Transforming growth factor alpha: an aromatic side chain at position 38 is essential for biological activity.
Site-directed mutagenesis has been performed in the human transforming growth factor alpha gene. When tyrosine 38 is mutated into phenylalanine or tryptophane, biological activity is retained. In contrast, other alterations between cysteine 34 and cysteine 43 and disruption of disulfide bonds 8 to 21 and 34 to 43 resulted in loss of activities. The presence of an aromatic side chain at position 38 of transforming growth factor alpha seems to be essential for its activity.
Lazar
E
E
Laboratory of Chemoprevention, National Cancer Institute, Bethesda, Maryland 20892.
Vicenzi
E
E
Van Obberghen-Schilling
E
E
Wolff
B
B
Dalton
S
S
Watanabe
S
S
Sporn
M B
MB
D000595:Amino Acid Sequence; D000818:Animals; D005075:Biological Evolution; D003001:Cloning, Molecular; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D013045:Species Specificity; D013329:Structure-Activity Relationship; D015291:Transforming Growth Factors / Q000235:genetics* / Q000502:physiology
D015291:Transforming Growth Factors