Studies on the mechanism of action of the alkaline phosphatase from Dictyostelium discoideum.
The Dictyostelium discoideum alkaline phosphatase was investigated kinetically in an attempt to elucidate its mechanism of action. Analysis of the hydrolysis of p-nitrophenyl phosphate by stopped-flow spectrophotometry revealed biphasic kinetics, suggesting a double displacement enzyme mechanism. Furthermore, Tris stimulated activity in an uncompetitive manner, a result that was consistent with this interpretation. The enzyme was inhibited reversibly by phosphate at low ionic strength, but the inhibition was irreversible at high ionic strength and the latter effect was enhanced at alkaline pH values. These results indicate that high ionic strength and alkaline pH conditions bring about a conformational change that renders the enzyme susceptible to irreversible inhibition by phosphate.
Bhanot
P
P
Department of Microbiology, University of British Columbia, Vancouver, Canada.
Weeks
G
G
D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D001667:Binding, Competitive; D004023:Dictyostelium / Q000201:enzymology*; D007700:Kinetics; D009596:Nitrophenols; D009943:Organophosphorus Compounds; D010710:Phosphates / Q000494:pharmacology; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D014325:Tromethamine
D009596:Nitrophenols; D009943:Organophosphorus Compounds; D010710:Phosphates; D014325:Tromethamine; C008644:nitrophenylphosphate; D000469:Alkaline Phosphatase