Rapid screening of organosulfur agents for potential chemopreventive activity using the murine NA assay.
The chemopreventive efficacy of 11 organosulfur compounds was assessed using the murine nuclear aberration (NA) assay in C57BL/6J mice. The sulfur compounds were introduced by stomach gavage. Benzo[a]pyrene (BP), which is a carcinogen known to a) undergo biotransformation by pathways mediated by P-450 and b) induce NA in the intestine, was used as the challenge. All animals were killed 48 hours after BP injection, and NA per crypt were scored. The results indicated that several agents were active in inhibiting BP nucleotoxicity to the colon, most notably, allyl mercaptan, benzyl mercaptan, and phenylethyl mercaptan. The NA assay was useful in effectively prescreening certain compounds for potential interactions with chemical carcinogens, thus serving as one indicator of chemopreventive activity.
Wargovich
M J
MJ
Department of Medical Oncology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Eng
V W
VW
D000818:Animals; D000970:Antineoplastic Agents; D001564:Benzo(a)pyrene / Q000633:toxicity; D003106:Colon / Q000648:ultrastructure; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D015162:Micronucleus Tests; D013438:Sulfhydryl Compounds / Q000494:pharmacology*
D000970:Antineoplastic Agents; D013438:Sulfhydryl Compounds; D001564:Benzo(a)pyrene