Evidence of myristylated disulfide-linked dimer of variant surface glycoprotein of Trypanosoma brucei-brucei.
1. Variant surface glycoprotein (VSGs) of Trypanosoma brucei-brucei may exist as a disulfide-linked dimer in both forms: myristylated (mfVSG) and non-myristylated (sVSG), as judge by fluorography and immunoblotting of SDS-PAGE under non-reducing conditions. 2. The dimeric VSG form is labeled with [3H]-myristic acid in our incorporation conditions. 3. AnTat 1.1 trypanosomes preincubated with tunicamycin and incubated with [3H]-myristic acid synthesized a labeled molecule that has an apparent molecular weight slightly smaller than the native form, and that also corresponds to a disulfide-linked dimer.
Hublart
M
M
Unité INSERM No. 16, Lille, France.
Mendonça-Previato
L
L
Boutignon
F
F
Huet-Duvillier
G
G
Degand
P
P
D000818:Animals; D015153:Blotting, Western; D004220:Disulfides / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D009226:Myristates / Q000032:analysis*; D009227:Myristic Acids / Q000032:analysis*; D014346:Trypanosoma brucei brucei / Q000032:analysis*; D014415:Tunicamycin / Q000494:pharmacology; D014643:Variant Surface Glycoproteins, Trypanosoma / Q000032:analysis*
D004220:Disulfides; D009226:Myristates; D009227:Myristic Acids; D014643:Variant Surface Glycoproteins, Trypanosoma; D014415:Tunicamycin