[Transforming potential of DNA from solid tumor of human gastric carcinoma].
The NIH-3T3 and Rat-1 recipient cells were transfected with DNA from solid tumor or metastatic tissue of 8 gastric carcinoma patients by the method of calcium phosphate precipitation or G418 co-transfection. Seven of them were shown to have transforming potential. After the third round of transfection, a series of transformants were obtained which showed malignant phenotype, growth ability in soft agar culture, tumorigenicity in nude mice and retaining of human Alu repeat sequence. Through Southern hybridization of DNA from the secondary transformant, H-ras oncogene was demonstrated with the same length as in the human cells.
Wu
C J
CJ
Beijing Institute for Cancer Research.
D000818:Animals; D002471:Cell Transformation, Neoplastic; D004273:DNA, Neoplasm / Q000235:genetics*; D005347:Fibroblasts; D011905:Genes, ras; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D013274:Stomach Neoplasms / Q000235:genetics*; D014162:Transfection / Q000235:genetics*; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm