Lack of effect of selenium on induction of tumors of esophagus and bladder in rats by two nitrosamines.
The effect of differences in level of dietary selenium on the induction of esophageal and bladder tumors in rats by two nitrosamines was investigated. Groups of 20 female F344 rats were given a synthetic diet containing less than 0.05 ppm Se to which selenium (as sodium selenite) was added at the concentration of 0.35, 0.7, 1.4 and 2.1 ppm selenium. These four groups, plus one without added Se, were treated with 20 ml per rat per day, 5 days a week, of a solution of nitrosomethylcyclohexylamine containing 5 mg/liter. A parallel five groups were treated in the same way with a solution of nitrosomethyl-3-carboxypropylamine in drinking water containing 600 mg per liter, as drinking water. Treatment lasted 28 weeks, at which time some animals had developed tumors. A group of 20 rats fed 0, 1.4 and 2.1 ppm Se was not treated with carcinogen. Rats consuming 1.4 ppm or 2.1 ppm Se gained weight more slowly than other groups. There was no significant difference in survival between the five groups treated with each carcinogen but receiving different dietary levels of selenium. Neither was there any significant difference between groups receiving each carcinogen in the incidence of tumors of the esophagus induced by nitrosomethylcyclohexylamine or of tumors of the urinary bladder induced by nitrosomethylcarboxypropylamine. Control rats on the synthetic diets did not survive as well as untreated rats eating regular chow diet. In these conditions there was no effect of dietary selenium levels on the induction of tumors in female rats by the two carcinogenic nitrosamines we used.
Lijinsky
W
W
NCI-Frederick Cancer Research Facility, BRI-Basic Research Program, Frederick, Maryland.
Milner
J A
JA
Kovatch
R M
RM
Thomas
B J
BJ
D000818:Animals; D002273:Carcinogens / Q000008:administration & dosage / Q000494:pharmacology*; D004032:Diet; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004938:Esophageal Neoplasms / Q000139:chemically induced*; D005260:Female; D009602:Nitrosamines / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D011916:Rats, Inbred F344; D012643:Selenium / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D001749:Urinary Bladder Neoplasms / Q000139:chemically induced*
D002273:Carcinogens; D009602:Nitrosamines; C044779:4-(N-nitroso-N-methylamino)butyric acid; D012643:Selenium; C025137:N-nitroso-N-methylcyclohexylamine