[Immune electron microscope determination of the localization of tryptophanyl-tRNA-synthetase in bacteria and higher eukaryotes].
Localization of tryptophanyl-tRNA synthetase (TRS) was studied on ultrathin (UT) sections of Escherichia coli cells and of rat fibroblasts fixed with glutaraldehyde and embedded in "Lowicryl K4M" resin at -35 degrees C. The UT sections were treated with the complexes of monoclonal and/or polyclonal antibodies against TRS with colloidal gold 15 and 8 nm in size. In both types of the cells cytoplasm was the most intensely labelled. In fibroblast cytoplasm, zones with a greater amount of ribosomes were mainly labelled, the gold particles being found over both the cysternae of granular endoplasmic reticulum and the areas of localization of free ribosomes. In the zones of microfilament localization TRS was not detected. A great amount of TRS was found in mitochondria and in the fibroblast nuclei. In the latter case, the label was concentrated over the diffuse chromatin localization regions, a minimal binding being observed over compact chromatin. The number of particles observed over diffuse chromatin equals to 50-80% against the label in fibroblast cytoplasm. In contrast, the label used to be absent over the E. coli nucleoid. The presence of TRS in the fibroblast nucleus may evidence in favour of a possible regulatory role of TRS in eukaryots.
Popenko
V I
VI
Cherni
N E
NE
Beresten'
S F
SF
Zargarova
T A
TA
Favorova
O O
OO
D000604:Amino Acyl-tRNA Synthetases / Q000032:analysis*; D000818:Animals; D000911:Antibodies, Monoclonal; D002467:Cell Nucleus / Q000201:enzymology / Q000648:ultrastructure; D004926:Escherichia coli / Q000201:enzymology* / Q000648:ultrastructure; D005347:Fibroblasts / Q000201:enzymology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D051381:Rats; D013045:Species Specificity; D014369:Tryptophan-tRNA Ligase / Q000032:analysis*
D000911:Antibodies, Monoclonal; D000604:Amino Acyl-tRNA Synthetases; D014369:Tryptophan-tRNA Ligase
Immuno-élektronno-mikroskopicheskoe opredelenie lokalizatsii triptofanil-tRNK-sintetazy v kletkakh bakteriĭ i vysshikh éukariot.