title: Purification and properties of 5,6-dihydropyrimidine amidohydrolase from calf liver.
abstract: 5,6-Dihydropyrimidine amidohydrolase was isolated from an acetone powder of calf liver and purified to homogeneity. Purification made use of heat treatment, ammonium sulfate fractionation and chromatography on Chelating Sepharose and DEAE-Sepharose with 44% recovery of total activity. The native enzyme has a molecular mass of 217 kDa consisting of four subunits with a molecular mass of 54 kDa each. The amidohydrolase is a metalloenzyme containing one zinc atom/subunit. The enzyme can slowly be inactivated by chelating agents. The kinetic parameters for substrates, 5,6-dihydrouracil, 5,6-dihydrothymine and glutarimide were determined. From log Vmax/KM data, a pKa of 7.6 could be calculated suggesting the formation of a zinc-bound hydroxyl ion which carries out the nucleophilic attack on the C-4 of dihydrouracil.
authors.affiliation: Physiologisch-Chemisches Institut der Universität Würzburg, Federal Republic of Germany.
authors.forename: J
authors.initials: J
authors.lastname: Kautz
authors.forename: K D
authors.initials: KD
authors.lastname: Schnackerz
mesh_terms: D000581:Amidohydrolases / Q000302:isolation & purification* / Q000378:metabolism; D000596:Amino Acids / Q000032:analysis; D000645:Ammonium Sulfate; D000818:Animals; D002417:Cattle; D002852:Chromatography, Ion Exchange; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D046911:Macromolecular Substances; D008970:Molecular Weight; D013379:Substrate Specificity
chemical_list: D000596:Amino Acids; D046911:Macromolecular Substances; D000581:Amidohydrolases; C020047:dihydropyrimidinase; D000645:Ammonium Sulfate