Maturational changes in the LH response of domestic fowl to synthetic chicken LHRH-I and -II.
Treatment of chickens at different stages of sexual development with a single i.v. injection of synthetic chicken LHRH (cLHRH)-I or -II stimulated a rise in the plasma concentration of LH within 1 min. The activity of cLHRH-II was 1.3- to 2.7-fold greater than that of cLHRH-I in sexually immature cockerels and hens as determined by the changes in the plasma concentration of LH during the 5 or 10 min after injection. This could be attributed to both a greater effectiveness of cLHRH-II to stimulate LH release and to a more prolonged action. Thus, LH concentrations in plasma were maximal within 1-2 min of injection of all doses of cLHRH-I but within 2-5 min of injection at the higher doses of cLHRH-II. The responsiveness of the pituitary gland to cLHRH-I and -II was substantially greater in the sexually immature cockerel than in the hen and diminished during sexual development of the hen. Coincident with the onset of egg laying, the characteristics of the LH response to cLHRH-II changed to consist of an initial rise during the first 2 min, followed by a more sustained increase with LH concentrations still rising 10 min after injection. In contrast, after injection with cLHRH-I, plasma concentrations of LH rose to a peak at 2 min and thereafter declined gradually. Treatment of the sexually immature hen with oestradiol, progesterone or a combination of both steroids did not enable the expression of a laying hen-type response to the injection of cLHRH-II.(ABSTRACT TRUNCATED AT 250 WORDS)
Wilson
S C
SC
Department of Biochemistry & Physiology, School of Animal and Microbial Sciences, University of Reading, Whiteknights.
Cunningham
F J
FJ
Chairil
R A
RA
Gladwell
R T
RT
D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens / Q000097:blood / Q000502:physiology*; D004347:Drug Interactions; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives* / Q000494:pharmacology; D007986:Luteinizing Hormone / Q000097:blood*; D008297:Male; D008969:Molecular Sequence Data; D011374:Progesterone / Q000494:pharmacology; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D012741:Sexual Maturation / Q000502:physiology*
D007987:Gonadotropin-Releasing Hormone; C037700:LHRH, Gln(8)-; D011374:Progesterone; D004958:Estradiol; D007986:Luteinizing Hormone; C047717:LHRH, His(5)-Trp(7)-Tyr(8)-; D011761:Pyrrolidonecarboxylic Acid