A basic phospholipase A from the venom of the Australian king brown snake (Pseudechis australis) showing diverse activities against membranes.
1. A basic phospholipase A (MSPA) was isolated from the venom of the Australian king brown snake, Pseudechis australis. 2. MSPA had an approximate Mr of 13,000 and consisted of a single polypeptide chain of 119 amino acid residues cross-linked by seven disulphide bridges. 3. MSPA exhibited direct haemolytic, anticoagulant and myotoxic activities. 4. Treatment of MSPA with p-bromophenacyl bromide modified a single histidine residue, resulting in complete loss of enzyme activity.
Sharp
P J
PJ
School of Chemistry, Macquarie University, NSW, Australia.
Berry
S L
SL
Spence
I
I
Howden
M E
ME
D000098:Acetophenones / Q000494:pharmacology; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000925:Anticoagulants / Q000302:isolation & purification; D004546:Elapid Venoms / Q000032:analysis*; D006461:Hemolysis / Q000187:drug effects; D007526:Isoelectric Point; D008566:Membranes / Q000187:drug effects; D008970:Molecular Weight; D009132:Muscles / Q000187:drug effects; D010740:Phospholipases / Q000302:isolation & purification*; D010741:Phospholipases A / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000494:pharmacology
D000098:Acetophenones; D000596:Amino Acids; D000925:Anticoagulants; D004546:Elapid Venoms; D010740:Phospholipases; D010741:Phospholipases A; C006821:4-bromophenacyl bromide