[The mutagenicity of organic microcontaminants in the environment. IV. The mutagenicity of volatile organic halogens in the SOS-chromotest].
To determine the sensitivity and responsibility of microbial shortterm-tests for detection of the mutagenic potency of volatile halogenated hydrocarbons (OHC) 18 pure substances from the groups of halomethanes, -ethanes and ethylenes were examined with the SOS-Chromotest. The strain Escherichia coli PQ37 was used in the standard procedure (macro procedure) with and without metabolic activation by Aroclor 1254 induced rat-liver microsomes. Although the selected OHC were tested to high doses, none of the tested compounds showed mutagenic effects in Escherichia coli PQ37. Also those OHC's, which showed positive results in the Ames test or being suspected of carcinogenicity, showed no positive results in the SOS-Chromotest. Therefore, the standard procedure of SOS-Chromotest is not suitable to detect the mutagenicity of volatile organic and halogenated compounds. Supplementary investigations with modified methods are necessary to check the results, because there is a good correlation between Ames test and SOS-Chromotest by testing other groups of compounds.
Mersch-Sundermann
V
V
Institut für Hygiene und Medizinische Mikrobiologie, Fakultät für Klinische Medizin Mannheim, Ruprecht-Karls-Universität Heidelberg.
Müller
G
G
Hofmeister
A
A
D000818:Animals; D004785:Environmental Pollutants / Q000633:toxicity*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics; D006846:Hydrocarbons, Halogenated / Q000633:toxicity*; D009152:Mutagenicity Tests; D009154:Mutation; D051381:Rats; D013014:SOS Response, Genetics; D014835:Volatilization
D004785:Environmental Pollutants; D006846:Hydrocarbons, Halogenated
Untersuchungen zur Mutagenität organischer Mikrokontaminationen in der Umwelt. IV. Mitteilung: Die Mutagenität leichtflüchtiger Organohalogene im SOS-Chromotest.