Genomic DNA with transformation-related activity and melanoma antigen expression.
We have analyzed the mouse melanoma antigen with monoclonal antibodies established by syngeneic immunization. To further understand the structure of this antigen at a molecular level, we have cloned the genomic DNA controlling the expression of melanoma antigen by cosmid library transfection and a monoclonal antibody. In the process of analyzing this DNA fragment we found that it contained a gene related with transformation, which was proved by tumor formation in nude mice inoculated with NIH/3T3 transfectants of this DNA fragment. We discuss the structure of the gene product based on the deduced amino acid sequence of cDNA, which maps the genome bearing transformation-related activity.
Tagawa
M
M
Department of Immunology, School of Medicine, Chiba University, Japan.
Sakamoto
T
T
Tamura
Y
Y
Imai
K
K
Ito
T
T
Matsubara
H
H
Kanno
M
M
Shigemoto
K
K
Koseki
H
H
Taniguchi
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000235:genetics* / Q000276:immunology; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003360:Cosmids; D006801:Humans; D008545:Melanoma / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D014176:Protein Biosynthesis; D012878:Skin Neoplasms / Q000235:genetics*; D014162:Transfection; D014170:Transformation, Genetic
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm