title: Cytogenetic studies of Hodgkin's disease. Analysis of involved lymph nodes from 12 patients.
abstract: Cytogenetic studies were performed on 12 involved lymph nodes from Hodgkin's disease patients utilizing conditioned medium from 12-O-tetradecanoylphorbol-13-acetate-staphylococcus enterotoxin A induced mononuclear cells. The majority of cells analyzed had a normal karyotype. An unusually high rate of nonclonal karyotypic abnormalities was noted in most cultures. Clonal abnormalities involving chromosomes 3 and 21 were noted in two patients. Cytogenetic analysis of cultures stimulated with conditioned medium or specific growth factors may lead to a better understanding of the genetic mechanisms involved in Hodgkin's disease.
authors.affiliation: Department of Pathology, University of Nevada School of Medicine Genetics, Network, Reno 89502.
authors.forename: T R
authors.initials: TR
authors.lastname: Dennis
authors.forename: A D
authors.initials: AD
authors.lastname: Stock
authors.forename: C D
authors.initials: CD
authors.lastname: Winberg
authors.forename: K
authors.initials: K
authors.lastname: Sheibani
authors.forename: H
authors.initials: H
authors.lastname: Rappaport
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D002648:Child; D002869:Chromosome Aberrations; D002891:Chromosomes, Human, Pair 21; D002893:Chromosomes, Human, Pair 3; D005260:Female; D006689:Hodgkin Disease / Q000235:genetics*; D006801:Humans; D008198:Lymph Nodes / Q000648:ultrastructure*; D008297:Male; D008875:Middle Aged; D012730:Sex Chromosomes