[Chemical characterization of Escherichia coli K 1 capsular polysaccharide].
The exact chemical characterization of the prepared Escherichia coli K 1 capsule polysaccharide is necessary and a prerequisite for using this antigen as a screening antigen in the production of monoclonal anti-K 1-antibodies. The K 1-antigen, prepared by phenol-water-extraction, was analyzed by protein, RNA, and neuraminic acid determination. An addition, the antigen was subjected to elementary analysis, infrared- and 13C-NMR-spectroscopy, and gelchromatography. After testing the serological specificity, the K 1-antigen was identified as a high molecular polyneuraminic acid.
Mehl
M
M
Institut für Medizinische Mikrobiologie des Bereiches Medizin, Humboldt-Universität zu Berlin.
Starke
R
R
Kissig
B
B
Grunow
R
R
Jahn
S
S
Schmidt
G
G
von Baehr
R
R
D000942:Antigens, Bacterial / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D004926:Escherichia coli / Q000032:analysis* / Q000276:immunology; D011135:Polysaccharides, Bacterial / Q000032:analysis* / Q000276:immunology
D000942:Antigens, Bacterial; D000954:Antigens, Surface; C030659:K antigens; D011135:Polysaccharides, Bacterial
Chemische Charakterisierung des Escherichia coli K 1 Kapselpolysaccharides.