Differential expression of cell adhesion molecules in variants of K1735 melanoma cells differing in metastatic capacity.
We have investigated the expression of 2 neural-cell adhesion molecules, NCAM and LI, in K1735-C116 and -MI melanoma cells which differ qualitatively in their metastatic potential, i.e., MI cells are metastatic whereas C116 cells are not. We have found that NCAM in C116 cells are expressed as 2 quantitatively major glycosylated polypeptides with Mr of 145,000 and 120,000 and a minor 190,000 Mr polypeptide, whereas MI cells expressed NCAM as 3 glycosylated polypeptides with MR of 200,000, 140,000 and 120,000. The amount of NCAM in MI cells constituted only 60% of the amount observed in C116 cells. In C116 cells, the 145,000 and 120,000 Mr NCAM polypeptides were sulphated whereas NCAM did not appear to be sulphated in MI cells. No phosphorylation of NCAM in the 2 cell lines was observed. LI was expressed as a phosphorylated glycoprotein with Mr of 210,000 in MI cells whereas no LI expression was observed in C116 cells. LI was not sulphated in MI cells.
Linnemann
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Research Center for Medical Biotechnology, University of Copenhagen, Denmark.
Raz
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Bock
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D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000302:isolation & purification; D000954:Antigens, Surface / Q000032:analysis* / Q000302:isolation & purification; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules; D002460:Cell Line; D006031:Glycosylation; D008175:Lung Neoplasms / Q000276:immunology*; D008546:Melanoma, Experimental / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D008970:Molecular Weight; D009362:Neoplasm Metastasis; D010766:Phosphorylation; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D015815:Cell Adhesion Molecules