title: Determination of triacylglycerol lipase activity using carbon-13-labeled triacylglycerols and nuclear magnetic resonance spectroscopy: evidence that hepatic lipase hydrolyzes medium-chain triacylglycerols.
abstract: Triacylglycerol lipase activity was studied using glycerol [1-13C]trioctanoate mixed with postheparin rat plasma. 13C NMR spectroscopy demonstrated triacylglycerol hydrolysis into free fatty acids with no difference at the 1,3 or 2 glycerol positions. There was no inhibition by high sodium concentration, consistent with lipase of hepatic origin.
authors.affiliation: Department of Pediatrics, University of California, Davis 95616.
authors.forename: R J
authors.initials: RJ
authors.lastname: Dimand
authors.forename: E M
authors.initials: EM
authors.lastname: Bradbury
authors.forename: K L
authors.initials: KL
authors.lastname: Cox
mesh_terms: D000818:Animals; D002247:Carbon Isotopes; D005230:Fatty Acids, Nonesterified / Q000378:metabolism*; D006493:Heparin / Q000494:pharmacology; D008049:Lipase / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D009682:Magnetic Resonance Spectroscopy; D051381:Rats; D011919:Rats, Inbred Strains; D012965:Sodium Chloride / Q000494:pharmacology; D014280:Triglycerides / Q000378:metabolism
chemical_list: D002247:Carbon Isotopes; D005230:Fatty Acids, Nonesterified; D014280:Triglycerides; D012965:Sodium Chloride; D006493:Heparin; D008049:Lipase