Lectins for electron microscopic distinction of eosinophils from other blood cells.
Colloidal gold-labeled soybean agglutinin (SBA), Helix pomatia agglutinin (HPA), Dolichos biflorus agglutinin (DBA), and Griffonia simplicifolia lectin (GS-1) were used for electron microscopic observation of blood cells. Colloidal gold-labeled SBA, HPA, and DBA showed marked deposition on eosinophil granules at all stages of maturation. Gold particles were not deposited on basophils, neutrophils, monocytes, lymphocytes, or other blood cells. Only a few colloidal gold-labeled GS-1 were deposited on eosinophil granules. Eosinophil granules are rich in N-acetyl-D-galactosamine compounds, and the colloidal gold-labeled SBA, HPA, and DBA are useful for electron microscopic detection of eosinophil granules.
Eguchi
M
M
Second Department of Pediatrics, Dokkyo University School of Medicine, Tochigi-ken, Japan.
Ozawa
T
T
Suda
J
J
Sugita
K
K
Furukawa
T
T
D000116:Acetylgalactosamine / Q000378:metabolism; D001773:Blood Cells / Q000276:immunology / Q000378:metabolism*; D004804:Eosinophils / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D037102:Lectins / Q000276:immunology / Q000378:metabolism*; D008854:Microscopy, Electron; D037121:Plant Lectins; D030262:Soybean Proteins; D010835:Phytohemagglutinins
D000116:Acetylgalactosamine; D037102:Lectins; D037121:Plant Lectins; D030262:Soybean Proteins; C045475:Griffonia simplicifolia lectins; C038095:Helix lectin; C036554:dolichos biflorus agglutinin; C028824:soybean lectin; D010835:Phytohemagglutinins