title: NAD-linked, GSH- and factor-independent aldehyde dehydrogenase of the methylotrophic bacterium, Hyphomicrobium X.
abstract: Cell-free extracts of Hyphomicrobium X showed NAD-dependent aldehyde dehydrogenase activity, provided that NAD addition preceded that of aldehyde. Activity was lost rather rapidly, especially during purification attempts, but this could be partially masked by including a time-dependent restoration step with thiol compounds in the protocol. The nature of the assay buffer appeared to be critical and stimulation occurred on incorporation of K+ ions in the mixture. An even higher specific activity could be achieved by 1,4-dithiothreitol (DTT) treatment of the preparation, followed by removal of DTT, and assaying in the absence of thiol compounds under anaerobic conditions. Exposure of such a preparation to O2 led to a significant decrease in activity within a couple of hours. Immediate inactivation occurred on addition of H2O2, but this could be prevented completely by prior addition of NAD. Since GSH does not participate in the reaction and no stimulating factor was detected, the role of thiol compounds is most probably confined to restoration or prevention of damage to an O2-sensitive, necessary thiol group. Since the same features were found for cell-free extract as for the partially purified enzyme, only one enzyme type seems to be present. Although the enzyme is a general aldehyde dehydrogenase, the kinetic parameters and the specific activity of the cell-free extract for formaldehyde indicate that it may play a role in formaldehyde dissimilation by Hyphomicrobium X. The NAD-linked, GSH- and factor-independent aldehyde dehydrogenase described here appears to be different in several respects from the formaldehyde dehydrogenase of Pseudomonas putida (EC 1.2.1.46) (despite showing similar behavior toward coenzymes and factors) but resembles the aldehyde dehydrogenase from baker's yeast (EC 1.2.1.5).
authors.affiliation: Department of Microbiology and Enzymology, Delft University of Technology, The Netherlands.
authors.forename: P A
authors.initials: PA
authors.lastname: Poels
authors.forename: J A
authors.initials: JA
authors.lastname: Duine
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D001419:Bacteria / Q000201:enzymology*; D002474:Cell-Free System; D004789:Enzyme Activation / Q000187:drug effects; D005557:Formaldehyde / Q000378:metabolism; D005978:Glutathione / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D009243:NAD / Q000494:pharmacology*; D011188:Potassium / Q000494:pharmacology; D013379:Substrate Specificity
chemical_list: D009243:NAD; D005557:Formaldehyde; D006861:Hydrogen Peroxide; D000444:Aldehyde Dehydrogenase; D005978:Glutathione; D011188:Potassium