Synthesis of hepatitis B virus e antigen in E. coli.
Hepatitis B virus core antigen (HBcAg) gene was deleted at some unique restriction enzyme sites, or at random, and inserted into the expression plasmids of E. coli which had the tryptophan promoter. E. coli transformants with the plasmids, synthesized materials with many kinds of antigenicity of HBcAg, HBeAg, or both HBcAg and HBeAg. HBeAg-specific material smaller than native HBeAg was produced in a stable condition.
Inada
T
T
Nihon Pharmaceutical Company, Research Division, Tokyo, Japan.
Misumi
Y
Y
Seno
M
M
Kanezaki
S
S
Shibata
Y
Y
Oka
Y
Y
Onda
H
H
D000595:Amino Acid Sequence; D000914:Antibodies, Viral; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D005814:Genes, Viral; D005822:Genetic Vectors; D006512:Hepatitis B Core Antigens / Q000235:genetics; D006513:Hepatitis B e Antigens / Q000235:genetics*; D006515:Hepatitis B virus / Q000235:genetics; D007120:Immunochemistry; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D014170:Transformation, Genetic
D000914:Antibodies, Viral; D006512:Hepatitis B Core Antigens; D006513:Hepatitis B e Antigens