title: Mirror densitometry for higher sensitivity and resolution.
abstract: In order to increase the pathway of the light inside a gel (or autoradiogram) during scanning, it is placed on top of a mirror and the densitometry is performed in a vertical reflection mode. As the light passes the gel a second time after being reflected by the mirror, the absorbance is nearly doubled. This increase in absorbance results in higher sensitivity and resolution.
authors.affiliation: Max-Planck-Institut für experimentelle Medizin, Forschungstelle Neurochemie, Göttingen, Federal Republic of Germany.
authors.forename: D
authors.initials: D
authors.lastname: Taube
authors.forename: V
authors.initials: V
authors.lastname: Neuhoff
mesh_terms: D001345:Autoradiography; D003720:Densitometry / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D005069:Evaluation Studies as Topic; D008029:Lighting; D055095:Optics and Photonics; D011506:Proteins / Q000302:isolation & purification
chemical_list: D011506:Proteins