Retention of immunogenicity after X-irradiation of mouse colon tumor cells expressing the transfected influenza virus hemagglutinin gene.
We have previously demonstrated that murine colon tumor cells transfected with the gene coding for the hemagglutination antigen (HA) of influenza virus acquire an inherent immunogenicity, fail to grow in syngeneic mice, and demonstrate an ability to cross-protect against a challenge with parental nontransfected cells. In the present study the immunogenic potential of HA-transfected cells correlated with cell-surface HA expression, as measured both by a fluorescence-activated cell sorter and by radio-labeled antibody binding. HA-transfected immunogenic cells had a median lethal dose (LD50) that was 10,000-fold greater than that of nontransfected cells. Most importantly this study demonstrated that HA-transfected cells retained their immunogenicity after X-irradiation with 12,000 rad. This characteristic makes their potential usefulness in treating human neoplasia more plausible.
Itaya
T
T
Department of Cell Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030.
Hunt
B
B
Frost
P
P
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology / Q000528:radiation effects*; D001666:Binding Sites, Antibody; D002460:Cell Line; D002999:Clone Cells / Q000276:immunology / Q000528:radiation effects / Q000637:transplantation; D015179:Colorectal Neoplasms / Q000276:immunology*; D005260:Female; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral / Q000235:genetics / Q000528:radiation effects*; D009980:Influenza A virus / Q000276:immunology / Q000528:radiation effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D014162:Transfection / Q000528:radiation effects*
D000956:Antigens, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral