Myocardial salvage with trolox and ascorbic acid for an acute evolving infarction.
Both Trolox (a water-soluble analogue of alpha-tocopherol) and ascorbic acid were more effective than superoxide dismutase or catalase in protecting myocyte cell cultures from free radical attack (induced by hypoxanthine and xanthine oxidase). In a canine model of two hours of left anterior descending coronary artery occlusion followed by four hours of reperfusion, Trolox and ascorbic acid reduced the area of infarction within the area at risk. The Trolox group received 500 mL of deoxygenated saline solution containing 2.0 g of Trolox, 3.0 g of ascorbic acid, and 18 mg of EDTA (ethylenediaminetetraacetic acid) infused into the ascending aorta 30 seconds before and four minutes after reperfusion. Saline controls received 500 mL of deoxygenated saline solution containing 18 mg of EDTA. The angioplasty group had unmodified reperfusion by simple release of the occlusion. The area at risk and the area infarcted were estimated with Evans blue and triphenyl tetrazolium hydrochloride stains, respectively. The ratio of the area infarcted to the area at risk was significantly lower with Trolox (angioplasty, 30.4% +/- 5.1%; saline, 20.8% +/- 2.9%; and Trolox, 8.7% +/- 4.0%; p less than 0.01). In summary, the antioxidants Trolox and ascorbic acid effectively reduced myocardial necrosis after ischemia.
Mickle
D A
DA
Department of Clinical Biochemistry, Toronto General Hospital, University of Toronto, Ontario, Canada.
Li
R K
RK
Weisel
R D
RD
Birnbaum
P L
PL
Wu
T W
TW
Jackowski
G
G
Madonik
M M
MM
Burton
G W
GW
Ingold
K U
KU
D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D001205:Ascorbic Acid / Q000627:therapeutic use*; D001578:Benzopyrans / Q000627:therapeutic use*; D002478:Cells, Cultured; D002839:Chromans / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004285:Dogs; D005609:Free Radicals; D006321:Heart / Q000187:drug effects; D006439:Hemodynamics / Q000187:drug effects; D009203:Myocardial Infarction / Q000188:drug therapy*; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D009206:Myocardium / Q000473:pathology; D009336:Necrosis
D000975:Antioxidants; D001578:Benzopyrans; D002839:Chromans; D005609:Free Radicals; D001205:Ascorbic Acid; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid