Elevation of c-myc protein by DNA strand breakage.
The intracellular level of the c-myc protein is elevated in a dose and time dependent manner by DNA strand breakage. Lesions introduced by either alkylating agents or gamma irradiation are capable of stimulating increased production of c-myc protein. In addition, inhibition of DNA strand break rejoining maintains the level of the c-myc protein in an elevated state, whilst inhibition of DNA replication does not cause an increase in c-myc protein. We conclude that chromatin perturbation via DNA strand breakage both increases the endogenous amounts of the c-myc protein and maintains its elevated level.
Sullivan
N F
NF
Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, UK.
Willis
A E
AE
D000477:Alkylating Agents / Q000494:pharmacology; D002460:Cell Line; D004249:DNA Damage; D004261:DNA Replication / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007700:Kinetics; D011505:Protein-Tyrosine Kinases / Q000096:biosynthesis*; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000096:biosynthesis*; D016271:Proto-Oncogene Proteins c-myc; D011519:Proto-Oncogenes
D000477:Alkylating Agents; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D011505:Protein-Tyrosine Kinases