[Effect of lipopolysaccharide toxin on lipid and protein composition of human serum low density lipoproteins].
Complexes of lipopolysaccharide (LPS) B of Salmonella typhimurium with human low density lipoproteins (LDL) formed during in vitro coincubation via spontaneous incorporation of LPS (complex LDL-LPS) or through the incorporation stimulated by the serum protein fraction (LPS/LDL complex) were studied. The LPS/LDL complex was shown to maximally bind 0.24 mg of LPS per 1 mg of LDL protein, whereas the LDL-LPS complex contained only 0.07 mg of LPS per 1 mg of LDL protein. The observed incorporation of LPS into LDL particles was not possibly associated with a transfer of lipids or proteins from high density lipoproteins to LDL. The insertion of LPS was probably accompanied by the expulsion of a small portion of phosphatidylcholine molecules from the outer monolayer of LDL into the aqueous medium and by an increase in the phosphatidylethanolamine concentration in LDL. Simultaneously, the level of esterified cholesterol in the LPS/LDL complex decreased, and the concentrations of free cholesterol and triacylglycerols showed a rise. The level of free fatty acids in the LPS/LDL complex increased more than twofold compared with intact LDL. The enhancement of LPS incorporation did not result in the insertion of any serum proteins into LDL, in which apoB-100 remained the major apolipoprotein (ca. 90%); apoB-100 fragments made up to 5-7%, whereas apoE and apoC contained altogether ca. 3-5%. It is suggested that the LPS/LDL complex obtained can bind to three types of cell receptors, i.e., apoB/E receptors, LPS receptors and scavenger receptors of macrophages (monocytes); the increased level of free fatty acids in the LPS/LDL complex may accelerate its subsequent catabolism.
Viktorov
A V
AV
Gladkaia
E M
EM
Iurkiv
V A
VA
D001798:Blood Proteins / Q000378:metabolism*; D006801:Humans; D008055:Lipids / Q000097:blood*; D008070:Lipopolysaccharides / Q000633:toxicity*; D008077:Lipoproteins, LDL / Q000097:blood*; D008934:Mitogens / Q000633:toxicity*; D012486:Salmonella typhimurium / Q000032:analysis
D001798:Blood Proteins; D008055:Lipids; D008070:Lipopolysaccharides; D008077:Lipoproteins, LDL; D008934:Mitogens; C036757:lipopolysaccharide B
Vliianie lipopolisakharidnogo toksina na lipidnyĭ i belkovyĭ sostav syvorotochnykh lipoproteinov nizkoĭ plotnosti cheloveka.