Anti-filarial effects of nine quinoline-containing drugs on adult filariae in vitro.
The potencies and efficacies of 9 quinoline-containing anti-malarials including chloroquine, (bis)desethylchloroquine, SN6911, SN12108, amodiaquine, CN-2999-2K, primaquine, quinacrine, and quinine were examined in vitro against adult female Brugia pahangi. Parasite motility and lactate excretion were measured as indicators of drug effects. All of the agents tested showed time-dependent increases in potency over a 24-72-hr incubation period. SN12108 was the most potent at 72 hr, reducing motility by greater than or equal to 50% (IC50) at 1.0 x 10(-7) M. Chloroquine (IC50 2.3 x 10(-6) M), desethylchloroquine (IC50 7.0 x 10(-6) M), quinacrine (IC50 1.9 x 10(-6) M), and quinine (IC50 1.5 x 10(-5) M) were the least potent. All of the drugs caused time-dependent decreases in lactate excretion, except quinine; decreases were found to be dose dependent. A high correlation (r greater than 0.85) was seen between time-dependent effects on motility and lactate excretion. The effects of chloroquine (10 microM) on motility were also examined in female Acanthocheilonema viteae, Dirofilaria immitis, Onchocerca volvulus, and male Onchocerca gutturosa. Dirofilaria immitis was less sensitive to chloroquine than B. pahangi; A. viteae was equally sensitive. Species of Onchocerca were the most sensitive parasites studied. Adult O. gutturosa and O. volvulus were affected by 10 microM chloroquine within 4-6 hr; motility was reduced by 80% within 24 hr. Although the mechanism of anti-filarial activity of the quinoline-containing drugs is not known, their in vitro activity against a variety of adult filariae at clinically relevant concentrations, as well as differential sensitivity seen between the different filariae examined, warrants further study of these compounds.
VandeWaa
E A
EA
Department of Microbiology and Public Health, Michigan State University, East Lansing 48824.
Bennett
J L
JL
Williams
J F
JF
Satti
M Z
MZ
Geary
T G
TG
D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D000962:Antimalarials / Q000494:pharmacology*; D002009:Brugia / Q000187:drug effects*; D002738:Chloroquine / Q000494:pharmacology; D004153:Dipetalonema / Q000187:drug effects; D004183:Dirofilaria immitis / Q000187:drug effects; D005260:Female; D005369:Filaricides / Q000494:pharmacology*; D005370:Filarioidea / Q000187:drug effects*; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008297:Male; D009068:Movement / Q000187:drug effects; D009854:Onchocerca / Q000187:drug effects; D011804:Quinolines / Q000494:pharmacology*; D013997:Time Factors
D000871:Anthelmintics; D000962:Antimalarials; D005369:Filaricides; D007773:Lactates; D011804:Quinolines; D019344:Lactic Acid; D002738:Chloroquine