title: Macrophage differentiation inducing factor from human monocytic cells is equivalent to murine leukemia inhibitory factor.
abstract: Human macrophage differentiation inducing factor (DIF) can induce differentiation of human myeloid leukemic cells into macrophage-like cells in vitro. A procedure is described for purification of DIF from serum-free human monocytic leukemia THP-1 cell-conditioned medium. The procedure included concentration of a conditioned medium by ultrafiltration, lentil lectin-Sepharose affinity chromatography, and fast protein liquid chromatography using Mono S and Mono Q. DIF-A of pI 9.0 and DIF-B of pI 8.8 were obtained after a final purification with a Mono Q column, and both DIF gave a single peak with a molecular weight of approximately 51,000 determined by gel chromatography. NH2-terminal amino acid analysis of DIF-A showed a noticeable homology with murine leukemia inhibitory factor. Human DIF-A was found to induce maturation of human and murine leukemic cells into both macrophage-like cells with nitro blue tetrazolium reducing activity and phagocytic cells, but was found to suppress proliferation of these leukemic cells.
authors.affiliation: Medical Science Laboratory, Asahi Chemical Industry Co., Ltd., Shizuoka-ken, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Abe
authors.forename: M
authors.initials: M
authors.lastname: Murakami
authors.forename: T
authors.initials: T
authors.lastname: Sato
authors.forename: M
authors.initials: M
authors.lastname: Kajiki
authors.forename: M
authors.initials: M
authors.lastname: Ohno
authors.forename: R
authors.initials: R
authors.lastname: Kodaira
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005293:Ferritins / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D007525:Isoelectric Focusing; D007942:Leukemia, Experimental / Q000503:physiopathology; D008222:Lymphokines / Q000302:isolation & purification* / Q000494:pharmacology; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D009000:Monocytes / Q000502:physiology*
chemical_list: D008222:Lymphokines; C020391:acidic isoferritin; C050979:monocyte-macrophage differentiation factor; D005293:Ferritins