Proteinic prorenin-releasing-stimulator (PRS) in the rat submandibular gland.
The release of prorenin as well as renin from rat renal slices was confirmed by a rat prorenin-prosegment ELISA system and an assay system for determining the renin activity. A significant increase of the prorenin release was found by adding rat submandibular gland extract to the slice medium, indicating the existence of a prorenin-releasing stimulator (PRS) in the extract. The pI and molecular mass of PRS were 8.5-8.7 and 28-30 kDa, respectively. The PRS was completely inactivated by boiling or a proteinase treatment.
Suzuki
F
F
Department of Biotechnology, Faculty of Agriculture, Gifu University, Japan.
Miyazaki
S
S
Uozumi
M
M
Okamoto
N
N
Yamashita
S
S
Murakami
K
K
Nakamura
Y
Y
D000818:Animals; D002850:Chromatography, Gel; D003470:Culture Media; D010450:Endopeptidases; D004792:Enzyme Precursors / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D012083:Renin / Q000378:metabolism*; D013363:Submandibular Gland / Q000378:metabolism*; D014020:Tissue Extracts / Q000494:pharmacology*; D014357:Trypsin
D003470:Culture Media; D004792:Enzyme Precursors; D014020:Tissue Extracts; D010450:Endopeptidases; D014357:Trypsin; D012083:Renin