title: Transsynaptic degeneration of motoneurones caudal to spinal cord lesions.
abstract: We studied the effects of complete transversal section of the spinal cord, at T8-10, in adult rats, upon the number and morphology of identified motoneurones in lumbar segments L4 and L5. In observations by light and electron microscopy many lumbar motoneurones had structural abnormalities when the interval between surgery and perfusion ranged between a few hours and one week. We found also that as many as 25% of the motoneurones distal to a cord transection disappeared as a consequence of the lesions. We did not find comparable changes in the spinal cord at C6 after transection at T8-10. Complete removal of the cerebellum did not reduce the lumbar motoneurone counts. Bilateral ablation of the "motor" cortex did cause a reduction of motoneurone counts at L4-5; these animals showed normal or near normal spontaneous locomotor activity beginning a few days after the lesion was placed. Motoneurone counts were significantly reduced after partial cord lesions that spared the dorsal funiculi (where the corticospinal tract travels in the rat), but in this case the rats were paraplegic as a result of the lesion. Cord transection at 7 days of postnatal age resulted in reduced motoneurone counts when the rats reached adulthood. Intraspinal or subarachnoid administration of colchicine led to reduced motoneurone counts. Prolonged infusion of a GABA agonist, muscimol, into the lumbar CSF did not prevent the loss of motoneurones produced by cord transection. Pretreatment of animals with a Ca2+ channel blocker (nimodipine) did not prevent the effects of cord transection.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Division of Neurosurgery, Audie L. Murphy Veterans Administration Hospital, San Antonio, TX 78284.
authors.forename: E
authors.initials: E
authors.lastname: Eidelberg
authors.forename: L H
authors.initials: LH
authors.lastname: Nguyen
authors.forename: R
authors.initials: R
authors.lastname: Polich
authors.forename: J G
authors.initials: JG
authors.lastname: Walden
mesh_terms: D000818:Animals; D003078:Colchicine / Q000494:pharmacology; D008297:Male; D009046:Motor Neurons / Q000187:drug effects / Q000502:physiology*; D009118:Muscimol / Q000494:pharmacology; D009410:Nerve Degeneration / Q000187:drug effects*; D009627:Nomifensine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013119:Spinal Cord Injuries / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology
chemical_list: D009627:Nomifensine; D009118:Muscimol; D003078:Colchicine