Expression of myc and ras oncogenes in two newly established neuroblastoma cell lines.
Two new neuroblastoma cell lines, KG-MH and KM-YH have been established from fresh tumour samples. In vitro growth characteristics are presented together with a karyological analysis. Northern and Southern blot experiments have been performed using molecularly cloned probes for c-myc, N-myc, c-Ha-ras, c-Ki-ras, and N-ras oncogenes. Both cell lines showed expression for N-myc, while c-myc expression was not detected. Cell line KM-YH, with a rather long population doubling time of 78 h, showed additional expression for the three ras genes.
Csaikl
F
F
Institute of Tumorbiology/Cancer Research, University of Vienna, Austria.
Müllauer
L
L
Csaikl
U
U
Vetterlein
M
M
D005784:Gene Amplification; D011905:Genes, ras; D006801:Humans; D009447:Neuroblastoma / Q000235:genetics*; D009857:Oncogenes; D014407:Tumor Cells, Cultured