Primary structure and transcriptional regulation of rat pepsinogen C gene.
The entire rat pepsinogen C gene has been isolated from a rat genomic library, using the rat pepsinogen C cDNA as a probe. Southern blot analysis showed that there exists at least two rat pepsinogen C genes. The nucleotide sequences of the coding regions and the 5'- and 3'-flanking regions of one of the rat pepsinogen C genes have been determined. This gene is split into 9 exons interrupted by eight intervening sequences. The 5'-flanking region is similar to that of the human pepsinogen C gene, but only the former has the core sequence of the Sp1 binding site. The amount of transcripts of the rat pepsinogen C genes was found to increase during development, and a similar increase was shown to be induced by injection of hydrocortisone. As a candidate of a factor which regulates the transcription, we found a 25-kDa protein by Southwestern blotting. It binds to a specific site in the 5'-flanking region of the gene only in the presence of Mg2+ ion, and it is present in the nuclear fraction of the gastric mucosa but not of the liver.
Ishihara
T
T
Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan.
Ichihara
Y
Y
Hayano
T
T
Katsura
I
I
Sogawa
K
K
Fujii-Kuriyama
Y
Y
Takahashi
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005796:Genes; D005809:Genes, Regulator; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010435:Pepsinogens / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D015183:Restriction Mapping; D014158:Transcription, Genetic
D010435:Pepsinogens; D004247:DNA