The influence of MHC-compatible and MHC-incompatible antigen-presenting cells on the survival of MHC-compatible cultured murine keratinocyte allografts.
Our group has shown previously that APC-depleted cultured epidermal keratinocytes show prolonged survival when grafted onto normal MHC-incompatible adult mice. We show here that in vitro culture also improves significantly the survival of MHC-compatible keratinocyte allografts, although these nonrejected grafts are repopulated by host cells identified by their dendritic morphology and phenotype (class II+, leukocyte-common antigen+) as APCs. Reconstitution of cultured grafts, immediately prior to transplantation, with MHC-compatible dendritic cells of either donor or recipient origin, results in graft rejection--thus demonstrating that cultured cells can be rejected by the recipient animal--and suggests that a paucity of APCs in the immediate postgrafting period is responsible for the privilege afforded these grafts.
Ramrakha
P S
PS
Department of Pathology, University of Cambridge, England.
Sharp
R J
RJ
Yeoman
H
H
Stanley
M A
MA
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology; D006084:Graft Rejection; D006085:Graft Survival; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007124:Immunoenzyme Techniques; D015603:Keratinocytes / Q000276:immunology / Q000637:transplantation*; D008285:Major Histocompatibility Complex; D051379:Mice; D008815:Mice, Inbred Strains
D000949:Histocompatibility Antigens Class II