Message amplification phenotyping (MAPPing): a technique to simultaneously measure multiple mRNAs from small numbers of cells.
A rapid and highly sensitive technique (MAPPing: message amplification phenotyping) has been developed to simultaneously analyze the array of messenger RNAs made by small numbers of cells. The technique incorporates a micro-procedure for isolating RNA, reverse transcription of total cellular RNA to produce cDNA, and enzymatic amplification of cytokine-specific DNA fragments using the polymerase chain reaction. In this study, the technique has been applied to the analysis of cytokines produced by lymphoid cells ranging in number from a single cell to 10(6) cells. The technique should be applicable to virtually any tissue or cell type.
Brenner
C A
CA
Genelabs Inc., Redwood City, CA 94063.
Tam
A W
AW
Nelson
P A
PA
Engleman
E G
EG
Suzuki
N
N
Fry
K E
KE
Larrick
J W
JW
D001483:Base Sequence; D001685:Biological Factors / Q000235:genetics; D001709:Biotechnology; D002460:Cell Line; D016207:Cytokines; D004247:DNA / Q000235:genetics; D005821:Genetic Techniques; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics* / Q000378:metabolism
D001685:Biological Factors; D016207:Cytokines; D012333:RNA, Messenger; D004247:DNA