Trypsin digest of the iris.
We succeeded in obtaining vascular mounts of the iris by modifying the original technique of trypsin digestion described by Kuwabara and Cogan. This was achieved by scraping the pigment epithelium, repeated bleaching, and prolonged enzymatic digestion.
Mansour
A M
AM
Department of Ophthalmology, University of Texas Medical Branch, Galveston.
D003646:Debridement; D005404:Fixatives; D006652:Histological Techniques; D006801:Humans; D007498:Iris / Q000098:blood supply*; D014357:Trypsin
D005404:Fixatives; D014357:Trypsin