Carnivora: the primary structure of the Pacific Walrus (Odobenus rosmarus divergens, Pinnipedia) hemoglobin.
The primary structure of the alpha- and beta-chains of the hemoglobin from the Pacific Walrus (Odobenus rosmarus divergens, Pinnipedia) is presented. Sequence analysis revealed only one hemoglobin component whereas two bands were found in polyacrylamide gel electrophoresis. The globin chains were separated by high-performance liquid chromatography and the sequences determined by automatic liquid- and gas-phase sequencing of the chains and their tryptic peptides. The alpha-chains show 20 and the beta-chains 12 exchanges compared to the corresponding human chains. In the alpha-chains one heme- and two alpha 1/beta 1-contacts were exchanged whereas in the beta-chains one alpha 1/beta 1-, one alpha 1/beta 2-and one heme-contact are substituted. Compared to Harbour Seal (Phoca vitulina) the Walrus hemoglobin shows 9 amino-acid replacements in the alpha-chains and 5 in the beta-chains. The relation between Pinnipedia and Arctoidea is discussed.
Lin
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HX
Max-Planck-Institut für Biochemie, Abteilung Proteinchemie, Martinsried bei München.
Kleinschmidt
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T
Johnson
M L
ML
Braunitzer
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G
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D010872:Caniformia / Q000097:blood*; D004591:Electrophoresis, Polyacrylamide Gel; D005916:Globulins / Q000032:analysis; D006454:Hemoglobins / Q000032:analysis*; D006868:Hydrolysis; D008297:Male; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis / Q000302:isolation & purification; D013045:Species Specificity; D014357:Trypsin; D014856:Walruses / Q000097:blood*
D000596:Amino Acids; D005916:Globulins; D006454:Hemoglobins; D010455:Peptides; D014357:Trypsin