Autoradiographic study of tritium-labeled misonidazole in the mouse.
The localization of tritiated misonidazole metabolites in a number of normal tissues in the mouse is reported from autoradiography. The labeled misonidazole was injected at 750 or 75 mg/kg body weight (Rel. Sp. Act. 74 and 740 MBq/mg respectively). The grain count ratio, parenchyma:stroma, for selected tissues was: liver (centrilobular zone) 13; meibomian gland (acini) 68, (duct) 116; esophagus (keratinized layer) 61; enamel organ 17. It is concluded that there are a number of tissues which will accumulate MISO metabolites although they may not all be hypoxic.
Cobb
L M
LM
Division of Experimental Pathology and Therapeutics, MRC Radiobiology Unit, Didcot, Oxon, United Kingdom.
Nolan
J
J
D000818:Animals; D001345:Autoradiography; D004658:Enamel Organ / Q000378:metabolism; D004947:Esophagus / Q000378:metabolism; D005260:Female; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008808:Mice, Inbred CBA; D008920:Misonidazole / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D014316:Tritium
D014316:Tritium; D008920:Misonidazole; D010100:Oxygen