virG of Agrobacterium tumefaciens plasmid pTiC58 encodes a DNA-binding protein.
Virulence genes of the Agrobacterium tumefaciens Ti plasmid are positively regulated by the products of virA and virG. To study the DNA-binding properties of the VirG protein, a translational fusion between virG and the trpE gene of Escherichia coli was constructed, and antiserum was raised against the encoded fusion protein. Using this antiserum, a protein of Mr congruent to 29,000, a size similar to that calculated from the virG nucleotide sequence, was detected in an E. coli strain harbouring a virG expression vector. Both the virG protein and the fusion protein were found, by filter-binding and gel retardation analyses, to bind DNA nonspecifically. These data support an existing model for the two-component regulatory systems of bacteria.
Powell
B S
BS
Department of Plant Pathology, University of California, Davis 95616.
Rogowsky
P M
PM
Kado
C I
CI
D000907:Antibodies, Bacterial / Q000276:immunology; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D004586:Electrophoresis / Q000379:methods; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D005809:Genes, Regulator; D008954:Models, Biological; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D015183:Restriction Mapping; D012231:Rhizobium / Q000235:genetics* / Q000472:pathogenicity; D014364:Tryptophan / Q000235:genetics; D014774:Virulence
D000907:Antibodies, Bacterial; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D011994:Recombinant Proteins; D014364:Tryptophan