title: Carnivora: the primary structure of the giant otter (Pteronura brasiliensis, Mustelidae) hemoglobin.
abstract: The hemoglobin of the Giant Otter (Pteronura brasiliensis, Carnivora) contains only one component. The complete primary structures of the alpha- and beta-chains are presented. The globin chains were separated by high-performance liquid chromatography and the sequences determined by automatic liquid- and gas-phase Edman degradation of the chains and their tryptic peptides. The alpha-chains show 18 and the beta-chains 12 exchanges compared with human alpha- and beta-chains, respectively. In the alpha-chains, two substitutions involve alpha 1/beta 1-contacts and one a heme-contact. In the beta-chains one alpha 1/beta 1-, one alpha 1/beta 2- and one heme-contact are exchanged. The alpha- and beta-chains of the Giant Otter are compared to those of the Common Otter and other Carnivora hemoglobins.
authors.affiliation: Max-Planck-Institut für Biochemie, Abteilung Proteinchemie, Martinsried bei München.
authors.forename: T
authors.initials: T
authors.lastname: Kleinschmidt
authors.forename: G
authors.initials: G
authors.lastname: Braunitzer
authors.forename: H G
authors.initials: HG
authors.lastname: Scheil
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002335:Carnivora / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005914:Globins / Q000032:analysis; D006454:Hemoglobins / Q000032:analysis*; D008969:Molecular Sequence Data; D010041:Otters / Q000378:metabolism*; D010449:Peptide Mapping; D012967:Sodium Dodecyl Sulfate
chemical_list: D006454:Hemoglobins; D012967:Sodium Dodecyl Sulfate; D005914:Globins