Cytochromes P-450 in the intestinal mucosa of man.
With monoclonal antibodies against cytochrome P-450(5) and P-450(4,5,6), 52 and 54 kDa bands are visualized in microsomes from proximal as well as distal human small intestine. These bands most probably correspond to cytochrome P-450(5) and P-450(4), respectively. This and several other cytochrome P-450 related proteins are present in hepatic microsomes from the same patient. In both hepatic and intestinal microsomes from this patient cytochrome P-450(8) is hardly detectable. In contrast to the small intestine and liver, large intestinal tissue from several other patients does not contain cytochrome P-450(5). Here the 54 kDa isoenzyme, possibly cytochrome P-450(4), is most prominent. Earlier we found a higher content of total cytochrome P-450 in the proximal as compared to the distal small intestine. A similar distribution is now found with regard to aldrin epoxidase activity.
Peters
W H
WH
Department of Medicine, St. Radboud University Hospital, Nijmegen, The Netherlands.
Kremers
P G
PG
D003577:Cytochrome P-450 Enzyme System / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007413:Intestinal Mucosa / Q000201:enzymology*; D007527:Isoenzymes / Q000032:analysis*; D008099:Liver / Q000201:enzymology; D008970:Molecular Weight
D007527:Isoenzymes; D003577:Cytochrome P-450 Enzyme System