[Analysis of expression of the gene from the BRa puff of Chironomus thummi encoding the low molecular weight secretory protein].
The plasmid containing F6.2 gene from the BRa of Chironomus thummi within the pUR 292 vector was constructed. Chimeric protein containing beta-galactosidase-F6.2 polypeptide was produced in Escherichia coli BMH71-18. The protein obtained has immunological similarity with secretion protein of 67,000 D from Ch. thummi salivary gland. The gene for sp67 is active in main and in special lobe of the gland. Based on the data obtained and on the previous results of the authors, conclusion is made that BRa is a complex locus containing several types of genes.
Bogachev
S S
SS
Shcherbik
S V
SV
Taranin
A V
AV
Sebeleva
T E
TE
D000818:Animals; D001483:Base Sequence; D002683:Chironomidae / Q000235:genetics*; D004247:DNA / Q000235:genetics; D004175:Diptera / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005822:Genetic Vectors; D015151:Immunoblotting; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D012471:Salivary Proteins and Peptides / Q000235:genetics* / Q000378:metabolism
D010455:Peptides; D012471:Salivary Proteins and Peptides; D004247:DNA
Analiz ékspressii gena iz tkanespetsificheskogo pufa KBa Chironomus thummi, kodiruiushchego nizkomolekuliarnyĭ sekretornyĭ polipeptid.