title: High degree of homology between primary structure of human lysosomal acid phosphatase and human prostatic acid phosphatase.
abstract: Alignment of the amino-acid sequences of the human lysosomal acid phosphatase (LAP) and human prostatic acid phosphatase (PAP) yielded an extensive homology between the two mature polypeptide chains. In the overlapping part, which extends over the entire PAP sequence and the N-terminal 90% of the LAP sequence, the identity is 49.1%. The LAP has an additional C-terminal sequence, which is encoded by the last exon of the LAP gene. This sequence contains the transmembrane domain of LAP, which is lacking in the secretory PAP. All six cysteine residues as well as 20 out of 27 (LAP) and 26 (PAP) proline residues present in the overlapping part of the proteins are conserved, suggesting that they are involved in stabilization of the tertiary structure of both proteins. Only two out of 8 N-glycosylation sites in LAP and 3 in PAP are conserved, suggesting that the dense N-glycosylation of LAP is related to its function in lysosomes.
authors.affiliation: Zentrum Biochemie der Universität Göttingen, Biochemie II, Finland.
authors.forename: C
authors.initials: C
authors.lastname: Peters
authors.forename: C
authors.initials: C
authors.lastname: Geier
authors.forename: R
authors.initials: R
authors.lastname: Pohlmann
authors.forename: A
authors.initials: A
authors.lastname: Waheed
authors.forename: K
authors.initials: K
authors.lastname: von Figura
authors.forename: K
authors.initials: K
authors.lastname: Roiko
authors.forename: P
authors.initials: P
authors.lastname: Virkkunen
authors.forename: P
authors.initials: P
authors.lastname: Henttu
authors.forename: P
authors.initials: P
authors.lastname: Vihko
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism*; D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA / Q000032:analysis; D006801:Humans; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011467:Prostate / Q000201:enzymology*
chemical_list: D004247:DNA; D000135:Acid Phosphatase