Chronic long-term electrostimulation creates a unique metabolic enzyme profile in rabbit fast-twitch muscle.
Long-term low-frequency stimulation (up to 120 days) of rabbit fast-twitch tibialis anterior muscle led, in a first-order-like time course, to changes in enzyme activities of energy metabolism which became stable with ongoing stimulation after 50 days. The glycolytic enzymes decreased to 30-40% of their normal values, but remained 2-3-fold higher than in heart or soleus muscle. The LDH isozyme pattern ultimately resembled that of the slow-twitch soleus muscle. Citrate synthase activity increased 3.7-fold which brought this enzyme to a value 45% above that of heart. These results indicate that chronic stimulation does not simply convert the fast-twitch muscle into a soleus-like slow-twitch muscle, but creates a tissue of unique metabolic properties.
Hood
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DA
Fakultät für Biologie, Universität Konstanz, FRG.
Pette
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D
D000818:Animals; D002950:Citrate (si)-Synthase / Q000378:metabolism; D004558:Electric Stimulation; D004591:Electrophoresis, Polyacrylamide Gel; D004734:Energy Metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D006019:Glycolysis; D007527:Isoenzymes; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000378:metabolism; D008297:Male; D009132:Muscles / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology; D011817:Rabbits
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D002950:Citrate (si)-Synthase