First crystallization of a phosphoenolpyruvate carboxylase from Escherichia coli.
Two different forms of crystal for a phosphoenolpyruvate carboxylase from Escherichia coli were obtained by the hanging-drop vapor diffusion technique, using polyethylene glycol 4000 as precipitant. The hexagonal crystal in space group P6(2)22 (or P6(4)22) has cell dimensions of a = 131 A and c = 325 A, whereas the orthorhombic crystal in space group I222 has a = 119 A, b = 252 A and c = 83 A. A tetrameric molecule (396,244 Mr), a subunit of which contains 883 amino residues, has a crystallographic 2 symmetry in the hexagonal crystal or 222 symmetry in the orthorhombic crystal, respectively.
Inoue
M
M
Department of Applied Chemistry, Faculty of Engineering, Osaka University, Japan.
Hayashi
M
M
Sugimoto
M
M
Harada
S
S
Kai
Y
Y
Kasai
N
N
Terada
K
K
Izui
K
K
D002262:Carboxy-Lyases; D003460:Crystallization; D004926:Escherichia coli / Q000201:enzymology*; D010730:Phosphoenolpyruvate Carboxylase
D002262:Carboxy-Lyases; D010730:Phosphoenolpyruvate Carboxylase