Immunoadjuvant action of liposomes: comparison with other adjuvants.
Dehydration-rehydration vesicles (DRV liposomes) composed of equimolar phospholipid and cholesterol and containing bovine serum albumin (BSA) were used together with free BSA to immunize Balb/C mice. Primary and secondary immune responses (IgG1) to the liposomal antigen, as measured by ELISA in mouse sera, were similar for egg phosphatidylcholine (PC) and distearoyl phosphatidylcholine (DSPC) DRV, and much greater than those elicited by free BSA. The adjuvanticity of PC DRV was compared with that of aluminium salts (alum), complete Freund's adjuvant (CFA) and N-acetyl muramyl-L-threonyl-D-isoglutamine ([Thr1]MDP), the latter used as such or in a liposome form co-entrapped with the antigen. DRV (with or without co-entrapped [Thr1]MDP), and alum were equally strong in producing primary and secondary immune responses (IgG1) to BSA. Such responses were significantly higher than those achieved with CFA and [Thr1]MDP alone. The implications of these results for the potential role of liposomes as immunological adjuvants in vaccines are discussed.
Gregoriadis
G
G
Academic Department of Medicine, Royal Free Hospital, School of Medicine, London, U.K.
Panagiotidi
C
C
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000378:metabolism; D000276:Adjuvants, Immunologic / Q000276:immunology*; D000534:Alum Compounds / Q000378:metabolism; D000818:Animals; D000917:Antibody Formation; D004797:Enzyme-Linked Immunosorbent Assay; D005620:Freund's Adjuvant / Q000378:metabolism; D008081:Liposomes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010713:Phosphatidylcholines / Q000378:metabolism; D013997:Time Factors
D000276:Adjuvants, Immunologic; D000534:Alum Compounds; D008081:Liposomes; D010713:Phosphatidylcholines; D000119:Acetylmuramyl-Alanyl-Isoglutamine; D005620:Freund's Adjuvant