title: Trophoblast-derived immunoregulatory factor: demonstration of the biological function and the physicochemical characteristics of the factor derived from choriocarcinoma cell lines.
abstract: An immunosuppressive factor released by choriocarcinoma cell lines was analyzed in the present study. It inhibited the proliferative responses of human T cells stimulated by lectins or alloantigens. It also blocked the generation of alloreactive cytotoxic T cells. The suppressive activity of the factor was detected in the responses of the T cells costimulated with 1 nM 12-O-tetradecanoyl phorbol 13-acetate and 1 microM A23187, suggesting the possibility that the factor acted on the intracellular signal transduction in T cells rather than interfering with early events such as T cell receptor signal transduction through cell membranes. Moreover, the factor acted directly on T cell proliferation pathways without activation of suppressor cells but did not act on T cell activation pathways. Taken together, all these findings expanded our previous reports on a factor released by normal trophoblasts, indicating the possible identity of the two factors. The physicochemical properties of the choriocarcinoma-derived factor were examined, and the biological significance of the factor during pregnancy was discussed in this paper.
authors.affiliation: Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Matsuzaki
authors.forename: T
authors.initials: T
authors.lastname: Okada
authors.forename: T
authors.initials: T
authors.lastname: Kameda
authors.forename: T
authors.initials: T
authors.lastname: Negoro
authors.forename: F
authors.initials: F
authors.lastname: Saji
authors.forename: O
authors.initials: O
authors.lastname: Tanizawa
mesh_terms: D000001:Calcimycin / Q000494:pharmacology; D002822:Choriocarcinoma / Q000276:immunology*; D005260:Female; D006801:Humans; D008213:Lymphocyte Activation; D013491:Suppressor Factors, Immunologic / Q000032:analysis* / Q000502:physiology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014327:Trophoblasts / Q000276:immunology*; D014407:Tumor Cells, Cultured; D014594:Uterine Neoplasms / Q000276:immunology*
chemical_list: D013491:Suppressor Factors, Immunologic; D000001:Calcimycin; D013755:Tetradecanoylphorbol Acetate