Postlabeling analysis of indigenous aromatic DNA adducts in mouse myocardium during aging.
The possible presence of aromatic chemicals covalently linked to DNA (aromatic adducts) was investigated in heart cells during aging of the C57BL/6Nia mouse. Heart DNAs were isolated from untreated mice of different ages and analyzed by 32P-postlabeling assays. To determine low levels of adducts, assays were carried out in which aromatic adducts were first isolated by phase transfer to 1-butanol, then labeled with excess, carrier-free [gamma-32P]ATP. This analysis showed that the number and frequency of aromatic adducts varied between DNA samples. Several adducts were present in all mouse DNA preparations and were more abundant in 32P-maps of senescent heart DNA. The results suggest that genomes of myocytes have a higher steady-state level of DNA damage in old animals which could adversely affect cell function.
Gaubatz
J W
JW
Department of Biochemistry, University of South Alabama, College of Medicine, Mobile 36688.
D000375:Aging / Q000378:metabolism*; D000818:Animals; D004247:DNA / Q000032:analysis*; D004249:DNA Damage; D004260:DNA Repair; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009206:Myocardium / Q000032:analysis*; D010761:Phosphorus Radioisotopes
D010761:Phosphorus Radioisotopes; D004247:DNA