title: [Possible activation of human prorenin by contact with the cell membrane].
abstract: Semipurified human kidney prorenin was exposed in vitro to a mixture of lipids mimicking the composition of the inner leaflet of the cell membrane, in the presence of semipurified human angiotensinogen at a concentration of 1/4 Km. Prorenin was activated in a time-dependent manner over a period of 60 min. This lipid-dependent activation was completely reversed thereafter. Pre-incubation with anti-renin serum completely prevented this activated prorenin-dependent generation of angiotensin I. Our data suggest that human prorenin can be reversibly activated by contact with the cell membrane.
authors.forename: C
authors.initials: C
authors.lastname: Troffa
authors.forename: G
authors.initials: G
authors.lastname: Tonolo
authors.forename: P
authors.initials: P
authors.lastname: Manunta
authors.forename: A
authors.initials: A
authors.lastname: Pazzola
authors.forename: M G
authors.initials: MG
authors.lastname: Melis
authors.forename: F
authors.initials: F
authors.lastname: Pala
authors.forename: A
authors.initials: A
authors.lastname: Soro
authors.forename: P
authors.initials: P
authors.lastname: Madeddu
authors.forename: N
authors.initials: N
authors.lastname: Glorioso
mesh_terms: D000803:Angiotensin I / Q000096:biosynthesis*; D002462:Cell Membrane / Q000378:metabolism*; D004792:Enzyme Precursors / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D050356:Lipid Metabolism; D012083:Renin / Q000378:metabolism*
chemical_list: D004792:Enzyme Precursors; D000803:Angiotensin I; D012083:Renin
vernacular_title: Possibile attivazione di contatto della prorenina umana con la membrana cellulare.