Phenotypical study of human lymphokine-activated killer (LAK) cells.
In the present study, T-cell enriched lymphocyte populations were stimulated with recombinant interleukin-2 (IL-2) to obtain lymphokine-activated killer (LAK) cells, and their cytotoxicity was tested against different target cells. The expression of phenotypical markers with a panel of monoclonal antibodies (Moabs) using indirect immunofluorescence techniques and cytofluorometric analysis was studied. The results showed slight variations in T-cell antigen expression and an increase in the expression of immature NK and proliferation associated antigens.
Barral
A M
AM
National Institute of Oncology and Radiobiology, Vedado, Cuba.
García
C A
CA
D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis*; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D005455:Fluorescent Antibody Technique; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology*; D010641:Phenotype; D011994:Recombinant Proteins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D007376:Interleukin-2; D011994:Recombinant Proteins