title: [Immunological humoral response in mice experimentally infected with Echinococcus granulosus, preliminary result of kinetic observation].
abstract: 5 serological methods included latex agglutination test (LA), enzyme-linked immunoelectrotransfer blot (EITB), enzyme-linked immunosorbent assay (ELISA), dot-enzyme-linked immunosorbent assay (Dot-ELISA) and immunofluorescence antibody test (IFA) were used to trace the kinetic changes of the humoral response in NIH mice experimentally infected with protoscolex of Echinococcus granulosus. Results showed that Dot-ELISA and IFA were able to detect antibodies 2 wk after infection while ELISA, EITB and LA gave positive reactions 4 wk, 2 mos and 3 mos after infection respectively. Therefore Dot-ELISA and IFA were considered to be the most sensitive ones among the 5 methods used. All infected mice gave positive reactions at 8 and 12 mos but none of the normal control mice showed any reaction throughout the period of observation (Fig. 1).
authors.forename: H C
authors.initials: HC
authors.lastname: Xue
authors.forename: L S
authors.initials: LS
authors.lastname: Qiu
authors.forename: Y H
authors.initials: YH
authors.lastname: Zhang
authors.forename: C W
authors.initials: CW
authors.lastname: Zhang
authors.forename: Z X
authors.initials: ZX
authors.lastname: Zhu
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Yang
authors.forename: J A
authors.initials: JA
authors.lastname: Lu
authors.forename: H
authors.initials: H
authors.lastname: Li
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis*; D004443:Echinococcosis / Q000276:immunology*; D004446:Echinococcus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005455:Fluorescent Antibody Technique; D007841:Latex Fixation Tests; D051379:Mice
chemical_list: D000909:Antibodies, Helminth