Potentiation of fibroblast DNA synthesis by a phenytoin-induced mononuclear cell derived factor in vitro.
Human mononuclear cells purified by Lymphoprep flotation were incubated with phenytoin (PHT) (20 micrograms/ml) or its metabolite p-HPPH (2 micrograms/ml) in the presence of Concanavalin A (10 micrograms/ml) in vitro. The results indicate that phenytoin and its metabolite p-HPPH induce the release of a mononuclear cell factor(s) that activates quiescent human gingival fibroblast to synthesize DNA.
Modéer
T
T
Department of Pedodontics, Odontological Faculty, Karolinska Institute, Huddinge, Sweden.
Dahllöf
G
G
Karsten
J
J
Otteskog
P
P
D002455:Cell Division; D003208:Concanavalin A / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis*; D005347:Fibroblasts / Q000378:metabolism*; D005881:Gingiva / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D010672:Phenytoin / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology
D010835:Phytohemagglutinins; D003208:Concanavalin A; D010672:Phenytoin; D004247:DNA