title: Cloning and characterization of an Escherichia coli gene, pcnB, affecting plasmid copy number.
abstract: A gene, pcnB, affecting the copy number of ColE1-related plasmids has been cloned and mapped to 3.6 min on the Escherichia coli chromosome between panD and fhu. The gene encodes a previously undescribed 48 kD protein. Several independently isolated mutants exhibiting the same phenotype, reduced copy number, have been shown to be pcnB-.
authors.affiliation: Department of Molecular Biology, University of Edinburgh.
authors.forename: J B
authors.initials: JB
authors.lastname: March
authors.forename: M D
authors.initials: MD
authors.lastname: Colloms
authors.forename: D
authors.initials: D
authors.lastname: Hart-Davis
authors.forename: I R
authors.initials: IR
authors.lastname: Oliver
authors.forename: M
authors.initials: M
authors.lastname: Masters
mesh_terms: D000483:Alleles; D000668:Ampicillin Resistance; D001426:Bacterial Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D008970:Molecular Weight; D009154:Mutation; D010641:Phenotype; D010957:Plasmids; D014176:Protein Biosynthesis; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014161:Transduction, Genetic
chemical_list: D001426:Bacterial Proteins