Both CheA and CheW are required for reconstitution of chemotactic signaling in Escherichia coli.
If cells of Escherichia coli deleted for genes that specify transducers and all known cytoplasmic chemotaxis proteins are reconstituted with CheA, CheW, and CheY, they spin their flagella alternately clockwise and counterclockwise. If the aspartate receptor also is present, clockwise rotation is suppressed upon addition of aspartate. If either CheA or CheW is absent, the fraction of time that the flagella spin clockwise is reduced and responses to aspartate do not occur.
Conley
M P
MP
Department of Cellular and Developmental Biology, Harvard University, Cambridge, Massachusetts 02138.
Wolfe
A J
AJ
Blair
D F
DF
Berg
H C
HC
D001426:Bacterial Proteins; D002630:Chemotactic Factors / Q000235:genetics*; D002633:Chemotaxis; D002872:Chromosome Deletion; D004926:Escherichia coli / Q000235:genetics* / Q000502:physiology; D029968:Escherichia coli Proteins; D005796:Genes; D005798:Genes, Bacterial; D000071677:Histidine Kinase; D008565:Membrane Proteins / Q000235:genetics* / Q000502:physiology; D000072236:Methyl-Accepting Chemotaxis Proteins; D009876:Operon; D015183:Restriction Mapping
D001426:Bacterial Proteins; D002630:Chemotactic Factors; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D000072236:Methyl-Accepting Chemotaxis Proteins; C000607606:cheY protein, E coli; D000071677:Histidine Kinase; C000607608:cheA protein, E coli