Preliminary crystallographic study of glycosylated recombinant human renin.
Single crystals of glycosylated recombinant human renin have been obtained using the hanging-drop vapor diffusion method with polyethylene glycol and sodium chloride as coprecipitants. The crystals belong to the cubic space group P2(1)3 with a = 143.0 A and contain two molecules of renin in the asymmetric unit. A self-rotation function study using 5.5 A data shows the orientation of a non-crystallographic 2-fold axis relating these two monomers.
Lim
L W
LW
Corporate Research and Development, Monsanto, Chesterfield Village, MO 63198.
Stegeman
R A
RA
Leimgruber
N K
NK
Gierse
J K
JK
Abdel-Meguid
S S
SS
D006031:Glycosylation; D006801:Humans; D011994:Recombinant Proteins / Q000378:metabolism / Q000648:ultrastructure; D012083:Renin / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014961:X-Ray Diffraction
D011994:Recombinant Proteins; D012083:Renin