Long-term culture of canine bone marrow cells.
Conditions that allow the growth of canine marrow cells in long-term marrow culture are described. The quality of the culture conditions was evaluated based on the weekly number of granulocyte-macrophage colony-forming units (CFU-GM) in the nonadherent cell fraction. Using this parameter, the highest number of CFU-GM was obtained when marrow cells were incubated at 37 degrees C in RPMI-1640 or McCoy's 5A medium supplemented with 20% prescreened horse serum without addition of hydrocortisone. CFU-GM colonies could be regularly grown out of the nonadherent cell fraction for 20-31 weeks, after recharging the cultures with 1.5 X 10(7) mononuclear autologous marrow cells 1 week after establishing the stromal cell layer.
Schuening
F G
FG
Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
Storb
R
R
Meyer
J
J
Goehle
S
S
D000818:Animals; D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003470:Culture Media; D004285:Dogs; D005260:Female; D006649:Histocompatibility Antigens / Q000276:immunology; D006854:Hydrocortisone / Q000494:pharmacology; D008297:Male; D008854:Microscopy, Electron; D013696:Temperature; D013997:Time Factors
D003470:Culture Media; D006649:Histocompatibility Antigens; D006854:Hydrocortisone