title: Effect of catalytic hydrogenation of Tetrahymena ciliary phospholipid fatty acids on ciliary phospholipase A activity.
abstract: Detached Tetrahymena cilia were treated for increasing periods of time with the homogeneous hydrogenation catalyst palladium di(sodium alizarine monosulphonate). This caused a 4-70% reduction in the number of double bonds in phospholipid-bound fatty acids and a concurrent decrease in membrane fluidity as detected by ESR measurements. Ciliary phospholipase A activity was markedly inhibited when as little as 13% of the fatty acid double bonds had been hydrogenated, suggesting that the enzyme activity is very sensitive to changes in membrane fluidity.
authors.affiliation: Institute of Biochemistry, Hungarian Academy of Sciences, Szeged.
authors.forename: I
authors.initials: I
authors.lastname: Horváth
authors.forename: L
authors.initials: L
authors.lastname: Vigh
authors.forename: T
authors.initials: T
authors.lastname: Pali
authors.forename: G A
authors.initials: GA
authors.lastname: Thompson
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D006865:Hydrogenation; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000502:physiology; D009942:Organometallic Compounds / Q000494:pharmacology; D010165:Palladium / Q000494:pharmacology; D010740:Phospholipases / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D013768:Tetrahymena / Q000187:drug effects / Q000201:enzymology*
chemical_list: D005231:Fatty Acids, Unsaturated; D008563:Membrane Lipids; D009942:Organometallic Compounds; D010743:Phospholipids; D010165:Palladium; C044770:palladium sulfonated alizarine; D010740:Phospholipases