Correlation of exposure time, concentration and incorporation of IdUrd in V-79 cells with radiation response.
These experiments were designed to find the minimum concentration at which incorporation of and sensitization by IdUrd (Iododeoxyuridine) would occur and the effect of concentrations from .1 to 100 microM for exposures of 8 to 96 hr in cultured V-79 cells exposed to 137Cs gamma rays at 2 Gy per minute. At 0.1 microM thymidine replacement averaged 1% and the SER ranged from 1.1 to 1.28, significant at the 95% level. The maximum thymidine replacement was 49% after 48 hr exposure to 30 microM yielding an SER of 2.7. SER generally peaked after 72 hr of exposure. This cell line has an 8 hr cycle time in our hands and thus optimal sensitization would occur after 9 cell cycles. These ranges need testing in human cells in culture and in Phase I clinical trials.
Phillips
T L
TL
Laboratory of Radiobiology, UCSF 94143.
Bodell
W J
WJ
Uhl
V
V
Ross
G Y
GY
Rasmussen
J
J
Mitchell
J B
JB
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D006224:Cricetinae; D007065:Idoxuridine / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D011836:Radiation Tolerance; D011838:Radiation-Sensitizing Agents / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D013997:Time Factors
D011838:Radiation-Sensitizing Agents; D007065:Idoxuridine