Purification and amino acid sequencing of naturally occurring N-formyl-methionyl oligopeptides from Escherichia coli.
A novel purification procedure for N-formyl methionyl oligopeptides from bacterial culture supernatants has been developed. C-terminal carboxypeptidase microsequencing of purified peptides from E. coli supernatants enabled identification of 6 naturally occurring formyl-oligopeptides.
Broom
M F
MF
Wellcome Medical Research Institute, University of Otago Medical School, Dunedin, New Zealand.
Mellor
D M
DM
Chadwick
V S
VS
D000595:Amino Acid Sequence; D002268:Carboxypeptidases; D004926:Escherichia coli / Q000378:metabolism*; D008715:Methionine / Q000031:analogs & derivatives; D008969:Molecular Sequence Data; D009239:N-Formylmethionine; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000302:isolation & purification; D009842:Oligopeptides / Q000302:isolation & purification*
D009842:Oligopeptides; D009239:N-Formylmethionine; D009240:N-Formylmethionine Leucyl-Phenylalanine; D008715:Methionine; D002268:Carboxypeptidases; C022633:serine carboxypeptidase