Analysis of N-acetyl-4-O-acetylneuraminic-acid-containing N-linked carbohydrate chains released by peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase F. Application to the structure determination of the carbohydrate chains of equine fibrinogen.
The carbohydrate chains of equine fibrinogen were enzymatically released by peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase F. The oligosaccharides obtained were fractionated by a combination of FPLC and HPLC and analyzed by 500-MHz 1H-NMR spectroscopy. Four monosialo and four disialo diantennary N-acetyllactosamine type of carbohydrate chains occur: (formula; see text)
Damm
J B
JB
Department of Bio-Organic Chemistry, Utrecht University, The Netherlands.
Voshol
H
H
Hård
K
K
Kamerling
J P
JP
Vliegenthart
J F
JF
D000107:Acetylation; D000581:Amidohydrolases; D000818:Animals; D002236:Carbohydrate Conformation; D002241:Carbohydrates / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D005340:Fibrinogen / Q000032:analysis*; D006736:Horses; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009844:Oligosaccharides / Q000032:analysis*; D010446:Peptide Fragments / Q000032:analysis*; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D012794:Sialic Acids / Q000032:analysis*
D002241:Carbohydrates; D009844:Oligosaccharides; D010446:Peptide Fragments; D012794:Sialic Acids; C041901:4-O-acetyl-N-acetylneuraminic acid; D005340:Fibrinogen; D000581:Amidohydrolases; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase