title: Demonstration of spontaneous XX/XY chimerism by DNA fingerprinting.
abstract: The M13 bacteriophage probe, which makes possible the establishment of DNA fingerprints, was used to study a phenotypically normal woman with a 46XY karyotype and her twin brother. Identical fingerprints and positive hybridization with a series of Y-specific probes were obtained on blood samples from the siblings. DNA from a skin biopsy of the woman yielded a clearly different pattern and displayed no Y-specific hybridization, indicating that she is a spontaneous chimera. This study illustrates the use of DNA fingerprinting as a powerful and simple aid to the diagnosis of natural chimerism.
authors.affiliation: Department of Immunology, Cliniques Universitaires Erasme, Brussels, Belgium.
authors.forename: C M
authors.initials: CM
authors.lastname: Farber
authors.forename: M
authors.initials: M
authors.lastname: Georges
authors.forename: G
authors.initials: G
authors.lastname: De Bock
authors.forename: A
authors.initials: A
authors.lastname: Verhest
authors.forename: P
authors.initials: P
authors.lastname: Simon
authors.forename: M
authors.initials: M
authors.lastname: Verschraegen-Spae
authors.forename: G
authors.initials: G
authors.lastname: Vassart
mesh_terms: D000328:Adult; D001435:Bacteriophages; D002678:Chimera; D005260:Female; D006801:Humans; D007621:Karyotyping / Q000379:methods; D007962:Leukocytes / Q000032:analysis; D008297:Male; D009710:Nucleotide Mapping; D012730:Sex Chromosomes; D014427:Twins