title: The influence of perimenopausal age on estrogen receptor status and estradiol and DNA content in human breast cancer.
abstract: In 25 cases of breast cancer occurring in perimenopausal age, estrogen receptor (ER) content was determined by the dextran-coated charcoal (DCC) assay and both endogenous-bound estradiol and nuclear DNA concentrations were measured by computerized quantitative analysis on formalin-fixed paraffin-embedded tissue samples. No statistically significant relationship (p greater than 0.05) was found within these parameters. The high incidence (64%) of ER-negative cases in this menopausal age was mainly due (62.5%) to interference of high levels of endogenous estradiol occupying the receptor sites in-vivo. The prevalence of hormone-insensitive and aneuploid cell clones accounted for the remaining true ER-negative tumours (37.5%).
authors.affiliation: Department of Pathology - City Hospital, Alessandria, Italy.
authors.forename: P G
authors.initials: PG
authors.lastname: Betta
authors.forename: F
authors.initials: F
authors.lastname: Robutti
authors.forename: G
authors.initials: G
authors.lastname: Spinoglio
authors.forename: G
authors.initials: G
authors.lastname: Bottero
mesh_terms: D000367:Age Factors; D001943:Breast Neoplasms / Q000032:analysis* / Q000188:drug therapy; D004247:DNA / Q000032:analysis*; D004958:Estradiol / Q000032:analysis* / Q000502:physiology; D005260:Female; D006801:Humans; D008593:Menopause; D008875:Middle Aged; D011003:Ploidies / Q000187:drug effects; D011960:Receptors, Estrogen / Q000032:analysis* / Q000502:physiology
chemical_list: D011960:Receptors, Estrogen; D004958:Estradiol; D004247:DNA