[Cloning and study of the function of recA-like gene of Yersinia pestis in Escherichia coli cells].
A 2 kb fragment of Yersinia pestis genome cloned in Escherichia coli cells of the strain HB101 contains a gene able to complement the recA-dependent deficiency of E. coli cells in UV-resistance, resistance to alkylating agents, UV- and MNNG-induced mutability. Cellular capability for homologous recombination in crosses with HfrH donor, derepressed synthesis of bacteriocins (colicin E1 and pesticin 1) is also complemented by the fragment in E. coli recA-strains. The obtained data suggest the functional homology of the cloned recA-like gene product with the product of E. coli recA-gene.
Suchkov
I Iu
IIu
Mishan'kin
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BN
D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development; D005798:Genes, Bacterial; D009154:Mutation; D010957:Plasmids; D011938:Rec A Recombinases / Q000235:genetics*; D015010:Yersinia pestis / Q000235:genetics* / Q000254:growth & development
D011938:Rec A Recombinases
Klonirovanie i issledovnie funktsii recA-podobnogo gena Yersinia pestis v kletkakh kishechnoĭ palochki.