Quantification of recombinant interleukin-2 in human serum by a specific immunobioassay.
A sensitive and specific assay for recombinant interleukin-2 (rIL-2) in human serum is described. The assay is based on a sequential sandwich immunobioassay that uses a microtiter plate coated with anti-rIL-2 monoclonal antibody (specific for recombinant human IL-2) to capture rIL-2 from serum, and an IL-2 dependent T-cell line that proliferates in a dose-dependent fashion. The lower limit of quantitation of the assay is 2 units/mL (1 unit = approximately 50 pg) using 0.1 mL of serum and the calibration curves ranged from 2 to 50 units/mL. Data are reported on the sensitivity, precision, reproducibility, and specificity of the assay; the stability of rIL-2 in serum; and the recovery of rIL-2 from serum. We also report on the use of the procedure to assay clinical samples from patients with AIDS undergoing treatment with rIL-2.
Nadeau
R W
RW
Oldfield
N F
NF
Garland
W A
WA
Liberato
D J
DJ
D000163:Acquired Immunodeficiency Syndrome / Q000097:blood; D000911:Antibodies, Monoclonal; D006801:Humans; D007158:Immunologic Techniques; D007376:Interleukin-2 / Q000032:analysis*; D011994:Recombinant Proteins / Q000097:blood
D000911:Antibodies, Monoclonal; D007376:Interleukin-2; D011994:Recombinant Proteins