Selenium effects on glutathione peroxidase and the immune response of stressed calves challenged with Pasteurella hemolytica.
The present study was conducted to determine whether a marginal Se deficiency affects health, blood characteristics and the immune response of calves subjected to stresses associated with weaning, shipping (332 km) and Pasteurella hemolytica inoculation. Treatments were 1) -Se, 2) -Se/P. hemolytica, 3) +Se (.1 mg Se/kg feed) and 4) +Se/P. hemolytica. Previous Se intake was controlled; dams of -Se calves were fed diets marginally deficient in Se (.03 to .05 mg/kg), whereas dams of +Se calves received a s.c. injection of 30 mg Se (as sodium selenite) every 60 d. Calves were inoculated with P. hemolytica intratracheally on d 3 following weaning and transport. Inoculation with P. hemolytica increased (P less than .05) body temperatures, platelet counts, serum IgM concentrations and serum antibody titers and decreased serum albumin concentrations at 4 to 7 d postinoculation. Weight gains for the 21-d study were not affected by Se status, although whole blood and plasma glutathione peroxidase (GSH-Px) were higher (P less than .05) for +Se calves. Plasma GSH-Px increased (P less than .01) in calves showing signs of morbidity. Increases in plasma GSH-Px were correlated positively with body temperature. Serum IgM concentrations were higher (P less than .05) in +Se calves on d 17, but Se-supplemented calves had lower (P less than .05) anti-P. hemolytica titers on d 17 than -Se calves. Selenium status did not affect body temperatures, plasma creatine phosphokinase or serum IgG and albumin concentrations. These results indicate that Se status can affect IgM concentrations following stress.
Stabel
J R
JR
North Carolina State University, Raleigh 27695-7621.
Spears
J W
JW
Brown
T T
TT
Brake
J
J
D000818:Animals; D002417:Cattle / Q000276:immunology*; D002418:Cattle Diseases / Q000201:enzymology / Q000276:immunology / Q000382:microbiology / Q000503:physiopathology*; D005979:Glutathione Peroxidase / Q000097:blood*; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D010326:Pasteurella Infections / Q000201:enzymology / Q000276:immunology / Q000662:veterinary*; D012643:Selenium / Q000172:deficiency / Q000494:pharmacology*; D013312:Stress, Physiological / Q000276:immunology / Q000503:physiopathology / Q000662:veterinary*; D015430:Weight Gain / Q000187:drug effects
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D005979:Glutathione Peroxidase; D012643:Selenium