Electrophysiological properties of neurons in neonatal rat occipital cortex slices grown in a serum-free medium.
The electrophysiological properties of individual neurons within organotypic explants of neonatal rat cortex were examined via intracellular recordings. The explants were grown for two weeks in a serum-free medium. The electrophysiological properties of the neurons within these explants were similar to those reported for both adult cortex in vivo and short-term in vitro slice preparations. The results of the present study show that cortical explants grown under serum-free conditions can serve as a useful model for long-term developmental studies associated with the physiological basis of neural network formation.
Baker
R E
RE
The Netherlands Institute for Brain Research, Amsterdam.
Bingmann
D
D
Ruijter
J M
JM
D000200:Action Potentials; D000818:Animals; D003470:Culture Media; D046508:Culture Techniques; D008564:Membrane Potentials; D009474:Neurons / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D014793:Visual Cortex / Q000166:cytology / Q000502:physiology*
D003470:Culture Media