Interaction between phage G13 and its oligosaccharide receptor studied by equilibrium dialysis.
The reversible binding of phage G13, a phi X174-like single-strand DNA phage, to a 3H-labelled nonasaccharide from the lipopolysaccharide of its natural host Escherichia coli C was studied with equilibrium dialysis. The binding constant (Ka) was determined to 1.3 x 10(7) M-1 in Scatchard and Lineweaver-Burk plots. Approximately one saccharide bound per G13 phage particle which suggests that only one of the 12 spikes in each G13 virion was engaged in the phage/receptor saccharide interaction. Equilibrium dialysis inhibition experiments with saccharides from lipopolysaccharides of an isogenic series of Salmonella typhimurium mutants showed that hepta- and pentasaccharides from two G13-sensitive bacteria, i.e., with efficiencies of plating of 0.1-1.0 compared to E. coli C, were efficient inhibitors with Ka-values greater than or equal to 1.2 x 10(7) M-1. The octa- and hexasaccharides from two G13 resistant strains, with efficiency of plating less than or equal to x 10(-4), were either greater than 1000-fold or greater than 15-fold less efficient as inhibitors with Ka-values less than or equal to 8.8 x 10(5) M-1. The results show that phage G13 binds in a specific and reversible way to penta-, hepta-, and nonasaccharides from G13 sensitive bacteria with the specificity residing in the hexose and heptose region of the core lipopolysaccharide.
Bruse
G W
GW
National Bacteriological Laboratory, Department of Bacteriology, Stockholm, Sweden.
Wollin
R
R
Lindberg
A A
AA
D055598:Chemical Phenomena; D002621:Chemistry; D003090:Coliphages / Q000378:metabolism*; D003956:Dialysis; D004926:Escherichia coli / Q000378:metabolism*; D008024:Ligands; D009844:Oligosaccharides / Q000378:metabolism*; D011991:Receptors, Virus / Q000378:metabolism*; D012486:Salmonella typhimurium / Q000378:metabolism*
D008024:Ligands; D009844:Oligosaccharides; D011991:Receptors, Virus