[The use of the plasmid pTH10 for isolating the donor strains of Pseudomonas mallei].
The plasmid pTH10 was transfered by conjugation into the Pseudomonas mallei strains. An attempt to construct the donor strains using the widely known technique employing the homology between the plasmid and chromosome due to the transposon Tn1 carried by the plasmid was unsuccessful. Among the clones resistant to bacteriophage PRD1 the variants were selected with the supposed integration of the plasmid into the chromosome. The latter clones required the ability to transfer the auxotrophic chromosomal markers in conjugation after the repeated conjugational transfer of the plasmid pTH10 into them.
Ageeva
N P
NP
Merinova
L K
LK
Peters
M K
MK
D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005819:Genetic Markers; D008242:Lysogeny; D010641:Phenotype; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics*
D004269:DNA, Bacterial; D005819:Genetic Markers
Primenenie plazmidy pTH10 dlia polucheniia donornykh shtammov Pseudomonas mallei.