Low-density lipoprotein derived from atherosclerotic patients enhances macrophage cholesterol accumulation and in vitro platelet aggregation.
The aims of our study were to assess the differences between plasma lipoproteins separated from five angiographically normal subjects and five patients with proven CHD. The patients with CHD had significantly higher levels of LDL-cholesterol and apo-B, and reduced levels of HDL-cholesterol and apo-Al. The biological characteristics of LDL and HDL from both groups of patients demonstrated that the LDL from the CHD patients enhanced platelet aggregation and increased cholesterol content and cholesterol esterification in MPM compared to the normal patients. HDL had no significant effect on MPM; however, there was an increased platelet aggregation with HDL derived from the CHD patients, while the HDL from the normal group decreased platelet aggregation. The data suggest that lipoproteins isolated from CHD patients are more atherogenic than lipoproteins from normal patients.
Keidar
S
S
Lipid Research Unit, Rambam Medical Center Technion Haifa, Israel.
Aviram
M
M
Grenadier
E
E
Markiewicz
W
W
Brook
J G
JG
D000328:Adult; D000818:Animals; D001161:Arteriosclerosis / Q000097:blood*; D002784:Cholesterol / Q000097:blood*; D002788:Cholesterol Esters / Q000378:metabolism; D003327:Coronary Disease / Q000097:blood; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D010974:Platelet Aggregation / Q000187:drug effects*
D002788:Cholesterol Esters; D008077:Lipoproteins, LDL; D002784:Cholesterol