Immunoelectron microscopic studies of type X collagen in endochondral ossification.
Immunofluorescence and immunoelectron microscopy were used in conjunction with a monoclonal antibody to investigate the localization of type X collagen in the proximal tibial growth plate of 7-d-old chicks. This molecule was detected throughout the hypertrophic zone first appearing when chondrocytes exhibited hypertrophy: it was absent from the proliferative zone. Type X collagen was primarily associated with type II collagen fibrils as demonstrated by immunogold staining. Type X collagen was not concentrated in the focal calcification sites nor was it associated with matrix vesicles. These observations suggest that type X collagen may play a role other than that directly related to the nucleation of calcification.
Poole
A R
AR
Joint Diseases Laboratory, Shriners Hospital for Crippled Children, Montreal, Quebec, Canada.
Pidoux
I
I
D000818:Animals; D001846:Bone Development; D002130:Calcium Phosphates / Q000032:analysis; D002455:Cell Division; D002645:Chickens; D003094:Collagen / Q000032:analysis / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D006132:Growth Plate / Q000166:cytology / Q000648:ultrastructure*; D008854:Microscopy, Electron
D002130:Calcium Phosphates; D003094:Collagen