Use of the glucose oxidase/peroxidase method for glucose assay leads to overestimation of the inhibition of gluconeogenesis by aminopyrine.
4-Aminoantipyrine strongly inhibits glucose determination by the glucose oxidase/peroxidase/dianisidine assay but does not interfere in the assay using glucose-6-phosphate dehydrogenase, hexokinase and ATP. As a result, the inhibition of gluconeogenesis by aminopyrine reported to be 50-90% (Bánhegyi, G., Mandl, J., Antoni, F. and Garzó, T. (1987) Biochim. Biophys. Acta 927, 406-416) is strongly overestimated and amounts to only 10-30%.
Latos
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Department of Cellular Biochemistry, Nencki Institute of Experimental Biology, Warsaw, Poland.
Wojtczak
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D000632:Aminopyrine / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D005189:False Positive Reactions; D005943:Gluconeogenesis / Q000187:drug effects*; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase; D008099:Liver / Q000378:metabolism; D008722:Methods; D010544:Peroxidases; D051381:Rats
D000632:Aminopyrine; D005949:Glucose Oxidase; D010544:Peroxidases; C023057:glucose peroxidase; D005947:Glucose