Immunohistochemical diagnosis of Neospora caninum in tissue sections.
An avidin-biotin-peroxidase complex immunoperoxidase staining method was developed to detect Neospora caninum in formalin-fixed, paraffin-embedded tissue sections. Specific antiserum to N caninum was made in rabbits and used to probe tissues from dogs naturally and experimentally infected with N caninum. The test detected tachyzoites and bradyzoites of N caninum. A reaction was not observed to Toxoplasma gondii, Hammondia hammondi, Sarcocystis cruzi, S capricanis, S tenella, Besnoitia jellisoni, Caryospora bigenetica, Hepatazoon canis, Atoxoplasma sp, or the organism causing canine dermal coccidiosis. When antiserum made in rabbits to T gondii was used in the test, reaction to N caninum was not observed.
Lindsay
D S
DS
Zoonotic Diseases Laboratory, Beltsville, MD 20705.
Dubey
J P
JP
D000818:Animals; D001717:Birds; D001921:Brain / Q000469:parasitology; D003044:Coccidia / Q000302:isolation & purification*; D003048:Coccidiosis / Q000469:parasitology / Q000662:veterinary*; D004283:Dog Diseases / Q000469:parasitology*; D004285:Dogs; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008099:Liver / Q000469:parasitology; D051379:Mice; D051381:Rats; D012867:Skin / Q000469:parasitology