20-Hydroxyecdysone acts in the male pupa to commit accessory glands toward trehalase production in the adult mealworm beetle (Tenebrio molitor).
During postecdysial adult maturation, the bean-shaped accessory reproductive glands (BAGs) of adult male mealworm beetles produce increasing amounts of trehalase. In order to determine when the BAGs become competent to produce trehalase, we transplanted pupal BAGs into 0-day female adults. After 8 days, trehalase activity had increased in BAGs from 4- and 5-day pupae (at the time of the pupal ecdysteroid peak) but not in those from 1- and 2-day pupae (before the ecdysteroid peak). BAGs from 0- and 2-day pupae were exposed to 20-hydroxyecdysone in vitro before implantation into 0-day female adults. Increase in trehalase activity was dose dependent. Both dose (ED50, 5 x 10(-6) M) and exposure time (greater than 6 hr) of hormone required are greater for commitment than for acceleration of pupal cell cycling (T. Yaginuma, H. Kai, and G.M. Happ, 1988, Dev. Biol. 126, 173-181). Since trehalase activity increased markedly in isolated adult male abdomens, factors from the cephalic and thoracic centers are not required to sustain trehalase production in the adult BAGs.
Yaginuma
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Department of Zoology, University of Vermont, Burlington 05405.
Happ
G M
GM
D000005:Abdomen / Q000033:anatomy & histology; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004441:Ecdysterone / Q000494:pharmacology / Q000502:physiology*; D005837:Genitalia, Male / Q000187:drug effects / Q000201:enzymology*; D008026:Ligation; D008297:Male; D009490:Neurosecretory Systems / Q000502:physiology; D011679:Pupa / Q000378:metabolism / Q000502:physiology; D013712:Tenebrio / Q000502:physiology*; D014198:Trehalase / Q000096:biosynthesis* / Q000378:metabolism
D004441:Ecdysterone; D014198:Trehalase