Techniques of sperm selection, improvement and separation in an in vitro fertilization program.
The Authors present a review of the various techniques used for improving the fertility potential of sperm within the context of an in vitro fertilization (IVF) program. After a brief description of the mechanisms leading to normal in vivo fertilization, they discuss the different methods of selecting and improving sperm for IVF. They conclude that centrifugation on discontinuous Percoll gradients would seem to be the most efficient separation method from all points of view, while the addition of pharmacological agents to improve sperm quality and motility lead to extremely unsatisfactory results.
Van der Zwalmen
P
P
Openbaar Centrum voor Maatschappelijk Welzijn Genees- en Heelkundig Instituut, Vilvoorde.
Bertin
G
G
Schoysman-Deboeck
A
A
Geerts
L
L
Schoysman
R
R
Debauche
C
C
D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D003470:Culture Media; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D013048:Specimen Handling; D013075:Sperm Capacitation; D013081:Sperm Motility; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000002:abnormalities / Q000187:drug effects
D003470:Culture Media