Does membrane-associated DNA play a role in the establishment of hormonal imprinting in the unicellular Tetrahymena?
Treatment of Tetrahymena with DNase considerably reduced the FITC-insulin binding capacity of the unicellular organism, but a normalization of the binding value followed after 24 h. DNase equally prevented imprinting in Tetrahymena when applied before or after insulin. It appears that membrane-associated DNA plays an important role in the hormone binding of Tetrahymena, and also presumably in the development and/or stabilization of hormonal imprinting.
Csaba
G
G
Department of Biology, Semmelweis University of Medicine, Budapest, Hungary.
Darvas
Z
Z
László
V
V
Kovács
P
P
D000818:Animals; D004247:DNA / Q000502:physiology*; D003851:Deoxyribonucleases; D007328:Insulin / Q000378:metabolism*; D011972:Receptor, Insulin / Q000378:metabolism*; D013768:Tetrahymena / Q000235:genetics* / Q000502:physiology
D007328:Insulin; D004247:DNA; D011972:Receptor, Insulin; D003851:Deoxyribonucleases