Effect of DNA supercoiling on in vitro transcription from the adenovirus early region 4.
The effect of DNA supercoiling on in vitro transcription from the early region 4 (E4) promoter of adenovirus type 5 (Ad5) has been investigated by using a cell-free transcription system that maintains supercoiled DNA templates. The supercoiled DNAs yield several-fold higher levels of E4 transcripts and more faithfully reflect the regulation of in vivo transcription than the linear DNAs.
Handa
H
H
Department of Bacteriology, University of Tokyo Faculty of Medicine, Japan.
Watanabe
H
H
Suzuki
Y
Y
Hirose
S
S
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D012831:Bombyx; D002474:Cell-Free System; D004278:DNA, Superhelical / Q000494:pharmacology*; D005088:Exocrine Glands; D005786:Gene Expression Regulation; D066298:In Vitro Techniques; D009154:Mutation; D011401:Promoter Regions, Genetic / Q000187:drug effects; D014158:Transcription, Genetic / Q000187:drug effects*
D004278:DNA, Superhelical