title: Time-dependent inactivation of glucose-6-phosphate dehydrogenase from yeast by aluminum.
abstract: Aluminum inhibited yeast glucose-6-phosphate dehydrogenase (EC 1.1.1.49) by a pseudo-first-order reaction. The inhibition was proportional to the incubation time and the concentration of aluminum. Double reciprocal plots gave a straight line with a kinact of 8.3 min-1 and indicated the presence of a binding step prior to inhibition. The kinetic study showed that 1 mol of aluminum was bound per mol of enzyme subunit.
authors.affiliation: Department of Biochemistry, University of Tennessee, Knoxville 37996-0840.
authors.forename: S W
authors.initials: SW
authors.lastname: Cho
authors.forename: J G
authors.initials: JG
authors.lastname: Joshi
mesh_terms: D000535:Aluminum / Q000633:toxicity*; D005954:Glucosephosphate Dehydrogenase / Q000037:antagonists & inhibitors*; D007700:Kinetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013997:Time Factors
chemical_list: D000535:Aluminum; D005954:Glucosephosphate Dehydrogenase