title: Crystallization of a synthetic analog of calcium-binding site III of rabbit skeletal troponin C.
abstract: Single crystals of the calcium-binding peptide AcA98STnC (90-123)amide, which is a synthetic fragment analog of rabbit skeletal troponin C calcium-binding site III, have been grown in the tetragonal crystal system. Crystals were developed to a maximum size of 0.60 x 0.60 x 0.75-mm by the technique of washing and reseeding. The space group is I41 with a = 40.21(1) A, c = 34.07(1) A; there is 1 polypeptide fragment/crystallographic asymmetric unit, and the solvent content is estimated to be 29%. The crystals diffract to at least 1.8-A d spacings and are stable in the x-ray beam for 1 week.
authors.affiliation: Department of Biochemistry, University of Saskatchewan, Saskatoon, Canada.
authors.forename: L T
authors.initials: LT
authors.lastname: Delbaere
authors.forename: M
authors.initials: M
authors.lastname: Vandonselaar
authors.forename: R E
authors.initials: RE
authors.lastname: Reid
mesh_terms: D000818:Animals; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism*; D003460:Crystallization; D009132:Muscles / Q000378:metabolism*; D010455:Peptides / Q000302:isolation & purification / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D011817:Rabbits; D014336:Troponin / Q000378:metabolism*; D019209:Troponin C; D014961:X-Ray Diffraction
chemical_list: D010455:Peptides; D014336:Troponin; D019209:Troponin C; D002118:Calcium