title: Direct visualization of an IgM directed against a membrane antigen involved in both cell-matrix interactions and microfilament organization.
abstract: Dental mesenchymal cells were cultured in the presence of a monoclonal antibody. MC16A16, consisting of IgM and directed against a hydrophobic 165 kDa protein. Since the epitope recognized by MC16A16 was found to be at the outer cell surface, a direct visualization of IgM antibodies was used to localize the 165 kDa antigen by transmission electron microscopy. The present results demonstrate that the 165 kDa antigen is a membrane protein.
authors.affiliation: Institut de Biologie Médicale, CNRS LP 6520, Faculté de Médecine 11, Strasbourg, France.
authors.forename: H
authors.initials: H
authors.lastname: Lesot
authors.forename: J M
authors.initials: JM
authors.lastname: Meyer
authors.forename: A
authors.initials: A
authors.lastname: Staubli
authors.forename: D A
authors.initials: DA
authors.lastname: Schmitt
authors.forename: J V
authors.initials: JV
authors.lastname: Ruch
mesh_terms: D008841:Actin Cytoskeleton / Q000648:ultrastructure; D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D007075:Immunoglobulin M; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000032:analysis; D051379:Mice; D008854:Microscopy, Electron; D008970:Molecular Weight; D017830:Octoxynol; D011092:Polyethylene Glycols; D014070:Tooth / Q000166:cytology*
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D007075:Immunoglobulin M; D008565:Membrane Proteins; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40