title: Liquid chromatographic method for quantitation of glyphosate and metabolite residues in organic and mineral soils, stream sediments, and hardwood foliage.
abstract: A liquid chromatographic method for determining glyphosate (GLYPH) and its major metabolite aminomethylphosphonic acid (AMPA) in various environmental substrates is described. Ion-exchange column chromatography is coupled with post-column ninhydrin derivatization and absorbance detection at 570 nm. Use of a valve-switching technique allowed quantitation of both analytes in a single chromatographic run and eliminated slow-eluting, coextracted interferences. The method was successfully used to quantitate GLYPH and AMPA in organic and mineral soils, stream sediments, and foliage of 2 hardwood brush species. Mean recovery efficiencies for GLYPH as determined from fortified blank field samples were as follows: bottom sediment 84%, suspended sediment 66%, organic soils 79%, mineral soils 73%, alder leaf litter 81%, salmonberry leaf litter 84%, and artificial deposit collectors 87%. Precision for GLYPH determination was good with less than 14% coefficient of variation on mean recovery for all substrates. Limits of detection were lowest for sediments (0.01 microgram/g dry mass) and highest for foliage substrates (0.10 microgram/g dry mass). Using this system, 6 samples/person/day were routinely analyzed.
authors.affiliation: Canadian Forestry Service, Forest Pest Management Institute, Marie, Ontario.
authors.forename: D G
authors.initials: DG
authors.lastname: Thompson
authors.forename: J E
authors.initials: JE
authors.lastname: Cowell
authors.forename: R J
authors.initials: RJ
authors.lastname: Daniels
authors.forename: B
authors.initials: B
authors.lastname: Staznik
authors.forename: L M
authors.initials: LM
authors.lastname: MacDonald
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005998:Glycine / Q000031:analogs & derivatives* / Q000032:analysis; D012989:Soil Pollutants / Q000032:analysis*; D014475:Uncoupling Agents / Q000032:analysis*; D014873:Water Pollutants / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis*; D014934:Wood; D000097797:Glyphosate
chemical_list: D005998:Glycine; D012989:Soil Pollutants; D014475:Uncoupling Agents; D014873:Water Pollutants; D014874:Water Pollutants, Chemical; D000097797:Glyphosate