A 31P NMR study of the GI tract: effect of fructose loading and measurement of transverse relaxation times.
The effect of fructose loading on high-energy phosphates in the jejunum, ileum, and large intestine of rats was studied using 31P NMR. Following fructose loading, an increase in the intensity of the PME resonance was observed in the jejunum, indicating an accumulation of fructose-1-phosphate. There were no significant changes in ATP or Pi. This demonstrates that the activity of fructokinase in the jejunum can be monitored by 31P NMR. Fructose loading had no detectable effect on metabolite levels in the ileum and large intestine. Resolution of intestinal spectra was poor due to unusually large linewidths and the presence of broad underlying signals. To study the mechanism of line broadening, the T2's of the phosphorus resonances were measured using a solenoidal coil. The T2's of the ATP, Pi, PME, and PCr resonances were much longer than the T2's, suggesting that the linewidths of these resonances are primarily due to susceptibility gradients and/or compartmentation of metabolites. Other signals, particularly in the PDE region, were homogeneously broadened and had very short T2's. Spin echoes obtained with evolution times of 1 to 4 ms suppressed these broad components, with little loss of intensity in the inhomogeneously broadened resonances; as a result, resolution was improved.
Karczmar
G S
GS
Magnetic Resonance Unit, Veterans Administration Medical Center, San Francisco.
Tavares
N J
NJ
Weiner
M W
MW
D000818:Animals; D005632:Fructose / Q000008:administration & dosage / Q000378:metabolism*; D005636:Fructosephosphates / Q000378:metabolism; D007082:Ileum / Q000378:metabolism*; D007420:Intestine, Large / Q000378:metabolism*; D007583:Jejunum / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D010758:Phosphorus; D051381:Rats; D011919:Rats, Inbred Strains
D005636:Fructosephosphates; D010758:Phosphorus; D005632:Fructose