Covalent coupling of sugars to liposomes.
A procedure is described for the covalent coupling of p-aminophenyl-alpha-D-mannopyranoside, in the presence of carbodiimide, to a derivative of phosphatidylethanolamine (N-glutarylphosphatidylethanolamine) incorporated into the bilayers of multilamellar liposomes prepared by the dehydration-rehydration method. It appears that much of the phospholipid derivative exposed on the surface of the outer liposomal bilayer interacts with the aminosugar. The procedure is simple, does not destabilize liposomes and could be applied to other receptor-specific aminosugars.
Weissig
V
V
Medical Research Council Group, Royal Free Hospital School of Medicine, London, U.K.
Lasch
J
J
Gregoriadis
G
G
D001665:Binding Sites; D055598:Chemical Phenomena; D002621:Chemistry; D008081:Liposomes; D008358:Mannose; D010539:Permeability
D008081:Liposomes; D008358:Mannose