[Complementary addressed alkylation of 16s rRNA from Escherichia coli by 2',3'-0-[4-N-(2-chloroethyl)-N-methylamino]benzylidene derivatives of oligodeoxyribonucleotides. III. Segments of 16s rRNA interacting with the benzylidene derivative of d(pACCTTGTT)rA].
16S rRNA chain was cleaved by RNase H within covalent adduct with a 2',3'-0-[4-N-(2-chloroethyl)-N-methylamino]benzylidene derivative of d(pACCTTGTT)rA. It was shown that no less than 50% of cleavage occurs at the 1498-1506 site. The selectivity of alkylation and correspondingly the cleavage by RNase H, at this site practically does not increase when RNA was alkylated to a low extent, and also when a small excess of the reagent in respect to rRNA was used.
Zenkova
M A
MA
Karpova
G G
GG
Levina
A S
AS
D000478:Alkylation; D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009588:Nitrogen Mustard Compounds / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000378:metabolism*
D009588:Nitrogen Mustard Compounds; D009838:Oligodeoxyribonucleotides; D012335:RNA, Ribosomal; D012336:RNA, Ribosomal, 16S
Komplementarno adresovannoe alkilirovanie 16S rRNK Escherichia coli 2',3'-0-[4-N-(2-khlorétil)-N-metilamino]benzilidenovymi proizvodnymi oligodezoksiribonukleotidov. III. uchastki 16S rRNA, vzaimodeĭstvuiushchie s benzilidenovym proizvodnym d(pACCTTGTT)rA.