Development of Vero cell miniculture assay for detection of heat-labile enterotoxin of Escherichia coli and its significance.
In the present study the Vero cell miniculture assay was developed for the detection of heat-labile enterotoxin of E. coli. The test was found to be reliable and efficient and was comparable with other tissue culture assays. The new cell lines BHK21, MDBK, LM and CK included in the present study did not respond positively to the E. coli toxins.
Choudhry
M A
MA
Division of Microbiology, Central Drug Research Institute, Lucknow, India.
Yadava
J N
JN
Negi
B S
BS
Yadava
M P
MP
D000818:Animals; D001427:Bacterial Toxins / Q000032:analysis*; D002460:Cell Line; D004768:Enterotoxins / Q000032:analysis*; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D006801:Humans; D014709:Vero Cells
D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C027538:heat-labile enterotoxin, E coli