title: A spectrophotometric method for determining the stream sample core diameter of a flow cytometer.
abstract: A simple, rapid, and accurate method is described for the determination of flow cytometer stream sample core diameters. A precisely formulated solution of potassium dichromate is introduced as a sample, the stream is collected, and its absorbance is measured using a spectrophotometer. This permits the calculation of the factor by which the original solution was diluted and, given the stream diameter, the sample core diameter.
authors.affiliation: Department of Pathology, University of New Mexico School of Medicine, Albuquerque 87131.
authors.forename: P
authors.initials: P
authors.lastname: Blume
mesh_terms: D005434:Flow Cytometry / Q000295:instrumentation*; D013053:Spectrophotometry / Q000379:methods*