A transfected L-myc gene can substitute for c-myc in blocking murine erythroleukemia differentiation.
We investigated the ability of the proto-oncogene L-myc to substitute for c-myc in blocking murine erythroleukemia differentiation. Murine erythroleukemia cells (line C19) were transfected with recombinant plasmids containing genomic and cDNA fragments of the L-myc gene driven by a Moloney murine leukemia virus long terminal repeat. Clones expressing constitutive high levels of L-myc failed to differentiate in response to the chemical inducer N,N'-hexamethylene bisacetamide (HMBA). The block to differentiation correlated with the level of L-myc expression. Furthermore, transfected clones grown in the presence of inducer for an extended period of time showed an increased level of L-myc expression. These results suggest that functional domains of the c-myc gene involved in differentiation are located in the discrete regions of homology between the c- and L-myc genes.
Birrer
M J
MJ
Navy Medical Oncology Branch, National Cancer Institute, Bethesda, Maryland 20892.
Raveh
L
L
Dosaka
H
H
Segal
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D000081:Acetamides; D015152:Blotting, Northern; D002454:Cell Differentiation; D002461:Cell Line, Transformed; D004247:DNA; D005914:Globins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D008979:Moloney murine leukemia virus / Q000235:genetics; D010957:Plasmids; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
D000081:Acetamides; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D005914:Globins; D004247:DNA; C014026:hexamethylene bisacetamide