Comparative analysis of alternating purine-pyrimidine sites in yeast chromosomes.
It is known that under some circumstances DNA sequences made up of purine and pyrimidines in repeated alternation can undergo a transition from the right-handed to a left handed helical configuration which has been called Z-DNA. We have analyzed yeast chromosomal DNAs for the presence of alternating purine-pyrimidine stretches using a new strategy. Our strategy takes advantage of pulsed field gel electrophoresis (PFGE) to separate yeast chromosomal DNAs. They are subsequently analyzed by molecular hybridization with radioactive (dC-dA)n.(dG-dT)n or (dG-dC)n.(dC-dG)n probes, autoradiography and densitometric scanning. Preliminary results obtained by the application of the new method to Saccharomyces cerevisiae are described.
Rollo
F
F
Dipartimento di Biologia Cellulare, Camerino.
Vaughan Martini
A
A
Martini
A
A
Pacilli
A
A
Ferracuti
T
T
Amici
A
A
D001345:Autoradiography; D015139:Blotting, Southern; D015342:DNA Probes; D004271:DNA, Fungal / Q000235:genetics*; D003720:Densitometry; D004587:Electrophoresis, Agar Gel; D009693:Nucleic Acid Hybridization; D011685:Purine Nucleotides; D011742:Pyrimidine Nucleotides; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D015342:DNA Probes; D004271:DNA, Fungal; D011685:Purine Nucleotides; D011742:Pyrimidine Nucleotides