title: Detection of perforin and granzyme A mRNA in infiltrating cells during infection of mice with lymphocytic choriomeningitis virus.
abstract: The analysis of gene expression in cytotoxic T cells by in situ hybridization of serial liver and brain sections from mice infected with lymphocytic choriomeningitis virus (LCMV) and immunostaining with T cell marker- and virus-specific antibodies revealed a close histological association of infiltrating lymphocytes expressing the perforin and granzyme A genes with virally infected cells. Maximal frequency of perforin and granzyme A mRNA-containing cells on liver sections preceded by about 2 days maximal LCMV-specific cytotoxicity of the lymphoid liver infiltrating cells. These results are most consistent with an involvement of perforin and granzyme A in cell-mediated cytotoxicity in vivo.
authors.affiliation: Department of Pathology, University of Bern.
authors.forename: C
authors.initials: C
authors.lastname: Müller
authors.forename: D
authors.initials: D
authors.lastname: Kägi
authors.forename: T
authors.initials: T
authors.lastname: Aebischer
authors.forename: B
authors.initials: B
authors.lastname: Odermatt
authors.forename: W
authors.initials: W
authors.lastname: Held
authors.forename: E R
authors.initials: ER
authors.lastname: Podack
authors.forename: R M
authors.initials: RM
authors.lastname: Zinkernagel
authors.forename: H
authors.initials: H
authors.lastname: Hengartner
mesh_terms: D000818:Animals; D001921:Brain / Q000382:microbiology / Q000503:physiopathology; D005786:Gene Expression Regulation; D053804:Granzymes; D006524:Hepatitis, Viral, Animal / Q000235:genetics; D007124:Immunoenzyme Techniques; D008099:Liver / Q000382:microbiology / Q000473:pathology / Q000503:physiopathology; D008216:Lymphocytic Choriomeningitis / Q000382:microbiology / Q000473:pathology / Q000503:physiopathology*; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D009693:Nucleic Acid Hybridization; D054353:Perforin; D052899:Pore Forming Cytotoxic Proteins; D015347:RNA Probes; D012333:RNA, Messenger / Q000235:genetics; D012697:Serine Endopeptidases / Q000235:genetics*; D013602:T-Lymphocytes, Cytotoxic / Q000502:physiology*; D013997:Time Factors
chemical_list: D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D052899:Pore Forming Cytotoxic Proteins; D015347:RNA Probes; D012333:RNA, Messenger; D054353:Perforin; D053804:Granzymes; D012697:Serine Endopeptidases