[Evaluation of the interleukin 1 production by adherent cells in patients with diabetes type II].
We have studied the comitogenic activity of IL 1 produced by cultures of mononuclear adherent cells obtained from Diabetes Mellitus (DM) type II or non insulin dependent diabetic patients. This activity was measured by the incorporation of 3H Thymidine into cultures of C3H/HeJ mice thymocytes in the presence of phytohemagglutinin (PHA). We have observed that IL 1 from patients with DM type II did not produce a synergistic effect with PHA, since the lymphoproliferation levels were similar to those obtained in the absence of this lectin. This lack of comitogenic activity (P less than 0.001) in relation to the response obtained with IL 1 from normal subjects plus PHA, could be due to the release of one or several soluble substances capable of blocking glycosylated receptors to mitogens or of impairing the cellular activation process.
Barañao
R I
RI
Tenenbaum
A
A
Tesone
P A
PA
Rumi
L S
LS
D000328:Adult; D000818:Animals; D003470:Culture Media; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D004926:Escherichia coli; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008809:Mice, Inbred C3H; D008875:Middle Aged; D010835:Phytohemagglutinins / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology
D003470:Culture Media; D007375:Interleukin-1; D008070:Lipopolysaccharides; D010835:Phytohemagglutinins; D013936:Thymidine
Evaluación de la producción de interluequina 1 por células adherentes en pacientes con diabetes tipo II.