title: Methylation of hen erythrocyte DNA.
abstract: We have analysed the 5-methylcytosine content of hen erythrocyte DNA and found it to be lower than that of DNA from other chick tissues analysed. Erythrocyte DNA is also a better substrate for DNA methylase having a five-fold lower Km than DNA from white blood cells. This is probably because it contains a large number of hemimethylated sites. Thus the inverse correlation between methylation and gene expression does not apply to the chick red blood cell.
authors.affiliation: Department of Biochemistry, University of Glasgow, Scotland.
authors.forename: R L
authors.initials: RL
authors.lastname: Adams
authors.forename: C L
authors.initials: CL
authors.lastname: So
mesh_terms: D044503:5-Methylcytosine; D000818:Animals; D002645:Chickens; D003596:Cytosine / Q000031:analogs & derivatives / Q000032:analysis; D004247:DNA / Q000032:analysis / Q000097:blood*; D004912:Erythrocytes / Q000032:analysis*; D005260:Female; D007962:Leukocytes / Q000032:analysis; D008099:Liver / Q000032:analysis; D008168:Lung / Q000032:analysis; D008745:Methylation; D008780:Methyltransferases / Q000378:metabolism; D051379:Mice; D013154:Spleen / Q000032:analysis; D013379:Substrate Specificity
chemical_list: D044503:5-Methylcytosine; D003596:Cytosine; D004247:DNA; D008780:Methyltransferases