Accumulation of sulphite by Saccharomyces cerevisiae and Zygosaccharomyces bailii as affected by phospholipid fatty-acyl unsaturation and chain length.
Analyses were made of the fatty-acyl composition of phospholipids from each of two strains of Saccharomyces cerevisiae and Zygosaccharomyces bailii grown aerobically. Residues of C16:0, C16:1 and C18:1 predominated in phospholipids from strains of the first yeast, while phospholipids from Z. bailii contained mainly C16:0, C18:1 and C18:2 residues. S. cerevisiae NCYC 431, grown anaerobically in media supplemented with ergosterol and C14:1, C16:1, C18:1, C18:2, C18:3 or C20:1 fatty acids, contained phospholipids enriched with residues of the exogenously provided acid, to a greater extent with shorter chain than longer chain acids. A plot of the permeability coefficient for sulphite, derived from Woolf-Eadie plots, against the degree of unsaturation in phospholipids (expressed as delta mol-1 value) showed that the coefficient was greater the lower the degree of unsaturation in the phospholipids. A plot of the permeability coefficient against values for the mean fatty-acyl chain length divided by the delta mol-1 value, which is an approximation of the cross-section surface area of a phospholipid molecule, showed that the permeability coefficient tended to increase the greater the surface-area value.
Pilkington
B J
BJ
Zymology Laboratory, School of Biological Sciences, Bath University, Avon, UK.
Rose
A H
AH
D002463:Cell Membrane Permeability / Q000502:physiology; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D005227:Fatty Acids / Q000494:pharmacology; D010743:Phospholipids / Q000032:analysis*; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000378:metabolism*; D004718:Saccharomycetales / Q000378:metabolism*; D013447:Sulfites / Q000493:pharmacokinetics*
D005227:Fatty Acids; D010743:Phospholipids; D013447:Sulfites