title: An inexpensive small volume equilibrium dialysis system for protein-ligand binding assays.
abstract: A simple and inexpensive system for equilibrium dialysis-based binding assays has been developed employing 1.5-ml microtubes. The main advantages are small volume of test buffers, no time-consuming assembly of the test vials, and inexpensive test cells. A comparison of the results derived from this equilibrium dialysis system with those of polyethylenimine-filtration assay and those of a commercially available equilibrium dialysis test system (Diachema from Dianorm) shows good agreement.
authors.affiliation: Institut für Genetik der Universität Bonn, Federal Republic of Germany.
authors.forename: T
authors.initials: T
authors.lastname: Reinard
authors.forename: H J
authors.initials: HJ
authors.lastname: Jacobsen
mesh_terms: D003956:Dialysis / Q000295:instrumentation / Q000379:methods; D007700:Kinetics; D008024:Ligands; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D013997:Time Factors
chemical_list: D008024:Ligands; D011506:Proteins