title: The location of DNA in RecA-DNA helical filaments.
abstract: The helical filament that the RecA protein of Escherichia coli forms around DNA is the active apparatus in protein-catalyzed homologous genetic recombination. The actual position of DNA within this complex has been unknown. Image analysis has been performed on electron micrographs of filaments of RecA on double-stranded DNA and on single-stranded DNA to visualize a difference that is consistent with one strand of the double-stranded DNA. This localization of the DNA gives additional information about the unusual structure of DNA in the complex with RecA protein.
authors.affiliation: Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511.
authors.forename: E H
authors.initials: EH
authors.lastname: Egelman
authors.forename: X
authors.initials: X
authors.lastname: Yu
mesh_terms: D004269:DNA, Bacterial / Q000032:analysis* / Q000648:ultrastructure; D004277:DNA, Single-Stranded / Q000032:analysis* / Q000648:ultrastructure; D004926:Escherichia coli / Q000235:genetics*; D005583:Fourier Analysis; D007091:Image Processing, Computer-Assisted; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D011938:Rec A Recombinases / Q000032:analysis* / Q000648:ultrastructure; D011995:Recombination, Genetic
chemical_list: D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D046911:Macromolecular Substances; D011938:Rec A Recombinases