Role of recBC function in formation of chromosomal rearrangements: a two-step model for recombination.
The role of recBC functions has been tested for three types of chromosomal recombination events: (1) recombination between direct repeats to generate a deletion, (2) recombination between a small circular fragment and the chromosome, and (3) recombination between inversely oriented repeats to form an inversion. Deletion formation by recombination between direct repeats, which does not require a fully reciprocal exchange, is independent of recBC function. Circle integration and inversion formation are both stimulated by the recBC function; these events require full reciprocality. The results suggest that half-reciprocal exchanges can occur without recBC, but recBC functions greatly stimulate completion of a fully reciprocal exchange. We propose that chromosomal recombination is a two-step process, and recBC functions are primarily required for the second step.
Mahan
M J
MJ
Department of Biology, University of Utah, Salt Lake City 84112.
Roth
J R
JR
D002872:Chromosome Deletion; D007446:Chromosome Inversion; D003470:Culture Media; D015321:Gene Rearrangement; D005798:Genes, Bacterial; D008040:Genetic Linkage; D006639:Histidine / Q000235:genetics; D008957:Models, Genetic; D011995:Recombination, Genetic; D009626:Terminology as Topic; D014161:Transduction, Genetic
D003470:Culture Media; D006639:Histidine