title: Clindamycin and tetracycline as immunomodulating agents: an in vivo study.
abstract: The present study was undertaken to determine the effects of clindamycin and tetracycline, both intravenously administered, on antibody response to thymus-dependent antigen (PC-KLH) in BALB/c mice. The immunological parameters evaluated were: DPFC/spleen (direct plaque forming-cells), antibody secretion median rate (PC50), heterogeneity index (Hi), number of total splenic lymphocytes and cellular viability. The results showed that clindamycin (i.v.) increased the humoral response; 28 mg/kg was the dose that showed the greatest enhancement (+73%). The PC50 was not affected by clindamycin but Hi decreased at 28 mg/kg and increased at 2.8 mg/kg doses, although neither result was statistically significant. When tetracycline was given i.v., a slight decrease in the anti-PC DPFC number was observed. Although the PC50 was greater at 10 mg/kg (p less than 0.05), Hi was smaller at the 1 mg/kg dose (p less than 0.05).
authors.affiliation: Department of Microbiology, Faculty of Medicine, Complutense University, Madrid, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Corrales
authors.forename: A
authors.initials: A
authors.lastname: Suarez
authors.forename: A
authors.initials: A
authors.lastname: Lima
authors.forename: S
authors.initials: S
authors.lastname: Ballestero
authors.forename: M L
authors.initials: ML
authors.lastname: Gómez-Lus
authors.forename: J
authors.initials: J
authors.lastname: Prieto
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D002981:Clindamycin / Q000494:pharmacology*; D005260:Female; D006433:Hemocyanins / Q000276:immunology; D006462:Hemolytic Plaque Technique; D007166:Immunosuppressive Agents; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010713:Phosphatidylcholines / Q000494:pharmacology; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D013752:Tetracycline / Q000494:pharmacology*
chemical_list: D000276:Adjuvants, Immunologic; D007166:Immunosuppressive Agents; D010713:Phosphatidylcholines; D002981:Clindamycin; D006433:Hemocyanins; D013752:Tetracycline