[Monoclonal antibodies Ki-Sp II-13 and VI-2 react with surface membrane of human spermatozoa].
Immunizing Balb-c mice with washed motile human spermatozoa enabled the production of 2 monoclonal spermatozoal antibodies designated Ki-Sp II-13 and Ki-Sp VI-2. Immunohistochemically Ki-Sp VI-2 reacts only with mature spermatozoa. The monoclonal antibody Ki-Sp II-13 recognizes besides spermatozoa also T- and B-lymphocytes. Electronmicroscopically both antibodies react with the surface membrane of spermatozoa in the area of the head, the neck and the proximal part of the tail. Functional tests show a high sperm-agglutinating and sperm-immobilizing activity, using different test systems for Ki-Sp II-13. Both antibodies proved to be IgG. On immunoprecipitation Ki-Sp II-13 was identified as IgG-2a and Ki-Sp VI-2 as IgG1. Immunoprecipitates of SDS-electrophoretically separated and radiolabeled sperm surface antigens revealed a molecular weight of 18 KD for Ki-Sp II-13 and of 24 KD for Ki-Sp VI-2.
Mettler
L
L
Abteilung Frauenheilkunde im Zentrum für Operative Medizin I u. Michaelis-Hebammenschule, Universität zu Kiel, Bundesrepublik Deutschland.
Salmassi
A
A
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000954:Antigens, Surface / Q000276:immunology*; D003429:Cross Reactions; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013094:Spermatozoa / Q000276:immunology* / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface
Monoklonale Antikörper Ki-Sp II-13 und Ki-Sp VI-2 erkennen Oberflächen-Antigene humaner Spermatozoen.