Transgenic approach for the study of pathogenesis induced by human viruses.
An understanding of the pathogenesis of human viral diseases has been hampered by the lack of suitable animal models. However, with the advent in the last decade of transgenic technology, it is now possible to introduce one or more viral genes into the germ-line of animals. Thus, transgenic technology allows for the study of viral gene expression and function in the context of the whole animal. The focus of this review is to define the advantages and disadvantages of the transgenic approach in studies of viral pathogenesis. Studies involving a human DNA tumor virus (JCV) and a human retrovirus (HIV) will be described to illustrate these points.
Lassam
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N
Laboratory of Virology, Jerome H. Holland Laboratory, American Red Cross, Rockville, MD 20855.
Feigenbaum
L
L
Vogel
J
J
Jay
G
G
D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D006801:Humans; D012190:Retroviridae / Q000235:genetics / Q000472:pathogenicity; D014777:Virus Diseases / Q000235:genetics*