Determination of partial amino acid sequence of lipoate acetyltransferase of rat pyruvate dehydrogenase complex.
The partial amino acid sequence of rat lipoate acetyltransferase was determined using the intact protein and the peptides derived from a digest with Achromobacter protease I. The results showed the amino-terminal sequence of the mature enzyme to be (N) Ser-Leu-Pro-Pro-His-Gln-Lys-Val-Pro-Leu-Pro-Ser- Leu-Ser-Pro-Thr-Met-Gln-Ala-Gly-Thr-Ile-Ala-Arg-Trp-Glu-Lys. In addition, the sequences of two possible lipoyl-binding sites in the subunit, which are very similar to each other, were established.
Matuda
S
S
Department of Biology, Kanoya National Institute of Fitness and Sports, Kagoshima, Japan.
Nakano
K
K
Saheki
T
T
Oyama
R
R
Suzuki
M
M
Titani
K
K
D000123:Acetyltransferases / Q000032:analysis / Q000378:metabolism; D000421:Alcaligenes / Q000201:enzymology; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D051047:Dihydrolipoyllysine-Residue Acetyltransferase; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000378:metabolism; D011768:Pyruvate Dehydrogenase Complex; D051381:Rats; D012689:Sequence Homology, Nucleic Acid
D000596:Amino Acids; D011768:Pyruvate Dehydrogenase Complex; D000123:Acetyltransferases; D051047:Dihydrolipoyllysine-Residue Acetyltransferase; D010447:Peptide Hydrolases