title: Interaction of E1040 with cephalosporinase from Citrobacter freundii GN7391.
abstract: The interactions of E1040 with cephalosporinase from Citrobacter freundii, including affinity and hydrolysis, were studied in comparison with those of cefotaxime and ceftazidime. E1040 showed a higher stability at low drug concentrations and a much lower affinity for the enzyme than did cefotaxime or ceftazidime. These enzymological properties explain the high activity of E1040 against cephalosporinase-producing C. freundii.
authors.affiliation: Episome Institute, Gunma, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Inoue
authors.forename: S
authors.initials: S
authors.lastname: Mitsuhashi
mesh_terms: D002439:Cefotaxime / Q000494:pharmacology; D002442:Ceftazidime / Q000494:pharmacology; D002510:Cephalosporinase / Q000378:metabolism*; D002511:Cephalosporins / Q000378:metabolism*; D002954:Citrobacter / Q000201:enzymology*; D007700:Kinetics; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D002511:Cephalosporins; C056494:cefclidin; D002442:Ceftazidime; D002510:Cephalosporinase; D001618:beta-Lactamases; D002439:Cefotaxime