Biosynthesis of adrenal corticosteroids by the sesterterpene pathway via 23,24-dinor-4-cholen-3-one.
Rat adrenal gland preparations were incubated with 23,24-dinor-5-cholen-3 beta-ol (Guneribol), a proposed intermediate in the sesterterpene pathway for steroid biosynthesis. Steroids were isolated, purified by thin layer and high-performance liquid chromatographies and crystallized to constant specific activity. These preparations converted the substrate to 23,24-dinor-4-cholen-3-one. Radioactive 23,24-dinor-4-cholen-3-one was synthesised and incubated with further tissue preparations and shown to be converted to corticosteroids. These findings suggest that 23,24-dinor-4-cholen-3-one is an intermediate on the sesterterpene pathway for steroidogenesis.
Tait
A D
AD
Department of Obstetrics & Gynaecology, University of Cambridge, Addenbrooke's Hospital, England.
Hodge
L C
LC
Abbs
D
D
D000305:Adrenal Cortex Hormones / Q000096:biosynthesis* / Q000302:isolation & purification; D000311:Adrenal Glands / Q000378:metabolism*; D000818:Animals; D002250:Carbon Radioisotopes; D002784:Cholesterol / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism; D011283:Pregnenes / Q000378:metabolism*; D011865:Radioisotope Dilution Technique; D051381:Rats; D054830:Sesterterpenes; D013729:Terpenes / Q000378:metabolism*; D014316:Tritium
D000305:Adrenal Cortex Hormones; D002250:Carbon Radioisotopes; D011283:Pregnenes; D054830:Sesterterpenes; D013729:Terpenes; D014316:Tritium; C061511:guneribone; D002784:Cholesterol