Studies of whole blood associated acetaldehyde as a marker for alcohol intake in mice.
Thirty C57BI mice were randomized into two groups. Group 1 served as controls while Group 2 was given 10% V/V ethanol with the drinking water. Whole blood- associated acetaldehyde (WBAA) was measured on capillary blood samples using a fluorigenic high performance chromatographic assay. WBAA peaked at Day 2. A stable mean plateau of 263 +/- 71 SD with a range of 160-400 nmoles/g hemoglobin WBAA was found in the group consuming ethanol compared with 122 +/- 17 SD and a range of 88-150 nmoles/g hemoglobin for controls (p less than 0.001). When ethanol was discontinued, levels of WBAA declined and became similar to those of controls by 9 days following cessation of ethanol. The quantitative difference between ethanol-consuming and control animals and also the rapid rise of whole blood-associated acetaldehyde and the relatively slow decline following cessation of ethanol intake indicate that such a test might be a useful monitor of drinking behavior.
Peterson
C M
CM
Sansum Medical Research Foundation, Santa Barbara, California 93105.
Scott
B K
BK
D000079:Acetaldehyde / Q000097:blood*; D000428:Alcohol Drinking; D000818:Animals; D015415:Biomarkers / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D051379:Mice; D008810:Mice, Inbred C57BL; D011897:Random Allocation
D015415:Biomarkers; D000079:Acetaldehyde