In vitro experiments on neuronal and glial cell lineages among the ventricular cells of the mouse neural plate.
The proliferative ventricular cells of the early neural plate of the mouse are generally assumed to be pluripotent and equivalent to one another in their developmental capability. Ventricular cells from the rostral parts of the neural plates of mice (Theiler stages 11 and 12, embryonic days 71/2 and 8) were studied in tissue culture with respect to their potential to give rise to neurons or glial cells, or both. Autoradiographic and immunohistochemical analyses showed that ventricular cells developing into neuronal phenotypes stopped proliferating immediately upon transfer to cell culture. Using polyclonal anti-GFAP antibodies, a small proportion of immunoreactive cells could be detected after 4 days of culture. These cells retained their proliferative activity, displayed morphological characteristics of radial glial cells, and may have either developed from specific glial progenitor cells or have been induced to proceed along the glial differentiation pathway at the beginning of culture. Therefore, two distinct types of progenitor cells, committed either to neuronal or glial lineages, appear to co-exist among the cultured neural plate ventricular cells.
Buse
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Anatomisches Institut, Universität Kiel, F.R.G.
Kindler-Röhrborn
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A
Rajewsky
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MF
D000818:Animals; D000911:Antibodies, Monoclonal; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D002454:Cell Differentiation; D002455:Cell Division; D002552:Cerebral Ventricles / Q000166:cytology / Q000196:embryology*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D013936:Thymidine
D000911:Antibodies, Monoclonal; D005904:Glial Fibrillary Acidic Protein; D013936:Thymidine