A mitogenic factor from rat muscle.
A growth-promoting polypeptide has been purified about 1950-fold from rat abdominal muscle by gel chromatography, isoelectric-focusing (IEF) and reverse phase high pressure liquid chromatographic (RP-HPLC) techniques. This factor is an acidic protein with an isoelectric point (pI) of 3.4 and a mol. wt of 12 kDa when run on SDS-polyacrylamide gels under reducing conditions. It is acid and heat-stable but is sensitive to reducing agent and protease action. It stimulates [3H]thymidine incorporation in NRK-49F and NIH 3T3 cells and induces soft agar colony formation of NIH 3T3 cells. The muscle-derived mitogen appears to differ from other known growth factors (GFs) in its physico-chemical properties.
Rao
L S
LS
Biological Chemistry Division, Cancer Research Institute, Tata Memorial Centre, Parel, Bombay, India.
Rao
S G
SG
Banerji
A P
AP
D000818:Animals; D001667:Binding, Competitive; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004261:DNA Replication / Q000187:drug effects; D066246:ErbB Receptors / Q000187:drug effects / Q000378:metabolism; D005260:Female; D007525:Isoelectric Focusing; D008934:Mitogens / Q000302:isolation & purification* / Q000494:pharmacology; D008970:Molecular Weight; D009132:Muscles / Q000032:analysis*; D011506:Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D008934:Mitogens; D011506:Proteins; D066246:ErbB Receptors