Solid phase extraction and HPLC determination of spiramycin in plasma and vitreous concentrations.
A method for extracting spiramycin by an octadecylsilica cartridge is described for plasma or vitreous samples. The macrolide antibiotic is then measured by reversed-phase HPLC with UV detection. The limit of detection is estimated to be 50 ng/mL. The coefficient of variation for the procedure is 6.1% and 5.2% for the range of concentrations 0.2 micrograms/mL and 10 micrograms/mL respectively. By this method, pharmacokinetic profiles were performed for five adult patients. Spiramycin could be accurately measured in the vitreous humour, allowing the determination of antibiotic at its site of action.
Carlhant
D
D
Laboratoire de Pharmacologie, Centre Régional de Pharmacovigilance, CHU Morvan, Brest, France.
Le Bot
M A
MA
Guedes
Y
Y
Riche
C
C
Mimouri
F
F
Colin
J
J
Berthou
F
F
D002851:Chromatography, High Pressure Liquid; D003201:Computers; D006801:Humans; D007933:Leucomycins / Q000032:analysis / Q000097:blood* / Q000493:pharmacokinetics; D009883:Ophthalmic Solutions; D014822:Vitreous Body / Q000032:analysis*
D007933:Leucomycins; D009883:Ophthalmic Solutions