Presence of tumour necrosis factor or a related factor in human basophil/mast cells.
The observation that mast cell products and cachectin/tumour necrosis factor (TNF) mediate similar responses suggested an investigation of cultured human basophil/mast cells for production of TNF. Using in situ hybridization and the avidin biotin-complex (ABC) immunoperoxidase method, we have demonstrated the presence of TNF mRNA in the cytoplasm and TNF protein in the granules of individual human basophil/mast cells. The production of TNF by these cells could explain many of their reported functions.
Steffen
M
M
Department of Developmental Hematopoiesis, Memorial Sloan-Kettering Cancer Center, New York, New York.
Abboud
M
M
Potter
G K
GK
Yung
Y P
YP
Moore
M A
MA
D001491:Basophils / Q000378:metabolism*; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism; D006801:Humans; D008407:Mast Cells / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D014409:Tumor Necrosis Factor-alpha