Isolation of neurosecretory granules containing vasotocin, mesotocin, MSEL- and VLDV-neurophysins from goose neurohypophysis.
Neurosecretory granules have been isolated from goose posterior pituitaries and their contents have been analyzed by reverse-phase high-pressure liquid chromatography and polyacrylamide gel electrophoresis. Vasotocin and mesotocin have been identified by their biological activities and their retention times compared with those of synthetic peptides. MSEL- and VLDV-neurophysins have been characterized by their N-terminal sequences, their electrophoretic migrations and their retention times, compared with those of purified goose neurophysins. In contrast to the two-step processing of mammalian provasopressin, processing of the vasotocin - MSEL-neurophysin precursor appears to involve only one cleavage giving the hormone and a "big" MSEL-neurophysin homologous to mammalian MSEL-neurophysin extended by copeptin.
Rouillé
Y
Y
Laboratory of Biological Chemistry, University of Paris VI, France.
Lévy
B
B
Chauvet
M T
MT
Chauvet
J
J
Acher
R
R
D000818:Animals; D001127:Arginine Vasopressin / Q000032:analysis; D002458:Cell Fractionation; D002851:Chromatography, High Pressure Liquid; D003594:Cytoplasmic Granules / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005777:Geese / Q000378:metabolism*; D009479:Neuropeptides / Q000032:analysis*; D009481:Neurophysins / Q000032:analysis; D010121:Oxytocin / Q000031:analogs & derivatives / Q000032:analysis; D010904:Pituitary Gland, Posterior / Q000032:analysis*; D011498:Protein Precursors / Q000032:analysis; D014668:Vasotocin / Q000032:analysis
D009479:Neuropeptides; D009481:Neurophysins; D011498:Protein Precursors; D001127:Arginine Vasopressin; C001713:mesotocin; D010121:Oxytocin; C055597:VLDV neurophysin; D014668:Vasotocin