Characterization of the RepFVII replicon of the haemolytic plasmid pSU233: nucleotide sequence of an incFVII determinant.
We have isolated a replication region (designated RepFVII) from the IncFVII plasmid, pSU233. Hybridization experiments showed that RepFVII has high homology to the RepFIII replicon of the plasmid pSU316 and, therefore, that pSU233 is another member of the RepFIIA family. We have also located the incompatibility gene (incFVII) from RepFVII. The analysis of its sequence revealed the same organization described for several other replicons belonging to the same family.
López
J
J
Departamento de Biología Molecular, Facultad de Medicina, Polígono de Cazoña, Santander, Spain.
Rodríguez
J C
JC
Andrés
I
I
Ortiz
J M
JM
D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005144:F Factor; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D012093:Replicon; D012689:Sequence Homology, Nucleic Acid
D004269:DNA, Bacterial