Immunological evidence for the role of phosphoprotein p68/pI = 7.3 in premessenger RNA splicing.
A 68 kDa (pI = 7.3) nuclear phosphoprotein has been previously characterized as a component of transcriptionally active chromatin. Two-dimensional PAGE Western blotting and radioimmunoassay with monoclonal antibodies have identified this protein in nuclear extracts used for in vitro RNA splicing. In vitro splicing activity could be quantitatively inhibited by preincubating nuclear extracts with the antibodies, but the assembly of 60 S spliceosomes could not.
Liew
C C
CC
Department of Clinical Biochemistry, Toronto General Hospital Research Centre, University of Toronto, Canada.
Smith
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HC
D000911:Antibodies, Monoclonal / Q000276:immunology*; D015153:Blotting, Western; D002467:Cell Nucleus / Q000032:analysis*; D002478:Cells, Cultured; D002843:Chromatin / Q000032:analysis; D053487:DEAD-box RNA Helicases; D006367:HeLa Cells; D009687:Nuclear Proteins / Q000032:analysis / Q000276:immunology*; D010750:Phosphoproteins / Q000032:analysis / Q000276:immunology*; D011494:Protein Kinases; D020365:RNA Helicases; D012326:RNA Splicing; D011863:Radioimmunoassay; D014158:Transcription, Genetic
D000911:Antibodies, Monoclonal; D002843:Chromatin; D009687:Nuclear Proteins; D010750:Phosphoproteins; D011494:Protein Kinases; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases