Growth curve patterns of Escherichia coli, Serratia marcescens, and Proteus vulgaris submitted to different tigemonam concentrations.
Five strains of Escherichia coli, Serratia marcescens, and Proteus vulgaris were exposed to a new monobactam, tigemonam, in comparison with aztreonam. The study, evaluated by kinetic turbidimetry, has shown that tigemonam exerts a prelytic increase in optical density (OD) similar to that of other beta-lactam antibiotics. The maximal value of the prelytic increase in OD was similar for the two study antibiotics at concentrations of 1, 2, 4, and 8 times the minimum inhibitory concentration, corresponding with filament formation. The OD values varied according to the bacterial species. Bactericidal activity was observed for the three species evaluated. At 6 hours, the killing rate was not dose-related. In conclusion, tigemonam and aztreonam induced first filament formation followed after a few hours by bactericidal activity.
Yourassowsky
E
E
Department of Microbiology, Brugmann University Hospital, Brussels, Belgium.
van der Linden
M P
MP
Lismont
M J
MJ
Crokaert
F
F
D001398:Aztreonam / Q000494:pharmacology; D015169:Colony Count, Microbial; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D006801:Humans; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D008997:Monobactams / Q000494:pharmacology*; D011514:Proteus vulgaris / Q000187:drug effects* / Q000254:growth & development; D012706:Serratia marcescens / Q000187:drug effects* / Q000254:growth & development
D008997:Monobactams; C052020:tigemonam; D001398:Aztreonam