Effects of compound 48/80 and exogenous histamine on wound healing in mice.
Compound 48/80 has previously been shown to improve wound healing in rats, presumably through stimulation of histidine decarboxylase activity and mobilization of histamine from mast cells. In the present study, C57Bl/6 mice were wounded by dorsal skin incision followed by treatment with compound 48/80, exogenous histamine, or the combination of 48/80 plus histamine. Skin-breaking strength was significantly increased over saline-injected controls by the combined treatment with 48/80 and histamine. Neither 48/80 or histamine alone had any influence on wound healing. Histamine content of skin at the wound site was significantly reduced by 48/80 treatment, but was unaffected by 48/80 plus histamine or histamine given alone. In contrast, stomach and leg muscle histamine levels were significantly increased beyond those of unwounded, wounded saline- or 48/80-injected mice. These results were also confirmed in CD mice, and are in contrast to findings in rats in which treatment with 48/80 alone significantly improved wound healing of similarly injured animals.
Yoshida
S
S
Department of Nutrition, Rutgers University, New Brunswick, NJ 08903.
Flancbaum
L
L
Fitzpatrick
J C
JC
Berg
R A
RA
Fisher
H
H
D000818:Animals; D005753:Gastric Mucosa / Q000378:metabolism; D006632:Histamine / Q000378:metabolism / Q000494:pharmacology*; D006640:Histidine Decarboxylase / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009132:Muscles / Q000378:metabolism; D012867:Skin / Q000293:injuries / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects*; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology*
D003189:p-Methoxy-N-methylphenethylamine; D006632:Histamine; D006640:Histidine Decarboxylase