Suppression of macrophage lysosomal enzymes after Leishmania donovani infection.
In order to have an insight into the role of host lysosomal enzymes in the intracellular survival of Leishmania parasites, the activities of beta-galactosidase, alpha-mannosidase, and N-acetyl-beta-D-glucosaminidase were studied in peritoneal macrophages of hamsters infected with L. donovani. There was a significant decrease of all three lysosomal enzymes after infection. Heat-killed or formalin-treated parasites failed to inhibit the enzymes, instead a slight stimulation was observed. Purified excreted factor from promastigotes had no effect on the enzymes except beta-galactosidase which was inhibited up to 20%. Inhibition of enzymes was not due to increased secretion after infection. The absence of induction of any endogenous macrophage inhibitor was confirmed by mixed experiments. The levels of 5'-nucleotidase and lactate dehydrogenase remained unchanged after infection. Thus, the inhibition of lysosomal enzymes appears to be the effect of infection process and reflects to actua decrease rather than increased secretion or the action of any inhibitors present in Leishmania promastigotes.
Chakraborty
P
P
Leishmania Group, Indian Institute of Chemical Biology, Calcutta.
Das
P K
PK
D004798:Enzymes / Q000378:metabolism*; D006867:Hydrolases / Q000378:metabolism; D007898:Leishmaniasis, Visceral / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology*; D008264:Macrophages / Q000201:enzymology* / Q000469:parasitology
D004798:Enzymes; D006867:Hydrolases