Sensitive ferrocene reagents for derivatization of amines for high-performance liquid chromatography with electrochemical detection.
Six reagents possessing ferrocene as an electrophore were prepared and evaluated for pre-column derivatization of amino compounds for their determination by high-performance liquid chromatography with electrochemical detection. The utility of these reagents was investigated employing phenethylamine as a model compound. Among these six, N-succinimidyl 3-ferrocenylpropionate was the best with respect to reactivity, stability and electrochemical properties. The developed method was applied to the determination of putrescine formed from ornithine by ornithine decarboxylase.
Shimada
K
K
Pharmaceutical Institute, Tohoku University, Sendai, Japan.
Oe
T
T
Tanaka
M
M
Nambara
T
T
D000588:Amines / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D005296:Ferrous Compounds; D007202:Indicators and Reagents; D000075163:Metallocenes; D009942:Organometallic Compounds; D009952:Ornithine / Q000032:analysis; D009955:Ornithine Decarboxylase / Q000378:metabolism; D011700:Putrescine / Q000032:analysis
D000588:Amines; D005296:Ferrous Compounds; D007202:Indicators and Reagents; D000075163:Metallocenes; D009942:Organometallic Compounds; D009952:Ornithine; D009955:Ornithine Decarboxylase; C004998:ferrocene; D011700:Putrescine