title: Immobilization of enzyme by microencapsulation and application of the encapsulated enzyme in the catalysis.
abstract: Microencapsulation of lipase (Pseudomonas fluorescens) was carried out using (W/O)/W two-phase emulsion technique. Polystyrene (PS) and Styrene-Butadiene Rubber (SBR) were utilized as wall materials either separately or in mixture. A particular composition of 2:1 PS-SBR yielded homogeneous and tough wall structure, resilient to the impact and tight confinement of enzyme macromolecules. Performance of the encapsulated enzyme was evaluated employing the hydrolysis of triacetin (triglyceride of acetic acid) as a model substrate of the enzyme catalysis. A mathematical model was developed to simulate the behaviour of hydrolysis, which was derived under the assumption that the diffusion of small molecules (substrate and products) through the wall of microcapsules plays a dominant role to the reaction rate. Inhibition of the reaction by the decreasing pH due to the release of acetic acid was also taken into account. The calculated values agreed quite well with the observed data.
authors.affiliation: Department of Chemical Engineering, Tokyo University of Agriculture and Technology, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Iso
authors.forename: T
authors.initials: T
authors.lastname: Shirahase
authors.forename: S
authors.initials: S
authors.lastname: Hanamura
authors.forename: S
authors.initials: S
authors.lastname: Urushiyama
authors.forename: S
authors.initials: S
authors.lastname: Omi
mesh_terms: D004339:Drug Compounding; D004800:Enzymes, Immobilized; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000008:administration & dosage*; D008433:Mathematics; D014215:Triacetin / Q000378:metabolism
chemical_list: D004800:Enzymes, Immobilized; D008049:Lipase; D014215:Triacetin