Rapid diagnosis of Rift Valley fever: a comparison of methods for the direct detection of viral antigen in human sera.
Human sera collected during the 1987 Rift Valley fever (RVF) epidemic in the Senegal River basin were analysed using three enzyme immunoassays to establish the best method for rapid diagnosis of RVF. A biotin-avidin-enhanced antigen detection method utilizing monoclonal antibodies proved most sensitive. Eighty-two viremic human sera were tested, and this assay detected antigen in 29.3% of the samples.
Meegan
J
J
U.S. Army Medical Research Institute of Infectious Diseases, Frederick, MD 21701.
Le Guenno
B
B
Ksiazek
T
T
Jouan
A
A
Knauert
F
F
Digoutte
J P
JP
Peters
C J
CJ
D000354:Africa, Western; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000032:analysis*; D001360:Avidin; D001710:Biotin; D002043:Bunyaviridae / Q000276:immunology*; D004196:Disease Outbreaks; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D011237:Predictive Value of Tests; D012295:Rift Valley Fever / Q000175:diagnosis* / Q000453:epidemiology; D012296:Rift Valley fever virus / Q000276:immunology* / Q000302:isolation & purification; D013997:Time Factors
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007106:Immune Sera; D007075:Immunoglobulin M; D001360:Avidin; D001710:Biotin