Ectopic induction of cartilage and bone by water-soluble proteins from bovine bone using a collagenous delivery vehicle.
A controlled-release delivery vehicle for water-soluble osteogenic proteins from demineralized bone matrix was constructed using purified type I collagen. The water-soluble proteins were isolated from a 4 M GdnHCl extract of bone matrix. Although the water-soluble proteins were capable of inducing cartilage formation in vitro, they were incapable of inducing cartilage or bone in vivo when implanted intramuscularly into mice in the absence of an appropriate delivery vehicle. The collagen-based delivery vehicle alone was also incapable of inducing osteogenesis in vivo. However, when the water-soluble proteins were incorporated into the delivery vehicle, the combination was capable of inducing cartilage and bone 76% of the time. These results demonstrate that it is possible to formulate a controlled-release delivery vehicles for soluble bioactive factors which upon release interact with local responsive cells.
Lucas
P A
PA
Department of Biology, Case Western Reserve University, Cleveland, Ohio 44106.
Syftestad
G T
GT
Goldberg
V M
VM
Caplan
A I
AI
D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use*; D001685:Biological Factors / Q000008:administration & dosage / Q000302:isolation & purification / Q000502:physiology*; D001857:Bone Matrix / Q000032:analysis / Q000502:physiology*; D002356:Cartilage / Q000187:drug effects* / Q000254:growth & development; D002417:Cattle; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology; D051379:Mice; D008808:Mice, Inbred CBA; D010012:Osteogenesis / Q000187:drug effects*; D014677:Pharmaceutical Vehicles / Q000494:pharmacology*; D011506:Proteins / Q000008:administration & dosage / Q000302:isolation & purification / Q000502:physiology*
D001672:Biocompatible Materials; D001685:Biological Factors; D014677:Pharmaceutical Vehicles; D011506:Proteins; D003094:Collagen