Immunity in mice vaccinated with a molecular weight 60,000 glycoprotein secreted by adult Nematospiroides dubius.
A mol. wt 60,000 glycoprotein was purified from adult Nematospiroides dubius excretory-secretory products (ES) by polyacrylamide gel electrophoresis and electroelution and used to vaccinate BCF1 mice. This molecule is also released from the surface of the parasite and can be cleaved by pepsin. Mice immunized twice with ES 60,000 in Alum adjuvant harboured similar numbers of N. dubius as did control mice but voided 40% fewer parasite eggs in their faeces. The mol. wt 60,000 component in ES and from the surface of the parasite appears to influence how the parasite reproduces and may be important to its survival.
Monroy
F G
FG
East
I J
IJ
Dobson
C
C
Adams
J H
JH
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D005260:Female; D006023:Glycoproteins / Q000276:immunology*; D006369:Heligmosomatoidea / Q000276:immunology*; D051379:Mice; D009349:Nematode Infections / Q000517:prevention & control*; D009351:Nematospiroides dubius / Q000276:immunology*; D014611:Vaccination
D000947:Antigens, Helminth; D000954:Antigens, Surface; D006023:Glycoproteins