Cloning of cDNA for a novel mouse membrane glycoprotein (gp42): shared identity to histocompatibility antigens, immunoglobulins and neural-cell adhesion molecules.
A full-length clone encoding a murine membrane glycoprotein, gp42, was selected from a mouse fibroblast cDNA expression library by screening with a polyclonal antiserum. The deduced amino acid (aa) sequence indicates that gp42 is a transmembrane protein of 273 aa with a large N-terminal portion exposed outside the cell and a short cytoplasmic domain. Computer assisted analysis shows that gp42 is distinct from previously characterized proteins, but shares a number of structural features with the class II histocompatibility antigens. The sizes of the extracellular domains of gp42 and of class II histocompatibility antigens are similar, the position of four cysteines and the location of several aa residues are conserved. Some of these conserved residues are also present in immunoglobulins (Ig) and in the neural-cell adhesion molecule, thus indicating that gp42 is a new member of the Ig superfamily.
Altruda
F
F
Dipartimento di Genetica, Biologia e Chimica Medica, Università di Torino, Italy.
Cervella
P
P
Gaeta
M L
ML
Daniele
A
A
Giancotti
F
F
Tarone
G
G
Stefanuto
G
G
Silengo
L
L
D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D030161:Avian Proteins; D001483:Base Sequence; D051926:Basigin; D001798:Blood Proteins; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D005455:Fluorescent Antibody Technique; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D007106:Immune Sera; D007136:Immunoglobulins / Q000235:genetics*; D008562:Membrane Glycoproteins / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D012689:Sequence Homology, Nucleic Acid
D015703:Antigens, CD; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D030161:Avian Proteins; D001798:Blood Proteins; C102624:Bsg protein, Gallus gallus; C497887:Bsg protein, mouse; C079432:Bsg protein, rat; D015816:Cell Adhesion Molecules, Neuronal; D000949:Histocompatibility Antigens Class II; D007106:Immune Sera; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D051926:Basigin; D004247:DNA