Automated methods for single-stranded DNA isolation and dideoxynucleotide DNA sequencing reactions on a robotic workstation.
Automated procedures have been developed for both the simultaneous isolation of 96 single-stranded M13 chimeric template DNAs in less than two hours, and for simultaneously pipetting 24 dideoxynucleotide sequencing reactions on a commercially available laboratory workstation. The DNA sequencing results obtained by either radiolabeled or fluorescent methods are consistent with the premise that automation of these portions of DNA sequencing projects will improve the reproducibility of the DNA isolation and the procedures for these normally labor-intensive steps provides an approach for rapid acquisition of large amounts of high quality, reproducible DNA sequence data.
Mardis
E R
ER
University of Oklahoma, Norman 73019.
Roe
B A
BA
D001331:Automation; D001435:Bacteriophages / Q000235:genetics*; D002213:Capsid / Q000302:isolation & purification; D011232:Chemical Precipitation; D004277:DNA, Single-Stranded / Q000302:isolation & purification*; D004279:DNA, Viral / Q000302:isolation & purification*; D004259:DNA-Directed DNA Polymerase; D015224:Dideoxynucleosides; D004586:Electrophoresis / Q000379:methods; D004926:Escherichia coli; D005453:Fluorescence; D009841:Oligonucleotides / Q000235:genetics; D010761:Phosphorus Radioisotopes; D012371:Robotics; D013698:Templates, Genetic
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D015224:Dideoxynucleosides; D009841:Oligonucleotides; D010761:Phosphorus Radioisotopes; D004259:DNA-Directed DNA Polymerase