Derivatization and high-performance liquid chromatographic determination of urinary glycolic acid.
A high-performance liquid chromatographic method for the determination of urinary glycolic acid is proposed, based on pre-column derivatization with phenylhydrazine coupled with the enzymatic oxidation of glycolate to glyoxylate. The phenylhydrazone formed is separated by liquid chromatography and detected at 324 nm. The minimum detectable concentration of glycolate was 10.0 mumol/l. The recovery of glycolate added to urine averaged 96.1%. The day-to-day coefficients of variation calculated by analysis of two urine samples with normal and high glycolate contents were 4.6 and 7.5%, respectively. Results of analyses of urine samples from healthy persons, idiopathic calcium stone formers and Type I primary hyperoxaluria patients are reported.
Petrarulo
M
M
Laboratory of Renal Stone Disease, Ospedale Mauriziano Umberto I, Turin, Italy.
Pellegrino
S
S
Bianco
O
O
Marangella
M
M
Linari
F
F
Mentasti
E
E
D002851:Chromatography, High Pressure Liquid; D006016:Glycolates / Q000652:urine*; D006038:Glyoxylates / Q000378:metabolism; D006801:Humans; D006835:Hydrazones / Q000652:urine; D006959:Hyperoxaluria / Q000652:urine
D006016:Glycolates; D006038:Glyoxylates; D006835:Hydrazones; C012483:phenylhydrazone; C031149:glycolic acid; C031150:glyoxylic acid