2'-5' oligo-A-synthetase activity in bovine peripheral blood leukocytes and alveolar macrophages exposed to recombinant interferons and tumor necrosis factor-alpha.
In vitro treatment of bovine peripheral blood mononuclear leukocytes, polymorphonuclear neutrophilic granulocytes and alveolar macrophages with recombinant bovine interferons -alpha 1 1, -beta 2 or -gamma induced an immediate increase in the intracellular level of 2'-5' oligoadenylate synthetase activity. The induction was dose-dependent, with interferon -alpha 1 1 and -beta 2 being more potent than interferon-gamma. Maximal levels were reached within 10-12 h with IFN-alpha 1 1, which corresponded well with findings in vivo. In contrast to what has been found in nonlymphoid bovine cells, tumour necrosis factor-alpha did not potentiate the induction of 2-5A synthetase by interferons, neither did it by itself induce the enzyme.
Ohmann
H B
HB
Veterinary Infectious Disease Organization, Saskatoon, Saskatchewan.
Campos
M
M
Griebel
P J
PJ
Babiuk
L A
LA
D015088:2',5'-Oligoadenylate Synthetase / Q000096:biosynthesis*; D000818:Animals; D002417:Cattle / Q000378:metabolism*; D004790:Enzyme Induction; D007370:Interferon Type I / Q000494:pharmacology*; D007700:Kinetics; D007962:Leukocytes / Q000201:enzymology*; D008264:Macrophages / Q000201:enzymology*; D009504:Neutrophils / Q000201:enzymology; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D007370:Interferon Type I; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D015088:2',5'-Oligoadenylate Synthetase