In vitro reconstitution of hnRNP particles.
The assembly of hnRNP-like particles was studied by in vitro reconstitution, UV-crosslinking and CsCl-equilibrium centrifugation. Using total nuclear protein and RNA extracts from HeLa cells for RNP reconstitution, RNP particles sedimenting with the same buoyant density of p = 1.4 g/cm3 as 'native' 40 S core hnRNPs were obtained. Under the stringent reconstitution conditions used, hnRNP complexes containing only the Cl-core hnRNP protein could be identified.
Schenkel
J
J
University of Heidelberg Medical School, FRG.
Kloetzel
P M
PM
D002467:Cell Nucleus / Q000032:analysis; D002499:Centrifugation, Density Gradient; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells / Q000032:analysis; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D006801:Humans; D012332:RNA, Heterogeneous Nuclear; D012261:Ribonucleoproteins / Q000302:isolation & purification; D014466:Ultraviolet Rays
D003432:Cross-Linking Reagents; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D012332:RNA, Heterogeneous Nuclear; D012261:Ribonucleoproteins