An antiserum to the N-terminal subsequence of the Alzheimer amyloid beta protein does not react with neurofibrillary tangles.
Polyclonal antibodies were raised against a synthetic peptide corresponding to a subsequence for the first 10 residues of the beta amyloid protein A4 (1-10 beta PA4). In an immunoperoxidase study of Alzheimer brain tissue, these antibodies immunostained senile plaque cores, amyloid vessel walls, and amyloid fibrils surrounding senile plaques and angiopathic vessels. Neurofibrillary tangles stained with thioflavin S or immunostained with anti-Tau immune serum were never immunodetected with the anti 1-10 beta PA4. We confirm that the neurofibrillary tangles do not contain epitopes corresponding to the first 10 residues of the beta PA4.
Behrouz
N
N
National Institute of Health and Medical Research (INSERM 156), University of Lille II, France.
Defossez
A
A
Delacourte
A
A
Hublau
P
P
Mazzuca
M
M
D000544:Alzheimer Disease / Q000276:immunology* / Q000473:pathology; D000682:Amyloid / Q000276:immunology*; D016229:Amyloid beta-Peptides; D000818:Animals; D000917:Antibody Formation; D000937:Antigen-Antibody Reactions; D002540:Cerebral Cortex / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D009454:Neurofibrils / Q000276:immunology*; D011817:Rabbits
D000682:Amyloid; D016229:Amyloid beta-Peptides