title: An enzymic assay for uric acid in serum and urine compared with HPLC.
abstract: We evaluated a colorimetric method for the assay of uric acid in serum or urine, which utilises a Trinder chromogenic system modified by the inclusion of 2,4,6-tribromo-3-hydroxybenzoic acid for oxidative coupling to p-aminophenazone. Colour development (Amax: 512 nm) is complete within five minutes. Measurement of a sample blank is not needed. The procedure involves pre-incubation with ascorbic acid oxidase and detergent to eliminate interference by ascorbic acid and to abolish turbidity due to lipaemia; this pretreatment was effective up to 1.14 mmol/l ascorbate and up to at least 25 mmol/l triacylglycerol. Interference by icteric sera was insignificant up to about 170 mumol/l bilirubin. The method is linear up to at least 1428 mumol/l. In human serum and urine the procedure correlates well with HPLC and the uricase p-aminophenazone method on the SMAC analyser. Within-run and between-run imprecisions of the enzymic test were higher than for HPLC, but did not exceed 1.2% (CV) and 2.5% (CV), respectively.
authors.affiliation: Boehringer Mannheim GmbH, FRG.
authors.forename: H
authors.initials: H
authors.lastname: Dubois
authors.forename: B
authors.initials: B
authors.lastname: Delvoux
authors.forename: V
authors.initials: V
authors.lastname: Ehrhardt
authors.forename: H
authors.initials: H
authors.lastname: Greiling
mesh_terms: D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D014527:Uric Acid / Q000097:blood / Q000378:metabolism* / Q000652:urine
chemical_list: D014527:Uric Acid