[Study of amino acid utilization by isogenic strains of Escherichia coli in relation to the culture growth and biosynthesis of penicillin amidase].
Dynamics of free amino acid utilization by isogenic strains of Escherichia coli differing in intensity of their growth and levels of penicillin acylase biosynthesis in media containing corn steep liquor or peptone was studied. It was shown that in both the media some amino acids such as serine, threonine, glutaminic and asparaginic acids were actively utilized by the strains mainly during the culture intensive growth while others such as glycine, alanine and tyrosine were actively utilized during the enzyme biosynthesis. Intensively utilized arginine and proline were probably used for the growth and biosynthesis. The other amino acids were not utilized completely from the media. The lowest levels of their utilization were observed when the strains were cultivated in the medium with peptone.
Penzikova
G A
GA
Oreshina
M G
MG
Shishlova
O R
OR
Tsibanov
V V
VV
Ivanina
T A
TA
D000581:Amidohydrolases / Q000096:biosynthesis*; D000596:Amino Acids / Q000378:metabolism*; D003470:Culture Media; D004926:Escherichia coli / Q000502:physiology*; D066298:In Vitro Techniques; D010399:Penicillin Amidase / Q000096:biosynthesis*; D010461:Peptones; D010936:Plant Extracts; D003313:Zea mays
D000596:Amino Acids; D003470:Culture Media; D010461:Peptones; D010936:Plant Extracts; D000581:Amidohydrolases; D010399:Penicillin Amidase
Issledovanie dinamiki potrebleniia aminokislot izogennymi shtammami Escherichia coli v sviazi s rostom kul'tury i biosintezom penitsillinatsilazy.