Regulation of rat growth hormone receptor gene expression.
A cDNA encoding the growth hormone (GH) receptor was cloned from rat liver. Both the nucleotide and translated amino acid sequence share greater than 70% similarity with the GH receptors from rabbit and human. An RNA probe was generated from this sequence for use in a solution hybridization assay to quantitate GH receptor mRNA expression in rat tissues. Expression was detected in 9/12 tissues examined, with the highest levels observed in the liver. Expression in liver, kidney, heart and muscle was developmentally regulated, being low at birth and rising to adult levels in 5 weeks. No difference was observed between hepatic expression in males and females, although livers from pregnant rats had elevated levels. Hypophysectomy and GH treatment did not affect hepatic GH receptor mRNA levels.
Mathews
L S
LS
Center for Biotechnology, Huddinge University Hospital, Sweden.
Enberg
B
B
Norstedt
G
G
D000375:Aging / Q000502:physiology*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005260:Female; D005786:Gene Expression Regulation; D005796:Genes; D007016:Hypophysectomy; D008099:Liver / Q000254:growth & development / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009928:Organ Specificity; D051381:Rats; D011919:Rats, Inbred Strains; D011986:Receptors, Somatotropin / Q000235:genetics*; D012016:Reference Values; D015183:Restriction Mapping; D012737:Sex Factors; D014158:Transcription, Genetic
D011986:Receptors, Somatotropin