Balloon catheter injury to rabbit carotid artery. II. Selective increase in reactivity to some vasoconstrictor drugs.
The present study examined the changes in reactivity to a variety of vasoconstrictor drugs of the rabbit carotid artery during development of an intimal thickening induced by injury with an inflated balloon catheter. The injured and the unoperated contralateral carotid arteries were studied at 2 and 6 weeks after the operation. To differentiate areas of the injured artery lined by modified smooth muscle cells from areas lined by regenerated endothelial cells, each rabbit was injected with Evans blue dye before sacrifice. Ring segments (3 mm length) from the control and injured arteries were mounted in organ baths to record the circumferential isometric force with a technique that ensured that all rings were set to equivalent initial resting conditions of passive transmural stretch. Compared with the controls, the experimental arteries had a significantly decreased maximum contraction (Emax) in response to KCl at both 2 and 6 weeks. The experimental arteries were also significantly less sensitive to the alpha 1-adrenoceptor agonist, methoxamine, at both 2 weeks (approximately sevenfold) and 6 weeks (fourfold), with a marked decrease in Emax at 2 weeks, which returned to control values at 6 weeks. There was no change in Emax to either serotonin or the thromboxane A2-mimetic, U46619, in the experimental arteries at either time. There was, however, a small but significant increase in the sensitivity to both drugs. There was no difference in response to any of the constrictor agents between the white and blue regions of the experimental vessels.(ABSTRACT TRUNCATED AT 250 WORDS)
Manderson
J A
JA
Department of Anatomy, University of Melbourne, Parkville, Victoria, Australia.
Cocks
T M
TM
Campbell
G R
GR
D019796:15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid; D000818:Animals; D002339:Carotid Arteries / Q000187:drug effects* / Q000503:physiopathology; D020212:Carotid Artery Injuries; D002404:Catheterization / Q000009:adverse effects*; D004730:Endothelium, Vascular / Q000473:pathology; D066298:In Vitro Techniques; D008297:Male; D008729:Methoxamine / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D011450:Prostaglandin Endoperoxides, Synthetic / Q000494:pharmacology; D011817:Rabbits; D012701:Serotonin / Q000494:pharmacology; D014661:Vasoconstriction / Q000187:drug effects; D014662:Vasoconstrictor Agents / Q000494:pharmacology*
D011450:Prostaglandin Endoperoxides, Synthetic; D014662:Vasoconstrictor Agents; D012701:Serotonin; D011189:Potassium Chloride; D019796:15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid; D008729:Methoxamine