title: Mutants of bacteriophage T7 that escape F restriction.
abstract: Mutants of bacteriophage T7 that escape F restriction have been isolated. Two mutations in gene 10, which codes for the capsid protein, and one mutation in gene 1.2 are required for these phages to grow on F-containing strains. The products of these two genes are the two targets of the exclusion system; the presence of either wild-type product results in an abortive infection. Phages that grow normally in male hosts still lead to membrane dysfunction and nucleotide efflux from the infected cell. This type of membrane damage and the abortive infection are therefore separable phenomena.
authors.affiliation: Department of Microbiology, University of Texas, Austin 78712.
authors.forename: I J
authors.initials: IJ
authors.lastname: Molineux
authors.forename: C K
authors.initials: CK
authors.lastname: Schmitt
authors.forename: J P
authors.initials: JP
authors.lastname: Condreay
mesh_terms: D004279:DNA, Viral / Q000235:genetics; D012321:DNA-Directed RNA Polymerases; D004926:Escherichia coli; D005144:F Factor; D005814:Genes, Viral; D008566:Membranes; D009154:Mutation; D013604:T-Phages
chemical_list: D004279:DNA, Viral; D012321:DNA-Directed RNA Polymerases