cDNA structure and expression of bombyxin, an insulin-like brain secretory peptide of the silkmoth Bombyx mori.
Bombyxin, previously referred to as 4K-prothoracicotropic hormone, is a brain peptide of the silkmoth Bombyx mori, the amino acid sequence of which shows considerable homology with vertebrate insulin family peptides. Two independent clones have been isolated from a Bombyx larval brain cDNA library by using a synthetic oligonucleotide probe, one with the complete coding region for preprobombyxin (lambda Bb360) and the other covering the coding region, possibly for bombyxin, only partially (lambda Bb204). lambda Bb360 encodes preprobombyxin in the order of prepeptide/B-chain/proteolytic cleavage signal/C-peptide/proteolytic cleavage signal/A-chain. This domain organization of preprobombyxin is the same as that of preproinsulins, suggesting that the tertiary structure and posttranslational modification mechanism are conserved through the evolution of bombyxin and insulin. Genomic Southern hybridization analyses using this cDNA as probe suggest that the Bombyx genome contains multiple copies of bombyxin gene. Northern hybridization analyses indicate that the concentration of lambda Bb360-type bombyxin mRNA in the bombyxin-producing cells is remarkably high (2.8 x 10(9) molecules/micrograms of total RNA), without undergoing appreciable change during larval-pupal development.
Adachi
T
T
National Institute for Basic Biology, Okazaki, Japan.
Takiya
S
S
Suzuki
Y
Y
Iwami
M
M
Kawakami
A
A
Takahashi
S Y
SY
Ishizaki
H
H
Nagasawa
H
H
Suzuki
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D012831:Bombyx / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D007301:Insect Hormones / Q000235:genetics*; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics*; D015345:Oligonucleotide Probes; D011384:Proinsulin / Q000235:genetics; D011498:Protein Precursors / Q000235:genetics
D007301:Insect Hormones; D009479:Neuropeptides; D015345:Oligonucleotide Probes; D011498:Protein Precursors; C009089:prothoracicotropic hormone; D004247:DNA; D011384:Proinsulin