The oncogenicity of avian adenoviruses. IV. Confirmatory evidence for recombination between viral and cellular DNA sequences and repetition of the recombinant in cells of a tumor line.
On the basis of hybridization analyses with viral DNA fragments as probes, we previously concluded that, in DNAs of many fowl adenovirus type 1-induced rodent tumors, units consisting of viral DNA segments flanked on both sides by cellular DNA segments are repeated more than 100 times per diploid genome of cell. Molecular cloning of virus-cellular junction fragments and analyses of their sequence arrangement in the present study confirmed that the previous conclusion was basically correct.
Yasue
H
H
Laboratory of Viral Oncology, Aichi Cancer Center, Nagoya, Japan.
Iwami
M
M
Koide
Y
Y
Ohtsubo
E
E
Ishibashi
M
M
D000256:Adenoviridae / Q000235:genetics / Q000472:pathogenicity*; D015139:Blotting, Southern; D002472:Cell Transformation, Viral; D003001:Cloning, Molecular; D011995:Recombination, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping