Chemical composition and tissue distribution of the human CDw44 glycoprotein.
The CDw44 glycoprotein was purified from 2.3 x 10(11) CD3+ CD4+ CD8- T-chronic lymphocytic leukaemia (CLL) cells using F10-44-2 monoclonal antibody affinity chromatography, DEAE-Sepharose anion-exchange chromatography, passage down carboxymethyl (CM)-Sepharose cation-exchange columns, wheat germ lectin affinity chromatography and gel-permeation chromatography. On elution in non-ionic detergents from the DEAE column, two distinct peaks of antigen activity were obtained. The CDw44 glycoprotein in each peak was a glycoprotein of 85,000 MW, but the amino acid composition of the peaks was noticeably different. Carbohydrate compositions showed that each peak contained approximately 30% (w/w) carbohydrate, the composition suggesting both O-linked and complex N-linked glycans. Modulation studies with the F10-44-2 antibody on normal peripheral blood mononuclear cells (PBMC) demonstrated that the CDw44 glycoprotein of T cells consisted of one fraction that was readily modulated, and the other which was resistant to modulation. Detailed tissue distribution studies for CDw44 were performed using the F10-44-2 antibody on frozen sections of human tissues. CDw44 has a restricted tissue distribution, but is found on many highly diverse cell types (e.g. T lymphocytes, smooth muscle cells, some secretory glands, skin epithelial cells).
Flanagan
B F
BF
Blond McIndoe Centre, Queen Victoria Hospital, Sussex, U.K.
Dalchau
R
R
Allen
A K
AK
Daar
A S
AS
Fabre
J W
JW
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000302:isolation & purification; D000945:Antigens, Differentiation, T-Lymphocyte / Q000302:isolation & purification; D002236:Carbohydrate Conformation; D004848:Epithelium / Q000276:immunology; D006023:Glycoproteins / Q000302:isolation & purification*; D006801:Humans; D015461:Leukemia, Prolymphocytic, T-Cell / Q000145:classification; D008562:Membrane Glycoproteins / Q000302:isolation & purification*; D009844:Oligosaccharides / Q000302:isolation & purification; D010641:Phenotype; D011487:Protein Conformation; D016201:Receptors, Lymphocyte Homing; D013601:T-Lymphocytes / Q000032:analysis* / Q000145:classification; D014018:Tissue Distribution
D000596:Amino Acids; D000945:Antigens, Differentiation, T-Lymphocyte; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D009844:Oligosaccharides; D016201:Receptors, Lymphocyte Homing