title: Development and characterization of monoclonal antibodies reactive with paraquat.
abstract: A set of three anti-paraquat monoclonal antibodies(MoAbs), named APM-1, APM-2 and APM-3, has been isolated. In order to evaluate the ability of these MoAbs to recognize various kinds of bipyridyl herbicides and similar congeners of paraquat, a competition enzyme-linked immunosorbent assay (ELISA) using avidin-biotin complex (ABC) was developed. All three antibodies strongly recognized paraquat and slightly did the other analogs. These three MoAbs are therefore advantageous to a toxicological study of paraquat and of its localization in tissues.
authors.affiliation: Department of Legal Medicine, Hokkaido University School of Medicine, Sapporo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Nagao
authors.forename: T
authors.initials: T
authors.lastname: Takatori
authors.forename: B
authors.initials: B
authors.lastname: Wu
authors.forename: K
authors.initials: K
authors.lastname: Terazawa
authors.forename: H
authors.initials: H
authors.lastname: Gotouda
authors.forename: H
authors.initials: H
authors.lastname: Akabane
mesh_terms: D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001360:Avidin; D001710:Biotin; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006241:Haptens; D006825:Hybridomas / Q000276:immunology; D010269:Paraquat / Q000031:analogs & derivatives / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D006241:Haptens; D001360:Avidin; D001710:Biotin; D010269:Paraquat