Improved TLC systems for rapid resolution of phenyl thiohydantoin amino acids.
Two new solvent systems, n-hexane + propionic acid (26:5, v/v) and chloroform + acetone (29:3, v/v), for the rapid resolution and identification of an 18-component mixture of phenylthiohydantoin amino acids are reported. Using these systems certain difficult combinations of phenylthiohydantoin amino acids are resolved. Two more solvent systems, viz chloroform + acetic acid (27:3, v/v) and chloroform + methanol (30:4, v/v), are developed to resolve phenylthiodantoin derivatives of aspartic and glutamic acids.
Bhushan
R
R
Department of Chemistry, University of Roorkee, India.
Mahesh
V K
VK
Mallikharjun
P V
PV
D000596:Amino Acids / Q000032:analysis*; D002855:Chromatography, Thin Layer; D006827:Hydantoins / Q000032:analysis*; D007202:Indicators and Reagents; D010669:Phenylthiohydantoin / Q000031:analogs & derivatives / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D000596:Amino Acids; D006827:Hydantoins; D007202:Indicators and Reagents; D010669:Phenylthiohydantoin