title: Synthetic oligonucleotide probes complementary to the HLA-B variable region hybridize with Klebsiella pneumoniae.
abstract: Three oligonucleotide probes complementary to base sequences of the HLA-B variable region were used to probe clinical and foodborne isolates of Klebsiella spp. by DNA colony hybridization. One oligonucleotide (RR-3) corresponding to amino acid residues 66-74 of the HLA-B27.1 sequence and one corresponding to residues 66-74 of the HLA-B7 sequence (RR-5) hybridized with K. pneumoniae under conditions of high stringency. A genomic library was constructed using K. pneumoniae K43 chromosomal DNA, and a 1 kb PstI restriction fragment was found to contain the sequence associated with specific binding of the oligonucleotide probes. After purification and radiolabeling, the cloned fragment hybridized with 96.2% of the K. pneumoniae isolates by DNA colony hybridization. These results confirm the presence of sequence similarities between bacterial DNA and the MHC Class I variable region.
authors.affiliation: Division of Microbiology, Food and Drug Administration, Washington, DC 20204.
authors.forename: K M
authors.initials: KM
authors.lastname: Williams
authors.forename: T
authors.initials: T
authors.lastname: Hirofuji
authors.forename: R B
authors.initials: RB
authors.lastname: Raybourne
authors.forename: D L
authors.initials: DL
authors.lastname: Shook
authors.forename: M W
authors.initials: MW
authors.lastname: Trucksess
authors.forename: D T
authors.initials: DT
authors.lastname: Yu
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D015235:HLA-B Antigens / Q000235:genetics*; D006801:Humans; D007711:Klebsiella pneumoniae / Q000235:genetics* / Q000276:immunology; D015345:Oligonucleotide Probes / Q000138:chemical synthesis / Q000235:genetics*; D010957:Plasmids; D012689:Sequence Homology, Nucleic Acid; D013167:Spondylitis, Ankylosing / Q000382:microbiology
chemical_list: D000942:Antigens, Bacterial; D004269:DNA, Bacterial; D015235:HLA-B Antigens; D015345:Oligonucleotide Probes