Neuroblastoma double minutes isolated by pulsed-field gel electrophoresis without prior strand-cleaving treatments.
In a human neuroblastoma line, minute chromosomes were separable from the bulk of interphase nuclear DNA by contour-clamped homogeneous electric field (CHEF) gel electrophoresis. The minute chromosomes showed a homogeneous size of approximately 3 Mbp and contained amplified N-myc genes. Fractionation was accomplished without prior strand-cleaving treatment of the DNA, indicating that at least a portion of the minute chromosomes exist as free entities in the interphase nuclei. Human alphoid satellite DNA sequences were also detected in the 3-Mbp band. It is possible that alphoid sequences are contained in the constricted central region that joins these double minutes.
Chen
T L
TL
Section of Neuropathology, Yale University School of Medicine, New Haven, Connecticut 06511.
Manuelidis
L
L
D004273:DNA, Neoplasm / Q000302:isolation & purification*; D004276:DNA, Satellite / Q000032:analysis; D004586:Electrophoresis / Q000379:methods*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D005784:Gene Amplification; D006801:Humans; D009447:Neuroblastoma / Q000235:genetics* / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000648:ultrastructure
D004273:DNA, Neoplasm; D004276:DNA, Satellite