Reconstruction from stereo and multiple tilt electron microscope images of thick sections of embedded biological specimens using computer graphic methods.
The extraction and display of 3-D information using modern computer graphic equipment in the electron microscope is presented. Thick specimens were imaged at 400 kV. Modelling of vectors and the tilting of surfaces is discussed as is the accuracy of reconstructions.
Peachey
L D
LD
Department of Biology, University of Pennsylvania, Philadelphia 19104-6019.
Heath
J P
JP
D000818:Animals; D003196:Computer Graphics; D003198:Computer Simulation; D005347:Fibroblasts / Q000648:ultrastructure*; D007150:Immunohistochemistry; D008854:Microscopy, Electron / Q000379:methods*; D008954:Models, Biological; D009132:Muscles / Q000648:ultrastructure*