[The effects and properties of sodium nucleinate as a pyrogen working standard. 6. Pyrogen inactivation by incubation with blood serum or blood plasma].
The 24-h storage of horse serum at 4 degrees C to which bacterial lipopolysaccharide (P) or sodium nucleinate (NN) was added, did not change the pyrogenic effect of these mixtures. Even after 2-h incubation at 37 degrees C changes could not be clearly detected. 24-h incubation at 37 degrees C leads to a clear, but only in case of NN, statistically significant reduction of pyrogenic effect. 24-h incubation of human heparin plasma at 37 degrees C, to which P or NN was added, in both cases resulted in statistically significant reductions of pyrogenic effect. Thus, when incubated with horse serum or human heparin plasma, the pyrogenic effect of NN is reduced in principle as well as that of P treated in the same way.
Dressel
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Institut für Arzneimittelwesen der DDR, Berlin.
D000818:Animals; D001831:Body Temperature / Q000187:drug effects; D004926:Escherichia coli / Q000032:analysis; D006736:Horses; D006801:Humans; D009696:Nucleic Acids / Q000097:blood / Q000494:pharmacology*; D011135:Polysaccharides, Bacterial / Q000097:blood; D011749:Pyrogens / Q000097:blood; D011817:Rabbits; D012015:Reference Standards
D009696:Nucleic Acids; D011135:Polysaccharides, Bacterial; D011749:Pyrogens; C013683:sodium nucleinate
Uber Wirkungen und Eigenschaften des als Pyrogen-Arbeitsstandard verwendeten Natriumnucleinats. 6. Mitteilung: Pyrogen-Inaktivierung durch Inkubation mit Blutserum bzw. Blutplasma.