Detection of Plasmodium falciparum using a cloned DNA probe: a simple procedure suitable for field application.
A simple procedure was developed for spotting blood samples directly onto nylon membrane filter, without the necessity to treat samples with pronase or proteinase K, followed by hybridizing with 32P-labelled DNA probe, pUNK1-45. This probe detected specifically P. falciparum DNA and did not cross react with DNA from man, P. knowlesi, P. chabaudi or P. cynomolgi. The probe was sensitive to detect a parasitemia of 0.001% in 20 microliters of blood.
Boonsaeng
V
V
Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand.
Chansiri
K
K
Vilasineekul
P
P
Wilairat
P
P
Panyim
S
S
D000818:Animals; D015342:DNA Probes; D004812:Epidemiologic Methods; D006801:Humans; D008288:Malaria / Q000097:blood / Q000175:diagnosis*; D009693:Nucleic Acid Hybridization; D010761:Phosphorus Radioisotopes; D010963:Plasmodium falciparum / Q000302:isolation & purification*
D015342:DNA Probes; D010761:Phosphorus Radioisotopes