Use of palladium(II) chloride as a colour-forming reagent for the determination of N-acetyl-L-cysteine in water and fluimukan injections.
The formation of the complex between N-acetyl-L-cysteine and palladium(II) chloride in Britton-Robinson buffer solution in the pH range 2.08-8.00 was studied. The optimum conditions for this reaction were ascertained and a spectrophotometric method was developed for the determination of N-acetyl-L-cysteine in the concentration range 4.0-65.3 micrograms ml-1, using PdCl2 as the reagent. The detection limit was 1.63 micrograms ml-1 and the relative standard deviation varied from 0.63 to 1.92%. The method was applied to the determination of N-acetyl-L-cysteine in water and in injection solutions.
Jovanović
T S
TS
Stanković
B S
BS
D000111:Acetylcysteine / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007267:Injections; D010165:Palladium; D013056:Spectrophotometry, Ultraviolet; D014867:Water / Q000032:analysis*
D007202:Indicators and Reagents; D014867:Water; D010165:Palladium; C008756:palladium chloride; D000111:Acetylcysteine