[Flow cytometric analysis of oncogene products].
Flow cytometry (FCM) of oncogene products which opens new avenues of cell biological investigation of human neoplasia is being reviewed. Using H-ras p21/DNA dual FCM, patients with DNA-aneuploid multiple myeloma (MM) were examined. The patients whose MM cells expressed high level of H-ras p21 had poor prognosis. Specificity of this assay was appraised extensively. It is not likely that H-ras p21 expressed in MM is of oncogenic form since point mutation of H-ras gene was not reported in B cell chronic lymphocytic leukemia which is closely located to MM in B lymphocyte differentiation lineage. High expression of H-ras p21 in MM seems to be related to cell proliferation and/or differentiation. H-ras p21/DNA dual FCM is applicable to analyse the pathophysiology of tumor cells. FCM analyses of other oncogene products and proteins related to cell proliferation, c-myc, p53 and Ki-67, were also described. Multiparameter FCM analysis is quite suited to examine expression of these proteins in situ.
Tsuchiya
H
H
Dept. of Pediatrics, Kumamoto University Medical School.
D004273:DNA, Neoplasm / Q000235:genetics; D005434:Flow Cytometry; D006801:Humans; D009101:Multiple Myeloma / Q000235:genetics; D009369:Neoplasms / Q000032:analysis / Q000235:genetics*; D015689:Oncogene Protein p21(ras); D016274:Oncogene Protein p55(v-myc); D009856:Oncogene Proteins, Viral / Q000032:analysis; D011518:Proto-Oncogene Proteins / Q000032:analysis*; D012191:Retroviridae Proteins / Q000032:analysis
D004273:DNA, Neoplasm; D016274:Oncogene Protein p55(v-myc); D009856:Oncogene Proteins, Viral; D011518:Proto-Oncogene Proteins; D012191:Retroviridae Proteins; D015689:Oncogene Protein p21(ras)