title: In vitro growth properties and PCR ras gene analysis of a murine embryonal carcinoma cell line harboring an amplified c-Ki-ras gene.
abstract: Ras proto-oncogenes are thought to be involved in both proliferation and malignant processes. We examined some growth properties of a murine embryonal carcinoma cell line (ECC), PCC4, that have been shown previously to be amplified for the c-KI-ras gene. Our results show that doubling time and plating efficiency are not significantly affected by such amplification. To examine the possible link between malignant behavior and c-Ki-ras alteration, we investigated activating mutations in this PCC4 cell line as well as in other ECC. Analysis of the in vitro amplified Ki-ras gene by PCR technology has not revealed point mutations in any of the ECC examined.
authors.affiliation: UPR 43 CNRS Rétrovirus et Rétrotransposons des Vertébrés.
authors.forename: D
authors.initials: D
authors.lastname: Vilette
authors.forename: L
authors.initials: L
authors.lastname: d'Auriol
authors.forename: F
authors.initials: F
authors.lastname: Galibert
authors.forename: J
authors.initials: J
authors.lastname: Peries
authors.forename: R
authors.initials: R
authors.lastname: Emanoil-Ravier
mesh_terms: D000818:Animals; D002277:Carcinoma; D002455:Cell Division; D002460:Cell Line; D005784:Gene Amplification; D011905:Genes, ras; D051379:Mice; D009154:Mutation; D011519:Proto-Oncogenes; D014407:Tumor Cells, Cultured / Q000166:cytology*