The effects of dose of elemental mercury and first-pass circulation time on exhalation and organ distribution of inorganic mercury in rats.
The lung plays a major role in the removal of dissolved elemental mercury (Hg0) from the bloodstream. During the first passage through the lung after an intravenous dose of Hg0 dissolved in aqueous buffer, from 10 to 17% was exhaled depending on the dose (0.11 or 1.1 micrograms Hg/rat) and the injection site (jugular versus tail vein). Furthermore, evidence is presented that subsequent exhalation over the next 50 s, before the rats were killed and the mercury determined in the lung at that time, was largely Hg0-extracted during the first pass. The total mercury extracted during the 60 s period was in the range of 40-49% of the dose. The oxidation of Hg0 to Hg2+ in red cells is important in limiting the availability of Hg0 to certain tissues. Thus, after a short residence time in blood (0.6 s after jugular vein injection), 12.9-17% is exhaled in the first pass as compared to 10.4-12.2% with a longer residence time (1.8 s after tail vein injection). Furthermore, there was a general tendency, even at 60 s after dosing, for certain tissues - lung, brain, and heart - to have higher values after dosing from the jugular vein. It was estimated that the half-time for oxidation was 3.3 s. Our results confirm previous observations that the form of inorganic mercury greatly influences the short-term deposition in certain tissues. Thus as compared to Hg2+, administration of Hg0 increases lung levels 5-10-fold; brain, 4-fold; and heart, 3-fold. Blood levels are lower after Hg0, particularly after the higher dose. Such findings are consistent with a model wherein Hg0 is in part oxidized by red blood cells, the remainder rapidly diffusing in tissues where it is also oxidized to Hg2+.
Magos
L
L
MRC Toxicology Unit, MRC Laboratories, Carshalton U.K.
Clarkson
T W
TW
Hudson
A R
AR
D000818:Animals; D001944:Breath Tests; D002412:Cations; D004305:Dose-Response Relationship, Drug; D007275:Injections, Intravenous; D007601:Jugular Veins; D008168:Lung / Q000032:analysis / Q000502:physiology*; D008297:Male; D008628:Mercury / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D010084:Oxidation-Reduction; D011652:Pulmonary Circulation; D051381:Rats; D011919:Rats, Inbred Strains; D013623:Tail / Q000098:blood supply; D013997:Time Factors; D014018:Tissue Distribution; D014692:Ventilation-Perfusion Ratio
D002412:Cations; D008628:Mercury