title: Calbindin-D 28K in hippocampal organotypic cultures.
abstract: Slices of hippocampus from 6-day-old rats were cultured for 2-4 weeks using the roller-tube technique. The organization of these explants was studied by immunocytochemical labeling of calbindin-D 28K (CaBP 28K). The development of the CaBP 28K staining was very close to that of the rat hippocampus in vivo with only 3 subpopulations of labeled cells: granule cells and their mossy fibers, pyramidal cells in the subiculum-CA1 zone and interneurons scattered in strata oriens and radiatum.
authors.affiliation: I.N.S.E.R.M. U. 29, Hôpital de Port-Royal, Paris, France.
authors.forename: N
authors.initials: N
authors.lastname: Bousez-Dumesnil
authors.forename: M
authors.initials: M
authors.lastname: Thomasset
authors.forename: Y
authors.initials: Y
authors.lastname: Ben-Ari
mesh_terms: D000818:Animals; D064026:Calbindins; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D009924:Organ Culture Techniques; D051381:Rats; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*
chemical_list: D064026:Calbindins; D064030:S100 Calcium Binding Protein G