[Flow cytometric analysis of T lymphocyte subsets after human bone marrow transplantation using monoclonal antibodies and dual immunofluorescence].
Using monoclonal antibodies and a dual immunofluorescence technique, we studied the abnormalities of T lymphocyte subsets after human marrow transplantation. T lymphocytes bearing HLA-DR antigen increased both in patients with acute and chronic graft-versus-host disease (GVHD). Parallel to a decrease in CD4+ cells, a CD4+ 2H4+ subset decreased and gradually recovered with time while the percentage of CD4+ 4B4+ cells increased. A significant increase of CD8+ cells observed after marrow transplant corresponded mainly to an increase in CD8+ CD11- cells. Patients with chronic GVHD tended to have a higher proportion of CD8+ CD11+ cells. These results indicate abnormalities of T cell subsets among both CD4+ and CD8+ cells after marrow grafting.
Matsue
K
K
Yasue
S
S
Iwabuchi
K
K
Ohtsuka
M
M
Ueda
M
M
Kondo
K
K
Shiobara
S
S
Mori
T
T
Matsuda
T
T
Harada
M
M
D000293:Adolescent; D000328:Adult; D000911:Antibodies, Monoclonal; D016026:Bone Marrow Transplantation / Q000009:adverse effects / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D005455:Fluorescent Antibody Technique; D006086:Graft vs Host Disease / Q000209:etiology / Q000276:immunology; D006801:Humans; D007223:Infant; D007958:Leukocyte Count; D008297:Male; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal