Differential repair of UV damage in Saccharomyces cerevisiae.
Preferential repair of UV-induced damage is a phenomenon by which mammalian cells might enhance their survival. This paper presents the first evidence that preferential repair occurs in the lower eukaryote Saccharomyces cerevisiae. Moreover an unique approach is reported to compare identical sequences present on the same chromosome and only differing in expression. We determined the removal of pyrimidine dimers from two identical alpha-mating type loci and we were able to show that the active MAT alpha locus is repaired preferentially to the inactive HML alpha locus. In a sir-3 mutant, in which both loci are active this preference is not observed.
Terleth
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Leiden University, Department of Molecular Genetics, The Netherlands.
van Sluis
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CA
van de Putte
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P
D004249:DNA Damage; D004260:DNA Repair; D004271:DNA, Fungal / Q000302:isolation & purification / Q000528:radiation effects*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005800:Genes, Fungal / Q000528:radiation effects; D009154:Mutation; D011740:Pyrimidine Dimers / Q000528:radiation effects; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000528:radiation effects; D014158:Transcription, Genetic; D014466:Ultraviolet Rays
D004271:DNA, Fungal; D011740:Pyrimidine Dimers; C031851:endodeoxyribonuclease HaeII; D015252:Deoxyribonucleases, Type II Site-Specific