Amino acid uptake and incorporation not cell-specific peptides and evidence for intracellular peptide pools in Aplysia neurons R3-R14.
1. Relationships between intracellular amino acid concentrations and uptake rates and their utilization in synthesis of cell-specific peptides in neurons R3-R14 in the Aplysia parietovisceral ganglion are explored. 2. The uptake rates and intracellular concentrations of most amino acids are positively correlated and inversely related to their degree of incorporation into the peptides. 3. The bulk cellular pool of arginine is probably utilized in the synthesis of R3-R14 peptides, but much of the glycine taken up appears not to be readily available for protein synthesis. 4. There are rapidly and slowly turning over pools of the peptides, and portions of the peptides stay in the cell bodies for days.
Lin
C Y
CY
Marine Biomedical Institute, University of Texas Medical Branch, Galveston 77550-2772.
Hughes
M G
MG
McAdoo
D J
DJ
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001048:Aplysia / Q000378:metabolism*; D001693:Biological Transport, Active; D005724:Ganglia / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D007424:Intracellular Fluid / Q000378:metabolism; D008970:Molecular Weight; D009474:Neurons / Q000378:metabolism*; D009479:Neuropeptides / Q000378:metabolism*
D000596:Amino Acids; D009479:Neuropeptides; D005998:Glycine