Beta-very low density lipoprotein pretreatment of endothelial monolayers increases monocyte adhesion.
Treatment of rabbit aortic endothelial cells or human umbilical vein cells for as little as 1 day with 25 micrograms/ml of beta-migrating very low density lipoprotein (beta-VLDL), but not low density lipoprotein (LDL), caused an increased binding of human peripheral blood monocytes to the endothelium. This increase was maximal by 24 hours but was not significant at 4 hours of pre-incubation with beta-VLDL. Neutrophil binding was not significantly stimulated by beta-VLDL treatment of endothelial cells, while endotoxin (LPS) treatment of endothelial cells stimulated both neutrophil and monocyte binding. Antibody to leukocyte function-associated-antigen-1 and to Mo2 inhibited binding to both beta-VLDL-stimulated and LPS-stimulated cells by 25%. The fact that both rabbit and human cells were stimulated by beta-VLDL to bind human monocytes suggests that some mechanisms regulating binding are conserved between species. These studies suggest that beta-VLDL acts like a chronic inflammatory mediator to cause a sustained increase in binding of monocytes to the endothelium.
Territo
M C
MC
Department of Medicine, University of California Los Angeles School of Medicine 90024.
Berliner
J A
JA
Almada
L
L
Ramirez
R
R
Fogelman
A M
AM
D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects*; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D007158:Immunologic Techniques; D066298:In Vitro Techniques; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D008079:Lipoproteins, VLDL / Q000494:pharmacology*; D016169:Lymphocyte Function-Associated Antigen-1; D009000:Monocytes / Q000166:cytology*; D009504:Neutrophils / Q000166:cytology; D011817:Rabbits; D016028:Receptors, Leukocyte-Adhesion / Q000378:metabolism; D013997:Time Factors
D000943:Antigens, Differentiation; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D016169:Lymphocyte Function-Associated Antigen-1; D016028:Receptors, Leukocyte-Adhesion