title: Expression of enzymatically active enkephalinase (neutral endopeptidase) in mammalian cells.
abstract: A cDNA encoding the rat enkephalinase protein (neutral endopeptidase; EC 3.4.24.11) has been constructed from overlapping lambda gt10 cDNA clones. This cDNA was inserted into an expression plasmid containing the cytomegalovirus enhancer and promoter. When transfected with this plasmid, Cos 7 cells transiently expressed the enkephalinase protein in a membrane-bound state. Recombinant enkephalinase recovered in solubilized extracts from transfected Cos 7 cells was enzymatically active and displayed properties similar to those of the native enzyme with respect to sensitivity to classical enkephalinase inhibitors.
authors.affiliation: Department of Cell Genetics, Genentech, Inc., South San-Francisco, California 94080.
authors.forename: C M
authors.initials: CM
authors.lastname: Gorman
authors.forename: D
authors.initials: D
authors.lastname: Gies
authors.forename: P R
authors.initials: PR
authors.lastname: Schofield
authors.forename: H
authors.initials: H
authors.lastname: Kado-Fong
authors.forename: B
authors.initials: B
authors.lastname: Malfroy
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004791:Enzyme Inhibitors; D005786:Gene Expression Regulation; D015260:Neprilysin / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics*; D010957:Plasmids; D051381:Rats; D011994:Recombinant Proteins / Q000235:genetics
chemical_list: D004791:Enzyme Inhibitors; D011994:Recombinant Proteins; D015260:Neprilysin