Studies on the mechanism of inhibition of nuclear triiodothyronine binding by fatty acids.
Studies were designed to elucidate the mechanism by which unsaturated fatty acids inhibit the binding of triiodothyronine (T3) ro rat liver nuclei. The possibility of a direct interaction between oleic acid and T3 was excluded by dialysis experiments. Oleic acid inhibits nuclear T3 binding in a strictly competitive manner. The Ki value of oleic acid was approx. 10(4) times greater than that of T3. The inhibitory effect of oleic acid could be reversed by bovine serum albumin.
van der Klis
F R
FR
Department of Experimental Endocrinology, University of Amsterdam, The Netherlands.
Wiersinga
W M
WM
de Vijlder
J J
JJ
D000818:Animals; D001667:Binding, Competitive; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D003956:Dialysis; D007700:Kinetics; D008099:Liver / Q000648:ultrastructure*; D008297:Male; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D014284:Triiodothyronine / Q000378:metabolism*
D009829:Oleic Acids; D014284:Triiodothyronine; D012710:Serum Albumin, Bovine; D019301:Oleic Acid