title: The exogastrula-inducing peptides in embryos of the sea urchin, Anthocidaris crassispina--isolation and determination of the primary structure.
abstract: Four exogastrula-inducing peptides, A, B, C, and D have been isolated from the homogenates of embryos of the sea urchin, Anthocidaris crassispina, with successive chromatographic fractionations. The complete amino acid sequences of the peptides A and D were determined by analysis of the peptides generated by their digestion with lysyl endopeptidase. They were composed of 52 and 53 amino acid residues, and their molecular weights were calculated to be 5754 and 5737, respectively. The sequences of peptides A and D were DSVYQCNRDTNSCDGFGKCEKSTFGRTTGQYICNCDDGYRNNAYGGCSPRTE, and DTVARCERDTKNCDGHGTCQLSTFGRRTGQYICFCDAGYRKPNSYGGCSPSSA, respectively. The biological significance of the exogastrula-inducing peptides was discussed.
authors.affiliation: Department of Regulation Biology, Faculty of Science, Saitama University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Suyemitsu
authors.forename: T
authors.initials: T
authors.lastname: Asami-Yoshizumi
authors.forename: S
authors.initials: S
authors.lastname: Noguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Tonegawa
authors.forename: K
authors.initials: K
authors.lastname: Ishihara
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005775:Gastrula / Q000187:drug effects; D007447:Invertebrate Hormones / Q000032:analysis / Q000302:isolation & purification*; D008297:Male; D008666:Metalloendopeptidases; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010455:Peptides / Q000032:analysis / Q000302:isolation & purification; D012617:Sea Urchins / Q000196:embryology*
chemical_list: D007447:Invertebrate Hormones; D010455:Peptides; C061708:exogastrula-inducing peptide A, Anthocidaris crassispina; C061709:exogastrula-inducing peptide D, Anthocidaris crassispina; D008666:Metalloendopeptidases