Double-label and conventional deoxyglucose methods: a practical guide for the user.
The autoradiographic deoxyglucose method is widely used to map functional activity in mammalian brain. Whereas the method is simple to use, the underlying kinetic model is complex. This paper reviews the deoxyglucose kinetic model and the relevant implications for the user who does not have extensive knowledge of tracer kinetics. In generally understandable terms, single-label and double-label deoxyglucose approaches are discussed. Experimental procedures are described in detail. The calculations required for qualitative and quantitative experiments are explained. The deoxyglucose method is compared to other methods that map functional activity in mammalian brain.
Redies
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Department of Brain and Cognitive Sciences, Whitaker College, Massachusetts Institute of Technology, Cambridge 02139.
Gjedde
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D000818:Animals; D001345:Autoradiography / Q000379:methods*; D001921:Brain / Q000378:metabolism*; D003837:Deoxy Sugars; D003847:Deoxyglucose
D003837:Deoxy Sugars; D003847:Deoxyglucose