title: Two different anti-erythroid monoclonal antibodies in immunodiagnosis of human leukemias: a comparative study.
abstract: To date, only anti-glycophorin-A monoclonal antibodies (MAbs) have been widely used as anti-erythroid probes in the diagnosis of leukemias. We have examined blood, bone-marrow and lymph-node samples from 474 patients, adults and children, with different hemopoietic malignancies, using a panel of MAbs including 2 anti-erythroid MAbs directed to glycophorin-A and an antigen of erythroblasts, Ag-Eb. MAb HAE9 directed against a human epitope of Ag-Eb has earlier been shown to be highly specific for immature erythroid cells. Of all the patients, 2.7% demonstrated glycophorin-A expression on blast cells, while anti-Ag-Eb MAb HAE9 reacted positively with cells from 6.0% of patients. Samples from 31 of 474 (6.5%) patients expressed one or both erythroid markers. Our results indicate that MAb HAE9 may be useful, in combination with anti-glycophorin-A MAbs, as an anti-erythroid probe for immunophenotyping human leukemias.
authors.affiliation: Cancer Research Center, Moscow, USSR.
authors.forename: N N
authors.initials: NN
authors.lastname: Tupitsyn
authors.forename: E B
authors.initials: EB
authors.lastname: Mechetner
authors.lastname: Baryschnikov AJu
authors.forename: T S
authors.initials: TS
authors.lastname: Drozdova
authors.forename: M A
authors.initials: MA
authors.lastname: Frenkel
authors.forename: E S
authors.initials: ES
authors.lastname: Ievleva
authors.forename: A V
authors.initials: AV
authors.lastname: Kiselev
authors.forename: A A
authors.initials: AA
authors.lastname: Perilov
authors.forename: I S
authors.initials: IS
authors.lastname: Peterson
authors.forename: N A
authors.initials: NA
authors.lastname: Probatova
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000951:Antigens, Neoplasm / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D014408:Biomarkers, Tumor / Q000032:analysis; D001853:Bone Marrow / Q000276:immunology; D002648:Child; D015672:Erythroid Precursor Cells / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007159:Immunologic Tests / Q000379:methods; D007938:Leukemia / Q000175:diagnosis*; D008198:Lymph Nodes / Q000276:immunology; D010641:Phenotype
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D014408:Biomarkers, Tumor