Development and applications of ultrasensitive enzyme immunoassays for antigens and antibodies.
Some enzymes can be measured at lower molar concentrations than the radioisotopes used as labels in radioimmunoassay. Therefore, enzyme immunoassay should be capable of higher sensitivity than radioimmunoassay, if appropriate techniques are used for enzyme-labelling of antibodies and formulation of assay protocols. Techniques required for enzyme immunoassay with attomole sensitivity have been developed, and successfully applied to the measurement of clinically important ligands at levels below those possible using radioimmunoassay. In addition, a novel enzyme immunoassay technique (immune complex transfer enzyme immunoassay technique) is being developed to provide higher sensitivity.
Ishikawa
E
E
Department of Biochemistry, Medical College of Miyazaki, Japan.
Hashida
S
S
Tanaka
K
K
Kohno
T
T
D000906:Antibodies / Q000032:analysis*; D000941:Antigens / Q000032:analysis*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007328:Insulin / Q000097:blood; D008297:Male; D013954:Thyroglobulin / Q000097:blood
D000906:Antibodies; D000941:Antigens; D007328:Insulin; D013954:Thyroglobulin