Acetylaminofluorene-labelled ribosomal RNA for use in molecular epidemiology and taxonomy.
The use of acetylaminofluorene-labelled 16 + 23S rRNA (from Escherichia coli) is described for determining rRNA-gene-restriction patterns. The labelled probe allowed molecular fingerprinting of bacteria belonging to diverse phylogenetic branches (Enterobacteriaceae, Haemophilus, Pseudomonas, Acinetobacter, Brucella, Leptospira, Cytophaga, Campylobacter, Methylophaga). The labelled probe can be stored frozen (-20 degrees C) for at least a year and can endure vacuum dessication, ethanol precipitation or lyophilization.
Grimont
F
F
Unité 199 INSERM, Institut Pasteur, Paris.
Chevrier
D
D
Grimont
P A
PA
Lefevre
M
M
Guesdon
J L
JL
D015073:2-Acetylaminofluorene; D001419:Bacteria / Q000145:classification* / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli; D009710:Nucleotide Mapping; D015347:RNA Probes; D012335:RNA, Ribosomal; D015183:Restriction Mapping
D015347:RNA Probes; D012335:RNA, Ribosomal; D015073:2-Acetylaminofluorene