[Immunoaffinity purification of alpha 1-protease inhibitor from human plasma].
A single-step procedure is described for isolation of alpha 1-inhibitor of proteases (alpha 1-IP) from human blood plasma using affinity chromatography on immunosorbent containing monoclonal antibodies against alpha 1-IP. Activity of the alpha 1-IP preparation, estimated by means of specific trypsin inhibition, was increased 61.1-fold after the chromatography, with a yield of the end product 20%. The preparation of alpha 1-IP obtained exhibited electrophoretic and immunochemical homogeneity.
Podiarene
S M
SM
Letskene
M N
MN
Mauritsas
M M
MM
Planchiunene
R R
RR
D000911:Antibodies, Monoclonal; D002846:Chromatography, Affinity; D006801:Humans; D007122:Immunoelectrophoresis; D007163:Immunosorbent Techniques; D000515:alpha 1-Antitrypsin / Q000276:immunology / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D000515:alpha 1-Antitrypsin
Immunoaffinnaia ochistka alpha 1-ingibitora proteaz iz plazmy krovi cheloveka.