Adipose tissue lipogenesis in growing steers adapted to different levels of feed intake.
The association between feed intake and lipogenic activity in adipose tissue was investigated in growing cattle. Twenty-five 300-kg steers were allotted by BW to one of five levels of intake of a single high-energy corn-corn silage-based diet. Steers were adapted to diets over 4 wk and intakes were adjusted weekly to achieve steady but varying rates of growth. Daily intakes (% of BW) averaged .92, 1.15, 1.64, 2.28 and 2.69 and resulted in growth rates over the final 3 wk of -.28, .07, .71, 1.67 and 1.69 kg/d, respectively. Lipogenic activities in biopsied tissue and circulating concentrations of glucose and insulin were lowest at maintenance feeding and below but increased linearly (P less than .01 for lipogenesis; P less than .1 for glucose and insulin) as intake increased above maintenance. Mean minimal and maximal rates (mumoles.-min(-1).10(6) cells(-1)) or concentrations were fatty acids synthesis ([14C]acetate---fatty acid)), .065 and .723; fatty acid synthetase (NADPH oxidized), .266 and 2.97; lipoprotein lipase (fatty acid released), .048 and .359; glucose (mg/dl), 60.4 and 70.7 and insulin (ng/ml), .70 and 1.66. In a preliminary study with the same 25 steers fed ad libitum, nearly 25% of the variability in adipose tissue lipogenesis was accounted for by variation in feed intake. Results indicate that activities of lipogenic enzymes and lipogenic capacity in growing steers coordinately adapt to the level of feed consumed and that nutrient availability and(or) insulin concentrations may participate in this adaptation.
Mills
S E
SE
Department of Animal Science, Purdue University, West Lafayette, IN 47907.
Lemenager
R P
RP
Horstman
L A
LA
D000273:Adipose Tissue / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D002417:Cattle / Q000378:metabolism*; D004435:Eating / Q000502:physiology*; D064429:Fatty Acid Synthases / Q000378:metabolism; D005227:Fatty Acids / Q000096:biosynthesis*; D005508:Food Deprivation / Q000502:physiology; D007328:Insulin / Q000097:blood; D016018:Least-Squares Analysis; D008071:Lipoprotein Lipase / Q000378:metabolism; D008297:Male
D001786:Blood Glucose; D005227:Fatty Acids; D007328:Insulin; D064429:Fatty Acid Synthases; D008071:Lipoprotein Lipase