title: Failure of spermatozoa from T/t mice to fertilize in vitro is overcome by zona drilling.
abstract: Failure of epididymal spermatozoa from T/t mutant mice, but not from t/t individuals, to fertilize oocytes in vitro was partially overcome by opening a small aperture in the zona pellucida with acidified Tyrode's solution to permit direct access of the spermatozoon to the vitellus. This study provides a model system to evaluate requirements for successful zona drilling in the treatment of human infertility and further insights into the effects of the t complex on sperm fertility.
authors.affiliation: Research Institute and Gynecology, Cleveland Clinic Foundation, Ohio 44195-5240.
authors.forename: T
authors.initials: T
authors.lastname: Ahmad
authors.forename: J C
authors.initials: JC
authors.lastname: Conover
authors.forename: M M
authors.initials: MM
authors.lastname: Quigley
authors.forename: R L
authors.initials: RL
authors.lastname: Collins
authors.forename: A J
authors.initials: AJ
authors.lastname: Thomas
authors.forename: R B
authors.initials: RB
authors.lastname: Gwatkin
mesh_terms: D000818:Animals; D005260:Female; D005307:Fertilization in Vitro; D006579:Heterozygote; D007248:Infertility, Male / Q000503:physiopathology*; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D010063:Ovum / Q000502:physiology*; D051381:Rats; D013081:Sperm Motility; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000502:physiology*; D015044:Zona Pellucida / Q000502:physiology*