Quick-freeze, deep-etch visualization of the nuclear pore complex.
We have visualized the nuclear pore complex (NPCx) three-dimensionally by quick-freeze, deep etching and suggest that NPCx is composed of four rings and associated filaments. The rings were located at the cytoplasmic side, at the nucleoplasmic side, and at the pore waist as well. Some of the filaments emanated toward the cytoplasm, some sprawled over the cytoplasmic face of the nuclear membrane, and some intervened the rings. The emanating filaments were considered to be vimentin filaments judging from the replica and thin-sectioned image.
Fujitani
Y
Y
Higaki
S
S
Sawada
H
H
Hirosawa
K
K
D005613:Freeze Etching; D006801:Humans; D008854:Microscopy, Electron; D009685:Nuclear Envelope / Q000648:ultrastructure*; D012516:Osteosarcoma; D014407:Tumor Cells, Cultured