title: Binding of human IgA1 and IgA1 fragments to jacalin.
abstract: Interaction of jacalin, an N-terminal galactose specific lectin, with human IgA1 and IgA1 fragments was investigated. IgA1 and all galactose containing fragments bound to jacalin-Sepharose, including Fab fragments containing only the galNac linked to serine-224 and Fc fragments containing four gal-galNac sequences. These data indicate that both the galNac and gal-galNac sequences can interact with jacalin. Jacalin precipitated IgA1 and the fragments F(abc)2, F(ab')2 and Fc in agar gel and from solutions. It also precipitated Fab' fragments in agar gel. Jacalin did not precipitate Fab fragments significantly. This suggests that, except for the single binding site on the Fab fragments containing the galNac linked to serine-224, jacalin itself also has a limited number of sites to interact with N-terminal galactose residues. ELISA studies revealed that intact IgA1 had a lower jacalin binding capacity than F(abc)2 fragments which lack CH3 domains, than F(ab')2 which lack the CH2 and CH3 domains, and than Fc fragments containing four gal-galNac sequences. This led to the conclusion that part of the galNac or gal-galNac sequences in intact IgA1 molecules are inaccessible to interaction with jacalin. Cleaving the C-terminal domains off may have induced a reorientation of the hinge region structure, including the orientation of the carbohydrate units.
authors.affiliation: Department of Histology, Medical Faculty, Free University, Amsterdam, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: Biewenga
authors.forename: P S
authors.initials: PS
authors.lastname: Hiemstra
authors.forename: I
authors.initials: I
authors.lastname: Steneker
authors.forename: M R
authors.initials: MR
authors.lastname: Daha
mesh_terms: D000818:Animals; D001665:Binding Sites; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007070:Immunoglobulin A / Q000378:metabolism*; D007128:Immunoglobulin Fragments / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism*; D051379:Mice; D037121:Plant Lectins; D011233:Precipitin Tests
chemical_list: D007070:Immunoglobulin A; D007128:Immunoglobulin Fragments; D037102:Lectins; D037121:Plant Lectins; C043696:jacalin