title: Liquid chromatographic determination of ivermectin in bovine serum.
abstract: A rapid, sensitive method is described for the determination of ivermectin concentrations in bovine serum. Ivermectin is extracted by passing a sample through a reverse-phase C18 cartridge. A silica-packed cartridge is used to purify the extract further. Ivermectin is quantitated by liquid chromatography with detection at 245 nm. Recoveries were 95 +/- 4% for samples fortified with 20 ppb ivermectin. Concentrations as low as 2 ppb can be detected in 5 g samples.
authors.affiliation: U.S. Department of Agriculture, U.S. Livestock Insects Laboratory, Kerrville, TX 78029-0232.
authors.forename: D D
authors.initials: DD
authors.lastname: Oehler
authors.forename: J A
authors.initials: JA
authors.lastname: Miller
mesh_terms: D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid; D007202:Indicators and Reagents; D007559:Ivermectin / Q000097:blood*; D012997:Solvents
chemical_list: D007202:Indicators and Reagents; D012997:Solvents; D007559:Ivermectin