Alumina-phosphate complexes for immobilization of biomolecules.
Organic compounds containing the -PO3H2 function are strongly and specifically adsorbed by aluminum oxide in water within a large range of pH. The reversible character of the interaction allows the adsorbed organic phosphates to be displaced by inorganic phosphate buffers resulting in their purification by an affinity-like chromatographic procedure. The interaction between alumina and selected multifunctional compounds containing a phosphonate group yields a chemically activated alumina-phosphate complex onto which enzymes or other molecules can be immobilized. A number of proteases immobilized on alumina through such phosphate interactions proved to be active in the presence of organic solvents. As a consequence, enzyme-catalyzed peptide synthesis in a water-limited environment and optical resolution of amino acids in water-organic solvent emulsions can be accomplished.
Coletti-Previero
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INSERM (U 58), Montpellier, France.
Previero
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D000327:Adsorption; D000535:Aluminum; D000537:Aluminum Oxide; D000596:Amino Acids / Q000302:isolation & purification; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002846:Chromatography, Affinity; D004800:Enzymes, Immobilized; D010452:Peptide Biosynthesis; D010756:Phosphoric Acids; D013237:Stereoisomerism
D000596:Amino Acids; D004800:Enzymes, Immobilized; D010756:Phosphoric Acids; D000535:Aluminum; D000537:Aluminum Oxide