title: Rabbit placental-conditioned medium stimulates progesterone accumulation by granulosa-lutein cells in culture: preliminary characterization of a placental luteotropic hormone.
abstract: The rabbit fetal placenta plays an important physiological role in luteal maintenance in pregnancy, probably via the secretion of an unidentified placental "luteotropin." The objective of these studies was to examine conditioned medium from fetal placental-tissue incubations (FPI) for the presence of placental luteotropic hormones/factors, using the stimulation of progesterone accumulation by rabbit granulosa-lutein cells in culture, as an in vitro luteotropic bioassay. Progesterone accumulation by rabbit granulosa-lutein cells (during 5 days of culture) was increased (compared with controls), 1.5-fold by 10(-8) M estradiol-17 beta (E2) and 11.5-fold by 100 ng/ml luteinizing hormone (oLH). FPI stimulated progesterone accumulation (approximately 3-fold) and this was further increased in the presence of E2 (FPI + E2; approximately 6-fold). Luteotropic bioactivity in FPI (+ E2) was retained after dialysis (6000-8000 MW cutoff; 7.8-fold) and heating (90-95 degrees C for 1 h; 7.5-fold), but was destroyed after incubation with trypsin (1 mg/ml, 1 h at 37 degrees C; 0.9-fold). Media conditioned with skeletal muscle (1.2-fold), heart (1.6-fold), liver (1.5-fold), and uterus (0.5-fold) and 5-10% serum (less than 1-fold), from pseudopregnant rabbits, had little or no luteotropic bioactivity. These data indicate that FPI contains a luteotropic hormone/factor that is probably a heat-stable, trypsin-sensitive, protein/peptide of greater than 6000-8000 MW that acts in synergy with E2 to promote granulosa-lutein cell steroidogenesis. This placental hormone/factor is a good candidate for the elusive rabbit placental luteotropin.
authors.affiliation: Department of Anatomy, Physiological Sciences and Radiology, College of Veterinary Medicine, North Carolina State University, Raleigh 27606.
authors.forename: J E
authors.initials: JE
authors.lastname: Gadsby
authors.forename: M E
authors.initials: ME
authors.lastname: Lancaster
mesh_terms: D000818:Animals; D002452:Cell Count; D002478:Cells, Cultured; D003338:Corpus Luteum / Q000378:metabolism*; D003470:Culture Media / Q000032:analysis / Q000494:pharmacology; D003956:Dialysis; D004958:Estradiol / Q000378:metabolism; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism*; D006358:Hot Temperature; D066298:In Vitro Techniques; D008184:Luteal Cells / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000378:metabolism; D010920:Placenta / Q000378:metabolism*; D010926:Placental Hormones / Q000302:isolation & purification / Q000378:metabolism*; D011247:Pregnancy; D011374:Progesterone / Q000378:metabolism*; D011817:Rabbits; D011863:Radioimmunoassay; D014357:Trypsin
chemical_list: D003470:Culture Media; D010926:Placental Hormones; D011374:Progesterone; D004958:Estradiol; D007986:Luteinizing Hormone; D014357:Trypsin