title: Expression of post-transcriptional regulatory gene of HTLV-I, rex, in Escherichia coli.
abstract: Human T-cell leukemia virus type-I (HTLV-I) has a post-transcriptional regulatory gene termed rex. We have designed the rex gene to express in E. coli. Synthesis of rex protein, p27rex, was examined by immunoblot analysis using anti-p27rex antibody. No difference in electrophoretic mobility in NaDadSO4-PAGE was observed between p27rex expressed in E. coli and in an HTLV-I-infected cell line, MT-2. Slower migration of p27rex, corresponding to a 27-kD protein, in NaDodSO4-PAGE when compared with the calculated molecular weight from the amino acid sequence (Mr = 20,367) is suggested to be caused not by post-translational modification, but by the intrinsic nature of the protein, which is rich in proline and arginine.
authors.affiliation: Institute for Virus Research, Kyoto University, Japan.
authors.forename: Z A
authors.initials: ZA
authors.lastname: Zhou
authors.forename: Y
authors.initials: Y
authors.lastname: Adachi
authors.forename: M
authors.initials: M
authors.lastname: Maki
authors.forename: M
authors.initials: M
authors.lastname: Hatanaka
mesh_terms: D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005809:Genes, Regulator; D015368:Human T-lymphotropic virus 1 / Q000235:genetics*; D015151:Immunoblotting; D012323:RNA Processing, Post-Transcriptional; D012367:RNA, Viral / Q000378:metabolism; D015534:Trans-Activators / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D012367:RNA, Viral; D015534:Trans-Activators