title: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis without a stacking gel: use for high sample throughput.
abstract: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis without a stacking gel minimizes lateral spreading of protein when samples are applied in agarose wells and allows high sample throughput (6 samples/cm gel width). The method is simple and convenient to use and gives comparable resolution to the standard method with 4-20% or 6-30% polyacrylamide gradient gels. Best results are obtained when the upper zone of the separating gel is of low polyacrylamide concentration. This indicates a need for the molten agarose to penetrate and anneal with the separating gel.
authors.affiliation: Biology Department, The University of Ulster, Coleraine, Northern Ireland.
authors.forename: T
authors.initials: T
authors.lastname: Marshall
authors.forename: S J
authors.initials: SJ
authors.lastname: Gaston
authors.forename: K M
authors.initials: KM
authors.lastname: Williams
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D011506:Proteins / Q000032:analysis*; D012685:Sepharose; D012967:Sodium Dodecyl Sulfate
chemical_list: D011506:Proteins; D012967:Sodium Dodecyl Sulfate; D012685:Sepharose