The unequivocal determination of lactic acid using a one-dimensional zero-quantum coherence-transfer technique.
A method based on zero-quantum coherence transfer for spectral editing of metabolites in aqueous solution in a one-dimensional experiment is described. Water suppression factors of approximately 8000 were achieved by the use of pulsed B0 field gradients and excellent editing was obtained in a single acquisition. The methyl resonances of both lactate and acetaldehyde were readily observed in a mouse brain homogenate by generating zero-quantum coherence using Gaussian pulses for selective excitation of the respective CH resonances.
Doddrell
D M
DM
Department of Chemistry, University of Queensland, St. Lucia, Australia.
Brereton
I M
IM
Moxon
L N
LN
Galloway
G J
GJ
D000447:Aldehydes / Q000378:metabolism; D000818:Animals; D001834:Body Water; D001921:Brain / Q000378:metabolism; D007773:Lactates / Q000032:analysis*; D019344:Lactic Acid; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D051379:Mice; D008954:Models, Biological; D014867:Water
D000447:Aldehydes; D007773:Lactates; D014867:Water; D019344:Lactic Acid