Crystallization of alcohol oxidase from Pichia pastoris.
Crystals of alcohol oxidase purified from Pichia pastoris were grown in microdialysis buttons in a solution of polyethylene glycol, sodium chloride and sodium azide. The crystals were stratified along the major axis and up to 3 mm in length. X-ray diffraction experiments indicated a space group of P2(1) and unit cell dimensions of a = 157.3 A, b = 171.5 A and c = 231.6 A. Crystals diffract to beyond 2.7 A and are suitable for X-ray structure analysis.
Boys
C W
CW
Astbury Department of Biophysics, University of Leeds, U.K.
Hill
D J
DJ
Stockley
P G
PG
Woodward
J R
JR
D000429:Alcohol Oxidoreductases; D003460:Crystallization; D010843:Pichia / Q000201:enzymology*; D004718:Saccharomycetales / Q000201:enzymology*; D014961:X-Ray Diffraction
D000429:Alcohol Oxidoreductases; C019643:alcohol oxidase