Differential localization of microtubules in cerebellar cells.
The distribution and subcellular localization of microtubules in rat brain cerebellum was analyzed by immunohistochemistry with antibodies prepared against 3 synthetic peptides corresponding to the C-terminal region of beta-tubulin. The peptides used correspond to amino acid positions 416-425 (peptide 1), 416-431 (peptide 2), and 426-445 (peptide 4). The antibodies thus obtained displayed a remarkable specificity in reacting with different cell types in the rat cerebellum. Antibodies directed against peptide 1 primarily stained Purkinje cells and their dendrites and axons. Peptide 2 antibodies preferentially stained the glomeruli, while antibodies directed against peptide 4 preferentially stained Bergmann glial fibers. These results are discussed in terms of dissimilarities in microtubule organization and masking of epitopes by microtubule-associated proteins (MAPs) in individual cerebellar cells, which may be related to specific functional properties.
Ginzburg
I
I
Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel.
Behar
L
L
Littauer
U Z
UZ
Griffin
W S
WS
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology; D002531:Cerebellum / Q000166:cytology* / Q000648:ultrastructure; D015151:Immunoblotting; D007150:Immunohistochemistry; D008870:Microtubules / Q000648:ultrastructure*; D009474:Neurons / Q000648:ultrastructure*; D009479:Neuropeptides / Q000235:genetics / Q000276:immunology; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000235:genetics / Q000276:immunology; D014018:Tissue Distribution; D014404:Tubulin / Q000235:genetics
D000906:Antibodies; D009479:Neuropeptides; D010446:Peptide Fragments; D014404:Tubulin