title: Preparative purification of Plasmodium falciparum circumsporozoite protein synthetic polypeptides by displacement chromatography.
abstract: Displacement chromatography was used for the preparative purification of a synthetic polypeptide that is a promising malaria vaccine. It was prepared by solid-phase synthesis and contains two important epitopes of circumsporozoite (CS) protein of Plasmodium falciparum sporozoite. With apparatus typically employed in analytical high-performance liquid chromatography (HPLC) and on a 250 x 4.6 mm I.D. reversed-phase column, up to 50 mg of crude polypeptide were purified in a single run and with a yield higher than 95%. The results demonstrate that displacement chromatography is suitable for the isolation of several milligrams of a pure polypeptide from a complex mixture that is difficult to separate even by analytical HPLC. In such a preparative application, displacement appears to be superior to elution chromatography as used traditionally.
authors.affiliation: Laboratory of Peptide Synthesis, SCLAVO, Rome, Italy.
authors.forename: G C
authors.initials: GC
authors.lastname: Viscomi
authors.forename: A
authors.initials: A
authors.lastname: Ziggiotti
authors.forename: A S
authors.initials: AS
authors.lastname: Verdini
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D008969:Molecular Sequence Data; D010455:Peptides / Q000302:isolation & purification*; D010963:Plasmodium falciparum / Q000276:immunology*; D015800:Protozoan Proteins
chemical_list: D000953:Antigens, Protozoan; D010455:Peptides; D015800:Protozoan Proteins; C041595:circumsporozoite protein, Protozoan