title: Cytogenetic analysis of in vitro fertilization (IVF) failures.
abstract: Cytogenetic studies were carried out on 150 oocytes obtained in a human in vitro fertilization (IVF) program. Although all cells lacked signs of fertilization at light microscopy, 46 (30.7%) appeared to show cytological evidence of fertilization. At least one-third of these cells (with development arrested before first cleavage) had chromosomal aberrations. An aneuploidy rate of 35% was found in unfertilized oocytes. The results of this study explain some of the fertilization failures and of the failures of postfertilization development.
authors.affiliation: Department of Genetics and Cell Biology, University of Limburg, Maastricht, The Netherlands.
authors.forename: M H
authors.initials: MH
authors.lastname: Pieters
authors.forename: J P
authors.initials: JP
authors.lastname: Geraedts
authors.forename: J C
authors.initials: JC
authors.lastname: Dumoulin
authors.forename: J L
authors.initials: JL
authors.lastname: Evers
authors.forename: M
authors.initials: M
authors.lastname: Bras
authors.forename: F H
authors.initials: FH
authors.lastname: Kornips
authors.forename: P P
authors.initials: PP
authors.lastname: Menheere
mesh_terms: D000782:Aneuploidy; D002869:Chromosome Aberrations; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D009865:Oocytes / Q000648:ultrastructure*; D011123:Polyploidy