Measurement of leukocyte elastase/alpha 1-proteinase-inhibitor complex using a homogeneous and a heterogeneous enzyme-immunoassay.
The heterogeneous 2 h elastase/alpha 1-proteinase-inhibitor enzyme-linked immunosorbent assay was compared with a newly developed homogeneous 20 min immunoactivation test. The within-run and between-days coefficients of variation for both tests were satisfactory. Good agreement was found between the two tests, using specimens from 140 patients. In conclusion, the 20 min immunoactivation test is able to replace the 2 h elastase/alpha 1-proteinase-inhibitor enzyme-linked immunosorbent assay.
Fink
P C
PC
Zentralkrankenhaus Bremen.
Suin de Boutemard
C
C
Haeckel
R
R
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000704:Analysis of Variance; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D019272:Leukocyte Elastase; D008297:Male; D008875:Middle Aged; D010196:Pancreatic Elastase / Q000032:analysis* / Q000378:metabolism; D000515:alpha 1-Antitrypsin / Q000032:analysis* / Q000378:metabolism
D000515:alpha 1-Antitrypsin; C037741:alpha 1-antitrypsin-leukocyte elastase complex; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase