title: Confocal scanning microscopy: three-dimensional biological imaging.
abstract: Confocal scanning optical microscopy, arguably the most significant in biological light microscopy in this decade, enables one to obtain quantitative non-invasive optical sections through labelled biological specimens, virtually free from out-of-focus blur. A set of these optical sections collected at a series of focal levels through an object constitutes a three-dimensional image which may then be processed digitally for display as a computer reconstruction, a stereo pair or an animation sequence.
authors.forename: D
authors.initials: D
authors.lastname: Shotton
authors.forename: N
authors.initials: N
authors.lastname: White
mesh_terms: D000818:Animals; D005347:Fibroblasts / Q000166:cytology; D007091:Image Processing, Computer-Assisted; D008168:Lung / Q000166:cytology; D008853:Microscopy / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods