Bacterial endotoxin and tumor necrosis factor stimulate prostaglandin production by human decidua.
The purpose of these studies was to determine the effect of bacterial endotoxin and tumor necrosis factor (TNF) on prostaglandin (PG) secretion by human decidua. Decidual explants were established from women undergoing elective cesarean sections before the onset of labor. Escherichia Coli endotoxin and purified human recombinant TNF (rh TNF) were incubated with decidual explants. PGF2 alpha and PGE2 biosynthesis was measured by radioimmunoassay. A significant increase in the release of all PGs into the media occurred in response to LPS and TNF. In the setting of an extraamniotic infection, bacterial and host secretory products (TNF) could trigger the onset of labor, activating the decidua to produce PGs.
Romero
R
R
Department of Obstetrics and Gynecology, Yale University School of Medicine, New Haven, Connecticut 06510.
Mazor
M
M
Wu
Y K
YK
Avila
C
C
Oyarzun
E
E
Mitchell
M D
MD
D003656:Decidua / Q000187:drug effects / Q000378:metabolism*; D015237:Dinoprost / Q000096:biosynthesis* / Q000378:metabolism; D015232:Dinoprostone / Q000096:biosynthesis* / Q000378:metabolism; D004731:Endotoxins / Q000494:pharmacology*; D004926:Escherichia coli; D005260:Female; D006801:Humans; D011247:Pregnancy; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D004731:Endotoxins; D014409:Tumor Necrosis Factor-alpha; D015237:Dinoprost; D015232:Dinoprostone