title: Human acute lymphoblastic leukemia cell lines with characteristics of intraclonal variation in B-cell differentiation stage.
abstract: The acute lymphoblastic cell lines designated BAL-KHc and BAL-KHs were established from the peripheral blood of a Japanese female patient with a B-cell acute lymphoblastic leukemia. The BAL-KHc and BAL-KHs exhibited B-cell characteristics with positive cell markers for CD19, CD20, CD21 and HLA-DR antigens. Immunoglobulin with gamma and kappa chains was demonstrated on the cultured and fresh leukemia cells respectively. The cells lacked the Epstein-Barr virus genome and expressed abnormal chromosome constitutions including a t(8;14)(q24;q32). These results suggested that the cell lines present B-cell characteristics. The BAL-KHc cells showed different cell growth characteristics and cell surface marker profile compared to those of the BAL-KHs. These variations suggest that the BAL-KHc cells were probably frozen at a different stage of B-cell maturation from those of BAL-KHs, although both cell lines originated from the cells in the same peripheral blood sample of the patient.
authors.affiliation: Department of Immunology, Kurume University School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Hirata
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuo
authors.forename: N
authors.initials: N
authors.lastname: Sadamori
authors.forename: O
authors.initials: O
authors.lastname: Iryo
authors.forename: K
authors.initials: K
authors.lastname: Yoshimoto
authors.forename: M M
authors.initials: MM
authors.lastname: Yokoyama
mesh_terms: D000368:Aged; D000954:Antigens, Surface / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D002051:Burkitt Lymphoma / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D002454:Cell Differentiation; D002460:Cell Line; D002871:Chromosome Banding; D019309:Epstein-Barr Virus Nuclear Antigens; D005260:Female; D006801:Humans; D010641:Phenotype; D012397:Rosette Formation; D014407:Tumor Cells, Cultured / Q000276:immunology / Q000473:pathology* / Q000648:ultrastructure
chemical_list: D000954:Antigens, Surface; D000956:Antigens, Viral; D019309:Epstein-Barr Virus Nuclear Antigens