title: Surface-enhanced resonance Raman spectroscopy as an ancillary high-performance liquid chromatography detector for nitrophenol compounds.
abstract: In this study, the potential application of surface-enhanced resonance Raman scattering (SERRS) spectroscopy as an off-line secondary detector for HPLC has been evaluated. Four nitrophenol compounds, 2-nitrophenol, 4-nitrophenol, 2,4-dinitrophenol, and 4,6-dinitrocresol were separated by isocratic reverse-phase high-performance liquid chromatography (RP-HPLC) and monitored with a conventional UV detector. Resonance Raman (RR) and SERRS spectroscopy were next used to provide the required specificity for distinguishing the nitrophenol compounds. The SERRS detection limit for both 2-nitrophenol and 4-nitrophenol was calculated to be 14 ppb and that for 2,4-dinitrophenol and 4,6-dinitrocresol was estimated to lie near the parts-per-billion level as well. This detection limit is 2-3 orders of magnitude lower than that obtained by RR spectroscopy.
authors.forename: F
authors.initials: F
authors.lastname: Ni
authors.forename: L
authors.initials: L
authors.lastname: Thomas
authors.forename: T M
authors.initials: TM
authors.lastname: Cotton
mesh_terms: D002851:Chromatography, High Pressure Liquid; D009596:Nitrophenols / Q000032:analysis*; D013059:Spectrum Analysis, Raman / Q000379:methods
chemical_list: D009596:Nitrophenols