Monoclonal antibody raised against tetrodonic acid, a derivative of tetrodotoxin.
Tetrodonic acid, a relatively non-toxic derivative of tetrodotoxin, was conjugated with bovine serum albumin and injected i.p. to BALB/c mice. After several injections, spleen cells were isolated, fused with myeloma cells X63-Ag8-6.5.3. and cloned by the limiting dilution method. The monoclonal antibody produced in ascites fluid in the mouse by the cloned cell showed an increasing reactivity with tetrodotoxin at concentrations ranging from 0.03 to 100 micrograms per well.
Watabe
S
S
Department of Fisheries, Faculty of Agriculture, University of Tokyo, Japan.
Sato
Y
Y
Nakaya
M
M
Hashimoto
K
K
Enomoto
A
A
Kaminogawa
S
S
Yamauchi
K
K
D000818:Animals; D000911:Antibodies, Monoclonal; D001001:Anura; D002460:Cell Line; D005260:Female; D008099:Liver / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D011799:Quinazolines / Q000276:immunology*; D013779:Tetrodotoxin / Q000276:immunology*
D000911:Antibodies, Monoclonal; D011799:Quinazolines; C059207:tetrodonic acid; D013779:Tetrodotoxin