Complete 1H- and 13C-n.m.r. assignments for two sulphated oligosaccharide alditols of hen ovomucin.
The complete 1H- and 13C-n.m.r. assignments for beta-D-Galp-(1----4)-beta-D-GlcpNAc-6-SO3H-(1----6)-[beta-D-Galp-(1----3 )]- D-GalNAcol and alpha-NeuAcp-(2----3)-beta-D-Galp-(1----3)-[beta-D-Galp-(1----4)-b eta-D- GlcpNAc-6-SO3H-(1----6)]-D-GalNAcol were made by a combination of 2-D correlation experiments (Relayed-Cosy; and 13C,1H Correlation-shift n.m.r. spectroscopy), and 1-D n.m.r. spectroscopy. The results illustrate the ability of these methods to locate sulphate and NeuAc groups in anionic mucinous glycoproteins.
Strecker
G
G
Laboratoire de Chimie Biologique, Unité Associée au CNRS 217, Université des Sciences et Techniques de Lille Flandres-Artois, Villeneuve d'Ascq, France.
Wieruszeski
J M
JM
Martel
C
C
Montreuil
J
J
D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002247:Carbon Isotopes; D002645:Chickens; D004527:Egg Proteins; D005260:Female; D006859:Hydrogen; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000302:isolation & purification; D010059:Ovomucin; D013464:Sulfuric Acids / Q000302:isolation & purification
D002247:Carbon Isotopes; D004527:Egg Proteins; D009844:Oligosaccharides; D013464:Sulfuric Acids; D010059:Ovomucin; D006859:Hydrogen