Cytotoxin activity on Vero cells among Escherichia coli strains associated with diarrhea in cats.
The role of Escherichia coli as a causative agent of diarrhea in cats was investigated. Isolates of E coli from healthy and diarrheic cats were serotyped and investigated for their biochemical characters, production of cytotoxin activity on Vero cells, heat-labile enterotoxin, heat-stable enterotoxin, and hemagglutination of erythrocytes from other animal species. None of 48 investigated strains produced heat-labile enterotoxin or heat-stable enterotoxin, nor did they agglutinate erythrocytes. Most strains were hemolytic and belonged to O-serotypes 2 and 6. Cytotoxin activity on Vero cells was significantly more common and produced in greater amounts among E coli strains isolated from diarrheic cats, and was neutralized by anti-Shiga-like toxin I serum.
Abaas
S
S
Department of Bacteriology, National Bacteriological Laboratory, Stockholm, Sweden.
Franklin
A
A
Kühn
I
I
Orskov
F
F
Orskov
I
I
D000818:Animals; D001427:Bacterial Toxins / Q000032:analysis / Q000096:biosynthesis*; D002371:Cat Diseases / Q000209:etiology / Q000382:microbiology*; D002415:Cats; D003603:Cytotoxins / Q000032:analysis / Q000096:biosynthesis*; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D004768:Enterotoxins / Q000096:biosynthesis; D004926:Escherichia coli / Q000378:metabolism / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D006384:Hemagglutination; D009500:Neutralization Tests / Q000662:veterinary; D022622:Shiga Toxin 1; D014709:Vero Cells; D014774:Virulence
D001427:Bacterial Toxins; D003603:Cytotoxins; D004768:Enterotoxins; D022622:Shiga Toxin 1