Expression of hepatitis B virus large envelope protein in Escherichia coli and Saccharomyces cerevisiae.
The gene coding for hepatitis B large envelope protein was cloned under the lac promoter in bacterial vector pUC-8 and under the ADH1 promoter in yeast expression shuttle vector pVT103-U, and expression of HBsAg in bacteria and yeast was determined. The strongest expression of large envelope protein was obtained after transformation of the protease-deficient yeast strain BJ1991. The recombinant large envelope protein did not form complex 22-nm particles and was not secreted into medium.
Korec
E
E
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Praha.
Korcová
J
J
Palková
Z
Z
Vondrejs
V
V
Korínek
V
V
Reinis
M
M
Bichko
V V
VV
Hlozánek
I
I
D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D005814:Genes, Viral; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D006515:Hepatitis B virus / Q000235:genetics*; D007124:Immunoenzyme Techniques; D010957:Plasmids / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014169:Transformation, Bacterial; D014461:Ultracentrifugation; D014759:Viral Envelope Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification
D006514:Hepatitis B Surface Antigens; D014759:Viral Envelope Proteins