The effect of allopurinol on lysosomal enzyme release.
Values of released enzymes from PMNL under resting conditions and after phagocytic stimulation in non-treated patients with gout were increased in comparison with healthy controls and hyperuricemic patients without joint involvement. Allopurinol, given to gout-patients for 3 weeks, had an inhibitory effect on PMNL lysosomal enzyme release from which was also observable after stimulation by opsonized zymosan. Similar results were obtained in experiments in vitro. The 1 hr preincubation of isolated polymorphonuclear leukocytes with allopurinol in concentrations from 0.01 mM-1 mM resulted in a dose-dependent decrease of enzymes released into extracellular space.
Mikulíková
D
D
Institute of Rheumatic Diseases, Piestany, Czechoslovakia.
Bosmanský
K
K
Bosák
V
V
Ondrasík
M
M
D000328:Adult; D000368:Aged; D000493:Allopurinol / Q000009:adverse effects / Q000627:therapeutic use*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005966:Glucuronidase / Q000378:metabolism*; D006073:Gout / Q000188:drug therapy*; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008247:Lysosomes / Q000187:drug effects*; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000187:drug effects*; D010196:Pancreatic Elastase / Q000378:metabolism*; D010587:Phagocytosis / Q000187:drug effects
D000493:Allopurinol; D007770:L-Lactate Dehydrogenase; D005966:Glucuronidase; D010196:Pancreatic Elastase