Local adoptive transfer of delayed-type hypersensitivity to tuberculin from M. bovis (BCG) infected mice.
Immunological properties of the cells mediating delayed-type hypersensitvity to tuberculin and genetic requirements of this reaction have been studied by the method of local adoptive transfer. Peritoneal cells from BCG-immunized mice transferred the reaction into unprimed recipients without any genetic restriction. In contrast, nonadherent peritoneal cells transferred the reaction only in H-2-compatible donor-recipient strain combinations. The use of H-2 recombinant strains has shown that delayed-type hypersensitivity to tuberculin in mice is restricted by the I-A locus. Monoclonal antibody against I-Ak beta abrogated (without complement) the transfer of the reaction to CBA recipients. The phenotype of nonadherent cells, transferring the reaction has been shown to be Thy-1+, Lyt-1+, 2-, L3T4+.
Nikonenko
B V
BV
Central Research Institute for Tuberculosis, Ministry of Public Health of the USSR, Moscow.
Mezhlumova
M B
MB
Apt
A S
AS
Moroz
A M
AM
D000818:Animals; D002874:Chromosome Mapping; D006968:Hypersensitivity, Delayed / Q000188:drug therapy / Q000235:genetics; D007116:Immunization, Passive; D051379:Mice; D008807:Mice, Inbred BALB C; D009163:Mycobacterium bovis / Q000032:analysis / Q000235:genetics; D010641:Phenotype; D014373:Tuberculin / Q000009:adverse effects*
D014373:Tuberculin