The neuronal response to injury as visualized by immunostaining of class III beta-tubulin in the rat.
The neuronal response to trauma of the brain and spinal cord was examined by staining sections of injured central nervous system (CNS) with a monoclonal antibody (TuJ1) that recognizes class III beta-tubulin exclusively. Because class III beta-tubulin is expressed by neurons and not by glia, this monoclonal antibody stains neuronal cell bodies, dendrites, axons and axonal terminations darkly with a pale staining background. Thus, the TuJ1 antibody is extremely useful, revealing the fine details of axons and their terminations, as well as significant injury-related alterations in the composition of the somatic cytoskeleton.
Geisert
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Department of Cell Biology and Anatomy, Neurobiology Research Center, University of Alabama, Birmingham 35294.
Frankfurter
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A
D000818:Animals; D000911:Antibodies, Monoclonal; D001369:Axons / Q000032:analysis* / Q000648:ultrastructure; D001921:Brain / Q000648:ultrastructure*; D001923:Brain Chemistry; D007124:Immunoenzyme Techniques; D009410:Nerve Degeneration; D009474:Neurons / Q000032:analysis* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D014404:Tubulin / Q000032:analysis*
D000911:Antibodies, Monoclonal; D014404:Tubulin