A bile alcohol sulfate as a major component in the bile of the small skate (Raja erinacea).
The nature of bile alcohols and bile acids in gall-bladder and hepatic bile from perfused livers of the small skate (Raja erinacea) has been investigated. The main bile alcohol sulfate was isolated by thin-layer chromatography and analyzed by fast atom bombardment mass spectrometry and 13C NMR. Following solvolysis and purification on Lipidex-DEAP, the bile alcohol profile was measured by capillary gas-liquid chromatography-electron impact mass spectrometry. Based on these studies and on comparison with authentic scymmnol sulfate and scymnol, the main bile alcohol was identified as 5 beta-cholestane-3 alpha,7 alpha,12 alpha,24 xi,26,27-hexol sulfate. The mean +/- SD concentration in gallbladder bile from five different skates was 24.6 +/- 8.7 mmol/l. Only 0.1 mmol/l of cholic acid and its conjugates was found in a pool of skate bile. The main bile alcohol sulfate in the bile of the small skate seems to be a metabolic end product, present in a concentration comparable to that of bile salts in mammals.
Karlaganis
G
G
Department of Clinical Pharmacology, University of Berne, Switzerland.
Bradley
S E
SE
Boyer
J L
JL
Batta
A K
AK
Salen
G
G
Egestad
B
B
Sjövall
J
J
D000818:Animals; D001646:Bile / Q000032:analysis*; D001653:Bile Ducts, Intrahepatic; D002777:Cholestanols / Q000032:analysis*; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D004555:Electric Fish / Q000378:metabolism*; D005704:Gallbladder; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D012860:Skates, Fish / Q000378:metabolism*
D002777:Cholestanols; C053278:cholestane-3,7,12,24,25,26-hexol