Somatic cell-oocyte interactions in mouse oogenesis: stage-specific regulation of mouse oocyte protein phosphorylation by granulosa cells.
The relative rate of synthesis of a number of proteins and the protein phosphorylation pattern of growing and fully grown oocytes were influenced by the presence of granulosa cells. In particular, a 74-kDa phosphorylated protein was detected only in granulosa cell-enclosed growing mouse oocytes. When reaggregated with granulosa cells, the growing oocyte displayed the phosphorylated form of the 74-kDa protein but when oocytes were cultured on Sertoli cell monolayers or in granulosa cell-conditioned medium the 74-kDa protein was not phosphorylated. We propose that (1) granulosa cells regulate protein phosphorylation in mouse oocytes; (2) a 74-kDa protein is phosphorylated only in growing oocytes when surrounded by granulosa cells; and (3) granulosa cells, but not Sertoli cells, are competent to send the appropriate "signal" to the growing oocyte.
Colonna
R
R
Dipartimento di Scienze e Tecnologie Biomediche e di Biometria, Universita' dell'Aquila, Italy.
Cecconi
S
S
Tatone
C
C
Mangia
F
F
Buccione
R
R
D000818:Animals; D002450:Cell Communication; D002478:Cells, Cultured; D005260:Female; D006107:Granulosa Cells / Q000502:physiology*; D008297:Male; D051379:Mice; D008970:Molecular Weight; D009865:Oocytes / Q000502:physiology*; D009866:Oogenesis; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D012708:Sertoli Cells / Q000502:physiology
D010750:Phosphoproteins