title: Mutagenicity on chlorination of products formed by ozonation of naphthoresorcinol in water.
abstract: The mutagenicity of products formed by chlorination after ozonation of naphthoresorcinol in aqueous solution was assayed with Salmonella typhimurium strains TA98 and TA100 in the presence and absence of S9 mix from phenobarbital- and 5,6-benzoflavone-induced rat liver. Ozonated and subsequently chlorinated naphthoresorcinol was directly mutagenic, as was ozonated naphthoresorcinol, in both strains tested. The mutagenic activity at chlorination with 8 equivalents of chlorine per mole of naphthoresorcinol after ozonation was markedly higher than that at only ozonation. Of the identified ozonation products of naphthoresorcinol, muconic acid, after chlorination with 2 or 4 equivalents of chlorine per mole of the compound, induced direct mutagenicity against TA98 and TA100. The chlorination of glyoxal with 0.5 and 1 chlorine equivalents per mole of the compound was shown to produce direct mutagenicity toward TA98. The identification of the chlorination products of these compounds is also discussed.
authors.affiliation: Division of Environmental Health, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Sayato
authors.forename: K
authors.initials: K
authors.lastname: Nakamuro
authors.forename: H
authors.initials: H
authors.lastname: Ueno
mesh_terms: D000818:Animals; D001711:Biotransformation; D055598:Chemical Phenomena; D002621:Chemistry; D002713:Chlorine; D006037:Glyoxal / Q000493:pharmacokinetics / Q000633:toxicity; D008862:Microsomes, Liver / Q000378:metabolism; D009152:Mutagenicity Tests; D009153:Mutagens / Q000493:pharmacokinetics; D009284:Naphthols / Q000493:pharmacokinetics / Q000633:toxicity*; D010126:Ozone; D051381:Rats; D012486:Salmonella typhimurium / Q000235:genetics; D013011:Sorbic Acid / Q000031:analogs & derivatives / Q000493:pharmacokinetics / Q000633:toxicity; D014873:Water Pollutants / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
chemical_list: D009153:Mutagens; D009284:Naphthols; D014873:Water Pollutants; D014874:Water Pollutants, Chemical; C005939:muconic acid; D002713:Chlorine; D006037:Glyoxal; C027029:naphthoresorcinol; D010126:Ozone; D013011:Sorbic Acid