title: Effect of neonatal estrogenization on testosterone metabolism in the prostate and in the epididymis of the rat.
abstract: The present experiments were performed in order to analyze whether the administration of estrogens (single injection of 500 micrograms of estradiol benzoate s.c.) to neonatal male rats might modify the weight of the ventral prostate and the epididymis as well as the metabolism of testosterone in these two organs. The metabolism of testosterone was evaluated in vitro using 14C-radiolabelled testosterone as the substrate. The metabolites dihydrotestosterone (DHT), 5 alpha-androstane-3 alpha, 17 beta-diol (3 alpha-diol), 5 alpha-androstane-3 beta,17 beta-diol (3 beta-diol), androstenedione, 5 alpha-androstane-3,17-dione (5-A-dione) and 3 alpha-hydroxy-5 alpha-androstane-17-one (androsterone) were quantified. After neonatal estrogen administration animals were killed on days 22 and 90 of age. The following changes were observed: (1) the body weight, the weight of the testes and of the ventral prostate were lower than in controls on both day 22 and 90; (2) the weight of the epididymides was higher than in controls on day 22 and lower on day 90; (3) in the ventral prostate the in vitro formation of DHT was lower and that of the diols was higher than in control tissue on day 22 of age; (4) the in vitro formation of alpha-reduced metabolites of the 17-keto series (5 alpha-A-dione + androsterone) was higher in ventral prostate of treated animals than in that of controls on day 22; (5) in treated animals, no formation of DHT in the caput epididymis was observed at day 22. On the contrary, at the same age the formation of androstenedione was higher than in controls; on day 90 of age the formation of DHT, androstenedione and the 5 alpha-reduced metabolites of the 17-keto series was identical in caput epididymis of the treated animals and of the controls, while the formation of the diols was higher in the treated than in the controls. The data indicate that neonatal estrogenization may induce important changes in testosterone metabolism in the prostates and in the epididymides of the rat.
authors.affiliation: Department of Endocrinology, University of Milan, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Pinilla
authors.forename: M
authors.initials: M
authors.lastname: Cocconi
authors.forename: S
authors.initials: S
authors.lastname: Zoppi
authors.forename: L
authors.initials: L
authors.lastname: Martini
mesh_terms: D000375:Aging; D015113:Androstane-3,17-diol / Q000378:metabolism; D000735:Androstenedione / Q000378:metabolism; D000738:Androsterone / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D001835:Body Weight / Q000187:drug effects; D013196:Dihydrotestosterone / Q000378:metabolism; D004822:Epididymis / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology*; D005043:Etiocholanolone / Q000031:analogs & derivatives / Q000378:metabolism; D008297:Male; D009929:Organ Size / Q000187:drug effects; D011467:Prostate / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013237:Stereoisomerism; D013737:Testis / Q000254:growth & development; D013739:Testosterone / Q000378:metabolism*
chemical_list: D013196:Dihydrotestosterone; D015113:Androstane-3,17-diol; D013739:Testosterone; D000735:Androstenedione; D004958:Estradiol; C006576:androstane-3,17-dione; D005043:Etiocholanolone; D000738:Androsterone