Determination of trans-phylloquinone in children's serum.
By optimizing the conditions for determining trans-phylloquinone and its metabolite, K-2,3-epoxide, in serum through a two-step HPLC process combined with fluorometric detection after coulometric reduction, we have been able to develop a method applicable to small volumes of serum (200 to 500 microL). The limit of detection (signal-to-noise ratio of 3) was 15 ng/L for trans-phylloquinone, 30 ng/L for K-2,3-epoxide. The trans-phylloquinone concentrations measured by this method in serum from 82 children, ages one to six years, whose results were normal for overall coagulation tests, ranged from 40 to 880 ng/L (median 175 ng/L). We discuss these findings and compare them with vitamin K1(20) values reported for adults.
Moussa
F
F
Hôpital Trousseau, Laboratoire de Biochimie, Paris, France.
Dufour
L
L
Didry
J R
JR
Aymard
P
P
D002648:Child; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003124:Colorimetry; D005260:Female; D006801:Humans; D007202:Indicators and Reagents; D007223:Infant; D008297:Male; D013050:Spectrometry, Fluorescence; D013223:Statistics as Topic; D010837:Vitamin K 1 / Q000031:analogs & derivatives* / Q000097:blood*
D007202:Indicators and Reagents; C016186:vitamin K1 oxide; D010837:Vitamin K 1