Search for nicotinic acetylcholine receptors on human leukocytes: absence of alpha-bungarotoxin binding in studies of healthy individuals and myasthenia gravis patients.
To investigate whether nicotinic acetylcholine receptors might be present on blood mononuclear cells we studied the binding [125I]alpha-bungarotoxin to mononuclear cells from three normal controls and seven myasthenia gravis patients. The medulloblastoma cell line, TE671, which expresses a functional nicotinic acetylcholine receptor having pharmacological properties similar to that of skeletal muscle receptor, was used as a positive control for alpha-bungarotoxin binding. None of the mononuclear cell samples studied exhibited specific binding of alpha-bungarotoxin.
Kaufman
R L
RL
Department of Pathology, University of British Columbia, Vancouver, Canada.
Oger
J
J
D002038:Bungarotoxins / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008527:Medulloblastoma / Q000378:metabolism; D009157:Myasthenia Gravis / Q000378:metabolism*; D011950:Receptors, Cholinergic / Q000032:analysis*; D011978:Receptors, Nicotinic / Q000032:analysis*; D064569:alpha7 Nicotinic Acetylcholine Receptor
D002038:Bungarotoxins; C579167:Chrna7 protein, human; D011950:Receptors, Cholinergic; D011978:Receptors, Nicotinic; D064569:alpha7 Nicotinic Acetylcholine Receptor