Epithelial modulation of non-adrenergic, non-cholinergic and vasoactive intestinal peptide-induced responses: role of neutral endopeptidase.
Relaxation of guinea-pig trachea was induced by vasoactive intestinal peptide (VIP) or electrical field stimulation. Mechanical removal of airway epithelium potentiated responses to VIP and attenuated inhibitory non-adrenergic, non-cholinergic (i-NANC) responses to low stimulation frequencies. Phosphoramidon potentiated responses to VIP in intact but not de-epithelialised preparations, and had no effect on i-NANC responses. These findings suggest that neutral endopeptidase localised to the epithelium may modulate relaxation of guinea-pig trachea induced by VIP but not i-NANC nerve-stimulation.
Rhoden
K J
KJ
National Heart and Lung Institute, London, U.K.
Barnes
P J
PJ
D000818:Animals; D001341:Autonomic Nervous System / Q000502:physiology*; D004558:Electric Stimulation; D010450:Endopeptidases / Q000502:physiology*; D004848:Epithelium / Q000502:physiology; D005289:Ferrets; D006020:Glycopeptides / Q000494:pharmacology; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D009126:Muscle Relaxation / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology; D013779:Tetrodotoxin / Q000494:pharmacology; D014132:Trachea / Q000187:drug effects / Q000502:physiology; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology*
D006020:Glycopeptides; D014660:Vasoactive Intestinal Peptide; D013779:Tetrodotoxin; D010450:Endopeptidases; C008890:phosphoramidon