[MAC-ELISA for the detection of IgM antibodies to dengue type I virus (rapid diagnosis of dengue type I virus infection)].
The commercial rapid diagnostic reagents for Dengue virus infection is still not available at present. An ABC MAC-ELISA (Avidin Biotin Complex IgM Antibody Capture Enzyme Linked Immunosorbent Assay) for the detection of Dengue type I virus infection has been described. IgG purified from high titer (HI = 2560) human anti-flavivirus serum is labelled with biotin for the rapid diagnosis of Dengue type I virus infection. 389 serum specimens of suspected Dengue Fever patients including 226 HI(+) paired sera and 163 HI(-) paired sera which were collected from 1 to 150 days after onset of the disease in 1988 have been assayed for IgM antibodies to Dengue type I virus. Cut off value is based on the mean of OD490 of 162 Dengue negative serum specimens plus 4 SD. The positivity of IgM antibody increased as the viremia disappeared and IgM antibody for Dengue-1 serum specimens collected 9 days after onset of the disease is 100% detectable.
Chow
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National Institute of Preventive Medicine, Department of Health Taipei, ROC.
Hsu
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D000914:Antibodies, Viral / Q000032:analysis*; D001360:Avidin; D001710:Biotin / Q000378:metabolism; D003715:Dengue / Q000175:diagnosis* / Q000276:immunology; D003716:Dengue Virus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000302:isolation & purification / Q000378:metabolism; D007075:Immunoglobulin M / Q000032:analysis*
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D001360:Avidin; D001710:Biotin