title: Rates of pyruvate utilization and lactate formation by lymphocytes from young and aged rats.
abstract: The rates of pyruvate utilization and lactate formation were measured in lymphocytes from young (newly weaned), adult (3-month old) and aged (15-month old) rats. Lymphocytes obtained from mesenteric lymph nodes were incubated at 37 degrees C for 1 h. The conversion of pyruvate to lactate accounted for 92.5% of the total pyruvate utilized by lymphocytes from young rats and was 34.2% and 44.5% for adult and aged rats, respectively. When lymphocytes were stimulated with concanavalin A there was an increase of 65.6% and 37.1% in the rates of pyruvate utilization by lymphocytes from young and adult rats, respectively. No changes in pyruvate utilization were observed in response to concanavalin A in lymphocytes from aged rats.
authors.affiliation: Departamento de Fisiologia e Biofísica, Universidade de São Paulo, Brasil.
authors.forename: M A
authors.initials: MA
authors.lastname: Serrano
authors.forename: R
authors.initials: R
authors.lastname: Curi
mesh_terms: D000375:Aging; D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology*; D066298:In Vitro Techniques; D007773:Lactates / Q000096:biosynthesis*; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D011773:Pyruvates / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D007773:Lactates; D011773:Pyruvates; D003208:Concanavalin A