[Immunohistochemical analysis of the expression of HLA on the trophoblast of normal pregnancy].
The expression of MHC class II antigens (HLA-DR, -DP, -DQ) on trophoblast was analyzed by an immunoperoxidase technique using monoclonal antibodies. Tissues were obtained after pregnancy hysterectomy with the medical indications and after normal deliveries. They were snap-frozen in liquid nitrogen, cut in a cryostat and fixed in acetone for 10 minutes. Mouse monoclonal antibodies used in this study were anti-HLA-ABC (W6/32, Sera lab), anti-HLA-DR (Cappel), anti-HLA-DP (B7/21, Becton Dickinson) and anti-HLA-DQ (anti-Leu-10, Becton Dickinson) in addition to anti-cytokeratin (CAM5.2, Becton Dickinson) to recognize trophoblast. In the first trimester, HLA-DP could be identified on cytotrophoblastic cell column, cell island, extravillous trophoblast invading the decidua basalis, which were positive for HLA-ABC, and villous cytotrophoblast. In term placenta, villous syncytiotrophoblast and extra-villous trophoblast found in the decidua were positive for HLA-DP. In contrast, no trophoblast reacted with anti-HLA-DR or anti-HLA-DQ.
Honma
S
S
Department of Obstetrics and Gynecology, School of Medicine, Niigata University.
Endou
M
M
Uchiyama
M
M
Nakamura
M
M
Kanazawa
K
K
Takeuchi
S
S
D000911:Antibodies, Monoclonal; D002824:Chorionic Villi / Q000276:immunology; D003656:Decidua / Q000276:immunology; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D011247:Pregnancy / Q000276:immunology*; D011261:Pregnancy Trimester, First; D011263:Pregnancy Trimester, Third; D014327:Trophoblasts / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II