Effects of a purified cecropin D from a Chinese silk moth on growth, function and differentiation of murine hemopoietic cells.
The effects of cecropin D, a small basic peptide isolated from a Chinese oak silk moth, on the functions or differentiation of mammalian hemopoietic cells are described in the present paper. This peptide suppressed lectin-induced DNA synthesis of murine splenocytes in a dose-dependent manner without any significant cytotoxic effects. It also exhibited inhibitory effects on antibody production in lipopolysaccharide-stimulated lymphocytes and on colony formation of hemopoietic progenitor cells in plasma clots culture. These results indicate that cecropin D can regulate growth, function and differentiation of murine hemopoietic cells. The biological significance of this finding is discussed from the comparative immunological point of view.
Okai
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Toxicological Research Labs, Kyowa Hakko Kogyo Co., Yamaguchi, Japan.
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D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003114:Colony-Forming Units Assay; D004247:DNA / Q000096:biosynthesis; D006098:Granulocytes / Q000166:cytology / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D066298:In Vitro Techniques; D007301:Insect Hormones / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D019476:Insect Proteins; D007915:Lepidoptera / Q000032:analysis*; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D051379:Mice; D008809:Mice, Inbred C3H; D009036:Moths / Q000032:analysis* / Q000276:immunology
D007301:Insect Hormones; D019476:Insect Proteins; C036149:cecropin D protein, Hyalophora cecropia; D004247:DNA