Antibodies to nystatin demonstrate polyene sterol specificity and allow immunolabeling of sterols in Saccharomyces cerevisiae.
Polyclonal antibodies elicited by injection into rabbits of a nystatin-bovine serum albumin conjugate were reactive with both nystatin and amphotericin B. Upon labeling of polyene-treated Saccharomyces cerevisiae sterol auxotrophs grown on various sterols, nystatin reacted specifically with ergosterol, while amphotericin B did not react preferentially with ergosterol, cholesterol, or cholestanol. Time course labeling experiments demonstrated the rate of ergosterol transport into cholesterol-grown cells.
Walker-Caprioglio
H M
HM
Department of Microbiology, North Carolina State University, Raleigh 27695-7615.
MacKenzie
J M
JM
Parks
L W
LW
D000818:Animals; D000918:Antibody Specificity; D001711:Biotransformation; D004797:Enzyme-Linked Immunosorbent Assay; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D009761:Nystatin / Q000276:immunology*; D011090:Polyenes / Q000276:immunology; D011817:Rabbits; D012441:Saccharomyces cerevisiae / Q000032:analysis* / Q000276:immunology; D013056:Spectrophotometry, Ultraviolet; D013261:Sterols / Q000032:analysis / Q000276:immunology*
D011090:Polyenes; D013261:Sterols; D009761:Nystatin