title: [Study of the composition of cell wall of group A Streptococcus after hydrolysis using muramidase from Streptomyces levoris].
abstract: The aim of the experiment was to study the lysis products of cell walls of group A streptococci resulting from exposure to N-acetylmuramidase. It was shown that for isolating surface proteins free of polysaccharide and peptidoglycan fragments it was necessary to treat the streptococcal cell walls with endo-beta-N-acetylmuramidase for no more than 30 minutes. Prolonged hydrolysis with muramidase led to the presence of polysaccharide and the peptidoglycan fragments in the protein fractions, intracellular wall proteins covalently bound to the peptidoglycan fragments and polysaccharide being also released.
authors.forename: Z F
authors.initials: ZF
authors.lastname: Shmakova
authors.forename: E P
authors.initials: EP
authors.lastname: Save'lev
authors.forename: V D
authors.initials: VD
authors.lastname: Kuznetsov
authors.forename: L O
authors.initials: LO
authors.lastname: Dynga
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis / Q000378:metabolism*; D002473:Cell Wall / Q000032:analysis / Q000378:metabolism; D003470:Culture Media; D006026:Glycoside Hydrolases / Q000494:pharmacology*; D006868:Hydrolysis; D066298:In Vitro Techniques; D009113:Muramidase / Q000494:pharmacology*; D010457:Peptidoglycan / Q000032:analysis / Q000378:metabolism*; D013297:Streptococcus pyogenes / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D013302:Streptomyces / Q000201:enzymology*; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D010457:Peptidoglycan; D006026:Glycoside Hydrolases; C026255:endo-N-acetylmuramidase; D009113:Muramidase
vernacular_title: Izuchenie sostava kletochnoĭ stenki streptokokka gruppy A pri posledovate'lnom gidrolize muramidazoĭ Streptomyces levoris.