title: [Immunoenzyme analysis of adenovirus hexon. Determination of antibodies from hyperimmunized chickens].
abstract: We have elaborated three systems of enzyme-linked immunosorbent assay (ELISA) for detection of chicken IgG antibodies specific for hexon antigens of three immunologically distinct adenovirus groups: those of mammalian adenoviruses (Mastadenovira), typical avian adenoviruses (Aviadenovira) and of egg-drop syndrome-76 (EDS-76) virus. In each system the antibodies against respective hexons were specifically detected. In mammalian adenovirus hexons the ELISA detects primarily the type-specific (epsilon) and genus-specific (alpha) antigenic determinants. The time course of anti-hexon antibodies content was followed during immunization. The level of anti-hexon antibodies in egg yolk reflects adequately their content in blood serum. The technique is suitable for serological diagnosis of chicken adenoviral infections as well as for characterization of egg-yolk antibodies obtained by preparative hyperimmunization of hens.
authors.forename: S N
authors.initials: SN
authors.lastname: Khil'ko
authors.forename: M A
authors.initials: MA
authors.lastname: Kirasova
authors.forename: S D
authors.initials: SD
authors.lastname: Piker
authors.forename: S D
authors.initials: SD
authors.lastname: Osidze
authors.forename: N V
authors.initials: NV
authors.lastname: Fomina
authors.forename: M P
authors.initials: MP
authors.lastname: Burgasova
authors.forename: T I
authors.initials: TI
authors.lastname: Tikhonenko
mesh_terms: D000256:Adenoviridae / Q000235:genetics* / Q000276:immunology; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D002645:Chickens; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D007114:Immunization; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000302:isolation & purification
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; D007074:Immunoglobulin G; C018315:hexon capsid protein, Adenovirus
vernacular_title: Immunfermentnyĭ analiz geksona adenovirusov. Opredelenie antitel ot giperimmunizirovannykh kur.