Binding of bullous pemphigoid and pemphigus vulgaris sera to SCaBER cell line in an ELISA.
Specific antibodies present in the sera of patients with bullous pemphigoid or pemphigus vulgaris were detected in an enzyme-linked immunosorbent assay (ELISA) employing a squamous carcinoma cell line, SCaBER, as substrate. Bullous pemphigoid sera bound preferentially to permeabilized cells, suggesting that the antigens are largely intracellular. The assay may prove to be a useful addition to current methods of detecting circulating antibodies in these patients.
Cook
A L
AL
Dermatology Research Unit E5, Addenbrooke's Hospital, Cambridge, U.K.
Mallett
R B
RB
Hanahoe
T H
TH
Pye
R J
RJ
D000937:Antigen-Antibody Reactions; D001323:Autoantibodies / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000276:immunology*; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D010391:Pemphigoid, Bullous / Q000097:blood / Q000276:immunology*; D010392:Pemphigus / Q000097:blood / Q000276:immunology*; D012872:Skin Diseases, Vesiculobullous / Q000276:immunology*; D001749:Urinary Bladder Neoplasms / Q000276:immunology
D001323:Autoantibodies