title: Automated enzyme immunoassay for lutropin with the Abbott IMx analyzer.
abstract: An automated enzyme immunoassay for human lutropin for use with the Abbott IMx analyzer is described. The assay provides results in approximately 40 min with a sensitivity of 0.25 int. units of LH per liter for up to 23 serum or plasma samples. Cross-reactivity with follitropin (2000 int. units/L) and thyrotropin (2 int. units/L) was negligible; it was 0.016% with human choriogonadotropin (1 X 10(6) int. units/L). There was no interference by high concentrations of bilirubin (0.5 g/L), hemoglobin (7.50 g/L), or triglycerides (13.5 g/L). Intra-, inter-, and total assay CVs were less than or equal to 3.75%, less than or equal to 7.1%, and less than or equal to 7.94%, respectively. Values obtained with the IMx correlated well (r = 0.98, n = 194) with values obtained with Diagnostic Products' LH Double Antibody RIA, and Serono's LH MAIAclone assay. This assay should be useful for small to medium-size laboratories involved in the clinical diagnosis of reproductive pathology.
authors.affiliation: Department of Physiological Diagnostics, Abbott Diagnostics Division, Abbott Laboratories, Abbott Park, IL 60064.
authors.forename: K A
authors.initials: KA
authors.lastname: Ford
authors.forename: H N
authors.initials: HN
authors.lastname: Baker
authors.forename: J G
authors.initials: JG
authors.lastname: Baar
authors.forename: D
authors.initials: D
authors.lastname: Balay
authors.forename: D
authors.initials: D
authors.lastname: Marlewski
authors.forename: M
authors.initials: M
authors.lastname: Venetucci
authors.forename: E A
authors.initials: EA
authors.lastname: Ogunro
mesh_terms: D000568:Amenorrhea / Q000097:blood; D001322:Autoanalysis / Q000295:instrumentation; D003429:Cross Reactions; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007986:Luteinizing Hormone / Q000097:blood*; D008297:Male; D008593:Menopause; D008597:Menstrual Cycle; D011627:Puberty; D011933:Reagent Kits, Diagnostic; D013223:Statistics as Topic
chemical_list: D011933:Reagent Kits, Diagnostic; D007986:Luteinizing Hormone