Determination of doxycycline in bovine tissues and body fluids by high-performance liquid chromatography using photodiode array ultraviolet-visible detection.
A sensitive and selective high-performance liquid chromatographic assay was developed to quantitate doxycycline concentrations in bovine tissues and body fluids. The method involved sonication of doxycycline-spiked minced tissues with appropriate solutions to extract tissue-bound drug. Acetonitrile:85% phosphoric acid:water (20:2:78) was added to doxycycline-spiked serum or urine. The mixtures were ultrafiltered through 30,000 or 10,000 Da molecular weight cutoff microseparation systems. Separation was obtained by a reversed-phase microbore column. Absorbance of the column effluent was measured by an ultraviolet-visible photodiode array detector scanning from 235 to 380 nm and/or a photometric detector operated at 268 or 345 nm. Chromatographic peak homogeneity was evaluated by three-dimensional spectrochromatograms, contour maps, and absorbance ratios.
Riond
J L
JL
Laboratory of Toxicokinetics, College of Veterinary Medicine, North Carolina State University, Raleigh 27606.
Hedeen
K M
KM
Tyczkowska
K
K
Riviere
J E
JE
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004318:Doxycycline / Q000032:analysis* / Q000097:blood / Q000652:urine; D007202:Indicators and Reagents; D007679:Kidney Medulla / Q000032:analysis; D008099:Liver / Q000032:analysis; D009132:Muscles / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet
D007202:Indicators and Reagents; D004318:Doxycycline