title: ELISA for human pancreatic elastase 1.
abstract: A sandwich-type ELISA for human pancreatic elastase 1 was developed and assessed for clinical feasibility. Using highly purified reagents, the test seems to be as sensitive and specific as the well-documented Radio-immunoassay (RIA) for the detection of elevated elastase 1 serum levels and, consequently, would have the same indications described for the RIA. In addition, the ELISA could be valid as a screening test for cystic fibrosis. Because ELISA reagents are relatively stable, the assay is always ready to use and can be performed without special equipment.
authors.affiliation: Institute of Medical Microbiology, University of Giessen, FRG.
authors.forename: A
authors.initials: A
authors.lastname: Sziegoleit
authors.forename: H
authors.initials: H
authors.lastname: Knäpler
authors.forename: B
authors.initials: B
authors.lastname: Peters
mesh_terms: D004796:Clinical Enzyme Tests; D003550:Cystic Fibrosis / Q000175:diagnosis; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D010179:Pancreas / Q000201:enzymology*; D010196:Pancreatic Elastase / Q000032:analysis*; D011863:Radioimmunoassay
chemical_list: D010196:Pancreatic Elastase