Macrophages and dendritic cells during the early stages of antigen-induced arthritis in rats: immunohistochemical analysis of cryostat sections of the whole knee joint.
The appearance of different macrophage subpopulations, Ia-positive antigen-presenting dendritic cells and of T and B lymphocytes was studied in early phases of antigen-induced arthritis in rat knee joints. Cryostat sections of whole knee joints were analysed with immunohistochemical techniques using monoclonal antibodies against rat macrophages, Ia-antigen, and lymphocyte subpopulations. The results showed that in the early phases of the development of arthritis, the synovium was already infiltrated by many monocytes, young macrophages, granulocytes, perivascular Ia-positive non-lymphoid cells, some mature tissue macrophages, and only few T lymphocytes. In later phases not only monocytes, young macrophages and Ia-positive cells became more prominent but also the more mature ED2 positive macrophages and the ED3 positive macrophages that are normally confined to lymphoid organs became increasingly important. The T-cell population increased to some extent in later phases of arthritis induction, possibly induced by clustering with the Ia-positive cells.
Verschure
P J
PJ
Department of Histology, Vrije Universiteit, Amsterdam, The Netherlands.
Van Noorden
C J
CJ
Dijkstra
C D
CD
D000818:Animals; D001168:Arthritis / Q000473:pathology*; D001169:Arthritis, Experimental / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D002465:Cell Movement; D003713:Dendritic Cells / Q000032:analysis / Q000473:pathology*; D005260:Female; D005629:Frozen Sections; D007150:Immunohistochemistry; D007719:Knee Joint / Q000032:analysis / Q000473:pathology*; D008214:Lymphocytes / Q000032:analysis / Q000145:classification; D008264:Macrophages / Q000032:analysis / Q000473:pathology*; D010641:Phenotype; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors