title: Proton nuclear magnetic resonance studies on the variant-3 neurotoxin from Centruroides sculpturatus Ewing: sequential assignment of resonances.
abstract: We report the sequential assignment of resonances to specific residues in the proton nuclear magnetic resonance spectrum of the variant-3 neurotoxin from the scorpion Centruroides sculpturatus Ewing (range southwestern U.S.A.). A combination of two-dimensional NMR experiments such as 2D-COSY, 2D-NOESY, and single- and double-RELAY coherence transfer spectroscopy has been employed on samples of the protein dissolved in D2O and in H2O for assignment purposes. These studies provide a basis for the determination of the solution-phase conformation of this protein and for undertaking detailed structure-function studies of these neurotoxins that modulate the flow of sodium current by binding to the sodium channels of excitable membranes.
authors.affiliation: Comprehensive Cancer Center, University of Alabama, Birmingham 35294.
authors.forename: D G
authors.initials: DG
authors.lastname: Nettesheim
authors.forename: R E
authors.initials: RE
authors.lastname: Klevit
authors.forename: G
authors.initials: G
authors.lastname: Drobny
authors.forename: D D
authors.initials: DD
authors.lastname: Watt
authors.forename: N R
authors.initials: NR
authors.lastname: Krishna
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D009498:Neurotoxins; D011487:Protein Conformation; D012604:Scorpion Venoms / Q000302:isolation & purification
chemical_list: C035413:Centruroides toxin; D009498:Neurotoxins; D012604:Scorpion Venoms