title: [Immunoaffinity purification of alpha 1-protease inhibitor from human plasma].
abstract: A single-step procedure is described for isolation of alpha 1-inhibitor of proteases (alpha 1-IP) from human blood plasma using affinity chromatography on immunosorbent containing monoclonal antibodies against alpha 1-IP. Activity of the alpha 1-IP preparation, estimated by means of specific trypsin inhibition, was increased 61.1-fold after the chromatography, with a yield of the end product 20%. The preparation of alpha 1-IP obtained exhibited electrophoretic and immunochemical homogeneity.
authors.forename: S M
authors.initials: SM
authors.lastname: Podiarene
authors.forename: M N
authors.initials: MN
authors.lastname: Letskene
authors.forename: M M
authors.initials: MM
authors.lastname: Mauritsas
authors.forename: R R
authors.initials: RR
authors.lastname: Planchiunene
mesh_terms: D000911:Antibodies, Monoclonal; D002846:Chromatography, Affinity; D006801:Humans; D007122:Immunoelectrophoresis; D007163:Immunosorbent Techniques; D000515:alpha 1-Antitrypsin / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000911:Antibodies, Monoclonal; D000515:alpha 1-Antitrypsin
vernacular_title: Immunoaffinnaia ochistka alpha 1-ingibitora proteaz iz plazmy krovi cheloveka.