Platelet thermophysiology: a new field of investigation dependent upon an improved sub-ambient platelet aggregometer.
Studies of platelet aggregation are normally performed at 37 degrees C. However, in hypothermia during certain surgical procedures (for example, cardiopulmonary bypass) efficient aggregation may be needed at temperatures below 37 degrees C: therefore, assessment of platelet aggregation at temperatures below 37 degrees C may be relevant. This paper describes a sub-ambient aggregometry system developed for this purpose. The effect of temperature (range of 4.5 degrees-37.0 degrees C) upon ADP-induced and spontaneous aggregation of platelets from normal subjects was studied. Platelet aggregation was found to be maximal at room temperature, and may represent 'pure' aggregation. At higher temperatures platelet disaggregation becomes measurable, and at lower temperatures, cold-induced platelet shape-change predominates. These parameters are briefly discussed in terms of their relevance to future research into the physiology of platelet storage and clinical haemostasis.
Trenchard
P M
PM
Welsh Regional Transfusion Centre, Rhydlafar, Cardiff, UK.
Jeffery
D M
DM
D000244:Adenosine Diphosphate / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007036:Hypothermia, Induced; D008722:Methods; D010974:Platelet Aggregation; D013696:Temperature
D000244:Adenosine Diphosphate