Interleukin 4 induces cellular adhesion among B lymphocytes.
We here report that interleukin 4 (IL-4) alone is able to induce cellular adhesion among mouse lymphocytes, and together with lipopolysaccharide (LPS), it increases the adhesion induced by LPS. The adhesion was inhibited by antibodies against IL-4. IL-4 appears to be acting mainly on B lymphocytes, since the response caused by IL-4 alone was much less sensitive to depletion of adherent cells than the LPS response. Depletion of T cells had no effect on IL-4- or LPS-induced adhesion. IL-4 could together with Con A, but not alone, induce adhesion among T cells. Cell clusters, which were formed after 2-3 days of LPS plus IL-4 stimulation, could be completely dissociated, and when the cells were recultured in medium, they readily started to reaggregate. The adhesion molecule lymphocyte function-associated antigen 1 (LFA-1) is, at least in part, involved in LPS plus IL-4-induced adhesion. Antibodies against LFA-1 inhibited the adhesion, but antibodies against other cell surface molecules were without inhibitory effect. Adhesion induced by IL-4 alone may involve other adhesion molecules than LFA-1.
Elenström
C
C
Department of Immunology, Arrhenius Laboratories for Natural Sciences, University of Stockholm, Sweden.
Severinson
E
E
D000818:Animals; D000943:Antigens, Differentiation / Q000037:antagonists & inhibitors / Q000502:physiology; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D002448:Cell Adhesion / Q000187:drug effects / Q000276:immunology; D002462:Cell Membrane / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects; D066298:In Vitro Techniques; D015847:Interleukin-4 / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D016169:Lymphocyte Function-Associated Antigen-1; D051379:Mice; D008815:Mice, Inbred Strains; D016028:Receptors, Leukocyte-Adhesion / Q000037:antagonists & inhibitors / Q000502:physiology
D000943:Antigens, Differentiation; D008070:Lipopolysaccharides; D016169:Lymphocyte Function-Associated Antigen-1; D016028:Receptors, Leukocyte-Adhesion; D015847:Interleukin-4