title: High-performance liquid chromatography separation of nikkomycins X and Z.
abstract: A reversed-phase, C-18 HPLC method for separation, with baseline resolution, of the chitin synthase inhibitors nikkomycin X and Z is described. This permits, for the first time, satisfactory identification of nikkomycin X and Z contained in a mixture. The use of 30 mM ammonium formate (pH 4.7) containing the ion-pair agent heptanesulfonic acid (1 mM) was critical for the successful separation of these fungicides.
authors.affiliation: Southern Regional Research Laboratory, USDA, New Orleans, Louisiana 70124.
authors.forename: P
authors.initials: P
authors.lastname: Engel
authors.forename: H C
authors.initials: HC
authors.lastname: Dischinger
authors.forename: A H
authors.initials: AH
authors.lastname: Ullah
mesh_terms: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology; D000935:Antifungal Agents / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology; D001681:Biological Assay; D002176:Candida albicans / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D015394:Molecular Structure; D013302:Streptomyces / Q000032:analysis*
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; C011952:nikkomycin