title: Distribution and function of non-lymphoid cells positive for monoclonal antibody CVI-ChNL-68.2 in healthy chickens and those infected with Marek's disease virus.
abstract: Immuno-enzyme histochemistry was used to study the staining pattern and tissue distribution of monoclonal antibody CVI-ChNL-68.2 that specifically reacts with a subset of non-lymphoid cells in healthy chickens and those infected with Marek's disease virus (MDV). Functional characteristics of CVI-ChNL-68.2-positive cells, e.g. antigen uptake, are determined. In the liver CVI-ChNL-68.2 recognizes reticulum cells, whereas in the bursa of Fabricius it detects single cells in the interfollicular connective tissue. In the spleen CVI-ChNL-68.2 reacts selectively with the reticulum cells of the ellipsoid. In some MDV-infected chickens the splenic reticulum cells show a different staining and distribution pattern. Furthermore, the proliferative lesions associated with Marek's disease contain many CVI-ChNL-68.2-positive cells. The possible role of CVI-ChNL-68.2-positive cells in disseminating Marek's disease virus is discussed.
authors.affiliation: Central Veterinary Institute, Dept. of Virology, AJ Lelystad, The Netherlands.
authors.forename: S H
authors.initials: SH
authors.lastname: Jeurissen
authors.forename: E M
authors.initials: EM
authors.lastname: Janse
authors.forename: G L
authors.initials: GL
authors.lastname: Kok
authors.forename: G F
authors.initials: GF
authors.lastname: De Boer
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002060:Bursa of Fabricius / Q000276:immunology; D002645:Chickens / Q000276:immunology*; D007124:Immunoenzyme Techniques; D008099:Liver / Q000276:immunology; D008297:Male; D008380:Marek Disease / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000276:immunology; D014018:Tissue Distribution
chemical_list: D000911:Antibodies, Monoclonal