Production of leukocyte migration inhibition factor (LIF) and migration stimulation factor (MStF) by CD4+ and CD8+ human lymphocytes subsets and T-cell clones.
The production of the lymphokines leukocyte migration inhibition factor (LIF) and migration stimulation factor (MStF) at the level of CD4+ and CD8+ human lymphocyte subsets was investigated. In a first series of experiments, anti-CD4 and anti-CD8 monoclonal antibodies capable of inhibiting the activation by concanavalin-A (Con-A) of the respective T-cell subset were used. It was observed that when CD8+ cell activation was blocked, LIF was always produced after Con-A activation. When CD4+ cell activation was blocked, MStF was produced in five out of nine experiments (no activity in the other four). The addition of N-acetyl-D-glucosamine to block LIF in supernatants of anti-CD8 treated cells was unable to show evidence of masked MStF activity. In a second series of experiments, T-cell clones were established from continuous growing T-lymphocyte cell lines developed from cultures of Con-A activated normal human leukocyte cultures. The phenotype of 22 clones was determined and their ability to produce LIF or MStF investigated. Four clones produced MStF after Con-A activation and all of them were CD3+, CD4-, CD8+. Three clones produced LIF after Con-A activation and all of them were CD3+, CD4+, CD8-. We conclude that LIF is produced by CD4+ cells and MStF by CD8+ cells.
Méndez-Samperio
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P
Jiménez-Zamudio
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L
Favila-Castillo
L
L
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000494:pharmacology*; D002630:Chemotactic Factors / Q000096:biosynthesis*; D002999:Clone Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D003208:Concanavalin A / Q000494:pharmacology; D006801:Humans; D007961:Leukocyte Migration-Inhibitory Factors / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000096:biosynthesis*; D008264:Macrophages; D013601:T-Lymphocytes / Q000145:classification / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D002630:Chemotactic Factors; D007961:Leukocyte Migration-Inhibitory Factors; D008222:Lymphokines; C053609:macrophage migration enhancement factor; D003208:Concanavalin A