Secretion of islet amyloid polypeptide in response to glucose.
The content of islet amyloid polypeptide (IAPP) in isolated rat pancreatic islets was determined by a radioimmunoassay. Reverse-phase high-performance liquid chromatography analysis revealed that a main peak of IAPP immunoreactivity in the extracts from the islets corresponded to a synthetic rat IAPP. Secretion of IAPP from the cells is regulated by the extracellular glucose concentration. Thus, IAPP may be a novel regulator for glucose homeostasis and changes in the secretion perhaps relate to insular amyloid deposits and impaired glucose tolerance in type 2 diabetes mellitus.
Kanatsuka
A
A
Second Department of Internal Medicine, Chiba University School of Medicine, Japan.
Makino
H
H
Ohsawa
H
H
Tokuyama
Y
Y
Yamaguchi
T
T
Yoshida
S
S
Adachi
M
M
D000682:Amyloid / Q000378:metabolism*; D000818:Animals; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology*; D066298:In Vitro Techniques; D058228:Islet Amyloid Polypeptide; D007515:Islets of Langerhans / Q000378:metabolism*; D008297:Male; D011863:Radioimmunoassay; D051381:Rats; D012636:Secretory Rate / Q000187:drug effects
D000682:Amyloid; D058228:Islet Amyloid Polypeptide; D005947:Glucose