ELISA for diagnosis of gnathostomiasis using antigens from Gnathostoma doloresi and G. spinigerum.
ELISA was developed for the detection of IgG antibody in sera obtained from patients in Japan and in a foreign country. Gnathostoma doloresi adult antigen was less specific than G. spinigerum advanced third-stage larval antigen but their sensitivity were similar. Cross reactivity was observed in Toxocara canis-, Anisakis-, Paragonimus westermani- and Fasciola-infected sera when G. doloresi adult worms but not G. spinigerum advanced third-stage larvae were used as antigens.
Anantaphruti
M T
MT
Department of Parasitology, Faculty of Medicine, Hiroshima University, Japan.
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006039:Gnathostoma / Q000276:immunology*; D006801:Humans; D009349:Nematode Infections / Q000175:diagnosis / Q000276:immunology*; D012680:Sensitivity and Specificity; D013802:Thelazioidea / Q000276:immunology*
D000947:Antigens, Helminth