Nitrogen-15 NMR studies of rat liver in vitro and in vivo.
In the present 2.1 T15N NMR study two different kinds of experiments are presented. In one we show that metabolic reactions of 15N-labelled glycine can be followed in the isolated rat liver. In the second we demonstrate that [15N]glycine can be detected using NMR in vivo. For quantification and identification of glycine and metabolites 15N-isotope analysis (emission spectrometry technique) was used. To our knowledge, this is the first demonstration of 15N NMR for study of the metabolism of 15N-labelled compounds in vivo.
Gründer
W
W
Sektion Physik der Karl-Marx-Universität Leipzig, GDR.
Krumbiegel
P
P
Buchali
K
K
Blesin
H J
HJ
D000818:Animals; D005998:Glycine / Q000378:metabolism*; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D009587:Nitrogen Isotopes; D051381:Rats; D011919:Rats, Inbred Strains
D009587:Nitrogen Isotopes; D005998:Glycine