[The growth and morphophysiological study of the bushy tissue receptor in a nerve tissue culture].
The conditions for growing bush receptors on collagen substrate in presence of 2-4 ng/ml highly purified brain neurite stimulating protein were determined in the study of spinal ganglia cultures of 10-11-day chicken embryos. The degree of neurite branching and sinuosity and the distribution density of preterminals of cultured bush-like receptors are like those of chicken embryos' natural interoceptors. The ultrastructure of end receptor plates in culture resembles that of natural mechanoreceptor terminals. Cultural receptors are characterized by spontaneous electrophysiological activity. They respond to tactile irritation with bursts of discharges.
Sotnikov
O S
OS
Chalisova
N I
NI
Maĭorov
V N
VN
Akoev
G N
GN
Goncharova
V P
VP
D000818:Animals; D002642:Chick Embryo; D003094:Collagen; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods; D005727:Ganglia, Spinal / Q000502:physiology / Q000648:ultrastructure; D008465:Mechanoreceptors / Q000254:growth & development* / Q000648:ultrastructure; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast
D003470:Culture Media; D003094:Collagen
Vyrashchivanie i morfo-fiziologicheskoe issledovanie kustikovidnogo tkanevogo retseptora v kul'ture nervnoĭ tkani.