title: A problem encountered using staphylococcus/streptococcus supplement.
abstract: A problem was encountered while using a commercial staphylococcus/streptococcus selective supplement in blood agar culture medium. A number of strains of Staphylococcus aureus failed to grow, although they grew well on non-selective media. The phenomenon was shown to be a result of a pH change caused by incubation in a carbon dioxide-enriched atmosphere, potentiating the activity of the nalidixic acid component. It is recommended that media containing this supplement are not incubated in CO2 enriched atmospheres.
authors.forename: C E
authors.initials: CE
authors.lastname: Morton
authors.forename: H A
authors.initials: HA
authors.lastname: Holt
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001431:Bacteriological Techniques; D003470:Culture Media; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development*
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media