title: [Conformational stability and immunochemical properties of yersinin--a basic protein of the outer membrane of the Pseudotuberculosis microbe].
abstract: Using spectroscopic methods (circular dichroism and intrinsic protein fluorescence) and immunoenzyme assay, changes in the spatial and antigenic structure of yersinin, porin from outer membrane of Yersinia pseudotuberculosis, were studied in solutions of ionic and non-ionic detergents at various temperatures and low pH values. Yersinin was shown to retain its secondary structure under various denaturation conditions, the content of regular structural patterns depending on specific action of the denaturation agent. Process of yersinin denaturation similarly to other membrane proteins appears to occur via two structural transitions: dissociation of oligomers and denaturation of monomers. At the first stage changes of quaternary structure accompanied by the loss of so called conformational determinants were observed. Temperature-dependent changes of monomers' tertiary structure affect antigenic activity of yersinin in a smaller degree.
authors.forename: O D
authors.initials: OD
authors.lastname: Novikova
authors.forename: G M
authors.initials: GM
authors.lastname: Frolova
authors.forename: T I
authors.initials: TI
authors.lastname: Vakorina
authors.forename: Z A
authors.initials: ZA
authors.lastname: Tarankova
authors.forename: V P
authors.initials: VP
authors.lastname: Glazunov
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000032:analysis; D002942:Circular Dichroism; D003902:Detergents; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D007124:Immunoenzyme Techniques; D046911:Macromolecular Substances; D011487:Protein Conformation; D011489:Protein Denaturation; D013329:Structure-Activity Relationship; D015011:Yersinia pseudotuberculosis / Q000032:analysis*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D003902:Detergents; D046911:Macromolecular Substances
vernacular_title: Konformatsionnaia stabil'nost' i immunokhimicheskie svoĭstva iersinina--osnovnogo belka vneshneĭ membrany psevotuberkuleznogo mikroba.