[The effect of diltiazem (Lachema) on transmural non-homogeneity of histochemical changes due to the Ca-paradox].
Calcium paradox was induced by 2.5 min perfusion of the rat heart (Langedorf's system) at 37 degrees C with calcium-free Krebs--Henseleit solution and subsequent 10 min reperfusion with the same solution containing calcium. Ca2+ depletion itself did not affect the activities of the histochemically studied enzymes (succinate dehydrogenase, lactate dehydrogenase, beta-hydroxybutyric dehydrogenase, phosphorylase, and ATPases). Ca paradox was histochemically characterized by decrease and even disappearance of activities of the given enzymes and by marked transmural nonhomogeneity of these changes exhibiting a decreasing trend towards the endocardium. Diltiazem in the concentration of 0.4 and particularly of 4.0 mumol.l-1 exerted a protective effect manifested by a better preservation of the enzymatic activities.
Tribulová
N
N
Slezák
J
J
Ravingerová
T
T
Okolicány
J
J
Ziegelhöffer
A
A
Holec
V
V
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004110:Diltiazem / Q000494:pharmacology*; D005260:Female; D006321:Heart / Q000187:drug effects*; D008297:Male; D009206:Myocardium / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D011919:Rats, Inbred Strains
D004110:Diltiazem; D002118:Calcium
Transmurálna nehomogénnost' histochemických zmien po Ca-paradoxe a po jeho ovplyvnení diltiazemom (Lachema).