Toward a steady-state pore limit electrophoresis dimension for native proteins in two-dimensional polyacrylamide gel electrophoresis.
Polyacrylamide gel electrophoresis in linear pore gradients (4.8 to 48% T, 5% CBis) provides for migration arrest, in a practical sense, after about 5000 Vh for proteins of 290 and 450 kDa, but not for smaller proteins over 20,000 Vh. The arrest is not due to inadequate field strength nor is it caused by water redistribution within pore gradient gels. The possibility is being discussed that exponential pore gradients, and a higher or a lower degree of crosslinking suggested by the literature may be remedies for the present failure to arrest the migration of smaller proteins.
Fawcett
J S
JS
Section on Macromolecular Analysis, National Institute of Child Health and Human Development, Bethesda, MD 20892.
Sullivan
J V
JV
Chrambach
A
A
D000180:Acrylic Resins; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004574:Electromagnetic Fields; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D008970:Molecular Weight; D010316:Particle Size; D011506:Proteins / Q000302:isolation & purification; D014867:Water
D000180:Acrylic Resins; D011506:Proteins; C016680:polyacrylamide gels; D014867:Water