The role of cis-acting promoter elements in tissue-specific albumin gene expression.
The mouse albumin gene promoter has six closely spaced binding sites for nuclear proteins that are located between the TATA motif and nucleotide position -170. In vitro transcription with liver or spleen nuclear extracts of templates containing either mutated or polymerized albumin promoter elements establishes a hierarchy of the different protein binding sites for tissue-specific albumin gene transcription. The HNF-1 and C/EBP binding sites strongly activate transcription in a tissue-specific manner. The NF-Y binding site has a lower activation potential and is less specific, being equally efficient in liver and spleen nuclear extracts. The remaining elements are relatively weak activator sites.
Maire
P
P
Département de Biologie Moléculaire, Sciences II, Geneva, Switzerland.
Wuarin
J
J
Schibler
U
U
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D022762:CCAAT-Enhancer-Binding Proteins; D002352:Carrier Proteins / Q000378:metabolism / Q000494:pharmacology; D002467:Cell Nucleus / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D029382:Dicarboxylic Acid Transporters; D005786:Gene Expression Regulation / Q000187:drug effects; D008099:Liver / Q000378:metabolism / Q000648:ultrastructure; D051379:Mice; D009687:Nuclear Proteins / Q000378:metabolism / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D012709:Serum Albumin / Q000235:genetics*; D013154:Spleen / Q000378:metabolism / Q000648:ultrastructure; D013698:Templates, Genetic; D014157:Transcription Factors; D014158:Transcription, Genetic / Q000187:drug effects
D022762:CCAAT-Enhancer-Binding Proteins; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D029382:Dicarboxylic Acid Transporters; D009687:Nuclear Proteins; D012709:Serum Albumin; D014157:Transcription Factors