New high O2 carrying perfluorochemical emulsions: toxicity, radiosensitivity of GM-CFC and development of metastases in mice.
The effects of two new concentrated perfluorochemical emulsions based on F-66E and PFOB, which carry significantly more oxygen than Fluosol-DA 20%, were tested on normal tissues (toxicity and radiation response) and on the development of metastases from Lewis Lung Carcinoma (3LL) in female C57 BL/6 mice. Twenty one days after injection of F-66E or PFOB emulsions (15 ml/kg body weight), the spleen and liver weights were significantly increased but had returned to normal after 2-3 months. Splenomegaly already observed in 3LL bearing mice was significantly increased by F-66E emulsion injection. The radiosensitivity of GM-CFC was not altered when unanesthetized GM-CFC was not altered when unanesthetized mice were pretreated with F-66E emulsions and/or carbogen 1 hr prior to and during irradiation. The rate of tumor take and the period before detection of tumors were not modified when an emulsion of F-66E was injected simultaneously or 10 days after 3LL cells. Mean survival of mice, and the number of metastases on lung surfaces were similar in F-66E injected mice and control mice.
Lartigau
E
E
Laboratoire de Radiobiologie Cellulaire (Unité Inserm 247), Institut Gustave-Roussy, Villejuif, France.
Thomas
C
C
Le Blanc
M
M
Riess
J
J
Long
D
D
Long
C
C
Malaise
E P
EP
Guichard
M
M
D000818:Animals; D005260:Female; D005466:Fluorocarbons / Q000633:toxicity*; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000528:radiation effects*; D006842:Hydrocarbons, Brominated; D008175:Lung Neoplasms / Q000556:secondary*; D051379:Mice; D008810:Mice, Inbred C57BL; D009929:Organ Size / Q000187:drug effects; D011836:Radiation Tolerance / Q000187:drug effects
D005466:Fluorocarbons; D006842:Hydrocarbons, Brominated; C046505:bis(perfluoro-n-hexyl)-1,2-ethylene; C003072:perflubron