Endothelin stimulates a sustained 1,2-diacylglycerol increase and protein kinase C activation in bovine aortic smooth muscle cells.
Endothelin is a long-lasting potent vasoconstrictor peptide. We report here that in bovine aortic smooth muscle cells, endothelin biphasically increased total cellular diacylglycerol (DAG) content. When cellular DAG was labeled with [14C] glycerol for 48h, endothelin stimulated [14C]DAG formation in a biphasic pattern. Only one prolonged phase of DAG accumulation was observed when cells were labeled with [3H]glycerol for 2 h. Endothelin induced an increase in the membranous protein kinase C (PKC) activities, which lasted for more than 20 min. These data suggest that (i) endothelin stimulates a sustained generation of DAG, (ii) this accumulation of DAG results in a sustained translocation of cytosolic PKC activities to the membrane.
Lee
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Joslin Diabetes Center, Brigham & Women's Hospital, Boston, Massachusetts 02215.
Chao
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Hu
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King
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D000818:Animals; D001011:Aorta; D002250:Carbon Radioisotopes; D002417:Cattle; D002478:Cells, Cultured; D004075:Diglycerides / Q000096:biosynthesis*; D016232:Endothelins; D004789:Enzyme Activation; D005989:Glycerides / Q000096:biosynthesis*; D005990:Glycerol / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D009131:Muscle, Smooth, Vascular / Q000201:enzymology / Q000378:metabolism*; D010455:Peptides / Q000494:pharmacology*; D011493:Protein Kinase C / Q000378:metabolism*; D014316:Tritium
C011439:1,2-diacylglycerol; D002250:Carbon Radioisotopes; D004075:Diglycerides; D016232:Endothelins; D005989:Glycerides; D010455:Peptides; D014316:Tritium; D011493:Protein Kinase C; D005990:Glycerol