Distribution of laminin, collagen type IV, collagen type I, and fibronectin in chicken cardiac jelly/basement membrane.
Light microscopic immunolabeling studies were designed to identify and locate structural components within the cell-free extracellular matrix which lies between the embryonic endocardial and myocardial tubes. Affinity-purified antibodies were used to examine stage 15-22 embryonic chicken hearts. Specimens were immunolabeled by using three different methodologies: 1) postembedding labeling of 10 microns cryostat sections, 2) preembedding labeling (en bloc) of whole hearts, and 3) postembedding labeling of ethanol/acetic acid-fixed paraffin sections. Our results establish the spatial distribution of collagen type I and demonstrate for the first time the presence of collagen type IV and laminin in the myocardial-basement-membrane/cardiac jelly.
Little
C D
CD
Department of Anatomy and Cell Biology, School of Medicine, University of Virginia, Charlottesville 22908.
Piquet
D M
DM
Davis
L A
LA
Walters
L
L
Drake
C J
CJ
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D001485:Basement Membrane / Q000378:metabolism; D002642:Chick Embryo; D003094:Collagen / Q000276:immunology / Q000378:metabolism*; D005109:Extracellular Matrix / Q000378:metabolism / Q000648:ultrastructure; D005353:Fibronectins / Q000276:immunology / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D007797:Laminin / Q000276:immunology / Q000378:metabolism*; D009206:Myocardium / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure
D000906:Antibodies; D005353:Fibronectins; D007797:Laminin; D003094:Collagen