Factors influencing the differentiation of embryonal carcinoma and embryo-derived stem cells.
The effects of aggregation, retinoic acid, and medium conditioned by Buffalo rat liver (BRL) cells, alone and in combination, on the differentiation of PSA4TG12 embryonal carcinoma and E14 embryonal stem cells are reported. The observations indicate that BRL-conditioned medium has more than one effect on the differentiation process, that retinoic acid has at least two effects which operate in different concentration ranges, and that both agents influence the choice of differentiation pathway as well as the extent of differentiation.
Fisher
J P
JP
Department of Pathology, University of Edinburgh Medical School, United Kingdom.
Hope
S A
SA
Hooper
M L
ML
D000818:Animals; D002449:Cell Aggregation; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002999:Clone Cells; D003470:Culture Media; D004622:Embryo, Mammalian / Q000166:cytology*; D008099:Liver / Q000502:physiology; D051379:Mice; D008854:Microscopy, Electron; D051381:Rats; D011915:Rats, Inbred BUF; D013724:Teratoma / Q000473:pathology* / Q000648:ultrastructure; D014212:Tretinoin / Q000494:pharmacology
D003470:Culture Media; D014212:Tretinoin