Effect of cisplatin on con A agglutinability of different lymphoid cells of Swiss albino rats.
Thymocytes, splenocytes, and lymph node cells showed a differential degree of agglutination which con A, which was the lowest for thymocytes and the highest for lymph node cells. Cisplatin treatment of the cells showed a gradual increase and then decrease in the degree of agglutination of the cells which varied with cisplatin concentration and time of treatment. The cells treated with 20 micrograms/ml of cisplatin for 30 min showed the maximum increase in the degree of con A agglutination. Incubation of these cisplatin-treated cells with D-xylose, D-glucose, or sialic acid before con A agglutination showed a decrease in the cell agglutinability which was the lowest for sialic acid-incubated cells. It is suggested that the removal of cell surface carbohydrate moieties after cisplatin treatment may play a role in the changes in con A agglutination of these cells.
Prasad
S B
SB
Department of Zoology, North Eastern Hill University, Shillong, India.
D000818:Animals; D002449:Cell Aggregation / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology*; D003208:Concanavalin A / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology; D066298:In Vitro Techniques; D008198:Lymph Nodes / Q000276:immunology; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects; D019158:N-Acetylneuraminic Acid; D051381:Rats; D012794:Sialic Acids / Q000494:pharmacology; D013154:Spleen / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology; D014994:Xylose / Q000494:pharmacology
D012794:Sialic Acids; D003208:Concanavalin A; D014994:Xylose; D019158:N-Acetylneuraminic Acid; D005947:Glucose; D002945:Cisplatin