title: Evidence for differences in low density lipoprotein processing by porcine granulosa and luteal cells in vitro: effect of addition of serum for plating of granulosa cells on lipoprotein metabolism.
abstract: The present studies were carried out to compare the low density lipoprotein (LDL) metabolism by freshly isolated immature porcine granulosa cells with that by luteal cells. Furthermore, we have examined the effect of serum used for plating of granulosa cells on lipoprotein degradation and utilization. In incubation studies, addition of LDL as an exogenous substrate had a mild stimulatory effect on progesterone accumulation by granulosa cells, while it exhibited a dose-dependent stimulatory effect on luteal cells. When granulosa and luteal cells were incubated with 125I-labelled LDL, membrane binding of LDL occurred in both cell types, but only luteal cells were capable of internalizing the bound LDL. Granulosa cells in incubation degraded LDL much less in comparison with luteal cells, and the amount varied with the maturity of the cells. When granulosa cells were plated with graded amounts of serum which was withdrawn for 48 h following plating, they exhibited enhanced LDL degradation in a serum concentration-dependent fashion. Addition of serum for plating selectively enhanced utilization of LDL, but not high density lipoprotein (HDL) for progesterone accumulation by the cells in culture. Time-course studies on LDL degradation by granulosa cells following serum withdrawal indicate that the ability of cells to degrade LDL decreased in a time-dependent fashion. Serum withdrawal selectively decreased utilization of LDL but not HDL for progesterone secretion. It is concluded that immature granulosa cells have a limited capability to utilize cholesterol carried by LDL.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Obstetrics and Gynecology, University of Saskatchewan, Saskatoon, Canada.
authors.forename: K
authors.initials: K
authors.lastname: Rajkumar
authors.forename: H
authors.initials: H
authors.lastname: Ly
authors.forename: P W
authors.initials: PW
authors.lastname: Schott
authors.forename: B
authors.initials: B
authors.lastname: Njaa
authors.forename: B D
authors.initials: BD
authors.lastname: Murphy
mesh_terms: D000818:Animals; D001769:Blood; D002454:Cell Differentiation; D003338:Corpus Luteum / Q000378:metabolism*; D003584:Cytological Techniques; D004305:Dose-Response Relationship, Drug; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism*; D008075:Lipoproteins, HDL / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism*; D011374:Progesterone / Q000378:metabolism; D013552:Swine; D013997:Time Factors
chemical_list: D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D011374:Progesterone