[Immunohistochemical studies of insulin receptors in the liver].
The indirect peroxidase-labeled antibody method was used to localize Insulin Receptors (IR) in rat livers. For experiments the rabbit antisera against synthetic peptides correspond to the deduced amino acid sequence of alpha- and beta-subunits of human placenta insulin receptors were used as the first sera. Our results have shown that 30 min after intraperitoneal and intraventricular injections of I U insulin in controls and MSG-treated animals the staining of DAB reaction products in livers became stronger as compared to those injected with saline solution. After intraperitoneal and intraventricular injections of insulin the electron-microscopic investigations of rat livers have found the internalization of IR in hepatocytes by interaction of receptors with exo- and endogenous insulin.
Babichenko
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D000818:Animals; D002552:Cerebral Ventricles; D007124:Immunoenzyme Techniques; D007267:Injections; D007274:Injections, Intraperitoneal; D007328:Insulin / Q000008:administration & dosage; D008099:Liver / Q000032:analysis*; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D011972:Receptor, Insulin / Q000032:analysis*
D007328:Insulin; D011972:Receptor, Insulin
Immunogistokhimicheskoe issledovanie insulinovykh retseptorov pecheni.