Pre-S proteins in hepatitis B.
Two reactive sequences of the pre-S regions of hepatitis B surface antigen were synthesized chemically and used in micro-ELISAs for the assay of pre-S1 and pre-S2 antigens in serum from patients with acute and chronic hepatitis B. Pre-S1 antigen correlated well with the presence of HBV-DNA and was no longer detectable on cessation of viral replication, after natural recovery and after successful treatment with alpha-interferon. Pre-S2 proteins were also lost after treatment with alpha-interferon. The results show that the assay of pre-S1 and pre-S2 proteins in serum provides additional useful markers for assessing patients with acute and chronic hepatitis B infection and for monitoring the response to treatment with interferon.
Kuijpers
L
L
Scientific Development Group, Organon International BV, Oss, Netherlands.
Koens
M
M
Murray-Lyon
I
I
Coleman
J C
JC
Karayiannis
P
P
Thomas
H C
HC
Zanetti
A
A
Cargnel
A
A
Harrison
T J
TJ
Zuckerman
A J
AJ
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D004279:DNA, Viral / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D006509:Hepatitis B / Q000175:diagnosis* / Q000628:therapy; D006510:Hepatitis B Antibodies / Q000096:biosynthesis; D006514:Hepatitis B Surface Antigens / Q000032:analysis / Q000138:chemical synthesis* / Q000276:immunology; D006515:Hepatitis B virus / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology / Q000627:therapeutic use; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011498:Protein Precursors / Q000032:analysis / Q000138:chemical synthesis* / Q000276:immunology; D014759:Viral Envelope Proteins / Q000032:analysis / Q000138:chemical synthesis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D004279:DNA, Viral; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D007370:Interferon Type I; D011498:Protein Precursors; D014759:Viral Envelope Proteins; C050806:presurface protein 1, hepatitis B surface antigen; C050807:presurface protein 2, hepatitis B surface antigen