Effects of bile acids on liver cell injury by cultured supernatant of activated liver adherents cells.
When heat-killed Propionibacterium acnes (P. acnes) is intravenously injected into mice followed by an intravenous injection of a small amount of lipopolysaccharide (LPS) 7 days later, most of the mice die of massive hepatic cell necrosis within 24 hours of LPS injection. In addition, when the liver adherent cells including Kupffer cells are separated from the mice 7 days after P. acnes injection and incubated in vitro with LPS, remarkable activity of the cytotoxic factor is found in the culture supernatant. This cytotoxic factor is thought to cause liver injury. Using this experimental model, the effects of various bile acids on liver cell injury were studied. As a result, ursodeoxycholic acid and dehydrocholic acid suppressed liver cell injury induced by the cytotoxic factor. However, cholic acid, deoxycholic acid and chenodeoxycholic acid did not have any hepatocytoprotective effects.
Mizoguchi
Y
Y
Third Department of Internal Medicine, Osaka City University Medical School, Japan.
Kodama
C
C
Sakagami
Y
Y
Seki
S
S
Kobayashi
K
K
Yamamoto
S
S
Morisawa
S
S
D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D056486:Chemical and Drug Induced Liver Injury; D002793:Cholic Acids / Q000494:pharmacology*; D003603:Cytotoxins / Q000096:biosynthesis*; D003840:Deoxycholic Acid / Q000031:analogs & derivatives*; D008099:Liver / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D014580:Ursodeoxycholic Acid / Q000494:pharmacology*
D002793:Cholic Acids; D003603:Cytotoxins; D003840:Deoxycholic Acid; D014580:Ursodeoxycholic Acid