title: The effects of various growth factors on endothelial cell survival in vitro.
abstract: The effects of various growth factors on endothelial cell survival in vitro were studied. Using rat heart endothelial cells, the cell survival curves were obtained; the cells were cultured until confluent, the medium was changed to serum-free medium with or without growth factors, and the cells were counted after 3, 6, 9, and 12 days. Transforming growth factor-beta, which is known as a potent growth inhibitor for vascular endothelial cells, shortened the rat heart endothelial cell's survival period, while epidermal growth factor or transforming growth factor-alpha prolonged survival. Insulin did not affect the rat heart endothelial cell's survival. Our data indicate that growth factors play a role not only in cell proliferation but also in cell survival in vitro. In addition, elevated levels of growth inhibitors such as transforming growth factor-beta may cause tissue damage in vivo by affecting cell survival.
authors.affiliation: Department of Dermatology, Faculty of Medicine, University of Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Etoh
authors.forename: K
authors.initials: K
authors.lastname: Takehara
authors.forename: A
authors.initials: A
authors.lastname: Igarashi
authors.forename: Y
authors.initials: Y
authors.lastname: Ishibashi
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004727:Endothelium / Q000166:cytology*; D004815:Epidermal Growth Factor / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology*; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology; D009206:Myocardium / Q000166:cytology; D051381:Rats; D011994:Recombinant Proteins; D013045:Species Specificity; D013997:Time Factors; D015291:Transforming Growth Factors / Q000494:pharmacology
chemical_list: D006133:Growth Substances; D007328:Insulin; D011994:Recombinant Proteins; D004815:Epidermal Growth Factor; D015291:Transforming Growth Factors