Fibronectin network recovery in confluent PtK2 cells after acrylamide treatment.
Confluent PtK2 cells 4 hr treated with 5 mM acrylamide were FN-detected by indirect immunofluorescence. The initial fibrillar-FN network was replaced by an alveolar-type network located at the cell-cell contacts areas in the form of a thick frame with a lace-like appearance. Afterwards, acrylamide removal was obtained by several washes with fresh FCS-free culture medium. Then, PtK2 cells were returned to the incubator for 20 hr. Cell recovery was indicated by reversion of the initial fibrillar-FN network. These data show that FN reversion was possible without any changes in shape and cytoplasmic organization of non-motile growing cells.
Denèfle
J P
JP
Centre de Biologie Cellulaire CNRS, Ivry-sur-Seine, France.
Zhu
Q L
QL
D020106:Acrylamide; D000178:Acrylamides / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D005353:Fibronectins / Q000187:drug effects* / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D008856:Microscopy, Fluorescence
D000178:Acrylamides; D005353:Fibronectins; D020106:Acrylamide