Identity of acyl-CoA oxidase with glutaryl-CoA oxidase.
Rats fed on clofibrate- and DEHP-containing diets showed virtually proportional increases in hepatic acyl-CoA oxidase and glutaryl-CoA oxidase activities. The solubilization profiles of the two activities from the light mitochondrial fraction of the liver homogenate of DEHP-treated rats were the same, and the glutaryl-CoA oxidase/acyl-CoA oxidase activity ratio remained constant through the purification. The final preparation obtained was a single protein based on the result of polyacrylamide gel electrophoresis. The evidence indicates that the two activities are associated with the same protein.
Horie
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Department of Clinical Biochemistry, Tokyo College of Pharmacy, Japan.
Ogawa
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Suga
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D042962:Acyl-CoA Oxidase; D000818:Animals; D002994:Clofibrate / Q000494:pharmacology; D004051:Diethylhexyl Phthalate / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000201:enzymology; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology; D010088:Oxidoreductases / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012995:Solubility; D013431:Sulfates / Q000494:pharmacology; D015032:Zinc / Q000494:pharmacology; D019287:Zinc Sulfate
D013431:Sulfates; D019287:Zinc Sulfate; D004051:Diethylhexyl Phthalate; D010088:Oxidoreductases; C042428:glutaryl coenzyme A oxidase; D042962:Acyl-CoA Oxidase; D002994:Clofibrate; D015032:Zinc