title: Protein associated with the sensory cell layer of the rainbow trout saccular macula.
abstract: A protein has been detected that is associated with the saccular hair cell layer of the rainbow trout, Salmo gairdnerii R. By one- and two-dimensional SDS polyacrylamide gel electrophoresis, the molecular weight and isoelectric point of this protein are estimated to be 13.6 and 8.8 kDa, respectively. The 13.6 kDa protein cannot be detected electrophoretically in brain, gill, liver, and fractions containing the basal lamina, non-sensory epithelium, and saccular nerve. This protein does not bind antibodies to bovine myelin basic protein, while trout myelin basic proteins in the same molecular weight range do. In addition, the protein does not bind concanavalin A or react with the periodic acid-Schiff reagent. The 13.6 kDa band represents about 1% of the total protein in saccular sensory epithelium, and may be a marker protein for the hair cell layer.
authors.affiliation: Department of Otolaryngology, Wayne State University School of Medicine, Detroit, MI 48201.
authors.forename: D G
authors.initials: DG
authors.lastname: Drescher
authors.forename: K M
authors.initials: KM
authors.lastname: Khan
authors.forename: R L
authors.initials: RL
authors.lastname: Arden
authors.forename: M J
authors.initials: MJ
authors.lastname: Drescher
authors.forename: T P
authors.initials: TP
authors.lastname: Kerr
authors.forename: J S
authors.initials: JS
authors.lastname: Hatfield
mesh_terms: D000818:Animals; D006198:Hair Cells, Auditory / Q000032:analysis* / Q000166:cytology; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000302:isolation & purification*; D012487:Salmonidae / Q000378:metabolism*; D013347:Subcellular Fractions / Q000032:analysis; D014337:Trout / Q000378:metabolism*
chemical_list: D009419:Nerve Tissue Proteins