title: A simplified method for the preparation of WGA-Sepharose 4B and its use in the purification of MN blood group antigens.
abstract: A simplified method for the preparation of wheat germ agglutinin(WGA)-Sepharose 4B by coupling highly purified WGA, prepared by improved affinity chromatography, with BrCN activated Sepharose 4B in a solution of high carbonate buffer is described. The amount of WGA linked to Sepharose 4B was 82.40% (3.07 mg WGA per ml Sepharose 4B). MN blood group antigens of human erythrocyte membranes purified with WGA-Sepharose 4B affinity chromatography showed a single band on sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS/PAGE). The yield of the antigens from 400 mL fresh blood was 32-40 mg. The WGA-Sepharose 4B column could be used several times without loss of activity.
authors.affiliation: Department of Biochemistry, Second Military Medical College, Shanghai, People's Republic of China.
authors.forename: S M
authors.initials: SM
authors.lastname: Zhang
authors.forename: J X
authors.initials: JX
authors.lastname: Li
authors.forename: Y M
authors.initials: YM
authors.lastname: Wei
authors.forename: Z M
authors.initials: ZM
authors.lastname: Yang
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007519:Isoantigens / Q000302:isolation & purification*; D008951:MNSs Blood-Group System / Q000276:immunology*; D012685:Sepharose / Q000031:analogs & derivatives
chemical_list: D007519:Isoantigens; D008951:MNSs Blood-Group System; C042758:wheat germ agglutinin-sepharose 6MB; D012685:Sepharose