title: Determination of partial amino acid sequence of lipoate acetyltransferase of rat pyruvate dehydrogenase complex.
abstract: The partial amino acid sequence of rat lipoate acetyltransferase was determined using the intact protein and the peptides derived from a digest with Achromobacter protease I. The results showed the amino-terminal sequence of the mature enzyme to be (N) Ser-Leu-Pro-Pro-His-Gln-Lys-Val-Pro-Leu-Pro-Ser- Leu-Ser-Pro-Thr-Met-Gln-Ala-Gly-Thr-Ile-Ala-Arg-Trp-Glu-Lys. In addition, the sequences of two possible lipoyl-binding sites in the subunit, which are very similar to each other, were established.
authors.affiliation: Department of Biology, Kanoya National Institute of Fitness and Sports, Kagoshima, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Matuda
authors.forename: K
authors.initials: K
authors.lastname: Nakano
authors.forename: T
authors.initials: T
authors.lastname: Saheki
authors.forename: R
authors.initials: R
authors.lastname: Oyama
authors.forename: M
authors.initials: M
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Titani
mesh_terms: D000123:Acetyltransferases / Q000032:analysis / Q000378:metabolism; D000421:Alcaligenes / Q000201:enzymology; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D051047:Dihydrolipoyllysine-Residue Acetyltransferase; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000378:metabolism; D011768:Pyruvate Dehydrogenase Complex; D051381:Rats; D012689:Sequence Homology, Nucleic Acid
chemical_list: D000596:Amino Acids; D011768:Pyruvate Dehydrogenase Complex; D000123:Acetyltransferases; D051047:Dihydrolipoyllysine-Residue Acetyltransferase; D010447:Peptide Hydrolases