Schwann cells influence the expression of ganglioside GD3 by rat dorsal root ganglion neurons.
Cultured rat dorsal root ganglion neurons expressed ganglioside GD3 when grown in the absence of non-neuronal cells. Among the non-neuronal cells, fibroblasts, but not Schwann cells, also stained for ganglioside GD3 during the first few days in culture. When neurons were combined with non-neuronal cells the intensity of the GD3 immunoreactive neuronal processes was diminished at sites contacted by Schwann cells. This contact-mediated effect was specific for ganglioside GD3 since no difference was seen with A2B5 or JONES antibodies, which recognize different gangliosides.
Levison
S W
SW
Curriculum in Neurobiology, University of North Carolina, Chapel Hill 27599.
McCarthy
K D
KD
D000818:Animals; D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism*; D005732:Gangliosides / Q000032:analysis*; D009474:Neurons / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012583:Schwann Cells / Q000378:metabolism*; D012584:Sciatic Nerve / Q000166:cytology
D000911:Antibodies, Monoclonal; D005732:Gangliosides; C026226:ganglioside, GD3