title: Transfer of foreign genes by electroporation and their expression in mammalian cells.
abstract: Expression of foreign genes transferred into mammalian cells by electroporation has been studied. The pX1TK gene, pSV2Neo gene and pUCEJ oncogene have been introduced into MLTK- cells and NIH/3T3 cells, respectively. Stable transformation transient expression of TK gene by MLTK- cells as well as stable and malignant transformation of NIH/3T3 cells have been obtained. Transient expression frequency is about 80% and stable transformation frequency is about 10(-4). Integration of foreign genes into the cellular genome was verified with molecular hybridization. Tumor development was observed after inoculation of transformed cells into nude mice.
authors.affiliation: Institute of Cancer, Chinese Academy of Medical Sciences, Beijing.
authors.forename: J T
authors.initials: JT
authors.lastname: Yang
authors.forename: X Q
authors.initials: XQ
authors.lastname: Wang
authors.forename: M
authors.initials: M
authors.lastname: Wu
mesh_terms: D000818:Animals; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D011905:Genes, ras; D051379:Mice; D008819:Mice, Nude; D010957:Plasmids; D014162:Transfection