Comparison of a non-radioactive hybridization assay for detection of hepatitis B virus DNA with the radioactive method.
Biotin and 32P-labelled HBV probes were compared in 150 sera analysed with a spot hybridization test. In 144 cases (96%) results were concordant. The non-isotopic procedure was therefore not only simple and rapid to perform, but also sensitive and specific. Since it overcame the problems associated with 32P, it allowed more diagnostic applications of HBV-DNA determination in serum.
Quibriac
M
M
Laboratoire de Virologie, Centre Hospitalier Régional et Universitaire, Caen, France.
Petitjean
J
J
Thiers
V
V
Tiollais
P
P
Brechot
C
C
Freymuth
F
F
D001710:Biotin; D015342:DNA Probes; D004279:DNA, Viral / Q000097:blood*; D006515:Hepatitis B virus / Q000302:isolation & purification*; D006801:Humans; D015336:Molecular Probe Techniques; D009693:Nucleic Acid Hybridization; D010761:Phosphorus Radioisotopes; D011865:Radioisotope Dilution Technique
D015342:DNA Probes; D004279:DNA, Viral; D010761:Phosphorus Radioisotopes; D001710:Biotin