title: Antispermatogenic effect of embelin, a plant benzoquinone, on male albino rats in vivo and in vitro.
abstract: Embelin, the active principle of the seeds of Embelia ribes Burm, has been isolated and the purity established. Daily subcutaneous administration of the compound at a dose of 20 mg/kg body weight to male albino rats for 15 or 30 days revealed an inhibition of: a) epididymal motile sperm count, b) fertility parameters such as pregnancy attainment and litter size, and c) the activities of the enzymes of glycolysis and energy metabolism. These changes were reversible, as seen after 15 and 30 days of recovery. Addition of embelin to epididymal sperm suspensions caused a dose- and duration-dependent inhibition of spermatozoal motility and the activities of the enzymes of carbohydrate metabolism. Light and scanning electron microscopy showed that both in vivo and in vitro treatment with the drug causes profound morphological changes in spermatozoa such as: a) decapitation of the spermatozoal head, b) discontinuity of the outer membranous sheath in the mid-piece and the tail region, and c) alteration in the shape of the cytoplasmic droplet in the tail.
authors.affiliation: Department of Zoology, Panjab University, Chandigarh, India.
authors.forename: S
authors.initials: S
authors.lastname: Gupta
authors.forename: S N
authors.initials: SN
authors.lastname: Sanyal
authors.forename: U
authors.initials: U
authors.lastname: Kanwar
mesh_terms: D000818:Animals; D016227:Benzoquinones; D050260:Carbohydrate Metabolism; D055598:Chemical Phenomena; D002621:Chemistry; D066298:In Vitro Techniques; D008297:Male; D011809:Quinones / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013076:Sperm Count / Q000187:drug effects; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000648:ultrastructure; D013997:Time Factors
chemical_list: D016227:Benzoquinones; D011809:Quinones; C010945:embelin