title: Gastrin-releasing peptide-like immunoreactivity in porcine follicular fluid and ovary.
abstract: Porcine follicular fluid was found to contain gastrin-releasing peptide (GRP) by radioimmunoassay. High pressure liquid chromatography of the extracted material showed two peaks of GRP immunoreactivity that closely resembled the C-terminal fragments of GRP, GRP18-27 and GRP14-27. Immunohistochemical studies revealed specific staining for GRP in the granulosa cells of adult porcine ovary. These results demonstrate the presence of substances which behave like authentic GRP-like peptides in porcine ovary and follicular fluid and suggest that these peptides may play a paracrine and/or autocrine role in the regulation of the ovarian function.
authors.affiliation: V Medical Clinic, Policlinico Umberto I, University of Rome La Sapienza, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Gnessi
authors.forename: S
authors.initials: S
authors.lastname: Altamura
authors.forename: M
authors.initials: M
authors.lastname: Arizzi
authors.forename: C
authors.initials: C
authors.lastname: Tei
authors.forename: C
authors.initials: C
authors.lastname: La Torre
authors.forename: V
authors.initials: V
authors.lastname: Bonifacio
authors.forename: F
authors.initials: F
authors.lastname: Facchinetti
authors.forename: G
authors.initials: G
authors.lastname: Spera
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005260:Female; D015571:Follicular Fluid / Q000032:analysis*; D019886:Gastrin-Releasing Peptide; D007124:Immunoenzyme Techniques; D010053:Ovary / Q000032:analysis*; D010455:Peptides / Q000032:analysis*; D011863:Radioimmunoassay; D013552:Swine
chemical_list: D010455:Peptides; D019886:Gastrin-Releasing Peptide