New tools for the localization of second messenger systems.
1. Probes available for the localization of components of second messenger systems include G-protein oligonucleotides which have been used to produce cDNA probes to label G-protein mRNA by in situ hybridization histochemistry. 2. Enzymes involved in second messenger responses have been labelled with [3H]-forskolin (Gs-linked adenylate cyclase), [3H]-cAMP (cAMP-dependent protein kinase) and [3H]-PDBu2 (protein kinase C). 3. [3H]-Inositol 1,4,5 trisphosphate labels a site on sarcoplasmic reticulum believed to trigger Ca2+ release. 4. These probes allow the comparison of location of receptors and second messengers and the examination of changes in second messenger systems with drug treatment and disease.
Summers
R J
RJ
Department of Pharmacology, University of Melbourne, Parkville, Victoria, Australia.
McMartin
L R
LR
D000818:Animals; D006801:Humans; D015290:Second Messenger Systems / Q000187:drug effects*