Nascent very low density lipoproteins from rat hepatocytic Golgi fractions are enriched in phosphatidylethanolamine.
The phospholipid composition of nascent very low density lipoproteins (VLDL) of rat hepatocytic Golgi fractions differs greatly from that of plasma VLDL. The phospholipids of nascent VLDL contain about four times more phosphatidylethanolamine (PE) than plasma VLDL, whereas plasma VLDL contain considerably more sphingomyelin. Thus, the ratio of PE to sphingomyelin differs by a factor of about 12 between nascent Golgi VLDL and circulating plasma VLDL. It is evident from these data that the PE/sphingomyelin ratio of VLDL can be used to estimate endosomal contamination of hepatocytic Golgi fractions.
Hamilton
R L
RL
Cardiovascular Research Institute, University of California, San Francisco 94143-0130.
Fielding
P E
PE
D000818:Animals; D006056:Golgi Apparatus / Q000032:analysis*; D008079:Lipoproteins, VLDL / Q000032:analysis* / Q000097:blood; D008099:Liver / Q000648:ultrastructure*; D008244:Lysophosphatidylcholines / Q000032:analysis / Q000097:blood; D008297:Male; D010713:Phosphatidylcholines / Q000032:analysis / Q000097:blood; D010714:Phosphatidylethanolamines / Q000032:analysis* / Q000097:blood; D010716:Phosphatidylinositols / Q000032:analysis / Q000097:blood; D010718:Phosphatidylserines / Q000032:analysis / Q000097:blood; D051381:Rats; D013109:Sphingomyelins / Q000032:analysis
D008079:Lipoproteins, VLDL; D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010716:Phosphatidylinositols; D010718:Phosphatidylserines; D013109:Sphingomyelins