Detection of antiplatelet immunoglobulin in thrombocytopenic dogs.
Indirect enzyme-linked immunosorbent assays (ELISA) were used to detect platelet-associated immunoglobulin in sera from dogs with immune-mediated thrombocytopenia (IMT) and/or other autoimmune syndromes. One ELISA, utilizing whole platelets as the antigen substrate, readily detected antibody associated with platelets, either as specific, antiplatelet antibody or as immune complexes. This assay apparently lacked specificity because of the position reactions with sera from dogs with miscellaneous autoimmune disorders and no concurrent thrombocytopenia. Although the second ELISA, utilizing immunoaffinity purified platelet antigens was not influenced as much by immune complexes, absorbance values apparently were slightly increased. However, a small number of dogs with non-thrombocytopenic autoimmune disease tested positive. Immunoadsorption and Western immuno-blotting techniques demonstrated a complex pattern of specificities for antiplatelet antibodies. Clinical significance of these findings is discussed.
McVey
D S
DS
Department of Laboratory Medicine, College of Veterinary Medicine, Kansas State University, Manhattan 66506.
Shuman
W S
WS
D000818:Animals; D000918:Antibody Specificity; D001323:Autoantibodies / Q000032:analysis*; D001324:Autoantigens / Q000276:immunology / Q000302:isolation & purification; D001327:Autoimmune Diseases / Q000276:immunology / Q000662:veterinary*; D001792:Blood Platelets / Q000276:immunology*; D015153:Blotting, Western / Q000662:veterinary; D004283:Dog Diseases / Q000276:immunology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D007163:Immunosorbent Techniques / Q000662:veterinary; D008297:Male; D013921:Thrombocytopenia / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*
D001323:Autoantibodies; D001324:Autoantigens