Fatal infection of silvered leaf monkeys with a virus-like infectious agent (VLIA) derived from a patient with AIDS.
Four silvered leaf monkeys, inoculated with a virus-like infectious agent (VLIA) derived from transformed NIH/3T3 cells (sb51) transfected with Kaposi's sarcoma DNA of an AIDS patient, showed wasting syndromes and died in 7-9 months. Two monkeys had a transient lymphadenopathy in earlier stages. Two moribund animals showed lymphopenia. Although 3 of the VLIA inoculated monkeys had persistent low grade fever early in the infection, the animals became afebrile in the later stages. One VLIA inoculated animal had a prominent antibody response, which occurred 7 months after VLIA inoculation. The other 3 monkeys had a transient or poor antibody response in the later stages. These 3 animals revealed periodic VLIA antigenemia during the course of the experiment. A control monkey was killed 8 months after the last VLIA inoculated monkey succumbed and showed neither an antibody response nor evidence of antigenemia. VLIA-specific DNA could be directly detected in necropsy tissues of all 4 monkeys inoculated with VLIA using the polymerase chain reaction method. VLIA infection was identified in all 4 spleens, 2 of 4 livers, 1 of 2 kidneys, and all 3 brains tested from these 4 animals, but not in the tissues from the control monkey. The necropsy examination of the 4 VLIA inoculated animals revealed no opportunistic infections, acute inflammatory lesions, malignancy or cause of death other than VLIA infection. We believe that the VLIA caused a fatal systemic infection in these monkeys.
Lo
S C
SC
Department of Infectious and Parasitic Diseases Pathology, Armed Forces Institute of Pathology, Washington, DC.
Wang
R Y
RY
Newton
P B
PB
Yang
N Y
NY
Sonoda
M A
MA
Shih
J W
JW
D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology*; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002520:Cercopithecidae; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005784:Gene Amplification; D006801:Humans; D007958:Leukocyte Count; D008099:Liver / Q000648:ultrastructure; D008297:Male; D014162:Transfection; D014777:Virus Diseases / Q000097:blood / Q000382:microbiology / Q000401:mortality*; D014780:Viruses / Q000032:analysis / Q000235:genetics / Q000648:ultrastructure
D000956:Antigens, Viral; D004279:DNA, Viral