Surface immunoglobulin on lymphocytes of the tortoise Agrionemys horsfieldii.
Surface immunoglobulin bearing (sIg+) cells were identified in the tortoise Agrionemys horsfieldii by indirect immunofluorescence by using class-specific antisera to tortoise IgM and IgY produced in mice. With this technique 13-23% of blood lymphocytes were found to bear surface IgM and 5-8% IgY. Spleen lymphocytes show a higher percentage of sIg+ cells: 25-52% sIgM+ and 5-20% sIgY+ lymphocytes. There is strong indication that tortoise sIgY+ lymphocytes of blood and spleen also express IgM on their surface.
Andreas
E M
EM
Laboratory of Immunobiology, Karl Marx University, Leipzig, G.D.R.
Ambrosius
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H
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D007122:Immunoelectrophoresis; D007075:Immunoglobulin M / Q000032:analysis*; D007136:Immunoglobulins / Q000032:analysis*; D008214:Lymphocytes / Q000276:immunology*; D011947:Receptors, Antigen, B-Cell / Q000032:analysis*; D013154:Spleen / Q000166:cytology; D014426:Turtles / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; C016512:IgY; D007075:Immunoglobulin M; D007136:Immunoglobulins; D011947:Receptors, Antigen, B-Cell