title: Delta-sleep-inducing peptide (DSIP) stimulates the release of immunoreactive Met-enkephalin from rat lower brainstem slices in vitro.
abstract: We studied whether delta-sleep-inducing peptide (DSIP) acted on opioid receptor directly or indirectly. DSIP did not have binding activity to any subtype of opioid receptors. DSIP at doses of 1 pM-1 nM significantly stimulated the release of immunoreactive Met-enkephalin (iME) from superfused slices of the rat lower brainstem. The DSIP-induced release of iME was calcium-dependent. These results show that DSIP acts on opioid receptor indirectly by stimulating the release of iME in producing antinociceptive effects.
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, Fukuyama University, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Nakamura
authors.forename: M
authors.initials: M
authors.lastname: Nakashima
authors.forename: K
authors.initials: K
authors.lastname: Sakai
authors.forename: M
authors.initials: M
authors.lastname: Niwa
authors.forename: M
authors.initials: M
authors.lastname: Nozaki
authors.forename: H
authors.initials: H
authors.lastname: Shiomi
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D003701:Delta Sleep-Inducing Peptide / Q000494:pharmacology*; D004174:Diprenorphine / Q000378:metabolism; D004723:Endorphins / Q000378:metabolism; D004744:Enkephalin, Methionine / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D008526:Medulla Oblongata / Q000187:drug effects / Q000378:metabolism*; D011149:Pons / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D004723:Endorphins; D004174:Diprenorphine; D004744:Enkephalin, Methionine; D003701:Delta Sleep-Inducing Peptide