Synthetic oligonucleotide probes complementary to the HLA-B variable region hybridize with Klebsiella pneumoniae.
Three oligonucleotide probes complementary to base sequences of the HLA-B variable region were used to probe clinical and foodborne isolates of Klebsiella spp. by DNA colony hybridization. One oligonucleotide (RR-3) corresponding to amino acid residues 66-74 of the HLA-B27.1 sequence and one corresponding to residues 66-74 of the HLA-B7 sequence (RR-5) hybridized with K. pneumoniae under conditions of high stringency. A genomic library was constructed using K. pneumoniae K43 chromosomal DNA, and a 1 kb PstI restriction fragment was found to contain the sequence associated with specific binding of the oligonucleotide probes. After purification and radiolabeling, the cloned fragment hybridized with 96.2% of the K. pneumoniae isolates by DNA colony hybridization. These results confirm the presence of sequence similarities between bacterial DNA and the MHC Class I variable region.
Williams
K M
KM
Division of Microbiology, Food and Drug Administration, Washington, DC 20204.
Hirofuji
T
T
Raybourne
R B
RB
Shook
D L
DL
Trucksess
M W
MW
Yu
D T
DT
D000942:Antigens, Bacterial / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D015235:HLA-B Antigens / Q000235:genetics*; D006801:Humans; D007711:Klebsiella pneumoniae / Q000235:genetics* / Q000276:immunology; D015345:Oligonucleotide Probes / Q000138:chemical synthesis / Q000235:genetics*; D010957:Plasmids; D012689:Sequence Homology, Nucleic Acid; D013167:Spondylitis, Ankylosing / Q000382:microbiology
D000942:Antigens, Bacterial; D004269:DNA, Bacterial; D015235:HLA-B Antigens; D015345:Oligonucleotide Probes