title: Effects of electrical stimulation on the post-mortem biochemical changes and texture of broiler pectoralis muscle.
abstract: A study was conducted to determine the influence of electrical stimulation (50,200, or 350 V ac) on biochemical and textural changes in broiler breast muscle. Sixty-four broilers were stunned (50 V ac) prior to kill, and all but a control group were pulse stimulated during bleeding. After processing, carcasses were held in 10-C water for 1 h, then in 2-C ice/water slush for 1 h prior to muscle removal at 2 h post-mortem (PM). Bagged samples were held at 2 C for 24 h, then cooked. The pH, R value (ratio of adenine nucleotides to inosine nucleotides), cook yield, fluids and solids lost (F&S), and objective texture were measured. In addition, 16 broilers processed in the same manner were used in producing a profile of sarcoplasmic protein/enzyme changes in the breast muscle by cation exchange fast protein-liquid chromatography at 10 min, 2, and 24 h PM. The R values and soluble protein were also determined. Stimulation at 200 and 350 V accelerated the onset of rigor noted by lower pH values at 10 min and 1 h PM, and higher R values at 2 h PM. Muscle stimulated at 350 V exhibited the lowest cook yield and highest percentage of F&S lost, suggesting both the loss of functional properties and muscle integrity due to this treatment. All control and stimulated samples exhibited shear values in excess of what would be considered tender. Hardness and chewiness values increased as stimulation voltage levels increased. Only one of the seven principal chromatographic peaks decreased in response to increased electrical stimulation.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: USDA, Russell Research Center, Athens, Georgia 30613.
authors.forename: C E
authors.initials: CE
authors.lastname: Lyon
authors.forename: C E
authors.initials: CE
authors.lastname: Davis
authors.forename: J A
authors.initials: JA
authors.lastname: Dickens
authors.forename: C M
authors.initials: CM
authors.lastname: Papa
authors.forename: J O
authors.initials: JO
authors.lastname: Reagan
mesh_terms: D000227:Adenine Nucleotides / Q000032:analysis; D000818:Animals; D002645:Chickens / Q000033:anatomy & histology*; D002852:Chromatography, Ion Exchange; D004558:Electric Stimulation; D006863:Hydrogen-Ion Concentration; D007292:Inosine Nucleotides / Q000032:analysis; D008297:Male; D008460:Meat / Q000592:standards*; D010369:Pectoralis Muscles / Q000032:analysis / Q000033:anatomy & histology* / Q000378:metabolism
chemical_list: D000227:Adenine Nucleotides; D007292:Inosine Nucleotides