title: In-vitro processing of sperm with autoantibodies and in-vitro fertilization results.
abstract: In-vitro fertilization and embryo transfer were carried out in twenty infertile couples with significant levels of anti-sperm antibodies on the ejaculated spermatozoa. Over 21 cycles (15 couples), 95 mature oocytes were collected and inseminated with spermatozoa obtained by swim-up migration after rapid dilution and washing of the ejaculates. An overall fertilization rate of 38.9% was obtained with these post-migration (PM) preparations. When greater than 70% of the inseminated spermatozoa were covered with both IgG and IgA antibodies, only 14% of the 43 oocytes were fertilized. A higher fertilization rate was obtained with the PM preparations containing less than 70% of spermatozoa coated with one or both classes of antibodies. Under these conditions 60% of the 52 oocytes were fertilized. Fertility rate correlated better with IgG than with IgA antibody levels. In order to decrease the proportion of antibody-coated spermatozoa in the inseminated populations, washed spermatozoa were immuno-adsorbed on Mage's plates before swim-up migration. Over 11 cycles (10 couples) 52 mature oocytes were inseminated with these post-migration immuno-depleted sperm preparations (PMP) containing less than 65% of antibody-coated spermatozoa: 31% of the oocytes were fertilized. This rate compares favourably to IVF results obtained with the PM preparations with greater than 70% of spermatozoa coated with both classes of antibodies. In five couples who had different IVF attempts with the two sperm preparations, the immuno-depletion resulted in a slight increase in the fertilization rates: 10% for the PM preparations versus 26% for the PMP preparations. Sperm binding to the zona pellucida was decreased in the majority of unsuccessful attempts.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Laboratoire d'Histologie Embryologie et Cytogénétique, Centre Hospitalier de Bicêtre, France.
authors.forename: M
authors.initials: M
authors.lastname: de Almeida
authors.forename: I
authors.initials: I
authors.lastname: Gazagne
authors.forename: C
authors.initials: C
authors.lastname: Jeulin
authors.forename: M
authors.initials: M
authors.lastname: Herry
authors.forename: J
authors.initials: J
authors.lastname: Belaisch-Allart
authors.forename: R
authors.initials: R
authors.lastname: Frydman
authors.forename: P
authors.initials: P
authors.lastname: Jouannet
authors.forename: J
authors.initials: J
authors.lastname: Testart
mesh_terms: D000328:Adult; D001323:Autoantibodies / Q000032:analysis*; D004624:Embryo Transfer; D005307:Fertilization in Vitro; D006801:Humans; D007248:Infertility, Male / Q000276:immunology* / Q000628:therapy; D008297:Male; D013094:Spermatozoa / Q000276:immunology*
chemical_list: D001323:Autoantibodies