title: Changes in ovine conceptus and endometrial function following asynchronous embryo transfer or administration of progesterone.
abstract: The secretory protein profile from conceptuses collected from naturally mated ewes on Days 10, 12, 14, and 16 was characterized by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and fluorography. The presence of the anti-luteolysin ovine trophoblast protein-1 (oTP-1) in culture medium from Day 10 conceptuses was confirmed by fluorography, Western blotting, and radioimmunoassay (RIA). On each of the days studied, oTP-1 was the dominant secretory protein, and was secreted in increasing quantities as pregnancy progressed. In a second experiment, Day 6 embryos were transferred to either Day 6 (SR) or Day 4 (AR) recipients. Three mated ewes (P) received daily injections of 50 mg progesterone on Days 4-9. Controls consisted of 2 groups of pregnant ewes (D8 and D10). Conceptuses and ipsilateral endometrium were collected 4 days following transfer of conceptuses to SR and AR ewes, on Day 10 in P and D10 ewes, and on Day 8 in D8 ewes. Conceptus volume was estimated upon recovery from the uterus. Tissues were cultured with 35S-methionine, and the medium was analyzed for total and trichloroacetic acid-precipitable radiolabeled proteins. Levels of specific endometrial secretory proteins were determined after protein separation by 2D-PAGE and estimation of the radioactivity associated with discrete radiolabeled proteins on fluorographs. The concentration of oTP-1 in conceptus culture medium was estimated by RIA. Thirty endometrial proteins were investigated. All 30 proteins were present in endometrial cultures from SR, AR, D10, and P ewes, but 13 proteins were absent from D8 ewes. Levels of three proteins were higher in AR compared to D8 (p less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Animal Science Department, University of Florida, Gainesville 32611.
authors.forename: C J
authors.initials: CJ
authors.lastname: Ashworth
authors.forename: F W
authors.initials: FW
authors.lastname: Bazer
mesh_terms: D000818:Animals; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000502:physiology; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology*; D005260:Female; D005865:Gestational Age; D007370:Interferon Type I; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000032:analysis / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology*; D012756:Sheep
chemical_list: D007370:Interferon Type I; D011257:Pregnancy Proteins; C020947:interferon tau; D011374:Progesterone