Identification of a novel protein (G210) specific to the Golgi apparatus.
A monoclonal antibody, 3C9, has enabled the detection of a novel Golgi-specific protein in bovine tissues. Immunohistochemical studies at the light microscopic level have detected the 3C9 antigen only in certain cells: exocrine pancreas, gut epithelium, and thymus epithelium. Examination of gut and pancreas by immunoelectron microscopy showed a localization exclusive to the Golgi apparatus. The relative molecular weight of the antigen detected by immunoblotting is 210,000 daltons. The antigen is not extracted from microsomal membranes of bovine gut epithelium by sodium carbonate solutions. Furthermore, the 3C9 antigen enters into the detergent phase when Triton X-114 partitioning methods are used. These data strongly suggest that this novel antigen is an intrinsic membrane protein, resident in the Golgi apparatus of certain cells. Moreover, they enhance the hypothesis that the distribution of enzymes and polypeptides in the Golgi apparatus is cell specific.
Hogue-Angeletti
R
R
Department of Pathology & Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia 19104-6082.
Xu
R Y
RY
Gonatas
J O
JO
Stieber
A
A
Gonatas
N K
NK
D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D006056:Golgi Apparatus / Q000032:analysis*; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D007421:Intestine, Small / Q000032:analysis / Q000648:ultrastructure; D008854:Microscopy, Electron; D008970:Molecular Weight; D010179:Pancreas / Q000032:analysis / Q000648:ultrastructure; D011506:Proteins / Q000032:analysis*
D000911:Antibodies, Monoclonal; D011506:Proteins