[Complete inhibition of cyclic transmission by immunization against Plasmodium berghei Anka ookinetes].
An antiserum raised against P. berghei Anka ookinetes binds specifically to ookinetes and zygotes surface as assessed by indirect immunofluorescence. The transmission blocking activity of this serum was assessed by feeding mosquitoes on immunized mice. A serum showing an antibody titer of 1/320 reached after 6 immunizations completely blocks ookinetes development. This immune serum might be used to screen P. berghei genomic or cDNA library in order to identify or clone sequences involved in the process of gametocyte infectivity.
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Laboratoire de Protozoologie, Institut de Médecine Tropicale, Antwerpen, Belgique.
Wery
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D000818:Animals; D000852:Anopheles / Q000469:parasitology; D000913:Antibodies, Protozoan / Q000032:analysis; D005455:Fluorescent Antibody Technique; D007106:Immune Sera / Q000276:immunology; D007114:Immunization; D008288:Malaria / Q000276:immunology / Q000517:prevention & control / Q000635:transmission; D051379:Mice; D010962:Plasmodium berghei / Q000276:immunology* / Q000502:physiology; D013997:Time Factors
D000913:Antibodies, Protozoan; D007106:Immune Sera
Inhibition complète de la transmission cyclique par immunisation contre les ookinètes de Plasmodium berghei Anka.