title: Membrane-binding sites for acyl carrier protein in Escherichia coli.
abstract: We report that membrane vesicles of Escherichia coli contain protein-binding sites for acyl carrier protein. Scatchard analysis of the binding indicates a dissociation constant around 0.35 micrometers and a maximum number of protein-binding sites around 50 pmol per mg of membrane protein. Binding is on the inner membrane while the outer membrane is devoid of binding sites. These results are consistent with the fact that some acyl carrier protein-dependent enzymes implicated in phospholipid- and membrane-derived oligosaccharide biosynthesis are localized in the cytoplasmic membrane.
authors.affiliation: Laboratoire de Biomembranes, Bât 433, Université Paris Sud, Orsay, France
authors.forename: N
authors.initials: N
authors.lastname: Bayan
authors.forename: H
authors.initials: H
authors.lastname: Therisod
mesh_terms: D000213:Acyl Carrier Protein / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism; D001665:Binding Sites; D002413:Cations, Divalent / Q000494:pharmacology; D002451:Cell Compartmentation; D002462:Cell Membrane / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism* / Q000648:ultrastructure; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007700:Kinetics; D008565:Membrane Proteins / Q000378:metabolism*
chemical_list: D000213:Acyl Carrier Protein; D001426:Bacterial Proteins; D002413:Cations, Divalent; D008565:Membrane Proteins