title: A phosphodiester bridge between two arabinose residues as a structural element of an extracellular glycoprotein of Volvox carteri.
abstract: The sulphated glycoprotein SSG 185 is the monomeric precursor of a highly aggregated structural element in the extracellular matrix of the multicellular green alga Volvox carteri. A phosphodiester of arabinose was isolated from a saccharide fragment of SSG 185. The structure of this phosphodiester was investigated by methylation analysis, 13C-NMR, photometric methods and enzymatic assays and identified as D-Araiota-5-phospho-5-D-Araiota. The function of this phosphodiester bridge as a crosslink of different carbohydrate chains in SSG 185 is discussed.
authors.affiliation: Lehrstuhl Biochemie I der Universität, Regensburg, Federal Republic of Germany.
authors.forename: O
authors.initials: O
authors.lastname: Holst
authors.forename: V
authors.initials: V
authors.lastname: Christoffel
authors.forename: R
authors.initials: R
authors.lastname: Fründ
authors.forename: H
authors.initials: H
authors.lastname: Moll
authors.forename: M
authors.initials: M
authors.lastname: Sumper
mesh_terms: D001089:Arabinose; D000460:Chlorophyta / Q000032:analysis*; D002849:Chromatography, Gas; D005109:Extracellular Matrix / Q000032:analysis; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D010446:Peptide Fragments / Q000302:isolation & purification; D010710:Phosphates / Q000378:metabolism; D010748:Phosphopeptides / Q000302:isolation & purification; D010761:Phosphorus Radioisotopes
chemical_list: D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D010446:Peptide Fragments; D010710:Phosphates; D010748:Phosphopeptides; D010761:Phosphorus Radioisotopes; C059656:sulfated glycoprotein 185; D001089:Arabinose