Clindamycin and tetracycline as immunomodulating agents: an in vivo study.
The present study was undertaken to determine the effects of clindamycin and tetracycline, both intravenously administered, on antibody response to thymus-dependent antigen (PC-KLH) in BALB/c mice. The immunological parameters evaluated were: DPFC/spleen (direct plaque forming-cells), antibody secretion median rate (PC50), heterogeneity index (Hi), number of total splenic lymphocytes and cellular viability. The results showed that clindamycin (i.v.) increased the humoral response; 28 mg/kg was the dose that showed the greatest enhancement (+73%). The PC50 was not affected by clindamycin but Hi decreased at 28 mg/kg and increased at 2.8 mg/kg doses, although neither result was statistically significant. When tetracycline was given i.v., a slight decrease in the anti-PC DPFC number was observed. Although the PC50 was greater at 10 mg/kg (p less than 0.05), Hi was smaller at the 1 mg/kg dose (p less than 0.05).
Corrales
I
I
Department of Microbiology, Faculty of Medicine, Complutense University, Madrid, Spain.
Suarez
A
A
Lima
A
A
Ballestero
S
S
Gómez-Lus
M L
ML
Prieto
J
J
D000276:Adjuvants, Immunologic; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D002981:Clindamycin / Q000494:pharmacology*; D005260:Female; D006433:Hemocyanins / Q000276:immunology; D006462:Hemolytic Plaque Technique; D007166:Immunosuppressive Agents; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010713:Phosphatidylcholines / Q000494:pharmacology; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D013752:Tetracycline / Q000494:pharmacology*
D000276:Adjuvants, Immunologic; D007166:Immunosuppressive Agents; D010713:Phosphatidylcholines; D002981:Clindamycin; D006433:Hemocyanins; D013752:Tetracycline