Initiation of transcription on nucleosomal templates.
We describe a simple method that uses curved DNAs to move nucleosomes relative to a sequence of interest. With this method, small changes in the association of a T7 RNA polymerase promoter with a nucleosome are shown to lead to significant changes in transcription efficiency.
Wolffe
A P
AP
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892.
Drew
H R
HR
D000818:Animals; D001483:Base Sequence; D002645:Chickens; D003001:Cloning, Molecular; D004247:DNA / Q000138:chemical synthesis / Q000235:genetics*; D003850:Deoxyribonuclease I; D004912:Erythrocytes / Q000378:metabolism; D005821:Genetic Techniques; D006657:Histones / Q000378:metabolism; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000378:metabolism*; D009710:Nucleotide Mapping; D015183:Restriction Mapping; D013698:Templates, Genetic; D014158:Transcription, Genetic
D006657:Histones; D009707:Nucleosomes; D004247:DNA; D003850:Deoxyribonuclease I