Expression of Yb1 glutathione S-transferase using a baculovirus expression system.
A full-length cDNA clone was isolated for rat liver Yb1 glutathione S-transferase (EC 2.5.1.18). The coding sequence of Yb1 cDNA was inserted into a baculovirus vector for infection of Spodoptera frugiperda (SF9) cells. The enzymatically active recombinant Yb1 glutathione S-transferase protein has a native molecular weight of 42,000 daltons (by molecular sieve chromatography), a subunit molecular weight of 26,500 daltons (by SDS-polyacrylamide gel electrophoresis), a pI of 8.4 and an extinction coefficient E1%280 of 5.6 +/- 0.4.
Hsieh
J C
JC
Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, Republic of China.
Liu
L F
LF
Chen
W L
WL
Tam
M F
MF
D000818:Animals; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D005982:Glutathione Transferase / Q000235:genetics* / Q000378:metabolism; D007304:Insect Viruses / Q000235:genetics*; D010957:Plasmids; D051381:Rats; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D013379:Substrate Specificity
D011994:Recombinant Proteins; D004247:DNA; D005982:Glutathione Transferase