Expression of c-myc protooncogene in primary cultures of human corneal stromal cells.
We have determined steady-state levels of c-myc mRNA in quiescent and serum-stimulated human corneal stromal cells. Steady-state levels of c-myc mRNA increased 6-fold following 2 hours of serum stimulation over levels observed at quiescence. A parallel increase in the rate of c-myc gene transcription was observed in serum-stimulated cells as compared to quiescent cells, indicating that the abundance of c-myc transcripts in corneal stromal cells during the transition from quiescence to proliferation is regulated mainly at the transcriptional level. These findings indicated that the expression of c-myc gene in human corneal stromal cells is regulated in a cell growth dependent manner in response to serum induction.
Rao
G N
GN
Department of Ophthalmology, Emory University School of Medicine, Atlanta, GA 30322.
Broeckx
J L
JL
Church
R L
RL
D003315:Cornea / Q000378:metabolism*; D003319:Corneal Stroma / Q000187:drug effects / Q000378:metabolism*; D003513:Cycloheximide / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D015342:DNA Probes; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D006801:Humans; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000302:isolation & purification; D014158:Transcription, Genetic
D015342:DNA Probes; D012333:RNA, Messenger; D004247:DNA; D003513:Cycloheximide