[A method of solid phase immunoenzyme analysis using antigen molecules immobilized on membranes].
The enzyme-linked immunoassay modification has been worked out. The method combines advantages of membrane technology of antigen immobilization which is used in the enzyme immunosensory technique and of conventional enzyme-linked immunosorbent assay. The nitrocellulose and polypropylene membranes are used as a solid-phase. The purified rabbit immunoglobulin G is immobilized on the surface of membranes as the first layer. The competitive immunoassay is employed. The immunoglobulin G concentration range is 1-1000 ng/ml. The membranes with the immobilized antigen can be repeatedly used after incubation in 0.1 M glycine buffer, pH 2.5. The dry membrane with the immobilized antigen can be used after keeping for 6 months in refrigerator at 4 degrees C without changing the concentration range measured.
Arenkov
P Ia
PIa
Zhmareva
E N
EN
Berezin
V A
VA
D000818:Animals; D000941:Antigens / Q000032:analysis*; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis; D008567:Membranes, Artificial; D011817:Rabbits
D000941:Antigens; D007074:Immunoglobulin G; D008567:Membranes, Artificial
Metod tverdofaznogo immunofermentnogo analiza s ispol'zovaniem immobilizovannykh na membranakh molekul antigena.