The mRNA encoding elongation factor 1-alpha (EF-1 alpha) is a major transcript at the midblastula transition in Xenopus.
A Xenopus laevis gastrula cDNA library has been screened in order to identify sequences that are expressed early in development. We find that the mRNA encoding translation elongation factor 1-alpha (EF-1 alpha) is synthesized in very large amounts in the early embryo. Transcription of EF-1 alpha mRNA commences at the midblastula transition (MBT), and new EF-1 alpha protein is synthesized very soon after this, as determined by the association of EF-1 alpha mRNA with polysomes. The nucleotide sequence of a full-length EF-1 alpha cDNA clone and the deduced amino acid sequence of Xenopus EF-1 alpha protein are presented.
Krieg
P A
PA
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.
Varnum
S M
SM
Wormington
W M
WM
Melton
D A
DA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001755:Blastocyst / Q000378:metabolism*; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000235:genetics*; D011132:Polyribosomes / Q000378:metabolism; D015347:RNA Probes; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D014982:Xenopus laevis / Q000196:embryology
D015342:DNA Probes; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors; D015347:RNA Probes; D012333:RNA, Messenger; D004247:DNA