Treatment of fetal bovine serum with activated charcoal enhances spontaneous differentiation of murine erythroleukemia cells.
We established a spontaneous differentiation system for murine erythroleukemia cells, MELC-DS19, with high inducibility (80%). The spontaneous differentiation reflected the cells' loss of proliferative capacity. However, this phenomenon was only observed using a subclone, i.e. DS19-sc4, with an inducer-mediated commitment to terminal cell differentiation that was not suppressed by dexamethasone. Essential aspects of the system are the low initial cell density (less than 10(4)/ml) and the use of fetal bovine serum (FBS) treated with activated charcoal instead of ordinary FBS. This system is useful for studying the molecular basis of the spontaneous differentiation of MELC as well as the erythropoiesis regulating factor of sera.
Murate
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First Department of Internal Medicine, Nagoya University School of Medicine, Japan.
Kaneda
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D000818:Animals; D001769:Blood; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D002606:Charcoal / Q000494:pharmacology; D003470:Culture Media; D003907:Dexamethasone / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D004915:Leukemia, Erythroblastic, Acute; D051379:Mice; D008970:Molecular Weight; D011073:Polyamines / Q000494:pharmacology
D003470:Culture Media; D011073:Polyamines; D002606:Charcoal; D003907:Dexamethasone