title: Coat protein of potyviruses. 6. Amino acid sequences suggest watermelon mosaic virus 2 and soybean mosaic virus-N are strains of the same potyvirus.
abstract: The amino acid sequence of the coat protein of watermelon mosaic virus 2 (WMV 2) was determined by a combination of peptide and nucleic acid sequencing. The coat protein of WMV 2 contained 281 amino acid residues including a single cysteine at position 132 and a blocked amino terminus. Comparison with the coat protein sequences of 20 strains of ten distinct potyviruses showed sequence homologies ranging from 43% to 69% except for the N strain of soybean mosaic virus (SMV-N), where the sequence homology with WMV 2 was 83%. This degree of homology and the location of sequence differences between WMV 2 and SMV-N is much closer to that observed between strains of the same virus than that found between distinct potyviruses. These data suggest that WMV 2 and SMV-N may be strains of the same virus.
authors.affiliation: CSIRO, Division of Biotechnology, Parkville Laboratory, Victoria, Australia.
authors.forename: M H
authors.initials: MH
authors.lastname: Yu
authors.forename: M J
authors.initials: MJ
authors.lastname: Frenkel
authors.forename: N M
authors.initials: NM
authors.lastname: McKern
authors.forename: D D
authors.initials: DD
authors.lastname: Shukla
authors.forename: P M
authors.initials: PM
authors.lastname: Strike
authors.forename: C W
authors.initials: CW
authors.lastname: Ward
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002213:Capsid / Q000235:genetics*; D003001:Cloning, Molecular; D005638:Fruit / Q000382:microbiology; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000145:classification / Q000235:genetics*; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000235:genetics; D013025:Glycine max / Q000382:microbiology
chemical_list: D000596:Amino Acids; D009841:Oligonucleotides