title: Phosphorylation of fructose bisphosphate aldolase in Trypanosoma brucei.
abstract: Methods were developed for in vivo labelling of trypanosome proteins with inorganic phosphate and the labelling of fructose bisphosphate aldolase and variant surface glycoprotein was investigated. It was found that the large pool size of phosphate in trypanosomes precludes detailed kinetic analyses. Aldolase contains low levels of phosphoserine but the function of this phosphorylation has yet to be determined.
authors.affiliation: Rockefeller University, New York, NY 10021.
authors.forename: C E
authors.initials: CE
authors.lastname: Clayton
authors.forename: J A
authors.initials: JA
authors.lastname: Fox
mesh_terms: D000818:Animals; D001345:Autoradiography; D002458:Cell Fractionation; D004591:Electrophoresis, Polyacrylamide Gel; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism*; D007553:Isotope Labeling; D007700:Kinetics; D010766:Phosphorylation; D011233:Precipitin Tests; D051381:Rats; D014346:Trypanosoma brucei brucei / Q000201:enzymology / Q000378:metabolism*; D014643:Variant Surface Glycoproteins, Trypanosoma / Q000378:metabolism
chemical_list: D014643:Variant Surface Glycoproteins, Trypanosoma; D005634:Fructose-Bisphosphate Aldolase