title: Effect of recombinant human interleukin 2 (rIL-2) on normal peripheral blood B cells and B lymphoblastoid cell lines.
abstract: The role of interleukin 2 (IL-2) for growth and differentiation of normal and malignant B cells still remains controversial. We assessed normal peripheral blood B cells and cell lines derived from patients with B non-Hodgkin's lymphomas (NHLs) with respect to their responsiveness to recombinant human IL-2 (rIL-2). The NHL cell lines used in our experiments expressed the Tac antigen (CD25)--a compound of the IL-2 receptor (IL-2R)--in a percentage ranging from 28 to 57%. As measured in a [3H]thymidine uptake assay, the normal peripheral blood B cells demonstrated a dose-dependent proliferative response to rIL-2, whereas the NHL cells did not show any responsiveness to rIL-2. In a clonogenic culture assay we evaluated the colony formation of the NHL cells and found a decrease of 28 to 41% on average in the presence of rIL-2 (10-50 U/ml). This moderate inhibitory effect on the clonal growth of the NHL cells was not due to a differentiation inducing effect of rIL-2, as studied by measuring the Ig production under increasing doses of rIL-2 (1 to 100 U/ml). Thus, malignant NHL B cells may express the CD25 compound of the IL-2 receptor on their surface, demonstrating a different functional responsiveness to rIL-2 compared to normal peripheral blood B cells.
authors.affiliation: Department of Internal Medicine V, University of Heidelberg, F.R.G.
authors.forename: R
authors.initials: R
authors.lastname: Haas
authors.forename: S
authors.initials: S
authors.lastname: Hohaus
authors.forename: S
authors.initials: S
authors.lastname: Kiesel
authors.forename: E
authors.initials: E
authors.lastname: Ogniben
authors.forename: R
authors.initials: R
authors.lastname: Ehrhardt
authors.forename: W
authors.initials: W
authors.lastname: Hunstein
mesh_terms: D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000187:drug effects; D007376:Interleukin-2 / Q000494:pharmacology*; D008228:Lymphoma, Non-Hodgkin / Q000276:immunology*; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured
chemical_list: D007074:Immunoglobulin G; D007376:Interleukin-2; D011994:Recombinant Proteins