title: Freeze-fracture studies on the sporoblast and sporozoite development in the early oocyst.
abstract: Freeze-fracturing has been used to study the formation of the triple layer pellicular complex of budding sporozoites of Plasmodium falciparum in the early oocyst. Sporozoites are formed from sporoblasts within the oocyst. The outer membrane of the sporozoites is derived from the single plasma membrane of the sporoblast while the inner 2 membranes are formed anew at the base of the differentiating sporozoites. A dense collar of intramembranous particles located on the P face of the outer membrane encircles the base of each budding sporozoite. The fact that this collar of intramembranous particles is located in the same region where the inner membranes of the sporozoites first make their appearance strongly suggests that the 2 are related, and that the collar may be related to either membrane synthesis or to membrane organization and assembly.
authors.affiliation: Naval Medical Research Institute, Bethesda, Maryland.
authors.forename: C A
authors.initials: CA
authors.lastname: Meszoely
authors.forename: E F
authors.initials: EF
authors.lastname: Erbe
authors.forename: L M
authors.initials: LM
authors.lastname: Beaudoin
authors.forename: R L
authors.initials: RL
authors.lastname: Beaudoin
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D003593:Cytoplasm / Q000648:ultrastructure; D005614:Freeze Fracturing; D008854:Microscopy, Electron; D010963:Plasmodium falciparum / Q000254:growth & development / Q000648:ultrastructure*