Detection and characterization of endothelin-1-like immunoreactivity in rat plasma.
Using two radioimmunoassays (RIAs) for endothelin-1 (ET-1) with and without a substantial cross-reactivity with ET-3, we have measured the plasma ET-1-like immunoreactivity (-LI) level in rat plasma. ET-1-LI was detected in plasma from male Wistar rats. ET-1-LI in rat plasma consisted of three components with molecular weights of 6K, 4K and 2.5K daltons by gel permeation chromatography. Two of the components were eluted at positions of big ET (4K) and synthetic ET-1 (2.5K). The remaining component was eluted at the preceding fraction (6K). No difference was observed in ET-1-LI of the small molecular form of ET (2.5K) between the two RIAs. Thus, there is little or no ET-3 in rat plasma, which has the sequence found originally in the rat genome. The concentration of the small molecular form of ET, presumably ET-1, in rat plasma was about 4 pg/ml.
Saito
Y
Y
Department of Medicine, Kyoto University School of Medicine, Japan.
Nakao
K
K
Shirakami
G
G
Jougasaki
M
M
Yamada
T
T
Itoh
H
H
Mukoyama
M
M
Arai
H
H
Hosoda
K
K
Suga
S
S
D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D002850:Chromatography, Gel; D003429:Cross Reactions; D016232:Endothelins; D004730:Endothelium, Vascular / Q000502:physiology; D008297:Male; D010455:Peptides / Q000097:blood* / Q000302:isolation & purification; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains
D000906:Antibodies; D000911:Antibodies, Monoclonal; D016232:Endothelins; D010455:Peptides