[Determination of isepamicin in the eluates from the dried blood spots on filter paper for monitoring of blood levels in a guinea-pig].
In general, collection of serial blood samples from small experimental animals is difficult in terms of sampling site and operation technique. To overcome these problems, a simple reproducible method has been improved by the use of filter papers. Whole blood obtained by venipuncture from the ear vein of guinea-pig was spotted onto a filter paper. Isepamicin in the dried blood spot was extracted with 0.5 M Na2HPO4 buffer by incubation and determined by fluorescence polarization immunoassay. Linearity was established over the range of 5-150 micrograms/ml by using only 100 microliters of whole blood. Consequently its accuracy and precision were good, with mean coefficient of variation of less than 5%. The method described here correlates well with a conventional sampling method and could be used for the pre-clinical study of isepamicin blood levels of individual guinea-pigs. This method is suitable for the simultaneous measurement of aminoglycoside antibiotics or physiological parameters after the administration of aminoglycoside antibiotics for the pharmacokinetics study.
Kase
K
K
Shoshihara
M
M
Tsuda
Y
Y
Yoshizawa
E
E
Fujimoto
T
T
D000818:Animals; D001800:Blood Specimen Collection / Q000379:methods*; D005455:Fluorescent Antibody Technique; D005839:Gentamicins / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D006168:Guinea Pigs; D007273:Injections, Intramuscular; D008297:Male; D010209:Paper
D005839:Gentamicins; C016927:isepamicin