Failure of spermatozoa from T/t mice to fertilize in vitro is overcome by zona drilling.
Failure of epididymal spermatozoa from T/t mutant mice, but not from t/t individuals, to fertilize oocytes in vitro was partially overcome by opening a small aperture in the zona pellucida with acidified Tyrode's solution to permit direct access of the spermatozoon to the vitellus. This study provides a model system to evaluate requirements for successful zona drilling in the treatment of human infertility and further insights into the effects of the t complex on sperm fertility.
Ahmad
T
T
Research Institute and Gynecology, Cleveland Clinic Foundation, Ohio 44195-5240.
Conover
J C
JC
Quigley
M M
MM
Collins
R L
RL
Thomas
A J
AJ
Gwatkin
R B
RB
D000818:Animals; D005260:Female; D005307:Fertilization in Vitro; D006579:Heterozygote; D007248:Infertility, Male / Q000503:physiopathology*; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D010063:Ovum / Q000502:physiology*; D051381:Rats; D013081:Sperm Motility; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000502:physiology*; D015044:Zona Pellucida / Q000502:physiology*