Long term bone marrow culture in metastatic neuroblastoma.
We have developed a long term marrow culture assay for the study of advanced bone marrow metastatic neuroblastoma. In this in vitro system the hemopoietic growth (GM-CFU) is not affected by the presence of tumor cells. The neuroblastoma cells grow and differentiate partially when in contact with the stromal layer, arresting the culture. We present culture and histological data suggesting that the solid tumors interact specifically with the stromal layer of marrow origin.
Thierry
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D
D.P.S., S.H.R., I.P.S.N., C.E.A., B.P.6, Fontenay aux Roses, France.
Validire
P
P
Hardy
M
M
Magdelenat
H
H
Zucker
J M
JM
D001853:Bone Marrow / Q000201:enzymology / Q000473:pathology*; D002450:Cell Communication; D002478:Cells, Cultured; D002675:Child, Preschool; D003114:Colony-Forming Units Assay; D006801:Humans; D008854:Microscopy, Electron; D009447:Neuroblastoma / Q000473:pathology* / Q000556:secondary; D010751:Phosphopyruvate Hydratase / Q000032:analysis; D013997:Time Factors
D010751:Phosphopyruvate Hydratase