Compositional analysis of the three main gangliosides from adult human myometrium by a rapid capillary gas chromatographic method.
A new capillary GC method is described for the compositional analysis of the three main gangliosides isolated from adult human myometrium. The sample was subjected to methanolysis, acetylation and trimethylsilylation which allows all the constituents to be analyzed simultaneously. The predominant ganglioside was found to be GD3, with GM3 and GT1b the next most abundant.
Zhu
Z M
ZM
Department of Biochemistry, Dalian Medical College, China.
Li
R X
RX
Cui
Z C
ZC
Zhou
L M
LM
Huang
M X
MX
D000328:Adult; D002241:Carbohydrates / Q000032:analysis; D002849:Chromatography, Gas; D005260:Female; D005679:G(M3) Ganglioside / Q000032:analysis; D005732:Gangliosides / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D009215:Myometrium / Q000032:analysis*; D014599:Uterus / Q000032:analysis
D002241:Carbohydrates; D005679:G(M3) Ganglioside; D005732:Gangliosides; C016481:trisialoganglioside GT1; C026226:ganglioside, GD3