[Enzymes of the phosphogluconate pathway in amebas].
Glucose-6-phosphate and 6-phosphogluconate dehydrogenases (G6PD and 6PGD) are revealed in Amoeba proteus by electrophoresis in polyacrylamide gels, thus proving the availability of the phosphogluconic pathway in amoebae. 6PGD is marked as a single band, and G6PD shows multiple banding. When an amoebic homogenate is obtained using Triton-100, a supplementary form of G6PD extracted from membranes of some cell organelles (presumably mitochondria) becomes apparent. Hexose-6-phosphate dehydrogenase seems to be absent and therefore all the G6PD forms found may be specific G6PDs proper.
Sopina
V A
VA
Podlipaeva
Iu I
IuI
D000656:Amoeba / Q000032:analysis / Q000201:enzymology*; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005942:Gluconates / Q000378:metabolism*; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D007527:Isoenzymes / Q000032:analysis; D009943:Organophosphorus Compounds / Q000378:metabolism*; D010734:Phosphogluconate Dehydrogenase / Q000032:analysis
D005942:Gluconates; D007527:Isoenzymes; D009943:Organophosphorus Compounds; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase
Fermenty fosfogliukonatnogo puti u ameb.