A galactose-specific lectin from the hemolymph of the pearl oyster, Pinctada fucata martensii.
1. A lectin in the serum of Pinctada fucata martensii was purified by a combination of affinity chromatography on Sepharose 4B coupled with bovine submaxillary gland mucine, anion exchange chromatography on Mono Q and gel filtration on Superose 6. 2. The purified lectin was indicated to be homogeneous by polyacrylamide electrophoresis and rechromatography on Mono Q. 3. The purified lectin was approximately 440,000 in molecular weight and was composed of identical subunits with a molecular weight of approximately 20,000. 4. D-galactose and N-acetylgalactosamine gave a 50% inhibition of agglutination of horse erythrocytes by the lectin at 0.3 and 1.2 mM, respectively. 5. The antibody obtained from rabbit immunized with the purified lectin was monospecific to the lectin judged from the hemagglutination blocking test, immunoelectrophoresis and immunoblotting.
Suzuki
T
T
National Research Institute of Aquaculture, Mie, Japan.
Mori
K
K
D000818:Animals; D002118:Calcium; D003429:Cross Reactions; D037161:Galectins; D006388:Hemagglutinins / Q000276:immunology / Q000302:isolation & purification*; D006458:Hemolymph / Q000276:immunology; D007120:Immunochemistry; D015394:Molecular Structure; D008970:Molecular Weight; D010125:Ostreidae / Q000276:immunology*; D013696:Temperature
D037161:Galectins; D006388:Hemagglutinins; D002118:Calcium