Enzyme linked immunosorbent assay: determination of anti-adenovirus antibodies in an infant population.
In order to define an accurate assay for anti-adenovirus antibody detection, a recently developed ELISA was compared with IFA and CF. On 58 sera, the ELISA was more sensitive than both CF and IFA, which showed relative sensitivities of 63% and 94%, respectively. It was not possible to determine the exact specificity of the tests because of the lack of a gold standard. Furthermore, the ELISA was used to define the prevalence of adenovirus antibodies in 116 infants between 1 and 24 months old (mean 7.28). The data showed that maternal antibodies waned by the age of 5 to 6 months and that more than 80% of the children had been infected by adenoviruses by the age of 10 months.
Weinberg
A
A
Fink
M C
MC
Takimoto
S
S
Ishida
M A
MA
Souza
M C
MC
D000256:Adenoviridae / Q000276:immunology*; D000258:Adenovirus Infections, Human / Q000175:diagnosis; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D000914:Antibodies, Viral / Q000032:analysis*; D003168:Complement Fixation Tests; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D007112:Immunity, Maternally-Acquired; D007075:Immunoglobulin M / Q000032:analysis*; D007223:Infant
D000888:Antibodies, Anti-Idiotypic; D000914:Antibodies, Viral; D007075:Immunoglobulin M