Enzymatic deglycosylation of the dendrotoxin-binding protein.
The neuronal membrane protein which binds the K+-channel ligands dendrotoxin, mast cell degranulating peptide, and beta-bungarotoxin was purified from rat brain membranes. When analysed on 10% SDS gel electrophoresis, the purified protein contained two peptides: the toxin-binding subunit of apparent Mr 90,000 and another peptide of Mr 38,000. Neuraminidase treatment reduced the Mr of the toxin-binding subunit to 70,000. Glycopeptidase F gave a further reduction to Mr 65,000. In contrast, the peptide of Mr 38,000 showed no change in Mr upon treatment with neuraminidase and/or glycopeptidase F. It is concluded that the toxin-binding subunit of the dendrotoxin-binding protein, a presumptive K+ channel, is a sialated membrane protein with a peptide core of, at most, Mr 65,000.
Rehm
H
H
VA Medical Center, GRECC 182-B, Seattle, WA 98108.
D000581:Amidohydrolases / Q000378:metabolism*; D000818:Animals; D001514:Bee Venoms; D001923:Brain Chemistry; D002038:Bungarotoxins / Q000378:metabolism; D002462:Cell Membrane / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006031:Glycosylation; D008970:Molecular Weight; D009439:Neuraminidase / Q000378:metabolism*; D009498:Neurotoxins; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D010455:Peptides / Q000378:metabolism; D010766:Phosphorylation; D015221:Potassium Channels / Q000378:metabolism; D051381:Rats; D011950:Receptors, Cholinergic / Q000302:isolation & purification / Q000378:metabolism*
D001514:Bee Venoms; D002038:Bungarotoxins; D009498:Neurotoxins; D010455:Peptides; D015221:Potassium Channels; D011950:Receptors, Cholinergic; C042467:dendrotoxin receptor; C008331:mast cell degranulating peptide; D009439:Neuraminidase; D000581:Amidohydrolases; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase