title: Regulation of lymphocyte motility by macrophages: characterization of a lymphocyte migration inhibitory factor derived from a macrophage-like cell line.
abstract: An inhibitory factor on lymphocyte migration was detected using a capillary random migration assay in the culture supernatant of peritoneal exudate macrophages cultured at concentrations greater than 8 x 10(6) cells/ml. After examining different macrophage-like cell lines, J774A.1 cells were found to produce this inhibitory factor, which was termed lymphocyte migration inhibitory factor (LMIF). The inhibitory effect of LMIF on the migration of spleen lymphocytes, thymocytes, and bone marrow cells was determined. The migration of thymocytes was more sensitive to LMIF than was the migration of spleen lymphocytes and bone marrow cells. Interestingly, when the effect of LMIF was tested on the migration of spleen T cells and B cells, T cells were more sensitive than B cells. When the thymocytes were separated by peanut agglutinin into mature and immature thymocytes, the migration of mature thymocytes was more sensitive than that of immature thymocytes, the migration of mature thymocytes was more sensitive than that of immature thymocytes to the effect of LMIF, suggesting that the greatest effect of LMIF was on the migration of mature T cells. Partial purification of LMIF by ion-exchange and gel-filtration chromatography revealed that it is approximately 14,000 in molecular weight and could exist in either monomeric or dimeric forms. The possible role of this factor in an immune response is discussed.
authors.affiliation: Department of Biological Sciences, Illinois State University, Normal 61761.
authors.forename: S
authors.initials: S
authors.lastname: Ye
authors.forename: H T
authors.initials: HT
authors.lastname: Cheung
mesh_terms: D000818:Animals; D002460:Cell Line; D002465:Cell Movement; D007961:Leukocyte Migration-Inhibitory Factors / Q000032:analysis / Q000302:isolation & purification / Q000502:physiology*; D008214:Lymphocytes / Q000145:classification / Q000502:physiology*; D008222:Lymphokines / Q000502:physiology*; D008264:Macrophages / Q000502:physiology*; D051379:Mice
chemical_list: D007961:Leukocyte Migration-Inhibitory Factors; D008222:Lymphokines