Immunodiagnosis of human anisakiasis by use of larval excretory-secretory antigen.
L3 larvae of Anisakis simplex, isolated from freshly caught herrings were cultured in Medium 199 at different temperatures to obtain excretory-secretory (ES) antigen. Larvae have been cultured in vitro for a period of 11 months. A crude extract of A. simplex larvae was compared with ES antigen obtained under 4 different culture conditions for the determination of IgG and IgE antibodies by ELISA and a modified RAST, respectively. 1 serum from a patient with histopathologically proved anisakiasis and 4 samples from persons with presumptive clinical disease were positive in the ELISA. Two sera were positive in the RAST. A combination of both tests is suggested for the serodiagnosis of human anisakiasis.
Poggensee
U
U
Landesinstitut für Tropenmedizin, Berlin West.
Schommer
G
G
Jansen-Rosseck
R
R
Feldmeier
H
H
D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis*; D000947:Antigens, Helminth / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005399:Fishes; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007814:Larva / Q000276:immunology; D009348:Nematoda / Q000276:immunology*; D009349:Nematode Infections / Q000175:diagnosis* / Q000276:immunology; D011237:Predictive Value of Tests; D011852:Radioallergosorbent Test
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G; D007073:Immunoglobulin E