Characterization of the selective inhibition of growth of virulent Legionella pneumophila by supplemented Mueller-Hinton medium.
The phenotypic difference between virulent and avirulent Legionella pneumophila with regard to growth on supplemented Mueller-Hinton (SMH) agar was investigated. Defined populations of virulent and avirulent L. pneumophila were inoculated onto hybrid growth media containing the combination of SMH agar components with potassium phosphate-buffered charcoal-yeast extract agar. The casein acid hydrolysate component of SMH agar was found to be inhibitory to the growth of virulent but not avirulent cells. The selectively inhibitory component of the casein acid hydrolysate was identified as NaCl.
Catrenich
C E
CE
Department of Microbiology, University of Iowa, Iowa City 52242.
Johnson
W
W
D000362:Agar; D002364:Caseins; D002606:Charcoal; D003470:Culture Media / Q000494:pharmacology*; D006131:Growth Inhibitors / Q000494:pharmacology*; D007875:Legionella / Q000187:drug effects / Q000254:growth & development* / Q000472:pathogenicity; D010710:Phosphates; D011188:Potassium; D017680:Potassium Compounds; D011492:Protein Hydrolysates; D012965:Sodium Chloride / Q000502:physiology; D014774:Virulence / Q000187:drug effects
D002364:Caseins; D003470:Culture Media; D006131:Growth Inhibitors; D010710:Phosphates; D017680:Potassium Compounds; D011492:Protein Hydrolysates; C026375:trypticase-soy broth; D002606:Charcoal; D012965:Sodium Chloride; D000362:Agar; C013216:potassium phosphate; D011188:Potassium