Production and isolation of large quantities of monoclonal antibody using serum-free medium and fast protein liquid chromatography.
A method for the production and purification of monoclonal antibody on a large laboratory scale is described. It involves the growth of monoclonal antibodies in serum-free medium, ultrafiltration through an Amicon XM100A filter and fast protein liquid chromatography on a mono Q column with an ionic strength and pH elution gradient. Up to 30 mg of antibody per litre of cell culture supernatant was purified in one day. The procedure can be carried out at 4 degrees.
Stocks
S J
SJ
Department of Chemistry, University of British Columbia, Vancouver, Canada.
Brooks
D E
DE
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000379:methods; D003470:Culture Media; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D051381:Rats
D000911:Antibodies, Monoclonal; D003470:Culture Media