Effects of 3-deazaadenosine, an inducer of HL-60 cell differentiation, on human blood cells in vitro.
The adenosine analogue 3-deazaadenosine (c3 Ado) induces differentiation of human promyelocytic leukemia HL-60 cells in vitro. c3 Ado affects in vitro viability and growth of cells involved indirectly (cytokine-producing cells) and directly (transformed myeloid cells) in leukemic cell differentiation. Growth inhibition of proliferating cells is achieved at c3 Ado concentrations between 10-100 microM. Concentrations higher than 100 microM affect viability of proliferating and nonproliferating cells.
Prytz
S
S
Department of Pharmacology, University of Tromsø, Norway.
Loennechen
T
T
Johansson
A
A
Larsen
L B
LB
Slørdal
L
L
Aarbakke
J
J
D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division; D002460:Cell Line; D003208:Concanavalin A / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000473:pathology; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects*; D014411:Neoplastic Stem Cells / Q000187:drug effects / Q000473:pathology; D013268:Stimulation, Chemical; D014372:Tubercidin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
C018258:3-deazaadenosine; D003208:Concanavalin A; D014372:Tubercidin