Interleukin 1 alpha and beta in psoriatic skin: enzymoimmunoassay, immunoblot studies and effect of systemic retinoids.
Interleukin 1 alpha (IL-1 alpha) and interleukin 1 beta (IL-1 beta) proteins were studied by enzymoimmunoassay (EIA) and Immunoblot analysis in the 10,000 g supernatant of normal and psoriatic (lesional and nonlesional) human skin specimens. By EIA IL-1 alpha was the principal form detected in all the specimens, which contrasts with the predominance of IL-1 beta in human blood monocytes. In psoriatic plaques relatively less IL-1 alpha and more IL-1 beta were detected. On Immunoblot analysis the mature form (17 kD) was not detected in normal skin, which showed only 52-kD immunoreactive forms. In contrast the 17-kD form was found in psoriatic skin. This indicates either a distinct processing of IL-1 molecules or a contribution of inflammatory cells infiltration to the IL-1 pool in psoriatic plaques. During systemic retinoids therapy the amount of both IL-1 species decreased in lesional and nonlesional psoriatic skin.
Gruaz
D
D
Département de Médecine, Hôpital Cantonal Universitaire, Genève, Suisse.
Didierjean
L
L
Grassi
J
J
Frobert
Y
Y
Dayer
J M
JM
Saurat
J H
JH
D004591:Electrophoresis, Polyacrylamide Gel; D004817:Epidermis / Q000032:analysis; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D007375:Interleukin-1 / Q000032:analysis*; D008297:Male; D011565:Psoriasis / Q000188:drug therapy / Q000378:metabolism*; D012176:Retinoids / Q000494:pharmacology*; D012867:Skin / Q000032:analysis; D012967:Sodium Dodecyl Sulfate
D007375:Interleukin-1; D012176:Retinoids; D012967:Sodium Dodecyl Sulfate