[Rapid typing of Escherichia coli K antigens using bacteriophages].
E. coli capsular (K) antigens are important virulence factors contributing to the development of urinary tract infections (UTI). Serotyping of these antigens is laborious and depends on the availability of respective antisera which are difficult to prepare because of the low immunogenicity of these polysaccharide antigens. The application of specific K phages are a big advantage. Two collections of E. coli strains (500 from Rostock, 214 from Erfurt) isolated from patients with UTI have been investigated with a set of ten K specific bacteriophages including phi 1, phi 2, phi 5, phi 7, phi 9, phi 12, phi 13, phi 20, phi 30 and phi 36. The K1 antigen has been found to be the most frequent capsular antigen (16.4% in Rostock; 12.2% in Erfurt) followed by K13/K20 in Erfurt (8.9%) and K5 in Rostock (8%) and Erfurt (7.5%). The collection of phages proved to be suitable for the detection of UTI associated K antigens covering the most common O serogroups found in UTI. The method appears to be simple, non-laborious and applicable for routine use.
Nimmich
W
W
Zingler
G
G
Falkenhagen
U
U
Naumann
G
G
Schulz
S
S
Wachtel
D
D
D000942:Antigens, Bacterial; D000954:Antigens, Surface / Q000302:isolation & purification*; D001434:Bacteriophage Typing / Q000379:methods*; D001437:Bacteriuria / Q000382:microbiology*; D004926:Escherichia coli / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology*; D006801:Humans; D014774:Virulence
D000942:Antigens, Bacterial; D000954:Antigens, Surface; C030659:K antigens
Schnelltypisierung von Escherichia coli-K-Antigenen mit Hilfe von Bakteriophagen.