title: Differentiation of the vaccine F-strain from other strains of Mycoplasma gallisepticum by restriction endonuclease analysis.
abstract: The electrophoretic patterns of the nucleic acids (DNA) of Mycoplasma gallisepticum strains digested with the restriction enzymes Bam HI, Eco RI and Hind III were useful for differentiating the vaccine F-strain from other strains of M. gallisepticum. The procedure was more sensitive than the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) technique. The vaccine F-strain, represented by cultures designated F-K810 and F-F2F10 was clearly differentiated from other strains of M. gallisepticum. This procedure may be useful in field studies to determine if the vaccine strain will replace wild-type M. gallisepticum in commercial layers.
authors.affiliation: Department of Epidemiology and Preventive Medicine, School of Veterinary Medicine, University of California, Davis 95616.
authors.forename: M I
authors.initials: MI
authors.lastname: Khan
authors.forename: R
authors.initials: R
authors.lastname: Yamamoto
mesh_terms: D001428:Bacterial Vaccines; D004269:DNA, Bacterial / Q000032:analysis*; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D009174:Mycoplasma / Q000235:genetics* / Q000276:immunology; D015183:Restriction Mapping
chemical_list: D001428:Bacterial Vaccines; D004269:DNA, Bacterial; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII