Does the lack of regression-associated mRNA expression render a rat ventral prostate epithelial cell line androgen independent?
A series of rapidly dividing epithelial (RDE) cell lines have been isolated from primary cultures of rat ventral prostate (RVP) epithelial cells. Unlike androgen-dependent secretory epithelial cells, the RDE cells in culture do not express the androgen-dependent secretory proteins, nor do they express the androgen-repressed cell death sequences (TRPM-2) found in the epithelial cells during prostatic regression. Screening of a cDNA clone library established from RDE cell mRNA has yielded a number of RDE cell-specific sequences. One of these, RDE-.25 is a 250-base mRNA. The sequence of RDE-.25 shows considerable homology with the rat growth hormone gene and two murine oncogene sequences. We believe that the absence of androgen-repressed cell death sequence expression confers androgen independence for survival and growth, while the expression of RDE-.25 may represent an autocrine growth stimulus which greatly increases the rate of cell division in these cells.
Montpetit
M L
ML
Department of Biochemistry, University of Ottawa, Ontario, Canada.
Tenniswood
M P
MP
D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004247:DNA / Q000032:analysis; D004847:Epithelial Cells; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D011467:Prostate / Q000166:cytology / Q000187:drug effects*; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats
D000728:Androgens; D012333:RNA, Messenger; D004247:DNA