title: Competitive binding of gangliosides and glycophorin to wheat germ agglutinin.
abstract: Gangliosides and glycophorin are receptors for wheat germ agglutinin. The competitive binding of these molecules to wheat germ agglutinin is studied by electron spin resonance spectroscopy with spin labels attached to the oligosaccharide chains of gangliosides. Evidence shows that glycophorin is more accessible to wheat germ agglutinin binding than gangliosides. The interactions of gangliosides and glycophorin in liposomes is disrupted on low level binding of WGA.
authors.affiliation: Department of Chemistry, Indiana University, Selangor, Malaysia.
authors.forename: P M
authors.initials: PM
authors.lastname: Lee
authors.forename: K H
authors.initials: KH
authors.lastname: Lee
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D002417:Cattle; D005732:Gangliosides / Q000378:metabolism*; D006021:Glycophorins / Q000378:metabolism*; D008053:Lipid Mobilization; D008081:Liposomes; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic / Q000032:analysis*; D011975:Receptors, Mitogen / Q000032:analysis*; D012795:Sialoglycoproteins / Q000378:metabolism*; D012996:Solutions; D013113:Spin Labels; D013329:Structure-Activity Relationship; D014909:Wheat Germ Agglutinins / Q000378:metabolism*
chemical_list: D005732:Gangliosides; D006021:Glycophorins; D008081:Liposomes; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; D011975:Receptors, Mitogen; D012795:Sialoglycoproteins; D012996:Solutions; D013113:Spin Labels; D014909:Wheat Germ Agglutinins; C034993:ganglioside receptor; C027503:wheat germ agglutinin receptor