title: D-glucose uptake is increased in jejunal brush-border membrane vesicles from hyperthyroid chicks.
abstract: Jejunal brush-border membrane vesicles were harvested from 4-week old chicks whose thyroid status had been altered either by a daily injection of 20 micrograms T3 for 1 week or which through the preceding 4 weeks had received propylthiouracil and that had been repleted with either 20 or 80 micrograms T3 in divided doses within 48 h. T3 markedly stimulated D-glucose uptake in brush-border membrane vesicles in the presence of an outside/inside (100/0 mmol/l) Na+ gradient. T3 administration had no detectable influence on the Na+ permeability of the isolated vesicles. The effect of the thyroid hormone on Na+ gradient-driven D-glucose uptake was fully preserved at zero transmembrane potential difference. These findings exclude that T3 stimulates Na+-dependent D-glucose transport in the small intestine through changes of the electrochemical Na+ gradient or through alteration of the transmembrane potential difference. Tracer exchange experiments under equilibrium and voltage-clamp conditions revealed a significantly shorter half-time of D-glucose uptake in brush-border membrane vesicles from T3-treated chicks. Kinetic analysis showed that T3 administration significantly increases the apparent maximal velocity of D-glucose transport in brush-border membrane vesicles, whereas the apparent Km values were virtually unaltered. From these data we conclude that T3 increases the activity of Na+-dependent D-glucose carriers in the brush-border membrane. This is interpreted as consistent with a greater rate of D-glucose absorption from the intestinal lumen under conditions of hyperthyroidism.
authors.affiliation: Department of Biochemistry and Experimental Medicine, Child's Health Center, Warsaw, Poland.
authors.forename: H
authors.initials: H
authors.lastname: Debiec
authors.forename: H S
authors.initials: HS
authors.lastname: Cross
authors.forename: M
authors.initials: M
authors.lastname: Peterlik
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002645:Chickens; D005947:Glucose / Q000378:metabolism*; D006980:Hyperthyroidism / Q000378:metabolism*; D007408:Intestinal Absorption / Q000187:drug effects; D007583:Jejunum / Q000378:metabolism*; D008297:Male; D008871:Microvilli / Q000378:metabolism; D014284:Triiodothyronine / Q000008:administration & dosage
chemical_list: D014284:Triiodothyronine; D005947:Glucose