Glycine and other amino compounds prevent chromatin precipitation at physiological ionic strength.
Glycine, proline and taurine, when present in the range 0.1-0.60 M, inhibit chromatin precipitation by sodium chloride. Histone gel electrophoresis revealed that the linker histones H1 and H5 were largely depleted from the supernatant chromatin fraction at 0.2 M NaCl, while this depletion was absent in the presence of glycine. These observations are discussed in relation with the various factors which may be involved in the precipitation process.
Buche
A
A
Laboratoire de Chimie Macromoléculaire et Chimie Physique, Université de Liège, Belgium.
Ouassaidi
A
A
Hacha
R
R
Delpire
E
E
Gilles
R
R
Houssier
C
C
D000310:Adrenal Gland Neoplasms; D000818:Animals; D011232:Chemical Precipitation; D002645:Chickens; D002843:Chromatin / Q000187:drug effects* / Q000378:metabolism; D002942:Circular Dichroism; D004247:DNA / Q000378:metabolism; D004912:Erythrocytes / Q000032:analysis; D005998:Glycine / Q000494:pharmacology*; D006657:Histones / Q000378:metabolism; D006358:Hot Temperature; D009994:Osmolar Concentration; D010673:Pheochromocytoma; D011392:Proline / Q000494:pharmacology*; D011489:Protein Denaturation; D051381:Rats; D012965:Sodium Chloride / Q000494:pharmacology*; D013654:Taurine / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D002843:Chromatin; D006657:Histones; D013654:Taurine; D012965:Sodium Chloride; D004247:DNA; D011392:Proline; D005998:Glycine