title: A DNA binding protein that recognizes oligo(dA).oligo(dT) tracts.
abstract: Oligo(dA).oligo(dT) tracts are common intergenic sequences in many organisms. In the yeast Saccharomyces cerevisiae, these sequences have been shown to influence transcription of adjacent genes. We have purified an oligo(dA).oligo(dT)-binding protein from S. cerevisiae and cloned its gene. This protein, which has been named datin, requires at least 9-11 bp of oligo(dA).oligo(dT) DNA for high affinity binding. The gene for datin (the DAT gene) encodes a 248-residue protein which contains a number of repeated sequence motifs. Datin purified from yeast corresponds to the N-terminal half of the DAT gene product. Null mutants in the DAT gene are viable but phenotypically distinguishable from congenic wild-type strains. We discuss unusual structural features and biochemical properties of datin in relation to its possible functions.
authors.affiliation: Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
authors.forename: E
authors.initials: E
authors.lastname: Winter
authors.forename: A
authors.initials: A
authors.lastname: Varshavsky
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D050483:Dopamine Plasma Membrane Transport Proteins; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005800:Genes, Fungal; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D009154:Mutation; D009419:Nerve Tissue Proteins; D009838:Oligodeoxyribonucleotides / Q000378:metabolism*; D011067:Poly dA-dT / Q000378:metabolism*; D011089:Polydeoxyribonucleotides / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D014158:Transcription, Genetic
chemical_list: C060858:DAT1 protein, S cerevisiae; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D050483:Dopamine Plasma Membrane Transport Proteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D009838:Oligodeoxyribonucleotides; D011089:Polydeoxyribonucleotides; D029701:Saccharomyces cerevisiae Proteins; C016023:oligo(dA-dT); D011067:Poly dA-dT