Compartmental biodistribution of a monoclonal antibody against human lung adenocarcinoma grown in athymic mice.
The biodistribution of the radioiodinated monoclonal antibody KS1/4 and of its F(ab) and F(ab')2 fragments were studied in tumor-bearing nude mice. Intravenous administration of the intact antibody resulted in the highest tumor retention and tumor/blood ratios. No metabolites or antigen-antibody conjugates were detectable, in serum, by HPLC analyses. Reasonable correlations were obtained between the estimated time-course of KS1/4 and experimental biodistribution data using an eight-compartment linear radiopharmacokinetic model, suggesting that such models may be useful in predicting the biodistribution and effective targeting of this monoclonal antibody.
Shani
J
J
Radiopharmacy Program, School of Pharmacy, University of Southern California, Los Angeles 90033.
Mohd
S
S
Wolf
W
W
Walker
L E
LE
D000230:Adenocarcinoma / Q000000981:diagnostic imaging* / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000912:Antibodies, Neoplasm / Q000032:analysis*; D000936:Antigen-Antibody Complex / Q000032:analysis; D005260:Female; D008175:Lung Neoplasms / Q000000981:diagnostic imaging* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008954:Models, Biological; D009368:Neoplasm Transplantation; D011877:Radionuclide Imaging; D014018:Tissue Distribution; D014183:Transplantation, Heterologous
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D000936:Antigen-Antibody Complex