Definition of subchromosomal intervals around the myotonic dystrophy gene region at 19q.
The localization to 19q of the gene causing myotonic dystrophy (DM) has been defined more precisely by refinement of the physical location of several linked markers. A somatic cell hybrid mapping panel from cells with t(1;19), t(12;19), and t(X;19) translocation products was constructed to define five different intervals across 19q. In addition, we have derived a series of cell hybrids by irradiation of a der(19)-only hybrid to further subdivide the cen-q13.1 region. Using an array of 36 cloned genes, anonymous DNAs, and enzyme markers, we have tested the location of the panel breakpoints and refined the regional assignment of several of these markers. All markers tightly linked to DM are localized mainly within 19q13.2, thus suggesting that the DM gene is also close to this region.
Schonk
D
D
Department of Human Genetics, University of Nijmegen, Radboud Hospital, The Netherlands.
Coerwinkel-Driessen
M
M
van Dalen
I
I
Oerlemans
F
F
Smeets
B
B
Schepens
J
J
Hulsebos
T
T
Cockburn
D
D
Boyd
Y
Y
Davis
M
M
D000818:Animals; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19 / Q000648:ultrastructure*; D006224:Cricetinae; D015342:DNA Probes; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D009223:Myotonic Dystrophy / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D015342:DNA Probes; D005819:Genetic Markers