Strain differentiation of barley yellow dwarf virus isolates using specific monoclonal antibodies in immunosorbent electron microscopy.
Three strain-specific monoclonal antibodies, MAC. 91, MAC. 92 and MAb. MAFF2, were compared with two polyclonal antisera for the diagnosis of barley yellow dwarf virus (BYDV) strains in immunosorbent electron microscopy (ISEM). Used at 1 micrograms ml-1, the three monoclonal antibodies specifically trapped the Rothamsted PAV, RPV and MAV-like type isolates respectively, unlike the polyclonal antisera, which showed considerable heterologous reaction. The efficiency of the monoclonal and polyclonal antibodies in trapping and detecting these isolates of BYDV were compared quantitatively using ISEM and enzyme-linked immunosorbent assay (ELISA) techniques. The test provides a simple and sensitive diagnostic method for BYDV, suitable for small numbers of samples and is useful in confirming doubtful ELISA results.
Forde
S M
SM
AFRC, Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Herts., U.K.
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000276:immunology; D002523:Edible Grain / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D001467:Hordeum / Q000382:microbiology*; D008854:Microscopy, Electron / Q000379:methods; D010936:Plant Extracts; D010942:Plant Viruses / Q000145:classification / Q000302:isolation & purification*; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D010936:Plant Extracts