A method for direct in vivo measurement of drug concentrations from a single 2H NMR spectrum.
The use of 2H-labeled drugs provides a measure of drug concentration in situ directly from a single 2H NMR spectrum obtained with any antenna by correcting only for differential saturation effects. The limit of detection for a drug labeled with three equivalent deuterons is roughly 0.5 mM.
Evelhoch
J L
JL
Department of Internal Medicine, Wayne State University School of Medicine, Detroit, Michigan 48201.
McCoy
C L
CL
Giri
B P
BP
D000818:Animals; D003903:Deuterium; D005069:Evaluation Studies as Topic; D005260:Female; D005354:Fibrosarcoma / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008809:Mice, Inbred C3H; D008920:Misonidazole / Q000378:metabolism; D004364:Pharmaceutical Preparations / Q000032:analysis*; D014867:Water / Q000378:metabolism
D004364:Pharmaceutical Preparations; D014867:Water; D008920:Misonidazole; D003903:Deuterium