Liquid chromatographic determination of ivermectin in bovine serum.
A rapid, sensitive method is described for the determination of ivermectin concentrations in bovine serum. Ivermectin is extracted by passing a sample through a reverse-phase C18 cartridge. A silica-packed cartridge is used to purify the extract further. Ivermectin is quantitated by liquid chromatography with detection at 245 nm. Recoveries were 95 +/- 4% for samples fortified with 20 ppb ivermectin. Concentrations as low as 2 ppb can be detected in 5 g samples.
Oehler
D D
DD
U.S. Department of Agriculture, U.S. Livestock Insects Laboratory, Kerrville, TX 78029-0232.
Miller
J A
JA
D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid; D007202:Indicators and Reagents; D007559:Ivermectin / Q000097:blood*; D012997:Solvents
D007202:Indicators and Reagents; D012997:Solvents; D007559:Ivermectin