A human gallbladder adenocarcinoma cell line.
A cell strain (FU-GBC-1) was established from cancerous ascites of a 68-year-old male patient with well-differentiated adenocarcinoma of the gallbladder. By light and electron microscopy, the cultured cells showed the morphologic features of adenocarcinoma characterized by gland-like structures, intracellular microcystic spaces, and mucous production. Immunoperoxidase stains showed that FU-GBC-1 cells expressed several epithelial tumor antigens including CA 19-9, carcinoembryonic antigen (CEA), and epithelial membrane antigen (EMA). The cell strain has been in continuous culture up to passage 44 for 1 1/2 years, with the population doubling time of 120 hours. The cytogenetic analysis by a G-band technique showed a constant loss of chromosome Y in FU-GBC-1 cells. The modal chromosome number at passage 12 was 82 with a range of 77 to 85. Flow cytometry with an ethidium bromide technique additionally confirmed aneuploid DNA content (4C) in the cultured cells at passage 12 and 35. Inoculation of FU-GBC-1 cells into the dermis of BALB/c nude mice produced transplantable adenocarcinoma identical to the original tumor. Because no continuous cell lines of the well-differentiated type of gallbladder adenocarcinoma have been reported in the literature currently, the newly established cell strain we report may yield a useful system for studying the morphologic and biologic characteristics of gallbladder adenocarcinoma.
Johzaki
H
H
Department of Pathology, Fukuoka University School of Medicine, Japan.
Iwasaki
H
H
Nishida
T
T
Isayama
T
T
Kikuchi
M
M
D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology* / Q000648:ultrastructure; D000368:Aged; D000782:Aneuploidy; D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis; D002272:Carcinoembryonic Antigen / Q000032:analysis; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D004273:DNA, Neoplasm / Q000032:analysis; D005706:Gallbladder Neoplasms / Q000235:genetics / Q000473:pathology* / Q000648:ultrastructure; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008562:Membrane Glycoproteins; D051379:Mice; D008819:Mice, Nude; D018396:Mucin-1; D009368:Neoplasm Transplantation; D014407:Tumor Cells, Cultured; D014998:Y Chromosome
D000951:Antigens, Neoplasm; D002272:Carcinoembryonic Antigen; D004273:DNA, Neoplasm; D008562:Membrane Glycoproteins; D018396:Mucin-1