title: Isolation and characterization of ganglioside 9-O-acetyl-GD3 from bovine buttermilk.
abstract: Bovine buttermilk contains a unique ganglioside, 9-O-acetyl-GD3. In order to isolate large quantities of this ganglioside, a simplified isolation scheme which consists of several ion-exchange and silica gel column chromatographic procedures was devised. The isolated 9-O-acetyl-GD3 was characterized on the basis of its thin-layer chromatographic behavior, its immunoreactivity with a specific monoclonal antibody, JONES, and by conversion to authentic GD3 by mild base treatment.
authors.affiliation: Department of Biology, Yale University, New Haven, CT 06511.
authors.forename: D M
authors.initials: DM
authors.lastname: Bonafede
authors.forename: L J
authors.initials: LJ
authors.lastname: Macala
authors.forename: M
authors.initials: M
authors.lastname: Constantine-Paton
authors.forename: R K
authors.initials: RK
authors.lastname: Yu
mesh_terms: D064753:Ammonium Hydroxide; D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D005732:Gangliosides / Q000302:isolation & purification*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006878:Hydroxides; D007124:Immunoenzyme Techniques; D008545:Melanoma / Q000032:analysis; D008892:Milk / Q000032:analysis*
chemical_list: D005732:Gangliosides; D006878:Hydroxides; D064753:Ammonium Hydroxide; C049132:9-O-acetyl-GD3 ganglioside