title: The triose phosphate-3-phosphoglycerate-phosphate translocator from spinach chloroplasts: nucleotide sequence of a full-length cDNA clone and import of the in vitro synthesized precursor protein into chloroplasts.
abstract: The nucleotide sequence of several cDNA clones coding for the phosphate translocator from spinach chloroplasts has been determined. The cDNA clones were selected from a lambda gt10 library prepared from poly(A)+ mRNA of spinach leaves using oligonucleotide probes modeled from amino acid sequences of tryptic peptides prepared from the isolated translocator protein. A 1439 bp insert of one of the clones codes for the entire 404 amino acid residues of the precursor protein corresponding to a mol. wt of 44,234. The full-length clone includes 21 bp at the transcribed non-coding 5' region with the ribosome initiation sequence ACAATGG, a 1212 bp coding region and 199 bp at the non-coding 3' region excluding the poly(A) tail which starts 17 bp downstream from a putative polyadenylation signal, AATAAT. According to secondary structure predictions the mature part of the chloroplast phosphate translocator exhibits high hydrophobicity and consists of at least seven membrane-spanning segments. Using plasmid-programmed wheat germ lysate the precursor protein was synthesized in vitro and could be imported into spinach chloroplasts where it is inserted into the inner envelope membrane.
authors.affiliation: Institut für Biochemie der Pflanze, Göttingen, FRG.
authors.forename: U I
authors.initials: UI
authors.lastname: Flügge
authors.forename: K
authors.initials: K
authors.lastname: Fischer
authors.forename: A
authors.initials: A
authors.lastname: Gross
authors.forename: W
authors.initials: W
authors.lastname: Sebald
authors.forename: F
authors.initials: F
authors.lastname: Lottspeich
authors.forename: C
authors.initials: C
authors.lastname: Eckerskorn
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D060365:Chloroplast Proteins; D002736:Chloroplasts / Q000378:metabolism*; D004247:DNA / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D010710:Phosphates / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D010944:Plants / Q000235:genetics* / Q000378:metabolism; D011498:Protein Precursors / Q000378:metabolism; D011499:Protein Processing, Post-Translational
chemical_list: D060365:Chloroplast Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D010710:Phosphates; D010940:Plant Proteins; D011498:Protein Precursors; D004247:DNA