[Comparison of the specificity of polyclonal antibodies to the tick-borne encephalitis virus in immunoenzyme analysis and immunoblotting].
The present study deals with the optimization of the conditions of immunoblotting (IB) and the enzyme-linked immunosorbent assay (ELISA) with the use of mouse polyclonal immunoglobulins to tick-borne encephalitis virus. In contrast to ELISA, the results of IB depended, to a great extent, on the composition and pH of blocking buffer mixtures. In some cases IB permitted the detection of a heretofore unknown virus-specific polypeptide with a molecular weight of 58-60 KD. The results of the study lead to the conclusion on the impossibility of the direct transfer of data concerning the specificity of individual preparations of immunoglobulins in ELISA or IB due to differences in information.
Terletskaia
E N
EN
Timofeev
A V
AV
Karavanov
A S
AS
Mal'dov
D G
DG
El'bert
D B
DB
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000918:Antibody Specificity; D004669:Encephalitis Viruses, Tick-Borne / Q000276:immunology*; D005069:Evaluation Studies as Topic; D007114:Immunization; D015151:Immunoblotting / Q000379:methods; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight
D000914:Antibodies, Viral
Sravnenie spetsifichnosti poliklonal'nykh antitel k virusu kleshchevogo éntsefalita v immunofermentnom analize i immunoblottinge.