title: High homology between 6-aminohexanoate-cyclic-dimer hydrolases of Flavobacterium and Pseudomonas strains.
abstract: The nucleotide sequences of the genes for 6-aminohexanoate-cyclic-dimer hydrolases of Flavobacterium sp. strain K172 (F-nylA) and Pseudomonas sp. NK87 (P-nylA), enzymes essential for the degradation of a by-product of the nylon-6 industry, were obtained by the dideoxynucleotide chain-termination method. A 1,479-base-pair open reading frame starting at a GTG and terminating at a TGA was found for the both of the genes. The P-nylA and F-nylA genes encoded polypeptides of 493 amino acids and had only 10 base substitutions in the coding region, which caused seven amino acid substitutions.
authors.affiliation: Department of Fermentation Technology, Osaka University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Tsuchiya
authors.forename: S
authors.initials: S
authors.lastname: Fukuyama
authors.forename: N
authors.initials: N
authors.lastname: Kanzaki
authors.forename: K
authors.initials: K
authors.lastname: Kanagawa
authors.forename: S
authors.initials: S
authors.lastname: Negoro
authors.forename: H
authors.initials: H
authors.lastname: Okada
mesh_terms: D000581:Amidohydrolases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D005417:Flavobacterium / Q000201:enzymology* / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D011549:Pseudomonas / Q000201:enzymology* / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D000581:Amidohydrolases; C019683:6-aminohexanoate-cyclic-dimer hydrolase