title: Voltage-dependent gating properties of the channel formed by E. coli hemolysin in planar lipid membranes.
abstract: Escherichia coli hemolysin forms cation selective, ion-permeable channels of large conductance in planar phospholipid bilayer membranes. The pore formation mechanism is voltage dependent resembling that of some colicins and of diphtheria toxin: pores open when negative voltages are applied and close with positive potentials. The pH dependence of this gating process suggests that it is mediated by a negative fixed charge present in the lumen of the pore. A simple physical model of how the channel opens and closes in response to the applied voltage is given.
authors.affiliation: Dipartimento di Fisica, Universita' di Trento, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Menestrina
authors.forename: M
authors.initials: M
authors.lastname: Ropele
mesh_terms: D004558:Electric Stimulation; D004926:Escherichia coli / Q000472:pathogenicity*; D006460:Hemolysin Proteins / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D015640:Ion Channel Gating / Q000502:physiology*; D008051:Lipid Bilayers; D008954:Models, Biological
chemical_list: D006460:Hemolysin Proteins; D008051:Lipid Bilayers