Determination of triacylglycerol lipase activity using carbon-13-labeled triacylglycerols and nuclear magnetic resonance spectroscopy: evidence that hepatic lipase hydrolyzes medium-chain triacylglycerols.
Triacylglycerol lipase activity was studied using glycerol [1-13C]trioctanoate mixed with postheparin rat plasma. 13C NMR spectroscopy demonstrated triacylglycerol hydrolysis into free fatty acids with no difference at the 1,3 or 2 glycerol positions. There was no inhibition by high sodium concentration, consistent with lipase of hepatic origin.
Dimand
R J
RJ
Department of Pediatrics, University of California, Davis 95616.
Bradbury
E M
EM
Cox
K L
KL
D000818:Animals; D002247:Carbon Isotopes; D005230:Fatty Acids, Nonesterified / Q000378:metabolism*; D006493:Heparin / Q000494:pharmacology; D008049:Lipase / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D009682:Magnetic Resonance Spectroscopy; D051381:Rats; D011919:Rats, Inbred Strains; D012965:Sodium Chloride / Q000494:pharmacology; D014280:Triglycerides / Q000378:metabolism
D002247:Carbon Isotopes; D005230:Fatty Acids, Nonesterified; D014280:Triglycerides; D012965:Sodium Chloride; D006493:Heparin; D008049:Lipase