Cloning and sequence analysis of the gene encoding invertase from the yeast Schwanniomyces occidentalis.
The gene encoding invertase (INV) has been cloned from Schwanniomyces occidentalis. The enzyme consists of 533 amino acids, 8 potential glycosylation sites and has a 45% identity with the invertase from Saccharomyces cerevisiae. The proenzyme has a 22 amino acid signal sequence that has a high alpha-helical transmembrane potential which differs significantly from that predicted for the Saccharomyces cerevisiae enzyme.
Klein
R D
RD
Molecular Biology Research, Upjohn Company, Kalamazoo, MI 49007.
Poorman
R A
RA
Favreau
M A
MA
Shea
M H
MH
Hatzenbuhler
N T
NT
Nulf
S C
SC
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon / Q000502:physiology; D004271:DNA, Fungal / Q000235:genetics; D006026:Glycoside Hydrolases / Q000235:genetics*; D008969:Molecular Sequence Data; D012045:Regulatory Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D004718:Saccharomycetales / Q000201:enzymology / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D043324:beta-Fructofuranosidase
D003062:Codon; D004271:DNA, Fungal; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase