Mirror densitometry for higher sensitivity and resolution.
In order to increase the pathway of the light inside a gel (or autoradiogram) during scanning, it is placed on top of a mirror and the densitometry is performed in a vertical reflection mode. As the light passes the gel a second time after being reflected by the mirror, the absorbance is nearly doubled. This increase in absorbance results in higher sensitivity and resolution.
Taube
D
D
Max-Planck-Institut für experimentelle Medizin, Forschungstelle Neurochemie, Göttingen, Federal Republic of Germany.
Neuhoff
V
V
D001345:Autoradiography; D003720:Densitometry / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D005069:Evaluation Studies as Topic; D008029:Lighting; D055095:Optics and Photonics; D011506:Proteins / Q000302:isolation & purification
D011506:Proteins