title: The response of the double strand-specific nuclease V1 to Y-base removal in yeast tRNA(Phe).
abstract: The patterns of limited hydrolysis of yeast tRNA(Phe) and tRNA(-YPhe) by double strand-specific ribonuclease V1 show some differences in cleavage of both the acceptor stem and the anticodon stem. These regions are considerably better substrates for RNase V1 in tRNA(-YPhe) than in tRNA(Phe). The results are interpreted in favour of conformational changes taking place in yeast tRNA(Phe) upon the Y-base1 removal.
authors.affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, Poland.
authors.forename: T
authors.initials: T
authors.lastname: Marciniec
authors.forename: J
authors.initials: J
authors.lastname: Ciesiołka
authors.forename: W
authors.initials: W
authors.lastname: Krzyzosiak
mesh_terms: D000818:Animals; D000926:Anticodon; D002417:Cattle; D004722:Endoribonucleases; D006868:Hydrolysis; D009690:Nucleic Acid Conformation; D012345:RNA, Transfer, Amino Acid-Specific; D012360:RNA, Transfer, Phe; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D000926:Anticodon; D012345:RNA, Transfer, Amino Acid-Specific; D012360:RNA, Transfer, Phe; D004722:Endoribonucleases; C032825:ribonuclease V(1)