Identification of Escherichia coli that produces heat-stable enterotoxin STA by a commercially available enzyme-linked immunoassay and comparison of the assay with infant mouse and DNA probe tests.
By using the infant mouse test and a commercially available competitive enzyme-linked immunosorbent assay (ELISA), 100 strains of Escherichia coli carrying STA1 or STA2 genes were shown to produce the heat-stable enterotoxin STA. An additional 100 strains were negative in both tests. The ELISA was easy to perform, and results were available within 24 h. Testing strains with an enzyme-linked DNA probe kit that incorporated both STA1- and STA2-specific oligonucleotides showed that the 100 strains positive in the mouse test and ELISA also hybridized with the mixed probe. Two strains carrying STA1 genes but negative in the mouse test and ELISA also hybridized with the mixed probe.
Scotland
S M
SM
Division of Enteric Pathogens, Central Public Health Laboratory, London, England.
Willshaw
G A
GA
Said
B
B
Smith
H R
HR
Rowe
B
B
D000818:Animals; D000831:Animals, Newborn; D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000235:genetics; D001681:Biological Assay; D015342:DNA Probes; D004768:Enterotoxins / Q000096:biosynthesis* / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D029968:Escherichia coli Proteins; D051379:Mice; D009693:Nucleic Acid Hybridization; D011933:Reagent Kits, Diagnostic
D001427:Bacterial Toxins; D015342:DNA Probes; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D011933:Reagent Kits, Diagnostic; C034151:heat stable toxin (E coli)