von Willebrand factor multimer analysis using a sensitive peroxidase staining method.
A peroxidase staining method for von Willebrand factor (vWF) multimer analysis was modified for comparison with an autoradiographic method. This method consists of SDS-agarose electrophoresis followed by blotting to a nitrocellulose membrane and a sensitive peroxidase staining method. The resolution of vWF multimers on the nitrocellulose membrane is comparable to that on the conventional autoradiography. Results can be obtained in 3 days. This nonradioisotopic method will be useful for the determination of the type of von Willebrand's disease in clinical laboratories.
Tomita
Y
Y
Department of Pediatrics, University of California, Davis School of Medicine.
Harrison
J
J
Abildgaard
C F
CF
D001345:Autoradiography; D001704:Biopolymers; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007124:Immunoenzyme Techniques; D014841:von Willebrand Factor / Q000032:analysis*
D001704:Biopolymers; D014841:von Willebrand Factor