[Thrombolytic activity of Bacillus mesentericus at different conditions of nitrogen nutrition of a culture].
The work is concerned with studying the effect exerted by different sources of nitrogen nutrition on the biosynthesis of proteinases with a thrombolytic activity by a variant of Bacillus mesentericus, strain 64, obtained with the aid of analytical selection. Protein substrates taken as a nitrogen source stimulate the synthesis of proteinases by the bacterial culture. These enzymes have a high caseinolytic and thrombolytic activity, and the level of their activity correlates with the amount of a protein substrate added to the medium. Ammonium acetate and succinate are the best stimulants for the formation of proteinases when the salts of mineral and organic acids are used as a source of nitrogen nutrition. In that case, the enzymes have a high thrombolytic activity and a low caseinolytic activity. A semi-synthetic medium with the aforementioned nitrogen-containing compounds as a source of nitrogen nutrition is proposed for the synthesis of thrombolytic proteinase by the variant of B. mesentericus.
Cherkesova
G V
GV
Nesterova
N G
NG
Fetisova
Z S
ZS
D001407:Bacillus / Q000201:enzymology* / Q000254:growth & development; D003018:Clot Retraction; D003470:Culture Media; D010450:Endopeptidases / Q000096:biosynthesis* / Q000378:metabolism; D005342:Fibrinolysis; D006801:Humans; D009584:Nitrogen / Q000378:metabolism*
D003470:Culture Media; D010450:Endopeptidases; D009584:Nitrogen
Tromboliticheskaia aktivnost' u Bacillus mesentericus pri razlichnykh usloviiakh azotnogo pitaniia kul'tury.