[Quantitative immunoenzyme determination of lactoferrin in the serum and plasma].
A procedure for quantitative estimation of lactoferrin is developed using the "sandwich" type of immunoenzymatic assay. Preparation of lactoferrin was purified from women colostrum by means of ion exchange chromatography; the sorbent (a ligand-containing complex of lactoferrin) was synthesized; the antibody conjugate against lactoferrin and peroxidase from horseradish was prepared; optimal conditions of the immunoenzymatic assay were selected. Concentration of lactoferrin in donor blood serum or plasma constituted 0.5-3.3 mg/ml; distinct alterations of the lactoferrin content were not found in blood of patients maintained on artificial circulation or in the blood used for transfusion.
Merkulov
A G
AG
Shevchenko
O P
OP
D006801:Humans; D007124:Immunoenzyme Techniques; D007781:Lactoferrin / Q000097:blood*; D007782:Lactoglobulins / Q000097:blood*
D007782:Lactoglobulins; D007781:Lactoferrin
Kolichestvennoe immunofermentnoe opredelenie laktoferrina v syvorotke i plazme krovi.