Stimulatory effects of small intestine mucosal factors on the proliferation and antibody formation of primarily cultured rat splenic lymphocytes.
When male adult rats at the age of 8-10 weeks received 70% partial hepatectomy, a factor stimulating the proliferation and differentiation of rat splenic lymphocytes appeared in the portal blood serum taken 48 h later. This study was undertaken to investigate if this factor was present in the small-intestinal tissues which are functionally related to portal veins. Bovine small-intestine mucosal homogenate was ultracentrifuged to separate the supernatant, which was heated gently to form a precipitate. After the precipitate was removed, the supernatant was dialysed and cooled with ice. The fraction retained by the dialysing membrane was added to the medium consisting of RPMI 1640 and new-born calf serum. When rat splenic lymphocytes were cultured in medium containing the fraction, they proceeded to proliferate and their glucose metabolism increased. In the culture of lymphocytes treated with the precipitates obtained from the supernatant by addition of ammonium sulfate, the same effects on cells were observed. In addition, the numbers of antibody-forming cells increased significantly. These results indicate that a lymphocyte-activating factor was present in the fraction obtained from the extract of small-intestinal mucosa by salting-out using ammonium sulfate.
Takahashi
N
N
Division of Agricultural Chemistry, School of Agriculture, Meiji University, Kanagawa, Japan.
Obata
R
R
D000645:Ammonium Sulfate; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D006498:Hepatectomy; D015850:Interleukin-6; D007421:Intestine, Small / Q000032:analysis / Q000166:cytology; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D008297:Male; D009092:Mucous Membrane / Q000032:analysis / Q000166:cytology; D011506:Proteins / Q000032:analysis / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000166:cytology* / Q000187:drug effects / Q000276:immunology
D015850:Interleukin-6; D011506:Proteins; D000645:Ammonium Sulfate