title: Nucleotide sequence of the maltotetraohydrolase gene from Pseudomonas saccharophila.
abstract: The nucleotide sequence of the Pseudomonas saccharophila gene encoding maltotetraohydrolase (G4-forming amylase) has been determined. The coding region for the G4-forming amylase precursor contained 1653 nucleotides. The deduced precursor protein included an N-terminal 21-residue putative signal peptide; the deduced mature form of G4-forming amylase contains 530 amino acid residues with a calculated molecular mass of 57 740 Da. Sequence similarities between the G4-forming amylase and other amylolytic enzymes of species ranging from prokaryotes to eukaryotes are quite limited. However, three regions, which are involved in both the catalytic and substrate-binding sites of various amylolytic enzymes, are highly conserved in the G4-forming amylase of P. saccharophila.
authors.affiliation: Institute of Applied Biochemistry, University of Tsukuba, Japan.
authors.forename: J H
authors.initials: JH
authors.lastname: Zhou
authors.forename: T
authors.initials: T
authors.lastname: Baba
authors.forename: T
authors.initials: T
authors.lastname: Takano
authors.forename: S
authors.initials: S
authors.lastname: Kobayashi
authors.forename: Y
authors.initials: Y
authors.lastname: Arai
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001483:Base Sequence; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D005815:Genetic Code; D006868:Hydrolysis; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis; D011498:Protein Precursors / Q000032:analysis; D021382:Protein Sorting Signals / Q000032:analysis; D011549:Pseudomonas / Q000201:enzymology*; D000520:alpha-Glucosidases / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D000596:Amino Acids; D010446:Peptide Fragments; D011498:Protein Precursors; D021382:Protein Sorting Signals; D000520:alpha-Glucosidases; C020135:glucan 1,4-maltotetraohydrolase