Analysis of monoclonal antibodies for identification of antigens associated with tumours of the gastrointestinal tract.
The reactivity of a new anti-human gastric carcinoma antibody, GL-013 with gastrointestinal tissue sections, with subcellular membranes (both from normal and malignant specimens), and with a variety of purified tumour antigens, has been evaluated and compared with that of a panel of monoclonal antibodies. These include antibodies against carcinoembryonic antigen (CEA), normal cross reacting antigen (NCA) and the Y-hapten. The reactivity of GL-013 antibody was distinct from, but not superior to that of the existing reagents. Of these, the antibody against the Y hapten was the most discriminatory with respect to its preferential reactivity with tumours, and the target tetrasaccharide comprising the Y hapten determinant was identified upon a variety of cellular macromolecules, including glycoproteins, glycolipids and on epidermal growth factor receptors. This is in accord with its frequent association with tumours.
Yang
G Y
GY
Cancer Research Campaign Laboratories, University of Nottingham, University Park, U.K.
Price
M R
MR
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D003106:Colon / Q000473:pathology; D003110:Colonic Neoplasms / Q000473:pathology*; D005753:Gastric Mucosa / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D007413:Intestinal Mucosa / Q000473:pathology; D009362:Neoplasm Metastasis; D012004:Rectal Neoplasms / Q000473:pathology*; D013270:Stomach / Q000473:pathology; D013274:Stomach Neoplasms / Q000473:pathology*
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor