Effects of glycolytic and cytoskeletal inhibitors on phagocytic and nitroblue tetrazolium reductive activities of bovine neutrophils.
Phagocytic and oxidative metabolic activities of bovine blood neutrophils were determined in the presence of glycolytic (NaF) and cytoskeletal (colchicine, cytochalasin B, and prostaglandin E1) inhibitors. Phagocytosis and post-phagocytic oxidative metabolic activity, measured by nitroblue tetrazolium reduction, were determined using zymosan, Escherichia coli, Staphylococcus aureus, or Streptococcus agalactiae. Sodium fluoride (1.25 microM to 1.25 mM concentrations) did not significantly (P greater than 0.05) inhibit phagocytosis of S aureus and Str agalactiae, whereas phagocytosis of zymosan and E coli was significantly (P less than 0.05) inhibited only at 1.25 mM concentration. Colchicine at 1.25 nM to 1.25 microM concentrations significantly inhibited phagocytosis of zymosan and E coli, but not of S aureus and Str agalactiae. Cytochalasin B at 1.25 nM to 1.25 microM concentrations significantly inhibited phagocytosis of zymosan and all 3 bacteria, whereas prostaglandin E1 was noninhibitory at similar concentrations. Nitroblue tetrazolium reduction, in general, was not significantly affected by NaF and cytoskeletal inhibitors.
Silva
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Department of Clinical Pathology, School of Veterinary Medicine, University of California, Davis 95616.
Jain
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D000527:Alprostadil / Q000494:pharmacology*; D000818:Animals; D002417:Cattle / Q000097:blood; D003078:Colchicine / Q000494:pharmacology*; D003571:Cytochalasin B / Q000494:pharmacology*; D004734:Energy Metabolism / Q000187:drug effects; D004926:Escherichia coli / Q000276:immunology; D006019:Glycolysis; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D010084:Oxidation-Reduction; D010587:Phagocytosis / Q000187:drug effects; D012969:Sodium Fluoride / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000276:immunology; D013292:Streptococcus agalactiae / Q000276:immunology
D009580:Nitroblue Tetrazolium; D003571:Cytochalasin B; D012969:Sodium Fluoride; D000527:Alprostadil; D003078:Colchicine