title: [Identification and localization of exopeptidase activities bound to membranes of Acinetobacter calcoaceticus].
abstract: After gel filtration the supernatant of Triton X-100 treated membrane sediments of Acinetobacter calcoaceticus shows activities for alanyl aminopeptidase, leucyl aminopeptidase, glutamyl aminopeptidase, prolyl aminopeptidase, aminopeptidase My, gamma-glutamyl arylamidase, dipeptidylpeptidase IV and prolyl carboxypeptidase. The intracellular localization of the enzymes was analyzed by sucrose density gradient centrifugation of the DNase/RNase pretreated membrane sediments. Most of the exopeptidases are located in the inner membrane fractions (band L). Only gamma-glutamyl arylamidase is located with its main activity in the cytoplasmic membrane (band M). Only small exopeptidase activities could be found in the outer membrane (band H).
authors.affiliation: Institut für Biochemie, Bereich Medizin, Martin-Luther-Universität Halle-Wittenberg, Saale.
authors.forename: G
authors.initials: G
authors.lastname: Jahreis
authors.forename: H
authors.initials: H
authors.lastname: Aurich
mesh_terms: D000150:Acinetobacter / Q000201:enzymology*; D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D020689:Exopeptidases; D008565:Membrane Proteins / Q000378:metabolism; D010447:Peptide Hydrolases / Q000032:analysis*; D012995:Solubility
chemical_list: D008565:Membrane Proteins; D020689:Exopeptidases; D010447:Peptide Hydrolases
vernacular_title: Nachweis und Lokalisation von Exopeptidase-Aktivitäten in Membranen von Acinetobacter calcoaceticus.