Inhibition of spontaneous growth and induced differentiation of murine erythroleukaemia cells by paraquat and atrazine.
The effect of the herbicides paraquat and atrazine on erythroid differentiation has been studied in mouse erythroleukaemic cells. The addition of atrazine or paraquat was shown to inhibit both spontaneous growth and hexamethylene-bis-acetamide (HMBA)-induced differentiation of undifferentiated erythroleukaemic cells. This effects was dose-dependent and occurred at concentrations of less than 10 ppm for both herbicides. Growth inhibition with atrazine (40-45%) was less pronounced than with paraquat (85-90%). Inhibition of differentiation paralleled growth inhibition. A synergistic effect was observed with HMBA, which per se reduced the growth rate of mouse erythroleukaemic cells, and either herbicide. Evaluation of cell viability under all the experimental conditions using either a trypan blue dye exclusion test or labelled chromium indicated that the effects observed were not related to a cytocidal action of atrazine or paraquat.
Magnelli
L
L
Institute of General Pathology of Florence University, Italy.
Fibbi
G
G
Caldini
R
R
Pucci
M
M
Del Rosso
M
M
D000081:Acetamides; D000818:Animals; D001280:Atrazine / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004920:Erythropoiesis / Q000187:drug effects*; D006131:Growth Inhibitors; D051379:Mice; D010269:Paraquat / Q000633:toxicity*
D000081:Acetamides; D006131:Growth Inhibitors; C014026:hexamethylene bisacetamide; D010269:Paraquat; D001280:Atrazine