title: Protease-catalyzed semisynthesis of human neuropeptide Y.
abstract: Human neuropeptide Y was semisynthesized by enzymatic condensation of des-Tyr36-NH2 human neuropeptide Y and H-Tyr-NH2 using Pseudomonas aeruginosa elastase, a metalloenzyme possessing a hydrolytic specificity for the imino side of hydrophobic amino acids. The optimum pH for this enzymatic synthesis was judged to be around 7 in a high concentration of an organic solvent.
authors.forename: K
authors.initials: K
authors.lastname: Sakina
authors.forename: S
authors.initials: S
authors.lastname: Funakoshi
authors.forename: H
authors.initials: H
authors.lastname: Yajima
mesh_terms: D000595:Amino Acid Sequence; D006801:Humans; D008969:Molecular Sequence Data; D009478:Neuropeptide Y / Q000096:biosynthesis*; D010447:Peptide Hydrolases / Q000378:metabolism*
chemical_list: D009478:Neuropeptide Y; D010447:Peptide Hydrolases