title: Introduction of a Micrococcus plasmid in Escherichia coli.
abstract: A 6-MDa plasmid (pMQV10), carrying cholesterol hydroxylase and streptomycin-resistance genes, from a gram-positive strain of Micrococcus sps., (RJ6) has been successfully transformed in gram-negative Escherichia coli K12 C600. pMQV10 is maintained stably and expresses its drug resistance in the new host.
authors.affiliation: Genetic Engineering Unit, Regional Research Laboratory, Jammu-Tawi, India.
authors.forename: V
authors.initials: V
authors.lastname: Verma
authors.forename: G N
authors.initials: GN
authors.lastname: Qazi
authors.forename: R
authors.initials: R
authors.lastname: Parshad
authors.forename: C L
authors.initials: CL
authors.lastname: Chopra
mesh_terms: D002790:Cholesterol 7-alpha-Hydroxylase / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D008837:Micrococcus / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D010957:Plasmids; D013307:Streptomycin / Q000494:pharmacology; D014169:Transformation, Bacterial
chemical_list: D002790:Cholesterol 7-alpha-Hydroxylase; D013307:Streptomycin