Evaluation and clinical applications of measurement of urinary growth hormone in diabetic subjects.
Quantities of growth hormone (GH) excreted into the urine over 24 h were measured by the highly sensitive sandwich enzyme immunoassay in 63 non-insulin-dependent diabetes mellitus (NIDDM) subjects, 6 insulin-dependent diabetes mellitus (IDDM) subjects, and 17 age-matched nondiabetic control subjects. GH-provocative tests with intravenous infusion of arginine revealed that urinary GH levels are closely correlated with the integrated concentrations of serum GH (r = .931, n = 14, P less than .001). Furthermore, 24-h urinary GH in control and diabetic subjects was inversely related to body mass index (r = .359, n = 80, P less than .001). The mean 24-h urinary GH in NIDDM subjects was 11.1 +/- 1.9 ng/g creatinine (Cr), which was not significantly different from that in nondiabetic control subjects (9.2 +/- 2.7 ng/g Cr). By contrast, the individual values for IDDM subjects varied widely, and their mean values (42.5 +/- 20.8 ng/g Cr) were much greater than those in the control and NIDDM subjects (P less than .01). The degree of glycemic control does not seem to affect 24-h urinary GH in NIDDM. The mean 24-h urinary GH in 7 subjects with proliferative diabetic retinopathy was comparable to that in subjects without retinopathy or with background retinopathy. Thus, the measurement of 24-h urinary GH appears to provide reliable assessments of endogenous GH secretion under physiological conditions and will be a useful tool for obtaining further insight into the role of GH in diabetes.
Suzuki
K
K
Division of Endocrinology and Metabolism, Institute for Adult Diseases, Asahi Life Foundation, Tokyo, Japan.
Miyata
H
H
Suzuki
T
T
Kajinuma
H
H
D000328:Adult; D000367:Age Factors; D000368:Aged; D001120:Arginine; D015415:Biomarkers / Q000652:urine; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000652:urine*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000652:urine*; D003930:Diabetic Retinopathy / Q000097:blood / Q000652:urine; D013006:Growth Hormone / Q000097:blood / Q000378:metabolism / Q000652:urine*; D006801:Humans; D007124:Immunoenzyme Techniques; D008875:Middle Aged; D012016:Reference Values
D015415:Biomarkers; D013006:Growth Hormone; D001120:Arginine