title: The primary structure of porcine liver acylamino acid-releasing enzyme deduced from cDNA sequences.
abstract: Two overlapping cloned cDNAs encoding the entire amino acid sequences of the subunits of acylamino acid-releasing enzyme (AARE) [EC 3.4.19.1] have been isolated from porcine liver cDNA lambda gt10 cDNA libraries and sequenced. Sequence analyses of the cDNA and several Achromobacter protease I-digested peptides of the purified protein revealed that porcine liver AARE consists of four identical subunits, and each comprising a single chain of 732 amino acids with acetylmethionine at the N-terminus.
authors.affiliation: Biotechnology Research Laboratories, Takara Shuzo Co., Ltd., Shiga.
authors.forename: M
authors.initials: M
authors.lastname: Mitta
authors.forename: K
authors.initials: K
authors.lastname: Asada
authors.forename: Y
authors.initials: Y
authors.lastname: Uchimura
authors.forename: F
authors.initials: F
authors.lastname: Kimizuka
authors.forename: I
authors.initials: I
authors.lastname: Kato
authors.forename: F
authors.initials: F
authors.lastname: Sakiyama
authors.forename: S
authors.initials: S
authors.lastname: Tsunasawa
mesh_terms: D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases / Q000235:genetics*; D015183:Restriction Mapping; D013552:Swine
chemical_list: D004247:DNA; D010447:Peptide Hydrolases; C038220:acylaminoacyl-peptidase