title: Mechanisms of increased lower esophageal sphincter pressure following intraduodenal peptone infusion in dogs.
abstract: Peptone perfusion of the excluded duodenum in dogs is associated with an increase in lower esophageal sphincter pressure (LESP). This study investigates the role of cholinergic, adrenergic, and hormonal mediators in the response of the LES to intraduodenal peptone infusion. Adult dogs underwent duodenal exclusion via a Roux-en-Y pylorojejunostomy with formation of a mucocutaneous fistula. Manometric measurements of LESP and radioimmunoassay determinations of gastrin and pancreatic polypeptide (PP) blood levels were made at rest and at 15-min intervals following peptone infusion of the excluded duodenum. In control experiments, peptone infusion resulted in an increase in mean LESP at all time intervals (P less than 0.05). PP blood levels increased significantly, while gastrin levels remained unchanged. Both truncal vagotomy and pretreatment with atropine blocked the changes in LESP. PP release in response to peptone was accentuated in vagotomized dogs, while atropine suppressed the release of PP following peptone infusion. Treatment with 6-hydroxydopamine did not affect the increase in either LESP or PP blood levels observed in controls. Intravenous somatostatin suppressed the release of PP following intraduodenal peptone, but did not block the lower esophageal sphincter response. This data indicates that the increase in LESP seen following intraduodenal peptone infusion is centrally mediated and dependent on vagal innervation and cholinergic neurotransmission.
authors.affiliation: Department of Surgery, University of Iowa, Iowa City 52242.
authors.forename: A D
authors.initials: AD
authors.lastname: Sandler
authors.forename: M R
authors.initials: MR
authors.lastname: Karin
authors.forename: J F
authors.initials: JF
authors.lastname: Schlegel
authors.forename: J W
authors.initials: JW
authors.lastname: Maher
authors.forename: W C
authors.initials: WC
authors.lastname: Coltharp
authors.forename: J E
authors.initials: JE
authors.lastname: McGuigan
mesh_terms: D000818:Animals; D001285:Atropine / Q000494:pharmacology; D004285:Dogs; D004943:Esophagogastric Junction / Q000187:drug effects* / Q000502:physiology; D005755:Gastrins / Q000097:blood*; D010191:Pancreatic Polypeptide / Q000097:blood*; D010461:Peptones / Q000008:administration & dosage / Q000494:pharmacology*; D011292:Premedication; D011312:Pressure; D013997:Time Factors; D015167:Vagotomy, Truncal
chemical_list: D005755:Gastrins; D010461:Peptones; D010191:Pancreatic Polypeptide; D001285:Atropine