title: [Construction of a family of artificial genes of human insulin].
abstract: Using oligonucleotide-directed mutagenesis and chemical-enzymatic DNA synthesis, genes for A and B insulin chains, C-peptide and Tyr-C-peptide have been constructed starting from synthetic gene for human proinsulin synthesized earlier. The genes for human preproinsulin, mini-proinsulin, single-chain insulin and their modifications were also synthesized. The constructions obtained were cloned in plasmid vectors.
authors.forename: V A
authors.initials: VA
authors.lastname: Efimov
authors.forename: A A
authors.initials: AA
authors.lastname: Buriakova
authors.forename: I N
authors.initials: IN
authors.lastname: Pashkova
authors.forename: N N
authors.initials: NN
authors.lastname: Polushin
authors.forename: O G
authors.initials: OG
authors.lastname: Chakhmakhcheva
mesh_terms: D000595:Amino Acid Sequence; D004926:Escherichia coli / Q000235:genetics; D005813:Genes, Synthetic; D005818:Genetic Engineering; D006801:Humans; D007328:Insulin / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D011384:Proinsulin / Q000235:genetics; D011498:Protein Precursors / Q000235:genetics
chemical_list: D007328:Insulin; D011498:Protein Precursors; C013737:preproinsulin; D011384:Proinsulin
vernacular_title: Konstruirovanie semeĭstva iskusstvennykh genov insulina cheloveka.