title: Glomerular adaptation in uninephrectomized young rats.
abstract: To study the glomerular adaptation during compensatory renal growth starting in infancy, we assessed afferent effective ultrafiltration pressure (PUF), glomerular filtration area and hydraulic conductivity in rats uninephrectomized (Nx) or sham-operated (S) at 5 days of age. Rats were fed a normal protein diet and studied at 20 and 60 days of age. Single nephron glomerular filtration rate was significantly higher in Nx than in S rats both at 20 days of age (mean +/- SEM: 15.0 +/- 1.5 vs 7.4 +/- 0.7 nl/min) and 60 days of age (80.7 +/- 4.6 vs 43.5 +/- 3.2 nl/min). Afferent effective PUF, estimated by the stop-flow method, was significantly higher in Nx than in S rats both at 20 days (22.5 +/- 0.8 vs 18.3 +/- 0.4 mmHg) and 60 days (28.3 +/- 1.0 vs 23.2 +/- 1.1 mmHg). The filtering area per glomerulus, calculated as the area of the glomerular basement membrane facing both the endothelial and the epithelial cells, but not the mesangial cells, was not different in Nx and in S rats at 20 days (3.0 +/- 0.3 vs 2.8 +/- 0.1 10(4) microns 2), but it was significantly greater in Nx than in S rats at 60 days (23.3 +/- 3.7 vs 9.9 +/- 0.9 10(4) microns 2). The hydraulic conductivity determined in isolated glomeruli was similar in Nx and in S rats at 20 days of age (1.40 +/- 0.11 vs 1.69 +/- 0.23 microliters/min.mmHg.cm2) but was significantly decreased in 60-day-old Nx rats, compared with S rats of the same age (1.52 +/- 0.11 vs 2.35 +/- 0.17 microliters/min.mmHg.cm2).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Pediatrics, St. Göran's Children's Hospital, Karolinska Institute, Stockholm, Sweden.
authors.forename: G
authors.initials: G
authors.lastname: Celsi
authors.forename: L
authors.initials: L
authors.lastname: Larsson
authors.forename: I
authors.initials: I
authors.lastname: Seri
authors.forename: V
authors.initials: V
authors.lastname: Savin
authors.forename: A
authors.initials: A
authors.lastname: Aperia
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D000831:Animals, Newborn / Q000502:physiology; D007668:Kidney / Q000254:growth & development*; D007678:Kidney Glomerulus / Q000254:growth & development* / Q000503:physiopathology; D008297:Male; D009392:Nephrectomy; D051381:Rats; D011919:Rats, Inbred Strains