title: [Use of a gelatin substrate for culturing diploid human skin fibroblasts].
abstract: Gelatin coating of the growth surface of commercially available polystyrene Petri dishes is proposed for successful cultivation of human skin fibroblasts. A comparison of culture growth dynamics on gelatin, glass and polystyrene allowed to recommend gelatin as the substrate in order to receive an abundant similar material with a low population density viability. The absence of changes in carbohydrate metabolism changes during cell culturing on gelatin provides an opportunity to use these cells in studying regulation of carbohydrate metabolism.
authors.forename: K A
authors.initials: KA
authors.lastname: Nizheradze
authors.forename: N Iu
authors.initials: NIu
authors.lastname: Evdokimova
mesh_terms: D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media; D004171:Diploidy; D005347:Fibroblasts / Q000166:cytology; D005780:Gelatin; D005898:Glass; D006801:Humans; D011137:Polystyrenes; D012867:Skin / Q000166:cytology*; D013997:Time Factors
chemical_list: D003470:Culture Media; D011137:Polystyrenes; D005780:Gelatin
vernacular_title: Primenenie zhelatinovogo substrata dlia kul'tivirovaniia diploidnykh fibroblastov kozhi cheloveka.