title: [Stability to the extreme influences and significance of internal pH of Saccharomyces cerevisiae depending on the density of cellular suspensions].
abstract: The resistance of a Saccharomyces cerevisiae population against gamma-irradiation (400 krd), heating (60 degrees C), repeated freezing--thawing and rehydration of dehydrated cells was shown to depend on the density of a cell suspension. The percentage of damaged cells (stained with primulin) was found to increase in a suspension with decreasing cell concentrations. The effect was also exerted in an oxygen-free medium. The percentage of undamaged cells increased in populations with a lower cell concentration when a more viscous medium (0.2% agar) was used. The intracellular pH dropped down in a denser intact yeast suspension and rose with an increase in the concentration of dehydrated-rehydrated cells.
authors.forename: P B
authors.initials: PB
authors.lastname: Zikmanis
authors.forename: L P
authors.initials: LP
authors.lastname: Auzinia
authors.forename: R V
authors.initials: RV
authors.lastname: Kruche
authors.forename: M V
authors.initials: MV
authors.lastname: Margevicha
authors.forename: S I
authors.initials: SI
authors.lastname: Auzan
authors.forename: M E
authors.initials: ME
authors.lastname: Beker
mesh_terms: D005615:Freezing; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008826:Microbial Sensitivity Tests; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000254:growth & development / Q000528:radiation effects
vernacular_title: Ustoĭchivost' k ékstremal'nym vozdeĭstviiam i znachenie vnutrennego pH Saccharomyces cerevisiae v zavisimost ot plotnosti kletochnykh suspenziĭ.