title: Chemical modification and composition of tetrameric isozyme K of alkaline phosphatase from harp seal intestinal mucosa.
abstract: 1. The carbohydrate content of isozyme K of alkaline phosphatase (EC 3.1.3.1) from harp seal intestinal mucosa was examined. The presence of N-acetylglucosamine, N-acetylgalactosamine and considerable amounts of mannose residues was shown. 2. The amino acid content of seal alkaline phosphatase was determined. A high extent of homology (85%) between bovine and seal alkaline phosphatases was demonstrated. 3. By chemical modification lysine, dicarboxylic acids, arginine and tyrosine residues of tetrameric seal alkaline phosphatase are located near or at the active site. By contrast, the modification of either thiol or imidazole groups resulted in no alterations of the enzyme activity. 4. It has been demonstrated that inorganic phosphate is an inhibitor of alkaline phosphatase and entirely prevents the enzyme inactivation with succinic anhydride.
authors.affiliation: Laboratory of Biologically Active Substances of Hydrobionts, Ministry of Health, Moscow, USSR.
authors.lastname: Sakharov IYu
authors.forename: I E
authors.initials: IE
authors.lastname: Makarova
authors.forename: G A
authors.initials: GA
authors.lastname: Ermolin
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis* / Q000037:antagonists & inhibitors; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D010872:Caniformia / Q000378:metabolism*; D002241:Carbohydrates / Q000032:analysis; D007413:Intestinal Mucosa / Q000201:enzymology; D007527:Isoenzymes / Q000032:analysis* / Q000037:antagonists & inhibitors; D007700:Kinetics; D010710:Phosphates / Q000494:pharmacology; D011487:Protein Conformation; D046048:Seals, Earless / Q000378:metabolism*; D013387:Succinic Anhydrides / Q000494:pharmacology
chemical_list: D000596:Amino Acids; D002241:Carbohydrates; D007527:Isoenzymes; D010710:Phosphates; D013387:Succinic Anhydrides; C031801:succinic anhydride; D000469:Alkaline Phosphatase