CD spectra of isoionic DNA solutions.
The ion-exchange transition of Na-DNA----H-DNA in concentrated salt-free solutions is accompanied by strong variations in CD spectra. The rotational force of the negative band magnitude of delta epsilon 249 decreases when going to H-DNA by about 4 times, and the value of delta epsilon 279, by 1.2 times. These changes are irreversible to a considerable extent, which is evident because the spectra of Na-DNA obtained by neutralizing isoionic H-DNA solutions with NaOH or by the ion-exchange method significantly differ from those of Na-DNA taken by dissolving solid Na-DNA in deionized water. It has been shown that additions of NaCl to an isoionic solution of DNA leads to variations of spectra, typical for deprotonation processes as well as for an increase in DNA hydration.
Davydova
O V
OV
Kuznetsov
I A
IA
Florentiev
V L
VL
D000818:Animals; D002645:Chickens; D002942:Circular Dichroism; D004247:DNA / Q000097:blood; D004912:Erythrocytes; D009690:Nucleic Acid Conformation; D009994:Osmolar Concentration
D004247:DNA