title: Analysis of the promoter region of the cloned kanamycin resistance gene (kmr) from Streptomyces kanamyceticus.
abstract: The appropriate location and orientation of the kanamycin resistance gene (kmr) cloned on multicopy plasmids were determined by subcloning experiments. The transcription start site was identified by high-resolution S1 mapping and the kmr mRNA was shown to have a long leader of about 400 bp. An additional transcript upstream of the kmr gene was also detected, which ran in the opposite direction and overlapped 2 to 3 nucleotides with the kmr transcript. The presumptive promoter region of this physiologically unidentified RNA was similar to the Escherichia coli promoter consensus sequence both in the -10 and -35 regions, whereas the putative promoter region of the kmr gene exhibited sequence similarities in the -10 region to the promoters of the endoglycosidase H (endoH) and the viomycin phosphotransferase (vph) genes from Streptomyces.
authors.affiliation: Department of Biochemistry, Meiji College of Pharmacy, Tokyo, Japan.
authors.forename: M M
authors.initials: MM
authors.lastname: Nakano
authors.forename: T
authors.initials: T
authors.lastname: Kikuchi
authors.forename: H
authors.initials: H
authors.lastname: Mashiko
authors.forename: H
authors.initials: H
authors.lastname: Ogawara
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D004352:Drug Resistance, Microbial / Q000235:genetics*; D007612:Kanamycin / Q000494:pharmacology*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D013302:Streptomyces / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D007612:Kanamycin