[An analysis of factors affecting efficiency in obtaining transgenic mice].
Microinjection of exogenous DNA into male pronucleus at early stages of its formation leads to greater yield of transgenic mice than DNA introduction into male pronucleus at later stages. Dulbecco medium is more suitable for manipulation with murine zygotes than HEPES-containing Whitten medium. On the other side, albumin-free Whitten medium is better for dissolving exogenous DNA for introduction into murine zygote pronucleus as compared to Dulbecco medium and TE buffer.
Isabekov
B S
BS
Koniukhov
B V
BV
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects; D003470:Culture Media; D004247:DNA / Q000008:administration & dosage; D005069:Evaluation Studies as Topic; D005260:Female; D005818:Genetic Engineering / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic / Q000235:genetics*; D012996:Solutions; D013077:Sperm Head / Q000187:drug effects; D013084:Sperm-Ovum Interactions / Q000187:drug effects; D013997:Time Factors; D015053:Zygote / Q000187:drug effects
D003470:Culture Media; D012996:Solutions; D004247:DNA
Analiz faktorov, vliiaiushchikh na éffektivnost' polucheniia transgennykh mysheĭ.