[Monoclonal antibody-based immunoenzyme test system for determining the total immunoglobulin E level of children and adolescents].
Enzyme immunoassay for determination of total immunoglobulin E in children and adolescents based on combination of two monoclonal antibodies with high affinity and specificity to different epitopes of IgE molecule has been developed. Monoclonal antibodies IgE/11-4 are used for sensitization of a solid phase (96-well plate), mcAB IgE/11-1 conjugated with horseradish peroxidase are used as a developing reagent. The following analytical characteristics of a test-system are established: range of assay--1-100 IU/ml sensitivity--1 IU/ml; time of analysis--1.5 hour, sample volume in duplicates--30 microliters. Immunoassay is simple, reliable and can be used in the field of pediatrics and allergology.
Serdiuk
O A
OA
Tsytsikov
E N
EN
Vasilov
R G
RG
D000293:Adolescent; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D000918:Antibody Specificity; D000925:Anticoagulants / Q000494:pharmacology; D002648:Child; D005069:Evaluation Studies as Topic; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation; D007073:Immunoglobulin E / Q000032:analysis*
D000911:Antibodies, Monoclonal; D000925:Anticoagulants; D007073:Immunoglobulin E
Immunofermentnaia test-sistema na osnove monoklonal'nykh antitel dlia opredeleniia obshchego urovnia immunoglobulina E u deteĭ i podrostkov.