Hyperoxic exposure alters gene expression in the lung. Induction of the tissue inhibitor of metalloproteinases mRNA and other mRNAs.
Exposure to high concentrations of oxygen can result in tissue damage, particularly in the lung. Lung pathology induced by hyperoxia includes changes in lung cell populations and morphology. Presumably, alterations in gene expression underlie some of these cellular changes. In order to better understand the molecular basis of these events, a cDNA library was constructed from the mRNA of the lungs of a hyperoxia-exposed rabbit and differentially screened for clones corresponding to hyperoxia-induced messages. This approach has led to the isolation of four clones, three of which are presented in this communication. One clone corresponds to a message whose steady state levels were induced 6-fold and encodes the tissue inhibitor of metalloproteinases, a protein that plays a key role in the regulation of connective tissue turnover in some cells and potentiates erythroid development in others.
Horowitz
S
S
Department of Pediatrics (Neonatology), University of Rochester Medical Center, New York 14642.
Dafni
N
N
Shapiro
D L
DL
Holm
B A
BA
Notter
R H
RH
Quible
D J
DJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004791:Enzyme Inhibitors / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects; D008168:Lung / Q000502:physiology*; D008969:Molecular Sequence Data; D010100:Oxygen / Q000494:pharmacology*; D011817:Rabbits; D019714:Tissue Inhibitor of Metalloproteinases
D004791:Enzyme Inhibitors; D019714:Tissue Inhibitor of Metalloproteinases; D004247:DNA; D010100:Oxygen