title: Electrophoretic variants of enzymes in isolates of Plasmodium falciparum, P. malariae and P. vivax from Thailand.
abstract: A new electrophoretic variant of glucose phosphate isomerase (GPI), which we now denote GPI-3, has been found in isolates of Plasmodium falciparum from 6 patients, all of whom acquired the infection in the same region (in or near Prachinburi province) of Thailand. In other regions, from which 453 isolates have been tested, only GPI-1 and/or GPI-2 have been found. Two isolates of P. malariae from patients at Kanchanaburi showed a band of GPI activity on cellulose acetate gels at a cathodal position quite distinct from that of any previously known GPI variants in other human malaria parasites. Thirty-nine isolates of P. vivax from 3 regions of Thailand have been examined for variants of GPI and lactate dehydrogenase (LDH). Three forms of GPI were found, corresponding approximately in band positions to GPI-1, 2 and 3 of P. falciparum. The position of the band of LDH activity in P. vivax was the same in all the isolates examined, and different from that of LDH-1 in P. falciparum.
authors.affiliation: Biology Department, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.
authors.forename: S
authors.initials: S
authors.lastname: Thaithong
authors.forename: N
authors.initials: N
authors.lastname: Siripoon
authors.forename: N
authors.initials: N
authors.lastname: Seugorn
authors.forename: D
authors.initials: D
authors.lastname: Bunnag
authors.forename: G H
authors.initials: GH
authors.lastname: Beale
mesh_terms: D000818:Animals; D004588:Electrophoresis, Cellulose Acetate; D005956:Glucose-6-Phosphate Isomerase / Q000032:analysis*; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D010963:Plasmodium falciparum / Q000201:enzymology*; D010965:Plasmodium malariae / Q000201:enzymology*; D010966:Plasmodium vivax / Q000201:enzymology*; D013785:Thailand
chemical_list: D007770:L-Lactate Dehydrogenase; D005956:Glucose-6-Phosphate Isomerase