[Possible activation of human prorenin by contact with the cell membrane].
Semipurified human kidney prorenin was exposed in vitro to a mixture of lipids mimicking the composition of the inner leaflet of the cell membrane, in the presence of semipurified human angiotensinogen at a concentration of 1/4 Km. Prorenin was activated in a time-dependent manner over a period of 60 min. This lipid-dependent activation was completely reversed thereafter. Pre-incubation with anti-renin serum completely prevented this activated prorenin-dependent generation of angiotensin I. Our data suggest that human prorenin can be reversibly activated by contact with the cell membrane.
Troffa
C
C
Tonolo
G
G
Manunta
P
P
Pazzola
A
A
Melis
M G
MG
Pala
F
F
Soro
A
A
Madeddu
P
P
Glorioso
N
N
D000803:Angiotensin I / Q000096:biosynthesis*; D002462:Cell Membrane / Q000378:metabolism*; D004792:Enzyme Precursors / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D050356:Lipid Metabolism; D012083:Renin / Q000378:metabolism*
D004792:Enzyme Precursors; D000803:Angiotensin I; D012083:Renin
Possibile attivazione di contatto della prorenina umana con la membrana cellulare.