[The effect of histological processing on the binding of lectins].
Binding of FITC labelled lectins of LCA, PNA, and HPA in fundus, jejunum, liver and kidney of rabbit, guinea pig, rat and mouse had been studied with the use of three different methods of histological processing of the organs. The findings in cryocut postfixed sections, shortly dehydrated and in paraffin embedded samples and with common paraffin technique treated organs were compared. Results demonstrate that the influence of compared histological methods on lectin binding is not univocal. In some cases (e.g. fundus, rabbit, LCA and PNA) the binding of lectins is not modified by processing of tissue, in other cases (e.g. liver, rabbit, LCA) great differences between cryocut sections and sections of dehydrated and in paraffin embedded material were found. Generally it could be stated that common paraffin technique is not a very suitable method for lectin histochemistry.
Jelínek
F
F
D000818:Animals; D005753:Gastric Mucosa / Q000378:metabolism; D006168:Guinea Pigs; D006652:Histological Techniques; D007583:Jejunum / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D037102:Lectins / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D051379:Mice; D011817:Rabbits; D051381:Rats
D037102:Lectins
Vliv histologického zpracování na vazbu nĕkterých lektinů.