Regional localization and molecular characterization of a DNA sequence on the long arm of chromosome 22.
A human genomic DNA fragment, p22hom13 (D22S16), was isolated from a chromosome 22-specific library. After elimination of repetitive sequences, a single copy BamHI-EcoRI fragment was subcloned into pTZ18. By using mouse/human somatic cell hybrids and in situ hybridization, the new DNA probe was mapped to chromosome 22q13-qter. Its application in the analysis of the distal part of chromosome 22 and its diagnostic use in translocations are discussed.
Göttert
E
E
Institut für Humangenetik, Universitätsklinik, Homburg/Saar, Federal Republic of Germany.
Metzdorf
R
R
Färber
U
U
Blin
N
N
D002874:Chromosome Mapping; D002892:Chromosomes, Human, Pair 22; D015342:DNA Probes / Q000302:isolation & purification*; D006801:Humans; D006822:Hybrid Cells; D015151:Immunoblotting; D009693:Nucleic Acid Hybridization; D011995:Recombination, Genetic
D015342:DNA Probes