Evidence that the enoyl-CoA hydratase bifunctional protein of mouse liver peroxisomes is identical with the 70,000 dalton peroxisomal membrane protein.
Peroxisomal enoyl-CoA hydratase was purified from livers of mice treated with di-(2-ethylhexyl)phthalate and its properties compared with those of the 70 kDa protein present in the membranes prepared by carbonate extraction of peroxisomes. The two proteins had identical subunit molecular masses, of about 70,000 daltons. Limited proteolysis of these proteins using the V8 proteinase of S. aureus yielded identical peptide maps, with these peptides crossreacting with antiserum raised against the 70 kDa membrane protein. These data are consistent with the proposal that the peroxisomal 70 kDa membrane protein and the peroxisomal enoyl-CoA hydratase are the same protein.
Crane
D I
DI
Division of Science and Technology, Griffith University, Nathan, Australia.
Chen
N H
NH
Masters
C J
CJ
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D004746:Enoyl-CoA Hydratase / Q000302:isolation & purification*; D005260:Female; D006836:Hydro-Lyases / Q000302:isolation & purification*; D015151:Immunoblotting; D007425:Intracellular Membranes / Q000032:analysis*; D008099:Liver / Q000201:enzymology*; D008565:Membrane Proteins / Q000302:isolation & purification*; D051379:Mice; D008815:Mice, Inbred Strains; D008830:Microbodies / Q000201:enzymology*; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000302:isolation & purification*; D010449:Peptide Mapping; D013388:Succinimides
D008565:Membrane Proteins; D009097:Multienzyme Complexes; D013388:Succinimides; C015752:N-chlorosuccinimide; D006836:Hydro-Lyases; D004746:Enoyl-CoA Hydratase