[A new early gene in front of the middle gene 31 of bacteriophage T4: cloning and expression].
We have identified a new gene, which we designated gene 31.1, as the nearest upstream neighbour of gene 31. We cloned the 1.03 k.b. EcoRI/BglII fragment of T4 DNA, and expressed both genes using the T7 RNA polymerase/promoter two plasmid system. Gene 31.1 encodes a protein with molecular weight of about 10 kDa, and the C-terminal portion of this gene is the incomplete open reading frame ORF 31.1 determined earlier. Using primer extension sequencing on RNA templates isolated from T4 mot A+ and mot A- infected cells, we have shown that gene 31 has a middle mode mot A-dependent transcript starting at two points and which is initiated from gene's 31 own promoter. Gene 31 is also transcribed from an early upstream promoter into a polycistronic mRNA which covers an early gene 31.1 as well.
Nivinskas
R G
RG
Raudonikene
A A
AA
Guild
N
N
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004587:Electrophoresis, Agar Gel; D015180:Electrophoresis, Gel, Two-Dimensional; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005814:Genes, Viral; D008969:Molecular Sequence Data; D010957:Plasmids; D012333:RNA, Messenger / Q000032:analysis; D013604:T-Phages / Q000235:genetics*; D014158:Transcription, Genetic
D012333:RNA, Messenger
Novyĭ ranniĭ gen pered srednim genom 31 bakteriofaga T4: klonirovanie i ékspressiia.