Automated analysis of 35-S-methionine labeled proteins by SDS-PAGE as a typing method in a suspected cluster of Serratia marcescens.
During a nine-month period in 1986, we observed five ornithine decarboxylase-negative isolates of Serratia marcescens from four different patients. All isolates were identical in more than 50 biochemical parameters. Four isolates from three hospitalized patients were essentially identical in their susceptibility patterns to 12 antimicrobial agents. Analysis of 35-S-methionine labeled whole cell proteins by SDS-PAGE with the AMBIS system (Automated Microbiology System Inc., San Diego, CA) suggested that only three of these isolates were identical while the fourth hospital strain was more closely related to the isolate of a patient with no contact to the hospital. These results were confirmed by bacteriocin- and serotyping. We conclude that analysis of these protein patterns--which does not require special reagents - was an adequate method for typing S. marcescens.
Altwegg
M
M
Department of Medical Microbiology, University of Zürich, Switzerland.
Lüthy-Hottenstein
J
J
Grehn
M
M
von Graevenitz
A
A
D001426:Bacterial Proteins / Q000032:analysis*; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D006801:Humans; D008297:Male; D012703:Serotyping; D012706:Serratia marcescens / Q000032:analysis / Q000145:classification* / Q000187:drug effects
D001426:Bacterial Proteins