Characterization in rat pancreatic juice of a protein homologous to the human pancreatic stone protein.
1. The pancreatic stone protein (PSP, Mr 15,000) which has been discovered in human calculi derives from the native glycosylated forms of the protein (Mrs 17,500-22,000) which are present in human pancreatic juice through tryptic cleavage of the Arg 11-Ile 12 bond. 2. In the present study, a homologous native form of the protein (Mr 17,000) was purified from rat pancreatic juice. 3. Its N-terminal amino acid sequence was found to display a high degree of homology with that of the human native protein forms, apart from the fact that it was not glycosylated. 4. In rat as in human, tryptic cleavage of the Arg 11-Ile 12 bond transforms a soluble protein into one which is practically insoluble at neutral pH.
Adrich
Z
Z
Groupe de Biochimie Fondamentale, Unité de Recherches de Physiologie et Pathologie Digestives, Marseille, France.
De Caro
A M
AM
Guidoni
A A
AA
Woudstra
M E
ME
Rovery
M
M
D000595:Amino Acid Sequence; D000818:Animals; D002135:Calcium-Binding Proteins / Q000302:isolation & purification*; D002241:Carbohydrates / Q000032:analysis; D006801:Humans; D051847:Lithostathine; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D010189:Pancreatic Juice / Q000032:analysis*; D010447:Peptide Hydrolases / Q000378:metabolism; D010455:Peptides / Q000032:analysis; D011817:Rabbits; D051381:Rats; D012995:Solubility
D002135:Calcium-Binding Proteins; D002241:Carbohydrates; D051847:Lithostathine; D009419:Nerve Tissue Proteins; D010455:Peptides; C497617:REG1A protein, human; C497616:Reg1a protein, rat; D010447:Peptide Hydrolases