A novel transcription unit within the exon sequence of the rat insulin like growth factor II gene.
We identified a transcription unit within a single exon of the rat insulin like growth factor II (rIGFII) gene by a combination of Northern blotting, S1 nuclease mapping and primer extension analyses. Among multiple mRNA products of rIGFII, the 1.8kb transcript has a discrete 5' terminus and is encoded exclusively in a single 3'-extreme exon with no trace of the coding region. The upstream region of the 5' terminus contains a completely matching GC-rich 16bp palindrome and several sequence motifs highly homologous to the consensus sequences of binding sites for transcriptional regulatory proteins AP2, H2TF1 and NF-kappa B.
Matsuguchi
T
T
Department of Biochemistry, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.
Takahashi
K
K
Ueno
T
T
Endo
H
H
Yamamoto
M
M
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D005091:Exons; D005796:Genes; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D013002:Somatomedins / Q000235:genetics*; D014158:Transcription, Genetic
D012333:RNA, Messenger; D013002:Somatomedins; D007335:Insulin-Like Growth Factor II