[Use of a gelatin substrate for culturing diploid human skin fibroblasts].
Gelatin coating of the growth surface of commercially available polystyrene Petri dishes is proposed for successful cultivation of human skin fibroblasts. A comparison of culture growth dynamics on gelatin, glass and polystyrene allowed to recommend gelatin as the substrate in order to receive an abundant similar material with a low population density viability. The absence of changes in carbohydrate metabolism changes during cell culturing on gelatin provides an opportunity to use these cells in studying regulation of carbohydrate metabolism.
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Evdokimova
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D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media; D004171:Diploidy; D005347:Fibroblasts / Q000166:cytology; D005780:Gelatin; D005898:Glass; D006801:Humans; D011137:Polystyrenes; D012867:Skin / Q000166:cytology*; D013997:Time Factors
D003470:Culture Media; D011137:Polystyrenes; D005780:Gelatin
Primenenie zhelatinovogo substrata dlia kul'tivirovaniia diploidnykh fibroblastov kozhi cheloveka.