title: The suppressor gene scl1+ of Saccharomyces cerevisiae is essential for growth.
abstract: In Saccharomyces cerevisiae, the SCL-1 mutation is a dominant suppressor of the cycloheximide-resistant, temperature-sensitive (ts) lethal mutation, crl3 [McCusker and Haber, Genetics 119 (1988a) 303-315]. The wild-type scl1+ gene was isolated by screening subclones of the 35-kb region between TRP5 and LEU1 for restoration of the ts phenotype in an SCL1-1 crl3-2 strain. The scl1+ mRNA is about 900 nt long and encodes an open reading frame of 810 bp. The polypeptide deduced from scl1+ possesses a putative secretory signal peptide. The 5'-noncoding region may be under multiple controls, since it contains significant homology to the consensus sequences for the DNA-binding proteins, GCN4, GFI and, possibly, TUF. Gene disruption of scl1+ demonstrates that it is an essential gene.
authors.affiliation: Unité de Biochimie Physiologique, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.
authors.forename: E
authors.initials: E
authors.lastname: Balzi
authors.forename: W N
authors.initials: WN
authors.lastname: Chen
authors.forename: E
authors.initials: E
authors.lastname: Capieaux
authors.forename: J H
authors.initials: JH
authors.lastname: McCusker
authors.forename: J E
authors.initials: JE
authors.lastname: Haber
authors.forename: A
authors.initials: A
authors.lastname: Goffeau
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D005799:Genes, Dominant; D005800:Genes, Fungal; D005804:Genes, Lethal; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids; D012331:RNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D013489:Suppression, Genetic; D014170:Transformation, Genetic
chemical_list: D004271:DNA, Fungal; D012331:RNA, Fungal