title: Rapid homogeneous enzyme immunoassay of plasma protein C.
abstract: A homogeneous EIA (H-EIA) technique for plasma protein C antigen using horseradish peroxidase (HRP) was developed. This technique, which avoids the bound/free separation and washing procedures and shortens the assay time to 30 min, has several advantages over the conventional EIA technique. The measurement was valid for 1.0% to 200% of the plasma protein C level and had intraassay coefficients of variation of 2.3-11.0%. The results of this assay correlated well with those of conventional sandwich EIA (x) with a regression equation of y = 0.782x + 6.637, r = 0.911 (n = 59). This method, which may also be applicable to automatic assay systems, is considered to be suited for clinical laboratory use.
authors.affiliation: Department of Laboratory Medicine, Mie University School of Medicine, Tsu, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: T
authors.initials: T
authors.lastname: Hayashi
authors.forename: M
authors.initials: M
authors.lastname: Koyama
authors.forename: T
authors.initials: T
authors.lastname: Yoshimura
authors.forename: M
authors.initials: M
authors.lastname: Shimamoto
authors.forename: N
authors.initials: N
authors.lastname: Kimura
authors.forename: M
authors.initials: M
authors.lastname: Kita
mesh_terms: D000941:Antigens / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D008107:Liver Diseases / Q000097:blood; D011486:Protein C / Q000032:analysis* / Q000276:immunology; D012016:Reference Values; D015203:Reproducibility of Results; D013924:Thrombophlebitis / Q000097:blood; D014859:Warfarin / Q000494:pharmacology
chemical_list: D000941:Antigens; D011486:Protein C; D014859:Warfarin