Affinity resins for the purification of opioid-binding materials.
Two new affinity resins for the purification of opioid-binding materials were prepared. One was AH-Sepharose coupled with [D-Ala2, D-Leu5]enkephalin and the other was AF-Amino Toyopearl with [D-Ala2, Leu5]enkephalin. Solubilized-opioid receptors from rat brain were treated with these affinity resins and the materials with opioid-binding activities were purified. On sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis, the purified materials showed one major band with a molecular weight of 62000-64000. The results suggested that the prepared resins are useful tools for the purification of opioid receptors.
Fujioka
T
T
Inoue
F
F
Kuriyama
M
M
D000818:Animals; D001921:Brain / Q000378:metabolism; D055598:Chemical Phenomena; D002621:Chemistry; D004743:Enkephalin, Leucine / Q000031:analogs & derivatives / Q000302:isolation & purification; D016308:Enkephalin, Leucine-2-Alanine / Q000031:analogs & derivatives; D066298:In Vitro Techniques; D009294:Narcotics / Q000032:analysis / Q000302:isolation & purification*; D051381:Rats; D012116:Resins, Plant / Q000032:analysis
D009294:Narcotics; D012116:Resins, Plant; D004743:Enkephalin, Leucine; D016308:Enkephalin, Leucine-2-Alanine; C030279:enkephalinamide-Leu, Ala(2)-