Isolation of phenol-degrading Bacillus stearothermophilus and partial characterization of the phenol hydroxylase.
Bacillus stearothermophilus BR219, isolated from river sediment, degraded phenol at levels to 15 mM at a rate of 0.85 mumol/h (4 x 10(6) cells). The solubilized phenol hydroxylase was NADH dependent, exhibited a 55 degrees C temperature optimum for activity, and was not inhibited by 0.5 mM phenol.
Gurujeyalakshmi
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Department of Microbiology and Public Health, Michigan State University, East Lansing 48824-1101.
Oriel
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P
D001411:Geobacillus stearothermophilus / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification*; D007700:Kinetics; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D010636:Phenols / Q000378:metabolism; D012988:Soil Microbiology
D010636:Phenols; D006899:Mixed Function Oxygenases; C022336:phenol 2-monooxygenase