Multiple continuous-flow solid-phase peptide synthesis. Synthesis of an HIV antigenic peptide and its omission analogues.
Multiple continuous-flow solid-phase peptide synthesis was performed on a standard polystyrene-based resin under low-pressure conditions using a simple manually operated synthesizer. Stable-flow resin-packed columns were prepared in small polypropylene flow reactors, adjustable for volume. The concurrent synthesis of 10 peptides was carried out in flow reactors concatenated together; solvents and reactants were passed through this set of columns using moderate overpressure. One decapeptide, H-Val-Tyr-Tyr-Arg-Asp-Ser-Arg-Asn-Pro-Leu-NH2, containing an antigenic determinant of the p31 protein product of the pol gene of the human immunodeficiency virus, and its nine omission analogues were synthesized.
Krchnák
V
V
Léciva-Pharmaceuticals, Czechoslovak Academy of Sciences, Prague.
Vágner
J
J
Mach
O
O
D015488:HIV Antigens; D008722:Methods; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000276:immunology; D011312:Pressure; D012116:Resins, Plant; D014764:Viral Proteins / Q000138:chemical synthesis* / Q000276:immunology
D015488:HIV Antigens; D009842:Oligopeptides; D012116:Resins, Plant; D014764:Viral Proteins