title: Single crystals of bacteriophage T7 RNA polymerase.
abstract: Single crystals of T7 RNA polymerase have been grown to a maximum size of 1.8 x 0.3 x 0.3 mm. The crystals are composed of fully intact T7 RNA polymerase which is enzymatically active upon dissolution. These crystals belong to the monoclinic space group P2(1) and have unit cell parameters a = 114.5 A, b = 139.6 A, c = 125.7 A, and beta = 98.1 degrees. Self-rotation function studies indicate that there are three molecules per asymmetric unit. The crystals diffract to at least 3.0 A resolution. These are the first crystals of a DNA-dependent RNA polymerase suitable for high-resolution X-ray structure determination.
authors.affiliation: Department of Crystallography, University of Pittsburgh, Pennsylvania 15260.
authors.forename: R
authors.initials: R
authors.lastname: Sousa
authors.forename: J P
authors.initials: JP
authors.lastname: Rose
authors.forename: Y J
authors.initials: YJ
authors.lastname: Chung
authors.forename: E M
authors.initials: EM
authors.lastname: Lafer
authors.forename: B C
authors.initials: BC
authors.lastname: Wang
mesh_terms: D003460:Crystallization; D012321:DNA-Directed RNA Polymerases / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology*; D008970:Molecular Weight; D011487:Protein Conformation; D013604:T-Phages / Q000201:enzymology*; D014961:X-Ray Diffraction
chemical_list: D012321:DNA-Directed RNA Polymerases