Antibody specificity in the immune response to insulin.
The immune response to insulin in Balb/c mice has been investigated with regard to the fine specificity of antibodies present during the primary stage of immune responsiveness. Hybridoma technology was used to raise monoclonal antibodies and a solid phase enzyme-linked immunoassay to test their specificity for human, bovine and porcine insulins. Heteroclitic antibodies were observed. These showed greater binding to the cross-reactive bovine and porcine insulins than to the inducing human insulin.
Mirza
I H
IH
Department Medicine II, Southampton General Hospital, UK.
Wilkin
T J
TJ
D000818:Animals; D000910:Antibodies, Heterophile / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000915:Antibody Affinity; D000918:Antibody Specificity; D002417:Cattle; D003429:Cross Reactions; D005260:Female; D006801:Humans; D007156:Immunologic Memory; D007328:Insulin / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013045:Species Specificity; D013552:Swine
D000910:Antibodies, Heterophile; D000911:Antibodies, Monoclonal; D007328:Insulin