A simple method for detection of viral satellite RNAs in small plant tissue samples.
A procedure is described that allows extraction and can estimate the total amount of single-stranded and double-stranded viral satellite RNAs and viral RNA present in a minimal amount of infected plant tissue, and is capable of distinguishing different cucumber mosaic viral and nepoviral satellites by polyacrylamide gel electrophoresis under semi-denaturing or fully-denaturing conditions.
White
J L
JL
Dept. of Botany, University of Maryland, College Park.
Kaper
J M
JM
D015152:Blotting, Northern; D004591:Electrophoresis, Polyacrylamide Gel; D007887:Fabaceae; D009029:Mosaic Viruses / Q000235:genetics*; D010944:Plants / Q000382:microbiology*; D010946:Plants, Medicinal; D010947:Plants, Toxic; D012330:RNA, Double-Stranded / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis*; D012526:Satellite Viruses / Q000235:genetics*; D014026:Nicotiana; D014675:Vegetables
D012330:RNA, Double-Stranded; D012367:RNA, Viral