Program in BASIC for Ferguson plot analysis, using a personal computer: application to gel electrophoresis in a continuous buffer.
A program in BASIC suitable for personal computers is described which is applicable to gel electrophoresis conducted in a single (continuous) buffer. The curve fitting is to a polynomial function, allowing for an objective selection of the most appropriate curve type and order--linear, convex or concave--in the particular application. Results do not differ significantly from previous programs for evaluation of linear Ferguson plots or of curve fitting to an exponential function for evaluating convex plots, executed on mainframe computers such as the DEC-10 (Digital) and IBM 370 computers. Thus, the program combines original versatility with, for the first time, the possibility for widespread application of Ferguson plot analysis on personal computers.
Gombocz
E
E
Section on Macromolecular Analysis, National Institute of Child Health and Human Development, Bethesda, MD 20892.
Chrambach
A
A
D001797:Blood Protein Electrophoresis / Q000379:methods*; D003627:Data Interpretation, Statistical; D004563:Electrochemistry; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D008838:Microcomputers; D010316:Particle Size; D011381:Programming Languages; D012984:Software