Cytogenetic and molecular studies in primary myelofibrosis.
Cytogenetic and molecular data of three patients affected by primary myelofibrosis with myeloid metaplasia (PMMM) evolving to blastic crisis are reported. The cytogenetic findings were uncommon. The first patient (female) showed an idic(X)(q13) as the sole alteration in chronic phase, with an additional r(7) in 67% of the cells of the blast crisis; the other two patients showed, in blast crisis, a partial trisomy of the long arm of chromosome 1, without translocation, as a unique structural abnormality. These findings confirm the presence of nonrandom, although nonspecific, alterations in PMMM that, in our cases, seem to be related to the multistep progression of the neoplastic process. Molecular investigations have been applied to study the genomic organization and the level of expression of genes such as bcr and calcyclin and c-fms protooncogene possibly involved in the molecular mechanisms underlying cell proliferation in hematopoietic cells. The data obtained are discussed with respect to the myeloproliferative disorder.
Emilia
G
G
Second Medical Clinic, University of Modena, Italy.
Temperani
P
P
Ferrari
S
S
Zucchini
P
P
Tagliafico
E
E
Selleri
L
L
Torelli
G
G
Artusi
T
T
Torelli
U
U
D000368:Aged; D002135:Calcium-Binding Proteins / Q000235:genetics; D018797:Cell Cycle Proteins; D002869:Chromosome Aberrations; D005260:Female; D015321:Gene Rearrangement; D006801:Humans; D007621:Karyotyping; D008297:Male; D008875:Middle Aged; D055728:Primary Myelofibrosis / Q000235:genetics*; D011519:Proto-Oncogenes; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins
D002135:Calcium-Binding Proteins; D018797:Cell Cycle Proteins; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins; C055351:S100A6 protein, human