title: Structural organization of upstream exons and distribution of transcription start sites in the chicken c-myb gene.
abstract: We mapped and sequenced three upstream exons of the chicken c-myb gene and the regions flanking the first coding exon. We found multiple potential binding sites for transcription factors in the 5'-noncoding region, a T-rich stretch of 78 base pairs (bp) (68% T) in the first intron, and four fairly long open reading frames in the antisense direction of the first coding exon and its flanking regions. Three major transcription start sites, contained within a single 11-bp region, were identified by S1 nuclease analysis and primer extension. A sequence comparison of the avian and murine c-myb genes revealed a highly conserved sequence of 124 bp in the 5'-noncoding region. Its location between the putative transcription factor binding sites and the major transcription start sites suggests that it may play an important regulatory role in c-myb expression.
authors.affiliation: Graduate Program in Molecular Biology, Sloan-Kettering Institute for Cancer Research, New York, New York.
authors.forename: S L
authors.initials: SL
authors.lastname: Hahn
authors.forename: M
authors.initials: M
authors.lastname: Hahn
authors.forename: W S
authors.initials: WS
authors.lastname: Hayward
mesh_terms: D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000235:genetics*; D004247:DNA / Q000235:genetics; D005091:Exons; D008969:Molecular Sequence Data; D011519:Proto-Oncogenes; D015183:Restriction Mapping; D013045:Species Specificity; D014158:Transcription, Genetic
chemical_list: D004247:DNA