Polyamine metabolism in Setaria cervi, the bovine filarial worm.
Spermine and spermidine were found to be the principal polyamines in the bovine filarial parasite Setaria cervi, whereas putrescine was observed in very low amounts. Studies conducted on the enzymes of polyamine biosynthesis revealed low activity for S-adenosyl-methionine decarboxylase, questionable and negligible activities for the decarboxylation of ornithine and arginine, and appreciable activity for ornithine aminotransferase. Uptake studies with radiolabeled putrescine, spermidine and spermine showed that these amines are rapidly taken up from the medium by an active uptake process. The uptake was temperature-sensitive and abolished at 0-4 degrees C. The questionable presence of biosynthetic enzymes such as ornithine and arginine decarboxylase and, on the other hand, an effective uptake mechanism indicate that the parasite may depend on the host for its polyamine requirement, thereby indicating a possible target for chemotherapy.
Singh
R P
RP
Biochemistry Division, Central Drug Research Institute, Chattar Manzil, Lucknow, India.
Saxena
J K
JK
Ghatak
S
S
Shukla
O P
OP
Wittich
R M
RM
Walter
R D
RD
D012437:Adenosylmethionine Decarboxylase / Q000378:metabolism; D000818:Animals; D002020:Buffaloes; D002851:Chromatography, High Pressure Liquid; D005370:Filarioidea / Q000032:analysis / Q000201:enzymology / Q000378:metabolism*; D009955:Ornithine Decarboxylase / Q000378:metabolism; D009953:Ornithine-Oxo-Acid Transaminase / Q000378:metabolism; D011073:Polyamines / Q000032:analysis / Q000378:metabolism*; D011700:Putrescine / Q000032:analysis / Q000378:metabolism; D012719:Setariasis / Q000469:parasitology; D013095:Spermidine / Q000032:analysis / Q000378:metabolism; D013096:Spermine / Q000032:analysis / Q000378:metabolism
D011073:Polyamines; D013096:Spermine; D009953:Ornithine-Oxo-Acid Transaminase; D009955:Ornithine Decarboxylase; D012437:Adenosylmethionine Decarboxylase; D013095:Spermidine; D011700:Putrescine