title: Tacaribe virus L gene encodes a protein of 2210 amino acid residues.
abstract: The nucleotide sequence of Tacaribe virus (TV) L gene was obtained from two sets of overlapping cDNA clones constructed by walking along the virus L RNA using two successive synthetic DNA primers. Analysis of the sequence indicated the existence of a unique long open reading frame in the viral complementary strand. The first in-phase AUG codon is in positions 31-33 from the 5' end of the viral complementary L RNA surrounded by a sequence favorable for initiation of protein synthesis. The open reading frame ends at positions 6661-6663. The predicted TV L protein is a 2210 amino acid long polypeptide with an estimated molecular weight of 251,942. Comparison of the amino acid sequence of TV L protein with peptide sequences predicted from L-derived cDNA clones of lymphocytic choriomeningitis virus shows an overall 42% of homology.
authors.affiliation: Centro de Virologia Animal, Buenos Aires, Argentina.
authors.forename: S
authors.initials: S
authors.lastname: Iapalucci
authors.forename: R
authors.initials: R
authors.lastname: Lopez
authors.forename: O
authors.initials: O
authors.lastname: Rey
authors.forename: N
authors.initials: N
authors.lastname: Lopez
authors.forename: M T
authors.initials: MT
authors.lastname: Franze-Fernandez
authors.forename: G N
authors.initials: GN
authors.lastname: Cohen
authors.forename: M
authors.initials: M
authors.lastname: Lucero
authors.forename: A
authors.initials: A
authors.lastname: Ochoa
authors.forename: M M
authors.initials: MM
authors.lastname: Zakin
mesh_terms: D000595:Amino Acid Sequence; D001116:Arenaviridae / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D008217:Lymphocytic choriomeningitis virus / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009975:Orthomyxoviridae / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins