A novel type of human alpha-amylase produced in lung carcinoid tumor.
A novel type of alpha-amylase was detected in a lung carcinoid tissue after surveying the cDNA library constructed from this tumor mRNA. Nucleotide sequence analysis showed that the amylase expressed in this carcinoid tumor has 13 and 6 amino acid substitutions when compared with salivary amylase (Amy1) and pancreatic amylase (Amy2), respectively. The nucleotide sequence homologies of cDNAs between this carcinoid amylase and amy1, amy2 are 97.5% and 98.2%, respectively. The nucleotide sequence comparison strongly suggests that this new amylase is the product of the amy3 gene that has been detected in human genome [Emi et al., Gene 62 (1988) 229-235]
Tomita
N
N
Institute for Molecular and Cellular Biology, Osaka University, Japan.
Horii
A
A
Doi
S
S
Yokouchi
H
H
Shiosaki
K
K
Higashiyama
M
M
Matsuura
N
N
Ogawa
M
M
Mori
T
T
Matsubara
K
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D002276:Carcinoid Tumor / Q000201:enzymology*; D003001:Cloning, Molecular; D003062:Codon; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D004273:DNA, Neoplasm; D005260:Female; D005796:Genes; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000235:genetics / Q000302:isolation & purification; D008175:Lung Neoplasms / Q000201:enzymology*; D008875:Middle Aged; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D000516:alpha-Amylases / Q000235:genetics* / Q000302:isolation & purification
D003062:Codon; D015342:DNA Probes; D004273:DNA, Neoplasm; D007527:Isoenzymes; D012333:RNA, Messenger; D004247:DNA; D000516:alpha-Amylases