[Morphological changes of pancreatic B cells in ventromedial hypothalamic (VMH)-lesioned obese rats].
Morphological changes of pancreatic B cells were investigated in ventromedial hypothalamic (VMH)-lesioned obese rats. An increase in body weight, a decrease in body length, and a marked increase in fat-pad were observed in VMH-lesioned obese rats. The volume density of pancreatic islets and that of B cells in VMH-lesioned obese rats were more increased than those in sham-operated control rats. The B cells of VMH-lesioned obese rats often showed a slight reaction for aldehyde fuchsin or anti-pig insulin serum. By the electron microscopy, the degranulated B cells were found to contain well developed Golgi apparatus and rough endoplasmic reticulum. These observations indicate increased activities of synthesis and release of insulin. We concluded that VMH-lesioned obesity was caused by accelerated lipogenesis with hypersecretion of insulin.
Miyamoto
Y
Y
Department of Animal Science, Faculty of Agriculture, Kyoto University, Japan.
Miyamoto
H
H
Sato
E
E
D000818:Animals; D005260:Female; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000473:pathology* / Q000648:ultrastructure; D008055:Lipids / Q000096:biosynthesis; D009765:Obesity / Q000209:etiology / Q000473:pathology*; D051381:Rats; D011919:Rats, Inbred Strains; D014697:Ventromedial Hypothalamic Nucleus / Q000502:physiology*
D007328:Insulin; D008055:Lipids