title: An automated procedure for measuring biotinidase activity in serum.
abstract: In this automated procedure for quantifying biotinidase activity in human serum, a manual colorimetric method that measures conversion of the enzyme's artificial substrate N-biotinyl p-aminobenzoate was modified for use with a Technicon AutoAnalyzer II. The intra-run replicate precision (CV) was 2.1% and the day-to-day CV was 4.6% for quality-control sera. Results were linearly related to biotinidase activity in serum over the complete range of clinically relevant values, 0.2 to 11.0 U/L. Moreover, results of the automated assay were not significantly different from those of the manual assay. Because the automated procedure is faster and more precise, we recommend it for population-based studies and some screening studies.
authors.affiliation: Department of Human Genetics, Medical College of Virginia of Virginia Commonwealth University, Richmond 23298-0033.
authors.forename: K A
authors.initials: KA
authors.lastname: Weissbecker
authors.forename: H D
authors.initials: HD
authors.lastname: Gruemer
authors.forename: G S
authors.initials: GS
authors.lastname: Heard
authors.forename: W G
authors.initials: WG
authors.lastname: Miller
authors.forename: W E
authors.initials: WE
authors.lastname: Nance
authors.forename: B
authors.initials: B
authors.lastname: Wolf
mesh_terms: D000581:Amidohydrolases / Q000097:blood*; D001322:Autoanalysis / Q000295:instrumentation; D043523:Biotinidase; D003124:Colorimetry; D006801:Humans
chemical_list: D000581:Amidohydrolases; D043523:Biotinidase