High levels of c-myc protein in human breast tumours determined by a sensitive ELISA technique.
We have previously examined the expression of (H-ras) oncogene related transcripts (Spandidos and Agnantis: Anticancer Res 4: 269-272, 1984) and also the activation of the H-ras oncogene (Spandidos: Anticancer Res 7: 991-996, 1987) in human malignant breast cancers. Our results revealed overexpression of the H-ras transcripts in malignant compared to normal tissues, while in the same tumour specimens certain point mutations and other genetic changes have been found in the H-ras gene. In this paper we have investigated c-myc oncoprotein expression in the same tumour samples using an ELISA technique (Moore et al: Oncogene Res 2: 65-80, 1989). Elevated c-myc oncoprotein expression was found in all of the tumour specimens compared with normal breast tissues from a patient with no evidence of the disease. A correlation was found between T stage and c-myc expression in both the normal tissue from the resection margin and in the breast tumour specimens. However, no correlation was found between survival and elevated c-myc expression.
Spandidos
D A
DA
Beatson Institute for Cancer Research, Bearsden, Glasgow, Scotland, UK.
Field
J K
JK
Agnantis
N J
NJ
Evan
G I
GI
Moore
J P
JP
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D001940:Breast / Q000032:analysis; D001943:Breast Neoplasms / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005500:Follow-Up Studies; D011905:Genes, ras; D006801:Humans; D008875:Middle Aged; D011379:Prognosis; D011518:Proto-Oncogene Proteins / Q000032:analysis*; D016271:Proto-Oncogene Proteins c-myc; D011519:Proto-Oncogenes; D012016:Reference Values
D014408:Biomarkers, Tumor; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc