Gas chromatographic determination of gallopamil and norgallopamil in human plasma.
A highly sensitive gas chromatographic assay is described for the simultaneous determination of gallopamil, a calcium channel blocking agent, and its major metabolite, norgallopamil. A multi-step extraction procedure is employed followed by on-column capillary gas chromatographic analysis using nitrogen-selective detection. Acetylation of norgallopamil is performed to enable accurate quantification of the metabolite. Linearity was achieved over the range 1-50 ng/ml for both analytes. Assay specificity, precision and accuracy were investigated.
Ruggirello
D A
DA
Department of Biopharmaceutics, Schering Research, Pembroke Pines, FL 33023.
Noonan
P K
PK
Gonzalez
M A
MA
Ho
P
P
Wagner
J G
JG
D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D005711:Gallopamil / Q000031:analogs & derivatives* / Q000097:blood*; D006801:Humans
C052471:norgallopamil; D005711:Gallopamil