[Studies on pepsinogen production in advanced gastric cancer tissue].
This study was carried out to elucidate a role of pepsinogen, one of the proteases that currently introduced as a promotor of tumor invasion, on gastric cancer spread. Production of pepsinogen in cancer tissue was analysed by means of three different methods of acid proteolytic activity (APA), polyacrylamide gel electrophoresis (PAGE), and immunoperoxidase staining (IPS), respectively in the 60 resected advanced gastric cancer specimens. Mean level of APA in cancer tissue was higher than that in the control intact muscle layer of the gastric body (p less than 0.05). Through IPS and PAGE, pepsinogen was detected in 53 and 78% of cancer tissue, respectively. Cancer tissue in the cardia produced higher pepsinogen than that in the pyrolus (p less than 0.05). Cancer tissue of Borrmann IV type illustrated higher level of pepsinogen than those of Borrmann II or III type (p less than 0.05). Furthermore, cancer tissue with advanced serosa invasion or that belonging Stage IV presented relatively higher levels. There was a significant correlation between nodal involvement and pepsinogen level (p less than 0.05). Thus, pepsinogen in the gastric cancer tissue tended to be detected with cancer spread. Pepsinogen produced in cancer tissue could loosen or separate intercellular connection of cancer cells to form isolated signet ring cells.
Saito
T
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Department of Surgery, Sendai National Hospital, Japan.
D000328:Adult; D000367:Age Factors; D000368:Aged; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D010435:Pepsinogens / Q000032:analysis / Q000096:biosynthesis*; D012737:Sex Factors; D013274:Stomach Neoplasms / Q000201:enzymology*
D010435:Pepsinogens