title: Determination of doxycycline in bovine tissues and body fluids by high-performance liquid chromatography using photodiode array ultraviolet-visible detection.
abstract: A sensitive and selective high-performance liquid chromatographic assay was developed to quantitate doxycycline concentrations in bovine tissues and body fluids. The method involved sonication of doxycycline-spiked minced tissues with appropriate solutions to extract tissue-bound drug. Acetonitrile:85% phosphoric acid:water (20:2:78) was added to doxycycline-spiked serum or urine. The mixtures were ultrafiltered through 30,000 or 10,000 Da molecular weight cutoff microseparation systems. Separation was obtained by a reversed-phase microbore column. Absorbance of the column effluent was measured by an ultraviolet-visible photodiode array detector scanning from 235 to 380 nm and/or a photometric detector operated at 268 or 345 nm. Chromatographic peak homogeneity was evaluated by three-dimensional spectrochromatograms, contour maps, and absorbance ratios.
authors.affiliation: Laboratory of Toxicokinetics, College of Veterinary Medicine, North Carolina State University, Raleigh 27606.
authors.forename: J L
authors.initials: JL
authors.lastname: Riond
authors.forename: K M
authors.initials: KM
authors.lastname: Hedeen
authors.forename: K
authors.initials: K
authors.lastname: Tyczkowska
authors.forename: J E
authors.initials: JE
authors.lastname: Riviere
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004318:Doxycycline / Q000032:analysis* / Q000097:blood / Q000652:urine; D007202:Indicators and Reagents; D007679:Kidney Medulla / Q000032:analysis; D008099:Liver / Q000032:analysis; D009132:Muscles / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet
chemical_list: D007202:Indicators and Reagents; D004318:Doxycycline