title: Use of a novel air separation system in a fed-batch fermentative culture of Escherichia coli.
abstract: A novel air separation system based on permeable membrane gas separation technology was used to cultivate Escherichia coli. The system fulfilled the dissolved oxygen requirements of a culture of E. coli grown on a glucose synthetic medium at a high and constant growth rate of 0.55 h-1. A biomass yield of 45 g (dry weight) per liter was achieved, and no by-product inhibition by acetate or CO2 was observed.
authors.affiliation: Biotechnology Unit, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892.
authors.forename: R
authors.initials: R
authors.lastname: Fass
authors.forename: T R
authors.initials: TR
authors.lastname: Clem
authors.forename: J
authors.initials: J
authors.lastname: Shiloach
mesh_terms: D003470:Culture Media; D004926:Escherichia coli / Q000254:growth & development* / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D010100:Oxygen / Q000378:metabolism*
chemical_list: D003470:Culture Media; D005947:Glucose; D010100:Oxygen