Synthesis of the dodecapeptide corresponding to domain III of bovine brain calmodulin: alpha-beta shift side reactions during the synthesis by the classical method in solution.
We report details of the chemical synthesis of the dodecapeptide corresponding to the calcium binding loop III of bovine brain calmodulin (sequence 93-104) and its fragments 96-04, 93-98, and 99-104. The preparation of the peptides employed classical solution methods and a fragment-condensation strategy. The major difficulties were encountered during the synthesis of the peptides containing the N-terminal sequences -Gly-Asn-Gly- and -Asp-Lys-Asp-Gly-Ans-Gly-, in which alpha-beta shift side reactions were observed.
Borin
G
G
Calderan
A
A
Ruzza
P
P
Pezzoli
A
A
Marchiori
F
F
Peggion
E
E
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism; D002147:Calmodulin / Q000138:chemical synthesis*; D002417:Cattle; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis*
D002147:Calmodulin; D007202:Indicators and Reagents; D010455:Peptides