title: In vitro inhibition of lens epithelial cell growth by continuous wave Nd:YAG laser.
abstract: Bovine lens epithelial cells were suspended in MEM medium and subjected to continuous wave, low power, pulsed neodymium:yttrium-aluminum-garnet (Nd:YAG) laser irradiation. The temperature of each suspension was maintained at 36 degrees C. Laser applications ranged from 1 to 10 watts and from 100 to 2000 seconds, but the total dose to each of the epithelial cell suspension was 2000 J. Six to thirty-nine percent of the cells were dead immediately after irradiation. Surviving cells, cultured for 15 days, showed decreased attachment and failed to grow. These preliminary results suggest that the Nd:YAG laser may be used during cataract surgery to prevent subsequent lens epithelial cell proliferation and the resulting vision reduction and glare.
authors.affiliation: Shohzankai Medical Foundation, Miyake Eye Hospital, Nagoya, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Miyake
authors.forename: S
authors.initials: S
authors.lastname: Iwata
authors.forename: F
authors.initials: F
authors.lastname: Ando
authors.forename: N
authors.initials: N
authors.lastname: Daikuzono
authors.forename: J L
authors.initials: JL
authors.lastname: Federman
mesh_terms: D000818:Animals; D002455:Cell Division / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D004847:Epithelial Cells; D007834:Lasers; D007908:Lens, Crystalline / Q000166:cytology*