title: Degradation of bovine C3 by serine proteases from parasites Hypoderma lineatum (Diptera, Oestridae).
abstract: Purified enzymes of Hypoderma lineatum (Insecta, Oestridae), were assayed for their proteolytic activity on bovine C3 in normal cattle sera. The products of cleavage by these serine proteases (hypodermins A, B, and C), were analysed by electrophoresis in SDS polyacrylamide gels followed by immunoblotting. The enzymatic attack was initially directed at the alpha polypeptide chain by hypodermin A at a concentration of 1 micrograms/ml of serum and by hypodermin B at 5 micrograms/ml. The generated peptides differed in their molecular size from those produced during natural degradation of C3 in a control serum by physiologically relevant enzymes. Hypodermin A, at a concentration of 1 microgram/ml, also caused a cleavage of the beta chain. At 5 micrograms/ml, hypodermin A induced total degradation of the C3 molecule. Hypodermin B (5 micrograms/ml) starts splitting C3 near cleavage sites of factor I. Bovine C3 appears to be highly sensitive to hypodermins A and B in normal sera. Apparent molecular sizes and alignment of the bovine C3 cleavage products are presented schematically. Hypodermin C, a collagenolytic enzyme, had no effect on C3 in normal sera. The biological consequences for the immunopathological reactions associated with hypodermosis are discussed.
authors.affiliation: I.N.R.A. - C.R. de Tours-Nouzilly, Station de Pathologie Aviaire et de Parasitologie, Monnaie, France.
authors.forename: C
authors.initials: C
authors.lastname: Boulard
mesh_terms: D000818:Animals; D002417:Cattle; D003176:Complement C3 / Q000378:metabolism*; D004175:Diptera / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D007106:Immune Sera / Q000276:immunology; D015151:Immunoblotting; D012697:Serine Endopeptidases / Q000378:metabolism*
chemical_list: D003176:Complement C3; D007106:Immune Sera; D012697:Serine Endopeptidases