Cellular localization of heat shock gene expression in rabbit cerebellum by in situ hybridization with plastic-embedded tissue.
The major cell types in rabbit cerebellum which engage in the expression of a heat shock gene (hsp70) after hyperthermia were identified. This required in situ hybridization on thin sections derived from plastic-embedded tissue. All classes of cerebellar neurons which were examined (Purkinje, granule, and stellate cells) responded by induction of hsp70 mRNA within 1 hr after hyperthermia. Prominent induction of hsp70 mRNA was also observed in oligodendroglia in the deep white matter.
Masing
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Department of Zoology, University of Toronto, West Hill, Ontario, Canada.
Brown
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D000818:Animals; D001345:Autoradiography; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism*; D010450:Endopeptidases; D015870:Gene Expression; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D009474:Neurons / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010969:Plastics; D011689:Purkinje Cells / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D012260:Ribonucleases
D006360:Heat-Shock Proteins; D010969:Plastics; D012333:RNA, Messenger; D012260:Ribonucleases; D010450:Endopeptidases