[Monoclonal antibodies to the muscle isoform of alpha-actinin--a marker for the study of the differentiation of skeletal and cardiac muscles].
A battery of monoclonals to the rabbit skeletal muscle alpha-actinin has been produced. The majority of monoclonals proved to be species-specific by indirect immunofluorescence on the isolated rabbit skeletal myofibrils and on the differentiating cultures of chicken and rat skeletal muscles. One monoclonal, EA-53, reacts with the skeletal muscle alpha-actinin of various species (rat, rabbit, chicken) in immunofluorescence and immunoblotting. The monoclonal EA-53 recognizes also heart muscle alpha-actinin in cultured cardiomyocytes of human, rat and mouse origin. EA-53 does not stain alpha-actinin in myoblasts, fibroblasts, and endothelial cells. The monoclonal antibody EA-53 discriminating muscle and nonmuscle alpha-actinin isoforms could be used as a tool to study the mechanisms of skeletal and cardiac myogenesis.
Fridlianskaia
I I
II
Goncharova
E I
EI
Borisov
A B
AB
Krylova
T A
TA
Pinaev
G P
GP
D000185:Actinin / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000302:isolation & purification*; D015415:Biomarkers / Q000032:analysis; D002454:Cell Differentiation / Q000276:immunology; D002478:Cells, Cultured / Q000276:immunology; D002645:Chickens; D005455:Fluorescent Antibody Technique; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D015151:Immunoblotting; D051379:Mice; D008807:Mice, Inbred BALB C; D009132:Muscles / Q000166:cytology / Q000276:immunology*; D009206:Myocardium / Q000166:cytology / Q000276:immunology*; D011817:Rabbits; D051381:Rats; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D000185:Actinin
Monoklonal'nye antitela k myshechnoĭ izoforme al'fa-aktinina--marker dlia izucheniia differentsirovki skeletnoĭ i serdechnoĭ myshts.