[Viability of human red blood cells preserved for 35 days after leukocyte depletion (in vitro study)].
24 leukocyte poor red cells concentrates (L.P.R.C.) were prepared by sterile connection of a leucocyte filter between the primary bag and the SAGM bag of a blood unit after centrifugation. Their quality was followed up to 42 days by means of a panel of tests including, ATP and 2,3-DPG levels, hemolysis, plasma potassium, lactate and glucose, and counts of the microaggregates. 24 standard units acted as a control group. Results showed better preservation of LPRC and especially less hemolysis, higher ATP levels and at least equal oxyphoric capacity (explored by 2,3-DPG). Microaggregate formation was dramatically reduced and bacteriologic checks (48 at day 25 and 48 at day 42) were all negative. Leucocyte depletion appears as a new way to improve functionality of erythrocytes during storage in the SAGM medium. 35 days shelf life will allow this blood product to be more available and its preparation more standardised.
Angué
M
M
Centre Régional de Transfusion Sanguine de Dijon.
Chatelain
P
P
Fiabane
S
S
Domy
M
M
Guignier
F
F
Richaud
P
P
D019794:2,3-Diphosphoglycerate; D000255:Adenosine Triphosphate / Q000097:blood; D001781:Blood Component Removal / Q000379:methods*; D001786:Blood Glucose / Q000032:analysis; D001793:Blood Preservation / Q000379:methods*; D004163:Diphosphoglyceric Acids / Q000097:blood; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000032:analysis; D006461:Hemolysis; D006801:Humans; D007773:Lactates / Q000097:blood; D019344:Lactic Acid; D007962:Leukocytes; D011188:Potassium / Q000097:blood
D001786:Blood Glucose; D004163:Diphosphoglyceric Acids; D007773:Lactates; D019794:2,3-Diphosphoglycerate; D019344:Lactic Acid; D000255:Adenosine Triphosphate; D011188:Potassium
Viabilité des globules rouges humains conservés pendant 35 jours après déplétion en leucocytes (étude in vitro).