Tumorigenic sublethal whole-body X-irradiation of RFM mice enhances cell-mediated cytotoxicity while transiently depressing T- and B-lymphocytes.
The immunocyte composition of spleens and peritoneal exudates (PEC) from RFM mice was examined following tumorigenic doses of whole-body, sublethal X-irradiation. T-cells, B-cells and macrophages were quantitated using mAb and flow cytometry. The cell mediated cytotoxicity (CMC) potential of PEC following immunization with allogeneic tumor cells was also assayed. Although the percentages of T- and B-cells were depressed in irradiated mice, the CMC activity of PEC from these same mice was increased. Thus irradiation resulted in an increased incidence of tumors coincident with an increased CMC potential against tumor targets.
Hester
R B
RB
Department of Microbiology and Immunology, University of South Alabama, College of Medicine, Mobile 36688.
Coggin
J H
JH
D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology / Q000528:radiation effects*; D003602:Cytotoxicity, Immunologic; D005434:Flow Cytometry; D008223:Lymphoma / Q000276:immunology* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000528:radiation effects; D013601:T-Lymphocytes / Q000276:immunology / Q000528:radiation effects*; D014407:Tumor Cells, Cultured; D014916:Whole-Body Irradiation; D014965:X-Rays
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface