Radiation-induced volatile hydrocarbon production in platelets.
Generation of volatile hydrocarbons (ethane, pentane) as a measure of lipid peroxidation was followed in preparations from platelet-rich plasma irradiated in vitro. The hydrocarbons in the headspace of sealed vials containing irradiated and nonirradiated washed platelets, platelet-rich plasma, or platelet-poor plasma increased with time. The major hydrocarbon, pentane, increased linearly and significantly with increasing log radiation dose, suggesting that reactive oxygen species induced by ionizing radiation result in lipid peroxidation. Measurements of lipid peroxidation products may give an indication of suboptimal quality of stored and/or irradiated platelets.
Radha
E
E
Radiation Biochemistry Department, Armed Forces Radiobiology Research Institute, Bethesda, MD 20814-5145.
Vaishnav
Y N
YN
Kumar
K S
KS
Weiss
J F
JF
D001792:Blood Platelets / Q000378:metabolism / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D004980:Ethane / Q000097:blood*; D006801:Humans; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000528:radiation effects*; D010420:Pentanes / Q000097:blood*; D012016:Reference Values
D010420:Pentanes; D004980:Ethane