title: New crystal forms of the small subunit of ribonucleotide reductase from Escherichia coli.
abstract: The small subunit of ribonucleotide reductase from Escherichia coli has been crystallized in two new crystal forms. The form most suitable for X-ray analysis belongs to the orthorhombic space group P2(1)2(1)2(1). It has the cell dimensions 74.3 A, 85.5 A, 115.7 A and diffracts to about 2.1 A resolution. The asymmetric unit most probably contains one dimer. Absorption spectra of single crystals confirm that the crystals contain a binuclear iron center. Crystals of the iron-depleted apoenzyme have also been obtained.
authors.affiliation: Department of Molecular Biology, Swedish University of Agricultural Sciences, Uppsala.
authors.forename: P
authors.initials: P
authors.lastname: Nordlund
authors.forename: U
authors.initials: U
authors.lastname: Uhlin
authors.forename: C
authors.initials: C
authors.lastname: Westergren
authors.forename: T
authors.initials: T
authors.lastname: Joelsen
authors.forename: B M
authors.initials: BM
authors.lastname: Sjöberg
authors.forename: H
authors.initials: H
authors.lastname: Eklund
mesh_terms: D003460:Crystallization; D004926:Escherichia coli / Q000201:enzymology*; D007700:Kinetics; D011487:Protein Conformation; D012264:Ribonucleotide Reductases / Q000302:isolation & purification / Q000378:metabolism; D014961:X-Ray Diffraction
chemical_list: D012264:Ribonucleotide Reductases