title: Techniques of sperm selection, improvement and separation in an in vitro fertilization program.
abstract: The Authors present a review of the various techniques used for improving the fertility potential of sperm within the context of an in vitro fertilization (IVF) program. After a brief description of the mechanisms leading to normal in vivo fertilization, they discuss the different methods of selecting and improving sperm for IVF. They conclude that centrifugation on discontinuous Percoll gradients would seem to be the most efficient separation method from all points of view, while the addition of pharmacological agents to improve sperm quality and motility lead to extremely unsatisfactory results.
authors.affiliation: Openbaar Centrum voor Maatschappelijk Welzijn Genees- en Heelkundig Instituut, Vilvoorde.
authors.forename: P
authors.initials: P
authors.lastname: Van der Zwalmen
authors.forename: G
authors.initials: G
authors.lastname: Bertin
authors.forename: A
authors.initials: A
authors.lastname: Schoysman-Deboeck
authors.forename: L
authors.initials: L
authors.lastname: Geerts
authors.forename: R
authors.initials: R
authors.lastname: Schoysman
authors.forename: C
authors.initials: C
authors.lastname: Debauche
mesh_terms: D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D003470:Culture Media; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D013048:Specimen Handling; D013075:Sperm Capacitation; D013081:Sperm Motility; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000002:abnormalities / Q000187:drug effects
chemical_list: D003470:Culture Media