Endothelial interleukin-8: a novel inhibitor of leukocyte-endothelial interactions.
Certain inflammatory stimuli render cultured human vascular endothelial cells hyperadhesive for neutrophils. This state is transient and reversible, in part because activated endothelial cells secrete a leukocyte adhesion inhibitor (LAI). LAI was identified as endothelial interleukin-8 (IL-8), the predominant species of which is an extended amino-terminal IL-8 variant. At nanomolar concentrations, purified endothelial IL-8 and recombinant human IL-8 inhibit neutrophil adhesion to cytokine-activated endothelial monolayers and protect these monolayers from neutrophil-mediated damage. These findings suggest that endothelial-derived IL-8 may function to attenuate inflammatory events at the interface between vessel wall and blood.
Gimbrone
M A
MA
Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115.
Obin
M S
MS
Brock
A F
AF
Luis
E A
EA
Hass
P E
PE
Hébert
C A
CA
Yip
Y K
YK
Leung
D W
DW
Lowe
D G
DG
Kohr
W J
WJ
D000595:Amino Acid Sequence; D001685:Biological Factors / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D002630:Chemotactic Factors / Q000302:isolation & purification* / Q000494:pharmacology; D003470:Culture Media / Q000032:analysis; D016207:Cytokines; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D016209:Interleukin-8; D007378:Interleukins / Q000302:isolation & purification* / Q000494:pharmacology; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D011994:Recombinant Proteins / Q000494:pharmacology
D001685:Biological Factors; D002630:Chemotactic Factors; D003470:Culture Media; D016207:Cytokines; D007375:Interleukin-1; D016209:Interleukin-8; D007378:Interleukins; D011994:Recombinant Proteins