The stimulation of IL-2 production by anti-rheumatic drugs.
IL-2 release from mouse splenocytes was measured by assaying the IL-2 on an IL-2-dependent cytotoxic T-lymphocyte line in culture (CTLL). Proliferation of the CTLL cells was monitored indirectly with the dye thiazolyl blue. The slow-acting anti-rheumatic drug auranofin at concentrations below 0.1 microM potentiated concanavalin A (Con A)-induced IL-2 release. Similar potentiation of Con A-induced IL-2 release was obtained with D-penicillamine, 1 microM-1 mM, and with the angiotensin-converting enzyme-inhibitor captopril, 10 nM-1 microM. Potentiation of Con A-induced IL-2 release was obtained with concentrations of the drugs likely to be achieved in vivo during therapy. Auranofin but not D-penicillamine and captopril inhibited Con A-induced IL-2 release at high concentrations (greater than 0.3 microM).
Oben
J A
JA
Department of Pharmacology, University College London, U.K.
Wallace
G R
GR
Chain
B M
BM
Foreman
J C
JC
D000818:Animals; D001310:Auranofin / Q000494:pharmacology*; D002216:Captopril / Q000494:pharmacology*; D002478:Cells, Cultured; D007376:Interleukin-2 / Q000096:biosynthesis*; D051379:Mice; D010396:Penicillamine / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology / Q000187:drug effects
D007376:Interleukin-2; D001310:Auranofin; D002216:Captopril; D010396:Penicillamine