title: Isolation of phenol-degrading Bacillus stearothermophilus and partial characterization of the phenol hydroxylase.
abstract: Bacillus stearothermophilus BR219, isolated from river sediment, degraded phenol at levels to 15 mM at a rate of 0.85 mumol/h (4 x 10(6) cells). The solubilized phenol hydroxylase was NADH dependent, exhibited a 55 degrees C temperature optimum for activity, and was not inhibited by 0.5 mM phenol.
authors.affiliation: Department of Microbiology and Public Health, Michigan State University, East Lansing 48824-1101.
authors.forename: G
authors.initials: G
authors.lastname: Gurujeyalakshmi
authors.forename: P
authors.initials: P
authors.lastname: Oriel
mesh_terms: D001411:Geobacillus stearothermophilus / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification*; D007700:Kinetics; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D010636:Phenols / Q000378:metabolism; D012988:Soil Microbiology
chemical_list: D010636:Phenols; D006899:Mixed Function Oxygenases; C022336:phenol 2-monooxygenase