[Acute experimental glomerulonephritis induced by the glomerular deposition of circulating polymeric IgA-concanavalin A complexes].
The perfusion of polymeric IgA (pIgA)--or secretory IgA (sIgA)--Concanavalin A (ConA) non-immune complexes (apparent Mol.Wt greater than 10(3) Kd) into the aorta of rats led to a dose-dependent and a mannose-dependent deposition of both IgA and lectin into the glomeruli. Rats injected with amounts of those complexes as low as 500 micrograms developed, one hour later, a focal and segmental proliferative glomerulonephritis characterized by: a) the deposition in the mesangial area of most glomeruli of injected complexes and of rat C3; b) small areas of fibrinoïd necrosis in 10 to 15% of glomeruli, confined to the periphery of a single lobule of the tuft; c) a segmental infiltration of those glomeruli by polymorphonuclear leucocytes, mononuclear phagocytes and platelets; d) a hyperactive aspect of mesangial cells; e) the presence of red blood cells in tubular lumens. By contrast, no glomerular lesions were obvious in rats similarly injected either with monomeric IgA (mIgA)-ConA, pIgA-peanut agglutinin (PNA) or sIgA-PNA complexes or with heat-aggregated pIgA or mIgA. The data indicate that preformed polymeric IgA-ConA complexes can specifically bind to glomerular structures in vivo and locally trigger, as antigen-antibody complexes, an acute inflammatory reaction resulting in glomerular lesions similar to those observed in Henoch-Schönlein purpura nephritis.
Davin
J C
JC
Université de Liège, Belgique.
Dechenne
C
C
Mahieu
P R
PR
D000208:Acute Disease; D000818:Animals; D003208:Concanavalin A / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D005455:Fluorescent Antibody Technique; D005922:Glomerulonephritis, IGA / Q000276:immunology* / Q000473:pathology; D007070:Immunoglobulin A / Q000276:immunology*; D007678:Kidney Glomerulus / Q000276:immunology* / Q000473:pathology; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains
D007070:Immunoglobulin A; C034639:polymeric IgA; D003208:Concanavalin A
Glomérulonéphrite aiguë expérimentale induite par le dépôt glomérulaire de complexes circulants IgA polymérique-concanavaline A.