Demonstration of interleukin-1 beta on perifollicular endothelial cells in the thyroid glands of patients with Graves' disease.
We examined the presence of interleukin-1 (IL-1) in the thyroid glands of 20 patients with Graves' disease and 10 control subjects, using anti-IL-1 monoclonal antibodies in conjunction with immunohistochemical techniques. In 13 (65%) Graves' disease patients, IL-1 beta, but not IL-1 alpha, was demonstrated on the capillary endothelial cells (EC) around follicles (perifollicular EC). However, the EC of arteries and veins were negative for both IL-1 beta and IL-1 alpha. In the control group, none of the thyroid glands showed positive staining for IL-1 beta or IL-1 alpha. There was a significant correlation (P less than 0.025) between the presence of IL-1 beta on perifollicular EC and the presence of serum antimicrosomal antibodies. These results provide an important insight into the involvement of IL-1 beta in the development of Graves' disease.
Miyazaki
A
A
Second Department of Internal Medicine, Osaka University Medical School, Japan.
Hanafusa
T
T
Itoh
N
N
Miyagawa
J
J
Kono
N
N
Tarui
S
S
Kiyotaki
C
C
Yoshizaki
K
K
D000293:Adolescent; D000328:Adult; D000911:Antibodies, Monoclonal; D001158:Arteries / Q000276:immunology; D002196:Capillaries / Q000276:immunology; D002648:Child; D004730:Endothelium, Vascular / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique; D006111:Graves Disease / Q000276:immunology* / Q000473:pathology; D006680:HLA Antigens / Q000032:analysis; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis*; D008297:Male; D012016:Reference Values; D013961:Thyroid Gland / Q000098:blood supply* / Q000473:pathology; D014680:Veins / Q000276:immunology
D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D007375:Interleukin-1