Planar chromatographic techniques in biomedicine: current status.
In planar chromatography (PLC), the solvent flows through a layer either by means of capillary forces [conventional thin-layer chromatography (TLC)] or by a forced-flow system (over-pressured layer chromatography). Phases and instrumentation currently available are briefly examined. The main applications in biomedicine are reviewed. Although silica gel TLC plates still predominate, interest in other phases is increasing. Unique detection features such as immunostaining are emphasized. Although gas chromatography and high-performance liquid chromatography have superseded TLC in the analysis of carbohydrates, amino acids and indole derivatives, interest in PLC continues to be high in lipid analysis.
Siouffi
A M
AM
Laboratoire de Génie Chimique et Chimie Appliquée, Faculté des Sciences et Techniques de St-Jérôme, Avenue Escadrille Normandie Niémen, Marseille, France.
Mincsovics
E
E
Tyihak
E
E
D002624:Chemistry, Clinical / Q000379:methods*; D002845:Chromatography / Q000379:methods*