Mass measurement of inositol phosphates.
This review summarises the methods available for the mass measurement of inositol phosphates, i.e., use of radioactive inositol lipid precursors, optical techniques, gas chromatography, mass spectrometry, nuclear magnetic resonance, fast atom bombardment and assays specific for Ins(1,4,5)P3. Examples of the use of each method, its sensitivity, advantages and drawbacks are given.
Palmer
S
S
Department of Biochemistry, University of Glasgow, U.K.
Wakelam
M J
MJ
D000818:Animals; D002849:Chromatography, Gas; D006801:Humans; D015544:Inositol 1,4,5-Trisphosphate / Q000032:analysis*; D007295:Inositol Phosphates / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008832:Microchemistry; D008970:Molecular Weight; D011868:Radioisotopes
D007295:Inositol Phosphates; D011868:Radioisotopes; D015544:Inositol 1,4,5-Trisphosphate