title: Phenotypic characterization of mononuclear white cells using finger-prick blood and light microscopy.
abstract: A technique for the enumeration of T cell subsets in capillary blood for use in the field is described. Mononuclear white cells were separated from 0.4 ml capillary blood, kept covered by culture medium on a Multitest slide, and incubated with monoclonal antibodies to the CD2, CD4, CD8, and interleukin-2 receptor antigens. Secondary and tertiary alkaline phosphatase conjugates were used for labeling. The substrate gave a distinct blue surface staining to the positive lymphocytes. Comparison was made with a conventional immunofluorescent antibody technique (r = 0.95).
authors.affiliation: University of Stockholm, Sweden.
authors.forename: L
authors.initials: L
authors.lastname: Smedman
authors.forename: M
authors.initials: M
authors.lastname: Troye-Blomberg
authors.forename: H
authors.initials: H
authors.lastname: Perlmann
authors.forename: A
authors.initials: A
authors.lastname: Naucler
authors.forename: P
authors.initials: P
authors.lastname: Perlmann
mesh_terms: D000328:Adult; D001402:B-Lymphocytes / Q000166:cytology; D001815:Bloodletting / Q000379:methods*; D002675:Child, Preschool; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D007958:Leukocyte Count / Q000379:methods; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D010641:Phenotype; D013601:T-Lymphocytes / Q000145:classification / Q000166:cytology