The mouse type IV c-abl gene product is a nuclear protein, and activation of transforming ability is associated with cytoplasmic localization.
The subcellular localization of the mouse type IV c-abl protein was determined by indirect immunofluorescence of nontransformed NIH 3T3 fibroblasts that overexpress the protein. Unlike the viral transforming protein p160gag/v-abl, which has cytoplasmic and plasma membrane localization, a large fraction of the c-abl (IV) protein is nuclear, with the remainder in the cytoplasm and plasma membrane. Deletion of a small N-terminal regulatory region of the c-abl (IV) protein, sufficient to activate its transforming potential fully, changes the distribution of the protein from the nucleus to the cytoplasm. Mapping of an amino acid sequence responsible for the nuclear localization of the c-abl (IV) protein reveals a nuclear localization signal similar to that of SV40 large T antigen.
Van Etten
R A
RA
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142.
Jackson
P
P
Baltimore
D
D
D000595:Amino Acid Sequence; D000818:Animals; D002451:Cell Compartmentation; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004252:DNA Mutational Analysis; D005455:Fluorescent Antibody Technique; D051379:Mice; D008969:Molecular Sequence Data; D019814:Myristic Acid; D009227:Myristic Acids / Q000502:physiology; D009687:Nuclear Proteins / Q000502:physiology* / Q000648:ultrastructure; D011499:Protein Processing, Post-Translational; D011518:Proto-Oncogene Proteins / Q000502:physiology* / Q000648:ultrastructure; D016315:Proto-Oncogene Proteins c-abl
D009227:Myristic Acids; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; D019814:Myristic Acid; D016315:Proto-Oncogene Proteins c-abl