DNA probes bind non-specifically to eosinophils during in situ hybridization: carbol chromotrope blocks binding to eosinophils but does not inhibit hybridization to specific nucleotide sequences.
Eosinophils were found to bind radiolabelled DNA probes non-specifically during in situ hybridization. Pretreatment of cells with carbol chromotrope blocked non-specific binding without interfering with the recognition of specific nucleotide sequences.
Patterson
S
S
Division of Immunological Medicine, Clinical Research Centre, Harrow, Middlesex, U.K.
Gross
J
J
Webster
A D
AD
D015342:DNA Probes; D004279:DNA, Viral / Q000378:metabolism*; D004804:Eosinophils / Q000378:metabolism*; D006801:Humans; D007202:Indicators and Reagents; D009693:Nucleic Acid Hybridization
D015342:DNA Probes; D004279:DNA, Viral; D007202:Indicators and Reagents