Photolytic interface for high-performance liquid chromatography--chemiluminescence detection of non-volatile N-nitroso compounds.
A photolytic interface between high-performance liquid chromatography (HPLC) and a chemiluminescence detector has been developed for the trace detection of non-volatile N-nitroso compounds in biological matrices. A chromatographic effluent containing separated N-nitrosoamino acids and N-nitrosamides is introduced into a glass coil with a purge stream of He and irradiated with ultraviolet light. Nitrogen oxide, cleaved by photolysis, is separated rapidly from the solvent through a series of cold traps and carried by the He into the reaction chamber of a chemiluminescence detector. The method is compatible with most types of HPLC, especially reversed-phase, and yields low-nanogram sensitivity for underivatised N-nitrosoamino acids and N-nitrosamides. The detection of a model N-nitrosamide, trimethylnitrosourea, in spiked porcine gastric fluid (42 micrograms l-1), and of N-nitrosoproline and N-nitroso-1,3-thiazolidine-4-carboxylic acid, in spiked human urine (7-8 micrograms l-1), is demonstrated.
Conboy
J J
JJ
Hotchkiss
J H
JH
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D005750:Gastric Juice / Q000032:analysis; D006801:Humans; D049449:Luminescence; D009603:Nitroso Compounds / Q000032:analysis / Q000652:urine*; D013552:Swine
D009603:Nitroso Compounds