Direct visualization of an IgM directed against a membrane antigen involved in both cell-matrix interactions and microfilament organization.
Dental mesenchymal cells were cultured in the presence of a monoclonal antibody. MC16A16, consisting of IgM and directed against a hydrophobic 165 kDa protein. Since the epitope recognized by MC16A16 was found to be at the outer cell surface, a direct visualization of IgM antibodies was used to localize the 165 kDa antigen by transmission electron microscopy. The present results demonstrate that the 165 kDa antigen is a membrane protein.
Lesot
H
H
Institut de Biologie Médicale, CNRS LP 6520, Faculté de Médecine 11, Strasbourg, France.
Meyer
J M
JM
Staubli
A
A
Schmitt
D A
DA
Ruch
J V
JV
D008841:Actin Cytoskeleton / Q000648:ultrastructure; D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D007075:Immunoglobulin M; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000032:analysis; D051379:Mice; D008854:Microscopy, Electron; D008970:Molecular Weight; D017830:Octoxynol; D011092:Polyethylene Glycols; D014070:Tooth / Q000166:cytology*
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D007075:Immunoglobulin M; D008565:Membrane Proteins; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40