Tissue specificity in the expression of Friend erythroleukemic virus sequences in infected mouse tissues.
The SQA cell line produces Friend leukemia virus that remains leukemogenic after serial passages in vitro. The state of the provirus and its expression were investigated in newborn and adult mouse tissues, using probes specific for ecotropic and xenotropic sequences. Genomic ecotropic and xenotropic sequences were similar in size in spleen and liver of infected and control animals but appear amplified in infected tissues. Expression of these sequences however differed. Several species of xenotropic and ecotropic-specific RNAs were detected in infected spleens, in SQA cells and in the liver of newborn infected animals but were absent in infected adult liver and control tissues. These results suggest that activation and expression of ecotropic and xenotropic endogenous sequences may play a role in pathogenesis of the disease.
Joesten
M E
ME
Center for Experimental Cell Biology, Mount Sinai School of Medicine, City University of New York, NY 10029.
Royston
M E
ME
Pogo
B G
BG
D000367:Age Factors; D000818:Animals; D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005622:Friend murine leukemia virus / Q000235:genetics*; D015151:Immunoblotting; D066298:In Vitro Techniques; D007942:Leukemia, Experimental / Q000382:microbiology*; D051379:Mice; D009928:Organ Specificity; D011533:Proviruses / Q000235:genetics*; D012367:RNA, Viral / Q000032:analysis; D013154:Spleen / Q000382:microbiology
D004279:DNA, Viral; D012367:RNA, Viral