title: Recent trends in platelet antigen/antibody detection.
abstract: The detection of platelet antigens and platelet antibodies has always been difficult. Recent technical achievements are due to the availability of more specific and sensitive reagents (i.e. F(ab)2 fragments; higher specific activity of labels; monoclonal antibodies directed against platelet membrane constituents etc.) and the application of advanced immunological assays to platelet immunology (i.e. blotting assays, "capture"-ELISA's and radioimmunoprecipitation in conjunction with SDS polyacrylamide gel electrophoresis). These techniques have permitted the definition and immuno-chemical characterization of new platelet allo- and autoantigens, have assisted in clinical diagnosis and promoted our understanding of pathogenic mechanisms. Illustrative examples are presented and discussed.
authors.affiliation: Institute for Clinical Immunology and Transfusion Medicine, Justus-Liebig-University, Giessen, Federal Republic of Germany.
authors.forename: C
authors.initials: C
authors.lastname: Mueller-Eckhardt
authors.forename: V
authors.initials: V
authors.lastname: Kiefel
authors.forename: S
authors.initials: S
authors.lastname: Santoso
mesh_terms: D000906:Antibodies / Q000032:analysis*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000937:Antigen-Antibody Reactions; D001792:Blood Platelets / Q000276:immunology*; D003168:Complement Fixation Tests; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D007158:Immunologic Techniques; D010980:Platelet Membrane Glycoproteins / Q000276:immunology; D011233:Precipitin Tests
chemical_list: D000906:Antibodies; D000911:Antibodies, Monoclonal; D010980:Platelet Membrane Glycoproteins