A simple method for the characterization of photoproducts from DNA applied to the cis-syn thymine dimer.
A method for the unambiguous characterization of DNA photoproducts has been developed. It does not require radio-labelled DNA, or specialized techniques. In the preliminary step, UV-irradiated DNA is hydrolysed to its constituent bases and photoproducts. The photoproducts are then separated from nucleic acid bases using low pressure ion-exchange chromatography on a Dowex 1 x 8 200-400, and an Amberlite CG-50-H+ column. Further separation and purification of photoproducts is carried out on the HPLC Whatman Partisil ODS2 column, using 15% MeOH. The procedure is simple, reproducible and versatile.
Salter
L F
LF
Department of Chemistry and Applied Chemistry, University of Natal, Durban, Republic of South Africa.
Thomas
R C
RC
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000032:analysis*; D010777:Photochemistry; D013941:Thymine / Q000032:analysis*; D013950:Thymus Gland / Q000032:analysis
D004247:DNA; D013941:Thymine