Characterization of yeasts with high L[+]-lactic acid production: lactic acid specific soft-agar overlay (LASSO) and TAFE-patterns.
Only few yeast strains are known for the high level production of L[+]-lactate. We report indications for the conspecifity of Kluyveromyces thermotolerans (formerly Saccharomyces veronae) strain CBS 4728 with Stamm 42 (formerly Saccharomyces pretoriensis, RADLER 1984). We suggest that Stamm 42 has little, if any relationship to Saccharomyces cerevisiae. Furthermore, we have optimized the method of Subden et al. (1982) for the detection of lactate producing microorganisms. Using this method in a screening with 100 yeast strains of our institute collection, we could not find additional strains with high L[+]-lactate production. This method may provide a useful tool for the molecular cloning of the unique yeast L[+]-LDH1) gene (s).
Witte
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Institut für Mikrobiologie/BioIV, RWTH Aachen, FRG.
Krohn
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Emeis
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D015139:Blotting, Southern; D003429:Cross Reactions; D003470:Culture Media; D004271:DNA, Fungal / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D005285:Fermentation; D007716:Kluyveromyces / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D007773:Lactates / Q000096:biosynthesis*; D009693:Nucleic Acid Hybridization; D012440:Saccharomyces / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D004718:Saccharomycetales / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid
D003470:Culture Media; D004271:DNA, Fungal; D007773:Lactates