Membrane effects of the polyene antibiotic amphotericin B and of some of its derivatives on lymphocytes.
Amphotericin B (AmB) exhibits immunomodulating properties in mice. In vitro studies on lymphocytes, in relation with these properties, are reported here with AmB and two of its derivatives: the N-Fructosyl (N-Fru AmB) and the N-thiopropionyl (AmBSH) derivatives. Interactions of these molecules with thymocytes, a sensitive cell type, demonstrated that the extent of binding is not a toxicity parameter. In contrast, membrane fluidity changes have been observed and appeared to be related to toxicity. Experiments performed with normal B lymphocytes have shown that Amphotericin B derivatives were more potent polyclonal B cell activators than the parent compound. To go further in the understanding of these events, we have investigated in a B cell line WEHI 231, the changes in intracellular Ca2+ and membrane potential induced by AmB and AmBSH. The two polyenes were shown to induce membrane depolarization but no intracellular Ca2+ increase.
Henry-Toulmé
N
N
Laboratoire de Physique et Chimie Biomoléculaire, CNRS USA 198, Université Paris 6, France.
Sarthou
P
P
Seman
M
M
Bolard
J
J
D000666:Amphotericin B / Q000494:pharmacology*; D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects*; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects*; D005453:Fluorescence; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects
D000666:Amphotericin B