Angiotensin II receptors in the gonads.
The presence of components of the renin-angiotensin system in ovaries and testes suggests that angiotensin II (AII) is involved in gonadal function, and thus we sought to characterize receptors for AII in rat and primate gonads. In the testes, autoradiographic studies showed receptors in the interstitium in all species. In rat interstitial cells fractionated by Percoll gradient, AII receptors coincided with hCG receptors indicating that AII receptors are located on the Leydig cells. In Leydig cells and membranes from rat and rhesus monkey prepuberal testes, AII receptors were specific for AII analogues and of high affinity (Kd=nM). During development, AII receptor content in rat testes decreases with age parallel to a fall in the ratio of interstitial to tubular tissue. In the ovary, the distribution of AII receptors was dependent on the stage of development, being high in the germinal epithelium and stromal tissue between five and 15 days, and becoming localized in secondary follicles in 20-and 40-day-old rats. No binding was found in primordial or primary follicles. In rhesus monkey ovary, AII receptors were higher in stromal tissue and lower in granulosa and luteal cells of the follicles. Characterization of the binding in rat and monkey ovarian membranes showed a single class of sites with a Kd in the nmol/L range and specificity similar to that of the adrenal glomerulosa and testicular AII receptors. Receptors for AII were also present in membrane fractions from PMSG/hCG primed rat ovaries. Infusion of AII (25 ng/min) or captopril (1.4 micrograms/min) during the PMSG/hCG induction period had no effect on ovarian weight or AII receptor concentration in the ovaries.(ABSTRACT TRUNCATED AT 250 WORDS)
Aguilera
G
G
Reproduction Research Branch, National Institute of Child Health and Human Development, Bethesda, Maryland 20892.
Millan
M A
MA
Harwood
J P
JP
D000804:Angiotensin II / Q000378:metabolism*; D000818:Animals; D001345:Autoradiography; D002428:Cebus; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D005260:Female; D006064:Gonadotropins, Equine / Q000494:pharmacology; D006066:Gonads / Q000378:metabolism / Q000648:ultrastructure*; D006801:Humans; D007457:Iodine Radioisotopes; D008253:Macaca mulatta; D008297:Male; D010053:Ovary / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D011945:Receptors, Angiotensin / Q000032:analysis* / Q000378:metabolism; D013737:Testis / Q000378:metabolism / Q000648:ultrastructure
D006063:Chorionic Gonadotropin; D006064:Gonadotropins, Equine; D007457:Iodine Radioisotopes; D011945:Receptors, Angiotensin; D000804:Angiotensin II