Changes in glutathione status and 3,5,3'-triiodothyronine action in livers of rats given cysteine-deficient diets.
1. For a period of 32 d young rats were given a diet containing (g/kg) 220 casein, 120 casein + 1.93 L-cysteine (Cys), or 120 casein. 2. The formation of 3,5,3'-triiodothyronine (T3)-nuclear protein complexes was reduced in rats fed on the Cys-deficient diet. 3. Scatchard analysis showed that decreased formation of T3-nuclear protein complexes was due to a decreased affinity of T3 receptors; this decrease was induced, at least in part, by a reduced glutathione content. 4. In rats fed on the Cys-deficient diet there was an expected decrease in growth but an unexpected increase in the activities of glucose-6-phosphate dehydrogenase (EC 1.1.1.49) and malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) (EC 1.1.1.40). It is suggested that this increase is related to an increased oxidized glutathione:reduced glutathione ratio.
Garcin
H
H
Département Alimentation et Nutrition, Université de Bordeaux I, Talence, France.
Suberville
C
C
Higueret
P
P
Higueret
D
D
D000818:Animals; D003545:Cysteine / Q000008:administration & dosage*; D004032:Diet; D005978:Glutathione / Q000378:metabolism*; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D009687:Nuclear Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011988:Receptors, Thyroid Hormone / Q000378:metabolism; D014284:Triiodothyronine / Q000378:metabolism*
D009687:Nuclear Proteins; D011988:Receptors, Thyroid Hormone; D014284:Triiodothyronine; D005978:Glutathione; D003545:Cysteine