Immunological detection of penicillin-binding protein 2' in clinical isolates of methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis.
The additional penicillin-binding protein (PBP 2') that is important in determining intrinsic resistance in methicillin-resistant strains of Staphylococcus aureus (MRSA) has been detected immunologically in strains from a variety of world-wide locations. This additional protein has also been definitively identified both immunologically and as a PBP in methicillin-resistant strains of S. epidermidis (MRSE). The assay described is rapid, specific and sensitive and has been used to detect PBP 2' in S. haemolyticus but not in beta-lactam resistant Streptococci.
O'Hara
D M
DM
Department of Biochemistry, University of Cambridge, U.K.
Harrington
C R
CR
Reynolds
P E
PE
D000217:Acyltransferases / Q000276:immunology*; D001426:Bacterial Proteins; D002352:Carrier Proteins; D006602:Hexosyltransferases / Q000276:immunology*; D015151:Immunoblotting; D008712:Methicillin / Q000494:pharmacology; D009097:Multienzyme Complexes / Q000276:immunology*; D002267:Muramoylpentapeptide Carboxypeptidase; D010403:Penicillin Resistance; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases / Q000276:immunology*; D013211:Staphylococcus aureus / Q000032:analysis / Q000187:drug effects*; D013212:Staphylococcus epidermidis / Q000032:analysis / Q000187:drug effects*
D001426:Bacterial Proteins; D002352:Carrier Proteins; D009097:Multienzyme Complexes; D046915:Penicillin-Binding Proteins; D000217:Acyltransferases; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase; D008712:Methicillin