Distribution of Cu, Zn, and Fe in the soluble fraction of the kidney in normal, copper-poisoned, and thiomolybdate-treated sheep.
Twenty-seven sheep were used in two experiments to study the distribution of copper (Cu), zinc (Zn), and iron (Fe) in the kidney cytosol of control, Cu-loaded, and thiomolybdate (TM)-treated sheep. A comparison was made of the patterns of distribution on Sephadex G-75 of Cu, Zn, and Fe from the cytosol of fresh and frozen kidneys and after thawing of frozen cytosol. In both Cu-dosed and TM-treated sheep, the absolute level of Cu increased in the cytosol, but the percent of Cu decreased. The percent recovery of Cu from the frozen kidney was comparable to that from fresh kidney, but the extraction of Cu from the supernatant of frozen cytosol was approximately 10% less. This was due to a loss of Cu in a precipitate that formed when the frozen cytosol was thawed. Most of the Cu in the cytosol from the kidney of Cu-loaded sheep was in a metallothionein (MT)-like protein fraction and was trichloroacetic acid (TCA) soluble. In contrast, that from the cytosol of TM-treated sheep was mostly in a high molecular weight fraction that was TCA insoluble. The chromatograms obtained from cytosol derived from frozen kidneys, or cytosol that had itself been frozen, contained a similar distribution of Cu, Zn, and Fe, but the peak heights were lower in the latter samples.
Gooneratne
S R
SR
Department of Animal and Poultry Science, University of Saskatchewan, Canada.
Gawthorne
J M
JM
Howell
J M
JM
D000818:Animals; D002850:Chromatography, Gel; D003300:Copper / Q000378:metabolism / Q000506:poisoning*; D003600:Cytosol / Q000378:metabolism; D005615:Freezing; D007501:Iron / Q000378:metabolism; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D008982:Molybdenum / Q000494:pharmacology*; D012756:Sheep; D015032:Zinc / Q000378:metabolism
D003300:Copper; D008982:Molybdenum; C020809:tetrathiomolybdate; D007501:Iron; D015032:Zinc