Platelet aggregating activity in human embryo culture media free of PAF-acether.
A factor activating human platelets and liberated by the embryo was sought in the culture media of human embryos using two bioassays. The first bioassay demonstrated the existence of a thrombocytopaenic factor after the i.p. injection of culture media into splenectomized mice. This factor was found more frequently in media which contained an embryo compared to those which contained a non-fertilized oocyte. PAF-acether (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) was searched for with a specific bioassay, using washed rabbit platelets. This remained negative for all the media studied, including those which had contained an embryo giving rise to a pregnancy. In these experiments it was not possible to relate embryo-derived platelet activating factor (EDPAF) to PAF-acether. Neither were we able to use the detection of EDPAF to test embryonic viability, or attempt to identify those embryos which were susceptible to lead to a pregnancy after intrauterine transfer from among all the embryos transferred.
Amiel
M L
ML
INSERM U187, Hôpital Antoine Béclère, Clamart, France.
Duquenne
C
C
Benveniste
J
J
Testart
J
J
D000818:Animals; D001681:Biological Assay; D002478:Cells, Cultured; D003470:Culture Media / Q000032:analysis; D004622:Embryo, Mammalian / Q000502:physiology*; D006801:Humans; D051379:Mice; D010972:Platelet Activating Factor / Q000032:analysis*; D010974:Platelet Aggregation; D013921:Thrombocytopenia / Q000139:chemically induced
D003470:Culture Media; D010972:Platelet Activating Factor