title: Solid phase extraction and HPLC determination of spiramycin in plasma and vitreous concentrations.
abstract: A method for extracting spiramycin by an octadecylsilica cartridge is described for plasma or vitreous samples. The macrolide antibiotic is then measured by reversed-phase HPLC with UV detection. The limit of detection is estimated to be 50 ng/mL. The coefficient of variation for the procedure is 6.1% and 5.2% for the range of concentrations 0.2 micrograms/mL and 10 micrograms/mL respectively. By this method, pharmacokinetic profiles were performed for five adult patients. Spiramycin could be accurately measured in the vitreous humour, allowing the determination of antibiotic at its site of action.
authors.affiliation: Laboratoire de Pharmacologie, Centre Régional de Pharmacovigilance, CHU Morvan, Brest, France.
authors.forename: D
authors.initials: D
authors.lastname: Carlhant
authors.forename: M A
authors.initials: MA
authors.lastname: Le Bot
authors.forename: Y
authors.initials: Y
authors.lastname: Guedes
authors.forename: C
authors.initials: C
authors.lastname: Riche
authors.forename: F
authors.initials: F
authors.lastname: Mimouri
authors.forename: J
authors.initials: J
authors.lastname: Colin
authors.forename: F
authors.initials: F
authors.lastname: Berthou
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003201:Computers; D006801:Humans; D007933:Leucomycins / Q000032:analysis / Q000097:blood* / Q000493:pharmacokinetics; D009883:Ophthalmic Solutions; D014822:Vitreous Body / Q000032:analysis*
chemical_list: D007933:Leucomycins; D009883:Ophthalmic Solutions