Effect of catalytic hydrogenation of Tetrahymena ciliary phospholipid fatty acids on ciliary phospholipase A activity.
Detached Tetrahymena cilia were treated for increasing periods of time with the homogeneous hydrogenation catalyst palladium di(sodium alizarine monosulphonate). This caused a 4-70% reduction in the number of double bonds in phospholipid-bound fatty acids and a concurrent decrease in membrane fluidity as detected by ESR measurements. Ciliary phospholipase A activity was markedly inhibited when as little as 13% of the fatty acid double bonds had been hydrogenated, suggesting that the enzyme activity is very sensitive to changes in membrane fluidity.
Horváth
I
I
Institute of Biochemistry, Hungarian Academy of Sciences, Szeged.
Vigh
L
L
Pali
T
T
Thompson
G A
GA
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D006865:Hydrogenation; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000502:physiology; D009942:Organometallic Compounds / Q000494:pharmacology; D010165:Palladium / Q000494:pharmacology; D010740:Phospholipases / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D013768:Tetrahymena / Q000187:drug effects / Q000201:enzymology*
D005231:Fatty Acids, Unsaturated; D008563:Membrane Lipids; D009942:Organometallic Compounds; D010743:Phospholipids; D010165:Palladium; C044770:palladium sulfonated alizarine; D010740:Phospholipases