The location of DNA in RecA-DNA helical filaments.
The helical filament that the RecA protein of Escherichia coli forms around DNA is the active apparatus in protein-catalyzed homologous genetic recombination. The actual position of DNA within this complex has been unknown. Image analysis has been performed on electron micrographs of filaments of RecA on double-stranded DNA and on single-stranded DNA to visualize a difference that is consistent with one strand of the double-stranded DNA. This localization of the DNA gives additional information about the unusual structure of DNA in the complex with RecA protein.
Egelman
E H
EH
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511.
Yu
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X
D004269:DNA, Bacterial / Q000032:analysis* / Q000648:ultrastructure; D004277:DNA, Single-Stranded / Q000032:analysis* / Q000648:ultrastructure; D004926:Escherichia coli / Q000235:genetics*; D005583:Fourier Analysis; D007091:Image Processing, Computer-Assisted; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D011938:Rec A Recombinases / Q000032:analysis* / Q000648:ultrastructure; D011995:Recombination, Genetic
D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D046911:Macromolecular Substances; D011938:Rec A Recombinases