Repetitive DNA probes for the detection of Babesia equi.
This report describes DNA probes for the identification of Babesia equi. A genomic library of B. equi was constructed in pUC13. Several clones were identified that hybridized strongly to B. equi DNA. Clone pBE33 hybridized specifically to B. equi DNA and did not hybridize to horse DNA nor to DNA from Babesia caballi, Babesia bovis or Babesia bigemina. Two subclones of pBE33 (pSB20 and pEH21) containing B. equi repetitive sequences, could detect 0.49 ng and 0.97 ng B. equi DNA, respectively.
Posnett
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Molecular Biology Section, Veterinary Research Institute, Onderstepoort, South Africa.
Ambrosio
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D000818:Animals; D001403:Babesia / Q000235:genetics / Q000302:isolation & purification*; D015139:Blotting, Southern; D004247:DNA / Q000032:analysis* / Q000235:genetics; D015342:DNA Probes; D004587:Electrophoresis, Agar Gel; D006736:Horses; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping
D015342:DNA Probes; D004247:DNA