Escherichia coli K-12 cell-cell interactions seen by time-lapse video.
The high degree of organization in mature bacterial colonies suggests specific interactions between the cells during colony development. We have used time-lapse video microscopy to find evidence for cell-cell interactions. In its initial stages, Escherichia coli K-12 colony morphogenesis displayed control of the geometry of cell growth and involved intimate side-by-side associations. When microcolonies developed from isolated single bacteria, a directed process of elongation and division resulted in the appearance of a symmetrical four-cell array. When growth began with separate but nearby bacteria, the daughters of different cells elongated towards each other and also lined up side by side. Interactions between microcolonies containing several hundred or more bacteria were visible several hours later. Control of cell morphogenesis at later stages of microcolony development was strain specific. These results show that E. coli K-12 cells respond to each other and adjust their cellular morphogenesis to form multicellular groups as they proliferate on agar.
Shapiro
J A
JA
Department of Biochemistry and Molecular Biology, University of Chicago, Illinois 60637.
Hsu
C
C
D002455:Cell Division; D004926:Escherichia coli / Q000166:cytology / Q000254:growth & development / Q000502:physiology*; D009154:Mutation; D013997:Time Factors; D014741:Video Recording / Q000379:methods