An ultrathin sectioning alignment tool with application to cell monolayers.
Details are given concerning the construction and use of a simple tool to help align a specimen block face in the vertical axis for subsequent ultrathin sectioning. Further instructions are given for the preparation of cell monolayers for ultrathin sectioning. The advantage is simple, repetitive and quick alignment of the specimen block face for ultrathin sectioning.
Cramer
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Pathology and Toxicology Research, Upjohn Company, Kalamazoo, Michigan 49001.
D000818:Animals; D046508:Culture Techniques / Q000379:methods*; D008854:Microscopy, Electron / Q000379:methods*; D008867:Microtomy / Q000295:instrumentation* / Q000379:methods