[Isolation and study of the properties of specific antisera to [leu]enkephalin].
The immunogenic conjugates of [leu]enkephalin and bovine serum albumin (BSA) with bisdiazobenzidine and 1,4-benzoquinone as bifunctional reagents have been synthesized. The antisera with high titer of antibodies to [leu]enkephalin have been obtained at rabbit [correction of rat] immunization by both conjugates. The antiserum obtained at immunization by conjugate [leu]enkephalin-benzoquinone-BSA possesses the high affinity and sensitivity to [leu]enkephalin.
Rostovtsev
A P
AP
Grigor'iants
O O
OO
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000918:Antibody Specificity / Q000276:immunology*; D001560:Benzidines / Q000494:pharmacology; D016227:Benzoquinones; D001667:Binding, Competitive; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004743:Enkephalin, Leucine / Q000276:immunology*; D006241:Haptens; D007114:Immunization / Q000379:methods; D007124:Immunoenzyme Techniques; D011809:Quinones / Q000276:immunology / Q000494:pharmacology; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000276:immunology
D000906:Antibodies; D001560:Benzidines; D016227:Benzoquinones; D003432:Cross-Linking Reagents; D006241:Haptens; D011809:Quinones; D012710:Serum Albumin, Bovine; C004532:quinone; D004743:Enkephalin, Leucine; C006770:bis(diazo)benzidine
Poluchenie i izuchenie svoĭstv spetsificheskikh antisyvorotok k [leu]énkefalinu.