title: Physico-chemical characteristics of superoxide dismutase in Ascaris suum.
abstract: 1. Three SOD isoenzymes obtained from purified extracts of Ascaris suum were characterized. 2. The physico-chemical characteristics studied were: optimum pH, methods of preservation of enzymatic activity, molecular weight, and the u.v. and visible light absorption spectra. 3. The optimum pH for the Cu, Zn SOD I and II was 10.2 and 10.1 for the Mn SOD. 4. The extracts retained their levels of activity longer at -70 degrees C, and after lyophilization. The Mn SOD was more labile than the Cu, Zn SOD I and II. 5. The molecular weights obtained by filtration through Sephadex G-75 were: 73,000 for Mn SOD; 42,600 for Cu, Zn SOD I; and 39,800 for the Cu, Zn SOD II. 6. Both the u.v. and visible light spectra were similar to other dismutases from other sources.
authors.affiliation: Instituto Lopez-Neyra de Parasitologia Sección de Bioquimica, Granada, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Sanchez-Moreno
authors.forename: M A
authors.initials: MA
authors.lastname: Garcia-Ruiz
authors.forename: A
authors.initials: A
authors.lastname: Sanchez-Navas
authors.forename: M
authors.initials: M
authors.lastname: Monteoliva
mesh_terms: D000818:Animals; D001200:Ascaris / Q000032:analysis*; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet; D013482:Superoxide Dismutase / Q000032:analysis*
chemical_list: D013482:Superoxide Dismutase