title: Synaptonemal complex proteins.
abstract: Synaptonemal complexes were isolated from rate spermatocytes for the purpose of biochemical and morphological analysis. Several monoclonal antibodies were elicited against purified synaptonemal complexes to study the composition and assembly of these structures. Four classes of antibodies could be discriminated according to the polypeptides that they recognize on Western blots of purified synaptonemal complexes, namely antibodies recognizing (i) a 190-kDa polypeptide; (ii) a 30- and a 33-kDa polypeptide; (iii) two polypeptides with molecular weights of about 120 kDa; and (iv) polypeptides with molecular weights of 66-55 kDa. The localization of these antigens within spermatocytes was analyzed light microscopically, by means of the immunoperoxidase technique and ultrastructurally, by immunogold labelling of surface-spread spermatocytes. The 66- to 55-kDa polypeptides are not confined to synaptonemal complexes; rather, these polypeptides appear to be chromosomal components. The 190-, 30-, and 33-kDa polypeptides make part of the lateral elements of paired as well as unpaired segments of synaptonemal complexes. The 120-kDa polypeptides were localized on the inner edge of the lateral elements, specifically in paired segments of synaptonemal complexes. The distribution of the 190-, 120-, 30-, and 33-kDa polypeptides within the testis was analyzed by immunofluorescence staining of cryostat sections. All these polypeptides turned out to be specific for nuclei of zygotene up to and including diplotene spermatocytes. Only in some early spermatids could the 190-, 30-, and 33-kDa polypeptides be detected, presumably in remnants of synaptonemal complexes.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Institute of Human Genetics, University of Amsterdam, The Netherlands.
authors.forename: C
authors.initials: C
authors.lastname: Heyting
authors.forename: A J
authors.initials: AJ
authors.lastname: Dietrich
authors.forename: P B
authors.initials: PB
authors.lastname: Moens
authors.forename: R J
authors.initials: RJ
authors.lastname: Dettmers
authors.forename: H H
authors.initials: HH
authors.lastname: Offenberg
authors.forename: E J
authors.initials: EJ
authors.lastname: Redeker
authors.forename: A C
authors.initials: AC
authors.lastname: Vink
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008297:Male; D008540:Meiosis / Q000502:physiology*; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000302:isolation & purification* / Q000648:ultrastructure; D051381:Rats; D013090:Spermatocytes / Q000648:ultrastructure; D013573:Synaptonemal Complex / Q000276:immunology / Q000502:physiology*
chemical_list: D000911:Antibodies, Monoclonal; D009687:Nuclear Proteins