Reciprocal regulation of tachykinin- and vasoactive intestinal peptide-gene expression in rat sensory neurones following cut and crush injury.
The relative abundance of preprotachykinin- (PPT), actin- and vasoactive intestinal peptide- (VIP) mRNA's was measured in L5 dorsal root ganglia of rats after resecting or crushing the sciatic nerve. PPT-mRNA levels fell to 40% of control values 3, 6 and 9 days following nerve resection. Crushing produced a lesser fall at 3 and 6 days with a partial recovery at 9 days. Following resection actin-mRNA levels transiently rose to twice control values and had returned to normal by day 9. VIP-mRNA was not detectable in control ganglia but increasing amounts of VIP-mRNA were present 3, 6 and 9 days after nerve injury. The results are discussed in terms of the control mechanisms operating.
Nielsch
U
U
Department of Pharmacology, Medical School, University of Bristol, U.K.
Keen
P
P
D000199:Actins / Q000378:metabolism; D000818:Animals; D005727:Ganglia, Spinal / Q000378:metabolism* / Q000502:physiology; D005786:Gene Expression Regulation; D008297:Male; D009409:Nerve Crush; D009416:Nerve Regeneration; D009475:Neurons, Afferent / Q000378:metabolism* / Q000502:physiology; D011498:Protein Precursors / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D015320:Tachykinins / Q000378:metabolism*; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*
D000199:Actins; D011498:Protein Precursors; D012333:RNA, Messenger; D015320:Tachykinins; C044206:preprotachykinin; D014660:Vasoactive Intestinal Peptide