Effects of progesterone and oestradiol-17 beta on 17 beta-ol-dehydrogenase activity in stromal cells of human endometrium under in-vitro conditions.
A 17 beta-ol-dehydrogenase activity could be demonstrated in fibroblast monolayer cell cultures of proliferative human endometrium. After 24 h incubation 100 nCi [6,7-3H]oestradiol-17 beta was completely oxidized to oestrone. Progesterone was not able to enhance the metabolizing velocity. In contrast, progesterone incubation revealed a decreasing oxidation rate with increasing molarity. Histological changes after transformation of the endometrium are discussed to explain in-vivo results showing an increased 17 beta-ol-dehydrogenase activity in the secretory phase of the cycle.
Neulen
J
J
Department of Obstetrics and Gynaecology, University of Freiburg, FRG.
Wagner
B
B
Flecken
U
U
Breckwoldt
M
M
D015067:17-Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D002478:Cells, Cultured; D004717:Endometrium / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D004958:Estradiol / Q000378:metabolism / Q000494:pharmacology*; D004960:Estradiol Dehydrogenases / Q000378:metabolism*; D004970:Estrone / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D008597:Menstrual Cycle; D011374:Progesterone / Q000494:pharmacology*
D004970:Estrone; D011374:Progesterone; D004958:Estradiol; D015067:17-Hydroxysteroid Dehydrogenases; D004960:Estradiol Dehydrogenases