title: Activation and peroxisomal beta-oxidation of fatty acids and bile acid intermediates in liver from Bombina orientalis and from the rat.
abstract: 1. Bombina orientalis excretes mainly C27 bile acids: trihydroxycoprostanic and varanic acids. More than 90% of the trihydroxycoprostanic acid (THCA) present in the bile, was conjugated with taurine; varanic acid was present in the unconjugated form. 2. Trihydroxycoprostanoyl-CoA (THC-CoA) synthetase activity, required for the formation of the taurine conjugate, was present in the liver of Bombina orientalis. 3. Peroxisomal beta-oxidation, which catalyzes the oxidation of fatty acids as well as the conversion of C27 bile acids into C24 bile acids in rat and human liver, could be detected in liver of Bombina orientalis when palmitoyl-CoA was used as substrate, but not when trihydroxycoprostanoyl-CoA (THC-CoA) was used.
authors.affiliation: Afdeling Farmakologie, Katholieke Universiteit Leuven, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Casteels
authors.forename: L
authors.initials: L
authors.lastname: Schepers
authors.forename: G
authors.initials: G
authors.lastname: Parmentier
authors.forename: H J
authors.initials: HJ
authors.lastname: Eyssen
authors.forename: G P
authors.initials: GP
authors.lastname: Mannaerts
mesh_terms: D000818:Animals; D001001:Anura / Q000378:metabolism*; D001647:Bile Acids and Salts / Q000378:metabolism*; D002793:Cholic Acids / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D008297:Male; D008830:Microbodies / Q000378:metabolism; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity
chemical_list: D001647:Bile Acids and Salts; D002793:Cholic Acids; D005227:Fatty Acids; C003681:3,7,12-trihydroxycoprostanic acid