title: Pre-S proteins in hepatitis B.
abstract: Two reactive sequences of the pre-S regions of hepatitis B surface antigen were synthesized chemically and used in micro-ELISAs for the assay of pre-S1 and pre-S2 antigens in serum from patients with acute and chronic hepatitis B. Pre-S1 antigen correlated well with the presence of HBV-DNA and was no longer detectable on cessation of viral replication, after natural recovery and after successful treatment with alpha-interferon. Pre-S2 proteins were also lost after treatment with alpha-interferon. The results show that the assay of pre-S1 and pre-S2 proteins in serum provides additional useful markers for assessing patients with acute and chronic hepatitis B infection and for monitoring the response to treatment with interferon.
authors.affiliation: Scientific Development Group, Organon International BV, Oss, Netherlands.
authors.forename: L
authors.initials: L
authors.lastname: Kuijpers
authors.forename: M
authors.initials: M
authors.lastname: Koens
authors.forename: I
authors.initials: I
authors.lastname: Murray-Lyon
authors.forename: J C
authors.initials: JC
authors.lastname: Coleman
authors.forename: P
authors.initials: P
authors.lastname: Karayiannis
authors.forename: H C
authors.initials: HC
authors.lastname: Thomas
authors.forename: A
authors.initials: A
authors.lastname: Zanetti
authors.forename: A
authors.initials: A
authors.lastname: Cargnel
authors.forename: T J
authors.initials: TJ
authors.lastname: Harrison
authors.forename: A J
authors.initials: AJ
authors.lastname: Zuckerman
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D004279:DNA, Viral / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D006509:Hepatitis B / Q000175:diagnosis* / Q000628:therapy; D006510:Hepatitis B Antibodies / Q000096:biosynthesis; D006514:Hepatitis B Surface Antigens / Q000032:analysis / Q000138:chemical synthesis* / Q000276:immunology; D006515:Hepatitis B virus / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology / Q000627:therapeutic use; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011498:Protein Precursors / Q000032:analysis / Q000138:chemical synthesis* / Q000276:immunology; D014759:Viral Envelope Proteins / Q000032:analysis / Q000138:chemical synthesis* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D004279:DNA, Viral; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D007370:Interferon Type I; D011498:Protein Precursors; D014759:Viral Envelope Proteins; C050806:presurface protein 1, hepatitis B surface antigen; C050807:presurface protein 2, hepatitis B surface antigen