Difference in effect of cultured fetal pancreas transplants on retinal and renal capillary basement membrane thickness in diabetic mice.
The goal of endocrine pancreas transplants should be the prevention of diabetic complications. The differential effect of grafts of organ-cultured fetal mouse pancreas on diabetic complications in the retina and kidney was tested by comparing capillary basement membrane thickness (BMT) in mice made diabetic with streptozotocin and transplanted either early or late, or treated with insulin. BALB/c female mice were grafted with a single organ-cultured syngeneic fetal pancreas at either 3 weeks or 7 months after induction of diabetes. Controls were sex- and age-matched nondiabetic; diabetic untreated; and diabetic insulin-treated mice. All mice were killed at 20 months of age and their eyes and kidneys fixed for electron microscopy. BMT was measured on coded micrographs. In all mice glomerular capillary BM were thicker than retinal capillary BM. Mice grafted early after the induction of diabetes had normal BMT in both sites, while those transplanted after 6 months of disease had normal retinal, but thickened glomerular, capillary BM. In each case the late-transplanted animals had BM thickness significantly less than the insulin-treated or untreated diabetics.
Cuthbertson
R A
RA
Walter and Eliza Hall Institute of Medical Research, Victoria, Australia.
Hopper
J L
JL
Mandel
T E
TE
D000818:Animals; D001485:Basement Membrane / Q000473:pathology*; D002196:Capillaries / Q000473:pathology*; D003921:Diabetes Mellitus, Experimental / Q000473:pathology / Q000628:therapy*; D007328:Insulin / Q000627:therapeutic use; D007678:Kidney Glomerulus / Q000098:blood supply / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010179:Pancreas / Q000196:embryology; D016035:Pancreas Transplantation; D012160:Retina / Q000098:blood supply / Q000473:pathology*
D007328:Insulin