[The study of binding of the virG gene product with the virD gene promotor region of the Agrobacterium tumefaciens Ti-plasmid C58].
The 1.5 kb EcoRI--HindIII fragment of the pTiC58 containing the virD regulatory sequence demonstrates a constitutive promoter activity in E. coli background and an inducible one in agrobacterium. The virG gene was cloned in pTZ19R plasmid. To reveal the virG product--virD regulatory sequence interaction a few protein fractions of E. coli harbouring the obtained recombinant plasmid pTZ19G lysate were used. PAGE-retardation assay revealed the specific binding between the 1.5 kb DNA fragment containing 5'-end of virD and a separate protein fraction of the bacterial lysate.
Li
K G
KG
Kobets
N S
NS
Andrianov
V M
VM
Piruzian
D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism*; D003001:Cloning, Molecular; D004586:Electrophoresis; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D012231:Rhizobium / Q000235:genetics*
D001426:Bacterial Proteins
Izuchenie sviazyvaniia produkta gena virG s promotornoĭ oblast'iu gena virD Ti-plazmidy Agrobacterium tumefaciens C58.