title: Electrostatic interactions in the assembly of Escherichia coli aspartate transcarbamylase.
abstract: Although ionizable groups are known to play important roles in the assembly, catalytic, and regulatory mechanisms of Escherichia coli aspartate transcarbamylase, these groups have not been characterized in detail. We report the application of static accessibility modified Tanford-Kirkwood theory to model electrostatic effects associated with the assembly of pairs of chains, subunits, and the holoenzyme. All of the interchain interfaces except R1-R6 are stabilized by electrostatic interactions by -2 to -4 kcal-m-1 at pH 8. The pH dependence of the electrostatic component of the free energy of stabilization of intrasubunit contacts (C1-C2 and R1-R6) is qualitatively different from that of intersubunit contacts (C1-C4, C1-R1, and C1-R4). This difference may allow the transmission of information across subunit interfaces to be selectively regulated. Groups whose calculated pK or charge changes as a result of protein-protein interactions have been identified and the results correlated with available information about their function. Both the 240s loop of the c chain and the region near the Zn(II) ion of the r chain contain clusters of ionizable groups whose calculated pK values change by relatively large amounts upon assembly. These pK changes in turn extend to regions of the protein remote from the interface. The possibility that networks of ionizable groups are involved in transmitting information between binding sites is suggested.
authors.affiliation: Department of Molecular Biology and Biochemistry, Wesleyan University, Middletown, Connecticut 06457.
authors.forename: M P
authors.initials: MP
authors.lastname: Glackin
authors.forename: M P
authors.initials: MP
authors.lastname: McCarthy
authors.forename: D
authors.initials: D
authors.lastname: Mallikarachchi
authors.forename: J B
authors.initials: JB
authors.lastname: Matthew
authors.forename: N M
authors.initials: NM
authors.lastname: Allewell
mesh_terms: D000596:Amino Acids / Q000032:analysis; D001221:Aspartate Carbamoyltransferase / Q000096:biosynthesis*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004734:Energy Metabolism; D004926:Escherichia coli / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D013438:Sulfhydryl Compounds / Q000032:analysis
chemical_list: D000596:Amino Acids; D013438:Sulfhydryl Compounds; D001221:Aspartate Carbamoyltransferase