Monoclonal antibody MACG1 distinguishes between different molecular species of the ganglioside GM3.
The present study investigates the chemical structure of a ganglioside, detected by monoclonal antibody (MAb) MacG1, which reacts with intracytoplasmic granules of tumor-infiltrating macrophages. The results obtained by enzymatic hydrolysis and fast-atom bombardment-mass spectrometry reveal that MAb MacG1 reacts with a subcomponent of the ganglioside GM3 found in melanoma and bovine brain. MAb MacG1 might be a powerful tool to distinguish among GM3 species and could help to define their possibly different biological functions.
Schriever
F
F
Institute of Immunology, University of Munich, West Germany.
Dennis
R D
RD
Pallmann
B
B
Riethmüller
G
G
Johnson
J P
JP
D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens; D001921:Brain / Q000378:metabolism; D002417:Cattle; D005679:G(M3) Ganglioside / Q000145:classification / Q000276:immunology* / Q000378:metabolism; D005732:Gangliosides / Q000276:immunology*; D006801:Humans; D008545:Melanoma / Q000378:metabolism; D015394:Molecular Structure; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D000941:Antigens; D005679:G(M3) Ganglioside; D005732:Gangliosides