title: [A method for obtaining a long-lived intestinal tissue culture].
abstract: The interest in the studies of the intestines using the method of tissue organ culture has considerably grown in recent years. It can be explained by the great possibilities of obtaining unique data about the state of intestines in normal and pathological condition, e.g. malabsorption syndrome. The paper deals with the method modified by the authors to obtain long-living (24 hours) intestinal tissue organ culture. The investigations used bioptic sections which were obtained by jejunoscopy with spot biopsy of children suffering from intestinal malabsorption. The viability of the explants was proved by histological and histochemical tests. The promise held by the methods is emphasized.
authors.forename: M B
authors.initials: MB
authors.lastname: Kuberger
authors.forename: D D
authors.initials: DD
authors.lastname: Sviridov
authors.forename: N A
authors.initials: NA
authors.lastname: Izachik
authors.forename: V P
authors.initials: VP
authors.lastname: Nazhimov
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Izachik
mesh_terms: D001706:Biopsy; D002648:Child; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods; D004724:Endoscopy; D006801:Humans; D007583:Jejunum / Q000473:pathology*; D008286:Malabsorption Syndromes / Q000473:pathology; D013997:Time Factors; D014022:Tissue Survival
chemical_list: D003470:Culture Media
vernacular_title: K metodike polucheniia dolgozhivushcheĭ intestinal'noĭ kul'tury tkani.