Non-radioactive chemical sequencing of biotin labelled DNA.
Methods for the nonradioactive chemical sequencing of DNA are described. A biotin marker molecule, attached chemically to an oligonucleotide primer or enzymatically in an endfilling reaction of restriction enzyme sites, is stable during the base-specific chemical modification and strand scission reactions. Following fragment separation by direct blotting electrophoresis, the membrane bound sequence pattern can be visualized by a streptavidin-bridged enzymatic color reaction. The biotin labeling is also applicable for DNA sequencing by random degradation of phosphothioates, thus showing to be a universal label for nonradioactive DNA sequencing.
Richterich
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Fakultät für Biologie, Universität Konstanz, FRG.
D001426:Bacterial Proteins; D001483:Base Sequence; D001710:Biotin; D055598:Chemical Phenomena; D002621:Chemistry; D003124:Colorimetry; D004247:DNA; D004586:Electrophoresis; D004852:Epoxy Compounds; D063086:Organothiophosphates; D020005:Propanols; D019809:Streptavidin
D001426:Bacterial Proteins; D004852:Epoxy Compounds; D063086:Organothiophosphates; D020005:Propanols; D001710:Biotin; D004247:DNA; D019809:Streptavidin; C004312:glycidol