Detection of viral infection by immunofluorescence in formalin-fixed tissues, pretreated with trypsin.
The presence of viral antigen in sections from formalin-fixed and paraffin-embedded human tissues was demonstrated by trypsin digestion followed by direct or indirect immunofluorescence. The specimens may be used for retrospective diagnosis. The immunofluorescence technique has to be adapted to the suspected virus infection on the basis of previous histopathologic study. Variations of trypsin concentration time and temperature of incubation, expose different viral antigens and have to be previously tested for each unknown system. For measles virus detection in lung a stronger digestion has to be applied as compared to adenovirus or respiratory disease viruses in the same tissue. Flavivirus in liver tissue needs a weaker digestion. The reproducibility of the method makes it useful as a routine technique in diagnosis of virus infection.
Barth
O M
OM
Instituto Oswaldo Cruz, Departamento de Virologia, Rio de Janeiro, Brasil.
Majerowicz
S
S
Menasce
L P
LP
Schatzmayr
H G
HG
D005455:Fluorescent Antibody Technique; D006801:Humans; D014357:Trypsin / Q000378:metabolism*; D014776:Virus Cultivation; D014777:Virus Diseases / Q000175:diagnosis*
D014357:Trypsin