Separation of basic drugs by high-performance liquid chromatography on a silica column using a methanol-ethylenediamine buffer.
The application of methanol-aqueous ethylenediamine-ammonium nitrate eluents has been investigated for the high-performance liquid chromatographic separation of basic drugs on silica stationary phases. These eluents were shown to be more reproducible than previously studied systems based on methanol-aqueous ammonia-ammonium nitrate eluents. The effects of different eluent pH and buffer concentrations have been examined.
Smith
R M
RM
Department of Chemistry, Loughborough University of Technology, Leics, U.K.
Rabuor
J O
JO
D002851:Chromatography, High Pressure Liquid; D005029:Ethylenediamines; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D000432:Methanol; D004364:Pharmaceutical Preparations / Q000032:analysis*
D005029:Ethylenediamines; D007202:Indicators and Reagents; D004364:Pharmaceutical Preparations; D000432:Methanol