title: Identification and developmental expression of a novel embryonic myosin heavy-chain gene in chicken.
abstract: The developmental expression of an embryonic chicken myosin heavy-chain (MHC) gene homologous to the genomic clone pCM4.1 was examined by S1 analysis. Transcripts homologous to pCM4.1 are first detected at day 12 in ovo, and are maximally expressed between days 15-17 in ovo. No pCM4.1 transcripts are detected at earlier stages of embryogenesis or at high levels in posthatch stages. This unique pattern of expression has led to the proposal that pCM4.1 represents a previously uncharacterized MHC gene, which is confined in its expression to late embryogenesis. Genomic hybridization data, in addition to a comparison between the DNA and amino acid sequences of pCM4.1 and other characterized chicken MHC 3' end clones, provide further evidence for this proposal. We also present observations made during the sequence analysis of pCM4.1 that may be relevant to our understanding of the 3'-end processing of homologous primary transcripts, and of the mechanism controlling developmental MHC isoform transitions.
authors.affiliation: Department of Molecular and Cell Biology, University of Connecticut, Storrs 06268.
authors.forename: A A
authors.initials: AA
authors.lastname: Lagrutta
authors.forename: J G
authors.initials: JG
authors.lastname: McCarthy
authors.forename: C A
authors.initials: CA
authors.lastname: Scherczinger
authors.forename: S M
authors.initials: SM
authors.lastname: Heywood
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002642:Chick Embryo; D004247:DNA / Q000302:isolation & purification*; D015342:DNA Probes; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D009218:Myosins / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D015342:DNA Probes; D004247:DNA; D009218:Myosins