Efficient primary isolation of human immunodeficiency virus.
A highly-sensitive and efficient culture technique for human immunodeficiency virus type-1 (HIV-1) is described; HIV-1 was recovered from the lymphocytes of 44 (94%) antibody-seropositive healthy or symptomatic individuals. The reductions in the requirements for both the reagent volume and the number of patients' lymphocytes, together with an increased efficiency, has made this HIV-1 culture system more practical for diagnostic virology laboratories.
Pietroboni
G R
GR
Virus Laboratory, Combined Microbiology Service, Queen Elizabeth II Medical Centre, Nedlands, WA.
Harnett
G B
GB
Bucens
M R
MR
D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology; D001782:Blood Donors; D002478:Cells, Cultured; D004526:Efficiency; D006679:HIV Seropositivity / Q000097:blood / Q000382:microbiology; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D008214:Lymphocytes / Q000382:microbiology*; D014776:Virus Cultivation