title: [Leakage measurements with the dye extraction method and direct methotrexate level determination (enzyme immunoassay) during and after regional cytostatic limb perfusion].
abstract: The dye stuff extinction-method stood the test for checking the separation between the regional and the systemic circulation during the perfusion of extremity tumors by cytostatic drugs. On the other hand the use of an enzyme immunoassay for direct methotrexate-level measuring in serum is recommendable in the postoperative phase. Complications by methotrexate are recognized early by means of an enzyme immunoassay and an individualized application is possible.
authors.affiliation: Chirurgische Klinik und Poliklinik, des Bereichs Medizin der Martin-Luther-Universität Halle-Wittenberg.
authors.forename: J
authors.initials: J
authors.lastname: Krauss
authors.forename: G
authors.initials: G
authors.lastname: Krause
authors.forename: G
authors.initials: G
authors.lastname: Hellthaler
mesh_terms: D010478:Chemotherapy, Cancer, Regional Perfusion; D004395:Dye Dilution Technique; D005121:Extremities; D006801:Humans; D007124:Immunoenzyme Techniques; D008545:Melanoma / Q000628:therapy*; D008727:Methotrexate / Q000032:analysis*; D012878:Skin Neoplasms / Q000628:therapy*
chemical_list: D008727:Methotrexate
vernacular_title: Abstrommessungen mit der Farbstoffextinktionsmethode und der direkten Methotrexatspiegelbestimmung (Enzymimmunoassay) während und nach der regionalen zytostatischen Extremitätenperfusion.