A cosmid clone map derived from a small region of human chromosome 11.
We have used cosmid "fingerprinting" to construct an overlapping DNA clone "map" of the human DNA in a mouse/human hybrid cell line, E65-9, that contains about 4 x 10(6) bp, including the H-Ras gene, as its human component. We have additionally used 32P-labeled RNA probes to establish linkage of particular sets of clones, and the final map comprises about 300,000 bp and is contained in three nonoverlapping segments. The reasons for failure to close the gaps by direct probing are discussed. We have developed techniques to search for rare cutting restriction enzyme cleavage sites in large numbers of cloned DNAs and have positioned sites for EagI and BssHII on our clone map. The methods we used are capable of considerable scale-up and are currently being applied to the short arm of human chromosome 11.
Harrison-Lavoie
K J
KJ
Department of Biochemistry, Imperial College, London, United Kingdom.
John
R M
RM
Porteous
D J
DJ
Little
P F
PF
D000818:Animals; D002880:Chromosomes, Human, Pair 11; D003001:Cloning, Molecular; D003360:Cosmids; D011905:Genes, ras; D008040:Genetic Linkage; D006801:Humans; D006822:Hybrid Cells; D015151:Immunoblotting; D051379:Mice; D009710:Nucleotide Mapping; D011401:Promoter Regions, Genetic; D015347:RNA Probes
D015347:RNA Probes