Cloning of mercury-resistance gene from R-plasmid in Escherichia coli isolated from dental hospital sewage.
1. Mercury-resistance gene was cloned from a drug-resistance plasmid in Escherichia coli isolated from dental hospital sewage. 2. A 7.7 kb mercury-resistance gene was successfully cloned and expressed in pJC74 cosmid and pBR 322 plasmid vectors. 3. High activities of mercury reductase and 203Hg vaporization were demonstrated in cell extracts of mercury-resistant clone. 4. Mercury reductase activity was localized in the cytoplasm. 5. By colony DNA-DNA hybridization, 32P-labelled 7.7-kb DNA hybridized with mercury-resistant E. coli isolates from dental hospital sewage.
Arii
H
H
Department of Biochemistry, Nihon University School of Dentistry, Chiba, Japan.
Abiko
Y
Y
D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000302:isolation & purification; D003764:Dental Materials / Q000032:analysis* / Q000633:toxicity; D004351:Drug Resistance; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D008628:Mercury / Q000032:analysis / Q000633:toxicity*; D008631:Mercury Radioisotopes; D010088:Oxidoreductases / Q000235:genetics*; D010957:Plasmids; D012722:Sewage / Q000032:analysis*; D014170:Transformation, Genetic; D014866:Waste Products / Q000032:analysis*; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity
D004269:DNA, Bacterial; D003764:Dental Materials; D008631:Mercury Radioisotopes; D012722:Sewage; D014866:Waste Products; D014874:Water Pollutants, Chemical; D010088:Oxidoreductases; C021551:mercuric reductase; D008628:Mercury