title: A calcium-dependent antibody for identification and purification of recombinant proteins.
abstract: We report a straightforward methodology for purification of recombinant proteins by incorporating a short hydrophilic peptide marker segment at their N-termini. A calcium-dependent antibody that reacts primarily with the first three amino acids of this peptide segment was used to affinity purify the fusion proteins in a single chromatographic step. The marker peptide could subsequently be removed by proteolysis with the enzyme enterokinase.
authors.affiliation: Immunex Corporation, Seattle, WA 98101.
authors.forename: K S
authors.initials: KS
authors.lastname: Prickett
authors.forename: D C
authors.initials: DC
authors.lastname: Amberg
authors.forename: T P
authors.initials: TP
authors.lastname: Hopp
mesh_terms: D000911:Antibodies, Monoclonal; D002118:Calcium; D002846:Chromatography, Affinity / Q000379:methods*; D004765:Enteropeptidase; D005822:Genetic Vectors; D007377:Interleukin-3 / Q000235:genetics / Q000302:isolation & purification*; D015847:Interleukin-4 / Q000235:genetics / Q000302:isolation & purification*; D010957:Plasmids; D011994:Recombinant Proteins / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae
chemical_list: D000911:Antibodies, Monoclonal; D007377:Interleukin-3; D011994:Recombinant Proteins; D015847:Interleukin-4; D004765:Enteropeptidase; D002118:Calcium