Antinuclear antibody in juvenile rheumatoid arthritis sera reacts with 50-40 kDa antigen(s) found in HeLa nuclear extracts.
To identify whether sera containing antinuclear antibody (ANA) from patients with juvenile rheumatoid arthritis (JRA) recognize a common antigen, extracts of HeLa nuclei were prepared by incubating the nuclei with buffers of sequentially increasing salt concentrations (0.1 M, 0.3 M, and 0.5 M NaCl). Western blot analysis using sera from 82 patients with JRA revealed that 63% of ANA positive JRA sera reacted with 2 bands (50-40 kDa) in the 0.3 M extract whereas only 14% of ANA negative JRA sera had antibody to these bands. Antibody to the 50-40 kDa antigen(s) strongly correlated with the presence of ANA by immunofluorescence and was found in 70% of patients with iritis; however, it did not correlate with disease subgroup or disease activity.
Haber
P L
PL
Department of Internal Medicine, Cardinal Glennon Children's Hospital, St. Louis, MO.
Osborn
T G
TG
Moore
T L
TL
D000974:Antibodies, Antinuclear / Q000032:analysis*; D000941:Antigens / Q000276:immunology*; D001171:Arthritis, Juvenile / Q000097:blood / Q000145:classification / Q000276:immunology*; D002467:Cell Nucleus / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D003240:Connective Tissue Diseases / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells / Q000032:analysis*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D008970:Molecular Weight; D014020:Tissue Extracts / Q000276:immunology*
D000974:Antibodies, Antinuclear; D000941:Antigens; D007074:Immunoglobulin G; D014020:Tissue Extracts