title: Suppressing effect of cysteamine on the TSH-stimulated mitotic activity of the rat thyroid follicular cells in vivo.
abstract: We have examined the effect of the thiol agent-cysteamine, known to deplete the hypothalamus, the pancreas, the gut of somatostatin--on the basal and TSH-stimulated mitotic activity of the thyroid follicular cells (TFC) in adult male rats in vivo. The animals of different groups were administered cysteamine alone (300 mg/kg BW, s.c.), TSH alone (2IU, i.p.), or cysteamine with TSH jointly, the former agent being injected 2 hrs prior to the latter. In order to evaluate the thyroid mitotic activity, the metaphase-arrest technique was used in the study. It was shown that: 1. TSH significantly increased the mitotic activity of TFC, as compared to controls, 2. cysteamine alone had no effect on the TFC proliferation, 3. unexpectedly, cysteamine, when administered 2 hrs prior to TSH, suppressed the stimulatory effect of TSH on the mitotic activity of TFC.
authors.affiliation: Department of Experimental Endocrinology and Hormone Diagnostics, Medical Academy of Lodz, Poland.
authors.forename: G
authors.initials: G
authors.lastname: Zerek-Melen
authors.forename: E
authors.initials: E
authors.lastname: Sewerynek
authors.forename: M
authors.initials: M
authors.lastname: Szkudlinski
authors.forename: A
authors.initials: A
authors.lastname: Lewinski
authors.forename: M
authors.initials: M
authors.lastname: Krotewicz
authors.forename: J
authors.initials: J
authors.lastname: Fryczak
authors.forename: M
authors.initials: M
authors.lastname: Pawlikowski
mesh_terms: D000818:Animals; D003543:Cysteamine / Q000494:pharmacology*; D008297:Male; D008938:Mitosis / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains; D013961:Thyroid Gland / Q000166:cytology / Q000187:drug effects*; D013972:Thyrotropin / Q000037:antagonists & inhibitors* / Q000494:pharmacology
chemical_list: D003543:Cysteamine; D013972:Thyrotropin