[Antinuclear and anticytoplasmic antibodies in 24 cases of dermatomyositis. Value of western blotting].
Antinuclear and anticytoplasmic antibodies were detected, using 4 methods, in 96% of the sera (23/24) from 24 dermato- or polymyositis patients, who were followed in the Rheumatology Department. Immunofluorescent (IF) labeling of Hep-2 cell smears was more sensitive than IF staining of liver sections (72 vs 67%), and the patterns observed were in agreement 20/24 times. Gelose precipitation is even less sensitive (29%), but enables a characterization of the antigens recognized: 3 anti-RNP, 3 anti-J01, 1 anti-PMScl, 1 anti-SSB and 1 anti-Ro; the latter two specificities were associated with a sicca syndrome. Western-blotting was the most informative method because it was highly sensitive (79%) and identified the principle antigen-antibody systems: anti-U1-RNP (33%) and anti-Scl70 (33%), both associated with myositis with an overlap syndrome (p less than 0.02); anti-J01 (25%) associated with various forms of myositis; and, more rarely, anti-SSB and anti-Ro (both 4%) when a sicca syndrome was present. Finally, non-identified specificities were observed in 37% of the cases.
Meyer
O
O
Laboratoire d'Immuno-Rhumatologie, Hôpital Lariboisière, Paris.
Aeschlimann
A
A
Bourgeois
P
P
Belmatoug
N
N
Palazzo
E
E
Haim
T
T
Kahn
M F
MF
D000293:Adolescent; D000328:Adult; D000368:Aged; D000974:Antibodies, Antinuclear / Q000032:analysis; D001323:Autoantibodies / Q000032:analysis*; D015153:Blotting, Western; D003240:Connective Tissue Diseases / Q000150:complications / Q000276:immunology; D003593:Cytoplasm / Q000276:immunology*; D003882:Dermatomyositis / Q000150:complications / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D008875:Middle Aged; D011233:Precipitin Tests
D000974:Antibodies, Antinuclear; D001323:Autoantibodies
Anticorps antinucléaires et anticytoplasme au cours de 24 dermatopolymyosites. Intérêt du western-blot (immuno-empreinte).