Chronic imipramine reduces [3H]SCH 23390 binding and DA-sensitive adenylate cyclase in the limbic system.
[3H]SCH 23390 binding and dopamine (DA)-stimulated adenylate cyclase activity were measured in brain membrane preparations from rats chronically treated with imipramine (10 mg/kg twice daily for 14 days). [3H]SCH 23390 binding sites were decreased by 27% in the limbic system but only 14% in the striatum. The responsiveness of adenylate cyclase to DA was reduced by 38% in the limbic system but was not modified in the striatum. Concomitant treatment with alpha-methyltyrosine (alpha-MPT) (50 mg/kg daily for 14 days) prevented the imipramine-induced reduction in both [3H]SCH 23390 binding sites and the responsiveness of adenylate cyclase to DA.
De Montis
G M
GM
Institute of Pharmacology and Biochemical Pathology, University of Cagliari, Italy.
Devoto
P
P
Gessa
G L
GL
Meloni
D
D
Porcella
A
A
Saba
P
P
Serra
G
G
Tagliamonte
A
A
D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D001552:Benzazepines / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004298:Dopamine / Q000494:pharmacology*; D007099:Imipramine / Q000494:pharmacology*; D008032:Limbic System / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D008781:Methyltyrosines / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D019805:alpha-Methyltyrosine
D001552:Benzazepines; D008781:Methyltyrosines; D015102:3,4-Dihydroxyphenylacetic Acid; D019805:alpha-Methyltyrosine; D000262:Adenylyl Cyclases; D007099:Imipramine; D004298:Dopamine