Analysis of the individual allergens of Russian thistle pollen by an enzyme-linked immunoblotting technique.
Russian thistle pollen extract was analyzed by immunoblots of isoelectric focused and SDS-PAGE gels. Twenty distinct protein bands were recognized by human IgE- and IgG-specific antibodies in the immunoblot from the SDS-PAGE gel. Molecular weights of these allergens ranged from 12.2 kD to 85 kD. Seventeen bands were detected on isoelectric focusing immunoblots with pI from 3.95 to 7.70. Allergic subjects had differing individual patterns of protein band recognition. Immunoblot techniques provide detailed evaluations of the response of allergy subjects to components of crude natural allergens.
Gomez
J
J
University of Texas, El Paso.
Mansfield
L E
LE
Frederick
R W
RW
Rael
E D
ED
D000485:Allergens / Q000032:analysis* / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D007525:Isoelectric Focusing; D008970:Molecular Weight; D011058:Pollen; D012882:Skin Tests
D000485:Allergens