Chromosome aberrations in spermatogonia and sperm abnormalities in Curacron-treated mice.
Curacron is an organophosphorus pesticide widely used in cotton fields. In order to assay its mutagenic potential in mammalian germ cells chromosomal aberrations in spermatogonial cells and sperm abnormalities were examined in mice after Curacron treatment. For studying chromosomal aberrations mice were treated both acutely (single treatment) and subacutely (for 5 consecutive days) with 3 dose levels of Curacron, 12, 36 and 72 mg/kg. Curacron was found to produce a significant increase in structural chromosomal aberrations after acute and subacute treatments. This increase was dose-dependent. A dose-dependent inhibition in mitotic activity in spermatogonia was also found. For studying sperm abnormalities mice were treated for 5 consecutive days with 20, 40 and 60 mg/kg. Morphological sperm abnormalities increased significantly after treatment with Curacron. The increase was dose-dependent. An inhibition of 40.2% in sperm count and of 74.5% in sperm motility occurred after treatment with 60 mg/kg Curacron. These results show that Curacron has a damaging effect on spermatogonial cells as well as on sperm morphology.
el Nahas
S M
SM
Unit of Cell Biology, National Research Centre, Cairo, Egypt.
de Hondt
H A
HA
Abdou
H E
HE
D000818:Animals; D002869:Chromosome Aberrations; D004305:Dose-Response Relationship, Drug; D008297:Male; D051379:Mice; D008938:Mitosis / Q000187:drug effects; D009929:Organ Size / Q000187:drug effects; D063086:Organothiophosphates / Q000633:toxicity*; D009946:Organothiophosphorus Compounds / Q000633:toxicity*; D013076:Sperm Count / Q000187:drug effects; D013081:Sperm Motility / Q000187:drug effects; D013091:Spermatogenesis / Q000187:drug effects*; D013093:Spermatogonia / Q000187:drug effects*; D013094:Spermatozoa / Q000002:abnormalities* / Q000187:drug effects*; D013737:Testis / Q000033:anatomy & histology
D063086:Organothiophosphates; D009946:Organothiophosphorus Compounds; C024273:profenofos