title: Enzymatic degradation and quantitative lectin labeling for characterizing glycoconjugates which act as lectin acceptors in cat submandibular gland.
abstract: Sites of binding of eight different lectins (LTA, UEA I, WGA, SBA, DBA, CON A, PNA, RCA I) to cat submandibular gland were studied after exposure of tissue sections to sialidase, alpha-fucosidase, beta-galactosidase, alpha-mannosidase, beta-N-acetylglucosaminidase. All lectins were affected by enzymatic predigestion and the labeling of individual lectins was highly dependent upon the glycosidase used to pretreat the sections. Glycoconjugates of demilunar, acinar and ductal cells exhibited a different composition of terminal sequences. For example, fucose proved to form the disaccharide fucose-galactose in demilunar and acinar cells, whereas it was present with the sequence fucose-N-acetyl-D-glucosamine in striated duct cells. Sialic acid participated both to the terminal sequence sialic acid-galactose and sialic acid-N-acetyl-D-galactosamine either in demilunar or in ductal cells. Lectin labeling combined with glycosidase digestion was also helpful in verifying the influence of neighbouring oligosaccharides on the affinity of lectins for the respective sugars.
authors.affiliation: Dipartimento di Biologia Cellulare, Universitá di Camerino, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Menghi
authors.forename: D
authors.initials: D
authors.lastname: Accili
authors.forename: A M
authors.initials: AM
authors.lastname: Bondi
authors.forename: M G
authors.initials: MG
authors.lastname: Gabrielli
mesh_terms: D000818:Animals; D002415:Cats; D006001:Glycoconjugates / Q000032:analysis*; D006026:Glycoside Hydrolases / Q000378:metabolism; D008297:Male; D011975:Receptors, Mitogen / Q000032:analysis*; D013363:Submandibular Gland / Q000032:analysis*
chemical_list: D006001:Glycoconjugates; D011975:Receptors, Mitogen; D006026:Glycoside Hydrolases