title: [Biosynthesis and secretion of bovine growth hormone in Escherichia coli under the control of the secretory vector containing a promoter and signal region of the alkaline phosphatase gene].
abstract: A recombinant plasmid carrying a bovine growth hormone gene fused with the regulatory and signal regions of the alkaline phosphatase gene of E. coli was constructed. The bovine growth hormone gene expression as well as protein partial processing and secretion into the periplasm have been shown to take place under phosphate starvation, i.e. conditions of alkaline phosphatase derepression.
authors.forename: I M
authors.initials: IM
authors.lastname: Tsfasman
authors.forename: V G
authors.initials: VG
authors.lastname: Nesmeianova
authors.forename: V G
authors.initials: VG
authors.lastname: Gorbulev
authors.forename: P M
authors.initials: PM
authors.lastname: Rubtsov
authors.forename: K G
authors.initials: KG
authors.lastname: Skriabin
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics*; D000818:Animals; D002417:Cattle; D002458:Cell Fractionation; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D005809:Genes, Regulator; D005822:Genetic Vectors; D013006:Growth Hormone / Q000096:biosynthesis* / Q000378:metabolism; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D013006:Growth Hormone; D000469:Alkaline Phosphatase
vernacular_title: Biosintez i sekretsiia gormona rosta byka u Escherichia coli pod kontrolem sekretornogo vektora soderzhashchego promotor i signal'nuiu posledovatel'nost' gena shchelochnoĭ fosfatazy.