title: Porcine circovirus antigens in PK-15 cell line (ATCC CCL-33) and evidence of antibodies to circovirus in Canadian pigs.
abstract: Permanent infection of a PK-15 (ATCC CCL-33) cell line by the porcine circovirus was demonstrated. This virus appears to be common in the Canadian swine population as indicated by the presence of antibodies detected by indirect immunofluorescence or immunoperoxidase tests in the serum of culled sows and sows in commercial herds. One source of the PK-15 cell line was found to be free of circovirus antigen.
authors.affiliation: Agriculture Canada, Animal Diseases Research Institute, NEPEAN, Ontario.
authors.forename: G C
authors.initials: GC
authors.lastname: Dulac
authors.forename: A
authors.initials: A
authors.lastname: Afshar
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis*; D002170:Canada / Q000453:epidemiology; D002460:Cell Line; D004267:DNA Viruses / Q000276:immunology*; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D007106:Immune Sera / Q000276:immunology; D007124:Immunoenzyme Techniques / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology*; D014777:Virus Diseases / Q000453:epidemiology / Q000662:veterinary*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D007106:Immune Sera