A mutation in the dam gene of Vibrio cholerae: 2-aminopurine sensitivity with intact GATC methylase activity.
Vibrio cholerae mutants sensitive to 2-aminopurine (2AP) but with DNA adenine methylase activity similar to parental cells have been isolated. The mutant strains were sensitive to ultraviolet light (UV), methyl methane sulphonate (MMS) and 9-aminoacridine. The spontaneous mutation frequency of the mutants were not significantly affected. Attempts to isolate dam V. cholerae cells by screening 2AP sensitive cells have not been successful. All the mutant phenotypes could be suppressed by introducing the plasmid pRB103 carrying the dam gene of Escherichia coli into the mutant cells.
Bandyopadhyay
R
R
Biophysics Division, Indian Institute of Chemical Biology, Calcutta.
Sengupta
A
A
Das
J
J
D015075:2-Aminopurine / Q000494:pharmacology*; D000225:Adenine / Q000031:analogs & derivatives*; D000585:Aminacrine / Q000494:pharmacology; D001435:Bacteriophages / Q000235:genetics; D001483:Base Sequence; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D007700:Kinetics; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D009154:Mutation; D010957:Plasmids; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000235:genetics*; D013379:Substrate Specificity; D014466:Ultraviolet Rays; D014734:Vibrio cholerae / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects
D015075:2-Aminopurine; D000585:Aminacrine; D008741:Methyl Methanesulfonate; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); D000225:Adenine