Gene transfer into hemopoietic stem cells using retroviral vectors.
Gene transfer to hemopoietic cells offers a variety of new approaches to the experimental hematologist as well as potentially providing a means for correcting a number of genetic disorders of humans. From the experimental viewpoint, gene transfer utilizing retroviral vectors introduces new methods for analyzing hemopoietic cell lineages, and the effects of over-expression of genes affecting hemopoietic cell proliferation and differentiation. The unique properties of retroviral vectors and the optimized methods currently in use to infect hemopoietic cells are represented as a brief review of a rapidly expanding new field of experimental hematology.
Chang
J M
JM
Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia.
Johnson
G R
GR
D000818:Animals; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D051379:Mice; D012190:Retroviridae; D014162:Transfection