title: Localization of murine X and autosomal sequences homologous to the human Y located testis-determining region.
abstract: Recently a candidate gene for the primary testis-determining factor (TDF) encoding a zinc finger protein (ZFY) has been cloned from the human Y chromosome. A highly homologous X-linked copy has also been identified. Using this human sequence it is possible to identify two Y loci, an X and an autosomal locus in the mouse (Zfy-1, Zfy-2, Zfx and Zfa, respectively). Suprisingly ZFY is more homologous to the mouse X and autosomal sequences than it is to either of the Y-linked loci. Both Zfy-1 and Zfy-2 are present in the Sxr region of the Y but Zfy-2 is absent in the Sxr deletion variant Sxrb (or Sxr") suggesting it is not necessary for male determination. Extensive backcross analyses map Zfa to mouse chromosome 10 and Zfx to a 5-cM interval between anonymous X probe MDXS120 and the tabby locus (Ta). We also show that the mouse androgen receptor locus (m-AR) believed to underlie the testicular feminization mutation (Tfm) shows complete linkage to Zfx. Comparative mapping indicates that in man these genes lie in separate conserved DNA segments.
authors.affiliation: Unité d'Immunogénétique Humaine, Institut Pasteur, Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Mitchell
authors.forename: D
authors.initials: D
authors.lastname: Simon
authors.forename: N
authors.initials: N
authors.lastname: Affara
authors.forename: M
authors.initials: M
authors.lastname: Ferguson-Smith
authors.forename: P
authors.initials: P
authors.lastname: Avner
authors.forename: C
authors.initials: C
authors.lastname: Bishop
mesh_terms: D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D004268:DNA-Binding Proteins; D005260:Female; D008040:Genetic Linkage; D012739:Gonadal Steroid Hormones / Q000235:genetics*; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011995:Recombination, Genetic; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors; D014960:X Chromosome; D014998:Y Chromosome
chemical_list: D004268:DNA-Binding Proteins; D012739:Gonadal Steroid Hormones; D051741:Kruppel-Like Transcription Factors; D014157:Transcription Factors; C054430:ZFY protein, human; C501342:Zfy1 protein, mouse; C501343:Zfy2 protein, mouse