Expression of transforming growth factor alpha and epidermal growth factor receptor messenger RNA in neoplastic and nonneoplastic human kidney tissue.
Using Northern blot analysis, we have demonstrated that mRNA for transforming growth factor alpha (TGF-alpha) was expressed in five malignant kidney tissue specimens but was not detected in their autologous nonneoplastic homologues. In addition, the expression of epidermal growth factor (EGF) receptor mRNA in these malignant tissues was 2- to 3-fold greater than in nontransformed tissues. In two cases examined using immunohistochemistry, we were able to correlate the increased expression of the mRNA with an increase in protein expression. Since TGF-alpha is known to bind to the EGF receptor, the finding of an increased expression of both TGF-alpha and EGF receptor mRNA in kidney tumor tissue suggests that interaction between TGF-alpha and the EGF receptor may play a role in promoting transformation and/or proliferation of kidney neoplasms, perhaps by an autocrine mechanism.
Mydlo
J H
JH
Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, New York 10021.
Michaeli
J
J
Cordon-Cardo
C
C
Goldenberg
A S
AS
Heston
W D
WD
Fair
W R
WR
D015152:Blotting, Northern; D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D066246:ErbB Receptors / Q000235:genetics*; D006801:Humans; D007668:Kidney / Q000378:metabolism*; D007680:Kidney Neoplasms / Q000235:genetics* / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics*; D014158:Transcription, Genetic; D015291:Transforming Growth Factors / Q000378:metabolism*
D012333:RNA, Messenger; D015291:Transforming Growth Factors; D066246:ErbB Receptors