[Biosynthesis of the membrane protein of the acquired immunodeficiency syndrome virus (HIV) with a deleted hydrophobic region in E. coli cells].
Effect of the structure of cloned env gene of HIV virus on the level of its expression has been studied. The deletion of the hydrophobic region from the env gene has been shown to increase considerably the biosynthesis of antigen-specific proteins in Escherichia coli cells. At the same time, the low level of expression of the gene fragment the transcription of which is controlled by a powerful lac-promoter suggests the presence of toxic regions of nonhydrophobic nature in the synthesized antigen.
Starov
A I
AI
Rubtsov
P M
PM
Birkin
Iu A
IuA
Vorob'ev
S M
SM
Vengerov
Iu Iu
IuIu
Skriabin
K G
KG
D004279:DNA, Viral / Q000302:isolation & purification; D004926:Escherichia coli / Q000378:metabolism*; D015967:Gene Expression Regulation, Viral; D006678:HIV / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids; D014170:Transformation, Genetic; D014759:Viral Envelope Proteins / Q000096:biosynthesis* / Q000235:genetics
D004279:DNA, Viral; D014759:Viral Envelope Proteins
Biosintez obolochechnogo elka virusa sindroma priobretennogo immunodefitsita (HIV) s udalennoĭ gidrofobnoĭ oblast'iu v kletkakh E. coli.