Purkinje cell axon collateral distributions reflect the chemical compartmentation of the rat cerebellar cortex.
Monoclonal antibody mabQ113 has been used to study the distribution of Purkinje cell axon collaterals in the rat cerebellar cortex. MabQ113 recognizes a polypeptide antigen, zebrin I, that is confined to a subset of Purkinje cells. Antigenic Purkinje cells are arranged in parasagittal compartments running throughout the cortex. No other cerebellar cells are immunoreactive. Immunoreactive axon collaterals are confined principally to the infraganglionic plexus with only a few extending into the molecular layer. Three probable target cells for the axon collaterals have been identified: Golgi cells, Lugaro cells, and other Purkinje cells. About 90% of immunoreactive axon collaterals in the anterior lobe are located beneath the mabQ113+ Purkinje cell compartment in which they originate but some do invade the neighboring mabQ113- territory. In the anterior lobe vermis, the distribution of invading mabQ113+ collaterals is not symmetrical, such that the probability of an invading collateral from the P2+ compartment is greater at the medial boundary into P1- than at the lateral into P2-. The distribution of immunoreactive collaterals is consistent with their playing a role in synchronizing the firing of Purkinje cells within the same compartment.
Hawkes
R
R
Laboratory of Neurobiology, Faculty of Medicine, Laval University, Quebec, Canada.
Leclerc
N
N
D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000378:metabolism*; D001369:Axons / Q000378:metabolism*; D002525:Cerebellar Cortex / Q000166:cytology / Q000378:metabolism*; D005260:Female; D007150:Immunohistochemistry; D051379:Mice; D011689:Purkinje Cells / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D000911:Antibodies, Monoclonal; D000941:Antigens