title: Effect of electric field switching on the electrophoretic mobility of single-stranded DNA molecules in polyacrylamide gels.
abstract: We have examined the effects of pulsed electric fields on the separation of single-stranded DNA molecules in polyacrylamide sequencing gels. Using different electric field pulsing regimens, the mobilities of single-stranded DNA molecules can be retarded or increased as compared to conventional electrophoresis. These results indicated that pulsed field techniques can be applied to gel electrophoresis of small single-stranded DNA molecules.
authors.affiliation: Division of Biology, California Institute of Technology, Pasadena 91125.
authors.forename: E
authors.initials: E
authors.lastname: Lai
authors.forename: N A
authors.initials: NA
authors.lastname: Davi
authors.forename: L E
authors.initials: LE
authors.lastname: Hood
mesh_terms: D004277:DNA, Single-Stranded / Q000032:analysis*; D004574:Electromagnetic Fields; D055590:Electromagnetic Phenomena; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*
chemical_list: D004277:DNA, Single-Stranded