Sensitivity of rat and mouse peripheral blood lymphocytes to BaP adduction and SCE formation.
Both mice and rats were injected i.p. with doses of benzo[a]pyrene (BaP) ranging from 10 to 100 mg/kg to compare species sensitivity to and the relationship between sister chromatid exchange (SCE) induction and DNA adduct formation. Twenty-four hours after injection, blood was removed by cardiac puncture and the peripheral blood lymphocytes (PBLs) were analyzed for both DNA adduct formation by 32P-postlabeling and SCE induction following lymphocyte culture. BaP induced similar, but not identical, SCE dose-response curves for each species. After BaP administration, the major DNA adduct, N2-[10 beta-(7 beta,8 alpha,9 alpha-trihydroxy-7,8,9,10- tetrahydrobenzo[a]pyrene)yl]deoxyguanosine (BPDEI-dGuo), was approximately 10-fold more prevalent in the PBLs of the mouse than those of the rat. Thus, for equivalent amounts of BPDEI-dGuo, a greater number of SCEs are induced in the rat than the mouse.
Kligerman
A D
AD
Genetic Toxicology Division, Health Effects Research Laboratory, Environmental Protection Agency, Research Triangle Park, NC 27711.
Nesnow
S
S
Erexson
G L
GL
Earley
K
K
Gupta
R C
RC
D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000473:pathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D001564:Benzo(a)pyrene; D004247:DNA