Aleuria aurantia agglutinin. A new isolation procedure and further study of its specificity towards various glycopeptides and oligosaccharides.
A new procedure for isolating a L-fucose-specific lectin from the mushroom Aleuria aurantia is described. The fine specificity of the purified lectin was determined by inhibition of agglutination of human red blood cells by various glycopeptides and oligosaccharides, and by studying the affinity of the immobilized lectin towards glycopeptides and oligosaccharides. Results of inhibition of hemagglutination showed that the lectin presents the highest affinity towards alpha-(1----6)-linked L-fucosyl groups. Immobilized Aleuria aurantia agglutinin interacts strongly with all N-glycosylpeptides or related glycans possessing an alpha-L-fucopyranosyl group linked to O-6 of the 2-acetamido-2-deoxy-beta-D-glucopyranosyl residue involved in the glycosylamine linkage. In addition, presence of alpha-(1----3)-linked L-fucosyl groups greatly enhances the affinity of the lectin for the alpha-(1----6)-L-fucosylated glycans. The immobilized Aleuria lectin is a powerful tool for the resolution of the microheterogeneity of L-fucosylated glycopeptides and glycans of the N-acetyl-lactosamine type.
Debray
H
H
Laboratoire de Chimie Biologique de l'Université des Sciences et Techniques de Lille Flandres-Artois, Laboratoire Pilote du Ministère de l'Education Nationale et Unité Associée au CNRS No. 217, Villeneuve d'Ascq, France.
Montreuil
J
J
D000373:Agglutinins / Q000302:isolation & purification; D001216:Asparagine; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D005643:Fucose; D006020:Glycopeptides; D006386:Hemagglutination Tests; D006801:Humans; D008970:Molecular Weight; D009844:Oligosaccharides; D014168:Transferrin; D005644:alpha-L-Fucosidase / Q000378:metabolism
D000373:Agglutinins; D006020:Glycopeptides; D009844:Oligosaccharides; D014168:Transferrin; D005643:Fucose; D001216:Asparagine; D005644:alpha-L-Fucosidase