title: Experimental polyvalent ISCOMs subunit vaccine induces antibodies that neutralize human and bovine respiratory syncytial virus.
abstract: The purpose of the present study was to evaluate experimentally, in guinea-pigs, the immunogenicity of respiratory syncytial (RS) virus subunit vaccines. Immunostimulating complexes (ISCOMs), made from the surface proteins of both human (Long) and bovine (A-51908) RS strains adsorbed to the adjuvant Quil A, were assayed for their capacity to induce neutralizing antibodies, in comparison to experimental live virus vaccines. Serums from animals vaccinated with either the human or bovine RS subunit vaccines were equally efficient in neutralizing human or bovine RS virus. ISCOMs prepared with bovine RS virus proteins were significantly (p less than 0.05%) more efficient than their human counterpart, in inducing neutralizing antibodies, suggesting their greater potential as a subunit vaccine.
authors.affiliation: Centre de Recherche en Virologie, Institut Armand-Frappier, Université du Québec, Ville de Laval, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Trudel
authors.forename: F
authors.initials: F
authors.lastname: Nadon
authors.forename: C
authors.initials: C
authors.lastname: Séguin
authors.forename: C
authors.initials: C
authors.lastname: Simard
authors.forename: G
authors.initials: G
authors.lastname: Lussier
mesh_terms: D000276:Adjuvants, Immunologic; D000327:Adsorption; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000917:Antibody Formation; D000936:Antigen-Antibody Complex / Q000276:immunology; D002417:Cattle; D006168:Guinea Pigs; D006801:Humans; D008854:Microscopy, Electron; D009687:Nuclear Proteins / Q000378:metabolism; D062605:Quillaja Saponins; D012136:Respiratory Syncytial Viruses / Q000276:immunology*; D012503:Saponins / Q000493:pharmacokinetics; D014760:Viral Fusion Proteins / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D000936:Antigen-Antibody Complex; D009687:Nuclear Proteins; D062605:Quillaja Saponins; D012503:Saponins; D014760:Viral Fusion Proteins; D014765:Viral Vaccines; C046386:Quil A