Studies into hormonal imprinting in intact and transformed 3T3 fibroblast cell lines.
The cells of the NIH 3T3 fibroblast line responded to primary interaction with insulin by a positive imprinting, i.e. by an increased binding capacity for the hormone on re-exposure. Positive imprinting, although to a lesser degree, was also induced by thyrotropin. However, oncogenic transformation by polyoma virus oncogens resulted in decreased imprinting in both the middle-T-antigen (MT3) and small-T-antigen-expressing (N4) cells.
Kovács
P
P
Department of Biology, Semmelweis University of Medicine, Budapest, Hungary.
Okano
Y
Y
Nozawa
Y
Y
Csaba
G
G
D000952:Antigens, Polyomavirus Transforming / Q000502:physiology*; D002460:Cell Line; D002461:Cell Line, Transformed; D007328:Insulin / Q000378:metabolism / Q000502:physiology; D008856:Microscopy, Fluorescence; D011485:Protein Binding; D013972:Thyrotropin / Q000378:metabolism / Q000502:physiology; D015854:Up-Regulation / Q000502:physiology*
D000952:Antigens, Polyomavirus Transforming; D007328:Insulin; D013972:Thyrotropin