Alpha- and beta-tubulin mRNAs of Trypanosoma cruzi originate from a single multicistronic transcript.
The cluster of alternated alpha- and beta-tubulin genes in the genome of Trypanosoma cruzi was shown to be transcribed into a single RNA molecule which upon processing gives rise to the mature alpha- and beta-tubulin mRNAs. This conclusion was based on: (i) nuclear RNA species with the same molecular mass hybridize to both alpha- and beta-tubulin cDNA probes; (ii) S1 nuclease assay of the clustered tubulin genes has shown protected DNA fragments of the same size and of greater molecular mass than that corresponding to the mRNAs, hybridizable to both alpha- and beta-tubulin cDNA probes; (iii) beta-tubulin hybrid selected RNA is still able to hybridize to alpha-tubulin probe.
Soares
C M
CM
Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brazil.
de Carvalho
E F
EF
Urményi
T P
TP
Carvalho
J F
JF
de Castro
F T
FT
Rondinelli
E
E
D000818:Animals; D015152:Blotting, Northern; D004247:DNA; D015342:DNA Probes; D009693:Nucleic Acid Hybridization; D012322:RNA Precursors; D012333:RNA, Messenger / Q000235:genetics*; D014158:Transcription, Genetic; D014349:Trypanosoma cruzi / Q000235:genetics*; D014404:Tubulin / Q000235:genetics*
D015342:DNA Probes; D012322:RNA Precursors; D012333:RNA, Messenger; D014404:Tubulin; D004247:DNA