title: [Synthesis and expression of an artificial gene of an IgG-binding fragment of protein A from Staphylococcus aureus].
abstract: The chemical-enzymatic synthesis of a gene for IgG-binding fragment of the staphylococcal protein A has been carried out. The design of the gene, which consists of signal peptide and modified E and B domains, and strategy of the synthesis provided possibility of various degrees of polymerization of the gene fragment coding for B domain and of the whole gene. Several protein A-like polypeptides composed of the leader sequence, E domain and 1 to 4 copies of B domain were produced in E. coli cells under the lac promoter control.
authors.forename: V A
authors.initials: VA
authors.lastname: Efimov
authors.forename: A A
authors.initials: AA
authors.lastname: Buriakova
authors.forename: N N
authors.initials: NN
authors.lastname: Polushin
authors.forename: I N
authors.initials: IN
authors.lastname: Pashkova
authors.forename: E V
authors.initials: EV
authors.lastname: Dmitrakova
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005813:Genes, Synthetic; D007128:Immunoglobulin Fragments / Q000235:genetics*; D008969:Molecular Sequence Data; D013205:Staphylococcal Protein A / Q000235:genetics*; D013211:Staphylococcus aureus / Q000235:genetics* / Q000276:immunology
chemical_list: D007128:Immunoglobulin Fragments; D013205:Staphylococcal Protein A
vernacular_title: Sintez i ékspressiia iskusstvennogo gena IgG-sviazyvaiushchego fragmenta bela A Staphylococcus aureus.