Synthesis and secretion of phospholipase C by oral spirochetes.
Four strains of oral treponemes and Treponema phagedenis Reiter synthesize and secrete phospholipase C (PLC), which was detected by the hydrolysis of p-nitrophenylphosphorylcholine. PLC was detected in gingival crevicular fluid from diseased but not from healthy sulci. The initiation and progression of periodontal lesions may begin with the hydrolysis of membrane phospholipids by PLC.
Siboo
R
R
Department of Microbiology and Immunology, Faculty of Medicine, McGill University, Montreal, Quebec, Canada.
al-Joburi
W
W
Gornitsky
M
M
Chan
E C
EC
D002794:Choline / Q000031:analogs & derivatives*; D006801:Humans; D006868:Hydrolysis; D010518:Periodontitis / Q000382:microbiology*; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000378:metabolism; D014208:Treponema / Q000201:enzymology*; D010738:Type C Phospholipases / Q000096:biosynthesis* / Q000378:metabolism
C033523:4-nitrophenylphosphorylcholine; D010767:Phosphorylcholine; D010738:Type C Phospholipases; D002794:Choline