Biosynthesis of titin in cultured skeletal muscle cells.
Although significant progress has been made regarding the structure and function of titin, little data exist on the biosynthesis of this large protein in developing muscle. Using pulse-labeling with [35S]methionine and immunoprecipitation with an anti-titin mAb, we have examined the biosynthesis of titin in synchronized cultures of skeletal muscle cells derived from day 12 chicken embryos. We find that: (a) titin synthesis increases greater than 4-fold during the first week in culture and during this same time period, synthesis of muscle-specific myosin heavy chain increases greater than 12-fold; (b) newly synthesized titin has a t1/2 of approximately 70 h; (c) titin is resistant to extraction with Triton X-100 both during and immediately after its synthesis. These observations suggest that newly synthesized titin molecules are stable proteins that rapidly associate with the cytoskeleton of developing myotubes.
Isaacs
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WB
Department of Biochemistry, University of Iowa, Iowa City 52242.
Kim
I S
IS
Struve
A
A
Fulton
A B
AB
D000818:Animals; D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D002645:Chickens; D064211:Connectin; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D007700:Kinetics; D008565:Membrane Proteins / Q000096:biosynthesis*; D008715:Methionine / Q000378:metabolism; D009124:Muscle Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D009132:Muscles / Q000378:metabolism*; D011494:Protein Kinases; D013462:Sulfur Radioisotopes
D000911:Antibodies, Monoclonal; D064211:Connectin; D008565:Membrane Proteins; D009124:Muscle Proteins; D013462:Sulfur Radioisotopes; D008715:Methionine; D011494:Protein Kinases