Dimeric dihydrodiol dehydrogenase in monkey kidney. Substrate specificity, stereospecificity of hydrogen transfer, and distribution.
Chemical cross-linking and NADPH binding studies suggested that the native dihydrodiol dehydrogenase from monkey kidney is a basic dimer having a molecular weight of 78,000 and one active site per the subunit. The enzyme oxidized specifically trans-dihydrodiols of benzene and naphthalene, whereas it catalyzed the reduction of dihydroxyacetone and dihydroxyacetone phosphate at a physiological pH, 7.4. The Km and kcat values for dihydroxyacetone phosphate were 5.0 mM and 4.3 s-1, respectively. The enzyme transferred the 4-pro-R hydrogen atom of NADPH to the carbonyl substrate. Immunochemical experiments using an antibody against the dimeric enzyme revealed the specific distribution of the enzyme in the kidney of this animal. By immunohistochemical staining with the specific antibody, the immunoreactivity was found in proximal and distal tubules of the cortex, and in the loop of Henle of the medulla.
Nakagawa
M
M
Department of Biochemistry, Gifu Pharmaceutical University.
Matsuura
K
K
Hara
A
A
Sawada
H
H
Bunai
Y
Y
Ohya
I
I
D000429:Alcohol Oxidoreductases / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D000906:Antibodies / Q000276:immunology; D003432:Cross-Linking Reagents / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism; D007668:Kidney / Q000201:enzymology*; D008251:Macaca; D008252:Macaca fascicularis; D008253:Macaca mulatta; D008297:Male; D015394:Molecular Structure; D008970:Molecular Weight; D009249:NADP / Q000378:metabolism; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; D013237:Stereoisomerism; D013379:Substrate Specificity; D014018:Tissue Distribution
D000906:Antibodies; D003432:Cross-Linking Reagents; D009249:NADP; D006859:Hydrogen; D010088:Oxidoreductases; D000429:Alcohol Oxidoreductases; C023125:dihydrodiol dehydrogenases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C116788:cis-1,2-dihydro-1,2-dihydroxynaphthalene dehydrogenase