Vanadium(IV)-stimulated hydrolysis of 2,3-diphosphoglycerate.
Vanadium(IV) stimulates the hydrolysis of 2,3-diphosphoglycerate at 23 degrees C. The pH optimum is 5.0. Reactions were analyzed by enzymatic and phosphate release assays. The products of 2,3-diphosphoglycerate hydrolysis are inorganic phosphate and 3-phosphoglycerate. The reaction is inhibited by high concentrations of 2,3-diphosphoglycerate and an equation has been formulated that describes the kinetic constants for this reaction at pH 7. The possible relevance of the reaction to the therapeutic lowering by vanadium(IV) of red cell 2,3-diphosphoglycerate in sickle-cell disease is discussed.
Stankiewicz
P J
PJ
Department of Chemistry, Simon Fraser University, British Columbia, Canada.
D019794:2,3-Diphosphoglycerate; D003545:Cysteine / Q000494:pharmacology; D004163:Diphosphoglyceric Acids / Q000032:analysis*; D006019:Glycolysis; D006861:Hydrogen Peroxide / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D014639:Vanadium / Q000494:pharmacology*
D004163:Diphosphoglyceric Acids; D014639:Vanadium; D019794:2,3-Diphosphoglycerate; D006861:Hydrogen Peroxide; D003545:Cysteine