title: Characterization of purinoceptors present on human liver plasma membranes.
abstract: Using ATP alpha 35S as radioligand, we have detected the presence of specific purinoceptors on human liver plasma membranes. They are characterized by a Kd value of 0.19 microM and a Bmax of 24 pmol/mg membrane protein. These purinoceptors belong to the P2Y subclass as demonstrated by the high degree of similarity with rat liver purinoceptors, previously shown to be P2Y [(1986) Biochem. J. 240, 367-371] and known to be involved in the control of liver glycogenolysis.
authors.affiliation: Afdeling Biochemie, Katholieke Universiteit Leuven, Belgium.
authors.forename: S
authors.initials: S
authors.lastname: Keppens
authors.forename: A
authors.initials: A
authors.lastname: Vandekerckhove
authors.forename: H
authors.initials: H
authors.lastname: De Wulf
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000032:analysis*; D006801:Humans; D008099:Liver / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D011983:Receptors, Purinergic / Q000145:classification / Q000302:isolation & purification* / Q000502:physiology; D013045:Species Specificity
chemical_list: D011983:Receptors, Purinergic; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate