title: Generation and selection of monoclonal antibodies identifying surface molecules discriminating between fetal and adult fibroblasts.
abstract: The present report describes the reactivity of two murine monoclonal antibodies, i.e., CB11 and CB43, the first obtained by using embryonic material as immunizer and the second elicited against a human cell line. These reagents recognize two distinct molecules marking a peculiar differentiation stage on fetal/embryonic fibroblasts. The first structure is a single chain of 14.5 kd, the second one a heterodimer of 40-80 kd. Even if these reagents are not strictly fibroblast restricted, translineage expression does not diminish the medical relevance of the reagents; in fact, they may have application in prenatal diagnostics and amniocentesis testing, in which it is necessary to discriminate between fetal and maternal cells.
authors.affiliation: Dipartimento di Genetica, Biologia e Chimica Medica, Università di Torino, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Funaro
authors.forename: G
authors.initials: G
authors.lastname: Bellone
authors.forename: L
authors.initials: L
authors.lastname: DeMonte
authors.forename: M
authors.initials: M
authors.lastname: Alessio
authors.forename: S
authors.initials: S
authors.lastname: Roggero
authors.forename: L
authors.initials: L
authors.lastname: Peruzzi
authors.forename: A P
authors.initials: AP
authors.lastname: Cappa
authors.forename: F
authors.initials: F
authors.lastname: Malavasi
mesh_terms: D000328:Adult; D000649:Amniocentesis; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D002454:Cell Differentiation; D002460:Cell Line; D005333:Fetus / Q000276:immunology; D005347:Fibroblasts / Q000166:cytology / Q000276:immunology*; D006801:Humans; D011296:Prenatal Diagnosis; D014407:Tumor Cells, Cultured / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface