title: Gas chromatographic determination of gallopamil and norgallopamil in human plasma.
abstract: A highly sensitive gas chromatographic assay is described for the simultaneous determination of gallopamil, a calcium channel blocking agent, and its major metabolite, norgallopamil. A multi-step extraction procedure is employed followed by on-column capillary gas chromatographic analysis using nitrogen-selective detection. Acetylation of norgallopamil is performed to enable accurate quantification of the metabolite. Linearity was achieved over the range 1-50 ng/ml for both analytes. Assay specificity, precision and accuracy were investigated.
authors.affiliation: Department of Biopharmaceutics, Schering Research, Pembroke Pines, FL 33023.
authors.forename: D A
authors.initials: DA
authors.lastname: Ruggirello
authors.forename: P K
authors.initials: PK
authors.lastname: Noonan
authors.forename: M A
authors.initials: MA
authors.lastname: Gonzalez
authors.forename: P
authors.initials: P
authors.lastname: Ho
authors.forename: J G
authors.initials: JG
authors.lastname: Wagner
mesh_terms: D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D005711:Gallopamil / Q000031:analogs & derivatives* / Q000097:blood*; D006801:Humans
chemical_list: C052471:norgallopamil; D005711:Gallopamil