title: Structure of a Ruminococcus albus endo-1,4-beta-glucanase gene.
abstract: A chromosomal DNA fragment encoding an endo-1,4-beta-glucanase I (Eg I) gene from Ruminococcus albus cloned and expressed in Escherichia coli with pUC18 was fully sequenced by the dideoxy-chain termination method. The sequence contained a consensus promoter sequence and a structural amino acid sequence. The initial 43 amino acids of the protein were deduced to be a signal sequence, since they are missing in the mature protein (Eg I). High homology was found when the amino acid sequence of the Eg I was compared with that of endoglucanase E from Clostridium thermocellum. Codon usage of the gene was not biased. These results suggested that the properties of the Eg I gene from R. albus was specified from the known beta-glucanase genes of the other organisms.
authors.affiliation: Department of Food Science and Technology, School of Agriculture, Nagoya University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ohmiya
authors.forename: T
authors.initials: T
authors.lastname: Kajino
authors.forename: A
authors.initials: A
authors.lastname: Kato
authors.forename: S
authors.initials: S
authors.lastname: Shimizu
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002480:Cellulase / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010459:Peptococcaceae / Q000201:enzymology / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D002480:Cellulase