A spectrophotometric method for determining the stream sample core diameter of a flow cytometer.
A simple, rapid, and accurate method is described for the determination of flow cytometer stream sample core diameters. A precisely formulated solution of potassium dichromate is introduced as a sample, the stream is collected, and its absorbance is measured using a spectrophotometer. This permits the calculation of the factor by which the original solution was diluted and, given the stream diameter, the sample core diameter.
Blume
P
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Department of Pathology, University of New Mexico School of Medicine, Albuquerque 87131.
D005434:Flow Cytometry / Q000295:instrumentation*; D013053:Spectrophotometry / Q000379:methods*