Dystrophin in skeletal muscle. I. Western blot analysis using a monoclonal antibody.
The value of analysing dystrophin on Western blots of skeletal muscle for the differential diagnosis of Xp21 muscular dystrophies is now fairly well established. Here we describe a sensitive system based on monoclonal antibodies to dystrophin. The specificity of the antibodies was established and experiments were undertaken to identify the source of dystrophin-related protein bands which were detected on blots of normal skeletal muscle. These investigations formed a necessary preliminary study to the application of the assay to samples of muscle obtained at biopsy from patients with Duchenne and Becker muscular dystrophy.
Nicholson
L V
LV
Muscular Dystrophy Group Research Laboratory, Newcastle General Hospital, Newcastle upon Tyne, U.K.
Davison
K
K
Falkous
G
G
Harwood
C
C
O'Donnell
E
E
Slater
C R
CR
Harris
J B
JB
D000911:Antibodies, Monoclonal; D001706:Biopsy; D015153:Blotting, Western; D016189:Dystrophin; D006801:Humans; D008970:Molecular Weight; D009124:Muscle Proteins / Q000276:immunology* / Q000302:isolation & purification; D009132:Muscles / Q000378:metabolism*; D009136:Muscular Dystrophies / Q000175:diagnosis* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D016189:Dystrophin; D009124:Muscle Proteins