Fluorogold as a retrograde tracer used in combination with immunohistochemistry.
The fluorescent dye Fluorogold has been recently introduced as a retrograde neuronal tracer that can be used in combination with immunohistochemistry. It has, however, lately been reported that immunohistochemical processes induced rapid photobleaching of Fluorogold. In the present study we prove that it is the water in the sections, and not the immunohistochemical processing itself, that is responsible for the photobleaching. This problem is easily corrected by using a 29:1 or 19:1 glycerine-PBS buffer mounting medium.
Ju
G
G
Department of Neurobiology, Fourth Military Medical university, Xi'an People's Republic of China.
Han
Z S
ZS
Fan
L Z
LZ
D000818:Animals; D001921:Brain / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D007150:Immunohistochemistry / Q000379:methods*; D051381:Rats; D011919:Rats, Inbred Strains; D013266:Stilbamidines
C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D005456:Fluorescent Dyes; D013266:Stilbamidines