Novel molecular analogues of phosphatidylcholines in a lipid extract from bovine brain: 1-long-chain acyl-2-short-chain acyl-sn-glycero-3-phosphocholines.
A vasodepressor phospholipid fraction named Depressor-IA from a bovine brain lipid extract was analyzed by capillary gas-liquid chromatography-mass spectrometry as tert-butyldimethylsilyl derivatives after hydrolysis with phospholipase C. Results show that Depressor-IA is a mixture of 3 platelet-activating factors and 17 1-acyl analogues. Three platelet-activating factors having sn-1-O-hexadecyl, octadecyl, and octadecenyl groups were suggested to account for the hypotensive activity of Depressor-IA, although the total amount of 1-acyl analogues of platelet-activating factor was much more than that of platelet-activating factor in the purified Depressor-IA. 1-Long-chain acyl-2-short-chain acyl-glycero-3-phosphocholines identified in Depressor-IA included novel molecular analogues having sn-2-propionyl, acryloyl, butyryl, valeryl, caproyl, and hepatonoyl groups.
Tokumura
A
A
Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
Takauchi
K
K
Asai
T
T
Kamiyasu
K
K
Ogawa
T
T
Tsukatani
H
H
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D008401:Gas Chromatography-Mass Spectrometry; D008055:Lipids / Q000032:analysis*; D010713:Phosphatidylcholines / Q000302:isolation & purification*; D010972:Platelet Activating Factor / Q000032:analysis* / Q000138:chemical synthesis
D008055:Lipids; D010713:Phosphatidylcholines; D010972:Platelet Activating Factor