Subcellular fractionation of tissue culture cells.
Subcellular fractionation has two major steps, (1) the homogenization of the cells and (2) the subsequent separation of the organelles. The homogenization step is discussed with reference to the problems encountered using tissue culture cells. Promising techniques for the isolation of specific compartments are illustrated using the isolation of the endosomal compartment as the example.
Howell
K E
KE
Devaney
E
E
Gruenberg
J
J
D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D002478:Cells, Cultured; D046508:Culture Techniques; D013347:Subcellular Fractions