The subunit composition of factor XIII proteins in normal and factor XIII deficient plasma and serum analysed by line immunoelectrophoresis.
Line immunoelectrophoresis was used for the characterization of human coagulation factor XIII in normal and factor XIII-deficient plasma and serum. In normal plasma, two immunoreactive factor XIII proteins termed XIII-p (Mr 330,000 Da) and XIII-s (Mr 170,000 Da) were identified within the beta-globulin fraction. Their reactions with monospecific antisera against known factor XIII subunits, suggests that the factor XIII-p and -s proteins recognized represent the factor XIII a2b2 tetramer and -b2 dimer, respectively. The concentration of the XIII-p protein was, in normal plasma, approximately 6 mg/l, in plasma from a case of congenital factor XIII, approximately 1 mg/l and in normal serum approximately 3 mg/l. The concentration of the XIII-s protein (approximately 18 mg/l) was unaffected by the coagulation process and normal in plasma and serum from the case of congenital factor XIII deficiency. Thus, the present investigation indicates the presence of an excess of the anticatalytic, DNA-binding factor XIII carrier protein in normal as well as in factor XIII-deficient plasma and serum.
Krøll
J
J
Department of Clinical Chemistry, Rigshospitalet, University of Copenhagen, Denmark.
D005176:Factor XIII / Q000032:analysis*; D005177:Factor XIII Deficiency / Q000097:blood*; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods; D010949:Plasma / Q000032:analysis
D005176:Factor XIII