Determination of xylometazoline in plasma and urine by gas chromatography using a fused-silica capillary column and an electron-capture detector.
A sensitive method is described for the determination of unchanged xylometazoline in plasma and urine at concentrations down to 35 nmol/l. After addition of naphazoline as an internal standard, both compounds are extracted with dichloromethane-diethyl ether (20:80) at pH 10, back-extracted with an acidic solution and re-extracted from a sodium hydroxide solution with dichloromethane-diethyl ether (20:80). The compounds are then derivatized with heptafluorobutyric anhydride in the presence of pyridine. The derivatives are determined by capillary gas chromatography using electron-capture detection.
Sioufi
A
A
Laboratoires Ciba-Geigy, Biopharmaceutical Research Centre, Rueil-Malmaison, France.
Leroux
F
F
Dubois
J P
JP
D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D006801:Humans; D007093:Imidazoles / Q000097:blood / Q000378:metabolism* / Q000652:urine; D012015:Reference Standards
D007093:Imidazoles; C009695:xylometazoline