Human immunodeficiency virus type 1 rev protein as a negative trans-regulator.
Even though the rev gene of the human immunodeficiency virus type 1 (HIV-1) is essential for viral replication, high levels of rev also downregulate viral gene expression. As the degree of rev protein expression exceeds expression of wild-type virus, a gradient of decreasing viral mRNA synthesis becomes evident. The target sequence for this downregulation resides outside of trans-activating region (TAR) and upstream from the enhancer sequences in the long terminal repeat (LTR), suggesting that regulation is at a transcriptional level.
Sadaie
M R
MR
Laboratory of Tumor Cell Biology, National Cancer Institute, Bethesda, MD 20892.
Benaissa
Z N
ZN
Cullen
B R
BR
Wong-Staal
F
F
D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D015536:Down-Regulation; D015967:Gene Expression Regulation, Viral; D015694:Gene Products, rev / Q000096:biosynthesis / Q000235:genetics*; D005816:Genetic Complementation Test; D015497:HIV-1 / Q000235:genetics*; D010957:Plasmids; D012333:RNA, Messenger / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis; D012045:Regulatory Sequences, Nucleic Acid; D012091:Repetitive Sequences, Nucleic Acid / Q000502:physiology; D015534:Trans-Activators / Q000235:genetics*; D015533:Transcriptional Activation; D014162:Transfection; D014779:Virus Replication; D054321:rev Gene Products, Human Immunodeficiency Virus
D015694:Gene Products, rev; D012333:RNA, Messenger; D012367:RNA, Viral; D015534:Trans-Activators; D054321:rev Gene Products, Human Immunodeficiency Virus; D015500:Chloramphenicol O-Acetyltransferase