title: Induction and repression of the Drosophila Sgs-3 glue gene are mediated by distinct sequences in the proximal promoter.
abstract: The normal developmental expression of the Drosophila salivary gland secretion protein gene Sgs-3 requires the interaction of a distal and proximal regulatory element. A deletion/replacement analysis of the proximal promoter in stably transformed lines shows that induction of an Sgs-3/Adh fusion gene is normal if sequences from +10 to -50 are replaced by those of the hsp70 gene. Sequences between -98 and -50 are necessary for this expression but there is internal redundancy within this region as two distinct upstream sequences of 18 and 22 bp respectively are sufficient for stage- and tissue-specific expression, albeit at reduced levels. A point mutation at -53 eliminates the ecdysone-mediated repression of the Sgs-3 promoter at pupariation. We report mosaicisms of expression within the salivary gland for a number of stably transformed lines.
authors.affiliation: Laboratoire de Génétique Moléculaire des Eucaryotes du C.N.R.S., Strasbourg, France.
authors.forename: M
authors.initials: M
authors.lastname: Martin
authors.forename: A
authors.initials: A
authors.lastname: Giangrande
authors.forename: C
authors.initials: C
authors.lastname: Ruiz
authors.forename: G
authors.initials: G
authors.lastname: Richards
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004330:Drosophila / Q000235:genetics*; D005786:Gene Expression Regulation; D005809:Genes, Regulator; D008969:Molecular Sequence Data; D009154:Mutation; D011401:Promoter Regions, Genetic
chemical_list: D004247:DNA