Phosphorylation of peripherin, an intermediate filament protein, in mouse neuroblastoma NIE 115 cell line and in sympathetic neurons.
Peripherin, an intermediate filament protein, described recently, is expressed in well defined neuronal populations. We studied the phosphorylation, in vivo, of this protein in mouse neuroblastoma NIE 115 cell line and in sympathetic neurons labelled with [32P]-orthophosphate. The autoradiograms of proteins separated on two-dimensional polyacrylamide gels were compared with the Coomassie-blue stainings. The results show that peripherin occurs as a mixture of phosphorylated and non-phosphorylated isoforms, and that these forms coexist in both differentiated and non-differentiated cells. We demonstrate by cleavage at the unique tryptophan residue, a characteristic shared by most other intermediate filament proteins (IFP), that the phosphorylation sites are located on the amino-terminal half of peripherin as it is for vimentin and desmin. These results are discussed in relation to the organization of the filamentous network constituted by peripherin.
Huc
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College de France, Laboratoire de Biochimie Cellulaire, Paris.
Escurat
M
M
Djabali
K
K
Derer
M
M
Landon
F
F
Gros
F
F
Portier
M M
MM
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D005728:Ganglia, Sympathetic / Q000378:metabolism*; D006801:Humans; D007381:Intermediate Filament Proteins / Q000302:isolation & purification / Q000378:metabolism*; D008562:Membrane Glycoproteins; D051379:Mice; D009419:Nerve Tissue Proteins; D009447:Neuroblastoma / Q000032:analysis / Q000378:metabolism* / Q000473:pathology; D009474:Neurons / Q000378:metabolism*; D009479:Neuropeptides / Q000302:isolation & purification / Q000378:metabolism*; D064531:Peripherins; D010766:Phosphorylation; D014746:Vimentin / Q000302:isolation & purification / Q000378:metabolism
D007381:Intermediate Filament Proteins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; C578994:PRPH protein, human; D064531:Peripherins; D014746:Vimentin