Phenolic glycolipid-1 from M. leprae inhibits oxygen free radical production by human mononuclear cells.
We studied the effect of PGL1, a phenolic glycolipid unique to Mycobacterium leprae, on the activation of the phagocyte oxidative respiratory burst, by measuring the chemiluminescence (CL) generated by normal mononuclear cells. PGL1 induced a decrease in oxygen free radical production stimulated by mycobacteria (M. leprae, BCG and M. kansasii) or by phorbol myristate acetate, but did not prevent the binding or ingestion of fluorescein-conjugated mycobacteria. In contrast, mycoside A from M. kansasii, a structurally related compound, did not alter the CL response. In addition, treatment of M. leprae with anti-PGL1 antibodies failed to restore the response to this microorganism. PGL1 could act as an oxygen species scavenger and protect M. leprae from killing by toxic oxygen metabolites.
Launois
P
P
Immunologie cellulaire, Institut Pasteur de Dakar.
Blum
L
L
Dieye
A
A
Millan
J
J
Sarthou
J L
JL
Bach
M A
MA
D000328:Adult; D000907:Antibodies, Bacterial / Q000494:pharmacology; D000942:Antigens, Bacterial; D005609:Free Radicals; D006017:Glycolipids / Q000276:immunology / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D008163:Luminescent Measurements; D009161:Mycobacterium / Q000276:immunology; D009163:Mycobacterium bovis / Q000276:immunology; D009166:Mycobacterium leprae / Q000276:immunology; D010100:Oxygen / Q000097:blood*; D010587:Phagocytosis / Q000187:drug effects; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D005609:Free Radicals; D006017:Glycolipids; C045169:phenolic glycolipid I, Mycobacterium leprae; C051618:mycoside A; D013755:Tetradecanoylphorbol Acetate; D010100:Oxygen