High-performance liquid chromatographic assay of nesosteine in human plasma and urine.
A high-performance liquid chromatographic method for the quantitation of the mucoactive drug sodium 2-(3-thiazolidinylcarbonyl)benzoate in plasma and urine was developed, involving liquid-liquid extraction under acidic conditions and reversed-phase chromatography using ultraviolet detection at 210 or 235 nm. The extraction efficiency, linearity, limit of detection, precision and accuracy were determined. Products of the main biotransformation of the drug, involving hydrolysis of the amidic bond, do not interfere. The method is selective, precise, reproducible and applicable to studies of the pharmacokinetic behaviour of the drug in humans. Pharmacokinetic parameters derived from six healthy subjects following acute intravenous administration of the drug (150 mg) are presented.
Girometta
M A
MA
Analytical Chemistry Department, Camillo Corvi SpA, Piacenza, Italy.
Loschi
L
L
Ventura
P
P
D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D008297:Male; D013056:Spectrophotometry, Ultraviolet; D013844:Thiazoles / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine
D013844:Thiazoles; C049549:nesosteine