Proton nuclear magnetic resonance studies on the variant-3 neurotoxin from Centruroides sculpturatus Ewing: sequential assignment of resonances.
We report the sequential assignment of resonances to specific residues in the proton nuclear magnetic resonance spectrum of the variant-3 neurotoxin from the scorpion Centruroides sculpturatus Ewing (range southwestern U.S.A.). A combination of two-dimensional NMR experiments such as 2D-COSY, 2D-NOESY, and single- and double-RELAY coherence transfer spectroscopy has been employed on samples of the protein dissolved in D2O and in H2O for assignment purposes. These studies provide a basis for the determination of the solution-phase conformation of this protein and for undertaking detailed structure-function studies of these neurotoxins that modulate the flow of sodium current by binding to the sodium channels of excitable membranes.
Nettesheim
D G
DG
Comprehensive Cancer Center, University of Alabama, Birmingham 35294.
Klevit
R E
RE
Drobny
G
G
Watt
D D
DD
Krishna
N R
NR
D000595:Amino Acid Sequence; D000818:Animals; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D009498:Neurotoxins; D011487:Protein Conformation; D012604:Scorpion Venoms / Q000302:isolation & purification
C035413:Centruroides toxin; D009498:Neurotoxins; D012604:Scorpion Venoms