title: Enantioselective gas chromatographic assays with electron-capture detection for methoxyphenamine and its three primary metabolites in human urine.
abstract: Sensitive and enantioselective gas chromatographic assays have been developed and applied to the quantitation in human urine of the enantiomers of methoxyphenamine and its three primary oxidative metabolites, namely, N-desmethylmethoxyphenamine, O-desmethylmethoxyphenamine and 5-hydroxymethoxyphenamine. The separation of the various analytes was achieved through the combined use of high-resolution gas chromatography coupled with electron-capture detection and employing a capillary OV-225 column. The formation of diastereometric derivatives involved the chiral acylating reagent N-heptafluorobutyryl-L-prolyl chloride. The assays for methoxyphenamine and O-desmethylmethoxyphenamine were linear over the range 0.25-2.0 micrograms/ml for each analytes' enantiomers, while in the case of the enantiomers for N-desmethylmethoxyphenamine and 5-hydroxymethoxyphenamine linearity was shown over the ranges 0.094-0.75 and 0.188-1.5 micrograms/ml, respectively. The mean coefficients of variation in all cases were less than 4%.
authors.affiliation: College of Pharmacy, University of Saskatchewan, Saskatoon, Canada.
authors.forename: N R
authors.initials: NR
authors.lastname: Srinivas
authors.forename: J W
authors.initials: JW
authors.lastname: Hubbard
authors.forename: E M
authors.initials: EM
authors.lastname: Hawes
authors.forename: G
authors.initials: G
authors.lastname: McKay
authors.forename: K K
authors.initials: KK
authors.lastname: Midha
mesh_terms: D000662:Amphetamines / Q000652:urine; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D006801:Humans; D008694:Methamphetamine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000652:urine; D013237:Stereoisomerism
chemical_list: D000662:Amphetamines; C043935:N-desmethylmethoxyphenamine; D008694:Methamphetamine; C043936:O-desmethylmethoxyphenamine; C043934:5-hydroxymethoxyphenamine; C005157:methoxyphenamine