Multifrequency phase fluorescence study of hapten-antibody complexation.
The rotational dynamics of a phenytoin fluoroimmunoassay system is investigated by using multifrequency phase and modulation fluorescence spectroscopy. Results indicated that the fluorescein label on this relatively small hapten undergoes significant (greater than 45%) local motion even when bound to the phenytoin antibody. We attribute this local motion to unhindered rotation of the fluorescein moiety around the bond attaching it to phenytoin. This local motion affects the sensitivity of a steady-state polarization immunoassay dramatically.
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D000906:Antibodies; D005453:Fluorescence; D006241:Haptens; D008433:Mathematics
D000906:Antibodies; D006241:Haptens