In vitro growth of rat bone marrow BFU-E.
Pokeweed mitogen-stimulated rat spleen cells were identified as a reliable source of rat burst-promoting activity (PBA), which permitted development of a reproducible assay for rat bone marrow erythroid burst-forming units (BFU-E). Optimum BPA dose, assay time, cell dose, and erythropoietin requirements for rat BFU-E were identified. A serum-free assay and a method for stimulating endogenous bone marrow BPA were developed. Identification of sources of rat BPA and characterization of the rat BFU-E assay makes this species more useful for hematopoietic studies. The similarity of rat BFU-E to human and mouse BFU-E strengthens the validity of rat models for erythropoiesis.
Meagher
R C
RC
Department of Laboratory Hematology, Cleveland Clinic Foundation, Ohio.
Rothmann
S A
SA
Paik
I K
IK
Paul
P
P
D000818:Animals; D001790:Blood Physiological Phenomena; D001853:Bone Marrow; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003470:Culture Media; D004900:Erythroblasts / Q000187:drug effects / Q000502:physiology*; D004920:Erythropoiesis / Q000187:drug effects; D004921:Erythropoietin / Q000494:pharmacology; D005260:Female; D006801:Humans; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen
D003470:Culture Media; D004921:Erythropoietin