title: Application of solid-phase antibodies to radioimmunoassay. Improved performance of Dynospheres following affinity purification of antisera.
abstract: Affinity purification of antibody before covalent binding to pre-activated micro-particles (Dynospheres) was necessary to overcome the reduced coupling capacity evident with low titre antisera, particularly antihapten sera. Optimally prepared Dynospheres anti-thyroxine provided a radioimmunoassay with good performance and economical usage of microparticles. Assay sensitivity was less than 10 nmol/l while precision across the clinically relevant dose range (50-150 nmol/l) was 3% CV using 125 micrograms particles per assay tube.
authors.affiliation: Institute of Biochemistry, Royal Infirmary, Glasgow, UK.
authors.forename: M G
authors.initials: MG
authors.lastname: McConway
authors.forename: R S
authors.initials: RS
authors.lastname: Chapman
mesh_terms: D000937:Antigen-Antibody Reactions; D002846:Chromatography, Affinity; D007074:Immunoglobulin G / Q000276:immunology / Q000302:isolation & purification*; D011863:Radioimmunoassay / Q000379:methods*; D013974:Thyroxine / Q000276:immunology*
chemical_list: D007074:Immunoglobulin G; D013974:Thyroxine