Reactive blue 2 selectively inhibits P2y-purinoceptor-stimulated surfactant phospholipid secretion from rat isolated alveolar type II cells.
1. The effect of reactive blue 2 on adenosine 5'-triphosphate (ATP), 12-O-tetradecanoylphorbol 13-acetate (TPA) and terbutaline-induced surfactant phospholipid secretion from rat isolated alveolar Type II cells was studied. 2. Reactive blue 2 significantly inhibited ATP-induced surfactant phospholipid secretion, but was without effect on C-kinase agonist (TPA) or beta-adrenoceptor agonist (terbutaline)-stimulated surfactant phospholipid secretion. The IC50 for inhibition of ATP-induced surfactant secretion was 1.5 x 10(-4)M. 3. These data are consistent with a P2y-purinoceptor regulating surfactant phospholipid secretion from isolated Type II cells and support previous work suggesting reactive blue 2 is a specific inhibitor at P2y-purinoceptors.
Rice
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University of Cincinnati, College of Medicine, Department of Pediatrics, Ohio 45267-0541.
Singleton
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FM
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D008297:Male; D010713:Phosphatidylcholines / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D011650:Pulmonary Alveoli / Q000166:cytology / Q000378:metabolism*; D011663:Pulmonary Surfactants / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011983:Receptors, Purinergic / Q000187:drug effects* / Q000378:metabolism; D013726:Terbutaline / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014227:Triazines / Q000494:pharmacology*
D010713:Phosphatidylcholines; D010743:Phospholipids; D011663:Pulmonary Surfactants; D011983:Receptors, Purinergic; D014227:Triazines; C012842:Cibacron Blue F 3GA; D000255:Adenosine Triphosphate; D013726:Terbutaline; D013755:Tetradecanoylphorbol Acetate