[Standardization of plates used in the ELISA reaction].
The results of ELISA reactions made on the entire area of the plates, in the same way in all wells revealed that it is essential to eliminate the marginal rows. Even then there are in some instances deviations from the mean. These deviations frequently follow a loop-sided or dish-like pattern with the minimum in the centre of the plate. The deviations equalled in different wells on average 5-10% (or 20%) of the optic density. Between individual rows there may be a 0.100 difference in optic density, in rare instances as much as 0.300. These differences may influence the results during dilution of sera in a series more detailed than 4n and also when examining a single dilution of sera repeated in an inadequate number of wells.
Frühbauer
Z
Z
Pokorný
J
J
D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation*
Standardnost desticek uzívaných v reakci ELISA.