Immunochemical detection of Z-DNA in rat pachytene spermatocytes.
Rat testicular nuclei have been probed for the presence of Z-DNA conformation by employing indirect immunofluorescence technique using anti-Z-DNA antibodies. Pachytene nuclei, in which meiotic recombination takes place, showed brighter fluorescence than the premeiotic and postmeiotic spermatogenic nuclei. Moreover, utilizing a novel chromatin immunoblotting technique, Z-DNA conformation was found to be enriched in the poly(ADP-ribosyl)ated chromatin domains of the pachytene nucleus.
Satyanarayana
K
K
Department of Biochemistry, Indian Institute of Science, Bangalore.
Rao
M R
MR
D000818:Animals; D000906:Antibodies; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure; D002843:Chromatin / Q000032:analysis / Q000648:ultrastructure; D004247:DNA / Q000032:analysis / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D008297:Male; D009243:NAD / Q000378:metabolism; D009690:Nucleic Acid Conformation; D011064:Poly Adenosine Diphosphate Ribose / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013090:Spermatocytes / Q000166:cytology*
D000906:Antibodies; D002843:Chromatin; D009243:NAD; D011064:Poly Adenosine Diphosphate Ribose; D004247:DNA