title: Alpha 1-acid glycoprotein high-performance liquid chromatography column (EnantioPAC) as a screening tool for protein binding.
abstract: An attempt was made to correlate retention behavior on a high-performance liquid chromatographic (HPLC) system employing an immobilized alpha 1-acid glycoprotein (AAG) column with AAG binding behavior for various compounds. Protein binding was assessed by propranolol displacement studies in an equilibrium dialysis system using isolated AAG. HPLC retention behavior poorly correlated with propranolol displacement from AAG. This system is not suitable for use as a screening tool for AAG affinity.
authors.affiliation: College of Pharmacy, University of Kentucky, Lexington 40536.
authors.forename: R C
authors.initials: RC
authors.lastname: Jewell
authors.forename: K L
authors.initials: KL
authors.lastname: Brouwer
authors.forename: P J
authors.initials: PJ
authors.lastname: McNamara
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003956:Dialysis; D006863:Hydrogen-Ion Concentration; D009961:Orosomucoid; D011433:Propranolol / Q000032:analysis; D011485:Protein Binding; D013237:Stereoisomerism
chemical_list: D009961:Orosomucoid; D011433:Propranolol