title: New mutations fts-36, lts-33, and ftsW clustered in the mra region of the Escherichia coli chromosome induce thermosensitive cell growth and division.
abstract: Three new mutants of Escherichia coli showing thermosensitive cell growth and division were isolated, and the mutations were mapped to the mra region at 2 min on the E. coli chromosome map distal to leuA. Two mutations were mapped closely upstream of ftsI (also called pbpB), in a region of 600 bases; the fts-36 mutant showed thermosensitive growth and formed filamentous cells at 42 degrees C, whereas the lts-33 mutant lysed at 42 degrees C without forming filamentous cells. The mutation in the third new thermosensitive, filament-forming mutant, named ftsW, was mapped between murF and murG. By isolation of these three mutants, about 90% of the 17-kilobase region from fts-36-lts-33 to envA could be filled with genes for cell division and growth, and the genes could be aligned.
authors.affiliation: Institute of Applied Microbiology, University of Tokyo, Japan.
authors.forename: F
authors.initials: F
authors.lastname: Ishino
authors.forename: H K
authors.initials: HK
authors.lastname: Jung
authors.forename: M
authors.initials: M
authors.lastname: Ikeda
authors.forename: M
authors.initials: M
authors.lastname: Doi
authors.forename: M
authors.initials: M
authors.lastname: Wachi
authors.forename: M
authors.initials: M
authors.lastname: Matsuhashi
mesh_terms: D001483:Base Sequence; D002455:Cell Division; D004252:DNA Mutational Analysis; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D006358:Hot Temperature; D010957:Plasmids; D015183:Restriction Mapping