The 5'-region of the P450XIA1 (P450scc) gene contains a basal promoter and an adrenal-specific activating domain.
The first step to the synthesis of all steroids is catalyzed by P450scc. We constructed nine deletion mutants of the 5'-region of the P450scc gene and connected them to a CAT reporter gene to assay transcriptional activity of the P450scc promoter. A short 145 bp fragment stimulated transcription by two fold. This DNA was active in all cells tested irrespective of their tissue origin and steroidogenic activity. DNA at -145/-573 of the upstream region did not increase transcription any further. DNA including 2500 bp of the upstream region stimulated transcription by 10 fold only in adrenal Y-1 cells. Hence in the -145 region contains a low level P450scc promoter and the 2500 bp DNA possesses an adrenal specific enhancing element.
Chung
B C
BC
Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, Republic of China.
Hu
M C
MC
Lai
C C
CC
Lin
C H
CH
D000311:Adrenal Glands / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000235:genetics*; D015342:DNA Probes; D005260:Female; D006801:Humans; D009154:Mutation; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection
D015342:DNA Probes; D012333:RNA, Messenger; D002786:Cholesterol Side-Chain Cleavage Enzyme; D015500:Chloramphenicol O-Acetyltransferase