Enhanced chemiluminescence in the peroxidase-luminol-H2O2 system: anomalous reactivity of enhancer phenols with enzyme intermediates.
Phenols which markedly enhance chemiluminescence in the horseradish peroxidase catalysed oxidation of luminol by hydrogen peroxide show anomalously high reactivity (by factors of approximately 10(2) compared with published Hammett correlations) in the reduction of the enzyme intermediates, Compound I and Compound II. The results support the hypothesis that efficient production of phenoxy radicals from phenols is a necessary criterion for chemiluminescence enhancer action.
Hodgson
M
M
Radiation and Biophysical Chemistry Laboratory, School of Chemistry, University of Newcastle upon Tyne, UK.
Jones
P
P
D006735:Horseradish Peroxidase / Q000378:metabolism*; D006861:Hydrogen Peroxide; D007700:Kinetics; D008163:Luminescent Measurements; D008165:Luminol; D010544:Peroxidases / Q000378:metabolism*; D010636:Phenols; D011724:Pyridazines
D010636:Phenols; D011724:Pyridazines; D008165:Luminol; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D010544:Peroxidases