title: Regulation of a bifunctional mRNA results in synthesis of secreted and nuclear probasin.
abstract: Probasin, a rat prostatic protein, is statistically related to members of a protein family that includes serum, cellular, and nuclear proteins. In vivo, probasin appears both in the secretions and in the nucleus of prostatic epithelial cells. Using primer extension and S1 nuclease protection assays we detected only one probasin mRNA. Thus, the localization of this protein to two separate cellular regions must be encoded by this one mRNA. Furthermore, in vitro translation of synthetic probasin mRNA demonstrated that a protein containing a signal peptide and a protein lacking a signal peptide were synthesized by initiation at different AUG codons. These data are consistent with a mechanism of translational regulation of a eukaryotic bifunctional mRNA.
authors.affiliation: Department of Physiology, University of Manitoba, Winnipeg, Canada.
authors.forename: A M
authors.initials: AM
authors.lastname: Spence
authors.forename: P C
authors.initials: PC
authors.lastname: Sheppard
authors.forename: J R
authors.initials: JR
authors.lastname: Davie
authors.forename: Y
authors.initials: Y
authors.lastname: Matuo
authors.forename: N
authors.initials: N
authors.lastname: Nishi
authors.forename: W L
authors.initials: WL
authors.lastname: McKeehan
authors.forename: J G
authors.initials: JG
authors.lastname: Dodd
authors.forename: R J
authors.initials: RJ
authors.lastname: Matusik
mesh_terms: D000595:Amino Acid Sequence; D000727:Androgen-Binding Protein / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism*; D003001:Cloning, Molecular; D003062:Codon / Q000235:genetics; D005796:Genes; D005809:Genes, Regulator; D007124:Immunoenzyme Techniques; D008297:Male; D008969:Molecular Sequence Data; D011467:Prostate / Q000378:metabolism*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D012689:Sequence Homology, Nucleic Acid
chemical_list: D000727:Androgen-Binding Protein; D003062:Codon; D012333:RNA, Messenger; C027519:probasin