Effect of sodium butyrate, a differentiating agent, on cell surface glycoconjugates of a human pancreatic cell line.
The effects of sodium butyrate, a differentiating agent, on growth properties and glycoconjugates of a human pancreatic carcinoma cell line (CAPAN-1) were studied. Butyrate caused marked changes of in vitro growth properties including prolongation of doubling time and greatly reduced colony forming efficiency in soft agar. Cell surface labeling revealed significant alterations in proteins and glycoproteins after treatment with sodium butyrate (appearance of glycoproteins of molecular weight 250,000, 220,000, and 70,000; reduction of glycoproteins of 164,000, 148,000, 110,000, and 66,800 sizes; increases in proteins/glycoproteins of 85,000 and 78,000). Metabolic labeling of the cells with [3H]fucose or [3H]galactose revealed that sodium butyrate treatment caused a marked reduction in the fucose-containing neutral glycolipids with six or more carbohydrate side chains and an increase in [3H]galactose-labeled neutral glycolipids, particularly GL-3a, GL-4a, and GL-5a. There was marked reduction in the labeling of a ganglioside with mobility similar to that of GM4 and of sulfogalactosylceramide. An increase in a ganglioside with mobility above GM1 was also caused by butyrate. These data indicate that sodium butyrate may be useful in the identification of differentiation or malignancy-associated glycoconjugate markers of human pancreatic cells.
Bloom
E J
EJ
Gastrointestinal Research Laboratory, Veterans Administration Medical Center, San Francisco, CA 94121.
Siddiqui
B
B
Hicks
J W
JW
Kim
Y S
YS
D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002462:Cell Membrane / Q000032:analysis / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D006001:Glycoconjugates / Q000378:metabolism*; D006017:Glycolipids / Q000378:metabolism; D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism; D008563:Membrane Lipids / Q000378:metabolism; D010179:Pancreas / Q000166:cytology*; D010190:Pancreatic Neoplasms / Q000473:pathology
D002087:Butyrates; D006001:Glycoconjugates; D006017:Glycolipids; D008562:Membrane Glycoproteins; D008563:Membrane Lipids; D020148:Butyric Acid