The use of long-term bone marrow cultures (LTBMC) to detect hematotoxic side effects of purging methods.
With a view to the establishment of purging methods it is necessary to investigate complete-remission bone marrow as the real target of purging, rather than bone marrow from healthy donors. The results of LTBMC are superior to those of GM-CFC where the hematopoietic recovery of bone marrow is concerned. One-stage LTBMC after bone marrow manipulations may reflect mixed hematopoietic/stromal effects. The use of two-stage LTBMC allows the evaluation of the stem cell recovery without the possible influence of a damaged microenvironment.
Schulze
E
E
Department of Internal Medicine, Karl-Marx-University, Leipzig, German Democratic Republic.
Helbig
W
W
Hofmann
U
U
D001853:Bone Marrow / Q000187:drug effects* / Q000378:metabolism; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000378:metabolism; D005047:Etoposide / Q000494:pharmacology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D013997:Time Factors
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D005047:Etoposide; D016178:Granulocyte-Macrophage Colony-Stimulating Factor