A non-radioactive DNA probe for the detection of Mycoplasma pulmonis in murine mycoplasmosis.
A non-radioactive DNA probe for the detection of Mycoplasma pulmonis was developed by using photobiotin. The probe detected 0.3 ng chromosomal DNA of M. pulmonis. No cross-hybridization was observed between the probe and the other murine mycoplasma species, M. arthritidis and M. neurolyticum.
Matsuzaki
M
M
Department of Veterinary Microbiology, Faculty of Agriculture, Miyazaki University.
Harasawa
R
R
Kimizuka
F
F
Koshimizu
K
K
D000818:Animals; D001386:Azides; D001710:Biotin / Q000031:analogs & derivatives; D015139:Blotting, Southern; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D051379:Mice; D009174:Mycoplasma / Q000235:genetics / Q000302:isolation & purification*; D009175:Mycoplasma Infections / Q000175:diagnosis*
D001386:Azides; D015342:DNA Probes; D004269:DNA, Bacterial; D001710:Biotin; C045549:photobiotin