Modification of cell growth-dependent gene transcript levels by sodium butyrate in serum stimulated fibroblasts.
We have analyzed the effect of sodium butyrate, under conditions which block the transition from G0 to S phase, on the steady-state levels of several cell growth-dependent mRNAs in ts13 cells, a G1-specific temperature sensitive mutant of the BHK cell line. The RNAs studied were: beta-actin, vimentin, ATP/ADP translocase, histone H3 and p1B6, a cell growth-dependent sequence selected from a ts13-derived cDNA library. We found that induction of p1B6 RNA by serum in the presence of butyrate is greater than in its absence. The levels of histone and ATP/ADP translocase transcripts are lower when induced in the presence of butyrate, while beta-actin and vimentin RNA levels are little modified by the drug. Mitoguazone and incubation at the non-permissive temperature which, also, arrest cells at the pre-replicative stage, and hydroxyurea and cytosine arabinoside, which block them at the early S phase, have different effects on the levels of these transcripts.
Aller
P
P
Department of Pathology and Fels Research Institute, Temple University, School of Medicine, Philadelphia, PA 19140.
Hirschhorn
R R
RR
Rius
C
C
D000818:Animals; D001769:Blood; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D002453:Cell Cycle / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003470:Culture Media; D003561:Cytarabine / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects*; D005796:Genes / Q000187:drug effects*; D006918:Hydroxyurea / Q000494:pharmacology; D008935:Mitoguazone / Q000494:pharmacology; D009154:Mutation; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D013696:Temperature
D002087:Butyrates; D003470:Culture Media; D012333:RNA, Messenger; D003561:Cytarabine; D020148:Butyric Acid; D008935:Mitoguazone; D006918:Hydroxyurea