Characterization of purinoceptors present on human liver plasma membranes.
Using ATP alpha 35S as radioligand, we have detected the presence of specific purinoceptors on human liver plasma membranes. They are characterized by a Kd value of 0.19 microM and a Bmax of 24 pmol/mg membrane protein. These purinoceptors belong to the P2Y subclass as demonstrated by the high degree of similarity with rat liver purinoceptors, previously shown to be P2Y [(1986) Biochem. J. 240, 367-371] and known to be involved in the control of liver glycogenolysis.
Keppens
S
S
Afdeling Biochemie, Katholieke Universiteit Leuven, Belgium.
Vandekerckhove
A
A
De Wulf
H
H
D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000032:analysis*; D006801:Humans; D008099:Liver / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D011983:Receptors, Purinergic / Q000145:classification / Q000302:isolation & purification* / Q000502:physiology; D013045:Species Specificity
D011983:Receptors, Purinergic; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate