Neuronal imaging with colloidal gold.
Colloidal gold is easily prepared, and readily adsorbs to a number of immunoreagents and other proteins for a wide variety of uses for neuronal visualization. Gold probes serve a role as immunolabels for both light and electron microscopy. As an ultrastructural immunocytochemical marker for detection of proteins, peptides or amino acids, gold can be used for immunostaining thick or thin sections prior to embedding, or for immunostaining ultrathin sections after embedding tissue in conventional or unusual embedding matrices. By virtue of its particulate nature, gold as an immunolabel facilitates a semi-quantitative analysis of relative antigen densities on ultrathin sections. Various combinations of different size gold particles or dual immunolabelling with enzymatic immunolabels together with colloidal gold or silver-intensified gold serve well for ultrastructural immunocytochemical localization of two antigens in the same tissue section. Colloidal gold can be detected with light microscopy, transmission and scanning electron microscopy, and with confocal laser microscopy. Silver intensification allows detection of gold at both the light and electron microscope level, and increases the sensitivity of immunogold procedures. Colloidal gold is useful as a tracer for physiological studies of transport and internalization in neurons in vivo and in vitro; computer-assisted video imaging techniques allow detection and tracking of single gold particles in living cells.
van den Pol
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Section of Neurosurgery, Yale University School of Medicine, New Haven, Ct. 06510.
D000818:Animals; D003102:Colloids; D005455:Fluorescent Antibody Technique; D006046:Gold; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008853:Microscopy; D008854:Microscopy, Electron; D009474:Neurons / Q000648:ultrastructure*
D003102:Colloids; D006046:Gold