Preparative purification of Plasmodium falciparum circumsporozoite protein synthetic polypeptides by displacement chromatography.
Displacement chromatography was used for the preparative purification of a synthetic polypeptide that is a promising malaria vaccine. It was prepared by solid-phase synthesis and contains two important epitopes of circumsporozoite (CS) protein of Plasmodium falciparum sporozoite. With apparatus typically employed in analytical high-performance liquid chromatography (HPLC) and on a 250 x 4.6 mm I.D. reversed-phase column, up to 50 mg of crude polypeptide were purified in a single run and with a yield higher than 95%. The results demonstrate that displacement chromatography is suitable for the isolation of several milligrams of a pure polypeptide from a complex mixture that is difficult to separate even by analytical HPLC. In such a preparative application, displacement appears to be superior to elution chromatography as used traditionally.
Viscomi
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GC
Laboratory of Peptide Synthesis, SCLAVO, Rome, Italy.
Ziggiotti
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Verdini
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AS
D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D008969:Molecular Sequence Data; D010455:Peptides / Q000302:isolation & purification*; D010963:Plasmodium falciparum / Q000276:immunology*; D015800:Protozoan Proteins
D000953:Antigens, Protozoan; D010455:Peptides; D015800:Protozoan Proteins; C041595:circumsporozoite protein, Protozoan