[Synthesis of human proinsulin in Escherichia coli cells].
Expression of the synthetic gene for human proinsulin in E. coli has been investigated. The proinsulin gene has been expressed directly under the control of a synthetic promoter of phage fd DNA and a promoter of tryptophan operon, or using fusions with fragments of some bacterial proteins. These fusions gave insoluble polypeptide products amounting to 20-30% of total cellular protein. The scheme for isolating proinsulin from bacterial cells was developed. Proinsulin was cleaved from leader polypeptides by treatment with cyanogen bromide and converted into human insulin.
Efimov
V A
VA
Aleksiuk
I V
IV
Buriakova
A A
AA
Pashkova
I N
IN
Skiba
N P
NP
Chakhmakhcheva
O G
OG
D001435:Bacteriophages / Q000235:genetics; D002850:Chromatography, Gel; D003488:Cyanogen Bromide; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D015967:Gene Expression Regulation, Viral; D006801:Humans; D010957:Plasmids; D011384:Proinsulin / Q000096:biosynthesis* / Q000235:genetics; D011401:Promoter Regions, Genetic; D011863:Radioimmunoassay; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
D011994:Recombinant Proteins; D011384:Proinsulin; D003488:Cyanogen Bromide
Sintez proinsulina cheloveka v kletkakh Escherichia coli.