A new method for preparation of undecalcified bone sections.
A new method for preparation of sections of undecalcified bone is described. Samples of ovine bone were embedded in methylmethacrylate and thick-sectioned with a cutoff machine or commercial band saw. Composite slides were prepared by gluing white acrylic to glass using cyanoacrylate glue. Bone sections were glued to the composite slide and then surface polished by grinding or ultramilling. The polished surface of the section was then etched and stained. The techniques described in this paper reduce the time spent grinding or milling sections and improve resolution of surface-stained features of undecalcified bone sections.
Sterchi
D L
DL
Department of Veterinary Biosciences, University of Illinois, Urbana 61801.
Eurell
J A
JA
D000818:Animals; D001842:Bone and Bones / Q000166:cytology*; D006652:Histological Techniques; D020366:Methylmethacrylate; D008768:Methylmethacrylates; D008867:Microtomy / Q000379:methods*; D012756:Sheep
D008768:Methylmethacrylates; D020366:Methylmethacrylate