title: Immunofluorescence colocalization of the 90-kDa heat-shock protein and microtubules in interphase and mitotic mammalian cells.
abstract: A mouse monoclonal antibody (AC88) that was raised against the 88-kDa heat-shock protein of the water mold, Achlya ambisexualis, and that cross-reacts with the 90-kDa mammalian heat-shock protein (hsp90), and an antibody against tubulin were used to localize hsp90 and microtubules, respectively, in the same cultured rat endothelial and PtK1 epithelial cells by indirect immunofluorescence. AC88 and tubulin antibodies labeled the same structures in cells at all stages of the cell cycle, regardless of whether cells were permeabilized before or after fixation. Labeling of cell structures by both AC88 and anti-tubulin antibodies was identically affected by treating cells with colcemid. Double labeling with AC88 and anti-tubulin antibodies in interphase and mitotic cells is consistent with the conclusion that all microtubules are labeled and that no subclass of microtubules is preferentially labeled. Fluorescent labeling by AC88 was prevented by preabsorption of the antibody with purified rat hsp90 but was unaffected by preabsorption with purified 6S tubulin dimer. In contrast to AC88, fluorescent labeling by an anti-tubulin antibody was prevented by preabsorption with tubulin dimer but was unaffected by preabsorption with rat hsp90. Western-blot analysis demonstrated no cross-reactivity of AC88 for tubulin and no cross-reactivity of the anti-tubulin antibody for hsp90. A polyclonal antiserum fraction from a rabbit immunized with the 89-kDa heat-shock protein from chicken also labeled the mitotic apparatus in dividing cells and, somewhat less distinctly, fibrous structures in interphase cells. Labeling by hsp89 anti-serum was prevented by absorption with hsp90. AC88 also labeled microtubules in cultured mouse (L929 and 3T3), rat (endothelium and TRST), hamster (CHO) and primate (BSC, COS-1 and HeLa) cell lines. The demonstration of colocalization of hsp90 with microtubules should provide a valuable clue to eventual understanding of the cellular function of this ubiquitous, conserved and abundant stress-response protein.
authors.affiliation: Department of Anatomy and Cell Biology, University of Michigan Medical School, Ann Arbor 48109-0616.
authors.forename: T
authors.initials: T
authors.lastname: Redmond
authors.forename: E R
authors.initials: ER
authors.lastname: Sanchez
authors.forename: E H
authors.initials: EH
authors.lastname: Bresnick
authors.forename: M J
authors.initials: MJ
authors.lastname: Schlesinger
authors.forename: D O
authors.initials: DO
authors.lastname: Toft
authors.forename: W B
authors.initials: WB
authors.lastname: Pratt
authors.forename: M J
authors.initials: MJ
authors.lastname: Welsh
mesh_terms: D000818:Animals; D000906:Antibodies; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D006360:Heat-Shock Proteins / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D007399:Interphase; D008870:Microtubules / Q000032:analysis*; D008938:Mitosis; D011233:Precipitin Tests; D008941:Spindle Apparatus / Q000032:analysis*; D014404:Tubulin / Q000032:analysis / Q000276:immunology
chemical_list: D000906:Antibodies; D006360:Heat-Shock Proteins; D014404:Tubulin