title: Cooperative tandem binding of met repressor of Escherichia coli.
abstract: We present biochemical and genetic data to support the hypothesis that the Escherichia coli met repressor, MetJ, binds to synthetic and natural operator sequences in tandem arrays such that repression depends not only on the affinity of the DNA-protein interaction, but also on protein-protein contacts along the tandem array. This represents a novel form of regulatory switch. Furthermore, there seems to be homology between the organization of the met and trp operators.
authors.affiliation: Astbury Department of Biophysics, University of Leeds, UK.
authors.forename: S E
authors.initials: SE
authors.lastname: Phillips
authors.forename: I
authors.initials: I
authors.lastname: Manfield
authors.forename: I
authors.initials: I
authors.lastname: Parsons
authors.forename: B E
authors.initials: BE
authors.lastname: Davidson
authors.forename: J B
authors.initials: JB
authors.lastname: Rafferty
authors.forename: W S
authors.initials: WS
authors.lastname: Somers
authors.forename: D
authors.initials: D
authors.lastname: Margarita
authors.forename: G N
authors.initials: GN
authors.lastname: Cohen
authors.forename: I
authors.initials: I
authors.lastname: Saint-Girons
authors.forename: P G
authors.initials: PG
authors.lastname: Stockley
mesh_terms: D001059:Apoproteins / Q000096:biosynthesis / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism; D001483:Base Sequence; D004269:DNA, Bacterial / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009875:Operator Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D012091:Repetitive Sequences, Nucleic Acid; D012097:Repressor Proteins / Q000096:biosynthesis / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D014364:Tryptophan / Q000378:metabolism
chemical_list: D001059:Apoproteins; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C053317:MetJ protein, E coli; D012097:Repressor Proteins; D014157:Transcription Factors; D014364:Tryptophan