A sensitive method for the determination of the mitochondrial susceptibility factor (MSF).
A sensitive method for the determination of the activity of MSF in the cytosol of reticulocytes is described. It is based on the fact that proteolysis products from the mitochondria containing stroma of immature reticulocytes (fraction I) are only liberated if MSF is added. The reproducibility of the method is documented with partly purified MSF. A drawback is the limited range of MSF which can be tested.
Schmidt
J
J
Institute of Biochemistry, Humboldt University Berlin, GDR.
Rapoport
S M
SM
D000818:Animals; D003600:Cytosol / Q000032:analysis; D008928:Mitochondria / Q000032:analysis* / Q000378:metabolism; D010447:Peptide Hydrolases / Q000097:blood; D012156:Reticulocytes / Q000032:analysis*
D010447:Peptide Hydrolases