Effect of 8-anilino-1-naphthalene sulfonic acid (ANS) on the interaction between thyroid hormone and anti-thyroid hormone antibodies.
Effect of 8-anilino-1-naphthalene sulfonic acid (ANS) on the interaction between thyroid hormone and anti-thyroid hormone antibodies was examined. Addition of 5.1 x 10(6) or 6.3 x 10(6) molar excess of ANS, respectively, enhanced the binding of 125I-T3 or 125I-T4 to their respective autoantibodies. However, further increase in ANS concentration resulted in a decrease in binding. These results suggest that the optimum concentration of ANS for the detection of anti-thyroid hormone antibodies is 5 approximately 6 x 10(6) molar excess of ANS to 125I-T3 or 125I-T4. On the other hand, addition of 10(4) molar excess of ANS concentration decreased the binding of 125I-insulin to anti-insulin antibodies. It was therefore suggested that the effect of ANS could be a nonspecific one and likely due to its negative charge.
Matsuda
M
M
Third Department of Internal Medicine, Gifu University School of Medicine, Japan.
Sakata
S
S
Komaki
T
T
Nakamura
S
S
Kojima
N
N
Takuno
H
H
Miura
K
K
D000817:Anilino Naphthalenesulfonates / Q000494:pharmacology*; D000906:Antibodies / Q000276:immunology*; D000937:Antigen-Antibody Reactions / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006111:Graves Disease / Q000276:immunology; D006801:Humans; D007328:Insulin / Q000276:immunology; D013974:Thyroxine / Q000276:immunology* / Q000378:metabolism; D014284:Triiodothyronine / Q000276:immunology* / Q000378:metabolism
D000817:Anilino Naphthalenesulfonates; D000906:Antibodies; D007328:Insulin; D014284:Triiodothyronine; C035763:5,5'-bis(8-(phenylamino)-1-naphthalenesulfonate); D013974:Thyroxine