title: Fractionation, characterization and diagnostic potential of filarial antigens isolated from hydrocoele fluid in bancroftian filariasis.
abstract: Filarial antigen was isolated from hydrocoele fluid and fractionated on Ultrogel AcA 44. Six protein fractions (HFA C1 to HFA C6) were chromatographically separated from the column. Of the 4 antigenic fractions (HFA C1, HFA C2, HFA C3 and HFA C5), HFA C3 was a major reactive fraction with filarial serum immunoglobulin G. Analysis of HFA C3 by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) yielded 18 bands of Mr 18,000-160,000. Two fractions, HFA C3-7 and HFA C3-9 of Mr 48,000-55,000 and 32,000-39,000 obtained by gel elution, were antigenic in an enzyme-linked immunosorbent assay (ELISA). SDS-PAGE immunoblotting confirmed the ELISA by identifying 3 immunoreactive bands of Mr 51,000, 38,000 and 35,000. The diagnostic potential of HFA C3-7 and HFA C3-9 was compared in serodiagnosis of active infections and diseased states. HFA C3-9 showed greater sensitivity in detection of filarial specific IgM antibody in cases with microfilaraemia. Physicochemical characterization indicated the protein nature of HFA C3-9.
authors.affiliation: Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram, India.
authors.forename: P
authors.initials: P
authors.lastname: Ramaprasad
authors.forename: B C
authors.initials: BC
authors.lastname: Harinath
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000302:isolation & purification*; D004605:Elephantiasis, Filarial / Q000175:diagnosis / Q000276:immunology* / Q000469:parasitology; D005368:Filariasis / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D012698:Serologic Tests; D006848:Testicular Hydrocele / Q000276:immunology; D014957:Wuchereria / Q000276:immunology*; D014958:Wuchereria bancrofti / Q000276:immunology* / Q000469:parasitology
chemical_list: D000947:Antigens, Helminth; D007074:Immunoglobulin G