The response of the double strand-specific nuclease V1 to Y-base removal in yeast tRNA(Phe).
The patterns of limited hydrolysis of yeast tRNA(Phe) and tRNA(-YPhe) by double strand-specific ribonuclease V1 show some differences in cleavage of both the acceptor stem and the anticodon stem. These regions are considerably better substrates for RNase V1 in tRNA(-YPhe) than in tRNA(Phe). The results are interpreted in favour of conformational changes taking place in yeast tRNA(Phe) upon the Y-base1 removal.
Marciniec
T
T
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, Poland.
Ciesiołka
J
J
Krzyzosiak
W
W
D000818:Animals; D000926:Anticodon; D002417:Cattle; D004722:Endoribonucleases; D006868:Hydrolysis; D009690:Nucleic Acid Conformation; D012345:RNA, Transfer, Amino Acid-Specific; D012360:RNA, Transfer, Phe; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D000926:Anticodon; D012345:RNA, Transfer, Amino Acid-Specific; D012360:RNA, Transfer, Phe; D004722:Endoribonucleases; C032825:ribonuclease V(1)