title: Heat-stable protein that stimulates acid alpha-glucosidase.
abstract: A hot-water extract of bovine spleen and guinea pig liver exhibited the ability to enhance acid alpha-glucosidase activity, with methylumbelliferyl alpha-glucoside, glycogen or maltose as substrate. The level of activator required for maximal stabilization was similar for all three substrates, indicating direct action on the enzyme rather than on substrate. The stimulator was partially purified by chromatography with gel-permeation (apparent Mr 20,000-24,000), ion-exchange and C4 reverse-phase columns. It was retained by a narrow-pore dialysis tubing and destroyed by treatment with Pronase, and is presumably a protein. The stimulating protein protected the enzyme against denaturation by heat or incubation with a buffer of high ionic strength in the absence of substrate. RNA inhibited the enzyme, and the activator protein was able to counteract the effect. Activating material was found in a variety of mouse and rat tissues, as well as human urine.
authors.affiliation: Mental Health Research Institute, University of Michigan, Ann Arbor 48104.
authors.forename: N S
authors.initials: NS
authors.lastname: Radin
authors.forename: A
authors.initials: A
authors.lastname: Shukla
authors.forename: G S
authors.initials: GS
authors.lastname: Shukla
authors.forename: A
authors.initials: A
authors.lastname: Sano
mesh_terms: D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D004789:Enzyme Activation; D006003:Glycogen / Q000378:metabolism; D006168:Guinea Pigs; D006358:Hot Temperature; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D008320:Maltose / Q000378:metabolism; D008970:Molecular Weight; D009928:Organ Specificity; D011506:Proteins / Q000302:isolation & purification / Q000494:pharmacology / Q000502:physiology*; D013154:Spleen / Q000201:enzymology; D013379:Substrate Specificity; D000520:alpha-Glucosidases / Q000378:metabolism*
chemical_list: D011506:Proteins; D008320:Maltose; D006003:Glycogen; D000520:alpha-Glucosidases