Determination of maduramicin by liquid chromatography with atomic absorption spectrometric detection.
A liquid chromatograph was interfaced to an atomic absorption spectrometer for the detection and quantitation of maduramicin in feed matrixes at the 1-8 ppm level. Ionophores in general form strong 1:1 products with various metal cations, yielding complexes that are insoluble in water but very soluble in organic solvents. Maduramicin, a carboxylic, polyalcohol, polyether antibiotic, is labeled with the sodium cation and analyzed by atomic absorption spectroscopy (AAS). The lower limit of detection is approximately 100-200 ng maduramicin sodium salt. Feeds containing 1-8 ppm maduramicin are extracted with acetone, the extract is passed through an alumina column, the column is eluted with acetonitrile-water (90 + 10), and the eluate is analyzed for maduramicin by liquid chromatography-AAS after concentration and conversion of maduramicin to the sodium salt. Recoveries of maduramicin averaged 89.5%. Liquid chromatography with AAS detection has been shown to be a sensitive and highly specific technique for the determination of ionophores in general and maduramicin in particular.
Johnson
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American Cyanamid Co., Agricultural Research Center, Princeton, NJ 08540.
D000821:Animal Feed / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D007476:Ionophores / Q000032:analysis*; D007783:Lactones / Q000032:analysis; D007832:Lasalocid / Q000032:analysis; D008985:Monensin / Q000032:analysis; D009550:Nigericin / Q000032:analysis; D013054:Spectrophotometry, Atomic
D007476:Ionophores; D007783:Lactones; C018091:maduramicin; D008985:Monensin; D009550:Nigericin; D007832:Lasalocid