Comparison of buffalo green monkey cells and McCoy cells for isolation of Chlamydia trachomatis in a microtiter system.
A total of 1,229 urogenital samples of patients with sexually transmitted diseases and prostitutes were inoculated simultaneously in McCoy cells and Buffalo green monkey (BGM) cells by using a microtitration technique. BGM cells proved to be slightly more sensitive than McCoy cells, and they usually showed more and larger inclusions and were more resistant to cytotoxic substances in samples. The decrease in sensitivity as a result of mycoplasmal contamination was much more prominent in McCoy cells than in BGM cells.
Krech
T
T
Institute for Medical Microbiology and Virology, University of Düsseldorf, Federal Republic of Germany.
Bleckmann
M
M
Paatz
R
R
D000818:Animals; D001431:Bacteriological Techniques; D002460:Cell Line; D002690:Chlamydia Infections / Q000175:diagnosis; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D008297:Male; D014526:Urethritis / Q000175:diagnosis; D002575:Uterine Cervicitis / Q000175:diagnosis