Lipid peroxidation. Definition of experimental conditions for selective study of the propagation and termination phases.
To find experimental conditions to selectively study the propagation phase of lipoperoxidation we studied the lipoperoxidation, catalyzed by FeCl2, of liposomes in a buffering condition where Fe2+ autoxidation and oxygen active species generation does not occur. Liposomes from egg yolk phosphatidylcholine, prepared by vortex mixing, do not oxidize Fe2+; on the contrary they oxidize Fe2+ when prepared by ultrasonic irradiation. Dimyristoyl phosphatidylcholine liposomes prepared by ultrasonic irradiation do not oxidize Fe2+. During sonication polyunsaturated fatty acid residues autoxidize and lipid hydroperoxides (LOOH) are generated. Only when LOOH are present in the liposomes Fe2+ oxidizes and its rate of oxidation depends on the amount of LOOH in the assay. The reaction results in the generation of both LOOH and thiobarbituric acid reactive material (TBAR); it is inhibited by butylated hydroxytoluene and has a acidic pH optimum; it is not inhibited by catalase and OH scavengers. The reaction studied, thus, appears to be the chain branching and propagation phase of lipoperoxidation. When we studied the dependence of Fe2+ oxidation, LOOH and TBAR generation on FeCl2 concentration, we observed that at high FeCl2 concentrations the termination phase of lipoperoxidation was prevalent. Thus, by selecting the appropriate FeCl2 concentration the proposed experimental system allows study of either the propagation or the termination phase of lipoperoxidation.
Tadolini
B
B
Dipartimento di Biochimica, Università di Bologna, Italy.
Fiorentini
D
D
Landi
L
L
Cabrini
L
L
D005296:Ferrous Compounds; D005609:Free Radicals; D007700:Kinetics; D015227:Lipid Peroxidation; D008081:Liposomes; D010084:Oxidation-Reduction; D010713:Phosphatidylcholines
D005296:Ferrous Compounds; D005609:Free Radicals; D008081:Liposomes; D010713:Phosphatidylcholines; C029451:ferrous chloride