Asparaginyl-tRNA synthetase from Escherichia coli has significant sequence homologies with yeast aspartyl-tRNA synthetase.
The Escherichia coli asnS gene codes for asparaginyl-tRNA synthetase (NRSEC). We have sequenced the asnS region, including 382 bp of the 5'-untranslated region, 1398 bp of the coding region and 280 bp of the 3'-untranslated region. The DNA-derived NRSEC amino acid (aa) sequence was confirmed by direct aa sequencing of the N-terminal parts of the native protein and of a 28-kDa internal fragment generated by trypsin digestion. The asnS gene product has been purified to homogeneity using three chromatographic steps. Sequence comparison of the deduced NRSEC sequence with all aminoacyl-tRNA synthetase sequences showed significant homologies with the yeast aspartyl-tRNA synthetase and weaker relationships with other aminoacyl-tRNA synthetases for aa with an XAX codon.
Anselme
J
J
European Molecular Biology Laboratory, Grenoble, France.
Härtlein
M
M
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics*; D001226:Aspartate-tRNA Ligase / Q000235:genetics*; D001407:Bacillus / Q000201:enzymology / Q000235:genetics; D001483:Base Sequence; D003062:Codon; D004269:DNA, Bacterial; D004271:DNA, Fungal; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D012346:RNA, Transfer, Amino Acyl; D012689:Sequence Homology, Nucleic Acid; D015003:Yeasts / Q000201:enzymology / Q000235:genetics
D000596:Amino Acids; D003062:Codon; D004269:DNA, Bacterial; D004271:DNA, Fungal; D012346:RNA, Transfer, Amino Acyl; D000604:Amino Acyl-tRNA Synthetases; D001226:Aspartate-tRNA Ligase; C029194:asparaginyl-tRNA synthetase