title: [Use of an immunoperoxidase method in situ for detecting recombinant clones of vaccinia virus containing an expressed gene of influenza viral hemagglutinins].
abstract: The vaccine L-IVP strain of vaccinia virus (VV) was used to construct the recombinant viral clones containing the influenza A hemagglutinin gene. The recombinant T plasmid was obtained with HA gene inserted in the vector pGS-20 (B. Moss) under the 7.5 K promoter of VV. A homologous recombination technique was used to insert the gene with the flanking TK sequences into vaccinia virus genome. The recombinant clones were selected by dot-hybridization with [32P]-labeled HA-probe. These recombinants were analysed for HA gene expression by the indirect immunoperoxidase method in situ using the peroxidase conjugate of the staphylococcal A-protein. This technique allows to obtain stable stained preparation and analyse the protein behavior at the ultrastructural level.
authors.forename: T K
authors.initials: TK
authors.lastname: Selivanova
authors.forename: S V
authors.initials: SV
authors.lastname: Leonov
authors.forename: F P
authors.initials: FP
authors.lastname: Filatov
authors.forename: V P
authors.initials: VP
authors.lastname: Iuferov
authors.forename: M M
authors.initials: MM
authors.lastname: Garaev
authors.forename: A F
authors.initials: AF
authors.lastname: Bobkov
authors.forename: Iu Iu
authors.initials: IuIu
authors.lastname: Rassulin
authors.forename: T M
authors.initials: TM
authors.lastname: Khizhniakova
authors.forename: L V
authors.initials: LV
authors.lastname: Uryvaev
authors.forename: I G
authors.initials: IG
authors.lastname: Kharitonenkov
mesh_terms: D002999:Clone Cells; D004279:DNA, Viral / Q000235:genetics; D015870:Gene Expression; D005814:Genes, Viral; D006389:Hemagglutinins, Viral / Q000235:genetics*; D007124:Immunoenzyme Techniques; D009980:Influenza A virus / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D013205:Staphylococcal Protein A; D014616:Vaccinia virus / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D006389:Hemagglutinins, Viral; D013205:Staphylococcal Protein A
vernacular_title: Ispol'zovanie immunoperoksidaznogo metoda in situ dlia vyiavleniia rekombinantnykh klonov virusa ospovaktsiny, soderzhashchikh ékspressiruemyĭ gen gemaggliutinina virusa grippa.