Controlling the growth rate of Saccharomyces cerevisiae cells using the glucose analogue D-glucosamine.
By using competition between glucose and its analogue D-glucosamine, we have produced a system in which it is possible to vary the steady-state growth rate of populations of Saccharomyces cerevisiae cells without otherwise altering the composition of the medium or significantly affecting catabolite repression. We demonstrate that D-glucosamine inhibits the accumulation of glucose derived label and the phosphorylation of glucose by hexokinase (EC 2.7.1.1).
McGoldrick
E M
EM
School of Biological Sciences, University of Bath, UK.
Wheals
A E
AE
D002455:Cell Division / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005944:Glucosamine / Q000494:pharmacology*; D005947:Glucose / Q000493:pharmacokinetics; D006593:Hexokinase / Q000037:antagonists & inhibitors; D012441:Saccharomyces cerevisiae / Q000254:growth & development* / Q000378:metabolism
D006593:Hexokinase; D005947:Glucose; D005944:Glucosamine