Assignment of human desmin gene to band 2q35 by nonradioactive in situ hybridization.
A 3-kb DNA fragment, inserted in Bluescribe vector, was used to localize the desmin gene by in situ hybridization on human metaphase chromosomes. The probe was labelled by Bio-11-dUTP and detected by immunofluorescence. Subsequent R-banding indicated that the desmin gene is located in band 2q35.
Viegas-Péquignot
E
E
URA 620 CNRS, Structure et Mutagenèse Chromosomiques, Institut Curie, Paris, France.
Li
Z L
ZL
Dutrillaux
B
B
Apiou
F
F
Paulin
D
D
D001710:Biotin; D002874:Chromosome Mapping / Q000379:methods*; D002889:Chromosomes, Human, Pair 2 / Q000648:ultrastructure*; D015342:DNA Probes; D003893:Desmin / Q000235:genetics*; D005455:Fluorescent Antibody Technique; D006801:Humans; D009693:Nucleic Acid Hybridization
D015342:DNA Probes; D003893:Desmin; D001710:Biotin