Cloning and nucleotide sequence of a flagellin-coding gene (hag) from Serratia marcescens 274.
The pigmented enterobacterium Serratia marcescens shows a high frequency of color variation. In strain 274, color variation was shown to be closely associated with flagellar variation [Paruchuri and Harshey, J. Bacteriol. 169 (1987) 61-65]. As a first step towards understanding the genetic basis of this phenomenon, we have cloned and sequenced a flagellin-coding gene of S. marcescens 274. The gene codes for a protein product of 351 aa, with a predicted Mr of 36,862. Amino acid sequence comparisons reveal a high degree of homology between the N-terminal half and C-terminal third of the Serratia flagellin and flagellins from Escherichia coli and Salmonella typhimurium.
Harshey
R M
RM
Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037.
Estepa
G
G
Yanagi
H
H
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005408:Flagellin / Q000096:biosynthesis / Q000235:genetics*; D005798:Genes, Bacterial; D014644:Genetic Variation; D008969:Molecular Sequence Data; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D012486:Salmonella typhimurium / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D012706:Serratia marcescens / Q000235:genetics*
D000596:Amino Acids; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D005408:Flagellin