Two-dimensional crystals of the photosystem II reaction center complex from higher plants.
By detergent treatment of isolated photosynthetic membranes from maize chloroplasts, we have prepared two-dimensional crystals of the photosystem II complex. Two distinct crystal forms are produced by this treatment. Analysis of Fourier transforms of the crystals shows that each crystal type is formed from two inverted layers. Within the rectangular 17.8 x 26.7 nm unit cell of each layer is a tetrameric structure enclosing a two-fold symmetry axis, a result implying that the basic structural unit of photosystem II is dimeric. Tris-washing, which removes proteins associated with the oxygen-evolving apparatus from the inner surface of the photosynthetic membrane, causes a distinct change in the structure of these tetramers and reveals a dimeric core complex which may be directly associated with the photosystem II machinery.
Bassi
R
R
Dipartimento di Biologia, Università di Padova, Italy.
Ghiretti Magaldi
A
A
Tognon
G
G
Giacometti
G M
GM
Miller
K R
KR
D002734:Chlorophyll; D003460:Crystallization; D005583:Fourier Analysis; D007091:Image Processing, Computer-Assisted; D015151:Immunoblotting; D045342:Light-Harvesting Protein Complexes; D008854:Microscopy, Electron; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D014325:Tromethamine; D003313:Zea mays
D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D014325:Tromethamine; D002734:Chlorophyll