The behaviour of pyruvate dehydrogenase in circulating lymphocytes from diabetic children.
The basal and total pyruvate dehydrogenase activities were assayed in circulating lymphocytes from children with juvenile diabetes at diagnosis and after five days of insulin therapy and from control subjects. In untreated diabetic children, basal and total pyruvate dehydrogenase activities were deeply decreased and both showed very similar values; whereas, in control subjects basal activity was about 30% lower than total activity. In diabetic patients treated with insulin (in vivo situation), both basal and total activity levels were equal or even higher than those of the control subjects. The incubation of lymphocytes from diabetic patients with insulin (5 microU/ml) (in vitro situation) stimulates, but less than in vivo, the basal and total pyruvate dehydrogenase activities.
Rinaudo
M T
MT
Dipartimento di Medicina ed Oncologia Sperimentale-Sezione Biochimica, Università di Torino, Italy.
Curto
M
M
Bruno
R
R
Marino
C
C
Piccinini
M
M
Cerutti
F
F
Mostert
M
M
Sacchetti
C
C
D001786:Blood Glucose / Q000032:analysis; D002096:C-Peptide / Q000097:blood; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000201:enzymology* / Q000652:urine; D005260:Female; D006029:Glycosuria; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology; D007657:Ketone Bodies / Q000652:urine; D008214:Lymphocytes / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D011768:Pyruvate Dehydrogenase Complex / Q000097:blood*
D001786:Blood Glucose; D002096:C-Peptide; D007328:Insulin; D007657:Ketone Bodies; D011768:Pyruvate Dehydrogenase Complex