Stability of platelet and plasma HLA concentrations in healthy adults or random-donor platelet concentrates.
The quantitative differences between the platelet HLA antigens of different individuals are potentially significant in determining the survival of transfused platelets in allosensitized patients. To learn whether platelet HLA concentration is sufficiently stable to be documented for each donor and used for donor selection, platelet and plasma HLA concentrations in five healthy adults were measured monthly for 5 consecutive months. HLA concentrations were measured by using a competitive enzyme-linked immunoassay. The results showed that both platelet and plasma HLA concentrations varied within +/- 20 percent of the mean values in each individual. Changes in plasma and platelet HLA concentrations were also studied in 5 units of random-donor platelets during in vitro storage. HLA concentrations were determined every other day over a 5-day period. There was no significant quantitative change in the platelet and plasma HLA concentrations during the 5 days of in vitro storage. Thus, platelet HLA concentrations in healthy individuals are stable and may be quantitatively documented for donor selection.
Kao
K J
KJ
Department of Pathology, College of Medicine, University of Florida, Gainesville.
D000328:Adult; D001782:Blood Donors; D001792:Blood Platelets / Q000032:analysis / Q000378:metabolism*; D001793:Blood Preservation; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006680:HLA Antigens / Q000032:analysis*; D006650:Histocompatibility Testing; D006801:Humans; D008297:Male; D017713:Platelet Transfusion; D011897:Random Allocation
D006680:HLA Antigens