New crystal form of recombinant murine interferon-beta.
Although we have reported (Matsuda, S., Kawano, G., Itoh, S., Mitsui, Y., and Iitaka, Y. (1986) J. Biol. Chem. 261, 16207-16209) that recombinant murine interferon-beta produced in Escherichia coli was crystallized in an orthorhombic space group C222(1) using polyethyleneglycol 8000 as precipitant, the crystals had an insufficient resolution and a marked tendency for orientational disorder around the c axis. We now report that another form of murine interferon-beta crystals with little disorder was obtained in the presence of dioxane using ammonium sulfate as precipitant. The new crystals belong to a hexagonal space group P6(1) or P6(5) with a = b = 71.4 A and c = 79.6 A having only one murine interferon-beta molecule in an asymmetric unit. The crystals are reasonably stable to x-rays and significantly diffract up to 2.2 A resolution when a synchrotron beam is used.
Matsuda
S
S
Basic Research Laboratories, Toray Industries, Inc., Kamakura, Japan.
Senda
T
T
Itoh
S
S
Kawano
G
G
Mizuno
H
H
Mitsui
Y
Y
D000818:Animals; D003460:Crystallization; D004926:Escherichia coli / Q000235:genetics; D007370:Interferon Type I / Q000302:isolation & purification; D051379:Mice; D011994:Recombinant Proteins; D014961:X-Ray Diffraction
D007370:Interferon Type I; D011994:Recombinant Proteins