[Cloning and expression of the hemagglutinin gene of influenza virus subtype H1 in Escherichia coli].
The full-length copy of the hemagglutinin gene of influenza virus was inserted into M13 phage DNA. The DNA sequence coding for the hydrophobic prepeptide was removed from the gene by oligonucleotide-directed mutagenesis. The possibilities of expression of the full-length and mutant genes in E. coli were investigated. The beta-galactosidase-hemagglutinin fusion proteins were isolated. The fusion proteins exhibited specific binding to antiviral antibodies. This binding could be competitively inhibited by excess of viral hemagglutinin, demonstrating that these fusion proteins contained antigenic determinants of hemagglutinin.
Petrenko
V A
VA
Kipriianov
S M
SM
Semenova
L N
LN
Akimenko
Z A
ZA
Rukavishnikov
M Iu
MIu
D001435:Bacteriophages / Q000235:genetics; D003001:Cloning, Molecular; D004247:DNA / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D005814:Genes, Viral; D006389:Hemagglutinins, Viral / Q000235:genetics*; D009980:Influenza A virus / Q000235:genetics*; D010957:Plasmids; D011863:Radioimmunoassay; D015183:Restriction Mapping; D014760:Viral Fusion Proteins / Q000235:genetics / Q000302:isolation & purification
D006389:Hemagglutinins, Viral; D014760:Viral Fusion Proteins; D004247:DNA
Klonirovanie i ékspressiia v Escherichia coli gena gemaggliutinina virusa podtipa H1.