title: Elimination of hydroxypropyl methylcellulose from the anterior chamber of the rabbit.
abstract: The elimination of hydroxypropyl methylcellulose (HPMC) of molecular weight 86,000 daltons (2%, viscosity 4,000 centipoises; 2.5%, 15,000 centipoises) and 120,000 daltons (2%, 15,000 centipoises) from the anterior chamber of rabbit eyes was determined colorimetrically and was nearly complete by 24 hours. The elimination rate was dependent on the viscosity of the HPMC injected into the eye. Elevation of the intraocular pressure by HPMC was detected three hours after injection, but the pressure was almost normal at six hours and was normal thereafter. This effect is independent of the molecular weight, the concentration, and the viscosity of the solutions used. Using an in vitro assay method, HPMC (86,000 daltons) at 2.5% concentration in tissue culture medium was innocuous to rabbit endothelial cells.
authors.affiliation: Eye Research Institute, Harvard Medical School, Boston, Massachusetts 02114.
authors.forename: J
authors.initials: J
authors.lastname: Fernandez-Vigo
authors.forename: M F
authors.initials: MF
authors.lastname: Refojo
authors.forename: M
authors.initials: M
authors.lastname: Jumblatt
mesh_terms: D000818:Animals; D000869:Anterior Eye Segment / Q000187:drug effects / Q000378:metabolism* / Q000601:surgery; D001082:Aqueous Humor / Q000032:analysis; D004728:Endothelium, Corneal / Q000187:drug effects; D005260:Female; D065347:Hypromellose Derivatives; D007429:Intraocular Pressure / Q000187:drug effects*; D008297:Male; D008747:Methylcellulose / Q000031:analogs & derivatives* / Q000032:analysis / Q000493:pharmacokinetics / Q000494:pharmacology; D011817:Rabbits
chemical_list: D065347:Hypromellose Derivatives; D008747:Methylcellulose