Endothelial cell seeding on PTFE vascular prostheses using a standardized seeding technique.
A standardized method was developed for seeding endothelial cells (EC) in tubular vascular grafts. A rotational cell seeding device for tubular prostheses is presented and parameters influencing the kinetics of cell adhesion (rotation speed, graft diameter, cell suspension level, inoculated cell number) are reported. Seeding EC in 14 mm ID PTFE vascular grafts with rotation rate of 10 rph gave an adhesion rate of 80% in a homogeneous monolayer.
Gerlach
J
J
Chirurgische Klinik, Universitätsklinikum RV/Charlottenburg, Freie Universität Berlin, West Germany.
Kreusel
K M
KM
Schauwecker
H H
HH
Bücherl
E S
ES
D001807:Blood Vessel Prosthesis; D002448:Cell Adhesion; D004730:Endothelium, Vascular / Q000166:cytology*; D006083:Graft Occlusion, Vascular / Q000517:prevention & control*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D011138:Polytetrafluoroethylene; D013499:Surface Properties
D011138:Polytetrafluoroethylene