The state of DNA methylation of human epsilon-globin gene in erythroid and non-erythroid cells.
The extent of DNA methylation within the embryonic human epsilon-globin gene domain was studied in erythroid and non-erythroid cell lines. The results obtained show that the human epsilon-globin gene is totally methylated at all sites tested in tissues where it is not expressed, i.e. blood leucocytes. In the erythroid cell lines, K562 and PUTKO, both forced to embryonic differentiation by induction with haemin, the level of methylation is reduced compared with that observed in blood leucocytes. In the nonerythroid cell lines HeLa and Rajii, where the human epsilon-globin gene is not expressed, the overall level of methylation in all sites tested is lower compared with that in erythroid cell lines.
Bartzeliotou
A J
AJ
National Hellenic Research Foundation, Athens, Greece.
Dimitriadis
G J
GJ
D002460:Cell Line; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis*; D005914:Globins / Q000032:analysis / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D007962:Leukocytes / Q000032:analysis*; D008745:Methylation
D005914:Globins; D004247:DNA