Studies with crosslinking reagents on the oligomeric structure of the env glycoprotein of HIV.
The subunit composition of env glycoproteins isolated from HIV-infected cell membranes, virus particles, and baculovirus recombinant-infected insect cells has been investigated using a number of crosslinking reagents. The results obtained provide evidence that gp160 is a tetramer of identical subunits and that gp41 is also tetrameric.
Schawaller
M
M
Division of Virology, National Institute for Medical Research, Mill Hill, London, United Kingdom.
Smith
G E
GE
Skehel
J J
JJ
Wiley
D C
DC
D015153:Blotting, Western; D003432:Cross-Linking Reagents; D006678:HIV / Q000648:ultrastructure*; D007304:Insect Viruses; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins / Q000648:ultrastructure*; D008970:Molecular Weight; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins / Q000648:ultrastructure*
D003432:Cross-Linking Reagents; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins