title: Production of monoclonal antibodies toward bovine interferons-alpha suitable for immunopurification.
abstract: Five murine hybridoma clones, producing monoclonal antibodies (MAbs) to bovine interferon-alpha (BoIFN-alpha) were established. One of these, F12, secreted MAbs giving high titers, in ELISA tests, neutralizing both BoIFN-alpha, -alpha C, and BoIFN-alpha D activities, belonging to the mouse IgG1 class, and having a binding affinity constant of 10(8) M-1. F12 MAbs were used for immunoaffinity purification of BoIFN-alpha, and recombinant BoIFN-alpha C from Escherichia coli extracts was purified to homogeneity in SDS-PAGE analysis and to a specific activity of 2 X 10(8) U/mg with 90% recovery of activity.
authors.affiliation: Department of Biochemistry, Israel Institute for Biological Research, Ness-Ziona.
authors.forename: D
authors.initials: D
authors.lastname: Kobiler
authors.forename: H
authors.initials: H
authors.lastname: Grosfeld
authors.forename: M
authors.initials: M
authors.lastname: Leitner
authors.forename: R
authors.initials: R
authors.lastname: Monzain
authors.forename: C
authors.initials: C
authors.lastname: Frist
authors.forename: T
authors.initials: T
authors.lastname: Seri
authors.forename: S
authors.initials: S
authors.lastname: Cohen
authors.forename: B
authors.initials: B
authors.lastname: Velan
authors.forename: A
authors.initials: A
authors.lastname: Shafferman
authors.forename: Y
authors.initials: Y
authors.lastname: Gozes
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002846:Chromatography, Affinity; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas; D007118:Immunoassay; D007074:Immunoglobulin G / Q000145:classification; D007370:Interferon Type I / Q000276:immunology*; D051379:Mice; D011994:Recombinant Proteins
chemical_list: D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007370:Interferon Type I; D011994:Recombinant Proteins