Antiproliferative mechanism of anti-class II monoclonal antibodies.
The function of Class II molecules in proliferation was explored by treating human cell lines with three distinct anti-DR monoclonal antibodies (MABs). Dose-dependent, specific inhibition of eight DR+ cell lines of different origin and lineage was found. Inhibition was durable (i.e., cells did not become resistant to the anti-DR MABs despite prolonged treatment) yet reversible. The mechanism of inhibition was not due to differentiation or killing but was cytostatic. Inhibition was temporally associated with decreases in nuclear size and irregularity and appeared to be due to a non-phase-specific cell cycle arrest.
Vaickus
L
L
Department of Medicine, University of Iowa College of Medicine, Iowa City.
Jones
V E
VE
Morton
C L
CL
Whitford
K
K
Bacon
R N
RN
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002453:Cell Cycle; D002455:Cell Division; D002460:Cell Line; D002467:Cell Nucleus / Q000473:pathology; D006684:HLA-DR Antigens / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D006684:HLA-DR Antigens