Detection of single-copy genes by nonisotopic in situ hybridization on human chromosomes.
A technique of in situ hybridization on metaphase chromosomes with biotinylated DNA probes is described. This technique was used to localize unique DNA sequences on chromosomes and allowed a localization of two probes 1.8 and 1.3 kb long. The hybridization signal appears like two, twin, spots on the two sister chromatids, allowing a clear distinction from the background. Moreover a chromosomal localization is possible by counting a relatively small number of mitoses compared with the technique using 3H-labeled DNA probes.
Cherif
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Unité INSERM U.301, Hôpital Scint-Louis, Paris, France.
Bernard
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O
Berger
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R
D001710:Biotin; D002877:Chromosomes, Human / Q000648:ultrastructure*; D004247:DNA / Q000235:genetics*; D015342:DNA Probes; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization
D015342:DNA Probes; D001710:Biotin; D004247:DNA