Delta-sleep-inducing peptide (DSIP) stimulates the release of immunoreactive Met-enkephalin from rat lower brainstem slices in vitro.
We studied whether delta-sleep-inducing peptide (DSIP) acted on opioid receptor directly or indirectly. DSIP did not have binding activity to any subtype of opioid receptors. DSIP at doses of 1 pM-1 nM significantly stimulated the release of immunoreactive Met-enkephalin (iME) from superfused slices of the rat lower brainstem. The DSIP-induced release of iME was calcium-dependent. These results show that DSIP acts on opioid receptor indirectly by stimulating the release of iME in producing antinociceptive effects.
Nakamura
A
A
Department of Pharmacology, Faculty of Pharmacy, Fukuyama University, Japan.
Nakashima
M
M
Sakai
K
K
Niwa
M
M
Nozaki
M
M
Shiomi
H
H
D000818:Animals; D001667:Binding, Competitive; D003701:Delta Sleep-Inducing Peptide / Q000494:pharmacology*; D004174:Diprenorphine / Q000378:metabolism; D004723:Endorphins / Q000378:metabolism; D004744:Enkephalin, Methionine / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D008526:Medulla Oblongata / Q000187:drug effects / Q000378:metabolism*; D011149:Pons / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D004723:Endorphins; D004174:Diprenorphine; D004744:Enkephalin, Methionine; D003701:Delta Sleep-Inducing Peptide