Computer simulation and experimental validation of the electrophoretic behavior of proteins.
A mathematical model of the electrophoretic behavior of proteins is presented. The Debye-Hückel-Henry theory is used for the description of protein mobility, which has the important result of making net mobility a function of ionic strength. A net charge vs pH relationship and a diffusion coefficient are required to describe a specific protein. The model is employed for the computer simulation of three distinct electrophoretic modes: isoelectric focusing, isotachophoresis, and zone electrophoresis. The validity of the model is tested by comparing simulation with experimental data. Excellent qualitative agreement was found.
Mosher
R A
RA
Dewey
D
D
Thormann
W
W
Saville
D A
DA
Bier
M
M
D003198:Computer Simulation; D004586:Electrophoresis; D006863:Hydrogen-Ion Concentration; D008433:Mathematics; D011506:Proteins / Q000032:analysis*
D011506:Proteins