SS-B (La) nuclear antigen: fast and non-degradative procedure to prepare SS-B extracts free from other nuclear antigens.
A procedure is described allowing the easy and fast obtention of a cellular extract from calf thymus, enriched in the undegraded 52 kDa SS-B protein. As seen by western blot, the extract does not contain Sm and RNP antigens, allowing the use of such fraction for the detection of anti-SS-B antibodies without the interference of the anti-Sm and anti-RNP specificities. The enrichment avoids denaturing agents, making the fraction suitable for use in functional and structural studies.
Bach
M
M
Institut de Biologia de Barcelona, CSIC, Spain.
Palau
J
J
D000818:Animals; D001324:Autoantigens / Q000302:isolation & purification*; D015153:Blotting, Western; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D008722:Methods; D011480:Protease Inhibitors / Q000494:pharmacology; D012261:Ribonucleoproteins; D012967:Sodium Dodecyl Sulfate; D013950:Thymus Gland / Q000032:analysis; D000097585:SS-B Antigen
D001324:Autoantigens; D011480:Protease Inhibitors; D012261:Ribonucleoproteins; D012967:Sodium Dodecyl Sulfate; D000097585:SS-B Antigen