Participation of mannose receptors on the surface of stimulated macrophages in the phagocytosis of glutaraldehyde-fixed Candida albicans, in vitro.
We have evaluated the participation of mannose receptors on the surface of stimulated macrophages in the phagocytosis of Candida albicans in vitro. A dose-dependent 8.6 to 88.3% reduction of phagocytosis was observed in the presence of 0.5 to 5.0 mg/ml of the mannose-rich glycoprotein invertase (either native or denatured) in the incubation medium. Macrophages plated onto substrates coated with poly-L-lysine-mannan also showed a 99% reduction of phagocytic activity toward Candida albicans, but phagocytosis of IgG-coated erythrocytes was not inhibited under the same conditions. These results indicate that mannose receptors are involved in one of the initial steps of phagocytosis of Candida albicans by macrophages.
Felipe
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I
Departamento de Patologia Geral, Universidade Estadual de Londrina, PR, Brasil.
Bim
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S
Loyola
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D000818:Animals; D002176:Candida albicans; D002462:Cell Membrane / Q000378:metabolism; D003470:Culture Media; D066298:In Vitro Techniques; D037181:Lectins, C-Type; D008264:Macrophages / Q000502:physiology*; D008351:Mannans / Q000378:metabolism; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D051379:Mice; D010587:Phagocytosis; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic / Q000502:physiology*
D003470:Culture Media; D037181:Lectins, C-Type; D008351:Mannans; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic