Estrogen and progesterone receptor assays, by the EIA method (Abbott), of low-speed supernates of breast-cancer homogenates.
Estrogen receptor (ER) and progesterone receptor (PR) assays were performed by solid-phase enzyme immunoassays (Abbott) on low-speed (2600 x g) and high-speed (100,000 x g) supernates of breast-cancer homogenates. The results were similar, indicating that ultracentrifugation is not essential for ER and PR assays with the Abbott kit.
Hähnel
R
R
Department of Obstetrics & Gynaecology, University of Western Australia, King Edward Memorial Hospital for Women, Subiaco.
Avenell
M H
MH
Hähnel
E
E
D001943:Breast Neoplasms / Q000032:analysis*; D002498:Centrifugation; D003600:Cytosol / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D011786:Quality Control; D011933:Reagent Kits, Diagnostic; D011960:Receptors, Estrogen / Q000032:analysis*; D011980:Receptors, Progesterone / Q000032:analysis*
D011933:Reagent Kits, Diagnostic; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone