The radiosensitivity of kidney colony-forming cells: a short-term assay in situ in the mouse.
A short-term colony assay for renal tubule epithelium has been developed. Uranyl nitrate (UN) is a heavy metal nephrotoxin that induces acute tubule necrosis followed by a large compensatory increase in the rate of cell proliferation in the nephron. UN was used to precipitate latent damage following renal irradiation. Using a subcapsular colony count at 14 days after unilateral irradiation, a single-dose cell survival curve was obtained with a D0 of 4.2 +/- 0.3 Gy. High-dose irradiation of an exteriorized kidney resulted in a survival curve which was biphasic, with a plateau in survival between 18 and 40 Gy. Subtraction of this plateau level from all the survival data gave D0 values of 2.5 +/- 0.2 Gy (data analyzed between 7.5 and 16 Gy) or 2.0 +/- 0.2 Gy (over range 12-16 Gy). The D0 value obtained at 20 months after bilateral (or unilateral) kidney irradiation, without the use of UN, was 2.9 +/- 1.1 Gy (over range 10-14 Gy).
Ewen
C
C
Department of Radiobiology, Paterson Institute for Cancer Research, Christie Hospital, Manchester, United Kingdom.
Hendry
J H
JH
D003114:Colony-Forming Units Assay; D005260:Female; D007668:Kidney / Q000187:drug effects / Q000528:radiation effects*; D007683:Kidney Tubular Necrosis, Acute / Q000139:chemically induced / Q000473:pathology; D008297:Male; D011836:Radiation Tolerance; D013234:Stem Cells / Q000528:radiation effects*; D014502:Uranyl Nitrate / Q000633:toxicity
D014502:Uranyl Nitrate