In vitro cytotoxicity in adult Xenopus generated against larval targets and minor histocompatibility antigens.
Our experiments reveal that application of several minor H antigen-disparate skin grafts to adult Xenopus over an 18-month period can lead to in vitro generation of CML reactivity toward these minor antigens. Furthermore, we demonstrate that, following MHC-disparate skin graft rejection, adult effectors can efficiently kill both adult and larval donor-strain targets; this killing is MHC-specific and requires MLC restimulation with cells syngeneic to the skin graft donor. The ability to kill larval lymphoblasts, which have been shown elsewhere to be MHC class I-negative but class II-positive, suggests the probable importance of class II-restricted killing in this species.
Horton
T L
TL
Department of Biological Sciences, University of Durham, United Kingdom.
Horton
J D
JD
Varley
C A
CA
D000818:Animals; D003602:Cytotoxicity, Immunologic; D006649:Histocompatibility Antigens / Q000276:immunology*; D066298:In Vitro Techniques; D007814:Larva / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D014982:Xenopus laevis / Q000276:immunology*
D006649:Histocompatibility Antigens