title: Nucleotide sequence of a cDNA coding for the NADPH-protochlorophyllide oxidoreductase (PCR) of barley (Hordeum vulgare L.) and its expression in Escherichia coli.
abstract: The primary structure of the NADPH-protochlorophyllide oxidoreductase of barley has been deduced from the nucleotide sequence of a cloned full-length cDNA. This cDNA hybridizes to a 1.7 kb RNA whose steady-state level in dark-grown seedlings is drastically reduced upon illumination. The predicted amino acid sequence (388 residues in length) includes a transit peptide of 74 amino acids whose end point has been delimited by sequencing the N-terminus of the mature protein. Expression of the cDNA in Escherichia coli leads to the synthesis of an enzymatically active precursor of the NADPH-protochlorophyllide oxidoreductase. Activity of this protein in bacterial lysates is completely dependent on the presence of NADPH and protochlorophyllide and requires light.
authors.affiliation: Botanisches Institut, Christian-Albrechts-Universität Kiel, Federal Republic of Germany.
authors.forename: R
authors.initials: R
authors.lastname: Schulz
authors.forename: K
authors.initials: K
authors.lastname: Steinmüller
authors.forename: M
authors.initials: M
authors.lastname: Klaas
authors.forename: C
authors.initials: C
authors.lastname: Forreiter
authors.forename: S
authors.initials: S
authors.lastname: Rasmussen
authors.forename: C
authors.initials: C
authors.lastname: Hiller
authors.forename: K
authors.initials: K
authors.lastname: Apel
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics*; D044925:Oxidoreductases Acting on CH-CH Group Donors; D010944:Plants / Q000201:enzymology / Q000235:genetics*
chemical_list: D004247:DNA; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C023886:protochlorophyllide reductase