DNA photolyase in E. coli: effects on UV mutagenesis by plasmids expressing the phr gene.
Derivatives of an E. coli plasmid pKY33 are described having specific insertions or deletions that effect or do not effect the phr gene (for DNA photolyase) carried in this plasmid. The various plasmids are tested to determine which cause an inhibition of UV mutagenesis producing glutamine tRNA ochre suppressor mutations. The inhibition is found to require a functional phr gene, which substantiates our earlier report that amplified DNA photolyase interferes specifically with a category of mutagenesis involving targeting by a pyrimidine dimer.
Yamamoto
K
K
Pathology Laboratory, Osaka Prefectural Institute of Public Health, Japan.
Bockrath
R
R
D004255:Deoxyribodipyrimidine Photo-Lyase / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology / Q000528:radiation effects*; D005786:Gene Expression Regulation; D008190:Lyases / Q000235:genetics*; D009154:Mutation; D010957:Plasmids; D015183:Restriction Mapping; D014466:Ultraviolet Rays / Q000009:adverse effects*
D008190:Lyases; D004255:Deoxyribodipyrimidine Photo-Lyase