title: Presence of tumour necrosis factor or a related factor in human basophil/mast cells.
abstract: The observation that mast cell products and cachectin/tumour necrosis factor (TNF) mediate similar responses suggested an investigation of cultured human basophil/mast cells for production of TNF. Using in situ hybridization and the avidin biotin-complex (ABC) immunoperoxidase method, we have demonstrated the presence of TNF mRNA in the cytoplasm and TNF protein in the granules of individual human basophil/mast cells. The production of TNF by these cells could explain many of their reported functions.
authors.affiliation: Department of Developmental Hematopoiesis, Memorial Sloan-Kettering Cancer Center, New York, New York.
authors.forename: M
authors.initials: M
authors.lastname: Steffen
authors.forename: M
authors.initials: M
authors.lastname: Abboud
authors.forename: G K
authors.initials: GK
authors.lastname: Potter
authors.forename: Y P
authors.initials: YP
authors.lastname: Yung
authors.forename: M A
authors.initials: MA
authors.lastname: Moore
mesh_terms: D001491:Basophils / Q000378:metabolism*; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism; D006801:Humans; D008407:Mast Cells / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D014409:Tumor Necrosis Factor-alpha