Structure of the phosphosaccharide-inositol core of the Leishmania donovani lipophosphoglycan.
The phosphosaccharide-inositol core of the lipophosphoglycan of Leishmania donovani was generated by treatment of the glycoconjugate with mild acid and digestion with phosphatidylinositol-specific phospholipase C. The core was purified and examined by one- and two-dimensional 1H-1H NMR and by methylation analysis. From the results, the carbohydrate core was elucidated as a phosphosaccharide attached to the inositol residue of the lyso-alkylphosphatidylinositol anchor of lipophosphoglycan as follows: PO4----6GalP(alpha 1----6)GalP(alpha 1----3)Galf(alpha 1----3)ManP(alpha 1----3)ManP(alpha 1----4)GlcNP(alpha 1----6)myo-inositol. The presence of an internal galactofuranose residue is highly unusual and the ManP(alpha 1----4)GlcNP(alpha 1----6)myo-inositol sequence is homologous to the respective portion of the glycosylphosphatidylinositol anchors reported for both the Trypanosoma brucei variant surface glycoprotein and the rat brain Thy-1 glycoprotein.
Turco
S J
SJ
Department of Biochemistry, University of Kentucky Medical Center, Lexington 40356.
Orlandi
P A
PA
Homans
S W
SW
Ferguson
M A
MA
Dwek
R A
RA
Rademacher
T W
TW
D000818:Animals; D002240:Carbohydrate Sequence; D002849:Chromatography, Gas; D006028:Glycosphingolipids / Q000032:analysis*; D007893:Leishmania donovani / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000032:analysis*; D013403:Sugar Phosphates / Q000032:analysis*
D006028:Glycosphingolipids; D011134:Polysaccharides; D013403:Sugar Phosphates; C008290:lipophosphonoglycan