Quantification of soluble HLA class I gene products by an enzyme linked immunosorbent assay.
A simplified enzyme linked immunosorbent assay utilizing an HLA class I framework-specific monoclonal antibody and a polyclonal enzyme linked beta-2 microglobulin specific antiserum has been established for the quantitative measurement of soluble HLA class I molecules. A total of 219 unrelated healthy individuals and 137 members of 28 families typed for HLA were analyzed for their non-membrane bound, i.e. soluble HLA-A,B,C antigens (sHLA-A,B,C). As reported by others, we observed associations of higher or lower sHLA-A,B,C values to particular HLA antigens: High plasma values were observed in probands positive for HLA-A23, A24, A29, Aw33, Bw65, and Cw8 and low values in HLA-B27 and B37 positive individuals. However, as shown by family studies, levels of sHLA-A,B,C were apparently not controlled by the MHC haplotypes alone, since no significant difference between HLA identical siblings and two haplotype different individuals could be detected. Thus, additional non-MHC linked gene(s) may be involved in the release of class I gene products.
Doxiadis
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I
Institute of Immunogenetics, University Hospital of Essen, Federal Republic of Germany.
Westhoff
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U
Grosse-Wilde
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H
D000911:Antibodies, Monoclonal; D004797:Enzyme-Linked Immunosorbent Assay; D005805:Genes, MHC Class I / Q000276:immunology; D015234:HLA-A Antigens / Q000032:analysis; D015235:HLA-B Antigens / Q000032:analysis; D015236:HLA-C Antigens / Q000032:analysis; D006801:Humans; D001613:beta 2-Microglobulin / Q000276:immunology
D000911:Antibodies, Monoclonal; D015234:HLA-A Antigens; D015235:HLA-B Antigens; D015236:HLA-C Antigens; D001613:beta 2-Microglobulin