Gene transfer and expression studies in cultured avian neural crest cells differentiating into melanocytes.
Neural crest cells obtained from explanted neural tubes take up, express, and retain exogenous DNA applied by the CaPO4 co-precipitation method during their differentiation into melanocytes. High efficiencies of gene transfer were obtained with both supercoiled DNA and intact phage particles; linear DNA or DNA from the phage yielded very low efficiencies. There is some evidence that transferred gene expression is differentiation dependent. The system should be useful for studies concerned with the analysis of cell developmental genes and their regulatory elements.
Vielkind
J R
JR
British Columbia Cancer Research Centre, Canada.
Vogel
K S
KS
D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D003370:Coturnix; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D008544:Melanocytes / Q000166:cytology*; D009432:Neural Crest / Q000166:cytology*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D014162:Transfection
D004247:DNA; D015500:Chloramphenicol O-Acetyltransferase