title: In vivo action of alpha-amylase inhibitor from cranberry bean (Phaseolus vulgaris) in rat small intestine.
abstract: An alpha-amylase inhibitor prepared from cranberry bean (Phaseolus vulgaris) was examined for its in vivo action on pancreatic alpha-amylase in rat small intestine. For this purpose, postprandial changes not only in intraluminal alpha-amylase activity but also in plasma glucose and insulin concentrations were measured at various times after administration of 2 g of 10% polyethylene glycol-containing experimental diets with and without the inhibitor. No considerable increase was observed in the levels of intraluminal alpha-amylase activity, blood sugar, and plasma insulin in the animals given the inhibitor at a dose of 10 mg each. These results suggest that the purified preparation from cranberry bean serves in fact as a potent inhibitor of rat pancreatic alpha-amylase.
authors.affiliation: Department of Home Economics, Koka Women's Junior College, Kyoto, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kotaru
authors.forename: K
authors.initials: K
authors.lastname: Iwami
authors.forename: H Y
authors.initials: HY
authors.lastname: Yeh
authors.forename: F
authors.initials: F
authors.lastname: Ibuki
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D007887:Fabaceae / Q000032:analysis*; D005502:Food; D005746:Gastric Emptying / Q000187:drug effects; D007328:Insulin / Q000097:blood; D007421:Intestine, Small / Q000201:enzymology*; D008297:Male; D010179:Pancreas / Q000201:enzymology; D010946:Plants, Medicinal; D051381:Rats; D011919:Rats, Inbred Strains; D000516:alpha-Amylases / Q000037:antagonists & inhibitors*
chemical_list: D001786:Blood Glucose; D007328:Insulin; D000516:alpha-Amylases