title: [The primary structure of crystallizable monoclonal immunoglobulin IgG1 Kol. II. Amino acid sequence of the L-chain, gamma-type, subgroup I].
abstract: The immunoglobulin Kol was the first intact antibody molecule which was characterized by high-resolution X-ray crystallography. Furthermore the complete amino-acid sequence of the heavy (H)-chain is known. Here we report the complete amino-acid sequence of the light (L)-chain of the monoclonal immunoglobulin Kol (IgG1). The polypeptide has an Mr of 22,781, consists of 216 amino acids and due to its structure is of the lambda-type. With the characteristic amino acids threonine, asparagine, threonine, glycine and lysine in positions 101, 114, 116, 154, and 165, respectively the Kol L-chain is of the Mcg isotype. With the proteins Mcg, Mot, Bur, Loc and Mem six myeloma-derived amino-acid sequences of the same isotype are known. The amino-acid sequence of the N-terminal variable part is characteristic of subgroup 1. This contribution completes the primary structure of IgG1 Kol.
authors.affiliation: max-Planck-Institut für experimentelle Medizin, Abteilung Immunchemie, Göttingen.
authors.forename: H D
authors.initials: HD
authors.lastname: Kratzin
authors.forename: W
authors.initials: W
authors.lastname: Palm
authors.forename: M
authors.initials: M
authors.lastname: Stangel
authors.forename: W E
authors.initials: WE
authors.lastname: Schmidt
authors.forename: J
authors.initials: J
authors.lastname: Friedrich
authors.forename: N
authors.initials: N
authors.lastname: Hilschmann
mesh_terms: D000478:Alkylation; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000911:Antibodies, Monoclonal / Q000032:analysis; D003460:Crystallization; D006868:Hydrolysis; D007074:Immunoglobulin G / Q000032:analysis*; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D014961:X-Ray Diffraction
chemical_list: D000596:Amino Acids; D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G
vernacular_title: Die Primärstruktur des kristallisierbaren monoklonalen Immunglobulins IgG1 Kol. II. Aminosäuresequenz der L-Kette, lambda-Typ, Subgruppe I.