Halobacterium sp. GRB: a species to work with!?
The properties of the halobacterial isolate Halobacterium sp. GRB are discussed, especially in relation to its use as a laboratory strain. Experimental results on this species are described, including the isolation of point mutants in the bacterioopsin gene leading to single amino acid replacements in bacteriorhodopsin, the application of a selection procedure for the isolation of different types of mutants, the genetic stability of Halobacterium sp. GRB and the possibility of isolating a set of isogenic mutants, the conditions for transformation experiments with this species, and specific features of Halobacterium sp. GRB, such as halocin production and the absence of a restriction system, as well as DNA adenosine methylation.
Soppa
J
J
Max-Planck-Institut für Biochemie, Martinsried, Federal Republic of Germany.
Oesterhelt
D
D
D001436:Bacteriorhodopsins / Q000235:genetics; D001973:Bromodeoxyuridine; D005798:Genes, Bacterial; D006217:Halobacterium / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D013045:Species Specificity; D014170:Transformation, Genetic
D001436:Bacteriorhodopsins; C017827:bacterio-opsin; D001973:Bromodeoxyuridine