Monitoring the transport and phosphorylation of 2-deoxy-D-glucose in tumor cells in vivo and in vitro by 13C nuclear magnetic resonance spectroscopy.
We describe the use of 2-deoxy-D-[6-13C]glucose to follow simultaneously, by 13C NMR, both transport and phosphorylation to its 6-phosphate form, in MCF-7 breast cancer cells in vitro and in vivo in subcutaneous tumors in nude mice.
Navon
G
G
Medicine Branch, National Cancer Institute, Bethesda, MD 20892.
Lyon
R C
RC
Kaplan
O
O
Cohen
J S
JS
D000818:Animals; D001692:Biological Transport; D001943:Breast Neoplasms / Q000378:metabolism*; D003837:Deoxy Sugars / Q000378:metabolism*; D003847:Deoxyglucose / Q000378:metabolism*; D019298:Glucose-6-Phosphate / Q000031:analogs & derivatives*; D005958:Glucosephosphates / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000378:metabolism; D010766:Phosphorylation; D014407:Tumor Cells, Cultured
D003837:Deoxy Sugars; D005958:Glucosephosphates; C015785:2-deoxyglucose-6-phosphate; D019298:Glucose-6-Phosphate; D003847:Deoxyglucose