Simultaneous detection of Escherichia coli heat-stable and heat-labile enterotoxin genes with a single RNA probe.
A single RNA probe was synthesized and used to detect simultaneously the methanol-soluble heat-stable enterotoxin and heat-labile enterotoxin genes in Escherichia coli strains. The results with the biotinylated or radioactive probe correlated 100% with the biological assay results for both toxins. The RNA probe detected the three known heat-stable enterotoxin A alleles.
Sáez-Llorens
X
X
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235.
Guzman-Verduzco
L M
LM
Shelton
S
S
Nelson
J D
JD
Kupersztoch
Y M
YM
D000483:Alleles; D001427:Bacterial Toxins / Q000235:genetics*; D001710:Biotin; D004269:DNA, Bacterial / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D004768:Enterotoxins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D006801:Humans; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D015347:RNA Probes; D015183:Restriction Mapping
D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D015347:RNA Probes; C034151:heat stable toxin (E coli); D001710:Biotin; C027538:heat-labile enterotoxin, E coli