Preparation of monoclonal antibodies against methamphetamine.
15 monoclonal antibodies against methamphetamine (MA) were obtained from hybridoma clones by the fusion of mouse 653 myeloma cells with the spleen cells of the immunized mice. The monoclonal antibodies exhibited different cross-reactivities against MA-related compounds and one antibody (MA-15 Ab) was highly specific against MA. A competitive inhibition enzyme-linked immunosorbent assay was established and the detection limit for MA was approximately 20 ng/ml.
Usagawa
T
T
Research Laboratory, Ube Industries Ltd., Japan.
Nishimura
M
M
Uda
T
T
Nakahara
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis*; D000918:Antibody Specificity; D001202:Ascitic Fluid / Q000032:analysis; D001667:Binding, Competitive; D002459:Cell Fusion; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006825:Hybridomas / Q000378:metabolism; D007132:Immunoglobulin Isotypes / Q000032:analysis; D008694:Methamphetamine / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D007132:Immunoglobulin Isotypes; D008694:Methamphetamine