The isolation of B lymphocytes from human peripheral blood using antibodies coupled to paramagnetic particles and rosetting techniques.
A method is described for the complete removal of monocytes and a reduction of natural killer cells from a suspension of peripheral blood mononuclear leukocytes. The cells are depleted in a batch procedure employing monoclonal antibodies coupled to paramagnetic particles. The bound cells are removed with the help of a magnet. The resulting cell population, which contains less than 1% esterase positive cells and a fraction of the natural killer cells originally present, can be further depleted of T lymphocytes by erythrocyte-rosetting techniques.
Hebell
T
T
Department of Immunology, Georg August Universität Göttingen, F.R.G.
Götze
O
O
D000911:Antibodies, Monoclonal / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology*; D002469:Cell Separation / Q000379:methods; D002470:Cell Survival; D006801:Humans; D007158:Immunologic Techniques; D066298:In Vitro Techniques; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology; D008280:Magnetics; D009000:Monocytes / Q000166:cytology / Q000276:immunology; D012397:Rosette Formation
D000911:Antibodies, Monoclonal