[An evaluation of the efficacy of immunoenzyme analysis with whole and fractionated antigens of Echinococcus granulosus].
Efficacy of EIA with intact (IA) and fractionated (FA) antigens on 34 lung echinococcosis patients' sera and 42 control sera samples has been evaluated. Sediment fraction SF-70, obtained from echinococcal fluid treated with 70% saturated ammonium sulphate, was the most effective, revealing specific antibodies in 97.2 +/- 2.9% of echinococcosis patients, while IA allowed to reveal them only in 82.4 +/- 6.5% of cases. The values in EIA diagnostic (1:200) titres were 76.5 +/- 7.3 and 54.1 +/- 4.0%, respectively. Both antigens specificity was high enough--95.2% for the intact and 97.6% for fractionated antigens.
Stepankovskaia
L P
LP
Shakarov
A G
AG
Kurbanov
A T
AT
D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis; D000947:Antigens, Helminth / Q000302:isolation & purification; D004443:Echinococcosis / Q000175:diagnosis; D004446:Echinococcus / Q000276:immunology*; D005069:Evaluation Studies as Topic; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007124:Immunoenzyme Techniques
D000909:Antibodies, Helminth; D000947:Antigens, Helminth
Otsenka éffektivnosti immunofermentnogo analiza s tsel'nym i fraktsionirovannymi antigenami Echinococcus granulosus.