Linear chromosome of Borrelia burgdorferi.
The DNA organization of several European and American isolates of Borrelia burgdorferi, the aetiological agent of Lyme disease, was analysed in pulse-field agarose gel electrophoresis. The results of in situ cell lysis in agarose plugs demonstrated a unique arrangement for the DNA of this spirochete. The chromosome of Borrelia behaved as a eukaryotic linear chromosome with a size of around 1,000 kb. The genome also comprised several circular and linear plasmids which varied in size from 15 to 60 kb.
Baril
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Unité des Leptospires, Institut Pasteur, Paris.
Richaud
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C
Baranton
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Saint Girons
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IS
D015748:Borrelia burgdorferi Group / Q000235:genetics*; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D066298:In Vitro Techniques; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D010957:Plasmids / Q000235:genetics; D015183:Restriction Mapping
D004269:DNA, Bacterial