Covalent structure of collagen. Amino acid sequence of an arthritogenic cyanogen bromide peptide from type II collagen of bovine cartilage.
Bovine articular type II collagen was prepared by limited pepsin digestion, differential salt fractionation and carboxymethylcellulose chromatography. Cyanogen bromide digestion of purified type II collagen alpha chains yielded twelve distinct peptides designated CB1-12. The peptide alpha 1(II)-CB11 was isolated by carboxymethylcellulose chromatography and Sephadex G-75S gel filtration. Automated Edman degradation together with chymotrypsin, thermolysin and trypsin digestion enabled identification of its complete amino acid sequence. Compared with type I and type III collagen, the data show similarity with alpha 1(I)-CB8 and alpha 1(III)-CB6-1-8-10-2 peptides, respectively. The peptide is located within residues 124-402 of the alpha 1(II) collagen chain and with its identification, now extends the known amino acid sequence of bovine type II cartilage collagen to 660 amino acid residues including alpha 1(II)-CB1-2-6-12-11-8-10 (partial). This corresponds to alpha 1(I)-CB0-1-2-4-5-8-3-7 (partial; 1-660) and alpha 1(III)-CB3A-3B-3C-7-6-1-8-10-2-4-5 (partial; 1-660) of bovine alpha 1(I) and alpha 1(III) collagen chains.
Seyer
J M
JM
Connective Tissue Research Laboratory, Veterans Administration Medical Center, Memphis, TN 38104.
Hasty
K A
KA
Kang
A H
AH
D000595:Amino Acid Sequence; D000818:Animals; D001169:Arthritis, Experimental; D002358:Cartilage, Articular; D002417:Cattle; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D002918:Chymotrypsin; D003094:Collagen / Q000302:isolation & purification / Q000633:toxicity; D003488:Cyanogen Bromide; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification* / Q000633:toxicity; D013820:Thermolysin; D014357:Trypsin
D010446:Peptide Fragments; D003094:Collagen; D002918:Chymotrypsin; D014357:Trypsin; D013820:Thermolysin; D003488:Cyanogen Bromide