[A preliminary study on mechanism of respiratory deficiency of mutagenesis in S. cerevisiae].
The results of ethidium bromide treatment for two different strains of S. cerevisiae were reported here. The recovery of respiratory competence was discovered when cells were treated with EB under non-growing condition. However, this recovery can be inhibited by caffeine. It was not observed when cells were treated with EB under growing condition. Similar results were obtained when cells were treated with EB during their exposure to KCN. The formation of at least one intermediate state [rho-] was proposed. The process of formation of respiratory deficiency could be expressed by rho+ in equilibrium [rho-]----rho-. The different concentrations of sucrose supplemented to the culture of S. cerevisiae resulted in certain influence in the experiment.
Gao
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Liu
J J
JJ
Jin
J L
JL
D004996:Ethidium / Q000633:toxicity; D009153:Mutagens; D009154:Mutation; D010101:Oxygen Consumption / Q000187:drug effects / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics*
D009153:Mutagens; D004996:Ethidium