title: Solid-phase synthesis of oligoribonucleotides using 9-fluorenylmethoxycarbonyl (Fmoc) for 5'-hydroxyl protection.
abstract: Efficient solid-phase synthesis of a series of oligoribonucleotides of up to 20 residues is described that utilises the 9-fluorenylmethoxycarbonyl group (Fmoc) for 5'-protection and 4-methoxytetrahydropyran-4-yl (Mthp) for 2'-protection of ribonucleotide monomers and a phosphoramidite coupling procedure. The Fmoc group is removed after each coupling step by treatment with 0.1M DBU in acetonitrile. Oligoribonucleotides are isolated in 2'-protected form in good yield and shown to be readily and efficiently deprotected by mild acidic treatment.
authors.affiliation: Medical Research Council, Laboratory of Molecular Biology, Cambridge, UK.
authors.forename: C
authors.initials: C
authors.lastname: Lehmann
authors.forename: Y Z
authors.initials: YZ
authors.lastname: Xu
authors.forename: C
authors.initials: C
authors.lastname: Christodoulou
authors.forename: Z K
authors.initials: ZK
authors.lastname: Tan
authors.forename: M J
authors.initials: MJ
authors.lastname: Gait
mesh_terms: D001483:Base Sequence; D003432:Cross-Linking Reagents; D005449:Fluorenes; D008969:Molecular Sequence Data; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D010755:Organophosphates; D009943:Organophosphorus Compounds / Q000138:chemical synthesis; D012263:Ribonucleosides / Q000138:chemical synthesis; D013387:Succinic Anhydrides
chemical_list: D003432:Cross-Linking Reagents; D005449:Fluorenes; D009843:Oligoribonucleotides; D010755:Organophosphates; D009943:Organophosphorus Compounds; D012263:Ribonucleosides; D013387:Succinic Anhydrides