Activation of murine peritoneal macrophages by saikosaponin a, saikosaponin d and saikogenin d.
Macrophage activation by saikosaponins and saikogenins was investigated and compared with that by other saponins and macrophage stimulants. Saikosaponins a and d induced a marked cell accumulation in the peritoneal cavity when administered intraperitoneally. Among saikosaponins and saikogenins tested, saikosaponin d significantly activated peritoneal macrophages in terms of enhancement of phagocytic activity, increased level of cellular lysosomal enzyme (acid phosphatase), induction of cytostatic activity and expression of Ia antigen on the cell surface. The activities of saikosaponin d were much stronger than those of typical saponins ginsenoside Rg1 and glycyrrhizin and almost comparable with or somewhat weaker than those of lipopolysaccharide, a streptococcal preparation OK-432 and formalin-killed Propionibacterium acnes, indicating that saikosaponin d is a potent macrophage activator.
Kumazawa
Y
Y
School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.
Takimoto
H
H
Nishimura
C
C
Kawakita
T
T
Nomoto
K
K
D000818:Animals; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D007274:Injections, Intraperitoneal; D008247:Lysosomes / Q000201:enzymology; D008262:Macrophage Activation / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D015394:Molecular Structure; D009828:Oleanolic Acid / Q000031:analogs & derivatives*; D012502:Sapogenins / Q000494:pharmacology*; D012503:Saponins
D000949:Histocompatibility Antigens Class II; D012502:Sapogenins; D012503:Saponins; D009828:Oleanolic Acid; C025759:saikosaponin D