Physical linkage of mouse lambda genes by pulsed-field gel electrophoresis suggests that the rearrangement process favors proximate target sequences.
The first complete map of a mammalian immunoglobulin gene locus is presented. Mouse lambda genes were mapped by pulsed-field gel electrophoresis. The gene order is V2-Vx-C2-C4-V1-C3-C1. The distance between V2 or Vx and the C2-C4 cluster is 74 or 55 kilobases (kb), respectively, whereas that between V1 and C3-C1 is only 19 kb; V2 and C3-C1 are at least 190 kb apart. Thus, the distances between the lambda subloci are inversely proportional to their frequencies of rearrangement. The related gene lambda 5 is not within the 500 kb of the lambda locus mapped here.
Storb
U
U
Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.
Haasch
D
D
Arp
B
B
Sanchez
P
P
Cazenave
P A
PA
Miller
J
J
D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D015342:DNA Probes; D004587:Electrophoresis, Agar Gel; D015328:Gene Rearrangement, B-Lymphocyte, Light Chain; D008040:Genetic Linkage; D051379:Mice; D015183:Restriction Mapping
D015342:DNA Probes