Effects of pertussis toxin and indomethacin on murine lymphocytes in the bronchoalveolar lavage fluids.
The total number of lymphocytes in the bronchoalveolar lavage (BAL) fluids significantly increased in mice injected intravenously with pertussis toxin (PT), while the absolute number of alveolar macrophages markedly decreased. This finding probably reflects the lymphocyte accumulation in interstitial spaces as we previously observed in mice injected with PT. In addition, indomethacin, at lower dosage (0.5 mg/kg) prevented peripheral lymphocytosis and lymphocyte accumulation in the alveolar spaces of the lungs of mice injected with PT. These results provide evidence that PT is responsible for lymphocyte accumulation together with a marked decrease of alveolar macrophages in the lungs of treated mice; moreover, indomethacin is effective in preventing bronchoalveolar changes caused by PT.
Torre
D
D
Division of Infectious Diseases, Regional Hospital and Foundation E. and S. Macchi, Varese, Italy.
Pugliese
A
A
Quadrelli
C
C
Sampietro
C
C
Rossi
S
S
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D002452:Cell Count; D005260:Female; D007213:Indomethacin / Q000494:pharmacology*; D007958:Leukocyte Count; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D037342:Pertussis Toxin; D010566:Virulence Factors, Bordetella / Q000037:antagonists & inhibitors / Q000633:toxicity*
D010566:Virulence Factors, Bordetella; D037342:Pertussis Toxin; D007213:Indomethacin