CA-125 ovarian cancer associated antigen in cancer and pregnancy: interpretation of enzyme immunoassay and immunoradiometric assay.
Serum CA-125 concentrations were determined using both immunoradiometric assay (IRMA) and enzyme immunoassay (EIA) in 67 patients with various diseases including cancer and in 100 pregnant women with respect to the stage of pregnancy. Correlation of values obtained by the two methods was good except for the first and second trimester of pregnancy. However, IRMA concentrations were 80-200% higher than those obtained by EIA, the larger differences being observed in the critical range of 35-100 U/mL. Therefore, the alternate use of the two methods should be avoided and the cutoff levels should be redefined for each procedure.
Touitou
Y
Y
Department of Biochemistry, Faculté de Médecine Pittié-Salpêtrière, Paris, France.
Bogdan
A
A
Darbois
Y
Y
D000328:Adult; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000032:analysis*; D001943:Breast Neoplasms / Q000097:blood; D003110:Colonic Neoplasms / Q000097:blood; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D009369:Neoplasms / Q000097:blood*; D010051:Ovarian Neoplasms / Q000097:blood*; D011247:Pregnancy / Q000097:blood*; D011261:Pregnancy Trimester, First; D011262:Pregnancy Trimester, Second; D011263:Pregnancy Trimester, Third; D011863:Radioimmunoassay
D015295:Antigens, Tumor-Associated, Carbohydrate