The v-myb oncogene product binds to and activates the promyelocyte-specific mim-1 gene.
The v-myb oncogene induces myeloid leukemias in chickens, transforms myeloid cells in vitro, and encodes a sequence-specific DNA binding protein. We used differential hybridization to screen for v-myb-regulated genes in cells transformed by a temperature-sensitive mutant of the oncogene and identified a new gene, mim-1, which encodes a specifically expressed, secretable protein contained in the granules of both normal and v-myb-transformed promyelocytes. The promoter of the mim-1 gene contains three closely spaced binding sites for v-myb protein and is strongly activated by v-myb in a cotransfection assay. Synthetic copies of the binding sites are both necessary and sufficient to confer v-myb protein-dependent activation to a heterologous promoter. We conclude that mim-1 is a cellular gene that is directly regulated by the product of the v-myb oncogene.
Ness
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SA
Differentiation Programme, European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
Marknell
A
A
Graf
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T
D000595:Amino Acid Sequence; D000818:Animals; D009189:Avian Myeloblastosis Virus / Q000201:enzymology / Q000235:genetics; D001483:Base Sequence; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D002645:Chickens; D003001:Cloning, Molecular; D003850:Deoxyribonuclease I; D004926:Escherichia coli / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D005809:Genes, Regulator; D008969:Molecular Sequence Data; D020626:Oncogene Proteins v-myb; D009857:Oncogenes; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation
D020626:Oncogene Proteins v-myb; D012333:RNA, Messenger; D015682:Retroviridae Proteins, Oncogenic; D011505:Protein-Tyrosine Kinases; D003850:Deoxyribonuclease I