title: Differential recognition of two cloned Brugia malayi antigens by antibody class.
abstract: The humoral and cellular immune response to filarial parasites is complex. Numerous studies have shown that antibodies to a large number of protein and non-protein antigens may be produced over the course of infection and that immune recognition of any given antigen may vary by disease manifestation and by immunoglobulin class. We have used the techniques of molecular cloning to attempt to dissect this complex interaction, and describe here two clones, isolated from an expression library constructed from Brugia malayi genomic DNA, whose products are recognized by distinct immunoglobulin classes. A lambda gt11 fusion protein containing part of the B. malayi myosin tail region is recognized by antibodies of the IgG class from a high percentage of bancroftian filariasis patients. A fusion protein containing a collagen-like sequence is less frequently and weakly recognized under the same experimental conditions, but is almost universally recognized when the developing reagent is specific for IgE. We thus identify specific filarial proteins against which the infected human host responds preferentially with antibodies of a specific immunoglobulin class.
authors.affiliation: Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY.
authors.forename: C
authors.initials: C
authors.lastname: Werner
authors.forename: G I
authors.initials: GI
authors.lastname: Higashi
authors.forename: J A
authors.initials: JA
authors.lastname: Yates
authors.forename: T V
authors.initials: TV
authors.lastname: Rajan
mesh_terms: D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000145:classification / Q000276:immunology*; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D002009:Brugia / Q000235:genetics / Q000276:immunology*; D002648:Child; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004605:Elephantiasis, Filarial / Q000276:immunology*; D005260:Female; D005368:Filariasis / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000276:immunology; D012689:Sequence Homology, Nucleic Acid; D014957:Wuchereria / Q000276:immunology*; D014958:Wuchereria bancrofti / Q000235:genetics / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G; D011993:Recombinant Fusion Proteins; D007073:Immunoglobulin E; D004247:DNA