Mutational analysis of the interactions between U1 small nuclear RNA and pre-mRNA of yeast.
In recent experiments we have used the power of yeast genetics to study U1 small nuclear RNA (snRNA): pre-messenger RNA (pre-mRNA) base pairing interactions [Séraphin et al. EMBO J. 7 (1988) 2533-2538]. Here we extend these observations to other potential U1 snRNA: pre-mRNA pairings. We show that several U1 snRNA mutants are viable. Using these U1 mutant strains we demonstrate further a base-pairing interaction between U1 snRNA position 3 and intron position 6. However, this interaction is only detected with a poor splicing substrate containing branchpoint mutations. These results provide information on the mechanism of 5' splice site-branch point interaction. We also propose several models which may explain why the sequence of the 5' end of the U1 snRNA is conserved among organisms as divergent as man and yeast.
Séraphin
B
B
Department of Biology, Brandeis University, Waltham, MA 02254.
Rosbash
M
M
D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D007438:Introns; D008322:Mammals / Q000235:genetics; D012322:RNA Precursors / Q000235:genetics*; D012326:RNA Splicing; D012331:RNA, Fungal / Q000235:genetics*; D012342:RNA, Small Nuclear / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D012322:RNA Precursors; D012331:RNA, Fungal; D012342:RNA, Small Nuclear