title: Synthesis of the dodecapeptide corresponding to domain III of bovine brain calmodulin: alpha-beta shift side reactions during the synthesis by the classical method in solution.
abstract: We report details of the chemical synthesis of the dodecapeptide corresponding to the calcium binding loop III of bovine brain calmodulin (sequence 93-104) and its fragments 96-04, 93-98, and 99-104. The preparation of the peptides employed classical solution methods and a fragment-condensation strategy. The major difficulties were encountered during the synthesis of the peptides containing the N-terminal sequences -Gly-Asn-Gly- and -Asp-Lys-Asp-Gly-Ans-Gly-, in which alpha-beta shift side reactions were observed.
authors.forename: G
authors.initials: G
authors.lastname: Borin
authors.forename: A
authors.initials: A
authors.lastname: Calderan
authors.forename: P
authors.initials: P
authors.lastname: Ruzza
authors.forename: A
authors.initials: A
authors.lastname: Pezzoli
authors.forename: F
authors.initials: F
authors.lastname: Marchiori
authors.forename: E
authors.initials: E
authors.lastname: Peggion
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism; D002147:Calmodulin / Q000138:chemical synthesis*; D002417:Cattle; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis*
chemical_list: D002147:Calmodulin; D007202:Indicators and Reagents; D010455:Peptides