Avidin-biotin enhanced reverse passive hemagglutination method for detection of HIV antigens.
A new assay for HIV antigens, the avidin-biotin enhanced reverse passive hemagglutination (AB RPHA) test is described which is suitable for detection of infection in cell culture experiments. This assay is simple to perform and economical, and has sensitivity equal to or greater than that of commercial ELISA assays. The coated red cells used for this assay may be stored in the frozen or lyophilized state.
Xu
J Y
JY
Lindsley F. Kimball Research Institute, New York Blood Center, NY 10021.
Prince
A M
AM
D001360:Avidin; D001710:Biotin; D003365:Costs and Cost Analysis; D004797:Enzyme-Linked Immunosorbent Assay; D015488:HIV Antigens / Q000032:analysis*; D006386:Hemagglutination Tests / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011237:Predictive Value of Tests
D015488:HIV Antigens; D001360:Avidin; D001710:Biotin