Isolation of a Legionella pneumophila restriction mutant with increased ability to act as a recipient in heterospecific matings.
The ability of Legionella pneumophila to act as a recipient of IncP and IncQ plasmids in matings with Escherichia coli varies widely from strain to strain. We found that the low efficiency of mating of the Philadelphia-1 strain is due to a type II restriction-modification system, and we isolated and characterized a Philadelphia-1 mutant that lacks the restriction enzyme activity.
Marra
A
A
Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, New York 10032.
Shuman
H A
HA
D003227:Conjugation, Genetic; D015280:DNA Restriction-Modification Enzymes / Q000235:genetics*; D007875:Legionella / Q000235:genetics*; D009154:Mutation; D010957:Plasmids
D015280:DNA Restriction-Modification Enzymes