title: Evaluation of uncertainties for parameters in binding studies: the sum-of-squares profile and Monte Carlo estimation.
abstract: Methods for characterization and evaluation of uncertainties in the parameter values for binding experiments are presented. A sum-of-squares profile is defined and illustrated. Sum-of-squares profiles give a qualitative description of both the uncertainties and correlation of parameters. The Monte Carlo method is developed as an accurate means of evaluating uncertainties in parameter values for nonlinear models. Examples are given for both actual and synthetic data.
authors.affiliation: Laboratory of Biochemical Pharmacology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892.
authors.forename: H A
authors.initials: HA
authors.lastname: Saroff
mesh_terms: D001665:Binding Sites; D004798:Enzymes / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism*; D007700:Kinetics; D008024:Ligands; D008962:Models, Theoretical; D009010:Monte Carlo Method; D009874:Operations Research; D010108:Oxyhemoglobins / Q000378:metabolism; D011485:Protein Binding
chemical_list: D004798:Enzymes; D006454:Hemoglobins; D008024:Ligands; D010108:Oxyhemoglobins