Optimization of the histochemical demonstration of DNA using 3-hydroxy-2-naphthoic acid hydrazide and fast blue B.
Previous methods for the histochemical demonstration of DNA were optimized. p-Toluene sulfonic acid as catalyst for hydrazone formation between the aldehydes generated after Feulgen hydrolysis and 3-hydroxy-2-naphthoic acid hydrazide (NAH) was used instead of acetic acid. Modifications of the conditions of the coupling reaction with Fast Blue B reduced the background staining. The optimized histochemical staining method for DNA (NAH-FB-DNA staining) can be performed easily and reproducibly. Without prior Feulgen hydrolysis the optimized method can also be used for the histochemical demonstration of reactive carbonyls undissolved under the given histochemical conditions.
Nöhammer
G
G
Institut für Biochemie, Karl-Franzens-Universität Graz, Austria.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA / Q000032:analysis*; D003979:Diazonium Compounds; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007202:Indicators and Reagents; D007700:Kinetics; D008099:Liver / Q000166:cytology; D009284:Naphthols; D051381:Rats; D014407:Tumor Cells, Cultured
D003979:Diazonium Compounds; D007202:Indicators and Reagents; D009284:Naphthols; C020747:Fast Blue B; C010589:3-hydroxy naphthoic acid hydrazide; D004247:DNA