Effect of insulin induced hypoglycaemia on in vitro uptake of 3-O-methylglucose by rat jejunum.
Acute hypoglycaemia (less than 2.0 mmol/l) selectively increased in vitro 3-O-methylglucose uptake by rat jejunum. In contrast, uptake of 3H-phenylalanine or 59Fe (III) was unchanged in the hypoglycaemic animals. The hypoglycaemia was accompanied by decreased tissue adenosine nucleotide energy charge. The increased uptake was phlorrhidzin-inhibitable and therefore reflected increased Na+ dependent glucose carrier activity. Cycloheximide did not block the increased 3-O-methylglucose uptake, implying that the mechanism is not the result of increased de novo carrier protein synthesis.
Banerjee
A K
AK
Division of Clinical Cell Biology, MRC Clinical Research Centre, Harrow, Middlesex.
Raja
K
K
Peters
T J
TJ
D019325:3-O-Methylglucose; D000818:Animals; D001692:Biological Transport; D007003:Hypoglycemia / Q000139:chemically induced / Q000378:metabolism*; D066298:In Vitro Techniques; D007328:Insulin; D007408:Intestinal Absorption; D007583:Jejunum / Q000378:metabolism*; D008297:Male; D008757:Methylglucosides / Q000493:pharmacokinetics*; D008759:Methylglycosides / Q000493:pharmacokinetics*; D051381:Rats; D011919:Rats, Inbred Strains
D007328:Insulin; D008757:Methylglucosides; D008759:Methylglycosides; D019325:3-O-Methylglucose