[Ala6]gastrin-releasing peptide-10: an analogue with dissociated biological activities.
COOH-terminal decapeptide of gastrin-releasing peptide (GRP-10) is a bombesin-like peptide, which has bioactivities to stimulate gastrin, insulin, and glucagon secretion. We have synthesized an analogue of GRP-10 that inhibits GRP-10's stimulation of insulin secretion both in vivo and in vitro and glucagon secretion in vivo, while potentiating the stimulation of gastrin secretion. The amino acid sequence of this peptide is H-Gly-Asn-Trp-Ala-Ala-Gly-His-Leu-Met-NH2 ([Ala6]GRP-10). Because the stimulation of insulin and gastrin secretion by GRP-10 has been ascribed to a direct effect on B- and G-cells, these findings suggest that there are two subtypes of receptors for bombesin-like peptides in mammalian tissues.
Mukai
H
H
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
Kawai
K
K
Suzuki
S
S
Ohmori
H
H
Yamashita
K
K
Munekata
E
E
D000818:Animals; D001839:Bombesin / Q000031:analogs & derivatives* / Q000494:pharmacology; D004285:Dogs; D019886:Gastrin-Releasing Peptide / Q000031:analogs & derivatives*; D005755:Gastrins / Q000378:metabolism; D005934:Glucagon / Q000378:metabolism; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism*; D010446:Peptide Fragments / Q000494:pharmacology*; D010477:Perfusion
D005755:Gastrins; D007328:Insulin; D010446:Peptide Fragments; C060679:gastrin-releasing peptide 10, Ala(6)-; D019886:Gastrin-Releasing Peptide; C040794:neuromedin C; D005934:Glucagon; D001839:Bombesin