Multiple modifications in the phosphoproteins bound to stored messenger RNA in Xenopus oocytes.
Messenger RNA molecules accumulated in amphibian oocytes are stabilized and blocked from translation through association with a defined set of phosphoproteins. Phosphoproteins of 60 kDa and 56 kDa (pp60 and pp56) isolated from messenger ribonucleoprotein particles of Xenopus laevis oocytes can be bound in vitro to mRNA sequences. After phospholabelling in vitro, both pp60 and pp56 show a range of ionic forms, which resolve on two-dimensional gel electrophoresis as a series of pairs with identical charge. The similarities between pp60 and pp56 in their ionic properties suggest a common protein primary structure. This suggestion gains further support from proteinase digestion analysis of pp60 and pp56: practically identical size patterns of phospholabelled fragments are generated using a range of different proteinases. However, in spite of their structural similarities, pp60 and pp56 are recognised as antigenically distinct from each other by using polyclonal antibodies. It is concluded from these, and other, observations that pp60 and pp56 are members of a family of structurally similar polypeptides which are subjected to multiple secondary modifications. Of these modifications, phosphorylation appears to be instrumental in establishing tight binding to mRNA, while antigenicity appears to be determined by some other modification. The role of microheterogeneity in the structure of RNA-binding proteins is discussed in relation to the differential activation of mRNA sequences for translation during early development.
Cummings
A
A
Department of Biology, University of St. Andrews, U.K.
Barrett
P
P
Sommerville
J
J
D000818:Animals; D000911:Antibodies, Monoclonal; D001345:Autoradiography; D002352:Carrier Proteins / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D010450:Endopeptidases; D015151:Immunoblotting; D029543:Nucleocytoplasmic Transport Proteins; D009865:Oocytes / Q000378:metabolism*; D010446:Peptide Fragments / Q000032:analysis; D010750:Phosphoproteins / Q000276:immunology / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins / Q000276:immunology / Q000378:metabolism; D014982:Xenopus laevis
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D029543:Nucleocytoplasmic Transport Proteins; D010446:Peptide Fragments; D010750:Phosphoproteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; C026452:messenger ribonucleoprotein; D010450:Endopeptidases