31P NMR studies of the metabolic status of pig hearts preserved for transplantation.
31P NMR spectroscopy has been used to evaluate the metabolic status of cardioplegically arrested pig hearts. Hearts were stored with Plegisol for up to 12 hours at either 5 degrees C or 12 degrees C. Results indicated that the ATP content of hearts could be maintained (greater than 70% of initial values) for up to 5 hours in the ischemic storage state. The ATP loss was greater at 12 degrees C. PCr was lost exponentially under the same conditions. Functional testing by reperfusing the stored hearts in vitro indicated a good correlation between the ATP content and survivability of the preparations. Twenty-four hour preservation of pig hearts using slow perfusion with a modified cardioplegic solution (Wicomb) allowed for preservation of both PCr and ATP, in all cases, reperfusion of hearts revealed a loss of NMR- visible ATP and PCr.
Forester
G V
GV
Division of Electrical Engineering, National Research Council of Canada, Ottawa.
Saunders
J K
JK
Mainwood
G W
GW
Butler
K W
KW
Scott
J R
JR
Paradis
H
H
Roy
O Z
OZ
Deslauriers
R
R
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D016027:Heart Transplantation; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009206:Myocardium / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism; D010725:Phosphocreatine / Q000378:metabolism; D010758:Phosphorus; D013997:Time Factors; D014021:Tissue Preservation
D010710:Phosphates; D010725:Phosphocreatine; D010758:Phosphorus; D000255:Adenosine Triphosphate