Enhancement of kainate-gated currents in retinal horizontal cells by cyclic AMP-dependent protein kinase.
Dopamine, acting via cyclic adenosine 3':5'-monophosphate (cAMP), has been shown to enhance a kainate-gated ionic conductance in white perch retinal horizontal cells in vitro. To determine whether this effect involves stimulation of a protein kinase, kainate-gated currents were observed in cultured horizontal cells that were dialyzed with the catalytic subunit of cAMP-dependent protein kinase. Intracellular application of catalytic subunit or cAMP, but not heat-inactivated catalytic subunit, caused significant enhancement of the kainate-evoked currents. These results suggest that kainate-gated channels in horizontal cells may be modified by a phosphorylation event.
Liman
E R
ER
Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA 02138.
Knapp
A G
AG
Dowling
J E
JE
D000818:Animals; D002478:Cells, Cultured; D007608:Kainic Acid / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects; D010471:Perches / Q000502:physiology*; D010473:Perciformes / Q000502:physiology*; D011494:Protein Kinases / Q000378:metabolism*; D012160:Retina / Q000166:cytology / Q000187:drug effects / Q000502:physiology*
D011494:Protein Kinases; D007608:Kainic Acid