title: Synthesis of a substrate of protein kinase C and its corresponding phosphopeptide.
abstract: The syntheses of a protein kinase C (PKC) peptide substrate, H-Lys-Arg-Thr-Leu-Arg-OH, and a phosphopeptide analog of the synthetic substrate, H-Lys-Arg-Thr(P)-Leu-Arg-OH, are reported. PKC phosphorylates the peptide with an apparent KM of 0.30 +/- 0.04 mM and an apparent Vmax equal to one-tenth that of histone III-S. The synthesis of the phosphopeptide features a recently developed convenient phosphorylation procedure for serine and threonine using N,N-diethylamino-dibenzylphosphoramidite. A complete characterization of the PKC substrate and its corresponding phosphopeptide by C-H COSY 2D n.m.r. is included.
authors.affiliation: Dept. of Organic Chemistry, Gorlaeus Laboratories, University of Leiden, The Netherlands.
authors.forename: H B
authors.initials: HB
authors.lastname: de Bont
authors.forename: R M
authors.initials: RM
authors.lastname: Liskamp
authors.forename: C A
authors.initials: CA
authors.lastname: O'Brian
authors.forename: C
authors.initials: C
authors.lastname: Erkelens
authors.forename: G H
authors.initials: GH
authors.lastname: Veeneman
authors.forename: J H
authors.initials: JH
authors.lastname: van Boom
mesh_terms: D055598:Chemical Phenomena; D002621:Chemistry; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D010748:Phosphopeptides / Q000138:chemical synthesis; D011493:Protein Kinase C; D013379:Substrate Specificity
chemical_list: D010748:Phosphopeptides; D011493:Protein Kinase C