Effects of ascorbic acid on the antifungal action of amphotericin B.
Ascorbic acid enhanced the lethal but not the permeabilizing effects of amphotericin B on Candida albicans and Cryptococcus neoformans cells. Two other ene-diol acids, D-erythorbate and dihydroxyfumarate, also enhanced the lethal action of amphotericin B on Can. albicans. Maleic acid and gulanolactone, compounds structurally related to ascorbic acid but not containing the ene-diol group, had no such effect. It is assumed that ascorbic acid and the two other ene-diol acids acting as pro-oxidants augmented the oxidation-dependent killing of fungal cells induced by amphotericin B.
Brajtburg
J
J
Division of Infectious Diseases, Washington University School of Medicine, St. Louis, MO 63110.
Elberg
S
S
Kobayashi
G S
GS
Medoff
G
G
D000666:Amphotericin B / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects / Q000378:metabolism; D003455:Cryptococcus neoformans / Q000187:drug effects / Q000378:metabolism; D003470:Culture Media; D005658:Fungi / Q000187:drug effects* / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D011188:Potassium / Q000378:metabolism
D003470:Culture Media; D000666:Amphotericin B; D001205:Ascorbic Acid; D011188:Potassium