Kupffer cell number is normal, but their lysozyme content is reduced in alcoholic liver disease.
A quantitative immunohistochemical study of Kupffer cells in liver biopsies from alcoholics was carried out. Two markers were compared, lysozyme and an antimacrophage monoclonal antibody EMB/11. The results showed that there is no significant reduction in Kupffer cell numbers in acute and chronic alcoholic liver disease but that there is a reduction in the number of Kupffer cells staining positively for lysozyme. Thus, those clinical phenomena such as endotoxaemia in alcoholic liver disease are not due to reduction in Kupffer cell number but perhaps to a functional deficit in these cells.
Kelly
P M
PM
University of Oxford, Nuffield Department of Pathology, John Radcliffe Hospital, Headington, U.K.
Heryet
A R
AR
McGee
J O
JO
D000911:Antibodies, Monoclonal / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D001707:Biopsy, Needle; D002452:Cell Count; D006801:Humans; D007150:Immunohistochemistry; D007728:Kupffer Cells / Q000201:enzymology*; D008108:Liver Diseases, Alcoholic / Q000201:enzymology*; D009113:Muramidase / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D009113:Muramidase