Increased response to growth factors of skin fibroblasts from newborn spontaneously hypertensive rats.
Skin fibroblasts were isolated from newborn spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats (WKY) and cultured with 10% fetal calf serum. The growth rate, determined by the culture dish DNA content, was significantly higher in cells from SHR than from WKY in the presence of 10 and 15% serum. Re-initiation of DNA synthesis by serum and insulin was assessed by 24-h 3H-thymidine incorporation in quiescent cells. Serum (5, 10 and 15%) and insulin (1 microgram/ml) induced a marked stimulation of 3H-thymidine incorporation, and the cell response was significantly higher in SHR than in WKY for both agonists. These results indicate that the hyper-responsiveness to growth factors evident in newborn SHR skin fibroblasts is a genetic defect independent of haemodynamic changes.
Guicheney
P
P
INSERM U 7, Hôpital Necker, Paris, France.
Meyer
P
P
D000818:Animals; D000831:Animals, Newborn; D002455:Cell Division / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006133:Growth Substances / Q000494:pharmacology*; D007106:Immune Sera / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D051381:Rats; D011918:Rats, Inbred SHR / Q000502:physiology*; D011919:Rats, Inbred Strains / Q000502:physiology*; D011921:Rats, Inbred WKY; D012867:Skin / Q000166:cytology / Q000187:drug effects*
D006133:Growth Substances; D007106:Immune Sera; D007328:Insulin; D004247:DNA