title: Design of RNA enzymes for sequence-dependent cleavage of RNA.
abstract: We have designed synthetic ribozymes which can cleave specific sites of RNA. An undecamer, 5S rRNA and transcripts using T7 RNA polymerase were hydrolyzed at the X position of RNA containing (N)nUX(N)n (N = A, G, C and U; X = A, C and U) by these ribozymes.
authors.affiliation: Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Koizumi
authors.forename: Y
authors.initials: Y
authors.lastname: Hayase
authors.forename: S
authors.initials: S
authors.lastname: Iwai
authors.forename: H
authors.initials: H
authors.lastname: Kamiya
authors.forename: H
authors.initials: H
authors.lastname: Inoue
authors.forename: E
authors.initials: E
authors.lastname: Ohtsuka
mesh_terms: D001483:Base Sequence; D002384:Catalysis; D008969:Molecular Sequence Data; D009843:Oligoribonucleotides / Q000138:chemical synthesis / Q000378:metabolism*; D016337:RNA, Catalytic; D012335:RNA, Ribosomal / Q000378:metabolism*
chemical_list: D009843:Oligoribonucleotides; D016337:RNA, Catalytic; D012335:RNA, Ribosomal