Evaluation of time-resolved fluoroimmunoassay with Eu-labelled protein-A for serum progesterone.
Progesterone has been assayed in several serum samples by a time-resolved fluoroimmunoassay (TR-FIA). The solid phase was 11 alpha-hydroxyprogesterone hemisuccinate bound covalently to ovalbumin and adsorbed on wells of polystyrene. The assay was based on competitive reaction of solid phase-bound hormone and samples with specific antibody labelled in situ with protein-A prelabelled with europium. The bound Eu was dissociated from the solid phase by an enhancement solution and measured by time-resolved fluorometry. The coefficient of correlation between TR-FIA and RIA was 0.97.
Ius
A
A
Istituto di Chimica degli Ormoni, C.N.R., Milano, Italy.
Ferrara
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L
Meroni
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G
Bacigalupo
M A
MA
D005063:Europium; D005455:Fluorescent Antibody Technique; D006801:Humans; D007202:Indicators and Reagents; D011374:Progesterone / Q000097:blood*; D013205:Staphylococcal Protein A; D013997:Time Factors
D007202:Indicators and Reagents; D013205:Staphylococcal Protein A; D005063:Europium; D011374:Progesterone