Colchicine effect on B-50/GAP43 phosphoprotein localization in rat dorsal root ganglion explants.
In rat embryonic dorsal root ganglion explants stimulated by nerve growth factor, the neuron-specific phosphoprotein B-50 (GAP43) is primarily localized in the distal portion of outgrowing neurites. Addition of colchicine leads to a decrease in total amount of B-50 and a marked redistribution in the neurons. The data underscore the role of axonal transport in the concentration of B-50 in growth cones of growing neurites.
Schmidt-Michels
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Division of Molecular Neurobiology, Rudolf Magnus Institute for Pharmacology, University of Utrecht, The Netherlands.
Edwards
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PM
Oestreicher
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Gispen
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D000818:Animals; D003078:Colchicine / Q000494:pharmacology*; D046508:Culture Techniques; D019922:GAP-43 Protein; D005727:Ganglia, Spinal / Q000187:drug effects* / Q000378:metabolism; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000378:metabolism*; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D011863:Radioimmunoassay; D051381:Rats
D019922:GAP-43 Protein; D008565:Membrane Proteins; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D003078:Colchicine