title: Contribution of partial pancreatectomy, systemic hormone delivery, and duct obliteration to glucose regulation in canine pancreas. Importance in pancreas transplantation.
abstract: Our aim was to isolate and determine the contribution of partial pancreatectomy, systemic delivery of pancreatic hormones, and duct obliteration to glucose regulation after segmental pancreas transplantation in dogs. Fasting, postprandial, and intravenous glucose-stimulated glucose, insulin, glucagon, pancreatic polypeptide (PP), and cholecystokinin (CCK) and intravenous bombesin-stimulated PP levels were studied in beagles at three successive intervals in a crossover design. The first was 6 wk after partial (approximately 70%) pancreatectomy with intact regular enteric exocrine drainage from the duodenal pancreatic remnant, the next was 2 wk after venous transposition with systemic delivery of pancreatic hormones, and the third was 6 wk after in situ duct obliteration of the remnant. With partial pancreatectomy, K values were modestly diminished (30%), and a concomitant reduction of second-phase intravenous glucose-stimulated insulin release was observed. Other parameters were not significantly affected. Venous transposition doubled peripheral plasma levels of insulin under all conditions. Fasting glucose, PP, and CCK levels decreased slightly. Other parameters were not affected. Duct obliteration of the systemic draining pancreatic remnants seriously impaired glucose sensitivity, resulting in a 50% reduction of K values and fasting and sustained postprandial hyperglycemia (approximately 8 mM) and a 70-50% reduction (acute and overall responses, respectively) of intravenous glucose-stimulated insulin. Fasting hormone and postprandial insulin, glucagon, and CCK levels were not affected. The postprandial PP response was severely reduced, and bombesin-stimulated PP release was abolished by duct obliteration. We conclude that histological changes associated with duct obliteration are the major determinants of glucose regulation in segmental pancreas transplantation.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Surgery, University Hospital Leiden, The Netherlands.
authors.forename: M P
authors.initials: MP
authors.lastname: van der Burg
authors.forename: H G
authors.initials: HG
authors.lastname: Gooszen
authors.forename: O R
authors.initials: OR
authors.lastname: Guicherit
authors.forename: J B
authors.initials: JB
authors.lastname: Jansen
authors.forename: M
authors.initials: M
authors.lastname: Frölich
authors.forename: F A
authors.initials: FA
authors.lastname: van Haastert
authors.forename: C B
authors.initials: CB
authors.lastname: Lamers
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D001839:Bombesin / Q000494:pharmacology; D002766:Cholecystokinin / Q000097:blood; D004285:Dogs; D005215:Fasting; D005934:Glucagon / Q000097:blood; D005947:Glucose / Q000008:administration & dosage; D007328:Insulin / Q000097:blood; D009387:Neoprene / Q000008:administration & dosage; D010179:Pancreas / Q000187:drug effects / Q000502:physiology*; D016035:Pancreas Transplantation; D010180:Pancreatectomy / Q000379:methods*; D010183:Pancreatic Ducts / Q000187:drug effects*; D010187:Pancreatic Hormones / Q000097:blood*; D010191:Pancreatic Polypeptide / Q000097:blood; D013997:Time Factors
chemical_list: D001786:Blood Glucose; D007328:Insulin; D010187:Pancreatic Hormones; D010191:Pancreatic Polypeptide; D005934:Glucagon; D009387:Neoprene; D002766:Cholecystokinin; D005947:Glucose; D001839:Bombesin