title: Proteinic prorenin-releasing-stimulator (PRS) in the rat submandibular gland.
abstract: The release of prorenin as well as renin from rat renal slices was confirmed by a rat prorenin-prosegment ELISA system and an assay system for determining the renin activity. A significant increase of the prorenin release was found by adding rat submandibular gland extract to the slice medium, indicating the existence of a prorenin-releasing stimulator (PRS) in the extract. The pI and molecular mass of PRS were 8.5-8.7 and 28-30 kDa, respectively. The PRS was completely inactivated by boiling or a proteinase treatment.
authors.affiliation: Department of Biotechnology, Faculty of Agriculture, Gifu University, Japan.
authors.forename: F
authors.initials: F
authors.lastname: Suzuki
authors.forename: S
authors.initials: S
authors.lastname: Miyazaki
authors.forename: M
authors.initials: M
authors.lastname: Uozumi
authors.forename: N
authors.initials: N
authors.lastname: Okamoto
authors.forename: S
authors.initials: S
authors.lastname: Yamashita
authors.forename: K
authors.initials: K
authors.lastname: Murakami
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D003470:Culture Media; D010450:Endopeptidases; D004792:Enzyme Precursors / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D012083:Renin / Q000378:metabolism*; D013363:Submandibular Gland / Q000378:metabolism*; D014020:Tissue Extracts / Q000494:pharmacology*; D014357:Trypsin
chemical_list: D003470:Culture Media; D004792:Enzyme Precursors; D014020:Tissue Extracts; D010450:Endopeptidases; D014357:Trypsin; D012083:Renin