[The possibilities of using variants of immunoenzyme analysis for detecting L-form Salmonella typhi bacteria in biological fluids].
The possibility of using, on principle, the enzyme immunoassay (EIA) in different modifications for the detection of S. typhi L-forms in biological fluids (blood, urine) was established. The inhibiting variant of EIA showed the highest sensitivity: 1 ng/ml. The direct sandwich variant permitted the quantitative determination of the antigen of S. typhi L-form in the widest range of 20-500 ng/ml. The indirect enzyme immunometric variant permitted the detection of S. typhi L-forms with a sensitivity of 10(5) colony-forming units per ml only in urine.
Gorelov
A L
AL
Levina
G A
GA
Prozorovskiĭ
S V
SV
D000942:Antigens, Bacterial / Q000032:analysis*; D001826:Body Fluids / Q000276:immunology*; D005069:Evaluation Studies as Topic; D006801:Humans; D007124:Immunoenzyme Techniques; D007740:L Forms / Q000276:immunology*; D012485:Salmonella typhi / Q000276:immunology*; D012995:Solubility
D000942:Antigens, Bacterial
Izuchenie vozmozhnosti ispol'zovaniia razlichnykh variantov immunofermentnogo analiza dlia vyiavleniia L-form briushno-tifoznykh bakteriĭ v biologicheskikh zhidkostiakh.