Normal ranges of the activities of ten different enzymes in 100 independent preparations of chorionic villi. Comparison of specimens from induced abortions, biopsies, and cultured cells.
In order to obtain a large set of normal control values, the activities of three cytosolic enzymes of purine metabolism and seven lysosomal enzymes were determined in homogenates of chorionic villi derived from induced abortions of normal pregnancies (7th-12th week) in about 100 individual cases. Possible reasons for the rather wide ranges of normal distributions of enzyme activities are discussed. The values are compared: (1) with available data in the literature; (2) with activities determined in decidual homogenates prepared from the same samples; (3) with activities of cells of cultures established and grown from villi in the same samples; and (4) with enzyme activities measured in chorionic biopsies using the same methods. Implications for the prenatal diagnosis of the associated metabolic diseases are considered.
Burgemeister
R
R
Institut für Anthropoligie und Humangenetik, Universität München, F.R.G.
Gutensohn
W
W
D000028:Abortion, Induced; D001706:Biopsy; D002478:Cells, Cultured; D002824:Chorionic Villi / Q000166:cytology / Q000201:enzymology* / Q000473:pathology; D005260:Female; D006801:Humans; D011247:Pregnancy; D012016:Reference Values