Two different anti-erythroid monoclonal antibodies in immunodiagnosis of human leukemias: a comparative study.
To date, only anti-glycophorin-A monoclonal antibodies (MAbs) have been widely used as anti-erythroid probes in the diagnosis of leukemias. We have examined blood, bone-marrow and lymph-node samples from 474 patients, adults and children, with different hemopoietic malignancies, using a panel of MAbs including 2 anti-erythroid MAbs directed to glycophorin-A and an antigen of erythroblasts, Ag-Eb. MAb HAE9 directed against a human epitope of Ag-Eb has earlier been shown to be highly specific for immature erythroid cells. Of all the patients, 2.7% demonstrated glycophorin-A expression on blast cells, while anti-Ag-Eb MAb HAE9 reacted positively with cells from 6.0% of patients. Samples from 31 of 474 (6.5%) patients expressed one or both erythroid markers. Our results indicate that MAb HAE9 may be useful, in combination with anti-glycophorin-A MAbs, as an anti-erythroid probe for immunophenotyping human leukemias.
Tupitsyn
N N
NN
Cancer Research Center, Moscow, USSR.
Mechetner
E B
EB
Baryschnikov AJu
Drozdova
T S
TS
Frenkel
M A
MA
Ievleva
E S
ES
Kiselev
A V
AV
Perilov
A A
AA
Peterson
I S
IS
Probatova
N A
NA
D000328:Adult; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000951:Antigens, Neoplasm / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D014408:Biomarkers, Tumor / Q000032:analysis; D001853:Bone Marrow / Q000276:immunology; D002648:Child; D015672:Erythroid Precursor Cells / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007159:Immunologic Tests / Q000379:methods; D007938:Leukemia / Q000175:diagnosis*; D008198:Lymph Nodes / Q000276:immunology; D010641:Phenotype
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D014408:Biomarkers, Tumor