Proliferative response of peripheral blood mononuclear cells to autologous and allogeneic muscle in patients with polymyositis/dermatomyositis.
We examined the proliferative responses of peripheral blood mononuclear cells (PBMC) to autologous and homologous muscle homogenates in 21 patients with early, active, untreated polymyositis/dermatomyositis (PM/DM), 8 patients with chronic PM/DM, 10 patients with myopathies other than PM/DM, 7 patients with connective tissue diseases without myositis, and 12 healthy individuals. PBMC from patients with PM/DM and from control subjects were incubated with various dilutions of autologous and homologous muscle homogenates. PBMC from patients with active PM/DM underwent significant proliferation on exposure to both the autologous muscle and the homologous muscle homogenates. In contrast, PBMC from patients with chronic PM/DM, other myopathies, connective tissue diseases without myositis, and from healthy individuals did not respond to either autologous or homologous muscle. Our findings demonstrate that the PBMC of patients with PM/DM are sensitized to muscle.
Kalovidouris
A E
AE
Department of Medicine, Veterans Administration Medical Center, Indianapolis, IN 46202.
Pourmand
R
R
Passo
M H
MH
Plotkin
Z
Z
D000293:Adolescent; D000328:Adult; D000368:Aged; D001706:Biopsy; D002455:Cell Division; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D003882:Dermatomyositis / Q000473:pathology*; D005260:Female; D006801:Humans; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology*; D009132:Muscles / Q000473:pathology*; D009220:Myositis / Q000473:pathology*