Use of a novel air separation system in a fed-batch fermentative culture of Escherichia coli.
A novel air separation system based on permeable membrane gas separation technology was used to cultivate Escherichia coli. The system fulfilled the dissolved oxygen requirements of a culture of E. coli grown on a glucose synthetic medium at a high and constant growth rate of 0.55 h-1. A biomass yield of 45 g (dry weight) per liter was achieved, and no by-product inhibition by acetate or CO2 was observed.
Fass
R
R
Biotechnology Unit, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892.
Clem
T R
TR
Shiloach
J
J
D003470:Culture Media; D004926:Escherichia coli / Q000254:growth & development* / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D010100:Oxygen / Q000378:metabolism*
D003470:Culture Media; D005947:Glucose; D010100:Oxygen