Expression of enzymatically active enkephalinase (neutral endopeptidase) in mammalian cells.
A cDNA encoding the rat enkephalinase protein (neutral endopeptidase; EC 3.4.24.11) has been constructed from overlapping lambda gt10 cDNA clones. This cDNA was inserted into an expression plasmid containing the cytomegalovirus enhancer and promoter. When transfected with this plasmid, Cos 7 cells transiently expressed the enkephalinase protein in a membrane-bound state. Recombinant enkephalinase recovered in solubilized extracts from transfected Cos 7 cells was enzymatically active and displayed properties similar to those of the native enzyme with respect to sensitivity to classical enkephalinase inhibitors.
Gorman
C M
CM
Department of Cell Genetics, Genentech, Inc., South San-Francisco, California 94080.
Gies
D
D
Schofield
P R
PR
Kado-Fong
H
H
Malfroy
B
B
D000818:Animals; D015153:Blotting, Western; D004791:Enzyme Inhibitors; D005786:Gene Expression Regulation; D015260:Neprilysin / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics*; D010957:Plasmids; D051381:Rats; D011994:Recombinant Proteins / Q000235:genetics
D004791:Enzyme Inhibitors; D011994:Recombinant Proteins; D015260:Neprilysin