[DNA polymerase I, Klenow fragment of Escherichia coli: hydrolysis and pyrophosphorolysis of DNA containing phosphothioate groups].
Reaction of DNA synthesis catalyzed by DNA polymerase I KF in the presence of 2'-deoxynucleoside 5'-alpha-thiotriphosphates (dNTP alpha S) was investigated. DNA with thiophosphate groups (DNA[P=S]) obtained by such a way was studied in reactions of hydrolysis and pyrophosphorolysis catalyzed by DNA polymerase I KF. It is shown that the rate of DNA elongation is decreased both on the step of incorporation of dNMP alpha S residues and on the step of incorporation of the next dNMP residue. The rate of pyrophosphorolysis of 3'-terminal dNMP alpha S was demonstrated to be one order of magnitude less in comparison with the corresponding reaction with the natural dNMP residue. Contrary, the rate of 3'----5'-exonuclease hydrolysis of both DNA[P=S] and DNA of the same structure revealed no distinguishable differences.
Rozovskaia
T A
TA
Minasian
Sh Kh
ShKh
Kukhanova
M K
MK
Kraevskiĭ
A A
AA
Chidzhavadze
Z G
ZG
D001435:Bacteriophages / Q000235:genetics; D001483:Base Sequence; D002845:Chromatography; D004256:DNA Polymerase I / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology*; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009946:Organothiophosphorus Compounds / Q000378:metabolism; D010766:Phosphorylation
D004279:DNA, Viral; D009946:Organothiophosphorus Compounds; D004256:DNA Polymerase I; D004259:DNA-Directed DNA Polymerase
DNK-polimeraza I Escherichia coli, fragment Klenova: gidroliz i pirofosforoliz DNK, soderzhashcheĭ fosfotioatnye gruppy.