[Changes in the neurite-glial interrelations under the influence of antiserum against galactocerebrosides (a vital tissue culture study)].
Two--five-day-old culture of 10-11-day-old chick embryos has been used. Antiserum against galactocerebrosides (anti-GalC) is added to the nutrition medium before cultuvation. In the presence of anti-GalC the growth and gliocyte migration zone decreases, the area index becomes essentially small (3.5 +/- 0.7 in the control, 1.5 +/- 0.5 in the experiment). This is connected with inhibition of migration and proliferation of gliocytes during first 48 h of cultivation, while intensity of neuron regeneration remains unchanged. Alterations of the neurit-glial relations are investigated by means of the vital phase-contrast microscopy. Effect of anti-GalC to peripheral gliocytes is accompanied with a decreasing adhesive ability of their plasmolemma. This makes difficult their flattening on the neurit membrane, formation of contact membranous neurit-glial relations and formation of glial membranes. On the 3d day formation of nervous fasciculi is retarded, as well as their fusion into trunks and plexuses. On the 5th day, unlike the control, a continuous "epineural" covering of neuritic plexuses does not form. Round retractile and defective (with protrusions) forms of gliocytes predominate. These data demonstrate inhibitory effect of anti-GalC on the structural-functional maturation of the glia and on formation of neurit-glial relations in the culture of the sensitive ganglion.
Sotnikov
O S
OS
Taranova
N P
NP
Chalisova
N I
NI
Luchakova
D000818:Animals; D002642:Chick Embryo; D005696:Galactosidases / Q000276:immunology*; D005698:Galactosylceramidase / Q000276:immunology*; D007106:Immune Sera; D009441:Neurilemma / Q000166:cytology / Q000276:immunology*; D009474:Neurons / Q000166:cytology / Q000276:immunology*
D007106:Immune Sera; D005696:Galactosidases; D005698:Galactosylceramidase
Izmeneniia neĭrit-glial'nykh vzaimootnosheniĭ pri vozdeĭstvii antisyvorotki k galaktotserebrozidam (prizhiznennoe issledovanie kul'tury tkani).