title: Insulin stimulates the translocation of protein kinase C in rat adipocytes.
abstract: Insulin-induced changes in protein kinase C were examined in cytosol and membrane fractions of rat adipocytes enzymatically after Mono Q column chromatography and by immunoblotting. During a 5-20 min period of insulin treatment, cytosolic protein kinase C decrease by approximately 50%, whereas membrane protein kinase C increased nearly 2-fold. These findings suggest that insulin stimulates the translocation of protein kinase C in rat adipocytes.
authors.affiliation: James A. Haley Veterans' Hospital, Research Service Tampa, FL 33612.
authors.forename: T
authors.initials: T
authors.lastname: Ishizuka
authors.forename: D R
authors.initials: DR
authors.lastname: Cooper
authors.forename: R V
authors.initials: RV
authors.lastname: Farese
mesh_terms: D000273:Adipose Tissue / Q000201:enzymology*; D000818:Animals; D015153:Blotting, Western; D002451:Cell Compartmentation / Q000187:drug effects; D002462:Cell Membrane / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology*; D008297:Male; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013997:Time Factors
chemical_list: D007328:Insulin; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate