Identification of D-sorbitol 3-phosphate in the normal and diabetic mammalian lens.
The 31P NMR spectra of mammalian lenses show, in addition to previously identified compounds such as ATP, Pi, glycerophosphorylcholine, and alpha-glycerophosphate, an unusual phosphorylated metabolite resonating at 6.5 ppm. The concentration of this compound increases manyfold in the lenses of diabetic rats concomitant with activation of the aldose reductase pathway. We have purified this material and have identified it as D-sorbitol 3-phosphate. It appears likely that this unusual metabolite of sorbitol may reflect or be part of the metabolic aberration of diabetes in the lens.
Szwergold
B S
BS
Department of Nuclear Magnetic Resonance and Medical Spectroscopy, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
Kappler
F
F
Brown
T R
TR
Pfeffer
P
P
Osman
S F
SF
D000818:Animals; D002417:Cattle; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D006600:Hexosephosphates / Q000302:isolation & purification*; D007908:Lens, Crystalline / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D051381:Rats; D013012:Sorbitol / Q000378:metabolism*; D013329:Structure-Activity Relationship
D006600:Hexosephosphates; C059844:sorbitol 3-phosphate; D013012:Sorbitol