title: Separation and quantification of serum alkaline phosphatase isozymes in the rat by affinity electrophoresis.
abstract: The purpose of this study was to examine the possibility of separation and quantification of serum alkaline phosphatase (ALP) isozymes in rats by wheatgerm lectin affinity electrophoresis. Cellulose acetate electrophoresis of the liver and bone ALPs without lectin results in overlapping bands, but in the presence of lectin, the mobility of the band of bone enzyme was retarded and well separated from the liver enzyme band. With this affinity electrophoretic method, we determined the serum ALP isozymes in fed and fasting rats grouped by age. As a result, the absolute activity of bone isozyme showed a downward trend with age in the fed and fasting rats. The serum ALP activity was steadily higher in fed rats than in fasting rats, and the increase was due to intestinal ALP isozyme. There was low activity bordering complete absence in liver isozyme under both nutritional conditions. The affinity electrophoretic method provided a rapid, reproducible, and relatively simple technique for further clinical characterization of ALP isozyme in the rat serum.
authors.affiliation: Preclinical Research Laboratories, Central Institute for Experimental Animals, Kawasaki, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Unakami
authors.forename: M
authors.initials: M
authors.lastname: Hirata
authors.forename: K
authors.initials: K
authors.lastname: Ichinohe
authors.forename: Y
authors.initials: Y
authors.lastname: Tanimoto
authors.forename: H
authors.initials: H
authors.lastname: Iizuka
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood / Q000302:isolation & purification*; D000818:Animals; D004586:Electrophoresis / Q000379:methods*; D007527:Isoenzymes / Q000097:blood / Q000302:isolation & purification*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D007527:Isoenzymes; D000469:Alkaline Phosphatase