Endotoxin protection against pulmonary oxygen toxicity and its reversal by acetyl salicylic acid: role of eicosanoid production by broncho-alveolar lavage cells.
Small doses of endotoxin markedly increase the survival rate of adult rats exposed to 98% oxygen for periods that are normally lethal. The lysine salt of acetyl salicylic acid (L-ASA) partially reverses this protective effect of endotoxin. In this pilot study we investigated the level of eicosanoid production by broncho-alveolar lavage (BAL) cells and found that BAL cells of endotoxin protected rats, present in abundance, have an equal or increased capacity of HHT, 15-HETE, 12-HETE, LTB4 and 5-HETE production. These data suggest that production of the lipoxygenase products by BAL cells does not seem to play an important role in the pathogenesis of pulmonary oxygen toxicity. We did not find any indication for the occurrence of shunting of arachidonic acid metabolism to the lipoxygenase pathway as an explanation for the reversal of endotoxin's protective action by L-ASA.
Klein
J
J
Department of Anesthesia, Erasmus University Rotterdam, The Netherlands.
Vermeer
M A
MA
Zijlstra
F J
FJ
Vincent
J E
JE
Tak
C J
CJ
D000818:Animals; D001241:Aspirin / Q000494:pharmacology*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000378:metabolism*; D004537:Eicosanoic Acids / Q000096:biosynthesis*; D004731:Endotoxins / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D008168:Lung / Q000187:drug effects*; D008297:Male; D010100:Oxygen / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains
D004537:Eicosanoic Acids; D004731:Endotoxins; D001241:Aspirin; D010100:Oxygen