Incorporation of ionic channels from yeast plasma membranes into black lipid membranes.
Recently, patch-clamping of yeast protoplasts has revealed the presence of plasma membrane K+ channels (Gustin, M. C., B. Martinac, Y. Saimi, M. R. Culberston, and C. Kung. 1986. Science (Wash. DC). 233:1195-1197). In this work we show that fusion of purified plasma membranes into planar bilayers allows the study of the yeast channels. The main cationic conductances detected were of 64 and 116 pS, however, larger and smaller conductances have been observed. The two main conductances were sensitive to the K+ channels blockers tetraethylammonium (TEA+) and Ba2+. Bionic experiments indicated that both conductances were K+ selective.
Gómez-Lagunas
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Instituto de Fisiología Celular, Universidad Nacional Autónoma de México.
Peña
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Liévano
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Darszon
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D002462:Cell Membrane / Q000502:physiology; D008051:Lipid Bilayers; D008564:Membrane Potentials; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010718:Phosphatidylserines; D015221:Potassium Channels / Q000502:physiology*; D011523:Protoplasts / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000502:physiology*
D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010718:Phosphatidylserines; D015221:Potassium Channels; C021016:1,2-diphytanoylphosphatidylcholine