The involvement of dimethyl sulfoxide in a bacteriotoxic response of the Ames assay tester strains TA98 and TA100.
Dimethyl sulfoxide is a widely accepted and recommended solvent in which to dissolve compounds to be tested for mutagenicity via the Ames Salmonella/mammalian microsome assay. Using tester strains TA98 and TA100, we observed a bacteriotoxic response with various fractions isolated from beer when dissolved in DMSO but not when dissolved in water. Further characterization of the role of solvent in simple model systems consisting of butanol, DMSO and bacteria strongly suggests a chemical reaction occurs between dimethyl sulfoxide and specific chemical constituents of the test substance, nutrient broth, or the Ames bacterial strains. The result of such an interaction could be misinterpreted as a toxic response to the test substance when, in fact, the bacteriotoxicity could be due to another compound, chemically distinct from the test substance.
Vaughan
D J
DJ
Ludwig Institute for Cancer Research, Toronto, Ont., Canada.
Baptista
J A
JA
Perdomo
G R
GR
Krepinsky
J J
JJ
D020001:1-Butanol; D000440:Butanols / Q000494:pharmacology; D002384:Catalysis; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000494:pharmacology*; D010084:Oxidation-Reduction; D012475:Salmonella / Q000187:drug effects*; D012997:Solvents / Q000494:pharmacology*
D000440:Butanols; D009153:Mutagens; D012997:Solvents; C043958:2-butanol; D020001:1-Butanol; D004121:Dimethyl Sulfoxide