[Flow cytometry in immunology and hematology: some essential practical aspects].
Flow cytometry, supported by monoclonal antibodies, has widely contributed in the cellular identification, notably in the clinical and haematological fields. This technique has found several applications since the qualitative phenotyping and the quantitative analysis of the immune system's cell populations are helpful in the diagnosis and the therapy. Besides, these indications require an appropriate knowledge of several methodological aspects including factors related to the sample donor: age and sex; sampling: time and quantity; and the sample preparation conditions. References values, needed for the results interpretation, have a meaning only if they are defined within these validity limits. Previous trials have been done in order to define a biological value representative of the immunological status, such as the CD4/CD8 ratio. Unfortunately this ratio is not justified in the scope of new knowledge concerning the cellular interactions and the functional heterogeneity of cells involved in the immune system.
Doinel
C
C
INSERM U 76, INTS, Paris.
Bourin
P
P
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000032:analysis; D002452:Cell Count; D002469:Cell Separation / Q000379:methods; D005434:Flow Cytometry; D006405:Hematology / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007158:Immunologic Techniques / Q000295:instrumentation; D012015:Reference Standards
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation
La cytométrie en flux en immunologie et en hématologie: quelques aspects pratiques essentiels.