Solid-phase synthesis of oligoribonucleotides using 9-fluorenylmethoxycarbonyl (Fmoc) for 5'-hydroxyl protection.
Efficient solid-phase synthesis of a series of oligoribonucleotides of up to 20 residues is described that utilises the 9-fluorenylmethoxycarbonyl group (Fmoc) for 5'-protection and 4-methoxytetrahydropyran-4-yl (Mthp) for 2'-protection of ribonucleotide monomers and a phosphoramidite coupling procedure. The Fmoc group is removed after each coupling step by treatment with 0.1M DBU in acetonitrile. Oligoribonucleotides are isolated in 2'-protected form in good yield and shown to be readily and efficiently deprotected by mild acidic treatment.
Lehmann
C
C
Medical Research Council, Laboratory of Molecular Biology, Cambridge, UK.
Xu
Y Z
YZ
Christodoulou
C
C
Tan
Z K
ZK
Gait
M J
MJ
D001483:Base Sequence; D003432:Cross-Linking Reagents; D005449:Fluorenes; D008969:Molecular Sequence Data; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D010755:Organophosphates; D009943:Organophosphorus Compounds / Q000138:chemical synthesis; D012263:Ribonucleosides / Q000138:chemical synthesis; D013387:Succinic Anhydrides
D003432:Cross-Linking Reagents; D005449:Fluorenes; D009843:Oligoribonucleotides; D010755:Organophosphates; D009943:Organophosphorus Compounds; D012263:Ribonucleosides; D013387:Succinic Anhydrides