Size distribution and general structural features of N-linked oligosaccharides from the methylotrophic yeast, Pichia pastoris.
The secreted glycoproteins of Pichia pastoris contain more than 35% of their N-linked oligosaccharides as structures smaller than Man14GlcNAc2 (Man = mannose; GlcNAc = N-acetylglucosamine). On heterologous invertase produced in P. pastoris, approximately 85% of the oligosaccharides are in the size range Man8-14GlcNAc2. The structures appear to contain alpha-linked mannose. In addition, one-third of the structures contain net negative charge and can be radio-labelled in vivo with 32P. The largest oligosaccharides isolated from P. pastoris are significantly shorter than the hypermannosylated structures typical of S. cerevisiae, indicating that the factors which influence the processing of N-linked oligosaccharides in P. pastoris are different from those which influence processing in S. cerevisiae. The smaller N-linked oligosaccharides synthesized by P. pastoris resemble high-mannose oligosaccharides synthesized by animal cells, and this finding increases the utility of P. pastoris as a host for the production of heterologous glycoproteins.
Grinna
L S
LS
Salk Institute Biotechnology/Industrial Associates, Inc., La Jolla, California 92037.
Tschopp
J F
JF
D002458:Cell Fractionation; D002850:Chromatography, Gel; D006023:Glycoproteins / Q000032:analysis; D006026:Glycoside Hydrolases / Q000096:biosynthesis / Q000378:metabolism; D006031:Glycosylation; D009844:Oligosaccharides / Q000032:analysis*; D010843:Pichia / Q000032:analysis* / Q000201:enzymology / Q000378:metabolism; D004718:Saccharomycetales / Q000032:analysis*; D043324:beta-Fructofuranosidase
D006023:Glycoproteins; D009844:Oligosaccharides; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase