Development and characterization of monoclonal antibodies reactive with paraquat.
A set of three anti-paraquat monoclonal antibodies(MoAbs), named APM-1, APM-2 and APM-3, has been isolated. In order to evaluate the ability of these MoAbs to recognize various kinds of bipyridyl herbicides and similar congeners of paraquat, a competition enzyme-linked immunosorbent assay (ELISA) using avidin-biotin complex (ABC) was developed. All three antibodies strongly recognized paraquat and slightly did the other analogs. These three MoAbs are therefore advantageous to a toxicological study of paraquat and of its localization in tissues.
Nagao
M
M
Department of Legal Medicine, Hokkaido University School of Medicine, Sapporo, Japan.
Takatori
T
T
Wu
B
B
Terazawa
K
K
Gotouda
H
H
Akabane
H
H
D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001360:Avidin; D001710:Biotin; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006241:Haptens; D006825:Hybridomas / Q000276:immunology; D010269:Paraquat / Q000031:analogs & derivatives / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006241:Haptens; D001360:Avidin; D001710:Biotin; D010269:Paraquat