy; D011863:Radioimmunoassay; D012756:Sheep
D000906:Antibodies; D001360:Avidin; C008228:15-keto-13,14-dihydroprostaglandin F2alpha; D001710:Biotin; D015237:Dinoprost; D005443:Flumethasone