[Catalytic activity of conjugates of peroxidase with progesterone in aqueous and micellar media in the presence of antibodies against progesterone].
At 22 and 41 degrees C the horseradish peroxidase (HRP) conjugates with progesterone, HRP-PROG-1 and HRP-PROG-2, were obtained by HRP reaction with N-hydroxysuccinimide ester of 3-O-carboxymethyloxime of progesterone. The interaction of these conjugates and unmodified enzyme with antibodies against progesterone was studied in phosphate-citrate buffer pH 3.6 and in the reversed micelles of Aerosol OT and Triton X-45 in heptane. Catalytic function of conjugates in buffered solutions and in reversed micelles increases as result of the conjugates' interaction with antibodies. The free progesterone in reversed micelles competes with HRP-PROG-1 for antibody binding, which may be the basis for homogeneous enzyme-immunoassay of progesterone, depending on the micelle composition, the antibody concentration and temperature.
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D000906:Antibodies / Q000276:immunology*; D001667:Binding, Competitive; D002384:Catalysis; D006735:Horseradish Peroxidase / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D008823:Micelles; D010544:Peroxidases / Q000378:metabolism*; D011374:Progesterone / Q000276:immunology / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013501:Surface-Active Agents; D013696:Temperature; D014867:Water
D000906:Antibodies; D008823:Micelles; D013501:Surface-Active Agents; D014867:Water; D012710:Serum Albumin, Bovine; D011374:Progesterone; D006735:Horseradish Peroxidase; D010544:Peroxidases
Kataliticheskaia aktivnost' kon''iugatov peroksidazy s progesteronom v vodnykh i mitselliarnykh sredakh v prisutstvii antitel protiv progesterona.