title: Monoclonal antibodies to enzymes.
abstract: The production, identification, and utilisation of monoclonal antibodies to enzymes are reviewed. Such antibodies may be produced in vitro by the mouse-hybridoma technique, may occur naturally in vivo as enzyme-binding immunoglobulins and may be produced in the laboratory from the lymphocytes of patients whose sera contains such immunoglobulins. The diagnostic application of monoclonal antibodies to enzymes is considered, with special reference to their use in the measurement of the MB-isoenzyme of creatine kinase, pancreatic isoamylase, prostatic acid phosphatase, and the isoenzymes of alkaline phosphatase.
authors.affiliation: Department of Chemical Pathology and Human Metabolism Royal Free Hospital, London, UK.
authors.forename: S B
authors.initials: SB
authors.lastname: Rosalki
mesh_terms: D000135:Acid Phosphatase / Q000097:blood* / Q000276:immunology; D000469:Alkaline Phosphatase / Q000097:blood* / Q000276:immunology; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D003402:Creatine Kinase / Q000097:blood* / Q000276:immunology; D006026:Glycoside Hydrolases / Q000097:blood*; D006801:Humans; D007517:Isoamylase / Q000097:blood* / Q000276:immunology; D007527:Isoenzymes / Q000097:blood / Q000276:immunology; D008297:Male; D010179:Pancreas / Q000201:enzymology; D011467:Prostate / Q000201:enzymology
chemical_list: D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D003402:Creatine Kinase; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D006026:Glycoside Hydrolases; D007517:Isoamylase