title: New suppressors of signal-sequence mutations, prlG, are linked tightly to the secE gene of Escherichia coli.
abstract: Analysis of more than 100 extragenic suppressors of the lamB14D signal-sequence mutation (changes Val in the hydrophobic core region at position 14 to Asp) has revealed alterations that appear to lie at prlA (secY) and secA (prlD), two loci known to be mutable to suppressor alleles, and a new suppressor termed prlG. One allele of the new suppressor class, prlG1, has been characterized in some detail. This suppressor counteracts, to some degree, the export defect conferred by a variety of signal-sequence mutations in two different genes, lamB and malE. Genetic analysis shows that the dominant suppressor mutations are linked tightly to, and probably allelic with, the gene secE. This result, coupled with data obtained with conditional-lethal alleles of secE, argues strongly that SecE is an important component of the cellular protein export machinery in Escherichia coli.
authors.affiliation: Department of Biology, Princeton University, New Jersey 08544.
authors.forename: J
authors.initials: J
authors.lastname: Stader
authors.forename: L J
authors.initials: LJ
authors.lastname: Gansheroff
authors.forename: T J
authors.initials: TJ
authors.lastname: Silhavy
mesh_terms: D001426:Bacterial Proteins; D002874:Chromosome Mapping; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005796:Genes; D005798:Genes, Bacterial; D005799:Genes, Dominant; D008040:Genetic Linkage; D007700:Kinetics; D009154:Mutation; D010641:Phenotype; D021382:Protein Sorting Signals / Q000235:genetics*; D000069816:SEC Translocation Channels; D013489:Suppression, Genetic
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D021382:Protein Sorting Signals; D000069816:SEC Translocation Channels; C065493:SecE protein, E coli