Measurement of estrone-3-glucuronide in urine by rapid, homogeneous time-resolved fluoroimmunoassay.
We describe a liquid-phase nonseparation time-resolved fluorescence immunoassay for measuring estrone-3-glucuronide in undiluted urine. The sensitivity, specificity, and accuracy are similar to those for a conventional separation fluoroimmunoassay or radioimmunoassay, but the speed, convenience, precision, reliability, and clinical utility of the new method are more advantageous. The labeled antigen, a fluorescent europium chelate covalently linked to estrone-3-glucuronide, is incubated for 10 min with a limited concentration of polyclonal or monoclonal antibodies to estrone-3-glucuronyl-6-bovine serum albumin and 10 microL of standard or sample (undiluted urine) in microtiter wells. The fluorescence emanating from the antibody-free label, which is proportional to the concentration of estrone-3-glucuronide in the standard or sample, is then measured in a time-resolved fluorometer. The method is useful for monitoring ovarian function in women.
Barnard
G
G
Department of Hormone Research, Weizmann Institute of Science, Rehovot, Israel.
Kohen
F
F
Mikola
H
H
Lövgren
T
T
D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D004970:Estrone / Q000031:analogs & derivatives* / Q000652:urine; D005063:Europium; D005260:Female; D005456:Fluorescent Dyes; D005470:Fluorometry; D006801:Humans; D007118:Immunoassay; D011786:Quality Control; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D005456:Fluorescent Dyes; C015856:estrone-3-glucuronide; D004970:Estrone; D005063:Europium