Burn wound closure with cultured autologous keratinocytes and fibroblasts attached to a collagen-glycosaminoglycan substrate.
Improved survival of patients with extensive burn injuries requires the development of better techniques for timely and permanent closure of the full-thickness wound. We developed procedures for establishing confluent, stratified layers of cultured, autologous keratinocytes on the surface of a modified collagen-glycosaminoglycan membrane that contains autologous fibroblasts. We transferred the composite grafts onto limited areas of excised full-thickness burn wounds of four patients with extensive burn injuries. Our results indicate that this composite graft material has acceptable "take," with formation of a basement membrane within 9 days of graft placement. Use of this technique for major wound coverage may offer a significant advance in the care of extensively burned patients and also may be useful for providing skin for reconstructive surgery.
Hansbrough
J F
JF
Department of Surgery, University of California, San Diego Medical Center.
Boyce
S T
ST
Cooper
M L
ML
Foreman
T J
TJ
D000328:Adult; D002056:Burns / Q000473:pathology / Q000628:therapy*; D002478:Cells, Cultured; D002986:Clinical Trials as Topic; D003094:Collagen / Q000008:administration & dosage*; D005260:Female; D005347:Fibroblasts / Q000637:transplantation*; D006025:Glycosaminoglycans / Q000008:administration & dosage*; D006801:Humans; D015603:Keratinocytes / Q000637:transplantation*; D008297:Male; D008567:Membranes, Artificial; D008875:Middle Aged; D012867:Skin / Q000648:ultrastructure; D014945:Wound Healing
D006025:Glycosaminoglycans; D008567:Membranes, Artificial; D003094:Collagen