An altered v-raf is required in addition to v-myc in J3V1 virus for acceleration of murine plasmacytomagenesis.
We have isolated and molecularly cloned a highly pathogenic virus variant, J3V1, from murine plasmacytomas induced by a combination of pristane and the weakly transforming recombinant retrovirus J3. J3 virus is a derivative of the v-raf/v-myc-carrying J2 virus that was generated by a frameshifting deletion inactivating v-raf in J2. J3V1 contains an additional deletion of 334 base paris in gag, which restores the correct reading frame for v-raf and results in the expression of a p57 gag-raf fusion protein. Reactivation of v-raf in J3 is required for efficient plasmacytoma acceleration in pristane-conditioned BALB/cAn mice.
Troppmair
J
J
National Cancer Institute, Frederick Cancer Research Facility, MD 21701.
Potter
M
M
Wax
J S
JS
Rapp
U R
UR
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002455:Cell Division; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D016274:Oncogene Protein p55(v-myc); D027384:Oncogene Proteins v-raf; D009857:Oncogenes; D010954:Plasmacytoma / Q000235:genetics*; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D015183:Restriction Mapping; D012190:Retroviridae / Q000235:genetics* / Q000472:pathogenicity; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics*
D004279:DNA, Viral; D016274:Oncogene Protein p55(v-myc); D015682:Retroviridae Proteins, Oncogenic; D011505:Protein-Tyrosine Kinases; D027384:Oncogene Proteins v-raf