Sandwich immunoassay for the detection of lipoteichoic acid.
A sandwich immunoassay has been developed for the detection of lipoteichoic acid (LTA), a major cell wall constituent of gram-positive bacteria, from whole blood and ISOLATOR supernate. Monoclonal antibodies were produced to purified LTA from Streptococcus mutans, BHT and were further characterized for crossreactivity with gram-positive and negative bacteria and for reactivity to substituted and unsubstituted LTA. Eight monoclonal antibodies were identified that reacted exclusively with gram-positive bacteria. Those antibodies able to capture 3H-LTA were chosen to develop a sandwich immunoassay. The assay has a sensitivity of 0.2 ng LTA/mL in PBS, 0.5 ng/mL in whole blood and 2.0 ng/mL in whole blood that has been processed through the ISOLATOR. Further development of this assay may lead to the rapid detection of LTA from body fluids.
Tadler
M B
MB
Medical Products Dept., Glasgow Research Lab, E.I. du Pont de Nemours and Co., Wilmington, DE 19898.
Tice
G
G
Wos
S M
SM
D000942:Antigens, Bacterial / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D006090:Gram-Negative Bacteria / Q000032:analysis / Q000276:immunology; D006094:Gram-Positive Bacteria / Q000032:analysis / Q000276:immunology; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008070:Lipopolysaccharides / Q000032:analysis* / Q000097:blood; D018805:Sepsis / Q000097:blood / Q000175:diagnosis; D013682:Teichoic Acids / Q000032:analysis* / Q000097:blood
D000942:Antigens, Bacterial; D008070:Lipopolysaccharides; D013682:Teichoic Acids; C009900:lipoteichoic acid