title: Cloning and sequence analysis of the gene encoding invertase from the yeast Schwanniomyces occidentalis.
abstract: The gene encoding invertase (INV) has been cloned from Schwanniomyces occidentalis. The enzyme consists of 533 amino acids, 8 potential glycosylation sites and has a 45% identity with the invertase from Saccharomyces cerevisiae. The proenzyme has a 22 amino acid signal sequence that has a high alpha-helical transmembrane potential which differs significantly from that predicted for the Saccharomyces cerevisiae enzyme.
authors.affiliation: Molecular Biology Research, Upjohn Company, Kalamazoo, MI 49007.
authors.forename: R D
authors.initials: RD
authors.lastname: Klein
authors.forename: R A
authors.initials: RA
authors.lastname: Poorman
authors.forename: M A
authors.initials: MA
authors.lastname: Favreau
authors.forename: M H
authors.initials: MH
authors.lastname: Shea
authors.forename: N T
authors.initials: NT
authors.lastname: Hatzenbuhler
authors.forename: S C
authors.initials: SC
authors.lastname: Nulf
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon / Q000502:physiology; D004271:DNA, Fungal / Q000235:genetics; D006026:Glycoside Hydrolases / Q000235:genetics*; D008969:Molecular Sequence Data; D012045:Regulatory Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D004718:Saccharomycetales / Q000201:enzymology / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D043324:beta-Fructofuranosidase
chemical_list: D003062:Codon; D004271:DNA, Fungal; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase