Characteristic nucleolar-reticular immunofluorescence staining pattern on touch prints of hamster liver: a marker for Scl-70 antibody.
Sera from 48 patients with systemic sclerosis (SSc) and from 62 patients with other connective tissue diseases were studied for nuclear staining patterns by indirect immunofluorescence (IIF) using touch prints of hamster liver, HEp-2 and cryostat sections of monkey esophagus as substrates. Immunodiffusion studies performed in all cases disclosed Scl-70 antibody in 36 SSc patients. The IIF pattern of Scl-70 antibody is characterized by diffuse granular and nucleolar staining on HEp-2, a combined nucleolar and diffuse reticular staining on imprints of hamster liver and large speckled (nucleolar) fluorescence on cryostat sections of monkey esophagus. Touch prints of hamster liver revealed a partially nucleolar fluorescence as a dominant sign of the combined staining. Hamster liver substrate which is easily available might replace the expensive HEp-2 cells for detecting Scl-70 antibody.
Kühn
G
G
Department of Dermatology, Warsaw Medical Academy, Poland.
Jarzabek-Chorzelska
M
M
Kolacinska-Strasz
Z
Z
Blaszczyk
M
M
Chorzelski
T P
TP
Jablonska
S
S
D000818:Animals; D001323:Autoantibodies / Q000032:analysis*; D001324:Autoantigens / Q000276:immunology*; D002466:Cell Nucleolus / Q000276:immunology; D002467:Cell Nucleus / Q000276:immunology; D003240:Connective Tissue Diseases / Q000276:immunology; D006224:Cricetinae; D004264:DNA Topoisomerases, Type I; D004947:Esophagus / Q000276:immunology; D005455:Fluorescent Antibody Technique; D008099:Liver / Q000276:immunology; D009687:Nuclear Proteins / Q000276:immunology*; D012595:Scleroderma, Systemic / Q000276:immunology; D014407:Tumor Cells, Cultured / Q000276:immunology
D001323:Autoantibodies; D001324:Autoantigens; D009687:Nuclear Proteins; C048100:Scl 70 antigen, human; D004264:DNA Topoisomerases, Type I