title: New crystal form of recombinant murine interferon-beta.
abstract: Although we have reported (Matsuda, S., Kawano, G., Itoh, S., Mitsui, Y., and Iitaka, Y. (1986) J. Biol. Chem. 261, 16207-16209) that recombinant murine interferon-beta produced in Escherichia coli was crystallized in an orthorhombic space group C222(1) using polyethyleneglycol 8000 as precipitant, the crystals had an insufficient resolution and a marked tendency for orientational disorder around the c axis. We now report that another form of murine interferon-beta crystals with little disorder was obtained in the presence of dioxane using ammonium sulfate as precipitant. The new crystals belong to a hexagonal space group P6(1) or P6(5) with a = b = 71.4 A and c = 79.6 A having only one murine interferon-beta molecule in an asymmetric unit. The crystals are reasonably stable to x-rays and significantly diffract up to 2.2 A resolution when a synchrotron beam is used.
authors.affiliation: Basic Research Laboratories, Toray Industries, Inc., Kamakura, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Matsuda
authors.forename: T
authors.initials: T
authors.lastname: Senda
authors.forename: S
authors.initials: S
authors.lastname: Itoh
authors.forename: G
authors.initials: G
authors.lastname: Kawano
authors.forename: H
authors.initials: H
authors.lastname: Mizuno
authors.forename: Y
authors.initials: Y
authors.lastname: Mitsui
mesh_terms: D000818:Animals; D003460:Crystallization; D004926:Escherichia coli / Q000235:genetics; D007370:Interferon Type I / Q000302:isolation & purification; D051379:Mice; D011994:Recombinant Proteins; D014961:X-Ray Diffraction
chemical_list: D007370:Interferon Type I; D011994:Recombinant Proteins