DNA-damaging agents show different kinetics in induction of heterothallic mating-type switching during growth after treatment in yeast.
Heterothallic mating-type switching in Saccharomyces cerevisiae has been shown to be inducible by DNA-damaging agents (Schiestl and Wintersberger, 1983). Different DNA-damaging agents differ greatly in their kinetics of induction during incubation after treatment. Irradiation with X-rays resulted in an increase in the frequency immediately after exposure and no further increase was seen during incubation after treatment. Nitrous acid and 4-nitroquinoline-N-oxide, on the other hand, did not show any increase in frequency immediately after treatment, but require post-treatment incubation to produce an increased frequency of heterothallic mating-type switching. UV irradiation and ethyl methanesulfonate result in induction to certain levels immediately after treatment, but further induction was seen during post-treatment incubation. The results may indicate that certain kinds of DNA damage require repair of replication to be converted into recombinogenic lesions.
Schiestl
R H
RH
Department of Molecular Genetics, University of Vienna, Austria.
D004249:DNA Damage; D004271:DNA, Fungal / Q000187:drug effects / Q000528:radiation effects; D005800:Genes, Fungal; D049770:Genes, Mating Type, Fungal; D009608:Nitrous Acid / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013997:Time Factors; D014466:Ultraviolet Rays; D014965:X-Rays
D004271:DNA, Fungal; D009608:Nitrous Acid