[Detection of HLA-A and HLA-B antigens on thrombocytes. Comparison of 3 methods].
HLA antigens A1, 2, 9, 10, B5, 7, 8, 12, 35 were determined on 28 samples of healthy persons' thrombocytes by means of three tests; the microabsorption and immunofluorescence tests and the ELISA technique. Identification with the ELISA test was exceptional. Unlike the microabsorption test, the immunofluorescence test was employed to identify HLA antigens with only some sera as a rule. Identical positive results of both the microabsorption and the immunofluorescence tests were rather infrequent. The authors could not corroborate literary reports on difficulties in the identification of the antigens HLA-B8 and HLA-B12, certain problems were encountered in the case of the HLA-B7 antigen only. A discussion is presented of the causes of the different results of each of the tests, and of the relevance of the results for transfusions of platelet concentrates chosen on the basis of lymphocytic cross-matching.
Májský
A
A
Korínková
P
P
D001792:Blood Platelets / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D015234:HLA-A Antigens / Q000032:analysis*; D015235:HLA-B Antigens / Q000032:analysis*; D006801:Humans; D007163:Immunosorbent Techniques
D015234:HLA-A Antigens; D015235:HLA-B Antigens
Průkaz HLA-A, B antigenů na trombocytech. Srovnání trí metod.