title: Bombesin stimulation of inositol 1,4,5-trisphosphate generation and intracellular calcium release is amplified in a cell line overexpressing the N-ras proto-oncogene.
abstract: Bombesin stimulation of T15 cells in which the inducible N-ras oncogene is overexpressed caused elevated production of inositol phosphates compared to uninduced cells [Wakelam, Davies, Houslay, McKay, Marshall & Hall (1986) Nature (London) 323, 173-176]. This elevated response is shown here to result from increased generation of inositol 1,4,5-trisphosphate leading to an elevated release of intracellular stored Ca2+. Single-cell analysis of Ca2+ release showed that the elevated response is not a consequence of an increased fraction of responding cells. These amplifications are consistent with p21N-ras acting like a guanine nucleotide coupling protein in this cell line.
authors.affiliation: Institute of Cancer Research, Chester Beatty Laboratories, London, U.K.
authors.forename: A C
authors.initials: AC
authors.lastname: Lloyd
authors.forename: S A
authors.initials: SA
authors.lastname: Davies
authors.forename: I
authors.initials: I
authors.lastname: Crossley
authors.forename: M
authors.initials: M
authors.lastname: Whitaker
authors.forename: M D
authors.initials: MD
authors.lastname: Houslay
authors.forename: A
authors.initials: A
authors.lastname: Hall
authors.forename: C J
authors.initials: CJ
authors.lastname: Marshall
authors.forename: M J
authors.initials: MJ
authors.lastname: Wakelam
mesh_terms: D001839:Bombesin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D005784:Gene Amplification; D011905:Genes, ras; D015544:Inositol 1,4,5-Trisphosphate; D007295:Inositol Phosphates / Q000378:metabolism*; D013403:Sugar Phosphates / Q000378:metabolism*
chemical_list: D007295:Inositol Phosphates; D013403:Sugar Phosphates; D015544:Inositol 1,4,5-Trisphosphate; D001839:Bombesin; D002118:Calcium