A problem encountered using staphylococcus/streptococcus supplement.
A problem was encountered while using a commercial staphylococcus/streptococcus selective supplement in blood agar culture medium. A number of strains of Staphylococcus aureus failed to grow, although they grew well on non-selective media. The phenomenon was shown to be a result of a pH change caused by incubation in a carbon dioxide-enriched atmosphere, potentiating the activity of the nalidixic acid component. It is recommended that media containing this supplement are not incubated in CO2 enriched atmospheres.
Morton
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CE
Holt
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HA
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001431:Bacteriological Techniques; D003470:Culture Media; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development*
D000900:Anti-Bacterial Agents; D003470:Culture Media