Activation of the K-ras oncogene in liver tumors of Hudson River tomcod.
Adult Atlantic tomcod collected from the Hudson River slightly north of New York City have an extremely high incidence (55-90%) of histologically defined hepatocellular carcinomas, whereas tomcod from control sites in Maine or Rhode Island exhibit little evidence of this condition. Genomic DNA was isolated from Hudson tomcod tumors and from normal Hudson and Saco River, Maine tomcod livers and tested for transforming activity in the NIH3T3 transfection assay. Six out of nine tumors (66%) tested proved positive. Southern blot analysis of all primary (6/6) transfectant and nude mouse tumor DNAs revealed evidence of an exogenous tomcod K-ras gene, while no activation of the H-ras gene was observed. These studies demonstrate that an outbred population of fishes and inbred mammals suffer genetic alternations at the same oncogene loci and suggest that similar pathways to neoplasia may be operative in both systems. Oncogene activation in naturally exposed feral populations may prove a particularly sensitive marker of environmental degradation in aquatic systems.
Wirgin
I
I
Institute of Environmental Medicine, New York University Medical Center, NY 10016.
Currie
D
D
Garte
S J
SJ
D000818:Animals; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D005399:Fishes; D005618:Fresh Water; D015972:Gene Expression Regulation, Neoplastic; D011905:Genes, ras; D008113:Liver Neoplasms / Q000235:genetics*; D051379:Mice; D008819:Mice, Nude; D014162:Transfection