title: Primary structure of rat liver elongation factor 2 deduced from the cDNA sequence.
abstract: A number of cDNA clones in summary encoding 700 amino acid residues from the N-end of rat liver elongation factor 2 (EF-2) and including 49 nucleotides of the 5'-untranslated mRNA region have been obtained. EF-2 cDNA clones were isolated from gradually constructed small (1000-5000 clones) specific cDNA libraries using the primer extension method for synthesis of the first cDNA chain. The complete primary structure of cDNA and protein EF-2 from rat liver was derived taking into account the primary structure of the 3'-terminal region EF-2 cDNA previously reported [(1986) Proc. Natl. Acad. Sci. USA 83, 4978-4982]. Comparison of this cDNA with hamster cDNA has shown that (i) the base sequences had a 89.7% homology while that of the 5'-untranslated region was 73%; (ii) there are two amino acid replacement in rat liver EF-2 as compared with hamster EF-2.
authors.affiliation: Institute of Protein Research, Academy of Sciences of the USSR, Moscow Region.
authors.forename: A V
authors.initials: AV
authors.lastname: Oleinikov
authors.forename: G G
authors.initials: GG
authors.lastname: Jokhadze
authors.lastname: Alakhov YuB
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D006224:Cricetinae; D004247:DNA / Q000302:isolation & purification*; D008099:Liver / Q000378:metabolism*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000302:isolation & purification*; D051381:Rats
chemical_list: D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors; D012333:RNA, Messenger; D004247:DNA