title: Ki-67 labeling index in breast cancer.
abstract: Measurements of cell cycle kinetics have been found to correlate with the clinical course of patients with breast cancer. However, the thymidine labeling index and more rapid methods like flow cytometry remain complicated and costly. We assessed cell proliferation of 67 breast carcinomas by an immunoperoxidase procedure using a monoclonal antibody, Ki-67, which reacts with a nuclear antigen in proliferating cells. The percentage of Ki-67 positive cells ranged from 2% to 70%. Tumors with high mitotic rate, high nuclear grade, high histologic grade, and negative estrogen receptors had statistically higher Ki-67 labeling rates. We found no significant differences between the Ki-67 labeling rate and other clinical (age at diagnosis, menopausal status) or pathologic (necrosis, fibrosis, vascular invasion, lymphatic invasion, cellular reaction, tumor size, lymph node metastases) features assessed. These results parallel previously reported data, and confirm that this immunohistochemical staining of breast carcinoma by Ki-67 monoclonal antibody can be considered a rapid and convenient method for assessing cell cycle kinetics. However, further studies, evaluating the correlation between Ki-67 labeling rate and prognosis are needed to better define the real usefulness of this analysis in clinical practice.
authors.affiliation: Divisione di Radioterapia Oncologica, Nuovo Ospedale San Gerardo Monza, Milano-Italy.
authors.forename: S
authors.initials: S
authors.lastname: Crispino
authors.forename: A
authors.initials: A
authors.lastname: Brenna
authors.forename: D
authors.initials: D
authors.lastname: Colombo
authors.forename: B
authors.initials: B
authors.lastname: Flores
authors.forename: S
authors.initials: S
authors.lastname: D'Amico
authors.forename: P
authors.initials: P
authors.lastname: Lissoni
authors.forename: S
authors.initials: S
authors.lastname: Barni
authors.forename: F
authors.initials: F
authors.lastname: Paolorossi
authors.forename: G
authors.initials: G
authors.lastname: Bratina
authors.forename: G
authors.initials: G
authors.lastname: Tancini
mesh_terms: D000911:Antibodies, Monoclonal; D001943:Breast Neoplasms / Q000473:pathology*; D002277:Carcinoma / Q000473:pathology* / Q000556:secondary; D002453:Cell Cycle; D005260:Female; D005355:Fibrosis; D006801:Humans; D007124:Immunoenzyme Techniques; D008207:Lymphatic Metastasis; D008875:Middle Aged; D008940:Mitotic Index; D009336:Necrosis; D009367:Neoplasm Staging; D011960:Receptors, Estrogen / Q000032:analysis
chemical_list: D000911:Antibodies, Monoclonal; D011960:Receptors, Estrogen