title: Interaction between cortisol and microbial proteases.
abstract: Binding (or interaction) of cortisol with microbial molecule(s) was observed by employing Bio-Gel HTP affinity chromatography and subsequently by fluorescence spectrophotometry. Molecule(s) in the crude extract of baker's yeast and in other microbial proteases exhibited varied degrees of cortisol-binding. Bacterial protease (type IX) had highest, while the type XXVI enzyme had the lowest, binding capacity. In addition, these two proteases exhibited a distinct difference in the alterations of ultraviolet spectra due to interaction with cortisol. Using casein as a substrate, cortisol, CTP, trypsin inhibitor or leupeptin appreciably inhibited type IX protease at low concentrations of Ca2+. However, thyroxine had no effect on this protease.
authors.affiliation: Division of Science and Mathematics, Bethune-Cookman College, Daytona Beach, Florida.
authors.forename: W N
authors.initials: WN
authors.lastname: Kuo
authors.forename: U
authors.initials: U
authors.lastname: Ganesan
authors.forename: M N
authors.initials: MN
authors.lastname: Jean
authors.forename: T B
authors.initials: TB
authors.lastname: Richardson
authors.forename: A
authors.initials: A
authors.lastname: Robinson
authors.forename: A
authors.initials: A
authors.lastname: Williams
authors.forename: N
authors.initials: N
authors.lastname: Stone
authors.forename: M
authors.initials: M
authors.lastname: Young
authors.forename: J
authors.initials: J
authors.lastname: Noone
authors.forename: E J
authors.initials: EJ
authors.lastname: Burch
mesh_terms: D001419:Bacteria / Q000201:enzymology*; D010450:Endopeptidases / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005658:Fungi / Q000201:enzymology*; D006854:Hydrocortisone / Q000378:metabolism*; D066298:In Vitro Techniques
chemical_list: D004791:Enzyme Inhibitors; D010450:Endopeptidases; D006854:Hydrocortisone