Effects of the neutrophil-activating peptide NAP-2, platelet basic protein, connective tissue-activating peptide III and platelet factor 4 on human neutrophils.
Platelet basic protein (PBP), connective tissue-activating peptide III (CTAP-III), and platelet factor 4 (PF-4) were purified from human platelet release supernatants by heparin-Sepharose ion-exchange and reversed-phase HPLC, and their neutrophil-activating effects were compared with those of NAP-2, a peptide of 70 amino acids corresponding to part of the sequence of PBP (1) and with sequence homology to NAF/NAP-1. NAP-2-induced elastase release and a rise in cytosolic free Ca2+ at concentrations between 0.3 and 100 nM, and neutrophil chemotaxis at concentrations between 0.03 and 10 nM. It was half as potent as NAF/NAP-1 in inducing exocytosis but showed the same activity in the other responses. By contrast, only minimal if any effects were obtained with PBP, CTAP-III, and PF-4 up to 100 nM. NAP-2 thus appears to behave like a typical chemotactic receptor agonist. It could be generated from PBP and/or CTAP-III released from activated platelets and lead to the accumulation of neutrophils in platelet aggregates.
Walz
A
A
Theodor-Kocher-Institut, University of Bern, Switzerland.
Dewald
B
B
von Tscharner
V
V
Baggiolini
M
M
D000595:Amino Acid Sequence; D002118:Calcium / Q000502:physiology; D018925:Chemokines; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D003600:Cytosol / Q000502:physiology; D005089:Exocytosis / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D016209:Interleukin-8; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000187:drug effects*; D010196:Pancreatic Elastase / Q000378:metabolism; D010455:Peptides / Q000494:pharmacology*; D010978:Platelet Factor 4 / Q000494:pharmacology*; D011506:Proteins / Q000494:pharmacology*; D001620:beta-Thromboglobulin
D018925:Chemokines; D016209:Interleukin-8; C061615:PPBP protein, human; D010455:Peptides; D011506:Proteins; D001620:beta-Thromboglobulin; D010978:Platelet Factor 4; C009862:connective tissue-activating peptide; D010196:Pancreatic Elastase; D002118:Calcium