Lineage-specific requirement of c-abl function in normal hematopoiesis.
Structural abnormalities of the c-abl proto-oncogene are found in hematopoietic cells of more than 90 percent of individuals with chronic myelogenous leukemia. Therefore c-abl may be important in normal as well as malignant hematopoiesis. Normal human hematopoietic progenitor cells were exposed to three different c-abl sense or antisense oligodeoxynucleotides, and the effects on myeloid and erythroid colony formation were examined. The c-abl antisense oligodeoxynucleotides inhibited myeloid, but not erythroid, colony formation. The c-abl sense oligodeoxynucleotides and bcr sense and antisense oligodeoxynucleotides were not inhibitory in this assay. These data show that c-abl is critical in normal myelopoiesis and may explain the relatively selective expansion of leukocytes in patients with chronic myelogenous leukemia.
Caracciolo
D
D
Istituto di Medicina ed Oncologia Sperimentale, Sezione di Ematologia, Torino, Italy.
Valtieri
M
M
Venturelli
D
D
Peschle
C
C
Gewirtz
A M
AM
Calabretta
B
B
D001773:Blood Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D001853:Bone Marrow; D002454:Cell Differentiation / Q000187:drug effects; D003114:Colony-Forming Units Assay; D004920:Erythropoiesis / Q000187:drug effects; D006410:Hematopoiesis / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D006801:Humans; D009841:Oligonucleotides / Q000494:pharmacology; D016376:Oligonucleotides, Antisense; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis; D011519:Proto-Oncogenes
C000715470:MAS1 protein, human; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins