Solubilization and characterization of a ouabain-sensitive protein from transverse tubule membrane-junctional sarcoplasmic reticulum complexes (TTM-JSR) in cat cardiac muscle.
A new glycoprotein of 31,500 dalton, which has a high affinity for ouabain, and is independent of (Na+-K+)-ATPase, was solubilized from transverse tubule membrane and junctional sarcoplasmic reticulum complexes (TTM-JSR) of cat cardiac muscle. This protein could be extracted only in small amounts from sarcolemma-plasma membrane (SLM-PL) fragments, suggesting that it indeed originates from the TTM-JSR.
Fujino
S
S
Department of Pharmacology, Hokkaido Institute of Pharmaceutical Sciences, Otaru, Japan.
Satoh
K
K
Bando
T
T
Kurokawa
T
T
Nakai
T
T
Takashima
K
K
Fujino
M
M
D000818:Animals; D002415:Cats; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D006352:Heart Ventricles / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000302:isolation & purification / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism* / Q000648:ultrastructure; D008970:Molecular Weight; D009206:Myocardium / Q000378:metabolism* / Q000648:ultrastructure; D010042:Ouabain / Q000494:pharmacology*; D012519:Sarcoplasmic Reticulum / Q000378:metabolism* / Q000648:ultrastructure; D012995:Solubility
D008562:Membrane Glycoproteins; D010042:Ouabain