Cross-reactivity of murine monoclonal anti-DNA antibodies with human and murine skin: a possible pathogenetic role in skin lesions of lupus erythematosus.
Anti-DNA autoantibodies are known to cross-react with a wide variety of substances including cell-surface molecules. Thus, we examined cross-reactivities of 20 murine monoclonal anti-DNA antibodies with normal human and mouse skin tissues. Hybridomas producing these monoclonal antibodies were established from non-immunized spleen cells from autoimmune MRL-lpr/lpr mice, a strain characterized by spontaneous development of SLE-like disorders including skin changes. They were selected based on their reactivity to DNA in a typical enzyme-linked immunosorbent assay, in which nine monoclonal antibodies were reactive with both double-stranded DNA and single-stranded DNA, whereas nine monoclonals were reactive only with single-stranded DNA. Even though only seven of them were observed to stain nuclei, most of the monoclonal antibodies revealed strong and distinct cross-reactivities to various components of the skin tissues including the epidermal basement membrane, keratinocytes at different locations of the epidermis, melanocytes, Langerhans cells, Thy-1+ dendritic cells in the case of murine skin, and mast cells. Our results suggest a possible role of so-called anti-DNA antibodies with high or low affinities to DNA in the pathogenesis of cutaneous lesions of lupus erythematosus.
Aiba
S
S
Department of Dermatology, Tohoku University School of Medicine, Sendai, Japan.
Yoshie
O
O
Tomita
Y
Y
Tagami
H
H
D000818:Animals; D000974:Antibodies, Antinuclear / Q000276:immunology* / Q000302:isolation & purification; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D003429:Cross Reactions; D003713:Dendritic Cells / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D007801:Langerhans Cells / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000209:etiology; D008407:Mast Cells / Q000276:immunology; D008544:Melanocytes / Q000276:immunology; D051379:Mice; D012867:Skin / Q000276:immunology*
D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal