Sorbitol metabolism in retina studied in vitro.
A new method is described which allows study of the retinal sorbitol metabolism in vitro. Bovine retinal tissue was incubated in tissue culture medium for 24 h and after this time showed only small morphological changes. The sorbitol content doubled at a glucose level of 5.5 mM compared with freshly prepared noncultured retina. Increasing glucose concentrations led to a gradual increase of the sorbitol content. Supplementation of the culture medium with fructose likewise enhanced the retinal sorbitol accumulation. An aldose reductase inhibitor (ICI 128436; Statil) significantly decreased the sorbitol content.
Naeser
P
P
Department of Ophthalmology, Uppsala University, Sweden.
Brolin
S E
SE
Eriksson
U J
UJ
D000818:Animals; D002417:Cattle; D003470:Culture Media; D005632:Fructose; D005947:Glucose; D066298:In Vitro Techniques; D012160:Retina / Q000378:metabolism*; D013012:Sorbitol / Q000378:metabolism*; D013997:Time Factors
D003470:Culture Media; D005632:Fructose; D013012:Sorbitol; D005947:Glucose