Purification of labelled antibodies by hydrophobic interaction chromatography.
In order to improve the sensitivity of immunometric assays, a chromatographic technique was developed that virtually climinates components causing non-specific background. Labelled antibodies were applied to a phenyl-Sepharose column in physiological buffer. When labelled antibodies were purified by this technique, the non-specific background of various time-resolved immunofluorometric assays was reduced 3- to 10-fold and was very close to the instrument background. The assay sensitivity was simultaneously increased by a factor of 2 to 16. This purification method might be used to improve the results of immunometric assays in general.
Alfthan
H
H
Department I of Obstetrics and Gynecology, Helsinki University Central Hospital, Finland.
Stenman
U H
UH
D000906:Antibodies / Q000276:immunology / Q000302:isolation & purification*; D006063:Chorionic Gonadotropin / Q000276:immunology; D002853:Chromatography, Liquid / Q000379:methods; D005455:Fluorescent Antibody Technique
D000906:Antibodies; D006063:Chorionic Gonadotropin