The role of macrophages in the immunoadjuvant action of liposomes: effects of elimination of splenic macrophages on the immune response against intravenously injected liposome-associated albumin antigen.
The primary antibody response to intravenously administered and liposome-associated human serum albumin (HSA) was studied in mice under conditions where no response could be detected against the non-liposome-associated form of the antigen. The positive response against the antigen, entrapped in and/or exposed on the surfaces of liposomes, thus resulted from the adjuvant action of the liposomes. In mice intravenously injected with dichloromethylene diphosphonate (C12MDP) also entrapped in liposomes, all red pulp macrophages, marginal metallophilic macrophages and marginal zone macrophages had disappeared from the spleen 2 days after administration. Twenty-two days after such a treatment red pulp macrophages and marginal metallophilic macrophages had reappeared, but marginal zone macrophages were still absent. In mice injected with liposome-associated HSA at 2 days after treatment with the C12MDP liposomes, anti-HSA responses were severely depressed, but administration of the liposome-associated antigen 22 days after C12MDP liposomes elicited a normal response. These results point to a role of splenic macrophages in the processing of liposome-associated antigens, but marginal zone macrophages, which are located close to the open ends of the white pulp capillaries and thus are the first macrophages to meet the antigens arriving in the marginal zone are not required.
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Department of Histology, Medical Faculty, Free University, Amsterdam, The Netherlands.
Van Rooijen
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D000276:Adjuvants, Immunologic; D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology; D007275:Injections, Intravenous; D008081:Liposomes / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D012709:Serum Albumin / Q000276:immunology*; D013154:Spleen / Q000276:immunology
D000276:Adjuvants, Immunologic; D000941:Antigens; D008081:Liposomes; D012709:Serum Albumin