Production of intracellular alpha-glucosidase in brewery waste by Saccharomyces carlsbergenesis.
Saccharomyces carlsbergensis strain ATCC 9080 was grown on acid hydrolyzed brewery waste. The substrate contains 22 mg/ml of total carbohydrates of which approximately 60% represents reducing sugars such as maltose and glucose. Optimal conditions for substrate hydrolysis were 1.5% w/v sulfuric acid, 20 minutes and temperature of 110 degrees C. Maximum enzyme production was obtained in a medium containing 0.05% w/v yeast extract at 35 degrees C and at an initial pH of 6. Addition of nitrogen sources was unnecessary. Optimal conditions for alpha-glucosidase extraction from yeast included pH 7.5, 30 degrees C, and 10 minutes of sonication. Maximum hydrolysis of PNPG occurred at pH 7.5, temperature between 30 degrees C and 25 minutes of incubation. Michaelis-Menten constants and Vmax were 1 mM, 0.56 mol/min/mg of protein on P-nitrophenly-alpha-D-glucophyra-noside and 5 mV, 5 mol/min/mg of protein on maltose respectively.
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D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D012440:Saccharomyces / Q000378:metabolism*; D000520:alpha-Glucosidases / Q000096:biosynthesis* / Q000378:metabolism
D003470:Culture Media; D007220:Industrial Waste; D000520:alpha-Glucosidases