Stimulation of human synovial fibroblast DNA synthesis by platelet-derived growth factor and fibroblast growth factor. Differences to the activation by IL-1.
The pronounced synovial hyperplasia often found in the joints of patients with rheumatoid arthritis could be explained partially by the action of monocyte-macrophage polypeptides (monokines). This report demonstrates that two cytokines which may be derived from monocyte-macrophage populations, namely platelet-derived growth factor (PDGF) and basic fibroblast growth factor (bFGF), stimulate the DNA synthesis and proliferation of human synovial fibroblast-like cells cultured in low (i.e., 1%) fetal bovine serum. Epidermal growth factor, insulin-like growth factor-I, insulin-like growth factor-II (multiplication stimulating activity) and substance P were inactive. Unlike IL-1, PDGF and FGF do not also stimulate PGE2, plasminogen activator, and hyaluronic acid levels. Thus PDGF and FGF, arising from stimulated monocyte-macrophages, may play a role in the stimulation of mesenchymal cell proliferation that often accompanies chronic inflammatory arthritic disease. The synovial cells respond to a variety of cytokines in different ways suggesting multiple-signaling pathways.
Butler
D M
DM
Department of Medicine, University of Melbourne, Parkville, Australia.
Leizer
T
T
Hamilton
J A
JA
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D005347:Fibroblasts / Q000378:metabolism* / Q000502:physiology; D005938:Glucocorticoids / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D006820:Hyaluronic Acid / Q000096:biosynthesis; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D011453:Prostaglandins / Q000502:physiology; D013583:Synovial Membrane / Q000378:metabolism*
D005938:Glucocorticoids; D006133:Growth Substances; D010982:Platelet-Derived Growth Factor; D011453:Prostaglandins; D005346:Fibroblast Growth Factors; D006820:Hyaluronic Acid; D004247:DNA