Effects of contrast media on endothelial cell monolayers under controlled flow conditions.
To compare the biocompatibility of nonionic and ionic intravascular contrast media in a more physiologic in vitro system, key aspects of blood flow through a microvessel were simulated. Toward this end, monolayers of endothelial cells placed in a specially designed flow chamber, real-time imaging of the monolayers by brightfield and phase videomicroscopy, and real-time imaging and computer-aided quantitation at normal hematocrit levels of platelet adhesion/aggregation to sites of monolayer injury by means of epifluorescence videomicroscopy were used. At a concentration in culture medium of 20% by volume, it was found that monolayer morphology was least altered by iohexol when compared with diatrizoate and ioxaglate; monolayer production of prostacyclin was enhanced (p less than 0.001) by ioxaglate compared with saline controls; and at a concentration in citrated blood of 20% by non-red-cell volume, platelet adhesion/aggregation was reduced by all 3 contrast agents in the order diatrizoate greater than ioxaglate greater than iohexol.
Grabowski
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Division of Pediatric Hematology/Oncology, New York Hospital-Cornell University Medical Center, New York 10021.
D000328:Adult; D000818:Animals; D001011:Aorta / Q000187:drug effects; D002417:Cattle; D002478:Cells, Cultured; D003287:Contrast Media / Q000494:pharmacology*; D003973:Diatrizoate / Q000494:pharmacology; D004727:Endothelium / Q000187:drug effects; D011464:Epoprostenol / Q000096:biosynthesis; D006801:Humans; D007472:Iohexol / Q000494:pharmacology; D007485:Ioxaglic Acid / Q000494:pharmacology; D008833:Microcirculation / Q000187:drug effects; D010973:Platelet Adhesiveness / Q000187:drug effects*; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors
D003287:Contrast Media; D010975:Platelet Aggregation Inhibitors; D003973:Diatrizoate; D007472:Iohexol; D011464:Epoprostenol; D007485:Ioxaglic Acid