Isolation, purification, and characterization of fucose-containing sulfated polysaccharides from the brown seaweed Ecklonia kurome and their blood-anticoagulant activities.
A sulfated polysaccharide fraction, obtained from the hot-water extract of the brown seaweed, Ecklonia kurome by removing laminaran and the major part of alginic acid, gave sulfated polysaccharides (B-I, B-II, C-I, and C-II) by both anion-exchange chromatography on a column of Ecteola-cellulose and by fractional precipitation with ethanol containing 0.3% calcium acetate, and then by gel-filtration chromatography on a Sepharose 4B column. B-I and B-II are composed of fucose, galactose, mannose, xylose, glucuronic acid, and ester sulfate in the approximate molar ratios of 1.00:0.36:0.48:1.08:1.85:2.35 and 1.00:0.81:0.18:0.45:0.61:2.00, respectively. C-I and C-II are composed of fucose, galactose, glucuronic acid, and ester sulfate in approximate molar ratios of 1.00:0.03:0.03:1.61 and 1.00:0.19:0.07:1.48, respectively. Blood-anticoagulant activities with respect to activated partial thromboplastin time (APTT) were approximately 24, 19, 81, and 85% of that of heparin for B-I, B-II, C-I, and C-II, respectively. All the polysaccharides showed slight antithrombin activity. No antifactor Xa activity was observed for any of the polysaccharides.
Nishino
T
T
Department of Biophysics, School of Hygienic Sciences, Kitasato University, Kanagawa, Japan.
Yokoyama
G
G
Dobashi
K
K
Fujihara
M
M
Nagumo
T
T
D000818:Animals; D000925:Anticoagulants / Q000302:isolation & purification*; D002417:Cattle; D002850:Chromatography, Gel; D005643:Fucose / Q000032:analysis*; D006801:Humans; D010314:Partial Thromboplastin Time; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology; D012624:Seaweed; D013464:Sulfuric Acids / Q000032:analysis; D013917:Thrombin / Q000502:physiology; D013918:Thrombin Time
D000925:Anticoagulants; D011134:Polysaccharides; D013464:Sulfuric Acids; D005643:Fucose; D013917:Thrombin