Elastase 1 and chymotrypsin B in pancreatic juice and feces.
A chymotrypsin-like protease was detected along with elastase 1 in pancreatic secretion and stool. This enzyme was isolated from necrobiotic human pancreas, purified, partially characterized and designated as chymotrypsin B. Quantitative studies by rocket immunoelectrophoresis indicated that neither elastase 1 nor chymotrypsin B was degraded during intestinal passage. On the basis of a clinical study, both enzymes were found to reflect pancreatic function.
Sziegoleit
A
A
Department of Gastroenterology, University of Giessen, FRG.
Krause
E
E
Klör
H U
HU
Kanacher
L
L
Linder
D
D
D002918:Chymotrypsin / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000201:enzymology*; D006801:Humans; D007122:Immunoelectrophoresis; D007700:Kinetics; D010196:Pancreatic Elastase / Q000378:metabolism*; D010189:Pancreatic Juice / Q000201:enzymology*
D002918:Chymotrypsin; D010196:Pancreatic Elastase