Cytogenetic response to coffee in Chinese hamster ovary AUXB1 cells and human peripheral lymphocytes.
We have investigated the genotoxic effects of three different brands and three types of coffee (freshly brewed regular, instant regular and freshly brewed decaffeinated) in two mammalian systems: the Chinese hamster ovary (CHO) AUXB1 cell line and human peripheral lymphocytes. Sister-chromatid exchanges (SCEs) and endoreduplicated cells (ERCs) were used as the endpoints. Coffee was prepared according to the manufacturer's suggestions, and after cooling, administered to cultured cells at dilutions ranging up to 11% that of full-strength coffee. Each brand and type of coffee induced significant levels of SCEs and ERCs in AUXB1 cells. SCEs, but not ERCs, were induced in human peripheral lymphocytes. Bisulfite, which complexes with carbonyls and reduces their genotoxicity, significantly diminished the number of SCEs and ERCs found after administration of coffee. Catalase and peroxidase, enzymes that destroy hydrogen peroxide activity, had no significant effect upon the SCE and ERC frequencies in AUXB1 cultures treated with freshly brewed regular coffee. These experiments indicate that different brands and types of coffee have sufficient genotoxic activity to increase SCEs and ERCs at levels only a fraction of those normally consumed. 1,2-Dicarbonyls alone and peroxides alone do not appear to be responsible for the majority of SCEs and ERCs that were observed to be induced by dilute coffee.
Tucker
J D
JD
Lawrence Livermore National Laboratory, Biomedical Sciences Division, University of California, Livermore 94550.
Taylor
R T
RT
Christensen
M L
ML
Strout
C L
CL
Hanna
M L
ML
D000447:Aldehydes; D000818:Animals; D002374:Catalase / Q000494:pharmacology; D002478:Cells, Cultured; D003069:Coffee / Q000633:toxicity*; D006224:Cricetinae; D004247:DNA / Q000187:drug effects; D005260:Female; D006037:Glyoxal; D006735:Horseradish Peroxidase / Q000494:pharmacology; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure; D009152:Mutagenicity Tests; D009153:Mutagens; D011765:Pyruvaldehyde; D012854:Sister Chromatid Exchange; D013447:Sulfites / Q000494:pharmacology
D000447:Aldehydes; D003069:Coffee; D009153:Mutagens; D013447:Sulfites; D006037:Glyoxal; D011765:Pyruvaldehyde; D004247:DNA; D006735:Horseradish Peroxidase; D002374:Catalase; C009279:sodium bisulfite