The molecular cloning of the complementary deoxyribonucleic acid for bovine vitamin D-dependent calcium-binding protein: structure of the full-length protein and evidence for homologies with other calcium-binding proteins of the troponin-C superfamily of proteins.
We have cloned the cDNA for bovine intestinal vitamin D-dependent calcium-binding protein and, based on the sequence of the DNA, have deduced the structure of the full-length protein. The sequence of the cDNA clone predicts a protein comprised of 78 amino acids with a mol wt of 8788. The mRNA for the protein in bovine duodenum is about 500-600 bases in length. The protein sequence of bovine intestinal calcium-binding protein is 87% homologous with the sequence of porcine intestinal vitamin D-dependent calcium-binding protein and 81% homologous with the sequence of rat intestinal vitamin D-dependent calcium-binding protein. Hydrophilicity plots of the proteins noted above show that despite differences in amino acid sequence the proteins have similar patterns. In addition, the predicted secondary structure of the proteins is similar. Bovine intestinal calcium-binding protein shows 48.6% homology with the alpha-chain and 38.2% homology with the beta-chain of bovine S-100 protein and a similar high degree of homology with the beta-chain of human S-100 protein. The protein also demonstrates 36-43% homology with parvalbumin alpha and beta from various species and with troponin-C. There is some homology with the 28K vitamin D-dependent calcium-binding proteins. Vitamin D-dependent bovine intestinal calcium-binding protein is closely related to other mammalian intestinal calcium-binding proteins and to the S-100 proteins, parvalbumins, and troponin-C.
Kumar
R
R
Department of Medicine, Mayo Clinic, Rochester, Minnesota 55905.
Wieben
E
E
Beecher
S J
SJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000032:analysis / Q000235:genetics*; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D007413:Intestinal Mucosa / Q000032:analysis / Q000166:cytology; D051379:Mice; D008969:Molecular Sequence Data; D012317:RNA Phages; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D064030:S100 Calcium Binding Protein G / Q000032:analysis / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D014336:Troponin / Q000032:analysis / Q000235:genetics*; D019209:Troponin C
D002135:Calcium-Binding Proteins; D012333:RNA, Messenger; D064030:S100 Calcium Binding Protein G; D014336:Troponin; D019209:Troponin C; D004247:DNA