title: The guanosine binding site of the Tetrahymena ribozyme.
abstract: The self-splicing Group I introns have a highly specific binding site for the substrate guanosine. Mutant versions of the Tetrahymena ribozyme have been used in combination with guanosine analogues to identify the nucleotide in the ribozyme that is primarily responsible for recognition of the guanine base.
authors.affiliation: Department of Molecular Biology, Massachusetts General Hospital, Boston 02114.
authors.forename: F
authors.initials: F
authors.lastname: Michel
authors.forename: M
authors.initials: M
authors.lastname: Hanna
authors.forename: R
authors.initials: R
authors.lastname: Green
authors.forename: D P
authors.initials: DP
authors.lastname: Bartel
authors.forename: J W
authors.initials: JW
authors.lastname: Szostak
mesh_terms: D000818:Animals; D001120:Arginine / Q000378:metabolism; D001483:Base Sequence; D002956:Citrulline / Q000378:metabolism; D005091:Exons; D006151:Guanosine / Q000378:metabolism*; D007438:Introns; D008969:Molecular Sequence Data; D009154:Mutation; D012322:RNA Precursors / Q000235:genetics; D012326:RNA Splicing; D016337:RNA, Catalytic; D012335:RNA, Ribosomal / Q000235:genetics* / Q000378:metabolism; D013768:Tetrahymena / Q000235:genetics*
chemical_list: D012322:RNA Precursors; D016337:RNA, Catalytic; D012335:RNA, Ribosomal; D006151:Guanosine; D002956:Citrulline; D001120:Arginine