A BASIC program for the estimation of Michaelis-Menten parameters by the direct linear plot.
The use of manual graphical methods for the estimation of Michaelis-Menten kinetic parameters as recommended by Eisenthal and Cornish-Bowden (Biochem. J. 139 (1974) 715-720) was found to be impractical for large (n greater than 5) sample numbers. A BASIC program providing the rank ordered coordinates of intersections, which correspond to estimates of Km and Vmax in the direct linear plot method, is described.
Brady
J F
JF
Department of Chemical Biology and Pharmacognosy, College of Pharmacy, Rutgers University, Piscataway, NJ 08855.
Ishizaki
H
H
D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D003640:Dealkylation; D004798:Enzymes / Q000378:metabolism*; D007700:Kinetics; D008432:Mathematical Computing; D008838:Microcomputers; D008956:Models, Chemical; D011381:Programming Languages; D012984:Software
D004798:Enzymes; D003577:Cytochrome P-450 Enzyme System