Evidence that the anti-coagulant and lethal properties of a basic phospholipase A2 from snake venom are unrelated.
The basic phospholipase A2 (PLA2) from venom of the African elapid Naja nigricollis was previously shown to have anti-coagulant and lethal properties, both of which were abolished by treatment with p-bromophenacyl bromide (pBP). In the present paper we first report that pBP-treated PLA2 is capable of inhibiting the anti-coagulant activity but not the lethal activity of native PLA2, thus suggesting that both properties might be independent. We then confirm this evidence using PLA2-specific monoclonal immunoglobulins. One of these, called HSF, neutralized the lethal activity but not the anti-coagulant activity, whereas another antibody, called HSP2, inhibited the anti-coagulant activity but not the lethal activity of the PLA2. The data presented in this paper are taken as evidence that the anti-coagulant activity is not implicated in the lethal effects of basic PLA2 from Naja nigricollis.
Chwetzoff
S
S
Service de biochimie, CEN de Saclay, Gif-sur-Yvette, France.
Couderc
J
J
Frachon
P
P
Menez
A
A
D000098:Acetophenones / Q000494:pharmacology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001777:Blood Coagulation / Q000187:drug effects*; D001780:Blood Coagulation Tests; D004546:Elapid Venoms / Q000037:antagonists & inhibitors / Q000276:immunology / Q000633:toxicity*; D009500:Neutralization Tests; D010740:Phospholipases / Q000037:antagonists & inhibitors*; D010741:Phospholipases A / Q000037:antagonists & inhibitors* / Q000276:immunology / Q000633:toxicity; D054467:Phospholipases A2
D000098:Acetophenones; D000911:Antibodies, Monoclonal; D004546:Elapid Venoms; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; C006821:4-bromophenacyl bromide