Ultrastructural localization of antigenic substances in Trichinella spiralis.
The in situ localization of antigenic substances in Trichinella spiralis muscle larvae was demonstrated at the subcellular level. Larvae recovered from mouse muscle were fixed with half-strength Karnovsky fixative, dehydrated with alcohol, and embedded in LR White resin. Ultrathin sections were incubated with sera from infected Wistar rats and, subsequently, protein A-gold complex. The specificity of the immunostaining was confirmed by a control experiment. Positively immunostaining structures included the stichocyte granules, body cuticle, hindgut cuticle, hypodermis, hemolymph, glycogen aggregates, esophagus-occupying substance (EOS), midgut-occupying substance (MOS), brush border, cytoplasmic granules in the cord, intestinal gland cell granules, and discrete areas in the genital primordial cell. However, the esophageal cuticle, nucleus, nucleolus, mitochondria, rough endoplasmic reticulum, and muscle fibers were negative by immunostaining.
Takahashi
Y
Y
Department of Parasitology, Nara Medical University, Japan.
Uno
T
T
Mizuno
N
N
Yamada
S
S
Araki
T
T
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis*; D003594:Cytoplasmic Granules / Q000276:immunology; D006003:Glycogen / Q000032:analysis; D006458:Hemolymph / Q000276:immunology; D007106:Immune Sera / Q000276:immunology; D007150:Immunohistochemistry; D051379:Mice; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains; D014234:Trichinella / Q000276:immunology* / Q000648:ultrastructure
D000947:Antigens, Helminth; D007106:Immune Sera; D006003:Glycogen