Co-culture of fibroblasts and hepatic parenchymal cells induces metabolic changes and formation of a three-dimensional structure.
Co-culturing of tendon fibroblasts and liver parenchymal cells in Williams' medium E supplemented with fetal bovine serum, hormones, and L-ascorbic acid 2-phosphate, a long acting vitamin C derivative, resulted in formation of three-dimensional structure. Both growth of fibroblasts and their production of collagen were inhibited, however production of albumin by the hepatocytes was much better preserved than when individual cells were cultured separately, indicating epithelial-mesenchymal interactions stimulate reorganization of the liver-like tissue from isolated cells.
Senoo
H
H
Department of Anatomy, School of Medicine, Tokyo Medical and Dental University, Japan.
Tsukada
Y
Y
Sato
T
T
Hata
R
R
D000125:Achilles Tendon; D000418:Albumins / Q000096:biosynthesis*; D000818:Animals; D001205:Ascorbic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D002452:Cell Count; D002478:Cells, Cultured; D003094:Collagen / Q000032:analysis / Q000096:biosynthesis*; D003470:Culture Media; D004247:DNA / Q000032:analysis; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D008099:Liver / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains
D000418:Albumins; D003470:Culture Media; C001956:ascorbic acid 2-sulfate; D003094:Collagen; D004247:DNA; D001205:Ascorbic Acid