Labeling of Salmonella typhimurium with iodine-131 to study phagocytic function in rats.
The present study describes a method for labeling Salmonella typhymurium with iodine-131 to evaluate both the morphological and the functional characteristics of the reticulo-endothelial system. A suspension containing 2 x 10(9) bacteria per ml was labeled with carrier-free Na131I without reductor, with a labeling yield of 46.5 +/- 3% and 3.5 +/- 1.3% of free Iodine-131. The biodistribution of the labeled bacteria in rats was studied with a large-field-of-view scintillation camera equiped with a pinhole collimator. Whole body images were obtained 15 and 30 minutes after intravenous injection of the labeled microorganisms. Images showed accumulation of bacteria in the liver and both normal and transplanted spleens of the animals. Autoradiographs of liver and spleen demonstrated labeled bacteria within the cells of the reticulo-endothelial system. The method described is easy to perform, has a good labeling yield and allows the functional evaluation of the reticulo-monophagocytic system, including transplanted spleens.
Sato
M K
MK
Rodrigues Júnior
A J
AJ
Camargo
E E
EE
D000818:Animals; D001345:Autoradiography; D007457:Iodine Radioisotopes; D008099:Liver / Q000000981:diagnostic imaging; D012157:Mononuclear Phagocyte System / Q000502:physiology*; D010587:Phagocytosis; D011859:Radiography; D051381:Rats; D011919:Rats, Inbred Strains; D012486:Salmonella typhimurium; D013154:Spleen / Q000000981:diagnostic imaging
D007457:Iodine Radioisotopes