Purification and characterization of a major phospholipase A2 from Russell's viper (Vipera russelli) venom.
A major phospholipase A2 (VRV PL-VIIIa) which constitutes 24% of the whole Vipera russelli venom was purified to homogeneity by CM-Sephadex C-25 column chromatography followed by gel filtration on Sephadex G-50. VRV PL-VIIIa is a basic protein with a molecular weight of 11,800 by SDS-PAGE. This enzyme contributes 45% of the total PLA2 activity of the venom, but it is least toxic compared to other purified basic PLA2 enzymes prepared from V. russelli venom. The LD50 value (i.p.) of VRV PL-VIIIa is 5.3 mg/kg body wt. It shows neurotoxic symptoms and damages vital organs such as lung, liver and kidney at LD50 doses. It induces myonecrosis when injected i.m. into the thigh muscle of mice and edema when injected into the foot pads.
Kasturi
S
S
Department of Studies in Biochemistry, Manasagangotri, University of Mysore, India.
Gowda
T V
TV
D000818:Animals; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D066298:In Vitro Techniques; D007525:Isoelectric Focusing; D007928:Lethal Dose 50; D008168:Lung / Q000473:pathology; D051379:Mice; D008970:Molecular Weight; D009132:Muscles / Q000473:pathology; D009336:Necrosis / Q000139:chemically induced; D010740:Phospholipases / Q000302:isolation & purification*; D010741:Phospholipases A / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D054467:Phospholipases A2; D013379:Substrate Specificity; D014757:Viper Venoms / Q000032:analysis*
D014757:Viper Venoms; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2