Relationship between hepatic glutathione content and carbon tetrachloride-induced hepatotoxicity in vivo.
The relationship between carbon tetrachloride (CCl4)-induced hepatotoxicity and hepatic glutathione (GSH) content was investigated in fed and fasted rats. The elevation of serum glutamic-pyruvic transaminase (GTP) activity by CCl4 treatment was enhanced by fasting. Although the hepatic GSH content fo 12-hour-fasted rats was higher than that of fed rats determined at 6 p.m., the serum GPT activity of the former was higher than that of the latter. Starvation had no effect on the activities of hepatic glutathione peroxidase (GSH-Px) and glutathione reductase (GR). The results suggest that the potentiation of hepatic injury by CCl4 cannot be related to hepatic GSH content.
Shimuzu
M
M
Osaka City Institute of Public Health and Environmental Sciences, Japan.
Morita
S
S
Yamano
T
T
Yamada
A
A
D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D002251:Carbon Tetrachloride / Q000633:toxicity*; D056486:Chemical and Drug Induced Liver Injury; D005215:Fasting; D005978:Glutathione / Q000032:analysis*; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D008099:Liver / Q000032:analysis* / Q000201:enzymology; D008107:Liver Diseases / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D002251:Carbon Tetrachloride; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D000410:Alanine Transaminase; D005978:Glutathione