Gangliosides of the mouse spinal cord: a comparison in in vivo and in vitro tissues.
Ganglioside profiles in spinal cord from 13-day mouse fetuses, 21-day postnatal and adult mice were compared with those harvested from organotypic cross-sections of fetal mouse spinal cord grown for 28 days in vitro in a serum-free medium. All the major species of gangliosides reported for brain were present both in the in vivo tissue and cultured spinal cord, though not necessarily at each developmental stage examined. Fresh tissues showed increases and decreases in various gangliosides as have been reported for higher brain centers at similar stages of development in mammals and birds. However, qualitative and quantitative differences exist between fresh spinal cord and cultured cord explants as well as between galactose-grown and galactose-free cultures. Spinal cord explants grown in the presence of galactose showed measurable amounts of GM2 and GM3 which were not detected in the control-defined medium-grown cultures. The differences between the two culture groups may be related to interneuronal connectivity patterns.
Baker
R E
RE
Netherlands Institute for Brain Research, Amsterdam.
Guérold
B
B
Dreyfus
H
H
D000375:Aging / Q000378:metabolism*; D000818:Animals; D005690:Galactose / Q000494:pharmacology; D005732:Gangliosides / Q000378:metabolism*; D066298:In Vitro Techniques; D051379:Mice; D008855:Microscopy, Electron, Scanning; D013116:Spinal Cord / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*
D005732:Gangliosides; D005690:Galactose