title: Predominant localization of phosphoenolpyruvate carboxykinase mRNA in the periportal zone of rat liver parenchyma demonstrated by in situ hybridization.
abstract: In rat liver parenchyma, expression of the phosphoenolpyruvate carboxykinase (PEPCK) gene was studied by Northern blot analysis with a biotinylated cRNA probe and the zonal localization of PEPCK mRNA was demonstrated by in situ hybridization with a radiolabelled cRNA probe. During the feeding period at night, overall PEPCK mRNA levels were low and PEPCK mRNA was detected only in small areas of the periportal zone. At the beginning of the light period (7 am) the overall PEPCK mRNA level began increasing and the periportal areas containing PEPCK mRNA broadened. The maximum of the total abundance and of the area with high levels of PEPCK mRNA was reached at noon. Fasting for 24-72 h did not cause further significant alterations in the level or localization of PEPCK mRNA. The present data are in line with previous findings of the predominant localization of PEPCK activity and enzyme protein in periportal hepatocytes. They suggest that the heterogeneous expression of the PEPCK gene in rat liver is regulated at the pretranslational level.
authors.affiliation: Institut für Biochemie, Georg-August-Universität, Göttingen, FRG.
authors.forename: H
authors.initials: H
authors.lastname: Bartels
authors.forename: H
authors.initials: H
authors.lastname: Linnemann
authors.forename: K
authors.initials: K
authors.lastname: Jungermann
mesh_terms: D000818:Animals; D001665:Binding Sites; D004435:Eating; D005786:Gene Expression Regulation; D006651:Histocytochemistry; D008027:Light; D008099:Liver / Q000201:enzymology*; D008297:Male; D009693:Nucleic Acid Hybridization; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000235:genetics*; D015347:RNA Probes; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
chemical_list: D015347:RNA Probes; D012333:RNA, Messenger; D010729:Phosphoenolpyruvate Carboxykinase (GTP)