Studies on bilokinase, a biliary plasminogen activator: immunologic property and organ distribution.
The aim of the present study was to elucidate the immunologic difference between bilokinase, a plasminogen activator in bile, and urokinase, an urinary plasminogen activator, and to demonstrate the organ distribution of these antigens. Polyclonal antibodies against the highly purified activators (bovine-bilokinase, -urokinase and human urokinase) were raised in rabbits or guinea pigs. Immunologic precipitin reactions and quenching were found to occur only between the activators and their respective antisera. Histo-immunologic demonstration of the activators revealed that bilokinase-antigen was localized mainly in the hepatocytes as well as in the mucosa of the gall bladder, whereas urokinase-antigen was present in the epithelium of the urinary tubules. The liver may have a potential role in the hepatobiliary fibrinolytic system.
Ariga
T
T
College of Agriculture and Veterinary Medicine, Nihon University, Tokyo, Japan.
Oshiba
S
S
Seki
T
T
Imai
H
H
Imai
S
S
Sawai
H
H
D000818:Animals; D002417:Cattle; D010450:Endopeptidases / Q000032:analysis / Q000276:immunology* / Q000502:physiology; D006168:Guinea Pigs; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D011233:Precipitin Tests; D011817:Rabbits; D014018:Tissue Distribution; D014568:Urokinase-Type Plasminogen Activator / Q000276:immunology
D007106:Immune Sera; D010450:Endopeptidases; D014568:Urokinase-Type Plasminogen Activator; C019790:bilokinase