Identification of a novel 80 kDa antigen associated with cell proliferation.
Exploiting the immunomasking method, a polyclonal antibody has been developed in mice for identification of a novel 80 kDa antigen (P80) in KB cells. The P80 was not detected in normal resting cells but was present in appreciable amount in malignant cells. In a comparative Western transfer the antisera to the immune complexes identified a 80 kDa peptide absent in normal cell extracts. When growth of KB cells were arrested by 48 h of serum starvation the P80 was not detected but after refeeding with serum containing medium, the P80 reappeared within 1 h. This result indicates that the P80 is associated with cell proliferation and appears early in the GI-S phase of the cell cycle.
Chatterjee
A
A
Chittaranjan National Cancer Research Centre, Calcutta, India.
Datta
K
K
Roychowdhury
J
J
D000936:Antigen-Antibody Complex / Q000276:immunology; D000951:Antigens, Neoplasm / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis; D015153:Blotting, Western; D002455:Cell Division; D002460:Cell Line; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D009363:Neoplasm Proteins / Q000032:analysis*
D000936:Antigen-Antibody Complex; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D003470:Culture Media; D007106:Immune Sera; D009363:Neoplasm Proteins; C036987:protein p80