title: Ion-exchange chromatographic determination of hypoglycin A in canned ackee fruit.
abstract: An ion-exchange chromatographic method was developed to determine hypoglycin A (HG-A) levels in canned ackee fruit by using an amino acid analyzer. HG-A was extracted by homogenizing the sample in 80% alcohol. An isocratic buffer system, consisting of 30% sodium citrate buffer (pH 3.15) and 70% sodium chloride-sodium acetate buffer (pH 7.40) was used to obtain baseline separation between HG-A and the other amino acids. The system can detect HG-A levels as low as 4.8 micrograms/mL. HG-A levels in the edible portion of fruit in 6 cans ranged from 11.0 to 66.5 mg HG-A/can. Recoveries by standard addition averaged 102.5%.
authors.affiliation: Food and Drug Administration, Atlanta, GA 30309.
authors.forename: G W
authors.initials: GW
authors.lastname: Chase
authors.forename: W O
authors.initials: WO
authors.lastname: Landen
authors.forename: L T
authors.initials: LT
authors.lastname: Gelbaum
authors.forename: A G
authors.initials: AG
authors.lastname: Soliman
mesh_terms: D000596:Amino Acids / Q000032:analysis; D002852:Chromatography, Ion Exchange; D003521:Cyclopropanes / Q000032:analysis*; D005519:Food Preservation; D005638:Fruit / Q000032:analysis*; D007005:Hypoglycins / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy
chemical_list: D000596:Amino Acids; D003521:Cyclopropanes; D007005:Hypoglycins