title: Postlabeling analysis of indigenous aromatic DNA adducts in mouse myocardium during aging.
abstract: The possible presence of aromatic chemicals covalently linked to DNA (aromatic adducts) was investigated in heart cells during aging of the C57BL/6Nia mouse. Heart DNAs were isolated from untreated mice of different ages and analyzed by 32P-postlabeling assays. To determine low levels of adducts, assays were carried out in which aromatic adducts were first isolated by phase transfer to 1-butanol, then labeled with excess, carrier-free [gamma-32P]ATP. This analysis showed that the number and frequency of aromatic adducts varied between DNA samples. Several adducts were present in all mouse DNA preparations and were more abundant in 32P-maps of senescent heart DNA. The results suggest that genomes of myocytes have a higher steady-state level of DNA damage in old animals which could adversely affect cell function.
authors.affiliation: Department of Biochemistry, University of South Alabama, College of Medicine, Mobile 36688.
authors.forename: J W
authors.initials: JW
authors.lastname: Gaubatz
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D004247:DNA / Q000032:analysis*; D004249:DNA Damage; D004260:DNA Repair; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009206:Myocardium / Q000032:analysis*; D010761:Phosphorus Radioisotopes
chemical_list: D010761:Phosphorus Radioisotopes; D004247:DNA