[Cellular lectin binding sites in the human retinal pigment epithelium and photoreceptors--method of lectin histochemistry and results with peroxidase marked lectins in paraffin sections].
Lectins are sugar-binding proteins or glycoproteins which bind to specifically complementary sugar residues found in cell membranes and the cytoplasm. Lectin-binding sites are determined by histochemical methods. Cellular lectin-binding sites change with morphological and functional alterations of the cell, and the determination of lectin receptors can be used to identify specific cell types in histological sections. Lectin-binding sites of the human retinal pigment epithelium (RPE) and photoreceptor cells under normal and pathological conditions have not been examined. Lectin-binding sites of normal retinal pigment epithelium and photo-receptor cells obtained from enucleated human bulbi were identified by a modified PAP method. Eight lectins differing in their sugar affinity (Con A, WGA, PNA, RCA I, SBA, UEA, DBA, LPA) were used to investigate a wide range of glycoconjugate specificity. Normal RPE have receptors for the lectins Con A, WGA, PNA and RCA I, mostly localized in the apical portions of the cells. Binding sites for these lectins are also found on photoreceptor cells, but with different binding characteristics. Photoreceptor cells bind additional lectins (UEA, SBA, DBA), which are not found on the RPE.
Bopp
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S
el-Hifnawi
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E
Laqua
H
H
D006801:Humans; D007124:Immunoenzyme Techniques; D010786:Photoreceptor Cells / Q000033:anatomy & histology*; D010857:Pigment Epithelium of Eye / Q000033:anatomy & histology*; D011975:Receptors, Mitogen / Q000032:analysis*; D012016:Reference Values
D011975:Receptors, Mitogen
Zelluläre Lektinbindungsstellen am menschlichen retinalen Pigmentepithel und an Photorezeptoren--Methodik der Lektinhistochemie und Ergebnisse mit Peroxidase-markierten Lektinen an Paraffinschnitten.