Preparation of monoclonal antibodies against nuclear antigens in a DNA affinity purified protein fraction from chick embryo extract.
Monoclonal antibodies (Mabs) were raised to components of a DNA binding protein fraction obtained by using native and denatured DNA-cellulose column chromatographies of chick embryo extract. Indirect immunofluorescence microscopic analysis revealed that 3 Mabs, Pr-28, Pr-123 (or Pr-122) and Pr-192 react to nuclear antigens of proliferating cells. Part of the cell nuclei in chick embryo fibroblast culture were stained in speckled patterns by all three of the Mabs. It seems however that they are different clones judging from the following criteria; 1. Pr-28 crossreacts to a P3U1 cell surface antigen but Pr-123 (or Pr-122) and Pr-192 do not. 2. The rate of nuclei stained by Pr-122 is different from that of Pr-192 in both growing and quiescent cultures.
Matsuhashi
S
S
Department of Biochemistry, Saga Medical School, Japan.
Arai
Y
Y
Watanabe
T
T
Hori
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000145:classification / Q000276:immunology; D000918:Antibody Specificity; D000954:Antigens, Surface / Q000276:immunology; D002455:Cell Division; D002467:Cell Nucleus / Q000276:immunology; D002642:Chick Embryo; D002846:Chromatography, Affinity; D004247:DNA / Q000302:isolation & purification / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000276:immunology* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000166:cytology / Q000276:immunology; D005455:Fluorescent Antibody Technique; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D014020:Tissue Extracts
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D004268:DNA-Binding Proteins; D014020:Tissue Extracts; D004247:DNA