title: Linear chromosome of Borrelia burgdorferi.
abstract: The DNA organization of several European and American isolates of Borrelia burgdorferi, the aetiological agent of Lyme disease, was analysed in pulse-field agarose gel electrophoresis. The results of in situ cell lysis in agarose plugs demonstrated a unique arrangement for the DNA of this spirochete. The chromosome of Borrelia behaved as a eukaryotic linear chromosome with a size of around 1,000 kb. The genome also comprised several circular and linear plasmids which varied in size from 15 to 60 kb.
authors.affiliation: Unité des Leptospires, Institut Pasteur, Paris.
authors.forename: C
authors.initials: C
authors.lastname: Baril
authors.forename: C
authors.initials: C
authors.lastname: Richaud
authors.forename: G
authors.initials: G
authors.lastname: Baranton
authors.forename: I S
authors.initials: IS
authors.lastname: Saint Girons
mesh_terms: D015748:Borrelia burgdorferi Group / Q000235:genetics*; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D066298:In Vitro Techniques; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D010957:Plasmids / Q000235:genetics; D015183:Restriction Mapping
chemical_list: D004269:DNA, Bacterial