title: Antibodies to nystatin demonstrate polyene sterol specificity and allow immunolabeling of sterols in Saccharomyces cerevisiae.
abstract: Polyclonal antibodies elicited by injection into rabbits of a nystatin-bovine serum albumin conjugate were reactive with both nystatin and amphotericin B. Upon labeling of polyene-treated Saccharomyces cerevisiae sterol auxotrophs grown on various sterols, nystatin reacted specifically with ergosterol, while amphotericin B did not react preferentially with ergosterol, cholesterol, or cholestanol. Time course labeling experiments demonstrated the rate of ergosterol transport into cholesterol-grown cells.
authors.affiliation: Department of Microbiology, North Carolina State University, Raleigh 27695-7615.
authors.forename: H M
authors.initials: HM
authors.lastname: Walker-Caprioglio
authors.forename: J M
authors.initials: JM
authors.lastname: MacKenzie
authors.forename: L W
authors.initials: LW
authors.lastname: Parks
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D001711:Biotransformation; D004797:Enzyme-Linked Immunosorbent Assay; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D009761:Nystatin / Q000276:immunology*; D011090:Polyenes / Q000276:immunology; D011817:Rabbits; D012441:Saccharomyces cerevisiae / Q000032:analysis* / Q000276:immunology; D013056:Spectrophotometry, Ultraviolet; D013261:Sterols / Q000032:analysis / Q000276:immunology*
chemical_list: D011090:Polyenes; D013261:Sterols; D009761:Nystatin