Involvement of transferrin in the uptake of 67Ga in inflammatory and normal tissues.
The results from gel chromatography and electrophoresis showed that 67Ga is exclusively bound with transferrin both in vitro and in vivo, but high concentrations of sodium citrate strongly inhibited the binding of 67Ga to transferrin. The influence of sodium citrate on the uptake of 67Ga into inflammatory and normal soft tissues was also investigated. Sodium citrate decreased the uptake of 67Ga into the liver and spleen, but had no influence on the uptake of 67Ga into inflammatory tissue. These results suggest that the uptake of 67Ga into normal soft tissues occurs in a transferrin-bound form but into inflammatory tissue in an unbound form.
Ohkubo
Y
Y
Department of Radiopharmacy, Tohoku College of Pharmacy, Sendai, Japan.
Shibuya
A
A
Kohno
H
H
Kubodera
A
A
D000818:Animals; D002850:Chromatography, Gel; D002951:Citrates / Q000378:metabolism; D019343:Citric Acid; D004586:Electrophoresis; D005549:Foreign-Body Reaction / Q000000981:diagnostic imaging*; D005710:Gallium Radioisotopes; D008297:Male; D011877:Radionuclide Imaging; D051381:Rats; D011919:Rats, Inbred Strains; D014168:Transferrin / Q000378:metabolism*
D002951:Citrates; D005710:Gallium Radioisotopes; D014168:Transferrin; D019343:Citric Acid