Comparison of dot enzyme-linked immunosorbent assay (Dot-ELISA), passive haemagglutination test (PHT) and thin layer immunoassay (TIA) in the diagnosis of natural or experimental Fasciola hepatica infections in sheep.
The dot enzyme-linked immunosorbent assay (Dot-ELISA) was compared with the passive haemagglutination test (PHT) and thin layer immunoassay (TIA) for the detection of antibodies against Fasciola hepatica in naturally and experimentally infected sheep. The infected animals gave titres from 1:25,000 to 1:204,000, while control animals gave titres of from 1:100 to 1:800. The titres of the infected sheep obtained by Dot-ELISA were 1000-2000 times higher than the ones obtained using TIA or PHT. Due to its high sensitivity, this technique could be very useful for the diagnosis of ovine fascioliasis.
Arriaga de Morilla
C
C
Department of Immunology, Instituto Nacional de Investigaciones Forestales y Agropecuarias, México, D.F.
Paniagua
R
R
Ruíz-Navarrete
A
A
Bautista
C R
CR
Morilla
A
A
D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005210:Fasciola hepatica / Q000276:immunology*; D005211:Fascioliasis / Q000175:diagnosis / Q000662:veterinary*; D006386:Hemagglutination Tests; D007118:Immunoassay; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis*
D000909:Antibodies, Helminth