title: Appearance of lectin-binding sites during vascularization of the primordium of the central nervous system in 10 to 12-day-old mouse embryos.
abstract: In the present study lectin-binding sites were investigated for the lectins Ricinus communis agglutinin (RCA I), wheat germ agglutinin (WGA), soya bean agglutinin (SBA), concanavalin A (Con A), Lotus tetragonolobus-(LTA) and Limulus polyphemus agglutinin (LPA) during the initial stages of vasculogenesis of the CNS-anlage in 10 to 12-day-old NMRI mouse embryos. Specific binding sites for the lectins RCA I (sugar specificity: beta-D-galactose, N-acetylgalactosamine), WGA (sugar specificity: N-acetylglucosamine, sialic acid), and SBA (sugar specificity: N-acetylgalactosamine, beta-D-galactose) were detected in the newly formed capillaries within the neuroepithelial cell layer. In contrast, binding sites for Con A, LTA and LPA could not be observed at the start of the vascularization of the CNS-anlage. From these results, the conclusion can be drawn that glycoconjugates containing D-galactose, N-acetylgalactosamine and N-acetyl-glucosamine moieties are involved in the early vasculogenesis of the embryonic CNS-anlage of the mouse.
authors.affiliation: Abt. Histologie, Georg-August-Universität Göttingen, Federal Republic of Germany.
authors.forename: H B
authors.initials: HB
authors.lastname: Welim
authors.forename: M
authors.initials: M
authors.lastname: Thies
authors.forename: R
authors.initials: R
authors.lastname: Herken
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D002490:Central Nervous System / Q000098:blood supply / Q000196:embryology* / Q000378:metabolism; D005314:Embryonic and Fetal Development; D037102:Lectins / Q000378:metabolism*; D051379:Mice
chemical_list: D037102:Lectins