title: In vitro growth of rat bone marrow BFU-E.
abstract: Pokeweed mitogen-stimulated rat spleen cells were identified as a reliable source of rat burst-promoting activity (PBA), which permitted development of a reproducible assay for rat bone marrow erythroid burst-forming units (BFU-E). Optimum BPA dose, assay time, cell dose, and erythropoietin requirements for rat BFU-E were identified. A serum-free assay and a method for stimulating endogenous bone marrow BPA were developed. Identification of sources of rat BPA and characterization of the rat BFU-E assay makes this species more useful for hematopoietic studies. The similarity of rat BFU-E to human and mouse BFU-E strengthens the validity of rat models for erythropoiesis.
authors.affiliation: Department of Laboratory Hematology, Cleveland Clinic Foundation, Ohio.
authors.forename: R C
authors.initials: RC
authors.lastname: Meagher
authors.forename: S A
authors.initials: SA
authors.lastname: Rothmann
authors.forename: I K
authors.initials: IK
authors.lastname: Paik
authors.forename: P
authors.initials: P
authors.lastname: Paul
mesh_terms: D000818:Animals; D001790:Blood Physiological Phenomena; D001853:Bone Marrow; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003470:Culture Media; D004900:Erythroblasts / Q000187:drug effects / Q000502:physiology*; D004920:Erythropoiesis / Q000187:drug effects; D004921:Erythropoietin / Q000494:pharmacology; D005260:Female; D006801:Humans; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen
chemical_list: D003470:Culture Media; D004921:Erythropoietin