Leukocyte accumulation in sparganosis: further characterization of an eosinophil chemotactic factor of the plerocercoid of Spirometra erinacei.
An eosinophil chemotactic (ECF) was partially purified from plerocercoids of Spirometra erinacei by a combination of anion-exchange chromatography on DE52 and gel filtration on Sephacryl S-200. The molecular weight of ECF was estimated to be 25,000-45,000 by high-pressure liquid chromatography. The ECF was bound with concanavalin A-Sepharose. The ECF was sensitive to periodate oxidation and to heating (56 degrees C, 30 min). On isoelectric focusing, eosinophil chemotactic activity was clearly revealed at pI 4.1. These results suggest that ECF of S. erinacei plerocercoid is an acidic glycoprotein. An intradermal injection of ECF eosinophil attractions in the normal guinea pig skin.
Horii
Y
Y
Department of Medical Zoology, Nagasaki University School of Medicine, Japan.
Owhashi
M
M
Ishii
A
A
Fujita
K
K
D000818:Animals; D002589:Cestoda / Q000032:analysis*; D002630:Chemotactic Factors / Q000032:analysis*; D002631:Chemotactic Factors, Eosinophil / Q000032:analysis* / Q000302:isolation & purification; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D006168:Guinea Pigs; D006358:Hot Temperature; D007525:Isoelectric Focusing; D008970:Molecular Weight; D010084:Oxidation-Reduction; D013146:Spirometra / Q000032:analysis*
D002630:Chemotactic Factors; D002631:Chemotactic Factors, Eosinophil