title: Yeast mutants conferring resistance to toxic effects of cloned human insulin-like growth factor I.
abstract: The production of extracellular human insulin-like growth factor I (IGF-I) in yeast is deleterious to the growth of the host organism. Mutants resistant to the toxic effects of IGF-I production were isolated. A subset of these mutants produced levels of IGF-I greater than the parent strain and were due to chromosomal recessive mutations at a single locus, hpx1. The overproduction of IGF-I was independent of the original promoter and vector expression system. The mutant strains also displayed enhanced extracellular production of other heterologous proteins.
authors.affiliation: Chiron Corporation, Emeryville, CA 94608.
authors.forename: J R
authors.initials: JR
authors.lastname: Shuster
authors.forename: D L
authors.initials: DL
authors.lastname: Moyer
authors.forename: H
authors.initials: H
authors.lastname: Lee
authors.forename: A
authors.initials: A
authors.lastname: Dennis
authors.forename: B
authors.initials: B
authors.lastname: Smith
authors.forename: J P
authors.initials: JP
authors.lastname: Merryweather
mesh_terms: D002268:Carboxypeptidases / Q000032:analysis; D004352:Drug Resistance, Microbial / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000378:metabolism / Q000633:toxicity*; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D013002:Somatomedins / Q000633:toxicity*; D014170:Transformation, Genetic
chemical_list: D013002:Somatomedins; D007334:Insulin-Like Growth Factor I; D002268:Carboxypeptidases; C022471:proteinase C