title: Relative cerebral ischemia in SHR due to hypotensive hemorrhage: cerebral function, blood flow and extracellular levels of lactate and purine catabolites.
abstract: Cerebral blood flow (CBF, by laser Doppler flowmetry) and extracellular cortical concentrations (by microdialysis) of adenosine, inosine, xanthine, hypoxanthine, and lactate were measured together with somatosensory evoked potentials (SEP) in chloralose-anaesthetized spontaneously hypertensive rats (SHR) during relative cerebral ischemia induced by hypotensive hemorrhage. Reduction of mean arterial blood pressure (MABP) to 40-50 mm Hg, which decreased SEP to about 50% of prebleeding control level, decreased CBF only to about 75% of control due to cerebrovascular "autoregulation." A secondary, marked rise in cerebrovascular resistance (CVR) occurred after about 15 min in parallel with a striking increase in heart rate (after initial bradycardia). This late rise in heart rate is probably elicited by relative ischemia in medullary centers. The increase in CVR might indicate increased sympathetic nerve activity to the circle of Willis and large cerebral arteries. Cortical lactate increased initially but started to decline after about 30 min, and after 2 h it was not significantly higher than control. Cortical adenosine, inosine, hypoxanthine, and xanthine increased slowly and were significantly elevated after 50 min of hemorrhage. After 80 min, adenosine and inosine had returned to initial levels, while hypoxanthine and xanthine were further elevated. Despite the apparent partial recovery of metabolic disturbances during late hemorrhage, and with a blood flow maintained at 75% of resting control, SEP did not improve. It is suggested that the depression of SEP is not primarily caused by circulatory-metabolic derangements, but instead by activation of specific inhibitory systems.
authors.affiliation: Department of Physiology, University of Göteborg, Sweden.
authors.forename: J O
authors.initials: JO
authors.lastname: Skarphedinsson
authors.forename: M
authors.initials: M
authors.lastname: Sandberg
authors.forename: H
authors.initials: H
authors.lastname: Hagberg
authors.forename: S
authors.initials: S
authors.lastname: Carlsson
authors.forename: P
authors.initials: P
authors.lastname: Thorén
mesh_terms: D000241:Adenosine / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000503:physiopathology*; D002545:Brain Ischemia / Q000209:etiology / Q000503:physiopathology*; D002543:Cerebral Hemorrhage / Q000150:complications*; D002560:Cerebrovascular Circulation; D005110:Extracellular Space / Q000378:metabolism; D006973:Hypertension / Q000150:complications*; D007022:Hypotension / Q000150:complications*; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000378:metabolism; D007288:Inosine / Q000378:metabolism; D007700:Kinetics; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008297:Male; D051381:Rats; D011918:Rats, Inbred SHR; D019820:Xanthine; D014970:Xanthines / Q000378:metabolism
chemical_list: D007042:Hypoxanthines; D007773:Lactates; D014970:Xanthines; D019820:Xanthine; D019271:Hypoxanthine; D019344:Lactic Acid; D007288:Inosine; D000241:Adenosine