title: Determination of xylometazoline in plasma and urine by gas chromatography using a fused-silica capillary column and an electron-capture detector.
abstract: A sensitive method is described for the determination of unchanged xylometazoline in plasma and urine at concentrations down to 35 nmol/l. After addition of naphazoline as an internal standard, both compounds are extracted with dichloromethane-diethyl ether (20:80) at pH 10, back-extracted with an acidic solution and re-extracted from a sodium hydroxide solution with dichloromethane-diethyl ether (20:80). The compounds are then derivatized with heptafluorobutyric anhydride in the presence of pyridine. The derivatives are determined by capillary gas chromatography using electron-capture detection.
authors.affiliation: Laboratoires Ciba-Geigy, Biopharmaceutical Research Centre, Rueil-Malmaison, France.
authors.forename: A
authors.initials: A
authors.lastname: Sioufi
authors.forename: F
authors.initials: F
authors.lastname: Leroux
authors.forename: J P
authors.initials: JP
authors.lastname: Dubois
mesh_terms: D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D006801:Humans; D007093:Imidazoles / Q000097:blood / Q000378:metabolism* / Q000652:urine; D012015:Reference Standards
chemical_list: D007093:Imidazoles; C009695:xylometazoline