End-label fingerprintings show that an N-terminal segment of depactin participates in interaction with actin.
A 1:1 complex of actin and depactin, an actin-depolymerizing protein isolated from starfish oocytes [Mabuchi, I. (1983) J. Cell Biol. 97, 1612-1621], was cross-linked with 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide (EDC) to introduce covalent bonds at their contact site. Locations of cross-linking sites were identified along the depactin sequence by the end-label fingerprinting, which employed site-directed antibodies against the N- and C-termini of depactin as end labels. Mappings with these end labels have revealed that the N-terminal segment of depactin (residues 1-20) contains sites in contact with the N- and C-terminal segments of actin, both of which participate in interaction with depactin [Sutoh, K., & Mabuchi, I. (1986) Biochemistry 25, 6186-6192].
Sutoh
K
K
Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan.
Mabuchi
I
I
D051339:Actin Depolymerizing Factors; D000199:Actins / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D003432:Cross-Linking Reagents; D003488:Cyanogen Bromide; D051345:Destrin; D005022:Ethyldimethylaminopropyl Carbodiimide; D066298:In Vitro Techniques; D008840:Microfilament Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D012697:Serine Endopeptidases
D051339:Actin Depolymerizing Factors; D000199:Actins; D003432:Cross-Linking Reagents; D051345:Destrin; D008840:Microfilament Proteins; D012697:Serine Endopeptidases; C036813:lysyl endopeptidase; D003488:Cyanogen Bromide; D005022:Ethyldimethylaminopropyl Carbodiimide