[Relation between the damaging effect of surface-active compounds on Escherichia coli cells and the phase of culture growth].
The techniques of cell electrophoresis and electro-orientation spectroscopy were used to study the effect of sodium dodecyl sulfate (SDS) and cetyl trimethyl ammonium bromide (CTAB) on Escherichia coli K-12 cells from the culture at the exponential and stationary growth phases. SDS (2 x 10(-4) M) considerably damaged cells at the exponential phase, particularly at pH less than 6.0, whereas cells at the stationary phase were damaged to a less degree and only at pH less than 5.3 or after their treatment with Trilon B. The damaging effect of SDS decreased in an isotonic medium (0.25 M sucrose) as compared to a hypotonic medium (distilled water). CTAB also damaged cells at the exponential phase more than those at the stationary phase, and its damaging action decreased with pH. Mg2+, Ca2+, and Sr2+ cations diminished the degree of cell damage with CTAB, but did not exert any noticeable protection in the case of SDS. The different sensitivity of cells at the exponential and stationary growth phases may be associated with changes in their surface electric charge and with the existence of hydrophobic regions on the cell surface. The higher electric charge of cells at the stationary growth phase is presumed to stem from a rise in the amount of surface lipopolysaccharides which bear a negative electric charge.
Ivanov
A Iu
AIu
Fomchenkov
V M
VM
D002021:Buffers; D003470:Culture Media; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D006863:Hydrogen-Ion Concentration; D013501:Surface-Active Agents / Q000494:pharmacology*
D002021:Buffers; D003470:Culture Media; D013501:Surface-Active Agents
Zavisimost' povrezhdaiushchego deĭstviia poverkhnostno-aktivnykh veshchestv na kletki Escherichia coli ot fazy rosta kul'tury.