title: A comparative study of some properties of cytidine deaminase from Escherichia coli and chicken liver.
abstract: The molecular and kinetic properties of cytidine deaminase from E. coli and chicken liver show several interesting differences and similarities: 1. Both enzymes possess an oligomeric structure, and linear kinetics. 2. The chicken liver enzyme is strictly dependent on the presence of reducing agents and presents a microheterogeneity in the pure preparation. 3. Both enzymes display identical specificity and share a rapid-equilibrium random Uni-Bi mechanism of catalysis. 4. The chicken liver enzyme is inhibited competitively by dTTP, CMP and dCMP.
authors.affiliation: Dipartimento di Biologia Cellulare, Università di Camerino, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Vita
authors.forename: T
authors.initials: T
authors.lastname: Cacciamani
authors.forename: P
authors.initials: P
authors.lastname: Natalini
authors.forename: S
authors.initials: S
authors.lastname: Ruggieri
authors.forename: N
authors.initials: N
authors.lastname: Raffaelli
authors.forename: G
authors.initials: G
authors.lastname: Magni
mesh_terms: D000818:Animals; D002645:Chickens / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003564:Cytidine Deaminase / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D007525:Isoelectric Focusing; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D009700:Nucleoside Deaminases / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D009700:Nucleoside Deaminases; D003564:Cytidine Deaminase