A calcium-dependent antibody for identification and purification of recombinant proteins.
We report a straightforward methodology for purification of recombinant proteins by incorporating a short hydrophilic peptide marker segment at their N-termini. A calcium-dependent antibody that reacts primarily with the first three amino acids of this peptide segment was used to affinity purify the fusion proteins in a single chromatographic step. The marker peptide could subsequently be removed by proteolysis with the enzyme enterokinase.
Prickett
K S
KS
Immunex Corporation, Seattle, WA 98101.
Amberg
D C
DC
Hopp
T P
TP
D000911:Antibodies, Monoclonal; D002118:Calcium; D002846:Chromatography, Affinity / Q000379:methods*; D004765:Enteropeptidase; D005822:Genetic Vectors; D007377:Interleukin-3 / Q000235:genetics / Q000302:isolation & purification*; D015847:Interleukin-4 / Q000235:genetics / Q000302:isolation & purification*; D010957:Plasmids; D011994:Recombinant Proteins / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae
D000911:Antibodies, Monoclonal; D007377:Interleukin-3; D011994:Recombinant Proteins; D015847:Interleukin-4; D004765:Enteropeptidase; D002118:Calcium