title: Enzyme catalyzed acylation and deacylation of the sugar moieties of nucleosides.
abstract: We have found that a lipase from Pseudomonas fluorescens (PFL) accelerated regioselective acylation of 2'-deoxynucleosides with the use of acid anhydrides as acyl donor in dry polar solvents. Different regioselective deacylation of 3',5'-di-O-acyl-2'-deoxynucleosides was found to take place when a lipase (PFL) or a protease from Bacillus subtilis (Subtilisin) was used.
authors.affiliation: Chemical Synthesis Research Laboratory, TAIHO Pharmaceutical Co., Ltd., Saitama, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nozaki
authors.forename: A
authors.initials: A
authors.lastname: Uemura
authors.forename: J
authors.initials: J
authors.lastname: Yamashita
authors.forename: M
authors.initials: M
authors.lastname: Yasumoto
mesh_terms: D000215:Acylation; D001711:Biotransformation; D008049:Lipase / Q000378:metabolism; D009705:Nucleosides / Q000378:metabolism*; D013381:Subtilisins / Q000378:metabolism
chemical_list: D009705:Nucleosides; D008049:Lipase; D013381:Subtilisins