title: [Chemical characterization of Escherichia coli K 1 capsular polysaccharide].
abstract: The exact chemical characterization of the prepared Escherichia coli K 1 capsule polysaccharide is necessary and a prerequisite for using this antigen as a screening antigen in the production of monoclonal anti-K 1-antibodies. The K 1-antigen, prepared by phenol-water-extraction, was analyzed by protein, RNA, and neuraminic acid determination. An addition, the antigen was subjected to elementary analysis, infrared- and 13C-NMR-spectroscopy, and gelchromatography. After testing the serological specificity, the K 1-antigen was identified as a high molecular polyneuraminic acid.
authors.affiliation: Institut für Medizinische Mikrobiologie des Bereiches Medizin, Humboldt-Universität zu Berlin.
authors.forename: M
authors.initials: M
authors.lastname: Mehl
authors.forename: R
authors.initials: R
authors.lastname: Starke
authors.forename: B
authors.initials: B
authors.lastname: Kissig
authors.forename: R
authors.initials: R
authors.lastname: Grunow
authors.forename: S
authors.initials: S
authors.lastname: Jahn
authors.forename: G
authors.initials: G
authors.lastname: Schmidt
authors.forename: R
authors.initials: R
authors.lastname: von Baehr
mesh_terms: D000942:Antigens, Bacterial / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D004926:Escherichia coli / Q000032:analysis* / Q000276:immunology; D011135:Polysaccharides, Bacterial / Q000032:analysis* / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial; D000954:Antigens, Surface; C030659:K antigens; D011135:Polysaccharides, Bacterial
vernacular_title: Chemische Charakterisierung des Escherichia coli K 1 Kapselpolysaccharides.