A complete deletion mutant of the Escherichia coli dnaKdnaJ operon.
Southern hydridization analyses of genomic DNAs from various dnaJ mutants of Escherichia coli showed that mutant K7052, which has well characterized dnaK706 and dnaJ705 double mutantions, is a deletion mutant. The deletion is about 8.0 kb long and encompasses the whole of the dnaKdnaJ operon.
Ohki
R
R
Department of Molecular Biology, School of Health Sciences, Kyorin University, Hachioji, Japan.
Morita
R
R
Kawamata
T
T
Uchida
H
H
Ohki
M
M
D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics*; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; D004926:Escherichia coli / Q000235:genetics*; D006360:Heat-Shock Proteins / Q000235:genetics*; D009154:Mutation; D009693:Nucleic Acid Hybridization; D009876:Operon; D015183:Restriction Mapping
D004269:DNA, Bacterial; D006360:Heat-Shock Proteins; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; C061224:endodeoxyribonuclease PvuI; D015252:Deoxyribonucleases, Type II Site-Specific