A rapid and efficient procedure for transformation of intact Saccharomyces cerevisiae by electroporation.
A rapid and efficient procedure is described for transforming Saccharomyces cerevisiae using electroporation to render intact cells permeable to DNA. The technique uses relatively low voltages and is particularly sensitive to low concentrations of plasmid DNA. At the highest voltage used (400 volts), the frequency of transformation increased with the amount of plasmid DNA between 25 ng and 100 ng. At higher concentrations of DNA (1-1.5 micrograms) electroporation yielded one-third to one-half the number of transformants obtained with a standard lithium acetate pretreatment. Because this method requires neither pretreatment of cells nor addition of polyethylene glycol (PEG), it has several advantages over currently used transformation procedures.
Simon
J R
JR
Department of Biological Chemistry, UCLA School of Medicine 90024.
McEntee
K
K
D004558:Electric Stimulation / Q000379:methods; D004926:Escherichia coli / Q000235:genetics; D007700:Kinetics; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014170:Transformation, Genetic