Proliferation of human malignant melanomas is inhibited by antisense oligodeoxynucleotides targeted against basic fibroblast growth factor.
Human malignant melanomas, unlike normal melanocytes, can proliferate in the absence of exogenous basic fibroblast growth factor (bFGF). Exposure of primary melanomas in the vertical growth phase and metastatic melanomas to antisense oligodeoxynucleotides targeted against three different sites of human bFGF mRNA inhibited cell proliferation and colony formation in soft-agar. In contrast, exposure of human bFGF sense or antisense oligonucleotides complementary to human beta-nerve growth factor or insulin-like growth factor I mRNA had no such effects. These experiments indicate that activation of the bFGF gene may play an important role in the progression from melanocytic precursor lesions to malignant melanoma.
Becker
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Department of Tumor Biology, M.D. Anderson Cancer Center, Houston, TX 77030.
Meier
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CB
Herlyn
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M
D000970:Antineoplastic Agents / Q000494:pharmacology*; D001483:Base Sequence; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003114:Colony-Forming Units Assay; D005346:Fibroblast Growth Factors / Q000037:antagonists & inhibitors / Q000235:genetics*; D006131:Growth Inhibitors / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D008545:Melanoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000378:metabolism / Q000494:pharmacology*; D016376:Oligonucleotides, Antisense; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D006131:Growth Inhibitors; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; D005346:Fibroblast Growth Factors