title: Synthesis of human endothelin-1 precursors in Escherichia coli.
abstract: Endothelin, the most potent vasoconstrictor found in nature, is thought to be important in the regulation of blood pressure and/or local blood distribution. Human placenta cDNA fragment encoding preproendothelin-1 (preproET-1) and its carboxyl terminal mature precursor (C-matured precursor) was expressed in E. coli. These products were characterized by both enzyme immunoassay and Western blot analysis.
authors.affiliation: Tsukuba Research Laboratories, Takeda Chemical Industries, Ltd, Ibaraki, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
authors.forename: Y
authors.initials: Y
authors.lastname: Itoh
authors.forename: K
authors.initials: K
authors.lastname: Ogi
authors.forename: C
authors.initials: C
authors.lastname: Kimura
authors.forename: N
authors.initials: N
authors.lastname: Suzuki
authors.forename: H
authors.initials: H
authors.lastname: Onda
mesh_terms: D015153:Blotting, Western; D004247:DNA / Q000235:genetics*; D016232:Endothelins; D004730:Endothelium, Vascular; D004926:Escherichia coli / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D010452:Peptide Biosynthesis; D010455:Peptides / Q000235:genetics; D010957:Plasmids; D011498:Protein Precursors / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis*; D014158:Transcription, Genetic; D014169:Transformation, Bacterial
chemical_list: D016232:Endothelins; D010455:Peptides; D011498:Protein Precursors; D011994:Recombinant Proteins; D004247:DNA