Introduction of a Micrococcus plasmid in Escherichia coli.
A 6-MDa plasmid (pMQV10), carrying cholesterol hydroxylase and streptomycin-resistance genes, from a gram-positive strain of Micrococcus sps., (RJ6) has been successfully transformed in gram-negative Escherichia coli K12 C600. pMQV10 is maintained stably and expresses its drug resistance in the new host.
Verma
V
V
Genetic Engineering Unit, Regional Research Laboratory, Jammu-Tawi, India.
Qazi
G N
GN
Parshad
R
R
Chopra
C L
CL
D002790:Cholesterol 7-alpha-Hydroxylase / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D008837:Micrococcus / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D010957:Plasmids; D013307:Streptomycin / Q000494:pharmacology; D014169:Transformation, Bacterial
D002790:Cholesterol 7-alpha-Hydroxylase; D013307:Streptomycin