title: Determination of formaldehyde and acetaldehyde in mainstream cigarette smoke by high-performance liquid chromatography.
abstract: A method is described for the determination of formaldehyde and acetaldehyde in mainstream cigarette smoke. This involved the collection and reaction of the aldehydes with 2,4-dinitrophenylhydrazine in aqueous acetonitrile. The high-performance liquid chromatographic separation and measurement of the various components directly in this reaction solution eliminated the need for a clean-up stage. Cigarette yields of greater than 5 micrograms of formaldehyde and 50 micrograms of acetaldehyde could be determined to estimated relative standard deviations of 0.07 and 0.05, respectively.
authors.forename: P R
authors.initials: PR
authors.lastname: Houlgate
authors.forename: K S
authors.initials: KS
authors.lastname: Dhingra
authors.forename: S J
authors.initials: SJ
authors.lastname: Nash
authors.forename: W H
authors.initials: WH
authors.lastname: Evans
mesh_terms: D000079:Acetaldehyde / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005557:Formaldehyde / Q000032:analysis*; D010947:Plants, Toxic; D012906:Smoke / Q000032:analysis*; D014026:Nicotiana / Q000032:analysis*
chemical_list: D012906:Smoke; D005557:Formaldehyde; D000079:Acetaldehyde