title: Sequence analysis of operator mutants of the phase-1 flagellin-encoding gene, fliC, in Salmonella typhimurium.
abstract: In phase-2 cells of diphasic Salmonella strains, expression of the phase-1 flagellin-encoding gene, fliC, is repressed by the repressor encoded by the fljA gene. Nine operator-constitutive (Oc) mutants of fliC were isolated from S. typhimurium by selecting those which could express fliC in the presence of the repressor. Among them, eight mutants could express fliC both in the presence and the absence of the repressor, whereas the ninth one could express only in the presence of the repressor. Nucleotide sequence analysis revealed that the Oc mutations of the former type were all located between bp 7 and 20 upstream from the coding region of fliC, which suggests that this region may correspond to the operator for fliC. The latter mutant was found to have a tandem duplication of 28 bp which contains a part of the operator sequence, and seems to require the repressor to activate fliC expression.
authors.affiliation: Department of Biology, School of Education, Waseda University, Tokyo, Japan.
authors.forename: Y H
authors.initials: YH
authors.lastname: Inoue
authors.forename: K
authors.initials: K
authors.lastname: Kutsukake
authors.forename: T
authors.initials: T
authors.lastname: Iino
authors.forename: S
authors.initials: S
authors.lastname: Yamaguchi
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D005408:Flagellin / Q000235:genetics*; D005798:Genes, Bacterial; D005809:Genes, Regulator; D005838:Genotype; D008969:Molecular Sequence Data; D009154:Mutation; D009876:Operon; D015183:Restriction Mapping; D012486:Salmonella typhimurium / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D001426:Bacterial Proteins; D005408:Flagellin