title: Small inhibitory cerebellar interneurons grow in a perpendicular orientation to granule cell neurites in culture.
abstract: When explants or reaggregates of small neurons from early postnatal mouse cerebella are plated on a mixture of laminin and poly-D-lysine, one observes small cells with an orientation of processes largely perpendicular to the direction of granule cell neurites after several days. These cells first have a bipolar morphology and then elaborate a rich dendritic arbor-like structure opposite a long, thin axon-like process. Several lines of evidence suggest that these cells are the small inhibitory interneurons of the cerebellar cortex: They take up GABA, express high levels of the embryonic form of N-CAM, do not express L1, the oligodendrocyte marker O4, or the glial marker vimentin, and display ultrastructural features reminiscent of stellate and/or basket cells in vivo. These observations suggest that the elaboration of directional positioning of small inhibitory interneurons can be studied in culture, thus offering the possibility to elucidate the cellular and molecular mechanisms underlying the orientation of particular neural cells with regard to others.
authors.affiliation: Department of Neurobiology, University of Heidelberg, Federal Republic of Germany.
authors.forename: A
authors.initials: A
authors.lastname: Hekmat
authors.forename: V
authors.initials: V
authors.lastname: Künemund
authors.forename: G
authors.initials: G
authors.lastname: Fischer
authors.forename: M
authors.initials: M
authors.lastname: Schachner
mesh_terms: D000818:Animals; D001369:Axons / Q000648:ultrastructure*; D002449:Cell Aggregation; D002455:Cell Division; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology*; D005455:Fluorescent Antibody Technique; D007395:Interneurons / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D007797:Laminin / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron; D009924:Organ Culture Techniques; D011107:Polylysine / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D007797:Laminin; D011107:Polylysine; D005680:gamma-Aminobutyric Acid