Sister chromatid exchanges induced by tertiary butyl hydroquinone in bone marrow cells of mice.
Tertiary butyl hydroquinone (TBHQ)--a phenolic antioxidant, was evaluated by assessing the induction of sister chromatid exchanges (SCEs) in bone marrow metaphase cells of mice. Six concentrations, 0.5, 2, 20, 50, 100, and 200 mg/kg, of TBHQ were injected intraperitoneally. A positive dose-response effect in the SCE frequency was observed using the Cochran-Armitage trend test. Two mg/kg of TBHQ was found to be the minimum effective dose. Study of the cell-cycle kinetics showed a delay in cell cycle induced by the higher concentrations of TBHQ. Thus, TBHQ was found to be a DNA damage-inducing agent and also a cytotoxic chemical in vivo in mice.
Mukherjee
A
A
Department of Botany, University of Calcutta, India.
Talukder
G
G
Sharma
A
A
D000818:Animals; D001854:Bone Marrow Cells; D002453:Cell Cycle / Q000187:drug effects; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D006873:Hydroquinones / Q000633:toxicity*; D051379:Mice; D012854:Sister Chromatid Exchange
D006873:Hydroquinones; C018855:2-tert-butylhydroquinone