Lipid metabolism of rat adipocytes in culture.
Isolated differentiated rat adipocytes embedded in a collagen matrix were maintained in culture for 0, 4 and 14 days. Assessment of viability by either anabolic glucose metabolism or catabolic lipolytic metabolism can be misleading; both types of metabolism should be measured to establish the status of cells. Cells maintained morphological integrity even when not viable as judged by anabolic glucose metabolism.
Hu
C Y
CY
Department of Animal Sciences, University of Illinois, Urbana 61801.
Novakofski
J E
JE
Mersmann
H J
HJ
D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D003094:Collagen; D005227:Fatty Acids / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism; D050356:Lipid Metabolism; D008055:Lipids / Q000096:biosynthesis; D008066:Lipolysis; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D005227:Fatty Acids; D008055:Lipids; D003094:Collagen; D005947:Glucose; D005990:Glycerol