title: Biochemical, immunological and physicochemical comparisons between OHIO-1 and four SHV-type beta-lactamases.
abstract: The biochemical, immunological and physicochemical properties of the beta-lactamase OHIO-1 were compared to those of four beta-lactamases commonly found in Klebsiella pneumoniae: SHV-1, SHV-3 and the beta-lactamases of strains GN 11-03 and GN 422. The substrate profile of SHV-1, OHIO-1 and of the beta-lactamases GN 11-03 and GN 422 were similar, while that of SHV-3 appeared comparable to that of the extended spectrum SHV-2. Moreover, anti-TEM-1 serum inactivated OHIO-1 as well as SHV-1 and the beta-lactamases of strains GN 11-03 and GN 422. Analysis of the electrophoretic mobilities, isoelectric points and titration curves demonstrated that OHIO-1 and the 4 other beta-lactamases examined were closely related variants. From these findings it appears that OHIO-1 could be classified among the SHV-type beta-lactamases.
authors.affiliation: UFR Cochin, Laboratoire de Bactériologie, Paris, France.
authors.forename: G
authors.initials: G
authors.lastname: Vedel
authors.forename: G
authors.initials: G
authors.lastname: Paul
authors.forename: B
authors.initials: B
authors.lastname: Picard
authors.forename: A
authors.initials: A
authors.lastname: Philippon
mesh_terms: D004586:Electrophoresis; D004926:Escherichia coli / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007106:Immune Sera; D007525:Isoelectric Focusing; D007711:Klebsiella pneumoniae / Q000201:enzymology*; D010957:Plasmids; D013379:Substrate Specificity; D001618:beta-Lactamases / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
chemical_list: D007106:Immune Sera; D001618:beta-Lactamases