Convenient preparative synthesis of [14C]trehalose from [14C]glucose by intact Escherichia coli cells.
At high osmolarity, Escherichia coli synthesizes trehalose intracellularly, irrespective of the nature of the carbon source. Synthesis proceeds via the transfer of UDP-glucose to glucose 6-phosphate, yielding trehalose 6-phosphate, followed by its dephosphorylation to trehalose (H.M. Giaeyer, B.O. Styrvold, I. Kaasen, and A.R. Strøm, J. Bacteriol. 170:2841-2849, 1988). This reaction was exploited to preparatively synthesize [14C]trehalose from exogenous [14C]glucose by using intact bacteria of a mutant (DF214) that could not metabolize glucose. The total yield of radiochemically pure trehalose from glucose was routinely more than 50%.
Brand
B
B
Department of Biology, University of Konstanz, Federal Republic of Germany.
Boos
W
W
D001692:Biological Transport; D002250:Carbon Radioisotopes; D002855:Chromatography, Thin Layer; D004187:Disaccharides; D004926:Escherichia coli / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D007700:Kinetics; D014199:Trehalose / Q000096:biosynthesis* / Q000378:metabolism
D002250:Carbon Radioisotopes; D004187:Disaccharides; D014199:Trehalose; D005947:Glucose