title: DNA probes bind non-specifically to eosinophils during in situ hybridization: carbol chromotrope blocks binding to eosinophils but does not inhibit hybridization to specific nucleotide sequences.
abstract: Eosinophils were found to bind radiolabelled DNA probes non-specifically during in situ hybridization. Pretreatment of cells with carbol chromotrope blocked non-specific binding without interfering with the recognition of specific nucleotide sequences.
authors.affiliation: Division of Immunological Medicine, Clinical Research Centre, Harrow, Middlesex, U.K.
authors.forename: S
authors.initials: S
authors.lastname: Patterson
authors.forename: J
authors.initials: J
authors.lastname: Gross
authors.forename: A D
authors.initials: AD
authors.lastname: Webster
mesh_terms: D015342:DNA Probes; D004279:DNA, Viral / Q000378:metabolism*; D004804:Eosinophils / Q000378:metabolism*; D006801:Humans; D007202:Indicators and Reagents; D009693:Nucleic Acid Hybridization
chemical_list: D015342:DNA Probes; D004279:DNA, Viral; D007202:Indicators and Reagents