Comparative effects of three beta-lactam antibiotics on anti-PC direct plaque-forming cells.
Penicillin G, cefotaxime and clavulanic acid administered intravenously were studied for their immunomodulating properties. BALBC/c mice were immunized using PC-KLH as thymus-dependent antigen at the same time as the antibiotic was injected. The effect on antibody response was evaluated 5 days after immunization. Critical immunological parameters such as direct antibody-producing cells, Ab-secretion median rate, secretion rate heterogeneity and cellular viability, were studied. The most stimulatory effects were with penicillin G at the 4 x 10(6) IU/kg dose (+73%), cefotaxime at the 366 mg/kg dose (+80%) and clavulanic acid at 1 mg/kg (+218%). The experiments using inhibition of plaque formation with free hapten demonstrate that all the drugs studied decreased the antibody secretion rate and this parameter appeared more heterogeneous when cefotaxime and clavulanic acid were given; however, with penicillin this parameter was more heterogeneous at 2 x 10(5) mg/kg and 1 x 10(3) IU/kg respectively. When clavulanic acid was injected, the number of lymphocytes per spleen, size and friability of the spleen were increased versus the control mice. The present data show that all of the drugs studied present immunostimulating effects on humoral response, against thymus-dependent antigen, but have differently pronounced enhancements.
Corrales
I
I
Department of Microbiology, Faculty of Medicine, Complutense University, Madrid, Spain.
Suarez
A
A
Sanz
R
R
Mato
R
R
Gõmez-Lus
M L
ML
Prieto
J
J
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000921:Antibody-Producing Cells / Q000187:drug effects; D002439:Cefotaxime / Q000494:pharmacology; D002969:Clavulanic Acids / Q000494:pharmacology; D005260:Female; D006462:Hemolytic Plaque Technique; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010400:Penicillin G / Q000494:pharmacology; D010713:Phosphatidylcholines / Q000494:pharmacology; D013154:Spleen / Q000166:cytology
D000900:Anti-Bacterial Agents; D002969:Clavulanic Acids; D010713:Phosphatidylcholines; D002439:Cefotaxime; D010400:Penicillin G