title: Single-stranded circular DNA generated from broad host range plasmid R1162 and its derivatives.
abstract: Extraction of R1162 plasmid DNA with the alkaline lysis method yields considerable amounts of single-stranded circular plasmid DNA. Destabilization of plasmid DNA is stimulated by the R1162 mob region in cis. The formation of single-stranded circular DNA is initiated at a specific site on the plasmid, presumably the origin of transfer (oriT).
authors.affiliation: Groningen Biotechnology Center, The Netherlands.
authors.forename: M
authors.initials: M
authors.lastname: Kok
authors.forename: A C
authors.initials: AC
authors.lastname: Arnberg
authors.forename: B
authors.initials: B
authors.lastname: Witholt
mesh_terms: D004270:DNA, Circular / Q000235:genetics / Q000648:ultrastructure; D004277:DNA, Single-Stranded / Q000235:genetics* / Q000648:ultrastructure; D004926:Escherichia coli / Q000235:genetics; D008854:Microscopy, Electron; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D011815:R Factors
chemical_list: D004270:DNA, Circular; D004277:DNA, Single-Stranded