title: Altered mRNA cap recognition activity of initiation factor 4E in the yeast cell cycle division mutant cdc33.
abstract: The mutation in the S. cerevisiae cell cycle division mutant cdc33 consists of a single G to A transition in the open reading frame encoding translation initiation factor 4E (eIF-4E). This leads to the substitution of glycine 113 by aspartic acid close to tryptophane 115 in the protein. This mutation reduces cap binding activity of eIF-4E as measured by binding of eIF-4E to m7GDP agarose columns and slows down overall protein synthesis at the non-permissive temperature. Comparison of the cdc33 mutation with other mutations affecting eIF-4E function supports the view that tryptophane residues and their flanking regions are involved in cap binding activity of eIF-4E.
authors.affiliation: Institut für Biochemie und Molekularbiologie Universität Bern, Switzerland.
authors.forename: M
authors.initials: M
authors.lastname: Altmann
authors.forename: H
authors.initials: H
authors.lastname: Trachsel
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015153:Blotting, Western; D002455:Cell Division; D003001:Cloning, Molecular; D039561:Eukaryotic Initiation Factor-4E; D008969:Molecular Sequence Data; D009154:Mutation; D010448:Peptide Initiation Factors / Q000235:genetics / Q000378:metabolism*; D014176:Protein Biosynthesis; D012315:RNA Caps / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics*; D013329:Structure-Activity Relationship; D014364:Tryptophan
chemical_list: D039561:Eukaryotic Initiation Factor-4E; D010448:Peptide Initiation Factors; D012315:RNA Caps; D012333:RNA, Messenger; D014364:Tryptophan