ras p21 expression in ovine pulmonary carcinoma.
We have adapted an enzyme-linked immunoblot assay (ELIBA) for the detection of a c-ras proto-oncogene and oncogene protein products in human cell lines and tumors of 21,000 daltons molecular weight (p21ras) to studies of tissues derived from sheep. In the ELIBA, a double antibody system is used in which p21ras proteins are initially immunoprecipitated from protein extracts with monoclonal antibodies, and subsequently identified using additional anti-ras antibodies. Binding is identified with a non-radioactive enzyme-linked colorimetric detection system. In the present study, the ELIBA system was used to study twenty-seven ovine lung specimens, representing normal lung, inflammatory, and neoplastic lesions. We detected p21ras protein expression in every tissue examined, but the nature and amount of the protein product varied significantly among the tissues examined. Some tissues expressed multiple ras species. Broncho-alveolar carcinoma specimens were most likely to express c-Ki-ras proteins. Mutant proteins of c-N-ras and c-Ki-ras were detected in several bronchoalveolar carcinoma specimens, based on migrational differences between mutant and normal proteins in 15% polyacrylamide gels. The results of this study demonstrate the utility of the ELIBA system for detection of c-ras expression in ovine lung tissues, and demonstrate the ability of the system to discriminate specific ras protein species. The prognostic significance of ras expression in sheep pulmonary carcinoma has yet to be determined.
Meyers
F J
FJ
Department of Internal Medicine, University of California, Davis Medical Center, Sacramento 95817.
Madewell
B R
BR
Gumerlock
P H
PH
DeMartini
J C
JC
D000818:Animals; D015972:Gene Expression Regulation, Neoplastic; D011905:Genes, ras; D015151:Immunoblotting / Q000379:methods; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism / Q000662:veterinary*; D015689:Oncogene Protein p21(ras) / Q000235:genetics* / Q000378:metabolism; D000090063:Proto-Oncogene Mas; D012756:Sheep; D012757:Sheep Diseases / Q000235:genetics* / Q000378:metabolism
C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D015689:Oncogene Protein p21(ras)