title: High voltage electron microscopic studies of endothelial cell tubular structures in the mouse blood-brain barrier following brain trauma.
abstract: High-voltage electron microscopy was applied to the study of endothelial cell (EC) transport of macromolecules in a murine model of blood-brain barrier injury to study the role of the EC canalicular system following brain insult. Semithick sections from mouse brains subjected to acute (2-3 h) mechanical trauma demonstrated permeation of intravenously injected horseradish peroxidase via tubular structures either (a) in the absence of lysosome-associated structures in close proximity, or (b) in association with lysosomes, dense bodies or multivesicular bodies. Our data suggest a dual-purposed system of tubules, one portion that supplies the metabolic requirements of the cell and another portion, suggested to be more limited, that opens up as a result of brain injury.
authors.affiliation: NYS Office of Mental Retardation and Developmental Disabilities, Staten Island 10314.
authors.forename: A S
authors.initials: AS
authors.lastname: Lossinsky
authors.forename: M J
authors.initials: MJ
authors.lastname: Song
authors.forename: H M
authors.initials: HM
authors.lastname: Wisniewski
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier; D001930:Brain Injuries / Q000473:pathology*; D004730:Endothelium, Vascular / Q000503:physiopathology / Q000648:ultrastructure*; D005260:Female; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron