Molecular characterization of the gene encoding SHV-3 beta-lactamase responsible for transferable cefotaxime resistance in clinical isolates of Klebsiella pneumoniae.
In Klebsiella pneumoniae 86-4, cefotaxime resistance was due to a transferable broad-spectrum beta-lactamase, SHV-3. The plasmid-borne gene encoding SHV-3 has been cloned, and the primary structure of the enzyme was deduced from its nucleotide sequence. SHV-3 differs from SHV-1 in two positions. The extended substrate profile of SHV-3 probably results from the substitution of Ser-213 for Gly, as in SHV-2, whereas replacement of Arg-180 by Leu resulted in a decrease in the pI from 7.6 to 7.0. The blashv-3 gene is highly homologous (92% DNA sequence identity) with the chromosomal gene coding for LEN-1 beta-lactamase of K. pneumoniae, suggesting that the origin of the SHV-encoding genes now present on many plasmids may be chromosomal.
Nicolas
M H
MH
Laboratoire de Génétique Moléculaire Bactérienne, Institut Pasteur, Paris, France.
Jarlier
V
V
Honore
N
N
Philippon
A
A
Cole
S T
ST
D001483:Base Sequence; D002439:Cefotaxime / Q000494:pharmacology*; D003001:Cloning, Molecular; D003428:Cross Infection / Q000382:microbiology; D003470:Culture Media; D004352:Drug Resistance, Microbial / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D007710:Klebsiella Infections / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids; D015183:Restriction Mapping; D001618:beta-Lactamases / Q000235:genetics*
D003470:Culture Media; C062555:beta-lactamase SHV-3; D001618:beta-Lactamases; D002439:Cefotaxime