A cis-acting transcription element of the c-myc gene can assume an H-DNA conformation.
I have used chemical probes and an oligonucleotide-association assay to determine the structure of a nuclease-sensitive, c-myc DNA region. I find that this DNA region can form a triplex-single stranded conformer in vitro--the H-DNA conformer. This DNA region has been shown previously to be a positive, cis-acting transcription element of the c-myc gene and to bind nuclear factors, including a base-paired ribonucleoprotein. Therefore, H-DNA may be a functionally important in vivo topoisomer where the H-DNA and B-DNA conformers have different transcriptional activities.
Kinniburgh
A J
AJ
Department of Human Genetics, Roswell Park Memorial Institute, Buffalo, NY 14263.
D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000648:ultrastructure; D004278:DNA, Superhelical / Q000187:drug effects; D004047:Diethyl Pyrocarbonate / Q000494:pharmacology; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009993:Osmium Tetroxide / Q000494:pharmacology; D010957:Plasmids; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc; D011519:Proto-Oncogenes; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic
D004278:DNA, Superhelical; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D014157:Transcription Factors; D004247:DNA; D004047:Diethyl Pyrocarbonate; D009993:Osmium Tetroxide