Glucocorticoid receptors in alveolar macrophages: methodological aspects of the determination of the number of glucocorticoid receptors per cell.
Among patients with asthma or chronic obstructive pulmonary diseases the response to glucocorticoids varies considerably. To investigate the possible relationship between clinical response and number of glucocorticoid receptors in alveolar macrophages or their KD value a micro receptor assay was developed. Assay conditions were adjusted because of receptor occupancy by endogenous or therapeutically used glucocorticoids and high aspecific binding.
van Hal
P T
PT
Department of Pulmonary Medicine, Erasmus University, Rotterdam, The Netherlands.
Mulder
E
E
Hoogsteden
H C
HC
Hilvering
C
C
Benner
R
R
D002460:Cell Line; D006801:Humans; D008264:Macrophages / Q000032:analysis*; D011650:Pulmonary Alveoli / Q000032:analysis*; D011965:Receptors, Glucocorticoid / Q000032:analysis*; D014407:Tumor Cells, Cultured / Q000032:analysis
D011965:Receptors, Glucocorticoid