Level of satiety: in vitro energy metabolism in brain during hypophagic and hyperphagic body weight recovery.
Rates of in vitro glucose and fatty acid oxidation were examined in four brain sites during hypophagic and hyperphagic recovery of normal body weight. Rats were fed 40, 100, or 160% of normal intake, via gastric intubation, for 3 wk. Another group of rats was starved until body weight loss was equivalent to weight loss in 40%-fed rats. Groups of rats were killed at the conclusion of tube feeding or fasting and at specific periods during recovery of body weight. Brain sites examined were the ventrolateral hypothalamus (VLH), ventromedial hypothalamus (VMH), a caudal brain stem site encompassing the area postrema-nucleus of the solitary tract (AP-NTS), and cortex. During recovery, rats previously fed 160% of normal intake (anorectic) maintained low rates of VLH fatty acid oxidation and were hypophagic until most excess fat was depleted. Conversely, rats previously fed 40% of normal intake (hungry) maintained high rates of VLH fatty acid oxidation and were hyperphagic until most deficient fat was repleted. Rats previously starved maintained high rates of VLH fatty acid oxidation during hyperphagic recovery, although levels of VLH fatty acid oxidation and food intake were initially low on refeeding. Rates of glucose oxidation in the brain sites examined did not relate well to energy balance status and the needed adjustments in food intake. The results indicated that the level of glucose oxidation in the VLH and AP-NTS responded to the level of energy immediately coming into the system (food intake).(ABSTRACT TRUNCATED AT 250 WORDS)
Kasser
T R
TR
Department of Foods and Nutrition, University of Georgia, Athens 30602.
Harris
R B
RB
Martin
R J
RJ
D000818:Animals; D000855:Anorexia / Q000378:metabolism; D001835:Body Weight; D001921:Brain / Q000378:metabolism / Q000502:physiology*; D002250:Carbon Radioisotopes; D004734:Energy Metabolism; D005247:Feeding Behavior; D001068:Feeding and Eating Disorders / Q000503:physiopathology*; D005260:Female; D005947:Glucose / Q000378:metabolism; D006963:Hyperphagia / Q000503:physiopathology*; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D011865:Radioisotope Dilution Technique; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D012527:Satiation
D002250:Carbon Radioisotopes; D010169:Palmitic Acids; D019308:Palmitic Acid; D005947:Glucose