title: Participation of the hup gene product in site-specific DNA inversion in Escherichia coli.
abstract: The closely related Escherichia coli genes hupA and hupB each encode a bacterial histone-like protein HU. We report here that DNA inversion mediated by hin, gin, pin and rci but not by cin is blocked in a hupA hupB double mutant, although inversions in these systems occur in the hupA or hupB single mutant as efficiently as in the wild-type strain. These findings show that HU protein participates in site-specific DNA inversion in E. coli and that only one subunit, either HU-1 or HU-2, is sufficient for this inversion.
authors.affiliation: Laboratory of Molecular Genetics, Riken, Tsukuba Life Science Center, Ibaraki, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Wada
authors.forename: K
authors.initials: K
authors.lastname: Kutsukake
authors.forename: T
authors.initials: T
authors.lastname: Komano
authors.forename: F
authors.initials: F
authors.lastname: Imamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Kano
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D007446:Chromosome Inversion; D004254:DNA Nucleotidyltransferases / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D009154:Mutation; D010957:Plasmids; D011995:Recombination, Genetic; D014170:Transformation, Genetic
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C040330:histone-like protein HU, bacteria; D004254:DNA Nucleotidyltransferases