A balance between self-renewal and commitment in the murine erythroleukemia cells with the transferred c-myc gene; an in vitro stochastic model.
When murine erythroleukemia (MEL) cells, having the transferred rat c-myc gene under the control of human metallothionein II gene promoter, are induced to differentiate with dimethyl sulfoxide (DMSO), the level of differentiation is dependent on the c-myc levels which are modulated by the Zn++ ion. The clonal transformant cell line (38-2) can continuously grow in the presence of both DMSO and Zn++ ion. The proportion of differentiated cells in a population of the continuous culture is strongly affected by the concentration of Zn++ ions. These results suggested that a balance between self-renewal and commitment to differentiation of MEL cells is determined by the c-myc level, and that this cell line may be suitable for studying the stochastic process of growth and differentiation of hemopoietic stem cells.
Yamamoto
T
T
Department of Cell Biology, Tohoku University, Sendai, Japan.
Masuko
K
K
Takada
S
S
Kume
T U
TU
Obinata
M
M
D000818:Animals; D002461:Cell Line, Transformed; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D015870:Gene Expression / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D004915:Leukemia, Erythroblastic, Acute / Q000453:epidemiology / Q000235:genetics / Q000473:pathology*; D051379:Mice; D011336:Probability; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc; D013269:Stochastic Processes; D014170:Transformation, Genetic / Q000187:drug effects; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000473:pathology
D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D004121:Dimethyl Sulfoxide