Heterogeneity of progesterone receptor expression in epithelial cells of immature and differentiating quail oviduct.
The localization of progesterone receptor (PR) in the quail oviduct was investigated before and after the onset of sexual maturation using an immunohistochemical technique. PR was revealed exclusively in nuclei of target cells whatever the hormonal state of the tissue (immature or not, pretreated or not with progesterone). In the immature or ovariectomized quail oviduct, PR was principally localized in the undifferentiated epithelial cells; some mesothelial cells and a very few stromal cells expressed the PR. Only 40-45% of the epithelial cells were immunoreactive. These positive cells were mainly localized in the furrows of the villi where further evagination of the epithelium will occur to form the tubular glands. The onset of sexual maturation was accompanied by an increase of the proportion of positive epithelial cells and stromal cells. In estradiol-treated animals, more than 90% of the tubular gland cells were strongly stained while only 40% of the luminal epithelial cells were immunoreactive. Our results show that there are two subpopulations of epithelial cells: those expressing the PR before the onset of sexual maturation even in ovariectomized quails (constitutive expression) and those expressing the PR during sexual maturation or after estrogen injection (inductive expression). These results, associated with previously published studies dealing with the cytodifferentiation of epithelial cells during natural development or after various hormonal treatments in ovariectomized animals, suggest that the first are the progenitors of tubular gland cells, and the second the progenitors of ciliated and goblet cells. In stromal cells, PR expression is also inducuible.
Pageaux
J F
JF
INSERM U 205, INSA, Villeurbanne, France.
Dufrene
L
L
Laugier
C
C
Perche
O
O
Sandoz
D
D
D000818:Animals; D003370:Coturnix; D004848:Epithelium / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D007124:Immunoenzyme Techniques; D010057:Oviducts / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D011980:Receptors, Progesterone / Q000096:biosynthesis*
D011980:Receptors, Progesterone; D004958:Estradiol