title: Bovine olfactory cilia preparation: thiol-modulated odorant-sensitive adenylyl cyclase.
abstract: We have characterized the adenylyl cyclase activity in a newly developed preparation of isolated olfactory cilia from the bovine chemosensory neuroepithelium. Like its counterparts from frog and rat, the ciliary enzyme was stimulated by guanine nucleotides, by forskolin, and by a variety of odorants in the presence of GTP. The main difference between the bovine olfactory cilia preparation and the frog and rat olfactory cilia preparation is that odorant stimulation of the bovine olfactory adenylyl cyclase is strongly inhibited by submillimolar concentrations of dithiothreitol. This inhibition is a consequence of a concomitant increase in the GTP-stimulated level and the decrease of the odorant stimulation of the enzyme. Nasal respiratory cilia have a much lower level of adenylyl cyclase activity and show no odorant stimulation. Owing to the large quantities of material available, the bovine olfactory cilia preparation is advantageous for studies of the proteins involved in chemosensory transduction.
authors.affiliation: Department of Membrane Research, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: D
authors.initials: D
authors.lastname: Lazard
authors.forename: Y
authors.initials: Y
authors.lastname: Barak
authors.forename: D
authors.initials: D
authors.lastname: Lancet
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000201:enzymology; D002469:Cell Separation; D002923:Cilia / Q000201:enzymology*; D005576:Colforsin / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology*; D004789:Enzyme Activation; D004848:Epithelium / Q000201:enzymology; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate / Q000494:pharmacology; D009812:Odorants; D009831:Olfactory Mucosa / Q000201:enzymology*; D051381:Rats; D015290:Second Messenger Systems; D012903:Smell; D013873:Thionucleotides / Q000494:pharmacology; D014420:Turbinates / Q000201:enzymology*
chemical_list: D013873:Thionucleotides; D005576:Colforsin; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate; D000262:Adenylyl Cyclases; D004229:Dithiothreitol