Peptide sequencing by partial methanolysis and fast atom bombardment mass spectrometry.
The possibility of obtaining sequence information on peptides by partial methanolysis with 5 N HCl in dry methanol and subsequent fast atom bombardment (FAB) of the resulting mixture was investigated. This procedure was tested using four peptides of different size and amino acid composition. The results obtained demonstrate that this approach is effective in producing FAB spectra containing more sequence information than the spectra of the untreated peptides. For the compounds investigated the spectra contain enough information to unequivocally reassemble the original sequence.
Foti
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Dipartimento di Scienze Chimiche dell' Università di Catania, Italy.
Saletti
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D000595:Amino Acid Sequence; D006868:Hydrolysis; D013058:Mass Spectrometry; D000432:Methanol; D010455:Peptides / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D010455:Peptides; D000432:Methanol