[Biosynthesis of somatotropin in a recombinant strain of Escherichia coli].
A recombinant Escherichia coli K-12 strain was grown in the regime of chemostat with glucose limitation at a different flow rate and in the regime of turbidostat. The stability of its population and the dynamics of somatotropin biosynthesis were studied. The plasmid-containing strain became less stable as the flow rate in the fermenter dropped down, which was due, apparently, to a greater limitation. The level of somatotropin biosynthesis was higher at a low dilution rate (D = 0.075, 0.17 and 0.34 h-1). Possible factors responsible for this phenomenon are discussed.
Gorlatova
N V
NV
Eroshina
N V
NV
Anan'in
V M
VM
Baryshnikova
L M
LM
Akimenko
L V
LV
D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D013006:Growth Hormone / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D011995:Recombination, Genetic
D011994:Recombinant Proteins; D013006:Growth Hormone
Biosintez somatotropina rekombinantnym shtammom Escherichia coli.