A new family of 2-hydroxyacid dehydrogenases.
The NADH-dependent hydroxypyruvate reductase from cucumber and the pdxB gene product of E. coli display significant homology to E. coli D-3-phosphoglycerate dehydrogenase. In contrast, these proteins do not display much similarity with other oxidoreductases or with other 2-hydroxyacid dehydrogenases in particular. On the basis of their relatedness and the structure of their substrates, these three enzymes constitute a new family of 2-hydroxyacid dehydrogenases distinct from malate and lactate dehydrogenase.
Grant
G A
GA
Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri 63110.
D000249:Adenosine Monophosphate / Q000378:metabolism; D000429:Alcohol Oxidoreductases; D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001665:Binding Sites; D002237:Carbohydrate Dehydrogenases / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins; D050537:Hydroxypyruvate Reductase; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D008969:Molecular Sequence Data; D009243:NAD / Q000494:pharmacology; D050543:Phosphoglycerate Dehydrogenase; D010944:Plants / Q000201:enzymology*; D011736:Pyridoxine / Q000096:biosynthesis; D012689:Sequence Homology, Nucleic Acid
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D009243:NAD; D000249:Adenosine Monophosphate; D000429:Alcohol Oxidoreductases; D002237:Carbohydrate Dehydrogenases; C061651:pdxB protein, E coli; D007770:L-Lactate Dehydrogenase; C020669:Glycerate dehydrogenase; D008291:Malate Dehydrogenase; D050537:Hydroxypyruvate Reductase; D050543:Phosphoglycerate Dehydrogenase; D011736:Pyridoxine