In vitro inhibition of lens epithelial cell growth by continuous wave Nd:YAG laser.
Bovine lens epithelial cells were suspended in MEM medium and subjected to continuous wave, low power, pulsed neodymium:yttrium-aluminum-garnet (Nd:YAG) laser irradiation. The temperature of each suspension was maintained at 36 degrees C. Laser applications ranged from 1 to 10 watts and from 100 to 2000 seconds, but the total dose to each of the epithelial cell suspension was 2000 J. Six to thirty-nine percent of the cells were dead immediately after irradiation. Surviving cells, cultured for 15 days, showed decreased attachment and failed to grow. These preliminary results suggest that the Nd:YAG laser may be used during cataract surgery to prevent subsequent lens epithelial cell proliferation and the resulting vision reduction and glare.
Miyake
K
K
Shohzankai Medical Foundation, Miyake Eye Hospital, Nagoya, Japan.
Iwata
S
S
Ando
F
F
Daikuzono
N
N
Federman
J L
JL
D000818:Animals; D002455:Cell Division / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D004847:Epithelial Cells; D007834:Lasers; D007908:Lens, Crystalline / Q000166:cytology*