Electron transport and electrochemical proton gradient in membrane vesicles of Clostridium thermoautotrophicum.
Membrane vesicles of Clostridium thermoautotrophicum containing carbon monoxide dehydrogenase generated a proton motive force when exposed to CO. This proton motive force, with a value of -140 mV, consisted of only an electrical potential at pH 7.5 and above and of an electrical potential and pH gradient at a lower pH. The proton motive force drove the uptake of L-alanine by the vesicles to a concentration of 300 times that of the medium.
Hugenholtz
J
J
Center for Biological Resource Recovery, University of Georgia, Athens 30602.
Ljungdahl
L G
LG
D000409:Alanine / Q000378:metabolism; D001693:Biological Transport, Active; D002462:Cell Membrane / Q000502:physiology; D002474:Cell-Free System; D003013:Clostridium / Q000502:physiology*; D004579:Electron Transport; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008564:Membrane Potentials
D000409:Alanine