Isolation and sequence determination of rat islet amyloid polypeptide.
Rat islet amyloid polypeptide (IAPP) was isolated from the pancreata of normal rats by utilizing cross-reactivity of a radioimmunoassay system for human IAPP with rat IAPP. Rat IAPP was a 37-amino acid polypeptide with tyrosine amide at the C-terminus, as was the case with human IAPP. Amino acid sequences of rat and human IAPPs were 84% identical, and the most highly conserved sequences were found in the N- and C-terminal regions. Rat IAPP sequence was also 51% identical to those of alpha and beta rat calcitonin gene-related peptide sequences.
Asai
J
J
Third Department of Internal Medicine, Miyazaki Medical College, Japan.
Nakazato
M
M
Kangawa
K
K
Matsukura
S
S
Matsuo
H
H
D000595:Amino Acid Sequence; D000682:Amyloid / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D058228:Islet Amyloid Polypeptide; D007515:Islets of Langerhans / Q000032:analysis*; D008969:Molecular Sequence Data; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D012689:Sequence Homology, Nucleic Acid
D000682:Amyloid; D058228:Islet Amyloid Polypeptide