Methods for evaluating the acrosomal status of mammalian sperm.
A full understanding of the acrosome reaction is central to understanding sperm function. Acrosomal status can be determined on living, motile sperm in only a few mammalian species. For other species, many light microscopic methods have been developed, including colored stains for bright-field microscopy, and probes for fluorescence microscopy. We review the existing methods and the criteria that should be considered in the choice of an assay.
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Department of Obstetrics and Gynecology, School of Medicine, University of California, Davis 95616.
Meizel
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D000177:Acrosome / Q000648:ultrastructure*; D000818:Animals; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D008297:Male; D008322:Mammals; D008853:Microscopy / Q000379:methods; D013094:Spermatozoa / Q000648:ultrastructure*
D005456:Fluorescent Dyes