Advantages of a new agar medium in the primary isolation of Mycobacterium bovis.
Suspect tuberculous lesions from 116 cattle were examined histologically and cultured for Mycobacterium bovis using 5 different media. The media used were: B83, an agar medium incorporating bovine blood and sodium pyruvate; Middlebrook's agar; 2 variations of Stonebrink's medium; Löwenstein-Jensen medium. The B83 medium and a modification of Stonebrink's medium which had a lowered concentration of malachite green were most successful, detecting 95.2% of tuberculous animals when used together. The B83 medium detected isolates approximately 1 week earlier and had more colonies than the Stonebrink's modification. A combination of 2 slopes of B83 and 2 slopes of modified Stonebrink's medium is recommended for routine culture of samples.
Cousins
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DV
Animal Health Laboratories, Department of Agriculture, South Perth, W.A., Australia.
Francis
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BR
Gow
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BL
D000362:Agar; D000818:Animals; D002417:Cattle; D003470:Culture Media; D009163:Mycobacterium bovis / Q000254:growth & development / Q000302:isolation & purification*; D014380:Tuberculosis, Bovine / Q000382:microbiology*
D003470:Culture Media; D000362:Agar