A new species of enkephalin precursor mRNA with a distinct 5'-untranslated region in haploid germ cells.
To elucidate the primary structure of preproenkephalin (A) mRNA expressed by haploid germ cells (round spermatids) in rat testis, we have screened a lambda gt11 cDNA library for preproenkephalin cDNA inserts. The largest cDNA insert contained a protein-coding sequence encoding 269 amino acid residues as well as 327 and 309 bases of the 5'- and 3'-untranslated regions, respectively. The protein-coding region plus 3'-untranslated region of the mRNA was over 99% homologous to that of brain preproenkephalin mRNA, whereas the 5'-untranslated region contained a distinct sequence including a partial sequence of intron A of the preproenkephalin gene [(1984) J. Biol. Chem. 259, 14301-14308; (1984) J. Biol. Chem. 259, 14309-14313]. Northern blot analysis using a 5'-end-specific probe showed that this type of preproenkephalin mRNA exists exclusively in the germ cells.
Yoshikawa
K
K
Department of Molecular Biology, Psychiatric Research Institute of Tokyo, Japan.
Maruyama
K
K
Aizawa
T
T
Yamamoto
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry; D003062:Codon; D004247:DNA / Q000235:genetics; D004745:Enkephalins / Q000235:genetics*; D006238:Haploidy; D007438:Introns; D008297:Male; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D012689:Sequence Homology, Nucleic Acid; D013087:Spermatids / Q000032:analysis*; D014018:Tissue Distribution
D003062:Codon; D004745:Enkephalins; D011498:Protein Precursors; D012333:RNA, Messenger; D004247:DNA; C033660:preproenkephalin