Enzymatic deacylations of esterified saccharides--III. Comparison of de-esterifications by serum esterases from different sources.
1. 14C-labelled methyl 2,6-di-O-pivaloyl-alpha-D-glucopyranoside (1) was used as a substrate for esterases from rabbit, guinea pig, mouse, donkey, pig, horse, sheep and human sera. 2. Stepwise de-esterification of the diester substrate 1 occurred with rabbit, guinea pig and mouse serum. Data on time-course experiments and kinetic data are reported. 3. The use of donkey, pig, horse, sheep and human serum led to the migration of the 2-O-pivaloyl group in substrate 1 to the position 4- in the sugar molecule, followed by stepwise de-esterifications of both 1 and the newly formed methyl 4,6-di-O-pivaloyl-alpha-D-glucopyranoside (4). A report is given on the time-course experiments.
Tomić
S
S
Department of Radioimmunology, Institute of Immunology, Zagreb, Yugoslavia.
Sesartić
L
L
Tomasić
J
J
D000818:Animals; D004950:Esterases / Q000097:blood*; D006168:Guinea Pigs; D006736:Horses; D006801:Humans; D006868:Hydrolysis; D007700:Kinetics; D051379:Mice; D011817:Rabbits; D012756:Sheep; D013045:Species Specificity; D013552:Swine
D004950:Esterases