Optimization of tetrachlorosalicylanilide and ultraviolet A doses at sensitization and challenge for contact photosensitivity in the mouse.
We studied contact photosensitivity (CPS) to tetrachlorosalicylanilide (TCSA) in BALB/cJ mice with various doses of TCSA and UVA at sensitization and challenge. From these studies we recommend the following protocol: sensitization on days 0 and 1 with 50 microliters of 1% TCSA followed by 3 J/cm2 of UVA, and challenge on day 7 with 10 microliters of 3% TCSA followed by 6 J/cm2 of UVA. This gave an ear swelling response of 27.4 +/- 0.6 x 10(-3) (mean +/- standard error). We also demonstrated that there is reciprocity between volume and concentration of TCSA at sensitization but not between TCSA and UVA doses at sensitization.
Brown
W R
WR
Division of Dermatology, University of Toronto, Ontario, Canada.
Furukawa
R D
RD
Shivji
G M
GM
Ramsay
C A
CA
D000485:Allergens; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D005260:Female; D007114:Immunization; D007158:Immunologic Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D009994:Osmolar Concentration; D010787:Photosensitivity Disorders / Q000276:immunology*; D012457:Salicylamides / Q000276:immunology*; D012458:Salicylanilides / Q000276:immunology*; D014466:Ultraviolet Rays
D000485:Allergens; D012457:Salicylamides; D012458:Salicylanilides; C003390:3,3',4',5-tetrachlorosalicylanilide