Phospholipid, nature's own slide and cover slip for cryo-electron microscopy.
Thin films of surface-active compounds, with or without particulate material, can be obtained by immersing and withdrawing a bare specimen grid from a solution/suspension of the compound. Immediately after withdrawing the grid, thinning of the film starts. Thinning is initially powered by gravity and capillary forces and will proceed in thin films (less than 100 nm) driven by intermolecular forces until the London-van der Waals attractive forces come to an equilibrium with electrostatic repulsion of similarly charged surfaces of the film. With small unilamellar vesicles prepared from the phospholipid dimyristoyl phosphatidyl choline (DMPC) the draining behaviour of these films was studied by cryo-electron microscopy. Small unilamellar vesicles were observed within the film as well as the coalescence of these vesicles into sheets ('leaky' membrane fusion). Sheets dominate the images when films are allowed to drain for longer periods (greater than 3 min). Thin films were formed on grids from catalase crystals suspended in a DMPC suspension and vitrified by cooling. High-resolution information was obtained by electron diffraction at low temperature and under low-dose conditions from catalase crystals surrounded by small vesicles as well as from catalase crystals surrounded by sheets of DMPC. In the latter case the water content drops from 99% (DMPC in small vesicles) to less than 30% (DMPC in sheets) during draining. Ferritin was added to a DMPC suspension and thin films were prepared and vitrified. After prolonged draining ferritin molecules were deposited in layers with a stepwise increase in thickness. Draining of thin films has thus a dehydrating effect as well as a sorting and ordering effect. These effects must be considered when using surface-active compounds at air-water interfaces as a slide and cover slip for electron microscopy.
Frederik
P M
PM
Department of Pathology, University of Limburg, Maastricht, The Netherlands.
Stuart
M C
MC
Bomans
P H
PH
Busing
W M
WM
D002374:Catalase; D003720:Densitometry; D004134:Dimyristoylphosphatidylcholine; D005293:Ferritins; D005615:Freezing; D008854:Microscopy, Electron / Q000379:methods*; D008855:Microscopy, Electron, Scanning
D005293:Ferritins; D002374:Catalase; D004134:Dimyristoylphosphatidylcholine