Temperature-dependent CD and NMR studies on a synthetic oligonucleotide containing a B-Z junction at high salt.
It is now accepted that two or more conformations may exist within the same DNA molecule, thereby generating conformational junctions. The presence of B-Z junctions between right- and left-handed DNA conformations has been detected in plasmids by a number of techniques. Preliminary characterization of the first example of a B-Z junction is a short DNA oligonucleotide has recently been reported [Sheardy, R. D. (1988) Nucleic Acids Res. 16, 1153-1167]. We report additional CD and NMR data that support the existence of the junction in this model oligomer. These studies indicate that only three base pairs are involved in the junction and only one of these is dramatically distorted. Furthermore, the NMR saturation-transfer experiments suggest the junction's internal motion is temperature dependent.
Sheardy
R D
RD
Department of Chemistry, Pennsylvania State University, Hazleton 18201.
Winkle
S A
SA
D002942:Circular Dichroism; D004247:DNA; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis; D009994:Osmolar Concentration; D012492:Salts; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D009838:Oligodeoxyribonucleotides; D012492:Salts; D004247:DNA