A study of the conformation of 5S RNA by 31P NMR.
Only a small number of resolved, single phosphorous, phosphodiester resonances are observed in the 31P spectrum of the 5S rRNA from E. coli. Its spectrum is much simpler than that of a tRNA (Gueron, M. and Shulman, R.G. (1975) Proc. Natl. Acad. Sci. 72, 3482-3484), which suggests that 5S RNA does not have a tightly folded, tRNA-like, tertiary structure. The resolved resonances in the 5S spectrum originate in loops D and E, near bases 88 and 76, respectively.
Zhang
P
P
Department of Chemistry, Yale University, New Haven 06511.
Rycyna
R
R
Moore
P B
PB
D001483:Base Sequence; D002413:Cations, Divalent; D004926:Escherichia coli / Q000648:ultrastructure; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012335:RNA, Ribosomal; D012341:RNA, Ribosomal, 5S / Q000648:ultrastructure*
D002413:Cations, Divalent; D012335:RNA, Ribosomal; D012341:RNA, Ribosomal, 5S