title: Direct evidence for a protein recognized by a monoclonal antibody against oxidatively modified LDL in atherosclerotic lesions from a Watanabe heritable hyperlipidemic rabbit.
abstract: Low density lipoproteins (LDL) that have been oxidatively modified have been implicated in the pathogenesis of atherosclerosis. Monoclonal antibodies were generated against oxidatively modified human low density lipoproteins (OxLDL); these antibodies reacted with OxLDL, but did not react with native LDL, either in an enzyme-linked immunosorbent assay (ELISA) or a Western blot analysis. The anti-OxLDL antibodies did react with other modified forms of LDL (eg, acetylated LDL, malondialdehyde-modified LDL, and cell-modified LDL) that were recognized by the scavenger receptor on macrophages. Single- and double-label immunofluorescence of atheromatous lesions from a Watanabe heritable hyperlipidemic (WHHL) rabbit demonstrated some colocalization of proteins detected by anti-LDL and anti-OxLDL antibodies. However, clearly there were also areas stained by the anti-OxLDL antibodies that did not stain with anti-LDL. Staining of the lesion by the anti-OxLDL antibody was abolished by adsorption of the antibody with OxLDL, but not by adsorption with LDL or bovine serum albumin. Arterial tissue from a control New Zealand White rabbit did not show staining with anti-LDL or anti-OxLDL antibodies. These observations suggest that OxLDL (or possibly other proteins recognized by the anti-OxLDL antibody) is present in atheromatous lesions of WHHL rabbits, and are consistent with oxidatively modified lipoproteins having a role in atherogenesis.
authors.affiliation: Department of Pathology, University of Washington, Seattle 98195.
authors.forename: H C
authors.initials: HC
authors.lastname: Boyd
authors.forename: A M
authors.initials: AM
authors.lastname: Gown
authors.forename: G
authors.initials: G
authors.lastname: Wolfbauer
authors.forename: A
authors.initials: A
authors.lastname: Chait
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001011:Aorta / Q000032:analysis; D001161:Arteriosclerosis / Q000209:etiology / Q000378:metabolism*; D015153:Blotting, Western; D005455:Fluorescent Antibody Technique; D006949:Hyperlipidemias / Q000150:complications / Q000378:metabolism*; D007163:Immunosorbent Techniques; D008077:Lipoproteins, LDL / Q000032:analysis* / Q000276:immunology; D010084:Oxidation-Reduction; D011817:Rabbits
chemical_list: D000911:Antibodies, Monoclonal; D008077:Lipoproteins, LDL