[Direct gas chromatography analysis of volatile fatty acids and lactic acids in microbiological diagnosis].
The gas chromatographic analysis of short-chain fatty acids is of great significance in the rapid diagnosis of anaerobic infections and bacterial meningitis. A new analytical method is presented detecting lactic acid without prior derivatisation in addition to volatile fatty acids in the aqueous phase directly. Lactic acid levels greater than 30 mg/dl are measured in cerebrospinal fluids of patients with acute bacterial meningitis. The sole detection of acetic acid in concentrations higher than 20 mg/dl indicates aerobes in clinical materials. A high correlation exists between the determination of n-butyric acid as well as iso-valeric acid and the occurrence of anaerobic bacteria.
Socolowsky
S
S
Höhne
C
C
Sandow
D
D
D001421:Bacteria, Anaerobic / Q000302:isolation & purification; D001424:Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D001431:Bacteriological Techniques; D002849:Chromatography, Gas; D005232:Fatty Acids, Volatile / Q000032:analysis*; D006801:Humans; D007773:Lactates / Q000032:analysis*; D008581:Meningitis / Q000175:diagnosis* / Q000382:microbiology
D005232:Fatty Acids, Volatile; D007773:Lactates
Zur gaschromatographischen Direktanalyse von flüchtigen Fettsäuren und Milchsäure in der mikrobiologischen Diagnostik.