Rat skeletal muscle lysosomes contain glycogen.
1. A lysosome- and mitochondria-enriched fraction was obtained from rat skeletal muscle using a differential centrifugation procedure. This fraction contained a proportion (3-4%) of the tissue glycogen content. 2. Lysosomes and mitochondria were separated from one another by centrifugation of a cell-free extract upon discontinuous Ficoll-sucrose gradients. Little glycogen was associated with the resulting mitochondrial fraction. The lysosomal fraction, however, contained a significant amount of glycogen, accounting for 5% of the skeletal muscle glycogen. 3. The lysosomal glycogen was purified and found to be enriched in high molecular weight material. 4. The compartmentation of skeletal muscle glycogen metabolism is suggested.
Calder
P C
PC
Department of Biochemistry, University of Auckland, New Zealand.
Geddes
R
R
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D002498:Centrifugation; D002499:Centrifugation, Density Gradient; D055598:Chemical Phenomena; D002621:Chemistry; D005966:Glucuronidase / Q000378:metabolism; D006003:Glycogen / Q000302:isolation & purification*; D008247:Lysosomes / Q000032:analysis*; D008928:Mitochondria / Q000032:analysis; D008970:Molecular Weight; D009132:Muscles / Q000032:analysis*; D011506:Proteins / Q000378:metabolism; D051381:Rats; D000520:alpha-Glucosidases / Q000378:metabolism
D011506:Proteins; D006003:Glycogen; D000135:Acid Phosphatase; D000520:alpha-Glucosidases; D005966:Glucuronidase