Subnanogram GC/NPD method for the determination of sparteine in biological fluids.
A gas chromatographic method is presented for identification and quantification of sparteine in biological fluids using cyclizine as an internal standard. No derivation is necessary and after a single alkaline extraction, GC analysis of sparteine is achieved in less than 8 min. A subnanogram limit of detection is allowed by the use of a nitrogen phosphorous detector (NPD). This method is simple, reproducible, selective, and applicable in both clinical and pharmacokinetic studies.
Kintz
P
P
Institut de Médecine Légale, Strasbourg, France.
Tracqui
A
A
Mangin
P
P
Lugnier
A
A
Chaumont
A
A
D002849:Chromatography, Gas; D006801:Humans; D007202:Indicators and Reagents; D013034:Sparteine / Q000032:analysis* / Q000097:blood / Q000652:urine
D007202:Indicators and Reagents; D013034:Sparteine