Isolation, primary structure and bioactivity of schistoflrf-amide, a FMRF-amide-like neuropeptide from the locust, Schistocerca gregaria.
We have isolated a neuropeptide, related to the Phe-Met-Arg-Phe-NH2 family, from the thoracic nervous system of the locust, Schistocerca gregaria, using a purification system based on the radioimmunoassay of high pressure liquid chromatography fractions. The primary sequence of this locust peptide is Pro-Asp-Val-Asp-His-Val-Phe-Leu-Arg-Phe-NH2. The bioactivities of the native and synthetic neuropeptide are identical in both the locust heart and hindleg extensor-tibiae muscle bioassays.
Robb
S
S
AFRC Unit of Insect Neurophysiology and Pharmacology, Dept. of Zoology, University of Cambridge, UK.
Packman
L C
LC
Evans
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PD
D000595:Amino Acid Sequence; D000818:Animals; D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006110:Grasshoppers; D006321:Heart / Q000187:drug effects*; D007301:Insect Hormones / Q000302:isolation & purification* / Q000502:physiology; D008297:Male; D008969:Molecular Sequence Data; D009119:Muscle Contraction / Q000187:drug effects*; D009479:Neuropeptides / Q000302:isolation & purification* / Q000502:physiology
D007301:Insect Hormones; D009479:Neuropeptides; C059573:SchistoFLRFamide