title: Isolation from pig lens of two proteins with dihydrodiol dehydrogenase and aldehyde reductase activities.
abstract: Dimeric and monomeric proteins containing dihydrodiol dehydrogenase and aldehyde reductase activities were purified from pig lens. The dimeric enzyme of Mr 65,000 specifically oxidized the trans-dihydrodiols of naphthalene and benzene with NADP+ as a strict cofactor, and reduced alpha-diketones, aromatic aldehydes and glyceraldehyde with NADPH as a cofactor. The monomeric enzyme of Mr 35,000, although identical with aldose reductase, oxidized the trans-dihydrodiol of naphthalene at a pH optimum of 7.6. These results suggest that the two enzymes are involved in the pathogenesis of naphthalene cataract.
authors.affiliation: Department of Biochemistry, Gifu Pharmaceutical University, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Hara
authors.forename: T
authors.initials: T
authors.lastname: Harada
authors.forename: M
authors.initials: M
authors.lastname: Nakagawa
authors.forename: K
authors.initials: K
authors.lastname: Matsuura
authors.forename: T
authors.initials: T
authors.lastname: Nakayama
authors.forename: H
authors.initials: H
authors.lastname: Sawada
mesh_terms: D000426:Alcohol Dehydrogenase / Q000302:isolation & purification* / Q000378:metabolism; D000429:Alcohol Oxidoreductases / Q000302:isolation & purification* / Q000378:metabolism; D000818:Animals; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D007700:Kinetics; D007908:Lens, Crystalline / Q000201:enzymology*; D008970:Molecular Weight; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; D013379:Substrate Specificity; D013552:Swine
chemical_list: D010088:Oxidoreductases; D000429:Alcohol Oxidoreductases; C023125:dihydrodiol dehydrogenases; D000426:Alcohol Dehydrogenase; D044925:Oxidoreductases Acting on CH-CH Group Donors; C116788:cis-1,2-dihydro-1,2-dihydroxynaphthalene dehydrogenase