Differentiation of rinderpest and peste des petits ruminants viruses using specific cDNA clones.
The morbilliviruses which infect ruminants, rinderpest (RPV) and peste des petits ruminants (PPRV), are difficult to distinguish serologically. They can be distinguished by differential neutralisation tests and by the migration of the major virus structural protein, the nucleocapsid protein, on polyacrylamide gels. Both these methods are time consuming and require the isolation of live virus for identification; they are not suitable for analysis of material directly from post-mortem specimens. We describe a rapid method for differential diagnosis of infections caused by RPV or PPRV, which uses specific cDNA probes, derived from the mRNAs for the nucleocapsid protein of each virus, which can be used to distinguish unequivocally the two virus types rapidly.
Diallo
A
A
AFRC Institute for Animal Health, Pirbright Laboratory, Surrey, U.K.
Barrett
T
T
Barbron
M
M
Subbarao
S M
SM
Taylor
W P
WP
D000818:Animals; D002213:Capsid / Q000235:genetics*; D003001:Cloning, Molecular; D003429:Cross Reactions; D015342:DNA Probes; D004279:DNA, Viral / Q000235:genetics*; D003937:Diagnosis, Differential; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012367:RNA, Viral / Q000235:genetics; D015183:Restriction Mapping; D012301:Rinderpest / Q000175:diagnosis*; D012302:Rinderpest virus / Q000235:genetics / Q000302:isolation & purification*; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis; D014709:Vero Cells; D014758:Viral Core Proteins / Q000235:genetics*
D015342:DNA Probes; D004279:DNA, Viral; D012367:RNA, Viral; D014758:Viral Core Proteins