Identification of the heat-labile hemolysin of Actinobacillus pleuropneumoniae serotype 1.
The heat-labile hemolysin of Actinobacillus pleuropneumoniae serotype 1 strain CM-5 was partially purified using ammonium sulfate precipitation and gel permeation chromatography. This partially purified material was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and blotted onto nylon filters. The filters were treated with convalescent pig serum and subsequently with CM-5 culture supernatant containing active hemolysin. A 104 kd peptide was identified as the hemolysin because it bound antibodies in convalescent pig serum which cross-linked active hemolysin. The same 104 kd protein when injected into a rabbit produced neutralizing antibodies to the CM-5 hemolysin in culture supernatant.
Devenish
J
J
Department of Veterinary Microbiology and Immunology, Ontario Veterinary College, University of Guelph.
Rosendal
S
S
D000188:Actinobacillus / Q000032:analysis* / Q000145:classification; D000818:Animals; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D006460:Hemolysin Proteins / Q000032:analysis* / Q000276:immunology; D012703:Serotyping; D013045:Species Specificity
D006460:Hemolysin Proteins