Nucleosome reconstitution of core-length poly(dG).poly(dC) and poly(rG-dC).poly(rG-dC).
The double-stranded polypurine.polypyrimidines poly(dG).poly(dC) and poly[d(A-G)].poly[d(T-C)] and the mixed ribose-deoxyribose polynucleotide poly(rG-dC).poly(rG-dC) have been successfully reconstituted into nucleosomes. The radioactively labeled particles comigrate in gel electrophoresis and sucrose density gradient experiments with authentic nucleosomes derived from chicken erythrocyte chromatin. These results show that nucleosomes are able to accommodate a wider variety of polynucleotides than was previously believed.
Jayasena
S D
SD
Department of Chemistry, Lehigh University, Bethlehem, Pennsylvania 18015.
Behe
M J
MJ
D000818:Animals; D001482:Base Composition; D002645:Chickens; D002843:Chromatin / Q000648:ultrastructure; D003850:Deoxyribonuclease I; D004912:Erythrocytes; D009707:Nucleosomes / Q000648:ultrastructure*; D011089:Polydeoxyribonucleotides; D011119:Polynucleotides
D002843:Chromatin; D009707:Nucleosomes; D011089:Polydeoxyribonucleotides; D011119:Polynucleotides; C016681:poly(dG).poly(dC); C043827:poly(rG-dC).poly(rG-dC); D003850:Deoxyribonuclease I