Study of mitochondrial organization in living resident and activated macrophages using the laser dye rhodamine 123.
Rhodamine 123, a fluorescent laser dye that labels metabolically active mitochondria of living cells, was used to analyse the pattern of distribution of mitochondria in resident and activated mouse peritoneal macrophages kept in culture for 4 or 24 hr. Activated macrophages kept for 4 hr in culture showed abundant small mitochondria distributed throughout the cell. This pattern changes to a situation in which the mitochondria are filamentous and radiate from the perinuclear region when these cells are kept in culture for 24 hr, acquiring a pattern similar to that observed in resident macrophages. After treatment with phorbol myristate acetate, resident macrophages presented a mitochondrial distribution similar to that observed in activated macrophages.
de Carvalho
T U
TU
Laboratório de Microscopia Electrônica e Ultraestrutura Celular, Universidade Federal do Rio de Janeiro, Brasil.
de Souza
W
W
D000818:Animals; D008264:Macrophages / Q000648:ultrastructure*; D051379:Mice; D008815:Mice, Inbred Strains; D008856:Microscopy, Fluorescence / Q000379:methods; D008928:Mitochondria / Q000648:ultrastructure*; D020112:Rhodamine 123; D012235:Rhodamines
D012235:Rhodamines; D020112:Rhodamine 123