Effect of electric field switching on the electrophoretic mobility of single-stranded DNA molecules in polyacrylamide gels.
We have examined the effects of pulsed electric fields on the separation of single-stranded DNA molecules in polyacrylamide sequencing gels. Using different electric field pulsing regimens, the mobilities of single-stranded DNA molecules can be retarded or increased as compared to conventional electrophoresis. These results indicated that pulsed field techniques can be applied to gel electrophoresis of small single-stranded DNA molecules.
Lai
E
E
Division of Biology, California Institute of Technology, Pasadena 91125.
Davi
N A
NA
Hood
L E
LE
D004277:DNA, Single-Stranded / Q000032:analysis*; D004574:Electromagnetic Fields; D055590:Electromagnetic Phenomena; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*
D004277:DNA, Single-Stranded