C-myc-S mu rearrangement in a murine plasmacytoma without visible chromosomal translocations.
We report here that one murine plasmacytoma (DCPC 21) arising in a Balb/c mouse implanted i.p. with a Millipore diffusion chamber lacks any known chromosomal translocations. In situ molecular hybridization analysis of the metaphase spreads of DCPC 21, however, revealed that the c-myc gene has moved to the IgH locus on the chromosome 12. Further, an Ig switch region-mediated recombination was detected. The DNA sequencing analysis demonstrated that the c-myc gene has juxtaposed directly to S mu 5'-flanking sequence in a 'head-to-tail' orientation. The results strongly support our notion that the c-myc rearrangement is an essential step in the genesis of murine plasmacytomas, whether or not they have visible chromosomal translocations.
Ohno
S
S
Department of Molecular Immunology, Kanazawa University, Japan.
Migita
S
S
Murakami
S
S
D000818:Animals; D001483:Base Sequence; D002871:Chromosome Banding; D002874:Chromosome Mapping; D005260:Female; D015321:Gene Rearrangement; D005803:Genes, Immunoglobulin; D007621:Karyotyping; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010954:Plasmacytoma / Q000201:enzymology / Q000235:genetics*; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc; D011519:Proto-Oncogenes; D015183:Restriction Mapping; D014178:Translocation, Genetic
D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D011505:Protein-Tyrosine Kinases