Curing of the killer character of Saccharomyces cerevisiae with acridine orange.
Acridine orange, an intercalating dye usually employed in the curing of bacterial plasmids, was tested for its ability to cure K1 and K2 killer strains (laboratory and wine strains). The results showed a high curing percentage of the killer character. This was demonstrated by the loss of M1 or M2 dsRNAs (responsible for toxin production and resistance to it) and because the meiotic products exhibited non-Mendelian segregation. The curing percentages varied, depending on the strain but not on the killer type, and showed similar efficiency as compared with other known curing agents.
Cansado
J
J
Departamento de Microbiologia, Facultad de Farmacia, Universidad de Santiago de Compostela, Spain.
Longo
E
E
Agrelo
D
D
Villa
T G
TG
D000165:Acridine Orange / Q000494:pharmacology*; D004587:Electrophoresis, Agar Gel; D055646:Killer Factors, Yeast; D009183:Mycotoxins / Q000235:genetics*; D010641:Phenotype; D010957:Plasmids; D012330:RNA, Double-Stranded / Q000235:genetics; D012331:RNA, Fungal / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins
D055646:Killer Factors, Yeast; D009183:Mycotoxins; D012330:RNA, Double-Stranded; D012331:RNA, Fungal; D029701:Saccharomyces cerevisiae Proteins; D000165:Acridine Orange