Tacaribe virus L gene encodes a protein of 2210 amino acid residues.
The nucleotide sequence of Tacaribe virus (TV) L gene was obtained from two sets of overlapping cDNA clones constructed by walking along the virus L RNA using two successive synthetic DNA primers. Analysis of the sequence indicated the existence of a unique long open reading frame in the viral complementary strand. The first in-phase AUG codon is in positions 31-33 from the 5' end of the viral complementary L RNA surrounded by a sequence favorable for initiation of protein synthesis. The open reading frame ends at positions 6661-6663. The predicted TV L protein is a 2210 amino acid long polypeptide with an estimated molecular weight of 251,942. Comparison of the amino acid sequence of TV L protein with peptide sequences predicted from L-derived cDNA clones of lymphocytic choriomeningitis virus shows an overall 42% of homology.
Iapalucci
S
S
Centro de Virologia Animal, Buenos Aires, Argentina.
Lopez
R
R
Rey
O
O
Lopez
N
N
Franze-Fernandez
M T
MT
Cohen
G N
GN
Lucero
M
M
Ochoa
A
A
Zakin
M M
MM
D000595:Amino Acid Sequence; D001116:Arenaviridae / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D008217:Lymphocytic choriomeningitis virus / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009975:Orthomyxoviridae / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics*
D004279:DNA, Viral; D014764:Viral Proteins