Isotype-specific suppression of IgE synthesis in a human myeloma cell line (U-266) by an IgE-binding protein derived from normal human peripheral blood cells.
Human peripheral blood cells produce immunoglobulin-binding factors upon stimulation with purified protein derivative. The binding factors from 2 healthy individuals were purified by IgE affinity chromatography. IgE-suppressive activity was demonstrable using the IgE-producing human myeloma cell line U-266 as an assay system. The effect of the suppressive factor seems to be reversible, isotype specific, as the immunoglobulin synthesis in the IgGk-producing U-1958 myeloma line was not affected, and selective for the epsilon heavy chain. The results also suggest that this suppression is not the result of a decrease in DNA synthesis or suppression of protein synthesis in general.
Jernberg
H
H
Department of Pathology, University of Uppsala, Sweden.
Nilsson
G
G
Ahlstedt
S
S
Nilsson
K
K
D006801:Humans; D007108:Immune Tolerance; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007132:Immunoglobulin Isotypes / Q000096:biosynthesis; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D008222:Lymphokines / Q000502:physiology*; D029584:Prostatic Secretory Proteins; D013491:Suppressor Factors, Immunologic / Q000502:physiology*; D014407:Tumor Cells, Cultured
D007132:Immunoglobulin Isotypes; D008222:Lymphokines; D029584:Prostatic Secretory Proteins; D013491:Suppressor Factors, Immunologic; C043076:beta-microseminoprotein; C013059:immunoglobulin-binding factors; D007073:Immunoglobulin E