Changes in phospholipid and cholesterol concentrations of the rat microvillus membrane during maturation.
We studied the cholesterol and phospholipid content and the cholesterol-to-phospholipid molar ratio of the small intestinal microvillus membrane in fetal, weanling, and adult rats. We also investigated the effect of glucocorticoid administration on these lipids and the ontogeny of 1,2-diglycerol-CDP choline phosphocholine transferase (PCT). Cholesterol and phospholipid concentrations decreased with maturation, phospholipids declining more than cholesterol. Thus, the cholesterol-to-phospholipid molar ratio rose with maturation. A similar decline was seen with PCT activity. Glucocorticoid treatment had no effect on cholesterol, phospholipids, or PCT. However, the maturational increase in the cholesterol-to-phospholipid molar ratio was blunted by glucocorticoid administration in the weanling and adult rats.
Engelhardt
E L
EL
University of Florida College of Medicine, Department of Pediatrics, Gainesville 32610.
Neu
J
J
Sankar
M B
MB
Gimotty
P A
PA
Meyer
J W
JW
D000367:Age Factors; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D000831:Animals, Newborn; D002784:Cholesterol / Q000032:analysis*; D005325:Fetal Organ Maturity; D006854:Hydrocortisone / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000032:analysis*; D007421:Intestine, Small / Q000032:analysis / Q000187:drug effects / Q000254:growth & development*; D008297:Male; D008871:Microvilli / Q000032:analysis; D010743:Phospholipids / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D000520:alpha-Glucosidases / Q000032:analysis
D010743:Phospholipids; D002784:Cholesterol; D000469:Alkaline Phosphatase; D000520:alpha-Glucosidases; D006854:Hydrocortisone