title: Effects of dexamethasone on expression and maintenance of cartilage in serum-containing cultures of calvaria cells.
abstract: The effects of dexamethasone on the ability of cells enzymatically isolated from 21-day fetal rat calvaria to produce cartilage in vitro has been investigated. Primary cultures of single-cell suspensions of rat calvaria were grown for up to 28 days in vitro in alpha-minimal essential medium containing 15% fetal bovine serum, 50 micrograms/ml ascorbic acid, 10 mM Na beta-glycerophosphate and dexamethasone at concentrations of 1 microM to 1 nM. Two types of nodules were present in dexamethasone-containing cultures. One has been characterized previously as bone (Bellows et al. 1986). The second morphologically resembled hyaline cartilage, possessed a strong Alcian blue-positive matrix and contained type-II, but not type-I, collagen. Both bone and cartilaginous nodules were spatially distinct and developed in isolation from each other. Cartilaginous nodules were found in the highest number at a dexamethasone concentration of 100 nM. Time-course experiments revealed that while the number of bone nodules increased continuously at least to day 28, the number of cartilaginous nodules remained constant after cultures had reached confluency. When cells were isolated separately from frontal and parietal bones and sutural regions, the greatest number of cartilaginous nodules developed from parietal bones. Since 21-day fetal rat calvaria contains 2 distinct patches of cartilage at the periphery of the parietal bones, it seems likely that this cartilaginous tissue is the origin of the cartilage cells. The results demonstrate that cultures of rat calvaria cells contain chondrocytes and possibly chondroprogenitor cells that are distinct from osteoprogenitors.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Medical Research Council Group in Periodontal Physiology, Faculty of Dentistry, University of Toronto, Ontario, Canada.
authors.forename: C G
authors.initials: CG
authors.lastname: Bellows
authors.forename: J N
authors.initials: JN
authors.lastname: Heersche
authors.forename: J E
authors.initials: JE
authors.lastname: Aubin
mesh_terms: D000818:Animals; D002356:Cartilage / Q000166:cytology* / Q000187:drug effects; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012886:Skull / Q000166:cytology*; D013997:Time Factors
chemical_list: D003907:Dexamethasone