Platelet adhesion onto protein-coated and uncoated polyetherurethaneurea having tertiary amino groups in the substituents and its derivatives.
Interactions of platelet with novel polyetherurethaneurea and its heparinized derivative were investigated. Platelet adhesion onto the material and release of serotonin or adenosine phosphate from platelet-rich plasma (PRP) were suppressed by an introduction of amino groups to polyetherurethaneurea, by quaternization of the polymer, and further by heparinization of the polymer. When the material was precoated with one of major plasma proteins and the protein-coated materials were taken to contact with washed platelet suspension (WP), the dependence of platelet adhesion and activation on the properties of polymers was different from that observed for PRP interaction. Platelet adhesion and activation were promoted according to the nature of coating proteins in the order albumin less than gamma-globulin less than fibrinogen and with increasing degree of denaturation of coating proteins. When the polymer materials were coated with proteins by immersing in aqueous solution containing two kinds of plasma proteins, adhesion behaviors of platelet were similar to those observed for PRP-uncoated material interaction. These experimental facts indicate that the selectivity of platelet for protein-coated material cannot be assessed by the interaction of WP with materials coated with a single kind of protein. It was concluded that material surface to which albumin is selectively adsorbed without denaturation does not stimulate adhering platelets for release reactions.
Ito
Y
Y
Department of Polymer Chemistry, Faculty of Engineering, Kyoto University, Japan.
Sisido
M
M
Imanishi
Y
Y
D000818:Animals; D001672:Biocompatible Materials; D001798:Blood Proteins; D001807:Blood Vessel Prosthesis; D004285:Dogs; D066298:In Vitro Techniques; D010973:Platelet Adhesiveness; D011140:Polyurethanes; D013816:Thermodynamics
D001672:Biocompatible Materials; D001798:Blood Proteins; D011140:Polyurethanes; C044690:polyetherurethane urea