title: Fibronectin network recovery in confluent PtK2 cells after acrylamide treatment.
abstract: Confluent PtK2 cells 4 hr treated with 5 mM acrylamide were FN-detected by indirect immunofluorescence. The initial fibrillar-FN network was replaced by an alveolar-type network located at the cell-cell contacts areas in the form of a thick frame with a lace-like appearance. Afterwards, acrylamide removal was obtained by several washes with fresh FCS-free culture medium. Then, PtK2 cells were returned to the incubator for 20 hr. Cell recovery was indicated by reversion of the initial fibrillar-FN network. These data show that FN reversion was possible without any changes in shape and cytoplasmic organization of non-motile growing cells.
authors.affiliation: Centre de Biologie Cellulaire CNRS, Ivry-sur-Seine, France.
authors.forename: J P
authors.initials: JP
authors.lastname: Denèfle
authors.forename: Q L
authors.initials: QL
authors.lastname: Zhu
mesh_terms: D020106:Acrylamide; D000178:Acrylamides / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D005353:Fibronectins / Q000187:drug effects* / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D008856:Microscopy, Fluorescence
chemical_list: D000178:Acrylamides; D005353:Fibronectins; D020106:Acrylamide