title: The acylated precursor form of the colicin A lysis protein is a natural substrate of the DegP protease.
abstract: The acylated precursor form of the colicin A lysis protein (pCalm) is specifically cleaved by the DegP protease into two acylated fragments of 6 and 4.5 kilodaltons (kDa). This cleavage was observed after globomycin treatment, which inhibits the processing of pCalm into mature colicin A lysis protein (Cal) and the signal peptide. The cleavage took place in lpp, pldA, and wild-type strans carrying plasmids which express the lysis protein following SOS induction and also in cells containing a plasmid which expresses it under the control of the tac promoter. Furthermore, the DegP protease was responsible for the production of two acylated Cal fragments of 3 and 2.5 kDa in cells carrying plasmids which overproduce the Cal protein, without treatment with globomycin. DegP could also cleave the acylated precursor form of a mutant Cal protein containing a substitution in he amino-terminal portion of the protein, but not that of a mutant Cal containing a frameshift mutation in its carboxyl-terminal end. The functions of Cal in causing protein release, quasi-lysis, and lethality were increased in degP41 cells, suggesting that mature Cal was produced in higher amounts in the mutant than in the wild type. These effects were limited in cells deficient in phospholipase A. Interactions between the DegP protease and phospholipase A were suggested by the characteristics of degP pldA double mutants.
authors.affiliation: Centre de Biochimie Biologie Moléculaire du Centre National de la Recherche Scientifique, Marseille, France.
authors.forename: D
authors.initials: D
authors.lastname: Cavard
authors.forename: C
authors.initials: C
authors.lastname: Lazdunski
authors.forename: S P
authors.initials: SP
authors.lastname: Howard
mesh_terms: D000215:Acylation; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D010450:Endopeptidases / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D006360:Heat-Shock Proteins; D007700:Kinetics; D009154:Mutation; D033903:Periplasmic Proteins; D010957:Plasmids; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D012697:Serine Endopeptidases; D013045:Species Specificity; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; D033903:Periplasmic Proteins; D011498:Protein Precursors; C061680:colicin A lysis protein precursor, Bacteria; D010450:Endopeptidases; C061682:DegP protease; D012697:Serine Endopeptidases