title: Escherichia coli carbamoyl-phosphate synthetase: domains of glutaminase and synthetase subunit interaction.
abstract: Three catalytic domains of the Escherichia coli carbamoyl-phosphate synthetase (EC 6.3.5.5) have been identified in previous studies. These include the glutamine amide-N transfer domain in the carboxyl-terminal half of the glutaminase component and at least two adenine nucleotide binding sites in the synthetase component. To delineate the domains involved in subunit interactions, we have examined the effects of deletions and point mutations in the glutaminase and synthetase subunits on formation of the alpha beta holoenzyme. Deletion of the amino-terminal third of the glutaminase subunit abolishes interactions with the synthetase subunit, suggesting that this domain functions to stabilize the complex. Two subunit binding domains have been identified in the synthetase subunit. They are homologous to one another and are located in the amino-terminal and central regions of the synthetase component. These domains are adjacent to regions of the synthetase previously proposed to be involved in ATP binding and, possibly, activation of CO2. The new data enlarge the definition of the structural and functional domains in the two interdependent components of carbamoyl-phosphate synthetase.
authors.affiliation: Department of Molecular Genetics, Public Health Research Institute, New York, NY 10016.
authors.forename: F
authors.initials: F
authors.lastname: Guillou
authors.forename: S D
authors.initials: SD
authors.lastname: Rubino
authors.forename: R S
authors.initials: RS
authors.lastname: Markovitz
authors.forename: D M
authors.initials: DM
authors.lastname: Kinney
authors.forename: C J
authors.initials: CJ
authors.lastname: Lusty
mesh_terms: D002223:Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) / Q000235:genetics / Q000378:metabolism*; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000502:physiology; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005798:Genes, Bacterial; D005838:Genotype; D005972:Glutaminase / Q000378:metabolism*; D046911:Macromolecular Substances; D009154:Mutation; D010957:Plasmids; D011485:Protein Binding
chemical_list: D046911:Macromolecular Substances; D005972:Glutaminase; D002223:Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)