title: Binding of bullous pemphigoid and pemphigus vulgaris sera to SCaBER cell line in an ELISA.
abstract: Specific antibodies present in the sera of patients with bullous pemphigoid or pemphigus vulgaris were detected in an enzyme-linked immunosorbent assay (ELISA) employing a squamous carcinoma cell line, SCaBER, as substrate. Bullous pemphigoid sera bound preferentially to permeabilized cells, suggesting that the antigens are largely intracellular. The assay may prove to be a useful addition to current methods of detecting circulating antibodies in these patients.
authors.affiliation: Dermatology Research Unit E5, Addenbrooke's Hospital, Cambridge, U.K.
authors.forename: A L
authors.initials: AL
authors.lastname: Cook
authors.forename: R B
authors.initials: RB
authors.lastname: Mallett
authors.forename: T H
authors.initials: TH
authors.lastname: Hanahoe
authors.forename: R J
authors.initials: RJ
authors.lastname: Pye
mesh_terms: D000937:Antigen-Antibody Reactions; D001323:Autoantibodies / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000276:immunology*; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D010391:Pemphigoid, Bullous / Q000097:blood / Q000276:immunology*; D010392:Pemphigus / Q000097:blood / Q000276:immunology*; D012872:Skin Diseases, Vesiculobullous / Q000276:immunology*; D001749:Urinary Bladder Neoplasms / Q000276:immunology
chemical_list: D001323:Autoantibodies