Superoxide dismutase induces differentiation of Friend erythroleukemia cells.
Friend erythroleukemia cells (FELC) served as a model system for cell differentiation because these cells can be triggered to differentiate by a variety of chemical agents. Treatment with the classical inducer of differentiation, hexamethylene bisacetamide (HMBA), stimulated superoxide dismutase (SOD) activity, which increased in parallel with HMBA-induced differentiation. Furthermore, FELC were shown to differentiate in response to the addition of liposomes containing SOD. Oxidative treatment with liposomes containing D-amino acid oxidase or xanthine oxidase, cumene peroxide, or potassium superoxide also induced differentiation, whereas antioxidants such as alpha-tocopherol, butylated hydroxytoluene, or beta-carotene did not induce differentiation. Also, HMBA induction of differentiation was suppressed by treatment with antioxidants.
Beckman
B S
BS
Department of Pharmacology, Tulane University School of Medicine, New Orleans, Louisiana 70112.
Balin
A K
AK
Allen
R G
RG
D000081:Acetamides / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects*; D008081:Liposomes / Q000494:pharmacology; D010084:Oxidation-Reduction / Q000187:drug effects; D013482:Superoxide Dismutase / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000081:Acetamides; D000975:Antioxidants; D008081:Liposomes; D013482:Superoxide Dismutase; C014026:hexamethylene bisacetamide