title: [Methodologic aspects of immunoperoxidase technics].
abstract: In attempt to standardize immunoperoxidase tests many partial methodological experiments were performed and the results discussed. The proved processing included purification of peroxidase conjugates, sequential administering in double indirect immunoperoxidase method (double sandwich). A high level dilution of primary antibodies and lengthening of incubation were recommended. Silicagel and vaseline rim round sections were found advantageous for improving adhesion of paraffin sections.
authors.forename: P
authors.initials: P
authors.lastname: Capek
authors.forename: L
authors.initials: L
authors.lastname: Rozprimová
authors.forename: M
authors.initials: M
authors.lastname: Kolárová
mesh_terms: D007124:Immunoenzyme Techniques
vernacular_title: Metodické aspekty imunoperoxidázových technik.