Gastrin-releasing peptide-like immunoreactivity in porcine follicular fluid and ovary.
Porcine follicular fluid was found to contain gastrin-releasing peptide (GRP) by radioimmunoassay. High pressure liquid chromatography of the extracted material showed two peaks of GRP immunoreactivity that closely resembled the C-terminal fragments of GRP, GRP18-27 and GRP14-27. Immunohistochemical studies revealed specific staining for GRP in the granulosa cells of adult porcine ovary. These results demonstrate the presence of substances which behave like authentic GRP-like peptides in porcine ovary and follicular fluid and suggest that these peptides may play a paracrine and/or autocrine role in the regulation of the ovarian function.
Gnessi
L
L
V Medical Clinic, Policlinico Umberto I, University of Rome La Sapienza, Italy.
Altamura
S
S
Arizzi
M
M
Tei
C
C
La Torre
C
C
Bonifacio
V
V
Facchinetti
F
F
Spera
G
G
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005260:Female; D015571:Follicular Fluid / Q000032:analysis*; D019886:Gastrin-Releasing Peptide; D007124:Immunoenzyme Techniques; D010053:Ovary / Q000032:analysis*; D010455:Peptides / Q000032:analysis*; D011863:Radioimmunoassay; D013552:Swine
D010455:Peptides; D019886:Gastrin-Releasing Peptide