Influence of sample concentration and adsorption time on the yield of biomolecule ions in plasma desorption mass spectrometry.
The yield of intact ions formed in 252Cf plasma desorption mass spectrometry has been investigated by analyzing melittin and bovine trypsin at different concentrations on a nitrocellulose surface. The yield of trypsin ions is shown to vary with the protein concentration and adsorption time. The singly charged ion of trypsin is observed when concentrated solution (10 microM to 1 mM) of bovine trypsin are applied for sufficient time.
Craig
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AG
Bennich
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H
D000327:Adsorption; D000818:Animals; D002417:Cattle; D013058:Mass Spectrometry / Q000379:methods*; D014357:Trypsin / Q000032:analysis
D014357:Trypsin