title: Binding of glycyrrhizin to human serum and human serum albumin.
abstract: The binding of glycyrrhizin (GLZ) to human serum and human serum albumin (HSA) was examined by an ultrafiltration technique. Specific and nonspecific bindings were observed in both human serum and HSA. The association constants (K) for the specific bindings were very similar: 1.31 x 10(5) M-1 in human serum and 3.87 x 10(5) M-1 in HSA. The number of binding sites (n) and the linear binding coefficient (phi) in HSA were 1.95 and 3.09 x 10(3) M-1, respectively. When the human serum protein concentration was assumed to be 4.2% (equal to the measured serum albumin concentration), n in human serum was 3.09, which is similar to the n value in HSA, and phi in human serum was 0.71 x 10(3) M-1, which is reasonably close to that for HSA. The binding pattern of GLZ with human serum protein on Sephadex G-200 column chromatography showed that GLZ binds to only the albumin fraction. It was concluded that the GLZ-binding sites in human serum exist mainly on albumin and GLZ binds to specific and nonspecific binding sites at lower and higher concentrations than approximately 2 mM, respectively.
authors.forename: S
authors.initials: S
authors.lastname: Ishida
authors.forename: Y
authors.initials: Y
authors.lastname: Sakiya
authors.forename: T
authors.initials: T
authors.lastname: Ichikawa
authors.forename: M
authors.initials: M
authors.lastname: Kinoshita
authors.forename: S
authors.initials: S
authors.lastname: Awazu
mesh_terms: D006034:Glycyrrhetinic Acid / Q000031:analogs & derivatives* / Q000097:blood; D019695:Glycyrrhizic Acid; D006801:Humans; D011485:Protein Binding; D012709:Serum Albumin
chemical_list: D012709:Serum Albumin; D019695:Glycyrrhizic Acid; D006034:Glycyrrhetinic Acid