[The characteristics of the chemical composition and biological activity of Proteus antigens isolated using hydroxylamine].
The comparative study of the chemical composition and biological properties of antigens isolated from Proteus vulgaris with the use of hydroxylamine and by two classical methods (Boivin's and Westphal's methods) has been made. As shown in this study, the treatment of bacteria with hydroxylamine makes it possible to obtain antigenic complexes with lower toxicity. At the same time hydroxylamine produces no denaturing effect on lipopolysaccharides and protein fractions of bacterial cells.
Kaverina
K G
KG
Krasnikova
I N
IN
Levina
L A
LA
Solov'eva
T F
TF
Kreĭnin
L S
LS
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification / Q000633:toxicity; D055598:Chemical Phenomena; D002621:Chemistry; D019811:Hydroxylamine; D006898:Hydroxylamines; D007114:Immunization; D007158:Immunologic Techniques; D007202:Indicators and Reagents; D007928:Lethal Dose 50; D008070:Lipopolysaccharides / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification / Q000633:toxicity; D051379:Mice; D011514:Proteus vulgaris / Q000187:drug effects / Q000276:immunology*
D000942:Antigens, Bacterial; D006898:Hydroxylamines; D007202:Indicators and Reagents; D008070:Lipopolysaccharides; D019811:Hydroxylamine
Kharakteristika khimicheskogo sostava i biologicheskoĭ aktivnosti antigenov proteia, izolirovannykh s pomoshch'iu gidroksilamina.