title: Analysis of the expression of two phosphoglycerate kinase genes in a mouse cultured cell line during activation and inactivation of the c-myc gene.
abstract: The effect of a change in the concentration of c-myc protein on the expression of genes for two phosphoglycerate kinase isozymes was investigated. The steady state levels of messenger ribonucleic acids (mRNAs) for sperm-type and non-sperm-type proteins were determined by blot hybridization using the RNA of the mouse cell line 38-2 containing the inducible rat c-myc gene cultured under various conditions. Without induction the c-myc gene. mRNA for non-sperm-type protein was detected at a level that remained essentially constant during both activation and inactivation of the c-myc gene. mRNA for sperm-type protein was not detected in 38-2 cells cultured under any conditions used. Change in the amount of c-myc protein alone does not appear to bring about a switch of the expression of the two phosphoglycerate kinase genes during spermatogenesis in mouse testis.
authors.forename: S
authors.initials: S
authors.lastname: Koikeda
authors.forename: M
authors.initials: M
authors.lastname: Tamaru
authors.forename: M
authors.initials: M
authors.lastname: Goto
authors.forename: N
authors.initials: N
authors.lastname: Mori
authors.forename: S
authors.initials: S
authors.lastname: Takada
authors.forename: M
authors.initials: M
authors.lastname: Obinata
authors.forename: Y
authors.initials: Y
authors.lastname: Masamune
authors.forename: Y
authors.initials: Y
authors.lastname: Nakanishi
mesh_terms: D002460:Cell Line; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D010735:Phosphoglycerate Kinase / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics; D016271:Proto-Oncogene Proteins c-myc; D011519:Proto-Oncogenes
chemical_list: D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D004247:DNA; D010735:Phosphoglycerate Kinase