Microbore liquid chromatography and refractive index gradient detection of low-nanogram and low-ppm quantities of carbohydrates.
A refractive index gradient detector is presented as a universal detector in the microbore high-performance liquid chromatography analysis of carbohydrates. Simultaneously, low-ng and low-ppm injected quantities of carbohydrates were detected at the 3 x root-mean-square baseline noise level. A typical microbore high-performance liquid chromatography chromatogram separating fructose from sucrose followed by refractive index gradient (RIG) detection is reported. Use of a position sensitive detector (PSD) in the RIG detector design is reported and experimental considerations discussed. Optimization of the PSD-based RIG detector is addressed. Potential for the device in industrial and clinical applications is considered.
Hancock
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Department of Chemistry, University of Washington, Seattle 98195.
Synovec
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D002241:Carbohydrates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D012031:Refractometry
D002241:Carbohydrates