Guinea-pig tracheal epithelium and endothelin.
Removal of the epithelium increased the responsiveness of isolated guinea-pig trachea to the contractile effects of endothelin. This phenomenon was observed in the presence of indomethacin (5 microM), captopril (10 microM), bacitracin (20 micrograms/ml) or leupeptin (50 microM), but was inhibited by phosphoramidon (10 microM). Bacitrain enhanced contraction produced by endothelin. The increased responsiveness of denuded guinea-pig trachea to endothelin may be due to removal of an epithelium-derived phosphoramidon-sensitive peptidase.
Hay
D W
DW
Department of Pharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406-0939.
D000818:Animals; D016232:Endothelins; D004848:Epithelium / Q000502:physiology; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects*; D010455:Peptides / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D011480:Protease Inhibitors / Q000494:pharmacology; D014132:Trachea / Q000187:drug effects / Q000502:physiology
D016232:Endothelins; D010455:Peptides; D011480:Protease Inhibitors