[Stability to the extreme influences and significance of internal pH of Saccharomyces cerevisiae depending on the density of cellular suspensions].
The resistance of a Saccharomyces cerevisiae population against gamma-irradiation (400 krd), heating (60 degrees C), repeated freezing--thawing and rehydration of dehydrated cells was shown to depend on the density of a cell suspension. The percentage of damaged cells (stained with primulin) was found to increase in a suspension with decreasing cell concentrations. The effect was also exerted in an oxygen-free medium. The percentage of undamaged cells increased in populations with a lower cell concentration when a more viscous medium (0.2% agar) was used. The intracellular pH dropped down in a denser intact yeast suspension and rose with an increase in the concentration of dehydrated-rehydrated cells.
Zikmanis
P B
PB
Auzinia
L P
LP
Kruche
R V
RV
Margevicha
M V
MV
Auzan
S I
SI
Beker
M E
ME
D005615:Freezing; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008826:Microbial Sensitivity Tests; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000254:growth & development / Q000528:radiation effects
Ustoĭchivost' k ékstremal'nym vozdeĭstviiam i znachenie vnutrennego pH Saccharomyces cerevisiae v zavisimost ot plotnosti kletochnykh suspenziĭ.