Yeast mutants conferring resistance to toxic effects of cloned human insulin-like growth factor I.
The production of extracellular human insulin-like growth factor I (IGF-I) in yeast is deleterious to the growth of the host organism. Mutants resistant to the toxic effects of IGF-I production were isolated. A subset of these mutants produced levels of IGF-I greater than the parent strain and were due to chromosomal recessive mutations at a single locus, hpx1. The overproduction of IGF-I was independent of the original promoter and vector expression system. The mutant strains also displayed enhanced extracellular production of other heterologous proteins.
Shuster
J R
JR
Chiron Corporation, Emeryville, CA 94608.
Moyer
D L
DL
Lee
H
H
Dennis
A
A
Smith
B
B
Merryweather
J P
JP
D002268:Carboxypeptidases / Q000032:analysis; D004352:Drug Resistance, Microbial / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000378:metabolism / Q000633:toxicity*; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D013002:Somatomedins / Q000633:toxicity*; D014170:Transformation, Genetic
D013002:Somatomedins; D007334:Insulin-Like Growth Factor I; D002268:Carboxypeptidases; C022471:proteinase C