Application of solid-phase antibodies to radioimmunoassay. Improved performance of Dynospheres following affinity purification of antisera.
Affinity purification of antibody before covalent binding to pre-activated micro-particles (Dynospheres) was necessary to overcome the reduced coupling capacity evident with low titre antisera, particularly antihapten sera. Optimally prepared Dynospheres anti-thyroxine provided a radioimmunoassay with good performance and economical usage of microparticles. Assay sensitivity was less than 10 nmol/l while precision across the clinically relevant dose range (50-150 nmol/l) was 3% CV using 125 micrograms particles per assay tube.
McConway
M G
MG
Institute of Biochemistry, Royal Infirmary, Glasgow, UK.
Chapman
R S
RS
D000937:Antigen-Antibody Reactions; D002846:Chromatography, Affinity; D007074:Immunoglobulin G / Q000276:immunology / Q000302:isolation & purification*; D011863:Radioimmunoassay / Q000379:methods*; D013974:Thyroxine / Q000276:immunology*
D007074:Immunoglobulin G; D013974:Thyroxine