title: A cysteine-histidine-aspartate catalytic triad is involved in glutamine amide transfer function in purF-type glutamine amidotransferases.
abstract: A family of four glutamine amidotransferases has a homologous glutamine amide transfer domain, designated purF-type, that is named after purF-encoded glutamine phosphoribosylpyrophosphate amidotransferase. The glutamine amide transfer domain of approximately 194 amino acid residues is at the NH2 terminus of the protein chain. Site-directed mutagenesis was used to replace several of the 9 invariant amino acids in the glutamine amide transfer domain of glutamine phosphoribosylpyrophosphate amidotransferase. The results indicate that a Cys1-His101-Asp29 catalytic triad is involved in the glutamine amide transfer function of this enzyme. The evidence suggests that His101 functions to increase the nucleophilicity of Cys1, which is used to form a glutamine-enzyme covalent intermediate. Asp29 has a role subsequent to formation of the covalent intermediate. The Cys-His-Asp catalytic triad is implicated in the glutamine amide transfer function of purF-type amidotransferases.
authors.affiliation: Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907.
authors.forename: B
authors.initials: B
authors.lastname: Mei
authors.forename: H
authors.initials: H
authors.lastname: Zalkin
mesh_terms: D000595:Amino Acid Sequence; D000878:Anthranilate Synthase; D001224:Aspartic Acid; D001665:Binding Sites; D003545:Cysteine; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D006639:Histidine; D008969:Molecular Sequence Data; D009154:Mutation; D019884:Nitrogenous Group Transferases; D014166:Transferases / Q000235:genetics* / Q000378:metabolism
chemical_list: D001224:Aspartic Acid; D006639:Histidine; D014166:Transferases; D019884:Nitrogenous Group Transferases; D000878:Anthranilate Synthase; C020655:anthranilate synthase, glutamine amidotransferase subunit; D003545:Cysteine