[Cellular localization, half-life, and secretion of peptide YY].
Tissue and plasma concentration of peptide YY (PYY) were measured by means of a radioimmunoassay (RIA) developed in our laboratory, using a specific PYY antiserum generated in New Zealand white rabbits against synthetic PYY, and dextran-coated charcoal to terminate the assay. Cellular localization of PYY was studied immunohistochemically using the peroxidase-antiperoxidase (PAP) technique. The highest tissue concentration of PYY was found in the mucosa of the terminal ileum and colon. PYY-containing secretory granules were primarily found in the basal pole of open-type endocrine cells. Basal plasma concentration of PYY was 70 +/- 9 pg/ml and rose to 357 +/- 30 pg/ml during the IV administration of PYY at 400 pmol/kg-h. A significant correlation was found (r = 0.94, p less than 0.05) between dose of PYY (12.5, 25, 50, 100, 200, 400 pmol/kg-h, IV) and plasma concentration of PYY. The calculated half-life of PYY in plasma was 8.3 +/- 1.9 minutes. Plasma concentration of PYY during the intraduodenal administration of sodium oleate (150 +/- 20 pg/ml) or long-chain triglyceride (187 +/- 37 pg/ml) was similar to plasma concentration of PYY obtained during the IV administration of PYY at 100 pmol/kg-h. Plasma concentration of PYY raised (126 +/- 10 pg/ml) after the administration of bombesin (400 pmol/kg-h, IV). Bile enhanced release of PYY. The present study suggests a hormonal role for PYY.
Lluis
F
F
Department of Surgery, University of Texas Medical Branch, Galveston.
Fujimura
M
M
Gómez
G
G
Salvá
J A
JA
Greeley
G H
GH
Thompson
J C
JC
D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D001646:Bile / Q000502:physiology; D001839:Bombesin / Q000494:pharmacology; D004064:Digestive System / Q000032:analysis*; D004285:Dogs; D005260:Female; D006207:Half-Life; D006851:Hydrochloric Acid / Q000494:pharmacology; D007124:Immunoenzyme Techniques; D008297:Male; D019301:Oleic Acid; D009829:Oleic Acids / Q000494:pharmacology; D019894:Peptide YY; D010455:Peptides / Q000032:analysis* / Q000378:metabolism / Q000493:pharmacokinetics; D012636:Secretory Rate / Q000187:drug effects; D014280:Triglycerides / Q000494:pharmacology
D000596:Amino Acids; D009829:Oleic Acids; D010455:Peptides; D014280:Triglycerides; D019894:Peptide YY; D019301:Oleic Acid; D001839:Bombesin; D006851:Hydrochloric Acid
Localización celular, vida media y secreción del péptido YY.