Modulation of catecholamine metabolism in synaptosomes by a neuroregulatory factor from mammalian brain.
Incubation of synaptosomes from rat brain with bovine brain extract caused inhibition of oxidative deamination of dopamine, decreased formation of 3,4-dihydroxyphenylacetic acid, increased formation of norepinephrine and its N-methyl derivatives and increased release of catecholamines. Omission of Ca2+ from the extrasynaptosomal medium completely blocked the brain extract induced release of [3H]catecholamines and decreased, by about 80%, the effect on changes in catecholamine metabolism. Chromatography of the brain extract on Sephadex-G25 columns resulted in an active compound eluting at the position expected for compounds with molecular weights in the region of 1500 to 2500 Da.
Pastuszko
A
A
Department of Biochemistry and Biophysics, Medical School, University of Pennsylvania, Philadelphia 19104.
Yee
D K
DK
Wilson
D F
DF
D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002395:Catecholamines / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism; D008297:Male; D008970:Molecular Weight; D009638:Norepinephrine / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism*; D014020:Tissue Extracts / Q000032:analysis / Q000494:pharmacology*
D002395:Catecholamines; D014020:Tissue Extracts; D015102:3,4-Dihydroxyphenylacetic Acid; D004298:Dopamine; D009638:Norepinephrine