Extracorporeal hybridization of proximal renal tubular cells and an artificial membrane for the purpose of beta 2 microglobulin removal.
The present study was designed to develop a bioartificial kidney hybridized with LLC-PK1 cells for the purpose of beta 2microglobulin (B2M) removal. Cells were prepared in polystyrene culture wells; the bottom of each was covered with collagen fiber and the cells grown as monolayer cultures. Each well was inoculated with 1 ml cell suspensions with 0.0, 1.0 X 10(6), 2.0 X 10(6), or 4.0 X 10(6) cells/well. B2M was added to the culture system at a concentration of 80 micrograms/dl. Results were as follows: 1) Concentration of B2M in the supernatant of 4 X 10(6) cultivated LLC-PK1 cells was decreased from 79.32 +/- 2.41 micrograms/dl to 67.02 +/- 3.00 micrograms/dl (n = 6, p less than 0.01) at 2 hr; 2) Concentration of B2M in the supernatant of 1 X 10(6), 2 X 10(6), and 4 X 10(6) cultivated LLC-PK1 cells was 75.06 +/- 2.31 micrograms/dl (n = 6, p less than 0.05); 67.27 +/- 11.41 micrograms/dl (n = 6, p less than 0.001); and 67.02 +/- 3.00 micrograms/dl (n = 6, p less than 0.001), respectively, vs. 79.32 +/- 2.41 micrograms/dl in controls at 2 hr; 3) There was a significant positive correlation between the efficacy of B2M removal and cell number; and 4) These results suggested that it is possible to develop an artificial kidney hybridized with proximal tubular cells that possesses a high affinity for B2M removal.
Sanaka
T
T
Kidney Center, Tokyo Women's Medical College, Japan.
Agishi
T
T
Hayashi
T
T
Hayasaka
Y
Y
Yasuo
M
M
Fukui
K
K
Ota
K
K
Sugino
N
N
D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D006822:Hybrid Cells / Q000166:cytology*; D007687:Kidney Tubules, Proximal / Q000166:cytology*; D007688:Kidneys, Artificial; D008567:Membranes, Artificial; D011188:Potassium / Q000378:metabolism; D012709:Serum Albumin / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D013552:Swine; D001613:beta 2-Microglobulin / Q000378:metabolism*
D008567:Membranes, Artificial; D012709:Serum Albumin; D001613:beta 2-Microglobulin; D012964:Sodium; D011188:Potassium