Non-selective afferent innervation develops in embryonic mouse spinal cord-dorsal root ganglia explants chronically exposed to GM1 ganglioside.
Projection patterns of dorsal root ganglion afferents within organotypic explants of fetal mouse spinal cord were examined histologically using a horseradish peroxidase staining method. All explants were also examined electrophysiologically and showed spontaneous and evoked bioelectric discharges, which were evenly distributed across the cord explants. Explants chronically grown in a serum-free medium containing the monosialoganglioside GM1 failed to develop dorsal cord selectivity as was previously observed when a mixture of gangliosides was used. It therefore appears that not all gangliosides can induce selectivity in this model.
Baker
R E
RE
Netherlands Institute for Brain Research, Amsterdam.
Janzen
D G
DG
D000818:Animals; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D005677:G(M1) Ganglioside / Q000494:pharmacology*; D005727:Ganglia, Spinal / Q000187:drug effects / Q000196:embryology* / Q000502:physiology; D006735:Horseradish Peroxidase; D066298:In Vitro Techniques; D051379:Mice; D013116:Spinal Cord / Q000187:drug effects / Q000196:embryology* / Q000502:physiology; D013997:Time Factors
D005677:G(M1) Ganglioside; D006735:Horseradish Peroxidase